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From: Paul Keltjens :      PAK-at-eo.ine.philips.nl
Date: Mon, 2 Jan 1995 14:23:26 GMT
Subject: Semiconductor application support position at Philips Electron O

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Philips Electron Optics

invites applicators for the position of

Application Support Engineer


an exiting new position created for our major expansion in the micro-electronics
industry.

If you have
- Experience in defect review/yield improvement,
- Background in lithography and CD measurement,
- The ability to communicate, train, and assist users of equipment, and work in
a fledging highly motivated group,
- An enquiry mind with imagination and the drive to see your ideas
implemented,
- PhD in a related area, or equivalent experience,
- Preferably experience with scanning electron microscopy,
- And you would like to have your home base in the Netherlands,

then please send your C.V. without delay to the undersigned.


Renumeration and conditions of employment are attractive for the right person.

Mr. J. Jackman
Philips Electron Optics
Building AAE-1
P.O. Box 218
5600 MD Eindhoven
The Netherlands
Tel. +31 40 766548
Fax. +31 40 766164
E-mail: jjn-at-eo.ine.philips.nl





From: MCMOULDK-at-usthk.ust.hk
Date: 03 Jan 1995 11:54:41 +0800
Subject: SUTW windows for EDX

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Greetings and Happy New Year,

Last year we had a failure of our SUTW (super ultra thin window) on our TEM
EDS detector. This is our second failure of a SUTW, although not on the same
machine (a SEM) and not from the same manufacture. After talking to several
people, it has become apparent that the reliability of the SUTW is not very
good, although manufactures claim otherwise.

I am curious what other users experiences with SUTW windows are. In
particular what are the typical down times when a failure occurs, do you
take any special precautions or modify your operation procedure, would you
put a SUTW (instead of a standard window or turret detector) on an SEM for
instant which is vented frequently using nitrogen?
What was the reason for the failure?



Keith Moulding.

MCPC,
HKUST,
Hong Kong.




From: MR RHM CROSS :      EURC-at-giraffe.ru.ac.za
Date: Tue, 3 Jan 1995 08:18:14 GMT+0200
Subject: safety issue - eyes

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Does anyone have information on eye damage resulting from long-term
use of electron microscopes?

Robin Cross





From: Lehtinen Pirjo :      plehtine-at-butler.cc.tut.fi
Date: Tue, 3 Jan 1995 09:11:10 +0200 (EET)
Subject: STM/AFM

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Does anyone have experience on P4 SPM MDT scanning tunneling microscopes
and atomic force microscopes? These devices are produced by someone at an
institute that used to be a part of Russian Academy of Sciences.


mail me or this listserver if you have ever heard about them, and i'll be
grateful!

plehtine-at-butler.cc.tut.fi






From: Luc Analbers :      L.J.S.Analbers-at-med.ruu.nl
Date: Tue, 03 Jan 1995 08:38:49 +0100
Subject: PBS

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Hello everyone and a happy new year!

Can anyone tell me wich concentration PBS should be used in immune-incubations
for LM and EM?
We got a little bit confused because some books mention to use a 0.1M PBS
solution, and some mention to use 0.01M PBS.
I can imagine that using a ten-fold solution can give problems.
Wich concentration is the best (immuno-fluorescence LM and immuno-EM)?

Please let me know !
Thanks

Luc Analbers

***************************************************************************
* Luc Analbers * E-mail: Analbers-at-med.ruu.nl *
***************************************************************************
* Utrecht University * LLL *
* Medical Faculty * LLL *
* Dept. Medical Physiology & * LLL A *
* Sportsmedicine * LLL AA AA *
* PO-box 80043 * LLL AA AA *
* Zip: 3508 TA * LLLLLAAALLLAAALLL *
* Utrecht The Netherlands * LLLLLAAALLLAAALLLL *
* Tel: 030 - 538911 * AAA AAA *
* Fax: 030 - 539036 * AAA AAA *
***************************************************************************




From: R100186-at-aol.com
Date: Tue, 3 Jan 1995 12:45:56 -0500
Subject: unsubscribe

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Please cancel my subscribtion.
r100186




From: mmace-at-tiac.net (mmace)
Date: Tue, 3 Jan 1995 18:46:33 -0500
Subject: Subscribe

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Message-Id: {m0rPEIw-00010YC-at-pegasus.cc.ucf.edu}

subscribe microscopy





From: Tina Carvalho :      tina-at-ahi.pbrc.Hawaii.Edu
Date: Tue, 3 Jan 1995 17:25:00 -1000 (HST)
Subject: Glut binding to polysaccharides

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Aloha!
What is known about the interaction between glutaraldehyde and
polysaccharides? Does glut bind to polysaccharides in a way that would
cause fluorescence (beyond that which binds to the small amount of
protein that is present in the sample)? From what I understand, it is
known how it binds to protein and that it binds to glycogen, but that
it doesn't fix soluble polysaccharides, is that right? Could anyone help
us out with the chemistry beyond that? Thanks in advance!

And a Happy New Year to you all!

Tina Weatherby Carvalho
Biological EM Facility
University of Hawaii





From: MARTIN SAUNDERS :      ms-at-siva.bris.ac.uk
Date: Wed, 04 Jan 1995 11:01:41 GMT
Subject: Post doc position at Bristol

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Dear all,

I have been asked to post this by the head of my research group (John
Steeds). He would be grateful if you could pass it on to anyone who
might be interested.

Any replies should go direct to him at J.W.Steeds-at-bristol.ac.uk

Happy New Year to you all,

Martin Saunders.

***************************************************************************

POST DOCTORAL RESEARCH POST


DEPARTMENT OF PHYSICS,
UNIVERSITY OF BRISTOL,
UK.


A post doctoral research assistantship is available for research on the
microstructure and processing of unique metastable Ti-Mg alloys produced by
vapour mixing and condensation. The material is made at the Defence
Research Agency (Farnborough, UK) and close collaboration will be required
with research workers at this Research Agency.

In the as-deposited state, the material is a porous, supersaturated solid
solution with a nanoscale microstructure. The research will involve hot
vacuum pressing and thermal treatments to eliminate porosity and obtain an
optimum dispersed phase microstructure.

The hot working will be carried out in the Mechanical Engineering
Department in an Instron servo controlled press equipped with a high vacuum
chamber. The microstructural studies will require the use of high spatial
resolution transmission electron microscopy and associated analytical
techniques in the Physics Department. Some hardness and mechanical testing
will be carried out in collaboration with DRA (Farnborough) to relate the
microstructure to the mechanical properties.

The person to be appointed should be able to provide evidence of proven
ability at relating microstructure, processing and properties in these
novel alloys whilst working in an interdisciplinary team as well as
considerable experience of advanced transmission electron microscopy
techniques.

For more information please contact Professor J.W. Steeds by e-mail at

J.W.Steeds-at-bristol.ac.uk




From: EMLAB-at-opus.mco.edu
Date: Wed, 04 Jan 1995 10:18:39 -0400 (EDT)
Subject: Re: Glut binding to polysaccharides

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Tina,

I do not know about glut/polysaccharides interactions, but glut by itself
does fluroses(sp) at the same (or close to) wavelength as FITC.

Good Luck,

Ed Calomeni




From: Jaime Dant :      jaime-at-borcim.wustl.edu
Date: Wed, 4 Jan 1995 11:25:32 -0600
Subject: Bubbles in formvar coated grids

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Quick question!
I am getting small bubbles in my formvar ciated grids. How do I get rid of
these bubbles? I am not overtly introducing moisture onto my glass slides
am I am using EM Sciences prepared 0.25% formvar from a new bottle!!!

I don't want to make a project out of this, so input is appreciated.

Thank you for your time.

Jaime A. Dant
jaime-at-borcim.wustl.edu

Sorry about the typo, thats formvar coated grids.




From: sfzane-at-unccvm.uncc.edu (Sandra F. Zane)
Date: Wed, 4 Jan 1995 13:46:08 -0500
Subject: Bubbles in Formvar

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Hello Jaime and all,
In the way of a suggestion, you might try a new source for your formvar
solution. I had the same problem after about 20 years of coating girds with
no problem. It turned out the powder (I make my own solution) I was getting
was contaminated with water. I found this out when I called the supplier
and asked for a bottle from a new lot and all they had was that one lot. So
I ordered from a new source and the problem was resolved. It, of course,
took about 2 months of hair pulling and gnashing of teeth to work through
all this. I hope you can resolve your problem more easily.

Sandra Zane
Sandra F. Zane, EM Tech.
Biol. Dept. UNCC
Charlotte, NC 28223
sfzane-at-unccvm.uncc.edu
Fax (704) 547-3128





From: Tina Carvalho :      tina-at-ahi.pbrc.Hawaii.Edu
Date: Wed, 4 Jan 1995 09:52:46 -1000 (HST)
Subject: clarification of glut-polysacc. query

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Let me clarify my question on the interaction between glutaraldehyde and
polysaccharides, and the fluorescence with confocal microscopy---

As I understand it, the people who asked me about this are actually
rather happy that their specimen (whatever it is) is fluorescing so well
and giving great photos. But they would like to be able to explain it.
I gather they think they don't have all that much protein in their sample
and so would like to explain it away by glut binding to polysaccharides.
Is this plausible? Or can someone come up with another explanation?

Thanks, again, for musing on this!

It's 79 F, clear, sunny, and the surf is up. Happy New Year!

Tina Weatherby Carvalho
Biological EM Facility
University of Hawaii





From: tivol-at-tethys.ph.albany.edu
Date: Wed, 04 Jan 1995 14:59:18 EST
Subject: safety issue - eyes

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Dear Robin,
There is unlikely to be damage to the eye due either to EM use per se
or to radiation--in the first place, eye tissue is not fast-growing like in-
testinal epithelium, and in the second place, the radiation levels should be
low. At our HVEM, for example, there is { 0.25 mr/hr (usually { { 0.25 mr/hr),
and the column is monitored and interlocked to shut off the beam if the level
exceeds 0.75 mr/hr at any of three positions. The most serious eye safety
problem, IMHO, is exposure to the chemicals used in specimen preparation.
Yours,
Bill Tivol




From: Paul Webster :      Paul_Webster-at-QuickMail.Yale.edu
Date: 4 Jan 1995 16:57:08 -0500
Subject: Glut-polysacc.

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Tina,
It sounds like you are fixing plant tissue, which often is autofluorescent.
An easy way to check, of course, is to look at unfixed tissue.
As this is the main point of your question it seems pointless to go too deeply
into gluteraldehyde chemistry. If you are interested, though, I will put you
in contact with an expert ( he wouldn't forgive me for making his name too
public).
Gluteraldehyde reacts very rapidly with amines to form numerous products. The
well known reactions are with primary amines but sulfhydral groups from
cysteine and imidazole side chains of histidine also help in the cross-linking
process. Theoretically, primary amino groups on amino lipids should also
react with gluteraldehyde.
As far as I understand it, there is little chance of gluteraldehyde
cross-linking carbohydrates. That they are retained in fixed material is
probably due to the cross-linking of nearby proteins which hold them in place.
The best visual demonstration I saw was the addition of gluteraldehyde to
homogenates of different tissues that were stirring in beakers. Liver and
striated muscle gelled the instant gluteraldehyde was added. Brain gelled
after a couple of hours and apple leaves were still stirring when we returned
the next day!
Hope this is of help to you.
If it makes you feel better, in New Haven it is dark before 5 pm, there is a
light dusting of snow on the ground and it is cold (below zero).
What's it like in the Twin Cities anyone?








From: _ _ _ _ _ MARK W. LUND _ _ _ _ _ :      LUNDM-at-PHYSC1.BYU.EDU
Date: Wed, 4 Jan 1995 18:09 MST
Subject: SUTW window failures

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The subject of ultra thin window failures is one of extreme interest to me,
since MOXTEK is the largest supplier of these windows. I am afraid that
an informal survey on the Net will give warped data, since usually only
those that have problems respond. We have supplied over 5000 ultra thin
windows. We have a very good idea of failure rates over the first year,
which is our warantee period to the EDX manufacturer. We also have some
visability beyond that, because we often do failure analysis on windows
for EDX manufactures as a service.

The data vary due to the fact that some microscopes are harder on windows
than others. The most common failure in the field is due to particles
striking the window during venting. The second most common failure is
touching the window with fingers, stages, samples. etc. Very rarely
does the window fail in such a manner as to be traced to defects in the
manufacture. All this being said we have data showing over 7 years
mean time before failure from one EDX manufacturer, and a total of 5%
returns over the history of SUTW shipments from another manufacturer.

I would love to get anecdotal information from people that have had
problems with x-ray windows to help us in making the window more reliable.
These windows are by very nature extremely fragile. Only by the
most careful manufacture, testing, and installation could the high
reliability of these windows have been reached.

regards

Mark W. Lund
MOXTEK, Inc.
Orem UT




From: Gardnerjs-at-yvax.byu.edu (gardnerjs)
Date: Wed, 04 Jan 1995 18:40:08 -0700 (MST)
Subject: TEM - Bone Decalcification

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Hi everyone:

We are beginning a new project with bone tissue. Samples are being
prepared for TEM and we have a couple of procedures for decalcification.
We have not found any procedures for detecting the endpoint of the decal.
process.

I would grateful for any assistance regarding decal. endpoint detection and
additional decal. procedures.

Thanks for your help,

John

John S. Gardner
Microscopy Lab
128 WIDB
Brigham Young University
Provo, UT 84602

Phone: 801-378-2202






From: echernof-at-ucs.indiana.edu (Chernoff)
Date: Wed, 4 Jan 1995 21:37:58 -0500
Subject: AFM: paper laminate, chemical contrast

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Some time ago, Dr. Lisa Detter-Hoskin asked about edge wick
experiments on a paper laminate material. She invited "any other
thoughts about evaluating the morphology".

We have found that Scanning Probe/Atomic Force Microscopy yields
interesting information about the surface structure of wood (and
many other) fibers.
Recently in our laboratory we have begun to supplement the
topographic information of AFM by developing methodology for point
chemical analysis. In fact, one of our early successes was in
distinguishing lignin from cellulose on wood pulp fibers.

If this type of information interests you, please contact me.

DON CHERNOFF 317-251-1364
ADVANCED SURFACE MICROSCOPY FAX: 317-254-8690
6009 KNYGHTON RD. E-MAIL: echernof-at-ucs.indiana.edu
INDIANAPOLIS IN 46220 Toll free: 800-374-8557
ASM is an independent analytical service and contract research laboratory.





From: echernof-at-ucs.indiana.edu (Chernoff)
Date: Wed, 4 Jan 1995 21:36:29 -0500
Subject: AFM: NanoScope III height formula

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Gernot Fuchs asked about the NanoScope III height formula, in order
to scale bitmap data for display by the NS3.
I have studied the NS3 data format in detail, in order to create a
software package called "GP3 - Enhancement Software for NanoScope
III" (by the way, this software is available for sale).

Here are some specific answers to Fuchs' questions:
-The factor (Zmax * 2) is used because Zmax = 220V in the data file
parameter list, but the total Z range of the piezo is 440V.
-Fuchs may have neglected an important parameter from the "image
list". In version 2 software, this is "\Z scale". In version 3
software, this is "\Z scale height". It is necessary to supply an
appropriate value for this parameter in the file header in order for
NS3 to display the data correctly. Some experimentation might be
necessary to select the correct value for Fuchs' purpose--I only
considered this problem going in the opposite direction.

DON CHERNOFF 317-251-1364
ADVANCED SURFACE MICROSCOPY FAX: 317-254-8690
6009 KNYGHTON RD. E-MAIL: echernof-at-ucs.indiana.edu
INDIANAPOLIS IN 46220 Toll free: 800-374-8557
ASM is an independent analytical service and contract research laboratory.





From: Hans.Ekwall-at-ah.slu.se (Hasse Ekwall)
Date: Thu, 5 Jan 1995 10:20:34 +0100
Subject: Polychromatic stains for epoxy sections - muscle fibers

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We are trying to classify muscle fibers on semithin sections embedded in
Agar 100 for further analyze in the electron microscope. Normally one uses
toulidine blue as staining but we would like to classify the fibers of
different (type IIA, IIB, I,... etc) at the light microscopic level to be
able to cut out the right fiber for EM.
Is there a staining method for epoxy sections that is useful for this purpose?
There is some polychromatic stains but I cant recall the reference.
Anyone having any ideas?


SWEDISH UNIVERSITY AGRICULTURAL SCIENCES
Hans Ekwall
Dept. Anatomy & Histology
Box 7011, S-750 07 Uppsala, SWEDEN

E-mail Hans.Ekwall-at-ah.slu.se
Voice: +46 18 672141 Telefax: +46 18 672852






From: STUTZ-at-ENH.NIST.GOV
Date: Thu, 05 Jan 1995 07:31:42 -0500 (EST)
Subject:

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subscribe




From: COOK-at-AAEM.AMC.ANL.GOV
Date: Thu, 5 Jan 1995 9:12:08 -0600 (CST)
Subject: Liquid helium stages for TEM

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We are contemplating the purchase of a liuid helium cooling stage for
a TEM with a side-entry goniometer. What companies besides Oxford Instruments
and Gatan manufacture LHe stages? Would those of you who use such stages
please give me your opinions about your equipment? Specifically, what are the
lowest temperatures reached routinely, and what is the degree of difficulty in
using the equipment?

Russell Cook
Electron Microscopy Center for Materials Research
Argonne National Laboratory
Argonne, IL 60439
(708)252-7194




From: loewe-at-uni-bonn.de (Andreas Loewe)
Date: Mon, 5 Dec 1994 13:53:25 +0100
Subject: Who is working on amorphous inorganic states?

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X-Sender: UNC900-at-ibm.rhrz.uni-bonn.de
Message-Id: {v01510100ab08bc2a09fa-at-[131.220.128.28]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Hi all!

Does anybody know about a newsgroup or a list dealing with amorphous
inorganic states???
Or is anybody involved by himself/herself???

Any comments highly welcomed!!!!

Nicole Wartner






From: Jean-Luc Rouviere :      rouvier-at-drfmc.ceng.cea.fr
Date: Thu, 5 Jan 1995 16:12:07 --100
Subject: Who is working on amorphous inorganic states?

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Hi everyone,

I am about to buy a dye sublimation printer and hesitate between the 3 following products.
1) Tektronix 440 (that has replaced phaser IISDX)
2) Kodak XLS8600 (that has replaced the kodak color Ease)
3) Codonics NP1600 (based on the kodak XLS8600)
Our electron microscopy images are all made of grey levels and I would like to use the new monochrome ribbons that are cheaper than the coloured one.
More precisely, my idea would be to work with monochrome ribbons most of the times and from time to time to use coloured ribbons.
* The Tektronix seems too be well adapted to do this because it has a special ribbon tray.
However, its black ribbons make green-grey pictures
* The Kodak monochrome ribbon gives more grey pictures, but the exchange of the ribbon could be more precarious as there is no special ribbon tray.
* Codonics will sell the monochrome ribbons in France only in April ? (Although the kodak monochrome ribbons that should be the same are already available ?)

Has anyone any comments or experience on that ?
Has anyone found problem running one of the three previous printers (especially the Codonics with its special image conversion software) ?

Thank you in advance





From: Fermin, Cesar :      Fermin.Cesar-at-tmc.tulane.edu
Date: 05 Jan 1995 11:58:47 -0600
Subject: Processor & grid stainer cheap

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We want to sell,l and will let go as pair to best offer a 1) TEM Lynx (Leica)
Tissue Processor and a 2) Grid Leica Ultra Stainer. If interested, and your
offer is serious (that is not a couple hundred bucks) I will compile offers for
a week and then contact the winner and runner up. Both items were purchased
new in 1990 for about $15,000 and were hardly used, mainly because the work
load is not sifficien and when use we end up wasting solutions. I will include
some solutions with instruments.

Please respond directly to me {Fermin-at-TMC.Tulane.edu} , NOT to the user list.

************************************************************
*Cesar D. Fermin, Ph.D \|*|/ Fax (504) 587-7389 *
*Tulane Medical School /|*|\ Answ. Mach.(504) 584-2618 *
*Pathology/SL79 \|*|/ Secretary (504) 584-2436 *
*New Orleans, La 70 112 /|*|\ Lab (504) 5841 *
*Fermin-at-TMC.Tulane.edu -} Director of Morphological Services*
************************************************************




From: hmeekes-at-biosci.mbp.missouri.edu (Herman Meekes)
Date: Thu, 5 Jan 1995 11:13:45 -0600
Subject: Re: Polychromatic stains for epoxy sections - muscle fibers

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On January 5 1995 Hans Ekwall wrote:

} We are trying to classify muscle fibers on semithin sections embedded in
} Agar 100 for further analyze in the electron microscope. Normally one uses
} toulidine blue as staining but we would like to classify the fibers of
} different (type IIA, IIB, I,... etc) at the light microscopic level to be
} able to cut out the right fiber for EM.
} Is there a staining method for epoxy sections that is useful for this purpose?
} There is some polychromatic stains but I cant recall the reference.
} Anyone having any ideas?
}
}
} SWEDISH UNIVERSITY AGRICULTURAL SCIENCES
} Hans Ekwall
} Dept. Anatomy & Histology
} Box 7011, S-750 07 Uppsala, SWEDEN
}
} E-mail Hans.Ekwall-at-ah.slu.se
} Voice: +46 18 672141 Telefax: +46 18 672852
}


A relatively simple and fast procedure for polychromatic staining of epoxy
sections has been published by J.Tolivia et al in Histochemistry 101:
51-55. I have not used it myself. Ask me for details if this reference is
not readily available to you.

Greetings!


Herman Meekes
Biological Sciences ______________ ______________
University of Missouri ---__ \ / __---
109 Tucker Hall ------__\---/__------
Columbia, MO. 65211 \( )/
Tel: 314-882-0171 V
Fax: 314-882-0123 / \
e-mail: hmeekes-at-biosci.mbp.missouri.edu /___\








From: Chris Frethem (CBN) :      frethem-at-lenti.med.umn.edu
Date: Thu, 5 Jan 1995 10:49:04 -0600 (CST)
Subject: Re: Glut-polysacc.

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I can't contribute to the glut-polysacc. discussion, but since you asked
for a weather report... a very mild winter here so far, but went skating
last night under the stars; temp -6 F, wind chill below -20. Cold nose,
cold toes, but had the ice all to myself. Warm breezes are nice, but
Winter's great too. Need more snow, though. Thanks for the review of glut
reactivity.



=======================================================================
Chris Frethem (612)624-4652 (voice)
Cell Biology & Neuroanatomy (612)624-8118 (FAX)
U of MN, Minneapolis e-mail: frethem-at-lenti.med.umn.edu



On 4 Jan 1995, Paul Webster wrote:

} Tina,
} It sounds like you are fixing plant tissue, which often is autofluorescent.
} An easy way to check, of course, is to look at unfixed tissue.
} As this is the main point of your question it seems pointless to go too deeply
} into gluteraldehyde chemistry. If you are interested, though, I will put you
} in contact with an expert ( he wouldn't forgive me for making his name too
} public).
} Gluteraldehyde reacts very rapidly with amines to form numerous products. The
} well known reactions are with primary amines but sulfhydral groups from
} cysteine and imidazole side chains of histidine also help in the cross-linking
} process. Theoretically, primary amino groups on amino lipids should also
} react with gluteraldehyde.
} As far as I understand it, there is little chance of gluteraldehyde
} cross-linking carbohydrates. That they are retained in fixed material is
} probably due to the cross-linking of nearby proteins which hold them in place.
} The best visual demonstration I saw was the addition of gluteraldehyde to
} homogenates of different tissues that were stirring in beakers. Liver and
} striated muscle gelled the instant gluteraldehyde was added. Brain gelled
} after a couple of hours and apple leaves were still stirring when we returned
} the next day!
} Hope this is of help to you.
} If it makes you feel better, in New Haven it is dark before 5 pm, there is a
} light dusting of snow on the ground and it is cold (below zero).
} What's it like in the Twin Cities anyone?
}
}
}
}
}




From: Gib Ahlstrand :      giba-at-puccini.crl.umn.edu
Date: Thu, 5 Jan 1995 14:50:38 -0600
Subject: TEM of hair

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Hair is a difficult tissue to infiltrate and embed. We've been using an
extended infiltration protocol with Embed 812. Cutting thicker than usual
sections (150 nm) gives marginal results. Has anyone worked with various hair
samples lately? We're looking for better infiltration and less wrinkling of the
cortex and medulary areas. We'd also like to get TEM pictures of whole cross
and longitudinal sections. Any ideas???

--

Darryl Krueger
Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
495 Borlaug Hall, St. Paul, MN 55108 (612)625-8249
612-625-9728 FAX, giba-at-puccini.crl.umn.edu






From: Michael Rock :      merock-at-u.washington.edu
Date: Thu, 5 Jan 1995 13:34:54 -0800 (PST)
Subject: Re: Bubbles in formvar coated grids

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Jamie-
0.25% may be a little thin, I am used to using 0.75% formvar
just my 2 cents
-Mike

On Wed, 4 Jan 1995, Jaime Dant wrote:

}
}
}
} 
}
} Quick question!
} I am getting small bubbles in my formvar ciated grids. How do I get rid of
} these bubbles? I am not overtly introducing moisture onto my glass slides
} am I am using EM Sciences prepared 0.25% formvar from a new bottle!!!
}
} I don't want to make a project out of this, so input is appreciated.
}
} Thank you for your time.
}
} Jaime A. Dant
} jaime-at-borcim.wustl.edu
}
} Sorry about the typo, thats formvar coated grids.
}




From: tivol-at-tethys.ph.albany.edu
Date: Thu, 05 Jan 1995 16:54:04 EST
Subject: RE: safety issue - eyes

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John Mardinly writes:


Just because eye tissue is not fast growing, and there is no exaggerated risk
of tumors, don't neglect the possibility of cataracts. The susceptibility
varies with individuals, but radiation is a definite risk factor.
--John Mardinly
Intel Materials Tech.
Dear John,
It is true that radiation can be a risk factor, and it should be mon-
itored for an EM instrument. In our case, with { 0.25 mr/hr, a full shift's
use of the HVEM (assuming that the beam is only on 50% of the time) will de-
liver { 1 mr. Over the course of a year, this adds up to { 500 mr, the limit
for non-radiation-workers. If other EM's produce as little radiation as the
HVEM, the exposures will also be below the allowed limit. Of course, expos-
ure to any radiation should be kept to "as low as reasonably achievable", in
the words of the NCRP. I would still guess that there are many other sources
of eye damage more significant than radiation exposure from an EM.
Yours,
Bill Tivol




From: Paul Webster :      Paul_Webster-at-QuickMail.Yale.edu
Date: 5 Jan 1995 17:08:40 -0500
Subject: Shark-TEM

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Message-ID: {n1422796673.73692-at-QuickMail.Yale.edu}

Fixing shark tissue shouldn't be too difficult should it? I used 4%
formaldehyde (from paraformaldehyde, in 100 mM PO4 buffer, pH 7.4) which
works well for MDCK cells but swelled up the shark cells. My guess is the
osmolarity is too low and needs to be closer to 1000mOm. Does anyone have any
advice and/or recipes?
I use this fixative to prepare cells for cryosection immunocytochemistry so
that I can do light and electron microscopy on the same tissue pieces (yes 4%
FA for EM doe work). I have no objection to using gluteraldehyde, acrolein or
any other legal substance if it works.

Thank you to all who responded to the weather question.
Brief summary:
Colder than here in Minneapolis and Madison (wind chill -20F, quelle
surprise).
Wonderful in Sidney, Australia (how is the surf?),
and only two more weeks before they get to see the sun again in Tromso,
Norway.
Thanks in advance,
Paul Webster,
Yale School of Medicine.





From: SALLY STOWE :      STOWE-at-rsbs-central.anu.edu.au
Date: Fri, 6 Jan 1995 11:23:40 EST10
Subject: Quartz crystals for Balzers thickness monitor

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We have a Balzers freeze-fracture 301, not exactly the latest model
but working very well, and have struck a problem with the supply of
crystals for the thickness monitor, which is the model QSK 113.
Balzers evidently no longer supply them -- has anyone else had this
problem and found another source?

Thanks,
Sally
----------------------------------------------------------------------
Sally Stowe Australian National Univ.
Facility Coordinator Canberra, AUSTRALIA
ANU Electron Microscopy Unit Ph 61 6 249 2743
RSBS, Box 475
Email stowe-at-rsbs-central.anu.edu.au FAX 61 6 249 4891

------------------------------------- --------------------------------
-





From: RETEP-at-anat.uct.ac.za
Date: 6 Jan 95 09:56:38 SAST-2
Subject: Re: Hair

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Message-ID: {MAILQUEUE-101.950106095638.480-at-anat.uct.ac.za}
To: microscopy-at-aaem.amc.anl.gov

New Year greetings to everyone,

Weather in Cape Town Hot and windy typical for this time of year. For
the surfers out there SURF IS UP.

Getting to the query regarding Hair.

We routinely look at hair samples (albino and normal). We use a
standard Spurr resin which gives quite a good result. I do extend
the infiltration time by not putting in accelerator and allowing the
resin to infiltrate overnight at 40 degrees C. I have also obtained
reasonable results with Molleneur's Epon / Araldite mixture slightly
modified with the same extended time in the oven for the initial
infiltration. Infiltration with the accelerator is also as long as
possible at 40 degrees before embedding when using the Epon /
Araldite mix.

Using this method I can get good cross and longtitudinal sections.

Hope you have some success with these.

Peter
_______________________________________________________________


-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
-at- -at-
-at- Peter D. G. Richards -at-
-at- Dept Anatomy and Cell Biology -at-
-at- UCT Medical School -at-
-at- Observatory -at-
-at- 7925 -at-
-at- RSA -at-
-at- Tel: 021-406 6285. -at-
-at- Internet: retep-at-anat.uct.ac.za -at-
-at- -at-
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-




From: STUTZ-at-ENH.NIST.GOV
Date: Fri, 06 Jan 1995 08:41:29 -0500 (EST)
Subject: Needed! Beam-stable embedding material for SEM

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I am working on imaging polished cross sections of portland cement particles
by BE and XR imaging in the SEM. The cement powder is mixed with a resin
(L.R. White) and the resin is cured. Cement particles are typically finer
than 100 microns. As about half of the polished section is resin I am
having difficulty with the operating conditions necessary (12kV, 5nA) burning
the resin. Does anyone have suggestions for a better embedding medium? The
ideal material would be stable under the beam, not allow plucking of fine
particles during polishing, be fairly hard, and not be hazardous to handle
or for disposal. Thank you in advance for your suggestions.




From: zhang :      zhang-at-macgw1.crd.ge.com
Date: 6 Jan 1995 08:42:41 U
Subject: subscribe

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Message-Id: {n1422740675.42601-at-macgw1.crd.ge.com}

subscribe microscopy




From: M.V. Parthasarathy :      mvp2-at-cornell.edu
Date: Fri, 6 Jan 1995 11:06:15 -0400 (EDT)
Subject: RE: Quartz crystals for Balzers thickness monitor

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X-NUPop-Charset: English

In message Fri, 6 Jan 1995 06:23:40 -0500,
"SALLY STOWE" {STOWE-at-rsbs-central.anu.edu.au} writes:

} We have a Balzers freeze-fracture 301, not exactly the latest model
} but working very well, and have struck a problem with the supply of
} crystals for the thickness monitor, which is the model QSK 113.
} Balzers evidently no longer supply them -- has anyone else had this
} problem and found another source?
}
} Thanks,
} Sally
} ----------------------------------------------------------------------
} Sally Stowe Australian National Univ.
} Facility Coordinator Canberra, AUSTRALIA
} ANU Electron Microscopy Unit Ph 61 6 249 2743
} RSBS, Box 475
} Email stowe-at-rsbs-central.anu.edu.au FAX 61 6 249 4891
}
} ------------------------------------- --------------------------------
} -
}
***************

I have even an even older Balzers BA360 that I use for teaching. If you
have not thrown away the used quartz crystals that had stopped functioning due
to heavy deposition of platinum/carbon on them, you can remove the deposits
by carefully "rubbing" them out with an unused pencil eraser (the one on
the back of pencils work fine) and reuse them!

*************************************************************************
M.V. Parthasarathy
Professor of Plant Biology
Section of Plant Biology
228 Plant Science Building
Cornell University, Ithaca, NY 14850
Telephone: (607) 255-1734
Fax: (607) 255-5407
E-mail: mvp2-at-cornell.edu
***********************************************************************




From: Michael Rock :      merock-at-u.washington.edu
Date: Fri, 6 Jan 1995 09:19:14 -0800 (PST)
Subject: Re: Needed! Beam-stable embedding material for SEM

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You might try Streuers "EPOFIX" resin, it is avail. through most EM
vendors, it cures very hard, and is recomended for non-biological materials.
-Mike


On Fri, 6 Jan 1995 STUTZ-at-ENH.NIST.GOV wrote:

} I am working on imaging polished cross sections of portland cement particles
} by BE and XR imaging in the SEM. The cement powder is mixed with a resin
} (L.R. White) and the resin is cured. Cement particles are typically finer
} than 100 microns. As about half of the polished section is resin I am
} having difficulty with the operating conditions necessary (12kV, 5nA) burning
} the resin. Does anyone have suggestions for a better embedding medium? The
} ideal material would be stable under the beam, not allow plucking of fine
} particles during polishing, be fairly hard, and not be hazardous to handle
} or for disposal. Thank you in advance for your suggestions.
}




From: STUTZ
Date: Friday, January 06, 1995 08:41AM
Subject: Needed! Beam-stable embedding material for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am working on imaging polished cross sections of portland cement particles
by BE and XR imaging in the SEM. The cement powder is mixed with a resin
(L.R. White) and the resin is cured. Cement particles are typically finer
than 100 microns. As about half of the polished section is resin I am
having difficulty with the operating conditions necessary (12kV, 5nA)
burning
the resin. Does anyone have suggestions for a better embedding medium? The
ideal material would be stable under the beam, not allow plucking of fine
particles during polishing, be fairly hard, and not be hazardous to handle
or for disposal. Thank you in advance for your suggestions.




From: South Bay Technology :      73531.1344-at-compuserve.com
Date: 06 Jan 95 16:48:51 EST
Subject: Needed! Beam-stable embedding material for SEM

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We have several materials that are used extensively in SEM appliactions that you
may want to consider:

EpoxiMet - Epoxy
AcryliMet - Acrylic
PolyMet - Polyester

These vary in hardness, cure rate, clarity etc. so you can select the one that
best fits your needs. If you provide your name and mailing address, I'll be
please to send you specifications on these materials along with prices. I'll
also send information on our other materials for cutting, grinding, polishing
etc for metallography and electron microscopy.

Thank you!

David Henriks
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673

TEL: 714-492-2600
800-728-2233
FAX: 714-492-1499





From: _ _ _ _ _ MARK W. LUND _ _ _ _ _ :      LUNDM-at-PHYSC1.BYU.EDU
Date: Fri, 6 Jan 1995 16:48 MST
Subject: SUTW window failures--correction

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In my previous post I mentioned that we have supplied over 5000 ultra
thin windows. This should have been 'over 4000 ultra thin windows.'
It just seems like like a lot more....:)
With my integrity back intact,
Mark Lund
MOXTEK, Inc
Orem UT




From: HAHNP-at-JEFLIN.TJU.EDU
Date: Fri, 6 Jan 1995 21:12:19 -0500 (EST)
Subject: dye-sub printer evaulation

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} I am about to buy a dye sublimation printer and hesitate between the 3
following} products.
} 1) Tektronix 440 (that has replaced phaser IISDX)
} 2) Kodak XLS8600 (that has replaced the kodak color Ease)
} 3) Codonics NP1600 (based on the kodak XLS8600)

We did a month long evaluation of several dye-sub printers for our facility,
and finally received shipment of the Seiko ColorpointPSF dye-sub last week.
Our original budget was in the $7,000 range, however, once the ethernet
option, memory, extral printer trays, etc were factored in, the printer camein
at just over $9,000.

For our evaluation we had color confocal images as well as b&w scanned gels.
We sent out the files to the various resellers for sample prints. To do an
apples to apples comparison. Incidentally, Codonics was the only
organization with an ablitiy to ftp image files, however, their connection
appeared to be a SLIP connection requiring a tremendous amount of patience
when ftp ing.

We received samples from all three manufactures as well as Seiko. Now, we
do know from previous postings that the quality of the printer driver often
determines the quality of the hardcopy prints. There are a tremendous
number of settings when printing to the dye-sub including, linear inks,
color ramps, etc, things catering more to the pubishing industry. However, as
we did not have the time to get into all that, we just took whatever each
manufacture printed at face value. The bulk of our printing needs are gels and
images from a phosphorimager.

The interesting thing is that although the Codonics was the pricier printer
with rave reviews from almost everyone, we found the Seiko noticeably better
for color. For b&w, the Seiko exhibited truely dark blacks. Tecktronix &
Condonics prints had either a brownish or greenish tint. The Seiko also has
the added advantage of printing on cheaper thermal paper. In evaluating
dye-sub to purchase try to get sample prints of your own image files. Codonics
sells their printer exclusively while the other manufacturers use resellers.

Feel free to email me questions directly regarding our dye-sub pruchase. There
is a reseller based in New York who was tremendously helpful in obtaining the
various sample prints. Our core facility is seriously considering the purchase
of another dye-sub, the Itochu Pictograph, the Cadillac (or Lexus) of dye-subs.



._____________________
| Peter J. Hahn
| -------------
| Thomas Jefferson University
Jefferson Cancer Institute Confocal Facility
JCI-BLSB-915
233 South 10th Street
Philadelphia, PA. 19107
tel : 215-955-4770 |
fax : 215-923-1098 |
hahnp-at-jeflin.tju.edu |
-------------+-----------------+----+------+





From: MCMOULDK-at-usthk.ust.hk
Date: 07 Jan 1995 11:30:37 +0800
Subject: WORKSHOP IN BEAUTIFUL HONG KONG

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**************************************************************************


WORKSHOP ON ELECTRON MICROSCOPY OF MATERIALS
(organizers: Gareth Thomas & David Barber)

A workshop on Electron Microscopy will be held at the Hong Kong University of
Science and Technology between the 19th and 25th of February 1995.
The aim is to present the principles of scanning and transmission microscopy,
diffraction and microanalysis, specimen preparation and applications.
In addition to lectures there will be daily lab sessions on microscopy,
analytical methods and specimen preparation techniques.

The HKUST e.m. facility is part of a new well-equipped research centre, with
several scanning and transmission microscopes from Philips and JEOL available
for demonstration and hands-on use. The workshop is being supported by many
of the major manufactures including JEOL, Philips, Gatan, Oxford Instruments,
and South Bay Technology. Various ancillary pieces of equipment will be
for use by the participants and specialists from the companies will be
on hand to discuss any problems.

In addition, once the week of lectures and hands-on practice is finished,
you can relax with a free trip in a motorised Chinese Junk around beautiful
Hong Kong!

As the Workshop is limited in numbers, please be prompt in applying.

For further information and registration, please contact:

Miss Alice Yuen,
HKUST RandD Corporation Ltd,
The Hong Kong University of Science and Technology,
Clear Water Bay,
Kowloon,
Hong Kong.

Telephone: (852) 2-358-7916, (852) 2-358-7917
Fax: (852) 2-358-2759, (852) 2-358-1493
E-mail: TTALICE-at-usthk.ust.hk


************************************************************************




From: Dr. R. Beanland :      beanland-at-liverpool.ac.uk
Date: Mon, 9 Jan 1995 09:38:34 +0000 (GMT)
Subject: TEM Epoxies

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Hello Netters,
Since there is a discussion on epoxy for SEM, I thought I'd
broaden the discussion a bit. Are there any epoxies available for making
TEM cross-sections (from semiconductor materials in this case) that cure
quickly and are stable during ion milling and under the electron beam?
I currently use 'Devcon' - a 5 minute epoxy - but it tends to evaporate
under the ion beam and isn't particularly stable in the electron beam. The
best glue I've ever used is good old Araldite, which you can buy at any
hardware store, but takes a long time to cure. Araldite 'rapid' seems to
have the same problems as Devcon. What else is available?


Richard Beanland
Dept. of Mat. Sci. and Eng.
University of Liverpool,
P.O. Box 147,
Liverpool L69 3BX
England.




From: sbarlow-at-sunstroke.sdsu.edu (Steve Barlow)
Date: Mon, 9 Jan 1995 10:27:51 -0800
Subject: nerve fiber em stains

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One of my researchers wants to identify nerve fibers in whole mounts of
Drosophila muscle preparations. Any suggestions for selectively labeling
nerves? References from the 1980s use cobalt filling and/or silver
nitrate, but note that muscle fiber will also take up the stain.

We would like to use both TEM and SEM. Backscatter mode for silver
impregnated nerves looked interesting, but not if both nerve and insect
muscle take up the label.

suggestions in this regard would be appreciated. Thanks in advance

steve

----------------------------------------------------------
Dr. Steven Barlow
EM Facility/Biology Dept.
San Diego State University
San Diego CA 92182-0057
phone: (619) 594-4523
fax: (619) 594-5676
email to sbarlow-at-sunstroke.sdsu.edu





From: mrdunlap-at-ucdavis.edu (Michael Dunlap)
Date: Mon, 9 Jan 1995 11:33:45 -0800
Subject: SEM of bone

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Message-Id: {m0rROpQ-00011LC-at-pegasus.cc.ucf.edu}

Happy New Year to all -

We are beginning a project where we will be looking at the structure of
different regions of several bones that are subject to stress fractures.
We are planning to bleach the samples to remove the soft tissue and then
use acetone to remove any fat. We were wondering if it is necessary to
Critical Point Dry the samples or just allow air drying. Are there any
known changes that occur in bone material when allowed to air dry? In
addition, is fixation necessary when looking at just bone? I would
appreciate any comments. Thanks

Mike


---------------------------------------------------------------------------
| Michael Dunlap | lab (916) 752-0284 |
| Facility For Advanced Instrumentation | fax (510) 422-2282 |
| University of California | mrdunlap-at-ucdavis.edu |
| Davis CA, 95616 | |
===========================================================================






From: Glen Macdonald :      glenmac-at-u.washington.edu
Date: Mon, 9 Jan 1995 11:58:29 -0800 (PST)
Subject: Re: TEM - Bone Decalcification

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X-Sender: glenmac-at-homer07.u.washington.edu

There are several tests, which I found in the book: "The
Preparation of Decalcified Sections" by Edward B. Brain, pub. by Charles C.
Thomas, 1966. Precipitation with calcium oxalate and calcein
fluorescence seem to be the best alternatives to pin pricking or
X-ray. Techniques for oxalate and calcein appear in pp. 138-141. I use
oxalate pptn.

Glen MacDonald
Hearing Development Laboratories RL-30
University of Washington
Seattle, WA 98195
(206)543-8360
glenmac-at-u.washington.edu


On Wed, 4 Jan 1995, gardnerjs wrote:

} Hi everyone:
}
} We are beginning a new project with bone tissue. Samples are being
} prepared for TEM and we have a couple of procedures for decalcification.
} We have not found any procedures for detecting the endpoint of the decal.
} process.
}
} I would grateful for any assistance regarding decal. endpoint detection and
} additional decal. procedures.
}
} Thanks for your help,
}
} John
}
} John S. Gardner
} Microscopy Lab
} 128 WIDB
} Brigham Young University
} Provo, UT 84602
}
} Phone: 801-378-2202
}
}
}




From: mrdunlap-at-ucdavis.edu (Michael Dunlap)
Date: Mon, 9 Jan 1995 13:34:27 -0800
Subject: SEM of bone

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Happy New Year to all -

We are beginning a project where we will be looking at the structure of
different regions of several bones that are subject to stress fractures.
We are planning to bleach the samples to remove the soft tissue and then
use acetone to remove any fat. We were wondering if it is necessary to
Critical Point Dry the samples or just allow air drying. Are there any
known changes that occur in bone material when allowed to air dry? In
addition, is fixation necessary when looking at just bone? I would
appreciate any comments. Thanks

Mike

---------------------------------------------------------------------------
| Michael Dunlap | lab (916) 752-0284 |
| Facility For Advanced Instrumentation | fax (510) 422-2282 |
| University of California | mrdunlap-at-ucdavis.edu |
| Davis CA, 95616 | |
===========================================================================






From: Marc Brande :      brande-at-sdsc.edu
Date: Mon, 9 Jan 1995 14:28:01 -0800 (PST)
Subject: Re: nerve fiber em stains

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See the many fluorescent markers for neurons made by Molecular Probes,
Eugene, Oregon. They have a free very informative voluminous catalog and
reference guide to all their hundreds of fluorescent probes.

Marc C. Brande, M.S. SD3D Email List:3D Imaging
San Diego 3D Imaging Group To subscribe/unsubscribe:listserv-at-bobcat.etsu.edu
3840 Camino Lindo To post a message:sd3d-at-bobcat.etsu.edu
San Diego, CA 92122 Email:BRANDE-at-SDSC.EDU Voice:619-587-4830


On Mon, 9 Jan 1995, Steve Barlow wrote:

} One of my researchers wants to identify nerve fibers in whole mounts of
} Drosophila muscle preparations. Any suggestions for selectively labeling
} nerves? References from the 1980s use cobalt filling and/or silver
} nitrate, but note that muscle fiber will also take up the stain.
}
} We would like to use both TEM and SEM. Backscatter mode for silver
} impregnated nerves looked interesting, but not if both nerve and insect
} muscle take up the label.
}
} suggestions in this regard would be appreciated. Thanks in advance
}
} steve
}
} ----------------------------------------------------------
} Dr. Steven Barlow
} EM Facility/Biology Dept.
} San Diego State University
} San Diego CA 92182-0057
} phone: (619) 594-4523
} fax: (619) 594-5676
} email to sbarlow-at-sunstroke.sdsu.edu
}






From: Alan Pooley :      pooley-at-ahab.rutgers.edu
Date: Mon, 9 Jan 1995 17:22:57 -0500
Subject: film recorder for sem & betamax images

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I know others were discussing this but I have not kept up:
What do people consider to be the best but not hugely expensive film
recorder for recording digital images to 4 * 5 film at about 2000 lines
resolution?
Details: we want to make digital composite images of up to 16 objects
(bivalve shells) each digitally recorded on an ETEC SEM at about 2000 line
original resolution (we do not yet have this digitizer, wanting to get the
recorder specifications determined first, we are aiming toward the 4PI
company, any experience with them)
We also want to print color images, (probably to 35 mm film?) from beta max 3/4 inch tape (we do not yet have the beta tape reader but think we have a knowledgeable source (any recommendations welcome) We want the very highest resolution
for both color and spatial detail but realize that beta is only about 700
lines original (recorded on beta tape from 3 chip high resolution
cameras)
We make the b/w composite SEM images by enlarging 4 * 5 negatives to the
correct size, cutting out the images, pasting to black background special
paper, make a tmax or plus x 4 * 5 photographic negative, also photograph
text letraset independanly, combine the negative and make prints in the darkroom, no digital component.
We want to get rid of all the darkreoom except the final prints from a
negative (we need multiple copies) and also to be able to do the color
images from a beta tape through a macintosh via photoshop (we have photoshop
and a mac that probably does not have enough memory, we plan to upgrade
when we know what input and output requirements are needed.
Sorry abou the length but any help would be appreciated. The old
XL 7000 kodak film recorder was recommended by a user but is no longer mad
I like the Harris b/w dry silver printer but we need negatives to make
multiple copies and it does not do color AND? it may not be high enough
resolution for the composite pictures? any comments?
If I get good returns of info, I will post a summary to the group.
Alan Pooley Marine Science SEM lab rutgers univ 908 932 8959 x 225
pooley-at-ahab.rutgers.edu





From: {JMARDINLY-at-MATTEC.intel.com}:ddn:wpafb
Date: 1-9-95 4:13pm
Subject: RE:EPOXIES

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Message-Id: {9501100024.AA15042-at-riker.ml.wpafb.af.mil}
To: WALCKSD:ml:wpafb, SCHELTFJ:ml:wpafb
Subj: EPOXIES
Orig-Author: {"John Mardinly, 5-2346, Page 322-6490, SC2-24" {JMARDINLY-at-MATTEC.intel.com} }:ddn:wpafb
-----------------------------------------------------------
Another epoxy that is excellent and particularly suited for rough surfaces is
Varian Torr-Seal. The stuff is made to patch high vacuum systems that will be
baked up to 250C. It is strong and extremely resistant to beam damage.

John Mardinly
Intel Materials Technolo





From: {beanland-at-liverpool.ac.uk}:ddn:wpafb
Date: 1-9-95 7:59am
Subject: TEM Epoxies

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Message-Id: {9501100023.AA15025-at-riker.ml.wpafb.af.mil}
To: WALCKSD:ml:wpafb, SCHELTFJ:ml:wpafb
Subj: TEM Epoxies
Orig-Author: {"Dr. R. Beanland" {beanland-at-liverpool.ac.uk} }:ddn:wpafb
-----------------------------------------------------------
Hello Netters,
Since there is a discussion on epoxy for SEM, I thought I'd
broaden the discussion a bit. Are there any epoxies available for making
TEM cross-sections (from semiconductor materials in this case) that cure
quickly and are stable during ion milling and under the electron beam?
I currently use 'Devcon' - a 5 minute epoxy - but it tends to evaporate
under the ion beam and isn't particularly stable in the electron beam. The
best glue I've ever used is good old Araldite, which you can buy at any
hardware store, but takes a long time to cure. Araldite 'rapid' seems to
have the same problems as Devcon. What else is available?


Richard Beanland
Dept. of Mat. Sci. and Eng.
University of Liverpool,
P.O. Box 147,
Liverpool L69 3BX
Englan








From: Romuald Wroblewski onkpat :      Romuald.Wroblewski-at-onkpat.ki.se
Date: Tue, 10 Jan 1995 13:09:26 +0200 (METDST)
Subject: PTA-stain for EM

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Happy New Year

I am intending to stain epoxy sections of kidney biopsies for
electron microscopy and am in need of a good receipe for a PTA staining
method.

-------------------------
Romuald Wroblewski, Ph.D.
Associate Professor
Department of Pathology
Karolinska Institute
voice/fax:+46-8-7293597
-------------------------





From: watkinv :      watkinv-at-macgw1.crd.ge.com
Date: 10 Jan 1995 08:35:12 U
Subject:

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Subscribe Microscopy




From: O=PRDSMTP; DDA.TYPE=RFC-822; DDA.VALUE=romwro(a)ki.se :      romwro-at-ki.se
Date: Tue, 10 Jan 1995 08:16:56 -0600
Subject: PTA-stain for EM

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I am intending to stain epoxy sections of kidney biopsies for
electron microscopy and am in need of a good receipe for a PTA staining
method.

-------------------------
Romuald Wroblewski, Ph.D.
Associate Professor
Department of Pathology
Karolinska Institute
voice/fax:+46-8-7293597
-------------------------





From: watkinv :      watkinv-at-macgw1.crd.ge.com
Date: Tue, 10 Jan 1995 10:34:46 -0600
Subject: Bone prep for SEM

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From: South Bay Technology :      73531.1344-at-compuserve.com
Date: 10 Jan 95 12:24:13 EST
Subject: Epoxies-where to purchase

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To all of thise interested in where to buy Epotek 353 ND:

You can get Epotek 353 ND from:

Epoxy Technology
14 Fortune Drive
Billerica, MA 01821

TEL 800-227-2201
FAX: 508-663-9782

You can also buy it from us in smaller quantities - albeit a higher price!

P/N 0714-010 price: $50/8 oz kit

The only advantage in buying from us is that you can combine it with your other
supply orders and we also can ship it to you the same day. Otherwise, you can
get a lot more for your money buying it direct from Epoxy Technology.

I hope this helps!

Best regards-

David Henriks
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673

TEL: 800-728-2233
FAX: 714-492-1499





From: ROBERT K. NAUMAN :      RKN001-at-DENTAL3.AB.UMD.EDU
Date: Tue, 10 Jan 1995 14:57:58 EST
Subject: Source for Xenon lamps

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Can anyone provide me with a source for a new 450 Watt Xenon Lamp?
Thanks!




Bob Nauman
Department of Microbiology
Univ. MD Dental School
Baltimore, MD 21201




From: lporter-at-goodyear.com (LE Porter)
Date: Tue, 10 Jan 1995 12:41:34 -0500
Subject: Microscopy lab plans

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Message-Id: {m0rRnA6-00011oC-at-pegasus.cc.ucf.edu}

We are finalizing plans for a new central microscopy laboratory. In part,
these plans are modeled after those furnished by R A Harris (University of
California, Davis) where areas are compartmentalized i.e. cryo microtoming
in separate room, etc). For general health reasons we have restricted
solvent usage to one room within and isolated from this larger room. We
are experiencing some resistance to this concept. Can any of you lend some
support to this concept? Or tell us why it is not a good idea. Please
contact me via email, fax or phone with your welcome comments. As ever,
thanks in advance for your comments.

L E Porter Phone (216) 796-1620
Head of Microscopy Fax (216) 796-3304
The Goodyear Tire & Rubber Company EMail LPORTER-at-GOODYEAR.COM
Dept 415A
142 Goodyear Blvd
Akron, OH 44305
USA







From: South Bay Technology :      73531.1344-at-compuserve.com
Date: Tue, 10 Jan 1995 12:52:29 -0600
Subject: Epoxies-where to purchase

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You can get Epotek 353 ND from:

Epoxy Technology
14 Fortune Drive
Billerica, MA 01821

TEL 800-227-2201
FAX: 508-663-9782

You can also buy it from us in smaller quantities - albeit a higher price!

P/N 0714-010 price: $50/8 oz kit

The only advantage in buying from us is that you can combine it with your othe
r
supply orders and we also can ship it to you the same day. Otherwise, you can
get a lot more for your money buying it direct from Epoxy Technology.

I hope this helps!

Best regards-

David Henriks
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673

TEL: 800-728-2233
FAX: 714-492-1499





From: Heuer Jim P. :      HeuerJ-at-vncpo1.ne.ge.com
Date: Tue, 10 Jan 1995 13:20:58 -0600
Subject: Resins for XTEM

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I think I'm jumping into the middle of a conversation, but here are my two
cents. I've used Torr-Seal for making cross sections in the past and had
reasonably good luck. It takes about 24 hrs. to cure and stands up well in
the ion mill; I, however, am not sure about the particle issue mentioned.
My sample was well characterized before XTEM.

I still like LR White Hard Grade resin for microtoming particulate material
and free standing optical thin films for XTEM. My samples are typically
ceramics and metals. LR White is stable in the electron beam assuming it is
C-coated and can be cured very quickly with an accelerator or in an oven.
Must be mindful of potential brittleness.

Jim Heuer
GE
(510) 862-4501
heuerj-at-vncpo1.ne.ge.com





From: Heuer Jim P. :      HeuerJ-at-vncpo1.ne.ge.com
Date: Tue, 10 Jan 1995 13:26:10 -0600
Subject: Resins for XTEM

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I think I'm jumping into the middle of a conversation, but here are my two
cents. I've used Torr-Seal for making cross sections in the past and had
reasonably good luck. It takes about 24 hrs. to cure and stands up well in
the ion mill; I, however, am not sure about the particle issue mentioned.
My sample was well characterized before XTEM.

I still like LR White Hard Grade resin for microtoming particulate material
and free standing optical thin films for XTEM. My samples are typically
ceramics and metals. LR White is stable in the electron beam assuming it is
C-coated and can be cured very quickly with an accelerator or in an oven.
Must be mindful of potential brittleness.

Jim Heuer
GE
(510) 862-4501
heuerj-at-vncpo1.ne.ge.com





From: Daniel L. Callahan :      dlc-at-owlnet.rice.edu
Date: Tue, 10 Jan 1995 15:20:11 -0600 (CST)
Subject: cross-sectioning

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We are interested in cross-sectioning devices on silicon with a thin
layer of polyimide. This is primarily for optical or SEM imaging, not
TEM. The polyimide layer is on the order of 10 micrometers thick (and
possibly an order of magnitude higher). My limited microtomy experience
has been that cleaving the polymer introduces substantial dimensional
distortion. Does anyone have a good idea for this? We are considering
trying a "standard" mechanical polish but have serious reservations
because of the polymer. We also know it can be done, having seen good
pictures (lacking, of course, specimen prep information).

Any suggestions will be welcome.

Daniel L. Callahan
Department of Mechanical Engg. and Materials Science
Rice University
dlc-at-owlnet.rice.edu




From: j.chalcrof :      chalcrof-at-alf.biochem.mpg.de
Date: Tue, 10 Jan 1995 18:07:17 +0100 (GMT)
Subject: Re: PTA-stain for EM

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}
} Happy New Year
}
} I am intending to stain epoxy sections of kidney biopsies for
} electron microscopy and am in need of a good receipe for a PTA staining
} method.
}
} -------------------------
} Romuald Wroblewski, Ph.D.
} Associate Professor
} Department of Pathology
} Karolinska Institute
} voice/fax:+46-8-7293597
} -------------------------
}
}
Hi Romuald,
I think you should try a modification of Stempak & Ward 's (1964)
receipt which involves a methanolic solution of Uranyl Acetate.
Just replace the UA by PTA and carefully follow their method, which
is described in J. Cell Biol. 22 pp. 697-701. I have used this with
success for staining proteinaceous structures embedded in Araldite.
It cannot be used for material fixed in Permanganate, but this should
not affect many people nowadays! Hope you have success with it.
Jim Chalcroft





From: Daniel Possin :      oemlab-at-u.washington.edu
Date: Tue, 10 Jan 1995 17:05:49 -0800 (PST)
Subject: Re: Microscopy lab plans

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Oops -

I just realized that you have a materials microscopy lab. Sorry - I know
some of my ideas and experience is not applicable. Hope some of it
is.

Good luck with the new lab.

Dan

On Tue, 10 Jan 1995, LE Porter wrote:

} We are finalizing plans for a new central microscopy laboratory. In part,
} these plans are modeled after those furnished by R A Harris (University of
} California, Davis) where areas are compartmentalized i.e. cryo microtoming
} in separate room, etc). For general health reasons we have restricted
} solvent usage to one room within and isolated from this larger room. We
} are experiencing some resistance to this concept. Can any of you lend some
} support to this concept? Or tell us why it is not a good idea. Please
} contact me via email, fax or phone with your welcome comments. As ever,
} thanks in advance for your comments.
}
} L E Porter Phone (216) 796-1620
} Head of Microscopy Fax (216) 796-3304
} The Goodyear Tire & Rubber Company EMail LPORTER-at-GOODYEAR.COM
} Dept 415A
} 142 Goodyear Blvd
} Akron, OH 44305
} USA
}
}
}
}




From: tayloe-at-rorc.usbm.gov
Date: Tue, 10 Jan 1995 16:12:06 -0600 (CST)
Subject: Re: Source for Xenon lamps

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On Tue, 10 Jan 1995, ROBERT K. NAUMAN wrote:
} Can anyone provide me with a source for a new 450 Watt Xenon Lamp?
} Thanks!
} Bob Nauman
} Baltimore, MD 21201

May contact Ralph Maxwell in Massachusetts -at- (508) 692-4973. He operates
a company called Remtron that we used to retrofit our old B&L Research I
Metallograph from carbon arc to Xenon. He sells the OSRAM XBO 450W OFR
for about $480 per bulb. If this is not the correct type you need, I'm
sure that he can help ya find the exact type. Also, if memory serves me,
(haa haa!) I think OSRAM may have a facility in New York or somewhere in
the east; altho' the bulbs I have are German-made.

Hope this is of help,
-Rob
,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
| Rob Tayloe | MSM Spelunkers Club /\v/\ |
| Metallographic Lab. | Missouri Speleological Survey /\v/\ |
| Rolla Research Center | Bat Conservation International /\v/\ |
| U.S. Bureau of Mines | Missouri Cave & Karst Conservancy |
| tayloe-at-rorc.usbm.gov | National Speleological Society #32993 /\v/\ |
| (314) 364-3169 x247 | American Cave Conservation Association |
''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''




From: O=PRDSMTP; DDA.TYPE=RFC-822; DDA.VALUE=jerry(a)biochem.dental.upenn.edu
Date: Tue, 10 Jan 1995 11:10:30 -0600
Subject: Bone prep for SEM

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necessary to know what kind of examination is to be done. We have examined
bone and tooth here at our facility at the U. of P. dental research
department and for quick examination of both these mineralized tissues
air-drying has been sufficient. However, we also first examine samples at
10x to 100x in a dissecting microscope while fresh from the source for
micro-fractures to determine if any small cracks we may see in the SEM have
been caused by the drying method or were there at the beginning. To
minimize crystal metamorphosis, the mineral sample is immersed in LN2 and
lyophilized. For a moderately rigorous drying procedure, we do a slow dry
out of Freon 113 and omit critical point drying. This is done by treating
the mineral as any soft tissue--Karnovsky's fixative, followed by an ethanol
drying series and, after two exchanges in 100% ethanol, samples are immersed
in Freon 113. The container with the bone and freon (usually the bottom of a
30 mm petri dish) is covered with parafilm with a few needle holes in it and
allowed to air-dry (a few hours).
An excellent reference is "Methods of Calcified Tissue Preparation"
Elsevier Science Publishing Co., Inc. 52 Vanderbilt Ave., New York, NY
10017. Particularly, Chapter 7 by Alan Boyde.

Good Luck,
Jerry Harrison






From: Daniel Possin :      oemlab-at-u.washington.edu
Date: Tue, 10 Jan 1995 16:51:23 -0800 (PST)
Subject: Re: Microscopy lab plans

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X-Sender: oemlab-at-stein2.u.washington.edu

Hi -

I have no knowledge of the design plans you mention, but I assume that the
solvent usage room has special ventilation and/or hood space. I would
also assume that all the tissue embedding, grid preparation, slide
staining, solution preparation, and tissue fixation (implying the presence
of transmission and dissecting light microscopes) would be done in this
room. If so, the only objection I can think of would involve the
occasional use of small amounts of solvent (ethanol, acetone and xylene)
needed for equipment cleaning, slide coverslipping, and possibly section
mounting in the microtomy area. The necessary amounts of these things
would be very small and it is doubtful that their presence and use in the
microtomy room would constitute a hazard. If a large number of slides
need to be coverslipping at one time, it is, of course, necessary to do it
in a highly ventilated environment - but coverslipping an occasional
individual slide shouldn't require the inconvenience of moving to another
room.

If you plan to separate working with tissues (fixed or otherwise) to
another room, you may generate many inconveniences unless small amounts
of certain chemicals (primarily fixatives, eg. aldehydes) are allowed.

I have worked as a microscopy technologist for approximately 17 years in a
number of settings. I am aware of the safety issues but, also know that
if you place unrealistic restraints on people, they will find a way around
them sooner ar later. The best solutions seem to me to usually involve a
certain amount of compromise.

Dan

On Tue, 10 Jan 1995, LE Porter wrote:

} We are finalizing plans for a new central microscopy laboratory. In part,
} these plans are modeled after those furnished by R A Harris (University of
} California, Davis) where areas are compartmentalized i.e. cryo microtoming
} in separate room, etc). For general health reasons we have restricted
} solvent usage to one room within and isolated from this larger room. We
} are experiencing some resistance to this concept. Can any of you lend some
} support to this concept? Or tell us why it is not a good idea. Please
} contact me via email, fax or phone with your welcome comments. As ever,
} thanks in advance for your comments.
}
} L E Porter Phone (216) 796-1620
} Head of Microscopy Fax (216) 796-3304
} The Goodyear Tire & Rubber Company EMail LPORTER-at-GOODYEAR.COM
} Dept 415A
} 142 Goodyear Blvd
} Akron, OH 44305
} USA
}
}
}
}




From: Richard E. Edelmann :      REDELMAN-at-musom01.mu.wvnet.edu
Date: Wed, 11 Jan 1995 08:52:57 +1100
Subject: Re: Source for Xenon lamps

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WARNING !!!!!

If you are using a Nikon power supply, Nikon recommends that you
DO NOT USE Osram bulbs. For some reason it drastically shortens the
life of the power supply (i.e. shorter than the life of the bulb!).
In our case our Nikon supplier suggested Ushio bulbs.

We specifically have a 75 watts system and the same may not be
true of the 450 watt systems but you may want to find this
information out before you fry your power supply.

(I have nothing against Osram, I regularly use Osram bulbs for
many other applications)



Richard E. Edelmann
Electron Microscopy Facility Supervisor
Marshall University - School of Medicine
Huntington, West Virginia




From: Michael Rock :      merock-at-u.washington.edu
Date: Wed, 11 Jan 1995 08:22:37 -0800 (PST)
Subject: Re: Microscopy lab plans

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keeping the solvents "restricted" to the prep lab is a good idea, however
in reality it is sometimes necessary to us solvents (chloroform, acetone,
ethanol) in thet microtomy or microscope labs.
-Mike




From: John M Hudak :      hudakjm-at-mcmail.cis.mcmaster.ca
Date: Wed, 11 Jan 1995 13:38:54 +0001 (EST)
Subject: re: where to buy freon 113

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You might try: Miller-Stephenson Chemical Co. Inc.
George Washington Highway
Danbury, Connecticut 06810
203-743-4447

____________________________________________________________________
John Hudak hudakjm-at-mcmaster.ca
I.M.R. - Electron Optics
McMaster University, Hamilton, Ontario, Canada






From: Duke, Steve :      DUKE-at-uthscsa.edu
Date: Wed, 11 Jan 1995 13:48:08 -0600
Subject: Tracor Northern NS-880

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Microscopy Users:

Is anyone knowledgeable about Tracor Northern NS-880 EDS x-ray analysis =
system? Specific question: How do you boot from the tape drive when the =
system has had a floppy drive but it is missing?

Any information would be greatly appreciated. Dr. N.K. Smith




From: Ostertag Tom :      ostertag_tom-at-mn15-gw.mavd.honeywell.com
Date: 11 Jan 1995 10:59:06 U
Subject: Freon 113

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Vincent writes:

} Does anyone know where to buy freon 113 in gal. size? Our freon pump
} cooling the Cameca MBX probe needs periodic refill.
}
} Thanks in advance!
}
} Vincent
}
} PS. I really doubt that the heavy molecules like freon 113 can fly
} high to the ozone layer. Most likely they "sink" to the ground level
} and help fighting the ozone created by industrials and autos(?).

Heuer Jim P. writes:

} Try "Fluorinert" Electronic Liquid (FC-77) from 3M Co. (213) 726 6361.
} About $300 for 11 lbs. (3/4 gal.)

I'd suggest a different 3M material, SF-2, which is used as a blanket material
for vapor phase soldering. It might be a better replacement, but that all
depends on what you mean by a Freon pump. Is the material used as a heat
transfer medium or is it evaporated? If the material is used in a closed
system, the FC-77 would be an adequate choice.

Tom Ostertag
ostertag_tom-at-mn15-gw.mavd.honeywell.com





From: tphillips-at-biosci.mbp.missouri.edu (Tom Phillips)
Date: Wed, 11 Jan 1995 12:33:12 -0600
Subject: Sticky secondary antibodies

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We have a user with a nasty problem that we haven't been able to dent.
They are trying to do some immunocytochemistry on HeLa cells growing on
glass coverslips. They fix in either acetone or 2% paraformaldehyde. The
paraformaldehyde fixed monolayers are subsequently permeabilized with
acetone, triton x-100 or saponin (problem is the same regardless). At this
point there is no autofluorescence at any wavelength. When they incubate
the cells in a high quality donkey anti-mouse IgG coupled to Cy5 (Jackson)
there is a very high level on non-specific adhesion. We have tried the
following blocking agents: normal Donkey serum, BSA, gelatin, non-fat dry
milk, or all of the above simultaneously. Pretreating the sections with the
blocking agents has no effect. Using other anti-mouse antibodies coupled
to FITC or rhodamine or a Goat anti-rabbit serum give the same problem.
I should point out we routinely use these antibodies in my own laboratory
following the same protocols and do not get any background binding. We get
the same result when we personally run up the cells so we can't blame it on
the technician. Is there something weird about HeLa cells or something
else I am missing? Do HeLa cells have IgG receptors? Your helpful advice
will be gratefully received.




Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility
3 Tucker Hall
University of Missouri
Columbia, MO 65211
(314)-882-4712 (voice)
(314)-882-0123 (fax)






From: long-at-macro.mse.uiuc.edu
Date: Wed, 11 Jan 1995 18:35:03 -0600
Subject: unsubscribe

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From: long-at-macro.mse.uiuc.edu
Date: Wed, 11 Jan 1995 18:35:49 -0600
Subject: unsubscribe

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unsubscribe




From: Lixin Wang :      lxwang-at-meceng.coe.neu.edu
Date: Wed, 11 Jan 1995 20:17:50 -0500
Subject: SIGN OFF

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Please move my address from this mail list. Thanks.




From: samso-at-tethys.ph.albany.edu
Date: Thu, 12 Jan 1995 09:19:51 EST
Subject: subscribe

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subscribe




From: KINGSLAND, Arlene :      KINGSLAND-at-paprican.ca
Date: Thu, 12 Jan 1995 09:47:03 EST5EDT
Subject: PERSONAL RESEARCH REQUEST

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Message-Id: {MAILQUEUE-101.950112094703.320-at-pap386.paprican.ca}

Fellow Researchers,
If anyone knows of research being done on bowel disorders I have
a sick daughter and her doctors are at the brain-storming stage after
a year of exploring.
This is a personal request, please contact me through my E-Mail
address.
I appreciate any help at this point. Thnaks.


Arlene Kingsland
KINGSLAND-at-paprican.ca




From: :      MELLIOTT-at-prl.pulmonary.ubc.ca
Date: Thu, 12 Jan 1995 10:13:24 +0800PST
Subject: lm-sticky secondary antibodies

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Someone here in our lab also uses HeLa cells, and stains tehm for
coxsackie virus using monocloanl antibodies. He has not had any
problems with sticky secondaries. He did say that HeLa cells can
possess/express Fc receptors. These might be isotype specific
depending on the "strain" of HeLa cells you have.
Otherwise can't think of any other reason, but what concentration of
blocking agents did you try?

Mark Elliott
Pulmonary Research Lab
UBC
Vancouver Canada




From: jester-at-crnjjsgi.swmed.edu (James V. Jester)
Date: Thu, 12 Jan 1995 15:46:55 -0600
Subject: Sticky Secondary Antibody

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Are you using Fab fragments for your secondary antibodies? Generally,
if we have problems it is because we are using the whole antibody. Also
going to affinity purified or the highest specificity we can obtain
usually gives the best results. The addition of serum (ie goat serum
for goat anti-mouse IgG) throughout the incubation is also helpful.

_____________________________________________________________________
| | |
| James V. Jester | Dept Ophthalmology |
| jester-at-crnjjsgi.swmed.edu | UT Southwestern Medical Center |
| TEL (214)648-7215 | 5323 Harry Hines Blvd |
| FAX (214)648-2382 | Dallas, TX 75235-9057 |
|__________________________________|__________________________________|





From: Duke, Steve :      DUKE-at-uthscsa.edu
Date: Thu, 12 Jan 1995 16:30:54 -0600
Subject: Missing Person

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Dear Microscopy Users:

Does anyone know the name and contact information for a person at UC =
Irvine who had three Tracor Northern NS-880 x-ray analysis systems? This =
is as per John Liska, formerly with Tracor Northern--Noran. Information =
is greatly appreciated,,,Dr. N.K.R. Smith




From: jingli-at-vax.ox.ac.uk
Date: Fri, 13 Jan 1995 10:59:40 +0000
Subject: advice

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Sender: jingli-at-vax.ox.ac.uk

Sir: Could you send me information on job openings in microscopy to me.





From: STANSMAN-at-aol.com
Date: Fri, 13 Jan 1995 07:24:26 -0500
Subject: Re: Source for Xenon lamps

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Hi,


In regards to the response concerning xenon bulbs, I'd like toprovide the
following information.

Nikon's 75 watt xenon power supply is usually shipped with Osram 75 watt arc
lamps and that is the recommended supplier, although technically there is no
difference between Osram and Ushio.

Nikon's 100 watt xenon power supply sold outside the USA is shipped with
Ushio 100 watt xenon arc lamps. I believe only ushio manufactures this type
of 100 watt arc lamp.

In Nikon's 100 watt mercury power supply either Osram or Ushio 100 watt
mercury arc lamps of the same specification are usable and performance is the
same.

450 watt xenon bulbs were typically used in Microprojectors used to project
microscope slide images on to a projection screen for use in teaching, CCTV
has now replaced this technique.

Regards,


Stan Schwartz
Manager, Biomedical Instruments
Nikon Inc. Instrument Group
1300 Walt Whitman Rd.
Melville, NY 11747
516-547-8529 Fax 516-547-0306
E-mail NIKONBIO-at-aol.com





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Fri, 13 Jan 1995 13:07:02 -0600 (CST)
Subject: Backing Up Images

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Keith

We use CD ROM for archival image/data storage.
CD Writer's have come down in price quite abit lately.
What I originally paid $5K for is now available for
~$2K and the unit is twice as fast. You may want
to seriously look into these instead of tape.
Blank CD's cost ~$15 and hold ~650Mbytes.

There are several vendors on the market. I am
currently using Pinnacle Micro, but there are
at least 2 other manufacturers out there.

Nestor Zaluzec
ANL




From: ldm-at-apollo.numis.nwu.edu (L. D. Marks)
Date: Fri, 13 Jan 1995 15:35:55 -0600
Subject: Postdoctoral Position

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microscopy-at-aaem.amc.anl.gov, rmglaeser-at-lbl.bitnet

Postdoctoral Position using HREM/Surface Science

A postdoctoral position is available (immediately) exploiting
the unique capabilities of a UHV-HREM (2 Angstroms and 6x10-11
torr) at Northwestern. Connected to this instrument are a number
of analytical (XPS, Auger, SEM) and a GaAs deposition chamber.
The position would involve working on projects involving GaAs
deposition, as well as number of other projects involving small
particles and metal-semiconductor surfaces.

Experience with TEM (HREM preferred) plus surface science
techniques are important.

Send C.V. plus other material to:

L. D. Marks
Department of Materials Science and Engineering
Northwestern University
Evanston, IL 60208
ldm-at-apollo.numis.nwu.edu




From: jacobb-at-ux5.lbl.gov
Date: Sat, 14 Jan 1995 13:37:31 -0800
Subject: Query: indexing micrograph files digitally

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Message-Id: {199501142129.NAA17584-at-ux5.lbl.gov}

We want to set up a computer-based index to our EM lab negative files.
This is a request for your experiences and recommendations of software.
We would like to store and be able to search thumbnail views of each
image as well as assignable fields and keywords. The images themselves
will be kept as negatives, positives, slides and other formats as separate
files. Commercial packages which manage images themselves are reported to
suffer in searching speed, power and reliablity (A.Abernathy, "Managing
your media", MacUser Sept. 1993, Pp. 190-206). Perhaps a database program
that allows thumbnails to be imported would be best.
Your comments invited.

jacob
Jacob Bastacky, MD
1-116
Lawrence Berkeley Laboratory EMail: sjbastacky-at-lbl.gov
University of California Phone: (510) 486-4606
Berkeley, California 94720 Fax: (510) 486-4750





From: Daniel Luchtel :      dluchtel-at-u.washington.edu
Date: Sat, 14 Jan 1995 13:44:06 -0800 (PST)
Subject: Re: Backing Up Images

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X-Sender: dluchtel-at-homer22.u.washington.edu

I for one would greatly appreciate the names of the other two companies
(with telephone numbers if possible). Also, what is your opinion and
experience with the Pinnacle Micro? Any recommendatins?

On Fri, 13 Jan 1995, Nestor J. Zaluzec-Argonne Nat. Lab. wrote:

} Keith
}
} We use CD ROM for archival image/data storage.
} CD Writer's have come down in price quite abit lately.
} What I originally paid $5K for is now available for
} ~$2K and the unit is twice as fast. You may want
} to seriously look into these instead of tape.
} Blank CD's cost ~$15 and hold ~650Mbytes.
}
} There are several vendors on the market. I am
} currently using Pinnacle Micro, but there are
} at least 2 other manufacturers out there.
}
} Nestor Zaluzec
} ANL
}




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sat, 14 Jan 1995 17:41:58 -0600 (CST)
Subject: CD Rom Backups-Multiple Writes

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Multiple Write sessions on a single CD-ROM have been
possible for several years. The problem is that many
inexpensive CD ROM readers donot support Multi-session
formated disks. So if you intend to send/give the data to
someone with a standard format CD reader (i.e. the
run-of-the-mill ~$200-300 variety) you will have to
cut them a new disk. This generally is not a problem
assuming that you already have the CD writer.

Since the whole reason for a visitor to come to a
user facility is to do an experiment and leave with the
data I see no conflict with not "filling" up the disk.
For example if you are in a user facility and a visitor
comes to do some experiments collecting a few hundred
Mbytes of data, then writing a single CD with just that
few 100Mb on it is usually more effective than writing tens of
floppies or several Syquests. The logic to a CD vs a
removable/rewritable media (Syquest,MO, etc..) is that
everyone can generally afford to buy a $200 CD reader
but not everyone can afford to buy a ~$900 MO reader.
This way a "user-facility" can support many type of users
and they do not all have to go out and buy expensive
drives. On the flip side if the user and the facility
both have compatible media then using that media is
reasonable (since I happen to have lots of different
drives and media I can accomodate most users)

For local storage issues, the approach I tend to take
is to have a local disk server (2.5 Gb partitioned into 3-650 MB
segements plus a scratch disk) which is used to
temporairly store data . When a disk partition is full
then it is written to CD (usually 2 copies) and then cleared.
If an individual user wants to archive things this
procedure can be also used. In this case, we
download his/her data overnight to an empty partition
and then write the data and give the user back the
finished CD. Then wipe the partition for the next user.

Generally I tend to avoid multi-session CD's, but have
used them on occassion. You just have to carefully label
the disk. Also when you mount a multi-session CD you get
alot of "virtual" disk icons mounted on your desktop. One
per CD session. So if you get into the habit of writing small
partitions to your CD then you will find lots of icons
cluttering up your desktop. My real desk is bad enough
so I try to avoid the problem propagating to the
screen of my workstation.

Hope this proves useful...

Nestor

---------------------
P.S.
Someone asked for information on phone numbers for
manufacturers of CD writers I pulled this out of MacWorld
Magazine.

Pinnacle Micro RCD-1000 CD writer: 800-553-7070

Most CD writer's are sold direct by the manufacturer
I don't recall seeing very many advertised in the
mail-order catalogs. So you may have to do some hunting.
I'm positive that there are at least 2 other manufacturers
out there of ~$2K writers. I seem to recall Sony
and Chinon, but don't take that as Gospel.

BTW for the record
I have no financial interest in Pinnacle Micro Systems.





From: jingli-at-vax.ox.ac.uk
Date: Mon, 16 Jan 1995 10:50:13 +0000
Subject: Job Openings in Electron Microscopy"

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Sender: jingli-at-vax.ox.ac.uk

Dear Sir: Could you send me job openings in electron microscopy. Thanks.J,Li





From: Katherine.S.Connolly-at-Dartmouth.EDU (Katherine S. Connolly)
Date: 16 Jan 95 09:22:45 EST
Subject: TEM-indexing micrograph files

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Message-id: {10690969-at-dancer.Dartmouth.EDU}

I use Claris Works database to maintain my records and include film numbers for
each record or subject. Any search for an image is slowed by division into
years but I have done retreivals rapidly by using one criterion for search.
Good Luck--
Kate Connolly




From: swatkins-at-pitt.edu (Simon Watkins)
Date: Mon, 16 Jan 1995 09:22:57 -0500
Subject: CD rom archiving

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Like Nestor: We have stopped using tape completely,(anyone want a Jetstore
5000!) having found that we actually needed our backup on occasion. We are
using the Pinnacle RCD1000 currently, it cost -at-$2000 to go in a pc, this
included an Adaptec SCSI board. Generally we generate about 1 Gig/mo of
images from a variety of platforms, Confocal, EM, and LM. However I would
like to make a few comments on the viability of CD's as a principle storage
media

1: They are an absolute boon in a multiuser environment. We give users a CD
and they are gone forever, we do not have to coddle their data for the next
20 years or try and find it on a tape It doesn't matter what platform they
use,(PC/MAC/SGI) the CD works. However this is tempered by two problems.
a: The recordable CD's are not as robust as ones made by commercial
presses and do not like being scratched.
b: The namespace of the platform chosen is important. For example
we use SGI's to analyse all our confocal data however the data may be stored
on a Novell server and accessed through NFS. When the data is archived to
CD, only the DOS name space is recorded, which means that some of the UNIX
names get truncated to 8 letters and then become useless

2:Cd's are geat if you have 650meg to back up. One of the biggest problems
we faced when we started using CD's was how to organize the data. By
project? by date etc. The problem is that unlike a tape or disk where you
can keep adding on forever with a CD you loose a lot of space when the disk
is multisessioned. Effectively the FAT takes up 20MEG and every time you add
something to the disk in a new recording session you have to rewrite the
FAT.... To get round this problem as we ideally we would like to make single
user archives we have a large online resevoir of space (13MEG) and use
650MEG Flop-opticals as an intermediate storage device.

3:You need to buy a 1 Gig drive along with the CD. It is possible to make a
CD with a virtual image which then pulls all the data from the vaious
directories and drives etc. However this is dangerous... A much better
solution is to plant a decent sized hard drive in the machine you will be
archiving and make a single, large ISO compliant file which is written
directly to the disk.

4:When recording you cannot expect the pc to do anything else. This includes
running a screen saver. I estimate that the happy Xmas Screen saver we had
this year cost us about 5 CD's.

5:It is worth thinking about a Juke box player to go with it. We are using
a Pioneed 604X Takes 6 disk cassettes, costs about $1000. Trouble is that
if you want to use it over a net then you must buy multidisk support
software/hardware. Microtest make either diskport (a hardware soln) or
diskserve (a software soln). Either work well.

Generally we are extremely happy with this media, We have Flop-opticals, DAT
and non-DAT backup devices. CD's are really the only way to go though they
are cheap ($15/650meg) relatively fast and perhaps most importantly now that
almost all PC's have CD players in them, distributable!


----------------------------------------------------
Simon Watkins Ph.D
Director Structural Biology Imaging Center
Scaife 840
University of Pittsburgh
Pittsburgh PA 15261

Tel:412:648-3051
Fax:412:648-1441
----------------------------------------------------





From: swatkins-at-pitt.edu (Simon Watkins)
Date: Mon, 16 Jan 1995 09:26:58 -0500
Subject: Re: Query: indexing micrograph files digitally

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} We want to set up a computer-based index to our EM lab negative files.
} This is a request for your experiences and recommendations of software.
} We would like to store and be able to search thumbnail views of each
} image as well as assignable fields and keywords. The images themselves
} will be kept as negatives, positives, slides and other formats as separate
} files. Commercial packages which manage images themselves are reported to
} suffer in searching speed, power and reliablity (A.Abernathy, "Managing
} your media", MacUser Sept. 1993, Pp. 190-206). Perhaps a database program
} that allows thumbnails to be imported would be best.
} Your comments invited.

The new version of HiJack Pro solves many of these problems in a cost
effective ($90) fashion. It works differently to the earlier version and
keeps a central database of images which can be accessed very rapidly. It
works accross servers, allows multiple indexes, does thumbnails, and updates
itself automatically, comes with a montaging and editing program. We love it


----------------------------------------------------
Simon Watkins Ph.D
Director Structural Biology Imaging Center
Scaife 840
University of Pittsburgh
Pittsburgh PA 15261

Tel:412:648-3051
Fax:412:648-1441
----------------------------------------------------





From: Laurie Frederick :      frederick_laurie-at-vanlab.paprican.ca
Date: Mon, 16 Jan 1995 09:47:37 PDT
Subject: CD-ROM referances

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Hi,
I am currently looking into switching to digital image archiving and
have found two referances which may be of assistance to others in
the same position:

- There is a review of cd-rom drives in "PC Computing", Nov., 1994.
This supplies name and phone number for several manufacturers as
well.
- Kodak offers a CD-ROM drive guide which lists CD-ROM drives,
controller cards and device driver software combinations which are
compatabile with Kodak Photo CD. Not all combinations are equal. I
have publication DCI-249 dated 8/94 which lists combinations for PC
and Mac. I don't know what is available for Unix.

Hope this is helpful.
Laurie

______________________________________________________________________
Laurie Frederick, A.SC.T. PAPRICAN
Corrosion Control Group 3800 Wesbrook Mall
The Pulp and Paper Research Vancouver, B.C.
Institute of Canada Canada V6S 2L9

Email: frederick_laurie-at-vanlab.paprican.ca
Tel: 604-222-3200 Fax: 604-222-3207




From: JOY-at-utkvx.utk.edu (DAVID JOY)
Date: Mon, 16 Jan 1995 14:00:56 -0500 (EST)
Subject: 14th International Congress IXCOM

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14th International Congress on X-ray Optics and Microanalysis
August 29 - Spetember 2nd 1995
GuangZhou, CHina

This congress will be held, 39 years after the first UK congress, in GuangZhou,
a city only two hours by train from Hong Kong. The program will include the
following topics: X-ray Optics, Sources and Microscopy; X-ray Photoelectron
Spectroscopy; Analytical Electron Microscopy (AEM); Scanning Electron
Microscopy; Electron Probe Microanalysis; Auger Electron Microscopy; Ion
Probe and Secondary Ion Mass Spectroscopy; Laser Probe; Scanning Probe
Microscopies
(STM, AFM); and Confocal Microscopy.

Contributed papers prepared in 2 or 4 camera ready pages with text and figures
inside a frame of 144mmx210mm will be published in Proceedings available to
each participant at the Congress. A comprehensive Trade Exhibition will also
be scheduled.

The deadline for contributed papers is April 30th, 1995, and the early
registration fee, thru June 30th, is US$350. The accomodation rate (including
three meals) in a three star hotel is US $50-85 per day. The organizing
committee will provide arriving participants with transportation from
GuangZhou International Airport and the railroad station on Aug.29th.

Second circulars, registration forms and contributed paper format are now
available from

Secretariat of 14th IXCOM, Foreign Affairs Division, Guang Zhou Branch,
CAS, 100 Xioanlie Road, C.GuangZhou 510070, CHina.
Tel + 8620 777 5213 Fax +8620 777 5791






From: Parry, Althea :      ParryA-at-agresearch.cri.nz
Date: Tue, 17 Jan 1995 09:53 +1200 (NZST)
Subject: unsubscribe

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From: Doug Arrell :      ARRELL-at-jrc.nl
Date: Tue, 17 Jan 1995 08:40:02 GMT+0200
Subject: Re: CD ROMS

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Message-Id: {MAILQUEUE-101.950117084002.352-at-FS-IAM-1.JRC.NL}

On the subject of CD ROMS writers, I looked through a British
computer magazine and found advertisments for a few manufacturers,
of the ones likely to be available in the US try Philips (UK price
~$3000) or Yamaha who manufacture a quad-speed writer (~$5000).
However prices in the UK tend to be much higher than in the US, so
they could be considerably less over there. I hope this is of use to
somebody!

Doug Arrell
+------------------------------------+
| Dr Douglas Arrell |
| Mechanical Performance and Joining |
| Institute for Advanced Materials |
| 1755 ZG Petten |
| Netherlands |
| {ARRELL-at-JRC.NL} |
| Tel. (+31) 2246 5287 |
| Fax (+31) 2246 1917 |
+------------------------------------+




From: fskarl-at-goodyear.com (Frank Karl)
Date: Tue, 17 Jan 1995 10:57:45 -0500
Subject: Optical properties of FeCl3 (PLM)

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I am in need of optical crystallographic properties for FeCl3 and
FeCl3 - nH20. Winchell's Optical Properties of Artificial Minerals has the
+2 valence state of iron, but not the +3 state.


Any assistance would be greatly appreciated!

Thanks............


Frank Karl fskarl-at-goodyear.com
Goodyear Tire & Rubber Co. Voice 216.796.7818
Analytical Services - Dept 415B Fax 216.796.3304
142 Goodyear Blvd
Akron, OH 44305
U.S.A.









From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Tue, 17 Jan 1995 11:43:38 -0600 (CST)
Subject: Post Doc Opening

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From: mrdunlap-at-ucdavis.edu (Michael Dunlap)
Date: Tue, 17 Jan 1995 11:57:05 -0800
Subject: Re: CD-ROM referances

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Hello -

The June 94' issue of MacUser has a good article on CD-ROM writers listing
a number of different companys and MacUser's ideas on the pros and cons of
each.
Included is MacUser's commemnts on different software that is available for
the writers. I beileve PC Week had a simular article.

Mike

---------------------------------------------------------------------------
| Michael Dunlap | lab (916) 752-0284 |
| Facility For Advanced Instrumentation | fax (510) 422-2282 |
| University of California | mrdunlap-at-ucdavis.edu |
| Davis CA, 95616 | |
===========================================================================






From: John Mansfield :      John_Mansfield-at-mse.engin.umich.edu
Date: 16 Jan 1995 16:51:09 -0400
Subject: Computer: New files on...

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Message-ID: {n1421759759.17687-at-mse.engin.umich.edu}

John Mansfield
North Campus Electron Microbeam Analysis Laboratory
413 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (313)936-3352 FAX (313)936-3352
jfmjfm-at-engin.umich.edu or John.F.Mansfield-at-umich.edu Time: 4:39
PM

Date:1/16/95
URL: http://www.engin.umich.edu/~jfmjfm/jfmjfm.html NC EMAL

Three software packages have been added/modified in the Microbeam Analysis
Society Software Library (MASSL) in recent months.
The MASSL resides on freebie.engin.umich.edu
username: anonymous
password: your email address in the form user-at-domain.
Directory: /pub/MSA+MAS/MASSL
New files in:
1. /pub/MSA+MAS/MASSL/xphi
A Full abstract was not included by the author of this program. Here is a
summ
ary of the information that he supplied in printed form.

Xphi is a PhiRhoZ correction program. For a description see:
"An Accurate Computer Correction Program for Quantitative Electron Probe
Microa
nalysis", Claude Merlet, Mikrochim. Acta 114/115 363-376 (1994).
Install the program by exploding the auto-unzip file xphiz.exe into a
directory
on your hard drive (say, c:\xphi). Install the fonts, FIX8X14.FON and
FIX8X16.F
ON that come with the program in the Windows sytem directory (e.g. in
c:\windows
\system). The program is called xphi.exe, add the program to your program
manag
er listing so that you may double click it to run it from the windows shell.

2. /pub/MSA+MAS/MASSL/shrli
A program called Simply Shrli, designed to run on the PC. This from the
readme file.
SIMPLY : WHAT IS IT FOR ?
*************************
SIMPLY is a set of programs dedicated to Transmission Electron Microscopy in

Materials Sciences. It allows you to BUILD structures, DRAW and HANDLE cells,
CALCULATE, DISPLAY and HELP to INDEX diffraction patterns, CALCULATE and DIS-

PLAY High Resolution IMAGES.
SIMPLY runs "SHRLI", (c) M.A.O'KEEFE, (1980). .../...

SIMPLY first appeared as:
"SIMPLY: a package for the SIMulation and disPLaY of HREM images on PCs",
T. EPICIER, M.A.O'KEEFE, communication at 33rd Ann. Meeting of the French
Electron Microsc. Soc. (SFME), Villeurbanne-Lyon - F., june-july 1993.
It has been demonstrated during the "Computers Open lab", at the 13th Intern.
Congress on Electron Microscopy (ICEM 13), Paris - F., 17-22 July 1994.

3. /pub/MSA+MAS/MASSL/KAKER
Two abstracts here in the requested format (MANY THANKS Henrik!!!).
Title : Edax
Keywords : XEDS,SEM
Computer : IBM PC or compatible
Operating System : MS-DOS
Programming Language : Turbo Basic,GWBasic
Hardware Requirements : EDS Edax 9100,RS232C Card
Author(s) : Henrik Kaker
Correspondence Address : SEM/EDS Laboratory,.Metal d.o.o.,Koroska c.14
: 62390 Ravne,Slovenia,E-mail:kaker&ctklj.ctk.si
Abstract:

Program for connection energy dispersive spectromter Edax 9100 with IBM
PC or compatible computer via line printer port of the Edax 9100.Program
allows transfering intensity and spectral data to PC and processing this
data with standardless programs for bulk and thin film samples.

Documentation: EDAX.DOC

Source Code: Yes

and

Title : EPMA Database
Keywords : EDS, WDS, SEM, EPMA
Computer : None
Operating System : None
Programming Language : None
Hardware Requirements : None
Author(s) : Henrik Kaker
Correspondence Address : SEM/EDS Laboratory,.Metal d.o.o.,Koroska c.14
: 62390 Ravne,Slovenia,E-mail:kaker&ctklj.ctk.si
Abstract:

Database of 681, 826 and 40 published entries for performance testing
of EPMA programs. Database EPMA40.DAT present collection of published data
from different manufactures of EDS and WDS hardware and software and is
suitable for testing standardless (no-standard) analysis programs.

Documentation: EPMA.DOC

Source Code: No

Two other points of information.
A.
Some of the archived files have been found to be corrupted and even our
backups are no good. If anyone has good copies of the following files,
please send me copies.
They are under pub/MSA+MAS/MASSL
1) ~/ECPORI/ECPORI.SIT
2) ~/FINDCSL/FINDCSL.SIT
B. The MASSL and the Electron Microscopy and Microanalysis Public Domain
Libraries will be merged into one library, however this will only occur when
Nestor Zaluzec get chance to sit down and organise the files. So, please be
patient.
Many thanks.


John Mansfield.

Manyt thanks.










From: Willem.Jacob :      jacob-at-sch2.uia.ac.be
Date: Wed, 18 Jan 1995 10:18:05 +0100 (MET)
Subject: contrast problems in EM

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Dear Em specialists,
since a few months we are dealing with a stupid problem: every time we
prepare samples for classical em investigation we sometimes have
contrast and sometimes not. The procedure we uses is:
3% glutaraldehyde fixation (from 1 hour to overnight), wash in 0.1 M
cacodylate (approx 30 min)
buffer, osmiumtetroxide fixation (1 or 2% in caco for approx 1h), again a
wash in caco (30 min), dehydration in a ethanol series (70, 90, 2x100
every step 30 min) then a 2X10min incubation in propylene oxide, an
overnight impregnation in 1:1 epon (lx): propylene oxide and finally an
embedding in lx at 60 degrees for 60 hours. After sectionning, the
sections are contrasted for 2 to 3 min with 5 % uranylacetate
in water and finally 1 min lead citrate. Every time we use exactly the
same procedure but the contrast varies
from perfect to absent. We have already tried to change the fixatives and
contrasting solution , prepare fresh, change lot, change companie but
nothing helped. Also we changed the incubation times but now ower
inspiration is gone so ... who can help us solving this irritating problem?
Thanks for your help.
Annette bakker






From: SveEn-at-pai.liu.se (Sverker =?iso-8859-1?Q?Enestr=F6m?= )
Date: Wed, 18 Jan 1995 13:48:43 +0100
Subject: EM: contrast problems

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Annette wrote:
} since a few months we are dealing with a stupid problem: every time we
} prepare samples for classical em investigation we sometimes have
} contrast and sometimes not. The procedure we uses is:
} 3% glutaraldehyde fixation (from 1 hour to overnight), wash in 0.1 M
} cacodylate (approx 30 min)
} buffer, osmiumtetroxide fixation (1 or 2% in caco for approx 1h), again a
} wash in caco (30 min), dehydration in a ethanol series (70, 90, 2x100
} every step 30 min) then a 2X10min incubation in propylene oxide, an
} overnight impregnation in 1:1 epon (lx): propylene oxide and finally an
} embedding in lx at 60 degrees for 60 hours. After sectionning, the
} sections are contrasted for 2 to 3 min with 5 % uranylacetate
} in water and finally 1 min lead citrate. Every time we use exactly the
} same procedure but the contrast varies
} from perfect to absent. We have already tried to change the fixatives and
} contrasting solution , prepare fresh, change lot, change companie but
} nothing helped. Also we changed the incubation times but now ower
} inspiration is gone so ... who can help us solving this irritating problem?
} Thanks for your help.
} Annette bakker


Hello Annette!
I think we all have had more or less problems getting optimal contrast in
our specimens
but there are also the ugly precipitates which can terrify us.
We poststain with UAc (2.5% ethanol solution) for 10 min or with 5% water
solution of UAc
for 20-30 min, followed by staining with fresh Pb-citrate for 2 min. I
think your staining
periods are too short.
The staining results are also depending on what tissue you work with. UAc
reacts prin-
cipally with nucleic acids and proteins. Pb-citrate increases the general
contrast of
membranes and also stains nucleic acid, glycogen and proteins due to the
presence of
carboxyl and sulfhydryl groups.
Good luck and feel free to contact me for further discussions.
Sverker Enestr|m, M.D., Ph.D.
Department of Pathology
Link|ping university, Sweden

=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=
=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=
=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD
Sverker Enestr|m
Tel: +46 13 22 15 20
=46ax: +46 13 13 22 57
=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=
=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=
=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD=hD






From: Jay Jerome :      jjerome-at-isnet.is.wfu.edu
Date: Wed, 18 Jan 1995 08:56:49 -0500 (EST)
Subject: RE: Image enhancement

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Kem Rogers asked about image enhancement for cytochemistry

Here is my two cents worth,
As an ardent image processor enhancer, I have no apriori bias against
using image enhancement. However, in cytochemistry if you are comparing
two things, I think both images should be taken under the same conditions
and processed equivalently. Kem mentions processing to remove background,
and under the circumstances he outlines this seems reasonable. However,
I think background substraction should be done with great care.
One mans background could be
another mans signal. If you have measured background under conditions where
no signal is possible and then subtract it uniformly from all images that
seems reasonable. However, I have had people ask me to remove this defect
or that "small area of background staining". I think that begins to
constitute scientific fraud. Thats my humble opinion.


Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-isnet.is.wfu.edu *
**************************************************************





From: Michael Rock :      merock-at-u.washington.edu
Date: Wed, 18 Jan 1995 10:53:46 -0800 (PST)
Subject: Re: contrast problems in EM

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concerning your staining problem:
1) try doing an "en bloc" stain with 2-4% UA, after OsO4 and your buffer
wash, and just before your dehydration step.
simply immerse your tissue in UA for 30-90 min (keep at 4 degrees C)
then proceed with dehydration
2) when staining your sections with UA and Pb citrate, staining times should
be 10-15 min. in each stain




From: DIRK DOMASCHKO :      DOMASCHK-at-musom01.mu.wvnet.edu
Date: Wed, 18 Jan 1995 13:46:12 +1100
Subject: Re: contrast problem

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Message-Id: {9501182059.AA11409-at-riker.ml.wpafb.af.mil}

Annette,
I agree with Dr. Sverker. The staining times are too short. I
stain for 25 min in uranyl acetate and 15 min in Pb citrate. These
times may seem excessive but we have never had a problem with
contrast. Additiionally, more than one rinse is suggested by my
sources (at least 3/15 min each) in rinse buffer to remove excess
glutaraldehyde. This should eliminate any interaction between
unreacted glutaraldehyde and OsO4 which will cause peppering and
general visual noise in your sections.

Hope this is helpful!

Dirk W. Domaschko
Marshall University
Huntington, WV
Domaschk-at-musom01.mu.wvnet.edu




From: Jay Jerome :      jjerome-at-isnet.is.wfu.edu
Date: Wed, 18 Jan 1995 17:18:24 -0500 (EST)
Subject: Re: TEM: Grid Glue Recipe?

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In addition to the sticky from cellophane tape, thick sections which are
notoriously difficult to keep in place can be stuck to grids using
unpolymerized epoxy resin. Dip one side of the grid to the top of a drop
of epoxy, use compressed air to blow away excess resin from the open
spaces, adhere the section and cure the resin in the oven.


Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-isnet.is.wfu.edu *
**************************************************************





From: oshel-at-ux1.cso.uiuc.edu (Phil Oshel)
Date: Wed, 18 Jan 1995 13:05:23 -0600
Subject: Re: contrast problems in EM

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I'm not allowed to give the name of my reference, but "word has it"
that the pH of the lead citrate is very important: if it is } 12, there will
be little staining, and it may in fact bleach the sections (and lots of ugly
contamination if much {12). Something to be checked each time the lead
citrate is made.
Also, small, unobvious changes in pipette bore, or how the pipette
is held can cause significant changes in drop size, therefore in amount or
concentration of solution delivered.
Phil Oshel
U Illlinois Center for Electron Microscopy
oshel-at-ux1.cso.uiuc.edu
At 10:18 AM 1/18/95 +0100, Willem.Jacob wrote:
} Dear Em specialists,
} since a few months we are dealing with a stupid problem: every time we
} prepare samples for classical em investigation we sometimes have
} contrast and sometimes not. The procedure we uses is:
} 3% glutaraldehyde fixation (from 1 hour to overnight), wash in 0.1 M
} cacodylate (approx 30 min)
} buffer, osmiumtetroxide fixation (1 or 2% in caco for approx 1h), again a
} wash in caco (30 min), dehydration in a ethanol series (70, 90, 2x100
} every step 30 min) then a 2X10min incubation in propylene oxide, an
} overnight impregnation in 1:1 epon (lx): propylene oxide and finally an
} embedding in lx at 60 degrees for 60 hours. After sectionning, the
} sections are contrasted for 2 to 3 min with 5 % uranylacetate
} in water and finally 1 min lead citrate. Every time we use exactly the
} same procedure but the contrast varies
} from perfect to absent. We have already tried to change the fixatives and
} contrasting solution , prepare fresh, change lot, change companie but
} nothing helped. Also we changed the incubation times but now ower
} inspiration is gone so ... who can help us solving this irritating problem?
} Thanks for your help.
} Annette bakker
}
}
}
}
Phil Oshel
oshel-at-ux1.cso.uiuc.edu
Center for Electron Microscopy
Univ. of Illinois (Urbana)
(217) 244-3135





From: Jan Coetzee EM Univ Pretoria :      JANC-at-ccnet.up.ac.za
Date: Thu, 19 Jan 1995 08:24:56 GMT+2
Subject: EM Lab scheduling

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We operate 7 electron microscopes (4 scanners, 2 TEM's, 1 microprobe),
optical photomicroscope, 2 ultramicrotomes, materials science specimen prep
equipment, high vacuum coater, ion beam coater, darkroom, etc - pretty much
the generic general EM lab.
At present we use a paper-based (dog-eared diary) booking system for
scheduling users on the equipment.

I know that there are many labs that use electronic booking systems.
Is this worthwhile, or does it cause more problems than it solves?
Nearly all our users have access to ethernetted PC's.
Information on pros and cons, problems, solutions, which software etc
would be most useful. Our LAN superviser is especially interested in being
pointed in the right direction for sources of software that will do the job.


Jan Coetzee
Unit for Electron Microscopy Tel:+27-12-420-2075
University of Pretoria Fax:+27-12-342-1738
Pretoria Internet:janc-at-ccnet.up.ac.za
0002 South Africa




From: ldm-at-apollo.numis.nwu.edu (L. D. Marks)
Date: Thu, 19 Jan 1995 07:37:17 -0600
Subject: Re: EM Lab scheduling

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I implimented this type of approach back around 1990, and have found
it to be very useful. (My source code is very simple since it is designed
for VT100 emulation across the ethernet, so I would not recommend it.) Apart
from simple security issues such as making sure that authorized (safe) people
only use the microscopes, the biggest advantage is speed. Noone has to spend
hours collating the pages, and you do not have to worry about honesty -- how
many people write down all the hours that they use?
The major issue is not the software (trivial to write) but arranging
electronic locks for the hardware such that they can only be used with an
appropriate password (different for each user). We got an electronics technician
to build a simple card for our TEM to control the beam. I would be interested
in hearing of any more general solutions.




From: John Mansfield :      John_Mansfield-at-mse.engin.umich.edu
Date: 19 Jan 1995 10:38:58 -0400
Subject: Re: EM Lab Scheduling

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Reply... RE} EM Lab Scheduling
Reply
from: John Mansfield
North Campus Electron Microbeam Analysis Laboratory
University of Michigan
2455 Hayward
Ann Arbor MI 48109-2143
Phone: (313)936-3352 FAX: (313)936-3352
jfmjfm-at-umich.edu or John_Mansfield-at-mse.engin.umich.edu
URL: http://www.engin.umich.edu/~jfmjfm/jfmjfm.html

Here at Michigan our lab serves users from many departments (nuclear,
materials, chemical , electrical, civil and mechanical engineering, physics,
chemistry, geology, pediatric cardiology and pharmacy to mention a few) and
they are spread out all over the city of Ann Arbor (about a five mile
radius).
Since there are University computer sites all over campus and many people
have Macintosh computers or can at least get access to them and there is a
campus-wide Appletalk network, we us a simple scheduling system based on a
collection of Hypercard stacks.
Each microscope, or other instrument that is schedulable, has a stack
associated with it. These stack are stored on a server in our lab and are
accessible from anywhere on campus. Each stack is a basic calendar with the
days of the week listed in four hour block for the day time (~8-5) and 8 hour
blocks in the evening and at night. Clicking on any one block of time allows
the user to book that entire block or part of it. The user needs a user name
and password and trained users are issued these ids that are stored within
the stack. users can book up to 1 week in advance normally but I have the
option of overriding that. When the stack reaches a certain size it is
archived and the current data is copied to a new stack. It is not a really
secure system but it works well for us.
If anyone wants a copy of any of the stacks to modify for their own purposes,
I will make them available on freebie.engin.umich.edu. The proviso is that
any improvements should be made available to everyone, us especially!.
OK?
Just my 2 cents worth.
Jfm.





From: randy nessler :      rnessler-at-emiris.iaf.uiowa.edu
Date: Thu, 19 Jan 1995 16:31:03 -0600 (CST)
Subject: Light element EDS

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Message-Id: {9501192124.AA15279-at-riker.ml.wpafb.af.mil}


I am interested in the discussion of light element analysis using
a Kevex Quantum detector. From my discussions with Kevex, it seems that
the software available has a hard time compensating for matrix effects
when light elements are mixed with high atomic number elements. I was
wondering how other people deal with this. Our system runs on RT11, so
people who have TSX systems will have to bear with me. It appears that
the answer is standards, standards, and more standards. Let's hear it...
Randy Nessler







From: Alan Hall :      HALL-at-agric.up.ac.za
Date: Fri, 20 Jan 1995 10:34:11 GMT+2
Subject: Critical Point Drying

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A couple of questions on CPD:
1. How important is it to use CO2 from a cylinder furnished with an ejector
tube?

2.Can one use a conventional cylinder turned upside-down?

3.Am I right in assuming that cooling the CPD-chamber down to { 0 C, should
condensate the CO2 to a liquid?

4.What grade (purity) should one use? I have always used "Medical Grade",
obviously at a higher cost than "Technical Grade"

Discussion would be appreciated.Alan Hall
Unit for Electron Microscopy
University of Pretoria, Pretoria Tel +27-12-420-3297
South Africa Fax +27-12-420-3266




From: CAROLYN J. EMERSON, DEPT. OF BIOLOGY, MEMORIAL UNIVERSITY
Date: Fri, 20 Jan 1995 09:50:12 -0230
Subject: SEM filters

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Message-ID: {950119110849E68.AERO-at-USCN.USCN.UGA.EDU} (UMass-Mailer 4.04)
{cemerson-at-kean.ucs.mun.ca}
Reply-To: cemerson-at-kean.ucs.mun.ca
To: MICROSCOPY-at-AAEM.AMC.ANL.GOV
Message-ID: {0098ABD3.AF64FCE1.1-at-leif.ucs.mun.ca}

Could anyone please direct me to sources of Anopore disc filters or
Flotronics filters for use in support of particulate samples for
SEM viewing. A Canadian or US supplier would be helpful. Thanks
Carolyn J. Emerson
Biology Dept.
Memorial University
St. John's Newfoundland Canada
cemerson-at-kean.ucs.mun.ca




From: Jeffrey.Shield-at-mse.utah.edu (Jeffrey E. Shield)
Date: Fri, 20 Jan 1995 08:55:55 -0700
Subject: Reflection electron microscopy wanted

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To all:

I have someone interested in doing some reflection electron microscopy. Not
being the surface type, I am unable to assist. If anyone out there is
remotely interest in doing some REM please contact me directly for more
details.

Thanks for your time.

Jeff
--------------------------------------------------------- U U
| | U U
| Jeff Shield | U U
| Department of Materials Science and Engineering | U U U U
| University of Utah | U U U U
| Salt Lake City, UT 84112 | U U U U
| 801/581-3179 | UUUUU U
| Fax: 801/581-4816 | U U
| | U U
--------------------------------------------------------- UUUUU

Of making many books there is no end, and much study wearies the body.
-Eccl 12:12





From: jacobb-at-ux5.lbl.gov
Date: Fri, 20 Jan 1995 10:08:09 -0800
Subject: Re: SEM parts

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Message-Id: {199501201800.KAA15728-at-ux5.lbl.gov}

AMRAY has been very good about supplying parts for our 1000A (still our
workhorse 4-6 full days per week). Their telephone number is 800-225-1462.
We've also had to replace some of these switches after some years.

} We are trying to repair the push-push type switches in the control
} panel of our AMR 1000 SEM. The failure is mechanical rather than electronic.
} The switches have "Master Specialties Co." writen on them and are in the
} 12-4 series. Any line on where we can beg or buy at least two such switches
} would be greatly appreciated.
} Thanks in advance.
} "For want of a nail...."
} Tom Hanley
} 706 568-2075
} thanly-at-uscn.bitnet

Jacob
Jacob Bastacky, MD
1-116
Lawrence Berkeley Laboratory EMail: sjbastacky-at-lbl.gov
University of California Phone: (510) 486-4606
Berkeley, California 94720 Fax: (510) 486-4750





From: John Mansfield :      John_Mansfield-at-mse.engin.umich.edu
Date: 20 Jan 1995 13:45:59 -0400
Subject: Re: CD ROM image archive

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Reply... RE} CD ROM image archive
Reply
from: John Mansfield
North Campus Electron Microbeam Analysis Laboratory
University of Michigan
2455 Hayward
Ann Arbor MI 48109-2143
Phone: (313)936-3352 FAX: (313)936-3352
jfmjfm-at-umich.edu or John_Mansfield-at-mse.engin.umich.edu
URL: http://www.engin.umich.edu/~jfmjfm/jfmjfm.html

While we are on the subject, has anyone seen the Pinnacle Micro drive for
$1995 that is a double speed reader and also a CD-R drive?
Sounds really nice!





From: SveEn-at-pai.liu.se (Sverker Enestr|m)
Date: Sat, 21 Jan 1995 12:24:13 +0100
Subject: REM

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Good morning,

You can read about reflection microscopy (which visualizes zones of
cellular attachment
to glass in epi-illuminated specimens by surface reflection interference)
in chapter 5 in
ELECTRONIC LIGHT MICROSCOPY, edited by David Shotton, Wiley-Liss Inc.,
1993. Modern REM
makes use of video-enhanced contrast technic, well described by David Shotton.

Sverker

==================================================================
Sverker Enestrom
Tel: +46 13 22 15 20
Fax: +46 13 13 22 57
==================================================================






From: nederlof-at-genmic.biochem.mpg.de (Petra Nederlof)
Date: Sun, 22 Jan 1995 13:26:40 +0100
Subject: unsubscribe

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subscribe nederlof-at-genmic.biochem.mpg.de

***************************************
Petra M Nederlof, Ph.D.
Max-Planck-Institute for Biochemistry
Dept. Structural Biology
Am Klopferspitz 18 a
D-82152 Martinsried (M=FCnchen)
GERMANY

nederlof-at-genmic.biochem.mpg.de
voice: +49 (0)89 8578 2624
fax: +49 (0)89 8578 2641






From: sje-at-po.CWRU.Edu (Steven J. Eppell)
Date: Mon, 23 Jan 1995 11:48:25 -0500
Subject: Networking a dye sublimation printer

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I have a Tektronix Phaser II SDX printer that I want to put
on my campus network. I bought the Ethernet adaptor card but
my network administrator is telling me it's not possible to
put the printer on our network. We support Novell and TCP/IP.
Has anyone put this printer on a network using either of
these protocols? If so, can you give me any words of
wisdom that might help me get the printer on the net?

Steven J. Eppell
Center for Cardiovascular Biomaterials
Case Western Reserve University

sje-at-po.cwru.edu




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Mon, 23 Jan 1995 10:55:32 -0600 (CST)
Subject: Student Scholarships to MAS-95 & 96

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MICROBEAM ANALYSIS SOCIETY STUDENT SCHOLARSHIPS TO MAS-1995 (Breckenridge, CO)
and IUMAS-1 (Sydney Australia)

All students (and faculty) involved in microanalysis-related research, should
note a remarkable opportunity for travel to two forthcoming microanalysis
conferences. The Microbeam Analysis society is offering student scholarships to
the 29th Annual MAS meeting in Breckenridge CO (August 6-11, 1995). Student
papers must be submitted, in standard MAS-MSA format to Dr. E. Etz, NIST,
Division 837, Bldg. 222, room Q-113, Gaithersburg, MD 20899 by March 15, 1995.
The best submitted papers will be awarded funds towards attending the annual
meeting in Breckenridge. Any more information about the program can be obtained
from Dr. Etz at etz-at-gapnet.nist.gov

What makes this scholarship offer extraordinary is that the best three papers
given by student scholarship winners at the Breckenridge meeting will be
awarded a minimum of $500 towards the cost of attending the 1st
International Union of
Microbeam Analysis Societies Conference in Sydney, Australia, February 5-9,
1996. These scholarships are only open to student members of MAS, and student
application forms for MAS are available in past issues of Microbeam Analysis,
the MAS journal. Student membership is a great bargain at $2.50, and doesn't
require that the advisor be a MAS member - although if you aren't, I ask that
you consider joining.

I will mail you more information with appropriate details about the meetings
and the paper format etc., but if you have any immediate questions, please don't
hesitate to contact me by email (dbw1-at-lehigh.edu).

Dave Williams






From: Michael Rock :      merock-at-u.washington.edu
Date: Mon, 23 Jan 1995 09:20:32 -0800 (PST)
Subject: Re: Networking a dye sublimation printer

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we connercted our printer up for multiple users via the ethernet connection
-first check to see that the installed tag is next to the ethernet port
(if not you need a bourd installed)
-connect to the ethernet port
-load the phaser utilities, the network utilities, and the drivers
-there are a few entries (words/code #'s/and commands) needed to load it
all and get it going
-if all else fails call Tektronix

good luck
Mike

On Mon, 23 Jan 1995, Steven J. Eppell wrote:

} I have a Tektronix Phaser II SDX printer that I want to put
} on my campus network. I bought the Ethernet adaptor card but
} my network administrator is telling me it's not possible to
} put the printer on our network. We support Novell and TCP/IP.
} Has anyone put this printer on a network using either of
} these protocols? If so, can you give me any words of
} wisdom that might help me get the printer on the net?
}
} Steven J. Eppell
} Center for Cardiovascular Biomaterials
} Case Western Reserve University
}
} sje-at-po.cwru.edu
}




From: noran!tanagra!kburton-at-uunet.uu.net (Kevin Burton)
Date: Mon, 23 Jan 1995 11:54:16 +0600
Subject: Re: Networking a dye sublimation printer

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} } } } } "Steven" == Steven J Eppell {uunet!po.CWRU.Edu!sje} writes:

Steven} I have a Tektronix Phaser II SDX printer that I want to
Steven} put on my campus network. I bought the Ethernet adaptor
Steven} card but my network administrator is telling me it's not
Steven} possible to put the printer on our network. We support
Steven} Novell and TCP/IP. Has anyone put this printer on a
Steven} network using either of these protocols? If so, can you
Steven} give me any words of wisdom that might help me get the
Steven} printer on the net?

Steven} Steven J. Eppell Center for Cardiovascular Biomaterials
Steven} Case Western Reserve University

Steven} sje-at-po.cwru.edu

If you are trying to print from a Unix workstation you can use the
standard BSD print spooling mechanism to print to a network node (the
IP address assigned to the Ethernet adaptor. If this is a Novell
network and you need to print from a Sun Unix workstation you can use
a package from Puzzle Systems called SoftNet Utilities that allows
bidirectional printing.

--
Kevin Burton
Noran Instruments voice: (608) 831-6511 x317
2551 West Beltline Highway, Room 532 FAX: (608) 836-7224
Middleton, WI 53562 email: kburton-at-noran.com

Opinions expressed herein apparently spontaneously organized themselves.






From: sje-at-po.CWRU.Edu (Steven J. Eppell)
Date: Mon, 23 Jan 1995 11:31:21 -0600 (CST)
Subject: Networking a dye sublimation printer

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All subscribers Please remember to
change your default address of "microscopy" to

Microscopy-at-aaem.amc.anl.gov

ANLEMC is dead, and mail is aliased to the new site.

Unfortunately the alias does not always work so some
messages to microscopy do not always come through.

---------------------------
As for your Tek IISDX....

You should likely talk to Tektronics people on
your network problem. I have a IISDX which is
on an Appletalk/Ethernet connection and works fine.
There are special drivers which you must load
for using the printer on Mac, PC, or Sun workstations.
You should have gotten these with your hardware.
You could also run a direct line between the printer
and your workstation.

On the Mac side you simply, plug-in and run. Virtually
no setup time is required, except to copy the drivers
from the disks to the system folder and connect the
printer to the net. Then simply use the "choozer" to
locate the printer on the appropriate zone on your
networks. (This is the network connection I use)

On the PC side you will have to install the Windows
drivers and then Mount the printer as a network
printer. However, I cannot help with Novell nets..
How do you normally connect network printers?
You must have laserprinters on your Novell network and
I would guess that the procedure would be similiar.
My version of the IISDX is NOT IP aware (it's about
2 years old) so TCP/IP is not an option here.

Just a warning. Putting a dye sub on a network can
also be expensive if you have users that are not
careful. People often forget that they were
using a dye-sub printer and when they want to print
their next manuscript/email note sometimes send it to a printer assuming
they are still connected to a standard text printer,
without checking to see that the have "de-selected" the dye sub.
Hope you have some ideas on how to limit access
otherwise you may end up having lots of expensive
text printed out on your dye sub.

I use the IISDX printer for literally all my image hardcopy now.
Haven't made a print from TEM negatives in nearly
2 years. Of course, some of my instruments are
entirely digital (VG HB603Z) and hence there is no film.


Good Luck...

Nestor Zaluzec




From: {rnessler-at-emiris.iaf.uiowa.edu}:ddn:wpafb
Date: 1-20-95 8:05am
Subject: RE: Light element EDS

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Message-Id: {9501232034.AA26017-at-riker.ml.wpafb.af.mil}
To: WALCKSD:ml:wpafb, SCHELTFJ:ml:wpafb
Subj: Light element EDS
Orig-Author: {randy nessler {rnessler-at-emiris.iaf.uiowa.edu} }:ddn:wpafb
-----------------------------------------------------------

I am interested in the discussion of light element analysis using
a Kevex Quantum detector. From my discussions with Kevex, it seems that
the software available has a hard time compensating for matrix effects
when light elements are mixed with high atomic number elements. I was
wondering how other people deal with this. Our system runs on RT11, so
people who have TSX systems will have to bear with me. It appears that
the answer is standards, standards, and more standards. Let's hear it...
Randy Nessler









From: Alan Pooley :      pooley-at-ahab.rutgers.edu
Date: Mon, 23 Jan 1995 16:41:05 -0500
Subject: Re: Critical Point Drying

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An eductor tube ie a syphon tube (not ejector) is usual to get the
liquid phase of co2 out of the tube. Ris at Univ Wisc used a small
cylinder upside down, not very pratical for a large cylinder. I don't think it practical
to get the cylidar down to a low enough temp to get liquid out, I forget
the liquification temperature but it must be close to -70 C ie close to
dry ice temp? Quality is important, I have had cylinders that had water mixed with
the co2.... bad scene!! I order extra dry co2 (I believe, its written down
where I order, not here)
MOST important! be sure that the syphon/eductor tube is really there!
Test by letting gas out at a good rate, the valve should start to frost up
within 5-10 seconds at at a reasonable flow. about 20 percent of cylinders have
broken or missing eductor tubes! so beware.
A 'condenser valve' is available, I think from Fullam? (mine is ~12 years
old) this will bleed some c02 out while cooling an internal cold finger that
will recondense gaseous co2 passing through the valve to the cpd back
to liquid... provided the room temp is not too high and the cylinder is
not too empty etc.
Also only about 30 of the 50 pounds of co2 can be gotteon out as liquid
even with a condensing valve. Cold water around the chamber also helps.
Hope this helpes people.
alan pooley marine sci sem lab rutgers univ




From: John Mansfield :      John_Mansfield-at-mse.engin.umich.edu
Date: 23 Jan 1995 17:31:32 -0400
Subject: HREM: SF6 for 4000EX

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Message-ID: {n1421239928.96853-at-mse.engin.umich.edu}

John Mansfield
North Campus Electron Microbeam Analysis Laboratory
413 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (313)936-3352 FAX (313)936-3352
jfmjfm-at-engin.umich.edu or John.F.Mansfield-at-umich.edu Time: 4:46
PM

Date:1/23/95
URL: http://www.engin.umich.edu/~jfmjfm/jfmjfm.html NC EMAL

Hi there,
we are in the process of converting our JEOL 4000EX from freon to SF6 and
want to know where we can get the said gas at a reasonable price. At the
moment it looks like we are looking at between $1000 and $1700 for a single
charge and so I would appreciate it if anyone could give me pointers for a
source with a more reasonable cost
Please reply direct to me and I will summarize to the list if there is
sufficent interest.
Thanks
John Mansfield.





From: bjg-at-uniwa.uwa.edu.au (Brendon J. Griffin)
Date: Tue, 24 Jan 1995 08:09:14 +0800
Subject: Successful hookup

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Message-Id: {199501240005.IAA11949-at-uniwa.uwa.edu.au}

Ta muchly - hooked up and ready to go
Brendon J. Griffin
Centre for Microscopy and Microanalysis
The University of Western Australia
Nedlands, WA, AUSTRALIA 6907
ph 61-9-380-2739 fax 61-9-380-1087





From: Glenn Holm :      KARUZIS-at-wccf.mit.edu
Date: Mon, 23 Jan 1995 21:22:47 -0500 (EST)
Subject: lucifer yellow antibodies (light microscopy)

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we're wanting to get back into the lucifer yellow business after a
few years - filling cells in slices, observing the fills with
fluorescence, then immunostaining with antibody against LY and through
to DAB. In the past, we used a gift antibody. Are there commercial
Ab's out there that also do a good job? I have heard that there can be
some loss of fine detail in the immuno procedure, and want to know
if anyone has strong preferences as to LY antisera.

------------------------------------------------------------------
|Glenn Holm *mime mail ok* Internet:karuzis-at-wccf.mit.edu |
|M.I.T Dept. of Brain + Cog. Sci. This VAX doesn't do NeXTmail |
|Cambridge, MA 02139 "Real Neuroscientists don't do gels!" |
------------------------------------------------------------------





From: H.J. Maier :      /S=MAIER/OU=IFWT/OU=MASCHINENBAU/-at-UNI-SIEGEN.D400.de (Tel +49 271 740 2184)
Date: Tue, 24 Jan 1995 00:45:48 -0600
Subject:

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From: W.L. Steffens :      STEFFENS.B-at-calc.vet.uga.edu
Date: Tue, 24 Jan 1995 09:10:43 EST
Subject: Re: HREM- SF6 for 4000EX

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John
In my recent experience, SF6 is available from all of the major
industrial gas suppliers (ie Selox) for about the same price...~$700 per
100 lb. That was enough for about 3 charges on our JEM 1210. Your 4000
must have a BIG tank. It took about 6 weeks for our SF6 to arrive.
Obviously there is some primary supplier, but I don't know where. Good
luck!

buddy

-=W.L. Steffens=-
College of Veterinary Medicine
University of Georgia




From: swatkins-at-pitt.edu (Simon Watkins)
Date: Tue, 24 Jan 1995 09:49:14 -0500
Subject: Freeze Fracture

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A few months ago, I put a message on the board looking for a Freeze Fracture
machine, didn't get any good leads, so I thought it was time for another try:

We would like to obtain a freeze fracture machine in good working order (one
of the Balzers or Cressington machines would do fine). We have money to pay
for the machine, though not enough for a new one (sound familiar!). If you
or someone you know would be interested in selling a machine to a good home
please let me know

Thanks.
----------------------------------------------------
Simon Watkins Ph.D
Director Structural Biology Imaging Center
Scaife 840
University of Pittsburgh
Pittsburgh PA 15261

Tel:412:648-3051
Fax:412:648-1441
----------------------------------------------------





From: colijn-at-kcgl1.eng.ohio-state.edu (Henk Colijn)
Date: Tue, 24 Jan 1995 11:13:21 -0500 (EST)
Subject: Re: HREM- SF6 for 4000EX

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The price for SF6 depends greatly on the purity. Commercial purity (99.7%)
is generally available around $650-700 for a cylinder (115lb capacity).
Instrument grade (99.99%) is near $1100 per cylinder. Our prices are from
AGA (about a year ago), although I don't think you'll find significant
price differences from other vendors.

The microscope manufacturers recommend instrument grade (of course!),
although we successfully ran our H9000NAR on commercial grade.

Cheers, Henk

}
} Hi there,
} we are in the process of converting our JEOL 4000EX from freon to SF6 and
} want to know where we can get the said gas at a reasonable price. At the
} moment it looks like we are looking at between $1000 and $1700 for a single
} charge and so I would appreciate it if anyone could give me pointers for a
} source with a more reasonable cost
} Please reply direct to me and I will summarize to the list if there is
} sufficent interest.
} Thanks
} John Mansfield.

Henk Colijn colijn.1-at-osu.edu OSU Campus Electron Optics Facility
-------------------------------------------------------------------
Research Law #1
If observations disagree with theory, the observations are obviously in error.






From: Tim Foecke :      tfoecke-at-NIST.GOV
Date: Tue, 24 Jan 1995 14:21:55 -0500
Subject: TEM Opening at NIST

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Message-ID: {n1421168016.15503-at-mse.engin.umich.edu}
ONeilD-at-imb.lan.nrc.ca
X-Mailer: Mail*Link SMTP/QM 3.0.0GM

Reply to: RE} SEM-Freeze Drying equipment

I surveyed sources of freeze drying units last year when finishing up my book
on "Vacuum Methods in Electron Microscopy". At that time, these devices were
available from:
Agar Scientific (UK) Fx:0279-815106
Bal-Tech Fx: 508-374-7070
Denton Vacuum Fx: 609-424-0395
Edwards High Vacuum Fx: 505-658-7969
Electron Microscopy Sciences Fx: 215-646-8931
Emitech Fx: 713-893-8443
Energy Beam Sciences Fx;413-789-2786
Fisons FX: 415-961-8656
GeneVac (UK) FX: 0473-461176
Microfield (UK) FX:0865-821459
VG Microtech (UK) FX: 0825-768343
I don't know about the degree of automation offered, but perhaps this
will give you a start on sorting things out. Good Luck!

--------------------------------------

------------------------------------------------------------------------
| David O'Neil tel: (902) 426-8258 |
| National Research Council of Canada fax: (902) 426-9413 |
| Institute for Marine Biosciences _____ _____ |
| 1411 Oxford St. | | __/\__ | | |
| Halifax, Nova Scotia B3H 3Z1 | | __/\\ //\__ | | |
| Canada | | \ \\ // / | | |
| | | /___ ___\ | | |
| email: oneild-at-imb.lan.nrc.ca | | /__ __\ | | |
| | | || | | |
| |_____| |_____| |
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To: Microscopy-at-AAEM.AMC.ANL.GOV


This is a follow-on to the 'pre-announcement' that I posted
several weeks ago. The position is now real.

Tim Foecke


********************************************************

Materials Science and Engineering Laboratory
National Institute of Standards and Technology

We are presently expanding our capabilities within the
Materials Science and Engineering Laboratory at NIST in
the area of HREM, and have an opening for a highly
qualified Ph.D. scientist. Candidates must have extensive
hands-on experience in high resolution imaging, be well
versed in imaging and diffraction theory, and have exper-
ience in image simulation. Experience in PEELS and EDS
is essential, as is some background in materials science.
Strong interest in development and implementation of new
methodology is necessary. The position will include
extensive work with other staff members of MSEL. For
permanent employment, US citizenship is required.

Interested parties should submit a curriculum vitae
and the names of three references by MARCH 15, 1995 to:

Dr. F.W. Gayle
NIST
Building 223, Room B164
Gaithersburg, MD 20899

email: fgayle-at-nist.gov

Equal Opportunity/Affirmative Action Employer

********************************************************






From: Gib Ahlstrand :      giba-at-puccini.crl.umn.edu
Date: Tue, 24 Jan 1995 16:11:08 -0600
Subject: Ed Basgall,print prosc.

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Message-Id: {m0rWsyx-000113C-at-pegasus.cc.ucf.edu}

Ed, I tried to respond to your request for print processor information, but my
attempt bounced back with "host unknown" message. Please contact me privately
and send e-mail address and I'll try again.



--

Gib Ahlstrand, MMS Newsletter Editor
Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
495 Borlaug Hall, St. Paul, MN 55108 (612)625-8249
612-625-9728 FAX, giba-at-puccini.crl.umn.edu






From: Jan Coetzee EM Univ Pretoria :      JANC-at-ccnet.up.ac.za
Date: Wed, 25 Jan 1995 09:28:47 GMT+2
Subject: Peltier devices

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Good day all,
Thanks to all the contributors who posted (and are still posting) responses
to my question on electronic lab work scheduling. Shall try to post a
summary of the responses.

Another query:
We need to put together a cooling stage for freeze drying of small
samples. One way of doing this could be by using Peltier-effect cooling.
These devices are used in various pieces of equipment but I have not been
able to find someone who makes them. Could anyone help by supplying
references to manufacturers of Peltier-effect devices?
Thanks


Jan Coetzee
Unit for Electron Microscopy Tel:+27-12-420-2075
University of Pretoria Fax:+27-12-342-1738
Pretoria Internet:janc-at-ccnet.up.ac.za
0002 South Africa




From: Romuald Wroblewski onkpat :      Romuald.Wroblewski-at-onkpat.ki.se
Date: Wed, 25 Jan 1995 09:04:02 +0200 (METDST)
Subject: Re: SEM-Freeze Drying equipment

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I was looking for such a unit for years. I am currently using unit which
was build in our workshop for 10 years ago and still going strong. The
unit have rotary and turbo-pumps. For cooling Neslabs cryocool and liquid
nitrogen are used. Temperature of specimens down to -150C and condenser,
down to -180C. Using a data acquisition card from Strawberry Tree + Mac I
can use controll and register temperatures at 4 different sites and
register and display vacuum. Freeze-drier is used for both
SEM-preparations and Low Temperature Vacuum Embedding.
I will present the latest results from this system at SEM-meeting in
Houston, Texas in May 1995.
Costs of total unit are about USD 10 000 INCLUDING pumps, acq-card (not
crypcool).

Regards

Romuald

-------------------------
Romuald Wroblewski, Ph.D.
Associate Professor
Department of Pathology
Karolinska Institute
voice/fax:+46-8-7293597
-------------------------





From: fskarl-at-goodyear.com (Frank Karl)
Date: Wed, 25 Jan 1995 08:24:54 -0500
Subject: EDS,results and weasel words

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X-Sender: t456b15-at-rds163
Message-Id: {v01510100ab4c00843a89-at-[163.243.13.93]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Dear Folks,

I thought I would poll the EDS, WDS, EELS and other users about the proper
disclaimer to place in reports issued internally or externally.

Most of our analysis is concerned with mineral fillers and those elements
heavier than neon dispersed in an organic matrix. In some cases we will
examine the organic matrix or we ash the organic matrix and examine the
ash. The ash is either pressed on to a carbon block or on to a conductive
double-sided tape. We believe that a realistic blanket statement of
detectable for semi-quantification mode is "between neon to neptunium if
present in concetrations of approximately 0.5%" but we feel a need to add
some weasel words about the sample.

The question: Which best describes the limitations of our analysis by our
samples? 0.5% by weight or 0.5% by volume, or is there a better phrase?
Or are we just fooling ourselves?

Thanks........Frank


Frank Karl fskarl-at-goodyear.com
Goodyear Tire & Rubber Co. Voice 216.796.7818
Analytical Services - Dept 415B Fax 216.796.3304
142 Goodyear Blvd
Akron, OH 44305
U.S.A.









From: MOLE:: fskarl-at-goodyear.com 25-JAN-1995 07:27:47.64
Date: Wed, 25 Jan 1995 08:31:37 -0600
Subject: EDS results & weasel words

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Dear Folks,

I thought I would poll the EDS, WDS, EELS and other users about the proper
disclaimer to place in reports issued internally or externally.

Most of our analysis is concerned with mineral fillers and those elements
heavier than neon dispersed in an organic matrix. In some cases we will
examine the organic matrix or we ash the organic matrix and examine the
ash. The ash is either pressed on to a carbon block or on to a conductive
double-sided tape. We believe that a realistic blanket statement of
detectable for semi-quantification mode is "between neon to neptunium if
present in concetrations of approximately 0.5%" but we feel a need to add
some weasel words about the sample.

The question: Which best describes the limitations of our analysis by our
samples? 0.5% by weight or 0.5% by volume, or is there a better phrase?
Or are we just fooling ourselves?

Thanks........Frank


Frank Karl fskarl-at-goodyear.com
Goodyear Tire & Rubber Co. Voice 216.796.7818
Analytical Services - Dept 415B Fax 216.796.3304
142 Goodyear Blvd
Akron, OH 44305
U.S.A.









From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Wed, 25 Jan 1995 9:50:38 -0600 (CST)
Subject: Test Messages

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X-Nupop-Charset: English


G'day Subscribers....

Just a note. It is clearly inappropriate to
use the Microscopy Listserver to send out Test
messages to see if your Email server is running.
There are somewhere over 2000 people receiving
these messages please be intelligent. If you are
having an Email problem then send a message to
only one person. If you are really in a bind then
just send a message to me and I will respond when
I get a chance, but not to the world!!

Nestor
Your Friendly (& Lately Tired) Neighborhood SysOp




From: Greg Erdos ICBR EM Core Lab Univers :      GWERDOS-at-gnv.ifas.ufl.edu
Date: Wed, 25 Jan 1995 11:29:25 -0500 (EST)
Subject: Biol. SEM Textbook

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I am planning to teach a short course in biological SEM for novices and
need to find a textbook for the students. I am considering Postek et al., 1980
published by Ladd. Would any of you out there recommend anything more recent
that would be appropriate for grad students, staff, faculty, post-docs.
Any input willl be appreciated.

Although my course is intended for local consumption, iot would be
open to anyone, so if there are interested parties wanting to spend a week in
Florida in May, just E- mail me at the address below

Thanks
******************************************************
* Greg Erdos ** *
* Director, ICBR EMCL ** Phone 904-392-1295 *
* 218 Carr Hall ** FAX 904-846-0251 *
* University of Florida ** gwerdos-at-gnv.ifas.ufl.edu *
* Gainesville, FL 32611 ** *
******************************************************************




From: sc_marschman-at-ccmail.pnl.gov
Date: Mon, 23 Jan 1995 09:39 -0800 (PST)
Subject: Need Help Developing Optical Techniques for Uranium Hydride

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I posted this note yesterday on several newsgroups, but
just found out about this listserver today (1-25-95).
I am looking for some help...

My group is responsible for examining uranium-metal
spent nuclear fuel (N-Reactor, Single-Pass Reactors)
which has degraded over time while in water storage at
the Hanford, Washington site. There is a possibility
that some of the uranium may have reacted with water to
form uranium hydride which still may be present in some
of the fuel. One of our tasks is to determine if this
scenario is real. Thus, we will be examining several
fuel elements to look for evidence of hydrides. We
have (and will use) optical microscopy and electron
microscopy (AFM, SEM, TEM) in our efforts.

Because the fuel is highly radioactive, and has a
potential for pyrophoric reactions, our first efforts
will be to cut, section, polish, and optically examine
the fuel in a radiation hot cell using an argon cover
gas blanket over all operations. However, when we get
to the point of specifying etchants or etching
techniques to examine the uranium, no one is sure how
best to bring out the features of uranium hydride in
uranium metal. We will try cathodic etching first,
largely because this is what has been traditionally
done to examine uranium at our lab. However, we aren't
sure what to expect from the hydride (if it exists).
Our literature searches have drawn a blank with regard
to microscopy techniques for uranium hydrides.

So, I would like to ask, has anyone had experience
optically examining uranium hydrides? If so, could you
recommend a procedure? We also have the possibility of
hiring a consultant if the right person or lab anywhere
in the world) is identified to assist us.

Thanks
Steve Marschman
sc_marschman-at-pnl.gov




From: :      MELLIOTT-at-prl.pulmonary.ubc.ca
Date: Wed, 25 Jan 1995 13:33:15 +0800PST
Subject: lm-quantifying immunolabelling

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I have a question for those people involved in light microscopic
immunolabelling and trying to quantify the amount of stainig. Can
anyone do it reliably?? We have someone in the lab who wants to
determine if the staining obtained with 6 different antibodies
co-localize within the tissue. They are staining paraffin-embedded
sections and are staining one antibody/section. They want to know if
there is a way to do this using an imaging system of some sort. IMHO
I don't thinnk it can be done easily. If anyone has any
trhoughts/ideas they would be GREATLY APPRECIATED.

Thanks
Mark Elliott, PhD
Pulmonary Research Lab
UBC
Vancouver
Canada




From: X.m. Burany :      burany-at-sfu.ca
Date: Wed, 25 Jan 1995 13:58:54 -0800 (PST)
Subject: REM

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Hi Jeff:

We do some reflection diffraction electron microscopy. Would you
please let us know more details of your work on REM.

Sandy Burany
Dept. of Physics
Simon Fraser University
Burnaby, B.C. V5A 1S6
Canada
(604) 291-4082
Fax (604) 291-3592
Email xm_burany-at-sfu.ca





From: M.V. Parthasarathy :      mvp2-at-cornell.edu
Date: Wed, 25 Jan 1995 18:46:13 -0400 (EDT)
Subject: RE: lm-quantifying immunolabelling

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X-NUPop-Charset: English

Quantitative fluorescence microscopy can be tricky. Please refer to the
chapter by Jesse E. Sisken (Fluorescence Standards) in Methods in Cell Biology, vol. 30, edited by
L.Taylor and Y. Wang (1989; Academic Press) for recommendations.

*************************************************************************
M.V. Parthasarathy
Professor of Plant Biology
Section of Plant Biology
228 Plant Science Building
Cornell University, Ithaca, NY 14850
Telephone: (607) 255-1734
Fax: (607) 255-5407
E-mail: mvp2-at-cornell.edu
***********************************************************************




From: Michael Rock :      merock-at-u.washington.edu
Date: Wed, 25 Jan 1995 17:10:31 -0800 (PST)
Subject: Re: Conductive glue needed!

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Try using silver paint, we use it & it seems to be working well.
-Mike

On Wed, 25 Jan 1995 tsu-at-cae.wisc.edu wrote:

}
} Hi, microscopist,
}
} Does anyone know if there's any conductive glue by which we can attach
} our TEM samples to Cu/Au grids? M-bond is what I currently use and has
} been suspected to cause some shifting of images especially for tiny
} samples. Any information or comment will be appreciated.
}
} I-Fei Tsu
} ASC-MRG
} UW-Madison
}
}




From: Weimin Tao :      wtao-at-mtu.edu
Date: Wed, 25 Jan 1995 20:58:07 -0500
Subject: Re: Conductive glue needed!

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subscribe weimin tao







From: {tsu-at-cae.wisc.edu}:ddn:wpafb
Date: 1-25-95 3:56pm
Subject: Conductive glue needed!

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Message-Id: {9501261225.AA04965-at-riker.ml.wpafb.af.mil}
To: WALCKSD:ml:wpafb, SCHELTFJ:ml:wpafb
Subj: Conductive glue needed!
Orig-Author: {tsu-at-cae.wisc.edu}:ddn:wpafb
-----------------------------------------------------------

Hi, microscopist,

Does anyone know if there's any conductive glue by which we can attach
our TEM samples to Cu/Au grids? M-bond is what I currently use and has
been suspected to cause some shifting of images especially for tiny
samples. Any information or comment will be appreciated.

I-Fei Tsu
ASC-MRG
UW-Madison





From: schwartz-at-mrvx03.mdc.com
Date: Thu, 26 Jan 1995 08:41:26 -0600
Subject: Electropolishing Mo-Re

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My Fellow Microscopists:

I am trying to prepare TEM thin foils from Mo-47.5Re (at.%) using a
twin-jet electropolisher, without success. I have tried cold sulfuric
acid/methanol solutions and perchloric acid/methanol solutions. These
produce a pebbly surface with minimal electron transparent areas.

Does anyone out there have experience producing TEM thin foils from
Mo-Re? I would greatly appreciate any suggestions!
_ _
O-O
J (a sideways smiley) Thanks, Dan Schwartz
~





From: South Bay Technology :      73531.1344-at-compuserve.com
Date: 26 Jan 95 12:56:27 EST
Subject: Electropolishing Mo-Re

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I spoke to Bernie Kestel at Argonne National Lab and he gave the following
solution that has worked well for him:

Material: Mo 30a/o Re
Equipment: South Bay Technology Model 550 Single Vertical-Jet
ElectroPolisher
Electrolyte: 12% Sulphuric Acid
83% Methanol
5% Butyl Cellosolve
Temperature: -50C
Voltage: 190V
Current: 50-75 ma
Pump Speed: Slow

Since the work was done with a SBT Single Jet System, he suggested that you
change the temperature to perhaps -40C and use a somewhat lower voltage for a
twin jet system.

Of course, my solution would be to buy a South Bay unit, but I do have a vested
interest in that! We also publish a bibliography of technical reports on sample
preparation and distribute copies of the papers free of charge. Most of the
reports deal with TEM sample preparation - primarily electropolishing, dimpling
and Tripod Polishing.

Best regards-

David Henriks
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673

TEL: 800-728-2233
FAX: 714-492-2600





From: {schwartz-at-mrvx03.mdc.com}:ddn:wpafb
Date: 1-26-95 10:39am
Subject: Electropolishing Mo-Re

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Message-Id: {m0rXYnF-0000zMC-at-pegasus.cc.ucf.edu}
To: WALCKSD:ml:wpafb, SCHELTFJ:ml:wpafb
Subj: Electropolishing Mo-Re
Orig-Author: {schwartz-at-mrvx03.mdc.com}:ddn:wpafb
-----------------------------------------------------------
My Fellow Microscopists:

I am trying to prepare TEM thin foils from Mo-47.5Re (at.%) using a
twin-jet electropolisher, without success. I have tried cold sulfuric
acid/methanol solutions and perchloric acid/methanol solutions. These
produce a pebbly surface with minimal electron transparent areas.

Does anyone out there have experience producing TEM thin foils from
Mo-Re? I would greatly appreciate any suggestions!
_ _
O-O
J (a sideways smiley) Thanks, Dan Schwartz
~






From: emlab-at-ucsco.ucsc.edu (Jon Krupp)
Date: Thu, 26 Jan 1995 13:42:12 -0800
Subject: EM embedding

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Greetings:

This may be a simple minded question, but I could use some advice. I just
mixed some Epon Araldite type embedding plastic and it got really dark in
color after adding the BDMA. I was using an Epon 812 substitute, DDSA,
Araldite 502, and BDMA. It is a slightly different recipe than we have used
before and our plastic is usually a pleasant golden color, this stuff was
like dark caramel in color. The color came from the interaction of the BDMA
and DDSA, or at least that is the way it looked after mixing the BDMA with
the other components individually.

Does anyone have a suggestion to explain the dark color and any free advice
about this phenomenon in general.

In a related question, can anyone say what the useful shelf lives of the
various embedding components might be. Some of ours are older, someone buys
a lot and never uses it up, should we toss everything and start over or can
we hold on and use what we have?

Thanks for your help.


Jonathan Krupp
Microscopy and Imaging Lab
University of California
Santa Cruz, CA 95060
emlab-at-ucsco.ucsc.edu
(408) 459-2477






From: hukee.margaret-at-mayo.EDU (Marge Hukee)
Date: Thu, 26 Jan 1995 18:07:03 +0200
Subject: Re: EM embedding

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Message-Id: {9501270004.AA26961-at-fermat.Mayo.EDU}
X-Sender: hukem-at-fermat.mayo.edu
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

JON:

I DON'T THINK THE AGE OF YOUR RESIN COMPONENTS HAS ANY INFLUENCE ON THE
DARK COLOR. OUR RESIN BATCHES ARE TURNED-OVER RAPIDLY, AND WE HAVE NOTICED
A DARK COLOR IN OUR SPURR IN COMPONENTS THAT WERE RECENTLY ACQUIRED. WE
CALLED ONE OF THE SUPPLY COMPANIES AND WERE TOLD THAT AN ALTERED PH IN ONE
OF THE COMPONENTS WAS RESPONSIBLE. SINCE OUR SPURR RECIPE AND YOUR RESIN
RECIPE DO NOT HAVE ANY COMPONENTS IN COMMON, PERHAPS THIS IS A GENERAL
RESIN PROBLEM. ANY COMMENTS FROM RESIN ENGINEERS?

MARGE

Marge Hukee
EM Core Facility hukee.margaret-at-mayo.edu
Mayo Foundation (507) 284-3148
----------------------------------------------------------------------------
--






From: Ron Oldfield (roldfiel-at-rna.bio.mq.edu.au)
Date: Fri, 27 Jan 1995 14:11:56 GMT+1100
Subject: Re:unsubscribe

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Date sent: 27 January 1995

About to enjoy a break.....please unsubscribe, and thanks.




Ron Oldfield
School of Biological Sciences
Macquarie University
NSW Australia 2109
email: roldfiel-at-rna.bio.mq.edu.au
phone: (612) 850 8173
fax: (612) 850 8116







From: SveEn-at-pai.liu.se (Sverker Enestrom)
Date: Fri, 27 Jan 1995 08:26:01 +0100
Subject: LM-quantifying immunolabeling

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Mats Karlsson (at Dept. of Pathology, Orebro Medical Center Hospital,
Orebro, Sweden)
et al. have recently described the methodological approach to quantify
immunostained
objects in histological sections by computerized image analysis in:
Pathology, Res. and
Pract. 1995 (in press), using frozen sections. Intra- and interindividual
variations were
less than 5% and the correlation of reproducibility was high.
If you are interested, contact me directly.

==================================================================
Sverker Enestrom M.D., Ph.D.
Department of Pathology
University of Linkoping, Sweden
Phone: +46 13 22 15 20
Fax: +46 13 13 22 57
==================================================================






From: Peter Makroczy RNDr. :      makroczy-at-ccsun.tuke.sk
Date: Fri, 27 Jan 1995 09:06:02 +0100 (MET)
Subject: Channeling patterns on TEM

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Does anyone has experience how to get channeling patterns on JEOL Jem 2000FX
electron microscope equipped with ASEA20 in STEM/BEI mode? Please some
hints, advices.

Thank You.

Peter Makroczy
Technical University of Kosice
Dept.of Materials Science
Park Komenskeho 11
043 85 Kosice
Slovak republic

E-mail: makroczy-at-ccsun.tuke.sk




From: {schwartz-at-mrvx03.mdc.com}:ddn:wpafb
Date: 1-26-95 10:39am
Subject: Re: Electropolishing Mo-Re

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Message-Id: {9501271212.AA08568-at-riker.ml.wpafb.af.mil}
To: WALCKSD:ml:wpafb, SCHELTFJ:ml:wpafb
Subj: Electropolishing Mo-Re
Orig-Author: {schwartz-at-mrvx03.mdc.com}:ddn:wpafb
-----------------------------------------------------------
My Fellow Microscopists:

I am trying to prepare TEM thin foils from Mo-47.5Re (at.%) using a
twin-jet electropolisher, without success. I have tried cold sulfuric
acid/methanol solutions and perchloric acid/methanol solutions. These
produce a pebbly surface with minimal electron transparent areas.

Does anyone out there have experience producing TEM thin foils from
Mo-Re? I would greatly appreciate any suggestions!
_ _
O-O
J (a sideways smiley) Thanks, Dan Schwartz
~



--Boundary (ID 6KnNZ50yHI4SNRb1ETY+XQ)





From: Peter Makroczy RNDr. :      makroczy-at-ccsun.tuke.sk
Date: Fri, 27 Jan 1995 13:28:36 +0100 (MET)
Subject: Channeling patterns on TEM

Contents Retrieved from Microscopy Listserver Archives
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Does anyone has experience how to get channeling pattern on JEOL Jem 2000FX
electron microscope equipped with ASEA 20 in STEM/BEI mode. Please some
hints, advices.

Thank You.

Peter Makroczy
Technical University of Kosice
Dept. of Materials Science
Park Komenskeho 11
043 85 Kosice
Slovak republic

E-mail: makroczy-at-ccsun.tuke.sk




From: EMLAB-at-opus.mco.edu
Date: Fri, 27 Jan 1995 08:39:45 -0400 (EDT)
Subject: Re: EM embedding

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Jonathon,

Way back when, I was using EPON-ARALDITE resin components that were at least
10 years old at the time. No problems with using these. I do not recall
having any problems with a dark color. What % of BDMA are you using? I
used 2%(v/v). The only problem I can forsee in using a dark batch of resin
would be that your background of your sections will not be as light as they
should be. Best of luck.

Ed Calomeni




From: Herbert K. Hagler, Ph.D. :      hagler-at-UTSW.SWMED.EDU
Date: Fri, 27 Jan 1995 08:01:13 +0800 (U)
Subject: Re: LM-Quantifying Immunolabelling

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Monica {NASSM-at-QUCDN.QueensU.CA}
Message-id: {01HMC1146R5U8ZUHIB-at-UTSW.SWMED.EDU}
X-Mailer: Mail*Link PT/Internet 1.5.1
Content-transfer-encoding: 7BIT

It is hard to understand why people are still attempting to 'quantitate'
imunohistochical reactions which rely on a final step in which the 'quantity'
of stain is not related to the amount of antigen present. In order to
quantitate anything there has to be a quantitative relationship which can be
measured relating what is seen in the section to the amount of material
detected. In other words you cannot quantiate the standard immunoperoxidase
techniques used in immunocytochemistry. This does not negate their
importance, but means that one should not attempt to do image analysis on
this material.

For a complete discussion of these limitations see the bottom of page 6 in
the Gareth Griffiths book on "Fine Structure Immuno-cytochemistry,
Springer-Verlag, 1993, ISBN 3-540-54805-X.
The following is a quote from this page "..., Fig 1 shows a striking example
of a little appreciated fact, namely, how the immunoperoxidase method can
often give results that, though qualitatively correct, may be quantitatively
misleading.
Most people who have worked extensively with any of the HRP techniques become
aware of these problems and may be frustrated by the presence of many
variables that cannot, even empirically, be completely controlled. Even if
only qualitative data are required, it is very difficult in practice to find
the right compromise between acceptable fine structure preservation and
specific labelling. This does not belittle the historical importance of the
immunoperoxidase techniques..."


Herbert K. Hagler, Ph.D.
Microscopy and Imaging Service Center
Dept of Pathology, UT Southwestern Medical Center
5323 Harry Hines Blvd., Dallas, TX 75235-9072
phone (214) 648-3890 fax (214) 648-3925




From: Giovanni Valdre' :      gvaldre-at-dogon.geomin.unibo.it
Date: Fri, 27 Jan 1995 17:34:16 +0000
Subject: unsubscribe

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Message-Id: {9501271638.AA09836-at-Dogon.geomin.unibo.it}
Sender: {gvaldre-at-dogon.geomin.unibo.it}

please, unsubscribe
-------------------------------------------
Giovanni Valdre'
Dipartimento Di Scienze Mineralogiche
Piazza di Porta S. Donato 1
I-40126 Bologna, Italy
Tel. +39 51 243556 FAX +39 51 243336
email gvaldre-at-geomin.unibo.it
-------------------------------------------





From: Mriglermas-at-aol.com
Date: Fri, 27 Jan 1995 16:12:41 -0500
Subject: temhelp

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To whom it may concern:

Please send copies of Temhelp letters or questions to me. We perform all
types of EM analyses and can probably help with a variety of
techniques/comments.

Thank you very much.


Regards,


Mark W. Rigler, Ph.D.
Materials Analytical Services
Norcross, Georgia
1800-421-8451




From: Glen Macdonald :      glenmac-at-u.washington.edu
Date: Fri, 27 Jan 1995 22:14:42 -0800 (PST)
Subject: Re: EM embedding

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Many different epoxies from various manufacturers, with widely varying
ages have all given me exceptionally dark pot mixtures from time to
time. There are only two things that I've been able to narrow it down to.
One is accelerator, BDMA, DMAE or DMP-30 that has been opened too long.
Water contamination has been suggested by mfrs. I now toss the
accelerator when a bottle of epoxy (Epon, DER 736, etc.) is emptied.
Some suppliers are worse than others. We quit dealing with Polysciences
entirely, because of irregularities in the final blocks. (color is minor,
it's when things won't get hard, and kits arrive missing components that
things are serious).
The other correlation on block color is delay in mixing. If you delay,
you get coloration. Delay too long, or mix with a stir bar so slowly
that you get layer formation, and the upper layer will get very dark and
form lumps.
Just my $.02.

Glen MacDonald
Hearing Development Laboratories RL-30
University of Washington
Seattle, WA 98195
(206)543-8360
glenmac-at-u.washington.edu






From: Norm Granholm :      Norman.Granholm-at-UC.Edu
Date: Sat, 28 Jan 1995 11:27:23 -0500 (EST)
Subject: 2D software for Unix platform

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I ask for comments, recommendations, advice, for 2D image analysis software
for a UNIX platform (SGI Indy R4000).

If there is interest I'll post a summary. Thanks.

Norm Granholm, Pathology, Univ. Cincinnati College Medicine
(granhona-at-ucbeh.san.uc.edu)
V: 513 558 0182





From: Alan Partridge :      alan-at-surf.phys.tue.nl
Date: Mon, 30 Jan 1995 11:15:34 +0100 (MET)
Subject:

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Message-Id: {199501301017.LAA28059-at-mailhost.tue.nl}

Subscribe





From: Alan Partridge :      alan-at-surf.phys.tue.nl
Date: Mon, 30 Jan 1995 13:26:15 +0100 (MET)
Subject: subscribe

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Message-Id: {199501301227.NAA01841-at-mailhost.tue.nl}

Subscribe





From: Marcelle A Gillott :      magem-at-csd.uwm.edu
Date: Mon, 30 Jan 1995 08:43:45 -0600 (CST)
Subject: cryo-ultramicrotomy equipment

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looking for feedback (mostly negative) from users - I have demo'd the
systems and have a few "satisfied customer" refs from sales and of course
each has annon. "horror stories" about the competition
Hi everyone

I am in the process on deciding on a purchase of cryo-fixation and
ultramicrotomy equipment and would appreciate some feedback from users -

I have demo'd both the Reichert Ultracut S and the RMC 7000 with their
cryo systems and they seem pretty comparable, each having its own
plusses and minuses -

This instrument will be used for both teaching and research - also it
will msot likey be a very long time before we purchase another one

I would appreciate any positive and/or NEGATIVE comments you might have
on these instruments - particularly your experience regarding reliability
and service and support after the sales (I will not be taking out a
service contract)

We are also contemplating the purchase of a jet freezer from either
Bal-Tec or RMC (they bought out the Life-cell system) & would appreciate
any comments from users about those instruments as well

Responses which are sent directly to me (as opposed to those posted to
the list) will remain confidential

Thanks


Marcelle Gillott, Ph.D.
Director, EM Laboratory
University of Wisconsin-Milwaukee

magem-at-csd.uwm.edu

PH: 414-229-4186





From: rms-at-vax.ox.ac.uk
Date: Mon, 30 Jan 1995 16:01:37 +0000
Subject: Journal of Microscopy, December 1994 - February 1995

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Journal of Microscopy, Vol. 176, Pt. 3, December 1994, pp.
181-187.

In situ analysis of microbial consortia in activated sludge
using fluorescently-labelled, rRNA-targeted oligonucleotide
probes and scanning confocal laser microscopy

M. Wagner, B. Assmus, A. Hartmann, P. Hutzler & R. Amann
Lehrstuhl fur Mikrobiologie, Technische Universitat Munchen,
Arcisstrasse 21, D-80290 Munchen, Germany


SUMMARY

Activated sludge flocs are complex consortia of various micro-
organisms. The community structures of samples taken from
municipal sewage treatment plants were characterized using
fluorescently labelled, 16S and 23S rRNA-targeted
oligonucleotide probes in combination with confocal scanning
laser microscopy (CSLM). In comparison with conventional
epifluorescence microscopy, CSLM considerably improved the
capability to visualize directly the spatial distribution of
defined bacterial populations inside the sludge flocs.
Analyses could be performed at high resolution undisturbed by
problems such as autofluorescence or limited spatial
resolution in thick samples. In addition, CSLM was used to
analyse some structural properties of paraformaldehyde-fixed
activated sludge flocs, such as floc size and homogeneity.
Typical floc sizes were found to be in the range between 5 and
50 micrometre. Whereas most of the flocs were completely
colonized by bacteria, there were also examples of flocs
containing gas bubbles or particles in the interior.


Journal of Microscopy, Vol. 176, Pt. 3, December 1994, pp.
188-194.

Scanning interference and confocal microscopy

R. Juskaitis & T. Wilson, Department of Engineering Science,
University of Oxford, Parks Road, Oxford, OX1 3PJ, U.K.


SUMMARY

The form of the interference term image in scanning confocal
and scanning conventional interference microscopes is
identical in all respects including optical sectioning. This
observation is used to obtain confocal images and surface
profiles from conventional scanning interference microscope
images.


Journal of Microscopy, Vol. 176, Pt. 3, December 1994, pp.
195-203.

Time- and wavelength-resolved spectroscopy in two-photon
excited fluorescence microscopy

S. Andersson-Engels, I. Rokahr & J. Carlsson
Department of Physics, Lund Institute of Technology, PO Box
118, S-221 00 Lund, Sweden


SUMMARY

Two-photon excited fluorescence spectroscopy has been
performed at a microscopic scale in combination with normal,
white light microscopy. This gave simultaneously a spectral
resolution of 20nm and a temporal resolution of 20ps, from a
volume element less than 5 micrometre in all three dimensions.
The sample was excited with light from a continuously mode-
locked Ti:sapphire laser that was focused on the sample in a
fluorescence microscope. A polychromator and streak-camera
were used for detection. The method has been used on tissue,
plant and paper samples. It has also been demonstrated how
substances naturally occurring in the samples can be
identified from their spectroscopic properties and the spatial
distribution of these substances can be observed.



Journal of Microscopy, Vol. 176, Pt. 3, December 1994, pp.
204-210.

Intracellular localization of the antitumour drug adriamycin
in living cultured cells: a confocal microscopy study

S. Meschini, A. Molinari, A. Calcabrini, G. Citro & G. Arancia
Department of Ultrastructures, Istituto Superiore di Sanita,
Viale Regina Elena 299, 00161 Rome, Italy


SUMMARY

The intracellular distribution of the anthracyclinic
antibiotic adriamycin in living cultured cells has been
investigated by confocal microscopy.
In human melanoma cells (M14), adriamycin was localized
inside the nuclei. When adriamycin-treated M14 cells were
allowed to recover in a drug-free medium, a complete efflux of
the drug from the nucleus was revealed. In recovered cells, a
weakly fluorescent signal was observed in the perinuclear
region. When M14 cells were recovered in a medium containing
colcemid, a microtubule depolymerizing agent, the drug
transport from the nucleus to the cell periphery appeared to
be inhibited, suggesting that the microtubule network is
strongly involved in drug transport mechanisms. In multidrug-
resistant (MDR) cells the intracellular location of adriamycin
was shown to be noticeably different from that of the parental
wild-type cells. In particular, in resistant human breast
carcinoma cells (MCF-7), adriamycin appeared to be exclusively
located within the cytoplasm, whereas the nuclei were shown to
be completely negative. When adriamycin treatment was
performed in association with MDR revertants, such as
Lonidamine (inhibitor of the energy metabolism) or verapamil
(inhibitor of the P-glycoprotein efflux pump), a marked
enhancement of the cytoplasmic signal was observed in
resistant cells. Under these conditions, adriamycin appeared
concentrated in the perinuclear region, but the nuclei were
still negative. Confocal microscopy proved to be a useful
method for the study of the intracellular transport of
fluorescent substances, such as anthracyclinic antibiotics,
and for the investigation of the multidrug resistance
phenomenon in tumour cells.



Journal of Microscopy, Vol. 176, Pt. 3, December 1994, pp.
211-221.

A versatile tilting device for fluorescence microscopes

J. Bradl, M. Hausmann, B. Schneider, B. Rinke & C. Cremer
Institut fur Angewandte Physik, Albert-Uberle Strasse 3-5,
D-69120 Heidelberg, Germany


SUMMARY

A tilting device for biological specimens (rotation angle up
to 2 pi) especially fluorescence-labelled cell nuclei, was
developed. It consists of a quartz glass capillary and a
mounting adaptor for the microscope stage. The applicability
of the device was tested for several epifluorescence and
confocal scanning laser microscopes. The axis of rotation is
perpendicular to the optical axis of the microscope. The
capillary can be tilted around its axis at any desired angle
or in equiangular steps. This can be done manually or by
remote control using a stepping motor.
The three-dimensional (3-D) image-forming properties of
the capillary system were experimentally examined using an
inverse confocal scanning laser microscope. The results were
compared with measurements obtained from the same microscope
with the standard stage for plane slides with cover glasses.
The measured point spread function suggested that, in spite of
aberration effects, the optical arrangement used allows a gain
in the 3-D resolution by tilting the object.
A low-cost, fully-automated 3-D imaging system was built
on the basis of a conventional epifluorescence microscope with
a cooled black-and-white CCD camera. The system was operated
by a personal computer. The online visualization ('movie') of
rotating objects indicates the feasibility of the system.



Journal of Microscopy, Vol. 176, Pt. 3, December 1994, pp.
222-225.

Continuous wave excitation two-photon fluorescence microscopy

P. E. Hanninen, E. Soini & S. W. Hell
Department of Medical Physics, University of Turku, Center for
Biotechnology, Tykistokatu 6, FIN-20521 Turku, Finland


SUMMARY

Two-photon excitation fluorescence imaging is feasible with
continuous wave lasers. Images of biological specimens are
obtained by employing photon counting in conjunction with an
increasing recording time. The approach allows two-photon
three-dimensional imaging of fluorescently-labelled specimens
with inexpensive lasers.


Journal of Microscopy, Vol. 176, Pt. 3, December 1994, pp.
226-230.

Refractive-index-induced aberrations in two-photon confocal
fluorescence microscopy

H. Jacobsen, P. E. Hanninen, E. Soini & S. W. Hell
Department of Medical Physics, University of Turku, Center for
Biotechnology, Tykistokatu 6, FIN-20521 Turku, Finland


SUMMARY

The effect of refractive index mismatch on the image quality
in two-photon confocal fluorescence microscopy is investigated
by experiment and numerical calculations. The results show a
strong decrease in the image brightness using high-aperture
objectives when the image plane is moved deeper into the
sample. When exciting at 740nm and recording the fluorescence
around 460nm in a glycerol-mounted sample using a lens of a
numerical aperture of 1.4 (oil immersion), a 25% decrease in
the intensity is observed at a depth of 9 micrometre. In an
aqueous sample, the same decrease is observed at a depth of 3
micrometre. By reducing the numerical aperture to 1.0, the
intensity decrease can be avoided at the expense of the
overall resolution and signal intensity. The experiments are
compared with the predictions of a theory that takes into
account the vectorial character of light and the refraction of
the wavefronts according to Fermat's principle. Advice is
given concerning how the effects can be taken into account in
practice.


Journal of Microscopy, Vol. 176, Pt. 3, December 1994, pp.
231-237.

The tetrahedral tip as a probe for scanning near-field optical
microscopy at 30nm resolution

U. C. Fischer, J. Koglin & H. Fuchs
Westfalische Wilhelms Universitat, Physikalisches Institut,
Wilhelm-Klemm-Strasse 10, 48149 Munster, Germany


SUMMARY

The tetrahedral tip is introduced as a new type of probe for
scanning near-field optical microscopy (SNOM). Probe
fabrication, its integration into a scheme of an inverted
photon scanning tunnelling microscope and imaging at 30nm
resolution are shown. A purely optical signal is used for
feedback control of the distance of the scanning tip to the
sample, thus avoiding a convolution of the SNOM image with
other simultaneous imaging modes such as force microscopy. The
advantages of this probe seem to be a very high efficiency and
its potential for SNOM at high lateral resolution below 30nm.



Journal of Microscopy, Vol. 176, Pt. 3, December 1994, pp.
238-244.

Studies of porphyrin containing specimens using an optical
spectrometer connected to a confocal scanning laser microscope

O. Trepte, I. Rokahr, S. Andersson-Engels & K. Carlsson
Physics IV, The Royal Institute of Tech, S-100 44 Stockholm,
Sweden


SUMMARY

A spectrometer has been developed for use with a confocal
scanning laser microscope. With this unit, spectral
information from a single point or a user-defined region
within the microscope specimen can be recorded. A glass prism
is used to disperse the spectral components of the recorded
light over a linear CCD photodiode array with 256 elements. A
regulated cooling unit keeps the detector at 277K, thereby
allowing integration times of up to 60s. The spectral
resolving power ranges from 350 at 400nm to 100 at 700nm.
Since the entrance aperture of the spectrometer has the same
size as the detector pinhole used during normal confocal
scanning, the three-dimensional spatial resolution is
equivalent to that of normal confocal scanning. Light from the
specimen is deflected to the spectrometer by a solenoid
controlled mirror, allowing fast and easy switching between
normal confocal scanning and spectrometer readings.
With this equipment, studies of rodent liver specimens
containing porphyrins have been made. The subcellular
localization is of interest for the mechanisms of photodynamic
therapy (PDT) of malignant tumours. Spectroscopic detection is
necessary to distinguish the porphyrin signal from other
fluorescent components in the specimen. Two different
substances were administered to the tissue, Photofrin, a
haematoporphyrin derivative (HPD) and delta-amino levulinic
acid (ALA), a precursor to photoporphyrin IX and haem in the
haem cycle. Both are substances under clinical trials for PDT
of malignant tumours. Following administration of these
compounds to the tissue, the potent photosensitizer and
fluorescent compound photofrin, or protoporphyrin IX,
respectively, is accumulated. For our study Wistar/Furth rats
were injected either with Photofrin or with ALA 3-5h before
they were killed. The organs were removed directly after and
snap-frozen in carbon dioxide ice with isopentane. No further
staining or fixation procedures were adopted.


Journal of Microscopy, Vol. 176, Pt. 3, December 1994, pp.
245-253.

Modelling of inclined and curved surfaces in the reflection
scanning acoustic microscope

W. Weise, P. Zinin & S. Boseck
Institute for Materials Science and, Structure Research,
Physics Department, University of Bremen, 28334 Bremen,
Germany


SUMMARY

An expression is derived for the output signal when an
inclined plane surface is imaged by the reflection scanning
acoustic microscope, which is modelled as a spherical
transducer. This expression is applied to model non-planar
surfaces. The accuracy of this approach is tested for
perfectly reflecting spherical surfaces. The influence of
inclination on V(z) curves is considered when Rayleigh waves
occur.


Journal of Microscopy, Vol. 176, Pt. 3, December 1994, pp.
254-261.

Scanning force microscopy on live cultured cells: imaging and
force-versus-distance investigations

D. Ricci & M. Grattarola
Dipartimento di Ingegneria Biofisica, ed Elettronica,
Universita degli Studi di Genova, Via Opera Pia 11a, 16145
Genova, Italy


SUMMARY

Extensive measurements with the scanning force microscope
(SFM) on living cells in their native liquid environment are
described with the purpose of critically assessing the extent
of the interaction between the SFM tip and the (soft) cell
materials and the effect of such interaction on topographic
information. Images are obtained under various force
conditions and systematically correlated with force-versus-
distance curves. As a result, detailed indications about tip
indentation are given, thickness estimates deduced and
identification of submembranous cytoplasmic structures
suggested.


Journal of Microscopy, Vol. 176, Pt. 3, December 1994, pp.
262-275.

In vivo analysis of angiogenesis and revascularization of
transplanted pancreatic islets using confocal microscopy

F. A. Merchant, S. J. Aggarwal, K. R. Diller & A. C. Bovik
Biomedical Engineering Research Program, ENS 612, University
of Texas, Austin 78712-1084, U.S.A.


SUMMARY

A technique to measure angiogenesis and revascularization in
pancreatic islets transplanted at the renal subcapsular site
in the rat has been developed. In vivo imaging of the
microcirculation of transplanted pancreatic islets was
conducted using a confocal scanning laser microscope (CSLM) to
achieve optical sectioning through the graft in order to
perform a computer reconstruction of the three-dimensional
neovascular morphology. Individual islets were harvested by
enzymatic digestion of excised pancreas from Fischer 344 rats.
Isolated islets were cultured for 24h, and approximately 300-
350 islets were transplanted at the renal subcapsular site of
the left kidney in an anaesthetized rat. Six to 14 days post-
transplantation, the animal was anaesthetized and prepared for
in vivo imaging of the microvasculature on a Zeiss LSM-10.
Optical contrast of the microvasculature was enhanced by the
administration of fluorescein-labelled dextran into the
circulating blood. The transplant site was identified and
serial sections were obtained through the vascular bed at
varying z-intervals. Complementary fluorescence video images
were also obtained via a silicon intensifier tube camera
mounted on the CSLM. At completion of the imaging procedure,
the kidney was returned into the body cavity, the area was
sutured and the animal was allowed to recuperate for
subsequent examinations. Image processing algorithms, such as
grey-level thresholding, median filtering, skeletonization and
template matching, were applied to compute the vessel density
and diameters and extrapolated to measure 3-D vessel lengths
and the tortuosity index of the neovasculature.



Journal of Microscopy, Vol. 176, Pt. 3, December 1994, pp.
276-280.

Optoelectronic detector probes for scanning near-field optical
microscopy

H. U. Danzebrink
Physikalisch-Technische Bundesanstalt, Bundesallee 100,
D-38116 Braunschweig, Germany


SUMMARY

A brief explanation of the optoelectronic probe concept and a
comparison between the implementation of passive waveguide
probes and optoelectronic probes in scanning near-field
optical microscopy (SNOM) is presented. The first probe
realizations using cleaved semiconductor crystals and the work
at present in progress using microfabricated Si pyramids are
described. These crystals with evaporated metal electrodes
forming a slit aperture with subwavelength dimensions work as
metal-semiconductor-metal photodetectors. Their optical
detection behaviour is investigated by measuring the intensity
distribution of a laser focal point. Measurements where the
external bias voltage is changed show that it is possible to
modify the detection behaviour of the device because of the
varying depletion widths. The last part of the article
describes a concept where pyramidal probes should function
simultaneously as senors for scanning force microscopy (SFM)
to measure topography and as optoelectronic probes for
scanning near-field optoelectronic microscopy (SNOEM).


Journal of Microscopy, Vol. 176, Pt. 3, December 1994, pp.
281-286.

Imaging in the far-red with electronic light microscopy:
requirements and limitations

C. Cullander
School of Pharmacy S926, University of California, San
Francisco, CA 94143-0446, U.S.A.


SUMMARY

The acquisition of simultaneous dual confocal images with red
and far-red light has both advantages (e.g. lower
autofluorescence) and limitations. An understanding of these
requisites is necessary to acquire high-quality images and to
avoid the misinterpretation of experimental data. The poor
detection of far-red light mandates a high optical transfer
efficiency for the system, thus the transmittance of the
objective lens and its axial and lateral chromatic aberration
in the far-red are important factors for consideration. This
technical note is an attempt to 'demystify' the process of
filter set design for confocal microscopy by discussing the
considerations that went into the construction of a filter set
for use with the reagents cyanine 3.18 (Cy3) and cyanine 5.18
(Cy5), and thus to encourage users to look beyond the
multipurpose designs available commercially. The 568-nm laser
line exciting Cy3 is at its emission maximum, which limits the
collectable Cy3 fluorescence. High-transmission optical
filters with sharp bandpass cutoffs are thus desirable for
maximum light throughput. Light path mirror efficiency rapidly
degrades above 700nm, but the loss of this portion of the Cy5
emission spectrum is acceptable since the fluorophore is very
bright, and these very long wavelengths are also likely to
introduce aberration. While resolution is decreased with far-
red light, there is also greater penetration and less
scattering, and it is thus possible to obtain high-quality
images from deeper within the specimen. Although only one make
and model of confocal microscope (the Bio-Rad MRC-600) is
considered, similar considerations pertain to the design of
filter sets for any confocal microscope that accommodates
user-installed filters.



Journal of Microscopy, Vol. 176, Pt. 3, December 1994, pp.
287-299.

Simultaneous confocal recording of multiple fluorescent labels
with improved channel separation

K. Carlsson, N. Aslund, K. Mossberg & J. Philip
Physics IV, The Royal Institute of Tech, S-100 44 Stockholm,
Sweden


SUMMARY

Confocal microscopes are often used to study specimens
labelled with fluorophores. A commonly used method for
simultaneous recording of the distribution of multiple
fluorophores is to divide the fluorescent light emitted by the
specimen into different wavelength regions using dichroic and
bandpass filters. These different wavelength regions are then
distributed to multiple detectors. However, fluorophores often
result in considerable cross-talk between channels. A new
technique, intensity-modulated multiple-beam scanning (IMS)
microfluorimetry, can be used to reduce this cross-talk
substantially.
The IMS technique is implemented with two laser beams of
different wavelengths, intensity-modulated at different
frequencies, which illuminate the specimen simultaneously. The
two laser wavelengths predominantly excite one fluorophore
each. Fluorescent light from the specimen is divided into two
wavelength regions (red and green) which are detected by two
photomultiplier tubes. The output signals from the
photomultiplier tubes are connected to lock-in amplifiers. The
effect of using modulated laser beams, in combination with
lock-in amplifiers, is strongly to reduce the cross-talk
between channels. The performance of the IMS technique using
various types of specimen is compared with the results
obtained using the conventional multi-detector design.




Journal of Microscopy, Vol. 177, Pt. 1, January 1995, pp. 1-6.

In vivo determination of fibril orientation in plant cell
walls with polarization CSLM

J.-P. Verbelen & D. Stickens
Department of Biology, University of Antwerp (UIA),
Universiteitsplein 1, B-2610 Wilrijk-Antwerpen, Belgium


SUMMARY

Congo Red fluorescence is used to detect cellulose in the wall
of plant cells. The orientation of the cellulose fibrils is
determined by using polarized light for excitation. The
absorption characteristics of Congo Red make this approach a
method of choice for applications with any standard confocal
scanning laser microscopy (CSLM). The semi-quantitative
character of CSLM observations combined with the non-toxicity
of the stain allow a very fast and reliable assessment of
cellulose orientation in the wall of living plant cells.



Journal of Microscopy, Vol. 177, Pt. 1, January 1995, pp.
7-17.

Three-dimensional reconstruction of the human renal glomerulus

K. Preston Jr, B. Joe, R. Siderits & J. Welling
Kensal Corporation, 5055 East Broadway (Suite C206), Tucson AZ
85711, U.S.A.


SUMMARY

The capillary bed of human renal glomerulus is one of the more
complex capillary structures in the human body. This paper
illustrates three-dimensional reconstruction of the capillary
bed from serial sections. It shows that, although traditional
methods of three-dimensional rendering by computer fail to
handle the complexities of the capillary structure, new
methods based on filtering using three-dimensional
mathematical morphology are capable of revealing previously
unseen details. This is done at the expense of eliminating
fine structure (small capillaries). An error analysis allows
the degree to which fine details are lost to be estimated.



Journal of Microscopy, Vol. 177, Pt. 1, January 1995, pp.
18-30.

Quantitative water mapping of cryosectioned cells by electron
energy loss spectroscopy

S. Q. Sun, S.-L. Shi, J. A. Hunt & R. D. Leapman
Health & Human Services, Public Health Service, National
Institutes of Health, Building 13 Room 3W13, Bethseda MD
20892, U.S.A.


SUMMARY

A direct technique based on electron energy-loss spectroscopy
(EELS) in the scanning transmission electron microscope (STEM)
has been developed to map subcellular distributions of water
in frozen-hydrated biological cryosections. Previously,
methods for water determination have been indirect, in that
they have required the cryosections to be dehydrated first.
The new approach makes use of spectrum-imaging, where EELS
data are collected in parallel at each pixel. Several
operations are required to process the spectra including:
subtraction of the detector dark current, deconvolution by the
detector point-spread function, removal of plural inelastic
scattering and correction for the support film. The resulting
single scattering distributions are fitted to standard
reference spectra at each pixel, and water content can be
determined from the fitting coefficients. Although the
darkfield or brightfield image from a hydrated cryosection
shows minimal structure, the processed EELS image reveals
strong contrast due to variation in water content. Reference
spectra have been recorded from the major biomolecules
(Protein, lipid, carbohydrate, nucleic acid) as well as from
vitrified water and crystalline ice. It has been found that
quantitative results can be obtained for the majority of
subcellular compartments by fitting only water and protein
reference spectra, and the accuracy of the method for these
compartments has been estimated as plus/minus 3.5%. With the
present instrumentation the maximum allowed dose of 2000e/nm2
limits the useful spatial resolution to around 80nm plus/minus
5% precision at a single pixel. By averaging pixel intensities
a value of 56.8% with a precision of plus/minus 2.0% has been
determined for the water content of liver mitochondria. The
water mapping technique may prove useful for applications to
cell physiology and pathophysiology.



Journal of Microscopy, Vol. 177, Pt. 1, January 1995, pp.
31-42.

Hybrid scanning transmission electron/scanning tunnelling
microscope system for the preparation and investigation of
biomolecules

H. F. Knapp, R. Wyss, R. Haring, C. Henn, R. Guckenberger & A.
Engel
Maurice E Muller Institut fur, Hochauflosende
Elektronenmikroskopie, Universitat Basel, Klingelbergstrasse
70, CH-4056 Basel, Switzerland


SUMMARY

A hybrid scanning transmission electron/scanning tunnelling
microscope vacuum system is introduced, which allows freeze
drying and metal coating of biological samples and their
simultaneous observation by scanning transmission electron
microscopy and scanning tunnelling microscopy (STM). Different
metal coatings and STM tips were analysed to obtain the
highest possible resolution for such a system. Bovine liver
catalase was used as a test sample and the STM results are
compared to a molecular scale model.


Journal of Microscopy, Vol. 177, Pt. 1, January 1995, pp.
43-52.

Backscattered electron imaging of the undersurface of
resin-embedded cells by field emission scanning electron
microscopy

R. G. Richards & I. Ap Gwynn
AO/ASIF Research Institute, Clavadelerstrasse, CH-7270 Davos
Platz, Switzerland


SUMMARY

In this study backscattered electron (BSE) imaging was used to
display cellular structures stained with heavy metals within
an unstained resin by atomic number contrast in successively
deeper layers. Balb/c3T3 fibroblasts were cultured on either
13mm discs of plastic Thermanox, commercially pure titanium or
steel. The cells were fixed, stained and embedded in resin and
the disc removed. The resin block containing the cells was
sputter coated and examined in a field-emission scanning
electron microscope. The technique allowed for the direct
visualization of the cell undersurface and immediately
overlying areas of cytoplasm through the surrounding embedding
resin, with good resolution and contrast to a significant
depth of about 2 micrometre, without the requirement for
cutting sections. The fixation protocol was optimized in order
to increase heavy metal staining for maximal backscattered
electron production. The operation of the microscope was
optimized to maximize the number of backscattered electrons
produced and to minimize the spot size. BSE images were
collected over a wide range of accelerating voltages (keV),
from low values to high values, to give 'sections' of
information from increasing depths within the sample. At 3-
4keV only structures a very short distance into the material
were observed, essentially areas of cell attachment to the
removed substrate. At higher accelerating voltages information
on cell morphology, including in particular stress fibres and
cell nuclei, where heavy metal were intensely bound, became
more evident. The technique allowed stepwise 'sectional'
information to be acquired. The technique should be useful for
studies on cell morphology, cycle and adhesion with greater
resolution than can be obtained with any light-microscope-
based system.



Journal of Microscopy, Vol. 177, Pt. 1, January 1995, pp.
53-67.

Tomographic reconstruction of the cross-sectional refractive
index distribution in semi-transparent birefringent fibres

T. C. Wedberg & W. C. Wedberg
Physics Department, University of Bergen, Allegt 55, N-5007
Bergen, Norway


SUMMARY

Optical diffraction tomography (ODT) is used to reconstruct
the complex refractive index distribution in cross-sections of
semi-transparent, birefringent fibres. The selected fibres
were polymer and animal fibres of either circular or non-
circular cross-section with average thicknesses in the range
8-110 micrometre. This choice of samples was made to
illustrate the imaging capabilities of ODT, and also to
demonstrate some potential applications of the technique. The
images representing the reconstructed refractive index
distributions have a spatial resolution of about 2 micrometre,
and show noticeable image contrast for refractive index
variations of about 0.001. The ODT reconstructions compare
well with refractive index information provided with the
samples, and with scanning electron micrographs of cross-
sections of the same fibre samples. From these results it
appears that ODT can be used to reconstruct the complex
refractive index distribution in cross-sections of semi-
transparent birefringent fibres.



Journal of Microscopy, Vol. 177, Pt. 1, January 1995, pp.
68-76.

Computer simulated high-resolution transmission electron
microscopy (HRTEM) in tourmaline

E. A. Ferrow
Avdelningen for Mineralogi och Petrologi, Geologiska
Institutionen, Lund Universitet, Solvegatan 13, 223 62 Lund,
Sweden


SUMMARY

The contrast distributions observed in high-resolution
transmission electron microscopy (HRTEM) images of tourmaline
depend on the types and magnitudes of the exchange components
present and on the degree of atom overlap along the direction
of observation. Furthermore, the fractional atomic coordinates
in tourmalines are valid only for the specific specimen
refined. These properties make the interpretation of
experimental HRTEM images of tourmaline using image simulation
if not impossible at least extremely difficult. A correct
interpretation of experimental HRTEM images of tourmaline is
possible provided the structural refinement data on the same
crystal are available. Nevertheless, it is possible to
interpret the experimental HRTEM images of tourmaline if the
composition of the structural model chosen during image
simulation approximates the composition of the specimen
studied by electron microscopy. A good control of the
composition of the specimen studied and an appropriate choice
of a structural model for image simulation are therefore as
important as properly controlling specimen thickness, specimen
tilt, beam tilt and objective lens defocus.



Journal of Microscopy, Vol. 177, Pt. 1, January 1995, pp.
77-84.

Confocal microscopy in the analysis of the etched nuclear
particle tracks in polymers

J. Jakes, P. Gais & H. Schraube
Institut fur Strahlenschutz, GSF-Neuherberg, Postfach 1129,
D-85758 Oberschleissheim, Germany


SUMMARY

The possibility of the morphometric analysis of etched tracks,
induced by protons and alpha particles in the organic polymer
allyl diglycol carbonate (CR-39), using the confocal scanning
laser microscope (CSLM), was studied. The detectors were
investigated in two groups of irradiation experiments, namely:
(a) irradiated with mono-energetic neutrons of energy 1.2MeV,
(b) exposed to the alpha radiation from 222Rn and its progeny.
Both groups were irradiated at normal incidence. Radiation-
induced latent tracks were electrochemically etched, and their
morphometric parameters were investigated in the reflection
mode by using the 488nm spectral line of an argon ion laser. A
constant number of up to 200 optical sections in Z-scan mode
was taken through each selected etched track at vertical
spacings of 0.642 micrometre. Successive reconstructions of Z-
sections were used to determine the following parameters: the
mean radius of the opening channel, the maximum diameter and
the length of the track, and the angle of the track wall to
the surface of the sample. The results show that tracks
produced by alpha particles differ from those induced by
protons. The radius of the opening channel of alpha-particle-
induced tracks ranges from 7.9 to 11 micrometre, whereas for
protons the same parameter ranges between 2.0 and 3.8
micrometre for a specific electrochemical etch procedure.



Journal of Microscopy, Vol. 177, Pt. 1, January 1995, pp.
85-89.

A simple method for overcoming some problems when observing
thick reflected biological samples with the confocal scanning
laser microscope

C. Rumio, M. Morini, J. R. Miani, I. Barajon & P. Castano
Institute of Human Anatomy, Via Mangiagalli 31, 20133 Milano,
Italy


SUMMARY

A simple device is described, which allows the range of depth
of scanning to be reduced when observing thick reflecting
biological specimens with a confocal scanning laser microscope
(CSLM). Thick histological sections of human skin and rat
brain stem were mounted between two coverslip ('sandwich
style') and the optical tomography was performed from both
sides by turning the 'sandwich' upside-down. The samples were
impregnated using standard Golgi-Cox, 'rapid Golgi' or other
silver methods. The ability to turn the sandwich upside-down
is particularly useful when the reflective structure inspected
is deep inside the section, i.e. near the lower surface of the
specimen, or when it is opaque to the laser beam of
excessively reflective.


Journal of Microscopy, Vol. 177, Pt. 1, January 1995, pp.
90-92.

Thick section preparation using a silicon-rubber-based sealant

H. Cox, C. Walker & C. V. Howard
Department of Orthopaedic & Accident Surgery, Royal Liverpool
University Hospital, Prescot Street, PO Box 147, Liverpool,
L69 3BX


SUMMARY

A method has been developed, using a silicon-rubber-based
sealant, which allows 2-3-mm-thick specimens to be maintained
in a protected fluid environment for a number of months,
without risk of dehydration. Following this, the specimen can
be retrieved, stained, embedded and sectioned further. For
example, 2-mm-thick sections of fixed unstained bone are
easily examined by means of epi-illuminated polarized light
and fluorescence microscopies using either conventional or
confocal optics. The method could easily be extended to other
tissues, for example brain tissue.



Journal of Microscopy, Vol. 177, Pt. 2, February 1995, pp. .

A method to compensate for light attenuation with depth in
three-dimensional DNA image cytometry using a confocal
scanning laser microscope

A. Liljeborg, M. Czader & A. Porwit
Physics IV, The Royal Institute of Tech, S-100 44 Stockholm,
Sweden


SUMMARY

A method to compensate for attenuation of detected light with
increased depth of the collected optical section, and its
application in three-dimensional (3-D) DNA image cytometry is
described. The method is based on studying the stack of 2-D
histograms that ca be formed from each consecutive pair of
sections in a stack of optical serial sections. An attenuation
factor is calculated interactively and a new compensated
section series is computed. Formalin-fixed paraffin-embedded
rat tissue was stained with propidium iodide. Each cell
nucleus is extracted by thresholding and its total intensity
is calculated. The coefficient of variation (CV) of the total
intensity of all cells in each stack is computed. For
comparison the CV of the same cells is computed in the
uncompensated stacks. This study shows a significantly lower
CV for the compensated data, thus contributing to the accuracy
of DNA quantification in 3-D DNA image cytometry.


Journal of Microscopy, Vol. 177, Pt. 2, February 1995, pp. .

Microfractography of granitic rocks under confocal scanning
laser microscopy

M. Montoto, A. Martinez-Nistal, A. Rodriguez-Rey, N.
Fernandez-Merayo & P. Soriano
University of Oviedo, Department of Geology, Group of
Petrophysics, 33005 Oviedo, Spain


SUMMARY

Scanning laser microscopy, in the confocal mode (CSLM) has
been applied to a granitic rock to characterize its fissure
space. The technique provides a unique three-dimensional
picture of the rock microfractomography. CSLM is unique in
observing fine details of the fractographic network
(connectivity, tortuosity, etc.), its geometry and its
relation to other rock-forming components.
The fractographic images with standard fluorescence
microscopy are compared with those obtained with CSLM. The
examples presented emphasize the advantages of CSLM: three-
dimensional visualization of the microfractographic network,
crack connectivity, automatic evaluation of direction and
slope of fissures.
These studies are related to the migration of
radionuclides in the geosphere. The relations between
potentially water-conducting open fissures and the rock-
forming minerals provide a means of modelling the
'radionuclide retardation mechanism', a security factor in
their definitive storage in rock masses.


Journal of Microscopy, Vol. 177, Pt. 2, February 1995, pp. .

PHOEBE, a prototype scanning laser-feedback microscope for
imaging biological cells in aqueous media

T. L. Wong, S. L. Sabato & A. Bearden
Division of Neurobiology, Department of Molecular & Cell
Biology, 229 Stanley Hall, University of California at
Berkeley, Berkeley CA 94720-3206, U.S.A.


SUMMARY

Based on the principle of laser-feedback interferometry (LFI),
a laser-feedback microscope (LFM) has been constructed,
capable of providing an axial (z) resolution of a target
surface topography of approximately 1nm and a lateral (x,y)
resolution of approximately 200nm when used with a high-
numerical-aperture oil-immersion microscope objective. LFI is
a form of interferometry in which a laser's intensity is
modulated by light re-entering the illuminating laser.
Interfering with the light circulating in the laser resonant
cavity, this back-reflected light gives information about an
object's position and reflectivity. Using a 1-mW He-Ne
(wavelength=632.8nm) laser, this microscope (PHOEBE) is
capable is obtaining 256x256-pixel images over fields from
10x10 micrometre to 120x120 micrometre in approximately 30s.
An electrochemical feedback circuit holds the optical
pathlength between the laser output mirror and a point on the
scanned object constant; this allows two types of images
(surface topography and surface reflectivity) to be obtained
simultaneously. For biological cells, imaging can be
accomplished using back-reflected light originating from small
refractive-index changes (} 0.02) at cell membrane/water
interfaces; alternatively, the optical pathlength through the
cell interior can be measured point-by-point by growing or
placing a cell suspension on a higher-reflecting substrate
(glass or silicon wafer). Advantages of the laser-feedback
microscope in comparison to other confocal optical microscopes
include: simplicity of the single-axis interferometric design;
the confocal property of the laser-feedback microscope (a
virtual pinhole), which is achieved by the requirement that
only light that re-enters the laser meeting the stringent
frequency, spatial (TEM00), and coherence requirements of the
laser cavity resonator mode modulate the laser frequency; and
the improved axial resolution, which is based on
interferometric measurement of optical amplitude and phase
rather than by use of a pinhole as in other types of confocal
microscopes.



Journal of Microscopy, Vol. 177, Pt. 2, February 1995, pp. .

Imaging periodic surface relief structures

J. T. Sheridan & T. O. Korner
TP 680, Institute for System Engineering and, Informatics
Science R&D, Joint Research Centre JRS/CCR, I-21020 Ispra
(VA), Italy


SUMMARY

Because of shadowing, multiple scatter and polarization
effects, the interpretation of images of grating with fine
periods, isolated deep structures, and multiple scattering
volume objects is seriously complicated. In this paper a
review of methods used to model such effects is presented.
Periodic surface relief gratings are of particular current
importance because of the possibility of producing calibration
samples using them. Several examples which illustrate
electromagnetic volume effects are examined. General trends
which help in validating the use of Fourier-transform-based
scalar transmittance theory are then indicated. The angular
spectrum approach, which can be used , together with a scatter
function generated using the rigorous electromagnetic theory,
to calculate coherent, partially coherent and confocal images
of volume objects, is also discussed.


Journal of Microscopy, Vol. 177, Pt. 2, February 1995, pp. .

Application of confocal laser microscopy and three-dimensional
Voronoi diagrams for volume and surface area estimates of
interphase chromosomes

R. Eils, E. Bertin, K. Saracoglu, B. Rinke, E. Schrock, Y.
Usson, M. Robert-Nicoud, E. H. K. Stelzer, J.-M. Chassery, T.
Cremer & C. Cremer
Institut fur Angewandte Physik, Albert-Uberle Strasse 3-5,
D-69120 Heidelberg, Germany


SUMMARY

This study demonstrates the use of Voronoi tessellation
procedures to obtain quantitative morphological data for
chromosome territories in the cell nucleus. As a model system,
chromosomes 7 and X were visualized in human female amniotic
fluid cell nuclei by chromosomal in situ suppression
hybridization with chromosome-specific composite probes. Light
optical serial sections of 18 nuclei were obtained with a
confocal scanning laser fluorescence microscope. A three-
dimensional (3-D) tessellation of the image volumes defined by
the stack of serial sections was then performed. For this
purpose a Voronoi diagram, which consists of convex polyhedra
structured in a graph environment, was built for each nucleus.
The chromosome territories were then described by three
morphological parameters, i.e. volume, surface area and a
roundness factor (shape factor). The complete evaluation of a
nucleus, including the calculation of the Voronoi diagram, 3-D
visualization of extracted territories using computer graphic
methods and parameterization was carried out on a Silicon
Graphics workstation and was generally completed within 5 min.
The geometric information obtained by this procedure revealed
that both X- and 7-chromosome territories were similar in
volume. Roundness factors indicated a pronounced variability
in interphase shape for both pairs of chromosomes. Surface
estimates showed a significant difference between the two X-
territories but not between chromosome 7-territories.


Journal of Microscopy, Vol. 177, Pt. 2, February 1995, pp. .

Forbidden light scanning near-field optical microscopy

H. Heinzelmann, B. Hecht, L. Novotny & D. W. Pohl
Institut fur Physik, Universitat Basel, Klingelbergstrasse 82,
4056 Basel, Switzerland


SUMMARY

Near-field optics (NFO) opens the door to light microscopy
techniques with resolutions well beyond the diffraction limit.
The richness of optical investigations is now applicable on a
near-molecular level. Among the novel scanning near-field
optical microscopy (SNOM) schemes, the most prominent
representatives are aperture SNOM and scanning tunnelling
optical microscopy (STOM or PSTM).
New experimental and theoretical work has to be performed
to study the phenomena specific to NFO. One such example is
the angular dependence of light emission in aperture SNOM. The
detection of radiation at angles greater than the critical
angle of total internal reflection alpha=arcsin(1/n), where n
is the sample refractive index, can represent a microscopy
scheme that combines the respective advantages of both
aperture SNOM and STOM. Recent experiments have demonstrated
the expected exponential dependence of light intensity on gap
width (for fixed emission angle). The decay length as a
function of alpha is in agreement with the Fresnel description
of the evanescent field when total reflection occurs at an
interface. These investigations were additionally motivated by
calculations based on the multiple multipole method.



Journal of Microscopy, Vol. 177, Pt. 2, February 1995, pp. .

Automated correction of linear deformation due to sectioning
in serial micrographs

T. Jansson, T. Gustavsson, M. Rydmark, C.-H. Berthold, R.
Pascher & T. Skoglund
Department of Applied Electronics, Chalmers University of
Technology, S-412 96 Goteborg, Sweden


SUMMARY

This paper describes an objective and automatic method for
detection and correction of sectioning deformations in
digitized micrographs, as well as an evaluation of the method
applied to light and electron microscopic images of semi-thin
and ultra-thin serial sections from brain cortex. The
detection is based on matching of image subregions and the
deformation model is bi-linear, i.e. two first-order
polynomials are used for modelling compression/expansion in
perpendicular directions. The procedure is applicable to
prealigned serial two-dimensional sections and is primarily
aimed at three-dimensional reconstruction of tissue samples
consisting of a large number of cells with random distribution
and morphology.


Journal of Microscopy, Vol. 177, Pt. 2, February 1995, pp. .

Comparison of three-dimensional imaging properties between
two-photon and single-photon confocal fluorescence microscopy

Min Gu & C. J. R. Sheppard
Physical Optics Department, School of Physics, The University
of Sydney, NSW 2006, Australia


SUMMARY

The imaging performance in single photon (1-p) and two-photon
(2-p) fluorescence microscopy is described. Both confocal and
conventional systems are compared in terms of the three-
dimensional (3-D) point spread function and the 3-D optical
transfer function. Images of fluorescent sharp edges and
layers are modelled, giving resolution in transverse and axial
directions. A comparison of the imaging properties is also
given for a 4Pi confocal system. Confocal 2-p 4Pi fluorescence
microscopy gives the best axial resolution in the sense that
its 3-D optical transfer function has the strongest response
along the axial direction.


Journal of Microscopy, Vol. 177, Pt. 2, February 1995, pp. .

Double pulse fluorescence lifetime imaging in confocal
microscopy

M. Muller, R. I. Ghauharali, K. Visscher, T. D. Visser & G. J.
Brakenhoff
Department of Molecular Cytology, University of Amsterdam,
Plantage Muidergracht 14, 1018 TV Amsterdam, The Netherlands


SUMMARY

A theoretical analysis of a new technique for fluorescence
lifetime measurement, relying on (near steady state)
excitation with short optical pulses, is presented.
Application of the technique to confocal microscopy enables
local determination of the fluorescence lifetime, which is a
parameter sensitive to the local environment of fluorescent
probe molecules in biological samples. The novel technique
provides good time resolution, since it relies on the laser
pulse duration, rather than on electronic gating techniques,
and permits, in combination with bilateral confocal microscopy
and the use of a (cooled) CCD, sensitive signal detection over
a large dynamic range. The principle of the technique is
discussed within a theoretical framework. The sensitivity of
the technique is analysed, taking into account:
photodegradation, the effect of the laser repetition rate and
the effect of non-steady-state excitation. The features of the
technique are compared to more conventional methods for
fluorescence lifetime imaging.


Journal of Microscopy, Vol. 177, Pt. 2, February 1995, pp. .

Near field imaging: some attempts to define an apparatus
function

D. Courjon
Laboratoire d'Optique PM Duffieux, Associe au CNRS URA-214,
UFR des Sciences et des Techniques, 25030 Besancon Cedex,
France


SUMMARY

Near-field microscopy is a promising new tool capable of
imaging details smaller than the wavelength. The mechanism of
imaging is analysed and an overview of the apparatus functions
which could be used to define an image quality criterion is
given.


Apologies for sending such a long file at once. Please note that summaries will
be posted less frequently as Gillian Wilson is now on maternity leave.

************************************************************************

Gillian Wilson Executive Editor
The Royal Microscopical Society Journal of Microscopy &
37/38 St Clements Proceedings of the RMS
Oxford
OX4 1AJ UNITED KINGDOM
rms-at-uk.ac.ox.vax OR rms-at-vax.ox.ac.uk
Telephone +44 (0)865 248768
Fax +44 (0)865 791237

************************************************************************




From: imgp-at-mbimp1.mbl.TNO.NL (Kees van der Wulp)
Date: Mon, 30 Jan 1995 12:39:54 +0100 (MET)
Subject: addition to : Re. 2D softw. for Unix

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear microscopists,

I apologize for not being complete regarding the URL I gave
on the SCIL-Image software. Here is a more precise specification
of the path towards the information.

URL: http://www.tn.tudelft.nl

select : Pattern Recognition Group -
Software Developments -
SCIL-Image

Hope this saves you time.

--
Kees van der Wulp

******************************* ATTENTION ***************************
* *
* Change in INTERNET address ! *
* *
* From : vanderwulp-at-mbl.tno.nl *
* To : vanderwulp-at-voeding.tno.nl *
* *
*********************************************************************

TNO - Nutrition & Food Research INTERNET : vanderwulp-at-voeding.tno.nl
Division : Toxicology VOICE : +31 15 843101
Department : Genetic Toxicology FAX : +31 15 843989
PO-Box 5815 General TNO Info : http://www.tno.nl
2280 HV RIJSWIJK (NL)
THE NETHERLANDS




Groeten,
Gert van Antwerpen.
TNO Institute of Applied Physics.
P.O.Box 155, 2600 AD Delft, The Netherlands.
Phone: +31 15-692226, Fax: +31 15-692111
Email: antwerp-at-tpd.tno.nl

--
Kees van der Wulp

******************************* ATTENTION ***************************
* *
* Change in INTERNET address ! *
* *
* From : vanderwulp-at-mbl.tno.nl *
* To : vanderwulp-at-voeding.tno.nl *
* *
*********************************************************************

TNO - Nutrition & Food Research INTERNET : vanderwulp-at-voeding.tno.nl
Division : Toxicology VOICE : +31 15 843101
Department : Genetic Toxicology FAX : +31 15 843989
PO-Box 5815 General TNO Info : http://www.tno.nl
2280 HV RIJSWIJK (NL)
THE NETHERLANDS





From: Glen Macdonald :      glenmac-at-u.washington.edu
Date: Mon, 30 Jan 1995 10:33:16 -0800 (PST)
Subject: Here's Dabco references

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-Sender: glenmac-at-homer07.u.washington.edu

The references:

1,4-Diazobicyclo(2,2,2)octane (Dabco) delays fading of
immunofluorescence preparations
Langanger, Gabriele; De Mey, Jan; Adam, Hans
Mikroskopie 1983; 40 (7-8): 237-41
Language: German

Fading of immunofluorescence during microscopy. A study of the
phenomenon and its remedy
Johnson G D; Davidson R S; Mcnamee K C; Russell G; Goodwin D;
Holborow E J
J Immunol Methods 55 (2). 1982. 231-242

Regards,
Glen MacDonald
Hearing Development Laboratories RL-30
University of Washington
Seattle, WA 98195
(206)543-8360
glenmac-at-u.washington.edu






From: sbarlow-at-sunstroke.sdsu.edu (Steve Barlow)
Date: Mon, 30 Jan 1995 10:46:55 -0800
Subject: Re EM embedding

Contents Retrieved from Microscopy Listserver Archives
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Jon

I encountered the identical problem of dark caramel colored epon mix after
addition of DMP-30, Substitution of the standard DDSA component with a
new bottle of "Specially Distilled DDSA" corrected the problem and
produced the golden colored resin mix. I was also told (see Marge Hukee's
reply) the problem was a function of the pH of the resins

----------------------------------------------------------
Dr. Steven Barlow
EM Facility/Biology Dept.
San Diego State University
San Diego CA 92182-0057
phone: (619) 594-4523
fax: (619) 594-5676
email to sbarlow-at-sunstroke.sdsu.edu





From: imgp-at-mbimp1.mbl.TNO.NL (Kees van der Wulp)
Date: Tue, 31 Jan 1995 11:09:54 +0100 (MET)
Subject: Re: 2D IP software for Unix

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Microscopy friends,

Because I still miss the original message and only received the additional
message I posted (Re : 2D software for Unix), I repost the original correc-
ted message to the microscopy list.
I apologize for this 2nd message if you DID receive the first one somehow.

Dear Norm,

Regarding your question,

}
} I ask for comments, recommendations, advice, for 2D image analysis software
} for a UNIX platform (SGI Indy R4000).
}
} If there is interest I'll post a summary. Thanks.
}

I can give you 2 URL's, leading to the same SCIL page, that provide you with
a brief description of SCIL-Image (version 1.3), as well as an e-mail address
of Mr. G. van Antwerpen whom I gave your address. He will take care of providing
you with more information on SCIL-Image.

I am using SCIL-Image on my Personal IRIS 4D/35 for a couple of years now.
It is an extensive 2D multi-layered image processing package (} 200 ip commands).
It can be used on various platforms (Sun, HP, SGI, IBM, PC's, Mac's)
and can be run from a mouse driven menu(1), a commandline interpreter (2)
and from compiled routines (3) which makes it run very fast.
It is a combination of a Standard C Interpreter Language (part 1) and
an extensive Image processing library (part 2). It incorporates a complete program
flow control mechanism and enables you to create and mix C- statements
(and complete programs) with image processing commands. You can also easily extend
the package with your own library of user created (compiled or C-source)
routines and incorporate them into the mouse driven menu.
I run it from my X-Terminal (Tektronix XP 27 and XP 356, colour terminals)
in interactive as well as batch mode.
I heard from mr. van Antwerpen that you can evaluate the full package for
a period of 3 months at a rate of about US$ 250.= However for these kind
of things please contact him, for I am only a user of the package.

URL: http://www.tn.tudelft.nl

select : Pattern Recognition Group -
Software Developments -
Scil-Image

or
URL: http://galaxy.ph.tn.tudelft.nl:2000/Software/scil.html


e-mail address G. van Antwerpen : antwerp-at-tpd.tno.nl

All people interested in more info can get it from him.

Good luck,

--
Kees van der Wulp

TNO - Nutrition & Food Research INTERNET : vanderwulp-at-voeding.tno.nl
Division : Toxicology VOICE : +31 15 843101
Department : Genetic Toxicology FAX : +31 15 843989
PO-Box 5815 General TNO Info : http://www.tno.nl
2280 HV RIJSWIJK (NL)
THE NETHERLANDS





From: slc6-at-Lehigh.EDU (SHARON L. COE)
Date: Tue, 31 Jan 1995 08:58:26 EST
Subject: Microscopy courses at Lehigh

Contents Retrieved from Microscopy Listserver Archives
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Message-Id: {199501311358.IAA08013-at-ns1.CC.Lehigh.EDU}

1995 MICROSCOPY SHORT COURSES AT LEHIGH UNIVERSITY
25TH ANNIVERSARY
June 12-23, 1995

Scanning Electron Microscopy and X-ray Microanalysis (June 12-16, 1995)

Advanced Scanning Electron Microscopy with Digital Imaging (June 19-22, 1995)

Quantitative X-ray Microanalysis of Bulk Specimens and Particles (June 19-22,
1995)

Atomic Force Microscopy and other Scannned Probe Microscopies (June 20-23, 1995)

Analytical Electron Microscopy (June 19-22, 1995)

For more information please e-mail a response with subject "Short Course Info"
to Sharon Coe at slc6-at-lehigh.edu or call 610/758-5133.

If you e-mail, please leave name and postal address and I will send you a
brochure and registration form.






From: ischmitz-at-fbch.tuwien.ac.at (Ingo SCHMITZ)
Date: Tue, 31 Jan 1995 16:20:30 +0100 (MET)
Subject: NEW software for LINK users

Contents Retrieved from Microscopy Listserver Archives
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To all LINK / JEOL users,

We have developed a new method for fast data transfer from
a JEOL microscope connected to a LINK eXL computer system
to DOS based personal computers.

In our institute we are using a JEOL JSM 6400 SEM
equipped with an energy dispersive detector driven by
a LINK eXL computer running the GENIE operating system.

In former days we experienced great problems transferring
images captured from the screen and stored in tiff format
to DOS based computers, since we neither posses any GENIE
image processing software nor do we have network
connectivity on the Link system.

That's why we have developed a unique hardware/software
solution which allows users to transfer tiff files at the
rate of approx. 1 sec. per file (262 KB) from the Link
computer to the DOS computer.

The software is capable of viewing the contents of GENIE
formatted floppy disks, hard disks, MO's and changeable
devices (e.g. Syquest drives) and copying selected images
and spectra to the target computer.

Anyone interested may contact us for further details at:


email: ischmitz-at-fbch.tuwien.ac.at
snikolov-at-email.tuwien.ac.at

phone: ++43-1-58801-4852
-4843

FAX: ++43-1-5867813

mail: Ingo Schmitz
University of Technology
Institute of Analytical Chemistry E151
Getreidemarkt 9/151
A-1060 Vienna
Austria







From: JoRita Jordan :      jjordan-at-world.std.com
Date: Tue, 31 Jan 1995 15:25:22 +0001 (EST)
Subject: TEM survey

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


TEM Users:

I publish a monthly newsletter, Analytical Consumer, that reports on
customer satisfaction with manufacturers of analytical equipment. We are
currently doing a survey of TEM users, similar to an SEM study we published
last July, in conjunction with Microscope News & Technology (who is also
collaborating on this study). I am looking for labs with TEMs who would be
willing to talk about their equipment.

I'm looking for:

What kind of TEM (manufacturer and model) do you use?
How old is it (roughly)?
Why did you buy from that particular manufacturer?
Have you been satisfied with the equipment?
Are you satisfied with the service? Do you get service from the equipment
mfr, do it yourself, or have an outside service supplier? Do you have a
service contract?
What kind of samples do you typically use the TEM for?
What kind of lab are you in? R&D, research, instrument facility, service
lab, or ...? What kind of company is the lab?

If you answer these questions and want a copy of the survey, please include
your address. If you would like to talk about it in person, give me a call
at (508) 369-9079, or send me your phone number and I'll call you.

Thanks!

Jo Rita Jordan
Editor and Publisher
Analytical Consumer

jjordan-at-world.std.com
or
76150.2171-at-compuserve.com




From: richard.easingwood-at-stonebow.otago.ac.nz (Richard Easingwood)
Date: Wed, 1 Feb 1995 11:09:03 +1300
Subject: NZ Microscopy Conference

Contents Retrieved from Microscopy Listserver Archives
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MICROSCOPY CONFERENCE
(In conjunction with the 18th National Conference
of the New Zealand Society for Electron Microscopy)

Dunedin, New Zealand
4th - 8th September 1995.


A Microscopy Conference will be held in Dunedin, 4th to 8th September,
1995. The conference will be held in conjunction with the 18th National
Conference for the New Zealand Society for Electron Microscopy (NZSEM)
however will cover all aspects of Microscopy with emphasis on the
techniques of both Light Microscopy and Electron Microscopy.

The venue for the conference will be the Otago Medical School.

Workshop sessions will run on Monday and Tuesday (Sept 4th - 5th); the
conference proper will run from Wednesday until midday Friday (Sept 6th -
8th).

Guest speakers include:

Professor John M Robinson, Ohio State University, U.S.A.
Professor Robinson has published widely on the practical aspects of enzyme
cytochemistry (in particular the recently developed cerium-based
techniques) and immunocytochemistry. His application of these techniques
utilises many forms of microscopy including conventional light microscopy,
confocal microscopy, transmission electron microscopy and scanning electron
microscopy.

Professor Anthony S-Y Leong, University of Adelaide, Australia.
Professor Leong is well known for his work with microwave techniques, both
at the light microscope and the electron microscope level. His application
of microwave technology includes fixation and processing for L.M. and
T.E.M., and the use of microwaves in immunocytochemistry.

Dr Brian Brooker, Institue of Food Research, England.
Dr Brooker is a food scientist particularly interested in the structure and
behaviour of oil-in-water emulsions and the influence of emulsifiers on
their functions. He applies many microscopy techniques to study these
difficult samples including conventional light microscopy, confocal
microscopy, freeze fracture, X-ray microanalysis, cryofixation and electron
microscopy.

Dr Brendon Griffin, Centre for Microscopy and Microanalysis, Perth, Australia.
Dr Griffin's field of interest are the microscopy of rare minerals. He is
experienced in microprobe analysis particularly EDS, environmental SEM and
field emission SEM. He has a particular interest in EM education. Dr
Griffin is currently president of the Australian Society for Electron
Microscopy.

Trades displays will be a feature of the Conference. We are also planning
a techniques forum with the invited guests forming the panel.

If you are interested in receiving more information about this conference
you are invited to contact the organising committee at the address below;

Allan Mitchell
Organising Committee, 1995 Microscopy Conference
C/- Department of Anatomy and Structural Biology
Otago Medical School
P.O. Box 913 Tel; National 03 479 7301 International 64 3 479 7301
Dunedin Fax; National 03 479 7254 International 64 3 479
7254
New Zealand. email address; allan.mitchell-at-stonebow.otago.ac.nz




Richard Easingwood
Department of Anatomy and Structural Biology,
P.O. Box 913
University of Otago,
Dunedin, New Zealand.
Fax:64-3-479 7254
Telephone:64-3-479 7301






From: M.Dickson-at-unsw.edu.au (Mel Dickson)
Date: Wed, 1 Feb 1995 10:56:20 +1100
Subject: TEM survey

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TEM Users:

I publish a monthly newsletter, Analytical Consumer, that reports on
customer satisfaction with manufacturers of analytical equipment. We are
currently doing a survey of TEM users, similar to an SEM study we published
last July, in conjunction with Microscope News & Technology (who is also
collaborating on this study). I am looking for labs with TEMs who would be
willing to talk about their equipment.

I'm looking for:

What kind of TEM (manufacturer and model) do you use?
How old is it (roughly)?
Why did you buy from that particular manufacturer?
Have you been satisfied with the equipment?
Are you satisfied with the service? Do you get service from the equipment
mfr, do it yourself, or have an outside service supplier? Do you have a
service contract?
What kind of samples do you typically use the TEM for?
What kind of lab are you in? R&D, research, instrument facility, service
lab, or ...? What kind of company is the lab?

If you answer these questions and want a copy of the survey, please include
your address. If you would like to talk about it in person, give me a call
at (508) 369-9079, or send me your phone number and I'll call you.

Thanks!

Jo Rita Jordan
Editor and Publisher
Analytical Consumer

jjordan-at-world.std.com
or
76150.2171-at-compuserve.com






From: M.Dickson-at-unsw.edu.au (Mel Dickson)
Date: Wed, 1 Feb 1995 10:56:19 +1100
Subject: TEM survey

Contents Retrieved from Microscopy Listserver Archives
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TEM Users:

I publish a monthly newsletter, Analytical Consumer, that reports on
customer satisfaction with manufacturers of analytical equipment. We are
currently doing a survey of TEM users, similar to an SEM study we published
last July, in conjunction with Microscope News & Technology (who is also
collaborating on this study). I am looking for labs with TEMs who would be
willing to talk about their equipment.

I'm looking for:

What kind of TEM (manufacturer and model) do you use?
How old is it (roughly)?
Why did you buy from that particular manufacturer?
Have you been satisfied with the equipment?
Are you satisfied with the service? Do you get service from the equipment
mfr, do it yourself, or have an outside service supplier? Do you have a
service contract?
What kind of samples do you typically use the TEM for?
What kind of lab are you in? R&D, research, instrument facility, service
lab, or ...? What kind of company is the lab?

If you answer these questions and want a copy of the survey, please include
your address. If you would like to talk about it in person, give me a call
at (508) 369-9079, or send me your phone number and I'll call you.

Thanks!

Jo Rita Jordan
Editor and Publisher
Analytical Consumer

jjordan-at-world.std.com
or
76150.2171-at-compuserve.com






From: Riitta Harjula :      rharjula-at-cc.oulu.fi
Date: Wed, 1 Feb 1995 14:38:54 +0200 (EET)
Subject: TEM survey

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Subscribe Riitta Harjula

Riitta.Harjula-at-oulu.fi




From: Marc Brande :      brande-at-sdsc.edu
Date: Wed, 1 Feb 1995 11:39:27 -0800 (PST)
Subject: Confocal Scope in San Diego

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Microscopy List {microscopy-at-aaem.amc.anl.gov} ,
Confocal Microscopy List {confocal-at-ubvm.bitnet}
Message-Id: {Pine.3.05.1.9502011127.A7624-9100000-at-pauline.sdsc.edu}
Mime-Version: 1.0
Content-Type: TEXT/PLAIN; charset=US-ASCII

I am in need of access to a confocal scope in the San Diego area. Would
anyone know of a lab that has one and who to contact? Thanks in advance
for your help.

Marc

Marc C. Brande, M.S. SD3D Email List:3D Imaging
San Diego 3D Imaging Group To subscribe/unsubscribe:listserv-at-bobcat.etsu.edu
3840 Camino Lindo To post a message:sd3d-at-bobcat.etsu.edu
San Diego, CA 92122 Email:BRANDE-at-SDSC.EDU Voice:619-587-4830





From: tivol-at-tethys.ph.albany.edu
Date: Wed, 01 Feb 1995 16:45:52 EST
Subject: RE: EM Lab scheduling

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Jan Coetzee asks about electronic vs. "dog-eared diary" for schedules.
Dear Jan,
We use a large desk calendar ourselves. It seems easier, since there
are frequent changes, and everyone can check it instantly. I'm sure that a
calendar page on a computer might do as well, but when a user calls, we are
not always booted up, so it would take us more time than it does with paper
and pencil. If your scopes are computer-controlled, it might save some time
and/or effort to have schedules on the same computer--e.g. the computer could
dial a user you need to contact re schedule changes--but we have not thought
this to be worthwhile for us. Sometimes low-tech works very well!
Yours,
Bill Tivol




From: tivol-at-tethys.ph.albany.edu
Date: Wed, 01 Feb 1995 17:43:19 EST
Subject: Peltier devices

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Jan Coetzee inquired about mfgrs of pltrs.
Dear Jan,
Melcor makes them. Their address, phone & fax are
1040 Spruce Street
Trenton NJ 08648-4587
United States of America (our USA)
(609) 393-4178 (phone)
(609) 393-9461 (fax)
One potential problem is that Peltiers have a limited delta-T, and can
get very expensive. I asked Melcor about setting up a cold stage for epitax-
ial deposition in a vacuum, and there was nothing which would get cold enough.
Furthermore, if you wish to thermostat the device, you need to use a cooler
which has a 100% duty cycle and output current proportional to the difference
between target and actual temperatures. We found this out when we installed
Peltiers in our darkroom as heaters/coolers to maintain developer temperature.
The intermittant current put out by the usual commercially available devices
blew the Peltiers out! Otherwise, Peltiers are marvelous devices. Good luck.
Yours,
Bill Tivol




From: WayneWNB-at-aol.com
Date: Wed, 1 Feb 1995 22:28:40 -0500
Subject: Electron Microscope Pictures

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Message-Id: {25020121201502-at-vms2.macc.wisc.edu}

G'day Subscribers...

I've received this request for help on locating micrographs
of bacteria. I could not help this individual. So is there
anyone out there that can?

Cheers....Nestor Z.
Your Friendly Neighborhood SysOp
=====================================

P.S.

I'm working on concept of adding an Image Library to the Software
Library. When it's ready for contributions I'll post
a message to the Listserver....


====================================



I need an electron microscope picture of Methanosarcina barkeri and
Methanobacterium wolfei. I called the University of California at Berkeley
and they gave me your address. They said that they don't keep a file of
their past work and would charge $300 per picture to do the work again. I'm
fairly certain that the pictures already exist somewhere. I believe that
NASA may have some of them but I don't know who to ask there . Thank you for
any help in getting any pictures of the two bacteria.

------------------




From: T.K. Wilson :      WILSONTK-at-CASMAIL.MUOHIO.EDU
Date: Thu, 2 Feb 1995 09:08:47 -500
Subject: Position Openning

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The following files have been delivered to you - please use
the eXtract command in the browser to work with them:

MARSALL.ASC (format: Text)
----------------------------------------------------------------------
Thomas K. Wilson wilsontk-at-MUohio.edu
Dept. of Botany
Miami University ! Miami was a University when
Oxford OH 45056 ! Florida belonged to Spain !
USA
513.529.4210 office
513.529.4243 fax








-------------- Enclosure number 1 ----------------
The following is posted as a favor to
Marshall University, and to any interested
applicants on the MSA-BBS. Please distribute this
message to any and all interested people you may
know.

I am not involved with this Supervisor search
in any way whatsoever (Other than having promised
to post this ASAP). Please contact Dr. W.B.
Rhoten directly at the address below (his E-mail
address is Rhoten-at-musom01.mu.wvnet.edu)




POSSITION OPEN


SUPERVISOR OF ELECTRON MICRSCOPY FACILITY


To supervise and perform day-to-day
operations of the Electron Micrscopy Facility and
assist students, research assistants, and
investigators. Participate in graduate level EM
Course.

Bachelor's degree including courses in
biological and physical sciences and at least 3
years experience in EM, or a Masters degree and at
least 3 years of relevant experience, or a Ph.D.
degree. Qualifications include comprehensive
knowledge of EM and ability to enhance educational
and research activities. Experience in image
processing and computers desired.

Qualified applicants should send a cover
letter, resume, and list of at least 2 references
to:

Dr. W. B. Rhoten
Dept. of Anatomy, Cell & Neurobiology
School of Medicine, Marshall University
Huntington, WV 25704-9388


For full consideration submit application by
March 1, 1995.

MARSHALL UNIVERSITY IS AN AFFERMATIVE
ACTION/EQUAL OPPORTUNITY EMPLOYER






From: Richard Lee :      richard_lee-at-qmgate.anl.gov
Date: 2 Feb 1995 13:01:13 -0600
Subject: subscibe

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Message-Id: {n1420392126.63194-at-qmgate.anl.gov}

subscibe 2/2/95

subscribe, PLEASE!
12:46 PM




From: richard.easingwood-at-stonebow.otago.ac.nz (Richard Easingwood)
Date: Fri, 3 Feb 1995 08:56:44 +1100
Subject: TEM:Staining glycogen in sections

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Message-Id: {199502022051.JAA12437-at-arwen.otago.ac.nz}
X-Sender: st004718-at-brandywine.otago.ac.nz
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

One of EM Unit users is studying developing Red deer. The samples we are
examining are skull and pedicle (developing antler) taken from the deer at
set time intervals over atwo year period. The samples are processed
routinely, ie glutaraldehyde fixed,decalcified, OsO4 post fixed, uranyl
acetate bloc stain, dehydrated and embedded in Agar 100 epoxy resin.

Our problem is that it now seems to be that the glycogen content is of some
significance to the investigation. Unfortunately the above process is not
ideal for showing the glycogen.
Some recently processed samples using potassium ferrocyanide with the
osmium are brilliant however we would like to enhance the glycogen in the
previous blocks.
Does anyone out there know a method to enhance "unstainable" glycogen in
ultrathin sections?
Thanks in anticipation.

Allan Mitchell
South Campus EM Unit
allan.mitchell-at-stonebow.otago.ac.nz






From: randy nessler :      rnessler-at-emiris.iaf.uiowa.edu
Date: Thu, 2 Feb 1995 14:51:56 -0600 (CST)
Subject: Site for JCPDS

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Does anyone know of a site that I can download files from the JCPDS?
Randy Nessler
rnessler-at-emiris.iaf.uiowa.edu







From: Doug Arrell :      ARRELL-at-jrc.nl
Date: Fri, 3 Feb 1995 08:31:16 GMT+0200
Subject: Re: Convert PS files

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} I am looking for a way to convert my PostScript files into "regular" image
} files. Does someone know of such siftwares on either Mac or Unix platforms?
} Any information would be appreciated.
}
Do you mean postscript or encapsulated postscript? If postscript then
one of the postscript interpreters available should do it. I use one
an a PC called GoScript that outputs to TIFF files as well as
printers. I am not sure, but you should take a look at the GNU
Ghostscript interpreter that runs on virtually anything - certainly
on unix systems. If you mean encapsulated postscript (EPS) then just
import the files into a Mac word processor such as WordPerfect on
Microsoft Word and then copy and paste to whereever you want to.
+------------------------------------+
| Dr Douglas Arrell |
| Mechanical Performance and Joining |
| Institute for Advanced Materials |
| 1755 ZG Petten |
| Netherlands |
| {ARRELL-at-JRC.NL} |
| Tel. (+31) 2246 5287 |
| Fax (+31) 2246 1917 |
+------------------------------------+




From: MicroToday-at-aol.com
Date: Fri, 3 Feb 1995 08:58:20 -0500
Subject: Bacteria Micrographs

Contents Retrieved from Microscopy Listserver Archives
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Group -
Responding to an inquiry from Nestor, Custom Medical Stock Photo is a
company which purchases micrographs and then sells them to publications, etc.
They have a considerable inventory - in microscopy, and a number "with"
bacteria.
Many readers may be interested in contacting them and explore the sale of
their micrographs. I have, of course, no financial interest in the company.

Custom Medical Storck Photo, Inc.
3819 North Southport Ave
Chicago, IL 60613
Tel.: 312-248-3200
Fax: 312-248-7427

Regards,
Don Grimes, Microscopy Today




From: Kris_Kavanau-at-dmcmail.ucsf.edu
Date: Fri, 03 Feb 1995 10:03:40 PST
Subject: Uranyl Glass/FM Stds.

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http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Message-Id: {1995Feb03.100340.2874650620-at-dmcmail.ucsf.edu}
To: Microscopy-at-aaem.amc.anl.gov (M)

Subject: Time: 9:45 AM
OFFICE MEMO Uranyl Glass/FM Stds. Date: 2/3/95
Dear Microscopists,
Does anyone have any uranyl glass, or know where it might be obtained? I
have been told that it is no longer manufactured commercially. It might be
an excellent "generic" fluorescence microscopy control.
Are there any commercially available, pre-mounted fluorescence standards
besides "MultiSpeck" from Molecular Probes? They are very convenient, but
they are not ideal for our applications as DAPI, fluorescein, and Texas Red
specific controls. Unfortunately, Flow Cytometry Standards Co. no longer
makes pre-mounted standards.
I have been managing the UCSF core flow and image cytometry facility ("Lab
for Cell Analysis") for 2 years, but I had no real QC for our 2
occasionally used fluorescence microscopes. Now I need to establish QC
protocols for 6 additional multi-user, computerized fluorescence (one
scanning confocal) microscopes in the "National Molecular Cytogenetics
Resource." I was surprised that so few standards (and journal references)
seem to be available.
Any suggestions or comments would be greatly appreciated. Thank you very
much. Kris Kavanau; kavanau-at-dmc.ucsf.edu








From: John Kloetzel :      kloetzel-at-umbc.edu (John Kloetzel)
Date: Fri, 3 Feb 1995 14:14:17 -0500
Subject: EM network

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subscribe microscopy kloetzel-at-umbc.edu

** JAK **

****************************************************************************
John A. Kloetzel, Ph.D. {kloetzel-at-umbc.edu}
Department of Biological Sciences
University of Maryland Baltimore County (UMBC)
Catonsville, MD 21228-5329 USA
Phone: (410) 455-2247 or -3913 (Lab)
FAX: (410) 455-3875
****************************************************************************








From: Dr. William Dentler, University of Kansas, Dept. of Cell Biology,
Date: Fri, 3 Feb 1995 15:24:59 -0600
Subject: fix pepper redux

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Message-Id: {m0raUmA-00011cC-at-pegasus.cc.ucf.edu}

A few months ago a thread ran on fixation and embedding pepper contaminant
artefact in biological ultrathin sections. A colleague of mine read the Pepper
Summary I compiled and sent me the following fixation protocols that he has used
successfully without pepper. Notice the first one in which glutaraldehyde,
phosphate buffer AND OSMIUM are all mixed TOGETHER!

If anyone out there wants a copy of my Pepper Summary, contact me off-line at my
e-mail and I will zip a copy out to you.

------------------------------------------------------------------------


"1. For fixing cilia in mammalian trachea, I have used an "instant fixation"
method using a combination of osmium, phosphate buffer, and glutaraldehyde - in
the cold - for many years and have never seen the pepper described in the Pepper
Summary you sent to me. Right - all that stuff in the same buffer dumped on the
tissue. Works great, but may extract a bit of actin.

"The method I used was one described in a paper by Omnoto
and Kung in the J. Cell Biol 87:33-46. I think it uses 50 mM NaPhosphate,
pH 7.2, 2% OsO4, 2% glutaraldehyde. Add the Osmium just before you add
the tissue and fix on ice for 10 min. If you want, you can remove the
black fix after 10 min and add another slug of fix for another 10 min but
that is optional.

"Omoto and Kung used it to fix Paramecium and their cilia. I have used it to
fix mammalian trachea (with their cilia). The advantage is that it seems
to "freeze" cilia in position, as opposed to glutaraldehyde, in which cells
actualy swim for a dab before either being fixed or dying (we fix cells,
we don't kill them, do we?). The osmium does not penetrate for more than
a few cell layers but, with epithelial tissue or single cells, it does
not make much difference.

"I have never tried that fix method on Chlamydomonas or Tetrahymena. Over the
last year I have done a lot of embedding of Chlamy and have never seen pepper.
For those beasts, I find that cacodylate gives the best preservation of the
cytoplasm, although others find that phosphate buffer works fine too.
I do fix with glutaraldehyde in culture medium (pretty much phosphate buffer),
overnight in glut in cacodylate, rinse a few times in cacodylate, then
into 0.5-1% OsO4 in cacodylate for 30-60 min on ice, rinse with a few
changes of water and into uranyl acetate for a few hours prior to dehydrating in
acetone and embedding in epon.

"I've also tried another method in which you fix with glut in phosphate buffer,
rinse, then incubate overnight in uranyl acetate (in water) at 70 degrees
C. It works on Chlamy and avoids osmium. It is supposed to eliminate the
need for poststaining of sections, but I did not find this to be the
case. I believe that I once read that the reason for staining sections is
to put a dab of stain on structures at the last surface of the plastic
that the beam sees before blasting through the objective lens. I don't
know, but, for Chlamy, I usually use the more old fashioned method that I
gave you above. Pepper does not seem to be one of my problems."
------------------------------------------------------------------------
Contributed to MICROSCOPY by:

--

Gib Ahlstrand, MMS Newsletter Editor
Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
495 Borlaug Hall, St. Paul, MN 55108 (612)625-8249
612-625-9728 FAX, giba-at-puccini.crl.umn.edu






From: tivol-at-tethys.ph.albany.edu
Date: Fri, 03 Feb 1995 18:23:23 EST
Subject: Re: TEM film

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Dear Lucille,
I just got a number from Kodak for technical questions, but they could
probably direct you to a distributer. It is (800) 242-2424 x19. We usually
use a local vendor, National Graphics, but I have not noticed a great range
of prices with other vendors. Good luck.
Yours,
Bill Tivol




From: Dr. Edmund Glaser :      eglaser-at-umabnet.ab.umd.edu
Date: Fri, 3 Feb 1995 22:12:08 -0500 (EST)
Subject: Re: TEM film

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I am not aware that "cheap" is an a.k.a. for "reliable". Please be kind
to the English language.

On Fri, 3 Feb 1995, Lucille A. Giannuzzi wrote:

} Can anyone recommend a reliable (a.k.a. cheap) U.S. vendor for TEM film?
}
} Thanks in advance,
} Lucille Giannuzzi
}
}
} *************************************************************************
} Lucille A. Giannuzzi, Ph.D.
}
} Dept. of Mechanical and Aerospace Eng. phone (407) 823-5770
} University of Central Florida fax (407) 823-0208
} 4000 Central Florida Blvd. email lag-at-pegasus.cc.ucf.edu
} Orlando, FL 32816-2450 USA
} *************************************************************************
}
}
}
}




From: Karpura V Kommineni :      komminen-at-student.msu.edu
Date: Sun, 5 Feb 1995 12:32:08 -0500 (EST)
Subject: immunolabeling of wood

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Message-Id: {9502051732.AA72211-at-student1.cl.msu.edu}

DDoes anyone know about work done on immunolabelling of wood tissue of fruit
trees. I'm interested in any kind of information I can get on fixation,
embedding and the labelling procedure. I plan to be using ProteinA-colloidal
gold to tag the antibody.I'll be using a confocal microscope for this study.If
anyone knows any work done in this area could you please send me the
references. Thank you in advance.
my email address: komminen-at-student.msu.edu






From: Ian Hall :      hall-at-me.udel.edu
Date: Mon, 6 Feb 1995 08:31:26 -0500 (EST)
Subject: WDS Software

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Dear Fellow Microscopists,
We have recently acquired a scanning electron microscope with a
four crystal Wavelength Dispersive Spectrometer but the associated
computer system is rather old and probably not worth re-activating.
I know that there is software for the Macintosh, such as
"D.T.S.A." and "Flame", for ENERGY Dispersive Spectroscopy but my question
is "Is there any (Mac) software out there which can handle W(AVELENGTH)DS
spectral acquisition and processing?".
Any leads would be very much appreciated. Thanks.

Rick Hall
Materials Science Program
University of Delaware







From: Not Specified :      Kris_Kavanau-at-dmcmail.ucsf.edu
Date: Fri, 03 Feb 1995 10:03:40 PST
Subject: Uranyl Glass/FM Stds.

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Reply to: RE} Uranyl Glass/FM Stds.
Hi Kris,
Uranium glass slides can be purchased from:

Newport Industrial Glass, Inc.
1631 Monrovia Ave.
Costa Mesa, CA 92627
Tel: 714-642-9980
Fax: 714-645-6800
Contact person: Bill Larsen (you can tell him I sent you).
Sold as a 6.5x6.5" sheet (you can specify 1 mm height), so you may want to form
a "consortium" to have Newport pre-cut a sheet to slide size (nominal extra
cost, but your lab only needs one or two slides). If there is a lot of
interest, my company may start selling single slides.

As for references and the Shading Correction equation: please see my article in
the 11/94 issue of Journal of NIH Research 6(11): 80 (usual Internet
disclaimer: yes, that is an ad from my company on the facing page). Also look
at Jericevic et al (1989) Methods in Cell Biology 30:47-83.

MutliSpeck beads: The Molecular Probes pre-mounted slide kits should be ideal
for DAPI and Fluorescein. I believe they were optimized for Rhodamine, but
should still work ok for Texas Red. If your problem is with mounting, Mol.
Probes now sells the beads in solution, so you can 'sprinkle' some on your
specimens. If you have a different problem with the current MultiSpeck's, Mol.
Probes may be able to work something out for you.

Sorry, but I usually buy my reference material from Mol. Probes and don't keep
close track of other slide manufacturers.

Sincerely,

Dr. George McNamara
Universal Imaging Corporation
George_M-at-Image1.com
--------------------------------------

Subject: Time: 9:45 AM
OFFICE MEMO Uranyl Glass/FM Stds. Date: 2/3/95
Dear Microscopists,
Does anyone have any uranyl glass, or know where it might be obtained? I
have been told that it is no longer manufactured commercially. It might be
an excellent "generic" fluorescence microscopy control.
Are there any commercially available, pre-mounted fluorescence standards
besides "MultiSpeck" from Molecular Probes? They are very convenient, but
they are not ideal for our applications as DAPI, fluorescein, and Texas Red
specific controls. Unfortunately, Flow Cytometry Standards Co. no longer
makes pre-mounted standards.
I have been managing the UCSF core flow and image cytometry facility ("Lab
for Cell Analysis") for 2 years, but I had no real QC for our 2
occasionally used fluorescence microscopes. Now I need to establish QC
protocols for 6 additional multi-user, computerized fluorescence (one
scanning confocal) microscopes in the "National Molecular Cytogenetics
Resource." I was surprised that so few standards (and journal references)
seem to be available.
Any suggestions or comments would be greatly appreciated. Thank you very
much. Kris Kavanau; kavanau-at-dmc.ucsf.edu











From: SiSTek-at-aol.com
Date: Mon, 6 Feb 1995 13:53:07 -0500
Subject: subscribe

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Subscribe, please.

Thanks,

Mark Anderson, SiSTek




From: Deborah Holmberg :      dlholmberg-at-ucdavis.edu
Date: Mon, 6 Feb 1995 11:01:33 -0800 (PST)
Subject: Scanning 95 meeting

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The organizers of the 28-31 March 1995 Scanning 95 meeting want about 25
student volunteers to run the slide projectors for the sessions in
exchange for full meeting registration ($275). Please contact:
Mary K. Sullivan
FAMS, Inc
P.O. Box 832
Mahwah, New Jersy
07430,0832

or leave me a message.
Debe Holmberg e-mail {dlholmberg-at-peseta.ucdavis.edu}
Lab 916-752-9021
FAX 916-752-4604




From: David Leaffer (415)852-1828 :      David.Leaffer-at-syntex.com
Date: 06 Feb 1995 11:56:02 -0800 (PST)
Subject: TEM Calibration

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Return-receipt-to: David.Leaffer-at-syntex.com
Registered-mail-reply-requested-by: David.Leaffer-at-syntex.com

I am going to be working on an TEM project that will be under "GLP" . GLP are
guidlines for doing certain experiments for the FDA (I think the equivalent in
Europe is ISO 9000). I was wondering if anyone out there is doing TEM under
these guidlines? And if so what are they using for magnification calibration.
I do not believe that there are any vendors who can supply a certified
magnification standard for TEM, which, I think is required for GLP.

Thanks,
David Leaffer
Syntex Research
david.leaffer-at-syntex.com





From: Deborah Holmberg :      dlholmberg-at-ucdavis.edu
Date: Mon, 6 Feb 1995 16:38:54 -0800 (PST)
Subject: Scanning 95 meeting

Contents Retrieved from Microscopy Listserver Archives
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The organizers of the 28-31 March 1995 Scanning 95 meeting want about 25
student volunteers to run the slide projectors for the sessions in
exchange for full meeting registration ($275). Please contact:
Mrs. Mary K. Sullivan
FAMS. Inc.
P.O. Box 832
Mahwah, NJ 07430-0832


or leave me a message.
Debe Holmberg
Lab 916-752-9021
Fax 916-752-4604
dlholmberg-at-peseta.ucdavis.edu





From: richard.easingwood-at-stonebow.otago.ac.nz (Richard Easingwood)
Date: Wed, 8 Feb 1995 13:50:28 +1100
Subject: Academic role

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Dear All,
We are are multi-user electron microscope facility which has an extensive
range of equipment and uses a wide range of techniques.
Five technical staff from three contributing University departments are
employed full-time to undertake for work coming from both inside and
outside the University.

The role of the 'academic in charge' of the facility is shortly to come up
for reassessment and so it is a pertinent time for us to reconsider what
that role should entail.
We are looking for feedback from other individuals as to what they see the
contribution of an academic in this environment should be.
Any opinions/suggestions?






From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Tue, 7 Feb 1995 21:59:52 -0600 (CST)
Subject: Post Doc In Tribology

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From: wabutter-at-ix.netcom.com (Wayne A. Buttermore)
Date: Tue, 7 Feb 1995 20:03:15 -0800
Subject: subscribe

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Message-Id: {9502071626.AA16923-at-riker.ml.wpafb.af.mil}

Subscribe





From: Peter Goodhew :      goodhew-at-liverpool.ac.uk
Date: Wed, 8 Feb 1995 08:43:25 GMT
Subject: Re: Academic role

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} The role of the 'academic in charge' of the facility is shortly to come up
} for reassessment and so it is a pertinent time for us to reconsider what
} that role should entail.
} We are looking for feedback from other individuals as to what they see the
} contribution of an academic in this environment should be.
Easy -
1. Keep abreast of all techniques which a) you have, b) exist, and c) potentially exist.
2. Be an expert practionioner of two or more of these. Publish a lot in your own name.
3. Give advice on the application of all techniques and on the high-level interpretation of all results. Publish
jointly with others.
4. Raise funds to replace equipment and to buy new techniques as they become applicable.
5. Make sure all users publish, and tell you about it!
6. Establish a reputation as a scientist in some major subject area, not just as a microscopist. Publish a lot.
7. Keep friendly with the heads (or budget controllers) of all potential user departments.
8. Get to know lots of people in your institution by playing sport/drinking/etc with them.
9. In your spare time, publish some more.

I offer this advice after 20 years of running such a facility!

Peter Goodhew



----------------------------------------------------------------------------------------------------------
Professor Peter J Goodhew, Department of Materials Science & Engineering
University of Liverpool
LIVERPOOL Fax (44) (0)51 794 4675
L69 3BX, UK Tel (44) (0)51 794 4665 (secretary Debra)
----------------------------------------------------------------------------------------------------------
inter alia: Director of the MATTER project for educational software
----------------------------------------------------------------------------------------------------------






From: Philip.P.Edge-at-ioppl.co.uk (Tel 0272 297481)
Date: Wed, 8 Feb 1995 11:11:01 +0000
Subject: Subscribe

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From: Philip.P.Edge-at-ioppl.co.uk (Tel 0272 297481)
Date: Wed, 8 Feb 1995 11:14:29 +0000
Subject: Bioimaging

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Institute of Physics Publishing
Techno House Tel: +44 272 297481
Redcliffe Way Fax: +44 272 294318
Bristol
BS1 6NX England Telex: 449149 INSTP G


E-mail Contact Details
----------------------
Internet : {mailbox} -at-ioppublishing.co.uk
Janet : {mailbox} -at-uk.co.ioppublishing
X400 : /s= {mailbox} /o=ioppl/prmd=iopp/admd=0/c=gb/

IOPP Internet services
----------------------
Gopher : gopher.ioppublishing.com
WWW Url : http://www.ioppublishing.com

Dear Moderator

I am the Publisher of the journal Bioimaging which I hope you have heard
of. I would like to place information about the journal, including tables
of contents, on your bulletin board. Will this be possible and how should I
do it?

Best wishes, Philip Edge.




From: jester-at-crnjjsgi.swmed.edu (James V. Jester)
Date: Wed, 08 Feb 1995 12:10:01 -0600
Subject: Lab Design

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Several years ago there appeared an article in Science discussing
laboratory space design and a recent "New" model being implemented in
England. Does anyone remember this article, and what has happened
to the English experiment?






From: Marc Brande :      brande-at-sdsc.edu
Date: Wed, 8 Feb 1995 10:04:15 -0800 (PST)
Subject: Timelapse Cell Culture Movies

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Microscopy List {microscopy-at-aaem.amc.anl.gov} ,
Functional Neuroimaging List {lat-at-po.cwru.edu} ,
Confocal Microscopy List {confocal-at-ubvm.bitnet} ,
Cell Bio List {cellbiol-at-net.bio.net}
Message-Id: {Pine.3.05.1.9502081015.B14211-a100000-at-pauline.sdsc.edu}
Mime-Version: 1.0
Content-Type: TEXT/PLAIN; charset=US-ASCII

Can Anyone point me to sources (free to minimal cost) of analog (VCR tape)
or digital timelapse movies of cells in culture? This is not for
commercial use, only presentation demonstration. Of course I would credit
each source in the presentation. Thanks in advance for any help you can give.

Marc

Marc C. Brande, M.S. SD3D Email List:3D Imaging
San Diego 3D Imaging Group To subscribe/unsubscribe:listserv-at-bobcat.etsu.edu
3840 Camino Lindo To post a message:sd3d-at-bobcat.etsu.edu
San Diego, CA 92122 Email:BRANDE-at-SDSC.EDU Voice:619-587-4830





From: evansnd-at-ornl.gov (Neal D. Evans)
Date: Wed, 8 Feb 1995 13:17:21 -0500
Subject: Subscribe

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Subscribe Microscopy evansnd-at-ornl.gov

Dr. Neal D. Evans
Shared Research Equipment Program
Oak Ridge National Laboratory
Building 5500, MS 6376, Oak Ridge, TN 37831-6376
voice(615-576-4427) fax(615-574-0641) email(evansnd-at-ornl.gov





From: stuw-at-lanl.gov (Stuart Wright)
Date: Wed, 8 Feb 1995 11:22:29 -0700
Subject: Reconditioned SEMs

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Message-Id: {9502081818.AA09732-at-mustang.mst6.lanl.gov}
X-Sender: stuw-at-mustang.mst6.lanl.gov
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Is anyone aware of a company that sells reconditioned SEMs?


+---------------------------------------------------------+
| Stuart Wright Los Alamos National Lab, MST-6 |
|.........................................................|
| Mail Stop G770 phone: (505) 665-3647 |
| Los Alamos, NM 87545 fax: (505) 667-5268 |
+---------------------------------------------------------+






From: Daniel E. Sampson :      des-at-rupture.ucsc.edu
Date: Wed, 8 Feb 1995 11:22:29 -0700
Subject: Reconditioned SEMs

Contents Retrieved from Microscopy Listserver Archives
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Is anyone aware of a company that sells reconditioned SEMs?


+---------------------------------------------------------+
| Stuart Wright Los Alamos National Lab, MST-6 |
|.........................................................|
| Mail Stop G770 phone: (505) 665-3647 |
| Los Alamos, NM 87545 fax: (505) 667-5268 |
+---------------------------------------------------------+




------ Forwarded message ends here ------


*******************************************************************
Daniel E. Sampson dsampson-at-earthsci.ucsc.edu
Instrumentation Specialist Phone: (408) 459-4992
Earth Sciences FAX: (408) 459-3074
University of California
Santa Cruz, CA 95064
*******************************************************************




From: Elinor Solit :      cambrex-at-world.std.com
Date: Wed, 8 Feb 1995 17:34:11 +0001 (EST)
Subject: Re: Reconditioned SEMs

Contents Retrieved from Microscopy Listserver Archives
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Stuart,
Have you tried contacting the instrument manufacturers themslves? I
believe that JEOL and Hitachi, for instance, may sell the
reconditioned SEMs that comes in on trade-ins. Failing that, give
us a call, we'll try to direct you to more sources.

Ellie Solit,
Publisher of MICROSCOPE TECHNOLOGY & NEWS AND
THE MICROSCOPE BOOK


On Wed, 8 Feb 1995, Stuart Wright wrote:

} Is anyone aware of a company that sells reconditioned SEMs?
}
}
} +---------------------------------------------------------+
} | Stuart Wright Los Alamos National Lab, MST-6 |
} |.........................................................|
} | Mail Stop G770 phone: (505) 665-3647 |
} | Los Alamos, NM 87545 fax: (505) 667-5268 |
} +---------------------------------------------------------+
}
}




From: richard.easingwood-at-stonebow.otago.ac.nz (Richard Easingwood)
Date: Thu, 9 Feb 1995 16:43:19 +1100
Subject: Academic role

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Message-Id: {199502090437.RAA16024-at-arwen.otago.ac.nz}
X-Sender: st004718-at-brandywine.otago.ac.nz
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Dear All,
Further to my message of 8.2.95, thank you very much to those who have
responded so candidly to my request for people's feelings and experiences
on this topic. I appreciate that it can be a sensitive issue, not helped
when one is replying to someone who doesn't even identify themselves
properly.
As I neglected to state who I am and where I work (I changed computers days
ago and forgot to put the automatic signature on - the ramifications of
this I am just becoming aware of...) that info now follows.
Maybe now I'll get some more replies...

Richard Easingwood
South Campus Electron Microscope Unit
Otago Medical School
PO Box 913
Dunedin
NEW ZEALAND

Telephone: 64-03-479 7301
Facsimile: 64-03-479 7254






From: Kathy Walters :      kwalters-at-emiris.iaf.uiowa.edu
Date: Thu, 9 Feb 1995 07:17:27 -0600 (CST)
Subject: Neg stain of lipids

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Dear Fellow Microscopists,

I have been asked to do particle sizing of a 10% fat immulsion (pH 8.0)
and had hoped to be able to do a very quick negative staining procedure as
this may need to be run frequently. I toyed with the idea of freeze
fracture, but the time involved is not convenient for lots of runs. Has
anyone tried this? What stains would you suggest? Is Osmium
vaper fixation nessecary?

Thanks for any help!
Kathy Walters






From: stuw-at-lanl.gov (Stuart Wright)
Date: Thu, 9 Feb 1995 09:06:22 -0700
Subject: reconditioned SEMs

Contents Retrieved from Microscopy Listserver Archives
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Message-Id: {199502091342.AA01519-at-mail.mmmg.com}

Is anyone aware of a company that sells reconditioned SEMs?



+---------------------------------------------------------+
| Stuart Wright Los Alamos National Lab, MST-6 |
|.........................................................|
| Mail Stop G770 phone: (505) 665-3647 |
| Los Alamos, NM 87545 fax: (505) 667-5268 |
+---------------------------------------------------------+






From: Jay Jerome :      jjerome-at-isnet.is.wfu.edu
Date: Thu, 9 Feb 1995 11:10:54 -0500 (EST)
Subject: Re: Neg stain of lipids

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We have considerable experience with negative stain of phospholipid
vesicles, lipoproteins, and triacylglyceride emulsions. In these
circumstances fixation is not critical, and in fact can produce
artefacts. PTA stain seems to work best. We concentrate our sample on
grid by drying multiple drops under gentle nitrogen gas stream prior to
negative stain. This avoids clumping in the suspension. Some sizing
artefacts can occur as dryed particles of large size can deform slightly.
See Ganz et al, 1991, J Lipid Res 31:163 for nice discussion of this.
Good luck-


Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-isnet.is.wfu.edu *
**************************************************************

On Thu, 9 Feb 1995, Kathy Walters wrote:

} Dear Fellow Microscopists,
}
} I have been asked to do particle sizing of a 10% fat immulsion (pH 8.0)
} and had hoped to be able to do a very quick negative staining procedure as
} this may need to be run frequently. I toyed with the idea of freeze
} fracture, but the time involved is not convenient for lots of runs. Has
} anyone tried this? What stains would you suggest? Is Osmium
} vaper fixation nessecary?
}
} Thanks for any help!
} Kathy Walters
}
}
}




From: tivol-at-tethys.ph.albany.edu
Date: Thu, 09 Feb 1995 12:06:33 EST
Subject: Re: Neg stain of lipids

Contents Retrieved from Microscopy Listserver Archives
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Dear Kathy,
Staining is not really my field, but I'd suggest a water-soluble heavy
metal and NO osmium. The Os would only dissolve in the lipid and reduce con-
trast. If possible, looking at a frozen, hydrated (or lyophyllized in-situ)
specimen would be best. You don't specify either the matrix of the emulsion
(I assume aqueous) nor the technique to be used (I assume TEM), but a possible
protocol would be to add the stain to the emulsion and rapidly freeze, then
cryo-section, examine on a Friday, raise the temp to ~-90C, come in Saturday
and raise the temp to -80C, Sunday to -70C, and look at the freeze-dried spec-
imen Monday. If you can do the particle sizing from the frozen-hydrated spec-
imen, the last steps can be omitted. Keeping the stain strictly in the aqueous
phase should prevent size changes, etc. in the lipid drops. Good luck.
Yours,
Bill Tivol




From: jacobb-at-ux5.lbl.gov
Date: Thu, 9 Feb 1995 13:50:06 -0800
Subject: Re: Reconditioned SEMs

Contents Retrieved from Microscopy Listserver Archives
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Stuart,

E.J. Fjeld Co,
3 Executive Park Drive
North Billerica MA 01862
Phone 508-667-1416

Provides reconditioned AMRAY microscopes. They also make special stages and
accessories. They've been around for a long time and are reliable.

Jacob Bastacky, MD
1-116
Lawrence Berkeley Laboratory EMail: sjbastacky-at-lbl.gov
University of California Phone: (510) 486-4606
Berkeley, California 94720 Fax: (510) 486-4750





From: Elinor Solit :      cambrex-at-world.std.com
Date: Thu, 9 Feb 1995 15:20:13 +0001 (EST)
Subject: Re: reconditioned SEMs

Contents Retrieved from Microscopy Listserver Archives
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Stuart, my attempts to reply to your question by email have resulted in 6
notices of undelivered mail. I left a voice message on your phone this
morning. I think we can help you in your search.

Regards,
Ellie Solit, Publisher/Executive Editor of Microscope Technology & News
and The Microscope Book, a Smart catalog.


On Thu, 9 Feb 1995, Stuart Wright wrote:

} Is anyone aware of a company that sells reconditioned SEMs?
}
}
}
} +---------------------------------------------------------+
} | Stuart Wright Los Alamos National Lab, MST-6 |
} |.........................................................|
} | Mail Stop G770 phone: (505) 665-3647 |
} | Los Alamos, NM 87545 fax: (505) 667-5268 |
} +---------------------------------------------------------+
}
}




From: Jean Armour Polly :      jpolly-at-nysernet.ORG
Date: Thu, 9 Feb 1995 13:00:28 -0500
Subject: Apple QuickTime Conferencing

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Message-ID: {95020916094142E.RQDA-at-USCN.USCN.UGA.EDU} (UMass-Mailer 4.04)
Neuroscience List {neur-sci-at-net.bio.net} ,
Microscopy List {microscopy-at-aaem.amc.anl.gov} ,
Digital Video List {digvid-l-at-ucdavis.edu} ,
Confocal Microscopy List {confocal-at-ubvm.bitnet} ,
Cell Bio List {cellbiol-at-net.bio.net}
Message-Id: {Pine.3.05.1.9502091131.A22203-e100000-at-pauline.sdsc.edu}
Mime-Version: 1.0
Content-Type: TEXT/PLAIN; charset=US-ASCII

I thought this post should be quickly disseminated.

Marc C. Brande, M.S. SD3D Email List:3D Imaging
San Diego 3D Imaging Group To subscribe/unsubscribe:listserv-at-bobcat.etsu.edu
3840 Camino Lindo To post a message:sd3d-at-bobcat.etsu.edu
San Diego, CA 92122 Email:BRANDE-at-SDSC.EDU Voice:619-587-4830

---------- Forwarded message ----------


THE FOLLOWING RELEASE MOVED OVER PR NEWSWIRE ON TUESDAY, FEBRUARY 7, 1995 AT
11:42 AM, PST.


Contact:
Julie Karbo
Stirling & Cohan
(415) 513-0974
e-mail: jkarbo-at-applelink.apple.com

Brooke Cohan
Stirling & Cohan
(415) 513-0973
e-mail:cohan-at-applelink.apple.com

Apple Announces QuickTime Conferencing
Open, Cross Platform Conferencing, Collaboration and Multimedia
Communications Technology

SAN FRANCISCO, California--February 7, 1995--Apple Computer today
announced a cross platform conferencing, collaboration and
multimedia communications technology that allows personal computer
users to share real-time information, images and sound anywhere in
the world. Apple is currently making the technology, called
QuickTime Conferencing, available to corporate allies who plan to
create or have announced they are creating end user applications
based on the technology. QuickTime Conferencing is a standards-
based architecture that allows users to:

-- video conference and collaborate--to share and annotate text,
images, screen capture, sound, video and virtual scenes real-time
among fellow conference participants in a variety of locations
worldwide. QuickTime Conferencing allows users to record
conversations and transform those conversations into QuickTime
movies. All of this can be done on a variety of networks such as
an Integrated Services Digital Network (ISDN), the worldwide
internet, local area and wide area networks and Asynchronous
Transfer Mode (ATM) networks. QuickTime Conferencing can be used
by a number of simultaneous users, the total number being only by
available network bandwidth.

-- conduct cross platform video conferencing connectivity
between Macintosh computers, PCs, UNIX systems and room-based
conferencing systems through the use of the H.320 worldwide
teleconferencing standard.

-- broadcast and view multimedia content--digital audio, music
and video on a local or wide area network.

Through alliances QuickTime Conferencing technology is expected to
yield product bundles such as:
-- Apple Media Conference Kit--Consisting of the QuickTime
Conferencing system extension, the Apple Media Conference
application and a high quality, color video camera.
-- Apple Media Conference Pro Kit--Consisting of the QuickTime
Conferencing system extension, the Apple Media Conference
application, a color video camera and an H.320 codec/ISDN adapter
board. Being developed by Sagem/SAT, a leading international
communications product company, the board is designed to allow
interoperability between platforms (Power Macintosh to Macintosh,
PC, UNIX and room systems) and full-screen image sharing.
--Complete Media Conference System--Consisting of an Apple Media
Conference Kit, a Power Macintosh 7100 AV, a 17 inch color
monitor, external speakers and a keyboard.

Because QuickTime Conferencing is software-based, it is easily
incorporated into new and existing third party products. As such,
Apple believes that QuickTime-compatible products could yield
extremely affordable prices:
-- Apple Media Conference Kit--under $200
-- Apple Media Conference Pro Kit--under $1,750
-- Complete Media Conferencing System--under $6,000

Apple is working with a wide range of companies including telcos,
network, software and hardware providers and developers to provide
a range of solutions that take advantage of the benefits of
QuickTime Conferencing (see associated releases). These allies
have announced that they expect to make products available in the
second quarter of 1995.
From the home office to university campuses to the multinational
enterprise network, QuickTime Conferencing will allow users to
communicate with people across the country or across the world.
Users won't have to worry about whether their hardware equipment,
networking equipment and applications are compatible with the
solutions being used on the other end of the network line.
QuickTime Conferencing is designed to be fully operational with
H.320 standards-based systems.
"The introduction of QuickTime Conferencing will not only extend
Apple's leadership in multimedia, but will make an important
difference in the video conferencing and collaboration market,"
said Rick Shriner, vice president of Apple's Core Technologies
Group. "Our goal in designing QuickTime Conferencing was to
develop a solution that allowed people the opportunity to
communicate and collaborate. By making it open in every sense of
the word, our users can metaphorically break down the walls of
their homes, schools and offices and expand the boundaries of
their lives."
QuickTime Conferencing users can have access to people,
information, sights and sounds that could never be combined
before. For example:
-- An author in Tokyo, Japan and her publisher in San Francisco,
California can view and discuss cover art for a new novel. They
can each view the design at several different angles, change
the visual perspective of the artwork, and annotate the image and
accompanying text for the other to see.
-- A sixth grade class in Dallas, Texas can discuss and view the
effects of global warming with an environmental scientist at U.C.
Berkeley's Lawrence Labs in California by using QuickTime
Conferencing over the internet.
-- A special effects producer in Hollywood, California can take a
movie director on the East Coast through a virtual tour of a
proposed set design. While the producer records their discussion
as a QuickTime movie, the director can pan around the scene, zoom
in to look at props and view the set design from a variety of
angles.
-- A breast cancer patient and her doctor in Fargo, North Dakota
can consult with a leading oncologist in Boston, Massachusetts on
her prognosis and course of treatment. The Boston physician can
view her mammograms and annotate her medical chart as they
converse.
-- A CEO's company-wide address can be broadcast for easy viewing
by all employees at their personal desktop.

Because QuickTime Conferencing allows for sharing of multimedia
data and reduces the time and expense of travel, it allows people
to be more productive than ever before.
"In the past people found video conferencing easy to resist
because prices were high and the number of people they could
communicate with was extremely limited," said Rick LeFaivre,
senior vice president of the Apple Technology Group. "Now for
what we expect to be very aggressive prices, people can conduct a
media conference with virtually anyone, anywhere in the world. A
Power Macintosh QuickTime Conferencing user can share QuickTime VR
(virtual reality) images, annotate text documents and share digital
music over networks from basic rate ISDN to the internet to ATM."
Because QuickTime Conferencing is a software-based architecture,
application developers, communications providers and hardware
vendors can easily develop compatible solutions. For example,
Crosswise Corporation, the maker of Face to Face, a cross-platform
document conferencing application, developed a QuickTime
Conferencing-compatible version of their software in just one
month. A QuickTime Conferencing compatible application shares the
interface of other QuickTime Conferencing-enabled third party
applications, so customers can begin using applications quickly
and easily.
QuickTime Conferencing is based on Apple's award winning QuickTime
technology. It is a conferencing architecture which allows
support for both industry standards such as H.320, as well as
proprietary architectures, and codecs such as Indeo by Intel
Corporation. QuickTime Conferencing is transport, compression and
media-device independent. Apple's built-in AV capabilities
combined with the performance of the PowerPC RISC architecture,
make it easy for users to make multimedia connections with others
on the information superhighway almost as soon as they pull
QuickTime Conferencing out of the box.
"Having QuickTime Conferencing available in my home, office, and
studio literally allows me to be in multiple locations at one
time--it's the next best thing to having a Star Trek transporter,"
said Los Angeles-based screenwriter and multimedia special effects
consultant Michael Backes, co-author of the screenplay for
Jurassic Park and other motion pictures. "Within the next few
months, I'll be counting on QuickTime Conferencing as the backbone
for my business."
"The short and sweet of QuickTime Conferencing is that it requires
less network bandwidth and uses innovative technology," says Matt
Ghourdjian, National Director of Technology at Howrey & Simon, a
300-lawyer law firm serving Fortune 50 clients. Howrey & Simon
intends to use the product to send QuickTime movies of depositions
and re-enactments for lawyers to use in court; for live document sharing;
for consultation between partners; and to conduct tours of the firm's
Washington, DC office from Los Angeles. "It's simply outstanding," says
Chris Masten, Howrey & Simon's Technical Litigation Support Manager.
To use the Apple Media Conference Kit on the Macintosh, users need
at least 16 Megabytes of RAM, a 68040 or PowerPC-based Macintosh,
System 7.5, a network interface such as Ethernet, ISDN, Token
Ring, and optionally the ability to digitize audio and video using
the built-in AV subsystem or a third party digitizer card. To use
the Apple Media Conference Pro Kit on Macintosh, users need at
least 16 Megabytes of RAM, an AV PowerPC-based Macintosh and an
ISDN connection. To communicate with QuickTime Conferencing users
from the PC and other platforms, users will need an H.320
compatible codec on their machine, available from a variety of
vendors. QuickTime Conferencing technology is currently under
development and products using the technology have not yet been
completed. Apple will provide pricing and availability
information when products are completed and ready for release.
Apple Computer, Inc., a recognized pioneer and innovator in the
information industry, creates powerful solutions based on easy to
use personal computers, servers, peripherals, software, online
services, and personal digital assistants. Headquartered in
Cupertino, California, Apple (NASDAQ:AAPL) develops, manufactures,
licenses and markets products, technologies and services for the
business, education, consumer, scientific & engineering and
government markets in over 140 countries.


-30-

Apple, the Apple logo, QuickTime and Macintosh are registered
trademarks and Power Macintosh is a trademark of Apple Computer,
Inc. Additional company and product names may be trademarks or
registered trademarks of the individual companies and are
respectfully acknowledged.

END

Applelink pathway:
News Break
Apple & Industry News
PR Express
Apple Press Releases
2/7/95













From: Dascorr-at-aol.com
Date: Fri, 10 Feb 1995 10:38:26 -0500
Subject: Reconditioned SEMs

Contents Retrieved from Microscopy Listserver Archives
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I know of a company that reconditions old AMRAY SEMs. The owner used to work
at AMRAY before starting his own company. Company is E.J. Feld located in
Massachusetts. I can give you the phone on Monday, 2/13 when I return to
work.
Dr. David A. Shifler
Powell Labs Ltd.
Baltimore, MD 31231
(410) 327-3500
(410) 327-7506 (FAX)




From: Liang, Long :      LLIANG-at-is.Arco.COM
Date: 10 Feb 1995 11:07:11 CST
Subject: Books-- EM & Geology ?

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Message-Id: {MACMS.LLIANG.061405110095041FMACMS-at-IS.ARCO.COM}


Dear Microscopists,

Does anyone know if there is any recent books about applications of
microbeam techniques to mineralogy and petrology?

The one I have was published by Mineralogical Association of Canada
(Short Course in Microbeam Techniques) in May 1976.

Thanks in advance.

Long LIang
ARCO EPMA/SEM Lab
PLano, TX






From: JoRita Jordan :      jjordan-at-world.std.com
Date: Fri, 10 Feb 1995 13:27:19 +0001 (EST)
Subject: TEM comments sought

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TEM users:

Thanks to all the replies to my recent TEM survey, I am well on my way to
preparing the survey for publication. I need some information -- I'm a
chemist, not a microscopist -- What do TEM users see as important trends in
TEM? New technology? Important applications? Is there other technology that
is replacing TEM in any way. How important are accessories like EDS and
EELS? For what uses?

Any comments on today's TEM would be greatly appreciated -- I'll send a copy
of the survey to any contributor.

Jo Rita Jordan
Analytical Consumer




From: DCROMEY-at-CCIT.ARIZONA.EDU
Date: Fri, 10 Feb 1995 13:50:01 -0700 (MST)
Subject: Phone # for Presetation Technol. needed

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Does anyone in the group have the phone number for PRESENTATION
TECHNOLOGY? We are in the information gathering stage for the purchase
of a 35mm slide maker and the phone number we have (408-774-3733) is not
correct.

Thanks for your help.

Doug


Douglas W. Cromey, M.S.
Cell Biology and Anatomy
Arizona Health Sciences Center
1501 N. Campbell Ave.
Tucson, AZ 85724
(602)626-2824 dcromey-at-ccit.arizona.edu













From: Peter D. Barnett :      pbarnett-at-crl.com
Date: Fri, 10 Feb 1995 22:41:51 -0800 (PST)
Subject: Phone # for Presetation Technol. needed

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help

Peter D. Barnett - Forensic Science Associates - Richmond CA
pbarnett-at-crl.com VOICE: 510-222-8883 FAX: 510-222-8887






From: timonf-at-earth.ruu.nl (Timon Fliervoet)
Date: Mon, 13 Feb 1995 08:24:35 +0100
Subject: Re: Books-- EM & Geology ?

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Message-Id: {n1419587852.54350-at-qmgate.anl.gov}

} Dear Microscopists,
}
} Does anyone know if there is any recent books about applications of
} microbeam techniques to mineralogy and petrology?
}
} The one I have was published by Mineralogical Association of Canada
} (Short Course in Microbeam Techniques) in May 1976.
}
} Thanks in advance.
}
} Long LIang
} ARCO EPMA/SEM Lab
} PLano, TX


Try:
McLaren, A.C., 1991, TEM of minerals and rocks, Cambridge University Press,
Cambridge

Boland, J.N. & FitzGerald, J.D. (eds), 1993, Defects and processes in the
solid state: geoscience applications, Developments in Petrology, Vol 14,
Elsevier, Amsterdam

Buseck, P.R. (ed), 1992, Minerals and reactions at the atomic scale: TEM,
Mineralogical Society of America, Reviews in Mineralogy, vol 27.

Cheers Timon

------------------------------------------------
Timon Fliervoet, Timonf-at-earth.ruu.nl, Faculty of Earth Sciences, Department
of Geology, Utrecht University, P.O. Box 80.021 3508 TA Utrecht, the
Netherlands.
tel: ++31 30 - 535054, fax: ++31 30 - 537725






From: SiSTek-at-aol.com
Date: Mon, 13 Feb 1995 09:29:32 -0500
Subject: Need TEM analysis

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Help please!! SiSTek is looking for TEM analytical services in the
southwestern US, preferably in the Phoenix metropolitan area where we are
located. We are a company that provides consultant services to a number of
manufacturers of Si-related deposition systems and need *local* (turnaround
time and iterative analysis is important for our clients) TEM support. We
believe there is a company in the Phoenix area offering TEM services, but
can't find the name. Does anyone know the name/contact?

Many thanks,

Mark Anderson, SiSTek




From: ckblack-at-dow.com (U096585)
Date: Tue, 14 Feb 1995 11:03:19 -0500
Subject: mycoplasm in cell culture

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Hello folks.....

This may not be the correct forum for the
following query, however, any info out there would
be greatly appreciated.

We are interested in microscopy techniques,
testing procedures, staining , etc., for detection
of mycoplasm in cell cultures.

Thankyou in advance.

Cary Black (ckblack-at-dow.com)
Dave Williams

The Dow Chemical Company




From: Doug Davis :      doug_davis-at-maillink.berkeley.edu
Date: 14 Feb 1995 09:05:10 -0800
Subject: Ecomet Polisher for sale

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Message-ID: {n1419369693.44281-at-maillink.berkeley.edu}

Subject: Time: 9:13 AM
OFFICE MEMO Ecomet Polisher for sale Date: 2/14/95

FOR SALE: ECOMET 1 8" Polisher/grinder, complete with various polishing
discs, alumina and lapping oil. 115 volt, 5 amp, new price in 1988 =
$1250.00
Best offer.
Call Doug Davis of EM Lab at UC Berkeley at (510) 642-2085
or e-mail: doug_davis-at-maillink.berkeley.edu






From: jad1-at-cec.wustl.edu (Joe DeMaro)
Date: Tue, 14 Feb 1995 12:02:43 -0600
Subject: SEM well plates

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Any tips on cutting wells out of 24 well cell culture plates would be
greatly appreciated.

Joe
Joseph A. DeMaro
Washington University Medical School
Department of Neurology
660 S. Euclid
Rm 212 Biotech
St. Louis, MO 63110
jad1-at-cec.wustl.edu
314-362-9448





From: Self :      SALES/GREGB
Date: Thu, 9 Feb 1995 13:25:54
Subject: Re: printers for gray scale prints

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Forwarded message:

All,

LaserMaster Corporation - Imaging Division is the ONLY company
that manufactures a 1800 dpi plain paper laser printers. I work for
LaserMaster and have just completed printing the 100/300 dpi Round
Robin Greyscale Test images. If you are interested in obtaining
them, you may e:mail me at Gregb-at-Sales.LMT.com and I will mail you a
printout of the test images from the LaserMaster printer. I can be
reached at 1-800-950-6363 Ext: 3207 if you have questions about your
specific situation and resolution needs.

Thank You all,
Greg Begin - LaserMaster Corp.
Scientific/Medical Imaging Div.
/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\\/\/\/\/\/\/\/\/\/

} Date sent: Thu, 09 Feb 95 07:42:30 -600
} From: Supratik_Guha-at-mail.mmmg.com (SG)
} To: microscopy-at-aaem.amc.anl.gov
} Subject: printers for gray scale prints

} I am looking around for a gray scale printer to attach with our Gatan
} slow scan CCD image aquisition system and would appreciate any
} suggestions. We would prefer not to get a dye-sub printer due to the
} high costs of printing. I understand that there are 1800 dpi laser
} printers available, could someone point out manufacturers for these
} machines?
}
} Supratik Guha
} Senior Materials Scientist
} 3M Corporate Research Labs.
}




From: NANCY SMITH :      NSMITH-at-darwin.sci.csuhayward.edu
Date: Tue, 14 Feb 1995 13:51:37 PSD8PDT
Subject: sem culture well

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Hello Joe:

When I want to process cells for SEM from 24 well culture dishes I
use a Bunsen burner and a scoopula (the curved metal device for
scooping out dry chemicals). First, the cells are fixed as usual with
glutaraldehyde then washed in buffer and distilled water. Then,
working within a fume hood and wearing a heatproof glove, the scoopula
is heated until red then touched to the underside of the culture dish.
The curved metal is about the right size for the 24 well dishes. It
takes 2 or 3 times to heat and cut until the whole bottom is
released. Once the initial cut is made you need to keep the cells wet
and this is easily done using a squirt bottle of distilled water.
This technique does not appear to cause damage to the cells but we
normally look at the more centrally positioned cells to avoid any
artefacts. Hope this helps.

Nancy Smith
Cal State Hayward
nsmith-at-csuhayward.edu




From: Eric Wang :      ewang-at-u.washington.edu
Date: Tue, 14 Feb 1995 15:07:16 -0800 (PST)
Subject: Electron mean free path

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X-Sender: ewang-at-hardy.u.washington.edu

Hello, everyone:
Does anyone know where I can find some reference on measurement
of electron mean free path of different materials? The mean free path I
mean here is the mean free path for measuring the thickness when doing
EELS, so this includes electrons of all the energy losses rather than one
particular energy loss. We are particularly interested in getting the
right electron mean free path for Chrome Oxide and evaporated Carbon.
Thanks a lot.

Eric Wang
FB-10 Roberts Hall
Univ. of Washington
Seattle. Wa 98195
(206) 543-1514




From: richard.easingwood-at-stonebow.otago.ac.nz (Richard Easingwood)
Date: Wed, 15 Feb 1995 16:49:15 +1100
Subject: SEM well plates

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Message-Id: {199502150442.RAA12340-at-arwen.otago.ac.nz}
X-Sender: st004718-at-brandywine.otago.ac.nz
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

)Joe,
} Subject: SEM well plates

} Any tips on cutting wells out of 24 well cell culture plates would be greatly
} } appreciated.
} Joe
} Joseph A. DeMaro

Depending on what exactly it is you are doing, how about using Thermanox
(Thermonox?) plastic slides on the bottom of the wells - they make them
specially to fit into the wells of 12 well plates and possibly the 24 well
ones too. They come sterilised and you just pop them into the well before
you add medium and cells and remove later, fix, dry etc and mount on a
stub. The slide surface properties are supposed to duplicate the ordinary
well bottoms.
Its easier than cutting wells out of the bottom of the trays.
Regards R Easingwood

Richard Easingwood
South Campus Electron Microscope Unit
Otago Medical School
PO Box 913
Dunedin
NEW ZEALAND

Telephone: 64-03-479 7301
Facsimile: 64-03-479 7254






From: MatlsMicrs-at-aol.com
Date: Wed, 15 Feb 1995 01:11:08 -0500
Subject: Subscribe

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Please Subscribe




From: Rich2442-at-aol.com
Date: Wed, 15 Feb 1995 02:45:32 -0500
Subject: Request to join mailing list

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I work for an OEM of scanning electron microscopes and am interested in
keeping up with the latest technology and issues. I would like to subscribe
to the mailing list. Could you please let me know what I need to do.




From: W.L. Steffens :      STEFFENS.B-at-calc.vet.uga.edu
Date: Wed, 15 Feb 1995 08:41:37 EST
Subject: Cell culture plates

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We process a considerable number of cell cultures for both SEM and TEM,
and have found what we consider to be the ideal system. For this purpose,
we have our investigators culture their cells in Leighton tubes...these
are cell culture tubes with a flat bottom which holds a long, narrow
plastic coverslip. Once the cells are attached, the medium is replaced
with fixative, then the coverslip (which is attached to a nifty little
handle) is removed. The plastic on the coverslip is impervious to all
solvents used in microscopy, and can be embedded and sectioned for TEM.

I wouldn't think of doing it any other way.

-=W.L. Steffens=-
College of Veterinary Medicine
University of Georgia




From: Fermin, Cesar :      Fermin.Cesar-at-tmc.tulane.edu
Date: 15 Feb 1995 09:37:10 -0600
Subject: Beseler Enlarger tune-up

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ref.: Beseler enlarger tune-up.

We have two Beseler enlarger needing adjustments. Any information
on available service person in the south, please remit details
directly to me. Number I called was disconnected!

************************************************************
*Cesar D. Fermin, Ph.D \|*|/ Fax (504) 587-7389 *
*Tulane Medical School /|*|\ Answ. Mach.(504) 584-2618 *
*Pathology/SL79 \|*|/ Secretary (504) 584-2436 *
*New Orleans, La 70 112 /|*|\ Lab (504) 5841 *
*Fermin-at-TMC.Tulane.edu -} Director of Morphological Services*
************************************************************




From: dhoyle-at-tic.ab.ca (David Hoyle)
Date: Wed, 15 Feb 1995 10:59:42 -0700
Subject: registration

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Message-Id: {25021509453900-at-vms2.macc.wisc.edu}

Thank you for responding so quickly to my inquiry.
I look forward to your news letters and hope to be able to
contribute any information I can.

Subscribe Microscopy dhoyle.tic.ab.ca





From: Michael Cammer :      cammer-at-aecom.yu.edu
Date: Wed, 15 Feb 1995 11:24:52 -0500 (EST)
Subject: Re: Beseler Enlarger tune-up

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Back around '82 or '83 I found that I had a problem with my Besseler's
constantly slipping focus just a tiny bit. So I put pipe clamps (those
metal rings that can be tightened of loosened with the turn a a screw) on
the metal guides for the focus which I would tighten when focusing the
bellows particulalry tight.
-mc

On 15 Feb 1995, Fermin, Cesar wrote:
} ref.: Beseler enlarger tune-up.






From: W.L. Steffens :      STEFFENS.B-at-calc.vet.uga.edu
Date: Wed, 15 Feb 1995 13:37:13 EST
Subject: Leighton Tubes

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For those interested, Leighton tubes for cell culture are manufactured by
Corning Costar and are available from Fisher Scientific (Cat.# 07-200-
367). They appear in the 95-96 catalog on page 721.

-=W.L. Steffens=-
College of Veterinary Medicine
University of Georgia




From: tivol-at-tethys.ph.albany.edu
Date: Wed, 15 Feb 1995 15:14:40 EST
Subject: Re: Electron mean free path

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Dear Eric,
If no expert in the field has a table of the mfp's, I can fax you
tables of stopping powers for electrons in carbon, oxygen, iron & titanium--
you have to interpolate for chromium and calculate the mpf from dE/dx. Good
luck.
Yours,
Bill Tivol




From: BARBARA.HARTMAN-at-1773.220.SCHERING-PLOUGH.sprint.com
Date: Thu, 16 Feb 1995 08:42:49 -0500
Subject: FIXATION OF TESTES FOR HISTOLOGY

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GREETINGS,

DOES ANYONE KNOW OF A BETTER FIXATIVE FOR TESTES THAN BOUINS FOR
LIGHT MICROSCOPY? WE ARE TRYING TO AVOID THE LONG RINSING REQUIRED WITH
BOUINS.

THANK YOU!

BARBARA HARTMAN
SCHERING-PLOUGH RESEARCH
LAFAYETTE, NJ
(201) 579-4343
(201) 570-4211 (FAX)

E-MAIL:
MAIL/ADMD=TELEMAIL/PRMD=SCHERING-PLOUGH/PN=BARBARA.HARTMAN/C=US/-at-SPRINT.COM






From: SiSTek-at-aol.com
Date: Thu, 16 Feb 1995 13:48:59 -0500
Subject: Need TEM / Many thanks!!

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Many thanks to all who responded for my call for help with locating TEM
service near us in the Phoenix metro area. As we thought, there is an
established group in Phoenix who have been around for a few years and who
provide TEM services.

For anyone else who might be interested, the company is called NanoTEM, Inc,
7620 E. McKellips Rd., Suite 4109, Scottsdale, Arizona 85257, phone 602 759
2808, fax 602 947 7615.

Again, many thanks for all your help.

Mark Anderson, SiSTek




From: baskin-at-biosci.mbp.missouri.edu (Tobias Baskin)
Date: Fri, 17 Feb 1995 13:47:11 -0600
Subject: TEM/formvar substitute/thin films

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Greetings,
Is there something out there that will make a thin film that
isn't formvar? I have been using wire loops coated with a film of 1.2%
formvar to support my small samples during rapid freezing and substitution.
This works fine for acetone substitution but we would like to try
Tetrahydrafuran (THF) as a substitution medium and, alas, THF eats the
formvar.

We get a formvar film on the wire loop by casting small rectangles
of formvar on water and then trasfering one to a loop.

Are there other compounds that could be used to make a film across
the loop and that might survive THF??

Thanks for any suggestions,

Tobias Baskin

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
___ ____ ^ ____ _____ Tobias I. Baskin
/ \ / / \ / \ / University of Missouri
/ | / / \ / / Biological Sciences
/___ / /__ /_____\ / /__ 109 Tucker Hall
/ / / \ ( / Columbia, MO 65211 USA
/ / / \ \ / voice: 314-882-0173
/ /____ / \ \____/ /_____ fax: 314-882-0123






From: Liang, Long :      LLIANG-at-is.Arco.COM
Date: 17 Feb 1995 14:34:14 CST
Subject: Sample Prep--Steel

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Message-Id: {MACMS.LLIANG.644426140095048FMACMS-at-IS.ARCO.COM}


Dear Microscopists,

I am trying to prepare polished sections from steel samples for EPMA
analysis. Are there any recommended abrasive/size for rough grinding,
fine grinding, rough polishing, and final polishing?

Your help is high appreciated.

Long Liang
ARCO EPMA/SEM Lab
Plano, TX
LLIANG-at-is.arco.com






From: Doug Arrell :      ARRELL-at-jrc.nl
Date: Mon, 20 Feb 1995 08:25:04 GMT+0200
Subject: Re: Sample Prep--Steel

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Message-Id: {MAILQUEUE-101.950220082504.256-at-FS-IAM-1.JRC.NL}

}
} I am trying to prepare polished sections from steel samples for EPMA
} analysis. Are there any recommended abrasive/size for rough grinding,
} fine grinding, rough polishing, and final polishing?

I have always stuck to the simple silicon carbide paper (in steps
from 120 to 1200 grade) and then diamond (6,3,1um) route, and found
no problems with that.

Doug

+------------------------------------+
| Dr Douglas Arrell |
| Mechanical Performance and Joining |
| Institute for Advanced Materials |
| 1755 ZG Petten |
| Netherlands |
| {ARRELL-at-JRC.NL} |
| Tel. (+31) 2246 5287 |
| Fax (+31) 2246 1917 |
+------------------------------------+




From: Joyce Craig :      bafpjec-at-uxa.ecn.bgu.edu
Date: Mon, 20 Feb 1995 11:10:51 -0600 (CST)
Subject: student microscopy competition

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CALL FOR PAPERS
STUDENT COMPETITION
TO BE HELD AT
THE UNIVERSITY OF WISCONSIN
WHITEWATER, WISCONSIN
Friday, March 24, 1994
AWARDS:
FIRST PLACE $100
SECOND PLACE $75
THIRD PLACE $25
Abstracts will be published in Midwest Microscopy.
Microsgraphs from first place winner will be on the cover of Midwest
Microscopy.
Students will be judged on written abstract, presentation, and quality of
study.
Student competition is open to undergraduate and graduate students and
may involve any type of microscopy.
Student and sponsoring faculty member must be members of MSEM.
Abstracts should be submitted before March 15 to :
Dr. Lance Urven
University of Wisconsin- Whitewater
800 West Main,Whitewater, WI 53190





From: John Mansfield :      John_Mansfield-at-mse.engin.umich.edu
Date: 20 Feb 1995 12:02:20 -0400
Subject: TEM: Metals,Electropolishing

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Message-ID: {n1418836731.26217-at-mse.engin.umich.edu}

John Mansfield
North Campus Electron Microbeam Analysis Laboratory
413 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (313)936-3352 FAX (313)936-3352
jfmjfm-at-engin.umich.edu or John.F.Mansfield-at-umich.edu Time:
11:59 AM

Date:2/20/95
URL: http://www.engin.umich.edu/~jfmjfm/jfmjfm.html NC EMAL

I have some colleagues that want to electropolish some fairly heavily
deformed steel. Composition is:
0.4% C
0.6-0.9%Si
22-24%Cr
7-9%Ni
Trace N
Balance Fe.
Does anyone have a good starting solution and condidtions for this alloy?
Many Thanks.
John Mansfield.





From: tayloe-at-rorc.usbm.gov
Date: Mon, 20 Feb 1995 13:23:06 -0600 (CST)
Subject: Re: Sample Prep--Steel

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} I am trying to prepare polished sections from steel samples for EPMA
} analysis. Are there any recommended abrasive/size for rough grinding,
} fine grinding, rough polishing, and final polishing?

A step that may be advantageous is the final polish of the steel by
use of a colloidal silica type solution [0.05 micron, with 9.8pH].
Dampen the cloth [such as a Buehler Mastertex] with distilled water;
apply liberal amount of the solution [such as Buehler Mastermet] to the
cloth, and apply firm pressure to soft pressure over a period of ~45
seconds, rotating the sample quite abit. A final word of caution:
this solution [Mastermet], besides having the high pH [rough on skin],
will crystallize into small, -very hard- particles. Is therefore highly
advised to filter the solution a few times into a smaller bottle before
each use. Have found this stuff to be -very- effective tho'. LECO also
has a similar solution, but have not used it enough to get comfortable
with it as the Mastermet.

In the previous steps I have used the 120 to 800 grit SiC papers, then
a 9, 6, 1, & sometimes 1/4 micron diamond paste, on Texmet for the
rough polish (9 & 6), and then Mastertex for the 1/4u. All depends on
the grade and condition of the steel tho'... [all these recommendations
are based on me hand polishing individual samples, and 3-6 samples in a
ring held in hand]

Hope this helps,
-Rob
PS: I have no ties with Buehler, just a satisfied customer for +10 years.
,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
| Rob Tayloe | MSM Spelunkers Club /\v/\ |
| Metallographic Lab. | Missouri Speleological Survey /\v/\ |
| Rolla Research Center | Bat Conservation International /\v/\ |
| U.S. Bureau of Mines | Missouri Cave & Karst Conservancy |
| tayloe-at-rorc.usbm.gov | National Speleological Society #32993 /\v/\ |
| (314) 364-3169 x247 | American Cave Conservation Association |
''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''




From: South Bay Technology :      73531.1344-at-compuserve.com
Date: 20 Feb 95 16:15:25 EST
Subject: Polishing Steel/Colloidal Silica

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The techniques described by Rob Tayloe are certainly in agreement with the
references I have for polishing steel and will work quite well. The only note
I would make is that we do supply a Colloidal Silica which DOES NOT CRYSTALLIZE.
This is an important feature as Rob has mentioned because the crystallized
particles can be a real problem if you do not realize they are there.

Our Non-Crystallizing Colloidal Silica is available as follows:

Part No. Description Price
CS1-16 Non-Crystallizing Colloidal Silica 16 oz bottle $16.00
CS1-128 " " " 1 gallon bottle 78.00

If you'd like more information (MSDS etc) on this prodcut or any of our other
products, please let me know.

Best regards-

David Henriks
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673 USA

TEL: 800-728-2233
714-492-2600
FAX: 714-492-1499





From: sbarlow-at-sunstroke.sdsu.edu (Steve Barlow)
Date: Mon, 20 Feb 1995 15:01:32 -0800
Subject: Tem: insect heart

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one of my users is looking at TEM of insect heart. Does anyone have some good
references on insect heart ultrastructure? Some of the cells associated with
the heart appear highly vesiculated at the cell periphery. The morphology of
these cells looks good, so we don't feel these stuctures are an artifact, but
we have so far been unable to identify what kind of cell or cell type it might
be. Can anyone suggest a reference or an investigator we could contact in
this regard?

----------------------------------------------------------
Dr. Steven Barlow
EM Facility/Biology Dept.
San Diego State University
San Diego CA 92182-0057
phone: (619) 594-4523
fax: (619) 594-5676
email to sbarlow-at-sunstroke.sdsu.edu





From: SUSAN R. SESACK :      SESACK-at-brain.bns.pitt.edu
Date: Tue, 21 Feb 1995 08:45:41 EDT
Subject: enrollment

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Message-Id: {25022013111522-at-vms2.macc.wisc.edu}

Dear Colleagues,

Would someone please provide me with instructions for enrolling in
the Internet bulletin board for histology and microscopy? Much
obliged.

S.




From: R_HOLLAND_CHENG :      RHC-at-justem.bio.purdue.edu
Date: Tue, 21 Feb 1995 9:41:45 -0500 (EST)
Subject: RE: enrollment

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} SUSAN R. SESACK wrote:
}
} Would someone please provide me with instructions for enrolling in
} the Internet bulletin board for histology and microscopy? Much
} obliged.


To subscribe, please send a mail to "Listserver-at-AAEM.AMC.ANL.GOV"
with "Subscribe Microscopy your_id-at-e-mail" in the body. Enjoy it.

Holland Cheng
--------------------
Structural Biology
Department of Biological Sciences
Purdue University
W. Lafayette, IN 47907-1101

InterNet: rhc-at-bragg.bio.purdue.edu




From: R_HOLLAND_CHENG :      RHC-at-justem.bio.purdue.edu
Date: Tue, 21 Feb 1995 10:03:59 -0500 (EST)
Subject: RE: enrollment

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} SUSAN R. SESACK wrote:
}
} Would someone please provide me with instructions for enrolling in
} the Internet bulletin board for histology and microscopy? Much
} obliged.


To subscribe, please send a mail to "Listserver-at-AAEM.AMC.ANL.GOV"
with "Subscribe Microscopy your_id-at-e-mail" in the body. Enjoy it.


Btw, can someone in the server fix the returning route so that
the reply can a global one? I would like to be on a list that
I can see discussions (questions and answers) rather than a
collection of of questions. Thanks in advance!


Holland Cheng
--------------------
Structural Biology
Department of Biological Sciences
Purdue University
W. Lafayette, IN 47907-1101

InterNet: rhc-at-bragg.bio.purdue.edu




From: cel1-at-Lehigh.EDU (Charles Lyman)
Date: Tue, 21 Feb 1995 11:44:22 -0500
Subject: Preparing polished sections of steel samples for EPMA analysis

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++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
++++I would like to add a small point about polishing specimens for
analysis in the electron probe microanalyzer (EPMA). I prefer to leave the
scratches in from the 1/4micron diamond polishing step in order to have an
image feature on which to focus with the light optics. Since quantitative
x-ray microanalysis samples should be flat-polished but unetched, it is
hard to find a suitable surface feature to use for focusing without these
fine scratches. For some reading on this, try Chapter 11 of Goldstein et
al., Scanning Electron Microscopy and X-ray Microanalysis, 2nd edition,
Plenum Press, 1992.

Good luck, Prof. C E Lyman

Electron Optics Lab
Lehigh University
5 East Packer Avenue
Bethlehem, PA 18015
++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++

} } I am trying to prepare polished sections from steel samples for EPMA
} } analysis. Are there any recommended abrasive/size for rough grinding,
} } fine grinding, rough polishing, and final polishing?
}
} A step that may be advantageous is the final polish of the steel by
} use of a colloidal silica type solution [0.05 micron, with 9.8pH].
} Dampen the cloth [such as a Buehler Mastertex] with distilled water;
} apply liberal amount of the solution [such as Buehler Mastermet] to the
} cloth, and apply firm pressure to soft pressure over a period of ~45
} seconds, rotating the sample quite abit. A final word of caution:
} this solution [Mastermet], besides having the high pH [rough on skin],
} will crystallize into small, -very hard- particles. Is therefore highly
} advised to filter the solution a few times into a smaller bottle before
} each use. Have found this stuff to be -very- effective tho'. LECO also
} has a similar solution, but have not used it enough to get comfortable
} with it as the Mastermet.
}
} In the previous steps I have used the 120 to 800 grit SiC papers, then
} a 9, 6, 1, & sometimes 1/4 micron diamond paste, on Texmet for the
} rough polish (9 & 6), and then Mastertex for the 1/4u. All depends on
} the grade and condition of the steel tho'... [all these recommendations
} are based on me hand polishing individual samples, and 3-6 samples in a
} ring held in hand]






From: tivol-at-tethys.ph.albany.edu
Date: Tue, 21 Feb 1995 13:19:07 EST
Subject: Re: TEM/formvar substitute/thin films

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Dear Tobias,
A carbon film--evaporated onto freshly-cleaved mica--should do the
trick. After evaporation, float the film onto water and pick it up with the
loop. You may have to experiment with thickness etc. to get the proper mech-
anical strength, but it should certainly survive the THF. You may also want
to try a mesh grid instead of the loop if the carbon film is not strong enough.
Good luck.
Yours,
Bill Tivol




From: {tivol-at-tethys.ph.albany.edu}:ddn:wpafb
Date: 2-21-95 1:51pm
Subject: Re: TEM/formvar substitute/thin films

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Message-Id: {9502212303.AA26486-at-riker.ml.wpafb.af.mil}
To: WALCKSD:ml:wpafb, SCHELTFJ:ml:wpafb
Subj: Re: TEM/formvar substitute/thin films
Orig-Author: {tivol-at-tethys.ph.albany.edu}:ddn:wpafb
-----------------------------------------------------------
Dear Tobias,
A carbon film--evaporated onto freshly-cleaved mica--should do the
trick. After evaporation, float the film onto water and pick it up with the
loop. You may have to experiment with thickness etc. to get the proper mech-
anical strength, but it should certainly survive the THF. You may also want
to try a mesh grid instead of the loop if the carbon film is not strong enough.
Good luck.
Yours,
Bill Tiv





From: Dave DeFily :      defily-at-tam2000.tamu.edu
Date: Wed, 22 Feb 1995 13:41:26 -0600
Subject: graphs to data

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Matthias -

I've done this quickly with "Image" by measuring the distance from the graph
axis (x or y) to the data points in question. To calibrate (pixels to data
units), just measure the distance between axis values.

-Dave defily-at-tamu.edu





From: Elinor Solit :      cambrex-at-world.std.com
Date: Wed, 22 Feb 1995 15:45:00 +0001 (EST)
Subject: Re: New SEM any Suggestions??

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Mark,

We published an article last Fall on the criteria and process for
purchasing an SEM. Readers tell us it is useful.

Suggest you or your friend call for more information.

Ellie Solit,
Executive Editor, Microscope Technology & News.
800-440-0311


On Wed, 22 H
Feb 1995 Noonan_Eddie/perth-at-perth.atd.cra.com.au wrote:

} I have been asked by a colleague of mine who at present does not have
} access to the Microscopy Listserver and who presently is sourcing a
} new SEM for his lab to put out the following question.
}
} "In the next few weeks I shall be ordering a new analytical SEM. We
} will use this SEM almost exclusively for EDS analysis. A motorised
} stage will also be required for the SEM to accommodate overnight runs.
} Therefore I require an ultra stable, reliable instrument. If any one
} with experience in this area has any thoughts I would be grateful to
} hear from you."
}
} Thanks in advance
}
} Mark Stewart
}
} Replies to: ejn-at-perth.atd.cra.com.au
}




From: m.dickson-at-unsw.edu.au (Melvyn R. Dickson)
Date: Thu, 23 Feb 1995 12:24:26
Subject: Re: Fixation of blood cells

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To: microscopy-at-aaem.amc.anl.gov






From: m.dickson-at-unsw.edu.au (Melvyn R. Dickson)
Date: Thu, 23 Feb 1995 12:29:17
Subject: Re: Blood fixation

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To: microscopy-at-AAEM.AMC.ANL.GOV

Jan Coetzee and Philip Oshel have posted about blood fixation but I havn't
seen any replies on this topic. We have a project that will try to tie
distortion of red cells to heat stress so fixation needs to be as artefact
free as we can get it. Please Jan or Philip can you mail me with the best
method you can recommend?

Mel Dickson, Univ. of NSW

m.dickson-at-unsw.edu.au




From: Glenn Holm :      KARUZIS-at-wccf.mit.edu
Date: Thu, 23 Feb 1995 00:27:16 -0500 (EST)
Subject: 3 questions

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3 separate requests for information:

1) In the February '95 Biotechniques, New Products section, there's
a blurb for a "Probe Clip" single slide incubation chamber sold by
Grace Bio-Labs. Anyone have experience with these and/or a contact
phone/fax # for Grace Bio-Labs?

2) A grad student in our lab has been doing ImmunoGold Silver (LM)
immunostaining followed by BrDU - HRP - DAB for a second antigen.
The silver was originally present, but faded out in the second reaction.
Could have resulted from a number of factors, but what we were wondering
is - is it possible to stabilize the silver with a sodium thiosulfate
"fixer" step after the IGSS to protect it in subsequent immunoreactions,
dehydrating and coverslipping? Any practical suggestions would be appre-
ciated.

3) A colleague in Australia wants to purchase an antiserum to met-
enkephalin. We have been buying from Incstar and getting good results,
but the Australian distributor for Incstar charges outrageous prices.
Does anyone know of an alternate antibody supplier that may be less ex-
pensive for Australian customers?

------------------------------------------------------------------
|Glenn Holm *mime mail ok* Internet:karuzis-at-wccf.mit.edu |
|M.I.T Dept. of Brain + Cog. Sci. This VAX doesn't do NeXTmail |
|Cambridge, MA 02139 "Real Neuroscientists don't do gels!" |
------------------------------------------------------------------





From: KAKER-at-ctklj.ctk.si
Date: Thu, 23 Feb 1995 8:13:54 +0100 (WET)
Subject: Coster-Kronig

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Dear Microscopists,

I am looking for Coster-Kronig transition probabilities for M lines.

Henrik Kaker
SEM/EDS Lab
Metal d.o.o.
62390 Ravne
Slovenia

Kaker-at-Ctklj.ctk.si




From: Kathy Walters :      kwalters-at-emiris.iaf.uiowa.edu
Date: Thu, 23 Feb 1995 08:20:45 -0600 (CST)
Subject: lipid sizing

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Recently I posted a request for methods for lipid sizing. I have put
together a summary of that request. If anyone would like a copy I will be
happy to send you one, but it is rather lengthy for the listserver.

Thanks to all respondents,

Kathy Walters






From: Marc Brande :      brande-at-sdsc.edu
Date: Thu, 23 Feb 1995 09:25:49 -0800 (PST)
Subject: Re: 3 questions

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ProbeClip

GBL inc. 1-800-813-7339 810-332-7100

Marc C. Brande, M.S. SD3D Email List:3D Imaging
San Diego 3D Imaging Group To subscribe/unsubscribe:listserv-at-bobcat.etsu.edu
3840 Camino Lindo To post a message:sd3d-at-bobcat.etsu.edu
San Diego, CA 92122 Email:BRANDE-at-SDSC.EDU Voice:619-587-4830


On Thu, 23 Feb 1995, Glenn Holm wrote:

} 3 separate requests for information:
}
} 1) In the February '95 Biotechniques, New Products section, there's
} a blurb for a "Probe Clip" single slide incubation chamber sold by
} Grace Bio-Labs. Anyone have experience with these and/or a contact
} phone/fax # for Grace Bio-Labs?
}
} 2) A grad student in our lab has been doing ImmunoGold Silver (LM)
} immunostaining followed by BrDU - HRP - DAB for a second antigen.
} The silver was originally present, but faded out in the second reaction.
} Could have resulted from a number of factors, but what we were wondering
} is - is it possible to stabilize the silver with a sodium thiosulfate
} "fixer" step after the IGSS to protect it in subsequent immunoreactions,
} dehydrating and coverslipping? Any practical suggestions would be appre-
} ciated.
}
} 3) A colleague in Australia wants to purchase an antiserum to met-
} enkephalin. We have been buying from Incstar and getting good results,
} but the Australian distributor for Incstar charges outrageous prices.
} Does anyone know of an alternate antibody supplier that may be less ex-
} pensive for Australian customers?
}
} ------------------------------------------------------------------
} |Glenn Holm *mime mail ok* Internet:karuzis-at-wccf.mit.edu |
} |M.I.T Dept. of Brain + Cog. Sci. This VAX doesn't do NeXTmail |
} |Cambridge, MA 02139 "Real Neuroscientists don't do gels!" |
} ------------------------------------------------------------------
}






From: Dave L Robinson :      robin019-at-maroon.tc.umn.edu
Date: Thu, 23 Feb 1995 13:20:49 -0600 (CST)
Subject: How do I subscribe?

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How do I subscribe to this newsgroup?

Thank you!

Dave Robinson
University of Minnesota

robin019-at-maroon.tc.umn.edu




From: nee-at-beta.lanl.gov (Norman Elliott)
Date: Thu, 23 Feb 1995 14:09:10 -0700
Subject: TEM negatives

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Dear list,

I believe this question was asked on this list before, so I am
sorry if I am repeating. A colleague here is having problems with
negatives being ruined by static discharge in a JEOL 2010 TEM. JEOL
suggests drying the negatives inside the TEM vacuum which works but is
really not good and very inefficient. The problem began only recently when
atmospheric humidity is increasing here. Does anyone know the cause of the
static discharge problem and/or have a practical solution.




Norman Elliott | E-mail: nee-at-lanl.gov
Los Alamos National Lab | Fax: 505-665-2104
MST-7 MS E549 | Voice: 505-667-1587
Los Alamos, NM 87545 |






From: KJMcCarthy :      KJMCCARTHY-at-bmg.bhs.uab.edu
Date: Thu, 23 Feb 1995 15:08:07 CST+6CDT
Subject: Digital Imaging Microscopy/FTP site

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Message-ID: {MAILQUEUE-101.950223140219.480-at-bmg.bhs.uab.edu}

I would like to announce the establishment of an FTP site for the Macintosh/Power PC
based digital imaging microscopy software IPLabspectrum from Signal Analytics
Corporation. There are several reasons for development of this site:
1. To provide individuals interested in developing a digital imaging microscopy system
a chance to access and download demonstration versions of this particular
Macintosh/PowerPC based digital imaging software for evaluation purposes.

2. To provide users of IPLabspectrum software for a site to download and upload
user-generated system extensions and scripts .

Although the software is from a commercial source, the establishment of the FTP site
was a decision by made by myself and other users of the Digital Imaging Microscopy
Facility here at the University of Alabama at Birmingham as one way of promoting the
digital imaging microscopy as a research tool. Signal Analytics Corporation, the
developers of the software, has generously made available demonstration versions of their
entire software line for FTP download purposes. If any other developers of imaging
software wish to use this site as a repository for demonstration versions of their software I
would be more than happy to accomodate them.

The demonstration versions of this software are organized into several folders, and
separated into Macintosh and PowerPC versions of that software. There are versions of the
software that support specific imaging boards (e.g. Scion LG-3) in the demo folders. Also in
the site are demo versions of calcium ratio (IPLab Ratio), Three Dimension Reconstruction,
and Multiprobe (FISH) extensions. In addition is a separate program for reading scanned
images of electrophoretic gels. (IP Lab Gel)

There is a folder (directory) for Uploading of Scripts, Extensions, Messages, ect.
provided-however the caveat there is that downloading the extensions from that
directory is at your own risk, since this is a public access directory. If you choose to
download from this directory, please take the time to scan the files for viruses or other
nasties. Our aim is to monitor the upload directory as the usage increases, test the material
in the directory for problems (bugs, viruses, and other annoying creatures) and then shift
those programs that we consider problem free to a specific download-only directory. At
present this download-only directory for user-uploaded files does not exist.

To access the site use the address FTP.BMG.UAB.EDU, logon as ANONYMOUS to enter the
public directory. In the directory is a subdirectory (folder) IP_Labspectrum. Within this
subdirectory are other directories containing the various demos of the software. I also
included the shareware programs FETCH and BinHex 5.0 in a directory named
FTP_SITE_Utilities for individuals (like myself) who rather use FETCH as a front end for
FTP browsing and transfer.

This site is still undergoing development, so please forgive the style of organization. Any
comments, suggestions, ect. about the site, digital imaging microscopy would be greatly
appreciated. My e-mail address is KJMcCarthy-at-CellBio.BMG.UAB.EDU

Best Regards
Kevin McCarthy
Assistant Professor
Department of Cell Biology
University of Alabama at Birmingham
Birmingham, Alabama 35294
Phone 205-934-9923/9924
Fax 205-934-7029




From: Leo D Frawley 03 5667464 :      FRAWLEY-at-a1.resmel.bhp.com.au
Date: Fri, 24 Feb 1995 11:44:58 +1100
Subject: Extraction Replicas MnS ppts.

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Return-Receipt-To: FRAWLEY-at-a1.resmel.bhp.com.au
Registered-Mail-Reply-Requested-By: FRAWLEY-at-a1.resmel.bhp.com.au
Mr-Received: by mta VULCAN.MUAS; Relayed; Fri, 24 Feb 1995 11:44:58 +1100
Mr-Received: by mta VULCAN; Relayed; Fri, 24 Feb 1995 11:59:09 +1100
Disclose-Recipients: prohibited

I am interested in using an extraction replica technique to determine MnS precipitate size distributions in a
TEM.

The technique being considered is as follows:
-Polish sample
-Etch to give some surface relief
-Carbon coat
-Using blade cut 1mm size grid on surface
-Release carbon film containing ppt's. from surface using etchant
-Wash carbon replica in alcohol and water
-Position on TEM grid.

My problem is finding an etchant that will not attack the very small MnS ppts. It has been reported that some
etchants attack the Mn in these ppt's.

Any suggestions on the technique or the etchant would be appreciated.

Regards Leo D Frawley
frawley-at-a1.resmel.bhp.com.au







From: peter smith :      PS-at-bunyip.ph.rmit.edu.au
Date: Fri, 24 Feb 1995 16:20:57 EST-10
Subject: Re: TEM negatives

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} Date sent: Thu, 23 Feb 1995 14:09:10 -0700
} To: microscopy-at-aaem.amc.anl.gov
} From: nee-at-beta.lanl.gov (Norman Elliott)
} Subject: TEM negatives

} Dear list,
}
} I believe this question was asked on this list before, so I am
} sorry if I am repeating. A colleague here is having problems with
} negatives being ruined by static discharge in a JEOL 2010 TEM. JEOL
} suggests drying the negatives inside the TEM vacuum which works but is
} really not good and very inefficient. The problem began only recently when
} atmospheric humidity is increasing here. Does anyone know the cause of the
} static discharge problem and/or have a practical solution.
}
}
We had this static discharge problem when first we bought our
2010. It was solved when JEOL replaced the Teflon drive gear (large)
in the camera with a conducting (metal) one. Coating the gear with a
conducting spray didn't work!
We also installed a separate vacuum desiccator for the plates
(diffusion pump and liquid nitrogen trap).

Peter Smith ps-at-bunyip.ph.rmit.edu.au
RMIT App Physics Ph: (03) 660 3390 Office
GPO Box 2476V (03) 660 2205 Lab
Melbourne Fax: (03) 660 3837
Vic 3001
AUSTRALIA







From: NICK SCHRYVERS :      nick_schryvers-at-ruca.ua.ac.be
Date: 24 Feb 1995 10:27:10 +0100
Subject: Re: TEM negatives

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Message-Id: {n1418500529.67061-at-ematserv.ruca.ua.ac.be}

REGARDING Re} TEM negatives

About your discharge problem, we believe this is indeed strongly linked with
the vacuum. Therefore we always use one or more desicators before the plates
enter the microscope. This way also the vacuum in the microscope restores
faster. The problem also depends on the plates: we had the experience that
Ilford plates always gave discharge, while Kodak and Agfa did not in the same
environment. Also handling of the plates can be important: sliding plates
over one another can cause discharges before and after use in the microscope.
Hope this can be of help,
Nick Schryvers





From: Larry Hawkey :      hawkey-at-neuro.duke.edu
Date: Fri, 24 Feb 1995 08:50:07 -0500
Subject: RE>EM negatives

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I had some static discharge problems with my JEOL 1200exII
some time ago. Every 8th to 10th neg had streaks on
it. they were thin and sharp with two or three finger
like projections coming fron the same point. The service
tech. cleaned the camera (several times) and that cleared the
problem up. It did take several tries and return trips to
get the dirt (thought to be a metal fragment) cleaned up.

I hope this helps.
Larry Hawkey
hawkey-at-neuro.duke.edu
919-641-6425




From: DCROMEY-at-CCIT.ARIZONA.EDU
Date: Fri, 24 Feb 1995 07:48:30 -0700 (MST)
Subject: Re: TEM negatives

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Norman,
Although I am not familiar w/ this particular model of JEOL, could it be
possible that the static discharge is occuring before or (more likely)
after the film has gone into/come out of the scope? We had an individual
in the lab I was in before who tended to wear synthetic fabrics and on
particularly dry Arizona days they could really ZAP the film. One
solution is to wear a wrist grounding strap when handling the film (like
the kind that people are supposed to wear when working inside their
computers). Hope this helps.
Doug


Douglas W. Cromey, M.S.
Cell Biology and Anatomy
Arizona Health Sciences Center
1501 N. Campbell Ave.
Tucson, AZ 85724
(602)626-2824 dcromey-at-ccit.arizona.edu













From: :      Image-at-beelzebub.ucsf.EDU
Date: Thu, 23 Feb 1995 22:36:29 -0800 (PST)
Subject: Re: TEM negatives

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subscribe







From: tivol-at-tethys.ph.albany.edu
Date: Fri, 24 Feb 1995 10:28:53 EST
Subject: Re: TEM negatives

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Dear Norman,
We, too, have had occasional problems with static discharges on our
films--especially with LoDose, which is very sensitive to light. Our worst
problems occur when the humidity is low. You must be very careful when sepa-
rating films from the stack and when removing the stack from the package. You
might also check if your lab coats produce static electricity--we just got new
polyester coats which are terrific generators. If you are completely dark-ad-
apted and loading the folm in total darkness, you can see the discharges as
they occur, so at least you will know what is going on. [oops, I meant film]
If your desiccant is too efficient, the discharge problem will be worse. Good
luck, sometimes the procedures necessary to prevent these discharges are te-
dious. If all else fails, try using 4489 film--it's not nearly as sensitive
as SO163, but it is much finer grain and gives excellent images. We've never
had discharge problems with it, probably because discharges are not intense
enough to show up.
Yours,
Bill Tivol




From: dlmedli-at-california.sandia.gov (medlin douglas l)
Date: Fri, 24 Feb 1995 09:11:50 -0800
Subject: Re: em negatives

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In the past we too have had problems with static discharge ruining negatives
(the film was SO163 being used in a JEOL 4000EX). We found that
the discharges were coming from our vinyl gloves; when we switched to
cotton gloves the problem went away. Additionally, when loading
the camera we never use pre-dessicated film, but instead load the
camera with film that has been at atmosphere (and warmed up to room
temperature if it's been in the refrigerator). We then dessicate the camera
box prior to putting it in the microscope.

On a related topic, on occasion we've observed a fine network of cracks in
the emulsion of our SO163 film. Under an optical microscope the film
looks similar to a dried out mud flat with cracks spaced rougly
0.05 mm apart. These cracks would appear regardless of whether
we dried the film at room temperature overnight or in a heated film drier.
The cracking also appeared with both new and old chemicals (D19 diluted
2:1 4 minutes; kodak rapid fixer 5 minutes). Thinking we had a bad
batch of film we sent some of the material to Kodak, but they were
unable to duplicate the effect. Finally, it was suggested that we
reduce the concentration of hardener in the fixer to half of what
Kodak recommends. This seems to have reduced the cracking quite a bit, although
not entirely. Has anyone had similar problems?

+---------------------------------------------+
! Douglas L. Medlin !
! Physical Properties of Materials Department !
! Mail Stop 9402 !
! Sandia National Laboratories !
! Livermore, California 94551 !
! !
! (510) 294-2825 !
! dlmedli-at-california.sandia.gov !
+---------------------------------------------+
.\




From: Glenn Poirier :      GLENN_P-at-GEOSCI.Lan.McGill.CA
Date: Fri, 24 Feb 1995 13:51:56 EST5EDT
Subject: anhydrous polishing

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Message-Id: {199502241853.NAA28700-at-sifon.CC.McGill.CA}

Greetings Microscopists

Is there anyone out there who has any tips/techniques on polishing hygroscopic
material for microanalysis. Specifically, I need to polish some CaO
particles with CaC rinds. I could probably polish them with silicone
oil, but how do I get the oil off the samples before I put them in
the microprobe? Any comments or suggestions would be appreciated.

Thanks in advance

Glenn
**********************************************************************
* Glenn Poirier glenn_p-at-geosci.lan.mcgill.ca *
* Electron Microprobe Lab Phone: (514) 398 6774 *
* Earth and Planetary Sciences Fax: (514) 398 4680 *
* McGill University THERE ARE THREE SIDES TO EVERY STORY; *
* Montreal, Quebec YOUR SIDE MY SIDE AND THE TRUTH *
**********************************************************************




From: Po-Fu Huang :      huang020-at-maroon.tc.umn.edu
Date: Fri, 24 Feb 1995 13:14:06 -0600 (CST)
Subject: literature needed

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Greetings,

I am a graduate student currently working on a project utilizing EDS to
get quantitative elemental information of atmospheric particles. In past
few monthes, I have been trying to get hold of a article cited below through
the interlibrary service and without any success. It will be greatly
appreciated if anyone out there can direct me where and how to get this
article.

Heinrich, K. F. J. (1987) "Mass Absorption Coefficients for Electron Probe
Microanalysis." in
"Proc. 11th Int. Cong. on X-ray Optics and Microanalysis." edited by J.
Brown and R. Packwood, published by J. D. Brown, University of Western,
Ontario, pp67-119.


Po-Fu Huang
Particle Technology Laboratory
Department of Mechanical Engineering
University of Minnesota
(Office) (612) 626-7227
(Lab) (612) 625-7307
(Fax) (612) 625-6069





From: hukee.margaret-at-mayo.EDU (Marge Hukee)
Date: Fri, 24 Feb 1995 14:10:12 +0200
Subject: Re: TEM negatives

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Message-Id: {9502242007.AA12178-at-fermat.Mayo.EDU}
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Content-Type: text/plain; charset="us-ascii"

We pre-pump negatives in a separate vacuum chamber with phosphorus
pentoxide powder placed in the bottom of chamber. This film is loaded into
a spare cassette and has always been suitable for use after being pumped
for 1-2 hours or left under vacuum. After about a week, the powder absorbs
moisture and must be replaced. The old powder can be neutralized with NaOH
pellets and when NaOH pellets have dissolved, can be discarded by flushing
with water.

Marge Hukee
EM Core Facility hukee.margaret-at-mayo.edu
Mayo Foundation (507) 284-3148
----------------------------------------------------------------------------
--






From: John F. Conroy :      John.Conroy-at-m.cc.utah.edu
Date: Fri, 24 Feb 1995 14:23:58 -0700 (MST)
Subject: Optical Microscopy of Tissues

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Hello,

I am currently writing a grant proposal, and I have been knocking my head
against the wall looking for some sort of data on the optical transmission
properties of nervous tissue (the brain). Does anyone have a good
reference, or, from a practical standpoint, how thick do brain tissue
slices have to be to obtain good optical transmission (esp. relative to
other fatty tissue slices like those from the earlobe, whose transmission
characteristics I can find since people have been doing pulse
oximetry.).

Thanks in advance,

John Conroy
University of Utah
Dept. Bioengineering




From: YANGA-at-NCCCOT.AGR.CA
Date: 24 Feb 1995 17:10:04 -0500 (EST)
Subject: Re: EM negatives

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We had some static discharge on our 35 mm film in the past. It
occurred when pre-desiccated bulk was being rolled onto
cassettes. The problem disappeared after bulk film was left in
atmosphere. We never had problem with plates because we did not
pre-desiccate plates. One may try leaving the exposed plates in a
humid chamber for a while before developing them, if the problem
persisted.

Ann Fook Yang




From: emlab-at-ucsco.ucsc.edu (Jon Krupp)
Date: Fri, 24 Feb 1995 14:28:17 -0800
Subject: LKB Ultratome III

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Greetings:

I have an LKB Ultratome III that is surplus to our needs. Purchased in
1978, almost never used. If you might be interested in making an offer or
know of a worthwhile place for a donation, please let me know.

Thanks,


Jonathan Krupp
Microscopy and Imaging Lab
University of California
Santa Cruz, CA 95060
emlab-at-ucsco.ucsc.edu
(408) 459-2477






From: Maggy Piranian :      maggy-at-sparky2.esd.mun.ca
Date: Fri, 24 Feb 1995 17:46:50 -0330 (NST)
Subject: Re: anhydrous polishing

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Glenn (and others)
I have polished B2O3 with the 3M lapping film and no water. It
doesn't produce a great polish, but you can usually find a few spots
where you can squeeze a beam in. If you don't use the 3M film, let me
know and I'll tell you where you can get some or even send you a scrap.
Maggy Piranian
M.U.N.

On Fri, 24 Feb 1995, Glenn Poirier wrote:

} Greetings Microscopists
}
} Is there anyone out there who has any tips/techniques on polishing hygroscopic
} material for microanalysis. Specifically, I need to polish some CaO
} particles with CaC rinds. I could probably polish them with silicone
} oil, but how do I get the oil off the samples before I put them in
} the microprobe? Any comments or suggestions would be appreciated.
}
} Thanks in advance
}
} Glenn
} **********************************************************************
} * Glenn Poirier glenn_p-at-geosci.lan.mcgill.ca *
} * Electron Microprobe Lab Phone: (514) 398 6774 *
} * Earth and Planetary Sciences Fax: (514) 398 4680 *
} * McGill University THERE ARE THREE SIDES TO EVERY STORY; *
} * Montreal, Quebec YOUR SIDE MY SIDE AND THE TRUTH *
} **********************************************************************
}




From: Miguel Avalos B. :      miguel-at-ifuname.ifisicaen.unam.mx
Date: Fri, 24 Feb 1995 19:51:33 -0800
Subject: Re: LKB Ultratome III

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From: smithj-at-acad.winthrop.edu
Date: Sat, 25 Feb 1995 20:55:59 -0500
Subject: Problems with 35mm T-max in TEM

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EM folk:
I'm trying to use T-max 100 in the 35mm camera on
my Zeiss EM 10C. Everything works well (you get nice
short exposures, and this particular camera can be
handled in the light, so there's no problem with using
a panchromatic film).
But the film gets *BRITTLE* in high vacuum. I have
snapped the film in half simply by bending it as I
unloaded the camera. Any hints, or do I just need to
switch to one of the orthochromatic films? If so, which
one(s) have you tried and liked?
Julian Smith III
Biology
Winthrop University
Rock Hill, SC
803-323-2111 (vox)





From: Dirk Knoesen, UWC, SA :      DIRK-at-physics.uwc.ac.za
Date: 27 Feb 95 08:45:52 GMT+0200
Subject: Re: TEM film

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Message-ID: {MAILQUEUE-101.950227084552.256-at-physics.uwc.ac.za}
To: microscopy-at-aaem.amc.anl.gov

Douglas Medlin wrote about fine cracks appearing on some of their TEM
negatives. I believe you are experiencing what photographers called
:crazing:. This is an effect that occurs when the temperature of the
developer and fixer is not the same, or at least the difference is
not small enough. It is exactly as you describe it, a fine line
structure on the film that looks that mud cracks.

Dirk Knoesen
Department of Physics, University Western Cape, Bellville, 7530
South Africa
e-mail: dirk-at-physics.uwc.ac.za
Phone: (+21) 959 2236 Fax: (+21) 959 3474




From: YANGA-at-NCCCOT.AGR.CA
Date: 27 Feb 1995 09:30:43 -0500 (EST)
Subject: RE:35mm film in TEM

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We use Eastman motion picture film (5302 fine grain release
positive film) in Philips EM300 in the past and currently in
Zeiss EM902 without any problem.

Ann Fook Yang




From: South Bay Technology :      73531.1344-at-compuserve.com
Date: 27 Feb 95 12:12:22 EST
Subject: anhydrous polishing/Abrasive Films

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One suggestion I would make would be polishing with abrasive lapping films.
These are abrasive particles which are bonded to a polyester film. It is a
higher grade of traditional SiC grinding paper and it comes in micronized
particle sizes. The material is designed to be used either with or without a
lubricant which should take care of your problem.

While it is always preferable to polish with some sort of lubricant, this is a
viable alternative for anhydrous materials. I must also add that the lapping
film is one of our products so I do have a vested interest in this suggestion.
If you would like a sample to try out, please contact me. It is avaialable in
Aluminum oxide down to .05 micron and in Diamond down to 0.1 micron. Typical
price for Al2O3 film is about $1.39 per 8" PSA Disc and for Diamond about $24
per 8" PSA disc. 8" Plain Back Diamond Film is more typically used and is
priced at about $20 per 8" Disc. Prices, of course, will decrease in quantity.
The diamond plain back films are used extensively in Tripo Polishing for SEM and
TEM applications.

Best regards-

David Henriks
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673

TEL: 800-728-2233
FAX: 714-492-1499






From: m.dickson-at-unsw.edu.au (Melvyn R. Dickson)
Date: Tue, 28 Feb 1995 12:50:49
Subject: Re: RE:35mm film in TEM

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To: microscopy-at-aaem.amc.anl.gov


I have used Kodak Fine Grain Release Positive (5302) in Philips microscopes
for 30 years. Never gave a problem with cracking with the unperforated (no
longer available) or perforated stock. Gave some problems with "lightning
strikes" on the final 4-5 frames out of 40. Philips have special cameras with
large diameter hubs so the film is never bent much and used unperforated film
so there was no tendency to crack at perforations. A 35 mm camera in our
Hitachi H-7000 gives very fine cracking across the film between the edges of
the perforations even when using the film Hitachi recommends. This can only
be avoided by using film which is only JUST dry enough, shooting the whole
roll and processing it in about 4 hours.







From: MatlsMicrs-at-aol.com
Date: Tue, 28 Feb 1995 02:24:41 -0500
Subject: Materials Microscopy

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For your information, Materials Microscopy is a new newsletter dedicated to
the professional interests of the working materials microscopist. Those who
wish to receive a free subscription should provide us with their full mailing
address via FAX -at- (602) 947-7615 or E-mail: MatlsMicrs-at-aol.com. Contributed
articles should be mailed to:
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Please contact me if you need any further information. Thank you.

Rene E. Nicholas
Circulation Manager
Materials Microscopy





From: desclinj-at-ulb.ac.be (Desclin Jean)
Date: Tue, 28 Feb 1995 08:25:56 +0100 (MET)
Subject: for Barbara Hartmann

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Hi Barbara!
I tried to no avail to send you the present message at your email
address: it stubbornly bounced back, so I eventually post it to the
microscopy list.
I apologize to the members of this list if they should feel this is
wasting bandwidth: just delete and go on to the next message ;-)

Barbara!
I am afraid I did not make my question clear enough ;-)
What I wanted to know was:
1) the testes you wish to fix are from what species ?

2) for what kind of microscopy are they to be processed ? I.e.
T.E.M., S.E.M., or light microscopy ?

3) What's the embedding medium?

4) in the case of light microscopy, do you contemplate using some special
technique (besides for recognizing the different stages of the
seminiferous epithelium) which would require that some specific
chemical would be either required or, on the contrary, avoided?

In my personal experience with rat testes and light microscopy (this
is rather very long ago: more than 30 years! :-( ), Bouin and other
picric acid containing variations thereof are not optimal fixatives
for preservation of the acrosomial apparatus of spermatids, which is
one of the features used by most classification methods (Clermont's
among others...).
The best fixatives for light microscopy of paraffin embedded tissue
are those containing potassium dichromate such as, for example,
Helly's fluid (Zenker + formaldehyde), but they also are followed, after
rinsing and dehydration, by shrinkage which is still more important than
that observed after Bouin's or Bouin-Hollande's (somewhat better) fluids.

BTW, what's wrong with picric acid? It is always shipped in 'moist
condition' in order to minimize hazards. I work in our lab since 1953,
with Bouin, Bouin-Allen, Bouin-Hollande, Dubosq-Brazil etc., every day,
and we never experienced any problem. We store the picric acid for our
needs in the form of a saturated aqueous solution which keeps for as long
as you will. This stock solution is then used for preparing all picric
acid containing fixatives.

There is no point in trying to eliminate the picric acid from the fixed
pieces of tissue before sectioning. If the presence of picric acid in the
slides should hamper subsequent staining (a rare occurrence, btw), then
the easiest and expeditious way to get rid of it is to pretend your tissue
was fixed with a sublimate containing fluid: at the rehydration step, your
slides should undergo treatment by alcoholic iodine (or lugol solution) and
sodium thiosulfate in the usual way and 'voila': no more picric acid on the
slides within 2 minutes!
Under no circumstances should the pieces of tissue fixed with a picric
acid containing fixative undergo rinsing in water! This is sheer heresy ;-)
because it induces a lot of swelling in the tissue before shrinking even
more during dehydration (I never found out which histologist ever advocated
such method, but I know quite a number of renowned ones who firmly condemned
it, and with good reason). If you insist on rinsing the pieces of testes after
fixation, then merely increasing the number of steps through 90x alcohol should
do the trick; you may even add a thin layer (1-2 mm thick) of lithium
carbonate on the bottom of the alcohol containing flasks: this helps
dissolving part of the picric acid away.

Possibly the method which should yield the best pictures has been documented
in a book entitled 'Histological and histopathological Evaluation of the
Testis', by Russell,Ettlin, Sinha Hikim and Clegg: Cache River Press 1990,
(15777 Bolesta, Box 129, Clearwater, Fl 34620): ISBN 09627422-0-1.

At the end of the book, there is an appendix about the materials and methods
which you might find useful (although perfusion fixation of rat testes is
among the most frustrating experiences I ever had, even with a lot of
heparin ). This would also require embedding in epoxy resin and making large
semi-thin sections, which I don't know whether you are prepared to do ;).
Glutaraldehyde certainly seems to me the best choice, but then paraffin
embedding would be ruled out (too hard and too brittle after paraffin
embedding).
HTH, and good luck.
Let me know about the outcome (good, I hope)
John
(correction: alcohol above should read 90 percent in volume)



***********************************************************
* Jean C. Desclin (John), Associate Prof. of Histology *
* Laboratory of Histology - Faculty of Medicine *
* Brussels Free University (U.L.B.) *
* e-mail: desclinj-at-ulb.ac.be (internet) *
* snail mail: route de Lennik 808 *
* B - 1070 Brussels Belgium *
***********************************************************




From: Ker{nen Jaakko-Tuomas :      jaakko-at-butler.cc.tut.fi
Date: Tue, 28 Feb 1995 12:14:33 +0200
Subject: Re: Materials Microscopy

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Yes, I want more information about the Materials Microscopy. Please
send me a free subscription.

Jaakko Keranen
Centre for electron microscopy
Tampere Univ. of technology
P.O.Box. 589
FIN-33101 Tampere
FINLAND




From: delannoy-at-welchlink.welch.jhu.edu (Michael Delannoy)
Date: Tue, 28 Feb 1995 09:38:53 -0500
Subject: lipid sizing and neg. stain

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To whomever,
Would someone please repost the recent info on lipid sizing (K.Walters?)
and the three responses on lipid negative staining. I had another crash and
lost
these responses.
Thanks
Mike D.





From: John F. Conroy :      John.Conroy-at-m.cc.utah.edu
Date: Fri, 24 Feb 1995 14:23:58 -0700 (MST)
Subject: Optical Microscopy of Tissues

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Message-Id: {MAILQUEUE-101.950228091038.320-at-ahabs.wisc.edu}




Hello,

I am currently writing a grant proposal, and I have been knocking my head
against the wall looking for some sort of data on the optical transmission
properties of nervous tissue (the brain). Does anyone have a good
reference, or, from a practical standpoint, how thick do brain tissue
slices have to be to obtain good optical transmission (esp. relative to
other fatty tissue slices like those from the earlobe, whose transmission
characteristics I can find since people have been doing pulse
oximetry.).

Thanks in advance,

John Conroy
University of Utah
Dept. Bioengineering


Dear John,

It has been years since I worked in this area, but I suggest you might
look at the literature for the optical transmission properties of retina.
As a nervous tissue, its optical properties should be quite similar to the
CNS as long as the measurements are not made in the vicinity of the fovea.
And, of course, due the importance of retinal transparency for vision, its
optical properties must be well known. For a place to start I'd have a
look at the "Handbook of sensory physiology" Springer-Verlag.

Just my two cents worth, Steven
-----------------------------------------------------------------------------
Steven L. Goodman, Ph.D.
Dept. Animal Health and Biomedical Sciences 608-262-0816 (office)
University of Wisconsin 608-262-7420 (FAX)
1655 Linden Drive
Madison, WI 53706 SLG-at-AHABS.WISC.EDU
--------------------------------------------------------------------------






From: delannoy-at-welchlink.welch.jhu.edu (Michael Delannoy)
Date: Tue, 28 Feb 1995 12:00:41 -0500
Subject: lipid sizing and neg. stain

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} Return-Path: {delannoy-at-welchlink.welch.jhu.edu}
} Received: from AAEM.AMC.ANL.GOV by welchlink.welch.jhu.edu (5.0/SMI-SVR4)
} id AA04845; Tue, 28 Feb 1995 09:52:41 -0500
} Date: Tue, 28 Feb 1995 09:38:53 -0500
} Message-Id: {9502281438.AA02618-at-welchlink.welch.jhu.edu}
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} Mime-Version: 1.0
} To: microscopy-at-aaem.amc.anl.gov
} From: delannoy-at-welchlink.welch.jhu.edu (Michael Delannoy)
} Subject: lipid sizing and neg. stain
} X-Mailer: {Windows Eudora Version 1.4.2b16}
} Content-Type: text/plain; charset="us-ascii"
}
} To whomever,
} Would someone please repost the recent info on lipid sizing
(K.Walters?)
} and the three responses on lipid negative staining. I had another crash and
} lost
} these responses.
} Thanks
} Mike D.
}
}





From: Miguel Avalos B. :      miguel-at-ifuname.ifisicaen.unam.mx
Date: Tue, 28 Feb 1995 10:37:19 -0800
Subject: Re: Materials Microscopy

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From: Damon Heer :      DLH-at-fei2.feico.com
Date: Tue, 28 Feb 1995 16:07:22 -0800
Subject: Used SEM for sale

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NANOMETRICS QWIKSCAN II
FESEM

$4,900

If interested, please contact Paxton Hong
InnfoGraphics
503 235-0227

Best regards,
Damon Heer

FEI Company
7451 N.E. Evergreen Parkway
Hillsboro, OR 97124-5830

Phone (503) 640-7582
fax (503) 640-7509
email dlh-at-feico.com




From: jfmjfm-at-umich.edu (John F. Mansfield)
Date: Tue, 28 Feb 1995 22:42:01 -0500
Subject: No link to Newsgroup

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Message-Id: {199503010340.WAA29098-at-srvr5.engin.umich.edu}
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Just a note to let you guys know that our link from this mailing list to
the Usenet newsgroup sci.techniques.microscopy has been broken. The
gateway at
sci.techniques.microscopy.usenet-at-decwrl.dec.com has been closed down. Dec
no longer support it.
If you want as much coverage for you questions and posts as possible I
encourage you to post both to this list and also to the Newssgroup.

If anyone knows how to set up a mail gateway to the Newsgroup, and also
from the Newsgroup to the mailing list, I would be very interested to hear
how to do it so we could reestablish the link and possibly make it
bi-directional this time!

Many thanks
Jfm.


______________
John Mansfield
North Campus Electron Microbeam Analysis Laboratory
413 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (313)936-3352 FAX (313)936-3352
jfmjfm-at-engin.umich.edu or John.F.Mansfield-at-umich.edu
URL: http://www.engin.umich.edu/~jfmjfm/jfmjfm.html






From: desclinj-at-ulb.ac.be (Desclin Jean)
Date: Wed, 1 Mar 1995 08:17:01 +0100 (MET)
Subject: to Barbara Hartman

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Hi Barbara!

I tried to no avail to send you the present message at your email
address: it stubbornly bounced back, so I eventually post it to the
microscopy list.
I apologize to the members of this list if they should feel this is
wasting bandwidth: just delete and go on to the next message ;-)

Barbara!
I am afraid I did not make my question clear enough ;-)
What I wanted to know was:
1) the testes you wish to fix are from what species ?

2) for what kind of microscopy are they to be processed ? I.e.
T.E.M., S.E.M., or light microscopy ?

3) What's the embedding medium?

4) in the case of light microscopy, do you contemplate using some special
technique (besides for recognizing the different stages of the
seminiferous epithelium) which would require that some specific
chemical would be either required or, on the contrary, avoided?

In my personal experience with rat testes and light microscopy (this
is rather very long ago: more than 30 years! :-( ), Bouin and other
picric acid containing variations thereof are not optimal fixatives
for preservation of the acrosomial apparatus of spermatids, which is
one of the features used by most classification methods (Clermont's
among others...).
The best fixatives for light microscopy of paraffin embedded tissue
are those containing potassium dichromate such as, for example,
Helly's fluid (Zenker + formaldehyde), but they also are followed, after
rinsing and dehydration, by shrinkage which is still more important than
that observed after Bouin's or Bouin-Hollande's (somewhat better) fluids.

BTW, what's wrong with picric acid? It is always shipped in 'moist
condition' in order to minimize hazards. I work in our lab since 1953,
with Bouin, Bouin-Allen, Bouin-Hollande, Dubosq-Brazil etc., every day,
and we never experienced any problem. We store the picric acid for our
needs in the form of a saturated aqueous solution which keeps for as long
as you will. This stock solution is then used for preparing all picric
acid containing fixatives.

There is no point in trying to eliminate the picric acid from the fixed
pieces of tissue before sectioning. If the presence of picric acid in the
slides should hamper subsequent staining (a rare occurrence, btw), then
the easiest and expeditious way to get rid of it is to pretend your tissue
was fixed with a sublimate containing fluid: at the rehydration step, your
slides should undergo treatment by alcoholic iodine (or lugol solution) and
sodium thiosulfate in the usual way and 'voila': no more picric acid on the
slides within 2 minutes!
Under no circumstances should the pieces of tissue fixed with a picric
acid containing fixative undergo rinsing in water! This is sheer heresy ;-)
because it induces a lot of swelling in the tissue before shrinking even
more during dehydration (I never found out which histologist ever advocated
such method, but I know quite a number of renowned ones who firmly condemned
it, and with good reason). If you insist on rinsing the pieces of testes after
fixation, then merely increasing the number of steps through 90x alcohol should
do the trick; you may even add a thin layer (1-2 mm thick) of lithium
carbonate on the bottom of the alcohol containing flasks: this helps
dissolving part of the picric acid away.

Possibly the method which should yield the best pictures has been documented
in a book entitled 'Histological and histopathological Evaluation of the
Testis', by Russell,Ettlin, Sinha Hikim and Clegg: Cache River Press 1990,
(15777 Bolesta, Box 129, Clearwater, Fl 34620): ISBN 09627422-0-1.

At the end of the book, there is an appendix about the materials and methods
which you might find useful (although perfusion fixation of rat testes is
among the most frustrating experiences I ever had, even with a lot of
heparin ). This would also require embedding in epoxy resin and making large
semi-thin sections, which I don't know whether you are prepared to do ;).
Glutaraldehyde certainly seems to me the best choice, but then paraffin
embedding would be ruled out (too hard and too brittle after paraffin
embedding).
HTH, and good luck.
Let me know about the outcome (good, I hope)
John
(correction for above glitch about the alcohol/ethanol: should read
alcohol 90 percent (in volume); I am still stuck with kermit and an
awfull editor :( ).



***********************************************************
* Jean C. Desclin (John), Associate Prof. of Histology *
* Laboratory of Histology - Faculty of Medicine *
* Brussels Free University (U.L.B.) *
* e-mail: desclinj-at-ulb.ac.be (internet) *
* snail mail: route de Lennik 808 *
* B - 1070 Brussels Belgium *
***********************************************************





From: desclinj-at-ulb.ac.be (Desclin Jean)
Date: Wed, 1 Mar 1995 08:46:40 +0100 (MET)
Subject: ignore, please

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this just is a test because it seems I am not able to reach the
list. Please ignore and delete. I apologize for the inconvenience.
desclinj-at-ulb.ac.be





From: jpawley-at-macc.wisc.edu (James Pawley)
Date: Wed, 1 Mar 1995 18:17:03 -0600
Subject: High resolution HVEM about to be trashed. Do you need it?

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The 1 MeV AEI EM-7 at the University of Wisconsin, Madison has been used
for the study of biological specimens for the past 23 years. In the early
1980's, almost every subsystem of this instrument (magnetic shielding and
ambient field, lens-current and HV stability, vibration isolation, LaB6
source, TV-interface, Axis-centered stereo-tilt stage, high-resolution
cold-stage etc.) was upgraded with a view to performing high-resolution
studies on frozen biological specimens. Images of oriented gold films at
that time showed 0.14 nm spots in bright field and using an 11 mm gap
(Pawley, J.B. (1984) Ultramicroscopy 13-4:387-406, describes most of these
improvements in detail.)

Because it seems probable that the present management of this instrument
will decide (has decided?) to decommission it in the very near future, (It
was almost scrapped over last Christmas), I am posting this notice in case
there are those that feel that the country has need of a second instrument
with the general specifications of the Berkeley ARM or even of the
very-stable Haefely 1-MEV supply that it contains.

The instrument is in operating condition, but, until recently, has received
less maintenance than it should have for the past five years or so.

The questions are:

1. Do you have important projects that require higher resolution (or a
large gap) than that available from 200-400kV instruments? (Keep in mind
that knock-on damage may be more severe at the higher voltage.)
2. Would you be willing to travel to Madison to obtain such facilities?
3. Would you be interested in relocating the instrument to a more
convenient location?

Bear in mind that, because the instrument requires a room 3 floors high and
a large (100 t) vibration-isolation block and also has substantial power
and cooling requirements, moving it would be a bit complex. However, it
could surely be done at less than 10% of $5M cost of the new Stuttgart 1.25
MeV instrument.

It would also need stage-rods more suited for material-science applications
but it is possible that the stage formerly fitted to the Cambridge HREM
could be fitted without great trouble as both instruments used the same
column.

The purpose of this communication is solely for the information of possible
users with the aim of avoiding anyone in the future saying "If only you had
told me?". I do not represent the Integrated Microscopy Resource where the
instrument is installed.

Please send any responses directly to me and do so ASAP:

jpawley-at-macc.wisc.edu (James Pawley)

***************NEW ADDRESS**************
Prof. James B Pawley, Ph. 608-263-3147
Room 1235, Engineering Research Building, FAX 608-265-5315
1500 Johnson Dr. Madison, Wisconsin, 53706.
JPAWLEY-at-MACC.WISC.EDU






From: Keith Moulding :      MCMOULDK-at-usthk.ust.hk
Date: 02 Mar 1995 15:38:58 +0800
Subject: Channel Software - EBSP

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Hi,

I have been trying to contact Niels-Henrik Schmidt (author of Channel). The
FAX number I have seems to be out of date.

Does anyone have Niels-Henrik Schmidt current FAX number and address?

Thanks in advance.


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

Dr. Keith Moulding,
Materials Characterisation and Preparation Centre,
Hong Kong University of Science and Technology,
Clear Water Bay,
Kowloon,
Hong Kong.

Tel: (852) 2358 8724
Fax: (852) 2358 2451

E-mail: mcmouldk-at-usthk.ust.hk

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~




From: andreas.brech-at-bio.uio.no (Andreas Brech)
Date: Thu, 02 Mar 1995 09:14:14 +0100
Subject: subscribe

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Andreas Brech
Electron Microscopical Unit for Biological Sciences
Department of Biology, University of Oslo.
P.O.Box 1062 Blindern
N-0316 Oslo 3
Norway
Tel.: + 43-22 85 61 89 (work)
+ 43-22 43 83 23 (privat)
Fax.: + 43-22 85 47 26
e-mail.: abrech-at-bio.uio.no





From: andreas.brech-at-bio.uio.no (Andreas Brech)
Date: Thu, 02 Mar 1995 09:18:32 +0100
Subject: subscribe

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Please, I would like to join the list.
Andreas Brech
Electron Microscopical Unit for Biological Sciences
Department of Biology, University of Oslo.
P.O.Box 1062 Blindern
N-0316 Oslo 3
Norway
Tel.: + 43-22 85 61 89 (work)
+ 43-22 43 83 23 (privat)
Fax.: + 43-22 85 47 26
e-mail.: abrech-at-bio.uio.no





From: NICK SCHRYVERS :      nick_schryvers-at-ruca.ua.ac.be
Date: 2 Mar 1995 14:59:45 +0100
Subject: HREM versus high ground wat

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Message-Id: {n1417965773.25625-at-ematserv.ruca.ua.ac.be}

REGARDING HREM versus high ground water

Does anyone has experience with high ground water levels troubeling HREM
operation?
Nick Schryvers
EMAT, RUCA
Antwerp, Belgium





From: tivol-at-tethys.ph.albany.edu
Date: Thu, 02 Mar 1995 10:35:15 EST
Subject: Electron backscattering cross-sections

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Does anyone have a referrence for backscattering cross-sections of electrons
in various substances as a function of energy and/or of angle? I'd really
appreciate a reply either to the list, by email or if I could get a table by
fax at (518) 474-8590. TIA.
Yours,
Bill Tivol
tivol-at-tethys.ph.albany.edu




From: Richard E. Edelmann :      EDELMARE-at-CASMAIL.MUOHIO.EDU
Date: Thu, 2 Mar 1995 10:58:56 -500
Subject: Freeze-Fracture

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I'm looking for any recomendations for freeze-fracture units.
We're putting together a grant here to obtain one and the only souce
I've come up with is Bal-tec. I am looking for other possible
vendors. Apparently JEOL has stopped production of their freeze-
fracture unit. Specifically we're looking for units comparable to
the Bal-Tec BAF 060 (not that I as of yet have anything against the
BAF 060, but it is nice to look around first.)

Thank you.

Richard E. Edelmann
Electron Microscopy Facility Supervisor
Miami University, Oxford, Ohio




From: T. Page Owen Jr :      tpowe-at-conncoll.edu
Date: Thu, 2 Mar 1995 13:25:27 -0500 (EST)
Subject: Re: Freeze-Fracture

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Cressington makes a nice freeze-fracture machine. I don't have the
address at hand, but I believe they are based in England.

Page Owen
Dept. of Botany
Connecticut College
New London, CT 06320






From: Dennis Shubitowski :      dennis%odin-at-odin.morph.med.umich.edu
Date: Thu, 2 Mar 1995 15:43:04 -0500 (EST)
Subject:

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Errors-To: {dennis-at-odin.morph.med.umich.edu}






From: M.V. Parthasarathy :      mvp2-at-cornell.edu
Date: Thu, 2 Mar 1995 13:43:38 -0400 (EDT)
Subject: RE: Freeze-Fracture

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Message-Id: {9503022058.AA09986-at-julian.uwo.ca}


SUBJECT: Freeze-fracture
Riichard,

I don't think there is a unit out as yet that can match the specs
and design of the new BAL-TEC unit. Cressington is another company which
manufactures freeze-fracture unit. While it may not have all the features of
the new BAL-TEC one, it is reported to be good for routine freeze-fracture.

*************************************************************************
M.V. Parthasarathy
Professor of Plant Biology
Section of Plant Biology
228 Plant Science Building
Cornell University, Ithaca, NY 14850
Telephone: (607) 255-1734
Fax: (607) 255-5407
E-mail: mvp2-at-cornell.edu
***********************************************************************




From: chandler-at-lamar.ColoState.EDU (John Chandler)
Date: Thu, 2 Mar 1995 11:58:20 -0700
Subject: Re: Freeze-Fracture

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Message-Id: {v01510100ab7bc4018e5c-at-[129.82.126.28]}
Mime-Version: 1.0
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} I'm looking for any recomendations for freeze-fracture units.
} We're putting together a grant here to obtain one and the only souce
} I've come up with is Bal-tec. I am looking for other possible
} vendors. Apparently JEOL has stopped production of their freeze-
} fracture unit. Specifically we're looking for units comparable to
} the Bal-Tec BAF 060 (not that I as of yet have anything against the
} BAF 060, but it is nice to look around first.)
}
} Thank you.
}
} Richard E. Edelmann
} Electron Microscopy Facility Supervisor
} Miami University, Oxford, Ohio

It looks as though you have some misinformation. JEOL is making the JFD
9000 series freeze-fracture instruments. I know that John Rash has
recently purchased and installed the 9010CR with the latest modifications,
including a specimen stage with rapid cooling.

Both Bal-Tec and JEOL make fine instruments and are worth looking at.


John chandler-at-lamar.ColoState.EDU Fort Collins CO






From: Jake Schaper :      Jake_Schaper-at-chdqm.sps.mot.com
Date: 2 Mar 1995 14:00:50 -0700
Subject: Subject-EM / AL/Si Stress

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Message-Id: {n1417969004.27274-at-chdqm.sps.mot.com}

Many published investigations on stress voids in aluminum/silicon alloys
report using CF4/O2 plasma as the deprocessing method to remove the final
PECVD oxynitride/ SiN. Does the use of this plasma chemistry generate its own
voids by removing silicon precipitates which in turn leave voids possibly
identified as stress voids? Precipitates that were in or near the correct
orientation give the appearance (typically wedge-shaped) of a stress void.
Optical inspection without deprocessing does not have adequate resolution.
I'd appreciate any comments on the CF4/O2 method and a possible alternative
method (other than argon backsputtering).

**********************************************************
Jake Schaper
Product Analysis Lab
Motorola
Chandler, Arizona
Phone 602-814-4756
**********************************************************






From: Dennis Shubitowski :      dennis%odin-at-odin.morph.med.umich.edu
Date: Thu, 2 Mar 1995 15:43:52 -0500 (EST)
Subject: Epoxy Resins: Accelerator?

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Errors-To: {dennis-at-odin.morph.med.umich.edu}

One of the divisions of our lab teases apart sural nerve tissue that have
been kept in a 1:1 mixture of epon:propylene oxide. The samples are very
easy to tease when the mixture is very fresh. The problem is that samples
became back-logged and were not able to be teased right away. A quick
decision had to be made, and the samples were frozen at -70C until they
could be teased.

The nerve fibers need to be infiltrated to be teased but not polymerized
or else they become too fragile. A problem is becoming evident that after a
long period of storage, the nerve samples are being fully infiltrated
and polymerized despite being frozen (which we thought would almost stop
the polymerization process because it is largely dependant on heat).
Realizing that hind-sight is 20/20, would it have been better to leave the
accelerator out of the epoxy mixture? What affect would this have on the
quality of the resin? I understand accelerator to act as a catalyst being
a part of the polymerization reaction but not really consumed. Any
opinions to offer?

Thanks,

Dennis








From: emlab-at-ucsco.ucsc.edu (Jon Krupp)
Date: Thu, 2 Mar 1995 11:12:14 -0800
Subject: Survey: Lab Changes

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Hi again:

I have been asked to make a brief (15 min) presentation at Scanning 95 in
Monterey, on March 30, 1995. The topic is 'Changing Role of the Microscopy
Lab in the University'. I know how my lab has changed, but would like to
get some idea from a broader sample of microscopists about changes in our
role etc.

My rough observations at this time include some of the following. More labs
seem to be consolidating, so we keep trying to catch up with too many
techniques, especially as staffs are reduced.

There are lots of other places for researchers to spend their funding than
before, we have lost lots of users in biology to molecular techniques. Some
of the new faculty see microscopy as 'too hard'.

There are fewer and fewer students who want to be 'microscopists'. Most
seem to want to come in on Monday, drop off something, and return on Friday
with a picture or two for their thesis.

The EM lab on our campus has evolved into an imaging and microscopy lab.
Because of our interest in images, we have always had the best darkrooms,
now we are trying to lead the way into digital imaging. This means more
computers and branching out from traditional 'microscopy'.

I could probably think of other changes, but that wouldn't leave anything
for you to add. Don't be shy, reply directly to me and I will try to
incorporate your comments in my presentation in a constructive way. Let me
know if you want specific credit for an idea, or if you prefer to have your
input blended for anonymity.

If you can't make it to Monterey, I'll send a summary if you ask.

Jonathan Krupp
Microscopy and Imaging Lab
University of California
Santa Cruz, CA 95060
emlab-at-ucsco.ucsc.edu
(408) 459-2477






From: John F. Conroy :      John.Conroy-at-m.cc.utah.edu
Date: Fri, 24 Feb 1995 14:23:58 -0700 (MST)
Subject: Optical Microscopy of Tissues

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Reply to: RE} Re: Optical Microscopy of Tissues
Dear John,
Sorry about the delay in responding - I missed your message in the deluge I
received while visiting labs for a few days. Check out the following papers on
infrared DIC-videomicroscopy.

H.-U. Dodt, W. Zieglgansberger (1990) Brain Research 537:333-356; (1994) Trends
in Neurosciences 17 (11): 453-458 (and references therein).

They looked with transmitted light at depths of up to 100 um in 300 um thick
sections. Used a 63x 1.4 NA oil immersion objective and DIC optics to visualize
nerve terminals. Note that the Nikon 60x 1.2 NA WATER immersion objective is
now available and has advantages in deep sections (see for example Mel
Brenner's application note in the November 1994 Journal of NIH Research).

Infrared penetrates well because water and tissues doesn't absorb it as much as
visible. For video work a CCD (or cooled CCD) is used because they are
sensitive to IR (unlike most tube cameras and your eye). DIC is the method of
choice because of optical sectioning. A general review or IR cameras is:
Silverman et al (March 1992) Scientific American 78-83 and 112-113. Any high
quality video CCD camera will work (as long as it does not have an IR blocking
filter!).

Sincerely,

Dr. George McNamara
Universal Imaging Corporation
--------------------------------------

Hello,

I am currently writing a grant proposal, and I have been knocking my head
against the wall looking for some sort of data on the optical transmission
properties of nervous tissue (the brain). Does anyone have a good
reference, or, from a practical standpoint, how thick do brain tissue
slices have to be to obtain good optical transmission (esp. relative to
other fatty tissue slices like those from the earlobe, whose transmission
characteristics I can find since people have been doing pulse
oximetry.).

Thanks in advance,

John Conroy
University of Utah
Dept. Bioengineering

---------
Dear John,

It has been years since I worked in this area, but I suggest you might
look at the literature for the optical transmission properties of retina.
As a nervous tissue, its optical properties should be quite similar to the
CNS as long as the measurements are not made in the vicinity of the fovea.
And, of course, due the importance of retinal transparency for vision, its
optical properties must be well known. For a place to start I'd have a
look at the "Handbook of sensory physiology" Springer-Verlag.

Just my two cents worth, Steven






From: NICK SCHRYVERS :      nick_schryvers-at-ruca.ua.ac.be
Date: 3 Mar 1995 08:26:09 +0100
Subject: obj. lens 100C replacement

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Message-Id: {n1417902813.11975-at-ematserv.ruca.ua.ac.be}

REGARDING obj. lens 100C replacement

The objective lens and the upper and lower column parts of our 20 year old
side entry JEOL 100 C microscope need replacement. New parts, however, are
rather expensive in view of the age of the instrument so we're looking for
anyone who has some second hand spare parts for sale. Sincerely,
Nick Schryvers tel: 32-3-2180247 fax: 32-3-2180257
EMAT, University of Antwerp
Groenenborgerlaan 171
B-2020 ANTWERP
Belgium






From: richard.easingwood-at-stonebow.otago.ac.nz (Richard Easingwood)
Date: Fri, 3 Mar 1995 17:37:52 +1100
Subject: Resins: BDMA vs DMP-30

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A message from Allan Mitchell:

The BDMA versus DMP-30 debate continues.

I first became aware of the recomendation to replace the epoxy resin=
acelerator DMP-30 with the acelerator BDMA when Audrey Glauert published=
her article "Accelerators for epoxy resins" in the RMS Proceedings (Sept, 1=
987).

The reason offered to encourage the switch from DMP-30 to BDMA was the fact=
that BDMA is less viscous, therefore diffuses into the tissue sample=
better. This gives a more even polymerisation and better sectioning. BDMA=
also has a longer shelf life.

I followed Glauert's recomendation, replacing DMP-30 with BDMA in our resin=
formula's. However, Audrey Glauert did not mention in the article that=
we should double the quantity of BDMA in the formulation when switching=
from DMP-30 to BDMA.

I replaced the DMP-30 with BDMA, using the BDMA at the same concentration as=
I had used the DMP-30.

Generally, it worked fine. Occasionally I had 'strange embedding' problems,=
ie samples not properly polymerised.

It then came to my attention that I should be using BDMA at twice the DMP-30=
concentration. I did not pay much attention to this at the time as=
generally everything had been working fine and I was to busy to experiment=
with the change.

After recent 'flow' on the List server the topic again surfaced i.e. I=
should be using BDMA at twice the DMP-30 concentration. This I have now tr=
ied.

My problem is that if I do an overnight infiltration in resin with the=
increased concentration of BDMA the resin is so viscous next morning it=
won't even come out of the vial when tipped upside down. To change to=
fresh resin is almost impossible, in fact it is easier to change the=
samples to fresh resin in a new processing tube. Unfortunately even then=
it is not totally successful as a large 'blob' of tacky resin stays with=
the sample.

I did a little experiment as outlined below;

1. Made up resin with normal DMP-30 concentration.
2. Made up resin with BDMA at normal DMP-30 concentration.
3. Made up resin with BDMA at twice DMP-30 concentration.

Results

Viscosity (by eye) Colour
To start with
DMP-30 most viscous slightly orange
BDMA (-at- DMP-30 conc) least viscous straw coloured
BDMA (-at- 2 x DMP-30 conc)similar to BDMA -at- DMP 30 conc straw coloured

After 5 hours
DMP-30 least viscous darker straw coloured
BDMA (-at- DMP-30 conc) similar to DMP-30 straw coloured
BDMA (-at- 2 x DMP-30 conc)quite viscous straw coloured

Overnight
DMP-30 least viscous straw coloured
BSMA (-at- DMP-30 conc) slightly more viscous than DMP-30 straw coloured
BDMA (-at- 2 x DMP-30 conc)very very viscous straw coloured

After the overnight step both the DMP-30 mix and the BDMA mix at the DMP-30=
concentration were quite acceptable, ie no difficulty replacing the old=
resin with fresh resin.

The BDMA mix at the twice DMP-30 concentration ws very very tacky.

After polymerisation both the DMP-30 and the BDMA -at- the DMP-30=
concentration are a light straw colour (normal expectation). The BDMA -at-=
twice the DMP-30 concentration was slightly darker, although still an=
acceptable straw colour.

By modifying my processing schedule such that the samples stay in 3 parts=
plastic and 1 part propylene oxide overnight I can successfully process a=
sample however, I am a little unhappy with the short time the sample is in=
'resin only' the following day, ie out of 3;1 and into fresh plastic first=
thing in the morning, embedded and in the oven before I go home. With a=
lot of samples to embed at times trying to give the samples as long as=
possible in the 'resin only' makes the end of the day a bit tight for time=
.

The problem becoming particularly accute when using our Lynx Automatic=
Tissue processor. If using BDMA -at- twice the DMP-30 concentration I cannot=
leave the resin in the processer overnight as it is to tacky the next day.

My Question ??? (at last some may say)

1. How are others out there, who are using BDMA at twice the DMP-30=
concentration getting around the problem of the resin going very tacky=
overnight, during infiltration?

What sort of infiltration times do you use?


2. Are any of you using BDMA at twice the DMP-30 concentration in an=
automatic tissue processor?

3. Is any one else having problems with the resin if made up with BDMA at=
twice the DMP-30 concentration?



Thank you in anticipation.


Allan Mitchell
South Campus Electron Microscope Unit
Department of Anatomy and Structural Biology
Otago Medical School










From: A. Kent Christensen :      akc-at-umich.edu
Date: Fri, 3 Mar 1995 08:21:39 -0500 (EST)
Subject: Re: Epoxy Resins: Accelerator?

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Dennis Shubitowski {dennis-at-odin.morph.med.umich.edu}

Dennis,

If you omit the accelerator, it would probably work well. We use
Epon-Araldite (Polyscience kit), and we routinely store the mixed resin
(Epon + Araldite + DDSA), without catalyst (DMP-30), in a 4 degree C
refrigerator. It will remain good for at least 6 months. When we are
ready to embed, we measure out whatever volume we want, add DMP-30 to
make 2%, mix, and it is fine. If you stored your nerve samples in 1:1
Epon (no accelerator):PO, and later decided to embed them, you could then
embed them by standard procedures with catalyzed resin. If you have any
questions about this, give me a call (763-1287), or drop over (2 blocks
away).

Kent

A. Kent Christensen
Department of Anatomy and Cell Biology
University of Michigan Medical School
Ann Arbor, MI 48109-0616
{akc-at-umich.edu}

----------------------------------------

On Thu, 2 Mar 1995, Dennis Shubitowski wrote:

} One of the divisions of our lab teases apart sural nerve tissue that have
} been kept in a 1:1 mixture of epon:propylene oxide. The samples are very
} easy to tease when the mixture is very fresh. The problem is that samples
} became back-logged and were not able to be teased right away. A quick
} decision had to be made, and the samples were frozen at -70C until they
} could be teased.
}
} The nerve fibers need to be infiltrated to be teased but not polymerized
} or else they become too fragile. A problem is becoming evident that after a
} long period of storage, the nerve samples are being fully infiltrated
} and polymerized despite being frozen (which we thought would almost stop
} the polymerization process because it is largely dependant on heat).
} Realizing that hind-sight is 20/20, would it have been better to leave the
} accelerator out of the epoxy mixture? What affect would this have on the
} quality of the resin? I understand accelerator to act as a catalyst being
} a part of the polymerization reaction but not really consumed. Any
} opinions to offer?
}
} Thanks,
}
} Dennis
}
}
}
}
}




From: gwerdos-at-gnv.ifas.ufl.edu (Greg Erdos ICBR EM Core Lab)
Date: Fri, 03 Mar 1995 09:09:34 -0500 (EST)
Subject: Re: Epoxy Resins: Accelerator?

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MICROSCOPY-at-AAEM.AMC.ANL.GOV
Cc: Dennis Shubitowski {dennis-at-odin.morph.med.umich.edu}
Message-id: {01HNOZPUD02Q93M845-at-gnv.ifas.ufl.edu}
MIME-version: 1.0
X-Mailer: Windows Eudora Version 1.4.4
Content-type: text/plain; charset="us-ascii"
Content-transfer-encoding: 7BIT

In reference to Dennis Subitowksi's question about expoxt
accelerator, I can say that we routinely exclude the accelerator during
infiltration with Epon, Epon/Araldite mixture and Epon substitutes and add
it only to the second pure resin infiltration step. This allows long
infiltrations in earleir steps with difficult specimens. When we have
compared adding it to all steps, the results seem to be the same.
Hope that helps

*****ORIGNINAL MESSAGE BELOW********
} One of the divisions of our lab teases apart sural nerve tissue that have
} been kept in a 1:1 mixture of epon:propylene oxide. The samples are very
} easy to tease when the mixture is very fresh. The problem is that samples
} became back-logged and were not able to be teased right away. A quick
} decision had to be made, and the samples were frozen at -70C until they
} could be teased.
}
} The nerve fibers need to be infiltrated to be teased but not polymerized
} or else they become too fragile. A problem is becoming evident that after a
} long period of storage, the nerve samples are being fully infiltrated
} and polymerized despite being frozen (which we thought would almost stop
} the polymerization process because it is largely dependant on heat).
} Realizing that hind-sight is 20/20, would it have been better to leave the
} accelerator out of the epoxy mixture? What affect would this have on the
} quality of the resin? I understand accelerator to act as a catalyst being
} a part of the polymerization reaction but not really consumed. Any
} opinions to offer?
}
} Thanks,
}
} Dennis
}
}
}
}
}
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Greg Erdos Phone: 904-392-1295
Scientific Director, ICBR EMCL
218 Carr Hall Fax 904-846-0251
University of Florida E-mail: gwerdos-at-gnv,ifas.ufl.edu
Gainesville, FL 32611





From: rutledge phil :      prutle1-at-gl.umbc.edu
Date: Fri, 3 Mar 1995 12:28:33 -0500
Subject: Electron Microscopes for Sale

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Interested parties:

Due to renovations within the department, and the purchase of a confocal
microscope, we have 2 transmission EMs in excess of our needs that we
would like to move. Both microscopes are in good working condition, one
has been under a service contract until this past year (though lightly
used). The scopes:

1. Hitachi H-600 STEM (the one under service contract until recently)

2. Zeiss EM-9S TEM

We are not quite at a "No reasonable offer refused!" price level, but we
would like to see these scopes get some more use and we're eager to make
use of the space they are now occupying. So....if you are at all
interested, contact me and we can discuss the particulars and terms
of sale.

TIA,

Phil Rutledge
voice: (410) 455-3582
Email: prutle1-at-gl.umbc.edu
Fax: (410) 455-3875




From: Jay Jerome :      jjerome-at-isnet.is.wfu.edu
Date: Fri, 3 Mar 1995 10:40:16 -0500 (EST)
Subject: Re: Epoxy Resins: Accelerator?

Contents Retrieved from Microscopy Listserver Archives
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Dennis Shubitowski {dennis-at-odin.morph.med.umich.edu}

We had a similar experience some years ago. Not with nerve, but with
other material. I can offer the following humble comments.

1. As you learned, resin does polymerize in even at freezing
temperatures. We have not tried -70 C and you did not specify how far
below freezing you stored your specimens. We did not test exactly why,
but reasoned that since the reaction is highly exothermic and since both
resin and tissue are good insulators, it is probable that local areas are
heated enough to allow polymerization. I look forward to any comments on
the errors in our reasoning, since this was only a guess to explain the
results.
2. Leaving out the accelerator does tremendously slow down the reaction,
but polymerization still occurs in absence of accelerator, and even in
the cold, so don't store for grossly extended times. To polymerize
infiltrate tissue with fresh resin containing accelerator (we use 2
changes, 4 hours each) after thawing tissue. This works well, although
some areas may have obvious interfaces between resins of different
hardness.


Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-isnet.is.wfu.edu *
**************************************************************

On Thu, 2 Mar 1995, Dennis Shubitowski wrote:

} One of the divisions of our lab teases apart sural nerve tissue that have
} been kept in a 1:1 mixture of epon:propylene oxide. The samples are very
} easy to tease when the mixture is very fresh. The problem is that samples
} became back-logged and were not able to be teased right away. A quick
} decision had to be made, and the samples were frozen at -70C until they
} could be teased.
}
} The nerve fibers need to be infiltrated to be teased but not polymerized
} or else they become too fragile. A problem is becoming evident that after a
} long period of storage, the nerve samples are being fully infiltrated
} and polymerized despite being frozen (which we thought would almost stop
} the polymerization process because it is largely dependant on heat).
} Realizing that hind-sight is 20/20, would it have been better to leave the
} accelerator out of the epoxy mixture? What affect would this have on the
} quality of the resin? I understand accelerator to act as a catalyst being
} a part of the polymerization reaction but not really consumed. Any
} opinions to offer?
}
} Thanks,
}
} Dennis
}
}
}
}
}





From: EMLAB-at-opus.mco.edu
Date: Fri, 03 Mar 1995 16:09:13 -0400 (EDT)
Subject: Tissue processors

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From: Michael Rock :      merock-at-u.washington.edu
Date: Fri, 3 Mar 1995 14:23:47 -0800 (PST)
Subject: Re: Resins: BDMA vs DMP-30

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Richard-
Why not try infiltrating your samples overnight in 100% resin with the lower
concentration of BDMA, then swithching to a "short" infiltration with
fresh resin made with 2X concentration BDMA. then embedding in the higher
concentration of BDMA in fresh resin?
just a hunch.
-Mike




From: JOHNA-at-SCI.WFEB.EDU
Date: Fri, 03 Mar 1995 17:14:50 -0400 (EDT)
Subject: BDMA vs DMP-30

Contents Retrieved from Microscopy Listserver Archives
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I performed a similar set of experiments: BDMA vs DMP-30. Although it
infiltrated specimens adequately and although they sectioned fine;
dealing with changes of 100% plastic that were sooooo viscous was more than
I could tolerate. In spite of it's initial higher viscosity and shorter
shelf life, I switched back to using DMP-30 in our PolyBed 812 resin.
Doing changes of 100% plastic is no longer a headache and something to be
dreaded. If someone has a solution to the overnight BDMA-plastic blob
problem, I'd like to hear as well.

Cheers...........JohnA

___________________________________________________
| |
| John G. Aghajanian, Ph.D. |
| Worcester Foundation for Experimental Biology |
| 222 Maple Avenue |
| Shrewsbury, MA 01545-2737 |
| |
| Tel: 508 842-8921 ext. 147, 161 |
| Fax: 598 842-9632 |
| JOHNA-at-sci.wfeb.edu |
| |
|_________________________________________________|





From: Greg Erdos ICBR EM Core Lab Univers :      GWERDOS-at-gnv.ifas.ufl.edu
Date: Fri, 03 Mar 1995 22:48:06 -0500 (EST)
Subject: Fw: Electron Microscopes

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Dear EM Brothers & Sisters,

This message came to me and I have no good answers. Any comments will
be appreciated and I will forwrad to the questioners
=
=Greg,
=
=Tom Mareci suggested that you might be able to give us some technical
=advice on EM's to help us solve a temporary problem we're having.
=
=We're wanting to move our 4.7T magnet to a location in the ARB basement
=120' from Dr. Craig Tisher's EM facility, which has a Zeiss EM 10 SEM
=and a Topcon/International Scientific Instruments DS-130C TEM. The SEM
=specs {= 3 mG AC and {= 5 mG DC. The TEM specs {=6 mG AC. Dr. Tisher
=is reluctant to have us install our magnet, which with shileding is
=calculated to produce a DC magnetic field of about 17 mG in the EM room.
=Tom and I are having a hard time understanding the DC spec, in light of
=the earth's magnetic field being 100x greater than the 5 mG spec.
=We are also finding it difficult to get good scientifically sound
=answers from the EM manufacturers (at least that we NMR people can
=understand!). Could you enlighten us?
=
=Our main questions are: Will our DC field of 17 mG really pose a problem
=for the EM's? If so, how can it be remedied? How much will it cost?
=How are these systems currently shielded/compensated for the earth's
=magnetic field? (My guess is that at most, a quick realignment of a
=passive shield will be all that's required, if there's an observable
=effect.)
=
=Secondarily, since before and after image quality may be the best bottom-
=line test of the effect (if any) of our magnet, what standard samples can
=you recommend for these Q.C. checks?
=
=Thanks for your advice and comments.
=
=-Richard Briggs
=
=tel: 395-0680 ext 54279 (55-4279)
=FAX: 395-0279
=pager: Shands # 3497
=e-mail: rbriggs-at-ufnmr.health.ufl.edu
=
=P.S.: You may not remember, but I met you at Tom's Christmas party.
=
******************************************************
* Greg Erdos ** *
* Director, ICBR EMCL ** Phone 904-392-1295 *
* 218 Carr Hall ** FAX 904-846-0251 *
* University of Florida ** gwerdos-at-gnv.ifas.ufl.edu *
* Gainesville, FL 32611 ** *
******************************************************************




From: images1-at-biosci.mbp.missouri.edu (Michael Stanley)
Date: Sat, 4 Mar 1995 12:15:37 -0600
Subject: sheep ovary

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Hello All,
I have a user that is attempting double-label fluroescence for
protein localization in sheep ovaries. The ovaries seem to auto-fluoresce
very strongly in the FITC channel with some signal in the Rhodamine as
well. They have talked to other researchers who have not seen this
problem. The tissue is fixed in Carnoy's and paraffin embedded.
Has anyone seen or heard of this particular tissue lighting up like
a Christmas Tree?
TIA,

C. Michael Stanley, Ph.D.
Coordinator, Associate Director
Molecular Cytology Core Facility
Molecular Biology Program
2 Tucker Hall
University of Missouri-Columbia
Columbia, MO. 65211
(314) 882-4895
fax= 314-882-0123






From: xin yang li :      xl48-at-uow.edu.au
Date: Sun, 5 Mar 1995 22:14:58 +1000 (EST)
Subject: subscribe

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Hi, everyone there.

I have changed my e-mail address because the up date of the system here. I'd
like to keep subscribing information from you.

My old address:g9177248-at-wumpus.cc.uow.edu.au

My new address:xl48-at-wumpus.cc.uow.edu.au

Thanks

Xinyang Li




From: MatlsMicrs-at-aol.com
Date: Sun, 5 Mar 1995 14:39:46 -0500
Subject: Materials Microscopy

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In response to my invitation for a free subscription to Materials Microscopy
(posted on Feb. 24), we have been receiving a large number of requests and
inquiries on a daily basis via e-mail and FAX. We will try to process all
your subscription requests prior to the circulation of our next issue. Due
to the high volume of mail we have received, I cannot respond to your
inquiries individually. However, I have tried to summarize, itemize, and
respond to your most frequently asked questions as follows:
a. Materials Microscopy is published and circulated at no cost among the
materials microscopy community members by Promotech Associates, Inc.
b. Our publication is mainly concerned with providing articles and material
of interest to working materials microscopist. We would appreciate your
contribution which meets this requirement. Further information in this
regard can be obtained by contacting our technical editor, Dr. Patricia Labun
(a materials microscopist).
c. I will send a copy of our "rate sheet" to those of you who expressed
interest in placing ads or promotional material. Please feel free to contact
me if you require any additional info.
Thank you all for your interest in Materials Microscopy.

Rene E. Nicholas
Circulation/Sales Manager

Materials Microscopy
P.O. Box 2014
Scottsdale, AZ 85252

TEL (602) 947-7603
FAX (602) 947-7615
MatlsMicrs-at-aol.com




From: A. Kent Christensen :      akc-at-umich.edu
Date: Sun, 5 Mar 1995 15:52:57 -0500 (EST)
Subject: Re: Resins: BDMA vs DMP-30

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MICROSCOPY-at-AAEM.AMC.ANL.GOV

I'm wondering if the possible advantages of BDMA (lower viscosity,
longer shelf life) are worth all this. I'm still using a 1989 Polysciences
Epon-Araldite kit (with DMP-30) that still gives excellent results with
standard procedures.

A. Kent Christensen
Department of Anatomy and Cell Biology
University of Michigan Medical School
{akc-at-umich.edu}




From: desclinj-at-ulb.ac.be (Desclin Jean)
Date: Mon, 6 Mar 1995 08:25:18 +0100 (MET)
Subject: fixation of testes

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Hi Barbara!
I tried to no avail to send you the present message at your email
address: it stubbornly bounced back, so I eventually post it to the
microscopy list.
I apologize to the members of this list if they should feel this is
wasting bandwidth: just delete and go on to the next message ;-)

Barbara!
I am afraid I did not make my question clear enough ;-)
What I wanted to know was:
1) the testes you wish to fix are from what species ?

2) for what kind of microscopy are they to be processed ? I.e.
T.E.M., S.E.M., or light microscopy ?

3) What's the embedding medium?

4) in the case of light microscopy, do you contemplate using some special
technique (besides for recognizing the different stages of the
seminiferous epithelium) which would require that some specific
chemical would be either required or, on the contrary, avoided?

In my personal experience with rat testes and light microscopy (this
is rather very long ago: more than 30 years! :-( ), Bouin and other
picric acid containing variations thereof are not optimal fixatives
for preservation of the acrosomial apparatus of spermatids, which is
one of the features used by most classification methods (Clermont's
among others...).
The best fixatives for light microscopy of paraffin embedded tissue
are those containing potassium dichromate such as, for example,
Helly's fluid (Zenker + formaldehyde), but they also are followed, after
rinsing and dehydration, by shrinkage which is still more important than
that observed after Bouin's or Bouin-Hollande's (somewhat better) fluids.

BTW, what's wrong with picric acid? It is always shipped in 'moist
condition' in order to minimize hazards. I work in our lab since 1953,
with Bouin, Bouin-Allen, Bouin-Hollande, Dubosq-Brazil etc., every day,
and we never experienced any problem. We store the picric acid for our
needs in the form of a saturated aqueous solution which keeps for as long
as you will. This stock solution is then used for preparing all picric
acid containing fixatives.

There is no point in trying to eliminate the picric acid from the fixed
pieces of tissue before sectioning. If the presence of picric acid in the
slides should hamper subsequent staining (a rare occurrence, btw), then
the easiest and expeditious way to get rid of it is to pretend your tissue
was fixed with a sublimate containing fluid: at the rehydration step, your
slides should undergo treatment by alcoholic iodine (or lugol solution) and
sodium thiosulfate in the usual way and 'voila': no more picric acid on the
slides within 2 minutes!
Under no circumstances should the pieces of tissue fixed with a picric
acid containing fixative undergo rinsing in water! This is sheer heresy ;-)
because it induces a lot of swelling in the tissue before shrinking even
more during dehydration (I never found out which histologist ever advocated
such method, but I know quite a number of renowned ones who firmly condemned
it, and with good reason). If you insist on rinsing the pieces of testes after
fixation, then merely increasing the number of steps through 90pc x alcohol should
do the trick; you may even add a thin layer (1-2 mm thick) of lithium
carbonate on the bottom of the alcohol containing flasks: this helps
dissolving part of the picric acid away.

Possibly the method which should yield the best pictures has been documented
in a book entitled 'Histological and histopathological Evaluation of the
Testis', by Russell,Ettlin, Sinha Hikim and Clegg: Cache River Press 1990,
(15777 Bolesta, Box 129, Clearwater, Fl 34620): ISBN 09627422-0-1.

At the end of the book, there is an appendix about the materials and methods
which you might find useful (although perfusion fixation of rat testes is
among the most frustrating experiences I ever had, even with a lot of
heparin ). This would also require embedding in epoxy resin and making large
semi-thin sections, which I don't know whether you are prepared to do ;).
Glutaraldehyde certainly seems to me the best choice, but then paraffin
embedding would be ruled out (too hard and too brittle after paraffin
embedding).
HTH, and good luck.
Let me know about the outcome (good, I hope)
John



***********************************************************
* Jean C. Desclin (John), Associate Prof. of Histology *
* Laboratory of Histology - Faculty of Medicine *
* Brussels Free University (U.L.B.) *
* e-mail: desclinj-at-ulb.ac.be (internet) *
* snail mail: route de Lennik 808 *
* B - 1070 Brussels Belgium *
***********************************************************





From: Marcel.Paques-at-2488PAS.urlnl.sprint.com
Date: Mon, 6 Mar 1995 04:42:00 -0500
Subject: freeze fracture

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X400-Received: by mta merit in /PRMD=internet/ADMD=telemail/C=us/; Relayed; Mon, 6 Mar 1995 04:53:08 -0500
X400-Received: by /ADMD=TELEMAIL/C=US/; Relayed; Mon, 6 Mar 1995 04:38:08 -0500
X400-Received: by /PRMD=SMXFL2/ADMD=TELEMAIL/C=US/; Relayed; Mon, 6 Mar 1995 04:42:47 -0500
X400-Received: by /PRMD=LANGATE/ADMD=TELEMAIL/C=GB/; Relayed; Mon, 6 Mar 1995 04:42:00 -0500

Cressington makes an excellent freeze fracture machine. Suitable for
routine work and high resolution shadowing at very low temperatures.
In particular the reproducibility, even of W/Ta, is very good, and
attainable for everyone (user friendly).

The address is:
Dr Peter A Walley
CRESSINGTON Scientific Instruments Ltd
24 Chalk Hill Watford Herts WD1 4BX UK
Tel 0923 220499
Fax 0923 816646

Success

Marcel Paques
Unilever Research Laboratory Vlaardingen
The Netherlands
E-mail: Marcel.Paques-at-2488PAS.URLNL.Sprint.Com





From: Kevin H Jennings :      Kevin_H_Jennings%notes-at-sb.com
Date: 6 Mar 95 13:28:00 ES
Subject: freeze-fracture

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Message-Id: {9503061636.AA1118-at-pho018.sb.com}
To: Microscopy {Microscopy-at-aaem.amc.anl.gov}

Further to the comments made concerning Cressington - they also have a US
distributor:

Alan Berginc
Cressington Scientific Instruments
508 Thomson Drive
Cranberry TWP
Pittsburgh
PA16066-6425

Tel: 412-772-0220
Fax: 412-772-0219

We use a Cressington CFE- 50B which has proved excellent for reproducible
rotary low-angle shadowing of peptides. The system is also very flexible for
both W/Ta and Pt/C operation and is easily re-configured for a variety of
research applications.


Kevin Jennings
SmithKline Beecham Pharmaceuticals UK
Microscopy & Flow Cytometry Section
The Frythe
Welwyn
Hertfordshire
AL6 9AR
United kingdom

e-mail Kevin_H_Jennings%Notes-at-SB.com





From: EMLAB-at-opus.mco.edu
Date: Mon, 06 Mar 1995 09:22:27 -0400 (EDT)
Subject: TISSUE PROCESSORS

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Dear biological TEM'ers,

I am in the info gathering stage of purschasing a tissue processor for
EM samples. I have info on the Reichert-Lynx unit and the one made by RMC.
How do you like these units? Comments on advantages and disadvantages are
welcome.
I also have brouchers on a unit made by Sakura Finetek USA, Inc..
The phone number I called is no longer in working order.(213-539-5441)
Is this company still around? These brouchers are probably from the early 80's.
Are the any other companys that made tissue processors?

Thanks in advance

Ed Calomeni
Medical College of Ohio
Toledo, OH

emlab-at-opus.mco.edu

PS Sorry about the post on last friday with no message in the body. Computers
can be such good friends.




From: Christine Powers :      cp-at-insitu.ummed.edu
Date: Mon, 6 Mar 1995 10:08:00 -0500 (EST)
Subject: TEM-Cell culture monolayers

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I recently read W. L. Steffens comments regarding the use of Leighton
tubes for embedding cell cultures. Has anyone embedded the coverslips from
these tubes in LRWhite resin? I've been having trouble getting consistent
polymerization of monolayers on other coverslips (eg Thermanox) and am in
search of a new method for obtaining "en face" sections of monolayers for
immunocytochemistry. TIA for any hints on the topic.

Christine Powers
Dept. of Cell Biology
University of Massachusetts Medical Center
Worcester, MA 01655




From: W.L. Steffens :      STEFFENS.B-at-calc.vet.uga.edu
Date: Mon, 6 Mar 1995 10:06:02 EST
Subject: Tissue Processors

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Ed,
I can comment on the Lynx tissue processor which we have been using
since it first came out, about 8 years ago. The Lynx is made in
Australia, and was originally distributed in this country by Fairleigh-
Dickinson Laboratories until the distributorship was snatched up by
Reichert (now Leica). We paid about 5.5K for the unit and have had little
trouble with it aside from a blown power supply (they have upgraded this)
and a defective exhaust fan (we fixed this). We use it routinely for
processing TEM (epon and L.R. White) and SEM (through dehydration). It
works very well is quite consistent.
My only complaint involves the expendables for it...vials, caps,
baskets, etc. Originally, it was economical to use them once and dispose
of them as is intended. Once Leica became the distributor, the price of
the expendable essentially tripled...some things quadrupled. As a result,
we clean and recycle these components for as long as we can.
Additionally, the quality control of these items has suffered immensely.
There are now occasional vials that are distorted and won't fit the
turntable, and lids and baskets with burrs that must be filed.
If you can accept this, I still think that for the money (nearly 9K by
now?) its the best one out there. Good Luck.

-=W.L. Steffens=-
College of Veterinary Medicine
University of Georgia




From: Beth Trend :      trend-at-cems.umn.edu
Date: Mon, 6 Mar 1995 11:07:06 -0600
Subject: TEM: JEOL100CX for sale

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We have a JEOL 100CX scanning transmission electron microscope that we no=
longer
need because of more recent acquisitions. It was maintained continuously=
during
operation here and was in perfect working condition before we turned it o=
ff in =

July, 1994. =


This is a conventional scanning transmission electron microscope with =

resolutions of 3.4 =81 TEM.

It has specimen holders for heating-cooling, double tilt (tilt range is =B1=
45=A1), =

or rotation. =

We would be deligted to entertain offers for it.

Please contact Beth Trend if you are interested.




______________________________________________***************************=
*******
Beth Trend trend-at-cems.umn.edu or btrend-at-maroon.tc.=
umn.edu
Coordinator, Characterization Facility University of Minnesota
Center for Interfacial Engineering =

100 Union St SE Room 187 phone 612-624-1365 fax 612-626-7530=

Minneapolis, MN 55455 =






From: Gib Ahlstrand :      giba-at-puccini.crl.umn.edu
Date: Mon, 6 Mar 1995 12:13:29 -0600
Subject: BDMA vs DMP-30 summary

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In July and September of 1994, there was some disciussion of BDMA vs. DMP-30. I
saved and consolidated 13 of those messages into a single document. If anyone is
interested in getting a copy, send me your e-mail address and I will zip one out
to you within a day or two.

--

Gib Ahlstrand, MMS Newsletter Editor
Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
495 Borlaug Hall, St. Paul, MN 55108 (612)625-8249
612-625-9728 FAX, giba-at-puccini.crl.umn.edu

"MICROSCOPY & MICROANALYSIS 96" in Minneapolis, Minnesota, Aug.11-15, 1996





From: Alec Madsen :      alecm-at-u.washington.edu
Date: Mon, 6 Mar 1995 10:48:35 -0800 (PST)
Subject: PC diffraction programs

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X-Sender: alecm-at-homer11.u.washington.edu

Folks,
I'm aware of several programs for the Mac that simulate
diffraction patterns and stereographic projections, etc. How about
similar programs for the PC? Thanks in advance.

Alec Madsen
alecm-at-u.washington.edu
Seattle, Wa




From: jfmjfm-at-umich.edu (John F. Mansfield)
Date: Mon, 06 Mar 1995 15:21:18 -0500
Subject: Wall Street Journal: Thermo buys Fisons

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Message-Id: {199503062018.PAA14966-at-totalrecall.rs.itd.umich.edu}

Well it may not sopund exciting to microscopists until you note that it
tranlates into
Noran's parent company buys Kevex's parent company!
March 3rd issue of Wall Street Journal. Thermo Instrument Systems to
acquire Fisons PLC's scientific instrument division. Cost $320million!
Anyone know whether this covers VG too?

Just thought you guys might like to know.

--
John Mansfield
North Campus Electron Microbeam Analysis Laboratory
413 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (313)936-3352 FAX (313)936-3352
jfmjfm-at-engin.umich.edu, John.F.Mansfield-at-umich.edu
or jfmjfm-at-umich.edu they all reach me!
URL: http://www.engin.umich.edu/~jfmjfm/jfmjfm.html




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Mon, 6 Mar 1995 14:38:46 -0600 (CST)
Subject: PC Programs for Diffraction

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There are at least 1 or 2 old diffraction programs
for the PC in the EMMPDL library. You may access
it by Anonymous FTP at:

WWW.AMC.ANL.GOV

Username=Anonymous
Password=Your Email Address..


Nestor
Your Friendly Neighborhood SysOp.............




From: John Phelps :      phelps-at-ENH.NIST.GOV
Date: Mon, 06 Mar 1995 14:37:36 -0700 (MST)
Subject: terminal emulators

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Hi,
I know this is a little of the subject, but can anyone suggest a ftp site
that has terminal emulators. We need to emulate a tektronics 4205 terminal
to use our new xrd software. Any suggestions would be appreciated.

thanks,
John

John Phelps
NIST - Boulder, CO
303-497-7570




From: Fred Hayes :      fahayes-at-ucdavis.edu
Date: Mon, 6 Mar 1995 13:34:13 -0800 (PST)
Subject: used equipment

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I am looking for the following pieces of used equipment either in good
working order, fair condition and/or in need of minor repair:

1) glass knife breaker for LM microtomy
2) embedding oven (35-80C)
3) balance (top loading or mechanical)

Contact:

Fred A. Hayes 916-752-7712 work
University of California,Davis 916-752-4701 work
School of Medicine
Department ofMedical Pathology; EM Lab
MSIA E-mail:
Davis, CA 95616 fahayes-at-ucdavis.edu

1320 Dogwood Court 916-678-6280 home
Dixon, CA 95620-3227







From: John Phelps :      phelps-at-ENH.NIST.GOV
Date: Mon, 06 Mar 1995 15:44:30 -0700 (MST)
Subject: terminal emulators

Contents Retrieved from Microscopy Listserver Archives
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Hi,
I know this is a little off the subject, but can anyone suggest a ftp site
that has terminal emulators. We need to emulate a tektronics 4205 terminal
to use our new xrd software. Any suggestions would be appreciated.

thanks,
John

John Phelps
NIST - Boulder, CO
303-497-7570




From: gwerdos-at-gnv.ifas.ufl.edu (Greg Erdos ICBR EM Core Lab)
Date: Mon, 06 Mar 1995 10:30:32 -0500 (EST)
Subject: Re: obj. lens 100C replacement

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Microscopy Mail {Microscopy-at-aaem.amc.anl.gov}
Message-id: {01HNT9F975CY93NYWI-at-gnv.ifas.ufl.edu}
MIME-version: 1.0
X-Mailer: Windows Eudora Version 1.4.4
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Content-transfer-encoding: 7BIT

At 08:26 AM 3/3/95 +0100, NICK SCHRYVERS wrote:
} REGARDING obj. lens 100C replacement
}
} The objective lens and the upper and lower column parts of our 20 year old
} side entry JEOL 100 C microscope need replacement. New parts, however, are
} rather expensive in view of the age of the instrument so we're looking for
} anyone who has some second hand spare parts for sale. Sincerely,
} Nick Schryvers tel: 32-3-2180247 fax: 32-3-2180257
} EMAT, University of Antwerp
} Groenenborgerlaan 171
} B-2020 ANTWERP
} Belgium
**********************************************************
There is a JEOL 100 C that may be headed for the scrap heap. They
might be willing to give you parts. Contact Dr. Henry Aldrich, P.O. Box
110700, University of Florida, Gainesville, FL 32611 USA FAX 904-392-5922
(no e-mail) or his department chairman, Edward Hoffmann, at the same
address and FAX number who gets E-mail at EMH-at-GNV.IFAS.UFL.EDU


Good Luck
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Greg Erdos Phone: 904-392-1295
Scientific Director, ICBR EMCL
218 Carr Hall Fax 904-846-0251
University of Florida E-mail: gwerdos-at-gnv,ifas.ufl.edu
Gainesville, FL 32611





From: HUANGY-at-PHYAST.LA.ASU.EDU
Date: Mon, 6 Mar 1995 17:15:27 -0700 (MST)
Subject: change address

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please change my address to yi_huang-at-qmgate.anl.gov. I'd like to continue the
subscription at the new address. Thank you.
Yi Huang
Argonne National Lab
building 212/c221
building 212/c221




From: mrdunlap-at-ucdavis.edu (Michael Dunlap)
Date: Mon, 6 Mar 1995 16:57:54 -0800
Subject: RE:Thermo buys Fisons

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X-Sender: szmdunla-at-bullwinkle.ucdavis.edu
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John-

All instruments divisions of fisons are going to Thermo Instrument Systems
including VG.

Just what we all needed. I sure service and parts are going to be even
easier to get.

Mike

---------------------------------------------------------------------------
| Michael Dunlap | lab (916) 752-0284 |
| Facility For Advanced Instrumentation | fax (510) 422-2282 |
| University of California | mrdunlap-at-ucdavis.edu |
| Davis CA, 95616 | |
===========================================================================






From: W.L. Steffens :      STEFFENS.B-at-calc.vet.uga.edu
Date: Mon, 6 Mar 1995 10:46:32 EST
Subject: TEM of Cell Culture

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To Christine Powers,

We have successfully used LR White resin for embedment of monolayers
grown in Leighton tubes. The problems encountered were the same ones you
encounter when using LR White in open molds or silicone rubber molds, or
just about anything other than gelatin capsules. It's most convenient to
embed coverslips in flat, open molds such as the silicone rubber type. We
had such problems with this (ie excluding oxygen, penetration of the molds
by the resin, etc) that we began embedding the coverslips standing upright
in gelatin capsules. The long narrow coverslips of the Leighton tubes are
just accommodated by a 00 gelatin capsule. This neccessitates
considerably more hand trimming to get to the monolayer profile, but
embedding problems are eliminated.
I might also add, that once the block face is prepared for cutting,
the exposed coverslip may be peeled away from the embedded cells, greatly
facilitating cutting. If you do this, I would also recommend mounting the
sections on a support filmed grid, as the cells will be on the edge of the
section.
Good Luck!

-=W.L. Steffens=-
College of Veterinary Medicine
University of Georgia




From: Software department :      software-at-oimag.win-uk.net
Date: Mon, 06 Mar 1995 17:46:23
Subject: Job Opportunities/Microanalysis Software

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X-Mailer: WinNET Mail, v2.30
Message-ID: {463-at-oimag.win-uk.net}
Reply-To: Software department {software-at-oimag.win-uk.net}
To: microscopy-at-aaem.amc.anl.gov

The following advertisement has recently been placed in the press
and may be of interest to those who read this Newsgroup who would
like to work in the UK:

SOFTWARE DEVELOPMENT FOR MICROANALYSIS
The Software team at Oxford Instruments Microanalysis Group develop
high quality instrumentation products to satisfy customers in an
international marketplace. Currently we have a number of vacancies
for individuals who would enjoy the challenge of solving complex
problems to generate leading edge products. If you are competent in
Visual Basic, C++ or C, have a basic understanding of electronics and
computer interfacing and feel you could make a strong contribution in
any or all of the following areas please contact us:

Practical applications experience using an SEM or microprobe.
Knowledge of x-ray microanalysis and familiarity with operation of WD spectrometers.
Experience in project management and identifying the "voice of the customer".
Practical use of applied mathematics, numerical methods, chemometrics.
Experience with real time control and hardware automation.

Successful applicants will receive an attractive benefits package
and salary commensurate with capability.

Please send a copy of your CV, and a telephone contact number to

Peter Statham ,
Oxford Instruments Microanalysis Group
Halifax Road, High Wycombe, Bucks HP12 3SE England
Telephone (01494) 442255 Fax (01494) 461033
email: software-at-oimag.win-uk.net


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From: jfmjfm-at-umich.edu (John F. Mansfield)
Date: Mon, 6 Mar 1995 14:41:40 -0500
Subject: Job Posting

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X-Sender: jfmjfm-at-srvr5.engin.umich.edu
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The following is a copy of the job posting that recently appeared on the
University of Michigan Gopher Server UM GopherBlue.

The full URL is:
gopher://gopher.itd.umich.edu:8888/00/acadaff/hris/gopher/Job%20Postings/Pro
fessional%5CAdministrative/-at-39-at-RES%20ASSOC%20II%20ENGR%20%28Materials%20Scie
nce%20%26%20Engineering%29


Job Title: RES ASSOC II ENGR Grade: 09 Min/Max $ 25,500/ 64,500
Posting Number: T-95-0826-LM Materials Science & Engineering
Open Date: 03/06/1995 Close Date: 03/10/1995
Jobclass: 12871 Hours: 40.00

DUTIES:
Operate and train users in the use of the lab instruments: a JEOL
200FX Analytical Electron Microscope, a JOEL 4000EX High Resolution
electron Microscope, a Philips EM420 Transmission Electron Microscope,
an ElectroScan E3 Environmental Scanning Electron Microscope, a
Digital Instruments Scanning Force Microscope, a PHI 5400 X-ray
Photoelectron Spectrometer and a PHI 600 Scanning Auger Microprobe;
the successful candidate will not necessarily need to be proficient in
the use of all instruments specified, but familiarity with at least
four is essential; other duties include: aiding users with reduction
and analysis of data recorded on lab instruments; aiding users with
sample preparation equipment and preparing samples; help other lab
staff maintain lab equipment; maintain lab darkroom and supplies; take
part in collaborative projects with other members of the University;
develop personal research projects as time permits.

DESIRED QUALIFICATIONS:
Knowledge of Analytical Electron Microscopy, Scanning Transmission
Electron Microscopy and High Resolution Electron Microscopy; TEM
expertise should include detailed understanding of diffraction
contract, defect imaging, selected area diffraction, high resolution
imaging, high resolution image simulation and analysis; experience
with computer programming in FORTRAN, C or Pascal.

MINIMUM QUALIFICATIONS:
Master's degree or equivalent in a scientific related field;
demonstrated prior related work experience; familiarity with computer
systems such as Apple Macintoshes, PCs and/or UNIX workstations;
working knowledge of general transmission electron microscopy and
scanning electron microscopy; experience with standard data analysis
for analytical techniques such as X-ray energy dispersive spectroscopy
(XEDS), electron energy loss spectroscopy (EELS) and micro
diffraction; good interpersonal skills.


---------------------------------------------------------------------------

Present Applications For This Position To The Following Office:

Ann Arbor Campus Employment Services Office
Room G250 Wolverine Tower
3003 South State Street
Ann Arbor, Michigan 48l09

(313) 764-6580

8 AM to 5 PM, Monday - Friday



John Mansfield
North Campus Electron Microbeam Analysis Laboratory
413 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (313)936-3352 FAX (313)936-3352
jfmjfm-at-engin.umich.edu, John.F.Mansfield-at-umich.edu
or jfmjfm-at-umich.edu they all reach me!
URL: http://www.engin.umich.edu/~jfmjfm/jfmjfm.html






From: desclinj-at-ulb.ac.be (Desclin Jean)
Date: Tue, 7 Mar 1995 11:00:54 +0100 (MET)
Subject: testis fixation

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Hello Barbara!
I am afraid that, under the conditions you describe for fixation,
significant shrinkage should be unavoidable whatever fixative
would be used.
I already mentioned that picric acid 'per se' in the fixing fluid
does not ensure adequate preservation of acrosomial structure which
is required for easy classification of spermatids (and thus of
stages of the seminiferous epithelium.)
What should be avoided in any case is the presence of acids usually
part of most recipes for fixatives, such as acetic acid and the like.
If you use '10 percent formalin', you may experience some problems
because you in fact never know the actual composition of such
solution: it polymerizes on the shelf and becomes also quite acidic!
You should prepare a solution of phosphate buffered (pH 7.2) 4 percent
formaldehyde from paraformaldehyde powder on the same day (or the
previous one) that you are sacrificing the animals (you should find
recipes for making such solution in any practical handbook for
microscopical technique ;-)).

Fixation through immersion of the testes in the fixative may be
almost acceptable for mice testes because they are small; this won't
be the case for other species, even for the rat. You may try to make
small (with caution!) incisions in the albuginea with a very sharp
razor blade when the testes have stayed for at least an hour in the
fixative, in order to improve penetration of the formaldehyde.
Another trick to improve diffusion and fixation is to add from 0.5 to
1.0 percent (in volume) of DMSO (dimethylsulfoxide) to the fixing
fluid (best is to combine both...)

BUT,LAST and PROBABLY _MOST_ IMPORTANT: you should realize that
formaldehyde fixation, i.e. crosslinking, proceeds exceedingly
slowly! Therefore, tissues should stay in the fixative at least
two weeks (one year or more won't harm!) before being further
processed (two weeks probably is a conservative estimate). Assessment
of pathological tissues fixed for only a few hours in NBF may
frequently be required for practical reasons, but don't expect such
methods to result in nice preservation of testis tissue, especially
if you need estimating quality of spermatogenesis!

You also may try to enhance fixation - and shorten the duration
required for fixation - by performing it in the microwave oven: several
cycles of no more than one minute at a time (perhaps 10 times, tissue
temperatures exceeding 50 degrees celsius should be avoided), if
you are indeed in a hurry and can't wait several weeks for
fixation to proceed at its usual pace. If you try this, don't forget
to place a water load (a becher container with about 750-1000 ml
of water) besides your fixative containing flask in the oven.

That's all what I can think of. HTH
Good luck
John


***********************************************************
* Jean C. Desclin (John), Associate Prof. of Histology *
* Laboratory of Histology - Faculty of Medicine *
* Brussels Free University (U.L.B.) *
* e-mail: desclinj-at-ulb.ac.be (internet) *
* snail mail: route de Lennik 808 *
* B - 1070 Brussels Belgium *
***********************************************************




From: gwerdos-at-gnv.ifas.ufl.edu (Greg Erdos ICBR EM Core Lab)
Date: Tue, 07 Mar 1995 09:38:54 -0500 (EST)
Subject: JEOL 100 C parts

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Regarding the following message:

I was unable to reach Nick Schryvers at his E-mail address therefore
my reply is below. Perhaps others will be interested:

Nick writes:
} } } The objective lens and the upper and lower column parts of our 20 year old
} } } side entry JEOL 100 C microscope need replacement. New parts, however, are
} } } rather expensive in view of the age of the instrument so we're looking for
} } } anyone who has some second hand spare parts for sale. Sincerely,
} } } Nick Schryvers tel: 32-3-2180247 fax: 32-3-2180257
} } } EMAT, University of Antwerp
} } } Groenenborgerlaan 171
} } } B-2020 ANTWERP
} } } Belgium
} } **********************************************************

} } My reply:

There is a JEOL 100 C that may be headed for the scrap heap. They
} } might be willing to give you parts. Contact Dr. Henry Aldrich, P.O. Box
} } 110700, University of Florida, Gainesville, FL 32611 USA FAX 904-392-5922
} } (no e-mail) or his department chairman, Edward Hoffmann, at the same
} } address and FAX number who gets E-mail at EMH-at-GNV.IFAS.UFL.EDU
} }
} }
} } Good Luck
} } -at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
} } Greg Erdos Phone: 904-392-1295
} } Scientific Director, ICBR EMCL
} } 218 Carr Hall Fax 904-846-0251
} } University of Florida E-mail: gwerdos-at-gnv,ifas.ufl.edu
} } Gainesville, FL 32611
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Greg Erdos Phone: 904-392-1295
Scientific Director, ICBR EMCL
218 Carr Hall Fax 904-846-0251
University of Florida E-mail: gwerdos-at-gnv,ifas.ufl.edu
Gainesville, FL 32611





From: Jay Jerome :      jjerome-at-isnet.is.wfu.edu
Date: Tue, 7 Mar 1995 10:38:23 -0500 (EST)
Subject: Re: testis fixation

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I concur with Dr. Desclin's suggestions- however, I would add that pH and
osmolarity of your solutions is easy to do, and can be helpful.
Perfusion fixation of the testis is also a possibility. Those are my
humble additions-


Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-isnet.is.wfu.edu *
**************************************************************

On Tue, 7 Mar 1995, Desclin Jean wrote:

}
}
} Hello Barbara!
} I am afraid that, under the conditions you describe for fixation,
} significant shrinkage should be unavoidable whatever fixative
} would be used.
} I already mentioned that picric acid 'per se' in the fixing fluid
} does not ensure adequate preservation of acrosomial structure which
} is required for easy classification of spermatids (and thus of
} stages of the seminiferous epithelium.)
} What should be avoided in any case is the presence of acids usually
} part of most recipes for fixatives, such as acetic acid and the like.
} If you use '10 percent formalin', you may experience some problems
} because you in fact never know the actual composition of such
} solution: it polymerizes on the shelf and becomes also quite acidic!
} You should prepare a solution of phosphate buffered (pH 7.2) 4 percent
} formaldehyde from paraformaldehyde powder on the same day (or the
} previous one) that you are sacrificing the animals (you should find
} recipes for making such solution in any practical handbook for
} microscopical technique ;-)).
}
} Fixation through immersion of the testes in the fixative may be
} almost acceptable for mice testes because they are small; this won't
} be the case for other species, even for the rat. You may try to make
} small (with caution!) incisions in the albuginea with a very sharp
} razor blade when the testes have stayed for at least an hour in the
} fixative, in order to improve penetration of the formaldehyde.
} Another trick to improve diffusion and fixation is to add from 0.5 to
} 1.0 percent (in volume) of DMSO (dimethylsulfoxide) to the fixing
} fluid (best is to combine both...)
}
} BUT,LAST and PROBABLY _MOST_ IMPORTANT: you should realize that
} formaldehyde fixation, i.e. crosslinking, proceeds exceedingly
} slowly! Therefore, tissues should stay in the fixative at least
} two weeks (one year or more won't harm!) before being further
} processed (two weeks probably is a conservative estimate). Assessment
} of pathological tissues fixed for only a few hours in NBF may
} frequently be required for practical reasons, but don't expect such
} methods to result in nice preservation of testis tissue, especially
} if you need estimating quality of spermatogenesis!
}
} You also may try to enhance fixation - and shorten the duration
} required for fixation - by performing it in the microwave oven: several
} cycles of no more than one minute at a time (perhaps 10 times, tissue
} temperatures exceeding 50 degrees celsius should be avoided), if
} you are indeed in a hurry and can't wait several weeks for
} fixation to proceed at its usual pace. If you try this, don't forget
} to place a water load (a becher container with about 750-1000 ml
} of water) besides your fixative containing flask in the oven.
}
} That's all what I can think of. HTH
} Good luck
} John
}
}
} ***********************************************************
} * Jean C. Desclin (John), Associate Prof. of Histology *
} * Laboratory of Histology - Faculty of Medicine *
} * Brussels Free University (U.L.B.) *
} * e-mail: desclinj-at-ulb.ac.be (internet) *
} * snail mail: route de Lennik 808 *
} * B - 1070 Brussels Belgium *
} ***********************************************************
}




From: Joyce Craig :      bafpjec-at-uxa.ecn.bgu.edu
Date: Tue, 7 Mar 1995 11:12:26 -0600 (CST)
Subject: Re: Epoxy Resins: Accelerator?

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Dennis Shubitowski {dennis-at-odin.morph.med.umich.edu}
In-Reply-To: {Pine.3.03.9503021552.B11478-b100000-at-odin}
Message-Id: {Pine.3.89.9503071104.A27101-0100000-at-ecom3.ecn.bgu.edu}
Mime-Version: 1.0
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On Thu, 2 Mar 1995, Dennis Shubitowski wrote:

} One of the divisions of our lab teases apart sural nerve tissue that have
} been kept in a 1:1 mixture of epon:propylene oxide. The samples are very
} easy to tease when the mixture is very fresh. The problem is that samples
} became back-logged and were not able to be teased right away. A quick
} decision had to be made, and the samples were frozen at -70C until they
} could be teased.
}
} The nerve fibers need to be infiltrated to be teased but not polymerized
} or else they become too fragile. A problem is becoming evident that after a
} long period of storage, the nerve samples are being fully infiltrated
} and polymerized despite being frozen (which we thought would almost stop
} the polymerization process because it is largely dependant on heat).
} Realizing that hind-sight is 20/20, would it have been better to leave the
} accelerator out of the epoxy mixture? What affect would this have on the
} quality of the resin? I understand accelerator to act as a catalyst being
} a part of the polymerization reaction but not really consumed. Any
} opinions to offer?
}
} Thanks,
}
} Dennis
}
Why do you store the fibers in resin? Isn't teasing them very messy? I
teased sural nerve fibers when
working for a neuropathologist. I fixed them and stored in buffer.
Also I sometimes used collagenase, which made the teasing easier. } } }
}




From: Laurie Frederick :      frederick_laurie-at-vanlab.paprican.ca
Date: Tue, 7 Mar 1995 09:20:30 PDT
Subject: Re: Image Analysis Systems

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Message-Id: {MAILQUEUE-101.950307092030.384-at-vanlab.paprican.ca}
To: "Griffin, Robin" {rgriffin-at-eng.uab.edu} , microscopy-at-aaem.amc.anl.gov

Hello,
I have information on an "Imagist" image analysis system from
Princeton Gamma Tech (PGT) which seems to be finding quite a bit of
popularity. I have come across it several times in speaking to users
during my hunt for an EDX system.
Imagist runs on a sparc station.
PGT in New Jersey, USA , can be reached at (609) 924-7310.

I have not used this system I have only seen it in use at the university
here.
Goodluck,
Laurie


On 1 Mar, 1995 Robin Griffin wrote:
}
} I'm interested in finding out what the most commonly used image
analysis
} systems for light microscopy, materials applications. I am aware of
} home-built, Beuler, Leco, and Kontron/Ibas. What else is out there
and what
} is used most in both University and industrial settings?
}
} Thanks for your help.
}
}

______________________________________________________________________
Laurie Frederick, A.SC.T. PAPRICAN
Corrosion Control Group 3800 Wesbrook Mall
The Pulp and Paper Research Vancouver, B.C.
Institute of Canada Canada V6S 2L9

Email: frederick_laurie-at-vanlab.paprican.ca
Tel: 604-222-3200 Fax: 604-222-3207




From: gwerdos-at-gnv.ifas.ufl.edu (Greg Erdos ICBR EM Core Lab)
Date: Tue, 07 Mar 1995 14:21:43 -0500 (EST)
Subject: Negative scanner

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We are considering purchasing a scanner for TEM negatives.

1. Is this a good idea?

2. Is there a consensus on which one to buy?

Any and all input appreciated.
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Greg Erdos Phone: 904-392-1295
Scientific Director, ICBR EMCL
218 Carr Hall Fax 904-846-0251
University of Florida E-mail: gwerdos-at-gnv,ifas.ufl.edu
Gainesville, FL 32611





From: meh-at-aretha.jax.org (Margaret Hogan)
Date: Tue, 07 Mar 1995 15:38:40 -0500
Subject: job openning

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Message-Id: {199503072047.PAA10944-at-aretha.jax.org}
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Histology - Biomedical Technologist/ Senior Biomedical Technologist

A regular full time position is open in The Jackson Laboratory Biological
Imaging Department - Histology Laboratory. The Jackson Laboratory is a
non-profit independent laboratory founded in 1929 on the premise that the
causes of cancer and other diseases could be discovered through
Mammalian genetic research. The Laboratory specializes in mammalian
genetics using inbred laboratory mice as model systems to study health
problems such as cancer diabetes, anemia, heart disease and aging.
Located on a large island in the gulf of Maine and surrounded by Acadia
National Park, The Jackson Laboratory is currently undergoing a major
expansion of its scientific staff and its research facilities.

Applicants with a Bachelors degree and two years related
laboratory experience working with Murine specimens preferred. The
position includes routine histological techniques ie paraffin embedding,
single and serial sectioning and cryotomy in conjunction with
Immunohistochemistry techniques, staining using heavy metals as well as
other special stains.

The successful candidate must be a self starter, pay attention to detail
and be able to work independently with little supervision.
This individual will be responsible for providing services in support of
numerous diverse research projects, must interact well with multiple
users and work productively in a team environment. The position includes
opportunities for advancement.

Salary range is mid to high $20,000 plus benefits and is negotiable
depending on level of experience.

Interested applicants should send CV to:

Joanne Bradt
Employment Specialist
The Jackson Laboratory
600 Main Street
Bar Harbor Maine 04609
(207) 288-3371 ext. 1281
(207) 288-3371 ext. 1082 FAX
jcb-at-aretha.jax.org





From: tivol-at-tethys.ph.albany.edu
Date: Tue, 07 Mar 1995 16:08:09 EST
Subject: Re: Negative scanner

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Dear Greg,
There are at least two types of scanner to consider depending on your
application. For scanning images of the usual type with many gray levels, a
CCD array will give excellent results and is very fast; however, for quantita-
ting ED patterns, nothing beats a scanner with a small illuminating spot. The
problem with a CCD array for ED is that the highest intensities are in areas
with very low transmission surrounded by areas with high transmission, so in-
ternal reflections in the CCD lens, etc. will give erronious values for the
intensities. Any of the high-resolution CCD arrays will work for images, since
the changes in contrast are not so abrupt. Perkin-Elmer and Optronics are two
names for small-illuminating-spot scanners, and doubtless there are others.
We had an Eikonix (linear CCD array) at one time, but were not happy with it.
Good luck.
Yours,
Bill Tivol




From: Richard E. Edelmann :      EDELMARE-at-CASMAIL.MUOHIO.EDU
Date: Tue, 7 Mar 1995 16:42:53 -500
Subject: Polaroid Films

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Here's a little information that I just received from polaroid
that should be of interest to the microscopy community. I'd be
interested in hearing any comments anyone (particularly any Polaroid
reps.) might care to share with the BBS.

Recently we have been noticing that the type 55 film we are
purchasing was arriving with a relatively short period before
expiration. We were questioning if our supplier might have trying to
unload older film on us. So I just called up Polaroid an asked them
what their lead time from manufacture to expiration date was, and I
was informed that for type 55 and type 665 (B&W P/N film) the
expiration lead time is only 9 months.

If you allow say 2-5 months from manufacture to shipping to
distributors to shipping to users (which seems very reasonable for
such commercial retail) this leaves only 4-7 months before
expiration! This short of an expiration date does not lend itself at
all to buying in larger quantities in order to obtain any sort of a
price break.

I do not know how much extension can be obtained by storage at say
4 C (May be someone out there does know). But we seem to start
having problems with film only a couple of months after the
expiration date even after cold storage for a few months.

You might want to consider this short time to expiration before
you go and stock up on polaroid film.


Richard E. Edelmann
Electron Microscopy Facility Supervisor
Miami University, Oxford, OH 45056
Ph: 513-529-5712
E-mail: edelmare-at-muohio.edu




From: John E. Johnson, Jr. :      76055.2216-at-compuserve.com
Date: 07 Mar 95 17:05:15 EST
Subject: Referees for Microscopy Research and Technique

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Although the journal Microscopy Research and Technique has a large editorial
board, we cannot cover every research area that involves microscopy. Therefore,
I would like to invite those of you who are interested in serving as ad hoc
referees for articles submitted to the journal to send, by e-mail, your name,
mailing (regular mail) address, phone number, fax number, and research interests
using key words such as biology, materials science, pathology, microanalysis,
stereology, magnetic domain, ceramics, immunocytochemistry, kidney, etc.

John E. Johnson, Jr., Ph.D.
Editor-in-Chief, Microscopy Research and Technique






From: gwerdos
Date: Tuesday, March 07, 1995 2:21PM
Subject: Negative scanner

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We are considering purchasing a scanner for TEM negatives.

1. Is this a good idea?

2. Is there a consensus on which one to buy?

Any and all input appreciated.
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Greg Erdos Phone: 904-392-1295
Scientific Director, ICBR EMCL
218 Carr Hall Fax 904-846-0251
University of Florida E-mail: gwerdos-at-gnv,ifas.ufl.edu
Gainesville, FL 32611





From: charlesworth.jon-at-mayo.EDU (Jon Charlesworth)
Date: Tue, 7 Mar 1995 16:10:54 +0200
Subject: Tissue Processors

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Ed,
We have had 3 different tissue processors in the past 6 years, 3
LKB's, one Lynx, and 3 RMC's model 4189. LKB no longer manufactures a
system so I will forego 'ripping' their machine. We purchased a Lynx
system about four years ago. We found the same problem that W.L. Steffens
had with the QC of the expendibles. We lost tissue due to improper sealing
of the specimen chambers. Also the side 'openings' are relatively large
and we therefore do not run tissue smaller than 0.5 mm3 in these holders.
Extreme care must be used when removing tissue from the holders because
tissue has a tendency to adhere to the lid of the holder making it possible
to mix tissues when using the multiwell chambers. The unit itself though
has been extremely reliable.
About 10 months ago we purchased 3 RMC processors. These have
proven not to be as reliable as the Lynx (we have had temperature and some
programming problems). We therefore have had the opportunity to test RMC's
service department which to date has been able to get us up and running
with minimal down time. The big advantage of the RMC units are the
specimen chambers which seal better and hold smaller pieces of tissue.
Currently we use only the RMC units for tissue processing
(approximately 3000 samples annually). The Lynx system has been modified
and is used 3-4 days (and evenings) a week to immunolabel grids for our IEM
program. Hope this helps.

{jon charlesworth}
Electron Microscopy Facility
Mayo Clinic







From: gwerdos
Date: Tuesday, March 07, 1995 2:21PM
Subject: Negative scanner

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are considering purchasing a scanner for TEM negatives.

1. Is this a good idea?

2. Is there a consensus on which one to buy?

Any and all input appreciated.
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Greg Erdos Phone: 904-392-1295
Scientific Director, ICBR EMCL
218 Carr Hall Fax 904-846-0251
University of Florida E-mail: gwerdos-at-gnv,ifas.ufl.edu
Gainesville, FL 32611





From: fskarl-at-goodyear.com (Frank Karl)
Date: Wed, 8 Mar 1995 08:19:08 -0500
Subject: Long term storage of Polaroid film

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} noticing that the type 55 film ... with a relatively short period before
} expiration
}
} But we seem to start
} having problems with film only a couple of months after the
} expiration date even after cold storage for a few months.

Please forgive the above condenstation :-}

For the last 15 years we have stored Polaroid type 55 film in a freezer
without harm. This appears to negate the expiration date. We usually
allow the film to thaw out completely before opening the sealed package,
but sometime this process is rushed a bit and then, more often than not we
have problems.


Frank Karl fskarl-at-goodyear.com
Goodyear Tire & Rubber Co. Voice 216.796.7818
Analytical Services - Dept 415B Fax 216.796.3304
142 Goodyear Blvd
Akron, OH 44305
U.S.A.









From: Michael Boucher :      BOUCHER-at-tcrca.usbm.gov
Date: Wed, 8 Mar 1995 09:12:42 CST
Subject: Re: Polaroid Films

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We have found that to be the situation for years, but we order it by
the case and refrigerate it (not freezing). It seems to be good for
at least a year past expiration and we have had some film that was
several years old still work. I suspect that there could be some
degradation, but for our routine SEM and light microscopy, it hasn't
been a problem. Maybe Polaroid has done some testing?

*****************************************************************
Michael L. Boucher Sr. Boucher-at-TCRCA.USBM.GOV
Geology-Mineralogy/Chemistry Labs Ph 612-725-4614
Twin Cities Research Center Fax 612-725-4527
U.S. Bureau of Mines Center 725-4500
Department of Interior
5629 Minnehaha Avenue South
Minneapolis, MN 55417-3099
U.S.A.
*****************************************************************





From: chswartz-at-MIT.EDU
Date: Wed, 08 Mar 1995 11:06:43 EST
Subject: ultrathinsectioning material containing quartz

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I am going to be attempting to thin section sediment samples containing
quartz and want the thin sections to be approx. 50-70nm in thickness for TEM
analysis. I have to buy a diamond knife and would much appreciate input on the
blade angle to choose. A representative at Polysciences told me I should go to
a 55 degree knife instead of a 45, but I was wondering if I would be sacrificing
much of the ability to cut sections down to 50nm. The quartz grains are approx.
200 um in diameter. Is there a particular manufacturer I should get the knife
from? What is an appropriate cutting speed? Can I expect the knife to last
only a VERY short time? I would appreciate any suggestions and advice. My
address is:
chswartz-at-MIT.edu

THANKS




From: jerry-at-biochem.dental.upenn.edu
Date: Wed, 8 Mar 1995 12:03:50 -0500
Subject: Carbon Putty

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Does anyone on the listserver know where (prefereably in the U.S.)
that I can get Carbon Putty. I'm down to the last of what I had that
someone picked up at a conference in Germany a few years ago. It is
basically a mixture of some kind of soft wax and carbon and is a very good
specimen mounting material since there is no drying or out-gassing. Ted
Pella doesn't seem to have it so maybe someone out there knows about this
product and can save me a hunt.

Thanks---Jerry





From: ard-at-atom.ansto.gov.au (Arthur Day)
Date: Thu, 9 Mar 1995 04:22:35 +1100
Subject: Re: Polaroid Films

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} I do not know how much extension can be obtained by storage at say
} 4 C (May be someone out there does know). But we seem to start
} having problems with film only a couple of months after the
} expiration date even after cold storage for a few months.
}
} Richard E. Edelmann

We are just finishing our last few boxes of September 1994 Type 55 film
(stored in a refrigerator). It still seems to be OK.


Arthur Day, Electron Microscopy Group
Ansto Advanced Materials Program Phone: 61-2-717-3457
PMB 1, Menai (Sydney), NSW, 2234 Fax: 61-2-543-7179
Australia
Email: ard-at-atom.ansto.gov.au







From: Eric Kokko :      kokko-at-EM.AGR.CA
Date: Wed, 08 Mar 1995 15:30:52 -0500
Subject: Tracor 8502 users, help: Image convert to tiff

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Message-Id: {sf5dcd2b.022-at-EM.AGR.CA}
X-Mailer: Novell GroupWise 4.1


------------------- tn8502.asc follows --------------------
Tracor 8502 users: Help with image convert to tiff

We operate a Tracor Northern (Noran) 8502 Image Analyzer and have
been trying to export images to a VAX via KERMIT with no success.
Has anyone done this sort of image file conversion and transfer
successfully?

Background: The Tracor software can convert an image file from
TN's format (.IMG) to a .TIF file (type 4, version 42). We can
successfully transfer this TIFF image file, via KERMIT, to or VAX
(ie. the file appears and has the appropriate size). When we try
to open this TIFF image (using a PC on our LAN), we always fail
regardless of the software we have employed (e.g. with Photoshop,
HiJack Pro).

If you have any ideas, please contact. I have lots of additional
contextual information to add to this. Thank you!

Eric Kokko
Electron Microscopy and Image Analysis
Agriculture and Agri-Food Canada
Lethbridge Research Centre
P.O. Box 3000,
Lethbridge, Alberta
CANADA T1J 4B1

Phone 403-327-4591 (Voice 367)
FAX 403-382-3156
INTERNET kokko-at-em.agr.ca





From: jerry-at-biochem.dental.upenn.edu
Date: Wed, 8 Mar 1995 15:47:44 -0500
Subject: Carbon Putty

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To those interested in Carbon Putty,

Someone has just given me all you could ever want to know about this
product. It is available from:
Bal-Tec Products, Inc.
984 Southford Road
P.O. Box 1221
Middlebury, CT 06762; (800) 875-3713, FAX (203) 598-3658

It is called "Leit-C-Plast; Cat. # B 801014075
Current price is $70.00 for 15g

Other properties:
-high electric conductivity
-long-life plasticity
-high vacuum resistance
-sufficient adhesive power
-low sample contamination
-no disturbing ED-x-ray lines
-Resistance R~100 kOhm mm2/m
Thanks for saving me the hunt---Jerry





From: M. ROOKS :      rooks-at-nnf.cornell.edu
Date: Wed, 08 Mar 1995 17:59:37 EST
Subject: carbon putty

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"Mung II" is not carbon putty, but is very much like it: electrically
conducting, a good thermal conductor, and very low vapor pressure.
We use Mung in our ion mill, but in the SEMs most people prefer clips
or carbon tape. You can buy mung from

Comonwealth Scientific Corp.
500 Pendleton St.
Alexandria, VA 22314
703-548-0800

$160 for 5 gr.



_______________________________________________________________
Michael Rooks 607-255-2329 voice
National Nanofabrication Facility 607-255-8601 fax
at Cornell University rooks-at-nnf.cornell.edu
Knight Laboratory
Ithaca, NY 14853 USA

__ __ __ __ ________ National
____ __ ____ __ __ Nanofabrication
__ ____ __ ____ _____ Facility
__ __ __ __ __ Cornell University

_______________________________________________________________






_______________________________________________________________
Michael Rooks 607-255-2329 voice
National Nanofabrication Facility 607-255-8601 fax
at Cornell University rooks-at-nnf.cornell.edu
Knight Laboratory
Ithaca, NY 14853 USA

__ __ __ __ ________ National
____ __ ____ __ __ Nanofabrication
__ ____ __ ____ _____ Facility
__ __ __ __ __ Cornell University

_______________________________________________________________








From: Charles A. Garber
Date: Thu, 09 Mar 1995 01:36:29 EST
Subject: "Carbon putty"

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To: Jerry-at-biochem.dental.upenn.edu

SPI Supplies

RE: Question about "carbon putty"

You are asking about a product called "Leit-C-Plast", which is found on
page 46 of the current SPI Supplies "SourceBook" as SPI #5057-AB. It is a
highly conductive adhesive plastic and is designed for mounting large
specimengo a very long way. I hope
this information will be useful to you.


Charles A. Garber, Ph. D.
PRESIDENT
SPI SUPPLIES
PO BOX 656
West Chester, PA 19380

Ph: (800) 242-4SPI
FAX: (610) 436-5755





From: John Millar :      jjmill-at-RMIT.EDU.AU
Date: Thu, 9 Mar 1995 10:38:46 EST-10
Subject: Re: Polaroid Films

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"Richard E. Edelmann" {EDELMARE-at-CASMAIL.MUOHIO.EDU}

Richard Edelmann wrote " I'd be
} interested in hearing any comments anyone (particularly any Polaroid
} reps.) might care to share with the BBS.
}
} Recently we have been noticing that the type 55 film we are
} purchasing was arriving with a relatively short period before
} expiration. We were questioning if our supplier might have trying to
} unload older film on us. So I just called up Polaroid an asked them
} what their lead time from manufacture to expiration date was, and I
} was informed that for type 55 and type 665 (B&W P/N film) the
} expiration lead time is only 9 months. "
}
I have used Polaroid 665,667,55 for nearly 20 years with no problems
of any continuing significance (except maybe cost !!). We have always
purchased in bulk (large boxes of 50 boxes) and stored the material
in a standard refrigerator, not freezing it. Usual practice would be
to allow the boxes to equilibrate at room temperature before use but
sometimes usage rate prevented this. The sensitivity does appear to
change with temperature, but we have nver bothereded to investigate
in detail. In the usual situation, we have not expereinced any
problems and have both negs and prints which are old and still very
good quality.
Cleanliness of the holder and specially the rollers is critical to
performance and they are cleaned after EVERY cartridge is exhausted
and before the next one is in place.
cheers
jjm
Professor John J. Millar
Head, Department of Applied Physics
Electron Microscope Unit
RMIT, GPO Box 2476V, MELBOURNE AUSTRALIA 3001
+613 660 2602 fax 613 660 3837
email jjmill-at-rmit.edu.au




From: Charles A. Garber
Date: Thu, 09 Mar 1995 01:36:53 EST
Subject: Thin sectioning of quartz particles

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To: chswartz-at-MIT.edu

SPI Supplies
West Chester, PA 19380 USA

RE: Ultrathin sectioning material containing quartz

We have had some amount of experience in our own laboratory with the thin
sectioning of these kinds of samples for TEM characterization.

There are the obvious trade offs, that is, th that a) the price is much
cheaper and b) you are using the "normal" narrow angle (e.g. 45 deg.)
knives and therefore you don't have to worry about compression effects.

While the ability to cut sections is somewhat related to the experience of
the one doing the sectioning (and it is also an art), so long as you are
patient, you should be able to do it.

If you wanted to send us one of your samples, we could produce sections for
you, and essentially determine the exact conditions under which you too
could easily get sections. There would be a fee of course for this,
however, you would know for sure, one way or another, whether sections
could or could not be made of your material,and if they could, then you
would also know the exact cutting conditions.

Another important consideration would be the choice of embedding resins and
we would recommend for quartz particles our SPI # 2660 SPI-PON 812 resin
kit since this particular resin seems to be the most user friendly in terms
of polymerizing to a hardness for which sections can be made of the
embedding quartz particles.

You have also asked a question that touches on the expected longevity of
the diamond knife when cutting these kinds of materials. You sort of have
to think about it as an analog to the mileage expected from a pair of
tires. You can buy imported tires or you can buy domestic ones, however,
what really counts is how you drive your car. Be gentle, and the knife will
last longer. Be abusive, and it will have a shorter life time. Obviously,
cutting quartz particles is going to be "hard" on the knife. You can
extend the longevity be making the particles smaller before embedding. You
can get good sections faster, and therefore also less knife wear, by curing
the SPI-Pon 812 resin harder right away, putting less wear and tear on the
knife to get to that point.

I hope this information has been helpful to you. Let me know if you have
any other questions.

Charles A. Garber
SPI Supplies
PO Box 656
West Chester, PA 19380 USA

Ph: (800) 242-4SPI
FAX: (610) 436-5755
e-mail: cgarber-at-cerfnet.com





From: jouneau-at-cime.epfl.ch (Pierre-Henri Jouneau)
Date: Thu, 9 Mar 1995 15:51:13 +0100
Subject: TRINOCULAR JOINT MEETING ON ELECTRON MICROSCOPIES

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***************************************************************************

ANNOUNCEMENT

Trinocular Joint Meeting on Electron Microscopies

Lausanne, June 26-30, 1995

***************************************************************************

The Swiss Society of Optics and Electron Microscopy, the French Society
of Electron Microscopy and the Belgian Society of Microscopy have decided
to hold a joint meeting in 1995. This conference will be held at the
"Ecole Polytechnique Federale de Lausanne" (EPFL), Lausanne, Switzerland,
from June 26 to 30, 1995. It will include two tutorial days and a three-days
conference which will be mainly focused on the recent progresses in TEM and
scanning probe microscopies for materials science and biological fields.


********** SCIENTIFIC PROGRAMME **********

Monday 26 and Tuesday 27
TUTORIALS, with practical training, for groups of 6 to 20 people.

- Cryo-microscopy of vitrified specimens: theory and practice
(Jacques Dubochet)
- Practical aspects of the methodology of image analysis in oncology
(Ricardo Laurini)
- TEM image simulation of crystalline materials (Pierre Stadelmann)
- Electron holography (Conradin Beeli)
- Analytical microscopy with a field emission TEM (Klaus Leifer)
- TEM sample preparation in materials science (Daniele Laub)
- Applications of large angle convergent beam electron diffraction
techniques (LACBED) (Jean-Paul Morniroli)

Evening Tuesday 27
Welcome ceremony and conference opening lecture.

"Perception du monde exterieur par les systemes vivants"
by Yves de Ribaupierre, University of Lausanne.

Wednesday 28, Thursday 29 and Friday 30 - Conference

**** PHYSICS-MATERIALS-BIOLOGY-PATHOLOGY JOINT SYMPOSIA ****

- New microscopies: STM, AFM, SNOM, confocal, ultra-sound, ...
(A. Engel, D. Pohl)

- Energy filtered images and electron spectroscopy
(C. Colliex, D. Bazett-Jones)

- Life in extreme environment (F. Gaill)

**** BIOLOGY-PATHOLOGY SYMPOSIA ****

- Fertilization and early embryogenesis in mammals
(J. E. Flechon)

- Freeze substitution, microscopy of frozen hydrated samples
(J. Dubochet, S. Fakan)

- Image analysis and cancer diagnosis (R. Laurini)

- High-resolution electron microscopy of biological specimens
(E. Delain, M. Muller)

- Cytoskeleton (G. Gabbiani)

- New applications of scanning probe microscopies
(M. Robert-Nicoud)

**** PHYSICS-MATERIALS SYMPOSIA ****

- Electron holography (C. Beeli, D. van Dyck)

- New progress on CBED/LACBED (J. P. Morniroli, J. Steeds)

- High-Resolution microscopy of aperiodic structures
(J. van Landuyt, P.Stadelmann)

- New applications of analytical microscopy: filtered images, EDS, EELS
(C. Colliex, C. Humphreys)

- New applications of scanning probe microscopies (D. Pohl, D. Courjon)

**** Commercial exibition ****

A substantial commercial exhibition of scientific equipment will also be
held during the conference. For information, please contact G. Peter,
EPFL-CIME CH 1015 Lausanne
Fax: +41 [21] 693 44 01, email: peter-at-cime.epfl.ch


********** REGISTRATION **********

The symposia will consist essentially of a small number of lectures (mainly
invited, with some selected from the conference abstracts) and of poster
sessions. The presentations may be given in English, or in any languages
of the three national societies. However, certain parts of the presentation
should be in English, at least abstracts, figure captions, transparencies...
Discussion sessions will be devoted to a few hot points emerging from the
poster presentations.

The registration fees for the conference, including attendance at the
symposia and the abstract booklet, are 250 CHF (non-members), 200 CHF
(members) and 100 CHF (students and lab technicians). The registration fees
for the tutorial days will be communicated in the final circular. A maximum
of 100 CHF/course is expected. A few grants have been founded by the
national societies in order to encourage the participation of young
scientists and technicians


********** GENERAL INFORMATION **********

The conference will be held at the Ecole Polytechnique Federale de Lausanne
(EPFL), located approximately 4 km from the city centre, near (500 m) the
idyllic scenery of lake Leman. Hotel rooms in different categories (from
"Formule 1" to ****) have been reserved by the tourist office for the
organization committee, with prices ranging from 25 (F1, 3 persons per room)
to 230 CHF. A final hotel booking form will be included with the registration
form.

Excursions for participants and accompanying persons are planned: e.g. a
tour to the beautiful region of "la Gruyere" famous for its cheese, to a wine
cellar on the coast of lake Leman !! ... These excursions will be organized
in small groups of 20 to 30 persons according to their preferences.


********** ORGANIZING COMMITTEES **********

Honor committee:
Walter Bollmann, Alain Gautier, Eduard Kellenberger, Bernard Vittoz

International Scientific Committee:
M. Deschuyteneer (SmithKline Beecham, B), J. Dubochet (Uni. Lausanne, CH),
A. Engel (Uni. Basel, CH), J. Gunter (Uni. Zurich, CH),
D. Hernandez-Verdun (Inst. Jacques Monod, F), J. Lecomte-Beckers
(Uni. Liege, B), J.-P. Morniroli (Uni. Lille, F), R. Portier (ENSCP, F),
M. Praet (Uni. Gent, B), D. Schryvers (Uni. Antwerpen, B), P. Stadelmann
(EPFL Lausanne, CH), D. Thomas (Uni. Rennes, F)

Local Scientific Committee:
M. Campiche (Uni. Lausanne), J. Dubochet (Uni. Lausanne),
S. Fakan (Uni. Lausanne), R. Gotthardt (EPFL Lausanne),
R. Laurini (Uni. Lausanne), P. Stadelmann (EPFL Lausanne)

Local Organizing Committee:
P.A. Buffat (Chairman), R. Rouquier (Secretary),
C. Beeli, F. Bobard, B. Garoni, P.-H. Jouneau, D. Laub, G. Peter,
B. Senior, P. Stadelmann


***************************************************************************

For additional information and registration details, please contact
as soon as possible:

Mrs Ruth Rouquier, Congres Trinoculaire,
CIME-EPFL, CH 1015 Lausanne, Switzerland
Fax: (021) 693 4401, Tel: (021) 693 4405
email: ruth.rouquier-at-cime.epfl.ch, http://cimewww.epfl.ch

***************************************************************************



Pierre-Henri Jouneau
Ecole Polytechnique Federale de Lausane
CIME-EPFL, CH-1015 Lausanne
tel: +41 (21) 693 44 37 fax:+41 (21) 693 44 01






From: Giles John E Jr on Thu, Mar 9, 1995 10:07 AM
Date: 9 Mar 1995 10:21:34 U
Subject: Digital Images

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I am looking for information about software to read .bmp or TIFF files using a
Mac. We have a Targa+ video capture board to capture images from the SEM onto
a 486-66 PC. I would like to be able to import them over e-mail to a Mac
Quadra 650 and paste into a Word document. Word will import the image, but
there seem to be a decrease in resolution and the file size is reduced from
650K to 375K when it is converted to the Mac.
I would like to here from other users who are capturing images as we are just
getting the system set up.
Thanks,

John Giles
Honeywell Space Systems
jegiles-at-space.honeywell.com
(813)539-2270 phone
(813)539-3630 Fax





From: ychen-at-macc.wisc.edu
Date: Thu, 9 Mar 1995 12:55:21 -0600
Subject: Re: Polaroid Films

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Message-Id: {25030912503075-at-vms2.macc.wisc.edu}

} I do not know how much extension can be obtained by storage at say
} 4 C (May be someone out there does know). But we seem to start
} having problems with film only a couple of months after the
} expiration date even after cold storage for a few months.
}
} Richard E. Edelmann

I had a problem of inside sticky #52 Polaroid film, which will expire on
Sept. 1995, so that run out of our film. I found a box of old #52 film
which expired on Aug. 1992 left in a drew (at room temperature) and tried
to use it. To my surprise, it worked fine.
For my experiences, the problem of Polaroid does not really related to the
expiration date. Instead, I found a lot of problems are caused by storage.
I have used a lot of good expired films and a lot of fresh films as well
for 5 years.

Ya Chen
Integrated Microscopy Resource
Madison, WI 53706
USA
Email: ychen-at-macc.wisc.edu






From: chandler-at-lamar.ColoState.EDU (John Chandler)
Date: Thu, 9 Mar 1995 08:33:53 -0700
Subject: Re: Fujix Pictrography 3000 Printer

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Message-Id: {v01510101ab84cec55d80-at-[129.82.126.28]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

William Dentler wrote:

} We are in the process of purchasing a new printer and are looking at the
} Tektronix Phaser II SDX, Kodak ColorEase PS, and Kodak 8600. Samples
} provided by
} Kodak and Tektronix look great. I have heard that the Mitsubishi S3600-32U
} is a
} great printer but cannot get any useful information, prices, or samples from
} Mitsubishi or local dealers. I am curious about the Fujix 3000. Could you let
} me know how or where to contact Fujix, since I have never heard of them.

You can contact Ron Saltzman, at Fujix Electronic Imaging Group at
800-736-3600 ext. 8282 (voice mail). If he's no longer with that group,
you might try the general numbers, 914-789-8100 or 800755-3854, to get to a
sales rep. They also advertise in some of the photo lab trade magazines.

Good luck. They are really worth a look.


John chandler-at-lamar.ColoState.EDU Fort Collins CO






From: jerry-at-biochem.dental.upenn.edu
Date: Thu, 9 Mar 1995 10:53:34 -0500
Subject: Carbon Putty

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Thanks to those who have helped me locate sources where I could find
a material matching the description I gave for what I called "carbon putty."

Since posting the first source given to me (Bal-Tec) yesterday, I
have received a few more. So, for those other subscribers who asked me to
post any info. I got, here are the other sources.

SPI Supplies/P.O. Box 656/West Chester, PA 19380/(800)242-4SPI/
Cat.# SPI 5057/$63.75 15g

Marivac LTD./Hailfax, N.S./Canada/(800)565-5821/Cat.# AS508-3/
$66.50 Canadian 15g (Approx. $50.00 American)

SGL-Carbon/Niagara Falls, N.Y./P.O.Box 667/(716)236-2859/
This company makes carbon epoxy material that sets-up for permanent
attachments. It is not a soft putty after it sets but it is highly
conductive and may be of some use for other purposes.

Thanks for all the help --- Jerry





From: jyoung-at-inforamp.net (James Young)
Date: Thu, 9 Mar 1995 18:28:32 +0500
Subject: Info on Agr Sta Closings

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I was talking to Frank Skelton. It seems Agr Cda Van is closing. Any other
closings or layoffs?
Interested only.

JIM Young





From: images1-at-biosci.mbp.missouri.edu (Michael Stanley)
Date: Thu, 9 Mar 1995 18:01:19 -0600
Subject: zamboni fix

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Hello All,
I have a user who was told to try Zamboni Fix on their tissue.
Could some kind soul tell us what it is and how to make it. I know what
and Zamboni is in relation to ice rinks and I can even drive one (it's a
real hoot!!) but I've never heard of the fix...
TIA,

C. Michael Stanley, Ph.D.
Coordinator, Associate Director
Molecular Cytology Core Facility
Molecular Biology Program
2 Tucker Hall
University of Missouri-Columbia
Columbia, MO. 65211
(314) 882-4895
fax= 314-882-0123






From: richard.easingwood-at-stonebow.otago.ac.nz (Richard Easingwood)
Date: Fri, 10 Mar 1995 11:21:44 +1100
Subject: Tissue processors

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Message-Id: {199503092314.MAA28868-at-arwen.otago.ac.nz}
X-Sender: st004718-at-brandywine.otago.ac.nz
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Ed Calomeni wrote:
} I am in the info gathering stage of purschasing a tissue processor for EM
} samples. } I have info on the Reichert-Lynx unit and the one made by RMC. How
} do you like } these units? Comments on advantages and disadvantages are
} welcome.

} Thanks in advance

} Ed Calomeni
} Medical College of Ohio
} Toledo, OH

We have a Lynx tissue processor which we are now very happy with. There
were a few teething problems when it was first installed (the main control
board and the motor control unit had to be replaced) but these problems I
believe have now been sorted out at the factory and shouldn't apply to new
instruments now - it was some three years ago when we bought our one.
The only other problem we had was with voltage spikes from the mains. This
problem was eliminated by putting an isolating transformer between the
mains and the processor.
We have not experienced any problems with the samples mixing upon opening
up the vials (someone -sorry I forget who- mentioned this in an earlier
reply).
Infiltration of resin seems to be fine, something I was a little sceptical
of initially, given the small holes in some of the basket types. There is a
good choice of different specimen baskets anyway and I have always found
one suitable for whatever specimens I happen to be processing.
Hope this is helpful for making your decision.
Regards,
RE



Richard Easingwood
South Campus Electron Microscope Unit
Otago Medical School
PO Box 913
Dunedin
NEW ZEALAND

Telephone: 64-03-479 7301
Facsimile: 64-03-479 7254






From: pmarkiew-at-alchemy.chem.utoronto.ca (Peter Markiewicz)
Date: Wed, 08 Mar 1995 15:50:25 -0500
Subject: AFM deconvolution program

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Message-Id: {199503092054.PAA03408-at-alchemy.chem.utoronto.ca}
X-Sender: pmarkiew-at-alchemy.chem.utoronto.ca (Unverified)
X-Mailer: Windows Eudora Version 1.4.4
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Fellow Microscopists,

This is a general announcement which should be of interest to atomic force
microscopists.

We are making the latest version of our deconvolution program MIDAS95
available for free on the Internet. The program is designed to work under
Windows on Nanoscope files. It is being released as a beta version, meaning
the program lacks a certain polish. The program works properly for both the
Nano II and III, although testing on the latter system has been limited.

Through deconvolution, one can account for the volume occupied by the tip in
the AFM images obtained. By eliminating the tip effect, the result is often
a truer representation of the sample topography. Deconvolution also allows
for the in situ measurement of the tip geometry. This 3D data file of the
tip can be used as a check of the tip's integrity or for improvement of
other AFM images. Further details are given in the README.TXT file.

The program can be accessed by the following:
ftp surfturf.chem.utoronto.ca
login: anonymous
password: yourname-at-location
Please use your E-mail address for the password. We wish to keep a record
of the users of this program and notify them of any problems or updates.

The public directory has three files of interest here. One is the
README.TXT file if you want to know what is in MIDAS.ZIP without having to
download it. MIDAS.ZIP contains several files, including some AFM files to
see how deconvolution is done and some TIFF files for viewing. Fan-xing Wei
and Dr. Dan Thomas of the University of Guelph have modified our program so
that it works for Burleigh Instruments. This program is available in BURL.ZIP.

If you have any difficulty, please be patient as our server allows only one
user at a time and tends to lock up when doing calculations.

Thank you,

Peter Markiewicz





From: waheeschen-at-dow.com (Bill Heeschen (517)636-4005 PMRC/ASL)
Date: Thu, 9 Mar 1995 16:19:57 -0500
Subject: re: Digital Images

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John

The best way to get bitmap and/or TIFF images into your Mac is with the NIH
Image software (via ftp: anonymous-at-zippy.nimh.nih.gov directory /pub/nih-
image). It can handle either format, but you will probably have to go through
the IMPORT diaolog, not the OPEN dialog. Look at the documentation (also on
Zippy) to guide you through. As long as there is a full valid bitmap in the
image file, NIH Image can import the image and allow you to save it as a useful
file. We have done a lot of image file format trouble-shooting for "standard"
formats written by multiple vendors on multiple platforms around here using
that capability.

A plug here: NIH Image has a nearly infinite performance to price ratio - it
is free once you are on the internet. It also outperforms a whole lot of
commercial stuff on any computer platform up to about the $2000 price point and
the support system is unbelievably good, considering the annual maintenance non-
fee. 8-).

Two hurdles are: getting the file onto your Mac (which it sounds like you are
already doing) then getting a good image into Word. I do the latter by pasting
the image from NIH Image into Word, then shift-shrinking the image to about
50%. The original paste goes in at 72 dpi, the shift-shrink gives the final
image resolution of 144 dpi which, with a decent gray-scale printer, gives you
very acceptable print quality. By the way, I __STRONGLY__ advise against using
the image editor in Word. I have found it unreliable in addition to the fact
that it reduces your grayscale image to 16 levels. This could explain the drop
in file size (the editor converts the 8-bit image to 4-bit). A similar result
would happen if you started with a 16-bit TIFF and wound up with 8-bit.

Hope this helps.


Bill
==========================
Bill Heeschen/Analyt. Sci.- Mat. Char. -----
1897-F Building / The Dow Chemical Co. --- ---
Midland, MI 48667 U.S.A. --- D O W ---
phone: (517)636-4005 fax: (517)636-5453 --- ---
Email: waheeschen-at-dow.com -----
========================== R




From: {chswartz-at-MIT.EDU}:ddn:wpafb
Date: 3-8-95 12:54pm
Subject: Re: ultrathinsectioning material containing quartz

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Message-Id: {9503092328.AA21558-at-riker.ml.wpafb.af.mil}
To: WALCKSD:ML:WPAFB
cc: Lloydpf:ML:WPAFB
Subj: ultrathinsectioning material containing quartz
In-Reply-To: Message from WALCKSD:ML:WPAFB of 3-8-95
-----------------------------------------------------------------------
Scott,

I would recommend that the knife of choice be a Diatome knife. The angle
would depend on how hard the sediment is. If the sediment is very hard, then
yes I too suggest a 55x knife. Using a knife with this angle still allows one
to obtain sections on the order of 70nm. If the sediment is not that hard,
then a 45x knife will work also. I generally use a 55x knife for all materials
that I cut except for polymers, where I use a 45x knife.

I hope this helps!

Pam


---------------------- Replied Message Body ----------------------
To: LLOYDPF
Subj: ultrathinsectioning material containing quartz
Forwarded: Message from {chswartz-at-MIT.EDU}:ddn:wpafb of 3-8-95
------------------------------------------------------------------



--------------------- Forwarded Message Body ---------------------
To: WALCKSD:ml:wpafb, SCHELTFJ:ml:wpafb
Subj: ultrathinsectioning material containing quartz
Orig-Author: {chswartz-at-MIT.EDU}:ddn:wpafb
-----------------------------------------------------------
I am going to be attempting to thin section sediment samples containing
quartz and want the thin sections to be approx. 50-70nm in thickness for TEM
analysis. I have to buy a diamond knife and would much appreciate input on the
blade angle to choose. A representative at Polysciences told me I should go to
a 55 degree knife instead of a 45, but I was wondering if I would be
sacrificing
much of the ability to cut sections down to 50nm. The quartz grains are
approx.
200 um in diameter. Is there a particular manufacturer I should get the knife
from? What is an appropriate cutting speed? Can I expect the knife to last
only a VERY short time? I would appreciate any suggestions and advice. My
address is:
chswartz-at-MIT.edu

THAN





From: Mike Gregory :      mgregory-at-pixie.udw.ac.za
Date: Fri, 10 Mar 1995 08:34:29 +0200 (SST)
Subject: Re: BDMA vs DMP-30 summary

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On Mon, 6 Mar 1995, Gib Ahlstrand wrote:


Hi Gib:

I'd appreciate a copy of the BDMA vs DMP-30 responses.

Regards

Mike Gregory

} In July and September of 1994, there was some disciussion of BDMA vs. DMP-30. I
} saved and consolidated 13 of those messages into a single document. If anyone is
} interested in getting a copy, send me your e-mail address and I will zip one out
} to you within a day or two.
}
} --
}
} Gib Ahlstrand, MMS Newsletter Editor
} Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
} 495 Borlaug Hall, St. Paul, MN 55108 (612)625-8249
} 612-625-9728 FAX, giba-at-puccini.crl.umn.edu
}
} "MICROSCOPY & MICROANALYSIS 96" in Minneapolis, Minnesota, Aug.11-15, 1996
}
}




From: Doug Arrell :      ARRELL-at-jrc.nl
Date: Fri, 10 Mar 1995 08:33:09 GMT+0200
Subject: re: Digital Images

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} The best way to get bitmap and/or TIFF images into your Mac is with the NIH
} Image software (via ftp: anonymous-at-zippy.nimh.nih.gov directory /pub/nih-
} image). It can handle either format, but you will probably have to go through
} the IMPORT diaolog, not the OPEN dialog. Look at the documentation (also on
} Zippy) to guide you through. As long as there is a full valid bitmap in the
} image file, NIH Image can import the image and allow you to save it as a useful
} file.

As a follow-up, we use NIH Image for our image processing and also
import BMP and TIFF files from PC capture systems. NIH Image will
import TIFF directly using 'OPEN', and from our experience BMPs
should be IMPORTed using CUSTOM, setting OFFSET to 1760 and chsing
the relevant bitmap size.

I hope this is of use

Doug


+------------------------------------+
| Dr Douglas Arrell |
| Mechanical Performance and Joining |
| Institute for Advanced Materials |
| 1755 ZG Petten |
| Netherlands |
| {ARRELL-at-JRC.NL} |
| Tel. (+31) 2246 5287 |
| Fax (+31) 2246 1917 |
+------------------------------------+




From: epicier-at-univ-lyon1.fr (Thierry Epicier)
Date: Fri, 10 Mar 1995 08:39:58 +0100
Subject: S-SIMPLY update...

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A short message for users of (or people interested in) 'S-SIMPLY'
(SHRLI-SIMulation and diSPLaY of TEM & HRTEM images, for PCs) : the freeware
version, available on FTP.UNIV-LYON1.FR (/pub/dos/HRTEM) has been
significantly updated, and new features are available (graphical displays, a
"Make Interface" tool,...).
With best regards,

______________________________________________

Thierry EPICIER
GEMPPM-502, INSA de Lyon,
69621 VILLEURBANNE, France
tel : (33) 72 43 84 94 (83 85)
FAX : (33) 72 43 85 28
Email : EPICIER-at-CISMSUN.UNIV-LYON1.FR





From: jerry-at-biochem.dental.upenn.edu
Date: Fri, 10 Mar 1995 08:44:59 -0500
Subject: Carbon Putty correction

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Via: bham.ac.uk; Fri, 10 Mar 1995 09:08:17 +0000

To those interested in Carbon Putty,

Two days ago I posted a Cat.# and price for Leit-C-Plast from
Bal-Tec that was given to me incorrectly. The correct Cat.# is B801014077
and the cost is $40.00 for 15g. This is now the best price I've found.

Bal-Tec Products/984 Southford Rd./P.O.Box 1221/Middlebury CT/
(800)875-3713;Fx (203)598-3658

Hope this helps---Jerry





From: Marcel.Paques-at-2488PAS.urlnl.sprint.com
Date: Fri, 10 Mar 1995 10:19:00 -0500
Subject: Fuji Pictography 3000 printer

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I recently purchased a Pictography 3000 printer after a extensive
comparison of printer systems available on the market.
The printer is attached to the network (Token ring, novell) via a pc,
and is transparent configured: all pc's in the network can reach the
printer.
The printer is used for production of hard-copies of image files
generated by various techniques: CSLM, VEM, cryo-SEM, FESEM (+EDS
Noran SUN), TEM (Gatan SSC, Mac), SPM, IA (SEMPER).

We are quit satisfied.

E-mail: Marcel.Paques-at-2488PAS.URLNL.Sprint.Com
Unilever research Laboratory Vlaardingen
The Netherlands




From: A. Kent Christensen :      akc-at-umich.edu
Date: Fri, 10 Mar 1995 10:42:37 -0500 (EST)
Subject: Re: zamboni fix

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Zamboni fix is an EM version of the classical LM Bouin's fix.
Zamboni's original abstract didn't contain any details about how to make
it up. The details are in: Stefanini et al., 1967, Nature 216:173.

A. Kent Christensen
Department of Anatomy and Cell Biology
University of Michigan Medical School
{akc-at-umich.edu}

------------------------------------------

On Thu, 9 Mar 1995, Michael Stanley wrote:

} Hello All,
} I have a user who was told to try Zamboni Fix on their tissue.
} Could some kind soul tell us what it is and how to make it. I know what
} and Zamboni is in relation to ice rinks and I can even drive one (it's a
} real hoot!!) but I've never heard of the fix...
} TIA,
}
} C. Michael Stanley, Ph.D.
} Coordinator, Associate Director
} Molecular Cytology Core Facility
} Molecular Biology Program
} 2 Tucker Hall
} University of Missouri-Columbia
} Columbia, MO. 65211
} (314) 882-4895
} fax= 314-882-0123
}
}
}




From: Smith, Peter :      SMithP-at-agresearch.cri.nz
Date: Thu, 09 Mar 1995 14:14 +1200 (NZST)
Subject: [LM] -Immunocytochemistry method using c

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I have recently been shown a research report in BioTechniques, Vol 13, No
3. (1992) by Reed, J.A., Manahan, L.J., Park, C-S, and Brigati, D.JL
describing an immunocytochemical method based upon capillary action. This
was using the "MicroProbe" marketed by Fisher Scientific, Pittsburgh, PA.

My question is, has anyone used this system? If so what are its advantages
and/or disadvantages . Also could you please give me a contact address, FAX
number or E-Mail for Fisher Scientific.

Thanks in advance.




From: Beverly E Maleeff :      Beverly_E_Maleeff%notes-at-sb.com
Date: 10 Mar 95 10:09:30 ES
Subject: Re: zamboni fix

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Message-Id: {9503101815.AA2900-at-pho018.sb.com}
To: images1 {images1-at-biosci.mbp.missouri.edu} ,
microscopy
{microscopy-at-aaem.amc.anl.gov}

In response to this question:

Hello All,
I have a user who was told to try Zamboni Fix on their tissue.
Could some kind soul tell us what it is and how to make it. I know what
and Zamboni is in relation to ice rinks and I can even drive one (it's a
real hoot!!) but I've never heard of the fix...
TIA,

C. Michael Stanley, Ph.D.
Coordinator, Associate Director
Molecular Cytology Core Facility
Molecular Biology Program
2 Tucker Hall
University of Missouri-Columbia
Columbia, MO. 65211
(314) 882-4895
fax= 314-882-0123


I think that the fixative you're referring to is cited in this paper:
Zamboni, L. and DeMartino, C., 1967. Buffered picric acid-formaldehyde: A new,
rapid fixative
for electron microscopy.

We came across Zamboni's fix when we were perfusion-fixing rat testes a couple
of years ago.
However, it didn't preserve Leydig cell structure as well as some of the other
fixatives we tried.

Hope this info helps. (I want to hear more about driving a Zamboni!)

Bev Maleeff
SmithKline Beecham Pharmaceuticals
Toxicology-US, UE 0462
709 Swedeland Road
King of Prussia, PA 19406
610/270-7987
610/270-7202 fax
e-mail: maleeffbe-at-sb.com





From: Jay Jerome :      jjerome-at-isnet.is.wfu.edu
Date: Fri, 10 Mar 1995 17:12:16 -0500 (EST)
Subject: Meeting Announcement, Atlanta GA

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The 30th annual meeting of the SouthEastern Microscopy Society (SEMS) will be
held on May 17-19, 1995, at the Omni Hotel in Atlanta, GA. Scheduled invited
speakers and their titles are: 1) Nestor Zaluzec, Argonne National
Laboratory, Integrating Computers, Microscopy, and Microanalysis; 2) Phil
Russell, North Carolina State University, Instrumentation and Application of
Scanned Probe Microscopy; 3) Larry Peterson, GBI, Forensic Microscopy; 4)
Terence Mitchell, Los Alamos National Laboratories, Experience with Field
Emission on an SEM, STEM, and TEM; and 5) Jay Jerome, Bowman Gray School of
Medicine of Wake Forest University, Exploring Ultrastructure with
Quantitative 3-D Intermediate Voltage Electron Microscopy. Also on the
program are pre-meeting workshops and tours, contributed papers and posters,
the RUSKA student competition, and commercial exhibits. Social events are the
Wednesday night Exhibitor's Mixer and the Awards Banquet, which will be held
Thursday night at the Fernbank Museum of Natural History

For further
information, contact Janet H. Woodward, SEMS '95 Program Chair, at (912)-945-
3152, FAX (912)-945-3155.



Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-isnet.is.wfu.edu *
**************************************************************





From: jyoung-at-inforamp.net (James Young)
Date: Sat, 11 Mar 1995 20:03:18 +0500
Subject: Your message.

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My goodness I got the message several times. Also the one I sent came back
to me several times. I guess the Government wants to make sure evereything
gets through.

Too bad about the cuts. Frank Skelton told me about Van Closing also I guess
the Farm got hit.

PCI is selling well particularly in the USA and some to Japan.

I have full internet access now. PPP with every application which is
Freeware and Shareware. All windows based and relatively easy to use.

Our site in the office is working under a private account, but we haven't
got the domain name yet. It will be NSCTOR. Anyway I will let you know
when it comes through.





From: Peter Goodhew :      goodhew-at-liverpool.ac.uk
Date: Mon, 13 Mar 1995 08:51:38 GMT
Subject: Re: Electron optics teaching

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David Cockayne asked:
}
} WITH REGARD TO THE QUESTION ABOUT TEACHING OPTICS, CAN ANYONE
ADVISE ME OF WHAT SOFTWARE PACKAGES ARE AVAILABLE FOR TEACHING
ELECTRON OPTICS?

At the same time a similar question was posted on the Microscopy
list (by someone different).

I would be delighted if anyone can update the following list of
teaching software (in the area of microscopy) known to me:

The Institute of Materials (UK) distribute:

The Transmission Electron Microscope by Goodhew
The Scanning Electron Microscope by Humphreys (John)
Electron Diffraction by Goodhew
The Stereographic Projection by Humphreys
Analysis in the Electron Microscope by Goodhew, Humphreys and
Cliff
X-ray Photoelectron Spectroscopy by Watts
X-ray Auger and Laser emission by Goodhew

These are all IBM PC DOS versions, available from Institute of
Materials, 1 Carlton House Terrace, London, SW1Y 5DB, fax
+44(0)171 839 2078
The MATTER project is currently working on greatly-improved
Windows and Mac versions of most of these topics: Completion of
the first two topics (TEM and SEM) is scheduled for the end of
1995. All the above are aimed firmly at teaching, not at research
applications.

There is also an SEM simulation available which originated in
Australia but which is supposed to be downloadable from Nestor
Zaluzec's Microscopy Bulletin Board. However we have consistently
failed to transfer it. Has anyone in the UK succeeded?

I also saw recently a very full simulation of a light microscope.
This is aimed at biologists and was written (and is distributed)
by Maurice Smith at: mol-at-molcol.demon.co.uk

If you can add to this list please reply to both lists [
microscopy and materials-l ]

Thanks

Peter

PS 120+ members of the materials list now!

------------------------------------------------------------------
-----
Professor Peter J Goodhew, Department of Materials Science &
Engineering
University of Liverpool
LIVERPOOL Fax (44) (0)151 794 4675
L69 3BX, UK Tel (44) (0)151 794 4665 (secretary
Debra)
------------------------------------------------------------------
-----
inter alia: Director of the MATTER project for educational
software
------------------------------------------------------------------
-----






From: Fangl-at-fpms.fpms.ac.be
Date: Mon, 13 Mar 1995 12:29:36 +0100
Subject: help

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Dear Sir: Would please give some information about mail list of microscopy.
L.FANG




From: Joyce Craig :      bafpjec-at-uxa.ecn.bgu.edu
Date: Mon, 13 Mar 1995 10:30:19 -0600 (CST)
Subject: Midwest Society of Electron Microscopists

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Midwest Society of Electron Microscopists

STUDENT COMPETITION
TO BE HELD AT
THE UNIVERSITY OF WISCONSIN
WHITEWATER, WISCONSIN
Ambrose Health Center, Room 162
Friday, March 24, 1995
PROGRAM:
1:00 PM: Dr. Ursula K. Charaf, Senior Research Scientist, Johnson Wax:
BLOW IT UP! Applied Industrial Microscopy
1:45 PM: Student Presentations
3:00 PM: Dr. Robert Cardell, University of Cincinnatti Medical Center,
MSA/LAS Sponsored Presidential
Speaker: Modern
Microscopical Approaches to Biomedical Problems
4:00 PM: Reception and Award Presentations

If you are planning to come, telephone or E-mail registrations are
required by March 22.
If you are planning to present your research, please send your abstract
to:
Dr. Lance Urven
University of Wisconsin- Whitewater
800 West Main
Whitewater, WI 53190
urvenl-at-uwwvax.uww.edu
PHONE 414-472-5132
Reception provided by Oxford Instruments Inc.,
Microanalysis Group.:





From: Michael Rock :      merock-at-u.washington.edu
Date: Mon, 13 Mar 1995 10:31:18 -0800 (PST)
Subject: Re: SEM/AFM - CD-recordable, specimen prep.

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Don-
1) the thin film coatings on the polycarbonate are very tightly held
proprietary secrets. try some EDS or WDS.
2) try SEM to determine where these "pits" reside.
3) the "trick" is to plunge the CD into liquid nitrogen (30 sec), then
give it a shot with a hammer. it shears at the interface

we are investigating the CD's by AFM and SEM here in our lab, keep in
touch about further results?

-Mike

On Sun, 12 Mar 1995, Chernoff wrote:

} I am seeking advice regarding specimen preparation. I wish to
} examine the physical pits created when a CD-Recordable disk is
} written. I would like to understand:
} - the physical arrangement of thin film coatings on the
} polycarbonate substrate, i.e. what materials are stacked up, and
} what their typical thicknesses are.
} - what layer contains the physical pits.
} - how to expose that layer for examination by SEM or AFM.
}
} Thanks for your help.
}
} DON CHERNOFF 317-251-1364
} ADVANCED SURFACE MICROSCOPY FAX: 317-254-8690
} 6009 KNYGHTON RD. E-MAIL: echernof-at-ucs.indiana.edu
} INDIANAPOLIS IN 46220 Toll free: 800-374-8557
}
}
}




From: Larry Hawkey :      hawkey-at-neuro.duke.edu
Date: Mon, 13 Mar 1995 14:53:43 -0500
Subject: fair market value for scope?

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Where could I findout what was the fair market value for
a JEOL 1200ex II 5 years old. Basic TEM no Scanning
EDX or stuff like that.
Who has the blue book??

Thank you
larry hawkey
hawkey-at-neuro.duke.edu




From: Davisbl-at-aol.com
Date: Mon, 13 Mar 1995 15:14:53 -0500
Subject: IMAGES

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I'VE BEEN ASKED TO ADDRESS A GROUP OF STUDENTS (LATE HIGH SCHOOL AND EARLY
COLLEGE) A LOCAL COLLEGE SCIENCE CAREER DAY. I AM PLANNING ON USING A
VARIETY OF SEM MICROGRAPHS TO ILLUSTRATE THE APPLICATIONS OF THE SCANNING
ELECTRON MICROSCOPE TO MANY SCIENTIFIC AREAS. IF ANYONE HAS ANY PC-FORMAT
IMAGES THAT THE WOULD BE WILLLING TO E-MAIL TO ME, I WOULD BE VERY GRATEFUL.

THANK YOU ALL IN ADVANCE,
BONNIE DAVIS
E-MAIL:




From: Charles A. Garber
Date: Tue, 14 Mar 1995 02:19:13 EST
Subject: Larry Hawkey; Market Value JEOL 1200EX

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To: Larry Haweky

SPI Supplies

RE: Market Value of JEOL 1200EX

There really isn't anything equivalent to a "blue" book, since there are
just not enough instruments of this type getting sold in order to get any
kind of meaningful sales statistics. However, there are basically three
different types of prices discussed:

a) Selling price of a used instrument from the OEM

b) Selling price from a third party service company

c) Offering price from a former owner


If you want to find out (a), the best person would be Robert Santorelli at
JEOL in Peabody, MA Ph; (800) 343-6766.

For (b), you should contact Mr. Clark Houghton, Secondary Images,
Winchester, OH FAX: (513) 927-5557.

For (c), you should contact again, either Mr. Houghton (above) or Mr. James
Nicolino, X-ray Optics, Florida, Ph: (904) 646-3069 FAX: (904) 565-1897.
Mr. Nicolino services wave length dispersive microprobe systems but has a
good understanding of what different instruments fetch when sold by an
owner, free of any guarantee or warranty, and such sales are generally on
an "as is, where is" basis.

Hope this information will be useful to you.

Charles A. Garber
SPI Supplies
West Chester, PA 19380 USA

Ph: (800) 2424-SPI
FAX: (610) 436-5755
e-mail: GVKM07A-at-prodigy.com





From: GVKM07A-at-prodigy.com (DR CHARLES A GARBER)
Date: Tue, 14 Mar 1995 02:19:01 EST
Subject: RE: ELECTRON OPTICS TEACHING

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--[ FORWARDED PRIVATE MESSAGE ]--------

To: goodhew-at-liverpool.ac.uk Order #4457898
From: GVKM07A
Date: 03/13/95 11:46 PM

Prof. Peter Goodhew

Hello! I think that the soft ware you are thinking of is called the
emTutorial system, and it is indeed produced in Australia. There are
actually two different system programs, emTUTOR and semTUTOR, and they have
been designed to be used as a self-instruction tool, an instruction aid for
class labs and for lecture demonstrations.

Also I think the commercial producers of these really outstanding software
products would be quite unhappy if it should turn out that their
proprietary software was distributed without any benefit to them. I could
be wrong about that, but if I was a betting person, that would be my
opinion.

The product can be ordered from SPI Supplies as SPI #09000-AB and has a
regular price of $345. By coincidence, we have been running a "special"
and until April 15, the price is $250.

There is another new product from the same Australian firm called PARFOCAL,
which is a graphics file conversion program for confocal microscopy and
image analysis. The regular price is $325 but this product is not current
on any kind of special sale.

If you provide a FAX number, we can FAX you additional information.

Charles A. Garber
SPI SUPPLIES (USA)
PO Box 656
West Chester, PA 19380 USA

Ph: (800) 242-4774 Toll free in USA only
(610) 436-5400 Regular phone number
(800) 526-6562 Toll free in Canada only - rings in USA

FAX: (610) 436-5755
1-800-55-7780 Toll free FAX from Ireland only
0800-44-0873 Toll free FAX from New Zealand
0800-89-3066 Toll free FAX from UK



FAX: (610) 436-5755





From: GVKM07A-at-prodigy.com (DR CHARLES A GARBER)
Date: Mon, 13 Mar 1995 22:53:38 EST
Subject: 1st Int'l Conf. EM/Ismalia EGYPT

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I have received the following information from the organizers and wanted to
publicize it to anyone interested in attending or participating:

First International Conference on Electron Microscopy
and
Advances in REsearch in Different Fields of Science

September 2-4, 1995
Ismailia-ETAPT

Sponsored by
Electron Microscopy Center
Suez Canal University
Ismailia EGYPT

Special Topics of the Conference:
1] Role of EM in diagnostic virology
2] Role of EM in diagnosis of tumors cytology and urinary stones
3] Role of EM in ultrastructure pathology of the lung (non neoplastic conditions)
4] X-ray microanalysis: Applications particularly metallurgical,
mineralogical, and biological
5] Scanning EM of plants, animal, insects, and mineral material
6] Study of biological macromolecules from their characteristic electron
diffraction patterns
7] Skin pathology by EM
8] Morphological identification of antigens by EM
9] Different low temperature methods for biological EM
10] Safety measures and maintenance needed for EM

There will be an equipment exhibition in conjunction with this meeting.

Registration for foreigners will be US $150 inclusive of full board during
the time of the meeting.

For further information, contact the organizer:

Prof. Dr. Khalifa Ibrahim Khalifa
Electron Microscope Center
Suez Canal University
Ismailia EGYPT

Ph: (20) 64-226418
(20) 64-333318
FAX: (20) 64-333318 [phone and FAX number]


Message from:

Charles A. Garber
SPI Supplies
PO Box 656
West Chester, PA 19380 USA

Ph: (610) 436-5400
FAX: (610) 436-5755





From: Marcel.Paques-at-2488PAS.urlnl.sprint.com
Date: Tue, 14 Mar 1995 03:41:00 -0500
Subject: mail test m

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X400-Received: by mta merit in /PRMD=internet/ADMD=telemail/C=us/; Relayed; Tue, 14 Mar 1995 03:40:10 -0500
X400-Received: by /ADMD=TELEMAIL/C=US/; Relayed; Tue, 14 Mar 1995 03:38:06 -0500
X400-Received: by /PRMD=SMXFL2/ADMD=TELEMAIL/C=US/; Relayed; Tue, 14 Mar 1995 03:41:49 -0500
X400-Received: by /PRMD=LANGATE/ADMD=TELEMAIL/C=GB/; Relayed; Tue, 14 Mar 1995 03:41:00 -0500

test m




From: Peter Goodhew :      goodhew-at-liverpool.ac.uk
Date: Mon, 13 Mar 1995 08:51:38 GMT
Subject: Re: Electron optics teaching

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--[ FORWARDED PRIVATE MESSAGE ]--------

To: GVKM07A
From: Peter Goodhew
Date: 03/13/95 06:01 AM

David Cockayne asked:
}
} WITH REGARD TO THE QUESTION ABOUT TEACHING OPTICS, CAN ANYONE
ADVISE ME OF WHAT SOFTWARE PACKAGES ARE AVAILABLE FOR TEACHING
ELECTRON OPTICS?

At the same time a similar question was posted on the Microscopy
list (by someone different).

I would be delighted if anyone can update the following list of
teaching software (in the area of microscopy) known to me:

The Institute of Materials (UK) distribute:

The Transmission Electron Microscope by Goodhew
The Scanning Electron Microscope by Humphreys (John)
Electron Diffraction by Goodhew
The Stereographic Projection by Humphreys
Analysis in the Electron Microscope by Goodhew, Humphreys and
Cliff
X-ray Photoelectron Spectroscopy by Watts
X-ray Auger and Laser emission by Goodhew

These are all IBM PC DOS versions, available from Institute of
Materials, 1 Carlton House Terrace, London, SW1Y 5DB, fax
+44(0)171 839 2078
The MATTER project is currently working on greatly-improved
Windows and Mac versions of most of these topics: Completion of
the first two topics (TEM and SEM) is scheduled for the end of
1995. All the above are aimed firmly at teaching, not at research
applications.

There is also an SEM simulation available which originated in
Australia but which is supposed to be downloadable from Nestor
Zaluzec's Microscopy Bulletin Board. However we have consistently
failed to transfer it. Has anyone in the UK succeeded?

I also saw recently a very full simulation of a light microscope.
This is aimed at biologists and was written (and is distributed)
by Maurice Smith at: mol-at-molcol.demon.co.uk

If you can add to this list please reply to both lists [
microscopy and materials-l ]

Thanks

Peter

PS 120+ members of the materials list now!

------------------------------------------------------------ ------
-----
Professor Peter J Goodhew, Department of Materials Science &
Engineering
University of Liverpool
LIVERPOOL Fax (44) (0)151 794 4675
L69 3BX, UK Tel (44) (0)151 794 4665 (secretary
Debra)
------------------------------------------------------------ ------
-----
inter alia: Director of the MATTER project for educational
software
------------------------------------------------------------ ------
-----


-------- Original message header follows --------
From goodhew-at-liverpool.ac.uk Mon Mar 13 06:01:17 1995 [PIM 3.2-342.56]
Received: from AAEM.AMC.ANL.GOV (aaem.amc.anl.gov [146.139.72.3]) by
inetgate.prodigy.com (8.6.10/8.6.9) with SMTP id GAA64180 for
{GVKM07A-at-mail.prodigy.com} ; Mon, 13 Mar 1995 06:01:17 -0500

-------------- End of message ---------------





From: DRStadden:R_D:Armstrong
Date: 3-14-95 8:09am
Subject: AFM Learning Curve

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To: MICROSCO:CENTRAL
cc: DRStadden:R_D:Armstrong
Subj: AFM Learning Curve
------------------------------------------------------------------

One of our researchers is looking into buying an AFM, primarily for
studying latex coatings. As a microscopist (PLM,SEM,EDX) in the
analytical group, I'm interested in the broader applications we might
find for our entire product line.

These are my questions:

1.) What is the "typical" learning curve for the AFM for non-
microscopists vs. microscopists?

2.) How useful is it for one to have access to other types of
microscopies to correlate with AFM results?

3.) Does the usual rule of "fewer operators, less downtime" apply
to any greater or lesser degree with the AFM?

I look forward to any thoughts and experiences you can share.

Dave Stadden
DRStadde+aCENTRAL%ARMSTRONG-at-MCIMAIL.COM
Phone 717-396-5109
FAX 717-396-5865






From: KAKER-at-ctklj.ctk.si
Date: Tue, 14 Mar 1995 14:46:00 +0100 (WET)
Subject: Re:Electron Optics Teaching

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Price list for emTUTOR and semTUTOR (informations from 4th March 1995
direct from Guy Cox Software, Australia):

1. emTUTOR or semTUTOR alone: 100 $
2. Combined package: 160 $
3. Parafocal: 150 S

For detail write or call:

Guy Cox Software
Attn:Guy Cox, M.A., D.Phil.
P.O.Box 366
Rozelle, N.S.W.2039 Australia
Phone & Fax:(02) 818 1896
E-mail:guy-at-emu.su.oz.au

Henrik Kaker
kaker-at-ctklj.ctk.si




From: bjg-at-uniwa.uwa.edu.au (Brendon J. Griffin)
Date: Wed, 15 Mar 1995 00:00:44 +0800
Subject: Australian 'Virtual SEM' teaching package via FTP

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Message-Id: {199503141555.XAA29381-at-uniwa.uwa.edu.au}

Dear All,

I am the author of "Virtual SEM", an earlier version of which called
"SUPERSEM" is available through FTP from the EMMPDL library.

"Virtual SEM 1.2" is an update of this earlier package and is much
enhanced. I'm a bit short of time but the recent discussion (Prof.
Goodhew, and Chuck Garber - regards to both) I think is in part mistaking
my software for that available commercially through SPI and others.

The latest version will be passed to Nestor and John Mansfield in about a
fortnight at the Scanning meeting and I hope they will see fit to update
the old version. It is public domain and comments are most appreciated.
To summarise it is a MAC product utilising real images controlled through a
"SEM console" simulation. It includes tutorial information and two self
assessing tests, the results from which are automatically stored on file.
We have been using it for 18 months and feedback has been good.

The drawback is that it is now around 35 meg in size and I expect it to
reach 40 meg soon. It only needs 4 meg of ram to run on, for example, a
MAC IIsi or better. I developed most of it on a powerbook 180C.

The size is a problem for FTP and as noted in earlier versions I can supply
it on a multisession CD. To do this I am charging US$75 to cover writing
and postal costs only. I will upgrade to later versions and hope to be
able to at postal cost, if I have to hire a bod to do it then would have to
cover the time but cost would still be minimal. We also have a virtual EDS
in development and plan to fully integrate the two.

I will try and answer any queries. Those that have contacted me earlier, I
have not lost your requests, am working my way thru as fast as I can -
please be patient.


Brendon
Brendon J. Griffin
Centre for Microscopy and Microanalysis
The University of Western Australia
Nedlands, WA, AUSTRALIA 6907
ph 61-9-380-2739 fax 61-9-380-1087





From: mullerw-at-rmslab.rockefeller.edu
Date: Tue, 14 Mar 1995 10:59:43 EST
Subject: Microscopy listserver

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Sir or Madam:

I am an Associate Professor at The Rockefeller University interested
in obtaining access to the microscopy listserver. A colleague told me that
this would be a good medium to advertise postdoctoral and technical positions
that may be available in my laboratory.

Please let me know if I need to submit any more information. My address
is:
William A. Muller, MD, PhD
Box 176
The Rockefeller University
1230 York Avenue
New York, NY 10021
Phone (212) 327-8104
Fax (212) 327-8875

e-mail address: mullerw-at-rmslab.rockefeller.edu

Thank you for your help.

Sincerely,


Bill Muller






From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 14 Mar 1995 16:31:43 -0400
Subject: Int'l MAS Conf??

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Message-ID: {n1416923531.369-at-mse.engin.umich.edu}

Subject: Time: 4:23 PM
OFFICE MEMO Int'l MAS Conf?? Date: 3/14/95

I have heard rumors that there will be an international microbeam analysis
meeting in Australia early in 1996. Does anyone know if
this is so; and if it is, do you have any information about when and
where it will be?





From: almonte-at-medcolpa.edu
Date: Tue, 14 Mar 1995 17:04:50 -0400
Subject: FM crossover between FITC & Rhodamine

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I'm staining spinal cord and medulla's neurons from rats' fetuses, 10 days
old, with the following primaries antibodies: Rabbit anti Glycine 1:2500,
1:4000; Rabbit anti GABA 1:100, 1:200; Rabbit anti GAD 1:300, 1:500; and
anti-synaptophysin 1:150, 1:300. I'm using FITC 1:100 or Rhodamine 1:100 as
my secondary antibodies. I keep getting crossover between FITC and
Rhodamine despite the different dilutions of the primary antibodies I have
used. Does anyone has any suggestion?
Thanks in advance,

Ciprian Almonte
Research assistant
Medical College of Pennsylvania
Dept. of Anatomy and Neurobiology
3200 Henry Ave.
Philadelphia, PA 19129
Voice: (215) 842-4081







From: Dave DeFily :      defily-at-tam2000.tamu.edu
Date: Tue, 14 Mar 1995 16:28:18 -0600
Subject: 1.53 vs. 1.57

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We've been trying to measure "integrated density" of some fluorescent images and
found significant differences in the numbers calculated in v. 1.53 and 1.57. We
have 1.53 on a quadra 950 and 1.57 on an 8100. Working with the same image,
1.53 returns numbers approximately 2,000,000 and 1.57 gives us numbers about
400. The mean intensity, Std dev, background, number of pixels...are identical
in both versions. LUTs are also the same in both versions. These are TIFF
images acquired on a DOS machine. I'm not sure if it's related to the
integrated density difference, but in 1.57 we can "open" the TIFF files, while
in 1.53 we have to "import" the TIFF file.

Is there something obvious I'm overlooking? Any suggestions are appreciated.
Thanks

-Dave defily-at-tamu.edu







From: almonte-at-medcolpa.edu
Date: Tue, 14 Mar 1995 17:40:19 -0400
Subject: FITC and Rhodamine crossover????

Contents Retrieved from Microscopy Listserver Archives
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I'm staining spinal cord and medulla's neurons from rats' fetuses, 10 days
old, with the following primaries antibodies: Rabbit anti Glycine 1:2500,
1:4000; Rabbit anti GABA 1:100, 1:200; Rabbit anti GAD 1:300, 1:500; and
anti-synaptophysin 1:150, 1:300. I'm using FITC 1:100 or Rhodamine 1:100 as
my secondary antibodies. I keep getting crossover between FITC and
Rhodamine despite the different dilutions of the primary antibodies I have
used. Does anyone has any suggestion?
Thanks in advance,

Ciprian Almonte
Research assistant
Medical College of Pennsylvania
Dept. of Anatomy and Neurobiology
3200 Henry Ave.
Philadelphia, PA 19129
Voice: (215) 842-4081







From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Tue, 14 Mar 1995 22:45:49 -0600 (CST)
Subject: FTP & WWW at ANL site

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G'day Subscribers....

A general posting since I've had a bunch of questions all
about the same thing.

Yes the EMMPDL/MASLIB/PD Shareware libraries are avaiable
via anonymous FTP. The site is

WWW.AMC.ANL.GOV (= 146.139.72.10 )

Login via standard FTP protocols with
Username=Anonymous
Password=Your Email Address

but please note that you CANNOT access these files using a WWW Browsing program
like Mosiac, Netscape or any other, even though they have FTP
capabilities. You must use a standard FTP protocol client program.
which accesses the conventional FTP ports.

The reason for this is that the although WWW server and the FTP
server are both on the same Computer (WWW.AMC.ANL.GOV=146.139.72.10)
they reside on two different disk partitions which are NOT linked.
The FTP server looks in one place and the WWW server another.
When you login to the WWW site, your browser (client) program
has only access to the disk space assigned to the WWW server hence
it (the WWW program) will NEVER see the FTP files and vice versa!
The reasons for this are a combination of security and convenience
on my part, but with the explosion of the WWW I've created yet
another headache for myself.

So for all of you that have tried and failed this is the most
common reason. If you don't understand this call me next week
sometime and I'll explain, or better yet come to the Telecommunications
Tutorial which I'll be giving at the August MSA meeting.

In retrospect I should have put them on different machines with
different DN's and then the problem would not have happened, but
the damage is done and until I get a chance to come up for air
it will have to stay that way. There is yet another alternative
which is to define an alias on the DNS for the FTP server. If
I can figure out the right person to contact I'll try that route
but be guarenteed it will not be soon.

Just a reminder.....
Abstracts/Papers for the August MSA meeting are due March 15th
i.e. probably the day you receive/read this message.

Cheers...
Your Friendly Neighborhood SysOp

Nestor








From: Greg Begin :      GREGB-at-sales.lmt.com
Date: Wed, 15 Mar 1995 08:11:43 CST6CDT
Subject: ? August Microscopy Meeting

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Howdy All,

If anyone has information on the Microscopy Meeting in August
could you please send information on contacts for the meeting.

Also, I am looking to attend regional meetings and would love to
find a calendar of meetings and places with contacts. I would be
attending for training and exibiting.

Thank You all!!!

P.S. all that are interested about the LaserMaster ImagePrinter
1800 DPI the "NEW" price is $ 6,995.00 and an upgrade from a 60
mhz processor to a 100 mhz. WOW, 1K off plus upgrade.

Greg Begin - LaserMaster Imaging
Specialist in Scientific/Medical Imaging




From: :      MELLIOTT-at-prl.pulmonary.ubc.ca
Date: Wed, 15 Mar 1995 07:23:22 +0800PST
Subject: FITC and Rhodamine crossover????????

Contents Retrieved from Microscopy Listserver Archives
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Just read the posting by Ciprian Almonte concerning crossover between
FITC and Rhodamine and had a couple of questions about what he is
doing. What are your FITC and Rhodamine linked too--goat
anti-rabbit??? Are you trying to do double labelling??? Or is it
that you are getting fluorescence of the FITC at the rhodamine
settings?? More information is required to help.
Yours
Mark Elliott, PhD
UBC-Pulmonary Research Laboratory,
St. Paul's Hospital
Vancouver BC Canada




From: gkennedy-at-ucsd.edu
Date: Wed, 15 Mar 1995 07:42:33 -0800
Subject: FITC/rhodamine

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Message-Id: {199503151536.HAA09760-at-mail.ucsd.edu}
X-Sender: gkennedy-at-popmail.ucsd.edu
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

You need to "fine tune" your microscope filters. Do you perhaps have an older Olympus?? Call your local microscope rep and ask for the correct filters to narrow the band pass of the dichroic filters. Grace






From: :      MELLIOTT-at-prl.pulmonary.ubc.ca
Date: Wed, 15 Mar 1995 07:19:05 +0800PST
Subject: epon alternatives

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I think this may have been discussed by this group earlier but at the
time we were not worried about it so I didn't keep a record of the
responses. One of our tech's asked me to post this:
We are looking for a plastic medium which can replace Epon in
Biological TEM. In our lab we regularly use Epon 812 for its good
stainability of membranes. Recently however, we have just too many
samples to infiltrate and Epon is just too viscous for multiple
tissue transfers. The result is extensive numbers of labour hours.
Please let us know if you have succcessfully worked with any plastic
that has equal stainability of the membranes but is less viscous.
Thanks
Mark Elliott
UBC-Pulmonary Research Laboratory
St. Paul's Hospital
Vancouver BC Canada




From: almonte-at-medcolpa.edu
Date: Wed, 15 Mar 1995 13:42:20 -0400
Subject: Re: FITC and Rhodamine crossover????????

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Just read the posting by Ciprian Almonte concerning crossover between
} FITC and Rhodamine and had a couple of questions about what he is
} doing. What are your FITC and Rhodamine linked too--goat
} anti-rabbit??? Are you trying to do double labelling??? Or is it
} that you are getting fluorescence of the FITC at the rhodamine
} settings?? More information is required to help.
} Yours
} Mark Elliott, PhD
} UBC-Pulmonary Research Laboratory,
} St. Paul's Hospital
} Vancouver BC Canada

FITC and Rhodamine are linked to anti-rabbit, and I'm planning to do double
labelling. My major problem is that I'm getting flourescence of the FITC at
the rhodamine settings and viceversa. Thanks, to all of those that have
replied so far. I appreciate all their suggestions.
Ciprian

Ciprian Almonte
Research assistant
Medical College of Pennsylvania
Dept. of Anatomy and Neurobiology
3200 Henry Ave.
Philadelphia, PA 19129
Voice: (215) 842-4081
E-mail almonte-at-medcolpa.edu







From: Wayne A. Buttermore :      butterw-at-iia.org
Date: Wed, 15 Mar 1995 18:00:25 -0500 (EST)
Subject: Unsubscribe

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Unsubscribe butterw-at-iia.org





From: MacisNo1-at-aol.com
Date: Wed, 15 Mar 1995 21:12:05 -0500
Subject: FITC/ Rhod

Contents Retrieved from Microscopy Listserver Archives
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Try using more selective excitation filters (narrower band pass). Another
suggestion is to use a "Red Cut" Filter. This greatly supresses the secondary
peak that is associated with FITC. Contact you local microscope supplier or
Omega Optical at (802) 254-2690. Good Luck,

Lawrence Kordon
Nikon, Inc.
(410) 740-7404




From: richard.easingwood-at-stonebow.otago.ac.nz (Richard Easingwood)
Date: Thu, 16 Mar 1995 15:50:13 +1100
Subject: SEM of Microcapsules

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Message-Id: {199503160342.QAA24006-at-arwen.otago.ac.nz}
X-Sender: st004718-at-brandywine.otago.ac.nz
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Hello all,
We have a user wanting to look at the inside of Polycaprolactone
Microcapsules in the SEM. We are not wanting to do sections for the TEM.
I have tried to fracture the microcapsules in liquid N2, after embedding
them in resin, but they dont fracture through the capsule, only around it.
Also, I have tried to section the block face of the capsules in resin, but
resin seems to infiltrate into the capsule and so no internal structures
can be seen.
These capsules are between 100 microns - 20 microns in diameter, and have a
melting point around 65oC.
Has anyone tried this before and had success? The references I have
contain no details about preparation of their specimens.
Any help would be appreciated.

Richard Lander.

richard.lander-at-stonebow.otago.ac.nz






From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Wed, 15 Mar 1995 22:54:12 -0600 (CST)
Subject: Int. MAS Meeting (IUMAS)

Contents Retrieved from Microscopy Listserver Archives
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G'day Subscribers (actually in it's now about 11 pm in Chicago)

Here's the info that was posted about the IUMAS meeting that
I dug out of the Archives. You will have to check with Dave
Williams of Lehigh (dbw1-at-lehigh.edu) if you want further details.

Also the WWW site is also starting to get fairly busy, lately
we've been averaging ~ 250 connections/day. So if it
appears slow, consider yourself forwarned.

The FTP server crashed sometime on Monday Evening.
So if anyone tried to get to the libraries, it was
a useless battle. As of a few minutes ago it was back
up and running.


Why does this always happen just before deadlines for
MSA abstracts?

It must be the Ides of March syndrome,
but then again maybe it was a Leprechaun.

G'night all.... Nestor

-------------------------------------------------------------



From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Wed, 15 Mar 1995 22:54:12 -0600 (CST)
Subject: Int. MAS Meeting (IUMAS)

Contents Retrieved from Microscopy Listserver Archives
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MICROBEAM ANALYSIS SOCIETY STUDENT SCHOLARSHIPS TO MAS-1995 (Breckenridge, CO)
and IUMAS-1 (Sydney Australia)

All students (and faculty) involved in microanalysis-related research, should
note a remarkable opportunity for travel to two forthcoming microanalysis
conferences. The Microbeam Analysis society is offering student scholarships to
the 29th Annual MAS meeting in Breckenridge CO (August 6-11, 1995). Student
papers must be submitted, in standard MAS-MSA format to Dr. E. Etz, NIST,
Division 837, Bldg. 222, room Q-113, Gaithersburg, MD 20899 by March 15, 1995.
The best submitted papers will be awarded funds towards attending the annual
meeting in Breckenridge. Any more information about the program can be obtained
from Dr. Etz at etz-at-gapnet.nist.gov

What makes this scholarship offer extraordinary is that the best three papers
given by student scholarship winners at the Breckenridge meeting will be
awarded a minimum of $500 towards the cost of attending the 1st
International Union of
Microbeam Analysis Societies Conference in Sydney, Australia, February 5-9,
1996. These scholarships are only open to student members of MAS, and student
application forms for MAS are available in past issues of Microbeam Analysis,
the MAS journal. Student membership is a great bargain at $2.50, and doesn't
require that the advisor be a MAS member - although if you aren't, I ask that
you consider joining.

I will mail you more information with appropriate details about the meetings
and the paper format etc., but if you have any immediate questions, please don't
hesitate to contact me by email (dbw1-at-lehigh.edu).

Dave Williams






From: Richard E. Edelmann :      EDELMARE-at-CASMAIL.MUOHIO.EDU
Date: Thu, 16 Mar 1995 08:01:30 -500
Subject: Epoxy resin dust saftey

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Can anyone out there point me in the right direction for finding
information on the health risks associated with epoxy resin dust
produced by jewler's saws, jig saws, dremel tools, files, sand paper,
etc.. Yes, I have looked at the various MSDS for the resins but they
haven't provided much real world information (I at least haven't run
into the problem of a tanker truck spillage of NSA).


Richard E. Edelmann
Electron Microscopy Facility Supervisor
352 Biological Science Building
Miami University, Oxford, OH 45056
Ph: 513-529-5712
E-mail: edelmare-at-muohio.edu




From: mrdunlap-at-ucdavis.edu (Michael Dunlap)
Date: Thu, 16 Mar 1995 08:11:26 -0800
Subject: Re: SEM of Microcapsules

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X-Sender: szmdunla-at-bullwinkle.ucdavis.edu
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Richard -

We use double stick tape to fracture the Microcapsules. On a SEM stub we
mount an stip of tape. On tape that is covering the stub we add a small
amount of dried microcapsules. Using an exta piece of tape cover the tape
and capsules and rip off. This will fracture many of the microcapsule so
that you will be able to look inside and outside many of them. Coat the
stubs and view in the SEM.

Good luck

Mike

---------------------------------------------------------------------------
| Michael Dunlap | lab (916) 752-0284 |
| Facility For Advanced Instrumentation | fax (510) 422-2282 |
| University of California | mrdunlap-at-ucdavis.edu |
| Davis CA, 95616 | |
===========================================================================






From: Louisa.Howard-at-Dartmouth.EDU (Louisa Howard)
Date: 16 Mar 95 11:16:20 EST
Subject: TEM and LM on cell monolayer

Contents Retrieved from Microscopy Listserver Archives
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Message-id: {14465545-at-blitzen.Dartmouth.EDU}

I have done many TEM preparations on cell monolayers, using tissue culture
dishes and/or glass coverslips. There is a lab here that would like to do light
microscopy and TEM on a single cell in a monolayer. I assume this can be done,
if I had a coverslip that was etched with a marker grid. This marked grid
pattern would have to show on the epoxy face, after the coverslip was separated
from the embedding media. Can anyone tell me if there are glass or plastic
coverslips that have some type of etched grid pattern that could be used in
this way and where they could be ordered in the U.S.

I would prefer working with a plastic/tissue culture type of coverslip, as
they are easier to remove before sectioning.

thanks
Louisa Howard
Ripple Electron Micrscope Facility
Dartmouth College
Hanover, N.H 03755
Louisa.Howard-at-Dartmouth.edu




From: Louisa.Howard-at-Dartmouth.EDU (Louisa Howard)
Date: 16 Mar 95 11:36:20 EST
Subject: TEM and LM of cell monolayer

Contents Retrieved from Microscopy Listserver Archives
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Message-id: {14465851-at-blitzen.Dartmouth.EDU}

I have done many TEM preparations on cell monolayers, using tissue culture
dishes and/or glass coverslips. There is a lab here that would like to do light
microscopy and TEM on a single cell in a monolayer. I assume this can be done,
if I had a coverslip that was etched with a marker grid. This marked grid
pattern would have to show on the epoxy face, after the coverslip was separated
from the embedding media. Can anyone tell me if there are glass or plastic
coverslips that have some type of etched grid pattern that could be used in
this way and where they could be ordered in the U.S.

I would prefer working with a plastic/tissue culture type of coverslip, as
they are easier to remove before sectioning.

thanks
Louisa Howard
Ripple Electron Micrscope Facility
Dartmouth College
Hanover, N.H 03755
Louisa.Howard-at-Dartmouth.edu




From: Louisa.Howard-at-Dartmouth.EDU (Louisa Howard)
Date: 16 Mar 95 12:56:52 EST
Subject: TEM and LM of cell monolayer

Contents Retrieved from Microscopy Listserver Archives
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Message-id: {14466868-at-blitzen.Dartmouth.EDU}

I have done many TEM preparations on cell monolayers, using tissue culture
dishes and/or glass coverslips. There is a lab here that would like to do light
microscopy and TEM on a single cell in a monolayer. I assume this can be done,
if I had a coverslip that was etched with a marker grid. This marked grid
pattern would have to show on the epoxy face, after the coverslip was separated
from the embedding media. Can anyone tell me if there are glass or plastic
coverslips that have some type of etched grid pattern that could be used in
this way and where they could be ordered in the U.S.

I would prefer working with a plastic/tissue culture type of coverslip, as
they are easier to remove before sectioning.

Thanks.

Louisa Howard
Ripple Electron Micrscope Facility
Dartmouth College
Hanover, N.H 03755
Louisa.Howard-at-Dartmouth.edu




From: nina allen :      allen-at-wfu.edu
Date: Thu, 16 Mar 1995 14:54:55 -0500 (EST)
Subject: Re: TEM and LM of cell monolayer

Contents Retrieved from Microscopy Listserver Archives
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If you are going to look at cells in a light microscope, it is a poor
idea to use plastic coverslips. You will not get a good image in
Differential Interference Contrast or Polarized light...as the cover slip
may be strained, and so forth. N.Allen




From: sfzane-at-unccvm.uncc.edu (Sandra F. Zane)
Date: Thu, 16 Mar 1995 15:32:58 -0500
Subject: curing resins

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Hello Microscopists,
Although I can't help Richard Edelmann with his question regarding
resin dust except to say that we do use dust masks when sawing or cutting
blocks; I would like to pose a question closely related to this.
How many of you have your curing ovens vented to a hood?
We have a VERY low volumn of resins being cured at any given time and
have sought, somewhat unsuccessfully, information that would help us
determine at what level it would be necessary to do this. As Richard said,
the MSDS sheets don't provide much real world information. I did call one
of the EM suppliers (I don't recall which one now), and was told that
venting to a hood would be necessary only in an industrial situation.
I would be very interested in hearing your opinions and how you handle
this problem.
I would also be willing to summarize any responses and post that to the
list.

Thank you very much, Sandra Zane

Sandra F. Zane, EM Tech.
Biol. Dept. UNCC
Charlotte, NC 28223
sfzane-at-unccvm.uncc.edu
Fax (704) 547-3128





From: Patty Jansma :      plj-at-manduca.neurobio.arizona.edu
Date: Thu, 16 Mar 1995 13:52:43 -0700 (MST)
Subject: Re: TEM and LM of cell monolayer

Contents Retrieved from Microscopy Listserver Archives
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We use glass gridded coverslips for tracking cells. You can get them from
Bellco Glass, Inc. P.O. Box 340 Edrudo Road Vineland, New Jersey 08360
Cat. # 1916 -92525 Etched gridded coverslips 1 case approx. $30.00.


Patty Jansma Tel:602-621-6671
plj-at-manduca.neurobio.arizona.edu
Arizona Research Labs Division of Neurobiology
University of Arizona


On 16 Mar 1995, Louisa Howard wrote:

} I have done many TEM preparations on cell monolayers, using tissue culture
} dishes and/or glass coverslips. There is a lab here that would like to do light
} microscopy and TEM on a single cell in a monolayer. I assume this can be done,
} if I had a coverslip that was etched with a marker grid. This marked grid
} pattern would have to show on the epoxy face, after the coverslip was separated
} from the embedding media. Can anyone tell me if there are glass or plastic
} coverslips that have some type of etched grid pattern that could be used in
} this way and where they could be ordered in the U.S.
}
} I would prefer working with a plastic/tissue culture type of coverslip, as
} they are easier to remove before sectioning.
}
} Thanks.
}
} Louisa Howard
} Ripple Electron Micrscope Facility
} Dartmouth College
} Hanover, N.H 03755
} Louisa.Howard-at-Dartmouth.edu







From: sfzane-at-unccvm.uncc.edu (Sandra F. Zane)
Date: Thu, 16 Mar 1995 16:08:03 -0500
Subject: EM:curing resins

Contents Retrieved from Microscopy Listserver Archives
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Hello Microscopists,
Although I can't help Richard Edelmann with his question regarding
resin dust except to say that we do use dust masks when sawing or cutting
blocks; I would like to pose a question closely related to this.
How many of you have your curing ovens vented to a hood?
We have a VERY low volumn of resins being cured at any given time and
have sought, somewhat unsuccessfully, information that would help us
determine at what level it would be necessary to do this. As Richard said,
the MSDS sheets don't provide much real world information. I did call one
of the EM suppliers (I don't recall which one now), and was told that
venting to a hood would be necessary only in an industrial situation.
I would be very interested in hearing your opinions and how you handle
this problem.
I would also be willing to summarize any responses and post that to the
list.

Thank you very much, Sandra Zane

Sandra F. Zane, EM Tech.
Biol. Dept. UNCC
Charlotte, NC 28223
sfzane-at-unccvm.uncc.edu
Fax (704) 547-3128





From: jthompso-at-wiley.csusb.edu (Jeff Thompson)
Date: Thu, 16 Mar 1995 14:00:42 -0800
Subject: Re: TEM and LM of cell monolayer

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Glass coverslips etched with an alphanumeric grid are available
from Bellco Glass, Inc. (800-257-7043). I have successfully grown cells in
culture on these coverslips for LM and SEM analysis after coating the
coverslips with either poly-lysine or collagen. Identified cells are
relatively easy to re-identify when going from one system to the other. I
have not embedded cells grown on these coverslips, so I do not know if the
grid will show up on the surface of the plastic when the coverslip is
removed.

Jeff Thompson, Ph.D.
Director, Electron Microscope and Image Analysis Center
California State University
San Bernardino, CA 92407
jthompso-at-wiley.csusb.edu





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Thu, 16 Mar 1995 23:50:47 -0600 (CST)
Subject: Nanotubes & X-sections

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Message-Id: {199503170423.AA27446-at-pccms.pku.edu.cn}


I must confess I'm a bit perplexed by Z.Q. Liu's question about
x-sections of Nano-tubes. If they are truely "nanometer" in size
why would you attempt to x-section them? Are they actually larger?
All the EM I've seen about nanotubes did not involve x-section, but
simple direct imaging.

Okay so let me ask the dumb question. How big are they really?

Nestor....
Your Friendly Neighborhood SysOp.




From: Leszek Kepinski :      kepinski-at-highscreen.int.pan.wroc.pl
Date: Fri, 17 Mar 1995 12:09:15 +0100 (CET)
Subject: HRTEM negatives - digitalization

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What is the appropriate way to digitize TEM negatives for HRTEM work at
resolution of approx.0.23 nm? Please give approximate cost of the digitizing
hardware when possible.
Leszek Kepinski


*****************************************************************
Leszek Kepinski
Institute of Low Temperature and Structure Research,
Polish Academy of Sciences, P.O.Box 937, 50-950 Wroclaw, Poland
tel:(4871) 350 21 ext.153
fax:(4871) 44 10 29
email: kepinski-at-highscreen.int.pan.wroc.pl
*****************************************************************




From: Carl Henderson :      chender-at-umich.edu
Date: Fri, 17 Mar 1995 09:48:00 -0500 (EST)
Subject: Re: Mprobe.Geol

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On Fri, 17 Mar 1995, Robert McDonald wrote:

} I have been asked to analyse some micas for Rb and from a
} preliminary examination it looks like I should use the L alpha
} line - except that the Si K alpha is rather close making the
} Rb sit on the slope to the right of it.
}
} Anyone have any advice on backgrounds etc?
}
} I am using TAP xtals, 25 kV,55 nA with a counting time of
} 60 seconds for peak and background - is this reasonable?
}
} Machine is Cameca SX50.

25kV, 55 nA sounds pretty high for routine mica analyses, though it might
be OK to analyse the Rb at this condition after analysing the major
elements. At 25kV, you could use the Rb KA line on an LIF crystal and
avoid the Si overlap. The counting times sound reasonable, though you
may have to increase them if you are really interested in "trace"
analysis of low levels of Rb.

I suspect that you will always obtain "false" Rb when analysing Rb LA on
TAP in the presence of high Si in your sample. The Si KA peak is about
-750 steps from the Rb LA line (and about +200 from the Rb LB line). This
is far enough that most of the Si peak will be resolved, but there will
probably be some tail effect of the Si under the Rb LA peak. You can
check for this be analysing SiO2. This will give you the worst case Si
interference for silicates. You could continue this process and draw up a
working line (curve) of wt.% Si vs. wt.% measured Rb by measuring various
silicates with differing Si contents and no Rb. You could then use this
curve to correct for your analyses of Rb on your unknowns.

The SX50 software should also give you the chance to use a background
"slope" instead of having to take background counts at both +bkgd and
-bkgd positions. The "slope" on the older CAMECA MBX software was defined
as (bkdg+ + bkgd-)/(2*bkgd+). Thus, a "flat" background would have a
slope of 1.000, while a background that increases with lower sin(theta)
will have a slope } 1.000. Using a background slope will not solve the
tail effect of the Si under the Rb LA, but may help you in using the Rb KA
line on LIF, where the bkgd- position may be out of range for the
spectrometer.

Good luck!

Carl

Carl Henderson
Electron Microbeam Analysis Laboratory
University of Michigan
2005 C.C. Little Bldg.
Ann Arbor, MI 48109-1063 USA
-----------------------------------------------------
(313) 936-1550 (voice) **** Next time, ****
(313) 763-4690 (FAX) *** take the ***
chender-at-umich.edu (e-mail) **** train! ****
-----------------------------------------------------




From: tivol-at-tethys.ph.albany.edu
Date: Fri, 17 Mar 1995 10:33:50 EST
Subject: Re: Nanotubes & X-sections

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Dear Nestor,
John Cowley gave me reprints of some of his recent papers in which
there are images of nanotubes which are on the order of 10 nm across (O.D.).
He and his co-workers have found different structures at various places in
the nanotubes, so I can see why someone might wish to section them (although
it is hard to imagine how this can be done without perturbing (destroying?)
the structures). John would likely send you reprints if you asked him.
Yours,
Bill Tivol




From: tivol-at-tethys.ph.albany.edu
Date: Fri, 17 Mar 1995 10:55:30 EST
Subject: Re: HRTEM negatives - digitization

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Dear Leszek,
In order to retain the original resolution (0.23 nm), the digitization
should have a pixel size corresponding to no more than half that resolution.
That is, if the magnification of the negative is 100,000x, the pixel size must
be ~10 micrometers. This can be done on (among other brands) a Perkin-Elmer
flatbed microdensitometer (cost ~ $100,000). A much cheaper alternative is
a CCD camera. By arranging to image the negative with the CCD so that the
pixels are ~10 micrometers you can get the required resolution. This means
that a 1000*1000 CCD array will look at a 1 cm**2 area. This can be done at
a cost of ~$50,000 (maybe less--I'm no expert here). You also save scan time
with the CCD; however, quantitation of intensities--especially for ED patterns
etc. where there are regions of very high OD surrounded be regions of low OD--
is much worse with CCD than with a flatbed scanner. Good luck.
Yours,
Bill Tivol




From: Larry Allard :      allardlfjr-at-ma160.ms.ornl.gov
Date: 17 Mar 1995 08:42:26 U
Subject: nanotube x-sect.

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Message-ID: {n1416690645.11463-at-ma160.ms.ornl.gov}

Subject: Time: 8:16 AM
OFFICE MEMO nanotube x-sect. Date: 3/17/95

Can standard TEM images normal to the axis of the nanotube determine
unambiguously the cross sectional shape of tubes (i.e. whether the tubes are
facetted, circular cylinders, oval-shaped, hollow or filled with amorphous
material, etc?). We think there are 2 ways to get this information: direct
imaging of cross-sections or electron holography (in which case you don't
need cross-sections). The problem with cross-sections is the potential
development of artifacts during the preparation process. We suggest strongly
to use electron holography (see for example: J. Mater. Sci., 29 (1994)
5612-5614 (this is on Pd particles, but the principle is the same). Also see
our paper which includes nanotubes in the Proceedings of the 1st Int'l
Workshop on Electron Holography, Elsevier, in press.





From: ldm-at-apollo.numis.nwu.edu (L. D. Marks)
Date: Fri, 17 Mar 1995 11:56:54 -0600
Subject: Re: HRTEM negatives - digitization

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Bill Tivol said in his email that "the digitization should have a pixel size corresponding to no more than half that resolution". I think that this is a
typo: you need at least 2 sampling points for a given wave. Therefore if
one wants to quantify 0.23nm spacings in HREM the smallest sampling is half
this (Nyquist limit) and } 6 samplings is more standard.




From: Larry Allard :      allardlfjr-at-ma160.ms.ornl.gov
Date: 17 Mar 1995 15:35:47 U
Subject: Digitizing TEM negatives

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I've repeated the original question below, in case someone's missed the
thread. I suspect that Liu is trying to make cross-sections to
characterize growth mechanisms. Nanotubes, like asbestos fibers, are
easily examined in plan-view, since their high aspect ratio virtually
guarantees that they will lie flat on a film with the long axis parallel
to the film surface. Most of the pictures that I have seen claiming to
be nanutubes in cross-section had no supporting evidence that they were
anything but an essentially spherically symmetric "bucky onion."

In order to get a true nanotube cross section, one will first probably
have to anneal the mass to remove all lesser fullerenes, including the
onions. Then break the mass up and embed in a hard epoxy. Although
ultramicrotomy isn't a bad idea, there is no real way to align the
nanotubes and I'm not certain what effect this would have on cutting.
It is probably better just to sandwich between silicon and do a standard
cross-section by mechanical polishing, dimpling, and ion-milling. It is
going to be hard to prevent rapid milling of the epoxy as opposed to the
tubes, so it is important to mechanically thin as far as possible before
ion-milling (hence, the silicon as an optical guide).

That's my 2 cents worth.

Daniel L. Callahan
Department of Mechanical Engg. and Materials Science
Rice University
dlc-at-owlnet.rice.edu
---------- Forwarded message ----------

Subject: Time: 3:27 PM
OFFICE MEMO Digitizing TEM negatives Date: 3/17/95

Regarding the recent debate on negative scanning:
The Ektron scanner is {$30K, and gives a highly linear image up to 4096 x
4096 pixels. It will permit accurate density corrections to be done for best
quantitative results (see Voelkl, et al., Ultramicroscopy 55 (1994) 75-89,
for all you could ever want to know about scanning negatives and density
corrections etc.)





From: tivol-at-tethys.ph.albany.edu
Date: Fri, 17 Mar 1995 16:22:54 EST
Subject: Re: Re: HRTEM negs - digit

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L.D. Marks is correct--I meant a pixel size corresponding to twice the reso-
lution, or half the size. I always seem to have trouble writing about things
for which less is better :-).
Yours,
Bill Tivol




From: MILLERS-at-NCCCOT.AGR.CA
Date: 17 Mar 1995 10:17:34 -0500 (EST)
Subject: microscopical society of canada annual meeting

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Microscopical Society of Canada
22nd Annual Meeting

The MSC Executive and the Local Organising Committee cordially invite you to attend
and participate in the 22nd Annual Meeting of the Microscopical Society of Canada.
This meeting will be held in the University Centre Building, University of Ottawa, Ottawa,
Ontario, Canada, June 4-7, 1995. A varied and interesting scientific program has been
planned and will consist of a combination of interdisciplinary symposia presented by
speakers from around the world, separate physical and biological symposia, oral and
poster presentations, workshops on TEM specimen preparation of materials and cryo-
SEM specimen preparation and X-ray analysis, and commercial exhibits.

Local Organising Committee

Jim Corbett (Chairman, Secretary, University of Ottawa Liaison)
John McCaffrey (Treasurer, Space Management, Social Program, Accommodation)
Jeff Fraser (Commercial Exhibits, Social Program, Accommodation)
Graham Carpenter (Scientific Program Chair, Materials)
Larry Arsenault (Scientific Program Chair, Biology)
Peter Sewell (Corporate Liaison, Commercial Exhibits)
Kamal Botros (Scientific Program)
Louise Weaver (Scientific Program)
Paula Allan-Wojtas (Registration)
Shea Miller (Registration)

DEADLINE FOR RECEIPT OF ABSTRACTS: March 31, 1995

DEADLINE FOR PRE-REGISTRATION: May 1, 1995

For further information contact:

Program: Registration:

Jim Corbett Shea Miller or Paula Allan-Wojtas
Department of Physics Centre for Food and Animal Research
University of Waterloo Agriculture and Agri-Food Canada
Waterloo, Ontario Room 2016, K.W. Neatby Bldg.
Canada, N2L 3G1 Central Experimental Farm
Ottawa, Ontario, Canada, K1A 0C6
Tel: (519) 885-1211 Tel: (613) 957-4347, ext. 7908 (Shea),
7970 (Paula)
Fax: (519) 746-8115 Fax: (613) 943-2353
e-mail: corbett-at-physics.watstar.uwaterloo.ca e-mail: millers-at-ncccot.agr.ca
allanwojtasp-at-ncccot.agr.ca


PRELIMINARY MEETING PROGRAM

INTERDISCIPLINARY SYMPOSIUM
ADVANCES IN MICROSCOPY


J. Watson (Michigan State University) Events in science and microscopy
J. Hillier (Princeton University) Early developments in EM
A. Eades (University of Illinois) Electron microscopes of the future
O. Krivanek (Gatan Inc) Nanoscale elemental and chemical
mapping
M. Hansen (FEI Inc.) A comparison of LaB6 and CeB6
thermionic cathodes
I.A. Rauf (Queen's University) STM, AFM, CTEM and STEM imaging
of polycrystalline tin-doped indium
oxide thin films
H.J. Kreuzer (Dalhousie University) Lensless low-voltage electron
holography

MATERIALS SCIENCES

D. Dorset (University of Buffalo) Direct structure determination by
electron crystallography
A. Eades (University of Illinois) RHEED: progress towards the
extraction of quantitative information
P. Midgley (University of Bristol) Electron diffraction for structure
determination
D. McCombe (McMaster University) Scanning tunnelling microscopy of
semiconductor surfaces and
interfaces
D. Perovic (University of Toronto) Direct imaging of semiconductor
dopants by EM
M. Loretto (University of Birmingham) Analytical TEM of advanced
materials
G. Weatherly (McMaster University) Microstructures of SiC-reinforced Mg
casting alloys
C. Hansen (Queen's University) The application of environmental
SEM to studies of concrete

BIOLOGICAL SCIENCES

W. Chiu (Baylor College of Medicine) Cryo-imaging
P. Ottensmeyer (University of Toronto) Imaging and computer analysis
H. Hagler (University of Texas) Calcium imaging
P. Ingram (Research Triangle Inst) Elemental mapping
P. Echlin (Cambridge University) Cryo-methods


WORKSHOPS



TEM specimen preparation for Materials Science
Topics include: Ion-beam sputtering
Cleaving
Ultramicrotomy
Cross sections, etc.



Cryo-SEM specimen preparation and X-ray analysis
Topics include: Fracturing and cryo-planing for morphometric and elemental
analysis
Cryo-conductive coating
Standards for frozen hydrated specimens
Light element detection and quantification



Satellite Workshop (June 8, Central Experimental Farm, Ottawa)
Applications of Microscopy in Agricultural Research
Topics include: Preparative techniques
3-D view of the cell
Microtubules
Soils in agricultural use
Electron microscopy in dairy research
Entomology
Image analysis-seed quality
Image analysis of SDS-PAGE gels
Spore development
Immunogold labelling
Food microstructure




From: DVCCO-at-aol.com
Date: Sun, 19 Mar 1995 03:54:20 -0500
Subject: Video Camera Selection Guide2of3

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DVC Video Camera Guide And Release 2 of 2

Remember, if the camera is on the ragged edge of 8 bit performance and
you want to add gain for more sensitivity, even the smallest amount of
gain might put you in the 7 bit range if you we not there already.
If you have 10 bit capability on your board why not use a 10 bit camera.
* Also a camera specification of 60dB is not a 10 bit camera only an 8.
* Also there are many types of noise in a sensor besides thermal noise.
Cooling a sensor will reduce (thermal noise only) and allow you to
integrate longer. I would double check the impression some make that
when a 50db sensor is cooled it offers a better s/n of 10 or 12 bits vs
just 7.
The reduction of one type of noise (thermal) is all that cooling
provides.
* Other types of sensor noise besides dark current include photon, nyquest,
shot, gain noise, and flicker noise etc.
* The signal to noise of the sensor at room temperature is a good measure
of what you can expect.
** Look at the quantum efficiency at the nm range needed.
** look at the spectral response of the sensor.
** There are different grades of sensors available relative to dead
pixels and gray level variation on other manufacturers cameras.
*Digital RS-422 eliminates pixel jitter for sub-pixel accuracy if it is
needs versus the genlock action between the digitizer board and the
camera as they try to lock to each other in the RS-170 analog mode.
*Digital cameras of the higher end variety typically offer 7.5 frames/sec
vs 30 (real time) or even less depending on the amount of pixels you need
to process. If you wish to do real time imaging 30fps, 7.5fps will not
do. Also for fluorescence the shortest duration exposure is preferred.
* The camera has to have a good performance to cost ratio. At the low end
there are plenty of security type cameras out there, but will they offer
you the performance you require.
* The big point here is that the higher the signal to noise, the quieter
the signal, the more gain that can be added to the signal/ which adds noise
as a result, but allows the user to get more sensitivity.
Thus if you start with the quietest / highest signal to noise obtainable
the better off you are when trying to get more gray levels with minimum
sensitivity or more signal to noise allows more gain to be added to achieve
a set sensitivity far superior to a signal to noise start point less than
the DVC.

The following features listed below are offered by the DVC camera line:

General Statement: Analog video RS-170 focus
*Most digitizer boards are typically 8 bits some go higher with digital
RS-422
input. With Dc's 10 bits of signal on the analog RS-170 video, this offers
first of all more top end room for adding more sensitivity and still
maintaining
8 bits/ 256 gray levels for the digitizer board.
*You will see more digitizer boards with 10 bit capability on the analog
RS-170
input. What will you use as a real time 30 frame / sec camera to match that
feature. With most ( real time ) cameras being in the 7 bit range, they
would
better qualify for security applications. There are some 8 bit cameras, but
they only offer 256 gray levels with not much room to add gain for more
sensitivity. The DVC camera line offers 10 bits or 1024 gray levels output
for those boards along with simultaneous digital RS-422 on the digital models
One of the digitizer boards with a 10 bit analog front end is Mutech. They
are the only one presently that has that capability for now and others will
follow soon.

* Very high signal to noise } 62dB at .5 lux at 30fps/ real time
* Frame transfer 1/2" format sensor with 755 horizontal by 484 vertical
pixels, or 565 TV lines horizontal by 350 TV lines vertical resolution.
* The only upgradable research camera with the researchers funding
limitations in mind using the same high quality sensor on all 3 models.
Upgradability from the RS-170 analog unit to the dual output 8 bit
digital or to the dual output 10 bit digital models with RS-170 analog.
(This allows the camera to grow with the researcher) and his budget.
See part 3 of 3 next.




From: DVCCO-at-aol.com
Date: Sun, 19 Mar 1995 03:52:28 -0500
Subject: Guide to Choosing A CCD Video Camera With New DVC Product Release

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And Digitizer Selection Guide For Benefit of Microscope Users Group

Monochrome Digital And Analog Video Camera Line By DVC Company
Part 1 of 3 due to capacity limitations of e-mail
*****************************************************************
With all due respect: If anyone is offended by a guide to choosing a
CCD video camera with a new product release that is
applicable for the users in this group ( HIT YOUR DELETE KEY NOW! )
There are some users that might appreciate this information.
(This was done with good intentions) so any providers of negative feedback
have been given the DELETE KEY OPTION and need not waste net time, thank you.
Valid questions are appreciated and I will try to answer them relative to
the volume I receive. Included is list of RS-422 digitizer boards in part 2
and 3.
* Please address any inquiry directly to dvcco-at-aol.com, not the main file
server.
*****************************************************************
Dear Microscope Users,

I wish to share some data with you on analog and digital monochrome cameras
that I thought you might be able to make use of when reviewing your present
or future camera situation. Some of the below information you might be aware
of, but others might not. If anyone belongs to other pertinent groups and
believes this information to be of benefit to that particular group we would
appreciate you letting us know of them.

Monochrome cameras offer much superior gray level and resolution performance
along with higher signal to noise specs than the typical color versions.
Everyone has different needs and the data below is for researchers who have
decided to utilize the benefits of monochrome cameras. Adding pseudo colors
to the many more gray levels attainable with a monochrome camera might be a
option for you, or adding a tunable liquid crystal filter in line with the
camera for serial Red, Green, Blue could also be a option if accurate
correlation of the pixel is important. For monochrome applications a
electronic tunable filter exists that is very selective with 5,7,10,15,20
,35 or 50nm band pass filters from 450 to 1050 nm range tunable
electronically!
See 2 of 2 for info on tunable filters.

Some things to look for in a research grade monochrome camera are the
following:
* The highest signal to noise possible in a real time 30 frames/sec. unit.
Signal to noise is the ultimate measure of a camera and no amount of
manipulation, bells and whistles, or 20 different knobs to adjust, will
change the bottom line which is the amount of gray levels obtainable.
* No geometric distortion- CCD cameras offer basically no distortion while
any tube camera gives you distortion in the } 5% range. My opinion is
that to use a tube camera where a ccd unit could be substituted is just
qualitative, not quantitative microscopy.
* No dead pixels on your sensor and lowest pixel variation with no false
fill-ins of pixels with data from the pixel next to it by use of memory
circuits due to imperfections in the manufacturing process.
Different types of CCD cameras have their uses.
The 2 main types are frame transfer (FT) and interline transfer (IT)
types for microscopy. CID types are also out there but have uses more in
machine vision applications.
Interline transfer sensors have large gaps between the pixels due to the
construction. They offer 100% fill factor by the use of plastic
micro lenses that help their sensitivity due to smaller well capacity,
but still have the ( same smaller wells.) Plastic does not offer good
performance down in the UV range below 400nm, and have other limitations
at the near IR range. 1/3 format sensors have even less well capacity
and I feel are only produced to provide better yields/profit for the
manufacturers for security applications.

Frame transfer sensors which DVC uses have dynamic range of 70dB to start
and have no dead pixels and no false fill-ins. The wells are very
large and the dark current is {30 electrons at 25C, room temperature.
The pixels are contiguous, or within angstroms of electrical separation.
Larger wells offer higher signal to noise. Double correlated sample and
hold circuits built right into the sensor help reduce noise further on
some frame transfer sensors. Frame transfer units offer one half the
vertical
resolution when doing integration and shuttering due the field transfer
technique used.
Spectral range with glass face plate attached 400-1100nm. Customers
doing (laser) work have reported response in the low 200nm range with
face plate removed and up to 1300nm.

Typical bit vs signal to noise ranges:
7 bits = 44dB to technically 50dB but more like 54dB realistically
8 bits = 55dB to 61dB or 256 gray levels
10 bits = 62dB+ for 1024 gray levels. **10 bits offers 4 times the
performance of an 8 bit camera.
* Another words a camera specification of 50dB falls into the 7 bit range
even though manufacturers and sales people might have you believe
otherwise. If you have an 8 bit board why not use a 8 bit or higher
camera.





From: DVCCO-at-aol.com
Date: Sun, 19 Mar 1995 03:54:55 -0500
Subject: Video Camera Selection Guide3of3

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Video Camera Selection Guide and DVC Product Release 3 of 3

* Sensitivities in the 1x10-4fc range at over 50% video. When other (IT)
type cameras barely see an image these DVC units are offering a perfect
image with extra gain to spare and no vertical line noise. Estimate
performance at about 5-6 ND filter better sensitivity than 768 type
interline transfer sensors.
* The only 10 bit digital camera with dual outputs RS-170 & RS-422 and at
real time video rates! 30 frames per second
* The only camera that has no sensor board. Sensor is physically mounted
to the front plate of the camera. Cooling above dew point can then be
done to have a truly quantitative camera in that temperature of sensor
can be lowered from room temperature or regulated at a constant
temperature or our -40C unit can be added.
* No need to cool the camera in most applications/ save the money.
* The DVC camera line was designed from the ground up to be a digital
output camera not a security camera. We use linear well regulated
multiple voltage linear power supply and solid C-Mounts with a 360
degree compression ring holding the c-ring firmly in place, not just a
little set screw that tends to loosen with time and strip out the
threads.
* 30dB of additional gain can be added for manual internal adjustable
operation accessible via a port from outside the camera, or external gain
and offset, or computer controlled gain! 30dB gain vs the others at 20dB
because the } 62db performance can fully utilize the 30db range. Where
with more than 20db on the IT types you just might end up with snow.
* Many other modifications can be done and our units do not come with AGC
unless you specify automatics. Automatics are for security cameras.
DVC will be responsive to your requirements from the end user to the OEM.
* Glass face plate removal to allow the user to easily go down to 300nm or
so. Customers with laser applications have reported response in the low
200nm range obviously at low quantum efficiencies.

The DVC camera line is excellent for any high signal to noise or low light
application and in real time!

DVC fits a niche between the too pricey and slow non real time digital only
video cameras and the security type cameras which don't offer enough of what
you need. If you like the resolution DVC can offer our flexibility in being
able to upgrade to the digital models is nice to have.
If any of you are interested in having a digital camera that works with your
Silicon Graphics Indy /Indigo 2 or Sun system, let us know.
DVC cameras are made in the USA and have a two year warranty.

* Three models of monochrome camera which are all upgradable to the DVC-10.
DVC-0A - RS-170 only with DB-37 connector for remote gain and upgradable
to the digital output RS-422 versions offering both
outputs.
DVC-8 - 8 bit RS-422 digital with RS-170 simultaneously.
DVC-10 -10 bit RS-422 digital with RS-170 simultaneously.

*** Below you will find a list of RS-422 digitizer boards that are compatible
to the DVC camera line, others are in the works.

****** Compatible Digitizers for RS-170 analog monochrome video are any
manufacturer. RS-170 is standard, typically from a BNC connector.
Does your board have capability to process RS-170 at 10 bits?
The DVC analog output will offer this. Remember if running 10 bit analog
RS-170 to a 8 bit board you will only see 8 bits.

Some of these boards have either 8 bit or 10 bit and higher capability.
*Compatible digitizers for RS-422 digital video that are
plug and play with the DVC digital camera line are listed below:

Manufacturer A-Z Platform Board Name/Model
Alacron IBM Alacron
Bit Flow IBM/MAC Raptor-VL
Coreco IBM F-64, OC-500
Datacube IBM MV-200
Dipix IBM P-360, XPG-1000
Epix IBM 4 Meg, Model 12
EDT SUN S-bus EDT
Hyperspeed IBM Hyperspeed
Imaging Technology IBM 15040, AFG, MFG
Imagraph IBM HI-Def
Matrox IBM Magic, 1280, 640, L/C
MuTech IBM 10 bit analog video in
Precision Digital (PDI) IBM/MAC IMAXX-PCI, SD
Univision IBM Piranha

Let DVC know if you wish, the following information:
Which digitizer board you are using or would like to use, or need help on.
If you have interest in the PCI bus
IBM, MAC, Sun, or SGI Indy or Indigo2 computer used
If DVC can help answer some of your technical concerns.
If your interest is analog or digital or both
If your interest is in tunable electronically selectable filters call DVC.

*****************************************************************************
***New DVC Camera Release estimated 4/95 will be the following:
Same features as above except now the digital RS-422 can be programmed or
reprogrammed for the VINO digital bus on the
((( SGI- Silicon Graphics Indy/Indigo 2 workstations)))
Imagine being able to move from SGI to RS-422 digital compatibility
with simultaneous RS-170 analog video!
Info on our cameras will be on the SGI Web soon.
*****************************************************************************

Feel free to e-mail, fax, or call DVC for more information.
If DVC can not reach you, we can not help you, so we chose to put this info
on
the net in hopes of helping anyone that needs it with a cost effective
research
grade video camera product that we feel, has a definite focus to this
microscope group. DVC has heard many of your problems and can be helpful,
and
possibly offer a solution. Stay in touch.

Sincerely,

Richard Klotsche
DVC Company
e-mail: dvcco-at-aol.com
phone: (619) 444-8300
fax: (619) 444-8321









From: m.dickson-at-unsw.edu.au (Melvyn R. Dickson)
Date: Mon, 20 Mar 1995 14:44:14
Subject: Re: Int'l MAS Conf??

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In article {n1416923531.369-at-mse.engin.umich.edu} "Wil Bigelow" {Wil_Bigelow-at-mse.engin.umich.edu} writes:
} Date: 14 Mar 1995 16:31:43 -0400
} From: "Wil Bigelow" {Wil_Bigelow-at-mse.engin.umich.edu}
} Subject: Int'l MAS Conf??

} Subject: Time: 4:23 PM
} OFFICE MEMO Int'l MAS Conf?? Date: 3/14/95

} I have heard rumors that there will be an international microbeam analysis
} meeting in Australia early in 1996. Does anyone know if
} this is so; and if it is, do you have any information about when and
} where it will be?

The meeting is the first meeting of the International Union of Microbeam
Analysis Societies (AKA IUMAS).

It will be held at the University of Sydney, Sydney, NSW Australia for Feb 5
to Feb 9 1996 (Preceeded by workshops) in conjunction with our 14th E.M.
Conference and 9th LM symposium. All welcome. Warm weather, water, cool
beer. David Cockayne is the chair.

Information on the WWW at http://www.bio.uts.edu.au/em.html





From: kris-at-miat0.vein.hu
Date: Fri, 17 Mar 1995 21:08:19
Subject: Subscription request

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Message-Id: {9503201754.AA22869-at-miat0.vein.hu}





From: Calidris-at-ett.se (George Farrants)
Date: 20 Mar 1995 08:38:54 GMT
Subject: Digitising TEM negatives

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Digitising images

For low cost, high capacity, high resolution and high quality,
_nothing_ beats film!

ÒUnfortunatelyÓ if you want to do digital image processing on
micrographs then they must be digitised. As previous answers have
indicated there is a choice between flat bed scanners and CCD
cameras. ThereÕs no doubt that the flatbed scanner produces better
data, but the cost is very high. (In addition to purchase price you have
maintenance, and so on.). Bill TivolÕs first reply overestimated the
cost of the CCD camera solution. You can get away with well under
$10000 for camera, lens, stand and light box, a tenth of the price for
flat bed scanners.

Optronics made a rotating drum scanner some years ago, but I think
itÕs disappeared now. Does anyone know? What did/does it cost?

The Ektron scanner mentioned by Larry Allard is new to me. Does the
referenced article give details? Who makes it?

Lab quality CCD cameras often have a geometric distortion between x
and y directions of a few percent. This must be measured and
corrected for in software. Another limitation is nonlinearity of the
response with OD, if you are planning on intensity measurements.
Again, it must be measured (with a stepwedge of standard ODs) and
corrected for.

CCD cameras have problems with ED patterns, as Bill also pointed
out. The very sharp spots with very high gradients cause trouble. I
know of some people who are using CCD camera for ED digitising,
maybe they can comment in the List about performance.

Another solution is to miss out film and have the CCD camera on the
microscope. Horribly expensive, but it has certain advantages. Is
anyone digitising ED patterns in this way, and what sort of
performance are they getting? I know of one group who is doing so,
with success. Are the specifications of on-line CCD cameras so much
better than lab ones?

As my old dad used to say ÒYou get what you pay forÓ when
confronted with The Sun newspaper at 20p and The Times, at 30p.

Yours,
George Farrants






From: sje-at-po.CWRU.Edu (Steven J. Eppell)
Date: Mon, 20 Mar 1995 10:13:55 -0500
Subject: networking a printer

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Message-Id: {199503201513.KAA19483-at-robin.INS.CWRU.Edu}


I would like to thank everyone that replied to my query concerning
networking a Tektronix Phaser IISDX dye sublimation printer.
Following is a summary of the problem and the responses.

I wished to put the printer on a Novell network so that it could be
accessed from macs, PCs, and Unix boxes. I had purchased Tektronix's
Internal Ethertalk card (~$650).

I was told by several listserver users and multiple people at Tektronix
that this was absolutely impossible to network the printer under a Novell
server using the Ethertalk card. Tektronix put me on to a company in
California called Milan that makes a network interface box that should
have allowed me to use the parallel port of the printer as the network
port. The cost of this box was ~$850.

I was told by several listserver users that it was trivial, no problem, plug
it in and it works regarding hooking the printer up to the network.

Finally, I convinced my network administrator to set up a que on one of
the network servers and to activate a faceplate so that I could connect
my printer to the net. The result .... Novell works fine with Tektronix's
Ethertalk card. I have printed from all three platforms and they all work.

The moral of the story: Keep asking your question until you get the
answer you want to hear!

p.s. For all of those highly experienced network users who told me in no
uncertain terms that I was a fool for not checking with my network
administrator before purchasing the printer .... Our local network here
has, conservatively speaking, 5,000 users. The network administration
is organized with a flowchart of personnel that would make the Ex-
Soviet politburo blush. I did try to go through logical channels before
making the purchase and was told, "we won't guarantee that it will work,
the best we can offer is for you to buy the printer and we'll test it out
when it gets here." I suppose this is probably an indication of what the
future of networking will be (once everybody's LAN gets so huge).




From: Jay Jerome :      jjerome-at-isnet.is.wfu.edu
Date: Mon, 20 Mar 1995 17:08:58 -0500 (EST)
Subject: CCD Guide

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Sorry for wasting the bandwidth, but last week a 3 part information guide
on a particular ccd camera was posted to this forum or the confocal
microscopy list server. A disk crash lost my
copy of this posting as well as the address of the sender before I had a
chance to review it. If the sender could repost a copy directly to me, I
would appreciate it.
Again, sorry for cluttering your mailbox.


Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-isnet.is.wfu.edu *
**************************************************************





From: Glen Macdonald :      glenmac-at-u.washington.edu
Date: Mon, 20 Mar 1995 18:21:36 -0800 (PST)
Subject: Re: embedding media & thick sections .....

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X-Sender: glenmac-at-homer07.u.washington.edu

Well, I was cleaning my email folders on the mainframe and came across
this postponed message. Here it is, hope it can still be useful.

Regards,
Glen


There is always celloidin, its disadvantages are a lengthy embedding
process. It produces a lot of shrinkage in animal tissue, the plant
cell wall should resist gross shrinkage, but there may be vacuolization.

For material that has been stained prior to embedding you might try
embedding in an epoxy like Pelco's EMBed. You can cut up to 50 microns
by either a carbide tungsten knive or using a standard steel knife.
These are real knives, not those disposable blades. You also need a
solidly built microtome.
The carbide knife only needs a slow cutting stroke. The steel knife
needs an extremely slow stroke or you can watch the metal forming the
knife's edge be ripped away before your very eyes. A small tacking iron,
like those used for dry mounting photographs, can be clamped on the end of
the knife with a C-clamp. Heat the knife to about 50 deg. C and the
plastic will cut more easily.

Glen MacDonald
Hearing Development Laboratories RL-30
University of Washington
Seattle, WA 98195
(206)543-8360
glenmac-at-u.washington.edu


On Tue, 8 Nov 1994, Mike Folsom wrote:

} Folks -
}
} A quick question -
}
} I'm trying to embed plant material is some type of matrix that will
} allow me to make fairly thick sections, say 25 - 50 um.
}
} I've used paraffin and the tissue starts to fracture when section
} thickness gets greater than 20 - 25 um. Frankly besides embedding
} the tissue in plastic and then grinding it down I'm not sure
} what else to do.
}
} I'd appreciate any suggestions -
}
} Michael
}
} _______________________________________________________________________________
} M.W.Folsom/Biology/UNM/Albuquerque,NM~87131/505.277.4277/mwfolsom-at-hydra.unm.edu
}
}





From: Stuart.McClure-at-adl.soils.csiro.au (Stuart McClure)
Date: Tue, 21 Mar 1995 14:20:01 +0900
Subject: Video Camera Selection Guide1of3

Contents Retrieved from Microscopy Listserver Archives
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Message-Id: {199503210352.AA02291-at-shrike.adl.soils.csiro.au}
X-Sender: mcc332-at-shrike.adl.soils.csiro.au
X-Mailer: Windows Eudora Version 2.0.3
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Jay Jerome {jjerome-at-isnet.is.wfu.edu}

} Date: Sun, 19 Mar 1995 03:52:28 -0500
} From: DVCCO-at-aol.com
} To: Microscopy-at-aaem.amc.anl.gov
} Subject: Video Camera Selection Guide1of3
}
} Subject: Guide to Choosing A CCD Video Camera With New DVC Product Release
} And Digitizer Selection Guide For Benefit of Microscope Users Group
}
} Monochrome Digital And Analog Video Camera Line By DVC Company
} Part 1 of 3 due to capacity limitations of e-mail
} *****************************************************************
} With all due respect: If anyone is offended by a guide to choosing a
} CCD video camera with a new product release that is
} applicable for the users in this group ( HIT YOUR DELETE KEY NOW! )
} There are some users that might appreciate this information.
} (This was done with good intentions) so any providers of negative feedback
} have been given the DELETE KEY OPTION and need not waste net time, thank you.
} Valid questions are appreciated and I will try to answer them relative to
} the volume I receive. Included is list of RS-422 digitizer boards in part 2
} and 3.
} * Please address any inquiry directly to dvcco-at-aol.com, not the main file
} server.
} *****************************************************************
} Dear Microscope Users,
}
} I wish to share some data with you on analog and digital monochrome cameras
} that I thought you might be able to make use of when reviewing your present
} or future camera situation. Some of the below information you might be aware
} of, but others might not. If anyone belongs to other pertinent groups and
} believes this information to be of benefit to that particular group we would
} appreciate you letting us know of them.
}
} Monochrome cameras offer much superior gray level and resolution performance
} along with higher signal to noise specs than the typical color versions.
} Everyone has different needs and the data below is for researchers who have
} decided to utilize the benefits of monochrome cameras. Adding pseudo colors
} to the many more gray levels attainable with a monochrome camera might be a
} option for you, or adding a tunable liquid crystal filter in line with the
} camera for serial Red, Green, Blue could also be a option if accurate
} correlation of the pixel is important. For monochrome applications a
} electronic tunable filter exists that is very selective with 5,7,10,15,20
} ,35 or 50nm band pass filters from 450 to 1050 nm range tunable
} electronically!
} See 2 of 2 for info on tunable filters.
}
} Some things to look for in a research grade monochrome camera are the
} following:
} * The highest signal to noise possible in a real time 30 frames/sec. unit.
} Signal to noise is the ultimate measure of a camera and no amount of
} manipulation, bells and whistles, or 20 different knobs to adjust, will
} change the bottom line which is the amount of gray levels obtainable.
} * No geometric distortion- CCD cameras offer basically no distortion while
} any tube camera gives you distortion in the } 5% range. My opinion is
} that to use a tube camera where a ccd unit could be substituted is just
} qualitative, not quantitative microscopy.
} * No dead pixels on your sensor and lowest pixel variation with no false
} fill-ins of pixels with data from the pixel next to it by use of memory
} circuits due to imperfections in the manufacturing process.
} Different types of CCD cameras have their uses.
} The 2 main types are frame transfer (FT) and interline transfer (IT)
} types for microscopy. CID types are also out there but have uses more in
} machine vision applications.
} Interline transfer sensors have large gaps between the pixels due to the
} construction. They offer 100% fill factor by the use of plastic
} micro lenses that help their sensitivity due to smaller well capacity,
} but still have the ( same smaller wells.) Plastic does not offer good
} performance down in the UV range below 400nm, and have other limitations
} at the near IR range. 1/3 format sensors have even less well capacity
} and I feel are only produced to provide better yields/profit for the
} manufacturers for security applications.
}
} Frame transfer sensors which DVC uses have dynamic range of 70dB to start
} and have no dead pixels and no false fill-ins. The wells are very
} large and the dark current is {30 electrons at 25C, room temperature.
} The pixels are contiguous, or within angstroms of electrical separation.
} Larger wells offer higher signal to noise. Double correlated sample and
} hold circuits built right into the sensor help reduce noise further on
} some frame transfer sensors. Frame transfer units offer one half the
} vertical
} resolution when doing integration and shuttering due the field transfer
} technique used.
} Spectral range with glass face plate attached 400-1100nm. Customers
} doing (laser) work have reported response in the low 200nm range with
} face plate removed and up to 1300nm.
}
} Typical bit vs signal to noise ranges:
} 7 bits = 44dB to technically 50dB but more like 54dB realistically
} 8 bits = 55dB to 61dB or 256 gray levels
} 10 bits = 62dB+ for 1024 gray levels. **10 bits offers 4 times the
} performance of an 8 bit camera.
} * Another words a camera specification of 50dB falls into the 7 bit range
} even though manufacturers and sales people might have you believe
} otherwise. If you have an 8 bit board why not use a 8 bit or higher
} camera.
}
}
}
---------------------------------------------------------------------------
Stuart G. McClure, | Post small : P.B.#2, Glen Osmond,
CSIRO Division of Soils, | Sth Australia, AUSTRALIA, 5064.
Adelaide Laboratories, | Post large : Waite Rd, Urrbrae,
SA, Australia | Sth Australia, AUSTRALIA, 5064.

Phone: (08) 303-8484 International use +61-8- instead of (08)
Fax: (08) 303-8550
Email: Stuart.McClure-at-adl.soils.csiro.au
---------------------------------------------------------------------------





From: Stuart.McClure-at-adl.soils.csiro.au (Stuart McClure)
Date: Tue, 21 Mar 1995 14:20:13 +0900
Subject: Video Camera Selection Guide2of3

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Message-Id: {199503210352.AA02295-at-shrike.adl.soils.csiro.au}
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Jay Jerome {jjerome-at-isnet.is.wfu.edu}

} Date: Sun, 19 Mar 1995 03:54:20 -0500
} From: DVCCO-at-aol.com
} To: Microscopy-at-aaem.amc.anl.gov
} Subject: Video Camera Selection Guide2of3
}
} DVC Video Camera Guide And Release 2 of 2
}
} Remember, if the camera is on the ragged edge of 8 bit performance and
} you want to add gain for more sensitivity, even the smallest amount of
} gain might put you in the 7 bit range if you we not there already.
} If you have 10 bit capability on your board why not use a 10 bit camera.
} * Also a camera specification of 60dB is not a 10 bit camera only an 8.
} * Also there are many types of noise in a sensor besides thermal noise.
} Cooling a sensor will reduce (thermal noise only) and allow you to
} integrate longer. I would double check the impression some make that
} when a 50db sensor is cooled it offers a better s/n of 10 or 12 bits vs
} just 7.
} The reduction of one type of noise (thermal) is all that cooling
} provides.
} * Other types of sensor noise besides dark current include photon, nyquest,
} shot, gain noise, and flicker noise etc.
} * The signal to noise of the sensor at room temperature is a good measure
} of what you can expect.
} ** Look at the quantum efficiency at the nm range needed.
} ** look at the spectral response of the sensor.
} ** There are different grades of sensors available relative to dead
} pixels and gray level variation on other manufacturers cameras.
} *Digital RS-422 eliminates pixel jitter for sub-pixel accuracy if it is
} needs versus the genlock action between the digitizer board and the
} camera as they try to lock to each other in the RS-170 analog mode.
} *Digital cameras of the higher end variety typically offer 7.5 frames/sec
} vs 30 (real time) or even less depending on the amount of pixels you need
} to process. If you wish to do real time imaging 30fps, 7.5fps will not
} do. Also for fluorescence the shortest duration exposure is preferred.
} * The camera has to have a good performance to cost ratio. At the low end
} there are plenty of security type cameras out there, but will they offer
} you the performance you require.
} * The big point here is that the higher the signal to noise, the quieter
} the signal, the more gain that can be added to the signal/ which adds noise
} as a result, but allows the user to get more sensitivity.
} Thus if you start with the quietest / highest signal to noise obtainable
} the better off you are when trying to get more gray levels with minimum
} sensitivity or more signal to noise allows more gain to be added to achieve
} a set sensitivity far superior to a signal to noise start point less than
} the DVC.
}
} The following features listed below are offered by the DVC camera line:
}
} General Statement: Analog video RS-170 focus
} *Most digitizer boards are typically 8 bits some go higher with digital
} RS-422
} input. With Dc's 10 bits of signal on the analog RS-170 video, this offers
} first of all more top end room for adding more sensitivity and still
} maintaining
} 8 bits/ 256 gray levels for the digitizer board.
} *You will see more digitizer boards with 10 bit capability on the analog
} RS-170
} input. What will you use as a real time 30 frame / sec camera to match that
} feature. With most ( real time ) cameras being in the 7 bit range, they
} would
} better qualify for security applications. There are some 8 bit cameras, but
} they only offer 256 gray levels with not much room to add gain for more
} sensitivity. The DVC camera line offers 10 bits or 1024 gray levels output
} for those boards along with simultaneous digital RS-422 on the digital models
} One of the digitizer boards with a 10 bit analog front end is Mutech. They
} are the only one presently that has that capability for now and others will
} follow soon.
}
} * Very high signal to noise } 62dB at .5 lux at 30fps/ real time
} * Frame transfer 1/2" format sensor with 755 horizontal by 484 vertical
} pixels, or 565 TV lines horizontal by 350 TV lines vertical resolution.
} * The only upgradable research camera with the researchers funding
} limitations in mind using the same high quality sensor on all 3 models.
} Upgradability from the RS-170 analog unit to the dual output 8 bit
} digital or to the dual output 10 bit digital models with RS-170 analog.
} (This allows the camera to grow with the researcher) and his budget.
} See part 3 of 3 next.
}
}
---------------------------------------------------------------------------
Stuart G. McClure, | Post small : P.B.#2, Glen Osmond,
CSIRO Division of Soils, | Sth Australia, AUSTRALIA, 5064.
Adelaide Laboratories, | Post large : Waite Rd, Urrbrae,
SA, Australia | Sth Australia, AUSTRALIA, 5064.

Phone: (08) 303-8484 International use +61-8- instead of (08)
Fax: (08) 303-8550
Email: Stuart.McClure-at-adl.soils.csiro.au
---------------------------------------------------------------------------





From: Stuart.McClure-at-adl.soils.csiro.au (Stuart McClure)
Date: Tue, 21 Mar 1995 14:20:17 +0900
Subject: Video Camera Selection Guide3of3

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Message-Id: {199503210352.AA02299-at-shrike.adl.soils.csiro.au}
X-Sender: mcc332-at-shrike.adl.soils.csiro.au
X-Mailer: Windows Eudora Version 2.0.3
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Jay Jerome {jjerome-at-isnet.is.wfu.edu}

} Date: Sun, 19 Mar 1995 03:54:55 -0500
} From: DVCCO-at-aol.com
} To: Microscopy-at-aaem.amc.anl.gov
} Subject: Video Camera Selection Guide3of3
}
} Video Camera Selection Guide and DVC Product Release 3 of 3
}
} * Sensitivities in the 1x10-4fc range at over 50% video. When other (IT)
} type cameras barely see an image these DVC units are offering a perfect
} image with extra gain to spare and no vertical line noise. Estimate
} performance at about 5-6 ND filter better sensitivity than 768 type
} interline transfer sensors.
} * The only 10 bit digital camera with dual outputs RS-170 & RS-422 and at
} real time video rates! 30 frames per second
} * The only camera that has no sensor board. Sensor is physically mounted
} to the front plate of the camera. Cooling above dew point can then be
} done to have a truly quantitative camera in that temperature of sensor
} can be lowered from room temperature or regulated at a constant
} temperature or our -40C unit can be added.
} * No need to cool the camera in most applications/ save the money.
} * The DVC camera line was designed from the ground up to be a digital
} output camera not a security camera. We use linear well regulated
} multiple voltage linear power supply and solid C-Mounts with a 360
} degree compression ring holding the c-ring firmly in place, not just a
} little set screw that tends to loosen with time and strip out the
} threads.
} * 30dB of additional gain can be added for manual internal adjustable
} operation accessible via a port from outside the camera, or external gain
} and offset, or computer controlled gain! 30dB gain vs the others at 20dB
} because the } 62db performance can fully utilize the 30db range. Where
} with more than 20db on the IT types you just might end up with snow.
} * Many other modifications can be done and our units do not come with AGC
} unless you specify automatics. Automatics are for security cameras.
} DVC will be responsive to your requirements from the end user to the OEM.
} * Glass face plate removal to allow the user to easily go down to 300nm or
} so. Customers with laser applications have reported response in the low
} 200nm range obviously at low quantum efficiencies.
}
} The DVC camera line is excellent for any high signal to noise or low light
} application and in real time!
}
} DVC fits a niche between the too pricey and slow non real time digital only
} video cameras and the security type cameras which don't offer enough of what
} you need. If you like the resolution DVC can offer our flexibility in being
} able to upgrade to the digital models is nice to have.
} If any of you are interested in having a digital camera that works with your
} Silicon Graphics Indy /Indigo 2 or Sun system, let us know.
} DVC cameras are made in the USA and have a two year warranty.
}
} * Three models of monochrome camera which are all upgradable to the DVC-10.
} DVC-0A - RS-170 only with DB-37 connector for remote gain and upgradable
} to the digital output RS-422 versions offering both
} outputs.
} DVC-8 - 8 bit RS-422 digital with RS-170 simultaneously.
} DVC-10 -10 bit RS-422 digital with RS-170 simultaneously.
}
} *** Below you will find a list of RS-422 digitizer boards that are compatible
} to the DVC camera line, others are in the works.
}
} ****** Compatible Digitizers for RS-170 analog monochrome video are any
} manufacturer. RS-170 is standard, typically from a BNC connector.
} Does your board have capability to process RS-170 at 10 bits?
} The DVC analog output will offer this. Remember if running 10 bit analog
} RS-170 to a 8 bit board you will only see 8 bits.
}
} Some of these boards have either 8 bit or 10 bit and higher capability.
} *Compatible digitizers for RS-422 digital video that are
} plug and play with the DVC digital camera line are listed below:
}
} Manufacturer A-Z Platform Board Name/Model
} Alacron IBM Alacron
} Bit Flow IBM/MAC Raptor-VL
} Coreco IBM F-64, OC-500
} Datacube IBM MV-200
} Dipix IBM P-360, XPG-1000
} Epix IBM 4 Meg, Model 12
} EDT SUN S-bus EDT
} Hyperspeed IBM Hyperspeed
} Imaging Technology IBM 15040, AFG, MFG
} Imagraph IBM HI-Def
} Matrox IBM Magic, 1280, 640, L/C
} MuTech IBM 10 bit analog video in
} Precision Digital (PDI) IBM/MAC IMAXX-PCI, SD
} Univision IBM Piranha
}
} Let DVC know if you wish, the following information:
} Which digitizer board you are using or would like to use, or need help on.
} If you have interest in the PCI bus
} IBM, MAC, Sun, or SGI Indy or Indigo2 computer used
} If DVC can help answer some of your technical concerns.
} If your interest is analog or digital or both
} If your interest is in tunable electronically selectable filters call DVC.
}
} *****************************************************************************
} ***New DVC Camera Release estimated 4/95 will be the following:
} Same features as above except now the digital RS-422 can be programmed or
} reprogrammed for the VINO digital bus on the
} ((( SGI- Silicon Graphics Indy/Indigo 2 workstations)))
} Imagine being able to move from SGI to RS-422 digital compatibility
} with simultaneous RS-170 analog video!
} Info on our cameras will be on the SGI Web soon.
} *****************************************************************************
}
} Feel free to e-mail, fax, or call DVC for more information.
} If DVC can not reach you, we can not help you, so we chose to put this info
} on
} the net in hopes of helping anyone that needs it with a cost effective
} research
} grade video camera product that we feel, has a definite focus to this
} microscope group. DVC has heard many of your problems and can be helpful,
} and
} possibly offer a solution. Stay in touch.
}
} Sincerely,
}
} Richard Klotsche
} DVC Company
} e-mail: dvcco-at-aol.com
} phone: (619) 444-8300
} fax: (619) 444-8321
}
}
}
}
}
}
}
---------------------------------------------------------------------------
Stuart G. McClure, | Post small : P.B.#2, Glen Osmond,
CSIRO Division of Soils, | Sth Australia, AUSTRALIA, 5064.
Adelaide Laboratories, | Post large : Waite Rd, Urrbrae,
SA, Australia | Sth Australia, AUSTRALIA, 5064.

Phone: (08) 303-8484 International use +61-8- instead of (08)
Fax: (08) 303-8550
Email: Stuart.McClure-at-adl.soils.csiro.au
---------------------------------------------------------------------------





From: Dr P. Echlin :      pe13-at-cus.cam.ac.uk
Date: Tue, 21 Mar 1995 08:36:37 +0000 (GMT)
Subject: Re: embedding media & thick sections .....

Contents Retrieved from Microscopy Listserver Archives
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microscopy list {Microscopy-at-aaem.amc.anl.gov}

With reference to embedding plant material for thick(ish) sections. We
routinely use LR White (London Resin White acrylic resin for plant
material and can certainly cut sections up to 15-20um. The nice thing
about LR white is that is like the British, tolerant and kind to
specimens and you need only dehydrate to 80% EtOH and still get godd
embedding. You can use all sorts of light microscopy stains on sections
cut from such material.

Greetings from a beautiful spring morning in Cambridge where all the
daffodils are in full bloom along the Backs.

Patrick Echlin

Multi-Imaging Centre
School of Biological Sciences


On Mon, 20 Mar 1995, Glen
Macdonald wrote:

} Well, I was cleaning my email folders on the mainframe and came across
} this postponed message. Here it is, hope it can still be useful.
}
} Regards,
} Glen
}
}
} There is always celloidin, its disadvantages are a lengthy embedding
} process. It produces a lot of shrinkage in animal tissue, the plant
} cell wall should resist gross shrinkage, but there may be vacuolization.
}
} For material that has been stained prior to embedding you might try
} embedding in an epoxy like Pelco's EMBed. You can cut up to 50 microns
} by either a carbide tungsten knive or using a standard steel knife.
} These are real knives, not those disposable blades. You also need a
} solidly built microtome.
} The carbide knife only needs a slow cutting stroke. The steel knife
} needs an extremely slow stroke or you can watch the metal forming the
} knife's edge be ripped away before your very eyes. A small tacking iron,
} like those used for dry mounting photographs, can be clamped on the end of
} the knife with a C-clamp. Heat the knife to about 50 deg. C and the
} plastic will cut more easily.
}
} Glen MacDonald
} Hearing Development Laboratories RL-30
} University of Washington
} Seattle, WA 98195
} (206)543-8360
} glenmac-at-u.washington.edu
}
}
} On Tue, 8 Nov 1994, Mike Folsom wrote:
}
} } Folks -
} }
} } A quick question -
} }
} } I'm trying to embed plant material is some type of matrix that will
} } allow me to make fairly thick sections, say 25 - 50 um.
} }
} } I've used paraffin and the tissue starts to fracture when section
} } thickness gets greater than 20 - 25 um. Frankly besides embedding
} } the tissue in plastic and then grinding it down I'm not sure
} } what else to do.
} }
} } I'd appreciate any suggestions -
} }
} } Michael
} }
} } _______________________________________________________________________________
} } M.W.Folsom/Biology/UNM/Albuquerque,NM~87131/505.277.4277/mwfolsom-at-hydra.unm.edu
} }
} }
}
}




From: jyoung-at-nsctoronto.com (James Young)
Date: Tue, 21 Mar 1995 08:57:34 +0500
Subject: NEW Address

Contents Retrieved from Microscopy Listserver Archives
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cemerson-at-kean.ucs.mun.ca, dhoyle-at-tic.ab.ca, bray-at-hg.uleth.ca,
beebed-at-ere.umontreal.ca, tyerman-at-cliff.path.queensu.ca,
MICROSCOPY-at-AAEM.AMC.ANL.GOV, Eric Kokko {kokko-at-EM.AGR.CA} ,
jvyoung-at-inforamp.net, lburton-at-cc.UManitoba.CA, asmith-at-aps.uoguelph.ca

We have our own Domain now. My personal address
is jyoung-at-nsctoronto.com

General address for service info and parts and prices is service-at-nsctoronto.com





From: NoahHadas-at-aol.com
Date: Tue, 21 Mar 1995 09:58:53 -0500
Subject: Micro-manipulation newsgroup?

Contents Retrieved from Microscopy Listserver Archives
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Hi all,

I'm a recent subscriber and am wondering if perhaps there is another
listserver that might be closer to my fields of interest, i.e.
micro-manipulation of cellular and sub-cellular particles either via mechanica
l or optical trapping techniques. If anyone knows of a group that is more
involved in these areas, I would appreciate if you could reply to me
directly.

Thanks,

Noah




From: Greg Begin :      GREGB-at-sales.lmt.com
Date: Tue, 21 Mar 1995 10:47:41 CST6CDT
Subject: Help finding Mr. Harold Kelly (Del if unknown)

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My appologies for clouding the Net with this traffic.

I have been attempting to get info to the following person,
if you can help, thank you in advance.

Person: Mr. Harold Kelly
address on the top of email: dy {hkelly-at-sgi73.wwb.noaa.gov

That is all I have on this person.

Thank you for your understanding and help on this.

Greg Begin - gregb-at-sales.LMT.com
3-21-95




From: greggk-at-acad.winthrop.edu
Date: Tue, 21 Mar 1995 12:52:05 -0500
Subject: Histology course idea

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WINTHROP UNIVERSITY Electronic Mail Message
Date: 21-Mar-1995 12:50pm EST
From: Kenneth Gregg
GREGGK
Dept: Biology
Tel No: 803-323-2111

TO: Remote Addressee ( _smtp%"microscopy-at-aaem.amc.anl.gov" )






WINTHROP UNIVERSITY Electronic Mail Message
Date: 21-Mar-1995 11:31am EST
From: Kenneth Gregg
GREGGK
Dept: Biology
Tel No: 803-323-2111

TO: Remote Addressee ( smtp%"microscopy-at-aaem.amc.anl.gov" )



Greetings,
My guess is that many members of the list teach a histology course at the
University level. I would like some comments on a method of teaching the
laboratory section of histology which we are considering. We find that many
students are rapidly bored when looking at tissue/organ sections in the light
microscope. They also seem to lack the ability to know when they have acquired
an acceptable level of expertise. As an antidote to this we are kicking around
the following idea -- and we would like to know if any members of the list have
tried similar approaches, and with what success?

We are thinking about having individual students prepare
computer-assisted-instruction lessons on topics such as epithelial tissues,
liver structure, etc. We would like to have the students prepare a hypertext
program for each topic. They would integrate the hypertext with captured images
from their light microscope observations as well as scanned diagrams and
electron micrographs which they could obtain from texts or photographs.
Students would be very much engaged in their own productions and could study
lessons prepared by other students. These programs could be used and improved
in subsequent years by other students. They could also be made available for
other institutions.

If you prefer, you may respond to me personally at
Greggk-at-Winthrop.edu

Thank you.





From: Michael Cammer :      cammer-at-aecom.yu.edu
Date: Tue, 21 Mar 1995 16:49:23 -0500 (EST)
Subject: query regarding light microscopy automation

Contents Retrieved from Microscopy Listserver Archives
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We are seriously interested in purchasing a setup for our Nikon Diaphot
that could be used for the following:
o quantitation of very low level fluorescence-- sensitive and linear
o but also useful for critical Nomarski applications such as microtubule
motility assays
o automation of filter wheels for multiple probes or fluorescence / phase
contrast image collection
o automation of stage moving, for instance for making mosaics automatically
or being able to move back to a specific field
o maybe Z motor and deconvolution, although the are not our main concerns
o ESSENTIAL: we are a multi-user facility so we need ease of operation

We are considering a Photometrics or Princeton Instruments cooled CCD camera.
We have looked at Oncor Imaging, I.P. Lab and have spoken to other
companies. For hardware, we have called companies that offer individual
parts (e.g. just the filter wheels) and, of course, have checked out Ludl.
We love NIH-Image, but want something does not require a large amount of
macro or other programming to get the hardware to work; basically, we want
plug and play.
Any suggestions or "stay away from this product at all costs" comments on
parts of systems or whole systems would be appreciated.
Thanks-
Michael Cammer
cammer-at-aecom.yu.edu






From: Weislaw Jablonski :      wis-at-lab.csl.utas.edu.au
Date: Wed, 22 Mar 1995 09:02:43 +1100
Subject: titanium in living tissue

Contents Retrieved from Microscopy Listserver Archives
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Hi there,
Could you help me with the problem of delineation of titanium in bone and
soft tissue.Any information and/or references would be a great help to me.
Study will involve the use of both SEM and EPMA, possibly FTIR as well.
Thank you all, Wis Jablonski , Email W.Jablonski-at-csl.utas.edu.au




From: Stephen A. Shaffer :      sshaffer-at-microdataware.com
Date: Saturday, April 1, 1995
Subject: San Francisco Micro. Soc. Meeting 4/1/95

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Place: Convene at Forensic Science Associates in Richmond,
Conclude at Oakland PD. (See contact information below for
reservations, maps, car pooling, and further details.)

Time: Convene by 10:00 am, Adjourn by 5:00 pm

Summary: This promises to be a most informative and interesting
meeting as we hear from some of the Bay Areas foremost
forensic microscopists, see some of the latest technology in
microscopy, and hear about some interesting forensic
applications of microscopy.

Agenda:

Forensic Science Associates, 10:00 am. Stephen A. Shaffer of
MicroDataware will introduce the topic of forensic science
in general and forensic microscopy in particular. Peter D.
Barnett of Forensic Science Associates will then discuss two
interesting cases involving forensic microscopy, and also
the role of the consulting (i.e., non-government)
microscopist in forensic cases. Pete will discuss cases
involving questions of ballot marking and alleged surgical
mistakes revealed by fibers in the nose! We'll conclude at
FSA by 11:30 or 11:45.

Bureau of Alcohol, Tobacco, and Firearms, 1:00 pm. After a brief
break for lunch at a Deli in Walnut Creek, we'll re-convene
at the ATF Laboratory where we will divide into two groups
to cover separate topics with two speakers. John Murdock
will explain and demonstrate the use of comparison
microscopy in firearms identification. John is the former
Director of the Contra Costa County Crime Laboratory and
also teaches criminalistics locally. He is an excellent
speaker and promises a fascinating discussion. Also at ATF,
we will meet with Robert Thompson who will demonstrate a new
technology for automated comparison microscopy. This
technology may revolutionize firearms work in criminalistics
laboratories by screening cases and suggesting most
promising comparisons for the human microscopist to perform.
We will adjourn from ATF by 2:30 to proceed to the Oakland
Police Department.

Oakland Police Department, 3:00 pm. At OPD, Diane Bowman will
discuss the use of microchemical testing procedures for the
identification of illicit drugs and narcotics, showing both
the utility and the beauty of these procedures for the rapid
identification of controlled substances. Mary Gibbons,
Director of the OPD Lab, will discuss a fascinating case
where microscopic particles of spray paint, identified and
compared by microscopy, were instrumental in unraveling a
suspect's story, leading to solution of a homicide. We will
aduourn from OPD by 5:00 pm.

At both ATF and OPD, each of our sub-groups will take turns with
each of the speakers so we all get to hear all of the
topics. Each topic will be covered in a presentation of
approximately 45 minutes duration.

Reservations Required!!! Because of the limited space available
within the microscopy labs we will visit, it may be
necessary to limit the number of people who attend. No more
than 20 can be accommodated so call Steve Shaffer ASAP to
reserve your space, receive maps to the locations, and to
coordinate with others on car pooling to the three sites we
will visit.

Contact: Stephen A. Shaffer at the numbers below. Don't delay,
call right away!

************************************************************
* Stephen A. Shaffer * Publishers of The Particle Atlas *
* MicroDataware * on CD-ROM. Developers of custom *
* 2894 Tribune Avenue * image and database software for *
* Hayward CA 94542-1637 * laboratories, specializing in *
* 1-510-582-6624 voice * light and electron microscopy. *
* 1-510-582-6624 fax * Email inquiries invited. *
* sshaffer-at-microdataware.com *
************************************************************





From: R.G.White-at-sci.monash.edu.au (Rosemary White)
Date: Wed, 22 Mar 1995 10:08:05 +1200
Subject: Re: embedding media & thick sections .....

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Plant material can also be embedded in butyl methyl methacrylate and
sectioned up to 20 um. Stick the sections down to glass slides with
polyethylenimine. The resin is removed with acetone before antibody
staining, but you don't need to remove it if staining with something small
and water-soluble like aniline blue.

____________________________________________________________
Rosemary White __ /
Department of Ecology _/ \__/ \
and Evolutionary Biology / \
Monash University / Australia \
Clayton, Victoria 3168 \ ____ /
phone 61-3-905 5670 \_/ \_*_/
fax 61-3-905 5613 __
email r.g.white-at-sci.monash.edu.au \/





From: Kris_Kavanau-at-dmcmail.ucsf.edu
Date: Tue, 21 Mar 1995 15:17:09 PST
Subject: Usage "Tracking" Programs

Contents Retrieved from Microscopy Listserver Archives
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Message-Id: {1995Mar21.151709.2878643829-at-dmcmail.ucsf.edu}
To: microscopy-at-aaem.amc.anl.gov (microscopy)

Subject: Time: 2:10 PM
OFFICE MEMO Usage "Tracking" Programs Date: 3/21/95
Hi everybody!
Does anyone have any usage tracking programs, or know where they might be
obtained? I am especially interested in a DOS program that is compatible
with Meridian software. Does anyone have any experience using tracking
programs? I am also interested in programs that are compatible with HP
and Macintosh acquisition/analysis software for flow cytometers (primarily
Becton Dickinson.)
Any suggestions or comments would be greatly appreciated. Thank you very
much!

__________________________________________________________
Kris Kavanau Internet:
kavanau-at-dmc.ucsf.edu
Manager, Lab for Cell Analysis Telephone: 415-476-2631
Division of Molecular Cytometry FAX: 415-476-8218
University of California
San Francisco, CA 94143
__________________________________________________________








From: Charles Garber
Date: Wed, 22 Mar 1995 07:43:48 EST
Subject: Titanium in living tissue [Question from Weislaw Jablonski on 3/21]

Contents Retrieved from Microscopy Listserver Archives
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-- [ From: Charles A. Garber, PH. D. * EMC.Ver #2.10P ] --

SPI Supplies

For TEM examination, we using the following procedure:

1] Pick up the unstained sections on a silicon dioxide coated TEM grid,

2] Gently etch (e.g. 10 seconds) the now supported section in a barrel
geometry reactive etcher (for example, the SPI Model Plasma Prep II)
using oxygen as the etching gas, which will immediately etch away all
traces of the organic matrix, leaving the inorganics dispersed, in
their original locations, on the silicon dioxide support film. Usually
these residues form a ghost type of outline of original cell shapes. I
don't know what bone might look like if etched this way.

The reason for the silicon dioxide support film is that it will not be
etched by the oxygen plasma, where as any kind of organic or
carbonaceous support film would otherwise be etched away.

The reason for the etching to remove the organic part of the sample is
to reduce to almost zero the Bremstrahlung radiation, thereby
increasing greatly the lower detection limits.

3] We have always found the TEM to be better for doing this kind of
analysis than SEM/EDS, mainly because of the higher resolution
possibilities.


Let me know if you would need further information. The making of the
silicon dioxide coated grids requires a bit of art but they are not all
that difficult to make, so long as you are patient.

Charles A. Garber, Ph. D.
PRESIDENT
SPI SUPPLIES
PO Box 656
West Chester, PA 19381-0656 USA

Ph: 1-(610)-436-5400
FAX: 1-(610)-436-5755
e-mail: sp-supp-at-cerf.net






From: delannoy-at-welchlink.welch.jhu.edu (Michael Delannoy)
Date: Wed, 22 Mar 1995 09:32:57 -0500
Subject: EM TECH POSITION

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The following position is available at the Johns Hopkins U. School of Medicine:

Electron Micrscopy Technician/19 hrs/wk PART-TIME:

Position will be responsible for semi- and ultra-thin sectioning on
glass and diamond knives. Standard grid prep and staining of sections;
maintenance
of electron gun parts (polishing cleaning etc.). Replenising of photo- and lab
chemical stocks, care of peripheral equipment and other related duties.
College degree, BS or equivalent and minimum two years experience.
Special
skills/knowledge of diamond knive use and maintenance, routine EM lab
operations.

All interested candidates should contact:
Lou Cote
(410) 955-0519
Job # M.3531.94

or

Mike Delannoy
(410) 955-1365





From: Maggy Piranian :      maggy-at-sparky2.esd.mun.ca
Date: Wed, 22 Mar 1995 13:31:51 -0330 (NST)
Subject: Re: Usage "Tracking" Programs

Contents Retrieved from Microscopy Listserver Archives
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Kris et al,
I use DIRECT ACCESS from Fifth Generation Systems. It keeps track of the
time, reqires a pssword and has the option of also requiring a project name.
This forces the issue with people who think it's better for me to chase
down their grant number then for them to remember, and makes billing much
easier. We also keep a log book for people to record problems and i modify
billings accordingly. When I came here this was already installed on the
XRD computer so I did no comparative shopping, & I don't know what else
is out there. Their address is
FIFTH GENERATION SYSTEMS INC
10049 n. Reiger Rd
Baton Rouge LA 70809
(504) 291-7221

Maggy Piranian
Memorial U of Newfoundland

On Tue, 21 Mar 1995 Kris_Kavanau-at-dmcmail.ucsf.edu wrote:

} Subject: Time: 2:10 PM
} OFFICE MEMO Usage "Tracking" Programs Date: 3/21/95
} Hi everybody!
} Does anyone have any usage tracking programs, or know where they might be
} obtained? I am especially interested in a DOS program that is compatible
} with Meridian software. Does anyone have any experience using tracking
} programs? I am also interested in programs that are compatible with HP
} and Macintosh acquisition/analysis software for flow cytometers (primarily
} Becton Dickinson.)
} Any suggestions or comments would be greatly appreciated. Thank you very
} much!
}
} __________________________________________________________
} Kris Kavanau Internet:
} kavanau-at-dmc.ucsf.edu
} Manager, Lab for Cell Analysis Telephone: 415-476-2631
} Division of Molecular Cytometry FAX: 415-476-8218
} University of California
} San Francisco, CA 94143
} __________________________________________________________
}
}
}
}
}




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Wed, 22 Mar 1995 11:30:37 -0600 (CST)
Subject: Sad News for the MSA Community

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Dear Colleagues,

We regrettably learned Monday, of the untimely death this last
Saturday of Mort Maser an individual whose efforts and contributions
to the Microscopy Community here in the US are well known and
valued by many of us.

Mort will be certainly missed and may I speak for the entire MSA
family in expressing our condolences to his family and friends.
For those of you who are interested I have gotten some details
concerning a memorial for Mort and detail those below.

..Nestor

-----------------------------------------------------------

In memory of Mort, his family is establishing the Morton D Maser
Scholarship Fund. Contributions can be sent to: Larry Maser,
P.O. Box EM, Woods Hole, MA 02543. Please designate your gift
to the Morton D Maser Scholarship Fund.

Memorial services celebrating Mort's life are being planned for the
summer months. Details will be announced as they are finalized.

---------------------------------------------------------------

Morton D "Mort" Maser, President of Woods Hole Educational
Associates, and Executive Secretary to Council of both the
Microscopy Society of America and the Histochemical
Society, died on Saturday, March 18, 1995 at the age of 60.

He earned his A.B. from the University of Pennsylvania in 1955,
and his Ph.D. in biophysics from the University of Pittsburgh in
1962. He held research and faculty positions at the Mellon
Institute, Harvard University, the Millard Fillmore Hospital,
Northeastern University, Creighton University, and the Marine
Biological Laboratory.

He developed roughly 100 short courses in electron microscopy and
related fields for scientists and technicians, and made more than
50 contributions to peer-reviewed publications on electron
microsopy and related topics.

He was a member of several professional societies including
serving as Chair of EMSA's Education Committee; past President,
Director of the New England Society for Electron Microscopy; the
American Association for the Advancement of Science; American
Society of Cell Biology; and the International Society for
Stereology.

His research has spanned the sciences from electron microscopical
techniques, to computer sciences, to software systems.

He is survived by his wife, Naomi, children Larry of Forestdale,
Massachusetts and Jill, of Philadelphia, and grandson, Benjamin.

-------------------





From: fskarl-at-goodyear.com (Frank Karl)
Date: Wed, 22 Mar 1995 13:06:15 -0500
Subject: Information wanted: Flatbed transparency scanners

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X-Sender: t456b15-at-rds163
Message-Id: {v01510101ab9615dcdabe-at-[163.243.13.93]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Dear Folks'

I was following the discussion on flatbed scanners with great interest, but
I need to find some vendors to request additional information. The
following units were mentioned: Umax PowerLink
AGFA Arcus
Perkin-Elmer
and
Optronics

Does any one have address, phone number or E-mail address for these or
other vendors?

Thanks for the assistance....Frank


Frank Karl fskarl-at-goodyear.com
Goodyear Tire & Rubber Co. Voice 216.796.7818
Analytical Services - Dept 415B Fax 216.796.3304
142 Goodyear Blvd
Akron, OH 44305
U.S.A.









From: M.V. Parthasarathy :      mvp2-at-cornell.edu
Date: Wed, 22 Mar 1995 14:41:51 -0400 (EDT)
Subject: RE: replacement for lactophenol

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X-NUPop-Charset: English

In message Mon, 20 Mar 95 14:23:11 EST, forbes-at-cip.org.ec writes:
We would greatly appreciate
} suggestions on other ways to clear and fix this tissue that do not
} involve lactophenol or other products which require special
} disposal facilities, which currently do not exist in Quito.
*******

We routinely use 1 M NaOH to clear flowers to check in vivo pollen growth
after Aniline Blue staining (for fluorescence). You might also want to try
a clearing protocol specifically designed for fungal infected leaf tissue
such as the one in the following publication:

AU HIGNIGHT-K-W. MUILENBURG-G-A. VAN-WIJK-A-J-P.

TI A CLEARING TECHNIQUE FOR DETECTING THE FUNGAL ENDOPHYTE ACREMONIUM- SP

IN GRASSES

SO BIOTECH HISTOCHEM

68 (2). 1993. 87-90.

JT BIOTECHNIC & HISTOCHEMISTRY.

KW FESTUCA-ARUNDINACEA FESTUCA-OVINA LOLIUM-PERENNE BRIGHT FIELD

MICROSCOPY ANALYTICAL METHOD

AB Leaf tissue of tall fescue Festuca arundinacea Schreb., hard fescue

Festuca ovina L., red fescue Festuca rubra L. and Perennial ryegrass

Lolium perenne L. was stained with rose Bengal or aniline blue to

detect the presence of the fungal endophyte Acremonium sp.. Specimens

were cleared using methyl salicylate, an optical clearing agent, and

viewed using bright field microscopy. Tissue was preserved as dried

tissue or stored in 70% aqueous ethyl alcohol before staining and

clearing. Tissue was observed at 2, 4 and 12 weeks following clearing

to check for stain retention. Staining with rose Bengal was inferior to

aniline blue when followed by the clearing agent methyl salicylate.

Fungal mycelia stained lighter with rose Bengal and were more difficult

to detect than mycelia stained with aniline blue. The results

illustrate the usefulness of combining staining and methyl salicylate

clearing for detecting fungal endophytes.

___________

Good Luck!

*************************************************************************
M.V. Parthasarathy
Professor of Plant Biology
Section of Plant Biology
228 Plant Science Building
Cornell University, Ithaca, NY 14850
Telephone: (607) 255-1734
Fax: (607) 255-5407
E-mail: mvp2-at-cornell.edu
***********************************************************************




From: nina allen :      allen-at-wfu.edu
Date: Wed, 22 Mar 1995 16:56:34 -0500 (EST)
Subject: Re: query regarding light microscopy automation

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Have you tried calling Universal Imaging (you mentioned some of the other
competitive companies). They are at 610-344-9410. Their new metamorph
system will do just about everything you requested...and works well with
a cooled CCD.
Nina Allen




From: Strucural Biology Unit :      MICROSCOPY-at-SBSNOV1.AUCKLAND.AC.NZ
Date: Thu, 23 Mar 1995 10:03:42 GMT+1200
Subject: RE:35mm film in TEM

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} Date sent: Mon, 27 Feb 1995 09:30:43 -0500 (EST)
} From: YANGA-at-NCCCOT.AGR.CA
} Subject: RE:35mm film in TEM
} To: microscopy-at-aaem.amc.anl.gov

} We use Eastman motion picture film (5302 fine grain release
} positive film) in Philips EM300 in the past and currently in
} Zeiss EM902 without any problem.
}
} Ann Fook Yang

We use copex PET10 in our CM12 and 301. It doesn't outgase much, is
very thin so is easy to handle and has not sprocket hole to interfere
with the images (depending onthe format).

Terry





From: fskarl
Date: Wednesday, March 22, 1995 1:06PM
Subject: Information wanted: Flatbed transparency scanners

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Dear Folks'

I was following the discussion on flatbed scanners with great interest, but
I need to find some vendors to request additional information. The
following units were mentioned: Umax PowerLink
AGFA Arcus
Perkin-Elmer
and
Optronics

Does any one have address, phone number or E-mail address for these or
other vendors?

Thanks for the assistance....Frank


Frank Karl fskarl-at-goodyear.com
Goodyear Tire & Rubber Co. Voice 216.796.7818
Analytical Services - Dept 415B Fax 216.796.3304
142 Goodyear Blvd
Akron, OH 44305
U.S.A.









From: delannoy-at-welchlink.welch.jhu.edu (Michael Delannoy)
Date: Thu, 23 Mar 1995 09:46:25 -0500
Subject: EM TECH POSITION

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} To: microscopy-at-aaem.amc.anl.gov
} From: delannoy-at-welchlink.welch.jhu.edu (Michael Delannoy)
} Subject: EM TECH POSITION
}
} The following position is available at the Johns Hopkins U. School of Medicine:
}
} Electron Micrscopy Technician/19 hrs/wk PART-TIME:
}
} Position will be responsible for semi- and ultra-thin sectioning on
} glass and diamond knives. Standard grid prep and staining of sections;
maintenance
} of electron gun parts (polishing cleaning etc.). Replenising of photo- and lab
} chemical stocks, care of peripheral equipment and other related duties.
} College degree, BS or equivalent and minimum two years experience.
Special
} skills/knowledge of diamond knive use and maintenance, routine EM lab
operations.
}
} All interested candidates should contact:
} Lou Cote
} (410) 955-0519
} Job # M.3531.94
}
} or
}
} Mike Delannoy
} (410) 955-1365
}





From: almonte-at-medcolpa.edu
Date: Thu, 23 Mar 1995 09:51:36 -0400
Subject: Cy5 &Cy3 background staining?

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Dear Friends,
As you all may remember, I was having crossing over problem between
FITC & Rhodamine. I followed the suggestion that some of you gave me and
used Cy5 (1.4 mg/ml) 1:100 and 1:400 and Cy3 (1.4 mg/ml) same dilution. I
did a control and I got a lot background staining. Any suggestion?
My next step will be to check my filters as some of you suggested.
Nichole Dutton suggested that before applying primaries antibodies
to block for nonspecifics with 20% horse serum solution, and use 1% bovine
serum albumin in my diluents. Nichole, I'd like further information on
this.
Dave, also suggested using different blocking reagent (BSA, milk,
gelatin, serum.) I'd like to learn more about this possibility, Dave.
Thanks,
Ciprian

__________________________________________________________
Ciprian Almonte
Medical College of Pennsylvania
Dept. of Anatomy and Neurobiology E-mail: almonte-at-medcolpa.edu
3200 Henry Ave. Voice: (215) 842-4081
Philadelphia, PA 19129 Fax: (215) 843-9082
__________________________________________________________







From: tivol-at-tethys.ph.albany.edu
Date: Thu, 23 Mar 1995 11:09:55 EST
Subject: Re: Information wanted: Flatbed transparency scanners

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Dear Frank,
There was a used Perkin-Elmer scanner available, which I heard about
from the microscopy list. I don't remember the company name, since we didn't
have the money to get the scanner. They sent me a write-up with specs, but I
don't know whether I still have it. Perhaps you or Nestor can search the ar-
chives for the posting. Good luck.
Yours,
Bill Tivol




From: David Hwang-RA2169 :      david_hwang-ra2169-at-aprdlgtr.sps.mot.com
Date: 23 Mar 95 11:27:24 U
Subject: Position Available for FIB

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Message-Id: {9503231732.AA19857-at-mogate}

REGARDING Position Available for FIB Engineer
Position available for a FIB engineer to join a team working on process
development and failure analysis of ULSI circuits in Motorola, Advanced
Products Research and Development Laboratory, Austin, Texas. The engineer will
be responsible for the setup and operation of a focused-ion-beam facility. The
candidate must have hands-on experience on FIB. Qualified candidates please
send resumes to

ra2169-at-email.mot.com

or mail to

David Hwang
Motorola
APRDL, K-10
3501 Ed Bluestein Boulevard
Austin, TX 78721







From: Lata Prabhu 512-356-7894 :      Lata.Prabhu-at-SEMATECH.Org
Date: Thu, 23 Mar 1995 11:51:00 -0600 (CST)
Subject: FIB TECHNIQUES

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MR-Received: by mta GATEV3; Relayed; Thu, 23 Mar 1995 12:19:38 -0600 (CST)
Alternate-recipient: prohibited
Disclose-recipients: prohibited


Hi,
I am a TEM sample prep technician and is currently preparing
samples by self supportive dimpling, tripod and acid etch(mainly
for planview)techniques. I am interested in new methods out there
being developed and used successfully. Is anyone out there
preparing samples using FIB(Focus Ion Beam) as a tool.We have used
it once in a while to thin our samples prepared with the wedge
technique in the past, but would like to know more.We own a FEI800,
but is currently used extensively for SEMs. Also any tips,and/or
problems to avoid, associated with the sample prep(especially in
the milling dept.) are most welcome. Most of my contamination
problem occurs in the ion-milling process.
Thanks,
Lata Prabhu







From: Lata Prabhu 512-356-7894 :      Lata.Prabhu-at-SEMATECH.Org
Date: Thu, 23 Mar 1995 14:45:00 -0600 (CST)
Subject: returned message

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Message-Id: {9503231940.AA07932-at-riker.ml.wpafb.af.mil}


Just checking to see if the message is getting through, my earlier
one bounced back,
Lata





From: Doug Davis :      doug_davis-at-maillink.berkeley.edu
Date: 23 Mar 1995 13:23:56 -0800
Subject: Rotary shadowing of protein

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Message-ID: {n1416157433.59430-at-maillink.berkeley.edu}

Hello all- I am looking for recommendations for evaporation material used in
rotary shadowing of proteins. I am referencing the text "Electron Microscopy
in Molecular Biology", a practical approach; ed. by Sommerville and Scheer.
The method described uses a Balzers electron beam gun to evaporate
tungsten/tantalum. We do not have such a gun but plan to use a standard
thermal evaporation.
Anyone out there have experience/recommendations for evaporation metal,
e.g., platinum, tungsten, palladium, carbon/platinum for finer grains?

Thanks,
Doug Davis
EML Berkeley
(510) 642-2085
doug_davis-at-maillink.berkeley.edu





From: Doug Davis :      doug_davis-at-maillink.berkeley.edu
Date: 23 Mar 1995 15:09:49 -0800
Subject: Job Vacancy Listing

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Message-ID: {n1416151173.33639-at-maillink.berkeley.edu}

Subject: Time: 2:52 PM
OFFICE MEMO Job Vacancy Listing Date: 3/23/95

Job opening: Staff Research Associate II
Electron Microscope Laboratory
University of California at Berkeley
$29,200 per annum, 80-100% appointment
Job listing #03-345-30/SL
closing date: 4-14-95
Contact the Office of Employment
Room 7-G
2200 University Ave., Berkeley, CA 94720
(510) 642-1011 general info

Operate and maintain an SEM. Train users in SEM technique, including specimen
preparation. Learn operation of JEOL 9000 freeze-fracture machine and train
and supervise others in its use. Prepare samples for immunoelectron
microscopy (TEM) and occasionally supervise TEM users. Operate cryofixation
equipment for EM sample preparation . Train users in darkrom technique and
supervise use of lab computers. Qualifications: Experience in cryofixation
and immunocytochemical methods. General laboratory skills (preparation of
buffer solutions, operating pH meters etc.). Familiarity with using computer
programs (word processing, spreadsheets). Familiarity with electronic
equipment and its routine maintenance. Darkroom experience. Ability to work
independently.

Personnel will not send out job applications. If the applicants cannot come
to the personnel office to pick up an application they can send a:

1. Cover Letter referencing the job number (03-345-30)
2. Resume

The cover letter and resume should be mailed to:

University of California Berkeley
Berkeley Campus Employment Office
Room 7-G (Ground Floor)
2200 University Ave.
Berkeley, Ca. 94720







From: Lackhlan Ritchie :      etpsemra-at-zonk.geko.com.au
Date: Fri, 24 Mar 1995 13:18:15 +1000 (EST)
Subject: Please UNsubscribe us

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Please UNsubscribe us for this mailing list.
Thank you.




From: IAN HALLETT :      ihallett-at-marccri.marc.cri.nz
Date: Fri, 24 Mar 1995 15:33:33 GMT+1200
Subject: Insect gut fixation

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A message forwarded from my colleague Paul Sutherland

Has anyone got a good fixation method for insect gut tissue which has
a thick peritrophic membrane. At present I am using 2%
paraformaldehyde, 2.5% glutaraldehyde in 0.1M phosphate buffer pH
7.2 and a post fix in 1% osmium tetroxide. With this fix the
microvilli are not well preserved.


Ian Hallett


Ian Hallett
HortResearch
Mt Albert Research Centre
Private Bag 92 169
Auckland, New Zealand
Fax 64-9-815 4201
Telephone 64-9-849 3660




From: Mike Gregory :      mgregory-at-pixie.udw.ac.za
Date: Fri, 24 Mar 1995 09:09:21 +0200 (SST)
Subject: ATP-ase for LM and TEM

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We are trying to localise enzyme sites (ATP) on the plasmalemma of salt
glands in halophytic grasses. Has anyone out there got a method(s) for
ATP-ase at the LM level for glut. fixed tissue already embedded in
historesin. Also, methods for ATP-ase in samples for TEM.

Thanks

Yogis






From: Fangl-at-fpms.fpms.ac.be
Date: Fri, 24 Mar 1995 15:18:14 +0100
Subject: help

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Hi, does anyone know FTP site for program of electron diffraction analysis ?
Thanks for your help!
L.FANG




From: tivol-at-tethys.ph.albany.edu
Date: Fri, 24 Mar 1995 12:36:55 EST
Subject: Re: Rotary shadowing of protein

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Dear Doug,
I suggest you look up articles by R.P. Apkarian. He has a Cr coating
method which produces extremely fine grains. He told me of a detailed paper
submitted to Scan. Microsc. Intl. which should have come out in 1994. I have
a few of his papers, but not that one. Good luck.
Yours,
Bill Tivol




From: Dennis Lazof :      dlazof-at-email.unc.edu
Date: Fri, 24 Mar 1995 14:02:20 -0500 (EST)
Subject: Re: Rotary shadowing of protein

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Please UNsubscribe me.

Thanks.




From: samso-at-tethys.ph.albany.edu
Date: Fri, 24 Mar 1995 17:19:03 EST
Subject: unsubscribe

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unsubscribe





From: MED_SCU-at-FRCU.EUN.EG
Date: Sat, 25 Mar 1995 10:35:56 +0000 (O)
Subject:

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First Intarnational Conference on Electron Microscopy
and
Advances in Research in Differcent Fields of Sciance
September 1995
Ismailia - Etap

Sponsored by
Electron Microscopy Center
Suez Canal University
Ismailia - Egypt

Special Topics of the conference:
1- Role of EM in diagnostic virology.
2- Role of EM in diagnosis of tumors cylulogy and urinary atones.
3- Role of EM in ultrastructure pathology of the lung (non neoplastic
conditions).
4- X-ray microanalysis: Applications particularly metalllurgical,
mineralogical, and biological.
5- Scanning EM of plants, animal, ineccts, and mineral material.
6- Study of biological macromolecules from their characteristic
electron diffraction patterns.
7- Skin pathology by EM.
8- Morphological ldentification of antigens by EM.
9- Different low temperature methods for biological EM.
10- Safety measures and maintenance needed for EM.

There will be an equipment exblbition in conjunction with this
meeting. Registration for foreigners will be US $ 150 inclusive of
full board during the time of the meeting.
For further information, contact the organizar:
Prof, Dr. Khalifa Ibrahim Khalifa
Electron Microscvpe Center
Suez Canal University
Ismailia - Egypt
Fax: (20) 64- 329478 ( phone and Fax number)
Fax: (20) 64- 333318 ( Phone and Fax number)

Massage from: sayed Mersal





From: Abdel-Salam Al-Drouby :      WMMAA-at-cardiff.ac.uk
Date: Sat, 25 Mar 1995 14:06:25 GMT
Subject: Is there a list digest?

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Hello,

Could someone please tell me how I can get a digest i.e. daily of the
emails sent on this list, if there is one.

Thank you,

Abdel

Abdel-Salam Al-Drouby
Dept of Medical Microbiology
University of Wales College of Medicine
Heath Park
Cardiff
CF4 4XN
Tel 744724




From: Gary Login :      glogin-at-bih.harvard.edu
Date: Sun, 26 Mar 1995 12:49:40 -0500
Subject: Re: Insect gut fixation using microwave methods

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Microwave irradiation has been shown to enhance the penetration of aldehyde into
insect and plant tissues and to improve fixation of these specimens for LM and
EM. Five references follow:

1. Lindley VA: A new procedure for handling impervious biological specimens.
Microsc Res Tech 21:355, 1992

2. Smid HM, Schooneveld H, Meerloo T: Microwave fixation of water-cooled insect
tissues for immunohistochemistry. Histochem J 22:313, 1990

3. Heumann HG: Microwave-stimulated glutaraldehyde and osmium tetroxide fixation
of plant tissue: ultrastructural preservation in seconds. Histochem 97:341, 1992

4. Login GR, Dvorak AM: Methods of microwave fixation for microscopy. A review
of research and clinical applications: 1970-1992. Prog Histochem Cytochem
27/4:1, 1994

5. Login GR, Dvorak AM: The Microwave Toolbook. A Practical Guide for
Microscopists. Boston, Beth Israel Hospital Press, 1994, 184








In message {7247D4029F-at-marc.cri.nz} "IAN HALLETT" writes:
} A message forwarded from my colleague Paul Sutherland
}
} Has anyone got a good fixation method for insect gut tissue which has
} a thick peritrophic membrane. At present I am using 2%
} paraformaldehyde, 2.5% glutaraldehyde in 0.1M phosphate buffer pH
} 7.2 and a post fix in 1% osmium tetroxide. With this fix the
} microvilli are not well preserved.
}
}
} Ian Hallett


GRL
glogin-at- bih.harvard.edu
Beth Israel Hospital - Department of Pathology
Telephone: 617-667-2034
Fax: 617-667-8676





From: Doug ext. 2470 :      bray-at-hg.uleth.ca
Date: Sun, 26 Mar 1995 19:18:55 MST
Subject: Recording CRT for Hitachi S-500

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Dear Listserver Audience:
The tektronix image recording crt (Current # CRTC818P4S) on our Hitachi S-500
scanning EM is no longer functional. We have temporarily replaced it with a
high resolution monitor, however this is not satisfactory due to the smaller
image. Effort at expanding the image have resulted in some edge distortion and
a loss of resolution.
Does anyone out there have a replacement tube that they wish to sell? If so,
please contact me at:

BRAY-at-HG.ULETH.CA

Thanks,
Doug Bray




From: Doug ext. 2470 :      bray-at-hg.uleth.ca
Date: Sun, 26 Mar 1995 20:14:55 MST
Subject: Recording CRT for Hitachi S-500

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Dear Microscopy Listserver Audience:
The tektronix image recording crt (#CRTC 818 P4S) on our Hitachi S-500 scanning
EM is no longer functional. We have temporarily replaced it with a high res.
monitor, however this is not satisfactory due to problems associated with the
small image produced by the shorter yoke on this crt. Attempts at expanding
the image electronically have resulted in some edge distortion and an unaccept-
able level of resolution loss.
Does anyone out there have a replacement crt of this type? If so, please
E-mail me at:
Bray-at-hg.uleth.ca

Thanks,
Doug Bray




From: kris-at-miat0.vein.hu (Kovacs Kristsf)
Date: Mon, 27 Mar 1995 08:53:22 -0500
Subject: Upgrading old EDS systems

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Dear Friends,

We have an 18 years old EDAX EDS system. The MCA and computer is slow and
unreliable. Does anybody has information on upgrading old EDS systems by
replacing the multichannel analyser and computer with an IBM PC or MAC? We
would like to keep it as cheap as possible.

Thanks!

Kris

Kristof Kovacs
Central Laboratory
University of Veszprem
Veszprem, P.O.Box 158
H-8201 Hungary
Phone: +36-(88)-421684
Fax: +36-(88)-426016
e-mail: kris-at-miat0.vein.hu





From: Keith Moulding :      MCMOULDK-at-usthk.ust.hk
Date: 27 Mar 1995 17:31:16 +0800
Subject: Re: upgrading old EDS systems

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Hello,

Yes, you can upgrade old EDS systems. There is a program called WinEDS.
Coupled with a plug in board for your PC, your detector and pulse
processor you get a Windows based quantitative system.

Sorry I do not have the address of the person who wrote/design the
system, however I will try and track him down (he is in Australia).

This may take a few weeks.


Keith Moulding
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
HKUST
MCPC,
Clear Water Bay,
Kowloon
Hong Kong.

FAX (852) 2-358-2451.




From: waheeschen-at-dow.com (Bill Heeschen 517-636-4005 Materials/ASL)
Date: Mon, 27 Mar 1995 09:21:08 -0500
Subject: Re: Upgrading old EDS systems

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Kris:

I would suggest a call to 4-pi analysis. Their product line emphasizes
upgrading existing EDS systems and works with NIH Image and DTSA (and others)

4-pi analysis
3500 Westgate Dr. Suite 403
Durham, NC 27707-2534
USA


phone:
Mike Sczyz
(919) 489-1757

fax:
(919) 489-1487

electronic mail:
go4pi-at-applelink.apple.com



Bill
==========================
Bill Heeschen/Analyt. Sci.- Mat. Char. -----
1897-F Building / The Dow Chemical Co. --- ---
Midland, MI 48667 U.S.A. --- D O W ---
phone: (517)636-4005 fax: (517)636-5453 --- ---
Email: waheeschen-at-dow.com -----
========================== R




From: jmcgee-at-lunatic.er.usgs.gov (Jim McGee)
Date: Mon, 27 Mar 1995 10:10:20 -0500
Subject: Re: upgrading old EDS systems

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The WinEDS system described by Keith Moulding is designed and marketed by
Paul Thomson:
Thomson Scientific Instruments
216 Drummond Street, Carlton, 3053,
Victoria, Australia
phone (03) 663 2738.
fax (03) 663 3680

I had the opportunity to demo this system at the New Orleans MAS/MSA. It
is an impressive package. This is not an endorsement, just a personal,
scientific opinion.

Jim McGee


U.S. Geological Survey
Reston, VA 22092





From: mullerw-at-rmslab.rockefeller.edu
Date: Mon, 27 Mar 1995 10:23:19 EST
Subject: Sending messages

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Dear Colleagues:

This is a test. I tried to resend a message to this address and it was retu
returned by the Rockefeller mail director. I couldn't resend it. Let's see if
this gets out.

Bill Muller





From: tvoiles-at-unlinfo.unl.edu (Todd Voiles)
Date: Mon, 27 Mar 1995 10:35:44 -0500
Subject: EDS Upgrades

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Message-Id: {199503271600.RAA27751-at-gate.sbbio.be}

Does anybody know how much the WinEds costs?

Seems to be a great disparity between the other packages I've looked at :

DTSA $800 and quirky
Flame $7000 and Fast


Anybody know of any pakages somewhere in the middle?
Center for Materials Research and Analysis
Central Facility for Electron Microscopy
University of Nebraska at Lincoln

Todd Voiles
tvoiles-at-unlinfo.unl.edu





From: BARRY PYLE :      umbbp-at-msu.oscs.montana.edu
Date: Mon, 27 Mar 1995 10:04:30 MST
Subject: Fluorescent in situ hybridization/Bacteria

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I am trying to do fluorescent in situ hybridization with bacteria directly on
Nuclepore polycarbonate membrane filters, and need to omit the alcohol series
that is usually included in these protocols. Does anyone have any F.I.S.H.
protocol that does not include the alcohol series? It doesn't need to be a
membrane filter method. I am, however, interested in any F.I.S.H. membrane
filter protocols. What types of membranes work best?

Thanks, Barry Pyle, Montana State University - Bozeman.




From: RYERSEJS :      RYERSEJS-at-SLUVCA.SLU.EDU
Date: Mon, 27 Mar 1995 14:06:37 -0600 (CST)
Subject: INSECT GUT FIXATION

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I don't know how thick you mean for the peritrophic membrane, but
I've had good results by injecting fixative into the gut lumen and letting
it work awhile before dissecting the gut out in fixative solution. This works well for lep guts. The fixative etc is described in Ryerse et al, Tissue and
Cell, 24:751-771 1992. See discussion of preventing midgut cell surface
blebbing on p 768 by injecting fix directly into the gut lumen. Good luck.
Jan Ryerse, Pathology, St. Louis University Med School, St. Louis, MO





From: Laurie Frederick :      frederick_laurie-at-vanlab.paprican.ca
Date: Mon, 27 Mar 1995 09:38:14 PDT
Subject: Re: Upgrading old EDS systems

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Message-Id: {MAILQUEUE-101.950327093814.384-at-vanlab.paprican.ca}
To: microscopy-at-aaem.amc.anl.gov

Hi,
For an economic EDX system set up to utilize your existing detector, I
suggest you contact the following:

Dapple Systems, Sunnyvale CA , (408) 733-3283
They supply a PC or Mac based system which I believe is passive
only( ie. does not take control of the beam)

4pi Analysis, Inc. Durham NC , (919) 489- 1757
They supply a Mac or Power Mac based system (with beam
control?)

I have only read their brochures not used the systems, but I think they
are worth a look.

Good luck,
Laurie

Kristsf Kovacs wrote:
}
} We have an 18 years old EDAX EDS system. The MCA and computer
is slow and
} unreliable. Does anybody has information on upgrading old EDS
systems by
} replacing the multichannel analyser and computer with an IBM PC or
MAC? We
} would like to keep it as cheap as possible.
}
} Thanks!
}
} Kris
}
} Kristof Kovacs
} Central Laboratory
} University of Veszprem
} Veszprem, P.O.Box 158
} H-8201 Hungary
} Phone: +36-(88)-421684
} Fax: +36-(88)-426016
} e-mail: kris-at-miat0.vein.hu
}
}

______________________________________________________________________
Laurie Frederick, A.SC.T. PAPRICAN
Corrosion Control Group 3800 Wesbrook Mall
The Pulp and Paper Research Vancouver, B.C.
Institute of Canada Canada V6S 2L9

Email: frederick_laurie-at-vanlab.paprican.ca
Tel: 604-222-3200 Fax: 604-222-3207




From: mullerw-at-rmslab.rockefeller.edu
Date: Mon, 27 Mar 1995 11:25:10 EST
Subject: Position available

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Dear Microscopy colleagues:

A fully-funded position is available in my laboratory for a postdoctoral
fellow or an advanced EM technician who can work independently.
I am looking for someone with experience in thin sectioning of mammalian
tissue culture cells and tissues. Specifically, this project involves immunoEM
analysis by post-embedding procedures (e.g. Lowicryl or L.R. White) and/or
ultrathin frozen sectioning.
I am an Associate Professor at The Rockefeller University in New York.
For those of you who may not know, Rockefeller is located in one of the best and
safest areas of New York City. With neighboring Cornell Medical School and
Sloan-Kettering Cancer Center, this is one of the most scientifically exciting
communities to work in. Subsidized housing is available across the street, and
child care is available on campus.
My laboratory is studying endothelial adhesion molecules and their role
in inflammation. We are taking a multidisciplinary approach using techniques
of cell biology, immunology, molecular biology, and biochemistry. We have
identified and cloned two unique adhesion molecules concentrated in the inter-
cellular borders between endothelial cells. These are PECAM-1 (CD31) and the
endothelial-specific cadherin, cad5 (VE-cadherin). Our lab has shown that PECAM
is required for the migration of leukocytes through the endothelial junctions
during inflammation. The project involves determining the ultrastructural
distribution of these proteins along the endothelial junctions in vitro and
in vivo. (We have also cloned the corresponding mouse PECAM and cad5.) We
believe that part of the function of these molecules is determined by their
distribution along the membrane.
This would be an excellent oppportunity for someone trained in electron
microscopy to further his/her skills as well as to learn new techniques of
molecular biology and immunology. Our group interacts well and often, so that
everyone can benefit from the others' knowledge and skills.

Interested parties should send a letter along with a C.V. to the address
below. For specific questions or more details, please fax (212) 327-8875. I
look forward to hearing from you.

Sincerely,

William A. Muller, MD, PhD
Laboratory of Cellular Physiology and Immunology
Box 176
The Rockefeller University
11230 York Avenue
New York, NY 10021-6399


P.S. Thanks to Chris Jeffries and Jeanne Barker for helping me connect to this
listserver.




From: PRING :      richard.pring-at-bbsrc.ac.uk
Date: Tue, 28 Mar 1995 04:19:50 -0600
Subject: embedding plant material

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I have problems in embedding two types of material, one is developing oil seed
rape pods in order to look at changes leading to pod shatter. Even after
several days in 100% Spurr resin, the centre of the section, which often
comtains the developing seed, remains soft and falls out when sectioning.
Similarly, in a study on the infection of rubber leaves by colletotricum spp.,
when sectioning infected material the sections split along the leaf surface.
Any ideas on overcoming these problems? I routinely use Spurr hard resin and go
through propylene oxide after ethanol dehydration. I have also tried holding
the specimens at 45C in the moulds for 24h before raising the temperature to
65C for full polymerisation. I have tried LRWhite with no improvement

Richard pring-at-lars.bbsrc.ac.uk




From: Jan Coetzee EM Univ Pretoria :      JANC-at-ccnet.up.ac.za
Date: Tue, 28 Mar 1995 14:51:03 GMT+2
Subject: Re: embedding plant material

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Richard Pring mentioned:
} I have problems in embedding two types of material, one is developing oil
} seed rape pods in order to look at changes leading to pod shatter. Even
} after several days in 100% Spurr resin, the centre of the section, which
} often comtains the developing seed, remains soft and falls out when
} sectioning. Similarly, in a study on the infection of rubber leaves by
} colletotricum spp., when sectioning infected material the sections split
} along the leaf surface. Any ideas on overcoming these problems? I routinely
} use Spurr hard resin and go through propylene oxide after ethanol
} dehydration. I have also tried holding the specimens at 45C in the moulds
} for 24h before raising the temperature to 65C for full polymerisation. I
} have tried LRWhite with no improvement

Very often the failure to infiltrate material adequately with resin is
due to inadequate fixation and not to inadequate resin infiltration.
Especially when processing oily samples it is necessary to fix {very} well to
destroy all membrane semi-permeability and to stabilise the lipids. Going
to extremes during the fixation may lead to substandard structural
preservation, but may be the only way to adequately infiltrate the sample.
One such overkill schedule is: Fix material 24h at 40deg C in 2.5%
glutaraldehyde, then postfix for another 24h in several changes of
osmium, also at 40deg C (take due care during this step). Dehydrate in
acetone, use a few prop. oxide rinses and embed in your standard resin.
Other methods (adding Malachite Green to aldehydes in DMSO) are also known
and may very well work.
All these methods have in common that they are not optimal as far as
structural preservation is concerned, but sometimes they may be the only ones
that will result in thin sections.
Richard's second problem, that of the adhesion of the resin to the leaf
surface is a result of the wax layer on the leaf surface forming a parting
layer between the leaf and the resin. One solution is to use an epoxy
formulation that is not as easily parted from cuticular waxes - Quetol may be
a possibility.











Jan Coetzee
Unit for Electron Microscopy Tel:+27-12-420-2075
University of Pretoria Fax:+27-12-342-1738
Pretoria Internet:janc-at-ccnet.up.ac.za
0002 South Africa




From: USRND::U096585 U096585 27-MAR-1995 10:15
Date: Tue, 28 Mar 1995 08:20:09 -0500
Subject: Re: Upgrading old EDS systems

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Greetings Kristof...

I have a an old Kevex EDS system hooked up to an "old" Cameca MBX
microprobe....both about 15 years old. I've recently replaced the multi-
channel analyzer with a 4pi processing system and a Mac computer. The 4
pi system costs around $6,000 and is easy to incorporate into many
different EDS systems.

The 4 pi is relatively cheap, it's efficient and easy to operate and is
capable of interfacing with many different EDS systems. Not only can it
generate very nice EDS spectra through DTSA, it does an excellent job
imaging elementally specific X-ray transitions. The quality of these
EDS maps is close to that of the digital WDS maps that we generate on
our SX-50 microprobe. It's really quite impressive.

If you are interested, I would be happy to FAX you some information on
the 4 pi system as well as some EDS images we have generated using that
system. Just let me know....

or here's 4 pi info:

4 Pi Analysis, Inc.
3500 Westgate Dr., Suite 403
Durham, NC 27707-2534
Tel: 919-489-1757
FAX: 919-489-1487

e-mail
go4pi-at-applelink.apple.com

Good Luck and best regards

Cary Black
Microscopist
The Dow Chemical Co.

ckblack-at-dow.com
517-636-5760




From: Giles John E Jr :      giles_john_e_jr-at-space.honeywell.com
Date: 28 Mar 1995 09:05:57 U
Subject: SEM: Thermal Printers

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Has anyone had experience with long term storage of images from a thermal
printer (Codonics, Seikosha, etc.)? I have noticed that the images start to
fade away after exposure (months) to fluorescent lights. I have been told that
this will happen even when they are protected from exposure to light. Any
suggestions for storage or other experience would be appreciated.

John Giles
Honeywell Space Systems
(813) 539-2270
(813) 539-3630 Fax
e-mail: jegiles-at-space.honeywell.com




From: OSRAM Sylvania :      osiit9-at-epix.net
Date: Tue, 28 Mar 1995 18:14:05 +0000 (GMT)
Subject: Re:Thermal print storage problems

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Message-Id: {199503281401.PAA03757-at-gate.sbbio.be}

John,

We've had a Seikosha thermal printer for 4 years and still have some of
the first images printed with it saved. The images have faded slightly
from gray to brown, but you can only tell if you hold next to a new
print. The best storage for them is in a binder or folder that is stored
in a closet or file cabinet. As long as they aren't exposed to drastic
variations in heat they should store for long periods of time with
minimal fading.


Gail Meyers
OSRAM SYLVANIA INC
(717) 268-5340




From: Heinz Hemken :      hhemken-at-cell.cinvestav.mx
Date: Tue, 28 Mar 1995 13:36:38 -0800 (PST)
Subject: Re: Upgrading old EDS systems

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Just out of curiosity, what is an EDS system?

------------------------------------------------------------------
Heinz Hemken
Departamento de Biologia Celular, CINVESTAV-IPN
http://cell.cinvestav.mx/bchh.html





From: gkrichau-at-unlinfo.unl.edu (Gary Krichau)
Date: Tue, 28 Mar 1995 15:19:18 -0500
Subject: Subscribe microscopy

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Subscribe microscopy Gary Krichau

Gary Krichau

Central Facility for Electron Microscopy
University of Nebraska-Lincoln
___________________________________________





From: JOHNA-at-SCI.WFEB.EDU
Date: Tue, 28 Mar 1995 09:49:06 -0400 (EDT)
Subject: PLP fix

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Dear Michel,

PLP fix recipe can be found: Nakane, Paul K., 1975. Recent progress in the
peroxidase-labeled antibody method. Ann. N.Y. Acad. Sci. 254:203-211.

There also is an abstract in the Abstacts of thr 13th Annual Meeting of
ASCB by McLean and Nakane - # 418 on page 209a.

It's a good fixative for the preservation of both antigenicity and
ultrastructure.

Good Luck,

JOHNA

___________________________________________________
| |
| John G. Aghajanian, Ph.D. |
| Worcester Foundation for Experimental Biology |
| 222 Maple Avenue |
| Shrewsbury, MA 01545-2737 |
| |
| Tel: 508 842-8921 ext. 147, 161 |
| Fax: 598 842-9632 |
| JOHNA-at-sci.wfeb.edu |
| |
|_________________________________________________|





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Tue, 28 Mar 1995 17:03:18 -0600 (CST)
Subject: EDS is.....

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EDS is the abbreviation for

Energy Dispersive Spectroscopy, which is probably more
correctly called X-ray Energy Dispersive Spectroscopy (XEDS)
but various other rearrangements of the letters exist in
the literature (EDXS,...)

It is an analytical methodology in which a solid state semi-conducting
detector (either Si or Ge based) is used to measure the energy distribution
of X-rays emitted from a specimen. The X-ray's may be excited by
some sort of probe (usually a focussed beam of electrons, ions,
or photons). The "Spectrum" is then displayed on a graphical output
device (read computer screen) and the relative intensity of
the measured characteristic X-ray peaks, analyzed to determine
the relative elemental composition of the material.

Any good text book on Scanning Electron Microscopy or Electron
Probe Microanalysis (published within the last 10 years or so
will have a chapter or two on the subject).



Nestor....
Your Friendly Neighborhood SysOp.






From: xxie-at-lsuvm.sncc.lsu.edu (Xiaogang Xie)
Date: Tue, 28 Mar 1995 18:34:22 -0600
Subject: Re: Upgrading old EDS systems

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An article about 4pi system for EDS was published in SCANNING Vol.
14, 233-240 (1992). The title is:

Mac X-ray: The NIST 'DTSA' program and the 4pi analysis board.

one of the authors is:
David C. Joy
EM lab
U. of Tennessee
Knoxville, TN 37996-0810

Our lab also installed both the 4pi spectral engine boards and the
4pi scanning interface box. We have been enjoying NIH Image and several
other programs in playing the SEI, BEI, and X-ray mapping.

Xiaogang

****************************************
* Xiaogang Xie *
* Department of Geology and Geophysics *
* Louisiana State University *
* Baton Rouge, LA 70803 *
* Office (504)388-2240 *
* Fax (504)388-2302 *
****************************************






From: sfzane-at-unccvm.uncc.edu (Sandra F. Zane)
Date: Tue, 28 Mar 1995 11:51:28 -0500
Subject: curing resin summary

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As promised, following is the summary of responses received from my
question, "How many of you have your curing ovens vented to a hood?".

From a total of 11 responses, one containing knowledge of two others,
there were only two who were not curing their resins in, or venting their
ovens to a hood. Both are making provisions to do so. Nine actually place
their ovens in the hood while two are vented to the hood.
There was only one reference sited...that being an article in the
Proceedings of the RMS Vol. 16, pp. 265-270, 1981.
Most of us seem to subscribe to the "just to be sure" school of reason.
I attempted to thank each of you who responded individually, but I
think that I may have lost one of you. So let me say now....thank you very
much.

Sandra Zane
Sandra F. Zane, EM Tech.
Biol. Dept. UNCC
Charlotte, NC 28223
sfzane-at-unccvm.uncc.edu
Fax (704) 547-3128





From: A. Kent Christensen :      akc-at-umich.edu
Date: Tue, 28 Mar 1995 10:19:46 -0500 (EST)
Subject: Re: TEM: PLP fixative reference/recipe

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The original paper on PLP was McLean and Nakane 1974, J Histochem
Cytochem 22:1077-1083. Also see Pino 1984, Stain Technol. 59:307.

Some of the most impressive uses of PLP came out of Marilyn
Farquhar's lab. If I remember correctly the ICC that Farquhar/Brown did
utilized PLP, and would be a good source of method. For example, see:
Cell 36:295(1984). PNAS 81:5135 (1984). JCB 106:1863 (1988). Meth Cell
Biol 31:553 (1989).

Good luck.

A. Kent Christensen
Department of Anatomy and Cell Biology
University of Michigan Medical School
Ann Arbor, MI 48109-0616
{akc-at-umich.edu}

---------------------------------

On Mon, 27 Mar 1995, Michel Deschuyteneer wrote:

} I would greatly appreciate the reference and/or the recipe for the
} Paraformaldehyde - Lysine - Periodate (PLP) fixative.
} Thank you very much in advance.
}
} Regards,
} Michel.
} Michel Deschuyteneer deschuyt-at-sbbio.be
} Scientist - Electron Microscopy Laboratory
} SmithKline Beecham Biologicals
} Rue de l'Institut, 89 - B1330 Rixensart, BELGIUM
} Tel: +32-2-656 9290 Fax: +32-2-6568113
}
}
}
}




From: Chris Varga :      chris-at-lis.rch.unimelb.edu.au
Date: Wed, 29 Mar 1995 13:37:35 +1000
Subject: Re: TEM: PLP fixative reference/recipe

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Please unsubscribe!





From: Dr. Molnar Peter :      MOLNARP-at-lib.dote.hu
Date: Wed, 29 Mar 1995 11:26:06 GMT+0100
Subject: subscription request

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From: Richards, P, Peter :      RETEP-at-anat.uct.ac.za
Date: Wed, 29 Mar 1995 14:09:14 SAST-2
Subject: Re:Diamond Knives

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Message-Id: {m0rttW7-0009hbC-at-uctmail2.uct.ac.za}

To Everyone out there:

Just doing a quick survey as to the price of Diamond microtomy knives.

Could anyone who has bought one recently tell me what the
going cost is for a diamond 5mm ultramicrotyomy knife is, in $ or
pounds sterling?

Thanks in advance

Peter
_______________________________________________________________


-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
-at- -at-
-at- Peter D. G. Richards -at-
-at- Dept Anatomy and Cell Biology -at-
-at- UCT Medical School -at-
-at- Observatory -at-
-at- 7925 -at-
-at- RSA -at-
-at- Tel: 021-406 6285. -at-
-at- Internet: retep-at-anat.uct.ac.za -at-
-at- -at-
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-




From: ckblack-at-dow.com (U096585)
Date: Wed, 29 Mar 1995 08:21:17 -0500
Subject: Re: EDS system upgrades

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Greetings Kristof...

I have a an old Kevex EDS system hooked up to an "old" Cameca MBX
microprobe....both about 15 years old. I've recently replaced the multi-
channel analyzer with a 4pi processing system and a Mac computer. The 4
pi system costs around $6,000 and is easy to incorporate into many
different EDS systems.

The 4 pi is relatively cheap, it's efficient and easy to operate and is
capable of interfacing with many different EDS systems. Not only can it
generate very nice EDS spectra through DTSA, it does an excellent job
imaging elementally specific X-ray transitions. The quality of these
EDS maps is close to that of the digital WDS maps that we generate on
our SX-50 microprobe. It's really quite impressive.

If you are interested, I would be happy to FAX you some information on
the 4 pi system as well as some EDS images we have generated using that
system. Just let me know....

or here's 4 pi info:

4 Pi Analysis, Inc.
3500 Westgate Dr., Suite 403
Durham, NC 27707-2534
Tel: 919-489-1757
FAX: 919-489-1487

e-mail
go4pi-at-applelink.apple.com

Good Luck and best regards

Cary Black
Microscopist
The Dow Chemical Co.

ckblack-at-dow.com
517-636-5760





From: u096585-at-mdanl3.md.dow.com (U096585)
Date: Wed, 29 Mar 1995 08:21:17 -0500
Subject: Re: EDS system upgrades

Contents Retrieved from Microscopy Listserver Archives
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Greetings Kristof...

I have a an old Kevex EDS system hooked up to an "old" Cameca MBX
microprobe....both about 15 years old. I've recently replaced the multi-
channel analyzer with a 4pi processing system and a Mac computer. The 4
pi system costs around $6,000 and is easy to incorporate into many
different EDS systems.

The 4 pi is relatively cheap, it's efficient and easy to operate and is
capable of interfacing with many different EDS systems. Not only can it
generate very nice EDS spectra through DTSA, it does an excellent job
imaging elementally specific X-ray transitions. The quality of these
EDS maps is close to that of the digital WDS maps that we generate on
our SX-50 microprobe. It's really quite impressive.

If you are interested, I would be happy to FAX you some information on
the 4 pi system as well as some EDS images we have generated using that
system. Just let me know....

or here's 4 pi info:

4 Pi Analysis, Inc.
3500 Westgate Dr., Suite 403
Durham, NC 27707-2534
Tel: 919-489-1757
FAX: 919-489-1487

e-mail
go4pi-at-applelink.apple.com

Good Luck and best regards

Cary Black
Microscopist
The Dow Chemical Co.

ckblack-at-dow.com
517-636-5760

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From: Leonard Radzilowski :      lrg8037-at-uxa.cso.uiuc.edu
Date: Wed, 29 Mar 1995 09:50:22 -0600 (CST)
Subject: unsubscribe

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From: Heinz Hemken :      hhemken-at-cell.cinvestav.mx
Date: Wed, 29 Mar 1995 09:06:43 -0800 (PST)
Subject: Re: EDS system upgrades

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Thanks to all who have explained to me what EDS is. I got several
enlightening and courteous replies. This is a great list and a great
community!

Keep up the rich info stream!

------------------------------------------------------------------
Heinz Hemken
Departamento de Biologia Celular, CINVESTAV-IPN
http://cell.cinvestav.mx/bchh.html





From: bdgranby-at-student.wisc.edu (ben granby)
Date: Wed, 29 Mar 1995 12:01:02 -0500
Subject: userlist

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Please add me to uour mailing list. Thank You.





From: bdgranby-at-student.wisc.edu (benjamin granby)
Date: Wed, 29 Mar 1995 12:01:16 -0500
Subject: userlist

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Please add me to your mailing list. Thank You.





From: bdgranby-at-student.wisc.edu (Ben Granby)
Date: Wed, 29 Mar 1995 12:02:47 -0500
Subject: Userlist

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From: bdgranby-at-students.wisc.edu (Benjamin Dov Granby)
Date: Wed, 29 Mar 1995 12:09:49 -0500
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Date: Wed, 29 Mar 1995 15:08:55 -0500
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From: Jay Jerome :      jjerome-at-isnet.is.wfu.edu
Date: Wed, 29 Mar 1995 14:55:06 -0500 (EST)
Subject: SEM Short Courses for Materials Scientist

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This request is for a colleague:

He is using SEM for industrial applications (various analyses of Clay,
paints, paper, etc). They are looking for a good short course on SEM in
materials science. The dates of both the Lehigh and McCrone courses will
not fit into their schedule. I know of a course at Univ Michigan which I
told them about. Are there any others?-
Thanks for any help you can render.


Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-isnet.is.wfu.edu *
**************************************************************





From: Lalonde Sylvie :      lalonds-at-ERE.UMontreal.CA
Date: Wed, 29 Mar 1995 11:47:52 -0500 (EST)
Subject: LM.methacrylate resin.removal and UV polymerization

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Hi folks,

I am trying to do in situ hybridization and immunohistochemistry on
glycol methacrylate-embedded wheat pollen. The reason I am using that
resin, despite its uglyness to work with, is that the infiltration of
pollen is very good and the overall morphology seems to be well
preserved. But to gain a better access to the target in the tissue I
would like to remove the resin after sectionning. The resin was
polymerized with benzoyl peroxide at 55C. Is there a way to remove it
without affecting antigenicity of the tissue???

If I cannot remove the glycolmethacrylate, I am planning to use a mixture
of butyl and methyl methacrylate with a polymerization under UV.
According to litterature, I can remove it with acetone. Some people use
benzoin, others use benzoin ethyl ether; what is the difference between
the two? Can I use bezoyl peroxide instead? I know that UV polymerization
can lead to inconsistancy in the polymerization, is the use of a 4W placed
on top of the specimen at a distance of about 10 cm could be good?

Since I do not subscribe to this group could you send me directly any
suggestions you may have.

Thanks in advance

Sylvie Lalonde
lalonds-at-ere.montreal.ca







From: Dwight Beebe :      beebed-at-ERE.UMontreal.CA
Date: Wed, 29 Mar 1995 12:20:44 -0500 (EST)
Subject: LM: fluorescent probes for ER

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Greetings:
I'm interested in using fluorescent probes to clarify the
relationship between certain cellular structures, specifically the ER and
what I believe to be vacuoles. Some of my colleages have suggested that
the vesicles I believe to be vacuolar in origin may be dilated ER. In
looking for likely probes I've found refences in the Molecular Probes
handbook to DiIC (a long-chain carbocyanine) and Rhodamine B as
ER-specific dyes. However, the cited references are based on experiments
on animals. Ideally, I would like to use two probes that will
discriminate between ER and vacuoles. Does anyone have experience with
these dyes or could someone point me towards some references? I'm
working with leaf tissue, specifically cells with the minor veins.
Many thanks in advance.

Dwight Beebe
IRBV, Dept. de sciences biologiques beebed-at-ere.umontreal.ca
Universite de Montreal Voice:514-872-4563
4101, rue Sherbrooke est FAX:514-872-9406
Montreal, PQ H1X 2B2 Canada








From: almonte-at-medcolpa.edu
Date: Wed, 29 Mar 1995 13:11:13 -0400
Subject: LM: fluorescent probes for ER

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Dear friends,
Thanks for your responses. As you all may remember I was having
some crossing over problem between FITC and Rhodamine-that's what I
thought... My problem seems to be autoflorescense, yeah that simple and
basic, I just overlooked it.
I just looked at the neurons with just plain solution, they
flouresce beautifully under the rhodamine filter, and slightly under the
FITC filter and no fluorescence under the Cy5 filter. Also, I had a control
(no antibodies present) which I fixed with 4% paraformaldehyde and used 3%
normal goat serum as blocking reagent. This control flouresce better under
the FITC filter than under the Rhodamine filter, the opposite of the
control without fixative. It does not flouresce under the Cy5 filter.
Also, I did a control with CY5 and Cy3. Again the cells flouresces
under the FITC and rhodamine Filters but not under the CY5 filter.
So my solution seems to stay away from FITC, and rhodamine. Any
suggestion on how I can better solve my autoflourescence problem will be
appreciated. Thanks again for your responses.
Your friend,
Ciprian

__________________________________________________________
Ciprian Almonte
Medical College of Pennsylvania
Dept. of Anatomy and Neurobiology E-mail: almonte-at-medcolpa.edu
3200 Henry Ave. Voice: (215) 842-4081
Philadelphia, PA 19129 Fax: (215) 843-9082
__________________________________________________________







From: Eric Wang :      ewang-at-u.washington.edu
Date: Wed, 29 Mar 1995 17:27:21 -0800 (PST)
Subject: Re: SEM Short Courses for Materials Scientist

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X-Sender: ewang-at-mead2.u.washington.edu


There is a very good SEM short course given by Botany department of
university of washington in Seattle. This course covers various subject
and the scheduel is flexable. The instructor of the course is Barbra Reine.

Tel # for Botany Department: (206) 543-1942




On Wed, 29 Mar 1995, Jay Jerome wrote:

} This request is for a colleague:
}
} He is using SEM for industrial applications (various analyses of Clay,
} paints, paper, etc). They are looking for a good short course on SEM in
} materials science. The dates of both the Lehigh and McCrone courses will
} not fit into their schedule. I know of a course at Univ Michigan which I
} told them about. Are there any others?-
} Thanks for any help you can render.
}
}
} Jay Jerome
} **************************************************************
} * aka: W. Gray Jerome *
} * Dept. of Pathology *
} * Bowman Gray School of Medicine of Wake Forest University *
} * Medical Center Blvd *
} * Winston-Salem, NC 27157-1092 *
} * 910-716-4972 *
} * jjerome-at-isnet.is.wfu.edu *
} **************************************************************
}
}




From: Kittel Agnes :      kittel-at-kokiux.koki.hu
Date: Thu, 30 Mar 1995 09:30:06 +0200 (MET DST)
Subject: subscription reguest and a guestion

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If someone knows, please, write me, which type of NCAM antibody can work
for post- embedding method? (Araldite 6005 or Epon resin is used and pre
embedding I have made an enzyme histochemical reaction. Fixation :
paraformaldehyde- glutaraldehyde in cacodylate buffer)
Thanks!
Agnes Kittel






From: H2993Pec-at-huella.bitnet
Date: 30 Mar 95 09:56:46 +0100
Subject: Subscription request

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Subscription request





From: Tina Carvalho :      tina-at-halia.pbrc.Hawaii.Edu
Date: Wed, 29 Mar 1995 22:27:56 -1000 (HST)
Subject: C evaporation for EDS

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Both my lab and another lab here in Hawaii have Denton DV-502 vacuum
evaporators. My evaporator is perfectly fine for my needs; I can
carbon coat Formvar grids or prepare pure carbon substrates for TEM.
The other lab, however, wants to carbon coat for SEM, with and without
EDS. Their problem is that they cannot get the carbon to evaorate long
enough to get a thick enough coating, and neither can I on my
instrument. We both have the same spring-steel (?) driven carbon rod
holders (which differ from the gravity-fed system in the very old Denton
in still another Hawaii lab and also from the coiled spring feed on the
non-adjustable holders). The problem seems to be that there is
enough resistance somewhere that everything heats up like crazy, the
moveable parts expand enough to get stuck, and the whole feed process
stops until the unit cools down or blows a fuse, whichever comes first.
This has happened with old holders, brand-new ones, and everything else
I have been able to devise. Has anyone else had and oversome this
problem? Any hints and tips will be appreciated!

Mahalo and aloha,
Tina Weatherby Carvalho
Biological EM Facility
University of Hawaii





From: ddd-at-techunix.technion.ac.il (dd)
Date: Thu, 30 Mar 1995 12:53:55 +0200
Subject: Light scrambler for HBO lamp

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We need information on where to get a light scrambler for homogeneous
illumination -to couple an HBO light source to a Zeiss microscope for
fluorescence work. Sources, FAX numbers, price ranges -any information
would be highly appreciated.
dd




Daniel Dagan,
Dept. Biophysics and Physiology,
Faculty of Medicine, Technion,
Israel Institute of Technology,
POBox 9697
Haifa 31096
ISRAEL

Tel: 972-4-295566
Fax: 972-4-533183
e-mail: ddd-at-techunix.technion.ac.il






From: A. Kent Christensen :      akc-at-umich.edu
Date: Thu, 30 Mar 1995 08:05:54 -0500 (EST)
Subject: Autofluorescence problems

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From your description, it sounds like you need quenching rather than
more blocking. Blocking uses miscellaneous protein to block non-specific
protein-binding sites in the tissue, but doesn't actually deal with
autofluorescence. Quenching uses reducing agents such as ammonium
chloride or sodium borohydride to reduce double bonds in the tissue, which
are usually the main source of autofluorescence. Try the following: make
up a fresh 1% solution of sodium borohydride in distilled water (careful,
borohydride is toxic), and leave a few drops of it on your sections for
about 10 minutes early in your immunocytochemical run (before the primary
antibody step). This should reduce the inherent autofluorescence in your
tissue.

A. Kent Christensen, Department of Anatomy and
Cell Biology, University of Michigan Medical School, {akc-at-umich.edu}

------------------------------------

On Wed, 29 Mar 1995 almonte-at-medcolpa.edu wrote:

} Dear friends,
} Thanks for your responses. As you all may remember I was having
} some crossing over problem between FITC and Rhodamine-that's what I
} thought... My problem seems to be autoflorescense, yeah that simple and
} basic, I just overlooked it.
} I just looked at the neurons with just plain solution, they
} flouresce beautifully under the rhodamine filter, and slightly under the
} FITC filter and no fluorescence under the Cy5 filter. Also, I had a control
} (no antibodies present) which I fixed with 4% paraformaldehyde and used 3%
} normal goat serum as blocking reagent. This control flouresce better under
} the FITC filter than under the Rhodamine filter, the opposite of the
} control without fixative. It does not flouresce under the Cy5 filter.
} Also, I did a control with CY5 and Cy3. Again the cells flouresces
} under the FITC and rhodamine Filters but not under the CY5 filter.
} So my solution seems to stay away from FITC, and rhodamine. Any
} suggestion on how I can better solve my autoflourescence problem will be
} appreciated. Thanks again for your responses.
} Your friend,
} Ciprian
}
} __________________________________________________________
} Ciprian Almonte
} Medical College of Pennsylvania
} Dept. of Anatomy and Neurobiology E-mail: almonte-at-medcolpa.edu
} 3200 Henry Ave. Voice: (215) 842-4081
} Philadelphia, PA 19129 Fax: (215) 843-9082
} __________________________________________________________




From: Richard Lee :      richard_lee-at-qmgate.anl.gov
Date: 30 Mar 1995 11:38:54 -0600
Subject: Formation of geodes

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11:35 AM 3/30/95

Does anyone know the mechanism of geode crystal formation in the midwest? And
why do some geodes contain oil?

I have an Idea!




From: BILL RHOTEN :      RHOTEN-at-musom01.mu.wvnet.edu
Date: Thu, 30 Mar 1995 13:31:05 +1100
Subject: test mess, as in test message

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From: xxie-at-lsuvm.sncc.lsu.edu (Xiaogang Xie)
Date: Thu, 30 Mar 1995 12:48:35 -0600
Subject: an introductary paper about 4pi system

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A message about an article regarding the 4pi system sent out couple of
days ago was bounced back. The paper was published in SCANNING Vol. 14,
233-240 (1992). The title is:

Mac X-ray: The NIST 'DTSA' program and the 4pi analysis board.

One of the authors is:
David C. Joy
EM lab
U. of Tennessee
Knoxville, TN 37996-0810

Our lab has installed both the 4pi spectral engine boards and the
4pi scanning interface unit. We have also been enjoying NIH Image and
several other programs in playing the SEI, BEI, and X-ray mapping.

Xiaogang

****************************************
* Xiaogang Xie *
* Department of Geology and Geophysics *
* Louisiana State University *
* Baton Rouge, LA 70803 *
* Office (504)388-2240 *
* Fax (504)388-2302 *
****************************************






From: Self :      MUSOM01/RHOTEN
Date: Thu, 30 Mar 1995 13:31:05
Subject: test mess, as in test message

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------- Forwarded Message Follows -------

How was the test mess?Microscopy BBS




From: Dennis Shubitowski :      dennis%odin-at-odin.morph.med.umich.edu
Date: Thu, 30 Mar 1995 14:51:44 -0500 (EST)
Subject: Re: Formation of geodes

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Errors-To: {dennis-at-odin.morph.med.umich.edu}



On 30 Mar 1995, Richard Lee wrote:

} Does anyone know the mechanism of geode crystal formation in the midwest? And
} why do some geodes contain oil?

According our resident geologist, geodes form when percolating waters
precipitate into hollow cavities. Whatever happens to be carried with the
water is deposited in layers into the cavity (this includes oil).


Dennis








From: Joyce Craig :      bafpjec-at-uxa.ecn.bgu.edu
Date: Thu, 30 Mar 1995 14:51:31 -0600 (CST)
Subject: liposomes

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Does anyone have a protocol for visualization of liposomes?
I would like to be able to thin section for TEM. Should anything be seen
at the LM level?

thanks,
Joyce Craig




From: xxie-at-lsuvm.sncc.lsu.edu (Xiaogang Xie)
Date: Thu, 30 Mar 1995 12:06:43 -0600
Subject: advises about filament needed

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Advises about W filament needed:

1. Has anyone made comparison about the performance, life time, and
potential contamination between new filament and rebuilt one. The prices
are four times difference.
2. Which company supplies better batch of filaments. This information may
mail to me directly if you feel it may offend some one.
3. Last year, we had a batch of new filaments that tend to form thin film
of metal sticking on the tip of the filament, though the heating
temperature was low (with slightly large distance between filament and the
cap, and the heating current is limited by a stopper). Is this a problem
due to impurity of filament? Any idea?

Thanks for any input.

Xiaogang

****************************************
* Xiaogang Xie *
* Department of Geology and Geophysics *
* Louisiana State University *
* Baton Rouge, LA 70803 *
* Office (504)388-2240 *
* Fax (504)388-2302 *
****************************************






From: xxie-at-lsuvm.sncc.lsu.edu (Xiaogang Xie)
Date: Thu, 30 Mar 1995 12:44:57 -0600
Subject: Re: C evaporation for EDS

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Tina:
In our lab, the DV-502 is used for C coating SEM and Microprobe
samples for EDS and WDS works.

1. The length and size of the reduced C rod are important factors for the
coating thickness. The coarser C rod does not only supply more C but also
support longer reduced C rod. However, coarser C rod needs higher current
to reach the evaporation temperature, hence it might hit some limits of the
machine, like burn the fuses. This has to be balanced.
2. You also need to properly control the heating current during
evaporating. Specially, you need quickly bring the current back to some
position where evaporation can last longer at the very beginning when the
evaporating starts.

Xiaogang

****************************************
* Xiaogang Xie *
* Department of Geology and Geophysics *
* Louisiana State University *
* Baton Rouge, LA 70803 *
* Office (504)388-2240 *
* Fax (504)388-2302 *
****************************************






From: kayton-at-ohsu.edu (Robert Kayton,MAC,CROET)
Date: Thu Mar 30 17:12:16 PST 1995
Subject: TEM: Gold particle counting

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Message-Id: {m0ruVG4-0007AdC-at-stjohns.ohsu.edu}
Message-Version: 2
} To: microscopy-at-aaem.amc.anl.gov

I have colleague that needs help setting up an automatic computer based method
to count gold particles on TEM micrographs. He has NIH image, a good
Macintosh and can import digital images but is having difficulties getting
accurate counts of the gold particles. If anyone has experience with this
please let me know.

Bob Kayton
kayton-at-ohsu.edu




From: Doug Davis :      doug_davis-at-maillink.berkeley.edu
Date: 30 Mar 1995 16:28:16 -0800
Subject: Re: C evap for EDS

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Message-ID: {n1415541637.55689-at-maillink.berkeley.edu}
"Tina Carvalho" {tina-at-ahi.pbrc.hawaii.edu}
X-Mailer: Mail*Link SMTP-QM 3.0.2

Subject: Time: 4:13 PM
OFFICE MEMO RE} C evap for EDS Date: 3/30/95

Tina- check your ceramic insulators through which the rod holder slides.
If the ceramic gets enough carbon or metal on it, it creates a conductive
pathway which corrupts your normal electrical flow and sends current through
the metal parts of the electrode instead of the carbon rod. Could explain the
hot metal and blown fuses from over-amping the system. This can even melt
some of the smaller metal parts.
Cleaning the ceramic is tough because of the porous surface and chances are
the ceramic will crumble from the excessive heating when you dissassble the
holder. Suggest you order some new ones from Denton and keep some spares.
Have you tried the carbon yarn yet? Works well, never sparks or arcs,
heavier thicknesses can be produced by reducing the source to target distance.
Last time I checked, Denton had the best price. -Doug





From: VanderWood-at-aol.com
Date: Fri, 31 Mar 1995 01:37:10 -0500
Subject: Re:C evap for EDS

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We have done a comparison study of rods from different sources, and found
some to be unworkable for SEM/EDS coating in our 502. Rather than belittle
the ones that don't work, I can recomment those from Ladd. Hope this helps.

Tim VanderWood
MVA, Inc.




From: Doug Davis :      doug_davis-at-maillink.berkeley.edu
Date: 30 Mar 1995 09:49:11 -0800
Subject: Denton evap

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Message-ID: {n1415565559.16197-at-maillink.berkeley.edu}
"Tina Carvalho" {tina-at-ahi.pbrc.hawaii.edu}
X-Mailer: Mail*Link SMTP-QM 3.0.2

Subject: Time: 9:28 AM
OFFICE MEMO Denton evap Date: 3/30/95

Tina- We have a 502A with turbo. One thing to check immediatly is the
ceramic insulators which the electrode holder slides through. In the past,
repeated applications of heavy coatings will eventually create a conductive
surface coating on these parts and current through the carbon rod will be
reduced as it flows along this alternate pathway. In extreme cases, carbon
will not evaporate and metal parts of the electrode will actually begin to
melt. Good thing your fuses are blowing rather than your top. The insulators
are tough to clean because of the porous surface and you may find that they
crumble to dust when you remove them due to the heating they have been
subjected to. It's good to have a few extras around as this is a chronic
problem.
Have you tried the carbon yarn yet in the Denton? It is impossible to
blowout/arc as with the rods and is much more controllable, just evaporate
until it burns through. Heavier coatings can be acquired by reducing the
distance from the source. Look out for metal contamination of the yarn if you
evaporate shadowing metals; it shows up as tiny electron-dense spots on you
substrates. Use the shutter to protect the unused yarn on the bobbin. You
can get the yarn directly from Denton for the best price; e-mail me if you
need info. -Doug





From: Laurie Frederick :      frederick_laurie-at-vanlab.paprican.ca
Date: Thu, 30 Mar 1995 10:44:55 PDT
Subject: SEM: Carbon Evaporators

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Message-Id: {MAILQUEUE-101.950330104455.320-at-vanlab.paprican.ca}
To: microscopy-at-aaem.amc.anl.gov

Hello,
We are intending to purchase a carbon evaporation unit for coating
SEM/EDX samples . We have received quotes for 6 systems but have
narrowed the choises to the following on the basis of cost:
Denton 502A
Ladd
Edwards 306
The price range for these units is $24,000-$35,000 in Canadian dollars.
We are very tight for money (of course) but do not want to buy a unit
which will give us continuous headaches with overheating, jamming of
the carbon rod feed mechanism or having to evacuate several times in
order to recoat because enough carbon could not be applied the first
time.
If you have experience with any of these units, either positive or
negative, I would appreciate hearing your comments. If you have any
experience with using 1/4" rods vs. 1/8" rods or "reduced section"
versus conically tipped rods, please pass it along.

I was all set to make a purchase before I read the recent comments on
the listserver.
Thanks very much for any assistance.
Laurie


______________________________________________________________________
Laurie Frederick, A.SC.T. PAPRICAN
Corrosion Control Group 3800 Wesbrook Mall
The Pulp and Paper Research Vancouver, B.C.
Institute of Canada Canada V6S 2L9

Email: frederick_laurie-at-vanlab.paprican.ca
Tel: 604-222-3200 Fax: 604-222-3207




From: Laurie Frederick :      frederick_laurie-at-vanlab.paprican.ca
Date: Thu, 30 Mar 1995 14:35:52 PDT
Subject: EDX Contacts

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Message-Id: {MAILQUEUE-101.950330143551.320-at-vanlab.paprican.ca}
To: microscopy-at-aaem.amc.anl.gov

Hi,
I am trying to wrap up a summary of referances on EDX and require
some information:
1) Has the book from MAS entitled "X-ray Spectrometry in Electron
Beam Instruments" by Dave Williams and Dale Newbury actually been
published yet and if so, is there a phone number for orders?

2) Is anyone familiar with an EDX upgrade system from "Aptec"? I
believe it is located somewhere in Ontario, Canada but I am not sure if
the name I have is a company or product name. Contact # ?

3) When and where is the next International Congress on Electron
Microscopy (ICEM) and what is a contact number for information?

4) When and where is the next International Conference on X-ray
Optics and Microanalysis (ICXOM) and what is a contact # for info?

I will be making a summary of the information I have gathered on EDX
available shortly. Thanks for any assistance.
Laurie

______________________________________________________________________
Laurie Frederick, A.SC.T. PAPRICAN
Corrosion Control Group 3800 Wesbrook Mall
The Pulp and Paper Research Vancouver, B.C.
Institute of Canada Canada V6S 2L9

Email: frederick_laurie-at-vanlab.paprican.ca
Tel: 604-222-3200 Fax: 604-222-3207




From: Robert Kayton,MAC,CROET :      kayton-at-ohsu.edu
Date: Fri, 31 Mar 1995 07:04:20 -0800
Subject: TEM: Gold particle counting

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Reply to: RE} TEM: Gold particle counting
Hi Bob,
Your colleague may be suffering from low contrast images. Try acquiring or
scanning in the images with greater contrast range. The contrast of the images
should be such that the black gold particles should be black (an extreme gray
level) and everything else in the image should be a different gray value
(mid-gray to white). For the images that are already digitized try doing a
"Sharpen" or an "Unsharp Masking" operation on the image.

Sincerely,

George McNamara
Universal Imaging Corporation
--------------------------------------

I have colleague that needs help setting up an automatic computer based method
to count gold particles on TEM micrographs. He has NIH image, a good
Macintosh and can import digital images but is having difficulties getting
accurate counts of the gold particles. If anyone has experience with this
please let me know.

Bob Kayton
kayton-at-ohsu.edu







From: Jose.Feijo-at-bio.fc.ul.pt (Jose Feijo)
Date: Fri, 31 Mar 1995 16:44:18 +0000
Subject: Confocal Red line/immuno-labeling in plant tissue

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Message-Id: {9503311445.AA01865-at-dnagel.bio.fc.ul.pt}
X-Sender: bjfeijo-at-skull.cc.fc.ul.pt
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I there:
One of our users has plans to look at entire styles and to do some immuno
labeling of the stylar channel proteins. He wants to get rid of embedding
and/or freeze-sectioning so he figured confocal would be the answer. Among
others, I antecipate some problem with self fluorescence coming either from
chlorophyls and/or lignins. One way around these would be to use the red
line of the Kr-Ar (we have a MRC-600). So, does anyone has experience on
working with secondary antibodies (in this case against chicken) labeled
with red excitable fluorophores in plant tissues? What may the major
drawbacks in this kind of preparation?
Any help would be highly appreciated.


___________________________________________________________
Jose A. Feijo
Dept. Biologia Vegetal, Fac. Ciencias Lisboa
Ed. C2, Campo Grande, P-1700 LISBOA, PORTUGAL

t. + 351.1.7500069 fax + 351.1.7597716
e.mail Jose.Feijo-at-bio.fc.ul.pt
URL: http://www.fc.ul.pt/departs/biologia_vegetal/ejf.html
___________________________________________________________





From: Brian J. Zande :      bz0c+-at-andrew.cmu.edu
Date: Fri, 31 Mar 1995 11:04:56 -0500 (EST)
Subject: New home for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Message-ID: {IjT2UcW00iV_A3idsG-at-andrew.cmu.edu}

The Carnegie Mellon Research Institute (a division of Carnegie Mellon
University) is trying to find a new home for a 13 year old ISI SS-40
SEM. If anyone out there is interested please send mail to me at
bz0c+-at-andrew.cmu.edu

Thanks
Brian




From: Beth Trend :      trend-at-cems.umn.edu
Date: Thu, 30 Mar 1995 15:06:07 -0600
Subject: TEM: Cryo tem of colloids

Contents Retrieved from Microscopy Listserver Archives
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} From the Characterization Facility at the University of Minnesota

MASTER CLASS

Cryo-TEM of Colloidal Materials

=A5 Specimen Preparation

=A5 Cryo-Transfer to the Electron Microscope

=A5 Low-Electron Dose Image and Diffraction Pattern Recording on Film

=A5 Image-Processing and Analysis of Digital Cryo-TEM Images


May 18-20, 1995

Intended Audience

This Master Class is intended for any materials scientists or process eng=
ineers =

working with biological or colloidal materials. This class will explore t=
he use =

of the technique of cryo-transmission electron microscopy to probe the =

microstructure of these samples in the vitrified, hydrated state at resol=
utions =

around 1 nm. Previous TEM experience, although welcome, is not required.=



Description

The lectures will introduce the basics of cryo-transmission electron micr=
oscopy,
including sample preparation and microscopy techniques. Applications to s=
pecific
biological and colloidal materials=D5 samples will be demonstrated, and =

complementary characterization techniques will be described.

The details of sample preparation, focussing on vitrification parameters =
and =

transfer to the microscope, will be described and demonstrated. Imaging =

techniques and interpretation, artifact identification, and quantitative =

analysis will also be covered.

Supervised hands-on laboratories demonstrating the techniques that were =

introduced in the lectures will be interspersed throughout the class. Po=
ssible =

topics include: low dose imaging of soft latex particles; low dose imagi=
ng of =

phospholipid vesicles; image processing of cryo-TEM images using NIH Imag=
e and =

Digital Micrograph; CEVS sample preparation; and video-enhanced light mic=
roscopy
of colloidal materials.

Upon completion of the class, participants will be able to select an appr=
opriate
cryogen, prepare vitrified thin films of colloidal specimens, transfer th=
ese =

specimens to the microscope, and record images free from artifacts caused=
by =

electron beam damage.

Registration

For more information, reply to Beth Trend, trend-at-cems.umn.edu


Instructors

Frank Booy is a Senior Staff Fellow, National Institute of Arthritis =

Musculoskeletal and Skin Diseases at the National Institutes of Health. =
As a =

special expert in electron microscopy, he has worked in the field of =

cryo-electron microscopy of biological materials since 1978, at the CNRS =
in =

Grenoble, France, the European Molecular Biological Laboratory in Heidelb=
erg, =

Germany, and the Rijksuniversiteit Groningen in the Netherlands. Dr. Boo=
y is =

particularly involved in the use of cryo-electron microscopy and 3-D imag=
e =

reconstruction techniques to localize the proteins that make up the =

nucleocapsids of herpes simplex virus.


John Minter, a Research Associate in the Analytical Technology Division o=
f the =

Eastman Kodak Company, is the technical group leader of the Quantitative =
Colloid
Microscopy Group. Minter=D5s group applies cryo-TEM and electron crystall=
ography =

to the study of photographic colloids. During 1990, he was an Industrial =
Fellow =

at CIE collaborating with Professors H. Ted Davis and L. E. Scriven to st=
udy =

surfactant morphology and crystal precipitation by cryo-TEM. He is curren=
tly an =

Adjunct Professor at CIE and Industrial Co-chair of the CIE Characterizat=
ion =

Facility Advisory Committee.

David P. Siegel is a Senior Scientist in the Research & Development Dept.=
, =

Procter & Gamble Co. His general interest areas are membrane biophysics =
and the
physical chemistry of biologically relevant lipids. Dr. Siegel is partic=
ularly =

interested in the mechanisms of biomembrane fusion and of lamellar-to-inv=
erted =

phase transitions in phospholipids. Since 1991, he has used cryo-TEM and=
=

time-resolved cryo-TEM to image intermediates in these processes.

Schedule

Thursday May 18 =

8:00 a.m. Registration =


8:30 Lecture I: Introduction to Cryo-TEM
=A5 Basics steps in cryo-TEM
=A5 Biological and colloidal problems that can be solved using c=
ryo-TEM =

=

10:15 Lecture II: Sample Preparation, Vitrification, Storage, and Trans=
fer to =

the Microscope
=

Lunch =


1:00 p.m. Lab Session - Shepherd Labs

Dinner

7:00-10:00 Lab Session continues as needed
=


Friday, May 19

8:00 Coffee =


8:30 Lecture III: Imaging in Cryo-TEM
=A5 Operation of Electron Microscope
=A5 Recognizing the good samples to examine
=

10:15 Lecture IV: Image interpretation, processing, and analysis
=A5 Artifacts - a rogue's gallery
=A5 Quantitative analysis

11:45 Discussion Session

Lunch

1:00- 6:00 p.m. Lab Session - Shepherd Labs


Saturday, May 20

9:00 a.m.-1:00 p.m. Optional Laboratory Session - Students' samples





____________________________________________________________________=
___
Beth Trend trend-at-cems.umn.edu (faster) or btrend-at-maroon.tc.=
umn.edu
Coordinator, Characterization Facility University of Minnesota
Center for Interfacial Engineering =

100 Union St SE Room 187 phone 612-624-1365 fax 612-626-7530=

Minneapolis, MN 55455 =






From: Brian J. Zande :      bz0c+-at-andrew.cmu.edu
Date: Fri, 31 Mar 1995 12:08:00 -0500 (EST)
Subject: new home for ISI

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Message-ID: {cjT3Pka00iVG44Uk17-at-andrew.cmu.edu}

The Carnegie Mellon Research Institute (a division of Carnegie Mellon
University) is trying to find a new home for a 13 year old ISI SS-40
SEM. If anyone out there is interested please respond to me at
bz0c+-at-andrew .cmu.edu

Thanks,
Brian




From: MicroToday-at-aol.com
Date: Sat, 1 Apr 1995 08:58:29 -0500
Subject: Microscopy Events

Contents Retrieved from Microscopy Listserver Archives
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In our newsletter, Microscopy Today, we attempt to publish monthly an
on-going summary of ALL microscopy events (conferences/meetings, schools,
training sessions, etc.) in the U.S. - plus major international events.
A no-charge listing includes:
Dates
Title
Sponsor/Organizer
Location
Contact/Tel/Fax
We will consider longer write-ups and ask for an article contribution to the
newsletter, rather than money, for the effort.
The newsletter is mailed only to those who have requested copies - at no
charge currently to some 8,000 microscopists in the U.S., Canada and the U.K.
Unfortunately we must charge a modest subscription for other international
readers.
We invite your no charge event listing - or request for a subscription.
Regards,
Don Grimes, Editor





From: Juranyi Zsolt :      juranyi-at-kokiux.koki.hu
Date: Sat, 1 Apr 1995 15:53:26 +0200 (MET DST)
Subject: Subscription request

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We would be obliged if you informed us about the specifications
(including ISBN codes) of those books/atlases/workbooks which were
published for students in the field of ULTRASTRUCTURAL HUMAN PATHOLOGY in
the last ten years.
Could you recommend for us some?
Thank you in advance,sincerely yours

Szabolcs Viragh and Evelin Orso





From: MED_SCU-at-FRCU.EUN.EG
Date: Sun, 02 Apr 1995 10:28:59 +0000 (O)
Subject:

Contents Retrieved from Microscopy Listserver Archives
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First Intarnational Conference on Electron Microscopy
and
Advances in Research in Differcent Fields of Sciance
September 1995
Ismailia - Etap

Sponsored by
Electron Microscopy Center
Suez Canal University
Ismailia - Egypt

Special Topics of the conference:
1- Role of EM in diagnostic virology.
2- Role of EM in diagnosis of tumors cylulogy and urinary atones.
3- Role of EM in ultrastructure pathology of the lung (non neoplastic
conditions).
4- X-ray microanalysis: Applications particularly metalllurgical,
mineralogical, and biological.
5- Scanning EM of plants, animal, ineccts, and mineral material.
6- Study of biological macromolecules from their characteristic
electron diffraction patterns.
7- Skin pathology by EM.
8- Morphological ldentification of antigens by EM.
9- Different low temperature methods for biological EM.
10- Safety measures and maintenance needed for EM.

There will be an equipment exblbition in conjunction with this
meeting. Registration for foreigners will be US $ 150 inclusive of
full board during the time of the meeting.
For further information, contact the organizar:
Prof, Dr. Khalifa Ibrahim Khalifa
Electron Microscvpe Center
Suez Canal University
Ismailia - Egypt
Fax: (20) 64- 329478 ( phone and Fax number)
Fax: (20) 64- 333318 ( Phone and Fax number)

Massage from: sayed Mersal





From: tsi-at-werple.mira.net.au (Thomson Scientific:Paul Thomson)
Date: Mon, 3 Apr 1995 12:25:04 +1000
Subject: Newsgroup subsciption

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Please subscribe us to this newgroup.





From: MED_SCU-at-FRCU.EUN.EG
Date: Mon, 03 Apr 1995 12:38:39 +0000 (O)
Subject:

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


First Intarnational Conference on Electron Microscopy
and
Advances in Research in Differcent Fields of Sciance
September 1995
Ismailia - Etap

Sponsored by
Electron Microscopy Center
Suez Canal University
Ismailia - Egypt

Special Topics of the conference:
1- Role of EM in diagnostic virology.
2- Role of EM in diagnosis of tumors cylulogy and urinary atones.
3- Role of EM in ultrastructure pathology of the lung (non neoplastic
conditions).
4- X-ray microanalysis: Applications particularly metalllurgical,
mineralogical, and biological.
5- Scanning EM of plants, animal, ineccts, and mineral material.
6- Study of biological macromolecules from their characteristic
electron diffraction patterns.
7- Skin pathology by EM.
8- Morphological ldentification of antigens by EM.
9- Different low temperature methods for biological EM.
10- Safety measures and maintenance needed for EM.

There will be an equipment exblbition in conjunction with this
meeting. Registration for foreigners will be US $ 150 inclusive of
full board during the time of the meeting.
For further information, contact the organizar:
Prof, Dr. Khalifa Ibrahim Khalifa
Electron Microscvpe Center
Suez Canal University
Ismailia - Egypt
Fax: (20) 64- 329478 ( phone and Fax number)
Fax: (20) 64- 333318 ( Phone and Fax number)

Massage from: sayed Mersal





From: szabop-at-bmeik.eik.bme.hu
Date: Mon, 03 Apr 1995 17:21:52 +0100
Subject: Subscription request

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html








From: DCROMEY-at-CCIT.ARIZONA.EDU
Date: Mon, 03 Apr 1995 08:22:29 -0700 (MST)
Subject: Re: Subscription request

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Szabolcs and Evelin,
I have several recommendations for you (though I'm sorry I don't have the
ISBN codes handy). I would recommend the Journal: Ultrastructural
Pathology. I would also recommend becoming a member of the
Ultrastrucural Pathology Society. This society has among its members
some of the "giants" in this field. To join, or inquire for more
information please contact:

Claire M. Payne, Ph.D.
Secretary, Ultrastructural Pathology Society
University of Arizona
Dept. of Microbiology & Immunology
1501 N. Campbell
Tucson, AZ 85724
USA

Books:

Diagnostic Ultrastructure of Non-Neoplastic Diseases (1992),
Papadimitriiou, Henderson and Spagnolo,
Churchill Livingstone, Pub.

Diagnostic Electron Microscopy: A Text/Atlas (1988), Dickerson,
Igaku-Shion, Pub.

Ultrastructural Appearances of Tumors (1986), Henderson, Papadimitriou,
Coleman, Churchill Livingstone, Pub.

Diagnostic Ultrastructural Pathology (????) Dvorak, Monahan-Earley, CRC
Press

Ultrastructural Pathology of the Cell and Matrix (1988) Ghadially,
Butterworths, Pub.

This is by no means an exhaustive list, but I hope its a start.

Doug Cromey
************************************************
On Sat, 1 Apr
1995, Juranyi Zsolt wrote:

} We would be obliged if you informed us about the specifications
} (including ISBN codes) of those books/atlases/workbooks which were
} published for students in the field of ULTRASTRUCTURAL HUMAN PATHOLOGY in
} the last ten years.
} Could you recommend for us some?
} Thank you in advance,sincerely yours
}
} Szabolcs Viragh and Evelin Orso
}
}


Douglas W. Cromey, M.S.
Cell Biology and Anatomy
Arizona Health Sciences Center
1501 N. Campbell Ave.
Tucson, AZ 85724
(520)626-2824 dcromey-at-ccit.arizona.edu













From: JONES-at-KRDC.INT.ALCAN.CA
Date: Mon, 03 Apr 1995 12:01:08 -0500 (EST)
Subject: subscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html






From: sfzane-at-unccvm.uncc.edu (Sandra F. Zane)
Date: Mon, 3 Apr 1995 12:42:40 -0400
Subject: Re: subscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I see several folks trying to subscribe to the Fatfree list using the wrong
address. This is the subscription address: FATFREE-REQUEST-at-HUSTLE.RAHUL.NET

To join, send e-mail to the subscription address using one of the following
subjects:
ADD to join as a regular member
ADD DIGEST to join as a digest member

Hope this helps.

At 12:01 PM 4/3/95, JONES-at-KRDC.INT.ALCAN.CA wrote:
}
Sandra F. Zane, EM Tech.
Biol. Dept. UNCC
Charlotte, NC 28223
sfzane-at-unccvm.uncc.edu
Fax (704) 547-3128





From: tivol-at-tethys.ph.albany.edu
Date: Mon, 03 Apr 1995 13:12:17 EDT
Subject: Re: SEM: Carbon Evaporators

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Dear Laurie,
We have an old Ladd and a couple of home-made evaporators. The Ladd
has been here longer than I (15 years), gets heavy use, and still works just
fine (we just use it for coating grids, etc., so your mileage may vary). We
use the 1/8" rods narrowed to ~1 mm (reduced section). I have coated objects
from barely visible to very dark in one shot. BTW, a piece of paper folded
so that one part shadows the other allows the color of the carbon to be fol-
lowed relatively easily once one gets the hang of it. Any bell jar can, of
course, be combined with the evaporating system (power supply, rod holder,
etc.), so if you have the pumps available, you might save some $ by buying
parts. Good luck.
Yours,
Bill Tivol




From: Rebecca K Siegel-Wasserman :      SIEGELWA-at-AC.GRIN.EDU
Date: Mon, 03 Apr 1995 13:31:02 -0500 (CDT)
Subject: unsubscribe

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please unsubscribe me
siegelwa-at-ac.grin.edu




From: tivol-at-tethys.ph.albany.edu
Date: Mon, 03 Apr 1995 12:48:06 EDT
Subject: Re: advises about filament needed

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Dear Xiaogang,
We have used both new and rebuilt W filaments, and have found the re-
built ones to be at least as good as the new ones--in some ways better. The
rebuilt ones we bought were from EBTEC (I am only a customer & have no finan-
cial interest). The advantages of EBTEC's designs are 1) the connection of
the hairpin to the posts is better than on some other designs, and 2) they have
"regular" and "sharp" tips. The regular tips give a nice bright beam, and the
sharp ones give a smaller source size. The intensity per unit area for the
sharp tips is about the same as that for the regular tips, so the total inten-
sity is less; however, the coherence is better, so for diffraction with radia-
tion-sensitive specimens, the sharp tips are very good.
We routinely preheat our filaments before mounting them in the Wehnelt
cylinders. I don't know what the thin film you find sticking to your filaments
is, but maybe preheating will help. Good luck.
Yours,
Bill Tivol




From: Steve Miller
Date: 03 Apr 95 14:19:25 EDT
Subject: Carbon evaporation

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With 20 years of experience in servicing DV502's the most common problems I
have encountered with carbon evaporation are:

1. Early units did not have a braided conduction wire between the moveable
collet and the frame for sure current conduction. The fix for this is get
the braid and drill/tap holes for fastening. If this is a problem, contact
Denton to send it in for upgrade.

2. All versions work well with reduced section rods (approx .7mm diameter),
pointed rods vary in conduction from start to finish and when the size
nears the full 2mm the current can well go over 50 amps. I find reduced
section rods will evaporate between 20-30 amps.

3. If the fuse is blown, often the fuse is replaced with a standard fuse.
The correct fuse would be a ceramic type ABC fuse which is more tolerant
and safer when it blows.

4. With reduced section rods if evaporation is continued past the reduced
section you get the same overcurrent as with pointed rods.

5. Unfortunately there is a lot of variance in the way carbon is made into
rods. You will see a variance of color, brittleness, ash formation (from
some filler!). Only buy small quantities of any type until you find one
that is good.

Obviously constant pressure on the rods during evaporation is essential.
The leaf spring used for pressure can be easily abused by pulling it
straight back to put in carbon rods. A better way of displacing it is to
push it down for loading the carbon rod. If there is poor tension you
really have to remove the spring and reform it to put more pressure on the
rod.

7. If all of this doesn/t help, call the company and GET HELP, there is no
reason to put up with poor performance, all parts and advice are available.
The phone at Denton is 609-439-9100.

For any more detailed discussions you may contact me at Integrated
Microsystems, Inc. 708-698-4210, fax 708-696-2541 or email.




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Mon, 3 Apr 1995 15:37:05 -0500 (CDT)
Subject: Enough of Egyptian Announcement

Contents Retrieved from Microscopy Listserver Archives
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Colleagues...

I don't want to sound like I'm beating down
the Egyptian meeting. But we've had multiple
postings of that announcement for the last few weeks.
I think we've had enough. Unfortunately, they
are from different sources every time. So I cannot
touch base with one person/organization and
ask them to stop. So as a general announcement

Please cease posting this announcement until
there is a significant change!

Thanks...

Nestor
Your Friendly Neighborhood SysOp.








From: :      MELLIOTT-at-prl.pulmonary.ubc.ca
Date: Mon, 3 Apr 1995 15:18:39 +0800PST
Subject: diamond knives

Contents Retrieved from Microscopy Listserver Archives
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I remember seeing a request in the last few weeks about diamond
knives. We are about to purchase one and are looking for suppliers
and any thoughts on who makes the best diamond knives. I seem to
remember the last time I bought one that the best were from Dupont or
Diatome(?) but can't remember exactly which. Is that still true.?
Any help greatly appreciated.

Yours
Mark Elliott PhD




From: Heinz Hemken :      hhemken-at-cell.cinvestav.mx
Date: Mon, 3 Apr 1995 16:17:19 -0700 (PDT)
Subject: Re: Enough of Egyptian Announcement

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I've had a similar problem with this list that I haven't had with others.
My postings get to the list, but somewhere out there multiple copies
bounce off servers and come back over a period of days. Not being a UNIX
guru, I have no idea why this is.

------------------------------------------------------------------
Heinz Hemken
Departamento de Biologia Celular, CINVESTAV-IPN
http://cell.cinvestav.mx/bchh.html

On Mon, 3 Apr 1995, Nestor J. Zaluzec-Argonne Nat. Lab. wrote:

} Colleagues...
}
} I don't want to sound like I'm beating down
} the Egyptian meeting. But we've had multiple
} postings of that announcement for the last few weeks.
} I think we've had enough. Unfortunately, they
} are from different sources every time. So I cannot
} touch base with one person/organization and
} ask them to stop. So as a general announcement
}
} Please cease posting this announcement until
} there is a significant change!
}
} Thanks...
}
} Nestor
} Your Friendly Neighborhood SysOp.
}
}
}
}




From: Andreh Khachatouri :      usd14716-at-interramp.com
Date: Mon, 3 Apr 1995 22:01:06 -0800
Subject: Fixatives In electron microscopy

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X-Mailer: InterCon TCP/Connect II 2.1.2
Mime-Version: 1.0
Message-Id: {9504032201.AA06079-at-usd14716.interramp.com}

I am working with sea urchin eggs and would like to have pictures of untreated
eggs plus treated eggs ( with DTT and alpha-amylase) with SEM and TEM. Iam not
sure what fixatives are appropriate for SEM and TEM . Please help me.





From: Andreh Khachatouri :      usd14716-at-interramp.com
Date: Mon, 3 Apr 1995 22:09:38 -0800
Subject: Fixatives in Electron microscopy

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X-Mailer: InterCon TCP/Connect II 2.1.2
Mime-Version: 1.0
Message-Id: {9504032209.AA38846-at-usd14716.interramp.com}

I would like to know what fixatives to use to have pictures of sea urchin eggs
for SEM and TEM. Thanks





From: Stephan Helfer :      STEPHAN-at-rbge.org.uk
Date: Tue, 4 Apr 1995 13:20:55 BST
Subject: Subscription to AMC microscopy mailing list

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Dear Dr Zaluzek

I have tried in vain to contact you both using
Zaluzek-at-aaem.amc.anl.gov and listserver-at-....; I hope I'm getting
through this time.

Could you please add my name and Email address to the AMC
microscopy mailing list.

Thanks very much

Yours sincerely


Dr Stephan Helfer, SSO
Mycologist

Royal Botanic Garden Edinburgh, Inverleith Row, EDINBURGH EH3 5LR,
Scotland UK

email STEPHAN-at-rbge.org.uk
phone: +44 (0)131 552 7171 ext 280
or +44 (0)131 459 0446-280 (direct digital VoiceMail line)
fax: +44 (0)131 552 0382




From: Fangl-at-fpms.fpms.ac.be
Date: Tue, 4 Apr 1995 17:36:42 +0200
Subject: Re: Microscopy Research and Technique, Guest Editors

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Dr. Johnson: WOuld you mail me already existed topical set about material sc
science structure. Thanks!
FANG L.




From: W.L. Steffens :      STEFFENS.B-at-calc.vet.uga.edu
Date: Tue, 4 Apr 1995 09:35:35 EST
Subject: Sea Urchin Eggs

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To Andreh Khachatouri:

I did a considerable amount of TEM and SEM on urchin eggs a number of
years ago. As I recall, the fixative giving the best results was
something like 2% Glut. in seawater followed by osmium post-fixation. For
references, see Byrd and Eppel, and Belisle and Byrd from the period of
1974 to 1979. Sorry I can't be more specific.

-=W.L. Steffens=-
College of Veterinary Medicine
University of Georgia




From: sfzane-at-unccvm.uncc.edu (Sandra F. Zane)
Date: Tue, 4 Apr 1995 09:55:02 -0400
Subject: apology for error

Contents Retrieved from Microscopy Listserver Archives
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Good Morning Microscopists,
Yesterday morning after observing several folks trying to subscribe to
this list using the wrong address and some folks attempting to do the same
with another list, I thought I would be helpful. Well....I somehow got my
wires crossed and it seems that a great many of you now know how to join
that other list.
Please accept my apologies and I promise that I shall never again
attempt to be helpful on a Monday morning. (Hopefully, I provided a chuckle
for some of you.) Sandra
Sandra F. Zane, EM Tech.
Biol. Dept. UNCC
Charlotte, NC 28223
sfzane-at-unccvm.uncc.edu
Fax (704) 547-3128





From: DCROMEY-at-CCIT.ARIZONA.EDU
Date: Tue, 04 Apr 1995 07:50:58 -0700 (MST)
Subject: Re: diamond knives

Contents Retrieved from Microscopy Listserver Archives
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Mark,
I have had good luck, good quality and quick turn around from Micro
Engineering (409)291-6891. My purchases were only resharpennings of old
DuPont knives, but the price was good and so was the quality. Some of
the folks I work with now will only buy Diatome diamond knives.
Hopefully that means that almost any major manufaturer will have some
high quality knives available.
Doug


Douglas W. Cromey, M.S.
Cell Biology and Anatomy
Arizona Health Sciences Center
1501 N. Campbell Ave.
Tucson, AZ 85724
(520)626-2824 dcromey-at-ccit.arizona.edu













From: Suichu Luo :      SUICHU-at-utkvx.utk.edu
Date: Tue, 04 Apr 1995 14:52:49 -0500 (EST)
Subject: subscribe

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Dear Sir:

Would you please send me the information.

Thanks a lot.

Suichu Luo




From: chandler-at-lamar.ColoState.EDU (John Chandler)
Date: Tue, 4 Apr 1995 16:59:52 -0700
Subject: Freeze-Fracture Short Course Announcement

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The Electron Microscopy/Imaging Center at Colorado State University will be
offering a 5 day Short Course in Freeze-Fracture Techniques this summer.
In addition to comprehensive lectures, state-of-the-art instruments will be
available for hands-on training in the laboratory.

For more detailed information, please visit our World Wide Web site at URL:
http://www.vetmed.colostate.edu/anatomy/emic/homepage.html, or contact me
directly (not to this list) for an email copy of the announcement.


John
chandler-at-lamar.ColoState.EDU
http://www.vetmed.colostate.edu/anatomy/faculty/chandler.html






From: Ann-Fook Yang :      YANGA-at-EM.AGR.CA
Date: Tue, 04 Apr 1995 16:48:21 -0400
Subject: Re:Diamond knives

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Mark Elliott asked about diamond knives.
Du Pont sold their diamond knife business to Delaware Diamond
Knives, Inc. years ago. I don't know the further development.
We have several users here; all use Diatome knives and all are
satisfied with their knives. One of the users sent a Diatome
diamond knife, which had been resharpened by a competitor, back to
the Diatome factory for resharpening several years ago. Diatome
would not touch it because it was not one of theirs, although the
boat was. The diamond was of lower quality. This indicates their
high standard of quality.
There may be other good diamond knives in the marketplace, but you
can't go wrong with a Diatome knife. BTW I have nothing to do
with the Diatome except using their knives.

Ann Fook Yang





From: erich-at-ento.csiro.au
Date: Wed, 5 Apr 1995 00:09:54 +1000
Subject: McIlvaine's Buffer

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Message-Id: {9504050210.AA08736-at-spider.ento.csiro.au}

Dear All,
Does anyone have a recipe for McIlvaine's Buffer which I'm told is for pH3
to pH6 and is used for amylase assays? Many thanks,
Eric Hines





From: W.L. Steffens :      STEFFENS.B-at-calc.vet.uga.edu
Date: Tue, 4 Apr 1995 09:29:07 EST
Subject: diamond knives

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To Mark Elliot:

For the past 12 years, I have been dealing exclusively with
MicroEngineering Inc. who make the Microstar diamond knife. I have no
interest in this company other than the fact that they make a high
quality, affordable product. In the rare cases when I have received a bad
one, I have had a replacement by the next day or 2. Pricewise, I would
have difficulty justifying any other brand to my procurement department.
Again, this is no sales pitch, but just the way its been with my lab which
buys or resharpens 1 or 2 knives a year.

-=W.L. Steffens=-
College of Veterinary Medicine
University of Georgia




From: SUSAN R. SESACK :      SESACK-at-brain.bns.pitt.edu
Date: Tue, 4 Apr 1995 09:10:15 EDT
Subject: Re: diamond knives

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} From: {MELLIOTT-at-prl.pulmonary.ubc.ca}
} Organization: UBC Pulmonary Research Lab
} To: microscopy-at-aaem.amc.anl.gov
} Date: Mon, 3 Apr 1995 15:18:39 +0800PST
} Subject: diamond knives
} Priority: normal

} I remember seeing a request in the last few weeks about diamond
} knives. We are about to purchase one and are looking for suppliers
} and any thoughts on who makes the best diamond knives. I seem to
} remember the last time I bought one that the best were from Dupont or
} Diatome(?) but can't remember exactly which. Is that still true.?
} Any help greatly appreciated.
}
} Yours
} Mark Elliott PhD
}

Mark,

I have always used Diatome diamond knives, as did the lab where I was
trained. They are of superior quality and can be sharpened as often
as needed, for life. Other companies sometimes limit the number of
sharpenings that they will guarantee. I also have always had
exceptional help and service from Stacey Kirsch who heads the US
division of Diatome. She can be reached at 800-523-5874 for current
pricing and additional information.

S.
sesack-at-bns.pitt.edu
Susan R. Sesack
Dept. Neuroscience
University of Pittsburgh
Pittsburgh, PA 15260




From: Peter D. Barnett :      pbarnett-at-crl.com
Date: Tue, 4 Apr 1995 13:52:02 -0700 (PDT)
Subject: Seminar of California Association of Criminalistics

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CALIFORNIA ASSOCIATION OF CRIMINALISTS
MEETING ANNOUNCEMENT

The California Association of Criminalists (CAC) is holding
its 85th Semi-Annual meeting on May 10-13, 1995, at the Walnut
Creek Marriott in Walnut Creek, California. The Contra Costa
County Sheriff's Department Criminalistics Laboratory will be
hosting this meeting.

The program is shaping up fast. Some of the subjects planned
include: the Integrated Ballistics Identification System
(IBIS), computer animation in casework, retrieving secured data
in cases of computer crime, and bite mark analysis in the case
of a mountain lion attack. This is also our last call for
papers so this is your chance to share your latest project or
most interesting case. Both your technical notes and innovative
research are welcome. For an abstract form contact Rich Schorr,
voice (510)646-2455 or fax (510)646-2913.

Workshops planned include: A Computer Workshop for Cyber-
phobes (Everything You Ever Wanted To Do But Were Afraid To
Admit You Didn't Know How), Practical Applications of GCMS
In a Crime Laboratory Environment, a Glock Armorers Course,
DNA Users Group Meeting and a Polaroid Film Product Workshop.

For more information or to receive a registration package
contact Karen Sheldon, Contra Costa County, Sheriff-Coroner's
Department, 1122 Escobar St., Martinez, CA 94553, voice (510)
646-2455 or fax (510)646-2913.






From: richard.easingwood-at-stonebow.otago.ac.nz (Richard Easingwood)
Date: Wed, 5 Apr 1995 16:38:16 +1100
Subject: Bacteria cryosubstitution

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X-Sender: st004718-at-brandywine.otago.ac.nz
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Dear Cryo Electronmicroscopists,
I have a student who wants to compare the quantity of extracellular
polysaccharide material produced by bacterial cells grown on agar plates
(we don't want to use cells from broth) by TEM. The bacteria are involved
in plant nodule formation.
We would like to loose as little of the material as possible during
processing. I think plunge freezing with a KF80, cryosubstituting the cells
using perhaps 2% OsO4 in methanol would be good, followed by conventional
dehydration and embedding in epoxy. Conventional room temperature fix
methods use glutaraldehyde/ruthenium red/OsO4 in various concentrations to
fix and stain the extracellular material but from the pictures I've seen I
suspect much of the material has still been lost.

Is cryoprotection of the sample necessary? If I lightly fix then infiltrate
in sucrose or some other protectant I'm a bit worried we'll start washing
the polysaccharide away, if I don't then the cells (which will be a small
scraping of colonies off the plate surface) could be ice damaged.
If I don't use ruth. red as a stain will the material still be visible -
even if present?
I'd be grateful to hear from anyone who has had experiance of similar samples.

Regards,
Richard E

Richard Easingwood
South Campus Electron Microscope Unit
Otago Medical School
PO Box 913
Dunedin
NEW ZEALAND

Telephone: 64-03-479 7301
Facsimile: 64-03-479 7254

"The southernmost electron microscope unit in the world"






From: tivol-at-tethys.ph.albany.edu
Date: Tue, 04 Apr 1995 12:59:30 EDT
Subject: Re: pointed filaments

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Dear John,
You asked about specific improvements in capabilities vs reduced life-
times for pointed filaments in use with radiation-sensitive specimens. First,
let me say that we experienced no reduction in lifetime. Since, with proper
design, a pointed filament can be saturated at a lower current, this is not
too surprising. In my work--as opposed to nearly everyone else I know--I often
try to get rid of electrons. For EDX and crystallography I operate at the low-
est setting for wehnelt bias, a maximally excited first condenser lens, and a
small-bore condenser aperture. If I use a more intense beam for EDX, the dead-
time of my system and my beam spot both get larger. For crystallography, I
scan the specimen using low beam currents to avoid damage during the time I am
not recording (damage during recording is inevitable). Furthermore, when I
scan in diffraction mode, I want the beam size to be the same as the selected
area, so that, again, every electron incident on the specimen is of some use.
Bearing these facts in mind, the smaller beam size from a pointed fil-
ament reduces the brehmsstrahlung radiation from the condenser aperture and
other sources within the column and increases the signal-to-noise ratio and
spatial resolution for microanalysis (not that my spatial resolution is too
good; with a high-voltage EM and thick sections, the analysis volume will
never be small). Exposure of unexamined areas of a specimen is crystallography
is harder to characterize; however, given that scanning the specimen, tweaking
the position and adjusting the beam, etc. take only a few percent of the expo-
sure recorded on film, and that often several ED patterns can be taken from
the same selected area, there would seem to be little effect on the data. It
must be remembered that the ultimate, irreducible limit to the data obtainable
is set by damage to the specimen and that the higher-resolution reflections are
the most sensitive to this damage. Once again, it is the smaller size of the
beam which is beneficial. Additionally, the ED pattern is better when the ob-
jective lens is focussed (although, in the HVEM, this is not too critical),
and focussing can be accomplished very rapidly using minimum contrast, which
works better with a more coherent beam.
I have not done quantitative comparisons between regular and pointed
filaments for either case, so I can't give you specific figures of merit, but
I can tell you that the beam is visibly smaller and the interference fringes
are noticably more prominant with the pointed filament. I also have no idea
whether any of these considerations are specific to 1.2 MV electrons, although
I wouldn't think so.
Another occasional consideration is that heating and charging effects
are minimized at low beam currents, so the pointed filaments are more specimen-
friendly in this way also.
Yours,
Bill Tivol




From: Ann-Fook Yang :      YANGA-at-EM.AGR.CA
Date: Wed, 05 Apr 1995 12:23:37 -0400
Subject: Re:Diamond knife quality

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Message-ID: {1452822F0179AEAA-at-ggpl.arsusda.gov}

I mentioned in my last message about DIATOME would not touch the
knife which had been resharpened by a competitor. I should have
made it clear that "the diamond was of lower quality" was a direct
quote from a sales rep.

Ann Fook Yang





From: South Bay Technology, 73531,1344
Date: 05 Apr 95 12:18:19 EDT
Subject: Copy of: Polaron Polabed 812

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---------- Forwarded Message ----------


I POSTED THIS MESSAGE SEVERAL WEEKS AGO AND RECEIVED NO RESPONSE. I THOUGHT I'D
GIVE IT ANOTHER TRY JUST IN CASE THERE WAS A DELIVERY PROBLEM.

RE: Copy of: Polaron Polabed 812

Dear Microscopists-

I was wondering if anyone out there knows what happened to the Polaron line of
products. I am particularly curious about some of their embedding resins such
as the Polabed 812.

Thanks for your help!

David Henriks
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673

TEL: 714-492-2600
FAX: 714-492-1499





From: bergrh-at-cc.memphis.edu (R. Howard Berg)
Date: Wed, 05 Apr 1995 08:22:09 +0600
Subject: Re: Bacteria cryosubstitution

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Richard Easingwood writes:

} Dear Cryo Electronmicroscopists,
} I have a student who wants to compare the quantity of extracellular
} polysaccharide material produced by bacterial cells grown on agar plates
} (we don't want to use cells from broth) by TEM. The bacteria are involved
} in plant nodule formation.
} We would like to loose as little of the material as possible during
} processing.

Cryofixation would be a good way to go. Why not freeze sub in
osmium/acetone, embed in epoxy, as normal, and then do a PAS stain on
sections? The osmium and normal poststains are unlikely to stain all the
polysaccharides. It would be interesting to find out if freeze
substitution in ruthenium red/acetone would work (i.e., would the RR
penetrate?).


R. Howard Berg, Ph.D.
Biology Department
University of Memphis
Memphis, TN, 38152
E mail: bergrh-at-cc.memphis.edu
phone: 901-678-4449 fax: 901-678-4457







From: A. Kent Christensen :      akc-at-umich.edu
Date: Wed, 5 Apr 1995 10:42:26 -0400 (EDT)
Subject: Re: diamond knives

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Message-Id: {9504051952.AA10641-at-esds01.es.dupont.com}

Continuing the diamond knife theme, I have been using Diatome knives
for many years, and have been impressed with their quality and reliabity.
But in the commercial exibits at the Cell Biology meetings in San
Francisco last December, I became aware of diamond knives being made in
the Netherlands by Drukker International, B.V., an old dutch diamond firm
(dating from 1906). The descriptions sounded quite impressive, including
"superb wetting behavior". A 3 mm blade knife costs $2500. Or you can
use their "exchange program" = if you send them an old diamond knife (any
manufacturer, any condition), you will receive a new 3 mm Drukker knife
for $1750 (if the new knife isn't shipped within two weeks, then you get
it free). The U.S. distributor is Harris Diamond Corporation, 100 Stierli
Court, suite 106, Mount Arlington, N.J. 07856, tel (201) 770-1520, fax
(201)770-1549. I don't know anything about the company other than
spending a few minutes at their booth at the meetings and receiving a nice
brochure. If I had plenty of money and were buying a diamond knife today,
I would probably buy Diatome, but I thought EM people would want to be
aware of the Drukker knife, which could be very good.




From: richard.lander-at-stonebow.otago.ac.nz (Richard Lander)
Date: Thu, 6 Apr 1995 10:10:16 +1200
Subject: SEM of microcapsules

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To listserver people,
Many thanks for the replies to my problem.
We found that putting the Microcapsules between 2 squares of double sided
tape and tearing them apart, left a good idea of the internal structure.
Pretty simple really! This was adequate for what the user was wanting and
they were pleased with the results. So pleased, they have decided to bring
back about 80 samples!

rich.

*****************************************************************
* Richard Lander *
* South Campus Electron Microscope Unit *
* c/- Pathology Department *
* Otago Medical School *
* P.O. Box 913 *
* Dunedin *
* N.Z. *
* *
* Tel. National 03 479 7301 International 64 3 479 7301 *
* Fax. National 03 479 7254 International 64 3 479 7254 *
*****************************************************************






From: David Garrett :      DGARRETT-at-gab.unt.edu
Date: Wed, 5 Apr 1995 08:32:17 CST6CDT
Subject: Re: diamond knives

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} From: "W.L. Steffens" {STEFFENS.B-at-calc.vet.uga.edu}
} Organization: College of Vet. Med
} To: Microscopy-at-aaem.amc.anl.gov
} Date sent: Tue, 4 Apr 1995 09:29:07 EST
} Subject: diamond knives
} Priority: normal

} To Mark Elliot:
}
} For the past 12 years, I have been dealing exclusively with
} MicroEngineering Inc. who make the Microstar diamond knife. I have no
} interest in this company other than the fact that they make a high
} quality, affordable product. In the rare cases when I have received a bad
} one, I have had a replacement by the next day or 2. Pricewise, I would
} have difficulty justifying any other brand to my procurement department.
} Again, this is no sales pitch, but just the way its been with my lab which
} buys or resharpens 1 or 2 knives a year.
}
} -=W.L. Steffens=-
} College of Veterinary Medicine
} University of Georgia
}
MicroEngineering is now known as Micro Star Technologies.
Contact: Cathy Zimmerman (800)533-2509, E-mail US3SNQ7N-at-IBMMAIL.COM
***************************************
David Garrett "DGARRETT-at-GAB.UNT.EDU"
University of North Texas
Dept. Biological Sciences
(817)565-3964 Fax (817)565-4136
***************************************




From: Jim Stanley :      jstanly-at-mse.ogi.edu
Date: Wed, 5 Apr 1995 08:54:14 -0700 (PDT)
Subject: Re: OM Polishing of Al

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Although, its been a while since I've polished aluminum. My initial
impression is you have way too many steps. I'd go from 600 grit to 5
micron, to 1 micron, perhaps the .25 micron and then the colloidal. I
also remember that cerium oxide paste gave better results. (probably not
the greatest idea from a saftey and envirnomental standpoint) THe key to
fine polishing of aluminium is to keep the steps to a minimum and the
length of time at each step to a minimum. There may be a sure bet way of
polishing aluminium....but I found it a real pain.

On Tue, 4 Apr 1995 IE09-at-VM.ACS.UNT.EDU wrote:

} We are currently trying to polish aluminum samples (7075). Before we etch
} them, we are trying to get a 0.05 micron finish. The grinding/polishing
} sequence is: 600 grit, 800 grit, 1200 grit, 3 micron diamond paste, 1 micron
} diamond paste, 0.25 micron paste, 0.05 colloidal silica. Everytime we go from
} the 1 micron step to the 0.25 micron step scratches appear....
}
} We have been using Allied High Tech Spec-Cloth polishing pads. Doeas anyone
} have suggestions to get to the final two steps without introducing scratches?
}
} I do not really understand why they get scratched so easily when the finer
} pastes are used.
}
} Should these samples be stored in a dessicator to prevent oxidation? I have
} never worked with Al, and therefore would appreciate any suggestions.
}
} Patrick Diehl
} Center for Materials Characterization
} University of North Texas







From: IAN HALLETT :      ihallett-at-marccri.marc.cri.nz
Date: Thu, 6 Apr 1995 14:44:38 GMT+1200
Subject: Re: Copy of: Polaron Polabed 812

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} Date: 05 Apr 95 12:18:19 EDT
} From: South Bay Technology {73531.1344-at-compuserve.com}
} To: Microscopy ListServer {MICROSCOPY-at-aaem.amc.anl.gov}
} Subject: Copy of: Polaron Polabed 812


} I POSTED THIS MESSAGE SEVERAL WEEKS AGO AND RECEIVED NO RESPONSE. I THOUGHT I'D
} GIVE IT ANOTHER TRY JUST IN CASE THERE WAS A DELIVERY PROBLEM.
}
} RE: Copy of: Polaron Polabed 812
}
} Dear Microscopists-
}
} I was wondering if anyone out there knows what happened to the Polaron line of
} products. I am particularly curious about some of their embedding resins such
} as the Polabed 812.
}
} Thanks for your help!
}
} David Henriks
} South Bay Technology, Inc.
} 1120 Via Callejon
} San Clemente, CA 92673
}
} TEL: 714-492-2600
} FAX: 714-492-1499

David

I am not sure about specific products but the fate of Polaron goes
Polaron was sold to BioRad who sold it to Fisons. By contacting a US
rep of Fisons you may get more info, although the unit may have been
sold to Thermo Instruments as part of the Fisons scientific
instrument division sale.

As far as Polabed goes a number of suppliers have similar alternative
to EPON 812.

Ian Hallett



Ian Hallett
HortResearch
Mt Albert Research Centre
Private Bag 92 169
Auckland, New Zealand
Fax 64-9-815 4201
Telephone 64-9-849 3660




From: Peter D. Barnett :      pbarnett-at-crl.com
Date: Wed, 5 Apr 1995 22:08:32 -0700 (PDT)
Subject: RE: Daughters at Work Day

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On Wed, 5 Apr 1995, B.A. wrote:

}
} We (DuPont CR&D Analytical) have hosted girls in our lab for the past 2 years
} on Take Your Daughters to Work Day and have set up several demonstrations using
} materials we thought would be familiar to the girls. Lots of parents bend the
} rules and bring quite small girls as young as 6, so be prepared.
}
My High School teacher wife read this over my shoulder and asked me to
remind everyone who has these types of event to have them in the summer
so that the students won't miss school. Teachers who try to do a good
job for classrooms of 25 to 35 students, cannot tailor their lessons to
meet the demands of students who take days off for going to work with mom
or dad, and similar things can can just as easily be done during school
vacation periods.





From: South Bay Technology :      73531.1344-at-compuserve.com
Date: 05 Apr 95 12:53:02 EDT
Subject: OM Polishing of Al

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Patrick-

It is difficult to pinpoint the exact cause for the scratches, but there are a
few things you may want to check.

1) What size are the scratches that you see? Are they significantly larger than
the 1u scratches you are trying to remove? Perhaps you are getting an
agglomeration of .25u particles if they are not properly wetted and dispersed in
the paste. Have you used this same .25u paste before without trouble? Perhaps
you should try a diamond suspension that has more uniformly dispersed particles.

2) Is your sample mounted in epoxy? If so, perhaps particles are emedding in
the epoxy from a previous polishing step.

3) If your sample is not embedded in epoxy, perhaps the particles are embedded
in the mounting wax or trapped inside the polishing fixture.

I don't think your cloth would be a problem. What you are using is the same as
our Fine Rayon cloth and that is what I would recommend for what you are doing.
I assume that you are using a fresh cloth when you reach this stage. Of course,
oxidation could be the problem, but I have never had that problem so it doesn't
seem likely to me.

Something else you may want to consider is trying Colloidal Alumina for the
final polishing stage instead of Colloidal Silica. I understand that it can
produce a better finish on softer metals. Of course, I understand you are
having the problem before the colloidal silica, but it may be something to
consider when you get to that point.

Of course, I would be pleased to supply you with a sample of the diamond
suspension or the colloidal alumina if you would like to give that a try. I
would also be interested to hear any suggestions you get from other people and
ultimately your determination of the problem. I wish you good luck in figuring
it out and I encourage you to contact me if I can be of any help.

Best regards-

David Henriks
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673

TEL: 800-728-2233
FAX: 714-492-1499







From: GVKM07A-at-prodigy.com (DR CHARLES A GARBER)
Date: Thu, 06 Apr 1995 00:01:31 EDT
Subject: Diamond knives

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-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --


Further continuation of the diamond knife theme::

1] I have heard essentially good things about the Drukker company.
The only negatives I have ever heard came from Drukker competitors.

2] The Drukker people at the ICEM 94 in Paris and at MSA in 94 had in
their exhibit both a video demonstration showing some purported
advantages of their knife, because of the hydrophilic nature of the
knife, over other knives. The advantage supposedly was that the
sections come off more smoothly and with less vibration, e.g. slipping
and sliding down the edge of the blade. At the ICEM 94 in Paris, the
Drukker booth attracted some of the largest crowds.

Can anyone comment as to whether they have actually seen the
hydrophilic edge to be a unique advantage, above and beyond diamond
knives generally and therefore worth spending extra money?

Further with regard to Drukker, it is my understanding that they more
or less "own" the world wide business for diamond scalpel blades used
for radial keratotomy procedures being done by ophthamologists. They
are now trying to apply their expertise gained from the surgical
instruments end of the business to ultramicrotomy.

3] The "talk" seems to center around foreign made diamond knives.
There are some excellent US made knives (e.g. MicroStar and DDK) and
again, standing in our exhibit booths at the various meetings, I don't
detect any unhappiness among their customers either. As a group, they
do seem to be "happy campers". But as the US dollar stays weak
relative to European currencies, those campers are going to have
another reason to be "happy": They are going to be saving money.


Charles A. Garber
PRESIDENT
SPI SUPPLIES/STRUCTURE PROBE, INC.
PO BOX 656
West Chester, PA 19380 USA

Ph: (610) 436-5400
(800) 242-4774 [USA only]

e-mail: GVKM07A-at-prodigy.com






From: ychen-at-macc.wisc.edu
Date: Wed, 5 Apr 1995 11:59:43 -0600
Subject: Re: Bacteria cryosubstitution

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Message-Id: {25040512580307-at-vms2.macc.wisc.edu}


} Dear Cryo Electronmicroscopists,
} I have a student who wants to compare the quantity of extracellular
} polysaccharide material produced by bacterial cells grown on agar plates
} (we don't want to use cells from broth) by TEM. The bacteria are involved
} in plant nodule formation.
} We would like to loose as little of the material as possible during
} processing. I think plunge freezing with a KF80, cryosubstituting the cells
} using perhaps 2% OsO4 in methanol would be good, followed by conventional
} dehydration and embedding in epoxy. Conventional room temperature fix
} methods use glutaraldehyde/ruthenium red/OsO4 in various concentrations to
} fix and stain the extracellular material but from the pictures I've seen I
} suspect much of the material has still been lost.
}
} Is cryoprotection of the sample necessary? If I lightly fix then infiltrate
} in sucrose or some other protectant I'm a bit worried we'll start washing
} the polysaccharide away, if I don't then the cells (which will be a small
} scraping of colonies off the plate surface) could be ice damaged.
} If I don't use ruth. red as a stain will the material still be visible -
} even if present?
} I'd be grateful to hear from anyone who has had experiance of similar samples.
}
} Regards,
} Richard E
}
} Richard Easingwood
} South Campus Electron Microscope Unit
} Otago Medical School
} PO Box 913
} Dunedin
} NEW ZEALAND
}
} Telephone: 64-03-479 7301
} Facsimile: 64-03-479 7254
}
} "The southernmost electron microscope unit in the world"


You can try the method devoloped in Mueller's Lab, ETH, Switerland by using
a capillary tube.
"High-pressure freezing of cell suspensions in cellulose capillary tubes",
J. Microscopy 175, 34-43 (1994).






Ya Chen

=========================================================================
\ / Assistant Researcher-Cryo/SEM Coordinator TEL : 608-263-8481
\ / __ Integrated Microscopy Resource (IMR) FAX : 608-265-4076
\/ / / University of Wisconsin-Madison
/ / / 1675 Observatory Drive #167 Email:YChen-at-macc.wisc.edu
/ /__/_ Madison, WI 53706 Email:chen-at-calshp.cals.wisc.edu
=========================================================================






From: Veronique Buschmann :      bushman-at-ruca.ua.ac.be
Date: Thu, 6 Apr 1995 11:37:30 +0200 (METDST)
Subject: subscribe

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Please, subscribe : sci.techniques.microscopy
Thank you in advance.

-----------------------------------------------------------------
Veronique Buschmann email: bushman-at-ruca.ua.ac.be
EMAT phone: +32 3 218 02 46
University of Antwerp
-----------------------------------------------------------------





From: Tayloe :      tayloe-at-ptbma.usbm.gov
Date: Wed, 5 Apr 1995 14:13:09 -0400 (EDT)
Subject: Re: OM Polishing of Al

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Patrick,

I am not familiar with the Allied Spec-Cloth polishing cloths you are
using, nor have I polished 7075 Al (to my knowledge), but here is some
suggestions for ya to do with as ya see fit:

Using Buehler products, grind on grits 320, 400, 600, and 800;
polish on:
1. 6 micron diamond paste [water-based] on Texmet,
2. 1 micron diamond paste [water-based] on either Texmet or Metcloth,
3. either (a) 1 micron Cerium Oxide solution on Mastercloth -or-
(b) .5 micron Chrome Oxide solution on Mastercloth, then
4. .05 micron Mastermet [colloidal Silica] on Mastercloth.

Few hints:
- make sure new cloths are used, and that before their use, you "beat"
down the fibers on the Mastercloth by using a dummy/blank sample or such;
- if using a ultrasonic to help in cleaning, this may dislodge particles
that will then add scratches in later steps;
- use very warm water and -much- soap to clean the samples between each
step. I use my thumb to very gently rub and clean the sample surface;
- use of a dessicator is highly recommened to help slow oxidation and to
keep samples cleaner for further examination;
- takes much time and is -very- frustrating, but experiment with the
above and other recipes to get what works best for you;
- have not done much myself, but don't overlook electropolishing if such
can be used in your case;
- for me, water-based diamond pastes seem to "cut" better for rough
polishing, whereas oil-based seem to produce a smoother, cleaner finish;
- if get frustrated, quit if can for awhile, and attack the sample(s)
later on - have found myself that a recipe that works wonders one day
seems to suck later, but then seems to work great again! The joys of
hand-polishing!! oh boy...

Hope this is somewhat helpful. Good luck,
-Rob
disclaimer: [once again] am not affiliated nor have any financial
interest in any products mentioned or used
,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
| Rob Tayloe | MSM Spelunkers Club /\v/\ |
| Metallographic Lab. | Missouri Speleological Survey /\v/\ |
| Rolla Research Center | Bat Conservation International /\v/\ |
| U.S. Bureau of Mines | Missouri Cave & Karst Conservancy |
| tayloe-at-ptbma.usbm.gov | National Speleological Society #32993 /\v/\ |
| (314) 364-3169 x247 | American Cave Conservation Association |
''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''





From: bergrh-at-cc.memphis.edu (R. Howard Berg)
Date: Thu, 06 Apr 1995 08:04:31 +0600
Subject: Re: Bacteria cryosubstitution

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} You can try the method devoloped in Mueller's Lab, ETH, Switerland by using
} a capillary tube.
} "High-pressure freezing of cell suspensions in cellulose capillary tubes",
} J. Microscopy 175, 34-43 (1994).
}
} Ya Chen
}
Does anyone know of a US Supplier of dialysis tubing in the dimensions
specified in Mueller's paper (about the diameter of a capillary tube)?


R. Howard Berg, Ph.D.
Biology Department
University of Memphis
Memphis, TN, 38152
E mail: bergrh-at-cc.memphis.edu
phone: 901-678-4449 fax: 901-678-4457







From: MICROSCOPY-at-AAEM.AMC.ANL.GOV
Date: Fri, 7 Apr 1995 6:11:19 -0500 (CDT)
Subject: Newsgroups

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From: jfmjfm-at-umich.edu (John F. Mansfield)
Date: Thu, 06 Apr 1995 12:02:31 -0400
Subject: Re: ECHO Usenet

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Message-Id: {199504061559.LAA14193-at-srvr5.engin.umich.edu}

In article {3lsfh6$51e-at-newsbf02.news.aol.com} , campbell36-at-aol.com
(Campbell36) wrote:

} Is the Miscroscopy listserv still being echoed to this newgroup? I would
} appreciate any info regarding this or the usenet address and instructions
} on how to subscribe.
}
}
} Thanks...Jim Campbell

OK, here is the scoop.
The output of the Microscopy Mailserver at Argonne National Laboratory is
no longer being automatically forwarded to the Newsgroup
Sci.Techniques.Microscopy. The reason for this is that DEC closed down
the gateway I was using to forward the mail to the newsgroup. I do not
know where there is an alternative gateway and would like to know.
So, PLEASE, PLEASE, IF YOU KNOW HOW TO FORWARD A MAILING LIST TO A
NEWSGROUP THEN LET ME KNOW.

Also, if you know how to forward the newsgroup content to a mailing list
then please also let me know.
I know that we would have to parse the messages to prevent things being
sent in infinite loops, but it should be doable.

Please do not send messages to Nestor Zaluzec asking him how to do this,
he does know either!

Many thanks to whoever can help us.

--
John Mansfield
North Campus Electron Microbeam Analysis Laboratory
413 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (313)936-3352 FAX (313)936-3352
jfmjfm-at-engin.umich.edu or John.F.Mansfield-at-umich.edu
URL: http://www.engin.umich.edu/~jfmjfm/jfmjfm.html




From: MICROSCOPY-at-AAEM.AMC.ANL.GOV
Date: Fri, 7 Apr 1995 6:11:56 -0500 (CDT)
Subject: ESEM

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From: jfmjfm-at-umich.edu (John F. Mansfield)
Date: Thu, 06 Apr 1995 12:13:20 -0400
Subject: Environmental Scanning Electron Microscopy User Tips

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Message-Id: {199504061610.MAA15723-at-srvr5.engin.umich.edu}


Hi there,

At the Environmental Scanning Electron Microscopy Users Group Meeting in Monterey CA this last weekend, it was suggested that we keep a list of Tips, Hacks and Frequently Asked Questions about ESEM.
We have started to compile the list and I am soliciting input from the rest of the user community.
Please send anything you think might be applicable to me at the address below (email only please).

By The Way:
When we say ESEM people think mostly of the ElectroScan instrument. We are not talking exclusively about ElectroScan. We know that Topcon, Hitachi and JEOL (at the very least) make elevated pressure SEMs and all users of those instruments are encouraged
to reply too.

In fact since Hitachi calll their instruments "Variable Pressure SEMs", Topcon call theirs "Wet SEMs" and JEOL call theirs "Low Pressure SEMs", I propose that we call the generic instruments "Lousy Vacuum SEMs". Trouble is, LVSEM is JEOL's accronym! If
anyone has a better idea let me know.


--
John Mansfield
North Campus Electron Microbeam Analysis Laboratory
413 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (313)936-3352 FAX (313)936-3352
jfmjfm-at-engin.umich.edu or John.F.Mansfield-at-umich.edu
URL: http://www.engin.umich.edu/~jfmjfm/jfmjfm.html




From: MICROSCOPY-at-AAEM.AMC.ANL.GOV
Date: Fri, 7 Apr 1995 6:12:18 -0500 (CDT)
Subject: bacteria cryosubstitutio

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From: Doug Davis :      doug_davis-at-maillink.berkeley.edu
Date: 6 Apr 1995 09:53:27 -0700
Subject: Re: bacteria cryosubstitutio

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Message-ID: {n1414960321.97812-at-maillink.berkeley.edu}

Subject: Time: 8:01 AM
OFFICE MEMO RE} bacteria cryosubstitution Date: 4/6/95

to: Richard Easingwood
subj: cryosubstitution of bacteria

Richard- We have had some success with preserving the polysaccharide layer in
bacteria without the use of ruthenium red. A post-doc here custom-constructed
a growth chamber which allowed the growth of the bacteria on a filter
substrate. The idea was to examine the compaction of the bacteria under
different osmotic stresses without actually being immersed in the culture
media.
A small paper disc of cigarette paper was placed on the supporting filter
substrate and the bacteria were allowed to grow on top of it. The paper was
then removed and propane-jet frozen in an RMC MF 7200 device.
After a freeze-substitution regime using 1% osmium/0.1% UA in acetone, the
sample was allowed to slowly warm to RT, then embedded in Epon/Araldite 502
epoxy and sectioned on a diamond knife. Overall preservation was excellent.
I am aware of the preservative nature of RR on the polysaccharide layer but
we were surprised to see a great deal of extracellular matrix between the
cells without it. I am not sure what the mechanism of staining was in this
case, but I think it is significant to consider the possible mechanical loss
of the matrix due to processing/handling.
We have not repeated the experiment with the addition of RR to the
freeze-sub media so I do not yet have a comparison.

Regards,

Doug Davis
EML, 26 Giannini Hall
UC Berkeley 94720
(510) 642-2085
doug_davis-at-maillink.berkeley.edu





From: MICROSCOPY-at-AAEM.AMC.ANL.GOV
Date: Fri, 7 Apr 1995 6:16:20 -0500 (CDT)
Subject: T lymphocytes immunohistochemistry

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From: FRANCISCO J HERNANDEZ BLAZQUEZ :      fjhblazq-at-fox.cce.usp.br
Date: Fri, 7 Apr 1995 07:27:04 -0300 (BST)
Subject: T lymphocytes immunohistochemistry

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I'm studying lymphocytes in the bovine skin and I need some
information.

Please, does anyone have information about the identification of
T-lymphocytes subsets (T-helper and T-cytotoxic) in paraffin sections?
I mean, it is possible or it only can be done in cryostat sections.

I will be very grateful for any help.
=============================================================================
Francisco Javier Hernandez Blazquez |
Universidade de Sao Paulo - Faculdade de | e-mail fjhblazq-at-fox.cce.usp.br
Zootecnia e Engenharia de Alimentos | Voice: 55 195 616122 r. 265
Departamento de Ciencias Basicas/Histologia| r. 268
Av. Duque de Caxias Norte, 225 CP 23 | Fax: 55 195 611689
CEP 13630-000 Pirassununga (Sao Paulo) |
BRAZIL |
==============================================================================





From: ldm2-at-apollo.numis.nwu.edu (L.D.Marks)
Date: Fri, 7 Apr 1995 12:12:58 -0500
Subject: PC control of SEM

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I am interested in suggestions for a PC board to control an FEI
electron gun that is being used as a SEM.

Laurie Marks.




From: tivol-at-orkney.ph.albany.edu
Date: Fri, 07 Apr 1995 10:02:03 EDT
Subject: RE: Newsgroups

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Dear John,
I don't know how to forward mailservers to newsgroups or vice versa,
but I'll bet Dave Kristoferson (sp?), who moderates the bionet newsgroups,
does. Good luck; sorting this out will save a lot of people a lot of mailbox
room.
Yours,
Bill Tivol




From: BARBARA.HARTMAN-at-1773.220.SCHERING-PLOUGH.sprint.com
Date: Fri, 7 Apr 1995 14:50:52 -0400
Subject: VIRAL PARTICLE COUNTING

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GREETINGS,

I HAVE BEEN ASKED TO DO SOME VIRAL PARTICLE COUNTING. THE
INTERESTED PARTIES ARE CONCERNED ABOUT THE VIRAL CONCENTRATION FOR
CALIBTRATION OF OTHER ASSAYS SUCH AS 260/280 RATIOS AND HPLC.

I HAVE DONE SOME READING ON THE SUBJECT BUT WOULD APPRECIATE ANY
ADVICE ON WHICH TECHNIQUES ARE BETTER.

THANK YOU.


BARBARA HARTMAN
SCHERING-PLOUGH RESEARCH
LAFAYETTE, NJ
201-579-4343
201-579-4211 (FAX)
E-MAIL: BARBARA.HARTMAN-at-1773.220.SCHERING.PLOUGH.SPRINT.COM






From: MICROSCOPY-at-AAEM.AMC.ANL.GOV
Date: Fri, 7 Apr 1995 6:15:50 -0500 (CDT)
Subject: Re: Diamond knife discussion

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From: BARBARA.HARTMAN-at-1773.220.SCHERING-PLOUGH.sprint.com
Date: Thu, 6 Apr 1995 16:18:07 -0400
Subject: Diamond Knife Discussion

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X400-Received: by mta merit in /PRMD=internet/ADMD=telemail/C=us/; Relayed; Thu, 6 Apr 1995 17:52:52 -0400
X400-Received: by /ADMD=TELEMAIL/C=US/; Relayed; Thu, 6 Apr 1995 17:19:34 -0400
X400-Received: by /PRMD=SCHERING-PLOUGH/ADMD=TELEMAIL/C=US/; Relayed; Thu, 6 Apr 1995 16:18:07 -0400


IN REPLY TO ANTHONY GARRATT-REED, I DON'T DISAGREE WITH ALL OF YOUR
COMMENTS, BUT MARK ELLIOTT WAS ASKING FOR ADVICE FROM ANYBODY WHO HAD MORE
KNOWLEDGE THAN HIMSELF ON THE SUBJECT OF DIAMOND KNIFE PURCHASES. TRUE,
THE RESPONSES WERE OVERWHELMINGLY IN FAVOR OF ONE PARTICULAR COMPANY, BUT
ISN'T THAT THE INFORMATION THAT HE ASKED FOR? I WOULD PREFER TO PURCHASE A
KNIFE FROM A COMPANY THAT MANY PEOPLE THOUGHT CONSISTANTLY SELLS GOOD
QUALITY KNIVES AND HAS GREAT SERVICE THEN TO RISK BUYING A KNIFE FROM A
COMPANY THAT DIDN'T GET THE RAVE REVIEWS. I CONSIDER DIAMOND KNIVES AN
EXPENSIVE PURCHASE AND VALUE ANY WISDOM THAT MY CO-ELECTRON MICROSCOPISTS
HAVE TO OFFER.


BARBARA HARTMAN
SCHERING-PLOUGH RESEARCH
LAFAYETTE, NJ 07848
(201) 579-4343
(201) 579-4211 (FAX)
E-MAIL:BARBARA.HARTMAN-at-1773.220.SCHERING-PLOUGH.SPRINT.COM








From: Andreh Khachatouri :      usd16093-at-interramp.com
Date: Sat, 8 Apr 1995 01:22:38 -0800
Subject: SEM and TEM studies of Sea urchin

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X-Mailer: InterCon TCP/Connect II 2.1.2
Mime-Version: 1.0
Message-Id: {9504080122.AA38252-at-usd16093.interramp.com}

Hello.......
If anybody out there knows anything about SEM or TEM studies of surface
interactions between sea urchin eggs and beads( germ wheat, sugar beads) ,
please let me know. I will be very glad to know what fixative are you using
and what is the proportional amount of eggs compare to beads? thanks





From: kitajima-at-guarany.cpd.unb.br (Elliot W. Kitajima)
Date: Sat, 8 Apr 1995 07:36:41 -0200 (GRNLNDDT)
Subject: congress

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Dear colleagues of the AAEM: To whom should I contact so that I can place
informations regarding 3rd Interamerican Congress on Electron Microscopy to be
held from September 2 to 6, 1995, in Caxambu,MG, Brazil? Dr.Barbara Reine, from
Univ.Washington,Seattle,who belongs to AMS is the representantive from US in
the organizing committe. Would you plese contact me? Thanks. Kitajima.
--
Kitajima, Elliot Watanabe (kitajima-at-guarany.cpd.unb.br)
Departamento de Biologia Celular - Universidade de Brasilia
70919-970 - Brasilia - DF - Brasil
tel. +55-61-348-2424/+55-61-340-9094 fax +55-61-274-1065




From: MICROSCOPY-at-AAEM.AMC.ANL.GOV
Date: Fri, 7 Apr 1995 6:16:07 -0500 (CDT)
Subject: Re: OM Polishing of Al

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From: modum-at-gatan.com (Michael Odum)
Date: Thu, 6 Apr 1995 16:51:20 -0700
Subject: Re: OM Polishing of Al

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Message-Id: {199504062349.QAA22492-at-core.gatan.com}

Hello,

I have encountered your problem with fine scratches on aluminum and there
are a couple of things you might want to try. The first option is polish as
you have been, but skip the 1um and 0.25um diamond steps. I find that these
smaller particles tend to embed in the polishing cloth which act as your
scratch makers. And I recommend that you use 0.05 alumina suspension
instead of silica. I think silica is too agressive for fine polishing the
softer metals. If you feel this doesn't give you a fine enough finish you
can go to 0.03 alumina.

Option two is instead of diamond paste try cubic boron nitride. The
particles have rounded edges so they don't embed as easily. For soft metals
like Al I use 2-4um CBN then 0.05 alumina and I get a very good finish. I
get my CBN from Kay Industrial Diamond [1-800-327-8982]. Other than these
are the only people I know of who manufactures CBN pastes, for a resonable
price, I have no commercial interests.

In both options I try to keep actual polishing time to as little as I can
get away with, because the longer you polish you get a statistically better
chance of introducing some sort of scratch making contaminant. I hope this
was helpful.

Respectfully,

Michael W. Odum
Spec. Prep. Tech.
Gatan, Inc.
6678 Owens Dr.
Pleasanton, CA 94588
Tel: 510-463-0200
Fax: 510-463-0204






} We are currently trying to polish aluminum samples (7075). Before we etch
} them, we are trying to get a 0.05 micron finish. The grinding/polishing
} sequence is: 600 grit, 800 grit, 1200 grit, 3 micron diamond paste, 1 micron
} diamond paste, 0.25 micron paste, 0.05 colloidal silica. Everytime we go from
} the 1 micron step to the 0.25 micron step scratches appear....
}
} We have been using Allied High Tech Spec-Cloth polishing pads. Doeas anyone
} have suggestions to get to the final two steps without introducing scratches?
}
} I do not really understand why they get scratched so easily when the finer
} pastes are used.
}
} Should these samples be stored in a dessicator to prevent oxidation? I have
} never worked with Al, and therefore would appreciate any suggestions.
}
} Patrick Diehl
} Center for Materials Characterization
} University of North Texas





From: Jane A. Fagerland (708) 935-0104 :      FAGERLAND.JANE-at-igate.abbott.com
Date: Fri, 07 Apr 1995 09:33:00 -0600 (CST)
Subject: RE: T lymphocytes immunohistochemistry

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Mr-Received: by mta RANDB; Relayed; Fri, 07 Apr 1995 09:54:41 -0600
Mr-Received: by mta MCM$RAND; Relayed; Fri, 07 Apr 1995 09:54:46 -0600
Mr-Received: by mta RANDD; Relayed; Fri, 07 Apr 1995 09:55:02 -0600
Alternate-Recipient: prohibited
Disclose-Recipients: prohibited
Content-Return: prohibited

I tried to stain lymphocyte subsets in paraffin embedded sections of
bird lung fixed with a wide variety of fixatives - alcoholic, aldehydes,
PLP, imidates, etc. etc. - with no luck. I ended up using frozens.
However, I recently read a paper in which glycol methacrylate sections
were successfully stained for T cell subsets. The success was partly
due to using protease inhibitors in fixatives. I haven't tried this
method, but here is the reference:

Britten KM, Howarth PH, and Roche WR. 1993. Immunohistochemistry of
resin sections: a comparison of resin embedding techniques for small
mucosal biopsies. Biotechnic and Histochemistry 68(5):271-280.

The only other alternative is to use antibodies that recognize epitopes
after formalin fixation and paraffin embedding. I don't know if these
are available for bovine tissues. From past experience with veterinary
tissues, I'd be surprised if they were!

Good luck!

Jane A. Fagerland, Ph.D.
Dept. Cellular and Microscopic Research
Abbott Laboratories D45M/AP31
Abbot Park IL, 60064 USA






From: M.V. Parthasarathy :      mvp2-at-cornell.edu
Date: Sat, 8 Apr 1995 13:49:11 -0400 (EDT)
Subject: BA360 Free Fracture instrument spare part

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X-NUPop-Charset: English

Dear Fellow Microscopists,

I have been using an old Balzers BA360 freeze-fracture instrument for
teaching for the past several years. I neeed to replace the stainless-steel
bellows that is part of the LN2 plumbing for cooling the microtome knife.
Spare parts for BA360 is not available any more from Balzers/Bal-Tec. If you
have a BA360 that is scrapped and would like to sell or give away parts
including the bellows, kindly get in touch with me directly through e-mail.
Thanks!

*************************************************************************
M.V. Parthasarathy
Professor of Plant Biology & Director, EM Facility
Section of Plant Biology
228 Plant Science Building
Cornell University, Ithaca, NY 14850
Telephone: (607) 255-1734
Fax: (607) 255-5407
E-mail: mvp2-at-cornell.edu
***********************************************************************




From: Ann-Fook Yang :      YANGA-at-EM.AGR.CA
Date: Sat, 08 Apr 1995 11:59:43 -0400
Subject: Re:Polabed 812

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Message-Id: {sf867b14.017-at-EM.AGR.CA}
X-Mailer: Novell GroupWise 4.1

Someone was asking for information about POLABED 812.
I know of Polybed 812 from Polysciences.
In late 1970's Shell Chemicals decided to get out of Epon
production. They sold the stock to every distributor by ration,
just to be fair. I stocked up Epon myself and have some left. Two
years later, one company came up with its name prefix to "bed
812". Then many companies followed with their own xxxbed 812 to
replace Epon. The companies I dealt with recommended same formula
for mixing the components and claimed that it behaved like Epon.
Are these xxxbed 812 not the old Epon?

Ann Fook Yang







From: GVKM07A-at-prodigy.com (DR CHARLES A GARBER)
Date: Sat, 08 Apr 1995 20:54:06 EDT
Subject: Fwd: Re:Polabed 812

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Replying to posting of YANG, Ann-fook:

Your are correct in that there is some amount of selection of what are
sometimes called the "Epon 812 substitutes".

With regard to these substitutes, they are most definitely NOT all the
same. Our own SPI Pon 812 resin is indistinguishable from the original
Epon 812 when it was being manufactured. I make this point because
some of the Epon 812 that has been in the kind of long term storage
described, has in fact started to change, since the shelf life is not
unlimited, so if you were to compare one of the "substitutes"made today,
with what you have had in your storage, indeed you might find that
there are differences in the characteristics. Generally when the
changes are occurring they are undesirable and are likely to erode the
possibility of obtaining the best possible results.

Charles A. Garber
SPI Supplies
PO Box 656
West Chester, PA 19380 USA

Ph: 1-(610)-436-5400
1-(800)-242-4774

FAX:1-(610)-436-5755
e-mail: sp-supp-at-cerf.com
or
GVKM07A-at-prodigy.com
------- FORWARD, Original message follows -------

} Date: Saturday, 08-Apr-95 07:54 PM
}
} From: YANG, Ann-fook \ Internet: (yanga-at-em.agr.ca)
} To: Charles A. Garber, Ph. D. \ PRODIGY: (GVKM07A)
}
} Subject: Re:Polabed 812
}
} Someone was asking for information about POLABED 812.
} I know of Polybed 812 from Polysciences. In late 1970's Shell
Chemicals
} decided to get out of Epon production. They sold the stock to every
} distributor by ration, just to be fair. I stocked up Epon myself and
have
} some left. Two years later, one company came up with its name prefix
to
} "bed 812". Then many companies followed with their own xxxbed 812 to
} replace Epon. The companies I dealt with recommended same formula for
} mixing the components and claimed that it behaved like Epon. Are
these
} xxxbed 812 not the old Epon?
}
} Ann Fook Yang
}
}
}

------- FORWARD, End of original message -------







From: M.V. Parthasarathy :      mvp2-at-cornell.edu
Date: Sun, 9 Apr 1995 12:59:04 -0400 (EDT)
Subject: Environmental SEM and similar Instruments

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X-NUPop-Charset: English

Reply to J.H. Mansfield's comments on ESEM type SEMs:

In addition to the ESEM, the Variable Pressure SEM of Hitachi and the Low
Vacuum SEM of Jeol, there is now the new ECO-SEM (Environment controlled
Sanning EM) introduced by AMRAY at Pittcon '95. How about coining the acronym
SAPSEM--SubAtmospheric Pressure Scanning Electron Microscope--when referring
to this class of SEMs?

*************************************************************************
M.V. Parthasarathy
Professor of Plant Biology & Director, EM Facility
Section of Plant Biology
228 Plant Science Building
Cornell University, Ithaca, NY 14850
Telephone: (607) 255-1734
Fax: (607) 255-5407
E-mail: mvp2-at-cornell.edu
***********************************************************************




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Tue, 11 Apr 1995 11:53:22 -0500 (CDT)
Subject: Looping Messages...

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G'day Subscribers...

I returned from the MSA Program Production Meeting
this morning and found the Listserver looping a series
of messages. I believe I have found the source and
have temporairly shutdown the list until I clear up
the mess (and also delete the addresses causing the problems).
In the interim, we may loose a few postings. Hopefully
they will be retrievable. Please wait until you receive
a back on-line message from me before you repost your
messages to the list.

Sorry, but as Murphy has it, the problem ocurred while I was out
of town for a few days.

We should be back on-line later tonight.

Nestor





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Tue, 11 Apr 1995 20:52:41 -0500 (CDT)
Subject: Analysis of the Bouncing Message--Why

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Ev'ning Subscribers.....

Well I've sorted out what I think is the source
of the bounce. If your curious read on. Otherwise
trash this. I'm going to leave the node down for
the rest of the evening just to try make sure all of
the bouncing/reflections have finished.

----------------------------------------------------
Here's what happened..

1.) A user turned on an automatic reply in his Email
program. This automatic reply sent the message
"Thank you for Remembering...."
back to anyone that sent him a message.
2.) When a message that originated at the Microscopy list
was sent to this user, his program sent a reply
back to the list, which (by design) then sent
this message out to everyone on the list. This
of course included the user with the automatic
reply. The automatic reply then kicked in (again)
and the cycle started the FIRST infinite loop.
3.)After many of these cycles, a different user in
Australia had their mailbox fillup. Their program
next generated an error message which was sent back
to the list, which went to everyone, including
our "Thank-you" user. This started a SECOND infinite
loop, since now everytime the message went out their
mailbox was still full and the error message was
sent again.
4.)At least 2 more users (one in Hungary and another in Slovenia)
had their mailboxes fill....
5.)The cycle just gets worse from there.

What made matters worse is that I was out of town from Friday AM, through
Tuesday AM. Although I did a quick check on Friday PM I did not see
anything that was grossly out of line, but by Tuesday AM there was
a queue of some 100,000+ messages waiting to be delivered!

I had no intention of trying to sort throught that lot to see which
were real or which were the echo so I shut down the whole system
and started trashing things.

We will restart probably tomorrow, when I'm pretty sure that
things have cleared up.

I've managed to save a some of the last few posting. I'll
try to resend them as tests.

G'night All.... Nestor





From: ANLAEM::MICROSCOPY 11-APR-1995 11:33:56.78
Date: Wed, 12 Apr 1995 14:00:31 -0500 (CDT)
Subject: Be Window replacement

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From: KAKER-at-ctklj.ctk.si
Date: Mon, 10 Apr 1995 10:38:14 +0200 (WET-DST)
Subject: Window Mount

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In our laboratory we have a old Edax 9100 detector with Be window.
In future we wish replace this Be window with Moxtek window. I'm
looking information, how is window mount mechanicaly mounted to the
end cup. Has anyone had experience with replacing Be window in the
EDS detector. Thank you.

Henrik Kaker
kaker-at-ctklj.ctk.si




From: ANLAEM::MICROSCOPY 11-APR-1995 11:37:46.11
Date: Wed, 12 Apr 1995 14:03:17 -0500 (CDT)
Subject: Capillatory tubes for Bacteria cryosubstitution

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From: ANLAEM::MICROSCOPY 11-APR-1995 11:38:14.82
Date: Wed, 12 Apr 1995 14:04:22 -0500 (CDT)
Subject: OsO4 'oxidation'

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Message-ID: {BC5D8A2F0179AEAA-at-ggpl.arsusda.gov}





From: Richard E. Edelmann :      EDELMARE-at-CASMAIL.MUOHIO.EDU
Date: Tue, 11 Apr 1995 12:01:56 -500
Subject: OsO4 'oxidation'

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Just a general chemistry question on OsO4 degradation. When OsO4
breaks down in aqueous solution and turns brown, we generally consider
it no good. And quite often it is refered to as having been
"oxidized'. But if you expose Osmium metal to ambient air it will
oxidize to form OsO4 (Merck Index). I am just currious does anybody
know what happens to the aqueous OsO4 when it turns brown?


Richard E. Edelmann
Electron Microscopy Facility Supervisor
352 Biological Science Building
Miami University, Oxford, OH 45056
Ph: 513-529-5712
E-mail: edelmare-at-muohio.edu




From: ANLAEM::MICROSCOPY 11-APR-1995 11:35:18.61
Date: Wed, 12 Apr 1995 14:00:56 -0500 (CDT)
Subject: Nitrogen in EDS

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From: Leszek Kepinski :      kepinski-at-highscreen.int.pan.wroc.pl
Date: Tue, 11 Apr 1995 11:22:52 +0100 (CET)
Subject: Nitrogen in EDS

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X-NUPop-Charset: English

As a non specialist in EDS I have a question.
Is it possible that our EDS detector (UTW from EDAX) attached to SEM does not
"see" nitrogen peak at 0.91 keV (even in AlN and GaN it is hardly visible),
though at the same time it detects easily very close C and O peaks?
Leszek Kepinski


*****************************************************************
Leszek Kepinski
Institute of Low Temperature and Structure Research,
Polish Academy of Sciences, P.O.Box 937, 50-950 Wroclaw, Poland
tel:(4871) 350 21 ext.153
fax:(4871) 44 10 29
email: kepinski-at-highscreen.int.pan.wroc.pl
*****************************************************************




From: ANLAEM::MICROSCOPY 11-APR-1995 11:35:33.52
Date: Wed, 12 Apr 1995 14:01:26 -0500 (CDT)
Subject: 5th Intl Conference on Image Processing & its Applications

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From: lporter-at-goodyear.com (LE Porter)
Date: Tue, 11 Apr 1995 09:05:29 -0400
Subject: 5th Intl Conference on Image Processing & its Applications

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Message-Id: {9504111300.AA02749-at-rds163.noname}
X-Sender: t456b19-at-rds163
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"


Dear Imagers,

Does anyone out there have anything more specific on the following: 5th
Intl Conference on Image Processing & its Applications, Details: S
Griffiths, IEE, Savoy Place, London WC2R OBL? I am looking for an email
address, tentative proceedings, etc.
Thanks for your help

L E Porter Phone (216) 796-1620
Head of Microscopy Fax (216) 796-3304
The Goodyear Tire & Rubber Company EMail LPORTER-at-GOODYEAR.COM
Dept 415A
142 Goodyear Blvd
Akron, OH 44305
USA







From: ANLAEM::MICROSCOPY 12-APR-1995 08:47:16.57
Date: Wed, 12 Apr 1995 14:02:38 -0500 (CDT)
Subject: Correction on N peak

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From: Leszek Kepinski :      kepinski-at-highscreen.int.pan.wroc.pl
Date: Wed, 12 Apr 1995 08:23:09 +0100 (CET)
Subject: Re: Nitrogen in EDS

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X-NUPop-Charset: English

{Date: Tue, 11 Apr 1995 11:22:52 +0100 (CET)
{From: "Leszek Kepinski" {kepinski-at-156.17.85.7}
{To: MICROSCOPY-at-AAEM.AMC.ANL.GOV

{As a non specialist in EDS I have a question.
{Is it possible that our EDS detector (UTW from EDAX) attached to SEM does not
{"see" nitrogen peak at 0.91 keV (even in AlN and GaN it is hardly visible),
{though at the same time it detects easily very close C and O peaks?

{Leszek Kepinski kepinski-at-highscreen.int.pan.wroc.pl

Sorry for the mistake. The considered nitrogen peak is of course at 0.39
keV. As you see I am really non-specialist.

Leszek Kepinski


*****************************************************************
Leszek Kepinski
Institute of Low Temperature and Structure Research,
Polish Academy of Sciences, P.O.Box 937, 50-950 Wroclaw, Poland
tel:(4871) 350 21 ext.153
fax:(4871) 44 10 29
email: kepinski-at-highscreen.int.pan.wroc.pl
*****************************************************************




From: ANLAEM::MICROSCOPY 11-APR-1995 11:38:00.19
Date: Wed, 12 Apr 1995 14:03:59 -0500 (CDT)
Subject: gluing sapphire

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From: Georges_Veilleux-at-INRS-ENER.UQuebec.CA (Georges Veilleux)
Date: Tue, 11 Apr 1995 11:39:24 -0400
Subject: gluing sapphire

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Does anybody know a method of mounting sapphire to make cross-
sections for TEM ?

We have used the GATAN G1 EPOXY glue whitout success.

We know a lot of methods to prepare cross-section specimens. We are
able to prepare samples in plane without difficulty and cross-sections with
different materials but sapphire.

It seems it would be the adhesion of the glue on sapphire which is the
problem with our cross-section samples. Any idea on the type of glue that
should be used.

Thank you.
Georges Veilleux
Researcher
INRS-Energie et materiaux
C.P. 1020
Varennes, Qc
Canada
J3X 1S2
Tel.: (514) 929-8110
Fax: (514) 929-8102





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Thu, 13 Apr 1995 9:37:58 -0500 (CDT)
Subject: Meetings/Conferences

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Subscribers...

The Listserver is up and running, but I will
for the short term be checking/monitoring each contribution
for a reflection/bounce before I turn it back into
a free-for-all system as it was in the past. I'll not moderate, just
intercept all messages and look at them first
before passing them along. My guess is that
in another day all the bounces will have finally
cleared up. I saw a few from last week late last night.
Because of this checking (manual) postings will appear
abit behind what your used to, as I will be doing it
late in the evening..


I've also had multiple requests for information about
meeting schedules. I've now added a section to
the Microscopy&Microanalysis WWW site which has
dates/locations/contacts etc.. for different
meetings that I know about. As I receive updates
or see items of interest I will update the lists.

You may access this information at:

http://www.amc.anl.gov


Your Friendly (& lately blurry eyed) Neighborhood SysOp

Nestor





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Thu, 13 Apr 1995 9:59:31 -0500 (CDT)
Subject: Window Mount

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From: _ _ _ _ _ MARK W. LUND _ _ _ _ _ :      LUNDM-at-PHYSC1.BYU.EDU
Date: Thu, 13 Apr 1995 09:04 MDT
Subject: Window Mount

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The window mount is attached to the detector snout with epoxy.
I should point out that it is difficult to predict the results of
putting a light-element window on an old detector. You do not know
the dead layer (or what is sometimes called the "detector window") thickness,
or the attenuation from the front electrode. I also would like to hear
of any experiences others have had doing this.
regards
mark

Mark W. Lund, Ph
Director
MOXTEK, Inc.
Orem UT




From: ANLAEM::MICROSCOPY 12-APR-1995 08:48:40.09
Date: Thu, 13 Apr 1995 10:15:01 -0500 (CDT)
Subject: MPSEM/ESEM

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From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Wed, 12 Apr 1995 8:42:39 -0500 (CDT)
Subject: MPSEM/ESEM

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From: Alan Pooley :      pooley-at-ahab.rutgers.edu
Date: Wed, 12 Apr 1995 08:42:28 -0400
Subject: Re: Undeliverable Mail

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how about mid-pressure SEM ie MPSEM pronounced empsem

just a thought from a lpsem user.....
alan pooley




From: ANLAEM::MICROSCOPY 12-APR-1995 08:48:23.42
Date: Thu, 13 Apr 1995 10:14:27 -0500 (CDT)
Subject: Newsgroups

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From: Marcelle A Gillott :      magem-at-csd.uwm.edu
Date: Wed, 12 Apr 1995 09:05:48 -0500 (CDT)
Subject: Re: Newsgroups

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I am now sure that it weould be a good idea to forward the newsgroup
stuff to the listserver........
the messages on this mail list outomatically get sent to all subscribers,
many of whom are on commercial links and therefore have to pay by the message

Although this would not affect me cost-wise, it would vastly increase my
volume & I am unsure about whether I would benefit from that -

Please make a concerted effot to check out all the ramifications of
such an action before doing something that may change the character of
this activity and try to get a concensus as to its desirability

thanks

marcelle gillott
uwm

ps - I am not trying to be a spoilsport - more like a devil's advocate!






From: ANLAEM::MICROSCOPY 11-APR-1995 11:36:36.51
Date: Wed, 12 Apr 1995 14:01:54 -0500 (CDT)
Subject: W-filament discussion

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From: xxie-at-lsuvm.sncc.lsu.edu (Xiaogang Xie)
Date: Mon, 10 Apr 1995 09:35:58 -0600
Subject: Summary about "W filament advises"

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Summary about "W Filament Advises" discusses

Ten days ago, I posted a message to ask advises about (a). new W
filament vs. rebuilt one; (b). better sources to order filament; (c). a
problem about a bad batch of filaments. A few people provided their
experiences and more people asked for a summary. THANKS FOR ALL OF YOU!

1. New W filament vs. Rebuilt one
(a). Price: A box of 12-piece JOEL K-type filament is about
$500-$600 for the new ones from manufacturer, and $120-$180 for the rebuilt
ones.
(b).Pros: three people believe that the rebuilt one is as good as
the new one, and two of them have better experience with the rebuilt one.
(c). Cons: however, I also got "boo" about the rebuilt one from two
people. One of them pointed out that W hairpin bend and distortion is a
common problem with the rebuilt one.

2. Better vendor source for W filament
No conclusion. Five different sources are mentioned in these
response messages. Most people do not have preference in any specific
vendor except two of them told me that they enjoy SPI and one prefers EBTEC
for rebuilt filament.

3. Other tips
A suggestion for achieving better filament performance that I got
is to preheat the filament before mounting it.

Xiaogang

****************************************
* Xiaogang Xie *
* Department of Geology and Geophysics *
* Louisiana State University *
* Baton Rouge, LA 70803 *
* Office (504)388-2240 *
* Fax (504)388-2302 *
****************************************






From: ANLAEM::MICROSCOPY 11-APR-1995 11:37:30.31
Date: Wed, 12 Apr 1995 14:02:10 -0500 (CDT)
Subject: EDXA Book

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From: Laurie Frederick :      frederick_laurie-at-vanlab.paprican.ca
Date: Mon, 10 Apr 1995 16:53:29 PDT
Subject: Re: EDXA Book

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Message-Id: {MAILQUEUE-101.950410165329.480-at-vanlab.paprican.ca}
To: "DAVID VOWLES" {VOWLES-at-rsbs-central.anu.ed

Dear David,
I received a very helpful responce from Dave Williams, and yes the
(his?) book has been published. It is available from Plenum Press, NY.
Their phone number is (212) 620-8000. It's ISBN 0306-448-580 and lists
for $79.50 US.
I'm looking forward to it.
Laurie

} Dear Laurie,
}
} Recently you posted an enquiry to the Microscopy Listserver:
} "Has the book from MAS entitled "X-ray Spectrometry in Electron
} Beam Instruments" by Dave Williams and Dale Newbury actually
} been published yet and if so, is there a phone number for orders?"
}
} Did you receive any answer to this question? I am interested in
} obtaining a copy of the above book by Williams and Newbury and
} would be grateful if you could forward any details which you may
} have.
}
} With thanks,
}
} David Vowles
} Electron Microscopy Unit
} Australian National University
} Email: David.Vowles-at-anu.edu.au
}

______________________________________________________________________
Laurie Frederick, A.SC.T. PAPRICAN
Corrosion Control Group 3800 Wesbrook Mall
The Pulp and Paper Research Vancouver, B.C.
Institute of Canada Canada V6S 2L9

Email: frederick_laurie-at-vanlab.paprican.ca
Tel: 604-222-3200 Fax: 604-222-3207




From: DLIETZ-at-TrentU.ca
Date: Thu, 13 Apr 1995 14:55:39 -0400 (EDT)
Subject: position available

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Dear Colleges
There is a full-time contract position in Electron Microscopy
coming up at Trent University which is located in Peterborough Ontario.
The position is available from May 15 to December 22 1995 and is in the dept.
of biology. Applicants interested please reply and a job description can
be faxed to you.


SEND ALL REQUESTS to: DLIETZ-at-TrentU.ca






From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Fri, 14 Apr 1995 8:43:49 -0500 (CDT)
Subject: Freeze-sub w/Ruthenium Red

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From: Doug Davis :      doug_davis-at-maillink.berkeley.edu
Date: 13 Apr 1995 12:03:05 -0700
Subject: Freeze-sub w/Ruthenium Red

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Message-ID: {n1414347570.62701-at-maillink.berkeley.edu}

Subject: Time: 10:59 AM
OFFICE MEMO Freeze-sub w/Ruthenium Red Date: 4/13/95


Anyone have any experience with Ruthenium Red in freeze-substitution? Seems
to me that RR is not soluble in 100% acetone.
Thanks....
doug_davis-at-maillink.berkeley.edu









From: DLIETZ-at-TrentU.ca
Date: Thu, 13 Apr 1995 15:06:11 -0400 (EDT)
Subject: books for sale

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Recently, I had seen a book entitled "MICROCOSMOS" BY Jeremy Burgess ,
Michael Marten and Rosemary Taylor . The book is now out of print and I'm
interested in purchasing a second hand book. I work at Trent University
and often do high school tours for the area. I thought this type of book
would peek the interests of those students as well as lower year
university students into the field of microscopy. If anyone would like to
part with their copy or know where I can purchase a copy please reply. Or
if there is a title of a more recent edition to the book recently in print
please forward .
THANKS DEBBIE





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Fri, 14 Apr 1995 8:50:52 -0500 (CDT)
Subject: 5th Intl Conference on Image Processing & its Applications

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From: ANLAEM::MICROSCOPY 11-APR-1995 11:35:33.52
Date: Wed, 12 Apr 1995 14:01:26 -0500 (CDT)
Subject: 5th Intl Conference on Image Processing & its Applications

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From: lporter-at-goodyear.com (LE Porter)
Date: Tue, 11 Apr 1995 09:05:29 -0400
Subject: 5th Intl Conference on Image Processing & its Applications

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Message-Id: {9504111300.AA02749-at-rds163.noname}
X-Sender: t456b19-at-rds163
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"


Dear Imagers,

Does anyone out there have anything more specific on the following: 5th
Intl Conference on Image Processing & its Applications, Details: S
Griffiths, IEE, Savoy Place, London WC2R OBL? I am looking for an email
address, tentative proceedings, etc.
Thanks for your help

L E Porter Phone (216) 796-1620
Head of Microscopy Fax (216) 796-3304
The Goodyear Tire & Rubber Company EMail LPORTER-at-GOODYEAR.COM
Dept 415A
142 Goodyear Blvd
Akron, OH 44305
USA







From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Fri, 14 Apr 1995 15:55:50 -0500 (CDT)
Subject: Residual Gas Analyzer: req for info

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From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Fri, 14 Apr 1995 15:56:56 -0500 (CDT)
Subject: SGI-SEM Interface

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From: Greg Erdos ICBR EM Core Lab Univers :      GWERDOS-at-gnv.ifas.ufl.edu
Date: Thu, 13 Apr 1995 13:16:17 -0500 (EST)
Subject: SGI - SEM interface

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Is there nayone out there who is using a UNIX based system, SGI Indy in
particular to grab images from a Hitachi S-4000 or later FESEM. I would like
to know how you configured your system to do this, e.g., do you get the digital
signal or tv output? do you you go directly to the SGI video in or do you have
something between etc.

Thanks much
******************************************************
* Greg Erdos ** *
* Director, ICBR EMCL ** Phone 904-392-1295 *
* 218 Carr Hall ** FAX 904-846-0251 *
* University of Florida ** gwerdos-at-gnv.ifas.ufl.edu *
* Gainesville, FL 32611 ** *
******************************************************************




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Fri, 14 Apr 1995 15:57:39 -0500 (CDT)
Subject: Cy% recommendations needed

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From: almonte-at-medcolpa.edu
Date: Wed, 12 Apr 1995 16:19:30 -0400
Subject: Cy5 problem

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I'd like some recommendation on what kind of filter blocks and lines to use
to view Cy5. I'm using a MRC 600. I haven't been able to view any
fluorescence with Cy5 using filter block "RHS" line 647 DF10, Neutral
Density set at 1.
I have tried different dilutions: 1:100, 1:200, 1:400, 1:600 for Cy5 1.4
mg/ml. None of these dilutions have given me good results. Any
recommendation would be appreciated.
Thanks in advance,
Ciprian

__________________________________________________________
Ciprian Almonte * * :)
*
Medical College of Pennsylvania
Dept. of Anatomy and Neurobiology * * *

3200 Henry Ave. :)
Philadelphia, PA 19129
E-mail: almonte-at-medcolpa.edu * * *
Voice: (215) 842-4081
Fax: (215) 843-9082 *
* :)
__________________________________________________________







From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Fri, 14 Apr 1995 16:05:22 -0500 (CDT)
Subject: Looking for a old book

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From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Fri, 14 Apr 1995 15:58:23 -0500 (CDT)
Subject: OsO4 Oxidation

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From: ANLAEM::MICROSCOPY 11-APR-1995 11:38:14.82
Date: Wednesday, April 12, 1995 2:04PM
Subject: OsO4 'oxidation'

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From: Richard E. Edelmann :      EDELMARE-at-CASMAIL.MUOHIO.EDU
Date: Tue, 11 Apr 1995 12:01:56 -500
Subject: OsO4 'oxidation'

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Just a general chemistry question on OsO4 degradation. When OsO4
breaks down in aqueous solution and turns brown, we generally consider
it no good. And quite often it is refered to as having been
"oxidized'. But if you expose Osmium metal to ambient air it will
oxidize to form OsO4 (Merck Index). I am just currious does anybody
know what happens to the aqueous OsO4 when it turns brown?


Richard E. Edelmann
Electron Microscopy Facility Supervisor
352 Biological Science Building
Miami University, Oxford, OH 45056
Ph: 513-529-5712
E-mail: edelmare-at-muohio.edu




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Fri, 14 Apr 1995 20:44:18 -0500 (CDT)
Subject: reply/Nitrogen in EDS

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From: ANLAEM::MICROSCOPY 11-APR-1995 11:35:18.61
Date: Thu, 13 Apr 1995 13:00 -0700 (PDT)
Subject: Re: Nitrogen in EDS

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From: Leszek Kepinski :      kepinski-at-highscreen.int.pan.wroc.pl
Date: Tue, 11 Apr 1995 11:22:52 +0100 (CET)
Subject: Nitrogen in EDS

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X-NUPop-Charset: English

As a non specialist in EDS I have a question.
Is it possible that our EDS detector (UTW from EDAX) attached to SEM does not
"see" nitrogen peak at 0.91 keV (even in AlN and GaN it is hardly visible),
though at the same time it detects easily very close C and O peaks?
Leszek Kepinski


*****************************************************************
Leszek Kepinski
Institute of Low Temperature and Structure Research,
Polish Academy of Sciences, P.O.Box 937, 50-950 Wroclaw, Poland
tel:(4871) 350 21 ext.153
fax:(4871) 44 10 29
email: kepinski-at-highscreen.int.pan.wroc.pl
*****************************************************************


It's not unusual to have relatively poor sensitivity for nitrogen in UTW or ATW
detectors. Nitrogen x-rays are strongly absorbed by carbon that may be present
on the sample surface(s), on the detector window, or--in some detectors--as a
detector window material. Carbon and oxygen x-rays are less strongly absorbed
by carbon films. You could also get high differential absorption of nitrogen x-
rays by specific elements in bulk samples, but not by Al or Ga.

What can you do about it?
1. To enhance the detection of light elements in a SEM, try operating at low KV
(~2-5 KV) to increase the yield of soft x-rays relative to that of higher
energy x-rays. Increase the beam spot size to obtain the beam current needed
to maintain an optimum counting rate.

2. Be sure the sample surface is clean. (A 10-20 nm thick anticharge coating
of carbon on the sample won't affect the soft x-ray absorption significantly).

3. If the UTW window is dirty, it may require replacement (a factory job in
most cases, but can be done by the user if the detector has a turret-type
window assembly.

4. If the detector crystal is dirty, contact the manufacturer about cleaning
or replacing it (usually very expensive).

5. For an SEM with a high rate of hydrocarbon contamination (also not
unusual), consider one of the hydrocarbon cleaning systems now on the market
(or make your own).

6. Probably most important: Try calculating what the x-ray spectra from your
detector should look like, to find out what the nitrogen detectability should
be for different experimental conditions. The DTSA program for Macintosh
computers, from NIH/NIST, performs this function.

Larry Thomas
Battelle, PNL
Richland, WA, USA




From: kvecchio-at-ames.UCSD.EDU
Date: Fri, 14 Apr 1995 20:47:41 -0500 (CDT)
Subject: Opening for Microscopist Post-Doc

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Dear Collegues:

This message is on behalf of Professor Nemat-Nasser in the Applied
Mechanics and Engineering Sciences Department at the University of CA, San
Diego. Prof. Nemat-Nasser has an opening for a post-doctoral researcher
with expertise in electron microscopy of deformation mechanisms and damage
evolution. The candidate would be working closely with researchers in the
area of mechanics of materials, and is expected to collaborate of several
projects involving deformation and fracture of both ductile metals and
brittle solids. Some of this research will be focused on understanding
deformation behaviors at high strain rates, an area where there is
currently considerable research in our group here at UCSD.

Interested candidates should sent a resume, list of publications, and three
references directly to Professor Nemat-Nasser -at- Dept. of AMES, MC-0411,
Univ. of CA, San Diego, La Jolla, CA 92093.

E-mail files may be send to: Sia-at-CEAM.UCSD.EDU, but should be followed up
with a hard copy sent by regular mail. The position will be open until an
suitable candidate is found to fill the position.

Please do not send any responses to the author of this message, but direct
all future correspondences to Prof. Nemat-Nasser.







From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Fri, 14 Apr 1995 20:49:00 -0500 (CDT)
Subject: Nitrogen in EDS

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From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Fri, 14 Apr 1995 20:49:00 -0500 (CDT)
Subject: Nitrogen in EDS

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} email: kepinski-at-highscreen.int.pan.wroc.pl
} *****************************************************************
}
} Yes, it is quite possible that your window has poor Nitrogen transmission.
For instance, Noran used to market a window material called ZMAX ( a diamond
film?). ZMAX had very poor Nitrogen transmission. You should press EDAX
for more information regarding your Window type and transmission properties.

Paul Thomson





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Fri, 14 Apr 1995 20:52:26 -0500 (CDT)
Subject: Old TEM Available

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From: Marcelle A Gillott :      magem-at-csd.uwm.edu
Date: Fri, 14 Apr 1995 09:22:40 -0500 (CDT)
Subject: Old TEM available

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Anyone interested in an Hitachi HU 11B ???

I have one that is still under vacuum (was last used about 2 years ago
for a demonstration) - we have lots of extra parts, including some
specialized stages that were obtained when someone else's 11B was
decommissioned. ... The space it is currently occupying is now needed
for another activity -

Please contact me directly if you are interested -

Marcelle Gillott
University of Wisconsin-Milwaukee





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Fri, 14 Apr 1995 20:56:16 -0500 (CDT)
Subject: Viral Particle Counting

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From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Fri, 14 Apr 1995 20:56:16 -0500 (CDT)
Subject: Viral Particle Counting

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GREETINGS,

I POSTED THIS QUESTION LAST WEEK AND DID'T GET A RESPONSE SO I
THOUGHT I'D GIVE IT ANOTHER TRY.

I HAVE BEEN ASKED TO DO SOME VIRAL PARTICLE COUNTING BY TEM. THE
INTERESTED PARTIES ARE CONCERNED ABOUT THE VIRAL CONCENTRATION FOR
CALIBRATION OF OTHER ASSAYS SUCH AS 260/280 RATIOS AND HPLC.

I HAVE DONE SOME READING ON THE SUBJECT BUT WOULD APPRECIATE ANY
ADVICE ON TECHNIQUES.


THANK YOU.


BARBARA HARTMAN
SCHERING-PLOUGH RESEARCH
LAFAYETTE, NJ
201-579-4343
201-579-4211 (FAX)
E-MAIL: BARBARA.HARTMAN-at-1773.220.SCHERING.PLOUGH.SPRINT.COM






From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Fri, 14 Apr 1995 20:59:51 -0500 (CDT)
Subject: Fatty Acid Stain

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From: DIRK DOMASCHKO :      DOMASCHK-at-musom01.mu.wvnet.edu
Date: Fri, 14 Apr 1995 13:33:21 +1100
Subject: Fatty Acid Stain

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Does anyone out there know of a good method for stainning fatty acids
for TEM? Both intra and extracellular fatty acids are of intrest in
red skeletal muscle, if it makes any difference in the procedure.

Thanks in Advance for any help.










Dirk W. Domaschko
Electron Microscopy Facility
Marshall University School of Medicine
Huntington, WV 25755-9350
Ph: 304-696-7393
E-Mail: Domaschk-at-musom01.mu.wvnet.edu




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Fri, 14 Apr 1995 21:01:28 -0500 (CDT)
Subject: Re: Freeze-sub w/Ruthenium Red

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From: tphillips-at-biosci.mbp.missouri.edu (Tom Phillips)
Date: Fri, 14 Apr 1995 13:07:16 -0500
Subject: Re: Freeze-sub w/Ruthenium Red

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how about tetrahydrofuran. it is a great freeze sub alternative to acetone.

Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility
3 Tucker Hall
University of Missouri
Columbia, MO 65211
(314)-882-4712 (voice)
(314)-882-0123 (fax)






From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Fri, 14 Apr 1995 20:49:53 -0500 (CDT)
Subject: Gluing Sapphire

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From: tsi-at-werple.mira.net.au (Thomson Scientific:Paul Thomson)
Date: Fri, 14 Apr 1995 12:50:05 +1000
Subject: Re: gluing sapphire

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} From: ANLAEM::MICROSCOPY 11-APR-1995 11:38:00.19
} To: ZALUZEC
} CC:
} Subj: gluing sapphire
}



From: tsi-at-werple.mira.net.au (Thomson Scientific:Paul Thomson)
Date: Fri, 14 Apr 1995 12:50:05 +1000
Subject: Re: gluing sapphire

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Paul Thomson





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Fri, 14 Apr 1995 20:51:14 -0500 (CDT)
Subject: OsO4 Oxidation

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From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Fri, 14 Apr 1995 20:51:14 -0500 (CDT)
Subject: OsO4 Oxidation

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I would like to respond as follows:

The valence state of Os in the tetroxide is presumed to be +8.

As the valence state is reduced to lower valence states there is a
whole series of color changes, and by the time it gets to +2, the color
is black.

When one has a vial that has started to turn brown, more likely than
not, it is not a unique valence state but a mixture of valence states,
or some purists might say a distribution of valence states and
continues to become more and more black in color as the extent of
reduction proceeds further.

At that point, in the ampoule, the change is irreversible and there is
nothing anyone can do to reverse that change (without breaking open the
ampoule) and doing actual chemistry to reverse the reactions..

Here are some options to discarding the spoiled (for some applications)
ampoules::

1] Some people could still make use of the aqueous osmium , for
example, those doing vapor phase staining of rubber modified polymers.
For such work, it does not really matter that much whether the solution
is "100% active" or not. It is the vapors that count. This might not
be true for everyone but at least it is one reason why you need not
automatically think of sending it off to some kind of hazardous waste
container. I would imagine there would be student uses for the "brown"
osmium product as well.

2] If you absolutely have no use for it and if you have some quantity
of ampoules, the osmium can be recycled. Or even if you don't have a
whole lot, we can still combine it with other ampoules and recycle the
reduced osmium. Contact me by e-mail if you wanted to explore that
option. Osmium is a non-renewable resource and we should try to
preserve it. The osmium you would send back might not have any real
economic value in the sense that you would get anything back for it,
but it is usually a lower cost alternative relative to the cost of
disposing of it as a hazardous waste.

Charles A. Garber
SPI Supplies
PO Box 656
West Chester, PA 19380

Ph: 1-(610) 436-5400 FAX:1-(610) 436-5755

e-mail: GVKM07A-at-prodigy.com.






From: lmiller-at-ux1.cso.uiuc.edu
Date: Sat, 15 Apr 1995 11:56:28 -0700
Subject: Virus particle counting

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Message-Id: {199504151655.AA23238-at-ux1.cso.uiuc.edu}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Hi barbara,
I tried to post you personally, the mail bounced back. Probable reason for
some lack of response!!

We mixed a known concentration of latex spheres with a known volume of
virus culture. The sample was ultracentrifuged and resusupended with a
known volume of water. Then we mixed well and negative stained the samples.
Determine the counts using a ratio of # latex particles to known
concentration and using this ratio with the particle count.

Understanding that this is going on the assumption that latex spheres and
virus particles will have the same affinity for the formvar grid. (Some
problems will occur if the latex agglutinates.)

In this method, both a general ideal of concentration is reached, and the
size can be determined by comparison with the latex particle. A back up to
measuring the particle on a negative.

I've done this about 2X for researchers.

Good luck,
Lou Ann

***************************
Lou Ann Miller
Microscopic Imaging Lab
College of Vet. Medicine
University of Illinois
2001 S Lincoln Ave
Urbana,Illinois 61801
217-244-1566
lmiller-at-ux1.cso.uiuc.edu






From: Bilge Hakan Sen :      SEN-at-dishekimligi.ege.edu.tr
Date: Mon, 17 Apr 1995 12:56:01 TUR+2
Subject: ' Seeking Postdoctoral Position '

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To whom it may concern,
I am a research assistant professor in Department of Restorative
Dentistry,School of Dentistry,Ege University in Izmir,Turkiye since
1987. I have received my Ph.D. degree in endodontics in
February,1994.
I have been running a JEOL JSM-5200 scanning electron microscope in
our faculty for two years. I had also used a Philips SEM in
Amsterdam,The Netherlands for six months during my doctoral
studies.
I would like to continue my postdoctoral studies in biomedical
sciences by using SEM. Language will not
be a problem for me since I had graduated from Gar-Field High School
in Dale City,VA. Upon your request, I will be glad to send you my CV.
My researches by using SEM are:
- Anatomical approaches to dental pulp and periodontal ligament,
- Observation of microflora in dental root canals and dentinal
tubules,
- Observation of root surfaces in teeth with periapical resorption,
- Adaptation and penetration of root canal sealers into dentinal
tubules,
- Morphological changes in red blood cells of patients with diabetes
mellitus,
- Local effect of tetracycline on teeth with periodontitis,
- SEM investigations on natal and neonatal teeth,
- The effect of polishing on dental filling materials,
- Interactions of yeasts with dentin,
- The effect of titanium tetrafluoride on dental hard tissues,
- Durability of titanium tetrafluoride on enamel after in vivo
applications,
- Resorption patterns of decidous teeth.

Hope to collaborate with you.
Kind regards,
Dr. Bilge Hakan Sen







From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Mon, 17 Apr 1995 11:51:57 -0500 (CDT)
Subject: gluing sapphire

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From: AlliedHT-at-aol.com
Date: Sat, 15 Apr 1995 03:43:15 -0400
Subject: Re: gluing sapphire

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Try using LocTite 460 glue. It seems to be the highest quality super glue on
the market. You must buy it through a distributor. You can contact me for
that information.

Gary Liechty
310-635-2466




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Mon, 17 Apr 1995 11:53:19 -0500 (CDT)
Subject: Microtome for Bones

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From: jswafford-at-CCTR.UMKC.EDU
Date: Sun, 16 Apr 1995 14:25:41 CST
Subject: bone microtomes

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Does anyone know of the availability of a used microtome capable of cutting
undecalcified bone- something similar to the Reichert Autocut, Polycut etc?
Also, is there a server on the network for histochemical people?
Jim Swafford
School of Dentistry
University of Missouri
Kansas City, MO
muchas gracias and many thanks!




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Mon, 17 Apr 1995 11:53:43 -0500 (CDT)
Subject: gluing sapphire

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From: Brian G. Demczyk :      demczyk-at-ERXINDY.rl.plh.af.mil
Date: Mon, 17 Apr 1995 08:50:26 +0059 (EDT)
Subject: Re: Gluing Sapphire

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What's wrong with M-Bond 610. I've used it for years in preparing
cross sections, including sapphire.X






From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Mon, 17 Apr 1995 12:01:19 -0500 (CDT)
Subject: Please use the Stnd Address

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G'day Subscribers....


You may notice that Microscopy Email appears
to be coming from a new address called

MicroscopyListZaluzec-at-aaem.amc.anl.gov...


PLEASE PLEASE, DO NOT use this address when
posting to the list. Continue to use

Microscopy-at-AAEM.AMC.ANL.GOV

It is a temporary forwarding address that I
am using to help clear up the looping that
happened last week. Over the weekend one
person tried to post to that address and
started another loop. I caught it, but
it still took me the morning to clear up
things. Just post as you always did in the
past.

Thanks.... Nestor





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Mon, 17 Apr 1995 11:54:01 -0500 (CDT)
Subject: looking for an old book

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From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Mon, 17 Apr 1995 11:52:23 -0500 (CDT)
Subject: Virus particle counting

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From: lmiller-at-ux1.cso.uiuc.edu
Date: Sat, 15 Apr 1995 11:56:28 -0700
Subject: Virus particle counting

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Hi barbara,
I tried to post you personally, the mail bounced back. Probable reason for
some lack of response!!

We mixed a known concentration of latex spheres with a known volume of
virus culture. The sample was ultracentrifuged and resusupended with a
known volume of water. Then we mixed well and negative stained the samples.
Determine the counts using a ratio of # latex particles to known
concentration and using this ratio with the particle count.

Understanding that this is going on the assumption that latex spheres and
virus particles will have the same affinity for the formvar grid. (Some
problems will occur if the latex agglutinates.)

In this method, both a general ideal of concentration is reached, and the
size can be determined by comparison with the latex particle. A back up to
measuring the particle on a negative.

I've done this about 2X for researchers.

Good luck,
Lou Ann

***************************
Lou Ann Miller
Microscopic Imaging Lab
College of Vet. Medicine
University of Illinois
2001 S Lincoln Ave
Urbana,Illinois 61801
217-244-1566
lmiller-at-ux1.cso.uiuc.edu






From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Mon, 17 Apr 1995 11:54:18 -0500 (CDT)
Subject: looking for an old book

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From: Arthur_Strange-at-mail.magic.ca
Date: Sun, 16 Apr 1995 10:41:24 EST
Subject: Re: Looking for a old book

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Hi Debbie; Re: "MICROCOSMOS" BY Jeremy Burgess , Michael Marten and
Rosemary Taylor.
This was First published in 1987 by Cambridge University Press and in 1990
republished under the tile of "UNDER THE MICROSCOPE'.

I see that you live in Peterborough you should be able to find it at The
Worlds Biggest Bookstore in Toronto, I saw it there some time last year.

Another source for the book where they have copies of both editions listed
is:

SAVONA BOOKS
9 Wilton Road,
Hornsea,
North Humberside,
HU18 - 1QU
England UK

The current catalogue lists the 1987 edition at 27 pounds sterling, and the
1990 edition at 15 pounds sterling.

For general interest to all on the list, SAVONA BOOKS has an extensive
catalogue, some fifty pages long with as many topic areas of rare, old,
and new books on microscopy and related subjects. I have had a number of
books from them and they all arrive safely on this side of the pond.

Happy reading,

Arthur





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Mon, 17 Apr 1995 11:55:10 -0500 (CDT)
Subject: Viral Particle Counting

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From: Joseph P. Neilly 708-938-5024 :      NEILLY.JOSEPH-at-igate.abbott.com
Date: Mon, 17 Apr 1995 08:50:00 -0600 (CST)
Subject: Re:Viral Particle Counting

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Mr-Received: by mta RANDB; Relayed; Mon, 17 Apr 1995 09:16:22 -0600
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Content-Return: prohibited

Barbara Hartman asked for a technique for viral particle counting in the
TEM. We routinely use a direct sedimentation/negative staining method
for doing particle counts (see Miller M.F. and Rdzoc E.J., Proc. 39th
Ann. Mtg. EMSA, p. 404 (1981) and Miller M.F., Proc. 37th Ann. Mtg.
EMSA, p. 404 (1979)). This technique uses a Beckman Airfuge and a
specially designed rotor (Model EM90) which produces uniform
sedimentation across the grid, a potential source of error with some
rotor designs. It is relatively easy to do if you have an airfuge and
rotor. Hope this helps.

Joe Neilly
Abbott Laboratories
North Chicago, IL 60064
709-938-5024
E-mail: Joseph.neilly-at-abbott.com






From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Mon, 17 Apr 1995 15:47:25 -0500 (CDT)
Subject: RE-TechBooks Addr

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From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 17 Apr 1995 14:31:17 -0400
Subject: RE-TechBooks Addr

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Message-ID: {n1413993084.2966-at-mse.engin.umich.edu}

Subject: Time: 2:27 PM
OFFICE MEMO RE:TechBooks Addr Date: 4/17/95

On two occasions TechBooks have produced copies of out-of-print books for me,
and have done a truly high quality job of it. The phone number I have for
them is 800-767-1518.





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Mon, 17 Apr 1995 15:48:30 -0500 (CDT)
Subject: TEM Disc Grinder

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From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Mon, 17 Apr 1995 15:54:11 -0500 (CDT)
Subject: Another old TEM available

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From: Doug Davis :      doug_davis-at-maillink.berkeley.edu
Date: 17 Apr 1995 08:59:28 -0700
Subject: Another old TEM available

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Message-ID: {n1414013206.5297-at-maillink.berkeley.edu}

Subject: Time: 7:57 AM
OFFICE MEMO Another old TEM available Date: 4/17/95

Philips 200 at UC Berkeley, has not run in two years.

Contact: Prof. Ed Sylvester at (510) 642-7353 or Ethel Nakamura in Wellman
Hall at (510) 642-1603 for details.
Some funds available for moving, Ask Ed.





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Mon, 17 Apr 1995 15:46:53 -0500 (CDT)
Subject: Re: Residual Gas Analyzer

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From: minter-at-Kodak.COM (John Minter)
Date: Mon, 17 Apr 1995 14:08:06 -0500
Subject: Re: Residual Gas Analyzer

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John Fournelle wrote:

} I would be interested in hearing experiences/suggestions folks have
} with Residual Gas Analyzers on their electron microscopes (specifics
} please). (I have a Cameca SX50/51 electron microprobe that I am thinking
} about installing one on.)

We have a Spectra VacScan Model 100 RGA permanently attached to
our Philips CM-20 that is used primarily for cryoTEM. The ability
to measure the partial pressure of contaminants (especially water
vapor) makes it easy to track down problems. We bought this through
Philips when we bought the microscope. We chose this one because it
is the model they send to their service engineers when they need one.
Our service engineer really like having this as a diagnostic tool.
Given the high price most of us pay for our microscopes, I can't understand
why more labs don't have this accessory that costs less than $10k.

Best Regards,
John

John R. Minter, Ph. D. Phone: (716) 722-3407
Eastman Kodak Company FAX: (716) 477-3029
Analytical Technology Division email: minter-at- kodak.com
Rochester, NY 14562-3712






From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Mon, 17 Apr 1995 11:56:23 -0500 (CDT)
Subject: viral particle counting

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From: EMLAB-at-opus.mco.edu
Date: Mon, 17 Apr 1995 11:35:53 -0500 (EST)
Subject: Re: VIRAL PARTICLE COUNTING

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Dear Barbara,

I have preformed many many viral particle counts, mainly with enteric virus.
The few references I have concerning particle counting are as follows:


Kinetic Attachment Method:

1) Sharp, D.G., 1974 Procedings of 32nd Annual Meeting of EMSA. Clayton's
Publishing Division, p. 264-265

This methods describes using Al coated grids to allow virus to settle
out onto the grids.


Pseudoreplication Method:

2) Smith,K.O. and Benyesh-Melnick,M., 1961 Proc. Soc. Exptl. Biol. 107:409-413.

3) Smith,K.O. and Melnick,J.L., 1962 J. Immunol. 89:279-284.

4) Smith,K.O. and Melnick,J.L., 1962 Virology 17:480-490

5) McCombs,R.M., Benyesh-Melnick,M. and Brunschwig,J.P., 1966 J. Bacteriol
91:803-812.

6) Benyesh-Melnick,M., et al 1966 J. Bacteriol. 92:1555-1561

The first method works vewry well with highly purified preps while the second
method is great for cell culture supernatants, lysates or stool specimens.
The first method is much more accurate and precise while the second is for
good ball park estimations.

The one comment that I saw on the microscopy listserver about adding latex
spheres to the viral prep and counting virus/spheres in my opinion is not a
good suggestion only because of the different diffusion/settling constants
between virus and spheres.

One comment on the counts obtained. You will probably be counting all viral
particles and not just infectious ones, sokeep this in mind.

Good Luck,
Ed Calomeni
Medical College of Ohio
Toledo, OH
emlab-at-opus.mco.edu





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Mon, 17 Apr 1995 15:53:36 -0500 (CDT)
Subject: Re: gluing sapphire

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From: modum-at-Gatan.com (Michael Odum)
Date: Mon, 17 Apr 1995 10:09:12 -0700
Subject: Re: gluing sapphire

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Message-Id: {199504171607.JAA06923-at-core.gatan.com}

Hello,

I think that you are having trouble with the G1 epoxy for one or two
reasons. First the epoxy is not bonding because the sapphire is not clean.
When I make cross-sections I always use a cotton swab and a little acetone
to clean my wafers right before putting them into a stack. Even if I have
cleaned them previously.

The second reason may be that the G1 is being mixed wrong. It needs to
be measured by weight in a 10:1, epoxy-to-hardener ratio. The 10 drops/1
drop instructions on the lid of the cross-sectioning kit have misled a lot
of people. It is something that should have been fixed in the packaging
long ago, and I am sorry if it has confused you.

If you are still having problems after this I would suggest getting a
new batch of epoxy. It is possible that the epoxy is too old, or has been
stored in an enviroment not suitable for long-term sitting.

Please feel free to contact me or, Dr. Alani, if you have an other questions.
Thanx.

Michael W. Odum
Gatan, Inc.
6678 Owens Dr.
Pleasanton, CA 94588
Tel: 510-463-0200
Fax: 510-463-0204
E-Mail: modum-at-gatan.com

Reza Alani
E-Mail: ralani-at-gatan.com



} Does anybody know a method of mounting sapphire to make cross-
} sections for TEM ?
}
} We have used the GATAN G1 EPOXY glue whitout success.
}
} We know a lot of methods to prepare cross-section specimens. We are
} able to prepare samples in plane without difficulty and cross-sections with
} different materials but sapphire.
}
} It seems it would be the adhesion of the glue on sapphire which is the
} problem with our cross-section samples. Any idea on the type of glue that
} should be used.
}
} Thank you.
} Georges Veilleux
} Researcher
} INRS-Energie et materiaux
} C.P. 1020
} Varennes, Qc
} Canada
} J3X 1S2
} Tel.: (514) 929-8110
} Fax: (514) 929-8102





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Mon, 17 Apr 1995 11:55:57 -0500 (CDT)
Subject: OsO4

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From: SUSAN R. SESACK :      SESACK-at-brain.bns.pitt.edu
Date: Sun, 16 Apr 1995 12:17:10 EDT
Subject: Re: OsO4 Oxidation

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} Date: Fri, 14 Apr 1995 20:51:14 -0500 (CDT)
} From: "Nestor J. Zaluzec-Argonne Nat. Lab." {ZALUZEC-at-AAEM.AMC.ANL.GOV}
} To: MICROSCOPYLISTZALUZEC-at-AAEM.AMC.ANL.GOV
} Cc: ZALUZEC-at-AAEM.AMC.ANL.GOV
} Subject: OsO4 Oxidation

} From: SMTP%"GVKM07A-at-mail.prodigy.com" 14-APR-1995 03:25:23.55
} To: MICROSCOPY
} CC:
} Subj: Fwd: OsO4 'oxidation'
}
} I would like to respond as follows:
}
} The valence state of Os in the tetroxide is presumed to be +8.
}
} As the valence state is reduced to lower valence states there is a
} whole series of color changes, and by the time it gets to +2, the color
} is black.
}
} When one has a vial that has started to turn brown, more likely than
} not, it is not a unique valence state but a mixture of valence states,
} or some purists might say a distribution of valence states and
} continues to become more and more black in color as the extent of
} reduction proceeds further.
}
} At that point, in the ampoule, the change is irreversible and there is
} nothing anyone can do to reverse that change (without breaking open the
} ampoule) and doing actual chemistry to reverse the reactions..
}
} Here are some options to discarding the spoiled (for some applications)
} ampoules::
}
} 1] Some people could still make use of the aqueous osmium , for
} example, those doing vapor phase staining of rubber modified polymers.
} For such work, it does not really matter that much whether the solution
} is "100% active" or not. It is the vapors that count. This might not
} be true for everyone but at least it is one reason why you need not
} automatically think of sending it off to some kind of hazardous waste
} container. I would imagine there would be student uses for the "brown"
} osmium product as well.
}
} 2] If you absolutely have no use for it and if you have some quantity
} of ampoules, the osmium can be recycled. Or even if you don't have a
} whole lot, we can still combine it with other ampoules and recycle the
} reduced osmium. Contact me by e-mail if you wanted to explore that
} option. Osmium is a non-renewable resource and we should try to
} preserve it. The osmium you would send back might not have any real
} economic value in the sense that you would get anything back for it,
} but it is usually a lower cost alternative relative to the cost of
} disposing of it as a hazardous waste.
}
} Charles A. Garber
} SPI Supplies
} PO Box 656
} West Chester, PA 19380
}
} Ph: 1-(610) 436-5400 FAX:1-(610) 436-5755
}
} e-mail: GVKM07A-at-prodigy.com.

Dr. Garber,

I had no idea that you could recycle osmium or that is was a
non-renewable resource. I suppose I could have figured this out but
never thought about it. We collect the osmium that we use to fix
tissue sections in waste containers and have them picked up when
they're full. We also occasionally discard osmium that we've diluted
with phosphate buffer once it gets beyond a certain age. Are you
only interested in recycling un-opened ampoules, or are these other
forms also of value? The jug of waste osmium eventuall turns
completely to a black solid in a clear liquid. I don't care about
getting any money back. My only monitary concern is the cost of
hazardous chemical shipment. Please let me know whether it would be
worth my initiating a recycling policy in my lab. Thanks.

S.
sesack-at-bns.pitt.edu
Susan R. Sesack
Dept. Neuroscience
University of Pittsburgh
Pittsburgh, PA 15260





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Tue, 18 Apr 1995 9:07:56 -0500 (CDT)
Subject: Residual Gas Analyser

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From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Tue, 18 Apr 1995 9:08:36 -0500 (CDT)
Subject: TEM: LocTite 460 super glue

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From: {ZALUZEC-at-AAEM.AMC.ANL.GOV}:ddn:wpafb
Date: 4-17-95 2:02pm
Subject: TEM: LocTite 460 super glue

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Message-Id: {9504171927.AA04601-at-riker.ml.wpafb.af.mil}
To: WALCKSD:ml:wpafb, SCHELTFJ:ml:wpafb
Subj: gluing sapphire
Orig-Author: {"Nestor J. Zaluzec-Argonne Nat. Lab."
{ZALUZEC-at-AAEM.AMC.ANL.GOV} }:ddn:wpafb
-----------------------------------------------------------



From: AlliedHT-at-aol.com
Date: Sat, 15 Apr 1995 03:43:15 -0400
Subject: Re: gluing sapphire

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Try using LocTite 460 glue. It seems to be the highest quality super glue on
the market. You must buy it through a distributor. You can contact me for
that information.

Gary Liechty
310-635-2466






From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Tue, 18 Apr 1995 9:16:03 -0500 (CDT)
Subject: Univ of Nebrasaka EM Lab

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From: tvoiles-at-unlinfo.unl.edu (Todd Voiles)
Date: Tue, 18 Apr 1995 10:00:04 -0400
Subject: Univ of Nebrasaka EM Lab

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Message-Id: {9504181500.AA02970-at-unlinfo.unl.edu}
X-Sender: tvoiles-at-129.93.1.11
X-Mailer: Windows Eudora Version 1.4.3
Mime-Version: 1.0
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Hi,


I'd just like to inform all those persons from the U of Nebraska that
happen to be on this list that the EM Lab is starting a "newsletter" of
sorts and an electronic scheduling system. Please send me a
note - tvoiles-at-unlinfo.unl.edu- if you wish further information.

Center for Materials Research and Analysis
Central Facility for Electron Microscopy
University of Nebraska at Lincoln

Todd Voiles
tvoiles-at-unlinfo.unl.edu





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Tue, 18 Apr 1995 10:26:51 -0500 (CDT)
Subject: Sources for RGA's

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From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 18 Apr 1995 12:14:02 -0400
Subject: RE-Sources for RGAs

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Message-ID: {n1413915191.89693-at-mse.engin.umich.edu}

Subject: Time: 12:08 PM
OFFICE MEMO RE:Sources for RGAs Date: 4/18/95

When I was finishing up my book on Vacuum Methods I made a survey of the
sources of RGAs and found more than a dozen companies sell them (probably not
all are manufacturers, however). These are listed in Appendix 2, p. 468, and
their addresses are given in Appendix 3. There is also a discussion of the
functioning of RGAs and some interesting examples of their application to
problems in EM in Section 3.4.1, p. 114.





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Tue, 18 Apr 1995 9:14:22 -0500 (CDT)
Subject: Residual Gas Analysis

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From: Keith Ryan :      KPR-at-wpo.nerc.ac.uk
Date: Tue, 18 Apr 1995 10:11:00 +0000
Subject: Residual Gas Analysis

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Message-Id: {sf938fea.041-at-wpo.nerc.ac.uk}
X-Mailer: Novell GroupWise 4.1

Residual Gas Analysis - I missed the original John Fournelle message but
caught the John Minter reply.

I spent some time in the mid-80's using an Anavac-2 mass spectrometer
from VG Gas Analysis, Cheshire, UK. This was mounted on both a JEOL
35C SEM and a JEOL 200CX STEM (obviously not simultaneously!).

The system was amazingly sensitive and showed rise and fall of
hydrocarbons as the pumping system liquid nitrogen traps were filled and
later emptied, or when specimen-area anti-contaminators were cooled or
warmed. We actually wanted to monitor water vapour in cryo-setups, and
in this regard it was again very useful.

A small paper was published in the now defunct Austrian joournal
Mikroskopie (Wien) 42: 196-205 by KP Ryan, DH Purse & JW Wood titled:
Cryo-stage performance and observation of freeze-drying in a scanning
electron microscope. It shows a few graphs of water vaour partial pressure
in various ways. Those were the days!

The instrument was very useful also for tracing vacuum leaks, tune it for
helium (by letting some in via the airlock) and then probe around the
column with a fine jet from a hypodermic needle or something similar. Mail
again if I can be of further help - although it is now receding in the past!
Good luck. Keith Ryan





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Tue, 18 Apr 1995 12:08:34 -0500 (CDT)
Subject: Microtome for Bone

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From: South Bay Technology :      73531.1344-at-compuserve.com
Date: 18 Apr 95 13:46:43 EDT
Subject: Microtome for Bones

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While I do not know of any microtomes such as what you mentioned, we do produce
a line of cutting and polishing equipment that is ideally suited for
undecalcified bone. We produce diamond wheel saws and diamond band saws that
are used extensively for this purpose.

If you would like more detailed information, please contact me either by e-mail
or at:

South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673 USA

TEL: 800-728-2233 (toll free in USA)
714-492-2600
FAX: 714-492-1499





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Tue, 18 Apr 1995 12:09:17 -0500 (CDT)
Subject: EM Technician Position

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From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Tue, 18 Apr 1995 12:35:14 -0500 (CDT)
Subject: Disk Grinder

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From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Tue, 18 Apr 1995 9:12:32 -0500 (CDT)
Subject: Looking for old books

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From: morten.laane-at-bio.uio.no (Morten M. Laane)
Date: Tue, 18 Apr 1995 08:59:32 +0100
Subject: Re: Looking for a old book

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Message-Id: {199504180745.JAA19168-at-darwin.uio.no}
Mime-Version: 1.0
Content-Type: text/plain; charset="NS_4551-1"
Content-Transfer-Encoding: quoted-printable

} Date: Thu, 13 Apr 1995 15:06:11 -0400 (EDT)
} From: DLIETZ-at-TrentU.ca
} Subject: books for sale
} To: microscopy-at-aaem.amc.anl.gov
} Message-id: {01HPALSGL9E400DE1O-at-TRENTU.CA}
} X-VMS-To: IN%"microscopy-at-aaem.amc.anl.gov"
} MIME-version: 1.0
} Content-type: TEXT/PLAIN; CHARSET=3DUS-ASCII
} Content-transfer-encoding: 7BIT
}
} Recently, I had seen a book entitled "MICROCOSMOS" BY Jeremy Burgess ,
} Michael Marten and Rosemary Taylor . The book is now out of print and I'm
} interested in purchasing a second hand book. I work at Trent University
} and often do high school tours for the area. I thought this type of book
} would peek the interests of those students as well as lower year
} university students into the field of microscopy. If anyone would like to
} part with their copy or know where I can purchase a copy please reply. Or
} if there is a title of a more recent edition to the book recently in print
} please forward .
} THANKS DEBBIE

Sir! I have this book in my library. My specimen was bought at the
university book store here called "Akademica" , fax no. 22 85 30 53 , p.o.
Box 84- 0314 Oslo,Norway. As they keep lots of books, there still may be a
copy left. The book is ISBN 0-521-30433-4. and was published in 1987.
That s not so old. We have both the original Hooke s "Micrographia" and
Leeuvwenhookes "Achana Naturae" in two volumes here (1695) and most of what
have been published ever. Sincerely yours Morten M.Laane , Professor of
Biology, University of Oslo.






From: Ian Parkinson :      IPARKINS-at-immuno.imvs.sa.gov.au
Date: Wed, 19 Apr 1995 13:37:22 GMT+1030
Subject:

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Message-Id: {9504190406.AA25707-at-huon.itd.adelaide.edu.au}

UNSUBSCRIBE


Ian Parkinson
Division of Tissue Pathology
Institute of Medical and Veterinary Science
Frome Road
Adelaide
South Australia 5000
Australia.




From: CAROL ANNE COOKE :      cooke-at-welchlink.welch.jhu.edu
Date: Wed, 19 Apr 1995 10:52:03 -0400 (EDT)
Subject: subscription

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Please subscribe.





From: CAROL ANNE COOKE :      cooke-at-welchlink.welch.jhu.edu
Date: Wed, 19 Apr 1995 13:12:11 -0400 (EDT)
Subject: Another test

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Hi,
Would someone please send me a quick message, I'm
trying to test this new subscription. Is there a special
procedure for Macintosh cpu's through welchlink?

Thanks
C. Cooke





From: Peru Laurence :      perul-at-ERE.UMontreal.CA
Date: Wed, 19 Apr 1995 13:18:20 -0400 (EDT)
Subject: asking for informations

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Hello

I heard about your news group from a friend.
I am working with TEM, SEM and microanalysis on biological materials. I
think I should have interesting informations with your list.
How can I subscribe ?

Sincerely

Laurence Peru - Perul-at-ERE.UMontreal.Ca

Laboratory for Electron Microscopy
Faculty of Dental Medicine
Universite de Montreal
2900 Edouard Montpetit
Montreal, QC, Canada - H3C 3J7






From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Thu, 20 Apr 1995 9:56:10 -0500 (CDT)
Subject: Lets Try a Restart Again!

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G'day Colleagues

Yes I have seen all your questions as to the
fact your not receiving mail. I once again shut
things down. This time it was due to a major change
in the Email router here at ANL. The new configuration
completely swamped my host here, because, unlike the
old router, when a message was delayed for more than
4 hours the router started sending messages back to
the originator.

I started getting ~ 100 warning messages each day
saying that the mail message to XXXX.YYYY.ZZZZ
was not sent for 4 hours and a new attempt will
be made in another 4 hours..

I'm going to try to restart today and see if the
message problem is cured.

Keep posting your messages to Microscopy-at-aaem.amc.anl.gov
I will intercept each and repost it after a short delay.

Sigh... Nestor






From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Thu, 20 Apr 1995 9:58:04 -0500 (CDT)
Subject: Ruthenium Red in freeze-sub

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From: SGKCCK-at-aol.com
Date: Tue, 18 Apr 1995 18:28:21 -0400
Subject: Ruthenium Red in freeze-sub

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Doug,
Ok got your message and I see the problem with DMSO although it would have
been nicer to work with. Please let me know if lunch helped generate any
other ideas. Have you tried the THF yet? Please let me know. I am still
searching for other alternatives and will let you know if something comes up.
Stacie




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Thu, 20 Apr 1995 9:59:53 -0500 (CDT)
Subject: Re: TEM: LocTite 460 super glue

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From: Brian G. Demczyk :      demczyk-at-ERXINDY.rl.plh.af.mil
Date: Wed, 19 Apr 1995 09:55:38 +0059 (EDT)
Subject: Re: TEM: LocTite 460 super glue

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Superglue is unstable under the electron beam!!!






From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Thu, 20 Apr 1995 10:00:35 -0500 (CDT)
Subject: Unicryl

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From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Thu, 20 Apr 1995 9:56:52 -0500 (CDT)
Subject: JEOL Scope For Sale

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From: Larry Hawkey :      hawkey-at-neuro.duke.edu
Date: Tue, 18 Apr 1995 15:11:29 -0400
Subject: FOR SALE JEOL 1200 exII TEM

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JEOL 1200exII Conventional TEM (straight TEM with no add-ons). This
instrument is less than five years old, has been under service contract the
entire time, and is in mint condition. It has had limited users and all were
closely supervised. As a result, this machine has had no down time.
For more information, call Larry Hawkey (919-6841-6425) or E-Mail
(hawkey-at-neuro.duke.edu)

A real jem of a JEOL.




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Thu, 20 Apr 1995 9:57:07 -0500 (CDT)
Subject: Microtome for Bones

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From: South Bay Technology, 73531,1344
Date: 18 Apr 95 14:05:15 EDT
Subject: Microtome for Bones

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---------- Forwarded Message ----------


RE: Copy of: Microtome for Bones

While I do not know of any microtomes such as what you mentioned, we do produce
a line of cutting and polishing equipment that is ideally suited for
undecalcified bone. We produce diamond wheel saws and diamond band saws that
are used extensively for this purpose.

If you would like more detailed information, please contact me either by e-mail
or at:

South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673 USA

TEL: 800-728-2233 (toll free in USA)
714-492-2600
FAX: 714-492-1499





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Thu, 20 Apr 1995 9:57:48 -0500 (CDT)
Subject: EM Salaries

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From: delannoy-at-welchlink.welch.jhu.edu (Michael Delannoy)
Date: Tue, 18 Apr 1995 14:28:44 +0500
Subject: EM Salaries

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} To: Microscopy
} From: delannoy-at-welchlink.welch.jhu.edu (Michael Delannoy)
} Subject: EM Salaries
}
} Hello,
} I'm trying to get some information on salary ranges for
} electron microscopists and EM core facility managers. Is there a
} source I can check? Thank you.
}
} M. Delannoy
}





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Thu, 20 Apr 1995 9:58:29 -0500 (CDT)
Subject: osmium tetroxide oxidation

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From: SGKCCK-at-aol.com
Date: Tue, 18 Apr 1995 18:43:03 -0400
Subject: osmium tetroxide oxidation

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As we discussed today, there is a pretty interesting paper which is out that
deals with your recent correspondance on the above subject. I have sent you a
reprint of the article entitled "Pro's for a multiple/repeated use and
disposal with recovery of osmium tet. solutions in routine e.m.. This paper
was presented at the 1992 Boston EMSA meeting. It makes for very interesting
reading and gives you a whole lot of alternatives of what to do with the
Osmium. If you have any questions just let me know for I am in contact
constantly with the gentlemen that did the work.
If anyone else is interested in a copy of this protocol please contact me and
I will be more then happy to send you one.
Stacie Kirsch
Electron Microscopy Sciences
215-646-1566




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Thu, 20 Apr 1995 9:59:35 -0500 (CDT)
Subject: CPD of millipore filters

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From: t.bostrom-at-qut.edu.au (Thor Bostrom)
Date: Wed, 19 Apr 1995 18:54:45 +1000
Subject: CPD of millipore filters

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A staff member here would like to critical point dry millipore filters
containing bacteria and zooplankton for SEM. She is concerned about
losing the fine material, and was wondering if the filters could be
mounted in some special holder in the CPD. Has anyone tried doing this,
and if so, what sort of setup would you need to hold the filters?

Thanks for any suggestions,
Thor

Dr Thor Bostrom
Analytical EM Facility
Queensland University of Technology (QUT)
GPO Box 2434, Brisbane, QLD 4001, Australia
Ph: 61-7-8642351 FAX: 61-7-8645100





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Thu, 20 Apr 1995 10:00:47 -0500 (CDT)
Subject: BDMA questions

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From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Thu, 20 Apr 1995 10:01:21 -0500 (CDT)
Subject: Tripod Polishing Sapphire for TEM

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From: South Bay Technology :      73531.1344-at-compuserve.com
Date: 19 Apr 95 15:21:31 EDT
Subject: Tripod Polishing Sapphire for TEM

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Dear Fellow Tripodders-

I am looking for any information I can find concerning Tripod Polishing of
Sapphire. I have spoken to a few people who have had some success, but even
they would like to hear other suggestions. If anyone out there has any
information, please respond to me directly. I'll summarize the responses for
the listserver.

Thank you!

David Henriks
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673 USA

TEL: 800-728-2233 (toll free in USA)
714-492-2600
FAX: 714-492-1499





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Thu, 20 Apr 1995 10:01:41 -0500 (CDT)
Subject: cryo-ultramicrotomy of macrophages

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From: Jaime Dant :      jaime-at-borcim.wustl.edu
Date: Wed, 19 Apr 1995 16:01:09 -0500
Subject: cryo-ultramicrotomy of macrophages

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Hello. I am currently having difficulty getting cryo sections of mouse bone
marrow macrophages to stick on grids.

I am using conventional techniques with a dry diamond knife, picking the sections up with sucrose and floating the grids on buffer before further prossing
with immunogold. I have been using this method for some time with different
types of cells and intracellular parisites with no difficulty. My problem
seems to be with just this one cell type...

Any advise from someone in the know?

Thank you.

Jaime A. Dant
Washington University School of Medicine
St. Louis
jaime-at-borcim.wustl.edu




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Thu, 20 Apr 1995 10:02:39 -0500 (CDT)
Subject: microwaving coplin jars

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From: russb-at-xactdata.com (Russell Berkheimer)
Date: Thu, 20 Apr 1995 15:38:25 -0700 (PDT)
Subject: wayward email

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Hi -

My machine serves as an internet Gateway for 'space.com'.
My machine has been receiving email with an incorrectly
specified Fully Qualified Domain Name (FQDN).

email was addressed to:

jegiles-at-honeywell.space.com

other possible addresses:

...-at-space.honeywell.com {== probable address
...-at-space.lockheed.com

Thanks -



Russ Berkheimer, Chief Engineer | email: russb-at-xactdata.com
XactData Services, Inc. | voice: (206) 382-6618
701 Fifth Avenue, Suite 4850 | fax: (206) 382-6615
Seattle, WA 98104




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Thu, 20 Apr 1995 9:57:24 -0500 (CDT)
Subject: TEM Disc Grinder

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From: South Bay Technology :      73531.1344-at-compuserve.com
Date: 18 Apr 95 14:01:33 EDT
Subject: TEM Disc Grinder

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Patrick-

We produce a wide range of "disc grinders" for TEM and other applications. Our
lapping and polishing fixtures are ideally suited for TEM sample preparation.
In fact, we have a few polishing fixtures that will allow you to transfer the
sample mount from the polishing fixture, to any of our saws and also to our
dimpling instrument.

Our polishing fixtures which are designed specifically for TEM are:

Model 140 $ 785.00
Model 141 395.00

Either of these will allow you to transfer the sample holder as described above.
We also have larger fixtures for holding samples up to 2" in diameter. The
price range on these fixtures is from $345 - $995.

If you would like additional information, please contact me at:

South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673 USA

TEL: 800-728-2233 (toll free in USA)
714-492-2600
FAX: 714-492-1499

Best regards-

David Henriks





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Thu, 20 Apr 1995 9:59:04 -0500 (CDT)
Subject: NItrogen in EDS

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From: Keith R. Hallam :      k.r.hallam-at-bristol.ac.uk
Date: Wed, 19 Apr 1995 09:29:25 +0100 (BST)
Subject: Re: Nitrogen in EDS

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Message-Id: {9504190829.AA01870-at-zeus.bris.ac.uk}

}
} As a non specialist in EDS I have a question.
} Is it possible that our EDS detector (UTW from EDAX) attached to SEM does not
} "see" nitrogen peak at 0.91 keV (even in AlN and GaN it is hardly visible),
} though at the same time it detects easily very close C and O peaks?
} Leszek Kepinski
}
Leszek,

I don't know where I copied it from, but I wrote in my PhD thesis: "When
operating with the thin window in place, it is not possible to analyse for
nitrogen, as the carbon in the window heavily absorbs the nitrogen x-rays",
meaning the nitrogen x-rays are the right sort of energy to interact with
the carbon in your thin window, and only a few get through to be detected.
I haven't bothered figuring out more precisely what is happening.
Keith






From: ANLAEM::ZALUZEC Nestor J. Zaluzec-Argonne Nat. Lab. 19-APR-1995 10:20:56.45
Date: Thu, 20 Apr 1995 10:00:24 -0500 (CDT)
Subject: Re: TEM: LocTite 460 super glue

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From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Thu, 20 Apr 1995 10:02:19 -0500 (CDT)
Subject: Can Image calc. Shape Factor?

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From: Leo D Frawley 03 5667464 :      FRAWLEY-at-a1.resmel.bhp.com.au
Date: Thu, 20 Apr 1995 12:04:57 +1000
Subject: Can Image calc. Shape Factor?

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MR-Received: by mta VULCAN.MUAS; Relayed; Thu, 20 Apr 1995 12:04:57 +1000
MR-Received: by mta VULCAN; Relayed; Thu, 20 Apr 1995 12:05:10 +1000
Alternate-recipient: prohibited
Disclose-recipients: prohibited
Content-return: prohibited

Has anyone ever used NIH Image to calculate the shape factor of a sessile drop, and if so, how.
Calculation of the shape factor allows the surface tension of the droplet to be determined and requires the
accurate measurement of the radius of curvature on many points of the drop surface.

Thanks in advance.

Leo Frawley B.Sc
Electron Microscopist
BHP Research - Melbourne Labs
AARNet " frawley-at-a1.resmel.bhp.com.au "





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Thu, 20 Apr 1995 9:58:46 -0500 (CDT)
Subject: Residual Gas Analysis

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From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Tue, 18 Apr 1995 9:14:22 -0500 (CDT)
Subject: Residual Gas Analysis

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From: Keith Ryan :      KPR-at-wpo.nerc.ac.uk
Date: Tue, 18 Apr 1995 10:11:00 +0000
Subject: Residual Gas Analysis

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Message-Id: {sf938fea.041-at-wpo.nerc.ac.uk}
X-Mailer: Novell GroupWise 4.1

Residual Gas Analysis - I missed the original John Fournelle message but
caught the John Minter reply.

I spent some time in the mid-80's using an Anavac-2 mass spectrometer
from VG Gas Analysis, Cheshire, UK. This was mounted on both a JEOL
35C SEM and a JEOL 200CX STEM (obviously not simultaneously!).

The system was amazingly sensitive and showed rise and fall of
hydrocarbons as the pumping system liquid nitrogen traps were filled and
later emptied, or when specimen-area anti-contaminators were cooled or
warmed. We actually wanted to monitor water vapour in cryo-setups, and
in this regard it was again very useful.

A small paper was published in the now defunct Austrian joournal
Mikroskopie (Wien) 42: 196-205 by KP Ryan, DH Purse & JW Wood titled:
Cryo-stage performance and observation of freeze-drying in a scanning
electron microscope. It shows a few graphs of water vaour partial pressure
in various ways. Those were the days!

The instrument was very useful also for tracing vacuum leaks, tune it for
helium (by letting some in via the airlock) and then probe around the
column with a fine jet from a hypodermic needle or something similar. Mail
again if I can be of further help - although it is now receding in the past!
Good luck. Keith Ryan





From: Joyce Craig :      bafpjec-at-uxa.ecn.bgu.edu
Date: Fri, 21 Apr 1995 09:34:15 -0500 (CDT)
Subject: Re: EM Salaries

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IOn Thu, 20 Apr 1995, Nestor J. Zaluzec-Argonne Nat. Lab. wrote:

} From: SMTP%"delannoy-at-welchlink.welch.jhu.edu" 18-APR-1995 15:19:23.95
} To: MICROSCOPY
} CC:
} Subj: EM Salaries
}
} Date: Tue, 18 Apr 1995 14:28:44 +0500
} Message-Id: {9504181828.AA21435-at-welchlink.welch.jhu.edu}
} X-Sender: delannoy-at-welchlink.welch.jhu.edu
} Mime-Version: 1.0
} Content-Type: text/plain; charset="us-ascii"
} To: Microscopy-at-anlemc.msd.anl.gov
} From: delannoy-at-welchlink.welch.jhu.edu (Michael Delannoy)
} Subject: EM Salaries
} X-Mailer: {Windows Eudora Version 1.4.2b16}
} content-length: 291
}
} } To: Microscopy
} } From: delannoy-at-welchlink.welch.jhu.edu (Michael Delannoy)
} } Subject: EM Salaries
} }
} } Hello,
} } I'm trying to get some information on salary ranges for
} } electron microscopists and EM core facility managers. Is there a
} } source I can check? Thank you.
} }
} } M. Delannoy
} }
} Hi,
Statistics were taken by EMSA several years ago.
I can tell you that salaries are pathetic for techs, at least in my
experience. I just sent a man with 12 years of experience in histo and
EM pathology & research, a recent BS, and MSA Certification , to
Northwestern University for a position in downtown Chicago, and he was
offered $21,000 per year.





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Fri, 21 Apr 1995 10:19:19 -0500 (CDT)
Subject: Important Notice Read This!!!

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G'day Subscribers;

I've just finished reworking the mailing list.
I have now seperated out each group of users by
country code. This is the LAST message you
will receive using the original mailing list.
As soon as I'm sure that all copies of this message
have been sent out I will be disabling that old
list of subscribers and restarting a new list.

If by Monday AM you DO NOT receive another message
from me called TEST OF NEW LIST, then I have
accidently left your subscription off the reconfigured
list.

Please contact me at that time, at:

Zaluzec-at-aaem.amc.anl.gov

so that we can get you back on the system.


The mailing address of the listserver has not changed
it remains:

Microscopy-at-aaem.amc.anl.gov

You may continue to post all your messages to that
address. I will continue to intercept and redirect the
messages to the temporairy lists until I'm sure things
are back to some sense of normal (i.e. minimum errors) operation.

Cheers.. Nestor
Your Friendly Neighborhood SysOp.










From: John Warrack-1 :      John_Warrack-1%notes-at-sb.com
Date: 21 Apr 95 17:52:37 EDT
Subject: Re: CPD of millipore filters

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Message-Id: {9504211955.AA2171-at-pho018.sb.com}
To: MICROSCOPYLISTZALUZEC {MICROSCOPYLISTZALUZEC-at-AAEM.AMC.ANL.GOV}

Millipore filters can be successfully CPD'd in envelopes made from folded
filter paper, closed with a staple at the open edge. I use Whatman 541 4.25cm
papers routinely with 12mm filters. The sample code can be written on the paper
with pencil, and this mark can then be used to determine the "up" side of the
millipore filter - sometimes the sample is invisible to the eye.

John_Warrack-1%notes-at-sb.com

SmithKline Beecham UK






From: david.bright-at-NIST.GOV (David S. Bright)
Date: Fri, 21 Apr 1995 14:17:50 -0500 (EST)
Subject: WWW sites

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Anyone have good web sites for microscopy or image processing? The address
and a few words about it - topic, scope, usefulness - would be great.

Thanks
Dave

-------------------------------------------------------------
David S. Bright dbright-at-nist.gov
Microanalysis Research Group
Bldg. 222 (Chem.) A113
National Institute of Standards & Technology (NIST, formerly NBS)
Gaithersburg, MD 20899-0001 / USA
301-975-3911 (voice), 301-216-1134 (fax)
"You must have accurate and honest weights and measures, so that you may
live long in the land the Lord your God is giving you.", Deuteronomy 25:15






From: ANLAEM::ZALUZEC Nestor J. Zaluzec-Argonne Nat. Lab. 21-APR-1995 10:19:20.51
Date: Fri, 21 Apr 1995 13:00:11 -0500 (CDT)
Subject: Important#2 - from Nestor

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Hi Gang,

Just as I send out this posting the main DNS system
here at ANL crashed. About 1/2 of the sites did not
receive this message. So I apologize if you got
it twice, but for some of you (~ 800-1000) this will be your
first receipt.

Nestor



G'day Subscribers;

I've just finished reworking the mailing list.
I have now seperated out each group of users by
country code. This is the LAST message you
will receive using the original mailing list.
As soon as I'm sure that all copies of this message
have been sent out I will be disabling that old
list of subscribers and restarting a new list.

If by Monday AM you DO NOT receive another message
from me called TEST OF NEW LIST, then I have
accidently left your subscription off the reconfigured
list.

Please contact me at that time, at:

Zaluzec-at-aaem.amc.anl.gov

so that we can get you back on the system.


The mailing address of the listserver has not changed
it remains:

Microscopy-at-aaem.amc.anl.gov

You may continue to post all your messages to that
address. I will continue to intercept and redirect the
messages to the temporairy lists until I'm sure things
are back to some sense of normal (i.e. minimum errors) operation.

Cheers.. Nestor
Your Friendly Neighborhood SysOp.










From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sat, 22 Apr 1995 9:02:34 -0500 (CDT)
Subject: Test of New List

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Morning Microscopy Listserver Subscribers:

Here is the test of the new list.
Unless you receive another message from
me there is no need to reply to this message
just trash it.

Nestor





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sat, 22 Apr 1995 9:47:30 -0500 (CDT)
Subject: TEM: LocTite 460 super glue

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From: chandler-at-lamar.ColoState.EDU (John Chandler)
Date: Thu, 20 Apr 1995 11:39:53 -0700
Subject: Re: TEM: LocTite 460 super glue

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Message-Id: {v01510101abbc59d98c06-at-[129.82.126.28]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

}
} Superglue is unstable under the electron beam!!!

Would you explain a little bit what you mean by unstable? Do you mean
drifting specimen, or damage in the beam with release of sludge into the
column? I can learn to deal with some specimen drift for certain
specimens, but I don't want contamination.

Thanks for any input from anyone on the list.


John
chandler-at-lamar.ColoState.EDU
http://www.vetmed.colostate.edu/anatomy/faculty/chandler.html






From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sat, 22 Apr 1995 9:49:26 -0500 (CDT)
Subject: Microwaving Coplin Jars

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From: SGKCCK-at-aol.com
Date: Fri, 21 Apr 1995 07:00:46 -0400
Subject: Microwaving Coplin Jars

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Dear Dr. Robertson,
I saw your message on the e-mail regarding the above mentioned subject. From
what we understand you are currently using glass and yes I would agree that
over a short period of time they would begin to crack. With our microwave
and in conjunction with polyethylene coplin jars we have had much success.
The high density polyethylene does not crack nor rupture over many uses.
Our part # is 70319 if you have an interest.
If you have any further questions please do not hesitate to contact us.
Sincerely,
Stacie Kirsch
Electron Microscopy Sciences
P.O.Box 251
Fort Washington, Pa, 19034
Tel:215-646-1566
Fax:215-646-8931




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sat, 22 Apr 1995 9:49:48 -0500 (CDT)
Subject: Re: CPD of millipore filters

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From: Stephan Helfer :      S.Helfer-at-rbge.org.uk
Date: Fri, 21 Apr 1995 09:28:59 BST
Subject: Re: CPD of millipore filters

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I shouldn't think there are any problems. However, it might be
advisable to freeze dry part of the samples to compare the bacterial
/ plankton population. Filters can be cut up and prepared like thin
plant leaves, marking the top might be advisable [unless you want to
mount them vertically anyhow].
Yours sincerely


Dr Stephan Helfer, SSO
Mycologist

Royal Botanic Garden Edinburgh, Inverleith Row, EDINBURGH EH3 5LR,
Scotland UK

email STEPHAN-at-rbge.org.uk
phone: +44 (0)131 552 7171 ext 280
or +44 (0)131 459 0446-280 (direct digital VoiceMail line)
fax: +44 (0)131 552 0382




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sat, 22 Apr 1995 9:50:45 -0500 (CDT)
Subject: Re: osmium tetroxide oxidation

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From: EMLAB-at-opus.mco.edu
Date: Fri, 21 Apr 1995 08:51:03 -0500 (EST)
Subject: Re: osmium tetroxide oxidation

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Stacie,

How about posting the title, author(s) and journal of the reference you
mention in your posting of 2ndary use of OsO4.
Thanks,

Ed Calomeni
Medical College of Ohio
Toledo, OH
EMLAB-at-opus.mco.edu




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sat, 22 Apr 1995 9:51:08 -0500 (CDT)
Subject: Osmium Tetro. Protocol req.

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From: SGKCCK-at-aol.com
Date: Fri, 21 Apr 1995 06:22:51 -0400
Subject: Osmium Tetro. Protocol req.

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To all that has requested a copy of the protocol that I mentioned in my
E-Mail message regarding the recycling of osmium please note that I have
mailed to each of you a copy of the complete protocol.
If anyone has questions please do not hesitate to contact me.
Sincerely
Stacie Kirsch
Electron Microscopy Sciences
P.O.Box 251
Fort washington, Pa 19034
Tel: 215-646-1566
Fax:215-646-8931




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sat, 22 Apr 1995 9:51:23 -0500 (CDT)
Subject: EM Salary Surveys

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From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sat, 22 Apr 1995 9:51:54 -0500 (CDT)
Subject: microwaving coplin jars

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From: :      MELLIOTT-at-prl.pulmonary.ubc.ca
Date: Fri, 21 Apr 1995 06:53:48 +0800PST
Subject: microwaving coplin jars

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T. Robertson was asking about microwaving coplin jars. The answer is
not to use glass coplin jars as they do indeed tend to break. Use
plastic coplin jars available from a variety of scources. Any
reference I have seen says to use the plastic ones. We obtained ours
from either Fischer Scientific, or from Baxter-Canlab (now VWR-Canlab
) and have had no problems with them.

Yours,
Mark Elliott, PhD
UBC-Pulmonary Research Lab
Vancouver, Canada




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sat, 22 Apr 1995 9:48:31 -0500 (CDT)
Subject: grey levels - film vs digital

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From: tphillips-at-biosci.mbp.missouri.edu (Tom Phillips)
Date: Thu, 20 Apr 1995 12:12:08 -0500
Subject: grey levels - film vs digital

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Krivanek and Mooney (Ultramicroscopy 49:95-108, 1993) state "However,
whereas each pixel of the SSC (slow scan CCD) is capable of faithfully
capturing an intensity between 0 and 2(to the 14th power), each pixel of
film can capture only a few gray levels (roughly equivalent to the number
of silver grains that can fit into the area of one pixel without
overlapping)". Buonaquisti (Microscopy Today 95(1):12-13, 1995) states
film "is an anlog process and, as such, one can argue that sheet film has
an infinite gray scale resolution". Can anyone comment on this? I have
always thought more along Krivanek and Mooney's reasoning. Does anyone
know the number of grey levels film can capture? Thanks for any comments.


Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility
3 Tucker Hall
University of Missouri
Columbia, MO 65211
(314)-882-4712 (voice)
(314)-882-0123 (fax)






From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sat, 22 Apr 1995 9:49:05 -0500 (CDT)
Subject: CPD of Millipore membrane filters

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From: GVKM07A-at-prodigy.com (DR CHARLES A GARBER)
Date: Fri, 21 Apr 1995 02:35:23 EDT
Subject: CPD of Millipore membrane filters

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This is in response to posting from Dr. Thor Bostrom on April 20:

There is an easy solution:

We have been making for some years a product called "Microporous
Specimen Capsules". The original product had a pore size range of 120-
200 um, and is our part SPI #13215 and has overall dimensions of 10 mm
x 10 mm. However, you will have to cut out a small piece of the
membrane filter prior to insertion of the piece into the capsule.

We have a second product, exactly like the first, but with a pore size
of 78um which should be used if the features on the membrane at that
small.

We expect to have available yet a third product, just like the other
two, but with pore size on the order of 30 um.

Charles A. Garber
PRESIDENT
SPI SUPPLIES
PO BOX 656
WEST CHESTER, PA 19380

Ph: (610) 436-5400
(800) 2424-SPI

FAX:(610) 436-5755

e-mail: GVKM07A-at-prodigy.com





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sat, 22 Apr 1995 9:52:09 -0500 (CDT)
Subject: BDMA

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From: SGKCCK-at-aol.com
Date: Fri, 21 Apr 1995 06:34:54 -0400
Subject: BDMA

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Dear Karen,
I saw your message and I do have quite a bit of experience of uses with BDMA
and I have spoken for quite a few years with Audrey and alot of my customers
now do use BDMA in place of DMP-30 with great success. Please note the
following:
BDMA is less viscous than DMP-30,has a longer shelf life, and offers better
penetration of the tissue. In order to achieve the optimum results my
customers have all told me that when they use DMp the percentage is at 1.5-2%
final volume and with the BDMA it needs to be 2.5-3%. No greater than 3
however. One should warm the resin and the anhydride prior to mixing and as
well it would be beneficial to warm the stirring rod and the containers which
will be used for mixing.You should make up the lot fresh however many of my
customers has informed me that they have been storing the mixture at 4
degrees C in a bottle with a well fitting tight cap for several weeks and
have never experienced any difficulties and have achieved fine results. It
should be noted that if this is the avenue you will take prior to use the
mixture must be allowed to reach room temperature.
If you would like to delve deeper into this subject please just let me know.
I hope this gives you some info you were looking for.
Stacie Kirsch
Electron Microscopy Sciences
P.O.Box 251
Fort Washington, Pa. 19034
Tel:215-646-1566
Fax:215-646-8931




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sat, 22 Apr 1995 9:52:40 -0500 (CDT)
Subject: RE: microwaving coplin jars

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From: M.V. Parthasarathy :      mvp2-at-cornell.edu
Date: Fri, 21 Apr 1995 09:31:53 -0400 (EDT)
Subject: RE: microwaving coplin jars

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X-NUPop-Charset: English

In message Thu, 20 Apr 1995 10:02:39 -0500 (CDT),
"Nestor J. Zaluzec-Argonne Nat. Lab." {ZALUZEC-at-AAEM.AMC.ANL.GOV} writes:

} From: SMTP%"TROBERTS-at-eosin.path.uwa.edu.au" 19-APR-1995 22:49:39.09
} To: MICROSCOPY
} CC:
} Subj: microwaving coplin jars
}
***************

I don't know about coplin jars, but PARR INSTRUMENT COMPANY, 211 53rd St.,
Moline, IL 61265-1770 (Tel: 309 762-7716; Fax: 309-762-9453) sells microwave
acid digestion vessels that can be used for LM & EM mivrowave fixation. The
vessels are designed to provide complete containment of volatiles and stand
pressures up to 1200 psi and temperatures up to 250 C. Hope this helps.

*************************************************************************
M.V. Parthasarathy
Professor of Plant Biology & Director, EM Facility
Section of Plant Biology
228 Plant Science Building
Cornell University, Ithaca, NY 14850
Telephone: (607) 255-1734
Fax: (607) 255-5407
E-mail: mvp2-at-cornell.edu
***********************************************************************




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sat, 22 Apr 1995 9:50:26 -0500 (CDT)
Subject: EM Salaries

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From: Joyce Craig :      bafpjec-at-uxa.ecn.bgu.edu
Date: Fri, 21 Apr 1995 09:34:15 -0500 (CDT)
Subject: Re: EM Salaries

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IOn Thu, 20 Apr 1995, Nestor J. Zaluzec-Argonne Nat. Lab. wrote:

} From: SMTP%"delannoy-at-welchlink.welch.jhu.edu" 18-APR-1995 15:19:23.95
} To: MICROSCOPY
} CC:
} Subj: EM Salaries
}
} Date: Tue, 18 Apr 1995 14:28:44 +0500
} Message-Id: {9504181828.AA21435-at-welchlink.welch.jhu.edu}
} X-Sender: delannoy-at-welchlink.welch.jhu.edu
} Mime-Version: 1.0
} Content-Type: text/plain; charset="us-ascii"
} To: Microscopy-at-anlemc.msd.anl.gov
} From: delannoy-at-welchlink.welch.jhu.edu (Michael Delannoy)
} Subject: EM Salaries
} X-Mailer: {Windows Eudora Version 1.4.2b16}
} content-length: 291
}
} } To: Microscopy
} } From: delannoy-at-welchlink.welch.jhu.edu (Michael Delannoy)
} } Subject: EM Salaries
} }
} } Hello,
} } I'm trying to get some information on salary ranges for
} } electron microscopists and EM core facility managers. Is there a
} } source I can check? Thank you.
} }
} } M. Delannoy
} }
} Hi,
Statistics were taken by EMSA several years ago.
I can tell you that salaries are pathetic for techs, at least in my
experience. I just sent a man with 12 years of experience in histo and
EM pathology & research, a recent BS, and MSA Certification , to
Northwestern University for a position in downtown Chicago, and he was
offered $21,000 per year.





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sat, 22 Apr 1995 9:53:00 -0500 (CDT)
Subject: Re: Unicryl

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From: Luc Analbers :      L.J.S.Analbers-at-med.ruu.nl
Date: Fri, 21 Apr 1995 11:35:54 +0200
Subject: Re: Unicryl

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Hello Karen,

Always be carefull with using stuff like Unicryl or Lowicryl. If possible use
a fumehood. Otherwise (like we do) wear a full-face gas-mask with an apropriate
filter. Use the filter only once (every experiment).

I tried Unicryl, but after lots of disapointments we skipped to Lowicryl HM20.
(we used the plastic for immunno Electron Microscopy).
Do you get good results with Unicryl? If so, please let me know.

Bye.


Luc Analbers


***************************************************************************
* Luc Analbers * E-mail: Analbers-at-med.ruu.nl *
***************************************************************************
* Utrecht University * LLL *
* Medical Faculty * LLL *
* Dept. Medical Physiology & * LLL A *
* Sportsmedicine * LLL AA AA *
* PO-box 80043 * LLL AA AA *
* Zip: 3508 TA * LLLLLAAALLLAAALLL *
* Utrecht The Netherlands * LLLLLAAALLLAAALLLL *
* Tel: 030 - 538911 * AAA AAA *
* Fax: 030 - 539036 * AAA AAA *
***************************************************************************




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sun, 23 Apr 1995 7:37:35 -0500 (CDT)
Subject: Millipore filters

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From: W.L. Steffens :      STEFFENS.B-at-calc.vet.uga.edu
Date: Fri, 21 Apr 1995 11:01:14 EST
Subject: Millipore filters

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To Thor Bostrom,

Be very certain that your Millipore filters are of the polycarbonate type
and NOT nitrocellulose, as these will not survive the CPD. The problem
with the polycarbonate filters though, is that they tend to roll up into
tight tubes when dried. To hold them in place during CPD, I cut them into
grid-sized pieces and clamp them between a 50 mesh folding (oyster) grid.
Several of these can then be mounted on a single stub.

Hope this helps.

-=W.L. Steffens=-
College of Veterinary Medicine
University of Georgia




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sun, 23 Apr 1995 7:41:50 -0500 (CDT)
Subject: Re: CPD of millipore filters

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From: John Warrack-1 :      John_Warrack-1%notes-at-sb.com
Date: 21 Apr 95 17:52:37 EDT
Subject: Re: CPD of millipore filters

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Message-Id: {9504211955.AA2171-at-pho018.sb.com}
To: MICROSCOPYLISTZALUZEC {MICROSCOPYLISTZALUZEC-at-AAEM.AMC.ANL.GOV}

Millipore filters can be successfully CPD'd in envelopes made from folded
filter paper, closed with a staple at the open edge. I use Whatman 541 4.25cm
papers routinely with 12mm filters. The sample code can be written on the paper
with pencil, and this mark can then be used to determine the "up" side of the
millipore filter - sometimes the sample is invisible to the eye.

John_Warrack-1%notes-at-sb.com

SmithKline Beecham UK






From: ANLAEM::ZALUZEC Nestor J. Zaluzec-Argonne Nat. Lab. 21-APR-1995 13:45:56.22
Date: Sun, 23 Apr 1995 7:43:20 -0500 (CDT)
Subject: Need virus Pictures

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From: Joyce Craig :      bafpjec-at-uxa.ecn.bgu.edu
Date: Fri, 21 Apr 1995 09:49:39 -0500 (CDT)
Subject: virus

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We have 50 fifth graders from Hammond Indiana coming to visit in May.
They have been in a special science program and all want to see viruses
in the TEM. Unfortunately, I have never worked with viruses. Does
anyone have any ideas I could use? I have a JEOL 1200 TEMSCAN
which is capable of visualizing them, but have to do a prep of some
sort. Fortunately I have a CRT & also a computer with a program so they
can see on either CRT & don't have to rely on the green screen.
Thanks from Joyce at Chicago State University.
.





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sun, 23 Apr 1995 7:44:12 -0500 (CDT)
Subject: Polishing Sapphire

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From: South Bay Technology :      73531.1344-at-compuserve.com
Date: 21 Apr 95 17:51:45 EDT
Subject: Polishing Sapphire

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I posted a message earlier for information on polishing sapphire for TEM. I'd
now like to expand that to get any information at all on polishing sapphire.
Ideal information would be suggested sample load, wheel speed, abrasive type
etc. I've received several bits of information - naturally, it all conflicts to
some degree. Perhaps if I get enough information, I can make a reasonable
conclusion.

Thanks for your help!

David Henriks TEL: 800-728-2233
South Bay Technology, Inc. 714-492-2600
1120 Via Callejon FAX: 714-492-1499
San Clemente, CA 92673 e-mail: 75431.1344-at-compuserve.com






From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sun, 23 Apr 1995 7:40:16 -0500 (CDT)
Subject: info on EM Salaries

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From: Michael Cammer :      cammer-at-aecom.yu.edu
Date: Fri, 21 Apr 1995 11:04:41 -0400 (EDT)
Subject: info on EM Salaries

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I run the day to day operations of the confocal microscopy/ video
microscopy/ computer image analysis lab. I am the lowest rank faculty.
My salary began at $26.5k in 1992 and is up to $32.5 now. To keep the
Facility going, I work, on average, 9 hour days. We are in New York City.
My salary feels low for the area. In another part of the country, this
might be more comfortable.

You could try writing to macaluso-at-aecom.yu.edu to find out about the EM
facility here.

Please do not post on the bboard.

-----------------------------------------------
} } I'm trying to get some information on salary ranges
for } } electron microscopists and EM core facility managers. Is there a
} } source I can check? Thank you.







From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sun, 23 Apr 1995 7:42:32 -0500 (CDT)
Subject: Re: CPD of millipore filters

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From: chandler-at-lamar.ColoState.EDU (John Chandler)
Date: Fri, 21 Apr 1995 12:08:02 -0700
Subject: Re: CPD of millipore filters

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Message-Id: {v01510102abbdaacb97c6-at-[129.82.126.28]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

} Millipore filters can be successfully CPD'd in envelopes made from folded
} filter paper, closed with a staple at the open edge. I use Whatman 541 4.25cm
} papers routinely with 12mm filters. The sample code can be written on the
} paper
} with pencil, and this mark can then be used to determine the "up" side of the
} millipore filter - sometimes the sample is invisible to the eye.
}
} John_Warrack-1%notes-at-sb.com
}
} SmithKline Beecham UK

One way we handle specimens like this for CPD is to sandwich them between
two ring magnets. The thickness of the magnets protects the specimen.

The magnets are 1/2 inch diameter, 1/8 inch thick, with a 1/4 inch center
hole. They are in the Edmond Scientific catalog as stock number D41,990.
A pack of 25 was $5 in 1993.

Hope this helps.


John
chandler-at-lamar.ColoState.EDU
http://www.vetmed.colostate.edu/anatomy/faculty/chandler.html






From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sun, 23 Apr 1995 7:43:38 -0500 (CDT)
Subject: Alternates to D-19

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From: fskarl-at-goodyear.com (Frank Karl)
Date: Fri, 21 Apr 1995 15:01:27 -0500
Subject: Alternates to D-19

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X-Sender: t456b15-at-rds163
Message-Id: {v01510102abbdbeec0db8-at-[163.243.13.93]}
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Hi everyone!

We are currently using Kodak D-19 developer and Kodak Electron Microscopy
Film 4489. We typically develop 123 pieces of film before changing
developer and due to the relatively large number of potential users (6
people) we burn through the stock solution of D-19. Mixing D-19 from
powder is time consuming activity, and in this era of doing more with less
I would like to find a developer sold as a liquid concentrate. This would
speed up darkroom maintenance and let us spend more time developing and
less time preparing to develop.

Can anyone recommend a liquid developer? And while I'm asking, is there a
better or equivalent film than 4489?


Thanks for your input.


Frank Karl fskarl-at-goodyear.com
Goodyear Tire & Rubber Co. Voice 216.796.7818
Analytical Services - Dept 415B Fax 216.796.3304
142 Goodyear Blvd
Akron, OH 44305
U.S.A.









From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sun, 23 Apr 1995 7:44:44 -0500 (CDT)
Subject: Re: CPD of millipore filters

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From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sun, 23 Apr 1995 7:45:16 -0500 (CDT)
Subject: Re: CPD of millipore filters

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From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sat, 22 Apr 1995 9:49:05 -0500 (CDT)
Subject: CPD of Millipore membrane filters

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From: GVKM07A-at-prodigy.com (DR CHARLES A GARBER)
Date: Fri, 21 Apr 1995 02:35:23 EDT
Subject: CPD of Millipore membrane filters

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This is in response to posting from Dr. Thor Bostrom on April 20:

There is an easy solution:

We have been making for some years a product called "Microporous
Specimen Capsules". The original product had a pore size range of 120-
200 um, and is our part SPI #13215 and has overall dimensions of 10 mm
x 10 mm. However, you will have to cut out a small piece of the
membrane filter prior to insertion of the piece into the capsule.

We have a second product, exactly like the first, but with a pore size
of 78um which should be used if the features on the membrane at that
small.

We expect to have available yet a third product, just like the other
two, but with pore size on the order of 30 um.

Charles A. Garber
PRESIDENT
SPI SUPPLIES
PO BOX 656
WEST CHESTER, PA 19380

Ph: (610) 436-5400
(800) 2424-SPI

FAX:(610) 436-5755

e-mail: GVKM07A-at-prodigy.com





From: ANLAEM::ZALUZEC Nestor J. Zaluzec-Argonne Nat. Lab. 23-APR-1995 07:35:56.63
Date: Sun, 23 Apr 1995 7:38:30 -0500 (CDT)
Subject: Re: osmium tetroxide oxidation

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From: gwerdos-at-gnv.ifas.ufl.edu (Greg Erdos)
Date: Fri, 21 Apr 1995 08:43:21 -0500 (EST)
Subject: Re: osmium tetroxide oxidation

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From: gwerdos-at-gnv.ifas.ufl.edu (Greg Erdos)
Date: Fri, 21 Apr 1995 08:43:21 -0500 (EST)
Subject: Re: osmium tetroxide oxidation

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I would like to have this information please.
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Greg Erdos Phone: 904-392-1295
Scientific Director, ICBR EMCL
218 Carr Hall Fax 904-846-0251
University of Florida E-mail: gwerdos-at-gnv.ifas.ufl.edu
Gainesville, FL 32611





From: ANLAEM::ZALUZEC Nestor J. Zaluzec-Argonne Nat. Lab. 23-APR-1995 07:36:16.25
Date: Sun, 23 Apr 1995 7:39:03 -0500 (CDT)
Subject: Re: CPD of millipore filters

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From: gwerdos-at-gnv.ifas.ufl.edu (Greg Erdos)
Date: Fri, 21 Apr 1995 08:47:24 -0500 (EST)
Subject: Re: CPD of millipore filters

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From: gwerdos-at-gnv.ifas.ufl.edu (Greg Erdos)
Date: Fri, 21 Apr 1995 08:47:24 -0500 (EST)
Subject: Re: CPD of millipore filters

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What we have done is to put two filter face to face and hold them
together using the cap of a BEEM capsule that has had a large hole punched
in it with a cork borer and a ring cut from the top of the capsule that will
fit inside the cap.

SO it goes cap, then two filters, then the ring on top and push
them together. Something like an embroidery hoop/
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Greg Erdos Phone: 904-392-1295
Scientific Director, ICBR EMCL
218 Carr Hall Fax 904-846-0251
University of Florida E-mail: gwerdos-at-gnv.ifas.ufl.edu
Gainesville, FL 32611





From: ANLAEM::ZALUZEC Nestor J. Zaluzec-Argonne Nat. Lab. 23-APR-1995 07:36:35.03
Date: Sun, 23 Apr 1995 7:39:27 -0500 (CDT)
Subject: Re: CPD of millipore filters

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From: sfzane-at-unccvm.uncc.edu (Sandra F. Zane)
Date: Fri, 21 Apr 1995 10:52:47 -0400
Subject: Re: CPD of millipore filters

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Good Morning Dr. Bostrom,
In reply to your question about handling millipore filters containing
bacteria and zooplankton for SEM, we do this sort of thing by covering the
filter containing the sample with a spacer (which comes packaged between the
filters) and sandwich that between two washers which are held together with
small alligator clamps. Be sure when you purchase the washers that they are
made of a material which will not rust. I'm not sure what the ones I use
are made of....perhaps stainless or aluminum. I took them to a trophy shop
and had numbers engraved on them to help with sample identification.
I hope this is helpful.
Sandra Zane






From: sfzane-at-unccvm.uncc.edu (Sandra F. Zane)
Date: Fri, 21 Apr 1995 10:52:47 -0400
Subject: Re: CPD of millipore filters

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Sandra F. Zane, EM Tech.
Biol. Dept. UNCC
Charlotte, NC 28223
sfzane-at-unccvm.uncc.edu
Fax (704) 547-3128





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sun, 23 Apr 1995 15:05:08 -0500 (CDT)
Subject: Loctite 460 super glue

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From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sun, 23 Apr 1995 15:09:48 -0500 (CDT)
Subject: Curling of membrane filters

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From: GVKM07A-at-prodigy.com (DR CHARLES A GARBER)
Date: Sun, 23 Apr 1995 15:26:43 EDT
Subject: Curling of membrane filters

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W.L. Steffens and Thor Bostrom:

If the curling of membrane filters is a problem, then try using our
silver membrane filter product, exactly like the polymer membrane (e.g.
MCE) product in structure but made out of pure silver. Any curling is
very slight and the bonus is that what ever conductive layer is
eventually applied can be much less (maybe no conductive coating at all
in fact) because of the inherent conductivity of the substrate. The
aluminum oxide membrane filters are even stiffer, but are not
conductive, but the pores are the smallest available in any membrane
filter product (e.g. 20 nm). These are on pages 53 and 54 of our SPI
Supplies 1991 SourceBook (catalog) of products for the EM laboratory.
E-mail me if you need a copy.

Charles A. Garber
PRESIDENT
SPI SUPPLIES
PO BOX 656
WEST CHESTER, PA 19380

Ph: (800) 2424-SPI
FAX:(610) 436-5755

e-mail: GVKM07A-at-prodigy.com
or
sp-supp-at-cerf.com [April 25 to May 3 when I am abroad]





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sun, 23 Apr 1995 15:10:44 -0500 (CDT)
Subject: Re: grey levels - film vs digital

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From: em-at-mediacity.com (E. Monberg)
Date: Sun, 23 Apr 1995 13:20:25 -0800
Subject: grey levels - film vs digital

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Message-Id: {m0s385J-000rc2C-at-easynet.com}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"


= 8 x 10E3 THAT is a HOT CCD !

I seem to recollect this as a kind of wondrous
upper limit, How many charges
fit in a charge bucket vs the noise floor?
Is the underlying question.

Candlelight to direct sun ratio is about 10E6

} each pixel of
} film can capture only a few gray levels
} (roughly equivalent to the number of silver grains

THE GRAINS ARE NOT ALL THE SAME SIZE - - -

} that can fit into the area of one pixel without
} overlapping)". Buonaquisti (Microscopy Today 95(1):12-13, 1995) states
} film "is an anlog process and, as such, one can argue that sheet film has
} an infinite gray scale resolution". Can anyone comment on this? I have
} always thought more along Krivanek and Mooney's reasoning. Does anyone
} know the number of grey levels film can capture? Thanks for any comments.
}
}
} Thomas E. Phillips, Ph.D.
} Associate Professor of Biological Sciences
} Director, Molecular Cytology Core Facility
} 3 Tucker Hall
} University of Missouri
} Columbia, MO 65211
} (314)-882-4712 (voice)
} (314)-882-0123 (fax)




Regards,

Ed M.

Ed Monberg, General Manager
Laser Motion and Development Co.
3101 Whipple Road
Union City, CA 94587-1216
510-429-1060, FAX 429-1065
em-at-mediacity.com






From: Michael Cammer :      cammer-at-aecom.yu.edu
Date: Sun, 23 Apr 1995 22:12:03 -0400 (EDT)
Subject: Re: info on EM Salaries

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On Sun, 23 Apr 1995, Nestor J. Zaluzec-Argonne Nat. Lab. wrote:

} From: SMTP%"cammer-at-aecom.yu.edu" 21-APR-1995 09:19:28.20
} To: ZALUZEC
} CC:
} Subj: info on EM Salaries
}
} Date: Fri, 21 Apr 1995 11:04:41 -0400 (EDT)
} From: Michael Cammer {cammer-at-aecom.yu.edu}
} Subject: info on EM Salaries
} To: delannoy-at-welchlink.welch.jhu.edu
} Cc: ZALUZEC-at-AAEM.AMC.ANL.GOV
} In-Reply-To: {950420095748.1cc-at-AAEM.AMC.ANL.GOV}
} Message-Id: {Pine.3.07.9504211140.A20241-a100000-at-alsys1}
} Mime-Version: 1.0
} Content-Type: TEXT/PLAIN; charset=US-ASCII
}
} To keep the
} Facility going, I work, on average, 9 hour days.

As I sit here on a Sunday night noticing that my reply was posted here, I
would like to clarify or revise. Nine hour days was a glib comment. This
means coming in at 9:30 and leaving at 7:00 with a break merely for lunch
if nobody walks in demanding help. And this does not include
administrative paperwork which must be prepared on weekend evenings when
interruptions are less likely. I'm not complaining and, in fact, I love
being constantly challenged with interesting projects; I'm merely
clarifying the pay scale.


} -----------------------------------------------
} } } I'm trying to get some information on salary ranges
} for } } electron microscopists and EM core facility managers. Is there a
} } } source I can check? Thank you.







From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Mon, 24 Apr 1995 8:45:57 -0500 (CDT)
Subject: EM Salaries....

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From: Michael Cammer :      cammer-at-aecom.yu.edu
Date: Sun, 23 Apr 1995 22:12:03 -0400 (EDT)
Subject: Re: info on EM Salaries

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On Sun, 23 Apr 1995, Nestor J. Zaluzec-Argonne Nat. Lab. wrote:

} From: SMTP%"cammer-at-aecom.yu.edu" 21-APR-1995 09:19:28.20
} To: ZALUZEC
} CC:
} Subj: info on EM Salaries
}
} Date: Fri, 21 Apr 1995 11:04:41 -0400 (EDT)
} From: Michael Cammer {cammer-at-aecom.yu.edu}
} Subject: info on EM Salaries
} To: delannoy-at-welchlink.welch.jhu.edu
} Cc: ZALUZEC-at-AAEM.AMC.ANL.GOV
} In-Reply-To: {950420095748.1cc-at-AAEM.AMC.ANL.GOV}
} Message-Id: {Pine.3.07.9504211140.A20241-a100000-at-alsys1}
} Mime-Version: 1.0
} Content-Type: TEXT/PLAIN; charset=US-ASCII
}
} To keep the
} Facility going, I work, on average, 9 hour days.

As I sit here on a Sunday night noticing that my reply was posted here, I
would like to clarify or revise. Nine hour days was a glib comment. This
means coming in at 9:30 and leaving at 7:00 with a break merely for lunch
if nobody walks in demanding help. And this does not include
administrative paperwork which must be prepared on weekend evenings when
interruptions are less likely. I'm not complaining and, in fact, I love
being constantly challenged with interesting projects; I'm merely
clarifying the pay scale.


} -----------------------------------------------
} } } I'm trying to get some information on salary ranges
} for } } electron microscopists and EM core facility managers. Is there a
} } } source I can check? Thank you.







From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Mon, 24 Apr 1995 8:58:18 -0500 (CDT)
Subject: RE: Alternates to D-19

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From: bob-at-befvax.uchicago.edu
Date: Sun, 23 Apr 1995 16:32:51 EDT
Subject: RE: Alternates to D-19

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How about making up a LARGE volume of D19 (say 10 gallons or more) and storing
them in brown bottles. It will be cheaper than buying liquid developer
and the D19 will last a long time if the bottles are filled and capped.


Bob Josephs
Univ of Chicago

312 702 1077





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Mon, 24 Apr 1995 9:06:28 -0500 (CDT)
Subject: osmium update

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From: SGKCCK-at-aol.com
Date: Mon, 24 Apr 1995 05:00:33 -0400
Subject: osmium update

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Dear Ed,
Here is The infor you requested. If you can not find it let me know for I
will send you a copy: To all others that have requested a copy they have all
been send and posted today.
50th anniversary Meeting of EMSA proceedings, Boston.
Author: W.H. Muss
Let me know if you need anything else.
Sincerely
Stacie Kirsch
Electron Microscopy Sciences




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Mon, 24 Apr 1995 9:07:19 -0500 (CDT)
Subject: CPD of millipore filters

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From: Microbill-at-aol.com
Date: Mon, 24 Apr 1995 06:59:06 -0400
Subject: Re: CPD of millipore filters

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I used to CPD millipore filters with diatoms and found that there was little
loss if the filters were simply a sandwich of two filters. The filters
become very brittle after CPDing. If you decide to freeze dry the samples be
sure to so from water free of buffers or salt or you will find all sorts of
strange crystals.
Good Luck.




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Mon, 24 Apr 1995 9:01:51 -0500 (CDT)
Subject: Looking for a SEM atlas in biomedicine

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From: Bilge Hakan Sen :      SEN-at-dishekimligi.ege.edu.tr
Date: Mon, 24 Apr 1995 10:08:32 TUR+2
Subject: Looking for a SEM atlas in biomedicine

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Anybody knows a SEM book related to techniques and pictures related
to dentistry and medicine?

______________________________
Bilge Hakan SEN, DDS, PhD
Ege Universitesi
Dishekimligi Fak.
Bornova/Izmir/Turkiye 35100

Tel. 90 232 3880328 (Work)
90 232 2390467 (Home)
Fax. 90 232 3880325




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Mon, 24 Apr 1995 9:07:44 -0500 (CDT)
Subject: dissolving solvent?

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From: George.C.Ruben-at-Dartmouth.EDU (George C. Ruben)
Date: 24 Apr 95 07:26:41 EDT
Subject: dissolving solvent?

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Message-id: {16166369-at-donner.Dartmouth.EDU}


Ron,

"We haven't tried that particular brand but
cyanoacrylates (superglues)
shouldn't be used where they can be illuminated by the electron beam
in our experience. They tend to bubble and release sludge into the
column when not cured rock hard. M-Bond 610, Gatan G-1, Epotec 353ND,
and Devcon-2-ton epoxys are better choices.

Superglues are far better than wax for mounting parts on preparation
fixtures. They dissolve in solvents nearly as fast as wax but without
a waxy residue."

What solvent do you use to dissolve and remove super glue?

Thanks,
George C. Ruben
Dept Biological Sciences
Dartmouth College
Hanover, NH 03755
tel 603 646-2144
fax 603 646-1347




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Mon, 24 Apr 1995 9:08:07 -0500 (CDT)
Subject: D of millipore filters -REPONSE to Dr thor bostrom

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From: Diane Montpetit :      montpetitd-at-EM.AGR.CA
Date: Sun, 23 Apr 1995 07:25:46 -0400
Subject: CPD of millipore filters -REPONSE to Dr thor bostrom

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Message-Id: {sf9b52d5.053-at-EM.AGR.CA}
X-Mailer: Novell GroupWise 4.1

I routinely CPD bacteria on a millipore filter that I roll like a "hand made"
cigarette, the specimens lying inside the roll, and I simply put that roll in
the metal mesh basket of the CPD, it works very well and i do not loose
material.

Diane Montpetit
Food research center
St-Hyacinthe, Quebec, Canada
tel: 514 773 1105
fax: 514 773 8461






From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Mon, 24 Apr 1995 12:12:42 -0500 (CDT)
Subject: Alternates to D-19

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From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Mon, 24 Apr 1995 12:16:06 -0500 (CDT)
Subject: TEM: LocTite 460 super glue

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Message-Id: {Chameleon.950424115119.tonygr-at-emlab.mit.edu}




From: Brian G. Demczyk :      demczyk-at-ERXINDY.rl.plh.af.mil
Date: Mon, 24 Apr 1995 13:05:03 +0059 (EDT)
Subject: Re: TEM: LocTite 460 super glue

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I meant that it decomposes under the beam, releasing unwanted
hydrocarbons into the column. I generally use M-Bond 610 for
making cross sections, as it doesn't have this problem-also,
it won't dissolve in acetone like Epoxy resins will.






From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Mon, 24 Apr 1995 12:16:41 -0500 (CDT)
Subject: alternatives to d-19

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From: Brian G. Demczyk :      demczyk-at-ERXINDY.rl.plh.af.mil
Date: Mon, 24 Apr 1995 13:12:11 +0059 (EDT)
Subject: Re: Alternates to D-19

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We use 4489 for routine work and S0163 for high resolution (or low
dosage work). The SO163 has a larger grain, but is higher sensitivity that
4489.





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Mon, 24 Apr 1995 9:05:01 -0500 (CDT)
Subject: Alternatives to D-19

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From: Dr R.J. Keyse :      keyse-at-liverpool.ac.uk
Date: Mon, 24 Apr 1995 09:25:35 +0100 (BST)
Subject: Re: Alternates to D-19

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} We are currently using Kodak D-19 developer and Kodak Electron Microscopy
} Film 4489. We typically develop 123 pieces of film before changing
} developer and due to the relatively large number of potential users (6
} people) we burn through the stock solution of D-19. Mixing D-19 from
} powder is time consuming activity, and in this era of doing more with less
} I would like to find a developer sold as a liquid concentrate. This would
} speed up darkroom maintenance and let us spend more time developing and
} less time preparing to develop.
}
} Can anyone recommend a liquid developer? And while I'm asking, is there a
} better or equivalent film than 4489?

I think you'll find that you can use D-19 for up to about 800 plates developed
without any side effects, assuming you have a nitrogen gas burst system.
SO-163 is the film of choice. Its faster than 4489.
Rob Keyse




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Mon, 24 Apr 1995 9:14:58 -0500 (CDT)
Subject: SuperGlue

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From: _ _ _ _ _ MARK W. LUND _ _ _ _ _ :      LUNDM-at-PHYSC2.BYU.EDU
Date: Mon, 24 Apr 1995 08:47 MDT
Subject: Super glue in vacuum?

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Hi,
I was a lens-designer and all-around optical engineer in one of my
previous lives. A few times we had problems with contamination
on lenses in various military instruments that we made. The problem
was a cheesy goo (when examined over the microscope) and was always
solved by me walking around the production/assembly floor and looking
for the super-glue. Often a tech or assembler would bring in a tube
in order to stake wires or subassemblies to make the work easier.
When the instrument was later evacuated for back-filling with inert
gas the glue would out-gas and spray all this goo around.
I did a couple of experiments in a vacuum oven to verify that this
is what we saw.

Now, I have to admit that nothing was done to 'cure' this super-glue,
it may be that the heating was necessary (during the pump-down
there was a bake-out to try to eliminate water vapor) to cause the
out-gassing, but I am sure that NASA won't let super-glued components
into space.

My $0.02
best regards
mark

Mark W. Lund, PhD
Director
MOXTEK, Inc.
Orem UT




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Mon, 24 Apr 1995 8:59:54 -0500 (CDT)
Subject: e: cryo-ultramicrotomy of macrophages

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From: Gerry LITTLE :      ANGJL-at-medicine.newcastle.edu.au
Date: Mon, 24 Apr 1995 08:18:22 GMT +11
Subject: Re: cryo-ultramicrotomy of macrophages

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}
} Subj: cryo-ultramicrotomy of macrophages
}

} Hello. I am currently having difficulty getting cryo sections of mouse bone
} marrow macrophages to stick on grids.
}
} I am using conventional techniques with a dry diamond knife, picking the sections
up with sucrose and floating the grids on buffer before further prossing
} with immunogold. I have been using this method for some time with different
} types of cells and intracellular parisites with no difficulty. My problem
} seems to be with just this one cell type...
}
} Any advise from someone in the know?
}
} Thank you.
}
} Jaime A. Dant
} Washington University School of Medicine
} St. Louis
} jaime-at-borcim.wustl.edu

G'day Jaime,
I haven't tried this type of tissue, we work with peripheral nerve.
One technique that we have found useful for some nerve types is the
electrostatic transfer method. The procedure was published by Tsuji
et al. 1992, in the Arch. Histol. Cytol. vol 55, p. 423-428.
Hope this helps,
Regards,
Gerald Little.

Dr Gerald J. Little | Ph (61 49) 215618
The Neuroscience Group |
Discipline of Anatomy | Fax (61 49) 216903
Faculty of Medicine and |
Health Sciences |
The University of Newcastle | Email ANGJL-at-medicine.newcastle.edu.au
Australia, 2308 |




From: Fred Pearson :      eoptics-at-mcmail.CIS.McMaster.CA
Date: Mon, 24 Apr 1995 14:34:43 +0059 (EDT)
Subject: Re: Alternates to D-19

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}
} We are currently using Kodak D-19 developer and Kodak Electron Microscopy
} Film 4489. We typically develop 123 pieces of film before changing
} developer and due to the relatively large number of potential users (6
} people) we burn through the stock solution of D-19. Mixing D-19 from
} powder is time consuming activity, and in this era of doing more with less
} I would like to find a developer sold as a liquid concentrate. This would
} speed up darkroom maintenance and let us spend more time developing and
} less time preparing to develop.
}
} Can anyone recommend a liquid developer? And while I'm asking, is there a
} better or equivalent film than 4489?

} Frank Karl fskarl-at-goodyear.com
} Goodyear Tire & Rubber Co. Voice 216.796.7818
} Analytical Services - Dept 415B Fax 216.796.3304
} 142 Goodyear Blvd
} Akron, OH 44305
} U.S.A.

Frank:

Try making up batches of 5 gal. or more in large cube-tainers, and then
mix D19 1:2 parts H20. We develop our negs. (Kodak SO-163) for 4 min. The
S0-163 film can be pushed for longer development times if necessary, up
to 1\2 hr. for some special experiments.

Fred




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Mon, 24 Apr 1995 19:51:28 -0500 (CDT)
Subject: Re: Alternates to D-19

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From: Brian G. Demczyk :      demczyk-at-ERXINDY.rl.plh.af.mil
Date: Mon, 24 Apr 1995 13:10:25 +0059 (EDT)
Subject: Re: Alternates to D-19

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We typically dilute the D-19 mixed stock with water 1:1 and develope
700 plates between changings. I've never seen any degradation of the
developed negatives up to that time and one may even be able to go longer
between developer changes-in fact, we've gone over 1000 on several
occasions when people were too lazy to make up new developer.






From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Mon, 24 Apr 1995 19:51:58 -0500 (CDT)
Subject: Re: Looking for a SEM atlas in biomedicine

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From: Marcelle A Gillott :      magem-at-csd.uwm.edu
Date: Mon, 24 Apr 1995 15:39:14 -0500 (CDT)
Subject: Re: Looking for a SEM atlas in biomedicine

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Dont know if it is still in print (pub in 79) but there is a nice SEM
atalas from The Freman Publ Co (the ones who do Scientific American) and
As I seem to recall, it was relatively inexpensive:

Tisssues & Organs: a text atlas of scanning electron microscopy

RG Kessel & RH Kardon

Happy hunting

Marcelle Gillott
UWM





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Mon, 24 Apr 1995 19:52:43 -0500 (CDT)
Subject: Re: greys levels, film vs digital

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From: bergrh-at-cc.memphis.edu (R. Howard Berg)
Date: Mon, 24 Apr 1995 12:27:49 +0600
Subject: Re: greys levels, film vs digital

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Thomas E. Phillips wants comments on grey levels possible in film vs.
digital. Film response is a sigmoidal curve and one adjusts exposure to
fit the light coming off the subject to the linear portion of the curve in
the film's response. Black & white film provides a greater number of lens
aperture stops (6-8) to capture greys compared to color film (about 4,
depends on the film). So the answer to the question of grey values
possible in film is that one can capture a great variety of greys in film,
but not all in one image-only a relatively narrow range can be captured,
with the appropriate adjustment of exposure. CCDs have a linear response
over several orders of magnitude. Slow scan CCDs can capture a remarkable
range of greys in each image, in which case one can choose which range of
greys to display on the moniter.




R. Howard Berg, Ph.D.
Biology Department
University of Memphis
Memphis, TN, 38152
E mail: bergrh-at-cc.memphis.edu
phone: 901-678-4449 fax: 901-678-4457







From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Mon, 24 Apr 1995 19:53:09 -0500 (CDT)
Subject: Making Holey Carbon Films

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From: bozzola-at-siu.edu (John. J. Bozzola)
Date: Mon, 24 Apr 1995 18:51:07 -0600
Subject: Making Holey Carbon Films

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Anyone have a good, reproduceable protocol for making holey carbon films
for high resolution work? We have tried numerous methods including
Formvar/glycerol mixtures, steaming, followed by opening of pseudoholes
with solvents and carbonization of the film. Major problem has been
obtaining sufficient numbers of holes (either too many or not enough are
obtained using glycerol). Another problem seems to be getting the Formvar
to separate from the slide. Evaporated Victawet helps, but this seems a bit
constraining. Thanks mucho.

John J. Bozzola
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
Phone: 618-453-3730
Fax: -2665
Email: bozzola-at-siu.edu OR bozzola-at-qm.c-cem.siu.edu






From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Mon, 24 Apr 1995 19:53:46 -0500 (CDT)
Subject: Alternatives to D-19

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From: Fred Pearson :      eoptics-at-mcmail.CIS.McMaster.CA
Date: Mon, 24 Apr 1995 14:34:43 +0059 (EDT)
Subject: Re: Alternates to D-19

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}
} We are currently using Kodak D-19 developer and Kodak Electron Microscopy
} Film 4489. We typically develop 123 pieces of film before changing
} developer and due to the relatively large number of potential users (6
} people) we burn through the stock solution of D-19. Mixing D-19 from
} powder is time consuming activity, and in this era of doing more with less
} I would like to find a developer sold as a liquid concentrate. This would
} speed up darkroom maintenance and let us spend more time developing and
} less time preparing to develop.
}
} Can anyone recommend a liquid developer? And while I'm asking, is there a
} better or equivalent film than 4489?

} Frank Karl fskarl-at-goodyear.com
} Goodyear Tire & Rubber Co. Voice 216.796.7818
} Analytical Services - Dept 415B Fax 216.796.3304
} 142 Goodyear Blvd
} Akron, OH 44305
} U.S.A.

Frank:

Try making up batches of 5 gal. or more in large cube-tainers, and then
mix D19 1:2 parts H20. We develop our negs. (Kodak SO-163) for 4 min. The
S0-163 film can be pushed for longer development times if necessary, up
to 1\2 hr. for some special experiments.

Fred




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Mon, 24 Apr 1995 9:00:31 -0500 (CDT)
Subject: Alternatives to d-19

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From: SALLY STOWE :      STOWE-at-rsbs-central.anu.edu.au
Date: Mon, 24 Apr 1995 08:53:11 EST10
Subject: Re: Alternates to D-19

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} From: fskarl-at-goodyear.com (Frank Karl)
} Subject: Alternates to D-19
}
} Hi everyone!
}
} We are currently using Kodak D-19 developer and Kodak Electron Microscopy
} Film 4489. We typically develop 123 pieces of film before changing
} developer and due to the relatively large number of potential users (6
} people) we burn through the stock solution of D-19. Mixing D-19 from
} powder is time consuming activity, and in this era of doing more with less
} I would like to find a developer sold as a liquid concentrate. This would
} speed up darkroom maintenance and let us spend more time developing and
} less time preparing to develop.
}
} Can anyone recommend a liquid developer? And while I'm asking, is there a
} better or equivalent film than 4489?
}
}
} Thanks for your input.
}
}
} Frank Karl fskarl-at-goodyear.com
} Goodyear Tire & Rubber Co. Voice 216.796.7818
} Analytical Services - Dept 415B Fax 216.796.3304
} 142 Goodyear Blvd
} Akron, OH 44305
} U.S.A.
}
} We use Ilford PQ Universal developer with Kodak 4489 film, for the
convenience of using a liquid developer. Over the years we have
tried different strengths, and are currently using a 1:9 dilution,
as per the standard instructions for film. We normally use a fresh
solution every day. The mismatch came about
because we originally used Ilford film, and when switching to Kodak
it was natural to test with the same processing chemicals. - the
results were fine. However if there is a real incompatibility
lurking in the background, we would be interested to know!

} best wishes,
Sally Stowe

}
}
----------------------------------------------------------------------
Sally Stowe Australian National Univ.
Facility Coordinator Canberra, AUSTRALIA
ANU Electron Microscopy Unit Ph 61 6 249 2743
RSBS, Box 475
Email stowe-at-rsbs-central.anu.edu.au FAX 61 6 249 4891
------------------------------------- --------------------------------
-





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Mon, 24 Apr 1995 9:13:52 -0500 (CDT)
Subject: Re: Need virus Pictures

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From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Tue, 25 Apr 1995 12:21:52 -0500 (CDT)
Subject: grey levels - film vs digital

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From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Tue, 25 Apr 1995 12:22:45 -0500 (CDT)
Subject: Separating Formvar from Slides

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From: gburges-at-cc.UManitoba.CA (Garry Burgess)
Date: Mon, 24 Apr 1995 22:43:59 -0500
Subject: Separating Formvar from Slides

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Another problem seems to be getting the Formvar
} to separate from the slide.

} John J. Bozzola
} Center for Electron Microscopy
} Southern Illinois University
} Carbondale, IL 62901-4402
} Phone: 618-453-3730
} Fax: -2665
} Email: bozzola-at-siu.edu OR bozzola-at-qm.c-cem.siu.edu

To separate the formvar from the slide, I take a razor blade, and holding
it at a 45 degree angle relative to the edge of the slide, I scrape all 3
sides of the slide, then I cut a line several time near the top of the
slide, so that all sides have been cut with the razor blade. This should
really help separate the formvar from the slide.

Garry Burgess






From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Tue, 25 Apr 1995 12:28:45 -0500 (CDT)
Subject: GLUES

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From: Stephan Coetzee :      STEPHAN-at-gecko.biol.wits.ac.za
Date: Tue, 25 Apr 1995 08:22:50 GMT+2
Subject: GLUE

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Dear Microscopists

I see that we are back on the subject of glues again. There are two
publications by M J Witcomb (The suitability of various adhesives and
mounting media for scanning electron microscopy :Journal of
Microscopy Vol 121 March 1981 pp. 289-308;
The suitability of various mounting media for scanning electron
microscopy. II. General glues Journal of Microscopy, Vol 139 Pt 1,
July 1985 pp 75-114)

I think this may clear things up a bit and set a standard of criteria
for glues.



Stephan H Coetee
Electron Microscope Unit
Private Bag 3
Wits
2050 Stephan-at-Gecko.biol.WITS.ac.za

Tell: (011) 716 2419
Fax : (011) 339 3407




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Tue, 25 Apr 1995 12:30:49 -0500 (CDT)
Subject: Making Holey Carbon Films

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From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Tue, 25 Apr 1995 12:37:55 -0500 (CDT)
Subject: D-19 Alternative

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From: Doug Davis :      doug_davis-at-maillink.berkeley.edu
Date: 25 Apr 1995 09:10:30 -0700
Subject: Re: D-19.alt

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Message-ID: {n1413321423.31186-at-maillink.berkeley.edu}

Subject: Time: 8:00 AM
OFFICE MEMO RE} D-19.alt Date: 4/25/95

We use Kodak HRP (high resolution plate) developer, Kodak catalog # 140-1306
with 4489 film. Kodak EIFilm SO-163 is a reasonable alternative and is more
sensitive than 4489, reducing the time required for exposure, and is also a
fine grain emulsion.
Kodak has a tech sheet on this; call 1-800-242-2424, ext. 50 for technical
info. I think it is publication # JJ-7

doug_davis -at-maillink.berkeley.edu





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Tue, 25 Apr 1995 12:41:21 -0500 (CDT)
Subject: Another old TEM available

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From: Doug Davis :      doug_davis-at-maillink.berkeley.edu
Date: 25 Apr 1995 10:23:29 -0700
Subject: Another old TEM available

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Message-ID: {n1413317002.94149-at-maillink.berkeley.edu}

Subject: Time: 7:57 AM
OFFICE MEMO Another old TEM available Date: 4/17/95

Philips 200 at UC Berkeley, has not run in two years.

Contact: Prof. Ed Sylvester at (510) 642-7353 or Ethel Nakamura in Wellman
Hall at (510) 642-1603 for details.
Some funds available for moving, Ask Ed.





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Tue, 25 Apr 1995 12:31:35 -0500 (CDT)
Subject: virus pictures

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From: EMLAB-at-opus.mco.edu
Date: Tue, 25 Apr 1995 09:07:56 -0500 (EST)
Subject: virus pictures

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Dear Joyce,

In response to your query, a very good source of viruses are your local
neighbor's baby's. Ask your friends if one of their children have a good
case of diarrea, collect some stool from them, make a 10% suspension of the
stuff and preform a negative stain. It will be possible to have a number of
enteric viruses (adeno, rota, HAV, picorna, etc., also bacterial phages) in
the preps. Of course not usually more than one species at a time. Also
there will be various pieces or whole bacteria in it. If the child is a little
older, there will be various undigested bits of food (ex: collegen fibers)
which are neat to look at.
Another side note is that if you find an enteric virus in the stool,
you can tell the parents to quit giving the child antibiotics, unless of
course the pediatrician has had a stool culture preformed and knows that
there is a pathologic bacteria present.
PS Work in a fume hood. Not fun to smell.

Good Luck,
Ed Calomeni
Medical College of Ohio
Toledo, OH
emlab-at-opus.mco.edu




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Tue, 25 Apr 1995 12:34:22 -0500 (CDT)
Subject: EM Film

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From: jfmjfm-at-umich.edu (John F. Mansfield)
Date: Tue, 25 Apr 1995 10:19:59 -0400
Subject: EM Film

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If my memory serves me correctly
4489 and Ilford film are roughly equivalent in speed and grain size,
although the Ilford stuff has the edge (I think).
S0163 is much faster that 4489 because of the large grain but you obviously
pay the price in resolution.
For very fast work I have colleagues that have used DuPont mammography film
(I think 10 to 15 times faster than Ilford).
Does any one have a listing of what the grain size and realtive speed of
the the em films is? Seems to me that this would be a useful list to
maintain.
As far as I know there are the 2 Kodak films, The Ilford (Ciba-Geigy) film,
Agfa EM Film and Fuji EM film. Are there any others?

This info could be part of the Microscopy Web Pages. I will compile the
info if we can collect it.

Jfm.


John Mansfield
North Campus Electron Microbeam Analysis Laboratory
413 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (313)936-3352 FAX (313)936-3352
jfmjfm-at-engin.umich.edu, John.F.Mansfield-at-umich.edu
or jfmjfm-at-umich.edu they all reach me!
URL: http://www.engin.umich.edu/~jfmjfm/jfmjfm.html






From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Tue, 25 Apr 1995 12:24:12 -0500 (CDT)
Subject: Gluing Sapphire/Loctite 460

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From: South Bay Technology :      73531.1344-at-compuserve.com
Date: 25 Apr 95 00:26:18 EDT
Subject: Gluing Sapphire/Loctite 460

Contents Retrieved from Microscopy Listserver Archives
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After reading all of the comings and goings on the listserver concerning the
gluing of sapphire for TEM, I have noticed that we seem to be running parallel
conversations. The initial question that started all of this was from Georges
Veilleux who wanted to bond pieces together in order to make a cross section
sample for TEM. The bond used to make a cross-section sample is intended to be
a permanent bond - therefore, any questions about solvents would become
irrelevent.

The entire conversation got twisted around when Gary Liechty suggested using
Loctite 460 super glue. Super glue is an ideal material to use when mounting a
sample for polishing (such as with a Tripod Polisher), but is not suitable for
bonding together the cross section. Super glues or cyanoacrylates are soluble
in acetone - an ideal characteristic for temporary bonding, but a very
undesirable characteristic for a permanent bond. Materials such as: M-Bond 610
Gatan G1, South Bay Technology Golly G1, and EpoTek 353ND are all permanent
bonding materials and I am not aware of anything that will disolve them after
they have cured.

In short:

M-Bond, SBT Golly G1, Gatan G1, EpoTek 353ND are used for permanent bonding of a
TEM cross section and are stable under the electron beam.

Superglues and other cyanoacrylates such as Loctite 460 are OK for temporary
bonding, are soluble in acetone and are not stable under the electron beam.

If anyone has other questions concerning the bonding of materials, I would be
pleased to offer whatever assistance I can. Please contact me directly and I
will respond as quickly as possible. I hope this information has helped and has
clarified the bonding question!

Best regards-

David Henriks TEL: 800-728-2233
South Bay Technology, Inc. 714-492-2600
1120 Via Callejon FAX: 714-492-1499
San Clemente, CA 92673 e-mail: 75431.1344-at-compuserve.com






From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Tue, 25 Apr 1995 12:35:00 -0500 (CDT)
Subject: holey films

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From: Jane A. Fagerland (708) 935-0104 :      FAGERLAND.JANE-at-igate.abbott.com
Date: Tue, 25 Apr 1995 08:30:00 -0600 (CST)
Subject: holey films

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Mr-Received: by mta RANDB; Relayed; Tue, 25 Apr 1995 09:38:25 -0600
Mr-Received: by mta MCM$RAND; Relayed; Tue, 25 Apr 1995 09:38:29 -0600
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Content-Return: prohibited

I have used a method described by Paul Elsner in the proceedings of the
29th EMSA meeting, page 460. The method produced holey grids that even
my microscope service engineer coveted!

In a chamber or room with 50-70% relative humidity, clean glass slides
are dipped in a Coplin jar of 0.2-0.4% Formvar in ethylene dichloride.
The films are mounted onto grids and then treated with either acetone,
methanol, or chloroform-methanol (1:9) to open up pseudoholes and widen
holes. Varying the concentration of Formvar and relative humidity
results in different hole diameters and numbers of holes. After solvent
treatment, the grids are rinsed in 95% ethanol, 50% ethanol, and
distilled water - about 30 dips in each - and carbon coated.

A few tips to help strip the films from the slides: Rub a clean finger
over the slide a couple of times before you dip it into the Formvar.
After the film is dried, run a razor blade along the edges of the slide
and cut across the top edge of the film. Then breathe on the film until
it's fogged, and dip it into your stripping bath quickly. Sounds hokey,
but it's a method that has never failed me.

Hope this is useful,

Jane A. Fagerland
Dept. Cellular and Microscopic Research
Abbott Laboratories






From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Tue, 25 Apr 1995 12:28:10 -0500 (CDT)
Subject: Re: Alternates to D-19

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From: Daniel Possin :      oemlab-at-u.washington.edu
Date: Mon, 24 Apr 1995 15:24:20 -0700 (PDT)
Subject: Re: Alternates to D-19

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X-Sender: oemlab-at-saul3.u.washington.edu

Hi Frank -

We use a liquid developer from Dupont called DL24 I believe. It is a
graphic arts developer and we dilute it 1:7 for use. You have to buy it
at least 5 gals. at a type. It comes in a polyethelene 'cubitanier'
which collapses as it is emptied. It works well for us.

Dan

On Sun, 23 Apr 1995, Nestor J. Zaluzec-Argonne Nat. Lab. wrote:

} Date: Sun, 23 Apr 1995 7:43:38 -0500 (CDT)
} From: Nestor J. Zaluzec-Argonne Nat. Lab. {ZALUZEC-at-AAEM.AMC.ANL.GOV}
} To: MICROSCOPYLISTZALUZEC-at-AAEM.AMC.ANL.GOV
} Cc: ZALUZEC-at-AAEM.AMC.ANL.GOV
} Subject: Alternates to D-19
}
} From: SMTP%"fskarl-at-goodyear.com" 21-APR-1995 14:21:32.08
} To: MICROSCOPY
} CC:
} Subj: Alternates to D-19
}
} X-Sender: t456b15-at-rds163
} Message-Id: {v01510102abbdbeec0db8-at-[163.243.13.93]}
} Mime-Version: 1.0
} Content-Type: text/plain; charset="us-ascii"
} Date: Fri, 21 Apr 1995 15:01:27 -0500
} To: microscopy-at-aaem.amc.anl.gov
} From: fskarl-at-goodyear.com (Frank Karl)
} Subject: Alternates to D-19
}
} Hi everyone!
}
} We are currently using Kodak D-19 developer and Kodak Electron Microscopy
} Film 4489. We typically develop 123 pieces of film before changing
} developer and due to the relatively large number of potential users (6
} people) we burn through the stock solution of D-19. Mixing D-19 from
} powder is time consuming activity, and in this era of doing more with less
} I would like to find a developer sold as a liquid concentrate. This would
} speed up darkroom maintenance and let us spend more time developing and
} less time preparing to develop.
}
} Can anyone recommend a liquid developer? And while I'm asking, is there a
} better or equivalent film than 4489?
}
}
} Thanks for your input.
}
}
} Frank Karl fskarl-at-goodyear.com
} Goodyear Tire & Rubber Co. Voice 216.796.7818
} Analytical Services - Dept 415B Fax 216.796.3304
} 142 Goodyear Blvd
} Akron, OH 44305
} U.S.A.
}
}
}
}
}
}

%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
% %
% Daniel Possin Work: 206/ 543-7489 %
% Dept. of Ophthalmology RJ-10 FAX: 206/ 543-4414 %
% University of Washington Home: 206/ 778-1714 %
% Seattle WA 98195 USA Email: oemlab-at-u.washington.edu %
% %
% "The chinese expression 'cheung meng ba sui, gong hey fat choy' is %
% equilvalent to Vulcan expression 'live long and prosper'. It's a %
% small universe and getting smaller everyday". %
% %
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Tue, 25 Apr 1995 12:33:06 -0500 (CDT)
Subject: grey levels - film vs digital

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From: sassaroli-at-msvax.mssm.edu
Date: Fri, 03 Dec 1993 09:08:05 -0500
Subject: Re: grey levels - film vs digital

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} } Subject: grey levels - film vs digital
} } }
} } } Krivanek and Mooney (Ultramicroscopy 49:95-108, 1993) state "However,
} } } whereas each pixel of the SSC (slow scan CCD) is capable of faithfully
} } } capturing an intensity between 0 and 2(to the 14th power),
} }
} } = 8 x 10E3 THAT is a HOT CCD !
} }
} } I seem to recollect this as a kind of wondrous
} } upper limit, How many charges
} } fit in a charge bucket vs the noise floor?
}
} The full-well capacity of a CCD can be as high as half a million e/pixel
} (as in TEK1024 CCDs, with a pixel size of 24 microns), and the noise level
} is only ~10 e/sec when cooled to -40C, so a dynamic range of 14 bits can be
} readily achieved. But, the true dynamic range is shot-noise limited. Say
} your image is formed with 1,000,000 primary electrons/pixel (which is very,
} very high), the dynamic range is 1000.
}
} ...
} } } each pixel of film can capture only a few gray levels
} } } (roughly equivalent to the number of silver grains
}
} I agree.
}
} } } that can fit into the area of one pixel without
} } } overlapping)". Buonaquisti (Microscopy Today 95(1):12-13, 1995) states
} } } film "is an anlog process and, as such, one can argue that sheet film has
} } } an infinite gray scale resolution". Can anyone comment on this?
}
} Infinite? I read that too. Keep in mind that artcile was not refereed.
}
} Gary Fan
} UC San Diego


I agree with the first half of Gary Fang's answer. Indeed, the dynamic
range of cooled slow-scan CCD's can attain values in excess of } = 40,000
(we have an EG&G camera with a Thompson chip which does that when cooled to
-65C).
The second half of the answer, I believe, confuses dynamic range with
signal/noise and whether the image is dark noise limited or shot noise
limited: this will depend on the level of the signal (in
photoelectrons/pixel/sec) as compared to the dark noise (in
electrons/pixel/sec) due to the thermally excited electrons generated in
the silicon and to the added electronic noise in the digitization
electronics and in the bias voltage applied to the A/D converter.
The shot noise is a phenomenon of quantum mechanical origin and cannot be
eliminated, only minimized by accumulating as much signal as possible! In
the example given, the image would be of very high quality indeed
(S/N=1000/1)!!!
Signal levels in low-light level fluorescence microscopy, which is the
application I am most familiar with, almost never exceed 10,000
photoelectrons/pixel/sec with a pixel size of 20x20 micron.

No film can come even close to the dynamic range of a modern CCD: as
explained by Nestor, film can be used to capture signals of various
intensities by adjusting the f-stops on the camera, but the intrascene
dynamic range (the number of different intensity levels that can be
captured in the same image) is very limited (certainly less than 100).

Best regards,
Massimo Sassaroli

_________________________________
Massimo Sassaroli, D.Sc.
Dept. of Physiology & Biophysics
Box 1218
Mount Sinai School of Medicine
1 Gustave L. Levy Pl.
New York, NY 10029-6574

sassaroli-at-msvax.mssm.edu







From: Michael O'Keefe :      Michael_O'Keefe-at-macmail7.lbl.gov
Date: 25 Apr 1995 13:56:45 U
Subject: EM Technician Position

Contents Retrieved from Microscopy Listserver Archives
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Message-Id: {n1413304036.39952-at-macmail7.lbl.gov}




From: Michael O'Keefe :      Michael_O'Keefe-at-macmail7.lbl.gov
Date: 25 Apr 1995 13:56:45 U
Subject: EM Technician Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The National Center for Electron Microscopy has a vacancy for an electron
microscope technician. For details see --

http://www.lbl.gov/LBL-Documents/Current-Jobs-Offerings/Apr-21-95.Technical.html


HOW TO APPLY for this position (Job MSD/3280) --

To apply for job MSD/3280, submit a resume to Lawrence Berkeley Laboratory.
Following our receipt of your resume, you will receive a letter of
acknowledgment. If you do not have a resume, please complete an LBL
application form: this will not affect your being considered for employment.

Resumes will be retained in our active employment file for six months. During
this period, you may refer to a specific job number by including it in your
one-page cover letter or by writing it on the back of your resume. Individuals
interviewed will be notified of selection or nonselection for the position.

Submit your resume by mail to --
The Staffing Office, 1 Cyclotron Road, MS 938A, Berkeley, CA 94720,
or, if you prefer, via e-mail to the LBL Recruitment Coordinator,
RLBoucher-at-LBL.gov.
Please do not send your resume directly to, or otherwise contact, the NCEM.


DESCRIPTION
(Job MSD/3280) --

Research Technician, Senior

1.Job MSD/3280
2.Materials Sciences Division
3.NCEM
4.$12.10-$20.53/Hr.

DUTIES:
Essential --
Responsible for daily operation and maintenance of several transmission
electron microscopes. Duties include daily start-up, alignment, trouble
shooting, user training in basic operation, alignment, microanalysis, and
high-resolution imaging. Effective interaction with users and close
coordination with Center staff and with manufacturers' service representatives
are essential. Flexibility to work hours assigned based on Center needs.

QUALIFICATIONS:
Essential --
Demonstrated ability in electron microscopy with emphasis on physical sciences.
Detailed knowledge of operating principles of transmission electron
microscopes, with demonstrated ability to maintain, operate and troubleshoot
advanced and support transmission electron microscopes. Demonstrated
experience in microscope alignment procedures for phase contrast imaging.
Working knowledge of diffractogram analysis for diagnosis of defocus,
astigmatism, beam tilt, sample tilt, drift, damping, information transfer etc.
Sufficient understanding of principles of electron beam imaging, diffraction
and crystallography to permit operating of the JEOL ARM-1000 under the
scientific guidance of a user. Working knowledge of vacuum systems, specimen
holders, high voltage systems and control electronics found in modern electron
microscopes. Ability to isolate malfunctions in an electron microscope and to
rectify failures. Practical experience with thin foil preparation methods.
Elementary knowledge of principles of materials science. Elementary knowledge
of computers for digital acquisition of images and microanalytical spectra.
Ability to learn new techniques of imaging, diffraction, microanalysis and
computer image processing. Ability to work with a minimum of supervision. Good
communication skills for clear and efficient interaction with users and NCEM
scientific staff scientists.
Marginal --
B.S. or B.A. in general sciences, physics, or electronics, and coursework in
electron microscopy.

CLOSING DATE: Open until filled.






From: Michael O'Keefe :      Michael_O'Keefe-at-macmail7.lbl.gov
Date: 25 Apr 1995 13:49:10 U
Subject: Position available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Message-Id: {n1413304642.4716-at-macmail7.lbl.gov}
"Microscopy" {Microscopy-at-aaem.amc.anl.gov}
Cc: "Nestor ZALUZEC" {ZALUZEC-at-aaem.amc.anl.gov}
X-Mailer: Mail*Link SMTP/QM 3.0.0

Subject: Time:11:40 AM
OFFICE MEMO Position available Date:4/24/95

The National Center for Electron Microscopy has a vacancy for an electron
microscope technician. For details see --

http://www.lbl.gov/LBL-Documents/Current-Jobs-Offerings/Apr-21-95.Technical.html


HOW TO APPLY for this position (Job MSD/3280) --

To apply for job MSD/3280, submit a resume to Lawrence Berkeley Laboratory.
Following our receipt of your resume, you will receive a letter of
acknowledgment. If you do not have a resume, please complete an LBL
application form: this will not affect your being considered for employment.

Resumes will be retained in our active employment file for six months. During
this period, you may refer to a specific job number by including it in your
one-page cover letter or by writing it on the back of your resume. Individuals
interviewed will be notified of selection or nonselection for the position.

Submit your resume by mail to --
The Staffing Office, 1 Cyclotron Road, MS 938A, Berkeley, CA 94720,
or, if you prefer, via e-mail to the LBL Recruitment Coordinator,
RLBoucher-at-LBL.gov.
Please do not send your resume directly to, or otherwise contact, the NCEM.


DESCRIPTION
(Job MSD/3280) --

Research Technician, Senior

1.Job MSD/3280
2.Materials Sciences Division
3.NCEM
4.$12.10-$20.53/Hr.

DUTIES:
Essential --
Responsible for daily operation and maintenance of several transmission
electron microscopes. Duties include daily start-up, alignment, trouble
shooting, user training in basic operation, alignment, microanalysis, and
high-resolution imaging. Effective interaction with users and close
coordination with Center staff and with manufacturers' service representatives
are essential. Flexibility to work hours assigned based on Center needs.

QUALIFICATIONS:
Essential --
Demonstrated ability in electron microscopy with emphasis on physical sciences.
Detailed knowledge of operating principles of transmission electron
microscopes, with demonstrated ability to maintain, operate and troubleshoot
advanced and support transmission electron microscopes. Demonstrated
experience in microscope alignment procedures for phase contrast imaging.
Working knowledge of diffractogram analysis for diagnosis of defocus,
astigmatism, beam tilt, sample tilt, drift, damping, information transfer etc.
Sufficient understanding of principles of electron beam imaging, diffraction
and crystallography to permit operating of the JEOL ARM-1000 under the
scientific guidance of a user. Working knowledge of vacuum systems, specimen
holders, high voltage systems and control electronics found in modern electron
microscopes. Ability to isolate malfunctions in an electron microscope and to
rectify failures. Practical experience with thin foil preparation methods.
Elementary knowledge of principles of materials science. Elementary knowledge
of computers for digital acquisition of images and microanalytical spectra.
Ability to learn new techniques of imaging, diffraction, microanalysis and
computer image processing. Ability to work with a minimum of supervision. Good
communication skills for clear and efficient interaction with users and NCEM
scientific staff scientists.
Marginal --
B.S. or B.A. in general sciences, physics, or electronics, and coursework in
electron microscopy.

CLOSING DATE: Open until filled.






From: Michael O'Keefe :      Michael_O'Keefe-at-macmail7.lbl.gov
Date: 25 Apr 1995 09:19:40 U
Subject: Position available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Message-Id: {n1413320776.33851-at-macmail7.lbl.gov}
"Microscopy" {Microscopy-at-aaem.amc.anl.gov}
Cc: "Nestor ZALUZEC" {ZALUZEC-at-aaem.amc.anl.gov}
X-Mailer: Mail*Link SMTP/QM 3.0.0

Subject: Time:11:40 AM
OFFICE MEMO Position available Date:4/24/95

The National Center for Electron Microscopy has a vacancy for an electron
microscope technician. For details see --

http://www.lbl.gov/LBL-Documents/Current-Jobs-Offerings/Apr-21-95.Technical.html


HOW TO APPLY for this position (Job MSD/3280) --

To apply for job MSD/3280, submit a resume to Lawrence Berkeley Laboratory.
Following our receipt of your resume, you will receive a letter of
acknowledgment. If you do not have a resume, please complete an LBL
application form: this will not affect your being considered for employment.

Resumes will be retained in our active employment file for six months. During
this period, you may refer to a specific job number by including it in your
one-page cover letter or by writing it on the back of your resume. Individuals
interviewed will be notified of selection or nonselection for the position.

Submit your resume by mail to --
The Staffing Office, 1 Cyclotron Road, MS 938A, Berkeley, CA 94720,
or, if you prefer, via e-mail to the LBL Recruitment Coordinator,
RLBoucher-at-LBL.gov.
Please do not send your resume directly to, or otherwise contact, the NCEM.


DESCRIPTION
(Job MSD/3280) --

Research Technician, Senior

1.Job MSD/3280
2.Materials Sciences Division
3.NCEM
4.$12.10-$20.53/Hr.

DUTIES:
Essential --
Responsible for daily operation and maintenance of several transmission
electron microscopes. Duties include daily start-up, alignment, trouble
shooting, user training in basic operation, alignment, microanalysis, and
high-resolution imaging. Effective interaction with users and close
coordination with Center staff and with manufacturers' service representatives
are essential. Flexibility to work hours assigned based on Center needs.

QUALIFICATIONS:
Essential --
Demonstrated ability in electron microscopy with emphasis on physical sciences.
Detailed knowledge of operating principles of transmission electron
microscopes, with demonstrated ability to maintain, operate and troubleshoot
advanced and support transmission electron microscopes. Demonstrated
experience in microscope alignment procedures for phase contrast imaging.
Working knowledge of diffractogram analysis for diagnosis of defocus,
astigmatism, beam tilt, sample tilt, drift, damping, information transfer etc.
Sufficient understanding of principles of electron beam imaging, diffraction
and crystallography to permit operating of the JEOL ARM-1000 under the
scientific guidance of a user. Working knowledge of vacuum systems, specimen
holders, high voltage systems and control electronics found in modern electron
microscopes. Ability to isolate malfunctions in an electron microscope and to
rectify failures. Practical experience with thin foil preparation methods.
Elementary knowledge of principles of materials science. Elementary knowledge
of computers for digital acquisition of images and microanalytical spectra.
Ability to learn new techniques of imaging, diffraction, microanalysis and
computer image processing. Ability to work with a minimum of supervision. Good
communication skills for clear and efficient interaction with users and NCEM
scientific staff scientists.
Marginal --
B.S. or B.A. in general sciences, physics, or electronics, and coursework in
electron microscopy.

CLOSING DATE: Open until filled.






From: Michael O'Keefe :      Michael_O'Keefe-at-macmail7.lbl.gov
Date: 24 Apr 1995 13:51:55 U
Subject: EM Tech position available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Message-Id: {n1413390807.23289-at-macmail7.lbl.gov}
"Microscopy" {Microscopy-at-aaem.amc.anl.gov}
Cc: "Nestor ZALUZEC" {ZALUZEC-at-aaem.amc.anl.gov}
X-Mailer: Mail*Link SMTP/QM 3.0.0

Reply to: EM Tech position available

The National Center for Electron Microscopy has a vacancy for an electron
microscope technician. For details see --

http://www.lbl.gov/LBL-Documents/Current-Jobs-Offerings/Apr-21-95.Technical.html


HOW TO APPLY for this position (Job MSD/3280) --

To apply for job MSD/3280, submit a resume to Lawrence Berkeley Laboratory.
Following our receipt of your resume, you will receive a letter of
acknowledgment. If you do not have a resume, please complete an LBL
application form: this will not affect your being considered for employment.

Resumes will be retained in our active employment file for six months. During
this period, you may refer to a specific job number by including it in your
one-page cover letter or by writing it on the back of your resume. Individuals
interviewed will be notified of selection or nonselection for the position.

Submit your resume by mail to --
The Staffing Office, 1 Cyclotron Road, MS 938A, Berkeley, CA 94720,
or, if you prefer, via e-mail to the LBL Recruitment Coordinator,
RLBoucher-at-LBL.gov.
Please do not send your resume directly to, or otherwise contact, the NCEM.


DESCRIPTION
(Job MSD/3280) --

Research Technician, Senior

1.Job MSD/3280
2.Materials Sciences Division
3.National Center for Electron Microscopy
4.$12.10-$20.53/Hr.

DUTIES: Essential --
Responsible for daily operation and maintenance of several transmission
electron microscopes. Duties include daily start-up, alignment, trouble
shooting, user training in basic operation, alignment, microanalysis, and
high-resolution imaging. Effective interaction with users and close
coordination with Center staff and with manufacturers' service representatives
are essential. Flexibility to work hours assigned based on Center needs.
QUALIFICATIONS: Essential --
Demonstrated ability in electron microscopy with emphasis on physical sciences.
Detailed knowledge of operating principles of transmission electron
microscopes, with demonstrated ability to maintain, operate and troubleshoot
advanced and support transmission electron microscopes. Demonstrated
experience in microscope alignment procedures for phase contrast imaging.
Working knowledge of diffractogram analysis for diagnosis of defocus,
astigmatism, beam tilt, sample tilt, drift, damping, information transfer etc.
Sufficient understanding of principles of electron beam imaging, diffraction
and crystallography to permit operating of the JEOL ARM-1000 under the
scientific guidance of a user. Working knowledge of vacuum systems, specimen
holders, high voltage systems and control electronics found in modern electron
microscopes. Ability to isolate malfunctions in an electron microscope and to
rectify failures. Practical experience with thin foil preparation methods.
Elementary knowledge of principles of materials science. Elementary knowledge
of computers for digital acquisition of images and microanalytical spectra.
Ability to learn new techniques of imaging, diffraction, microanalysis and
computer image processing. Ability to work with a minimum of supervision. Good
communication skills for clear and efficient interaction with users and NCEM
scientific staff scientists.
Marginal --
B.S. or B.A. in general sciences, physics, or electronics, and coursework in
electron microscopy.
CLOSING DATE: Open until filled.






From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Thu, 27 Apr 1995 8:15:50 -0500 (CDT)
Subject: Never, Never Do This!!!

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Subj: NEVER, DO THIS!!!

All Subscribers....

Please, Pleeease, Pleeeeeeeeeease

Never, never submit a posting to the temporary address:

MicroscopyListZaluzec-at-aaem.amc.anl.gov

always submit your messages to the correct address:

Microscopy-at-aaem.amc.anl.gov.

The temporary address "MicroscopyListZaluzec-at-aaem.amc.anl.gov"
was set up so that I can monitor and remove any problems
which were created by the series of bouncing messages, DNS/Router
crashes and several other things that went wrong last week.
I have been manually checking each message which comes through
and trashing the various error messages etc... and only
forwarding the "real" mail. This minimizes your Email traffic
and slows things down, and complicates my life, since I now
check each message before it goes out. (BTW that is one reason
for the longer delays now between the time you send a message and
you see it back as a posting to the list).

If you post to the temporary address you WILL likely start
another loop (Yes a person who will remain nameless did this today).
I think I caught it before it got too far, but I'm sure several iterations
went out. I have shut down the server and trashed any messages in the queue.
This means that some things will likely be lost.

Please don't try to second guess the system.
Just use the old address and thing will be fine, albeit abit
slow for the next few more days....



Your Frustrated and Temporarily Unfriendly Neighborhood SysOp.

Nestor





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Thu, 27 Apr 1995 8:51:05 -0500 (CDT)
Subject: d-19 storage

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From: Mrs Priscilla Maartens :      MAARTENP-at-biology.und.ac.za
Date: Wed, 26 Apr 1995 08:09:58 +0200 (SAST)
Subject: d-19 srorage

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Message-ID: {MAILQUEUE-101.950426080958.523-at-biology.und.ac.za}

Bob Josephs suggests making up your D-19 stco and then storing it in
brown bottles.
What about making up your stock and freezing aliquots? You then
freeze the quantity most suited to the final dilution you will use.




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Thu, 27 Apr 1995 8:58:00 -0500 (CDT)
Subject: Wanted - Stereomicroscope

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From: Ann Webb :      Ann_Webb-at-uow.edu.au
Date: 27 Apr 1995 11:56:53 +1000
Subject: Wanted - Stereomicroscope

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Stereomicroscope wanted - zoom preferred. Please send details and asking
price to A.Webb-at-uow.edu.au.

Ann Webb




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Thu, 27 Apr 1995 8:54:35 -0500 (CDT)
Subject: recycle silver paste?

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From: Tina Carvalho :      tina-at-ahi.pbrc.Hawaii.Edu
Date: Wed, 26 Apr 1995 10:26:53 -1000 (HST)
Subject: recycle silver paste?

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Aloha, all-

I have some 150 grams of dried-up colloidal silver paste that is not worth
trying to get back into solution, partly because it costs more to ship
the solvent to Hawaii than to purchase new paste. Is it worth trying to
recycle somehow? What all is in it besides silver? Is it pretty pure?
I'm not even sure there's anyone in Hawaii who recycles silver from darkroom
chemicals.

Besides the osmium, what else should us environmentally-concerned
microscopists be trying to conserve/recycle?

A belated Happy Earth Day to you all!

Mahalo,
Tina Weatherby Carvalho
Biological EM Facility
University of Hawaii





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Thu, 27 Apr 1995 9:07:25 -0500 (CDT)
Subject: Gatan Imaging Filter

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From: loewe-at-uni-bonn.de (Andreas Loewe)
Date: Thu, 27 Apr 1995 08:46:49 +0100
Subject: GIF - Gatan Imaging Filter

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X-Sender: UNC900-at-131.220.221.1
Message-Id: {v01510100abc4fa41bef0-at-[131.220.128.16]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Hi all -

I would like to get and exchange information about the Gatan Imaging
Filter. We will receive ours at the end of the month, so we are absolute
beginners. What I like to see are hints about the little things you just
don't know because you have not enough experience.

Every help, sugestions and ideas is highly welcomed.


Andreas
UofB






From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Thu, 27 Apr 1995 8:49:06 -0500 (CDT)
Subject: Re: CPD of millipore filters

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From: Alan S. Pooley :      pooley-at-ahab.rutgers.edu
Date: Wed, 26 Apr 1995 09:16:58 -0400 (EDT)
Subject: Re: CPD of millipore filters

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Re filters for cpd, I have made and used for years an aluminium holder
about 15 mm on a side, ~cubic, consisting of folded thin alum sheet
which switches back and forth in a multiple 'S' fashion so that individual
slots about 1.5 mm wide by 15 * 15 mm are made. I have about 8 or 10
slots per holder. The multiple s is held together with a wide 'U' shape
that holds the side and bottom of the holder. Individual filters are
slipped down into the slots (I do this in a 20 or 30 mm beaker filled with
enough alcohol that the filter stays emersed as it is fed into the slot,
the filter is quickly transfered from the previous container to avoid
drying by evaporation) By the way, I much prefer nucleopore filters
to millipore, better background for SEM. I do not believe that material
falls off the filter unless too much matrerial is applied. A good rule
is to put about 500,000 cells on a 12 mm dia filter. Call me if you
can't figure out the design of the holder. It took me 10 minutes to make
with simple tools.

Alan S. Pooley ,PhD Bivalve shell SEM & shape analysis
SEM/Morphometrics lab
Marine & Coastal Sciences
Rutgers University
908 932 8959 ext 225
Pooley-at-ahab.rutgers.edu





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Thu, 27 Apr 1995 8:49:23 -0500 (CDT)
Subject: Histo-resins

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From: JOHN PUTTERILL :      JOHN-at-moon.ovi.ac.za
Date: Wed, 26 Apr 1995 08:45:47 CAT
Subject: Histo-resins

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Hello Microscopists

Would anyone out there have any experience with a resin called
"Immuno-Bed" Embedding Resin (Polysciences?). It is in the EM Sciences
catalogue and is claimed to be "A low viscosity media for light
microscopy and immuno histochemistry techniques". I would like to
know whether it would be suitable for staining of animal tissue using
routine histo staining techniques, specifically H&E, PAS, Masson's
Trichrome, Verhoeff's Elastic Stain and Periodic Acid Silver
Haematoxylin Methamine. Comment regarding its staining
reproducibility, ease of cutting etc are also welcome. Any
suggestions of alternatives to the "Immuno-Bed" would also be
appreciated.

Please correspond with me directly. Thanks in advance.

John////////
////////////





-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
-at- John F. Putterill -at-
-at- Electron Microscopy Unit Tel: (Int) 27-12-529-9174 -at-
-at- Pathology Section Fax: (Int) 27-12-529-9165 -at-
-at- Onderstepoort Veterinary Institute E-mail: john-at-moon.ovi.ac.za -at-
-at- Private Bag X05 -at-
-at- Onderstepoort 0110 -at-
-at- South Africa -at-
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Thu, 27 Apr 1995 14:33:42 -0500 (CDT)
Subject: Validation of EDS Equipment

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From: BARBARA.HARTMAN-at-1773.220.SCHERING-PLOUGH.sprint.com
Date: Thu, 27 Apr 1995 10:44:20 -0400
Subject: VALIDATION

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X400-Received: by mta merit in /PRMD=internet/ADMD=telemail/C=us/; Relayed; Thu, 27 Apr 1995 10:56:16 -0400
X400-Received: by /ADMD=TELEMAIL/C=US/; Relayed; Thu, 27 Apr 1995 10:46:14 -0400
X400-Received: by /PRMD=SCHERING-PLOUGH/ADMD=TELEMAIL/C=US/; Relayed; Thu, 27 Apr 1995 10:44:20 -0400

Greetings,

I have to validate an EDAX DX-4. I am in the pharmaceutical
industry and validation for the FDA on our equipment is top priority.
Most of the advice that I have been receiving is more calibration than
validation. What do others do to validate such a piece of equipment?


Thank you!!


Barbara Hartman
Schering-Plough Research
Lafayette, NJ 07848
(201) 579-4343
(201) 579-4211 FAX
E-Mail: Barbara.Hartman-at-1773.220.Schering-Plough.sprint.com






From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Thu, 27 Apr 1995 8:51:55 -0500 (CDT)
Subject: TEM films and development

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From: cook-at-aaem.amc.anl.gov (Russell E. Cook)
Date: Wed, 26 Apr 1995 12:26:34 -0600
Subject: TEM films and development

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In answer to John Mansfield's questions, there was another EM film in 1990,
but I haven't looked lately to see whether it is still available:
Mitsubishi MEM.

Let me refer you to a paper by Y. Kamiya and T. Arii, J. Electron Microscoy
39: 351-355 (1990). It shows that Kodak 4489 and Fuji FG are very similar.
Mean sizes of developed grains in the Fuji film were measured at 0.24 and
0.33 micron for development times of 4 and 10 minutes, respectively. The
Mitsubishi film was shown to be 5 times as sensitive as the Kodak or Fuji
films at a development time of 4 minutes. Each film used a different
developer.

Several years ago, we learned of a developing technique which uses a 2-part
developer (Daniel Morse at Los Alamos National Laboratory). He (and we)
used a particular product called Diafine, but there are other 2-part
developers available. This procedure holds back development in areas of
high exposure and increases development in areas of low exposure. The
result is a negative which can be printed easily compared to a negative
developed in a single-solution developer. Diafine also increases the
effective film speed.

I have some data sheets from Kodak which compare 4489 and SO-163. I do not
have rms granularity numbers for the films, but Kodak did provide relative
electron speeds (sensitivities) for electron energies between 50 and 100
keV. These sensitivities were measured at a density of 1.0 above gross fog
and are the reciprocal of the exposure measured in electrons per square
micron.

Film Electron Speed Developer Devel. Time

SO-163 2.2 D19, full strength 12 min.

SO-163 0.8 1 part D19 to 2 parts 4 min.
of water

SO-163 0.15 (1985) D19, full strength, 2 min.
or 0.4 (1990) plus 1 gm/l of Kodak
Anti-Fog #1

4489 0.4 1 part D19 to 2 parts 4 min.
of water

By the way, the Kodak technical sheet for SO-163 recommends maintaining the
relative humidity above 25% to mitigate against static build-up (and
discharges) on the film.


Russell E. Cook
Electron Microscopy Center for Materials Research
Argonne National Laboratory






From: vickie-at-macc.wisc.edu
Date: Thu, 27 Apr 1995 8:54:17 -0500 (CDT)
Subject: 2 Photon Meeting Announcement

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Message-Id: {25042514005049-at-vms2.macc.wisc.edu}

Meeting Announcement

Symposium on

"INTEGRATED MICROSCOPY"

September 29 to October 1, 1995

at

The Wisconsin Center
702 Langdon Street
Madison, WI 53706

organized by the

Integrated Microscopy Resource
University of Wisconsin-Madison

********************************************************************************
Presentations in this symposium will focus on biological problems for which
a combination of microscopies (i.e. integrated microscopy) has been used.
In this way the speakers will demonstrate by example the power, potential
and limitations of various microscopical techniques. The techniques which
will be discussed include: DIC, confocal, 2-photon excitation imaging,
SEM, TEM, cryo-specimen preparation, and AFM.
********************************************************************************

FRIDAY (Evening), September 29, 1995
6:00 - 7:55 RECEPTION
8:00 - 8:45 Ken Jacobson, Univ. of N. Carolina-Chapel Hill
8:50 - 9:35 John Sedat, Univ. of California-San Francisco
9:40 - 10:25 Edward Salmon, Univ. of N.Carolina-Chapel Hill
SATURDAY, September 30, 1995
8:30 - 9:15 D. Lansing Taylor, Carnegie-Mellon University
9:20 - 10:05 Jeff Lichtman, Washington University
10:10 - 10:40 COFFEE BREAK
10:45 - 11:30 John White, University of Wisconsin-Madison
11:35 - 12:20 Steven Smith, Stanford University
12:25 - 1:25 LUNCH (on your own)
1:30 - 2:15 Gary Borisy, University of Wisconsin-Madison
2:20 - 3:05 Ralph Albrecht, Univ. of Wisconsin-Madison
3:10 - 3:55 Paul Bridgman, Washington University-Madison
5:00 - 6:00 EXHIBITOR'S SOCIAL
6:00 - 8:00 BUFFET DINNER
SUNDAY (Morning), October 1, 1995
9:00 - 9:45 Kent McDonald, Univ. of California-Berkley
9:50 - 10:35 Stanley Erlandsen, University of Minnesota
10:40 - 11:10 COFFEE BREAK
11:15 - 12:00 Hans Ris, University of Wisconsin-Madison
12:05 - 12:50 Eric Henderson, Iowa State University


Fees:
General Registration $100.00 (Late fee: $130.00)
(Includes: Fri. Reception, Sat. Social & Dinner, Coffee
Breaks and Materials)
Local Registration $ 80.00 (Late fee: $110.00)
(Includes: Fri. Reception, Sat. Social, Coffee Breaks and
Materials)
Guest Ticket - Saturday Dinner $ 20.00 (On-site Unavailable)


Interested in attending?

Consult our Web site for additional information:

http://www.bocklabs.wisc.edu/imr/imr.html


To receive a brochure and registration form write to:
IMR
University of Wisconsin-Madison
1675 Observatory Drive
Madison, WI 53706

or email: imradmin-at-calshp.cals.wisc.edu



Following the symposium, there will be a two-day workshop at the IMR. We
will be presenting lectures and provide "hands-on" experience for the
following techniques:
* 2-photon excitation imaging
* 4D DIC imaging
* cryo-SEM
* high pressure freezing
* reversible embedment for SEM and TEM

Workshop attendence will be limited to 25 students. A letter of
application is required. Fee: $150.00.





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Thu, 27 Apr 1995 22:21:30 -0500 (CDT)
Subject: Image archive system

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From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Thu, 27 Apr 1995 22:23:12 -0500 (CDT)
Subject: Polaroid Micrograph Contest???

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Message-Id: {9504271535.AA03439-at-riker.ml.wpafb.af.mil}




From: Tina Carvalho :      tina-at-ahi.pbrc.Hawaii.Edu
Date: Thu, 27 Apr 1995 10:37:40 -1000 (HST)
Subject: Polaroid Micrograph Contest???

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Does anyone know if there will be a Polaroid International Micrograph
Contest this year? I've not been able to get hold of anyone at Polaroid
who knows...

Aloha,
Tina Carvalho
Biological EM Facility
University of Hawaii





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Thu, 27 Apr 1995 22:31:10 -0500 (CDT)
Subject: Term lbs.

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From: Campbell36-at-aol.com
Date: Thu, 27 Apr 1995 22:02:46 -0400
Subject: Term lbs.

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Can anyone tell me the origin of the term (lbs.) as it relates to the unit of
measure pounds?

Thanks

Jim Campbell




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Thu, 27 Apr 1995 22:25:58 -0500 (CDT)
Subject: Re: grey levels - film vs digital

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From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Thu, 27 Apr 1995 22:25:03 -0500 (CDT)
Subject: RE-SourceVacBk

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From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 27 Apr 1995 16:12:19 -0400
Subject: RE-SourceVacBk

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Message-ID: {n1413123023.39654-at-mse.engin.umich.edu}

Subject: Time: 4:08 PM
OFFICE MEMO RE:SourceVacBk Date: 4/27/95

Peggy:
Thanks for inquiring about my vacuum book. The following is the
information you requested:
Title: Vacuum Methods in Electron Microscopy
Author: Wilbur C. Bigelow
Series: Practical Methods in Electron Microscopy, Vol. 15,
Audrey M. Glauert, Editor
Soft cover: ISBN 1-85578-053-4, 492 pp/116 illus/1994/$80
Hard cover: ISBN 1-85578-052-6, 492 pp/116 illus/1994/$175
In US and Canada, order from: Portland Press, Ashgate Publishing Co.,
Old Post Road, Brookfield, VT, 05036-9704. U.S.A.
Ph: 802-276-3162; Fx: 802-276-3837
In Europe, order from: Portland Press Ltd., Commerce Way, Colchester
CO2 8HP, UK Ph: 0206-796351; Fx: 0206-799331
I apologize for the fact that the prices are a bit higher than one might
wish them to be; however, the book is nearly 500 pages long, and was a high
quality production by Cambridge Univ. Press. It is printed on high quality
paper, and the figures are of very high quality. In addition, I think you
will find it to be filled with practically useful operational information.
The two reviews I have seen so far have been pleasingly favourable. A quote
from one for the Italian EM Society: "It is a (sic) almost perfect book,
practical and convincing. The Series title is masterly (sic) fulfilled by
practical hints, numerical data, references to everyday life, operation
procedures, and suggestions, which, if followed, will save trouble and time
for microscopists and will optimise their results". If you buy it, I hope
you like it and find it useful.
Wil Bigelow (bigelow-at-umich.edu)





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Thu, 27 Apr 1995 22:26:41 -0500 (CDT)
Subject: d-19 storage

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From: Daniel Possin :      oemlab-at-u.washington.edu
Date: Thu, 27 Apr 1995 11:20:42 -0700 (PDT)
Subject: Re: d-19 storage

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X-Sender: oemlab-at-saul1.u.washington.edu


} Subject: d-19 storage
}
} From: SMTP%"MAARTENP-at-biology.und.ac.za" 26-APR-1995 09:27:38.06
} To: ZALUZEC
} CC:
} Subj: d-19 srorage
}
} Message-ID: {MAILQUEUE-101.950426080958.523-at-biology.und.ac.za}
} From: Mrs Priscilla Maartens {MAARTENP-at-biology.und.ac.za}
} To: zaluzec-at-aaem.amc.anl.gov
} Date: Wed, 26 Apr 1995 08:09:58 +0200 (SAST)
} Subject: d-19 srorage
} X-pmrqc: 1
} Priority: normal
} X-mailer: PMail v3.0 (R1a)
}
} Bob Josephs suggests making up your D-19 stco and then storing it in
} brown bottles.
} What about making up your stock and freezing aliquots? You then
} freeze the quantity most suited to the final dilution you will use.
}
I don't think it will work. The salts in the developer (sodium sulfite,
etc.) will come out of solution and need to be redissolved before use,
which maybe difficult (or impossible) depending on the other developer
constituents.

Sorry-

Dan

%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
% %
% Daniel Possin Work: 206/ 543-7489 %
% Dept. of Ophthalmology RJ-10 FAX: 206/ 543-4414 %
% University of Washington Home: 206/ 778-1714 %
% Seattle WA 98195 USA Email: oemlab-at-u.washington.edu %
% %
% "The chinese expression 'cheung meng ba sui, gong hey fat choy' is %
% equilvalent to Vulcan expression 'live long and prosper'. It's a %
% small universe and getting smaller everyday". %
% %
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Fri, 28 Apr 1995 8:54:18 -0500 (CDT)
Subject: Re: Term lbs.

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From: DOUG ARRELL :      ARRELL-at-fs-iam-1
Date: Fri, 28 Apr 1995 08:16:39 GMT+0200
Subject: Re: Term lbs.

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Message-Id: {MAILQUEUE-101.950428081639.544-at-FS-IAM-1.JRC.NL}

} Date: Thu, 27 Apr 1995 22:31:10 -0500 (CDT)
} From: "Nestor J. Zaluzec-Argonne Nat. Lab." {ZALUZEC-at-aaem.amc.anl.gov}
} To: MICROSCOPYLISTNESTOR-at-aaem.amc.anl.gov
} Cc: ZALUZEC-at-aaem.amc.anl.gov
} Subject: Term lbs.

} } From: SMTP%"Campbell36-at-aol.com" 27-APR-1995 20:06:46.60

} Can anyone tell me the origin of the term (lbs.) as it relates to the unit of
} measure pounds?
}
} Thanks
}
} Jim Campbell

It comes from the latin word libra which was the original term and is
still used in French, Spanish and other latin languages. The word
'pound' is the germanic equivalent also used in Dutch.

Doug


******************************************
Dr Douglas Arrell
Mechanical Properties and Joining Division
Institute for Advanced Materials
Joint Research Centre
European Commission
Westerduinweg 2
1755 ZG Petten
The Netherlands

Tel. (+31) 2246 5287
Fax. (+31) 2246 1917
E-Mail Arrell-at-JRC.NL
*********************************




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Fri, 28 Apr 1995 9:00:34 -0500 (CDT)
Subject: Wanted - Stereomicroscope

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From: ALAN . STONE :      73004.1733-at-compuserve.com
Date: 28 Apr 95 06:57:52 EDT
Subject: Wanted - Stereomicroscope

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we have a B&L stereozoom 7 in great shape. magnification varies from 10X
to 70X and comes with a supplemental .5X lens to extend the magnification
range from 5X to 35X. The asking price is US $1500.

Please reply with your level of interest.

Alan Stone
ASTON
312/528-9830




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Fri, 28 Apr 1995 9:01:19 -0500 (CDT)
Subject: JWS 7500E SEM

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From: S_MCGARVEY-at-SAL9K.dnet-at-gmd.fujitsu.com
Date: Fri, 28 Apr 1995 04:38:49 +0800
Subject: JWS 7500E SEM

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Greetings,

I am interested in hearing from anyone who has worked with a JWS
7500E SEM for defect analysis on silicon wafers. Any information
anyone can offer would be a great help!


Many thanks!



Steve McGarvey
Photolithograhy Process Tech
Fujitsu Microelectronics, Inc.
Gresham, Oregon 97030

e-mail: s_mcgarvey-at-sal9k.dnet-at-gmd.fujitsu.com
Fax: (503) 669-6131
Phone: (503) 669-6118

"There are lies, damned lies, and statistics."





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Fri, 28 Apr 1995 9:02:23 -0500 (CDT)
Subject: Coverslip removal

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From: Fermin, Cesar :      fermin-at-tmc.tulane.edu
Date: 28 Apr 1995 06:48:25 -0600
Subject: FW: Coverslip removal

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Message-ID: {n1413070635.56712-at-postoffice.tmc.tulane.edu}



I will appreciate any trick (besides immersion in xylene) for removing
coverslips from previously mounted 10 microns thick paraffin sections. In
addition, any comments on antigenicity left over on those sections, which were
previously stained with H&E?

************************************************************
*Cesar D. Fermin, Ph.D \|*|/ Fax (504) 587-7389 *
*Tulane Medical School /|*|\ Answ. Mach.(504) 584-2618 *
*Pathology/SL79 \|*|/ Secretary (504) 584-2436 *
*New Orleans, La 70 112 /|*|\ Lab (504) 5841 *
*Fermin-at-TMC.Tulane.edu -} Director of Morphological Services*
************************************************************





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Fri, 28 Apr 1995 9:10:20 -0500 (CDT)
Subject: Re: Image archive system

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From: cook-at-aaem.amc.anl.gov (Russell E. Cook)
Date: Fri, 28 Apr 1995 09:48:23 -0600
Subject: Re: Image archive system

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Message-Id: {v01510101abc6bcf603c9-at-[146.139.72.78]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

In response to Frank Scheltens query about the limits of the Mac OS to keep
track of files, our local computer systems administrator/guru, Paul
Domagala, said that the OS is limited to 65,000 per partition. That is,
each partition of a drive can have up to 65K files. He is unaware of any
other limitations. In other words, Frank, it appears that you won't have a
file-limitation problem, especially if most of the files are multi-megabyte
images. If you think you'll have too many files, just partition the
drives.




Russell E. Cook
Electron Microscopy Center for Materials Research
Argonne National Laboratory






From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Fri, 28 Apr 1995 8:58:59 -0500 (CDT)
Subject: S-SIMPLY : May update...

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From: epicier-at-univ-lyon1.fr (Thierry Epicier)
Date: Fri, 28 Apr 1995 10:55:18 +0200
Subject: S-SIMPLY : May update...

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A short message for users of (or people interested in) the freeware
version of 'S-SIMPLY' (SHRLI-SIMulation and diSPLaY of TEM
& HRTEM images, for PCs), available on FTP.UNIV-LYON1.FR
(/pub/dos/HRTEM) has been significantly re-updated, and new
features are available (graphical displays, a "Make Interface" tool,
"Beams" / Pendellosung plot....).

P.S. : S-SIMPLY will be available for use at the NCEM SummerSchool
1995 "Computer Simulation and Processing of HRTEM Images"
to be held June 26-30, 1995, at NCEM, Berkeley, CA.
(for information : Michael A. O'Keefe, MAOK-at-LBL.GOV)


With best regards,



______________________________________________

Thierry EPICIER
GEMPPM-502, INSA de Lyon,
69621 VILLEURBANNE, France
tel : (33) 72 43 84 94 (83 85)
FAX : (33) 72 43 85 28
Email : EPICIER-at-CISMSUN.UNIV-LYON1.FR





From: Elinor Solit :      cambrex-at-world.std.com
Date: Fri, 28 Apr 1995 14:08:00 +0059 (EDT)
Subject: Re: Polaroid Micrograph Contest???

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Tina,

In response to your question about the Polaroid Photomicrography Contest,
it is no longer held. Polaroid called the event off in 1994, I believe
for economic reasons. So we'll all have to live with the wonderful images
it produced during its life span.

Regards,

Ellie Solit
Director of Publication
The Microscope Book

On Thu, 27 Apr 1995, Nestor J. Zaluzec-Argonne Nat. Lab. wrote:

} From: SMTP%"tina-at-wana.pbrc.Hawaii.Edu" 27-APR-1995 14:43:51.17
} To: MICROSCOPY
} CC:
} Subj: Polaroid Micrograph Contest???
}
} Date: Thu, 27 Apr 1995 10:37:40 -1000 (HST)
} From: Tina Carvalho {tina-at-ahi.pbrc.Hawaii.Edu}
} Subject: Polaroid Micrograph Contest???
} To: Microscopy Newsgroup {microscopy-at-aaem.amc.anl.gov}
} Message-Id: {Pine.3.89.9504271047.A3193-0100000-at-ahi}
} Mime-Version: 1.0
} Content-Type: TEXT/PLAIN; charset=US-ASCII
}
} Does anyone know if there will be a Polaroid International Micrograph
} Contest this year? I've not been able to get hold of anyone at Polaroid
} who knows...
}
} Aloha,
} Tina Carvalho
} Biological EM Facility
} University of Hawaii
}




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Fri, 28 Apr 1995 9:11:09 -0500 (CDT)
Subject: Re: Image archive system

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From: DOUG ARRELL :      ARRELL-at-fs-iam-1
Date: Fri, 28 Apr 1995 08:22:45 GMT+0200
Subject: Re: Image archive system

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Message-Id: {MAILQUEUE-101.950428082245.352-at-FS-IAM-1.JRC.NL}

} Fellow Microscopists,
}
} In the process of designing an image archive system (Mac based/huge external
} HD/CD-ROM writer/CD-ROM Jukebox), I have heard rumblings concerning the
} limitations of the Mac OS with regard to the number of individual files and
} total external SCSI HD capacity that can be handled by the system. This
} could be a big problem; we are planning on handling thousand of MB size
} images on muli GB external drives before writing to the CD-ROM.
}
} Has anybody out there experienced problems of this type?
}
} Advise from anyone operating a similar system would be greatly appreciated!
}
}
} Cheers,
} Frank


MacOS up to version 7.1 was only capable of holding 2Gb per disk
partition. I don't think that you could easily produce multiple
partitions either, but I may be wrong here. System 7.5 allows disks
of up to 4Gb. To use larger disks you _may_ need to purchase some
disk partition software. We use 'Formatter Five' from Software
Architects which allows us to put multiple partitions (mixed Mac and
PC) on the same disk so that we can use the same removable disks on
Macs and PCs as transferring a few hundred Mb at a time is very slow
on our network.

Doug

******************************************
Dr Douglas Arrell
Mechanical Properties and Joining Division
Institute for Advanced Materials
Joint Research Centre
European Commission
Westerduinweg 2
1755 ZG Petten
The Netherlands

Tel. (+31) 2246 5287
Fax. (+31) 2246 1917
E-Mail Arrell-at-JRC.NL
*********************************




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Fri, 28 Apr 1995 9:03:46 -0500 (CDT)
Subject: REcycle....

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From: hunt-at-msc.cornell.edu
Date: Fri, 28 Apr 1995 09:19:57 -0400 (EDT)
Subject: Re: recycle silver paste?

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Reply-To: hunt-at-msc.cornell.edu

}
} From: SMTP%"tina-at-wana.pbrc.Hawaii.Edu" 26-APR-1995 14:44:05.69
} To: MICROSCOPY
} CC:
} Subj: recycle silver paste?
}
} Date: Wed, 26 Apr 1995 10:26:53 -1000 (HST)
} From: Tina Carvalho {tina-at-ahi.pbrc.Hawaii.Edu}
} Subject: recycle silver paste?
} To: Microscopy Newsgroup {microscopy-at-aaem.amc.anl.gov}
} Message-Id: {Pine.3.89.9504261052.A29751-0100000-at-ahi}
} Mime-Version: 1.0
} Content-Type: TEXT/PLAIN; charset=US-ASCII
}
} Aloha, all-
}
} I have some 150 grams of dried-up colloidal silver paste that is not worth
} trying to get back into solution, partly because it costs more to ship
} the solvent to Hawaii than to purchase new paste. Is it worth trying to
} recycle somehow? What all is in it besides silver? Is it pretty pure?
} I'm not even sure there's anyone in Hawaii who recycles silver from darkroom
} chemicals.
}
} Besides the osmium, what else should us environmentally-concerned
} microscopists be trying to conserve/recycle?
}
} A belated Happy Earth Day to you all!
}
} Mahalo,
} Tina Weatherby Carvalho
} Biological EM Facility
} University of Hawaii

Tina,
Mechanical vacuum pump oil to gas stations if nowhere else.
Any large film developer should have a system for recycling fixer (which contains the Ag removed from the film). We bought containers to store several gallons in until we bring to a store down the street. There is a small charge for that service. Unfor
tunately, the campus life safety group doesn't pick this up like they do other chemicals for proper disposal. We have them pick up used acetone and alcohol etc.} I know Hawaii is different in unexpected ways; i.e. the price of milk. Good Luck.
I still remember the first Earth Day when I went to Herald Square, and collected bumper stickers etc.
John Hunt





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sat, 29 Apr 1995 11:14:33 -0500 (CDT)
Subject: Validation of EDS Equipment

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From: SMTP%"kris-at-miat0.vein.hu" 28-APR-1995 01:47:53.25
To: MICROSCOPY
CC:
Subj: Validation of EDS Equipment

Date: Fri, 28 Apr 1995 09:43:21 -0500
Message-Id: {9504281443.AA09249-at-miat0.vein.hu}
X-Sender: kris-at-miat0.vein.hu
X-Mailer: Windows Eudora Version 1.4.4
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
To: Microscopy-at-aaem.amc.anl.gov
From: kris-at-miat0.vein.hu (Kovacs Kristof)
Subject: Validation of EDS Equipment

Dear Barbara:

I don't know too much about the FDA validation process but if I were you I
would do the following:

Have some good, approved standards e.g. from SPI Supplies in West Chester,
PA (e-mail:sp-supp-at-cerf.com) and do several quantitative analysis runs on
composite standards. Compare the results with the specified and approved
standard values and report the results of comparison.

My other suggestions are connected to the operation of the instrument. In
order to do this you have to have a good textbook dealing with EDS analysis,
or some laboratory workbook such as Scanning Electron Microscopy, X-Ray
Microanalysis, and Analytical Electron Microscopy by Charles E. Lyman et al.

1. Check the incoming vs. processed count rate. For a good instrument there
should not be much difference up to about several thousand incoming counts.

2. Check the resolution of the detector with with a pure metal standard (not
necessarily Mn), calculate it to Mn K alpha and see if it is in accordance
with the specification.

3. Check the window constant of the detector. It is important to know the
detector efficiency especially for light elements.

That's all, if you have any further question just send me a message.

Wish you good luck!

Kris

(kris-at-miat0.vein.hu)


End of returned message





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sat, 29 Apr 1995 11:15:51 -0500 (CDT)
Subject: Term lbs.....

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From: SMTP%"S.Helfer-at-rbge.org.uk" 28-APR-1995 02:28:33.15
To: ZALUZEC
CC:
Subj: Re: Term lbs.

To: "Nestor J. Zaluzec-Argonne Nat. Lab." {ZALUZEC-at-aaem.amc.anl.gov}
From: "STEPHAN HELFER" {S.Helfer-at-rbge.org.uk}
Organization: Royal Botanic Garden Edinburgh
Date: Fri, 28 Apr 1995 09:24:46 BST
Subject: Re: Term lbs.
Priority: normal
X-mailer: WinPMail v1.0 (R2)
Message-ID: {F09E282F0C-at-rbg-3.rbge.org.uk}

From: SMTP%"Campbell36-at-aol.com" 27-APR-1995 20:06:46.60
To: MICROSCOPY
CC:
Subj: Term lbs.


Can anyone tell me the origin of the term (lbs.) as it relates to the unit
of
measure pounds?

Thanks

Jim Campbell

Chambers Everyday Dictionary: Pound: unit of mass of varying value
[hence not metric] long used in Western and Central Europe, more or
less answering to the Roman LIBRA, whose symbol lb is used for
pound.. ... British pound = 0.54359237kg...

Incidentally the Pound Sterling used to have the value of 0.543...kg of
silver; comme ca change

Yours sincerely


Dr Stephan Helfer, SSO
Mycologist

Royal Botanic Garden Edinburgh, Inverleith Row, EDINBURGH EH3 5LR,
Scotland UK

email STEPHAN-at-rbge.org.uk
phone: +44 (0)131 552 7171 ext 280
or +44 (0)131 459 0446-280 (direct digital VoiceMail line)
fax: +44 (0)131 552 0382

End of returned message





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sat, 29 Apr 1995 11:23:33 -0500 (CDT)
Subject: CCDs, point spread function

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From: jfmjfm-at-umich.edu (John F. Mansfield)
Date: Fri, 28 Apr 1995 11:11:18 -0400
Subject: CCDs, point spread function

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X-Sender: jfmjfm-at-srvr5.engin.umich.edu
Message-Id: {v0211011aabc6b4a8cd8a-at-[141.212.196.22]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Instead of all this discussion on the dynamic range of CCDs, how about
considering the point spread function of scintillator/CCD systems?. If you
want to measure intensities, it seems to me that you should be taking this
into account.

Jfm


John Mansfield
North Campus Electron Microbeam Analysis Laboratory
413 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (313)936-3352 FAX (313)936-3352
jfmjfm-at-engin.umich.edu, John.F.Mansfield-at-umich.edu
or jfmjfm-at-umich.edu they all reach me!
URL: http://www.engin.umich.edu/~jfmjfm/jfmjfm.html






From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sat, 29 Apr 1995 11:24:31 -0500 (CDT)
Subject: Questions on Courses?

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From: hukee.margaret-at-mayo.EDU (Marge Hukee)
Date: Fri, 28 Apr 1995 11:14:40 +0200
Subject: Questions on Courses?

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Message-Id: {9504281612.AA17153-at-fermat.Mayo.EDU}
X-Sender: hukem-at-fermat.mayo.edu
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Two very quick questions:
Is a short course (1 week)in biological TEM available in the US?
Is Dr. Bendayan offering his course on colloidal gold labeling this year?
Thanks
Marge

Marge Hukee
EM Core Facility hukee.margaret-at-mayo.edu
Mayo Foundation (507) 284-3148
----------------------------------------------------------------------------
--






From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sat, 29 Apr 1995 11:26:46 -0500 (CDT)
Subject: FREE ZEISS TEM

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From: fsack-at-magnus.acs.ohio-state.edu
Date: Fri, 28 Apr 1995 10:36:16 -0400 (EDT)
Subject: excess tem

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X-NUPop-Charset: English

If you are interested in this TEM please contact Dr. Fred Sack directly.

M.V. Parthasarathy
------------------------------

Partha - we have a Zeiss 10 TEM in good shape that the College wants to
dispose of since they won't pay a service contract and moving charges (I
have my own but can't swing another one..)

Zeiss will take it back for free and we could strip it, but would be happy
to GIVE it away for anyone willing to pay for shipping. Do you know of
anyone interested??

Fred Sack phone: 614-292-0896
Plant Biology fax: 614-292-6345
Ohio State University e-mail: sack.1-at-osu.edu
1735 Neil Avenue alternate e-mail address:fsack-at-magnus.acs.ohio-state.edu
Columbus Ohio 43210




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sat, 29 Apr 1995 11:28:24 -0500 (CDT)
Subject: Cover Slip removal

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From: Peter D. Barnett :      pbarnett-at-crl.com
Date: Fri, 28 Apr 1995 13:26:23 -0700 (PDT)
Subject: Re: Coverslip removal

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} From: "Fermin, Cesar" {fermin-at-tmc.tulane.edu}
} Subject: FW: Coverslip removal
} To: "Zaluzec, Nestor" {ZALUZEC-at-AAEM.AMC.ANL.GOV}
} X-Mailer: Mail*Link SMTP-MS 3.0.2
}
}
}
} I will appreciate any trick (besides immersion in xylene) for removing
} coverslips from previously mounted 10 microns thick paraffin sections. In
} addition, any comments on antigenicity left over on those sections, which were
} previously stained with H&E?
}

Try freezing. I have had success by freeezing from an inverted can of
dust-off or similar compressed air product that uses a compressed gas as
a propellant. Direct the spray right onto the cover slip, the pop off
with a razor blade or scalpel blade.


Peter D. Barnett - Forensic Science Associates - Richmond CA
pbarnett-at-crl.com VOICE: 510-222-8883 FAX: 510-222-8887






From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sat, 29 Apr 1995 11:29:10 -0500 (CDT)
Subject: High quality video capture boards

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From: Alan Pooley :      pooley-at-ahab.rutgers.edu
Date: Fri, 28 Apr 1995 14:56:25 -0400
Subject: High quality video capture boards

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Can anyone with experience point me in the right direction toward
chosing a high resolution video capture board. We are about to buy a fuji
pictography 3000 color printer at $26,000. (Thanks to all who helped
me earier with advice about this and other printers). We are trying to
capture the highest possible spatial and color resolution from Betacam SP
tape via a Sony BVW 75 tape reader, which gives COMPONENT signal
(intensity Y, red-Y, Blue-Y). We hear various 'line' resolutions from
this, ranging from 450 to 750 analog lines of video resolution. Does anyone
know what the actual is? How does this translate into pixels? Do we need mo
Do we need more than 640 * 480 * 8 bits/color channel? Some of the
boards support 1024 * 1024 and various color resolution depths. Where
do you see a differnce? We are willing to go either powermac or pentium
and want to find the capture card with the best. We also want to be able
to capture 5-10 images 'on the fly' as the tape plays, rather
than slowwing it down or freezing the particular frame we want to capture.
The subject matter is color video of tube worm etc fields growing
rapidly at the hydrothermal vents as captured on betacam sp for national
geographic tv last november by Rich Lutz. Our purpose in digitizing
is both for measurement and for high quality image hard copy to the fuji
printer via photoshop on the powermac. (Does photoshop come on the PC?)

I would appreciate any help you can give me.

Alan Pooley Marine Science SEM & Morphometrics lab, Rutgers univ
908 932 8959 ext 225 pooley-at-ahab.rutgers.edu




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sat, 29 Apr 1995 11:30:15 -0500 (CDT)
Subject: More info on lbs...

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From: Chris Jefferies :      chris-at-stowey.demon.co.uk
Date: Fri, 28 Apr 1995 20:01:13 GMT
Subject: Re: Term lbs.

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Jim,

In your message dated Thursday 27, April 1995 you wrote :

} Can anyone tell me the origin of the term (lbs.) as it relates to the unit of
} measure pounds?

This is quite an interesting one, actually.

In Roman times the Latin work for a pair of scales or a balance was 'libra',
this word is still used for the astronomical constellation Libra, the scales.
Over time the word for scales became associated with a specific mass, what we
call a pound today.

The lb stands for 'libra', lbs is really a sort of false plural, adding an
Anglo-Saxon ending to a Latin word! 'Libras' is nonsense, it should have been
'librae' I suppose. That explains the lbs, but leaves the new question, 'Why did
we start to call the mass a 'pound', and when? Anyone know?

Oh, by the way. The English *monetary* value, the pound, was originally the
value of one pound in weight of metallic silver. Inflation has long since made
nonsense of that! But that explains the pound sign, it's an old-fashioned
copperplate capital 'L', for 'libra' again.

Kind regards,

Chris
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From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sat, 29 Apr 1995 11:31:37 -0500 (CDT)
Subject: Image

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From: gpma44-at-udcf.gla.ac.uk (Peter McHardy)
Date: Fri, 28 Apr 1995 18:05:26 +0200
Subject: Re: Image archive system

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Message-Id: {10094.199504281603-at-lenzie.cent.gla.ac.uk}
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From: gpma44-at-udcf.gla.ac.uk (Peter McHardy)
Date: Fri, 28 Apr 1995 18:05:26 +0200
Subject: Re: Image archive system

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external=20
} HD/CD-ROM writer/CD-ROM Jukebox), I have heard rumblings concerning the=20
} limitations of the Mac OS with regard to the number of individual files and=
=20
} total external SCSI HD capacity that can be handled by the system. This=20
} could be a big problem; we are planning on handling thousand of MB size=20
} images on muli GB external drives before writing to the CD-ROM.
}
} Has anybody out there experienced problems of this type?
}
} Advise from anyone operating a similar system would be greatly appreciated!=
=20
}
}
} Cheers,
} Frank
Dear Frank,
This is a problem that we are also facing. At the moment it appears=
=20
that we are collecting about 1.5 Gb of confocal images a month. On top of=20
this our network has also to deal with a large volume of images from other=
=20
sources such as laser scaning densitometers. Our network consists of a SUN=
=20
based PCNFS network with ~80 PCs and 10 MACs all acessing files held=20
centrally on our unix machine. All our file should ideally be available to=
=20
all machines and we also need to be sure that they are all properly backed=
=20
up in case of accidents.
I am currently talking to a number of computer suppliers regarding=
=20
data storage. The current favourite option appears to be to have a large=20
disk farm, about 27 Gb for online storage, then as this becomes fully to=20
move of data onto a read/write optical juke box then finally as this becomes=
=20
full to write CD roms. The price for this system complete with backup=20
software appears to be about =A320-30 sterling.=20
What I was reallly wondering is what do you other confocal=20
microscopist do? I am a microscopist and I don't want to spend all day being=
=20
a network manager so I am looking for a simple answer.


Peter



Peter McHardy,
Technology Services Manager,
Dept. Medical Oncology,
Glasgow University,
Alexander Stone Building,
Garscube Estate,
Switchback Road,
Bearsden,
Glasgow
G61 1BD
Tel No. 041-330 4811 Fax No. 041-330 4127
e-mail:gpma44-at-udcf.gla.ac.uk





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sat, 29 Apr 1995 11:33:19 -0500 (CDT)
Subject: Re-send of INTEGRATED MICROSCOPY SYMPOSIUM

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Subscriber's,

This message got accidently confused by me and
was incorrectly titled. My apologizes


Nestor.

---------------------


Meeting Announcement

Symposium on

"INTEGRATED MICROSCOPY"

September 29 to October 1, 1995

at

The Wisconsin Center
702 Langdon Street
Madison, WI 53706

organized by the

Integrated Microscopy Resource
University of Wisconsin-Madison

********************************************************************************
Presentations in this symposium will focus on biological problems for which
a combination of microscopies (i.e. integrated microscopy) has been used.
In this way the speakers will demonstrate by example the power, potential
and limitations of various microscopical techniques. The techniques which
will be discussed include: DIC, confocal, 2-photon excitation imaging,
SEM, TEM, cryo-specimen preparation, and AFM.
********************************************************************************

FRIDAY (Evening), September 29, 1995
6:00 - 7:55 RECEPTION
8:00 - 8:45 Ken Jacobson, Univ. of N. Carolina-Chapel Hill
8:50 - 9:35 John Sedat, Univ. of California-San Francisco
9:40 - 10:25 Edward Salmon, Univ. of N.Carolina-Chapel Hill
SATURDAY, September 30, 1995
8:30 - 9:15 D. Lansing Taylor, Carnegie-Mellon University
9:20 - 10:05 Jeff Lichtman, Washington University
10:10 - 10:40 COFFEE BREAK
10:45 - 11:30 John White, University of Wisconsin-Madison
11:35 - 12:20 Steven Smith, Stanford University
12:25 - 1:25 LUNCH (on your own)
1:30 - 2:15 Gary Borisy, University of Wisconsin-Madison
2:20 - 3:05 Ralph Albrecht, Univ. of Wisconsin-Madison
3:10 - 3:55 Paul Bridgman, Washington University-Madison
5:00 - 6:00 EXHIBITOR'S SOCIAL
6:00 - 8:00 BUFFET DINNER
SUNDAY (Morning), October 1, 1995
9:00 - 9:45 Kent McDonald, Univ. of California-Berkley
9:50 - 10:35 Stanley Erlandsen, University of Minnesota
10:40 - 11:10 COFFEE BREAK
11:15 - 12:00 Hans Ris, University of Wisconsin-Madison
12:05 - 12:50 Eric Henderson, Iowa State University


Fees:
General Registration $100.00 (Late fee: $130.00)
(Includes: Fri. Reception, Sat. Social & Dinner, Coffee
Breaks and Materials)
Local Registration $ 80.00 (Late fee: $110.00)
(Includes: Fri. Reception, Sat. Social, Coffee Breaks and
Materials)
Guest Ticket - Saturday Dinner $ 20.00 (On-site Unavailable)


Interested in attending?

Consult our Web site for additional information:

http://www.bocklabs.wisc.edu/imr/imr.html


To receive a brochure and registration form write to:
IMR
University of Wisconsin-Madison
1675 Observatory Drive
Madison, WI 53706

or email: imradmin-at-calshp.cals.wisc.edu



Following the symposium, there will be a two-day workshop at the IMR. We
will be presenting lectures and provide "hands-on" experience for the
following techniques:
* 2-photon excitation imaging
* 4D DIC imaging
* cryo-SEM
* high pressure freezing
* reversible embedment for SEM and TEM

Workshop attendence will be limited to 25 students. A letter of
application is required. Fee: $150.00.





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sat, 29 Apr 1995 11:34:51 -0500 (CDT)
Subject: greys levels, film vs digital

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From: jpawley-at-macc.wisc.edu (James Pawley)
Date: Fri, 28 Apr 1995 19:22:22 -0600
Subject: Re: greys levels, film vs digital

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Message-Id: {9504290005.AA13740-at-sage.macc.wisc.edu}
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Sorry to be late in seeing this discussion but, looking over the points
made so far, I think that there seems to be a misunderstanding.

Yes, the CCD part of the Gatan (or Teitz etc.) digital TEM image recording
system may have a dynamic range of 14 to 16 bits, however, there is not a
1-to-1 relation between electrons-in-the-beam and electrons-in-the-CCD.
The beam electrons are first converted to photons in some sort of phosphor
or scintillator. Although the exact number of photons
produced/incident-beam -electron varies with the energy of the beam (less
at higher kV) and with the thickness and type of phosphor, 500 might be a
good number.

Of these, only a small fraction make it to the CCD while others are lost
because they are going in the wrong direction to start off with and never
get out of the phosphor layer (because of total internal reflection,
scattering or absorbtion in the phosphor, either YAG-Ce or P-20) or because
they never get into the fiber-optical coupling (or lens system), as they do
not strike the lens or fiber-optic plate at the correct angle. As a result,
50-100 photons may reach the CCD, producing 25-50
electrons/incident-beam-electron. For this reason the dynamic range of the
entire detector is 25-50 times less for electron-optical use than for use
with light. One can use a thinner phosphor or a FO-plate with a lower NA
to reduce this factor but then you lose the single-electron sensitivity
that is also a good feature of this detector.

As mentioned by others, one of the other good features is the linearity of
response of this detector. However, one should remember that the response
of film to direct kV electron exposure is far less non-linear than is its
response to light. This is because it is generally believed that
two-photons are needed to expose a silver grain (and therefore the
blackening is proportional to intensity squared) whereas only a single
kV-electron is needed to expose a grain (because this single electron is
likely to deposit far more energy with each interaction.).

The dynamic range of film exposed to electrons is likewise less than the
linear exposure range (or the range of your digitizer) because each 100 kV
beam electron (though perhaps not each 1000 keV etectron) is likely to
expose (many) more than one grain (depending on grain size, emulsion
thickness and silver density). As a result, although one may be able to
digitize a brightness ratio or 10,000:1 from the developed film, this may
actually only represent an electron intensity range of 1,000:1 or less.

Because of its very low "fog" level, the Fugi imaging plate is (extremely)
linear over a larger range but the resolution is also low so one must
generally operate at a higher magnification (and hence a higher dose rate
to the specimen).

An excellent reference on the response (resolution and "speed") of a wide
variety of photographic emulsions to 100 keV electrons is given in K.H.
Downing and D.A. Grano, Ultramicroscopy 7:381 (1982)
}
} Thomas E. Phillips wants comments on grey levels possible in film vs.
} digital. Film response is a sigmoidal curve and one adjusts exposure to
} fit the light coming off the subject to the linear portion of the curve in
} the film's response. Black & white film provides a greater number of lens
} aperture stops (6-8) to capture greys compared to color film (about 4,
} depends on the film). So the answer to the question of grey values
} possible in film is that one can capture a great variety of greys in film,
} but not all in one image-only a relatively narrow range can be captured,
} with the appropriate adjustment of exposure. CCDs have a linear response
} over several orders of magnitude. Slow scan CCDs can capture a remarkable
} range of greys in each image, in which case one can choose which range of
} greys to display on the moniter.
}
}
}
}
} R. Howard Berg, Ph.D.
} Biology Department
} University of Memphis
} Memphis, TN, 38152
} E mail: bergrh-at-cc.memphis.edu
} phone: 901-678-4449 fax: 901-678-4457
}

***************NEW ADDRESS**************
Prof. James B Pawley, Ph. 608-263-3147
Room 1235, Engineering Research Building, FAX 608-265-5315
1500 Johnson Dr. Madison, Wisconsin, 53706.
JPAWLEY-at-MACC.WISC.EDU






From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sun, 30 Apr 1995 20:48:08 -0500 (CDT)
Subject: Restarting the Automatic Delivery Mode

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Morning Subscribers,

I am going to attempt a restart of the automatic
delivery mode tonight. Over the weekend we
had only slightly more than normal "error"
which generally were due to network connection
failures, computers shutdown, disk full problems.
Basically, nothing that looked like a loop,
or bouncing messages. Remember if a delivery
error occurs, the originator of the message will
get the report and I will only see it if the
originator's node is down. We continue to
have problems with some nodes shutting down & or crashing.
so bewarned that some delivery error messages
will virtually always appear. Generally much less
than 1% of the mail that comes thorough this system
is not delivered. Just remember that for every
single message that comes into the host over 2000 copies
leave.

Keep your collective fingers crossed.

Nestor
Your Friendly Neighborhood SysOp...





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sun, 30 Apr 1995 20:48:08 -0500 (CDT)
Subject: Restarting the Automatic Delivery Mode

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Morning Subscribers,

I am going to attempt a restart of the automatic
delivery mode tonight. Over the weekend we
had only slightly more than normal "error"
which generally were due to network connection
failures, computers shutdown, disk full problems.
Basically, nothing that looked like a loop,
or bouncing messages. Remember if a delivery
error occurs, the originator of the message will
get the report and I will only see it if the
originator's node is down. We continue to
have problems with some nodes shutting down & or crashing.
so bewarned that some delivery error messages
will virtually always appear. Generally much less
than 1% of the mail that comes thorough this system
is not delivered. Just remember that for every
single message that comes into the host over 2000 copies
leave.

Keep your collective fingers crossed.

Nestor
Your Friendly Neighborhood SysOp...





From: bob-at-befvax.uchicago.edu
Date: Sun, 30 Apr 1995 15:48:06 EDT
Subject: RE: CCDs, point spread function

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Indeed Mansfields remark is correct. The point spread function is the
definitive description of the performance of any detector. The
psf of CCD cameras is described by Kujawa and Krahl in Ultramicroscopy
46, 395-403 (1992) and by Daberkow et al Ibid 38 215-223 (1991).

Both papers are well written and lucid.


Robert Josephs
Univ of Chicago
312 702 -1077





From: Peter Steele :      70152.3105-at-compuserve.com
Date: 30 Apr 95 11:19:32 EDT
Subject: Coverslip removal - nitrogen

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Instead of compressed gases, we routinely use liquid nitrogen. Just hold the
slide in the 'fumes' until the coverslip goes opaque (you can try submersing for
10 s). The coverslip will pop off with gently prying from a single edge safety
razor blade.

As for antigenicity on H&E it is very much an individual trial an error. Start
by de-coloring followed by a 1 hour soak in buffer. Then proceed as normal.

Peter O. Steele, Ph.D.
All Children's Hospital
St. Petersburg, FL





From: m.dickson-at-unsw.edu.au (Melvyn R. Dickson)
Date: Mon, 1 May 1995 11:23:32
Subject: Re: Osmium oxidation

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To: microscopy-at-AAEM.AMC.ANL.GOV

Osmium solutions that have reduced (and changed color) can be partially or
wholly restored by reoxidation using hydrogen peroxide. Just add dilute
peroxide dropwise until the colour lightens. If the change is not too
advanced, the original oxidation state can be completely restored.

We saved up discarded osmium from around australia for years then sent it to
Johnson Matthey. But the concentration was so low the value of the product
only just covered the cost of reclamation and refining. We didn't make money
but I guess we got a warm fuzzy from saving a non renewable product

Mel Dickson




From: em-at-mediacity.com (E. Monberg)
Date: Sun, 30 Apr 1995 21:56:57 -0800
Subject: FS: NEW EYEPIECES 30mm BARRELS "10x/20" (fit Zeiss, Leitz, Wild)

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Message-Id: {m0s5nU0-000rdNC-at-easynet.com}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

FS: NEW EYEPIECES 30mm BARRELS "10x/20" (fit Zeiss, Leitz, Wild)

I have a few sets of some new, modern design microscope eyepieces with a
quite wide field of view, and fit ZEISS and LEITZ and WILD WF binocular
eyetubes. Also work quite well with astronomical optics.

"WF10x/20"
TUBE DIAMETER 30mm/1.18"
MFG B&L
MODEL 31-15-77
ORIGINAL COST $264/set
CONDITION New in original packaging

My Price $89/pair

Ed Monberg em-at-mediacity.com








From: em-at-mediacity.com (E. Monberg)
Date: Sun, 30 Apr 1995 21:56:53 -0800
Subject: Gray Levels,FS:EIKONIX (Kodak) 2048x2048x12 SCANNING CCD CAMERA

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Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

FS: EIKONIX (Kodak) 2048x2048x12 SCANNING CCD CAMERA

I have an Eikinix Model EC78/99 Camera system for sale.

It is designed for Medical Imaging, and other applications requiring good
intensity resolution, with a 12 bit (4000:1) grayscale.

Includes:
55mm Nikon Micro-Nikor Lens
Camera Unit
Control Unit, in short, the whole thing, and
The Manual.

Price ? Comparable units such as the Leaf, are LOTS of money. So wow me,
and give an offer you can't refuse, and neither can I.

Ed Monberg em-at-mediacity.com






From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Mon, 1 May 1995 9:10:57 -0500 (CDT)
Subject: Collodial Gold CytoChem Course

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From: Diane Montpetit :      montpetitd-at-EM.AGR.CA
Date: Mon, 01 May 1995 07:30:03 -0400
Subject: Questions on Courses? -REPONSE

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Message-Id: {sfa4924c.089-at-EM.AGR.CA}
X-Mailer: Novell GroupWise 4.1

Marge,

Dr Bendayan is offering a course on colloidal gold
cytochemistry and in situ hybridization, may 29th -june
2nd 1995. The fax number is 514 343 2459.

Diane Montpetit






From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Mon, 1 May 1995 9:11:30 -0500 (CDT)
Subject: More info on lbs...

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From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sat, 29 Apr 1995 11:30:15 -0500 (CDT)
Subject: More info on lbs...

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Message-ID: {n1412803875.14228-at-postoffice.tmc.tulane.edu}

Ref. Pound "lb"
Incidentally, libra is the Spanish word for pound. Every Spanish speaking
country uses it, and there are quite a few out there! This is a good example
of how other languages crip into the English language (remember the past
controversy about Spanish language messages?) wanted or not. Fortunate for
some of us (who knows more than just English, and actually welcome other
sounds and symbols) this is a just another "thing" not a problem.
________________________________________________________
To: MICROSCOPYLISTNESTOR-at-AAEM.AMC.ANL.GOV
Cc: ZALUZEC-at-AAEM.AMC.ANL.GOV




From: Chris Jefferies :      chris-at-stowey.demon.co.uk
Date: Fri, 28 Apr 1995 20:01:13 GMT
Subject: Re: Term lbs.

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Jim,

In your message dated Thursday 27, April 1995 you wrote :

} Can anyone tell me the origin of the term (lbs.) as it relates to the unit
of
} measure pounds?

This is quite an interesting one, actually.

In Roman times the Latin work for a pair of scales or a balance was 'libra',
this word is still used for the astronomical constellation Libra, the scales.
Over time the word for scales became associated with a specific mass, what we
call a pound today.

The lb stands for 'libra', lbs is really a sort of false plural, adding an
Anglo-Saxon ending to a Latin word! 'Libras' is nonsense, it should have been
'librae' I suppose. That explains the lbs, but leaves the new question, 'Why
did
we start to call the mass a 'pound', and when? Anyone know?

Oh, by the way. The English *monetary* value, the pound, was originally the
value of one pound in weight of metallic silver. Inflation has long since made

nonsense of that! But that explains the pound sign, it's an old-fashioned
copperplate capital 'L', for 'libra' again.

Kind regards,

Chris
---------------------------------------------------------------------------
| Chris Jefferies E-Mail at home - chris-at-stowey.demon.co.uk |
| at work - chris.jefferies-at-bbsrc.ac.uk |
| Microscopy page {URL: http://metro.turnpike.net/jefferie/index.html} |
---------------------------------------------------------------------------





From: bergrh-at-cc.memphis.edu (R. Howard Berg)
Date: Mon, 01 May 1995 12:39:39 +0600
Subject: RE: CCDs, point spread function

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Lest anyone get confused, the PSF is a function of aberrations in the
optical system, NOT a function of CCDs.


} Subject: RE: CCDs, point spread function
}
} Indeed Mansfields remark is correct. The point spread function is the
} definitive description of the performance of any detector. The
} psf of CCD cameras is described by Kujawa and Krahl in Ultramicroscopy
} 46, 395-403 (1992) and by Daberkow et al Ibid 38 215-223 (1991).
}
} Both papers are well written and lucid.
}
}
} Robert Josephs
} Univ of Chicago
} 312 702 -1077


R. Howard Berg, Ph.D.
Biology Department
University of Memphis
Memphis, TN, 38152
E mail: bergrh-at-cc.memphis.edu
phone: 901-678-4449 fax: 901-678-4457







From: Daniel L. Callahan :      dlc-at-owlnet.rice.edu
Date: Mon, 1 May 1995 12:49:14 -0500 (CDT)
Subject: looking for used SEM

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A colleague is looking for a used LaB6-compatible SEM for use in
constructing a SEMPA system. If anyone has an old instrument they don't
want, please drop me a note.

Daniel L. Callahan
Department of Mechanical Engg. and Materials Science
Rice University
dlc-at-owlnet.rice.edu




From: Michael OKeefe :      Michael_OKeefe-at-macmail7.lbl.gov
Date: 28 Apr 1995 10:17:06 U
Subject: D-19 at NCEM

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Message-Id: {n1413058171.28446-at-macmail7.lbl.gov}

Subject: Time:10:18 AM
OFFICE MEMO D-19 at NCEM Date:4/28/95


Frank Karl wrote ---
} Subject: Alternates to D-19

} Hi everyone!

} We are currently using Kodak D-19 developer and Kodak Electron
} Microscopy Film 4489. We typically develop 123 pieces of film before
} changing developer and due to the relatively large number of potential
} users (6 people) we burn through the stock solution of D-19. Mixing D-19
} from powder is time consuming activity, and in this era of doing more
} with less I would like to find a developer sold as a liquid concentrate.
} This would speed up darkroom maintenance and let us spend more time
} developing and less time preparing to develop.

} Can anyone recommend a liquid developer? And while I'm asking, is
} there a better or equivalent film than 4489?


} Thanks for your input.


} Frank Karl fskarl-at-goodyear.com
} Goodyear Tire & Rubber Co. Voice 216.796.7818
} Analytical Services - Dept 415B Fax 216.796.3304
} 142 Goodyear Blvd
} Akron, OH 44305
} U.S.A.

--------------------------------------
Frank,
At the National Center for Electron Microscopy, in Berkeley, we are
using Kodak SO-163 for all of our microscopes. Although this film
exhibits a slightly larger grain size, it has the developing behavior
of ordinary B+W film. SO-163 can be developed in virtually any
common developer on the market (as well as some uncommon ones
not on the market!). For those who practice high resolution TEM,
SO-163 can be given one third of the indicated exposure (to help reduce
drift) and push processed with good results. 4489 has none of these
added benefits. Another potential problem is the cadmium present in
4489. Kodak warns the the cadmium leaches out into the developer
during processing and could make the disposal of the developer a
more complicated matter.

We are developing all of our film in D-19, except convergent beam
diffraction patterns and some SAD patterns. For these we use a custom
developer to reveal fine detail and maintain a printable nagative.
We typically process 800 plates per gallon of D-19 (full strength).
We find very little change in film density after 800 plates, this
typically takes two weeks for each darkroom. Our limiting factor
is the amount of film the fixer can handle before it is exhausted.
We do mix our D-19 with de-ionized water. After 800 plates the
D-19 looks like dark scum but still works quite well. D-19 has a
large sodium sulphite content which acts as a preservative. This
is the most industrial strength developer known on the planet.
I know of no substitute in a liquid form with the staying power
of D-19.

John Turner, NCEM







From: tivol-at-orkney.ph.albany.edu
Date: Mon, 01 May 1995 15:39:14 EDT
Subject: Re: Residual Gas Analyser: req for info

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Dear John,
Our AEI EM7 hvem came with a couple of simple quad mass specs, and we
use them occasionally to see what remains in the accelerator or column. We
have found primarily water, N, O, and hydrocarbons (no LN2 cooling) or N and
O (with LN2 cooling), so the information is not especially useful to us.
However, if we develop a leak in the accelerator, there would be peaks
characteristic of SF6. In this case, the RGA would be very useful. Knock wood
we hope this never happens.
The equipment seems to be very robust and easy to use, but I don't
know whether the same kind of thing is available for your machine or whether
the intervening 20 years have seen significant improvements. Good luck.
Yours,
Bill Tivol




From: Bohdan Soltys :      soltysb-at-fhs.csu.McMaster.CA
Date: Mon, 1 May 1995 16:14:02 +0500 (EST)
Subject:

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subscribe microscopy

soltysb-at-fhs.mcmaster.ca








From: george.farrants-at-calidris-em.se (george farrants)
Date: Mon, 1 May 1995 22:01:24 +0200
Subject: Correct Email address

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In a recent Electron Crystallography Newsletter we announced a new E-mail
address. However, this turned out to be rather premature and due to
adminstrative problems we are unable to use the address we had been given.
So I've had to change E-mail address again, and the new address is

george.farrants-at-calidris-em.se

Please spread this address around.

George Farrants
Calidris
Manhemsvagen 4
S-191 45 Sollentuna
Sweden
Tel and fax: +46 8 625 0041






From: dr Keller Eva :      KELEVA-at-igaz.sote.hu
Date: Tue, 2 May 1995 07:24:33 +100
Subject: subscription request

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Hello,

I would like to join to the micrscopy email.
keleva-at-sote.igaz.hu

Thanks:
Eva Keller




From: BILL RHOTEN :      RHOTEN-at-musom01.mu.wvnet.edu
Date: Tue, 02 May 1995 08:17:42 +1100
Subject: unsubscribe

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please unsubscribe
Its been grand!




From: YMEW34A-at-prodigy.com (MR DAVE A GRUEN)
Date: Tue, 02 May 1995 13:42:14 EDT
Subject: Subscribe microscopy YMEW34A@prodigy.com

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Subscribe microscopy YMEW34A-at-prodigy.com





From: troczyns-at-unixg.ubc.ca (Tom Troczynski)
Date: Tue, 2 May 1995 11:54:29 -0700
Subject:

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unsubscribe





From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 1 May 1995 16:04:52 -0400
Subject: RE-Silver Paint

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Message-ID: {n1412778020.91088-at-mse.engin.umich.edu}

Subject: Time: 3:53 PM
OFFICE MEMO RE:Silver Paint Date: 5/1/95

I have had moderate quantities of silver paint dry out on a number
of occasions, and have been able to restore them to useful form
simply by pouring a mixture (about 50-50) of acetone and amyl
acetate (a common constituent of fingernail polish remover)
into the container, sealing it up again, setting it to one side on my
desk and shaking it whenever I notice it. It takes a few days
to redissolve silver paint that has really dried out, but it can
be done quite satisfactorily if you give the solvent enough
time to act. Neither of the solvents involved should be
particularly rare in Hawaii.
Based on this performance, I would 'guess' that in
addition to the silver powder and the solvent, silver paint
also contains a bit of a binder material that is probably
akin to ordinary household cement. Good luck!





From: randy nessler :      rnessler-at-emiris.iaf.uiowa.edu
Date: Tue, 2 May 1995 08:46:30 -0500 (CDT)
Subject: 4pi Analysis, Inc. address

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Message-ID: {9FFEA52F0179AEAA-at-ggpl.arsusda.gov}


I am seeking the address and telephone # of 4pi Analysis, Inc. I
see that they are sustaining members of MSA, but have yet to see a ad
featuring their address or number. If anyone can help me, I would be
grateful.
Sincerely,
Randy Nessler
rnessler-at-emiris.iaf.uiowa.edu






From: Robert Fisher :      rmfisher-at-u.washington.edu
Date: Tue, 2 May 1995 10:57:20 -0700 (PDT)
Subject: Help Needed - FTIR MIcroscopy

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X-Sender: rmfisher-at-homer20.u.washington.edu


We are reactivating a BioRad FST-40 FTIR
microscope. No-one familiar with this instrument
seems to be around anymore.

We would like to make contact with an experienced
hands-on user of this or perhaps another model
of an FTIR scope.

A trip to Seattle and modest honorarium might be
arranged for someone who can help us.

Thanks

Bob Fisher





From: tphillips-at-biosci.mbp.missouri.edu (Tom Phillips)
Date: Tue, 2 May 1995 16:15:04 -0500
Subject: how thick of sections on ultramicrotome?

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I routinely cut 0.9 um semi-thin sections on my Reichert UltraCutE
but now I have a user who wants to use the Core Facility Reichert to cut 5
um thick JB-4 sections. I am a little nervous about this damaging the
ultramicrotome. Does anybody out there have any experience along these
lines? Thanks.



Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility
3 Tucker Hall
University of Missouri
Columbia, MO 65211
(314)-882-4712 (voice)
(314)-882-0123 (fax)






From: shamina :      s.rahman-at-rpms.ac.uk
Date: Wed, 3 May 1995 17:44:07 +0100 (BST)
Subject: unsubscribe

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Please could you unsubscribe me from your mailing list.

Shamina





From: Ciara Mullan :      mullanc-at-mcmail.CIS.McMaster.CA
Date: Tue, 2 May 1995 09:33:57 +0059 (EDT)
Subject: Re: Validation of EDS Equipment (fwd)

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I cannot remember if the original message was requesting information
about an EDS detector on an SEM or TEM, but we have been testing our new
detector (attached to our TEM) with a NiO test specimen. We
obtained a "kit" comprising a NiO test sample and instructions to
determine parameters such as energy resolution, peak to background ratios,
effect of electron optics, etc,etc. The reference for this system is
Egerton and Cheng, Ultramicroscopy 55(1994), 43-54. It is simple to use
and costs about 120-130$ (I am quoting Canadian dollars) from a subsiduary
of JEOL whose name has temporarily escaped me. If you wish to know more
you could contact me here
Hope this is of some help
Ciara






From: tphillips-at-biosci.mbp.missouri.edu (Tom Phillips)
Date: Tue, 2 May 1995 11:43:06 -0500
Subject: aldehyde fixes & fume hoods.

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I make and use my paraformaldehyde/glutaraldehyde fixes in the fume hood
but always take it out of the hood to pH with the meter on the other side
of my lab. I have always assumed this brief exposure was unavoidable.
Recently a colleague said he put his pH meter in the hood to avoid this.
Is this standard practice or is he simply being exceedingly careful? In
the old days, I remember everybody perfusing animals on the lab bench with
100's mls of fix. I would appreciate hearing how cautious you all are
with these fixes nowadays.

Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility
3 Tucker Hall
University of Missouri
Columbia, MO 65211
(314)-882-4712 (voice)
(314)-882-0123 (fax)






From: MicroToday-at-aol.com
Date: Wed, 3 May 1995 11:27:54 -0400
Subject: Biomedicine Atlas

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"Cell and Tissue Ultrastructure" by Patricia Cross and K. Lynne Mercer (Dept.
of Cell Biology, Stanford Univ. School of Medicine) describes the
ultrastructure of most cells of the body and how each structure relates to
function.
Some 180 cells are covered in an atlas format, with full page electron
micrographs on right-hand pages and corresponding text on facing pages.
The book was designed to combine the clarity of an atlas with the perspective
of an up-to-date cell biology text.
Available from Microscopy Today at $42.00 plus S&H: $7 for U.S.,
$9 for Canada, $10 for Mexico and $20 for other overseas.
Don Grimes, Microscopy Today





From: Miguel Avalos B. :      miguel-at-ifuname.ifisicaen.unam.mx
Date: Wed, 3 May 1995 16:27:14 -0700
Subject: Re: unsubscribe

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5













From: randy nessler :      rnessler-at-emiris.iaf.uiowa.edu
Date: Wed, 3 May 1995 08:11:53 -0500 (CDT)
Subject: Re: 4pi Analysis, Inc. address

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Wow,
It seems that I was the only person to not know their address.
Thanks to everyone who responded to my original post. The comments in
regards to customer satisfaction were also appreciated. I can now send
my letter requesting information.
Thanks again,
Randy Nessler
rnessler-at-emiris.iaf.uiowa.edu







From: Marcelle A Gillott
Date: 5/1/95 3:31 PM
Subject: Re: D-19 at NCEM

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Message-Id: {n1412777775.94908-at-macmail7.lbl.gov}
"Microscopy" {Microscopy-at-aaem.amc.anl.gov}
X-Mailer: Mail*Link SMTP/QM 3.0.0

Reply to: RE} } D-19 at NCEM

Dear Marcelle,
I passed yr msg on to John Turner (our photographer) , and he said --

We develop most of our conventional (non-HREM) negatives for four minutes at 20
deg. C. We agitate the negs once a minute by lifting the rack of film up and
down. It seems that this type of agitation gives more uniform results than
nitrogen burst. With nitrogen burst we found streaks along the film in areas
located close to a bubble opening in the tank.

Mike O'Keefe
--------------------------------------

Dear Michael

At full strength D-19, how long do you develop your negs?

thanks

Marcelle Gillott
UWM







From: EMLAB-at-opus.mco.edu
Date: Wed, 03 May 1995 08:55:38 -0500 (EST)
Subject: Re: Staining problems TEM

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Dear Charlie,

In my lab we use a saturated solution of uranyl acetate (aq) pH 3.3 for
15 min. followed by Renyolds lead citrate for 3 min. We always embed in
Spurr's. What type of samples? Cell culture monolayers stain less intense
than solid tissue. What is the molarity of your fixing buffer? Is the molarity
to low so that you are leaching out cytoplasmic material?
What KV, what size objective aperature are you using? The lower the
KV, the smaller the aperature the greater the contrast.

Hope these suggestions help,

Ed Calomeni
Medical College of Ohio
Toledo, OH
emlab-at-opus.mco.edu




From: MICROSCOPY-at-AAEM.AMC.ANL.GOV
Date: Thu, 4 May 1995 7:12:42 -0500 (CDT)
Subject: Help with EMPA of Minerals

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From: sysop-at-pfzrfsg.com (Michael Wallach)
Date: Mon, 1 May 1995 22:44:28 -0400
Subject: Help with EMPA of Minerals

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This message below was posted to sci.res. I have sent him JEOL and Links
addresses. If anyone has info on Bence Albee or other information handy
could you please send it directly to him.

Also, I am collecting infomation on microscopy and image analysis resources
on the Internet. The information will be available at a web site for
everyone and on disk and hard copy at EMSA (for a small fee that will cover
costs and FREE to those that contribute).

Thanks

Susanne Pignolet-Brandom, Ph.D.
MicroWorld Resources and News
708-548-6522



}
} I am trying to get information about the equipment listed below. The
} address/phone number of the manufacturer would be appreciated. I am also
interested in any literature references regarding its use for the analysis of
minerals.
}
} Quantitave Electron Probe Micro-Analysis (QEPMA) consisting of:
} JEOL JXA 8600 Superprobe
} Link Analytical AN 10/85S X-ray analysis system
}
} --- Via Silver Xpress V4.02B03 PFI-12521
}
} -----------------------------------------------------------------------
------
} Email: sysop-at-pfzrfsg.com (Michael Wallach)
} Pfizer Food Science Group, New York
} -----------------------------------------------------------------------




From: ANLAEM::MICROSCOPY 4-MAY-1995 07:14:47.07
Date: Thu, 4 May 1995 7:27:12 -0500 (CDT)
Subject: Staining problems TEM

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From: sfzane-at-unccvm.uncc.edu (Sandra F. Zane)
Date: Wed, 3 May 1995 09:38:03 -0400
Subject: Re: Staining problems TEM

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} I am having problems with certain specimens and their staining with uranyl
aceta
} te and lead citrate. The specimens
} do not take up much of the stain. They do not have alot of contrast. I
have cha
} nged the length of staining time , the
} [ ] of the stains, used ETOH with UA. The samples are well fixed in 2.5% glut.
} with ruthenium red, post fixed in
} OsO4, and embedded in spurrs.
}
}
} Thanks for any advice you may offer,
} Charlie Murphy

Good Morning Charlie,
It's not too unusual to have problems when staining specimens embedded
in Spurrs. I use 8% aqueous UrAc for an hour followed by Reynold's lead
citrate for 20 min. If you mix the UrAc with a stir bar for several hours
you can get it all to go into solution. You will, however, find that some
will settle out. Just take care not to stir it up before you use it. I also
filter both stains before use through a Swinnex filter unit fitted with a
Millipore 0.22uM filter.
I hope this is helpful.
Sandra Zane
Sandra F. Zane, EM Tech.
Biol. Dept. UNCC
Charlotte, NC 28223
sfzane-at-unccvm.uncc.edu
Fax (704) 547-3128





From: MAILER-DAEMON-at-INRS-ENER.UQuebec.CA
Date: Thu, 4 May 1995 09:27:34 -0400
Subject: Returned mail: Insufficient permission

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From: ANLAEM::MICROSCOPY 4-MAY-1995 07:14:56.64
Date: Thu, 4 May 1995 7:27:39 -0500 (CDT)
Subject: TEM STAINING

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From: SGKCCK-at-aol.com
Date: Wed, 3 May 1995 05:11:09 -0400
Subject: TEM STAINING

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Dear Mr. Murphy,
Regarding your posting about the above mentioned subject of problems with
contrast while using u.a. and l.c. on certain tissue only please note the
following:
When using ruthenium red and osmium maximum contrast is obtained when a
chloride free cacodylate buffer is employed(4% glut, 0.2M cacodylaye pH 7.3).
Keep in mind poor results may occur if the tissue is exposed to ruthenium
after primary fixation. It should further be noted that when using ruthenium
and osmium on certain tissues only you can get a decrease in contrast
especially if embedding in the epon family. To overcome these problems on
certain samples you can try vascular perfusion and as well you may want to
consider changing your embedding media to a water miscible one for these
specific tissues only.
For more info on this subject as well as suggested protocols see M.A.
Hayat(1993) Stains and Cytochemical methods Pg. 315-318.
For further infor please do not hesitate to contact me. Good luck
Stacie Kirsch
Electron Microscopy Sciences
P.O. Box 251
Fort Washington, Pa. 19034
Tel: 215-646-1566




From: ANLAEM::MICROSCOPY 4-MAY-1995 07:15:17.29
Date: Thu, 4 May 1995 7:28:18 -0500 (CDT)
Subject: TEM STAINING

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From: M.V. Parthasarathy :      mvp2-at-cornell.edu
Date: Wed, 3 May 1995 08:05:54 -0400 (EDT)
Subject: RE: Staining problems TEM

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X-NUPop-Charset: English

In message Tue, 2 May 95 09:42:55 -0400,
"Charlie Murphy, EML, B-177b" {cmurphy-at-ggpl.arsusda.gov} writes:

} I am having problems with certain specimens and their staining with
} uranyl acetate and lead citrate. The specimens do not take up much of
*************

Some of the plant material embedded in Spurr (especially the hard
formulation) can yield low contrast sections even after staining.
Have you tried staining in 5% ethanolic (50-70%) uranyl acetate for
30 mins or more?

*************************************************************************
M.V. Parthasarathy
Professor of Plant Biology & Director, EM Facility
Section of Plant Biology
228 Plant Science Building
Cornell University, Ithaca, NY 14850
Telephone: (607) 255-1734
Fax: (607) 255-5407
E-mail: mvp2-at-cornell.edu
***********************************************************************




From: Michael Cammer :      cammer-at-aecom.yu.edu
Date: Thu, 4 May 1995 10:47:47 -0400 (EDT)
Subject: shutters, filter wheels, and thanks for query responses

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I just found notes from the ASCB meeting in December in which I noted that
EMPIX filter wheels and shutter looked good and cheap. I judged the
software as weak, but liked that the hardware appeared to be NIH-Image
compatible-- the people demoing it claimed that simple commands via serial
port could be used-- and the price. Email address on literature:
empix-at-accesspt.north.net or traditional voice contact via (905) 542-8900.

At the end of March we posted a query regarding a purchase of software,
video & computer equipment, etc. Thank you for your responses; they were
helpful. We are now evaluating systems.

-Michael Cammer






From: SGKCCK-at-aol.com
Date: Thu, 4 May 1995 12:44:47 -0400
Subject: ALDEHYDE FIXES AND FUME HOODS

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Dear Dr. Phillips,

Regarding the above mentioned posting please noe that one can never be to
cautios but with all of this said at percentages of probably 2 or 3% of the
glut and form. I do not really see too much of a risk if you take its pH
outside of the fumehood. If you desire and you do want to be extremely
careful there are pentype portable pH meters that you can use in the fumehood
therefore never having to remove the chemical from the hood. The choice is
yours.
Good luck
Stacie Kirsch




From: Joyce Craig :      bafpjec-at-uxa.ecn.bgu.edu
Date: Thu, 4 May 1995 12:36:54 -0500 (CDT)
Subject: computers and microscopy workshop

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**





From: ANLAEM::MICROSCOPY 4-MAY-1995 07:15:49.97
Date: Thu, 4 May 1995 7:29:13 -0500 (CDT)
Subject: Re:seeking advice on starting a new career

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From: Melcom Copeland 225-1959 :      COPELAND.MELCOM-at-A1GW.GENE.COM
Date: Tue, 02 May 1995 00:05:00 -0800 (PST)
Subject: Re:seeking advice on starting a new career

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MR-Received: by mta MENDEL; Relayed; Tue, 02 May 1995 00:37:33 -0800
Alternate-recipient: prohibited
Disclose-recipients: prohibited


How should I get started in a new,exciting career in photography of
the microbial world?

My interest is in LM/TEM/SEM micrograhy and computer imaging. Would it
be best if I seek an advance degree in cell and molecular biology or
microbiology? Or should I go to art school and study photography? what
are going to be some of the prospective routes for me to take? Are
there any current training programs specific to this area of work? I'd
like to know.

I currently hold a B.A. in Biological Sciences, and have spent one
year in research doing in vivo pharmacology/toxicolgy since finishing my
undergraduate studies.

If you have any adivce I could use to start off my search i'd greatly
appreciate it. i'd love to receive any brochures or literature on
academic programs globally.

thanks,

melcom copeland e-mail: copeland.melcom-at-gene.com

address: 32602 Muirwood Dr.
Union City, California 94587






From: ANLAEM::MICROSCOPY 4-MAY-1995 07:16:18.95
Date: Thu, 4 May 1995 7:29:54 -0500 (CDT)
Subject: Staining problems TEM

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From: gwerdos-at-gnv.ifas.ufl.edu (Greg Erdos)
Date: Wed, 03 May 1995 08:46:08 -0500 (EST)
Subject: Re: Staining problems TEM

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} I am having problems with certain specimens and their staining with uranyl
acetate and lead citrate. The specimens
} do not take up much of the stain. They do not have alot of contrast. I
have changed the length of staining time , the
} [ ] of the stains, used ETOH with UA. The samples are well fixed in 2.5%
glut. with ruthenium red, post fixed in
} OsO4, and embedded in spurrs.
}
}
} Thanks for any advice you may offer,
} Charlie Murphy
}
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at- We find that we get much
better contrast using Epon (or its equivalent) or a 50:50 mix of Epon and
Spurrs ( see Bozzola & Russell, p. 26). LR White is another alternative,
which accepts staining quite well.
Pre-embedding staining with Ur is also helpful either in water after
Os or in 75% EtOH.. Just be sure you are phosphate free before using the Ur.
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Greg Erdos Phone: 904-392-1295
Scientific Director, ICBR EMCL
218 Carr Hall Fax 904-846-0251
University of Florida E-mail: gwerdos-at-gnv.ifas.ufl.edu
Gainesville, FL 32611





From: ANLAEM::MICROSCOPY 4-MAY-1995 07:16:28.01
Date: Thu, 4 May 1995 7:30:12 -0500 (CDT)
Subject: Staining problems

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From: m.dickson-at-unsw.edu.au (Melvyn R. Dickson)
Date: Thu, 4 May 1995 11:04:19
Subject: Staining problems

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To: microscopy-at-AAEM.AMC.ANL.GOV

. Zaluzec-Argonne Nat. Lab." {ZALUZEC-at-AAEM.AMC.ANL.GOV}
Poor staining can be helped in various ways:

1. You need good fixation with osmium first BUT with very long osmium
treatment too much material gets leached out. I reckon osmium needs to be in
contact with the tissue for around 2 hours, not including time for diffusion
through thick tissue or impermeable barriers

2. You can warm up the staining process. Do it in a 60 deg oven or under a
tungsten desk lamp. Put a petri dish cover over everything and put a water
soaked filterpaper under the cover to keep things from drying out.

3. You can stain overnight.

4. You can try a microwave burst

5. If you are mostly interested in membranes, fix with potassium permanganate
only (it dissolves everything else, but gives great contrast)

6. Lead stain in particular is unpredicatable. A bottle that works well can
stop working for no reason. A fresh solution may never work although the
recipe was followed exactly. Its like witchcraft.

7. Different buffers used with fixation have different effects on staining.
Phosphate is said to give best results (needs thorough washing before changing
to uranyl acetate) Cacodylate gives the next best.

8. I assume you are post fixing in uranyl acetate. You can extend a post fix
for several days if you are desperate.

Read Terzakis 1968 "Uranyl acetate a stain and a fixative" J Ultrastructure
Res vol 22 p 168.




From: ANLAEM::MICROSCOPY 4-MAY-1995 07:16:39.61
Date: Thu, 4 May 1995 7:30:31 -0500 (CDT)
Subject: Re: how thick of sections on ultramicrotome?

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From: Jay Jerome :      jjerome-at-isnet.is.wfu.edu
Date: Wed, 3 May 1995 14:36:52 -0400 (EDT)
Subject: Re: how thick of sections on ultramicrotome?

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For our intermediate voltage electron microscope we routinely cut 1-3 um
thick sections and have even gone as high as 5 um on some material. This
has not harmed our Ultracut E. However, we cut on diamond or glass knives
with specimen faces about 0.25-1 mm along the bottom edge. This is not
exactly a JB-4 type section, which tends to be bigger. If that is what
they want, a used JB-4 is not terribly expensive. Without evidence that
it will work (which you obviously are trying to obtain) I wouldn't risk
my microtome.


Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-isnet.is.wfu.edu *
**************************************************************

On Tue, 2 May 1995, Tom Phillips wrote:

} I routinely cut 0.9 um semi-thin sections on my Reichert UltraCutE
} but now I have a user who wants to use the Core Facility Reichert to cut 5
} um thick JB-4 sections. I am a little nervous about this damaging the
} ultramicrotome. Does anybody out there have any experience along these
} lines? Thanks.
}
}
}
} Thomas E. Phillips, Ph.D.
} Associate Professor of Biological Sciences
} Director, Molecular Cytology Core Facility
} 3 Tucker Hall
} University of Missouri
} Columbia, MO 65211
} (314)-882-4712 (voice)
} (314)-882-0123 (fax)
}
}
}




From: ANLAEM::MICROSCOPY 4-MAY-1995 07:17:04.29
Date: Thu, 4 May 1995 7:30:59 -0500 (CDT)
Subject: RGA's for electron microscopes

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From: Ostertag Tom :      ostertag_tom-at-mn15-gw.mavd.honeywell.com
Date: 5 May 1995 09:27:30 U
Subject: Index Fluids

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Message-Id: {9505041113.AA22999-at-riker.ml.wpafb.af.mil}

Microscopy List Participants:

I need to contact Cargille about purchasing a quantity of an index matching
fluid and I cannot find my catalog. Could someone please send me Cargille's
phone number.

Thanks,

Tom Ostertag
ostertag_tom-at-mn15-gw.mavd.honeywell.com
Tom.Ostertag-at-mavdmh.honeywell.com
tostertag-at-tcm.mn.org
Minneapolis, MN





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sun, 7 May 1995 18:12:53 -0500 (CDT)
Subject: shutters, filter wheels, and thanks for query responses

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From: Michael Cammer :      cammer-at-aecom.yu.edu
Date: Thu, 4 May 1995 10:47:47 -0400 (EDT)
Subject: shutters, filter wheels, and thanks for query responses

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I just found notes from the ASCB meeting in December in which I noted that
EMPIX filter wheels and shutter looked good and cheap. I judged the
software as weak, but liked that the hardware appeared to be NIH-Image
compatible-- the people demoing it claimed that simple commands via serial
port could be used-- and the price. Email address on literature:
empix-at-accesspt.north.net or traditional voice contact via (905) 542-8900.

At the end of March we posted a query regarding a purchase of software,
video & computer equipment, etc. Thank you for your responses; they were
helpful. We are now evaluating systems.

-Michael Cammer






From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sun, 7 May 1995 18:15:22 -0500 (CDT)
Subject: Validation of EDS Equipment (fwd)

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From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sun, 7 May 1995 18:25:15 -0500 (CDT)
Subject: Index Fluids

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Message-Id: {199505041723.NAA06523-at-titian.jeol.com}




From: Ostertag Tom :      ostertag_tom-at-mn15-gw.mavd.honeywell.com
Date: 5 May 1995 09:27:30 U
Subject: Index Fluids

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Microscopy List Participants:

I need to contact Cargille about purchasing a quantity of an index matching
fluid and I cannot find my catalog. Could someone please send me Cargille's
phone number.

Thanks,

Tom Ostertag
ostertag_tom-at-mn15-gw.mavd.honeywell.com
Tom.Ostertag-at-mavdmh.honeywell.com
tostertag-at-tcm.mn.org
Minneapolis, MN





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sun, 7 May 1995 18:26:56 -0500 (CDT)
Subject: absorption jump ratio

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From: Po-Fu Huang :      huang020-at-maroon.tc.umn.edu
Date: Fri, 5 May 1995 10:58:28 -0500 (CDT)
Subject: absorption jump ratio

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Hi, there,

Can anybody tell me what the definition of the absorption jump ratio is?
Thanks in advance.

Po-Fu Huang
huang020-at-maroon.tc.umn.edu




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sun, 7 May 1995 18:33:06 -0500 (CDT)
Subject: INDEX FLUIDS

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From: SGKCCK-at-aol.com
Date: Sat, 6 May 1995 10:17:29 -0400
Subject: INDEX FLUIDS

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Dear Tom,
I saw your message regarding the phone number of Cargille labs. There number
is 201-239-6633 in New Jersey.
If I can be of any further assistance please do not hesitate to call me.
Stacie Kirsch
P.O.Box 251
Fort Washington, Pa. 19034
Electron Microscopy Sciences
215-646-1566




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sun, 7 May 1995 18:32:01 -0500 (CDT)
Subject: Fwd: ALDEHYDE FIXES AND FUME ...

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From: SGKCCK
Date: 95-05-04 12:44:44 EDT
Subject: Fwd: ALDEHYDE FIXES AND FUME ...

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---------------------
Forwarded message:
Subj: ALDEHYDE FIXES AND FUME HOODS

Dear Dr. Phillips,

Regarding the above mentioned posting please noe that one can never be to
cautios but with all of this said at percentages of probably 2 or 3% of the
glut and form. I do not really see too much of a risk if you take its pH
outside of the fumehood. If you desire and you do want to be extremely
careful there are pentype portable pH meters that you can use in the fumehood
therefore never having to remove the chemical from the hood. The choice is
yours.
Good luck
Stacie Kirsch




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sun, 7 May 1995 18:15:52 -0500 (CDT)
Subject: aldehyde fixes & fume hoods.

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From: Richard E. Edelmann :      EDELMARE-at-CASMAIL.MUOHIO.EDU
Date: Thu, 4 May 1995 16:23:58 -500
Subject: RE: aldehyde fixes & fume hoods.

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Pen-type, i.e. handheld, pH meters conviently allow for checking of
the pH of nasties within the confines of a fume hood with out
cluttering up the limited space and air flow of a fume hood with an
expensive tabletop pH meter. Alternately, long lead wires on a table
to pH meter probe could also be used. Or working with the fix in a
flask does reduce the exit area for vapors from the fixitive solution
for quick pH measure measurment outside of the hood, but even this
allows some fixitive to escape as we can always still smell the fix
even with such a brief exposure.

The question still comes down to how much exposure is too much
exposure? Med school students still spend many hours hunched over
formaldehyde 'fixed' (yeah right) cadavers, and high school /
undergraduate students still spend hours chopping up formaldehyde
pickled worms, frogs, pigs, etc.. All without apropriate filtration
masks. So where does the answer lie?


Richard E. Edelmann
Electron Microscopy Facility Supervisor
352 Biological Science Building
Miami University, Oxford, OH 45056
Ph: 513-529-5712
E-mail: edelmare-at-muohio.edu




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sun, 7 May 1995 18:20:46 -0500 (CDT)
Subject: RE: aldehyde fixes & fume hoods.

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From: MR RHM CROSS :      EURC-at-giraffe.ru.ac.za
Date: Fri, 5 May 1995 08:03:18 GMT+0200
Subject: Re:seeking advice on starting a new career

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Hi!

We have recently embarked on an extensive program network linking
electron microscopes and light microscopes with a print & view
workstation running comprehensive image processing, archiving and
analysis facilities. From our experience setting this up I have
absolutely no doubt that your best career direction today would be in
the direction of computer-assisted imaging, etc. Your B qualification
in biological sciences will be enough in that direction for the time-
being (you will pick up new knowledge along the way in any case).
What you will need, however, is knowledge of image processing and
analysis.

Best of luck - it's a fascinating field and I wish I was young enough
to be in your position today.

Robin Cross
Director, Electron Microscopy Unit, Rhodes University, Grahamstown,
South Africa.




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sun, 7 May 1995 18:29:57 -0500 (CDT)
Subject: Light Microscopy Course

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From: spignole-at-ix.netcom.com (Susanne Brandom)
Date: Fri, 5 May 1995 19:37:47 -0700
Subject: Light Microscopy Course

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MICRO VISION ONE
Light Microscopy Course

June 19-23
Technical Education Center, Osceola, FL

Micro Vision One is an intensive week-long course on light microscopy,
designed to meet the requirements of both the new comer to microscopy
and the current practitioner who needs to become more versed in
specific areas. Built around a wide array of imaging and sample
preparation techniques, it will broaden microscopy skills for anyone
involved in the chemical, geological, or biological lab and well as
those in materials science, environmental or medical facilities.

Integrated lectures and laboratory assignments emphasize both the
microscopy techniques and the development of strategies for solving
every day problems. The course notebook includes key solutions which
illustrate those solutions and serves as a permanent reference manual

The course is taught by Barbara Foster of Microscopy, Marketing and
Education and Barry Gordon Fookes of Gordon Grau Scientific.

For further information please contact Barry Gordon Fookes at
407-931-1975.





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sun, 7 May 1995 18:33:56 -0500 (CDT)
Subject: ?Enlargers for use in EM labs

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From: smithj-at-acad.winthrop.edu
Date: Sun, 7 May 1995 13:57:58 -0400
Subject: ?Enlargers for use in EM labs

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EM folk:
We are looking to replace the enlarger in our EM facility (currently,
an Omega B66 for 35mm and a nearly-worn-out D2 for plate film). Through
past experience, I like the Durst Laborator (glass carrier, so plate
film stays flat, solidly built, 3-lens turret allows easy switching
among film sizes). The downside to the Durst seems to be the need
to switch those very expensive Latico condensors, which are easily
dropped and banged around by students. I have also been considering
a used LogEtronics automatic, as that would seem to shorten the student
learning curve in the darkroom considerably. So, my questions:
--What are you folks using, and what do you like (and not like) about it (them)
--Where does one buy used, large-format enlargers?
TIA
Julian Smith III
Winthrop University
Rock Hill, SC 29733
803-323-2111
803-323-2246 (fax)
smithj-at-winthrop.edu




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sun, 7 May 1995 18:16:26 -0500 (CDT)
Subject: Rapid freeze/freeze sub

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From: baskin-at-biosci.mbp.missouri.edu (Tobias Baskin)
Date: Thu, 4 May 1995 12:06:21 -0500
Subject: Rapid freeze/freeze sub

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Greetings,
Can formaldehyde be used as a fixative during freeze
substitution? Is powder pfa soluble in acetone? ethanol? methanol? Would
formaldehyde in acetone actually act as a fixative? My (shaky)
understanding of formaldehyde fixation in aqueous solution is that water
chemistry is crucial. I would appreciate comments or experiences about the
use of formaldehyde in freeze fix protocols.

In my system I can't use glut for reasons that I think are peculiar
to my system. In most papers I have read about freeze sub, paraformaldehyde
is not mentioned, or sometimes is just said to be inferior to glut. My
application is actually at the light level, not em, so I am willing to
accept less than the best ultrastructure, for the sake of several other
advantages that rapid freeze, freeze substitution provides. Hence my
questions about formaldehyde.

Thanks in advance,

Tobias Baskin

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
___ ____ ^ ____ _____ Tobias I. Baskin
/ \ / / \ / \ / University of Missouri
/ | / / \ / / Biological Sciences
/___ / /__ /_____\ / /__ 109 Tucker Hall
/ / / \ ( / Columbia, MO 65211 USA
/ / / \ \ / voice: 314-882-0173
/ /____ / \ \____/ /_____ fax: 314-882-0123






From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sun, 7 May 1995 18:34:45 -0500 (CDT)
Subject: computers and microscopy workshop

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From: Joyce Craig :      bafpjec-at-uxa.ecn.bgu.edu
Date: Fri, 5 May 1995 10:19:13 -0500 (CDT)
Subject: computers and microscopy workshop

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COMPUTERS AND MICROSCOPY: A free* workshop to be presented by THE MIDWEST
SOCIETY OF ELECTRON MICROSCOPISTS (MSEM) at the University of
Wisconsin-Madison, Friday and Saturday, June 2 and 3, 1995.

SCHEDULE:

June 2

1:00 Introduction: Computers in Microscopy and Microanalysis. What Can
They Do for You!!!!!! Nestor Zaluzec.
2:00 Telepresence Microscopy. Life in the Fast Lane!!!!!!! Nestor.
3:00 Break
3:30 How to Connect to the Internet. Nestor
5:00 Business Meeting
6:00 Picnic- Beer Tasting, Brats, Hamburgers and the Relishes. Cash
8:00 Informal on-line sessions: Exploring the Internet, cruising the
Internet, downloading FTP, etc.

June 3

9:00 Digitizing Negatives, Positives, Grabbing Images with Scope
Cameras. Grayson Scott
10:00 Image Processing, Adobe Photoshop, IPLab, Optimas and NIH Image.
Adobe Artist. Charles Thomas
11:00 Archiving images, Printing options. Scott Taylor, Vital Image
Technology
12:00 Tour of IMR (Integrated Microscope Resource) See two photon and latest
confocal microscopes in operation. Colleen Lavin

*Free to members. Membership is only $10 per year, $5 for students.

For reservations and further information, respond to:
Grayson Scott, University of Wisconsin (608)262-2993, FAX 608 -262-7306, E
Mail: glscott-at-facstaff.wisc.edu

See you there! Joyce Craig, Program Coordinator, MSEM (Soon to be M cubed).





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sun, 7 May 1995 18:17:48 -0500 (CDT)
Subject: Silver Paint

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From: GVKM07A-at-prodigy.com (DR CHARLES A GARBER)
Date: Thu, 04 May 1995 10:36:10 EDT
Subject: Silver Paint

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Regarding the message about silver paint from Wil Bigelow:

1] Silver paints are not all created equal.

2] "Quality" in a silver paint is more complicated than is generally
recognized, but quick drying, without the formation of a surface
barrier "skin" leaving a wet interior (which would result in excessive
out gassing in the vacuum) is one of the more important quality
considerations.

3] A fast evaporating solvent is desirable as well, but once the
volatility of the carrier exceeds a certain point the shipping charges,
especially internationally, become far greater. Also, the higher
volatility solvents are generally recognized as being more of an
inhalation hazard as well.

4] At least for the SPI #5001 (1/2 oz) and SPI #5002 (1 oz) silver
paint products, the products are formulated with a small amount of a
"special" polymer, which serves two purposes: a) it makes the paint
somewhat more "adhesive" in nature, and b) it also facilitates the
resuspension and recovery of the silver metal colloid in the event the
cap is left off the bottle and it dried out into a "brick". This does
require the use of our SPI #5004 silver paint thinner, and once some
has been added, after a few minutes in an ultrasonic, the "brick" will
completely resuspend, and the silver paint will be completely
rejuvenated. One can use other "solvents", as you have suggested, but
the degree of dispersion will not be as fine as with the #5004 thinner.
For example, there will be greater tendency to form a "skin" when the
paint dries.

We offer a free replacement bottle of SPI Silver Paint for anyone who
finds that they can not resuspend the SPI Silver Paint with #5004
thinner as indicated above. Just return the bottle that won't
resuspend so that we can find out what went wrong. PS: No one has
ever taken us up yet on this offer!

Charles A. Garber
PRESIDENT
SPI SUPPLIES
PO BOX 656
WEST CHESTER, PA 19380 USA

Ph: 1-(800)-2424-SPI [Toll free from USA]
1-(800)-668-2028 [In Canada]

FAX:1-(610)-436-5755

e-mail: GVKM07A-at-prodigy.com





From: bergrh-at-cc.memphis.edu (R. Howard Berg)
Date: Mon, 08 May 1995 09:00:45 +0600
Subject: Re: Rapid freeze/freeze sub

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Formaldehyde, even that made fresh from paraformaldehyde, exists mainly as
methylene glycol in equilibrium. Consequently, it is not an effective
fixative in fixations shorter than about 16 hours (see Fox et al. J
Histochem. Cytochem. 33:845-853 [1985]).




From: bergrh-at-cc.memphis.edu (R. Howard Berg)
Date: Mon, 08 May 1995 09:00:45 +0600
Subject: Re: Rapid freeze/freeze sub

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R. Howard Berg, Ph.D.
Biology Department
University of Memphis
Memphis, TN, 38152
E mail: bergrh-at-cc.memphis.edu
phone: 901-678-4449 fax: 901-678-4457







From: images1-at-biosci.mbp.missouri.edu (Michael Stanley)
Date: Sun, 7 May 1995 21:33:34 -0500
Subject: Porter-Blum Glass Knife-Holder

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Message-Id: {v01510101abd3332c820a-at-[128.206.15.185]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Greetings,
We have a user that is interested in cutting plastic sections on a
paraffin microtome. I have found that an old Porter-Blum glass knife
holder will fit perfectly into the paraffin holder. Anyone have, or know
of anyone else, with a holder? Or a whole Porter-Blum microtome, with a
knife-holder, that they would like to get rid of?
TIA,

C. Michael Stanley, Ph.D.
Coordinator, Associate Director
Molecular Cytology Core Facility
Molecular Biology Program
2 Tucker Hall
University of Missouri-Columbia
Columbia, MO. 65211
(314) 882-4895
fax= 314-882-0123






From: Gunnel.Karlsson-at-vf.slu.se (Gunnel Karlsson)
Date: Mon, 8 May 1995 16:45:43 +0100
Subject: Etching Lowicryls

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Message-Id: {199505081444.QAA18421-at-alnus.slu.se}
X-Sender: gunnelka-at-alnus.slu.se
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Hello,

we need to etch lowicryl (HM20) sections but we don't know if we can use
hydrogenperoxide and if so how long do we need to etch?

Gunnel







From: Gunnel.Karlsson-at-vf.slu.se (Gunnel Karlsson)
Date: Mon, 8 May 1995 16:49:41 +0100
Subject: IGSS background on supporting film

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Message-Id: {199505081448.QAA18472-at-alnus.slu.se}
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Hello,

can someone please tell us what we do wrong when we get heavy background
marking on the supporting film (formvar) but not on the sections themselfs?

Gunnel






From: _ _ _ _ _ MARK W. LUND _ _ _ _ _ :      LUNDM-at-PHYSC2.BYU.EDU
Date: Mon, 8 May 1995 09:08 MDT
Subject: Re:Absorption jump ratio

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Huang,
The jump ratio is the ratio of counts in the spectrum just above the
absorption egde to that just below the edge. In my case the
absorption edge is that of the detector (silicon, or one of the
mercuric iodide edges) and a broad band x-ray source. It is used
to estimate the thickness of the dead layer at the front of the
detector.
regards
mark

Mark W. Lund, PhD
Director
MOXTEK, Inc.
Orem UT




From: NoahHadas-at-aol.com
Date: Mon, 8 May 1995 11:22:20 -0400
Subject: Confocal Survey

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I have been asked to conduct a *very brief* survey of scientists who are
interested in, or users of, confocal microscopy...but who are NOT the
principal owners or operators of this type of instrument. The results of
this survey will be utilized by a large instrument manufacturer and your
participation will therefore have a significant impact on what type of
instruments may be available in the future.

If you would like to participate, and meet the profile mentioned above,
please respond DIRECTLY TO ME. Do not simply hit the reply button. Send your
message to: maximsci-at-aol.com

Noah Hadas




From: MacisNo1-at-aol.com
Date: Mon, 8 May 1995 11:55:07 -0400
Subject: Adobe Photoshop Help

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Hello,
I am trying to do background subtractions using Photoshop. I called
Adobe, but they keep referring me to the Magic Wand Tool. I try to explain
that I do not want to erase a red balloon from a bunch against a blue sky or
something like that. I am working with micrographs and want eliminate the out
of focus junk like dust in the optics etc. This leaves them puzzled because
they have no idea about scientific applications.

I have been trying to use the Subraction function on the Source, but the
result leaves me a grey scale image and deletes the color information. This
is a simple procedure for Hamamatsu's Argus prossessor boxes or even NIH
Image in B/W (Does Image work with color images?). In any event, could
someone lend some advice and tell me what we are doing wrong? or can Adobe
Photoshop even accomplish this? or what stand alone Image Analysis software
can do this, any platform, but preferably Macintosh. Thank you!

Lawrence Kordon
Nikon, Inc.
Baltimore, MD




From: Steven W. Miller :      73150.2217-at-compuserve.com
Date: 08 May 95 15:46:59 EDT
Subject: Photographic enlargers

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Message-Id: {m0s8ZIO-0001xsC-at-pegasus.cc.ucf.edu}

A message from Steve Miller, an authorized Durst dealer.

There really are several issues here:
1. The best enlarger for negatives with very large contrast range.
2. The highest definition enlarger.
3. The enlarger easiest to use.
4. The enlarger that survives somewhat casual users.

Without question the Durst Femopoint (point source) enlarger has the highest
definition of any enlarger I have seen. If taken to the limits you will clearly
resolve every grain of silver in the negative, as well as every scratch and
sometimes defects in the emulsion.

The Log-E (now Egoltronics) enlarger will not match this definition but is
outstanding for negatives with large contrast ranges. There are some cautions in
using a scanning spot, be careful with the illumination when the objects of
interest approach the size of your scanned spot.

The Durst Point source requires knowledge of alignment of the filament and the
rest of the optics to get the high performance. If you are not doing a critical
alignment you are not getting point source performance.

The second issue with the Durst is that the coated condenser lenses that
prevent any internal reflections can be easily abused. The coatings can be
ruined by one careless user who puts his fingers on the coating or attempts to
clean it. I recommend that student labs use a second set of condensers without
the coating (much less expensive also).

For easiest to use, just replace the point source lamp with a diffuse bulb and
all the critical aspects of alignment disappear.

I don;t wish to waste a lot of space on the net with more detail. If it is
desired please contact me off-line. We have detailed instructions on how to
align a point source enlarger.

We have represented LogE in the past, and are Durst and Omega dealers now.

Steven W. Miller
Integrated Microsystems, Inc.
Phone 800-388-8801
Fax 708-696-2541
email 73150.2217-at-compuserve.com





From: Michael Shaffer :      mshaf-at-darkwing.uoregon.edu
Date: Mon, 8 May 1995 15:01:02 +0000
Subject: EDX computer for sale

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Message-Id: {199505082154.OAA13468-at-darkwing.uoregon.edu}
Comments: Authenticated sender is {mshaf-at-darkwing.uoregon.edu}

We are considering upgrading our 1.5 year old Oxford Link eXL to
Oxford's ISIS system. I want to be brief here, 'cept to mention the
system is "loaded" and that it will be sold without the detector and
the stage motors.
If any SEM/EMPA facility is interested in knowing more about the eXL
please reply to me directly (email or phone).

{\/} /\ {\/} /\ {\/} /\ {\/} cognito, ergo zZOoooOM {\/} /\ {\/} /\ {\/} /\ {\/}

http://darkwing.uoregon.edu/~mshaf/epmahome/

Michael Shaffer, R.A. mshaf-at-oregon.uoregon.edu (days)
Electron Probe Facility mshaf-at-darkwing.uoregon.edu (any time)
Geological Sciences
1272 University of Oregon (503)346-4632
Eugene, OR 97403-1272 (503)346-4692 (FAX)




From: Dwight Beebe :      beebed-at-ERE.UMontreal.CA
Date: Mon, 8 May 1995 18:05:38 -0400 (EDT)
Subject: NIH-Image group address?

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Hi,
I understand that there is a group similar to this one for
questions, etc. regarding Image. Would someone be kind enough to point
me in the right direction? We're starting to use Image to work with
scanned autoradiographs of C14-labelled leaf material and need some hints.
Many thanks and please respond to me directly to skip sending tangential
info like this to all subscribers.

Dwight Beebe
IRBV, Dept. de sciences biologiques beebed-at-ere.umontreal.ca
Universite de Montreal Voice:514-872-4563
4101, rue Sherbrooke est FAX:514-872-9406
Montreal, PQ H1X 2B2 Canada







From: Lata Prabhu 512-356-7894 :      Lata.Prabhu-at-SEMATECH.Org
Date: Mon, 08 May 1995 16:03:00 -0500 (CDT)
Subject: RE: ?Enlargers for use in EM labs

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MR-Received: by mta MAILV2; Relayed; Mon, 08 May 1995 16:17:41 -0500 (CDT)
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Hop-count: 2


Julian:
We are currently using an enlarger manufactured by Charles Bessler.
It is Bessler Model#45MXT. which uses 150w bulb for its condenser
light source and so far in the past two years I have worked for
Sematech, I have barely changed it once.There are different sizes
available for negative film holders.In our lab we mostly use SO163
and the holder is model#8390.Although the exposure is not
automatic, and we always have to use a test strip for exposure for
every negative,I think it is very friendly and will be ideal for
student use.We doquite a lot of high resolution TEM photography and
get good quality enlargements.
It is a table top mounted and we use the standard photographic
easel designed to hold 5x7, 8x10 and 10x13 papers.
If you want I can find more information about it and the price
range,since this was here before my arrival here at Sematech.
Good luck with your search,
Lata





From: kayton-at-ohsu.edu (Robert Kayton,MAC,CROET)
Date: Mon May 8 12:15:01 PDT 1995
Subject: Pacific Northwest EM Society Meeting

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Message-Id: {m0s8YGk-0007BzC-at-stjohns.ohsu.edu}
Message-Version: 2
} To: Microscopy-at-aaem.amc.anl.gov


PACIFIC NORTHWEST ELECTRON MICROSCOPY SOCIETY
1994 SPRING MEETING
"ELECTRON MICROSCOPY PREPARATION"
Veterans Administration Medical Center
3710 SW Veterans Hospital Road
Portland OR 97201
AND
Oregon Health Sciences University
3181 SW Sam Jackson Park Road
Portland OR 97201

Friday, May 12- Veterans Administration Medical Center Auditorium


9:30 a.m. "Cytoskeleton of the Wistar-Furth rat's Megakaryocytes and
Platelets"
Paula Steinberg Pathology, Oregon Health Sciences
University, Portland, OR.
10:00 a.m. "Ultrastructure of Connective tissue using Cryotechnical
Processing."
Doug Keene, Shriners Hospital, Portland, OR.
10:30 a.m. "Advantages of using FIB Techniques to Prepare Samples"
Dave Laken, FEI Company, Beaverton, OR.
11:00 a.m. Break Coffee
11:15 a.m. "Low Vacuum SEM"
John Grimes, JEOL USA, Inc., Palo Alto, CA.
12:00 p.m. PNEMS Business Meeting
3:00 p.m. "Special Immunogold Techniques"
Charlie Meshul, Veterans Administration Medical Center,
Portland,OR.
3:30 p.m. "X-Ray Microscopy: Seeing Things in a New Light"
Chris Jacobsen, SUNY Stony Brook, Stony Brook, NY.
4:30 p.m. Adjourn
4:45 p.m. Social
Atrium of the Center for Research on Occupational and
Environmental Toxicology. 3rd floor. Oregon Health Sciences
University.
Saturday, May 13th-Center for Research on Occupational and Environmental
Toxicology. Room 2564.
10:00 a.m. "Cryosectioning Materials: A Workshop"
Greg Becker, Research and Manufacturing Company, Tucson AZ
12:00 p.m. Lunch
1:30 p.m. "Practical application of Cryosectioning Materials"
Greg Becker, Research and Manufacturing Company, Tucson AZ
4:00 p.m. Adjourn

Bob Kayton, PNEMS
Oregon Health Sciences University
C.R.O.E.T., L606
Portland, OR 97201
(503)494-2504
(503)494-6831 Fax
e-mail kayton-at-ohsu.edu




From: Luc Analbers :      L.J.S.Analbers-at-med.ruu.nl
Date: Tue, 09 May 1995 10:02:50 +0200
Subject: intracellular [Ca2+]

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Dear confocal members,

We would like to have a reply on 2 questions. Maybe you can help us.

1. Does anyone have measured, or knows a reference of intracellular [Ca2+] in
quiesent heart cells, preferably from neonatal rats?

2. Does anyone know software to calculate free [Ca2+] in solutions.


Thanks.

Luc Analbers
University Utrecht
The Netherlands

Analbers-at-med.ruu.nl




From: Software department :      software-at-oimag.win-uk.net
Date: Tue, 09 May 1995 08:57:29
Subject: Re:Absorption jump ratio

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X-Mailer: WinNET Mail, v2.30
Message-ID: {629-at-oimag.win-uk.net}
Reply-To: Software department {software-at-oimag.win-uk.net}
To: microscopy-at-aaem.amc.anl.gov

Sorry to be picky....

I think Mark is talking about a symptom of the absorption jump
ratio - I always thought the absorption jump ratio was simply the
ratio of mass absorption coefficients on either side of the
absorption edge - since the absorption coefficient goes through a
discontinuity and "jumps" at the point of the edge - the
absorption jump ratio is simply what it says.

The step in the continuum at the absorption edge is caused by
differential absorption - for a thickness x, the transmitted beam
intensity is exp(- mu.ro.x). mu is the mass absorption
coefficient, ro is the density. If you look at absorption pre and
post edge, the ratio of absorptions will be exp(- dmu. ro. x)
where dmu is the change in mu across the edge. The size of the
step clearly depends on the absorbing path.

Peter Statham

} Huang,
} The jump ratio is the ratio of counts in the spectrum just above the
} absorption egde to that just below the edge. In my case the
} absorption edge is that of the detector (silicon, or one of the
} mercuric iodide edges) and a broad band x-ray source. It is used
} to estimate the thickness of the dead layer at the front of the
} detector.
} regards
} mark
}
} Mark W. Lund, PhD
} Director
} MOXTEK, Inc.
} Orem UT
}

-----------------------------------------------------------------
Please reply to this e-mail with the name of the person you
wish to receive it on the subject line
(e.g. "FAO Jane Smith/..subject.."),
as this is a shared e-mail address.






From: randio-at-fagmed.uit.no (Randi Olsen)
Date: Tue, 09 May 1995 14:32:07
Subject: Muscle tissue.

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Hello everyone!
Two studenst asked me to pass this message on to you:

We (Ann Kristin Lorentzen and Kathleen Jacobs) are two last year bioengeneer
students at Tromsoe College in Norway, who are working on a project in
transmission electron microscopy.
We would appreciate any information about the epoxy resins Epon/Araldite,
Spurr and Taab 18's infiltration ability into muscle tissue, and eventual
differences of quality.
(The Department of Pathology are not quite satisfied with muscle biopsies
embedded in Epon/Araldit, and the problems are probably related to poor
infiltration)

Thank you for your help.

Sincerely,
Ann Kristin Lorentzen and Kathleen Jacobs





From: randio-at-fagmed.uit.no (Randi Olsen)
Date: Tue, 09 May 1995 14:45:07
Subject: Muscle tissue.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello everyone!
Two students asked me to pass this message on to you:

We (ann Kristin Lorentzen and Kathleen Jacobs) are two last year bioengeneer
students at Tromsoe College in Norway, who are working on a project in
transmission electron mictoscopy.
We would appreciate any information about the epoxy resins Epon/Araldite,
Spurr and Taab 18's infiltration ability into muscle tissue, and eventual
differences of quality.
(The Department of Pathology are not quite satisfied with their muscle
biopsies embedded in Epon/Araldit, and the problems are prabably related to
poor infiltration).

Thank you for your help.

Sincerely,
Ann Kristin Lorentsen and Kathleen Jacobs

----------------------------------------------------------------y
Randi Olsen
University of Tromsoe
Department of Electron Microscopy
Institut of Medical Biology
N9037 Tromsoe

e-mail {randio-at-fagmed.uit.no}





From: Alan S. Pooley :      pooley-at-ahab.rutgers.edu
Date: Tue, 9 May 1995 09:20:34 -0400 (EDT)
Subject: Re: Help with EMPA of Minerals

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I am not sure if you mean located on the internet or willing to be listed
there but physically located at some lab, I am assuming the latter:
I have 2 sem's and 2 image analysis systems, one is pc based, indepdant
from the sem's, the second image system is RT11 (DEC) based EDAX system
attached to the amray 1830I sem. The ETEC sem currently has no system
but we hope to put a 4pi analysis system on it. I could go on but
perhaps you could send me a form or indicate how you want and if you
want more info. All equipement here is available to users, for a fee,
with or without expert assistance. I have handled all kinds of sem & eds and
would be wulling to consult or work with any form of sample (well also
any).

Alan S. Pooley ,PhD Bivalve shell SEM & shape analysis
SEM/Morphometrics lab
Marine & Coastal Sciences
Rutgers University
908 932 8959 ext 225
Pooley-at-ahab.rutgers.edu

On Thu, 4 May 1995 MICROSCOPY-at-AAEM.AMC.ANL.GOV wrote:

} From: SMTP%"Spignolet-at-aol.com" 3-MAY-1995 23:32:02.24
} To: MICROSCOPY
} CC:
} Subj: Help with EMPA of Minerals
}
} Date: Mon, 1 May 1995 22:44:28 -0400
} From: Spignolet-at-aol.com
} Message-Id: {950501215443_103354647-at-aol.com}
} To: Microscopy-at-aaem.amc.anl.gov
} Subject: Help with EMPA of Minerals
}
} This message below was posted to sci.res. I have sent him JEOL and Links
} addresses. If anyone has info on Bence Albee or other information handy
} could you please send it directly to him.
}
} Also, I am collecting infomation on microscopy and image analysis resources
} on the Internet. The information will be available at a web site for
} everyone and on disk and hard copy at EMSA (for a small fee that will cover
} costs and FREE to those that contribute).
}
} Thanks
}
} Susanne Pignolet-Brandom, Ph.D.
} MicroWorld Resources and News
} 708-548-6522
}
}
} From: sysop-at-pfzrfsg.com (Michael Wallach)
}
} }
} } I am trying to get information about the equipment listed below. The
} } address/phone number of the manufacturer would be appreciated. I am also
} interested in any literature references regarding its use for the analysis of
} minerals.
} }
} } Quantitave Electron Probe Micro-Analysis (QEPMA) consisting of:
} } JEOL JXA 8600 Superprobe
} } Link Analytical AN 10/85S X-ray analysis system
} }
} } --- Via Silver Xpress V4.02B03 PFI-12521
} }
} } -----------------------------------------------------------------------
} ------
} } Email: sysop-at-pfzrfsg.com (Michael Wallach)
} } Pfizer Food Science Group, New York
} } -----------------------------------------------------------------------
}




From: Joyce Craig :      bafpjec-at-uxa.ecn.bgu.edu
Date: Tue, 9 May 1995 09:00:06 -0500 (CDT)
Subject: Re: Muscle tissue.

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On Tue, 9 May 1995, Randi Olsen wrote:

} Hello everyone!
} Two students asked me to pass this message on to you:
}
} We (ann Kristin Lorentzen and Kathleen Jacobs) are two last year bioengeneer
} students at Tromsoe College in Norway, who are working on a project in
} transmission electron mictoscopy.
} We would appreciate any information about the epoxy resins Epon/Araldite,
} Spurr and Taab 18's infiltration ability into muscle tissue, and eventual
} differences of quality.
} (The Department of Pathology are not quite satisfied with their muscle
} biopsies embedded in Epon/Araldit, and the problems are prabably related to
} poor infiltration).
}
} Thank you for your help.
}
} Sincerely,
} Ann Kristin Lorentsen and Kathleen Jacobs
}
} ----------------------------------------------------------------y
} Randi Olsen
} University of Tromsoe
} Department of Electron Microscopy
} Institut of Medical Biology
} N9037 Tromsoe
}
} e-mail {randio-at-fagmed.uit.no}
}
of course the department of pathology is dissatisfied - Let me guess -
They are giving you huge pieces of muscle, right? I had the same problem
many years ago and asked for help as you are.
I have found that overnight in 1:2 propylene oxide:epoxy helps, if you
must infiltrate pieces of muscle thicker than 1mm. Extend all times on
all steps, which will help. Use freshly mixed resin for the all resin
infiltration.
Where is TROMSOE?}




From: DIRK DOMASCHKO :      DOMASCHK-at-musom01.mu.wvnet.edu
Date: Tue, 09 May 1995 10:50:14 +1100
Subject: Re: Muscle tissue.

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Tuesday May 9

} Hello everyone!
} Two studenst asked me to pass this message on to you:
}
} We (Ann Kristin Lorentzen and Kathleen Jacobs) are two last year bioengeneer
} students at Tromsoe College in Norway, who are working on a project in
} transmission electron microscopy.
} We would appreciate any information about the epoxy resins Epon/Araldite,
} Spurr and Taab 18's infiltration ability into muscle tissue, and eventual
} differences of quality.
} (The Department of Pathology are not quite satisfied with muscle biopsies
} embedded in Epon/Araldit, and the problems are probably related to poor
} infiltration)
}
} Thank you for your help.
}
} Sincerely,
} Ann Kristin Lorentzen and Kathleen Jacobs
}
}

Spurr's and Spurtol are great for muscle tissue. I have used
both of these to embed my rat muscle tissue for TEM and never been
dissatisfied with the results.
By the way Spurtol and Spurr's (firm) embeded muscle tissue slice
very nicely.

Any recipe info you might like I would be happy to send you.







Dirk W. Domaschko
Electron Microscopy Facility
Marshall University School of Medicine
Huntington, WV 25755-9350
Ph: 304-696-7393
E-Mail: Domaschk-at-musom01.mu.wvnet.edu




From: GANTZ-at-med-biophd.bu.edu
Date: Tue, 09 May 1995 12:04:13 -0500 (EST)
Subject: ENLARGERS

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We've been using Omega enlargers for a number of years with complete
satisfaction. Four years ago we purchased a used D2V from a firm in New
Jersey specializing in used equipment. At that time, they were offering
TEM's, SEM's, and a host of preparative equipment and accessories. Don't
know if firm still exists but here is info:
Microscopy Laboratories
P.O. Box 338
61 West Street
Red Bank, N.J. 07701
908-747-6228
Good Luck, Don




From: emlab-at-ucsco.ucsc.edu (Jon Krupp)
Date: Tue, 9 May 1995 09:26:04 -0700
Subject: Diamond Cell Sample Prep?

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Message-Id: {199505091625.JAA10574-at-ucsco.ucsc.edu}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

This is a two part question about preparing samples from diamond pressure
cells. The first part is a question about measuring the thickness of the
sample, the second question is about preparing the sample for TEM.

Several mineral physics types on my campus use diamond cells to investigate
the changes in rocks that take place at high temperatures and pressures. I
am not a mineral physics type, or even a geologist, but I am the campus EM
person and I need to try to help them. A diamond cell is a device, 30 mm
square and about as thick, that holds small samples, less than 1 mm,
between two diamond faces. The sides of the holder can be compressed to
apply pressure and a laser can be sent through the diamonds to heat the
sample. The sample resides in the center of the holder and can be seen
through the diamonds, but to see it with a compound microscope requires
long working distance objectives because of the configuration of the metal
holder around the sample.

We can measure the area of the sample fairly easily, it transmits light and
we have a 20x objective that works for this. The problem is measuring the
thickness to get an idea of the volume of the sample. The sample must stay
in the diamond cell and be measured at different stages of the experiment.
I thought of trying to focus through the sample and noting the z-distance
but our objective has too great a depth of field and the sample is too
thin, 25 - 30 mu, to let this work well. I have tried using other
objectives that have less depth of field, but they have too short a working
distance and are too big to get into the space available in the diamond
cell.

Does anyone have an idea about how to measure the thickness of the sample
while it is in the cell? We are also looking for good ideas about how to
measure the thickness of the sample after it is removed from the cell. We
have tried mounting small pieces for SEM and tipping them up for a cross
section view, but we are ready to try something better.

Now for the second question, how to prepare these samples for TEM. I have
thought about microtoming and tripod polishers but don't know which one
might be the best to try first. For some reason I lean toward tripod
polishing but I don't have much to base that on. Could anyone offer
suggestions on this technique, including the equipment and approximate
costs involved in getting set up?

Thanks for your help.

Jonathan Krupp
Microscopy and Imaging Lab
University of California
Santa Cruz, CA 95064
(408) 459-2477
emlab-at-ucsco.ucsc.edu






From: MICROSCOPY-at-aaem.amc.anl.gov
Date: Thu, 4 May 1995 7:12:42 -0500 (CDT)
Subject: Help with EMPA of Minerals

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This is a MIME-encapsulated message

--LAD13065.800037605/dns2.anl.gov

The original message was received at Thu, 4 May 1995 11:29:47 -0500
from aaem.amc.anl.gov [146.139.72.3]

----- The following addresses had delivery problems -----
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From: sysop-at-pfzrfsg.com (Michael Wallach)
Date: Mon, 1 May 1995 22:44:28 -0400
Subject: Help with EMPA of Minerals

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This message below was posted to sci.res. I have sent him JEOL and Links
addresses. If anyone has info on Bence Albee or other information handy
could you please send it directly to him.

Also, I am collecting infomation on microscopy and image analysis resources
on the Internet. The information will be available at a web site for
everyone and on disk and hard copy at EMSA (for a small fee that will cover
costs and FREE to those that contribute).

Thanks

Susanne Pignolet-Brandom, Ph.D.
MicroWorld Resources and News
708-548-6522



}
} I am trying to get information about the equipment listed below. The
} address/phone number of the manufacturer would be appreciated. I am also
interested in any literature references regarding its use for the analysis of
minerals.
}
} Quantitave Electron Probe Micro-Analysis (QEPMA) consisting of:
} JEOL JXA 8600 Superprobe
} Link Analytical AN 10/85S X-ray analysis system
}
} --- Via Silver Xpress V4.02B03 PFI-12521
}
} -----------------------------------------------------------------------
------
} Email: sysop-at-pfzrfsg.com (Michael Wallach)
} Pfizer Food Science Group, New York
} -----------------------------------------------------------------------

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From: slc6-at-Lehigh.EDU (Sharon Coe)
Date: Tue, 9 May 1995 14:28:08 -0400
Subject: Post-doc position at Lehigh University

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POST-DOCTORAL RESEARCH ASSOCIATE
Department of Materials Science and Engineering
Lehigh University

Lehigh University Department of Materials Science and Engineering is
seeking a post-doctoral research associate to work on extended
electron-energy-loss fine structure studies of glasses using VG HB 603 &
501 instruments, both equipped with Gatan PEELS. The successful candidate
must have a doctorate with a strong background in electron spectroscopy in
the TEM/AEM. Experience with Gatan PEELS and Vacuum Generators microscopes
and fine structure analysis (EXELFS or ELNES) is highly desirable. The
position is open from August 1995. Initial appointment for one year, with
extension to three years depending on performance. Lehigh University is an
Equal Opportunity, Affirmative Action employer.

Please send resume to:

Dr. David Williams
Department of Materials Science & Engineering
Lehigh University
5 East Packer Avenue
Bethlehem, PA 18015






From: South Bay Technology :      73531.1344-at-compuserve.com
Date: 09 May 95 15:50:25 EDT
Subject: Diamond Cell Sample Prep?

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Jonathan-

I can't really help too much on the first question of measuring your samples
while in the diamond cell, but I can answer part of your question about Tripod
Polishing.

We have been manufacturing the Tripod Polisher for over 6 years and I must say
that I am constantly amazed at the variety of samples that people have been able
to prepare. I can probably give you some direction on the technique, but I'd
like to learn a bit more about your specific sample. For example:

1) Actual Size of samples
2) Material
3) Is there a specific area you need to cross-section or will anywhere on the
sample suffice?
4) Is the sample sensitive to water or other materials?
5) Can the sample be heated?

Basically, anyhting you can tell me about the sample would be helpful.. I'll
send you a copy of a paper on Tripod Polishing that should help a bit - although
it obviously won't deal with your particular sample.

Regarding cost, that can vary a bit depending on your answers to the above
questions. In general I would recommend our Model 590W Tripod Polisher for TEM
Wedge Polishing. This system will give you everything you will need as far as
the fixturing goes and it sells for $875. Yo willalso need to invest in some
diamond lapping films, colloidal silica, some polsihing cloths, a glass plate
and a few other items. If you don't already have these items, it'll proabbly
cost you a few hundred bucks to get an initial supply. If you'd like, I'll be
glad to send you a complete price list that will tell you exactly how much it
will cost.

The other thing you'll need is a high quality polishing wheel. While variable
speed metallurgical wheels are adequate, it is best to have a polisher that is
capable of running at very constant low rpm and with a fair amount of torque.
Of course, anything will do in a pinch, but if you have the money and the
desire, we have a machine which is designed for Tripod Polishing that runs
$3475.00. It is also helpful to have an inverted stage metallograph, but you
can also make the system work with a decent stereo microscope.

I hope this information helps! Please feel free to contact me if you have any
other questions.

Best regards-

David Henriks TEL: 800-728-2233 (toll-free in USA)
South Bay Technology, Inc. 714-492-2600
1120 Via Callejon FAX: 714-492-1499
San Clemente, CA 92673 USA e-mail: 73531.1344-at-compuserve.com





From: Glenn Poirier :      GLENN_P-at-GEOSCI.Lan.McGill.CA
Date: Tue, 9 May 1995 16:28:47 EST5EDT
Subject: used optical microscopes

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Message-Id: {199505092029.QAA12316-at-sifon.CC.McGill.CA}

Hi,
A few months (years?) ago I remember seeing a reference to a company
that dealt in second hand optical microscopes. Now that I am looking
for a second hand scope I can't seem to find the message. Could
anyone help me with this?

Thanks

Glenn
**********************************************************************
* Glenn Poirier glenn_p-at-geosci.lan.mcgill.ca *
* Electron Microprobe Lab Phone: (514) 398 6774 *
* Earth and Planetary Sciences Fax: (514) 398 4680 *
* McGill University THERE ARE THREE SIDES TO EVERY STORY; *
* Montreal, Quebec YOUR SIDE MY SIDE AND THE TRUTH *
**********************************************************************




From: Daniel Henne :      henne-at-sfu.ca
Date: Tue, 9 May 1995 15:51:04 -0700 (PDT)
Subject: Looking for article

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Hello I'm in a bit of a bind. None of the libraries I have access to
(Simon Fraser University and Univ. of British Columbia) are carrying
Scanning. The article is:
G.Love and V.D.Scott, Scanning, 1981, 4, 111.
I believe that is vol. 4 page 111. If anyone would be willing to Fax
this to me I would greatly appreciate it. Please email me so I can give
you my fax number.

Thank you in advance
Dan Henne
Simon Fraser University
Vancouver, Canada
email:henne-at-sfu.ca





From: bozzola-at-siu.edu (John. J. Bozzola)
Date: Tue, 9 May 1995 18:31:03 -0600
Subject: Voyager III Directions

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X-Sender: bozzola-at-saluki-mail.fiber2.siu.edu
Message-Id: {v01510102abd5b8a6b64c-at-[131.230.97.61]}
Mime-Version: 1.0
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Hello Microscopists:

We have a Noran Voyager III and are looking for some user-generated sets of
directions, tips, shortcuts, schedules, etc. Anyone have any good sets they
would be willing to share with us? By the way, we have many directions for
various lab instrumentation that we routinely share as well - if you are
interested. Thanks.

John J. Bozzola
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
Phone: 618-453-3730
Fax: -2665
Email: bozzola-at-siu.edu OR bozzola-at-qm.c-cem.siu.edu






From: randio-at-fagmed.uit.no (Randi Olsen)
Date: Wed, 10 May 1995 14:25:22
Subject: Immunolabelling of osteoclasts.

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Message-Id: {199505100756.DAA15832-at-interramp.com}

Hello again, out there.

We are going to start a project which involve immumolabelling of osteoclasts.
These are cells we are not familiar working with, and we would appreciate
any ideas how to "find" them (bone-marrow aspirate, "whole" bone-marrow or
isolation).
I would also like your opinion about sectioning techniqe, cryo-sectioning or
embedding in Lowicryl or other suitable resins of these cells/tissue.

Thanks!
--------------------------------------------------------------------
Randi Olsen
University of Tromsoe
Department of Electron microscopy
MH-Breivika
N-9037 Tromoe
Norway
--------------------------------------------------------------------------





From: randio-at-fagmed.uit.no (Randi Olsen)
Date: Wed, 10 May 1995 15:23:20
Subject: Where is Tromsoe?

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Hello,
Thanks for the response on the question about miscle tissue.
Joyse Craig added another: Where is Tromsoe? which give me an oportunity to
tell you that Tromsoe is situated in the north of Norway (some say 'next to
the North Pole'), 400-500 km north of the Polar Circle.
The only reason it's possible to live here and grow stawberries in the
summer (apples don't go) are warm water from the Mexican gulf, the Gulf
Stream. That give us temperatures never below -20 C in the winter.
Beside very nice nature around the town, we also have midnight sun 2 months
in the summer (and two dark months without any sun in the winter, but then
we often have a very nice Aurora Borealis 'Nothern light').There are now 2
weeks until we have sun during the night too, and it's only 15 cm of snow
where I live.

Tromsoe is a small town with apr. 50 000 inhabitants, mostly norwegians of
cause, but we have the nothermost univeristy of the world, (6 600 students)
and that has brought students and scientists from all over the world to the
University.
The EM facility here belongs to the university and are used by scientists
from all over the campus (geology, biology, medicin etc) and the hospital
has 2 bioengeneers working here. The department are very well equiped and we
have nice facilities in a 3 years old building.

Like to visit us?

Best wishes
Randi

--------------------------------------------------------------------
Randi Olsen
Univeristy of Tromsoe
Department of Electron Microscopy
N-9037 Tromsoe
Norway





From: William R. Oliver :      oliver-at-ipas.afip.mil
Date: Wed, 10 May 1995 09:37:09 -0500 (EST)
Subject: Re: Photo-Subtraction

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On Tue, 9 May 1995, Azriel Gorski wrote:

} }
} } Hello,
} } I am trying to do background subtractions using Photoshop. I called
} } Adobe, but they keep referring me to the Magic Wand Tool. I try to explain
} } that I do not want to erase a red balloon from a bunch against a blue sky or
} } something like that. I am working with micrographs and want eliminate the out
} } of focus junk like dust in the optics etc. This leaves them puzzled because
} } they have no idea about scientific applications.
} }
} Sorry, this is not exactly on point, but as scientist and a Forensic Scientist
} I find that this elimination of the "out of focus junk" bothers me. It may not
} look nice, but it was in the field of view. Besides accuracy, there have been
} cases where I thought that something was "junk" and it was not.
}

Really? Do you mean that your optical microscopy lab doesn't do
background subtraction or light/darkfield correction? This doesn't
constitute decreasing accuracy; it has been a standard part of correcting
for nonstationary illumination and dust in the optics for years in
clinical quantitative microscopy. In classical background subtraction,
you use an image taken using either nothing or a blank slide. That way
you *know* that the objects present in the image are artifacts. The
danger of *not* performing these kinds of corrections is that folk will,
in fact, perceive these artifacts as features present in the specimen.
They *are* junk, and correcting for them is as much a part of adequate
quantitative microscopy as is using correct focus(1).

I also missed the first post, but I will add that Photoshop is probably
not a good way to do background subtraction. Background subtraction is,
by definition, an image algebra routine, and one should either implement
it directly or use a package that does image algebra or background
subtraction directly. Also, be careful about using any of the "artistic"
packages, since they are more concerned with aesthetic and ergonomic
questions than accuracy. For instance, Photostyler will do some image
algebra, but handles overflows very badly (for instance, if you can
represent a pixel as an integer between 0 and 255, what happens when you
add two pixels of value 200 together?).

There are a number of public domain packages which will do these sorts of
things, including Khoros, DIAL, NIH Image, and others for all sorts of
platforms. Check out the comp.sys.graphics FAQ. There are also a bunch
of commercial packages, such as Optimas, ImagePro, and Visilog, which
provide better handling than the artistic packages.

billo

1) Ken Castleman, "Digital Image Processing," 1979, p 105.




From: Ian Hall :      hall-at-me.udel.edu
Date: Wed, 10 May 1995 10:24:56 -0400 (EDT)
Subject: Tripod polishers/suppliers

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I need a tripod polisher and would appreciate receiving names
addresses, etc. of suppliers; I believe that there are at least three
companies that make them.
Also any comments about advantages/disadvantages of specific ones
would be most welcome, as would advice from the sages of the field. The
intended application is brittle intermetallic overlayers on a ductile
substrate.
With thanks

Rick Hall
Materials Science
University of Delaware
hall-at-me.udel.edu







From: jeanne_barker-at-merck.com (Jeanne Barker)
Date: 10 May 1995 10:24:47 U
Subject: Cryosectioning WAT

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Message-Id: {9505101430.AA26781-at-igw.merck.com}

I Have a Question regarding... Cryosectioning WAT
5/10/95 9:32 AM
Does anyone have any suggestions/experience in ultracryosectioning white
adipose tissue? I have tried different temperatures, and still my tissue
shatters. I am not sure what else I can vary. They are nakane fixed, and
cryoprotected with PVP.
Thanks, Jeanne






From: tvoiles-at-unlinfo.unl.edu (Todd Voiles)
Date: Wed, 10 May 1995 11:00:14 -0400
Subject: Virtual SEM Software?

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Hi,

I was wondering how I could get ahold of the CD-Rom version
of the Virtual SEM program I saw mentioned earlier on this list.

Thanks
Center for Materials Research and Analysis
Central Facility for Electron Microscopy
University of Nebraska at Lincoln

Todd Voiles
tvoiles-at-unlinfo.unl.edu





From: South Bay Technology :      73531.1344-at-compuserve.com
Date: 10 May 95 11:52:30 EDT
Subject: Tripod polishers/suppliers

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Ian-

I am only aware of 2 suppliers of the Tripod Polisher:

1) South Bay Technology, Inc. (800) 728-2233 (that's us!)
2) Allied High Tech (310) 635-2466

Obviously, I can't give you an unbiased opinion. I would suggest that you talk
to both of us and decide for yourself who is offering the better system. The
technology of building the tool itself is not exactly rocket science, but there
are a number of nuances that are only picked up through experience.

I would be curious to hear if you do come up with a 3rd company making the
Tripod Polisher. I do try to keep up on the competition, but I don't,
unfortunately, know everything! Pleae let me know if you'd like me to send any
additional information on our Tripod Polishing Systems.

Best regards-

David Henriks TEL: 800-728-2233 (toll-free in USA)
South Bay Technology, Inc. 714-492-2600
1120 Via Callejon FAX: 714-492-1499
San Clemente, CA 92673 USA e-mail: 73531.1344-at-compuserve.com





From: Michael OKeefe :      Michael_OKeefe-at-macmail7.lbl.gov
Date: 9 May 1995 16:52:20 U
Subject: NCEM Summer School

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Message-Id: {n1412083961.20861-at-macmail7.lbl.gov}
"Crispin Hetherington" {CJDH100-at-phx.cam.ac.uk} ,
"David Loretto" {loretto-at-csa2.lbl.gov} ,
"Dennis Maher" {maher-at-mat.mte.ncsu.edu} ,
"Velimir Radmilovic" {vrmimo-at-elab.tmf.bg.ac.yu} ,
"Owen Saxton" {wos1-at-cus.cam.ac.uk} ,
"Gareth Thomas" {garth-at-uclink2.berkeley.edu} ,
"Zara Weng-" {ZWeng-at-zazen.lbl.gov} ,
"Gretchen Hermes" {Gretchen_Hermes-at-macmail7.lbl.gov} ,
"Roar Kilaas" {Roar_Kilaas-at-macmail7.lbl.gov}
X-Mailer: Mail*Link SMTP/QM 3.0.0

Subject: Time:9:43 AM
OFFICE MEMO NCEM Summer School Date:5/9/95

There are still a few places left for the National Center for Electron
Microscopy Summer School on Computer Simulation and Processing
of HRTEM Images. The school is held every year at the end of June -- this
year's is June 26 - 30. For further information see --

http://ncem.lbl.gov/NCEM/workshops.html

or contact --

Gretchen Hermes
Lawrence Berkeley Laboratory
MailStop 72
1 Cyclotron Road
Berkeley, CA 94720
Phone: +1-510-486-5006
Fax: +1-510-486-5888
e-mail: GHermes-at-lbl.gov





From: jph-at-solo.com (Jean-Pierre Hebert)
Date: Wed, 10 May 1995 11:30:39 -0700
Subject: unsubscribe

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From: ard-at-atom.ansto.gov.au (Arthur Day)
Date: Thu, 11 May 1995 04:30:17 +1000
Subject: Re: Virtual SEM Software?

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Message-Id: {abd6b64205021004e893-at-[137.157.95.82]}
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Contact one of the authors,

Brendan Griffin at,

bjg-at-uniwa.uwa.edu.au


Arthur Day, Electron Microscopy Group
Ansto Advanced Materials Program Phone: 61-2-717-3457
PMB 1, Menai (Sydney), NSW, 2234 Fax: 61-2-543-7179
Australia
Email: ard-at-atom.ansto.gov.au







From: modum-at-gatan.COM (Michael Odum)
Date: Wed, 10 May 1995 11:28:45 -0700
Subject: Re: Photo-Subtraction

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Message-Id: {199505101727.KAA00111-at-core.gatan.com}

Hello,

My company developed a scientific imaging software called
DigitalMicrographT. It is capable of backround subtraction and can handle
color images. It is also made for Macintosh so you can just plug it in and
go. The person you would want to talk to about the software's precise
capabilities is Chris Meyer[cmeyer-at-gatan.com] or you can ask for him at the
below numbers. Just to share.

Sincerly,

Michael Odum
Spec. Prep. Tech.
Gatan, Inc.
6678 Owens Dr.
Pleasanton, CA 94588
Tel: 510-463-0200
Fax: 510-463-0204
E-Mail: modum-at-gatan.com





From: (colleen ann lavin) :      lavinca-at-vms2.macc.wisc.edu
Date: Wed, 10 May 1995 14:24:06 -0600
Subject: sectioning glass

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Message-Id: {25051014195071-at-vms2.macc.wisc.edu}

I would appreciate advice concerning sectioning glass fibers (average
diameter of 1 micron). The purpose of the project is to localize cells that
are filtering through the glass. I am considering using an old diamond
knife, presuming the damage may be great. I do not anticipate sectioning
this material again after the completion of this project; I would prefer
not to purchase a material science diamond knife unless it is the only tool
that would section the glass fibers.

My second concern is whether or not to embed in plastic. I have been told
embedding would require a primer (Dow Chemical product..) to help embed the
glass and then I would have to get a very hard plastic to closely
approximate the fibers themselves.Would quick freezing followed by
cryosectioning be a more logical approach? Can I use a std biological
diamond knife for cryosectioning? The resin used to glue the knife may be
weakened, but I am already considering the knife would be useless after the
project anyway.

An alternate approach would be to see if the fibers can be simply fractured
and then viewed in the SEM. Would fracturing the frozen fibers just result
in shattered glass?

Thank you very much.

Colleen Lavin


--------------------------------------} }

Colleen A. Lavin
Integrated Microscopy Resource
High Pressure Freezer Coordinator
Madison, WI 53706
608-263-8481
lavinca-at-macc.wisc.edu





From: Joseph P. Neilly 708-938-5024 :      NEILLY.JOSEPH-at-igate.abbott.com
Date: Wed, 10 May 1995 11:49:00 -0600 (CST)
Subject: Converting to LaB6

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Mr-Received: by mta RANDB; Relayed; Wed, 10 May 1995 12:13:34 -0600
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Mr-Received: by mta RANDD; Relayed; Wed, 10 May 1995 12:13:48 -0600
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Content-Return: prohibited

We are planning to convert the tungsten filament in our Philips CM-12
STEM to a LaB6 source. I am aware of the general precautions one must
take with regards to vacuum and saturation to preserve the LaB6 crystal
but not the details. I would like to know what specific precautions CM-
12 users take when using their instruments with a LaB6 filament. Is the
built in saturation delay of the instrument slow enough? What vacuum is
low enough to begin saturation? How do you do sample changes? And are
there any recommended vendors? Any other tips or suggestions would be
appreciated.

Thanks,

Joe Neilly
Abbott Laboratories
Cellular and Microscpic Research
Abbott Park, IL 60064
Phone: 708-938-5024
E-mail: neilly.joseph-at-igate.abbott.com






From: JOHNA-at-SCI.WFEB.EDU
Date: Wed, 10 May 1995 17:58:56 -0400 (EDT)
Subject: Re: sectioning glass

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Hello Colleen,

You will definitely get best results with a diamond knife. I found that a
35 degree knife gave the best results while sectioning 35 um dia. silica
beads. There was mininimal degradation of the knife when sections up to
0.5 um thick were cut. Sections thicker than this tended to hurt the knife
edge much more quickly. We also found that the "hard" formulation of
Spurr's resin was quite adequate as an embedding medium for these beads.

Hope this helps; good luck.

John

___________________________________________________
| |
| John G. Aghajanian, Ph.D. |
| Worcester Foundation for Experimental Biology |
| 222 Maple Avenue |
| Shrewsbury, MA 01545-2737 |
| |
| Tel: 508 842-8921 ext. 147, 161 |
| Fax: 598 842-9632 |
| JOHNA-at-sci.wfeb.edu |
| |
|_________________________________________________|





From: Gerry LITTLE :      ANGJL-at-medicine.newcastle.edu.au
Date: Thu, 11 May 1995 08:31:05 GMT +11
Subject: Reply to cryosectioning WAT

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G'day Jeanne,

I have not attempted sectioning WAT, but we were having similar
problems when sectioning sciatic nerve. The individual nerve fibres
would pull apart. This happened when using the standard pickup
procedure of a sucrose droplet. However we have found that using the
electrostatic transfer method as described by Tsuji et al., 1992
Arch. Hist. Cytol. 55:423-428, works very well. It may be similar
for you with WAT.
Hope this helps?
Regards,
Gerald.




Dr Gerald J. Little | Ph (61 49) 215618
The Neuroscience Group |
Discipline of Anatomy | Fax (61 49) 216903
Faculty of Medicine and |
Health Sciences |
The University of Newcastle | Email ANGJL-at-medicine.newcastle.edu.au
Australia, 2308 |




From: Olli Tahvonen :      tahvonen-at-are.Berkeley.EDU
Date: Wed, 10 May 1995 17:12:42 -0700 (PDT)
Subject: unsubscribe

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From: jpawley-at-macc.wisc.edu (James Pawley)
Date: Wed, 10 May 1995 21:36:41 -0600
Subject: Re: Photo-Subtraction, Are we all talking about the same thing?

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} I much prefer to keep the microscope clean, and not try to work around
} those "dust particles". I don't by any means work in a sterile environment,
} and there always are a very few in the field of view, but I opt for leaving
} them instead of going to heroic methods to clean them out. Also there is
} the stuff on the slide you are working on. Even though I prewash slides,
} a starch grain pops up from time to time. I would rather explain my
} opinion of what something in the field of view is, than take it out and
} have been wrong.
}
} I guess my view is clean is better than correction for dirt.
}
} So you know how crazy I really am, yes I also readjust the illumination
} when I swing the objective turret.
}
} Shalom from Jerusalem,
} Azriel

I may be wrong, but it seems that some are talking about image subtraction
in the context of video-enhanced contrast imaging. In this technique,
small features are made visible because even though at least one of their
dimensions is below the resolution limit of the LM, they still produce a
small amount of contrast (say 1%) in a DIC image. With suitable image
processing, (where suitable is defined as that which allows you to see
something useful about the specimen that you could not otherwise have
discerned.) this contrast can be made visible as long as the image contains
only a few such features and they do not overlap.

In this procedure Bob Allen and others showed that it wasn't just "dirty
optics" that produced background features in the enhanced-contrast image
that were unrelated to the specimen but but there were also more complex
sources such as inhomogeneities in the vidicon target and imperfections in
the optical surfaces and gluing that are not usually troublesome as they
cannot be seen without video-enhanced contrast. Subtracting such low
contrast features seems relatively innocuous (a 1% change at worst),
especially when they can be clearly shown NOT to be related to the
specimen. Subtracting a bare field image is a simple way to approximate
the removal of such features from the final data so that one can see the
specimen. (We should note also that though such a subtractive correction is
simple, it is not ideal. A better correction would involve both a
multiplicative and an additive/subtractive correction.)

This situation is very different to that of "subtracting" a high-contrast
feature such as a starch grain from an image. I hope that this helps.

Jim Pawley

***************NEW ADDRESS**************
Prof. James B Pawley, Ph. 608-263-3147
Room 1235, Engineering Research Building, FAX 608-265-5315
1500 Johnson Dr. Madison, Wisconsin, 53706.
JPAWLEY-at-MACC.WISC.EDU






From: GVKM07A-at-prodigy.com (DR CHARLES A GARBER)
Date: Wed, 10 May 1995 23:04:30 EDT
Subject: Sectioning glass fibers

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-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --

On May 10, Colleen Ann Lavin asked about the sectioning of glass fibers:

1] You will definitely need a diamond knife and you will not be able
to do this with a glass knife,

2] If your "old" diamond knife is too chewed up, you won't be able to
use it either, and

3] A "materials science" diamond knife, such as one of the SPI
Materials Science diamond knives, is just like (e.g. same angles, etc)
a "regular" life science knife but you are not paying for an edge that
is 100% completely free of fine striations. The SPI "Materials Science"
diamond knives are about 1/3 less in price than a "life science" knife
of comparable edge length, yet the few fine striations still left will
not cause you any problem whatsoever. For the cutting of glass fibers,
you will be putting striations into the knife edge that are more
profound than the fine ones we are talking about taking out, therefore
why pay a premium price for something that will give you no benefit?

4] We have made excellent blocks in our own laboratory of glass fibers
using our SPI #2660 SPI-Pon 812 resin kit which can be cured quite
hard. You might want to vacuum embed. I guess you would have to either
dehydrate the sample but another alternative might be to CPD the sample
and then embed. The advantage of CPD is that you could flash on some
gold, some of which might penetrate deep enough into theglass filter to
provide some kind of decoration between the cells and embedding medium.

5] I would guess that a fracture face and SEM examination would give
you something very hard to interpret.


Hope this is helpful in some small way!

Charles A. Garber
PRESIDENT
SPI SUPPLIES
PO BOX 656
WEST CHESTER, PA 19380 USA

Ph: (800) 2424-SPI
FAX:(610) 436-5755

e-mail: GVKM07A-at-prodigy.com







From: chris gilpin :      CGILPIN-at-fs2.scg.man.ac.uk
Date: Thu, 11 May 1995 8:33:01 GMT+1
Subject: subscribing

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Message-Id: {199505110617.CAA10547-at-interramp.com}

Hi all,
I seem to have lost the listserver Address. A colleague of mine wants
to join the list.
Many thanks


Chris






From: ntodd-at-unix.cc.emory.edu (Norman Wendell Todd, Jr, MD)
Date: Thu, 11 May 1995 07:54:00 -0500
Subject: unsubscribe

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Please unsubscribe.

N.W. Todd, Jr, MD
ntodd-at-unix.cc.emory.edu

_
:-)
-






From: SGKCCK-at-aol.com
Date: Thu, 11 May 1995 08:16:43 -0400
Subject: sectioning of glass

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Dear Colleen,
Regarding the cutting of glass fibers at 1micron average thickness please
note the following:
Do not go out and buy a new knife for this application especially if this is
a one time project that will not haunt you for the years to come. I
regularly(daily) cut glass beads for many customers and have been doing so on
my low angle (35 degree) which offers less compression, diamond knife. I
also noticed that John responded confirming what I am saying. If you do not
have a low angle I would simply use your standard 45 degree or even better
yet if you had a histo knife in the lab it would be perfect for 1 micron
thick sections and they are so cheap that if over time damage was caused it
is nominal to have the knife resharpened. The material science knife you
speak of is an absolute waste of money. You do not have to buy a special
knife for different types of materials.
If you are unsure, you can always send me your sample and as a free service I
will cut it for you like I do for all of my customers, give you a full report
including micrographs and samples on grids so you can see for yourself. I do
this for many customers on a regular basis and it really helps them to
eliminate all of the guess work.
Regarding embedding I would not worry about adherance problems(z-6040 by dow)
for these particles do not seem to be large where seperation of embeddment
and sample would occur. Just use plain old spurrs and you will be fine. No
need for any of the special prep work. Glass fiber is actually very easy to
work with and I truly do not see any difficulties ahead of you. Do not even
consider cryo for it is just going to complicate you and cause you more
problems than it is worth.
I remain at your disposal if you have any questions or you would like for me
to cut these samples for you without any charge.
Stacie Kirsch
Diatome U.S.
P.O. box 125
Fort Washington, Pa. 19034
Tel: 215-646-1478
Fax:215-646-8931




From: EMLAB-at-opus.mco.edu
Date: Thu, 11 May 1995 08:55:03 -0500 (EST)
Subject: Re: Converting to LaB6

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Joe,

We have used both FEI and Barry Scientific LaB6 cathodes in our
Philips 410LS operating at 80KV. Have had no major trouble with either brand.
Our scope did have a timer added to the saturation control but other than that
no modifications were done. We do not change specimens any different than with
a tungston filament. We do try and leave the HT on but in a low emmision mode,
when we are not actually using the scope. We have had up to 3000 hrs (according
to hour meter connected to scope) on a single LaB6 cathode. The main advantages
of a LaB6 are its increased brightness and much longer life, therefore fewer
column vacuum breaks. The main disadvantages is the fragility of the crystal
carbon mounts. When installing the cathode in the wehnalt, extreme care must
be taken so you do not snap the mount. One day I broke Two mounts, $1000.00
shot to hell in a matter of ten minutes. In conclusion I would recommend
purschasing a LaB6.

Best of Luck,

Ed Calomeni
Medical College of Ohio
Toledo, OH
emlab-at-opus.mco.edu







From: baskin-at-biosci.mbp.missouri.edu (Tobias Baskin)
Date: Thu, 11 May 1995 08:34:35 -0500
Subject: re: triple labelling of antigens...

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Greetings,
THough I have never tried any of these myself, there are examples
and recipees and suggestions for double and triple labelings in the recent
book:
Immunohistochemistry II. ed A.C. Cuello. Wiley 1993 (also known as
IBRO Handbook series: Methods in Neurosciences, Vol 14.

I believe that all of the examples in the book deal with neural tissue.

Hope this helps. (BTW, I have no commercial link/tie/gain from the book

Tobias Baskin






From: emccjb-at-ttuhsc.edu (Charles J. Butterick)
Date: Thu, 11 May 1995 08:34:35 -0500
Subject: Commercial Use of the List

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To: MICROSCOPY-at-AAEM.AMC.ANL.GOV

Greetings,
One of the real pleasures of the Microscopy List is the free
exchange of opinions with other microscopists. The combined wisdom is a
valuable tool. However, the replies of some vendors to the List cause me
concern, especially when brand names and catalog numbers specific to that
vendor are used. While a vendor's experience may be pertinent to a given
query, I wonder if this List is an appropriate venue to sell their
products.



Charles J. Butterick (Chuck)
Electron Microscopy Center
Department of Cell Biology
and Biochemistry
Texas Tech University Health
Sciences Center
3601 4th Street
Lubbock, Texas 79430

vox (806) 743-1633
fax (806) 743-1219
email emccjb-at-lubb.ttuhsc.edu or
chuck-at-micron1.lubb.ttuhsc.edu






From: Dr. Peter Steele :      70152.3105-at-compuserve.com
Date: 11 May 95 10:39:04 EDT
Subject: Muscle Embedding

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We routinely process muscle biopsies in Embed 812 (EMS) overnight and have them
on the scope by noon the next day. I am in agreement that the major problem for
Departments starting muscle pathology is that the pathologists often submit huge
pieces. Ensure that your specimens have at least one measurement that does not
exceed 1 mm (e.g., cross-section are no deeper than 1 mm, longitudinal sections
are no wider then 1mm) and most of your problems will resolve. If you require
methodology email me directly (not the listserver) and I will be happy to fax
you recipes and times.





From: sje-at-po.CWRU.Edu (Steven J. Eppell)
Date: Thu, 11 May 1995 11:28:10 -0400
Subject: Dye sub. printer problem

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Message-Id: {199505111528.LAA20796-at-robin.INS.CWRU.Edu}


We've been having some problems with our Tektronix
Phaser IIsdx dye sub. printer. Using four color transfer
rolls, we're getting streaks in the prints. The streaks run
along the long axis of the page and are denser at the top of
the page than the bottom. One of the transfer rolls showed
this streaking and a misalignment of the colors on the print.
Both of these problems go away when we change transfer
rolls. We've gone through 6 rolls and 2 of them have
displayed this streaking. Another roll was not even
recognized by the printer as a Tektronix transfer roll.
So, we're batting .500 with transfer rolls. Tektronix claims
that their quality control is much better than this and that
the problem must be somewhere else in our system. Has
anyone seen this type of behavior with a dye sub printer?
Do you have any clues as to what may be causing it?




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Thu, 11 May 1995 10:40:33 -0500 (CDT)
Subject: MSA Certification Board

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Some information about MSA Programs/Officers etc..
can be found on the Microscopy&Microanalysis WWW site,
(URL=http://www.amc.anl.gov)

Look at the section called On-Line Information about
National/International Societies...

MSA has a home page listed there.

From that source, here is the current contact person.

¥ Certification Board
-------------------------
Karen Klomparens (1995-97)
Center for Electron Optics
B5 Pesticide Research
Michigan State University
East Lansing, MI 48824-1311



Nestor
Your Friendly Neighborhood SysOp.





From: Tim Foecke :      tfoecke-at-NIST.GOV
Date: Thu, 11 May 1995 11:36:46 -0400
Subject: Re: Commercial Use of the List

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} Greetings,
} One of the real pleasures of the Microscopy List is the
} free exchange of opinions with other microscopists. The combined
} wisdom is a valuable tool. However, the replies of some vendors to
} the List cause me concern, especially when brand names and catalog
} numbers specific to that vendor are used. While a vendor's experience
} may be pertinent to a given query, I wonder if this List is an
} appropriate venue to sell their products.
}
}

I would agree. Responding directly to an inquiry is fine, but
cc'ing to the list is over the top.





From: John Phelps :      phelps-at-ENH.NIST.GOV
Date: Thu, 11 May 1995 09:49:20 -0600 (MDT)
Subject: darkroom effluents

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Hello Group,

Our current darkroom facility is under review by our safety inspection team.
The question is during the final rinsing procedures for negative and prints,
how much silver goes down the drain? We develop approximately 10 to 50
3.25" x 4" kodak SO163 negatives a month, and process maybe 10 prints from
these negatives per month. Does anyone have a feel for the silver that
might be in the effluents? Does anyone know the typical weight of silver
per square cm used on SO163 and printing papers?

thanks in advance,
John

John Phelps
NIST
Materials Reliability Division
Boulder, Colorado 80303
ph. 303-497-7570
fax: 303-497-5030




From: Ann-Fook Yang :      YANGA-at-EM.AGR.CA
Date: Thu, 11 May 1995 14:27:24 -0400
Subject: Wheat embedding

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Message-Id: {sfb21ea8.061-at-EM.AGR.CA}
X-Mailer: Novell GroupWise 4.1

I am working on a project requiring fixing and embedding wheat
seeds. My preliminary trial by embedding them in Epon, Spurr's and
Lowicryl K4M failed to infiltrate the tissues properly especially
endosperm. Soaking the seeds for one day improved somewhat but was
unsatisfactory. Can anyone help? TIA.

Ann Fook Yang





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Thu, 11 May 1995 14:34:48 -0500 (CDT)
Subject: Dye Sub Problems

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I've been using a Phaser IISDX for several years. Never
had that problem. It may sound silly, but there is a
cleaning procedure which Tek. recommends. Have you done
that to all appropriate surfaces??? It should be
documented in one of the manuals. I've only ever done
it once, when the colors were coming out inconsistent
(i.e. fading after successive prints). Once cleaned I
never had the problem again.

Nestor
Your Friendly Neighborhood SysOp.




From: cook-at-aaem.amc.anl.gov (Russell E. Cook)
Date: Thu, 11 May 1995 12:59:42 -0600
Subject: Correction to converting to LaB6

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I was incorrect in my statement about LaB6 vesus CeB6 in our JEM-4000EXII.
Sorry about that: I am not responsible for that microscope. In fact, we
use an FEI CeB6 in a compression mount. That explains why carbon
contamination is not a problem.

Russell E. Cook
Electron Microscopy Center for Materials Research
Argonne National Laboratory
9700 South Cass Avenue
MSD-212
Argonne, IL 60439
(708)252-7194
FAX: (708)252-4798






From: jerry-at-biochem.dental.upenn.edu
Date: Thu, 11 May 1995 15:23:25 -0400
Subject: vendors

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I also have felt uncomfortable recently with vendors "hawking their
wares" over the general listserver, although, I have benefitted in the past
when vendors reached me directly with product information including price
and availability. I would like them to continue to monitor the Microscopy
Listserver for such inquiries and answer them directly as I may need such
information in the future.

Thanks--Jerry





From: GANTZ-at-med-biophd.bu.edu
Date: Thu, 11 May 1995 15:35:44 -0500 (EST)
Subject: Converting to LAB-6

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Dear Joe N.,
We converted our CM-12 to LAB6 about a year ago and so far over 1000 hours
of saturated time. Purchased Kimball Physics, Inc. cathode through Barry
Scientific-both companies very helpful. We've learned a lot about care and
Generally, unsat. HT should be on at all times except changing plates to
prevent oxide formation. Don't saturate until IGP reading below 25.
Willing to discuss details if you give me a call.
Donald Gantz
Boston Univ School of Medicine
617-638-4017
E-Mail: gantz-at-med-biophd.bu.edu




From: Larry Allard :      allardlfjr-at-ma160.ms.ornl.gov
Date: 11 May 1995 13:58:34 U
Subject: Thanks Nestor

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Message-ID: {n1411921592.24647-at-ma160.ms.ornl.gov}

Subject: Time: 1:50 PM
OFFICE MEMO Thanks Nestor Date: 5/11/95

Nestor:
Now that the listserver appears to be functioning normally after the recent
problems, I just wanted to express thanks for your efforts in getting
everything back in operation. I speak for others at my lab, and I am sure
for others elsewhere, who realize what a job it must be tending this server
that we find so valuable. Now, I hope this does not generate a lot of "ditto
for me" messages, as they will just add to the message load (hint to other
subscribers) and may cause you additional headaches. Just Thanks again for
the good job.

Larry





From: EMLAB-at-opus.mco.edu
Date: Thu, 11 May 1995 15:30:59 -0500 (EST)
Subject: Re: Commercial Use of the List

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In my opinion I feel no reason to get upset with a supplier telling
about their products. If we (the consumers) can tell good/bad stories
about certain products when asked by someone on this newsgroup, why should a
vendor not be able to do the same thing???? Of course this does not mean open
warfare between various suppliers about products. At first I was a little
concerned about the suppliers "hawking" their wares but after some
consideration I felt it was ok. The vendors in question did not try to sell
anything to anybody, but replied to a specific question, granted, with their
products. Is this any different than a person trying to sell a used microscope,
which has happened many times????

My 2cents worth

Ed Calomeni
Medical College of Ohio
Toledo, OH
emlab-at-opus.mco.edu




From: WINSTON_VERN :      winsvern-at-cwis.isu.edu
Date: Thu, 11 May 1995 13:16:09 -0600 (MDT)
Subject:

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I am in charge of maintenance of an EM facility which includes
two ultramicrotomes (an RMC MT7 and an older Sorvall MT2-B). I would be
interested to know how other labs maintain instruments like these?
Service contracts? Demand service? Which companies have been reliable?
Is it possible for a reasonably careful person to do their own maintenance?

As you may have guessed, I am having troubles obtaining service
from my present provider, but I don't want to go into my own problems on
the net. Suffice it to say that anything is better than the service I am
getting now (and paying good money for). Any suggestions would be
welcome, either on this forum or via email.

Thank you in advance

Vern Winston
Department of Biological Sciences
Idaho State University
Pocatello, Idaho 83209-8007 winsvern-at-isu.edu






From: baskin-at-biosci.mbp.missouri.edu (Tobias Baskin)
Date: Thu, 11 May 1995 17:31:51 -0500
Subject: more on vendors

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Alternate-Recipient: allowed
Auto-Forwarded: prohibited
Content-Return: allowed
Disclose-Recipients: prohibited
Conversion: allowed
Importance: normal
Priority: normal
Sensitivity: Company-Confidential
WALCKSD-at-ml.wpafb.af.mil
Message-Id: {950511165554.562-at-cliff.ml.wpafb.af.mil.0}

Greetings,
I think that is is worth making a distinction between vendors who
reply to a question posed on the net and vendors who spontaneously announce
products for sale. The latter is simply advertising and I feel has no place
here. But, I have no problem with a vendor replying to a query about
something with product info, provided that the vendor clearly identifies
themselves as such. Several folks have said they think the vendors should
correspond directly with the original questioner but many of us often have
the same question and are interested in the replys. We use the subject line
as a filter.
Just my two cents.

Tobias Baskin






From: kleifer-at-cimedec1.epfl.ch (Klaus Leifer)
Date: Thu, 11 May 1995 15:47:21 +0000
Subject: Security during TEM preparation of GaAs and InP

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Message-Id: {abd7f8b600021004600f-at-[128.178.98.24]}
Mime-Version: 1.0
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To: Microscopy Mailing List {microscopy-at-aaem.amc.anl.gov}

As in lots of EM laboratories we prepare TEM specimens from GaAs and InP
bulk material and thin films .

From our lab and other labs we know that the precautions for the people
preparing the specimen and the treatment of the water used during the
different steps of the preparation are very different.

Which precautions do you take? What do you consider as necessary?

Thanks in advance
Klaus Leifer

_________________________________________________________________
***** ***** ***** * Klaus Leifer
* * * * * Ecole Polytechnique Federale de
Lausanne
**** ***** **** * Centre Interdep. Microscopie Electronique
* * * * 1015 Lausanne, Switzerland
***** * * ***** Phone:+41(21)6934830 Fax:+41(21)6934401
_________________________________________________________________








From: jfmjfm-at-umich.edu (John F. Mansfield)
Date: Thu, 11 May 1995 14:57:43 -0400
Subject: Re: Commercial Use of the List

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A little Netiquette (mailing lists or newsgroups):

If someone asks a question that may or may not be of general interest to a
wide audience, the standard way of replying to that question is to send
email to originator.
If you are interested in the replys that that person gets you send a
request to them asking for copies of the replies.
If the originaor receives several requests for copies of the replies (s)he
may decide that a summary of the replies should be posted to the
list/newsgroup.

OK?


John Mansfield
North Campus Electron Microbeam Analysis Laboratory
413 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (313)936-3352 FAX (313)936-3352
jfmjfm-at-engin.umich.edu, John.F.Mansfield-at-umich.edu
or jfmjfm-at-umich.edu they all reach me!
URL: http://www.engin.umich.edu/~jfmjfm/jfmjfm.html






From: Beth Richardson :      beth-at-dogwood.botany.uga.edu
Date: Thu, 11 May 1995 17:46:36 -0400 (EDT)
Subject: Re: Wheat embedding

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During the initial fix we use a drop or two of photo-flo as a wetting
agent - plant material tends to be hydrophobic. We also cut the tissue
into small pieces - this may not be ok for what you want to do. We
use Spurrs and do 3 changes in 100% for at least 8 hrs/change. Sometimes
it works and sometimes it doesn't. As you already know seeds are tough to
work with - use very young, fresh material if possible.

Good luck!

Beth Richardson
EM Lab Coordinator
Botany Dept.
University of Georgia
Athens, GA 30602-7271
(706) 542-1790

On Thu, 11 May 1995, Ann-Fook Yang wrote:

} I am working on a project requiring fixing and embedding wheat
} seeds. My preliminary trial by embedding them in Epon, Spurr's and
} Lowicryl K4M failed to infiltrate the tissues properly especially
} endosperm. Soaking the seeds for one day improved somewhat but was
} unsatisfactory. Can anyone help? TIA.
}
} Ann Fook Yang
}
}




From: SALLY STOWE :      STOWE-at-rsbs-central.anu.edu.au
Date: Fri, 12 May 1995 11:43:40 EST10
Subject: yet more on vendors

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another two cents worth -
I agree with Tobias Baskin - a vendor replying to a query often
provides useful information. Apart from that, vendors are microscopists
too, often pretty good ones, probably even bleed if they are cut
etc......
cheers,
Sally Stowe

----------------------------------------------------------------------
Sally Stowe Australian National Univ.
Facility Coordinator Canberra, AUSTRALIA
ANU Electron Microscopy Unit Ph 61 6 249 2743
RSBS, Box 475
Email stowe-at-rsbs-central.anu.edu.au FAX 61 6 249 4891
------------------------------------- --------------------------------
-





From: Jay Jerome :      jjerome-at-isnet.is.wfu.edu
Date: Thu, 11 May 1995 15:01:12 -0400 (EDT)
Subject: Re: Commercial Use of the List

Contents Retrieved from Microscopy Listserver Archives
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I find some of the commercial information useful and would hate to see it
cc'd only to the inquirer rather than to the list. I guess there is a
fine line between information specific to a product and blatant
advertising. I would hate to see the list become commercialized, but I do
think vendors have information to impart that is both useful to their
desire to sell product and is useful to end users need for info. I don't
have an answer, just a hope we won't inhibit info exchange because it is
somewhat commercial.

Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-isnet.is.wfu.edu *
**************************************************************

On Thu, 11 May 1995, Tim Foecke wrote:

} } Greetings,
} } One of the real pleasures of the Microscopy List is the
} } free exchange of opinions with other microscopists. The combined
} } wisdom is a valuable tool. However, the replies of some vendors to
} } the List cause me concern, especially when brand names and catalog
} } numbers specific to that vendor are used. While a vendor's experience
} } may be pertinent to a given query, I wonder if this List is an
} } appropriate venue to sell their products.
} }
} }
}
} I would agree. Responding directly to an inquiry is fine, but
} cc'ing to the list is over the top.
}
}




From: lmiller-at-ux1.cso.uiuc.edu (Lou Ann Miller)
Date: Thu, 11 May 1995 17:21:02 -0600
Subject: Agenda,May 19th, Central States Meeting

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Hello,

Everyone is invited to attend the Central States Microscopy Society Spring
Meeting.


Friday, May 19th at the College of Veterinary Medicine, University of Illinois.

Times: 8:20-5pm

Registration: Free, 7:45-8:20am, can come later. Lunch is $5

Includes: 2 simultaneously running sessions, one mostly
material sci, other biology
Student Presentation Competition, 2 Demo's,
Venders, and Tours of the
U of I facilities for microscopy

Session 1:
Microscopes of the Future Dr. Alwyn Eades

Characterization of Corrosion Scales in Water Pipes Using
Electron Microscopy Jim Shull*

Constrained Melt Polymerized Crystals of a Family of Random
Ter-Polyesters. Clara Gonzalez*

Hydrogen effects on Dislocation Motion Paulo Ferreira*

Ion Implantation Damage in the AlGaAs/GaAs System
Britt A Turkot*

Effect of Hydrogen on the Deformation and Fracture
Behavior of a Beta-Titanium Alloy David F Teter*

Study of Copper-Polyimide interfaces
Marlon E Menezes*

Dynamical Calculation of RHEED Patterns From Rough Silicon 001 Surfaces
Scott Lordi*

Monitoring Air Pollutants in Lichens by Means of Microscopy
and X-Ray Microanalysis Dr. Richard Crang

Localization of the Dioxin Receptor: Human Health Implications
Dr. Paul Cooke

Cellular Localization of a Major Acid Phosphotase in
Francisella tularensis Dr. Mark Kuhlenschmidt

An In Vivo Model of Podocyte Charge Alteration and Early
Glomerular Injury Dr. Howard Gelberg

Session 2:

Immunogold Localization of Cytochrome-C Isoforms
in Testicular Germcell Mitochondria--Dr Rex Hess

What's on the Information Superhighway for
Microscopists? Dr. Ron Smith

SEM and TEM Characterization of Protein
Microspheres. Mike Wong*

Biodistribution of I.V. Injected Protein Microspheres
Ken Kolbeck*

Identification of Specific Relaxin-Binding Cells in the
Cervix of the Pregnant Pig utilizing a Biotinylated
Porcine Relaxin Probe Min Gyesik*

Cytoskeletal Organization of Factors Involved in their
Function Dr. Heidi Schatten

Use of Methylmethacrylate For Plastic Casting of
Kidney and Liver Vasculature Dr. Kenneth Holmes

Digital Imaging
Dr. Bridget Carragher


Demo's:

Microwave Demo
Lou Ann Miller

Demo's in MW oven calibration, tissue fixation
and thin section grid staining in Uranyl Acetate
This is an informal group demo/ discussion,
participants are welcome to bring their own
photo's and Procedures.

Ted Pella Rep. Lee Dickey will have some
MW procedure information and a
Demo of their latest Microwave model.
.............................................

Computer Software Usage for 2D & 3D
Image Manipulation
Janet Hanlon

Histology Images are used with software
Programs like Photoshop, and more.
This is an informal demo/ discussion group.
Participants are encouraged to bring Questions
or even a DOS formatted disk with an image.

............................................................

Tours:
Bevier Center for Electron Microscopy
MRL--Material Research Lab
Beckman Vis Lab--EM and Digital Imaging

For more information or to be sent a schedule/ maps etc,

Contact:

Lou Ann Miller
lmiller-at-ux1.cso.uiuc.edu
217-244-1566 (7am-4pm CST)
Fax: 217-333-4628


***********************
Lou Ann Miller
Microscopic Imaging Laboratory
College of Veterinary Medicine
University of Illinois
2001 S Lincoln Ave
Urbana, Illinois 61801
217-244-1566
lmiller-at-ux1.cso.uiuc.edu
***********************






From: cook-at-aaem.amc.anl.gov (Russell E. Cook)
Date: Thu, 11 May 1995 12:50:38 -0600
Subject: Re: Converting to LaB6

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Message-Id: {v01510100abd7e4839a76-at-[146.139.72.78]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Joe Neilly of Abbott Laboratories asked:
} We are planning to convert the tungsten filament in our Philips CM-12
} STEM to a LaB6 source. I am aware of the general precautions one must
} take with regards to vacuum and saturation to preserve the LaB6 crystal
} but not the details. I would like to know what specific precautions CM-
} 12 users take when using their instruments with a LaB6 filament. Is the
} built in saturation delay of the instrument slow enough? What vacuum is
} low enough to begin saturation? How do you do sample changes? And are
} there any recommended vendors?

I compared the operating characteristics of 3 thermionic emitters (W, LaB6,
and CeB6) in Philips TEMs and reported the results at last year's MSA/MAS
meeting in New Orleans (abstract in the MAS proceedings). Here are some of
my conclusions:

I. LaB6 or CeB6 crystals with 90 degree cone angles will be 3X brighter
than standard W loops when the hexaboride emitters are heated to the point
at which the beam current and the source image are no longer change
significantly (the source images have a slight bit of detail in it at that
point). All of the emitters were mounted identically in the Wehnelt
cylinder, i.e. 0.2 mm back from the Wehnelt's outer surface which had a 0.5
mm aperture, and were biased identically (maximum bias, minimum "emission
number").

II. Hexaboride emitters with 60 degree cone angles will be 12X brighter
than standard W loops when the above conditions are satisfied.

III. Not all emitter/gun combinations are satisfactory:

A. For instance, CeB6 crystals in compression mounts had typical lifetimes
of 720 hours in our Philips TEMs before cracks developed in the crystal
volume between the compression mount structures. The crystals then became
tilted in or fell out of the mounts. The cracks may form because of
frequent thermal cycling: our standard operating procedure is to cool a
LaB6/CeB6 cathode before inserting or removing samples to reduce the risk
of degrading the emission properties by contamination (Philips TEMs have no
gun isolation valves which the user can operate). That is, inherent flaws
in the crystals, whether LaB6 or CeB6, are caused to grow under
compressive, cyclic stresses. However, LaB6 crystals in a wire-supported,
brazed mount do very well in our Philips guns: typical useful lifetimes
are over a year.

B. On the other hand, LaB6 crystals in compression mounts work quite well
in our JEOL JEM-4000EXII. The voltage is always on although the cathode is
heated and cooled each day. The gun can be isolated from the column.
However, LaB6 crystals in a wire-supported, brazed mount do very poorly in
this gun: their brightness decreases rapidly in the first week. We think
that these crystals may become contaminated with carbon or something else
(LaB6 cannot recover its original emission properties after carbon
contamination, but CeB6 does recover). We don't understand why carbon from
the graphite in the compression mounts does not contaminate the LaB6.

IV. As for the specific operating procedures in our Philips TEMs (EM420T
and CM30T), we insert the sample with the HT off, wait until the vacuum is
3E-7 torr or 4E-5 Pa, turn on the HT, and heat the cathode to "saturation"
in three minutes (a modification of an old DENKA recommendation). We
instruct users to "saturate" the cathode while observing the source image
rather than simply increasing the filament control to some predetermined
number to prevent over-saturating the cathode and to maintain better
control over the gun tilt (tilt changes for different voltages and cathode
temperatures). The filament control must be decreased over the first 30 -
60 minutes because the cathode continues to heat slowly beyond the
"saturation" point during that time. Before changing samples, the cathode
is cooled over 3 minutes, the HT is shut off, and the user waits 5 minutes
before removing the sample holder. We are very cautious in this procedure
because of the lack of the ability to isolate the gun.

V. The filament and emission (bias) controls can be varied to achieve
maximum brightness, etc. Contact me for details.

VI. We have been using DENKA 90 degree LaB6 crystals in a wire-supported,
brazed mount in the Philips TEMs. Recently, we have been using FEI 90
degree LaB6 crystals in compression mounts in the JEOL JEM-4000EXII.

Russell E. Cook
Electron Microscopy Center for Materials Research
Argonne National Laboratory
9700 South Cass Avenue
MSD-212
Argonne, IL 60439
(708)252-7194
FAX: (708)252-4798






From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Thu, 11 May 1995 10:50:40 -0500 (CDT)
Subject: Reminder on Ground Rules

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G'day Subscribers....

Yes, I've noticed a few (not all) users are starting to
stretch the limits... for the most part things have
been on the up and up. So, let me just repost a portion of
the ground rules that everyone first received when
you subscribed...

Thanks in advance for everyone's co-operation.

Nestor


===============================================================================

General Ground Rules
on using the ANL AMC
Microscopy Listserver/Mailreflector

Before continuing you should understand your responsibilities as a
Microscopy Listserver/Mailreflector System user.

Specifically they are:

1.) Actively encourage and promote the free exchange and discussion of
information, ideas and opinions, except when the content would
compromise the national security of the United States (or any other
country for that matter) ; violate proprietary rights, personal privacy,
or applicable state/federal/local laws and regulations affecting
telecommunications; or constitute a crime or libel.

2.) Use your REAL NAME and fully disclose any personal, financial, or
commercial interest when evaluating any specific product or service,
or contribution.

3.) Do not use this system for delivery of personal mail, messages
or items of a similiar nature (i.e no posting of resume's ,
or overt commericalism i.e. selling products.. etc....)
If you are not sure then it probably does not belong here!
If you would like an opinion then Email the message to me and I will
review and comment as appropriate. (Zaluzec-at-aaem.amc.anl.gov).

4.) Adhere to these rules and notify the Listserver-at-aaem.amc.anl.gov
immediately when you discover any violations.



-----------------------------------------------------------------------------


**************************************
* FAQ's File *
* (Frequently Asked Questions) *
*Microscopy/Microanalysis Listserver:*
**************************************
* Last Updated April 11, 1995 *
**************************************



What can I post?
----------------

Basically any question/comment/observation
or general information/announcement which involves
microscopy and/or microanalysis. This list is
not moderated, so it is up to the users to
self-police themselves. I generally take an openminded
attitude toward questions. If in my opinion, it appears that
a discussion is straying far off the limits of the intent of
this forum I will post a note to the group. But
I prefer to err on the safe side with most messages
and allow at least a modicum of generally. Clearly,
generic questions about Word Processing do not belong
in this discussion forum, so just use common sense.

If there is any question in your mind about
something you wish to post, send it to me first
at Zaluzec-at-aaem.amc.anl.gov and I will give you
my opinion/comments.


Can I post an Announcement of a Job Opening or a Meeting?
---------------------------------------------------------

Yes that falls within the bounds of the subject of this list as long
as it is related to Microscopy/Microanalysis.

Can I post my Resume'?
---------------------

No. This forum was not created for that purpose.
For the time being, you may post your resume on the MSA BBS system.


Can I post an Advertisement?
----------------------------

No, that does not fit within the bounds of this forum.

This listserver is not intended to be a Sales mechanism
for commerical organizations. If you are an
organization and have equipment you wish to donate,
or sell, for nominal cost (i.e. no profit) then this is generally
an acceptable posting. If you are not sure then send a
copy of the announcement in question to Zaluzec-at-aaem.amc.anl.gov
and I will give you my opinion. An example of this type
would be an old decommissioned instrument which someone
is trying to give away for removal/shipping costs, that would
fit within the bounds of the purposes of this list.


If you are a manufacturer, you are welcome to observe/join
in any discussion at any time. But, please refrain
from overt sales pitches and/or commericalism. If a product
which you produce can solve a problem or answer aquestion
raised by anyone, then by all means feel free to
say so briefly and then offer to continue the discussion with
any interested parties off-line. Just add your phone number
or Email address to the end of your message, any you'll
be contacted. Please keep your comments about any product
you "sell" to a minimum.


When commenting about any product/question it is
always appropriate to state your organization
and affliation.



*********************************
Nestor J. Zaluzec
Your Friendly Neighborhood SysOp.

*********************************
End of File
*********************************




From: MR RHM CROSS :      EURC-at-giraffe.ru.ac.za
Date: Fri, 12 May 1995 08:28:40 GMT+0200
Subject: commercial use of listserver

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As a subscriber to the listserver from one of the more remote parts of
the world where we do not have frequent opportunities to see and
discuss vendors' products, I find the information supplied by vendors
in answer to subscribers' enquiries to be very useful. It would be a
pity, therefore, if they were not permitted to use this means of
communicating with their customers or were restricted to replying
only to the originators of the enquiries.

They do not appear to be abusing the listserver at present but if
they should begin to do so I am sure that adequate means are
available to suppress their enthusiasm!

Robin Cross
Director : EM Unit, Rhodes University, Grahamstown, South Africa.




From: John Millar :      JJMILL-at-bunyip.ph.rmit.edu.au
Date: Fri, 12 May 1995 19:18:08 EST-10
Subject: Re: commercial use of listserver

Contents Retrieved from Microscopy Listserver Archives
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I agree with Mr. Cross
jjm


} From: "MR RHM CROSS" {EURC-at-giraffe.ru.ac.za}
} Organization: Rhodes University
} To: microscopy-at-aaem.amc.anl.gov
} Date: Fri, 12 May 1995 08:28:40 GMT+0200
} Subject: commercial use of listserver
} Priority: normal

} As a subscriber to the listserver from one of the more remote parts of
} the world where we do not have frequent opportunities to see and
} discuss vendors' products, I find the information supplied by vendors
} in answer to subscribers' enquiries to be very useful. It would be a
} pity, therefore, if they were not permitted to use this means of
} communicating with their customers or were restricted to replying
} only to the originators of the enquiries.
}
} They do not appear to be abusing the listserver at present but if
} they should begin to do so I am sure that adequate means are
} available to suppress their enthusiasm!
}
} Robin Cross
} Director : EM Unit, Rhodes University, Grahamstown, South Africa.
}
Professor John J. Millar
Head, Department of Applied Physics
Electron Microscope Unit
RMIT, GPO Box 2476V, MELBOURNE AUSTRALIA 3001
+613 660 2602 fax 613 660 3837
email jjmill-at-rmit.edu.au




From: dietmar.reiter-at-uibk.ac.at (Dietmar Reiter)
Date: Fri, 12 May 1995 12:55:03 +0100
Subject: Re: Commercial Use of the List

Contents Retrieved from Microscopy Listserver Archives
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} It strikes me as odd that scientists expect vendors to spend incredible
} resources in developing products and services to meet their needs, but when it
} comes to free exchange of information on a listserver like this we become
===========
} persona non grata. Vendors offer a very valuable service to the scientific
} community. Vendors and scientists should work together for their mutual
} benefit. People seem to think we're dirty because we are a "for profit"
} organization. Well, I'm here to say that VENDOR is not a dirty word and that
} vendors who contribute to this listserver should be commended for their
} interest
} rather than castigated for their responses.

I very much agree with David Henriks in that commerical vendors do
contribute to the academic community in a lively way. *List-restrictive*
people might be afraid from being OVERFLOODED with unselective, unprecise,
and otherwise plain advertisement material (as we are all used to be from
other media), which does not answer questions or contributes in a specific
way to a certain thread.
As this phenomenon is not the major part of communication on this and other
lists, I appreciate a concise form of product information that of course
comes with scientific research.

:-)

+++ Dietmar Reiter, Dept. of Zoology and Limnology
+++ University of Innsbruck
+++ Technikerstrasse 25, A-6020 Innsbruck, Austria
+++ phone: (43)-512-507 ext. -6170, fax ext. -2930






From: cjwood-at-dow.com (Charlie Wood 6-4510)
Date: Fri, 12 May 1995 09:01:53 -0400
Subject: Re:O in SiO2 Standard

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Alfred,

I am assuming that your SiO2 is carbon coated. The lower counts observed while
moving the beam may simply be the effect of the carbon coating. The MAC for O
by C is significant, around 12,000. If you continuously monitor your O counts
you will probably find that the count rate is a little low until the electron
beam literally 'burns' a hole in the carbon coat (30-60 sec) then reaches a
maximum which is stable until the carbon contamination starts to influence the
measurement.

Charlie Wood
Dow Chemical Co.
Midland, MI
cjwood-at-dow.com




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Fri, 12 May 1995 7:31:44 -0500 (CDT)
Subject: Enough of Commerical Use.....Nestor

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Okay folks, I think by now we have seen most variation on
opinions. Rest assured if I feel things are getting out of
hand I will send a note to the appropriate individual(s).

I'm happy that everyone feels that the VENDORS belong here
because I also believe this, and that short descriptive
info on their product which answers a question IS appropriate.

Let's drop the thread now.....

Nestor




From: Alan Pooley :      pooley-at-ahab.rutgers.edu
Date: Fri, 12 May 1995 10:07:00 -0400
Subject: Apology for posting of May 9

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Dear Microscopy net users,

I wish to apologise for the posting I made on May 9:
} "All equipment here is available to users, for a fee, with or without
} expert assistance. I have handled all kinds of SEM & EDS and would be
} willing to consult or work with any form of sample."

I had intended the posting to go ONLY!!! to one individual who
had requested contributions to an academic resources list. I did
not intend it to go to the microscopy group as a whole nor did I intend
any suggestion of commercial availability. I was trying to gain access to
listings of other academic microscopy resources by offering to contribute
my own listing. I did not intend to indicate availability of my equpment
or services except to academic users.
I apologise for my mistake in making the posting general and for
any unintended implication of commercial availability.


Alan S. Pooley, PhD Marine and Coastal Sciences SEM Lab
Rutgers University, New Brunswick NJ 09803-0231




From: DDKJoe-at-aol.com
Date: Fri, 12 May 1995 10:25:54 -0400
Subject: Re: sectioning glass

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I'm not sure you need another diamond knife vendor's opinion here, but I can
address the issues quickly.

A) By all means, use your old knife. This is always the way to begin with
samples you've never cut before. If you have some question about its
condition, we can inspect it for you at 800X at no charge. This will tell
you where you have the best chance for success.

B) Cryo probably won't help you here. Speaking for DDK and old DuPont
knives, cryo work and/or solvents will not degrade the epoxy.

We also offer free sectioning evaluations using your samples with grids,
micrographs, reports, etc. To arrange for this or discuss your project
further, give me a buzz.

Joe Tabeling
Delaware Diamond Knives
3825 Lancaster Pike
Wilmington, DE 19805
800-222-5143




From: gkrichau-at-unlinfo.unl.edu (Gary Krichau)
Date: Fri, 12 May 1995 10:06:55 -0400
Subject: 4pi SEII/EDS Board

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Message-Id: {9505121505.AA22010-at-unlinfo.unl.edu}
X-Sender: gkrichau-at-unlinfo.unl.edu
X-Mailer: Windows Eudora Version 1.4.4
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Does anyone have a board that they would be willing to loan for four
weeks? Wehave one on order but it won't arrive for another four week and we
have a little bit of a time problem with some research deadlines. We have
an image only board that we could trade for the duration.

Thanks in advance,
Gary Krichau

Central Facility for Electron Microscopy
University of Nebraska-Lincoln
___________________________________________





From: James Wesley-Smith :      WESLEYSM-at-biology.und.ac.za
Date: Fri, 12 May 1995 17:41:40 +0200 (SAST)
Subject: Re: Wheat embedding

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Message-Id: {199505121452.KAA05065-at-hoh.mbl.edu}

Dear Ann-Fook
We have often encountered difficulties whenever seeds at low moisture
contents are embedded. However, I don't think this is necessarily
related to the resin infiltration, but one of fixation in the first
place. It is quite likely that membrane conformation in the 'dry'
state may be retained as the fixative comes into contact with it.
Consequently, chemical penetration (including that of the fixative(s)
will not be as good as that of seeds which have been hydrated for
anything in excess of 4 hrs. So, if hydration is not a problem this
is the way to go. (Certainly making an incision along one side of the
area to be embedded and/or reducing the size of the specimen helps
the whole process.) However, if you need to look at the dehydrated
state I suggest you try freeze substitution although you will be
restricted in the size of the samples processed.

I hope this helps you.

James Wesley-Smith
EM Unit. Univ of Natal
Durban
South Africa











From: gwerdos-at-gnv.ifas.ufl.edu (Greg Erdos)
Date: Fri, 12 May 1995 12:56:49 -0500 (EST)
Subject: Lab6

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I have been considering switching to LaB6 too. What is the experience of
Hitachi H-7000 users out there in this regard.
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Greg Erdos Phone: 904-392-1295
Scientific Director, ICBR EMCL
218 Carr Hall Fax 904-846-0251
University of Florida E-mail: gwerdos-at-gnv.ifas.ufl.edu
Gainesville, FL 32611





From: Michael Cammer :      cammer-at-aecom.yu.edu
Date: Fri, 12 May 1995 12:42:41 -0400 (EDT)
Subject: Re: [Tim Foecke : Re: Commercial Use of the List]

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So far we have not been inundated with advertisements that have nothing to
do our fields of interest. We would find interesting and useful posts whereby
one vendor says, "Our product is better than the competition's AND THIS IS
WHY." Or, "This is how and why our product can help resolve the issues
you queried on the net." I would say, let's err in favor of not
segregating based on commercial or academic affiliation. When a sales rep
posts, "Buy our instrument and we will throw in a trip to the Bahamas,"
then we can tighten the rules.
Michael Cammer

} can forever erase any message that offends your style. I find the
} inclusion of commercial vendors a great asset for us "users". With
} their input maybe I can find out about a product or service, maybe I can
} get a new approach to a problem, and maybe I can even learn about their
} personal knowledge and interest in our endeavors.
} I say let them continue.






From: Ann-Fook Yang :      YANGA-at-EM.AGR.CA
Date: Fri, 12 May 1995 14:55:58 -0400
Subject: Schematics

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Message-Id: {sfb377eb.019-at-EM.AGR.CA}
X-Mailer: Novell GroupWise 4.1

We have a Matrix Multicolor Image Recorder (Color Image Recorder,
CIR-340, Nipon Avionics Ltd. Made in Japan). The address of Matrix
Instruments Inc. in the manual was Orangeburg, NY. The unit is
about 6 years old and in need of repairs. The supplier in Canada
cannot provide the service. We are told that the company went
under.
We have dedided to do our own repairs. I would appreciate any
leads towards obtaining a copy of the schematics. TIA.

Ann Fook





From: Fred Hayes :      fahayes-at-ucdavis.edu
Date: Fri, 12 May 1995 13:35:03 -0700 (PDT)
Subject: immunobed sectioning

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I am cutting a very small human diabetic epirentinal membrane (1.5 X 2.0mm)
embedded in Immuno Bed resin (size 00 beem capsules) with a block face of
1.0 X 1.5mm. Customer wants 20 slides, unstained, each with several
sections, located centrally on the slide (single drop), at 3 micron
thickness. Customer wants to label FGF with an antibody at the light level.
Immuno Bed is a very soft resin for light microscopy only.

I am cutting the sections without any problems on a dry glass knife. I
can also pick up the sections without any problems with a fine forcep.
The problem occurs in transferring the section to the glass slide. Is
there any way to increase the surface tension of the water droplet on
the glass slide causing it to bead? This will aid in the transfer and
flattening of the section. I could use a coated slide, but I'm
wondering if this could adversly affect the immuno results. (ie;
increase in background noise, affinity for dirt and other
contamination, skewed results, false positives, etc.)

Fred A. Hayes 916-752-7712 work
University of California,Davis 916-752-4701 work
School of Medicine
Department ofMedical Pathology; EM Lab
MSIA E-mail:
Davis, CA 95616 fahayes-at-ucdavis.edu

1320 Dogwood Court 916-678-6280 home
Dixon, CA 95620-3227







From: Fred Hayes :      fahayes-at-ucdavis.edu
Date: Sat, 13 May 1995 10:12:00 -0700 (PDT)
Subject: Re: Wheat embedding

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On Thu, 11 May 1995, Ann-Fook Yang wrote:

} I am working on a project requiring fixing and embedding wheat
} seeds. My preliminary trial by embedding them in Epon, Spurr's and
} Lowicryl K4M failed to infiltrate the tissues properly especially
} endosperm. Soaking the seeds for one day improved somewhat but was
} unsatisfactory. Can anyone help? TIA.
}
} Ann Fook Yang
}
}
Dear Ann,

Have you done a literature search? Try infiltrating in 1:1 Spurr's/200
proof ethanol overnight under a light vacumm (15-20psi). Next day, switch
to 100% Spurr's, and infiltrate under vacumm for two or three days using
a resin change each morning. The pot life of this resin can be varied for
up to 5 days or greater. Thats the great advantage of it, besides it's
low viscosity. You could also leave it in LR White at 4 degrees
centrigrade for weeks or even months. Good luck.

Fred A. Hayes 916-752-7712 work
University of California,Davis 916-752-4701 work
School of Medicine
Department ofMedical Pathology; EM Lab
MSIA E-mail:
Davis, CA 95616 fahayes-at-ucdavis.edu

1320 Dogwood Court 916-678-6280 home
Dixon, CA 95620-3227







From: m.dickson-at-unsw.edu.au (Melvyn R. Dickson)
Date: Mon, 15 May 1995 11:23:42
Subject: Re: Lab6

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To: microscopy-at-aaem.amc.anl.gov


} I have been considering switching to LaB6 too. What is the experience of
} Hitachi H-7000 users out there in this regard.
} -at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
} Greg Erdos Phone: 904-392-1295

We tried a Kimball Physics in our H-7000 and found it very troublesome, though
similar Kimball Lab6 emitters work well in our S-360 SEM. There was a lot of
trouble re-educating our users in using Lab6 and worst, the emission image
always had some sort of crazy-paving pattern of dark lines over it. We gave
up. We have the emitter for anyone who wants it! Hitachi claim their own
Lab6 emitters are the best (of course!) but if you deal with them at least
you could hassle your local serviceman to make it work well.






From: STEPHAN HELFER :      S.Helfer-at-rbge.org.uk
Date: Mon, 15 May 1995 09:24:04 BST
Subject: Re: immunobed sectioning

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In my experience cleaning the microscope slide thoroughly with lens
cleaning tissue removes any residues of the detergent used when
slides are washed prior to shipping. After cleaning water beads form
quite satisfactorily.

Yours sincerely


Dr Stephan Helfer, SSO
Mycologist

Royal Botanic Garden Edinburgh, Inverleith Row, EDINBURGH EH3 5LR,
Scotland UK

email s.helfer-at-rbge.org.uk
phone: +44 (0)131 552 7171 ext 280
or +44 (0)131 459 0446-280 (direct digital VoiceMail line)
fax: +44 (0)131 552 0382




From: SGKCCK
Date: 95-05-15 05:26:13 EDT
Subject: Fwd: Fixatives Shelf life

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send again
---------------------
Forwarded message:
Subj: Fixatives Shelf life

Regarding your message on your inheritance of glut and sodium cac. please
note the following:
Glut as long as it was stored away from direct sunlight and high heat can go
for years. The easiest way to test its potency is to crack open 1 ampoule
and first examine it. If it is not cloudy and does not have a percipitate
you should be in luck. To assure this take distillled water in an equal
amoun of the glut in a beaker and shake it up. It will go cloudy at first
but once it settles it should go back to being clear. If it does and there
is still no precipitate you have no problem at all.
Regarding the S.C. if it is in powder form we have found that this to lasts
for years. Make sure it has not crystallized. If it is in solution check
for preciptitate. If it has precipitated dispose of it for it is no longer
good.
I hope this helps.
Stacie Kirsch
Electron Microscopy Sciences
Fax: 215-646-8931




From: ROBIN CROSS :      EURC-at-giraffe.ru.ac.za
Date: Mon, 15 May 1995 11:46:58 GMT+0200
Subject: conference

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The 34th Annual Conference of the Electron Microscopy Society of
Southern Africa (EMSSA 95) will be held from 28 November to 1
December, 1995.

The host institution is the University of the North and the venue is
the Aventura Resort in Warmbaths, Northern Transvaal Province.

The convenors are Professor Mike Lee and Professor EM Tyobeka.

Contact address is: Prof ME Lee, EM Unit, University of the North,
Private Bag X 1106, Sovenga 0727, South Africa.

email: qemssa95-at-unin1.north.ac.za




From: SGKCCK-at-aol.com
Date: Mon, 15 May 1995 05:57:38 -0400
Subject: Fixatives shelf life

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Regarding your message on your inheritance of glut and sod. cac. please note
the following:
Glut as long as it was stored away from direct sunlight and high heat
suprisingly can go for years and be very stable. The easiest way to test its
potency is to take 10mls of the glut and firstly examine it. If there is no
precipitate and it is not cloudy the liklihood that it is good is strong. To
assure this take an equal amount of distilled water to the glut and add it
together shaking for a few seconds. The solution will go cloudy on you but
once it settles if the glut is good it will go clear again and there will be
no precipitate. This is a 100% indication that the glut is good.
Regarding sod. cac. if it is in the powder form we have found it lasts for
years. Make sure it has not crystallized. If it is in solution check
visually for precipitate. If it has precipitated which after 2 years in
solution if should have just dispose of it for it has lost its stability.
I hope this helps.
Sincerely,
Stacie Kirsch
Electron Microscopy Sciences
Fax: 215-646-8931




From: Luc Analbers :      L.J.S.Analbers-at-med.ruu.nl
Date: Mon, 15 May 1995 14:09:31 +0200
Subject: BioSoft software & intracellular [Ca2+]

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Dear confocal members,

Last week I mentioned 2 problems:

"
We would like to have a reply on 2 questions. Maybe you can help us.

1. Does anyone have measured, or knows a reference of intracellular [Ca2+] in
quiesent heart cells, preferably from neonatal rats?

2. Does anyone know software to calculate free [Ca2+] in solutions."


Dr. Kevin Pedly from London answered me. He did mention the program MAXC (by
Chris Patten) en he also mentioned the commercial software BioSoft.
I would like to know if someone is using this BioSoft program to calculate
intracellular [Ca2+]. Or maybe you know someone who is using it.
Please let me know. I do have some important questions for those persons.



Thanks.

Luc Analbers
University Utrecht
The Netherlands

Analbers-at-med.ruu.nl







From: davies-at-sol1.lrsm.upenn.edu (Peter Davies)
Date: Mon, 15 May 1995 12:58:41 -0400 (EDT)
Subject: Post-doc position Univ of Penn

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A new Postdoctoral Position will be available in the Department of
Science at the University of Pennsylvania to examine the structures,
crystal chemistry and properties of ceramic microwave dielectric
oxides.

The candidate must have experience in High Resolution TEM and a
strong backgound in Oxide Crystal Chemistry. The project will involve
structure imaging of a variety of titanate, niobate and tantalate oxide
dielectrics which are utilized as resonators in wireless communication
devices. Most of the microscopy will be conducted on the JEOL 4000EX
available in the electron microscopy facility of the Materials Research
program at Penn. The goals of the project are to correlate the
dielectric properties of these materials to changes in the cation
ordering mechansims and to utilize this information in the design and
synthesis of new oxide dielectrics.

Applicants should send their resume together with the names and telephone
numbers of three references to:

Professor Peter K. Davies; Department of Material Science & Engineering;
University of Pennsylvania; 3231 Walnut street; Philadelphia; PA19104-6272.
e-mail: DAVIES-at-SOL1.LRSM.UPENN.EDU FAX: 215-573-2128





From: Fred Hayes :      fahayes-at-ucdavis.edu
Date: Mon, 15 May 1995 10:03:23 -0700 (PDT)
Subject: IOL prep for SEM

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Does anyone out there have experience processing silicon intraocular lenses
after explant for SEM? Tommorrow (Tuesday 3/16), I am transporting a
silicon IOL from the hospital after removal from the patient. The lens
will be put into cold 2.5% Glutaraldehyde buffered in 0.1M Sodium Cacodylate
at pH 7.4. The Ophthalmologist doing the explant would like to know if
there are any inflammatory cells on the surface and what type.

Any suggestions on processing, do's and/or dont's?

Normally, I would process as follows:
1) Glut fixation for 2-3 hours
2) wash in 0.1M Sod. Caco.
3) 1% OsO4 for 30 min
4) wash in 0.1M Sod. Caco.
5) dehydrate in graded ETOH
6) 200 proof ETOH, 3 changes
7) CPD with CO2
8) mount on stub with silver paste
9) Gold coat

flat mount on the stub with either anterior or posterior surface up
depending on which surface has the most cells at the gross level.

Are there any anticipated problems with the silicon lens? ie; fracturing
from the CO2.

Thank you.

Fred A. Hayes 916-752-7712 work
University of California,Davis 916-752-4701 work
School of Medicine
Department ofMedical Pathology; EM Lab
MSIA E-mail:
Davis, CA 95616 fahayes-at-ucdavis.edu

1320 Dogwood Court 916-678-6280 home
Dixon, CA 95620-3227







From: chandler-at-lamar.ColoState.EDU (John Chandler)
Date: Mon, 15 May 1995 12:48:43 -0700
Subject: Re: IOL prep for SEM

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} Does anyone out there have experience processing silicon intraocular lenses
} after explant for SEM? Tommorrow (Tuesday 3/16), I am transporting a
} silicon IOL from the hospital after removal from the patient. The lens
} will be put into cold 2.5% Glutaraldehyde buffered in 0.1M Sodium Cacodylate
} at pH 7.4. The Ophthalmologist doing the explant would like to know if
} there are any inflammatory cells on the surface and what type.

Fred,

Most of the IOL's I have processed were polymethyl methacrylate (PMMA).
When we tried to do silicon lenses, I remember them being grossly distorted
following critical point drying. It's been several years since I have done
any of these, and I don't remember what our solution to the problem was.
I'd suggest doing some control lenses to check the effect of processing, if
that's possible. Slight distortion of the lens does not really interfere
with seeing whether there are cells on the surface. I can direct you to a
lab that has done a lot of these, if that would help. Let me know
privately. Good luck.

John
chandler-at-lamar.ColoState.EDU
http://www.vetmed.colostate.edu/anatomy/faculty/chandler.html






From: richard.easingwood-at-stonebow.otago.ac.nz (Richard Easingwood)
Date: Tue, 16 May 1995 09:07:56 +1100
Subject: Imidazole,OsO4 & alveolar fixation

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Sensitivity: Company-Confidential

I would be interested to hear from anyone who has used an imidazole
buffered osmium tetroxide fixing/staining method, particularly for alveoli.
I tried this technique with some lung tissue and found very dense, round
precipitate (or droplets), about 2um particle diameter, around the surface
of the alveoli. The surfactant within the cells was well preserved but I
would like to avoid the formation of the dense particles if possible.

Has anyone experianced this dark precipitate when using imidazole and OsO4?

Richard Lander

Richard Easingwood
South Campus Electron Microscope Unit
Otago Medical School
PO Box 913
Dunedin
NEW ZEALAND

Telephone: 64-03-479 7301
Facsimile: 64-03-479 7254

"The southernmost electron microscope unit in the world"






From: Michael Rock :      merock-at-u.washington.edu
Date: Mon, 15 May 1995 14:28:25 -0700 (PDT)
Subject: Re: IOL prep for SEM

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Fred-
we used to process intracorneal lenses, they are quite a bit different
than the silicon lens you described, but we found it important to stay
away from critical point drying. our protocol was similar to yours upto
dehydration. after going through the ethanol series we immersed the lens
in a mix 50:50 absolute ethanol & trichlorofluoroethane then a graded
series 30:70,10:90 and finally 100% trichlorofluoroethane (2x) 10 min/
solution, after the final step remove the sample and air dry (use light
flow of air, or gently shake the sample to expidite drying). mount sample,
apply conductive coating, and examine.
-Mike

On Mon, 15 May 1995, Fred Hayes wrote:

} Does anyone out there have experience processing silicon intraocular lenses
} after explant for SEM? Tommorrow (Tuesday 3/16), I am transporting a
} silicon IOL from the hospital after removal from the patient. The lens
} will be put into cold 2.5% Glutaraldehyde buffered in 0.1M Sodium Cacodylate
} at pH 7.4. The Ophthalmologist doing the explant would like to know if
} there are any inflammatory cells on the surface and what type.
}
} Any suggestions on processing, do's and/or dont's?
}
} Normally, I would process as follows:
} 1) Glut fixation for 2-3 hours
} 2) wash in 0.1M Sod. Caco.
} 3) 1% OsO4 for 30 min
} 4) wash in 0.1M Sod. Caco.
} 5) dehydrate in graded ETOH
} 6) 200 proof ETOH, 3 changes
} 7) CPD with CO2
} 8) mount on stub with silver paste
} 9) Gold coat
}
} flat mount on the stub with either anterior or posterior surface up
} depending on which surface has the most cells at the gross level.
}
} Are there any anticipated problems with the silicon lens? ie; fracturing
} from the CO2.
}
} Thank you.
}
} Fred A. Hayes 916-752-7712 work
} University of California,Davis 916-752-4701 work
} School of Medicine
} Department ofMedical Pathology; EM Lab
} MSIA E-mail:
} Davis, CA 95616 fahayes-at-ucdavis.edu
}
} 1320 Dogwood Court 916-678-6280 home
} Dixon, CA 95620-3227
}
}
}
}




From: Daniel Luchtel :      dluchtel-at-u.washington.edu
Date: Mon, 15 May 1995 15:32:03 -0700 (PDT)
Subject: Re: IOL prep for SEM

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X-Sender: dluchtel-at-homer09.u.washington.edu

I'm curious, Mike, what was the problem with critical point drying?

} we used to process intracorneal lenses, they are quite a bit different
than the silicon lens you described, but we found it important to stay
away from critical point drying. our protocol was similar to yours upto
dehydration. after going through the ethanol series we immersed the lens
in a mix 50:50 absolute ethanol & trichlorofluoroethane then a graded
series 30:70,10:90 and finally 100% trichlorofluoroethane (2x) 10 min/
solution, after the final step remove the sample and air dry (use light
flow of air, or gently shake the sample to expidite drying).




From: chandler-at-lamar.ColoState.EDU (John Chandler)
Date: Mon, 15 May 1995 17:10:56 -0700
Subject: Re: IOL prep for SEM

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Message-Id: {v01510105abdd9d9c37b5-at-[129.82.126.28]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

} I'm curious, Mike, what was the problem with critical point drying?
}
} } we used to process intracorneal lenses, they are quite a bit different
} than the silicon lens you described, but we found it important to stay
} away from critical point drying. our protocol was similar to yours upto
} dehydration. after going through the ethanol series we immersed the lens
} in a mix 50:50 absolute ethanol & trichlorofluoroethane then a graded
} series 30:70,10:90 and finally 100% trichlorofluoroethane (2x) 10 min/
} solution, after the final step remove the sample and air dry (use light
} flow of air, or gently shake the sample to expidite drying).

The problem that I remember with the silicon IOL's, from several years ago,
was that they would deform and melt at the temperatures we had in the CPD,
like 40+ deg C. Solvents seemed to be more of a problem with some of the
other lenses.

John
chandler-at-lamar.ColoState.EDU
http://www.vetmed.colostate.edu/anatomy/faculty/chandler.html






From: Stephen Schechter :      schecs-at-hopper.Sage.EDU
Date: Mon, 15 May 1995 20:00:51 -0400 (EDT)
Subject: NIOSH 7402; Asbestos Fiber ID

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Hi,

I am having a problem with the Jaffee wick washer method. After placing
the grids and filter upon the filter paper (saturated with acetone) and
letting them set for 4 to 6 hrs., the grids have fused themselves to the
underlying filter paper. Second, after examination under a dissecting
microscope, the carbon film has disintegrated. And finally, an intact
carbon film grid under the beam is unstable. Any help with these
problems will be greatly appreciated.

Thanks
Matt Kleabonas
EM Student
Stratton VAMC, Albany NY





From: GVKM07A-at-prodigy.com (DR CHARLES A GARBER)
Date: Mon, 15 May 1995 21:57:04 EDT
Subject: 3M Diampond Film Suppliers

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-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --

Scott Walck asked about suppliers of 3M diamond film for tripod
polihsing:

Try the following:

South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92672

Ph: (714) 492-2600
FAX:(714) 492-1499

e-mail (I think): 73531.1344-at-compuserve.com


Ask for Mr. David Henriks. He really knows about the various
consumable supplies that are used for tripod polishing.

Charles A. Garber
PRESIDENT
SPI SUPPLIES
PO BOX 656
WEST CHESTER, PA 19380 USA

Ph: (800) 2424-SPI
FAX:(610) 436-5755

e-mail: GVKM07A-at-prodigy.com





From: chris gilpin :      CGILPIN-at-fs2.scg.man.ac.uk
Date: Tue, 16 May 1995 9:46:56 GMT+1
Subject: etching Spurr Resin

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Hi Microscopists
I am trying to etch the surface of a Spurr resin block that has been
thick sectioned with a view to examining the exposed tissue in an SEM.
I am trynig sodium methoxide but am just getting a "gooey" surface which
obscures the tissue. The resin from the rest of the block is
dissappearing rapidly. Does anyone know of a better etchant for Spurr?

Many thanks


Chris
Chris Gilpin
Biological Sciences E.M. Unit
G452 Stopford Building
Manchester University
Oxford Road
Manchester M13 9PT
U.K.
phone 061-275-5170
fax 061-275-5171




From: Ronald LHerault :      lherault-at-acs.bu.edu
Date: Tue, 16 May 1995 08:13:09 -0400 (EDT)
Subject: silicone explant CPD

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If you can't critical point dry the object and you are imaging at 2000x
or below, have you considered makink a replica? Use polyvynil siloxane
to take an impression of the surface. It will pick up liquid droplets so
yuo should try to gently air dry the surface. Let the impression sit
overnight to out gas and then pour Spurr resin into the impression. Let
it cure, separate and mount it. Sputter coat it before viewing.

Ron





From: y. thibault :      ythibaul-at-julian.uwo.ca
Date: Tue, 16 May 1995 08:26:11 -0400 (EDT)
Subject: Na-germanate glasses analyses with EPMA

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I am planning to analyse, in the near future, Na-germanate glasses with the
electron probe microanalyzer. I am expecting that this will be somewhat
challenging and that Na will diffuse away from the beam very
significantly. I was just wondering if someone has some experience in
analyzing such glasses, and what are the best conditions and standards to
get decent results.

Thanks ,

Yves.


Yves Thibault
Dept. of Earth Sciences
University of Western Ontario
London, Ontario,
CANADA N6A 5B7





From: W.L. Steffens :      STEFFENS.B-at-calc.vet.uga.edu
Date: Tue, 16 May 1995 09:08:21 EST
Subject: Etching Spurr Resin

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To Chris Gilpin,

Sodium methoxide is a very efficient SOLVENT for just about any
polymerized epoxy resin and does not damage the tissue significantly if
osmium fixation has been carried out. The reaction must be carried out to
completion though, as any remaining partially depolymerized resin will
form the gooey residue that you see. Rather than expose the entire block
to the solvent, why not cut out the area of interest including the
sectioned face, and completely remove all remaining resin? I do this
occasionally by cutting a 3-5 micrometer section, drying it down on a
coverslip, etching away the resin, then coating and viewing it.
An alternative method might be to etch the block face in an oxygen
plasma. This of course requires a plasma asher and is more destructive to
the sample.


-=W.L. Steffens=-
College of Veterinary Medicine
University of Georgia




From: y. thibault :      ythibaul-at-julian.uwo.ca
Date: Tue, 16 May 1995 10:21:57 -0400 (EDT)
Subject: Na-germanate glasses

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I am planning to analyse, in the near future, Na-germanate glasses with the
electron probe microanalyzer. I am expecting that this will be somewhat
challenging and that Na will diffuse away from the beam very
significantly. I was just wondering if someone has some experience in
analyzing such glasses, and what are the best conditions and standards to
get decent results.

Thanks ,

Yves.


Yves Thibault
Dept. of Earth Sciences
University of Western Ontario
London, Ontario,
CANADA N6A 5B7








From: Rajesh Patel :      rpatel-at-UMDNJ.EDU
Date: Tue, 16 May 1995 13:03:32 -0400 (EDT)
Subject: Quantification of Viral particls

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=FFWPC} =02





Raj-at-Bioimg.umdnj.edu
Robert Wood Johson Medical School
Dept. of Pathology
EM Lab.
(908)235=A94648





From: dlmedli-at-california.sandia.gov (medlin douglas l)
Date: Tue, 16 May 1995 10:43:41 -0700
Subject: Re: Na-germanate glasses

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One suggestion in regards to the beam induced sodium migration is to monitor
the variation of Na signal with time in order to compensate for the
loss. Such approaches for dealing with the loss of one or more species
during microanalysis have been tried with varying degrees of success by
a number of groups.

A couple references:

Neilson and Sigurdsson, Am. Mineral. 66 (1981)
Craven, Cluckie, Duckworth and Baker, Ultramicroscopy 28 (1989) 330
Medlin and Howitt, Ultramicroscopy 48 (1993)

A couple papers specific to the sodium problem in glasses:

Miotello and Mazzoldi (1982) "Numerical analysis of field assisted sodium
migration in electron irradiated glasses," J. Phys.. C: Solid State
Physics 15, 5615-5621.

Walker and Howitt (1989) "Field induced migration of sodium in soda-silicate
glasses during scanning electron microscopy" Scanning 11, 5-11.




From: Rajesh Patel :      rpatel-at-UMDNJ.EDU
Date: Tue, 16 May 1995 15:07:54 -0400
Subject: quantificationn

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I am doing negative stain on virus particles. RThe purpose is to calculate the connoe
conc. of virus particles in a given concentration. What is the best way to
approach this?

ssorry ofor the last posting which went blank.

Raj-at-bioimg.umdnj.edu
Robert Wood Johnson Medical School
Dept. of Pathology
EM LAB
(()*)908)235-4648




From: Nancy Desmond :      nld-at-avery.med.virginia.edu
Date: Tue, 16 May 1995 16:10:10 -0400
Subject: uranyl acetate

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We are having difficulty appropriately contrasting our
conventionally prepared (aldehyde fixation in cacodylate buffer,
osmication, en bloc with uranyl acetate, epoxy embedding) brain
tissue that has traditionally been post-stained sequentially with
uranyl acetate and then lead citrate. It appears that the
culprit is the uranyl acetate. I've been told that the uranyl is
now manufactured in the former Soviet Union using a different
(i.e. safer) process. This different uranyl acetate gives
considerably less contrast in post-staining. Does anyone have
any suggestions as to other methods we might use to post-stain
formvar-coated grids? TIA,

--
Nancy L Desmond, Ph.D. nld-at-virginia.edu
Department of Neurosurgery 804.924.5607 (voice) 804.982.3829 (fax)
University of Virginia Health Sciences Center, Box 420
Charlottesville, VA 22908




From: dr.m. firtel :      temmax-at-utcc.utoronto.ca
Date: Tue, 16 May 1995 16:22:22 -0400
Subject: uranyl acetate

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unsuscribe
temmax-at-utcc.utoronto.ca




From: hukee.margaret-at-mayo.EDU (Marge Hukee)
Date: Tue, 16 May 1995 16:10:55 +0200
Subject: Re: uranyl acetate

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Message-Id: {9505162109.AA04921-at-fermat.Mayo.EDU}
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Mime-Version: 1.0
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Dear Nancy,
I too have been fighting with EMS about the quality of their uranyl, as it
is not soluble in water at a 5% concentration level. In the past I had no
trouble with this concentration. I have ordered new batch from Ted Pella
and have had the same trouble with insoluble precipitate in my 5% uranyl
even after hours of mixing. Have others had this problem, and what can we
do about it?

Marge Hukee
EM Core Facility hukee.margaret-at-mayo.edu
Mayo Foundation (507) 284-3148
----------------------------------------------------------------------------
--






From: murphy-at-ms.sjdccd.cc.ca.us (Murphy, Judy)
Date: Tue, 16 May 1995 14:32:05 PST
Subject: RE: NIOSH 7402; Asbestos Fiber ID

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Message-Id: {1995May16.143205.1954139085-at-ms.sjdccd.cc.ca.us}
To: Microscopy-at-aaem.amc.anl.gov (microscopy listserver)

Instead of filter paper, try using lens paper. Lay the lens paper over a
stainless steel bridge and be sure the edges dip into the acetone.
Sometimes the replicas get torn part because the filter paper wicks too
quickly. Of course there are lots of other things you might try, but
perhaps that will help to start with.


Judy Murphy, PhD
Dept. of Microscopy
San Joaquin Delta College
5151 Pacific Ave
Stockton, CA 95207

Phone: 209/474-5284
FAX: 209/474-5649
e-mail: murphy.ms.sjdccd.cc.ca.us







From: dr.m. firtel :      temmax-at-utcc.utoronto.ca
Date: Tue, 16 May 1995 17:36:44 -0400
Subject: unsubscribe

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unsubscribe
temmax-at-utcc.utoronto.ca




From: ggborisy-at-facstaff.wisc.edu (Gary Borisy)
Date: Tue, 16 May 1995 18:08:08 -0500
Subject: unsubscribe

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unsubscribe


______________________________________________________________________
Gary Borisy Tel: (608) 262-1365
Laboratory of Molecular Biology Fax: (608) 262-4570
University of Wisconsin-Madison Email: ggborisy-at-facstaff.wisc.edu








From: mgarment-at-facstaff.wisc.edu (Martin B. Garment)
Date: Tue, 16 May 1995 17:25:39 -0500
Subject: Fred Hayes question

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Fred,
There is a product available to keep water droplets confined on a
slide. It is called a PAP Pen, and is available from Electron Microscopy
Sciences in two sizes:
#71312 mini size, about $33
#71310 normal size, about $40
Their phone no. is 800-523-5874.
If you need any more info, please Email me directly.
I have no connection with EMS, other than as an occasional customer.





Martin B. Garment mgarment-at-facstaff.wisc.edu
Dept. of Ophthalmology (608) 262-9596 Voice
1300 University Ave. Rm 6687 (608) 262-0479 Fax
Madison, WI 53706-1532





From: Leo D Frawley 03 5667464 :      FRAWLEY-at-a1.resmel.bhp.com.au
Date: Tue, 16 May 1995 15:26:35 +1000
Subject: Sessile Drop Profile Measurement

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MR-Received: by mta VULCAN.MUAS; Relayed; Tue, 16 May 1995 15:26:35 +1000
MR-Received: by mta VULCAN; Relayed; Tue, 16 May 1995 15:26:35 +1000
Alternate-recipient: prohibited
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Has anyone ever used Mocha or any other similar image analysis software for measuring the surface tension and
contact angle of liquids from sessile drop profiles. If so, I would like to hear from you.





From: BOB ROBERTS :      ROBERTS-at-CSSS.LA.ASU.EDU
Date: Tue, 16 May 1995 17:50:31 -0700 (MST)
Subject: Unsubscribe

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Please, unsubscribe
Thank You




From: kris-at-miat0.vein.hu (Kris Kovacs)
Date: Wed, 17 May 1995 12:41:35 -0500
Subject: etching Spurr Resin

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In response to Chris Gilpin's posting "etching Spurr resin":

One technique that has been very effective is to plasma etch the cut
section using an oxygen plasma (for example, SPI Plasma Prep II, Bio-
Rad Plasma Etcher, etc). The oxygen will etch the non-osmicated
portions of the section leaving behind, in fantastic relief, the
osmicated portions. I am talking about thick sections. You can see a
micrograph demonstrating this on page 68 of the 1991 SPI Supplies
Sourcebook (e.g. catalog) of EM items. The finest details are better
preserved this way than any other way because the sample is not being
subjected to surface tension forces as the last remains of a liquid
etch (e.g. sodium methoxide) are removed.

Kris

} Hi Microscopists
} I am trying to etch the surface of a Spurr resin block that has been
} thick sectioned with a view to examining the exposed tissue in an SEM.
} I am trynig sodium methoxide but am just getting a "gooey" surface which
} obscures the tissue. The resin from the rest of the block is
} dissappearing rapidly. Does anyone know of a better etchant for Spurr?
}
} Many thanks
}
}
} Chris
} Chris Gilpin
} Biological Sciences E.M. Unit
} G452 Stopford Building
} Manchester University
} Oxford Road
} Manchester M13 9PT
} U.K.
} phone 061-275-5170
} fax 061-275-5171





From: Giles John E Jr :      giles_john_e_jr-at-space.honeywell.com
Date: 17 May 1995 07:09:07 U
Subject: EM Societies

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Hello,

Several years ago I had information concerning the Southeastern Electron
Microscopy Society and the (?) Florida Electron Microscopy Society. Does
anyone have current addresses or contact points for joining these
organizations?

Thanks,

John Giles
Senior Materials Engineer
Honeywell Space Systems M/S 225-1
13350 US Hwy 19N,
Clearwater, FL 34624
(813) 539-2270
jegiles-at-space.honeywell.com





From: SGKCCK-at-aol.com
Date: Wed, 17 May 1995 07:38:22 -0400
Subject: Uranyl Acetate

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Dear Marge,
I have seen your response to Dr. Desmond regarding your difficulties with all
of the uranyl acetate on the market currently. Please read the response that
I gave her and I would be more than happy to also give you a fresh lot for
your trial. Please let me know if this is of interest to you.
I look forward to hearing from you.
Stacie Kirsch
EMS
215-646-1566




From: SGKCCK-at-aol.com
Date: Wed, 17 May 1995 07:25:17 -0400
Subject: uranyl acetate

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Dear Dr. Desmond,
I have read your problem regarding the uranyl acetate and being able to get
acceptable contrast. Please note the following:
Back in 1993(early) the only manufacturer of depleted u.a. in Germany
discontinued the manufacture due to heavy restictions and regulations. There
was a terrible scurry to find someone else who was capable of not only
depleting the U.A. but still having the superior quality as all of us
microscopists were use to. The first few lots in the summer of 1993 that
were produced domestically down south were hideous and were over laphalized
to such an extreme that dissolving and working with a solution was nearly
impossible. This was brought to our attention late in 1993 and again the
search was on. In short since November of 1994 all of the U.A. that has been
produced is totally problem free, dissolves readily in water, and the
contrast matches any of the past good batches. We do have many microscopists
that can now attest to this. In short to rectify your problem I would
recommend highly you get a new lot which is dated either Nov 94 and on and
your problems should disappear. If by chance the U.A. you are using happens
to be ours please let us know and I will arrange for a free replacement to be
dispatched immediately. I hope this helps and I look forward to hearing from
you.
Just for your info I am not aware of U.A. being manufactured in the EX-S.U.
I look forward to hearing from you.
Stacie Kirsch
Electron Microscopy Sciences
P.O.Box 251
Fort Washington, Pa. 19034
Tel: 215-646-1566
Fax:215-646-8931




From: sje-at-po.CWRU.Edu (Steven J. Eppell)
Date: Wed, 17 May 1995 09:52:58 -0400
Subject: micro-hardness with optical microscope

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I read in an old text (1958) that there exist micro-
hardness testers that are incorporated into the
objective lens of an optical microscope. Has
anyone used one of these lenses? Would you
recommend them as a good tool for making micro-
hardness measurements? If so, what company
manufactures the lens.




From: David Henriks :      73531.1344-at-compuserve.com
Date: 17 May 95 09:58:33 EDT
Subject: Tripod Polisher User's Group Meeting

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South Bay Technology will be sponsoring a Tripod Polisher User's Group Meeting
at the MSA Conference in Kansas City.

If you have an interest in attending, please contact me off-line for complete
details.

David Henriks TEL: 800-728-2233 (toll-free in USA)
South Bay Technology, Inc. 714-492-2600
1120 Via Callejon FAX: 714-492-1499
San Clemente, CA 92673 USA e-mail: 73531.1344-at-compuserve.com





From: f705geri-at-mbox.tu-graz.ac.at (Gerald Kothleitner)
Date: Wed, 17 May 1995 17:00:42 +0200
Subject: EM film

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Message-Id: {9505171446.AA19160-at-worldlink.worldlink.com}

Hi TEM users,

since many years we have been using Ilford Technical Film EM (6.5 x 9 cm /
2 5/8 x 3 1/2 inches, 0.18 mm (7/1000 inch) polyester base) for our TEM
work.
Unfortunately Ilford is no longer providing this kind of film, so we have
to choose another type.

Is there a hidden reservoir of some more of this type of Ilford film
anywhere? (quantity, price)

Is there an EM film with similar characteristics (sensitivity, contrast,
resolution, ease of development, ...)

If you have any comments or suggestions please mail directly to

Dr Werner Grogger, FELMI, Steyrergasse 17, A-8010 Graz, Austria, Europe
e-mail: f705grog-at-mbox.tu-graz.ac.at






From: jfmjfm-at-umich.edu (John F. Mansfield)
Date: Wed, 17 May 1995 10:46:13 -0400
Subject: Meeting Announcements and Info

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New info on the World Wide Web:

Info on the 3rd Interamerican Congress on Electron Microscopy and the XV
Meeting of the Brazilian Society of EM is available at
http://www.engin.umich.edu/~jfmjfm/mas_folder/meetings.html

The 1995 MAS meeting schedule and list of abstracts is available at:
http://www.engin.umich.edu/~jfmjfm/mas_folder/breck.html

Remember that there is a MAS home page and also a Michigan ELectron
Microscopy Society Home Page. These can be found by checking out:
http://www.engin.umich.edu/~jfmjfm/mseandemalmain.html

Let me know if there are problems.

John Mansfield.


John Mansfield
North Campus Electron Microbeam Analysis Laboratory
413 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (313)936-3352 FAX (313)936-3352
jfmjfm-at-engin.umich.edu, John.F.Mansfield-at-umich.edu
or jfmjfm-at-umich.edu they all reach me!
URL: http://www.engin.umich.edu/~jfmjfm/jfmjfm.html






From: Bob Keller
Date: 5/17/95 2:50 PM
Subject: Re: TEM- high speed video

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"Microscopy" {Microscopy-at-aaem.amc.anl.gov}
X-Mailer: Mail*Link SMTP/QM 3.0.0

Reply to: RE} TEM: high speed video

Bob,
This is very hard to do with most systems as the yag is already attached
to a normal TV directly making changing the camera itself problematic. The
HVEM uses a lens coupled system and therefore could be converted given a
suitable camera.
This however brings up some new problems. Most image intensifiers, as
well as the yag itself have persistance that would cause you problems. This is
compounded by the lower light levels caused by the higher frame speeds.
Doug Owen, NCEM
--------------------------------------

EM centers have such systems that guests could use. What would be a practical
limit on frames/sec? I presume it's not limited by the electron flux. The
interest centers on dislocation velocities in several metals.

Please post responses to the listserver.

Thank you.

Bob Keller
NIST
Materials Reliability Division
Boulder, CO

Bob





From: Michael OKeefe :      Michael_OKeefe-at-macmail7.lbl.gov
Date: 17 May 1995 16:41:01 U
Subject: Re: TEM- high speed video

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Message-Id: {n1411393337.57793-at-macmail7.lbl.gov}

Reply to: RE} TEM: high speed video

Bob Keller wrote -

} As part of some in-situ straining experiments, we would like to obtain
} results from a high speed video system (MUCH greater than 30 frames/
} sec is desired).
} I'd like to ask what capabilities exist, and whether any of the national
} (U.S.) EM centers have such systems that guests could use. What would
} be a practical limit on frames/sec? I presume it's not limited by the
} electron flux. The interest centers on dislocation velocities in several
} metals.

} Please post responses to the listserver.

-------------------------------------------------
Bob,
at the NCEM we are set up to run in-situ straining (with or w/o heating) at
energies up to 1.5MeV in our Kratos EM-1500. We use an intensified TV camera
at 30fps. We looked at hi-speed systems and concluded that the 128x128 Kodak
CCD that runs at up to 40,000fps using 64 output channels would be suitable.
However we were not sure that we would have sufficient intensity in our YAG
scintillator.
Mike O'Keefe, NCEM





From: STEPHAN HELFER :      S.Helfer-at-rbge.org.uk
Date: Wed, 17 May 1995 14:11:45 BST
Subject: Microscopical Societies

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To: microscopy-at-aaem.amc.anl.gov

Earlier today John Giles wrote:

Hello,

Several years ago I had information concerning the Southeastern
Electron
Microscopy Society and the (?) Florida Electron Microscopy Society.
Does
anyone have current addresses or contact points for joining these
organizations?

Thanks,

John Giles

This prompted me to ask for information on regional Microscopical
Societies and their activities.
I suggest that there should be a 'register' of such groups to enable
users to get intouch when they need a local forum. If people could
send me their information I would be willing to keep this list for
anyone to see. I would like the following format: 'Name of group';
'scope'; 'geographical cover' (local, national, international); 'principal
events'; 'contact person'.
I suppose I may as well start the ball rolling:
There are five Microscopy groups active in Scotland (Aberdeen,
Dundee, Edinburgh, Glasgow and St. Andrews)
Edinburgh Microscopical Society; all microscopic disciplines; local
South East Scotland; members' meetings (ca 4 per year), annual
Scottish Microscopy Symposium (together with other Scottish groups);
Stephan Helfer membership secretary s.helfer-at-rbge.org.uk.

Yours sincerely


Dr Stephan Helfer, SSO
Mycologist

Royal Botanic Garden Edinburgh, Inverleith Row, EDINBURGH EH3 5LR,
Scotland UK

email s.helfer-at-rbge.org.uk
phone: +44 (0)131 552 7171 ext 280
or +44 (0)131 459 0446-280 (direct digital VoiceMail line)
fax: +44 (0)131 552 0382




From: David Henriks :      73531.1344-at-compuserve.com
Date: 17 May 95 09:59:02 EDT
Subject: Workshop on Tripod Polishing for TEM

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WORKSHOP ON TRIPOD POLISHING

WORKSHOP OBJECTIVE
This course will cover all aspects of TEM pre-thinning and focus on final
thinning via Tripod Polishing and ion milling. Due to the limited class size
and the extensive hands-on opportunities, this course is well suited to novices
as well as advanced Tripodders. The course will include sections on:

How to do it and why should I?
What's really going on and what am I really seeing?
How to prepare small, specifc area cross-sections.
The problem of wildy differing materials (eg tungsten).
Rapid preparation of TEM cross-sections.
Preparation of a wide range of amterials: semiconductors, ceramics, metals,....

HANDS-ON OPPORTUNITY
This course will be unique in that it will provide a hands-on opportunity for
every participant. Tripod Polishers, Polishing Wheels, and pre-thinning
equipment will be made available to participants and actual samples will be
prepared - by the students - as part of the course. This is a great opportunity
to get your hands dirty and learn by doing. The instructors will walk you
through each step of the process and then let you loose on the equipment.

WORKSHOP LOCATION AND DATES
South Bay Technology, Inc. - San Clemente, CA
Dates: July 21-22, 1995

INSTRUCTOR
Ron Anderson
IBM
East Fishkill Facility
Hopewell Junction, NY

CLASS SIZE
Due to the intensive hands-on aspects of this course, class sisze will be
strictly limited to 10 participants.

REGISTRATION FEE: $495 (Includes lunches and Friday night dinner)
REGISTRATION DEADLINE: June 15, 1995

For additional information or to register for the workshop, please contact:

David Henriks TEL: 800-728-2233 (toll-free in USA)
South Bay Technology, Inc. 714-492-2600
1120 Via Callejon FAX: 714-492-1499
San Clemente, CA 92673 USA e-mail: 73531.1344-at-compuserve.com





From: Joseph P. Neilly 708-938-5024 :      NEILLY.JOSEPH-at-igate.abbott.com
Date: Wed, 17 May 1995 08:00:00 -0600 (CST)
Subject: TEM Calibration

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Mr-Received: by mta RANDB; Relayed; Wed, 17 May 1995 09:07:08 -0600
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Folks,

We are currently in the process of writing a standard operating
procedure (SOP) for measuring the actual magnification of our 2 TEMs
(Philips CM12 and Philips EM201). Our approach is a fairly standard
one. We plan to take pictures of a replica grating or catalase crystals
(depending on the mag) measuring the spacings on the standards from the
negatives and calculating the actual magnification. We have a question
about the procedure we thought we would ask the group.

How many measurements do people take per magnification? Is one enough
or should we take several measurements from a single negative or several
measurements from several negatives at the same magnification. Part of
our group feels multiple measurements should average out any errors in
the standard or the measuring process. Others feel (and have some data
to suggest) the multiple measurements does not increase the accuracy
significantly and would be wasted time and effort. What is the
consensus of people who have done this procedure?

Thanks in advance for any replies.

Joe Neilly
Department of Cellular and Microscopic Research
Abbott Laboratories
Abbott Park, IL 60064
Phone: 708-398-5024
Email: Neilly.joseph-at-igate.abbott.com






From: Marija Gajdardziska Josisovska :      mgj-at-csd.uwm.edu
Date: Wed, 17 May 1995 16:08:26 -0500
Subject: TEM Textbooks

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Dear fellows microscopists and professors:

Can we start a discussion on BEST GRADUATE LEVEL TEXTBOOKS FOR
PHYSICAL AND BIOLOGICAL MICROSCOPY?

I am establishing a new HREM laboratory at the Physics Dept. at the University
of Wisconsin Milwaukee. In the Fall 1995 semester I will teach a new graduate
course in TEM (* see course description). Selecting a required textbook is my
present problem. Please share your textbook choices with me.

Sincerely,
Marija Gajdardziska-Josifovska
Assistant Professor,
Department of Physics, UWM
P.O. Box 413
Milwaukee, WI 53201
Tel. (414) 229 4965

********************************************************************************
*PHY 904 Electron Microscopy, 3 Credits, Graduate

Interactions of high energy electrons with solids will be described, as
encountered in transmission electron microscopes. Scattering by ordered
periodic objects (crystals) and disordered structures (defects, amorphous
materials) will be covered, along with the imaging, diffraction and
spectroscopy methods of microscopy.
Emphasis will be given to microscopy of solid surfaces,interfaces and
small particles, which are at the crux of research in the Laboratory
for Surface Studies. The course will include:

¥ *kinematical and dynamical theory of diffraction (scattering factors,
multislice formulation of Schroedinger's equation);
¥ *experimental diffraction modes (selected area diffraction, convergent
beam diffraction, nanodiffraction, reflection high energy electron diffraction);
¥ *transfer function imaging theory (weak phase object approximation,
linear and nonlinear imaging, wavefront reconstruction);
*transmission and reflection imaging modes (bright field, dark field, high
resolution electron microscopy, reflection electron microscopy, electron
holography);
¥ *basic spectroscopy modes (energy dispersive X-ray spectroscopy,
electron energy loss spectroscopy).

The course will be required from students who plan to incorporate electron
microscopy in their Ph.D. projects. It is designed for graduate students in
physics, chemistry and materials science. Advanced students in biology may
also take the course as a sequence to 542. Prerequisite is an undergraduate
solid state course (i.e. level of Kittel).




From: C.Veitch-at-geel.dwt.csiro.au (Colin Veitch)
Date: Wed, 17 May 1995 16:08:26 -0500
Subject: Micromanipulators

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To: microscopy-at-aaem.amc.anl.gov

Hi all,

We wish to install micromanipulators on an Hitachi S4100 FESEM. Does anyone
know of a company which manufactures/sells them and if they have an agent in
the Antipodes? (or at least in Australia!!)

Thanks in advance.

Colin Veitch
###############################################################################
# #
# Colin J. Veitch C.Veitch-at-geel.dwt.csiro.au #
# CSIRO Division of Wool Technology Tel. +61 (0) 52 275611 #
# P.O. Box 21 Fax. +61 (0) 52 275657 #
# BELMONT Vic 3216 #
# Australia #
# #
# "We see the Universe the way it is because if it were different, we would #
# not be here to observe it." #
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From: Arthur Gillman :      ARGIL-at-delphi.com
Date: Wed, 17 May 1995 21:59:23 -0400 (EDT)
Subject: Microhardness tester

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Steven Eppell wrote:
} I read in an old text (1958) that there exist micro-
} hardness testers that are incorporated into the
} objective lens of an optical microscope. Has
} anyone used one of these lenses? Would you
} recommend them as a good tool for making micro-
} hardness measurements? If so, what company
} manufactures the lens.

This method is still used, although there are a lot of variants. In one,
a diamond indenter is used to put a diamond shaped indentation in the
sample. The size is then measured by a microscope and video setup, and
related to hardness. My company used to make a video measurement system
that not only read the size of the indentation, but also read out the
hardness in appropriate units. The device was marketed by Olympus, but not
too many were sold. We may still have one.

We still make video measurement systems, but not the hardness tester.
There are a number of people making hardness testers which work on the same
principle. I don't know if the combination indenter/objectives are still
being used. I think Wilson and others had systems like that.

Arthur Gillman
Princeton, NJ




From: Barbara Reine :      reine-at-u.washington.edu
Date: Wed, 17 May 1995 18:41:17 -0700 (PDT)
Subject: More Info Re: 3rd IACEM Meeting

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Third Interamerican Congress on Electron Microscopy

and

XV Meeting of the Brazilian Society for Electron Microscopy

2-6 September, 1995
HOTEL GLORIA
Caxambu, Minas Gerais, Brazil


The 3rd Interamerican Congress on Electron Microscopy (IACEM) will be
held jointly with the XV Meeting of the Brazilian Society of Electron
Microscopy (BSEM), at the Hotel Gloria in Caxambu, Brazil, September 2nd
to September 6th, 1995. The program will cover fundamentals and
applications of electron microscopical techniques in the fields of
Biological and Materials Science. Invited talks and workshops will
highlight current topics in both fields. Contributed papers on current
and unpublished results will be selected for poster and oral
presentations. Accepted abstracts will be published as a supplement of
Acta Microscopica.

Official languages are Portuguese, English and Spanish.

Caxambu is a pleasant town known for its mineral spring water. It is
located at 900 m above sea level, where local temperatures in September
range from 12 deg. C at night to 28 deg. C during the day. Caxambu can be
easily reached from Rio de Janeiro, Sao Paulo, or Belo Horizonte by
commercial bus service.


INVITED SPEAKERS

R. Albrecht (USA) Correlative HRM
M. Burgos (Argentina) EM History in Latin America
M.G. Burke (USA) Defect Analysis in Super Alloys
R. Dallai (Italy) Insect Sperm Ultrastructure
M. Ellisman (USA) 3-D Reconstruction
M.A. Goldstein (USA) 3-D Reconstruction of Proteins
N. Hirokawa (Japan) Cytochemistry
G. L'Esperance (Canada) Quantitaive AEM
Y. Ishida (Japan) HREM Interfaces
B. Jouffrey (France) EELS Spectroscopy
W. Massover (USA) Protein Molecules
A. Maunsbach (Denmark) EM of Kidney Epithelium
M. McCartney (USA) Electron Holography
B.F. McEwen (USA) Mitosis Mechanisms
M. Muller (Switzerland) Cryo Techniques
S. Miyazawa (Japan) Japan's Biomedical State of the Art
A.B. Noguera (Venezuela) Immunolabeling
A. Ourmazd (USA) STM, HREM, Semi-conductors
R. Padron (Venezuela) Thick Filaments Structure
F. Ponce (USA) Atomic Arrangements, GaN Epitaxy
R. Sinclair (USA) Magnetic Materials, "In situ" HREM
R. Singer (USA) "In situ" hybridization
J. Slot (Netherlands) Cryomethods
D. Williams (USA) Analytical Electron Microscopy
K. Zierold (Germany) Cryo-Ultramicotomy


REGISTRATION FEES

BSEM & IFSEM Members
Professional US$ 50.00 (until 6/20) US$ 70.00 (after 6/20)
Student US$ 25.00 (until 6/20) US$ 35.00 (after 6/20)

Non-Members
Professional US$100.00 (until 6/20) US$120.00 (after 6/20)
Student US$ 35.00 (until 6/20) US$ 45.00 (after 6/20)

The registration fee includes a copy of the book of abstracts
and participation in a Welcome Reception.


CALL FOR ABSTRACTS: JUNE 20th DEADLINE

Please submit abstracts as follows:
1. The title is centered and in upper case letters.
2. The names of the authors and their affiliations are centered and
typed in upper and lower case letters. Include the full adress of
the authors to be contacted.
3. Abstracts must be submitted in camera-ready form within a single
17 x 23 cm page.
Text: 17 cm wide x 12 cm long, single column.
Illustrations: 17 cm wide x 10 cm long.

IMPORTANT: A person may figure as the senior author in only one
abstract. Senior authors or one of the co-authors must register.

4. Use a laser or laser quality printer.
5. Abstracts may be submitted in Portuguese, English or Spanish.
6. Send the original with one copy and the completed registration form
to the Organizing Committee.
7. Please do not send abstracts by fax or E-mail.
8. Abstracts will be selected by an editorial committee and a
notification of acceptance will be mailed to the authors.
9. DEADLINE FOR RECEIPT OF ABSTRACTS IS JUNE 20, 1995 (mailing date).

For a model abstract form, contact Elliot Kitajima or Barbara Reine
(addresses, etc. listed at the end of this announcement).


POSTERS

The space reserved for each poster is 1.0 m wide x 1.2 m long. Posters
may be attached to the formica surface with scotch tape.


MEETING SCHEDULE

September 2, 1995
9am-3pm Registration
5pm Opening Ceremony
7:30pm Reception and Diner

September 3-6

9am-12pm Symposia
2pm-6pm Invited lectures and
instrument development presentations.
8pm-11pm Poster sessions

September 6

12pm Closing ceremony


TRANSPORTATION

The official airline for the Congress is VARIG - Brazilian Airlines. It
is advised to book your flight in advance.

Caxambu can be easily reached from Rio de Janeiro, Sao Paulo or Belo
Horizonte by commercial bus service, leaving from bus stations. The trip
takes 5-7 hours and is priced around US$10.00. The bus companies
operating these lines are:

From Rio de Janeiro: Cidade do Aco - buses leave daily at 7am and 8pm.

From Belo Horizonte: Expresso Gardenia - buses leave daily at 7:30am & 11pm.

From Sao Paulo: Rezendense - buses leave daily at 8am and 10:15pm.

Efforts will be made to provide special buses departing from
Rio de Janeiro and Sao Paulo for Congress participants.


ACCOMMODATIONS

The Hotel Gloria has special rates for Congress participants.
Rates* per person are listed below.

New section Old section
single US$ 75.00 US$ 45.00
double US$ 42.00 US$ 29.00

*Meals included in all rates.

The rooms in the hotel's older section are not equipped with TV,
refrigerator or telephone. Rooms shared by more than 2 persons (limit of
6) have lower rates. For reservations contact the Hotel Gloria by
phone/fax: (55-35)3415001/(55-35)3411552.


Other hotels within walking distance:

Palace (55-35) 3411040
single or double room US$ 50.00
room for 4 US$ 90.00*

Lopez (55-35) 3411120
single or double room US$ 56.00
room for 3 US$ 81.00
room for 4 US$ 106.00
room for 5 US$ 131.00*

Brazil (55-35) 3411203
per person US$ 20.00
(breakfast only)

*Meals included.


COMMERCIAL EXHIBITS

The Congress offers excellent opportunities for commercial companies to
exhibit their products in a very convenient area adjoining the major
meeting rooms and poster sessions.

Commercial exhibitors should contact the Organizing Committee before June
20th for stand reservation and advertisement in the proceedings book.





___________________________________________________________________________

REGISTRATION FORM

Name:

Complete Address:



Area of interest: __ Biological Sciences __ Material Sciences

Status: __ Professional __ Student

Member of BSEM or IFSEM: __ Yes __ No

Poster presentation: __ Yes __ No

If yes: Title:

Authors:

Note: Registration fee must be included in the submission of the
abstract. Checks must be payable to Sociedade Brasileira de Microscopia
Eletronica.

Please return the registration form, abstract and registration fee to:

E.W. Kitajima, 3rd IACEM and XV MBSEM
Dept. Biol. Cel. - IB, Univ. Brasilia
70919-970 Brasilia, DF, Brazil




_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

Please fill in the 2nd part of the form:

Name:

Address:


We acknowledge the receipt of US$ (registration fee)

check # bank #

In case of abstract submission:
Title:

Authors:

Abstract: __ Accepted __ Not Accepted




ADDITIONAL INFORMATION

For additional information please contact:

3rd IACEM and XV MBSEM
E.W. Kitajima
Dept. Biologia Celular - IB
Universidade de Bras70919-970
BrasTel: (55-61)3482424
Fax: (55-61)3499094 or 2741065
E-mail: kitajima-at-guarany.cpd.unb.br

or

Barbara Reine
University of Washington
Botany Dept. Box 351330
Seattle, WA 98195-1330
Tel:(206)543-1955
Fax:(206)543-4413
E-mail: reine-at-u.washington.edu














From: jfmjfm-at-umich.edu (John F. Mansfield)
Date: Thu, 18 May 1995 10:05:23 -0400
Subject: Re: Microscopical Societies

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I suggest that we have Web Pages describing the various local societies and
thir charters and officers. These could be linked to from a central web
page on the MSA and MAS web sites. If people want to send me the relevant
informtion. I will set this up.

To do this properly I would want a uniform set of information from each society.
1. Society name and area.
2. Affiliation (MAS, MSA, MSC, etc) i.e. this should be world wide.
2. Current Officers names.
3. Society Charter
4. Meetings, when, where, how frequent, etc.
5. Publications.

This is a FREE offer folks, take advantage of it!

} Earlier today John Giles wrote:
}
} Hello,
}
} Several years ago I had information concerning the Southeastern
} Electron
} Microscopy Society and the (?) Florida Electron Microscopy Society.
} Does
} anyone have current addresses or contact points for joining these
} organizations?
}
} Thanks,
}
} John Giles
}
} This prompted me to ask for information on regional Microscopical
} Societies and their activities.
} I suggest that there should be a 'register' of such groups to enable
} users to get intouch when they need a local forum. If people could
} send me their information I would be willing to keep this list for
} anyone to see. I would like the following format: 'Name of group';
} 'scope'; 'geographical cover' (local, national, international); 'principal
} events'; 'contact person'.
} I suppose I may as well start the ball rolling:
} There are five Microscopy groups active in Scotland (Aberdeen,
} Dundee, Edinburgh, Glasgow and St. Andrews)
} Edinburgh Microscopical Society; all microscopic disciplines; local
} South East Scotland; members' meetings (ca 4 per year), annual
} Scottish Microscopy Symposium (together with other Scottish groups);
} Stephan Helfer membership secretary s.helfer-at-rbge.org.uk.
}
} Yours sincerely
}
}
} Dr Stephan Helfer, SSO
} Mycologist
}
} Royal Botanic Garden Edinburgh, Inverleith Row, EDINBURGH EH3 5LR,
} Scotland UK
}
} email s.helfer-at-rbge.org.uk
} phone: +44 (0)131 552 7171 ext 280
} or +44 (0)131 459 0446-280 (direct digital VoiceMail line)
} fax: +44 (0)131 552 0382

John Mansfield
North Campus Electron Microbeam Analysis Laboratory
413 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (313)936-3352 FAX (313)936-3352
jfmjfm-at-engin.umich.edu, John.F.Mansfield-at-umich.edu
or jfmjfm-at-umich.edu they all reach me!
URL: http://www.engin.umich.edu/~jfmjfm/jfmjfm.html






From: lmiller-at-ux1.cso.uiuc.edu (Lou Ann Miller)
Date: Thu, 18 May 1995 09:53:23 -0600
Subject: CSMS Meeting, Road Closed

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Message-Id: {199505181449.AA02691-at-ux1.cso.uiuc.edu}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Greetings!


For those attending the Central States Microscopy Meeting, there are some
direction changes on how to get there.

The map sent out indicates a road called "St Mary's Road".
Usually this is the prime choice road to get to our facility.

However, the University has dug a deep tunnel across it to put a steam
tunnel in.
The rains have flooded the road as well.
(} 3.5 inches in 2 hours, more rain on the way. Another building close by
had its auditorium under water and mud for up to the 1st 2 rows of seats
above stage level!)

So: Take Kirby Avenue or Windsor Road to reach Lincoln Ave where we are.

Thanks!!!

Lou Ann

***********************
Lou Ann Miller
Microscopic Imaging Laboratory
College of Veterinary Medicine
University of Illinois
2001 S Lincoln Ave
Urbana, Illinois 61801
217-244-1566
lmiller-at-ux1.cso.uiuc.edu
***********************






From: Michael Cammer :      cammer-at-aecom.yu.edu
Date: Thu, 18 May 1995 12:18:14 -0400 (EDT)
Subject: the naming of facilities is a difficult matter...

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Would those associated with facilities please send me (personally at
cammer-at-aecom.yu.edu) the name of your facility.
We are considering renaming our EM and other microscopy facilities and
would like an idea of what names other groups/schools are using. We also
do image analysis.
Thanks-
Michael Cammer






From: rutledge phil :      prutle1-at-gl.umbc.edu
Date: Fri, 19 May 1995 11:38:56 -0400
Subject: retina fixation

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I am currently doing TEM on the retinas of cuttle fish and was wondering
if anyone would know of a good fix for this type of specimen. I tried
2.5% glut in filtered sea water but the fixation doesn't look so good.
If anyone has a decent protocol for the fixation and processing of theses
type of retinas, I would appreciate your faxing or e-mailing a copy to me.

Thanks in advance,

Phil Rutledge

voice: (410) 455-3582
fax: (410) 455-3875
e-mail: prutle1-at-gl.umbc.edu




From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 18 May 1995 15:46:38 -0400
Subject: RE-MAC Abs Jmp Ratios

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Subject: Time: 3:34 PM
OFFICE MEMO RE:MAC Abs Jmp Ratios Date: 5/18/95

I believe the usual defininton of the "absorption edge jump ratio" is that
used by Heinrich in his classical article "X-Ray Absorption Uncertainty" that
was published (p. 296 - 377) in THE ELECTRON MICROPROBE, John Wiley & Sons,
1966 (ISBN No. 65-26849); that is, it is the value of the mass absorption
coefficient on the 'high' side of an absorption edge divided by the value of
the MAC on the low side of the edge. This matter is discussed beginning on
p. 330 of this reference. Figures 18 and 21, and Table X give values for
jump ratios for selected K, L, and M edges. If you can't find this
reference, I could FAX a copy of the few pages relating to this topic to you
if you send me your FAX number.





From: dlmedli-at-california.sandia.gov (medlin douglas l)
Date: Thu, 18 May 1995 13:02:47 -0700
Subject: Re: TEM Textbooks

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Reimer's book, "Transmission Electron Microscopy--Physics of Image Formation
and Microanalysis" Springer Series in Optical Sciences, Volume 36 (Springer-
Verlag, 1989), is a nice comprehensive overview with a large number of re
references.

+---------------------------------------------+
! Douglas L. Medlin !
! Physical Properties of Materials Department !
! Mail Stop 9402 !
! Sandia National Laboratories !
! Livermore, California 94551 !
! !
! (510) 294-2825 !
! dlmedli-at-california.sandia.gov !
+---------------------------------------------+




From: masur-at-msvax.mssm.edu
Date: Fri, 19 May 1995 14:36:29 -0500
Subject: trade axiomat for smaller scope

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is anyone interested in trading or buying from us an inverted axiomat
(equipted for fluorescence, nomarski and phase (plus air table)?

we need similar optics but in a more compact scope (inverted or upright
would do)


Sandra K. Masur Box 1183
fax: 212-289-5945 Mount Sinai School of Medicine
phone: 212-241-6544 or 0089 NY NY 10029-6574
e-mail:masur-at-msvax.mssm.edu









From: FRANCISCO J HERNANDEZ BLAZQUEZ fzea - zab 0195 616122 - 283 :      fjhblazq-at-usp.br
Date: Fri, 19 May 1995 16:45:36 -0500 (CDT)
Subject: historesin sections with ondulations

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bioforum-at-net.bio.net

I am with a problem when I cut historesin sections
Sometimes the sections are "vibrated", the plastic
is ondulated and if the section is stained, its
aspect is "striated".
Why this occurs?

Thanks for any help.
=============================================================================
Francisco Javier Hernandez Blazquez |
Universidade de Sao Paulo - Faculdade de | e-mail fjhblazq-at-usp.br
Zootecnia e Engenharia de Alimentos | Voice: 55 195 616122 r. 265
Departamento de Ciencias Basicas/Histologia| r. 278
Av. Duque de Caxias Norte, 225 CP 23 | Fax: 55 195 611689
CEP 13630-000 Pirassununga (Sao Paulo) | 55 11 8694831
BRAZIL |
==============================================================================





From: em-at-mediacity.com (E. Monberg)
Date: Fri, 19 May 1995 17:32:44 -0800
Subject: EM vs OM

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Message-Id: {v01510101abe2f5353940-at-[192.0.2.1]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Dear Nestor's list and Nestor,

Since 99% of your posts relate to EM,

how about a separate group for Optical

Microscopy - I know, the last advances

came in 1898 - but a few vendors are coming

along with nice "data flow segregated mode"

tricks like confocals AND I'd like to buy

a used instrument from time to time.

(psst. - if any list menmbers are selling,

please let me know)


Thanks,

Ed Monberg
LMDC






From: Beverly E Maleeff :      Beverly_E_Maleeff%notes-at-sb.com
Date: 19 May 95 17:38:27 EDT
Subject: re:the naming of facilities is a difficult matter...

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Message-Id: {9505200023.AA0379-at-pho018.sb.com}
To: microscopy {microscopy-at-aaem.amc.anl.gov}

Would those associated with facilities please send me (personally at
cammer-at-aecom.yu.edu) the name of your facility.
We are considering renaming our EM and other microscopy facilities and
would like an idea of what names other groups/schools are using. We also
do image analysis.
Thanks-
Michael Cammer

The MSA Technologists' Forum, and specifically Sandy Silvers, has compiled an
EM Facilities Directory. You may be able to get the information you're looking
for from that publication. Sandy's phone number is 706/546-3471.

Bev Maleeff
SmithKline Beecham Pharmaceuticals
Toxicology-US, UE0462
709 Swedeland Road
King of Prussia, PA 19406
610/270-7987
610/270-7202 fax
maleeffbe-at-sb.com





From: Laurie Frederick :      frederick_laurie-at-vanlab.paprican.ca
Date: Thu, 18 May 1995 15:25:03 PDT
Subject: EDX source list for beginners

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Hi,
As promised some time ago, I have compiled a "brief" list of EDX
information and equipment sources intended to assist someone
starting out in the field. It is six pages long, available in Wordperfect
6.0 format and includes the following headings:
1) Organizations
2) Books and Periodicals
3) Conferances
4) Courses
5) Internet Resources
6) EDX systems - Sun Sparcstation Based systems
- PC, Mac or Power Mac Based systems
- "Up-grade" systems (economy approach)
7) X-ray standard manufacturers (and their Canadian rep.'s)
8) Carbon Evaporator Manufacturers
9) Worthwhile Accessories
10) Digital Imaging References

This is not a complete list, I will continue to add to it over time for my
own benefit and it undoubtly has a Canadian bias but I will be happy to
send an e-mail copy (as an attached file) to anyone who requests it.
Have a good day.
Laurie

______________________________________________________________________
Laurie Frederick, A.SC.T. PAPRICAN
Corrosion Control Group 3800 Wesbrook Mall
The Pulp and Paper Research Vancouver, B.C.
Institute of Canada Canada V6S 2L9

Email: frederick_laurie-at-vanlab.paprican.ca
Tel: 604-222-3200 Fax: 604-222-3207




From: James Wesley-Smith :      WESLEYSM-at-biology.und.ac.za
Date: Fri, 19 May 1995 09:49:19 +0200 (SAST)
Subject: RE: Uranyl acetate staining

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Message-ID: {MAILQUEUE-101.950519094919.173-at-biology.und.ac.za}

Dear colleagues
I have been following the comments/ suggestions regarding Uac
staining. I would like to refer anyone interested to an
excellent article by Peter R. Lewis, The Mechanisms of Positive
Staining, published in the Proceeding of the Royal Microscopical
Society (Vol. 22/6, Nov 1987). He deals with the fundamentals of
staining, and suggests the most likely reactions taking place during
staining with heavy metal stains. Insofar as U ac is concerned he
describes uranyl acetate as an ionic stain which is bound by
phosphate groups of DNA and RNA. He suggestes the solutions of U ac
contain a "bewildering variety of molecules, each with differing
reactivity". He describes these solutions as unstable, leading to
varying staining results with time (perhaps addressing the FAQs "does
U ac go off in storage?"). In this respect pH plays a central role,
which should be kept below pH5. Hayat (1970) suggests that lower pH
(3.5) and increasing dilution results in an increase in staining
intensity and specificity of nucleic acids.

I can fax this article to anyone interested. Please contact me
directly. As a closing comment, shouldn't we go back to basics before
blaming the russians?

James Wesley-Smith
EM Unit
Unibversity of Natal
Durban, South Africa




From: Laurie Frederick :      frederick_laurie-at-vanlab.paprican.ca
Date: Thu, 18 May 1995 09:36:27 PDT
Subject: Re: micromanipulators

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Alternate-Recipient: allowed
Auto-Forwarded: prohibited
Content-Return: allowed
Disclose-Recipients: prohibited
Conversion: allowed
Importance: normal
Priority: normal
Sensitivity: Company-Confidential
Microscopy ListServer {MICROSCOPY-at-aaem.amc.anl.gov}
Message-Id: {950518182713.589-at-cliff.ml.wpafb.af.mil.0}

Hi,
I don't know what the particles consist of but I examined inclusions
from a Nickel alloy once by disolving the metal with 35% Bromine in
methanol under a Nitrogen atmosphere. The liquids were transfered
and filtered thru micron filters with the aid of vacuum. The inclusions
such as TiN and NbC etc. were examined in an SEM on the filter paper
but it would have been simple enough to transfer them to a TEM grid.
Just a thought, it might be a different approach.
Laurie
}
} Dear friends &colleagues,
}
}
} Jordi and I are working on a project that requires us to examine by
TEM
} micron size particles in a metal matrix. We would like to remove these
} individual particles and place them on a grid. We have heard about a
tool
} called a micromanipulator yet no one we work with has ever used
one before.
} If any one has used one before for any purpose, or could give us
some
} information about them just for our general knowledge, it would be
greatly
} appreciated.
}
}
} Thank you,
} Andrea Monisera 1-201-455-4922
}
}
}
}
}

______________________________________________________________________
Laurie Frederick, A.SC.T. PAPRICAN
Corrosion Control Group 3800 Wesbrook Mall
The Pulp and Paper Research Vancouver, B.C.
Institute of Canada Canada V6S 2L9

Email: frederick_laurie-at-vanlab.paprican.ca
Tel: 604-222-3200 Fax: 604-222-3207




From: Nancy K. Smith :      SMITHN%A1%SHIRE-at-mr.uthscsa.edu
Date: Fri, 19 May 1995 11:24:12 -0500 (CDT)
Subject: 'NKRS'

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--Boundary 007c78a000990963
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--Boundary 007c78a000990963
Content-type: MESSAGE/RFC822

To: ANLEMC.MSD.ANL.GOV


RFI:
Does anyone out there know anything about the commercial availability of an
instrument known as a laser feedback microscope? My source is Science, Sept 3
1993, p 1275. It reports that the instrument was "introduced" at the MSA meeting
in Cincinnati by Berkeley biophysicist Alan Bearden. It is supposed to be great
at measuring precise heights along the surface of a sample. The dept of
prosthodontics at our dental school is looking for such capability & has $ to
spend.
Thanks, nkrs

--Boundary 007c78a000990963
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==============================================================================|
| | |
| Nancy K.R. Smith, Ph.D. | Work phone: 210-567-3861 |
| Associate Professor | Work fax: 210-567-3803 |
| Dept. of Cellular & Structural Biology| Home phone: 210-690-0687 |
| University of Texas Health Science | Home fax: 210-690-0687 |
| Center at San Antonio | (Call & tell us to turn on the |
| 7703 Floyd Curl Drive | fax machine) |
| San Antonio TX 78284-7762 | |
| | |
===============================================================================


--Boundary 007c78a000990963--




From: nina allen :      allen-at-wfu.edu
Date: Fri, 19 May 1995 23:38:34 -0400 (EDT)
Subject: Re: the naming of facilities is a difficult matter...

Contents Retrieved from Microscopy Listserver Archives
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microscopy-at-aaem.amc.anl.gov



I am setting up a new facility at NC State University. we named it CMIC
or Cellular and Molecular Imaging Center.
Nina S. Allen.

On Thu, 18 May 1995, Michael
Cammer wrote:

} Would those associated with facilities please send me (personally at
} cammer-at-aecom.yu.edu) the name of your facility.
} We are considering renaming our EM and other microscopy facilities and
} would like an idea of what names other groups/schools are using. We also
} do image analysis.
} Thanks-
} Michael Cammer
}
}




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sat, 20 May 1995 7:30:14 -0500 (CDT)
Subject: Meetings on Microscopy

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Alternate-Recipient: allowed
Auto-Forwarded: prohibited
Content-Return: allowed
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Importance: normal
Priority: normal
Sensitivity: Company-Confidential
Microscopy ListServer {MICROSCOPY-at-aaem.amc.anl.gov}
Message-Id: {950518182945.589-at-cliff.ml.wpafb.af.mil.0}


John McCaffrey asks for future meetings postings.

If you have access to the WWW the Microscopy and Microanalysis WWW Site
which I also maintain has a listing of all meetings I hear about. Currently
the list has 19 Microscopy/Microanalysis meetings listed for the rest of 1995
and about 6 for 1996.


Use any WWW browsing program and supply the URL (address) of

http://www.amc.anl.gov

If you wish to submit information for inclusion the instructions are
posted at the end of the meetings list.

Nestor
Your Friendly Neighborhood SysOp.


P.S.

Short Course offerings are listed seperately.




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sat, 20 May 1995 7:41:12 -0500 (CDT)
Subject: LIst of meeting from the WWW Site

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Oops... It occurs to me that some of you may not
have access to the WWW yet. So I've done a simple
cut and paste of the WWW site meeting list. Please
note that some special characters will have become
garbled slightly and the formatting is not
as nice as it is on the WWW site. In addition, hypertext links to
further information will not show up on this posting. This
list covers meeting that I know about from May of 1995 - 1996.

Cheers... Nestor

================



¥ 30th annual meeting of the SouthEastern Microscopy Society (SEMS)

May 17-19, 1995

Omni Hotel in Atlanta, Ga, USA

Contact:Janet H. Woodward, SEMS '95 Program Chair

Tel: +1 912-945-3152

Fax: +1 912-945-3155

¥ Central States Microscopy Meeting

May 19,1995

University of Illinois , College of Veterinary Medicine in Champaign-Urbana

Contact:Lou Ann Miller, Microscopic Imaging Laboratory, College of Veterinary
Medicine, University of Illinois Rm 1215 Basic Science Bld, 2001 S Lincoln Ave,
Urbana, Illinois 61801

Tel:+1 217-244-1566 (7am-4pm CST)

Fax:+1 217-333-4628

E-mail: lmiller-at-ux1.cso.uiuc.edu

¥ Midwest Society of Electron Microscopy Workshop

June 2-3, 1995

Topic:Computers and Microscopy

Fredrick Center, Madison, Wisconsin USA

Contact: Grayson Scott, Univ. of Wisconsin,Electron Microscope
Facility,Madison,
Wisconsin 53706

Tel: +1 608-262-2993

Fax: +1 608-262-7306

Email:glscott-at-facstaff.wisc.edu

¥ 22nd Annual Meeting of the Microscopical Society of Canada

June, 4-7, 1995

University of Ottawa, Canada

Contact: Jim Corbett, Department of Physics, University of Waterloo, Waterloo,
Ontario, Canada, N2L 3G1

Tel. +1519 885 1211

Fax +1519 746 8115

E-mail corbett-at-physics.watstar.uwaterloo.ca

¥ 3rd Annual Atomic Force/Scanning Tunneling Microscopy Symposium

June 6-9, 1995

Natick, MA, USA

Contact: Samuel H Cohen, Science and Technology Directorate, US Army Natick
RD&E Center, Natick, MA 01760-5020, USA

Tel. +1 508 651-4478

Fax +1 508 651-5104

E-mail dcobban-at-natick-emhl.army.mil

¥ Protocols in: Image Analysis and Confocal and Electron Microscopy

June, 7-9, 1995

Washington D.C.

Contact:F.G. Lightfoot, The George Washington University Center for Microscopy
and Image Analysis, Suite 406, 2300 I St. NW. Washington D.C. 20037

Tel: +1 202 994-2881

Fax: +1 202 994 8885

Email:FredL-at-INDY.CMIA.GWUMC.EDU

¥ American Association of Feed Microscopists

June 19-23, 1995

San Antonio, Texas

Topic: Annual Meeting 19-20, Short Course 21-23

Sponsor: American Association of Feed Microscopists

Contact: Marjorie McCutcheon, P. O. Box 5246, Charleston, West Virginia 25361

Tel: 304-558-2208

Fax: 304-558-3594

¥ Trinocular Joint Meeting on Electron Microscopies

June 26-30, 1995

Lausanne, Switzerland

Sponsors: Socit Franaise de Microscopie Electronique, Socit Belge de
Microscopie/Belgische Vereniging Voor Microscopie Socit Suisse d'Optique et de
Microscopie Electronique/Schweizerische Gesellschft f¥r Optik und
Electronenmikroskopie

Contact:CongrÏs Trinoculaire

Centre Interdpartemental de Microscopie Electronique

EPFL, CH - 1015 Lausanne, Suisse

¥ MicroBeam Analysis Society Annual Meeting

Aug. 6-11, 1995

BreckenRidge, Colorado, USA

Contact: Edgar S. Etz, Bldg. 222/A113,National Institute of Standards and
Technology,Gaithersburg, MD 20899

Tel: +1 301 975-3909

Fax: +1 301 216-1134

E-Mail: etz-at-gapnet.nist.gov

¥ Microscopy Society of America/HistoChemical Society(Joint Annual Meeting)

Aug 14-18, 1995

Kansas City,Mo

Contact: MSA Business Office

Tel:+1 508-540-5594 / 800-538-3672

Fax:+1 508-548-9053

Email: mmaser-at-mbl.edu

¥ 3rd Inter-American Congress on Electron Microscopy

Sept. 2-6, 1995

Caxambu,MG, Brazil

Contact: Elliot Watanabe Kitajima, Departamento de Biologia Celular,
Universidade de Brasilia, 70919-970 Brasilia, DF, Brazil

Tel. +55-61-348-2424/+55-61-340-9094

Fax +55-61-274-1065

E-mail kitajima-at-guarany.cpd.unb.br

¥ New Zealand Microscopy (EM and LM) Conference

September 4th-8th 1995

Dunedin, New Zealand

Contact: Allan Mitchell, C/-Department of Anatomy and Structure, Otago Medical
School, PO Box 913, Dunedin, New Zealand

Tel: (+64) 3 479 7301

Fax: (+64) 3 479 7254

email: allan.mitchell-at-stonebow.otago.ac.nz

¥ EMAG 95

Sept. 12-15, 1995

University of Birmingham, UK

Contact: Institute of Physics, EMAG 95, 47 Belgrave Square, London SW1X 8QX, UK

Tel. +44 171 235 6111

Fax +44 171 823 1051

E-mail iopconf-at-ulcc.ac.uk

¥ 14th. Annual Advances in Microscopy Symposium

Sept. 29 - Oct. 1, 1995

Wrightsville Beach, North Carolina

Topic:Microscopy Outreach-Conveying its Science, Art and Technology

Sponsor: North Carolina Society for Microscopy and Microbeam Analysis

Contact: Peter Ingram, Ann LeFurgey, Box 3709, Duke University, Medical Center,
DURHAM NC 27710

Tel: (919) 684-3534

Fax: (919) 681-8419

E-mail: ingram-at-rti.org

¥ First Annual Symposium on Integrated Microscopy

Sept. 29 - Oct. 1, 1995

University of Wisconsin-Madison

Topic: Combined Microscopies in Biological Problems

Sponsor: Integrated Microscopy Resource, University of Wisconsin-Madison


Contact: IMR, University of Wisconsin-Madison, 1675 Observatory Drive, Madison,
WI 53706

Email: imradmin-at-calshp.cals.wisc.edu

¥ 23rd Scottish Microscopy Symposium

November 15, 1995

Sponsor:Edinburgh Microscopical Society

MacRobert Pavilion, Royal Highland Centre, Ingliston, Edinburgh EH28 8NF,
Scotland

Chairperson: Dr Martin Maxwell

Secretary: Dr Ciara Clarke

Membership: Dr Stephan Helfer

Telephone: 0131 552-7171

Voice Mail: 0131 459 0446-280

FAX: 0131 552-0382

email: stephan-at-rbge.org.uk

¥ International Seminar on Quantitative Microscopy

Oct. 4-5, 1995

Braunschweig, Germany

Contact: Physikalische- Technische Bundesanstalt, Seminar on Quantitative
Microscopy, H. Geuther, Lab. 4.22, Postfach 3345, D-38023 Braunschweig, Germany

Fax: +49 531 592 4015

E-mail: heinrich.geuther-at-ptb.de

Meetings in 1996
¥ 14th Australian Conference on Electron Microscopy

Feb 5-9, 1996

University of Sydney, Australia

Contact: ACEM14 - microCOSMOPOLITAN, E.M.Unit, University of Sydney, NSW 2006,
Australia

Tel: 61 2 351 2351

Fax: 61 2 552 1967

¥ Micro 96

2-4 July, 1996

London, UK

Contact: The Royal Microscopical Society, 37/38 St. Clements, Oxford OX4 1AJ,
UK

Tel: +44 1865 248768

Fax: +44 1865 791237

¥ 6th Asia-Pacific Conference on Electron Microscopy, APEM 6

August 1996

Hong Kong

Contact: Dr. EC Chew, Department of Anatomy, University of Hong Kong, Shatin,
New Territories, Hong Kong

Tel: +852 609 6845

Fax: +852 603 5031

¥ 17th Congress and General Assembly of the International Union for
Crystallography

8-17 August,1996

Seattle, USA

Contact: Prof. RF Bryan, Department of Chemistry, University of Virginia,
Charlottesville VA 22903, USA

¥ Microscopy Society of America/MicroBeam Analysis Society/Microscopial
Society
of Canada(Joint Annual Meeting)

Aug 11-15, 1996

Minneapolis, MN, USA

Contact: MSA Business Office

Tel:+1 508-540-5594 / 800-538-3672

Fax:+1 508-548-9053

Email: mmaser-at-mbl.edu

¥ EUREM 96

26-30 August,1996

University College Dublin, Ireland

Contact: Prof. Martin Steer, EUREM 96 Office, Botany Department, University
College Dublin, Belfield, Dublin 4, Ireland

Tel: +353 1 7062254

Fax: +353 1 7061153

Know of a meeting that should be added to this page?

Contact Nestor J. Zaluzec (EMail: Zaluzec-at-aaem.amc.anl.gov)

I will gladly add the hypertext link.

Please provide the following information.
¥ Meeting Name
¥ Meeting Dates
¥ Meeting Topic or Short Description
¥ Meeting Sponsor (Society/Organization/University)
¥ Contact Person
¥ Full Postal Address
¥ Telephone Number
¥ Fax Number
¥ Email Address
¥ URL to a WWW information page if available





From: Mr Herbert Mohr :      H.Mohr-at-ph.surrey.ac.uk
Date: Sat, 20 May 1995 16:01:06 +0100
Subject: unsubscribe php1hm@pierre.ph.surrey.ac.uk

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unsubscribe




From: Chris Jefferies :      chris-at-stowey.demon.co.uk
Date: Sat, 20 May 1995 11:43:45 GMT
Subject: Re: Notice of upcoming microscopy meetings

Contents Retrieved from Microscopy Listserver Archives
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In his message dated Thursday 18, May 1995, John McCaffrey wrote :

} Hello!
} I'm not sure how everyone's budget planning goes, but for ours we need
} to put in our budgets in March for the entire fiscal year (Mar. 31, 19xy to
} Mar. 31, 19(xy+1). If we don't know about an interesting upcoming meeting,
} we can't budget for it and hence cannot attend. I'd like to request that
} conference planners let us all know as soon as possible of upcoming meetings,
} so we don't miss out because we hadn't planned for it.

Good point I think. I know that budgeting in the UK is along very similar
annual lines. Anyone with access to the World Wide Web should keep an eye on my
meetings page at {URL:http://metro.turnpike.net/jefferie/meetings.html} .

I add every meeting I hear about, it currently runs right through to 1997! If
it's announced on this list it'll almost certainly make it to my page.

Hope this helps,

Chris
--
---------------------------------------------------------------------------
| Chris Jefferies E-Mail at home - chris-at-stowey.demon.co.uk |
| at work - chris.jefferies-at-bbsrc.ac.uk |
| Microscopy page {URL: http://metro.turnpike.net/jefferie/index.html} |
---------------------------------------------------------------------------




From: c4647-at-SLVAXA.UMSL.EDU (Phil Fraundorf)
Date: Sun, 21 May 1995 07:47:51 -0500
Subject: HREM/SPM - CONSTRUCTING low-vibration labspace

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Hi,

The opportunity to prepare space from the ground up for high resolution
electron (and scanned probe) microscopy is relatively rare. This note is
just to ask for ideas and contacts, as well as more success & horror
stories, on this subject.

Specifically, architects have designed several rooms in the bottom floor
of a new 3-story building to be sound and vibrationally isolated from the
rest of the building by: (i) placing the floor of those rooms on a separate
more massive foundation resting on 30-foot columns, and (ii) routing
building services (e.g. air handling, plumbing, electricity) away from those
areas. This is in an environment which tests low for vibration already.
They are now beginning the construction phase, and I would feel better if I
can propose some intermediate phase tests to ensure that indeed something
has not been overlooked. I am, of course, also interested in the experience
of others in this regard, and in suggestions as to who might have relevant
experiences to relate.

Building construction is not quite like buying a microscope: If it
doesn't work, you probably will still have to live with it. As success is
likely to involve some serendipity as well as diligence, I thought this
server might be one place to look.

Cheers. /philf :)

//\/\/\/\--}
// P.Fraundorf c4647-at-slvaxa.umsl.edu http://newton.umsl.edu/pfhomepg
\\ Physics & Astronomy, U. Missouri-St.Louis MO 63121 USA 314-516-5044
\\/\/\/\/\/\/\/--}





From: Campbell36-at-aol.com
Date: Sun, 21 May 1995 12:01:46 -0400
Subject: Re: LIst of meeting from the WWW Site

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


What is the URL for the microscopy WWW site?

Thanks
Jim Campbell




From: Kittel Agnes :      kittel-at-kokiux.koki.hu
Date: Mon, 22 May 1995 11:25:59 +0200 (MET DST)
Subject: Re: triple labelling of antigens

Contents Retrieved from Microscopy Listserver Archives
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Please unscribe




From: Sally Shrom :      sally-at-retina.anatomy.upenn.edu
Date: Mon, 22 May 1995 09:32:02 -0400 (EDT)
Subject: EPON problems?Please help!

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Posted-Date: Mon, 22 May 1995 09:32:36 -0400

I have been doing immunocytochemistry for the last 5 years and have never
had problems with epon infiltration or polymerization. ( we stain with
DAB) Now 2 times in a row I have bad tissue. I can't pinpoint the
problem. The tissue (cat retina) looks muddy and shrunken. The epon
itself doesn't even section as well as it should- it looks rippled and
compressed. I am doing everything the same here except I opened new
bottles of resins. Is it possible to get a bad batch of resins from a
reputable company? If you can help, please respond to my E-mail address:
sally-at-retina.anatomy.upenn.edu




From: Joyce Craig :      bafpjec-at-uxa.ecn.bgu.edu
Date: Mon, 22 May 1995 10:47:57 -0500 (CDT)
Subject: Re: HREM/SPM - CONSTRUCTING low-vibration labspace

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone know a simple method for checking vibration?




From: A. Kent Christensen :      akc-at-umich.edu
Date: Mon, 22 May 1995 12:07:26 -0400 (EDT)
Subject: Re: EM vs OM

Contents Retrieved from Microscopy Listserver Archives
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Additional comment on the message from Ed Monberg. I think that the
name of this list conference, "Microscopy", is well chosen, embracing both
EM and LM. I would hate to see them separated, since most of us (at least
in biological microscopy) deal with both. Anyone witnessing the current
"California gold rush" in confocal microscopy is well aware that LM still
has great vigor and inovation. I tend to speak of "light microscopy" (LM)
rather than "optical microscopy" (OM), since one can buy books on electron
optics, suggesting that EM is also optical.

-----------------------------------




From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 22 May 1995 12:25:12 -0400
Subject: RE-CheckingVibs&Fields

Contents Retrieved from Microscopy Listserver Archives
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Message-ID: {n1410976896.34867-at-mse.engin.umich.edu}

Subject: Time: 12:19 PM
OFFICE MEMO RE:CheckingVibs&Fields Date: 5/22/95

There is a company that we have used that specializes in measuring mechanical
vibrations and magnetic fields in instrumentaion laboratories. I can't find
their name and address right now, but our EM Lab Manager, George Brooks will
be able to give it to you: gbrooks-at-umich.edu.





From: Elinor Solit :      cambrex-at-world.std.com
Date: Mon, 22 May 1995 12:12:29 +0059 (EDT)
Subject: Re: Phosphor Powder

Contents Retrieved from Microscopy Listserver Archives
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Lucille,

Try Galileo in western Massachusetts( are codes 413 or 508). You can
probably contact them through JEOL, which sells their SEM product or call me by phone for
their number. I don't have it right at hand.

Hope this helps.


Ellie Solit
The Microscope Book
617-742-0311

On Mon, 22 May 1995, Lucille A. Giannuzzi wrote:

} Does anyone know where I can get some Phospher powder for coating screens?
}
} Thanks,
}
} Lucille Giannuzzi
}
}
} *************************************************************************
} Lucille A. Giannuzzi, Ph.D.
}
} Dept. of Mechanical and Aerospace Eng. phone (407) 823-5770
} University of Central Florida fax (407) 823-0208
} 4000 Central Florida Blvd. email lag-at-pegasus.cc.ucf.edu
} Orlando, FL 32816-2450 USA
} *************************************************************************
}
}
}




From: Jay Jerome :      jjerome-at-isnet.is.wfu.edu
Date: Mon, 22 May 1995 12:12:48 -0400 (EDT)
Subject: Re: EM vs OM

Contents Retrieved from Microscopy Listserver Archives
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I disagree. I began many years ago as a photon microscopist then began
using EM more as it suited my needs. Lately, however, with many new
optical techniques Photon Microscopy is having a resurgence. Correlative
microscopy (OM and EM together) is particularly useful. I predict much
more of us will be using OM in the near future. Thats my HO.

best-

Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-isnet.is.wfu.edu *
**************************************************************

On Mon, 22 May 1995, Azriel Gorski wrote:

} }
} } Dear Nestor's list and Nestor,
} }
} } Since 99% of your posts relate to EM,
} }
} } how about a separate group for Optical
} }
} } Microscopy -
}
} I too would like to see this, but I am afraid that in the chase after "new
} technology", we who use classical microscopy to chase the humble photon are
} becoming a distinct majority.
}
} } I know, the last advances
} }
} } came in 1898 - but a few vendors are coming
} }
}
} Sorry, but the above stuck in my craw. The list of advances is long since that
} date. Thanks (am I saying this?) to computers we now have much better objectives
} and what about infinite image distance objectives?
}
} } along with nice "data flow segregated mode"
} }
} } tricks like confocals AND I'd like to buy
} }
} } a used instrument from time to time.
} }
} } (psst. - if any list menmbers are selling,
} }
} } please let me know)
} }
} }
} } Thanks,
} }
} } Ed Monberg
} } LMDC
} }
}
} Shalom from Jerusalem,
} Azriel Gorski
} +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
} Azriel Gorski, Head gorski-at-vms.huji.ac.il
} Optical Microscopy Laboratory
} Israel National Police, Jerusalem
} TEL: 972-2-309437
} FAX: 972-2-309360 (Attn: A. Gorski)
}
} Opinions expressed here are the author's alone. They in no way represent the
} opinions, positions or policies of the Israel National Police.
}
}




From: M.V. Parthasarathy :      mvp2-at-cornell.edu
Date: Mon, 22 May 1995 15:09:18 -0400 (EDT)
Subject: Checking for Vibration

Contents Retrieved from Microscopy Listserver Archives
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X-NUPop-Charset: English

A company called Vibration Engineering Consultants, inc., (VEC) Oakland, CA
specializes in conducting vibration and EMI surveys. Contact Wayne Vogen at
Tel: 510 339-8719; FAX: 510 339-2352.

*************************************************************************
M.V. Parthasarathy
Professor of Plant Biology & Director, EM Facility
Section of Plant Biology
228 Plant Science Building
Cornell University, Ithaca, NY 14850
Telephone: (607) 255-1734
Fax: (607) 255-5407
E-mail: mvp2-at-cornell.edu
***********************************************************************




From: Jay Jerome :      jjerome-at-isnet.is.wfu.edu
Date: Mon, 22 May 1995 17:05:14 -0400 (EDT)
Subject: Re: EM Societies

Contents Retrieved from Microscopy Listserver Archives
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Information about and applications to join the

SOUTHEASTERN MICROSCOPY SOCIETY (SEMS) can be obtained from:

Beth Richardson, University of GA,
beth-at-dogwood.botany.uga.edu


Information about and applications to join the

APPALACHIAN REGIONAL ELECTRON MICROSCOPY SOCIETY (AREMS)
can be obtained from:

Stephanie Evans, Bowman Gray Sch. Med., stevans-at-isnet.is.wfu.edu


I hope this information is helpful-
best-

Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-isnet.is.wfu.edu *
**************************************************************

On 17 May 1995, Giles John E Jr wrote:

} Hello,
}
} Several years ago I had information concerning the Southeastern Electron
} Microscopy Society and the (?) Florida Electron Microscopy Society. Does
} anyone have current addresses or contact points for joining these
} organizations?
}
} Thanks,
}
} John Giles
} Senior Materials Engineer
} Honeywell Space Systems M/S 225-1
} 13350 US Hwy 19N,
} Clearwater, FL 34624
} (813) 539-2270
} jegiles-at-space.honeywell.com
}
}




From: Fred Hayes :      fahayes-at-ucdavis.edu
Date: Mon, 22 May 1995 14:57:53 -0700 (PDT)
Subject: Re: EPON problems?Please help!

Contents Retrieved from Microscopy Listserver Archives
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On Mon, 22 May 1995, Sally Shrom wrote:

} I have been doing immunocytochemistry for the last 5 years and have never
} had problems with epon infiltration or polymerization. ( we stain with
} DAB) Now 2 times in a row I have bad tissue. I can't pinpoint the
} problem. The tissue (cat retina) looks muddy and shrunken. The epon
} itself doesn't even section as well as it should- it looks rippled and
} compressed. I am doing everything the same here except I opened new
} bottles of resins. Is it possible to get a bad batch of resins from a
} reputable company? If you can help, please respond to my E-mail address:
} sally-at-retina.anatomy.upenn.edu
}
Sally,

1) yes it's possible to get a bad batch of resins, but unlikely.
Polymerize some blank blocks as a control.

2) having sections which are compressed and are rippling is a sure sign
of incomplete infiltration and/or uneven or incomplete polymerization.
Try putting your blocks back into the oven.

3) how much experience do you have embedding cat retina? Does the retina
have attached choroid/sclera/muscle? I embedded cat retinas for four
years exclusively with Spurr's and recommend it highly. Epon will work,
just make sure it's infiltrated very well. Did you use an intermediate
solvent between dehydration and embedding? You could have residual
solvent left in the tissue. Is the block soft? Thats usually the cause of
compression. Perhaps the formula of the resin was incorrect. The muddy
appearance may be the Tapetum which underlies the retina. Is this an
observation at the gross, LM or EM level?











Fred A. Hayes 916-752-7712 work
University of California,Davis 916-752-4701 work
School of Medicine
Department ofMedical Pathology; EM Lab
MSIA E-mail:
Davis, CA 95616 fahayes-at-ucdavis.edu

1320 Dogwood Court 916-678-6280 home
Dixon, CA 95620-3227







From: Fred Hayes :      fahayes-at-ucdavis.edu
Date: Mon, 22 May 1995 16:44:43 -0700 (PDT)
Subject: Re: historesin sections with ondulations

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Jose Roberto Machado Cunha da Silva {jrmscdsil-at-spider.usp.br} ,
bioforum-at-net.bio.net

On Fri, 19 May 1995, FRANCISCO J HERNANDEZ BLAZQUEZ fzea - zab 0195 616122 - 283 wrote:

} I am with a problem when I cut historesin sections
} Sometimes the sections are "vibrated", the plastic
} is ondulated and if the section is stained, its
} aspect is "striated".
} Why this occurs?
}
} Thanks for any help.
} =============================================================================
} Francisco Javier Hernandez Blazquez |
} Universidade de Sao Paulo - Faculdade de | e-mail fjhblazq-at-usp.br
} Zootecnia e Engenharia de Alimentos | Voice: 55 195 616122 r. 265
} Departamento de Ciencias Basicas/Histologia| r. 278
} Av. Duque de Caxias Norte, 225 CP 23 | Fax: 55 195 611689
} CEP 13630-000 Pirassununga (Sao Paulo) | 55 11 8694831
} BRAZIL |
} ==============================================================================
}
}

Francisco,

the undulations or vibrations is compression produced by all or some of:

a) dull glass knife and/or a bad angle (less than 4 degrees or greater
than 6)

b) bad left right alignment of knife edge to block face

c) wrong cutting speed

d) too thick or too thin of a section (3-5 microns is normal)

e) bad block face orientation to the knife edge

f) block face may be too big with bad dimensions (depends on your choice
of microtome [ histo vs ultra ] and size of glass knife. Long axis
of the block face should be verticle to the knife edge.

g) manual cut is always better than auto. You have better control

h) polymerization may be either incomplete or too soft of a formula or both

i) tissue may be incorrectly orientated on the long axis to the knife edge

j) tissue may not be completely infiltrated or polymerized


***histo resins are soft in nature, and are less complicated in formula
than epoxies. If you follow the manufacturers recipe, you should have
no problem. 9 out of 10 times it's a sectioning parameter. Good luck


Fred A. Hayes 916-752-7712 work
University of California,Davis 916-752-4701 work
School of Medicine
Department ofMedical Pathology; EM Lab
MSIA E-mail:
Davis, CA 95616 fahayes-at-ucdavis.edu

1320 Dogwood Court 916-678-6280 home
Dixon, CA 95620-3227







From: m.dickson-at-unsw.edu.au (Melvyn R. Dickson)
Date: Tue, 23 May 1995 11:49:15
Subject: Re: HREM/SPM - CONSTRUCTING low-vibration labspace

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To: microscopy-at-aaem.amc.anl.gov

In article {Pine.3.89.9505221008.A10102-0100000-at-ecom1.ecn.bgu.edu} Joyce Craig {bafpjec-at-uxa.ecn.bgu.edu} writes:
} Date: Mon, 22 May 1995 10:47:57 -0500 (CDT)
} From: Joyce Craig {bafpjec-at-uxa.ecn.bgu.edu}
} Subject: Re: HREM/SPM - CONSTRUCTING low-vibration labspace

} Does anyone know a simple method for checking vibration?

A quick and cheap way is to put a petri dish of water (or mercury if youre
courageous) on the floor, bench, whatever you need to test. Look at the
reflection of a ceiling light on the surface. If there are ripples, you have
too much vibration.




From: FRANCISCO J HERNANDEZ BLAZQUEZ fzea - zab 0195 616122 - 283 :      fjhblazq-at-usp.br
Date: Mon, 22 May 1995 23:32:44 -0500 (CDT)
Subject: Re: historesin sections with ondulations

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Jose Roberto Machado Cunha da Silva {jrmscdsil-at-spider.usp.br} ,
bioforum-at-net.bio.net

Dr. Hayes

I'M very grateful for your help. The problem was loose blocks and
the "chatter" disappeared.

Thank you

=============================================================================
Francisco Javier Hernandez Blazquez |
Universidade de Sao Paulo - Faculdade de | e-mail fjhblazq-at-usp.br
Zootecnia e Engenharia de Alimentos | Voice: 55 195 616122 r. 265
Departamento de Ciencias Basicas/Histologia| r. 278
Av. Duque de Caxias Norte, 225 CP 23 | Fax: 55 195 611689
CEP 13630-000 Pirassununga (Sao Paulo) | 55 11 8694831
BRAZIL |
==============================================================================





From: Dirk Knoesen, UWC, SA :      DIRK-at-physics.uwc.ac.za
Date: 23 May 95 09:49:44 GMT+0200
Subject: Re: Buildings, Vibrations for TEM

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Message-ID: {MAILQUEUE-101.950523094944.320-at-physics.uwc.ac.za}
To: microscopy-at-aaem.amc.anl.gov

Dear Phil Fraundorff
We have been in the process of designing and building a new EM
facility for our faculty during the last few years, and can give the
following info that we have checked for and design in to the EM rooms.
The EM rooms are a separate building with its own main power and
ground, not connected to the main building. Each room has a
reinforced concrete slab about 1 m thick (3 ft) for the floor,
isolated from the walls, resting on a sand bottom. The power supply
to all light fittings and power points is wired NOT in serial but
from the main power (to prevent possible ground loops or even worse,
when the power is supplied from a loop around the room). The rooms
are run on slight over pressure from the air conditioning (to reduce
dust, etc). The following checks were done on the site before and
after the completion of the building:
1. Vibration checks: use a small mirror on the floor, reflect a
laser beam off it on the ceiling. It is sensitive enough to pick up
the vibrations of footsteps on the floor.
2. Magnetic, electrical: Initially we used a coil from an old
demonstration transformer with 10000 turns, and connect it to a
oscilloscope. Our main problem was the main power cable to the
whole building that ran about 50 yrs away next to a univ road, but
its influence was small enough to be within the limits given by the
TEM manufacturer.
Maybe I should mention that the main EM we uses is a 200 kV Hitachi
H800 TEM, although we do not use it for high-resolution work.

Success with the building
Dirk Knoesen

Prof Dirk Knoesen U U W W CCCCCC
Department of Physics U U W W C
University of the Western Cape U U W W W C
Private Bag X17, Bellville 7535 U U W W W W C
South Africa UUUUUU W W CCCCCC
Tel:+21 959 2266. Fax:+21 959 3474. Internet: dirk-at-physics.uwc.ac.za




From: mk-at-enk.ks.se (Martin K|hler)
Date: Tue, 23 May 1995 13:00:47 +0200
Subject: Re: EM vs OM

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I AGREE with Ed!

Why not separate the microscopy list into one LM list and
one list containing "the rest" (EM,AFM et.c.)?

People interested in both could of cource subscribe both lists!!

This would solve my, and probably others, problem of too
many mails that has to be sorted out.

/ Martin


Ed wrote:
} Dear Nestor's list and Nestor,
} Since 99% of your posts relate to EM,
} how about a separate group for Optical
} Microscopy - I know, the last advances
} came in 1898 - but a few vendors are coming
} along with nice "data flow segregated mode"
} tricks like confocals AND I'd like to buy
} a used instrument from time to time.
} (psst. - if any list menmbers are selling,
} please let me know)
}
} Thanks,
}
} Ed Monberg
} LMDC








From: Ubirajara Pereira Rodrigues Filho :      ubira-at-iqm.unicamp.br
Date: Tue, 23 May 1995 09:18:30 -0300 (GMT-0300)
Subject: TEM-cellulose acetate porous membranes preparations

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Dear Net's;
Can someone help me? I'm working with cellulose acetate porous
membranes coated with a thin film of zirconium dioxide. I was studying
this membranes with SEM X-ray microanalysis system to study the
distribution of the oxide on the membrane surfaces. This task was
succesfull, but when we try see the grain boundary we can't. Then we
solve use the TEM for this pourpose. We embebed the membrane in a epoxi
resin to cut in a ultramicrotome, but the membrane structure is collapsed
and we lost the porous structure.
Thank you in advance,

Ubirajara Pereira Rodrigues Filho
Instituto de Quimica- UNICAMP
Campinas, SP, Brazil, 13083-970, CP 6154
e-mail: ubira-at-iqm.unicamp.br




From: Jay Jerome :      jjerome-at-isnet.is.wfu.edu
Date: Tue, 23 May 1995 09:56:41 -0400 (EDT)
Subject: Re: EM vs OM

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In every list I have ever become a member of there comes a point when
postings become large and a group then wants to split off. The offshoot,
except for a few, is that everyone else joins all of the subbranches.
Informattion is cross posted to all branches and your mail goes sky high.
I am also on the confocal microscopy list and much of the information
there is cross posted here.
Is there really a serious need to initiate an optical microscopy list? I
for one would rather see this list evolve into one which can encompass
the needs of the Photon Microscopist.
A too full mail box will always be a problem. If a light microscope list
is set up it will become popular. Do you then subdivide it into
fluorescence, video, Nomarski, etc??????
In my humble opinion good subject lines and a quick delete finger are the
only real solutions to the "too much e-mail" problem.

Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-isnet.is.wfu.edu *
**************************************************************

On Tue, 23 May 1995, Martin K|hler wrote:

}
} I AGREE with Ed!
}
} Why not separate the microscopy list into one LM list and
} one list containing "the rest" (EM,AFM et.c.)?
}
} People interested in both could of cource subscribe both lists!!
}
} This would solve my, and probably others, problem of too
} many mails that has to be sorted out.
}
} / Martin
}
}
} Ed wrote:
} } Dear Nestor's list and Nestor,
} } Since 99% of your posts relate to EM,
} } how about a separate group for Optical
} } Microscopy - I know, the last advances
} } came in 1898 - but a few vendors are coming
} } along with nice "data flow segregated mode"
} } tricks like confocals AND I'd like to buy
} } a used instrument from time to time.
} } (psst. - if any list menmbers are selling,
} } please let me know)
} }
} } Thanks,
} }
} } Ed Monberg
} } LMDC
}
}
}
}
}




From: kris-at-miat0.vein.hu (Kris Kovacs)
Date: Tue, 23 May 1995 16:00:40 -0500
Subject: Re: EM vs OM

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Dear Fellow Microscopists:
(including LM, EM, AFM, ETC...)

My comment to Martin's and Ed's reflections is definitely NO. And again NO.
I completely disagree with them.

The international trend is to reunite again the people. The Committee of
European Societies for Electron Microscopy (CESEM) has changed it's name to
Committee of European Societies for Microscopy (CESM). The Hungarian Society
for Electron Microscopy changed it's name to Hungarian Society for
Microscopy. Although this is a small society of slightly more than two
hundred members the same happens to other societies, not to mention the USA.
We (I mean electron microscopists) routinely use light microscopes. And
although the same is not necessarily valid to all light microscopist I am
convinced the flow of ideas might be beneficial to all other people. For
example I used to read almost all messages, even the Life Science related
ones, although I'm a materials scientist. As for me it is always easier to
select messages of real interest out of one single list than to check one
more list. I don't want to provoke a lengthy discussion on this subject but
shall we really move against international trends?

Hope some of you will agree with me.

One more point: in our society we don't even separate Life Science and
Materials Science people. On our yearly general assembly we used to have
scientific sessions for two days, and all lectures are presented to
everybody. These cover topics of general interest, and believe me: the
discussion between people working on quite different fields is really
fertilizing.

Kris
(President of the Hungarian Society for Microscopy)

Dr. Kristof Kovacs
Central Laboratory
University of Veszprem
H-8201 Veszprem, P.O.Box 158, HUNGARY
Phone: +36-(88)-421-684
Fax: +36-(88)-426-01
Dr. Kristof KOVACS
University of Veszprem, Central Laboratory
P.O.Box 158, Veszprem, HUNGARY
H-8201
Phone: +36-(88)-421-684
Fax: +36-(88)-426-016





From: Michael OKeefe :      Michael_OKeefe-at-macmail7.lbl.gov
Date: 23 May 1995 09:45:56 U
Subject: Re: EM vs OM

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Reply to: RE} } EM vs OM

Hey, great idea!
Then we can separate out EM and AFM, the biologists and
the materials scientists, then the transmission and
scanning people, then the drivers of Japanese microscopes
from those who prefer European microscopes,
then the Republicans and the Democrats,
then we can all have our own private lists!
Hmm, maybe it's not such a great idea . . . .

Mike O'Keefe
--------------------------------------
} Date: 5/23/95 6:39 AM
} To: Michael OKeefe
} From: hler Martin K
}
} I AGREE with Ed!
}
} Why not separate the microscopy list into one LM list and
} one list containing "the rest" (EM,AFM et.c.)?
}
} People interested in both could of cource subscribe both lists!!
}
} This would solve my, and probably others, problem of too
} many mails that has to be sorted out.
}
} / Martin


} Ed wrote:
} } Dear Nestor's list and Nestor,
} } Since 99% of your posts relate to EM,
} } how about a separate group for Optical
} } Microscopy - I know, the last advances
} } came in 1898 - but a few vendors are coming
} } along with nice "data flow segregated mode"
} } tricks like confocals AND I'd like to buy
} } a used instrument from time to time.
} } (psst. - if any list menmbers are selling,
} } please let me know)
} }
} } Thanks,
} }
} } Ed Monberg
} } LMDC










From: mk-at-enk.ks.se (Martin K|hler)
Date: Tue, 23 May 1995 21:54:10 +0200
Subject: Re: EM vs OM

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Again, about splitting or uniting lists,

I hate to fill up your mailboxes if not necessary, but I need to defend
my standpoint further.

The fact is that most of the conversation in this list is concerning specific
technical EM-questions. Then it is natural to use a common list for material
and life sciences, since the techniques are common.

Of cource lots of you use several techniques besides EM, but then you may be
interested in more than LM. Probably you are interested in computers (in
general), software (several kinds), image analysis (in general), laboratory
equipment (in general), statistics (in general), histology (in general),
chemistry (in general), biology (in general), biochemistry, medicine,
physics...
Do you believe that we should unite all kinds of lists that the subscribers
to this list could possibly be interested in? I guess the answer is NO.

I also believe that one always has to use the "quick delete finger", but I
don't believe that you necessarily need to unite all different part of
microscopy for all discussions. Even if "the international trend is to reunite
again the people", I don't believe that all people will or want to participate
in all kinds of discussions.

Jay Jerome talked about the confocal microscopy list and that "much of the
information there is cross posted here". Maybe some of it is cross posted, but
definetly not that much. Actually a lot on that list concerns quite general
imaging and light microscopy questions that will not be posted to the
microscopy list.

I don't have a good suggestion at this point, but I have something:

1. Maybe there could be one list dealing with questions that may be of interest
to ALL kinds of microscopy. This could be the fertilizing forum between
different kinds of users. Topics like image analysis, immunostaining and other
techniques USED IN SEVERAL TYPES of microscopy can reside here.

2. One other list already exist, namely the confocal microscopy list. I believe
that a part of the discussions on that list could be held on the suggested list
above. Maybe this could be changed into a general LM list (including confocal
microscopy and specific questions for LM).

3. A list for EM-techniques could be used for SPECICIC EM-QUESTIONS (like
tripod polishing and epoxy resins).

This of cource requires one additional list, and I cannot say who should
administer it, I just want to see what you believe. Apparently you are negative
to this now, but is it really so?

/ Martin





From: Fermin, Cesar :      fermin-at-tmc.tulane.edu
Date: 23 May 1995 18:46:17 -0600
Subject: RE: LM vs. EM vs. whatever

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Agree 100%. When a subject heading is part of the message unwanted messages
are deleted up front. In fact, many users of the server probably do not
realize that only a few out there have the time to open messages without a
heater reference. If users want full attention, stick to the subject heater
or message may go directly into the "curved" receptacle, at least that is what
I do.
________________________________________________________

As much as I hate to jump in on these arguments, this one is too close to my
own interests to avoid.
Dr. Gorski has just reminded us of the system Nestor came up with ages ago -
specific subject lines. They seem to work for a while, then fade away again.
Why not just start using them again and STICK WITH IT, this time?
Yes, the volume of mail gets to be a pain, but I've picked up a LOT of
valuable information from this group - I'm sure I'm not the only one who has
a handly little file of tips and techniques, saved from various discussions.
The volume on this list isn't really that heavy, compared to most of the other
groups I've been in.
Why not let Nestor decide what he feels to be appropriate? He is the one who
has to babysit this thing, after all! (And the effort is MUCH appeciated).
Sorry - long day and I had to shoot my ...um...fingers off, I guess.
Tamara Howard
U. of Pittsburgh School of Medicine
Pgh, PA





From: Kara Nakata :      muskrat-at-u.washington.edu
Date: Tue, 23 May 1995 21:09:41 -0700 (PDT)
Subject: unsubscribe

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please unsubscribe




From: f705geri-at-mbox.tu-graz.ac.at (Gerald Kothleitner)
Date: Wed, 24 May 1995 08:25:46 +0200
Subject: TRIPOD Polishing

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I have heart that there is a Tripod Polisher user=B4s group meeting at the
MSA Conference in Kansas City. Unfortunately it is not possible for me to
go there, but if anyone could give me
some literature information on this topic, I would be more than happy.
Thanks in advance.

DI Gerald Kothleitner, FELMI, Steyrergasse 17, A-8010 Graz, Austria, Europe






From: f705geri-at-mbox.tu-graz.ac.at (Gerald Kothleitner)
Date: Wed, 24 May 1995 09:12:23 +0100 (BST)
Subject: Re: EM vs OM

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Message-Id: {199505240812.JAA06193-at-zeus.bris.ac.uk}

} In my humble opinion good subject lines and a quick delete finger are the
} only real solutions to the "too much e-mail" problem.
}
} Jay Jerome

I agree. I thought all this came up soon after the group got started and
we were supposed to preface the subject lines with suitable acronyms -
EM, TEM, SEM, STM, OM, AFM, ........ Then you can quickly delete anything
you think will be of no relevance to you.

And if you think there is a lot of post here that's hard to wade through, you
should try joining feline-l

--
Keith R. Hallam
Research Associate

University of Bristol, |
Interface Analysis Centre, | Telephone: National (0117) 925 5666
Oldbury House, | International + 44 117 925 5666
121, St. Michael's Hill, | Facsimile: National (0117) 925 5646
Bristol, | International + 44 117 925 5646
BS2 8BS, | E-mail: k.r.hallam-at-bristol.ac.uk
England | URL: http://zeus.bris.ac.uk/~phkrh/




From: PRING :      richard.pring-at-bbsrc.ac.uk
Date: Wed, 24 May 1995 04:29:25 -0500
Subject: resin etching of Spurr

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Chris Gilpin,
I tried etching Spurr a long time ago for rust infected plants (J.Micros.
103,289, 1975. I attached the block to an SEM stub with araldite(fiirst score
the stub deeply for good adhesion) and dripped 2.5% Na ethoxide onto the
surface from a syringe. I made up, the etoxide by dissolving metallic sodium in
dry etoh, about 10 ml was all that was needed to make up. This has the
advantage that the stub can be held in an ultratome chuck and faced off with a
glass knife, and semi thins can be cut for LM or u.thins for TEM until an area
of interest is found, and then the surface can be etched. After SEM
examination, the block can be further sectioned. After etching it is importanr
to wash the surface with a jet of ETOH from a wash bottle and dry with a flow
of air. Etcing took about 3 - 5 mins with a drip on the surface every 10s with
the progress being checked with a stereo microscope. A quicker and much more
exciting alternative is to hold the surface to be etched in 5ml of just boiling
etcing solution for about 5s ! This has worked well with various fungal
infected leaves and stems. Good Luck.

Richard Pring
IACR Long Ashton Research Station
Long Ashton
Bristol
BS18 9AF
UK

richard.pring-at-bbsrc.ac.uk




From: Dr. Molnar Peter :      MOLNARP-at-lib.dote.hu
Date: Wed, 24 May 1995 12:22:51 +100
Subject: human anti-renin

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I would highly appreciate if anybody could help me to find a
commercial source that provides antibodies against renin. I am
particularly interested in using EM immunoshistochemistry but LM
applications are also important. Please help if you can!
Thanks
Peter
xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
Peter Molnar, M.D., Ph. D.
Hungarian-Japanese EM Ctr.
Dept. Pathology, Univ. Med. Sch.
Debrecen
Nagyerdei krt. 98., P.O.Box 24.
Fax: 36-52-417-063
e-mail:molnarp-at-lib.dote.hu
xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx




From: dietmar.reiter-at-uibk.ac.at (Dietmar Reiter)
Date: Wed, 24 May 1995 12:43:32 +0100
Subject: Find confocal listserver...

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Hi all,
anyone out there to know the correct SERVER ADDRESS of the CONFOCAL mailing
list (and the server address)? The address {listserv-at-ubvm} seems to be
incorrect. Thanks for help, sincerely,

:-)

+++ Dietmar Reiter, Dept. of Zoology and Limnology
+++ University of Innsbruck
+++ Technikerstrasse 25, A-6020 Innsbruck, Austria
+++ phone: (43)-512-507 ext. -6170, fax ext. -2930






From: smithj-at-acad.winthrop.edu
Date: Wed, 24 May 1995 07:43:16 -0400
Subject: TEM prep: Propane Jet Freezer protocol and manual

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TEM folk. I have inherited a Balzers CryoJet freezer in an attempt
to get well-fixed material of some freshwater flatworms. Would somone
who has used one of these (Balzers QFD 020, or similar propane-jet
freezer) e-mail me personally with some hints?
Julian Smith III
Biology
Winthrop University
Rock Hill, SC
smithj-at-winthrop.edu
803-323-2111 vox
803-323-2246 fax




From: Ronald LHerault :      lherault-at-acs.bu.edu
Date: Wed, 24 May 1995 08:52:15 -0400 (EDT)
Subject: Replicas at 2000x-Proof

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Last week or so, I suggested replication of a sample that proved
difficult to CPD. My belief that you could image details at 2000x was
met with some skepticism so I prepared a test sample of Knoop
indentations on an aluminum stub. I will gladly send copies of the SEM
showing original and replica at 2000x for evaluation of the technique.

Ron





From: Elinor Solit :      cambrex-at-world.std.com
Date: Wed, 24 May 1995 10:08:16 +0059 (EDT)
Subject: Re: TEM prep: Propane Jet Freezer protocol and manual

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Suggest you talk to George Lane at Bal-Tec. 800-875-3713.

Hope this helps.

Ellie Solit
The Microscope Book

On Wed, 24 May 1995 smithj-at-acad.winthrop.edu wrote:

} TEM folk. I have inherited a Balzers CryoJet freezer in an attempt
} to get well-fixed material of some freshwater flatworms. Would somone
} who has used one of these (Balzers QFD 020, or similar propane-jet
} freezer) e-mail me personally with some hints?
} Julian Smith III
} Biology
} Winthrop University
} Rock Hill, SC
} smithj-at-winthrop.edu
} 803-323-2111 vox
} 803-323-2246 fax
}




From: Elinor Solit :      cambrex-at-world.std.com
Date: Wed, 24 May 1995 10:18:39 +0059 (EDT)
Subject: Re: LM vs. EM vs. whatever

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Dear Colleagues,

As the opinions on a light microscopy segment fly back and forth, I have
a request.

Our technology newsletter, Microscope Technology & News/Analytical
Consumer is about to undertake a survey on light microscopes. It will be
similar to the ones we have done on SEM and TEM. If any of you would like
to be included in the survey which is by telephone, and not unduly long,
please send your name and phone number. Additionally, if there are topics
that you would like to see included, tell us that too.

All participants will receive a complimentary copy of the Survey and a
free cup of coffee at MSA in Kansas City. Please let us hear from you
soon; no later than June 7th.

Many thanks.

Ellie Solit
The Cambrex Group
33 Broad St.
Boston, MA 02109
Phone: 617-742-0311
Fax: 617-742-4942
email: cambrex-at-world.std.com





From: Niemczura, Zofia E. :      ZENIEM-at-Inland.com
Date: Wed, 24 May 1995 14:21 -0500 (CDT)
Subject: TEM software

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Hi Microscopist

I am looking for good software for the TEM diffraction pattern
interpretation, providing unknown phase identification on the base of
interplanar angle and spacing measurements, drawing and indexing the
reciprocal lattice, and stereographic projection, and facilitating to define
the crystals orientation. Does anyone know where I can get such a software.
I would greatly appreciate any advice or recommendation.

Zofia Niemczura
3001 East Columbus Dr
Inland Steel Research Laboratories
East Chicago, IN 46312
zeniem-at-inland.com




From: Dwight Beebe :      beebed-at-ERE.UMontreal.CA
Date: Wed, 24 May 1995 15:27:46 -0400 (EDT)
Subject: TEM: contrast in negs

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Hi,
I'd like to know whether any of you have taken the perhaps
overly-AR approach of measuring the OD of your negs with a densitometer?
We've been getting used to the simultaneous installation of a "new" H600
and darkroom equipped with an enlarger (Omega D6) and chemicals that
we've not used before. In trying to get the exposure adjusted for the
scope to give us, on average, a contrast level that will be right for a
Polycontrast 2 filter and reasonable f-stop and exposure times, we've
discovered that negatives with a contrast range of around 0.9 OD, and a
maximum of around 1.9 or 2.0 will yield sufficiently contrasty prints
with a PC2.5 filter. In reading both Agar et al and Meek, we found that
negs of this range should require either Hard paper or probably the PC4.5
filter. Obviously, in practice, it just ain't so. BTW, the exposures
are around 7.5 x 10E-11 A/cm2 -at- 4 seconds, 50 kV. The enlarger has a
150W bulb and we're using the 150mm lens, typically at f16 and about 6
seconds. These conditions are not a rule, just what seemed appropriate
for this particular batch of negatives.
A final bit of information: our material is plant tissue, fixed
in GA and OsO4, some en bloc stained followed by lead citrate staining of
the section, other sections are stained with 3% UA in 30% EtOH or MetOH
and lead citrate (Reynold's) at room temp. Resins are Spurr's
formulation or Epon-Araldite according to Mollenhauer.
After that lead-up, I'd like to hear opinions on what others
consider to be the qualitative and quantitative description of the
"ideal" negative.
Many thanks!

PS: I vote for the unsplitting. I've learned a tremendous amount about
_microscopy_ here and would hate to diminish the diversity of contributors.

Dwight Beebe
Institut de recherche en biologie vegetale beebed-at-ere.umontreal.ca
Dept. de sciences biologiques Voice:514-872-4563
Universite de Montreal FAX:514-872-8496
4101, rue Sherbrooke est
Montreal, PQ H1X 2B2 Canada







From: Michael Boucher :      BOUCHER-at-tcrca.usbm.gov
Date: Wed, 24 May 1995 15:36:43 CST
Subject: Possible Digest form for Microscopy?

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One solution to those who want to remain, but not get a lot of
e-mail they have no interest in would be to offer a digest form with
one large post a day as many other listservers offer. With proper
subjects headers, mail can be quickly skipped over to posts of
interest. That way for those who pay by the message, it would be
less expensivel. Another alternative is the microscopy news list which
a lot of these posts also go to. For those that have no easy news
access, the digest would perhaps be an alternative to losing their
expertise by unsubscribing due to the larger number of posts.
*****************************************************************
Michael L. Boucher Sr. Boucher-at-TCRCA.USBM.GOV
Geology-Mineralogy/Chemistry Labs Ph 612-725-4614
Twin Cities Research Center Fax 612-725-4527
U.S. Bureau of Mines Center 725-4500
Department of Interior
5629 Minnehaha Avenue South
Minneapolis, MN 55417-3099
U.S.A.
*****************************************************************





From: William Tivol :      tivol-at-wadsworth.org
Date: Wed, 24 May 1995 16:42:21 -0500 (EDT)
Subject: TEM: contrast in negs (fwd)

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} Dwight Beebe {beebed-at-ERE.UMontreal.CA} wrote:
} Subject: TEM: contrast in negs
}
} Hi,
} I'd like to know whether any of you have taken the perhaps
} overly-AR approach of measuring the OD of your negs with a densitometer?

Since we do a lot of tomography, a lot of negs get quantitated--
I guess that might be AR, but not overly so. I, myself, do quantitative
electron diffraction, so not only do I quantitate the negs with a PE 1010A
microdensitometer, I have taken a series of exposures to relate the OD to
the number of electrons per pixel. Furthermore, our shop is constructing
a device to calibrate the developing process by exposing one edge of the
film to a set of LED's--John Turner at the NCEM at Berkeley showed me
his device and sent me pictures and schematics, and our device will be a
decendant of his.

} In trying to get the exposure adjusted for the
} scope to give us, on average, a contrast level that will be right

An advantage of densitometry is that the contrast-adjusted files
can be written on film or printed.

} After that lead-up, I'd like to hear opinions on what others
} consider to be the qualitative and quantitative description of the
} "ideal" negative.
}
The "ideal" negative is one which will have the information
*you* need (and the prints, if any, should easily display this in-
formation). This can vary all over the lot. An example is an image
taken in a glass micropipette. We have observed specimens from patch-
clamping--which are across the opening of the pipette--and specimens
contained within the pipette. In order to have proper visualization
of the specimen in the pipette, the area outside of the pipette has
to be at OD ~ 6. Trying to get the tomography programs to deal with
this can be tricky. ED patterns also have a large contrast range--
from } 4 to {0.01 OD, which have to be digitized accurately. Most
"ordinary" images seem to range between ~0.1 and 2 OD, but as EM
becomes more quantitative, such details as matching the OD's for
various tilt angles, etc., will possibly necessitate different limits.
Yours,
Bill Tivol




From: William Tivol :      tivol-at-wadsworth.org
Date: Wed, 24 May 1995 16:52:44 -0500 (EDT)
Subject: TEM software--diff pattern interp

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Zofia Niemczura asked about software for the interpretation of ED patterns.

Dear Zofia,
It sounds like you want something like CRISP. I use a module in the
SPIDER image processing program, but it only gives the information from the
pattern, not the ability to match that info to a short list of possible
unit cells. I can put you in touch with Joachim Frank if you want info
about SPIDER--it was written here and is available for $$$. Good luck.
Yours,
Bill Tivol




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Wed, 24 May 1995 15:09:14 -0500 (CDT)
Subject: Digest MOde & Newsgroups

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G'day Subscribers....

Just a quick note on the recent discussion.

Digest mode is NOT available in the current listserver. I plan
to add it in a new incarnation of the listserver (someday).

Also the Microscopy NEWSGROUP does not echo this mailing list
that feature disappeared several months ago when our gateway
to the newsgroups was taken off line.

In the mean time, let me remind everyone to Use SUBJECT LINES
on your EMail. That is the best short term solution.

Nestor...

Your Friendly Neighborhood SysOp




From: richard.easingwood-at-stonebow.otago.ac.nz (Richard Easingwood)
Date: Thu, 25 May 1995 09:54:35 +1100
Subject: TEM immunocytochem of whole milk

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Mime-Version: 1.0
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Allan Mitchell writes:

I have had an enquiry about performing TEM immunocytochemistry on the fat
in whole milk concentrate which is approximately 15% fat.
The problem is that unless osmium fixation occurs first the fat droplets
collapse. I have suggested to the interested person that cryofixation /
cryosubstitution may be the answer but I really have no experiance with
this type of sample.

Any suggestions or protocols would be greatly appreciated.

Greatly appreciated,

Allan Mitchell


Richard Easingwood
South Campus Electron Microscope Unit
Otago Medical School
PO Box 913
Dunedin
NEW ZEALAND

Telephone: 64-03-479 7301
Facsimile: 64-03-479 7254

"The southernmost electron microscope unit in the world"






From: lmiller-at-ux1.cso.uiuc.edu
Date: Wed, 24 May 1995 16:49:53 -0700
Subject: Re: Possible Digest form for Microscopy?

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Message-Id: {199505242147.AA08011-at-ux1.cso.uiuc.edu}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"


Hello fellow microscopists,

Re Possible Digest --

I subscribe to the Double Reed Society (oboe and bassoon) out of ? Colorado .

This group offers the digest,and it is a real nice feature. I can just zap
one or 2 files off to a disc and take it home to read at my leasure. Only
takes up 1-2 slots in my mail box each day.

In the Double Reed, changing back and forth to and from digest is
(as I understand it) an automated function, not the listsever system op's
hands on work.----but I could very well be wrong.

Active microscopy listservers could choose this option for just when they
leave for meetings and vactaions instead of unsubscribing and missing back
issues and causing (?) the systems operator more work.

If the info's any help:
X-Listprocessor-Version: 6.0a -- ListProcessor by Anastasios Kotsikonas
X-Comment: Message From: listserv-at-acc.wuacc.edu

Lou Ann



=================================

"Michael Boucher"wrote:
One solution to those who want to remain, but not get a lot of
e-mail they have no interest in would be to offer a digest form with
one large post a day as many other listservers offer. With proper
subjects headers, mail can be quickly skipped over to posts of
interest. That way for those who pay by the message, it would be
less expensivel. Another alternative is the microscopy news list which
a lot of these posts also go to. For those that have no easy news
access, the digest would perhaps be an alternative to losing their
expertise by unsubscribing due to the larger number of posts.
==========================================================

***************************
Lou Ann Miller MT(ASCP) CT(MSA)
Microscopic Imaging Lab
College of Vet. Medicine
University of Illinois
2001 S Lincoln Ave
Urbana,Illinois 61801
217-244-1566
lmiller-at-ux1.cso.uiuc.edu






From: Niemczura, Zofia E.
Date: Wednesday, May 24, 1995 2:21PM
Subject: TEM software

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Hi Microscopist

I am looking for good software for the TEM diffraction pattern
interpretation, providing unknown phase identification on the base of
interplanar angle and spacing measurements, drawing and indexing the
reciprocal lattice, and stereographic projection, and facilitating to define

the crystals orientation. Does anyone know where I can get such a software.

I would greatly appreciate any advice or recommendation.

Zofia Niemczura
3001 East Columbus Dr
Inland Steel Research Laboratories
East Chicago, IN 46312
zeniem-at-inland.com




From: John Millar :      jjmill-at-RMIT.EDU.AU
Date: Thu, 25 May 1995 09:34:28 EST-10
Subject: Re: EM vs OM, splitting the list,etc

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Jay Jerome {jjerome-at-isnet.is.wfu.edu}

To list members :

Jay Jerome wrote :
} A too full mail box will always be a problem.......
} In my humble opinion good subject lines and a quick delete finger are the
} only real solutions to the "too much e-mail" problem.
..........

I agree The list does provide a good cross-section of information and
delete works well. The previous suggestion of coding for content
could work but if authors are intelligent about the subject
indicated it should not be necesssary and one can delete messages of
lower relevance without losing important information.
cheers
jjm
Professor John J. Millar
Head, Department of Applied Physics
Electron Microscope Unit
RMIT, GPO Box 2476V, MELBOURNE AUSTRALIA 3001
+613 660 2602 fax 613 660 3837
email jjmill-at-rmit.edu.au




From: bozzola-at-siu.edu (John. J. Bozzola)
Date: Wed, 24 May 1995 19:12:37 -0600
Subject: PC Control of Hitachi 7100

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Hello EM Folks -

Anyone have experience using a PC to control the Hitachi H7100 TEM? I know
that a program is available to control most of the TEM functions using an
IBM-type computer. What are your impressions? Also, I am interested in
using a PowerMac 8100 in this capacity and was wondering if anyone has used
one here.

Thank you kindly -


John J. Bozzola
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
Phone: 618-453-3730
Fax: -2665
Email: bozzola-at-siu.edu OR bozzola-at-qm.c-cem.siu.edu






From: vicenzi-at-phoenix.Princeton.EDU (Ed Vicenzi)
Date: Thu, 25 May 1995 09:03:36 -0500
Subject: Imaging zirconia grainboundaries

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My experience is that the cathodoluminesence technique will produce the
best result for imaging ZrO2 grain boundaries that are "invisible" to
secondary electrons.



} the following is the original message
} From: PO2::"ubira-at-iqm.unicamp.br" "Ubirajara Pereira Rodrigues Filho"
} 23-MAY-1995 08:35:46.24
} To: microscopy-at-aaem.amc.anl.gov
} CC:
} Subj: TEM-cellulose acetate porous membranes preparations
}
} Dear Net's;
} Can someone help me? I'm working with cellulose acetate porous
} membranes coated with a thin film of zirconium dioxide. I was studying
} this membranes with SEM X-ray microanalysis system to study the
} distribution of the oxide on the membrane surfaces. This task was
} succesfull, but when we try see the grain boundary we can't. Then we
} solve use the TEM for this pourpose. We embebed the membrane in a epoxi
} resin to cut in a ultramicrotome, but the membrane structure is collapsed
} and we lost the porous structure.
} Thank you in advance,
}
} Ubirajara Pereira Rodrigues Filho
} Instituto de Quimica- UNICAMP
} Campinas, SP, Brazil, 13083-970, CP 6154
} e-mail: ubira-at-iqm.unicamp.br

Ed Vicenzi tel (609) 258-1464 office
Princeton University tel (609) 258-1406 lab
Princeton Materials Inst. fax (609) 258-6878
70 Prospect Ave.
Princeton, N.J.
08540-5211 vicenzi-at-princeton.edu






From: Sally Shrom :      sally-at-retina.anatomy.upenn.edu
Date: Thu, 25 May 1995 08:58:21 -0400 (EDT)
Subject: DAB and Epon

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Posted-Date: Thu, 25 May 1995 08:58:54 -0400

Has anyone had epon embedding problems following the use of DAB?
Sally Shrom
sally-at-retina.anatomy.upenn.edu




From: Katherine.S.Connolly-at-Dartmouth.EDU (Katherine S. Connolly)
Date: 25 May 95 10:12:57 EDT
Subject: Bio/TEM,DAB and EPON

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keep the list united--please
I have united DAB and LR White with no problems.
Kate Connolly




From: bob-at-shiloh.nimh.nih.gov (Bob Cohen)
Date: Thu, 25 May 1995 10:13:15 -0400
Subject: Help with Locating old Nikon Equipment

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X-Sender: bob-at-shiloh.nimh.nih.gov
Mime-Version: 1.0
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Dear Net's:
We would like to start doing some quantitative fluorescent
microscopy, but have limited funds for establishing an appropriate system.
Nikon representatives have informed us that they might be able to help us
create an analogue system using our current Nikon microscope if we could
locate enough discontinued equipment. They suggested that we try to contact
scientists who might for a variety of reasons be no longer in need of these
parts:
#96969: Dual photometer attachment
#86950: P1 Photometer w/FASTINCA INDO-1 Calcium Software
#86953: FX Photometer control unit
Thanks, in advance, for your help


Robert Cohen: Tel.: 301-402-4925
Fax: 301-480-1668
e-mail Bob-at-shiloh.nimh.nih.gov





From: bob-at-shiloh.nimh.nih.gov (Bob Cohen)
Date: Thu, 25 May 1995 15:44:55 +0100 (BST)
Subject: No microscopy: Cats

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Message-Id: {199505251444.PAA14824-at-zeus.bris.ac.uk}

Apologies for sending this to the list, but I accidently deleted the
message I was going to reply directly to. Someone asked what feline-l was.
Its an e-mail discussion group about cats.

To subscribe to FELINE-L one must:

Send E-mail to:

{LISTSERV-at-PSUVM.BITNET} for Bitnet users,
{LISTSERV-at-PSUVM.PSU.EDU} for Internet users,

with a blank subject line and the following command as the
first (and only) line of the message body:

SUBSCRIBE FELINE-L Your_Full_Name

replacing "Your_Full_Name" with your real, full, true name.

To send, or "post", a message on FELINE-L so that it will be
seen by all subscribers, proceed thusly:

Once subscribed, send FELINE-L postings as E-mail to:

{FELINE-L-at-PSUVM.BITNET} for Bitnet users,
{FELINE-L-at-PSUVM.PSU.EDU} for Internet users,

--
Keith R. Hallam
Research Associate

University of Bristol, |
Interface Analysis Centre, | Telephone: National (0117) 925 5666
Oldbury House, | International + 44 117 925 5666
121, St. Michael's Hill, | Facsimile: National (0117) 925 5646
Bristol, | International + 44 117 925 5646
BS2 8BS, | E-mail: k.r.hallam-at-bristol.ac.uk
England | URL: http://zeus.bris.ac.uk/~phkrh/




From: kennedy-at-nsi.edu (grace kennedy)
Date: Thu, 25 May 1995 08:43:37 -0800
Subject: BIO/TEM/DAB

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I have embedded DAB-reacted material in EPON, ARALDITE, QUETOL 651, and LR
WHITE with no resin problems associated with the DAB. G.Kennedy, TLHe
Neurosciences Institute, La Jolla, CA






From: Patty Jansma :      plj-at-manduca.neurobio.arizona.edu
Date: Thu, 25 May 1995 08:55:32 -0700 (MST)
Subject: Re: DAB and Epon

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We have not had any problems with epon embedding after DAB.

Patty Jansma Tel:602-621-6671
plj-at-manduca.neurobio.arizona.edu
Arizona Research Labs Division of Neurobiology
University of Arizona


On Thu, 25 May 1995, Sally Shrom wrote:

} Has anyone had epon embedding problems following the use of DAB?
} Sally Shrom
} sally-at-retina.anatomy.upenn.edu







From: jjordan-at-world.std.com (Jo Rita Jordan)
Date: Thu, 25 May 1995 12:18:06 -0400
Subject: Optical microscopy survey

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Microscopists:

Analytical Consumer is doing a survey of users of optical microscopes and
solicit contributions from this mail list. It will appear in our July
issue, and all participants will receive a copy of the survey results.

Analytical Consumer, which merged in February with the newsletter,
Microscope Technology & News (MT&N), surveys users of a different
laboratory technology each month. We report on customer satisfaction with
the equipment, keeping the names of our sources confidential. You will not
be asked to buy anything; our surveys are for the use of our subscribers,
and you will receive the July issue. Our subscribers are large laboratories
in industry, government, and environmental testing worldwide.

We published surveys of SEM and TEM in the past, in cooperation with MT&N.
Several members of this mail list participated in the TEM survey.

I hope that many of you will be interested in participating. Send me e-mail
and let me know if you are. I'll send you the short list of questions for
the survey.

Thanks,

Jo Rita Jordan, PhD
Editor and Publisher
Analytical Consumer






From: Doug Davis :      doug_davis-at-maillink.berkeley.edu
Date: 25 May 1995 09:12:12 -0700
Subject: LM vs. EM vs. whatever

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Subject: Time: 9:03 AM
OFFICE MEMO RE: LM vs. EM whatever Date: 5/25/95

Tamara et al- Hear hear. Exactly right. I follow the same MO and tire of
all the discussion.

On 5/23, Tamara Howard wrote:

} As much as I hate to jump in on these arguments, this one is too close to } my
own interests to avoid. Dr. Gorski has just reminded us of the system } Nestor
came up with ages ago - specific subject lines. They seem to } work for a
while, then fade away again. Why not just start using them } again and STICK
WITH IT, this time? Yes, the volume of mail gets to be a } pain, but I've
picked up a LOT of valuable information from this group - } I'm sure I'm not
the only one who has a handly little file of tips and } techniques, saved from
various discussions. The volume on this list isn't } really that heavy,
compared to most of the other groups I've been in.
} Why not let Nestor decide what he feels to be appropriate? He is the one } who
has to babysit this thing, after all! (And the effort is MUCH } appeciated).
Sorry - long day and I had to shoot my ...um...fingers off, I } guess.
} Tamara Howard
} U. of Pittsburgh School of Medicine
} Pgh, PA






From: baskin-at-biosci.mbp.missouri.edu (Tobias Baskin)
Date: Thu, 25 May 1995 10:08:23 -0500
Subject: allM/photography/prints

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Dear Microscopists,
For making prints of micrographs I have always used
glossy paper. A few weeks ago, we got a box of "pearl" (ie, matt) paper by
accident. I made a bunch of prints with this pearl paper, and they appeared
"different" from prints on glossy paper, besides the lack of shine.

Is there some conventional wisdom about when to use glossy and when
to use matt?
How about when preparing plates for publication: is there a
difference between paper finishes in the way plates get reproduced by
journals?

I would really appreciate comments here, having never had any
formal training in photography, and not being able to find any comments in
the book on photomicrography in my possesion.

Thanks in advance,

Tobias Baskin

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
___ ____ ^ ____ _____ Tobias I. Baskin
/ \ / / \ / \ / University of Missouri
/ | / / \ / / Biological Sciences
/___ / /__ /_____\ / /__ 109 Tucker Hall
/ / / \ ( / Columbia, MO 65211 USA
/ / / \ \ / voice: 314-882-0173
/ /____ / \ \____/ /_____ fax: 314-882-0123






From: Dr. Molnar Peter :      MOLNARP-at-lib.dote.hu
Date: Thu, 25 May 1995 17:35:01 +100
Subject: diamond knife

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Hi Everybody:
I have a diamond knife that is a few months old and has been used
with TLC. There is no damage visible on it even under the highest
magnification that is possible to use on the microtome. I have tried
to clean it with soft wood, balsa (dry and soaked in acetone and 100
% ethanol) and it still gives me headaches. It has been possible to
cut beautiful sections with it, nothing hard has been cut. The only
thing that was a bit unusual for our lab was a series of blocks of
fungi (thick-walled, polysaccharides are abundant in the "hard" wall)
that we cut lately. Now nothing works. We have played with the angle
to no avail. The whole length (2.4 mm) behaves the same way. It is a
Nisshin EM Co., Ltd., Tokyo made knife, we use a Reichert ultrotome.
Has anybody any suggestions? I hate to think about sending it for
resharpening! Any other way to clean a possible submicroscopic sticky
polysaccharide contamination? Please give me a hint! The problem with
the sections is that they come off in pieces as if there was
something along the cutting edge.
Thanks
Peter
molnarp-at-lib.dote.hu




From: MicroToday-at-aol.com
Date: Thu, 25 May 1995 17:12:28 -0400
Subject: Micrograph Prints

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Group -
In regard to this subject, whether there is a preference for glossy or matte
paper for prints, to be used in publications, there would be a very, very
slight preference for glossy. The reason is that with matte, there is a
chance that the color separation process might pick up a bit of the fiberous
surface of matte. As far as color, or resolution, there is no real
difference between the two.
However, and whether the paper print results from laser printing or
photographic reproduction, they are the worse possible option for
publication.
The best option is 4 x 5 inch transparencies - followed closely by 35 mm
slides (particularly when the final image tends to be small - like in normal
journals, etc.). The reason is that these are "first generation" images.
The next option would be the image on disk - not too bad, but they are
"second generation" images - 1: the photography and 2: the scanning.
I hope that some may find the above of interest.
Don Grimes
Microscopy Today





From: JOHNA-at-SCI.WFEB.EDU
Date: Thu, 25 May 1995 16:36:58 -0400 (EDT)
Subject: Re: allM/photography/prints

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Hey Tobias,

I use glossy for just about everything, ie., routines, for publication, etc
. BUT I use a matt finish for posters. The non-glossy surface greatly
reduces glare from hideous, exhibit hall lighting and saves the necks of
those trying to read your poster who would otherwise be engaging in all
sorts of contortions to look a micrographs through the glare. Matt finish
prints do tend to have a softer, warmer presence about them as well which
may sometimes be unsettling.

Have fun

John



___________________________________________________
| |
| John G. Aghajanian, Ph.D. |
| Worcester Foundation for Experimental Biology |
| 222 Maple Avenue |
| Shrewsbury, MA 01545-2737 |
| |
| Tel: 508 842-8921 ext. 147, 161 |
| Fax: 598 842-9632 |
| JOHNA-at-sci.wfeb.edu |
| |
|_________________________________________________|





From: bergrh-at-cc.memphis.edu (R. Howard Berg)
Date: Thu, 25 May 1995 15:55:27 +0600
Subject: Re: allM/photography/prints

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Most journals require glossy prints, since they provide the maximum
"brilliance" or dynamic range of the image. Glossy paper maximizes
reflection of light back off the paper base, giving better contrast.


} Dear Microscopists,
} For making prints of micrographs I have always used
} glossy paper. A few weeks ago, we got a box of "pearl" (ie, matt) paper by
} accident. I made a bunch of prints with this pearl paper, and they appeared
} "different" from prints on glossy paper, besides the lack of shine.
}
} Is there some conventional wisdom about when to use glossy and when
} to use matt?
} How about when preparing plates for publication: is there a
} difference between paper finishes in the way plates get reproduced by
} journals?
}
} I would really appreciate comments here, having never had any
} formal training in photography, and not being able to find any comments in
} the book on photomicrography in my possesion.
}
} Thanks in advance,
}
} Tobias Baskin
}
} - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
} ___ ____ ^ ____ _____ Tobias I. Baskin
} / \ / / \ / \ / University of Missouri
} / | / / \ / / Biological Sciences
} /___ / /__ /_____\ / /__ 109 Tucker Hall
} / / / \ ( / Columbia, MO 65211 USA
} / / / \ \ / voice: 314-882-0173
} / /____ / \ \____/ /_____ fax: 314-882-0123


R. Howard Berg, Ph.D.
Biology Department
University of Memphis
Memphis, TN, 38152
E mail: bergrh-at-cc.memphis.edu
phone: 901-678-4449 fax: 901-678-4457







From: Jay Jerome :      jjerome-at-isnet.is.wfu.edu
Date: Thu, 25 May 1995 17:39:56 -0400 (EDT)
Subject: Re: allM/photography/pri

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The rule of thumb in my laboratory is:

Publication prints on glossy paper: better range.

Poster presentation prints on matte paper: less reflected glare.

This comes from my experience in a previous life as photographers assistant.

Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-isnet.is.wfu.edu *
**************************************************************

On Thu, 25 May 1995, Tobias Baskin wrote:

} Dear Microscopists,
} For making prints of micrographs I have always used
} glossy paper. A few weeks ago, we got a box of "pearl" (ie, matt) paper by
} accident. I made a bunch of prints with this pearl paper, and they appeared
} "different" from prints on glossy paper, besides the lack of shine.
}
} Is there some conventional wisdom about when to use glossy and when
} to use matt?
} How about when preparing plates for publication: is there a
} difference between paper finishes in the way plates get reproduced by
} journals?
}
} I would really appreciate comments here, having never had any
} formal training in photography, and not being able to find any comments in
} the book on photomicrography in my possesion.
}
} Thanks in advance,
}
} Tobias Baskin
}
} - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
} ___ ____ ^ ____ _____ Tobias I. Baskin
} / \ / / \ / \ / University of Missouri
} / | / / \ / / Biological Sciences
} /___ / /__ /_____\ / /__ 109 Tucker Hall
} / / / \ ( / Columbia, MO 65211 USA
} / / / \ \ / voice: 314-882-0173
} / /____ / \ \____/ /_____ fax: 314-882-0123
}
}
}




From: modum-at-gatan.com (Michael Odum)
Date: Thu, 25 May 1995 13:02:40 -0700
Subject: Re: TEM sample preparation.

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Message-Id: {199505251901.MAA19531-at-core.gatan.com}

Hello,

This is going to sound a lot like a commercial, so I apologize now if I
offend anybody. My only intention is to inform you of new developments you
may not be aware of and of my experiences with the Gatan's ion mills.

To answer Mr. Peiro's questions about the PIPS the main advantage it has
over the DuoMill is focused ion gun milling at low angles. It is not to be
confused with a Focused Ion Beam (FIB) system. The PIPS can not do that
kind of precission milling as it is designed for a wider range of materials
and functions. It is fitted with new variable angle guns which means you
can mill a specimen from the top and/or bottom at +10o to a theoretical 0o.
New specimen mounting posts called the DuoPost have just been released to
take full advantage of these guns.The posts are fully compatible with the
DuoMill.

The DuoMill is capable of milling at 0o (with the DuoPost) but it is
what we call a broad-beam system. This means the ions are spread out over a
larger area reducing effectivness. The DuoMill is a very good instrument
for situations where there are a lot of users at the same time who have
different needs. A classroom of future materials engineers is a good
example. The biggest disadvantage is time, because in comparison to the
PIPS, the PIPS is almost twice as fast. Also the ion beam will erode the
apperatures where the PIPS guns do not have that problem.

As to maintenance on the PIPS there are only a few simple things that
will be required on a regular basis. The guns will need to be checked for
alignment daily, especially if there will be multiple users, but I find
that if you check in the morning you can forget about it for the rest of
the day. The O-ring in the airlock assembly should be cleaned and regreased
about once a week to provide smooth operation of the airlock piston. I
recommend cleaning off the sputtered material on the viewing window at the
same time, and you can do this with flux remover or, I use, a small amount
of medium grade diamond paste and rub the sputter off. About once a month
you will want to clean the window in the specimen mount assembly in the
airlock which is done th same as for the viewing window.

The uncommon things that you will do eventually will be to clean the
gun/s when it/they short out. You would simply vent the unit, remove the
gun/s, take the magnets apart, and use a little compressed air to blow any
loose material out. Do not use any sort of solvent for this, especially
acetone. It will take three times as long to bring the unit back to an
aceptable vacuum and acetone will dissolve the plastic components of the
guns. The other thing is the built-in diaphram-pump requires a small oil
cartridge for the bearing material and that has to be replaced every 5000
hours. (The oil never enters the vacuum system.) All these things are
described in detail in the operating manual.

May I make a suggestion as to dealing with InP? If you do decide on a
PIPS then it would be a good idea to get a CAIBE (Chemically Assisted Ion
Beam Etching) accessory. When I work with InP I find the ion beam produces
In rich "islands" artifacts which is a pain, but a CAIBE loaded with iodine
crystals will sublime enough gas to dissolve these "islands" as they form
under ther ion beam. The iodine also tends to increase the milling rate for
InP at an average of 1um per minute.

Sorry about the length. If you have any questions please contact me. Thanx.

Michael Odum
Spec. Prep. Tech.

Gatan, Inc.
6678 Owens Dr.
Pleasanton, CA 94588
Tel: 510-463-0200
Fax: 510-463-0204
E-Mail: modum-at-gatan.com

} Hi,people.
} We are interested in buying a new equipment for sample preparation (InP
} is our great "nightmare"). It seems that the ion bombardment system PIPS
} (from GATAN) works well and fast. However, before taking a decision, we
} would like to know the opinion of the users. We would be very grateful if
} anybody could inform us about the following points: how well does it work?,
} which are the main advantages with respect to the GATAN DUO MILL system?
} and, what about the maintenance requirements of the equipment?.
}
} Thank you.
}
} Francesca Peiro
} _________________________________________________________________
}
} Unitat ESCA-TEM
} Serveis Cientifico-Tecnics Carrer Sole i Sabaris, s/n
} Universitat de Barcelona 08028 Barcelona, Espana
}
} Tel +34 3 402 16 95
} Fax +34 3 402 13 98
} _________________________________________________________________





From: Diana_Papoulias-at-nbs.gov
Date: Thu, 25 May 1995 18:03:52 -0600
Subject: LM/EM-help locating parts

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Mime-Version: 1.0

I am a fishery biologist and will be going to Russia in {1 month to
work with a microscopist. I have just been asked to bring parts for
their ultratome and electron microscope and haven't a clue where to
start looking. If you can direct me to sources for the following I
would greatly appreciate it. Thank you in advance. Reply to
Diana_Papoulias-at-nbs.gov or FAX: 314-876-1896 or PHONE: 314-875-5399.

She has asked me for:

Luminescent lamp for Ultratome III, LKB, Sweden, type TL 4W/33
Phillips 6

Thin foil apperture for C.L. JEM - 100 c (JEOL, Japan)
Thin foil apperture for O.L. JEM - 100 c




From: linton-at-pisces.rutgers.edu (Linton)
Date: Thu, 25 May 1995 20:37:50 -0400 (EDT)
Subject: SEM of Neurons

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A friend is trying to study the primary auditory neurons using SEM,
the neurons are grown in fetal calf serum with BDNF on thermonox cover
slips. These are then fixed in 2% glut 0.5M phosphate buffer pH 7.0 for
upto 1.5 hours and post-fixed in 2% OsO4 same buffer, dehydrated in EtOH
followed by CPD and sputter coating.
The problem is that according to them what they see under the SEM
does not look like what they see in the LM before fixation. The Neurons are
apparently gone and only swann cells and fibroblast are left.
Does anyone have a fixation protocol that will preserve the neurons?

Any and all help appreciated.

TIA

____________________________________________________________________________
Eric Linton
Rutgers, The State University of New Jersey
Cell and Developmental Biology
e-mail: linton-at-pisces.rutgers.edu
snail mail:
Rutgers/Nelson Labs C-109
P O Box 1059
Piscataway, NJ 08855-1059






From: Henrik Guldberg Pedersen :      imi.dtu.dk-at-unidhp.uni-c.dk
Date: Fri, 26 May 1995 10:42:54 +0000
Subject: RE: AFM, particles

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To all AFM microscopists!

I have had problems examining samples consisting small particles
using the AFM. It seems like the particles are pushed around by the
tip.
Does anyone have any good ideas to solve this problem i.e. to hold
the particles fixed - my small ceramic particles are usually 5 - 250
nm in diameter.
Any comment is appreciated.

Kind Regards
Henrik Guldberg Pedersen
Institute of Mineral Industry
Technical University of Denmark, bld. 204
DK-2800 Lyngby
Phone: + 45 45 25 22 47
Fax: + 45 45 93 48 86
E-Mail: imihgp-at-unidhp.uni-c.dk




From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Fri, 26 May 1995 22:04:44 GMT+1200
Subject: microscopy subgroups

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As a brandnew subscriber who received 30 emails within the first 24
hours, I wish to endorse Eugene Smelik's suggestion to include a
three-letter code, in fact my first posting was going to be to ask
all if they knew of any group specialising in my primary interest,
which is electron microprobe for geological materials. I manage and
maintain a 25-year old JEOL JXA-5A probe, with Oxford Instruments
(LINK SYSTEMS as was) EDS system in a geology department in New
Zealand, would love to hear from others in similar endeavours, view
swapping views, tips, problems, STANDARD MATERIALS, etc.
So maybe add EMP to the acronym list?




From: GVKM07A-at-prodigy.com (DR CHARLES A GARBER)
Date: Fri, 26 May 1995 06:50:28 EDT
Subject: Spare parts for LKB unit & 100C JEOL

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-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --

On May 25, Diana Papoulias asked about the following:

1] LKB MICROTOME PARTS: LKB was purchased by Leica, Inc. some years
ago and you can reach them at (800) 248-0123 in Deerfield, IL. If they
don't handle what used to be LKB products out of that location, then
they can tell you where to call.

2] THIN FOIL APERTURES FOR JEOL 100CX: You can call JEOL (USA) on
(508) 535-5900 and ask for their parts department. As of about a year
ago, however, JEOL did not offer these kinds of apertures (to my
knowledge). You should find out from your contact in Russia a) whether
it is gold foil thin film apertures they are seeking (that is probably
what they want), and if yes, then what hole sizes do they want. We at
SPI can supply just about any hole size in a gold foil thin film
aperture down to a hole size of only 1 um [no, that is not a typo!].
But you will have to find out what kind of work they are doing and what
kind of hole sizes they are seeking. One more thing: Some of the 100C
TEMs in Russia were made on an assembly line purchased from JEOL but
were actually made in Russia. We have found that the OD's of the
apertures used in some of these instruments might be different than the
ODs used for the microscopes made in Japan. Therefore it would be good
to confirm the OD's of the apertures that will fit into their aperture
holders, just in case the dimensions are different.

Hope this helps.

PS: Simple items like tweezers and grids are often times hard to find
in Russian EM labs. You might want to take along a few such items
just in case! But don't try to take any chemicals on the plane with
you, it is illegal and also dangerous. Contact me directly if you want
special information on some of the options for getting hazardous goods
to Russia for the lowest possible shipping charges.

Charles A. Garber
PRESIDENT
SPI SUPPLIES
PO BOX 656
WEST CHESTER, PA 19380 USA

Ph: (800) 2424-SPI
(610) 436-5400

FAX: (610) 436-5755
e-mail: GVKM07A-at-prodigy.com







From: NICK SCHRYVERS :      nick_schryvers-at-ruca.ua.ac.be
Date: 26 May 1995 13:40:24 +0100
Subject: micr. subgr.

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Message-Id: {n1410626511.69994-at-ematserv.ruca.ua.ac.be}

REGARDING micr. subgr.

Hi to you all,
In the recent discussion on a separate list for OM I'd like to remind us that
there's currently a trend to expand the national and international societies
for EM towards other techniques. In many cases the "E" of electron in the
acronyms has been dropped. In this view I don't think one should plan
separate groups for separate microscopy techniques. The idea of three letters
in front of the heading would indeed be more helpful.
Nick Schryvers





From: Lars.Bjork :      lasse-at-UKWANGELA.imm2.su.se
Date: Fri, 26 May 1995 17:08:43 +0200 (MET DST)
Subject: Fixation procedure for viruses

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Hi,

I'm interseted in doing IHC on virus infected tissue sample.

What would be the optimal fixation procedure in terms of inactivating the
pathogen and still preserve the morphology and antigenicity of proteins
as well as possible.

Suggestions?


Lars Bjork
Dept. Immunology
Stockholm University
SWEDEN




From: Joseph P. Neilly 708-938-5024 :      NEILLY.JOSEPH-at-igate.abbott.com
Date: Fri, 26 May 1995 10:02:00 -0600 (CST)
Subject: Subscribing Problems/Possible Solution

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Mr-Received: by mta RANDB; Relayed; Fri, 26 May 1995 10:37:27 -0600
Mr-Received: by mta MCM$RAND; Relayed; Fri, 26 May 1995 10:37:28 -0600
Mr-Received: by mta RANDD; Relayed; Fri, 26 May 1995 10:37:42 -0600
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Content-Return: prohibited

Folks,

A possible idea that might reduce the number of people who improperly
try to subscribe or unsubscribe. Maybe our Friendly Neighborhood Sysop
could automatically send a brief message containing the rules for
subscribing and unsubscribing to the list on a regular basis say, once a
week. It should reduce the number of messages improperly sent to
Microscopy requesting to join or quit. What do you think Nestor? Is
this doable without much hassle? Just my two cents worth.

Joe Neilly
Abbott Laboratories
Cellular and Microscopic Research
Abbott Park, IL
E-Mail: Neilly.joseph-at-igate.abbott.com






From: naresh-at-pop.uky.edu (Naresh Shah)
Date: Fri, 26 May 1995 16:47:41 -0400
Subject: Listserv management - FAQ?

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Message-Id: {199505262047.QAA24511-at-service1.uky.edu}
X-Sender: naresh-at-pop.uky.edu
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Mime-Version: 1.0
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I believe Nestor is doing excellent job as a SYSOP and we need to
appreciate his dedication. Recently, there has been lot of discussion
about management issues of the listserv viz., propoer codes in the subject
lines, subscribing/unsubscribing, commercial advertisements etc. (and I am
sure that I shall receive some flame for this message too!).
I am proposing to set up a message which would be posted at
regular intervals (about once a month) summarizing these issues.
Nestor used to send a message like that when people subscribe asking them to
keep it handy. It is obvious that a lot of people are ignoring it and
discarding it.
I hope a regular monthly reminder would encourage people to follow it.
On the same line of thought, may be we can set up a few FAQs. As a new
member on several groups, I have learnt a lot from those FAQs. For a diverse
group like microscopy, may be several FAQs will be appropriate: (1) EM, (2)
OM, (3) Bio sample prep, (4) Material Sc. problems and even (5) a directory
of commercial suppliers of services and products. This could be lot of
work and
would also require regular maintenance. Nestor is too busy and he should not
be imposed upon. I am hoping that a few volunteers can do the preliminary
work and I would volunteer my services for maintaining the FAQs. I can also
try to post the FAQs as well as a file concerning listserv issues on a
monthly basis.
What does the community think of this idea? I do not intend to
increase non
technical discussion on the issue. My motive in positng this message is to
reduce
such discussion in future so that I do not have to waste my time going
through similar messages.

Naresh Shah
CFFLS, 341 Bowman Hall
University of Kentucky
Lexington, KY 40506-0059
(606)257-5119
(606)257-7215 FAX
Alternate e-mail: naresh-at-funky.cffls.uky.edu







From: John Kloetzel :      kloetzel-at-umbc.edu (John Kloetzel)
Date: Fri, 26 May 1995 17:15:44 -0400
Subject: buried in mail??

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As part of this UNSUBSCRIBE message, I would like to get in a
parting comment per the discussion of "OM vs. EM" splitting. As a cell
biologist, I use all forms of microscopy, and increasingly LM, as confocal
becomes an ever more central, almost essential technology. (I still teach
a 'Biological Electron Microscopy' course, however.) It's been noted how
even the local affiliate societies of MSA have also dropped the "Electron"
out of their names; it's basically a non-issue.
What remains, however, is a big split between the
physical/materials microscopists and the biologists. I don't have any idea
about what a "tripod" is, or why one would want to polish one; perhaps if I
were a little less AR I could bleep out suspect titles w/o reading.
However (my problem) I even open up "unsubscribe" messages, just in order
to see if someone (like me) is qualifying their departure with some sort of
rationale. When people vote with their feet, it's nice to get a view into
their minds, not just their backsides!
Basically the volume of mail has become so much that if a skip a
day or two, I feel buried, and am losing key missives from departmental
colleagues about meetings, etc, that get lost in the shuffle. Perhaps if
there were a *biological microscopy* listserv, the volume might be more
managable?
Anyway, hats off to the manager of this list; I can't imagine the
time it must take! Not being in general a 'splitter', but a generalist, I
can't pretend to foist my splitting instinct on this particular issue onto
others. You can't please everyone, nor suit everyone, no matter what you
do, or how well. I'll have to find another (Internet?) way to keep up on
tech chatter, on my own schedule -- or drop into this list occasionally,
intermittently, to browse.
Problem with unsubscribing is I won't see whether I have other
like-minded users out there. (I do accept private e-mail, however.)
Sayonara -- it's been an education.

** JAK **

****************************************************************************
John A. Kloetzel, Ph.D. {kloetzel-at-umbc.edu}
Department of Biological Sciences
University of Maryland Baltimore County (UMBC)
Catonsville, MD 21228-5329 USA
Phone: (410) 455-2247 or -3913 (Lab)
FAX: (410) 455-3875
****************************************************************************








From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Sat, 27 May 1995 22:33:14 GMT+1200
Subject: Re: acronym: EPMA

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} Date sent: Fri, 26 May 1995 09:17:53 -0500 (CDT)
} From: Alfred Kracher {S1.AXK-at-ISUMVS.IASTATE.EDU}
} Subject: acronym: EPMA
} To: microscopy-at-aaem.amc.anl.gov

} I agree with Ritchie Sims about needing a separate acronym for electron
} microprobes, but suggest the (more or less) "official" EPMA, even if it
} is 4 rather than 3 letters. I suggest confining this to contributions
} regarding equipment, analysis, chemistry, etc. related to *wavelength-
} dispersive* techniques, to distinguish it from EDS, energy-dispersive
} analysis, which may have a different circle of readers.
} Alfred Kracher
} akracher-at-iastate.edu
}

Thanks for the agreement, but can I make a plea for EDS-equipped
instruments also to qualify as EPMA? The original WDS system on our
JXA-5A were removed and dumped by a rather cavalier predecessor some
years ago, we have only an (excellent) EDS from Oxford Instruments
(aka LINK SYSTEMS). Goes real good, though, and apart from a
sesitivity disadvantage cf. WDS gives pretty good results for
geological specimens. I run at about 300pA absorbed current, which is
great for volcanic glasses, lose v little Na.

Does anyone have a good standard for potassium (preferably a
silicate) of which they could spare me a grain or two?
I have only a slightly dubious adularia.
And what do other and wiser people use for Si std for silicate
analysis? I feel a bit iffy about quartz.

Ritchie Sims
Geology Department
University of Auckland
Auckland, New Zealand










From: ldm2-at-apollo.numis.nwu.edu (L.D.Marks)
Date: Sat, 27 May 1995 16:04:37 -0500
Subject: EM: Open House

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There will be an open house at Northwestern University on June 2nd
from 1-4 for the new SPEAR facility. This instrument combines conventional
surface science instrumentation such as XPS, Auger, SEM, thin film growth
with a UHV-HREM and is open for outside users. For more information
please contact either:
ldm-at-apollo.numis.nwu.edu
eric-at-apollo.numis.nwu.edu




From: Paul Keltjens :      PAK-at-eo.ie.philips.nl
Date: Mon, 29 May 1995 10:12:47 GMT+0100
Subject: Philips EM Web pages

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PHILIPS ELECTRON MICROSCOPY WORLD WIDE WEB PAGES

We are starting up on the World Wide Web as from May 24th using
this medium to get ever closer to our electron microscope customers.
For that purpose we have designed a number of web pages giving information on
our company, our products and how to contact us in the various parts
of the world. We surely welcome your reactions and suggestions.

To check out the Philips Electron Optics home page open URL:

http://www.wise.nl/peo

Thanks
Paul Keltjens



FOR INFORMATION ABOUT THIS MESSAGE CONTACT:

Paul A.V. Keltjens
Philips Electron Optics
Marketing Communications Department
Building AAE, P.O. Box 218
5600 MD Eindhoven
The Netherlands

Tel. +31 40 76 62 25
Fax. +31 40 76 61 02
Email (internet): pak-at-eo.ie.philips.nl




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Mon, 29 May 1995 8:21:38 -0500 (CDT)
Subject: Administrativia

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G'day Subscribers...

I appreciate the offers for help. If anyone wants to prepare
additional FAQ files that's great. Just go ahead and have
fun, however, I have one request/comment. Remember the old expression:
"Too Many Cooks Spoil the Broth", so just prepare them (FAQ's) and then Email
them to me. I'll put them together in the general information file(s) which
are sent out whenever anyone asks for HELP.

The major reason for my concern here is when problems occur, users need
one address to send all problems to. If FAQ files are stored or used
as "reply" address then when problems occur an inappropriate individual
may get the messages and will likely not be able to take corrective actions.
This may sound trival, but remember the most frequent problems occur with
novices to the system. Those of you who are veterans know where to go and/or
who to contact. My basic principle has always been "Keep It Simple" so one
central site for things is best.


To get a copy of the current FAQ file, you need only send a message
to "LISTSERVER-at-AAEM.AMC.ANL.GOV" with the line SEND HELP or SEND FAQ.
As for weekly postings that's probably a bit much. Once a month
might be more reasonable. The main FAQ file is a bit too long to send out
monthly, I'll edit it down and use a shorter version as a monthly reminder.
That much I can easily automate.

Cheers....Nestor

Your Friendly Neighborhood SysOp.

P.S. And yes, please try to use appropriate subject lines. Everyone
will benefit. Any variation on 3, 4 or even 5 letter
abbreviations which is obvious will be fine. It will also
help in the archiving. Remember everything that has been
sent to this list is archived. It is still not searchable
as I need to develop a system for that, but Subject Titles
will help enormously.





From: Andy Gilicinski :      giliciag-at-ttown.apci.com
Date: Mon, 29 May 1995 17:18:54 -0400 (EDT)
Subject: AFM particles (reply)

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Henrik:

You should get good results with the larger particles in your range of
interest (100 nm and up) with Tempfix, per Don Chernoff's reply. You may
have some trouble with particles at the low end however - especially down
at 5 nm. Tempfix does have some topography of its own, and you will have
trouble distinguishing 5 nm particles from general topography of the
Tempfix. If the smallest particles "sink" at all into the Tempfix,
you'll also get an erroneous height measurement (if that's what you're
trying to get).

For the smallest particles, you'll probably need to use mica or something
else that can be made to be close to atomically flat. At that scale, if
you minimize the force enough (especially with tapping or non-contact mode
AFM) you should be ok w/r/to the particles being stable to imaging.

Good luck!

Andy

Dr. Andrew Gilicinski "opinions expressed are my own"
Analytical Technology Center
Air Products and Chemicals, Inc. (610) 481-5958 voice
Allentown, PA 18195 USA (610) 481-4600 fax

On Fri, 26 May 1995, Henrik Guldberg Pedersen wrote:

} To all AFM microscopists!
}
} I have had problems examining samples consisting small particles
} using the AFM. It seems like the particles are pushed around by the
} tip.
} Does anyone have any good ideas to solve this problem i.e. to hold
} the particles fixed - my small ceramic particles are usually 5 - 250
} nm in diameter.
} Any comment is appreciated.
}
} Kind Regards
} Henrik Guldberg Pedersen
} Institute of Mineral Industry
} Technical University of Denmark, bld. 204
} DK-2800 Lyngby
} Phone: + 45 45 25 22 47
} Fax: + 45 45 93 48 86
} E-Mail: imihgp-at-unidhp.uni-c.dk
}




From: STEPHAN HELFER :      S.Helfer-at-rbge.org.uk
Date: Tue, 30 May 1995 09:52:40 BST
Subject: Re: diamond knife

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To: "Dr. Molnar Peter" {MOLNARP-at-lib.dote.hu}

Peter
Were your fungi collected in the wild? I never section wild collected
material with the diamond unless it is absolutely required. I found too
many specimens had small sand particles attached to them and
would ruin the diamond. I use freshly broken glass knives (at 2 pence
apiece) for these specimens.

Yours sincerely


Dr Stephan Helfer, SSO
Mycologist

Royal Botanic Garden Edinburgh, Inverleith Row, EDINBURGH EH3 5LR,
Scotland UK

email s.helfer-at-rbge.org.uk
phone: +44 (0)131 552 7171 ext 280
or +44 (0)131 459 0446-280 (direct digital VoiceMail line)
fax: +44 (0)131 552 0382




From: Krzysztof Hubner :      zahubner-at-cyf-kr.edu.pl
Date: Tue, 30 May 1995 13:28:39 +0200 (METDST)
Subject: email adres Journal of Microscopy

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Dear Friends

Who know email adres Journal of Microscopy
(edited in London)

Thanks
Krzysztof Hubner

Foundry Research Institute
Krakow, Poland
{zahubner-at-cyf-kr.edu.pl}




From: y. thibault :      ythibaul-at-julian.uwo.ca
Date: Tue, 30 May 1995 09:23:35 -0400 (EDT)
Subject: EPMA , arsenopyrite standard

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I was just wondering if someone would know somewhere where i could find a
high quality (meaning very homogeneous) standard of arsenopyrite (FeAsS)
to use in a electron microprobe (EPMA)

Thanks,

Yves.

Yves Thibault
Dept. of Earth Sciences
University of Western Ontario
London, Ontario,
CANADA N6A 5B7





From: W.L. Steffens :      STEFFENS.B-at-calc.vet.uga.edu
Date: Tue, 30 May 1995 12:57:14 EST
Subject: Tissue culture embedment

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To John Gabrovsek,

I always recommend that cell cultures be grown in Leighton tubes
manufactured by Costar Plastics and distributed by Fisher. These tubes
have a detachable plastic coverslip that can be carried through processing
and embedment, then removed prior to sectioning, or sectioned along with
the embedment resin. This is by far the best method I have used for
sectioning monolayers.

-=W.L. Steffens=-
College of Veterinary Medicine
University of Georgia




From: naresh-at-pop.uky.edu (Naresh Shah)
Date: Tue, 30 May 1995 14:17:53 -0400
Subject: Re: EPMA , arsenopyrite standard

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} I was just wondering if someone would know somewhere where i could find a
} high quality (meaning very homogeneous) standard of arsenopyrite (FeAsS)
} to use in a electron microprobe (EPMA)
}
} Thanks,
}
} Yves.
}
} Yves Thibault
} Dept. of Earth Sciences
} University of Western Ontario
} London, Ontario,
} CANADA N6A 5B7
}
}
Have you tried Ward's? (800)962-2660
They have three grades available on pages 148,185 and 214 of their
1994-95 Earth Science catalog.

Naresh Shah
CFFLS, 341 Bowman Hall
University of Kentucky
Lexington, KY 40506-0059
(606)257-5119
(606)257-7215 FAX
Alternate e-mail: naresh-at-funky.cffls.uky.edu







From: Payton2
Date: Tue, Mar 28, 1995 11:10 PM EDT
Subject: Fwd: Chain letter

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LISTSEVER-at-aaem.amc.gov, SALLY.MAHONEY-at-asc.scottlan.edu


---------------------
Forwarded message:
Subj: Fwd: Chain letter


---------------------
Forwarded message:
Subj: Fwd: Chain letter


---------------------
Forwarded message:
Subj: Fwd: Chain letter


---------------------
Forwarded message:
Subj: Fwd: Chain letter


---------------------
Forwarded message:
Subj: Fwd: Chain letter


---------------------
Forwarded message:
Subj: Fwd: Chain letter


---------------------
Forwarded message:
Subj: Fwd: Chain letter


---------------------
Forwarded message:
Subj: Chain letter

This message has been sent to you for good luck. The original
is in New England. It has been sent around the world nine times. The
luck has now been sent to you. You will receive good luck within four
days of receiving this message - Provided you, in turn send it on.
This is no joke. You will receive good luck in your mailbox.
Send copies to people you think need good luck. Do not keep this
message.
This message must leave your hands in 96 hours.

A United States Air Force Officer received 470,000 Dollars.
Another Man received 40,000 Dollars and lost it because he broke the
chain.
Whereas in the Philippines, Gene Welch lost his wife 51 days after
receiving the message. He failed to circulate the message. However,
before his death, he received 7,555,000 dollars.

Please send twenty copies and see what happen in four days.
The chain comes from Venezuela and has written by Saul De Groda,
A Missionary from South America. Since the copy must tour
the world, you must make twenty copies and send them to friends and
associates - After a few days you will get a surprise. This is true,
even if you are not superstitious.

Do note the following: Constantine Dias received this chain in 1958.
He asked his secretary to make twenty copies and send them out. A few
days later he won a lottery of two million dollars. Carlos Daditt, an
office employee, received the message and forgot that it had to leave
his
hands in 96 hours.He lost his job. Later, after finding that message
again,
He mailed twenty copies. A few days later he got a better job.

Dalan Fairchild received the message and, not believing - Threw the
message away. Nine days later he died. In 1987, The message received by
a young woman in California was very faded and barely readable. She
promised herself that she would retype the message and send it on, But
she set it aside to do it later. She was plagued with various problems,
including expensive car repairs. The letter did not leave her hands
within
96 hours. She finally typed the letter as promised and got a new car.

Good Luck but please remember: 20 copies of this message must leave
your hands in 96 hours... You must not sign on this message...

--------------
Forwarded Message:


To: ASCOT LOCK, Josh Smith, JAnn Smith, Dev Smith, Josh487, Al Smith,
Smith Bear, IowaSis, SamRy, SMITH AMY, Mott Smith, HannaSmith, JASSMITH,
Rudley, Smith WA, Xcgirl, Lis Smith, Cap Scott, Scottkins, ScottT NYC

--------------
Forwarded Message:


To: Chris
cc: LiNdA, Larry, Laura, Michael, SHousley, LLCogdell, JLEnterpr, Laura
KAO, Laura N134, Deb 1063, Laura C F, Laura A H, GMINSD, Laura 512, LAURA HU,
Laura Bloo, CHEF LAURA, John, EFX Master, LisaYOG

--------------
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To: AmandaC117, RenitaLL, Mpetemoss, Osuwrestl, JERKYBOY27, JAKimble,
Member7995, Drantie, PPiris, Sarah72981, BECKY121, ByzIIFan, GrandAmlvr,
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To: SNOWTIG
cc: Grrrl99, Peasbi, Kara L Tho, IAmNot4U, ERGARD, Skibuny107, TONI A
32, B4Ball, Beth476, Alana618, MEGLN, BARBIE 169, DRD33, Jfezell, Weence,
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cc: BCEMT91b, BGBARB, BklynBoy69, Elfcandle, EparagoN, Crmsnprnce, ILE
MEK, Hot69Rod, Javier237, Jessy ny, LindaB19, MMcke, MRobin7728, PecGuy,
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To: Beetle16

--------------
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To: BB Dianne, BullBoat, Jenn0024, Beene1, CORIN, Brooke1209,
JOlson8925, Vmplv, Jene1959, JESSFLA19, Playsoc316, FNicolosi, Axandra,
Rubie23, Blackdress, Llyr, CATWOMAN17, HAPPY369, HLB810, Pickygrl, MERFIE101

--------------
Forwarded Message:


To: DWatson782

--------------
Forwarded Message:


here


--------------
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To: Sellback, EGW, Calgal14, Wgaynor, GLO, SGlah, L Lunachic, Gemlover,
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To: Bob
cc: RROZZONI, Chrismog, JAC 6688, Dr Dogg, Oreochild, Tim, GNelson770,
Dr Gamewiz, Lemon, Dekion1, JoelC01, CyanZero, Larson-at-prodigy.com, J centaur,
Buuster, FROGLUVER, Bill, Skater 1, Sellback

--------------
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To: SteelDaddy
cc: Kazin, MogDecker1, Pumpkin288, LemonLemon, MikeEGray, Omega47,
Sethron, Greensmith, Tre1216, Amon2628, Chocobop, Zed964, Dekion1,
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To: Jay630

--------------
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cc: Mandabarb, MikeRansom, Kestri, Maize69, NuttyPNut, Blonddie, L
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To: Ander2099, Sky Ryder3, Delphynus, Miss Imina, Tatiyanna, JT Lion,
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Yeyinde, ChewbaccAS, Aquilla, DrgnQueen7, Drythyn, LBite, Tig Irb

--------------
Forwarded Message:


To: Kefka100

--------------
Forwarded Message:


To: Librogue, Jon1Carlo, Smutsuqi, MizBird, Pinky1000, NLubin, SonorL,
idweinberg-at-ucdavis.edu

--------------
Forwarded Message:


To: Jon1Carlo, Smutsuqi, NLubin, DBERLAN, BERLAN, K co Cool, SonorL,
DanielBarc, Ackbar94, RSQUADRA, KBrewer-at-teetot.acusd.edu,
EBrewer-at-chaph.usc.edu, VDMF56A-at-prodigy.com, Moten, EL GUIDO, ABJZ, Happyfoot,
DrewBald, SNKay, GymShoes

--------------
Forwarded Message:


To: STUSSY, K co Cool, RSQUADRA, Rebecca674, Tennis121, EBecker789,
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Tickler, Jymbe, Kimmiebaby, Guide MST, Guide QST, KO Sara, KO Solar

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To: LilMac6887, Peterfin, B Berreth, RS Judy XC, Ranma11

--------------
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Send it on


--------------
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Here read this


--------------
Forwarded Message:


To: EAEY13A-at-prodigy.com, CARRIE3656, Devster597, Mariah P, DewDewHead,
NgtvCreep1, NightieRC, ZRT900, SeppL, RAJP-at-vm.temple.edu, TQuill2, S MiLAN,
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Spiderella, Wolfinator, Mav888, DocMP, TonyP1414, ERBrotman, IS 227,
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--------------
Forwarded Message:


To: DevinP

--------------
Forwarded Message:


To: Alyndag

--------------
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To: KRowan1048

--------------
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To: PACK 15

--------------
Forwarded Message:


To: JuanjA5463, PBGemini, Crystal914, Thrive95, RGSKMA, GROOVY DOG,
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--------------
Forwarded Message:


To: Srotag22

--------------
Forwarded Message:


.


--------------
Forwarded Message:


cc: Phalanx123, Punk core, Lisashot

here


--------------
Forwarded Message:


To: WarGymnast

--------------
Forwarded Message:


To: Kitt4

--------------
Forwarded Message:


This message has been sent to you for good luck. The original
is in New England. It has been sent around the world nine times. The
luck has now been sent to you. You will receive good luck within four
days of receiving this message - Provided you, in turn send it on.
This is no joke. You will receive good luck in your mailbox.
Send copies to people you think need good luck. Do not keep this
message.
This message must leave your hands in 96 hours.

A United States Air Force Officer received 470,000 Dollars.
Another Man received 40,000 Dollars and lost it because he broke the
chain.
Whereas in the Philippines, Gene Welch lost his wife 51 days after
receiving the message. He failed to circulate the message. However,
before his death, he received 7,555,000 dollars.

Please send twenty copies and see what happen in four days.
The chain comes from Venezuela and has written by Saul De Groda,
A Missionary from South America. Since the copy must tour
the world, you must make twenty copies and send them to friends and
associates - After a few days you will get a surprise. This is true,
even if you are not superstitious.

Do note the following: Constantine Dias received this chain in 1958.
He asked his secretary to make twenty copies and send them out. A few
days later he won a lottery of two million dollars. Carlos Daditt, an
office employee, received the message and forgot that it had to leave
his
hands in 96 hours.He lost his job. Later, after finding that message
again,
He mailed twenty copies. A few days later he got a better job.

Dalan Fairchild received the message and, not believing - Threw the
message away. Nine days later he died. In 1987, The message received by
a young woman in California was very faded and barely readable. She
promised herself that she would retype the message and send it on, But
she set it aside to do it later. She was plagued with various problems,
including expensive car repairs. The letter did not leave her hands
within
96 hours. She finally typed the letter as promised and got a new car.

Good Luck but please remember: 20 copies of this message




From: william d.Riley :      72650.1636-at-compuserve.com
Date: 31 May 95 00:32:33 EDT
Subject: Chain Letter

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Ladies and or gentleman: PULLeze!! keep such nonsense off this mailing list. I
believe that most of us don't want to hear it.Nestor? you concur?





From: dietmar.reiter-at-uibk.ac.at (Dietmar Reiter)
Date: Wed, 31 May 1995 08:35:04 +0100
Subject: Re: email adres Journal of Microscopy

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X-Sender: c71956-at-u1.uibk.ac.at
Message-Id: {v01510100abf1c96e8e3b-at-[138.232.71.39]}
Mime-Version: 1.0
Content-Type: text/plain; charset=us-ascii

} Who know email adres Journal of Microscopy
} (edited in London)

There is no one right now - as far as I know. But you might try the
anonymous ftp server of the ROYAL MICROSCOPY SOCIETY at:

IP: 163.1.59.101
User: rms
password: rms

Fax: +44-865-791237
phone: +44-865-248768

Hope this helps -Dietmar-

:-)

+++ Dietmar Reiter, Dept. of Zoology and Limnology
+++ University of Innsbruck
+++ Technikerstrasse 25, A-6020 Innsbruck, Austria
+++ phone: (43)-512-507 ext. -6170, fax ext. -2930






From: dietmar.reiter-at-uibk.ac.at (Dietmar Reiter)
Date: Wed, 31 May 1995 09:06:43 +0100 (BST)
Subject: Re: email adres Journal of Microscopy

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Message-Id: {199505310806.JAA16685-at-zeus.bris.ac.uk}

} } Who know email adres Journal of Microscopy
} } (edited in London)
}
} There is no one right now - as far as I know. But you might try the
} anonymous ftp server of the ROYAL MICROSCOPY SOCIETY at:
}
} +++ Dietmar Reiter, Dept. of Zoology and Limnology
}
The Royal Microscopical Society (publishers of the Journal of Microscopy)
has an e-mail address, for its home in Oxford:

rms-at-vax.ox.ac.uk

--
Keith R. Hallam
Research Associate

University of Bristol, |
Interface Analysis Centre, | Telephone: National (0117) 925 5666
Oldbury House, | International + 44 117 925 5666
121, St. Michael's Hill, | Facsimile: National (0117) 925 5646
Bristol, | International + 44 117 925 5646
BS2 8BS, | E-mail: k.r.hallam-at-bristol.ac.uk
England | URL: http://zeus.bris.ac.uk/~phkrh/




From: jester-at-crnjjsgi.swmed.edu (James V. Jester)
Date: Wed, 31 May 1995 09:11:27 -0500
Subject: Post Doctoral Position

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Applications are invited for a full-time position as
a Research Associate in the Department of Ophthalmology
at the University of Texas Southwestern Medical Center at Dallas.
We are seeking an individual with interest and experience in
microscopy and computer imaging. The ideal candidate will have
a Ph.D. in Biomedical Engineering, Cell Biology or related discipline;
although applicants with an M.S. degree and equivalent experience will
also be considered. The proposed work will focus on the quantitation
of 3- and 4-dimensional morphologic data obtained from patients and
experimental tissue, using confocal microscopy. Responsibilities
will include digitizing, processing, interpreting and analyzing
images, in order to generate reliable quantitative data. Applicants
should send a curriculum vitae with statement of interest to:

Dr. W. Matthew Petroll
Department of Ophthalmology
University of Texas Southwestern Medical Center at Dallas
5323 Harry Hines Blvd.
Dallas, TX 75235-9057

FAX: 214-648-2382
EMAIL: petroll-at-crnmpsgi.swmed.edu


UT Southwestern is an equal opportunity employer.





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Wed, 31 May 1995 9:03:11 -0500 (CDT)
Subject: Yes I saw the Chain Letter!!!!

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Subscribers,

Yes I saw the chain letter. I have contacted AOL to
complain about the user. Please donot post any comments
about it on the List. If you want just send back
an angry letter to the TWIT that did it. The address is:

R100186-at-aol.com

However, I have no way of finding out who this was a the
moment. I' will be waiting for a reply from AOL.

Also this person is NOT a subscriber to the Listserver,


Sorry...... Nestor




From: PRC-at-bragg.bio.purdue.edu
Date: Wed, 31 May 1995 11:17:09 -0500 (EST)
Subject: HRTEM calibration standard

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Message-Id: {m0sGpLr-0001yRC-at-pegasus.cc.ucf.edu}

Hi all,
Does anyone know of a supplier where we can purchase partially
graphitized carbon in suspension. Please no messages regarding the
pre-mounted variety of grids available as we would prefer to make our
own test grids.
Paul Chipman
Purdue University
Lilly Hall of Life Sciences
West Lafayette, In, 47907
PRC-at-bragg.bio.purdue.edu




From: Systems For Research Corp. :      sy4rsrch-at-dragon.achilles.net
Date: Wed, 31 May 1995 12:50:33 -0400 (EDT)
Subject: SPM- AFM Workshop & Samples Run at MSC

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Systems for Research is offering two services for
MSC attendees. If interested, you may attend either our:
{ A) AFM workshop (June 7th)
{ B) And/or have your samples run on Digital Instrument's
Nanoscope IIIa Atomic Force Microscope (June5,6,7th)
Both of these services will be offered at the MSC Meeting
held in Ottawa, June 5-7th.

AFM Workshop Details:
Time: 8:30-10:30 am, June7th
Cost: No charge
Enrollment: Limited to 20 individuals
Having Your Samples Run- Details:
Time:-June 5,6,7th
Cost: No charge
Enrollment: Please contact us before June 2nd.
Others may sign up at MSC - space permitting

--------------------------

Art Priebe- Systems For Research Corp
{ sy4rsrch-at-dragon.achilles.net
Phone (613)832-0094 fAx (613)832-4102




From: Stanley L Flegler :      flegler-at-pilot.msu.edu
Date: Wed, 31 May 1995 13:33:13 -0400 (EDT)
Subject: Need infrared microscopy info

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Message-Id: {9505311733.AA46080-at-pilot1.cl.msu.edu}

Is anyone on the listserver actively involved in infrared microscopy? I am
looking for someone who has practical experience with the technique as well as
some recent review articles. Stan Flegler, Center for Electron Optics,
Michigan State University, flegler-at-pulot.msu.edu




From: Gail J Celio :      celiogai-at-student.msu.edu
Date: Wed, 31 May 1995 16:48:04 -0400 (EDT)
Subject: SEM: Glutaraldehyde as fixative

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Message-Id: {9505312048.AA167841-at-student1.cl.msu.edu}

I've been trying to find a fixative procedure to use on powdery mildew of
poinsettias, and my recipe-searching has led me to glutaraldehyde solutions.
I want to try out some protcols I've found in other papers, but the glut. we
have in our lab is Grade II, 25% aq. soln. (Sigma) and is labeled as "not
recommended for use as an Electron microscopy fixative", citing the presence
of an interfering impurity, possibly a polymer, that can be detected at 235
nm. My question is, does this concern only apply to TEM, and the glut. can
be used for SEM work, or should I purchase new glut. with Grade I quality?
Also, if you have any favorite powdery mildew fixing procedures for the SEM,
I'd be open to suggestions.

Thanks,
--Gail Celio




From: SGKCCK-at-aol.com
Date: Wed, 31 May 1995 18:58:47 -0400
Subject: GLUTARALDEHYDE AS A FIXATIVE FOR

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I saw your question regarding the glut. It is important the purity of the
glutaraldehyde that you use as a fixative not only in TEM but in SEM as well.
When there are peaks at 235 your glut is "dirty" and may cause you
difficulty in your work. I would highly reccommend for a simple !0.00 you
but EM grade glut where you will be assured the highest purity and obtain the
best results from your fixative. If you have any questions or I can be of
any assistance to you please do not hesitate to contact me at Electron
Microscopy Sciences.
I look forward to hearing from you.
Sincerely,
Stacie Kirsch
Electron Microscopy Sciences
P.o.Box 251
Fort Washington, Pa 19034
Tel: 215-646-1566
Fax: 215-646-8931




From: Tony McKenna - NZ Dairy Research Institute :      MCKENNA-at-ECCLES.NZDRI.Org.NZ
Date: 01 Jun 1995 14:06:32 +1200
Subject: Help

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Help to subscribe





From: GVKM07A-at-prodigy.com (DR CHARLES A GARBER)
Date: Thu, 01 Jun 1995 00:31:45 EDT
Subject: SEM Glut as fixative

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-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --

On May 31, Gail Celio asked about glutaraldehyde purity.

Glutaraldehyde tends to be sold as two different "qualities", one being
in the "jugs" or "bottles" which is given various descriptions, such as
"technical" grade or "biological" grade and the other, in the sealed
glass ampoules described generally as "EM grade". Only one or maybe
two firms really do "manufacture" glutaraldehyde one being Union
Carbide and (I think also) Rohm & Haas. The "value added" by firms
like SPI Supplies (and of course others) is to "purify" the as received
material that comes out of the plants, which is "technical grade", and
which contains dimers and trimers, etc. of the monomer, into a form
that has sufficient purity for use in EM applications. To maintain the
purity and to give the product long shelf life, the EM grade product is
packaged in sealed glass ampoules under a dry nitrogen atmosphere.

The higher the starting purity, the longer the shelf life. SPI
Supplies guarantees its EM grade glut for one year unrefrigerated to be
free of the impurity peak.

So just what does constitute "EM quality" from the other (e.g. lesser)
quality? It relates to a purification process by which the glut is
refined and the dimers and trimers are removed (e.g. the source of the
impurity peak seen with UV/VIS spectrometry). You should be using for
your kind of work the "EM grade" glut that comes in the sealed glass
ampoules.

So although (I think) most firms offering the glut in jugs or bottles
do purify it down to that point before bottling, the shelf life
stability is so uncertain and uncontrollable, exacting researchers tend
to shy away from that particular form of packaging. Now this
uncertainty is not something unique to the brand you have on hand, it
would be just as true with our own SPI-Chem "Biological" grade glut,
for example.

So you very definitely should be using the EM grade glut. A number of
firms, including SPI produce excellent EM grade glut in sealed glass
ampules. So long as the product has been refined down to the point
that the impurity peak is not present, I don't believe one can say
that one glut is "better" than some other, given the same UV/VIS
spectrum. But there can (apparently) be differences in the shelf life.

So we are talking about a product that is so inexpensive, there should
not be any question about discarding the material you have on hand and
procuring some ampoules of EM grade glut, which is available in
concentrations of 8,25,50 and 70% choices, 10 ml per ampoule.

Hope this helps out in some small way.

Charles A. Garber, Ph. D.
President
SPI Supplies
PO Box 656
West Chester, PA 19381-0656 USA

Ph: (800) 2424-SPI [Toll free from USA]
(800) 526-6562 [Toll free from Canada]

FAX:(610) 436-5755

e-mail: GVKM07A-at-prodigy.com





From: STEPHAN HELFER :      S.Helfer-at-rbge.org.uk
Date: Thu, 1 Jun 1995 09:39:03 BST
Subject: Re: SEM: Glutaraldehyde as fixative

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To: Gail J Celio {celiogai-at-student.msu.edu}

Dear Gail

Having observed powdery mildews in the SEM for considerable time I
have come to three most satisfactory techniques, depending on
whether you would like to observe the conidia or the hyphae on the
leaf surface (in which case the conidia are only in the way and prone
to charging).

1) Obviously the Electroscan SEM would be the ideal instrument for
these observations.

2) Do you have access to CRYO SEM? If not don't dispair; my
favourite technique is to mount fresh specimens (NOT dripping wet!)
onto the stubs and observe them straight in the SEM. You must check
with your technician if this is acceptable ('cause the vacuum pump oil
might get contaminated, however, we found with a turbo pump that
this is not a problem, as long as the procedure isn't done too often
and the backing pump becomes contaminated too quickly).

I normally use a low accelerating voltage (1-5kV) and a short WD with
a reasonably small spot. Using this technique you may observe your
specimens for around 20min before they collaps. The surface
moisture is sufficiently conductive to avert charging and you can go
up to X10,000 without problem.

3) Alternatively, and if the above is unaccepable, I have used EM
grade GA and the standard CPD techniques. You have to be aware,
however, that most of your conidial chains will be disturbed using
chemical fixation (as in fact they will be if you plunge specimens into
liquid nitrogen for CRYO work), and the leaf surface waxes will also
be partially dissolved.

If you are interested I shall email you an image of Rhododendron
powdery mildew generated using option 2) technique above.

Yours sincerely


Dr Stephan Helfer, SSO
Mycologist

Royal Botanic Garden Edinburgh, Inverleith Row, EDINBURGH EH3 5LR,
Scotland UK

email s.helfer-at-rbge.org.uk
phone: +44 (0)131 552 7171 ext 280
or +44 (0)131 459 0446-280 (direct digital VoiceMail line)
fax: +44 (0)131 552 0382




From: Jan Coetzee EM Univ Pretoria :      JANC-at-ccnet.up.ac.za
Date: Thu, 1 Jun 1995 11:23:39 GMT+2
Subject: Re: SEM - powdery mildew

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Regarding the thread on the preparation of delicate fungal structures -

Another possible preparation method that often gives very acceptable
results is Osmium vapour fixation:

Pieces of leaf (or agar) with attached fungal structures are exposed to
the vapours from osmium tetroxide. This is easily done by leaving the
specimens in a closed container (tape sealed petri dish) in the presence of a
few drops of 1 percent aqueous OsO4. Remove the specimens after a day, dry in
a dessiccator over silica gel, mount and sputter coat.
OsO4 vapours are TOXIC, use the fume hood.

Jan Coetzee
Unit for Electron Microscopy Tel:+27-12-420-2075
University of Pretoria Fax:+27-12-342-1738
Pretoria Internet:janc-at-ccnet.up.ac.za
0002 South Africa




From: M. Choudhury :      mc142-at-cus.cam.ac.uk
Date: Thu, 1 Jun 1995 13:39:52 +0100 (BST)
Subject: UNSUBSCRIBE

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UNSUBSCRIBE




From: Joe Uknalis :      juknalis-at-arserrc.gov
Date: Thu, 01 Jun 1995 11:09:28 -0400 (EDT)
Subject: MSA

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MR-Received: by mta ISHTAR.MUAS; Relayed; Thu, 01 Jun 1995 11:09:28 -0400
MR-Received: by mta ISHTAR; Relayed; Thu, 01 Jun 1995 11:09:29 -0400
Disclose-recipients: prohibited

Sorry folks-
but could some kind soul tell me when/where MSA meeting is this year and
when
abstracts are due.
thanks

Joe

juknalis-at-arserrc.gov






From: sbianchi-at-dbag.unifi.it (Stefano Bianchi)
Date: Wed, 31 May 1995 09:21:44 +0100
Subject: Re: Chain Letter

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Microscopy {Microscopy-at-aaem.amc.anl.gov}
Message-id: {v01510101abf1d6b0ab34-at-[150.217.45.60]}
MIME-version: 1.0
Content-type: text/plain; charset="us-ascii"
Content-transfer-encoding: 7BIT

At 0:32 31-05-1995, william d.Riley wrote:
} Ladies and or gentleman: PULLeze!! keep such nonsense off this mailing list. I
} believe that most of us don't want to hear it.Nestor? you concur?
?






From: Dr P. Echlin :      pe13-at-cus.cam.ac.uk
Date: Thu, 1 Jun 1995 16:58:34 +0100 (BST)
Subject: Re: email adres Journal of Microscopy

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The Journal of Microscopy is published in Oxford by Blackwells Science on
behalf of the Royal Microscopical Society. You can iether contact the
Jornal Office by e-mail at :rms-at-vax.ox.ac.uk" or by phone at +44-1865
248768 or by fax +44-1865-791237.
You can contact me General Editor at "pe13-at-cus.cam.ac.uk" or by phone
+44-1223-333946 orfax +-44-1223-333953.
If all this modern and dispassionate forms of communication, it's always
a treat to receive a handwritten letter at Department of Plan Sciences,
University of Cambridge, Downing Street, Cambridge CB2 3EA UK.

Patrick Echlin
June ist 1995
University of Cambridge, Downing Street Cambridge BB2 3EA UK.On Tue, 30 May
1995, Krzysztof Hubner wrote:

}
} Dear Friends
}
} Who know email adres Journal of Microscopy
} (edited in London)
}
} Thanks
} Krzysztof Hubner
}
} Foundry Research Institute
} Krakow, Poland
} {zahubner-at-cyf-kr.edu.pl}
}




From: jeanne_barker-at-merck.com (Jeanne Barker)
Date: 1 Jun 1995 12:33:53 U
Subject: Methyl cellulose solution

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Message-Id: {9506011647.AA08823-at-igw.merck.com}

REGARDING Methyl cellulose solution
Hi,
What is the best way to make a 2% aqueous solution of methyl cellulose?
Should the water be cold, hot or RT? Does it require overnight spinning?
Thanks in advances for your responses.
Jeanne






From: M.V. Parthasarathy :      mvp2-at-cornell.edu
Date: Thu, 1 Jun 1995 12:31:55 -0400 (EDT)
Subject: RE: MSA

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X-NUPop-Charset: English

In message Thu, 01 Jun 1995 11:09:28 -0400 (EDT),
Joe Uknalis {juknalis-at-arserrc.gov} writes:

} Sorry folks-
} but could some kind soul tell me when/where MSA meeting is this year
} and when
} abstracts are due.
} thanks
}
} Joe
}
} juknalis-at-arserrc.gov
}
---------

Annual meeetings of MSA, MAS and Histochemical Society
will be held jointly at Kansas City Aug 13-17. MSA deadline for papers was
March 15th!

*************************************************************************
M.V. Parthasarathy
Professor of Plant Biology & Director, EM Facility
Section of Plant Biology
228 Plant Science Building
Cornell University, Ithaca, NY 14850
Telephone: (607) 255-1734
Fax: (607) 255-5407
E-mail: mvp2-at-cornell.edu
***********************************************************************




From: Goldmarker-at-aol.com
Date: Thu, 1 Jun 1995 13:43:02 -0400
Subject: Plastoid N..a source!

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Would anyone know a source for Plastoid N..an embedding media used for
demineralized bone.

A colleague is attempting to locate a new source for purchase.

Thank you.

Sincerely,

GOLDMARKER-at-AOL.COM




From: Goldmarker-at-aol.com
Date: Thu, 1 Jun 1995 13:43:05 -0400
Subject: Wrinkling acrylic resin..

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A colleague is having difficulty preventing wrinkling of acrylic resin (2-3u
thick) on glass slides. It does not seem to matter whether she uses
pll-coated, gelatin-coated, or uncoated slides.

She was told to add ethylene glycol to her formulation, but I am concerned
about modifying the vendor's formulation.

Does anyone have any suggestions?

Thanks.
Donald P. Cox, Ph.D. [goldmarker-at-aol.com]




From: Elinor Solit :      cambrex-at-world.std.com
Date: Thu, 1 Jun 1995 15:25:51 +0059 (EDT)
Subject: Re: MSA

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Joe,

MSA this year is in Kansas City. Abstract due date is a thing of the past.

For full information, suggest you contact Larry Maser or Sharon at the
MSA office, 800-538-3672.

See you in KC.

Ellie Solit
"The Microscope Book"

On Thu, 1 Jun 1995, Joe Uknalis wrote:

} Sorry folks-
} but could some kind soul tell me when/where MSA meeting is this year and
} when
} abstracts are due.
} thanks
}
} Joe
}
} juknalis-at-arserrc.gov
}
}




From: MAILER-DAEMON-at-emout04.mail.aol.com (Mail Delivery Subsystem)
Date: 95-06-01 17:19:07 EDT
Subject: Fwd: #2(2) Returned mail: Host unknown

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From: llsutter-at-mtu.edu
Date: 95-05-31 13:48:49 EDT
Subject: Fwd: #10(14) Chain Letter

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From: ard-at-atom.ansto.gov.au (Arthur Day)
Date: 95-05-31 12:32:36 EDT
Subject: Fwd: Re: Fwd: Chain letter

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Nice language Art Day
---------------------
Forwarded message:

WELL HERE'S MY 20 MESSAGES, FUCKWIT....




} ---------------------
} Forwarded message:
} Subj: Fwd: Chain letter
} Date: 95-05-28 20:16:21 EDT
} From: NJamie
} To: R100186
}
}
} ---------------------
} Forwarded message:
} Subj: Fwd: Chain letter
} Date: 95-05-28 13:17:11 EDT
} From: BZMomOf4
} To: NJamie
}
}
} ---------------------
} Forwarded message:
} Subj: Fwd: Chain letter
} Date: 95-05-28 13:08:57 EDT
} From: BZMomOf4
} To: JessZiG
}
}
} ---------------------
} Forwarded message:
} Subj: Fwd: Chain letter
} Date: 95-05-28 12:38:59 EDT
} From: STEVEJK192
} To: BZMomOf4
}
}
} ---------------------
} Forwarded message:
} Subj: Fwd: Chain letter
} Date: 95-05-22 15:57:01 EDT
} From: WHATZUP123
} To: STEVEJK192
}
}
} ---------------------
} Forwarded message:
} Subj: Fwd: Chain letter
} Date: 95-05-22 15:23:05 EDT
} From: Lsoccerja
} To: WHATZUP123
}
}
} ---------------------
} Forwarded message:
} Subj: Fwd: Chain letter
} Date: 95-05-18 17:43:25 EDT
} From: MPowell840
} To: Lsoccerja
}
}
} ---------------------
} Forwarded message:
} Subj: Chain letter
} Date: 95-05-16 13:55:21 EDT
} From: ALVikings
} To: MPowell840
}
} This message has been sent to you for good luck. The original
} is in New England. It has been sent around the world nine times. The
} luck has now been sent to you. You will receive good luck within four
} days of receiving this message - Provided you, in turn send it on.
} This is no joke. You will receive good luck in your mailbox.
} Send copies to people you think need good luck. Do not keep this
} message.
} This message must leave your hands in 96 hours.
}
} A United States Air Force Officer received 470,000 Dollars.
} Another Man received 40,000 Dollars and lost it because he broke the
} chain.
} Whereas in the Philippines, Gene Welch lost his wife 51 days after
} receiving the message. He failed to circulate the message. However,
} before his death, he received 7,555,000 dollars.
}
} Please send twenty copies and see what happen in four days.
} The chain comes from Venezuela and has written by Saul De Groda,
} A Missionary from South America. Since the copy must tour
} the world, you must make twenty copies and send them to friends and
} associates - After a few days you will get a surprise. This is true,
} even if you are not superstitious.
}
} Do note the following: Constantine Dias received this chain in 1958.
} He asked his secretary to make twenty copies and send them out. A few
} days later he won a lottery of two million dollars. Carlos Daditt, an
} office employee, received the message and forgot that it had to leave
} his
} hands in 96 hours.He lost his job. Later, after finding that message
} again,
} He mailed twenty copies. A few days later he got a better job.
}
} Dalan Fairchild received the message and, not believing - Threw the
} message away. Nine days later he died. In 1987, The message received by
} a young woman in California was very faded and barely readable. She
} promised herself that she would retype the message and send it on, But
} she set it aside to do it later. She was plagued with various problems,
} including expensive car repairs. The letter did not leave her hands
} within
} 96 hours. She finally typed the letter as promised and got a new car.
}
} Good Luck but please remember: 20 copies of this message must leave
} your hands in 96 hours... You must not sign on this message...
}
} --------------
} Forwarded Message:
}
} Date: Wed, May 3, 1995 10:58 PM EDT
} From: LAURA HU
} Subj: Fwd: Very Important
}
} To: ASCOT LOCK, Josh Smith, JAnn Smith, Dev Smith, Josh487, Al Smith,
} Smith Bear, IowaSis, SamRy, SMITH AMY, Mott Smith, HannaSmith, JASSMITH,
} Rudley, Smith WA, Xcgirl, Lis Smith, Cap Scott, Scottkins, ScottT NYC
}
} --------------
} Forwarded Message:
}
} Date: Wed, May 3, 1995 9:16 PM EDT
} From: ByzIIFan
} Subj: Fwd: Very Important
}
} To: Chris
} cc: LiNdA, Larry, Laura, Michael, SHousley, LLCogdell, JLEnterpr, Laura
} KAO, Laura N134, Deb 1063, Laura C F, Laura A H, GMINSD, Laura 512, LAURA
HU,
} Laura Bloo, CHEF LAURA, John, EFX Master, LisaYOG
}
} --------------
} Forwarded Message:
}
} Date: Tue, May 2, 1995 11:22 PM EDT
} From: SEXYLESLEY
} Subj: Fwd: Very Important
}
} To: AmandaC117, RenitaLL, Mpetemoss, Osuwrestl, JERKYBOY27, JAKimble,
} Member7995, Drantie, PPiris, Sarah72981, BECKY121, ByzIIFan, GrandAmlvr,
} Luckynclv, SirAiron, Wiltonier, Serri13, A VANDERPO, BryanAL, Mandie21
}
} --------------
} Forwarded Message:
}
} Date: Tue, May 2, 1995 10:15 PM EDT
} From: Javier237
} Subj: Fwd: Very Important
}
} To: SNOWTIG
} cc: Grrrl99, Peasbi, Kara L Tho, IAmNot4U, ERGARD, Skibuny107, TONI A
} 32, B4Ball, Beth476, Alana618, MEGLN, BARBIE 169, DRD33, Jfezell, Weence,
} SEXYLESLEY, MalCru, Jazmom
}
} --------------
} Forwarded Message:
}
} Date: Tue, May 2, 1995 9:56 PM EDT
} From: Beetle16
} Subj: Fwd: Very Important
}
}
} cc: BCEMT91b, BGBARB, BklynBoy69, Elfcandle, EparagoN, Crmsnprnce, ILE
} MEK, Hot69Rod, Javier237, Jessy ny, LindaB19, MMcke, MRobin7728, PecGuy,
} Piracy77, RSMALL333, Rw44, SMOKEDMAN, TexRob, Urtime
}
} --------------
} Forwarded Message:
}
} Date: Tue, May 2, 1995 2:15 AM EDT
} From: Brooke1209
} Subj: Fwd: Very Important
}
} To: Beetle16
}
} --------------
} Forwarded Message:
}
} Date: Sun, Apr 30, 1995 1:17 AM EDT
} From: DWatson782
} Subj: Fwd: Very Important
}
} To: BB Dianne, BullBoat, Jenn0024, Beene1, CORIN, Brooke1209,
} JOlson8925, Vmplv, Jene1959, JESSFLA19, Playsoc316, FNicolosi, Axandra,
} Rubie23, Blackdress, Llyr, CATWOMAN17, HAPPY369, HLB810, Pickygrl, MERFIE101
}
} --------------
} Forwarded Message:
}
} Date: Sat, Apr 29, 1995 2:05 AM EDT
} From: Biehler3
} Subj: Fwd: Very Important
}
} To: DWatson782
}
} --------------
} Forwarded Message:
}
} Date: Sat, Apr 29, 1995 1:20 AM EDT
} From: Dr Dogg
} Subj: Fwd: Very Important
} To: Eddie B1, Mortalmac2, TheShaka, Araya, BryanB2163, Druboy,
} JYHADMSTR, WILD GAMER, AL8726, OTEROFAM, Cypress69, JerryH7256, Misfit76,
JOE
} GAG2, JoeV455, TRENT69685, Biehler3, JohnnyMK3, CM3001, JodieWeiss
}
} here
}
}
} --------------
} Forwarded Message:
}
} Date: Thu, Apr 27, 1995 9:38 PM EDT
} From: GNelson770
} Subj: Fwd: Very Important
}
} To: Sellback, EGW, Calgal14, Wgaynor, GLO, SGlah, L Lunachic, Gemlover,
} Tink13577, Blueelena, Girly 16, Blond16, Andrea1234, Sage12, BlueEyed G,
} KCousin, DALAN L, Dr Dogg, SuprHST, Skater 1
}
} --------------
} Forwarded Message:
}
} Date: Thu, Apr 27, 1995 8:35 PM EDT
} From: Kevman3379
} Subj: Fwd: Very Important
}
} To: Bob
} cc: RROZZONI, Chrismog, JAC 6688, Dr Dogg, Oreochild, Tim, GNelson770,
} Dr Gamewiz, Lemon, Dekion1, JoelC01, CyanZero, Larson-at-prodigy.com, J
centaur,
} Buuster, FROGLUVER, Bill, Skater 1, Sellback
}
} --------------
} Forwarded Message:
}
} Date: Wed, Apr 26, 1995 11:48 PM EDT
} From: Jay630
} Subj: Fwd: Very Important
}
} To: SteelDaddy
} cc: Kazin, MogDecker1, Pumpkin288, LemonLemon, MikeEGray, Omega47,
} Sethron, Greensmith, Tre1216, Amon2628, Chocobop, Zed964, Dekion1,
} Kevman3379, Droopy999, AMSLAK, Dazed014, Ladisla, EnsnKim
}
} --------------
} Forwarded Message:
}
} Date: Wed, Apr 26, 1995 11:42 PM EDT
} From: Mandabarb
} Subj: Fwd: Very Important
}
} To: Jay630
}
} --------------
} Forwarded Message:
}
} Date: Wed, Apr 26, 1995 6:40 PM EDT
} From: JT Lion
} Subj: Fwd: Very Important
}
} To: Milava
} cc: Mandabarb, MikeRansom, Kestri, Maize69, NuttyPNut, Blonddie, L
} WILKIE, HardKorn, Ketep, DodgersMom, Mysitcal, SWAT MP5, Speclforcs,
} GillianJax, Xaynon, Jasmyne520, RangerBlue, Orc Master, LadyNimue2
}
} --------------
} Forwarded Message:
}
} Date: Wed, Apr 26, 1995 4:38 PM EDT
} From: Kefka100
} Subj: Fwd: Very Important
}
} To: Ander2099, Sky Ryder3, Delphynus, Miss Imina, Tatiyanna, JT Lion,
} L0rd Knot, Prince Rum, Cyric321, Timon 9, Simba Wi, Bilbo Adin,
VenomChaos,
} Yeyinde, ChewbaccAS, Aquilla, DrgnQueen7, Drythyn, LBite, Tig Irb
}
} --------------
} Forwarded Message:
}
} Date: Tue, Apr 25, 1995 7:35 PM EDT
} From: Jon1Carlo
} Subj: Fwd: Very Important
}
} To: Kefka100
}
} --------------
} Forwarded Message:
}
} Date: Mon, Apr 24, 1995 11:29 PM EDT
} From: Smutsuqi
} Subj: Fwd: Very Important
}
} To: Librogue, Jon1Carlo, Smutsuqi, MizBird, Pinky1000, NLubin, SonorL,
} idweinberg-at-ucdavis.edu
}
} --------------
} Forwarded Message:
}
} Date: Mon, Apr 24, 1995 8:06 PM EDT
} From: Pinky1000
} Subj: Fwd: Very Important
}
} To: Jon1Carlo, Smutsuqi, NLubin, DBERLAN, BERLAN, K co Cool, SonorL,
} DanielBarc, Ackbar94, RSQUADRA, KBrewer-at-teetot.acusd.edu,
} EBrewer-at-chaph.usc.edu, VDMF56A-at-prodigy.com, Moten, EL GUIDO, ABJZ,
Happyfoot,
} DrewBald, SNKay, GymShoes
}
} --------------
} Forwarded Message:
}
} Date: Sat, Apr 22, 1995 6:38 PM EDT
} From: RS Judy XC
} Subj: Fwd: Very Important
}
} To: STUSSY, K co Cool, RSQUADRA, Rebecca674, Tennis121, EBecker789,
} Pinky1000, PUGGY1000, Tails1235, WEETZIE11, LivWorden, FOOTICKLR1, DA
} Tickler, Jymbe, Kimmiebaby, Guide MST, Guide QST, KO Sara, KO Solar
}
} --------------
} Forwarded Message:
}
} Date: Sat, Apr 22, 1995 6:19 PM EDT
} From: Crickel
} Subj: Fwd: Very Important
}
} To: LilMac6887, Peterfin, B Berreth, RS Judy XC, Ranma11
}
} --------------
} Forwarded Message:
}
} Date: Mon, Apr 17, 1995 7:43 PM EDT
} From: Eagle516
} Subj: Fwd: Very Important
} To: DAISY11074, Convicts, Sandrasue, Sturf, Crickel, SairaK, ATUZ,
} Alirina, Trackar, Dolby1, Kambot3000, Flyme2nt, SeaDude911, JSCAP, LAMESHA,
} LisaB0069, ECK51, Swing35, Blkhunk, ROBOKELLEN, Eagle516, SMeld26705,
} Jimsatisfy
}
} Send it on
}
}
} --------------
} Forwarded Message:
}
} Date: Sun, Apr 16, 1995 7:34 PM EDT
} From: SeppL
} Subj: Fwd: Very Important
} To: JGFJr, NEZNARF 15, MacMan0000, Grishka, JFJD, Nirvana340, L Mabius,
} Rangers911, GeoScan, PG Miss, JessyEB, MarninS, LynxV, Groove2, ERIK A9131,
S
} MiLAN, Ardnaxelai, CalNet11, JudithMcK, CARRIE3656, Eagle516, NightieRC,
} ELMER321, MARCUSEE, CORY HUTCH, MaxatCap, JAbrams272, Chido, SimList2, AnjP,
} TQuill2
}
} Here read this
}
}
} --------------
} Forwarded Message:
}
} Date: Sat, Apr 15, 1995 2:50 PM EDT
} From: DevinP
} Subj: Fwd: Very Important
}
} To: EAEY13A-at-prodigy.com, CARRIE3656, Devster597, Mariah P, DewDewHead,
} NgtvCreep1, NightieRC, ZRT900, SeppL, RAJP-at-vm.temple.edu, TQuill2, S MiLAN,
} RuffAndy, GoldigR, Spfrantz, Hackmast, ELMER321, JHK RLK, Annice, Strs,
} Katers11, Missa100, Randilyn27, Pavlinka19, DanialH, StreetFunk, MGrivet,
} Spiderella, Wolfinator, Mav888, DocMP, TonyP1414, ERBrotman, IS 227,
} Goewyn1018, KAPP54, RENNEA, SkoolKat, Hotvettek
}
} --------------
} Forwarded Message:
}
} Date: Wed, Apr 12, 1995 10:20 PM EDT
} From: Alyndag
} Subj: Fwd: Very Important
}
} To: DevinP
}
} --------------
} Forwarded Message:
}
} Date: Mon, Apr 10, 1995 3:40 PM EDT
} From: KRowan1048
} Subj: Fwd: Very Important
}
} To: Alyndag
}
} --------------
} Forwarded Message:
}
} Date: Sun, Apr 9, 1995 5:11 AM EDT
} From: PACK 15
} Subj: Fwd: Very Important
}
} To: KRowan1048
}
} --------------
} Forwarded Message:
}
} Date: Sun, Apr 9, 1995 3:42 AM EDT
} From: GROOVY DOG
} Subj: Fwd: Very Important
}
} To: PACK 15
}
} --------------
} Forwarded Message:
}
} Date: Sat, Apr 8, 1995 3:28 AM EDT
} From: Srotag22
} Subj: Fwd: Very Important
}
} To: JuanjA5463, PBGemini, Crystal914, Thrive95, RGSKMA, GROOVY DOG,
} TLester726, V8thunder, ABOMB23, DncngBare, PJR29, EVEBIME, PsyburMan,
} AdamB51867, JWGASKINS, KitKelly, FRT, CyberLot, BryanOL, Ricky68
}
} --------------
} Forwarded Message:
}
} Date: Wed, Apr 5, 1995 9:49 PM EDT
} From: Yamahain
} Subj: Fwd: Very Important
}
} To: Srotag22
}
} --------------
} Forwarded Message:
}
} Date: Wed, Apr 5, 1995 8:15 PM EDT
} From: Punk core
} Subj: Fwd: Very Important
} To: Yamahain, Punker Joe, CleInd, Boyjer
}
} .
}
}
} --------------
} Forwarded Message:
}
} Date: Wed, Apr 5, 1995 3:15 PM EDT
} From: WarGymnast
} Subj: Fwd: Very Important
}
} cc: Phalanx123, Punk core, Lisashot
}
} here
}
}
} --------------
} Forwarded Message:
}
} Date: Tue, Apr 4, 1995 2:45 PM EDT
} From: Kitt4
} Subj: Fwd: Very Important
}
} To: WarGymnast
}
} --------------
} Forwarded Message:
}
} Date: Mon, Apr 3, 1995 6:33 PM EDT
} From: ATFRRT
} Subj: Fwd: Very Important
}
} To: Kitt4
}
} --------------
} Forwarded Message:
}
} Date: Tue, Mar 28, 1995 11:10 PM EDT
} From: Payton2
} Subj: Very Important
} To: ATFRRT
} cc: Someone
}
} This message has been sent to you for good luck. The original
} is in New England. It has been sent around the world nine times. The
} luck has now been sent to you. You will receive good luck within four
} days of receiving this message - Provided you, in turn send it on.
} This is no joke. You will receive good luck in your mailbox.
} Send copies to people you think need good luck. Do not keep this
} message.
} This message must leave your hands in 96 hours.
}
} A United States Air Force Officer received 470,000 Dollars.
} Another Man received 40,000 Dollars and lost it because he broke the
} chain.
} Whereas in the Philippines, Gene Welch lost his wife 51 days after
} receiving the message. He failed to circulate the message. However,
} before his death, he received 7,555,000 dollars.
}
} Please send twenty copies and see what happen in four days.
} The chain comes from Venezuela and has written by Saul De Groda,
} A Missionary from South America. Since the copy must tour
} the world, you must make twenty copies and send them to friends and
} associates - After a few days you will get a surprise. This is true,
} even if you are not superstitious.
}
} Do note the following: Constantine Dias received this chain in 1958.
} He asked his secretary to make twenty copies and send them out. A few
} days later he won a lottery of two million dollars. Carlos Daditt, an
} office employee, received the message and forgot that it had to leave
} his
} hands in 96 hours.He lost his job. Later, after finding that message
} again,
} He mailed twenty copies. A few days later he got a better job.
}
} Dalan Fairchild received the message and, not believing - Threw the
} message away. Nine days later he died. In 1987, The message received by
} a young woman in California was very faded and barely readable. She
} promised herself that she would retype the message and send it on, But
} she set it aside to do it later. She was plagued with various problems,
} including expensive car repairs. The letter did not leave her hands
} within
} 96 hours. She finally typed the letter as promised and got a new car.
}
} Good Luck but please remember: 20 copies of this message







From: hukee.margaret-at-mayo.EDU (Marge Hukee)
Date: Thu, 1 Jun 1995 16:30:18 +0200
Subject: Wrinkling acrylic resin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Message-Id: {9506012128.AA27893-at-fermat.Mayo.EDU}
X-Sender: hukem-at-fermat.mayo.edu
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Dr. Cox

We float sections on a water bubble and expose the sections to vapor from
trichloroethylene for a few minutes prior to drying at 55 degrees C. This
usually will flatten sections if the resin has been cured sufficiently. If
you have any questions on this technique, feel free to e-mail.

Marge

Marge Hukee
EM Core Facility hukee.margaret-at-mayo.edu
Mayo Foundation (507) 284-3148
----------------------------------------------------------------------------
--






From: R100186-at-aol.com
Date: Thu, 1 Jun 1995 17:37:00 -0400
Subject: apology

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To every one who has not rec a personal apology here it is .I"AM SORRY it was
not intentional I have a new auto address program and (I'am not quite sure
how) it picked up your address and sent that letter. It will not happen
again.





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Thu, 1 Jun 1995 17:22:15 -0500 (CDT)
Subject: Trash Mail from R100186@aol.com

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Subscribers....

Yes I have seen the 2nd trash message. I have contacted
AOL (a second time) to complain about this user. If you wish to add
your complaint. You should address your message to:

Postmaster-at-AOL.COM

let them know you are complaining about the user

R100186-at-aol.com

If AOL sees enough complaints they should take some
action. Because this is an unmoderated list I donot see
the messages until after they have hit the network.
I apologize for this appearing on your screens, but
at the moment there is little I can do.

The best we can do is let AOL know that they have
an individual on their system that is acting in a manner
which is not compatible with a public forum (to say the least).

Nestor





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Thu, 1 Jun 1995 17:30:11 -0500 (CDT)
Subject: Trash Mail

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Subscribers....


I have send a second notice to AOL about their
user who is dumping junk mail onto the microscopy
listserver.

I notice that he has sent an apology, however, if
it happens again I will let you all know how to
contact AOL to complain. If they get several
thousand complaints then they may actually do
something.

Nestor....

If you do want to contact AOL the generic
address you should use is:

Postmaster-at-AOL.COM

Someone will see it....





From: m.dickson-at-unsw.edu.au (Melvyn R. Dickson)
Date: Fri, 2 Jun 1995 10:26:34
Subject: Micromaze foreline traps

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

To: microscopy-at-aaem.amc.anl.gov

I have been persuaded that micromaze traps are ideal for reducing
backstreaming into microscopes. BUT there are conflicting versions as to how
to run them. The manufacturer says to use them with a valve between the trap
and the (e.g.) microscope and bake the trap out with the valve closed once a
week (or so). I hear of others who just run the trap hot all the time and use
no valve.

Can we have some feedback on the best way to use Micromazes?
Valve?
No valve?
How does it trap if its hot all the time?
Is a simmerstat needed to control operating temperature?

Thanks in advance.

Mel dickson.




From: anncali-at-andromeda.rutgers.edu (Ann Cali)
Date: Thu, 01 Jun 1995 20:44:55 -0400
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
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Message-Id: {199506020059.UAA18492-at-andromeda.rutgers.edu}
X-Sender: anncali-at-andromeda.rutgers.edu
X-Mailer: Windows Eudora Version 2.0.3
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

please unsubscribe

Thank you
Ann Cali, Professor (anncali-at- andromeda.rutgers.edu)
Dept of Biological Sciences, Smith Hall
Rutgers University, Newark, NJ 07102
FAX=201-648-1007





From: anncali-at-andromeda.rutgers.edu (Ann Cali)
Date: Thu, 01 Jun 1995 21:04:49 -0400
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Message-Id: {199506020119.VAA18809-at-andromeda.rutgers.edu}
X-Sender: anncali-at-andromeda.rutgers.edu
X-Mailer: Windows Eudora Version 2.0.3
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"


Ann Cali, Professor (anncali-at- andromeda.rutgers.edu)
Dept of Biological Sciences, Smith Hall
Rutgers University, Newark, NJ 07102
FAX=201-648-1007





From: ard-at-atom.ansto.gov.au (Arthur Day)
Date: 95-05-31 03:27:24 EDT
Subject: Fwd: Re: Fwd: Chain letter

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Message-Id: {25060120323134-at-vms2.macc.wisc.edu}

Perhaps we are connected to a street gang.
---------------------
Forwarded message:

WELL HERE'S MY 20 MESSAGES, FUCKWIT....




} ---------------------
} Forwarded message:
} Subj: Fwd: Chain letter
} Date: 95-05-28 20:16:21 EDT
} From: NJamie
} To: R100186
}
}
} ---------------------
} Forwarded message:
} Subj: Fwd: Chain letter
} Date: 95-05-28 13:17:11 EDT
} From: BZMomOf4
} To: NJamie
}
}
} ---------------------
} Forwarded message:
} Subj: Fwd: Chain letter
} Date: 95-05-28 13:08:57 EDT
} From: BZMomOf4
} To: JessZiG
}
}
} ---------------------
} Forwarded message:
} Subj: Fwd: Chain letter
} Date: 95-05-28 12:38:59 EDT
} From: STEVEJK192
} To: BZMomOf4
}
}
} ---------------------
} Forwarded message:
} Subj: Fwd: Chain letter
} Date: 95-05-22 15:57:01 EDT
} From: WHATZUP123
} To: STEVEJK192
}
}
} ---------------------
} Forwarded message:
} Subj: Fwd: Chain letter
} Date: 95-05-22 15:23:05 EDT
} From: Lsoccerja
} To: WHATZUP123
}
}
} ---------------------
} Forwarded message:
} Subj: Fwd: Chain letter
} Date: 95-05-18 17:43:25 EDT
} From: MPowell840
} To: Lsoccerja
}
}
} ---------------------
} Forwarded message:
} Subj: Chain letter
} Date: 95-05-16 13:55:21 EDT
} From: ALVikings
} To: MPowell840
}
} This message has been sent to you for good luck. The original
} is in New England. It has been sent around the world nine times. The
} luck has now been sent to you. You will receive good luck within four
} days of receiving this message - Provided you, in turn send it on.
} This is no joke. You will receive good luck in your mailbox.
} Send copies to people you think need good luck. Do not keep this
} message.
} This message must leave your hands in 96 hours.
}
} A United States Air Force Officer received 470,000 Dollars.
} Another Man received 40,000 Dollars and lost it because he broke the
} chain.
} Whereas in the Philippines, Gene Welch lost his wife 51 days after
} receiving the message. He failed to circulate the message. However,
} before his death, he received 7,555,000 dollars.
}
} Please send twenty copies and see what happen in four days.
} The chain comes from Venezuela and has written by Saul De Groda,
} A Missionary from South America. Since the copy must tour
} the world, you must make twenty copies and send them to friends and
} associates - After a few days you will get a surprise. This is true,
} even if you are not superstitious.
}
} Do note the following: Constantine Dias received this chain in 1958.
} He asked his secretary to make twenty copies and send them out. A few
} days later he won a lottery of two million dollars. Carlos Daditt, an
} office employee, received the message and forgot that it had to leave
} his
} hands in 96 hours.He lost his job. Later, after finding that message
} again,
} He mailed twenty copies. A few days later he got a better job.
}
} Dalan Fairchild received the message and, not believing - Threw the
} message away. Nine days later he died. In 1987, The message received by
} a young woman in California was very faded and barely readable. She
} promised herself that she would retype the message and send it on, But
} she set it aside to do it later. She was plagued with various problems,
} including expensive car repairs. The letter did not leave her hands
} within
} 96 hours. She finally typed the letter as promised and got a new car.
}
} Good Luck but please remember: 20 copies of this message must leave
} your hands in 96 hours... You must not sign on this message...
}
} --------------
} Forwarded Message:
}
} Date: Wed, May 3, 1995 10:58 PM EDT
} From: LAURA HU
} Subj: Fwd: Very Important
}
} To: ASCOT LOCK, Josh Smith, JAnn Smith, Dev Smith, Josh487, Al Smith,
} Smith Bear, IowaSis, SamRy, SMITH AMY, Mott Smith, HannaSmith, JASSMITH,
} Rudley, Smith WA, Xcgirl, Lis Smith, Cap Scott, Scottkins, ScottT NYC
}
} --------------
} Forwarded Message:
}
} Date: Wed, May 3, 1995 9:16 PM EDT
} From: ByzIIFan
} Subj: Fwd: Very Important
}
} To: Chris
} cc: LiNdA, Larry, Laura, Michael, SHousley, LLCogdell, JLEnterpr, Laura
} KAO, Laura N134, Deb 1063, Laura C F, Laura A H, GMINSD, Laura 512, LAURA
HU,
} Laura Bloo, CHEF LAURA, John, EFX Master, LisaYOG
}
} --------------
} Forwarded Message:
}
} Date: Tue, May 2, 1995 11:22 PM EDT
} From: SEXYLESLEY
} Subj: Fwd: Very Important
}
} To: AmandaC117, RenitaLL, Mpetemoss, Osuwrestl, JERKYBOY27, JAKimble,
} Member7995, Drantie, PPiris, Sarah72981, BECKY121, ByzIIFan, GrandAmlvr,
} Luckynclv, SirAiron, Wiltonier, Serri13, A VANDERPO, BryanAL, Mandie21
}
} --------------
} Forwarded Message:
}
} Date: Tue, May 2, 1995 10:15 PM EDT
} From: Javier237
} Subj: Fwd: Very Important
}
} To: SNOWTIG
} cc: Grrrl99, Peasbi, Kara L Tho, IAmNot4U, ERGARD, Skibuny107, TONI A
} 32, B4Ball, Beth476, Alana618, MEGLN, BARBIE 169, DRD33, Jfezell, Weence,
} SEXYLESLEY, MalCru, Jazmom
}
} --------------
} Forwarded Message:
}
} Date: Tue, May 2, 1995 9:56 PM EDT
} From: Beetle16
} Subj: Fwd: Very Important
}
}
} cc: BCEMT91b, BGBARB, BklynBoy69, Elfcandle, EparagoN, Crmsnprnce, ILE
} MEK, Hot69Rod, Javier237, Jessy ny, LindaB19, MMcke, MRobin7728, PecGuy,
} Piracy77, RSMALL333, Rw44, SMOKEDMAN, TexRob, Urtime
}
} --------------
} Forwarded Message:
}
} Date: Tue, May 2, 1995 2:15 AM EDT
} From: Brooke1209
} Subj: Fwd: Very Important
}
} To: Beetle16
}
} --------------
} Forwarded Message:
}
} Date: Sun, Apr 30, 1995 1:17 AM EDT
} From: DWatson782
} Subj: Fwd: Very Important
}
} To: BB Dianne, BullBoat, Jenn0024, Beene1, CORIN, Brooke1209,
} JOlson8925, Vmplv, Jene1959, JESSFLA19, Playsoc316, FNicolosi, Axandra,
} Rubie23, Blackdress, Llyr, CATWOMAN17, HAPPY369, HLB810, Pickygrl, MERFIE101
}
} --------------
} Forwarded Message:
}
} Date: Sat, Apr 29, 1995 2:05 AM EDT
} From: Biehler3
} Subj: Fwd: Very Important
}
} To: DWatson782
}
} --------------
} Forwarded Message:
}
} Date: Sat, Apr 29, 1995 1:20 AM EDT
} From: Dr Dogg
} Subj: Fwd: Very Important
} To: Eddie B1, Mortalmac2, TheShaka, Araya, BryanB2163, Druboy,
} JYHADMSTR, WILD GAMER, AL8726, OTEROFAM, Cypress69, JerryH7256, Misfit76,
JOE
} GAG2, JoeV455, TRENT69685, Biehler3, JohnnyMK3, CM3001, JodieWeiss
}
} here
}
}
} --------------
} Forwarded Message:
}
} Date: Thu, Apr 27, 1995 9:38 PM EDT
} From: GNelson770
} Subj: Fwd: Very Important
}
} To: Sellback, EGW, Calgal14, Wgaynor, GLO, SGlah, L Lunachic, Gemlover,
} Tink13577, Blueelena, Girly 16, Blond16, Andrea1234, Sage12, BlueEyed G,
} KCousin, DALAN L, Dr Dogg, SuprHST, Skater 1
}
} --------------
} Forwarded Message:
}
} Date: Thu, Apr 27, 1995 8:35 PM EDT
} From: Kevman3379
} Subj: Fwd: Very Important
}
} To: Bob
} cc: RROZZONI, Chrismog, JAC 6688, Dr Dogg, Oreochild, Tim, GNelson770,
} Dr Gamewiz, Lemon, Dekion1, JoelC01, CyanZero, Larson-at-prodigy.com, J
centaur,
} Buuster, FROGLUVER, Bill, Skater 1, Sellback
}
} --------------
} Forwarded Message:
}
} Date: Wed, Apr 26, 1995 11:48 PM EDT
} From: Jay630
} Subj: Fwd: Very Important
}
} To: SteelDaddy
} cc: Kazin, MogDecker1, Pumpkin288, LemonLemon, MikeEGray, Omega47,
} Sethron, Greensmith, Tre1216, Amon2628, Chocobop, Zed964, Dekion1,
} Kevman3379, Droopy999, AMSLAK, Dazed014, Ladisla, EnsnKim
}
} --------------
} Forwarded Message:
}
} Date: Wed, Apr 26, 1995 11:42 PM EDT
} From: Mandabarb
} Subj: Fwd: Very Important
}
} To: Jay630
}
} --------------
} Forwarded Message:
}
} Date: Wed, Apr 26, 1995 6:40 PM EDT
} From: JT Lion
} Subj: Fwd: Very Important
}
} To: Milava
} cc: Mandabarb, MikeRansom, Kestri, Maize69, NuttyPNut, Blonddie, L
} WILKIE, HardKorn, Ketep, DodgersMom, Mysitcal, SWAT MP5, Speclforcs,
} GillianJax, Xaynon, Jasmyne520, RangerBlue, Orc Master, LadyNimue2
}
} --------------
} Forwarded Message:
}
} Date: Wed, Apr 26, 1995 4:38 PM EDT
} From: Kefka100
} Subj: Fwd: Very Important
}
} To: Ander2099, Sky Ryder3, Delphynus, Miss Imina, Tatiyanna, JT Lion,
} L0rd Knot, Prince Rum, Cyric321, Timon 9, Simba Wi, Bilbo Adin,
VenomChaos,
} Yeyinde, ChewbaccAS, Aquilla, DrgnQueen7, Drythyn, LBite, Tig Irb
}
} --------------
} Forwarded Message:
}
} Date: Tue, Apr 25, 1995 7:35 PM EDT
} From: Jon1Carlo
} Subj: Fwd: Very Important
}
} To: Kefka100
}
} --------------
} Forwarded Message:
}
} Date: Mon, Apr 24, 1995 11:29 PM EDT
} From: Smutsuqi
} Subj: Fwd: Very Important
}
} To: Librogue, Jon1Carlo, Smutsuqi, MizBird, Pinky1000, NLubin, SonorL,
} idweinberg-at-ucdavis.edu
}
} --------------
} Forwarded Message:
}
} Date: Mon, Apr 24, 1995 8:06 PM EDT
} From: Pinky1000
} Subj: Fwd: Very Important
}
} To: Jon1Carlo, Smutsuqi, NLubin, DBERLAN, BERLAN, K co Cool, SonorL,
} DanielBarc, Ackbar94, RSQUADRA, KBrewer-at-teetot.acusd.edu,
} EBrewer-at-chaph.usc.edu, VDMF56A-at-prodigy.com, Moten, EL GUIDO, ABJZ,
Happyfoot,
} DrewBald, SNKay, GymShoes
}
} --------------
} Forwarded Message:
}
} Date: Sat, Apr 22, 1995 6:38 PM EDT
} From: RS Judy XC
} Subj: Fwd: Very Important
}
} To: STUSSY, K co Cool, RSQUADRA, Rebecca674, Tennis121, EBecker789,
} Pinky1000, PUGGY1000, Tails1235, WEETZIE11, LivWorden, FOOTICKLR1, DA
} Tickler, Jymbe, Kimmiebaby, Guide MST, Guide QST, KO Sara, KO Solar
}
} --------------
} Forwarded Message:
}
} Date: Sat, Apr 22, 1995 6:19 PM EDT
} From: Crickel
} Subj: Fwd: Very Important
}
} To: LilMac6887, Peterfin, B Berreth, RS Judy XC, Ranma11
}
} --------------
} Forwarded Message:
}
} Date: Mon, Apr 17, 1995 7:43 PM EDT
} From: Eagle516
} Subj: Fwd: Very Important
} To: DAISY11074, Convicts, Sandrasue, Sturf, Crickel, SairaK, ATUZ,
} Alirina, Trackar, Dolby1, Kambot3000, Flyme2nt, SeaDude911, JSCAP, LAMESHA,
} LisaB0069, ECK51, Swing35, Blkhunk, ROBOKELLEN, Eagle516, SMeld26705,
} Jimsatisfy
}
} Send it on
}
}
} --------------
} Forwarded Message:
}
} Date: Sun, Apr 16, 1995 7:34 PM EDT
} From: SeppL
} Subj: Fwd: Very Important
} To: JGFJr, NEZNARF 15, MacMan0000, Grishka, JFJD, Nirvana340, L Mabius,
} Rangers911, GeoScan, PG Miss, JessyEB, MarninS, LynxV, Groove2, ERIK A9131,
S
} MiLAN, Ardnaxelai, CalNet11, JudithMcK, CARRIE3656, Eagle516, NightieRC,
} ELMER321, MARCUSEE, CORY HUTCH, MaxatCap, JAbrams272, Chido, SimList2, AnjP,
} TQuill2
}
} Here read this
}
}
} --------------
} Forwarded Message:
}
} Date: Sat, Apr 15, 1995 2:50 PM EDT
} From: DevinP
} Subj: Fwd: Very Important
}
} To: EAEY13A-at-prodigy.com, CARRIE3656, Devster597, Mariah P, DewDewHead,
} NgtvCreep1, NightieRC, ZRT900, SeppL, RAJP-at-vm.temple.edu, TQuill2, S MiLAN,
} RuffAndy, GoldigR, Spfrantz, Hackmast, ELMER321, JHK RLK, Annice, Strs,
} Katers11, Missa100, Randilyn27, Pavlinka19, DanialH, StreetFunk, MGrivet,
} Spiderella, Wolfinator, Mav888, DocMP, TonyP1414, ERBrotman, IS 227,
} Goewyn1018, KAPP54, RENNEA, SkoolKat, Hotvettek
}
} --------------
} Forwarded Message:
}
} Date: Wed, Apr 12, 1995 10:20 PM EDT
} From: Alyndag
} Subj: Fwd: Very Important
}
} To: DevinP
}
} --------------
} Forwarded Message:
}
} Date: Mon, Apr 10, 1995 3:40 PM EDT
} From: KRowan1048
} Subj: Fwd: Very Important
}
} To: Alyndag
}
} --------------
} Forwarded Message:
}
} Date: Sun, Apr 9, 1995 5:11 AM EDT
} From: PACK 15
} Subj: Fwd: Very Important
}
} To: KRowan1048
}
} --------------
} Forwarded Message:
}
} Date: Sun, Apr 9, 1995 3:42 AM EDT
} From: GROOVY DOG
} Subj: Fwd: Very Important
}
} To: PACK 15
}
} --------------
} Forwarded Message:
}
} Date: Sat, Apr 8, 1995 3:28 AM EDT
} From: Srotag22
} Subj: Fwd: Very Important
}
} To: JuanjA5463, PBGemini, Crystal914, Thrive95, RGSKMA, GROOVY DOG,
} TLester726, V8thunder, ABOMB23, DncngBare, PJR29, EVEBIME, PsyburMan,
} AdamB51867, JWGASKINS, KitKelly, FRT, CyberLot, BryanOL, Ricky68
}
} --------------
} Forwarded Message:
}
} Date: Wed, Apr 5, 1995 9:49 PM EDT
} From: Yamahain
} Subj: Fwd: Very Important
}
} To: Srotag22
}
} --------------
} Forwarded Message:
}
} Date: Wed, Apr 5, 1995 8:15 PM EDT
} From: Punk core
} Subj: Fwd: Very Important
} To: Yamahain, Punker Joe, CleInd, Boyjer
}
} .
}
}
} --------------
} Forwarded Message:
}
} Date: Wed, Apr 5, 1995 3:15 PM EDT
} From: WarGymnast
} Subj: Fwd: Very Important
}
} cc: Phalanx123, Punk core, Lisashot
}
} here
}
}
} --------------
} Forwarded Message:
}
} Date: Tue, Apr 4, 1995 2:45 PM EDT
} From: Kitt4
} Subj: Fwd: Very Important
}
} To: WarGymnast
}
} --------------
} Forwarded Message:
}
} Date: Mon, Apr 3, 1995 6:33 PM EDT
} From: ATFRRT
} Subj: Fwd: Very Important
}
} To: Kitt4
}
} --------------
} Forwarded Message:
}
} Date: Tue, Mar 28, 1995 11:10 PM EDT
} From: Payton2
} Subj: Very Important
} To: ATFRRT
} cc: Someone
}
} This message has been sent to you for good luck. The original
} is in New England. It has been sent around the world nine times. The
} luck has now been sent to you. You will receive good luck within four
} days of receiving this message - Provided you, in turn send it on.
} This is no joke. You will receive good luck in your mailbox.
} Send copies to people you think need good luck. Do not keep this
} message.
} This message must leave your hands in 96 hours.
}
} A United States Air Force Officer received 470,000 Dollars.
} Another Man received 40,000 Dollars and lost it because he broke the
} chain.
} Whereas in the Philippines, Gene Welch lost his wife 51 days after
} receiving the message. He failed to circulate the message. However,
} before his death, he received 7,555,000 dollars.
}
} Please send twenty copies and see what happen in four days.
} The chain comes from Venezuela and has written by Saul De Groda,
} A Missionary from South America. Since the copy must tour
} the world, you must make twenty copies and send them to friends and
} associates - After a few days you will get a surprise. This is true,
} even if you are not superstitious.
}
} Do note the following: Constantine Dias received this chain in 1958.
} He asked his secretary to make twenty copies and send them out. A few
} days later he won a lottery of two million dollars. Carlos Daditt, an
} office employee, received the message and forgot that it had to leave
} his
} hands in 96 hours.He lost his job. Later, after finding that message
} again,
} He mailed twenty copies. A few days later he got a better job.
}
} Dalan Fairchild received the message and, not believing - Threw the
} message away. Nine days later he died. In 1987, The message received by
} a young woman in California was very faded and barely readable. She
} promised herself that she would retype the message and send it on, But
} she set it aside to do it later. She was plagued with various problems,
} including expensive car repairs. The letter did not leave her hands
} within
} 96 hours. She finally typed the letter as promised and got a new car.
}
} Good Luck but please remember: 20 copies of this message







From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Thu, 1 Jun 1995 22:39:30 -0500 (CDT)
Subject: Ignore the Junk Mailer Please

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Hi folks,..

I know it is an irratiation, but please ignore
the junk mailer. I have independently contacted
AOL and asked that they do something.

For the time being DONOT send him back any return
junk mail, It only aggrevates the problem.

Thanks.... Nestor




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Fri, 2 Jun 1995 5:29:59 -0500 (CDT)
Subject: Listserver Status....

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From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Fri, 2 Jun 1995 5:29:18 -0500 (CDT)
Subject: Listserver Status

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Colleagues

In order to reduce the junk mail you are getting
and we get the nut off-line, I have switched the
listerserver into manual mode. This means I read
and then forward all messages. Everything will
still get through it will only take awhile longer
since I must physically screen each posting.

You should not change your methodology. Continue
to post to:

Microscopy-at-AAEM.AMC.ANL.GOV

Nestor




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Fri, 2 Jun 1995 5:34:56 -0500 (CDT)
Subject: Re: Wrinkling acrylic resin.

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From: Glen Macdonald :      glenmac-at-u.washington.edu
Date: Thu, 1 Jun 1995 23:01:19 -0700 (PDT)
Subject: Re: Wrinkling acrylic resin..

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X-Sender: glenmac-at-homer05.u.washington.edu

How were the slides cleaned? I've had problems that were traced back to
how well the slides were cleaned, even though the same subbing agent was
used. Chromic acid cleaned slides, subbed with gelatin are the only
thing that gets my Spurr's to lie flat when comverslipping with DPX. 50%
bleach, soaking slides 30 min., works almost as well, and also seems good
enough for AR (autoradiography, not the 'net definition). I put
methacrylate sections on bleach, chromic acid or slides cleaned with 1%
HCl in 95% ethanol, usually unsubbed, but also on chrome alum-gelatin or
pll subed slided.

Glen MacDonald
Hearing Development Laboratories
University of Washington
Seattle, WA 98195-3515
(206)543-8360
glenmac-at-u.washington.edu



On Thu, 1 Jun 1995 Goldmarker-at-aol.com wrote:

} A colleague is having difficulty preventing wrinkling of acrylic resin (2-3u
} thick) on glass slides. It does not seem to matter whether she uses
} pll-coated, gelatin-coated, or uncoated slides.
}
} She was told to add ethylene glycol to her formulation, but I am concerned
} about modifying the vendor's formulation.
}
} Does anyone have any suggestions?
}
} Thanks.
} Donald P. Cox, Ph.D. [goldmarker-at-aol.com]
}




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sun, 4 Jun 1995 10:10:18 -0500 (CDT)
Subject: RCA Photomultipliers

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From: Petr Schauer :      petr-at-ISIBrno.Cz
Date: Fri, 2 Jun 1995 16:13:40 +0200
Subject: RCA Photomultipliers

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Dear Networkers,

I am looking for a contact address (location, e-mail, fax, .....) of the
RCA company (photomultiplier tube division). Any help will be appreciated.

Petr
+-------------------------------------------+-------------------------+
| Dr. Petr Schauer | tel.: (+42 5) 41321246 |
| Head of Electron Microscopy Laboratory | fax : (+42 5) 746664 |
| ACADEMY OF SCIENCES OF THE CZECH REPUBLIC | (+42 5) 41211168 |
| INSTITUTE OF SCIENTIFIC INSTRUMENTS | E-mail: petr-at-isibrno.cz |
| Kralovopolska 147, CZ-612 64 Brno | petr-at-vabo.cz |
| Czech Republic | |
+-------------------------------------------+-------------------------+




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sun, 4 Jun 1995 10:11:06 -0500 (CDT)
Subject: XIVth Intnl Pfefferkorn Conference - update.

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Information on this meeting is also available at:
http://www.engin.umich.edu/~jfmjfm/mas_folder/XIVPfefferkorn.html

John Mansfield
North Campus Electron Microbeam Analysis Laboratory
413 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (313)936-3352 FAX (313)936-3352
jfmjfm-at-engin.umich.edu, John.F.Mansfield-at-umich.edu
or jfmjfm-at-umich.edu they all reach me!
URL: http://www.engin.umich.edu/~jfmjfm/jfmjfm.html






From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sun, 4 Jun 1995 10:12:31 -0500 (CDT)
Subject: Micromaze Foreline Traps

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From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sun, 4 Jun 1995 10:13:03 -0500 (CDT)
Subject: Advice re: 35mm slidemaker

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From: DCROMEY-at-CCIT.ARIZONA.EDU
Date: Fri, 02 Jun 1995 15:54:31 -0700 (MST)
Subject: Advice re: 35mm slidemaker

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We are trying to decide between a Polaroid HR-6000 and a LaserGraphics
LFR-X 35mm slidemaker. Does anyone out there have any feedback on which
is better (and why) and if you have experience with either unit, what
kind of things do you like/dislike about it?

Thanks for your help.
Doug

.....................................................................
. Douglas W. Cromey, M.S. Dept. of Cell Biology & Anatomy .
. Sr. Research Specialist University of Arizona .
. (office: LSN 451) 1501 N. Campbell Ave. .
. (voice: 520-626-2824) Tucson, AZ 85724 USA .
. (FAX: 520-626-2097) (email: dcromey-at-ccit.arizona.edu) .
.....................................................................





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sun, 4 Jun 1995 10:12:49 -0500 (CDT)
Subject: Micromaze Foreline Traps

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From: dlb-at-aruba.ccit.arizona.edu (David Bentley)
Date: Fri, 2 Jun 1995 14:14:05 -0700
Subject: Micromaze foreline traps

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The manfacturers suggested regeneration cycle is correct if I
understand your trap correctly. I am guessing that the trap is filled with
molecular sieve (zeolite) which adsorbs oil and water vapors when cool.
During the heating phase, these vapors are driven off. Without a valve in
the line there is a good chance that the oil vapor will migrate towards the
diffusion pump (i.e. into the microscope). A valve in the line will avoid
this and any vapor will be expelled by the rotary pump. After a period of
time the molecular seive will lose efficiency, which heating will regenerate
its adsorption capacity. It may also be benificial to gas ballast the
rotary pump during regeneration. This will insure that water vapor which
will also be driven off during heating, does not contaminate the rotary pump
oil as much.

A concise answer to your questions
Yes, place a valve on the line
Heat on a weekly basis
It won't trap if its hot all the time
I would assume that the manufacturer either:
Put a thermostat in with the heating element
-OR-
Has a time versus temperature curve that is used


David Bentley
later
dlb





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Sun, 4 Jun 1995 10:11:28 -0500 (CDT)
Subject: Position Available

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From: meh-at-aretha.jax.org (Margaret Hogan)
Date: Fri, 02 Jun 1995 12:07:32 -0400
Subject: Position Available

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Message-Id: {199506021610.MAA02376-at-aretha.jax.org}
X-Sender: meh-at-aretha.jax.org
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"


Histology - Biomedical Technologist/ Senior Biomedical Technologist

A regular full time position is open in The Jackson Laboratory Biological
Imaging Department - Histology Laboratory. The Jackson Laboratory is a
non-profit independent laboratory founded in 1929 on the premise that the
causes of cancer and other diseases could be discovered through
Mammalian genetic research. The Laboratory specializes in mammalian
genetics using inbred laboratory mice as model systems to study health
problems such as cancer diabetes, anemia, heart disease and aging.
Located on a large island in the gulf of Maine and surrounded by Acadia
National Park, The Jackson Laboratory is currently undergoing a major
expansion of its scientific staff and its research facilities.

Applicants with a Bachelors degree and two years related
laboratory experience working with Murine specimens preferred. The
position includes routine histological techniques ie paraffin embedding,
single and serial sectioning and cryotomy in conjunction with
Immunohistochemistry techniques, staining using heavy metals as well as
other special stains.

The successful candidate must be a self starter, pay attention to detail
and be able to work independently with little supervision.
This individual will be responsible for providing services in support of
numerous diverse research projects, must interact well with multiple
users and work productively in a team environment. The position includes
opportunities for advancement.

Salary range is mid to high $20,000 plus benefits and is negotiable
depending on level of experience.

Interested applicants should send CV to:

Joanne Bradt
Employment Specialist
The Jackson Laboratory
600 Main Street
Bar Harbor Maine 04609
(207) 288-3371 ext. 1281
(207) 288-3371 ext. 1082 FAX
jcb-at-aretha.jax.org





From: DCROMEY-at-CCIT.ARIZONA.EDU
Date: Fri, 02 Jun 1995 15:54:31 -0700 (MST)
Subject: Advice re: 35mm slidemaker

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Microscopy-at-AAEM.AMC.ANL.GOV, ZALUZEC-at-AAEM.AMC.ANL.GOV

Reply to: RE} Advice re: 35mm slidemaker
We have a Lazorgraphics LSR. The slides ore fine. However we
have had a problem with the shutter on the camera. One was
user or should I say miss-user problem. The second time the
shutter stoped opening in the middle of a role. It cost about $300
to get lazorgraphic to fix it the first time. (I am not sure how much a
new camera back would cost.) The second time was with in 90 days
of the first repair. Other than that any problems have been software
problem on our Mac or PC.
larry hawkey
hawkey-at-neuro.duke.edu

--------------------------------------


We are trying to decide between a Polaroid HR-6000 and a LaserGraphics
LFR-X 35mm slidemaker. Does anyone out there have any feedback on which
is better (and why) and if you have experience with either unit, what
kind of things do you like/dislike about it?

Thanks for your help.
Doug

.....................................................................
. Douglas W. Cromey, M.S. Dept. of Cell Biology & Anatomy .
. Sr. Research Specialist University of Arizona .
. (office: LSN 451) 1501 N. Campbell Ave. .
. (voice: 520-626-2824) Tucson, AZ 85724 USA .
. (FAX: 520-626-2097) (email: dcromey-at-ccit.arizona.edu) .
.....................................................................








From: DCROMEY-at-CCIT.ARIZONA.EDU
Date: Fri, 02 Jun 1995 15:54:31 -0700 (MST)
Subject: Advice re: 35mm slidemaker

Contents Retrieved from Microscopy Listserver Archives
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Message-Id: {199506051223.IAA14808-at-neuro.duke.edu}
Microscopy-at-AAEM.AMC.ANL.GOV, ZALUZEC-at-AAEM.AMC.ANL.GOV

Reply to: RE} Advice re: 35mm slidemaker
We have a Lazorgraphics LSR. The slides ore fine. However we
have had a problem with the shutter on the camera. One was
user or should I say miss-user problem. The second time the
shutter stoped opening in the middle of a role. It cost about $300
to get lazorgraphic to fix it the first time. (I am not sure how much a
new camera back would cost.) The second time was with in 90 days
of the first repair. Other than that any problems have been software
problem on our Mac or PC.
larry hawkey
hawkey-at-neuro.duke.edu

--------------------------------------


We are trying to decide between a Polaroid HR-6000 and a LaserGraphics
LFR-X 35mm slidemaker. Does anyone out there have any feedback on which
is better (and why) and if you have experience with either unit, what
kind of things do you like/dislike about it?

Thanks for your help.
Doug

.....................................................................
. Douglas W. Cromey, M.S. Dept. of Cell Biology & Anatomy .
. Sr. Research Specialist University of Arizona .
. (office: LSN 451) 1501 N. Campbell Ave. .
. (voice: 520-626-2824) Tucson, AZ 85724 USA .
. (FAX: 520-626-2097) (email: dcromey-at-ccit.arizona.edu) .
.....................................................................








From: A. Kent Christensen :      akc-at-umich.edu
Date: Mon, 5 Jun 1995 09:58:14 -0400 (EDT)
Subject: Re: Methyl cellulose solution

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Jeanne -- You can get this information in G. Griffith's "Fine Structure
Immunocytrochemistry", 1993, Springer-Verlag (Berlin, New York), ISBN
0-387-54805-X, pp. 175-177. -- A. Kent Christensen, Univ. of Michigan,
{akc-at-umich.edu} .

-----------------------------------

On 1 Jun 1995, Jeanne Barker wrote:

} REGARDING Methyl cellulose solution
} Hi,
} What is the best way to make a 2% aqueous solution of methyl cellulose?
} Should the water be cold, hot or RT? Does it require overnight spinning?
} Thanks in advances for your responses.
} Jeanne
}
}
}




From: DDKJoe-at-aol.com
Date: Mon, 5 Jun 1995 13:20:18 -0400
Subject: Re: Plastoid N..a source!

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Don,
Plastoid N has had on-again, off-again availability from the manufacturer in
Germany. At the moment, we have an inventory and a source. We would be
happy to help your colleague.

Joe Tabeling
Delaware Diamond Knives
3825 Lancaster Pike
Wilmington, DE 19805
800-222-5143




From: _ _ _ _ _ MARK W. LUND _ _ _ _ _ :      LUNDM-at-PHYSC2.BYU.EDU
Date: Mon, 5 Jun 1995 11:58 MDT
Subject: Re: RCA Photomultipliers.

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The RCA photomultplier company is now Burle Industries in
Lancaster PA. Tel: 717-296-6031
FAX 717-295-6096
regards
mark
---
Mark W. Lund, PhD
Director
MOXTEK, Inc.
Orem UT 84057




From: Strucural Biology Unit :      MICROSCOPY-at-SBSNOV1.AUCKLAND.AC.NZ
Date: Tue, 6 Jun 1995 17:16:24 GMT+1200
Subject: SEM versus TEM usage

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Two years ago our zoology, botany, cell biology and biochemistry
departments formed a school of biological sciences (SBS). It made
little difference to the EM unit as it already serviced the TEM needs
of those departments. SEM on the other hand is located in
engineering. We are considering the purchase of an SEM as the
engineering Philips 505 is becomming very limiting as it is set up
primarily for engineering applications.

Our problem is estimating likely demand for a new (probably
environmental) SEM. We are currently running at about
5% SEM to 95% TEM. We suspect this is due to the SEM being unsuitable
for many biological applications, and its position 500 metres up the
road. We would like feedback from other Schools of Biological
science with both SEM and TEM as to the proportion of use each gets.

Ken and Terry


Microscopy Unit
School Of Biological Sciences
The University Of Auckland
Level 1, Thomas Building
Private Bag 92019
Auckland, NEW ZEALAND

phone:(09) 373 7999 ext 5986
fax: (09) 373 7417




From: Keith Moulding :      MCMOULDK-at-usthk.ust.hk
Date: 06 Jun 1995 17:39:01 +0800
Subject: Software - MaComis

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Hi,

Does anyone know where MaComis can be obtained from? The
program simulates dislocation contrast.

Thanks in advance.

Keith Moulding.

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr. Keith Moulding, ~
Materials Characterisation and Preparation Centre, ~
Hong Kong University of Science and Technology, ~
Clear Water Bay, ~
Kowloon, ~
Hong Kong. ~
~
Tel: (852) 2358 8724 ~
Fax: (852) 2358 2451 ~
~
E-mail: mcmouldk-at-usthk.ust.hk ~
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~





From: {tealbe-at-ml.wpafb.af.mil}:ddn:wpafb
Date: 6-5-95 3:52pm
Subject: COMPUTER VIRUS ALERT

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Alternate-Recipient: allowed
Auto-Forwarded: prohibited
Content-Return: allowed
Disclose-Recipients: prohibited
Conversion: allowed
Importance: normal
Priority: normal
Sensitivity: Company-Confidential
Tribology ListServer {tribology-at-aaem.amc.anl.gov}
Message-Id: {950606082343.567-at-cliff.ml.wpafb.af.mil.0}
To: admin:ml:wpafb
Subj: COMPUTER VIRUS ALERT
Orig-Author: {Billy E. Teal {tealbe-at-ml.wpafb.af.mil} }:ddn:wpafb
-----------------------------------------------------------
COMPUTER VIRUS ALERT

Attention all DOS/Windows users. Someone is distributing
a file called PKZ300B.EXE or possibly PKZ300B.ZIP. This
is NOT a version of PKZIP, the most recent version is 2.04G.
These files are trojan horses and any attempt to execute them
will erase the C: drive. Please tell all your friends and
favorite BBS (Bulliten Board Services) about this hack.

Bill Teal


----- End of forwarded message -----




From: hmeekes-at-biosci.mbp.missouri.edu (Herman Meekes)
Date: Tue, 6 Jun 1995 14:05:11 -0500
Subject: Re: Software - MaComis

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subscribe microscopy {hmeekes-at-biosci.mbp.missouri.edu}






From: emlab-at-ucsco.ucsc.edu (Jon Krupp)
Date: Tue, 6 Jun 1995 16:21:06 -0700
Subject: Help Identifying Nikon Scope

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Message-Id: {199506062320.QAA02795-at-ucsco.ucsc.edu}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

I need some help identifying a Nikon compound microscope. It belongs to a
retired professor who used it for a few years and now needs to know how to
describe it for possible sale. Of course the original documentation has
been misplaced and he does not remember the details of the original order.
So, in addition to trying to get help from Nikon, I thought I would try
here too.

The instrument was purchased in 1984 for about $12,000. It looks like a
standard compound microscope on a larger than normal stand. The base is
about 18" square and it looks like the microscope body can be focused up
about 6" to 10" from the stage. It has both transmitted and reeflected
light sources provided by fiber optics.

The stage is this instruments claim to fame according to its owner. It is
large, big enough to hold an oversize thin section of rock maybe 6" x 6".
It has precision XY controls that somehow interacted with a computer
through a Nikon Digital Counter to record coordinates. The stage drives are
manual, but look like they could enclose motor drives.

That's about all he can remember, if you think you can help, please let me know.

Thanks

Jonathan Krupp
Microscopy and Imaging Lab
University of California
Santa Cruz, CA 95064
(408) 459-2477
emlab-at-ucsco.ucsc.edu






From: :      ARRELL-at-jrc.nl
Date: Wed, 7 Jun 1995 10:37:27 GMT+0200
Subject: unsubscribe

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Message-Id: {MAILQUEUE-101.950607103727.608-at-FS-IAM-1.JRC.NL}

UNSUBSCRIBE ARRELL-at-JRC.NL




From: dietmar.reiter-at-uibk.ac.at (Dietmar Reiter)
Date: Wed, 7 Jun 1995 12:58:26 +0100
Subject: water soluble embedding media

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For embedding of small invertebrates in whole-mount immunocytochestry:
which water soluble embedding media - preserving fluorescence is not
necessary - are recommended?

Thanks for any suggestions, -Dietmar-


+++ Dietmar Reiter, Dept. of Zoology and Limnology
+++ University of Innsbruck
+++ Technikerstrasse 25, A-6020 Innsbruck, Austria
+++ phone: (43)-512-507 ext. -6170, fax ext. -2930






From: :      ARRELL-at-jrc.nl
Date: Wed, 7 Jun 1995 13:21:14 GMT+0200
Subject: UNSUBSCRIBE ARRELL@JRC.NL

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UNSUBSCRIBE ARRELL-at-JRC.NL




From: Mev H van der Merwe :      HVDM-at-op1.up.ac.za
Date: Wed, 7 Jun 1995 15:51:54 GMT+2
Subject: staining problems

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Hallo

I am using the Immuno-Bed kit for Histology. When staining the
Histology section, I get a coloured back ground in the plastic. Can
someone please help me?

Thanks

E.mail: HvdM-at-opl.up.ac.za




From: Alec Madsen :      alecm-at-u.washington.edu
Date: Wed, 7 Jun 1995 08:49:23 -0700 (PDT)
Subject: unsubscribe

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UNSUBSCRIBE ALECM-at-U.WASHINGTON.EDU




From: hukee.margaret-at-mayo.EDU (Marge Hukee)
Date: Wed, 7 Jun 1995 11:07:52 +0200
Subject: listserver vs microscopy

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Message-Id: {9506071606.AA20134-at-fermat.Mayo.EDU}
X-Sender: hukem-at-fermat.mayo.edu
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Dear subscribers;

I grow weary of subscribe and unsubscribe messages. Please send these
commands to listserver-at-aaem.amc.anl.gov and NOT to
microscopy-at-aaem.amc.anl.gov

Thanks
This message is small enough to post by your machine

Marge Hukee
EM Core Facility hukee.margaret-at-mayo.edu
Mayo Foundation (507) 284-3148
----------------------------------------------------------------------------
--






From: Elinor Solit :      cambrex-at-world.std.com
Date: Wed, 7 Jun 1995 12:19:25 +0059 (EDT)
Subject: Re: Help Identifying Nikon Scope

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Jon,

I suggest your friend contact Nikon in Long Island, New York. Their
number is 516-547-8500. Ask for any of their Product Managers, they are
all capable.

Hope this helps.

Ellie Solit
The Microscope Book

On Tue, 6 Jun 1995, Jon Krupp wrote:

} I need some help identifying a Nikon compound microscope. It belongs to a
} retired professor who used it for a few years and now needs to know how to
} describe it for possible sale. Of course the original documentation has
} been misplaced and he does not remember the details of the original order.
} So, in addition to trying to get help from Nikon, I thought I would try
} here too.
}
} The instrument was purchased in 1984 for about $12,000. It looks like a
} standard compound microscope on a larger than normal stand. The base is
} about 18" square and it looks like the microscope body can be focused up
} about 6" to 10" from the stage. It has both transmitted and reeflected
} light sources provided by fiber optics.
}
} The stage is this instruments claim to fame according to its owner. It is
} large, big enough to hold an oversize thin section of rock maybe 6" x 6".
} It has precision XY controls that somehow interacted with a computer
} through a Nikon Digital Counter to record coordinates. The stage drives are
} manual, but look like they could enclose motor drives.
}
} That's about all he can remember, if you think you can help, please let me know.
}
} Thanks
}
} Jonathan Krupp
} Microscopy and Imaging Lab
} University of California
} Santa Cruz, CA 95064
} (408) 459-2477
} emlab-at-ucsco.ucsc.edu
}
}




From: Glen Macdonald :      glenmac-at-u.washington.edu
Date: Wed, 7 Jun 1995 11:50:23 -0700 (PDT)
Subject: Re: staining problems

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X-Sender: glenmac-at-homer01.u.washington.edu

What stain are you using? I often destain with 35% ethanol to wash off
the stain, followed by distilled water do eliminate spotting left behind
by the ethanol.


Glen MacDonald
Hearing Development Laboratories
University of Washington
Seattle, WA 98195-3515
(206)543-8360
glenmac-at-u.washington.edu


On Wed, 7 Jun 1995, Mev H van der Merwe wrote:

} Hallo
}
} I am using the Immuno-Bed kit for Histology. When staining the
} Histology section, I get a coloured back ground in the plastic. Can
} someone please help me?
}
} Thanks
}
} E.mail: HvdM-at-opl.up.ac.za
}




From: Fred Hayes :      fahayes-at-ucdavis.edu
Date: Wed, 7 Jun 1995 15:53:38 -0700 (PDT)
Subject: sectioning polyester filters for LM

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Does anyone have experience thick sectioning the "Transwell Clear"
filters from Corning Costar? They thin section (80nm) fine without any
problems, but appear to have no elasticity at the light level(1000nm).
This is a 12mm polyester filter with cultured RPE cells. The customer
wants a cross sectional view since he is interested in quantifying
basement membrane deposition. The filters are embedded in EMbed 812 with
plenty of resin on both sides of the filter. Block face measures 0.5 x 5.0mm
Blocks or filter discs are bisected with that edge being sectioned.

1) the problem is section wrinkling since the filter refuses to stretch
with the resin and monolayer. I've tried differing the temp, using acetone,
toulene, Etoh, and Chloroform. Is there a solvent that permeates or
softens polyester?

Fred A. Hayes 916-752-7712 work
University of California,Davis 916-752-4701 work
School of Medicine
Department ofMedical Pathology; EM Lab
MSIA E-mail:
Davis, CA 95616 fahayes-at-ucdavis.edu

1320 Dogwood Court 916-678-6280 home
Dixon, CA 95620-3227







From: m.dickson-at-unsw.edu.au (Melvyn R. Dickson)
Date: Thu, 8 Jun 1995 10:27:41
Subject: Re: SEM-x.s. microporous PTFE membrane

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To: microscopy-at-aaem.amc.anl.gov



} Hello:

} First use of the forum... Is there is a technique for preparing cross
} section profiles of microporous, multi-layered PTFE membranes
} approximately 10 to 50 micrometers thick for FE-SEM.

We do a lot of microporous membranes for FESEM examination. Mostly they crack
quite well if we chill them under liquid nitrogen and crack them with pre
cooled forceps. But I have also had to tear them and at times chill them and
chop them with a chilled razor blade hit with a hammer. We have a Balzers BAF
400 but we seldom use it for cross fractures. After fracturing we use around
2 nm of chromium to coat.

For studying penetration down into pores we just cross sectioned epoxy
embedded membranes and looked at them with TEM. Its the only way if membranes
are still flexible at Liquid N2 temperatures. Anyway I distrust procedures
that remove material as I reckon the fouling material / coating material
whatever is likely to move also.

Mel Dickson




From: Mev H van der Merwe :      HVDM-at-op1.up.ac.za
Date: Thu, 8 Jun 1995 13:13:05 GMT+2
Subject: Glass knives for Immuno-Bed sections

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I must cut Immuno-Bed sections 25mm x 20mm. I have a problem with my
steel knife. I always get scars on the sections. I sharpend my
knife at an angle of 36 and for the finishing process 5-10 minutes
at an angle of 35. I sharpend the knife for 4-5 days. I have glass
for a ralph knife, but this glass is too soft and only suitable for
wax sections. The surface of E/M glass is too small, I prefer
cutting with a glass knife. Can someone please help me?

Thanks
Hildagoenda v.d. Merwe


E-MAIL: HvdM-at-opl.ac.za




From: Mev H van der Merwe :      HVDM-at-op1.up.ac.za
Date: Thu, 8 Jun 1995 14:45:24 GMT+2
Subject: glass knives

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I must cut Immuno-Bed sections 25mm x 20mm. I have a problem with my
steel knife. I always get scars on the sections. I sharpend my
knife at an angle of 36 and for the finishing process 5-10 minutes at
an angle of 35. I sharpend the knife for 4-5 days.

I have glass for a ralph knife but this glass is too soft and only
suitable for wax sections. The surface of E/M glass is too small.

I prefer cutting with a glass knife. Can someone help please.

Thanks

Hildagoenda v.d. Merwe
University of Pretoria
South Africa


E-mail: HVDM-at-opl.up.ac.za




From: MacisNo1-at-aol.com
Date: Thu, 8 Jun 1995 09:30:50 -0400
Subject: Re: Help with Nikon Scope

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Jonathan Krupp,

It sounds to me like either UM or TM20. These were large pillar type
"Toolmakers" microscopes or also known as Measuring scopes due to the long
throat (to accommodate large specimens) and accurate linear stages. Some were
manual as yours with micrometers and others use scales with LED readouts. In
any event. let me know what color the stand is and that will help me further
identify it.

Sincerely,

Larry Kordon
Nikon, Inc.
MacisNo1-at-aol.com




From: farmer-at-emlab.zoo.uga.edu
Date: Thu, 8 Jun 1995 10:11:50 +0000
Subject: Glycogen in TEM

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Message-Id: {9506081411.AA14319-at-emlab.zoo.uga.edu}
Comments: Authenticated sender is {farmer-at-emlab.zoo.uga.edu}

Does anyone have a good method for positively staining glycogen in
the TEM? I am aware of glycogen's staining characteristics when it
reacts with lead salts and the importance of OsO4 pH in affecting the
appearance of glycogen. Unfortunately these and other techniques
(e.g. agglutination with Con A) are not specific enough for this
project. We need to demonstrate glycogen and nothing but glycogen.

Are there any commercially available enzyme-colloidal gold complexes
that might be of use? Any references or advice would be
appreciated.

Mark Farmer

Mark A. Farmer
Director, Ctr. Ultrastructural Research
University of Georgia, Athens, GA 30602
(706)542-4080 Voice (706)542-4271 FAX
farmer-at-emlab.zoo.uga.edu





From: Paul Webster :      Paul.Webster-at-quickmail.cis.yale.edu
Date: 8 Jun 1995 11:00:58 -0400
Subject: Glycogen in TEM

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Message-Id: {n1409513192.93718-at-QuickMail.Yale.edu}

Use antibodies to glycogen.





From: hukee.margaret-at-mayo.EDU (Marge Hukee)
Date: Thu, 8 Jun 1995 10:52:38 +0200
Subject: sectioning immuno-bed (25mmX20mm)

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We used to section JB-4 with the fresh broken edge of a microscope slide
that was inserted into a razor blade adapter. The older ultratomes had
these adapters, but with the new machines you may have to have a machinist
make one for you. A machine that uses metal knives usually includes a
holder for the disposable knives that may be also adaptable.
For what it's worth
Marge

Marge Hukee
EM Core Facility hukee.margaret-at-mayo.edu
Mayo Foundation (507) 284-3148
----------------------------------------------------------------------------
--






From: masur-at-msvax.mssm.edu
Date: Thu, 08 Jun 1995 13:56:41 -0500
Subject: double stain with two mouse monoclonals

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Has anyone had success with immunofluorescent co-localization of two
antigens, on one coverslip of cultured cells using using two different
mouse monoclonal antibodies. (we have had results in which we could not be
certain that we had successfully blocked after the first antibody)

protocol please

Sandra K. Masur Box 1183
fax: 212-289-5945 Mount Sinai School of Medicine
phone: 212-241-6544 or 0089 NY NY 10029-6574
e-mail:masur-at-msvax.mssm.edu









From: Paul Webster :      Paul.Webster-at-quickmail.cis.yale.edu
Date: 8 Jun 1995 15:00:07 -0400
Subject: Double stain with two mouse

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Message-Id: {n1409498732.63153-at-QuickMail.Yale.edu}

Sandra K. Masur writes:
Has anyone had success with immunofluorescent co-localization of two
antigens, on one coverslip of cultured cells using using two different
mouse monoclonal antibodies. (we have had results in which we could not be
certain that we had successfully blocked after the first antibody).

It would be interesting to know how you blocked after the first antibody. There
is no blocking protocol that I know of which will stop antibodies binding to
antigens.

As far as I know there is no published protocol for double labeling with two
monoclonal antibodies on the same preparation. The normal way is to directly
label the antibodies with fluorescent tags and not use secondary antibodies. It
should also be easy to label one of the monoclonals with a small antigenic
molecule such as biotin and detect it with anti-biotin antibodies (or
FITC-streptavidin).

If you were using sections I would suggest cutting serial sections, labeling one
set with the first antibody and the other with the second.








From: Michael Rock :      merock-at-u.washington.edu
Date: Thu, 8 Jun 1995 09:32:09 -0700 (PDT)
Subject: Re: sectioning polyester filters for LM

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Fred
you might try using a vacuum dessicator to remove the wrinkles, it seem
to work well for 1 micron sections on glass, it may work for TEM as well.
-Mike

On Wed, 7 Jun 1995, Fred Hayes wrote:

}
} Does anyone have experience thick sectioning the "Transwell Clear"
} filters from Corning Costar? They thin section (80nm) fine without any
} problems, but appear to have no elasticity at the light level(1000nm).
} This is a 12mm polyester filter with cultured RPE cells. The customer
} wants a cross sectional view since he is interested in quantifying
} basement membrane deposition. The filters are embedded in EMbed 812 with
} plenty of resin on both sides of the filter. Block face measures 0.5 x 5.0mm
} Blocks or filter discs are bisected with that edge being sectioned.
}
} 1) the problem is section wrinkling since the filter refuses to stretch
} with the resin and monolayer. I've tried differing the temp, using acetone,
} toulene, Etoh, and Chloroform. Is there a solvent that permeates or
} softens polyester?
}
} Fred A. Hayes 916-752-7712 work
} University of California,Davis 916-752-4701 work
} School of Medicine
} Department ofMedical Pathology; EM Lab
} MSIA E-mail:
} Davis, CA 95616 fahayes-at-ucdavis.edu
}
} 1320 Dogwood Court 916-678-6280 home
} Dixon, CA 95620-3227
}
}
}
}




From: hukee.margaret-at-mayo.EDU (Marge Hukee)
Date: Thu, 8 Jun 1995 16:36:16 +0200
Subject: DOUBLE STAIN

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Fixatives generally will alter the ability of an antibody to react to a
second antibody. This can be easily tested by trying to label your first
antigen after pretreatment with fixative. A paper is available in the
literature describing a blockage of first antibody with vapor from
formaldehyde fixative generated at 80 degrees C and subsequent labeling
with a second antibody of the same specie. This paper is by Wang and
Larsson and can be found in Histochemistry bol.83:page 47, 1985. I have
used this method successfully at the electron microscopy level. If I can
be of any further help, please feel free to contact me.
Marge

Marge Hukee
EM Core Facility hukee.margaret-at-mayo.edu
Mayo Foundation (507) 284-3148
----------------------------------------------------------------------------
--






From: David Garrett :      DGARRETT-at-gab.unt.edu
Date: Thu, 8 Jun 1995 16:46:15 CST6CDT
Subject: Question: Re: sectioning polyester filters for LM

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} Date sent: Thu, 8 Jun 1995 09:32:09 -0700 (PDT)
} From: Michael Rock {merock-at-u.washington.edu}
} To: Fred Hayes {fahayes-at-ucdavis.edu}
} Copies to: microscopy-at-aaem.amc.anl.gov
} Subject: Re: sectioning polyester filters for LM

} Fred
} you might try using a vacuum dessicator to remove the wrinkles, it seem
} to work well for 1 micron sections on glass, it may work for TEM as well.
} -Mike
}
Please explain.
Thanks
***************************************
David Garrett "DGARRETT-at-GAB.UNT.EDU"
University of North Texas
Dept. Biological Sciences
(817)565-3964 Fax (817)565-4136
***************************************




From: hukee.margaret-at-mayo.EDU (Marge Hukee)
Date: Thu, 8 Jun 1995 17:19:03 +0200
Subject: glycogen staining

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Message-Id: {9506082217.AA10481-at-fermat.Mayo.EDU}
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Dear Mark,
Glycogen can be stained by adding potassium ferricyanide to primary and
secondary fixatives. If you wish to differentiate glycogen B-particles
from ribosomes, do not use lead stain on grids. References are available
in the literature. Two that I know of are: Duvall, Hukee in Ann. Otol.
Rhinol. Laryngol. vol 85:234, 1976 this technique was modified from De
Bruijn in Histochem Journal vol. 8:121,1976 or an earlier paper by this
same author. In more recent times De Bruijn has looked at various
fixatives with x-ray microanalysis in Histochemical Journal vol.13:125,
1981.

Marge Hukee
EM Core Facility hukee.margaret-at-mayo.edu
Mayo Foundation (507) 284-3148
----------------------------------------------------------------------------
--






From: images1-at-biosci.mbp.missouri.edu (Michael Stanley)
Date: Thu, 8 Jun 1995 05:26:22 -0500
Subject: Double stain with two mouse

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Message-Id: {v01510101abfc7e3931ae-at-[128.206.15.185]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

} Date: 8 Jun 1995 15:00:07 -0400
} From: "Paul Webster" {Paul.Webster-at-quickmail.cis.yale.edu}
} Subject: Double stain with two mouse
} To: "Microscopy Bulletin Board" {microscopy-at-AAEM.AMC.ANL.GOV}
}
} Sandra K. Masur writes:
} Has anyone had success with immunofluorescent co-localization of two
} antigens, on one coverslip of cultured cells using using two different
} mouse monoclonal antibodies. (we have had results in which we could not be
} certain that we had successfully blocked after the first antibody).
}
} It would be interesting to know how you blocked after the first antibody.
} There
} is no blocking protocol that I know of which will stop antibodies binding to
} antigens.
}
} As far as I know there is no published protocol for double labeling with two
} monoclonal antibodies on the same preparation. The normal way is to directly
} label the antibodies with fluorescent tags and not use secondary antibodies. It
} should also be easy to label one of the monoclonals with a small antigenic
} molecule such as biotin and detect it with anti-biotin antibodies (or
} FITC-streptavidin).
}
} If you were using sections I would suggest cutting serial sections,
} labeling one
} set with the first antibody and the other with the second.
}
}
There are a couple of papers available: Stefan Wurden and Uwe Homberg, The
Journal of Histochem and Cytochem, vol 41, pg 627, 1993. And S.A.L. Carl,
Gillete-Ferguson, and D.G. Fertuson, J of Histochem and Cytochem, vol 41,
pg 1273, 1993.

We have a user that finally managed to get a protocol to work but it took
months...

C. Michael Stanley, Ph.D.
Coordinator, Associate Director
Molecular Cytology Core Facility
Molecular Biology Program
2 Tucker Hall
University of Missouri-Columbia
Columbia, MO. 65211
(314) 882-4895
fax= 314-882-0123






From: tphillips-at-biosci.mbp.missouri.edu (Tom Phillips)
Date: Thu, 8 Jun 1995 16:36:08 -0500
Subject: Re: Double stain with two mouse

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There are, in fact, several articles in which a single section has been
stained with 2 monoclonals from the same species. e.g., Lewis-Carl et al.,
J. Histochem. Cytochem 1993 41:1273-1278
Boorsma Histochemistry 1984; 80:103
an der Loos e tal., 1987 J. Histochem Cytochem 1987 35:1139
Carl et al, J. Histochem Cytochem 1993 41:1273
and a bunch of others.
See these papers for the controls that have been used.
An alternative approach is to stain a section with a monoclonal,
photograph, strip the antigen and re-stain with a second antibody. We have
an abstract at the coming EMSA meeting on this approach (Stanley and
Phillips) but others have also tried this approach: one example is Lan et
al., J. Histochem. Cytochem 1995, 43:97
}
} } Sandra K. Masur writes:
} } Has anyone had success with immunofluorescent co-localization of two
} } antigens, on one coverslip of cultured cells using using two different
} } mouse monoclonal antibodies. (we have had results in which we could not be
} } certain that we had successfully blocked after the first antibody).
} }
} } It would be interesting to know how you blocked after the first antibody.
} } There
} } is no blocking protocol that I know of which will stop antibodies binding to
} } antigens.
} }
} } As far as I know there is no published protocol for double labeling with two
} } monoclonal antibodies on the same preparation. The normal way is to directly
} } label the antibodies with fluorescent tags and not use secondary
} } antibodies. It
} } should also be easy to label one of the monoclonals with a small antigenic
} } molecule such as biotin and detect it with anti-biotin antibodies (or
} } FITC-streptavidin).
} }
} } If you were using sections I would suggest cutting serial sections,
} } labeling one
} } set with the first antibody and the other with the second.
} }
}

Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility
3 Tucker Hall
University of Missouri
Columbia, MO 65211
(314)-882-4712 (voice)
(314)-882-0123 (fax)






From: lmiller-at-ux1.cso.uiuc.edu (Lou Ann Miller)
Date: Thu, 8 Jun 1995 20:59:53 -0700
Subject: Re: glycogen staining

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Content-Type: text/plain; charset="us-ascii"

Hi Mark,

Just a little note to this,

Be sure NOT to enbloc stain with Uranyl Acetate after the KCN if using UA
is your standard procedure. UA will pull the glycogen back out of your
sample.

Lou Ann



} Dear Mark,
} Glycogen can be stained by adding potassium ferricyanide to primary and
} secondary fixatives. If you wish to differentiate glycogen B-particles
} from ribosomes, do not use lead stain on grids. References are available
} in the literature. Two that I know of are: Duvall, Hukee in Ann. Otol.
} Rhinol. Laryngol. vol 85:234, 1976 this technique was modified from De
} Bruijn in Histochem Journal vol. 8:121,1976 or an earlier paper by this
} same author. In more recent times De Bruijn has looked at various
} fixatives with x-ray microanalysis in Histochemical Journal vol.13:125,
} 1981.
}
} Marge Hukee
} EM Core Facility hukee.margaret-at-mayo.edu
} Mayo Foundation (507) 284-3148
} ----------------------------------------------------------------------------
} --

***************************
Lou Ann Miller MT(ASCP) CT(MSA)
Microscopic Imaging Lab
College of Vet. Medicine
University of Illinois
2001 S Lincoln Ave
Urbana,Illinois 61801
217-244-1566
lmiller-at-ux1.cso.uiuc.edu






From: A. HONARBAKHSH-RAOUF :      CER5AH-at-ECU-01.NOVELL.LEEDS.AC.UK
Date: Fri, 9 Jun 1995 08:10:27 GMT
Subject: cast iron thin foil

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Hello
Does anyone know any suitable solution for preparation of thin
foil using jet polishing of ductile cast iron(presence of two
different phases ie free graphite and matrix)? Is there any other
method except ion beam?
Thanks
Abbas




From: Neuberger, Damian
Date: Thursday, June 08, 1995 4:18PM
Subject: MRS-3 Mag Standard

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Has anybody used the Geller MicroAnalytical MRS-3XYZ NIST
traceable magnification standard. It is reported to be traceable in XY
and Z axes and can be used for SEM and optical microscopes
(including confocal in reflected mode?). Comments on experience
with its use and performance would be much appreciated.

Damian Neuberger
neuberd-at-roundlake.baxter.com




From: Witold Zielinski :      WIZIEL-at-inmat.pw.edu.pl
Date: Fri, 9 Jun 1995 14:56:49 CET
Subject: Re: cast iron thin foil

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*From: "A. HONARBAKHSH-RAOUF" {CER5AH-at-ECU-01.NOVELL.LEEDS.AC.UK}
*To: microscopy-at-aaem.amc.anl.gov
*Date sent: Fri, 9 Jun 1995 08:10:27 GMT
*Subject: cast iron thin foil
*Priority: normal

*Hello
*Does anyone know any suitable solution for preparation of thin
*foil using jet polishing of ductile cast iron(presence of two
*different phases ie free graphite and matrix)? Is there any other
*method except ion beam?
*Thanks
*Abbas

If the particles are small, like mikron size, try mixture of
95% acetic accid and 5% perchloric accid. Room temp! and voltage
about 80 - 90 Volts.
Good luck
Witold Zielinski
Warsaw University of Tech.
Warszawa




From: gbza40-at-udcf.gla.ac.uk
Date: Fri, 9 Jun 1995 14:14:44 +0100
Subject: MORE ON GLYCOGEN STAINS

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Message-Id: {9652.199506091314-at-lenzie.cent.gla.ac.uk}

Dear Mark Farmer

No one's mentioned the Thiery on-section method for carbohydrate staining
which shows up glycogen extremely well through silver deposition on
unstained epoxy sections. It may be an old method but it is very precise
and allows resolution of glycogen substructure if the reaction is time
varied to give optimal contrast. There's usually little background at
moderate magnifications and it works on routine glut/OsO4 blocks thus giving
good ultrastructure for this kind of electron cytochemistry.

Original paper is Thiery (1967), but the method is described in Hayat's book
Positive Staining for EM (1975) p111 and in many other texts also.

Whether this is sufficient for your needs is for you to decide !

Good luck

Laurence Tetley
EM Centre Biological Lab
Institute of Biomed and Life Sciences
University of Glasgow
Scotland, UK





From: Goldmarker-at-aol.com
Date: Fri, 9 Jun 1995 11:00:19 -0400
Subject: In-Situ hybridization w/ gold?

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Does anyone have a good reference(s) and/or protocol for the use of
anti-digoxigenin gold or anti-biotin gold at either the EM or LM level?

I am trying to gather as much material as possible to help others with
in-situ hybridization with gold techniques.

In addition, if anyone would like to have a "direct" and "indirect" SAD-gold
(1nm) procedure, I'll be happy to send it.

Regards, Donald P. Cox, Ph.D.
Goldmark Biologicals
437 Lock St
Phillipsburg, NJ 08865
(908) 859-2631 - - - (908) 859-2875-FAX
E-mail: goldmarker-at-aol.com




From: David Henriks :      73531.1344-at-compuserve.com
Date: 09 Jun 95 11:29:59 EDT
Subject: cast iron thin foil

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You are certainly trying to thin some difficult material!

A good starting point would be:

65ml hydrochloric acid
435 ml methanol
20 ml butyl cellosolve

Temperature: -20 degrees C
Voltage: 110V
Current: 120 mA

These conditions are as used on a South Bay Technoilogy Model 550 Single
Vertical Jet Electropolisher. If you are using a twin-jet system, you will need
to adjust some of the parameters, but the basic solution is a good starting
point.

You may want to refere to the following paper:

"Technique for Jet Thinning Aged Iron-Chromium Alloys for TEM"
B.J. Kestel
Ultramicroscopy 25 (1988) 89-90

If you prefer, I could send you a copy of the paper at no charge. Of course, I
would also be pleased to send you information on our jet polisher and/or any of
our other sample preparation products.

Best regards-





From: David Henriks, 73531,1344
Date: 09 Jun 95 11:34:14 EDT
Subject: Copy of: cast iron thin foil

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---------- Forwarded Message ----------


RE: Copy of: cast iron thin foil

You are certainly trying to thin some difficult material!

A good starting point would be:

65ml hydrochloric acid
435 ml methanol
20 ml butyl cellosolve

Temperature: -20 degrees C
Voltage: 110V
Current: 120 mA

These conditions are as used on a South Bay Technoilogy Model 550 Single
Vertical Jet Electropolisher. If you are using a twin-jet system, you will need
to adjust some of the parameters, but the basic solution is a good starting
point.

You may want to refere to the following paper:

"Technique for Jet Thinning Aged Iron-Chromium Alloys for TEM"
B.J. Kestel
Ultramicroscopy 25 (1988) 89-90

If you prefer, I could send you a copy of the paper at no charge. Of course, I
would also be pleased to send you information on our jet polisher and/or any of
our other sample preparation products.

Best regards-

David Henriks TEL: 800-728-2233 (toll-free in USA)
South Bay Technology, Inc. 714-492-2600
1120 Via Callejon FAX: 714-492-1499
San Clemente, CA 92673 USA e-mail: 73531.1344-at-compuserve.com





From: bozzola-at-siu.edu (John. J. Bozzola)
Date: Fri, 9 Jun 1995 11:43:44 -0600
Subject: allEM: Geller Mag Standard MRS-3XYZ NIST

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Anyone have ordering information & pricing on the Geller MicroAnalytical
MRS-3XYZ NIST magnification standard? Thank you.


John J. Bozzola
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
Phone: 618-453-3730
Fax: -2665
Email: bozzola-at-siu.edu OR bozzola-at-qm.c-cem.siu.edu






From: bozzola-at-siu.edu (John. J. Bozzola)
Date: Fri, 9 Jun 1995 14:41:38 -0600
Subject: TEM

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Regarding hi res CCD cameras (1024x1024) for the TEM (Hitachi H7100), does
anyone know of a reliable vendor who might be able to supply & install a
working camera within 4-6 weeks? Thank you kindly.

John J. Bozzola
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
Phone: 618-453-3730
Fax: -2665
Email: bozzola-at-siu.edu OR bozzola-at-qm.c-cem.siu.edu






From: Eugene Bluhm :      bluhm-at-umr.edu
Date: Fri, 9 Jun 1995 15:07:37 -0600 (CDT)
Subject: subscribe bluhm@umr.edu

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subscribe bluhm-at-umr.edu




From: bozzola
Date: Friday, June 09, 1995 11:43AM
Subject: allEM: Geller Mag Standard MRS-3XYZ NIST

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Anyone have ordering information & pricing on the Geller MicroAnalytical
MRS-3XYZ NIST magnification standard? Thank you.


John J. Bozzola
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
Phone: 618-453-3730
Fax: -2665
Email: bozzola-at-siu.edu OR bozzola-at-qm.c-cem.siu.edu






From: tvoiles-at-unlinfo.unl.edu (Todd Voiles)
Date: Fri, 09 Jun 1995 14:24:21 -0400
Subject: Getting Users to Use Equipment Properly

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Message-Id: {9506091923.AA28231-at-unlinfo.unl.edu}
X-Sender: tvoiles-at-129.93.1.11 (Unverified)
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He at U of Nebraska we have been running into the re-occuring problem
of users of the scopes and lab being overly messy and careless with
the equipment. We've tried posting signs about it, writing standard and
emergency operating proccedures to no avail. I'm sure other people
have had similar problems in their labs........anybody want to share
some solutions?


Todd Voiles
Facility Manager
Central Facility for Electron Microscopy
Center for Materials Research and Analysis

University of Nebraska at Lincoln

tvoiles-at-unlinfo.unl.edu





From: baskin-at-biosci.mbp.missouri.edu (Tobias Baskin)
Date: Fri, 9 Jun 1995 16:14:17 -0500
Subject: *M/fixatives/pfa

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Greetings,
Anyone have a protocol for dissolving powder paraformaldehyde in
acetone???
Thanks,
Tobias Baskin

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
___ ____ ^ ____ _____ Tobias I. Baskin
/ \ / / \ / \ / University of Missouri
/ | / / \ / / Biological Sciences
/___ / /__ /_____\ / /__ 109 Tucker Hall
/ / / \ ( / Columbia, MO 65211 USA
/ / / \ \ / voice: 314-882-0173
/ /____ / \ \____/ /_____ fax: 314-882-0123






From: Joseph P. Neilly 708-938-5024 :      NEILLY.JOSEPH-at-igate.pprd.abbott.com
Date: Fri, 09 Jun 1995 08:14:00 -0600 (CST)
Subject: Re:MRS-3 Mag Standard

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Mr-Received: by mta RANDB; Relayed; Fri, 09 Jun 1995 10:36:22 -0600
Mr-Received: by mta MCM$RAND; Relayed; Fri, 09 Jun 1995 10:36:24 -0600
Mr-Received: by mta RANDC; Relayed; Fri, 09 Jun 1995 10:36:37 -0600
Alternate-Recipient: prohibited
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Content-Return: prohibited

Damian,

We purchased the MRS-2 a couple of years ago and use it routinely for
both SEM and optical microscopy (both reflected and transmitted). I
believe Geller has added some video patterns on the MRS-3 but the basic
pattern has not changed. The best feature of this standard is the
ability to do X and Y checks with a single image. We do have a little
difficulty getting good contrast at low kV (2 kV) in the SEM in the
secondary mode. The only other problem we have with this standard is the
fact that it is so useful for both SEM and optical that it never stays
in the same lab very long.

Joe Neilly
Cellular and Microscopic Research
Abbott Laboratories
Abbott Park, IL
E-mail: neilly.joseph-at-igate.abbott.com
Phone: (708)-938-5024






From: Gib Ahlstrand :      giba-at-puccini.crl.umn.edu
Date: Fri, 9 Jun 1995 18:39:11 -0500
Subject: Re: Getting Users to Use Equipment Properly

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In message {9506091923.AA28231-at-unlinfo.unl.edu} Todd Voiles writes:
} He at U of Nebraska we have been running into the re-occuring problem
} of users of the scopes and lab being overly messy and careless with
} the equipment. We've tried posting signs about it, writing standard and
} emergency operating proccedures to no avail. I'm sure other people
} have had similar problems in their labs........anybody want to share
} some solutions?



Its hopeless, Todd. I recognize your malady right off the bat. You've got dem
ol' EM Lab manager blues. You must learn to float above it all with equanimity,
for your own peace of mind, and keep your service contracts current. As one
hoary old sage of EM put it: "Be in the lab, but not of it". There's nothing
like managing a service lab to sharpen up your spiritual practice.

Stay cool!

Gib

--

Gib Ahlstrand, MMS Newsletter Editor
Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
495 Borlaug Hall, St. Paul, MN 55108 (612)625-8249
612-625-9728 FAX, giba-at-puccini.crl.umn.edu

"MICROSCOPY & MICROANALYSIS 96" in Minneapolis, Minnesota, Aug.11-15, 1996





From: Gib Ahlstrand :      giba-at-puccini.crl.umn.edu
Date: Fri, 9 Jun 1995 19:01:58 -0500
Subject: Re: Getting Users to Use Equipment Properly

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In message {9506091923.AA28231-at-unlinfo.unl.edu} Todd Voiles writes:
} He at U of Nebraska we have been running into the re-occuring problem
} of users of the scopes and lab being overly messy and careless with
} the equipment. We've tried posting signs about it, writing standard and
} emergency operating proccedures to no avail. I'm sure other people
} have had similar problems in their labs........anybody want to share
} some solutions?

But seriously, Todd (tho the thoughts I sent earlier ARE part of my lab attitude
part of the time) you only mention posting signs and writing operating
procedures for your lab users. Thats fine and necessary, but it really takes
face to face discussion with users about whats expected, setting the tone, and
the written stuff is just to remind them of that when you are not around. Get
your lab director or department head or some other heavyweight authority figure
to back you up, make it clear to users that they may be asked to leave if they
cannot follow a few reasonable operating procedures. In my experience, most will
do that if approached in the right way, but there are always a few...........

Sometimes I get better results with younger people, students. Just screw off the
top of their head, pour in your information, screw their cover back on, give
them a pat on the butt and they are off and running. Sometimes its the older
experienced, rusty crusty heads whose lab habits are set that are the difficult
ones to communicate with. Take a workshop on management to get some ideas.

Good luck. Gib



--

Gib Ahlstrand, MMS Newsletter Editor
Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
495 Borlaug Hall, St. Paul, MN 55108 (612)625-8249
612-625-9728 FAX, giba-at-puccini.crl.umn.edu

"MICROSCOPY & MICROANALYSIS 96" in Minneapolis, Minnesota, Aug.11-15, 1996





From: AWBlackwoo-at-aol.com
Date: Sat, 10 Jun 1995 12:11:32 -0400
Subject: Re: Getting Users, etc.

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This is every bit as much a problem for the commercial laboratory as for the
academic scene. At Structure Probe, we have a multi-user, multi-operator
laboratory doing SEM and TEM for both biological and materials science
applications, including asbestos. In addition to all of the problems of
clutter and mess in any laboratory, we have the added risk of
cross-contamination. As the laboratory director, I can tell you that the
suggestions to get some "heavyweight" involved really don't help; giving
orders doesn't motivate the employees of the 1990's any better than it
inspires students or "crusty heads".

We are, however, having some success in appling the principles of Total
Quality Management (TQM) to this problem. TQM has been very successful for
us in reducing problems such as shipping the wrong product or the wrong
quantity of the right product to the customers of our SPI Supplies Division.
The same principles can work in any environment, including the laboratory.

Basically, we have let the people who use the laboratory work out their own
solution, with some guidance; this is one of the essentials of the TQM
approach. Their "solution" is to apply peer pressure to clean up, but on a
rotating basis, every laboratory employee is accountable to management for a
week for the cleanliness of the facilities. This is not the way I would have
directed that things be done, but because it came from the users (and
offenders) themselves, it works well.

I would be happy to dialog with anybody on the subject of quality management
in the laboratory environment.

Andy Blackwood

Andrew W. Blackwood, Ph.D.
Structure Probe, Inc.
63 Unquowa Road
Fairfield, CT 06430-5015
Phone: 1-203-254-0000
FAX: 1-203-254-2262
e-mail: AWBlackwoo-at-aol.com





From: allan.mitchell-at-stonebow.otago.ac.nz (Allan Mitchell)
Date: Mon, 12 Jun 1995 12:37:02 +1200
Subject: SEM verses TEM usage

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Ken and Terry,
Greatings from the deep south. We to were amalgamated about 5 years ago.
The technical staff of our Unit are funded from 3 Medical School
departments, 0.5 person from Micriobiology, 2.5 persons from Anatomy, 2
persons from Pathology.

Although housed in the Medical School we work for all the science
departments except the Dental School, ie Zoology, Botany, Physics,
Biochemistry, Marine Science, Geology etc.

3 have 3 TEM's and 1 SEM. It runs 6 to 10 per day, 5 to 6 days per week.
Most of the work is Zoology, Marine Science and Geology, very little
Medical School SEM users. There still seems to be a lot of fish and creepy
crawlies that need to be scanned but humans have been 'done to death'.

From our point of veiw ESEM or Cold stage SEM are the way to go. At the
conference here in September (I presume you know about our conference) will
be Brendon Griffin from Perth, Guru on environmental scanners and very
approachable (he has to be, we are paying for him to be here). Oxford
instruments are bringing an expert on Co;d stages I believe so come to the
conference to get the info.

If I can help with anything else just call

Regards

Allan



---------------------------------------------------------
Allan Mitchell
Senior Technical Officer
South Campus Electron Microscope Unit
C/- Department of Anatomy and Structural Biology
Otago Medical School

P.O. Box 913
Dunedin
New Zealand

Tel; National 03 479 7301 International 64 3 479 7301
Fax; National 03 479 7254 International 64 3 479 7254

"The Southernmost E.M. Unit in the World"






From: DVCCO-at-aol.com
Date: Mon, 12 Jun 1995 02:51:09 -0400
Subject: URL Web Site Video Camera Update

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LM/EM URL Web Site Video Camera Guide Location Update!

***** After review of the web site feel free to address any questions to
((((((((((( DVC (DIRECTLY) via web site e-mail or dvcco-at-aol.com )))))))))))
***** PLEASE DO NOT TIE UP THE MICROSCOPY NET. Thank You

DVC Company wishes to introduce one of our many web sites to users of this
net, who wish to access 315876 bytes of detailed text, gifs, and performance
charts of the DVC video camera line.
------------------------------------------------------------------------
Our WWW URL Web Site is: http://www.edt.com/dvc/dvc.html
------------------------------------------------------------------------
Web files cover:
* DVC digital and analog cameras for cost effective sub-pixel accuracy
* PCI bus, Mac Nu-bus, Sun S-bus compatible board data
* Silicon Graphics (digital output) DVC cameras for Indy and Indigo 2!
* What to look for when choosing a CCD camera, (questions and answers) etc.
* Our goal is to present first hand information from the source. If you are
offended by information from whatever source, please hit your delete key now.

The complete HTML files detail:
DVC Monochrome CCD Digital/Analog Video Cameras operating at
real time video rates at 8 or 10 bits } 62dB signal to noise with no
cooling needed for extremely high sensitivity with many options.
Designed for quantitative microscopy and image analysis.
Models include:
DVC-10 RS-422 digital 10 bit with simultaneous RS-170 analog video
DVC-8 RS-422 digital 8 bit with simultaneous RS-170 analog video
DVC-0A RS-170 analog video/ ( upgradable to DVC-10/ DVC-8 models.)
This lets the researcher, cost effectively grow with the application.

* Compatible frame grabbers/ digitizers on the market with DVC offering
( the complete board, custom cable and our camera line, plug and play! )
for ( PCI bus, Mac Nu-bus, Sun S-bus, and the SGI digital out for the
Silicon Graphics Indy and Indigo 2 workstations! )

* Tunable Liquid Crystal Filters for:
* RGB sequential non real time 50ms switching from Red to Green to Blue
with no pixel alignment problems at a 50nm band width that can be
shifted by user for attachment to DVC or other monochrome cameras
or:
* Monochrome wavelength coverage from (400 - 1100nm), anywhere the user
wants to be within 50ms with 32 memories or sequential scan at any
user defined transition down to .1 nm step via RS-232 or TTL with any
user defined bandwidth from 5 to 50nm.

Request: When requesting info from DVC please offer us your:
* Complete address
* E-mail, phone, and fax
* Application, looking at what, on a microscope, low light req.
* Any special modifications, and time frame of project or need.
*** Down load as much information from our web site as possible.
DVC Company is proud to present information on a product manufactured in the
U.S.A. We send this web site update to you with the best of intentions and
trust it can be used as a guide to making the right decision when spending
precious funding on equipment. DVC realizes as our customers do, that your
camera is critical to your systems performance and not just incidental to it.





From: STEPHAN HELFER :      S.Helfer-at-rbge.org.uk
Date: Mon, 12 Jun 1995 09:58:43 BST
Subject: Re: Getting Users to Use Equipment Properly

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To: Todd Voiles {tvoiles-at-unlinfo.unl.edu}

This may be an obvious thing to do for most of you, but as it has not been
mentioned before I risk stating the obvious: accountability is the key to a
multitude of multi-user problems. Keeping an accurate log book of users and
insisting in consistent log book entry, helps pinpointing problems (genuine
ignorance about procedures or blatant negligence). Must obviously be followed
up by personal contact with the miscreants. Initially it helps to give them the
benefit of the doubt; later on disciplinary measures may have to follow (e.g.
banning from the equipment or lab).

my tupence worth

Yours sincerely


Dr Stephan Helfer, SSO
Mycologist

Royal Botanic Garden Edinburgh, Inverleith Row, EDINBURGH EH3 5LR,
Scotland UK

email s.helfer-at-rbge.org.uk
phone: +44 (0)131 552 7171 ext 280
or +44 (0)131 459 0446-280 (direct digital VoiceMail line)
fax: +44 (0)131 552 0382




From: Biquan Lin :      blin-at-mcs.anl.gov
Date: Mon, 12 Jun 1995 08:13:41 -36803936 (CDT)
Subject: unsubscribe

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UNSUBSCRIBE BLIN-at-MCS.ANL.GOV





From: Jay Jerome :      jjerome-at-isnet.is.wfu.edu
Date: Mon, 12 Jun 1995 09:20:12 -0400 (EDT)
Subject: Re: Getting Users to Use Equipment Properly

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We have a strict sign-up for microscopes, so we can identify who has used
a scope. The first time someone fails to take care of the equipment they
get a gently reminder from my staff, the second time they get a less
gentle reminder from me. Chronic problem causers are denied access to the
equipment. This final solution is not used often, but it has occurred.

That is how we have solved the problem.

Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-isnet.is.wfu.edu *
**************************************************************

On Fri, 9 Jun 1995, Todd Voiles wrote:

} He at U of Nebraska we have been running into the re-occuring problem
} of users of the scopes and lab being overly messy and careless with
} the equipment. We've tried posting signs about it, writing standard and
} emergency operating proccedures to no avail. I'm sure other people
} have had similar problems in their labs........anybody want to share
} some solutions?
}
}
} Todd Voiles
} Facility Manager
} Central Facility for Electron Microscopy
} Center for Materials Research and Analysis
}
} University of Nebraska at Lincoln
}
} tvoiles-at-unlinfo.unl.edu
}
}




From: Michael Rock :      merock-at-u.washington.edu
Date: Mon, 12 Jun 1995 09:01:13 -0700 (PDT)
Subject: Re: Getting Users to Use Equipment Properly

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Todd-
try scheduling weekly clean-up sessions, include all users of the
facility, if they never seem to attend...restrict their access.
-Mike
On Fri, 9 Jun 1995, Todd Voiles wrote:

} He at U of Nebraska we have been running into the re-occuring problem
} of users of the scopes and lab being overly messy and careless with
} the equipment. We've tried posting signs about it, writing standard and
} emergency operating proccedures to no avail. I'm sure other people
} have had similar problems in their labs........anybody want to share
} some solutions?
}
}
} Todd Voiles
} Facility Manager
} Central Facility for Electron Microscopy
} Center for Materials Research and Analysis
}
} University of Nebraska at Lincoln
}
} tvoiles-at-unlinfo.unl.edu
}
}




From: Michael Boucher :      BOUCHER-at-tcrca.usbm.gov
Date: Mon, 12 Jun 1995 14:14:14 CST
Subject: Help:Frosted Petrographic Slides

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For all you petrographers; Help!

Our lab has for years been using frosted slides for polished thin sections.
Apparently only one manufacturer is left, Erie Manufacturing (Erie Scientific,
etc) and they are not satisfactory for our needs. All suppliers keep
sending these same slides. Ward's Scientific says that Erie is the
last remaining source for frosted petrographic slides. Making our own
will be very labor and time consuming. Any sources other than Erie?

The details are that the finish is very finely frosted, and there is
little "tooth" for the epoxy, so some of the phases easily pluck out
as we reach the end of the polishing stages. This began when we ran
out of our old supply from a now defunct company and began using the
new slides. Thanks for any help, advice, etc.
Please E-mail me direct unless you believe it would benefit others on
the server. Thanks
Mike
*****************************************************************
Michael L. Boucher Sr. Boucher-at-TCRCA.USBM.GOV
Geology-Mineralogy/Chemistry Labs Ph 612-725-4614
Twin Cities Research Center Fax 612-725-4527
U.S. Bureau of Mines Center 725-4500
Department of Interior
5629 Minnehaha Avenue South
Minneapolis, MN 55417-3099
U.S.A.
*****************************************************************





From: Fermin, Cesar :      fermin-at-tmc.tulane.edu
Date: 12 Jun 1995 14:33:26 -0600
Subject: Guidelines equip. usage

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Message-ID: {n1409154719.35362-at-postoffice.tmc.tulane.edu}

Keeping a lab clean and avoiding abuses:

I identify well with this problem because we have here at Tulane Pathology
several core labs, one of which I direct. Remedy to this problem (disease) is
not readily available but there are pain relievers:

*1) The unit must have written guidelines of adherence with penalties for
abuse, training sessions for first time users and most importantly sharing of
the responsibility for keeping facility up by: a) users (do not work) or b)
someone else (see below),

*2) The manager must be willing, able, and have back up from superior to
enforce guidelines,

*3) Access to facility must be restricted by special combination locking
device or supervision to entrance of facility. In one of our facilities we
have a push-combination that is given after a signed Interdepartmental
Transation (IT) document is available,

*4) Users (or someone above them) must pay for service or generally users do
not give a ...,

*5) Supervisor must be able to stick to his gun, and realize that his position
is first keeping the facility and then make users happy. THERE IS NOT SECOND
WITHOUT THE FIRST.

*6) Do not ask for what it has not been explained. If succint guidelines are
not available, users are probably asked daily for something new they have not
heard.

*7) I am afraid that problems like those described on abuse of facilities
emmanates from our inability to tell others when and why they screw up: a)
first children (we praise evene when they done wrong), b) second teenagers
(they are encouraged after mediocraty), and c) third adults (demand to redo
what is wrong no matter how painful is for the asker and the doe)r. Example:
when one of my children breaks a glass as she learn to wash dishes I do not
praise that she is washing dishes. The first order to business is why the
glass broke and how not to do it again.

*8) When sticking to his guns, a supervisor will intially meet a lot of
unhappy faces, but if his main objective is to keep the facility runing and
clean and not to make friends, then I do not see what the problem is on
putting the foot down.

*9) Again, we are too afraid to tell people when they screw up and most of the
times we are unwilling to point out the terrible consequences of their lousy
work and complete lack of considerations for others. IT HAPPENS THAT TO DO
THE RIGHT THINGS DO NOT ENTER TO BRAIN BY OSMOSIS!

*10) Bottom line: My Dads uneducated moto: If they do not give a ...,
neither do I, and of course my boss must agree to it too!

I hope that this help,

******************************************************************
*Cesar D. Fermin, Ph.D \|T|/ Fax (504) 587-7389 *
*Tulane Medical School /|M|\ Voice Mail (504) 584-2618 *
*Pathology/SL79 \|C|/ Secretary (504) 584-2436 *
*New Orleans La 70112-2699 /|*|\ Lab/Techn. (504) 584-2521 *
* {Fermin-at-tmc.tulane.edu} Dir. Morphological Services *
******************************************************************





From: BARRY PYLE :      umbbp-at-msu.oscs.montana.edu
Date: Mon, 12 Jun 1995 13:56:31 MDT
Subject: Epifluorescence

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June 12, 1995

Does anyone in the U.S. know which company(ies) market Citifluor mounting fluid
for epifluorescence microscopy?




From: Patty Jansma :      plj-at-manduca.neurobio.arizona.edu
Date: Mon, 12 Jun 1995 13:37:18 -0700 (MST)
Subject: Re: Epifluorescence

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Ted Pella carries Citifluor mounting media.(cat. 19470).
P.O. Box 492477
Redding, CA 96049

Patty Jansma Tel:602-621-6671
plj-at-manduca.neurobio.arizona.edu
Arizona Research Labs Division of Neurobiology
University of Arizona


On Mon, 12 Jun 1995, BARRY PYLE wrote:

} June 12, 1995
}
} Does anyone in the U.S. know which company(ies) market Citifluor mounting fluid
} for epifluorescence microscopy?







From: dlmedli-at-california.sandia.gov (medlin douglas l)
Date: Mon, 12 Jun 1995 15:50:25 -0700
Subject: file transfer:TN 5500 to Macintosh

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We have an old Tracor Northern 5500 EDS analysis system from which
we are trying to transfer files (through the serial port) to a Macintosh
using the Xi program on the 5500 and Versaterm on the Mac. Has anyone had
similar experience (e.g. I'm interested in the proper pin connections and
software configurations, information our Noran service reps have not
been able to provide). Any help would be appreciated.

+---------------------------------------------+
! Douglas L. Medlin !
! Physical Properties of Materials Department !
! Mail Stop 9402 !
! Sandia National Laboratories !
! Livermore, California 94551 !
! !
! (510) 294-2825 !
! dlmedli-at-california.sandia.gov !
+---------------------------------------------+




From: Jane A. Fagerland (708) 935-0104 :      FAGERLAND.JANE-at-igate.pprd.abbott.com
Date: Mon, 12 Jun 1995 19:01:00 -0600 (CST)
Subject: slobs in the lab

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Mr-Received: by mta RANDB; Relayed; Mon, 12 Jun 1995 19:55:21 -0600
Mr-Received: by mta MCM$RAND; Relayed; Mon, 12 Jun 1995 19:55:22 -0600
Mr-Received: by mta RANDD; Relayed; Mon, 12 Jun 1995 19:55:29 -0600
Alternate-Recipient: prohibited
Disclose-Recipients: prohibited
Content-Return: prohibited

A few thoughts.....

Just as there are lots of different types of people in this world, there
are lots of different reasons for bad lab manners. And no one right way
to solve the problem.

In my experience, a heavy-handed approach works with very few people.
More often, it brings out some very creative passive/aggressive
responses and a lot of resentment. While I agree that some "unhappy
faces" are inevitable, much unpleasantness can be avoided by treating
people respectfully. Some people are simply unaware that they are making
messes or creating problems for those who come after them - these people
are easily "retrained" by telling them what the standards are.

The infuriating people are those who really don't care and who will not
respond to civil requests for them to change their lab habits. They
make life miserable for all around them, and ultimately, must be
banished from the lab.

However, I take exception to the idea that keeping a facility running
efficiently and keeping users on friendly terms are mutually exclusive.
I think a "kinder, gentler" approach is the best default mode and that
the Eastwood/Schwarzenegger approach should be reserved for those people
who don't respond to forthright requests. (This is not to say that I
haven't personally wanted to remove body parts from some recalcitrant
slobs, just that this is not always the best way to solve the problem!)

Jane A. Fagerland
Dept. Cellular and Microscopic Research
Abbott Laboratories
Abbott Park IL 60064






From: Dr H. Cama :      hc203-at-cus.cam.ac.uk
Date: Tue, 13 Jun 1995 09:08:33 +0100 (BST)
Subject: subscribe

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Please include my e-mail address in your mailing list.

Thanks.

H. Cama
University of Cambridge
UK




From: rsartore-at-arl.mil (Sartore, Richard G.)
Date: Tue, 13 Jun 1995 08:12 -0500 (EST)
Subject: Re: file transfer:TN 5500 to Macintosh

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We have successfully tranferred image and xray map file using Xi from the
TN5500 to
a PC and SUN station over the serial port. It is slow. A 1M image file
takes about an hour.
Transfer is in text or ASCII mode. The Tracor file format must be
translated to a more standard
format for display. A West Point Cadet, during summer visit, wrote a
Pascal/C routine to convert Tracor Northern file to PGM format for
display on monitor. The image was also
successfully printed on laser printer by converting PGM file to a
PostScript file.
Connector was wired for standard RS-232 tranfer mode.
Can get in touch with Cadet for availability of code and connector
wiring. Please let me know.
Richard Sartore
US Army Research Laboratory
AMSRL-PS-DC
Fort Monmouth, NJ 07703
908-427-2261
rsartore-at-ftmon.arl.mil






From: STEELE-at-KRDC.INT.ALCAN.CA
Date: Tue, 13 Jun 1995 08:22:51 -0500 (EST)
Subject: TEM HELP: PTA procedure request

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Would someone in the know have a minute to post a simple protocol
for negative staining with PTA?

I have a suspension of polymer "particulate", and wish to get a measure of
the size distribution. I plan to disperse onto a formvar substrate, then
shadow with Pt/C to get a basic idea of size, but would also like to try
negative staining to obtain images suitable for image analysis.

Comments would be welcome.

Thanks in advance.





From: JOHNA-at-SCI.WFEB.EDU
Date: Tue, 13 Jun 1995 09:45:20 -0400 (EDT)
Subject: Re: TEM HELP: PTA procedure request

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Hello,

I usually use a 2% or 4% solution of PTA (aqueous) and adjust the pH to 6.9
or 7.4 (specimen dependent) with KOH. The actually negative staining is as
is typical when using UA.

Good luck

John

___________________________________________________
| |
| John G. Aghajanian, Ph.D. |
| Worcester Foundation for Experimental Biology |
| 222 Maple Avenue |
| Shrewsbury, MA 01545-2737 |
| |
| Tel: 508 842-8921 ext. 147, 161 |
| Fax: 598 842-9632 |
| JOHNA-at-sci.wfeb.edu |
| |
|_________________________________________________|





From: Facility Manager (Todd Voiles) and
Date: Tue, 13 Jun 1995 10:26:09 -0400
Subject: All the responses to "Getting users to Use equip"

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Message-Id: {9506131525.AA14838-at-unlinfo.unl.edu}
X-Sender: tvoiles-at-129.93.1.11
X-Mailer: Windows Eudora Version 1.4.4
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

WOW,

This must be a real sore point for alot of managers out there, it's good
to know I'm not alone in my misery.

After 31 responses (a vertitable deluge for my underused account) the
general consensus is that I need to grow some teeth and wear some
bigger boots....steel toed would be appropriate.

One responder put it perfectly......"Most good managers learn to be
Tough n' Nice not Nice n' Tough.

Thanks for all the good advice.....I've finished writing a new use policy for
our lab (which follows at the bottom). I would like some feedback on it.....
is it to nice, to mean, uninforcable?

Anyone can feel free to use any or all of it for your own purposes (for a
small copyright fee of course ; ) }.

Thanks.

What follows is the proposed new use policy for our laboratory-




Memorandum

To: Persons using the Central Facility for Electron Microscopy
and its equipment


Re: The new policy concerning the Specimen Preparation room

Due to the current state of Specimen Preparation room (a mess)
a new policy has been implemented and will go into effect at the
end of June when the policy is finished and avialable for review.
All persons wishing to use the specimen preparation room and its
equipment will first have to notify Facility Manager so he can
detail this new policy. After the user agrees to the new policy he
or she is free to use the equipment in the appropriate manner.

**Any persons found in the specimen preparation room without first
having talked to Facility Manager will be expelled and lab
privileges revoked until further notice.**


The new policy is as follows:


Samples:

*All samples or materials brought into the laboratory or used by
users must be kept in their respective drawers or areas at all
times when not being actively being worked on and an MSDS is required
to be on file at all times.

*Any samples left unattended and unmarked for more than 2 hours may
be seized by Facility Manager and held until picked up by the user they
belong to. This action will be deemed a violation.


Equipment Use:

*All users wishing to use a specific piece of equipment will need
to be trained in its operation by the Facility Manager or one of
several officially appointed student instructors.

*All users will follow either the manufacturers manual or the
instruction sheets provided by Facility personnel. Deviation
from these instructions will be deemed a violation.

*All users must fill out use logs for equipment when operating it,
failure to do so will be deemed a violation.

*Any users damaging or finding equipment damaged or any lack of
supplies should contact the Facility Manager immediately. Failure
to report damage will be deemed a violation.

Consequences of Violation:

*Upon the first violation a warning will be issued and the user
will be restricted to working only during normal lab hours (8-5).

*Upon the second instance an additional warning will be issued and
the user will be restricted to working only when Facility Manager
is present in the laboratory and a notice will be sent to their
advisor and the Facility Director.

*Upon the third violation all laboratory privileges will be revoked
and a meeting with the user and their advisor and the Facility
Director will need to be arranged to resolve the problem.


These restrictions will be lifted when the user sufficiently
demonstrates that he or she understands the problem and works to
solve it.


A full copy of the new policy is in development and will be made
available in the Facility when it is completed (around the end of
June). If you have any questions regarding this policy or its
elements please contact the Facility Manager (Todd Voiles) at
2-8762 or tvoiles-at-unlinfo.unl.edu.




Todd Voiles
Facility Manager
Central Facility for Electron Microscopy
Center for Materials Research and Analysis

University of Nebraska at Lincoln

tvoiles-at-unlinfo.unl.edu





From: Greg :      GREG-at-umic.umic.sunysb.edu
Date: Tue, 13 Jun 1995 11:52:48 EST5DST
Subject: Lab Mess

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This sounds like a good way to go, Mr. Voiles.
If the policy is followed, word will spread and the abuse
should go away. I work at a core facility and "no one" is
left unsupervised and there is no mess.

Good luck!

Gregory Rudomen
University Microscopy Imaging Center
S.U.N.Y. at Stony Brook
Greg-at-umic.umic.sunysb.edu





From: BARRY PYLE :      umbbp-at-msu.oscs.montana.edu
Date: Tue, 13 Jun 1995 10:25:19 MDT
Subject: Epifluorescence - Thanks!

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June 13, 1995

Thanks to all who sent info on sources of Citifluor and Biomount for
epifluorescence microscopy. It's great to get information like this so easily!

Barry Pyle, Department of Microbiology, Montana State University-Bozeman




From: Beverly E Maleeff :      Beverly_E_Maleeff%notes-at-sb.com
Date: 13 Jun 95 12:55:28 EDT
Subject: Multi-User EM Facilities...again

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Having seen the messages about EM facility etiquette, or the lack thereof, I'd
like to remind everyone of a session at the upcoming MSA meeting that may be of
interest.

The MSA Technologists' Forum (TF) sponsors a roundtable discussion each year at
the Annual Meeting. This year's topic is, "EM Facility Management: Survival
Part III - What Works and What Doesn't". It is a continuation of the
discussions held at the last two meetings, and the TF offers a forum (pun
intended) for just this kind of ongoing dialogue, be it at this session, or at
our exhibit booth throughout the meeting week. If you plan to be in Kansas
City, I invite you on behalf of the TF to join us and bring these kinds of
concerns for discussion. More details about the session will be available in
the MSA meeting program.

Regards,
Bev Maleeff, TF Chairman-Elect

SmithKline Beecham Pharmaceuticals
Toxicology-US, UE0462
709 Swedeland Road
King of Prussia, PA 19406
610/270-7987
610/270-7202 fax
maleeffbe-at-sb.com or Beverly_E_Maleeff%Notes-at-sb.com





From: Michael Rock :      merock-at-u.washington.edu
Date: Tue, 13 Jun 1995 11:16:53 -0700 (PDT)
Subject: Re: Controlling People in User Laboratories

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I agree!
as a microscopy lab manager we (myself included) have the wonderful
opportunity to open the eyes and imaginations of bright eager young
people. most likely the equipment is not our own, and is usually covered by
service contract, lighten up. students will make mistakes (just like us,
who have years...decades of experience) . this philosophy in no way
gives them the right to rage wrecklessly through the lab, but few that I
work with ever do that. most of the "accidents" were just that, and I
take most of the responsibility for not preparing the students for the
possible outcomes. keep your sense of humor life is short. teach the
student how to avoid damaging the equipment (operator manuals &
individual instruction), encourage them to experiment, and employ
the appropriate security for the facility.
be a teacher, not a policeman!
-Mike Rock
U.W. Zoology Dept.

On Tue, 13 Jun 1995 JMARDINLY-at-IMO.intel.com wrote:

} My sympathies to those of you out there who have no sense of humor. It
} is important to have a sense of humor when managing user laboratories. For
} those who replied to my suggestion of hiring Arnold Schwartzenegger and
} Clint Eastwood as special assistants as too heavy handed, and did not recognizeit as sarcasm, get a life. Managing a user laboratory is akin to managing
} the human condition, and that is a most challenging task. You will have a
} hard time stopping people from being themselves, and you cannot stop people
} from inventing insane new ways to misuse equipment. Just teach the best you
} can and grin and bear it when the results come out different from what you had
} hoped for.
} John Mardinly
} Intel Materials Technology
}




From: STEELE-at-KRDC.INT.ALCAN.CA
Date: Tue, 13 Jun 1995 08:22:51 -0500 (EST)
Subject: TEM HELP: PTA procedure request

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Message-Id: {1995Jun13.111734.1158885706-at-ms.sjdccd.cc.ca.us}
To: MICROSCOPY-at-aaem.amc.anl.gov (microscopy list),
STEELE-at-krdc.int.alcan.ca (STEELE)

Depending on exactly what type of polymer it is you might want to stain it
with ruthenium tetroxide or osmium tetroxide if the neg stain does not
work.

When using the PTA: You might also want to try to adjust the pH of the PTA
to 5.0 as sometimes PTA works as a negative or positive stain depending on
exactly what pH and ionic condition the specimen is
in.
_______________________________________________________________________________



Would someone in the know have a minute to post a simple protocol
for negative staining with PTA?

I have a suspension of polymer "particulate", and wish to get a measure of
the size distribution. I plan to disperse onto a formvar substrate, then
shadow with Pt/C to get a basic idea of size, but would also like to try
negative staining to obtain images suitable for image analysis.

Comments would be welcome.

Thanks in advance.



______________________________________________________________________________
San Joaquin Delta College World Wide Web:http://www.sjdccd.cc.ca.us
5151 Pacific Avenue email:webmaster-at-ms.sjdccd.cc.ca.us
Stockton, CA 95207
general information:(209) 474-5151 or FAX-at-(209)474-5600





From: Michael Rock :      merock-at-u.washington.edu
Date: Tue, 13 Jun 1995 15:07:05 -0700 (PDT)
Subject: magneto optical disc reader

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i am looking for a lab in the great northwest who has a SONY magneto
optical disc reader. one of our researchers went to san diego, aquired
some confocal data, and copied it on a SONY worm disk.
we unfortunately for her, have a panasonic worm dirve. the disk holds
approx. the same amt of data, but will not fit into the drive. i need to
down load this data, does any one here inseattle have a SONY worm drive ?
if so please contact me soon.
tia
merock-at-u.washinton.edu




From: bozzola-at-siu.edu (John. J. Bozzola)
Date: Tue, 13 Jun 1995 17:44:52 -0600
Subject: EPMA: Fly ash trace elements

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Mime-Version: 1.0
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Dear Analysts-
One of our researchers wishes to map trace elements found in fly ash.
Anyone have any good references or protocols they would share with us? I
assume that the samples should be enrobed in resin, polished, etc. but
details would be appreciated. Thank you very much.

John J. Bozzola
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
Phone: 618-453-3730
Fax: -2665
Email: bozzola-at-siu.edu OR bozzola-at-qm.c-cem.siu.edu






From: bjg-at-uniwa.uwa.edu.au (Brendon J. Griffin)
Date: Wed, 14 Jun 1995 10:05:59 +0800
Subject: What is the ideal EM centre profile

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Dear all,

At the University of Western Australia we have a Centre for Microscopy and
MIcroanalysis. Currently we have 3 TEMs, 5 SEMs (inc FESEM, ESEM) and a
confocal together with a range of spec prep facilities. We run quarterly
2-3 day short courses in all aspects of EM and confocal. We have a Director
and 2 academics (covering materials, geoscience and biological), a
secretary and 5.5 technical support staff. Users drive the instruments
themselves following successful attendance at one of the training courses.
There are currently no charges as we are funded by a group of Faculties -
the budget is minimal and ~30% is raised by consulting and competitive
grants by the academics. Major equipment for the last five years has been
gained through national competitive funding with seed funding from the
University. We also act in a regional capacity supporting EM research from
two other local Universities.

As usual we are under audit.

I would appreciate greatly any comment on:

ideal and actual centre profiles - what equipment is actually needed for a
cross-campus or single discipline facility

funding strategies - charge rates : do you charge? what does it cost to
charge? is the charging a full cost recovery? where does the rest come
from?

staff profile - actual and ideal: particularly academic or technical or
mixture - note here this may be historically controlled, ie if you have
academics in a centre then the user groups may not develop the expertise
themselves

equipment replacement policies - is there a plan or is it serendipidous as
opportunities arise?

equipment maintenance policies - eg in-house or service contract



I am very concerned at the difficulties many EM facilities are having
world-wide and plan to try and present a review of trends apart from using
the data ourselves.

All data will be kept in confidence w.r.t. location/people etc but I would
need some ID to verify original data.


If I get enough feedback I will put a draft/summary back through Nestor.
Thanks in advance

Happy kangaroos


Brendon
Brendon J. Griffin
Centre for Microscopy and Microanalysis
The University of Western Australia
Nedlands, WA, AUSTRALIA 6907
ph 61-9-380-2739 fax 61-9-380-1087





From: dietmar.reiter-at-uibk.ac.at (Dietmar Reiter)
Date: Wed, 14 Jun 1995 12:09:38 +0100
Subject: Imaging gray cataract dimmed eye lenses

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Dear bio-medical Imagers,

any experiences with confocal imaging of cataract lenses to get a
representation of the blurred area in 3D? Is confocal imaging possible at
all?

Thanks for hints, -Dietmar-


+++ Dietmar Reiter, Dept. of Zoology and Limnology
+++ University of Innsbruck
+++ Technikerstrasse 25, A-6020 Innsbruck, Austria
+++ phone: (43)-512-507 ext. -6170, fax ext. -2930






From: EMLAB-at-opus.mco.edu
Date: Wed, 14 Jun 1995 08:52:47 -0500 (EST)
Subject: Re: TEM HELP: PTA procedure request

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From: mrc-at-vax.ox.ac.uk
Date: Wed, 14 Jun 1995 14:30:36 +0100
Subject: subscribe

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Sender: mrc-at-vax.ox.ac.uk





From: sulovsky-at-sci.muni.cz (Petr Sulovsky)
Date: Wed, 14 Jun 1995 10:18:09 -0400
Subject: CamScan phone/e-mail?

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Message-Id: {199506140820.AA21400-at-elanor.sci.muni.cz}
X-Sender: sulovsky-at-elanor.sci.muni.cz
X-Mailer: Windows Eudora Version 1.4.3
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Hello Microscopists,

could someone help me with current phone number of CamScan (Mr. Holliday's,
preferably), or (better), their e-mail code (they once had some)?

Thanks

Dr. Petr Sulovsky
Dept. of Mineralogy, Petrology and Geochemistry
Faculty of Science
Masaryk University, Brno
Postal address: Kotlarska 2
CZ 611 37 Brno
Czech Republic
Fax +42-541211214, phone +42-541129231
E-mail: sulovsky-at-sci.muni.cz





From: richard.easingwood-at-stonebow.otago.ac.nz (Richard Easingwood)
Date: Wed, 14 Jun 1995 14:38:05 +1100
Subject: Image processing tutorial software?

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Does anyone know of any software package which is designed to introduce
people to digital image processing, perhaps a sort of tutorial which would
guide one through some of the basic tools such as filters, edge
enhancement, equalisation, contrast manipulation, etc?
Thanks in anticipation,
Richard E

Richard Easingwood
South Campus Electron Microscope Unit
Otago Medical School
PO Box 913
Dunedin
NEW ZEALAND

Telephone: 64-03-479 7301
Facsimile: 64-03-479 7254

"The southernmost electron microscope unit in the world"






From: cbarba-at-eucmax.sim.ucm.es (andy johnson)
Date: Wed, 14 Jun 1995 18:15:37 +0100
Subject: Image processing tutorial software?

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subscribe






From: Wan Junzuo :      junzuo-at-mcmail.CIS.McMaster.CA
Date: Wed, 14 Jun 1995 12:43:42 -0400 (EDT)
Subject: unsubsribe

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I would like to unsubscribe, please.

junzuo wan




From: diamond-at-chem.psu.edu (John V. Badding)
Date: Wed, 14 Jun 1995 12:41:56 -0400
Subject: unsubsribe

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Can anyone advise me where we could go to perform EELS measurements on a
TEM on some carbon samples? preferably within several hours drive of Penn
State in central Pennsylvania. We have done these measurements on a STEM,
but want to have better simultaneous diffraction and EELS capabilities.
Thank you.
John Badding






From: GVKM07A-at-prodigy.com (DR CHARLES A GARBER)
Date: Tue, 13 Jun 1995 23:40:28 EDT
Subject: EPMA: Fly ash trace elements

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-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --

On June 13, John Bozzola wrote about fly ash particles:

1] We have gotten or best EDS data on thin sections via TEM. We use
our SPI Supplies "Materials Science" diamond knife and our SPI-Pon 812
Resin system. The sections seem also to be suitable for RBS analysis.
Vacuum embedding is important and then you have to be careful that the
largest particles are not "pulled out" of the section producing a non-
representative picture of the sample as a whole.

2] If a TEM is not going to be used, then mounting the particles using
our "Tacky Dot" slides and then embedding and polishing down to cross
sections would give you a nice orthogonal array of cross sectioned and
polished particles which makes the EPMA a "breeze" compared to what
would otherwise be the case.

Chuck

Charles A. Garber, Ph. D.
PRESIDENT
STRUCTURE PROBE, INC.
PO Box 656
West Chester, PA 19381-0656

Ph: (610) 436-5400
FAX:(610) 436-5755
e-mail: GVKM07A-at-prodigy.com





From: JOHNA-at-SCI.WFEB.EDU
Date: Wed, 14 Jun 1995 11:46:08 -0400 (EDT)
Subject: LR Gold

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Message-Id: {9506141834.AA28863-at-riker.ml.wpafb.af.mil}

G'Day Folks,

I have been using LR White for years, both for straight morphology and
immunocytochemistry, but have not used LR Gold. I know someone out there
is using LR Gold for light & EM immunocytochemistry since I seem to
remember reading something about it in this forum.

I'd appreciate it if you folks could answer a few questions. I'd like to
give LR Gold a try for light and EM IMC and in situ hybridization.

1. If using lightly fixed tissue (4% PA + 0.1-0.2% GA) does need
to add PVP to the methanol? Can you use ethanol instead of methanol? The
infiltration times listed in the LR Gold brochure are quite long and
haven't changed since they first started selling the stuff; is it that
difficult to get this stuff into tissues and cultured cells or were they
just being extremely cautious when they wrote up the protocol.

2. Can L.R. Gold be heat polymerized in an oven?

3. Any other helpful hints?

Thanks very much in advance. See you in K.C.

John

___________________________________________________
| |
| John G. Aghajanian, Ph.D. |
| Worcester Foundation for Experimental Biology |
| 222 Maple Avenue |
| Shrewsbury, MA 01545-2737 |
| |
| Tel: 508 842-8921 ext. 147, 161 |
| Fax: 598 842-9632 |
| JOHNA-at-sci.wfeb.edu |
| |
|_________________________________________________|





From: Michael Boucher :      BOUCHER-at-tcrca.usbm.gov
Date: Wed, 14 Jun 1995 14:29:10 CST
Subject: NORAN Voyager (WDS?) users group

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Another user recently contacted me by e-mail about problems with his
Voyager system, describing exactly my recent problems with it too.
I would like to get a e-mail/phonel list of Voyager users, but particularly
WDS users, since that system is still in development and version 3 is
due out (to be delivered to me anyway) in late August. Please e-mail
me directly if you are interested in starting up a "user's group"
that would share help and advice by phone and e-mail; not burdening
the microscopy listserver with our traffic unless it seems to be of
general interest or new people ask for help regarding Voyager. Any
takers?
Mike
*****************************************************************
Michael L. Boucher Sr. Boucher-at-TCRCA.USBM.GOV
Geology-Mineralogy/Chemistry Labs Ph 612-725-4614
Twin Cities Research Center Fax 612-725-4527
U.S. Bureau of Mines Center 725-4500
Department of Interior
5629 Minnehaha Avenue South
Minneapolis, MN 55417-3099
U.S.A.
*****************************************************************





From: bjg-at-uniwa.uwa.edu.au (Brendon J. Griffin)
Date: Wed, 14 Jun 1995 15:21:55 +0800
Subject: What is the ideal EM centre profile

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Message-Id: {199506140713.PAA26276-at-uniwa.uwa.edu.au}

Dear all,

At the University of Western Australia we have a Centre for Microscopy and
MIcroanalysis. Currently we have 3 TEMs, 5 SEMs (inc FESEM, ESEM) and a
confocal together with a range of spec prep facilities. We run quarterly
2-3 day short courses in all aspects of EM and confocal. We have a Director
and 2 academics (covering materials, geoscience and biological), a
secretary and 5.5 technical support staff. Users drive the instruments
themselves following successful attendance at one of the training courses.
There are currently no charges as we are funded by a group of Faculties -
the budget is minimal and ~30% is raised by consulting and competitive
grants by the academics. Major equipment for the last five years has been
gained through national competitive funding with seed funding from the
University. We also act in a regional capacity supporting EM research from
two other local Universities.

As usual we are under audit.

I would appreciate greatly any comment on:

ideal and actual centre profiles - what equipment is actually needed for a
cross-campus or single discipline facility

funding strategies - charge rates : do you charge? what does it cost to
charge? is the charging a full cost recovery? where does the rest come
from?

staff profile - actual and ideal: particularly academic or technical or
mixture - note here this may be historically controlled, ie if you have
academics in a centre then the user groups may not develop the expertise
themselves

equipment replacement policies - is there a plan or is it serendipidous as
opportunities arise?

equipment maintenance policies - eg in-house or service contract



I am very concerned at the difficulties many EM facilities are having
world-wide and plan to try and present a review of trends apart from using
the data ourselves.

All data will be kept in confidence w.r.t. location/people etc but I would
need some ID to verify original data.


If I get enough feedback I will put a draft/summary back through Nestor.
Thanks in advance

Happy kangaroos


Brendon
Brendon J. Griffin
Centre for Microscopy and Microanalysis
The University of Western Australia
Nedlands, WA, AUSTRALIA 6907
ph 61-9-380-2739 fax 61-9-380-1087





From: rybicka-at-acsu.buffalo.edu
Date: Wed, 14 Jun 1995 12:32:18 -0400
Subject: glycogen ultrastructure

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Hi All Those Interested in Glycogen,

There is a short review in MICROSCOPY TODAY, October, 1994 entitled
"GLYCOGEN GFRANULES REVISITED". It explains that glycogen in the tissue
contains covalently bound protein. The event is well established in
biochemistry, but still misinterpreted in EM. Since this protein contains
enzymes involved in glycogen metabolism, the structures composed of
glycogen-protein complex were considered as cellular organelles and called
glycosomes in 1968 ! So called "glycogen granules" stainable with uranium
and lead represent protein component (enzymes) attached to glycogen.
Glycogen itself remains invisible after routine staining with uranium and
lead salts because it does not form ionic bonds. Apparent removal of
"glycogen granules" by uranyl acetate (UA) or other acids used before
tissue dehydration, is in fact the removal of protein component. It appears
because the bond of protein to glycogen is sensitive to the change in pH
(traditional purification of glycogen is performed by the treatment with
strong alkali or acids). The removed soluble protein is presumably washed
out during dehydration. Glycogen appears as small (3 nm) particles which
normally form (20-30nm) aggregates attached to protein. Glycogen is not
fixed per se, but it is stabilized due to the fixation of the bound
protein. After removal of protein by acidic treatment, glycogen particles
floate freely in the cell forming large irregular clumps. In routinely
stained tissue these clumps appear as white spots which may even suggest a
poor plastic polymerization. However, histochemical staining (Thiery
technique) shows that these spots are composed of the clumps of 3 nm
particles of glycogen. First EM study of the subject was: RYBICKA, K.
1979. Virchows Archiv B. Cell Pathol. 30, 355-47. Other references are
included in the mentioned review in MICROSCOPY TODAY. If you cannot get it,
please, let me know, and I will write you more details and references.

Considering present status of biochemical knowledge about glycosomes,
microscopic and molecular biology techniques seem to offer a good field for
further research.

Good luck,

Krystyna Kielan Rybicka
SUNY at Buffalo
Physiology / Neurobiology
Buffalo, NY

---
-------------------------------------------------------------------------
email address rybicka-at-acsu.buffalo.edu
phone number (lab) 716 829 3575
-------------------------------------------------------------------------





From: EvexAnalyt-at-aol.com
Date: Wed, 14 Jun 1995 19:35:19 -0400
Subject: Re: Image processing tutorial software?

Contents Retrieved from Microscopy Listserver Archives
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Yes We have available for you a demo version of ImagePro please respond to

Evex Analytical Instruments
PO Box 630
Princeton, NJ 08542
USA
(908)874-3800
EvexAnalyt-at-aol.com




From: EvexAnalyt-at-aol.com
Date: Wed, 14 Jun 1995 19:43:16 -0400
Subject: Re: EDS: file transfer from a TN5500 to a Mac

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Evex Analytical Instruments has developed the VIDX INTERFAZER

The VIDX INTERFAZER comes in 2 models the operate on a PC.

One unit is transfers the image instanly-live to the PC for low resolution
Images or Dot Mappings

The other uses transfers the images & Spectra at a slower pace with greater
resolution.

For more information please contact

Evex Analytical Instruments
PO Box 630
Princeton, NJ 08542
(908)874-3800




From: EvexAnalyt-at-aol.com
Date: Wed, 14 Jun 1995 19:44:30 -0400
Subject: Re: EDS: file transfer from a TN5500 to a PC

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Evex Analytical Instruments has developed the VIDX INTERFAZER

The VIDX INTERFAZER comes in 2 models the operate on a PC.

One unit is transfers the image instanly-live to the PC for low resolution
Images or Dot Mappings

The other uses transfers the images & Spectra at a slower pace with greater
resolution.

For more information please contact

Evex Analytical Instruments
PO Box 630
Princeton, NJ 08542
(908)874-3800




From: EvexAnalyt-at-aol.com
Date: Wed, 14 Jun 1995 19:51:46 -0400
Subject: Tracor/Noran Kevex, PGT USER'S GROUP Q & A

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Please e-mail me directly if you are interested in a USER'S GROUP
that would share advice by phone and e-mail





From: MicroToday-at-aol.com
Date: Wed, 14 Jun 1995 22:54:44 -0400
Subject: Manufacturers

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Group -
As an "ex-manufacturer" I am particularly interested in the "aroma" presented
about manufacturers as carried in this media. As an example I refer to a
recent note regarding the Noran/TN 5500 by Douglas Medlin at Sandia which
read "I'm interested in the proper pin connections and software
configurations, information our Noran service reps have not been able to
provide".
Since I happened to had a "responsible" position at TN when this system was
sold, I looked into the matter. The facts are that the pin connections were
supplied to Sandia some 3 months ago. And, with Noran's offer to fax another
set, they found the previous set. Also - a very qualified Noran service tech
(from the home office) recently spent 3 days on-site checking out the system
- with the conclusion that the problem lies in the Mac PC.
Two points in conclusion:
1) I suggest that participants in this listserver be very careful in their
reference to manufacturers or suppliers - be they positive or negative. The
reason being that such, from folks like you all, do logically tend to carry
much weight.
2) I happen to be a bit proud of the work/products/service that TN provided
when I was there. And I have no comment on the current support, etc. of any
of the EDS companies.
Cheers,
Don Grimes, Microscopy Today




From: diamond-at-chem.psu.edu (John V. Badding)
Date: Thu, 15 Jun 1995 08:53:40 -0400
Subject: EELS on the East Coast

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I sent this message out previously without any subject. I apologize for
the repeat for those of you who have already read it.

Can anyone advise me where we could go to perform EELS measurements on a
TEM on some carbon samples? preferably within several hours drive of Penn
State in central Pennsylvania. We have done these measurements on a STEM,
but want to have better simultaneous diffraction and EELS capabilities.
Thank you.
John Badding






From: CHAFFEYN :      NIGEL.CHAFFEY-at-bbsrc.ac.uk
Date: Thu, 15 Jun 1995 09:36:32 -0500
Subject: Digitising pad

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X400-Received: by mta bottom.ctd.anl.gov in /PRMD=ANL/ADMD= /C=US/; Relayed;
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Dear All,

Can anybody help a colleague of mine? He is trying to locate a source
for a 'Calcomp 2000 digitising pad and cursor' to fit a VIDS II image analysing
system on an Apple IIe. The previous pad plus cursor has disappeared, but was
obtained from Analytical Measuring Systems Ltd of Saffron Walden, Essex (UK).
He has been unable to trace the original company. Therefore:
can anybody suggest a source for the required item?
does anybody have one that is surplus to their requirements, which
could be 'acquired', or,
can anybody tell me if the original company is trading under another
name?

Many thanks in advance

Nigel.Chaffey-at-BBSRC.AC.UK..




From: emccjb-at-ttuhsc.edu (Charles J. Butterick)
Date: Thu, 15 Jun 1995 09:36:32 -0500
Subject: MSA meeting

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To: MICROSCOPY-at-AAEM.AMC.ANL.GOV

Greetings,
Would someone please send MSA's address, phone number, email and/or
fax so that I can get details on August's meeting? Thanks.



Charles J. Butterick (Chuck)
Electron Microscopy Center
Department of Cell Biology
and Biochemistry
Texas Tech University Health
Sciences Center
3601 4th Street
Lubbock, Texas 79430

vox (806) 743-1633
fax (806) 743-1219
email emccjb-at-lubb.ttuhsc.edu or
chuck-at-micron1.lubb.ttuhsc.edu






From: Eugene Bluhm :      bluhm-at-umr.edu
Date: Thu, 15 Jun 1995 10:39:39 -0600 (CDT)
Subject: MSA meeting

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subscribe




From: Jay Jerome :      jjerome-at-isnet.is.wfu.edu
Date: Thu, 15 Jun 1995 11:38:41 -0400 (EDT)
Subject: EM AND LM IMMUNOSTAINING: Ab to FITC

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I have a client who wishes to immunostain for FITC. He would like to do
this at the light microscope level in both parafin and fixed,
cryosectioned material. He would also like to do this at the EM level by
pre-embed immunostaining.

My questions are:

1. Any recommendation on which of the Anti-FITC anti-bodies work well (or
don't work) for immunhistochemistry? [species the antibody was raised in
is not limiting at this point]
a. Parafin embedded material?
b. Crosections?
c. Fixed, but unembed material?

2. Can anyone provide a good reference (I have a couple of papers but the
methods are sketchy) or protocol for any of the three above staining
situations with Anti-FITC? Concentrations, times, etc?

3. Are there any unusual pitfalls to worry about? We have considerable
experience with immunostaining, so have the basics down.

I appreciate any help y'all can provide. Thanks

Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-isnet.is.wfu.edu *
**************************************************************





From: sbianchi-at-dbag.unifi.it (Stefano Bianchi)
Date: Thu, 15 Jun 1995 18:05:25 +0100 (MET)
Subject: Kurta Penmouse for Macintosh

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Dear All,

Can anybody help a colleague of mine? The Kurta Penmouse for
Macintosh best run on the Mac with 2 Serial Port. This graphic tablet not
work on Mac Powerbook 150 because it have only one serial port?
This tablet required a specific driver to run on PW 150?

Many thanks in advance
Stefano Bianchi sbianchi-at-dbag.unifi.it
Dept. of Animal Biology
Univ. of Florence
Firenze, ITALY






From: Goldmarker-at-aol.com
Date: Thu, 15 Jun 1995 12:22:02 -0400
Subject: Coated grids for Acrylics?

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Why is it necessary to use coated grids for acrylic embedding resins, but not
for epoxy resins?

What is the mechanism?

I would appreciate your help.

Thanks, Donald P. Cox




From: hunt-at-msc.cornell.edu
Date: Thu, 15 Jun 1995 13:06:58 -0400 (EDT)
Subject: silicon microtoming diamond knife

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Reply-To: hunt-at-msc.cornell.edu

Hi,

Does sombody know if one can cross-section a 25 micron

thick silicon piece with a diamond knife (microtoming).

Will that damage or dull the knife?

Thanks

jandt-at-msc.cornell.edu




From: hukee.margaret-at-mayo.EDU (Marge Hukee)
Date: Thu, 15 Jun 1995 13:46:39 +0200
Subject: Re: uranyl acetate

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Dear microscopists;
Just a note to update the uranyl crisis that we had encountered earlier.
Stacie Kirch was extremely helpful in clearing up the problem with uranyl
that was insoluble in water. We have tested a new batch supplied by both
EMS and Ted Pella and found that both suppliers now have uranyl that is
soluble at the 2% level. This is a vast improvement over previous lots of
uranyl.
Marge

Marge Hukee
EM Core Facility hukee.margaret-at-mayo.edu
Mayo Foundation (507) 284-3148
----------------------------------------------------------------------------
--






From: jester-at-crnjjsgi.swmed.edu (James V. Jester)
Date: Thu, 15 Jun 1995 11:09:44 -0500
Subject: Using same species AB

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Sandy Masur last week had asked if any one knew how to do
co-localization with antibodies from the same species. I have
just come across an ad from Zymed for HistoMouse-SP which
supposedly contains a blocker for endogenous rodent IgG which
will enable one to use either rat or mouse IgG to localize
antigens. Perhaps a similar approach can be used to block
antigenic sites on the first antibody prior to application of
the second. has anyone used this HistoMouse-SP Kit. In the
long run I would think it would be preferable to make appropriate
antibodies in different species. I hope this might be of some
help.

_____________________________________________________________________
| | |
| James V. Jester | Dept Ophthalmology |
| jester-at-crnjjsgi.swmed.edu | UT Southwestern Medical Center |
| TEL (214)648-7215 | 5323 Harry Hines Blvd |
| FAX (214)648-2382 | Dallas, TX 75235-9057 |
|__________________________________|__________________________________|





From: Mr Herbert Mohr :      H.Mohr-at-ph.surrey.ac.uk
Date: Thu, 15 Jun 1995 18:50:44 +0100
Subject: SEM, EBIC, beam current measurements

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I do EBIC (Electron beam induced current) measurement on semiconductors, like GaAs,
InP, and other. One important parameter is the beam current in the micrcoscope. We
have an Oxford S250 with a normal tungsten cathode in our central microscopy facility.
The beam current is measured with a small Faraday cup and an external current amplifier.
The current is in the range of 0.1 to 5nA. But the beam is not always stable and I get
large fluctuations and/or drift in the current, which can be as big as 20%. I use the spot
size control, in order to get a certain beam current. But for the measurement on the
sample a fairly stable current is required.
Does anyone know, what one can expect as a good stable beam current and how to
achieve it? Or what might be wrong with the microscope that I experience such large
fluctuations.

cheers
Herbert Mohr
--------------------------------------
Science is the enemy of illusion.
-----------------------------------------------------------------------------
Herbert Mohr
Department of Physics, Optoelectronic and Devices group
University of Surrey
GU2 5XH
United Kingdom

e-mail: H.Mohr-at-ph.surrey.ac.uk
tel. : 01483/259403

SEM/EBIC - Monte Carlo simulation
------------------------------------------------------------------------------




From: cook-at-aaem.amc.anl.gov (Russell E. Cook)
Date: Thu, 15 Jun 1995 14:28:59 -0600
Subject: Re: old EDAX systems

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Message-Id: {v01510102ac0648556a38-at-[146.139.72.78]}
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Concerning Scott Walk's recent comment (see below), EDAX has a program
called EDCVRT for its old PDP11 systems such as the PV9900: it translates
EDAX's EDS spectral files into ASCII format. Alan Sandborg of EDAX gave us
a copy 2-3 years ago. Also, the Fortran program NTRANS which Nestor
Zaluzec developed several years ago to translate various EDS spectral file
formats is still available from the anonymous ftp site WWW.AMC.ANL.GOV (IP
number 146.139.72.10). It may not be completely debugged: I had to
rewrite a small portion of it for the EDAX PV9900. I have taken Nestor's
code for the EDAX 9900 and modified it so that you are able to do batch
translations of EDAX's EDS files interactively. The ASCII files can then
be ported to a Mac or a PC over a serial line by a simple, albeit slow,
method. These text files have a MSA header and the data is in a single
column.

} Comment #3: With as many pieces of older EDS equipment that have been out
} there, the manufacturers should have written and distributed software to
} translate their spectra, data files, and image files to a standard format for
} both Mac and PC's. In fact, Nestor took the lead several years ago with
} establishing a format for EDS spectra, but no support from the manufacturers
} came about for older systems....
}
} JMHO
}
} Scott Walck

Russell E. Cook
Electron Microscopy Center for Materials Research
Argonne National Laboratory
9700 South Cass Avenue
MSD-212
Argonne, IL 60439
(708)252-7194
FAX: (708)252-4798






From: EMLAB-at-opus.mco.edu
Date: Thu, 15 Jun 1995 09:46:42 -0500 (EST)
Subject: TEM:PTA staining

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Sorry about yesterday's lost message. Concerning the procedure about
negative staining of "polymere particles" with PTA. The procedure I have used
for viral particles is as follows: 1) Float a formvar-carbon coated grid on
drop of sample for 10-20 minutes. 2) Drain all excess fluid by touching edge
of grid to blotter paper. 3) Float grid on stain for 10 seconds and drain and
air dry.
PTA is a funny negative stain. It can be used at concentrations
ranging from 0.5-3% and at pH's between 4-8. You need to do various
combinations of the above and observe which is best for your sample.
You might want to try making a grid and not stain it. There might be
enough contrast so measurements can be obtained.

Hope this helps,

Ed Calomeni
Medical College of Ohio
Toledo, OH
emlab-at-opus.mco.edu






From: bergrh-at-cc.memphis.edu (R. Howard Berg)
Date: Thu, 15 Jun 1995 17:08:24 +0600
Subject: Re: manufacturers and old equipment

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WRT Scott Walck's comment 3 (below) that was in response to Don Grime's
message, I fully agree. I talked with the Tracor Northern people several
times in an effort to get help sending TN 5500 data to my Mac. They sent
me the file format for the FLEX images but made it understood that it was
up to me to find a programmer to get the files interpreted by the Mac.
They were interested selling us a newer Tracor system-but we've got more
pressing things to spend our budget on.

Howard Berg

Comment #3: With as many pieces of older EDS equipment that have been out
there, the manufacturers should have wirtten and distributed software to
translate their spectra, data files, and image files to a standard format for
both Mac and PC's. In fact, Nestor took the lead several years ago with
establishing a fromat for EDS spectra, but no support from the manufacturers
came about for older systems. This should have been done to include detailed
cabling and interface instructions. This should have been done with minimum
cost to the users who have to live with the systems that is some cases have
been purchased by others.

JMHO

Scott Walck


R. Howard Berg, Ph.D.
Biology Department
University of Memphis
Memphis, TN, 38152
E mail: bergrh-at-cc.memphis.edu
phone: 901-678-4449 fax: 901-678-4457







From: ingen-at-Rt66.com
Date: Fri, 16 Jun 1995 06:00:40 -0600
Subject: Re: manufacturers and old equipment

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Message-Id: {9506161202.AA16896-at-Rt66.com}
X-Sender: ingen-at-rt66.com
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subscribe





From: SGKCCK-at-aol.com
Date: Thu, 15 Jun 1995 19:09:08 -0400
Subject: SILICON MICROTOMING WITH DK

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I saw your message regarding the above referenced subject. As Scott
suggested I do have some experiencing cutting this thickness of silicon.
However, it is rather lengthy and i do have some questions to ask before I
would make final recommendations. Please give me a call so we can
extensively discuss this.
I look forward to hearing from you.
Sincerely,
Stacie Kirsch
Diatome
Tel: 215-646-1478




From: anne-at-emu.su.oz.au (Anne Simpson Gomes)
Date: Fri, 16 Jun 1995 09:06:26 +1000
Subject: unsubscribe

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on holidays for a bit, please unsubscribe

thanx

Anne

Anne Simpson Gomes

EM Unit, F09
Univ of Sydney | OVE
NSW 2006 Australia lots of | UCK and
Fax: (612) 552 1967 |____ AUGHTER ASG







From: Diane Montpetit :      MontpetitD-at-EM.AGR.CA
Date: Fri, 16 Jun 1995 11:25:52 -0400
Subject: mocha image analysis overlapping globules

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Message-Id: {sfe16ae6.073-at-EM.AGR.CA}
X-Mailer: Novell GroupWise 4.1

hello everyone,

I am currently working with an image analysis system called MOCHA,

I am looking at light microscopy pictures of fat globules of varying sizes and trying to
measure their average diameter, but some of them are overlapping and I just can find
out how to get the image analysis system to take this into account...The boundary is
visible...Their is an exemple of coins touching to each other but nothing concerning
transparent objects overlapping.

I am wondering if the system is able to do what i want him to do...



Diane Montpetit
email;montpetitd-at-em.agr.ca
fax 514 773 8461
tel514 773 1105

food research center
quebec, canada






From: Matt Kizerian :      kizerian-at-ucsu.Colorado.EDU
Date: Fri, 16 Jun 1995 09:26:32 -0600 (MDT)
Subject: Where is Bio Rad/Polaron?

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In the Chemical Engineering Dept. of the University of Colorado at
Boulder we are trying to use our Bio Rad/Polaron E7400 cryostage system on
our Cambridge SEM.

Unfortunately it has not been used in a while & we need to reinstall part
of it & need documentation on its use. The PROBLEM is that we can't reach
Bio Rad ANYWHERE to request manuals. We've tried the following phone
numbers, but haven't even reached a recording at any of them:

617-864-5809
617-864-5820
1-800-524-8200

Is Bio Rad still out there? If so, where can they be reached? We're trying
to get some important lab protocols worked out & need to get this
equipment up and running ASAP. Thanks for any help!


Matt Kizerian
NSF Center for Seperations Using Thin-Films
Chemical Engineering Dept.
University of Colorado at Boulder




From: Sally Shrom :      sally-at-retina.anatomy.upenn.edu
Date: Fri, 16 Jun 1995 13:22:02 -0400 (EDT)
Subject: TEM / Bio / Uranyl Acetate

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Posted-Date: Fri, 16 Jun 1995 13:23:29 -0400

Uranyl Acetate: how hot is it?




From: Greg :      GREG-at-umic.umic.sunysb.edu
Date: Fri, 16 Jun 1995 13:26:05 EST5DST
Subject: IEM

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Does anyone have a protocol for the fixing of bone for gold labelling of
osteocytes in undecalcified bone. I am going to be doing TEM.

Greg Rudomen
University Microscopy Imaging Center
University at Stony Brook, New York
Greg-at-umic.umic.sunysb.edu





From: Greg :      GREG-at-umic.umic.sunysb.edu
Date: Fri, 16 Jun 1995 13:46:07 EST5DST
Subject: mocha

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I think you will have to crop out each globule. Then paste them into
one image and then do your measurements. I don't believe there is a
program to do what you want automaticly.

Greg Rudomen
UMIC
University at Stony Brook
Greg-at-umic.umic.sunysb.edu





From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 16 Jun 1995 14:39:33 -0400
Subject: RE- Bio-Rad Addr

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Message-ID: {n1408808702.55522-at-mse.engin.umich.edu}

Subject: Time: 2:32 PM
OFFICE MEMO RE: Bio-Rad Addr Date: 6/16/95

The 1995 list of manufacturers published by R & D Magazine has two entries
for Bio-Rad:
Digilab Div, 237 Putnam Ave., Cambridge, MA 02139.
Ph: 617-868-4330, and
Sadtler Div, 3316 Spring Garden St.,Philadelphia, PA 19104
Ph:215-382-7800
Good Luck!





From: baskin-at-biosci.mbp.missouri.edu (Tobias Baskin)
Date: Fri, 16 Jun 1995 09:17:43 -0500
Subject: SPM (any type)/plant cell walls

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Greetings,
Is there anyone out there who is doing (or who knows of someone
doing) spm on isolated plant cell walls? I am interested in this myself and
I would like to hear from anyone who has had experience with this type of
sample.

Thanks in advance,
Tobias Baskin

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
___ ____ ^ ____ _____ Tobias I. Baskin
/ \ / / \ / \ / University of Missouri
/ | / / \ / / Biological Sciences
/___ / /__ /_____\ / /__ 109 Tucker Hall
/ / / \ ( / Columbia, MO 65211 USA
/ / / \ \ / voice: 314-882-0173
/ /____ / \ \____/ /_____ fax: 314-882-0123






From: jfmjfm-at-umich.edu (John F. Mansfield)
Date: Fri, 16 Jun 1995 09:54:50 -0400
Subject: TN/Noran5500 to Mac

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This ought to be in the FAQ list!

In fact this applies to any PDP-11 based XEDS system.
In the PDP-11 put an ethernet card in the QBUS. Get a license for RT-11,
not difficult since people are tossing out PDP-11 systems left and right.
Buy a copy of ftp for RT-11 from Process Software. Run ftpd on the RT system
Run a strip of ethernet from the PDP-11 to the Mac (or PC or Unix box) and
run Fetch (or your favourite ftp client) and list the files on your pdp
hard disk and then select the ones you want and pull them over. Works very
will and you can do images too!

Contact me of line for further information.

OK?

John Mansfield
North Campus Electron Microbeam Analysis Laboratory
413 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (313)936-3352 FAX (313)936-3352
jfmjfm-at-engin.umich.edu, John.F.Mansfield-at-umich.edu
or jfmjfm-at-umich.edu they all reach me!
URL: http://www.engin.umich.edu/~jfmjfm/jfmjfm.html






From: JOHNA-at-SCI.WFEB.EDU
Date: Fri, 16 Jun 1995 14:42:47 -0400 (EDT)
Subject: Re: Where is Bio Rad/Polaron?

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Try calling Energy Beam Sciences (800 992-9037; fax 413 789-2786). I
believe that they took over at least part of Bio-Rad/Polaron's line of
ancillary instrumentation.

Good Luck

John

___________________________________________________
| |
| John G. Aghajanian, Ph.D. |
| Worcester Foundation for Experimental Biology |
| 222 Maple Avenue |
| Shrewsbury, MA 01545-2737 |
| |
| Tel: 508 842-8921 ext. 147, 161 |
| Fax: 598 842-9632 |
| JOHNA-at-sci.wfeb.edu |
| |
|_________________________________________________|





From: wise-at-vaxa.cis.uwosh.edu
Date: Fri, 16 Jun 1995 16:04:07 +0000
Subject: EDS of bone medullary tissue

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To all,

We are preparing to use EDS to look for lead in the medullary bone
tissue of geese poisoned by lead shot ingestion. The medullary tissue is
so fragile that we will probably have to embed whole bones in epoxy and
then slice the bone in half. Does anyone have any advice on which epoxy is
preferred? Would polishing of the cut face be necessary? What is used for
a polishing compound? Do the polishing compounds leave contamination?
Etc?
Any help would be appreciated.

Bob Wise
Department of Biology
University of Wisconsin Oshkosh
Oshkosh, WI 54901
(414) 424-3404
(414) 424-1101 fax
wise-at-vaxa.cis.uwosh.edu






From: bozzola-at-siu.edu (John. J. Bozzola)
Date: Fri, 16 Jun 1995 16:39:35 -0600
Subject: SEM:using virus as surface label

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We are revising a textbook and are looking for a SEM micrograph
illustrating the use of viral particles (or possibly haemocyanin) for
labeling cell surfaces. Can anyone help? Thank you.

John J. Bozzola
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
Phone: 618-453-3730
Fax: -2665
Email: bozzola-at-siu.edu OR bozzola-at-qm.c-cem.siu.edu






From: William Tivol :      tivol-at-wadsworth.org
Date: Fri, 16 Jun 1995 16:23:36 -0500 (EDT)
Subject: Re: How hot is Uranyl acetate

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Dear Sally,
My Radiation Sciences class does something like this every year.

1) Look up the half-lives and fractions of uranium isotopes (ignore
14C, 3H and 15O, etc.).
2) Calculate the decay rates from lambda = .693/halflife.
3) Calculate the number of uranium atoms in a unit volume of solu-
tion (this depends, of course, on the strength of the solution).
4) Calculate the number of decays from dN/dt = - lambda N.
5) Sum the contributions from each isotope (if necessary, or if
anal retentive).
6) Convert to Ci/l (or other familiar units).

We treat small amounts of 1% uranyl acetate as low-level waste,
which can be poured down the sink. The short answer, therefore, is
"Not very hot."
Yours,
Bill Tivol




From: m.dickson-at-unsw.edu.au (Melvyn R. Dickson)
Date: Fri, 16 Jun 1995 10:38:44
Subject: Re: Coated grids for Acrylics?

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In article Goldmarker-at-aol.com writes:


} Why is it necessary to use coated grids for acrylic embedding resins, but not
} for epoxy resins?

The electron beam breaks chemical bonds and heats the specimen locally to
around 200 deg celsius. Even epoxy resins lose about half their mass by
evaporation under irradiation before they stabilise as a non volatile
residue. This explains why thicker sections tend to "clear" with the
background becoming more transparent and the metallic stain precipitate
becoming denser (it is becoming physically denser) as the beam hits them.
Acrylic resins are much less stable in the electron beam than epoxy resins
and melt and lose mass to the extent they lodse their mechanical strength and
tear and shrink up to the grid bars.

This ties in with the phenomenon of contamination, in which the vaporised
organic materialsfrom the section redeposit on the section, where they are
depolymerised into less volatile carbon by the beam. This adds mechanical
stability at the cost of some loss in contrast. You can stabilise your
sections this way, but its more controlled to put a very thin carbon coat on
your sections (maybe both sides) in a carbon coater.




From: GVKM07A-at-prodigy.com (DR CHARLES A GARBER)
Date: Thu, 15 Jun 1995 23:42:30 EDT
Subject: TEM, Si thin sectioning

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-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --

On June 15, it was asked "if one can cross-section a 25 micron thick
silicon piece with a diamond knife (microtoming)". And it was also
asked "Will that damage or dull the knife?"

1] One can do it, however it takes a lot of practice, and even then
the quality of the sections is always less than what would be desired
(the section tends to break up because it is so brittle, yet not that
badly that one can not get useful information)

2] It definitely will "damage" or "dull" the knife. You should
collect damaged diamond knives from colleagues and use them for the
rougher part of the operation, e.g. "facing off" the block, for
example. I could tell you horror stories where the user "broke" the
diamond in the knife on his first attempt. Yet others have related the
opposite experience, that is, they were able to cut some number of
samples before the knife was totally kaput.


We have been using our own SPI Materials Science diamond knife for this
kind of sectioning, but I would expect that other "brands" of diamond
knives could be used as well. If you sent us a small piece we could
determine to what degree one could actually do this with your
particular kind of sample in our own "working" laboratory and send you
back a few grids for you to "inspect" in your own TEM. If we could cut
your type of sample satisfactorily, then I would expect that you could
eventually do it as well.

======================================================
Charles A. Garber, Ph. D.
President
SPI SUPPLIES
PO BOX 656
West Chester, PA 19381-0656 USA

Ph: 1-(610)-436-5400
1-(800)-2424-SPI

FAX: 1-(610)-436-5755

e-mail: GVKM07A-at-prodigy.com
SpiSupp-at-aol.com [SPI Customer Service e-mail box]
======================================================







From: A. Kent Christensen :      akc-at-umich.edu
Date: Thu, 15 Jun 1995 15:26:21 -0400 (EDT)
Subject: Re: EM AND LM IMMUNOSTAINING: Ab to FITC

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Jay,

You might want to check with Amersham (technical 1-800-341-7543,
order 1-800-323-9750). They have a non-radioactive ISH system based on
using fluorescein as the marker on the nucleotide, then ICC detection of
the fluorescein with a Fab fragment labeled with alkaline phosphatase
(BCIP/NBT). Your ICC would be equivalent to the detection step in their
system. I don't know about EM (ask them).

A. Kent Christensen, University of Michigan, {akc-at-umich.edu}

--------------------------------

On Thu, 15 Jun 1995, Jay Jerome wrote:

}
} I have a client who wishes to immunostain for FITC. He would like to do
} this at the light microscope level in both parafin and fixed,
} cryosectioned material. He would also like to do this at the EM level by
} pre-embed immunostaining.
}
} My questions are:
}
} 1. Any recommendation on which of the Anti-FITC anti-bodies work well (or
} don't work) for immunhistochemistry? [species the antibody was raised in
} is not limiting at this point]
} a. Parafin embedded material?
} b. Crosections?
} c. Fixed, but unembed material?
}
} 2. Can anyone provide a good reference (I have a couple of papers but the
} methods are sketchy) or protocol for any of the three above staining
} situations with Anti-FITC? Concentrations, times, etc?
}
} 3. Are there any unusual pitfalls to worry about? We have considerable
} experience with immunostaining, so have the basics down.
}
} I appreciate any help y'all can provide. Thanks
}
} Jay Jerome
} **************************************************************
} * aka: W. Gray Jerome *
} * Dept. of Pathology *
} * Bowman Gray School of Medicine of Wake Forest University *
} * Medical Center Blvd *
} * Winston-Salem, NC 27157-1092 *
} * 910-716-4972 *
} * jjerome-at-isnet.is.wfu.edu *
} **************************************************************
}
}




From: dlb-at-aruba.ccit.arizona.edu (David Bentley)
Date: Fri, 16 Jun 1995 17:03:37 -0700
Subject: Re: TEM / Bio / Uranyl Acetate

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The Mallinkrodt MSDS sheet lists the specific activity of UA as
around 0.2 microcuries/gm. Meter readings from our Geiger counter show 300
cpm (.1 mrem/hr) about 2 cm from the side of a 5gm bottle and 8,000 cpm (~3
mrem/hr) 2 cm
from the top of an open bottle. The glass shields the weak x-Ray (0.048
mev) fairly well. A quarter pound bottle had 1,000 cpm (~.4 mrem/hr) at the
sides and 20,000 cpm (~10 mrem/hr) at the open top. Larger quanities of UA
would have to be scaled up appropiately.

In noting another response, you may want to check the local
ordanances, is is possible that the sewer stream in your area is restricted.
Unless my quick calculations are wrong (and they often are) even 1 drop of
saturated UA would exceed our restrictions of 12 picocuries/day. I would
agree that compared to most other isotopes, UA is a low level radiation
source (but it's hazards, especially as a heavy metal, should not be ignored)

Off the topic but for the question of UA not going into solution
asked earlier, one solution is given in Millonig's book Laboratory Manual of
Biological Electron Microscopy, (1976). Adding a drop or two of glacial
acetic acid per 100 mls of water will help the covert the insoluable forms
to soluable UA. It also helps prevent precipitation and increase shelf
life. We have a stock bottle of saturated UA solution in a 1 L brown
bottle, which periodically is added more DI water and more UA. From this is
decanted what is needed and lower % solutions diluted. UA is saturated at
7.7% (15 C.)(Hayat 3rd ed), for room temp, ~8%.




later
dlb





From: GVKM07A-at-prodigy.com (DR CHARLES A GARBER)
Date: Fri, 16 Jun 1995 23:51:36 EDT
Subject: Bio-Rad/Polaron?

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-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --

On June 16, Matt Kizerian asked what happened to "Bio-Rad/Polaron"?

Several years ago, the EM lab "prep" business, which was part of the
Microscience Division of Bio-Rad was sold to Fisons Instruments, and
once over at Fisons, they divided the business into three parts:

a) Histology equipment which was sold to Energy Beam Sciences

b) EM equipment business which was taken over by Fisons VG Microtech,
and their distributor in the USA is Energy Beam Sciences, and

c) Polaron consumables which was taken over by Fisons "corporate". In
Sept. 1994, the entire inventory of the consumables business was
purchased by SPI Supplies, and is being offered to those who want to
continue to use some of the unique and different items from the Bio-Rad
EM consumables catalog not available anywhere else.


You can reach Energy Beam Sciences on (800) 992-9037. They should be
able to help you with what you need. If not, let me know and I will
try to get what you need. In our own laboratory we have an
Oxford/Hexland system and I would imagine the "protocols" are virtually
the same.

For your information, Bio-Rad is very much "still out there" however
the part that we had been all dealing with as part of their "EM lab
prep business" has not been a part of Bio-Rad for some several years.

======================================================
Charles A. Garber
President
SPI SUPPLIES
PO BOX 656
West Chester, PA 19381-0656 USA

Ph: 1-(610)-436-5400
1-(800)-2424-SPI

FAX: 1-(610)-436-5755

e-mail: GVKM07A-at-prodigy.com
SpiSupp-at-aol.com [SPI Customer Service e-mail box]
======================================================







From: Zhiyu Wang :      zhiyu-at-uhunix.uhcc.Hawaii.Edu
Date: Sat, 17 Jun 1995 01:19:53 -1000
Subject: Re: mocha image analysis overlapping globules

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Hi,

This message is for Mr. Diane regarding the usage of MOCHA. I have
experience in measurement of images using MOCHA. It is hard to have
the program to identify the object being overlayed, but you can "help" it.
Go to annotation function, choose free draw, select one of four overlay
color, and draw on the object bandary, then fill out with the overlay color.
Now you can get measurement automaticaly by measuring the overlay object.
You can draw two objects being overlayed by using different color and
signe the colors as two source to be measured.

Good luck!

Wang




From: gt0194g-at-prism.gatech.edu (Joseph E. Coury)
Date: Sat, 17 Jun 1995 10:09:44 -0400
Subject: SPM WWW SITES

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I am currently undertaking the task of compiling a list of World Wide Web
sites dealing with SPM and "Nano" technologies. Is there anyone out there
who has a similar list that might be willing to share it with me? Thank-you
in advance for any help that you can give me.
---------------------------------------------------
Joseph E. Coury
School of Chemistry and Biochemistry
Georgia Institute of Technology
Atlanta, GA 30332-0400 USA

(e-mail) gt0194g-at-prism.gatech.edu
(FAX) 404-894-7452
(LAB) 404-894-4064
---------------------------------------------------





From: M.V. Parthasarathy :      mvp2-at-cornell.edu
Date: Sat, 17 Jun 1995 11:57:16 -0400 (EDT)
Subject: RE: SPM (any type)/plant cell walls

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X-NUPop-Charset: English

In message Fri, 16 Jun 1995 09:17:43 -0500,
baskin-at-biosci.mbp.missouri.edu (Tobias Baskin) writes:

} Greetings,
} Is there anyone out there who is doing (or who knows of someone
} doing) spm on isolated plant cell walls? I am interested in this myself
___________

Please contact Dr. Thomas Pesacreta, Microscopy Center, University of South
Western Louisiana, PO Box 42451, Lafayette, LA 750504-2451 (Tel: 318 231-5769;
Fax: 318 231-5864). I believe he has or he is currently studying plant cell
walls using an SPM.

*************************************************************************
M.V. Parthasarathy
Professor of Plant Biology & Director, EM Facility
Section of Plant Biology
228 Plant Science Building
Cornell University, Ithaca, NY 14850
Telephone: (607) 255-1734
Fax: (607) 255-5407
E-mail: mvp2-at-cornell.edu
***********************************************************************




From: Zhiyu Wang :      zhiyu-at-uhunix.uhcc.Hawaii.Edu
Date: Sat, 17 Jun 1995 20:28:13 -1000
Subject: Re: mocha image analysis overlapping globulesp

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Hi,

I am responding to Diane Montpetit message regarding to the usage of

MOCHA softwaer. I have some experience in setting and using of this

softwaer. It is hard to have the prograsm to identify the objects

overlayed, but you can "help" to do it. Go to ANNOTATION function and

select free draw tool. Draw on the bandary of the object then fill it

with one of four color. Draw another object and fill it with different

color. Now you can have the program to measure the object in different

overlay of color by setting the colors as a source in MEASUREMENT SETTING

window.

Let me know if you have better idea. Good luck!

Wang




From: Dr. Molnar Peter :      MOLNARP-at-lib.dote.hu
Date: Mon, 19 Jun 1995 10:14:16 +100
Subject: Fe identification

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Hi Folks:
Is there anyone out there who knows a specific method that is usable
in conventional TEM for unequivocal identification of iron? I have a
case of chronic granulomatous disease with Prussian blue deposition
in macrophages. I would like to have a similar reaction done in EM.
Any suggestions would be welcome.
Thanks
Peter Molnar M.D., Ph.D.
Hungarian-Japanese EM Ctr
Dept. Pathology, Univ. Med. Sch.
Debrecen, POB. 24.
Nagyerdei krt. 98.
H-4012 Hungary, Europe
FAX/Tel:36-52-417-063
e-mail:molnarp-at-lib.dote.hu




From: randio-at-fagmed.uit.no (Randi Olsen)
Date: Mon, 19 Jun 1995 15:13:49
Subject: EM AND LM IMMUNOSTAINING: ab to FITC

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Last week, Jay Jerome had some questiones about antibodies to FITC.

We have been working with endocytosis of circulating collagen; labelling
collagen with FITC and injection of this collagen into a fish.
For LM detection of the FITC/collagen we just fixed the tissue, embedden in
parafin, sectioned and put it under a fluorescense microscope.
If you want to stain the tissue, make sure you don't use eosin, its a
fluorochrome itself.
For EM studies we used cryosections (fixed with 8% formaldehyd and 0,5 %
glutaradelhyde) Monoclonal Anti FITC from Boehringer Mannheim Biochem,
Germany worked well at a dilution of 1: 100. Bridging antibody not needed.
We used a standard Protein A-gold prosedure.

We also did a adsoption control, and got no signal.

For more details, see Cell and Tissue Research (1995) 280:39-48 ; Smedsrod
et al: Circulating collagen is catabolized by endocytosis mainly by
endothelial cells of endocardium in cod.

Best regards
Randi Olsen
University of Tromso
Department of Electron Microscopy
MH-Breivika
N-9037 TROMSo
Norway





From: TIETZ :      100115.66-at-compuserve.com
Date: 19 Jun 95 09:17:34 EDT
Subject: TEM Cryo Workshop 1995

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All interested microscopists are invited to the CRYO-MICROSCOPY WORKSHOP
from August 28th up to September 1st 1995 organised by the Dutch Society of
Electron Microscopy and Philips Electron Optics

In view of the increasing interest and in order to further cryo-electron
microscopy,
special workshops have been organized since 1989 every two years by Philips
Electron
Optics in cooperation with the Electron Microscopy Society of The Netherlands.
The next workshop will be from August 28th up to September 1st. Experts in the
various fields will teach many aspects of cryo electron microscopy in
theoretical
and perhaps more importantly practical sessions. The workshop will be held at
the
Philips Electron Optics Applications Laboratory with its wide range of essential

equipment, including microscopes, ancillary equipment. Other, specialized
equipment
is made available by several other manufacturers.
A number of cryo-experts is invited as teachers for the workshop.

TOPICS

Cryo-ultra microscopy and Immunogold labelling Freeze substitution.
Cryo-electron microscopy:
- Image Analysis of Spherical Virus.
- Automatic electron tomography of ice-embedded molecules.
- Energy filtering of isolated biological macromolecules.
- 2 Dimensional Protein Crystals in Ice.
- Electron Dosis and the use of a Slow Scan C.C.D.

INVITED SPEAKERS

A. Brisson, T. Baker, B. Koster, H. Gross, W. Voorhout, J. Leunissen, B. Humbel,
G. Knoll, J.W. Slot.

ORGANISERS

Prof. Dr. A. Verkleij, University of Utrecht
Dr. P. Frederik, University of Limburg
W. Busing, Philips Electron Optics

FORMAT

The workshop duration will be 5 days. For the first 4 days, each activity will
include two one hour sessions as an introduction for the practical sessions.
The practical sessions will be organised by cryo-specialists from the
Universities of Utrecht and Limburg and participants will have the opportunity
for hands-on-practise and to work with their own specimen.

The fifth day, the morning will also start with theory sessions and after this,
the cryo-course will be evaluated. In the afternoon the program is open to give
the participants time for travel arrangements and/or to continue to work with
their own specimen.

Registration form available from (the dead line is July 1,1995):

W.M. Busing
Philips Electron Optics
Building AAE
P.O. Box 218
5600 MD Eindhoven
The Netherlands
Fax: +31 40 766102 or

Tietz Video & Image Processing GmbH
Herbststr. 7
D-82131 Gauting
Phone: 089/8506567
Fax: 089/8509488

e-mail: 100115.66-at-Compuserve.COM






From: A. Kent Christensen :      akc-at-umich.edu
Date: Mon, 19 Jun 1995 09:56:34 -0400 (EDT)
Subject: Re: Using X-gal for ?-galactosidase demonstration at EM level

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Terry,

I have heard that time in propylene oxide needs to be reduced. Some
references that include EM of X-gal product:
Engelhardt et al., 1991, PNAS 88:11192.
Franklin & Barnett 1991, Acta Neuropath 81:686.
Loewy et al. 1991, Brain Res 555:346.

A. Kent Christensen, University of Michigan, {akc-at-umich.edu}

-------------------------------------------

On Fri, 16 Jun 1995, T. Robertson wrote:

}
} To any myologists out in cyber space
} I am attempting to demonstrate ?-galactosidase in a strain of lacZ mice
} (transgenic mice strain for fast troponin) using X-gal histochemistry and
} conventional processing for transmission electron microscopy. Although I can
} clearly see labelled nuclei at the light microscope level I am having
} trouble routinely seeing positive cells at the TEM level. Does anyone have
} experience in this field and is there a step in the processing that might
} remove much of the reaction product. We dehydrate in graded ethanol, use
} propylene oxide as a transitional solvent and embedd in Araldite. I would
} be grateful for any helpful comments.
}
} Terry
}
} Dr Terry Robertson
} Electron Microscopist
} Department of Pathology
} University of Western Australia
} Nedlands 6009
}
} phone 346 2935
} Fax 346 2891
} email troberts-at-eosin.path.uwa.edu.au
}




From: cook-at-aaem.amc.anl.gov (Russell E. Cook)
Date: Mon, 19 Jun 1995 11:10:03 -0600
Subject: electropolishing cast iron

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Message-Id: {v01510101ac0b5e65bbc8-at-[146.139.72.78]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

1. See Ultramicdoscopy 25 (1988) 89-90. It describes 65 ml HCl, 435 ml
methanol, and 20 ml butyl cellosolve (a viscosity improver) at -20 C, 110
volts, 120 mA on a single jet South Bay instrument.

2. Also try: 50 ml perchloric acid, 450 ml acetic acid, and 50 ml butyl
cellosolve at room temp., 42 volts, 24 mA. Again, this was on a South Bay
550-B single jet instrument. Great results on cast stainless steel,
furnace aged.

Who has a good D.C. electropolish for platinum ??? A.C. in the literature!!


}
} *From: "A. HONARBAKHSH-RAOUF" {CER5AH-at-ECU-01.NOVELL.LEEDS.AC.UK}
} *To: microscopy-at-aaem.amc.anl.gov
} *Subject: cast iron thin foil
}
} *Does anyone know any suitable solution for preparation of thin
} *foil using jet polishing of ductile cast iron(presence of two
} *different phases ie free graphite and matrix)? Is there any other
} *method except ion beam?
} *Thanks
} *Abbas

Russell E. Cook
Electron Microscopy Center for Materials Research
Argonne National Laboratory
9700 South Cass Avenue
MSD-212
Argonne, IL 60439
(708)252-7194
FAX: (708)252-4798






From: John.Wheatley-at-ASU.Edu (John C. Wheatley)
Date: Mon, 19 Jun 1995 09:40:59 +0800
Subject: Legal/Ethical Question

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X-Sender: wheatley-at-csss.la.asu.edu
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I am interested in the legal and ethical arguments (pro and con) for
university involvement with industrial entities in the area of electron
microscopy. In other words, is it legal/ethical for universities to use
university instruments to solve problems for industry on a fee-for-service
basis? I would like to have replies from anyone who wishes to respond.
However, I realize that regulations may be different in other countries and
therefore I ask that your responses be sent directly to me so our
international friends are not bored with American legal/ethical
discussions. I will provide a compilation of the responses to all who ask
for them, except when individual respondents request otherwise.

With respect to the legal side of the question, I would like to have
answers based upon actual legal opinions that you are aware of.
Perceptions about legality will not be useful. I know that there are
probably very few (maybe none) lawyers involved in electron microscopy. If
your institution has issued a legal opinion with respect to this issue, I
would like to know what that opinion is.

I would find it useful to have many people respond to this request. I
would especially like to have those members of the private sector, who use
electron microscopes to solve problems for industry, respond.

If you feel comfortable responding, please include your price structure for
industrial jobs--proprietary and otherwise.

I thank you for your responses.

John C. Wheatley
Lab Manager
Center for High Resolution Electron Microscopy--Arizona State University

John C. Wheatley
Lab Manager
Center for High Resolution Electron Microscopy
BOX 871704
Tempe, AZ 85287-1704
Phone: (602) 965-3831
FAX: (602) 965-9004
John.Wheatley-at-ASU.Edu






From: Jaime Dant :      jaime-at-borcim.wustl.edu
Date: Mon, 19 Jun 1995 12:55:58 -0500
Subject: re: how hot is ua

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Good afternoon Sally,

I purchase UA from EM Sciences and they print right on the bottle its activity
as 0.51 uCi/gm.

Jaime A. Dant




From: wzp-at-befvax.uchicago.edu
Date: Mon, 19 Jun 1995 12:59:19 EDT
Subject: subscribe

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Zhiping Wang from the University of Chicago





From: modb-at-ruca.ua.ac.be (Marc Op de Beeck)
Date: Mon, 19 Jun 1995 21:00:40 +0200
Subject: HREM SIMULATIONS COMPARISON

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Dear HREM collegues,

Together with Dr. Dirk Van Dyck, I started a comparison of different
HREM diffraction simulation programs in order to check consistency.
After some "false" starts (personal mailings, request in Ultra-
microscopy, ...) on which we got hardly no reaction at all, we finally
decided to start the comparison with the programs we had available at
the Universities of Antwerp (Belgium) and Amsterdam (The Netherlands).

Together with collegues in Amsterdam/Delft University, we tested some
commercially available programs we had access to, such as :

* MacTempas : done
* Cerius2 : done
* EMS Multislice : in progress
* EMS Bloch Wave : in progress

against some non-commercial ones, as :

* Real Space : done Dirk Van Dyck, Wim Coene, ...
* ONERA, Bloch wave : done Cyrille Barreteau
* ONERA, Multislice : done Cyrille Barreteau
* TCBED : done Zuo and Spence
* Dong, Multislice : done Dong Tang
* Chen, Multislice : in progress Jianghua Chen
* NCEMSS Multislice : in progress Roar Kilaas

With these results, I created a WWW site "http://www.ruca.ua.ac.be/~modb"
which has been made known to the commercial authors of the following
packages :

* Kilaas : MacTempas, NCEMSS
* O'Keefe : SHRLI
* Molecular Simulations : CERIUS2
* Stadelmann : EMS
* Epicier : SIMPLY (shareware)

Seeing these results, all authors finally have agreed to contribute to
the project (and if necessary, change their programs according to the
requirements of the proposal such as the use of different potentials).
Up till now, the comparison with MacTempas and the CERIUS2-HRTEM packages
have been finished in collaboration with the respective authors. EMS will
be included shortly (in he next two weeks) in the overview. The present
results show that all packages can reproduce the same results, provided
they are properly used (which quite often means that using the standard
values in these programs is NOT appropriate to get fully converged
results).

It is however clear that not all currecntly used packages have been
included in this survey. We simply choose for the packages above, since
they were available to us. Therefore, all authors and users of these
"neglected" packages are kindly requested to contribute to this
comparison. The full text of the proposal can be found in
* "Ultramicroscopy 55 (1994), 435-437"
* WWW site "http://www.ruca.ua.ac.be/~modb"


Sincerely yours,

Dr. Marc Op de Beeck
University of Antwerp (RUCA-EMAT)
Groenenborgerlaan 171
B-2020 Antwerpen
BELGIUM

Tel : ++ 32 3 218 02 61
Fax : ++ 32 3 218 02 57
URL : http://www.ruca.ua.ac.be/~modb
E-mail : modb-at-ruca.ua.ac.be






From: c4647-at-slvaxa.umsl.edu (Phil Fraundorf)
Date: Mon, 19 Jun 1995 14:15:28 -0500
Subject: Re: Legal/Ethical Question

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Yo John,

You wrote for the ANL microscopy listserver:
} I am interested in the legal and ethical arguments (pro and con) for
} university involvement with industrial entities in the area of electron
} microscopy. In other words, is it legal/ethical for universities to use
} university instruments to solve problems for industry on a fee-for-service
} basis? I would like to have replies from anyone who wishes to respond.
} However, I realize that regulations may be different in other countries and
} therefore I ask that your responses be sent directly to me so our
} international friends are not bored with American legal/ethical
} discussions. I will provide a compilation of the responses to all who ask
} for them, except when individual respondents request otherwise.

It's NICE that you should ask about ethics, even if legal had a tendency
to come first. I have some experience with mandates on "both sides of the
aisle", and have in fact recently discovered that development of a "Code of
Ethics for Analytical Support" can have important PRACTICAL consequences for
the kind of collaborations you describe, as well as for the development of
constructive legal precedents in days ahead. Look for benefits to
university, industry, and even instrument researchers, as well as to the
appropriate pooling of resources between institutions.

Using as template codes developed in other fields (e.g. occupational
therapy, for example), we are in the process of preparing an article with
some specifics. One objective of this initiative is to provide a mechanism
which will allow us to avoid asking questions of lawyers BEFORE they are
equipped to answer them (something that generally has a negative impact on
everyone involved). In that context I expect that what you will find out
there now, in your survey, may not be terribly enlightened. Nonetheless, I
am interested, and will try to keep you posted on our initiatives in this
vein as well.

One question I have of LISTSERVER MEMBERS in this context is: What work
on a code of ethics for researchers in analytical support (like microscopy)
is already available to draw from? (As with John's request, feel free to
reply directly rather than through the listserver.)

Cheers. /philf :)

//\/\/\/\--}
// Phil Fraundorf, Physics & Astronomy: 3145165044 c4647-at-slvaxa.umsl.edu
\\ U. Missouri - St. Louis MO 63121 USA http://newton.umsl.edu/pfhomepg
\\/\/\/\/\/\/\/--}





From: wise-at-vaxa.cis.uwosh.edu
Date: Mon, 19 Jun 1995 15:37:17 +0000
Subject: Legal/ethical question

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In response to John.Wheatley's Legal/Ethical Question:

The legislature of the State of Wisconsin has addressed this issue
and has written statutes that very clearly delineate what university
facilities can and cannot be used for and under what terms. Basically,
non-university users can rent beam time but they must be charged a
competitive rate so as not to undercut private testing labs. I have
responded in much more detail directly to John.

Another side of the issue, however, is the direct impact on the
laboratory director. I am an assistant (tenure-track) professor at the
Uniersity of Wisconsin Oshkosh. I am allowed to do as much consulting as I
wish and charge whatever rate I want to as long as it does not interfere
with my job duties as described in my contract and the Faculty Handbook.
The Handbook says that I will be evaluated (for retention, promotion,
tenure, merit pay increases, etc) 45% on my teaching, 45% on research and
10% on service to the governance of UWO. Performing research for private
industry is not included anywhere in my job evaluation so even though I may
make a zillion dollars an hour, I am doing nothing to gain tenure. I
suppose that if I were Professor Super Microscopist I could handle all of
my teaching duties, publish 10 times a year, head the Faculty Senate, and
pull in $200,000 a year in outside consulting. But I'm not.

In short, although my dean has not forbidden me from taking on
outside work, he has made it very clear that I will get no credit for it
when it comes tenure time. I have ocassionally done this sort of work for
people or institutions (most of it gratis) that really needed my help but,
on the whole, I avoid it if I can.

Bob Wise
Director, UWO EM Facility
Univresity of Wisconsin Oshkosh
Oshkosh, WI 54901
(414) 424-3404
(414) 424-1101 fax
wise-at-vaxa.cis.uwosh.edu






From: mager-at-unixg.ubc.ca (Mary Mager)
Date: Mon, 19 Jun 1995 23:26:08 -0800
Subject: legal/ethical question

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X-Sender: mager-at-pop.unixg.ubc.ca (Unverified)
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Mime-Version: 1.0
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Dear John,
I run a multi-user Materials EM lab at the U. of British Columbia and this
question has come up in the past, particularly when the first commercial
Failure Analysis consultant bought his own SEM and hired my predecessor
away. The deal worked out with this consultant and consolidated since is:
1. The undergrad labs have first priority in the university lab, the
graduate student work the next. The other university users, who pay an
hourly fee ($25/hr) far less than the commercial rate, have next priority.
2. Commercial work, when done in time extra to that needed for university
work, is charged at a rate exceeding that of the local commercial
suppliers. It is important that there is no unfair competition with
government-funded equipment.
There were no legal precedents that I could find at the time this
arrangement was set up (1981). The consulting company sometimes finds it is
necessary to have another lab perform tests on a sample if they represent
one side of a litigation, so our relationship with the "competing" lab is
friendly. I send work to them if it is more their thing, they send them to
me if it is something I can do better.
The service I provide is strictly beam time, photos, EDX analysis and a
brief note on what I found. I am a technician, not a professor, so this is
not a consulting job, rather a lab service.
I have heard the opinion from some Americans that this kind of arrangement
is considered criminal conflict of interest, but here there doesn't seem to
be a problem. You were not very clear on the exact nature of the
relationship you wish to establish with the industrial entities, but I
cannot see the benefit in forbidding it altogether. The money coming in,
since it is at a high commercial rate ($200/hr.), is very welcome to help
run the lab.
I hope this overly-long reply is some help.

Mary Mager
Electron Microscopist
Metals and Materials Eng, UBC
309 - 6350 Stores Rd.
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648, fax: 604-822-3619
email: mager-at-unixg.ubc.ca






From: Mike Witcomb :      MIKEW-at-gecko.biol.wits.ac.za
Date: Tue, 20 Jun 1995 15:29:06 GMT+2
Subject: electropolishing platinum

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In answer to Russel Cook's question on the above, I have found the
best solution is that of Tousek (Praktische metall. 7, 202, 1970)
which is:
equal volumes of nitric, sulphuric and phosphoric acids at about 3
volts ac.
I use a platinum strip as the other electrode. I normally pre-age the
solution for about two hours using two platinum electrodes at 3-3.5
volts ac. The solution turns from colourless to yellow. I keep the
solution temperature at 20 centigrade for pre-aging and polishing.
I have obtained conventional, HREM and ARM images using this method.


Dr MJ Witcomb
Electron Microscope Unit
University of the Witwatersrand
Private Bag 3
WITS
2050
South Africa

Telephone: + 27 11 716 4000
+ 27 11 716 2419 (messages)
Fax: + 27 11 339 3407
E-mail: mikew-at-gecko.biol.wits.ac.za




From: M.V. Parthasarathy :      mvp2-at-cornell.edu
Date: Tue, 20 Jun 1995 11:23:48 -0400 (EDT)
Subject: Job Opening/Technician

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X-NUPop-Charset: English

EM TECHNICIAN

A technician's position is available from July 3rd at The Electron Microscopy
Facility, College of Agriculture and Life Sciences, Cornell University for
teaching the laboratory portions of SEM and Freeze-fracture courses. The
applicant would be expected to spend a major part of the time training,
instructing, assisting and supervising students, postdocs and faculty on the
proper use of the equipment in the facility. Rest of the time would be
devoted to research service, maintaining equipment and up keep of the
laboratories. Applicants should have at least a B.S. degree in science,
preferably in biology. At least three years of experience in SEM, basic skills
in instrumentation and familiarity with computers is essential.
Familiarity with TEM techniques and Freeze-fracture is desirable but not a
requirement. The facility is well equipped and includes a Hitachi 4500 FESEM
and two Bal-Tec Freeze-fracture units. The applicant would work with
another senior technician in the Facility who would be the applicant's
immediate supervisor. Salary range is from $19,350 to $26,600.

Inquiries and applications should be directed to Dr. M.V. Parthasarathy at
the address indicated.

*************************************************************************
M.V. Parthasarathy
Professor of Plant Biology & Director, EM Facility
Section of Plant Biology
228 Plant Science Building
Cornell University, Ithaca, NY 14850
Telephone: (607) 255-1734
Fax: (607) 255-5407
E-mail: mvp2-at-cornell.edu
***********************************************************************




From: Jay Jerome :      jjerome-at-isnet.is.wfu.edu
Date: Tue, 20 Jun 1995 13:02:00 -0400 (EDT)
Subject: Re: Legal/Ethical Question

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We routinely do work for local industry. Our University Legal Counsel
advised that as long as we did not use university resources to undercut
local competition we were O.K. It is also not a good idea to upset local
industry by undercutting their prices, because you may need their help
some day.
An example of the legal issues:

One of our microscope's service contract is payed for from NIH grant
support. It would be against our grant agreement and the law to charge
industry the same low rate we provide to academic users, because academic
users do not pay the full cost of instrument time (NIH grant picks up
those costs involved with service contract). Our rate to industrial users
MUST include compensation for part of the service contract. We also get
lights and electricity from the University. Our industrial rate must
include a component to compensate for these expenses.
Bottom line: you must make sure your rate reflects a level playing field
with other non-academic suppliers of similar services.

I hope this helps:

Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-isnet.is.wfu.edu *
**************************************************************

On Mon, 19 Jun 1995, John C. Wheatley wrote:

} I am interested in the legal and ethical arguments (pro and con) for
} university involvement with industrial entities in the area of electron
} microscopy. In other words, is it legal/ethical for universities to use
} university instruments to solve problems for industry on a fee-for-service
} basis? I would like to have replies from anyone who wishes to respond.
} However, I realize that regulations may be different in other countries and
} therefore I ask that your responses be sent directly to me so our
} international friends are not bored with American legal/ethical
} discussions. I will provide a compilation of the responses to all who ask
} for them, except when individual respondents request otherwise.
}
} With respect to the legal side of the question, I would like to have
} answers based upon actual legal opinions that you are aware of.
} Perceptions about legality will not be useful. I know that there are
} probably very few (maybe none) lawyers involved in electron microscopy. If
} your institution has issued a legal opinion with respect to this issue, I
} would like to know what that opinion is.
}
} I would find it useful to have many people respond to this request. I
} would especially like to have those members of the private sector, who use
} electron microscopes to solve problems for industry, respond.
}
} If you feel comfortable responding, please include your price structure for
} industrial jobs--proprietary and otherwise.
}
} I thank you for your responses.
}
} John C. Wheatley
} Lab Manager
} Center for High Resolution Electron Microscopy--Arizona State University
}
} John C. Wheatley
} Lab Manager
} Center for High Resolution Electron Microscopy
} BOX 871704
} Tempe, AZ 85287-1704
} Phone: (602) 965-3831
} FAX: (602) 965-9004
} John.Wheatley-at-ASU.Edu
}
}
}




From: Jay Jerome :      jjerome-at-isnet.is.wfu.edu
Date: Tue, 20 Jun 1995 13:10:28 -0400 (EDT)
Subject: Re: Legal/ethical question

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Bob Wise makes some good points about supporting local industrial
research not contributing to tenure decisions. However, one benefit that
can acrue from doing consulting EM work for outside industry is that the
monies generated often can be put into a discretionary fund you have
control over. This is a good way pay for lab equipment that Deans and
Government grants won't.


Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-isnet.is.wfu.edu *
**************************************************************





From: Michael Rock :      merock-at-u.washington.edu
Date: Tue, 20 Jun 1995 10:12:31 -0700 (PDT)
Subject: Re: Fe identification

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Peter-
has anyone suggested using energy dispersive X-ray Spectrometery (EDS)
most analytical EM's are equipped with them. barring any Fe introduced by
sample prep or your grid (use Ni or Au grids) you should identify the Fe
in your sample. Depending on the granules and the resolution of your EDS
system you may be able to resolve whether the Fe is in the inclusions of
throughout the cytoplasm.
-Mike


On Mon, 19 Jun 1995, Dr. Molnar
Peter wrote:

} Hi Folks:
} Is there anyone out there who knows a specific method that is usable
} in conventional TEM for unequivocal identification of iron? I have a
} case of chronic granulomatous disease with Prussian blue deposition
} in macrophages. I would like to have a similar reaction done in EM.
} Any suggestions would be welcome.
} Thanks
} Peter Molnar M.D., Ph.D.
} Hungarian-Japanese EM Ctr
} Dept. Pathology, Univ. Med. Sch.
} Debrecen, POB. 24.
} Nagyerdei krt. 98.
} H-4012 Hungary, Europe
} FAX/Tel:36-52-417-063
} e-mail:molnarp-at-lib.dote.hu
}




From: Michael Cammer :      cammer-at-aecom.yu.edu
Date: Tue, 20 Jun 1995 13:38:21 -0400 (EDT)
Subject: Re: Legal/ethical question

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But if you have support (e.g. from your institution), beware. I know of a
library that had it's institutional $$ support reduced when contributions
(e.g. from alumni) made specifically for the library were increased. In
effect, the supposed additional income made no difference to the library's
ability to operate for that year.
-mc

} Bob Wise makes some good points about supporting local industrial
} research not contributing to tenure decisions. However, one benefit that
} can acrue from doing consulting EM work for outside industry is that the
} monies generated often can be put into a discretionary fund you have
} control over. This is a good way pay for lab equipment that Deans and
} Government grants won't.
}
} Jay Jerome







From: Michael Rock :      merock-at-u.washington.edu
Date: Tue, 20 Jun 1995 10:54:21 -0700 (PDT)
Subject: Re: Legal/Ethical Question

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John-
in response to legal/ethical.
I think they are separate issues and need to be tackled as such.
1) legal
I have been involved in such a system at a previous place of employment. We
operated as a "revenue generating" entity within the university, and set
up a system where we could do "service work" for outside clients. By doing
this we also had to charge our own users. We set inside rates to a
reasonable level so as not to discourage research, and the industrial
rates equal to or higher than other service labs in the area were
charging so as not to compete with them.
*we stayed legal*
2) ethical
this topic was much harder to define as to yes/no. there is a lot of gray
area out there. I was managing a electron microscopy cost center "revenue
generating facility" within the university, at a previous place of
employment. I agree with the philosophical argument, "to combine the
knowledge and resources of academia with the resources $ available in
industry, to solve practical problems and develop new technologies". BUT
there there are some very tough proprietary questions which need to be
dealt with in advance. Reasonable rates need to be established for faculty
and grad students so as not to discourage use, and higher rates need to
be established for the industrial clients, so there are no charges of
unfair competition from service labs in the area. Then there must be some
way of enforcing such policies on "tenured faculty" who may get lost in
this grey area "fog $$$".
Faculty sometimes had the idea that consulting jobs could be done at the
lower rate, or charged to their grants! This was very hard to monitor
and/or enforce, it relies on personal integrity of all those who are using
and administrating the facility.
good luck
-Mike




From: jandt-at-msc.cornell.edu
Date: Tue, 20 Jun 1995 15:52:43 -0400 (EDT)
Subject: to be forwarded (fwd)

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Reply-To: jandt-at-msc.cornell.edu

Hi Folks,
}
}
} I would like to etch a PET (polyethylterephtalate) film in order to
} see the crystalline part protubating on the surface. My film is 180 microns
} thick, biaxially stretched. The crystallinity is approximatly 40%.
} I would prefer to etch it smoothly, to remove every layer one by one
} (i.e. without any crazing effect if possible). Does anyone know how to do that?
} Thanks a lot in advance.
}
}
jandt-at-msc.cornell.edu





From: baskin-at-biosci.mbp.missouri.edu (Tobias Baskin)
Date: Tue, 20 Jun 1995 15:28:16 -0500
Subject: AFM/DNA on carbon wafer

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Greetings,
A colleague of mine, Jim Sheldon, would like to adhere DNA onto a
carbon wafer for imaging with AFM. Anyone out there know of a relyable
protocol? Apparently, just adding the DNA to the wafer results in clumping
of the DNA. Thus, the carbon surface needs to be activated somehow.
Suggestions?
Thanks in advance,

Tobias Baskin

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
___ ____ ^ ____ _____ Tobias I. Baskin
/ \ / / \ / \ / University of Missouri
/ | / / \ / / Biological Sciences
/___ / /__ /_____\ / /__ 109 Tucker Hall
/ / / \ ( / Columbia, MO 65211 USA
/ / / \ \ / voice: 314-882-0173
/ /____ / \ \____/ /_____ fax: 314-882-0123






From: Glen Macdonald :      glenmac-at-u.washington.edu
Date: Tue, 20 Jun 1995 13:57:37 -0700 (PDT)
Subject: LM:Used Zeiss WL wanted

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X-Sender: glenmac-at-homer01.u.washington.edu

Hello all,
We are looking to buy a used Zeiss WL. With external lamp, stage, and
objective turret. Eyepieces, objectives, camera tube and condenser are
optional. This will be used for brightfield microscopy and photomicrography.

TIA for any offers. Email me directly.


Glen MacDonald
Hearing Development Laboratories
University of Washington
Seattle, WA 98195-6515
(206)543-8360
glenmac-at-u.washington.edu







From: Glen Macdonald :      glenmac-at-u.washington.edu
Date: Tue, 20 Jun 1995 14:41:00 -0700 (PDT)
Subject: TEM:digital imaging

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X-Sender: glenmac-at-homer01.u.washington.edu


Does anyone have some recommendations or warnings about digital image
capture from an TEM? I've been asked to help spec. out a system to be used
on a Philips EM 410.
I think the side mounted detectors with metal bellows have
chronic problems developing vacuum leaks.
What are experiences with 8-bit image depth versus 10-bit or
greater? Tentatively, I'm considering a Mac PowerPC 7100 with a Scion
AG-5 8-bit frame capture board, running NIH Image. Plus the usual
ethernet and optical drive.

Please email directly to cut the bandwidth on this group.

TIA
capture from TEM? I've been asked to help spec. out a system to go onto
a Philips EM410. Side mounted cameras seem to have chronic problems with
vacuum leaks developing in their metal bellows.
Any comments about 8-bit versus 10-bit or greater signal depth as
far as performance relative to cost? Tentatively, we are looking at a Mac
PowerPC (overkill, but fun) with an 8-bit framegrabber board and running
NIH-Image. And athe usual Ethernet and mass storage accessories.

TIA. Please send any commments directly to me.

Glen MacDonald
Hearing Development Laboratories
University of Washington
Seattle, WA 98195-6515
(206)543-8360
glenmac-at-u.washington.edu






From: GVKM07A-at-prodigy.com (DR CHARLES A GARBER)
Date: Tue, 20 Jun 1995 21:55:26 EDT
Subject: AFM/DNA on carbon wafer

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-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --

On June 20, Tobias Baskin mentioned "carbon wafers" as substrates for
AFM.

People seem to be using HOPG (highly ordered pyrolytic graphite) for
this purpose. It comes in several different grades, and there is
considerable variation in price between them.

If you want further information about the HOPG and the different grades
for this application, then we will be happy to send you our product
sheet on the product (which we sell, of course!) just for the asking.
Be sure to send a FAX number for fastest response. For fastest
response, use {SpiSupp-at-aol.com} .

The most expensive grade is for instrument calibration. To use this
"premier" grade for normal use, for most researchers, this would be
overkill. The grade STM-1 is quite acceptable for almost any high
quality research work and our grade STM-2, which we call "student"
quality is apparently quite acceptable for many people as well, but is
is much cheaper. The HOPG is somewhat like mica in that thin layers
can be "stripped" off. So one can get quite a few "strippings" from a
single block of the product.

Chuck

======================================================
Charles A. Garber, Ph. D.
President
SPI SUPPLIES
PO BOX 656
West Chester, PA 19381-0656 USA

Ph: 1-(610)-436-5400
1-(800)-2424-SPI

FAX: 1-(610)-436-5755

e-mail: GVKM07A-at-prodigy.com
SpiSupp-at-aol.com [SPI Customer Service e-mail box]
======================================================





From: Mnr HJ Els :      HJELS-at-op1.up.ac.za
Date: Wed, 21 Jun 1995 11:39:35 GMT+2
Subject: immuno gold labelling

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Which is the best detergent to use in electronmicroscopical immunogold
laballing of intraerythrocytic parasites? ?time; ?concentration?




From: Mnr HJ Els :      HJELS-at-op1.up.ac.za
Date: Wed, 21 Jun 1995 12:28:48 GMT+2
Subject: immuno gold labelling

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Hi,
we are trying to improve immuno labelling.
which is the best detergent to use in electron microscopical
immunogold labelling of intra-erythrocytic parasites (babesia)?
what concentrations can be used?
how long should the detergent be applied (after fixation)?
any suggestions would be greatly appreciated.

E-mail: hjels-at-op1.up.ac.za




From: Richard E. Edelmann :      EDELMARE-at-CASMAIL.MUOHIO.EDU
Date: Wed, 21 Jun 1995 08:04:29 -500
Subject: Re: AFM/DNA on carbon wafer

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microscopy-at-aaem.amc.anl.gov

Tobias,
As Chuck mentioned you will need to use HOPG as the carbon
substraight. I haven't tried DNA yet, but I would suggest using the
Kleinschmidt DNA spreading technique to minimize clumping (sorry I
can't seem to find a reference for the technique presently but it is
very common for TEM preps). Basically the DNA is prepared in a
volatile buffer of ammonium acetate, and mixed with a protein
(cytochrome c), and the mixture is gently applied to to a clean water
surface. The denatured cytochrome c forms a film at the air/water
interface to which whole lengths of DNA as attached. This film could
then be picked up with a freshly cleaved sheet of HOPG (as apposed to
TEM grids, or mica).

Another method I remember comming across which in my mind held
great promise was floating the DNA on a drop of Mercury. As the
mercury is reduced in volume the organic molecules accumulate at the
apex of the drop. Then observation can be made with STM (conductive
mercury). I origianlly saw this technique discribed about 2 years
ago.


Richard E. Edelmann
Electron Microscopy Facility Supervisor
352 Biological Science Building
Miami University, Oxford, OH 45056
Ph: 513-529-5712
E-mail: edelmare-at-muohio.edu




From: Diane Montpetit :      MontpetitD-at-EM.AGR.CA
Date: Wed, 21 Jun 1995 07:54:02 -0400
Subject: mocha image analysis many thanks

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Message-Id: {sfe7da6d.009-at-EM.AGR.CA}
X-Mailer: Novell GroupWise 4.1

many thanks from those who answered my question, I really appreciate the support.

I will certainly try all of the suggestions.

This microscopy group is a blessing ...Thanks Nestor.





From: ubira-at-iqm.unicamp.br (Ubirajara Pereira Rodrigues Filho)
Date: Wed, 21 Jun 1995 09:40:51 -0300
Subject:

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Hi,

Can someone help to obtain the e-mail of SPI supplies or Dr Charles
Garber ?
Thanks in advance,

Ubirajara Pereira Rodrigues Filho

e-mail: ubira-at-iqm.unicamp.br





From: EMLAB-at-opus.mco.edu
Date: Wed, 21 Jun 1995 09:21:52 -0500 (EST)
Subject: Re: immuno gold labelling

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From: ldm2-at-apollo.numis.nwu.edu (L.D.Marks)
Date: Wed, 21 Jun 1995 08:42:05 -0500
Subject: WWW Icons for microscopy

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I would like to announce a "competition" for microscopy related icons
that can be used on the Web. (I am afraid that the only prize will be the
adoption of particular icons by people who develop home pages.) I can
accept icons using anonymous ftp to risc1.numis.nwu.edu - please place
any icons in the directory contrib. I will also accept icons if they
are emailed directly to me as ASCII text. Please note: all icons must
be in standard ASCII or UNIX form, not encrypted Mac language.
As contributions come in I will place them in a directory which
can be accessed via the Web to our homepage at
http://risc1.numis.nwu.edu/internet/lab.html .




From: ldm2-at-apollo.numis.nwu.edu (L.D.Marks)
Date: Wed, 21 Jun 1995 08:49:45 -0500
Subject: HREM Shareware

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Within our homepage (http://risc1.numis.nwu.edu/internet/lab.html) I
am going to start collecting shareware for HREM. The primary categories that
I am thinking of are:
a) Semper (5 or 6) library programs or run programs.
b) X-windows based routines such as simple CTF graphers.
c) Other X-windows type programs.

I can take PC based routines, with many reservations Mac-based programs
but since we are primarily unix/workstation focussed this will be the exception.

Contributions can be sent via anonymous ftp to the directory contrib
at risc1.numis.nwu.edu; any requests for further information or clarification
can be emailed to me.




From: jandt-at-msc.cornell.edu
Date: Wed, 21 Jun 1995 09:52:54 -0400 (EDT)
Subject: PET etching

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Reply-To: jandt-at-msc.cornell.edu


} Hi Folks,
} }
} }
} } I would like to etch a PET (polyethylterephtalate) film in order to
} } see the crystalline part protubating on the surface. My film is 180 microns
} } thick, biaxially stretched. The crystallinity is approximatly 40%.
} } I would prefer to etch it smoothly, to remove every layer one by one
} } (i.e. without any crazing effect if possible). Does anyone know how to do that?
} } Thanks a lot in advance.
} }
} }
} jandt-at-msc.cornell.edu
}





From: hukee.margaret-at-mayo.EDU (Marge Hukee)
Date: Wed, 21 Jun 1995 10:23:24 +0200
Subject: detergent for immunogold

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Message-Id: {9506211521.AA18901-at-fermat.Mayo.EDU}
X-Sender: hukem-at-fermat.mayo.edu
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

In our facility, Tween-20 has been used extensively in both PBS and TBS in
concentrations of .1% for post-embeddeding labeling procedures . But it
sounds as if you are using a pre-embedding technique. Maybe more info is
needed
Marge

Marge Hukee
EM Core Facility hukee.margaret-at-mayo.edu
Mayo Foundation (507) 284-3148
----------------------------------------------------------------------------
--






From: X.m. Burany :      burany-at-sfu.ca
Date: Wed, 21 Jun 1995 10:57:14 -0700 (PDT)
Subject: copy of paper

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Can anyone out there can send me copies of the following paper by Fax.

Schlesinger, M.; Meng, Xianying; Snyder, D.D., J.Electrochem. Soc. 1990,
137(6) 1858-9.

Schlesinger, M.; Meng, X. Evans, W.T.; Saunders,D.A. Kampert, W.P., J.
Electrochem. Soc. 1990, 137(6) 1706-9.

Schlesinger, M.; Meng, Xianying; Snyder, D.D., J. Electrochem. Soc. 1991,
138(2) 406-10.

My Fax No. is (604) 291-3592.

I greatly appreciate your help.

Sandy Burany, PhD


Dept. of Physics
Simon Fraser University
Burnaby, B.C. V5A 1S6
Canada
burany-at-sfu.ca




From: chandler-at-lamar.ColoState.EDU (John Chandler)
Date: Wed, 21 Jun 1995 13:51:19 -0700
Subject: Re: Digital TEM Imaging

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} Glen MacDonald asked about Digital Imaging on a TEM. I hope he realizes that
} the system he is looking at will be useless for producing quality images, and
} will have as its only advantage speed. Even an expensive CCD system (
}
}
}
}
}
} 1024 x
} 1024 x 14 bit) cannot equal film for image quaility, and a TV capture board
} is far below that in quality. Glen needs to carefully define his expectations
} before he goes about specifying a system.
} John Mardinly
} Intel Materials Technology

I would second John's comments. I have been doing some initial
evaluations, and the 1K X 1K images just don't seem to have enough
information to be useful for publication. I'm still waiting to hear from
the folks who were going to show me some images from a 2K X 2K camera.
Perhaps we will see some new capabilities at the MSA meeting in Kansas City
this August.

John
chandler-at-lamar.ColoState.EDU
http://www.vetmed.colostate.edu/anatomy/faculty/chandler.html






From: sulovsky-at-sci.muni.cz (Petr Sulovsky)
Date: Thu, 21 Apr 1994 17:53:50 -0400
Subject: PE tubing for INTEGREX Colourjet Printer

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Message-Id: {199506211556.AA09849-at-elanor.sci.muni.cz}
X-Sender: sulovsky-at-elanor.sci.muni.cz
X-Mailer: Windows Eudora Version 1.4.3
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Hi folks,

could anyone help (directly or by advice) with finding plastic (PE probably)
tubing for ink circulation in an old (1988) INTEGREX Colourjet 132 ink jet
printer? Mine has been fused by a hot IC and the company servicing LINK /
OXFORD / CamScan products here is unable to replace the molten tubing.

Thanks in advance,

Petr Sulovsky







~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

U M M U
U M M M M U Dr. Petr Sulovsky
U M M M M U Dept. of Mineralogy,
Petrology and Geochemistry
U M M M M U Faculty of Science
U M M M M U Masaryk University
U M M M M U Kotlarska 2
U M M M M U 611 37 BRNO
U M M M M U Czech Republic
U M M M M U Phone + 42-541129231
U M MM M U Fax + 42-541211214
U M M M U e-mail: sulovsky-at-sci.muni.cz
U U
UUUUUUUU

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~





From: Larry Allard :      allardlfjr-at-ma160.ms.ornl.gov
Date: 21 Jun 1995 16:51:05 U
Subject: Digital TEM imaging

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Message-ID: {n1408368857.60343-at-ma160.ms.ornl.gov}

Subject: Time: 4:06 PM
OFFICE MEMO Digital TEM imaging Date: 6/21/95

Regarding John Mardinly's recent post:
John and I have been arguing about motorcycles longer than I care to
remember, so now maybe we can now have a new topic for argument:

Digital cameras offer many advantages over film usage in the electron
microscope. For high magnification, high resolution imaging, they offer
equivalent pixel resolution to the best photographic films. They provide
much higher dynamic range in the image, and they have essentially a linear
response function. They allow immediate processing of the image data so that
on-line decisions can be made regarding the data being taken (e.g. FFTs
provide information on the periodicities recorded in the image). However, if
you routinely take low magnification micrographs that cover large image
areas, and you enlarge those micrographs 20 times or more to make a poster,
you will have to "scale" the image (a function provided by e.g.
DigitalMicrograph from Gatan) to interpolate between pixels to avoid a
pixelated image (that looks like a bad image, but really is not). Edgar
Voelkl in my laboratory can provide an even better algorithm for image
scaling, as a plug-in for DM, for those who are intereste.

We have not had a single piece of film in our Hitachi HF-2000 in more than 2
years, and have not missed the hassle of film usage one single bit in that
period. I would not be surprised if a large majority of the laboratories
presently using digital imaging on their electron microscopes have
essentially eliminated film usage completely for the convenience of digital
imaging. Remember, most publications routinely degrade your images anyway,
so your best film images end up looking pretty bad when they finally are
published.

Larry Allard







From: GVKM07A-at-prodigy.com (DR CHARLES A GARBER)
Date: Wed, 21 Jun 1995 13:05:51 EDT
Subject: Legal/Ethical Questions for listserver

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-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --

In response to the original posting by John Wheatley and the replies by
others now totalling seven:

Everyone answering thus far has been employed by some major research
university, in charge of major tax-payer funded instrumentation, in
most instances, granted by the federal government, probably on an NSF
grant of one type or another (or in some cases, NIH). I now enter the
foray, not as a university faculty or staff member but as an owner of
one of those "service" laboratories referenced on more than a few
occasions. Just for the record, Structure Probe in an independent
laboratory offering laboratory analytical services to clients primarily
in industry. I am rather proud, in fact of what I did in 1970: With
private capital I risked everything that I had in this world and
against the advice of just about everyone who knew me at that time,
purchased the very first JEOL JSM U3 imported into the USA. My intent
was to offer a laboratory analytical service based on this new SEM.
Not one drop of government money, federal, state or otherwise, was used
in the formation of my firm. This "risk" investment also created jobs,
virtually from the very beginning, several of them of the higher paying
technical professional type.

And from this "seed" SEM, the company has grown to include the SPI
Supplies division and a work force of about 25 persons, many of them
highly trained professionals in the EM field.

I am personally astonished with the way one can rationalize as being
acceptable what is fundamentally an improper if not outright illegal
activity. This is nothing new to me since I have had to deal with this
"problem" now for over twenty five years. I have been invited to
testify on five different occasions before various committees of the
United State Congress to explain what it is like to be a small business
person in the United States and have as your main competitors
organizations who get their equipment given to them for free on a grant,
pay no corporate net income taxes at the federal, state, or local
levels, are free from their state's sales and use tax act, can import
equipment duty free, just to name a few of the unfair advantages. So
far I have not heard one single person say one word about the fairness
of it all. I have heard only that if the university charges
"commercial rates" then all will be OK and perceived to be "fair".

Another thing that astonishes me is that there has not been one mention
about NSF Important Notice 91. Now I don't know whether that is
because no one knows about it, or whether like 55 miles an hour speed
limits, people tend to ignore it, hoping they don't get caught. But
surely that should not be the policy of those employed in professional
positions of responsibility in some of our nation's leading
universities. Most of the commercial activities presented in the other
postings would be prohibited by NSF Important Notice 91 (I am less
familiar with NIH policies).

I have prepared a discussion on this issue but it is too long for a
posting on this listserver. Anyone interested in receiving what I have
prepared should e-mail me their request.

The bottom line, however, is that there should never be any situation
where a university's instrumentation would be used in the competitive
marketplace irrespective of what price is being charged. NSF Important
Notice 91 is very clear on that point. Price is not mentioned once
because price is irrelevant to the discussion. It would be a rare
instance, if ever, that it would be proper, legally or ethically, to
use equipment owned by any nonprofit or not-for-profit (or other type
of public entity), in competition with for profit tax-paying
organizations. No "spin doctor" can ever make it "kosher", it is just
plain wrong.

Chuck

======================================================
Charles A. Garber, Ph. D.
President
SPI SUPPLIES Div. of Structure Probe, Inc.
PO BOX 656
West Chester, PA 19381-0656 USA

Ph: 1-(610)-436-5400
1-(800)-2424-SPI

FAX: 1-(610)-436-5755

e-mail: GVKM07A-at-prodigy.com
SpiSupp-at-aol.com [SPI Customer Service e-mail box]
======================================================





From: BARRY PYLE :      umbbp-at-msu.oscs.montana.edu
Date: Wed, 21 Jun 1995 17:08:16 MDT
Subject: Image Analysis Software

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June 21, 1995

Does anyone have information on an image analysis software package available
from (or at least developed for) the National Institutes of Health? I have
heard that this package is extremely good and best of all it should be in the
public domain or available for minimal cost. I believe the program was written
for use with MacIntosh based systems.

Hope to hear from someone! Barry Pyle, Microbiology Department,
Montana State University - Bozeman.




From: richard.easingwood-at-stonebow.otago.ac.nz (Richard Easingwood)
Date: Thu, 22 Jun 1995 17:03:38 +1100
Subject: Dye sub printers

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Some 6 months to a year ago there was quite a lot of discussion on this
listserver about dye sublimation printers. We are hoping to purchase either
a slow-scan CCD camera or Accuview-type camera for a new TEM and need some
way of getting hardcopies as close as possible to photographic quality. I
still have most of the messages on this topic that were sent over the last
year but wondered if anyone would like to send me brief comments of their
own experiences with these printers. I suspect that the technology is
improving rapidly and what was state-of-the-art a year ago is probably now
superceeded. Your personal opinions or experiences (good or bad) with ANY
dye sub. printer models would be gladly received. I will treat any replies
which are sent directly to me confidentially.

Thanks in advance.

Richard E


Richard Easingwood
South Campus Electron Microscope Unit
Otago Medical School
PO Box 913
Dunedin
NEW ZEALAND

Telephone: 64-03-479 7301
Facsimile: 64-03-479 7254

"The southernmost electron microscope unit in the world"






From: Clint E. Young :      clintey-at-unixg.ubc.ca
Date: Wed, 21 Jun 1995 23:00:45 -0700 (PDT)
Subject: Macintosh Imaging Software

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Dear Barry,

The best (free) imaging software package for the Macintosh is--without a
doubt--NIH Image written by Wayne Rasband.

The following is taken from the introduction of the NIH Image manual:

"NIH Image is a public domain image processing and analysis program for
the Macintosh. Briefly, it can acquire, display, edit, enhance, analyze,
print and animate images. It reads and writes TIFF, PICT, PICS and
MacPaint files, providing compatibility with many other applications,
including programs for scanning, processing, editing, publishing and
analyzing images. It supports many standard image processing functions,
including contrast enhancement, density profiling, smoothing, sharpening,
edge detection, median filtering, and spatial convolution...

Image supports Data Translation and Scion frame grabber cards for
capturing images or movie sequences using a TV camera. Acquired gray
scale images can be shading corrected and frame averaged."

You can acquire this freeware program via anonymous FTP:
ftp://zippy.nimh.nih.gov/pub/image/

-----------
There are, however, many other imaging software packages. For starters,
you should take a look at the following web page:

http://www.msi.umn.edu/SciVis/Packages/packages.html

Hope this helps.

Clint Young, B.Sc.
Department of Psychiatry
Universtity of British Columbia




From: sorin.lazarescu-at-scf.fundp.ac.be (Sorin Lazarescu)
Date: Thu, 22 Jun 1995 11:43:07 +0200
Subject: NIH Software

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--========================_23134430==_
Content-Type: text/plain; charset="us-ascii"

"NIH Image" is the MacIntosh based image analysis software you asked
It is a shareware indeed and it requires a floating-point chip, or the
"Software FPU". You can dowlod it from any info-mac archive . This is one
of the many other lcations:

ftp://src.doc.ic.ac.uk/packages/info-mac/_Graphic/Utility/nih-image-152.hqx.gz

There is also an NIH Image mailing list. It was set up by a group in the Soil
Science Department at the University of Minnesota. To subscribe, send a
message containing the line "subscribe nih-image {your name} " to
soils.umn.edu.

In case if your mail has the attachment facility, double-click, in the
attachment message, on each of the followings items: "nih-image157.sea";
"nih-image157_docs.sea"; "SoftwareFPU 3.01." .



--========================_23134430==_
Content-Type: application/mac-binhex40; name="nih-image157.sea"
Content-Disposition: attachment; filename="nih-image157.sea"

(This file must be converted with BinHex 4.0)



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Content-Type: application/mac-binhex40; name="nih-image157_docs.sea"
Content-Disposition: attachment; filename="nih-image157_docs.sea"

(This file must be converted with BinHex 4.0)



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Content-Type: application/mac-binhex40; name="SoftwareFPU_3.01.sea"
Content-Disposition: attachment; filename="SoftwareFPU_3.01.sea"

(This file must be converted with BinHex 4.0)



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**********************************************************
Sorin Dan LAZARESCU e-mail:
sorin.lazarescu-at-scf.fundp.ac.be
F.U.D.P. dept de Physique-LASMOS phone : +32 (0)81 72 47 13
61, rue de Bruxelles +32 (0)81 72 47 18
B-5000 Namur BELGIUM fax : +32 (0)81 72 47 07
**********************************************************



--========================_23134430==_--





From: Bram Koster :      koster-at-schubert.biochem.mpg.de
Date: Thu, 22 Jun 1995 13:10:44 -0600
Subject: Digital TEM imaging

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I second Larry's comments regarding the advantages of CCD cameras. Our
Phlips CM200FEG is also equipped with a cooled 1024x1024 CCD camera. We also
are
very happy with digital data recording and have not put in film in more than 2
years. Our CM200FEG is used for a specific application (automated tomography)
for which the digital images of the CCD are essential.

However, the 1024x1024 CCD camera is not a convenient recording device for ALL
applications when we compare it to film, which can record images with more than
10000x10000 pixels. For example, high resolution images of large specimen areas
are crucial for imaging large structures, as well as for judging, documenting,
optimizing, etc., (routine) preparations of (negatively stained) biological
molecules. Of course, one could partly overcome the small image size by a
'montage' of a spot-scan series of 10x10 CCD images into one large digital
image of 10000x10000 pixels. When you do this, you will find the required data
collection time, as well as the required diskspace for --ONE-- such a large
image (200 MByte), rather inconvenient compared to just making an exposure on
film.

As mentioned above, we use on our CM200FEG exclusively a CCD camera, but the
other three microscopes in our group heavily depend on image recording on FILM
of large specimen areas with high resolution.

On Jun 21, 4:51pm, Larry Allard wrote:
}
} Digital cameras offer many advantages over film usage in the electron
} microscope. For high magnification, high resolution imaging, they offer
} equivalent pixel resolution to the best photographic films. They provide
} much higher dynamic range in the image, and they have essentially a linear
} response function. They allow immediate processing of the image data so that
} on-line decisions can be made regarding the data being taken (e.g. FFTs
} provide information on the periodicities recorded in the image). However, if
} you routinely take low magnification micrographs that cover large image
} areas, and you enlarge those micrographs 20 times or more to make a poster,
} you will have to "scale" the image (a function provided by e.g.
} DigitalMicrograph from Gatan) to interpolate between pixels to avoid a
} pixelated image (that looks like a bad image, but really is not). Edgar
} Voelkl in my laboratory can provide an even better algorithm for image
} scaling, as a plug-in for DM, for those who are intereste.
}
} We have not had a single piece of film in our Hitachi HF-2000 in more than 2
} years, and have not missed the hassle of film usage one single bit in that
} period. I would not be surprised if a large majority of the laboratories
} presently using digital imaging on their electron microscopes have
} essentially eliminated film usage completely for the convenience of digital
} imaging. Remember, most publications routinely degrade your images anyway,
} so your best film images end up looking pretty bad when they finally are
} published.
}
} Larry Allard
}
}
} -- End of excerpt from Larry Allard



--

Bram

------------------------------------------
Dr.ir. Abraham (Bram) J. Koster
Max Planck Institute for Biochemistry
Department Molecular Structure Biology
Am Klopferspitz 18A
D-82152 Martinsried (near Munich), Germany
tel: (49) 89 - 8578 2632
fax: (49) 89 - 8578 2641
email: koster-at-schubert.biochem.mpg.de
------------------------------------------




From: GVKM07A-at-prodigy.com (DR CHARLES A GARBER)
Date: Thu, 22 Jun 1995 08:08:39 EDT
Subject: Legal/Ethical Questions

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-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --

Thank you for requesting a copy of what I drafted up but which clearly
was too long to put out on the listserver. I offer this document not to
step on anyone's toes or to get people upset with me but to offer my
views, because what I see happening and going on is just plain wrong.

Let me start out by saying I believe that I am very qualified to
comment on this question. In 1970 I resigned my job at DuPont and
without a single client "lined up" in advance and armed only with my
belief that the SEM was going to be the "growth instrument of the
decade", I leveraged everything I had, purchased the very first JEOL
JSM U3 imported into the USA (it was serial #1) and started my own
business. I hired people at professional levels, paid good
professional level wages and also my full share of taxes, and
contributed, I think in a meaningful way to the economic development of
the region I live if not also the nation.

So it came as a real surprise to find out, in 1970, that there were
those persons, primarily at universities, who thought that they could
use their "own" university EM equipment as if such facilities were
their own to use for their own private so-called "consulting"
businesses. In just about any other circumstance, doing this would be
called white collar stealing, for which people do hard time, however
in "university-speak" this is called "consulting" and is somehow
presented to the students as "admirable behavior". One has to wonder
about the professor as role model and the effect on students!

Yes, on a regular basis, I too am hired as a consultant, and am paid a
daily fee, and the principal ability for me to do that is what is
between my ears. Not in "university-speak" but in plain English, this
is what most of the world calls "consulting". But when I leave my
client with samples under my arm to be characterized on some of our own
in-house instrumentation, then the principal ability to do it involves
access to the very expensive EM instrumentation and that is called by
everyone else, a "laboratory service". In any language other than
"university-speak", there is a clear difference between "consulting"
and "laboratory services".

Now I don't think that one has to be a rocket scientist to appreciate
that such use of the university's facilities is fundamentally wrong,
not just because of the potential for interference with legitimate
educational objectives but also because of the unfair competition it
presents for small firms like my own. After all, how could any
legitimate business compete long term with a competitor who did not pay
any taxes, did not have to pay for their equipment (it was granted via
NSF or some other funding agency), was exempt from import duties, and
as if this was not enough, they had a myriad of other benefits not
available to any for-profit tax-paying firm, either large or small.

Now those on the other side of the fence have argued that people with
my perspective are trying to "stop universities from doing research and
educating students" but nothing could be further from the truth. If
some commercial firm wants to give money to a university department for
work that is going to contribute to educational objectives, that is,
work that is basic and fundamental in nature, suitable for publication
in a student's thesis, and is intended for prompt publication in a
reputable scientific journal, then that is a win-win-win situation for
everyone. Surely I have no objection to that kind of activity and I
don't know of anyone else who would either. Indeed I would be the
first in line if one needed someone to speak in favor of the need for
universities to maintain a top notch ability to educate the researchers
of tomorrow, which can only be done if a top notch ability to conduct
research is maintained.

The objection is strictly to work being done that falls outside of that
which enhances educational objectives. The objection is with work that
either is not suitable for publication at all or else is proprietary in
nature and the client firm just would not want to see it published.
Why? Because he does not want to lose his competitive advantage and
give away his results to his competition. Unreasonable? I sure
wouldn't think so, after all he has a responsibility to the
shareholders to protect the company's assets and intellectual knowledge
is certainly as important an asset as any. So this then, by definition,
is the kind of work that more appropriately ought to be getting done in
the private for-profit sector and not in a university laboratory.

With the passing of time, some university administrations found they
had to do something to put a better face on what was going on in some
of their laboratories, and this led to some really creative approaches,
resulting in the formation of "industrial affiliate" programs, or
"industrial liaison" programs. Now I don't mean to paint all such
programs with the same brush, but I know of enough that are indeed
nothing more than shams to put a better face on what is basically an
inappropriate use of the equipment and instrumentation.

Let me give another example. A number of universities have set up not-
for-profit "research" institutes which becomes the official
"contractor" for outside users. The client companies contact with the
research institute, and the faculty for their consulting have their
consulting fees funnelled through the research institute. It then is
the research institute that, technically, does the contracting with the
university for access to the university's instrumentation. I am aware
of deans of engineering at such universities who will swear up and down
on a stack of bibles that "oh, no, we do absolutely no work directly
for private companies". They tell that to their state legislators.
They tell that to anyone who might ask. And some how, in their
convoluted way of thinking, they conclude that "oh, no, we are not
competing with private firms" and they think that such a sham kind of
structure makes them good citizens and operating within accepted norms.

In 1980, after complaints from numerous private testing and analytical
laboratories from across the USA, the NSF issued Important Notice 91
which essentially drew for the first time a line between what was and
was not appropriate usage of NSF funded instrumentation for outside
commercial users. A special committee, made up of top NSF officials
(the late Dr. George Pimentel played a key role in the formulation of
the final document as did also Dr. Donald Langenberg, now Chancellor of
the University of Maryland system), lawyers representing the NSF (Mr.
Charles Herz who is still at NSF in that same capacity), members of the
independent laboratory community (including myself) plus some other
knowledgeable people came up with the first draft of NSF Important
Notice 91. Some additional months passed before the document was ready
to be issued in its final form. And today, each and every NSF grantee
institution supposedly signs off each year certifying that they are in
full compliance with NSF Important Notice 91. I realize that NSF has
not done an especially good job in publicizing the existence of NSF
Important Notice 91, possibly the reason why none of the prior postings
on this subject have referenced Important Notice 91. But NSF Important
Notice 91, like it or not, has been the "law of the land" since its
release in 1980.

I decided to do a public rather than a private response to the John
Wheatley posting. Ethical values know no national boundaries, and in
any case, the issue is being discussed in other countries as well. None
of us should ever fear spreading the gospel of high ethical values and
conduct in the EM laboratory. Everyone does a lot of talking about
ethical values but did you ever try to write down a definition? The
dictionary defines "ethics" as "conforming to the standards of conduct
of a given profession or group". I like to extend that definition so
that when one talks of ethical values, it means that we are behaving
according to the definition EVEN WHEN NO ONE IS LOOKING AND THERE IS NO
CHANCE OF GETTING CAUGHT IF WE DON'T. In any case, behavior of faculty
in a university has to be held to a standard that is at the highest,
not the lowest common denominator. You have no idea what I have
learned from students over the years as to what goes on in some
departments. Or course students are caught between the rock and the
hard place: Sure they like the extra money that comes from running an
SEM or TEM for their advisor, when no one is looking, in the wee hours
of the morning. On the other hand, they have a good sense that
something is not quite right, they might not be able to exactly
understand or verbalize exactly why it is not right, but it is not
wasted on them that the activity being done is coming in the back door
when no one is looking. Is this really the message students should be
getting as they are being prepared for their future lives as a
professional? Like father, like son, some of the biggest academic
"entrepreneurs" in universities are themselves the students of other
"famous" entrepreneurs. Actually I am using the word "entrepreneur" as
used in the language of "university-speak". Real world entrepreneurs
take major financial risks. What kind of a financial risk does one
take when they accept samples and run them on their equipment as part
of their "consulting" business? What kind of risk does one take when
they "double dip", that is, are being paid by the university and for
the same hours worked, are being paid by the client company? Now maybe
this does not actually go on, maybe the "consulting" work is being done
on vacation time, or whatever, but it is the perception of the students
that count, isn't it? If perception is 98% of reality, the students,
or at least some of them, see their professors as something less than
honest people. If there are any doubts about this, stick around our
exhibit booth at MSA and listen for yourself what student have to say
when they drop off their resumes.

Another point about ethical values and that is that they are not the
kind of thing that you leave at the door when you walk into a different
room. Someone with high ethical values in one part of their life
probably has high ethical values in all parts of their life. The
conduct of high quality research demands honesty and that the
researchers have the highest of ethical values. One has to wonder to
what degree research results can be trusted when obtained by someone
who sneaks samples in the back door when no one is looking. Or when
such convoluted rationalizations are proposed so that somehow,
activities that are fundamental wrong and unfair, are somehow made to
seem right and fair.

I have not posted any prices and I certainly hope no one else does
either since that would come dangerously close to violations of the
anti-trust laws. I would respectfully suggest that no one even think
about posting "your price structure for industrial jobs - proprietary
or otherwise". We may not be lawyers, but surely we should know enough
to not be asking others to submit prices for the purpose of comparing
"competitive" prices. The threat of doing "hard time" for doing that
is usually enough to keep private companies from even thinking about
doing something like that! It should be enough to keep us from doing
that kind of thing as well.

The State of Wisconsin (Wise posting) can pass all the legislation it
wants and I realize Madison is some distance from the nation's capitol,
however it is not that far away that it can out run the intent of
federal law and regulation. Today, NSF Important Notice 91 is the law
of the land and it became necessary only after it became very very
clear that universities could not police themselves in terms of the way
some of their instrumentation facilities were being commercialized,
either officially or unofficially..

Another point has to do with the conclusions made by some that "we are
not in competition with private companies". It is really the fox
guarding the chicken coop saying the chickens are safe. No one seems
to consider that new would-be start up firms are literally preempted
from the market place because prospective entrepreneurs know full well
that an investment in such a firm would fail because they could not
compete. I myself have been turned down by leasing companies because
they ask "what if the University of XXXXXXX nearby decides to buy one
and compete with you". Of course I would fail and they know it. So I
can't get my financing. And the instrumentation does not get
purchased. And the jobs never get created. The university is always
going to be in competition when work outside of the scope of real
educational objectives is involved.

This is surely a subject that is of critical interest to anyone
involved with electron microscopy. Whether we are in academia,
industrial laboratories, or even vendors like myself, developing, doing
research, and manufacturing and distributing products to the industry,
we all have a vested interest in having a healthier rather than weaker
market place. Maybe some evening at MSA a meeting room could be found
where a panel discussion could be arranged, so that everyone might gain
a better appreciation for the other side's position on this subject,
with the ultimate goal being that we would be working together for our
mutual benefit on some of these issues.

Chuck

======================================================
Charles A. Garber, Ph. D.
President
SPI SUPPLIES / Division of Structure Probe, Inc.
PO BOX 656
West Chester, PA 19381-0656 USA

Ph: 1-(610)-436-5400
1-(800)-2424-SPI

FAX: 1-(610)-436-5755

e-mail: GVKM07A-at-prodigy.com
SpiSupp-at-aol.com [SPI Customer Service e-mail box]
======================================================





From: Jay Jerome :      jjerome-at-isnet.is.wfu.edu
Date: Thu, 22 Jun 1995 10:21:04 -0400 (EDT)
Subject: Re: Legal/Ethical Questions for listserver

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Charles-
I would be very interested in receiving a copy of your posting. I will
supply it to our legal people here. For the time being, however, their
interpretation differs from yours.
As for the ethical issues. At many Medical Schools these days, we as
directors of laboratories are essentially independent entrepreneurs. My
laboratory only remains open as long as it generates enough capital to
pay the salaries and fringe of my technical people, service and operating
costs of my microscopes, and that portion of my time required to run the
facility. If this does not occur, I will be shut down. It is the same
with all of our support facilities (NMR, Flow cytometry, etc.). That is the
reality of the world these days. That I accomplish this using a mixture
of hospital service, grant support (many small businesses also use
government grants and contracts), and service to in-house and outside
is irrelevant as long as what I charged for that work represents fair
cost for doing the work. If I use University of Government money to pay
the service contracts and use the benefit to undercut prices THAT IS
UNFAIR, UNETHICAL, and ILLEGAL. If I generate business because I am close
to the user, have expertise he requires, or because he trusts my product
more than a competitors- that seems to me to be fair competition. As long
as the playing field is level I see no reason not to offer service to the
outside community. We don't do a lot of it, but what we do is important
to the financial well being of my facility. After all, many small
businesses regular try to get me to use them for my cell culture needs or
to produce monoclonal antibodies and oligonucleotides in direct
competition with our In-House tissue culture laboratory and probe
generating facilities.

Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-isnet.is.wfu.edu *
**************************************************************





From: Greg :      GREG-at-umic.umic.sunysb.edu
Date: Thu, 22 Jun 1995 10:50:47 EST5DST
Subject: Digital Imaging

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I am interested in digitizing a JEOL 5300 SEM, Tungsten filament,
with a passive system. This is nonbeam control. The SEM is
in a core facility and both biological and materials are viewed. Has anyone
up gradedan SEM with after market equipment and software? Are you happy
with the results?

Greg-at-umic.umic.sunysb.edu
SUNY at Stony Brook
Univ. Microscopy Imaging Center





From: Sally Shrom :      sally-at-retina.anatomy.upenn.edu
Date: Thu, 22 Jun 1995 11:28:20 -0400 (EDT)
Subject: TEM/Bio/EPON

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X-Nupop-Charset: English

Thanks to Stacie Kirsch at Electron Microscopy Sciences I have
finally solved my epon problem. I had trouble with my immuno experiments
when I used DAB with epon. I now know that the tissue looks great with
the epon from EMS. The problem is with the DAB ( not from EMS). Thanks
for all who responded to my original epon posting. I learned a lot...Sally




From: emccjb-at-ttuhsc.edu (Charles J. Butterick)
Date: Thu, 22 Jun 1995 11:28:20 -0400 (EDT)
Subject: Forensic/Balllistic Lab Needed

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Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
To: MICROSCOPY-at-AAEM.AMC.ANL.GOV

Greetings,
My apologies in advance to anyone who thinks my question is
inappropriate for this List.
Our local police asked me if I had the microscope that would enable
them to examine two bullets simeltaneously. I don't. They would send it to
Austin but Austin has a 6 month backlog and an answer is needed in 10 days
for a warrant.
Does anyone know of a lab does ballistics comparison with a
microscope? Please respond to me directly and not to the list.
Thanks.



Charles J. Butterick (Chuck)
Electron Microscopy Center
Department of Cell Biology
and Biochemistry
Texas Tech University Health
Sciences Center
3601 4th Street
Lubbock, Texas 79430

vox (806) 743-1633
fax (806) 743-1219
email emccjb-at-ttuhsc.edu or
chuck-at-micron1.lubb.ttuhsc.edu






From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-AAEM.AMC.ANL.GOV
Date: Thu, 22 Jun 1995 9:17:23 -0500 (CDT)
Subject: Do not post Software to the List

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G'day All

Yes, I saw the posting with the Software attachment
and have sent a note to the individual. Just as
a reminder to everyone. DONOT post code/programs/images
or any file longer than a few pages of text. If you
have something large that you wish to distribute
send it to me seperately and I will put it up
on the Anonymous FTP server which services the
Public Domain Software Libraries here at ANL.

You can then send your message with the information
that the data is on the FTP Server and anyone who
wants it can then retrieve it at their convenience.

Sorry, folks but I DONOT prescreen the postings.

Nestor
Your Friendly Neighborhood SysOp




From: ychen-at-macc.wisc.edu
Date: Thu, 22 Jun 1995 13:31:54 -0500
Subject: No big file on net, please!

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Message-Id: {25062213272294-at-vms2.macc.wisc.edu}

Dear netter,
Please send big file off net. Otherwise it will jam the whole net.
Thank you!

Ya Chen



Ya Chen

=========================================================================
\ / Assistant Researcher-Cryo/SEM Coordinator TEL : 608-263-8481
\ / __ Integrated Microscopy Resource (IMR) FAX : 608-265-4076
\/ / / University of Wisconsin-Madison
/ / / 1675 Observatory Drive #167 Email:YChen-at-macc.wisc.edu
/ /__/_ Madison, WI 53706 Email:chen-at-calshp.cals.wisc.edu
=========================================================================






From: sorin.lazarescu-at-scf.fundp.ac.be (Sorin Lazarescu)
Date: Thu, 22 Jun 1995 18:40:17 +0200
Subject: Apologize

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I am terribly sorry for the inconveniences I've caused to all of you, today.
That happend when sending, (to someone, in private), some image analysis
software, I forgot to disable the { {Cc: microscopy-at- aaem.amc.anl.gov} } .
I have no excuse and, once more, I am very confused and I apologize for the
mess I've done to all of you.

**********************************************************
Sorin Dan LAZARESCU e-mail:
sorin.lazarescu-at-scf.fundp.ac.be
F.U.D.P. dept de Physique-LASMOS phone : +32 (0)81 72 47 13
61, rue de Bruxelles +32 (0)81 72 47 18
B-5000 Namur BELGIUM fax : +32 (0)81 72 47 07
**********************************************************






From: Glen Macdonald :      glenmac-at-u.washington.edu
Date: Thu, 22 Jun 1995 12:02:27 -0700 (PDT)
Subject: Re: Digital Imaging

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X-Sender: glenmac-at-homer01.u.washington.edu

Greg,
We have been happy with results obtained by simply running a BNC
cable from our JEOL's video out port to an 8-bit framegrabber board in a
Macintosh computer. We capture with NIH-Image, timing the capture when
the scan reaches the bottom of the screen. This is for biological
samples, and has given publication quality images up to fairly high mags,
at least on our samples.
When we get enough other users interested, then we'll probably buy a system
that will give us full computer interface/control of the SEM.
I could send you the tiff files.

Regards,
Glen

On Thu, 22 Jun 1995, Greg wrote:

} I am interested in digitizing a JEOL 5300 SEM, Tungsten filament,
} with a passive system. This is nonbeam control. The SEM is
} in a core facility and both biological and materials are viewed. Has anyone
} up gradedan SEM with after market equipment and software? Are you happy
} with the results?
}
} Greg-at-umic.umic.sunysb.edu
} SUNY at Stony Brook
} Univ. Microscopy Imaging Center
}
}




From: Greg :      GREG-at-umic.umic.sunysb.edu
Date: Thu, 22 Jun 1995 15:17:16 EST5DST
Subject: Re: Digital Imaging

Contents Retrieved from Microscopy Listserver Archives
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} Date: Thu, 22 Jun 1995 12:02:27 -0700 (PDT)
} From: Glen Macdonald {glenmac-at-u.washington.edu}
} To: Greg {GREG-at-umic.umic.sunysb.edu}
} Cc: microscopy-at-aaem.amc.anl.gov
} Subject: Re: Digital {SEM} Imaging

} Greg,
} We have been happy with results obtained by simply running a BNC
} cable from our JEOL's video out port to an 8-bit framegrabber board in a
} Macintosh computer. We capture with NIH-Image, timing the capture when
} the scan reaches the bottom of the screen. This is for biological
} samples, and has given publication quality images up to fairly high mags,
} at least on our samples.
} When we get enough other users interested, then we'll probably buy a system
} that will give us full computer interface/control of the SEM.
} I could send you the tiff files.
}
} Regards,
} Glen
}
} ?
} }
} } Greg-at-umic.umic.sunysb.edu
} } SUNY at Stony Brook
} } Univ. Microscopy Imaging Center
} }
Glen,
Please send the TIFF images. It is good to see how these
systems work in the real world.--Thanks, Greg
} }
}





From: jfmjfm-at-umich.edu (John F. Mansfield)
Date: Thu, 22 Jun 1995 11:03:22 -0400
Subject: Re: HREM Shareware

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X-Sender: jfmjfm-at-srvr5.engin.umich.edu
Message-Id: {v02120d0dac0f2cf8d75a-at-[141.213.21.13]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

It strikes me that this is entirely counterproductive to the effort that we
have put in over many years maintaining the Electron Microscopy and
Microanalysis Public Domian Library and the Microbeam Analysis Software
library. try the following:

ftp://www.amc.anl.gov/AMC-3/ANLSoftwareLibrary/
ftp://freebie.engin.umich.edu/pub/MSA+MAS/

Those wishng to contribute should send mail to zaluzec-at-aaem.amc.anl.gov for
the EMMPDL and to john.f.mansfield-at-umich.edu for the MASSL.


} Within our homepage (http://risc1.numis.nwu.edu/internet/lab.html) I
} am going to start collecting shareware for HREM. The primary categories that
} I am thinking of are:
} a) Semper (5 or 6) library programs or run programs.
} b) X-windows based routines such as simple CTF graphers.
} c) Other X-windows type programs.
}
} I can take PC based routines, with many reservations Mac-based programs
} but since we are primarily unix/workstation focussed this will be the
} exception.
}
} Contributions can be sent via anonymous ftp to the directory contrib
} at risc1.numis.nwu.edu; any requests for further information or clarification
} can be emailed to me.

John Mansfield
North Campus Electron Microbeam Analysis Laboratory
413 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (313)936-3352 FAX (313)936-3352
jfmjfm-at-engin.umich.edu, John.F.Mansfield-at-umich.edu
or jfmjfm-at-umich.edu they all reach me!
URL: http://www.engin.umich.edu/~jfmjfm/jfmjfm.html






From: bell-at-medcolpa.edu (Ted Bell)
Date: Thu, 22 Jun 1995 17:49:47 -0400
Subject: need to replace an outdated bioquant system

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We currently have an old bioquant system capable of flourescence and want to replace it with a state of the art system, which is not from Bioquant. First off, are there any recommendations, especially in terms of cameras and frame grabbers?

1) Currently, the computer is 33MHz Gateway 486? Is it worth replacing with a Pentium or second-generation PowerMac?

2) Which kind of camera is best, digital or analog? What the are the advantages and disadvantages of each?

3) If we go with an analog camera and frame grabber, does anyone know if any company is now offering PCI frame grabber cards for the second-generation Power Macintosh yet?






From: gfan-at-ucsd.edu (by way of jpawley-at-macc.wisc.edu (James Pawley))
Date: Thu, 22 Jun 1995 12:21:16 -0600
Subject: Re: Digital TEM imaging

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Message-Id: {9506221701.AA19386-at-sage.macc.wisc.edu}
X-Sender: jpawley-at-vms2.macc.wisc.edu
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

} Nice balanced answer Gary. I would only add that this reduction in data
} resolution (below what one might expect from the "pixel area" of the CCD),
} is a strong function of voltage (higher is worse).
}
} One can use thinner phosphors to limit the effect but this reduces the
} signal level and is not too effective if fiber-optica couplings are used
} because the FO scatters many of the electrons passing down through the
} phosphor back up through it again, on a different trajectory.
}
} Likewise the dynamic range of the entire detector is far less that that of
} the (cooled?) CCD camera itself. The phosphor thickness is usually chosen
} so that each beam electron produces, say 10 electron/hole pairs in the CCD.
} This reduces the CCDs dynamic range by 10x.

I agree with you on these points.

} If you really want wide dymanic range, and digital imaging with a faster
} record-cycle time (between recorded images) than a CCD (which can take many
} seconds to read out), try a Fuji Image Plate system...
}
} Jim Pawley

and wait for a few days to see the images. Incidentally, there will be
a talk on the imaging plate at the symposium I mentioned.

Yes, readout time is a problem at the present. We are trying
a readout scheme that is much faster.

Just remind you that you replied to me, not the list.

Gary Fan
UC San Diego








From: ldm2-at-apollo.numis.nwu.edu (L.D.Marks)
Date: Thu, 22 Jun 1995 17:55:41 -0500
Subject: Re: HREM Shareware

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The purpose of my email was not an attempt to kill either
EMMPDL or MASSL but completely different. The idea is to collect
primarily X-based routines for HREM and other miscellaneous such as
Semper code and libraries. I believe that there has been a disturbing
trend over the last ten years for heavy-duty programs for HREM to
be written on Government funding then sold commercialy. These programs
should be much easier and cheaper than they are (} $5000 in many cases).
I intend to donate NUMIS to this (at least the multislice and imaging
parts) as soon as I can rewrite for X-windows and thereby escape a
licensing agreement.

The more sites, the more software, the better!




From: Elinor Solit :      cambrex-at-world.std.com
Date: Thu, 22 Jun 1995 17:28:02 +0059 (EDT)
Subject: Re: Forensic/Balllistic Lab Needed

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Chuck,

Any of the four light microscope manufacturers can probably do this for
you on an application basis. Numbers at hand are:

Carl Zeiss (ask for Ernest Keller) 800-356-1090
Olympus 800-446-5967
Leica 800-248-0123

Hope this helps.

Ellie Solit
"The Microscope Book" Call me if more assistance needed. 800-440-0311

On Thu, 22 Jun 1995, Charles J. Butterick wrote:

} Greetings,
} My apologies in advance to anyone who thinks my question is
} inappropriate for this List.
} Our local police asked me if I had the microscope that would enable
} them to examine two bullets simeltaneously. I don't. They would send it to
} Austin but Austin has a 6 month backlog and an answer is needed in 10 days
} for a warrant.
} Does anyone know of a lab does ballistics comparison with a
} microscope? Please respond to me directly and not to the list.
} Thanks.
}
}
}
} Charles J. Butterick (Chuck)
} Electron Microscopy Center
} Department of Cell Biology
} and Biochemistry
} Texas Tech University Health
} Sciences Center
} 3601 4th Street
} Lubbock, Texas 79430
}
} vox (806) 743-1633
} fax (806) 743-1219
} email emccjb-at-ttuhsc.edu or
} chuck-at-micron1.lubb.ttuhsc.edu
}
}




From: Michael Rock :      merock-at-u.washington.edu
Date: Thu, 22 Jun 1995 09:24:24 -0700 (PDT)
Subject: Re: Dye sub printers

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Richard-
we have a Tektronix "Phaser II SDX" dye sub machine, the prices have come
way down over the past 2-3 years, the images are quite nice, and the
print speed is adequate. the only problems we have experienced are with
the mechanical paper feed, we get frequent paper jams. kind of annoying,
but with extensive cleaning, can be minimized.
-Mike

On Thu, 22 Jun 1995, Richard Easingwood wrote:

} Some 6 months to a year ago there was quite a lot of discussion on this
} listserver about dye sublimation printers. We are hoping to purchase either
} a slow-scan CCD camera or Accuview-type camera for a new TEM and need some
} way of getting hardcopies as close as possible to photographic quality. I
} still have most of the messages on this topic that were sent over the last
} year but wondered if anyone would like to send me brief comments of their
} own experiences with these printers. I suspect that the technology is
} improving rapidly and what was state-of-the-art a year ago is probably now
} superceeded. Your personal opinions or experiences (good or bad) with ANY
} dye sub. printer models would be gladly received. I will treat any replies
} which are sent directly to me confidentially.
}
} Thanks in advance.
}
} Richard E
}
}
} Richard Easingwood
} South Campus Electron Microscope Unit
} Otago Medical School
} PO Box 913
} Dunedin
} NEW ZEALAND
}
} Telephone: 64-03-479 7301
} Facsimile: 64-03-479 7254
}
} "The southernmost electron microscope unit in the world"
}
}
}




From: jpawley-at-macc.wisc.edu (James Pawley)
Date: Thu, 22 Jun 1995 18:38:19 -0600
Subject: Re: Digital TEM imaging

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Message-Id: {9506222318.AA20720-at-sage.macc.wisc.edu}
X-Sender: jpawley-at-vms2.macc.wisc.edu
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

} Nice balanced answer Gary. I would only add that this reduction in data
} resolution (below what one might expect from the "pixel area" of the CCD),
} is a strong function of voltage (higher is worse).
}
} One can use thinner phosphors to limit the effect but this reduces the
} signal level and is not too effective if fiber-optica couplings are used
} because the FO scatters many of the electrons passing down through the
} phosphor back up through it again, on a different trajectory.
}
} Likewise the dynamic range of the entire detector is far less that that of
} the (cooled?) CCD camera itself. The phosphor thickness is usually chosen
} so that each beam electron produces, say 10 electron/hole pairs in the CCD.
} This reduces the CCDs dynamic range by 10x.

I agree with you on these points.

} If you really want wide dymanic range, and digital imaging with a faster
} record-cycle time (between recorded images) than a CCD (which can take many
} seconds to read out), try a Fuji Image Plate system...
}
} Jim Pawley

and wait for a few days to see the images. Incidentally, there will be
a talk on the imaging plate at the symposium I mentioned.

Yes, readout time is a problem at the present. We are trying
a readout scheme that is much faster.

Just remind you that you replied to me, not the list.

Gary Fan
UC San Diego








From: Chris Jefferies :      chris-at-stowey.demon.co.uk
Date: Fri, 23 Jun 1995 00:03:14 GMT
Subject: Re: HREM Shareware

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In his message dated Thursday 22, June 1995 John Mansfield wrote :

} It strikes me that this is entirely counterproductive to the effort that we
} have put in over many years maintaining the Electron Microscopy and
} Microanalysis Public Domian Library and the Microbeam Analysis Software
} library.

I'm puzzled here. I don't see how an additional ftp site can be
*counter*productive.

Surely most of us would agree that an extra site spreads the load a little
and may well specialise in a slightly different area from existing sites.
This isn't to detract from the hard work (much appreciated BTW) that John M
and Nestor Z put into creating and maintaining their resources.

I already have Nestor's ftp site on the 'Microscopes and Microscopy' Web
pages. I'd missed John's (but I'll add it now). I'll certainly add the new
HREM site as well. The more the merrier I think. :-)

Regards to everyone from Bristol, UK

Chris
--
---------------------------------------------------------------------------
| Chris Jefferies E-Mail at home - chris-at-stowey.demon.co.uk |
| at work - chris.jefferies-at-bbsrc.ac.uk |
| Microscopy page {URL: http://metro.turnpike.net/jefferie/index.html} |
---------------------------------------------------------------------------




From: Roar Kilaas :      Roar_Kilaas-at-macmail7.lbl.gov
Date: 22 Jun 1995 18:12:29 U
Subject: Re: HREM Shareware

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Message-Id: {n1408277709.50174-at-macmail7.lbl.gov}
microscopy-at-aaem.amc.anl.gov
X-Mailer: Mail*Link SMTP/QM 3.0.0

Reply to: RE} } HREM Shareware

With respect to the comment about making an X-window image simulation package available, I would like to inform that such a program
already exists. It is called NCEMSS and is available from the National Center for Electron Microsocpy at the Lawrence Berkeley
National Laboratory. The program is written by Dr. Roar Kilaas and is currently available for the following platforms: SUN, SGI,
Dec Alpha (OSF & OpenVMS), DecStations, and the IBM RISC Series computers. All versions except the DecStation and IBM versions are
posted at the site:
http://ncem/NCEM/computer/software.html
The two missing versions will be added shortly.
Roar Kilaas
Phone : 510-486-4618
e-mail : Roar_Kilaas-at-lbl.gov





From: Alex Titkov :      ALEX-at-bunyip.ph.rmit.edu.au
Date: Fri, 23 Jun 1995 11:00:38 EST-10
Subject: SEM of fluoridated plastics

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Dear Microscopists,

We have a customer who wants to see the internal surfaces of light
pipes made of various materials. The relevant features are about 100
nm. The materials are fluoridated plastics (like teflon), but some of
them are unstable even at 130C.

We sputter coated the samples with gold (various thicknesses between
30 and 90 nm) and viewed them in a conventional SEM (W filament) at
15-25 kV keeping the beam current as low as possible (down to 30 pA).

The problem is that the samples with thin coatings are unstable under
the electron beam whereas thick coatings seem not to form a continuos
layer of gold on the plastic, resulting in an orange peel appearance
(200-300 nm dia. gold "islands"?).

We would appreciate any ideas about imaging such samples.

Thank you.
---
Alex Titkov
Electron Microscopy Unit
RMIT Applied Physics
GPO Box 2476V
Melbourne VIC 3001
AUSTRALIA
alex-at-bunyip.ph.rmit.edu.au
Voice:(03) 660 2205
Fax: (03) 660 3837




From: MicroToday-at-aol.com
Date: Thu, 22 Jun 1995 21:00:56 -0400
Subject: Digital vs. Film

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Groupus-
For those of you that receive our newsletter "Mircoscopy Today", I trust that
you will agree that the resolution, etc. of our micrographs and images are AT
LEAST as "good" as produced by ANY publication. And I continue to be very
interested in the topic of film vs. digital input..
As a side topic - should you be interested in producing printed copies of
your micrographs, etc., allow me to stress the importance of the printing
process:
1) An Al plate, with the image burned in from a negative, carries the ink to
the paper. A (large) quality press with a good operater is critical. If you
go to Econoprint, you will get good garbage. And high quality print shops
are expensive!
2) The image, from film or digital, is shot thru a dot-screen to produce
the negative. A 150, 180 or 200 line screen is appropriate for good work.
The higher, the better - assuming that the printing press can handle it. A
good press with a poor screen, or a good screen on a poor press, will result
in MUD.
As the result of a previous "thread" on this topic, I went to the
professional seperation house that I work with (NOT my printer) and asked
their advice - which was that 4" x 5" transparencies were the superior,
followed by 35 mm slides. Following were paper positives and digital. In
fairness to all, it is tough to "look" at the origional of a digital image
and compare it to the printed result.
With my continued interest in this topic, I presented the question to David
Scharf. Should you not be familiar with his work, I can only submit that he,
as a microcopist/photographer, has produced more nifty EM micrographs than
all of us together.
Dave advises that at the point a digital image is 48 bits deep, and in a 40
mbyte file, it generally becomes superior to film. Below that, film is
superior.
I fully appreciate that there are "computer jocks" out there that are very
excited over their results - and have opinions that will remain unchanged.
I, however, would like to continue to become "educated" on this subject and
would appreciate any contribution to my education.
Peace and All Kinds of Good Will,
Don Grimes, Microscopy Today







From: Michael Rock :      merock-at-u.washington.edu
Date: Thu, 22 Jun 1995 10:23:15 -0700 (PDT)
Subject: Re: Legal/Ethical Questions for listserver

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In response to Charles Graber and Jay Jerome- You both have very valid
points, and good reasons for speaking out.

First Jay- saying that your legal people have a different interpretation
than Chuck's point of view, is about as significant as saying O.J.'s
lawyers have a different interpretation than the prosecution lawyers.
Let's address what is "right" or "wrong", and understand that change is
the only constant.

Let's deal with this Important Notice 91, does it have any teeth?
How do we enforce it? or are rules just made to be broken? Or is this law
out of date, do we need to revise it for the times?

And second Chuck- Jay is correct when he states that this type of govt.
supported is happening all the time, it is a way of doing business,
keeping university/hospital labs open. I have worked in and managed several
labs where during "lean times", if not for "outside" revenue generating
projects, the lab would have been shut down. This does not make it OK,
but utilizing resources is very important.

Which brings me to my pet peeve, resource management in the EM industry.
Universities need to keep up with the ever changing technology, but at what
price? In academia many faculty seem to "need" a new EM every couple of years,
with each technological breakthrough, and equip it with all the latest
analytical equipment, but seem to forget who is paying for this. What is
the universities mission? to teach?, or to satisfy the whims of some very
spoiled individuals by supplying them with a new toy every time they cry
out?
Out there in the real world (industry), companies purchase equipment
when they can afford it, not "justify it" (academic lingo). Most of the
EM service labs are probably using equipment that is a decade or two old,
not a year or two. Most can't afford service contracts, and most...go broke.
Sure companies in the IC industry can purchase the top of the line
equipment, but they can afford it, it's part of what they need to do to
stay in business. Maybe they should be the leaders in this academic/industrial
partnership by opening up their facility for educational purposes instead of
placing the burden on the tax payers to buy the equipment and performing this
"consulting service"???

-Mike




From: mager-at-unixg.ubc.ca (Mary Mager)
Date: Thu, 22 Jun 1995 21:29:36 -0800
Subject: Re: Digital SEM imaging

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X-Sender: mager-at-pop.unixg.ubc.ca (Unverified)
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} I am interested in digitizing a JEOL 5300 SEM, Tungsten filament,
} with a passive system. This is nonbeam control. The SEM is
} in a core facility and both biological and materials are viewed. Has anyone
} up gradedan SEM with after market equipment and software? Are you happy
} with the results?

} Greg-at-umic.umic.sunysb.edu

Greg,
The only commercial passive SEM digital system I know of is the Quartz PCI
system. It is a Windows-PC system for acquiring, processing and archiving
images from the SEM. It normally stores images in TIFF format but can
export in about 20 formats. I have had one for about two years on a Hitachi
S-2300 (simple) SEM and I must say the darn thing is addictive. No-one who
has used it once will use the SEM without using it. My film use has dropped
to one-tenth, since most people in my multi-user university lab just use
the laserprinter prints. The slow-scan image (up to 2500 X 2000 resolution)
on the super-VGA screen is very striking. You can annotate and do line and
angle measurments on the image, then store, print or photo-replay. The
latest version has a full image database to keep track of all the images
and find them by searches on many different topics or keywords.
=7F
My lab was used for initial testing of this system on a real SEM and so I
have followed the progress of this product since its inception. The
installers say they can interface to any SEM and have also hooked up to my
S-570 (vertical picture) and H-800 STEM. I have been happy with the product
and the students all love the full Windows compatability. You can cut the
images to the clipboard then drop them into Word or any other program. Now
I sell discs to carry away the TIFF ore JPEG files instead of film. Also,
since my S-2300 does not have any annotation, I can annotate the Quartz PCI
image then re-spool it through the SEM's photo CRT. In fact, it turns your
SEM into a Polaroid printer for any image that the PCI system can import
(see 20 formats above).=7F
NSC Hitachi carries this in Canada and I think NSA Hitachi does in the US.
Hope this helps,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Eng, UBC
309 - 6350 Stores Rd.
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648, fax: 604-822-3619
email: mager-at-unixg.ubc.ca






From: Wu Yujing :      ih36-at-jove.acs.unt.edu
Date: Fri, 23 Jun 1995 00:27:37 -0500 (CDT)
Subject: unsubscribe

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unsubscribe





From: norenbur-at-onyx.si.edu (Jon L. Norenburg)
Date: Thu, 22 Jun 1995 11:36:49 -0500
Subject: Re: Legal/Ethical Questions

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Message-Id: {199506221536.LAA10282-at-onyx.si.edu}
Mime-Version: 1.0
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Dear folks,
I am surprised that there is not already an avalanche of responses to Dr.
Garber. In large measure I agree with him as concerns ethics and yes,
there are a lot of hypocritical facades out there. Whether or not his
brush is a little or a lot too broad I don't know, I have no direct
experience with "commercial" use of university labs. I do know of
colleagues who would not be running service labs, and whose labs would be
shut down without the modest commercial income that goes exclusively to
operating funds for the lab. Without that income they would not be
training any students and many of my colleagues who do non-sexy (i.e., no
or minimal public funding) morphological research in this day and age would
not be using electron microscopy as a tool.

I am very sensitive to Dr. Garber's concern about "fairness." But, as is
so common nowadays, some people like to see things as black and white - so
Dr. Gardner implies that there cannot be any possible justification for a
not-for-profit (which, by the way, also employ people who spend money at
stores, pay taxes, buy houses and raise kids that eat apple pie) accepting
commercial contracts. Even your elected congressional representative can
scam all the for-profit book and speaking engagements that their time and
importance in office garners for them. However, at the present rate of
evolution in Washington DC, the absolutist reasoning used by Dr. Garber may
soon dictate that all government functions be privatized, because they all
compete with someone or the potential of someone to provide a service for
profit (perhaps we can even replace the military with private militias). I
hope that he is planning on privately educating all those students who no
longer have labs and can no longer use government or commercial money to
buy his services or those of SPI.

Ethics enters into this argument only as concerns legalities and norms of
society; it has no position that says the private interests of an
entrepeneur override those of other individuals. If society insists on
certain laws (or if industry has bought them from equally ethical public
servants) - too bad, we have to live with them. If people find ways to
evade restrictions legally - too bad, we'll just have to live with
university research institutes. Yes, if a university enters into a
contract with NSF it should honor the terms of that contract. Once that
contract is no longer binding, I see no ethical problem, in principle, with
a university lab making ends meet through commercial contracts - I do see a
problem with the facilities being used inappropriately for personal gain IF
that is in violation of university regulations or civil law.

Entrepeneurs are not the only gamblers; loyalty to a public facility can be
just as much of a gamble - moreover, ethics has nothing to say about which
type of gamble is morally superior!

This note has been composed on personal time on a personally owned computer
with public domain software...
--Jon


Jon L. Norenburg {norenbur-at-onyx.si.edu}
Invertebrate Zoology - MRC 163
National Museum of Natural History
Smithsonian Institution, Washington, DC 20560
Voice 301-238-3508, Fax 301-238-3361






From: f705geri-at-mbox.tu-graz.ac.at
Date: Fri, 23 Jun 1995 08:53:12 +0200
Subject: Dye sub printers/Imaging

Contents Retrieved from Microscopy Listserver Archives
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We also have a Tektronix "Phaser II SDX" printing system connected to a
Mac 840AV with a Mac 17inch monitor. We are using Digital Micrograph for
image processing. The problem we have is that in many cases the colours
in the output on the printer differs significantly from the image on the screen.
Does any one known how to adjust/calibrate such a system ?

Geri


DI Gerald Kothleitner
Forschungsinst. f. Elektronenmikroskopie
Steyrergasse 17
8045 Graz, Austia





From: f705geri-at-mbox.tu-graz.ac.at
Date: Fri, 23 Jun 1995 09:51:59 +0200
Subject:

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We also have a Tektronix "Phaser II SDX" printing system connected to a
Mac 840AV with a Mac 17inch monitor. We are using Digital Micrograph for
image processing. The problem we have is that in many cases the colours
in the output on the printer differs significantly from the image on the screen.
Does any one known how to adjust/calibrate such a system ?

Geri


DI Gerald Kothleitner
Forschungsinst. f. Elektronenmikroskopie
Steyrergasse 17
8045 Graz, Austia





From: Baoming Huang :      bmhuang-at-sunchem.chem.uga.edu
Date: Fri, 23 Jun 1995 10:19:38 -0400 (EDT)
Subject: subscribe

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subscribe



* * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * *
Baoming M. Huang bmhuang-at-sunchem.chem.uga.edu
Department of Chemistry bmhuang-at-uga.cc.uga.edu
University of Georgia Tel: (706) 542-9453
Athens, GA 30602-2556 FAX: (706) 542-9454







From: Goldmarker-at-aol.com
Date: Fri, 23 Jun 1995 10:25:23 -0400
Subject: microbiology listserver?

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Does anyone know of the existence of a similar microbiology listserver
similar to Nestor's well-managed system?

This is a great group, but I need to talk to some environmental or
fermentation microbiologists about methods to quickly measure biomass
viability and to quickly id microbes.

My internet access is via America Online at the moment.

Any help would be appreciated.

Regards,

Donald P. Cox, Ph.D.
GOLDMARK BIOLOGICALS
437 Lock St
PHillipsburg, NJ 08865
(908) 859-2631
(98) 859-2875-FAX
E-mail: goldmarker-at-aol.com




From: bell-at-medcolpa.edu (Ted Bell)
Date: Fri, 23 Jun 1995 11:59:01 -0400
Subject: need to replace an outdated bioquant system

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We currently have an old bioquant system capable of flourescence and want to replace it with a state of the art system, which is not from Bioquant. First off, are there any recommendations, especially in terms of cameras and frame grabbers?

1) Currently, the computer is 33MHz Gateway 486? Is it worth replacing with a Pentium or second-generation PowerMac?

2) Which kind of camera is best, digital or analog? What the are the advantages and disadvantages of each?

3) If we go with an analog camera and frame grabber, does anyone know if any company is now offering PCI frame grabber cards for the second-generation Power Macintosh yet?






From: jeanne_barker-at-merck.com (Jeanne Barker)
Date: 23 Jun 1995 12:41:48 U
Subject: An atlas of the brain??

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I Have a Question regarding... An atlas of the brain??
6/23/95 11:46 AM
Hi,
I am beginning a new project involving morphology of the brain of the mouse,
particularly the cerebrum and the hippocampus. We will be doing transmission
E.M. My question is this--Can anybody recommend an atlas that shows T.E.M.
photographs of normal brain and perhaps some pathology?
Thank-you,
Jeanne






From: L. D. Marks
Date: 6/23/95 6:32 AM
Subject: Re: None

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Message-Id: {n1408220316.97921-at-macmail7.lbl.gov}

Reply to: RE} None

Laurie,

you could provide a link to
http://ncem.lbl.gov/NCEM/computer/software.html
for users to download NCEM's free software.

I think it makes more sense for us to keep the latest versions up on our server, rather than send you new versions as we make
changes (sometimes as often as daily).

Mike O'Keefe
Acting Head, NCEM
--------------------------------------

I am trying to collect programs for some of the more heavy-duty
aspects of electron microscopy, e.g. Multislice, Bloch-wave,
Image Processing (Maximum Entropy etc), Multibeam calculations,
Adaptive and Multifactor FFT's etc. I intend to place these on
our home page here at Northwestern, or I can include links to
other places where they can be found via anonymous ftp. All
this software will be Public Domain material, and I am primarily
thinking about Unix-based software with an X-windows based approach
to graphics so that it will run on different platforms.

Can you please pass this message on to one of your students
who can either send me directly via anonymous ftp any code that
you can donate or give me the appropriate link. You can email
me at ldm-at-apollo.numis.nwu.edu, but please use risc1.numis.nwu.edu
(129.105.122.66) for anonymous ftp (a different computer with
more space). The Web page link is
http://risc1.numis.nwu.edu/internet/lab.html
ftp://risc1.numis.nwu.edu

Thanks

Laurie Marks

P.S. Please pass this on to anyone else who you think might have
heavy-duty software that they are willing to contribute.






From: Dr Ross Mackenzie :      ross.mackenzie-at-Materials.oxford.ac.uk
Date: 23 Jun 1995 14:55:29 +0100
Subject: Lectureship in Materials

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"microscopy-at-aaem.amc.anl.gov" {microscopy-at-aaem.amc.anl.gov}
X-Mailer: Mail*Link PT/Internet 1.0.1

The following advertisement will appear in the scientific & educational press
in the near future.
Please draw it to the attention of appropriate colleagues.

Further information about this post and about the Oxford Materials Department
can be obtained from our WWW server. (http://www.materials.oxford.ac.uk/)

Ross Mackenzie

-----------------------------------------------------------------------

University of Oxford
Department of Materials in association with Trinity College or St Hilda's
College
University Lecturership in Materials

Applications are invited for the above post, tenable from 1 January 1996.
Stipend
according to age on the scale of £15154-£28215 per annum. The successful
candidate
may be offered a tutorial fellowship, for which additional emoluments would
be
available, by either Trinity College or St Hilda's College under
arrangements described
in the further particulars.

Candidates should be able to teach widely within a broad four-year Materials
Science
syllabus and to carry out a program of research into the mechanical
properties of
materials.

Further particulars (containing details of the duties and full range of
emoluments
and allowances attaching to both the university and college posts) may be
obtained from
The Head of Department, Department of Materials, Parks Road, Oxford OX1 3PH,
UK
(FAX 01865 273738, Tel: 01865 273737). The closing date for applications is
31 July
1995.

The University exists to promote excellence in education and research, and
is an equal
opportunities employer.

--------------------------------------------
Dr Ross Mackenzie
University of Oxford
Department of Materials
Parks Road, Oxford OX1 3PH

Phone 01865 273708 or 01865 273693
FAX 01865 273789
--------------------------------------------




From: hmeekes-at-biosci.mbp.missouri.edu (Herman Meekes)
Date: Fri, 23 Jun 1995 09:17:15 -0500
Subject: Re: SEM of fluoridated plastics

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On June 23 } Alex Titkov wrote:

} Dear Microscopists,
}
} We have a customer who wants to see the internal surfaces of light
} pipes made of various materials. The relevant features are about 100
} nm. The materials are fluoridated plastics (like teflon), but some of
} them are unstable even at 130C.
}
} We sputter coated the samples with gold (various thicknesses between
} 30 and 90 nm) and viewed them in a conventional SEM (W filament) at
} 15-25 kV keeping the beam current as low as possible (down to 30 pA).
}
} The problem is that the samples with thin coatings are unstable under
} the electron beam whereas thick coatings seem not to form a continuos
} layer of gold on the plastic, resulting in an orange peel appearance
} (200-300 nm dia. gold "islands"?).
}
} We would appreciate any ideas about imaging such samples.



I am not familiar with materials science, but I wonder if you could use
Pt/C replica techniques to solve the problem.

Briefly: expose the surface to be studied to a source of evaporating Pt in
high vacuum ( at a low angle). Stabilize by evaporating Carbon at a
perpendicular angle. Excise the parts of interest from your sample and free
the replica from the mould. View in TEM.
I seem to remember there even is a way of making an indirect replica by
first replicating the surface with a substance (resin?) and preparing the
Pt/C replica on that. Resolution in both instances should be in the 1-10 nm
range.

I think that more elaborate explanations of these techniques can be found
in EM manuals.




Herman Meekes
Biological Sciences ______________ ______________
University of Missouri ---__ \ / __---
109 Tucker Hall ------__\---/__------
Columbia, MO. 65211, USA \( )/
Tel: 314-882-0171 V
Fax: 314-882-0123 / \
e-mail: hmeekes-at-biosci.mbp.missouri.edu /___\






From: jpawley-at-macc.wisc.edu (James Pawley)
Date: Fri, 23 Jun 1995 12:14:17 -0600
Subject: Re: Legal/Ethical Questions

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Hear! Hear! to Jon Norenburg for taking the time to respond to some of the
points of Chuck Garber's diatribe. The thought of doing so wearied me that
I wanted to ignore it. Jon's example has woken me up!

He could have added that, at many respected institutions of higher learning
(Harvard, MIT ...), only about 50% of the prof's salaries are paid by the
institution and the prof is EXPECTED to garner the remaining 50% by
consulting. Are tuition rates not sufficiently high already? Pay them
less you say? How else does one get profs in technical fields to stay in
the university when they have to compete with manufacturers producing
military toys on "cost-plus" contracts? (Contracts, moreover, that are
increasingly used, in part, to pay EM service labs!)

Has Chuck heard about the almost total demise of the industrial microscopy
labs in the US? of the many 400kV, 300kV TEMs and VG-STEMs etc. given
away to anyone with a truck? Tax policies dictate this sort of shift but
someone still has to do the work, (unless we want it all done in Asia or
Europe). Why not the universities who have students to train and questions
to answer? The fact is that this system is often the most "cost effective"
way for a company to get an answer quickly and right. Like many economic
debates, it all depends on whatyou count as costs and what you are willing
to ignore to make the argument sound better.

We have heard a lot about NSF Doc. 91 but the microscopes in universities
and other non-profit organizations are not just bought using NSF funds.

We have heard about the Profs who just must replace their EMs every year or
two. I don't know where these profs are. Comprehensive surveys by MSA and
others show that the stock of microscopical instrumentation is aging and
deteriorating and that this has been the situation for some time... When I
sit on NSF study sections, the average age of the equipment to be replace
is 15 years (remember 1980? Reagan became President...)

The "bottom-line boy"s who now feel that they should run things
"efficiently" have cut spending on scientific infrastructure year after
year. Now, like the collapse of the school system in California, 20 years
after Prop. 13 "got gov't off their backs", the Universities are desparate
and are trying any old trick to keep afloat: tricks that, I may add, are
often heartily endorsed by the (other) for-profit enterpreneurs who now
hope to benefit from using university equipment and expertise that "they
have been paying for through their taxes for years"....

Everyone wants to cut the pie so that they get a little more and, it seems
to me, that this discussion is more about cupidity than anything else.
Ethics is an important topic, but, like wrapping oneself in the flag, there
is often a temptation to simplify it for the sake of making debating
points.

Not all things are well understood just by looking at the "Market Price".
After all, Chuck often contributes to our discussions in a helpful manner
but surely he doesn't suppose that his email connection fee actually covers
the cost of sending his thoughts to the hundreds of members of this list!

Jim Pawley

***************NEW ADDRESS**************
Prof. James B Pawley, Ph. 608-263-3147
Room 1235, Engineering Research Building, FAX 608-265-5315
1500 Johnson Dr. Madison, Wisconsin, 53706.
JPAWLEY-at-MACC.WISC.EDU






From: AWBlackwoo-at-aol.com
Date: Fri, 23 Jun 1995 14:38:16 -0400
Subject: SEM on Fluoridated Plastics

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In response to Alex Titkov's posting on this subject, there is no "secret" to
imaging this sort of feature except to keep on trying! A couple of tricks
ought to help, however.

First, forget about doing any low magnification imaging on the same sample,
cut away literally all of the material that isn't in the picture and silver
paint everything that isn't in the picture (you are welcome to guess which
brand I prefer). If the sample is not completely grounded, you don't have a
chance of success, no matter what instrument you are using.

Second, you may have some success with putting down a very thin layer of
carbon before gold coating. It's no cure, but it sometimes helps.

Third, cut away more material. If you need a gold layer as thick as the
features you're trying to image, you've lost half of the battle before you
started. When it's the inside of a tube, I generally find that the "real"
problem is that I'm trying to image down in the groove of a semicircular
section; you really only want a small segment of the circle for best results.

Finally, work quickly. The less time the sample spends under the beam, the
greater the chance of success. One of the perverse things about our
instruments is that we seem to see much more beam damage during the rapid
scanning while we frame the picture and focus, and much less during the slow
scan exposure of the photographic emulsion. You might be able to get away
with focusing on one area and then obtaining the micrograph from another by
cranking the stage over, but this generally gives adequate focus only at low
magnification, in my experience, at least.

Good luck with a tough assignment.

Andy Blackwood

Andrew W. Blackwood, Ph.D.
Structure Probe, Inc.
63 Unquowa Road
Fairfield, CT 06430-5015
Ph: 1 203 254 0000
FAX: 1 203 254 2262
e-mail: AWBlackwoo-at-aol.com





From: Michael OKeefe :      Michael_OKeefe-at-macmail7.lbl.gov
Date: 23 Jun 1995 12:17:46 U
Subject: Re: HREM Shareware

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Message-Id: {n1408212583.67569-at-macmail7.lbl.gov}

Reply to: RE} } HREM Shareware

L.D. Marks wrote --

} The purpose of my email was not an attempt to kill either
} EMMPDL or MASSL but completely different. The idea is to collect
} primarily X-based routines for HREM and other miscellaneous such as
} Semper code and libraries. I believe that there has been a disturbing
} trend over the last ten years for heavy-duty programs for HREM to
} be written on Government funding then sold commercialy. These } programs
} should be much easier and cheaper than they are (} $5000 in many cases).
} I intend to donate NUMIS to this (at least the multislice and imaging
} parts) as soon as I can rewrite for X-windows and thereby escape a
} licensing agreement.
} L.D. Marks

------------------------------------------------------

For a HREM image simulation program that was "written on Government funding" and so is (and has always been) available free, see --
http://ncem.lbl.gov/NCEM/computer/software.html

BTW, this program is not available on the Zaluzec EMMPDL because of the requirement that all programs there include source code. I
have insisted that NCEM institute a policy of not releasing source code after my sad experience with my simulation code SHRLI. Over
the years SHRLI was written with various government funding support (CSIRO in Australia, NSF in the U.S., and SERC in the U.K.) and
was sent out free until my boss in Cambridge found out that one of the individuals who had requested SHRLI was now advertising and
selling it under a different name (he had made a small change -- he had taken the four main programs and combined two of them to
produce a suite of three). Of course, then my boss insisted that we charge for SHRLI and use the income to augment our meager
travel funds.

Also BTW, I am a little worried at the sound of your expressed intent to "donate NUMIS . . . as soon as I can rewrite for X-windows
and thereby escape a licensing agreement". Some people might think that rewriting code to escape a contractural agreement is
getting close to piracy . . . .

Michael A. O'Keefe
Acting Head, NCEM






From: David Henriks :      73531.1344-at-compuserve.com
Date: 23 Jun 95 15:36:50 EDT
Subject: RF Interscience address

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Can anyone provide me with the address, TEL and FAX for RF Interscience?

Thank you!

David Henriks TEL: 800-728-2233 (toll-free in USA)
South Bay Technology, Inc. 714-492-2600
1120 Via Callejon FAX: 714-492-1499
San Clemente, CA 92673 USA e-mail: 73531.1344-at-compuserve.com





From: ldm2-at-apollo.numis.nwu.edu (L.D.Marks)
Date: Fri, 23 Jun 1995 14:50:25 -0500
Subject: Re: HREM Shareware

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Rewriting code simply to change it in a minor fashion is certainly not appropriate, and
probably not legal. Just to clarify....I am talking about major changes in about 50-80%
of the current code of NUMIS (which, except for Public Domain Material) I wrote.




From: Dwight Beebe :      beebed-at-ERE.UMontreal.CA
Date: Fri, 23 Jun 1995 15:38:52 -0400 (EDT)
Subject: TEM: KMnO4 fixation of plant cells

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Greetings!
I'm in kind of a bind. Does anyone have a protocol that they
would be willing to post directly to me regarding Mollenhauer's method of
KMnO4 fixation for plant cells? I'm interested only in a drastic method
for examining plant cell membranes and their continuity and I understand
Mollenhauer's protocol worked very well. Most texts and literature I
have direct access to consider this protocol outdated or too specialized
and so don't say much in practical terms about the how-to's.
Many thanks.

Dwight Beebe
Institut de recherche en biologie vegetale beebed-at-ere.umontreal.ca
Dept. de sciences biologiques Voice:514-872-4563
Universite de Montreal FAX:514-872-8496
4101, rue Sherbrooke est
Montreal, PQ H1X 2B2 Canada





From: Beverly E Maleeff :      Beverly_E_Maleeff%notes-at-sb.com
Date: 23 Jun 95 18:10:43 EDT
Subject: De-wrinkling thick sections

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Does anyone have a good method for removing very tiny wrinkles from half-micron
Epon-replacement sections?
My standard method, if chloroform stretching doesn't work, is to use sodium
ethoxide or methoxide etching. Unfortunately, in a sample I'm working on,
sodium ethoxide removed the cell components I'm studying (phospholipids) along
with the wrinkles. Are there any less destructive reagents out there?
TIA--

Bev Maleeff
SmithKline Beecham Pharmaceuticals
Toxicology-US, UE 0462
709 Swedeland Road
King of Prussia, PA 19406
610/270-7987
610/270-7202 fax
maleeffbe-at-sb.com or Beverly_E_Maleeff%Notes-at-sb.com





From: Louis M. Ross, Jr. :      geosclmr-at-showme.missouri.edu
Date: Sat, 24 Jun 1995 13:57:36 -0500
Subject: RE: Temp unsubscribe

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unsubscribe microscopy geosclmr-at-showme.missouri.edu





From: goran.alsterborg-at-mailbox.swipnet.se (JEOL Service)
Date: Sat, 24 Jun 1995 21:41:56 +0000
Subject: Re: Digital SEM imaging

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Message-Id: {199506241945.VAA04891-at-mailbox.swip.net}
X-Sender: m-13225-at-mailbox.swip.net (Unverified)
Mime-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: quoted-printable

} Dear Greg,

} If you wish to passively digitize the image from your JEOL JSM 5300, this=
=20
can } be done by the JEOL SemAfore. This is a low cost passive digitizer=20
listening
} to the signal being sent to the photo CRT. It digitizes with the same=20
} resolution as the slow scan display on the photo CRT (typically 1900 x=
1400
} pixels) and displays/stores the image as a file in BMP format.=20

} After getting the image into your PC, you can correct greylevels, zoom,=20
count } etc and get the image on paper either as a playback to the photo CRT=
=20
or as a
} print on a LaserJet, video or dye sublimation printer.

} The digitizing board goes into a PC (IBM compatible 486 or faster with min=
8
} Mbyte RAM) and the program operates under MS Windows. It certainly works=
on
} the 5300 and also on almost all old or new JEOL SEM=B4s.'

} This unit is presently marketed only in Europe and Asia, so for more
} information, please contact us directly (preferably not by e-mail as we=
just
} have started testing the net and do not yet have any routines).

} Best regards from Goeran Alsterborg

} JEOL(Skandinaviska)AB
} Vegagatan 19 Fax: +46 - 8 - 29 16 47
} 172 34 Sundbyberg =20
} SWEDEN Phone: +46 - 8 - 28 28 00





From: Kym Smith :      KYMSMITH-at-immuno.imvs.sa.gov.au
Date: Mon, 26 Jun 1995 09:15:05 GMT+1030
Subject: unsubscribe

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Message-Id: {9506252344.AA11941-at-huon.itd.adelaide.edu.au}

unsubscribe




From: D-PATTON-at-wpg.uwe.ac.uk
Date: Mon, 26 Jun 1995 10:14:38 +0000
Subject: LM MOLLUSCA CONCHIOLIN STAIN

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Message-Id: {sfee8eda.001-at-wpg.uwe.ac.uk}
X-Mailer: WordPerfect Office 4.0

Can anyone offer advice on the following problem?

The outer layer of molluscan shells (the periostracum) is a
proteinaceous sheet of conchiolin. After death the periostracum
rapidly decays. I wish to find a stain or reagent which will be
taken up by shells with a periostracum but rejected by shells
where the periostracum is lacking, (ie. a conchiolin-specific
stain or reagent.
Can anyone suggest a simple staining/reagent method for this??

From Karen Croker, C/O Dave Patton

(D-PATTON-at-uwe.ac.uk)
University of the West of England
Bristol
UK







From: KAKER-at-ctklj.ctk.si
Date: Mon, 26 Jun 1995 13:53:15 +0200 (WET-DST)
Subject: EDS intensity data

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Dear All,

Does anyone have a EDS intensity data for testing standardless programs.
Up to now we are collected with our SEM Jeol JSM 35-CF and EDS Edax
9100/40, 205 spectra from different samples, under the well known
conditions (tilt angle, take-off angle, accelerating voltage, detector
parameters and sample composition).

Best regards,

Henrik Kaker

Metal d.o.o.
SEM/EDS Laboratory
Koroska c.14
62390 Ravne
SLOVENIA

Tel: +386-602-21-131/5562
Fax: +386-602-20-436
Internet E-mail: Kaker-at-ctklj.ctk.si





From: rybicka-at-acsu.buffalo.edu
Date: Mon, 26 Jun 1995 12:42:47 -0400
Subject: TEM - GLYCOGEN ULTRASTRUCTURE

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Hi, All Those Interested in Glycogen,

The existing confusion in glycogen staining, its disappearance
after use of uranyl acetate en bloc, and other problems of glycogen in TEM
are explained in recent short review printed in MICROSCOPY TODAY, October,
1994, entitled
"GLYCOGEN GFRANULES REVISITED".

Glycogen in the tissue contains covalently bound protein. The
event is well established in biochemistry, but still misinterpreted in EM.
Since this protein contains enzymes involved in glycogen metabolism, the
structures composed of glycogen-protein complex were considered as cellular
organelles and called GLYCOSOMES in 1968 ! So called "glycogen granules"
stainable with uranium and lead represent protein component (enzymes)
attached to glycogen in glycosomes. Glycogen in these organelles appears
as small (3 nm) particles which form (20-30nm) aggregates. These particles
remain invisible after routine staining with uranium and lead salts because
glycogen, as a polymer of glucose, does not form ionic bonds.

Apparent removal of "glycogen granules" by uranyl acetate, or other
acids, used before tissue dehydration, is in fact the removal of protein
component. The bond of protein to glycogen is sensitive to the change in pH
(this sensitivity was used in the traditional purification of glycogen by
the treatment with strong alkali or acids). The soluble protein separated
from glycosomes by uranyl acetate is presumably washed out during
dehydration. Glycogen is not fixed per se, but it is stabilized in
glycosomes due to the fixation of the bound protein. After removal of
protein by acidic treatment the structure of glycosomes is destroyed, and
small, 3 nm, glycogen particles floate freely in the cell forming large
irregular clumps. In routinely stained tissue these clumps appear as
unstained spots often misinterpreted as a poor plastic polymerization.
However, histochemical staining (Thiery technique) shows that these spots
are composed of the clumps of glycogen particles.

First TEM study of the subject was: RYBICKA, K.1979. Virchows
Archiv B. Cell Pathol. 30, 355-47. The article included prior references
showing how the misinterpretation of glycogen in TEM appeared. Recent
references are listed in the mentioned review in MICROSCOPY TODAY. If you
cannot get it, please, let me know, and I will write them.

Considering present status of biochemical knowledge about
glycosomes,
microscopic and molecular biology techniques seem to offer a good field for
further research.

Good luck,

Krystyna Kielan Rybicka
SUNY at Buffalo
Physiology / Neurobiology
Buffalo, NY


---
-------------------------------------------------------------------------
email address rybicka-at-acsu.buffalo.edu
phone number (lab) 716 829 3575
-------------------------------------------------------------------------





From: Edmund Glaser :      eglaser-at-umabnet.ab.umd.edu
Date: Mon, 26 Jun 1995 13:22:49 -0400 (EDT)
Subject: Re: Legal/Ethical Questions

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Message-Id: {9506261654.AA23256-at-cid.infosel.com.mx}

Usually I am a passive observer to the transactions of this group. However,
James Pawley's recent bilious response to Charles Garber's thoughtful remarks
made me sit up and take notice. I found no "diatribe" there, although that is
perhaps in the eye of the beholder. While I am sure that Dr. Garber can
defend himself, Prof. Pawley's shotgun response missed a glaring target right
in front of him and he doesn't seem to know it exists. He mentions the fact
that many outstanding universities require their faculty to support their
salaries from their consulting (translation: grants) 50% and sometimes more.
Indeed, this is so and indeed, it was not always so. In the more than 30
years that I've been in academia, this trend of reducing hard money salary
and converting faculty into entrepreneurs renting space in their schools has
been accelerating.

Some consequences of this are apparent:
(1) No grant, no position. This is also a not very subtle attack at tenure.
What does it mean to have tenure if you can't put food on the table?
(2) The "respected" universities are being subsidized by those institutions
who do pay hard money salaries. A small calculation shows that a researcher
who puts 50% of his salary on the grant is charging the granting institution
that amount plus fringe benefits (about 30% of salary) plus overhead
(minimum 40%) on the two for each year. There's then that much less for
everyone else on the pay line, and fewer on the pay line.

The pressure is intense for all academic research institutions to participate
in the market oriented industrial research game. They don't know whether to
treat their faculty as employees or as independent entrepreneurs, but certainly
not as pure academics. Result: a "beat up on the faculty" policy. Make them
support themselves even it drags them into creative financing to help them
survive.

What's this got to do with the original topic? Simply this. Don't blame
problems on how to stay both solvent and employed on industry and
entrepreneurs who know the rules they work under and are trying to make
a profit. The fault is university administrations who, having
succumbed to the lure of big (nonprofit) money in research, are making up
rules as they go and are playing corporate games on the
backs of their faculties. That's what gets the latter into the ethical/moral
and legal morass that started this whole thing off. Since the university
administrations got this problem going, let them solve it, if they
know how. One obvious answer is to have universities stop trying to be
money making machines and go back to what they're supposed to do, be
institutions of learning. I don't think this is likely to happen soon.


Edmund Glaser, D. Eng.
Dept. Physiol.
Univ. Md. School. Med.
Baltimore, MD 21201 USA
Ph: (410) 706-5041
Fax: (410) 706-8341





From: Michael Cammer :      cammer-at-aecom.yu.edu
Date: Mon, 26 Jun 1995 15:06:13 -0400 (EDT)
Subject: vibration table need

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currently, we have floating vibration tables for our light microscopes.
We need one or two more and were wondering of cheaper alternatives, such
as tabletop units.

Any suggestions of vendors would be helpful.

Thank you--

Michael Cammer
cammer-at-aecom.yu.edu






From: ldm2-at-apollo.numis.nwu.edu (L.D.Marks)
Date: Mon, 26 Jun 1995 14:23:40 -0500
Subject: Ge Chemical Thinning

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Does anyone have a reasonable recipe for chemical thinning of Ge
which does not use Br (which has become a safety issue).

Thanks




From: Mike Bench :      bench-at-cems.umn.edu
Date: Mon, 26 Jun 1995 15:13:31 -0500
Subject: Re: Ge Chemical Thinning

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In message {199506261923.AA18751-at-apollo.numis.nwu.edu} L.D.Marks writes:
} Does anyone have a reasonable recipe for chemical thinning of Ge
} which does not use Br (which has become a safety issue).
}
} Thanks

Laurie,
The method I have used (it has been about 5 years since I have done this) for
both Ge and Si, which is also a safety concern, is a 9 to 1 solution of HNO3 and
HF at, I think, room temperature. I suspended dimpled samples into a beaker of
the solution using platinum tipped tweezers. I can't remember if I stirred the
solution during thinnig or not. Notwithstanding the safety concerns with HF, I
found it easier to work with than Br in methanol solutions (which I have used
for GaAs but not Ge). As I remember it the thinning is pretty rapid, with it
taking not more than a few minutes to perforate a 50 micron thick sample so it
is difficult to get very small holes and very small wedge angles. Otherwise the
sample quality was quite good.
Good luck,



Mike Bench
Dept. of Chem Eng & Mat. Sci
or CIE Microscopy Center
250 Amundson Hall Office: 612-625-8508
421 Washington Ave. S.E. Microscopy Center: 612-624-6590
Univ. of Minnesota Fax: 612-626-7246
MInneapolis, MN 55455





From: EvexAnalyt-at-aol.com
Date: Mon, 26 Jun 1995 17:19:11 -0400
Subject: Announce: Training Video for TN5500 available from Evex

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News Flash

Evex Analytical Instruments
(908) 874-3800

Evex has just produced a training Video for the Tracor Northern 5500 System

Future Videos to be released
Tracor Norther 2000
Noran Voyager
PGT IMIX
Kevex Delta




From: JOHNA-at-SCI.WFEB.EDU
Date: Mon, 26 Jun 1995 17:50:17 -0400 (EDT)
Subject: Re: TEM:diamond resharpening

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Also not to run down any companies; I have been quite pleased with
MicroStar's resharpening service on my old DuPonts.

John

___________________________________________________
| |
| John G. Aghajanian, Ph.D. |
| Worcester Foundation for Experimental Biology |
| 222 Maple Avenue |
| Shrewsbury, MA 01545-2737 |
| |
| Tel: 508 842-8921 ext. 147, 161 |
| Fax: 598 842-9632 |
| JOHNA-at-sci.wfeb.edu |
| |
|_________________________________________________|





From: masur-at-msvax.mssm.edu
Date: Mon, 26 Jun 1995 19:56:45 -0500
Subject: Wild M40 Tissue Culture Microscope camera adapter parts

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During a recent move, we have lost the trinocular assembly to attach a
Wild-modified Polaroid Land film adapter (which we still have)

The missing part(s)
#7567 phomicrographic attachment camera MKa2, fitting #3526 phototube
#3526 beamsplitter phototube


We will be delighted to purchase or barter for these parts

Lilly Ossowski, PhD
212-241-3194

or

Sandra K. Masur Box 1183
fax: 212-289-5945 Mount Sinai School of Medicine
phone: 212-241-6544 or 0089 NY NY 10029-6574
e-mail:masur-at-msvax.mssm.edu









From: David Henriks :      73531.1344-at-compuserve.com
Date: 27 Jun 95 12:22:35 EDT
Subject: Correction to Jet Thinning Germanium

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The recipe I gave in my last message was for jet sectioning of polycrystalline
germanium. While the solution is the same, the parameters for jet thinning
single crystal germanium are as follows:

The solution is designated BK-1 and in short is:
500ml methanol
100ml butyl cellosolve
90ml H2SO4
30ml HF
Temperature: -50C
Jet height: 4.5mm
Flow Rate: Medium
Volts: 40
Current: 20mA

I'm sorry for any confusion.

Best regards-

David Henriks TEL: 800-728-2233 (toll-free in USA)
South Bay Technology, Inc. 714-492-2600
1120 Via Callejon FAX: 714-492-1499
San Clemente, CA 92673 USA e-mail: 73531.1344-at-compuserve.com





From: Christine A Brantner :      cab-at-csd.uwm.edu
Date: Tue, 27 Jun 1995 12:57:39 -0500 (CDT)
Subject: LR White/Immunostaining

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Hi

I am a graduate student and I am trying to immunolocalize a protein to the
intracytoplasmic membranes of a bacteria.

Question?

Has anyone had experience infiltrating and embedding in LR White following
incomplete dehydration? Is 70% ETOH suffient? Does this improve the
retention of antigenicity for immunogold labeling? Does this protocol
require a modification of the polymerization schedule (time and/or
temperature)?

Thanks in advance. Any thoughts and comments would be helpful.

Chris Brantner

University of Wisconsin-Milwaukee
PO Box 413
Milwaukee, WI 53201

cab-at-csd.uwm.edu





From: GIDDINGS THOMAS H :      giddings-at-horton.Colorado.EDU
Date: Tue, 27 Jun 1995 09:18:31 -0600
Subject: ion milling

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I'm looking for advice on performance testing of an ion milling
device. A group here at Univ of Colorado is using a Gatan PIMS to
mill titanium shadowed S-layers from Sulfolobus. Of late, they have
not been able to reproduce the high resolution pattern in milled
samples. We have evaluated the S-layers by TEM of negative stained
and shadowed preps and by checking the diffraction patterns of both,
and we have not detected any problems with either the biological
sample or the titanium. We would like to determine whether the PIMS
is performing properly. Is there a standard sample that can be used
to evaluate ion milling? Or, are there tests that can be performed?
We are obviously novices in the field; any advice appreciated.

Thanks,

Tom Giddings
giddings-at-horton.colorado.edu





From: M.V. Parthasarathy :      mvp2-at-cornell.edu
Date: Tue, 27 Jun 1995 07:42:27 -0400 (EDT)
Subject: RE: vibration table need

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X-NUPop-Charset: English

In message Mon, 26 Jun 1995 15:06:13 -0400 (EDT),
Michael Cammer {cammer-at-aecom.yu.edu} writes:

} currently, we have floating vibration tables for our light microscopes.
} We need one or two more and were wondering of cheaper alternatives, such
} as tabletop units.Any suggestions of vendors would be helpful.
----
Partial list of table-top vibration isolation platform vendors & their Tel. Nos.:

Technical Maufacturing Corporation (TMC) 800-542-9725
Kinetic systems 800-992-2884
Barry Controls 617-787-1555
Newport Corp 714-253-1680
Herzan Vibration isolation systems (relatively economical models available)
714-859-7409

Good Luck.

*************************************************************************
M.V. Parthasarathy
Professor of Plant Biology & Director, EM Facility
Section of Plant Biology
228 Plant Science Building
Cornell University, Ithaca, NY 14850
Telephone: (607) 255-1734
Fax: (607) 255-5407
E-mail: mvp2-at-cornell.edu
***********************************************************************




From: wise-at-vaxa.cis.uwosh.edu
Date: Tue, 27 Jun 1995 13:11:30 +0000
Subject: Negative envelopes

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Does anyone have a source for white negative envelopes for TEM (3.5x4.5
inches) or SEM (4x5")? The only ones I can find are glassine or manila.

Bob Wise
Biology Department
UW Oshkosh
(414) 424-3404
wise-at-vaxa.cis.uwosh.edu






From: Goldmarker-at-aol.com
Date: Wed, 28 Jun 1995 06:23:43 -0400
Subject: Phalloidin Gold?

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Does anyone have a source of phalloidin gold - 15 or 20nm?

Regards,

Donald P. Cox, Ph.D.
GOLDMARK BIOLOGICALS
437 Lock St
Phillipsburg, NJ 08865
(908) 859-2631
(908) 859-2875-FAX
goldmarker-at-aol.com




From: Clint E. Young :      clintey-at-unixg.ubc.ca
Date: Tue, 27 Jun 1995 23:46:46 -0700 (PDT)
Subject: Quantitative Immunohistochemistry

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To fellow colleagues,

I am currently involved in trying to perform quantitative
immunohistochemistry using image analysis. Does anyone know of a
discussion group or mailing list on this topic?

Looking forward to your reply.

Clint Young
Department of Psychiatry
University of British Columbia






From: James R. Stets :      stetsjr-at-ttown.apci.com
Date: Wed, 28 Jun 1995 07:42:08 -0400 (EDT)
Subject: White negative envelopes

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Bob Wise asked about a source for white envelopes for TEM and SEM
negatives. In the past, we have obtained them through Ladd Research
Industries, Inc.

Ladd Research Industries, Inc.
P.O. Box 1005
Burlington, VT 05402
802-658-4961

I think they are still in business; my catalog is a few years old.

Jim Stets
Air Products and Chemicals, Inc.
Allentown, PA
"I speak only for myself"




From: bozzola-at-siu.edu (John. J. Bozzola)
Date: Tue, 27 Jun 1995 19:42:12 -0600
Subject: EM:micrographs for textbook

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We are revising a textbook on biological electron microscopy (SEM and TEM)
and are looking for interesting electron micrographs of biological
specimens. Especially we are interested in images of viruses, microwave
fixed specimens as compared to conventionally fixed specimens, plants, TEM
of fungi, insects and human norman tissue. Contact me directly if you have
such micrographs. Thank you in advance.

#############################################################################
John J. Bozzola, Director
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
#############################################################################






From: Katherine.S.Connolly-at-Dartmouth.EDU (Katherine S. Connolly)
Date: 28 Jun 95 13:14:42 EDT
Subject: negative envelopes

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Message-id: {17232611-at-dancer.Dartmouth.EDU}

Crimson Tech
325 Vassar St.
Cambridge, Mass 02139

Kate Connolly
Dartmouth Med School




From: chandler-at-lamar.ColoState.EDU (John Chandler)
Date: Wed, 28 Jun 1995 10:19:19 -0700
Subject: Re: Negative envelopes

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Message-Id: {v01520d01ac1739f11315-at-[129.82.126.28]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

} Does anyone have a source for white negative envelopes for TEM (3.5x4.5
} inches) or SEM (4x5")? The only ones I can find are glassine or manila.
}

I know that glassine envelopes are not OK for archival storage of
negatives, and manila are probably not either. Check some of your old
negatives that have been stored in them, and you'll likely find some
discoloration/darkening under the glued seam.

We now use transparent polyethylene negative storage envelopes from
Polysciences.

Cat. No. Neg. size latest price
07233 3.25in X 4in $29.70
07235 4in X 5in $32.30

In addition to providing better protection, you can look at the negatives
on a light table without taking them out of their envelopes.

--John






From: Lauri J. Pelliniemi :      ljpelmi-at-utu.fi
Date: Tue, 27 Jun 1995 08:00:51 -0900 (PDT)
Subject: Re: glycogen fixation

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Dear listreaders:
Glycogen is an interesting object, which is prone to cause problems
especially for unexperienced beginners. I have tried to explain parts of
the issue in: Pelliniemi et al.: Glycogen accumulations in
differentiating mesonephric ducts and tubuli in male human embryos. 1983
Anatomy and Embryology 168:445-453. A comparative table of glycogen
behaviour in different procedures is included.
Yours, Lauri
-----------------------------------------------------------------------------
I Dr. Lauri J. Pelliniemi I Telephone +358-21-633 7312 I
I Associate Professor I or +358-21-633 7209 I
I Laboratory of Electron Microscopy I I
I University of Turku I Telefax +358-21-633 7380 I
I Kiinamyllynkatu 10 I Internet LJPELMI-at-UTU.FI I
I FIN-20520 Turku I Bitnet/EARN LJPELMI-at-FIRIEN.BITNET I
I FINLAND, Europe I I
-----------------------------------------------------------------------------





From: David Henriks :      73531.1344-at-compuserve.com
Date: 27 Jun 95 12:14:45 EDT
Subject: Ge Chemical Thinning

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Laurie-

You may want to refer to the paper by Bernie Kestel "A Jet Electropolishing
solution for Silicon, Germanium, Tantalum, Niobium and Tungsten-Rhenium"
Ultramicroscopy 9 (1982) 379-384. If you prefer, I can send you a copy . It is
only 5 pages long so I could even FAX it if you need it fast. Just e-mail me or
give us a call and ask for a copy of Tech paper #5.

The solution is designated BK-1 and in short is:
500ml methanol
100ml butyl cellosolve
90ml H2SO4
30ml HF
Temperature: -60C
Jet height: 6.5mm
Flow Rate: Fast
Volts: 150
Current: 95mA

Kestel writes:

"The small amount of HF in the BK-1 removes thin oxides well, yet creates only a
moderate hazard. The butyl cellosolve widens the voltage plateau for optimal
polishing and improves the finish obtained across precipitates and grain
boundries "
This work was done with the Model 550 Single Vertical jet electropolisher.
Voltage, temp etc. will have to be adjusted if you are using a twin jet system.

I hope this helps!

Best regards-

David Henriks TEL: 800-728-2233 (toll-free in USA)
South Bay Technology, Inc. 714-492-2600
1120 Via Callejon FAX: 714-492-1499
San Clemente, CA 92673 USA e-mail: 73531.1344-at-compuserve.com





From: ldm2-at-apollo.numis.nwu.edu (L.D.Marks)
Date: Wed, 28 Jun 1995 13:45:42 -0500
Subject: Re: Ge Chemical Thinning

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With thanks to various people for recipes on Ge thinning, I still have
one caveat and one question.
The caveat is that we are going to use the Ge samples in our UHV microscope.
As a consequence large hydrocarbon molecules such as butyl cellosolve are
the last thing that we want -- it may take forever to remove these to obtain
clean surfaces.
The question concerns what chemical for what crystallographic orientations?
We have found experimentaly that the standard Si polish works well for Ge
(001) but fails for Ge (111). Does anyone know of recipes for Ge (111) ?




From: John W Heckman :      heckman-at-pilot.msu.edu
Date: Tue, 27 Jun 1995 20:12:58 -0400 (EDT)
Subject: Re: Color Correction

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Message-Id: {9506280012.AA15582-at-pilot01.cl.msu.edu}

Damian:

The color correction you depends on the film type you are using as
well as the output freqs. of the light source. For Kodak Ektachrome and
Kodachrome 64's 30M +2/3 stop is recommended for average fluorescent light.
Exact correction depends on just what type of tubes are in the light (and their
age too I'd suspect). For more info, might check Kodak Pub E-77 or the latest
update thereof (If you are still in a jam I can Fax the table).

cheers,
John Heckman
heckman-at-pilot.msu.edu
} } } Good
Morning Everyone }
} I know this is supposed to be a microscopy forum, BUT, I'm in a jam and need
} to know
} what CC filters to use to correct for standard fluorescent lamps using
} daylight film. I had
} a sheet that listed the different filtration packs for different lamps but
} can't find it. I have
} a wide selection of large CC filters, know that the correction is mostly a
} magenta filter but
} also remember that other CC colors were used. No time to hunt down a 72 mm
} FLD filter!
}
} Many thanks to anyone who can help.
}
} Damian
} neuberd-at-roundlake.baxter.com
}





From: JOHNA-at-SCI.WFEB.EDU
Date: Wed, 28 Jun 1995 09:42:39 -0400 (EDT)
Subject: Re: LR White/Immunostaining

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Hello Chris,

Yes, LR White is great stuff for IMC. DO NOT use Osmium as per their
instruction sheet. Dehydration through 70% EtOH is sufficient and you can
dehydrate through absolute EtOH. You probably should test both to see how
your antigen reacts. If you don't have complete polymerization after
overnite and 50-55 C, leave the blocks at 60 C for a day (I try to avoid
the latter, however.). Good luck.

John

___________________________________________________
| |
| John G. Aghajanian, Ph.D. |
| Worcester Foundation for Experimental Biology |
| 222 Maple Avenue |
| Shrewsbury, MA 01545-2737 |
| |
| Tel: 508 842-8921 ext. 147, 161 |
| Fax: 598 842-9632 |
| JOHNA-at-sci.wfeb.edu |
| |
|_________________________________________________|





From: braunfeld-at-msg.ucsf.edu
Date: Wed, 28 Jun 1995 12:15:44 -0700
Subject: EPMA Beam deflection

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Message-Id: {9506281056.AA52602-at-pukrs7.puk.ac.za}

Has anyone out there in the cryo world heard of an Italian company that
makes cold stages which are supposed to be a less expensive alternative
to either gatan or oxford??? I've heard mention of one but do not know
who they are ar how to contact them. If anybody knows something please
let me know. thank you.




From: Brian J. Zande :      bz0c+-at-andrew.cmu.edu
Date: Wed, 28 Jun 1995 16:30:57 -0400 (EDT)
Subject: unsuscribe

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Message-ID: {IjwPk1C00iV9Q6VAIf-at-andrew.cmu.edu}


please unsuscribe




From: DRStadden:R_D:Armstrong
Date: 6-28-95 3:30pm
Subject: OM Polymer Staining

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To: MICROSCO:CENTRAL
cc: DRStadden:R_D:Armstrong
Subj: OM Polymer Staining
------------------------------------------------------------------

I'm wondering if there may be a good course one might recommend in the
subject area of staining methods for optical microscopy of polymers. I
know the subject has been dealt with in various texts, but can't say
that I know of any formal educational opportunities in this area.
Also, if it included stains useful to SEM of polymer composites, that
would be a plus. My experience is with PLM and SEM of flooring and
ceiling products, gasketing and felt products, and rubber insulation and
textile mill products, as well as components thereof. Thanks in advance
for any suggestions.

Dave Stadden
DRStadde+aCENTRAL%ARMSTRONG-at-MCIMAIL.COM

Ph: 717-396-5109
FAX: 717-396-5865





From: Tamara A Howard :      howie+-at-pitt.edu
Date: Wed, 28 Jun 1995 15:52:51 -0400 (EDT)
Subject: TEM tech position open

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Message-Id: {9506282122.AA07137-at-worldlink.worldlink.com}


TEM Technician Position Open

The Department of Pathology's EM Research Facility is seeking qualified
applicants for an EM technician position; a background in cell biology is
preferred. The applicant should have experience in specimen preparation
for TEM, particularly in ultrathin sectioning a wide variety of
specimens. Experience in EM autoradiography and immunocytochemistry are
not required but would be extremely helpful. The person in this position
will be responsible for handling research EM projects for academic and
clinical pathologists, post-docs, residents, and fellows.
Salary commensurate with experience.
The University of Pittsburgh is an Equal Opportunity Employer
Contact:
Fang He
S-704 Scaife
Cell/Molecular Pathology
3550 Terrace St.
U. Pittsburgh School of Medicine
Pittsburgh, PA 15261
(412) 648-9467
fan-at-med.pitt.edu




From: dennbarr-at-emn.com (Dennis B. Barr)
Date: Tue, 27 Jun 1995 08:49:34 -0400
Subject: RE Vibration table need

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I recently saw a description of a vibration-free platform for a table top from
Terra Universal, Inc., 700 N. Harbor Blvd., Anaheim, CA 92805.

Our lab once used a couple of table-top units from another manufacturer (who is
no longer in business). The pneumatic isolators in those units effectively
reduced vibration to acceptable levels.

Among other possible sources there are Kinetic Systems, Inc., 20 Arboretum
Road, P.O. box 414, Boston, MA 02131, and Technical Manufacturing Corporation,
15 Centennial Drive, Peabody, MA 01960.
Dr. Dennis B. Barr
Eastman Chemical Company
Microscopy and Morphology Research Laboratory
Kingsport, TN 37662-5150

Phone: 615/229-2188
E-mail: dennbarr-at-emn.com
FAX: 615/229-4558






From: kennedy-at-nsi.edu (grace kennedy)
Date: Wed, 28 Jun 1995 15:28:01 -0800
Subject: Lr White IM

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X-Sender: grace-at-198.147.151.5
Message-Id: {ac17943f02021004e631-at-[198.147.151.19]}
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In addition to John Aghajanian's recommendations, I would suggest the
following two papers: "An Osmium-free Method of Epon Embedment That
Preserves both Untrastructure and Antigenicity for Post-embedding
Immunocytochemistry", by Phen, K.D., Rustioni, A., and Weinberg, R.J., J.
Histochem.Cytochem. 42, 1995 This method should adapt nicely to LR White.
Sorry I don't have page numbers. And, "An Enhanced Method for
Post-embedding Immunocytochemical Staining Which Preserves Cell Membranes",
by Berryman, M.A., and Rodewald, R.D., J. Histochem. Cytochem. 38 (2)
159-170, 1990. Also would adapt nicely to LR White material. Grace
Kennedy






From: Steven Bagley :      SBAGLEY-at-fs2.scg.man.ac.uk
Date: Tue, 27 Jun 1995 16:35:00 GMT+1
Subject: vibration tables

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} currently, we have floating vibration tables for our light microscopes.
} We need one or two more and were wondering of cheaper alternatives, such
} as tabletop units.
}
Hi Michael,

A idea I have used for a confocal microscope is using a concrete
table. On top of the table a slab on slate or granite - from your
friendly local stone mason. Sandwiched between the two are four heavy
duty inner tubes with special valves which do not allow air out when
the tubes are pumped up.

In the sandwich layer you may also wish to put four wooden blocks
thus the table can be leveled by using these blocks as a reference.
This may sound a 'wacky' set up but this table has been in use four
five years with no problems yet.

Hope this has helped

regards,


Steve Bagley

__________________________________________________________________

The Confocal Microscope Facility
Cells, Immunology and Development Voice: 0161 275 6771
University of Manchester Fax: 0161 275 3915

http://gonzo.sci.man.ac.uk/~sbagley/

If you tied buttered toast to the back of a cat and
dropped it from a height, what would happen ?
___________________________________________________________________




From: richard.easingwood-at-stonebow.otago.ac.nz (Richard Easingwood)
Date: Wed, 28 Jun 1995 11:24:02 +1100
Subject: Critical Point Dryer/Dye Sub Printers

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X-Sender: st004718-at-brandywine.otago.ac.nz
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Thanks very much for all those who were kind enough to write regarding
their experiences with their dye sublimation printers. There are very few
of these in NZ and its reassuring to get feedback from users.

On a different note, we are seriously looking at buying a Balzers CPD 030
critical point dryer and again would be grateful to hear from anyone who
has one as to whether there is anything we should be aware of with regards
to its use (or for that matter its accessories). We had our fingers burnt
three years ago when the university bought two new critical point dryers
(NOT Balzers) which turned out to be extremely unreliable and had to have
extensive and expensive modifications done to make them useable, even so
they have never really been satisfactory. We want to avoid a repeat of that
experience at all costs as we are very lucky to get funding again for a
replacement. I'm sure the Balzers instrument is in a different (better)
league altogether but if anyone has any good or bad stories with the CPD
030, I'd like to hear.
Regards and thanks,

Richard

Richard Easingwood
South Campus Electron Microscope Unit
Otago Medical School
PO Box 913
Dunedin
NEW ZEALAND

Telephone: 64-03-479 7301
Facsimile: 64-03-479 7254

"The southernmost electron microscope unit in the world"






From: chandler
Date: Wednesday, June 28, 1995 10:19AM
Subject: Re: Negative envelopes

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} Does anyone have a source for white negative envelopes for TEM (3.5x4.5
} inches) or SEM (4x5")? The only ones I can find are glassine or manila.
}

I know that glassine envelopes are not OK for archival storage of
negatives, and manila are probably not either. Check some of your old
negatives that have been stored in them, and you'll likely find some
discoloration/darkening under the glued seam.

We now use transparent polyethylene negative storage envelopes from
Polysciences.

Cat. No. Neg. size latest price
07233 3.25in X 4in $29.70
07235 4in X 5in $32.30

In addition to providing better protection, you can look at the negatives
on a light table without taking them out of their envelopes.

--John






From: shirley.turner-at-NIST.GOV (Shirley Turner)
Date: Wed, 28 Jun 1995 13:14:35 -0400 (EDT)
Subject: ED: NIST Workshop on Crystallographic Databases

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Members of the electron diffraction community that are interested in using
crystallographic databases in materials characterization are invited to
attend the 'NIST Workshop on Crystallographic Databases' to be held on
August 29-30, 1995 at the National Institute of Standards and Technology. A
main goal of the Workshop is to foster interactions between users and
providers of crystallographic databases and between the communities that use
the different databases. Three sessions - each with seven or more
presentations- will be held and are summarized below:

(1) Formal Data Activities; Chair: David Watson, Cambridge
This session will focus on discussion of present activities and future
activities associated with the following data centers:

The Metals Database
Inorganic Crystal Structure Database (ICSD)
The Protein Data Bank
NDB- Nucleic Acid Database
The Cambridge Structural Database
The Powder Diffraction File
NIST Crystal and Electron Diffraction Data Center

(2) Scientific Uses of the Databases; Chair: Carolyn Brock, Univ. of Kentucky
This session will focus on using crystallographic databases in analysis, in
the prediction of materials properties and in the design of new chemicals,
pharmaceuticals and materials.

(3) Data Transfer: Ensuring state-of-the-art technology; Chair: Brian
McMahon, IUCr
This session will focus on issues related to data transfer such as:

(1) data exchange standards (CIF, etc.),
(2) the role of journals in the evaluation of published data;
(3) data exchange between the journals and crystallographic data
centers;
(4) computerized modes of data dissemination.

Following the presentations, two discussion sessions will focus on Barriers
to the Use of Crystallographic Data and on Partnerships for the Future.
Workshop proceedings will be published in a special issue of the NIST
Journal of Research. There is no registration fee and attendance will be
limited in order to hold the Workshop to an efficient and practical size.
Please contact us if you would like further information or registration forms.

Dr. Vicky Lynn Karen karen-at-tiber.nist.gov
Dr. Alan D. Mighell mighela-at-tiber.nist.gov
NIST Crystal and Electron Tel: 301-975-6255
Diffraction Data Center Fax: 301-975-2128
Materials Building, Room A215
Nat'l. Inst. of Stds. & Tech.
Gaithersburg, Maryland 20899





From: GVKM07A-at-prodigy.com (DR CHARLES A GARBER)
Date: Tue, 27 Jun 1995 20:28:05 EDT
Subject: Different legal issue

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-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --

I have received in my mail box so far a total of 31 "private" replies
to my postings of a week ago in response to John Wheatley's original
posting. This subject clearly strikes the nerves of more than a few
who follow this listserver.

One especially interesting issue was raised in several of these private
notes and it had to do with the "legal" side of the discussion but from
a different perspective: Can a client sue a university faculty member
(or the university itself) if the client thinks the university made a
mistake and caused the client economic loss?

I suspect that this will come as a surprise to some (many?) but the
answer is very definitely "yes!".

The US court system has made it very clear that when someone holds
themselves out as offering a professional service, be it a faculty
member offering electron microscopy services or a neurosurgeon doing
brain surgery, then the public has a right to expect a minimum level of
proficiency and competence and when the public perceives they did not
get that minimum level, then under the present laws and court system,
the injured party has a right to seek some relief through that court
system, and to recover damages they might have suffered as a
consequence. This kind of exposure is called "professional risk" and
one protects their assets by purchasing professional liability
insurance.

I have not verified the statistics, but I have been told by one person
who has looked at this that someone "consulting" is more likely to get
caught up in some kind of a professional liability action than he is
in his life time to run down and kill someone with his car. Yet would a
responsible person ever dream of driving their car for even one minute
without automobile insurance?

Among the independent analytical and testing laboratory community,
there is unanimous recognition of the need to have professional
liability insurance coverage. No reputable and financially responsible
laboratory would be without such coverage. Laboratories do get sued on
a regular basis. I am also including microscopy laboratories in that
statement. Such actions might not make CNN or Peter Jennings, but they
do occur on a regular basis. One can spend $100,000 without any effort
at all on a typical case in federal court, and even if the judge
ultimately dismisses the "consultant" from the case, there is still
nevertheless the obligation for the payment of the legal fees and
share of the court costs.

No university, public or private, has been granted any kind of
immunity from such suits. So the point is that in order to do this
"commercial" work, in order to bring in this extra money, despite the
potential for disruption to legitimate academic programs and also to
the commercial marketplace, this activity puts the entire university at
risk in ways that are never even considered. And for a public
institution, the real losers are the taxpayers, who now have to carry
the buden or risk of the folly of the commercial activities being
conducted out of the university's laboratories.

Chuck

======================================================
Charles A. Garber, Ph. D.
President
SPI SUPPLIES Div. of Structure Probe, Inc.
PO BOX 656
West Chester, PA 19381-0656 USA

Ph: 1-(610)-436-5400
1-(800)-2424-SPI

FAX: 1-(610)-436-5755

e-mail: GVKM07A-at-prodigy.com
SpiSupp-at-aol.com [SPI Customer Service e-mail box]
======================================================





From: Clint E. Young :      clintey-at-unixg.ubc.ca
Date: Wed, 28 Jun 1995 17:17:40 -0700 (PDT)
Subject: Agar bound to slides

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To fellow colleagues,

Can anyone suggest how I can make chunks of agar adhere to slides? I've
tried using poly-lysine coated slides and saline coated slides, but both
require that the agar be DRIED onto the slides--something I want to avoid.

Looking forward to your reply.

Clint Young
Department of Psychiatry
University of British Columbia




From: fr.Bob Roberts i.n.d.c. :      bobrob-at-cam.org
Date: Sat, 24 Jun 1995 08:52:46 -0400
Subject: Re: (Fwd) A little English humor for the week-end (long_

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Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-isnet.is.wfu.edu *
**************************************************************

---------- Forwarded message ----------

Hi everybody
Hi everybody
Summer is here and some of you might be travelling.
And this can happen to you! ;-)

} *********************************************************************
}
}
} Subj: Hotel soap
} Date: Wed, Jun 21, 1995 12:13 AM EDT
}
} RE: Hotel soap
}
} Attached is some correspondence which actually occurred between a
} London hotel's staff and one of its guests. The London hotel
} involved submitted this to the Sunday Times. No name was mentioned.
}
} ******************************************************************************
} Dear Maid,
} Please do not leave any more of those little bars of soap in my
} bathroom since I have brought my own bath-sized Dial. Please remove
} the six unopened little bars from the shelf under the medicine chest
} and another three in the shower soap dish. They are in my way. Thank
} you,
} S. Berman
} ----------------------------------------------------------------------
} Dear Room 635,
} I am not your regular maid. She will be back tomorrow, Thursday,
} from her day off. I took the 3 hotel soaps out of the shower soap
} dish as you requested. The 6 bars on your shelf I took out of your
} way and put on top of your Kleenex dispenser in case you should
} change your mind. This leaves only the 3 bars I left today which my
} instructions from the management is to leave 3 soaps daily.
} I hope this is satisfactory.
} Kathy, Relief Maid
} ----------------------------------------------------------------------
} Dear Maid -- I hope you are my regular maid.
} Apparently Kathy did not tell you about my note to her concerning the
} little bars of soap. When I got back to my room this evening I found
} you had added 3 little Camays to the shelf under my medicine cabinet.
} I am going to be here in the hotel for two weeks and have brought my
} own bath-size Dial so I won't need those 6 little Camays which are on
} the shelf. They are in my way when shaving, brushing teeth, etc.
} Please remove them.
} S. Berman
} ----------------------------------------------------------------------
} Dear Mr. Berman,
} My day off was last Wed. so the relief maid left 3 hotel soaps which
} we are instructed by the management. I took the 6 soaps which were
} in your way on the shelf and put them in the soap dish where your
} Dial was. I put the Dial in the medicine cabinet for your
} convenience. I didn't remove the 3 complimentary soaps which are
} always placed inside the medicine cabinet for all new check-ins and
} which you did not object to when you checked in last Monday. Please
} let me know if I can of further assistance.
} Your regular maid,
} Dotty
} ----------------------------------------------------------------------
}
}
}
} Dear Mr. Berman,
} The assistant manager, Mr. Kensedder, informed me this A.M. that you
} called him last evening and said you were unhappy with your maid
} service. I have assigned a new girl to your room. I hope you will
} accept my apologies for any past inconvenience. If you have any
} future complaints please contact me so I can give it my personal
} attention. Call extension 1108 between 8AM and 5PM. Thank you.
} Elaine Carmen
} Housekeeper
}
} ----------------------------------------------------------------------
} Dear Miss Carmen,
} It is impossible to contact you by phone since I leave the hotel for
} business at 745 AM and don't get back before 530 or 6PM. That's the
} reason I called Mr. Kensedder last night. You were already off duty.
} I only asked Mr. Kensedder if he could do anything about those little
} bars of soap. The new maid you assigned me must have thought I was a
} new check-in today, since she left another 3 bars of hotel soap in my
} medicine cabinet along with her regular delivery of 3 bars on the
} bath-room shelf. In just 5 days here I have accumulated 24 little
} bars of soap. Why are you doing this to me?
} S. Berman
}
} ----------------------------------------------------------------------
} Dear Mr. Berman,
} Your maid, Kathy, has been instructed to stop delivering soap to your
} room and remove the extra soaps. If I can be of further assistance,
} please call extension 1108 between 8AM and 5PM. Thank you,
} Elaine Carmen,
} Housekeeper
}
} ----------------------------------------------------------------------
} Dear Mr. Kensedder,
} My bath-size Dial is missing. Every bar of soap was taken from my
} room including my own bath-size Dial. I came in late last night and
} had to call the bellhop to bring me 4 little Cashmere Bouquets.
} S. Berman
}
} ----------------------------------------------------------------------
} Dear Mr. Berman,
} I have informed our housekeeper, Elaine Carmen, of your soap problem.
} I cannot understand why there was no soap in your room since our
} maids are instructed to leave 3 bars of soap each time they service a
} room. The situation will be rectified immediately. Please accept my
} apologies for the inconvenience.
} Martin L. Kensedder
} Assistant Manager
}
} ----------------------------------------------------------------------
}
}
} Dear Mrs. Carmen,
} Who the hell left 54 little bars of Camay in my room? I came in last
} night and found 54 little bars of soap. I don't want 54 little bars
} of Camay. I want my one damn bar of bath-size Dial. Do you realize
} I have 54 bars of soap in here. All I want is my bath size Dial.
} Please give me back my bath-size Dial.
} S. Berman
}
} ----------------------------------------------------------------------
} Dear Mr. Berman,
} You complained of too much soap in your room so I had them removed.
} Then you complained to Mr. Kensedder that all your soap was missing
} so I personally returned them. The 24 Camays which had been taken
} and the 3 Camays you are supposed to receive daily (sic). I don't
} know anything about the 4 Cashmere Bouquets. Obviously your maid,
} Kathy, did not know I had returned your soaps so she also brought 24
} Camays plus the 3 daily Camays. I don't know where you got the idea
} this hotel issues bath-size Dial. I was able to locate some
} bath-size Ivory which I left in your room.
} Elaine Carmen
} Housekeeper
} ----------------------------------------------------------------------
} Dear Mrs. Carmen,
} Just a short note to bring you up-to-date on my latest soap
} inventory.
}
} As of today I possess:
}
} - On shelf under medicine cabinet - 18 Camay in 4 stacks of
} 4 and 1 stack of 2.
} - On Kleenex dispenser - 11 Camay in 2 stacks of 4 and 1
} stack of 3.
} - On bedroom dresser - 1 stack of 3 Cashmere Bouquet, 1
} stack of 4 hotel-size Ivory, and 8 Camay in 2 stacks of 4.
} - Inside medicine cabinet - 14 Camay in 3 stacks of 4 and 1
} stack of 2.
} - In shower soap dish - 6 Camay, very moist.
} - On northeast corner of tub - 1 Cashmere Bouquet, slightly used.
} - On northwest corner of tub - 6 Camays in 2 stacks of 3.
}
} Please ask Kathy when she services my room to make sure the stacks
} are neatly piled and dusted. Also, please advise her that stacks of
} more than 4 have a tendency to tip. May I suggest that my bedroom
} window sill is not in use and will make an excellent spot for future
} soap deliveries. One more item, I have purchased another bar of
} bath-sized Dial which I am keeping in the hotel vault in order to
} avoid further misunderstandings.
} S. Berman
}
} ---------------------------------------------------------
} "Water is composed of two gins, Oxygin and Hydrogin. Oxygin is pure
} gin. Hydrogin is gin and water."
}

Soon Y. Choi

"What is history but a fable agreed upon?" - Napoleon B.



| /\_/\
| (- -)
\_-------=\"/= email: bobrob-at-cam.org
| ______ / URL: http://www.cam.org/~bobrob/
|| ||
|| || God Bless you one and all fr. Bob+ {8-))
^^ ^^














From: Dr P. Echlin :      pe13-at-cus.cam.ac.uk
Date: Thu, 29 Jun 1995 07:58:04 +0100 (BST)
Subject: Re: your mail

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I reply to the request about Italian Companies who might make inexpensive
cold stages, you might try contacting Prof. Ugo Valdre, Department of
Physics, University of Bologna, Bologna, Italy. He has made cold stages
in the past and may well know of the contacts in Italy

\Good luck in your search

Patrick Echlin
Director, Multi-Imaging Centre
University of Cambridge
On Wed, 28 Jun 1995
braunfeld-at-msg.ucsf.edu wrote:

} Has anyone out there in the cryo world heard of an Italian company that
} makes cold stages which are supposed to be a less expensive alternative
} to either gatan or oxford??? I've heard mention of one but do not know
} who they are ar how to contact them. If anybody knows something please
} let me know. thank you.
}




From: Dr P. Echlin :      pe13-at-cus.cam.ac.uk
Date: Thu, 29 Jun 1995 08:07:10 +0100 (BST)
Subject: Re: (Fwd) A little English humor for the week-end (long_ (fwd)

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The person who is so concerned about hotel soap surely must have been
staying at Fawlty Towers !!

Patrick Echlin




From: Romuald Wroblewski onkpat :      Romuald.Wroblewski-at-onkpat.ki.se
Date: Thu, 29 Jun 1995 09:43:13 +0200 (METDST)
Subject: unsubscribe

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Unsubscribe (for the summer)
I will be back in September.
Thanks for the humor in latest messages from London hotel. I loved it
although it is not directly forum for this type of messages but from time
to time it is OK.

-------------------------
Romuald Wroblewski, Ph.D.
Associate Professor
Department of Pathology
Karolinska Institute
Stockholm, Sweden
voice:+46-8-7293597
-------------------------





From: digigene-at-pavilion.co.uk (Dr L Van der Pant)
Date: Thu, 29 Jun 1995 08:55:56 +0100
Subject: DIGITAL TEM CCD OR FILM

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Dear All,
I have been listening with a great deal of interest the debate about the
pros and cons of digital imaging. The company that I work for manufacture
cooled CCD cameras and interfaces for TEM's. In my experience the following
is true.
1. For biological samples at low magnification you lose image definition,
i.e. it becomes impossible to define a fine structure (membrane typically) at a
precise mag. i.e. at 2K you can see it at 1K you can't.Therefore it is
possible for
some simple teste on a given sample to determine when exactly the camera
system "loses it". Work on this basis
with a 1,000x1,000 pixel camera at the low end magnification would seem to
indicate that 2,000x2,000 pixel cameras will make the whole thing a lot more
viable.
2. Digital cameras give much better results than conventional TV.
3. YAG scintillant provides a consistant image in terms of uniformity of
illumination with no low structure (granularity) present even with high dynamic
imaging to 12-bits.

The other preferences dicussed really depend on whether your primary
interest is "the image" or results from the image i.e. gold labelleing
analysis. On this basis most people
tend to live with the CCD and forego the joys of the dark room.

As far as the future goes 2Kx2K cameras are here, but are very expensive.
Cost effective options will be available with 2Kx2K sensors, but probably
with column defects. But if they are reproducible then they can generally be
removed in the computer.


Leslie Vanderpant
DIGITAL PIXEL LIMITED
PO BOX 625
BRIGHTON BN1 5JT
UNITED KINGDOM

t) 00 44 1273 502176
f) 00 44 1273 502176





From: zvert-at-r1.atki.kfki.hu (Zofia Vertesy)
Date: Thu, 29 Jun 1995 10:03:46 +0200
Subject:

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From: Rolf Odselius :      Rolf.Odselius-at-emu.lu.se
Date: Thu, 29 Jun 1995 14:27:40 +0100
Subject: Re: Critical Point Dryer

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Richard Easingwood wrote:
} ...................we are seriously looking at buying a Balzers CPD 030
} critical point dryer and again would be grateful to hear from anyone who
} has one as to whether there is anything we should be aware of with regards
} to its use (or for that matter its accessories). We had our fingers burnt
} three years ago when the university bought two new critical point dryers
} (NOT Balzers) which turned out to be extremely unreliable and had to have
} extensive and expensive modifications done to make them useable, even so
} they have never really been satisfactory. We want to avoid a repeat of that
} experience at all costs as we are very lucky to get funding again for a
} replacement. I'm sure the Balzers instrument is in a different (better)
} league altogether but if anyone has any good or bad stories with the CPD
} 030, I'd like to hear.

We have used the CPD 030 for several years in a multi-user
laboratory (many students). I have found it extremely reliable. The
only problem we have had was small Teflon pieces detaching from the
magnet valves, thus jamming the gas outlet aperture. This problem
is solved since long. Also the accessories are well made and very
suitable for their purpose (in contrary to the accessories of the
earlier models).

Rolf

========================================================================
Rolf Odselius, PhD, Ass Prof
Electron Microscopy Unit, University Hospital
S-221 85 Lund, Sweden
Phone: +46 46 171075 Fax: +46 46 172975 Mobile phone: +46 10 6705655
Pager (Minicall): +46 740 288992 E-mail: Rolf.Odselius-at-emu.lu.se
URL: http://www.wblab.lu.se/medfak/medinst/emu/
Educational Secretary, SCANDEM URL: http//www.ldc.lu.se/~scandem/




From: Chris Frethem (CBN) :      frethem-at-lenti.med.umn.edu
Date: Thu, 29 Jun 1995 08:09:02 -0500 (CDT)
Subject: Negative sleeves/envelopes

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I agree with recommendation for use of polyethylene negative sleeves
for long term storage and convenience when using light box. Check with
your local poly bag manufacturing companies; I recently ordered 5000
4.125" X 5.5" 1.5 mil sleeves for Polaroid T-55 negatives locally
for $20.37 / 1000. They could probably make other sizes as well but I
didn't ask. Some companies don't want to be bothered with custom sizing
or small orders, so shop around. I don't want to advertise for this
particular supplier, but if you contact me directly I will give you
name and address.

Chris





=======================================================================
Chris Frethem (612)624-4652 (voice)
Cell Biology & Neuroanatomy (612)624-8118 (FAX)
U of MN, Minneapolis e-mail: frethem-at-lenti.med.umn.edu







From: EMLAB-at-opus.mco.edu
Date: Thu, 29 Jun 1995 09:32:55 -0500 (EST)
Subject: Re: Agar bound to slides

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Dear Clint,

Try using glue. Loctite Tissue Adhesive is used all the time for gluing
tissues to aluminium blocks for vibrtome sectioning. It is available thru
Ted Pella.

Good Luck,
Ed Calomeni
Medical College of Ohio
Toledo, OH
emlab-at-opus.mco.edu




From: Walt Bobrowski :      bobroww-at-aa.wl.com
Date: Thu, 29 Jun 1995 10:28:33 -0400 (EDT)
Subject: 'Reduced' Osmium Query

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Mr-Received: by mta PETVAX.MUAS; Relayed; Thu, 29 Jun 1995 10:28:33 -0400
Mr-Received: by mta PETVAX; Relayed; Thu, 29 Jun 1995 10:28:35 -0400
Mr-Received: by mta SRVR01; Relayed; Thu, 29 Jun 1995 10:29:57 -0400
Disclose-Recipients: prohibited

Can someone please differentiate the use of potassium ferrocyanide-reduced OsO4
for immunoelectron microscopy and plain 'ol OsO4 in lower concentration?

Thanks in advance,

Walt Bobrowski
Parke-Davis Research
Ann Arbor, MI






From: rcrang-at-pop.life.uiuc.edu (Richard Crang)
Date: Thu, 29 Jun 1995 10:07:27 -0500
Subject: Containing Unicells for Freeze-Substitution

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We are preparing plant (beet root) protoplasts for transmission electron
microscopy studies and would like to have some of them prepared by means of
freeze substitution. They are in an osmotically adjusted buffer medium as a
cell suspension and, while we can centrifuge them (gently) to condense them
and even freeze them in chilled propane, they will disperse during the
substitution process, and particularly upon warming. Thus, they will be
lost upon the exchange of fluids. We are using a Reichert CS-Auto FS
system. Any suggestions how the material (or any unicellular preparations
for that matter) can be handled to contain them during freeze substitution?
Maintaining the cells on agar is not possible due to the need to keep them
in an osmotic medium prior to freezing. Suggestions greatly appreciated.



**************************
Richard F. E. Crang
Professor of Plant Biology
University of Illinois
(217) 244-3143
**************************





From: Yuan Liang Chen :      cheny-at-mcnc.org
Date: Thu, 29 Jun 1995 12:29:29 -0400
Subject: Re: Gen Info: Starting the next millenia

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Dear Dr. Zaluzec,
My subscription to AAEM.AMC.ANL.GOV was undeliverable yesterday.
I has been off the internet for 6 months and don't know what
happened. Also I can not reach BBS. Please tell me what to do.
Thanks.
YL_CHEN-at-PNL.GOV




From: bafpjec-at-uxa.ecn.bgu.edu (Joyce Craig)
Date: Thu, 29 Jun 1995 11:35:18 -0500
Subject: unsubscribe

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Please take me off the microscopy listserver
Joyce Craig
Biology Department
Chicago State University
9501 King Drive
Chicago, IL 60628





From: emlab-at-ucsco.ucsc.edu (Jon Krupp)
Date: Thu, 29 Jun 1995 10:05:52 -0700
Subject: Nikon Microscope

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Message-Id: {199506291704.KAA01886-at-ucsco.ucsc.edu}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

The following is posted as a courtesy for a colleague not familiar with
this list.

You may recall my request for help identifying a Nikon microscope earlier
this month. As you can see, we were able to find out a lot, due in part to
helpful readers of this list. Thanks to all. This list and all of you are a
great resource for me and many others at UC Santa Cruz.
***************************************************************************


Here is the more complete data on the Nikon X-Y microscope we
communicated about earlier this month:

6x6 Nikon Measuremaster, precision measuring X-Y binocular
microscope system for transmitted and reflected light, X-Y stage allows for
6" movement in each direction, separate Fiber-lite high intensity fiber
optic illumination system (series-180), Nikon digital X-Y counter (CM-65),
manual stage drives, but can be motorized, five turret rotating objective
head with Nikon x2.5 & x10 objectives, Vickers x20 objective, and Zeiss x25
& x100 objectives, tower allows for microscope to be focused up about 6" to
10" above stage (for large samples); microscope was not much used and is in
excellent condition.

Asking Price: $6000

If you're interested in it and have a funding source, let me know.

best wishes,
Othmar Tobisch
408-459-2777
otobisch-at-earthsci.ucsc.edu

*************************************************************************

Jonathan Krupp
Microscopy and Imaging Lab
University of California
Santa Cruz, CA 95064
(408) 459-2477
emlab-at-ucsco.ucsc.edu






From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 29 Jun 1995 16:48:47 -0400
Subject: Re-IntersciAddr

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Message-ID: {n1407677874.79134-at-mse.engin.umich.edu}

Subject: Time: 4:44 PM
OFFICE MEMO Re:IntersciAddr Date: 6/29/95

I believe that Interscience is a division of John Wiley & Sons, 605 Third
Ave., NY, NY, 10158. Tel: 212-850-6000





From: wise-at-vaxa.cis.uwosh.edu
Date: Thu, 29 Jun 1995 16:17:11 +0000
Subject: Re-IntersciAddr

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To all,

Occassionally, I have run across references to saphire or vitreous
carbon knives for ultramicrotomy but I have never seen any for sale. Does
anyone know if such knives were ever (are) commercially available?

Bob Wise


Robert R. Wise, PhD
Director, UWO Electron Microscope Facility
Department of Biology
University of Wisconsin Oshkosh
Oshkosh, WI 54901
(414) 424-3404
(414) 424-1101 fax
wise-at-vaxa.cis.uwosh.edu






From: Clint E. Young :      clintey-at-unixg.ubc.ca
Date: Thu, 29 Jun 1995 23:08:50 -0700 (PDT)
Subject: Quantitative Image Analysis

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To fellow collegues

There does not seem to be a forum (mailing list, discussion group, etc..)
singularly devoted to the subject of quantitative image analysis (Q-IA).
I would, therefore, like to create a mailing list of sorts.

First, let me explain what my lab is doing.

In my lab, quantitative image analysis (Q-IA) is used in combination with
immunohistochemistry and Western Blotting. The purpose of Q-IA, in the
context of our lab work, is to capture images of immunostained tissue and
immunoblots in order to determine the amount of staining and, therefore,
the amount of antigen present.

Q-IA of immunostained tissue can also be used to determine:

A. MACROSCOPIC FIELD
1. Number of cells stained
2. Size of cells
3. Distribution of cells

B. MICROSCOPIC FIELD
1. Amount of antigen present
2. Distribution of antigen
3. Colocalization of different antigens (confocal)

Q-IA of immunoblots, meanwhile, is used primarily for B.1-2.

To perform this type of analysis one needs peripherals (digital camera
and/or flatbed scanner), hardware (PC or Macintosh computer), and
software (NIH Image, Adobe Photoshop, etc...).

Obviously, Q-IA can be performed using many different setups depending on
the combination of peripherals-hardware-software used.

I would like, therefore, to make a list of those interested in this topic
and will circulate this list among the participants.

Please include the following:

1. E-Mail address
2. Computer
3. Image Analysis Software
4. Type of analysis you are CURRENTLY ABLE to perform
5. Question/Comment


----------
The following is an example of what I would send:

clintey-at-unixg.ubc.ca
Macintosh
NIH Image
IHC: A(1,2,3) + B(1,2,3)
WB: B(1,2)
?: I am taking images of Western Blots using reflective light; I've tried
making the WB transparent but the process causes the staining to run or
streak. Does anyone know which method--reflective or transmitted
light--works best for quantifying Western Blots?

----------

Please E-Mail me directly since this message will be sent to various
mailing lists.

I will send participants the compiled list on Monday July 3.

Looking forward to your reply.

Clint Young
Department of Psychiatry
University of British Columbia





From: rms-at-vax.ox.ac.uk
Date: Fri, 30 Jun 1995 11:15:19 +0100
Subject: J. Microsc. Abstracts. Delete message if not interested!

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JTERLET-at-CEMMA.ADELAIDE.EDU.AU, microtoday-at-aol.com, ROSS.MACKENZIE-at-OX.AC.UK,
MICROSCOPY-at-AAEM.AMC.ANL.GOV
Message-ID: {00992A63.3FDF0AA6.23-at-vax.ox.ac.uk}

JOURNAL OF MICROSCOPY ABSTRACTS
DECEMBER 1994 - FEBRUARY 1995

FOR MORE DETAILS ABOUT THE JOURNAL OF MICROSCOPY, CONTACT RMS-at-VAX.OX.AC.UK


Journal of Microscopy, Vol. 176, Pt. 3, December 1994, pp.
181-187.

In situ analysis of microbial consortia in activated sludge
using fluorescently-labelled, rRNA-targeted oligonucleotide
probes and scanning confocal laser microscopy

M. Wagner, B. Assmus, A. Hartmann, P. Hutzler & R. Amann
Lehrstuhl fur Mikrobiologie, Technische Universitat Munchen,
Arcisstrasse 21, D-80290 Munchen, Germany


SUMMARY

Activated sludge flocs are complex consortia of various micro-
organisms. The community structures of samples taken from
municipal sewage treatment plants were characterized using
fluorescently labelled, 16S and 23S rRNA-targeted
oligonucleotide probes in combination with confocal scanning
laser microscopy (CSLM). In comparison with conventional
epifluorescence microscopy, CSLM considerably improved the
capability to visualize directly the spatial distribution of
defined bacterial populations inside the sludge flocs.
Analyses could be performed at high resolution undisturbed by
problems such as autofluorescence or limited spatial
resolution in thick samples. In addition, CSLM was used to
analyse some structural properties of paraformaldehyde-fixed
activated sludge flocs, such as floc size and homogeneity.
Typical floc sizes were found to be in the range between 5 and
50 micrometre. Whereas most of the flocs were completely
colonized by bacteria, there were also examples of flocs
containing gas bubbles or particles in the interior.


Journal of Microscopy, Vol. 176, Pt. 3, December 1994, pp.
188-194.

Scanning interference and confocal microscopy

R. Juskaitis & T. Wilson, Department of Engineering Science,
University of Oxford, Parks Road, Oxford, OX1 3PJ, U.K.


SUMMARY

The form of the interference term image in scanning confocal
and scanning conventional interference microscopes is
identical in all respects including optical sectioning. This
observation is used to obtain confocal images and surface
profiles from conventional scanning interference microscope
images.


Journal of Microscopy, Vol. 176, Pt. 3, December 1994, pp.
195-203.

Time- and wavelength-resolved spectroscopy in two-photon
excited fluorescence microscopy

S. Andersson-Engels, I. Rokahr & J. Carlsson
Department of Physics, Lund Institute of Technology, PO Box
118, S-221 00 Lund, Sweden


SUMMARY

Two-photon excited fluorescence spectroscopy has been
performed at a microscopic scale in combination with normal,
white light microscopy. This gave simultaneously a spectral
resolution of 20nm and a temporal resolution of 20ps, from a
volume element less than 5 micrometre in all three dimensions.
The sample was excited with light from a continuously mode-
locked Ti:sapphire laser that was focused on the sample in a
fluorescence microscope. A polychromator and streak-camera
were used for detection. The method has been used on tissue,
plant and paper samples. It has also been demonstrated how
substances naturally occurring in the samples can be
identified from their spectroscopic properties and the spatial
distribution of these substances can be observed.



Journal of Microscopy, Vol. 176, Pt. 3, December 1994, pp.
204-210.

Intracellular localization of the antitumour drug adriamycin
in living cultured cells: a confocal microscopy study

S. Meschini, A. Molinari, A. Calcabrini, G. Citro & G. Arancia
Department of Ultrastructures, Istituto Superiore di Sanita,
Viale Regina Elena 299, 00161 Rome, Italy


SUMMARY

The intracellular distribution of the anthracyclinic
antibiotic adriamycin in living cultured cells has been
investigated by confocal microscopy.
In human melanoma cells (M14), adriamycin was localized
inside the nuclei. When adriamycin-treated M14 cells were
allowed to recover in a drug-free medium, a complete efflux of
the drug from the nucleus was revealed. In recovered cells, a
weakly fluorescent signal was observed in the perinuclear
region. When M14 cells were recovered in a medium containing
colcemid, a microtubule depolymerizing agent, the drug
transport from the nucleus to the cell periphery appeared to
be inhibited, suggesting that the microtubule network is
strongly involved in drug transport mechanisms. In multidrug-
resistant (MDR) cells the intracellular location of adriamycin
was shown to be noticeably different from that of the parental
wild-type cells. In particular, in resistant human breast
carcinoma cells (MCF-7), adriamycin appeared to be exclusively
located within the cytoplasm, whereas the nuclei were shown to
be completely negative. When adriamycin treatment was
performed in association with MDR revertants, such as
Lonidamine (inhibitor of the energy metabolism) or verapamil
(inhibitor of the P-glycoprotein efflux pump), a marked
enhancement of the cytoplasmic signal was observed in
resistant cells. Under these conditions, adriamycin appeared
concentrated in the perinuclear region, but the nuclei were
still negative. Confocal microscopy proved to be a useful
method for the study of the intracellular transport of
fluorescent substances, such as anthracyclinic antibiotics,
and for the investigation of the multidrug resistance
phenomenon in tumour cells.



Journal of Microscopy, Vol. 176, Pt. 3, December 1994, pp.
211-221.

A versatile tilting device for fluorescence microscopes

J. Bradl, M. Hausmann, B. Schneider, B. Rinke & C. Cremer
Institut fur Angewandte Physik, Albert-Uberle Strasse 3-5,
D-69120 Heidelberg, Germany


SUMMARY

A tilting device for biological specimens (rotation angle up
to 2 pi) especially fluorescence-labelled cell nuclei, was
developed. It consists of a quartz glass capillary and a
mounting adaptor for the microscope stage. The applicability
of the device was tested for several epifluorescence and
confocal scanning laser microscopes. The axis of rotation is
perpendicular to the optical axis of the microscope. The
capillary can be tilted around its axis at any desired angle
or in equiangular steps. This can be done manually or by
remote control using a stepping motor.
The three-dimensional (3-D) image-forming properties of
the capillary system were experimentally examined using an
inverse confocal scanning laser microscope. The results were
compared with measurements obtained from the same microscope
with the standard stage for plane slides with cover glasses.
The measured point spread function suggested that, in spite of
aberration effects, the optical arrangement used allows a gain
in the 3-D resolution by tilting the object.
A low-cost, fully-automated 3-D imaging system was built
on the basis of a conventional epifluorescence microscope with
a cooled black-and-white CCD camera. The system was operated
by a personal computer. The online visualization ('movie') of
rotating objects indicates the feasibility of the system.



Journal of Microscopy, Vol. 176, Pt. 3, December 1994, pp.
222-225.

Continuous wave excitation two-photon fluorescence microscopy

P. E. Hanninen, E. Soini & S. W. Hell
Department of Medical Physics, University of Turku, Center for
Biotechnology, Tykistokatu 6, FIN-20521 Turku, Finland


SUMMARY

Two-photon excitation fluorescence imaging is feasible with
continuous wave lasers. Images of biological specimens are
obtained by employing photon counting in conjunction with an
increasing recording time. The approach allows two-photon
three-dimensional imaging of fluorescently-labelled specimens
with inexpensive lasers.


Journal of Microscopy, Vol. 176, Pt. 3, December 1994, pp.
226-230.

Refractive-index-induced aberrations in two-photon confocal
fluorescence microscopy

H. Jacobsen, P. E. Hanninen, E. Soini & S. W. Hell
Department of Medical Physics, University of Turku, Center for
Biotechnology, Tykistokatu 6, FIN-20521 Turku, Finland


SUMMARY

The effect of refractive index mismatch on the image quality
in two-photon confocal fluorescence microscopy is investigated
by experiment and numerical calculations. The results show a
strong decrease in the image brightness using high-aperture
objectives when the image plane is moved deeper into the
sample. When exciting at 740nm and recording the fluorescence
around 460nm in a glycerol-mounted sample using a lens of a
numerical aperture of 1.4 (oil immersion), a 25% decrease in
the intensity is observed at a depth of 9 micrometre. In an
aqueous sample, the same decrease is observed at a depth of 3
micrometre. By reducing the numerical aperture to 1.0, the
intensity decrease can be avoided at the expense of the
overall resolution and signal intensity. The experiments are
compared with the predictions of a theory that takes into
account the vectorial character of light and the refraction of
the wavefronts according to Fermat's principle. Advice is
given concerning how the effects can be taken into account in
practice.


Journal of Microscopy, Vol. 176, Pt. 3, December 1994, pp.
231-237.

The tetrahedral tip as a probe for scanning near-field optical
microscopy at 30nm resolution

U. C. Fischer, J. Koglin & H. Fuchs
Westfalische Wilhelms Universitat, Physikalisches Institut,
Wilhelm-Klemm-Strasse 10, 48149 Munster, Germany


SUMMARY

The tetrahedral tip is introduced as a new type of probe for
scanning near-field optical microscopy (SNOM). Probe
fabrication, its integration into a scheme of an inverted
photon scanning tunnelling microscope and imaging at 30nm
resolution are shown. A purely optical signal is used for
feedback control of the distance of the scanning tip to the
sample, thus avoiding a convolution of the SNOM image with
other simultaneous imaging modes such as force microscopy. The
advantages of this probe seem to be a very high efficiency and
its potential for SNOM at high lateral resolution below 30nm.



Journal of Microscopy, Vol. 176, Pt. 3, December 1994, pp.
238-244.

Studies of porphyrin containing specimens using an optical
spectrometer connected to a confocal scanning laser microscope

O. Trepte, I. Rokahr, S. Andersson-Engels & K. Carlsson
Physics IV, The Royal Institute of Tech, S-100 44 Stockholm,
Sweden


SUMMARY

A spectrometer has been developed for use with a confocal
scanning laser microscope. With this unit, spectral
information from a single point or a user-defined region
within the microscope specimen can be recorded. A glass prism
is used to disperse the spectral components of the recorded
light over a linear CCD photodiode array with 256 elements. A
regulated cooling unit keeps the detector at 277K, thereby
allowing integration times of up to 60s. The spectral
resolving power ranges from 350 at 400nm to 100 at 700nm.
Since the entrance aperture of the spectrometer has the same
size as the detector pinhole used during normal confocal
scanning, the three-dimensional spatial resolution is
equivalent to that of normal confocal scanning. Light from the
specimen is deflected to the spectrometer by a solenoid
controlled mirror, allowing fast and easy switching between
normal confocal scanning and spectrometer readings.
With this equipment, studies of rodent liver specimens
containing porphyrins have been made. The subcellular
localization is of interest for the mechanisms of photodynamic
therapy (PDT) of malignant tumours. Spectroscopic detection is
necessary to distinguish the porphyrin signal from other
fluorescent components in the specimen. Two different
substances were administered to the tissue, Photofrin, a
haematoporphyrin derivative (HPD) and delta-amino levulinic
acid (ALA), a precursor to photoporphyrin IX and haem in the
haem cycle. Both are substances under clinical trials for PDT
of malignant tumours. Following administration of these
compounds to the tissue, the potent photosensitizer and
fluorescent compound photofrin, or protoporphyrin IX,
respectively, is accumulated. For our study Wistar/Furth rats
were injected either with Photofrin or with ALA 3-5h before
they were killed. The organs were removed directly after and
snap-frozen in carbon dioxide ice with isopentane. No further
staining or fixation procedures were adopted.


Journal of Microscopy, Vol. 176, Pt. 3, December 1994, pp.
245-253.

Modelling of inclined and curved surfaces in the reflection
scanning acoustic microscope

W. Weise, P. Zinin & S. Boseck
Institute for Materials Science and, Structure Research,
Physics Department, University of Bremen, 28334 Bremen,
Germany


SUMMARY

An expression is derived for the output signal when an
inclined plane surface is imaged by the reflection scanning
acoustic microscope, which is modelled as a spherical
transducer. This expression is applied to model non-planar
surfaces. The accuracy of this approach is tested for
perfectly reflecting spherical surfaces. The influence of
inclination on V(z) curves is considered when Rayleigh waves
occur.


Journal of Microscopy, Vol. 176, Pt. 3, December 1994, pp.
254-261.

Scanning force microscopy on live cultured cells: imaging and
force-versus-distance investigations

D. Ricci & M. Grattarola
Dipartimento di Ingegneria Biofisica, ed Elettronica,
Universita degli Studi di Genova, Via Opera Pia 11a, 16145
Genova, Italy


SUMMARY

Extensive measurements with the scanning force microscope
(SFM) on living cells in their native liquid environment are
described with the purpose of critically assessing the extent
of the interaction between the SFM tip and the (soft) cell
materials and the effect of such interaction on topographic
information. Images are obtained under various force
conditions and systematically correlated with force-versus-
distance curves. As a result, detailed indications about tip
indentation are given, thickness estimates deduced and
identification of submembranous cytoplasmic structures
suggested.


Journal of Microscopy, Vol. 176, Pt. 3, December 1994, pp.
262-275.

In vivo analysis of angiogenesis and revascularization of
transplanted pancreatic islets using confocal microscopy

F. A. Merchant, S. J. Aggarwal, K. R. Diller & A. C. Bovik
Biomedical Engineering Research Program, ENS 612, University
of Texas, Austin 78712-1084, U.S.A.


SUMMARY

A technique to measure angiogenesis and revascularization in
pancreatic islets transplanted at the renal subcapsular site
in the rat has been developed. In vivo imaging of the
microcirculation of transplanted pancreatic islets was
conducted using a confocal scanning laser microscope (CSLM) to
achieve optical sectioning through the graft in order to
perform a computer reconstruction of the three-dimensional
neovascular morphology. Individual islets were harvested by
enzymatic digestion of excised pancreas from Fischer 344 rats.
Isolated islets were cultured for 24h, and approximately 300-
350 islets were transplanted at the renal subcapsular site of
the left kidney in an anaesthetized rat. Six to 14 days post-
transplantation, the animal was anaesthetized and prepared for
in vivo imaging of the microvasculature on a Zeiss LSM-10.
Optical contrast of the microvasculature was enhanced by the
administration of fluorescein-labelled dextran into the
circulating blood. The transplant site was identified and
serial sections were obtained through the vascular bed at
varying z-intervals. Complementary fluorescence video images
were also obtained via a silicon intensifier tube camera
mounted on the CSLM. At completion of the imaging procedure,
the kidney was returned into the body cavity, the area was
sutured and the animal was allowed to recuperate for
subsequent examinations. Image processing algorithms, such as
grey-level thresholding, median filtering, skeletonization and
template matching, were applied to compute the vessel density
and diameters and extrapolated to measure 3-D vessel lengths
and the tortuosity index of the neovasculature.



Journal of Microscopy, Vol. 176, Pt. 3, December 1994, pp.
276-280.

Optoelectronic detector probes for scanning near-field optical
microscopy

H. U. Danzebrink
Physikalisch-Technische Bundesanstalt, Bundesallee 100,
D-38116 Braunschweig, Germany


SUMMARY

A brief explanation of the optoelectronic probe concept and a
comparison between the implementation of passive waveguide
probes and optoelectronic probes in scanning near-field
optical microscopy (SNOM) is presented. The first probe
realizations using cleaved semiconductor crystals and the work
at present in progress using microfabricated Si pyramids are
described. These crystals with evaporated metal electrodes
forming a slit aperture with subwavelength dimensions work as
metal-semiconductor-metal photodetectors. Their optical
detection behaviour is investigated by measuring the intensity
distribution of a laser focal point. Measurements where the
external bias voltage is changed show that it is possible to
modify the detection behaviour of the device because of the
varying depletion widths. The last part of the article
describes a concept where pyramidal probes should function
simultaneously as senors for scanning force microscopy (SFM)
to measure topography and as optoelectronic probes for
scanning near-field optoelectronic microscopy (SNOEM).


Journal of Microscopy, Vol. 176, Pt. 3, December 1994, pp.
281-286.

Imaging in the far-red with electronic light microscopy:
requirements and limitations

C. Cullander
School of Pharmacy S926, University of California, San
Francisco, CA 94143-0446, U.S.A.


SUMMARY

The acquisition of simultaneous dual confocal images with red
and far-red light has both advantages (e.g. lower
autofluorescence) and limitations. An understanding of these
requisites is necessary to acquire high-quality images and to
avoid the misinterpretation of experimental data. The poor
detection of far-red light mandates a high optical transfer
efficiency for the system, thus the transmittance of the
objective lens and its axial and lateral chromatic aberration
in the far-red are important factors for consideration. This
technical note is an attempt to 'demystify' the process of
filter set design for confocal microscopy by discussing the
considerations that went into the construction of a filter set
for use with the reagents cyanine 3.18 (Cy3) and cyanine 5.18
(Cy5), and thus to encourage users to look beyond the
multipurpose designs available commercially. The 568-nm laser
line exciting Cy3 is at its emission maximum, which limits the
collectable Cy3 fluorescence. High-transmission optical
filters with sharp bandpass cutoffs are thus desirable for
maximum light throughput. Light path mirror efficiency rapidly
degrades above 700nm, but the loss of this portion of the Cy5
emission spectrum is acceptable since the fluorophore is very
bright, and these very long wavelengths are also likely to
introduce aberration. While resolution is decreased with far-
red light, there is also greater penetration and less
scattering, and it is thus possible to obtain high-quality
images from deeper within the specimen. Although only one make
and model of confocal microscope (the Bio-Rad MRC-600) is
considered, similar considerations pertain to the design of
filter sets for any confocal microscope that accommodates
user-installed filters.



Journal of Microscopy, Vol. 176, Pt. 3, December 1994, pp.
287-299.

Simultaneous confocal recording of multiple fluorescent labels
with improved channel separation

K. Carlsson, N. Aslund, K. Mossberg & J. Philip
Physics IV, The Royal Institute of Tech, S-100 44 Stockholm,
Sweden


SUMMARY

Confocal microscopes are often used to study specimens
labelled with fluorophores. A commonly used method for
simultaneous recording of the distribution of multiple
fluorophores is to divide the fluorescent light emitted by the
specimen into different wavelength regions using dichroic and
bandpass filters. These different wavelength regions are then
distributed to multiple detectors. However, fluorophores often
result in considerable cross-talk between channels. A new
technique, intensity-modulated multiple-beam scanning (IMS)
microfluorimetry, can be used to reduce this cross-talk
substantially.
The IMS technique is implemented with two laser beams of
different wavelengths, intensity-modulated at different
frequencies, which illuminate the specimen simultaneously. The
two laser wavelengths predominantly excite one fluorophore
each. Fluorescent light from the specimen is divided into two
wavelength regions (red and green) which are detected by two
photomultiplier tubes. The output signals from the
photomultiplier tubes are connected to lock-in amplifiers. The
effect of using modulated laser beams, in combination with
lock-in amplifiers, is strongly to reduce the cross-talk
between channels. The performance of the IMS technique using
various types of specimen is compared with the results
obtained using the conventional multi-detector design.




Journal of Microscopy, Vol. 177, Pt. 1, January 1995, pp. 1-6.

In vivo determination of fibril orientation in plant cell
walls with polarization CSLM

J.-P. Verbelen & D. Stickens
Department of Biology, University of Antwerp (UIA),
Universiteitsplein 1, B-2610 Wilrijk-Antwerpen, Belgium


SUMMARY

Congo Red fluorescence is used to detect cellulose in the wall
of plant cells. The orientation of the cellulose fibrils is
determined by using polarized light for excitation. The
absorption characteristics of Congo Red make this approach a
method of choice for applications with any standard confocal
scanning laser microscopy (CSLM). The semi-quantitative
character of CSLM observations combined with the non-toxicity
of the stain allow a very fast and reliable assessment of
cellulose orientation in the wall of living plant cells.



Journal of Microscopy, Vol. 177, Pt. 1, January 1995, pp.
7-17.

Three-dimensional reconstruction of the human renal glomerulus

K. Preston Jr, B. Joe, R. Siderits & J. Welling
Kensal Corporation, 5055 East Broadway (Suite C206), Tucson AZ
85711, U.S.A.


SUMMARY

The capillary bed of human renal glomerulus is one of the more
complex capillary structures in the human body. This paper
illustrates three-dimensional reconstruction of the capillary
bed from serial sections. It shows that, although traditional
methods of three-dimensional rendering by computer fail to
handle the complexities of the capillary structure, new
methods based on filtering using three-dimensional
mathematical morphology are capable of revealing previously
unseen details. This is done at the expense of eliminating
fine structure (small capillaries). An error analysis allows
the degree to which fine details are lost to be estimated.



Journal of Microscopy, Vol. 177, Pt. 1, January 1995, pp.
18-30.

Quantitative water mapping of cryosectioned cells by electron
energy loss spectroscopy

S. Q. Sun, S.-L. Shi, J. A. Hunt & R. D. Leapman
Health & Human Services, Public Health Service, National
Institutes of Health, Building 13 Room 3W13, Bethseda MD
20892, U.S.A.


SUMMARY

A direct technique based on electron energy-loss spectroscopy
(EELS) in the scanning transmission electron microscope (STEM)
has been developed to map subcellular distributions of water
in frozen-hydrated biological cryosections. Previously,
methods for water determination have been indirect, in that
they have required the cryosections to be dehydrated first.
The new approach makes use of spectrum-imaging, where EELS
data are collected in parallel at each pixel. Several
operations are required to process the spectra including:
subtraction of the detector dark current, deconvolution by the
detector point-spread function, removal of plural inelastic
scattering and correction for the support film. The resulting
single scattering distributions are fitted to standard
reference spectra at each pixel, and water content can be
determined from the fitting coefficients. Although the
darkfield or brightfield image from a hydrated cryosection
shows minimal structure, the processed EELS image reveals
strong contrast due to variation in water content. Reference
spectra have been recorded from the major biomolecules
(Protein, lipid, carbohydrate, nucleic acid) as well as from
vitrified water and crystalline ice. It has been found that
quantitative results can be obtained for the majority of
subcellular compartments by fitting only water and protein
reference spectra, and the accuracy of the method for these
compartments has been estimated as plus/minus 3.5%. With the
present instrumentation the maximum allowed dose of 2000e/nm2
limits the useful spatial resolution to around 80nm plus/minus
5% precision at a single pixel. By averaging pixel intensities
a value of 56.8% with a precision of plus/minus 2.0% has been
determined for the water content of liver mitochondria. The
water mapping technique may prove useful for applications to
cell physiology and pathophysiology.



Journal of Microscopy, Vol. 177, Pt. 1, January 1995, pp.
31-42.

Hybrid scanning transmission electron/scanning tunnelling
microscope system for the preparation and investigation of
biomolecules

H. F. Knapp, R. Wyss, R. Haring, C. Henn, R. Guckenberger & A.
Engel
Maurice E Muller Institut fur, Hochauflosende
Elektronenmikroskopie, Universitat Basel, Klingelbergstrasse
70, CH-4056 Basel, Switzerland


SUMMARY

A hybrid scanning transmission electron/scanning tunnelling
microscope vacuum system is introduced, which allows freeze
drying and metal coating of biological samples and their
simultaneous observation by scanning transmission electron
microscopy and scanning tunnelling microscopy (STM). Different
metal coatings and STM tips were analysed to obtain the
highest possible resolution for such a system. Bovine liver
catalase was used as a test sample and the STM results are
compared to a molecular scale model.


Journal of Microscopy, Vol. 177, Pt. 1, January 1995, pp.
43-52.

Backscattered electron imaging of the undersurface of
resin-embedded cells by field emission scanning electron
microscopy

R. G. Richards & I. Ap Gwynn
AO/ASIF Research Institute, Clavadelerstrasse, CH-7270 Davos
Platz, Switzerland


SUMMARY

In this study backscattered electron (BSE) imaging was used to
display cellular structures stained with heavy metals within
an unstained resin by atomic number contrast in successively
deeper layers. Balb/c3T3 fibroblasts were cultured on either
13mm discs of plastic Thermanox, commercially pure titanium or
steel. The cells were fixed, stained and embedded in resin and
the disc removed. The resin block containing the cells was
sputter coated and examined in a field-emission scanning
electron microscope. The technique allowed for the direct
visualization of the cell undersurface and immediately
overlying areas of cytoplasm through the surrounding embedding
resin, with good resolution and contrast to a significant
depth of about 2 micrometre, without the requirement for
cutting sections. The fixation protocol was optimized in order
to increase heavy metal staining for maximal backscattered
electron production. The operation of the microscope was
optimized to maximize the number of backscattered electrons
produced and to minimize the spot size. BSE images were
collected over a wide range of accelerating voltages (keV),
from low values to high values, to give 'sections' of
information from increasing depths within the sample. At 3-
4keV only structures a very short distance into the material
were observed, essentially areas of cell attachment to the
removed substrate. At higher accelerating voltages information
on cell morphology, including in particular stress fibres and
cell nuclei, where heavy metal were intensely bound, became
more evident. The technique allowed stepwise 'sectional'
information to be acquired. The technique should be useful for
studies on cell morphology, cycle and adhesion with greater
resolution than can be obtained with any light-microscope-
based system.



Journal of Microscopy, Vol. 177, Pt. 1, January 1995, pp.
53-67.

Tomographic reconstruction of the cross-sectional refractive
index distribution in semi-transparent birefringent fibres

T. C. Wedberg & W. C. Wedberg
Physics Department, University of Bergen, Allegt 55, N-5007
Bergen, Norway


SUMMARY

Optical diffraction tomography (ODT) is used to reconstruct
the complex refractive index distribution in cross-sections of
semi-transparent, birefringent fibres. The selected fibres
were polymer and animal fibres of either circular or non-
circular cross-section with average thicknesses in the range
8-110 micrometre. This choice of samples was made to
illustrate the imaging capabilities of ODT, and also to
demonstrate some potential applications of the technique. The
images representing the reconstructed refractive index
distributions have a spatial resolution of about 2 micrometre,
and show noticeable image contrast for refractive index
variations of about 0.001. The ODT reconstructions compare
well with refractive index information provided with the
samples, and with scanning electron micrographs of cross-
sections of the same fibre samples. From these results it
appears that ODT can be used to reconstruct the complex
refractive index distribution in cross-sections of semi-
transparent birefringent fibres.



Journal of Microscopy, Vol. 177, Pt. 1, January 1995, pp.
68-76.

Computer simulated high-resolution transmission electron
microscopy (HRTEM) in tourmaline

E. A. Ferrow
Avdelningen for Mineralogi och Petrologi, Geologiska
Institutionen, Lund Universitet, Solvegatan 13, 223 62 Lund,
Sweden


SUMMARY

The contrast distributions observed in high-resolution
transmission electron microscopy (HRTEM) images of tourmaline
depend on the types and magnitudes of the exchange components
present and on the degree of atom overlap along the direction
of observation. Furthermore, the fractional atomic coordinates
in tourmalines are valid only for the specific specimen
refined. These properties make the interpretation of
experimental HRTEM images of tourmaline using image simulation
if not impossible at least extremely difficult. A correct
interpretation of experimental HRTEM images of tourmaline is
possible provided the structural refinement data on the same
crystal are available. Nevertheless, it is possible to
interpret the experimental HRTEM images of tourmaline if the
composition of the structural model chosen during image
simulation approximates the composition of the specimen
studied by electron microscopy. A good control of the
composition of the specimen studied and an appropriate choice
of a structural model for image simulation are therefore as
important as properly controlling specimen thickness, specimen
tilt, beam tilt and objective lens defocus.



Journal of Microscopy, Vol. 177, Pt. 1, January 1995, pp.
77-84.

Confocal microscopy in the analysis of the etched nuclear
particle tracks in polymers

J. Jakes, P. Gais & H. Schraube
Institut fur Strahlenschutz, GSF-Neuherberg, Postfach 1129,
D-85758 Oberschleissheim, Germany


SUMMARY

The possibility of the morphometric analysis of etched tracks,
induced by protons and alpha particles in the organic polymer
allyl diglycol carbonate (CR-39), using the confocal scanning
laser microscope (CSLM), was studied. The detectors were
investigated in two groups of irradiation experiments, namely:
(a) irradiated with mono-energetic neutrons of energy 1.2MeV,
(b) exposed to the alpha radiation from 222Rn and its progeny.
Both groups were irradiated at normal incidence. Radiation-
induced latent tracks were electrochemically etched, and their
morphometric parameters were investigated in the reflection
mode by using the 488nm spectral line of an argon ion laser. A
constant number of up to 200 optical sections in Z-scan mode
was taken through each selected etched track at vertical
spacings of 0.642 micrometre. Successive reconstructions of Z-
sections were used to determine the following parameters: the
mean radius of the opening channel, the maximum diameter and
the length of the track, and the angle of the track wall to
the surface of the sample. The results show that tracks
produced by alpha particles differ from those induced by
protons. The radius of the opening channel of alpha-particle-
induced tracks ranges from 7.9 to 11 micrometre, whereas for
protons the same parameter ranges between 2.0 and 3.8
micrometre for a specific electrochemical etch procedure.



Journal of Microscopy, Vol. 177, Pt. 1, January 1995, pp.
85-89.

A simple method for overcoming some problems when observing
thick reflected biological samples with the confocal scanning
laser microscope

C. Rumio, M. Morini, J. R. Miani, I. Barajon & P. Castano
Institute of Human Anatomy, Via Mangiagalli 31, 20133 Milano,
Italy


SUMMARY

A simple device is described, which allows the range of depth
of scanning to be reduced when observing thick reflecting
biological specimens with a confocal scanning laser microscope
(CSLM). Thick histological sections of human skin and rat
brain stem were mounted between two coverslip ('sandwich
style') and the optical tomography was performed from both
sides by turning the 'sandwich' upside-down. The samples were
impregnated using standard Golgi-Cox, 'rapid Golgi' or other
silver methods. The ability to turn the sandwich upside-down
is particularly useful when the reflective structure inspected
is deep inside the section, i.e. near the lower surface of the
specimen, or when it is opaque to the laser beam of
excessively reflective.


Journal of Microscopy, Vol. 177, Pt. 1, January 1995, pp.
90-92.

Thick section preparation using a silicon-rubber-based sealant

H. Cox, C. Walker & C. V. Howard
Department of Orthopaedic & Accident Surgery, Royal Liverpool
University Hospital, Prescot Street, PO Box 147, Liverpool,
L69 3BX


SUMMARY

A method has been developed, using a silicon-rubber-based
sealant, which allows 2-3-mm-thick specimens to be maintained
in a protected fluid environment for a number of months,
without risk of dehydration. Following this, the specimen can
be retrieved, stained, embedded and sectioned further. For
example, 2-mm-thick sections of fixed unstained bone are
easily examined by means of epi-illuminated polarized light
and fluorescence microscopies using either conventional or
confocal optics. The method could easily be extended to other
tissues, for example brain tissue.



Journal of Microscopy, Vol. 177, Pt. 2, February 1995, pp. .

A method to compensate for light attenuation with depth in
three-dimensional DNA image cytometry using a confocal
scanning laser microscope

A. Liljeborg, M. Czader & A. Porwit
Physics IV, The Royal Institute of Tech, S-100 44 Stockholm,
Sweden


SUMMARY

A method to compensate for attenuation of detected light with
increased depth of the collected optical section, and its
application in three-dimensional (3-D) DNA image cytometry is
described. The method is based on studying the stack of 2-D
histograms that ca be formed from each consecutive pair of
sections in a stack of optical serial sections. An attenuation
factor is calculated interactively and a new compensated
section series is computed. Formalin-fixed paraffin-embedded
rat tissue was stained with propidium iodide. Each cell
nucleus is extracted by thresholding and its total intensity
is calculated. The coefficient of variation (CV) of the total
intensity of all cells in each stack is computed. For
comparison the CV of the same cells is computed in the
uncompensated stacks. This study shows a significantly lower
CV for the compensated data, thus contributing to the accuracy
of DNA quantification in 3-D DNA image cytometry.


Journal of Microscopy, Vol. 177, Pt. 2, February 1995, pp. .

Microfractography of granitic rocks under confocal scanning
laser microscopy

M. Montoto, A. Martinez-Nistal, A. Rodriguez-Rey, N.
Fernandez-Merayo & P. Soriano
University of Oviedo, Department of Geology, Group of
Petrophysics, 33005 Oviedo, Spain


SUMMARY

Scanning laser microscopy, in the confocal mode (CSLM) has
been applied to a granitic rock to characterize its fissure
space. The technique provides a unique three-dimensional
picture of the rock microfractomography. CSLM is unique in
observing fine details of the fractographic network
(connectivity, tortuosity, etc.), its geometry and its
relation to other rock-forming components.
The fractographic images with standard fluorescence
microscopy are compared with those obtained with CSLM. The
examples presented emphasize the advantages of CSLM: three-
dimensional visualization of the microfractographic network,
crack connectivity, automatic evaluation of direction and
slope of fissures.
These studies are related to the migration of
radionuclides in the geosphere. The relations between
potentially water-conducting open fissures and the rock-
forming minerals provide a means of modelling the
'radionuclide retardation mechanism', a security factor in
their definitive storage in rock masses.


Journal of Microscopy, Vol. 177, Pt. 2, February 1995, pp. .

PHOEBE, a prototype scanning laser-feedback microscope for
imaging biological cells in aqueous media

T. L. Wong, S. L. Sabato & A. Bearden
Division of Neurobiology, Department of Molecular & Cell
Biology, 229 Stanley Hall, University of California at
Berkeley, Berkeley CA 94720-3206, U.S.A.


SUMMARY

Based on the principle of laser-feedback interferometry (LFI),
a laser-feedback microscope (LFM) has been constructed,
capable of providing an axial (z) resolution of a target
surface topography of approximately 1nm and a lateral (x,y)
resolution of approximately 200nm when used with a high-
numerical-aperture oil-immersion microscope objective. LFI is
a form of interferometry in which a laser's intensity is
modulated by light re-entering the illuminating laser.
Interfering with the light circulating in the laser resonant
cavity, this back-reflected light gives information about an
object's position and reflectivity. Using a 1-mW He-Ne
(wavelength=632.8nm) laser, this microscope (PHOEBE) is
capable is obtaining 256x256-pixel images over fields from
10x10 micrometre to 120x120 micrometre in approximately 30s.
An electrochemical feedback circuit holds the optical
pathlength between the laser output mirror and a point on the
scanned object constant; this allows two types of images
(surface topography and surface reflectivity) to be obtained
simultaneously. For biological cells, imaging can be
accomplished using back-reflected light originating from small
refractive-index changes (} 0.02) at cell membrane/water
interfaces; alternatively, the optical pathlength through the
cell interior can be measured point-by-point by growing or
placing a cell suspension on a higher-reflecting substrate
(glass or silicon wafer). Advantages of the laser-feedback
microscope in comparison to other confocal optical microscopes
include: simplicity of the single-axis interferometric design;
the confocal property of the laser-feedback microscope (a
virtual pinhole), which is achieved by the requirement that
only light that re-enters the laser meeting the stringent
frequency, spatial (TEM00), and coherence requirements of the
laser cavity resonator mode modulate the laser frequency; and
the improved axial resolution, which is based on
interferometric measurement of optical amplitude and phase
rather than by use of a pinhole as in other types of confocal
microscopes.



Journal of Microscopy, Vol. 177, Pt. 2, February 1995, pp. .

Imaging periodic surface relief structures

J. T. Sheridan & T. O. Korner
TP 680, Institute for System Engineering and, Informatics
Science R&D, Joint Research Centre JRS/CCR, I-21020 Ispra
(VA), Italy


SUMMARY

Because of shadowing, multiple scatter and polarization
effects, the interpretation of images of grating with fine
periods, isolated deep structures, and multiple scattering
volume objects is seriously complicated. In this paper a
review of methods used to model such effects is presented.
Periodic surface relief gratings are of particular current
importance because of the possibility of producing calibration
samples using them. Several examples which illustrate
electromagnetic volume effects are examined. General trends
which help in validating the use of Fourier-transform-based
scalar transmittance theory are then indicated. The angular
spectrum approach, which can be used , together with a scatter
function generated using the rigorous electromagnetic theory,
to calculate coherent, partially coherent and confocal images
of volume objects, is also discussed.


Journal of Microscopy, Vol. 177, Pt. 2, February 1995, pp. .

Application of confocal laser microscopy and three-dimensional
Voronoi diagrams for volume and surface area estimates of
interphase chromosomes

R. Eils, E. Bertin, K. Saracoglu, B. Rinke, E. Schrock, Y.
Usson, M. Robert-Nicoud, E. H. K. Stelzer, J.-M. Chassery, T.
Cremer & C. Cremer
Institut fur Angewandte Physik, Albert-Uberle Strasse 3-5,
D-69120 Heidelberg, Germany


SUMMARY

This study demonstrates the use of Voronoi tessellation
procedures to obtain quantitative morphological data for
chromosome territories in the cell nucleus. As a model system,
chromosomes 7 and X were visualized in human female amniotic
fluid cell nuclei by chromosomal in situ suppression
hybridization with chromosome-specific composite probes. Light
optical serial sections of 18 nuclei were obtained with a
confocal scanning laser fluorescence microscope. A three-
dimensional (3-D) tessellation of the image volumes defined by
the stack of serial sections was then performed. For this
purpose a Voronoi diagram, which consists of convex polyhedra
structured in a graph environment, was built for each nucleus.
The chromosome territories were then described by three
morphological parameters, i.e. volume, surface area and a
roundness factor (shape factor). The complete evaluation of a
nucleus, including the calculation of the Voronoi diagram, 3-D
visualization of extracted territories using computer graphic
methods and parameterization was carried out on a Silicon
Graphics workstation and was generally completed within 5 min.
The geometric information obtained by this procedure revealed
that both X- and 7-chromosome territories were similar in
volume. Roundness factors indicated a pronounced variability
in interphase shape for both pairs of chromosomes. Surface
estimates showed a significant difference between the two X-
territories but not between chromosome 7-territories.


Journal of Microscopy, Vol. 177, Pt. 2, February 1995, pp. .

Forbidden light scanning near-field optical microscopy

H. Heinzelmann, B. Hecht, L. Novotny & D. W. Pohl
Institut fur Physik, Universitat Basel, Klingelbergstrasse 82,
4056 Basel, Switzerland


SUMMARY

Near-field optics (NFO) opens the door to light microscopy
techniques with resolutions well beyond the diffraction limit.
The richness of optical investigations is now applicable on a
near-molecular level. Among the novel scanning near-field
optical microscopy (SNOM) schemes, the most prominent
representatives are aperture SNOM and scanning tunnelling
optical microscopy (STOM or PSTM).
New experimental and theoretical work has to be performed
to study the phenomena specific to NFO. One such example is
the angular dependence of light emission in aperture SNOM. The
detection of radiation at angles greater than the critical
angle of total internal reflection alpha=arcsin(1/n), where n
is the sample refractive index, can represent a microscopy
scheme that combines the respective advantages of both
aperture SNOM and STOM. Recent experiments have demonstrated
the expected exponential dependence of light intensity on gap
width (for fixed emission angle). The decay length as a
function of alpha is in agreement with the Fresnel description
of the evanescent field when total reflection occurs at an
interface. These investigations were additionally motivated by
calculations based on the multiple multipole method.



Journal of Microscopy, Vol. 177, Pt. 2, February 1995, pp. .

Automated correction of linear deformation due to sectioning
in serial micrographs

T. Jansson, T. Gustavsson, M. Rydmark, C.-H. Berthold, R.
Pascher & T. Skoglund
Department of Applied Electronics, Chalmers University of
Technology, S-412 96 Goteborg, Sweden


SUMMARY

This paper describes an objective and automatic method for
detection and correction of sectioning deformations in
digitized micrographs, as well as an evaluation of the method
applied to light and electron microscopic images of semi-thin
and ultra-thin serial sections from brain cortex. The
detection is based on matching of image subregions and the
deformation model is bi-linear, i.e. two first-order
polynomials are used for modelling compression/expansion in
perpendicular directions. The procedure is applicable to
prealigned serial two-dimensional sections and is primarily
aimed at three-dimensional reconstruction of tissue samples
consisting of a large number of cells with random distribution
and morphology.


Journal of Microscopy, Vol. 177, Pt. 2, February 1995, pp. .

Comparison of three-dimensional imaging properties between
two-photon and single-photon confocal fluorescence microscopy

Min Gu & C. J. R. Sheppard
Physical Optics Department, School of Physics, The University
of Sydney, NSW 2006, Australia


SUMMARY

The imaging performance in single photon (1-p) and two-photon
(2-p) fluorescence microscopy is described. Both confocal and
conventional systems are compared in terms of the three-
dimensional (3-D) point spread function and the 3-D optical
transfer function. Images of fluorescent sharp edges and
layers are modelled, giving resolution in transverse and axial
directions. A comparison of the imaging properties is also
given for a 4Pi confocal system. Confocal 2-p 4Pi fluorescence
microscopy gives the best axial resolution in the sense that
its 3-D optical transfer function has the strongest response
along the axial direction.


Journal of Microscopy, Vol. 177, Pt. 2, February 1995, pp. .

Double pulse fluorescence lifetime imaging in confocal
microscopy

M. Muller, R. I. Ghauharali, K. Visscher, T. D. Visser & G. J.
Brakenhoff
Department of Molecular Cytology, University of Amsterdam,
Plantage Muidergracht 14, 1018 TV Amsterdam, The Netherlands


SUMMARY

A theoretical analysis of a new technique for fluorescence
lifetime measurement, relying on (near steady state)
excitation with short optical pulses, is presented.
Application of the technique to confocal microscopy enables
local determination of the fluorescence lifetime, which is a
parameter sensitive to the local environment of fluorescent
probe molecules in biological samples. The novel technique
provides good time resolution, since it relies on the laser
pulse duration, rather than on electronic gating techniques,
and permits, in combination with bilateral confocal microscopy
and the use of a (cooled) CCD, sensitive signal detection over
a large dynamic range. The principle of the technique is
discussed within a theoretical framework. The sensitivity of
the technique is analysed, taking into account:
photodegradation, the effect of the laser repetition rate and
the effect of non-steady-state excitation. The features of the
technique are compared to more conventional methods for
fluorescence lifetime imaging.


Journal of Microscopy, Vol. 177, Pt. 2, February 1995, pp. .

Near field imaging: some attempts to define an apparatus
function

D. Courjon
Laboratoire d'Optique PM Duffieux, Associe au CNRS URA-214,
UFR des Sciences et des Techniques, 25030 Besancon Cedex,
France


SUMMARY

Near-field microscopy is a promising new tool capable of
imaging details smaller than the wavelength. The mechanism of
imaging is analysed and an overview of the apparatus functions
which could be used to define an image quality criterion is
given.





From: rms-at-vax.ox.ac.uk
Date: Fri, 30 Jun 1995 11:14:29 +0100
Subject: Abstracts for J Microsc. Delete this message if not interested!

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From: DRStadden:R_D:Armstrong
Date: 6-30-95 7:20am
Subject: FEG SEM for EDX?

Contents Retrieved from Microscopy Listserver Archives
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To: MICROSCO:CENTRAL
cc: DRStadden:R_D:Armstrong
Subj: FEG SEM for EDX?
------------------------------------------------------------------
A friend in academia is preparing to purchase an SEM to serve broad
interests in the sciences. He is wondering whether the field emission
gun instruments work as well for EDX of elements on the light side, such
as sodium and magnesium. Not too concerned with B,C,N,O and F. For
resolution and low KV work, he'd obviously like to have the FEG. Any
thoughts?

Dave Stadden

DRStadde+aCENTRAL%ARMSTRONG-at-MCIMAIL.COM
PH: 717-396-5109
FAX: 717-396-5865





From: rms-at-vax.ox.ac.uk
Date: Fri, 30 Jun 1995 13:19:47 +0100
Subject: More J. Microsc. Delete message now if unwanted!

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Journal of Microscopy
ABSTRACT FOR MAY 1995

Journal of Microscopy, Vol. 178, Pt. 2, May 1995, pp. 93-100.

Limits of electron probe formation

A. V. Crewe
The Enrico Fermi Institute and The Department of Physics, The University of Chicago,
5640 S. Ellis Avenue, Chicago, IL 60637, U.S.A.

Summary
The performance of many electron optical instruments is fundamentally limited by the
dimensions of the focused probe. This is true of the scanning electron microscope
and the scanning transmission electron microscope and, by inference, it may affect the
transmission electron microscope. There has been very little improvement over the
past few years and it seems reasonable to look for the explanation. It is possible to
arrive at some simple expressions for the limiting performances of conventional
instruments in a way that is independent of the design details and depends upon
practical limits of field strength. Experiment and theory also appear to be in
agreement with the fact that the limit for high-voltage instruments has been reached,
although there is still room for improvement for low voltages.


Journal of Microscopy, Vol. 178, Pt. 2, May 1995, pp. 101-106

Environmental scanning electron microscopy of marine aggregates

D. M. Lavoie,* B. J. Little,* R. I. Ray,* R. H. Bennett, M. W. Lambert, V. Asper &
R. J. Baerwald
*Code 7333, Code 7430, Naval Research Laboratory, Stennis Space Center, MS
39529, U.S.A., Center for Marine Science, University of Southern Mississippi, Stennis
Space Center, MS 39529, U.S.A., Department of Biology, University of New Orleans,
New Orleans, LA 70148, U.S.A.

Summary
Marine aggregates were examined for the first time in the hydrated state using an
environmental scanning electron microscope (ESEM). Sample preparation consisted
of fixation followed by rinsing with distilled water to remove excess salts and fixative.
Aggregates were continuously observed at resolutions comparable to conventional
scanning electron microscopy through stages of hydration, from completely immersed
to desiccated. Because no metallic coating is required, energy-dispersive X-ray
spectroscopy (EDXS) can be used to analyse rapidly constituent elements occurring
at low concentrations with no spectral interference. Subtle differences in mineral
particles were seen in both EDXS spectra and in direct observation of relative
hydration, reflecting apparent differences in mineralogy. ESEM enabled examination
of effects of desiccation and rehydration on individual particles composed primarily of
hydrated polymer and eliminated dehydration artefacts in delicate organisms.


Journal of Microscopy, Vol. 178, Pt. 2, May 1995, pp. 107-119

Mechanism of image formation for thick biological specimens: exit wavefront
reconstruction and electron energy-loss spectroscopic imaging

K. F. Han, J. W. Sedat & D. A. Agard
Graduate Group in Biophysics, Department of Biochemistry and Biophysics, and the
Howard Hughes Medical Institute, University of California at San Francisco, San
Francisco, CA 94143-0448, U.S.A.

Summary
With increasing frequency, cellular organelles and nuclear structures are being
investigated at high resolution using electron microscopic tomography of thick sections
(0.3-1.0 m). In order to reconstruct the structures in three dimensions accurately
from the observed image intensities, it is essential to understand the relationship
between the image intensity and the specimen mass density. The imaging of thick
specimens is complicated by the large fraction of multiple scattering which gives rise
to incoherent and partially coherent image components. Here we investigate the
mechanism of image formation for thick biological specimens at 200 and 300 keV in
order to resolve the coherent scattering component from the incoherent (multiple
scattering) components.
Two techniques were used: electron energy-loss spectroscopic imaging (ESI)
and exit wavefront reconstruction using a through-focus series. Although it is
commonly assumed that image formation of thick specimens is dominated by
amplitude (absorption) contrast, we have found that for conventionally stained
biological specimens phase contrast contributes significantly, and that at resolutions
better than 10 nm, superposed phase contrast dominates. It is shown that the
decrease in coherent scattering with specimen thickness is directly related to the
increase in multiple scattering. It is further shown that exit wavefront reconstruction
can exclude the microscope aberrations as well as the multiple scattering component
from the image formation. Since most of the inelastic scattering with these thick
specimens is actually multiple inelastic scattering, it is demonstrated that exit
wavefront reconstruction can act as a partial energy filter. By virtue of excluding the
multiple scattering, the 'restored' images display enhanced contrast and resolution.
These findings have direct implications for the three-dimensional reconstruction
of thick biological specimens where a simple direct relationship between image
intensity and mass density was assumed, and the aberrations were left uncorrected.


Journal of Microscopy, Vol. 178, Pt. 2, May 1995, pp. 120-124

Electrophoretic transfer of protein-pigment complexes from non-denaturing
gels to electron microscopy grids

S. Gr„ber & M. K. Lyon
Molecular, Cellular and Developmental Biology, Campus Box 347, University of
Colorado, Boulder, CO 80309, U.S.A.

Summary

Different fractions of a mixture of protein-pigment complexes have been separated
from one another by non-denaturing gel electrophoresis. These complexes were
prepared for observation by inserting electron microscope grids directly into the
focused bands of the pigment-protein complexes and resuming electrophoresis for a
brief time, so that the complexes were deposited onto the grids. It was found that
complexes deposited from each band exhibited distinctly different appearances. It was
also found that the exact conditions of electrophoretic deposition onto the grids
affected the appearance of the complexes. The protein-pigment complexes were
characterized additionally by spectroscopy and denaturing gel electrophoresis.


Journal of Microscopy, Vol. 178, Pt. 2, May 1995, pp. 125-133

V(Z) curve formation of solid spherical microparticles in scanning acoustic
microscopy

K. I. Maslov,* P. V. Zinin, O. I. Lobkis* & T. Kundu
*Institute of Chemical Physics, Russian Academy of Sciences, Kosygin Str. 4, 117334
Moscow, Russia, Physics Department, Institute for Material Science and Structure
Research, University of Bremen, 28334 Bremen, Germany, Department of Civil
Engineering and Engineering Mechanics, University of Arizona, Tucson, AZ 85721,
U.S.A.

Summary
Information about the properties of materials in acoustic microscopy can be obtained
in the form of the V(Z) curves. The purpose of this paper is to present the theoretical
and experimental study of the V(Z) curve formation for solid spheres. It is shown that
an investigation of the position of different peaks in the V(Z) curves is useful to
determine the size and acoustical properties of a spherical particle.


Journal of Microscopy, Vol. 178, Pt. 2, May 1995, pp. 134-145

An ion microprobe study of the microchemistry of Ni-base superalloy-Al2O3
metal-matrix composites

K. K. Soni,* M. W. Tseng, D. B. Williams, J. M. Chabala,* Jianwei Li,* R. Levi-Setti*
& C. C. Bampton
*Enrico Fermi Institute and Department of Physics, The University of Chicago,
Chicago, IL 60637, U.S.A., Department of Materials Science and Engineering, Lehigh
University, Bethlehem, PA 18015, U.S.A., Rockwell International Science Center,
Thousand Oaks, CA 91358, U.S.A.

Summary
The Chemical microstructure of Ni-base superalloy/Al2O3 metal-matrix composites
(MMCs) has been studied by scanning ion microprobe microanalysis, using the
secondary ion mass spectrometry (SIMS) technique. The MMCs were fabricated using
the transient-liquid-phase bonding (TLP) process, with B-doped superalloy powder
as an interlayer. Boron was found to diffuse rapidly throughout the matrix to form
boride phases, mostly at the grain boundaries in the matrix. These borides contain
excess Cr (also Mo, Si, W) in comparison with the Ni alloy-matrix, but are depleted
in Ni (also in Al and Co). Carbides form at the grain boundaries as thin platelets and
inside the grains as fine particles. Chemical reaction occurs between the sapphire
fibre and the matrix; formation of NiAl2O4 spinel at the interface is suggested. This
interface reaction layer is friable and parts of it peel off during consolidation to become
inclusions in the matrix near the fibre/matrix interface.


Journal of Microscopy, Vol. 178, Pt. 2, May 1995, pp. 146-151

Estimation of individual feature surface area with the vertical spatial grid

L. M. Cruz-Orive* & C. V. Howard
*Stereology Unit, Department of Anatomy, University of Bern, Postfach 139, CH-3000
Bern 9, Switzerland, Department of Fetal and Infant Pathology, Royal Liverpool
Children's Hospital Alder Hey, Eaton Road, Liverpool L12 2AP, U.K.

Summary
The area of an individual bounded surface (e.g. the boundary of a properly sampled
cell) can be estimated from an isotropic uniform random stack of parallel sections, or
of non-invasive planar scans, using the well-known spatial grid. A standing problem
was to estimate the area of an individual bounded surface with an arbitrary degree of
accuracy from a vertical (i.e. not isotropic) stack of sections or scans. A new tool to
do this, called 'vertical spatial grid', is presented.



Journal of Microscopy, Vol. 178, Pt. 2, May 1995, pp. 152-159

Unbiased estimation of capillary length from vertical slices*

S. Batra, M. F K”nig & L. M. Cruz-Orive
Department of Anatomy, University of Bern, P.O. Box 139, CH-3000 Bern 9,
Switzerland

Summary
Previous stereological approaches to estimate feature length include isotropic sections,
which tend to be inefficient for highly anisotropic structures such as skeletal muscle
capillaries, and semiparametric model-based methods, which require transverse and
longitudinal sections only, but are biased to a variable, unknown degree. The recent
method of vertical slices combines the advantages of both approaches, namely it is
unbiased, efficient and convenient. This study illustrates for the first time how to apply
the vertical slices method in biology by direct light microscopy and intersection
counting with a properly orientated cycloid test system. Neither image processing nor
confocal microscopy are used. The purpose of the study was to estimate capillary
length in the left ventricle of rat heart. Beyond this, a novel histochemical method
enables the staining of the venular capillary region in red and the arteriolar capillary
region in blue, and hence estimates their separate lengths. The vertical slices method
to estimate feature length seems to be a promising approach for biology.

* Paper read at the Sixth European Congress for Stereology, Prague, 7-10 September
1993.


Journal of Microscopy, Vol. 178, Pt. 2, May 1995, pp. 160-164

Computer-assisted method for simultaneous three-dimensional reconstruction
of highly magnified nerve endings and low-magnification contours of th spinal
cord

A. G. Liss
Department of Anatomy, Uppsala University and Department of Plastic and Hand
Surgery, Uppsala University Hospital, Uppsala, Sweden

Summary
Three-dimensional reconstructions of biological structures can be obtained by the use
of serial sections, tomography, confocal microscopy techniques and X-ray
crystallography. The earliest reconstructions were achieved manually, but semi-
automatic and automatic techniques are now available. Tissue studied by microscopy
contains structures of greatly varying dimensions. When producing a three-
dimensional reconstruction of the contours of the spinal cord and its white and grey
matter, greater magnification of the fine nerve endings may provide additional
information. However, reconstruction at different magnifications of the structures of
interest cannot be achieved automatically, but requires manual delineation of the
structures. A method is described in which a commercial program was used to
provide a wire-frame reconstruction which had been drawn by hand. The data were
processed further to obtain a realistic image which could be rotated to provide details
of the three-dimensional relationships of the spinal cord structures. This techique is
useful when relationships and details are otherwise difficult to comprehend due to
large size differences.


Journal of Microscopy, Vol. 178, Pt. 2, May 1995, pp. 165-181

Restoration of confocal images for quantitative image analysis

H. T. M. Van Der Voort* & K. C. Strasters
*Department of Molecular Cell Biology, University of Amsterdam, Plantage
Muidergracht 14, 1018 TV Amsterdam, The Netherlands, Pattern Recognition Group,
Faculty of Applied Physics, Delft University of Technology, Lorentzweg 1, 2628 CJ
Delft, The Netherlands

Summary
Quantitative studies of three-dimensional (3-D) structure of microscopic objects have
been made possible through the introduction of microscopic volume imaging
techniques, most notably the confocal fluorescence microscope (CFM). Although the
CFM is a true volume imager, its specific imaging properties give rise to distortions in
the images and hamper subsequent quantitative analysis. Therefore, it is a
prerequisite that confocal images are restored prior to analysis. The distortions can
be divided into several categories: attenuation of areas in the image due to self-
absorption, bleaching effects, geometrical effects and distortions due to diffraction
effects. Of these, absorption and diffraction effects are the most important. This
paper describes a method aimed at the correction of diffraction-induced distortions.
All the steps necessary in restoring confocal images are discussed, including a novel
method to measure instrumental properties on a routine basis. To test the restoration
procedure an image of a fluorescent planar object was restored. The results show a
considerable improvement in the z-resolution and no ringing artefacts. The relevance
of the method for image analysis is demonstrated by a comparison of results of
applying 3-D texture analysis to restored and unrestored images of a synthetic object.
Furthermore, the method can be successfully applied to noisy fluores cence images
of biological objects, such as interphase cell nuclei.


Journal of Microscopy, Vol. 178, Pt. 2, May 1995, pp. 182-184
Short Technical Note
A system for aligning video-recorded visual data and high-quality multichannel
recordings of simultaneously occurring signals

T. Lumsdon, M. Dickson & M. H. Gladden
Institute of Physiology, University of Glasgow, Glasgow G12 8QQ, Scotland, U.K.

Summary
An electronic circuit is described which provides a unique number for each video
frame, and which is superimposed on the frame as a 4-digit light-emitting diode
display using a second camera and videomixer. The number is also encoded as a
simple voltage waveform which is recorded separately together with electrical signals
giving information about events related to, and coincident with, the visual data, for
example nerve spike trains. Sophisticated computer analysis of signals can thus be
related accurately to microscopical visual information processed by video analysis.


Journal of Microscopy, Vol. 178, Pt. 2, May 1995, pp. 185-187
Short Technical Note
A low-cost digitized microscope stage for linear measurements

B. H. Rohitha
The Horticulture and Food Research Institute of New Zealand Ltd, Ruakura Research
Centre, Private Bag 3123, Hamilton, New Zealand

Summary
A low-cost device for making fine, but repetitive linear measurements under the
binocular microscope is described. Linear displacement is coupled on to digital
callipers which read the measurements in numerical values. The accuracy of the
measurements was such that their 95% confidence interval was within 0.01 mm when
read through the apparatus. The data were assimilated on a computer and then
transmitted into a PC for analysis.




From: rms-at-vax.ox.ac.uk
Date: Fri, 30 Jun 1995 13:21:15 +0100
Subject: Still more J. Microsc. abstracts. Delete this now if unwanted!

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ABSTRACTS FOR MARCH AND APRIL 1995

Journal of Microscopy, Vol. 177, Pt. 3, March 1995, pp. 188-197.

Microscopy and formation of polymer/metal composites

D. Vesely, P. Disley & J. Pisacka Department of Materials Technology, Brunel University,
Uxbridge, Middlesex, UB8 3PH, U.K.

SUMMARY
A new class of materials, formed by dispersion of low-melting-point metal alloys in a polymer
matrix, has been studied from the point of view of microstructure, interfacial interaction and
mechanical properties. The phases in these composites were formed in the same way as for
polymer blends and were thus dependent on viscosity ratio, concentration, surface tension
and
interfacial interactions.
Metal alloys of tin and bismuth (Sn/Bi) were mixed with high-density polyethylene
(HDPE) and polystyrene (PS) at elevated temperatures. Some preliminary investigations of
lead and tin (Pb/Sn) alloys blended with HDPE, PS, polypropylene (PP), polyoxymethylene
(POM), polyethylene-terephtalate (PET), polymethylmethacrylate (PMMA) polycarbonate (PC),
polyvinylidenefluoride (PVDF) and polyvinylchloride (PVC) were also undertaken. The
composites were characterized by light and electron microscopy, image analysis, electrical
conductivity measurements and impact testing.
It is shown that the low-melting point metal alloys can be dispersed in polymers to a
submicrometre level by blending. The particle size distribution follows an exponential function,
which means that very fine as well as large particles are present. The equilibrium between
dispersion and coalescence is very rapidly established during mixing. The average particle
size can be controlled by the properties of the matrix, concentration of the metal and
processing conditions.
An investigation of interfaces revealed that in some cases a chemical interaction
between the metal and the polymer can occur. This is apparent by observation of
degradation, fluorescence and changes in mechanical properties.


Journal of Microscopy, Vol. 177, Pt. 3, March 1995, pp. 198-206

In-situ monitoring of fibre and matrix deformation in fibre-polymer composites

J.-P. Favre, M.-H. Auvray, P. Sigety, D. L‚vˆque & C. Brian‡on Materials Department,
Office National d'Etudes et Recherches A‚rospatiales (ONERA), BP 72, 92322-Ch…tillon
Cedex, France

SUMMARY
Three methods, namely microphotoelasticimetry, Raman spectroscopy and surface microgrids,
are currently used at ONERA on fibre-reinforced polymers to measure the in-situ fibre or
matrix deformation. They provide the materials scientist with valuable indications on the early
damage growth. Recent results are given and information about the limitations of the
methods
are indicated.


Journal of Microscopy, Vol. 177, Pt. 3, March 1995, pp. 207-217

The effect of microstructure on flow promotion in resin transfer moulding reinforcement
fabrics

P. R. Griffin, S. M. Grove, F. J. Guild, P. Russell & J. Summerscales School of
Manufacturing, Materials and Mechanical Engineering, University of Plymouth, Plymouth,
Devon PL4 8AA, U.K., Department of Materials Science and Engineering, University of
Surrey, Guildford, Surrey GU2 5XH, U.K. and Department of Biological Sciences, University
of Plymouth, Plymouth, Devon PL4 8AA, U.K.

SUMMARY
The resin transfer moulding (RTM) process is becoming increasingly important for the
manufacture of continuous fibre-reinforced thermosetting resin matrix composites. The RTM
process is a closed mould technique which reduces volatile emissions relative to traditional
hand lay-up methods. The fibres, generally as several layers of fabric, are prepared as a
preform and laid in the closed mould. The resin is injected, at one or more points, and flows
through the mould to form the finished product. In the manufacture of high-performance
composite structures, the flow of resin is constrained by the high volume fraction of
reinforcement fibres required to achieve the performance. Commercial fabrics are becoming
available which are woven with specially designed mesoscale architecture to promote flow of
the resin. The flow rates in a series of such fabrics have been studied. The microstructures
of the resulting composites have been examined using brightfield optical microscopy. A
Quantimet image analyser was used to quantify the structures on both the mesoscale and the
microscale. The flow rate has been shown to be related to the presence of both large and
more modest sized pore space in the reinforcement architecture.


Journal of Microscopy, Vol. 177, Pt. 3, March 1995, pp. 218-229

Influence of matrix precursors on the microstructure and mechanical properties of C/C
composites

A. Figueiras, J. J. Fern ndez, M. Granda, J. bermejo, E. Casal & R. Men‚ndez Instituto
Nacional del Carb¢n, CSIC, Apartado 73, 33080 Oviedo, Spain

SUMMARY
The microstructure and properties of uni-directional pitch-based carbon-carbon composites
are explained in terms of the chemical composition of pitch precursors. Pitches are
characterized by standard procedures (elemental analysis, softening point and solubility tests),
extrography which is a simple and rapid silica gel absorption chromatographic technique,
Fourier transform infra-red and gas chromatography of the toluene-soluble fraction. Pitch
pyrolysis behaviour is monitored by hot-stage microscopy. The main microstructural features
of uni-directional composites from pitches and commercial PAN-based carbon fibres are
determined by light microscopy and scanning electron microscopy.


Journal of Microscopy, Vol. 177, Pt. 3, March 1995, pp. 230-241

Microstructure and thermo-mechanical stability of a low-oxygen Nicalon fibre

M. H. Berger, N. Hochet & A. R. Bunsell Centre des Materiaux Pierre Marie Fourt, Ecole
Nationale des Mines de Paris, BP 87, 91003 Evry cedex, France

SUMMARY
A new Nicalon SiC-based fibre, characterized by a low oxygen content (0.5% wt) has been
studied. The absence in this fibre of a continuous Si-C-O phase, which characterized the
previous NLM 202 series of fibres, induces larger mean sizes for the constituents: the fibre
is composed of -SiC grains 5-20 nm in diameter and turbostratic aggregates of carbon 2-5
nm in diameter. The fibre is seen to be stiffer at room temperature (E = 300 GPa) and
stronger due to a reduction in critical defects thanks to improvements in processing conditions.

The Young's modulus remains almost stable up to 1473 K in air and above this temperature
the core of the fibre exhibits continuous grain growth up to 1773 K, but without the
degradation that occurred in the previous generation of fibres. Fibre strength was seen to be
lowered when compared to room temperature values even when exposed in air to
temperatures of 1073 K. A comparable fall is not seen with the NLM 202 fibres until 1273 K
and this difference is attributed to the oxidation of the carbon-rich surface of the new fibre.
SiC is oxidized at higher temperatures, inducing, above 1473 K, the growth of a silica layer
on the surface, with defects at the glass/ceramic interface. The large discrepancies between
the good thermo-mechanical characteristics in inert atmosphere and the behaviour in air may
be reduced if a coating resistant to oxidation could be applied to the fibre.


Journal of Microscopy, Vol. 177, Pt. 3, March 1995, pp. 242 -250

The microstructure of experimental SiC fibre-reinforced yttrium magnesium
aluminosilicate (SiCf-YMAS) materials

J. Vicens, F. Doreau & J. L. Chermant LERMAT, URA CNRS 1317, ISMRA, 6 Boulevard
du Mar‚chal Juin, 14050 Caen Cedex, France

SUMMARY
Two experimental SiC fibre-reinforced yttrium magnesium aluminosilicate (SiCf-YMAS)-type
ceramic-matrix composite (CMC) materials fabricated (i) by the glass process and (ii) by
chemical precursor infiltration have been studied by light microscopy, transmission electron
microscopy (TEM), high-resolution electron microscopy (HREM) and energy-dispersive X-ray
spectroscopy (EDS). The distribution of the fibres inside the composite as well as the
average
diameter of fibres have been determined by image analysis. The microstructure of the YMAS
matrices has been characterized by TEM observations. YMAS matrices are formed of two
main phases, cordierite and -yttrium silicate (Y2Si2O7). Two minor phases (mullite and
spinel) have been found to crystallize inside the cordierite and the yttrium silicate crystals.
Fibre-matrix interfaces have been observed in HREM. A thin turbostratic carbon layer (20-30
nm) has been imaged in both composites at the fibre-matrix interface. It crystallizes along
the matrix interface and grows inside the fibre, forming a diffuse interphase. The carbon layer
is believed to be the consequence of reaction between oxygen in the matrix and SiC
nanocrystals of the Nicalon fibres.



Journal of Microscopy, Vol. 177, Pt. 3, March 1995, pp. 251-272.

Environmental ageing effects in a silicon carbide fibre-reinforced glass-ceramic matrix
composite

K. P. Plucknett, S. Sutherland, A. M Daniel, R. L. Cain, G. West, D. M. R. Taplin & M.
H. Lewis School of Manufacturing, Materials and Mechanical Engineering, University of
Plymouth, Drake Circus, Plymouth PL4 8AA, U.K. and Centre for Advanced Materials,
University of Warwick, Coventry CV4 7AL, U.K.

SUMMARY
A silicon carbide fibre-reinforced glass-ceramic composite, based upon a BaO-MgO-Al2O3-
SiO2(BMAS) matrix, has been used for a study of microstructural stability (specifically
interface
stability) after environmental exposure at elevated temperature. Characterization of the as-
received material demonstrated the presence of a thin 'carbon-rich' interfacial layer between
fibre and matrix, as typically observed in glass-ceramic/silicon carbide fibre composite
systems. Samples have been subjected to heat-treatments in an oxidizing atmosphere at
temperatures between 723 and 1473 K, for up to 500 h. Intermediate-temperature ageing,
between 873 and 1073 K, results in strong fibre/matrix bonding, with consequent degradation
of strength and composite 'ductility'. This is due to oxidative removal of the carbon interfacial
layer and subsequent oxidation of the fibre surface, forming a silica bridge. Carbon is
retained
at higher ageing temperatures due to the formation of a protective surface oxide scale at
exposed fibre ends. Attempts to pretreat the BMAS composite at high temperature (1273 -
1473 K), designed to inhibit intermediate-temperature degradation via the formation of silica
plugs at exposed fibre ends, has given mixed results due to the high residual porosity content
in these materials, allowing paths of 'easy' oxygen ingress to be retained.


Journal of Microscopy, Vol. 177, Pt. 3, March 1995, pp. 272-278

Microstructure and evolution of a magnesium lithium aluminosilicate matrix composite

P. Ruterana, D. Kervadec, H. Maupas & J. L. Chermant LERMAT, URA CNRS 1317,
ISMRA, 6 Bd du Mar‚chal Juin, 14050 Caen Cedex, France

SUMMARY
The microstructure of a magnesium lithium aluminosilicate glass ceramic composite has been
investigated by scanning electron microscopy and analytical transmission electron microscopy.

Attention was focused on the as-received material, showing that there is a non-uniform
distribution of the major silicate phases inside the matrix. The largest part is made of
spodumene type crystals containing more than 4 wt% Mg. A minor part of the matrix is made
of micrometre-sized crystallites of spodumene and cordierite. The spodumene is always
sensitive to the electron beam irradiation. The morphology of the amorphized spodumene
areas indicates that it may have crystallized during a later stage of the matrix formation, filling
the gaps between cordierite crystallites. The third component of the matrix is made of
carbon-rich areas. They can be as large as 10 m and they always include amorphous Mg-
rich silicates. However, they are mainly small (a few tens of nanometres in width) when
located at grain boundaries of spodumene crystals. In this case the turbostratic carbon
patches are also intimately mixed with an Mg-rich amorphous silicate. The interface between
the matrix and the fibres has also been analysed, its thickness changes from one to the other,
and it is sometimes empty due to decohesion. When it is filled, its outer part contains mainly
tubostratic carbon and the inner part is a mixture of silicon oxide and probably carbon. After
creep at 1373 K, the spodumene-type crystals are larger and they are no longer sensitive to
the electron beam. The cordierite areas appear to shrink and the amorphous patches which
were mixed with carbon transform into small crystallites (1-10 m). The areas next to the
fibres are found to extend irregularly into the matrix, probably as a result of a chemical
reaction.


Journal of Microscopy, Vol. 177, Pt. 3, March 1995, pp. 279-286

Synthesis of silicon carbide ceramics from a polysilastyrene at low temperatures

S. M. McMillan & R. J. Brook Department of Materials, University of Oxford, Oxford OX1
3PH, U.K.

SUMMARY
The conventional route for preparation of silicon carbide ceramics is by the use of
pressureless sintering, hot pressing, or hot isostatic pressing of silicon carbide starting
powders. High sintering temperatures (2073-2473 K) and the addition of sintering additives
are normally used to enhance densification. These sintering additives, however, form second
phases at grain boundaries which impair the mechanical properties of the material, particularly
at high temperatures. It is therefore desirable that new processing routes are developed that
overcome these difficulties. A proposed route is to use a polymeric pressure which can
provide a silicon carbide matrix as binding agent for silicon carbide powders, thus making the
requirement for high temperatures and sintering additives unnecessary. This paper reports
observations of the direct transformation of a polymeric precursor into amorphous Si-C, and
crystalline SiC at low temperatures, and the use of this precursor as a binder for the
production of SiC powder/precursor SiC composites.


Journal of Microscopy, Vol. 177, Pt. 3, March 1995, pp. 287-304

A comparison of the microstructure of silicon nitride-silicon carbide composites made
with and without deoxidized starting material

S. Turan & K. M. Knowles University of Cambridge, Department of Materials Science and
Metallurgy, Pembroke Street, Cambridge CB2 3QZ, U.K.

SUMMARY
Two different types of silicon carbide (SiC) matrix composites, with either 10 wt% or 20 wt%
silicon nitride (Si3N4) reinforcement, were fabricated to investigate the effect of pretreatment
on the resulting composite microstructure. The first type of composite was prepared from as-
received à-SiC and à-Si3N4 powders, while the second type was prepared from powder
compacts that had been deoxidized to eliminate surface silica on the powder particles. The
composites were hot isostatically pressed in tantalum cans at 2373 K for 1 h under a pressure
of 200 MPa. Density measurements showed that full theoretical density was achieved for the
composites prepared from the as-received powders, while much lower densities were
obtained for the composites prepared from the deoxidized green compacts. Almost all of the
à-SiC transformed into -SiC, and almost all the à-Si3N4 transformed into -Si3N4 in the
composites made from the as-received powders, while in the composites made from the
deoxidized material the à-SiC remained untransformed and both à-Si3N4 and -Si3N4
phases
were present in significant quantities. High-resolution transmission electron microscopy and
Fresnel fringe imaging were used to identify the grain boundary and interphase boundary
structure. Most interfaces were found to be covered with 1nm thick amorphous intergranular
films in the composites prepared from as-received powders, whereas most interfaces were
found to be free of such amorphous intergranular films in the composites prepared from the
deoxidized material. Taken together, the presence of intergranular films at the interfaces and
the results from density measurements are consistent with the densification and reverse à
-SiC transformation taking place in the composites made from as-received powders by a
liquid-phase sintering route. An incomplete liquid-phase sintering mechanism is also able
to explain the microstructure observed in the composites made from the deoxidized material.


Journal of Microscopy, Vol. 177, Pt. 3, March 1995, pp. 305-312

Processing and properties of Al2O3/SiC nanocomposites

C. E. Borsa, S. Jiao, R. I. Todd & R. J. Brook Department of Materials, Oxford University,
Oxford, OX1 3PH, U.K.

SUMMARY
Alumina/SiC nanocomposites were produced by mechanical mixture of commercial powders.
The preparation steps involved the vigorous mixing of the powders and drying under
conditions where the homogeneous mixture was kept stable. Pressureless sintering of die-
pressed powders achieved reasonable densities ( 97% theoretical density) for 2.5wt% of SiC
on sintering at 2073 K. Higher SiC contents strongly reduced the sintered density. The use
of a more reactive alumina (finer matrix powder) gave similar results. Hot pressing at 1973
K/1 h/25 MPa produced high-density materials for SiC contents as high as 20 wt%.
Transmission and scanning electron microscopy analysis showed that the SiC particles were
well distributed and were situated both inside the grains and on the grain boundaries of the
alumina matrix. The SiC strongly inhibited grain growth in the matrix in keeping with the
Zener
model. The bend strength increased as the SiC content increased, a result partly explained
by the grain size refinement. The strength improvement of 20% over monolithic was
explained
in terms of the change to an intergranular fracture mode.



Journal of Microscopy, Vol. 177, Pt. 3, March 1995, pp. 313-319

Surface characteristics of Zirfon composite ultrafiltration membranes

S. Kuypers, I. Genn‚ & R. Leysen VITO (Flemish Institute for Technological Research),
Materials Division, Boeretang 200, B-2400 Mol, Belgium

SUMMARY
This paper presents the first high-resolution field emission scanning electron micrographs of
the skin layers of a series of Zirfon ultrafiltration membranes. The flux through these
polymer-based composite membranes is known to be proportional to the inorganic-filler
content. Image analysis of skin surface micrographs revealed the skin surface pore
characteristics to be almost unchanged over a wide range of compositions. The inorganic
filler
was found to be present in the skin layer, where it modifies the polymer network and locally
reduces the skin thickness. This latter effect might be able to account for the observed flux
behaviour.


Journal of Microscopy, Vol. 177, Pt. 3, March 1995, pp. 320-330

Interfacial reactions in PZT/Pd/PZT sandwich structures

L. G. Yao & R. J. Brook Department of Materials, University of Oxford, Parks Road, Oxford
OX1 3PH, U.K.

SUMMARY
The interfacial reactions of palladium foil and lead zirconate-titanate (PZT) were studied using
samples with a sandwich structure in the temperature range 1373 - 1523 K and under
conditions where no lead is lost to the environment. The interfacial reactions were analysed
using scanning electron microscopy, energy-dispersive X-ray spectroscopy, X-ray diffraction
and wavelength-dispersive X-ray spectroscopy analysis. The density of the PZT powder
phase increased with increasing temperature and, when sintered above 1373 K, reached over
95% of the theoretical density of PZT. The weight loss of pellets was less than 0.8% when
sintered below 1523 K. The degree of interfacial reactions became more severe with
increasing temperature, as indicated by an expanding reacted region. The reaction at the
PZT
side of the Pd/PZT interface involved the decomposition of PZT into a monoclinic ZrO2 phase,
PbO and a lower -value (Pb(Zr Ti1- )O3 composition. Three distinguishable microstructures
exist on the Pd side when sintered below 1473 K: a thin layer of PbPd3 phase, a Pd-Pb solid
solution zone and an unreacted region. Only the cubic PbPd3 eutectic structure was found
when sintered above 1473 K. The oxidation of palladium occurred during interfacial reactions,
expedited by increasing temperature and resulting in the formation of the tetragonal PdO
phase and the hexagonal PbPdO2 phase. A model for the overall reaction is proposed
involving decomposition of the PZT, migration of PbO and diffusion of Pb into Pd foil.



Journal of Microscopy, Vol. 177, Pt. 3, March 1995, pp. 331-336

Microstructural investigation of colloidal silver embedded in glass

H. Hoffmeister & M. Dubiel Max Planck Institute of Microstructure Physics, Weinberg 2, D-
06120 Halle (Saale), Germany and Martin Luther University, Department of Physics,
Friedermann-Bach-Platz 6, D-06108 Halle (Saale), Germany

SUMMARY
Nanoparticulate composites consisting of very small silver particles embedded in near-surface
regions of glass were obtained by sodium-silver ion exchange. Colloidal silver is formed by
reduction of silver ions and aggregation of silver atoms in the glass matrix at elevated
temperatures. Owing to absorption bands in the visible region, the silver particles cause a
coloration of the glass that depends on their size and depth distribution. From high-resolution
electron microscopy imaging of lattice plane fringes of the silver particles, size-dependent
lattice contractions are deduced that are larger than those reported in the literature for
supported particles not interacting with a matrix. This effect is the more pronounced the
higher the annealing temperature is during the particle formation. The increased lattice
contraction is attributed to compressive stresses that arise during the ion exchange process
as well as during cooling after annealing.



Journal of Microscopy, Vol. 177, Pt. 3, March 1995, pp. 337-346

Spray forming of Al/SiC metal matrix composites

P. S. Grant, I. T. H. Chang & B. Cantor Oxford Centre for Advanced Materials and
Composites, Department of Materials, University of Oxford, Parks Road, Oxford OX1 3PH,
U.K.

SUMMARY
This paper describes the as-sprayed microstructure of a model Al-4wt%Cu/SiC particulate
(Al4Cu/Sicp) metal matrix composite (MMC) manufactured by spray forming, and the
relationship between microstructure and solidification conditions during manufacture.
Injection of SiCp into the melt atomization region during the spray forming of Al4Cu
results in significant SiCp incorporation into molten droplets during atomization, and relatively
little incorporation during flight to the substrate and at deposition. SiCp clustering is evident
in the Al4Cu droplets and results in clustering in the as-sprayed MMC deposit.
Matrix dislocation and precipitation microstructures are dependent upon local
solidification conditions during spray forming. Increased dislocation density and increased
quantity of fine-scale '-Al2Cu precipitation is found in the à-Al(Cu) matrix where local
deposit
cooling rates are high, i.e. in the vicinity of the substrate/deposit interface and when increased
spray distances are used in manufacture. Lower dislocation density and increased quantity
of grain-boundary -Al2Cu is found where deposit cooling rates are relatively low, i.e. distant
from the substrate/deposit interface and at decreased spray distances. In all cases,
dislocation densities are higher in à-Al(Cu)/SiCp interfacial regions than in the à-Al(Cu)
matrix.
There is no evidence of à-Al(Cu)/SiCp interfacial reaction in the as-sprayed condition
indicating that cooling rates during spray forming are sufficiently rapid to prevent reaction.


Journal of Microscopy, Vol. 177, Pt. 3, March 1995, pp. 347-356.

Microstructural origins of corrosion in a 20% SiCp/2124 aluminium alloy metal matrix
composite

D. Imeson & D. L. Bartlett Structural Materials Centre, DRA Farnborough, Farnborough,
Hants. GU14 6TD, U.K.

SUMMARY
The microstructure of a metal matrix composite (MMC) consisting of 20 wt% 3 m SiC
particles
in a 2124 Al alloy matrix has been examined and the relationship to corrosion pitting
processes investigated. Standard bulk corrosion tests show that the MMC forms a higher
density of pits than the unreinforced alloy, although the overall performance is similar as the
pits are shallower. In a new addition to conventional characterization techniques, transmission
electron microscopy samples have been directly subjected to 'flash' corrosion treatment and
subsequently examined. This techique is shown to be effective in studying the initiation of
pits. The SiC particles and the widespread intermetallic precipitates are shown to play little,
if any, role. A sparse population of features introduced during powder processing and
consolidation procedures, probably linked with strong magnesium segregation, is suggested
to be responsible for pit initiation.


Journal of Microscopy, Vol. 177, Pt. 3, March 1995, pp. 357-368

Microstructure and fracture behaviour of particle-reinforced metal-matrix composites

B. Derby Oxford Centre for Advanced Materials and Composites, Department of Materials,
Oxford University, Parks Road, Oxford OX1 3PH, U.K.

SUMMARY
The fracture behaviour of particle-reinforced metal-matrix composites is shown to be
controlled by a period of damage nucleation and evolution prior to final failure. The nucleation
of damage can be by reinforcement fracture or decohesion and the mode of damage is shown
to be controlled by the size of the reinforcement and segregation of alloying elements from
the matrix. The nucleation and growth of damage can be monitored by a number of
techniques. Acoustic emission and tomography are used here and the results are found to
be consistent with simple models of void growth.


Journal of Microscopy, Vol. 177, Pt. 3, March 1995, pp. 369-386

Spatially resolved electron energy-loss studies of metal-ceramic interfaces in transition
metal/alumina cermets

R. Brydson, H. Mllejans, J. Bruley, P. A. Trusty, X. Sun, J. A. Yeomans & M. Rhle
Department of Materials Science and Engineering, University of Surrey, Guildford GU2 5XH,
Max-Planck-Institut fr Metallforschung, Institut fr Werkstoffwissenschaft, Seestrasse 92,
70174 Stuttgart, Germany and department of Materials Science and Engineering, Lehigh
University, Bethleham, PA 18015, U.S.A.

SUMMARY
Composites consisting of an alumina matrix and 20 vol.% transition metal (Ni or Fe) particles,
prepared by hot pressing powder blends, have been studied using spatially resolved
transmission electron energy-loss spectroscopy (EELS), and, to a lesser extent, by high-
resolution electron microscopy (HREM). Particular attention was paid to the elucidation of the
chemical bonding mechanisms at the metal-ceramic interface; EELS spectra from interfacial
regions being obtained via a spatial difference technique. From both qualitative and
quantitative interpretation of EELS near-edge structures, as well as observed HREM images,
the data appear to be consistent with the presence of an Al-terminated alumina at the
interface and the formation of direct transition metal - aluminium bonds in Al(O3M) (M = Ni
or Fe) tetrahedral units, possibly as a result of the dissolution and interfacial reprecipitation
of Al during processing. These results correlate well with similar model studies on diffusion-
bonded Nb/Al2O3 interfaces and may, in the light of recent theoretical electronic structure
calculations, have implications for the resultant interfacial bond strength in such materials.


Journal of Microscopy, Vol. 177, Pt. 3, March 1995, pp. 387 - 398

Microstructure of a spray-formed Al/SiC composite

P. Vermaut & P. Ruterana LERMAT, URA CNRS No. 1317, ISMRA, 6 Boulevard du
Mar‚chal Juin, 14050 Caen, Cedex, France

SUMMARY
The microstructure of an Al-6Cu-2Mn-0.45Mg-1(Ag,Ti,V,Zr,Cr) alloy, reinforced with 13 vol.%
SiC particles, made by spray deposition has been investigated by transmission electron
microscopy, high-resolution electron microscopy and electron diffraction X-ray spectroscopy.
Particular attention was focused on the influence of the reinforcement on the precipitation
sequence. Instead of the expected



precipitation sequence due to the high Cu/Mg ratio, there is an additional precipitate which
was previously observed in Al alloys containing silicon. This precipitate becomes predominant
at the T6 temper. The new precipitation sequence for this reinforced alloy is therefore




The precipitation of phase is believed to be due to the presence of SiC particles, and
seems to be correlated with the occurrence of large Mn-rich particles. Although expected,
no S phase precipitation is found to occur in the matrix grains. At the matrix grain boundaries,
small Al2Cu( ) and Al2CuMg (S), as well as Mn-rich precipitates are found. At the SiC
partical
surfaces, preferentially orientated Ag-rich and Mg-rich intermetallic precipitates are found.
They can coexist with amorphous patches containing oxygen enclosed in an irregularly
shaped
Al2Cu ( ) phase remaining from large crystalline areas which did not go into solution.




Journal of Microscopy, Vol. 177, Pt. 3, March 1995, pp. 399-406

X-ray microtomographic studies of metal matrix composites using laboratory X-ray
sources

P. M. Mummery, B. Derby, P. Anderson, G. R. Davis & J. C. Elliott Department of
Materials, University of Oxford, Parks Road, Oxford OX1 3PH, U.K. and Department of Child
Dental Health, London Hospital Medical College, Turner Street, London E1 2AD, U.K.

SUMMARY
X-ray microtomography (XMT) is a non-destructive technique that allows the internal
structure
of a material to be imaged by the spatial distribution of its linear X-ray absorption coefficients.

This paper demonstrates the use of XMT to investigate: (1) the distribution of TiB2
reinforcement in composites formed by powder processing; (2) the local void volume fraction
as a function of position in highly deformed regions of failed tensile specimens of SiC-
reinforced material allowing a valid damage parameter to be defined at high strains; (3)
absorption coefficients measured at different energies simultaneously using a multichannel
analyser which can sometimes be used to separate linear absorption changes due to (a)
density variations and (b) compositional variations in individual voxels; and (4) the use of
sequential sections to provide a three-dimensional representation of the failed specimens.


Journal of Microscopy, Vol. 177, Pt. 3, March 1995, pp. 407-413

The use of a single fibre test technique to investigate interfacial phenomena in SiC/Ti3
Al-based composites

F. Brisset & A. Vassel Direction des Mat‚riaux, ONERA, BP 72, 92322 Chƒtillon Cedex,
France

SUMMARY
A study of the chemical compatibility of a Ti3Al-based alloy (Ti-24A1-10Nb, at.%) with two
silicon carbide continuous fibres (SCS-6, SM 1240) has been conducted. Owing to the
difficulty in processing intermetallic matrix composites, this type of material has been
simulated in the present work by sputtering a thin titanium aluminide layer onto fibres and heat
treating at temperatures representative of fabrication conditions. The degradation of the fibre
strength due to its interaction with the matrix was correlated with analytical studies of the
fibre/matrix interface using a combination of SEM, TEM, EELS and a submicrometre ion
probe.


Journal of Microscopy, Vol. 177, Pt. 3, March 1995, pp. 414-423

Ion microprobe studies of reactions in squeeze-cast aluminium alloy matrix composites

K. K. Soni, H. G. Kang, P. S. Grant, B. Cantor, A. G. Adriaens, K. L. Gavrilov, R.
Mogilevsky, R. Levi-Setti, M. W. Tseng & D. B. Williams Enrico Fermi Institute and
Department of Physics, The University of Chicago, Chicago, IL 60637, U.S.A., Oxford Centre
for Advanced Materials and Composites, Department of Materials, University of Oxford, Oxford

OX1 3PH, U.K. and Department of Materials Science and Engineering, Lehigh University,
Bethlehem, PA 18015, U.S.A.

SUMMARY
An ion microprobe with high lateral resolution has been used to study the chemical reactions
at the fibre/matrix interface of metal-matrix composites. During the squeeze-casting
process,
the Al-Si-Mg matrix reacts with the preform made of Saffil fibres (96% Al2O3, 4% SiO2). The
reaction occurs mainly between the silica binder and Mg from the matrix according to SiO2 +
2Mg = 2MgO + Si. A continuous layer of MgO was formed around the fibres, even on
surfaces that were not covered by the silica binder. Possible reasons are discussed for the
formation of MgO in areas where binder coating was missing. In such areas, Mg reduces
SiO2 that is contained in the fibre. However, the fibres (Al2O3) are not attacked by Mg. In
the
isolated case of fibres that were completely uncoated, no reaction products were observed at
the interface. The presence of silica binder seems to be an essential requirement for this
reaction to occur. When squeeze-casting is performed with sufficiently high melt temperature,
Al from the matrix also reduces silica.



Journal of Microscopy, Vol. 178, Pt. 1, April 1995, pp. 1-6

Identification and surface structure of crystalline cellulose studied by atomic force
microscopy

L. Kuutti, J. Peltonen, J. Pere & O. Teleman VTT, Biotechnology and Food Research, PO
Box 1503, FIN-02044 Espoo, Finland and Department of Physical Chemistry, bo Akademi
University, Porthaninkatu 3-5, FIN-20500 Turku, Finland

SUMMARY
A combination of molecular modelling and atomic force microscopy (AFM) techniques was
used to study the surface structure of crystalline cellulose. Two-dimensional Fourier analysis
of the AFM raw data gave crystal parameters as well as a highly filtered inverse-transformed
image. Molecular modelling was used to generate Connolly surfaces based on electron
diffraction data for crystalline cellulose. The modelled surfaces were used to interpret the
experimental AFM images. Monoclinic (110) crystal faces were identified. The method used
enables the structural analysis of cellulose surfaces at the molecular level, where all biological
processes involving cellulose take place.



Journal of Microscopy, Vol. 178, Pt. 1, April 1995, pp. 7-13

Atomic force microscopy under liquid: a comparative study of three different AC mode
operations

T. M. H. Wong & P. Descouts Group of Applied Physics, University of Geneva, CH-1211
Geneva 4, Switzerland

SUMMARY
The image contrast mechanism of three newly proposed AC mode operations under liquid in
three different frequency ranges is presented. They all rely on a strong repulsive force to
damp the cantilever and the tip still 'touches' the sample surface. A direct comparison of the
three different modes of operation with the conventional DC mode technique using the same
gold sample under isopropanol was conducted. It was found that all the three AC modes
exerted a much smaller lateral force than the DC mode although the normal loads were of the
same order of magnitude. The suitability of such techniques in imaging physisorbed systems
on hard substrates (such as soft biological samples) and the prospect of a true non-contact
repulsive mode operation are discussed.


Journal of Microscopy, Vol. 178, Pt. 1, April 1995, pp. 14-19

A scanning near-field optical microscope having scanning electron tunnelling
microscope capability using a single metallic probe tip

Y. Inouye & S. Kawata Department of Applied Physics, Osaka University, Suita, Osaka 565,
Japan

SUMMARY
A new microscope system that has the combined capabilities of a scanning near-field optical
microscope (SNOM) and a scanning tunnelling microscope (STM) is described. This is
achieved with the use of a single metallic probe tip. The distance between the probe tip and
the sample surface is regulated by keeping the tunnelling current constant. In this mode of
operation, information about the optical properties of the sample, such as its refractive index
distribution and absorption characteristics, can be disassociated from the information
describing its surface structure. Details of the surface structure can be studied at resolutions
smaller than the illumination wavelength. The performance of the microscope is evaluated by
analysing a grating sample that was made by coating a glass substrate with gold. The results
are then compared with the corresponding SNOM and STM images of the grating.



Journal of Microscopy, Vol. 178, Pt. 1, April 1995, pp. 20-27

Three-dimensionally resolved NAD(P)H cellular metabolic redox imaging of the in situ
cornea with two-photon excitation laser scanning microscopy

D. W. Piston, B. R. Masters & W. W. Webb School of Applied and Engineering Physics,
Cornell University, Clark Hall, Ithaca, NY 14853, U.S.A., Department of Anatomy and Cell
Biology, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road,
Bethesda, MD 20814, U.S.A. and Department of Molecular Physiology and Biophysics,
Vanderbilt University Medical Center, 702 Light Hall, Nashville, TN37232, U.S.A.

SUMMARY
Three-dimensional maps of cellular metabolic oxidation/reduction states of rabbit cornea in
situ were obtained by imaging the fluorescence of the naturally occurring reduced pyridine
nucleotides (both reduced nicotinamide-adenine dinucleotide, NADH, and reduced
nicotinamide-adenine dinucleotide phosphate, NADPH, denoted here as NAD(P)H.
Autofluorescence images with submicrometre lateral resolution were obtained throughout the
entire 400 m thickness of the cornea. Two-photon excitation scanning laser microscopy with
near-infrared excitation provided high fluorescence collection efficiency, reduced
photodamage, and eliminated ultraviolet chromatic aberration, all of which have previously
degraded the visualization of pyridine nucleotide fluorescence. Sharp autofluorescence
images of the basal epithelium (40 m within the cornea) show substantial subcellular detail,
providing the ability to monitor autofluorescence intensity changes over time, which reflect
changes in oxidative metabolism and cellular dynamics necessary for maintenance of the
ocular surface. The autofluorescence was confirmed to be mostly of NAD(P)H origin by
cyanide exposure, which increased the fluorescence from all cell types in the cornea by about
a factor of two. Autofluorescence images of individual keratocytes in the stroma were
observed only after cyanide treatment, while in the predominant extracellular collagen (} 90%
of the stromal volume), fluorescence was not distinguished from the background. Observation
of keratocyte metabolism demonstrates the sensitivity made available by two-photon
microscopy for future redox fluorescence imaging of cellular metabolic states.


Journal of Microscopy, Vol. 178, Pt. 1, April 1995, pp. 28-36

A hybrid scanning force and light microscope for surface imaging and three-
dimensional optical sectioning in differential interference contrast

A. Stemmer Marine Biological Laboratory, Woods Hole, MA 02543, U.S.A.

SUMMARY
The design of a scanned-cantilever-type force microscope is presented which is fully
integrated into an inverted high-resolution video-enhanced light microscope. This set-up
allows us to acquire thin optical sections in differential interference contrast (DIC) or
polarization while the force microscope is in place. Such a hybrid microscope provides a
unique platform to study how cell surface properties determine, or are affected by, the three-
dimensional dynamic organization inside the living cell.
The hybrid microscope presented in this paper has proven reliable and versatile for
biological applications. It is the only instrument that can image a specimen by force
microscopy and high-power DIC without having either to translate the specimen or to remove
the force microscope. Adaptation of the design features could greatly enhance the suitability
of other force microscopes for biological work.



Journal of Microscopy, Vol. 178, Pt. 1, April 1995, pp. 37-41

Preliminary confocal scanning laser microscopy study of fluid inclusions in quartz

N. Petford, J. A. Miller & A. H. Rankin School of Geological Sciences, Kingston
University, Kingston KT1 2EE, U.K. School of Geological Sciences, Kingston University,
Kingston KT1 2EE, U.K. and Bullard Laboratories, Department of Earth Sciences, University
of cambridge, Madingley Road, Cambridge CB3 0EZ, U.K.

SUMMARY
The initial results of the first dedicated confocal scanning laser microscopy (CSLM) study of
fluid inclusions in quartz are presented. CSLM imaging of a large inclusion shows the quartz
crystal to contain numerous small ( {1 m), highly reflective inclusions arranged along planes
in at least two directions that are not readily visible in transmitted light. The technique allows
measurements to be made of the angular intersection and orientation of the planes in both
two
and three dimensions. Results suggest that larger inclusions (} 10 m) occur where two
planes of small inclusions intersect, and that the shape of the large inclusions is controlled by
the angular relationship between intersecting planes.



Journal of Microscopy, Vol. 178, Pt. 1, April 1995, pp. 42-47

STM imaging of metal-coated cell plugs of the archaeobacterium Methanospirillum
hungatei GP1

W. Xu, B. L. Blackford, P. J. Mulhern, M. H. Jericho, M. Firtel & T. J. Beveridge
Department of Physics, Dalhousie University, Halifax, Nova Scotia B3H 3J5, Canada and
Department of Microbiology, College of Biological Science, University of Guelph, Guelph,
Ontario, N1G 2W1, Canada

SUMMARY
Scanning tunnelling microscopy (STM) images of Pt/Ir- and Pt/Ir/C-coated cell plugs of
Methanospirillum hungatei showed paracrystalline structures with P6 symmetry and an 18-nm
lattice constant, in agreement with electron microscopy studies. The three-dimensional STM
images unambiguously distinguished the two morphologically different proteinaceous plug
assemblies and led to an improved understanding of the natural internal organization of whole
plugs. Tip convolution effects and the grain size of the metal coating complicated
interpretation of finer structures. We discuss possible imaging mechanisms to explain
observations in which part of the film was removed but the remaining part of the structure was
still imaged reproducibly.



Journal of Microscopy, Vol. 178, Pt. 1, April 1995, pp. 48-55

Maximum entropy reconstruction of compositional depth profiles from electron probe
microanalysis data

G. C. Smith, D. Park & O. Cochonneau Shell Research Ltd, Thornton Research Centre, PO
Box 1, Chester CH1 3SH, U.K. and Departement de Genie Mathematique, INSA de Rouen,
BP 08, 76131 Mont Saint Aignan, France

SUMMARY
Electron probe microanalysis (EPMA) is a powerful method for the quantitative determination
of the elemental composition of micro-regions of a sample surface. Here, we report on the
development of a method of reconstructing compositional depth profiles in thin films from
EPMA data measured over a range of electron beam energies, using maximum entropy data
processing. The method gives quantitative information on film compositions up to
approximately 1 m in depth, with a lateral spatial resolution of approximately 1 m. The
method is tested using both simulated data and measured experimental data from well-
characterized model sample structures.



Journal of Microscopy, Vol. 178, Pt. 1, April 1995, pp. 56-65

Monitoring cutting forces with an instrumented histological microtome

A. Willis & J. F. V. Vincent Centre for Biomimetics, The University, Reading, U.K.

SUMMARY
A modification to the knife mounting of a histological microtome is described which can sense
the load on the knife when a section is being cut. The performance of this microtome is
described in general terms. The variation in force on the knife can be used to give
information
about the texture of the sample being cut.



Journal of Microscopy, Vol. 178, Pt. 1, April 1995, pp. 66-85

The biological reality of the interlacunar network in the embryonic, cartilaginous,
skeleton: a thiazine dye/absolute ethanol/LR White resin protocol for visualizing the
network with minimal tissue shrinkage

D. M. Lawton, W. B. Oswald & J. McClure Department of Pathological Sciences, University
of Manchester, Stopford Building, Oxford Road, Manchester M13 9PT, U.K.

SUMMARY
Third toe phalanges of chicks aged 8-13 days in ovo and 7-day post-natal rat femoral growth
plate were examined to determine whether the interlacunar network (IN), a structure with no
lipoprotein membrane component or cytoplasmic organelles, is a genuine component of young
growth cartilage. In chick phalanges dehydrated by 70% (v/v) ethanol and LR White resin,
variable metachromatic staining of the interlacunar network by toluidine blue and red staining
by picro-Sirius red indicate the presence of glycosaminoglycans and collagen. The network
in phalanges dehydrated by 80% (v/v) ethanol apears little different; however, the network is
much less widely detectable in phalanges dehydrated by 90% (v/v) ethanol and, after
dehydration by absolute ethanol, is almost completely undetectable. In contrast, when the
young cartilage is permeated by a thiazine dye such as toluidine blue, using a solution of dye
in the aldehyde fixative, the network is widely detectable, following dehydration by absolute
ethanol, both in chick phalanges and in rat growth plate. Comparison of projected areas
shows that the extent to which whole chick feet are found to have shrunk, by the time that
they are photographed under LR White resin, is determined principally by the extent of
dehydration, by 70% (v/v) or absolute ethanol; post-shrinkage areas are 33% or 35% of areas
measured in buffer for 70% (v/v) ethanol/LR White resin and 71% or 75% for absolute
ethanol/LR White resin (the higher value in each is for the toluidine blue treatment). The
network is thus present in radically shrunk tissue, but, significantly, is also fully represented
in tissue shrunk by only a conventional margin and is therefore not produced as an artefact
by exceptional tissue shrinkage as has been suggested.



Journal of Microscopy, Vol. 178, Pt. 1, April 1995, pp. 86-92

Inherent bias in correlation averaged images

J. T. Woodward, C. Kono, L. L. Madsen & J. A. Zasadzinski Department of Physics,
Materials Research Laboratory, and Department of Chemical and Nuclear Engineering,
University of California, Santa Barbara, CA 93106, U.S.A.

SUMMARY
The correlation averaging algorithm frequently used to enhance micrographs of repeating
structures contains an inherent bias that favours images with larger pixel values or positive
noise levels. This bias not only skews the composite image toward higher pixel values, but
also distorts the image by increasing the value of high-valued pixels more than that of low-
valued pixels. These errors are especially important in scanning probe microscopy images
where the pixel value reflects a distinct height. A similar algorithm that uses a structure
function in place of the correlation function eliminates this bias.





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Date: Fri, 30 Jun 1995 13:16:19 -0500
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From: Krzysztof Hubner :      zahubner-at-cyf-kr.edu.pl
Date: Fri, 30 Jun 1995 17:27:08 +0200 (METDST)
Subject: short glass fibre composite

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Message-ID: {n1407615833.63628-at-qmgate.anl.gov}
"Stadden, David R" {DRSTADDE+aCENTRAL%Armstrong-at-mcimail.com}
X-Mailer: Mail*Link SMTP-QM 3.0.2

RE} FEG SEM for EDX? 6/30/95

Go for the FEG! The working distance is usually closer so you can do nice
imaging as well as EDS. The only limitation is the detector window material
and the Kv for the beam.
Obviously if you want to see sodium you need at least 1 kv, maybe 2kv to
excite the X-rays.

--------------------------------------
#012#
To: MICROSCO:CENTRAL
cc: DRStadden:R_D:Armstrong
Subj: FEG SEM for EDX?
------------------------------------------------------------------
A friend in academia is preparing to purchase an SEM to serve broad
interests in the sciences. He is wondering whether the field emission
gun instruments work as well for EDX of elements on the light side, such
as sodium and magnesium. Not too concerned with B,C,N,O and F. For
resolution and low KV work, he'd obviously like to have the FEG. Any
thoughts?

Dave Stadden

DRStadde+aCENTRAL%ARMSTRONG-at-MCIMAIL.COM
PH: 717-396-5109
FAX: 717-396-5865


------------------ RFC822 Header Follows ------------------
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Dear Friends

I have the following problem.
I was given some samples of a composite: short glass fibre -
transparent resin used as matrix.
I am supposed to examine under microscope the orientation of
these fibres.

The difference in grey level between the fibre and the resin is
very small; in practice only grey boundaries of the fibre - resin
system are visible.

The attempt to use various optical techniques (polarised light,
phase contrast etc.) to intensify the contrast between fibre and resin gave
a negative result. Etching in CrO2, HF was no good either.

And hence my question - maybe you now some
other means of preparation of the samples and of intensifying the
contrast between fibre and resin:
- chemical etching,
- image processing by means of mathematical morphology.

Thank you your kind reply.
Accept my best regards.


Krzysztof Jan Hubner {zahubner-at-cyf-kr.edu.pl}
Foundry Research Institute
Research Department - Structural and Physical Research Laboratory
Zakopianska 73 Call +48 12 605022 ext. 356
30-148 KRAKOW - POLAND Fax +48 12 665478 :-)






From: Richard Lee :      richard_lee-at-qmgate.anl.gov
Date: 30 Jun 1995 11:14:30 -0600
Subject: Re: FEG SEM for EDX?

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Message-ID: {n1407611436.28988-at-qmgate.anl.gov}
"Stadden, David R" {DRSTADDE+aCENTRAL%Armstrong-at-mcimail.com}
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From: Richard Lee :      richard_lee-at-qmgate.anl.gov
Date: 30 Jun 1995 10:00:27 -0600
Subject: Re: FEG SEM for EDX?

Contents Retrieved from Microscopy Listserver Archives
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RE} FEG SEM for EDX? 6/30/95

Go for the FEG! The working distance is usually closer so you can do nice
imaging as well as EDS. The only limitation is the detector window material
and the Kv for the beam.
Obviously if you want to see sodium you need at least 1 kv, maybe 2kv to
excite the X-rays.

--------------------------------------
#012#
To: MICROSCO:CENTRAL
cc: DRStadden:R_D:Armstrong
Subj: FEG SEM for EDX?
------------------------------------------------------------------
A friend in academia is preparing to purchase an SEM to serve broad
interests in the sciences. He is wondering whether the field emission
gun instruments work as well for EDX of elements on the light side, such
as sodium and magnesium. Not too concerned with B,C,N,O and F. For
resolution and low KV work, he'd obviously like to have the FEG. Any
thoughts?

Dave Stadden

DRStadde+aCENTRAL%ARMSTRONG-at-MCIMAIL.COM
PH: 717-396-5109
FAX: 717-396-5865


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From: Richard Crang :      rcrang-at-pop.life.uiuc.edu
Date: Thu, 29 Jun 1995 12:51:00 -0500
Subject: Containing Unicells for Freeze-Substitution

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microscopy studies and would like to have some of them prepared by means of
freeze substitution. They are in an osmotically adjusted buffer medium as a
cell suspension and, while we can centrifuge them (gently) to condense them
and even freeze them in chilled propane, they will disperse during the
substitution process, and particularly upon warming. Thus, they will be
lost upon the exchange of fluids. We are using a Reichert CS-Auto FS
system. Any suggestions how the material (or any unicellular preparations
for that matter) can be handled to contain them during freeze substitution?
Maintaining the cells on agar is not possible due to the need to keep them
in an osmotic medium prior to freezing. Suggestions greatly appreciated.



**************************
Richard F. E. Crang
Professor of Plant Biology
University of Illinois
(217) 244-3143
**************************





From: wise-at-vaxa.cis.uwosh.edu
Date: Fri, 30 Jun 1995 10:40:25 +0000
Subject: Yet more legal/ethical drivel

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There are several points I would like to add to the discussion
about unfairness of tax-supported (and tax-exempt) universities competing
against the heavily-taxed industrial sector.

--The tax breaks (or subsidies) given to the industrial sector since the
beginning of the Regan era have substantially shifted the tax burden from
businesses onto private citizens (mostly the middle class--where virtually
all university professors reside). The American business community has
never seen a more favorable tax or regulatory environment in the history of
our nation. The present Congress is charging ahead to further reduce taxes
and safety, health, environmental and fair business practice regulations as
they pertain to the industrial sector, thus further improving the business
environment. I submit that American business is not being taxed and
regulated to death.

--Public funding for universities has declined steadily for the last 20
years. Academic departments typically are only able to replace 1 (maybe
1.5) out of every three retirements. Faculty numbers are decreasing while
student enrollments stay the same or increase. A "four-year degree" takes
5 years at most schools because students, in spite of paying higher tuition
every year, cannot get into the classes they need for graduation. As an
example, look at how much public support the California public higher
education system enjoys from its citizenry. The University of Wisconsin
System will receive a 5.1% ($43 million) reduction in state moneys in the
next fiscal year. Pay raises for UW faculty (who are forbidden by state
law from unionizing) have been at or below inflation for most of the last
two decades. The notion of bottomless public coffers being used to drive
private industry out of business runs at odds with the current state of
financial support of public higher education in this country.

--My understanding is that most of the private EM testing labs that have
closed or down-sized recently are the result of a precipitous drop-off in
the asbestos testing business--not unfair competition from tax-supported
public universities.

--Dr. Garber's concern about the liability issue seems somewhat misplaced.
American universities are responsible for the health and safety of
literally millions of students and employees every day, any one of which
could sue the university at any time, for any reason. To state that "this
activity (product testing) puts the entire university at risk in ways that
are never even considered" and that "the real losers are the taxpayers, who
now have to carry the burden or risk of the folly of the commercial
activities being conducted out of the university's laboratories" implies
that this huge industry (i.e. public higher education), that predates the
founding of our country, has never faced a law suit. University
administrators are fully aware of the litigious society in which we live.
Hell, we train the lawyers!

--The UW Oshkosh Hitachi 2460N SEM with Noran Voyager EDS was paid for (in
total) by a gift from an anonymous, local industrialist. Does that exempt
us from NSF Notice 91 and those who interpret it to cover all
instrumentation housed at a public university (whether NSF-supported or
not)? What if our private benefactor asked for some free beam time (on the
machine he/she paid for)? Would I have to turn him/her away?

--In our situation, it was members of the private sector who came to us and
asked if they could rent our SEM (not the other way around). When we told
them "Yes, but at the same rate as you would have to pay a private lab"
they bolted, pulled an old SEM out of mothballs, and I haven't heard from
them since. They wanted access to a state-of-the-art SEM/EDS without
having to pay the going rate. Without the cooperation, and even
encouragement, of members of the private sector, universities would have no
market for the testing services which some would say unfairly compete
against other members of the private sector.

Let me close by saying that we provide no services at UW Oshkosh
that compete with the private sector. We did look into the possibility
once when specifically approached by industry, but have never pursued it of
our own volition. However, I do take a little umbrage at the notion that
fat university professors are using tax-payer's dollars to sweeten their
own nests while the tax-paying private sector carries the fiscal burden for
it all. It's a competitive world out there, guys. If you don't believe
me, apply for a tenure-track position somewhere.

Bob

Robert R. Wise, PhD
Director, UWO Electron Microscope Facility
Department of Biology
University of Wisconsin Oshkosh
Oshkosh, WI 54901
(414) 424-3404
(414) 424-1101 fax
wise-at-vaxa.cis.uwosh.edu






From: Christine.A.Snay-at-Dartmouth.EDU (Christine A. Snay)
Date: 30 Jun 95 15:05:00 EDT
Subject:

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From: AWBlackwoo-at-aol.com
Date: Fri, 30 Jun 1995 16:13:18 -0400
Subject: Glass Fiber Composites

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Dr. Krzysztof Hubner has raised some questions about examination of
transparent resin matrices reinforced with short glass fibers.

I must say that most of our experience has been with systems which produce
very good contrast under polarized light. They are not black/white
differences, however, they are shades of gray.

There is another approach to this problem which has produced very good
results, also. The use of an oxygen plasma will rapidy remove the matrix
while leaving the glass fibers untouched. Scanning electron microscopy can
then be used to image the fibers and their orientation, with as much contrast
as one might desire.

For this use, the nondirectional plasma of the barrel type of etcher would be
better than the directional plasma of the parallel plate type.

Yes, my employer, Structure Probe, sells both kinds of etchers through its
SPI Supplies Division, and further information on these units can be obtained
from SpiSupp-at-aol.com or by contacting me.

Andy Blackwood

Andrew W. Blackwood, Ph.D.
Structure Probe, Inc.
63 Unquowa Road
Fairfield, CT 06430-5015
Ph: 1 203 254 0000
FAX: 1 203 254 2262
e-mail: AWBlackwoo-at-aol.com





From: Christine.A.Snay-at-Dartmouth.EDU (Christine A. Snay)
Date: 30 Jun 95 16:19:21 EDT
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From: Michael Rock :      merock-at-u.washington.edu
Date: Fri, 30 Jun 1995 13:42:36 -0700 (PDT)
Subject: unsubscribe vacation

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From: bob-at-befvax.uchicago.edu
Date: Sat, 01 Jul 1995 11:34:34 EDT
Subject: Post doctoral Position

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POSTDOCTORAL POSITION AVAILABLE

A postdoctorial position is available for work on the
structure of fibers of sickle cell hemoglobin (HbS) and the
red cell cytoskeleton.

RED CELL CYTOSKELETON
Red cells are able to elastically deform. The
extraordinary importance of this property can be
appreciated from recalling that, although the
human red cell is a biconcave disk eight microns in
diameter, it can easily pass through capillaries
half this size. This remarkable feat is
accomplished by the red cell transiently changing
its shape during passage after which its well-
known biconcave form spontaneously and nearly
instantly recovers. We are studying the structural
basis of this property using cryoelectron
microscopy.

McGough, A. and Josephs, R. (1990) On the structure of
erythrocyte spectrin in partially expanded skeletons. Proc. Notl.
Acad. Sci. USA 87: 5208-5212.

Li Li and Robert Josephs. Cryo-Electron Microscopy of Human
Erythrocyte Membrane Skeletons. Proceedings of the 51st annual
meeting of the Microscopy Society of America San Francisco
Press, San Francisco. p. 116 (1993).

SICKLE CELL HEMOGLOBIN
Sickle cell anemia is caused by the
intracellular polymerization of sickle cell
hemoglobin to form rod-like fibers. A knowledge of
the fiber structure could be used for the design of
an agent that could block fiber formation. We have
combined the structure of hemoglobin (known from
X-ray crystallography) and the molecular
coordinates of the fiber (determined from electron
microscopy) in order to synthesize a model which
shows the intermolecular contacts of the fiber.
This approach has allowed us to determine the
contacts which form between molecules in the
fiber. We are now studying how various mutations
(some obtained by site directed mutagenesis)
affect fiber structure. Such studies are expected
to account for the structural and chemical
properties of fibers in terms of intermolecular
interactions.

Watowich, S., Gross L., and Josephs, R. (1989) Intermolecular
Contacts within Sickle Hemoglobin Fibers. J. Mol. Biol. 209:
821-828.

M. R. Lewis, L. J. Gross, and R. Josephs. Variable Pitch in Frozen
Hydrated Sickle Hemoglobin Fibers: An Image Analysis Model
Study. Ultramicroscopy, 56 303-317 (1994).

Michael R. Lewis, Leon J. Gross, and Robert Josephs. Cryo-
Electron Microscopy ofDeoxy-Sickle Hemoglobin Fibers.
Microscopy Research and Technique 27 459 - 467 (1994).


Interested individuals please contact
Robert Josephs
The University of Chicago
920 East 58th Street
Chicago, IL 60637
E-Mail Bob-at-befvax.Uchicago.Edu





From: John Millar :      jjmill-at-RMIT.EDU.AU
Date: Mon, 3 Jul 1995 09:03:50 EST-10
Subject: addresses

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Dear List
Can anyone supply the addresses of

Dr. P.Mummery from materials at Oxford , UK
Dr. G. Smith from Shell Research Labs , Chester, UK

Thank you
jjm
Professor John J. Millar
Head, Department of Applied Physics
Electron Microscope Unit
RMIT, GPO Box 2476V, MELBOURNE AUSTRALIA 3001
+613 660 2602 fax 613 660 3837
email jjmill-at-rmit.edu.au




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Date: Sun, 2 Jul 1995 18:33:24 -0700
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Date: Mon, 3 Jul 1995 07:32:28 +0200
Subject: cancellation of subscription request

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Please cancel my subscription because I have got any information.
I have only a lot of message about delivery problems.
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Date: Mon, 3 Jul 1995 17:00:16 -0700
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From: Clint E. Young :      clintey-at-unixg.ubc.ca
Date: Mon, 3 Jul 1995 06:16:55 -0700 (PDT)
Subject: Q-IA: RESULTS MAILING LIST

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b2YgUHN5Y2hpYXRyeQ0KVW5pdmVyc2l0eSBvZiBCcml0aXNoIENvbHVtYmlh
DQo=
---559023410-851401618-804777415=:21247--




From: Clint E. Young :      clintey-at-unixg.ubc.ca
Date: Mon, 3 Jul 1995 06:28:02 -0700 (PDT)
Subject: Q-IA: RESULTS MAILING LIST

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To those who participated,

The following contains the compiled information that participants sent me
regarding a Quantitative Image Analysis (Q-IA) mailing list.

It is divided into 4 sections.
1. ID#: Name and email address
2. Questions and comments made by participants
3. Q-IA that participants are currently ABLE to perform.
4. Computer and Software used

Immediately afterwards is my suggestion of where a (temporary?) Q-IA
mailing list should be--and my reasons for choosing this site.

SECTION 1: NAME & EMAIL ADDRESS
--------------------------------

ID# Name e-mail
1 ? smejkag-at-ccsmtp.ccf.org
2 Alan Hall hall-at-scientia.up.ac.za
3 Alex Almasan almasaa-at-athene.hh.ri.ccf.org
4 Alistair Young a.young-at-auckland.ac.nz
5 Andreas Becker becker-at-ps1515.chemie.uni-marburg.de
6 Bo Johansen boj-at-bot.ku.dk
7 Brain Wade bwade-at-ux1.cso.uiuc.edu
8 Carolyn Smith clsmith-at-codon.nih.gov
9 Chang Seng chang-at-newton.umsl.edu
10 Cheng-Lun Na na-at-utsw.swmed.edu
11 Craig Daly cdaly-at-biomed.gla.ac.uk
12 David A. Jans daj224-at-huxley.anu.edu.au
13 David boyle david.boyle-at-gensia.com
14 David Morilak morilak-at-uthscsa.edu
15 Dominique Bataille Bataille-at-citi2.fr
16 Don-Carlos Abrams don-at-rpms.ac.uk
17 Donna Wagahoff DWAGAHOF-at-wpsmtp.siumed.edu
18 Doug Chaytor doug.chaytor-at-dal.ca
19 Douglas L. Rosene drosene-at-bu.edu
20 Edgar Riverdal edgarr-at-labmed.uio.no
21 Eric Kokko KOKKO-at-EM.AGR.CA
22 Feona Hansen-Smith hansensm-at-oakland.edu
23 Gary Krichau gkrichau-at-unlgrad1.unl.edu
24 Gert van Cappellen vanCappellen-at-endov.fgg.eur.nl
25 Glenn Holm karuzis-at-wccf.mit.edu
26 Gordon Robertson robertson-g-at-vanlab.paprican.ca
27 Hakan Hall hakanh-at-psyk.ks.se
28 Jakob Walter jhwpatho-at-zedat.fu-berlin.de
29 James Crandall jcrandall-at-shriver.org
30 Jeff Reece reece-at-niehs.nih.gov
31 Jeffrey Kopp jbkopp-at-nih.gov
32 John F Smiley JSmiley-at-nwu.edu
33 Judy Trogadis judy-at-camtwh.eric.on.ca
34 Kate Whitley k.whitley-at-ucl.ac.uk
35 Lauran Oomen lauran-at-nki.nl
36 Marc Brande brande-at-SDSC.EDU
37 Maxx Abraham abraham-at-atax.eng.uab.edu
38 Michael Binks reba999-at-ucl.ac.uk
39 Michael J. Herron herro001-at-maroon.tc.umn.edu
40 Michael Rudisill mrudisil-at-hsc.usc.edu
41 Mike Esteman mikee-at-lilly.com
42 Norm Granholm Norman.Granholm-at-UC.Edu
43 Paul C. Dolber dolber-at-cs.duke.edu
44 Paul Goodwin pgood-at-fred.fhcrc.org
45 Petra Nederlof nederlof-at-genmic.biochem.mpg.de
46 R. Thomas Zoeller tzoeller-at-bio.umass.edu
47 R. Wade Schuette schuette-at-eecs.umich.edu
48 Richard Thrift RichardT-at-Depotech.com
49 Rosemary White r.g.white-at-sci.monash.edu.au
50 Stephen Helfer s.helfer-at-rbge.org.uk
51 Steve Rogers srogers-at-delphi.beckman.uiuc.edu
52 Steven Bagley steven.bagley-at-man.ac.uk
53 Ted Gaten gat-at-leicester.ac.uk
54 Vlasta P Spongr spongr-at-acsu.buffalo.edu
55 Ze'ev Silverman szeev-at-bgumail.bgu.ac.il

SECTION 2: QUESTIONS/COMMENTS ASKED
--------------------------------------

ID# Name question
1 ?
2 Alan Hall ? how quantify RNA from material embedded in
Quetol with oso4?
3 Alex Almasan
4 Alistair Young ?what's good embedding compoud for confocal } 100um?
5 Andreas Becker quantitative PCR techniques
6 Bo Johansen
7 Brain Wade interest: quantify chlorophyll
8 Carolyn Smith
9 Chang Seng Semper6 plus is programmable IA software
10 Cheng-Lun Na ? Any EM autoradiography analysis software for Mac?
11 Craig Daly 3D of blood vessels; receptor distribution
12 David A. Jans interest: quantify flourescent labelled nuclear protein
13 David boyle Quantified radiolabelled tissue
14 David Morilak
15 Dominique Bataille ? how quantify stained antigen?
16 Don-Carlos Abrams
17 Donna Wagahoff
18 Doug Chaytor
19 Douglas L. Rosene ?how create standards for absolute IHC quantification?
20 Edgar Riverdal ? how quanify flourescent staining?
21 Eric Kokko
22 Feona Hansen-Smith
23 Gary Krichau
24 Gert van Cappellen ?how quantify IHC and in-situ hybridization?
25 Glenn Holm autoradiograms; cell counting and classification
26 Gordon Robertson
27 Hakan Hall autoradiograms; receptor distribution and densities
28 Jakob Walter ?macro for count labelled cells? ?est. MW with NIH?
29 James Crandall
30 Jeff Reece interest: [Ca++] in live cells
31 Jeffrey Kopp ? what microscope and CCD camera to buy?
32 John F Smiley quanitify IHC, 2 approaches: area stained; line intercept
33 Judy Trogadis recently published quantitative flourescence of receptors
34 Kate Whitley ? how quantify flourescent staining?
35 Lauran Oomen FISH analysis; enzyme levels
36 Marc Brande
37 Maxx Abraham Goal: automated flourescence immunoassay system
38 Michael Binks
39 Michael J. Herron
40 Michael Rudisill ? how quantify flourescence?
41 Mike Esteman
42 Norm Granholm ?advice on software?
43 Paul C. Dolber ? comments on background subtraction and calibration?
44 Paul Goodwin
45 Petra Nederlof ? how quantify flourescence?
46 R. Thomas Zoeller ? is computer enhancement of a signal legitimate?
47 R. Wade Schuette
48 Richard Thrift Interest: quantify hydrogen ions
49 Rosemary White ? how best quantify WB?
50 Stephen Helfer cell (fungal/plant) measurments
51 Steve Rogers
52 Steven Bagley
53 Ted Gaten ? how quantify dot blots?
54 Vlasta P Spongr
55 Ze'ev Silverman ?how quantify silver grains over cells?

SECTION 3: SUCCESSFUL Q-IA
---------------------------

IHC: immunohistochemistry
WB: western blotting

A. MACROSCOPIC FIELD
1. Number of cells stained
2. Size of cells
3. Distribution of cells

B. MICROSCOPIC FIELD
1. Amount of antigen present
2. Distribution of antigen
3. Colocalization of different antigens (confocal)


ID# ability
24 B(3)
43 Feulgen-DNA microdensitometry
29 IHC: A (1,2,3) + B(1,2)
33 IHC: A(1,2) + B(1,2,3)
47 IHC: A(1,2,3)
50 IHC: A(1,2,3)
10 IHC: A(1,2,3) + B(1,2)
21 IHC: A(1,2,3) + B(1,2)
32 IHC: A(1,2,3) + B(1,2)
49 IHC: A(1,2,3) + B(1,2)
6 IHC: A(1,2,3) + B(1,2,3)
19 IHC: A(1,2,3) + B(1,2,3)
25 IHC: A(1,2,3) + B(1,2,3)
35 IHC: A(1,2,3) + B(1,2,3)
39 IHC: A(1,2,3) + B(1,2,3)
44 IHC: A(1,2,3) + B(1,2,3)
54 IHC: A(1,2,3) + B(1,2,3)
7 IHC: A(1,2,3) + B(2)
18 IHC: B(1,2)
13 IHC:A(1,3) + B(1,2,3)
37 IHC; laeter, ISH
46 ISH, IHC, WB
30 ROI intensities vs. time
23 Shape Analysis: 2D
11 Shape Analysis: 2D + 3D
52 Shape Analysis: 2D + 3D
36 Shape Analysis: 3D
40 Simple, Particle analysis
1 WB: (1,2)
15 WB: (1,2)
20 WB: (1,2)
41 WB: (1,2)
53 WB:(1,2)
2
3
4
5
8
9
12
14
16
17
22
26
27
28
31
34
38
42
45
48
51
55

SECTION 4: COMPUTER & SOFTWARE USED
--------------------------------------

ID# computer software
1 Mac NIH
10 Mac NIH
12 Mac NIH
15 Mac NIH
18 Mac NIH
20 Mac NIH
23 Mac NIH
27 Mac NIH
28 Mac NIH
31 Mac NIH
32 Mac NIH
38 Mac NIH
39 Mac NIH
41 Mac NIH, IPLab, Prism; Image Pro Plus
43 Mac NIH
46 Mac NIH; BioQuant
51 Mac NIH; Photoshop, Analyze
53 Mac NIH
55 Mac NIH
5 Mac, PC NIH
6 Mac, PC NIH, Image Pro Plus, Image-1
7 Mac, PC NIH, Inspector
8 Mac, PC NIH; Image 1
13 Mac, PC NIH; V150
19 Mac, PC NIH; Inquiry, Bioquant
24 Mac, PC ImageQuant; Zeiss CLSM
34 Mac, PC NIH, Photoshop; Foster Findlay's PCImage
40 Mac, PC NIH, Photoshop, Zeiss CLSM, MathLAB
11 Mac, PC, SGI NIH, MetaMorph, Imaris & MicroVision
30 Mac, PC, SGI NIH, MetaFluor, Zeiss CLSM, VoxBlast, AVS
35 Mac, PC, SGI NIH, SCIL-Image, Photoshop
49 Mac, PC, SGI Own
52 Mac, PC, SGI NIH, PC Image, Semper,AVS
9 Mac, PC, SGI, Sun Semper6 Plus
37 Mac, PC, SGI, Sun NIH; Matlab, PV-Wave, Image Analyst
44 Mac, PC, SGI, Sun NIH, OPTIMAS, ImageQuant, ISee, Prism
16 Mac, PC, Sun Kontron, ContextVision +own
4 Mac, SGI NIH, Voxelview, Photoshop, 3DVIEWNIX
36 Mac, SGI NIH
45 Mac, SGI NIH +others
47 Mac, Sun NIH, Khoros, MATLAB, IMagist-II
17 PC Bioquant and MCID
22 PC Optimas
25 PC Biocom (French commerical software)
29 PC Eutectics NTS; IBAS
48 PC Optimas 4.0
50 PC DataCell OPTIMAS
54 PC Photoshop
33 PC, SGI, NextStep BioRad confocal software, ICAR (3D)
21 PC, Unix Visilog, PCI2
26 IBAS
2
3
14
42

--------------------

From SECTION 4, most if not all participants with Macintosh computers are
using NIH Image software (some in combination with Photoshop), while
participants using PC or Unix (SGI or SUN) stations do not seem to share
a common software package. It seems apparent that most participants
(42/55) are familiar with NIH Image. [Admittedly, these results may be
biased since I could not find a mailing list for SGI or SUN users doing
imaging. If you know of one, please let me know.]

For this reason, I suggest that we use the NIH Image mailing list as ONE
centre for Q-IA discussion. If a PC or UNIX mailing list performing
image analysis exists, I would certainly join in.

To subscribe to the NIH Image mailing list:

send an email message to

listserv-at-soils.umn.edu

with a message containing

"subscribe nih-image FIRSTNAME LASTNAME"

Obviously, there are many experienced and knowledgable people that have
been successful in certain areas of quantitative image analysis as
evidenced by this impromptu survey. I hope your questions can be
answered now that you know where (and who) to ask.

By the way, may I suggest that if you are asking a question regarding
quantitative image analysis, that you put "Q-IA: ..." as part of the
subject. And, finally, I am trying (if time permits) to create a Q-IA
Web Page. If you have any suggestions as to what I should put on it,
feel free to email me directly.

Looking forward to reading your comments.


Clint Young
Department of Psychiatry
University of British Columbia




From: NICK SCHRYVERS :      nick_schryvers-at-ruca.ua.ac.be
Date: 3 Jul 1995 15:40:06 +0100
Subject: Belgian Soc. Micr.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Message-Id: {n1407336050.86487-at-ematserv.ruca.ua.ac.be}

REGARDING Belgian Soc. Micr.

The Belgian Society for Microscopy is rapidly expanding. In order to
reestablish contacts with fellow countrymen and -women abroad, we kindly ask
all Belgians who are doing research in the field of any microscopy technique
(EM, AFM, STM, NFOM, AM, IRM, NMRM, .....) to leave their coordinates with us
so we can inform you of the activities of our society. In Septemeber 1995 a
leaflet including a return slip for membership will be mailed to everyone in
our files.
I hope te hear from you soon,

Nick Schryvers
co-secretary BVM-SBM
RUCA, University of Antwerp
Groenenborgerlaan 171
B-2020 Antwerp
Belgium
tel: 32-3-2180247
fax: 32-3-2180257
e-mail: schryver-at-ruca.ua.ac.be






From: yang-at-snmail.jsc.nasa.gov
Date: 6/28/95 12:27 PM
Subject: EPMA Beam deflection

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



EPMA=Jeol 733; 15Kv; probe curr 200x10E-07A.
While doing spot (2um) analysis on a metal alloy Fe, Ti, C and O it
ocurred
that the electron beam did not hit the target beneath the optical cross
of
the binoculars. It hit a point about -70um on the x-axis, i.e. to the
left of the cross.
Double CHECKING the probe's alighnment on ZrO2 fluorescent standard
confirmed
nothing wrong there, and the spot precisely beneath the cross. Focussing
on
a fine crack of the brass sampleholder showed a drop in Cu-counts just
when
the crack was beneath the cross; spot still beneath the optical cross.

The sample was earthed with, maybe a bit excessive amount, of SILVER DAG
to the sample holder. Watching simultaniously two ratemeters monitoring
Cu and Ag, it was evident that the SILVER DAG DEFLECTS THE BEAM AWAY FROM
THE SILVER MASS. Checking with a different spot af silver, one can
deduct
that the amount of deflection relates directly to the amount of silver.
The larger the silver mass the greater is the deflection. The silver
dag in question is not from my usual supplier, and not my brand of
colloidal silver I am normaly using.

CAN ANYONE PLEASE HELP EXPLAINING THIS PHENOMENON. Thanks. Leon.

} \\\\\\\\\\\\\\\\\\\\\} WWW: http://www.puk.ac.za} \\\\\\\\\\\\\\\\\\\\\\}
} Leon Smuts-Electronmicroprobe-University of Potchefstroom-South Africa}
} ///////////////////} e-MAIL: plbls-at-puknet.puk.ac.za} ///////////////////}








From: Georges_Veilleux-at-INRS-ENER.UQuebec.CA (Georges Veilleux)
Date: Mon, 3 Jul 1995 11:36:47 -0400
Subject:

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Microscopists,
}
} } From what source might we be able to obtain some crystalline graphite, in
} order to prepare a thin graphite support film, as described in an article in
} _Micron_, 1977, vol. 8:41-46 by Sumio Iijima?
}
} We've tried Ted Pella, SPI, Ernest Fullam, and Union Carbide. No luck.
}
} Any leads would be appreciated,
}
} Yolande Berta
} School of Materials Science and Engineering
} Georgia Tech
} yolande.berta-at-mse.gatech.edu
}
}

You may try:

Sigri Great Lakes Carbon Corp., Specialty Graphite

Charlotte, NC 28256 USA
800-828-6601 _Call Toll Free. (Sales)
704-437-3280 _FAX. (Sales)



Carbone Of America Ultra Carbon Div.

Bay City, MI 48708 USA
800-248-8218 _Call Toll Free.
517-895-7740 _FAX

To all the microscopy list:

One great place to search on the Internet is to register to the Thomas
Register of American Manufacturers at http://www.thomasregister.com/

After registration, it's free to use the "Supplier Finder" to search for
products or for company names
Georges Veilleux
Researcher
INRS-Energie et materiaux
C.P. 1020
Varennes, Qc
Canada
J3X 1S2

Tel.: (514) 929-8110
Fax: (514) 929-8102





From: a-davis-at-uchicago.edu (Andrew M. Davis)
Date: Mon, 3 Jul 1995 11:00:51 -0600
Subject: JEOL JSM-35 looking for a home

Contents Retrieved from Microscopy Listserver Archives
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X-Sender: ad15-at-midway.uchicago.edu
Message-Id: {v01510101ac1dd22ba7c8-at-[128.135.28.29]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

The University of Chicago is looking for a new home for the following SEM:
a JEOL JSM-35U, equipped with a two-element annular solid state
backscattered electron detector, a beam current stabilizer, a liquid
nitrogen baffle, a liquid nitrogen cold finger and a Kevex 7077 (PDP 11-23)
x-ray microanalysis system with a horizontal-entry (motor-driven) Si-Li
detector with a Be window. The SEM vacuum system has been upgraded with two
Edwards E2M8 direct drive rotary pumps and an Edward Diffstak diffusion
pump. The SEM and EDS system are in good condition and the photo CRT was
replaced two years ago. The University expects some cost recovery to aid in
acquisition of a new SEM-EDS system. If interested, please contact directly
(i.e., do not send a message to the Microscopy Listserver!):

Andrew M. Davis
Enrico Fermi Institute
University of Chicago
5640 South Ellis Avenue
Chicago, IL 60637
312-702-8164
312-702-9505 fax
a-davis-at-uchicago.edu






From: Dr P. Echlin :      pe13-at-cus.cam.ac.uk
Date: Wed, 5 Jul 1995 08:30:22 +0100 (BST)
Subject: BBSRC Studentship in Analytical Microscopy

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Message-Id: {9507041221.AA31752-at-pukrs7.puk.ac.za}


The Department of Plant Sciences, University of Cambridge has been
awarded a BBSRC Case studenship starting in October 1995 which will be
co-directed with Dalgety plc Food Technology Centre in Cambridge. Dalgety
plc is a major international company wide wide interests in food and
agriculture. Thge studentship will centre on the analytical microscopy
and biochemistry of the structure and cellular location of soluble and
non-soluble non-starch polysaccharides in cereals and pulses in relation
to their role in the processing of food stuffs. Candidates holding or
expecting a first or upper second degree in a relevant subject should
apply as soon as possible to Patrick Echlin Director Multi-Imaging
Centre, Department of Plant Sciences, University of Cambridge, Downing
Street Cambridge CB2 3EA UK. Tel; +44-1223-333946. Fax;
+44-1223-333953.Send CV and names of two academic referees. Successful
candidates will receive an additional payment of #2250 per annum and will
be elible to participate in Dalgety's internal management training
programme. Closing date for applications is July 20 1995, and interiews
are scheduled for July 24/25. The Multi-Imaging Centre has unparalleled
facilities for coherent light and high energy beam microscopy and in situ
analysis. If you are a biochemist, a food scientist of a plant scientist,
we can teach you the analytical microscopy.

Patrick Echlin
Director, Multi-Imaging Centre
School of Biological Sciences
University of Cambridge




From: na-at-UTSW.SWMED.EDU (Cheng-Lun Na)
Date: Wed, 05 Jul 1995 13:34:06 -0600
Subject: EM autoradiography analysis software for Mac

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Hello Imagers:

Does anyone know if there is any EM autoradiography analysis software
available for Macintosh? Any comment is welcomed.

Cheng-Lun Na
Cell Biology and Neuroscience
UT Southwestern Medical Center at Dallas
5323 Harry Hines Blvd.
Dallas, TX 75235-9039
PH: (214) 648-3597 (214) 648-2032
E-Mail: na-at-utsw.swmed.edu

Cheng-Lun Na
Cell Biology and Neuroscience
UT Southwestern Medical Center at Dallas
5323 Harry Hines Blvd.
Dallas, TX 75235-9039
PH: (214) 648-3597 (214) 648-2032
E-Mail: na-at-utsw.swmed.edu






From: JOHNA-at-SCI.WFEB.EDU
Date: Wed, 05 Jul 1995 15:43:11 -0400 (EDT)
Subject: Negative scanning & printing

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Hello Folks,

I am in the initial stages of gathering information on TEM and SEM negative
scanning/digitization and the subsequent printing of these images. I'd
appreciate receiving your thoughts on how best to approach these topics,
who manufactures such scanners, which are best for EM negs, which software
should be considered, should I be considering a souped up laser printer or
some other type (dye sublimation, etc.).

Any input regarding these topics would be quite helpful and greatly
appreciated. Thanks in advance.

John

___________________________________________________
| |
| John G. Aghajanian, Ph.D. |
| Worcester Foundation for Experimental Biology |
| 222 Maple Avenue |
| Shrewsbury, MA 01545-2737 |
| |
| Tel: 508 842-8921 ext. 147, 161 |
| Fax: 598 842-9632 |
| JOHNA-at-sci.wfeb.edu |
| |
|_________________________________________________|





From: James R. Stets :      stetsjr-at-ttown.apci.com
Date: Wed, 5 Jul 1995 20:27:10 -0400 (EDT)
Subject: Re: Digital Image Transfer from JSM-5400

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In response to Frank Wubbolts' question regarding transfer of the frame
store image from a JEOL JSM-5400 to a PC:

Yes, this can be done, assuming the 5000 series frame store is similar
to the 6000 series SEM. We have a 6300F equipped with such a system
made by JEOL USA. They make two systems. ARC (for image archiving)
saves the image as a TIFF file which can be saved to a floppy, optical
disk, or hard drive. The Vision system also does archiving, plus it
allows control of the microscope via an external PC.

You might first check with your JEOL representative in the Netherlands to
see if a comparable system is marketed there. If not, try contacting
Steve Hamilton at JEOL USA in Peabody, MA at 508 535-5900.

Good luck! Jim Stets
Air Products and Chemicals, Inc.
Allentown, PA
stetsjr-at-apci.com




From: Mr James Wesley-Smith :      WESLEYSM-at-biology.und.ac.za
Date: Thu, 6 Jul 1995 10:01:03 +0200 (SAST)
Subject: Polyvinyl alcohol (PVA) for cryoultramicrotomy

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Message-ID: {MAILQUEUE-101.950706100103.179-at-biology.und.ac.za}

Dear colleagues
I am having a great deal of difficulty locating water soluble PVA mol
wt 10,000 as used by Tokuyasu (1989). The suppliers approached
carry PVA only in excess of 14,000 mol wt, or products which have a
purity between 10 and 30K mol wt. Can anyone help with advise?

Thank you.


James Wesley-Smith
EM Unit
University of Natal
Durban, South Africa James Wesley-Smith
Electron Microscope Unit
George Campbell Building
University of Natal
Durban, South Africa





From: William Tivol :      tivol-at-wadsworth.org
Date: Thu, 6 Jul 1995 18:49:32 -0500 (EDT)
Subject: Re: Negative scanning & printing

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Message-Id: {9507061516.AA19918-at-pdxtw22.intel.com}

Dear John,
You wrote about finding the best equipment for scanning & printing.
I'd like to say that the best equipment can depend on what kind of nega-
tives you need to scan. I do electron crystallography, so I need accurate
quantitation of diffraction patterns. These patterns have a lot of light
area with occasional spots of high OD. In order to get what I need, the
scanner has to be one with a small light source so that the area surrounding
the dark spot doesn't transmit any light to the detection system. We have
a Perkin-Elmer 1010A, which works quite well for this, but there are other
brands with which I have had no experience. Linear diode arrays and CCD
cameras are not good for quantitating ED patterns, but they are much fas-
ter for scanning ordinary EM images.
Yours,
Bill Tivol




From: AAD123-at-aol.com
Date: Fri, 7 Jul 1995 08:19:42 -0400
Subject: subscribe

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My name is Audrey Dow and I'm a materials engineer. I would like to subscribe
to the microscopy list server. My e-mail address is AAD123-at-AOL.COM




From: Jay Jerome :      jjerome-at-isnet.is.wfu.edu
Date: Fri, 7 Jul 1995 09:56:33 -0400 (EDT)
Subject: Re: Acetone versus ethanol fixation

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We have had very good results permeabilizing cells for immunochemistry
using Brij 58 for 1-5 minutes prior to fixation and immunochemistry.
About 1% of cells are totally destroyed, about 25% don't permeabilize,
but the remaining cells label nicely with fluorescent antibodies and even
15 nm Gold conjugated antibodies. See Schliwa et al PNAS, USA 78:4329-33,
1981 for details on Brij.
Hope this helps-


Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-isnet.is.wfu.edu *
**************************************************************

On Fri, 7 Jul 1995, Felicity Lawrence wrote:

} Hi Everybody!
}
} I'm a fairly new confocalist (we have recently bought a Leica TCS 4d with
} inverted microscope). A researcher here wants to localise internalised
} structures in BHK cells infected with dengue virus. He initially fixed the
} cells with cold acetone for 1 minute but this caused dissolution, and hence
} total loss of structure, of the membrane. To minimise damage to the
} membrane, he tried fixing in ethanol. Unfortunately, the membrane remained
} intact, so much so that the antibody-FITC conjugate couldn't penetrate. He
} is now talking about slam freezing his cells, sectioning them in a
} cryoultramicrotome, followed by labelling. This seems tedious to say the least.
}
} Can someone recommend a fixation agent/schedule to give both adequate
} morphology and adequate access also?
}
} Many thanks,
}
} Felicity Lawrence
} Analytical Electron Microscopy Facility,
} QUT, Brisbane. Australia.
}




From: Stanley L Flegler :      flegler-at-pilot.msu.edu
Date: Fri, 7 Jul 1995 11:04:15 -0400 (EDT)
Subject: Optical Storage Media

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Message-Id: {9507071504.AA45095-at-pilot03.cl.msu.edu}

We've been using Panasonic 500 MB and 1 GB optical disks (actually
magneto-optical, or so I've been told) for over one year with excellent
results. We ordered our JEOL VISION package with the Panasonic Drive
installed. This is used in a multi-user lab. So far several dozen users have
purchased the 500 MB disks and have had excellent results. Nobody seems to
want 1000 images on one disk, so the 500 MB version is the most popular. They
are read-write many times. The disks have come down in price recently with the
500 MB version selling for $89.79 from Media Source, telephone 800-241-8857.
Stanley L. Flegler
Center for Electron Optics
Michigan State University
flegler-at-pilot.msu.edu




From: JOHNA-at-SCI.WFEB.EDU
Date: Fri, 07 Jul 1995 11:27:22 -0400 (EDT)
Subject: TEM studies of synthetic polymers

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Hello folks,

I need to demonstrate that routine TEM prep. methods, ie. GA fixation,
OsO4 postfix, ethanol dehydration, and spurrs embedding, can be employed to
preserve and demonstrate the presence of synthetic polymers: polymer gels,
polyacrylamide gels, polyhema, and the like.

I am wondering if any of you microscopists out in hyperspace are doing
anything like this and would share your methods, experiences, references,
and so on.

Thanks in advance for any help.

John


___________________________________________________
| |
| John G. Aghajanian, Ph.D. |
| Worcester Foundation for Experimental Biology |
| 222 Maple Avenue |
| Shrewsbury, MA 01545-2737 |
| |
| Tel: 508 842-8921 ext. 147, 161 |
| Fax: 598 842-9632 |
| JOHNA-at-sci.wfeb.edu |
| |
|_________________________________________________|





From: kennedy-at-nsi.edu (grace kennedy)
Date: Fri, 7 Jul 1995 09:25:07 -0800
Subject: NSH/LM

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X-Sender: grace-at-popmail
Message-Id: {ac231ded0002100436bc-at-[198.147.151.19]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Does anyone out there know if the National Society for Histotechnology has
a bulletin board similar to this one, and how to access it? Thanks-Reply
directly. Grace Kennedy






From: Katherine.S.Connolly-at-Dartmouth.EDU (Katherine S. Connolly)
Date: 07 Jul 95 12:56:31 EDT
Subject:

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Message-id: {17418551-at-dancer.Dartmouth.EDU}

unsubscribe
Katherine S. Connolly-at-DARTMOUTH.edu




From: wise-at-vaxa.cis.uwosh.edu
Date: Fri, 07 Jul 1995 13:30:18 +0000
Subject: Job ad

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To all:

There's an ad for a TEM technician at NIH (Bethesda, MD) on page
1963 of the latest issue of Science. Check it out if interested.

Bob


Robert R. Wise, PhD
Director, UWO Electron Microscope Facility
Department of Biology
University of Wisconsin Oshkosh
Oshkosh, WI 54901
(414) 424-3404
(414) 424-1101 fax
wise-at-vaxa.cis.uwosh.edu






From: Tina Carvalho :      tina-at-halia.pbrc.Hawaii.Edu
Date: Fri, 7 Jul 1995 10:38:23 -1000 (HST)
Subject: TEM: Freeze Sub. infiltration

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Someone just brought in a (rather sketchy) protocol for
freeze-substitution of mammalian tissue, utilizing tetrahydrofuran (THF)
or diethyl ether, followed by araldite embedment. She will be
cryofixing tissue from a marine invertebrate, probably by plunging into
liquid propane. Our main question (for now) is, Do we just start
adding Araldite to the solvent, using a "normal" schedule? Will the
Araldite mix well with either solvent? Do any of you forsee any problems?!

We are going this route to perform some EDX analysis. Any hints and tips
about any part of the proposed procedure will be greatly appreciated!


Thanks in advance!
Aloha,
Tina Weatherby Carvalho
Biological EM Facility
University of Hawaii





From: Larry Allard :      allardlfjr-at-ma160.ms.ornl.gov
Date: 8 Jul 1995 12:05:55 U
Subject: Removable storage

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Message-ID: {n1406917086.75268-at-ma160.ms.ornl.gov}

Subject: Time: 11:51 AM
OFFICE MEMO Removable storage Date: 7/8/95

A posting came through on the listserver the other day that I managed to
discard, but it involved a question of removable storage, I think using
magneto-optical technology. I thought it would be useful to alert members of
the microscopy community, esp. those overseas (or who have been hibernating
for the last several months), to the hottest new removable storage drive
system currently available, made by Iomega. The Iomega Zip drive costs $200
and uses 100 Mbyte 3.5" disks which cost $15 each in a 10-pack, or $20
singly. These drives are shipping now (but the high demand means that you
will undoubtedly find them backordered). Coming in Sept. is the Iomega Jaz
drive for $500, which will offer a 1.3 gigabyte disk, based on Winchester
technology (fast), for $100 each. For many microscopy labs, the capabilities
offered by these devices would fill a niche for image storage, transfer,
portability, convenience, etc. at very affordable prices. The Zip disks may
become an industry standard, replacing floppy diskettes, in the forseeable
future. A number of outside users of our laboratory are going to be taking
their large image files home using this technology--it seems like the way to
go for microscopists.
BTW--To qualify this post, I also own stock in the company, and if you are
smart, you'll buy some too!! (Larry's hot tip of the day!)





From: Dwight Beebe :      beebed-at-ERE.UMontreal.CA
Date: Sat, 8 Jul 1995 12:52:04 -0400 (EDT)
Subject: LM: permeabilization

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Hi,
Missed the original post, but just saw Jay Jerome's note
regarding methods of cell permeabilization. A second reference to such a
procedure for plant material is: Meiners, S. et al. Planta 1991 184:443-447.
The material used to successfully permeabilize root cells was saponin, a
plant gylcoside, available from Aldrich (cat #S130-2). Saponin, which is
rather nasty to work with, apparently is effective by itself, that is, it
is not necessary to use pectinase or cellulase in addition to the
saponin. The paper reports the incorporation of both FITC-conjugated
dextrans as well as FITC-conjugated antibodies.

Good luck!

Dwight Beebe
Institut de recherche en biologie vegetale beebed-at-ere.umontreal.ca
Dept. de sciences biologiques Voice:514-872-4563
Universite de Montreal FAX:514-872-8496
4101, rue Sherbrooke est
Montreal, PQ H1X 2B2 Canada





From: MacisNo1-at-aol.com
Date: Sat, 8 Jul 1995 17:32:55 -0400
Subject: ZIP Drives?

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I would not go investing my money in this "storage fad"! Like all the
Winchesters, Bernoulli, Syquests and Flopticals... it s magentic media and
will be obsolete in the next year. Most of the forementioned are already! The
only one with a true advantage is Syquest. For 2 reasons its reliability and
speed. (If we all agree that 14ms is fast). In any event, when rewritable
optical can obtain such competitive speeds as current Hard Drives and room
temperature blue lasers can pack over 2 Gigs on a $10 piece of media then we
have something to invest in. However ZIP's are just OK (and that is if you
can get one; they are back-ordered everwhere probably until the next
temporary "fad" hits the mail-order section of your favorite vendor). BTW- I
don't have stock in any of them nor advocate their use, but I have used a
105MG Syquest for 4 years and never had a problem with a cartridge and its
actually faster than my stock Hard Drive that came with the Macintosh. ZIP
drives are slower than Magneto Opticals... and that is really slow!
Sincerely,
Larry Kordon
Nikon, Inc.




From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Mon, 10 Jul 1995 11:53:27 GMT+1200
Subject: annoyances

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I'm struck by the number of "subscribe" and "unsubscribe" messages
which are broadcast to all subscribers.
The "subscribe" ones I can understand to a degree, people starting to
subscribe don't necessarily know much about it, but WHY, oh WHY, do
we get so many "unsubscribe" messages.

For crying out loud:

TO UNSUBSCRIBE, send an email message to:

LISTSERVER-at-AAEM.AMC.ANL.GOV

message should be:

UNSUBSCRIBE MICROSCOPY YOURID-at-YOURADDRESS

DON'T SEND IT TO MICROSCOPY-at-AAEM.AMC.ANL.GOV, OR IT GETS BROADCAST
TO EVERYONE ON THE MAILING LIST

It's pretty simple isn't it?

Ritchie Sims
Geology Department
University of Auckland
Auckland, New Zealand




From: jfmjfm-at-umich.edu (John F. Mansfield)
Date: Mon, 10 Jul 1995 09:53:26 -0400
Subject: annoyances

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X-Sender: jfmjfm-at-srvr5.engin.umich.edu
Message-Id: {v02120d0cac26e122ee9b-at-[141.213.21.13]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Check out our new Home Page at Michigan with link to much microscopy info.
URL=http://www.engin.umich.edu/microscopy/
OK?

Jfm

John Mansfield
North Campus Electron Microbeam Analysis Laboratory
413 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (313)936-3352 FAX (313)936-3352
jfmjfm-at-engin.umich.edu, John.F.Mansfield-at-umich.edu
or jfmjfm-at-umich.edu they all reach me!
URL: http://www.engin.umich.edu/~jfmjfm/jfmjfm.html






From: jfmjfm-at-umich.edu (John F. Mansfield)
Date: Mon, 10 Jul 1995 12:21:10 -0400
Subject: Re:

Contents Retrieved from Microscopy Listserver Archives
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} } Check out our new Home Page at Michigan with link to much microscopy info.
} } URL=http://www.engin.umich.edu/microscopy/
} } OK?
} }
} } Jfm
} }
} }
} I get a "not found" message if I try to use the URL:
}
} http://www.engin.umich.edu/microscopy/
}
Whoops it should have been:
} URL=http://www.engin.umich.edu/microscopy
I'll try and fix it so both versions work. Until then the URL should be:
} URL=http://www.engin.umich.edu/microscopy

John Mansfield
North Campus Electron Microbeam Analysis Laboratory
413 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (313)936-3352 FAX (313)936-3352
jfmjfm-at-engin.umich.edu, John.F.Mansfield-at-umich.edu
or jfmjfm-at-umich.edu they all reach me!
URL: http://www.engin.umich.edu/~jfmjfm/jfmjfm.html






From: Ian Hall :      hall-at-me.udel.edu
Date: Mon, 10 Jul 1995 12:25:13 -0400 (EDT)
Subject: Postdoc Position

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POSTDOCTORAL POSITION IN ELECTRON MICROSCOPY

A postdoctoral position is available immediately involving research
in the microstructural characterization of hard magnetic materials,
magnetic thin films, and nanoparticles. The research involves extensive
Transmission Electron Microscopy studies on a wide variety of materials
using JEOL JEM 2000 FX and JEOL JEM 100C TEM's, and an AMRAY 20 SEM.
Candidates must have experience in conventional and high resolution
TEM, Lorentz microscopy, energy dispersive X-ray analysis, and X-ray
diffraction. Appointment is for one year. Send a resume and name,
address, phone, and FAX of 3 references by August 1, 1995 to:
Professor George C. Hadjipanayis, Dept. of Physics and Astronomy,
University of Delaware, Newark, DE 19716-2570, USA, FAX 302-831-1637.

The UNIVERSITY OF DELAWARE is an Equal Opportunity Employer which
encourages applications from qualified minority groups and women.










From: bafpjec-at-uxa.ecn.bgu.edu (Joyce Craig)
Date: Mon, 10 Jul 1995 14:21:41 -0500
Subject: subscribe

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Pleaseput me on the microscopy listserver again.
Joyce Craig
Biology Department
Chicago State University
9501 King Drive
Chicago, IL 60628


Joyce Craig
Biology Department
Chicago State University
9501 King Drive
Chicago, IL 60628





From: hmeekes-at-biosci.mbp.missouri.edu (Herman Meekes)
Date: Mon, 10 Jul 1995 16:55:14 -0500
Subject: electron diffraction

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Dear list readers!

Although I have many years of experience in TEM, I have never done any
electron diffraction studies. Could anyone who has experience in that field
point me to a comprehensive text on electron diffraction: how and why it
works, procedures, interpretation of patterns etc.? It looks like I will be
going to try some steps in that direction.

Thanks for your time and help!





Herman Meekes
Biological Sciences ______________ ______________
University of Missouri ---__ \ / __---
109 Tucker Hall ------__\---/__------
Columbia, MO. 65211, USA \( )/
Tel: 314-882-0171 V
Fax: 314-882-0123 / \
e-mail: hmeekes-at-biosci.mbp.missouri.edu /___\






From: William Tivol :      tivol-at-wadsworth.org
Date: Mon, 10 Jul 1995 19:22:47 -0500 (EDT)
Subject: Re: electron diffraction

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}
} Dear list readers!
}
} Although I have many years of experience in TEM, I have never done any
} electron diffraction studies. Could anyone who has experience in that field
} point me to a comprehensive text on electron diffraction: how and why it
} works, procedures, interpretation of patterns etc.? It looks like I will be
} going to try some steps in that direction.
}
} Thanks for your time and help!
}
Dear Herman,

This list is not complete, but it will hold you for a while:

Diffraction Physics by Cowley
Electron Diffraction Techniques by Cowley
Electron Diffraction by Pinsker
Structure Analysis by Electron Diffraction by Vainshtein
Electron Microscopy of Thin Crystals by Hirsch, Howie, Nicolson, Pashley & Whelan

Good luck.
Yours,
Bill Tivol




From: Dr. jiechao Jiang :      jiangj-at-papin.hrz.uni-marburg.de
Date: Tue, 11 Jul 1995 12:21:35 +0000
Subject: subscribe

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Message-Id: {9507111022.AA11743-at-HRZ.Uni-Marburg.DE}
Sender: {jiangj-at-papin.hrz.uni-marburg.de}

____________________
Dr. Jiechao Jiang
Philipps-Universitaet Marburg
Institut fuer Geologie & Palaeontologie
Hans-Meerwein-Str.
35043 Marburg

Tel. +49 6421 28-3458
Fax. +49 6421 28-8919
E-Mail: Jiangj-at-Mailer.Uni-Marburg.De




From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 11 Jul 1995 12:31:31 -0400
Subject: RE-BooksOnElectDiffr

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Message-ID: {n1406656373.26071-at-mse.engin.umich.edu}
"RE:Books/ED" {microscopy-at-aaem.amc.anl.gov}
X-Mailer: Mail*Link SMTP/QM 3.0.0GM

Subject: Time: 12:08 PM
OFFICE MEMO RE:BooksOnElectDiffr Date: 7/11/95

For soimeone just starting out in electron diffraction, I would recommend
first trying one or all of the following:
Chapter 15, "Electron Diffraction" by Alderson & Halliday in the book
TECHNIQUES FOR ELECTRON MICROSCOPY, Ed: D. H. Kay, F. A. Davis
Publishers, 2nd. Ed. 1965
Chapter 2, "Electron Diffra tion in the Electron Microscope" in the
book PRACTICAL ELECTRON MICROSCOPY IN MATERIALS SCIENCE,
by J. W. Edington. Originally published by Van Nostrand, 1976
and now out of print, but possibly available from
TechBooks, 301-871-1663.
Both of the above give a gentle introduction to ED, with good illustrations
and worked examples, etc. You may also find the following helpful, after
you've read the above:
INTERPRETATION OF ELECTRON DIFFRACTION PATTERNS, by Andrews,
Dyson & Kewon, Plenum Press, i967
All of these are rather 'old' books and may be hard to find. You probably
can't buy any of them, but may find them in a large scientific library. I
don't know of any recent book that treats the subject in an easy way that
would be useful to a beginner. If you have questions, don't hesitate to
contact me (bigelow-at-umich.edu), W. C. Bigelow





From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 11 Jul 1995 12:41:15 -0400
Subject: RE-ElecDiffBooks

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Message-ID: {n1406655768.62723-at-mse.engin.umich.edu}

Subject: Time: 12:33 PM
OFFICE MEMO RE:ElecDiffBooks Date: 7/11/95

For someone just starting out in electron diffraction, I would recommend
first trying the following:
Chapter 15, "Electron Diffraction" by Alderson & Halliday in the book
TECHNIQUES FOR ELECTRON MICROSCOPY, Ed: D. H. Kay, F. A. Davis
Publishers, 2nd. Ed. 1965
Chapter 2, "Electron Diffraction in the Electron Microscope" in the
book PRACTICAL ELECTRON MICROSCOPY IN MATERIALS SCIENCE,
by J. W. Edington. Originally published by Van Nostrand, 1976
and now out of print, but possibly available from
TechBooks, 301-871-1663.
Both of the above give a gentle introduction to ED, with good illustrations
and worked examples, etc. You may also find the following helpful, after
you've read the above:
INTERPRETATION OF ELECTRON DIFFRACTION PATTERNS, by Andrews,
Dyson & Kewon, Plenum Press, i967
All of these are rather 'old' books and may be hard to find. You probably
can't buy any of them, but may find them in a large scientific library. I
don't know of any recent book that treats the subject in an easy way that
would be useful to a beginner. If you have questions, don't hesitate to
contact me (bigelow-at-umich.edu), W. C. Bigelow





From: John W Heckman :      heckman-at-pilot.msu.edu
Date: Tue, 11 Jul 1995 14:27:45 -0400 (EDT)
Subject: Re:diffraction,e-,books.

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Dear Herman Meekes,

I had some years of TEM experience in (mostly) biological studies, and was
similarly confronted by the need to learn something of electron diffraction
techniques. I concur with W.C. BigelowUs opinion about the Edington text.
Last I heard, it was about $32.00, paper bound still from Van Nostrand
Reinhold, NY (ISBN 0-961-2934-0-3). Another gentle introduction is in the
Glauert series Vol 1 part II, An Introduction to Electron Diffraction by
B.E.P.Beeston. Still another one that may help is G. Thomas and M.
Goringe,
Transmission Electron Microscopy of Materials , Wiley-Interscience. 1979.
ISBN 0-471-12244-0. Hope this helps.

Cheers,
John Heckman
heckman-at-pilot.msu.edu





From: David Pejchl :      david-at-ISIBrno.Cz
Date: Tue, 11 Jul 1995 07:07:13 +0200
Subject: Re: ZIP Drives?

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} To: Microscopy-at-aaem.amc.anl.gov
} Subject: ZIP Drives?
}
} I would not go investing my money in this "storage fad"! Like all the
} Winchesters, Bernoulli, Syquests and Flopticals... it s magentic media and
} will be obsolete in the next year. Most of the forementioned are already! The
} only one with a true advantage is Syquest. For 2 reasons its reliability and
} speed. (If we all agree that 14ms is fast).

Yes, that's true, BUT ...
As I heard, there are problems with reliability of the data storage.
Problems were found on 270MB mechanics (SCSI or AT), but not on 105MB.
The main problem
is dust.If any dust penetrates into disk , the disk media can be (will be)
scratched. In Bernoulli system, there is no such high danger. But the main
problem here is price for the mechanics as well as for the data media.

Does anybody know something more about IOMEGA Jaz drive???? The relation
between capacity and the price seems to be fine!

} 105MG Syquest for 4 years and never had a problem with a cartridge and its

David Pejchl



========================================================================
Institute of Scientific Instruments | E-mail: david-at-ISIBrno.Cz
Academy of Sciences of the Czech Republic | pejchl-at-Sci.MUni.Cz
Kralovopolska 147, 612 64 Brno | Phone: +42 5 41321246
Czechland | Fax: +42 5 41211168
========================================================================








From: s.griffiths-at-ucl.ac.uk (Stephen Griffiths)
Date: Wed, 12 Jul 1995 12:57:41 +0100
Subject: TEM: Flat embedding Vibratome Sections: Problems.

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I am trying to find a reliable way of getting 50 micrometre Vibratome
sections of paraformaldehyde/glutaraldehyde perfusion fixed brain tissue to
embed absolutely flat. I need to resection them to 1 micrometre and then
section the semithins to ultrathins.
The tissue is HRP/DAB labelled and I am trying to find and section
individual cells to carry out image analysis.

The vibratome sections are fairly large in area (up to 20 x 10mm) and there
are a lot of them (50 to 100).

At the moment I fix them in Osmium Tetroxide as flat as possible on a glass
surface, but they always buckle slightly. After dehydration and infiltration
each section is mounted in resin on a PTFE coated slide under a silicon
coated coverslip.

This is where the first problem arises. If the coverslip is pressed hard
enough to flatten the section, it breaks up due to the stiffening effect of
the Osmium and the buckled nature of the section.

The smaller sections that do survive are frequently still too buckled and
uneven to use the 50 or 100 times objective mag on the LM. This makes
focusing and photography very difficult.

I have tried Osmium fixing the sections between a slide and coverslip, but
if they are pressed hard enough to keep them flat, the fix does not
penetrate to the centre of the section.

Although I achieve some results with my 'buckled' sections, the whole
process would be more reliable and reproducible if the large 50=B5m sections
were flat in the first place.

The only methods I have seen all seem to be on much smaller sections, but I
do need to have a lot of large sections.=20

I'm sure someone else must be doing this sort of thing and that there is a
relatively simple way of achieving this goal.

Does anybody have a method or a reference to a well described method?=20

All and any suggestions gratefully received.
TIA.

Regards

Stephen Griffiths.

{} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {}
{} Stephen Griffiths {} e-mail s.griffiths-at-ucl.ac.uk {}
{} Visual Science Department {} {}
{} Institute of Ophthalmology {} Tel: 0171 608 6914 {}
{} London. EC1V 9EL. UK. {} Fax: 0171 608 6850 {}
{} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {}





From: jeanne_barker-at-merck.com (Jeanne Barker)
Date: 12 Jul 1995 08:28:43 U
Subject: Cryosectioning of unfixed c

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REGARDING Cryosectioning of unfixed cells
Good morning,

A colleague of mine is trying to cut 5 micron sections of unfixed Cos cells.
This is the first time that she is working with unfixed cells, and she is
having a little problem. The cells were embedded in agar, then frozen in OCT.
The cells look terrible. There is not enough time, or enough of the cells to
play around with the different variables, so I am asking if anyone has any
suggestions that I can pass on to her, or any references that discuss
protocols that might help her.

Thank-you very much,
Jeanne






From: William Tivol :      tivol-at-wadsworth.org
Date: Wed, 12 Jul 1995 16:07:00 -0500 (EDT)
Subject: Re: Cryosectioning of unfixed c

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} [snip] The cells were embedded in agar, then frozen in OCT.
} The cells look terrible. There is not enough time, or enough of the cells to
} play around with the different variables, so I am asking if anyone has any
} suggestions that I can pass on to her, or any references that discuss
} protocols that might help her.
}
Dear Jeanne,
My guess is that the freezing is not fast enough to prevent ice crystal
formation. High-pressure freezing might be good enough to preserve several-
micron-thick specimens, but neither plunge-freezing nor slam freezing will
preserve whole cells, especially cells embedded in agar. If your colleague
does not have access to a high-pressure freezer, perhaps a call to Balzers
will help. Good luck.
Yours,
Bill Tivol




From: Mehmet Sarikaya :      sarikaya-at-u.washington.edu
Date: Wed, 12 Jul 1995 15:25:29 -0700 (PDT)
Subject: EM Position Open

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---------- Forwarded message ----------




Electron Microscopist Position Open

Applications are invited for an Electron Microscopist in the Department
of Materials Science and Engineering, at the University of Washington,
specializing in maintaining and training of scanning and transmission
electron microscopes and use of related techniques in materials and/or
biological sciences at the Electron Microscopy Consortium. The
microscopist is expected to (i) perform day-to-day alignment, operation,
service, and maintanence of the instruments, (ii) train basic uses of the
instrument, (iii) and prepare SEM and TEM samples and (iv) perform
analysis of material in conjunction with researchers using these
instruments. A strong background in the operation of SEMs and TEMs is
essential and additional experience with STEM, EDS, EELS techniques and
microscopy software is desirable. Duties also include supervision of
thin film and bulk sample preparation by ultramicrotoming and ion-beam
milling of inorganic and biological samples. Materials projects involve,
small particles, metals, ceramics, composites, including polymers and
biological hard tissues, geological and beam-sensitive materials. A
strong background in a field of materials science, biology, chemistry,
solid-state physics, or geology is also important.

Applicant should send a complete package, including (i) a letter of
interest with a statement of research and career objectives, (ii) a
resume with publications and a list of instrumental expertise, (iii)
names of three references, by August 31, 1995 to:

Prof. Mehmet Sarikaya
Materials Science and Engineering
Box 352120
University of Washington
Seattle, WA 981920-2120

Fax (206) 543-6381
eMail Sarikay-at-U.Washington.edu








From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Wed, 12 Jul 1995 17:26:47 -0700
Subject: Freehand vs Illustrator?

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Greetings:

We have recently gone digital and now everyone is asking what is the best
way to compose and label pictures etc. We use Macs and Photoshop to get our
images, now the question is what is the best way to add labels etc. and
prepare them for printing.

I know this will generate a lot of personal preferences. That's OK, I have
an open mind and just want to know whats going on in this area. Freehand,
Illustrator, Quark, Pagemaker, all will do the job, but we have learned
that sometimes certain applications are better for specific jobs. I would
like to take advantage of your collective experiences to learn more and
help the users in my lab.

Jonathan Krupp
Microscopy and Imaging Lab
University of California
Santa Cruz, CA 95064
(408) 459-2477
jmkrupp-at-cats.ucsc.edu






From: Mev H van der Merwe :      HVDM-at-op1.up.ac.za
Date: Thu, 13 Jul 1995 15:08:06 GMT+2
Subject: Technical Anatomy

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Can anyone please give me information and mixing instructions on:
Biodur E20 Red
Biodur E20 (hardner)
Biodur Thinners E
For use of making a cast of fine bloodvessels, or any other
suggestions?

thanks

H. van der Merwe
University of Pretoria
Faculty of Veterinary Science
Department of Anatomy
Onderstepoort
0 1 1 0
SOUTH AFRICA

E-mail:HvdM-at-opl.up.ac.za




From: Paul Webster :      Paul.Webster-at-QuickMail.Yale.edu
Date: 13 Jul 1995 09:46:44 -0400
Subject: Re: cryo on unfixed cells

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Message-Id: {n1406493610.56458-at-QuickMail.Yale.edu}

Jeanne Barker asks about sectioning frozen, unfixed cells;
"The cells were embedded in agar, then frozen in OCT.
The cells look terrible. There is not enough time, or enough of the cells to
play around with the different variables, so I am asking if anyone has any
suggestions that I can pass on to her, or any references that discuss
protocols that might help her."
Bill Tivol is correct in pointing ou the concequences of freezing damage, a
variable that is usually ignored by histologists cutting sections for light
microscopy. However, I am not sure that the researchers involved in this
project would have the time, skill or patience to start preparing their cells by
high pressure freezing (BTW Muller lab URL is
http://www.em.biol.ethz.ch/em-lab/emhome.html).

It would be much simpler to fix the cells and then cryoprotect them before
freezing. Liquid nitrogen can then be used for freezing without having to worry
about vitrification. Simply immersing the fixed cells into a sucrose solution
(our histologists use 10%) is usually sufficient for cryostat sectioning.

For really thin sections and improved resolution we routinely prepare sections
(300 - 500 nm thick) of formaldehyde fixed material that has been cryoprotected
in 2.1 M sucrose (the Tokuyasu method). The sections are cut at -60*C in a
cryochamber of an ultramicrotome with a dry knife and then transferred onto
glass slides, but this might be too involved for routine work.

Be aware that the sucrose will make the block softer so lower cutting
temperatures may have to be used.

Paul Webster
Center for Cell Imaging
Yale School of Medicine





From: wise-at-vaxa.cis.uwosh.edu
Date: Thu, 13 Jul 1995 09:03:47 +0000
Subject: Sapphire knives

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Thanks to all who responded to my request regarding information on
sapphire knives. I thought I would provide a synopsis of what I have found
out. My original question was one of mostly curiosity. Perhaps others are
curious as well.

At least two Japanese companies (one named Sakura, the other ?)
have been making sapphire knives for some time. They are both still in
business although they do not seem to currently have a distributor in the
US. The last catalog to list sapphire ultramicrotome knives for sale in
the US was the 1988 BioRad Microscopy Accessories catalog (6th edition).
Their "Crystome" sapphire knife for ultramicrotomy listed for $1495 (5mm
cutting edge, synthetic sapphire).

The BioRad inventory has been sold several times in the last 3
years and most of it resides with Structure Probe Inc and Energy Beam
Sciences. Both EBS and SPI continue to support the BioRad catalog such
that an order placed with BioRad is forwarded to and processed by SPI or
EBS (depending on the product). Steve Slap of Energy Beam Sciences says
that although EBS does not maintain a stock of sapphire knives, a quick fax
by EBS to the suppliers in Japan is all that is needed to order one. Also,
SPI has several sapphire knives in stock in the US, according to Chuck
Garber. EBS markets a sapphire Vibratome knife, but it is not designed for
ultramicrotomy.

No one who responded to my original query had actually used a
sapphire knife for ultramicrotomy. One person knew someone who had
conducted tests with a sapphire ultramicrotome knife on a tough sample
years ago and found that it dulled significantly faster than diamond. This
latter person thought that sapphire might be adequate for ultrathin
sectioninng of soft (biological) tissues but not harder samples.

The distinct advantage of sapphire over diamond is that knife
widths of } 5mm are quite possible (because the material is synthetically
manufactured), making them attractive to histologists. For instance, the
sapphire Vibratome knife from EBS is } 25 mm wide and is far superior in
sectioning performance to glass histology knives. Diamond knives in that
size range are, quite obviously, unavailable (unless you want to buy the
Hope Diamond and have it made into a histology knife--NOT).

Apparently a US market for sapphire ultramicrotome knives never
developed. Probably (according to Chuck Garber) because domestic (US)
suppliers of diamond knives solved some early problems with quality and
availability and also because the Yen became so strong against the dollar
that the price difference between sapphire (produced in Japan) and diamond
(produced in the US) shrank. I do not know what the situation is in other
parts of the world. Are there any microscopists in Japan who are on the
net and could provide some information? Because they are still available
in Japan, I am guessing there must be a market for them there (?).

Bob

Robert R. Wise, PhD
Director, UWO Electron Microscope Facility
Department of Biology
University of Wisconsin Oshkosh
Oshkosh, WI 54901
(414) 424-3404
(414) 424-1101 fax
wise-at-vaxa.cis.uwosh.edu






From: Dennis C. Winkler :      dennis.winkler-at-NIST.GOV
Date: Thu, 13 Jul 1995 09:55:44 -0400 (EDT)
Subject: Re: JEOL 2010 to Mac Interface

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Microscopy Listserver Posting {microscopy-at-aaem.amc.anl.gov}
Message-id: {dennis.winkler.1156031384A-at-ENH.NIST.GOV}
X-Mailer: VersaTerm Link v1.1.6
Content-transfer-encoding: 7BIT

In response to Colin Veitch's post re: downloading/uploading
TEM settings to a PC:

} Is anyone out there using a Mac for a similar purpose and if so could they
} give us some advice on the code required. We are reluctant to use a PC as
} that would mean bringing in yet another computer into the TEM room as there
} are already 3 in there.

...I can offer this:

We are currently using a Mac (Quadra) running 'SoftPC with Windows' in place
of an IBM PC for saving alignments on a Philips CM30. Communication is over
an RS232 line. The situation sounds similar to yours. Since we already had
the IBM software and the Mac software was not yet available, it was a quick
fix that we are still using (about a year later). One thing to note is that
the IBM software runs slower in emulation on a Mac, but saving and loading
settings is still done rather quickly (several seconds). We plan to
eventually switch to the Mac-based remote control software.

-Dennis
____________________________________________________
Dennis C. Winkler
National Institute of Standards and Technology (NIST)
CSTL - Surface and Microanalysis Science Division
Microanalysis Research Group
(301) 975-2184
dcw-at-enh.nist.gov
(Disclaimer: These are my opinions and not the official position of NIST.)




From: PRING :      richard.pring-at-bbsrc.ac.uk
Date: Thu, 13 Jul 1995 11:40:54 -0500
Subject: 1) eds LINK/PC INTERFACE 2)Crystal on SEM

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X400-Received: by mta bottom.ctd.anl.gov in /PRMD=ANL/ADMD= /C=US/; Relayed;
Thu, 13 Jul 1995 10:41:46 -0500
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Thu, 13 Jul 1995 10:41:45 -0500
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Does anyone have any ideas on interfacing a Link AN 10000 with imaging
programmes to a PC? I gather that Link once made a device but since producing
PC based systems, this is no longer available. Does anyone else produce a
nsimilar item? Please keep any replies SIMPLE - I am a fully paid up member of
"computer illiterates"
Secondly, anyone have any experience of the "Crystal" imaging system once
available as an accessory for SEM's?

Many Thanks

Richard

Richard Pring
Long Ashton Research Station
Long Ashton
Bristol, UK. BS18 9AF

richard.pring-at-bbsrc.ac.uk





From: Doug Davis :      doug_davis-at-maillink.berkeley.edu
Date: 13 Jul 1995 09:10:42 -0700
Subject: subscribe

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Message-ID: {n1406495960.18573-at-maillink.berkeley.edu}

Subject: Time: 9:09 AM
OFFICE MEMO subscribe Date: 7/13/95

Please subscribe





From: Glen Macdonald :      glenmac-at-u.washington.edu
Date: Thu, 13 Jul 1995 11:41:32 -0700 (PDT)
Subject: Re: Sapphire vibratome knives

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X-Sender: glenmac-at-homer01.u.washington.edu

A friend tried out a sapphire ultra knife when they first appeared.
Epon embedded tissue dulled it quite quickly. And lower cost, high
quality diamond knives appeared and became the obvious choice to purchase

Several years ago a sales rep. from one of Leica's predecessor
companies (can't remember exactly what the company was called then)
loaned us a sapphire vibratome blade to try out. It was about 4 cm long
and 1 cm wide, shaped like a single edge injector blade, but transparent
crystal. There wasn't much improvement over disposable razor blades for
fixed tissue, but it was wonderful for soft things like unfixed embryos
sectioned for organ culture.

Glen MacDonald
Hearing Development Laboratories
University of Washington
Seattle, WA 98195-6515
(206)543-8360
glenmac-at-u.washington.edu






From: spignole-at-ix.netcom.com (Susanne Brandom)
Date: Thu, 13 Jul 1995 14:11:24 -0700
Subject: Need pictures taken of microbes

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I have a client that would like to have pictures taken of 40 to 50
micron microbes used to degrade hydrocarbons in soil (patented
process). He is interested in both optical and EM. If you can
provided this service, please send me following information. PLEASE
check your send address. It should be spignole-at-ix.netcom.com or
spb-at-wwa.com.

OPTICAL:

Type of optical microscope:

What techniques would you use ie phase contrast or Hoffman modulated
contrast? Other?

Under what conditions can you grow the cultures?

Methods of fixation available:

Type of output: polaroid 35 mm digital format

Charges:

SEM

Type of instrument:

How would you prepare the sample?

Variable pressure or E-SEM

CryoSEM

Type of output polaroid or digital
Charges:

Thanks:

Susanne Pignolet Brandom Ph.D.
MicroWorld Resources and News
708-548-6522






From: STEVEN E SLAP :      75767.640-at-compuserve.com
Date: 14 Jul 95 07:34:21 EDT
Subject: Re: Sapphire vibratome knives

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We developed the sapphire blade for the Vibratome at the end of 1990 as a joint
project with a company with ultramicrotomy knife experience and with the
assistance of Dr. Madhu Kalia, Dept. of Pharmacology, Thomas Jefferson
University. Technical Products International, manufacturer of the Vibratome,
offers sapphire knives for the Vibratome through its worldwide distributors
(there are 5 Vibratome distributors in the USA, including my company, Energy
Beam Sciences). You can contact Ralph Willen at TPI toll-free at 800-729-4421
for more information. Using these sapphire blades, our customers have done
routine serial sectioning of mildly fixed tissue with a Vibratome 1000 of
thicknesses from 5-10 microns, revealing subcellular features never before seen
in Vibratome sections (with microscopic resolution similar to that obtained with
1 micron plastic sections). With a Vibratome 2000, sections as thin as 2
microns have been obtained.
Note:sapphire knives can be resharpened indefinitely.
Steven Slap, Energy Beam Sciences





From: waheeschen-at-dow.com (Bill Heeschen (517)636-4005 PMRC/ASL)
Date: Fri, 14 Jul 1995 12:46:41 -0400
Subject: IA: Request info about Pias Co. image analyzers, PIAS-2

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Message-Id: {199507141646.AA01011-at-na3.dow.com}


This request is being posted on the "microscopy" and "nih-image" listservers

Folks:

If anyone has experience with image analyzers from the Pias Co., particularly
the model PIAS-2, please contact me privately (e-mail, phone, whatever).

Thanks in advance.

Bill
==========================
Bill Heeschen/Analyt. Sci.- Mat. Char. -----
1897-F Building / The Dow Chemical Co. --- ---
Midland, MI 48667 U.S.A. --- D O W ---
phone: (517)636-4005 fax: (517)636-5453 --- ---
Email: waheeschen-at-dow.com -----
========================== R




From: minter-at-Kodak.COM (John Minter)
Date: Fri, 14 Jul 1995 13:06:46 -0400
Subject: Philps CM to Mac Interface

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Dennis Winkler (dcw-at-enh.nist.gov) wrote:

} ...I can offer this:
}
} We are currently using a Mac (Quadra) running 'SoftPC with Windows' in place
} of an IBM PC for saving alignments on a Philips CM30. Communication is over
} an RS232 line. The situation sounds similar to yours. Since we already had
} the IBM software and the Mac software was not yet available, it was a quick
} fix that we are still using (about a year later). One thing to note is that
} the IBM software runs slower in emulation on a Mac, but saving and loading
} settings is still done rather quickly (several seconds). We plan to
} eventually switch to the Mac-based remote control software.

Please explain what you mean by "the Mac-based remote control software."
Does such a product currently exist????


Best Regards,
John

John R. Minter, Ph. D. Phone: (716) 722-3407
Eastman Kodak Company FAX: (716) 477-3029
Analytical Technology Division email: minter-at- kodak.com
Rochester, NY 14562-3712






From: Robert Fisher :      rmfisher-at-u.washington.edu
Date: Fri, 14 Jul 1995 10:05:59 -0700 (PDT)
Subject: UW EM POSITION - Clarification (fwd

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The opening for an electron microscopist in the Materials
Science department at the Univ. of Washington, announced here
on July 12, is a staff position NOT a faculty position.
A Ph.D. is not required.

The job includes some opportunities for collaborative research
on funded projects.

Mehmet Sarikaya

Sarikaya-at-U.Washington.Edu





From: Dennis C. Winkler :      dennis.winkler-at-NIST.GOV
Date: Fri, 14 Jul 1995 16:12:50 -0400 (EDT)
Subject: Re: Philps CM to Mac Interface

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} Please explain what you mean by "the Mac-based remote control software."
} Does such a product currently exist????

} Best Regards,
} John R. Minter, Ph. D.

When I looked into it a year ago, Philips gave me a part number (PW6444/00).
We will soon be getting a Philips CM300 and, as we understand it, we will be
getting the Mac-based remote control software.

Hope this helps,
Dennis
____________________________________________________
Dennis C. Winkler
Surface and Microanalysis Science Division
National Institute of Standards and Technology (NIST)
(301) 975-2184 dcw-at-enh.nist.gov
(Disclaimer: These are my opinions and not the official position of NIST.)




From: dlb-at-aruba.ccit.arizona.edu (David Bentley)
Date: Fri, 14 Jul 1995 13:53:53 -0700
Subject: Polyvinyl alcohol for cryoultramicrotomy

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We also had difficulty getting low molecular weight Polyvinyl
alcohol (PVA). The suppliers tech people offered differing testing
procedures changed the molecular weights seen, dramatically. Some calling
around, showed the concern, among people doing cryoultramicrotomy, was that
the viscosity remained low, with good soluability in water, rather than a
strict question of molecular weight. 30,000-70,000, molecular weight, (4-6
cps) PVA was purchased from Sigma, (Catalog # P-8136) that is "cold water
soluable" This has worked well in our lab for cryoultramicrotomy. I'd be
interested in what others success has been with various PVA's.
later
dlb





From: bozzola-at-siu.edu (John. J. Bozzola)
Date: Fri, 14 Jul 1995 16:56:05 -0600
Subject: Microscopy File Servers

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We are interested in setting up some sort of file server for image
retrieval purposes. Images would be put into the server from a variety of
microscopes (light, SEM, TEM). Our current LAN consists of several PC's,
Macs and a SUN Sparc 5 workstation that is used on our Voyager III EDX
system. What would be needed to set up a fast, large capacity server?
Suggestions or names of others to contact would be appreciated. Thank you.

#############################################################################
John J. Bozzola, Director
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
#############################################################################






From: FRANCISCO J HERNANDEZ BLAZQUEZ fzea - zab 0195 616122 - 283 :      fjhblazq-at-usp.br
Date: Fri, 14 Jul 1995 21:26:42 -0500 (CDT)
Subject: Re: cryo on unfixed cells

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An alternative method to improve the morphology is to immerse the tissue in
freon in
a container immersed in liquid nitrogen. The freezing is faster because
the freon doesn't form the thin isolating layer of gas around the
specimen as occurs when the tissue is frozen in liquid nitrogen.
=============================================================================
Francisco Javier Hernandez Blazquez |
Universidade de Sao Paulo - Faculdade de | e-mail fjhblazq-at-usp.br
Zootecnia e Engenharia de Alimentos | Voice: 55 195 616122 r. 265
Departamento de Ciencias Basicas/Histologia| r. 278
Av. Duque de Caxias Norte, 225 CP 23 | Fax: 55 195 611689
CEP 13630-000 Pirassununga (Sao Paulo) | 55 11 8694831
BRAZIL |
==============================================================================







From: Willem.Jacob :      jacob-at-sch2.uia.ac.be
Date: Sat, 15 Jul 1995 14:05:16 +0200 (MET DST)
Subject: subscribe tome

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please subscribe Fernando tome from INSERM U153 in Paris please
email address: fmstome-at-citi2.fr
Thanks Annette




From: Dr. Andrew P. Somlyo :      aps2n-at-elvis.med.virginia.edu
Date: Sat, 15 Jul 1995 10:51:19 -0400 (EDT)
Subject: Re: cryo on unfixed cells

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NONSENSE

On Sat, 15 Jul 1995, Reinhard Rachel t4534 wrote:

} I would not believe that there are still some people on this
} earth using Freon or similar compounds, if I had not read
} this e-mail. Freon should not be used any more, at all.
} Liquid Propane or Ethane do the same job.
} BUT: even immersing in Freon, Propan, or Ethan is not good enough
} to freeze a sample with a thickness of more than a few microns without
} damaging it. Only high pressure freezing will do it (up to a thickness
} of 200 to 500 micron; see papers by M.Mueller et al. Zuerich).
} If you can't do this, you will have to fix your tissue chemically in
} advance and possibly cryo-protect it before freezing. This MAY
} POSSIBLY give you a proper ultrastructure, depending on your
} tissue or cells, if you are lucky.
} Without this, your sample will be damaged during freezing and
} substitution. --- Best regards,
}
} - - Reinhard Rachel {Reinhard.Rachel-at-biologie.uni-regensburg.de}
} | )| ) Universitaet Regensburg, Lehrstuhl fuer Mikrobiologie
} | \| \ D - 93040 Regensburg (Tel.: xx49-941-943-4534)
}
}




From: gbza40-at-udcf.gla.ac.uk
Date: Sun, 16 Jul 1995 12:22:50 +0100
Subject: re-subscribe after vacation

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subscribe microscopy gbza40-at-udcf.gla.ac.uk





From: Gerd.Knoll-at-uni-konstanz.de (Gerd Knoll)
Date: Sun, 16 Jul 1995 14:05:23 +0000
Subject: Address of Eico Engineering?

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To: Microscopy-at-aaem.amc.anl.gov

Dear list-participants,

does anyone know how to contact Eico Engineering (Japan) the easiest way
from Germany; is there a German or European dealer or address?

I am interested in their MF-2 quick freezing device equipped with a
magnetron; does anyone have any experiences and information about performance?

many thanks
Gerd

--------------------------------------------
Gerd Knoll (gerd.knoll-at-uni-konstanz.de)
Fac Biol, Univ Konstanz, Postfach 5560
D-78434 Konstanz, Germany
--------------------------------------------





From: zhenquan liu :      zqliu-at-pku.edu.cn
Date: Sun, 16 Jul 1995 19:52:35 -0600 (CST)
Subject: EDAX DX-4

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X-Nupop-Charset: English

I would like to know the email address of EDAX EDS service in USA,
so that I can contact them. I have a DX-4 EDS here, and have some
problems. If I cannot get the address, then I will show these
problems to the public for help.

Therefore, if any one who knows the address, please tell me.

Many thanks.

Zhen Quan Liu

EM laboratory
Peking University
Beijing 100871
China

FAX ( 86 10 ) 256 1615

Email zqliu-at-pku.edu.cn




From: zhenquan liu :      zqliu-at-pku.edu.cn
Date: Sun, 16 Jul 1995 20:04:39 -0600 (CST)
Subject: EDAX DX-4

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X-Nupop-Charset: English



I would like to know the email address of EDAX EDS service in USA,
so that I can contact them. I have a DX-4 EDS here, and have some
problems. If I cannot get the address, then I will show these
problems to the public for help.

Therefore, if any one knows the address, please tell me.

Many thanks.

Zhen Quan Liu

EM laboratory
Peking University
Beijing 100871
China

FAX ( 86 10 ) 256 1615

Email zqliu-at-pku.edu.cn




From: zhenquan liu :      zqliu-at-pku.edu.cn
Date: Sun, 16 Jul 1995 20:18:02 -0600 (CST)
Subject: EDAX DX-4

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X-Nupop-Charset: English


I would like to know the email address of EDAX EDS service in USA,
so that I can contact them. I have a DX-4 EDS here, and have some
problems. If I cannot get the address, then I will show these
problems to the public for help.

Therefore, if any one who knows the address, please tell me.

I am learning using NUPOP software for email service, if you get
this message twice, I am sorry about that.

Many thanks.

Zhen Quan Liu

EM laboratory
Peking University
Beijing 100871
China

FAX ( 86 10 ) 256 1615

Email zqliu-at-pku.edu.cn




From: David C. Zawieja :      dcz-at-tam2000.tamu.edu
Date: Mon, 17 Jul 1995 08:53:09 -0500
Subject: Users of the Meridian Ultima

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Our group is considering upgrading an ACAS 570 non confocal microscope system to
the Meridian Ultima. We are primarily concerned about capability to do confocal
and photoactivatable caged compound studies. Before we plunk down the cost of
upgrading we would like to hear from any users of the Ultima about the pros and
cons of the system, particularly with regard to these types of uses.

I would also appreciate it if anyone could supply me with the address of the
confocal listgroup. Thanks!

Dave Zawieja
Assistant Professor
Dept. of Medical Physiology
Texas A&M Univ.- Health Science Center
College Station TX 77843
Phone:(409) 845-7465
FAX:(409) 847-8635
dcz-at-tamu.edu





From: spignole-at-ix.netcom.com (Susanne Brandom)
Date: Sun, 16 Jul 1995 21:24:33 -0700
Subject: Re: EDAX DX-4 and another legal & moral ???

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You wrote:
}
} I would like to know the email address of EDAX EDS service in USA,
} so that I can contact them. I have a DX-4 EDS here, and have some
} problems. If I cannot get the address, then I will show these
} problems to the public for help.
}
} Therefore, if any one knows the address, please tell me.
}
} Many thanks.
}
} Zhen Quan Liu
}
} EM laboratory
} Peking University
} Beijing 100871
} China
}
} FAX ( 86 10 ) 256 1615
}
} Email zqliu-at-pku.edu.cn
}
Let me first say that I am sorry that you are having problems and that
this must be a frustrating situation considering your location.

With regard to showing your problems to the public, please be adviced
that this is a published forum and therefore libel laws do apply. In
addition, by threatining to show problems to the public, your actions
could be interpreted to indicate malecious intent.

Second, in the same light that vendors should not use this forum as a
commercial vechicle, should customers use it as a mechanism for telling
the world about problems?

Is this another moral and legal issue for this listserver to consider?

Another approach might include asking if there are individuals that are
using the same equipment that could help through email. Then
communicate the problems to only those that are able to help.

Susanne Pignolet Brandom, Ph.D
MicroWorld Resources and News




From: sassaroli-at-msvax.mssm.edu
Date: Sat, 17 Jul 1993 12:54:53 -0400
Subject: Re: EDAX DX-4 and another legal & moral ???

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_________________________________
Massimo Sassaroli, D.Sc.
Dept. of Physiology & Biophysics
Box 1218
Mount Sinai School of Medicine
1 Gustave L. Levy Pl.
New York, NY 10029-6574

sassaroli-at-msvax.mssm.edu







From: sassaroli-at-msvax.mssm.edu
Date: Sat, 17 Jul 1993 12:46:09 -0400
Subject: Re: cryo on unfixed cells, A.P.Somlyo's reply

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"Dr. Andrew P. Somlyo" {aps2n-at-elvis.med.virginia.edu} offered this very
"illuminating" reply to what, at first sight, is a very thoughtful message
by Reinhard Rachel.
Does Dr. Somlyo suggest that the adverse effects of Freon on the atmosphere
demonstrated by a great number of scientific studies are "nonsense"? Or
that the remainder of Reinhard's message is "nonsense".
As far as I am concerned, this is yet another blatant example of bandwidth
abuse. I read most messages coming down this pipeline, even though many of
them are outside my field of interest. If I do not have anything
constructive to say, I usually keep my mouth shut and my fingers off the
keyboard and the reply button. Perhaps Dr. Somlyo could also learn to be
more sensitive and selective in his future replies!!

Sorry, but this sort of things get to me sometimes.

Sincerely,

Massimo Sassaroli

} This one-liner was the reply of Dr. Andrew P. Somlyo
}
} NONSENSE
}
} On Sat, 15 Jul 1995, Reinhard Rachel t4534 wrote:
}
} } I would not believe that there are still some people on this
} } earth using Freon or similar compounds, if I had not read
} } this e-mail. Freon should not be used any more, at all.
} } Liquid Propane or Ethane do the same job.
} } BUT: even immersing in Freon, Propan, or Ethan is not good enough
} } to freeze a sample with a thickness of more than a few microns without
} } damaging it. Only high pressure freezing will do it (up to a thickness
} } of 200 to 500 micron; see papers by M.Mueller et al. Zuerich).
} } If you can't do this, you will have to fix your tissue chemically in
} } advance and possibly cryo-protect it before freezing. This MAY
} } POSSIBLY give you a proper ultrastructure, depending on your
} } tissue or cells, if you are lucky.
} } Without this, your sample will be damaged during freezing and
} } substitution. --- Best regards,
} }
} } - - Reinhard Rachel {Reinhard.Rachel-at-biologie.uni-regensburg.de}
} } | )| ) Universitaet Regensburg, Lehrstuhl fuer Mikrobiologie
} } | \| \ D - 93040 Regensburg (Tel.: xx49-941-943-4534)
} }
} }

_________________________________
Massimo Sassaroli, D.Sc.
Dept. of Physiology & Biophysics
Box 1218
Mount Sinai School of Medicine
1 Gustave L. Levy Pl.
New York, NY 10029-6574

sassaroli-at-msvax.mssm.edu







From: William R. Oliver :      oliver-at-ipas.afip.mil
Date: Mon, 17 Jul 1995 13:17:45 -0500 (EST)
Subject: Re: EDAX DX-4 and another legal & moral ???

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On Sun, 16 Jul 1995, Susanne Brandom wrote:

} You wrote:
} Let me first say that I am sorry that you are having problems and that
} this must be a frustrating situation considering your location.
}
} With regard to showing your problems to the public, please be adviced
} that this is a published forum and therefore libel laws do apply. In
} addition, by threatining to show problems to the public, your actions
} could be interpreted to indicate malecious intent.
}
} Second, in the same light that vendors should not use this forum as a
} commercial vechicle, should customers use it as a mechanism for telling
} the world about problems?
}
} Is this another moral and legal issue for this listserver to consider?
}
} Another approach might include asking if there are individuals that are
} using the same equipment that could help through email. Then
} communicate the problems to only those that are able to help.
}
} Susanne Pignolet Brandom, Ph.D
} MicroWorld Resources and News
}


Ahem. One of the purposes of the listerver *is* to get folk
talking together to help each other with problems. If a company
is so paranoid about its products that they try to censor their
customers on the net, then they certainly deserve any bad press
they get from it.

Having a problem with a product and letting folk know about
it is not libel by any stretch of the imagination. Asking
for help with a solution is a reasonable thing to do, and
carries no negative ethical connotations.

On the other hand, the idea of trying to gag customers *does*
lead to some interesting ethical questions, I think.

billo




From: bozzola-at-siu.edu (John. J. Bozzola)
Date: Mon, 17 Jul 1995 13:27:13 -0600
Subject: Re: EDAX DX-4 and another legal & moral ???

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X-Sender: bozzola-at-saluki-mail.fiber2.siu.edu
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Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

On Sun, 16 Jul 1995, Susanne Brandom wrote:

} With regard to showing your problems to the public, please be adviced
} that this is a published forum and therefore libel laws do apply. In
} addition, by threatining to show problems to the public, your actions
} could be interpreted to indicate malecious intent.
}
} Second, in the same light that vendors should not use this forum as a
} commercial vechicle, should customers use it as a mechanism for telling
} the world about problems?
}
} Is this another moral and legal issue for this listserver to consider?

=========================

I thought that the purpose of the listserver was to share information about
techniques and products. I can tell you from personal experience that it is
very important to share such information - ESPECIALLY regarding equipment
and products. As long as one presents the facts and allows the other side
to respond (which, of course, we do) then ethical vendors should have
nothing to fear. I know that I would have probably selected a different
vendor for a recent purchase that we made had I more information.

On the other hand, one seldom writes letters to praise a job well done or a
good product. Maybe we need some good press too.

Peace.




#############################################################################
John J. Bozzola, Director
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
#############################################################################






From: M.V. Parthasarathy :      mvp2-at-cornell.edu
Date: Mon, 17 Jul 1995 14:33:58 -0400 (EDT)
Subject: Re: Reinhard Rachel's comments on cryofixation

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X-NUPop-Charset: English

Having had quite a bit of experience in various methods of cryofixation, I
can only say that Reinhard Rachel is right on target.

*************************************************************************
M.V. Parthasarathy
Professor of Plant Biology & Director, EM Facility
Section of Plant Biology
228 Plant Science Building
Cornell University, Ithaca, NY 14850
Telephone: (607) 255-1734
Fax: (607) 255-5407
E-mail: mvp2-at-cornell.edu
***********************************************************************




From: wise-at-vaxa.cis.uwosh.edu
Date: Mon, 17 Jul 1995 14:49:39 +0000
Subject: Asbestos question

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To all,

The Merck Index lists two groups or asbestos: serpentines (such as
chrysotile) and amphiboles (anthophyllite, actinolite, tremolite,
crocidolite). Are all equally harmful or do their toxicity vary? In
particular, how does tremolite rank for toxicity?

Bob


Robert R. Wise, PhD
Director, UWO Electron Microscope Facility
Department of Biology
University of Wisconsin Oshkosh
Oshkosh, WI 54901
(414) 424-3404
(414) 424-1101 fax
wise-at-vaxa.cis.uwosh.edu






From: wise-at-vaxa.cis.uwosh.edu
Date: Mon, 17 Jul 1995 14:49:39 +0000
Subject: Asbestos question

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To all,

The Merck Index lists two groups or asbestos: serpentines (such as
chrysotile) and amphiboles (anthophyllite, actinolite, tremolite,
crocidolite). Are all equally harmful or do their toxicity vary? In
particular, how does tremolite rank for toxicity?

Bob


Robert R. Wise, PhD
Director, UWO Electron Microscope Facility
Department of Biology
University of Wisconsin Oshkosh
Oshkosh, WI 54901
(414) 424-3404
(414) 424-1101 fax
wise-at-vaxa.cis.uwosh.edu







From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-aaem.amc.anl.gov
Date: Mon, 17 Jul 1995 15:22:36 -0500 (CDT)
Subject: EDAX DX-4 and another legal & moral ???

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G'day Subscribers....

Not to over react, but the original posting on
this subject appeared (at least to me) as a request
for information about how to contact EDAX by Email.

The rest I would attribute to a language problem as
the originator of the message was from China. I
do not think that the message was sent as a "threat"
but rather a poorly worded statement saying that

"If I can't get hold of EDAX then I will ask for
help in the public forum. "

Let's not go overboard, I think that Zhen Quan Liu
was simply asking for information.

To help Zhen Quan Liu I have forwarded the Email
message to EDAX by FAX and asked them to contact him.
At present EDAX does not have a "general" EMAIL address
but I understand that many of their service reps have
individual accounts.

Cheers... Nestor

Your Friendly Neighborhood SysOp.







From: Doug Davis :      doug_davis-at-maillink.berkeley.edu
Date: 17 Jul 1995 16:38:06 -0700
Subject: RE-Asbestos question

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Subject: Time: 4:31 PM
OFFICE MEMO RE:Asbestos question Date: 7/17/95

It's been a long time since I did asbestos quantitation but my gut feeling
about the undesirable physiological effects of asbestos fibers in the alveoli
of the lungs could be due to ends of the fibers puncturing the cells and
allowing other toxins to enter the cells by capillary action on the fiber
surface. As I recall, some forms of the fibers have a central channel or
groove that could facilitate this effect.

Doug Davis
EML Berkeley
26 Giannini Hall
Berkeley CA 94720






From: William R. Oliver :      oliver-at-ipas.afip.mil
Date: Mon, 17 Jul 1995 21:35:38 -0500 (EST)
Subject: Re: Asbestos question

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On Mon, 17 Jul 1995 wise-at-vaxa.cis.uwosh.edu wrote:

} To all,
}
} The Merck Index lists two groups or asbestos: serpentines (such as
} chrysotile) and amphiboles (anthophyllite, actinolite, tremolite,
} crocidolite). Are all equally harmful or do their toxicity vary? In
} particular, how does tremolite rank for toxicity?
}

Here's an excerpt from "Pathology of Occupational Lung Disease" by
Churg&Green:

begin excerpt

Fiber type, fiber size, and fiber aspect (length to width) ratio all
appear to play a role in disease. There is fairly clearcut evidence that
amphiboles are more dangerous than chrysotile in the genesis of human
mesotheliomas; the importance of fiber type in regard to asbestosis and
lung cancer is unclear.

Some of the earliest animal studies of asbestosis suggested that long
fibers produced more disease than short ones, and this observation has
been repeatedly demonstrated for both nonneoplastic lesions and
mesothelioma. The effects of short fibers.. is disputed. One point of
view suggests that short fibers have no or minimal effects; the other
suggests that, although the effects of individual short fibers may be less
than individual long fibers, larger numbers of short fibers may be just as
important as smaller numbers of long fibers...

... The effect of aspect ratio is similarly uncertain...

end excerpt

billo




From: spignole-at-ix.netcom.com (Susanne Brandom)
Date: Mon, 17 Jul 1995 20:37:20 -0700
Subject: An apology to all

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I am sorry if I misinterpreted the posting from Zhen Quan Liu.

My intention was not to threaten or to intimatdate, but to caution. I
realize that this was a mistake and an over reaction on my part. I
hope that my actions will be excused.

This forum provides a great mechanism for communicating with other
microscopists and again I am very sorry for causing a disruption.

Susanne Pignolet Brandom

All acts of stupidity are my own as are my opinions




From: David Dryden :      djd-at-electron.ph.unimelb.edu.au
Date: Tue, 18 Jul 1995 14:44:59 +1000
Subject: test only

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THIS IS A TEST ONLY {SORRY} .





From: oisydney-at-ozemail.com.au (Julie Sheffield-Parker)
Date: Tue, 18 Jul 1995 16:01:37 +1000
Subject: Microanalysis of hair and bone samples.

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Is it possible to identify accumulations of pollutants, heavy metals etc. in
bone and hair material by x-ray microanalysis i.e. are the elements ever
present in sufficient concentration to detect variations? If anyone has any
experience of this, please would they comment and send me any references
that they think may be useful. Thank you.



*************************************************
From:-

Julie Sheffield-Parker,
Oxford Instruments Pty. Ltd.,
P. O. Box 7,
Pennant Hills,
NSW 2120,
Sydney, AUSTRALIA

Tel: ++ 61 2 484 6108
Fax: ++ 61 2 484 1667
E-Mail: oisydney-at-ozemail.com.au

*************************************************





From: oisydney-at-ozemail.com.au (Julie Sheffield-Parker)
Date: Tue, 18 Jul 1995 20:48:42 +1000
Subject: Microanalysis of hair and bone samples.

Contents Retrieved from Microscopy Listserver Archives
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Is it possible to identify accumulations of pollutants, heavy metals etc in
bone and hair material by x-ray microanalysis, i.e. are the elements ever
present in sufficient concentrations to detect variations? If anyone has any
experience of this, please would they comment and send me any references
that they think might be useful. Thank you.


**************************************

From:

Julie Sheffield-Parker,
Oxford Instruments PTY Ltd.,
P. O. Box 7,
Pennant Hills,
NSW 2120,
Sydney, AUSTRALIA.

Tel: ++ 61 2 484 6108
Fax: ++ 61 2 484 1667
E-Mail: oisydney-at-ozemail.com.au

**************************************





From: kris-at-miat0.vein.hu (Kris Kovacs)
Date: Tue, 18 Jul 1995 13:44:19 -0500
Subject: Microanalysis of hair and bone samples.

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} Date: Tue, 18 Jul 1995 16:01:37 +1000
} X-Sender: oisydney-at-ozemail.com.au
} To: Microscopy-at-aaem.amc.anl.gov
} From: oisydney-at-ozemail.com.au (Julie Sheffield-Parker)
} Subject: Microanalysis of hair and bone samples.
}
} Is it possible to identify accumulations of pollutants, heavy metals etc. in
} bone and hair material by x-ray microanalysis i.e. are the elements ever
} present in sufficient concentration to detect variations? If anyone has any
} experience of this, please would they comment and send me any references
} that they think may be useful. Thank you.
}
}
}
} *************************************************
} From:-
}
} Julie Sheffield-Parker,
} Oxford Instruments Pty. Ltd.,
} P. O. Box 7,
} Pennant Hills,
} NSW 2120,
} Sydney, AUSTRALIA
}
} Tel: ++ 61 2 484 6108
} Fax: ++ 61 2 484 1667
} E-Mail: oisydney-at-ozemail.com.au
}
} *************************************************



Hi Julie,

I would suggest to try to carefully incinerate the hair and/or bone samples
and then analyze the ash residue. This way you will increase the the
concentration of contaminants to a measurable level. The incineration can be
performed at about 900 deg C in an ordinary laboratory furnace with sample
put into a platinum crucible covered with a platinum lid. By precisely
weighing the sample before and after incineration you can even quantify. I
actually did this sort of analysis with foodstuff as well as spices and
obtained good and reliable results.

Another possibility would be X-ray microfluorescence done in the SEM with a
special although simple attachment put onto the EDS detector. The X-ray
fluorescence will decrease significantly the detection limit although
quantitation is more difficult. Contact Dr. I. Pozsgai who is an expert in
this matter. He works for Research Institute for Technical Physics,
Budapest, Hungary. His e-mail address is as follows:

pozsgai-at-falcon.mufi.hu

Good luck, and contact me if you succeed!

Kris
Kristof KOVACS
University of Veszprem, Central Laboratory
P.O.Box 158, Veszprem, HUNGARY
H-8201
Phone: +36-(88)-421-684
Fax: +36-(88)-426-016





From: William Tivol :      tivol-at-wadsworth.org
Date: Tue, 18 Jul 1995 11:19:31 -0500 (EDT)
Subject: Re: Microanalysis of hair and bone samples.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} } Is it possible to identify accumulations of pollutants, heavy metals etc. in
} } bone and hair material by x-ray microanalysis i.e. are the elements ever
} } present in sufficient concentration to detect variations? If anyone has any
} } experience of this, please would they comment and send me any references
} } that they think may be useful. Thank you.
} }
} } Julie Sheffield-Parker,
}
} I would suggest to try to carefully incinerate the hair and/or bone samples
} and then analyze the ash residue. This way you will increase the the
} concentration of contaminants to a measurable level. The incineration can be
} performed at about 900 deg C in an ordinary laboratory furnace with sample
} put into a platinum crucible covered with a platinum lid. By precisely
} weighing the sample before and after incineration you can even quantify. I
} actually did this sort of analysis with foodstuff as well as spices and
} obtained good and reliable results.
}
} Another possibility would be X-ray microfluorescence done in the SEM with a
} special although simple attachment put onto the EDS detector. The X-ray
} fluorescence will decrease significantly the detection limit although
} quantitation is more difficult. Contact Dr. I. Pozsgai who is an expert in
} this matter. He works for Research Institute for Technical Physics,
} Budapest, Hungary. His e-mail address is as follows:
}
} pozsgai-at-falcon.mufi.hu
}
} Good luck, and contact me if you succeed!
}
} Kristof KOVACS
}
Dear Julie,
The advantage of x-ray microanalysis is that the elements of interest
can be localized to within a fraction of a micrometer (if the probe size is
small enough). Both sensitivity and quantitation are better with bulk methods
(atomic absorption, neutron activation, etc.). If you need to see the pattern
along a hair strand, or localize an element in bone, however, XMA is a good
technique. As to your original question, some pollutents may be present in
sufficient concentrations, but the sensitivity of XMA is only about a large
fraction of 1%, or a few millimolar. You will have to figure out whether
what you are interested in is that concentrated. If you are going to incinerate
the samples, do not use XMA--you have already lost the position information.
Good luck.
Yours,
Bill Tivol




From: GVKM07A-at-prodigy.com (DR CHARLES A GARBER)
Date: Tue, 18 Jul 1995 10:40:37 EDT
Subject: New WWW site

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-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --

Hello!

I am pleased to officially announce the "opening" of the SPI Supplies
"home page" on the WWW:

{http://mail.cccbi.chester.pa.us/spi/spihome.html}

No one in the "EM prep lab" business has ever attempted anything like
this before so please be patient as we develop it in a way that will be
both interesting as well as informative to anyone accessing the
information that is now "up" on our site.

This project represents our first steps toward the all-electronic SPI
Supplies "SourceBook" of products for the EM laboratory. We believe
that use of the new electronic media eventually will permit significant
reductions in the normally high marketing and distribution costs
associated with just about anything going into the EM laboratory,
leading to lower prices for our customers.

Any comments, suggestions, or other input you think might help us make
an even better "site" should be directed to "webmaster" at
{SpiSupp-at-aol.com} .


Chuck

======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail:
GVKM07A-at-prodigy.com
West Chester, PA 19381-0656 USA Customer Service:
SpiSupp-at-aol.com

########################################################
WWW: http://mail.cccbi.chester.pa.us/spi/spihome.html
########################################################
======================================================









From: GVKM07A-at-prodigy.com (DR CHARLES A GARBER)
Date: Tue, 18 Jul 1995 10:40:34 EDT
Subject: Microanalysis of hair/bone samples

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-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --

In response to the question of Julie Sheffield-Parker on July 18 about
the analysis of hair and bone samples for heavy metals, "pollutants",
etc., because of the always possible presence of metallo-organics,
which would be volatilized away at the temperatures of operation of the
typical muffle furnace, removal of the organics via "room temperature
ashing" is preferred since one eliminates the possibility of loss of
heavy metals.

Note: I am disclosing my own (possible) financial interest in this
reply, since our firm manufactures such plasma etchers (e.g. the SPI
Plasma Prep II). Now for the painful part: Other firms manufacturing
"room temperature" ashing equipment would include a) Denton Vacuum,
Inc. and b) Fisons VG Microtech.


Chuck

======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail:
GVKM07A-at-prodigy.com
West Chester, PA 19381-0656 USA Customer Service:
SpiSupp-at-aol.com

########################################################
WWW: http://mail.cccbi.chester.pa.us/spi/spihome.html
########################################################
======================================================





From: Manny Olds :      mao-at-access.digex.net
Date: Tue, 18 Jul 1995 12:50:32 -0400 (EDT)
Subject: Re: Microanalysis of hair and bone samples.

Contents Retrieved from Microscopy Listserver Archives
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Could you use bulk methods on subsections of a hair strand or on
collections of hair strands?

For example, if you have the follicles, you could chop up all the strands
of hair and collect all the segments "follicle to 1 cm", "1 cm to 2 cm",
etc. Then use the more sensitive methods to characterize the exposure to
pollutants over time.

You could get more finely spaced data by chopping the hairs up into
smaller segments, but that would reduce the total amount in any one batch.

- - - - - - - - - - - - - - - - - - - - - - - - - - - - -
Manny Olds (mao-at-access.digex.net)
Quality Systems Division, US Government Printing Office
vox +1-202-512-0767, fax +1-202-512-0773
- - - - - - - - - - - - - - - - - - - - - - - - - - - - -


On Tue, 18 Jul 1995, William Tivol wrote:

} } } Is it possible to identify accumulations of pollutants, heavy metals etc. in
} } } bone and hair material by x-ray microanalysis i.e. are the elements ever
} } } present in sufficient concentration to detect variations? If anyone has any
} } } experience of this, please would they comment and send me any references
} } } that they think may be useful. Thank you.
} } }
} } } Julie Sheffield-Parker,
} }
} } I would suggest to try to carefully incinerate the hair and/or bone samples
} } and then analyze the ash residue. This way you will increase the the
} } concentration of contaminants to a measurable level. ...

} } Kristof KOVACS
} }
} Dear Julie,
} The advantage of x-ray microanalysis is that the elements of interest
} can be localized to within a fraction of a micrometer (if the probe size is
} small enough). Both sensitivity and quantitation are better with bulk methods
} (atomic absorption, neutron activation, etc.).
} Bill Tivol
}




From: jerry-at-biochem.dental.upenn.edu
Date: Tue, 18 Jul 1995 12:07:57 -0400
Subject: Freon 113

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Dear fellow subscribers to Microscopy ListServer,

We are having a problem locating a vendor for Freon 113. We have
recently depleted our stock which we obtained from Bio-Rad who no longer
carries this product. We have contacted Ted Pella, Inc.; Polaron and Energy
Beam Sciences and none carry Freon 113. Is this form of Freon no longer
available in the U.S.?
If anyone knows of a vendor who still supplies Freon 113, please
e-mail pertinent info. If this product is no longer available, what
possible alternatives can be used in the final stage of dehydration after
the ethanol drying series and before critical point drying?

Thanks in advance for any help--Gerald Harrison
U. of P. S.E.M. Lab
jerry-at-biochem.dental.upenn.edu





From: Jay Jerome :      jjerome-at-isnet.is.wfu.edu
Date: Tue, 18 Jul 1995 13:59:18 -0400 (EDT)
Subject: Re: Freon 113

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We use liquid CO2 quite successfully for CPD. Be sure it is very dry. We
have gas company heat dry tanks before filling and use a filter on the
line. With these precautions we get excellent results.

Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-isnet.is.wfu.edu *
**************************************************************

On Tue, 18 Jul 1995 jerry-at-biochem.dental.upenn.edu wrote:

} Dear fellow subscribers to Microscopy ListServer,
}
} We are having a problem locating a vendor for Freon 113. We have
} recently depleted our stock which we obtained from Bio-Rad who no longer
} carries this product. We have contacted Ted Pella, Inc.; Polaron and Energy
} Beam Sciences and none carry Freon 113. Is this form of Freon no longer
} available in the U.S.?
} If anyone knows of a vendor who still supplies Freon 113, please
} e-mail pertinent info. If this product is no longer available, what
} possible alternatives can be used in the final stage of dehydration after
} the ethanol drying series and before critical point drying?
}
} Thanks in advance for any help--Gerald Harrison
} U. of P. S.E.M. Lab
} jerry-at-biochem.dental.upenn.edu
}
}




From: jerry-at-biochem.dental.upenn.edu
Date: Tue, 18 Jul 1995 16:07:36 -0400
Subject: Freon/Thanks

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To Microscopy Listserver Subscribers,

Thanks for all the helpful advise. E.M.S. at (215)646-1566 still
has Freon 113 at $110.00 for a case of four (450 ml). However, since
several suggestions were to go straight from 100% EtOH to very dry CO2, we
will also try this.

Thanks again--Gerald Harrison





From: Crossman, Harold
Date: 7/18/95 11:44 AM
Subject: Re: Low-cost video input boa

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Message-Id: {n1406047702.76381-at-macmail7.lbl.gov}
"Harold Crossman" {Harold_Crossman-at-macmail7.lbl.gov}
X-Mailer: Mail*Link SMTP/QM 3.0.0

Reply to: RE} Low-cost video input board for SparcStation?

Harold,
I passed your inquiry on to our imaging/computing group, and they came back with --

Vendor: Engineering Design Team
Product: S1V board
price: about $1,200
phone: 503-690-1234

Mike O'Keefe
Acting Head
NCEM, LBNL
(510) 486-4610

--------------------------------------

Greetings,

Does anyone know of a source for low-cost ( {$1500)
video input (RS-170 or slow scan) for a SUN
SparcStation 5? Software for same?

Harold J. Crossman
OSRAM SYLVANIA INC.
Lighting Research Center
71 Cherry Hill Drive
Beverly, MA 01915






From: Tina Carvalho :      tina-at-halia.pbrc.Hawaii.Edu
Date: Tue, 18 Jul 1995 15:07:58 -1000 (HST)
Subject: Microscopy in Omaha, Nebraska?

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Aloha! A colleague here in Honolulu will be moving to Omaha in
December, and will be looking for a job in EM or histology. She asks if
there is a local microscopy society there that she can hook up with-?
She figures she can call hospitals, universities, etc. cold (she called
me cold and we found her a nice EM job), but she would like to get in
touch with other like-minded microscopists.

Mahalo in advance,
Tina

Tina Weatherby Carvalho
Biological EM Facility
University of Hawaii
tina-at-ahi.pbrc.hawaii.edu





From: Walt Bobrowski :      bobroww-at-aa.wl.com
Date: Wed, 19 Jul 1995 10:38:22 -0400 (EDT)
Subject: Optimal Etching Time

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Mr-Received: by mta PETVAX.MUAS; Relayed; Wed, 19 Jul 1995 10:38:22 -0400
Mr-Received: by mta PETVAX; Relayed; Wed, 19 Jul 1995 10:38:24 -0400
Mr-Received: by mta SRVR01; Relayed; Wed, 19 Jul 1995 10:40:33 -0400
Disclose-Recipients: prohibited

I would appreciate colleagues comments regarding what they have found to be an
optimal etching time for sodium ethoxide on resin sections prior to LM
immunogold labelling. Thanks in advance.

Walt Bobrowski
Parke-Davis Research
Ann Arbor MI






From: Richard Edelmann :      EDELMARE-at-CASMAIL.MUOHIO.EDU
Date: Wed, 19 Jul 1995 11:44:44 -500
Subject: Silicone Diff. Pump Oil !!!

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Message-Id: {199507191457.HAA22509-at-holonet.net}

General question for the list.

I just discovered that our Denton Vacuum evaporator has been running
on silicone diff pump oil. My question is does anyone have any good
recomenndations for cleaning out ALL the silicone oil so we can
replace it with Santovac 5?

Thanks.





From: STEVEN E SLAP :      75767.640-at-compuserve.com
Date: 19 Jul 95 13:11:36 EDT
Subject: Re: Microscopy in Omaha, Nebraska?

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Tina-
Nebraska is part of the Central States Microscopy Society. The Secretary to
contact is Sue Jaconis, 11444 Lackland Road, St. Louis, MO 63146. In my 1994
membership list, I see 3 members from Omaha, two at St. Joseph Hospital and one
at Creighton University. I'll send you these names if you'd like.
Steven Slap





From: Meiser, Mark -MJME
Date: 1995-07-19 08:42
Subject: Subscribe

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Message-Id: {CPLAN276.MJME.911553080095200FCPLAN276-at-ION.CHEVRON.COM}



------------------------------------------------------------------------------



Please add me to the microscopy mail server.
My E-mail address is mjme-at-chevron.com. Thanks.





From: Katherine.S.Connolly-at-Dartmouth.EDU (Katherine S. Connolly)
Date: 19 Jul 95 11:01:37 EDT
Subject: Subscribe

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Message-id: {17690419-at-dancer.Dartmouth.EDU}

I gather this is the correct place to:

subscribe
Katherine S. Connolly-at-Dartmouth.edu




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-aaem.amc.anl.gov
Date: Wed, 19 Jul 1995 11:58:59 -0500 (CDT)
Subject: Local Area Societies

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For those of you that have access to the WWW, there is a complete
listing of all LAS (Local Affiliate Societies) associated with MSA.
Contact names & addresses are provided. Just scroll through the list.

The URL is: http://www.amc.anl.gov

then look for information on the Microscopy Society of America
and further down the hot list will be the LAS information.

I saw nothing listed in the Omaha area, but there was a
listing for Societies in Iowa, Oklahoma, and "Mountain States".


Nestor....

Your Friendly Neighborhood SysOp




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-aaem.amc.anl.gov
Date: Wed, 19 Jul 1995 12:01:23 -0500 (CDT)
Subject: Email from China & a Thank You Note

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From: zhenquan liu :      zqliu-at-pku.edu.cn
Date: Tue, 18 Jul 1995 22:01:36 -0600 (CST)
Subject: DX4

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X-Nupop-Charset: English


Dear Nestor,

I would like to say thanks to all those people taking care
of my previous email, and most of them have provided the address and
FAX number of EDAX in USA and the person's name whom I might contact
with.

Email is a very convenient way for communication and discussion in
the field of EM. It is new to most Chinese people in China, even in
universities. Also it is quicker and cheaper. The cost of one page
of FAX in Beijing is equal to 1/6 of my one month wage. That is
why I prefer email.

What I wanted to discuss with EDAX is pure scientific and technical.
It is obvious that my poor English did confuse some people, I do
apologize here for that. Anyway, I shall send EDAX a fax, telling them
my technical and scientific problems.

Due to the problem of my local computer, I cannot get the email
addresses of those people who wrote to me, so that I cannot
answer or say thanks to them individually.
They are:

Don Grimes, Yi Feng,
Babara Hartman, Miami University,
Susanne Brandom, Sid Patel,
X. G. Ning, Z. P. Wang,
Sender from Australia

I hope this email will make things clear.

If you think this mail is suitable, please transfer it to "microscopy",
otherwise please return to me.

Many thanks

Zhen Quan Liu

Peking University
Beijing, China
Email zqliu-at-pku.edu.cn




From: tphillips-at-biosci.mbp.missouri.edu (Tom Phillips)
Date: Wed, 19 Jul 1995 16:00:59 -0600
Subject: TEM of bacteriophage

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One of my colleagues would needs a TEM of a negatively stained phage prep.
Can anyone point me to a recent reference? TIA.


Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility
3 Tucker Hall
University of Missouri
Columbia, MO 65211
(314)-882-4712 (voice)
(314)-882-0123 (fax)






From: Ilene Sugino :      suginoik-at-UMDNJ.EDU
Date: Wed, 19 Jul 1995 17:05:06 -0400 (EDT)
Subject: job openings

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The Department of Ophthalmology at UMDNJ, New Jersey Medical School in
Newark has two technical positions open:
ELECTRON MICROSCOPIST: This position requires a bachelor's
degree plus a minimum of 3 years relevant experience. We are seeking a
highly skilled and motivated individual to perform research on the eye
who can perform all aspects of transmission and scanning electron
microscopy. Animal work will include euthanasia and tissue harvesting.
Individual should have excellent darkroom skills. Knowledge of
MacIntosh, image analysis, ICC and ISH highly desirable.
RESEARCH TEACHING SPECIALIST: This position requires a
bachelor's degree plus a minimum of 1 year relevant experience. We are
seeking a histologist with experience embedding and sectioning plastic
embedded tissue. Animal work is required. Knowledge of PC and Mac
computers and darkroom highly desirable.

If interested, please send your resume to
Ilene Sugino
UMDNJ-Ophthalmology
DOC 6th Floor
90 Bergen Street
Newark, NJ 07103
or fax me at 201 982-7762




From: INGRAM-at-RTI.ORG
Date: Wed, 19 Jul 1995 17:39:41 -0400 (EDT)
Subject: Freezing methods

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I thought this response from Andrew Somlyo was worth sharing...........amongst
other things, it does point out the need for care when using ethane or propane.
re Regensburg's message:
1. It ignores an extensive literature dealing with depth of ice-
crystal-free freezing as a function of coolant, plunge-velocity, etc. and
metal mirror properties, while operating at atmospheric pressure (e.g.: Bald
J. Microscopy 140: Pt.1 17-40, 1985 and refs. therein) .
2. Worse, the message ignores extensive experimental evidence showing
ice-crystal-free (even vitreous) freezing at atmospheric pressure,
albeit to a limited specimen depth. ( For muscle, a more "difficult" specimen
then, say, liver,see e.g.: Somlyo A.V. et al: J. Cell Biol. 74:828-857, 1977;
ibid 90:577-594, 1981; Padron et al; J. Microscopy 151 Pt.2 81-102,1988;
Sosa et al; Biophys. J. 67:283-292, 1994).
3. Advocacy of explosive coolants without an accompanying warning of
its potential dangers ( e.g.: Ryan and Liddicoat J. Microscopy 147:
337-340, 1987), true, is not nonsense, but worse. Electrical discharge dur
ing operation of a stimulator can cause far more damage than the
minute amount of Freon released into the atmosphere.
4. The uninformed use of the term "damage" can be misleading. As also
noted by Peter Ingram, high pressure can cause more "damage" to sub-
cellular composition than freezing at atmospheric pressure.
5. I want to protect the environment, but, as an American, I also
believe in the separation of church and state. Therefore, until someone provides


quantitative evidence to show that the amounts of Freon released by vast
hordes of cryo-electronmicroscopists have a SIGNIFICANT effect compared to,
for example, bovine eructations, I consider the LEGAL use of Freon to be a
matter of individual choice, not to be subject to the dictates of , often
uninformed ( see also the Shell-Atlantic fiasco) devotees of the Green
religion.


Hope this 'clears the air' as it were!

Peter Ingram
MAIL
SEND






From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 19 Jul 1995 17:50:53 -0400
Subject: RE-Cleaning Silicone Oils

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Message-ID: {n1405946197.97339-at-mse.engin.umich.edu}

Subject: Time: 5:22 PM
OFFICE MEMO RE:Cleaning Silicone Oils Date: 7/19/95

I investigated the matter of how to remove silicone oils from vacuum
apparatus rather thoroughly while writing the section on Diffusion Pump Oils
in my book on "Vacuum Methods in Electron Microscopy". Here is what I was
able to come up with (p. 185): "Silicone oils themselves are also very
difficult to remove from the parts of an electron microscope because they are
so viscous and insoluble. Some instrument manufacturers have refused to
issue service warranties on instruments if silicone oils are used in the
diffusion p[umps. The cleaning procedure recommended by the Dow Corning
Corporation (I called them and discussed the matter personally with one of
their engineers) consists simply of wiping away as much of the oil as
possible with a dry cloth or tissue, followed by repeated wiping with cloth
pads moistened with toluene, xylene, trichloroethylene, or
perchloroethylene." In addition to the above solvents, kerosene is
sometimes recommended. Repeated rinsing with one or mopre of these solvents
may ultimately get your system free enough of the old oil for your purposes.
As mentioned on pp. 188-190 of my book, You may want to check with the
manufacturer of the pump before changing the type of oil you use in it, to
be sure Santovac will work in it satisfactorily. If it was designed for use
with DC-704 silicone fluid, it's heater may not put out enough heat to give a
suitable rate of boiling with Santovac. If it uses DC-705, this will
probably not be a problem.
P.S. you can order my book from Portland Press, Ashgate Publishers, Old
Post Road, Brookfield, VT 05036-9704 (Ph: 800-535-9544) - it contains loads
of just this kind of practical information.





From: Scott Ashkenaz :      s_ashken-at-qm-japan.kla.com
Date: 20 Jul 1995 15:26:11 U
Subject: None

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Message-Id: {n1405868415.55670-at-qm-japan.kla.com}

subscribe

None




From: SveEn-at-pai.liu.se (Sverker Enestrom)
Date: Thu, 20 Jul 1995 08:41:37 +0200
Subject: Re: Optimal Etching Time

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} I would appreciate colleagues comments regarding what they have found to be an
} optimal etching time for sodium ethoxide on resin sections prior to LM
} immunogold labelling. Thanks in advance.
}
} Walt Bobrowski
} Parke-Davis Research
} Ann Arbor MI

For immunoEM you only need to *oxidize* the thin section using hydrogen
peroxide or
periodic acid. We routinely use 10% hydrogen peroxide for 10 min. This step
is of
course only necessary after embedding in epoxy resins. It is more easy and
efficient to
embedd in Lowicryls or Unicryl for immunoEM. *Etching* is performed if you
want to
stain the semithin epoxy resin sections. We use sodium ethoxide + toluene
(2:1) for
3-5 min.
If you need more information, feel free to contact me.

==================================================================
Sverker Enestrom M.D., Ph.D.
Department of Pathology
University of Linkoping, Sweden
Phone: +46 13 22 15 20
Fax: +46 13 13 22 57
==================================================================






From: FRIEDA CHRISTIE :      f.christie-at-rbge.org.uk
Date: Thu, 20 Jul 1995 08:59:32 BST
Subject: Re: Optimal Etching Time

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To: Walt Bobrowski {bobroww-at-aa.wl.com}

I normally make up a saturated solution of ethanolic sodium hydroxide
several days before I need to use it. It is ready when the solution has
turned dark brown in colour and I have found that it's etching
properties get stronger with time. Slides holding semithin sections (0.5
micron) are immersed in etching solution for 1 hour before being
rinsed well in ethanol. Care should be taken to ensure that the slides
are completely covered in ethanolic sodium hydroxide to prevent the
formation of crystals.




From: tphillips-at-biosci.mbp.missouri.edu (Tom Phillips)
Date: Thu, 20 Jul 1995 08:52:56 -0600
Subject: TEM of bacteriophage - method needed

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Message-Id: {v01510102ac341d8e7137-at-[128.206.15.189]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

I apologize my earlier posting was poorly worded. I was looking for a
protocol so I could negatively stain a preparation of bacteriophage
prepared by one of my colleagues. Thanks to those who offered TEM's as
well as pointers to methods.


Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility
3 Tucker Hall
University of Missouri
Columbia, MO 65211
(314)-882-4712 (voice)
(314)-882-0123 (fax)






From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 20 Jul 1995 12:58:39 -0400
Subject: RE-silicone oils/more

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Message-ID: {n1405877320.39446-at-mse.engin.umich.edu}

Subject: Time: 12:42 PM
OFFICE MEMO RE:silicone oils/more Date: 7/20/95

I just remembered that, while writing my book on Vacuum Methods, I also ran
across a detergent that is advertised as being suitable for removing silicone
and other oils (p. 72). This is a product known as RBS-35 manufactured by the
Pierce Chemical Co., P.O. Box 117, Rockford, IL 61105 (Ph: 815-968-0747).
This stuff may be intended primarily for use on glassware, and so may require
treatment in a boiling solution, I just don't recall; however, you might
contact the manufacturer and see if they can suggest a method of using it on
a DP. Incidentally, there will be silicone oil all through the pumping line
and the hose attached to the DP, as well as inside the evaporator, and so
these parts will also have to be cleaned if you want to get the system
reasonably free of silicones. Best of luck! W. C. Bigelow
(bigelow-at-umich.edu)





From: Jeffrey.Shield-at-mse.utah.edu (Jeff Shield)
Date: Thu, 20 Jul 1995 11:06:44 -0600
Subject: EM: staining proteins with Au

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To all,

A student here is interested in looking at proteins with EM. How does one
go about this? How does one stain proteins with Au? If anyone can point us
toward a reference or offer advice, it would be greatly appreciated. I
apologize for such a basic inquiry, but I'm only a materials scientist, and
mostly a metallurgist at that.

Thanks
--------------------------------------------------------- U U
| Jeff Shield | U U
| Department of Materials Science and Engineering | U U U U
| University of Utah | U U U U
| Salt Lake City, UT 84112 | U U U U
| 801/581-3179 Fax: 801/581-4816 | UUUUU U
| | U U
--------------------------------------------------------- UUUUU
Of making many books there is no end, and much study wearies the body.
-Eccl 12:12





From: vickie-at-macc.wisc.edu
Date: Thu, 20 Jul 1995 15:11:19 -0600
Subject: ANNOUNCE: Directory of Microscopists on the Net

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Message-ID: {8D5E0E300179AEAA-at-ggpl.arsusda.gov}


The Integrated Microscopy Resource at the University of Wisconsin-Madison
is pleased to provide the "Directory of Microscopists on the Net." It's
available on the World Wide Web at

http://www.bocklabs.wisc.edu/imr/microscopists.html

A fill-in form allows you to submit your name, organization/institution,
email address, and research interests. A search engine allows keyword
search, or the whole list can be viewed.

Having just started, the directory is fairly small. We could use your
help in building up the directory and making it a useful resource that
facilitates communication and collaboration.

Sincerely,
Stephan Spencer
Integrated Microscopy Resource
University of Wisconsin-Madison
Web: http://www.bocklabs.wisc.edu/imr/imr.html
email: sspencer-at-rhino.bocklabs.wisc.edu






From: Steve Miller, IMI, Park Ridge, IL
Date: 20 Jul 95 18:03:49 EDT
Subject: Denton Vac. on Santovac 5

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Please be aware that changing from DC704 to Santovac 5 results in a lower
pressure in the stack, you will suffer some loss in pumping speed. More
important is that you lower the changeover point from roughing to backing.

With DC 704 the pressure is about 300mTorr so you can changeover at 150-200mTorr
with little consequence. The pressure with Santovac 5 was related to me to be
about 150mTorr so you must go to 50-100mTorr of roughing.

Call me if you have any questions, as I recall you have an auto system and the
resetting of the auto sensors must be done or problems will result.

Your friendly Denton Doctor

Phone 800-388-8801, Fax 708-696-2541





From: SveEn-at-pai.liu.se (Sverker Enestrom)
Date: Fri, 21 Jul 1995 08:18:05 +0200
Subject: Re: EM: staining proteins with Au

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} To all,
}
} A student here is interested in looking at proteins with EM. How does one
} go about this? How does one stain proteins with Au? If anyone can point us
} toward a reference or offer advice, it would be greatly appreciated. I
} apologize for such a basic inquiry, but I'm only a materials scientist, and
} mostly a metallurgist at that.
}
} Thanks
} --------------------------------------------------------- U U
} | Jeff Shield | U U
} | Department of Materials Science and Engineering | U U U U
} | University of Utah | U U U U
} | Salt Lake City, UT 84112 | U U U U
} | 801/581-3179 Fax: 801/581-4816 | UUUUU U
} | | U U
} --------------------------------------------------------- UUUUU
} Of making many books there is no end, and much study wearies the body.
} -Eccl 12:12

Hello Jeff,
A simple way to look at peptide preparations is by negative staining with 2%
uranyl acetate (UAc). Just put a drop of your suspension on formvar-coated
copper
grids and contrast with the uranyl acetate. If you want to discover a partic=
ular
peptide you can step to immunoEM. With specific antibodies or antisera
against the peptide you will be able to reveal it in TEM. Dilute the protein
suspension with a phosphate buffer + 0.1% (w/w) BSA. Place a small drop
on formvar-coated nickel grids for about 1 min. Dry and let the grid float
for 1 h on a drop of antiserum diluted in PBS-BSA. Wash many times for
5 min on drops of PBS and place them then for 1 h on a drop of
protein A-Au (5 or10 nm) or protein G-Au diluted 1:10 in PBS-BSA. Wash and
contrast with 2% UAc as above.
Good luck.
Sverker

=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=
=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=
=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D
Sverker Enestrom M.D., Ph.D.
Department of Pathology
University of Linkoping, Sweden
Phone: +46 13 22 15 20
=46ax: +46 13 13 22 57
=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=
=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=
=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D=$D






From: :      JSMIEJA-at-peach.ia.polsl.gliwice.pl
Date: Fri, 21 Jul 1995 15:27:18 MEST
Subject: Summer School on Morphological Image and Signal Processing

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Dear Sir/Madam

Please find enclosed Second Announcement of Summer School on
Morphological Image and Signal Processing being held in Zakopane,
Poland, Sept. 27-30. This message has been sent to several list, so
I am sorry if it happen to reach you more than once.

Best regards,

Jaroslaw Smieja
Organising Committee

-----------------------------------------------------------------
-----------------------------------------------------------------

Second Announccement

Summer School on Morphological Image and Signal Processing
Zakopane, Poland, September 27-20, 1995


Programmme Committee

J. Chojcan (Gliwice, Poland)
E. Dougherty (Rochester, USA)
M. Gabbouj (Tampere, Finland)
J. Goutsias (Baltimore, USA)
S. Marshall (Glasgow, UK)
W. Mokrzycki (Warsaw, Poland)
I. Pitas (Thessaloniki, Greece)
J. Serra (Fontainebleau, France)
F. Vajda (Budapest, Hungary)


Organising Committee

H. Heijmans (Amsterdam, The Netherlands) - Scientific Director
J. Smieja (Gliwice, Poland) Local Arrangements
V. Starovoitov (Minsk, Belarus)
K. Wojciechowski (Gliwice, Poland) - General Director


Invited Lecturers

J. Astola (Tampere, Finland)
H. Heijmans (Amsterdam, The Netherlands)
P. Jonker (Delft, The Netherlands)
M. Schmitt (Fontainebleau, France)
L. Vincent (Peabody, USA)



Venue

Zakopane is the most famous and attractive place in the Polish Tatra
Mountains. It is located near the Slovakian border within a short
distance from Krakow and Katowice.


Accommodation

Participants are awaited in the "Siwarna" rest house, Zakopane
-Koscielisko, 32 Sywarne St. The accommodation is arranged in single,
double and three- bedded rooms and is available from
Tuesday, September 26th (with supper on that day as the first meal)
to Saturday, September 30th (supper on that day included). Full
board is provided (three meals daily).
For extra payment the rest house offers various recreation
facilities (swimming pool, sauna, massage, solarium, well equipped
gym-hall, tennis court).


Registration fee

The registration fee of 150 ECU includes participation fee, full
board and accommodation costs, social events and school proceedings.
It should be paid before September 1st, in USD (equivalent of
150 ECU on the day of payment) to the following account

Bank Slaski I/O Gliwice
312679-0020016301

with annotation "Summer School Gl 1330"

The registration fee can be also paid after arrival, in cash.
In that case it will be increased to 170 ECU.

A small number of grants is available for young researchers
from Eastern European countries.


Registration

The registration desk in the lobby of "Siwarna" will be open on
Tuesday, September 26th, from 16:00 to 22:00 and on Wednesday,
September 27th, from 8:00 to 14:00.


Social Events

Banquet
Mountain trip/ Sightseeing tour (one day)

The days of social events will not be established until the beginning
of the Summer School, since mountain trip depends on weather
conditions.


Transport

There are regular train links with Zakopane from Warsaw, Wroclaw,
Krakow and Katowice (in which airports are located). The timetables
are available upon request under address given below. There are also
regular bus links between Zakopane and each of these cities.
To reach "Siwarna" from the bus or railway station you may take one
of a number of minibuses (price about 1 zloty), a taxi or a bus
to Koscielisko Valley. In the last case leave at Krzeptowki II stop
and continue on foot (about 2 km).


Currency

Two currencies are valid in Poland: new zloty and old zloty.
One new zloty equals ten thousand old zloties
All prices are usually given in new zloty or both new and old
zloties. USD exchange rate is about two and a half new zloties
for one USD.
Money can be exchanged in exchanged offices (marked "Kantor")
and banks.

Weather

Although in September weather in Zakopane is usually nice,
since it is situated in high mountains, participants should
be prepared for any kind of weather, including strong wind
and rain.


Further information

For more details, please contact

Mr Jaroslaw Smieja
Silesian Technical University,
Dept. of Automatic Control,
Akademicka 16
44-101 Gliwice,
POLAND

e-mail: smieja-at-ia.gliwice.edu.pl
fax: (+48)-32-371165




SCIENTIFIC PROGRAM

Day 1

08.30-09.20: General introduction, H. Heijmans
Mathematical morphology: first principles
H. Heijmans - lecture, part 1,

09.30-10.20: Mathematical morphology: first principles
part 2, H. Heijmans

10.20-10.50: coffee break

10.50-11.40: Morphological measurements and random models
M. Schmitt - lecture, part 1

11:50-12:40 Morphological measurements and random models
M. Schmitt - lecture, part 2

12.40-14.30: lunch break

14.30-15.20: Granulometries
Luc Vincent - lecture

15.30-16.20: Segmentation
Luc Vincent - lecture

16.50-18.10: 4 short talks + discussion

18:30-20:00: supper



Day 2

08.30-09.20: Morphological filters
lecture - part 1, H. Heijmans

09.30-10.20: Morphological filters
lecture - part 2, H. Heijmans

10.20-10.50: coffee break

10.50-11.40: Statistical properties and optimization of weighted
median filters
J. Astola - lecture, part 1

11.50-12.40: Statistical properties and optimization of weighted
median filters
J. Astola - lecture, part 2

12.40-14.30: lunch break

14.30-15.20: Software and hardware implementation of a 2D
morphological toolbox
P. Jonker - lecture, part 1

15.30-16.20: Software and hardware implementation of a 2D
morphological toolbox
P. Jonker - lecture, part 2

16.50-18.30: 5 short talks + discussion

18:30-20:00 supper


Day 3

08.30-09.20: Queues and priority queues for morphological
algorithms
Luc Vincent - lecture, part 1

09.30-10.20: Queues and priority queues for morphological
algorithms
Luc Vincent - lecture, part 2

10.20-10.50: coffee break

10.50-11.40: Implementation of a 3D morphological toolbox;
Extension to 4D and 5D to be applied in robot path
planning
P. Jonker - lecture, part 1

11.50-12.40: Implementation of a 3D morphological toolbox;
Extension to 4D and 5D to be applied in robot path
planning
P. Jonker - lecture, part 2

12.40-14.30: lunch break

14.30-15.20: Geodesy and digital distances
M. Schmitt - lecture, part 1

15.30-16.20: Geodesy and digital distances
M. Schmitt - lecture, part 2

16:50-18:10: 4 short talks + discussion

18:30-20:00: supper


Short talks (in alphabetical order):

T.Bayik, L.Akarun (Turkey)
Color Edge Detection Using Vector Order Statistic Filters

Z. Fazekas (Hungary)
Shape Description of Pathological Red Blood Cells Based on
Near-border Skeleton Points

N.R. Harvey, S. Marshall (UK)
Optimisation of Rank Order Morphological Filters Using Genetic
Algorithms

H.Huttunen, P.Koivisto, P.Kuosmanen, J.Astola (Finland)
Optimisation of Soft Morphological Filters under Structural
Constraints

Z. Kus (Poland)
Stack Filter Design Based on the Mean Absolute Error Criterion

F.Llorens, F.Escolano, J.A.Puchol, M.Pujol,J.M.Chamizo, R.Rizo (Spain)
Guidelines for Using Alternating Sequential Filters in Image
Restoration

S.Marshall, J.A.Bangham, A.Noble, J.M.Brady (UK)
Review of Mathematical Morphology Research in UK

A.Meyster, J.B.T.M. Roerdink (The Netherlands)
An Alternative Algorithm for Computing Watersheds on Shared Memory
Parallel Computers

F.Potjer, H. Heijmans (The Netherlands)
Some Aspects of Connected Morphological Operators

P.Strumillo, A.Materka (Poland)
Processing of Atrial Fibrillation ECG Signal Using Mathematical
Morphology and Artificial Neural Networks

V.Starovoitov (Belarus)
From Neighbourhood Structures to Local Low Level Image
Transformations

A.Tuzikov (Belarus)
Symmetry Measure Evaluation of Grey-Scale Images via Dilation

S. Warecki (Poland)
ASIC Pipelined Stack Processor





From: Tracy Vogrin :      tmv-at-jhunix.hcf.jhu.edu
Date: Fri, 21 Jul 1995 13:29:46 -0400 (EDT)
Subject: fresh-frozen specimen use in tem

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hi everyone--
i'm an undergrad just learning about this stuff, doing tem of the
cruciate ligaments of the knee. i'm having some trouble obtaining fresh
specimens in the right age range for my study and was wondering how
important it was to use never-frozen specimens, and use fresh-frozen
instead. i've heard that it's not the best thing to do but i can't seem
to find documentation on it. my goal is to measure collagen fiber
diameters, so if these would remain unchanged by the freezing, this would
be the best route for me.

any help would be greatly appreciated. if possible, please send replies
to me directly because i just subscribed to the server and am not sure if
i am on the mailing list yet. thanks in advance...

tracy vogrin
musculoskeletal research center
pittsburgh, pa




From: kim_rensing-at-mindlink.bc.ca (Kim and Cathy Rensing)
Date: Fri, 21 Jul 1995 12:07:09 -0700
Subject: Freeze Substitution media

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Message-Id: {m0sZNNY-0003UzC-at-rsoft.rsoft.bc.ca}
X-Sender: kim_rensing-at-mindlink.bc.ca (Unverified)
Mime-Version: 1.0
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Does anyone have experience using freeze substitution media other than the
standard acetone or alcohols? I am trying to immuno-localize a substance
which these readily extract. I have had a suggestion for n-butanol but I
have no information on the compatibility of this (or other media) with
substitution equipment or with various epoxies. Any advice would be
appreciated.

Thanks, Kim Rensing






From: Trevor Sewell :      SEWELL-at-uctvms.uct.ac.za
Date: Sat, 22 Jul 1995 10:49:13 +0200
Subject: EMSA/MAS format

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Dear All,

IS there a standard document describing the EMSA/MAS format
for EDX spectra? Is it on the network?
Many thanks
Trevor Sewell




From: hris-at-facstaff.wisc.edu (Hans Ris)
Date: Sun, 23 Jul 1995 17:23:54 -0500
Subject: epoxy extraction for LM and SEM

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From Hans Ris, hris-at-facstaff.wisc.edu, Integrated Microscopy Resource,
University of Wisconsin, Madison, WI. USA.
Walt Bobrowski and Sverker Enestrom commented recently on epon extraction
for LM and Immunogold labelling using sodium ethoxide. Sodium or Potassium
ethoxide or methoxide have been used in the past to remove epon from blocks
or sections for LM cytochemistry or SEM imaging. Experience has shown that
these agents are inefficient and highly destructive for cell structures.
In 1990 Iwadare et al, Stain Technol.65,205-209, published an improved
and less destructive protocol for LM cytochemistry and immunolabelling
(crown ether complex). Dr Marek Malecki and I have explored this new
technique for high resolution SEM imaging of thick epon sections after epon
removal. The results have been published in the Journal of Structural Biology
111, 148-157(1993). This new technique preserves nanometer structures in the
nuclear pore complex and insect flight muscle. After extraction of the epon
immunolabelling of actin, myosin and alpha-actinin was successful. The
extraction solution is commercially available. Source and details of
procedures are described in the J.Struct.Biol. 111,148 (1993) paper by
Ris and Malecki.









From: AMCGroup2-at-aol.com
Date: Mon, 24 Jul 1995 00:44:45 -0400
Subject: Specimen Preparation Workshops

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A large volume of messages relating to specimen preparation issues and
concerns are exchanged on this listserver on a steady basis. It is obvious
that any information on related training, service, or product resources are
much needed and appreciated by the subscribers.

I would like to inform those of you with materials science applications that
AMC Group offers advanced hands-on specimen preparation workshops
semiannually on a regular basis. The upcoming workshops for Fall `95 have
been scheduled as follows:

SEM/TEM SITE-SPECIFIC CROSS-SECTIONING
1. Wedge Polishing for TEM, Dallas, TX (Oct. 25-27)
2. Focused Ion-Beam (FIB) Milling, Hillsboro, OR (Nov. 1-3)
3. Microcleaving, Sunnyvale, CA (Nov. 6)

LM/SPM/SEM/TEM MATERIALS ULTRAMICROTOMY
***All to be held in Phoenix, AZ
1. UM for Surface Preparation (Oct. 16-17)
2. UM for Thin Section Preparation (Oct. 16-18)
3. Advanced UM (Oct. 19-20)

To receive a copy of the workshops brochure (including the registration form)
please contact us directly. Thank you.

Rene E. Nicholas
Business Manager
AMC Group
(602) 949-4203
FAX: (602) 947-7615
E-mail: AMCGroup2-at-aol.com





From: ddd-at-techunix.technion.ac.il (ddd)
Date: Mon, 24 Jul 1995 14:55:31 +0300
Subject: CUT MY UMBILICAL CORD PLEASE!!!

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Dear Listmaster,


Repeated attempts to unsubscribe have been futile! Please help!
I sent UNSUBSCRIBE messages each with one version of my e-mail address
ddd-at-tx.technion.ac.il or ddd-at-techunix.technion.ac.il
to the listserver LISTSERVER-at-AAEM.AMC.ANL.GOV but alas to no avail.
Sorry to bother all subscribers, but maybe its time to renew instructions
to list users.

thanks in advance

dd






From: jeanne_barker-at-merck.com (Jeanne Barker)
Date: 24 Jul 1995 08:17:07 U
Subject: Wicking off of PVA

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Message-Id: {9507241225.AA05910-at-igw.merck.com}

7/24/95
Wicking off of PVA 7:21 AM
Dear Microscopists,
Has anyone developed a method of wicking off PVA from grids in a consistent
manor, or found an alternative way of getting the excess PVA off the grids? I
have too much inconsistency. There must be a better way...
Thank-you,
Jeanne






From: Mr James Wesley-Smith :      WESLEYSM-at-biology.und.ac.za
Date: Mon, 24 Jul 1995 14:53:10 +0200 (SAST)
Subject: Re: Freeze Substitution media

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Message-ID: {MAILQUEUE-101.950724145309.568-at-biology.und.ac.za}
Microscopy-at-AAEM.AMC.ANL.GOV

} Date sent: Fri, 21 Jul 1995 12:07:09 -0700
} To: Microscopy-at-AAEM.AMC.ANL.GOV
} From: kim_rensing-at-mindlink.bc.ca (Kim and Cathy Rensing)
} Subject: Freeze Substitution media

} Does anyone have experience using freeze substitution media other than the
} standard acetone or alcohols? I am trying to immuno-localize a substance
} which these readily extract. I have had a suggestion for n-butanol but I
} have no information on the compatibility of this (or other media) with
} substitution equipment or with various epoxies. Any advice would be
} appreciated.
}
} Thanks, Kim Rensing
}
}
Dear Kim
Try the freeze-substitution medium of Kaeser (1989). This medium
relies on the ability of dimethoxy- propane to dissociate into
methanol and acetone in the presence of water. The medium is made up
of:

6 ml DMP (acidified with three drops of 0.1 N HCl in 25 ml DMP)
3 ml acetone
2.25 ml methanol
0.75 ml 10% uranyl acetate in methanol
0.6 ml 25% aqueous glutaraldehyde

The addition of 0.1 g of osmium is your call, as is the percentage of
glutaraldehyde recommended. The reference to this information is
Journal of Microscopy, Vol. 154, Part 3, pp 273-278 (1989).


Good luck.





James Wesley-Smith
Electron Microscope Unit
George Campbell Building
University of Natal
Durban, South Africa





From: P.V.Hatton :      P.V.Hatton-at-sheffield.ac.uk
Date: Mon, 24 Jul 1995 16:43:44 +0100
Subject: CS-Auto freeze-sub, any surplus?

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Dear Users,

The CS-Auto has been superceded by a new piece of equipment. Many
labs. with better funding than my own and greater need of
freeze-substitution have purchased the new equipment. Is it
therefore reasonable to try and get hold of a second hand CS Auto? I used one
once on loan and it was excellent (apart from the large quantities of
low viscosity resin that we spilt).

I hear a rumour that many laboratories have CS-Auto freeze
substitution equipment that has been made redundant by purchase of
the more modern system. If any one is interested in selling one to me
for minimal cost, please get in touch. Obviously a UK based
instrument would be easier to collect!

Best wishes, Paul

Dr Paul V. Hatton
Lecturer in Biomaterials
School of Clinical Dentistry
University of Sheffield
Claremont Crescent
SHEFFIELD S10 2TA

Tel. (0114) 271 7938
Fax. (0114) 2665326
or 2797050




From: cook-at-aaem.amc.anl.gov (Russell E. Cook)
Date: Mon, 24 Jul 1995 16:43:44 +0100
Subject: Re: EMSA/MAS format

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Message-Id: {v01510102ac39363c0410-at-[146.139.72.78]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
To: microscopy-at-aaem.amc.anl.gov

} Dear All,
}
} IS there a standard document describing the EMSA/MAS format
} for EDX spectra? Is it on the network?
} Many thanks
} Trevor Sewell

There is a document on the net which describes the MSA/MAS format, but a
more complete description of the format can be found in the EMSA Bulletin
21:2, pages 35-41, November, 1991. I looked at the on-line document this
AM and it needs some corrections. If you follow its instructions in
upper-case text, you should be OK. I will attempt to get a fully correct
version put into place later this week. The document can be obtained by
anonymous ftp from:

WWW.AMC.ANL.GOV/AMC-3/ANLSoftwareLibrary/2-EMMPDL/Xeds/Rwemmpdl/Rwemmpdl.doc .

The IP# for WWW.AMC.ANL.GOV is 146.139.72.10 .

Russell E. Cook
Electron Microscopy Center for Materials Research
Argonne National Laboratory
9700 South Cass Avenue
MSD-212
Argonne, IL 60439
(708)252-7194
FAX: (708)252-4798






From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-aaem.amc.anl.gov
Date: Mon, 24 Jul 1995 11:30:34 -0500 (CDT)
Subject: MSA/MAS File Format

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The documentation on the MSA/MAS File format is available
via Anonymous FTP from

Host: WWW.AMC.ANL.GOV

Username: Anonymous
Password: Your Email Address

Look in the Directory Structure Path

/ANLSoftwareLibrary/2-EMMPDL/Xeds/EMMFF

The abstract is called emmff.abs, the documentation
emmff.doc, the source code emmff.src

the file Emmff.Total contains all 3 files,

The subdirectories EMMFF.MAC, EMMFF.IBM contain
Mac and PC specific code and test programs.


Nestor
Your Friendly Neighborhood SysOp




From: Jaime Dant :      jaime-at-borcim.wustl.edu
Date: Mon, 24 Jul 1995 15:53:57 -0500
Subject: PVA Staining

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Dear whomever,

While writing a response for you, I hit some key that not only killed my current
document but my email listing as well.... Sorry I can't address you by name.

I process cryo electron microscopy almost daily. PVA/Ua staining is a matter
of routine in our lab and we rarely have a problem.

First we make wire loops out of 30 AWG wire (from an EM supplier) and stuff
the ends into micropipet tips. These can be used over and over again after
cleaning with H2O.

Then we use a stock of 2% PVA(aq) and 3% UA(aq) (foil covered) kept at 4C.

When ready we mix 900ul of PVA with 100ul of Ua in a syringe and push it
through a syringe filter onto ice chilled parafilm for staining (approx. 4 min)
Invert and float the grids to stain.

We pick the grids up with the wire loops and (here is the important step) CENTER
THE GRIDS WITH A FEW TAPS OF THE FINGER AND BOLT ALL OF THE STAIN FROM THE
CORNERS WITH FILTER PAPER (we cut small pie shaped wedges out of the paper).
That's blot not bolt.

Then stand the pipet tip up in a 96 well tissue culture plate. Or whatever.

Anyway, this method works very well and if you wish to ask me any questions,
feel free. I would be honored to help.

Jaime A. Dant
Washington University School of Medicine
Molecular Microbiology
St. Louis, MO 63110
jaime-at-borcim.wustl.edu
(314) 362-4987




From: smithj-at-acad.winthrop.edu
Date: Tue, 25 Jul 1995 09:00:26 -0400
Subject: LM/fluorescence/reducing background

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LM folk:
We are labelling the muscles in small animals with BODIPY/
phalloidin. It looks like we are going to need freeze-sub
to get good structural preservation (the animals contract
violently in fixatives, and don't anesthetize). Freeze-
sub appears to imply glutaraldehyde, which gives intense
background staining (not surprising). Does someone out
there have a favorite recipe for using Borohydride to reduce
background in Glutaraldehyde-fixed material for fluorescence
microscopy?
TIA
Julian Smith III
Biology
Winthrop University




From: colijn-at-kcgl1.eng.ohio-state.edu (Henk Colijn)
Date: Tue, 25 Jul 1995 10:28:12 -0400 (EDT)
Subject: Pd electropolishing difficulties

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We are trying to electropolish pure Pd and Pd/Ga alloys, but are having
difficulty with getting a smooth surface. So far (following the method of
Witcomb) we have been using
70% glacial acetic acid,
4% perchloric acid,
18% glycerol,
8% butyl cellosolve.

This stuff is *really* viscous at low temperature! We have found that we
don't get polishing at the 40 volts reported by Witcomb, but have had to go
higher (even to 80 volts!) During the polishing the electrolyte
temperature climbs from 3-4C up to 15C due to lack of heat transfer to the
ice bath.

Does anyone have any suggestions on improving our polishing procedure or on
keeping our electrolyte temperature down?

Thanks in advance,
Henk

Henk Colijn colijn.1-at-osu.edu OSU Campus Electron Optics Facility
-------------------------------------------------------------------
An optimist believes that we live in the best of all possible worlds.
A pessimist fears this is true.






From: rutledge phil :      prutle1-at-gl.umbc.edu
Date: Tue, 25 Jul 1995 11:43:38 -0400 (EDT)
Subject: TEM/SEM books

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Does anybody know of any good books for the processing of marine organisms
and tissues? There seems to be plenty of references for mammalian
tissues, viruses, bacteria, etc., but I am having problems finding a good
reference for doing TEM/SEM on salt and fresh water samples. I would also
like to know if there is an ultrastructural atlas available for these
types of samples.
If you know the publisher and ISBN number that would be a great help also.

Thanks in advance,

Phil 8-{)




From: chandler-at-lamar.ColoState.EDU (John Chandler)
Date: Tue, 25 Jul 1995 09:59:04 -0600
Subject: test

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test






From: jfmjfm-at-umich.edu (John F. Mansfield)
Date: Tue, 25 Jul 1995 13:15:47 -0400
Subject: Lecturer Position Open

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X-Sender: jfmjfm-at-srvr5.engin.umich.edu
Message-Id: {v02120d07ac3ad70ad55f-at-[141.213.21.13]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Do not reply to me, READ the entire posting before replying.

ADJUNCT LECTURER POSITION

THE UNIVERSITY OF MICHIGAN

The Department of Materials Science and Engineering at The University of
Michigan, Ann Arbor, is seeking candidates for a non-tenure track lecturer.
Responsibilities include: assisting or leading in the development of new
undergraduate teaching materials and methods that place a strong emphasis
on multimedia presentation techniques; the development of self-paced,
interactive learning modules; participation in the development and teaching
of new undergraduate laboratory modules that place a strong emphasis on
processing and physical and mechanical property characterization. The
department currently enrolls approximately 140 undergraduate students and
is an integrated materials program encompassing metals, ceramics, polymers
and EMO materials. Applicants must have a Ph.D. in materials science and a
demonstrated interest in undergraduate education. Minimum one year
appointment, renewable to three years based on funding and performance.
Send resume and list of references to:

Professor Albert F. Yee, Chairman
Department of Materials Science and Engineering
The University of Michigan
2300 Hayward Street
Ann Arbor, MI 48109-2136
or
afyee-at-engin.umich.edu

An Equal Opportunity Affirmative Action Employer




John Mansfield
North Campus Electron Microbeam Analysis Laboratory
413 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (313)936-3352 FAX (313)936-3352
jfmjfm-at-engin.umich.edu, John.F.Mansfield-at-umich.edu
or jfmjfm-at-umich.edu they all reach me!
URL: http://www.engin.umich.edu/~jfmjfm/jfmjfm.html






From: wise-at-vaxa.cis.uwosh.edu
Date: Tue, 25 Jul 1995 12:47:08 +0000
Subject: Asbestos question

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Thanks to all who responded to my (rather vague) question regarding
asbestos. I got a lot of good leads to follow up.

Bob


Robert R. Wise, PhD
Director, UWO Electron Microscope Facility
Department of Biology
University of Wisconsin Oshkosh
Oshkosh, WI 54901
(414) 424-3404
(414) 424-1101 fax
wise-at-vaxa.cis.uwosh.edu






From: wise-at-vaxa.cis.uwosh.edu
Date: Tue, 25 Jul 1995 12:50:57 +0000
Subject: Fixation of insects

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We are setting up to do TEM of caddis fly larvae. Does anyone have a good
protocol (or reference therefor) for TEM processing of insects? Will
fixatives get through the intact exoskeleton? Is there any way to dissect
them (to promote penetration of fixative) without ending up with a bunch of
squished insect goo?

Bob


Robert R. Wise, PhD
Director, UWO Electron Microscope Facility
Department of Biology
University of Wisconsin Oshkosh
Oshkosh, WI 54901
(414) 424-3404
(414) 424-1101 fax
wise-at-vaxa.cis.uwosh.edu






From: BGIAMMARA-at-gemini.mco.edu
Date: Tue, 25 Jul 1995 16:44:25 -0500 (EST)
Subject: Sorval MT-2B

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Hello... We have a new Chair for Microbiology who is interested
in obtaining a used Sorval MT-2B Ultramicrotome for one of his people who
trained on this instrument and desires one.
Please e-mail such availability, price and information to
gtcole-at-opus.mco.edu or call Dr. Garry Cole (419)381-5423.
Thanks. Appreciate your help.
Beverly Giammara
(419)381-4996




From: Gary Login :      glogin-at-bih.harvard.edu
Date: Tue, 25 Jul 1995 16:45:11 -0400
Subject: Re: Fixation of insects

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Microwave-accelerated chemical fixation methods have been used with success to
fix insects for TEM:

1. Lindley VA: A new procedure for handling impervious biological specimens.
Microsc Res Tech 1992, 21:355-360

2. Smid HM, Schooneveld H, Meerloo T: Microwave fixation of water-cooled insect
tissues for immunohistochemistry. Histochem J 1990, 22:313-320

3. contact me directly for detailed info.



In message writes:
} We are setting up to do TEM of caddis fly larvae. Does anyone have a good
} protocol (or reference therefor) for TEM processing of insects? Will
} fixatives get through the intact exoskeleton? Is there any way to dissect
} them (to promote penetration of fixative) without ending up with a bunch of
} squished insect goo?
}
} Bob
}
}
} Robert R. Wise, PhD
} Director, UWO Electron Microscope Facility
} Department of Biology
} University of Wisconsin Oshkosh
} Oshkosh, WI 54901
} (414) 424-3404
} (414) 424-1101 fax
} wise-at-vaxa.cis.uwosh.edu
}


Gary R. Login, D.M.D., D.M.Sc.
Assistant Professor of Oral Pathology
Beth Israel Hospital
Department of Pathology
330 Brookline Avenue
Boston, Massachusetts, 02215

glogin-at- bih.harvard.edu
Telephone: 617-667-2034
Fax: 617-667-8676





From: gwe-at-biotech.ufl.edu (Greg Erdos)
Date: Wed, 26 Jul 1995 09:35:56 GMT
Subject: Cytochem. Biol.

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Is anyone aware of a stain or cytochemical reaction to detect the presence
of iodine in tissue?
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
-at- Greg Erdos Phone: 904-392-1295
-at-
-at- Scientific Director,
-at-
-at- ICBR Electron Microscopy Core Lab
-at-
-at- 218 Carr Hall Fax 904-846-0251
-at-
-at- University of Florida E-mail: gwe-at-biotech.ufl.edu
-at-
-at- Gainesville, FL 32611
-at-
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-





From: Mnr HJ Els :      HJELS-at-op1.up.ac.za
Date: Wed, 26 Jul 1995 12:06:10 GMT+2
Subject: microwave courses

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A colleague of mine without access to the microscopy listserver
asked me to send out the following request. Any information on
forthcoming courses, workshops or meetings (1995/1996) on microwave
techniques in the UK, Europe, Australia or USA would be welcome.
The emphasis should be on electron microscopy - TEM of tissue
(human or animal) preferably in the field of pathology.
Thank you,
Hercules Els
EM Unit, Fac Vet Sci,
Univ of Pretoria
Onderstepoort
hjels-at-OP1.up.ac.za




From: jandt-at-msc.cornell.edu
Date: Wed, 26 Jul 1995 10:41:23 -0400 (EDT)
Subject: smooth polymer surface

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Reply-To: jandt-at-msc.cornell.edu


} Hi,
}
} I would like to "smoothen" a surface of a polystyrene bulk sample.
}
} The surface roughness should be in the range of nanometer (or better?).
}
} I think normal polishing methods do not apply here since they are
}
} to coarse.
}
} Does anyone have an idea of a good method that could help?
}
} Thanks very much in advance for your help.
}
} jandt-at-msc.cornell.edu
}





From: Paul Webster :      Paul.Webster-at-QuickMail.Yale.edu
Date: 26 Jul 1995 10:53:28 -0400
Subject: Microwave course

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Message-Id: {n1405366366.83782-at-QuickMail.Yale.edu}

Three day hands-on practical workshop for use of microwave ovens in electron
microscopy (with focus on 3 hr processing and embedding).

January 10 - 12 1996.

Center for Cell Imaging,
Yale University School of Medicine
Department of Cell Biology
333 Cedar Street
New Haven, CT 06520-8002
U.S.A.

Contact: Paul Webster.
(203) 785 3219






From: em-at-mediacity.com (Ed Monberg)
Date: Wed, 26 Jul 1995 11:06:15 -0800
Subject: Biotech Sale - get your checkbooks out and call before Friday

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Message-ID: {C62716300179AEAA-at-ggpl.arsusda.gov}

Dear List:

Quite an opportunity (unfortunately at someone's expense) is before you:

A company experiencing funding difficulties is selling a number of very high end Zeiss, Wild and Nikon scopes for both Cell and Animal work, as well as much other equipment involved with bio and recombinant studies.

Please address your Attention and inquiries to:

LESLIE FLINT or CAROLE KURAHARA
GenPharm International
297 North Bernardo Ave
Mountain View, CA 94043
415-964-7024
415-964-3537 (fax)


If you are serious abous some of their high end equipment, you may be able to persuade them to delay the sale deadline which is this Friday, or to conduct an auction
(perhaps on the net ?)

Regards to all,

Ed Monberg
LMDC
3101 Whipple Road
Union City, CA 94587-1216






From: STEVEN E SLAP :      75767.640-at-compuserve.com
Date: 26 Jul 95 14:24:56 EDT
Subject: Used MT-2B

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Microscopy Listserver {Microscopy-at-aaem.amc.anl.gov}

Dear Dr. Cole,
Beverly Giammara posted a message on the microscopy listserver that you are
looking for a used Sorval MT-2B ultramicrotome. I recommend that you contact
Bill McGee at Microtome Service Co. at (315)451-1404. Bill is a former Sorval
microtomy specialist who refurbishes and services microtomes. I confirmed with
Bill that he has an MT-2B in stock.
Best regards,
Steven Slap





From: m.dickson-at-unsw.EDU.AU (melvyn dickson)
Date: Thu, 27 Jul 1995 12:39:14
Subject: RE: HV cut-off in H-7000

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To: microscopy-at-aaem.amc.anl.gov

Hello out there fellow Hitachi H-7000 owners.
We have an intermittent problem which you might have encountered and may know
an answer to.

Intermittently- particularly if the microscope has been (pumping but) unused,
operating the photo lever to take a film will cause the HV to cut out. Not
from wet film, since it happens with film pumped for long periods in the
microscope. Can't observe a rise in pressure when operating shutter so can't
confirm a leak on the shutter shaft O ring.

I would have thought any leak into the camera area would be powerfully
buffered by the pumping capacity up the column and wouldn't affect gun vacuum
enough to cause a cut out.

Any relevant experiences will be gratefully received.

Mel Dickson,
m.dickson-at-unsw.edu.au
The University of NSW,
Sydney, NSW Australia




From: swatkins-at-pop.pitt.edu (Simon Watkins)
Date: Thu, 27 Jul 1995 07:59:51 -0400
Subject: ornithine transcarbamylase

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Message-Id: {199507271159.HAA03612-at-post-ofc01.srv.cis.pitt.edu}
X-Sender: swatkins-at-pop.pitt.edu
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"


Hi folks:

I'm looking for a method to localize ornithine transcarbamylase
HISTOCHEMICALLY, or failing that if anyone knows of a good antibody.... etc

Thanks.
------------------------------------------------------------------
Simon C. Watkins Ph.D
Director; Structural Biology Imaging Center
Scaife 840
University of Pittsburgh
Pittsburgh PA 15261
Tel:412-648-3051
Fax:412-648-1441
-------------------------------------------------------------------





From: JANDT
Date: Wednesday, July 26, 1995 7:58AM
Subject: smooth polymer surface

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Message-Id: {CPLAN218.TREA.735015070095208FCPLAN218-at-ION.CHEVRON.COM}



------------------------------------------------------------------------------


just a thought or two....
Assuming a clear non HIPS sample (crystal PS), the best way to polish the
surface is with a hot, highly polished metal surface. The result would be
similar to injection molding. There is also a kit available from Sporty's
Pilot Shop for polishing acrylic windshields on airplanes. I think it is
called "Micromesh". It might work with polystyrene. It is a slow process
because you must avoid heat build up.


TREA-at-chevron.com
----------

To: MICROSC1--INTERNET MICROSCOPY


} Hi,
}
} I would like to "smoothen" a surface of a polystyrene bulk sample.
}
} The surface roughness should be in the range of nanometer (or better?).
}
} I think normal polishing methods do not apply here since they are
}
} to coarse.
}
} Does anyone have an idea of a good method that could help?
}
} Thanks very much in advance for your help.
}
} jandt-at-msc.cornell.edu
}






From: vickie-at-macc.wisc.edu
Date: Thu, 27 Jul 1995 11:23:03 -0600
Subject: Symposium and Workshop Announcement

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Message-Id: {25072711181059-at-vms2.macc.wisc.edu}

Symposium on:

"Integrated Microscopy"

September 29 to October 1, 1995

Organized by:

Integrated Microscopy Resource (IMR)
a Biomedical Research Resource

at:

The Wisconsin Center
702 Langdon Street
Madison, WI 53706

#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*
Presentations will focus on biological problems for which a combination of
microscopies [i.e. integrated microscopy] has been used. The speakers will
demonstrate by example the power, potential and limitations of various
microscopical techniques. The techniques which will be discussed include:
DIC, Confocal, 2-Photon Excitation Imaging, SEM, TEM, Cryo-specimen
Preparation, and AFM.
#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*

SCHEDULE

FRIDAY (EVENING), SEPTEMBER 29, 1995
6:00 - 7:55 OPENING RECEPTION
8:00 - 8:45 Ken Jacobson, Univ. N. Carolina-Chapel Hill
8:50 - 9:35 John Sedat, Univ. California-San Francisco
9:40 - 10:25 Edward Salmon, Univ. N. Carolina-Chapel Hill

SATURDAY, SEPTEMBER 30, 1995
8:30 - 9:15 D. Lansing Taylor, Carnegie-Mellon University
9:20 - 10:05 Jeff Lichtman, Washington University
10:10 - 10:40 COFFEE BREAK
10:45 - 11:30 John White, Univ. Wisconsin-Madison
11:35 - 12:20 Steven Smith, Stanford University
12:25 - 1:25 LUNCH (on your own)
1:30 - 2:15 Gary Borisy, Univ. Wisconsin-Madison
2:20 - 3:05 Ralph Albrecht, Univ. Wisconsin-Madison
3:10 - 3:55 Paul Bridgeman, Washington University-St. Louis

5:00 - 6:00 EXHIBITOR'S SOCIAL
6:00 - 8:00 BUFFET DINNER

SUNDAY (MORINING), OCTOBER 1, 1995
9:00 - 9:45 Kent McDonald, Univ. California-Berkley
9:50 - 10:35 Stanley Erlandsen, Univ. Minnesota
10:40 - 11:10 COFFEE BREAK
11:15 - 12:00 Hans Ris, Univ. Wisconsin-Madison
12:05 - 12:50 Eric Henderson, Iowa State University


FEES:
General Registration $100.00 (Late Fee: $130.00)
(Includes: Opening Reception, Social and Buffet Dinner, Coffee
Breaks and Materials)

Local Registration $ 80.00 (Late Fee: $110.00)
(Includes: Opening Reception, Social, Coffee Breaks and

Materials)


FOR ADDITIONAL INFORMATION CONSULT OUR WEB SITE

http://www.bocklabs.wisc.edu/imr/imr.html


TO RECEIVE A BROCHURE AND REGISTRATION FORM

WRITE: IMR
Univ. Wisconsin-Madison
1675 Observatory Drive
Madison, WI 53706

OR EMAIL: imradmin-at-calshp.cals.wisc.edu


********************************************************************************

Following the symposium, the IMR will be conducting a 2-day workshop. We
will be presenting lectures and provide "hands-on" experience for the
following techniques:
* 2-photon excitation imaging
* 4D DIC imaging
* Cryo-SEM
* High pressure freezing
* Reversible embeddment for SEM and TEM

Workshop attendence will be limited to 25 participants. A letter of
application is required. Once accepted a fee of $150.00 will be due.

********************************************************************************





From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Fri, 28 Jul 1995 10:29:38 GMT+1200
Subject: Re: JEOL JXA-5A microprobe

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I run an old JEOL JXA-5A probe, which long ago lost its WDS
detectors, and we now use only EDS.

The maximum beam deflection available in the scanning mode is about
200 x 200 microns (-at- 15kV), which is acheived by driving the
deflection coils (X and Y) with about 0.3 volts. Since the coils are
about 6 ohms, this means 50 mA, and 0.015 watts.
I would like to be able to scan a larger area, even if just for the
collection of secondary or backscattered electron images and not for
point analyses.

It seems to me that the reason why JEOL limited the deflection to
such small values was probably so that the beam stayed in the focal
area of the original WD spectrometers. With the EDS setup I feel that
decent analyses could be made at considerably larger deflections,
although this doesn't really matter, my main concern is to be able to
get BSE or SE images of larger areas ie smaller magnifications.
The thing that stops me from just going ahead and building new
deflection coil drivers with more output is my strong desire not
to burn out the deflection coils, as on this 25-year-old instrument
they would not be replaceable, and I would end up with no imaging
facility at all, and a heap of disgruntled users!

I asked my local JEOL branch, in Sydney, Australia, for any
information about the maximum permissible currents through the
deflection coils, but after some time I was informed that such
information was no longer available for an instrument of such age.
I don't really want to pull the column apart to inspect the coils, as
I'm scared of the possible realignment problems on reassembly.

My feeling is that 0.015W is not very much power for what is
presumably a coil wound of copper wire, and that 0.5W could be ok for
any "reasonable" coil. 6 ohms suggests to me either
reasonably thick wire or a very few turns of fine wire. The former
could presumably stand more current (how much? 100mA? 250mA?), the
latter not.

Does anyone out there have any information, experience, or thoughts
which could be useful?

thanks

Ritchie SimsRitchie Sims
Department of Geology
University of Auckland
Auckland, New Zealand




From: em-at-mediacity.com (Ed Monberg)
Date: Thu, 27 Jul 1995 20:29:34 -0800
Subject: Re: JEOL JXA-5A microprobe

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Message-Id: {v01530500ac3e13ea61f0-at-[192.0.2.1]}
Mime-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: quoted-printable

Ritchie,

You need booster amps for these coils. The amps need to have "hard" current=
outputs which put out the appropriate current regardless of the voltage lev=
el.

Collar a friendly EE who knows about Op-Amps, especially ones which can=
dissipate, say, 5 watts and respond at the speeds of interest.

As a target, P=3DE2/R =3D} E=3D(PR)-2, or:

E=3D(6)-2 volts =3D 2.45 volts =3D} 2.45/6=3D=B1400ma=20

Depending on the inductance of the coils and the supplies you choose,
(start with =B115VDC) and a monolithic amp such as NSC OP10,
and a voltage to drive it, it should be fine.



Regards,

Ed Monberg {em-at-mediacity.com}

510-429-1060 Fax 429-1065
LMDC, (Laser Motion Development Co.)
3101 Whipple Road
Union City, CA 94587-1216






From: X.m. Burany :      burany-at-sfu.ca
Date: Fri, 28 Jul 1995 10:16:37 -0700 (PDT)
Subject: Nissei Sangyo Canada, Inc

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Greeting!

Anyone out there can tell me the email address of Mr. Iliya Mekuz at
Nissei Sangyo Canada, Inc. I have a question about making a profile of C.

I greatly appreciate your help.

Sandy Burany

Dept. of Physics
Simon Fraser University
Burnaby B.C. V5A 1S6
Email: xm_burany-at-sfu.ca




From: William Tivol :      tivol-at-wadsworth.org
Date: Fri, 28 Jul 1995 16:49:15 -0500 (EDT)
Subject: Re: Cytochem. Biol.

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Greg Erdos writes:
}
} Is anyone aware of a stain or cytochemical reaction to detect the presence
} of iodine in tissue?
}
Dear Greg,
Starch reacts with iodine, but not I-, and the reaction is very
sensitive. Use something to oxidise the I- to I (if necessary or desir-
able), and add starch solution. I used this method to detect peptides
by perparing chloropeptides, adding KI to get iodopeptides, then adding
starch. The method showed the presence of peptide when ninhydrin showed
nothing. Good luck.
Yours,
Bill Tivol




From: BARBARA.HARTMAN-at-1773.220.SCHERING-PLOUGH.sprint.com
Date: Fri, 28 Jul 1995 13:53:38 -0400
Subject: Antibody Company

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X400-Received: by /PRMD=SCHERING-PLOUGH/ADMD=TELEMAIL/C=US/; Relayed; Fri, 28 Jul 1995 13:53:38 -0400
X400-Received: by /PRMD=SCHERING-PLOUGH/ADMD=TELEMAIL/C=US/; Relayed; Fri, 28 Jul 1995 13:53:38 -0400
X400-Received: by /PRMD=SCHERING-PLOUGH/ADMD=TELEMAIL/C=US/; Relayed; Fri, 28 Jul 1995 13:53:38 -0400



A colleague is interested in the phone number and location of a
company called 'Caltag' that sells antibodies. Any information will be
greatly appreciated.

Thank you!


Barbara Hartman
E-Mail: Barbara.Hartman-at-Schering-Plough.sprint.com
Phone: 201-579-4343
Fax: 201-579-4211






From: Paul Hearn :      paul-at-redland.demon.co.uk
Date: Thu, 27 Jul 1995 20:22:40 GMT
Subject: SEM

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(In reply to your message dated Monday 24, July 1995)

--
---------------------------------------------------------------------------
| Paul Hearn 88 Eastern Avenue
Reading RG1 5SF UK
{} - { Tel: + 44 (0) 1734 665 152
Fax: + 44 (0) 1707 373 255
e-mail paul-at-redland.demon.co.uk
---------------------------------------------------------------------------





From: Paul Hearn :      paul-at-redland.demon.co.uk
Date: Thu, 27 Jul 1995 20:22:40 GMT
Subject: SEM

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(In reply to your message dated Monday 24, July 1995)

--
---------------------------------------------------------------------------
| Paul Hearn 88 Eastern Avenue
Reading RG1 5SF UK
{} - { Tel: + 44 (0) 1734 665 152
Fax: + 44 (0) 1707 373 255
e-mail paul-at-redland.demon.co.uk
---------------------------------------------------------------------------





From: Dr. IGOR LAPSKER :      LAPSKER-at-barley.cteh.ac.il
Date: Sun, 30 Jul 1995 20:37:39 +200
Subject: imaging analysis

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Dear microscopy-netters!
Does anybody of you have any PC sharewares (free programs) for Image
Processing and Morfology Analysis?
I have so much structural images from SEM for analysis...
Please send me programs or any helpful information!
Thanks
Siencerely
Igor lapsker at Lapsker-at-barley.cteh.ac.il




From: richard.easingwood-at-stonebow.otago.ac.nz (Richard Easingwood)
Date: Mon, 31 Jul 1995 09:09:13 +1100
Subject: Starfish testis for TEM

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X-Sender: st004718-at-brandywine.otago.ac.nz
Message-Id: {v01510100ac41afd982ae-at-[139.80.120.161]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

I have been asked to process a range of starfish tissues which include the
external layers and gonad tissue.
I propose to fix the soft tissues in 2% glutaraldehyde made up in filtered
seawater, buffer wash in 0.2m cacodylate buffer plus 2% NaCl (approx 1000
mosmols) then fix in osmium tetroxide in 0.2M buffer.

Question: Do I need to use 0.2M buffer to wash the tissue before OsO4
fixation or can I use the seawater with the OsO4? My concern is whether the
seawater and OsO4 react.
My main problem is as follows: I assume I have to decalcify the external
layers. Can someone please send me a method for decalcifying tissues from
marine specimens?
My standard mammalian decalcifying fluid is as follows: 41.3 disodium EDTA,
4.4g NaOH and make up to 1000mls with distilled water.
To use the fluid I made up as follows: 1.75 % glutaraldehyde in
decalcifying fluid.
Could I make up the decalcifying fluid in seawater instead of distilled
water or will the EDTA react with seawater?

Thanks in anticipation.

Allan Mitchell

Richard Easingwood
South Campus Electron Microscope Unit
Otago Medical School
PO Box 913
Dunedin
NEW ZEALAND

Telephone: 64-03-479 7301
Facsimile: 64-03-479 7254

"The southernmost electron microscope unit in the world"






From: waheeschen-at-dow.com (Bill Heeschen (517)636-4005 PMRC/ASL)
Date: Sun, 30 Jul 1995 17:23:34 -0400
Subject: Re: imaging analysis

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Message-Id: {199507302123.AA02226-at-na3.dow.com}

Igor:
The best software that fits the description of free image analysis software
for manipulation and morphology analysis is NIH Image (from the US National
Institutes of Health). It will run on virtually any Apple Macintosh
computer you have available to you. NIH Image contains a powerful
scripting language and the PASCAL source code is available free of charge
if you are interested in some serious coding. If you are unable to find a
Macintosh, you can buy a Mac emulator for your DOS/Windows computer which
has been successfully tested with the more-recent versions of NIH Image.

NIH Image is available by anonymous FTP at:
zippy.nimh.nih.gov

There is a mail list for discussion of NIH Image (and general digital
imaging issues, as well). To subscribe, send the message:
subscribe nih-image YourNameHere
to the list server:
listproc-at-soils.umn.edu

For a fee of $100 you can get the NIH Image software and manual (I think)
mailed to you from:
NTIS
5285 Port Royal Road
Springfield, VA 22161 USA

The Macintosh emulation software which runs under DOS and Windows 3.1 is
called Executor and is available for $99. Contact them by e-mail at:
questions-at-ardi.com

Good Luck,

Bill
==========================
Bill Heeschen/Analyt. Sci.- Mat. Char. -----
1897-F Building / The Dow Chemical Co. --- ---
Midland, MI 48667 U.S.A. --- D O W ---
phone: (517)636-4005 fax: (517)636-5453 --- ---
Email: waheeschen-at-dow.com -----
========================== R




From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Mon, 31 Jul 1995 16:27:06 GMT+1200
Subject: Re:increased scan on JXA-5A

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Thank you to those who replied to my query, this is a marvellous
facility.

I took up my courage and screwdrivers, gained access to the
deflection coils, and was able to measure the coil wire gauge, which
is, in case anybody ever needs to know, about 0.15mm diameter ie
about the same as 3 Amp fuse wire. So I made a similar test coil on a
plastic former, which softened the former at 0.8 A but seems
indefinitely content at 0.4 A.
This gives me sufficient courage to up the drive to the coils by a
factor of 5X the present max of 50mA ie 250mA.

thanks again

Ritchie SimsRitchie Sims
Department of Geology
University of Auckland
Auckland, New Zealand




From: Dr. IGOR LAPSKER :      LAPSKER-at-barley.cteh.ac.il
Date: Mon, 31 Jul 1995 13:01:34 +200
Subject: image analysis

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Dear microscopy-netters!
Does anybody of you have any PC sharewares (free programs) for Image
Processing and Morfology Analysis?
I have so much structural images from SEM for analysis...
Please send me programs or any helpful information!
Thanks
Siencerely
Igor lapsker at Lapsker-at-barley.cteh.ac.il




From: dgillan-at-ulb.ac.be (Gillan David)
Date: Mon, 31 Jul 1995 12:27:59 +0200 (DST)
Subject: Re: starfish testis for TEM

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Dear Allan Mitchell,

We are working in a marine biology laboratory studying
echinoderms. For electron microscopy, we fix routinely our samples
with the following protocol (all in final concentrations):
- fixation: 3 hours in glutaraldehyde 3%, cacodylate buffer 0.1M,
NaCl 1,55%. pH=7.8, osmolarity: 1030 mOsm.
- buffer washes: 3 x 10 minutes in cacodylate buffer (0.2M), NaCl
1.84%. pH=7.8, 1030 mOsm.
-postfixation: 30' in OsO4 1%, cacodylate buffer 0.1M, NaCl 2.3%.`
pH=7.8, 1030 mOsm.
- three buffer washes before deshydratation.

According to our experience in the subject, this protocol seems to be
one of the best to fix the tegument and other tissues in
echinoderms.

If you need to decalcify your samples, there are two methods:
-simple embedding method: after postfixation and the three buffer
washes, one bath of EDTA 10% (pH 7.8) during 24 hours. This
method is easy but the ultrastructure is poorly preserved (diffuse
appearance).
-double embedding method: here, you decalcify the samples after
the embedding. As the EDTA needs to reach the calcified parts, you
must abrade the resin. It is very important that each skeletal
element (spines, plates...) be at the surface of the abraded resin
otherwise the decalcification will be incomplete. Then one bath of
EDTA 10% pH 7.8 in a vacuum jar during 48 hours, followed by a
wash of bidistilled water (24 hours, under stirring). Dry your
samples on filter paper before desiccation by P2O5 in a vacuum jar
during 24 hours. Then impregnation with the resin (Spurr) in the
vacuum jar during three hours (with P205 inside the jar) before
final embedding.
This method is described in Holland & Grimmer 1981. Cell Tissue
Res. 214: 207-217. Altough longer, this method gives a better
ultrastructure than the first method.


Good luck and fun,

Laurent Ameye & David Gillan
e-mail : lameye-at-ulb.ac.be

Marine Biology Laboratory (CP 160/15)
Free University of Brussels (ULB)
50 Av F.D. Roosevelt
B-1050 Brussels, Belgium.
Fax: ++/32/2/6502796
Tel: ++/32/2/6502970





From: lameye-at-ulb.ac.be (Ameye Laurent)
Date: Mon, 31 Jul 1995 18:59:31 +0200 (DST)
Subject: Re:starfish testis for TEM

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Dear Allan Mitchell,

We are working in a marine biology laboratory studying
echinoderms. For electron microscopy, we fix routinely our samples
with the following protocol (all in final concentrations):
- fixation: 3 hours in glutaraldehyde 3%, cacodylate buffer 0.1M,
NaCl 1,55%. pH=7.8, osmolarity: 1030 mOsm.
- buffer washes: 3 x 10 minutes in cacodylate buffer (0.2M), NaCl
1.84%. pH=7.8, 1030 mOsm.
-postfixation: 30' in OsO4 1%, cacodylate buffer 0.1M, NaCl 2.3%.`
pH=7.8, 1030 mOsm.
- three buffer washes before deshydratation.

According to our experience in the subject, this protocol seems to be
one of the best to fix the tegument and other tissues in
echinoderms.

If you need to decalcify your samples, there are two methods:
-simple embedding method: after postfixation and the three buffer
washes, one bath of EDTA 10% (pH 7.8) during 24 hours. This
method is easy but the ultrastructure is poorly preserved (diffuse
appearance).
-double embedding method: here, you decalcify the samples after
the embedding. As the EDTA needs to reach the calcified parts, you
must abrade the resin. It is very important that each skeletal
element (spines, plates...) be at the surface of the abraded resin
otherwise the decalcification will be incomplete. Then one bath of
EDTA 10% pH 7.8 in a vacuum jar during 48 hours, followed by a
wash of bidistilled water (24 hours, under stirring). Dry your
samples on filter paper before desiccation by P2O5 in a vacuum jar
during 24 hours. Then impregnation with the resin (Spurr) in the
vacuum jar during three hours (with P205 inside the jar) before
final embedding.
This method is described in Holland & Grimmer 1981. Cell Tissue
Res. 214: 207-217. Altough longer, this method gives a better
ultrastructure than the first method.


Good luck and fun,

Laurent Ameye & David Gillan
e-mail : lameye-at-ulb.ac.be

Marine Biology Laboratory (CP 160/15)
Free University of Brussels (ULB)
50 Av F.D. Roosevelt
B-1050 Brussels, Belgium.
Fax: ++/32/2/6502796
Tel: ++/32/2/6502970





From: Daniel Possin :      oemlab-at-u.washington.edu
Date: Mon, 31 Jul 1995 13:01:01 -0700 (PDT)
Subject: ACLAR plastic for TEM embedding

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Hi everyone -

I'm looking for a source of the plastic sheet material made of ACLAR
(TM?). A source of slides and/or coverslips made of the stuff would be
ideal, but I will go for anything that might be used in lieu thereof.
Also, if anyone has experience using this material for TEM embedding, I
would be interested in their technique and opinion. Thanks in advance for
any help you can give me.

Dan

%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
% %
% Daniel Possin Work: 206/ 543-7489 %
% Dept. of Ophthalmology RJ-10 FAX: 206/ 543-4414 %
% University of Washington Home: 206/ 778-1714 %
% Seattle WA 98195 USA Email: oemlab-at-u.washington.edu %
% %
% "The chinese expression 'cheung meng ba sui, gong hey fat choy' is %
% equilvalent to Vulcan expression 'live long and prosper'. It's a %
% small universe and getting smaller everyday". %
% %
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%





From: D5DIH-at-aol.com
Date: Mon, 31 Jul 1995 23:20:44 -0400
Subject: SEM FRAME GRABBER

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CAN ANYONE RECOMMEND AN INEXPENSIVE FRAME GRABBER THAT CAN EASILY INTERFACE
WITH AN ANALOG JEOL 840-I , PC PREFERABLE . I HAVE A 486 WITH 16 MEG RAM
THATS READY FOR IT BUT COULD ALSO GET HOLD OF A MAC. I ALSO HAVE ALL THE
IMAGE ANALYSIS SOFTWARE. MY ONLY PRESENT WAY TO DIGITIZE SEM IS BY SCANNING
MY 4X5 NEGS, BUT WOULD LIKE TO SKIP THE PHOTO STEPS. ATTEMPTING TO QUANTITATE
IMMUNO GOLD SIGNALS IN BSE MODE (SLOW SCAN)




From: Jan Coetzee Elek-mik x2075 NW2 K1-39 :      JANC-at-ccnet.up.ac.za
Date: Tue, 1 Aug 1995 08:30:13 GMT+2
Subject: Re: SEM 840 frame grabber

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} CAN ANYONE RECOMMEND AN INEXPENSIVE FRAME GRABBER THAT CAN EASILY
INTERFACE
} WITH AN ANALOG JEOL 840-I , PC PREFERABLE . I HAVE A 486 WITH 16
MEG RAM
} THATS READY FOR IT BUT COULD ALSO GET HOLD OF A MAC. I ALSO HAVE
ALL THE
} IMAGE ANALYSIS SOFTWARE. MY ONLY PRESENT WAY TO DIGITIZE SEM IS BY
SCANNING
} MY 4X5 NEGS, BUT WOULD LIKE TO SKIP THE PHOTO STEPS. ATTEMPTING TO
QUANTITATE
} IMMUNO GOLD SIGNALS IN BSE MODE (SLOW SCAN)

It is difficult to interface signals from the 840, but a board
that does this successfully is the ImageSlave from MEECO.
This works with a PC and is not too difficult to install if you
take great care to use correctly routed, shielded cable. The board
will grab 840 signals at Slow Scan 1, Slow Scan 2 and Photo Scan. TV-
rate and Ultra-rapid scan modes are not supported.
Contact: Steve Wisbey
10 Seville St
North Parramatta
N.S.W. 2151
Australia
Tel 02-630-7755
Fax 02-630-7365

I do not have any commercial interest in this company, other than as
a customer.

Jan Coetzee
Unit for Electron Microscopy Tel:+27-12-420-2075
University of Pretoria Fax:+27-12-342-1738
Pretoria Internet:janc-at-ccnet.up.ac.za
0002 South Africa




From: STEVEN E SLAP :      75767.640-at-compuserve.com
Date: 01 Aug 95 08:02:06 EDT
Subject: Re: Liquid Nitrogen Level Monitors

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There are two solutions to your problem. You can purchase a gravity feed dewar
with a low-warning switch and automatic fill (10L, 20L, 30L, 50L), or you can
purchase an automatic liquid level control with a solenoid as you remember to
attach to an existing dewar. Please contact me directly by fax (413-789-2786)
or phone (800-992-9037) for pricing and other commercial details.
Steven Slap, Vice-President, Energy Beam Sciences





From: tothal-at-falcon.mufi.hu (Toth Attila)
Date: Tue, 1 Aug 1995 17:45:36 +0200
Subject: Subscription request

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From: USERHHXS
Date: Monday, July 31, 1995 4:16PM
Subject: Liquid Nitrogen Level Monitors

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I'm interested in commercially available liquid
nitrogen level auto filling devices-i.e.some kind
of solenoid valve arrangement that keeps the dewars
filled, automatically. I seem to recall seeing these
on EDS detector dewars, while I was in Berkeley, but
I haven't run across anything amongst the retailers.




From: gwe-at-biotech.ufl.edu (Greg Erdos)
Date: Tue, 1 Aug 1995 09:16:43 GMT
Subject: Re: ACLAR plastic for TEM embedding

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} Hi everyone -
}
} I'm looking for a source of the plastic sheet material made of ACLAR
} (TM?). A source of slides and/or coverslips made of the stuff would be
} ideal, but I will go for anything that might be used in lieu thereof.
} Also, if anyone has experience using this material for TEM embedding, I
} would be interested in their technique and opinion. Thanks in advance for
} any help you can give me.
}
} Dan
}

} %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
} % %
} % Daniel Possin Work: 206/ 543-7489 %
} % Dept. of Ophthalmology RJ-10 FAX: 206/ 543-4414 %
} % University of Washington Home: 206/ 778-1714 %
} % Seattle WA 98195 USA Email: oemlab-at-u.washington.edu %
} % %
} % "The chinese expression 'cheung meng ba sui, gong hey fat choy' is %
} % equilvalent to Vulcan expression 'live long and prosper'. It's a %
} % small universe and getting smaller everyday". %
} % %
} %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
}
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Dear Dan,

Aclar plastic is available from ProPlastics, Inc., P.O. Box 1489, Linden
N.J. 07036

Phone: 908-925-5555. This info is now 3 years old, which is when I made the
minimum purchase which will last several lifetimes,/. I am willing to send
a small sample to anyone who would like to try it before they buy.

The material is optically clear and will not bind to any embedding resin we
have tried. Many cell lines will grow on it. It is flexible so it tends to
curve if steps are not taken to prevent that. I have taken to "spot
welding" it with a hot dissecting needle to a piece of an old EM negative or
to the bottom of a culture dish.}

Let me know if you would like some to try.
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
-at- Greg Erdos Phone: 904-392-1295
-at-
-at- Scientific Director,
-at-
-at- ICBR Electron Microscopy Core Lab
-at-
-at- 218 Carr Hall Fax 904-846-0251
-at-
-at- University of Florida E-mail: gwe-at-biotech.ufl.edu
-at-
-at- Gainesville, FL 32611
-at-
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-





From: Patty Jansma :      plj-at-manduca.neurobio.arizona.edu
Date: Tue, 01 Aug 1995 11:04:30 -0700 (MST)
Subject: Re: ACLAR plastic for TEM embedding

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Ted Pella carries ACLAR sheets.

cat. # 10502
Ted Pella
P.O. Box 49477
Redding, CA 96049

Patty Jansma Tel:602-621-6671
plj-at-manduca.neurobio.arizona.edu
Arizona Research Labs Division of Neurobiology
University of Arizona


On Mon, 31 Jul 1995, Daniel Possin wrote:

} Hi everyone -
}
} I'm looking for a source of the plastic sheet material made of ACLAR
} (TM?). A source of slides and/or coverslips made of the stuff would be
} ideal, but I will go for anything that might be used in lieu thereof.
} Also, if anyone has experience using this material for TEM embedding, I
} would be interested in their technique and opinion. Thanks in advance for
} any help you can give me.
}
} Dan
}
} %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
} % %
} % Daniel Possin Work: 206/ 543-7489 %
} % Dept. of Ophthalmology RJ-10 FAX: 206/ 543-4414 %
} % University of Washington Home: 206/ 778-1714 %
} % Seattle WA 98195 USA Email: oemlab-at-u.washington.edu %
} % %
} % "The chinese expression 'cheung meng ba sui, gong hey fat choy' is %
} % equilvalent to Vulcan expression 'live long and prosper'. It's a %
} % small universe and getting smaller everyday". %
} % %
} %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
}







From: Strucural Biology Unit :      MICROSCOPY-at-SBSNOV1.AUCKLAND.AC.NZ
Date: Wed, 2 Aug 1995 08:49:48 GMT+1200
Subject: Membrane dye for brightfield.

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Dear microscopists,

I have some live transparent tissue and would like to see the
membranes in bright field. For practicle reasons I can't use
phase contrast,DIC etc. to see them. Can anyone suggest a dye/stain
that won't immediately kill the cells. Ideally the cells should
remain active for an hour or two after dye application and I'm not
worried about longer term cell viability.

Again, for practicle reasons the dye sould NOT be fluorescently based
as I have tried several of these without success due to practicle
considerations.

TerryMicroscopy Unit
School Of Biological Sciences
The University Of Auckland
Level 1, Thomas Building
Private Bag 92019
Auckland, NEW ZEALAND

phone:(09) 373 7999 ext 5986
fax: (09) 373 7417




From: Keith Moulding :      MCMOULDK-at-usthk.ust.hk
Date: 02 Aug 1995 10:01:08 +0800
Subject: Re: SEM frame grabber

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You could try the ImageSlave board. It captures the images at 1K
x 1K when you hit the photo button. Images are saved as TIFF
files and it has the ability to increment the file number series
so you do not have to type in a filename each time. Currently
runs under DOS, but a Windows version will be very soon.

Your PC sounds fine for it. We have two of the boards here and
are very happy with them, they have saved a lot of Polaroid film.

It depends where you are, but there is an advert in Microscopy
and Analysis for the board. Otherwise contact Steve Wisbey, Meeco
Holdings PTY. Ltd, FAX +61 (2) 6307365 (Australia).


Keith.


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr. Keith Moulding, ~
Materials Characterisation and Preparation Centre, ~
Hong Kong University of Science and Technology, ~
Clear Water Bay, ~
Kowloon, ~
Hong Kong. ~
~
Tel: (852) 2358 8724 ~
Fax: (852) 2358 2451 ~
~
E-mail: mcmouldk-at-usthk.ust.hk ~
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~




From: Kukin V.N. :      lemi-at-mx.iki.rssi.ru
Date: Wed, 2 Aug 1995 12:30:54 -0300
Subject: ICXOM E-mail?

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Dear All,
Does anyone know the E-mail of the secretariat of the ICXOM -
The 14th International Congress on X-ray Optics and Microanalysis?
ICXOM will be held on Aug.29 - Sept.2, 1995 in the Guangzhou, China.
I very need the fast communication with the Organizing Commitee
and the post is too slow.
Many thanks!

Sergey Kramar
CFPM, Moscow Institute of Electronic Technology,
Moscow 103498, Russia
E-mail: lemi-at-mx.iki.rssi.ru (to S.Kramar)




From: goran.alsterborg-at-mailbox.swipnet.se (JEOL Service)
Date: Wed, 02 Aug 1995 12:57:44 +0000
Subject: SEM FRAME GRABBER

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Message-Id: {199508021103.NAA20135-at-mailbox.swip.net}
X-Sender: m-13225-at-mailbox.swip.net (Unverified)
Mime-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: quoted-printable


} CAN ANYONE RECOMMEND AN INEXPENSIVE FRAME GRABBER THAT CAN EASILY INTERFACE
} WITH AN ANALOG JEOL 840-I , PC PREFERABLE . I HAVE A 486 WITH 16 MEG RAM
} THATS READY FOR IT BUT COULD ALSO GET HOLD OF A MAC. I ALSO HAVE ALL THE
} IMAGE ANALYSIS SOFTWARE.

One commersially available solution could be the JEOL SemAfore, which is a=
=20
passive listening digitizer board for IBM compatible PC operating under MS=
=20
Windows. This will digitize the slow scan signal from a JEOL JSM-840 (and=20
most other JEOL SEMs) with the same pixel resolution (typically 1900x1400=20
pixels) as displayed on your photo CRT.

The digitized image is stored in standard BMP format, which is read by most=
=20
other MS Windows applications. If you still wish to record the original or=
=20
modified image photographically, you can send the image back to the photo=20
CRT of your SEM.

The minimum PC configuration needed is a 486SX with 8 MB RAM and MS-Windows=
3.1.

As I don=B4t know where you are located, please contact me directly for=20
commercial details.

Best regards

Goeran Alsterborg
JEOL(Skandinaviska)AB Phone: +46-8-28 28 00
Vegagatan 19 Fax: +46-8-29 16 47
172 34 Sundbyberg =20
Temporary e-mail:=
goran.alsterborg-at-mailbox.swipnet.se
SWEDEN






From: Richard E. Edelmann :      EDELMARE-at-CASMAIL.MUOHIO.EDU
Date: Wed, 2 Aug 1995 09:37:18 -500
Subject: Vacuum Technology Book.

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Message-Id: {9508021100.AA33290-at-pukrs7.puk.ac.za}

In a recent wirlwind clearing of stored E-mail messages I have lost
the information regarding a Vacuum Technology Book which has been
mentioned on the list by the author a number of times. (Most
recently regarding my questions on replacing Silicone Diff pump oil).

I am looking for the Title , Author, Publisher, etc. of this book if
anyone could provide this info I'd greatly appreciate it.

Thank you for your tolerance of my stupidity....


Humbly yours...




From: ingen-at-Rt66.com
Date: Wed, 02 Aug 1995 07:47:20 -0600
Subject:

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Message-Id: {9508021344.AA16943-at-Rt66.com}
X-Sender: ingen-at-rt66.com
X-Mailer: Windows Eudora Version 1.4.4
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

subscribe microscopy





From: Richard E. Edelmann :      EDELMARE-at-CASMAIL.MUOHIO.EDU
Date: Wed, 2 Aug 1995 10:59:55 -500
Subject: RE:vacuum technology book

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Thank you to all who replied so quickly. I now have the info.





From: Carl A. Palmer :      palmerca-at-skywalkr.nmg.sms.siemens.com
Date: Wed, 2 Aug 1995 10:42:23 -0500 (CDT)
Subject: Mollifex source

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Message-Id: {25080210381596-at-vms2.macc.wisc.edu}



unsubscribe microscopy







From: Michael OKeefe :      Michael_OKeefe-at-macmail4.lbl.gov
Date: 2 Aug 1995 09:43:45 -0700
Subject: Re: LN2 Level monitor -- Wa

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Message-ID: {n1404765624.67193-at-macmail4.lbl.gov}
"Smuts, L" {PLBLS%puknet.puk.ac.za-at-Csa2.LBL.Gov}
X-Mailer: Mail*Link SMTP/QM 3.0.0

Reply to: RE} } LN2 Level monitor -- Warning!

} Date: 8/2/95 4:14 AM
} From: Smuts, L
} } Dear USERHHXS and identifiable microscopists:
} } Many years ago, (20+) we had such a device on a PGT detector. It was
} } nothing but trouble!!!!! The internal pressure in a 160L LN2 tank
} } is....

} Can the cooling down of the PGT EDS crystal also be done with
} liquid air (LAir) instead of Liquid Nitrogen (LN2)?
} Boilingpoint: LN2=-195.8 C ; LAir=?differs with a few deg C from LN2.
} Specific heat: LN2=0.2438 ; LAir=0.2374. (constant pressure)

} \\\\\\\\\\\\\\\\\\\\\} WWW:
} http://www.puk.ac.za} \\\\\\\\\\\\\\\\\\\\\\}
} Leon Smuts-Electronmicroprobe-University of Potchefstroom-South Africa}
} ///////////////////} e-MAIL:
plbls-at-puknet.puk.ac.za} ///////////////////}

-------------------------------------------------------------
Hazard Warning ---
--------------
The problem with an auto-fill system using liquid air is the
fractional distillation effect that gradually enriches the oxygen content
of the remaining cryogenic liquid as the liquid nitrogen evaporates
preferentially. After a few weeks the oxygen-enriched liquid can be
hazardously explosive! (I seem to remember a game that involved
soaking cotton-wool in the liquid and igniting it with a Tesla coil . . . .).
-------------------------------------------------------------
Michael A. O'Keefe
Acting Head NCEM
maok-at-lbl.gov








From: Daniel Possin :      oemlab-at-u.washington.edu
Date: Mon, 31 Jul 1995 13:01:01 -0700 (PDT)
Subject: ACLAR plastic for TEM embedding

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Message-Id: {1995Aug02.100350.1889455155-at-ms.sjdccd.cc.ca.us}
To: oemlab-at-u.washington.edu (Daniel Possin),
microscopy-at-aaem.amc.anl.gov (MSA list)

Ted Pella INC. Sells it.
PO Box 2318
Redding, CA 96099
800/237-3526

Judy M.

Judy Murphy, PhD
Dept. of Microscopy
San Joaquin Delta College
5151 Pacific Ave
Stockton, CA 95207

Phone: 209/474-5284
FAX: 209/474-5649
e-mail:
murphy.ms.sjdccd.cc.ca.us

_______________________________________________________________________________

X-Sender: oemlab-at-saul3.u.washington.edu

Hi everyone -

I'm looking for a source of the plastic sheet material made of ACLAR
(TM?). A source of slides and/or coverslips made of the stuff would be
ideal, but I will go for anything that might be used in lieu thereof.
Also, if anyone has experience using this material for TEM embedding, I
would be interested in their technique and opinion. Thanks in advance for
any help you can give me.

Dan

%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
% %
% Daniel Possin Work: 206/ 543-7489 %
% Dept. of Ophthalmology RJ-10 FAX: 206/ 543-4414 %
% University of Washington Home: 206/ 778-1714 %
% Seattle WA 98195 USA Email: oemlab-at-u.washington.edu %
% %
% "The chinese expression 'cheung meng ba sui, gong hey fat choy' is %
% equilvalent to Vulcan expression 'live long and prosper'. It's a %
% small universe and getting smaller everyday". %
% %
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%



______________________________________________________________________________
San Joaquin Delta College World Wide Web:http://www.sjdccd.cc.ca.us
5151 Pacific Avenue email:webmaster-at-ms.sjdccd.cc.ca.us
Stockton, CA 95207
general information:(209) 474-5151 or FAX-at-(209)474-5600





From: m.dickson-at-unsw.edu.au (melvyn dickson)
Date: Thu, 3 Aug 1995 11:19:44
Subject: Re: Downloading Images from SEM 840

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X-Nupop-Charset: English

We have fitted ImageSlave digitising boards to 4 of our microscopes including
a JEOL SEM 840. They fit in a PC 8 bit slot. The software is very friendly.
We have it fixed so every time the PHOTO button is pressed an image is
digitised concurrently with (or instead of) exposing a film or Polaroid image.
The system then prompts for a filename and saves on the local net to our
server. Will do automatic increments of file numbers. Currently runs under
DOS but a windows version with TWAIN compliance is promised soon. Resolution
currently 1024x1024x12 at Australian Dollars $3500 but 2048x2048x12 is
promised soon

Distributors are world wide. I have a list if it is of wider interest.

ImageSlave distributors for the USA as of 1-Aug-95:

Contact Jim Hilton
Advanced Database Systems
7931 S. Broadway #322
Littleton CO 80122
U.S.A.
Tel: + 1 303 761-5635
Fax: + 1 303 761-592

We are not commercially involved, just happy customers.
Mel Dickson.




From: mark-at-sparky.sets.hawaii.com (Mark Voelker)
Date: Wed, 2 Aug 1995 17:02:37 -1000
Subject: TEM/SEM: Old microscopes for donation?

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Message-Id: {9508030253.AA10815-at-sparky.sets.hawaii.com}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

The Alcor Foundation, a 501c(3) nonprofit organization located in Scottsdale,
Arizona, needs to acquire a working TEM and/or SEM for use in cryobiological
research. Are there any old instruments that someone would be willing to
donate to us? Such a donation would be tax deductible.
Thank You,
Mark A. Voelker, PhD
Alcor Foundation
7895 E. Acoma Drive
Scottsdale, AZ 85260
telephone (602)922-9013

Mark Voekler
SETS Technology Inc.
300 Kahelu Ave.,Suite 10
Mililani, HI 96789
Vox: 808-625-5262
Fax: 808-625-2474






From: richard.easingwood-at-stonebow.otago.ac.nz (Richard Easingwood)
Date: Thu, 3 Aug 1995 17:00:54 +1100
Subject: TEM technique for human cilia?

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Does anyone have a reliable technique for preparing human cilia, in
particular dynein arms, for transmission electron microscopy? This is in
cases of query immotile cilia syndrome.

Thanks in advance,
Zyg Poczwa

Please reply to: richard.easingwood-at-stonebow.otago.ac.nz

South Campus Electron Microscope Unit
Otago Medical School
PO Box 913
Dunedin
NEW ZEALAND

Telephone: 64-03-479 7301
Facsimile: 64-03-479 7254






From: richard.easingwood-at-stonebow.otago.ac.nz (Richard Easingwood)
Date: Thu, 3 Aug 1995 08:59:53 +0100 (BST)
Subject: re:Liquid Nitrogen Level monit (fwd)

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Message-Id: {199508030759.IAA22419-at-zeus.bris.ac.uk}

} liquid air (LAir) instead of Liquid Nitrogen (LN2)?
} Boilingpoint: LN2=-195.8 C ; LAir=?differs with a few deg C from LN2.
} Specific heat: LN2=0.2438 ; LAir=0.2374. (constant pressure)
}
} } Leon Smuts-Electronmicroprobe-University of Potchefstroom-South Africa}
} } ///////////////////} e-MAIL: plbls-at-puknet.puk.ac.za} ///////////////////}
}
}
I've never sat down and thought about this in detail to see if it's true,
but I was told once upon a time, when the liquid nitrogen machine was down
and we had to use liquid air, that it was fine for a short while, but that
if you kept on using liquid air, you could accumulate liquid oxygen in your
dewar - not desirable!

--
Keith R. Hallam | Owner of two cats, an '86 MR2, two melodeons,
Research Associate | member of Rag Morris and Bristol Fashion and
| a surface analyst, depending on the discussion
University of Bristol, | group
Interface Analysis Centre, | Telephone: National (0117) 925 5666
Oldbury House, | International + 44 117 925 5666
121, St. Michael's Hill, | Facsimile: National (0117) 925 5646
Bristol, | International + 44 117 925 5646
BS2 8BS, | E-mail: k.r.hallam-at-bristol.ac.uk
England | URL: http://zeus.bris.ac.uk/~phkrh/




From: lmiller-at-ux1.cso.uiuc.edu (Lou Ann Miller)
Date: Thu, 3 Aug 1995 07:46:28 -0600
Subject: Re: TEM technique for human cilia?

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Message-Id: {n1404643651.77911-at-qm-japan.kla.com}

Hi!

I've done this in dogs, humans etc once in a while.

I usually split the sample:

a. Std TEM, using Uranyl Acetate en block staining--saturated solution
(aq) for 30-60 minutes

b. Std TEM with Tannic acid enblock staining.
-----------------------------------------------------------------------

Microscopic Imaging Laboratory Embedding

Tannic Acid Fix

Fixation and embedding Procedure for Ciliated Samples to Visualize
Protofilaments and Dynein Arms.

Special reagents needed:


0.1 M PBS, 0.5% Triton X-100 buffer:

1. Dilute stock(10%) TritonX-100 1/10 with water
to make a final solution of 1% Triton X-100

2. Use one part 0.2M PBS buffer added to one part of
1% Triton X to make the final solution.


4% Tannic Acid in Buffer:

0.4 grams of EM grade Tannic Acid

10 mls of 0.1M Triton X buffer
___________________________________
*** Make fresh with every use.



Embedding:

1. After fixing in EM fix or Karnovsky's, rinse with buffer.

2. Incubate tissue in the 0.5% triton X /tannic acid solution for
1 hour, rotating at room temperature.

3. Remove solution, rinse with buffer.

4. Incubate in EM fix or Karnovsky's for 1 hour.

5. Rinse in buffer.

6. Incubate 2 hours in OsO4, Omit any usage of KCN or UA.

7. Continue as in standard embedding with dehydrations and
infiltrations.

Reference:

Silsman, N.J. / C.E. Famum and D.K. Reed
Variability of ciliary ultrastructure in normal dogs.
----------------------------------------------------------------------

It also sometimes helps to have a tilt mechanism on the TEM to bring cross
sections of cilia into better focus alignment.

Hope this helps, use EM Grade Tannic Acid and make it fresh.

Lou Ann




} Does anyone have a reliable technique for preparing human cilia, in
} particular dynein arms, for transmission electron microscopy? This is in
} cases of query immotile cilia syndrome.
}
} Thanks in advance,
} Zyg Poczwa
}
} Please reply to: richard.easingwood-at-stonebow.otago.ac.nz
}
} South Campus Electron Microscope Unit
} Otago Medical School
} PO Box 913
} Dunedin
} NEW ZEALAND
}
} Telephone: 64-03-479 7301
} Facsimile: 64-03-479 7254

***********************
Lou Ann Miller
Microscopic Imaging Laboratory
College of Veterinary Medicine
University of Illinois
2001 S Lincoln Ave
Urbana, Illinois 61801
217-244-1566
lmiller-at-ux1.cso.uiuc.edu

Microscopy Lab:
http://www.cvm.uiuc.edu/announcements/MicSoc/MicImagLab.html

Personal Home Page:
http://www.cvm.uiuc.edu/HomePages/LouAnnMiller/Homepage.html
***********************






From: gwe-at-biotech.ufl.edu (Greg Erdos)
Date: Thu, 03 Aug 1995 10:47:55 -0500 (EST)
Subject: Fee Structures

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Several times ther have been requests for information of fee structures of
university based service laboratories,, doing EM . Has this information
been summarized and archived somewhere where I might get to it? I do not
want to request information that might already be available.

Thanks in advance, Greg
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
-at- Greg Erdos Phone: 904-392-1295
-at-
-at- Scientific Director,
-at-
-at- ICBR Electron Microscopy Core Lab
-at-
-at- 218 Carr Hall Fax 904-846-0251
-at-
-at- University of Florida E-mail: gwe-at-biotech.ufl.edu
-at-
-at- Gainesville, FL 32611
-at-
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-





From: smithj-at-acad.winthrop.edu
Date: Thu, 3 Aug 1995 11:06:25 -0400
Subject: ?Printers: Epson Stylus users

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Microscopists:
We are shopping for a color/monochrome printer to hook to our in-house
microscopy network, which is Appletalk. The printer would be used
for color output for poster presentations and for monochrome output
from the Mac hooked to our scanning EM. I hear the color output
from the stylus is spectacular. How good is the *monochrome*
output?

TIA
Julian Smith III
Biology
Winthrop University
Rock Hill SC 29733
smithj-at-winthrop.edu




From: KJMcCarthy :      KJMCCARTHY-at-bmg.bhs.uab.edu
Date: Thu, 3 Aug 1995 10:15:59 CST+6CDT
Subject: Mollusk mitochondria fixation-help!!!!!

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Message-ID: {MAILQUEUE-101.950803101144.544-at-bmg.bhs.uab.edu}

Colleagues,
I have been approached by a colleague whose research focuses on the physiology of a species of clam
that lives in high-sulfide environments. She is investigating the function of the mitochondria in these
particular organisms and wants to do some correlative morphology work. I am willing to help her out
but have never in my life had to deal with a mollusk. Is there anything peculiar about fixation, tissue
processing ect. that I should be aware of before undertaking this project?

Thanks in advance,

Kevin
Kevin McCarthy
Assistant Professor
Department of Cell Biology
Digital Imaging Microscopy Facility
University of Alabama at Birmingham
Birmingham, Alabama 35294
Phone 205-934-9923/9924
Fax 205-934-7029
"Seeing the World Through Different Eyes"




From: Marc Brande :      brande-at-SDSC.EDU
Date: Thu, 3 Aug 1995 08:42:03 -0700 (PDT)
Subject: Cell monolayer transport

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Neuroscience List {neur-sci-at-net.bio.net} ,
Cell Bio List {cellbiol-at-net.bio.net}
Message-Id: {Pine.3.05.1.9508030803.A2104-9100000-at-pauline.sdsc.edu}
Mime-Version: 1.0
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Please excuse if slightly off topic:

What are the best culture vessels for sending live cell cultures through
the mail to avoid cell damage? Thanks so much.

Marc

Marc C. Brande, M.S. SD3D Email List:3D Imaging
San Diego 3D Imaging Group To subscribe/unsubscribe:listserv-at-bobcat.etsu.edu
3840 Camino Lindo To post a message:sd3d-at-bobcat.etsu.edu
San Diego, CA 92122 Email:BRANDE-at-SDSC.EDU Voice:619-587-4830





From: smithj-at-acad.winthrop.edu
Date: Thu, 3 Aug 1995 11:06:25 -0400
Subject: ?Printers: Epson Stylus users

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Message-Id: {1995Aug03.093442.1158838932-at-ms.sjdccd.cc.ca.us}
To: microscopy-at-aaem.amc.anl.gov (MSA list), smithj-at-acad.winthrop.edu (smithj)

Monochrome output is also very good. I use it for both SEMs and TEMs.Black
are a true black. Cartridges are separate i.e. CYMK and BW. You can order
special glossy paper which should give it a photographic look. The
resolution is already photographic but with glossy paper it should be
super. Regular glossy paper does not work as I have tried it.
Good luck, Judy M
PS At the price you may want to buy two. They are slow for 720 depi
printing but well worth the wait.

Judy Murphy, PhD
Dept. of Microscopy
San Joaquin Delta College
5151 Pacific Ave
Stockton, CA 95207

Phone: 209/474-5284
FAX: 209/474-5649
e-mail:
murphy.ms.sjdccd.cc.ca.us

_______________________________________________________________________________


Microscopists:
We are shopping for a color/monochrome printer to hook to our in-house
microscopy network, which is Appletalk. The printer would be used
for color output for poster presentations and for monochrome output
from the Mac hooked to our scanning EM. I hear the color output
from the stylus is spectacular. How good is the *monochrome*
output?

TIA
Julian Smith III
Biology
Winthrop University
Rock Hill SC 29733
smithj-at-winthrop.edu


______________________________________________________________________________
San Joaquin Delta College World Wide Web:http://www.sjdccd.cc.ca.us
5151 Pacific Avenue email:webmaster-at-ms.sjdccd.cc.ca.us
Stockton, CA 95207
general information:(209) 474-5151 or FAX-at-(209)474-5600





From: Mr James Wesley-Smith :      WESLEYSM-at-biology.und.ac.za
Date: Thu, 3 Aug 1995 13:39:48 +0200 (SAST)
Subject: Merits of cacodylate buffer?

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Message-ID: {MAILQUEUE-101.950803133948.836-at-biology.und.ac.za}

Dear colleagues
I would like to get an idea of what the general use of sodium
cacodylate is like "out there". My concern is that its arsenic
content makes it an environmentally-unfriendly chemical. I
discourage our users in favour of phosphate or "Good" buffers
(PIPES, etc), and I am unaware of any shortcoming in ultrastructural
preservation as a consequence of this. Furthermore, it is not a
cheap buffer either.

In my opinion, the use cacodylate-based fixatives is justified for
fixing specimens during field- trips, or whenever a buffer is to be
kept at room temperature for a few days/ weeks since it will not
become contaminated with bugs. However, I do have a problem with its
wholesale use as a routine buffer.

What are your thoughts on the subject?

James Wesley-Smith
Electron Microscope Unit
George Campbell Building
University of Natal
Durban, South Africa





From: Michael Rock :      merock-at-u.washington.edu
Date: Thu, 3 Aug 1995 10:13:24 -0700 (PDT)
Subject: Re: ?Printers: Epson Stylus users

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Julian-
We are using the Stylus printer primarily for color images, but after
reading your request I fired up the monochrome mode and printed a TEM
image, the image is nice and for the price (1/10 or 1/20 of a dye sub
print) it is fine. but if time is also included in the criteria, try out
the 600 dpi laser printers HP-4 and/or Apple LaserWriter 16/600, the
images are superior, and the machines are way faster.
-Mike

On Thu, 3 Aug 1995 smithj-at-acad.winthrop.edu wrote:

} Microscopists:
} We are shopping for a color/monochrome printer to hook to our in-house
} microscopy network, which is Appletalk. The printer would be used
} for color output for poster presentations and for monochrome output
} from the Mac hooked to our scanning EM. I hear the color output
} from the stylus is spectacular. How good is the *monochrome*
} output?
}
} TIA
} Julian Smith III
} Biology
} Winthrop University
} Rock Hill SC 29733
} smithj-at-winthrop.edu
}





From: A. Kent Christensen :      akc-at-umich.edu
Date: Thu, 3 Aug 1995 13:47:57 -0400 (EDT)
Subject: Re: Merits of cacodylate buffer?

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I have used cacodylate buffer in the past mostly for the convenience
of being able to make up a 0.2M cacodylate stock and store it long-term in
the refrigerator, knowing it will be there ready for use. In contrast, in
my experience, the usual 0.2M phosphate stocks at refrigerator temperature
tend to crystallize out over time, so when you are ready to make up a
fixative you may face the annoying task of warming the stock and agitating
to get the crystals back into solution. Phosphate stocks are also more
vulnerable to infections by bacteria or fungi, and so you may be greeted
by a thriving colony when you are ready to make up your fixative.

However, as safety requirements become more and more stringent, the
convenience of cacodylate may become counterbalanced by problems of
disposal.

A. Kent Christensen, University of Michigan, {akc-at-umich.edu}

----------------------------------

On Thu, 3 Aug 1995, Mr James Wesley-Smith wrote:

} Dear colleagues
} I would like to get an idea of what the general use of sodium
} cacodylate is like "out there". My concern is that its arsenic
} content makes it an environmentally-unfriendly chemical. I
} discourage our users in favour of phosphate or "Good" buffers
} (PIPES, etc), and I am unaware of any shortcoming in ultrastructural
} preservation as a consequence of this. Furthermore, it is not a
} cheap buffer either.
}
} In my opinion, the use cacodylate-based fixatives is justified for
} fixing specimens during field- trips, or whenever a buffer is to be
} kept at room temperature for a few days/ weeks since it will not
} become contaminated with bugs. However, I do have a problem with its
} wholesale use as a routine buffer.
}
} What are your thoughts on the subject?
}
} James Wesley-Smith
} Electron Microscope Unit
} George Campbell Building
} University of Natal
} Durban, South Africa
}
}




From: AUGOOD :      sarah.augood-at-bbsrc.ac.uk
Date: Fri, 4 Aug 1995 04:49:28 -0500
Subject: Microscope slide boxes ?

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Comments: Converted from PROFS to RFC822 format by PUMP V2.2X

Can anyone help me with a query ?
I am trying to find a supplier of plastic microscope slide boxes
that are light tight and large enough to store glass slides
76 mm x 39 mm. I need to store slides at -70C.
I would be grateful for any help ?
Many thanx.

Sarah Augood
EMAIL:augood-at-bbsrc.ac.uk
FAX:44-1223-836614




From: Microbill-at-aol.com
Date: Fri, 4 Aug 1995 05:57:51 -0400
Subject: Re: ?Printers: Epson Stylus u...

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Another solution to the B&W delima is the new Lexmark Optra R which is a
laser printer that prints at 1200dpi - it's fast and simple -just plug it
in. They cost about $2500 with the memory necessary to print large 1200 dpi
images.

Bill Miller
ElectroImage




From: FRANCISCO J HERNANDEZ BLAZQUEZ fzea - zab 0195 616122 - 283 :      fjhblazq-at-usp.br
Date: Fri, 4 Aug 1995 08:11:19 -0500 (CDT)
Subject: size of ferritin particles

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I'm workin with ferritin from horse spleen (Sigma I) as an
electron microscopic marker. Although it is easy to identify the
particles in the micrographs, I would like to know the exact
size of theses particles. Could you help me, please
Thank you
=============================================================================
Francisco Javier Hernandez Blazquez |
Universidade de Sao Paulo - Faculdade de | e-mail fjhblazq-at-usp.br
Zootecnia e Engenharia de Alimentos | Voice: 55 195 616122 r. 265
Departamento de Ciencias Basicas/Histologia| r. 278
Av. Duque de Caxias Norte, 225 CP 23 | Fax: 55 195 611689
CEP 13630-000 Pirassununga (Sao Paulo) | 55 11 8694831
BRAZIL |
==============================================================================







From: W.L. Steffens :      STEFFENS.B-at-calc.vet.uga.edu
Date: Fri, 4 Aug 1995 09:06:21 EST
Subject: Mollusc fixation

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To Kevin McCarthy:

You didn't say whether they were marine, freshwater, or terrestrial
molluscs...it makes a BIG difference. Aquatic molluscs are
osmoconformers, maintaining a tonicity roughly equivalent to their
environment. My experience with any marine invertebrate invariably finds
it best to make up a fixative (usually GTA-Formaldehyde) in seawater.
Seawater is actually a fairly good buffer.
Freshwater molluscs are more of a problem. Their blood measures only
about 30 mOsmol. The buffers that we use for EM are ineffective at that
concentration. My best results have been to make up 2% GTA-2%Form. in
pondwater. Since its not buffered, the pH should be monitored during
fixation and adjusted appropriately. It tends to progressively lower
during the fixation process, and it should be kept above 7.2. The reason
is that some freshwater molluscs, particularly bivalves contain huge
numbers of calcium phosphate spherulites, which are rapidly lost under
acidic conditions, leaving large empty spaces in the cytoplasm.

For a reference, see:

Steffens, et al. 1985. Can. J. Zool. (63): 348-353.

Good luck!

-=W.L. Steffens=-
Department of Veterinary Pathology
College of Veterinary Medicine
University of Georgia




From: SveEn-at-pai.liu.se (Sverker Enestrom)
Date: Fri, 4 Aug 1995 14:52:05 +0200
Subject: Re: size of ferritin particles

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} I'm workin with ferritin from horse spleen (Sigma I) as an
} electron microscopic marker. Although it is easy to identify the
} particles in the micrographs, I would like to know the exact
} size of theses particles. Could you help me, please
} Thank you
} =============================================================================
} Francisco Javier Hernandez Blazquez |
} Universidade de Sao Paulo - Faculdade de | e-mail fjhblazq-at-usp.br
} Zootecnia e Engenharia de Alimentos | Voice: 55 195 616122 r. 265
} Departamento de Ciencias Basicas/Histologia| r. 278
} Av. Duque de Caxias Norte, 225 CP 23 | Fax: 55 195 611689
} CEP 13630-000 Pirassununga (Sao Paulo) | 55 11 8694831
} BRAZIL |
} ==============================================================================

Ferritin was introduced by Singer & Schick 1961 as a marker. It is a spherical
molecule, 12 nm in outer diameter and a molecular weight of 750,000. It has
an iron
content of 23% by weight. The iron is concentrated in micelles in the center of
the molecule forming a tetrad with a diameter of 5.5-6.0 nm which is the
electron
dense core.
I hope this is what you need.
Sverker



*********************************************************
Sverker Enestrom M.D., Ph.D.
Department of Pathology
University of Linkoping, Sweden
Phone: +46 13 22 15 20
Fax: +46 13 13 22 57
*********************************************************






From: W.L. Steffens :      STEFFENS.B-at-calc.vet.uga.edu
Date: Fri, 4 Aug 1995 09:23:46 EST
Subject: Cacodylate buffer

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Despite its toxicity, cacodylate buffer is not always resistant to
microbial growth. Several years ago, we had something get into our large
volume of cacodylate stock. It gave off a strong garlic smell, and was
teeming with bacteria. One of our microbiologists found it to be a
"demethylating bacterium". Cacodylate, being dimethyarsenic acid is
actually food to some microorganisms. Thereafter, I began "poisoning" my
stocks with sodium azide. So, in reality, resistance to microbial growth
is not a factor in considering its use.

-=W.L. Steffens=-
Department of Veterinary Pathology
College of Veterinary Medicine
University of Georgia




From: Jay Jerome :      jjerome-at-isnet.is.wfu.edu
Date: Fri, 4 Aug 1995 11:05:38 -0400 (EDT)
Subject: Re: Cacodylate buffer

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Our major use of cacodylate buffer is for enzyme cytochemistry. Lead
based precipiation reactions are not compatible with phosphate buffers.
However, we also find that for most enzyme cytochemistry cacodylate
buffer gives superior results. For immunocytochemistry, on the other
hand, Gey's salts is our preferred solution.


Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-isnet.is.wfu.edu *
**************************************************************





From: Ilene Sugino :      suginoik-at-UMDNJ.EDU
Date: Fri, 4 Aug 1995 12:50:05 -0400 (EDT)
Subject: staining outer segments

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Hi everyone,

We are having problems staining photoreceptor outer segments in tissue
embedded in JB4. We need to get differential staining of inner and outer
segments for morphometric analysis. We've tried staining with tol blue
which is our standard stain for epon embedded sections. Unfortunately,
we do not get differential staining in JB4.

Does anyone out there have any suggestions? Since this may not be of
general interest to the group, please e-mail me directly.

Thanks

Ilene--



------------------------------------------------------------------------------

Ilene Sugino e-mail: suginoik-at-umdnj.edu
UMDNJ-Ophthalmology phone: (201) 982-7746
DOC 6th Floor fax: (201) 982-7762
90 Bergen Street
Newark, New Jersey 07103

------------------------------------------------------------------------------




From: Marc Brande :      brande-at-SDSC.EDU
Date: Fri, 4 Aug 1995 10:20:33 -0700 (PDT)
Subject: Re: ?Printers: Epson Stylus u...

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microscopy-at-aaem.amc.anl.gov
In-Reply-To: {950804055747_47722794-at-aol.com}
Message-Id: {Pine.3.05.1.9508041032.A2960-a100000-at-pauline.sdsc.edu}
Mime-Version: 1.0
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Where to get the Lexmark?

Marc C. Brande, M.S. SD3D Email List:3D Imaging
San Diego 3D Imaging Group To subscribe/unsubscribe:listserv-at-bobcat.etsu.edu
3840 Camino Lindo To post a message:sd3d-at-bobcat.etsu.edu
San Diego, CA 92122 Email:BRANDE-at-SDSC.EDU Voice:619-587-4830


On Fri, 4 Aug 1995 Microbill-at-aol.com wrote:

} Another solution to the B&W delima is the new Lexmark Optra R which is a
} laser printer that prints at 1200dpi - it's fast and simple -just plug it
} in. They cost about $2500 with the memory necessary to print large 1200 dpi
} images.
}
} Bill Miller
} ElectroImage






From: XiaoGuang Ning :      ningx-at-mcmail.CIS.McMaster.CA
Date: Fri, 4 Aug 1995 13:57:39 -0400 (EDT)
Subject: Information lost on negative films in a JEOL2010F

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Dear Microscopists:

The problem as following is boring me. It will be very kind of you to
give your hands to me if you had experienced such a kind of problem, or can
give me some suggestions.

An FEG JEOL2010F microscope was used. On some exposed negative films,
the information about magnification and scale bar were lost, i.e. they
disappeared at all. However, the information about voltage and text were
still there. I checked the history of the exposed films from the
microscope CRT. There were no the lost information, either.

Your help will be very appreciated.

Best Regards

XiaoGuang NING





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-aaem.amc.anl.gov
Date: Fri, 4 Aug 1995 15:49:18 -0500 (CDT)
Subject: Return of the RR Printer Test

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Dear Colleagues

Many of you will recall that at last years
Computer Workshop & Software Exchange held at the
MSA/MAS meeting in New Orleans we ran a
round robin test of gray scale printers.

I have kept all of the output submitted
by everyone to the Computer Workshop and
will be bringing all those prints with
me back to this years meeting. They were
all stored identically in a file cabinet
for a year. We can all view how well each
of these prints survived.

I would invite everyone that brought a
print to the meeting to once again print
a fresh copy, so that we can compare last
years output with a "fresh" copy.

The test images are available via
Anonymous FTP from the site:

Host: WWW.AMC.ANL.GOV (146.139.72.10)
User: Anonymous
Pass: Your Email Address

The images are in the directory called

7-ImageLibrary

of the ANLSoftWareLibrary

The file names are:

NJZ_MSA_Test_300dpi_MAC (~ 7 Mbytes)
NJZ_MSA_Test_100dpi_MAC (~ 700 Kbytes)
NJZ_MSA_Test_300dpi_IBMPC (~ 7 Mbytes)
NJZ_MSA_Test_100dpi_IBMPC (~ 700 Kbytes)


all are BINARY TIFF files. The _MAC file have Mac Byte order while
the _IBMPC have PC Byte order.

Remember to download them as BINARY!!!!!


See you all in Breckenridge Aug 6-11 (MAS Meeting)
or in KC on August 14th-17th (MSA/HCS Meeting)!!!

I will be at both meetings.

Hopefully, the listserver will run
in relative peace during my absence. However, we all know
that Murphy says the system will go nuts about the time
I get on the plane...


Your Friendly Neighborhood SysOp

Nestor









From: mjr4-at-cornell.edu
Date: Fri, 4 Aug 1995 21:20:34 -0400
Subject: unsubscribe

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unsubscribe microscopy

I've tried being polite by sending this message to
listserver-at-aaem.amc.anl.gov, but
I am still getting microscopy mail. So I am sorry that this list server
does not work
well enough to handle this simple function, forcing me to broadcast such junk.

I find that this list covers too broad a subject area,
and so generates too much mail for which I have no interest. I would rather
subscribe to
a usenet group dedicated just to SEMs.





From: Bob McDonald at RNBCCM28
Date: 8/4/95 3:31PM
Subject: Re: Job Advertisement Re-Write

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August 4, 1995:
Job Opening: Senior TEM Lab Engineer,
Intel Corporation, Santa Clara, California.

----------------------------------------------------------------
Requires two or more years hands-on industrial experience with
the use of Transmission Electron Microscopy and related
techniques in the solution of semiconductor technology problems.
Ideally this will include a number of years of experience
working with development and manufacturing engineers in
process characterization and debug, yield improvement and
failure analysis. Supervision experience and demonstrated
communication and interpersonal skills are necessary.

The individual will carry out TEM and other materials
characterization analyses in response to customer requests, and
will be a key interface with the customers in determining
priorities in an environment where the volume of analytical
requests frequently exceeds the available laboratory resources.
The individual will participate in both task force and more
routine problem solving efforts with our customer base as an
active team member. The individual will interpret and make
recommendations based on lab results and process
knowledge. The individual will be responsible for the development
of improved sample preparation techniques, overall TEM
capability improvement and related analytical method
development. The individual will work with lab peers in
continuous improvement of lab technical capability and
efficiency. The individual will supervise engineers and
technicians in carrying out these responsibilities.


A Ph.D. in Materials Science or equivalent is required.

Intel is an equal opportunity employer.

Please mail resumes to:

John Mardinly
Intel Corporation
2200 Mission College Blvd.
Mail Stop SC2-24
Santa Clara, CA 95052-8119

and/or FAX to:
(408)756-2393

Due to the large number of responses anticipated, there will
generally be no replies unless the applicant is chosen to be a
candidate.




From: zhenquan liu :      zqliu-at-pku.edu.cn
Date: Fri, 4 Aug 1995 10:17:58 -0600 (CST)
Subject: RE: ICXOM E-mail?

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X-Nupop-Charset: English


All and Dr. Sergey Kramar,

Professor Liu Yong Kang is in charge of the 14th International
Congress on X-ray Optics and Microanalysis, which will be held
on August 29 -- September 2, 1995 in Guang Zhou, China.

His fax is : (86) 20 5514 130
His telphone is :(86) 20 5519 755 ext. 2234

He does not have an email service.

I would like to pass all the email messages from the net to him
by fax inside China, if some one uses the net to pass the congress
messages. It would be fast and reliable also. And please do
not worry about the cost of the fax inside China, it is cheap
and not much.

Welcome to China.

Zhen Quan Liu
zqliu-at-pku.edu.cn
Fax: (86) 10 250 1615

In message , writes:

}
} Dear All,
} Does anyone know the E-mail of the secretariat of the ICXOM -
} The 14th International Congress on X-ray Optics and Microanalysis?
} ICXOM will be held on Aug.29 - Sept.2, 1995 in the Guangzhou, China.
} I very need the fast communication with the Organizing Commitee
} and the post is too slow.
} Many thanks!
}
} Sergey Kramar
} CFPM, Moscow Institute of Electronic Technology,
} Moscow 103498, Russia
} E-mail: lemi-at-mx.iki.rssi.ru (to S.Kramar)
} !!!!
} !!!!
} Received: from [162.105.160.2] by pccms.pku.edu.cn with SMTP id AA00464
} (5.67b8/IDA-1.5 for zqliu); Wed, 2 Aug 1995 16:43:43 +0800
} X-Nupop-Charset: English
} Date: Wed, 2 Aug 1995 16:54:48 -0600 (CST)
}




From: ldm2-at-apollo.numis.nwu.edu (L.D.Marks)
Date: Sun, 6 Aug 1995 14:47:35 -0500
Subject: HREM sites

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I am collecting Web links to HREM related and/or active
sites. Please send me by email your link if it is not already
included in the list at http://risc1.numis.nwu.edu/other.html

Thanks




From: ldm2-at-apollo.numis.nwu.edu (L.D.Marks)
Date: Mon, 7 Aug 1995 07:33:13 -0500
Subject: HREM Sites

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I may have inadvertantly given the wrong name when I
requested HREM sites on the Web. The correct location for the
current (incomplete listing) is:
http://risc1.numis.nwu.edu/internet/other.html




From: STEVEN E SLAP :      75767.640-at-compuserve.com
Date: 07 Aug 95 16:48:11 EDT
Subject: Used evaporator available

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Nine years ago, we purchased an Edwards model CR160P/E2M12 evaporator with an
LN2 trap for a specific application. If anyone is interested in it, please call
Paul Kenney at 800-992-9037, or e-mail me directly.
Steven Slap, Energy Beam Sciences





From: STEVEN E SLAP :      75767.640-at-compuserve.com
Date: 07 Aug 95 16:44:01 EDT
Subject: Zeiss Ultraphot II

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We purchased a Zeiss Ultraphot II some years ago, and no longer have any use for
it. It is in good working order, and we have the manual and other
documentation. Please e-mail me directly for further details.
Steven Slap, Energy Beam Sciences





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-aaem.amc.anl.gov
Date: Mon, 7 Aug 1995 21:53:56 -0500 (CDT)
Subject: It never fails!

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G'day Subscribers....

Well I should have known better. If you areall wondering why
there were no positngs for a few days, the system decided to
hang on Sunday. Checking in tonight from the MAS meeting
in Breckenridge Co, I restarted the Mail server. You
should all be getting mail again.


Cheers... Nestor





From: Peter van Aken, Dr., FB11 :      VANAKEN-at-hrz1.hrz.th-darmstadt.de
Date: Mon, 7 Aug 1995 17:34:28 GMT+0200
Subject: Debye temperature of MgO

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Dear everyone,
I'm searching for the Debye temperature of MgO, but I can't find any
reference. I would like to do some temperature dependent EXELFS-
calculations on MgO and compare them to experimental data.

I would be very pleased, if someone could send me a message (Debye
temperature and reference, where to find it in the literature).

Thank you very much in advance


Peter van Aken




From: Peter van Aken, Dr., FB11 :      VANAKEN-at-hrz1.hrz.th-darmstadt.de
Date: Mon, 7 Aug 1995 17:34:28 GMT+0200
Subject: Debye temperature of MgO

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Dr. Peter Antonie van Aken
Institut f|r Mineralogie
Technische Hochschule Darmstadt
Schnittspahnstra_e 9
D-64287 Darmstadt
Germany

E-Mail: VANAKEN-at-hrz1.hrz.th-darmstadt.de

Tel.: [+49] (6151) 16-2180
Fax: [+49] (6151) 16-4021




From: tvoiles-at-unlinfo.unl.edu (Todd Voiles)
Date: Mon, 07 Aug 1995 10:47:31 -0400
Subject: Discussion on EM lab Managing at MSA meeting

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Message-Id: {9508071547.AA00619-at-unlinfo.unl.edu}
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I was just wondering, someone mentioned a roundtable discussion happening at
the MSA meeting in KC next week concerning the managing of EM labs. Is this
still going on?
I have the program book and don't see it anywhere.

Thanks


Todd Voiles
Laboratory Manager
Central Facility for Electron Microscopy
Center for Materials Research and Analysis

University of Nebraska at Lincoln

tvoiles-at-unlinfo.unl.edu





From: ruzin-at-nature.Berkeley.EDU (Steve Ruzin)
Date: Mon, 7 Aug 1995 09:08:08 -0700
Subject: PFA and HCl

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I have read that when making up paraformaldehyde solution one should avoid
HCl when adjusting the pH. Supposedly, HCl reacts with PFA to evolve a
"potent carcinogen". Comments? Could the carcinogen be formaldehyde gas?

Here's the reference.

Jackson, D. (1991). "In-situ hybridization in plants." Molecular Plant
Pathology A Practical Approach Eds Bowles D.J., S.J Gurr and M
McPherson(Oxford University Press.).


Steve...


_______________________________
Steven Ruzin
NSF Center of Plant Developmental Biology
University of California
Berkeley CA 94720-3102
510-642-6602






From: tphillips-at-biosci.mbp.missouri.edu (Tom Phillips)
Date: Mon, 7 Aug 1995 16:56:00 -0600
Subject: V150 Address for Oncor Instruments

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Message-Id: {v01510103ac4c455160d3-at-[128.206.15.189]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

I am looking for the phone number for Oncor Instrument Systems, formerly of
San Diego. I have a seven year old image analysis system they made that is
running great but I need a file conversion program and they are no longer
at their old address. TIA


Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility
3 Tucker Hall
University of Missouri
Columbia, MO 65211
(314)-882-4712 (voice)
(314)-882-0123 (fax)






From: s.griffiths-at-ucl.ac.uk (Stephen Griffiths)
Date: Mon, 07 Aug 1995 09:37:44 +0100
Subject: LM: Photoconversion of DiI

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We have some brain material stained with DiI. The fluorescence fades very
quickly with this stain.

We are attempting by photoconversion, to obtain a permanent reaction
product, using DAB and the microscope's fluorescent lamp.

The results are very inconsistent. Sometimes it works, sometimes not.
Usually not.

Does anyone have any experience of this technique? If so do you know how, or
indeed whether it is possible, to obtain reliable results?

TIA.

Regards
Stephen Griffiths.

{} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {}
{} Stephen Griffiths {} e-mail s.griffiths-at-ucl.ac.uk {}
{} Visual Science Department {} {}
{} Institute of Ophthalmology {} Tel: 0171 608 6914 {}
{} London. EC1V 9EL. UK. {} Fax: 0171 608 6850 {}
{} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {}





From: CAROLYN J. EMERSON, DEPT. OF BIOLOGY, MEMORIAL UNIVERSITY
Date: Mon, 07 Aug 1995 14:57:30 -0230
Subject: Specimen holder - Zeiss EM9A

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Sender: cemerson-at-KEAN.UCS.MUN.CA
{cemerson-at-kean.ucs.mun.ca}
Reply-To: cemerson-at-KEAN.UCS.MUN.CA
To: MICROSCOPY-at-AAEM.AMC.ANL.GOV
Message-ID: {0099485E.950F3644.4905-at-leif.ucs.mun.ca}

Our lab owns a Zeiss EM 9A TEM purchased in the mid 60's. It is still
functioning well, with a few quirks of personality here and there (as
with us all!!), but we've recently had some damage to our specimen holders.
We were wondering if anyone out there haas an old Zeiss EM 9A that is being
cannibalized or is ready for the scrap heap and has some parts to spare.
Specifically, we would like to scrounge or buy a specimen holder. If you
have one, could you please contact me off-line with the details? Thank you.
Carolyn J. Emerson
Dept. of Biology
Memorial Univ. of Newfoundland
St. John's, NF, Canada A1B 3X9

email: cemerson-at-kean.ucs.mun.ca
Tel: 709-737-7515
Fax: 709-737-3018




From: editor-at-microscopy-online.com (Editor, Microscopy Online)
Date: Tue, 8 Aug 1995 00:08:57 -0700
Subject: Microscopy Online Web Server Announcement

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Message-Id: {v01520d00ac4cbd61a33c-at-[165.247.24.148]}
Mime-Version: 1.0
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Microscopy Online is happy to announce its server on the WWW at

http://www.microscopy-online.com/

Microscopy Online is a hyper-journal containing information and
features of interest to the microscopy community, including articles,
job listings, calendar of events, keyword-searchable buyer's guide,
showcase of new products and services, list of recent publications,
used equipment for sale, etc.

We invite to you take a look and welcome your comments and ideas
for improvements.

- Editor, Microscopy Online






From: Anna Carlsson :      OO2ANNA-at-robin.mbfys.lth.se
Date: Tue, 08 Aug 1995 12:54:00 +0200
Subject: RE:Text on negatives

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Regarding the problem with disappearing scalebar and maginfication on
negatives:
We have had the same problem with our JEM4000-EX for over a year now. It
started when we installed a Gatan CCD-camera with Digital Micrograph and
autoalignment. The scalebar and mag. sometimes disappears during a session,
but it doesn't seem to be any special event that causes it. However, the
annotation usally comes back when we reset the microscope computer. Jeol
Scandinavia has so far not been able to solve the problem.

I don't know if this is of any help, but at least you know that your not
the only one having this problem.

Best Regards
Anna Carlsson
National Center for HREM
Lund University, Sweden




From: Beverly E Maleeff :      Beverly_E_Maleeff%notes-at-sb.com
Date: 8 Aug 95 10:01:55 EDT
Subject: Re: Discussion on EM lab Managing at MSA meeting

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Todd et al.:
The Technologists' Forum roundtable, entitled "Survival Part III: What Works
and What Doesn't", will be held Thursday from 1-3 PM in Room 1201 at the
Convention Center. It is on page 45 of the program.
If you have any questions, please contact me this week, or come to the Tech
Forum booth in the exhibit hall at the meeting. Hope to see you there.

Bev Maleeff

Todd Voiles wrote:
I was just wondering, someone mentioned a roundtable discussion happening at
the MSA meeting in KC next week concerning the managing of EM labs. Is this
still going on?
I have the program book and don't see it anywhere.

Thanks


Todd Voiles
Laboratory Manager
Central Facility for Electron Microscopy
Center for Materials Research and Analysis

University of Nebraska at Lincoln

tvoiles-at-unlinfo.unl.edu





From: Jay Jerome :      jjerome-at-isnet.is.wfu.edu
Date: Tue, 8 Aug 1995 12:04:37 -0400 (EDT)
Subject: RE: Re: Cacodylate buffer

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After my post on cacodylate buffer, I have had a number of people ask me
what is Gey's salts. So I thought I would share this information with the
entire list.
Gey's salts is a balanced salt solution, similar to Hank's. A recipe can
be found in most tissue culture handbooks. We have found that preserves
antigenicity in most proteins better than EM buffers or other balanced
salt solutions. It has only a limited buffering capability, so pHing can
be difficult. We use very dilute NaOH and HCl to alter it's pH.

Hope this helps.


Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-isnet.is.wfu.edu *
**************************************************************

On Tue, 8 Aug 1995, PRING wrote:

} I note your comments on cacodylate - what are grey's salts?
}
} Richard
}
} richard.pring-at-bbsrc.ac.uk
}
} Richard Pring
} Long Ashton Research Station
} Long Ashton
} Bristol
} UK
}




From: PALMER-at-ecs.umass.edu
Date: Tue, 08 Aug 1995 13:34:06 -0500
Subject: TEM sample prep - films on sapphire substrates

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I'm looking for any advice or references on making plan-view (or cross section)
specimen of films grown on sapphire. From what I've read, the samples can
be make using tried and true methods used commonly on semiconductor substrates
(polish/dimple/ion mill). Does any one know any helpful hints or 'tricks'
to make this process go as smoothly as possible? I am also thinking of
chemical etching for plan-view specimen, but I have the usual problem of
how to know when to stop etching. Any suggestions?

Joyce Palmer
University of Massachusetts
ECE department
Amherst, Ma. 01003
413-545-4647





From: howelld-at-egr.msu.edu (David Howell)
Date: Tue, 8 Aug 1995 13:40:22 -0400 (EDT)
Subject: 1200DPI laserprinter info

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First, my apologies to those who read the NIH-image listserver,
as this is a duplicate of a posting I made there earlier
today.

I am collecting information on 1200dpi monochrome laserprinters
for printing half-toned grayscale images of scanned TEM and
HRTEM photographic prints. We want the capability to print
output from Photoshop and NIH-Image onto paper and
transparencies for seminars and progress reports without
degrading the image quality. Presently, we resort to reproducing
the photographs with the monochrome mode on a Cannon color
copier to preserve as many gray levels in the final output.
This approach, however, does not allow direct output from
the computer, which in our case is a PowerMac 7100/80.

I would appreciate responses from anyone who has had any
experience with the following 1200dpi laserprinters:

QMS 1660E
Lexmark Optra R
Xante Accel-a-writer (need phone number)
GCC Selectpress 1200

I would also appreciate any pointers to review articles,
comments about gray-scale printing, interface pitfalls, and
other 1200dpi models available (Please include phone # or web sites).

Thank You,

David A. Howell
MSM Dept.
Michigan State University
E. Lansing, MI
48824-1226




From: bozzola-at-siu.edu (John. J. Bozzola)
Date: Tue, 8 Aug 1995 13:57:54 -0600
Subject: Re: Discussion on EM lab Managing at MSA meeting

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The rountable on EM Facility Management will be on Thursday, 1-3PM, August
16, Room 1201. Topic: Survival Part Three: What Works and What Doesn't.

--------------------------
Earlier, Tod Voiles Wrote:

} I was just wondering, someone mentioned a roundtable discussion happening at
} the MSA meeting in KC next week concerning the managing of EM labs. Is this
} still going on?
} I have the program book and don't see it anywhere.
}
--------------------------

#############################################################################
John J. Bozzola, Director
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
#############################################################################






From: varello-at-medcolpa.edu
Date: Tue, 08 Aug 1995 15:45:24 -0400
Subject: xray microanalys

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Are there any new methods available for biological specimen preparations
for xray analysis for SEM?

Michael A Varello
varello-at-medcolpa.edu





From: richard.easingwood-at-stonebow.otago.ac.nz (Richard Easingwood)
Date: Wed, 9 Aug 1995 09:20:23 +1100
Subject: Lowicryl resins

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To users of Lowicryl resins:
I have just opened two kits of Lowicryl K11M, both with the same lot number
from the same supplier.
In one of the kits the monomer is a pale straw colour (abnormal) while in
the other kit the monomer is colourless (normal). The pale straw colour is
a bit disconcerting as I am used to the colourless momomer. In addition the
blocks made from the pale straw coloured monomer seem to polymerise
slightly harder than the colourless ones however I have no idea if the
discoloured resin effects immunoreactivity.
To date Polysciences have not been able to explain the colour difference.
Has anyone out there had the same experience? If so do you have any ideas
on what causes it? Do you think it affects immunoreactivity?

Allan Mitchell




Please reply to: richard.easingwood-at-stonebow.otago.ac.nz

Richard Easingwood
South Campus Electron Microscope Unit
Otago Medical School
PO Box 913
Dunedin
NEW ZEALAND

Telephone: 64-03-479 7301
Facsimile: 64-03-479 7254

The southernmost electron microscope unit in the world






From: Miguel Avalos B. :      miguel-at-ifuname.ifisicaen.unam.mx
Date: Tue, 8 Aug 1995 15:49:13 -0700
Subject: Re: Zeiss Ultraphot II

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From: richard.easingwood-at-stonebow.otago.ac.nz (Richard Easingwood)
Date: Wed, 9 Aug 1995 12:20:13 +1100
Subject: TEM:platelet cryofixation experiences

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We are about to start an experiment which involves human platelets for a
user of the Unit. He wants to look at the platelet structure before
aggregation occurs. Aggregation of platelets can occur during platelet
isolation but in particular during chemical fixation. I am proposing to
cryofix and cryosubstitute the platelets. Has anybody had experience with
platelets and this technique who would like to share that experience?

Many thanks,

Allan Mitchell

Please reply to: richard.easingwood-at-stonebow.otago.ac.nz

Richard Easingwood
South Campus Electron Microscope Unit
Otago Medical School
PO Box 913
Dunedin
NEW ZEALAND

Telephone: 64-03-479 7301
Facsimile: 64-03-479 7254

The southernmost electron microscope unit in the world






From: David Dryden :      djd-at-electron.ph.unimelb.edu.au
Date: Wed, 9 Aug 1995 14:05:52 +1000
Subject: Microscopy On-line

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Comments: Converted from PROFS to RFC822 format by PUMP V2.2X



Dear Fellow Microscopists,
Could someone please forward the recent listing
concerning the microscopy-online service as seen by this
listserver.
with thanks David Dryden
School of Physics
University of Melbourne
Australia
djd-at-electron.ph.unimelb.edu.au




From: Mike Witcomb :      MIKEW-at-gecko.biol.wits.ac.za
Date: Wed, 9 Aug 1995 10:19:44 GMT+2
Subject: Jet polishing of gold alloy

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I would be grateful of any advice on the best way to polish, in a
Fischione twin jetpolisher, a Au-Cu-Al alloy in which gold is present
at about 50%atomic.
Thanks


Dr MJ Witcomb
Electron Microscope Unit
University of the Witwatersrand
Private Bag 3
WITS
2050
South Africa

Telephone: + 27 11 716 4000
+ 27 11 716 2419 (messages)
Fax: + 27 11 339 3407
E-mail: mikew-at-gecko.biol.wits.ac.za




From: L.S. SMITH :      MTLLSS-at-ECU-01.NOVELL.LEEDS.AC.UK
Date: Wed, 9 Aug 1995 10:02:01 GMT
Subject: Cold Stage for Ion Tech wanted

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Dear Readers,

I am currently using an old Ion Tech B306 ion mill to produce foils for
TEM microanalysis. I would like to ion mill specimens at liquid
nitrogen temperatures but, unfortunately, I do not have access to a cold
stage. Furthermore, my budget is constrained and will not stretch to
the cost of buying a new one. I would, however, be interested in
hearing from anyone who has an Ion Tech cold stage which is
superflous to their requirements and which they would be willing to
sell (or even perhaps part exchange for other Ion Tech Parts).

Thank you,
***************************************
************ Lee Smith ************
******* School of Materials *******
******* University of Leeds *******
******* Leeds, LS2 9JT, UK *******
***************************************




From: Ilene Sugino :      suginoik-at-UMDNJ.EDU
Date: Wed, 9 Aug 1995 08:33:22 -0400 (EDT)
Subject: film scanner

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Hi everybody,

Does anyone know if there exists a high resolution film scanner that will
scan 31/4x4 and 4x5 negatives in addition to 35mm? I have a brochure
for the Nikon LS-3510AF and it looks like the largest negative it will
scan is 40mmx40mm.

If such a scanner does exist, what is the quality of the scanned image?
I would like to output the scanned image (after manipulation in
Photoshop) to a Codonics dye-sublimation color printer or film recorder.

Thanks for any input.

Ilene--

------------------------------------------------------------------------------

Ilene Sugino e-mail: suginoik-at-umdnj.edu
UMDNJ-Ophthalmology phone: (201) 982-7746
DOC 6th Floor fax: (201) 982-7762
90 Bergen Street
Newark, New Jersey 07103

------------------------------------------------------------------------------




From: Peter Goodhew :      goodhew-at-liverpool.ac.uk
Date: Wed, 9 Aug 1995 14:29:10 BST
Subject: STEM workshop

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High resolution STEM: An informal workshop

11th September 1995 at Department of Materials Science &
Engineering, University of Liverpool, UK

This workshop is timed to take place immediately before EMAG 95 at
Birmingham (12th-15th September). It provides an unique
opportunity to see a VG HB601UX high resolution analytical STEM in
operation and to discuss problems with regular users of the
instrument.

There will be four sessions, and plenary lectures by speakers from
the USA, UK and Germany, including Steve Pennycook on Z-contrast
imaging and and Ian Vatter on high spatial resolution analysis.

You can register (there is no fee!) via Anne Leonard at Fisons
(44) 1342 327211, Fax (44) 1342 300515 or via the Fisons Web page
at http://www.surface.fisons.co.uk/workshop

The instrument at Liverpool is the North-West STEM, jontly run by
the Universities of Manchester and Liverpool.

Peter Goodhew



----------------------------------------------------------------
Professor Peter J Goodhew
Department of Materials Science & Engineering
University of Liverpool
LIVERPOOL Fax (44) (0)151 794 4675
L69 3BX, UK Tel (44) (0)151 794 4665 (secretary Debra)
----------------------------------------------------------------
inter alia:

Director of the MATTER project for educational software
Web page: http://www.liv.ac.uk/~matter/home.html
Tel (44) (0)151 794 5006 (secretary Jean)

Dean of Engineering Tel (44) (0)151 794 4920 (secretary Carol)
Fax (44) (0)151 794 4930
----------------------------------------------------------------






From: tvoiles-at-unlinfo.unl.edu (Todd Voiles)
Date: Wed, 09 Aug 1995 08:56:37 -0400
Subject: Re: film scanner

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} Hi everybody,
}
} Does anyone know if there exists a high resolution film scanner that will
} scan 31/4x4 and 4x5 negatives in addition to 35mm? I have a brochure
} for the Nikon LS-3510AF and it looks like the largest negative it will
} scan is 40mmx40mm.
}
} If such a scanner does exist, what is the quality of the scanned image?
} I would like to output the scanned image (after manipulation in
} Photoshop) to a Codonics dye-sublimation color printer or film recorder.
}
} Thanks for any input.
}
}
There are plenty of commercially available 1200 dpi and even
2400 dpi scanners out there for 1-5k. Is this enough resolution for what
you want to do? They can usually scan in excess of
8x10 inches.


Todd Voiles
Laboratory Manager
Central Facility for Electron Microscopy
Center for Materials Research and Analysis

University of Nebraska at Lincoln

tvoiles-at-unlinfo.unl.edu





From: chandler-at-lamar.ColoState.EDU (John Chandler)
Date: Wed, 9 Aug 1995 08:57:49 -0600
Subject: Re: film scanner

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} Hi everybody,
}
} Does anyone know if there exists a high resolution film scanner that will
} scan 31/4x4 and 4x5 negatives in addition to 35mm? I have a brochure
} for the Nikon LS-3510AF and it looks like the largest negative it will
} scan is 40mmx40mm.
}
} If such a scanner does exist, what is the quality of the scanned image?
} I would like to output the scanned image (after manipulation in
} Photoshop) to a Codonics dye-sublimation color printer or film recorder.
}
} Thanks for any input.
}
} Ilene--

One film scanner to look at is the Leafscan 45, which does any size up to
4X5. It is a bit pricey, $15K when we looked a year ago, but it does a
very nice job. It uses the same round negative carriers as Beseler
enlargers, which are cheaper than the Leaf brand.

I don't remember the resolution, but, if it weren't for the price, I would
have pushed for getting this scanner for our TEM negatives. It has
automatic exposure control that gives very good results.

I'd like to see a vendor do some demos of these devices at MSA in Kansas
City. Any takers from the vendors out there?

John
chandler-at-lamar.ColoState.EDU






From: REEVE008-at-mc.duke.edu
Date: Wed, 09 Aug 1995 10:45 -0400 (EDT)
Subject: LM: Digital Cameras

Contents Retrieved from Microscopy Listserver Archives
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Registered-mail-reply-requested-by: REEVE008-at-mc.duke.edu


I am investigating the feasibility of purchasing a digital camera
for use in our department to put on a microscope (probably an
Olympus Vanox) and am trying to understand the advantages/disadv. of
a dig. camera over video or scanning 35mm slides since the cost seems
to be so high.

1) Video vs. digital cameras: Video CCD cameras can be put on
microscopes and can capture a pretty good quality image, requiring a
video capture board for use on the computer. The image quality is
limited, however, by the resolution of the CRT. The digital cameras
should be able to improve on the resolution in theory , but what does
the image actually look like? Fuji says their image only holds up to
a 3x5 enlargement... Is video still the better alternative for
instant file use, and scanning 35mm slides still the best quality?


2) Digital Recommendations: Fuji says their camera isn't really
ready for use on a scope, largely due to the incompatibility of the
design of light capture in a digital system and the idiosyncrasies of
a microscope, specifically the camera's reliance on the autofocus
lens for metering versus an objective and the camera's limit of ISO
values of only 800 and 1600. So has anyone used a Kodak camera on a
scope, or any other brand? What were the successes and problems with
those you've tried? Is file format (.jpeg or .tif versus a
proprietary format that needs to be converted for use in a real
application) an issue that has been a problem? What are you doing
for storage? Kodak's Digital imaging helpdesk has not been able to
link me up yet w/ anyone in the company that knows anything about
their cameras on a microscope...


Any comments or light that you can shed on this subject would be
greatly appreciated. If it would be easier for you to talk with me on
the phone, please send your phone number and I'll call you. THanks
in advance for your time to reply -


Susan Reeves
Supervisor, PhotoPath
DUMC Department of Pathology
reeve008-at-mc.duke.edu
919-684-3984









From: Ilene Sugino
Date: 8/9/95 6:34 AM
Subject: Re: film scanner

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Message-Id: {n1404160428.85431-at-macmail7.lbl.gov}
"Microscopy" {Microscopy-at-aaem.amc.anl.gov}
X-Mailer: Mail*Link SMTP/QM 3.0.0

Reply to: RE} film scanner

Ilene:
We use a LeafScan-45 for negatives from 35mm to 4"x5" controlled by a Mac (via SCSI, but you could use GPIB). It uses standard
Beseler enlarger film carriers, has a plug-in for Photoshop, and scans up to 6000x12000 pixels (216MB file for color -- 72MB for
grayscale!). The maximum dpi is 4000, so the minimum size to get 6000 pixels across is 1.5" (if you want to scan (say) a 10mmx10mm
area, you get only 1575x1575 pixels). We bought the LeafScan (from Leaf Systems: 508-460-8300) as a replacement for an Eikonix
78/99, and are very satisfied with it.
Mike O'Keefe
--------------------------------------

Does anyone know if there exists a high resolution film scanner that will
scan 31/4x4 and 4x5 negatives in addition to 35mm? I have a brochure
for the Nikon LS-3510AF and it looks like the largest negative it will
scan is 40mmx40mm.

If such a scanner does exist, what is the quality of the scanned image?
I would like to output the scanned image (after manipulation in
Photoshop) to a Codonics dye-sublimation color printer or film recorder.

Thanks for any input.

Ilene--

------------------------------------------------------------------------------

Ilene Sugino e-mail: suginoik-at-umdnj.edu
UMDNJ-Ophthalmology phone: (201) 982-7746
DOC 6th Floor fax: (201) 982-7762
90 Bergen Street
Newark, New Jersey 07103

------------------------------------------------------------------------------






From: David Henriks :      73531.1344-at-compuserve.com
Date: 09 Aug 95 13:51:08 EDT
Subject: Jet polishing of gold alloy

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While I cannot give you any help with using a Fischione Polisher, i can give you
a recipe which is used with the South Bay Technology Model 550. Perhaps you can
use this as a basis for a Fischione recipe.

Reference:
B.J. Kestel, "Jet Thinning of YBaCuO High Tc Superconducotr and also Gold for
TEM with a Non-Acid Electrolyte" Ultramicroscopy 25 (1988) pp 351-354.

If you cannot get a copy, I can send one to you.

He uses a BK-2 Solution which is:
5.3g lithium chloride
11.16g magnesium perchlorate
100ml butyl cellosolve
500 ml methanol

Polishing was done at -55C with a potential of 150V at one half the maximum flow
rate.

I hope this helps. If you would like anyu additional information or a copy of
the paper, please let me know.

Best regards-

David Henriks TEL: 800-728-2233 (toll-free in USA)
South Bay Technology, Inc. 714-492-2600
1120 Via Callejon FAX: 714-492-1499
San Clemente, CA 92673 USA e-mail: 73531.1344-at-compuserve.com





From: David Henriks :      73531.1344-at-compuserve.com
Date: 09 Aug 95 13:52:44 EDT
Subject: MSA Tutorial on Tripod Polishing/Ion Milling

Contents Retrieved from Microscopy Listserver Archives
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ROGER ALVIS {Roger.Alvis-at-amd.com} ,
"Ronald M. Anderson" {ron-anderson-at-vnet.ibm.com} ,
"Miguel Avalos B." {miguel-at-ifuname.ifisicaen.unam.mx} ,
Yolande Berta {YBerta-at-matreng.courier.gatech.edu} ,
Peggy Bisher {peggy-at-research.nj.nec.com} ,
henk colijn {colijn-at-kcgl1.eng.ohio-state.edu} ,
Russ Cook/Argonne {COOK-at-aaem.amc.anl.gov} ,
Mike Dibattista {MikeDib-at-engine.umich.edu} ,
Cindy Dogan {DOGAN-at-alrc.usbm.gov} ,
Estevez {estevez-at-atp6000.tuwien.ac.at} ,
Phil Flaitz-IBM {pflaitz-at-vnet.ibm.com} , Tim Foecke {tfoecke-at-nist.gov} ,
Richard Fonda {Fonda-at-anvil.nrl.navy.mil} ,
Chuck Garber {GVKM07A-at-prodigy.com} , Zack Gemmill {zack-at-lsil.com} ,
Lucille Giannuzzi {lag-at-pegasus.cc.ucf.edu} ,
Ed Goo {ekgoo-at-mizar.usc.edu} , Peter Goodhew {goodhew-at-liv.ac.uk} ,
Vidya Kaushik {QJXNJ21-at-memrqa.sps.mot.com} ,
Bernie Kestel {Bernard_Kestel-at-QMGATE.ANL.COM} ,
"Kim,SungTae(T:4551)" {STKIM-at-gscrl.goldstar.co.kr} ,
Michael Lamvik {mlamvik-at-mcnc.org} ,
"J.S. Lee" {JSLEE-at-gscrl.goldstar.co.kr} ,
Tan Chen Lee-Cornell {tanchen-at-msc.cornell.edu} ,
Microscopy ListServer {MICROSCOPY-at-aaem.amc.anl.gov} ,
Zhen Quan Liu {zqliu-at-pku.edu.cn} ,
Jian Shu Luo {jian_shu_luo-at-qmgate.anl.gov} ,
Charlie Lyman {cel1-at-lehigh.edu} ,
Jordi Marti {MartiJ-at-mtomp201.research.allied.com} ,
James McCormick {JAMESM-at-teetot.acusd.edu} ,
Stuart McKernan {mckernan-at-cems.umn.edu} ,
"F. Scott Miller" {smiller-at-umr.edu} ,
Lucio Mulestagno {LucioM-at-newton.umsl.edu} ,
Judy Murphy/SJDC {murphy-at-ms.sjdccd.cc.ca.us} ,
BOB ROBERTS {ROBERTS-at-csss.la.asu.edu} ,
Ludo Rossou {LUDO_GERTIE-at-ematserv.ruca.ua.ac.be} ,
Jake Schaper {Jake_Schaper-at-chdqm.sps.mot.com} ,
David Su {davidsu-at-aol.com} , Changmo Sung {Sungc-at-aspen.uml.edu} ,
Don Grimes/Micro Today {MicroToday-at-aol.com} ,
Scott Walck {WALCKSD-at-ml.wpafb.af.mil} ,
John Wheatley {WHEATLEY-at-csss.la.asu.edu}

South Bay Technology will present a tutorial on Tuesday night from 5-7pm in
their exhibit booth (no. 619-621). The tutorial is FREE. All you need to do is
register for it at the convention center. Look for the signs announcing Vendor
Tutorials. If you have trouble figuring out how to register, just stop by our
booth on Monday or Tuesday and we'll get you set up.

Presenters:

Trpiod Polishing: Shane Roberts
Applications Engineer
South Bay Technology, Inc.

Ion Milling: Dr. Arpad Barna
Research Institute of Technical Physics
Budapest, Hungary

"South Bay Technology will discuss the fundamentals of Tripod Polishing
including a step by presentation of sample mounting, sample alignment, Tripod
Polisher calibration, 1st side polishing and 2nd side (wedge) polishing. New
sample mounts and accessories will also be introduced during the tutorial. The
newest advances in Tripod Polishing will also be discussed which makes this
tutorial ideal for both novices and experienced Tripodders. The ion milling
portion of the tutorial will be an introduction to the IV3 Ultra Low Angle Ion
Mill. The 1st production version of the IV3 was introduced in Hungary in 1987.
Since that time the IV3 has enjoyed brisk sales throughout Europe and the Far
East. This tutorial is an opportunity to learn more about the unique ion guns
and the many benefits associated with its retarding field capability. Dr. Barna
is a world reknowned expert on ion milling and developed the first working model
of the IV3 in his lab in 1982."





From: David Henriks :      73531.1344-at-compuserve.com
Date: 09 Aug 95 13:53:36 EDT
Subject: Tripod Polisher User's Group Meeting

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ROGER ALVIS {Roger.Alvis-at-amd.com} ,
"Ronald M. Anderson" {ron-anderson-at-vnet.ibm.com} ,
"Miguel Avalos B." {miguel-at-ifuname.ifisicaen.unam.mx} ,
Yolande Berta {YBerta-at-matreng.courier.gatech.edu} ,
Peggy Bisher {peggy-at-research.nj.nec.com} ,
henk colijn {colijn-at-kcgl1.eng.ohio-state.edu} ,
Russ Cook/Argonne {COOK-at-aaem.amc.anl.gov} ,
Mike Dibattista {MikeDib-at-engine.umich.edu} ,
Cindy Dogan {DOGAN-at-alrc.usbm.gov} ,
Estevez {estevez-at-atp6000.tuwien.ac.at} ,
Phil Flaitz-IBM {pflaitz-at-vnet.ibm.com} , Tim Foecke {tfoecke-at-nist.gov} ,
Richard Fonda {Fonda-at-anvil.nrl.navy.mil} ,
Chuck Garber {GVKM07A-at-prodigy.com} , Zack Gemmill {zack-at-lsil.com} ,
Lucille Giannuzzi {lag-at-pegasus.cc.ucf.edu} ,
Ed Goo {ekgoo-at-mizar.usc.edu} , Peter Goodhew {goodhew-at-liv.ac.uk} ,
Vidya Kaushik {QJXNJ21-at-memrqa.sps.mot.com} ,
Bernie Kestel {Bernard_Kestel-at-QMGATE.ANL.COM} ,
"Kim,SungTae(T:4551)" {STKIM-at-gscrl.goldstar.co.kr} ,
Michael Lamvik {mlamvik-at-mcnc.org} ,
"J.S. Lee" {JSLEE-at-gscrl.goldstar.co.kr} ,
Tan Chen Lee-Cornell {tanchen-at-msc.cornell.edu} ,
Microscopy ListServer {MICROSCOPY-at-aaem.amc.anl.gov} ,
Zhen Quan Liu {zqliu-at-pku.edu.cn} ,
Jian Shu Luo {jian_shu_luo-at-qmgate.anl.gov} ,
Charlie Lyman {cel1-at-lehigh.edu} ,
Jordi Marti {MartiJ-at-mtomp201.research.allied.com} ,
James McCormick {JAMESM-at-teetot.acusd.edu} ,
Stuart McKernan {mckernan-at-cems.umn.edu} ,
"F. Scott Miller" {smiller-at-umr.edu} ,
Lucio Mulestagno {LucioM-at-newton.umsl.edu} ,
Judy Murphy/SJDC {murphy-at-ms.sjdccd.cc.ca.us} ,
BOB ROBERTS {ROBERTS-at-csss.la.asu.edu} ,
Ludo Rossou {LUDO_GERTIE-at-ematserv.ruca.ua.ac.be} ,
Jake Schaper {Jake_Schaper-at-chdqm.sps.mot.com} ,
David Su {davidsu-at-aol.com} , Changmo Sung {Sungc-at-aspen.uml.edu} ,
Don Grimes/Micro Today {MicroToday-at-aol.com} ,
Scott Walck {WALCKSD-at-ml.wpafb.af.mil} ,
John Wheatley {WHEATLEY-at-csss.la.asu.edu}

First let me say that this user's group meeting is not limited to those of you
who have purchased a Tripod Polisher from South Bay Technology. We welcome
ANYONE who has an interest in Tripod Polishing.

I'm sorry it has taken so long to get these final details on the User's Group
Meeting. I hope you all took my advice and reserved the time in your schedule!
We will meet as follows:

WHEN: Monday August 14th at 6:00 pm

WHERE: Gino Schiraldi's (Pizza Place)
323 W. 8th Street
Kansas City, MO 64105
TEL: 816-421-2211

Gino's is located about 4 blocks from the convention center. Walk down Central
to 9th and make a left. Then a quick right on May. Go 1 block to 8th and turn
right. Gino's will be right there.

South Bay Technology will provide pizza and beer - so come hungry! It would
also help me a great deal if you could RSVP. I'd like to get a rough idea of
how many people will attend so we can reserve a large enough area.

It would be great if you could bring with you any photographs of your work,
examples of Tripod Polisher modifications, copies of papers or procedures you
have written etc. If you need (or want) to get in touch with me in Kansas City
you can give me a call at the Marriott (816-421-6800). I'll arrive Sunday
evening..

PLEASE let others know about the User's Group Meeting. I know I'm late in
getting this information out so I'll need your in publicizing it.

Also, SBT will present a vendor tutorial on Tuesday night from 5-7 in the
exhibit hall. The topics will be Tripod Polishing and Ultra Low Angle Ion
Milling. The Ion Milling discussion will be led by Dr. Arpad Barna from the
Research Institute for Technical Physics in Budapest, Hungary. He will discuss
the IV3 Ion Mill which is being introduced to the U.S. market at MSA. The
tutorial will be held in the South Bay Technology booth (619-621). We hope to
see you there!

Best regards-

David Henriks TEL: 800-728-2233 (toll-free in USA)
South Bay Technology, Inc. 714-492-2600
1120 Via Callejon FAX: 714-492-1499
San Clemente, CA 92673 USA e-mail: 73531.1344-at-compuserve.com





From: Marc Brande :      brande-at-SDSC.EDU
Date: Wed, 9 Aug 1995 11:38:06 -0700 (PDT)
Subject: Digital Imaging of Live Cells

Contents Retrieved from Microscopy Listserver Archives
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MolecularCellSpeak List {molecular-cell-speak-at-mailbase.ac.uk} ,
Microscopy List {microscopy-at-aaem.amc.anl.gov} ,
Info-Bios List {info-bios-at-mom.spie.org} ,
Confocal Microscopy List {confocal%ubvm.BITNET-at-BITNIC.CREN.NET} ,
Cell Bio List {cellbiol-at-net.bio.net} ,
Biz-Biotech List {biz-biotech-at-netcom.com} ,
Bionews List {bionews-at-net.bio.net} , Biomaterial List {biomat-l-at-hearn}
Message-Id: {Pine.3.05.1.9508091106.A25102-b100000-at-pauline.sdsc.edu}
Mime-Version: 1.0
Content-Type: TEXT/PLAIN; charset=US-ASCII

Is Your Unique Cell Image Data going Unrecorded?

There is a Solution!

Digital Imaging of Your Live Cells.

Capture Important
Structures
Functions
and
Interactions
of
Your Live Cells In Situ!

Monitor and Document Your Live-Cell Experiments Routinely and Frequently.

Why Digital Imaging?

Digital Images can be:
Displayed
Manipulated
Processed
Stored
Retrieved
Shared
Transported

All via your Personal Computer!

No need to invest in expensive system hardware and imaging personnel

Ease of Use
Safe Archival Image Storage
Economical
Fast Turnaround
Performed by Live-Cell Imaging Biologist
Meticulous Care Taken with Your Live Cells
Complete Confidentiality Assured

To image your live cells, contact:

Marc C. Brande, M.S. or
Caroline Yu at
Cell Applications, Inc.
Toll-Free: 1-800-645-0848
Local: 619-453-0848
Fax: 619-453-2862
Email: BRANDE-at-SDSC.EDU

Marc C. Brande, M.S. SD3D Email List:3D Imaging
San Diego 3D Imaging Group To subscribe/unsubscribe:listserv-at-bobcat.etsu.edu
3840 Camino Lindo To post a message:sd3d-at-bobcat.etsu.edu
San Diego, CA 92122 Email:BRANDE-at-SDSC.EDU Voice:619-587-4830





From: Fred Hayes :      fahayes-at-ucdavis.edu
Date: Wed, 9 Aug 1995 12:38:05 -0700 (PDT)
Subject: rabbit cornea fixation

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Customer is interested in drug toxicity of rabbit cornea. Is it best to
fix in 3% Glutaraldehyde only or Glutaraldehyde (2.5%) and
Paraformaldehyde (1.5%)? This is an anterior chamber study including the
cornea endothelial cell layer.

Many thanks.

Fred A. Hayes
Dixon, CA
916-678-6280





From: XiaoGuang Ning :      ningx-at-mcmail.CIS.McMaster.CA
Date: Wed, 9 Aug 1995 16:43:44 -0400 (EDT)
Subject: Re:MgO Debye temperature

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Hi, there:

The calculated Debye temperature of MgO is 630K, which can be found in
{ {The Oxide Handbook} } , 2nd edition, Ed. by G.V.Samsonov,
published by IFI/Plenum Data Company (1982), p.109.

No experimental data of Debye Temp. for MgO is available in the book.

Good luck!

X.G.Ning




From: jester-at-crnjjsgi.swmed.edu (James V. Jester)
Date: Wed, 09 Aug 1995 17:24:07 -0500
Subject: Position

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Alternate-Recipient: allowed
Auto-Forwarded: prohibited
Content-Return: allowed
Disclose-Recipients: prohibited
Conversion: allowed
Importance: normal
Priority: normal
Sensitivity: Company-Confidential


POST-DOCTORAL POSITION IN BIOMEDICAL IMAGING:

Applications are invited for a full-time position as a
Research Associate in the Department of Ophthalmology at the
University of Texas Southwestern Medical Center at Dallas.
We are seeking an individual with interest and experience in
biological microscopy and computer imaging. The ideal
candidate will have a Ph.D. in Biomedical Engineering, Cell
Biology or related discipline. The proposed work will focus on the
acquisition and quantitation of 3- and 4-dimensional images
obtained from patients and experimental tissue using confocal
microscopy. Responsibilities will include digitizing,
processing, interpreting and analyzing images, as well as developing
software as needed in order to generate reliable quantitative data.
We use Silicon Graphics Workstations for image analysis (C or C++
programming languages).

Position is available immediately.
Applicants should send a curriculum vitae with statement of interest to:

Dr. W. Matthew Petroll
Department of Ophthalmology
University of Texas Southwestern Medical Center at Dallas
5323 Harry Hines Blvd.
Dallas, TX 75235-9057
FAX: 214-648-2382
EMAIL: petroll-at-crnmpsgi.swmed.edu

UT Southwestern is an equal opportunity employer.





From: Jay Jerome :      jjerome-at-isnet.is.wfu.edu
Date: Wed, 9 Aug 1995 21:40:15 -0400 (EDT)
Subject: Re: cutting thermanox

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We have often cut cells still on thermanox coverslips. We use an older
knife to be on the safe side, but they seem to cut o.k. The only problem
we have is that the resin and cells sometimes separate from the thermanox
as the sections come off. The sectioning is not easy, but it can be done
and produces nice results.

Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-isnet.is.wfu.edu *
**************************************************************

On Wed, 9 Aug 1995, Shelly Landon in Spector Lab wrote:

} Hi! I am working with cell monolayers grown on collegen substrates attached
} to thermanox coverslips. The resin is LRW. I mount them so I can cut the
} Z axis. The question is has anyone cut thermanox, if so does this damage
} the knife? The difficulty is in removing the thermanox to get to the cells
} which is easily done with cell monolayers, but not collagen. Any ideas?
} Shelley Landon Kaurin Landon-at-cshl.org
}
}




From: Jay Jerome :      jjerome-at-isnet.is.wfu.edu
Date: Wed, 9 Aug 1995 21:47:49 -0400 (EDT)
Subject: Misc. nonesense

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Just to set the tone for the upcoming MSA meeting, this true story:


A young boy asks the reference librarian who the curly headed little girl
was who is in all the Shirley Temple movies.

The story is worth considering the next time you come up against a
seemingly unsolvable microscope problem.


Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-isnet.is.wfu.edu *
**************************************************************





From: tphillips-at-biosci.mbp.missouri.edu (Tom Phillips)
Date: Wed, 9 Aug 1995 10:17:42 -0600
Subject: Re: PFA and HCl

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Comments: Converted from PROFS to RFC822 format by PUMP V2.2X
Resent-Date: Thu, 10 Aug 95 00:05:49 EDT
Resent-From: Glee Yorke {G0YORK01-at-ULKYVM.LOUISVILLE.EDU}

Anatomical Sciences & Neurobiol.
Phone: 852-5178

----------------------------Original message----------------------------
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I posted a reply earlier and can't remember if I sent it to you directly or
the Microscopy list. If I sent it to you directly, would you mind
forwarding/posting it on the list as I am interested in getting the list
feedback. thanks.

Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility
3 Tucker Hall
University of Missouri
Columbia, MO 65211
(314)-882-4712 (voice)
(314)-882-0123 (fax)






From: Trevor Sewell :      SEWELL-at-uctvms.uct.ac.za
Date: Thu, 10 Aug 1995 10:09:49 +0200
Subject: Re: film scanner

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To Ilene Sugino

I recently received an Ad from Kodak concerning the RFS3570
film scanner it does a variety of film sizes from 35mm at 2100dpi
to 70mm at 800dpi. Kodak also make a thing called PCD Imaging Workstation
which contains a scanner (Kodak Professional Film Scanner 4045)
which does film sizes up to 4x5 with 4kx6k pixels.

You can find out more from their web site:-www.kodak.com

I haven't actually seen either of these machines

Trevor Sewell




From: Caroline Sewry :      csewry-at-rpms.ac.uk
Date: Thu, 10 Aug 1995 09:19:26 -0400 (EDT)
Subject: unsubscribe

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Please unsubscribe - taking a break!





From: Marc Brande :      brande-at-SDSC.EDU
Date: Wed, 9 Aug 1995 11:38:06 -0700 (PDT)
Subject: Digital Imaging of Live Cells

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http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

To: microscopy-at-aaem.amc.anl.gov

I thought that one of the ground rules was no direct commercial advertising
on the listserver.




*****Original message*******************************************************

MolecularCellSpeak List
{molecular-cell-speak-at-mailbase.ac.uk} ,
Microscopy List {microscopy-at-aaem.amc.anl.gov} ,
Info-Bios List {info-bios-at-mom.spie.org} ,
Confocal Microscopy List
{confocal%ubvm.BITNET-at-BITNIC.CREN.NET} ,
Cell Bio List {cellbiol-at-net.bio.net} ,
Biz-Biotech List {biz-biotech-at-netcom.com} ,
Bionews List {bionews-at-net.bio.net} , Biomaterial List
{biomat-l-at-hearn}

Is Your Unique Cell Image Data going Unrecorded?

There is a Solution!

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Monitor and Document Your Live-Cell Experiments Routinely and Frequently.

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To image your live cells, contact:

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Toll-Free: 1-800-645-0848
Local: 619-453-0848
Fax: 619-453-2862
Email: BRANDE-at-SDSC.EDU

Marc C. Brande, M.S. SD3D Email List:3D Imaging
San Diego 3D Imaging Group To subscribe/unsubscribe:listserv-at-bobcat.etsu.edu
3840 Camino Lindo To post a message:sd3d-at-bobcat.etsu.edu
San Diego, CA 92122 Email:BRANDE-at-SDSC.EDU Voice:619-587-4830

****************************************************************************
Dr Ian MacLaren,
IRC in Materials for High Peformance Applications,
The University of Birmingham,
Birmingham B15 2TT,
England




From: Carolyn Emerson :      cemerson-at-kean.ucs.mun.ca
Date: Thu, 10 Aug 1995 09:16:22 -0230
Subject: Zeiss EM 9A specimen holder

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Sender: cemerson-at-KEAN.UCS.MUN.CA

Thanks very much to all who responded to my request regarding obtaining
a used specimen holder for a Zeiss EM 9A TEM. There are a couple on the
way to me. I appreciate the generosity of folk around the microscopy
world - including offers of entire microscopes! Thanks too to Nestor
for his work on this listserver. Carolyn Emerson, Dept. of Biology,
Memorial Univ., St. John's Newfoundland Canada.




From: STEVEN E SLAP :      75767.640-at-compuserve.com
Date: 10 Aug 95 07:56:46 EDT
Subject: Re: Advertising

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Microscopy Listserver {Microscopy-at-aaem.amc.anl.gov}

Dear Ian and colleagues,
As a vendor, I had the same response you did to this particular posting that it
crossed the invisible line between information and solicitation. Several of us
(I am thinking particularly of Chuck Garber) have been very scrupulous about
erring on the side of non-commercialism whenever there was any doubt in our
minds about interpreting this rule. This sometimes involves exercising extreme
restraint. I don't want to pick on this particular vendor, or start another
prolonged harangue, but a little self-discipline goes a long way.
Steven Slap, Vice-President, Energy Beam Sciences





From: dr Keller Eva :      KELEVA-at-igaz.sote.hu
Date: Thu, 10 Aug 1995 15:58:01 +100
Subject: unsubscribe keleva@aaem.amc.anl.gov

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To: microscopy-at-aaem.amc.anl.gov

Sorry for this message. I tried to unsubscribe on the right way, but
nothing has happened.

Please unsubscribe!! keleva-at-igaz.sote.hu




From: tphillips-at-biosci.mbp.missouri.edu (Tom Phillips)
Date: Thu, 10 Aug 1995 09:02:41 -0600
Subject: PF + HCl = carcinogenic gas?

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Message-Id: {v01510101ac4fcf217d99-at-[128.206.15.189]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

D.J. Rawlins in "Light Microscopy" states "...buffers containing chloride
ions (e.g. TrisHCl or phosphate buffered saline) should be avoided as there
is the possibility that HCl vapor could be released which reacts with
formaldehyde to give the very carcinogenic gas bis-chloromethylether."

I have never heard of this before. Is this conventional wisdom? What
about fixes like Karnovsky's that contain CaCl2 to stabilize the membranes?
What do people use to adjust the pH of their fixes? Is this a serious
problem. I have been using HEPES-buffered saline fixes for quite a while.
Any comments would be appreciated.


Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility
3 Tucker Hall
University of Missouri
Columbia, MO 65211
(314)-882-4712 (voice)
(314)-882-0123 (fax)






From: Annette S Kubinec :      akubinec-at-sh.nmfs.gov
Date: Thu, 10 Aug 1995 12:07:35 -0400 (EDT)
Subject: TEM prep room

Contents Retrieved from Microscopy Listserver Archives
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I am a beginner at this topic, so please bear with me.

My lab (government/marine research) is retrofitting a suite
of rooms for a Zeiss TEM 900. I am the safety officer here
and I have some concerns about chemical safety. WE have been advised
that propylene oxide should be stored in an explosion-proof frig.
Does everyone out there do that? Or will a lab-safe flammable
frig suffice? Do they make small versions of the latter?

One more thing for now, must fixing and staining be performed
under a hood, or local ventilation?

Thanks in advance,
Annette Kubinec
NOAA Howard Lab
Ner Jersey
908-872-3011
akubinec-at-sh.nmfs.gov




From: gwe-at-biotech.ufl.edu (Greg Erdos)
Date: Thu, 10 Aug 1995 12:42:41 GMT
Subject: Re: TEM prep room

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} To: Annette S Kubinec {akubinec-at-sh.nmfs.gov}
} From: gwe-at-biotech.ufl.edu (Greg Erdos)
} Subject: Re: TEM prep room
} Cc:
} Bcc:
} X-Attachments:
}
} } I am a beginner at this topic, so please bear with me.
} }
} } My lab (government/marine research) is retrofitting a suite
} } of rooms for a Zeiss TEM 900. I am the safety officer here
} } and I have some concerns about chemical safety. WE have been advised
} } that propylene oxide should be stored in an explosion-proof frig.
} } Does everyone out there do that? Or will a lab-safe flammable
} } frig suffice? Do they make small versions of the latter?
} }
} } One more thing for now, must fixing and staining be performed
} } under a hood, or local ventilation?
} }
} } Thanks in advance,
} } Annette Kubinec
} } NOAA Howard Lab
} } Ner Jersey
} } 908-872-3011
} } akubinec-at-sh.nmfs.gov
} }
} -at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
}
} We have discontinued the use of propylene oxide in our protocols, so we
avoid that problem. Acetone works just as well as a transitional solvent.
}
} Certainly osmium must be used in a fume hood and I would recommend that all
aldehydes be used there as much as possible. Sometimes it is necessary to
do some aldehyde work out side the hood but keep the conainers closed as
much as possible}
}
} It is probably aslo a good idea to use open embedding chemicals in the fume
hood too, again keeping them closed as much as possible when they are
outside the hood.
}
} Staining with aqueous stains can be done safely outside the hood but heavy
metal wastes should be properly disposed of.
}
} There is an Electron Microscopy Safety Handbook by Barber & Moscorro
published by San Francisco Press, Inc., Box 426800, CA 94142-6800.
}
} This would be a good good thing for any safety officers to have if an EM
Lab is part of their domain, and another copy in the lab as well.
}
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
-at- Greg Erdos Phone: 904-392-1295
-at-
-at- Scientific Director,
-at-
-at- ICBR Electron Microscopy Core Lab
-at-
-at- 218 Carr Hall Fax 904-846-0251
-at-
-at- University of Florida E-mail: gwe-at-biotech.ufl.edu
-at-
-at- Gainesville, FL 32611
-at-
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-





From: Richard E. Edelmann :      EDELMARE-at-CASMAIL.MUOHIO.EDU
Date: Thu, 10 Aug 1995 13:26:52 -500
Subject: Re: Advertising

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I disagree with not wishing to make a fuss here, this is truely
blatent advertising, and absolutly should not be tolerated on this
listserver. Let me make clear that I very much enjoy and hartily
encourage vendor participation on this listserver - but the key word
is participation, as in answering or making commentary on specific
issues and questions brought up via the group.

However blatent, unsolicited advertising shall not be tollerated.
I have to deal with these ad's on the news groups enough already.
And we as a group can take the same modes of action as are taken on
the news groups. (1) flame the offenders, (2) contact their
postmaster and clearly state their offense, and (3) if repeated seek
termination of their e-mail access via their postmaster/sysop.

In this case this is not a simple error, the extended list of
CC's shows that this was intended to be a blatent AD. Further more
the list of rules which every subscriber of this group received when
they subscribe clearly state no advestisment.

I apologize to the group for this rant but I truely felt it had
to be said.




From: lesleys-at-pobox.upenn.edu (Lesley S. Smith)
Date: Thu, 10 Aug 1995 14:15:04 -0400 (EDT)
Subject: scopes for sale!

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To anyone interested...

A Zeiss EM 109 and a Zeiss TEM 10 are available. Both are in excellent
shape. The price is negotiable as the seller is motivated! If you are
interested, you can contact Karl at 908-370-8082.

Thank you!!




From: :      MELLIOTT-at-prl.pulmonary.ubc.ca
Date: Thu, 10 Aug 1995 11:25:40 +0800PST
Subject: Sorvall tissuesectionner

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In a recent cleanup of the lab we discovered a Sorvall TC-2 Tissue
Sectionner that hasn't been used in over 10 yrs. Someone in the lab
would like to use it, however, we can't find a manual. Also need to
find a supply of new blades. Who supplies Sorvall equipment now
adays?? Is the Tissue Sectionner still being made. Does someone
have unused blades they no longer require?? Any help would be
greatly appreciated.

Thanks

Mark Elliott
UBC-Pulmonary Research Lab,
Vancouver BC




From: GVKM07A-at-prodigy.com (DR CHARLES A GARBER)
Date: Thu, 10 Aug 1995 15:47:45 EDT
Subject: MSA meeting

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-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --

For list subscribers who will be at the MSA meeting we offer this free
service to make it easier to "stay in touch" when away:

We will have in our exhibit booth a FAX machine during the hours of the
exhibition. Subscribers to the "list" can send or receive FAXed (short)
messages to or from anywhere in the world. Just announce to our booth
staff that you are from the "listserver".

To receive a FAX, give out the following number: 1- (816) 871-3421.
Tell the sender to dial the number and then IMMEDIATELY press the "go"
button so we "see" it as an incoming FAX and not a phone call. Stop by
as often as you like to see if anything has come for you.

We will also be on line much of the time with our web site for those
who do not yet have "web access" and who want to learn how to gain
access. So if the line is busy, be patient and try again.

Yes, we do have an ulterior motive. We would like to meet as many of
our colleagues on the list server as possible. I believe such eye-ball
to eye-ball contact enhances the quality of communications in a world
more and more defined by computer screens and keyboards.

Chuck

======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail:
GVKM07A-at-prodigy.com
West Chester, PA 19381-0656 USA Customer Service:
SpiSupp-at-aol.com

########################################################
WWW: http://mail.cccbi.chester.pa.us/spi/spihome.html
########################################################
======================================================





From: Christine.A.Snay-at-Dartmouth.EDU (Christine A. Snay)
Date: 10 Aug 95 17:09:32 EDT
Subject: immunogold and yeast

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Message-id: {7281455-at-vixen.Dartmouth.EDU}

Dear Colleagues,

Does anyone know of a specfic reference for doing EM immunocytochemistry on
yeast cells? I am trying to localize nuclear pore proteins in yeast using the
whole yeast cell not just a nuclear membrane prep. I have tried preembed IMC
with Fluoronanagold (1.4nm) with silver enhancement, but did not get great
results. Any suggestions would be a great help.

Sincerely,

Christine A. Snay
Dartmouth Medical School
Hanover, NH 03755
e-mail
Christine A. Snay-at-dartmouth.edu
603-650-1905




From: sco.umc2-at-mail.health.ufl.edu
Date: Thu, 10 Aug 1995 13:02:29 -0400
Subject: PFA &HCL

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Message-Id: {s02a4721.054-at-shands.ufl.edu}
X-Mailer: WordPerfect Office 4.0

In our Surg Path Lab, decalcified tissue is routinely washed
before transfer to formalin. We use RDO Rapid Decalcifier which
is concentrated HCl (from Apex Engineering Products Corp).

The container states "simultaneous formaldehyde fixation and
decalcification with RDO should not be performed. A potential
carcinogen could result from this mixture."

The Reactivity Data in the MSDS indicates that hydrogen chloride
and formaldehyde gas react to form bischloromethyl ether, a
carcinogenic compound.

I have always used HCl to adjust the pH of EM fixatives-never
made the connection between Decal(HCl)/formaldehyde
incompatibility and use of HCl when pH'ing EM fixatives.

Can a few drops of dilute HCl in formaldehyde/paraformaldehyde
produce bischloromethyl ether?

Any comments?

Becky Garrison, EM Lab





From: sco.umc2-at-mail.health.ufl.edu
Date: Thu, 10 Aug 1995 13:21:34 -0400
Subject: Oncor address

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Message-Id: {s02a4728.055-at-shands.ufl.edu}
X-Mailer: WordPerfect Office 4.0

Is this the company you want?

Oncor Instruments
Gaithersburg, MD

ph 301-990-0100
fax 301-990-8391







From: Jay Jerome :      jjerome-at-isnet.is.wfu.edu
Date: Thu, 10 Aug 1995 19:58:15 -0400 (EDT)
Subject: Futility of Unsubscription

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Once again you mere mortals have been sending unsubscribe messages-

As scientists you should have figured out by now that unsubscribing
is not allowed. As we have pointed out in the past, once you subscribe you
are on the list for "life" - whichever life comes first, yours or ours.
Furthermore, should you change your e-mail address, we will track you
down and make sure that the list messages get to you.

Have you ever noticed that even if you change phone numbers, the
people who sell aluminum siding, ginzu knives, Kirby vacuum cleaners,
Sears Appliance Service Contracts, and such always seem to find
you no matter what? That is because their computers, like this
listserver computer, are equipped with special software from

Boston American Resource Finders.

BARF software uses proprietary routines to track you down wherever you
are.

The only way to contradict BARF algorithms is to say pretty please and send
your unsubscription request to the correct address:



listserver-at-aaem.amc.anl.gov

with the message:

unsubscribe microscopy yourname-at-your e-mail address



DISCLAIMER: The characters portrayed in this message are not real and
their names were changed to protect the innocent. Which of course begs
the question "Is Nestor real and is he innocent?"




Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-isnet.is.wfu.edu *
**************************************************************





From: STEELE-at-KRDC.INT.ALCAN.CA
Date: Fri, 11 Aug 1995 09:17:56 -0500 (EST)
Subject: Re: Film Scanners

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--Boundary (ID v3WOuY0YgDrNRNY2cptueA)
Content-type: TEXT/PLAIN

As someone who is shopping around for a system with which to collect
digital images from my TEM, I've been reading the recent film scanner
postings with the following questions in mind:

"If you can digitize directly, why would a film scanner be necessary?"
and
"Don't you end up with some HUGE whopping files to store if you are
scanning at that type of resolution?"

My intention is to replace the cost and long turnaround time between
viewing and reporting that I have using standard plates with an "instant",
low cost harcopy solution (likely a CCD / 35mm port interface, thermal
prints at the scope, dry silver for reports). Only diff pats and the
occaisional lattice image would be recorded on film.

The direct digital images I've seen appear to be of sufficient quality for the
majority of standard microstructural images collected here.
So the big question is, do I need a film scanner as well?


--Boundary (ID v3WOuY0YgDrNRNY2cptueA)--




From: gwe-at-biotech.ufl.edu (Greg Erdos)
Date: Fri, 11 Aug 1995 11:15:28 GMT
Subject: Re: immunogold and yeast

Contents Retrieved from Microscopy Listserver Archives
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} Dear Colleagues,
}
} Does anyone know of a specfic reference for doing EM immunocytochemistry on
} yeast cells? I am trying to localize nuclear pore proteins in yeast using the
} whole yeast cell not just a nuclear membrane prep. I have tried preembed IMC
} with Fluoronanagold (1.4nm) with silver enhancement, but did not get great
} results. Any suggestions would be a great help.
}
} Sincerely,
}
} Christine A. Snay
} Dartmouth Medical School
} Hanover, NH 03755
} e-mail
} Christine A. Snay-at-dartmouth.edu
} 603-650-1905
}
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-

You might try Anderson, etal., J. Electron Microsocpy Technique 18:172-175,
1991}

Or

Clark, 1991, Meth. in Enzymology 194: 608-262.
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
-at- Greg Erdos Phone: 904-392-1295
-at-
-at- Scientific Director,
-at-
-at- ICBR Electron Microscopy Core Lab
-at-
-at- 218 Carr Hall Fax 904-846-0251
-at-
-at- University of Florida E-mail: gwe-at-biotech.ufl.edu
-at-
-at- Gainesville, FL 32611
-at-
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-





From: Joyce Craig :      bafpjec-at-uxa.ecn.bgu.edu
Date: Fri, 11 Aug 1995 10:25:44 -0500 (CDT)
Subject: Re: TEM prep room

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The Electron Microscopy Safety Handbook, Vernon C. Barber and Deborah L.
Clayton, San Francisco Press, Box 6800, San Francisco, CA 94101-6800,
ISBN 0-911302-56-5, LC # 85-051796 would be a valuable reference for
you.
I do all staining, fixation, and embedding under a hood, and have my
embedding ovens under a hood. Unopened bottles of propylene oxide are
kept in the refrigerator and opened bottles under the hood. Before I
came to this lab, an explosion had occurred in the refrigerator as an
opened bottle of prop ox had been in the refrigerator, fumes had
accumulated, and evidently explosion occurred.
I worked in n lab without air conditioning at one time and all the lids
popped off the vials.
On Thu, 10 Aug 1995,
Annette S Kubinec wrote:

} I am a beginner at this topic, so please bear with me.
}
} My lab (government/marine research) is retrofitting a suite
} of rooms for a Zeiss TEM 900. I am the safety officer here
} and I have some concerns about chemical safety. WE have been advised
} that propylene oxide should be stored in an explosion-proof frig.
} Does everyone out there do that? Or will a lab-safe flammable
} frig suffice? Do they make small versions of the latter?
}
} One more thing for now, must fixing and staining be performed
} under a hood, or local ventilation?
}
} Thanks in advance,
} Annette Kubinec
} NOAA Howard Lab
} Ner Jersey
} 908-872-3011
} akubinec-at-sh.nmfs.gov
}




From: germ-at-eastman.com (Lou Germinario)
Date: Fri, 11 Aug 1995 08:46:37 -0500
Subject: unsubscribe

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Message-Id: {199508111010.MAA09252-at-gate.sbbio.be}

Please unsubscribe

Dr. Louis T. Germinario
Eastman Chemical Company
P. O. Box 1972
Kingsport, TN 37660-5150
(615) 229-4047
(615) 229-4558 fax
germ-at-eastman.com






From: germ-at-eastman.com (Lou Germinario)
Date: Fri, 11 Aug 1995 08:46:39 -0500
Subject: unsubscribe

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Message-Id: {199508111240.AA24007-at-eastman.com}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Please unsubscribe.

Dr. Louis T. Germinario
Eastman Chemical Company
P. O. Box 1972
Kingsport, TN 37660-5150
(615) 229-4047
(615) 229-4558 fax
germ-at-eastman.com






From: EMLAB-at-opus.mco.edu
Date: Fri, 11 Aug 1995 09:09:46 -0500 (EST)
Subject: Re: TEM prep room

Contents Retrieved from Microscopy Listserver Archives
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Dear Annette,

Here at MCO we do have safety in mind, all of our tissue processing is
performed in a fume hood. The resin (Spurr's) is polymerized in an oven which
is vented to an exhust fan. All of our Flammable Chemicals are in a flammable
cabinet which is also vented to an exhust fan. Our refrig is a type which
was made for storage of flammable materials. The best advice is not to have
a lot of flammable materials in the lab, this means good inventory/ordering on
your part.
A few other safety items: Have in the lab at readily accessable points
the following; fire extinguisher, first aid kit, chemical spill kits, eye wash
stations,(shower), soap and towels, biohazard bags, sharps containers,lots of
warning signs, MSDS's, and the list goes on and on.
The "safer" you can make a lab the begin with the better. One other
item, pay particular attention to the work flow/space. You do not want areas
where people will be bumping into each other - SPACE - is the key word here.

Best of Luck,

Ed Calomeni
Medical College of Ohio
Toledo, OH
emlab-at-opus.mco.edu




From: STEVEN E SLAP :      75767.640-at-compuserve.com
Date: 11 Aug 95 07:58:19 EDT
Subject: Re: Sorvall tissue sectioner

Contents Retrieved from Microscopy Listserver Archives
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The best source for parts and advice regarding the old Sorvall tissue chopper is
a former Sorvall sales/service expert named Bill McGee. Bill's company is
called Microtome Services Co, 7568 Florian Way, Liverpool, NY 13088. His phone
number is (315)451-1404 (no fax or e-mail, sorry). Tell him Steve sent you.
Steven Slap





From: sco.umc2-at-mail.health.ufl.edu
Date: Fri, 11 Aug 1995 04:39:26 -0400
Subject: digital cameras

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Message-Id: {s02b1835.076-at-shands.ufl.edu}
X-Mailer: WordPerfect Office 4.0

I have two digital cameras:

one is a Kodak the other a JVC

the Kodak is 1000 x 1000 lines of resolution greyscale.
we use it for electron microscopy, and it is adequate. The JVC
will capture up to 3500 x 3000 lines, color 16 million.
The JVC requires an immobile subject. we use it for light
microscopy, and the screen images are astounding.

There are many advantages to video photography, but, before you
buy, consider:
price
image storage (jpg format may not be acceptable for legal
purposes)
printing

I am writing an article on this subject, and I actually built the
JVC system from parts. If you know of a journal which would be
interested in publishing my findings, please tell me.

I welcome questions
Scott Hollington MD





From: bozzola-at-siu.edu (John. J. Bozzola)
Date: Fri, 11 Aug 1995 17:27:30 -0600
Subject: EM: Virus Images

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Microscopists/Virologists:

I am looking for a plate or plates that show electron micrographs (sections
and neg stains) of the major virus families. G.D. Hsiung published such a
plate several years ago but I can not locate her. Does anyone have such
information or know where I could locate Dr. Hsiung (e-mail, phone, etc).

Also, anyone know where to locate actual micrographic prints (not digital
images) of alpha- flaviviruses, Ebola, etc. We would like to include them
in a textbook we are revising.

Thank you all very much.

Peace.


#############################################################################
John J. Bozzola, Director
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
#############################################################################






From: ldm2-at-apollo.numis.nwu.edu (L.D.Marks)
Date: Sat, 12 Aug 1995 12:09:55 -0500
Subject: Teaching using the Web ?

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I am going to be teaching two courses next year:
a) Introduction to Materials Science
b) Introduction to Electron Microscopy

I am thinking about using Web Pages for various material, at the
most obvious level homework (and answers). Has anyone had experience
doing this in the past, any suggestions (or horror stories), and is
there anyone who has material which might be useful ?




From: spignole-at-ix.netcom.com (Susanne Brandom)
Date: Sat, 12 Aug 1995 13:35:14 -0700
Subject: MicroWorld Resources and News WWW Site

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To all,

MicroWorld Resources and News© is pleased to announce the opening of
a new World Wide Web site dedicated to microscopy and microanalysis.
The site is a resource center for scientists, educators, and
technicians that are exploring the Internet. It includes an extensive
guide to the Internet that will help locate information on techniques,
equipment and applications, a newsletter with monthly features, a
library with references, book reviews and an extended glossary, and a
Products and Services Directory.

As a part of our opening at MSA'95, news from the meeting will be
posted in our What's New column. If you would like to participate,
please stop by booth 464 and we will post the information on the
Internet during the show. If you can't make the show, I will be
checking for FAXES at the SPI booth.

Susanne Pignolet Brandom, Ph.D.
spb-at-wwa.com or spignole-at-ix.netcom.com
708-548-6522

MicroWorld Resources and News
http://mwrn.ms.wwa.com/topfile.htm








From: dcg-at-ariadne.phys.uts.edu.au (David Green)
Date: Sun, 13 Aug 1995 11:47:10 -1200 (EDT)
Subject: Re: Teaching using the Web ?

Contents Retrieved from Microscopy Listserver Archives
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gbs-at-ariadne.phys.uts.edu.au (Geoff Smith),
Microscopy-at-aaem.amc.anl.gov (Microscopy List)
In-Reply-To: {199508121709.AA04206-at-apollo.numis.nwu.edu} from "L.D.Marks" at Aug 12, 95 12:09:55 pm
X-Mailer: ELM [version 2.4 PL23]
Mime-Version: 1.0
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Content-Transfer-Encoding: 7bit
Content-Length: 2607

L.D.Marks posted an enquiry about the use of the WEB for teaching.
There are some general issues of "teachnology" which may be of general interest so
I have posted this response to the list.(apologies to those who have "research only" jobs!)


I have been developing mixed feelings about the extent to which the Web
SHOULD be used as a teaching tool:
* A complete replacement for the human being it should never be.
* The electronic library character (+speed/convenience) is a bonus for students
* However, to make a teaching tool most beneficial it needs to be genuinely designed for
student learning, not just messing about and picking up info here & there.

I use a Subject homepage as a electronic bulletin board and summary sheet for my students.

I am currently developing an interactive "webbed" version of a computer simulation tool
for understanding magnetic properties of materials.
(unfortunately I don't get to teach much microscopy at the moment)

Perhaps something else to consider is the use of an email newsgroup for students,
it keeps them in contact with you and each other. (not unlike this Listserver !!)

I'd welcome feedback/discussion on this issue.


} I am going to be teaching two courses next year:
} a) Introduction to Materials Science
} b) Introduction to Electron Microscopy
} I am thinking about using Web Pages for various material, at the
} most obvious level homework (and answers). Has anyone had experience
} doing this in the past, any suggestions (or horror stories), and is
} there anyone who has material which might be useful ?

I think what Dr Marks is proposing is about right and not too difficult to achieve
provided:
* your students are ALL computer and net confident and have easy access to the WWW
* you are happy for your material to be available to "the world" (there are ways around this)
* you build in good feedback into the electronic homework sheets so students aren't just left
knowing they're are wrong



PS: Who's coming to beautiful Sydney next February ?????




Kind regards,

Dr David Green
Lecturer in Applied Physics
University of Technology, Sydney
P.O. Box 123 TELEPHONE: + 61 2 330 2203
Broadway 2007 NSW A MESSAGE: + 61 2 330 2206
Australia FACSIMILE: + 61 2 330 2219
EMAIL: dcg-at-phys.uts.edu.au
WEB: http://www.phys.uts.edu.au/~dcg/DavidGreen.html
or http://www.uts.edu.au/ if you like "surfing"
------------------------------------------------------------------
Watch out on the information superhighway...
crazy driver with "P" plates.
------------------------------------------------------------------





From: Nina S Allen :      nallen-at-unity.ncsu.edu
Date: Sun, 13 Aug 1995 18:33:14 -0400 (EDT)
Subject: Re: V150 Address for Oncor Instruments

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Oncor Imaging is
200 Perry Parkway, Suite 1
GAithersburg, MD 20877
1 -800-237-7706

Nina Allen




From: editor-at-microscopy-online.com (Editor)
Date: Sun, 13 Aug 1995 16:52:10 -0700
Subject: Commercial Use of This List Server

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X-Sender: mail-at-microscopy-online.com
Message-Id: {v01520d04ac5432e13179-at-[165.247.1.58]}
Mime-Version: 1.0
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The recent exchange of messages regarding what is acceptable for commercial
vendor postings on this list server has prompted me to remind you that
Microscopy Online offers a forum for posting commercial information that
non-commercial users will find entirely acceptable. Microscopy Online is a
server on the WWW that allows everyone (commercial or not) access to post
information (text, graphics) that is related to microscopy, whether you
have access to the WWW or not. The URL is

http://www.microscopy-online.com/


Editor
Microscopy Online - http://www.microscopy-online.com/
editor-at-microscopy-online.com






From: m.dickson-at-unsw.edu.au (melvyn dickson)
Date: Mon, 14 Aug 1995 11:18:39
Subject: Re: use of propylene oxide

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To: microscopy-at-aaem.amc.anl.gov

I forbade the use of propylene oxide in my lab 25 years ago. It is too much
of a fire/explosion risk. Look at the flashpoint temperature on the bottle!
minus 29 deg. celsius! Even petrol is better at minus 18 deg. C.

Luft suggested its use as a transitional solvent because he argued the epoxy
group meant any solvent carried over into the embedment would be incorporated
into the molecular structure of the epoxy resin. But the boiling point is 35
deg. C. As soon as an embedment is put into a curing oven at 60 deg. any
epoxy propane in the resin will flash into vapour and lucky it doens't blow
the door off! I conclude the only benefit of epoxy propane is its
miscibility and low boiling point. We use acetone, which boils around 55 deg.
It is only about as dangerous as petrol and will vaporise easily at 60 deg. in
the curing oven. Ethanol, boiling at 78 deg. will not, and resin containing
carried over ethanol stays sticky.




From: m.dickson-at-unsw.edu.au (melvyn dickson)
Date: Mon, 14 Aug 1995 11:32:43
Subject: Re: Film Scanners

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To: microscopy-at-aaem.amc.anl.gov

In article STEELE-at-KRDC.INT.ALCAN.CA writes:


} "If you can digitize directly, why would a film scanner be necessary?"

Once you start working with digital files you won't want to go back to your
darkroom. THEN you need some way to digitise the plates/films you have
archived over the years.

} and
} "Don't you end up with some HUGE whopping files to store if you are
} scanning at that type of resolution?"

Scan at a resolution thats acceptable. Say 1024x1024. You can store 500 such
images on one CD-ROM at a cost of $18 per disc. Films are about $2 a shot
including processing.




From: Prof. P.J. Goodhew :      goodhew-at-liverpool.ac.uk
Date: Mon, 14 Aug 1995 09:22:43 +0100 (BST)
Subject: unsubscribe

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Please unsubscribe - I am on holiday




From: Software department :      software-at-oimag.win-uk.net
Date: Mon, 14 Aug 1995 10:20:31
Subject: Re: EDS Detector HV Stability

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X-Mailer: WinNET Mail, v2.30
Message-ID: {776-at-oimag.win-uk.net}
Reply-To: Software department {software-at-oimag.win-uk.net}
To: USERHHXS-at-um.cc.umich.edu, microscopy-at-aaem.amc.anl.gov

Obviously, the effect depends on the system you have and the
precision with which you want to acquire spectra.

When power is applied to the detector, there are various time
constants, both electronic and thermal, which affect how long the
system takes to reach a stable operating point. Most electronic
circuits generate heat and gain and offsets are temperature
sensitive which is why the thermal time constant is relevant. If
the detecting crystal has been exposed to a high radiation dose,
removing and restoring the HT can affect the behaviour, but there
are no rules and not all systems perform the same way.

An easy way to check this is to record a series of spectra
sequentially, immediately after the system is switched on from cold.
Whereas gross changes will be apparent in the first few minutes,
the spectra will probably seem to settle down after half an hour
typically. However, if you want to do accurate spectrum processing
and expect the software to sort out severe peak overlaps, accurate
peak position and width is essential and you will need a more
sensitive method to pick up the changes.

If you don't have any peak fitting diagnostic software to hand,
one thing you can do is simply subtract one spectrum from the next
one acquired and view the difference spectrum. Provided the two
spectra have reasonable statistics, the difference spectrum will
show up minute differences in gain, offset or resolution, in any
region where there is a major peak. A shift of only 1eV between
spectra will typically produce a bipolar residual of very roughly
1% of the peak height. Of course, you will have to make sure your
microscope has good beam current stability to do these tests. If
not, you will have to normalise each spectrum to the same area
before subtraction.

Peter Statham
Oxford Instruments Microanalysis Group

} I am interested in opinions concerning the relative merits
} of keeping an EDS HV bias on continiously, as opposed to
} shutting it off after every use. In other words, is there
} any advantage wrt to stability of the electronics or
} energy calibration to keeping it on?
}

-----------------------------------------------------------------
Please reply to this e-mail with the name of the person you
wish to receive it on the subject line
(e.g. "FAO Janet Smythe/..subject.."),
as this is a shared e-mail address.







From: XiaoGuang Ning :      ningx-at-mcmail.CIS.McMaster.CA
Date: Mon, 14 Aug 1995 10:55:35 -0400 (EDT)
Subject: Answer to the Loss of Information on Negatives

Contents Retrieved from Microscopy Listserver Archives
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Dear Microscopists:

Several days ago I put one message about the loss of information (MAG. &
scale-bar) on negatives in JEOL 2010F microscope. Thanks to all the
people who gave me kind correspondences.

Nowadays, we know why we lost the information. It is because we use the
FLC, i.e. free lens control, function. Even though I only changed the
current of the condenser lens and did not change the magnification, the
CRT under FLC will in no way give you information about MAG. on negatives.
That is not the problem of the microscope, but what we should learn. It
is my pleasure to post the "answer" here for your interest.

Best Regards

X.G.NING




From: wise-at-vaxa.cis.uwosh.edu
Date: Mon, 14 Aug 1995 10:33:33 +0000
Subject: Storage devices

Contents Retrieved from Microscopy Listserver Archives
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To all,

I have seen discussion of several of the various storage formats
for archiving electronic images--i.e. read/write CD, magneto optical,
tapes, etc. What are the collective thoughts on 3.5" cartridges (such as
Syquest)? They can hold up to 270 Mb and would seem to be a cheap (~$65)
alternative that can be read by any machine with a 3.5" drive.

Bob


Robert R. Wise, PhD
Director, UWO Electron Microscope Facility
Department of Biology
University of Wisconsin Oshkosh
Oshkosh, WI 54901
(414) 424-3404
(414) 424-1101 fax
wise-at-vaxa.cis.uwosh.edu






From: Kimberly (Wilson) Russell :      kwilson-at-admin.ogi.edu
Date: Mon, 14 Aug 1995 08:57:58 -0700 (PDT)
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
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unsubscribe





From: Michael Cammer :      cammer-at-aecom.yu.edu
Date: Mon, 14 Aug 1995 12:03:25 -0400 (EDT)
Subject: Re: Storage devices

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Less for archiving and more for convenience sake, although this works for
archiving, we have 230 MB read/write magneto optical disk drives on each
of our Macintoshes and lots of other people around the school have them
too (either Olympus or Fujitsu mechanism). The upsides are that the disks
are cheap ($40), small, hold a lot, are random access, each user is
responsible for his/her own data and having a drive on each machine
eliminates the need for any type of network support. The downside is
that they are slower than some other media choices. For instance, for
moving large amounts of data from hard disk to archive and back, a 4mm
DAT drive is faster, but attempting to use this in a multi-user faciility
just didn't work.
-Michael Cammer

On Mon, 14 Aug 1995 wise-at-vaxa.cis.uwosh.edu wrote:

} To all,
}
} I have seen discussion of several of the various storage formats
} for archiving electronic images--i.e. read/write CD, magneto optical,
} tapes, etc. What are the collective thoughts on 3.5" cartridges (such as
} Syquest)? They can hold up to 270 Mb and would seem to be a cheap (~$65)
} alternative that can be read by any machine with a 3.5" drive.
}
} Bob
}
}
} Robert R. Wise, PhD
} Director, UWO Electron Microscope Facility
} Department of Biology
} University of Wisconsin Oshkosh
} Oshkosh, WI 54901
} (414) 424-3404
} (414) 424-1101 fax
} wise-at-vaxa.cis.uwosh.edu
}
}
}




From: bdgranby-at-students.wisc.edu (dry cleaner)
Date: Sun, 13 Aug 1995 21:26:04 +0000
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
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Message-Id: {199508150231.VAA42873-at-audumla.students.wisc.edu}

Please unsubscribe.




$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$
$$$$$$$$$$$$$$$$$$$$$$$$$$$ $$$$$$$ from laughter or from fright.$$$
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$$$$$$$$$$$$$$$$$$$$ would gag upon your sight, $$$$$$$$$$$$$$$$$
$ ---Residents $$$$$$$$$$$$$$$$$
$$$$$$$$$$$$$$$$$$





From: Tadakatsu OHKUBO :      ohkubo-at-sanken.osaka-u.ac.jp
Date: Tue, 15 Aug 1995 12:11:37 +0900
Subject: unsubscribe

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unsubscribe microscopy





From: Self :      BIOLOGY/WESLEYSM
Date: Fri, 11 Aug 1995 10:16:29
Subject: Replies to cacodylate question

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Comments: Authenticated sender is {wesleysm-at-biology.und.ac.za}

------- Forwarded Message Follows -------

Dear colleagues
Thanks to those of you who came forward with comments regarding
the merits of using cacodylate buffer in specimen preparation for
microscopy.

Replies could be summarised as follows:
1. The use of cacodylate buffer is recommended for enzyme
cytochemistry work for the following reasons:
a. The use of Sorensens phosphate buffer can be damaging to
mitochondrial activity. b. The use of phosphate buffers is
incompatible with lead precipitation reactions. c. Cacodylate buffers
will not react with aldehyde fixatives, as other amine-containing
buffers will (e.g. TRIS).

2. It seems that the use of cacodylate by 'tradition' or because
users follow " a paper I read some years ago..." is not unique to
this lab. Common sense dictates for a pilot study to be done at the
beginning of a project to assess whether the results from using this
buffer are, in fact, superior to alternative ones. Unfortunately,
this is usually ignored in the interset of expediency, and the
"proven" method wins.

3. Perhaps the most surprising piece of information was that
cacodylate buffer CAN support microorganisms, as is the case with
demethylating bacteria. (Another theory bites the dust!).

Everyone agrees on its toxicity, the need to use it for specialised
applications only, and to collect residues for its disposal. (We
collect it in a 2.5l bottle in the fume-hood, and it is removed by a
waste disposal firm. Does anyone known what they do with it?)

This has been an interesting exercise for me, and one which makes
this medium an invaluable forum to exchange ideas.


Thank you.


James Wesley-Smith
Electron Microscope Unit
George Campbell Building
University of Natal
Durban, South Africa





From: Fred Hayes :      fahayes-at-ucdavis.edu
Date: Tue, 15 Aug 1995 05:29:55 -0700 (PDT)
Subject: Spurr's resin formula

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Does anyone out there have a Spurr's formula measured by volume instead
of weight. I need the ml/g equilavent.

Can this resin be polymerized by microwave?

Many thanks!


Fred A. Hayes
Dixon, CA
916-678-6280





From: Martin Newman-1 :      Martin_Newman-1%notes-at-sb.com
Date: 15 Aug 95 14:30:47 EDT
Subject: TEM - Grid stainers

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Message-Id: {9508151638.AA4381-at-pho018.sb.com}
To: Microscopy {Microscopy-at-aaem.amc.anl.gov}

Does anyone know of any reliable TEM grid stainers. We have a BIORAD polaron
E9500 stainer which constantly blocks up and is unreliable.
Ta.





From: Rebecca L. Buerkett :      rbuerket-at-moose.uvm.edu
Date: Tue, 15 Aug 1995 12:47:15 -0400 (EDT)
Subject: subscribe

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subscribe
rbuerket-at-moose.uvm.edu




From: Stephen_Grayson-at-mailstop.pharmetr.com
Date: Tue, 15 Aug 1995 07:02:20 PDT
Subject: EM sale

Contents Retrieved from Microscopy Listserver Archives
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Message-ID: {1995Aug15.070220.254693-at-mailstop.pharmetr.com}
To: microscopy-at-aaem.amc.anl.gov

MUST SELL

A Zeiss 10CA transmission electron microscope in top condition with Coolwell
closed-loop cooling system, recently overhauled rotary and diffusion pumps,
two 3 1/4 x 4" negative cassette carriers with 90 hegative cassettes. The
microscope maintains an excellent vacuum, a point to point resolution of 3
angstroms, and accelerating voltages of 20-40-60-80- and 100kV. The
microscope comes with all of the necessary accessories, including extra
filaments. The asking price is $35,000, however the best offer will be
accepted.

Also available: a complete darkroom setup, virtually brand new, including
fully equipped stainless steel sink (24x72x5"), developing tanks and water
jacket processor, print washer, RC print dryer, negative drying oven, Durst
L1200 enlarger with condensors, lens and carrier, and sodium arc safelight.
In addition: a Leica cryocut 1800 cryostat, a Sorvall MT-2B ultramicrotome,
and LKB 7800 glass knifebreaker. All of these items may be sold as a package
or separately. Please E-mail or call for more information and pricing:
Steve Grayson: Tel# (415) 617-9327 or FAX (415) 853-6301
***************************************************************************
Pharmetrix, 1330 O'Brien Drive, Menlo Park, CA, 94025
Main: (415)688-0100 Fax: (415)688-0115
The views expressed in this posting those of the individual author only.
***************************************************************************




From: wise-at-VAXA.CIS.UWOSH.EDU
Date: Tue, 15 Aug 1995 17:14:09 +0000
Subject: LKB 7800 Knifemaker

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To all,

Does anyone out there have the secret to consistently producing
good glass knives on an LKB 7800 knifemaker? I have cleaned, adjusted, and
fiddled with it for two years and can get still get only average knives,
and those only some of the time. Students new to the instrument almost
never get good results. I contacted LKB and they sent a couple of
brochures that seemed to be only marginally helpful. Is it in the black
magic associated with cleaning the glass, brand of glass (we use Ted Pella
glass), or the phase of the moon?

Bob


Robert R. Wise, PhD
Director, UWO Electron Microscope Facility
Department of Biology
University of Wisconsin Oshkosh
Oshkosh, WI 54901
(414) 424-3404
(414) 424-1101 fax
wise-at-vaxa.cis.uwosh.edu






From: Gerry LITTLE :      ANGJL-at-medicine.newcastle.edu.au
Date: Wed, 16 Aug 1995 10:13:14 GMT +11
Subject: Re: TEM - Grid stainers

Contents Retrieved from Microscopy Listserver Archives
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G'day Martin,

Depending on the number of grids you stain per day, this little
instrument might be of use to you. It is the Hiraoka Staining Dish
which will stain up to 40 grids at one time. We purchased ours
through Probing & Structure (Townsville, Qld. Australia) or you can
get it through Electron Microscopy Sciences. I use it all the time
and find it very reliable and easy to use.

Regards,
Gerald.

Dr. Gerald J. Little,
The Neuroscience Group,
Discipline of Anatomy,
Faculty of Medicine and Health Sciences,
The University of Newcastle,
Callaghan, New South Wales,
Australia, 2308.
Ph. (61 49) 21 5618
Fax (61 49) 21 6903
Email ANGJL-at-Medicine.Newcastle.edu.au





From: D.Wild-at-mirinz.org.nz
Date: Wed, 16 Aug 1995 13:39 +1200
Subject: glass knives

Contents Retrieved from Microscopy Listserver Archives
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I believe our lab. once tried another brand of glass after always
using LKB glass and quickly returned to the more expensive LKB brand.
However that was quite a few years ago, we brought enough glass to
last for ever...!




From: SALLY STOWE :      STOWE-at-rsbs-central.anu.edu.au
Date: Wed, 16 Aug 1995 11:59:51 EST10
Subject: Digital Image Restoration Microscopy

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Digital Image Restoration Microscopy, or Deconvolution Microscopy...
I am looking for any information, including contact email or fax nos
of the manufacturers.
Thanks,
Sally .
----------------------------------------------------------------------
Sally Stowe Australian National Univ.
Facility Coordinator Canberra, AUSTRALIA
ANU Electron Microscopy Unit Ph 61 6 249 2743
RSBS, Box 475
Email stowe-at-rsbs-central.anu.edu.au FAX 61 6 249 4891
------------------------------------- --------------------------------
-





From: Tony Bruton :      BRUTON-at-gate2.cc.unp.ac.za
Date: Wed, 16 Aug 1995 8:36:11 +200
Subject: LKB 7800 Knifemaker

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To: MICROSCOPY-at-AAEM.AMC.ANL.GOV

Bob Wise, University of Wisconsin seems to be following a track that most
owners of LKB 7800's seem to have at one time or another. But there is a
solution at hand Bob. First let me reassure you;

1) Phase of the moon is important, and,
2) Not all microtomy glass is equal

We are fortunate to have a gifted engineer in one of our workshops and he has
'breathed' on our 7800 to remarkable effect. Our target was between 6 and 8 out
of 10 good knives. Jan Slot, when he visited our laboratory, said that he had
never met anyone who had investigated the working of a knifemaker as
thoroughly. But enough waffle - we published the mods;

Journal of Microscopy, Vol.168, Pt 1, October 1992, pp111-114.
'Further modification of the LKB 7800 series Knifemaker for improved
reproducibility in breaking 'cryo' knives'

Stay in touch , there have been further refinements - but modify your unit
according to the detailed instructions given in the paper first.

Tony Bruton
Electron Microscope Unit
University of Natal
Pietermaritzburg
Kwazulu Natal;
South Africa
Tel: (0331) 2605155, Fax : (0331)2605776,
email : BRUTON-at-EMU.UNP.AC.ZA




From: Trevor Sewell :      SEWELL-at-uctvms.uct.ac.za
Date: Wed, 16 Aug 1995 11:41:22 +0200
Subject: Training Videos

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Dear All,

We would like to build up a library of training videos on aspects of
microscopy. Does anyone know any good ones? I would also appreciate
information on their current availability.

Many Thanks

Trevor Sewell
EM Unit
University of Cape Town





From: Tony Bruton :      BRUTON-at-gate2.cc.unp.ac.za
Date: Wed, 16 Aug 1995 12:24:49 +200
Subject: LKB 7800 Knifemaker

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To: MICROSCOPY-at-AAEM.AMC.ANL.GOV

Bob Wise, University of Wisconsin seems to be following a track that most owners of LKB 7800's seem to have at one time or another. But there is a solution
at hand Bob. First let me reassure you;

1) Phase of the moon is important, and,
2) Not all microtomy glass is equal

We are fortunate to have a gifted engineer in one of our workshops and he has 'breathed' on our 7800 to remarkable effect. Our target was between 6 and 8
out of 10 good knives. Jan Slot, when he visited our laboratory, said that he had never met anyone who had investigated the working of a knifemaker as
thoroughly. But enough waffle - we published the mods;

Journal of Microscopy, Vol.168, Pt 1, October 1992, pp111-114.
'Further modification of the LKB 7800 series Knifemaker for improved reproducibility in breaking 'cryo' knives'

Stay in touch , there have been further refinements - but modify your unit according to the detailed instructions given in the paper first.

Tony Bruton
Electron Microscope Unit
University of Natal
Pietermaritzburg
Kwazulu Natal;
South Africa
Tel: (0331) 2605155, Fax : (0331)2605776,
email : BRUTON-at-EMU.UNP.AC.ZA




From: kennedy-at-nsi.edu (grace kennedy)
Date: Wed, 16 Aug 1995 08:14:10 -0800
Subject: LKB 7800

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The old LKB glass is available from Ted Pella, and it really makes a
difference. Has your knifemaker had a good tuneup lately (scoring wheel,
clean slides of the head, caliper-measurement of the squares, fresh
dampening pad)?? Careful attention to all of the above can make a huge
difference in knife quality. I recently put in a nice fresh scoring wheel
and carefully tweaked the scoring pressure with a great increase in knife
yield (and consistency). Grace Kennedy PS Caliper-measure your squares!!






From: Ronald LHerault :      lherault-at-acs.bu.edu
Date: Wed, 16 Aug 1995 12:20:32 -0400 (EDT)
Subject: Millonig's Buffer

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Would someone please e-mail me the protocol for mixing up Millonig's
phosphate buffer (I believe it was developed in 1964).

Thanks,

Ron
lherault-at-acs.bu.edu




From: haavisto-at-students.wisc.edu (Amy Jo Haavisto)
Date: Wed, 16 Aug 1995 11:49:05 -0500
Subject: Course Announcement: South African Workshop

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Introducing the workshop:
IMAGING CELLULAR DYNAMICS DURING DEVELOPMENT & REPRODUCTION
and its accompanying symposium:
EX OVO OMNIA: FROM THE EGG, EVERYTHING!


Dates: Workshop - January 7-24, 1996
Symposium - January 21-24, 1996

Place: University of the Witwatersrand
Johannesburg, South Africa

The workshop course is designed to familiarize young scientists
with modern approaches for exploring structural events at the cell and
molecular level. The course will rely heavily on invertebrate and
vertebrate eggs and embryos to explore dynamic events in the cell cycle and
during development. Participants will use advanced imaging technologies
through full time laboratory work, and be exposed to advanced topics in
Cell Biology, Developmental Biology, and Reproductive Biology during daily
lectures. Twenty-four young scientists will be accepted, with a preference
for doctoral level (or equivalent) back-ground, who are in the beginning of
their scientific careers.

To apply for the workshop, please mail, fax or e-mail no later than
September 15:
1. Name, address, fax, email, and academic affiliation. 2. Curriculum
vita. 3. Three letters of recommendation. 4. A proposal which states:
(A) the reason for applying. (B) how the course will benefit you and your
local community both in terms of scientific research, as well as scientific
instruction. (C) proof of complementary financial support from your
institution, as well as anticipated travel expenses.

To: Professor Barry Fabian Professor Gerald Schatten
Univ. Of the Witwatersrand Univ. Of Wisconsin
Private Bag 3, WITS 1117 W. Johnson
Johannesburg, 2050 S. Africa Madison, WI 53706 USA
Fax: 27.11.339.3407 Fax: 608.262.7319
Email: Barry-at-gecko.biol.wits.ac.za Email:Schatten-at-macc.wisc.edu

A special International Symposium on Developmental Biology has been
arranged to coincide with the conclusion of the laboratory training course.
The theme of the symposium addresses development at the molecular, cellular
and organismic levels, with presentations in the form of talks and posters.
Contact Barry Fabian or Gerald Schatten for information about poster
presentation and registration.

Amy Haavisto
Schatten Lab
University of Wisconsin - Madison
Haavisto-at-students.wisc.edu
Tel: 608-262-2048
Fax: 608-262-7319






From: dlb-at-aruba.ccit.arizona.edu (David Bentley)
Date: Wed, 16 Aug 1995 10:30:58 -0700
Subject: Re: Millonig's Buffer

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I found the Preparation for Millonig's buffer in Hayat's Electron
Microscopy. Surprise, it isn't in Manual of Electron Microscopy by
G.Millonig (1974).

The preparation is
Millonig's Buffer (1964)(Taken from Hayat, M.A., 1989, Electron
Microscopy, 3rd ed., pg 23)

NaH2PO4.H2O 1.8 gm
Na2HPO4.7H2O 23.25 gm
NaCl 5.0 gm
H2O, glass distilled (to make) 1000 ml

pH is 7.4 osmolarity is 440 mosm


I'm sorry the subscripts didn't transfer

} Would someone please e-mail me the protocol for mixing up Millonig's
} phosphate buffer (I believe it was developed in 1964).
}
} Thanks,
}
} Ron
} lherault-at-acs.bu.edu
}
later
dlb





From: wise-at-VAXA.CIS.UWOSH.EDU
Date: Wed, 16 Aug 1995 12:58:35 +0000
Subject: Making glass knives

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To all,

Thanks for all the response to my query on LKB 7800 knife makers.
I got plenty of tips to try and sources to look into. I share the
accumulated wisdom with the net (even though some of it you have already
seen).
I have three weeks before the semester and my EM techniques class
start to get our KnifeMaker tuned up. I'll let you know how it performs in
the hands of the ulitmate consumers (students).

Bob

****************

Comments ( in no particular order):

---For resin embedded sections, the solution might be to make a softer
resin mix and cut thicker sections. ---If you want really sharp glass
knives for cutting cryosections then check out J. Microsc. (1993 169:85-88)
where I describe a simple way to get very good glass knives with only
minimal modification.
---Another way to increase your chances of getting a good edge is to use 10
mm thick glass, which is hard to break but even really bad glass knives
have a good length of useable knife edge in the middle.
---We have had problems with glass from other suppliers so stick to the
strips made in Sweden (we get ours from EMS).
---The best knifemakers were the really old ones with a light blue case.
The engineering on them is so much better than on the new ones.
---Try using LKB glass. It is slightly different in thickness than any
other glass manufactured.
---Could you define what a good knife is (% of usable defect-free edge)? I
consider 25-30% good edge normal and 50% a lucky break. It is not at all
unusual to have half the knives made rejected upon inspection.
---Perhaps the scoring wheel is worn. Remove it and inspect under a
dissecting microscope. Look at the cutting edge; if the surface looks like
an upside-down "V" (/\) it's OK. If that apex has a flat rim /-\ then
the score may not be enough to define the break initiation zone.
---Pella glass is fine, if you can find some LKB glass it would be worth
trying.
---If you need a scoring wheel, try Leica. They absorbed LKB, Cambridge,
A/O and Reichert recently.
--- A slow tensioning of the pressure is the recommended procedure but you
probably already knew that.
---Your problem might be the resin hardness. We never managed to cut
sections using the resin formula we use with diamond knives, it only worked
with a softer resin and thicker sections.
---You might also check out Stang 1988 J. Microsc. 149:77-79 for a really
serious modification.
---Another interesting paper is Roberts 1975 J. Microsc.103: 113-119. This
is a description of how tungsten coating glass knives can inprove their
sectioning properties and shelf life. However, I can tell you that this
does not improve a poor quality knife and too much tungsten can ruin a good
knife.
---There is a short chapter in "Griffiths, G. 1993. Fine Structure
Immunocytochemistry. Springer Verlag" where glass knives and tungsten
coating are discussed.
---The scoring wheel needs to be sharp.
---Does the geometry all work? Is the line that bisects the square in the
right place?
---Most of it lies in the glass. Is the long pre-fractured face of the
strip at right angles to the top/bottom face? Is it smooth? Is it clean?
Does the glass break readily with a small snap? Poor glass takes a lot of
force and breaks with a loud BONK. Bad because too much energy is
dissipated in the break.
---The clamping head that holds the scorer must slide up and down freely.
Light oil on the slide helps. Note the lever only clamps the movable slide
to the slideway. For the downward pressure to be the same each time
friction in the slide should be minimal so the mass of the scorer always
exerts the same force.
---To encourage uniformity, we use a "jiggle" with each clamping action.
Jiggle (the clamp!) when clamping the square to make the diagonal score.
Jiggle the scorer when lowering it onto the square to encourage good and
even contact.
---I don't know about the LKB 7800 knifemaker especifically, but I have
been make good knives in a Reichert knifemaker (for crioultramicrotomy).
Some basic aspects are very important in my opinion:
- Uniformity in the knife breakdown
---} Breakdown velocity (low = uniform = best edge).
---} Counter piece size (usually lower than 2 mm)
/|
/ |
/ |
/ |
counter piece-} |____|

---} knife angle - Usually 45o. if you need a "fine" edge, you can use a
smaller one (but less resistant edge) for sectionning of "hard" specimens,
the opposite.

*very important* ---} How to select the best knife?

Eye

/ |
/ |
/____| Knife


\|/

Light source


If you see the knife edge like a very fine (invisible? :)) line,
your knife is OK. You will see the edge like a bright line over a dark
field, in this system. 10 - 20% of EXCELLENT knives is a very good mark.


---Pay attention if the cutting (tungsten) disk is ok.
---Phase of the moon is important
---Not all microtomy glass is equal
---We are fortunate to have a gifted engineer in one of our workshops and
he has 'breathed' on our 7800 to remarkable effect. Our target was between
6 and 8 out of 10 good knives. Jan Slot, when he visited our laboratory,
said that he had never met anyone who had investigated the working of a
knifemaker as thoroughly. We published the mods; Journal of Microscopy,
Vol.168, Pt 1, October 1992, pp111-114. 'Further modification of the LKB
7800 series Knifemaker for improved reproducibility in breaking 'cryo'
knives'
---The LKB knife maker should be able to be adjusted to produce excellent
glass knives. Leica (Deerfield, IL) should be able to give you some kind
of assistance (what is left of LKB is owned by Leica).
---With regard to the knife glass, the LKB glass for many years the only
reliable source for really outstanding glass. Because of the very high
price, some other firms from time to time, came along offering glass of
inferior characteristics.
---Today, in addition to the glass that you could get from LKB, others are
offering glass that to the best that I can determine, would be
indistinguishable relative to the "standard" LKB glass. The glass that is
offered by Ted Pella and SPI are also found to be indistinguishable from
the LKB product. However, not all of the glass being represented as being
"good" for glass knives for thin sections comes from the same place. Some
people think there is only one source but that is just not true.
---The sharpness of the edges is the critical parameter in terms of
determining glass quality. That is why the glass comes so carefully
wrapped.
---Glass is very heavy and a very high percentage of the price you pay is
strictly related to transportation costs.
---The Messer Knife maker (Japan--imported by SPI) uses ordinary "float"
glass, either 5 mm or 6 mm thick, the kind that you can buy at your local
glass shop. It is very cheap to operate.
---We use an LKB 78018 knife maker, which I believe is the same idea as the
7800. After initial getting used to the thing I have had no problems at
all producing excellent knives (so much so that I often prefer them to
diamond knives, but for their durability of course). Just a few hints which
you probably know already: a) make sure the diamond cutter is not wobbly,
b) ensure that the holding block sits firmly on the glass and is secured
tightly, c) use only 'well matured' glass; the older the better (we are
using stock from the sixties supplied by LKB at the time), d) break in one
movement, without jerks, e) take care with the little rubber buffer, it
somtimes pushes the knives-to-be in too far, f) good luck (a very
scientific precondition to success...).
---No glass chips on the feet (obvious).
---No old lubricant binding up the clamping head as it drops. It should
slide down easily enough to clunk solidly on the strip if one is careless
with the handle.
---Clean, *sharp*, *freely-rotating* cutter wheel. If this is bad, simply
replacing it can make all the difference
---*NO* rotational play in the cutting slide. That's taken out by a
small, springy "finger" that rides in the slot on the underside of the
large stainless steel rod that is the cutting slide. Look at the back of
the head for the "finger" and its adjusting screw. We use an old one, and
found that replacing the cutting wheel gave it new life. We've also found
that simply asking our local glazier to let their best "free-break" person
cut up a 24" square of 1/4" plate into 1" strips works fine. We usually
have to throw away the 2" near the end of the break, but the guy takes
making these perfect strips as a personal challenge, and 24 1"x24" strips
currently costs us about $17.
---We had some luck in making glass knives with LKB glass. Also, the glass
was cleaned with 95% alcohol. When good knives were breaking - moon,
humidity, incantation - I stored the knives in a desiccator for up to two
years. Made a lot of them at one sitting.
---A key (you probably already know) is to check for any glass shards on
the pedestals, no vibrations, a slow break - even pressure, good score not
near edge of glass. Also I used 40 degree knives, not 45.
---I have had some of the same problems that you talked about. Right now
our knifemaker is doing a good job but that does not mean that the next
time it is used it will give good knives. When ours is misbehaving, I
usually spend a good part of the day making adjustments without any
improvements. Then suddenly and surprisingly, it gives good knives again.
This state may remain for a few days or months, if I am lucky.
---Coincidentally I had a visit from a salesperson recently and I mentioned
this to him and he told me about a SymParter that comes from Pelco. It is
an attachment that goes on the knifemaker to enhance its performance. You
might talk to the Ted Pella people about that.
---I think glass does make a difference and I have heard good things about
Pella glass. We purchase ours from Mager Scientific, Inc., P.O.Box 160,
Dexter, MI. 48130. I was planning to try Pella glass to alleviate our
problem but since we both have the same problems with different brands of
glass, the problem is probably not the glass.
---The old LKB glass is available from Ted Pella, and it really makes a
difference. Has your knifemaker had a good tuneup lately (scoring wheel,
clean slides of the head, caliper-measurement of the squares, fresh
dampening pad)?? Careful attention to all of the above can make a huge
difference in knife quality. I recently put in a nice fresh scoring wheel
and carefully tweaked the scoring pressure with a great increase in knife
yield (and consistency). Caliper-measure your squares!!









From: Marc Brande :      brande-at-SDSC.EDU
Date: Wed, 16 Aug 1995 10:47:59 -0700 (PDT)
Subject: Live Cell Imaging In Vivo

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I need to image fluorescently labelled live muscle cells in the living
mouse. Is this possible to do? If so, what are the possible imaging
technologies to accomplish this?

MRI microscopy?
Optical tomography?
Confocal?

Any input to get me started would be greatly appreciated. Thanks so much
in advance.

Marc


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From: gwe-at-biotech.ufl.edu (Greg Erdos)
Date: Wed, 16 Aug 1995 16:07:48 GMT
Subject: Re: Magnetic fields

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Earlier this year I solicited opinions about installing a 4.7 T NMR in the
vicinity of an EM lab. Below is the upshot of that installation and a
request from the NMR lab director for data on EM specs. Anyone who can be
helpful should respond

Thanks
}
} We've now completed our move and turned the magnet on, with no observable
} effect on EM performance (measured to 5.6 Angstrom resolution). On both
} the SEM and TEM, pre and post images were obtained, as well as magnetic
} field values measured pre, post, and during magnet energization. Both
} DC (with a Walker flux-gate magnetometer, Model FGM-3D1, 0.1-2000 mG
} range, DC-100 Hz response (200 mG and 2000 mG scales; DC-50 Hz on 20 mG
} scale), NIST calibrated and certified) and AC (Walker ELF-45D,
} 0.1-199.9 mG range, 30-300 Hz response) magnetic fields were measured.
}
} AC fields were 0.1-0.3 mG, and constant within that range, both before
} and after energization of our 4.7T (47 kG) passively shielded magnet.
} The earth's DC field in the hallway just outside the EM lab was about
} 400 mG (305 mG horizontal and 265 mG vertical) and varied by about 5 mG
} with time (several days). When the magnet was energized, a change of about
} 12 mG was noted (17 mG had been predicted). Changes in varous locations
} in the EM labs were 5-10 mG. The room was likely partially shielded by
} structural metal and equipment (including the EM instruments) in the rooms.
} The EM instruments themselves produce DC fields of from 100-1000 mG or more
} in the vicinity of the columns, where the focusing lens are located. The
} SEM had magnets on an ion getter pump within a foot of the top of the
} column which produced a DC field at the column of over 2000 mG.
}
} It is obvious the 5-12 mG stray DC field produced at the EM site by our
} NMR magnet is below the limits needed to cause problems for EM performance.
} Since the earth's DC magnetic field varies considerably with location
} (range at least 400-600 mG, and 150-300 mG horizontal, in the continental
} U.S.) and somewhat less with time, it is likely that EM instruments can
} compensate for stray, stable DC fields on the order of 100 mG or more.
} Anecdotal reports indicate that this capacity may be as much as 300-500 mG
} or more. The DC fields we measured near the EM columns corroborate these
} anecdotal reports.
}
} I would appreciate receiving, by fax or e-mail, any information on AC and
} (especially) DC field specifications that you might have for EM instruments.
} Please include the manufacturer, model number, type (e.g., SEM, TEM), and
} age of the instrument, as well as maximum magnification and accelerating
} voltage range. A faxed or mailed copy of the original manufacturer
} spec sheet would be most helpful.
}
}
}
} Richard W. Briggs, Ph.D.
} Dept. of Radiology, Univ. of Florida
} Box 100374, J.H. Miller Health Center
} Gainesville, FL 32610 U.S.A.
} tel: (904) 395-0680 ext 54279
} fax: (904) 395-0279
} e-mail: rbriggs-at-ufnmr1.health.ufl.edu
}
}
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
-at- Greg Erdos Phone: 904-392-1295
-at-
-at- Scientific Director,
-at-
-at- ICBR Electron Microscopy Core Lab
-at-
-at- 218 Carr Hall Fax 904-846-0251
-at-
-at- University of Florida E-mail: gwe-at-biotech.ufl.edu
-at-
-at- Gainesville, FL 32611
-at-
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-





From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 16 Aug 1995 17:06:00 -0400
Subject: RE-Vibes&MagFields

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Message-ID: {n1403529663.87073-at-mse.engin.umich.edu}

Subject: Time: 4:54 PM
OFFICE MEMO RE:Vibes&MagFields Date: 8/16/95

Those of you who are involved with problems involving mechanical vibrations
and magnetic fields might find it helpful to contact Wayne Vogen, President
of VEC Inc. (Ph: 510-339-8719). He specializes in measuring these
troublesome phenomena, and in finding methods for overcoming them, especially
in EM laboratories. I've seen him in action a couple of times, and have been
favourably impressed with his expertiese.





From: xin yang li :      xl48-at-uow.edu.au
Date: Thu, 17 Aug 1995 12:51:09 +1000 (EST)
Subject: Address of Epoxy Technology

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Hi, Dear Colleague.

I wonder if any one knows the address of Epoxy Technology. I want to buy
some Epo-Tek 353 ND epoxy for my XTEM samples but don't know the address of
the company.

Thanks for help

Xinyang

Dept. of Materials Engineering
University of Wollongong
Australia




From: GVKM07A-at-prodigy.com (DR CHARLES A GARBER)
Date: Thu, 17 Aug 1995 03:01:34 EDT
Subject: TEM Grid Stainers

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-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --

Martin Newman asked about "reliable TEM grid stainers".

The SPI Stain 'n Wash Grid Staining system is made primarily of glass
(as opposed to plastic) and is designed to use a very minimal volume
(e.g. 2-6 ml). It is usable for most heavy metal post-staining
techniques (thin sections) as well as immunochemical labeling or enzyme
localization techniques. Since one picture is still worth 10,000 words,
I would refer you to our electronic catalog on the WWW site given
below. Click "new products" and then "Stain 'n Wash".

I am told (by some of our customers) that the smaller than usual volume
needed results in meaningful cost savings when expensive reagents are
involved. One hundred grids can be processed at one time.

And any feed back I have ever received from customers has been quite
positive.

Chuck

======================================================
Charles A. Garber, Ph. D. Ph: 1-(800)-2424-SPI
President 1-(610)-436-5400
SPI SUPPLIES/Structure Probe, Inc. FAX: 1-(610) 436-5755
PO BOX 656
West Chester, PA 19381-0656 USA

e-mail: GVKM07A-at-prodigy.com [Direct for C. Garber]
SpiSupp-at-aol.com [SPI Customer Service e-mail box]

###########################################################
WWW Site: http://mail.cccbi.chester.pa.us/spi/spihome.html

###########################################################
======================================================





From: Ronald LHerault :      lherault-at-acs.bu.edu
Date: Thu, 17 Aug 1995 09:34:12 -0400 (EDT)
Subject: Millonigs-Thanks

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Thanks to those who gave me the info. I appreciate your help.

Ron





From: jmcgee-at-lunatic.er.usgs.gov (Jim McGee)
Date: Thu, 17 Aug 1995 09:56:34 -0400
Subject: Epo-tek

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The address of Epoxy Technology is:

Epoxy Technology, Inc.
14 Fortune Drive
Billerica, MA 01821 USA

phone: (508) 667-3805
fax: (508) 663-9782



Jim McGee
U.S. Geological Survey


------------------------------Reply Separator----------------------------------

I wonder if any one knows the address of Epoxy Technology. I want to buy
some Epo-Tek 353 ND epoxy for my XTEM samples but don't know the address of
the company.

Thanks for help

Xinyang

Dept. of Materials Engineering
University of Wollongong
Australia





From: Jeff Kingsley :      jkingsle-at-cea.com
Date: Wed, 16 Aug 1995 14:19:09 -0700
Subject: job posting

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The following message is a JOB POSTING for a SEM postion. Interested candidates
should send a resume or direct inquiries to Jeff Kingsley by e-mail at
jkingsley-at-cea.com, by FAX at 415 369-7921 or mail to 301 Chesapeake Drive,
Redwood City, CA 94063.
----- Forwarded message follows -----

Return-path: {jkingsle-at-cea.com}
Received: from cea.com by ENH.NIST.GOV (PMDF V5.0-4 #10952)
id {01HU5GMSDXWG00QLOK-at-ENH.NIST.GOV} for phelps-at-ENH.NIST.GOV; Wed,
16 Aug 1995 18:57:18 -0400 (EDT)
Received: from Connect2 Message Router by cea.com via Connect2-SMTP 4.00; Wed,
16 Aug 1995 15:58:46 -0700

Here is the job posting. Thank you for posting this on the listserv.



Charles Evans & Associates has an immediate opening for an experienced
FE-
SEM analyst in our Redwood City, California facility.

Charles Evans & Associates is a leading commercial analytical services
laboratory specializing in surface analysis and materials
characterization.
Charles Evans & Associates has a central laboratory located in Redwood
City,
California, and associated laboratories and representatives world-wide.
Currently
the laboratory has a staff of more than 75 personnel and over 20
analytical
instruments including SIMS, TOF-SIMS, AES, XPS, FE-SEM, SEM/EDS, RBS,
TXRF, GDMS, AFM and FTIR.

The current opening is involved primarily with the preparation, FE-SEM
imaging
and interpretation of cross sections of semiconductor devices. The ideal
candidate has 3-5 years of direct experience with semiconductor cross
sectioning
and FE-SEM imaging. The candidate must also have verbal and written
communications skills suitable for customer interaction and report
generation.
Interested candidates should send a resume or direct inquiries to

Charles Evans & Associates is an Affirmative Action/Equal Opportunity
Employer


----- End of Forwarded message -----





From: Jay Jerome :      jjerome-at-isnet.is.wfu.edu
Date: Thu, 17 Aug 1995 22:46:35 -0400 (EDT)
Subject: Re: Training Videos

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Message-Id: {199508171642.MAA00211-at-ns.ge.com}


The Education Committee of MSA has an extensive collection of VHS tapes
on microscopy. For the past 10 years the tutorial sessions at the MSA
annual meeting have been videotaped and are the backbone of the
collection. However, videos have been obtained from other sources as
well.
The tapes will soon be available for sale at a modest cost.
For a list of tapes or more information on acquiring them contact the
Chair of the Education Committee:

JoAn Hudson. She can be reached at
HJoan-at-clemson.edu

of check out the MSA Web Site

Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-isnet.is.wfu.edu *
**************************************************************

On Wed, 16 Aug 1995, Trevor Sewell wrote:

} Dear All,
}
} We would like to build up a library of training videos on aspects of
} microscopy. Does anyone know any good ones? I would also appreciate
} information on their current availability.
}
} Many Thanks
}
} Trevor Sewell
} EM Unit
} University of Cape Town
}
}




From: Rassie v Zyl: SEM :      RASSIE-at-INFRUIT.AGRIC.ZA
Date: Fri, 18 Aug 1995 08:13:27 GMT+2
Subject: unsubscribe

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Please unsubscribe - taking a break!



===========================================================
Rassie van Zyl
INFRUITEC EM Unit Tel: 021-8839090
PRIVATE BAG X5013 Fax: 021-8838669
STELLENBOSCH Internet: rassie-at-infruit.agric.za
7599
==============================================================




From: Jay Jerome :      jjerome-at-isnet.is.wfu.edu
Date: Fri, 18 Aug 1995 11:54:17 -0400 (EDT)
Subject: Some humor for the week-end

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The women broke from a tangled embrace to answe the phone. When she hung
up, her companion asked who it was.
"Mu husband," He was calling to say that he would be late because he was
bowling with you.






From: mullerw-at-rmslab.rockefeller.edu
Date: Fri, 18 Aug 1995 16:06:48 EDT
Subject: unsubscribe

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Please take me off the list (temporarily). Otherwise I will have 700 messages
clogging my e-mail box when I return from vacation. Thank you for this
courtesy. I'll resubscribe in the fall.

Bill Muller
The Rockefeller University





From: MicroToday-at-aol.com
Date: Sat, 19 Aug 1995 19:29:17 -0400
Subject: Used Equipment

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In the next issue of our newsletter "Microscopy Today" we offer you free
listings for either Used Equipment For Sale or Used Equipment Wanted.
Provide your CONCISE copy no later than 1 September by eMail or FAX
(608-836-1969.
And - should you not be the person responsible for purchasing used equipment
at your organization, kindly supply us the proper name and address and we
will see that he/she gets a no charge copy.
Regards to all -
Don Grimes, Microscopy Today




From: ldm2-at-apollo.numis.nwu.edu (L.D.Marks)
Date: Sun, 20 Aug 1995 12:08:03 -0500
Subject: Teaching using the Web ?

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I have received a number of encouraging responses to my previous
email about exploiting the web for teaching purposes. Exploring
the web a little myself this morning, it is clear that many people
are developing material which can be used, and I also talked to
a few people as MSA who are interested.

The Web is an ideal tool for a distributed development, where if
enough people contribute the effort becomes reasonable; developing
a full WWW EM course by yourself is manyears of work. Let me float the
idea of different people contributing small sections on different
techniques. (There is already some of this out there, but I am
going to email people directly to check that they are happy with
their links being commonly known.) Some possible areas are:
1) Basic electron optics
2) Sample preparation
3) Bright Field/Dark Field
4) Image Contrast from dislocations
5).......

These should be at the level for undergraduates, with additional
information available (or links) for graduate students.

There is also the question of small icons to keep interest and add
a little color. (Icons need someone with artistic flair.)

Please send me an email if you are interested in contributing something,
either in the future or a link that you already have. I will start
a central clearing house and we can talk further by email (off the
listserver).

Laurie Marks




From: Birkenmeie-at-aol.com
Date: Mon, 21 Aug 1995 00:30:12 -0400
Subject: Change of address

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Can anyone tell me how to change the address at which I receive mail from
this list server? Thanks.





From: William Tivol :      tivol-at-wadsworth.org
Date: Mon, 21 Aug 1995 10:19:35 -0400 (EDT)
Subject: Re: Teaching using the Web ?

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Dear Laurie,

} 5)....... I suggest Electron diffraction
}
} These should be at the level for undergraduates, with additional
} information available (or links) for graduate students.
}
I think I can handle this; however, I will not be able to include
CBED.

} There is also the question of small icons to keep interest and add
} a little color. (Icons need someone with artistic flair.)
}
Someone else will have to do my icon.

} Please send me an email if you are interested in contributing something,
} either in the future or a link that you already have.

Yours,
Bill Tivol




From: William Tivol :      tivol-at-wadsworth.org
Date: Mon, 21 Aug 1995 10:50:36 -0400 (EDT)
Subject: Re: EDS Detector HV Stability

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}
} I am interested in opinions concerning the relative merits
} of keeping an EDS HV bias on continiously, as opposed to
} shutting it off after every use. In other words, is there
} any advantage wrt to stability of the electronics or
} energy calibration to keeping it on?
}
Dear HHXS,
One problem could be that, since the detector is more succeptible to
radiation damage when the bias is on, unless your detector is very well
shielded, you will damage it if you leave the bias on. Of course, if your
instrument is dedicated to EDS--as opposed to a general-purpose 'scope
with an EDS used occasionally--there will not be any extra damage.
Yours,
Bill Tivol




From: Michael Cammer
Date: 8/14/95 9:42 AM
Subject: Re: Storage devices

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Message-Id: {n1403124347.49753-at-macmail7.lbl.gov}

Reply to: RE} } Storage devices

Reply to: Michael Cammer, Robert R. Wise

But how long have you been using them? We used 230MB internal drives but have found them incredibly sensitive to dust (presumably,
because they are internal drives, the Mac ventilation system continually drags air, and dust, through them). After two years,
everybody who used them has lost data.

Michael A. O'Keefe
Acting Head, NCEM
--------------------------------------

On Mon, 14 Aug 1995 wise-at-vaxa.cis.uwosh.edu wrote:

} To all,
}
} I have seen discussion of several of the various storage formats
} for archiving electronic images--i.e. read/write CD, magneto optical,
} tapes, etc. What are the collective thoughts on 3.5" cartridges (such as
} Syquest)? They can hold up to 270 Mb and would seem to be a cheap (~$65)
} alternative that can be read by any machine with a 3.5" drive.
}
} Bob
}
}
} Robert R. Wise, PhD
} Director, UWO Electron Microscope Facility
} Department of Biology
} University of Wisconsin Oshkosh
} Oshkosh, WI 54901
} (414) 424-3404
} (414) 424-1101 fax
} wise-at-vaxa.cis.uwosh.edu
}
}
}






From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-aaem.amc.anl.gov
Date: Mon, 21 Aug 1995 11:14:47 -0500 (CDT)
Subject: Video Tape Info

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To add to Jay Jerome's info on the MSA Video Tape Library
You can get a listing of all the tapes in the current
library on the WWW. Following the hot links from the
following site to the MSA Home Page.

http://www.amc.anl.gov

Go to the section on Microscopy Societies, then look for
the Video Tape library document.

Cheers.. Nestor
Your Friendly Neighborhood SysOp




From: tphillips-at-biosci.mbp.missouri.edu (Tom Phillips)
Date: Mon, 21 Aug 1995 17:35:28 -0600
Subject: Free Siemens 101 EM

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Message-Id: {v01510100ac5ec848ee61-at-[128.206.15.189]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Anyone want a fully functional ancient Siemens 101 TEM for free (you pay
costs of removal and shipping)? Lots of spare parts for anyone still
running one of these instruments. Contact me directly ASAP or it will be
removed as scrap.


Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility
3 Tucker Hall
University of Missouri
Columbia, MO 65211
(314)-882-4712 (voice)
(314)-882-0123 (fax)






From: Michael Cammer :      cammer-at-aecom.yu.edu
Date: Tue, 22 Aug 1995 10:16:38 -0400 (EDT)
Subject: Re: Storage devices

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The first one we purchased was when they first were selling the 230
instead of the 128, I think in the late spring of 1994. The first one we
received, a Fujitsu, was defective, but its replacement has worked fine
since then, over a year. We have lots of dust/soot in the Bronx, but
these external drives seem to work ok. Somebody had a disk with a loose
sticker on it so it got wedged int he drive; when I opened the drive (an
external) to get it out, dust build up did not seem nearly as bad as the
dust inside the computers themselves (I had to swap cards a few weeks
ago and the dust on the motherboards looked like that under Duchamp's
bed). We now also own two Olympus models. Other people around the
University have these drives too, and I have heard no complaints about
malfunction due to dust.
-Michael

On 21 Aug 1995, Michael OKeefe wrote:
} Reply to: Michael Cammer, Robert R. Wise
}
} But how long have you been using them? We used 230MB internal drives but have found them incredibly sensitive to dust (presumably,
} because they are internal drives, the Mac ventilation system continually drags air, and dust, through them). After two years,
} everybody who used them has lost data.




From: STEVEN E SLAP :      75767.640-at-compuserve.com
Date: 22 Aug 95 08:38:13 EDT
Subject: Re: Spurr's resin formula

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Microscopy Listserver {Microscopy-at-aaem.amc.anl.gov}

Dear Fred-
Yes, Spurrs can be polymerized in a microwave. There are a series of papers by
Beverly Giammara on the subject. I'll send you a bibliography if you like, and
also copy this reply to Beverly so she can respond to you directly. The
procedure is done in flat Silastic embedding molds (BEEM capsules will melt) in
about 30 minutes. We recommend use of a laboratory microwave with a vent
interlock system to avoid the possbility of your breathing the fumes from the
resin.
Best regards,
Steven Slap





From: STEVEN E SLAP :      75767.640-at-compuserve.com
Date: 22 Aug 95 09:35:59 EDT
Subject: Re: Training Videos

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Dear Trevor-
There is a whole library of videos available from the MSA Business Office.
Contact Larry Maser at mmaser-at-mbl.edu and he'll send you a catalog. We have
found them very useful.
Steven Slap





From: William Tivol :      tivol-at-wadsworth.org
Date: Tue, 22 Aug 1995 13:24:45 -0400 (EDT)
Subject: Peter Statham/Re: EDS detector HV & damage

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Dear Peter,
}
} I am curious about this reply. Why do you think radiation damage
} would be higher with the bias on?
}
I was told this by Kevex, and I accepted it without much thought. Now
that you bring it up, it would seem that the kinds of damage brought about by
a 1.2 MeV e- beam would occur independently of whether the bias was on. There
might be some affect on recombination, but this seems unlikely with the 500
volt setting for the bias. Possibly I just misunderstood Kevex. Maybe some-
one from Kevex will comment.
Yours,
Bill Tivol




From: James R. Stets :      stetsjr-at-ttown.apci.com
Date: Tue, 22 Aug 1995 14:17:26 -0400 (EDT)
Subject: TEM: Help with LKB Microtome Cryokit

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A colleague who does not have internet access is trying to set up the
cryo-attachment (LKB 14800 cryokit) for his LKB model 8800 Ultratome III.

If you can offer assistance in set-up and operation, he would appreciate it.

Please contact him directly:
Mr. Fred Mebus
Specialty Minerals, Inc.
640 N. 13th Street
Easton, PA 18042
phone 610 250-3363
Thank you.




From: delannoy-at-welchlink.welch.jhu.edu (Michael Delannoy)
Date: Tue, 22 Aug 1995 15:59:43 -0400
Subject: Melange chromic acid/cellulose membrane replica's

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To the helpful,
I'm trying to do platinum/carbon replication of cellulose membranes
containig 10 nm pores. We have to dissolve the membranes after
shadowing-where lies the problem. My partner heard of "melange
chromic acid" at a thesis defense which is supposedly bichromate
dissolved in sulfuric acid (what concentration?). She can't find bichromate
in the catalogs,so we need advice. Is there an alternative solution which
will dissolve cellulose membranes and how long does it take? Any suggesstions
or references will be greatly appreciated.

Michael Delannoy
Microscopy Facility
JHUMI





From: delannoy-at-welchlink.welch.jhu.edu (Michael Delannoy)
Date: Tue, 22 Aug 1995 16:09:51 -0400
Subject: Melange chromic acid/cellulose membrane replica's

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To the helpful,

I'm tying to do platinum-carbon replica's of cellulose membranes
containig 10 nm pore's and we have to dissolve away the membranes-where
lies the problem. My partner heard of "melange chromic acid" at a thesis
defense which is supposed to be bichromate dissolved in sulfuric acid
(what concentration?). She could not locate bichromate in any of the catalogs,
is there an alternative solution which will dissolve cellulose membranes
and how long does it take? Any suggestions or references will be greatly
appreciated.

Michael Delannoy
Microscopy Facility
JHUMI





From: David Dryden :      djd-at-electron.ph.unimelb.edu.au
Date: Wed, 23 Aug 1995 18:12:43 +1000
Subject: New WWW Site

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The Diffraction Physics Research Laboratory located at the School of Physics,
University of Melbourne, AUSTRALIA now has a Web page located at,

http://www.ph.unimelb.edu.au/~djd/diff-home.html

Feel free to give critism, advice etc with regard to these pages.

Regards,

David Dryden
School of Physics
University of Melbourne
Australia
djd-at-physics.unimelb.edu.au





From: William Tivol :      tivol-at-wadsworth.org
Date: Tue, 22 Aug 1995 17:03:22 -0500 (EDT)
Subject: Re: Melange chromic acid/cellulose membrane replica's

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} My partner heard of "melange
} chromic acid" at a thesis defense which is supposedly bichromate
} dissolved in sulfuric acid (what concentration?). She can't find bichromate
} in the catalogs,so we need advice.

Dear Michael,
Try potassium dichromate. The solution is the same as chromerge
(except possibly for concentration). The best concentration depends on
the use, and I'd guess that dissolving membranes does not require the
high-test. For dissolving silver from photo tanks I pour ~ 25 cm^3 of
potassium dichromate into ~1/2 tank (2 l) hot water. I add concentrated
H2SO4 (~50 ml) and top off with hot water. The residue dissolves almost
instantly. Be careful adding the acid to the water--NEVER ADD WATER TO
CONCENTRATED SULFURIC ACID. I'd suggest trying ~1% dichromate in 1 M
H2SO4 to start and changing the concentrations if necessary. BTW, a
stock solution of dichromate in 50% H2SO4 will be stable indefinitely,
so you may want to make this up first and mix with the appropriate
amounts of water and H2SO4 to get the various concentrations you need.
This mix is an oxidising acid! It will dissolve clothing (and, possibly
fingers) and will react explosively with organic materials--another
reason to start with low concentrations. Good luck & be careful.
Yours,
Bill Tivol




From: Henk.Kieft-at-ALGEM.PCM.WAU.NL (henk kieft)
Date: Wed, 23 Aug 1995 10:05:23 +0100 (CET)
Subject: re: Melange chromic acid/cellulose membrane replic a's

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Dear Michael.

I do not know if this is what you are looking for, but in our hands it works
fine. Please be careful when you prepare the mixture:

Take a flask of 250 ml
put in 100 ml of 10% sulfuric acid (H2SO4) and put it on ice.
carefully and slowly add 25 grams of chromic oxide (CrO3).
After dissolving all the chromic acid, we use this mixture to etch away our
material in a dilution 1part melange and 3 parts of water, and we let it
stand overnight before cleaning it with water carefully. But you can try
higher concentrations and play around with time. If you use higher
concentrations there might be a chance that you find crystals in your replica
afterwards. But give it a try. In our experiments on plant material we also
use in addition after careful washing 4% household bleach(unthickened). Just
give it a try.
If there are problems please let me know to see if I can be of some kind of
help


- - - - - - - - - - - - - - Original Message - - - - - - - - - - - - - -
To the helpful,

I'm tying to do platinum-carbon replica's of cellulose membranes
containig 10 nm pore's and we have to dissolve away the membranes-where
lies the problem. My partner heard of "melange chromic acid" at a thesis
defense which is supposed to be bichromate dissolved in sulfuric acid
(what concentration?). She could not locate bichromate in any of the
catalogs,
is there an alternative solution which will dissolve cellulose membranes
and how long does it take? Any suggestions or references will be greatly
appreciated.

Michael Delannoy
Microscopy Facility
JHUMI


- - - - - - - - - - - - End of Original Message - - - - - - - - - - - -



Henk Kieft
Dept Plantcytology and morphology
Wageningen Agricultural University
Arboretumlaan 4
6703 BD Wageningen
The Netherlands
tel: (+)31 8370 84863
fax: (+)31 8370 85005
E-mail: henk.kieft-at-algem.pcm.wau.nl




From: Mary Russell :      mary-at-debenres.demon.co.uk
Date: Wed, 23 Aug 1995 13:01:22 GMT
Subject: unsubscribe:mary@debenres.demon.co.uk

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unsubscribe


--
---------------------------------------------------------------------------
| Mary Russell EMail mary-at-debenres.demon.co.uk |
---------------------------------------------------------------------------




From: Goldmarker-at-aol.com
Date: Wed, 23 Aug 1995 06:59:10 -0400
Subject: What is Quetol?

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Message-Id: {9508231051.AA9988-at-pho018.sb.com}
To: Microscopy {Microscopy-at-aaem.amc.anl.gov}

Could someone tell me the chemical composition of Quetol or provide me with a
reference?

Thanks!

Donald P. Cox




From: delannoy-at-welchlink.welch.jhu.edu (Michael Delannoy)
Date: Wed, 23 Aug 1995 10:13:35 -0400
Subject: Here's my two oink's

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Hey Piggies,
I just got my E-mail up and running and have been reading these old
messages about the "game". All I know is that I had a great time playing
this year, and even had fun at the pig party (except for the "load dumping
incident"). Anyway I'll be back next year, as I hope everyone else will
(even Meeker), and would be proud to pit the pigs against any team as long
as we can really pitch with an impartial ump. Whatever the score, our team
never gave up (even against the Raiders), which I really didn't experience
with the 90-92 team (probably because we were to tanked to even walk!).
Anyway, until next season---} SUUUU WEEEE!

Mike D.





From: Tony McKenna - NZ Dairy Research Institute :      MCKENNA-at-ECCLES.NZDRI.Org.NZ
Date: 23 Aug 1995 13:13:38 +1200
Subject: Re: Chromic acid for cleaning Pt/C replica's

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You should be looking for dichromate in the catalogue.
I use chromic acid for cleaning replicas made from all sorts of food
preparations containing proteins and fat.

Dissolve 5g of sodium dichromate in 5ml water.
To this solution carefully add, with stirring, 100ml of concentrated sulphuric
acid.

The replicas will require about 24h for cleaning.

Hope this helps.

Tony McKenna
Food Science
New Zealand Dairy Research Institute




From: XEROSEN-at-CCVAX.FULLERTON.EDU
Date: Wed, 23 Aug 1995 14:13:55 -0800 (PST)
Subject: cleaning platinum/carbon replicas

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You can also use 1% sodium hypochlorite (bleach) to
clean tissue off of replicas. I use it when doing quick-freeze, deep-etchin gand rotary shadowing. The tissue I have used is eggs, btu the egg jelly
of the Xenopus is pretty tough and still dissolved in bleach for 1-3 hrs.

Eric A. Rosen
Department of Biological Sciences
Califrornia State University, Fullerton
Fullerton, CA 92624-9480




From: Ann-Fook Yang :      YANGA-at-EM.AGR.CA
Date: Wed, 23 Aug 1995 08:00:50 -0400
Subject: TN-5500 instruction video tape

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Message-Id: {s03ae404.014-at-EM.AGR.CA}
X-Mailer: Novell GroupWise 4.1

Does anyone know whether an instruction video tape is available
for Tracor Northern TN-5500 is available and where can I get it?

Ann Fook
Yanga-at-em.agr.ca





From: GVKM07A-at-prodigy.com (DR CHARLES A GARBER)
Date: Wed, 23 Aug 1995 17:50:16 EDT
Subject: Pt/C replication of cellulose membranes

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-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --

On August 22, Michael Delannoy wrote about problems with the
platinum/carbon replication of cellulose and imaging 10 nm pores.

Over the years, we have encountered this kind of a problem, that is,
with organic material "sticking" to the replica, preventing one from
being able to "see" through it by TEM. We encounter this kind of
problem not just for "ordinary" Pt/C replication situations, but also,
for removing organic type residues from freeze fracture "replicas". A
more common situation is the removal of polymer latex particles that
tend to "stick" in a freeze fracture situation.

We have found that the following approach offers advantages over any of
the "liquid" kinds of approaches, since the surface tension forces of
the liquid tend to disrupt the fine texture and pores one is trying to
resolve:

a) After replication with Pt/C [sometimes it is necessary to use only
Pt, in extreme cases], the "replica" is then "backed" with a layer of
SiO2, deposited by the evaporation of SiO from a tungsten basket. If
in a freeze fracture device, one would need another set of "posts" to
follow the Pt/C with the SiO coating.

b) One then does their best to remove the organics however else they
would try doing this, e.g. stripping, etc.

c) What is left is put into a plasma etcher (of the type made by SPI
Supplies would be our subjective preference, but ones made by others,
such as Fisons would probably work as well), and using oxygen only, and
typically for periods of time of less than one minute (ten seconds
might be all that is needed), all remaining organics are removed. The
reason for the "backing" with SiO2 (or who knows, maybe there is some
SiO as well) is that the oxygen plasma will not react with this second
layer, giving the replica coherence it would not otherwise have. The
Si is low enough in atomic number as not to result in any meaningful
loss of contrast.

d) One way or another, the replica is put onto a TEM grid and voila!,
and with a bit of luck, one sees what they are looking to see. Since
the grain size of the Pt when done as Pt/C is a bit under 1nm, the
resolution of the shadowing material should be good enough to at least
get a sense about 10 nm pores.

If it does not work the first time, don't worry, it does not work the
first time for anyone! Indeed if you think it did work the first time,
then you probably did something wrong. In any case, it is a "delicate"
procedure and you want to spend more than the normal amount of time
"validating" the procedure for your kinds of samples. Putting it
another way, you should, for this method, try to be your own worst
"devil's advocate" and make sure you have the answers to the questions
that will invariably come up.

Disclosure: SPI Supplies manufactures the Plasma Prep II plasma etcher
and is also a supplier of the silicon monoxide "chunks" used in this
procedure. Best results will be obtained by using tungsten baskets
instead of boats, which means you want silicon monoxide "chunks" and
not a powder.

This is the first time I have ever really "published" this bit of
secret "art", for removing the last vestiges of organics from a
replica. I would appreciate any feed back on your successes or
failures and/or ideas for improvements to the method.

Chuck

======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail:
GVKM07A-at-prodigy.com
West Chester, PA 19381-0656 USA Customer Service:
SpiSupp-at-aol.com

########################################################
WWW: http://mail.cccbi.chester.pa.us/spi/spihome.html
########################################################
======================================================





From: f.lawrence-at-qut.edu.au (Felicity Lawrence)
Date: Thu, 24 Aug 1995 09:10:33 +1000
Subject: EMSSA details

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Hi everybody.

I'm looking for details on the 34th Annual Conference of the EM Society of
Southern Africa. I have an email address (which I got off the net) but it
doesn't seem to be current (not recognisable at any rate). Although I also
have a postal address for Prof. M.E. Lee at the EM Unit in Sovenga SA., I
would prefer to contact him by e-mail or fax.

Many Thanks,

Felicity
EM Unit, QUT
Brisbane, Australia





From: xin yang li :      xl48-at-uow.edu.au
Date: Thu, 24 Aug 1995 09:41:55 +1000 (EST)
Subject: Thanks

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Hi, Dear colleagues.

Thanks to all the colleagues providing me the information of Epx-Tech.

Xinyang Li

Dept. of Materials Engineering
University of Wollongong
NSW, Australia




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-aaem.amc.anl.gov
Date: Wed, 23 Aug 1995 20:33:33 -0500 (CDT)
Subject: Meeting Info on EMSSA-95

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The only details that I have on the EMSSA-95 meeting are as follows:


34th Annual Conference of the Electron Microscopy Society of Southern Africa
(EMSSA 95)
Nov. 28 - Dec. 1 , 1995
Aventura Resort, Warmbaths, Northern Transvaal Province, South Africa
Contact: Prof ME Lee, EM Unit, University of the North, Private Bag X
1106, Sovenga 0727, South Africa.
E-mail: qemssa95-at-unin1.north.ac.za



----Nestor
Your Friendly Neighborhood SysOp




From: Bo Johansen :      BOJ-at-bot.ku.dk
Date: Thu, 24 Aug 1995 08:10:24 GMT+0200
Subject: Cleaning Pt/C replicas

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Michael Delannoy

If it is pure cellulose you are going to remove why not try a 0.1%
cellulase solution for 2-5 h. - In combination with pectinase it
works very well with plant material.

Bo

_____________________________________________________________________
Bo Johansen E-Mail: BoJ-at-bot.ku.dk
Botanical Laboratory Vioce: +45 3532 2167
Gothersgade 140 FAX: +45 3313 9104
DK-1123 Copenhagen K, Denmark http://boj.bot.ku.dk/www/staff/boj.htm
---------------------------------------------------------------------






From: Tao Shizong :      tao-at-solid.phys.ethz.ch
Date: Sat, 5 Aug 1995 11:32:54 GMT
Subject: unsubscribe

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unsubscribe microscopy




From: STEVEN E SLAP :      75767.640-at-compuserve.com
Date: 24 Aug 95 08:09:49 EDT
Subject: Re: re:EMSSA

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Microscopy Listserver {Microscopy-at-aaem.amc.anl.gov}

The information published gave the contact as Prof. Mike Lee, EM Unit, Univ. of
the North, Private Bag X1106, Sovenga 0727, South Africa; e-mail:
qemssa95-at-uninl.north.ac.za. This information was published in Microscopy Today.
Steven Slap





From: STEVEN E SLAP :      75767.640-at-compuserve.com
Date: 24 Aug 95 08:28:53 EDT
Subject: Re: Quetol

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Don-
There are at least three formulations of Quetol. The references are:
Quetol 653: Kushida, H, J Elec Mic, 29/2, 193, 1980
Quetol 651: Fujita et al, J Elec Mic, 23/2, 165, 1977
Quetol 812: Kushida, H, J Elec Mic, 32/1, 65, 1983
They are all made in Japan.
Steven Slap





From: Ann-Fook Yang :      YANGA-at-EM.AGR.CA
Date: Thu, 24 Aug 1995 09:17:55 -0400
Subject: Pt/C replication of cellulose membrane

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Message-Id: {s03c4a1a.042-at-EM.AGR.CA}
X-Mailer: Novell GroupWise 4.1

I read Dr. Charles A. Garber's reply to the above subject with
interset. I have not done plasma etching myself and would
appreciate some more details.

Could Dr. Garber provide details of how the Pt/C-SiO-organics was
supported during etching; whether the organic face was up and how
the clean replica was deposited onto a TEM grid. Many thanks.

Ann Fook
Yanga-at-em.agr.ca





From: kennedy-at-nsi.edu (grace kennedy)
Date: Thu, 24 Aug 1995 08:22:52 -0800
Subject: Quetol 651

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Quetol 651 is dibutyl glycidyl ether of ethylene glycol. For your
interest, DER 736 (additive to Spurr's) is dibutyl glycidyl ether of
polyethylene glycol. I'm curious to know why you ask--I have experienced
repeated problems with this resin in the last two years. Grace Kennedy






From: ACDUMAUA-at-INDYVAX.IUPUI.EDU
Date: Thu, 24 Aug 1995 10:29:01 -0500
Subject: unsubscribe

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unsubscribe acdumaua-at-indyvax.iupui.edu




From: mdphill-at-ppco.com (M. Dean Phillips)
Date: Thu, 24 Aug 1995 10:45:06 -0600
Subject: subscribe

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Subscribe Microscopy

***********************************************************
* M. Dean Phillips *
* Phillips Petroleum Company *
* e-mail: mdphill-at-ppco.com (internet) *
* snail mail: Phillips Research Center-Room 360PL *
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* Phone: 918-661-8733 *
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From: spignole-at-ix.netcom.com (Susanne Brandom)
Date: Thu, 24 Aug 1995 09:11:23 -0700
Subject: Email version of MicroWorld's News

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MicroWorld Resources and News is pleased to announce an e-mail version
of it's WWW newsletter. It will include a section on what is new on
the WWW, a list of the job openings and resumes that are on-line at
MicroWorld, microscopy news and press releases, and a list of upcoming
meetings. If you would like to submit an item, including information
about your WWW site, please send it to me. To receive the newsletter
by e-mail, return this message back to me. Inclusion of your real
name and affliation is optional. Please take care NOT to post the
return to this listserver.

All comments are welcomed.

Susanne Pignolet Brandom, Ph.D.
MicroWorld Resources and News
http://mwrn.ms.wwa.com/topfile.htm
spb-at-wwa.com or
spignole-at-ix.netcom.com





From: Fermin, Cesar :      fermin-at-tmc.tulane.edu
Date: 24 Aug 1995 07:02:41 U
Subject: Glycogen/histochem

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Message-ID: {n1402837011.15721-at-msmail.tmc.tulane.edu}

Could the colleague who posted a note about glycogen histochemistry a few
month ago send me at {fermin-at-tmc.tulane.edu} the receipe/references for
demonstrating the various configurations of glycogen in cells. A colleague
here at Tulane Medical school wants to look a human muscle glycogen. Thanks.




From: Steve Barlow :      sbarlow-at-sunstroke.sdsu.edu
Date: Thu, 24 Aug 1995 17:33:55 -0700
Subject: parts, MIKROS evaporator

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I'm looking for parts and schematics for a Mikros VE10 evaporator which
has come into my possession but needs work. Any suggestions of a source
of the aforementioned would be appreciated. You can reply to me directly
thanks
steve

----------------------------------------------------------
Dr. Steven Barlow
EM Facility/Biology Dept.
San Diego State University
San Diego CA 92182-0057
phone: (619) 594-4523
fax: (619) 594-5676
email to sbarlow-at-sunstroke.sdsu.edu





From: XEROSEN-at-CCVAX.FULLERTON.EDU
Date: Thu, 24 Aug 1995 21:17:31 -0800 (PST)
Subject: LM TRITC Lectins

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Can anyone help me with some lectin labeling problems I am having.
I am trying to label the tunicate egg ECM with a variety of TRITC conjugated
lectins purchased from EY Labs.
I am having a problem with the controls. The eggs are fixed in
3%Formaldehyde, 0/5% osmium and embedded in LR white resin. 15
one micron sections are cut and stuck to coverslips with which I immerse
in the solution containing the lectins and competative sugars.
The controls are prepared as follows:
1) the lectins are incubated in the competitive sugar that
is specific for it for 60 minutes at room temp, ranging in
concentration from .1mM to 1M and the sections are pre-incubated in the
sugar fro 30 minutes prior to adding the lectin/sugar mixture. These
are then incubated 60 minutes with agitation and then washed 45 minutes
in appropriate buffer.
The problem is the controls I think almost look like the experimentals.
The concentration of the lectin in the solution is 25 micrograms/ mL.

What am Idoing wrong if anything and does anyone have any suggestions?
I need to get this done by the August 31.

Cheers,

Eric A. Rosen
Department of Biology
California State University
Fullerton, CA 92634-9480
(714) 449- 5291
(714) 773-3426
xerosen-at-fullerton.edu




From: GVKM07A-at-prodigy.com (DR CHARLES A GARBER)
Date: Fri, 25 Aug 1995 01:53:57 EDT
Subject: Cleaning up of Pt/C replicas

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-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --

Ann-fook Yang (Aug. 24) asked for more clarification on the plasma
etching method:

There are two situations, each slightly different but sort of related:

a) Surface replications such as from the surface of a piece of paper
with exposed microfibers, so that when the Pt/C (or just Pt) is
shadowed onto the surface, a stripping agent is used (we prefer
polyacrylic acid, 1 or 2% aqueous), and when it is hardened into a
tough film and stripped off, enough of the fine fibrils are stripped
off as well, making it difficult (or impossible) to see through the
replica.

Our variation on the theme is to "back" the Pt/C, instead of with an
extra layer of carbon, we apply a layer of SiO2. From that point on,
one does the stripping, dissolves away the PAA leaving the "replica"
(but backed with SiO2) floating on the water surface. In this instance,
a piece can be picked up on a grid of your choice, and THEN the entire
grid is "etched" in a plasma etcher (not more than 100 watts power) for
maybe not more than 10 seconds (if using pure oxygen). The oxygen
plasma will etch away the fine fibrils clinging to the replica, but
will not "etch" the SiO2 support film (but if the support film was
carbon, it would eat away the carbon as well, and then there would be
no sample left). The exact etching times will vary depending on the
etching unit being used. We recommend a chamber geometry that features
a "manifold"design, to increase the etch rate and minimize the etch
time. If you can not guess which one (uniquely, so far as I know) has
that kind of design, e-mail me and I will tell you.

b) For freeze fracturing and etching, what I was thinking about was
the kind of sample such as suspended polymer latex (emulsion particles)
or even certain liquids with macromolecular viscosity modifiers (e.g.
certain heavy greases, motor oils with additives, etc), and again there
is this problem of having too much organic material clinging to the
replica. So if it is "backed" with SiO2 instead of C, it can be
"cleaned up" the same way, rendering otherwise non-useable replicas,
useable.

Common sense would dictate that the side to be etched should be "up"
but the reality is, from our experience, it does not seem to matter
because of the isotropic nature of the etching. Being primarily a
materials science person, I was responding from the perspective of
materials science applications, momentarily forgetting that there was a
life science perspective as well (e.g the clean up of biological
samples). Sorry about that.

It seems that the dry etching method has advantages when the material
to be removed will not respond to the chemical etchants or when fine
structures are involved for which surface tension effects could produce
artifacts. For the typical life science situations that apparently
most people are working with, I can not say that the dry etch method
would have advantages except possibly in the two types of instances
where noted.

I am trying to accumulate some kind of a list of publications where dry
plasma etching has been used to clean up replicas, so I would
appreciate by e-mail any such references. Thanks in advance.

Chuck


======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail:
GVKM07A-at-prodigy.com
West Chester, PA 19381-0656 USA Customer Service:
SpiSupp-at-aol.com

########################################################
WWW: http://mail.cccbi.chester.pa.us/spi/spihome.html
########################################################
======================================================






From: zpwang-at-befvax.uchicago.edu
Date: Fri, 25 Aug 1995 09:38:03 EDT
Subject: Subscribe

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From: NJWS-at-aol.com
Date: Fri, 25 Aug 1995 12:22:43 -0400
Subject: cryonova

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Looking to purchase cryonova cryoultramicrotomy system. If anyone knows of
availability please contact me
Thanks
Norm




From: chandler-at-lamar.ColoState.EDU (John Chandler)
Date: Fri, 25 Aug 1995 15:50:38 -0600
Subject: Re: Anti-Static Gun

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Message-Id: {199508252011.QAA29269-at-ns.ge.com}

} Does anyone know where to buy an anti-static gun for removing static charges
} during microtoming? Many thanks in advance.
}
}
} X. Zhang
}
} E-Mail: Xiao.Zhang-at-GEP.GE.COM
} Phone: (518)475-5241
} FAX: (518)475-5969

You might try a product called Zerostat. It has a gun type grip that, when
squeezed, distorts a piezo-electric crystal that discharges ions to
temporarily neutralize static. They used to be available at record shops
and from some of the EM suppliers. The most recent price I found in the
Ted Pella catalog was $55 each.

These also work great for getting rid of static when weighing powders and
pouring liquid embedding plastic components into plastic tri-pour beakers.

Hope this helps.

John
chandler-at-lamar.ColoState.EDU






From: Marija Gajdardziska Josisovska :      mgj-at-csd.uwm.edu
Date: Fri, 25 Aug 1995 16:50:56 -0500
Subject: TEM Tech Position @ UW-Milwaukee

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*********************POSITION VACANCY ANNOUNCEMENT***************************

Department of Physics, University of Wisconsin Milwaukee

Title: Electron Microscopist
(Instrumentation Specialist)
Appointment: Academic Staff 50%, Fixed Term
Salary Range: Salary is competitive
Application Deadline: September 8, 1995 (postmark)
Starting Date: September 25, 1995 (approximate)

Application Procedure: Send a letter of application, resume and
names addresses and telephone numbers of three references to:
Prof. Marija Gajdardziska
Department of Physics
University of Wisconsin Milwaukee
P. O. Box 413
Milwaukee WI 53201
Fax (414) 229 5589

Responsibilities: Daily operation, maintenance and user support of
the laboratory for high resolution electron microscopy and the
associated laboratory for specimen preparation. Duties include:

* Daily start-up of the instruments, alignment of the microscope,
basic maintenance and troubleshooting, scheduling and coordination
with manufacturers' service representatives;
* User training and assistance in: basic operation, alignement, high
resolution imaging, microanalysis, and specimen preparation; assistance
in laboratory section of graduate course in electron microoscopy;
* Preparation of specimen and operation of the microscope under
scientific guidance of faculty for approved academic and industrial
research projects;
* Assistance in scheduling and billing for microscope time, purchasing
of supplies for the microscopy laboratories, purchasing and installation
of instruments in future laboratory expansions and/or renovations,
establishment and implementation of records, regulations and procedures
for effective laboratory functioning.

Qualifications: B.S. or B.A. in general science, physics or
electronics with coursework and/or a minimum of 2 years experience in
electron microscopy. The successful candidate will have:

* Detailed knowledge of the operating principles of transmission electron
microscopes (TEM) with emphasis on physical sciences applications;
* Demonstrated ability to operate, maintain and troubleshoot a TEM;
* Demonstrated ability to train researchers how to use a TEM;
* Working knowledge of vacuum systems, TEM specimen holders and specimen
preparation;
* Elementary knowledge of crystallography, solid state matter and computers;
* Good communication skills for clear and efficient interaction with
faculty, students, technicians and service engineers;
* Experience in phase contrast imaging and diffractogram analysis is
desirable but ability to learn new techniques of imaging, diffraction and
spectroscopy is a must.

The Department of Physics and UWM are affirmative action, equal
opportunity employers. The names of those applicants who have not
requested that their identities be withheld and the names of all finalists
will be released upon request.
*******************************************************************************




From: MikGu-at-mme.liu.se (Mikael Gustafsson)
Date: Mon, 28 Aug 1995 09:46:44 +0200
Subject: Flat capillaries

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Alternate-Recipient: allowed
Auto-Forwarded: prohibited
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Sensitivity: Company-Confidential


I4m setting up an endothelial capillary culture system for microscopy
according to Cooke 1993. Does any one know where to find flat glass
capillaries with an optical quality suitable for high res microscopy ? Any
suggestions are welcome. Does any one have experience of such culture systems ?


=============================================
Mikael Gustafsson MD, PhD
Dept Med. Microbiology and
Dept Internal Medicine, Cardiology section
University Hospital of Linkoping
S 581 85 LINKOPING
SWEDEN

E-Mail: MikGu-at-mme.liu.se
FAX: 046/13/224789
Phone: 046/13/224783
=============================================





From: Me :      ylin-at-acpub.duke.edu
Date: Mon, 28 Aug 1995 08:06:13 -0400 (EDT)
Subject: Re: Storage devices

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microscopy-at-aaem.amc.anl.gov

Hello,

Have you considered the Omega drives? The Zip drive has 100 meg
cartridges and is rather inexpensive ($200 for drive and
~$20/cartridge). Also, they're about come out with their new Jazz drive,
which will have 1 Gig cartridges and, they claim, to have performance
close to HDs.

These are random access drives, BTW.

-Yiing Lin
ylin-at-acpub.duke.edu




From: Xiao Zhang
Date: Friday, August 25, 1995 4:11PM
Subject: Anti-Static Gun

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Does anyone know where to buy an anti-static gun for removing static charges
during microtoming? Many thanks in advance.


X. Zhang

E-Mail: Xiao.Zhang-at-GEP.GE.COM
Phone: (518)475-5241
FAX: (518)475-5969





From: _ _ _ _ _ MARK W. LUND _ _ _ _ _ :      LUNDM-at-PHYSC2.BYU.EDU
Date: Mon, 28 Aug 1995 10:46 MDT
Subject: Australian MAS conference

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Can anyone give me information about the Australian conference to
be held in Feb 1996?
regards
mark




From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 28 Aug 1995 17:44:34 -0400
Subject: RE-IUMAS in Australia

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Message-ID: {n1402490426.1340-at-mse.engin.umich.edu}

Subject: Time: 5:25 PM
OFFICE MEMO RE:IUMAS in Australia Date: 8/28/95

The meeting you inquired about is the First meeting of the International
Union of Microbeam Analysis Societies (IUMAS). It will be held at the
University of Sydney, Sydney, NSW Australi from Feb 5 to 9, 1996. It will be
preceeded by several workshops, and will be held in conjunction with the 14th
Conference of the Australian E.M. Society and the 9th. conference of the
Light Microscopy Society. Information can be found on WWW at:
http://www.bio.uts.edu.au./em.html;
or can be obtained from:
ACEM 14-microCosmopolitan, E.M. Unit, University of Sydney, N.S.W.
2006, Australia Ph: 61-2-351-2351 and Fx: 61-2-552-1967





From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 28 Aug 1995 17:52:27 -0400
Subject: RE-ChromicAcidCleanSoln

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Message-ID: {n1402490068.20832-at-mse.engin.umich.edu}

Subject: Time: 5:39 PM
OFFICE MEMO RE:ChromicAcidCleanSoln Date: 8/28/95

Chromic acid has been used for many years as a cleaning agent for glassware
in chemical laboratories. According to the 35th. Ed. of the Hancbook of
Chemistry & Physics (p. 2990), a solution appropriate for this purpose is
prepared by pouring (slowly, carefully, and with stirring) one litre of
concentrated sulfuric acid into 35 ml of a saturated aqueous solution of
sodium dichromate (Na2Cr2O7).





From: DRK-at-SHCC.ORG
Date: Mon, 28 Aug 1995 15:49:23 -0700 (PDT)
Subject: Unsubscribe Microscopy DRK@SHCC.Bitnet

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Please unsubscribe DRK.SHCC.BITNET

T




From: DRK-at-SHCC.ORG
Date: Mon, 28 Aug 1995 15:50:28 -0700 (PDT)
Subject: Subscribe Microscopy DRK@SHCC.ORG

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Subscribe Microsocpy DRK-at-SHCC.ORG

Many thanks, Doug Keene




From: m.dickson-at-unsw.edu.au (melvyn dickson)
Date: Tue, 29 Aug 1995 10:18:57
Subject: Re: Australian MAS conference

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To: microscopy-at-aaem.amc.anl.gov

In article "_ _ _ _ _ MARK W. LUND _ _ _ _ _" {LUNDM-at-PHYSC2.BYU.EDU} writes:
} Date: Mon, 28 Aug 1995 10:46 MDT
} From: "_ _ _ _ _ MARK W. LUND _ _ _ _ _" {LUNDM-at-PHYSC2.BYU.EDU}
} Subject: Australian MAS conference

} Can anyone give me information about the Australian conference to
} be held in Feb 1996?
} regards
} mark

The Conference World Wide Web page is:
http://www.bio.uts.edu.au/em.html

For registration etc, contact ACEM-14 ACTS, GPO Box 2200, CANBERRA, ACT
Australia 2601

Mel Dickson




From: oisydney-at-ozemail.com.au (Julie Sheffield-Parker)
Date: Tue, 29 Aug 1995 10:33:44 +1000
Subject: Re: Australian EM Conference

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} Can anyone give me information about the Australian conference to
} be held in Feb 1996?
} regards
} mark

The next Australian EM Conference ACEM-14 will be held at the University of
Sydney 5-9th February 1996. This will be a joint meeting with the 1st
International Conference of IUMAS (International Union of Microbeam Analysis
Societies). The conference is hosted by the Australian Society for Electron
Microscopy and the Microscopical Society of Australia.

The contact address for registration forms, fees and enquiries is:
ACEM-14
c/- ACTS, GPO Box 2200
Canberra, ACT 2601.
Tel (06) 257 3299 Fax: (06) 257 3256.
Sorry - Don't know if they have an e-mail address.


*************************************************
From:-

Julie Sheffield-Parker,
Oxford Instruments Pty. Ltd.,
P. O. Box 7,
Pennant Hills,
NSW 2120,
Sydney, AUSTRALIA

Tel: ++ 61 2 484 6108
Fax: ++ 61 2 484 1667
E-Mail: oisydney-at-ozemail.com.au

*************************************************





From: Kerry Gascoigne :      Kerry.Gascoigne-at-cc.flinders.edu.au
Date: Tue, 29 Aug 1995 16:24:44 +0930
Subject: SUBSCRIBE

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Message-Id: {9508290641.AA14935-at-gamgee.cc.flinders.edu.au}
Comments: Authenticated sender is {mnklg-at-[129.96.250.33]}

SUBSCRIBE
Kerry Gascoigne AROC Tech Cttee




From: W.L. Steffens :      STEFFENS.B-at-calc.vet.uga.edu
Date: Tue, 29 Aug 1995 08:26:06 EST
Subject: chromic acid

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This recent thread on preparing and using chromic acid solutions for
various cleaning purposes brings an important point to mind. The
qualities that make chromic acid such a good cleaning agent also make it
extremely hazardous to prepare, use, and store. Added to this, it is very
difficult to dispose of properly. Our environmental safety personelle
absolutely HATE it. For the past several years, they have been
encouraging laboratories to switch to more modern "equally effective"
means for cleaning. Has anyone else experienced this pressure to switch,
and has a suitable alternative been found?









-=W.L. Steffens=-
Department of Veterinary Pathology
College of Veterinary Medicine
University of Georgia




From: mgarment-at-facstaff.wisc.edu (Martin B. Garment)
Date: Tue, 29 Aug 1995 07:40:14 -0500
Subject: Antistatic gun

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Thanks to Jim Heuer for the info about the Static Master available from VWR.
The Zero Stat guns are no longer available from any of the EM suppliers as
far as I can tell, and I've been looking for a substitute source, or another
item.
Martin B. Garment
Ophthalmology and Visual Sciences
1300 University Ave. Rm. 6687
Madison WI 53706
Voice (608) 262-9596
Fax (608) 262-0479
Email - mgarment-at-facstaff.wisc.edu





From: W.L. Steffens :      STEFFENS.B-at-calc.vet.uga.edu
Date: Tue, 29 Aug 1995 09:15:15 EST
Subject: Staticmaster

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I have been using a device for the last 20 years or so to eliminate static
charges during microtomy. This device, the staticmaster is available from
many large photography supply houses. Hilton Mollenhauer introduced me to
it, and I have yet to find a better remedy to the static problem. This
device is a small cartridge, available in 3 sizes (I use the smallest).
It is meant to attach to a special camel-hair brush for removing dust
particles from negatives prior to printing. The cartridge itself consists
of an element containing a polonium source, an alpha emitter, and is
effective for about 5 years. I mount the cartridge (w/ double-stick tape)
as near as possible to the knife edge on the microtome...the result is no
static attraction problems.
Of course, since its a radiation source, it must be disposed of
properly when exhausted. It is available from: NRD Inc., Grand Island,
NY 14072 (800-525-8076). I have no interest in this company, I just
like the product.

-=W.L. Steffens=-
Department of Veterinary Pathology
College of Veterinary Medicine
University of Georgia




From: STEVEN E SLAP :      75767.640-at-compuserve.com
Date: 29 Aug 95 09:11:16 EDT
Subject: Re: Used JB-4 microtomes

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We are looking for used JB-4s, working or not, to cannibalize for spare parts.
Please contact me directly with any offers.
Steven Slap





From: vickie-at-macc.wisc.edu
Date: Tue, 29 Aug 1995 10:41:46 -0600
Subject: Symposium/Workshop Announcement

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Message-Id: {25082910420669-at-vms2.macc.wisc.edu}

Symposium on:

"Integrated Microscopy"

September 29 to October 1, 1995

Organized by:

Integrated Microscopy Resource (IMR)
a Biomedical Research Resource

at:

The Wisconsin Center
702 Langdon Street
Madison, WI 53706


PROGRAM

FRIDAY (EVENING), SEPTEMBER 29, 1995
6:00 - 7:55 OPENING RECEPTION
8:00 - 8:45 Ken Jacobson, Univ. N. Carolina-Chapel Hill
"Imaging the Traction Forces Exerted by Locomoting
Fish
Keratocytes"
8:50 - 9:35 John Sedat, Univ. California-San Francisco
"Multi-dimensional Optical Microscopy and EM Tomography:
New Approaches and Results"
9:40 - 10:25 Edward Salmon, Univ. N. Carolina-Chapel Hill
"Multi-mode Light Microscopy of Microtubule Assembly
Dynamics and Motor Proteins in Mitosis and
Related Movements"

SATURDAY, SEPTEMBER 30, 1995
8:30 - 9:15 D. Lansing Taylor, Carnegie-Mellon University
"Molecular Dynamics of Force Generation during
Cytokinesis"
9:20 - 10:05 Jeff Lichtman, Washington University
"Imaging Synaptic Competition in Living Animals"
10:10 - 10:40 COFFEE BREAK
10:45 - 11:30 John White, Univ. Wisconsin-Madison
"Evaluation of Multiple-Photon Excitation Fluorescence
Imaging for In Vivo Studies"
11:35 - 12:20 Steven Smith, Stanford University
"Dynamics of Hippocampal Dendrite Growth and
Synaptogenesis"
12:25 - 1:25 LUNCH (on your own)
1:30 - 2:15 Gary Borisy, Univ. Wisconsin-Madison
"Using Correlative Microscopy to Study the Dynamics
of
the Cytoskeleton"
2:20 - 3:05 Ralph Albrecht, Univ. Wisconsin-Madison
"Using Correlative Microscopy to Follow Cell Surface
Receptor Ligand Complexes and Associated
Subjacent Intracellular Events"
3:10 - 3:55 Paul Bridgman, Washington University-St. Louis
"Multiple Microscopic Techniques Applied to Growth Cone
Intrapodia Fixed During Live Observation"

5:00 - 6:00 EXHIBITOR'S SOCIAL
6:00 - 8:00 BUFFET DINNER

SUNDAY (MORINING), OCTOBER 1, 1995
9:00 - 9:45 Kent McDonald, Univ. California-Berkley
"Cryotechniques: New Hope for the Ultrastructurally
Challenged
9:50 - 10:35 Stanley Erlandsen, Univ. Minnesota
"Leukocyte Rolling and Transendothelial Migration:

Importance and Distribution of Cell Adhesion
Molecules"
10:40 - 11:10 COFFEE BREAK
11:15 - 12:00 Hans Ris, Univ. Wisconsin-Madison
"High-resolution FESEM Imaging of Internal Cell
Structures after Epon Extraction from Sections:
A New Approach to Correlative Ultrastructural
and Immunocytochemical Studies"
12:05 - 12:50 Eric Henderson, Iowa State University
"Biological AFM and Molecular Force Detection"

FEES:
General Registration $100.00 (On Site: $130.00)
(Includes: Opening Reception, Social and Buffet Dinner, Coffee
Breaks and Materials)

Local Registration $ 80.00 (On Site: $110.00)
(Includes: Opening Reception, Social, Coffee Breaks and

Materials)


FOR ADDITIONAL INFORMATION (including abstracts) CONSULT OUR WEB SITE

http://www.bocklabs.wisc.edu/imr/imr.html


TO RECEIVE A BROCHURE AND REGISTRATION FORM
SEND COMPLETE MAILING INFORMATION TO:

IMR
Univ. Wisconsin-Madison
1675 Observatory Drive
Madison, WI 53706

OR EMAIL: imradmin-at-calshp.cals.wisc.edu

#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*
Presentations will focus on biological problems for which a combination of
microscopies [i.e. integrated microscopy] has been used. The speakers will
demonstrate by example the power, potential and limitations of various
microscopical techniques. The techniques which will be discussed include:
DIC, Confocal, 2-Photon Excitation Imaging, SEM, TEM, Cryo-specimen
Preparation, and AFM.
#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*

********************************************************************************

Following the symposium, the IMR will be conducting a 2-day workshop (Oct 2
and 3). We will be presenting lectures and provide "hands-on" experience
for the following techniques:
* 2-photon excitation imaging
* 4D DIC imaging
* Cryo-SEM
* High pressure freezing
* Reversible embeddment for SEM and TEM

Workshop attendence will be limited to 25 participants. A letter of
application is required. Once accepted a fee of $150.00 will be due.

********************************************************************************





From: masur-at-msvax.mssm.edu
Date: Tue, 29 Aug 1995 11:52:40 -0500
Subject: Microinjection tools

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We need to purchase a microinjector system to label cultured fibroblasts
while viewing on a Leica CLSM. Can anyone recommend one which has worked
well for them and is not outrageously priced? Manufacturer, model and
price please

Thanks

Sandra K. Masur Box 1183
fax: 212-289-5945 Mount Sinai School of Medicine
phone: 212-241-6544 or 0089 NY NY 10029-6574
e-mail:masur-at-msvax.mssm.edu









From: :      MELLIOTT-at-prl.pulmonary.ubc.ca
Date: Tue, 29 Aug 1995 12:11:52 +0800PST
Subject:

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My apologies to the list, but, I have misplaced the address for
someone on the list server and need to get a hold of them. Could
Greg Martin at Johns Hopkins School of Med please email me. Thank you

Mark Elliott
UBC-Pulmonary Research Lab,
Vancouver BC
melliott-at-prl.pulmonary.ubc.ca




From: masur-at-msvax.mssm.edu
Date: Tue, 29 Aug 1995 15:52:57 -0500
Subject: NYSEM SYMPOSIUM:CELLS & ORGANELLES

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New York Society of Experimental Microscopists Presidential Symposium

in honor of the memory of Eric Holtzman
CELLS AND ORGANELLES Friday, September 22, 1995

9:00 REGISTRATION

9:30 WELCOME
Dr. David R. Colman, NYSEM president 1994-1995
Dr. Sandra K. Masur, NYSEM president 1995-1996

9:40 Dr. Ursula W. Goodenough, Washington University
The evolution of sex at a cellular level

10:30 Dr. Pamela Cowin, New York University School of Medicine
Cadherin-mediated cell adhesion

11:20 Dr. James E. Rothman, Memorial Sloan Kettering Cancer Center
Machinery and mechanisms of intracellular protein transport

12:10 LUNCH

2:00 Dr. Kathryn E. Howell, University of Colorado School of Medicine
New insights into the structure and function
of the trans-Golgi network

2:50 Dr. David D. Sabatini, New York University School of Medicine
The generation of transport vesicles in the trans-Golgi region

3:40 Dr. Paul B. Lazarow, Mount Sinai School of Medicine
Peroxisomes in yeast and man

4:30 Dr. Lorna Role, Columbia University College of Physicians & Surgeons
Development of synaptic transmission between neurons

5:20 RECEPTION

Co-sponsored by The Mount Sinai School of Medicine

For additional information:

Sandra K. Masur Box 1183
fax: 212-289-5945 Mount Sinai School of Medicine
phone: 212-241-6544 or 0089 NY NY 10029-6574
e-mail:masur-at-msvax.mssm.edu









From: Tom Taylor :      ttaylor-at-msai.mea.com
Date: Tue, 29 Aug 1995 17:11:55 -0400
Subject: Microscopy Listserver

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Please subscribe me to the listserver.
--
Regards,

Tom Taylor {ttaylor-at-msai.mea.com}




From: Michael Rock :      merock-at-u.washington.edu
Date: Tue, 29 Aug 1995 15:12:29 -0700 (PDT)
Subject: embedding problem

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I need advice with a project:
we are trying to evaluate bacteria which were grown on/in a porous
matrix made up of alumina/silicon
we want to examine the samples by TEM
is there a method or plastic which could infiltrate the cells, and also
be hard enough to allow thin (50nm) sectioning?

we have tried LR white (hard) but the matrix (very "brittle") splinters
away from the resin when thin sections are cut, if we section thicker
(100+ nm) sections .. they are too dense for the (100 kV) e-beam to penetrate

any help appreciated

Mike Rock
U.W. Zoology Dept.




From: XEROSEN-at-CCVAX.FULLERTON.EDU
Date: Tue, 29 Aug 1995 16:01:00 -0800 (PST)
Subject: unsubscribe

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unsubscribe me since the email address will be not operational anymore

xerosen-at-fullerton.edu




From: James Kelly :      jkelly-at-ENH.NIST.GOV
Date: Wed, 30 Aug 1995 09:15:41 -0400
Subject: Re: Need Information on Importing Data of Printout to a PC

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X. Wang wrote:

} Some time ago, I read an e-mail message that someone uses a SEM
} printout port (to a dot matrix printer) to transfer data to a PC.
} Currently, my Kevex Delta EDS system (PDP-11/TSX operating system) is
} connected to a PC through TCP/IP for transfering images and data files.
} But there is one type of data set in the Kevex system I can not save onto
} the disk and only stays in the memory. I can not transfer it to the PC.
} But I can print it out to a dot matrix printer (but not to data file)
} through the Digital Dec computer.
} I need your help on information how I can intercept the data to
} the dot matrix printer and acquire it into my PC. Could some tell me what
} kind of module or software I need in order to do this task?
}

I have successfully transferred data by connecting the printer output cable
to COM1 of my PC. It only required a 25 pin to 9 pin adapter. I run Kermit
on the PC. Give a Log Session command with a filename then connect(C). On
the Kevex side give the Print command. After the data have listed, hit Alt
X on the PC and close session.

Good Luck.
Jim Kelly
NIST





From: krogers-at-ecn.purdue.edu (kirk rogers)
Date: Wed, 30 Aug 1995 17:46:40 -0500
Subject: Re: embedding problem

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Message-Id: {v01530501ac6a99ae8b67-at-[128.46.155.230]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Recently Mike Rock posted:
} I need advice with a project:
} we are trying to evaluate bacteria which were grown on/in a porous
} matrix made up of alumina/silicon
} we want to examine the samples by TEM
} is there a method or plastic which could infiltrate the cells, and also
} be hard enough to allow thin (50nm) sectioning?
}
etc.

At Purdue we do some microtomy of hard materials (aluminum, steel, AlN,SiC)
and we have used Struers Epofix resin for specimen mounting. This resin is
expressly for mounting of relatively hard materials, although it sometimes
adheres best with some silane coupling agent that I can't think of at the
moment.

good luck,

Kirk

Kirk A. Rogers
krogers-at-materials.ecn.purdue.edu
317-494-8751 office http://materials.ecn.purdue.edu/~krogers/
(coming soon)
317-494-1204 fax
Purdue University, School of Materials Engineering,
1289 MSEE building, W. Lafayette, IN 47907-1289







From: William Tivol :      tivol-at-wadsworth.org
Date: Thu, 31 Aug 1995 14:21:27 -0400 (EDT)
Subject: Re: CryoEM: water-free liquid N2?

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Dear Michael,
You wrote:

} I was told of a device (a modified funnel with some special sieve in it?)
} used to remove contaminating moisture from liquid N2. It may be commercially
} available but I can't remember ever seeing anything like it. My colleague
} could not be more specific.

We have two LN2 set-ups which may be relevant. Our Gatan cryo-trans-
fer stage comes with a funnel with a sieve in it--Gatan may be able to give
you the name of a supplier or to sell you one separately. The only problem
with their funnel is (possibly) the size; its stem is ~1/4" o.d., so if you
need to deliver large amounts of LN2, this won't do it. Our main LN2 system
has liquid separators, so that only the liquid is sent down the pipe. These
consist of cups with small holes in the bottom, so that the liquid falls
through. We installed a home-made one on one fill line; we just took a plas-
tic cup and punched holes in it. If you don't find a commercial supplier,
and your shop can machine either perfluoro-polyethylene or polyethylene, have
them make a cup and put a stainless steel screen in it. The screen mesh
should be small enough to trap the ice, so it's pretty fine. Good luck.
Yours,
Bill Tivol




From: joan.hudson-at-ces.clemson.edu (JoAn Hudson)
Date: Thu, 31 Aug 1995 16:45:20 +0100
Subject: National Lab for testing of oxygen index and absorption tests.

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X-Sender: hjoan-at-ces.clemson.edu
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I am looking for a lab which is ASTM approved for testing oxygen index and
absorbtion. Additionally, I need a porosity # 50psi test as well. I am
looking for ASTM # D2863-77 and ASTM #D 570-81. If anyone has this
information please send me a name and phone number where I may have these
tests run. Any help will be very much appreciated. Thank you. JoAn
Hudson-at-ces.clemson.edu






From: joan.hudson-at-ces.clemson.edu (JoAn Hudson)
Date: Thu, 31 Aug 1995 16:50:45 +0100
Subject: National Lab for testing of oxygen index and absorption tests.

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I am looking for a lab which is ASTM approved for testing oxygen index and
absorbtion. Additionally, I need a porosity # 50psi test as well. I am
looking for ASTM # D2863-77 and ASTM #D 570-81. If anyone has this
information please send me a name and phone number where I may have these
tests run. Any help will be very much appreciated. Thank you. JoAn
Hudson-at-ces.clemson.edu






From: Richard E. Edelmann :      EDELMARE-at-CASMAIL.MUOHIO.EDU
Date: Thu, 31 Aug 1995 17:33:57 -500
Subject: CD-R's

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The September issue of Computer Shopper as a main feature article
on CD-R's (Recordable CD's) discussing 6 currently availible units
for under $2,000, as well as some very useful hints on actually
recording CD media and software.

If your interested you can either hunt down the article or check out
their web site WWW.ZIFF.COM which has a full search feature for
topics found in all the computer related Ziff-Davis journal
publications. (Access to the site is free, and registration to the
site is also free.)





From: Stefan.Gunnarsson-at-devbiol.uu.se (Stefan Gunnarsson)
Date: Fri, 1 Sep 1995 08:41:47 +0200
Subject: Re: CD READ/WRITE SYSTEMS

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} We are currently in the last throws of setting up a confocal microscope
} with all the related ancilliaries including the system required for mass
} storage of data. We were very keen to include a CD read/write system for
} collection and storage of the images. These appeared to be the way of the
} future for storage and for transfer from the confocal workstation to the
} many user sites on this campus. Much to our dismay we have found(heard)
} that these systems often `crash' the Silicon Graphics system we will be
} using to drive our imaging software. This has made us do an about face and
} move back towards an MO drive. Has anyone else faced this dilemma ? Can we
} still confidently go down the CD path.
} Can anyone out there give us some confidence boosting news?
} I would love to hear from you.
}
} Tony McKenna
} NZ Dairy Research

We have a Kodak CDR225 connected to a PowerMac 8100 and use a program
called Toast CD-ROM to write CDs. The files are mostly image files which we
fetch from our SG Indy by Fetch 2.1. We didn't consider putting the CDR on
the SG machine as we want to edit etc. the images in Photoshop on the
PowerMac. We usually write the CDs in the ISO 9660 format so that they can
be read by any computer. The setup works fine except that the Indy is
terribly slow in mounting the CDs afterwards, especially if there are many
(} 30-40) files in one folder. It may be something in the system of our
machine that's a bit screwed up, in which case I hope to get it fixed, but
still it is probably worth having in mind.

Stefan


..............................................................................

Stefan Gunnarsson
Microscopy Unit,Dept. of Animal Development and Genetics

Uppsala University
Norbyv. 18A, S-75236 UPPSALA, Sweden
e-mail Stefan.Gunnarsson-at-devbiol.uu.se








From: CHAFFEYN :      NIGEL.CHAFFEY-at-bbsrc.ac.uk
Date: Fri, 1 Sep 1995 13:49:21 +0000
Subject: Fluorescence microscopy -a sticky problem

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Via: uk.ac.bbsrc; Fri, 1 Sep 1995 13:50:33 +0100
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Dear Fellow Microscopists,
I am having great difficulty in getting methacrylate-embedded sections
to adhere to glass microscope slides. The slides are coated with 0.1%
poly-lysine (MW c. 19000). Sections, c. 6 or 8 um thick, in a drop of water
are dried down onto the slides for a few hours at c. 43C, then overnight at
room temperature. The resin is removed with 100% acetone and the sections
(still stuck at this stage) are hydrated in phosphate-buffered saline.
Sections are then processed for indirect immunofluorescence localisation of
tubulin or actin. However, a (very!!) high % os sections float off the slides
after the c. 45 min pre-incubation blocking stage. The plant material used is
secondary vascular tissue of roots of Aesculus hippocastanum L., ie a little
bit of bark, cambium, and a large 'slab' of xylem, in radial longitudinal
sections. In view of the heat-sensitivity of the tubulin antigen, I want to
avoid excessive heating of the slides. Can anybody suggest a fool-proof (or,
at least a tried and tested) way of ensuring that the sections stick and stay
stuck? Any help or comments would be most appreciated,

Nigel Chaffey, IACR - Long Ashton Research Station, Department of
Agricultural Sciences, University of Bristol, Long Ashton, Bristol BS18 9AF,
UK: nigel.chaffey-at-bbsrc.ac.uk




From: Ian Hall :      hall-at-me.udel.edu
Date: Fri, 1 Sep 1995 08:58:21 -0400 (EDT)
Subject: Re: CryoEM: water-free liquid N2?

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This will not be news to many of the old hands but one or two may
find it useful following the recent thread on water-free LN2. An effective
way of dealing with this for small quantities is to put a couple of 'Kimwipes'
in the funnel before you start pouring the LN2. They absorb/filter out
even very tiny ice crystals AND ARE CHEAP AND HANDY. They allow the LN2
through quite freely and, if the flow begins to slow down half way
through the fill, you can throw them out and put fresh ones in. No good
for automatic or large volume systems, of course.
Regards

Rick Hall
Materials Science
Univ. of Delaware




From: baskin-at-biosci.mbp.missouri.edu (Tobias Baskin)
Date: Fri, 1 Sep 1995 08:49:14 -0500
Subject: Re: Fluorescence microscopy -a sticky problem

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Nigel Chaffey wrote...

} Dear Fellow Microscopists,
} I am having great difficulty in getting methacrylate-embedded sections
} to adhere to glass microscope slides. The slides are coated with 0.1%
} poly-lysine (MW c. 19000). Sections, c. 6 or 8 um thick, in a drop of water
} are dried down onto the slides for a few hours at c. 43C, then overnight at
} room temperature. The resin is removed with 100% acetone and the sections
} (still stuck at this stage) are hydrated in phosphate-buffered saline.
} Sections are then processed for indirect immunofluorescence localisation of
} tubulin or actin. However, a (very!!) high % os sections float off the slides
} after the c. 45 min pre-incubation blocking stage. ....

We have been using this methacrylate system for localizing tubulin
and actin in plant roots for some time. We do not have problems adhering
our sections, before or after extraction. We prepare slides coated with 3
aminopropyltriethoxysilane. Then we collect sections in a drop (about 10
microlitres) of water and then treat the slides for 2-5 min on a hot plate
at 60 C, and then air dry at room temp usually overnight. This seems to
work great for us. The few minutes at 60 C seem to cause no trouble for our
staining. The silane is really easy to use. If you want to try it, I can
give you details of coating the slides. Hope this helps.

Tobias

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
___ ____ ^ ____ _____ Tobias I. Baskin
/ \ / / \ / \ / University of Missouri
/ | / / \ / / Biological Sciences
/___ / /__ /_____\ / /__ 109 Tucker Hall
/ / / \ ( / Columbia, MO 65211 USA
/ / / \ \ / voice: 314-882-0173
/ /____ / \ \____/ /_____ fax: 314-882-0123






From: David Henriks :      73531.1344-at-compuserve.com
Date: 01 Sep 95 13:16:45 EDT
Subject: 590 Tripod Polisher Workshop

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WORKSHOP OBJECTIVE: This course will cover all aspects of pre-thinning and
focus on final thinning via Tripod Polishing. Due to the limited class size and
the extensive hands-on opportunities, this course is well suited to novices as
well as advanced Tripodders. The course will include sections on:

How to do it and why should I?
What's really going on and what am I really seeing?
How to prepare small, specific area cross-sections.
The problem of widely differing materials (eg tungsten).
Rapid preparation of TEM cross-sections.
Preparation of a wide range of materials: semiconductors, ceramics, metals,...

HANDS ON OPPORTUNITY: This course will be unique in that it will provide a
hands-on opportunity for every class participant. Tripod Polishers, Polishing
Wheels, and pre-thinning equipment will be made available to all participants
and actual samples will be prepared - by the students - as part of the course.
This is a great opportunity to get your hands dirty and actually learn by doing.
The instructor will walk you through each step of the process and then let you
loose on the equipment. This course is designed to teach the Tripod Polishing
technique. Silicon samples will be provided to the students and used as the
basis for the course teaching.

WORKSHOP LOCATIONS AND DATES: South Bay Technology - San Clemente, CA
Dates:
November 10-11, 1995

INSTRUCTOR: Ron Anderson, IBM, East Fishkill Facility, Hopewell Junction, NY

CLASS SIZE: Due to the extensive hands-on aspects of this course, class size
will be strictly limited to 10 participants.

REGISTRATION FEE: $795.00 (includes lunches and Friday night dinner)
$ 695.00 if registration is paid by
October 1, 1995

REGISTRATION DEADLINE: October 15, 1995

For additional information please contact SOUTH BAY TECHNOLOGY at 800-SBT-2233,
Fax 714-492-1499 or e-mail: 73531.1344-at-CompuServe.com





From: baskin-at-biosci.mbp.missouri.edu (Tobias Baskin)
Date: Fri, 1 Sep 1995 15:05:58 -0500
Subject: LM/Silane treatment/stickiness

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Dear net,
Following the tread about making slides sticky, I received several
requests for the protocol for silanizing slides. So I am replying at large
to the net in case others are also interested. I got this protocol from
another lab on campus (Tom Phillips) and I have never modified it. So, if
it doesn't work for you, I probably can't help. Anyway, here it is:

The silane compound comes as a liquid from sigma. Its name is:3,
aminopropyltriethoxy silane

Dip slides in a 2% silane/acetone solution for one minute
Dip slides in 100% actetone for 1 min.
Dip slides in ddH2O for 1 min.
Repeat this step with agitation
Air dry.

The slides seem consistently sticky in our hands, although we once had
trouble with really teeny weeny sections that someone here was cutting (but
this was also for an aplication with lots of long rinses in running water).


Hope this helps. A citation and a little bit of further information
about this can be found in:

Angerer, L.M. & Angerer, R.C. (1991) Localization of mRNAs by in
situ hybridization. Functional Organization of the Nucleus: a Laboratory
Guide. Methods in Cell Biology, Vol 35, (ed. by B.A. Hamalko & S.C.R.
Elgin), pp. 37 - 71. Academic Press, San Diego.

Hope this helps, and best of luck.

Tobias

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
___ ____ ^ ____ _____ Tobias I. Baskin
/ \ / / \ / \ / University of Missouri
/ | / / \ / / Biological Sciences
/___ / /__ /_____\ / /__ 109 Tucker Hall
/ / / \ ( / Columbia, MO 65211 USA
/ / / \ \ / voice: 314-882-0173
/ /____ / \ \____/ /_____ fax: 314-882-0123






From: HEROUX :      CXHX%MUSICA.MCGILL.CA-at-ANLVM.CTD.ANL.GOV
Date: Fri, 01 Sep 1995 18:27:06 EDT
Subject: Used parts for Phase Contrast on Leitz Diavert

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Message-Id: {01SEP95.19928021.0292.MUSIC-at-MUSICA.MCGILL.CA}

Dear List Subscriber,
I would be interested in obtaining phase contrast
accessories for a Leitz Diavert optical microscope.
Specifically, the Phase Contrast Condenser according
to Zernike (513-84) would be ideal for our work.
These parts are on longer manufactured by Leitz.
We are also interested in Phase Contrast rings for
the Diavert.
The Diavert used to be the top of the line Leitz
microscope, 10 to 20 yrs ago. If you have some of
these units still around, but inactive, we could
make use of some of the optical parts --- and even
unload off you (at a reasonable price) major optical
components to repair our own unit.
Paul Heroux
Medicine
McGill University




From: bjg-at-uniwa.uwa.edu.au (Brendon J. Griffin)
Date: Sat, 2 Sep 1995 15:47:56 +0800
Subject: Used parts for Phase Contrast on Leitz Diavert

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Message-Id: {199509020736.PAA17042-at-uniwa.uwa.edu.au}

unsubscribe bjg-at-uniwa.uwa.edu.au
Brendon J. Griffin
Centre for Microscopy and Microanalysis
The University of Western Australia
Nedlands, WA, AUSTRALIA 6907
ph 61-9-380-2739 fax 61-9-380-1087





From: Carlos E. Barbosa :      grial-at-relay.starnet.net.ar
Date: 09/03/95
Subject: LM - petrology of sedimentary rocks

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From: Doug Davis :      doug_davis-at-maillink.berkeley.edu
Date: 3 Sep 1995 11:59:31 -0700
Subject: SEM mounting

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Message-ID: {n1401993263.29854-at-maillink.berkeley.edu}

Subject: Time: 11:54 AM
OFFICE MEMO SEM mounting Date: 9/3/95

Microscopy subscribers-
I understand Duco brand cement is sometimes used for mounting specimens for
SEM imaging.
Anyone have experience using this? I would like to know what its general
properties are- Cure time, outgassing, volatility under the beam, etc. Thanks
all.
doug_davis-at-maillink.berkeley.edu
EML, UC Berkeley





From: ANDRADY-at-RTI.ORG
Date: Tue, 05 Sep 1995 10:00:42 -0400 (EDT)
Subject:

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SUBSCRIBE Please.




From: ANDRADY-at-RTI.ORG
Date: Tue, 05 Sep 1995 10:01:14 -0400 (EDT)
Subject:

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Please UNSUBSCRIBE.




From: Giles John E Jr :      giles_john_e_jr-at-space.honeywell.com
Date: 5 Sep 1995 11:41:02 U
Subject: Solder Analysis

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Hello,

I am interested on the community input on performing in-situ analysis of solder
to determine composition. We have tried to do this in the past and have not
had acceptable results. We used both multiple areas and multiple points and
averaged the data.

I realize that this is probably an impossible task with EDS, but would like to
confirm my opinion and obtain input as to any other techniques (micro probe
XRF, etc.) which may provide a non-destructive method for in-situ solder
analysis.

Thanks,

John Giles
jegiles-at-space.honeywell.com




From: Mike Witcomb :      MIKEW-at-gecko.biol.wits.ac.za
Date: Tue, 5 Sep 1995 18:26:54 GMT+2
Subject: Electropolishing high speed steel

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A student wants to electropolish a 1cm square of M2 high speed steel -
he says this contains Mo, W and C. I would be grateful for a
solution composition, temperature and voltage suggestion.
Thanks in advance.
Mike


Dr MJ Witcomb
Electron Microscope Unit
University of the Witwatersrand
Private Bag 3
WITS
2050
South Africa

Telephone: + 27 11 716 4000
+ 27 11 716 2419 (messages)
Fax: + 27 11 339 3407
E-mail: mikew-at-gecko.biol.wits.ac.za




From: dlb-at-u.Arizona.EDU (David Bentley)
Date: Tue, 5 Sep 1995 10:24:09 -0700
Subject: Re: SEM mounting

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I haven't used the Duco cement in a long time. Since it is a
solvent based cement, it will have a long drying time and the danger of
rewetting dry biological samples. If memory serves, there was no great
problem with outgassing after the glue was dried thoughly. It was used for
a larger sample so the beam was not on the adhesive often, but I do remember
trials where some damage was evident.

There was a fantastic, comprehensive, review article by Judy Murphy that
covers most mounting techniques well;

Judith A. Murphy. Considerations, Materials, and Procedures
for Specimen Mounting Prior to Scanning Electron Microscopic Examination.
Scanning Electron Microscopy 1982 II. SEM Inc. Chicago. 1982. 657-696.

Generally, with biological samples and impervious samples, I shy
away from solvent based adhesives. The former rewet and suffer surface
tension artifact on redrying, and the latter trap liquid adhesive underneath
and outgas for a long time. Usually, like to stay with the coated adhesives
like the transfer tabs (Avery "spot o' glue"), metal tapes, and for tiny
samples Scotch 850 polyester tape, so the samples don't sink, or form ripples.

Sorry for the rant.
later dlb

David Bentley
Imaging Facility, Div. of Biotechnology, ARL
The University of Arizona
Tucson, Az 85721





From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Tue, 5 Sep 1995 14:04:14 -0700
Subject: EDS Detector Conversion

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Greetings:

We recently changed microscopes but retained our old EDS detectors. I am
working on finding the best way to put some of our old EDS things on the
'new' microscope. The conversion may require changing windows and/or
converting from a horizontal to a high angle configuration on a TEM.

Can anyone offer some sage advice about strategies, configurations, prices,
and/or vendors who can perform the work?

Thanks,

Jonathan Krupp
Microscopy and Imaging Lab
University of California
Santa Cruz, CA 95064
(408) 459-2477
jmkrupp-at-cats.ucsc.edu






From: STEVEN E SLAP :      75767.640-at-compuserve.com
Date: 06 Sep 95 07:38:27 EDT
Subject: Re: SEM mounting

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David-
The Avery "spot o glue" is no longer made, and hasn't been available in some
time. Check with your favorite consumables supplier for their recommended
substitute.
Steven Slap, Energy Beam Sciences





From: GVKM07A-at-prodigy.com (DR CHARLES A GARBER)
Date: Wed, 06 Sep 1995 10:55:31 EDT
Subject: SEM mounting

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X-Nupop-Charset: English

-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --

On Sept. 3, Doug Davis asked about "Duco" brand cement:

"Duco" cement will certainly "work", however, there are much better
alternatives, "better" being defined from several perspectives:

a) Double sided adhesive conductive carbon sheets and tape will do the
same job (provided the samples being mounted are not too large) but
have the advantage of no outgassing. They are also "relatively" stable
under the beam, and since the substrate is already conductive, to
whatever degree one might have to apply gold or carbon to impart
surface conductivity, in this case, one would have to apply much less.
Cure time is not applicable, and the sample is instantly ready to be
put into the vacuum of either a sputter or carbon coater or the SEM.
Remember, Duco cement is certainly not conductive. Also, if not
completely cured (who has the time for a complete "cure" in a busy SEM
lab?), solvent vapors off-gas.

b) One can use products called "Leit-C-Plast" or even "Tempfix" which
have the advantage that more massive samples can be held in place and
the adhesive itself is conductive. Again, cure time is not an issue.


The only apparent "disadvantage" is that these other alternatives all
cost more money than "Duco" cement. On a "per sample" basis, we are
still talking pennies, but they do cost more.

Now for this disclosure: SPI Supplies has been offering these "Duco"
cement alternatives for some number of years. They are in wide use
worldwide. Other types of dry adhesive products are available from
other suppliers, not necessarily the same item in a different wrapper.
They differ in terms of conductivity, purity, surface smoothness, and
freedom from pin-holes and of course, price. You have to try them out
to see which ones work best for you. All would seem to be a better
choice than "Duco" cement (or even any of the so-called "five minute
epoxies"). Products like "Duco" might seem to be "cheaper" but in the
long run, in many cases, they tend to be more "expensive" in terms of
wasted time doing samples over again and also, in terms of more
frequent column cleanings, aperture replacements,etc.

See pages 43-47 of the SPI Supplies "SourceBook" and/or contact SPI by
e-mail for appropriate SPI #'s and current prices. Sorry, these
products are not yet up in the SPI Supplies "electronic catalog" on our
"web site" but check after Oct. 1.

Chuck

=====================================================
Charles A. Garber, Ph. D. Ph: 1-(800)-2424-SPI
President 1-(610)-436-5400
SPI SUPPLIES/Structure Probe, Inc. FAX: 1-(610) 436-5755
PO BOX 656
West Chester, PA 19381-0656 USA

e-mail: GVKM07A-at-prodigy.com [Direct for C. Garber]
SpiSupp-at-aol.com [SPI Customer Service e-mail box]

###########################################################
WWW Site: http://mail.cccbi.chester.pa.us/spi/spihome.html

###########################################################
======================================================








From: jacobb-at-mh1.lbl.gov (Jacob Bastacky)
Date: Wed, 6 Sep 1995 10:09:34 -0800
Subject: mounting specimens for low-temperature SEM

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On Sept. 6 Alwyn Eades inquired about adhesives for mounting samples for
cryo SEM:

We have found that mechanical mounting in the equivalent of a tiny
vise built into the appropriate specimen stub makes for rapid mounting,
good thermal contact, and good stability under the beam at low temperatures
(down to that of liquid nitrogen in our case. Adhesives, including
"cryoglues" such as toluene failed or were awkward. Good thermal contact
has been critical in our experience so we fracture or saw cut at least one
or two flat faces on the sample to make contact with the jaws of the vise.

A photograph and diagram of a 12 mm holder we use can be found in
"Bastacky, J., C. Goodman, and T. L. Hayes (1990). A specimen holder for
low-temperature scanning electron microscopy. Journal of Electron
Microscopy Technique 14(1): 83-84." A 5mm holder for the Gatan coldstage is
described in "J. Bastacky, C. Lee, T. Freeman, G. Weber, A. Baeza, T.
Hubbins, Y. Chen. (1995). A specimen holder for high-resolution
low-temperature scanning electron microscopy. Microscopy Research and
Technique, in press." The technique of sawing flat faces is illustrated in
Bastacky, J., G. R. Hook, G. L. Finch, J. Goerke, and T. L. Hayes (1987).
Low-temperature scanning electron microscopy of frozen hydrated mouse lung.
Scanning 9: 57-70. I expect these approaches would work for liquid helium
temperature samples.

Jacob

Jacob Bastacky, MD
Room 116 Donner Laboratory
Lawrence Berkeley Laboratory EMail: sjbastacky-at-lbl.gov
University of California Phone: (510) 486-4606
Berkeley, California 94720 Fax: (510) 486-4750






From: delannoy-at-welchlink.welch.jhu.edu (Michael Delannoy)
Date: Wed, 6 Sep 1995 14:20:46 -0400
Subject: Non-microscopy related apology

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To the annoyed,
Whoops- sorry about the "My two oinks" mistake, please be assured
it will NEVER happen again. Thanks to all for the replica advice.

Mike D.





From: Larry Allard :      allardlfjr-at-ma160.ms.ornl.gov
Date: 6 Sep 1995 15:39:04 U
Subject: EDS detector HV stability

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Subject: Time: 3:35 PM
OFFICE MEMO EDS detector HV stability Date: 9/6/95

We never turn our EDS detectors off, and depend on the automatic shutdown in
the rare occasions that the LN2 is depleted. The only question I have about
turning them on and off routinely is whether the stability of the calibration
is maintained after numerous shutdowns and re-starts. Anyone have any
observations?
Larry





From: JOHNA-at-SCI.WFEB.EDU
Date: Wed, 06 Sep 1995 15:46:19 -0400 (EDT)
Subject: Job related injury?

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Hello folks,

I have just undergone heavy duty surgery on my right shoulder: tremendous
arthritis, bone spurrs, etc. etc. We did the right shouder because I am
right handed and because it was much worse than the left which will
probably need to be done in a year or two. Someone just said to me that
all electron microscopists end up with shoulder problems. I had not heard
this before. I've been doing EM full time for well over twenty years so I
guess I qualify as a "lifer". Have any of you EMers out there ever heard
of anything like this or have you experienced similar proble pro I'm just
curious; if it's true we should warn new EMers and perhaps get the
manufacturers to institute design changes to ameliorate such oblems.

Any thoughts?

John A.





From: tphillips-at-biosci.mbp.missouri.edu (Tom Phillips)
Date: Wed, 6 Sep 1995 15:25:05 -0600
Subject: filamentous phage TEM neg stain

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As a favor for a collaborator, I used PTA to negatively stain a
phage prep. I found lots of example of what I think may be filamentous
phages but am not certain. are there any phage morphologists out in cyber
space? I am interested in knowing the size range I should be seeing. my
filaments are about 12 nm wide. is this too big? can anyone suggest
references with good TEM pictures with which to compare my stuff? Thanks
for any help.


Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility
3 Tucker Hall
University of Missouri
Columbia, MO 65211
(314)-882-4712 (voice)
(314)-882-0123 (fax)






From: SWAINB :      SWAINB#a#PATHOL.DOS#u#MAIL-at-smtpgw.musc.edu
Date: 6 Sep 1995 16:31:45 -0500
Subject: Job Related Injury?

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Message-ID: {n1401717041.2028-at-smtpgw.musc.edu}

Mail*Link(r) MHS Job Related Injury?

I have questioned colleagues at this location and nobody has heard of an
EM related shoulder injury (we are all 20+years). A service engineer
visiting
us today does relate having heard of wrist related pain following repeated
exposure to freon during cleaning procedures..






From: akracher-at-iastate.edu
Date: Wed, 06 Sep 1995 16:33:53 CDT
Subject: Re: SEM mounting

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Message-Id: {9509062133.AA29067-at-las2.iastate.edu}
To: GVKM07A-at-prodigy.com
Cc: MICROSCOPY-at-AAEM.AMC.ANL.GOV
{013.00881436.GVKM07A-at-prodigy.com}

Let me second Charles Garber's comments on conductive/adhesive mounting.
I have had to run samples of powders applied to double-sticky "carbon
dots" on several occasions when it was impossible to coat the samples at
all. I don't recommend it, but you can get it to work. If anyone has a
similarly tricky SEM or EPMA problem, I'd be happy to supply more
information.
---
Alfred Kracher
akracher-at-iastate.edu
http://www.public.iastate.edu/~akracher/




From: SGKCCK-at-aol.com
Date: Wed, 6 Sep 1995 18:29:02 -0400
Subject: avery spot o glue-SEM mounting

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This is in response to Dr. Bently's message regarding the Avery Spot-O-Glue.
It is absolutely still in existence and never was unavailable as was posted
on the listserver. Two years ago Avery discontinued making the product and
we at Electron Microscopy Sciences bought out the equipment needed to
manufacture the Spot-o-Glue and have been manufacturing it under the same
name for the past 2 years. It still comes in the 2,592 tabs per pack with 72
sheets per pack. The pricing remains as it was when Avery was making it.
If anyone wants further information on the product please just give me a call
and I will be more than happy to assist.
Sincerely,

Stacie Kirsch
SGKCCK-at-aol.com
ELECTRON MICROSCOPY SCIENCES
TEL:215-646-1566
FAX:215-646-8931




From: R.Garlick-at-qut.edu.au (Rachel Garlick)
Date: Thu, 07 Sep 1995 12:48:58 +1000
Subject: Need Help with Cryosectioning

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Dear Colleagues,

I apologize in advance for this lengthy letter but I'm in desperate need of
help & feel it is necessary to describe the whole picture.

I am performing cryoultramicrotomy of wool fibres which have been treated
previously with a stain to provide sufficient contrast to observe
ultrastructure & the changes which take place as the water in the wool fibre
is dried. The aim is to compare ultrastructure of frozen hydrated & freeze
dried cryosections of wool to determine where water is located/contained in
the fibre.

A method has been established during a number of trials based on different
cryogens, embedment methods & media, temperatures, knife angles, collection
methods etc. To summarize, an RMC MT7 ultramicrotome fitted with CR-21
cryoattachment, is used at a temperature (both specimen & knife) of -125=B0C=
,
45=B0 glass knives with cutting angle of 5=B0 and dry collection (because=
frozen
hydrated sections required) of cryosections with eyelash probe onto Cu
sandwich grids coated with celloidin & carbon.

The embedment method uses a plastic (Gilson's 2-100=B5l) pipette tip to=
clamp
a bundle of fibres which protrude from the tip. It is necessary to use a
bundle of fibres to provide sufficient support - wool fibres do not freeze
very well & remain relatively flexible but although a bundle of fibres gives
good support it does result in a rather large block face.

The bundle of fibres is soaked in water (to hydrate them), dipped in 40% PVP
with 10% glycerol (this embedment medium was found to be most compatible
with wool fibres in terms of hardness & ease of cutting) & frozen in a
hexane/petroleum spirit slush (found to be most suitable).

The problem(s) is that the wool fibres tend to compress on cutting so that
for some of the cutting cycles no wool sections are obtained & then some
very thick sections are cut. Occasionally, a thinner section will be cut
which is OK for STEM (but then there are other problems: folding, no cuticle
on fibre...).

Secondly, & perhaps more easily solvable (?), due to the nature of the block
face (as described earlier with a fibre bundle surrounded by enough
PVP/glycerol to support the bundle) a large amount of "ice"/PVP + glycerol
sections - ie. without wool - are produced. This, in conjunction with the
compression problem, results in many small (because they are compressed)
sections overlapping or on top of one another, instead of nice cryosections
"peeling off" as occurs with "easier" tissues. All of these problems mean
that cryotransfer of these sections to the microscope results in a grid
containing alot of ice, some very thick sections & the very occasional
thinner section (& the ice usually obstructs examination of the
ultrastructure of these sections). These time-consuming procedures give a
low yield of useable sections.

I would be most grateful if anyone could pass on any useful advice,
comments, references, methods or related experiences.
Many thanks

Rachel
EM Lab, QUT
Brisbane, Australia





From: t.bostrom-at-qut.edu.au (Thor Bostrom)
Date: Thu, 07 Sep 1995 20:37:18 +1000
Subject: Re: cryosectioning wool

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In response to Rachel's difficulties with cryosectioning wool fibres, I have
a few comments which I thought I would throw to the net also to stimulate
some ideas.

1. It's important that the specimen is firmly held and does not flex during
sectioning, therefore it should be mounted so it doesn't project far out
from the chuck.
2. A cryo diamond knife might help to give more reproducible sectioning.
3. In past experience I've found that some stubborn materials sometimes cut
better with a lower angle glass knife, ie. a 40 or 35 degree knife, though
the knife edge probably doesn't last as long as a 45deg one.
4. Freezing in liquid propane might be better, though it probably would not
solve the primary problem that the fibres remain flexible at low temperature.
5. Try a very low sectioning speed, or section manually so you can control
it. If it doesn't help, very occasionally a fast speed might work instead.
6. Is it possible to mount the fibre bundle in a thin tube made of some
material which could be sectioned together with the fibres?
7. It's often difficult with non-cryoprotected specimens to get nice regular
sections, and collecting sections with an eyelash probe can be fiddly, so
productivity is usually fairly low anyway. If collection of the sections is
an additional problem, perhaps an anti-static device might help.
8. Rather than using the sections, the frozen sectioned fibre bundle could
be mounted on a cold stage in a cryo-SEM, and the cut ends examined and
analysed directly. Unfortunately we do not have such a facility in Brisbane,
but perhaps arrangements could be made with another lab.

Any other ideas anyone?
Regards
Thor
----------------------------
Dr Thor Bostrom
Analytical EM Facility
Queensland University of Technology (QUT)
GPO Box 2434, Brisbane, QLD 4001, Australia
Ph: (61) 7-3864-2351 FAX: (61) 7-3864-5100






From: hawkey-at-neuro.duke.edu (Larry Hawkey)
Date: Thu, 7 Sep 1995 07:57:38 -0500
Subject: Job related injury

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Message-Id: {v01510102ac7499e80803-at-[152.3.72.63]}
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In response to John A.
I have had bursitis in both shoulder for years but I doubt that it is job
related. It has been with me for to long. I do however known many EM'ers
who have had sinus problems. Mine have gotten worse over the years. I
have been doing EM for about 14.5 years.

} From JOHNA-at-SCI.WFEB.EDU Wed Sep 6 16:23:32 1995

} Hello folks,

} I have just undergone heavy duty surgery on my right shoulder: tremendous
} arthritis, bone spurrs, etc. etc. We did the right shouder because I am
} right handed and because it was much worse than the left which will
} probably need to be done in a year or two. Someone just said to me that
} all electron microscopists end up with shoulder problems. I had not heard
} this before. I've been doing EM full time for well over twenty years so I
} guess I qualify as a "lifer". Have any of you EMers out there ever heard
} of anything like this or have you experienced similar proble pro I'm just
} curious; if it's true we should warn new EMers and perhaps get the
} manufacturers to institute design changes to ameliorate such problems.

} Any thoughts?

} John A.






From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-aaem.amc.anl.gov
Date: Thu, 7 Sep 1995 8:32:15 -0500 (CDT)
Subject: Help finding a Company

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Colleagues,

The following message was sent to the WWW Microscopy & Microanalysis
site can anyone help this person?

If you have the information reply to the address at the end of
the message (Email: pj8v-at-nih.gov) . Do not use Email reply as the
message will go to me not the originator.

Nestor

=================




From: Peter Jackson, PhD
Date: Wed, 6 Sep 1995 13:30:06 -0600
Subject: Macarthur or Mcarthur Microscopes - information

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X-Sender: www-at-aaem.amc.anl.gov (Unverified)
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"


I would like to know, if by any chance you might have the information,
the address/phone of MacArthur or McArthur Microscores of Cambridge, England.
The company used to make a small hand held light microscope that was used
in the
field, especially by scientists conducting work on parasitic diseases.
Would you happen to know the address/phone or someone who might?
I am trying to get the info to a missionary trying to do malaria
diagnosis in Hati under primative conditions. No electricity so
a self contained high power (oil immersion to high dry) scope is needed.

Thanks,

Peter Jackson, Ph.D.
fax 301-402-2638
Ph 301-496-8426
Email: PJ8V-at-NIH.GOV

..







From: William Tivol :      tivol-at-wadsworth.org
Date: Thu, 7 Sep 1995 11:57:47 -0400 (EDT)
Subject: Re: Need Help with Cryosectioning

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Rachel Garlick asks about wool cryosectioning.

Dear Rachel,
What thickness sections are you cutting? I have no real experience
with cryosectioning (I was at a demo, and we have a Reichert), but very thin
sections are difficult in general, and your specimen seems particularly patho-
logical. Could you get away with thicker sections? Do you have access to an
IVEM? If so, that would be a possible way out. Good luck.
Yours,
Bill Tivol




From: William Tivol :      tivol-at-wadsworth.org
Date: Thu, 7 Sep 1995 12:32:40 -0400 (EDT)
Subject: Re: Solder Analysis

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John Giles writes:

} I am interested on the community input on performing in-situ analysis of solder
} to determine composition. We have tried to do this in the past and have not
} had acceptable results. We used both multiple areas and multiple points and
} averaged the data.
}
} I realize that this is probably an impossible task with EDS, but would like to
} confirm my opinion and obtain input as to any other techniques (micro probe
} XRF, etc.) which may provide a non-destructive method for in-situ solder
} analysis.

Dear John,
What size specimen do you need to analyse, and what size is the solder
blob? Is your problem related to the volatility of the solder components?
How accurate do you need the quantitation? If your specimen fits in a SEM,
you could try EDS at relatively high voltage (necessary for Pb) and low beam
current to minimize volatility problems. Possibly proton-induced x-ray emis-
sion (PIXE) will do the job. If you are interested in this technique, I'd
contact Dr. Tom Cahill at UC Davis. He has examined everything from air
pollution to a page from a Guttenberg Bible. I don't have his email address,
but his phone # is (916) 752-1460. Good luck.
Yours,
Bill Tivol




From: William Tivol :      tivol-at-wadsworth.org
Date: Thu, 7 Sep 1995 12:09:18 -0400 (EDT)
Subject: Re: EDS detector HV stability

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} OFFICE MEMO EDS detector HV stability Date: 9/6/95
}
} We never turn our EDS detectors off, and depend on the automatic shutdown in
} the rare occasions that the LN2 is depleted. The only question I have about
} turning them on and off routinely is whether the stability of the calibration
} is maintained after numerous shutdowns and re-starts. Anyone have any
} observations?
} Larry
}
Dear Larry,
We have checked the calibration on our TN2000 (an oldie), and we find
that it is maintained pretty well--to one or two channels, or 10 or 20 ev.
Noran has a somewhat tedious procedure for energy calibration which works well
for us with aluminum on a copper grid. This procedure takes less than 1 hr
and is accurate to better than one channel. I don't know what components are
in the circuits, but I'd worry more about drift due to decay of one of them
than due to restarts. BTW, we use our EDS only infrequently; if you use yours
daily, it's probably right to leave it on.
Yours,
Bill Tivol




From: MELSEN :      MELSEN-at-microbio.emory.edu
Date: Thu, 7 Sep 1995 15:49:56 EST
Subject: LOWICRYL

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For the first time ever, I have been asked to section some material prepared
in UV polymerized Lowicryl resin. The sample material appears to refuse
to be cut while the plastic cuts routinely. I have tried some heat cured
blocks from the same materials at the same time; these cut without any
problem, but the antigenicity is comprimised in the sample. Because these
samples are from an outside source, I do not have any control over the
preparation. Another contributing factor is ,without enormous expense and
difficulty, the material may not be replaceable.

Questions:
Is there a specific alternative flotation fluid used with the UV versus heat
cured Lowicryl?

Will the antigenicity be comprimised if the samples are heat cured after
they were UV cured?

Has anyone else had and solved this problem?

Please send any and all suggestions / recommendations gained through
experience with this resin.
melsen-at-MICROBIO.emory.edu
L.R. Melsen
Emory University
Microbiology and Immunology
3029 Rollin Research Center
Atlanta, Ga 30322
404 727 3508 FAX 404 727 3659




From: dcromey-at-CCIT.ARIZONA.EDU
Date: Thu, 07 Sep 1995 13:58:23 -0700 (MST)
Subject: Re: LOWICRYL

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Hi,
Any chance the persons that supplied you with the tissue used OsO4?
Methacrylate resins (Lowacryl is in this "family") do not like to
polymerize in the presence of OsO4. If they used OsO4, I'm afraid you
may be out of luck.

Just a guess re: your problems with sectioning.
Doug
.....................................................................
: Douglas W. Cromey, M.S. Dept. of Cell Biology & Anatomy :
: Sr. Research Specialist University of Arizona :
: (office: AHSC 4212A) P.O. Box 245044 :
: (voice: 520-626-2824) Tucson, AZ 85724-5044 USA :
: (FAX: 520-626-2097) (email: dcromey-at-ccit.arizona.edu) :
:...................................................................:





From: murphy-at-ms.sjdccd.cc.ca.us (Murphy, Judy)
Date: Thu, 07 Sep 1995 17:42:48 PST
Subject: Zeiss 902, Hitachi 570 available

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I am sending this for the EM lab in Marine Biology at Bodega Bay, CA (in the
UC, Davis system) Please contact them for further information.
Thanks

Two EMs are available, Zeiss EM 902 TEM and a Hitachi S570 SEM.
The listed prices through UC, Davis Central Stores and Receiving are
$79,000 for the Zeiss and $12,500 for the Hitachi. Bids at lower prices
will be considered if necessary. The sale will be processed by Central
Stores and Receiving at UC, Davis, but persons interested in submitting
bids may contact me at the Bodega Marine Lab.
Fred Griffin, PhD
Telephone 707/875-2045
FAX 707/875-2009
e mail: fjgriffin-at-ucdavis.edu
______________________________________________________________________________
San Joaquin Delta College World Wide Web:http://www.sjdccd.cc.ca.us
5151 Pacific Avenue email:webmaster-at-ms.sjdccd.cc.ca.us
Stockton, CA 95207
general information:(209) 474-5151 or FAX-at-(209)474-5600





From: SveEn-at-pai.liu.se (Sverker Enestrom)
Date: Fri, 8 Sep 1995 10:44:39 +0200
Subject: Re: LOWICRYL

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Fortunately, the designer of the Lowicryl family (K4 M, HM20, K11, HM23) Eric
Carlemalm can be consulted directly. I think we all will learn a lot from his
reply so I am forwarding your question to Eric.

} For the first time ever, I have been asked to section some material prepared
} in UV polymerized Lowicryl resin. The sample material appears to refuse
} to be cut while the plastic cuts routinely. I have tried some heat cured
} blocks from the same materials at the same time; these cut without any
} problem, but the antigenicity is comprimised in the sample. Because these
} samples are from an outside source, I do not have any control over the
} preparation. Another contributing factor is ,without enormous expense and
} difficulty, the material may not be replaceable.
}
} Questions:
} Is there a specific alternative flotation fluid used with the UV versus heat
} cured Lowicryl?
}
} Will the antigenicity be comprimised if the samples are heat cured after
} they were UV cured?
}
} Has anyone else had and solved this problem?
}
} Please send any and all suggestions / recommendations gained through
} experience with this resin.
} melsen-at-MICROBIO.emory.edu
} L.R. Melsen
} Emory University
} Microbiology and Immunology
} 3029 Rollin Research Center
} Atlanta, Ga 30322
} 404 727 3508 FAX 404 727 3659

*********************************************************
Sverker Enestrom M.D., Ph.D.
Department of Pathology
University of Linkoping, Sweden
Phone: +46 13 22 15 20
Fax: +46 13 13 22 57
*********************************************************






From: SveEn-at-pai.liu.se (Sverker Enestrom)
Date: Fri, 8 Sep 1995 11:03:54 +0200
Subject: Re: LOWICRYL

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Fortunately, the designer of the Lowicryl family (K4 M, HM20, K11, HM23) Eric
Carlemalm can be consulted directly. I think we all will learn a lot from his
reply so I am forwarding your question to Eric.

} For the first time ever, I have been asked to section some material prepared
} in UV polymerized Lowicryl resin. The sample material appears to refuse
} to be cut while the plastic cuts routinely. I have tried some heat cured
} blocks from the same materials at the same time; these cut without any
} problem, but the antigenicity is comprimised in the sample. Because these
} samples are from an outside source, I do not have any control over the
} preparation. Another contributing factor is ,without enormous expense and
} difficulty, the material may not be replaceable.
}
} Questions:
} Is there a specific alternative flotation fluid used with the UV versus heat
} cured Lowicryl?
}
} Will the antigenicity be comprimised if the samples are heat cured after
} they were UV cured?
}
} Has anyone else had and solved this problem?
}
} Please send any and all suggestions / recommendations gained through
} experience with this resin.
} melsen-at-MICROBIO.emory.edu
} L.R. Melsen
} Emory University
} Microbiology and Immunology
} 3029 Rollin Research Center
} Atlanta, Ga 30322
} 404 727 3508 FAX 404 727 3659

*********************************************************
Sverker Enestrom M.D., Ph.D.
Department of Pathology
University of Linkoping, Sweden
Phone: +46 13 22 15 20
Fax: +46 13 13 22 57
*********************************************************






From: A. Kent Christensen :      akc-at-umich.edu
Date: Fri, 8 Sep 1995 09:54:25 -0400 (EDT)
Subject: Re: LOWICRYL

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I'm puzzled by the idea that methacrylate resins don't polymerize
properly in the presence of OsO4. When I started EM back in the 1950s,
everyone fixed tissues in OsO4 and embedded in methacrylate resins.
That was before glutaraldehyde and Epon arrived on the scene in the early
1960s.

A. Kent Christensen, University of Michigan, {akc-at-umich.edu}

-------------------------------------

On Thu, 7 Sep 1995 dcromey-at-CCIT.ARIZONA.EDU wrote:

} Hi,
} Any chance the persons that supplied you with the tissue used OsO4?
} Methacrylate resins (Lowacryl is in this "family") do not like to
} polymerize in the presence of OsO4. If they used OsO4, I'm afraid you
} may be out of luck.
}
} Just a guess re: your problems with sectioning.
} Doug
} .....................................................................
} : Douglas W. Cromey, M.S. Dept. of Cell Biology & Anatomy :
} : Sr. Research Specialist University of Arizona :
} : (office: AHSC 4212A) P.O. Box 245044 :
} : (voice: 520-626-2824) Tucson, AZ 85724-5044 USA :
} : (FAX: 520-626-2097) (email: dcromey-at-ccit.arizona.edu) :
} :...................................................................:
}
}




From: masur-at-msvax.mssm.edu
Date: Fri, 08 Sep 1995 10:42:35 -0500
Subject: Mowiol recipe

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We ordered Mowiol powder for mounting coverslips and can't seem to find a
recipe for making it up and storing it
Please share your lab instructions
Many thanks

Sandra K. Masur Box 1183
fax: 212-289-5945 Mount Sinai School of Medicine
phone: 212-241-6544 or 0089 NY NY 10029-6574
e-mail:masur-at-msvax.mssm.edu









From: Doug Davis :      doug_davis-at-maillink.berkeley.edu
Date: 8 Sep 1995 08:51:48 -0700
Subject: Freeze Slammer for sale

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Message-ID: {n1401572559.77840-at-maillink.berkeley.edu}

Subject: Time: 4:21 PM
OFFICE MEMO Freeze Slammer for sale Date: 9/5/95

FOR SALE: One Hitek (RMC) copper block freeze slammer, can be used with LN2
or liquid helium sources; appropriate transfer lines for each included.
Extras, please inquire. Original purchase price $5860, all offers will be
considered.
Doug Davis doug_davis-at-maillink.berkeley.edu
EML, 26 Giannini Hall
University of California
Berkeley, CA 94720





From: John W Heckman :      heckman-at-pilot.msu.edu
Date: Fri, 8 Sep 1995 13:02:27 -0400 (EDT)
Subject: Acrylic resin problems

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Message-Id: {199509081702.NAA32403-at-pilot05.cl.msu.edu}

I, too, have had trouble with complete*UV* initiated of polymerization of some
samples that had been embedded in Lowacryl resins. Since I had no problems
with heat initiated polymerizations in the same tissue, I assumed the the
problem was with the reduced Os absorbing enough of the UV to inhibit
polymerization in the tissues.

A phenomenon I find more perplexing is the un (intentionally) initiated
polymerization of just some of a batch of plant samples (that were OsO4 fixed)
after final infiltration in LR white, in a lab that should have no stray UV
light in it . Any ideas on this?

John Heckman
Center for Electron Microscopy
Michigan State University






From: sdw-at-biotech.ufl.edu (Scott Whittaker)
Date: Fri, 8 Sep 1995 14:25:24 GMT
Subject: Dr. Apkarians' adderss

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A collegue (Jill Verlander) of mine needs the address of Dr.
Apkarian. If you could e-mail it to her directly as I will be on vacation
she would be grateful.
Thanks


----------------------------------------------------------------------------
----------------------------------------------------------

Scott Whittaker ph: 904-392-1295
Research Assistant fax: 904-846-0251
ICBR EM Core Lab e-mail: sdw-at-biotech.ufl.edu
University of Florida





From: dlb-at-u.Arizona.EDU (David Bentley)
Date: Fri, 8 Sep 1995 13:21:18 -0700
Subject: Re: Acrylic resin problems

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Most of the problems (and solutions) to the acrylic resins, as Dr.
Christensen alluded to, goes back to, and have been solved in the the early
days of EM with methyl/butyl methacrylate. Although we haven't discovered
all the problems with the acrylic resins and still expect to see more, at
this point, consider it to be the resin of choice in many cases for sample
embedment.
I do have several suggestions in dealing with LR white embedding
problems. The curing of all acrylic resins in an exothermic process where
heat is released. In the case of osmicated tissues, heat is absorbed by the
dark osmicated tissue, which raises the temperature, which is further
absorbed, which raises the temperature further, ect. Ultimately, this will
result in full polymerization of the plastic in the bottle prior to
embedding (you may be able to "rescue" some important tissues even after
gelling by cutting the soft plastic with an applicator stick and embedding
in fresh resin). The best solution seems to store the samples in the
refrigerator during infiltration and keep the samples in the dark until just
ready to embed and polmerize them. I have heard rumors that a fair amount
of UV light does escape the flourescent lamps, although I have nothing
substantial to back such a rumor up.
Two other tricks that seem to help with LR White:
One is to purchase it from your supplier uncatalysed, and add the
catalyst when you are ready. We divide the bottle, after adding the
catalyst, into 10 ml plastic scintillation vials, and store at -80C, taking
it out as we need it. Check with your EM supplier for availability. This
idea comes from Newman, G.R. and J. A. Hobot. Resin Microscopy and
On-section Immunocytochemistry. 1993. Springer Verlag. New York., and a
suggestion a while back on this listserver.
The other trick comes from V. Lindley in: Microscopy Research and
Technique. A new Procedure for Handling Impervious Biological Specimens.
21:355-360 (1992). After the osmicated tissue is placed in gelatin capsules
filled with resin, leave the specimens at room temperature overnight prior
to putting in the oven for cure. This has eliminated the gas bubble
formation around the samples as they are cured. (This article has many
other tricks and is well worth reading.)
I agree with your analysis that dark or highly colored samples don't
polymerize well with UV and may need subsequent heat treatment to fully
cure. 50C for 24-48 hours has seemed ok thus far, but the antigens we look
at may not be that heat sensitive.
We have been collecting the ways to mess up the acrylic resins. We
are somewhere between 50 and 100, and we have only scratched the surface.
Sorry about the length of this message.
later dlb

David Bentley
Imaging Facility, Div. of Biotechnology, ARL
The University of Arizona
Tucson, Az 85721





From: Dr P. Echlin :      pe13-at-cus.cam.ac.uk
Date: Sun, 10 Sep 1995 16:32:57 +0100 (BST)
Subject: Re: AFM: Heating/Cooling Sample Stages

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Dear Michael Mallamaci:

With reference to your request for information about heating cooling
stages. You might like to look in my book "Low Temperature Microscopy and
Analaysis" Plenum Press New York 1992. There are many primary and
secodary references to heating cooling stages.

Patrick Echlin
Director, Multi-Imaging Centre
School of Biological Sciences
University of Cambridge UKOn Fri, 8 Sep 1995, Michael
Mallamaci wrote:

} Dear Microscopists,
}
} I thought I would open this question up to a more diverse microscopy audience,
} since I received only limited responses from the SPM list.
}
} We are interested in purchasing a controlled temperature stage for an AFM. It
} doesn't necessarily have to be made for the AFM, but only needs to be
} retrofitted to operate on an existing scope. The ideal stage would cool to LN2
} temperatures, heat to around 100-200 C, and offer temperature control +/- a
} couple of degrees. We realize that this may require a couple of different
} stages, and that such heating/cooling may be hazardous to the scope (a risk we
} are willing to take). We also know that a Peltier device operates within this
} temperature range, although not to the extremes, and we would like to start our
} studies with a Peltier stage if possible.
}
} Does anyone know of any vendors which supply Peltier or any other types of
} heating/cooling stages (perhaps for an SEM) which could fit our needs?
}
} We plan to build these devices ourselves if nothing is available commercially.
} We are limited to sample stages since it will be impossible for us to put the
} entire microscope in a freezer/oven due to its size. I would appreciate hearing
} from anyone who has some practical experience with the hazards and pitfalls of
} building such sample stages.
}
} Thanks to all in advance,
}
} Mike
}
} _________________________________________________________
} Michael P. Mallamaci mallamaci-at-goodyear.com
} Goodyear Tire & Rubber Company Tel: (216) 796-7436
} D/415A Analytical Sciences Fax: (216) 796-3304
} 142 Goodyear Blvd
} Akron, OH 44305 USA
}
}




From: A. Kent Christensen :      akc-at-umich.edu
Date: Sat, 9 Sep 1995 16:23:04 -0400 (EDT)
Subject: Re: Acrylic resin problems

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I'm still puzzled. I received two direct E-mail messages about
problems in UV curing of Lowicryl for OsO4 specimens before this
additional thoughtful message arrived. I wonder if Lowicryl/LR White are
more vulnerable to UV with OsO4 specimens than ordinary methacrylate was.
When I began EM as a graduate student in 1956, we always cured
methacrylate with heat, but during the time I was a postdoc with Don
Fawcett we switched to UV curing of methacrylate because it gave better
results (the tissues in those days were fixed only with OsO4).

A. Kent Christensen, University of Michigan, {akc-at-umich.edu}

--------------------------------------

On Fri, 8 Sep 1995, David Bentley wrote:

} Most of the problems (and solutions) to the acrylic resins, as Dr.
} Christensen alluded to, goes back to, and have been solved in the the early
} days of EM with methyl/butyl methacrylate. Although we haven't discovered
} all the problems with the acrylic resins and still expect to see more, at
} this point, consider it to be the resin of choice in many cases for sample
} embedment.
} I do have several suggestions in dealing with LR white embedding
} problems. The curing of all acrylic resins in an exothermic process where
} heat is released. In the case of osmicated tissues, heat is absorbed by the
} dark osmicated tissue, which raises the temperature, which is further
} absorbed, which raises the temperature further, ect. Ultimately, this will
} result in full polymerization of the plastic in the bottle prior to
} embedding (you may be able to "rescue" some important tissues even after
} gelling by cutting the soft plastic with an applicator stick and embedding
} in fresh resin). The best solution seems to store the samples in the
} refrigerator during infiltration and keep the samples in the dark until just
} ready to embed and polmerize them. I have heard rumors that a fair amount
} of UV light does escape the flourescent lamps, although I have nothing
} substantial to back such a rumor up.
} Two other tricks that seem to help with LR White:
} One is to purchase it from your supplier uncatalysed, and add the
} catalyst when you are ready. We divide the bottle, after adding the
} catalyst, into 10 ml plastic scintillation vials, and store at -80C, taking
} it out as we need it. Check with your EM supplier for availability. This
} idea comes from Newman, G.R. and J. A. Hobot. Resin Microscopy and
} On-section Immunocytochemistry. 1993. Springer Verlag. New York., and a
} suggestion a while back on this listserver.
} The other trick comes from V. Lindley in: Microscopy Research and
} Technique. A new Procedure for Handling Impervious Biological Specimens.
} 21:355-360 (1992). After the osmicated tissue is placed in gelatin capsules
} filled with resin, leave the specimens at room temperature overnight prior
} to putting in the oven for cure. This has eliminated the gas bubble
} formation around the samples as they are cured. (This article has many
} other tricks and is well worth reading.)
} I agree with your analysis that dark or highly colored samples don't
} polymerize well with UV and may need subsequent heat treatment to fully
} cure. 50C for 24-48 hours has seemed ok thus far, but the antigens we look
} at may not be that heat sensitive.
} We have been collecting the ways to mess up the acrylic resins. We
} are somewhere between 50 and 100, and we have only scratched the surface.
} Sorry about the length of this message.
} later dlb
}
} David Bentley
} Imaging Facility, Div. of Biotechnology, ARL
} The University of Arizona
} Tucson, Az 85721
}
}




From: Marcelle A Gillott :      magem-at-csd.uwm.edu
Date: Mon, 11 Sep 1995 09:07:57 -0500 (CDT)
Subject: Re: SEM mounting

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On 3 Sep 1995, Doug Davis wrote:

} Subject: Time: 11:54 AM
} OFFICE MEMO SEM mounting Date: 9/3/95
}
} Microscopy subscribers-
} I understand Duco brand cement is sometimes used for mounting specimens for
} SEM imaging.
} Anyone have experience using this? I would like to know what its general
} properties are- Cure time, outgassing, volatility under the beam, etc. Thanks
} all.
} doug_davis-at-maillink.berkeley.edu
} EML, UC Berkeley
}
I have used Duco cement for mounting large specimens:

- place a small drop of Duco cement on the stub
- place a small drop of Tube Koat (or any other conductive glue) next
to it on the stub
- mix well with a toothpick
- apply the sample
- dry at least overnight before coating or putting in scope

- I have not attempted to determine outgassing times, etc but this seems
to work for me
- I just put the sample in a fume hood and cover partially -

- adding the tube koat makes the adhesive layer conductive
- this works really well for large samples,
- if the surface in contact with the stub is very uneven you can use a
little more cement to fill in the gaps -

I do not have any reference for this but it is my impression that this
is a commonly usd technique


Marcelle Gillott
UWM




From: dcromey-at-CCIT.ARIZONA.EDU
Date: Mon, 11 Sep 1995 08:09:37 -0700 (MST)
Subject: Re: Acrylic resin problems

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{Pine.SOL.3.91.950909161447.11231B-100000-at-breakout.rs.itd.umich.edu}
To: "A. Kent Christensen" {akc-at-umich.edu}
Cc: David Bentley {dlb-at-u.Arizona.EDU} , Microscopy-at-aaem.amc.anl.gov
Message-id: {Pine.PMDF.3.91.950911074544.541071992B-100000-at-CCIT.ARIZONA.EDU}
X-Envelope-to: Microscopy-at-aaem.amc.anl.gov
MIME-version: 1.0
Content-type: TEXT/PLAIN; charset=US-ASCII
Content-transfer-encoding: 7BIT

Kent,
I don't know what to say about your OsO4 experience with Lowacryl and
Methacrylates. I doubt it's because Dave Bentley and I are both from
Arizona (we can cure epoxy outdoors this time of year, "but it's a dry
heat" ;-)). Seriously, my experience with Methacrylates is limited, but
here's what I remember: JB-4 refused to heat polymerize around pieces of
glut/OsO4 fixed nerve tissue (a real mess) and I believe the instructions
hinted that this could be a problem. Lowacryl (I wish I still had a copy
of the package insert) mentioned the OsO4 problem, particularly with UV
polymerization (poorly polymerized tissue in the center of the block,
this can be a problem with too large pieces of pigmented tissues eg liver
that have never seen OsO4). LR White, I have a package insert from EM
Corp that states "Osmium Tetroxide reacted tissues should not be 'cold
cured' with the accelerator. This process is strongly exothermic and
the dark colour of the tissue leads to focal heat accumulation which can
cause local problems in and around the tissue."

This is not to cast doubt on the veracity of your experiences. We all
know that there is as much "art" as there is "science" in doing EM.

Have a good week.
Doug

.....................................................................
: Douglas W. Cromey, M.S. Dept. of Cell Biology & Anatomy :
: Sr. Research Specialist University of Arizona :
: (office: AHSC 4212A) P.O. Box 245044 :
: (voice: 520-626-2824) Tucson, AZ 85724-5044 USA :
: (FAX: 520-626-2097) (email: dcromey-at-ccit.arizona.edu) :
:...................................................................:





From: BOB ROBERTS :      ROBERTS-at-CSSS.LA.ASU.EDU
Date: Mon, 11 Sep 1995 09:40:18 -0700 (MST)
Subject: Philips EM300

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Recently I sent a message to the listserver offering a EM300 for sale. The
instrument has been sold. Thanks to those who requested donations; there
were some interesting prospects for the donation list.
Bob Roberts
Arizona State University
Center for Solid State Science




From: GREG VAUGHN MARTIN :      gmartin-at-welchlink.welch.jhu.edu
Date: Tue, 12 Sep 1995 11:03:33 -0400 (EDT)
Subject: cryomicrotomy

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Message-Id: {199509121343.IAA12973-at-belize.ucs.indiana.edu}

Hey folks --
Is anybody out there looking to sell a (hopefully) low-milage
Reichert FC4E cryo attachment? Our FC4 (a museum piece, but used on a
Reichert Ultracut E) is on it's last legs and we would like to avoid
having to buy BOTH a new microtome and cryo unit. Please reply to me
personally at gmartin-at-welchlink.welch.jhu.edu
Thanks in advance,

Greg Martin
Dept. of Cell Biology and Anatomy
Johns Hopkins School of Medicine




From: jeolbxl-at-ub4b.eunet.be (JEOL Brussel)
Date: Tue, 12 Sep 1995 17:34:07 +0200
Subject: 2nd hand Polaroid Camera for JEOL JSM-T330

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To all (EX)-Jeol users,

We are looking for a 2nd hand CAMERA for a JSM-T330A. The JEOL name for
this unit is "CSI-5". This is the camera unit for polaroid photos.
If you are not using this unit anymore, we are interested in buying it from
you.

Please, let us know the price.

Thanks in advance.

Our address :

JEOL (EUROPE) BV
Service Department
Ikaroslaan 7A - 1930 Zaventem
BELGIUM
TEL : (32) 02 720.05.60
FAX : (32) 02.720.61.34








From: Jessica C.P. Chang :      changj-at-ecn.purdue.edu
Date: Tue, 12 Sep 1995 12:50:01 -0500 (EST)
Subject: Opening for post-doc on TEM

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Message-Id: {199509121750.MAA07846-at-dynamo.ecn.purdue.edu}

}
} Post-doc on TEM available - The NSF-Materials Research Group at the
} School of Electrical and Computer Engineering of Purdue University has an
} immediate opening for a post-doctoral research associate. The position
} requires hands-on experience on transmission electron microscopy (TEM)
} including electron diffraction pattern analysis, bright-field, dark-field,
} weak-beam, and high-resolution TEM, as well as specimen preparation for
} plan-view and cross-sectional TEM. The employee will be responsible for
} the characterization of molecular-beam epitaxy (MBE) grown II-VI, III-V
} and/or nitrides wide bandgap semiconductor heterostructure devices,
} including analysis of the failure mechanism of II-VI lasers and light-
} emitting diodes. Duties will also include maintenance of sample
} preparation facilities including a Gatan Dual ion mill and a Jeol-2000 EX
} high-resolution transmission electron microscope which is under service
} contract. Rate of pay will be commensurate with qualifications and
} experience. All applications should send resumes to Professor Robert L.
} Gunshor, School of Electrical and Computer Engineering, Purdue University,
} West Lafayette, IN 47907-1285; Fax: (317) 494-2706. Purdue University
} is an AA/EEO/ADA employer.
}





From: jfmjfm-at-umich.edu (John F. Mansfield)
Date: Tue, 12 Sep 1995 14:14:13 -0400
Subject: ADJUNCT LECTURER POSITION

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X-Sender: jfmjfm-at-srvr5.engin.umich.edu
Message-Id: {v02120d15ac7b7e3ad04c-at-[141.213.21.13]}
Mime-Version: 1.0
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Please do not reply to me directly, send your inquiries to Albert Yee
(afyee-at-engin.umich.edu)

ADJUNCT LECTURER POSITION

THE UNIVERSITY OF MICHIGAN

The Department of Materials Science and Engineering at The University of
Michigan, Ann Arbor, is seeking candidates for a non-tenure track lecturer.
Responsibilities include: assisting or leading in the development of new
undergraduate teaching materials and methods that place a strong emphasis
on multimedia presentation techniques; the development of self-paced,
interactive learning modules; participation in the development and teaching
of new undergraduate laboratory modules that place a strong emphasis on
processing and physical and mechanical property characterization. The
department currently enrolls approximately 140 undergraduate students and
is an integrated materials program encompassing metals, ceramics, polymers
and EMO materials. Applicants must have a Ph.D. in materials science and a
demonstrated interest in undergraduate education. Minimum one year
appointment, renewable to three years based on funding and performance.
Send resume and list of references to:

Professor Albert F. Yee, Chairman
Department of Materials Science and Engineering
The University of Michigan
2300 Hayward Street
Ann Arbor, MI 48109-2136
or
afyee-at-engin.umich.edu

An Equal Opportunity Affirmative Action Employer



John Mansfield
North Campus Electron Microbeam Analysis Laboratory
413 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (313)936-3352 FAX (313)936-3352
jfmjfm-at-engin.umich.edu, John.F.Mansfield-at-umich.edu
or jfmjfm-at-umich.edu they all reach me!
URL: http://www.engin.umich.edu/~jfmjfm/jfmjfm.html






From: vickie-at-macc.wisc.edu
Date: Tue, 12 Sep 1995 13:11:10 -0600
Subject: Symposium Announcement

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Message-Id: {25091213104258-at-vms2.macc.wisc.edu}

Symposium on:

"Integrated Microscopy"

September 29 to October 1, 1995

Organized by:

Integrated Microscopy Resource (IMR)
a Biomedical Research Resource

at:

The Wisconsin Center
702 Langdon Street
Madison, WI 53706


Progran Schedule and Abstracts can be found on the IMR Web page:

http://www.bocklabs.wisc.edu/imr/imr.html




#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*
Presentations will focus on biological problems for which a combination of
microscopies [i.e. integrated microscopy] has been used. The speakers will
demonstrate by example the power, potential and limitations of various
microscopical techniques. The techniques which will be discussed include:
DIC, Confocal, 2-Photon Excitation Imaging, SEM, TEM, Cryo-specimen
Preparation, and AFM.
#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*


FEES:
General Registration $100.00 (On Site: $130.00)
(Includes: Opening Reception, Social and Buffet Dinner, Coffee
Breaks and Materials)

Local Registration $ 80.00 (On Site: $110.00)
(Includes: Opening Reception, Social, Coffee Breaks and

Materials)




TO RECEIVE A BROCHURE AND REGISTRATION FORM
SEND COMPLETE MAILING INFORMATION TO:

IMR
Univ. Wisconsin-Madison
1675 Observatory Drive
Madison, WI 53706

OR EMAIL: imradmin-at-calshp.cals.wisc.edu





From: MLIBERA-at-VAXC.STEVENS-TECH.EDU
Date: Tue, 12 Sep 1995 16:06:57 -0500 (EST)
Subject: post-doc opening

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Post Doctoral Position
Polymer Microscopy

There is an opening at the Stevens
Institute of Technology for a post
doctoral researcher with interest and
expertise in the area of electron
microscopy of polymers. The optimal
applicant will be fluent in the
techniques of cryo-ultramicrotomy,
heavy-element staining, and
traditional diffraction-contrast TEM
imaging techniques. He/she must have
excellent communication skills.
Experience with energy-loss and x-ray
spectroscopies will be helpful. The
successful applicant will work
collaboratively with both graduate
students and industrial researchers
using a 300keV TEM, a 200keV FEG
TEM/STEM, and a digital FEG SEM among
other tools. The microstructural
problems of interest will be related
primarily though not exclusively to
structure/processing relations in
compatibilized homopolymer blends. A
one-year renewable appointment is
anticipated to begin in January 1996.
Applicants should submit their
resume, publication list, copies of
selected publications, and the names
of three references to:

Prof. M. Libera
Dept. of Materials Science
and Engineering
Stevens Institute of Technology
Hoboken, New Jersey 07030






From: Williams Keith - Exp. Biology :      KWILLIAM-at-eagle.mrc.ac.za
Date: Wed, 13 Sep 1995 10:03:22 GMT-0200
Subject: Letraset type fonts

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Message-ID: {MAILQUEUE-101.950913100322.416-at-eagle.mrc.ac.za}

Hi there fellow microscopists

We digitise some of our electron micrographs using a flat bed
scanner. We are looking for a possible site for downloading Ted Pella
letraset type fonts. The type we looking for should preferably be
black characters on a white background. Information concerning any
sources will be welcome.

Thanks
********************************
Keith Williams
Experimental Biology Programme
Medical Research Council
Tygerberg, SA

email: kwilliam-at-eagle.mrc.ac.za
*********************************




From: STEELE-at-KRDC.INT.ALCAN.CA
Date: Thu, 07 Sep 1995 17:22:05 -0500 (EST)
Subject: Re: Job related injury

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--Boundary (ID sL9J3/G6+sQD75uBz4KiOg)
Content-type: TEXT/PLAIN

EM related injuries? Well, there's failing eyesight from too many day's
spent straining to see in that dark room (Mom always warned me), and this
curious dent in my forehead from leaning it against the column whilst trying
to peer in the window. And then there's this nasty pain in my neck which
goes away as soon as my clients do.

Sorry to make light of this, actually my physio therapist tells me that the
classic bent-over-arms-out TEM position is a likely contributor to my own
neck and shoulder problems (that and age). Maybe the mouse driven scopes of
the future will spare microscopists to come.

--Boundary (ID sL9J3/G6+sQD75uBz4KiOg)--




From: Peter Steele :      70152.3105-at-compuserve.com
Date: 13 Sep 95 09:58:29 EDT
Subject: Re: Letraset type fonts

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Why not use any of the desktop or graphics software (e.g., Corel) to make your
own set of fonts, symbols or arrows for labelling digitized copy? Almost all of
the various packages today allow importing a wide variety of image formats and
would provide the maximum in flexibility as to size and font.





From: tphillips-at-biosci.mbp.missouri.edu (Tom Phillips)
Date: Wed, 13 Sep 1995 09:20:42 -0600
Subject: Re: Letraset type fonts

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Message-Id: {v01510100ac7ca63bcd19-at-[128.206.15.189]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

You can do this in PhotoShop. Place a white or black letter on your image
using the standard type tool. Leave it selected so that the marching ants
still outline it. Switch the foreground/background colors so now you have
black instead of white or vice versa. Under the Edit pull down menu there
is a selection called Stroke. Select stroke and a dialog box comes up. We
typically are working with text being placed on the image at 324 dpi so we
set the pixel option to 3 and select the outside option. this will place a
black border around a white letter similar to the old letraset method.
good luck.




} Hi there fellow microscopists
}
} We digitise some of our electron micrographs using a flat bed
} scanner. We are looking for a possible site for downloading Ted Pella
} letraset type fonts. The type we looking for should preferably be
} black characters on a white background. Information concerning any
} sources will be welcome.
}
} Thanks
} ********************************
} Keith Williams
} Experimental Biology Programme
} Medical Research Council
} Tygerberg, SA
}
} email: kwilliam-at-eagle.mrc.ac.za
} *********************************

Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility
3 Tucker Hall
University of Missouri
Columbia, MO 65211
(314)-882-4712 (voice)
(314)-882-0123 (fax)






From: STEVEN E SLAP :      75767.640-at-compuserve.com
Date: 13 Sep 95 10:57:56 EDT
Subject: Re: Letraset type fonts

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Dear Keith-
I'm probably going to take a lot of flak for this, but it is important that our
colleagues understand what goes on in the commercial world. The black-on-white
dry lettering sheets offered by Ted Pella, or Energy Beam Sciences or anyone
other EM supplier are not "Letraset type fonts." That is, they are not sheets
that Letraset or any other graphic arts company has designed to sell in art
supply shops, with an EM suppliers name on it. They are custom sheets, designed
and paid for by the EM supplier. In the case of black-on-white sheets, this
involves two printing plates.
Most of the existing dry lettering sheets were developed by EM supply companies
at the request of customers. In some cases, the demand for that particular
sheet never paid back the cost of the artwork and plates, but we do these things
because our customers are our best resource for new ideas.
Ted Pella uses a modified Helvetica font for his sheets; we use a modified
Futura font. But the artwork involved in the creation of these sheets is our
intellectual property, just as if it were copyrighted. The notion that someone
would consider "downloading" this art without a license from the company that
owns the design makes me sad.
Thanks for letting me vent!
Steven Slap





From: emccjb-at-ttuhsc.edu (Charles J. Butterick)
Date: 13 Sep 95 10:57:56 EDT
Subject: Fonts

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To: MICROSCOPY-at-AAEM.AMC.ANL.GOV

Greetings,
Steve Slap is correct. Downloading or copying fonts without a
license is inappropriate.
However, what you want is achievable at little cost and some
effort. Font disks are fairly inexpensive to buy. That gives you the
license to use a font for the intended purpose, i.e. typing (better yet,
just use whatever fonts you have, Helvetica is very common). The only
thing you really want is a white bacground so the alphanumerics will stand
out on a micrograph. Make the text bold and shadow your font with white
(this will provide a form fitting look) or put the alphanumerics on a white
background. I know you can do this in Photoshop and suspect that any
number of other graphics programs can do the same.
One can always create an acceptable substitute to dry transfer
lettering by using a laser printer. I just used MS Word with a bold,
outlined and a bold shadowed script to print white text/lines on a black
background on Labelon Laserdraft Film. What you do is create whatever
alphanumerics you need. Print it on this clear, sticky-backed material
through a standard laser printer, cut it to size, pull off the backing and
put it on any micrograph you want.
Or you can always buy what you need from our vendors.....



Charles J. Butterick (Chuck)
Electron Microscopy Center
Department of Cell Biology
and Biochemistry
Texas Tech University Health
Sciences Center
3601 4th Street
Lubbock, Texas 79430

vox (806) 743-1633
fax (806) 743-1219
email emccjb-at-ttuhsc.edu or
chuck-at-micron1.lubb.ttuhsc.edu






From: AWBlackwoo-at-aol.com
Date: Wed, 13 Sep 1995 15:11:06 -0400
Subject: Type Fonts

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13 September 1995

At the risk of offending virtually all of you, I feel the need to wade into
this discussion.

I work for Structure Probe, Inc./SPI Supplies, which is a major supplier of
customized transfer sheets for the benefit of microscopists, specifically
electron microscopists and other laboratory types, among many other things.

My daughter works for an outfit called Font Haus, which is in the business of
selling fonts to people who use them AND LICENSING THEIR USE!

The use of this bulletin board to request the assistance of fellow
microscopists in stealing the intellectual property of my esteemed
competitor, Ted Pella, is something that I find personally offensive.

If you need any of the (literally) tens of thousands of fonts that Font Haus
sells, along with an appropriate license for your intended use, please call
them at 1 800 942 9110. They will be happy to lead you through the process
of selecting and licensing a font. They do not, however, sell transfer
sheets.

I don't normally comment much on this BB, but the use of it to propose to
commit the theft of intellectual property is an indication of the extent to
which the moral climate of our community has deteriorated, and it sickens me.

Andy

Andrew W. Blackwood, Ph.D.
Structure Probe, Inc.
63 Unquowa Road
Fairfield, CT 06430-5015
Ph: 1 203 254 0000
FAX: 1 203 254 0000
e-mail: AWBlackwoo-at-aol.com
WWW: http://mail.cccbi.chester.pa.us/spi/spihome.html






From: tphillips-at-biosci.mbp.missouri.edu (Tom Phillips)
Date: Wed, 13 Sep 1995 16:03:24 -0600
Subject: Re: Type Fonts

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Message-Id: {Chameleon.950913164202.tonygr-at-emlab.mit.edu}

I think the vendors are getting in a snit for nothing concerning these
fonts. I read the original request to simply mean the individual was
trying to achieve digitally a font which was black with a white border or
white with a black border similar to what Ted Pella sells. Obviously
scanning in Pella's fonts would be a copyright violation but the individual
obviously wasn't interested in this since he owns a scanner and wasn't
doing it. Even if one wanted to violate Pella's copyrights, it wouldn't be
worth it. The resulting images would be lower quality compared to the
original, not Postscript scalable and be filled in and not just outlines.
Surely Pella and the other vendors aren't suggesting Pella owns the
copyright on the concept of placing contrasting borders of fonts. One can
buy such fonts from dealers as has been suggested but a simpler and cheaper
and fully legal way is to create them in Photoshop or an equivalent
program. To do it in Photoshop: Place a white or black letter on your
image using the standard type tool. Leave it selected so that the marching
ants still outline it. Switch the foreground/background colors so now you
have black instead of white or vice versa. Under the Edit pull down menu
there is a selection called Stroke. Select stroke and a dialog box comes
up. We typically are working with text being placed on the image at 324
dpi so we set the pixel option to 3 and select the outside option. this
will place a black border around a white letter similar to the old letraset
method. Does any vendor think this is an infringement of Pella or
Letraset? If so, they should talk to Adobe and a lot of other graphic
programs.

Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility
3 Tucker Hall
University of Missouri
Columbia, MO 65211
(314)-882-4712 (voice)
(314)-882-0123 (fax)






From: Steve Barlow :      sbarlow-at-sunstroke.sdsu.edu
Date: Wed, 13 Sep 1995 16:46:32 -0700
Subject: LM-follicle stains

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Message-Id: {Chameleon.950913183458.tonygr-at-emlab.mit.edu}


This question was forwarded from the Center for Reproduction of Endangered
species here in San Diego.
Please respond to sbarlow-at-sunstroke.sdsu.edu:

We are trying to differentiate between healthy and atretic follicles in
dog ovaries and have run into problems when we shift from whole tissue to
isolated preparations. In paraffin embedded tissue sections, follicles
can be unequivocally differentiated using the Shorr stain (contains
Biebrich Scarlet, Orange G, Fast Green, PTA, Phosphomolybdic acid, acetic
acid in 50% alcohol). In healthy follicles, zona pellucida stain green ,
whereas atretic zona pellucida stain orange.
In isolated follicles, this differentiation is not observed between
known healthy vs atretic follicles. To approximate the treatment the
paraffin embeded tissue undergoes, isolated follicle preparations were
fixed, then treated to a dehydration series similar to paraffin embedding
then hydrated and stained.

Can anyone offer
1) any explanation for the apparent lack of differentiation in isolated vs
embedded, deparaffinized material and/or
2) an alternative protocol or stain that would enable us to discriminate
between healthy and atretic follicles?

Thanks in advance for your assistance

B. Durrant ( please respond to sbarlow-at-sunstroke.sdsu.edu)
----------------------------------------------------------
Dr. Steven Barlow
EM Facility/Biology Dept.
San Diego State University
San Diego CA 92182-0057
phone: (619) 594-4523
fax: (619) 594-5676
email to sbarlow-at-sunstroke.sdsu.edu





From: krogers-at-ecn.purdue.edu (kirk rogers)
Date: Wed, 13 Sep 1995 22:28:32 -0500
Subject: Re: Electropolishing high speed steel

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} A student wants to electropolish a 1cm square of M2 high speed steel -
} he says this contains Mo, W and C. I would be grateful for a
} solution composition, temperature and voltage suggestion.
} Thanks in advance.
} Mike
}
}
} Dr MJ Witcomb
} Electron Microscope Unit
} University of the Witwatersrand


I don't know if you've recieved a response or not, but Bernie Kestel's
book on electropolishing (still available from Argonne National Labs, I think
- at least in the US) suggests the use of 10% HClO4 in ethanol at 5 degrees C
and a current of 500 mA (voltage as required). This etch is for a Mo-Si
stainless steel but it may be worth a try.

-Kirk Rogers

Kirk A. Rogers
krogers-at-materials.ecn.purdue.edu
317-494-8751 office http://materials.ecn.purdue.edu/~krogers/
(coming soon)
317-494-1204 fax
Purdue University, School of Materials Engineering,
1289 MSEE building, W. Lafayette, IN 47907-1289







From: D.Wild-at-mirinz.org.nz
Date: Thu, 14 Sep 1995 16:25 +1200
Subject: TEM of bone

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I need to do some TEM on archeological bone to look at collagen fibres
-is there any tips.




From: masur-at-msvax.mssm.edu
Date: Wed, 13 Sep 1995 19:39:06 -0500
Subject: Presidential Symposium of NY Society of Experimental Microscopists

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New York Society of Experimental Microscopists Presidential Symposium
Friday, September 22, 1995
Stern Auditorium
Annenberg Building, 100th Street between Madison and 5th Ave
The Mount Sinai School of Medicine

in honor of the memory of Eric Holtzman
CELLS AND ORGANELLES

9:00 REGISTRATION

9:30 WELCOME
Dr. David R. Colman, NYSEM president 1994-1995
Dr. Sandra K. Masur, NYSEM president 1995-1996

9:40 Dr. Ursula W. Goodenough, Washington University
The evolution of sex at a cellular level

10:30 Dr. Pamela Cowin, New York University School of Medicine
Cadherin-mediated cell adhesion

11:20 Dr. James E. Rothman, Memorial Sloan Kettering Cancer Center
Machinery and mechanisms of intracellular protein transport

12:10 LUNCH

2:00 Dr. Kathryn E. Howell, University of Colorado School of Medicine
New insights into the structure and function
of the trans-Golgi network

2:50 Dr. David D. Sabatini, New York University School of Medicine
The generation of transport vesicles in the trans-Golgi region

3:40 Dr. Paul B. Lazarow, Mount Sinai School of Medicine
Peroxisomes in yeast and man

4:30 Dr. Lorna Role, Columbia University College of Physicians & Surgeons
Development of synaptic transmission between neurons

5:20 RECEPTION

Co-sponsored by The Mount Sinai School of Medicine

For additional information:

Best regards,

Sandra K. Masur Box 1183
fax: 212-289-5945 Mount Sinai School of Medicine
phone: 212-241-6544 or 0089 NY NY 10029-6574
e-mail:masur-at-msvax.mssm.edu









From: Computer Man :      dkristen-at-hobbs.leesummit.k12.mo.us
Date: Thu, 14 Sep 1995 07:27:32 -0500 (CDT)
Subject: Presidential Symposium of NY Society of Experimental Microscopists

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My name is David Kristensen, and I am trying to do a project for the
Westinghouse Talent Search. I am trying to find the structure of a virus
by looking at an electron micrograph. I will do it using Fourier
space, so I only need one of them. I would need a small virus
(preferably a polio-virus or one of its relatives) in order to be able to
find out the structure (a computer might not be able to handle a large
virus without parallel processing). So what I'm asking is, can anyone
give me/tell me where to find an electron micrograph of a small virus?

My name is David Kristensen: e-mail: dkristen-at-hobbs.leesummit.k12.mo.us
The MIND Conquers ALL.




From: rutledge phil :      prutle1-at-gl.umbc.edu
Date: Thu, 14 Sep 1995 10:41:31 -0400 (EDT)
Subject: x-ray detector

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I was wondering if anyone has used or is using an x-ray detector by
Amptek. The physics dept. here at UMBC wants to put a model number
XR-100T x-ray detector onto my JEOL JSM-35CF SEM. What I need to know
is, what kind of problems could I encounter having this unit installed on
my SEM. It is basically a low res detector (200eV) so I don't think it
would be too good for biological purposes. It is also of the dry type of
detector system that doesn't need liquid nitrogen. Any info about this
system would be greatly appreciated.

Thanks,

Phil Rutledge 8-{)
Director, Center for EM
UMBC




From: George.C.Ruben-at-Dartmouth.EDU (George C. Ruben)
Date: 14 Sep 95 10:45:32 EDT
Subject: Re:

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Message-id: {3860097-at-prancer.Dartmouth.EDU}

David,

You can not obtain a viruse's structure with just any
electron microscope image of a virus using fourier transform processing --- If
a transmission electron microscopy image is taken of a virus in vitreous ice
and a number of tilt views of this virus are also taken you may be able to
reconstruct the virus to about ~30A resolution. You could not reconstruct the
viruses internal structure with a surface replicated virus with any of the
various metals. It is also unlikely that the internal viral structure is
preserved in a negatively stained preparation of viruses for transmission
electron microscopy. By taking just a single image the sample is heated in the
electron beam to 100-200deg.C and many of the structural proteins are
incinerated in the vacuum of the microscope. This does not happen as readily
when a sample is embedded in vitreous ice at -196deg.C.

Good luck on your project,
George C. Ruben
Dept Biological Sciences
Dartmouth College
Hanover, NH




From: Maria do Carmo Goncalves :      maria-at-IQM.Unicamp.BR
Date: Thu, 14 Sep 1995 13:06:29 -0300 (EST)
Subject: subscribe

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Subscribe maria-at-iqm.unicamp.br





From: :      MELLIOTT-at-prl.pulmonary.ubc.ca
Date: Thu, 14 Sep 1995 14:13:17 +0800PST
Subject: sections sticking to slides for immuno

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Hello there. Someone in my lab is having a problem which I believe
has been covered before, but am not sure. Any help would be
appreciated. The person is forcing neutrophils through a
polycarbonate filter, fixing the filter, embedding the filter in
agarose and then into paraffin so that are cutting the filter on end
to see the cells passing through the filter. The cells look fine
when an H&E is done but when they are attempt to do an immunolabel
for Brdu, the cells and membrane are gone. We routinely use silane
coated slides for immunno without any problems until now. The
staining procedure requires a pepsin pretreatment (pH 2.5) followed
by an incubation in 2N HCl. Is there something else we could use to
attach the cells to the slides??? Would poly-L-lysine be affected by
these conditions?? We feel that the use of albumin would not work
because of the pepsin treatment. Any information/suggestions would
be greatly appreciated.
Thanx

Mark Elliott, PhD
UBC-Pulmonary Research Lab
Vancouver BC
Canada
acidic incubation as well. Is there something else wee could use




From: Glen Macdonald :      glenmac-at-u.washington.edu
Date: Thu, 14 Sep 1995 15:48:48 -0700 (PDT)
Subject: Re: sections sticking to slides for immuno

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X-Sender: glenmac-at-homer01.u.washington.edu

Mark,
How are you cleaning your slides? Chromic acid or 1% HCl in 95%
ethanol are the two methods that work the best for us, regardless of the
subbing agent used on the slides. Poly-L-lysine will survive proteases
and the 2N HCl pretreatments for BrdU, as will chrome alum-gelatin.

Regards,
Glen MacDonald
Hearing Development Laboratories
University of Washington
Seattle, WA 98195-6515
(206)543-8360
glenmac-at-u.washington.edu






From: Fred Hayes :      fahayes-at-ucdavis.edu
Date: Thu, 14 Sep 1995 23:40:13 -0700 (PDT)
Subject: Help re: TEM of polyester filter

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I have a customer who wants TEM surface characterization of polyester
filters which are "clean and dry". Pore sizes are 0.05 - 0.03 microns.
They are interested in the pore size, shape and distribution on both top
and bottom surfaces.

1) do I need to wash prior to embedding to "clean" off any manufacturing
debris?

2) will embedding in an epoxy such as EMbed-812 or Spurr's be adequate or
should I use a polyester resin?

3) should I osmicate to impart contrast and/or are there any other
contrast enhancing techniques I should use? I'm anticipating a contrast
problem since there will be no tissue (filter only).

***these filters are non elastic and are very stable on a substrated grid

all suggestions will be appreciated, many thanks.

Fred A. Hayes
Dixon, CA
916-678-6280





From: Zhiyu Wang :      zhiyu-at-hawaii.edu
Date: Thu, 14 Sep 1995 22:00:41 -1000
Subject: Re: Help re: TEM of polyester filter

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This is for Fred Hayes regarding TEM methods for polyester filters.

Hi, Hayes:

I have been working with ultrafiltration membrane materials on TEM and
SEM several years and here are some methods I have used and hopefull it
can help you.

1. Metal shadow for topography of polyester menbranes on TEM :
Just follow regular Pt/Pd metal shadow method with 10-30 degree
angle then disolve membrane material and you will get a metal replica
of the sample. This replica can be observed up to X20000 and possible to
count the holes.

2. GMA embeding and ultrasection for TEM:
Epon 812 (or its components) will disolve or damage sample. GMA
is a water soluble resin and no many components needed. I use GMA (with
1-2% PTA) for embeding and it works well. PTA will react with active layer
of the sample during embeding and show the active layer clearly. No
staining and fixation are needed. The result looks like dark field image
on TEM. Vacuum filtration with GMA embedding resin and cut membrane into
small pieces before embeding will show pores more clear since resin fill
out the holes. The pore sizing up to 100 A can be counted and measured
easily up to X50000 on film.

Hopefull it helps.

Zhiyu Wang
Department of Biosystem Engineering
University of Hawaii
Honolulu HI 96822
zhiyu-at-hawaii.edu


On Thu, 14 Sep 1995, Fred Hayes wrote:

} I have a customer who wants TEM surface characterization of polyester
} filters which are "clean and dry". Pore sizes are 0.05 - 0.03 microns.
} They are interested in the pore size, shape and distribution on both top
} and bottom surfaces.
}
} 1) do I need to wash prior to embedding to "clean" off any manufacturing
} debris?
}
} 2) will embedding in an epoxy such as EMbed-812 or Spurr's be adequate or
} should I use a polyester resin?
}
} 3) should I osmicate to impart contrast and/or are there any other
} contrast enhancing techniques I should use? I'm anticipating a contrast
} problem since there will be no tissue (filter only).
}
} ***these filters are non elastic and are very stable on a substrated grid
}
} all suggestions will be appreciated, many thanks.
}
} Fred A. Hayes
} Dixon, CA
} 916-678-6280
}
}




From: Liang, Long :      LLIANG-at-is.Arco.COM
Date: 15 Sep 1995 08:39:08 CST
Subject: EPMA standards for coal ?

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Message-Id: {MACMS.LLIANG.410035080095258FMACMS-at-IS.ARCO.COM}


Dear microscopists,

I am planning to analyze coal macerals for cabon, sulfur, and oxygen
contents using an electron microprobe. Does anyone know if there are
any microprobe standards (coal or synthetic coke) available somewhere ?

Thanks in advance.

Long Liang
ARCO EPMA/SEM Lab






From: JOY-at-utkvx.utk.edu (DAVID JOY)
Date: Fri, 15 Sep 1995 09:33:02 -0400 (EDT)
Subject: Tomography

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SOFTWARE FOR TOMOGRAPHIC RECONSTRUCTION


I am putting together a class on advanced imaging methods and one of
the subjects I want to cover is tomography. Does anyone know of any
freeware, shareware, or even a commercial program, that can demonstrate
the principles of tomographic reconstruction using any of the standard
algorithms (and appropriate input data of course). Any guidance would
be much appreciated.

David Joy "joy-at-utkvx.utk.edu"

**********************************************************************
* F239 Walters Life Sciences Bldg. NOTE NEW AREA CODE! *
* University of Tennessee Phone (423)-974-3638 *
* Knoxville, TN 37996-0810 FAX (423)-974-3642 *
**********************************************************************





From: Glenn Poirier :      GLENN_P-at-GEOSCI.Lan.McGill.CA
Date: Fri, 15 Sep 1995 11:12:09 EST5EDT
Subject: Re: EPMA standards for coal ?

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X-SMTP-Posting-Origin: lansend.cc.mcgill.ca (lansend.CC.McGill.CA [132.206.37.4])
Message-Id: {199509151522.LAA27962-at-sirocco.CC.McGill.CA}

Greetings Microscopists

Does anyone have or know of a set of database routines
suitable for cataloging microprobe standard collections? I thought
I would check before I designed my own. What I want is to be able
to search the database on different fields such as mineral type or
chemistry. Any info would be appreciated.

Glenn
**********************************************************************
* Glenn Poirier glenn_p-at-geosci.lan.mcgill.ca *
* Electron Microprobe Lab Phone: (514) 398 6774 *
* Earth and Planetary Sciences Fax: (514) 398 4680 *
* McGill University THERE ARE THREE SIDES TO EVERY STORY; *
* Montreal, Quebec YOUR SIDE MY SIDE AND THE TRUTH *
**********************************************************************




From: ychen-at-macc.wisc.edu
Date: Fri, 15 Sep 1995 12:55:36 -0500
Subject: Re: Help re: TEM of polyester filter

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Message-Id: {m0stdYm-00062VC-at-Pegasus.cc.ucf.edu}

Hayes,

The best way of imaging the surface of polyester filter is to look at them
by high-resolution SEM (low-voltage FESEM). It can view surface features
directly without complicated specimen preparation. I looked at some kind
of filter 4 years ago in the Hitachi S-900 LVSEM. The filter surface can
be imaged up to 100,000x in stereo. The 3-dimensional 5-10nm surface
details are clearly seen at this magnification. Hope this information can
help you.

Ya Chen

=========================================================================
\ / Assistant Researcher-Cryo/SEM Coordinator TEL : 608-263-8481
\ / __ Integrated Microscopy Resource (IMR) FAX : 608-265-4076
\/ / / University of Wisconsin-Madison
/ / / 1675 Observatory Drive #167 Email:YChen-at-macc.wisc.edu
/ /__/_ Madison, WI 53706 Email:chen-at-calshp.cals.wisc.edu

IMR WWW Home Pages: http://www.bocklabs.wisc.edu/imr/imr.html
=========================================================================


}
} I have a customer who wants TEM surface characterization of polyester
} filters which are "clean and dry". Pore sizes are 0.05 - 0.03 microns.
} They are interested in the pore size, shape and distribution on both top
} and bottom surfaces.
}
} 1) do I need to wash prior to embedding to "clean" off any manufacturing
} debris?
}
} 2) will embedding in an epoxy such as EMbed-812 or Spurr's be adequate or
} should I use a polyester resin?
}
} 3) should I osmicate to impart contrast and/or are there any other
} contrast enhancing techniques I should use? I'm anticipating a contrast
} problem since there will be no tissue (filter only).
}
} ***these filters are non elastic and are very stable on a substrated grid
}
} all suggestions will be appreciated, many thanks.
}
} Fred A. Hayes
} Dixon, CA
} 916-678-6280






From: m.dickson-at-unsw.edu.au (melvyn dickson)
Date: Mon, 18 Sep 1995 10:33:30
Subject: Re: Help re: TEM of polyester filter

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In article {Pine.SOL.3.91.950914232209.26887A-100000-at-chip.ucdavis.edu} Fred Hayes {fahayes-at-ucdavis.edu} writes:
} Date: Thu, 14 Sep 1995 23:40:13 -0700 (PDT)
} From: Fred Hayes {fahayes-at-ucdavis.edu}
} Subject: Help re: TEM of polyester filter

} I have a customer who wants TEM surface characterization of polyester
} filters which are "clean and dry". Pore sizes are 0.05 - 0.03 microns.
} They are interested in the pore size, shape and distribution on both top
} and bottom surfaces.

} 1) do I need to wash prior to embedding to "clean" off any manufacturing
} debris?

} 2) will embedding in an epoxy such as EMbed-812 or Spurr's be adequate or
} should I use a polyester resin?

Don't use replication or sectioning if you can help it. They CAN be used, but
they don't give an image of the real surface. For that, use the method of low
voltage field emission SEM we describe in our papers:

Kim, KJ, Dickson, M. et al (1991) Electron Microscopy in Synthetic Polymer
Membrane Research. Journal of Microscopy vol 162 pp 403-413

Kim, Dickson et al. (1992) Applications of FESEM to polymer membrane research.
Micron & Microscopica Acta. vol 23 pp 259-271.

Find someone who has an in-lens FESEM and buy their services. (We can help you
!) That will give you an immediate result much cheaper than using the other
methods you suggest.

Email me separately for other information.




From: Sally Shrom :      sally-at-retina.anatomy.upenn.edu
Date: Mon, 18 Sep 1995 10:03:51 -0400 (EDT)
Subject: subscribe

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How do I resubscribe? I haven't received mail in 2 months. I must have
been omitted. May I be readmitted?
Sally Shrom
sally-at-retina.anatomy.upenn.edu




From: richard.easingwood-at-stonebow.otago.ac.nz (Richard Easingwood)
Date: Tue, 19 Sep 1995 11:27:48 +1100
Subject: TEM:HT insulator change from freon to SF6

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Dear microscopists,
We have an Akashi EM-002A 120kV TEM which uses freon as the insulator in
the HT tank and in the gun. I am going to need to recharge the tank soon
(the pressure in it drops slowly over a year or two).
As I have used up the freon supplied with TEM I will replace it with SF6 as
recommended by Topcon/Akashi but I have heard a rumour that its not as
straightforward as simply pumping out the freon and refilling with SF6. Has
anyone out there done this type of changeover or know anything about any
potential problems?

Thanks in advance for any comments.

Richard Easingwood

Please reply to: richard.easingwood-at-stonebow.otago.ac.nz

Richard Easingwood
South Campus Electron Microscope Unit
Otago Medical School
PO Box 913
Dunedin
NEW ZEALAND

Telephone: 64-03-479 7301
Facsimile: 64-03-479 7254

The southernmost electron microscope unit in the world






From: andreas.brech-at-bio.uio.no (Andreas Brech)
Date: Sun, 17 Sep 1995 13:35:59 +0200
Subject: sectioning wood

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Hello

does anyone out there have any experience in cutting wood for transmission
electron microscopy?
Any sugestions for cryo or epon sectioning of wood?



Andreas Brech
Electron Microscopical Unit for Biological Sciences
Department of Biology, University of Oslo.
P.O.Box 1062 Blindern
N-0316 Oslo 3
Norway
Tel.: + 43-22 85 61 89 (work)
+ 43-22 43 83 23 (privat)
Fax.: + 43-22 85 47 26
e-mail.: abrech-at-bio.uio.no





From: rutledge phil :      prutle1-at-gl.umbc.edu
Date: Tue, 19 Sep 1995 10:04:29 -0400 (EDT)
Subject: confocal microscopy

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We have just purchased a Leica confocal system which will be used by the
faculty and grad students here at UMBC. I would like to build a good
library of reference books for their use and was wondering if anybody had
good suggestions as to what books would be a necessity and what books would
be nice to have. Also, does anybody know of a good security system for
the microscope so non-trained personnel won't have access to using the
scope. It would be nice if the system could be incorporated into the
software of the confocal system. Any suggestions would be greatly
appreciated.

Thanks,

Phil Rutledge, Director
Center for Electron Microscopy
University of Maryland Baltimore County
5401 Wilkens Ave.
Catonsville, MD 21228




From: kaurin-at-rmslab.rockefeller.edu
Date: Tue, 19 Sep 1995 10:20:47 EDT
Subject: tem, immunogold,collagen and cell dyes

Contents Retrieved from Microscopy Listserver Archives
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Hi! I am interested in locating a dye that will stain collagen and/or
a monolayer (huve cells) prior to embedding in LRW for postembed
immunogold labeling. This is simply to aid location of the monolayer
while facing. I have found a reference using 1%glut, 0.2% picric acid and
6mM eserine (physostigmine). Does the eserine function a dye? I intend on
using .5% glut with paraformaldehyde. Any ideas?
Shelley Landon Kaurin , kaurin-at-rmslab.rockefeller.edu






From: Doug Cromey :      dcromey-at-ccit.arizona.edu
Date: Tue, 19 Sep 1995 07:50:39 -0700
Subject: Leica confocal & security

Contents Retrieved from Microscopy Listserver Archives
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Phil,
Congratulations on your Leica confocal, its a nice instrument. We had
concerns here about security and accounting for the microscope. We are
currently using Direct Access for Windows (Symantec, which bought out 5th
Generation Systems). DAW is not perfect, but there is so little out there.
It does provide a measure of security and it collects the hours that users
are on the instrument for billing later. Another possiblity I've been
considering lately is a program called Full Armor (I've only seen it in the
TigerSoftware catalog, 800-888-4437). It seems to do most of the same
things as DAW, but it is a newer product (DAW has been in version 1.04 for
over 2 years) and it "says" it can lock file manager sub-directories
(something DAW can't do if you allow access to file manager).

Write me directly for more information. DAW was about $50 (educational
price) and Full Armor is $69.99 in the catalog. (I have no financial
interest in either program).

Doug




At 10:04 AM 9/19/95 -0400, you wrote:
}
} We have just purchased a Leica confocal system which will be used by the
} faculty and grad students here at UMBC. I would like to build a good
} library of reference books for their use and was wondering if anybody had
} good suggestions as to what books would be a necessity and what books would
} be nice to have. Also, does anybody know of a good security system for
} the microscope so non-trained personnel won't have access to using the
} scope. It would be nice if the system could be incorporated into the
} software of the confocal system. Any suggestions would be greatly
} appreciated.
}
} Thanks,
}
} Phil Rutledge, Director
} Center for Electron Microscopy
} University of Maryland Baltimore County
} 5401 Wilkens Ave.
} Catonsville, MD 21228
}
.....................................................................
: Douglas W. Cromey, M.S. Dept. of Cell Biology & Anatomy :
: Sr. Research Specialist University of Arizona :
: (office: AHSC 4212A) P.O. Box 245044 :
: (voice: 520-626-2824) Tucson, AZ 85724-5044 USA :
: (FAX: 520-626-2097) (email: dcromey-at-ccit.arizona.edu) :
:...................................................................:





From: PRING :      richard.pring-at-bbsrc.ac.uk
Date: Tue, 19 Sep 1995 16:59:38 +0000
Subject: LM photography

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Via: uk.ac.bbsrc; Tue, 19 Sep 1995 17:00:49 +0100
X400-Received: by /PRMD=UK.AC/ADMD= /C=GB/; Relayed;
Tue, 19 Sep 1995 17:02:30 +0000
X400-Received: by mta arcs.bbsrc.ac.uk in /PRMD=UK.AC/ADMD= /C=GB/; Relayed;
Tue, 19 Sep 1995 17:02:30 +0000
X400-Received: by mta LARS.MUAS in /PRMD=UK.AC/ADMD= /C=GB/; Relayed;
Tue, 19 Sep 1995 16:59:31 +0000
X400-Received: by mta LARS in /PRMD=UK.AC/ADMD= /C=GB/; Relayed;
Tue, 19 Sep 1995 16:59:33 +0000
X400-Received: by /PRMD=UK.AC/ADMD= /C=GB/; converted (ia5-text); Relayed;
Tue, 19 Sep 1995 16:59:38 +0000

I am having trouble getting the right colour balance when taking slides with a
Wild M400 photomacroscope. I am illuminating specimens with light from a
halogen bulb via a ring light or two swan necks.There is no brightness control
on the transformer, so presumably it runs at opyimum voltage ay all times.
Daylight film (Kodak
ektachrome 100) gives a yellow cast with a definite khaki/yellow to what was a
white background. Tungsten film (Ektacrome 160T) gives a green cast with a pale
green background or what should be white. I have bracketed the exposures for
both films several stops above and below the given rating. I thought that
halogen light would be approximately daylight, but it is obviously not, nor is
it tungsten - which is not surprising. Any ideas on a suitable transparency
film or fiters I can use to get correct balance?

Many thanks, Richard

Richard.Pring-at-BBSRC.ac.uk

Long Ashton Research Station, Long Ashton, Bristol, U.K.




From: DRStadden:R_D:Armstrong
Date: 9-19-95 12:17pm
Subject: Need Microtome Chuck

Contents Retrieved from Microscopy Listserver Archives
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To: MICROSCO:CENTRAL
cc: DRStadden:R_D:Armstrong
Subj: Need Microtome Chuck
------------------------------------------------------------------

Attention Sorvall rotary microtome users:

I have a need to make routine, thick sections of polymer films for cross-
sectional study by the FTIR microscope. Where can I get a chuck with
vise jaws instead of the beam capsule clamps? I'd like to simply
sandwich the three mil film of interest between polyethylene pads and
not have to embed in epoxy the way our IR guy has been doing. The unit
is of unknown vintage, but is probably at least 20 or 30 years old,
small, gray, rather unassuming. The collet itself threads onto a 5/8
mail threaded piece with a ball fitting on opposite end. Sound
familiar? You can tell I'm not a full-time microtomist. Thanks in
advance for your help.

Dave Stadden
DRStadde+aCENTRAL%ARMSTRONG-at-MCIMAIL.COM
Lancaster, PA





From: Xiaofeng Zhang :      xiaofeng_zhang-at-qmgate.anl.gov
Date: 19 Sep 1995 13:36:49 -0500
Subject: Subscription

Contents Retrieved from Microscopy Listserver Archives
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Message-Id: {199509191751.KAA08557-at-holonet.net}

Subject: Time:1:32 PM
OFFICE MEMO Subscription Date:9/19/95

Dear Sir: I would appreciate if you agree to let me join your microscopy
network. Could you please tell me how to subscribe and other requirements
from you? Thanks a lot for your kindly consideration.
Sincerely yours

Xiaofeng Zhang
MSD, ANL.
tel: 2-4764
e-mail: xiaofeng_zhang-at-qmgate.anl.gov





From: kayton-at-ohsu.edu (Robert Kayton,MAC,CROET)
Date: Tue Sep 19 11:30:54 PDT 1995
Subject: Leica confocal & security

Contents Retrieved from Microscopy Listserver Archives
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Message-Id: {m0sv7Sc-0007C8C-at-stjohns.ohsu.edu}
Message-Version: 2
} To: microscopy-at-aaem.amc.anl.gov

Phil,
Congratulations on your Leica confocal, its a nice instrument. We had
concerns here about security and accounting for the microscope. We are
currently using Direct Access for Windows (Symantec, which bought out 5th
Generation Systems). DAW is not perfect, but there is so little out there.
It does provide a measure of security and it collects the hours that users
are on the instrument for billing later. Another possiblity I've been
considering lately is a program called Full Armor (I've only seen it in the
TigerSoftware catalog, 800-888-4437). It seems to do most of the same
things as DAW, but it is a newer product (DAW has been in version 1.04 for
over 2 years) and it "says" it can lock file manager sub-directories
(something DAW can't do if you allow access to file manager).

Write me directly for more information. DAW was about $50 (educational
price) and Full Armor is $69.99 in the catalog. (I have no financial
interest in either program).

Doug




At 10:04 AM 9/19/95 -0400, you wrote:
}
} We have just purchased a Leica confocal system which will be used by the
} faculty and grad students here at UMBC. I would like to build a good
} library of reference books for their use and was wondering if anybody had
} good suggestions as to what books would be a necessity and what books would
} be nice to have. Also, does anybody know of a good security system for
} the microscope so non-trained personnel won't have access to using the
} scope. It would be nice if the system could be incorporated into the
} software of the confocal system. Any suggestions would be greatly
} appreciated.
}
} Thanks,
}
} Phil Rutledge, Director
} Center for Electron Microscopy
} University of Maryland Baltimore County
} 5401 Wilkens Ave.
} Catonsville, MD 21228
}
.....................................................................
: Douglas W. Cromey, M.S. Dept. of Cell Biology & Anatomy :
: Sr. Research Specialist University of Arizona :
: (office: AHSC 4212A) P.O. Box 245044 :
: (voice: 520-626-2824) Tucson, AZ 85724-5044 USA :
: (FAX: 520-626-2097) (email: dcromey-at-ccit.arizona.edu) :
:...................................................................:





From: kayton-at-ohsu.edu (Robert Kayton,MAC,CROET)
Date: Tue Sep 19 11:43:21 PDT 1995
Subject: confocal microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Message-Id: {m0sv7ea-0007C5C-at-stjohns.ohsu.edu}
Message-Version: 2
} To: microscopy-at-aaem.amc.anl.gov (!microscopy)


We have just purchased a Leica confocal system which will be used by the
faculty and grad students here at UMBC. I would like to build a good
library of reference books for their use and was wondering if anybody had
good suggestions as to what books would be a necessity and what books would
be nice to have. Also, does anybody know of a good security system for
the microscope so non-trained personnel won't have access to using the
scope. It would be nice if the system could be incorporated into the
software of the confocal system. Any suggestions would be greatly
appreciated.

Thanks,

Phil Rutledge, Director
Center for Electron Microscopy
University of Maryland Baltimore County
5401 Wilkens Ave.
Catonsville, MD 21228




From: kayton-at-ohsu.edu (Robert Kayton,MAC,CROET)
Date: Tue Sep 19 11:47:29 PDT 1995
Subject: Free Used Cambridge SEM Parts

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Message-Id: {m0sv7jD-0007C8C-at-stjohns.ohsu.edu}
Message-Version: 2
} To: microscopy-at-aaem.amc.anl.gov

The McCrone Research Institute in Chicago is now unloading some antique
Cambridge Stereoscan II parts by November 1 1995. Console, column, and
chamber are being retained for museum but everything else related must go.
If you are interested contact ndaerr-at-mcri.org. Thanks.




From: kayton-at-ohsu.edu (Robert Kayton,MAC,CROET)
Date: Tue Sep 19 11:48:11 PDT 1995
Subject: sectioning wood

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Message-Id: {m0sv7jF-0007C9C-at-stjohns.ohsu.edu}
Message-Version: 2
} To: microscopy-at-aaem.amc.anl.gov

Hello

does anyone out there have any experience in cutting wood for transmission
electron microscopy?
Any sugestions for cryo or epon sectioning of wood?



Andreas Brech
Electron Microscopical Unit for Biological Sciences
Department of Biology, University of Oslo.
P.O.Box 1062 Blindern
N-0316 Oslo 3
Norway
Tel.: + 43-22 85 61 89 (work)
+ 43-22 43 83 23 (privat)
Fax.: + 43-22 85 47 26
e-mail.: abrech-at-bio.uio.no





From: kayton-at-ohsu.edu (Robert Kayton,MAC,CROET)
Date: Tue Sep 19 11:48:44 PDT 1995
Subject: RE LM PHOTOGRAPY

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Message-Id: {m0sv7jH-0007CTC-at-stjohns.ohsu.edu}
Message-Version: 2
} To: MICROSCOPY-at-AAEM.AMC.ANL.GOV

Greetings,
The green cast with tungsten film (and you should be using tungsten
film) may come from a couple of sources. Remove any polarizing lenses that
may be in the light path. Thick heat resistant glass, like that found in
Olympus fluorescent add-ons to the BH-2, can also give a green cast. If
removing such things doesn't work, then try bracketing using 10cc, 20cc or
30 cc magenta filters. BTW, assuming the brightness is correct is not
wise.



Charles J. Butterick (Chuck)
Electron Microscopy Center
Department of Cell Biology
and Biochemistry
Texas Tech University Health
Sciences Center
3601 4th Street
Lubbock, Texas 79430

vox (806) 743-1633
fax (806) 743-1219
email emccjb-at-ttuhsc.edu or
chuck-at-micron1.lubb.ttuhsc.edu






From: DRStadden:R_D:Armstrong
Date: 9-19-95 12:17pm
Subject: Need Microtome Chuck

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Message-Id: {m0sv7hS-0007C5C-at-stjohns.ohsu.edu}
Message-Version: 2
} To: MICROSCOPY-at-aaem.amc.anl.gov (!MICROSCOPY)


To: MICROSCO:CENTRAL
cc: DRStadden:R_D:Armstrong
Subj: Need Microtome Chuck
------------------------------------------------------------------

Attention Sorvall rotary microtome users:

I have a need to make routine, thick sections of polymer films for cross-
sectional study by the FTIR microscope. Where can I get a chuck with
vise jaws instead of the beam capsule clamps? I'd like to simply
sandwich the three mil film of interest between polyethylene pads and
not have to embed in epoxy the way our IR guy has been doing. The unit
is of unknown vintage, but is probably at least 20 or 30 years old,
small, gray, rather unassuming. The collet itself threads onto a 5/8
mail threaded piece with a ball fitting on opposite end. Sound
familiar? You can tell I'm not a full-time microtomist. Thanks in
advance for your help.

Dave Stadden
DRStadde+aCENTRAL%ARMSTRONG-at-MCIMAIL.COM
Lancaster, PA





From: kayton-at-ohsu.edu (Robert Kayton,MAC,CROET)
Date: Tue Sep 19 11:44:39 PDT 1995
Subject: LM photography

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Message-Id: {m0sv7go-0007C8C-at-stjohns.ohsu.edu}
Message-Version: 2
} To: microscopy-at-aaem.amc.anl.gov (Non Receipt Notification Requested)

I am having trouble getting the right colour balance when taking slides with a
Wild M400 photomacroscope. I am illuminating specimens with light from a
halogen bulb via a ring light or two swan necks.There is no brightness control
on the transformer, so presumably it runs at opyimum voltage ay all times.
Daylight film (Kodak
ektachrome 100) gives a yellow cast with a definite khaki/yellow to what was a
white background. Tungsten film (Ektacrome 160T) gives a green cast with a
pale
green background or what should be white. I have bracketed the exposures for
both films several stops above and below the given rating. I thought that
halogen light would be approximately daylight, but it is obviously not, nor is
it tungsten - which is not surprising. Any ideas on a suitable transparency
film or fiters I can use to get correct balance?

Many thanks, Richard

Richard.Pring-at-BBSRC.ac.uk

Long Ashton Research Station, Long Ashton, Bristol, U.K.




From: wise-at-VAXA.CIS.UWOSH.EDU
Date: Tue, 19 Sep 1995 16:09:22 +0000
Subject: Job ad

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Just ran across an ad in the back of Science. The Wheaton (IL)
Biology Dept wants someone with experience in two or more of the following
areas: botany, zoology, electron microscopy, etc. See Science vol 269 (15
September) page 1620 for details. And good luck.

Bob


Robert R. Wise, PhD
Director, UWO Electron Microscope Facility
Department of Biology
University of Wisconsin Oshkosh
Oshkosh, WI 54901
(414) 424-3404 tel
(414) 424-1101 fax
wise-at-vaxa.cis.uwosh.edu






From: perkes-at-indirect.com (Paul R. Perkes)
Date: Tue, 19 Sep 1995 23:57:03 -0700
Subject: Optical Microscopy, Aquariums, Cameras

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Message-Id: {v01520d0bac856a445346-at-[165.247.24.34]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Fellow microscopists,

I thought someone on this list with an interest in aquariums might be
willing to give some advice to the following individual. Please respond
directly to

Steve Murrison {toolbox-at-intertrek.com}

or to this list and I will forward him your responses.

} From: Steve Murrison {toolbox-at-intertrek.com}
} X-To: editor
} Subject: (no subject)
} Organization: Living Colours - The Aquarium Shop -
} Mime-version: 1.0
} Date: Tue, 19 Sep 95 16:55:43 -0700
}
} dear editor,
} I own and operate an aquarium shop in victoria b.c.
} I have been searching for a microscope for some time now.I know very
} little about them, but I have been holding out for something fairly
} decent. I know that sounds alittle vague sorry, am interested in veiwing
} various bacterium and would also like to photograph them can you help
} me find something thanks steve.

Thanks!

Paul R. Perkes
Webmaster
Microscopy Online
webmaster-at-microscopy-online.com
Microscopy Online - http://www.microscopy-online.com/






From: Chris Gilpin :      CGILPIN-at-fs1.sem.man.ac.uk
Date: Wed, 20 Sep 1995 10:15:53 BST
Subject: tem

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Fellow microscopists,
I need to digitise some tem negatives with a view to carrying out 3D
reconstruction. If anyone is currently using a digitising device
capable of high resolution I would like to hear about it. I would be
intersted in
minimum spot size
speed of scanning
optical density range
grey levels
cost
If there is interest I will summarise the replies and post it to the
group.
Please reply to me in the first instance

Many thanks

Chris





From: Chris Gilpin :      CGILPIN-at-fs1.sem.man.ac.uk
Date: Wed, 20 Sep 1995 10:30:35 BST
Subject: TEM

Contents Retrieved from Microscopy Listserver Archives
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Fellow microscopists,
I need to digitise some tem negatives with a view to carrying out 3D
reconstruction. If anyone is currently using a digitising device
capable of high resolution I would like to hear about it. I would be
intersted in
minimum spot size
speed of scanning
optical density range
grey levels
cost
If there is interest I will summarise the replies and post it to the
group.
Please reply to me in the first instance

Many thanks

Chris

Chris Gilpin
Biological Sciences E.M. Unit
Manchester University
U.K.
0161 2755170 phone
0161 2755171 fax




From: MacisNo1-at-aol.com
Date: Tue, 19 Sep 1995 14:28:07 -0400
Subject: Confocal References

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Dr. Rutledge,

The "Bible" of Confocal Microscopy is as follows..."Handbook of Biological
Confocal Microscopy", Second Edition, Edited by James B. Pawley, Plenum
Press, NY & London.

There are numerous software products (both commercial and shareware)
available for Windows based software, as I believe the Leica Control Program
is that will password protect any volume. As for the hardware, your Laser Box
should have a key for the ignition. If you have any further questions, please
do not hesitate to contact me. I am sorry we did not have a Confocal
Microscope available at the time for your evaluation, however we have two new
products coming for your future consideration.
Good Luck,
Lawrence Kordon
Confocal Specialist
Nikon, Inc.




From: kaurin-at-rmslab.rockefeller.edu
Date: Wed, 20 Sep 1995 10:47:26 EDT
Subject: tem,immunogold, collagen and cell dyes

Contents Retrieved from Microscopy Listserver Archives
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This is a second posting of the original message as I have gotten many
messages of returned mail that could not reach me at my personal address
please respond to "microscopy..."I look forward to your comments.

I am interested in locating a dye that will stain collagen and or
a monolayer (huve cells) prior to embedding in LRW for post embd immuno-
labeling. This is simply to aid location of the monolayer while facing.
I have found a reference using 1% glut, 0.2% picric acid and 6mM eserine
(physostigmine). Does the eserine function as a dye? I intend on using 0.5%
glut, with paraformaldehyde. The monolayers will be enclosed in resin
then mounted for the z axis. Any ideas? Shelley Landon Kaurin






From: nee-at-lanl.gov (Norman Elliott)
Date: Wed, 20 Sep 1995 09:12:39 -0600
Subject: LM image analysis

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Dear List,

We are considering purchasing an image analysis package called "Micro-Tome"
from Vaytek, Inc. in Fairfield, IA. This package porports to do digital
confocal microscopy using any conventional white light microscope and
digital deconvolution of the acquired images. Does anyone use this product
and have an opinion regarding its usefulness or, more generally, does
anyone have an opinion regarding this approach to confocal microscopy
compared to the conventional laser based approach. All responses will be
kept confidential.




Norman Elliott | E-mail: nee-at-lanl.gov
Los Alamos National Lab | Fax: 505-665-2104
MST-7 MS E549 | Voice: 505-667-1587
Los Alamos, NM 87545 |






From: tvoiles-at-unlinfo.unl.edu (Todd Voiles)
Date: Wed, 20 Sep 1995 11:31:23 -0400
Subject: New WWW Sight for EM Facility...Includes a Multimedia SOP for an SEM

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Message-Id: {9509201632.AA13629-at-unlinfo.unl.edu}
X-Sender: tvoiles-at-129.93.1.11
X-Mailer: Windows Eudora Version 1.4.4
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

A new WWW site has just been put on-line

The Center for Materials Research and Analysis' Central Facility for
Electron Microscopy now has a site detailing:

What it is
What it can do
Where it is
How you can come to use it
Current events and research projects
Description of equipment with pictures
Sample output of Microscopes
Lots of documentation on equipment use and policies

Highlights include

A multimedia (mainly images) Standard Operating Procedure for our JEOL
JSM-840A SEM

An electronic scheduling form allowing users to set up times on microscopes
and submit them by e-mail

It can be accessed at the following

{http://www.unl.edu/tvoiles/}


Please visit the site and tell us what you think

Please DO NOT direct the replies to me (Todd Voiles
tvoiles-at-unlinfo.unl.edu) since I am leaving as of the 21st

PLEASE DIRECT your replies to the new webmaster Gary Krichau
(gkrichau-at-unlgrad1.unl.edu)

By the way, I'm leaving the list as well as my position here so Bye,
Bye...its been fun.




Todd Voiles
Central Facility Specialist
Central Facility for Electron Microscopy
Center for Materials Research and Analysis

University of Nebraska at Lincoln

tvoiles-at-unlinfo.unl.edu






From: images1-at-biosci.mbp.missouri.edu (Michael Stanley)
Date: Wed, 20 Sep 1995 17:25:08 -0500
Subject: cryo-stages for LM

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Message-Id: {v01510101ac8644f887fd-at-[128.206.15.185]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Hello All,

We have a user that is looking for a quality cryo-stage (freezing stage)
that will freeze down to -80 to -120 C if possible, and will fit onto a
Nikon Diaphot.

Has anyone collected a list of vendors lately? How about helpful hints, or
possible pitfalls to look out for, for both purchase and use.

TIA,

C. Michael Stanley, Ph.D.
Coordinator, Associate Director
Molecular Cytology Core Facility
Molecular Biology Program
2 Tucker Hall
University of Missouri-Columbia
Columbia, MO. 65211
(314) 882-4895
fax= 314-882-0123






From: ARGIL
Date: Wed, 20 Sep 1995 20:10:30 -0400 (EDT)
Subject: Microscopes, photography, aquaria

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To Steve Murrison -

If you Email your address to us, we will send you a catalog of imported
microscopes, as well as advice on video and photographic setups.
Our scopes are very high quality and inexpensive.
We have a nice little book called "The Microscope and How to Use It",
which is an excellent and practical introduction to the whole subject.

Arthur Gillman
Princeton, NJ





From: Silvio Flati :      FLATI-at-cmns.mnegri.it
Date: Thu, 21 Sep 1995 10:03:34 +0200 (MET-DST)
Subject: immunocytochemistry

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Hi to everybody!

I am a beginner in the techniques of immunocytochemistry
and I need some theoretical "background" concerning
the labelling with antibody and PAG staining.
Would you be so kind to advice me the title
of some interesting book?

Thanks in advance

Flati Silvio

Laboratory of Molecular Neurobiology
Mario Negri Sud Institute (Italy)




From: Paul Webster :      Paul.Webster-at-QuickMail.Yale.edu
Date: 21 Sep 1995 09:15:58 -0400
Subject: Immunocytochemistry

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Message-Id: {n1400447340.99545-at-QuickMail.Yale.edu}
"Silvio Flati" {FLATI-at-cmns.mnegri.it}
X-Mailer: Mail*Link SMTP-QM 3.0.2

For a good, comprehensive text on immunocytochemical methods try "Fine Structure
Immunocytochemistry" by G. Griffiths, 1993, Springer Verlag.

Another way to get quickly up to speed is to attend one of the annual methods
courses offered by the European Molecular Biology Organization. I can give you
details if you contact me directly.

Also, why not check out the new WWW site which has a methods book foccussing on
immunocytochemical methods. The URL is {http://info.med.yale.edu/cellimg} .

Paul Webster
Center for Cell Imaging
Yale School of Medicine
New Haven, CT 06520






From: wise-at-VAXA.CIS.UWOSH.EDU
Date: Thu, 21 Sep 1995 09:16:43 +0000
Subject: Ultramicrotomes

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We are putting together a request for $ for a new ultramicrotome to
replace our 25-year-old LKB. My favorite instrument of all time is the
Reichert Ultracut E and I have had no expeience with the Ultracut S or
later versions. Also I have not familiar with ultramicrotomes by other
manufacturers.
Does anyone out there have any insights, opinions, praise, etc for
any one particular instrument? In other words, given the money, which one
would you buy? Are used or rebuilt units available?
Please reply directly to me as all responses will be kept confidential.

Thanks in advance

Bob


Robert R. Wise, PhD
Director, UWO Electron Microscope Facility
Department of Biology
University of Wisconsin Oshkosh
Oshkosh, WI 54901
(414) 424-3404 tel
(414) 424-1101 fax
wise-at-vaxa.cis.uwosh.edu






From: ychen-at-macc.wisc.edu
Date: Thu, 21 Sep 1995 10:39:57 -0600
Subject: Re: TEM of polyester filter

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Message-Id: {v02120d01ac873bca95a1-at-[144.92.132.30]}

Hayes,

Last week I replied your massage to the microscopy net. Here I will
provide your more information of the usage of LVSEM to look at filter. You
can check the article of "LVSEM for high resolution topographic and density
contrast imaging", by Jim Pawley, in "Advances in electronics and eletron
physics", Vol. 83, pp203-274, (1992). This article discuss the priciple
and application of LVSEM in details. For your project, you can pay special
attension to the stereo pair of high resolution images of filter (Fig. 22
at p.250).

If you have any question or need help for using the LVSEM, please contact
me off line.

Ya Chen

=========================================================================
\ / Assistant Researcher-Cryo/SEM Coordinator TEL : 608-263-8481
\ / __ Integrated Microscopy Resource (IMR) FAX : 608-265-4076
\/ / / University of Wisconsin-Madison
/ / / 1675 Observatory Drive #167 Email:YChen-at-macc.wisc.edu
/ /__/_ Madison, WI 53706 Email:chen-at-calshp.cals.wisc.edu

IMR WWW Home Pages: http://www.bocklabs.wisc.edu/imr/imr.html
=========================================================================



} Hayes,
}
} The best way of imaging the surface of polyester filter is to look at them
} by high-resolution SEM (low-voltage FESEM). It can view surface features
} directly without complicated specimen preparation. I looked at some kind
} of filter 4 years ago in the Hitachi S-900 LVSEM. The filter surface can
} be imaged up to 100,000x in stereo. The 3-dimensional 5-10nm surface
} details are clearly seen at this magnification. Hope this information can
} help you.
}
} Ya Chen
}

} }
} } I have a customer who wants TEM surface characterization of polyester
} } filters which are "clean and dry". Pore sizes are 0.05 - 0.03 microns.
} } They are interested in the pore size, shape and distribution on both top
} } and bottom surfaces.
} } ......
} }
} } Fred A. Hayes






From: James Lausier :      yojimbo-at-itsa.ucsf.EDU
Date: Thu, 21 Sep 1995 11:33:00 -0700 (PDT)
Subject: Re: confocal microscopy

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On Tue, 19 Sep 1995, rutledge phil wrote:

}
} We have just purchased a Leica confocal system which will be used by the
} faculty and grad students here at UMBC. I would like to build a good
} library of reference books for their use and was wondering if anybody had
} good suggestions as to what books would be a necessity and what books would
} be nice to have. Also, does anybody know of a good security system for
} the microscope so non-trained personnel won't have access to using the
} scope. It would be nice if the system could be incorporated into the
} software of the confocal system. Any suggestions would be greatly
} appreciated.
}
} Thanks,
}
} Phil Rutledge, Director
} Center for Electron Microscopy
} University of Maryland Baltimore County
} 5401 Wilkens Ave.
} Catonsville, MD 21228
}
Phil I have just started reading The Image Processing Handbook, second ed.
by John C. Russ, CRC Press. It is a great text for digital imaging
concepts and techniques. I would think it would be of value in a con
focal library.

Good luck, James Lausier




From: jouneau-at-cime.epfl.ch (Pierre-Henri Jouneau)
Date: Thu, 21 Sep 1995 22:10:38 +0200
Subject: Announcement: Electron Microscopy Yellow Pages

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X-Sender: phjounea-at-cimedec1.epfl.ch
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This is to let you know you about the
Electron Microscopy Yellow Pages

http://cimewww.epfl.ch/emyp/

These EM Yellow Pages are intended to provide extensive
and comprehensive links to electron microscopy resources
inside the World Wide Web: laboratories, societies, educational
resources, conferences, news and publications, software,
equipment & consulting, data & databases, ...

If you know of useful Web resources which are not to be found
in these pages, let me know, and I will update the list.

I am also looking for ways to improve these Yellow Pages.
So if you have comments or suggestions, please email me.


Sincerely yours,

Pierre-Henri Jouneau
Centre Interdepartemental de Microscopie Electronique
Ecole Polytechnique Federale de Lausanne, Switzerland
http://cimewww.epfl.ch






From: richard.easingwood-at-stonebow.otago.ac.nz (Richard Easingwood)
Date: Fri, 22 Sep 1995 09:16:40 +1100
Subject: Cytochemistry on Lowicryl sections

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We are trying enzyme cytochemistry on spinal cord however all our attempts
to slice the lightly fixed cord on vibratomes, tissue choppers etc result
in spinal cord "soup". Cutting the thin slices of cord seems almost
impossible.
One option we thought of exploring is to lightly fix fix the cord,
cryoprotect then freeze, cryosubstitute into Lowicryl and then attempt
enzyme cyotchemistry on the Lowicryl semithin sections.
Has anyone tried enzyme cytochemistry on Lowicryl or on other acrylic sections?

Allan Mitchell

Please reply to: richard.easingwood-at-stonebow.otago.ac.nz

Richard Easingwood
South Campus Electron Microscope Unit
Otago Medical School
PO Box 913
Dunedin
NEW ZEALAND

Telephone: 64-03-479 7301
Facsimile: 64-03-479 7254

The southernmost electron microscope unit in the world






From: INGRAM-at-RTI.ORG
Date: Thu, 21 Sep 1995 18:07:58 -0400 (EDT)
Subject: North Carolina Society for Microscopy and Microbeam Analysis Meeting

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Here is the Program for the NCSMMA Annual Meeting Sept 29 - Oct 1 at lovely
Wrightsville Beach NC

For more info on Registration etc, please call Ms. Betty Gooch, Symposium
Coordinator at: (919) 684-3534

DUKE UNIVERSITY MEDICAL CENTER
and the
NORTH CAROLINA SOCIETY FOR MICROSCOPY
AND MICROBEAM ANALYSIS

present the

FOURTEENTH annual
SYMPOSIUM ON
ADVANCES IN MICROSCOPY

RMicroscopy Outreach: Conveying Its Science, Art and TechnologyS
Holiday Inn, Wrightsville Beach, North Carolina
September 29-October 1, 1995

PROGRAM
Friday, 29 September 1995
4:00 - 6:00 pm REGISTRATION and refreshments - Holiday Inn at Wrightsville Beach
6:30 - 8:30 pm Barbeque - poolside at the Holiday Inn, Wrightsville Beach
Courtesy of JEOL (USA) Inc.
Beverages courtesy of AMRAY Inc.
(Complimentary with Registration; Adult and Children Guests $10/$5)

Saturday, 30 September 1995
8:30 - 11:30 am Special Workshops/Tutorials on:
Specimen Preparation for Materials Sciences - Ron Anderson
Cryotechniques - Stacie Kirsch
8:00 - 10:00 am Coffee, juice and donuts - Holiday Inn
10:00 - 11:30 am Poster Session, ExhibitorsU Displays - Holiday Inn
11:30 - 12:00 Noon NCSMMA Annual Business Meeting
12:00 - l:00 pm Lunch - Casual buffet

1:00 - 1:05 pm P Welcome P
1:05 - 1:35 pm "Science and Public Policy in North Carolina Today"
Quentin Lindsey
1:45 - 2:15 pm "Hands on Science Using Microscopy: the MSA MICRO Project"
Michael Isaacson
2:30 - 2:45 pm P Coffee Break P
2:45 - 3:15 pm "A Delivery System for Project MICRO"
Dick Ward
3:30 - 4:00 pm "Working Programs: Successes and Pitfalls"
Karen Shafer
4:15 - 4:30 pm COFFEE BREAK - Informal discussion
4:30 - 5:00 pm "Navigating the Internet and Digital Imaging"
Nestor Zaluzec
5:00 - 6:15 pm Workshop: TelePresence Microscopy and the Internet
Nestor Zaluzec
5:00 - 6:30 pm Poster Session, ExhibitorsU Displays
7:00 pm Evening Buffet - Al Fresco at the Holiday Inn, Wrightsville Beach
Supported in part by Oxford Instruments and Zeiss (Electron Optics Division)

Sunday, 1 October 1995
7:30 - 8:30 am Coffee, juice, and donuts - Meeting Room, Holiday Inn
8:30 - 9:00 am Student Awards and Presentations
9:00 - 9:30 am "An Early Visit to Inner Space--a Personal Voyage"
John Watson
9:45 - 10:15 am "Imaging Muscle: The Technology, the Art, the Science"
Ann Goldstein
10:30 - 11:00 am COFFEE BREAK
11:00 - 11:30 am "A Computer Network Laboratory for Microscopy Education"
Gary Chandler
11:45 - 12:15 pm "Washington Outlook: Is There a Contract Out on Science?"
Pat Calarco
1:00 - 2:-00 pm Lunch



** SORRY ABOUT THE ODD LETTERS APPEARING ..... THE TEXT EDITOR DOESN'T LIKE
ITALICS!!
MAIL
SEND






From: RLMCGILL-at-eastman.com (rick mcgill)
Date: Fri, 22 Sep 1995 07:28:50 -0400
Subject: subscribe

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Message-Id: {199509220609.IAA12973-at-gate.sbbio.be}

I wish to subscribe to the microscopy list server. Thanks!!

RLmcgill-at-eastman.com
Rick McGill
Eastman Chemical Company
P.O. Box 1972
Kingsport, TN 37662-5150
423-229-5473
E:mail RLMCGILL-at-EASTMAN.COM





From: RLMCGILL-at-eastman.com (rick mcgill)
Date: Fri, 22 Sep 1995 08:03:12 -0400
Subject: subscribe

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I wish to SUBSCRIBE


Rick McGill
Eastman Chemical Company
P.O. Box 1972
Kingsport, TN 37662-5150
423-229-5473
E:mail RLMCGILL-at-EASTMAN.COM





From: CHAFFEYN :      NIGEL.CHAFFEY-at-bbsrc.ac.uk
Date: Fri, 22 Sep 1995 15:28:07 +0000
Subject: Sticky section - a solution

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Dear Fellow Microscopists,
Many thanks to all who replied to me recent plea for help regarding
sticking methacrylate sections to glass slides. I received lots of advice
which I am still in the process of evaluating. However, the best 'solution' so
far appears to be Mayere's egg albumin - which is consistently giving a
section-retention rate greater than 90%. I think it just goes to prove that
there is nothing new in microscopy...
Best wishes to everyone!
Nigel Chaffey: IACR-Long Ashton Research Station, Dept Agric. Sci.,
Univ. Bristol, Long Ashton, Bristol BS18 9AF, UK (email -
nigel.chaffey-at-bbsrc.ac.uk)




From: Jay Jerome :      jjerome-at-isnet.is.wfu.edu
Date: Fri, 22 Sep 1995 11:55:10 -0400 (EDT)
Subject: Re: Cytochemistry on Lowicryl sections

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On Fri, 22 Sep 1995, Richard Easingwood wrote:

}
} We are trying enzyme cytochemistry on spinal cord however all our attempts
} to slice the lightly fixed cord on vibratomes, tissue choppers etc result
} in spinal cord "soup". Cutting the thin slices of cord seems almost
} impossible.
} One option we thought of exploring is to lightly fix fix the cord,
} cryoprotect then freeze, cryosubstitute into Lowicryl and then attempt
} enzyme cyotchemistry on the Lowicryl semithin sections.
} Has anyone tried enzyme cytochemistry on Lowicryl or on other acrylic sections?
}
} Allan Mitchell

As an alternative, try cutting cryosections and running the cytochemistry
on the sections, then embedding. Alternatively, put the whole slice on a
grid and cover with a drop of spurr resin. Cure the resin and view the
"thick" section at the highest accelerating voltage your microscope
allows. We have viewed up to 1 um at 120 KeV with our Philips 400 and up
to 5 um with our CM-300 at 300 KeV.


Best

Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-isnet.is.wfu.edu *
**************************************************************





From: f.m.haug-at-basalmed.uio.no (Finn-Mogens Haug)
Date: Fri, 22 Sep 1995 19:04:37 +0200
Subject: Standards for fluorescence microscopy

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Message-Id: {199509221704.TAA14535-at-pons.uio.no}
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Dear list,

We have looked mostly in vain for fluorescent standards, specifically
"non-bleaching", solid, inorganic, specimens suitable for routine checks
on excitation level and checks on spatial uniformity of the optical/digital
imaging system.

Reports and handbooks describe the use of uranyl-glass and inorganic
crystals, but the only commercial product we have found contains a 1-2 mm
circular fluorescing area of unknown composition, mounted on a microscope
slide. Price: USD 1400 incl VAT. Expensive?

A local contact makes Yttrium-Oxy-Sulfide crystals with discrete
emission/excitation
bands for use in microspectrofluorimetry. However, spectral specificity may
not be required for checks on excitation level and the powder's granularity
may preclude its use in testing pixel-to-pixel spatial uniformity.

Names, fax/phone-numbers and e-mail adresses of suppliers of such standards
would be most helpful, perhaps to others as well.


Thanks in advance -


Finn-Mogens Haug

Department of anatomy E-mail: f.m.haug-at-basalmed.uio.no
Institute of basic medical sciences Phone : +47 22 85 12 67
University of Oslo Fax : +47 22 85 12 78





From: William Tivol :      tivol-at-wadsworth.org
Date: Fri, 22 Sep 1995 13:28:08 -0400 (EDT)
Subject: Re: tem

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}
} Fellow microscopists,
} I need to digitise some tem negatives with a view to carrying out 3D
} reconstruction. If anyone is currently using a digitising device
} capable of high resolution I would like to hear about it.

Dear Chris,
We are using a Perkin-Elmer microdensitometer and/or a CCD array for
these kinds of measurements.

} minimum spot size

5 micron square aperture on the P-E, ~40 micron on CCD.

} speed of scanning

Up to 40 mm/sec on P-E, but slower speeds are useful for diffraction,
where there is lots of ~0 OD with a few intense spots. In this case, the
slower speed gives better quantitation. The CCD can scan a micrograph in a
few seconds.

} optical density range

0-4 OD on the P-E, 0-~2 on the CCD

} grey levels

~1000 on the P-E (more may be available on a newer instrument), fewer
on the CCD, but I don't know how many.

} cost

A reconditioned P-E may be available for ~20,000, a CCD-type scanner
can be put together for less.
If you want to try either of our systems or consult with any of the
group who does 3D reconstruction for a living, write to Dr. Joachim Frank,
Wadsworth Center, Empire State Plaza, PO Box 509, Albany NY 12201-0509. He
or one of the crew can take it from there. Good luck.
Yours,
Bill Tivol




From: Richard F. Kuklinski :      rfk2-at-Ra.MsState.Edu
Date: Fri, 22 Sep 1995 16:09:40 -0500
Subject: SEM sample prep of ice cream

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I have been requested by an e-mail-deprived colleague to pass on
the following:

From Bill Monroe, Electron Microscope Center,
Mississippi State University

We have a request to process ice cream samples in order to
visualize the spores of Bacillus licheniformis which are dispersed
in the samples. The first fixation protocol (glutaraldehyde &
osmium tetroxide, centrifuging at all steps) resulted in only
being able to see the processed milk fat and no visible spores.
Can anyone suggest a method of removing the fat and leaving the
spores intact during processing?




From: Michael Rock :      merock-at-u.washington.edu
Date: Fri, 22 Sep 1995 14:51:43 -0700 (PDT)
Subject: Re: SEM sample prep of ice cream

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Bill-
I remember seeing a poster at Scanning '95 in the Oxford Instruments
booth, they were advertising their system and had some FDA researchers
images/spectra of ice-cream, sorry I don't have any further details.
Contact Pat Campos (415) 578-0202 for more info.
-Mike

On Fri, 22 Sep 1995, Richard F. Kuklinski wrote:

} I have been requested by an e-mail-deprived colleague to pass on
} the following:
}
} From Bill Monroe, Electron Microscope Center,
} Mississippi State University
}
} We have a request to process ice cream samples in order to
} visualize the spores of Bacillus licheniformis which are dispersed
} in the samples. The first fixation protocol (glutaraldehyde &
} osmium tetroxide, centrifuging at all steps) resulted in only
} being able to see the processed milk fat and no visible spores.
} Can anyone suggest a method of removing the fat and leaving the
} spores intact during processing?
}




From: Calvin Montgomery :      cal-at-marlin.ssnet.com
Date: Fri, 22 Sep 1995 19:40:09 -0400 (EDT)
Subject: subscribe

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Subscribe


cal-at-ssnet.com




From: Daniel Luchtel :      dluchtel-at-u.washington.edu
Date: Fri, 22 Sep 1995 17:59:38 -0700 (PDT)
Subject: Re: Sticky section - a solution

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X-Sender: dluchtel-at-homer24.u.washington.edu

Nothing new in microscopy????? How about confocal, various scanning
probe microscopies, acoustic, x-ray - a very short list of the
microscopies developed or greatly advanced in the last decade or so. I
think that there is a lot new in microscopy these days.

On Fri, 22 Sep 1995, CHAFFEYN wrote:

} Dear Fellow Microscopists,
} Many thanks to all who replied to me recent plea for help regarding
} sticking methacrylate sections to glass slides. I received lots of advice
} which I am still in the process of evaluating. However, the best 'solution' so
} far appears to be Mayere's egg albumin - which is consistently giving a
} section-retention rate greater than 90%. I think it just goes to prove that
} there is nothing new in microscopy...
} Best wishes to everyone!
} Nigel Chaffey: IACR-Long Ashton Research Station, Dept Agric. Sci.,
} Univ. Bristol, Long Ashton, Bristol BS18 9AF, UK (email -
} nigel.chaffey-at-bbsrc.ac.uk)
}




From: Carlos E. Barbosa :      grial-at-relay.starnet.net.ar
Date: 09/23/95
Subject: LM - petrology

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From: huffe-at-pgl7.chem.nyu.edu (Edward J. Huff)
Date: Mon, 25 Sep 1995 10:09:30 -0400
Subject: Re: Standards for fluorescence microscopy

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I hope I haven't been wasting my time on something that is already
solved, but the previous message suggests that this is not easily
available.

I have been working on measuring the illumination for quantitative
fluorescence microscopy. I obtained a slab of uranyl glass some time
ago, but it was too thick to be useful. (I got it from a supplier
that I found from a message on the confocal mailing list.).

I read a little about toxcicity of Uranium and decided that normal
care is adequate in dealing with the stuff. Uranyl glass is apparently
about 1% Uranimum, and maximum inhalation is around 1 mg per day.
The chemical poison effects are more important than the radioactivity.

I broke a piece of it up and ground it in a porcelain morter to produce
a fine white powder. I managed to get a 10 micron thick layer of
closely packed pieces mounted in acrylic media, and I programmed
the XY table to move this sample in random rotary motion. This
give a fairly good measurement of the illumination, and apparently
the noise in this measurement is comparable to the noise from other
sources.

This sample (which I got this thin by putting it in a 1 ton press
while the toluene was evaporating) has been degrading: the acrylic
is flowing and voids are forming. I just got some acrylic monomer
and if necessary I will make a new sample of ground up glass mounted
in acrylic which should be dimensionally stable after curing.
However, I plan to make a uniform thin sample by melting it.

I obtained a "test kiln" for $150 from a local ceramics supply store.
It has a thermocouple and goes up to 2000F (1100C). I found that a
small sample of Uranyl melts and forms a sphere below 1100C. Unfortunately
the slides I have been using melt around 100C lower than the Uranyl glass.

I have ordered a quartz slide and coverslip and plan to prepare a sample
a micron or two thick by placing a small amount (say 5mg) of powder on
the slide and melting it with a coverslip.

The CRC handbook says quartz strain point is 1070C, anealing point
approx 1140C, softening point approx 1665C, so there should be no
difficulty melting the uranyl glass without affecting the quartz.
I plan to let it cool slowly.

Uranyl glass came from:
Newport Industrial Glass, Inc.
2044-C Placentia Ave
Costa Mesa, California USA 92627
Contact Bill Larson
Phone (714)642-9980
Fax (714)642-4832

Quartz slide is on order from Ted Pella, Inc.
tedpel-at-snowcrest.net, tedpel-at-aol.com
POBox 492477
Redding, CA 96049-2477
800-237-3526 (USA) 800-637-3526(Calif) 800-243-7765 (Canada)




From: Skip Potter :      potter-at-vitek.com
Date: Mon, 25 Sep 1995 09:51:28 -0500 (CDT)
Subject: Assistance-Bacterial Micrographs

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Hello-

I am with a biotech company in St. Louis. Our products are automated
microbiology and immunology systems for larger hospitals and labs. We
provide reporting of the identity and susceptibility to antimicrobials of
bacteria from patient samples and also the identity of viruses for
certain infectious diseases. We are an international company.

We have an ad hoc team working on a home page for the WWW. Rather then
using it for advertising purposes we want to have it as a resource center
for microbiologists and immunologists with links to other information
sites as well as monthly educational articles. For example we might have
articles on organisms that gained some attention in the media such as E.
coli 0157 or the ebola virus.

We are trying to locate an existing source for micrographs of both common
and unusual bacteria and viruses. We would use small images of these on
our home page. We would prefer to have them in a format where we could
have them colorized. The idea is more of one to capture people's
attention rather then expecting that the casual reader would use them for
scientific purposes.

If anyone can provide assistance we could acknowledge their contribution
on our home page and cover nominal reproduction and mailing expenses.

Thank you in advance-




From: STEVEN E SLAP :      75767.640-at-compuserve.com
Date: 25 Sep 95 11:50:04 EDT
Subject: Re: SEM of ice cream

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Richard-
Michael Rock's post to you concerned an Oxford cryopreparation system. Such a
system allows you to directly view frozen hydrated specimens. There are three
manufacturers of thses systems, Oxford being one. Energy Beam Sciences is the
exclusive U.S. distributor for VG Microtech (Polaron) who make another. Please
fax me directly for details.
Steven Slap





From: Skip Potter :      potter-at-vitek.com
Date: Mon, 25 Sep 1995 09:51:28 -0500 (CDT)
Subject: Assistance-Bacterial Micrographs

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---------- Forwarded message ----------

Hello-

I am with a biotech company in St. Louis. Our products are automated
microbiology and immunology systems for larger hospitals and labs. We
provide reporting of the identity and susceptibility to antimicrobials of
bacteria from patient samples and also the identity of viruses for
certain infectious diseases. We are an international company.

We have an ad hoc team working on a home page for the WWW. Rather then
using it for advertising purposes we want to have it as a resource center
for microbiologists and immunologists with links to other information
sites as well as monthly educational articles. For example we might have
articles on organisms that gained some attention in the media such as E.
coli 0157 or the ebola virus.

We are trying to locate an existing source for micrographs of both common
and unusual bacteria and viruses. We would use small images of these on
our home page. We would prefer to have them in a format where we could
have them colorized. The idea is more of one to capture people's
attention rather then expecting that the casual reader would use them for
scientific purposes.

If anyone can provide assistance we could acknowledge their contribution
on our home page and cover nominal reproduction and mailing expenses.

Thank you in advance-





From: Robert Fisher :      rmfisher-at-u.washington.edu
Date: Mon, 25 Sep 1995 17:13:31 -0700 (PDT)
Subject: HELP - Staining Polymers and Wax

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Message-Id: {9509251645.AA61164-at-acs1.acs.ucalgary.ca}


Prof. Ed Haskins (UW Botany) and I are trying to
find some optical microscopy stains that will distinguish
between microscopic bits and blobs of adhesives (Scotch tape
-TM noted), other adhesives, paraffin (and hopefully other
polymers) and cellulose.

Sudam IV shows a glimmer of promise but the red-brown color
is close to the cellulose fibers.

All suggestions gratefully acknowledged.

Use your own judgement on open or closed replies.

Bob Fisher
Fax 206 543-3100







From: Gunnel.Karlsson-at-vf.slu.se (Gunnel Karlsson)
Date: Tue, 26 Sep 1995 16:25:38 +0100
Subject: Diamond knives

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Message-Id: {199509261525.QAA10549-at-alnus.slu.se}
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Hello everyone
I'm a biologist (botany) and have been using diamond knives (45=B0) for both
semithin and ultrathin sections for many, many years. I have done some
sectioning of embedded zeolite crystals, with the 45=B0 biological knife,
with some success.
I've noticed that people in material science are more and more interested
in sectioning and some companies have diamond knives for inorganic
specimens, either 35=B0, 45=B0, or 55=B0, and that they are less expensive t=
han
diamond knives for biological applications.
Does somebody have some experience of these diamond knives for material
science? What angle do you recommend? We are going to buy a new set of
diamond knives for a new centre for EM and I should very much appreciate
some (a lot) advice about the material science part. The samples will vary
from polymers over biological specimens to organic and inorganic crystals,
i e it will cover the whole range of hardness.

Thanks in advance
Gunnel Karlsson






From: wise-at-VAXA.CIS.UWOSH.EDU
Date: Tue, 26 Sep 1995 11:18:25 +0000
Subject: Technique

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Somebody recently posted a note briefly describing a technique that
involved embedding whole cells in Spurrs epoxy then viewing in the TEM. A
drop of epoxy was placed on cells on a cover slip, cured, then viewed
unsectioned at high kV (120-300 kV). Of course, I erased the original
message. Could the author please send me the protocol again?

Thanks

Bob


Robert R. Wise, PhD
Director, UWO Electron Microscope Facility
Department of Biology
University of Wisconsin Oshkosh
Oshkosh, WI 54901
(414) 424-3404 tel
(414) 424-1101 fax
wise-at-vaxa.cis.uwosh.edu






From: DVCCO-at-aol.com
Date: Tue, 26 Sep 1995 12:12:04 -0400
Subject: Web Site Camera / Grabber Guide

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Monochrome real time digital RS-422 (10 or 8 bits) and analog RS-170 10 bit
CCD video cameras are presented in a informative web site, containing a
question and answer section on how to go about choosing the proper camera for
the application, what to look for in a camera, system configuration issues,
and a complete RS-422 frame grabber selection guide listed by bus. Complete
plug and play systems can be provided for PCI,Nubus,S-bus & SGI digital.
--------------------------------------------------------------------------
(((((((( The URL is: http://www.edt.com/dvc/dvc.html ))))))))))
--------------------------------------------------------------------------




From: Doug Davis :      doug_davis-at-maillink.berkeley.edu
Date: 26 Sep 1995 09:45:44 -0700
Subject: Sale- Bio prep equipment

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Message-ID: {n1400014149.20385-at-maillink.berkeley.edu}

Subject: Time: 9:28 AM
OFFICE MEMO Sale: Bio prep equipment Date: 9/26/95

FOR SALE:

Life Cell CF 100 with Edwards mechanical pump, SS backing line, LN trap,
LN supply line w/solenoid valve, nitrogen gas regulator and all
accessories. Vintage1989, used perhaps 3 or 4 times.

Denton 502A, w manual valving and oil diffusion pump, two pairs of electrodes,
LN trap w/funnel. Vintage 1988- THIS UNIT HAS NEVER BEEN USED!!! Sparkling
clean. Pumps have been run only.

Contact: Doug Davis, EML Berkeley
(510) 642-2085
(510) 643-6207 fax
doug_davis-at-maillink.berkeley.edu





From: William Tivol :      tivol-at-wadsworth.org
Date: Tue, 26 Sep 1995 12:52:13 -0400 (EDT)
Subject: Re: SEM sample prep of ice cream

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} } From Bill Monroe, Electron Microscope Center,
} Mississippi State University
}
} We have a request to process ice cream samples in order to
} visualize the spores of Bacillus licheniformis which are dispersed
} in the samples. The first fixation protocol (glutaraldehyde &
} osmium tetroxide, centrifuging at all steps) resulted in only
} being able to see the processed milk fat and no visible spores.
} Can anyone suggest a method of removing the fat and leaving the
} spores intact during processing?
}
Dear Bill,
Do you need to see the spores *in* the ice cream? If not, you could
dissolve away or dilute the water-soluble part, then separate the spores
either by adding organic solvent and filtering or sedimenting. I'd suggest
forgetting the OsO4 until the lipid component has been removed; the OsO4 only
makes the lipid darker and harder to solubilize. Spores should be hardy
enough to survive some pretty harsh isolation steps.
To examine the spores in situ, I'd cut frozen sections, warm to ~-40
try to infiltrate UAc (does it dissolve in an ethanol-water mixture?), recool
to LN2 and see if I could see anything. Higher voltage might be a big help--
Non-disclaimer: I have a vested interest in attracting users to our 1.2 MV
microscope. Good luck.
Yours,
Bill Tivol




From: Miguel Avalos B. :      miguel-at-ifuname.ifisicaen.unam.mx
Date: Tue, 26 Sep 1995 10:09:59 -0700
Subject: DENTON..

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Message-ID: {n1400008738.41971-at-maillink.berkeley.edu}

Reply to: RE} DENTON..

Miguel- Carbon evaporation is one of the primary uses this instrument is
designed for and it is a very reliable unit with good tech support from
Denton. This evaporator has a value of about $13,000 in today's dollars. We
are accepting offers but donations won't fly with managment -especially new,
unused equipment. -Doug

--------------------------------------
(1.38.193.5/16.2) id AA14219; Tue, 26 Sep 1995 10:09:59 -0700

Is your Denton 502A system suitable for carbon evaporation ?? and
how much are you asking for it ???

Yours

Miguel Avalos
Ensenada, B.C., MEXICO

P.d. Would you consider donations ???






From: Doug Davis :      doug_davis-at-maillink.berkeley.edu
Date: 26 Sep 1995 11:26:09 -0700
Subject: Re: TEM of a polymer

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Message-ID: {n1400008158.82400-at-maillink.berkeley.edu}

Subject: Time: 11:22 AM
OFFICE MEMO RE} TEM of a polymer Date: 9/26/95

Huyen- Perhaps chlorosulfonic acid; it works on polyethylene. You might try
the book "Polymer Microscopy" by LindaC. Sawyer snd David T. Grubb, ed.
Chapman and Hall, 1987. -Doug





From: Miguel Avalos B. :      miguel-at-ifuname.ifisicaen.unam.mx
Date: Tue, 26 Sep 1995 12:57:49 -0700
Subject: Re: DENTON..

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From: PRC-at-bragg.bio.purdue.edu
Date: Tue, 26 Sep 1995 16:36:04 -0500 (EST)
Subject: Kodak SO-163 speed?

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Does anyone know the film speed of Kodak SO-163 developed in full
strength D-19 for 12 minutes using an accelerating voltage of 200 kv. So far
I have been unable to find this information. Thus far, my efforts include
speaking with the Tech support people at Kodak, searching the Kodak web page
and reading the data provided with the film itself. Any suggestions or
answers would be greatly appreciated.

THANKS,

-----------------------------------------------
| Paul Chipman |
| Department of Biological Science |
| Purdue University |
| West Lafayette, IN 47907 |
| email: prc-at-bragg.bio.purdue.edu |
| Phone: (317)494-5643 |
| FAX: (317)496-1189 |
-----------------------------------------------







From: Jay Jerome :      jjerome-at-isnet.is.wfu.edu
Date: Tue, 26 Sep 1995 18:28:03 -0400 (EDT)
Subject: Re: Technique

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To reiterate and expand on our embedding of thick cryosections:

The method we use is a modification of the Tokuyasu procedure. Lightly
fixed, cryoprotected, Cryosections 1-4 um are cut and mounted onto a
formvar-coated, carbon stabilized grid. The section is thawed, washed in
Gey's balanced salt (this seems to preseve antigenicity of proteins
better than standard buffers) and then immunostained using whatever
procedures are desired. We transfer the grids to drops of solution
containing the various washes and stains. After immunostaining we fix
again in Glutaraldehyde, wash, osmicate, dehydrate, and then embed by
blotting off excess 100% alcohol, and placing a drop of LR White or Spurr
resin on the section. We blot the resin coated section between two pieces
of filter paper, then place the grid on clean filter paper and heat cure
the resin. Once cured the sections can be stained with lead citrate or
uranyl acetate.




Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-isnet.is.wfu.edu *
**************************************************************





From: hris-at-facstaff.wisc.edu (Hans Ris)
Date: Tue, 26 Sep 1995 22:52:59 -0500
Subject: silane sticky substrates

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Message-Id: {m0sxkEb-0000UfC-at-deep.rsoft.bc.ca}

We noticed recently in this place interest in preparing sticky
substrates through treatment with silane. We would like to tell you about a
method that we have now used routinely for about 5 years. It is based on
procedures published by JP Robinson, P.Dunnill, and MD Lilly, in
Biochim.Biophys.Acta 242, 659-661, 1971, and M.Buechi and T.Baechi, in
J.Cell Biol. 83, 338-347, 1979.
1. The glass (or other carriers)must be absolutely clean. This can be
accomplished by immersing for several hours in aqueous 20% sulfuric acid, or
20% hydrochloric acid in ethanol.I prefer washing with Bon Ami cleaning
powder until the surface is hydrophylic.
2. The clean carriers are soaked for 2 h in waterfree acetone that had been
stored for at least 24 h over molecular sieve).
3. Prepare a 2% solution of 3-aminopropyltriethoxysilane (Aldrich Chemical
Co Milwaukee WI) in waterfree acetone. The carriers are placed in
appropriate containers (glass,nalgene, polypropylene) which must be totally
filled with the silane solution , and stored for 24 h at 50 degree C. If you
use a 4% silution of silane, the carriers will be ready for use in 12 h. The
silane treatment coats the carriers with amino groups.
4. To attach fresh cells or cell fractions to the carriers, they are rinsed
in acetone and immersed for one hour in 1% glutaraldehyde. The carriers are
then placed into dist. water at 4 degree C. They can be kept for about 5
days. Their surface is now coated with aldehyde groups, which bond
covalently with aminogroups on the surface of cells or cell fractions. If
cells or cell fractions must be fixed before attachment( in glutaraldehyde
fixative) the 1% glutaraldehyde is omitted. Exposed aldehyde groups on the
fixed biological materials will bond to the amino groups on the carriers.
We have used sticky carriers prepared by this procedure to attach
cells previously fixed in suspension to glass carriers for imaging by field
emission SEM ( Malecki and Ris 1990, Scanning 13 ,82 ; Malecki and Ris 1992,
Scanning 14, 76; Malecki,1991, Scanning Microscopy,Suppl.5, S 53). Silane
treated coverslips were used to attach isolated amphibian oocyte nuclear
envelopes for imaging of nuclear pore complexes by FESEM ( Ris, EMSA
Bull.21, 54, 1991. ) Such coverslips were also used to retain sections of
epon embedded biological materials, after extraction of the epon for FESEM
imaging ( Ris and Malecki, 1993. J.Struct.Biol. 111,148).
H.Ris and M.Malecki, Integrated Microscopy Resource, Univ. of Wisconsin,
Madison WI. hris-at-facstaff.wisc.edu - Malecki-at-macc.wisc.edu





From: f.m.haug-at-basalmed.uio.no (Finn-Mogens Haug)
Date: Wed, 27 Sep 1995 17:06:55 +0200
Subject: Re: Standards for fluorescence microscopy

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Message-Id: {m0sxoD7-0000ehC-at-deep.rsoft.bc.ca}

There were several responses to my query before the week-end,
on "permanent standards for fluorescence microscopy".

The question was posted to three lists; comments were received
there and directly. From the answers, fluorescing plastic blocks
or sections, chambers containing fluorescent fluid, and uranyl
glass, are practical standards for which some sources were given.
Most of this may be common knowledge, so I will only send
summaries directly on request.

One respondent asked if there were reasons for wanting to use
an inorganic standard, except ease of storage. I had thought
that inorganic standards, as uranyl glass, would be more stable
under excitation, particularly when testing lamp stability over
longer time. Apart from this, there is perhaps no advantage in
re-usng a standard **object** rather than **formulation**. (?)

Thanks for all the advice, Finn-Mogens.


Finn-Mogens Haug

Department of anatomy E-mail: f.m.haug-at-basalmed.uio.no
Institute of basic medical sciences Phone : +47 22 85 12 67
University of Oslo Fax : +47 22 85 12 78





From: William Tivol :      tivol-at-wadsworth.org
Date: Wed, 27 Sep 1995 12:47:51 -0400 (EDT)
Subject: Re: Kodak SO-163 speed?

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Paul Chipman wrote:
}
} Does anyone know the film speed of Kodak SO-163 developed in full
} strength D-19 for 12 minutes using an accelerating voltage of 200 kv. So far
} I have been unable to find this information. Thus far, my efforts include
} speaking with the Tech support people at Kodak, searching the Kodak web page
} and reading the data provided with the film itself. Any suggestions or
} answers would be greatly appreciated.
}
Dear Paul,
Our experience with SO163 indicates that one gains a factor of 3 to 4
going from the usual developing conditions (1:2 D-19 for 4 min) to push con-
ditions (D-19 full str. for 12 min). Although the film speed will vary with
accelerating voltage, the ratio should not, so if you can find out the film
speed under usual developing conditions for 200 kV electrons, multiply by
about 3.5 to get what you want. Good luck.
Yours,
Bill Tivol




From: wise-at-VAXA.CIS.UWOSH.EDU
Date: Wed, 27 Sep 1995 16:47:10 +0000
Subject: Re: Kodak SO-163 speed?

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Does any one out there have a source for Polaroid portable film processing
tanks? They are white, plastic, round, with a tightly fitting lid and have
a rack on the inside that holds about twelve 4"x5" negatives.

Bob


Robert R. Wise, PhD
Director, UWO Electron Microscope Facility
Department of Biology
University of Wisconsin Oshkosh
Oshkosh, WI 54901
(414) 424-3404 tel
(414) 424-1101 fax
wise-at-vaxa.cis.uwosh.edu






From: f.m.haug-at-basalmed.uio.no (Finn-Mogens Haug)
Date: Thu, 28 Sep 1995 13:20:21 +0200
Subject: Administrivia, please help

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Message-Id: {199509281119.MAA29334-at-pons.uio.no}
X-Sender: finnmog-at-pons.uio.no
X-Mailer: Windows Eudora Version 1.4.3
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

When registering to this list recently, I probably made some mistake.
The problem is that after submitting a message to the list, I receive
the error messages that arise when members of list are unreachable.
Thus, after submitting two messages I received altogether 35 error
messages. An example is enclosed at the end here.

A few days ago, I reported this to Postmaster-at-aaem.amc.anl.gov,
hoping that she would be a human being, able to point out what is
wrong, which she is probably not since there has been no reply
in point.

Perhaps one of the list members knows what is wrong - or whom to
contact about the problem.

Thanks in advance, Finn-Mogens.

******************************************************************

} Bad address -- {RLORNB-at-ccmail.monsanto.com}
} Error -- Message too old: %MULTINET-F-EHOSTUNREACH, No route to host
} Bad address -- {fstewartdavis-at-ppg.com}
} Error -- Message too old: Error sending MAIL command to ppg.com
} Bad address -- {vit-at-scvnet.com}
} Error -- Message too old: %MULTINET-F-EHOSTUNREACH, No route to host
}
} Start of returned message
}
} Message-Id: {199509221704.TAA14535-at-pons.uio.no}
} X-Sender: finnmog-at-pons.uio.no
} X-Mailer: Windows Eudora Version 1.4.3
} Mime-Version: 1.0
} Content-Type: text/plain; charset="us-ascii"
} Date: Fri, 22 Sep 1995 19:04:37 +0200
} To: microscopy-at-AAEM.AMC.ANL.GOV
} From: f.m.haug-at-basalmed.uio.no (Finn-Mogens Haug)
} Subject: Standards for fluorescence microscopy
}
} Dear list,
}
} We have looked mostly in vain for fluorescent standards, specifically
} "non-bleaching", solid, inorganic, specimens suitable for routine checks
} on excitation level and checks on spatial uniformity of the optical/digital
} imaging system.
}
} Reports and handbooks describe the use of uranyl-glass and inorganic
} crystals, but the only commercial product we have found contains a 1-2 mm
} circular fluorescing area of unknown composition, mounted on a microscope
} slide. Price: USD 1400 incl VAT. Expensive?
}
} A local contact makes Yttrium-Oxy-Sulfide crystals with discrete
} emission/excitation
} bands for use in microspectrofluorimetry. However, spectral specificity may
} not be required for checks on excitation level and the powder's granularity
} may preclude its use in testing pixel-to-pixel spatial uniformity.
}
} Names, fax/phone-numbers and e-mail adresses of suppliers of such standards
} would be most helpful, perhaps to others as well.
}
}
} Thanks in advance -
}
}
} Finn-Mogens Haug
}
} Department of anatomy E-mail: f.m.haug-at-basalmed.uio.no
} Institute of basic medical sciences Phone : +47 22 85 12 67
} University of Oslo Fax : +47 22 85 12 78
}
}
} End of returned message
}
}
}


Finn-Mogens Haug

Department of anatomy E-mail: f.m.haug-at-basalmed.uio.no
Institute of basic medical sciences Phone : +47 22 85 12 67
University of Oslo Fax : +47 22 85 12 78





From: Paul Webster :      Paul.Webster-at-QuickMail.Yale.edu
Date: 28 Sep 1995 09:56:12 -0400
Subject: Re: Administrivia

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Message-Id: {n1399840050.2433-at-QuickMail.Yale.edu}

Finn-Mogens Haug writes:

"When registering to this list recently, I probably made some mistake.
The problem is that after submitting a message to the list, I receive
the error messages that arise when members of list are unreachable.
Thus, after submitting two messages I received altogether 35 error
messages."

Reply:

You are not the only one who gets these messages. Every time I submit a
posting, I get many similar messages. I have taken the easiest route which is
to post infrequently and answer other postings directly, not via this forum. I
am sure that many others are taking this option because although questions are
being posted, few of the replies are seen by all.

Additionally, it appears that I do not see all the messages posted here. I know
this, not only because I pick up answers to questions I never saw but also
because I never even saw two messages posted by a colleague. She warned me in
advance that they were being sent and she even received replies from some
subscribers, so they did get posted.

The administrators at my end are mystified by this. Perhaps our administrator
could add some comments. After all, the reason we subscribe is so that we can
freely exchange messages with others of similar interests. If the posting
mechanisms are deterring us from adding our comments then the system may soon
fall apart.

Perhaps this is all a sophisticated technological plan to filter out trivia!

Best regards,
Paul Webster
Yale School of Medicine





From: f.m.haug-at-basalmed.uio.no (Finn-Mogens Haug)
Date: Thu, 28 Sep 1995 18:07:50 +0200
Subject: Thanks. Was: Administrivia, please help

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In answering my question

} } When registering to this list recently, I probably made some mistake.
} } The problem is that after submitting a message to the list, I receive
} } the error messages that arise when members of list are unreachable.
} } Thus, after submitting two messages I received altogether 35 error
} } messages. An example is enclosed at the end here.

most of you replied along the following lines:

}
} There is absolutely nothing wrong with your setup and I believe there is
} very little the site administrator could do to resolve the problem.
} Actually, this is not a "problem" but the way the e-mail system works.
} Since you are the originator of the message, when any of the group
} subscribers moves, changes e-mail address or his/her computer is down, the
} e-mail system will report the fact that your message could not be delivered
} (I believe delivery is tried at least three times before the undeliverable
} mail message is sent back to the originator). So. I am afraid you, as I
} have in the past, have just come in contact with the "dark side" of
} subscribing to an interest group.
}

I have only registered with two other lists (and some news-groups) before
this one and there it looks as if the liststservers filter out error messages
of this kind.

} I still think the gains outweigh the losses.
}

I will stay tuned. Thanks for your help.


Finn-Mogens


Finn-Mogens Haug

Department of anatomy E-mail: f.m.haug-at-basalmed.uio.no
Institute of basic medical sciences Phone : +47 22 85 12 67
University of Oslo Fax : +47 22 85 12 78





From: W.L. Steffens :      STEFFENS.B-at-calc.vet.uga.edu
Date: Thu, 28 Sep 1995 12:48:42 EST
Subject: Administrivia

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Having a mail reader with mail-filtering capability really helps with the
returned mail overload after posting to this and any other list. The
elaborate filtering system that I have set up in my reader (Pegasus Mail)
has virtually eliminated the "Undeliverable Mail", "Returned Mail", etc.
as well as the equally aggrevating "Subscribe", "Unsubscribe" postings.
It's even useful to filter out postings from certain individuals that you
have grown weary of. (Not on this list of course!)

-=W.L. Steffens=-
Department of Veterinary Pathology
College of Veterinary Medicine
University of Georgia




From: Doug Davis :      doug_davis-at-maillink.berkeley.edu
Date: 28 Sep 1995 10:18:05 -0700
Subject: Freeze-fracture manual

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Message-ID: {n1399839393.82774-at-maillink.berkeley.edu}

Subject: Time: 10:13 AM
OFFICE MEMO Freeze-fracture manual Date: 9/28/95

Dear subscribers:
We are in need of a copy of the operations manual for the JEOL JFD-9000.

Contact: Paula Sicurello at (510) 642-2085
paula_sicurello-at-mailink.berkeley.edu

Thank you.





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-aaem.amc.anl.gov
Date: Thu, 28 Sep 1995 12:23:49 -0500 (CDT)
Subject: Missing Messages & Administrivia

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G'day Colleagues

Yes I know about the periodic Undeliverable Mail problems.
I now (finally) have some new software which I am in the process of
installing/debugging. If all goes well in the next few weeks
the system will get better. I keep a reasonable eye on things
and when I notice multiple errors from the same site (or people
tell me about it) then I remove that address from the subscription
list.

But as most of you know it's getting harder and harder
to clone more copies of myself. The first five copies were reasonable,
but now they have to grow up, although a few of them are begining to
show some promise.;-)


I noticed Paul's report of mail not getting through.
I need to know this type of information so if you have specifics
then forward them to me.

Your Friendly Neighborhood SysOp

Nestor

P.S. I do get alot of Email (50+/day), so don't be offended if I don't
reply to everything. I do need to do other things occassionally (like
sleep....).








From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 28 Sep 1995 15:00:04 -0400
Subject: RE-FrzFrctrManual

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Message-ID: {n1399822036.88806-at-mse.engin.umich.edu}

Subject: Time: 2:45 PM
OFFICE MEMO RE:FrzFrctrManual Date: 9/28/95

There is a rather detailed discussion of the operation of the vacuum system
of the JEOL 9010 Freeze-Fracture apparatus in Section 9.1, pp. 373 - 381, of
my book "Vacuum Methods in Electron Microscopy" (available from Ashgate
Publishers, Old Post Road, Brookfield, VT 05036-9704, Ph: 800-535-9544, Fx:
802-276-3837). Perhaps this would be helpful to you, since, apart from
specimen preparation, manipulation of the vacuum system is a major part of
the operation of the instrument.
Good luck, W. C. Bigelow (bigelow-at-umich.edu)





From: dlb-at-u.Arizona.EDU (David Bentley)
Date: Thu, 28 Sep 1995 14:08:12 -0700
Subject: Re: Administrivia

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Sorry for jumping in, but I did want to make a comment on this
topic. Despite the extra traffic due to undeliverable mail warnings(Yes, I
get them too), I would like to encourage members of the list to respond to
the list as opposed to the individule, as often as possible. Many of the
questions asked, pertain to topics, I and others are interested in, and our
questions haven't quite gelled yet, so the answer is informative to many of
us. Further more, some of the information offered, provides different ways
of accomplishing tasks that get tucked away in the "I have to try that
someday" file (the Hans Ris "sticky slide" response is a good example). In
a lot of the replies, even if it was the same as I might have offered is a
different perspective that helps me to understand various processes better.
Sometimes, in a reply, there is just a word or two that provides a solution
to a problem. Lastly, some of the questions that I think I have the answer
for, the alternatives offered here were solutions, I never would have
thought of.
From my selfish point-of view, I hope many will answer questions on
the listserver for all to view, even though there will be those 30 or so
undeliverable notes tomorrow to muddle through. I also thought I had made a
mistake when I got a number of undeliverable mail warnings from my first
reply, but talking to the system administrator calmed me down and as in
another response, he was surprised there was so few for a listserver this size.
Thanks to all who take the time to respond to our questions.
later dlb

David Bentley
Imaging Facility, Div. of Biotechnology, ARL
The University of Arizona
Tucson, Az 85721





From: emccjb-at-ttuhsc.edu (Charles J. Butterick)
Date: Thu, 28 Sep 1995 14:08:12 -0700
Subject: Resolution vs. magnification

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Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
To: MICROSCOPY-at-AAEM.AMC.ANL.GOV

Greetings,
I'm having an energetic discussion with a photographer on the
proper way to indicate the true magnification on a micrograph. One denotes
the print magnification by the negative magnification only. The other
denotes a print magnification as the negative magnification times the
enlargement factor. The former believes that no increased magnification
exists unless there is a concommittant increase in resolution and that it
is misleading to do otherwise. The latter believes that magnification can
be increased (without necessarily increasing resolution) by enlargement and
that total resultant magnification should be used to describe the
micrograph; accurate measurements on the print being impossible otherwise.
Anybody care to wade into the discussion?



Charles J. Butterick (Chuck)
Electron Microscopy Center
Department of Cell Biology
and Biochemistry
Texas Tech University Health
Sciences Center
3601 4th Street
Lubbock, Texas 79430

vox (806) 743-1633
fax (806) 743-1219
email emccjb-at-ttuhsc.edu or
chuck-at-micron1.lubb.ttuhsc.edu






From: Peter D. Barnett :      pbarnett-at-crl.com
Date: Thu, 28 Sep 1995 21:24:09 -0700 (PDT)
Subject: Resolution vs. magnification

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To state the obvious - the two are different. Which on is expressed
depends on what you want to know. Most of the time when I present
photomicrographs the relevant issue is "how much smaller is the actual
thing than what is shown in the picture." This is obviously a
magnification issue on the image that the viewer is actually seeing.

The problem does become more fun when projecting a slide. Does someone in
the audience really have a good idea of how large the projected image is?
Doesn't it depend on where in the audience the person sits?

I suppose the best general solution is to include a good scale in the
original preparation. I don't consider a 1 micrometer bar a good scale
for most folks.


Peter D. Barnett - Forensic Science Associates - Richmond CA
pbarnett-at-crl.com VOICE: 510-222-8883 FAX: 510-222-8887






From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-aaem.amc.anl.gov
Date: Thu, 28 Sep 1995 23:14:37 -0500 (CDT)
Subject: Magnification Opinion

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Why bother with magnification at all. I always simply
place a "micron" (or nanometer etc..) marker on the image.
In this way the image is always calibrated regardless of
what anyone else does to the print after you give it to them.

Nestor
Your Friendly Neighborhood SysOp




From: Stefan.Gunnarsson-at-devbiol.uu.se (Stefan Gunnarsson)
Date: Fri, 29 Sep 1995 08:17:22 +0100
Subject: Re:Resolution vs. magnification

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X-Sender: zoogun-at-strix.udac.uu.se
Message-Id: {v01510101ac9149de3c4d-at-[130.238.80.10]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Chuck writes:


} I'm having an energetic discussion with a photographer on the
} proper way to indicate the true magnification on a micrograph. One denotes
} the print magnification by the negative magnification only. The other
} denotes a print magnification as the negative magnification times the
} enlargement factor. The former believes that no increased magnification
} exists unless there is a concommittant increase in resolution and that it
} is misleading to do otherwise. The latter believes that magnification can
} be increased (without necessarily increasing resolution) by enlargement and
} that total resultant magnification should be used to describe the
} micrograph; accurate measurements on the print being impossible otherwise.
} Anybody care to wade into the discussion?
}
}


Of course the print magnification can and should be used. I know that there
is no way to increase the information contents of an image by magnifying
it, but it is of no help to me when I see a print to know that the negative
magnification was this or that. A scale bar included in the print is I
think the best solution. Then it doesn't matter if the size of the image is
changed in the publishing process, and anyone who so wishes can easily
determine the resolving power of the microscope or whatever is used for the
imaging of the specimen.

Stefan


..............................................................................

Stefan Gunnarsson
Microscopy Unit,Dept. of Animal Development and Genetics

Uppsala University
Norbyv. 18A, S-75236 UPPSALA, Sweden
e-mail Stefan.Gunnarsson-at-devbiol.uu.se








From: Stefan.Gunnarsson-at-devbiol.uu.se (Stefan Gunnarsson)
Date: Fri, 29 Sep 1995 08:24:56 +0100
Subject: Re: Administrivia

Contents Retrieved from Microscopy Listserver Archives
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X-Sender: zoogun-at-strix.udac.uu.se
Message-Id: {v01510102ac914f498214-at-[130.238.80.10]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

David Bentley wrote

} Sorry for jumping in, but I did want to make a comment on this
} topic. Despite the extra traffic due to undeliverable mail warnings(Yes, I
} get them too), I would like to encourage members of the list to respond to
} the list as opposed to the individule, as often as possible. Many of the
} questions asked, pertain to topics, I and others are interested in, and our
} questions haven't quite gelled yet, so the answer is informative to many of
} us. Further more, some of the information offered, provides different ways
} of accomplishing tasks that get tucked away in the "I have to try that
} someday" file (the Hans Ris "sticky slide" response is a good example). In
} a lot of the replies, even if it was the same as I might have offered is a
} different perspective that helps me to understand various processes better.
} Sometimes, in a reply, there is just a word or two that provides a solution
} to a problem. Lastly, some of the questions that I think I have the answer
} for, the alternatives offered here were solutions, I never would have
} thought of.
} From my selfish point-of view, I hope many will answer questions on
} the listserver for all to view, even though there will be those 30 or so
} undeliverable notes tomorrow to muddle through. I also thought I had made a
} mistake when I got a number of undeliverable mail warnings from my first
} reply, but talking to the system administrator calmed me down and as in
} another response, he was surprised there was so few for a listserver this size.
} Thanks to all who take the time to respond to our questions.
} later dlb
}

All I want to say is:

Hear, hear!


..............................................................................

Stefan Gunnarsson
Microscopy Unit,Dept. of Animal Development and Genetics

Uppsala University
Norbyv. 18A, S-75236 UPPSALA, Sweden
e-mail Stefan.Gunnarsson-at-devbiol.uu.se








From: Yves Maniette :      yves-at-giga.sct.ub.es
Date: Fri, 29 Sep 1995 11:51:16 +0000
Subject: Re: unread messages

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}
} Additionally, it appears that I do not see all the messages posted here.
I know
} this, not only because I pick up answers to questions I never saw but also
} because I never even saw two messages posted by a colleague. She warned me in
} advance that they were being sent and she even received replies from some
} subscribers, so they did get posted.
}
} The administrators at my end are mystified by this. Perhaps our administrator
} could add some comments. After all, the reason we subscribe is so that we can
} freely exchange messages with others of similar interests. If the posting
} mechanisms are deterring us from adding our comments then the system may soon
} fall apart.

I may have one answer to this point. I used a few months ago a soft
called POPMAIL, and actually there WERE messages that never went through.
Then once I could notice that all of these undetected mail had one commun
feature (by looking directly in the inbox.mbx directory):
There was always one blank line in the header: for any cryptic reason the
soft was unable to recognise them as mails. However the mails files did
exist, and by removing this blank line (using the EDIT program of MS DOS
for example) I could get them back to life. I have now switched to PINE
and believe things are far easier. Which mail system are you using?


Yves MANIETTE




From: f.lawrence-at-qut.edu.au (Felicity Lawrence)
Date: Fri, 29 Sep 1995 14:55:38 +1000
Subject: American Society for Cell Biology

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G'day.

I'm looking for information regarding the American Society for Cell Biology.
Could someone please tell me who to contact to get registration and
accomodation details?

Thanks,
Felicity
EM Unit, QUT. Brisbane, Australia





From: Mark Aindow :      aindowm-at-sun1.bham.ac.uk
Date: Fri, 29 Sep 1995 12:23:15 +0100
Subject: TEM/STEM postdoctoral position

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Advertisment forwarded to the microscopy mail reflector on behalf
of a colleague. Please address all enquiries to the numbers and
addresses listed below - not to me thanks, Mark Aindow

***************************************************************************

Postdoctoral Position Available at The University of Birmingham,
IRC in Materials for High Performance Applications, Birmingham, UK

Synthesis of Oxide Ceramic Precursor Sols for Structural and
Functional Ceramics


A Research Fellow (RA1A grade) is required to work on the hydrothermal
synthesis and emulsion synthesis of ultrafine nanometre size oxide ceramic
precursor sols for structural and functional ceramics with the desired
chemical stoichiometry, phase composition and homogeneity. The compositions
will be chosen as being representative of single-, dual-, and multi-cation
oxide ceramics, and will need to be characterized physically, structurally
and chemically using advanced analytical techniques such as TEM/EDX, XRD,
PEELS etc.

In the first instance this is a two-year EPSRC funded core project in the
IRC in Materials for High Performance Applications, The University of
Birmingham. There is the possibility of a project extension. Thus, the
initial appointment will be for 2 years from December 1995 to December
1997. Starting salary will be in the range of 14,317 to 17,446 pounds
sterling per annum depending on relevant ability and expertise.

Preliminary enquiries and requests for further particulars should be
directed to Dr. C.B. Ponton by telephone on + 44 -121-414 5226;
FAX on +44-121-414-3441, or preferably by Email to C.B.Ponton-at-bham.ac.uk

The post would suit ideally a person who could meet all the following
essential criteria; and at least some, if not all of the desirable
criteria.

Essential :

A good working knowledge of transmission and scanning electron beam
techniques, particularly TEM and/or STEM chemical analysis (EDX) and
structure determination techniques is essential.

The ability and desire to extend undergraduate and/or graduate knowledge of
aqueous and/or organo-aqueous reaction chemistry to the hydrothermal and
emulsion synthesis of ceramic precursor sols, and to develop hydrothermal,
i.e. high temperature and pressure process engineering skills.

Proven ability to carry out scientific research and to communicate the
results of the research by scientific journal publications and conference
presentations.

Ability to work in a team and interact with workers in other
scientific/engineering disciplines.

Computer literate as regards computer-based word-processing, data logging
and analysis.

Desirable :

Experience of applying electron beam chemical analysis techniques such as
TEM and/or STEM EDX and (P)EELS with ELNES applied to nanometre size
ceramic particulates.

Familiarity with advanced chemical and spectroscopic characterisation
techniques including quantitative powder XRD and one or more of MAS MRI,
FTIR etc.

Familiarity with inorganic and organometallic reaction chemistry including
reaction thermodynamics and kinetics as well as ceramic powder processing
science and technology including powder pressing, sintering and mechanical
property testing.





From: L.S. SMITH :      MTLLSS-at-ECU-01.NOVELL.LEEDS.AC.UK
Date: Fri, 29 Sep 1995 13:49:59 GMT
Subject: Structure of Pyrochlore

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Does anybody have any crystallographic information, or know of a
source, of lead lanthanum titanate pyrochlore phase. This phase is
often refered to in the literature, however aquiring precise
structural data has proved difficult.
Thank,
***************************************
************ Lee Smith ************
******* School of Materials *******
******* University of Leeds *******
******* Leeds, LS2 9JT, UK *******
***************************************




From: EMLAB-at-opus.mco.edu
Date: Fri, 29 Sep 1995 08:49:12 -0500 (EST)
Subject: Re: Resolution vs. magnification

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Hi Chuck,

I always consider the print magnification to be the neg. mag times
the enlargement factor. The resolution should only be calculated from a
neg. therefore resolution is mostly mote when looking at a print.(except when
publishing).

Best wishes,
Ed Calomeni
Medical College of Ohio
Toledo, OH
emlab-at-opus.mco.edu




From: Dirk Knoesen, UWC, SA :      DIRK-at-physics.uwc.ac.za
Date: 29 Sep 95 14:42:37 GMT+0200
Subject: COMMENT: Resolution vs. magnification

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Message-ID: {MAILQUEUE-101.950929144237.384-at-physics.uwc.ac.za}
To: microscopy-at-aaem.amc.anl.gov

Hi

Just my two cents worth of knowledge:

Magnification on the print is the total magnification, ie
negative magnification times enlargement factor. It might
be that it is an empty magnification, ie no further detail
becomes visible due to poor resolution on the micrograph.
However the negative own grain structure is usually far
smaller than detail visible (resolved?) on the micrograph,
ie if the micrograph is done well, you should be able to
get additional detail at enlargerment factors up to 10
times.
In either case you do not change anything on the original
resolution of the micrograph, only the visibility thereof
becomes better (that's why you look at a micrograph through
a manifying lens to see the detail on the micrograph not
visible to the naked eye).

Hope it clears up some confusion,
Dirk


} Greetings,
} I'm having an energetic discussion with a photographer on the
} proper way to indicate the true magnification on a micrograph. One denotes
} the print magnification by the negative magnification only. The other
} denotes a print magnification as the negative magnification times the
} enlargement factor. The former believes that no increased magnification
} exists unless there is a concommittant increase in resolution and that it
} is misleading to do otherwise. The latter believes that magnification can
} be increased (without necessarily increasing resolution) by enlargement and
} that total resultant magnification should be used to describe the
} micrograph; accurate measurements on the print being impossible otherwise.
} Anybody care to wade into the discussion?
}
}
}
} Charles J. Butterick (Chuck)
}


Prof Dirk Knoesen U U W W CCCCCC
Department of Physics U U W W C
University of the Western Cape U U W W W C
Private Bag X17, Bellville 7535 U U W W W W C
South Africa UUUUUU W W CCCCCC
Tel:+21 959 2266. Fax:+21 959 3474. Internet: dirk-at-physics.uwc.ac.za




From: SJOSTROM-at-KRDC.INT.ALCAN.CA
Date: Fri, 29 Sep 1995 09:09:49 -0500 (EST)
Subject: Mag vs Res!

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Message-ID: {2BC06B300179AEAA-at-ggpl.arsusda.gov}


--Boundary (ID EukuE+CAP3e/3Urph/Qb5A)
Content-type: TEXT/PLAIN

Working in a metallographic lab and and electron optic lab the best way to
label images with an indicator for subsequent measurement is to use a micron
marker (calibrated of course). Regardless of mag. direct measurements can
then be taken with better accuracy. Enlarge the image and you enlarge the
measuring marker equally as well.

That being said mag. and res. are obviously two different "critters". By
defintion magnification increases an object's size so that we can observe it
but resolution allows us to separate fine detail in that object. Who cares
how large we make an object if we can't resolve any features! Image
size will be increased by enlargement but USEFUL mag. is not, nor is resolution
increased! Take a 4x5 inch 500x print of a certain microstructure and measure
a feature, divide by the mag to get "true" size. Now enlarge the print by 4
times, remeasure the feature and divide by the same mag. What happens? Your
numbers don't match! True if you include the enlargment factor, the numbers
will match but you have not increased the mag. You have actually used "empty
magnification" to make the object larger.

I think the differences lies in the interpretation of magnifiction.
Microscopists interpret mag as an increased image size that supplies useful
information. I suppose photographers refer to magnification as enlarged
images.

The lesson learned, there is useful mag and empty mag and don't use
magnification as the be all and end all for measurement purposes!

Just a comment.

--Boundary (ID EukuE+CAP3e/3Urph/Qb5A)--




From: RBE :      richard.beanland-at-gecm.com
Date: Fri, 29 Sep 1995 15:06:58 +0000 (GMT)
Subject: TEM: Jet thinning of LiNbO3

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Disclose-Recipients: prohibited

Dear All,
WE ARE INTERESTED IN CHEMICAL ETCHES FOR LiNbO3, to prepare TEM
SPECIMENS BY JET ETCHING. DOES ANYONE HAVE A RECIPE THEY CAN RECOMEND.



Many thanks in advance,


Richard Beanland,
GEC-Marconi Materials Technology Ltd.,
Caswell,
Towcester,
Nothants NN12 8EQ
Tel. (01327) 356363
Fax. (01327) 356775
Email rbe-at-gecm.com






From: Jay Jerome :      jjerome-at-isnet.is.wfu.edu
Date: Fri, 29 Sep 1995 10:22:56 -0400 (EDT)
Subject: Re: Resolution vs. magnification

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On Thu, 28 Sep 1995, Charles J. Butterick wrote:

} Greetings,
} I'm having an energetic discussion with a photographer on the
} proper way to indicate the true magnification on a micrograph. One denotes
} the print magnification by the negative magnification only. The other
} denotes a print magnification as the negative magnification times the
} enlargement factor. The former believes that no increased magnification
} exists unless there is a concommittant increase in resolution and that it
} is misleading to do otherwise. The latter believes that magnification can
} be increased (without necessarily increasing resolution) by enlargement and
} that total resultant magnification should be used to describe the
} micrograph; accurate measurements on the print being impossible otherwise.
} Anybody care to wade into the discussion?
}


Magnification is magnification and resolution is resolution and never the
twain shall meet. The negative magnification can be just as empty
magnification as the print magnification. IT IS IMPERATIVE TO GIVE THE
MAGNIFICATION OF THE "IMAGE" THE VIEWER IS LOOKING AT. As you pointed out
this is the only way for readers to make measurements from the "image"
they have in front of them. I don't submit manuscripts to journals which
have the nasty habit of changing the size of micrographs and including in
the caption something like "reduced from the original magnification of
X". This is a meaningless phrase. It gives me no information except the
warning not to trust the image. Even if the image is pointlessly
magnified above the effective resolution, you still need the
magnification information to judge the science.

The other alternative is to include a measuring standard bar in the
image. This avoids all such problems.

That is my two cents worth. Obviously, this is something I feel very
strongly about.

Thanks for listening-


Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-isnet.is.wfu.edu *
**************************************************************










From: William Tivol :      tivol-at-wadsworth.org
Date: Fri, 29 Sep 1995 10:46:46 -0400 (EDT)
Subject: Re: Resolution vs. magnification

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Dear Charles,
I, too, go along with the use of a scale bar to indicate magnification
(could this agreement among the members of this list be why MSA asks for scale
bars :-)). There is, however, a reason that the neg mag and print enlarge-
ment factors could be useful in some circumstances. For high mags and largish
grain, these numbers could tell one how much graininess is due to the film and
how much is due to noise. In this case, it is the neg mag which is important,
and the scale bar alone is not sufficient.
Yours,
Bill Tivol




From: Adriana Pinheiro Martinelli Rodriguez :      adriana-at-aguia.cena.usp.br
Date: Fri, 29 Sep 1995 11:14:27 -0200 (BDT)
Subject: Re: American Society for Cell Biology

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Posted-Date: Fri, 29 Sep 1995 11:14:27 -0200 (BDT)
Received-Date: Fri, 29 Sep 95 11:14:27 BDT

Hello:
I will forward to you a message that I received from Ms. Kathy King about
the Annual Meeting of the American Society for Cell Biology. I hope
that's what you are looking for.

Adriana Rodriguez
Secao de Biologia e Melhoramento Vegetal
Centro de Energia Nuclear na Agricultura
Universidade de Sao Paulo, Brazil

On Fri, 29 Sep 1995, Felicity Lawrence wrote:

} G'day.
}
} I'm looking for information regarding the American Society for Cell Biology.
} Could someone please tell me who to contact to get registration and
} accomodation details?
}
} Thanks,
} Felicity
} EM Unit, QUT. Brisbane, Australia
}
}




From: Bob_Lawrence-A402AA-at-email.sps.mot.com
Date: 29 Sep 95 10:28:36 -0500
Subject: Res/Mag hoopla

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REGARDING Res/Mag hoopla
To the group,

This is a test of my mail system response and a short comment on the ongoing
discussion. It seems perfectly obvious that if one can't see what is being
blown up in the print, it is useless. I don't know anyone that makes prints of
things that can't be seen! If one can see it in the print magnification is
always useful as it gives one the scale, if not why make the print. I am a
failure analyst and an amateur photographer, I'm somewhat baffled by the logic
of this discussion.

Have a very nice day. I am enjoying the mail I receive from this listserver
and I hope in the future to contribute something more useful than this comment.

Respectfully,
Bob Lawrence





From: wise-at-VAXA.CIS.UWOSH.EDU
Date: Fri, 29 Sep 1995 10:42:51 +0000
Subject: Res/Mag hoopla

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To all,

Thanks for the many replies to my query regarding Polaroid
processing buckets ("PN-10; Clearing Tank
For 665/55 Film", for something like $39.95, this includes the tank, the
rack and a pound of sodium sulfite). Several people suggested contacting
Graphic Center, P.O. Box 818, Ventura, CA. 93002. Phone : 1-800-336-6096.
Others said that Polaroid stopped making them several years ago and they
are unavailable. I called the Graphic Center and their tape machine said
they will be closed from Sept 28-Oct 8 (maybe they all took a road trip to
pick up a new supply of PN-10s). I'll try them again after the 8th and let
ya'll know if I am successful.

Bob


Robert R. Wise, PhD
Director, UWO Electron Microscope Facility
Department of Biology
University of Wisconsin Oshkosh
Oshkosh, WI 54901
(414) 424-3404 tel
(414) 424-1101 fax
wise-at-vaxa.cis.uwosh.edu






From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Fri, 29 Sep 1995 10:36:00 -0500
Subject: Magnification and scale bars

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Message-Id: {199509291537.KAA09711-at-bcm.tmc.edu}
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X-Mailer: Windows Eudora Version 2.1.1
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

The magnification vs. resolution multilog has been interesting and many have
extolled the virtues of scale bars. Let me point out that scale bars are not
as appropriate for those images having a significant depth demension. An
example might be a photograph of you ten feet from the camera with the
Washington Monument visible five miles away in the background. A scale bar
would not be able to indicate both your size and the size of the monument.
Scanning electron micrographs (secondary electron imaging) are such images
and it is best to express the magnification for these numerically.


Joiner Cartwright, Jr., Ph.D.
Director, Electron Microscopy
Department of Pathology, Rm.286-A
Baylor College of Medicine
One Baylor Plaza
Houston, Texas 77030 U.S.A.
tel.: (713)798-4658
FAX: (713)798-3945
joiner-at-bcm.tmc.edu
Compuserve: 71555,1206





From: A. Kent Christensen :      akc-at-umich.edu
Date: Fri, 29 Sep 1995 11:38:21 -0400 (EDT)
Subject: Re: Resolution vs. magnification

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Resolution is how much detail your system was able to put into the
micrograph. The optimal resolution of a system is usually defined by the
familiar equation, R=(0.61&)/(u sin -at-).

Magnification is how much the original object has been enlarged,
usually negative magnification times enlargement factor. Your negative
magnification, of course, is how many times larger the structures shown
in your negative are compared to the original structures you were
photographing (or "electrographing"). A micron bar is useful, since it
shows how long a micron would be in terms of the original structure.

Further magnification of a negative can make the detail more easily
visible, but it can't create new detail (in other words, it can't
increase the resolution of the original negative).

A. Kent Christensen, University of Michigan, {akc-at-umich.edu}

---------------------------------------

On Thu, 28 Sep 1995, Charles J. Butterick wrote:

} Greetings,
} I'm having an energetic discussion with a photographer on the
} proper way to indicate the true magnification on a micrograph. One denotes
} the print magnification by the negative magnification only. The other
} denotes a print magnification as the negative magnification times the
} enlargement factor. The former believes that no increased magnification
} exists unless there is a concommittant increase in resolution and that it
} is misleading to do otherwise. The latter believes that magnification can
} be increased (without necessarily increasing resolution) by enlargement and
} that total resultant magnification should be used to describe the
} micrograph; accurate measurements on the print being impossible otherwise.
} Anybody care to wade into the discussion?
}
}
}
} Charles J. Butterick (Chuck)
} Electron Microscopy Center
} Department of Cell Biology
} and Biochemistry
} Texas Tech University Health
} Sciences Center
} 3601 4th Street
} Lubbock, Texas 79430
}
} vox (806) 743-1633
} fax (806) 743-1219
} email emccjb-at-ttuhsc.edu or
} chuck-at-micron1.lubb.ttuhsc.edu
}
}
}




From: krogers-at-ecn.purdue.edu (kirk rogers)
Date: Fri, 29 Sep 1995 10:50:18 -1812
Subject: Re: unread messages

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Message-Id: {v01530501ac927c9f5d40-at-[128.46.155.237]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Yves,

PINE is an exceptional mail reader for UNIX, but there are much more
intuitive and more easily configurable mail readers available in Macintosh
and Windows format.

I currently use the Eudora (available on both formats) from QUEST. There
is a freeware version and a commercial version. Both use TCP/IP software
to interface with the POP mail server and make replying, editing, building
group mail aliases, and sending attached files easy! They both provide the
capability for multi-user use from the same machine (currently 7 people use
this copy - each with separate preferences) as well as the capability to
have multiple mailboxes. The commercial version will sort (or block) mail
by subject or kewords in the body of the mail message and place it in the
proper mailbox and do a couple more things.

More info can be found via the following:

QUALCOMM Enterprise Software Technologies (QUEST) Sales Administration at
(800) 2-Eudora, (619) 658-1291,

OR quest-rep-at-qualcomm.com.

OR ftp.qualcomm.com/quest/product_literature

OR http://www.qualcomm.com/quest

-Kirk

________________________________________________
Kirk A. Rogers
krogers-at-materials.ecn.purdue.edu
317-494-8751 office http://materials.ecn.purdue.edu/~krogers
Purdue Unuversity, School of Materials Engineering,
1289 MSEE building, W. Lafayette, IN 47907-1289






From: Bob_Lawrence-A402AA-at-email.sps.mot.com
Date: 29 Sep 95 10:28:36 -0500
Subject: Res/Mag hoopla

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microscopy-at-aaem.amc.anl.gov
X-Mailer: Mail*Link SMTP-QM 3.0.2

RE} Res/Mag hoopla 9/29/95

I for one prefer not to put markers in my images, as I believe they distract
from the art
aesthetics.

--------------------------------------

REGARDING Res/Mag hoopla
To the group,

This is a test of my mail system response and a short comment on the
ongoing
discussion. It seems perfectly obvious that if one can't see what is being
blown up in the print, it is useless. I don't know anyone that makes prints
of
things that can't be seen! If one can see it in the print magnification is
always useful as it gives one the scale, if not why make the print. I am a
failure analyst and an amateur photographer, I'm somewhat baffled by the logic

of this discussion.

Have a very nice day. I am enjoying the mail I receive from this
listserver
and I hope in the future to contribute something more useful than this
comment.

Respectfully,
Bob Lawrence


------------------ RFC822 Header Follows ------------------
Received: by qmgate_backup.anl.gov with SMTP;29 Sep 1995 10:35:37 -0500







From: James S MArtin :      James.S.Martin-at-williams.edu
Date: Fri, 29 Sep 1995 13:34:00 -0400 (EDT)
Subject: re: magnification vs. resolution

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As a tangent to the current thread on magnification and resolution, our
photographer today inquired about the difference between a macrograph
(e.g., a photograph made using a macro lens) and a micrograph. Anyone
want to jump in on this?

James Martin




From: RYAN-at-hws.edu
Date: Fri, 29 Sep 1995 13:49:23 -0400 (EDT)
Subject: shutter controller

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Can anyone recommend a decent shutter crontroller for a Zeiss Axiovert
microscope that has epi-fluor and DIC? Hopefully one that is not too
expensive and manually operated. Thanks,
Jim Ryan
Biology Department
Hobart and William Smith Coleges
Geneva, NY 14456
ryan-at-hws.edu




From: VETO-at-BCRSSU.AGR.CA
Date: 29 Sep 1995 14:25:51 -0400 (EDT)
Subject: Resolution vs. magnifications

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Hello,

There is often confusion about resolution and magnification. One can produce
very high magnification of the image by the enlargement factor, without
increasing resolution. (fuzzy, unsharp...) The resolution mainly depends
on the image forming instrument (LM, TEM, SEM, AFM...) AND the specimen,
the enlarger is only a tool to provide additional magnification for the
human eye (it has limits also!) to view the detail of the image. There are
many other factors influence the resolution of the image, many excellent
books have been published on this subject.

To indicate the total or final magnification (enlargement factor X image
magnification on the negative) on the print, is to apply the appropriate
"micron" marker. The marker also "calibrates" all slides, hard copies and
computer images.

Laszlo J. Veto
Electron Microscopist
Research Centre
Summerland, B.C.
Canada V0H 1Z0
604-494-7711 voice
604-494-0755 fax
Veto-at-bcrssu.agr.ca e-Mail




From: Tina Carvalho :      tina-at-halia.pbrc.Hawaii.Edu
Date: Fri, 29 Sep 1995 08:53:12 -1000 (HST)
Subject: Magnification

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Whoa, people, you must all remember something important when labeling or
viewing images with magnifications bars. That bar is only useful for
measurements *in the plane of the film*! This is a problem with SEM
micrographs. You are looking at a 2-D representation of a 3-D object,
and two structures that may seem to be connected or overlapping or near
each other (due to the effects of foreshortening) may, in fact, be far
apart. WHen training researchers on the SEM I make sure that they
understand this and get used to tilting and rotating the stage a lot to
see the true relationships between structures. I also make sure they
understand that trying to measure a structure or the distance between
structures that are not in the same plane (the plane of the viewing
screen or the micrograph) is bogus. A few examples usually gets the
idea across. Having given the warning, however, I still tell them to
put the micron bar on there, because without it I'm easily lost! The
editors of some journals prefer only the field magnification for SEMs,
which is correct. Micron bars would be great if the audience
understands their limitations.

What do you editors out there think?

Aloha,
Tina

*****************************************
Tina (Weatherby) Carvalho *
Biological Electron Microscope Facility *
University of Hawaii *
(808) 956-6251 *
tina-at-ahi.pbrc.hawaii.edu *
http://www.pbrc.hawaii.edu/bemf/ *
*****************************************





From: RYAN-at-hws.edu
Date: Fri, 29 Sep 1995 15:32:54 -0400 (EDT)
Subject: shutter controller

Contents Retrieved from Microscopy Listserver Archives
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Can anyone recommend a shutter controller for a zeis microscope that has
epi-fluorescence and dic? I would appreciate the names and phone #'s
of companies producing reasonably priced shutters. Thanks
Jim Ryan
Biology Dept.
Hobart & William Smith Colleges
Geneva, NY 14456
ryan-at-hws.edu




From: DDKJoe-at-aol.com
Date: Fri, 29 Sep 1995 15:57:00 -0400
Subject: Re: American Society for Cell...

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Alternate-Recipient: allowed
Auto-Forwarded: prohibited
Content-Return: allowed
Disclose-Recipients: prohibited
Conversion: allowed
Importance: normal
Priority: normal
Sensitivity: Company-Confidential
Microscopy ListServer {MICROSCOPY-at-aaem.amc.anl.gov}
Message-Id: {950929153107.28541-at-cliff.ml.wpafb.af.mil.0}

Felicity,

The American Society of Cell Biology is meeting in Washington, DC from
December 9 - 13th. Their EMail address is ascbinfo-at-ascb.faseb.org. Have a
pleasant visit.

Joe Tabeling
Delaware Diamond Knives




From: Glen Macdonald :      glenmac-at-u.washington.edu
Date: Fri, 29 Sep 1995 13:00:33 -0700 (PDT)
Subject: Re: Eudora

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X-Sender: glenmac-at-homer01.u.washington.edu
Microscopy {microscopy-at-aaem.amc.anl.gov}


Eudora requires a POP server. If your mail server doesn't support that
protocol, like here at University of Washington, then you can't use Eudora.


Glen MacDonald
Hearing Development Laboratories
University of Washington
Seattle, WA 98195-6515
(206)543-8360
glenmac-at-u.washington.edu

On Fri, 29 Sep 1995, kirk rogers wrote:

} Yves,
}
} PINE is an exceptional mail reader for UNIX, but there are much more
} intuitive and more easily configurable mail readers available in Macintosh
} and Windows format.
}
} I currently use the Eudora (available on both formats) from QUEST. There
} is a freeware version and a commercial version. Both use TCP/IP software
} to interface with the POP mail server and make replying, editing, building
} group mail aliases, and sending attached files easy! They both provide the
} capability for multi-user use from the same machine (currently 7 people use
} this copy - each with separate preferences) as well as the capability to
} have multiple mailboxes. The commercial version will sort (or block) mail
} by subject or kewords in the body of the mail message and place it in the
} proper mailbox and do a couple more things.
}
} More info can be found via the following:
}
} QUALCOMM Enterprise Software Technologies (QUEST) Sales Administration at
} (800) 2-Eudora, (619) 658-1291,
}
} OR quest-rep-at-qualcomm.com.
}
} OR ftp.qualcomm.com/quest/product_literature
}
} OR http://www.qualcomm.com/quest
}
} -Kirk
}
} ________________________________________________
} Kirk A. Rogers
} krogers-at-materials.ecn.purdue.edu
} 317-494-8751 office http://materials.ecn.purdue.edu/~krogers
} Purdue Unuversity, School of Materials Engineering,
} 1289 MSEE building, W. Lafayette, IN 47907-1289
}
}
}




From: ramd-at-beta.lanl.gov (Ram Devanathan)
Date: Fri, 29 Sep 1995 14:44:20 -0600
Subject: Magnification and Resolution

Contents Retrieved from Microscopy Listserver Archives
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} Charles J. Butterick (Chuck) wrote:
} I'm having an energetic discussion with a photographer on the
} proper way to indicate the true magnification on a micrograph. One denotes
} the print magnification by the negative magnification only. The other
} denotes a print magnification as the negative magnification times the
} enlargement factor. The former believes that no increased magnification
} exists unless there is a concommittant increase in resolution and that it
} is misleading to do otherwise. The latter believes that magnification can
} be increased (without necessarily increasing resolution) by enlargement and
} that total resultant magnification should be used to describe the
} micrograph; accurate measurements on the print being impossible otherwise.
} Anybody care to wade into the discussion?


Magnification is the ratio of the size of a feature in the image to
that in the original object. This can be increased by repeated
enlargement without improving resolution. (empty magnification)
To make accurate measurements possible, one should include a scale
marker in the image. If there is no scale marker, the total
magnification should be used to describe the print.

Ram Devanathan ramd-at-lanl.gov




From: gwe-at-biotech.ufl.edu (Greg Erdos)
Date: Thu, 28 Sep 1995 13:14:07 GMT
Subject: SEM magnification and other questionable numbers.

Contents Retrieved from Microscopy Listserver Archives
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Having participated in this list since the beginning, I have noted
that there are a lot of small, but good, bits of practical information that
come down the line. We see question like "How do I do....... ? How do I
make......? Where do I find...? etc.

Here in our lab we often ask if anyone can recall what was said a
few weeks back on solving some problem. Often we have forgotten to save the
message or print it out.

We also see the same questions repeated every few months on the list
as new people join and as we older dudes forget.

Therefore we are going to make a concerted effort to archive all the
good ideas and advice we come across on this list. We will add our own
collection to it as we go. And we want your input.

Since it is just as easy to make the archive available to the world as to
keep it to ourselves we want all of you in on it.

So as an experiment (duration undetermined) we will begin editng and
accumulating what we have come to call "Tips & Tricks" for biological
microscopy in a file available over the internet. We hope someone in
materials microscopy will choose to do likewise for their colleagues.

The file is currently in an embryonic stage but given the proper
gestation should grow to be a valuable resource.

If this task is being done at another site, please tell me ASAP so
we don't waste too much time duplicating the efforts of another/.

Feel free to send us material, unsolicited, that you think
appropriate. Either send text via e-mail or give us a URL that can serve as
a link from our page. Include literature citations whenever possible so
that the right person gets the credit.

You can find us at www.biotech.ufl.edu/~emcl. Go down the page and click
the Wizard.

There is not much there yet, so give us some time to get going.

..........and keep those cards and letters coming.


Regards, Greg Erdos
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Greg Erdos Phone: 904-392-1295
Scientific Director,
ICBR Electron Microscopy Core Lab
218 Carr Hall Fax 904-846-0251
University of Florida E-mail: gwe-at-biotech.ufl.edu
Gainesville, FL 32611
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-





From: Marcelle A Gillott :      magem-at-csd.uwm.edu
Date: Fri, 29 Sep 1995 16:32:34 -0500 (CDT)
Subject: Re: Resolution vs. magnification

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Chuck

In any respectable treatise on optics (including photgraphy books)
MAGNIFICATION is defined as image size divided by object size ("size"
is a linear dimension as opposed to area)

therefore the print magnification is the total magnification or the
negative magnification times the enlargement factor

Marcelle Gillott
uwm


On Thu, 28 Sep 1995, Charles J. Butterick wrote:

} Greetings,
} I'm having an energetic discussion with a photographer on the
} proper way to indicate the true magnification on a micrograph. One denotes
} the print magnification by the negative magnification only. The other
} denotes a print magnification as the negative magnification times the
} enlargement factor. The former believes that no increased magnification
} exists unless there is a concommittant increase in resolution and that it
} is misleading to do otherwise. The latter believes that magnification can
} be increased (without necessarily increasing resolution) by enlargement and
} that total resultant magnification should be used to describe the
} micrograph; accurate measurements on the print being impossible otherwise.
} Anybody care to wade into the discussion?
}
}
}
} Charles J. Butterick (Chuck)
} Electron Microscopy Center
} Department of Cell Biology
} and Biochemistry
} Texas Tech University Health
} Sciences Center
} 3601 4th Street
} Lubbock, Texas 79430
}
} vox (806) 743-1633
} fax (806) 743-1219
} email emccjb-at-ttuhsc.edu or
} chuck-at-micron1.lubb.ttuhsc.edu
}
}
}




From: xxie-at-lsuvm.sncc.lsu.edu (Xiaogang Xie)
Date: Fri, 29 Sep 1995 18:12:38 -0600
Subject: Re: Resolution vs. magnification

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Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Mathematically the second way is absolutely correct. In practice,
useing magnification bar is more convenient and convention.
===================================================================
} Greetings,
} I'm having an energetic discussion with a photographer on the
} proper way to indicate the true magnification on a micrograph. One denotes
} the print magnification by the negative magnification only. The other
} denotes a print magnification as the negative magnification times the
} enlargement factor. The former believes that no increased magnification
} exists unless there is a concommittant increase in resolution and that it
} is misleading to do otherwise. The latter believes that magnification can
} be increased (without necessarily increasing resolution) by enlargement and
} that total resultant magnification should be used to describe the
} micrograph; accurate measurements on the print being impossible otherwise.
} Anybody care to wade into the discussion?
}
}
}
} Charles J. Butterick (Chuck)
} Electron Microscopy Center
} Department of Cell Biology
} and Biochemistry
} Texas Tech University Health
} Sciences Center
} 3601 4th Street
} Lubbock, Texas 79430
}
} vox (806) 743-1633
} fax (806) 743-1219
} email emccjb-at-ttuhsc.edu or
} chuck-at-micron1.lubb.ttuhsc.edu
================================================

Xiaogang

****************************************
* Xiaogang Xie *
* Department of Geology and Geophysics *
* Louisiana State University *
* Baton Rouge, LA 70803 *
* Office (504)388-2240 *
* Fax (504)388-2302 *
****************************************






From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Fri, 29 Sep 1995 18:48:40 -0500
Subject: re: magnification vs. resolution

Contents Retrieved from Microscopy Listserver Archives
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Message-Id: {199509292350.SAA16034-at-bcm.tmc.edu}
X-Sender: joiner-at-bcm.tmc.edu
X-Mailer: Windows Eudora Version 2.1.1
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

At 01:34 PM 9/29/95 -0400, you wrote:
}
} As a tangent to the current thread on magnification and resolution, our
} photographer today inquired about the difference between a macrograph
} (e.g., a photograph made using a macro lens) and a micrograph. Anyone
} want to jump in on this?
}
} James Martin
}
}
************
OK, I'll give it a try. I am going to say that a camera with a macro
lens....IF it is set up to record images larger than the photographed
subject (ie. 1:1+).....is equivalent to a microscope with a film-back.
Therefore your photographer's "macrograph" is the same as a micrograph. I
think that macrograph may be a misnomer, or at the most, jargon. Many
photographers use macrography to mean recording images between what a
"standard" camera lens can do and what a close-up lens can do up to an image
ratio of 1:1, ie. not magnifying.

Howzzat?




Joiner Cartwright, Jr., Ph.D.
Director, Electron Microscopy
Department of Pathology, Rm.286-A
Baylor College of Medicine
One Baylor Plaza
Houston, Texas 77030 U.S.A.
tel.: (713)798-4658
FAX: (713)798-3945
joiner-at-bcm.tmc.edu
Compuserve: 71555,1206





From: KATHY KING :      KATHY-at-ASCB.faseb.org
Date: 20 Sep 95 08:21:00 EDT
Subject: Re: American Society for Cell Biology

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Message-Id: {199509301540.LAA06149-at-post-ofc02.srv.cis.pitt.edu}
To: "microscopy-at-AAEM.AMC.ANL.GOV" {microscopy-at-AAEM.AMC.ANL.GOV}

Felicity:
Here is the information I received from Ms. Kathy King about hotel
reservation and meeting program. I hope that helps
Adriana Rodriguez
BMV/CENA/University of Sao Paulo, Brazil





Follows is hotel information and a summary of the meeting. Let me know if
you need additional information.
-Kathy
================
PARTICIPATING HOTELS AND HOTEL RESERVATION INSTRUCTIONS

=============================
PARTICIPATING HOTELS

RATES (All rates subject to 13% city sales tax and
$1.50 per room/per night occupancy tax)

HOTEL, #ROOMS RESERVED FOR ASCB, SINGLE, DOUBLE,
TRIPLE, CLOSEST METRO STOP*

1. Capital Hilton, 200, $115, $130, $145, Farragut
North (2 blocks), McPherson Square (2 blocks)

2. Comfort Inn Downtown, 150, $175, $175, $175, Gallery
Place (2 blocks), Judiciary Square (4 blocks)

3. Days Inn Downtown, 135, $175, $175, $175, Metro
Center (3 blocks)

4. Doubletree Hotel Park Terrace, 100, $189, $189,
$199, Dupont Circle (3 blocks)

5. Dupont Plaza Hotel, 150, $175, $187, $107, Dupont
Circle (1/2 block)

6. Embassy Square Suites, 150, $179, $189, $129, Dupont
Circle (1 1/2 blocks), (continental breakfast included)

7. Governor s House, 100, $180, $180, $190, Farragut
North (4 blocks)

8. Grand Hyatt Washington, 500, $129, $145, $145, Metro
Center (Hotel is across the street from Convention
Center)

9. Henley Park Hotel, 50, $115, $115, $125, Metro
Center (3 1/2 blocks)

10. Holiday Inn Franklin Square, 150, $169, $169, $179,
McPherson Square (3 blocks)

11. Hotel Washington, 125, $108, $108, $126, Metro
Center (2 1/2 blocks)

12. Marriott at Metro Center, 250, $107, $107, $107,
Metro Center

13. Ramada Plaza, 175, $175, $185, $195, McPherson
Square (3 blocks)

14. Renaissance Washington DC Hotel, 700, $108, $128,
$148, Metro Center (Hotel is across the street from
Convention Center) [Club rates - Single $128, Double
$148, Triple $168 (continental breakfast, and other
amenities included in Club rate)]

*Convention Center is located one block from the Metro
Center stop at the 11th & G Streets exit.

========================

HOTEL RESERVATION INSTRUCTIONS

To make hotel reservations call: 1-800-535-3336 (US &
Canada) or 1-202-842-2930 (Washington, DC &
International)

DEPOSIT: A $100/per room deposit is required for all
reservations. The deposit amount is payable by credit
card or check.

CREDIT CARD: Your credit card will be charged
immediately. Most major credit cards are accepted. Your
room confirmation will be sent upon acceptance of your
credit card charge.

CHECKS: An invoice for the $100/per room deposit will
be mailed to you. Payment must be received within 15
days of the invoice date or your reservation will be
cancelled. Do not send payment without an invoice stub.
Your room confirmation will be sent upon receipt of
your check.

CHANGES: Prior to November 10, all changes should be
made through the ASCB housing service. After November
10, changes should be made directly with the hotel.

CANCELLATIONS/REFUNDS: Cancellations made prior to
November 10 should be made through the ASCB housing
service and will be refunded in full by the ASCB
housing service. Cancellations made after November 10
and PRIOR to 72 hours before arrival should be made
through the hotel which will issue the refund. A $10
fee will be deducted from your deposit.

Reservations must be cancelled 72 hours prior to
arrival or the entire $100 deposit is forfeited.

Please have the following information available prior
to calling for reservations:

1. Name of convention: ASCB
2. 1st, 2nd, and 3rd choice of hotel
3. Arrival/departure dates
4. Number of rooms requested
5. Type of room (single, double, twin, etc.)
6. Number of persons in party
7. Arrival time
8. Credit card type, account number, and expiration
date
9. Names of all occupants of room
10. Address to which confirmation is to be sent
11. Telephone number
12. Fax number (if you would like a confirmation faxed)

INQUIRIES:
ASCB Housing Service
1212 New York Avenue, NW
6th Floor
Washington, DC 20005

International participants may make reservations by
faxing the information requested above to Convention
Housing s 24-hour fax number at 202-289-8079. A written
confirmation will be sent. Please be sure to include a
fax number.
=====================
AMERICAN SOCIETY FOR CELL BIOLOGY
THIRTY-FIFTH ANNUAL MEETING PROGRAM SUMMARY
Saturday, December 9 Wednesday, December 13, 1995
Washington Convention Center
Washington, DC

SATURDAY, DECEMBER 9
11:00AM - 8:00PM Registration
2:00PM - 4:00PM Educating the Public: Ideas for
Scientists as Advocates
1:00PM - 5:00PM Special Interest Subgroup Meetings
3:00AM - 5:00PM Education/Minorities Affairs
Information Booth
6:00PM - 7:00PM PUBLIC POLICY ADDRESS
6:30PM - 9:00PM Posters on Display
7:00PM - 8:00PM SCIENCE KEYNOTE
Eric Kandel, Columbia University,
Genes, Synapses, and the Cell
Biology of Long-Term Memory
8:00PM - 10:00PM Reception
8:00PM - 10:00PM Student Reception


SUNDAY, DECEMBER 10
7:00AM - 8:00PM Congressional Liaison Committee
Breakfast (date and time subject to
change, see official Program)
7:00AM - 6:00PM Exhibitor Showcase
7:30AM - 5:00PM Registration
7:30AM - 9:00PM Posters on Display
8:00AM - 9:30AM SYMPOSIUM I
The Cell Cycle and Cancer
Leland Hartwell, University of
Washington (Chair), Cell cycle
checkpoints
Kim Nasmyth, Institute of Molecular
Pathology, Vienna, Cyclin-dependent
kinases and the cell cycle
Carol Greider, Cold Spring Harbor
Laboratory, Telomerases in cell
immortalization and cancer
9:00AM - 4:00PM Exhibits Open
9:00AM - 5:00PM Education/Minorities Affairs
Information Booth
9:30AM - 10:30AM Complimentary coffee and cookies in
Exhibit Hall
9:45AM - 10:15AM Education Coffee Break Forum
10:30AM - 12:00PM SYMPOSIUM II
Pattern Formation and Evolution
Cynthia Kenyon, University of
California, San Francisco (Chair),
Pattern formation in C. elegans
Sean Carroll, University of
Wisconsin/HHMI, Evolution and
development of the insect body plan
Cliff Tabin, Harvard Medical
School, Evolution and development
of the vertebrate body plan
11:30AM - 3:00PM Minorities Poster Session and
Awards
12:00PM - 3:00PM Poster Presentations
12:30PM - 3:00PM High School Program
2:00PM - 3:00PM E.E. Just Lecture
3:00PM - 3:30PM Refreshment break in Exhibit Hall
3:30PM - 5:45PM Minisymposia 1-6
6:00PM - 7:00PM E.B. Wilson Award Presentation
8:00PM - 9:30PM Exhibitor Tutorials
8:00PM - 10:30PM Film Session
8:00PM - 10:00PM NIH Peer Review


MONDAY, DECEMBER 11
7:30AM - 5:00PM Registration
7:30AM - 9:00PM Posters on Display
7:00AM - 10:00PM Exhibitor Showcase
8:00AM - 10:00PM ASCB/Carl Zeiss, Inc. Run
8:00AM - 9:30AM SYMPOSIUM III
Cell Adhesion in Differentiation
and Disease
Richard Hynes, MIT/HHMI, Integrin
and extracellular matrix function
in development
Mary Beckerle, University of Utah
(Chair), Cell adhesion-dependent
signalling
Judah Folkman, Harvard Medical
School, Endogenous inhibitors of
blood vessel growth
9:00AM - 4:00PM Exhibits open
9:00AM - 5:00PM Education/Minorities Affairs
Information Booth
9:30AM - 10:30AM Complimentary coffee and cookies in
Exhibit Hall
9:45AM - 10:15AM Education Coffee Break Forum
10:30AM - 12:00PM SYMPOSIUM IV
The Evolution of Eukaryotic Sex
Lawrence Hurst, University of
Cambridge, Selfish genetic elements
and their role in evolution: the
evolution of sex and some of what
that entails
Ursula Goodenough, Washington
University (Chair), Sex in simple
eukaryotes
Robin Lovell-Badge, Medical
Research Council, London, Sex
determination in mammals: the role
and evolution of the sry gene on
the Y chromosome
12:00PM - 2:00PM Women in Cell Biology Luncheon
12:00PM - 3:00PM Poster Presentations
1:00PM - 2:00PM NSF/Support for Improving Education
in Cell Biology
1:00PM - 2:30PM Graduate Student Symposium
3:00PM - 3:30PM Race awards at Zeiss booth
3:00PM - 3:30PM Refreshment break in Exhibit Hall
3:30PM - 5:45PM Minisymposia 7-12
5:30PM - 6:30PM ASCB Business Meeting
6:30PM - 7:30PM Women in Cell Biology Business
Meeting and Awards Presentation
7:30PM - 11:00PM Social, National Museum of Natural
History



TUESDAY, DECEMBER 12
7:00AM - 7:00PM Exhibitor Showcase
7:30AM - 5:00PM Registration
7:30AM - 9:00PM Posters on Display
8:00AM - 9:30AM SYMPOSIUM V
How Molecular Motors Work
Ron Vale, University of California,
San Francisco/HHMI (Chair),
Mechanical and structural studies
of the kinesin motor: are two heads
better than one?
Ivan Rayment, University of
Wisconsin, Structural basis of
myosin motility
Mary Porter, University of
Minnesota, Mutational analysis of
dynein regulation
9:00AM - 4:00PM Exhibits open
9:00AM - 5:00PM Education/Minorities Affairs
Information Booth
9:30AM - 10:30AM Complimentary coffee and cookies in
Exhibit Hall
9:45AM - 10:15AM Education Coffee Break Forum
10:30AM - 12:00PM SYMPOSIUM VI
Neuronal Connections: Establishment
and Plasticity
Marc Tessier-Lavigne, University of
California, San Francisco/HHMI
(Chair), Guidance of developing
axons to their targets
Story Landis, Case Western Reserve
University School of Medicine,
Interactions between pre- and
postsynaptic cells in the initial
establishment of synaptic junctions
Jeff Lichtman, Washington
University School of Medicine,
Cellular basis of activity-
dependent synaptic rearrangements
12:00PM - 3:00PM Poster Presentations
1:30PM - 3:00PM Practice of Science Panel
3:00PM - 3:30PM Refreshment break in Exhibit Hall
3:30PM - 5:45PM Minisymposia 13-18
7:30PM - 8:30PM Fourteenth Keith R. Porter Lecture
in Cell Biology
8:30PM - 10:00PM Exhibitor Tutorials



WEDNESDAY, DECEMBER 13
7:30AM - 3:30PM Registration
7:30AM - 3:00PM Posters on Display
8:00AM - 9:30AM SYMPOSIUM VII
Membrane Assembly from the Nucleus
to the Cell Surface
Randy Schekman, University of
California, Berkeley/HHMI (Chair),
Protein sorting during vesicle
budding
Douglass Forbes, University of
California, San Diego, Nuclear
assembly and transport
Richard Scheller, Stanford
University/HHMI, Mechanism and
regulation of membrane fusion
9:00AM - 3:00PM Exhibits open
9:00AM - 12:00PM Education/Minorities Affairs
Information Booth
9:30AM - 10:30AM Complimentary coffee and cookies in
Exhibit Hall
9:45AM - 10:15AM Education Coffee Break Forum
10:30AM - 12:00PM SYMPOSIUM VIII
Understanding and Controlling Cell
Biology through Synthetic Molecules
Roger Tsien, University of
California, San Diego/HHMI (Chair),
Dissection of a nitric oxide
signalling cascade using caged
compounds
Heidi Hamm, University of Illinois,
Chicago, Use of synthetic peptides
to investigate sites and mechanism
of G-protein action in signal
transduction
Stuart Schreiber, Harvard
University/HHMI, Chemical approach
to understanding and controlling
signal transduction
12:00PM - 3:00PM Poster Presentations and Special
Poster Session
1:00PM - 1:30PM Refreshment break in Exhibit Hall
1:00PM - 2:00PM NIH/A Walk on the Wild Side
3:30PM - 5:45PM Minisymposia 19-24
6:00PM Meeting ends



On Fri, 29 Sep 1995, Felicity Lawrence wrote:

} G'day.
}
} I'm looking for information regarding the American Society for Cell Biology.
} Could someone please tell me who to contact to get registration and
} accomodation details?
}
} Thanks,
} Felicity
} EM Unit, QUT. Brisbane, Australia
}
}




From: Owen Mills :      opmills-at-mtu.edu
Date: Sun, 1 Oct 1995 08:45:14 -0400
Subject: Re: Polaroid buckets

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Polaroid Type 55/665 negative racks and tanks can be purchased from

Graphic Center
P.O. Box 818
Ventura, CA 93002
(805) 383-6864

The PN-10 Clearing Tank includes a 4 quart plastic tank with lid and handle
and a 12 slot negative rack. It comes with 1 lb of sodium sulfite too.
Cost is $35.95 in August 1994 catalog.



At 09:45 AM 9/29/95 -0400, you wrote:
} Dear Bob: We have also spent an enormous amount of time searching for the
white buckets for the Polaroid 4"x5" negs. Polaroid
} informed us that they do not manufacture them. There are many Lucite racks
available for this purpose but if you work in the dark
} (like me) the racks are very difficult to use. It's like trying to thread
a needle in the dark. Should you find the white buckets please
} let me know. Thanks. Sincerely,
Charlie Murphy
}
}
Owen P. Mills
Michigan Technological University
Metallurgical & Materials Engineering
Rm 512 MME Building
Houghton, MI 49931
906-487-2002
906-487-2934 FAX
opmills-at-mtu.edu





From: MelanieOwl-at-aol.com
Date: Sun, 1 Oct 1995 22:06:42 -0400
Subject: Electrochemical AFM/STM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am looking for information about applications of electrochemical AFM or STM
cells. If anyone knows of any papers or has used electrochemical SPM for
their applications, I would appreciate your response.
Thanks, Melanie Behrens




From: Software department :      software-at-oimag.win-uk.net
Date: Fri, 29 Sep 1995 16:05:53
Subject: Re: Magnification Opinion

Contents Retrieved from Microscopy Listserver Archives
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X-Mailer: WinNET Mail, v2.30
Message-ID: {871-at-oimag.win-uk.net}
Reply-To: Software department {software-at-oimag.win-uk.net}
To: ZALUZEC-at-aaem.amc.anl.gov, Microscopy-at-aaem.amc.anl.gov
CC: ZALUZEC-at-aaem.amc.anl.gov

Yes, I would have said that myself having found magnification values
pretty meaningless when attempting to put a size on objects, but
this dialogue has made me realise why there is no totally correct
solution....

The micron marker concept only works if the depth of field is
negligible. That is the case with thin specimens in TEM, and almost
the case with flat specimens normal to the beam in SEM, and for
optical microscopes with very small depth of field but for rough or
inclined specimens in SEM or for photographs of large objects, the
size (micron) marker would have to be adjusted to match the depth of
the object behind the viewing port - not very easy to do unless the
object is viewed in true stereo.

Anyone got any tidy way round this problem of definition?

Peter Statham
Oxford Instruments Microanalysis Group
}
} Why bother with magnification at all. I always simply
} place a "micron" (or nanometer etc..) marker on the image.
} In this way the image is always calibrated regardless of
} what anyone else does to the print after you give it to them.
}
} Nestor
} Your Friendly Neighborhood SysOp
}

-----------------------------------------------------------------
Please reply to this e-mail with the name of the person you
wish to receive it on the subject line
(e.g. "FAO Janet Smythe/..subject.."),
as this is a shared e-mail address.







From: wesleysm-at-biology.und.ac.za (James Wesley-Smith)
Date: Mon, 2 Oct 1995 10:40:59 +0200
Subject: Re: background on tungsten film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Comments: Authenticated sender is {wesleysm-at-biology.und.ac.za}
"Simon Watkins Ph.D." {swatkins-at-pitt.edu}

Good day Simon
Your problem appears to be due to the colour temperature of the illumination not
matching that for which the film is balanced (3200K or 3400K) resulting in
reciprocity failure. This is why the fault is not seen on digital images.
Using daylight film and the microscope's blue filter, we consistently
got a reddish-brown background in our prints. (Using the recommended 9V setting
instead of 12V made matters 10x worse!) The answer to your problem lies
in establishing exactly what filters need to be inserted in the light path to
correct the colour temperature to suit the (tungsten) film. These will be in the 82
series, where each filter increases the temperature of the illumination by 100K.
Make sure to record whether the illumination diffuser is in or out
when you photograph using the different filters since it affects the temperature
of the illumination.
I would like to refer you to a Kodak publication, "Photography through the
microscope" by John Delly where you will find all the information you require.

In a perverse kind of way, I'm glad that we are not the only ones
with this kind of problem!

Hope this helps you.

James Wesley-Smith
Electron Microscope Unit
George Campbell Building
University of Natal
Durban, South Africa





From: Michael Cammer :      cammer-at-aecom.yu.edu
Date: Mon, 2 Oct 1995 11:34:24 -0400 (EDT)
Subject: Re: Resolution vs. magnification light microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have this problem constantly with users. They collect their images
with the BioRad confocal at different zoom settings or with Newvicon or
CCD video cameras and then ask for magnification. So I go into an
explanation of why magnification is not resolution. To have this
problem solved, I recommend they write in the figure legend what optics
and what electonic devices were used. I also recommedn adding scale
bars. The readers can immediately see the magnification and can also
judge the resolution if this is critical. Practically, this is a fair
trade off. If for phase contrast ot fluorescence we say we used a 100X N.A.
1.4 objective with t-Max 100 film or with the BioRad confocal, you have
an idea of the resolution. For critical applications, such as
microtubule motility assays, the methods would be more detailed. A
glance at the scale bar tells you magnification. Considering that
journals print with half-tone, this, practically, solves the problem.
Similarly, people zoom or shrink images on their computers without regard
for what is happening to the resolution of each pixel and the problem of
magnification vs. resolution is far worse than with film. -Michael C.




From: Richard E. Edelmann :      EDELMARE-at-CASMAIL.MUOHIO.EDU
Date: Mon, 2 Oct 1995 12:54:47 -500
Subject: Re: Polaroid buckets

Contents Retrieved from Microscopy Listserver Archives
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Yes, I too have used the lucite racks which due to the inherent
curl to the polaroid negs can be a real pain in the neck to use.
Additionally since the polaroid negs are thiner than normal
Kodak/Ilford/etc. type plate films, combined with their surface area
the polaroid negs then to pop out of the lucite rack slots very
easily. An alternative that I have found works very well is to use
Kodak Film Hanger No. 6, which are stainless steel and have two clips
which grab on to the edges of the film. These holders work for sheet
films from 3 inches wide to 6 inches wide with out difficulty, very
easily. The only problem is that the holders are expensive at $12.50
each! But most Kodak vendors can order these from Kodak and provide
them. The Film hangers will fit into any 4x5 neg developing tanks or
tupperware containers.






From: Eric Rosen :      earosen-at-indirect.com
Date: Mon, 2 Oct 1995 11:06:01 -0700
Subject: EM or lab tech positions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Microscopy group,

I know this is not allowed on the listserver but I am getting very
frustarted with job hunting

I am a grad student completing my M.A. in biology ac CSUF and am in
search or a EM technician position. I am listed with the MSA placement
office and have appled for every position they have had open and I have
sent out over 100 resumes. As of yet I have jeard from 8% of then all with
unfavorable results. I have found many postdoc positions that I can do but
they will not hrie a biologist with a M.A. I have looked in other cities
newspapers, on he internet and what ever other resource I can find.

I did not think it was this difficult to find a position. If anybody
knows of any fulltime permanent positions could you let me know Please?
If anyone would like to see a CV I can sent it to you

I have the following qualifications:

As part of my thesis work which is cellular and morphological, I am
investigating the morphological and biochemical characteristics of the
extracellular matrix of eggs. This work has revealed that the egg coats
swell when released into seawater and that this extracellular matrix is
composed of many types of sugar moieties , and ECM proteins as revealed by
lectin and antibody immunofluorescence labeling.

I have these and other qualifications:

Completed a course in Transmission Electron Microscopy with an
"A".

Completed lecture and lab course in Microbiology with "A" and "B"
respectively.

Utilized Electron Microscopy (TEM and SEM) as part of my Master's
Thesis research.

Knowledge of basic biochemistry skills (SDS PAGE, native gels,
westerns, and column chromatography).

Taught undergraduate laboratory class in mammalian physiology.

Immunolabeling techniques for light microscopy and electron
microscopy.

Proficient in Photomicrography (Light and EM level) and darkroom
skills.

Proficient with Word perfect, Quattro Pro, Sigmaplot, Sigmastat,
Freehand, Photoshop.

Proficient with computer vbased image analysis with a Compix
(c-imaging) image analysis system and CSPI (Scanalytics)
deconvolution software.

I have also learned the following techniques: immunolabeling (light
microscopy and EM), TEM, SEM, freeze-fracture TEM, sectioning
(ultramicrotome), EM technique of room temp. molecular shadowing ,
spectrofluorimetry, spectrophotometry, darkroom techniques and computer
based image analysis.

The results of the first part on my thesis research were published in
Molec. Biol.. Cell. 5, 94a. and Micros. Res. Tech., 29, (6) 495. (see
resume). These results were also presented at the American Society for
Cell Biology meeting in 1994, and the California State University EM
symposium in 1994.

I would appreciate an opportunity to discuss my academic and job
experience with you and to provide you with details not listed on the
enclosed resume. I am in the process writing my thesis. I can be reached
during the day at (602) 832-2885. I am highly motivated and look forward
to hearing from you soon.

Thank you,






Eric A. Rosen






From: Nina S Allen :      nallen-at-unity.ncsu.edu
Date: Mon, 2 Oct 1995 14:28:46 -0400 (EDT)
Subject: Re: Resolution vs. magnification light microscopy

Contents Retrieved from Microscopy Listserver Archives
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"Charles J. Butterick" {emccjb-at-ttuhsc.edu} ,
microscopy-at-aaem.amc.anl.gov

I routinely advise users of my electro optical equipment to record an
image of a stage micrometer along with their images. They also record
the settings of the microscope on the Universal Imaging set up . That is
keeping dual records. It is so much easier than back tracking.
Nina Allen.

On Mon, 2 Oct 1995, Michael Cammer wrote:

} We have this problem constantly with users. They collect their images
} with the BioRad confocal at different zoom settings or with Newvicon or
} CCD video cameras and then ask for magnification. So I go into an
} explanation of why magnification is not resolution. To have this
} problem solved, I recommend they write in the figure legend what optics
} and what electonic devices were used. I also recommedn adding scale
} bars. The readers can immediately see the magnification and can also
} judge the resolution if this is critical. Practically, this is a fair
} trade off. If for phase contrast ot fluorescence we say we used a 100X N.A.
} 1.4 objective with t-Max 100 film or with the BioRad confocal, you have
} an idea of the resolution. For critical applications, such as
} microtubule motility assays, the methods would be more detailed. A
} glance at the scale bar tells you magnification. Considering that
} journals print with half-tone, this, practically, solves the problem.
} Similarly, people zoom or shrink images on their computers without regard
} for what is happening to the resolution of each pixel and the problem of
} magnification vs. resolution is far worse than with film. -Michael C.
}




From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Mon, 02 Oct 1995 14:56:11 -0500
Subject: Re: Resolution vs. magnification light microscopy

Contents Retrieved from Microscopy Listserver Archives
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Message-Id: {199510021958.OAA21291-at-bcm.tmc.edu}
X-Sender: joiner-at-bcm.tmc.edu
X-Mailer: Windows Eudora Version 2.1.1
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One thing that you might try is to tell your people to make photographs of a
stage micrometer at the same equipment settings and to manipulate it the
same way they do their other micrographs. Then they would have a
magnification calibration in hand, that may be good only for the work they
did that day. But it would be a simple matter to calculate the actual
magnification of their finished work. After all the only mag value that is
important is the ratio of their final image, be it of their cells, tissue,
etc., and the specimen on their microscope stage, be it tissue or micrometer.



At 11:34 AM 10/2/95 -0400, you wrote:
} We have this problem constantly with users. They collect their images
} with the BioRad confocal at different zoom settings or with Newvicon or
} CCD video cameras and then ask for magnification. So I go into an
} explanation of why magnification is not resolution. To have this
} problem solved, I recommend they write in the figure legend what optics
} and what electonic devices were used. I also recommedn adding scale
} bars. The readers can immediately see the magnification and can also
} judge the resolution if this is critical. Practically, this is a fair
} trade off. If for phase contrast ot fluorescence we say we used a 100X N.A.
} 1.4 objective with t-Max 100 film or with the BioRad confocal, you have
} an idea of the resolution. For critical applications, such as
} microtubule motility assays, the methods would be more detailed. A
} glance at the scale bar tells you magnification. Considering that
} journals print with half-tone, this, practically, solves the problem.
} Similarly, people zoom or shrink images on their computers without regard
} for what is happening to the resolution of each pixel and the problem of
} magnification vs. resolution is far worse than with film. -Michael C.
}
}

Joiner Cartwright, Jr., Ph.D.
Director, Electron Microscopy
Department of Pathology, Rm.286-A
Baylor College of Medicine
One Baylor Plaza
Houston, Texas 77030 U.S.A.
tel.: (713)798-4658
FAX: (713)798-3945
joiner-at-bcm.tmc.edu
Compuserve: 71555,1206





From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Mon, 02 Oct 1995 14:42:53 -0500
Subject: Re: Magnification Opinion

Contents Retrieved from Microscopy Listserver Archives
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Message-Id: {199510021944.OAA20035-at-bcm.tmc.edu}
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Mime-Version: 1.0
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Probably the best solution is to add size-calibrated objects to the
specimen. Often SEM people will add latex beads of a calibrated size to
their samples. Of course this is not always practical. I often use naturally
occuring internal standards that show up in other peoples micrographs.
Collagen, microtubules, and other organelles of known size can be used in
this way, keeping in mind that specimen handeling can cause shrinkage. On
occasion I have caught errors in stated magnifications in this manner.






At 04:05 PM 9/29/95, you wrote:
} Yes, I would have said that myself having found magnification values
} pretty meaningless when attempting to put a size on objects, but
} this dialogue has made me realise why there is no totally correct
} solution....
}
} The micron marker concept only works if the depth of field is
} negligible. That is the case with thin specimens in TEM, and almost
} the case with flat specimens normal to the beam in SEM, and for
} optical microscopes with very small depth of field but for rough or
} inclined specimens in SEM or for photographs of large objects, the
} size (micron) marker would have to be adjusted to match the depth of
} the object behind the viewing port - not very easy to do unless the
} object is viewed in true stereo.
}
} Anyone got any tidy way round this problem of definition?
}
} Peter Statham
} Oxford Instruments Microanalysis Group
} }
} } Why bother with magnification at all. I always simply
} } place a "micron" (or nanometer etc..) marker on the image.
} } In this way the image is always calibrated regardless of
} } what anyone else does to the print after you give it to them.
} }
} } Nestor
} } Your Friendly Neighborhood SysOp
} }
}
} -----------------------------------------------------------------
} Please reply to this e-mail with the name of the person you
} wish to receive it on the subject line
} (e.g. "FAO Janet Smythe/..subject.."),
} as this is a shared e-mail address.
}
}
}
}
}

Joiner Cartwright, Jr., Ph.D.
Director, Electron Microscopy
Department of Pathology, Rm.286-A
Baylor College of Medicine
One Baylor Plaza
Houston, Texas 77030 U.S.A.
tel.: (713)798-4658
FAX: (713)798-3945
joiner-at-bcm.tmc.edu
Compuserve: 71555,1206





From: Eric Rosen :      earosen-at-indirect.com
Date: Mon, 2 Oct 1995 13:13:08 -0700
Subject: Coverletter

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To All:

I have a very good and folwing coverletter. To date I have had 7
professors and other faculty read it for me. They cannot se anything I
need to change with it. They all like how it flows and reads.


Eric A. Rosen






From: jbpawley-at-facstaff.wisc.edu (James Pawley)
Date: Mon, 2 Oct 1995 19:40:07 -0600
Subject: Macroscope vs. microscope

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} } } As a tangent to the current thread on magnification and resolution, our
} } } photographer today inquired about the difference between a macrograph
} } } (e.g., a photograph made using a macro lens) and a micrograph. Anyone
} } } want to jump in on this?
} } }
} } } James Martin
} } }
} } }
} } ************
} } OK, I'll give it a try. I am going to say that a camera with a macro
} } lens....IF it is set up to record images larger than the photographed
} } subject (ie. 1:1+).....is equivalent to a microscope with a film-back.
} } Therefore your photographer's "macrograph" is the same as a micrograph. I
} } think that macrograph may be a misnomer, or at the most, jargon. Many
} } photographers use macrography to mean recording images between what a
} } "standard" camera lens can do and what a close-up lens can do up to an image
} } ratio of 1:1, ie. not magnifying.

Macro photography is that which can be done conveniently with a single lens
and bellows.

Special macro-lenses are sold for this purpose and they work in the range
up to about 10x. This implies a lens-to-film distance about 10 times
lens-to-subject distance and, with a 25 mm lens, 10x about all you want to
try. In addition, such macrophotograpy setups are quite sensitive to
vibration and it is hard to arrange the lighting to provide an image bright
enough to focus the image on the ground glass. Finally, like all optics,
macro setups are limited by diffraction and aberrations and microscope
objectives can be better corrected because they only operate at a single
magnification. (while the macroscope magnification depends on the bellows
extention)

Jim Pawley

*****************************************
Prof. James B Pawley,
Ph. 608-263-3147
Room 1235, Engineering Research Building, FAX 608-265-5315
1500 Johnson Dr., Madison, WI, 53706 JBPAWLEY-at-FACSTAFF.WISC.EDU






From: Richard E. Edelmann :      EDELMARE-at-CASMAIL.MUOHIO.EDU
Date: Tue, 3 Oct 1995 09:58:27 -500
Subject: re: macrography vs micrography

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Whereas Joiner Cartwright stated that he feels macrography is a
misnomer and should not be used for anything over 1x I tend to
disagree though it maybe just a matter of semantics.

For the past 15 years I have been using the definitions provided by
Canon Inc.:
General Photography: 0.1x and lower
Close-up Photography: 0.1x - 1x
Photomacrography: 1x - 10x (- 20x)
Photomicrography: } 10x

I suppose these ranges are somewhat arbitrary but they do have
specific equipment required for each range:

General Photo.: normally-mounted standard lens alone.
Close-up: close-up lenses (macro lenses) or extension tubes
Macrography: main accessory is a bellows, often revese mounted
lenses (note: this is is acomplished with a single lens system
located at a distance from the film plane, there is no second lens
system.)
Micrography: replacment of 'normal' camera lenses with a
microscopic lens system, i.e. a compound light microscope. (Two
separated lens systems, the objectives and the projector/ocular)

{Granted I am not a optics specialist.}


Now this definition does present a small problem when dealing with
the differences between a compound light microscope [10 - 1,200x] and
a macroscope - a.k.a. Disecting scope (which is a really awful name!)
[4x - 250x]. All the journals I have dealt with insisted that the
term microscope be used and macroscope not be used, but it think that
this is in accurate as the two types of scopes are very different and
when I am reading a scientific publication I do like to know what
equipment is being used. The work depth of field at 100x in a
macroscope is much different than 100x in a microscope, eh?

But can we as a microscopy (including macroscopy?) community come to
some sort of a consensus on this?




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-aaem.amc.anl.gov
Date: Tue, 3 Oct 1995 9:26:46 -0500 (CDT)
Subject: Job Hunter/Posting

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G'day Colleague....

I know it sounds harsh, but Eric Rosen broke one
of our cardinal rules. I have sent him several notes
off-line and then deleted him from the subscription
list. I have said no to many people who have politely
asked about posting resume's some of which I know
personally. I have to keep a firm position here. No
offense meant to anyone looking for a job.

Nestor....

P.S.
I also pointed him to several places where he could
post and/or send resume's.




From: f.m.haug-at-basalmed.uio.no (Finn-Mogens Haug)
Date: Tue, 03 Oct 1995 15:33:35 +0200
Subject: Apology. Was: Administrivia.

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Dear list,

The following response to my recent query on bounced error messages,
requires an on-line answer.

}
} Dear Mr. Haug:
}
} Please be advised that the administrator of the Microscopy list server does
this on a voluntary, unpaid basis. Furthermore, the administrator, Dr.
Zaluzec, is a highly respected scientist, travels frequently, and often can
not respond on a timely basis to each request for help. Your incivility of
inferring that the administrator is inhuman is insensitivive and
unacceptable for this type of a forum. I also volunteer my time as an
administrator of a technical list server, and I can tell you from first hand
experience that it is a thankless task, a service that I render to
contribute to the sciences that I practice. I believe that you owe Dr.
Zaluzec, as well as the 2000 memebers of this list server, an apology.
}
} Respectfully submitted,
}

The paragraph which provoked this response was

} }
} } A few days ago, I reported this to Postmaster-at-aaem.amc.anl.gov,
} } hoping that she would be a human being, able to point out what is
} } wrong, which she is probably not since there has been no reply
} } in point.
} }

The above interpretation was !!not my intention. Sorry if clumsy writing
gave the impression of ingratitude towards Nestor and others who in their spare
time are building new communication channels for the scientific community.

I fully appreciate the amount of work it takes to raise and support a
large list, checking the message-flow, acquiring and implementing new
software versions, dealing with people responsible for the host machine,
budgetary issues, - - - .

Best regards,

Finn-Mogens

----------------------------------------------------------------------



Finn-Mogens Haug

University of Oslo
Institute of basic medical sciences
Department of anatomy E-mail: f.m.haug-at-basalmed.uio.no
Box 1105 Blindern Phone : +47 22 85 12 67
N-0317 Oslo, NORWAY Fax : +47 22 85 12 78






From: Alan S. Pooley :      pooley-at-ahab.rutgers.edu
Date: Tue, 3 Oct 1995 10:36:40 -0400 (EDT)
Subject: Re: Polaroid buckets

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I have made and used for 20 years lucite holders for polaroid film.
I have 2 holders 18.5 *11.3 * 11 cm high with handles 18 cm high
with 11 slots 1.3 * 10 * 11 cm high. These slots are designed to
hold the film emulsion side concave inward so the solution acts on the
emulsion and it does not curl the other way. In practice, they still
curl back but it does not hurt. I soak in water 1 minute to 8 hours
(ie at least a short soak but all day is ok) then 18 % sodium sulfite
as advised by polaroid then 5 minutes (to several hours but not over
night) in running tap water, rinse in Deionized water and transfer to
second racks (below). Occasionally, as the sulfite gets old I have to
squeegee off a little jelly clinging to the surface. These racks
are designed to fit standard 1 gallon stainless steel tanks.
The second rack, which I have 8 but could sometimes use 12 or more,
are copied after some commercial racks with curling slots but my slots
are further apart (the commercials ones went out of business 15 years ago).
These drying racks are 23 * 11.5 * 15.5 cm high and have 10 curving slots
down the side of the long axis to hold 10 films while drying. 11.5 cm
below the top is a lucite bar with straight slots to hold the bottom
of each film. The curving slots are spaced 2 cm apart, are 12.5 cm high,
0.3 cm deep and 0.2 cm wide. The curve is bell shaped, flat at top and
bottom and the curve is displaced 1.2 cm 'sideways' in the middle
( about 8 cm of the slot is humped sideways. These slots are formed from
0.2 cm thick lucite, cut with the curve both sides and glued (acetone) to
the side plates of the lucite rack. Some care is needed to avoid curling
of the film or the negative become stuck together ( a long soak in hot
water will enable almost all such stuck negatives to be rescued, but
prevention is easier. These racks do not fit a tank and are never used
for soaking, only drying.
I would be willing to post details, if enough people are interested.

Sorry about the length of this.

Alan S. Pooley ,PhD Bivalve shell SEM & shape analysis
SEM/Morphometrics lab
Marine & Coastal Sciences
Rutgers University
908 932 8959 ext 225
Pooley-at-ahab.rutgers.edu

On Mon, 2 Oct 1995, Richard E. Edelmann wrote:

} Yes, I too have used the lucite racks which due to the inherent
} curl to the polaroid negs can be a real pain in the neck to use.
} Additionally since the polaroid negs are thiner than normal
} Kodak/Ilford/etc. type plate films, combined with their surface area
} the polaroid negs then to pop out of the lucite rack slots very
} easily. An alternative that I have found works very well is to use
} Kodak Film Hanger No. 6, which are stainless steel and have two clips
} which grab on to the edges of the film. These holders work for sheet
} films from 3 inches wide to 6 inches wide with out difficulty, very
} easily. The only problem is that the holders are expensive at $12.50
} each! But most Kodak vendors can order these from Kodak and provide
} them. The Film hangers will fit into any 4x5 neg developing tanks or
} tupperware containers.
}
}
}




From: George.C.Ruben-at-Dartmouth.EDU (George C. Ruben)
Date: 03 Oct 95 07:38:09 EDT
Subject: Re: Magnification Opinion

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Message-id: {4731381-at-prancer.Dartmouth.EDU}


Usually images taken with the TEM are focused on a specimen's high point at
~0-100A underfocus so that the specimen beneath is underfocused. In a high
resolution instrument with a focal length of 2.0 mm Scherzer focus occurs at
450-750A underfocus and the useful depth of field is not much larger than this
value. In an ordinary TEM with a focal length of 5 mm, Schertzer focus occurs
at about 1350-1400 A with a useable depth of field also extending to a larger
value. If you calculate depth of field for the 5 mm focal length instrument, it
is ~2650A however about a third of this depth may be too underfocused to be
useful. My question is how much does the magnification change for these
underfocus values? My impression is that freeze dried objects of known size,
50-250A, are approximately the same size over this range of focus values and
the change in magnification is small! This observation suggests that a
magnification bar on a TEM micrograph is a good estimate of the size of objects
in an enlarged micrograph!

George C. Ruben
Dept. Biological Sciences
Dartmouth College
Hanover, NH 03755




From: mmundsc-at-bgnet.bgsu.edu (Michael Mundschau)
Date: Tue, 3 Oct 1995 13:52:45 -0400
Subject: Conference Toledo Oct 12-14, 1995

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It is not too late to register for the Great Lakes Microscopy Conference to
be held at the Toledo Hilton Dana Center in Toledo, Ohio, October 12-14.
Talks are on various areas of microscopy-Biology, Materials Science,
Pathology, Forensic Science etc. Student Registration $20.00 Non-student
$30.00. Contact C.Heckman at 419-372-8218 e-mail: heckman-at-bgnet.bgsu.edu
for further details.







From: VETO-at-BCRSSU.AGR.CA
Date: 03 Oct 1995 14:13:56 -0400 (EDT)
Subject: Macro... vs. micro...

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"...inquired about the difference between a macrograph (e.g., a photograph
made using a macro lens) and a micrograph."

macro-: in Greek comp., long, large, thick, great;
micro-: in Greek comp., little, small, minute;

Stereo-microscopes, hand held glass lenses, any macro-setup and SEMs are
used to increase the surface morphology of the specimen by reflected light
. SEMs form SE or X-ray image of the "bulk" sample's surface, regardless
it's fractured or not. These are macro images.

When the image is formed by using trasmitted light or e-beam, the "micro"
internal image of the section is formed. (LM, TEM...) Sections are produced
by "micro"tomes or ultra"micro"tomes to expose the internal fine stuctures
of the specimen. These are micro images.

I hope it helps.

Laszlo J. Veto Tel: 604-494-7711
Electron Microscopist Fax: 604-494-0755
Research Centre e-Mail Veto-at-bcrssu.agr.ca
Summerland, B.C.
Canada V0H 1Z0




From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Tue, 03 Oct 1995 14:50:50 -0500
Subject: LKB service

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Does anybody know who services LKB ultramicrotomes in the Houston area? I
just inherited an LKB Nova and it needs some attention.

Thanks



Joiner Cartwright, Jr., Ph.D.
Director, Electron Microscopy
Department of Pathology, Rm.286-A
Baylor College of Medicine
One Baylor Plaza
Houston, Texas 77030 U.S.A.
tel.: (713)798-4658
FAX: (713)798-3945
joiner-at-bcm.tmc.edu
Compuserve: 71555,1206





From: EMLAB-at-opus.mco.edu
Date: Tue, 03 Oct 1995 16:21:36 -0500 (EST)
Subject: Re: EM or lab tech positions

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Hi Eric,
Just to let you know, it took me almost 2 years to find a new job.
Hang in there and they will come. The best way was to knock on doors, very
few technicial postions are advertised outside the institution. A face to face
meeting is much better than any CV.

Best of Luck,
Ed Calomeni
Dept Pathology
Medical College of Ohio
Toledo, OH 43699
emlab-at-opus.mco.edu




From: bell-at-medcolpa.edu (Ted Bell)
Date: Tue, 03 Oct 1995 17:38:24 -0400 (EDT)
Subject: sectioning =?iso-8859-1?Q?500=B5m?= sections

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I am having problems sectioning tissue blocks that are 500=B5m thick, I woul=
d
like to have 10; 50=B5m sections in the end. Using a vibratome I have tried
mounting the tissue onto 15% gelatin, the tissue seems secure but then
falls off the chuck before I'm finished cutting. I can't use superglue, it
messes up the tissue more than I do. I need to use the vibratome - this is
essential. I tried using the cryostat and have gotten good sections,
however I was not able to stain the sections. Can anyone give me
suggestions on how to secure a 500=B5m slice onto a chuck so that the tissue
in fairly flat and won't fall off?


Ted Bell, bell-at-medcolpa.edu
Medical College of Pennsylvania and Hahnemann University
Neurobiology and Anatomy
http://ussanatomy.medcolpa.edu/FaberWeb/confocal.html






From: scasson-at-metz.une.edu.au (Shane Casson)
Date: Tue, 3 Oct 1995 22:21:59 -0700
Subject: Unsubscribe

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Unsubscribe please?






From: Carl Henderson :      chender-at-umich.edu
Date: Tue, 3 Oct 1995 20:28:25 -0400 (EDT)
Subject: Re: Macro... vs. micro...

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I always thought "macro"-whatever referred to an object which was visible
to the unaided eye, whereas "micro"-whatever was discernible only with the
aid of some kind of magnifying device, such as a microscope.

Certainly objects can be discernible, yet still small, and require
special setups for photography. Hence, macro lenses.

Carl

Carl Henderson
Electron Microbeam Analysis Laboratory
University of Michigan
2005 C.C. Little Bldg.
Ann Arbor, MI 48109-1063 USA
--------------------------------
(313) 936-1550 (voice)
(313) 763-4690 (FAX)
chender-at-umich.edu (e-mail)
--------------------------------




From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Wed, 4 Oct 1995 12:52:02 GMT+1200
Subject: XRF/XRS listserver

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Does anybody know of a listserver/group similar to this one but which
is concerned with X-Ray Spectrometry (XRS) or X-Ray Fluorescence
(XRF)?


Ritchie Sims phone: 61 9 3737599 ext 7713
Department of Geology fax: 61 9 3737435
University of Auckland
Private Bag 92019
Auckland
New Zealand




From: pathgs-at-wnmeds.ac.nz (Gracie Solomon)
Date: Wed, 4 Oct 1995 12:52:02 GMT+1200
Subject: Unsubscribe

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From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Wed, 4 Oct 1995 14:29:47 GMT+1200
Subject: SPI Mineral standards for quantitative analysis

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Tiring of the struggle to maintain and build up a suitable collection
of mineral standards for the calibration of the elderly electron
microprobe which I run/maintain/love/hate, I am near to the point of
ordering from SPI their 53-mineral mount cat # 02753-AB.

Has anyone out there used this product for standardisation for
quantitative geological analytical work? Found it to be good? Should
I buy it?

Maybe email me direct to avoid commercial sensitivities, although I
don't think that Charles Garber would shy from open debate, would
you, Charles?

thanks

Ritchie Sims phone: 61 9 3737599 ext 7713
Department of Geology fax: 61 9 3737435
University of Auckland
Private Bag 92019
Auckland
New Zealand




From: Stefan.Gunnarsson-at-devbiol.uu.se (Stefan Gunnarsson)
Date: Wed, 4 Oct 1995 09:30:44 +0100
Subject: re: macrography vs micrography

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} Whereas Joiner Cartwright stated that he feels macrography is a
} misnomer and should not be used for anything over 1x I tend to
} disagree though it maybe just a matter of semantics.
}
} For the past 15 years I have been using the definitions provided by
} Canon Inc.:
} General Photography: 0.1x and lower
} Close-up Photography: 0.1x - 1x
} Photomacrography: 1x - 10x (- 20x)
} Photomicrography: } 10x
}
} I suppose these ranges are somewhat arbitrary but they do have
} specific equipment required for each range:
}
} General Photo.: normally-mounted standard lens alone.
} Close-up: close-up lenses (macro lenses) or extension tubes
} Macrography: main accessory is a bellows, often revese mounted
} lenses (note: this is is acomplished with a single lens system
} located at a distance from the film plane, there is no second lens
} system.)
} Micrography: replacment of 'normal' camera lenses with a
} microscopic lens system, i.e. a compound light microscope. (Two
} separated lens systems, the objectives and the projector/ocular)
}
} {Granted I am not a optics specialist.}
}
}
} Now this definition does present a small problem when dealing with
} the differences between a compound light microscope [10 - 1,200x] and
} a macroscope - a.k.a. Disecting scope (which is a really awful name!)
} [4x - 250x]. All the journals I have dealt with insisted that the
} term microscope be used and macroscope not be used, but it think that
} this is in accurate as the two types of scopes are very different and
} when I am reading a scientific publication I do like to know what
} equipment is being used. The work depth of field at 100x in a
} macroscope is much different than 100x in a microscope, eh?
}
} But can we as a microscopy (including macroscopy?) community come to
} some sort of a consensus on this?

Yes, it would indeed be good if microscopists could agree on some sort of
consesus on the terminology. My opinion on the matter is that the Canon
definitions can well be used as a standard (even though the distinction
between macro- and micrography creates some difficulties with different
microscope systems, see below). The only question is how can "we" get
everyone (more or less) to stick to a standard whether it is this one or
something else that "we" can agree on?!

(This is actually quite an interesting problem in itself: can a discussion
group like this one come to a consesus on a certain matter, and how should
that then be implemented? In a conference or society meeting with chairman
etc. there can be taken a "democratic" decision, but how do we do here?)

Back to microscopes and the distinction between different types: A compound
microscope consists of two sets of optics (objective and ocular) that form
the image and illumination which can be incident, transmitted, or sideways.
It doesn't really matter wether you are looking at the surface of a
specimen or through a section of the specimen. (Leitz used to make an
excellent set of optics called the "Ultropak" system with lenses up to 40x
that could be immersed in water solutions and used for very close up
studies of dissected animals. You can find good example of that on the
cover of the May 1995 issue of J. Exp. Biol. It is a pity that no company
(at least to my knowledge) makes anything like that anymore.)
The main difference between microscopes in the imaging process for the
human eyes is if there are one or two objective lenses. With one lens you
get a "normal" LM, with two lenses you get a stereo microscope which needs
objectives with long working distance (i.e. long focal distance and low
N.A.) to make possible and meaningful to use stereo imaging. Of course if
you attach a camera to a stereo micrscope you only use one of the
objectives and consequently loose the stereo effect (which in any case is
pointless since the camera is one-eyed).

Maybe this hasn't clarified things a lot, but at least I hope that "stereo
microscope" might be used more rather than "dissecting scope" or the
completely wrong Swedish word "lupp" which too many of my compatriots use
(it basically just means magnifying glass!)

Thanks for your attention!

Stefan


..............................................................................

Stefan Gunnarsson
Microscopy Unit,Dept. of Animal Development and Genetics

Uppsala University
Norbyv. 18A, S-75236 UPPSALA, Sweden
e-mail Stefan.Gunnarsson-at-devbiol.uu.se








From: HALTERSA-at-ctrvax.Vanderbilt.Edu
Date: Wed, 04 Oct 1995 07:43:17 -0500 (CDT)
Subject:

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set microscopy mail postpone




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-aaem.amc.anl.gov
Date: Wed, 4 Oct 1995 7:29:32 -0500 (CDT)
Subject: DNS Errors

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Hi All


The main DNS server at ANL went on the blink a few days ago.
Warning. You may see a rash of undeliverable mail. Hold off
posting messages for a day or so until you see a new posting
from me saying that everything has been corrected....

OR post at your own risk and be prepared for undeliverables.

Nestor





From: hawkey-at-neuro.duke.edu (Larry Hawkey)
Date: Wed, 4 Oct 1995 09:33:59 -0500
Subject: Jeol 1200exII forsale

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For Sale JEOL 1200exII Conventional TEM. (straight TEM with no add-
ons) This instrument is less than five years old and has been under service
contract the entire time and is in mint condition. It has had limited users
and all users were closely supervised by me; as a result it has had no down
time.
We are currently taking bids.
send bids to:
Susan Havrilesky
Box 3209 DUMC
Duke University Dept of Neurobiology
Durham NC 27710

For more information call (919) 681-6425 or E-Mail me (Larry Hawkey)
hawkey-at-neuro.duke.edu.






From: USERHHXS-at-um.cc.umich.edu[SMTP:USERHHXS-at-um.cc.umich.edu]
Date: Wed, 4 Oct 1995 22:28:39 +-900
Subject: UTW vs Be Windows

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Message-ID: {01BA92A8.C3D22480-at-ppp229.gol.com}



----------

I'm interested in user's experience regarding the relative=20
performance of UTW vs Be window EDS detectors for the
detection of high Z materials (i.e. rare earths). My own
personal experience tends to favor the latter, although
I can't, off hand, think of an inherent reason why this=20
should be so.

One reason that you might see better performance when acquiring spectra =
from high-Z elements with a beryllium window, as compared to an =
ultra-thin window, is that the detector with beryllium window may be =
doing a better job of preventing back-scattered electrons from entering =
the spectrum. =20

You can determine whether back-scattered electrons are the culprit by =
examining the shape of the brehmstrahlung at the higher energy region. =
Back-scattered electrons entering the detector will typically cause a =
smooth "hump" in the spectrum above about 20keV.

If you find that this is the problem, you can try changing the =
detector/sample geometry slightly, or check with the EDS manufacturer to =
find out if an improved electron trap is available. =20

- Jeff Allbright







From: GVKM07A-at-prodigy.com (DR CHARLES A GARBER)
Date: Wed, 04 Oct 1995 09:28:40 EDT
Subject: SPI Mineral standards for quantitative analysis

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-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --

On October 3, Ritchie Sims asked about the SPI #02753-AB Mineral
Standards and further asked if I would mind an open debate on this
subject!

Of course I wound not mind! And if anyone has ideas on how we can make
what we think is an outstanding product a bit better, I would thank you
in advance for any such suggestions, publicly or privately.

Homogeneity of course is everything when it comes to mineral standards,
and the individual mineral grains used in the mounts are selected with
that particular thought in mind. The product is also very easy to use
because of the built in Faraday cage and also the electron beam
lettering opposite each mineral giving its identification, which can be
"read" in the SEM or microprobe (but with mirror image lettering).

Chuck

======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail:
GVKM07A-at-prodigy.com
West Chester, PA 19381-0656 USA Customer Service:
SpiSupp-at-aol.com

########################################################
WWW: http://mail.cccbi.chester.pa.us/spi/spihome.html
########################################################
======================================================





From: dietmar.reiter-at-uibk.ac.at (Dietmar Reiter)
Date: Wed, 4 Oct 1995 16:06:51 +0100
Subject: Optical simulation applications

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Dear Colleagues,

I=B4m looking for *graphical* simulation programs for Optics (Macintosh
preferably, but others are welcome). I=B4m thinking of an application, which
can be used like an optical bench used in physics (Optical axis where
lenses, lasers, diaphragms, and else can be assembled). I would like to
demonstrate the effects of:

- basic optical laws, refraction of light rays through lenses
- diffraction effects and image formation (in microscopes)
- diffraction patterns (light -} grid)
- lens abberations (spherical, chromatic, distortions)
- effects of depth of focus, etc.

in basic microscopy courses. Anything like that available?
Thanks for any help, sincerely -Dietmar-


+++ Dietmar Reiter ++++++++++++++++++++++++++++++++++++++++++++
+++ Dept. of Zoology and Limnology office 1: (+43)-512-507-6170
+++ University of Innsbruck office 2: (+43)-512-507-6161
+++ Technikerstrasse 25 fax1: (+43)-512-507-2930
+++ A - 6020 Innsbruck, Austria fax2: (+43)-512-507-2957

... If I would have had more time,
I would have written a shorter e-mail ...






From: Mount, Richard :      MOUNTR-at-hermes.is.sickkids.on.ca
Date: Wed, 04 Oct 1995 08:17:00 -0500
Subject: macro vs micro

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Message-Id: {Megw.147982-at-hermes}


{Text_1}
I believe that the main difference between photomacrography
and photomicrography is related to the optics used in
obtaining the image. A micrograph is taken using a compound
microscope (i.e. one where the image formed by the objective
lens is further magnified by an eyepiece lens) whereas a
macrograph is taken using a simple magnifier (i.e. single
lens). At equal magnifications a macrograph will have
greater depth of focus but lower resolution than a
micrograph.
Other differences relate to methodology. A
photomacrographer usually approaches a subject and lights it
according to photographic conventions (main light, fill, key
lights etc.) using smaller than usual equipment such as
dental mirrors, bits of foil, and small cards. The
photomicrographer usually brings the specimen to the
microscope and lights it as one would for routine
observation (bright or dark field, epi, phase etc.).


Richard Mount

************************************************************
Richard J. Mount Phone: 416-813-6551
Auditory Science Laboratory FAX: 416-813-5036
Department of Otolaryngology
The Hospital for Sick Children
555 University Avenue
Toronto, ON, Canada M5G 1X8

e-mail: richard.mount-at-mailhub.sickkids.on.ca




From: James W. Adams :      jwa2n-at-avery.med.virginia.edu
Date: Mon, 25 Sep 1995 03:54:47 +0000
Subject: Standard slides for fluor.

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Message-Id: {Chameleon.951004105357.tonygr-at-emlab.mit.edu}

On the Sep 22nd, I asked for sources of " 'non-bleaching', inorganic, specimens
suitable for 1/ routinely checking excitation level and for 2/ correcting
spatial uniformities of the optical/digital imaging system. - ". The query
was posted to sci.techniques.microscopy, CONFOCAL-at-UBVM.CC.BUFFALO.EDU and
microscopy-at-aaem.amc.anl.gov, and the summary is similarly posted as more than
20 persons asked for it. It runs 5 pages.

Some answers dealing with fluorescent beads are omitted. For 1/ and 2/ above,
solutions made to desired specifications were recommended as flexible and
traceable homogeneous standards, which do not bleach if a sufficiently large
chamber is used, allowing fresh fluid to diffuse in under the beam.

Solid plastics of suitable fluorescence for flat-fielding is obtained from
commodity stores; the exact sources were not specified, nor product name,
composition or spectrum. None of the answers dealt with home-made
fluorescent plastics, i.e. from epon or lowicryl.

Uranyl-glass is no longer produced, for environmental reasons, but available
from distributors. Its radioactivity was not quantified, and probably
assumed to be
of no concern when the glass is used as microscope slides [should be
tested?] Other
inorganic standards than uranyl glass were not suggested. Here are the original
answers, slightly edited.

*********************************** Solutions
*************************************




**************************** Solid plastics ********************************






********************** Uranyl and other inorganic standards ****
several messages refer to
Newport Industrial Glass, Inc
1631 Monvrovia Avenue, Costa Mesa, CA 92627, Tel 714-642-9980, Fax
714-645-6800,
Bill Larsen. I phoned them and was transferred to Ray Larsen (brother) who
immediately faxed an offer for microscope-slide sized glass plates, with
curves for percent transmittance at different wavelengths (no fluorescence
spectra. There are two qualities of glass (#3750 with refractory index,
n= 1.509 and #3780 with refractory index, n= 1.533).

Date: Mon, 6 Feb 1995 09:52:54 -0800
From: George M {George_M-at-image1.com
Uranyl glass slides - - - from Newport Industrial Glass Inc.[ -
- -- - ]
Sold as a 6.5x6.5" sheet (you can specify 1 mm height), so you may want
to form
a "consortium" to have Newport pre-cut a sheet to slide-size. - - - If
there
is a lotof interest, my company may start selling single slides.
Dr. George McNamara
Universal imaging corporation

Date: Wed, 5 Jan 1994 09:27:20 -0500
From: "Bill Bug (Bill bug)" {bbug1-at-CC.SWARTHMORE.EDU
You can obtain micro slide sized pieces of uranyl glass from:
Newport Industrial Glass Inc [- - - - - - - - -] They have this glass
(glass
#3750) in large stock pieces, so it must be cut down to the size of a micro
slide. They will grind it down to whatever thickness you desire as well. We
ordered two micro slide sized pieces in July of 1993. The cost was $86.
Bill Bug, Department of Biology, Swarthmore College






[** Added by FMH:
From Schott Glasswerke, Postfach 2480 D-55014 Mainz, Germany,
Tel +49 61 31 660, Fax +49 61 31 66 20 00,
we were referred to Schott Glass Technologies, Inc, 400 York Avenue, Dureya,
PA 18642, Tel +1 717 457 7485, Fax +1 717 457 6960, Ref. Mr Steve Sokach. The
latter company makes Rare Earth Doped Filter Glass with specified absorption
spectra, but so far no data on fluorescence has been received. A fax to the
latter number brought no answer so far.**]
Finn-Mogens Haug

University of Oslo
Institute of basic medical sciences
Department of anatomy E-mail: f.m.haug-at-basalmed.uio.no
Box 1105 Blindern Phone : +47 22 85 12 67
N-0317 Oslo, NORWAY Fax : +47 22 85 12 78






From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-aaem.amc.anl.gov
Date: Wed, 4 Oct 1995 12:18:08 -0500 (CDT)
Subject: BITNET Email from Microscopy

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All Bitnet Subscribers to Microscopy.....

I regret to inform you that the ANL (Argonne National Lab) link
to BITNET is being terminated. The Microscopy Server will nolonger
be able to forward mail via BITNET. If you are recieving mail
via this route you will have to change your service provider
and resubscribe with your new address.

Sorry, but I have no control over this one...

Nestor
Your Friendly Neighborhood SysOp.




From: Michael OKeefe :      Michael_OKeefe-at-macmail7.lbl.gov
Date: 4 Oct 1995 11:54:58 U
Subject: Subscribe

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Message-Id: {n1399314565.86909-at-macmail7.lbl.gov}

Subject: Time:12:01 PM
OFFICE MEMO Subscribe Date:10/4/95

Please re-subscribe maok-at-lbl.gov





From: NICK SCHRYVERS :      nick_schryvers-at-ruca.ua.ac.be
Date: 5 Oct 1995 10:02:05 +0100
Subject: Re: UTW vs Be Windows

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Message-Id: {n1399234564.73900-at-ematserv.ruca.ua.ac.be}

Reply to: RE} UTW vs Be Windows

Just a technical, maybe obvious note coming from experience: the thinner the
window, the easier it fails as a vacuum seal which means several weeks of
repair and inconvenience. Take this into consideration when choosing.
Nick Schryvers







From: mecavaleri-at-mmm.com
Date: Thu, 5 Oct 1995 07:32:36 -0500
Subject: Macrograpghy Micrography

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I have been following the comments about macrography and micrography with
much interest. Of particular interest to me are the comments concerning some
sort of standardization of usage. ASTM does have at least one standard
(E7-95 Standard Terminology Relating to Metallography) which could be used

MACROGRAPH - a graphic reproduction of an object, slightly reduced in size,
unmagnified, or magnified ten diameters or less (photomacrograph).

MICROGRAPH - a graphic reproduction of an object as seen through the microscope
or equivalent instrument, at magnifications greater than ten diameters
(photomicrograph).

While these definitions are not perfect - they are contained in an accepted
standard - thus they may be of use. Note there are also some older ASTM
standards which are no longer in service that contain definitions relating to
micrography and macrography.

The New York Microscopical Society has published a GLOSSARY OF MICROSCOPICAL
TERMS AND DEFINITIONS which contains a set of less consistent definitions.

Are there any MSA documents which could be used to standardize the usage of
such terms ?

Mark E. Cavaleri
3M CRL/A&PRL Light Microscopy
3M Center 201-1E-15
St. Paul, MN 55144-1000
(612) 733-3247
(612) 733-0648 FAX
mecavaleri-at-mmm.com





From: wesaia-at-iastate.edu (Warren Straszheim)
Date: Thu, 05 Oct 1995 08:23:14 -0500
Subject: RE: UTW vs Be Windows

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} There are a number of issues relating to the choice between UTW and
} Be windows. Some of these are:
} ...
} Count rates: The light elements can contribute a significant number of
} counts to the spectrum, especially in a TEM. These can increase the
} throughput of the pulse processor, degrading the resolution and increasing
} the dead time. Since the low-energy counts have to be recorded, the pulse
} discriminators must be set down into the noise, further increasing the number
} of counts that must be processed. Hence, depending on the samples, the
} number of counts recorded from the heavier elements will be less for a UTW
} detector running at maximum count rate than for a Be-window detector in
} the same situation.
} ...
} Tony.
} *********************************************************
} * Anthony J. Garratt-Reed *
} * Massachusetts Institute of Technology, Rm. 13-1027 *
} * Cambridge, MA 02139, USA *
} *********************************************************

I am curious obout your statements on count rate. Certainly the Be window would
attenuate the low energy pulses including the light elements and low-end
noise so that the pulse processor would not have to process them at all.
You said that the
low-energy counts have to be recorded with a UTW detector. What about just
turning
up the fast discrminator to throw away the light element x-rays in addition
to the
noise? How much of a burden would that put on the pulse processor as opposed
to the
case with a Be window which absorbs those x-rays?
----------------------------------------------------
Warren E. Straszheim, Ph.D.
270 MD Ames Lab/ISU, Ames IA, 50011
Phone: 515-294-8187
FAX: 515-294-3091
E-Mail: wes-at-ameslab.gov (or: wesaia-at-iastate.edu)

coal characterization and processing
electron microscopy, x-ray analysis, image analysis
computer applications





From: Giles John E Jr :      giles_john_e_jr-at-space.honeywell.com
Date: 5 Oct 1995 09:56:47 U
Subject: Laser Printers

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Good Morning:

I am looking for input from list members on high resolution laser printers for
printing digital images from our Auger systems. We have the ability to capture
to a PC, and would like to print out these images. The quality of the image
system is not real good, so the laser will probably provide sufficient
resolution. The Auger system software also limits what we can use. The images
must be printed off the print command from the PC since the software contains
the mag and title data (not part of the image file). If it was a part of the
file we could send data to our SEM PC which is connected to a Codonics VP4500
thermal printer.

Any information/experiences from the list members on laser printers would be
greatly appreciated. You can reply to the list or directly to me.

Thanks,

John Giles (jegiles-at-space.honeywell.com)
Honeywell Space Systems
Clearwater, FL




From: _ _ _ _ _ MARK W. LUND _ _ _ _ _ :      LUNDM-at-PHYSC2.BYU.EDU
Date: Thu, 5 Oct 1995 08:19 MDT
Subject: RE:UTW vs Be Windows

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Nick Schryvers has pointed out that ultra thin windows fail easier than
beryllium windows. While this is true to some extent, ultra-thin
windows are extremely reliable. The last time I compiled data in
this area was around May 1993 for my article on UTW's in "X-ray
spectroscopy in electron-beam instruments." At that time mean time
before failure was seven and a half years and rising. (rising because
we have only had windows in the field since about 1990). Many of
the field failures were due to causes that would also have broken
a Be window. Some microscopes are harder on UTWs than others,due
to the specific flow of turbulance around the window during venting,
but these problems have been identified and fixes are available.

Tony is right that a Si(Li) detector can only handle one event at a
time, whether inside or outside of the region of interest. Opening
the detector to soft x-rays will increase the counts processed whether
discriminated out by the electronics or not.

best regards
mark

Mark W. Lund, PhD
Director
MOXTEK, Inc.
Orem UT




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-aaem.amc.anl.gov
Date: Thu, 5 Oct 1995 22:23:22 -0500 (CDT)
Subject: THIS IS A TEST DONOT REPLY...Nestor

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For those of you that read this I have shut down the server
to reconfigure the DNS service. I've waited for the queues
to clear and will see how many Undeliverables I get with this
message. If it's only a few then the problem is cured. If not
then it will be another day or so...

The problem arose due to a change in the DNS service here at the
lab and the software that ANL uses to move mail from their
various computers out to the net. I use Multinet on this computer
and Multinet has had to be reconfigure to match the local and
net wide changes....

Nestor




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-aaem.amc.anl.gov
Date: Tue, 10 Oct 1995 7:41:35 -0500 (CDT)
Subject: Sorry Folks, I need one more test message- Nestor

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Sorry Subscribers,

I've made a fairly massive change last night.
I need yet another test message, I hope it's the
last. I think everything is okay and we will be
back on-line shortly. With a list this size
I do not want to start another round of bouncing mail.

Bear with me....

Nestor

Your Friendly Neighborhood SysOp




From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Tue, 10 Oct 1995 11:06:14 +0200
Subject: Removing Dow Corning Silicone HV Grease from TEM sample holder

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From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Tue, 10 Oct 1995 11:06:14 +0200
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-----------------------------------------------------------
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From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Tue, 10 Oct 1995 11:05:45 +0200
Subject: EDS & STEM - Concentration profile

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From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Tue, 10 Oct 1995 11:05:45 +0200
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From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Tue, 10 Oct 1995 12:39:19 +0200
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From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Tue, 10 Oct 1995 12:39:19 +0200
Subject: SCANNING 96

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From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Tue, 10 Oct 1995 12:42:01 +0200
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From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Tue, 10 Oct 1995 12:42:01 +0200
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-----------------------------------------------------------






From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Tue, 10 Oct 1995 12:38:43 +0200
Subject: IFSEM Secretariat

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From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Tue, 10 Oct 1995 12:38:43 +0200
Subject: IFSEM Secretariat

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From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Tue, 10 Oct 1995 12:41:24 +0200
Subject: Wanted: Used TEM

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From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Tue, 10 Oct 1995 12:41:24 +0200
Subject: Wanted: Used TEM

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From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Tue, 10 Oct 1995 11:05:59 +0200
Subject: Microscopy Meeting (AReMS), Kingsport, TN

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From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Tue, 10 Oct 1995 11:05:59 +0200
Subject: Microscopy Meeting (AReMS), Kingsport, TN

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From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Tue, 10 Oct 1995 12:39:36 +0200
Subject: SEM of Daphnids

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From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Tue, 10 Oct 1995 12:39:36 +0200
Subject: SEM of Daphnids

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From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Tue, 10 Oct 1995 12:39:36 +0200
Subject: SEM of Daphnids

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} To: zaluzec-at-sparc5.microscopy.com
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} Subject: SEM of Daphnids
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From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Tue, 10 Oct 1995 12:39:36 +0200
Subject: SEM of Daphnids

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From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Tue, 10 Oct 1995 12:38:52 +0200
Subject: Ion Mill spare parts ?

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} To: zaluzec-at-sparc5.microscopy.com
} Message-Id: {951010101925.2b1-at-aaem.amc.anl.gov}
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From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Tue, 10 Oct 1995 12:38:52 +0200
Subject: Ion Mill spare parts ?

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Email: Zaluzec-at-MSA.Microscopy.Com
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From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Tue, 10 Oct 1995 12:41:51 +0200
Subject: EPMA standards (continued)

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} From: MICROSCOPY-at-aaem.amc.anl.gov
} Date: Tue, 10 Oct 1995 10:23:13 -0500 (CDT)
} To: zaluzec-at-sparc5.microscopy.com
} Message-Id: {951010102313.2b1-at-aaem.amc.anl.gov}
} Subject: EPMA standards (continued)
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From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Tue, 10 Oct 1995 12:41:51 +0200
Subject: EPMA standards (continued)

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From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Tue, 10 Oct 1995 12:39:57 +0200
Subject: Photo Film Holders Giveaway

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} From: MICROSCOPY-at-aaem.amc.anl.gov
} Date: Tue, 10 Oct 1995 10:20:57 -0500 (CDT)
} To: zaluzec-at-sparc5.microscopy.com
} Message-Id: {951010102057.2b1-at-aaem.amc.anl.gov}
} Subject: Photo Film Holders Giveaway
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From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Tue, 10 Oct 1995 12:39:57 +0200
Subject: Photo Film Holders Giveaway

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From: John Millar :      JJMILL-at-bunyip.ph.rmit.edu.au
Date: Wed, 11 Oct 1995 10:00:15 EST-10
Subject: address

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CAn anyone let me know the email address for David Smith at Arizona
State U, Center for Solid State Science.
Thank you in advance
Cheers
jjm
Professor John J. Millar
Head, Department of Applied Physics
Electron Microscope Unit
RMIT, GPO Box 2476V, MELBOURNE AUSTRALIA 3001
+613 660 2602 fax 613 660 3837
email jjmill-at-rmit.edu.au




From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Tue, 10 Oct 1995 12:37:58 +0200
Subject: Laser Printers & Auger

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} To: zaluzec-at-sparc5.microscopy.com
} Message-Id: {951010101729.2b1-at-aaem.amc.anl.gov}
} Subject: Laser Printers & Auger
} Content-Type: text
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From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Tue, 10 Oct 1995 12:37:58 +0200
Subject: Laser Printers & Auger

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Email: Zaluzec-at-MSA.Microscopy.Com
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From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Tue, 10 Oct 1995 12:40:53 +0200
Subject: Polaroid processing tanks

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} From: MICROSCOPY-at-aaem.amc.anl.gov
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} To: zaluzec-at-sparc5.microscopy.com
} Message-Id: {951010102230.2b1-at-aaem.amc.anl.gov}
} Subject: Polaroid processing tanks
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From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Tue, 10 Oct 1995 12:40:53 +0200
Subject: Polaroid processing tanks

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Microscopy Society of America
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Email: Zaluzec-at-MSA.Microscopy.Com
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From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Tue, 10 Oct 1995 12:41:36 +0200
Subject: SEM/EPMA, carbon coating of samples

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} From: MICROSCOPY-at-aaem.amc.anl.gov
} Date: Tue, 10 Oct 1995 10:23:00 -0500 (CDT)
} To: zaluzec-at-sparc5.microscopy.com
} Message-Id: {951010102300.2b1-at-aaem.amc.anl.gov}
} Subject: SEM/EPMA, carbon coating of samples
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From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Tue, 10 Oct 1995 12:41:36 +0200
Subject: SEM/EPMA, carbon coating of samples

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Email: Zaluzec-at-MSA.Microscopy.Com
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From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Tue, 10 Oct 1995 12:39:47 +0200
Subject: X-ray scanning microscope?

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} From: MICROSCOPY-at-aaem.amc.anl.gov
} Date: Tue, 10 Oct 1995 10:20:42 -0500 (CDT)
} To: zaluzec-at-sparc5.microscopy.com
} Message-Id: {951010102042.2b1-at-aaem.amc.anl.gov}
} Subject: X-ray scanning microscope?
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From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Tue, 10 Oct 1995 12:39:47 +0200
Subject: X-ray scanning microscope?

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Email: Zaluzec-at-MSA.Microscopy.Com
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From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Tue, 10 Oct 1995 12:38:31 +0200
Subject: X-ray scanning microscope?

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} From: MICROSCOPY-at-aaem.amc.anl.gov
} Date: Tue, 10 Oct 1995 10:18:35 -0500 (CDT)
} To: zaluzec-at-sparc5.microscopy.com
} Message-Id: {951010101835.2b1-at-aaem.amc.anl.gov}
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From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Tue, 10 Oct 1995 12:38:31 +0200
Subject: X-ray scanning microscope?

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Microscopy Society of America
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Email: Zaluzec-at-MSA.Microscopy.Com
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From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Tue, 10 Oct 1995 12:40:41 +0200
Subject: TEM: diamond support grids

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} From: MICROSCOPY-at-aaem.amc.anl.gov
} Date: Tue, 10 Oct 1995 10:22:17 -0500 (CDT)
} To: zaluzec-at-sparc5.microscopy.com
} Message-Id: {951010102217.2b1-at-aaem.amc.anl.gov}
} Subject: TEM: diamond support grids
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From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Tue, 10 Oct 1995 12:40:41 +0200
Subject: TEM: diamond support grids

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Nestor J. Zaluzec

Microscopy Society of America
TeleCommunications Office
Email: Zaluzec-at-MSA.Microscopy.Com
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From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Tue, 10 Oct 1995 12:39:09 +0200
Subject: Re: Laser Printers

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} {zaluzec-at-sparc5.microscopy.com} ; Tue, 10 Oct 1995 10:19:21 -0500
} From: MICROSCOPY-at-aaem.amc.anl.gov
} Date: Tue, 10 Oct 1995 10:19:57 -0500 (CDT)
} To: zaluzec-at-sparc5.microscopy.com
} Message-Id: {951010101957.2b1-at-aaem.amc.anl.gov}
} Subject: Re: Laser Printers
} Content-Type: text
}



From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Tue, 10 Oct 1995 12:39:09 +0200
Subject: Re: Laser Printers

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-----------------------------------------------------------
Nestor J. Zaluzec

Microscopy Society of America
TeleCommunications Office
Email: Zaluzec-at-MSA.Microscopy.Com
-----------------------------------------------------------






From: Robert Fisher :      rmfisher-at-u.washington.edu
Date: Tue, 10 Oct 1995 19:13:28 -0700 (PDT)
Subject: X-ray Microscope

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X-Sender: rmfisher-at-homer11.u.washington.edu



Jerome - Roger Johnson just finished a thesis here
on a conversion of an SEM for x-ray tomography
and microscopy. We had a short paper at EMSA?MSA
Boston in 1992.

Bob Fisher




From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Tue, 10 Oct 1995 12:39:01 +0200
Subject: Re: UTW vs Be Windows

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} Subject: Re: UTW vs Be Windows
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From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Tue, 10 Oct 1995 12:39:01 +0200
Subject: Re: UTW vs Be Windows

Contents Retrieved from Microscopy Listserver Archives
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-----------------------------------------------------------
Nestor J. Zaluzec

Microscopy Society of America
TeleCommunications Office
Email: Zaluzec-at-MSA.Microscopy.Com
-----------------------------------------------------------






From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Tue, 10 Oct 1995 12:38:07 +0200
Subject: UTW vs Be Windows

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} From: MICROSCOPY-at-aaem.amc.anl.gov
} Date: Tue, 10 Oct 1995 10:17:42 -0500 (CDT)
} To: zaluzec-at-sparc5.microscopy.com
} Message-Id: {951010101742.2b1-at-aaem.amc.anl.gov}
} Subject: UTW vs Be Windows
} Content-Type: text
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From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Tue, 10 Oct 1995 12:38:07 +0200
Subject: UTW vs Be Windows

Contents Retrieved from Microscopy Listserver Archives
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-----------------------------------------------------------
Nestor J. Zaluzec

Microscopy Society of America
TeleCommunications Office
Email: Zaluzec-at-MSA.Microscopy.Com
-----------------------------------------------------------






From: zaluzec-at-Microscopy.Com (Nestor J. Zaluzec)
Date: Tue, 10 Oct 1995 22:30:10 -0500
Subject: Microscopy is back on-line with some major changes!

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************************************************************
Welcome to
************************************************************

Microscopy-at-MSA.Microscopy.Com

************************************************************
Hosted and Sponsored by The Microscopy Society of America (MSA)
as a Free Service to the World-Wide Microscopy & Microanalysis Community
************************************************************


G'day Subscribers....... (Well at least I hope it is day-time, when you
read this )

It looks as if we are now back on-line, and as the subject title and the above
banner indicate some major changes have occurred during the last few days.
You might even say we now have a new face, well at least a new envelope !

Firstly, as most of you know I had been running the Microscopy Listserver
at ANL for just over 2 years, basically without any real support, save my
time and
some electricity. As a consequence it was becoming harder and harder to keep
things running properly, let alone efficiently.

At this time it is my pleasure to officially announce that the Microscopy
Listserver has
moved (hopefully, nearly transparently to you but obviously with a bit of
work for me)
to a new address and with a new sponsor.

MSA at their 1995 Winter/Spring Council meeting, voted to establish a full
internet site
for the society and it's members and to host the Microscopy Listserver as a
FREE service to the microscopy and microanalysis community worldwide. It
has taken some time to get everything running, but as of last night, the
listserver is now relocated and functioning with a modicum of success. I
still basically do the majority of the work (gratis), however, we now have
a new
workstation (Sun Sparc5), software, and as I mentioned an offical internet
site.
The server will still need some work, but we now have the resources to
improve the system and make it more user-friendly.

For example, with the new configuration of header (i.e. the envelope of
this Email message), errors and undeliverables should now no-longer be
returned to the originator, but are sent to the SysOp
( Ijust need more mail, right?). In addition, I will be bringing up
on-line some of the more usual features characteristic of listserver
software in the coming weeks . I will post the information to to the list
as the options become functional.


I would encourage you to send thanks to MSA Council for saving the
listserver from
a slow death, as the hardware and software configurations at ANL were
becoming increasing difficult to maintain and as some of you know were
beginning to get particuliarly ornery.
From my own perspective, my thanks go to all the members of council for
establishing
this site, and a special thanks to two members who have been especially
supportive
namely- Ron Gronsky of UC Berkeley and Ron Anderson of IBM.

The general MSA Email Address is:

MSA-at-MSA.Microscopy.Com

while the business office can be reached at:

MSABusinessOffice-at-MSA.Microscopy.Com.

Additional information about MSA, the society and it's operations can be
found at the MSA WWW site:

http://WWW.MSA.Microscopy.Com

MSA also mirrors the MMSLIB (Microscopy & Microanalysis Software Library)
anonymous ftp site at:

FTP.MSA.Microscopy.Com

and as you already know the Microscopy Listserver at:

Microscopy-at-MSA.Microscopy.Com

Mail from the old server site (Microscopy-at-AAEM.AMC.ANL.GOV) will be
automatically
forwarded to the new server site for awhile, but please change your local
aliases and/or
shortcuts, so that your mail goes directly to the MSA site instead of
channeling through ANL.

As I mentioned, there will still be a few growing pains, but the hard part
should be basically done.

------------------------------------------------------------------------------

In addition to the move from the ANL to the MSA site, the listserver has
passed another milestone this weekend, the 3,000th subscription request
arrived, not bad for only 2 years of operation. The lucky winner of a beer
should he and I ever meet is:

Russell J. Wilson
Analytical Electron Microscope Facility
Queensland University of Technology
Garden Point
G.P.O Box 2434
Brisbane Qld. 4001.

Russell, it's your job to make sure that a cool slab of tinney's is
around, but I'll buy!

--------------------------------------------------------------------------------

BTW if you are a triva buff or just curious the list sends Email to
Microscopists in
39 countries around the world. Over 5600 Email messages have been
submitted
to Microscopy over the period Oct 1, 1993 to Oct 1, 1995. Not all
of them have
been gems, but there have been occasional useful bits of info......

----------------------------------------------------------------------------
-----


...... Nestor

Your Friendly Neighborhood SysOp






From: Stuart McClure :      Stuart.McClure-at-adl.soils.csiro.au
Date: Wed, 11 Oct 1995 14:28:19 +1000
Subject: Re: X-ray scanning microscope?

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Message-Id: {199510110507.AA21791-at-shrike.adl.soils.csiro.au}
X-Sender: mcc332-at-shrike.adl.soils.csiro.au
X-Mailer: Windows Eudora Pro Version 2.1.2
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

If this is any help to you. There was a paper that I remember seeing about
10 yrs ago that used a thin metal film to generate xrays from the electron beam
which were then used as a point source to pass through the specimen
onto xray film. Magnification set by the source-specimen :specimen-film
distance.
ie e
e
e
m-metal-m
x
x x
ss-specimen-sss
x x
x x
fffff--film--fffff

I can't at this moment find the reference but I can have a
good dig if you want.

stuart

} }
} } Several years back I read about a group that interposed an X-ray source
} } within a STEM or SEM. Electrons hit the target, generating X-ray's which
} } could be used to visualize the specimem producing both an electron image
} } and an X-ray image of the same sample. I have searched the literature and
} } cannot find a reference to this. Does anyone know of a reference or the
} } existence of such a technology or am I just getting senile?
} } Your help and assistance with either of these two issues (the scope or my
} } senility) would be appreciated.
} }
} } Thanks-

} } Jay Jerome
* jjerome-at-isnet.is.wfu.edu *
} } **************************************************************



---------------------------------------------------------------------------
Stuart G. McClure, | Post small : P.B.#2, Glen Osmond,
CSIRO Division of Soils, | Sth Australia, AUSTRALIA, 5064.
Adelaide Laboratories, | Post large : Waite Rd, Urrbrae,
SA, Australia | Sth Australia, AUSTRALIA, 5064.

Phone: (08) 303-8484 International use +61-8- instead of (08)
Fax: (08) 303-8550
Email: Stuart.McClure-at-adl.soils.csiro.au
---------------------------------------------------------------------------





From: Krzysztof Hubner :      zahubner-at-cyf-kr.edu.pl
Date: Wed, 11 Oct 1995 10:09:37 +0100 (MET)
Subject: acoustic microscope

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Dear Friends,

I am looking for names and address (email or fax) of companies who
make acoustic microscopy for the materials sciences research.

Best regards for

Krzysztof Jan Hubner {zahubner-at-cyf-kr.edu.pl}
Foundry Research Institute
Research Department - Structural and Physical Research Laboratory
Zakopianska 73 Call +48 12 605022 ext. 356
30-148 KRAKOW - POLAND Fax +48 12 665478 :-)





From: Ray Hicks :      rh208-at-cus.cam.ac.uk
Date: Wed, 11 Oct 1995 10:54:01 +0100 (BST)
Subject: Re: TIFF FILE CONVERTER FOR PC

Contents Retrieved from Microscopy Listserver Archives
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Hi,
You can try using graphicConvertor a shareware program that lets you
convert several mac/ibm formats. It's available from several sites, you
can look in zippy.nimh.nih.gov /pub/nih-image/programs using anonymous
ftp. If its not in programs its in one of the subdirectories of nih-image.
By the way NIH-Image itself is well worth a look, it has some import
capabilities (but doesn't handle all types of TIFF), and it allows a
range of image analysis techniques as well as 3-D reconstruction etc.

Ray




From: Lcapel-at-eliovac.com.ar
Date: Wed, 11 Oct 1995 08:44:27 +0000
Subject: subscribe

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Lilian Capel
Eliovac S.A.
Av. Coronel Uzal 4223/35
1636 Olivos
Buenos Aires
Argentina

Tel: 54-1-798-0110
Fax: 54-1-799-9575
E-mail: Lcapel-at-eliovac.com.ar




From: mgeorge-at-dubois.fisk.edu (Michael George)
Date: Wed, 11 Oct 1995 08:59:16 -0500
Subject: subscribe

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I would like to subscribe.


Michael A. George, Ph.D.
*******************************************
Research Assistant Professor
Department of Physics
Fisk University
Nashville, TN 37208

Office: (615)329-8525 Lab: (615)329-8736
Fax: (615)329-8634 mgeorge-at-dubois.fisk.edu





From: Donald Lovett :      lovett-at-Trenton.EDU
Date: Wed, 11 Oct 1995 10:20:20 -0400 (EDT)
Subject: Looking for a used TEM.

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To update and clarify my message of yesterday, we are looking to
-} purchase {- a used H-600. We are pretty much wed to the idea of
staying with Hitachi products.

Thank you for responses received to date.

Donald L. Lovett e-mail: lovett-at-trenton.edu
Asst. Professor, Dept. of Biology voice: (609) 771-2876
Trenton State College, NJ 08650-4700 fax: (609) 771-2674






From: OptoMech-at-aol.com
Date: Wed, 11 Oct 1995 12:58:19 -0400
Subject: Looking for a used TEM.

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on selling a used microscope? We have a light microscope
for sale and want to check first before we break any rules! I have seen
SEM's and the such offered and want to see if it is OK .

M. Davis
410-265-5873 phone/fax




From: imgp-at-mbimp1.mbl.tno.nl (Kees van der Wulp)
Date: Wed, 11 Oct 1995 11:41:35 +0100 (MET)
Subject: Request : Molecular Dynamics e-mail address

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Dear colleagues,

Does anybody know the e-mail address of :

Molecular Dynamics in Sunnyvale, CA

Please send your answer directly to : vanderwulp-at-voeding.tno.nl

Thanks for the effort.

Kees
--
Kees van der Wulp

TNO - Nutrition & Food Research Institute INTERNET : vanderwulp-at-voeding.tno.nl
Division : Toxicology VOICE : +31 15 843101
Department : Genetic Toxicology FAX : +31 15 843989
PO-Box 5815 General TNO Info : http://www.tno.nl
2280 HV RIJSWIJK (NL)
THE NETHERLANDS





From: levin-at-ecsuc.ctstateu.edu (Martin Levin)
Date: Wed, 11 Oct 1995 08:09:10 -0500
Subject: Sputter Coater

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To anyone with a sputter coater laying around:

We are a small, state, liberal arts university with a very limited budget.
I just had an SEM donated to our department and am now in need of a sputter
coater and critical point dryer. If anyone has either of these pieces of
equipment not currently being used or knows where I could get them
(preferably as a donation) please let me know. Thank you.

Marty Levin
Department of Biology
Eastern Connecticut State University
Willimantic, CT 06226






From: wfritz-at-iastate.edu
Date: Wed, 11 Oct 1995 14:45:08 -0600
Subject: subscribe

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From: azriel gorski :      azrielg-at-cc.huji.ac.il
Date: Wed, 11 Oct 1995 14:09:11 +0200 (IST)
Subject: Do I want to be the first to ask?

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11. October. 1995

First, I want to thank Nestor (Our Friendly Neighborhood SysOP) for all
his untiring efforts. I would also like to thank MSA for agreeing to
support the list. (Very tongue in cheek) I am sure both will receive
their just rewards.

BUT... to be the first of many to ask this.... with the change of
listserver.. how does one Subscribe/Unsubscribe. That faciously, is a
request for information on the correct address of the Listserver and the
commands needed to get it to do what we would like it to do.

Again thank you to you Nestor for your time and effort.

Shalom from Jerusalem,
Azriel Gorski





From: INGRAM-at-RTI.ORG
Date: Wed, 11 Oct 1995 09:42:59 -0400 (EDT)
Subject: Re: X-ray scanning microscope?

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Greetings!
The reference(s) to which you refer Jay are:

Middleman L.M. and Geller J.D. Scanning Electron Microscopy (1976),
I, 171 -179
Eckert R. Scanning, 8, 232 -238, (1986)

I've tried it - and it definitely works ( not unexpectedly! ). The problem
is spatial resolution; now, however, there has been significant improvement
in x-ray focusing optics and several groups ( including ours ) are in various
stages of constructing useful ( hopefully! ) scanning x-ray fluorescence
microscopes.

Cheers!
Peter Ingram
SEND






From: Liang, Long :      LLIANG-at-is.arco.com
Date: 11 Oct 1995 14:21:14 CST
Subject: EPMA--OsO4 staining on rocks

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Message-Id: {MACMS.LLIANG.751918140095284FMACMS-at-IS.ARCO.COM}


Dear Microscopists,

Does anyone have used heavy metal staining (OsO4) on rock samples
(prepared as polished sections) in order to detect the occurrence of
organic material ? Where could I find the staining procedure ?
Thanks in advance.

Long Liang
ARCO EPMA/SEM Lab
Plano, TX
lliang-at-is.arco.com








From: geosclmr-at-showme.missouri.edu (Lou Ross)
Date: Wed, 11 Oct 1995 14:07:04 -0600
Subject: ARL EMX-SM

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Message-Id: {199510111906.OAA13380-at-mail.missouri.edu}
X-Sender: geosclmr-at-pop.missouri.edu
Mime-Version: 1.0
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Hi,

We will be dismantling an EMX-SM microprobe in the next few weeks.
It hasn't been used in 2 years but has been kept under vacuum. Along with
the microprobe itself, I have plenty of spare components and parts. Anyone
is welcome to all or any of the components for the cost of shipping.

Contact me directly if interested.

Thanks,
Lou Ross

101 Geological Sciences Bldg.
University of Missouri
Columbia, MO 65211
(314) 882-4777, 882=5458 fax






From: Peter.Reynders-at-CON1.EMD.MERCK.DBP.de
Date: Wed, 11 Oct 1995 09:47:00 -0500
Subject: SUBSCRIBE

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Wed, 11 Oct 1995 09:07:32 -0500
X400-Received: by /PRMD=ESnet/ADMD= /C=US/; Relayed;
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SUBSCRIBE




From: ldm2-at-apollo.numis.nwu.edu (L.D.Marks)
Date: Wed, 11 Oct 1995 08:18:35 -0500
Subject: Public Domain Icons

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I have finaly taken the time to organize my own collection of
icons, and collect a few microscopy related ones from other
sources. There are also a few small, and well colored icons
that I have found to be very useful for teaching.

The appropriate link is:
http://risc1.numis.nwu.edu/internet/Icons

Notes:
1) Some of the directories are quite large and may be
very slow if you have a slow modem connection.
2) Contributions are welcome - please contact me at
l-marks-at-nwu.edu .
3) I am receptive to the idea of mirroring some of these
icons in Europe and Asia to improve access.

Acknowledgements

I would like to thank:
Pierre-Henri Jouneau, Centre Interdepartemental de Microscopie Electronique
David Dryden, , University of Melbourne
Daniel L. Callahan Department of Mechanical Engg. and Materials Science, Rice University
JEOL, USA
Nissei Sangyo Canada, Inc




From: Tam Kin Yip :      tam-at-physchem.ox.ac.uk
Date: Wed, 11 Oct 1995 23:14:25 +0100 (BST)
Subject: subscribe

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From: f.m.haug-at-basalmed.uio.no (Finn-Mogens Haug)
Date: Wed, 11 Oct 1995 14:50:18 +0200
Subject: Fow Cytometry Standards phone/fax no?

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Message-Id: {199510111247.NAA05175-at-pons.uio.no}
X-Sender: finnmog-at-pons.uio.no
X-Mailer: Windows Eudora Version 1.4.3
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Their adress was cited by the Thomas register as
Flow Cytometry Standards Corporation
513 Hostos Avenue, Ste. B3
P.O. Box 194344
San Juan, Puerto Rico 00919

but TR could not find any phone/fax numbers. Thanks in advance
to those who send the numbers by direct e-mail (and I will post the
numbers to the list, once). Regards from





Finn-Mogens Haug

University of Oslo
Institute of basic medical sciences
Department of anatomy E-mail: f.m.haug-at-basalmed.uio.no
Box 1105 Blindern Phone : +47 22 85 12 67
N-0317 Oslo, NORWAY Fax : +47 22 85 12 78






From: Krzysztof Hubner :      zahubner-at-cyf-kr.edu.pl
Date: Wed, 11 Oct 1995 10:09:37 +0100 (MET)
Subject: acoustic microscope

Contents Retrieved from Microscopy Listserver Archives
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I do not have addresses or emails ids readily available but I can give you
companies who make them:

Sonoscan
Hitachi
Olympus
Sonix

Hope this helps.

--- Forwarded mail from Krzysztof Hubner {zahubner-at-cyf-kr.edu.pl}




Dear Friends,

I am looking for names and address (email or fax) of companies who
make acoustic microscopy for the materials sciences research.

Best regards for

Krzysztof Jan Hubner {zahubner-at-cyf-kr.edu.pl}
Foundry Research Institute
Research Department - Structural and Physical Research Laboratory
Zakopianska 73 Call +48 12 605022 ext. 356
30-148 KRAKOW - POLAND Fax +48 12 665478 :-)



---End of forwarded mail from Krzysztof Hubner {zahubner-at-cyf-kr.edu.pl}

--
Regards,

Tom Taylor {ttaylor-at-msai.mea.com}




From: Michael Rock :      merock-at-u.washington.edu
Date: Wed, 11 Oct 1995 15:50:26 -0700 (PDT)
Subject: Diamond for sale

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I have an unused 3.0 mm diamond knife (ultramicrotomy) for sale if anyone
is interested...reply directly.
Mike Rock




From: Microscopy-request
Date: Wednesday, October 11, 1995 10:09AM
Subject: acoustic microscope

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Dear Friends,

I am looking for names and address (email or fax) of companies who
make acoustic microscopy for the materials sciences research.

Best regards for

Krzysztof Jan Hubner {zahubner-at-cyf-kr.edu.pl}
Foundry Research Institute
Research Department - Structural and Physical Research Laboratory
Zakopianska 73 Call +48 12 605022 ext. 356
30-148 KRAKOW - POLAND Fax +48 12 665478 :-)





From: dstokke-at-siu.edu (Douglas D. Stokke)
Date: Wed, 11 Oct 1995 12:10:56 -0500
Subject: PTL Pro 100 Macro Scanning System

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Greetings:
I just received the RMS Proceedings, vol. 30 part 3 (Sept. 1995). The
article on page 194 describes the "PTL Pro 100 Macro Scanning System" but
there is no information on who manufacturers or distributes the system. Any
information would be most appreciated.
Thanks,
Doug
*****************
Douglas D. Stokke
USDA Forest Service
North Central Forest Experiment Station
Forestry Sciences Lab, SIU-C
Carbondale, IL 62901-4630
PH# 618-453-2920
FAX 618-453-2911
e-mail: dstokke-at-siu.edu
Forest Service DG: D.Stokke:S23L01A





From: Fermin, Cesar :      fermin-at-TMC.Tulane.Edu
Date: 11 Oct 1995 10:58:08 -0500
Subject: Image convert

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Message-Id: {n1398712941.61314-at-msmail.tmc.tulane.edu}

Image convertion:
About the discussion on image convertion, the Share ware (usesr should pay
small fee to keep this type of service upcoming) {Graphic Converter} can be
downloaded from
http://hyperarchive.lcs.mit.edu/HyperArchive/Archive/gst/grf/graphic-converter-215.hqx
Make sure that you have a working copy of {Stuff it} (also share ware) in you
computer so that the file is automatically decompressed from its binhex
format.
Graphic Converter is truly outstanding for its price ($35.00).

******************************************************************
*Cesar D. Fermin, Ph.D \|T|/ Fax (504) 587-7389 *
*Tulane Medical School /|M|\ Voice Mail (504) 584-2618 *
*Pathology/SL79 \|C|/ Secretary (504) 584-2436 *
*New Orleans La 70112-2699 /|*|\ Lab/Techn. (504) 584-2521 *
* {Fermin-at-tmc.tulane.edu} Dir. Morphological Services *
******************************************************************





From: Thingfetch-at-aol.com
Date: Wed, 11 Oct 1995 20:24:31 -0400
Subject: subscribe

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Please add me to your mail list.
Thank you,
Rick Schultz




From: Fermin, Cesar :      fermin-at-TMC.Tulane.Edu
Date: 11 Oct 1995 13:43:57 -0500
Subject: Syquest-SCSI/DOS-MAC

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Message-Id: {n1398702733.77870-at-msmail.tmc.tulane.edu}

I use Syquest removable media (44MB each) for image storage in the Mac and
the PC. I purchased DOS MOUNTER from DAYNA Communication, inc and can mount
easily floppy DOS-MAC. Are there any drivers out there (software?) that would
allow me to bring a DOS formatted Syquest to the MAC without having to update
the driver every time is inserted across plataforms? Thanks in advance.

******************************************************************
*Cesar D. Fermin, Ph.D \|T|/ Fax (504) 587-7389 *
*Tulane Medical School /|M|\ Voice Mail (504) 584-2618 *
*Pathology/SL79 \|C|/ Secretary (504) 584-2436 *
*New Orleans La 70112-2699 /|*|\ Lab/Techn. (504) 584-2521 *
* {Fermin-at-tmc.tulane.edu} Dir. Morphological Services *
******************************************************************




From: John Millar :      JJMILL-at-bunyip.ph.rmit.edu.au
Date: Thu, 12 Oct 1995 09:48:25 EST-10
Subject: Smith address

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Thank you to all those who replied. Received and understood.
Cheers
jjm
Professor John J. Millar
Head, Department of Applied Physics
Electron Microscope Unit
RMIT, GPO Box 2476V, MELBOURNE AUSTRALIA 3001
+613 660 2602 fax 613 660 3837
email jjmill-at-rmit.edu.au




From: John Millar :      JJMILL-at-bunyip.ph.rmit.edu.au
Date: Thu, 12 Oct 1995 09:48:25 EST-10
Subject: Smith address

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Thank you to all those who replied. Received and understood.
Cheers
jjm
Professor John J. Millar
Head, Department of Applied Physics
Electron Microscope Unit
RMIT, GPO Box 2476V, MELBOURNE AUSTRALIA 3001
+613 660 2602 fax 613 660 3837
email jjmill-at-rmit.edu.au




From: dsimpson-at-warwick.net (Derek Simpson)
Date: Wed, 11 Oct 1995 11:51:21 -0400
Subject: X-Ray Scanning Microscope

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In the late 70's I saw this method used at a hospital in Calgary
Canada. They had modified a Cambridge S180. The stage had a target for X-ray
generation with the specemin (a hamster eyeball) beneath it. Below that was
a minature 35mm camera they had manufactured. I hope this will help you.
Check with Cambridge / Leica for S180 user in Calgary.


} } } Several years back I read about a group that interposed an X-ray source
} } } within a STEM or SEM. Electrons hit the target, generating X-ray's which
} } } could be used to visualize the specimem producing both an electron image
} } } and an X-ray image of the same sample. I have searched the literature and
} } } cannot find a reference to this. Does anyone know of a reference or the
} } } existence of such a technology or am I just getting senile?
} } } Your help and assistance with either of these two issues (the scope or my
} } } senility) would be appreciated.
} } }
} } } Thanks-
}
} } } Jay Jerome
} * jjerome-at-isnet.is.wfu.edu *
} } } **************************************************************
}
Regards
Derek Simpson





From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Wed, 11 Oct 1995 11:03:29 -0500
Subject: Yo, Nestor!

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Message-Id: {199510111605.LAA27683-at-bcm.tmc.edu}
X-Sender: joiner-at-bcm.tmc.edu
X-Mailer: Windows Eudora Version 2.1.1
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Congrats and kudos on the move of the listserver. We all appreciate the work
that you've done. HOWEVER.....You were waiting for that, weren't
you?....Your message: "Microscopy is back on-line with some major changes!"
came across, to my machine at least, in what appears to be two parts. The
first ended with your paragraph asking us to thank the MSA Council, and no
signature. The second part came as a second message begining in mid-sentence
without an envelope. I knew it was from you because of the signature at the
end. There appears to be a chunk of message missing from the middle. I
believe that you said in this message that the new address for messages to
the listerver is: microscopy-at-msa.microscopy.com. Is this correct? But what I
got did not specify an address for subscribe/unsubscribe requests. Is there
one? I would like to wait to change my aliases until I have confirmed these
addresses.




Joiner Cartwright, Jr., Ph.D.
Director, Electron Microscopy
Department of Pathology, Rm.286-A
Baylor College of Medicine
One Baylor Plaza
Houston, Texas 77030 U.S.A.
tel.: (713)798-4658
FAX: (713)798-3945
joiner-at-bcm.tmc.edu
Compuserve: 71555,1206





From: wesaia-at-iastate.edu (Warren Straszheim)
Date: Wed, 11 Oct 1995 10:45:25 -0500
Subject: TIFF FILE CONVERTER FOR PC

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From: wesaia-at-iastate.edu (Warren Straszheim)
Date: Wed, 11 Oct 1995 10:45:25 -0500
Subject: TIFF FILE CONVERTER FOR PC

Contents Retrieved from Microscopy Listserver Archives
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From early on I noticed that not all apps treat TIFF images the same.
Pagemaker under Windows could read my files while Word could not. I had
similar experience on the MAC. Some MAC apps could read my images while
others could not. A lot depended on the converters in the app. It was not a
Mac ves. PC issue, but rather what the TIFF structure was. Both Mac and PC
apps _should_ be able to read the same TIFF file. For a while I used Graphics
Workshop to convert the file to a new TIFF file and the resulting file
sometimes worked in more apps.

I am not sure if I completely understand the workings now, but I have gotten
most of the tags straightened out (TIFF = Tag Image File Format). If I
recall, I am now overspecifying the image with the tags I now include (e.g.,
(size of image and hor.-dimension and vert.-dimension). But if that is what
it takes to keep more apps happy, then so be it.

In summary, I do not have any suggestion for converters. I would be
interested in what you find out. I suppose that it may take a reputable
commercial product to be robust enough to handle all apps. But even then, I
would test the images on the apps of concern.





From: xxie-at-lsuvm.sncc.lsu.edu (Xiaogang Xie)
Date: Wed, 11 Oct 1995 18:26:00 -0600
Subject: Re: X-ray scanning microscope?

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Message-Id: {199510112322.SAA02917-at-Sparc5.Microscopy.Com}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

} } Several years back I read about a group that interposed an X-ray source

A book titled "Scanning electron microscopy and x-ray
microanalysis" written by J. I. Goldstein et al. has a chapter about this
(2nd ed. 1992).

Xiaogang

****************************************
* Xiaogang Xie *
* Department of Geology and Geophysics *
* Louisiana State University *
* Baton Rouge, LA 70803 *
* Office (504)388-2240 *
* Fax (504)388-2302 *
****************************************






From: f.lawrence-at-qut.edu.au (Felicity Lawrence)
Date: Thu, 12 Oct 1995 12:02:27 +1000
Subject: Obj lenses & DAPI

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Hi Everybody!

We have been given a DAPI filter cube to trial prior to purchase. I have
two questions regarding its use :

1. Different objective lenses give different images. Why?
2. General uses for a DAPI filter cube. Can we justify the cost?

-------
1. When we observe our specimen (macrophages within lung - cryostat
sections) using a 40x Pl Fluotar objective 1.00-.50 oil (LEICA lens), our
images are superb : clear, crisp, pretty. This is the case both with oil
immersion and just dry viewing using this lens.

The problem occurs when we view our sample with our 60x Pl Apo objective 1.4
oil (LEICA lens). The image becomes non-existent or ghost-like. A pale
"shadow" only is seen, with no detail visible at all. However, viewing with
green and blue excitation lines (fluorescence microscope) continues to give
excellent (although non-specific fluorescent) images on both the 40x and 60x
objectives.

I discussed our problem with LEICA. At first thought, the explanation they
proposed was that the 60x Pl Apo was giving autofluoresence from the glass
components of the objective lens following excitation with ultraviolet light
(selected by the DAPI filter). This then masked the sample fluorescence to
the extent that no image was obtained. The longer wavelengths (green and
blue lines) would not cause this autofluorescence.

In comparison, the Pl Fluotar 40x objective could cope with a larger range
of wavelengths, hence this problem was not observed with this lens.

IS THIS A REASONABLE ASSUMPTION? HAVE OTHER PEOPLE EXPERIENCED SIMILAR
DIFFICULTIES?
-------
2. Our application is visualisation of marcophage particles in lung
tissue. Macrophages autofluoresce but we are trying to locate the presence
of benzopyrene within the cells. Benzopyrene excites at 380nm and emits at
430nm. The DAPI filter seems a reasonable choice (and was recommended by
this discussion list).

WHAT ARE OTHER PEOPLE USING DAPI FILTERS/STAINS FOR? ARE THERE MANY OTHER
GENERAL APPLICATIONS FOR DAPI THAT COULD HELP JUSTIFY THE PURCHASE PRICE OF
~$650 (AUSSIE DOLLARS)?

Many thanks for your responses,

Felicity Lawrence
Analytical Electron Microscopy Facility, QUT
Brisbane, Australia





From: Stefanie Harvey :      maddr-at-netcom.com
Date: Wed, 11 Oct 1995 21:42:14 -0700 (PDT)
Subject: subscribe

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From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-aaem.amc.anl.gov
Date: Wed, 11 Oct 1995 23:41:21 -0500 (CDT)
Subject: How to Subscribe/Unsubscribe

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Azriel & anyone else that is curious....

For the moment, the procedure to subscribe and/or unsubscribe remains
the same. Just send a Email message to:

Listserver-at-MSA.Microscopy.Com

in the body of the message type the following

Subscribe Microscopy {YourUserID-at-YourDomain}
or
Unsubscribe Microscopy {YourUserID-at-YourDomain}

just like before. The only difference is the MSA Internet Address, and yes
the old address for this also forwards from AAEM.AMC.ANL.GOV to
MSA.Microscopy.Com.

I need a bit of time to prepare new documentation and the like,
but when it's ready you will all get a copy. For the present
most things run in a similiar procedure to the old system, except
possibly fewer error messages. BTW the "rules and etiquette" on this
list remain the same as before, remember it's the same list, just a new address.

You will all also continue to see the random subscribe/unsubscribe messages
which are incorrectly posted to the "Microscopy" address instead of
the "Listserver" address. We've already had several in the last few
days. That I can't do anything about, unless the
list becomes "Moderated" which means someone must read and approve each
and every message. Sorry folks, but there is no way I have time to do
that...

You should also be able to now easily recognize Email from the listserver
by looking at the Sender's address in your Email program. Each mail
message which is "distributed" by this server has a new envelope
prepended to it. Thus, all mail from here will now have a From: line
which looks like:




From: header to automatically sort/filter out all mail from Microscopy
Date: Wed, 11 Oct 1995 14:09:11 +0200 (IST)
Subject: How to Subscribe/Unsubscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

while a few lines down in the header you will be able to
locate the name of the person that posted the original
message something like this


If you have a smart Email client program, you can use this new

You might also notice that the Domain name appears as Sparc5.Microscopy.Com.
instead of MSA.Microscopy.Com. This is a fluke of the SUN system
software. The computer which I am running on is called locally Sparc5
and the sendmail program appends the Domain name of just Microscopy.Com.
which is the offical domain for the site. The name MSA.Microscopy.Com
is officially registered to this particuliar node (206.69.208.10) but
so is the local name of Sparc5. It appears that the local name takes
precedence on mail being sent out. To change the local name will mean
a reconfiguration of all accounts on the system and I don't have
the time for that. I'll figure out a way of cleaning this up eventually. The
sender name of "Microscopy-request" has to do with the alias
system for the list and the way I've set up the initial system.


Later.... Nestor
Your Friendly Neighborhood SysOp







From: Mr I.M. Ross :      rossfile-at-LIVERPOOL.AC.UK
Date: Thu, 12 Oct 1995 11:12:27 +0100 (BST)
Subject: subscribe to newsgroup

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From: Robert Judd 7590-2659 :      robert.judd-at-bggrc.co.uk
Date: Thu, 12 Oct 1995 11:10:00 +0000 (GMT)
Subject: unsubscribe

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Mr-Received: by mta PGRV06; Relayed; Thu, 12 Oct 1995 12:20:45 +0000
Alternate-Recipient: prohibited
Disclose-Recipients: prohibited

unsubscribe





From: James S MArtin :      James.S.Martin-at-williams.edu
Date: Thu, 12 Oct 1995 09:20:20 -0400 (EDT)
Subject: air filtration for microscopy lab

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I am beginning to consider low-cost steps which we can take to further
reduce the number of airborne (nuisance) particles in our optical and
infrared microscopy lab (approx. 2400 cu. ft, 240 sq. ft.). This is a
desire, not a necessity, so cost is a _big_ factor. I see portable air
cleaners and bench clean air hoods as two possible options.

Any others? Does anyone have sufficient experience with portable air
cleaners to make recommendations? Does anyone have a nifty design
for low-cost self-built bench clean air hoods?

Thanks.

James Martin





From: Richard E. Edelmann :      EDELMARE-at-CASMAIL.MUOHIO.EDU
Date: Thu, 12 Oct 1995 10:15:53 -500
Subject: looking for well slides

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Does anyone out there know where a person might be able to locate at
source for light microscopy 'well - slides'?





From: Steven W. Miller :      73150.2217-at-CompuServe.COM
Date: 12 Oct 95 09:43:28 EDT
Subject: Edwards 306 D.P. fluids

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In response to Ritchie Sims,
I was under the impression that most Edwards 306 diffstak pumps used Santovac 5.
BE SURE to check this out with Edwards before proceeding. Santovac 5 takes a
lot higher heat to operate than DC704, they are not mutually interchangeable.

Operating a silicone oil at a heat much above its designed range is dangerous
not just from a contamination point of view but could result in an explosion or
fire.

You can operate Santovac 5 in a pump built for DC704, you will just suffer
slower pumping speeds.

Steve Miller
Phone 708-698-4210
Fax 708-696-2541





From: Nina S Allen :      nallen-at-unity.ncsu.edu
Date: Thu, 12 Oct 1995 12:03:14 -0400 (EDT)
Subject: Re: Obj lenses & DAPI

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CONFOCAL-at-UBVM.cc.buffalo.edu

Dear Felicity:
Your Leica lens may not transmit in the region of excitation as well as
your other lens.
(My best guess).

Dapi filter is very useful. You can stain DNA in nucleii and
mitochondria with the dye DAPI, lignin in plant cells is best observed
with the Dapi filter and there are many other uses.
Regards, Nina Allen




From: Owen P. Mills :      opmills-at-mtu.edu
Date: Thu, 12 Oct 1995 08:39:20 -0400
Subject: Re: ARL EMX-SM

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Hi Lou,

So you're getting rid of the ARL. Have you picked up another probe?

Owen At 02:07 PM 10/11/95 -0600, Lou Ross wrote:
} Hi,
}
} We will be dismantling an EMX-SM microprobe in the next few weeks.
} It hasn't been used in 2 years but has been kept under vacuum. Along with
} the microprobe itself, I have plenty of spare components and parts. Anyone
} is welcome to all or any of the components for the cost of shipping.
}
} Contact me directly if interested.
}
} Thanks,
} Lou Ross
}
} 101 Geological Sciences Bldg.
} University of Missouri
} Columbia, MO 65211
} (314) 882-4777, 882=5458 fax
}
}
}
Owen P. Mills
Michigan Technological University
Metallurgical & Materials Engineering
Rm 512 MME Building
Houghton, MI 49931
906-487-2002
906-487-2934 FAX
opmills-at-mtu.edu





From: tom thielen :      thielent-at-acad.winthrop.edu
Date: Thu, 12 Oct 1995 09:52:25 -0400 (EDT)
Subject: Bio-Rad -> XEVA

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Hello all!

I am having trouble converting a stack generated by a Bio-Rad confocal to
a format the XEVA visual studio recognizes. Any suggestions?

Thanks in advance,
Tom Thielen

*******************************************************************************
*Tom Thielen "People who make sweeping generalizations are stupid!" *
*Pre-Med Major *
*Winthrop University thielent-at-lurch.winthrop.edu *
*******************************************************************************








From: Mike Tymianski :      mike_t-at-camtwh.eric.on.ca
Date: 12 Oct 1995 14:24:27 -0400
Subject: dual c-mount camera adaptors

Contents Retrieved from Microscopy Listserver Archives
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To: microscopy:;

I'm looking for a dual adaptor for my inverted Nikon Diaphot. I need
to attach two CCD cameras to the camera port simultaneously. The only
product I'm aware of costs an arm and a leg (produced by Nikon, of
course). All I need is an adaptor that has room for a dichroic mirror
and a couple of emission filters, with standard C-mount threads.

If anyone knows of a source for this type of device, I'd appreciate
it.

Thanx
Mike Tymianski
Toronto, CANADA
mike_t-at-camtwh.eric.on.ca
(416) 503-5868
.





From: X.m. Burany :      burany-at-sfu.ca
Date: Thu, 12 Oct 1995 07:29:45 -0700 (PDT)
Subject: Be Sample holder

Contents Retrieved from Microscopy Listserver Archives
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Greetings!

We are looking for a new or used berylium double tilting sample holder for
JEOL JEM 2000FX TEM. Please let me know the price.

I greatly appreciate for your help.

Sandy Burany, PhD
Dept. of Physics and Astronomy
University of Delaware
Newark, DE 19716
(302) 831 3515




From: X.m. Burany :      burany-at-sfu.ca
Date: Thu, 12 Oct 1995 07:29:45 -0700 (PDT)
Subject: Be Sample holder

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings!

We are looking for a new or used berylium double tilting sample holder for
JEOL JEM 2000FX TEM. Please let me know the price.

I greatly appreciate for your help.

Sandy Burany, PhD
Dept. of Physics and Astronomy
University of Delaware
Newark, DE 19716
(302) 831 3515




From: geosclmr-at-showme.missouri.edu (Lou Ross)
Date: Thu, 12 Oct 1995 09:04:43 -0600
Subject: TIFF convertors

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Message-Id: {199510121403.JAA98682-at-mail.missouri.edu}
X-Sender: geosclmr-at-pop.missouri.edu
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"


I've already sent Brendon a personal response about converting PC TIFF
images to MAC TIFF, but I thought I would post it to the group.

I've been using MacLinkPlus/PC Connect for several years to transfer IBM
TIFF images to a Mac though the serial line. It works great, I've never had
a problem. Included are all types of translators for both platforms and
desktop translators for each platform. (ie, Word to Wordperfect)

The company is Dataviz, 55 Corporate Dr., Trumbull CT, 06611 (800)
733-0030. It goes for ~$130 with annual updates for ~$40.

Hope this helps.
Lou Ross

101 Geological Sciences Bldg.
University of Missouri
Columbia, MO 65211
(314) 882-4777, 882=5458 fax






From: Davin Jutila :      DJUTILA-at-wpsmtp.siumed.edu
Date: Thu, 12 Oct 1995 15:48:48 -0600
Subject: Darkroom cloth

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Message-Id: {199510121323.GAA29466-at-holonet.net}

Hello all,
I'm new in this area of science and need some assistance:

We are in need of a 14'x11.5' section of darkroom cloth for seperating
one room with the ability to do light free analysis. We have ran into a
wall with our catalogs and resources in locating a vendor for such a
curtain. Could someone advise us on where we might find such a
vendor or suggest other solution that might have been done in the past.

Thank you in advance,
Davin

Davin B. Jutila
Flow Cytometry/Research Imaging Facility
Southern Illinois University School of Medicine
801 North Rutledge Mail Code - 1220
Springfield, IL 62794-9230
Ph#: (217)-782-0898 Fax#: (217)-524-3227
e-mail: djutila-at-wpsmtp.siumed.edu





From: PrueHeart2-at-aol.com
Date: Thu, 12 Oct 1995 17:33:37 -0400
Subject: Request for Media Kit

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sirs:

I am the publicist for VayTek, Inc. of Fairfield, IA.

Please send a media kit and editorial calendar for "Microscopy Research and
Technique" to:

Lynna Howard
VayTek Publicity Department
12375 North, 25 East
Idaho Falls, ID 83401
USA

Please also include a recent issue of your journal.

Thank you.




From: sfzane-at-unccvm.uncc.edu (Sandra F. Zane)
Date: Thu, 12 Oct 1995 14:17:37 -0400
Subject: Re: looking for well slides

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Does anyone out there know where a person might be able to locate at
} source for light microscopy 'well - slides'?
}
Richard,
Would hanging drop slides serve your purpose? If so Fisher has these.
Pp.1229 in their '95/'96 catalogue. They have 1 or 2 cavities in normal and
extra thick. Order no. 1-800-766-7000.
Hope this is helpful.

Sandra Zane
Sandra F. Zane, EM Tech.
Dept of Biol., UNC Charlotte
9201 University City Blvd.
Charlotte, NC 28223-0001
sfzane-at-unccvm.uncc.edu
Fax (704) 547-3128





From: PrueHeart2
Date: 95-10-12 17:33:46 EDT
Subject: Fwd: Request for Media Kit

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



---------------------
Forwarded message:
Subj: Request for Media Kit

Sirs:

I am the publicist for VayTek, Inc. of Fairfield, IA.

Please send a media kit and editorial calendar for "Microscopy Research and
Technique" to:

Lynna Howard
VayTek Publicity Department
12375 North, 25 East
Idaho Falls, ID 83401
USA

Please also include a recent issue of your journal.

Thank you.




From: lmiller-at-ux1.cso.uiuc.edu (Lou Ann Miller)
Date: Thu, 12 Oct 1995 13:45:30 -0600
Subject: Re: air filtration for microscopy lab

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Message-Id: {v01530503aca3205b80f2-at-[128.174.176.76]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Hi,

We use air purifiers in our darkrooms and it helps a lot} This is
especially true in the winter when in the past we have spent hours fighting
the dust battle to only find more dust on the neg when printing.

Being an asthmatic, and on their newsgroup, I've seen tons of postings on
the subject, and have researched it myself.

The best portable air purifier in most ( and my opinion) is the Honeywell
envirocare HEPA air purifier.
We have ionizers in our darkrooms, and they give off ozone, and snap a lot,
they also cost about $250 each. For Home I bought a Honeywell instead
after experiencing the ionizers.

The Honeywell HEPA is 99.97% effecient down to the 0.3 micron range, has a
low white noise that isn't bad, and smells really fresh. ie I'll stand by
my honeywell all day, but I don't want to get near those ionizers , yuck.

Cost: Depends upon model, 3 sizes that I know of, about $130-$225

Maintenance: Change charcoal prefilter once every 3 months ( pretty cheap)
Change main filter once every 2-5 years.- a little
more $$

Hope this helps some,

Lou Ann
--------------------------------------------------
Any others? Does anyone have sufficient experience with portable air
cleaners to make recommendations? Does anyone have a nifty design
for low-cost self-built bench clean air hoods?

Thanks.

James Martin

***********************
Lou Ann Miller
Microscopic Imaging Laboratory
College of Veterinary Medicine
University of Illinois
2001 S Lincoln Ave
Urbana, Illinois 61801
217-244-1566
lmiller-at-ux1.cso.uiuc.edu

Microscopy Lab:
http://www.cvm.uiuc.edu/announcements/MicSoc/MicImagLab.html

Personal Home Page:
http://www.cvm.uiuc.edu/HomePages/LouAnnMiller/Homepage.html
***********************






From: Marc Brande :      brande-at-sdsc.edu
Date: Thu, 12 Oct 1995 14:39:24 -0700 (PDT)
Subject: Physical 3D Cell Model Needed

Contents Retrieved from Microscopy Listserver Archives
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Neuroscience List {neur-sci-at-net.bio.net} ,
Morphmet List {morphmet-at-cunyvm.cuny.edu} ,
MolecularCellSpeak List {molecular-cell-speak-at-mailbase.ac.uk} ,
Microscopy List {microscopy-at-aaem.amc.anl.gov} ,
Confocal Microscopy List {confocal%ubvm.BITNET-at-BITNIC.CREN.NET} ,
Cell Bio List {cellbiol-at-net.bio.net} ,
Biz-Biotech List {biz-biotech-at-netcom.com} ,
Biomechanics List {biomch-l-at-nic.surfnet.nl} ,
Biomaterial List {biomat-l-at-hearn}
Message-Id: {Pine.3.89.9510121449.A28707-0100000-at-pauline.sdsc.edu}
Mime-Version: 1.0
Content-Type: TEXT/PLAIN; charset=US-ASCII

I am in need of a 3D physical model (plastic, etc.) of a cell for
demonstration. Any cell type will do. Does anyone know of manufacturer of
such an item for purchase?

Please mail me directly and not the list itself: (BRANDE-at-SDSC.EDU).
Thanks in advance for your help.

Marc

Marc C. Brande, M.S. SD3D Email List:3D Imaging
San Diego 3D Imaging Group To subscribe/unsubscribe:listserv-at-bobcat.etsu.edu
3840 Camino Lindo To post a message:sd3d-at-bobcat.etsu.edu
San Diego, CA 92122 Email:BRANDE-at-SDSC.EDU Voice:619-587-4830





From: bozzola-at-siu.edu (John. J. Bozzola)
Date: Thu, 12 Oct 1995 15:38:12 -0600
Subject: Re: Syquest-SCSI/DOS-MAC

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-Sender: bozzola-at-saluki-mail.fiber2.siu.edu
Message-Id: {v01510103aca33aaabf95-at-[131.230.97.77]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

I have been using AccessPC by Insignia Solutions in my Macs for several
years and have no trouble with DOS 44 MB SyQuests mounting each time,
flawlessly.



} I use Syquest removable media (44MB each) for image storage in the Mac and
} the PC. I purchased DOS MOUNTER from DAYNA Communication, inc and can mount
} easily floppy DOS-MAC. Are there any drivers out there (software?) that would
} allow me to bring a DOS formatted Syquest to the MAC without having to update
} the driver every time is inserted across plataforms? Thanks in advance.
}
} ******************************************************************
} *Cesar D. Fermin, Ph.D \|T|/ Fax (504) 587-7389 *
} *Tulane Medical School /|M|\ Voice Mail (504) 584-2618 *
} *Pathology/SL79 \|C|/ Secretary (504) 584-2436 *
} *New Orleans La 70112-2699 /|*|\ Lab/Techn. (504) 584-2521 *
} * {Fermin-at-tmc.tulane.edu} Dir. Morphological Services *
} ******************************************************************

#############################################################################
John J. Bozzola, Director
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
#############################################################################






From: PrueHeart2-at-aol.com
Date: Thu, 12 Oct 1995 17:53:08 -0400
Subject: Press Releases

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sirs:
If you prefer that future press releases be sent via snail mail, or to a
different email address, please let me know. Two press releases follow.
Lynna Howard

NEWS RELEASE
FOR IMMEDIATE RELEASE
September 25, 1995

New VoxBlastx Issued for Windows 95 and Windows NT

VayTek, Inc.'s software for rendering, measuring and presenting 3D data now
takes advantage of the Windows 95 and Windows NT operating systems.

The new Windows versions are significantly faster on a Pentium computer than
on the DX2 or DX4 computers. Its speed now rivals that of several of the UNIX
workstations.

VoxBlast is a cut above most 3D volume visualization programs, offering
powerful tools for extracting quantitative information. Arbitrary slicing
planes, true 3D measurements, surface extraction, polygon rendering, flood
fill capabilities, and volumetric calculations are just a few of the standard
features.

Unlike most other 3D rendering programs, VoxBlast measurements are based on
fractional interpolations and careful preservation of original data, so the
end results are more accurate.

The Windows versions of VoxBlast retain the high degree of flexibility and
compatibility of previous versions. Data sets from microscope data, CT scans,
petroleum engineering data, chip inspection, and other sources are readily
accepted, giving researchers a broad range of choices for ancillary
equipment.

Sophisticated lighting models, movie generation features, and palette editors
are included in the program. Presentaion and evaluation of data is enhanced
when the user incorporates color, transparency, motion, and lighting:
pseudocoloring is available from a 16 million-color palette; cutting planes
can be presented with one palette and opacity table on one side, and an
entirely different palette and opacity table on the other side; the
transparency feature will reveal details in the data set that would otherwise
be hidden. All of these features and many more can be used in self-scripted
movie loops.

Like its SGI-based equivalents, VoxBlast supports TIFF files, true 24 bit RBG
rendering, 16 bit dual mode rendering, and volume densitometry - but VoxBlast
runs on less expensive hardware.

VoxBlast is available on the Internet for evaluation. Contact VayTek at
515-472-2227 or vaytek-at-ins.infonet.net for download instructions.

xxx


NEWS RELEASE
FOR IMMEDIATE RELEASE
September 25, 1995

VoxBlastx Now Native for Power Mac

VayTek, Inc.'s software for rendering, measuring and presenting 3D data now
runs approximately three times faster in the new, native version for Power
Macs.

With this dramatic increase in speed, the Power Mac can match many UNIX
workstations in accepting a stack of registered 2D images for creation of 3D
projections.

VoxBlast is a cut above most 3D volume visualization programs, offering
powerful tools for extracting quantitative information. Arbitrary slicing
planes, true 3D measurements, surface extraction, polygon rendering, flood
fill capabilities, and volumetric calculations are just a few of the standard
features.

The native version of VoxBlast retains the high degree of flexibility and
compatibility of previous versions. Data sets from microscope data, CT scans,
petroleum engineering data, chip inspection, and other sources are readily
accepted, giving researchers a broad range of choices for ancillary
equipment.

Researchers at Pioneer Hi-bred in Iowa say, "For the price, Voxblast has no
equal." University of Missouri scientists add, "In addition to the high
quality images, we were impressed by the flexibility of VayTek's software."

Unlike most other 3D rendering programs, VoxBlast measurements are based on
fractional interpolations and careful preservation of original data, so the
end results are more accurate.

Sophisticated lighting models, movie generation features, and palette editors
are included in the program. Presentaion and evaluation of data is enhanced
when the user can incorporate color, transparency, motion, and lighting:
pseudocoloring is available from a 16 million-color palette; cutting planes
can be presented with one palette and opacity table on one side, and an
entirely different palette and opacity table on the other side; the
transparency feature will reveal details in the data set that would otherwise
be hidden. All of these features and many more can be used in self-scripted
movie loops.

Like its SGI-based equivalents, VoxBlast supports TIFF files, true 24 bit RBG
rendering, 16 bit dual mode rendering, and volume densitometry - but VoxBlast
runs on less expensive hardware.

VoxBlast is available on the Internet for evaluation. Contact VayTek at
515-472-2227 or vaytek-at-ins.infonet.net for download instructions.

xxx

Thank you for your time.






From: PrueHeart2
Date: 95-10-12 17:53:17 EDT
Subject: Fwd: Press Releases

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



---------------------
Forwarded message:
Subj: Press Releases

Sirs:
If you prefer that future press releases be sent via snail mail, or to a
different email address, please let me know. Two press releases follow.
Lynna Howard

NEWS RELEASE
FOR IMMEDIATE RELEASE
September 25, 1995

New VoxBlastx Issued for Windows 95 and Windows NT

VayTek, Inc.'s software for rendering, measuring and presenting 3D data now
takes advantage of the Windows 95 and Windows NT operating systems.

The new Windows versions are significantly faster on a Pentium computer than
on the DX2 or DX4 computers. Its speed now rivals that of several of the UNIX
workstations.

VoxBlast is a cut above most 3D volume visualization programs, offering
powerful tools for extracting quantitative information. Arbitrary slicing
planes, true 3D measurements, surface extraction, polygon rendering, flood
fill capabilities, and volumetric calculations are just a few of the standard
features.

Unlike most other 3D rendering programs, VoxBlast measurements are based on
fractional interpolations and careful preservation of original data, so the
end results are more accurate.

The Windows versions of VoxBlast retain the high degree of flexibility and
compatibility of previous versions. Data sets from microscope data, CT scans,
petroleum engineering data, chip inspection, and other sources are readily
accepted, giving researchers a broad range of choices for ancillary
equipment.

Sophisticated lighting models, movie generation features, and palette editors
are included in the program. Presentaion and evaluation of data is enhanced
when the user incorporates color, transparency, motion, and lighting:
pseudocoloring is available from a 16 million-color palette; cutting planes
can be presented with one palette and opacity table on one side, and an
entirely different palette and opacity table on the other side; the
transparency feature will reveal details in the data set that would otherwise
be hidden. All of these features and many more can be used in self-scripted
movie loops.

Like its SGI-based equivalents, VoxBlast supports TIFF files, true 24 bit RBG
rendering, 16 bit dual mode rendering, and volume densitometry - but VoxBlast
runs on less expensive hardware.

VoxBlast is available on the Internet for evaluation. Contact VayTek at
515-472-2227 or vaytek-at-ins.infonet.net for download instructions.

xxx


NEWS RELEASE
FOR IMMEDIATE RELEASE
September 25, 1995

VoxBlastx Now Native for Power Mac

VayTek, Inc.'s software for rendering, measuring and presenting 3D data now
runs approximately three times faster in the new, native version for Power
Macs.

With this dramatic increase in speed, the Power Mac can match many UNIX
workstations in accepting a stack of registered 2D images for creation of 3D
projections.

VoxBlast is a cut above most 3D volume visualization programs, offering
powerful tools for extracting quantitative information. Arbitrary slicing
planes, true 3D measurements, surface extraction, polygon rendering, flood
fill capabilities, and volumetric calculations are just a few of the standard
features.

The native version of VoxBlast retains the high degree of flexibility and
compatibility of previous versions. Data sets from microscope data, CT scans,
petroleum engineering data, chip inspection, and other sources are readily
accepted, giving researchers a broad range of choices for ancillary
equipment.

Researchers at Pioneer Hi-bred in Iowa say, "For the price, Voxblast has no
equal." University of Missouri scientists add, "In addition to the high
quality images, we were impressed by the flexibility of VayTek's software."

Unlike most other 3D rendering programs, VoxBlast measurements are based on
fractional interpolations and careful preservation of original data, so the
end results are more accurate.

Sophisticated lighting models, movie generation features, and palette editors
are included in the program. Presentaion and evaluation of data is enhanced
when the user can incorporate color, transparency, motion, and lighting:
pseudocoloring is available from a 16 million-color palette; cutting planes
can be presented with one palette and opacity table on one side, and an
entirely different palette and opacity table on the other side; the
transparency feature will reveal details in the data set that would otherwise
be hidden. All of these features and many more can be used in self-scripted
movie loops.

Like its SGI-based equivalents, VoxBlast supports TIFF files, true 24 bit RBG
rendering, 16 bit dual mode rendering, and volume densitometry - but VoxBlast
runs on less expensive hardware.

VoxBlast is available on the Internet for evaluation. Contact VayTek at
515-472-2227 or vaytek-at-ins.infonet.net for download instructions.

xxx

Thank you for your time.






From: mager-at-unixg.ubc.ca (Mary Mager)
Date: Thu, 12 Oct 1995 21:06:58 -0800
Subject: Re: X-ray Scanning Microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-Sender: mager-at-pop.unixg.ubc.ca
Message-Id: {v01510104aca3a0a0d33f-at-[137.82.220.131]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

To Jay Jerome,
The paper that resulted from the work in Calgary on converting an SEM to a
n X-ray microscope is:
"X-ray microscopy using a scanning electron microscope for the purpose of
imaging central nervous system structures" S. Fletcher, R.S. Hannah and
M.J. Hollenberg. Journal of Neuroscience Methods, 7 (1983) 19-25.
The microscope that was successfully converted was a Hitachi S-450 and the
resulting pictures were like conventional transmission X-rays, but at
magnifications of 20X to 2000X.

regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Eng, UBC
309 - 6350 Stores Rd.
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648, fax: 604-822-3619
email: mager-at-unixg.ubc.ca






From: mager-at-unixg.ubc.ca (Mary Mager)
Date: Thu, 12 Oct 1995 21:28:33 -0800
Subject: MAC, PC TIFF and Syquest

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-Sender: mager-at-pop.unixg.ubc.ca
Message-Id: {v01510106aca3a5ab028c-at-[137.82.220.119]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Dear All:
I understand that the later MACs will read PC-written floppies and programs
such as Photoshop and Graphics Converter will read the TIFFs on the PC
floppies, in the MAC. I know my Power MAC 6100 running System 7.5 will.
Also, we have a Syquest drive that normally resides on the PC, on a SCSI
port. That was the tough part. It was very simple to change the SCSI ID on
the back of the Syquest and plug it into the Power MAC 6100. The MAC found
it and labeled it "DOS" and accessed it fine.
Hope this helps.
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Eng, UBC
309 - 6350 Stores Rd.
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648, fax: 604-822-3619
email: mager-at-unixg.ubc.ca






From: ICleme1659-at-aol.com
Date: Fri, 13 Oct 1995 10:28:33 +0200
Subject: Flow Cytometry Standards phone/fax no

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Message-Id: {199510130828.JAA00505-at-pons.uio.no}
X-Sender: finnmog-at-pons.uio.no
X-Mailer: Windows Eudora Version 1.4.3
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Thanks for the answers!

*********************************************************
QUERY:
Their adress was cited by the Thomas register as
Flow Cytometry Standards Corporation
513 Hostos Avenue, Ste. B3
P.O. Box 194344
San Juan, Puerto Rico 00919
but TR could not find any phone/fax numbers.

ANSWERS:
809 753-9341 ( Abe Schwartz )



Finn-Mogens Haug

University of Oslo
Institute of basic medical sciences
Department of anatomy E-mail: f.m.haug-at-basalmed.uio.no
Box 1105 Blindern Phone : +47 22 85 12 67
N-0317 Oslo, NORWAY Fax : +47 22 85 12 78






From: hris-at-facstaff.wisc.edu (Hans Ris)
Date: Fri, 13 Oct 1995 05:18:43 -0500
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


unsubscribe- hris-at-facstaff.wisc.edu- Hans Ris Univ.Wisc.Madison





From: Michael Cammer :      cammer-at-aecom.yu.edu
Date: Fri, 13 Oct 1995 09:21:12 -0400 (EDT)
Subject: Re: looking for well slides

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Lab-Tek chambered coverglasses (for inverted microscopes) or chambered
slides made w/ glass or plastic. Call 1-800-288-NUNC.

Or SuperCell culture slides. Call 1-800-258-0834.

We have used the Nunc/Labtek products for years and like them a lot.
The SuperCell info is from an ad I noticed yesterday in BioTechniques.

-Michael Cammer

{null signature file}

On Thu, 12 Oct 1995, Richard E. Edelmann wrote:

} Does anyone out there know where a person might be able to locate at
} source for light microscopy 'well - slides'?
}
}




From: PrueHeart2-at-aol.com
Date: Fri, 13 Oct 1995 12:26:01 -0400
Subject: Apology

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


My apologies to everyone who subscribes to the list server. Two press
releases regarding VayTek products were inadvertently posted here.

My email address book for editors of technical and scientific journals
apparently contained the Microscopy list server. Sorry for any inconvenience.

This may seem incredibly stupid, but I didn't realize I had posted to the
list server.

Again, my apologies. Thank you to those of you who sent me polite and
informative notes.

Lynna Howard




From: MicroToday-at-aol.com
Date: Fri, 13 Oct 1995 13:25:27 -0400
Subject: New Server

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Nestor and Group -
I wonder if I, and perhaps others, may have missed some of the instructions
concerning the new server? Do I have it correct:
1) We are to "unsubscribe" from the old server and, I trust, that we all
know how to do this.
2) We are then to "subscribe" to the new server?
3) To "subscribe" to the new server, we should follow the rules from the old
server - to wit: Send a message to "Listserver-at-MSA.microscopy.com" and then
in the text block to do a "subscribe" followed by ones' eMail address.
I wonder (and hope) that the above is correct?
Regards to all
Don Grimes





From: Doug Cromey :      dcromey-at-ccit.arizona.edu
Date: Fri, 13 Oct 1995 11:08:15 -0700
Subject: ? re: dye for locating blood vessels

Contents Retrieved from Microscopy Listserver Archives
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Dear Microscopists,
One of the people in our department asked me if I knew of a dye that they
could use to locate the vena cava in rats. They want to disect the vena
cava and the aorta and ultimately do EM on the tissue, so the dye can't be
particularly nasty. They have been having trouble locating the vena cava.
Any ideas out there?

Thaks,
Doug
.....................................................................
: Douglas W. Cromey, M.S. Dept. of Cell Biology & Anatomy :
: Sr. Research Specialist University of Arizona :
: (office: AHSC 4212A) P.O. Box 245044 :
: (voice: 520-626-2824) Tucson, AZ 85724-5044 USA :
: (FAX: 520-626-2097) (email: dcromey-at-ccit.arizona.edu) :
:...................................................................:





From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Fri, 13 Oct 1995 17:07:45 -0500
Subject: LKB Nova trimming block

Contents Retrieved from Microscopy Listserver Archives
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Message-Id: {199510132209.RAA03724-at-bcm.tmc.edu}
X-Sender: joiner-at-bcm.tmc.edu
X-Mailer: Windows Eudora Version 2.1.1
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Dear Microscopists (or maybe more appropriately: Microtomists) -

I have just inherited a used LKB Nova ultramicrotome. However it is missing
block holders for 8 mm Beem blocks and the trimming block. If anyone of you
have either of these items cluttering up your lab, and you no longer need
them or don't want them, I would be very happy to relieve your burden. We
are mainly in need of the trimming block. Make me an offer I can't refuse!


Joiner Cartwright, Jr., Ph.D.
Director, Electron Microscopy
Department of Pathology, Rm.286-A
Baylor College of Medicine
One Baylor Plaza
Houston, Texas 77030 U.S.A.
tel.: (713)798-4658
FAX: (713)798-3945
joiner-at-bcm.tmc.edu
Compuserve: 71555,1206





From: MicroToday-at-aol.com
Date: Sat, 14 Oct 1995 08:51:16 -0400
Subject: More on new listserver

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello again, Nestor and group -
Thanks to several for their comments re: my last note.
I do understand, thanks to Nestor, that one does not have to sign off the old
server and sign on the new server - and that the old system will transfer to
the new. It seemed, however, that Nestor is recommending that we do so?
My question might better be, if one wishes to subscribe (or unsubscribe) on
the new server, does he/she follow the old server system and address the
requests to "Listserver-at-msa.microscopy.com" - followed by "subscribe" (or
unsubscribe), and ones email address?
This seems logical, challenged only by the number of folks who are attempting
to subscribe, etc. directly on the new server.
Sorry to clutter the system - but hope that others, like me, are not sure of
the new system.
Regards to all,
Don Grimes





From: Donna Wagahoff :      DWAGAHOF-at-wpsmtp.siumed.edu
Date: Sun, 15 Oct 1995 11:00:52 -0600
Subject: cost recovery poll

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


** Reply Requested by 10/16/1995 (Monday) **

The budget office has asked us to evaluate the charges in our core
facility and we would like to compare them with those of other facilities.
Currently, we charge for using the TEM, SEM and Image Analysis
Systems and we charge for our technical assistance with particular
parts of the experimental procedure(e.g. sectioning). However, the use
of the ultramicrotomes, the sputter coater and the CPD are free.
So that we might provide our budget office with comparative
information, please share with us the charges used in your facility and
the cost recovery achieved by those charges. (In our lab the
microscopes and technical assistance charges are $15 per hour and the
image analysis charges are $6 per hour.)
Thanks for your assistance!

Donna Wagahoff
SIU School of Medicine
P.O. Box 19230
Springfield, Il. 62794-1220
217-782-0898
FAX 217-524-3227





From: zaluzec-at-Microscopy.Com (Nestor J. Zaluzec)
Date: Sun, 15 Oct 1995 15:09:07 -0500
Subject: Good Times Virus is a Hoax Ignore it....

Contents Retrieved from Microscopy Listserver Archives
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Subscribers.....

For those few of you that haven't yet heard about it. The good
times virus report is a hoax. Ignore it and DO NOT forward it to
anyone. A version of that message has been circulating the
Internet for nearly two years, with some slight modifications..
It amounts to a chain letter of sorts, just using up bandwidth, and
it's only virus capablities are that it continues to resurface in
email boxes when a new user finds the message and forwards
it around to his/her friends.

Nestor
Your Friendly Neighborhood SysOp






From: EvexAnalyt-at-aol.com
Date: Sun, 15 Oct 1995 18:21:26 -0400
Subject: unsubscribe

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unsubscribe




From: hris-at-facstaff.wisc.edu (Hans Ris)
Date: Sun, 15 Oct 1995 16:58:48 -0500
Subject: unsubscribe

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unsubscribe microscopy hrisfacstaff.wisc.edu Hans Ris





From: hris-at-facstaff.wisc.edu (Hans Ris)
Date: Sun, 15 Oct 1995 17:06:14 -0500
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
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unsubscribe Microscopy hris-at-facstaff.wisc.edu Hans Ris





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-aaem.amc.anl.gov
Date: Sun, 15 Oct 1995 23:06:12 -0500 (CDT)
Subject: Clarification from Nestor

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Don et al

Let me clarify, or at least attempt to do that.

You should now send all "Microscopy" mail to the address

Microscopy-at-MSA.Microscopy.Com

You should now send all Subscribe/Unsubscribe mail to the address

Listserver-at-MSA.Microscopy.Com


when you reply to a comment and you wish to allow all subscribers
of the Microscopy Listserver to see your reply you should
SEND a NEW MAIL message to

Microscopy-at-MSA.Microscopy.Com

if you use the "reply" function of most Email programs your
reply will go only to the originator of the message and not
to the entire list.


Many people have aliases, shortcuts, nicknames or some other
term defined in their Email client programs which allow them
to type a short abbreviated phrase to send Email to Microscopy.
My request is that all of you change your "nickname" files to
use the new address of the listserver.

As a transition from the old site to the new site, I have put
an automatic forwarding function on the old site. However, I
cannot guarentee that it will be there forever. I did this for
2 reasons, 1.) Many new subscribers find out about the list
server from old publications, this allows them to still access
the system, in their instructions I will remind them of the
new address 2.) Lots of you will forget to change your aliases
and then I'll get bunches of mail asking what is wrong if the
old address immediately disappears. So it is also my attempt at
making my life a bit simplier, however, I never seem to be
able to succeed at actually doing that.



Later... Nestor

Your Friendly Neighborhood SysOp






From: t.bostrom-at-qut.edu.au (Thor Bostrom)
Date: Mon, 16 Oct 1995 17:59:09 +1000
Subject: Re: TIFF FILE CONVERTER FOR PC

Contents Retrieved from Microscopy Listserver Archives
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} WE are having problems crossing the MAC IBM barrier with TIFF images. Does
} anyone know of a simple utility for IBM to convert an IBM-TIFF image file
} to otehr formats, MAC readable TIFF at least.
} Many thanks
} Brendon J. Griffin
} Centre for Microscopy and Microanalysis
} The University of Western Australia
} Nedlands, WA, AUSTRALIA 6907
} ph 61-9-380-2739 fax 61-9-380-1087
}

We also occasionally need to move TIFF files from eg. our Leica TCS4D
confocal via a PC to an image analysis package on a Mac Quadra running
System 7.1. We use PC Exchange on the Mac to read and write PC format disks,
and import TIFF files with Adobe Photoshop, which reads (& writes) both
Intel and Motorola (Mac) TIFF formats. The image can then be resaved as a
PICT file. Alternatively one can use Apple File Exchange to import TIFF
files, but it's slow.

For the PC, to add to the software already mentioned, there's a program
called Image Alchemy (for DOS) available as shareware, which converts a huge
variety of graphics formats, including Intel and Motorola TIFF. It can
convert a PC TIFF file to Macintosh PICT format, which can be read directly
into a Mac. The only complication is that the Mac file type should be set to
PICT, so that it is recognized as such. With PC Exchange, you can configure
it so that a file with the appropriate PC file extension (eg .PCT) is
recognized as a PICT file.

Hope it helps. Regards,

Thor Bostrom
Analytical EM Facility, QUT
Brisbane, Australia







From: Andreas Haug :      ajh-at-ipc.uni-tuebingen.de
Date: Mon, 16 Oct 1995 14:52:04 +0100 (BST)
Subject: TEST: new aliases

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Message-Id: {199510160932.KAA07024-at-atlas.ipc.uni-tuebingen.de}
Content-Type: text/plain
MIME-Version: 1.0 (NeXT Mail 3.3 v118.2)

I go away for a week and three discussion groups change address!!
--
Dr. Keith R. Hallam University of Bristol, Interface Analysis Centre, Oldbury
House, 121, St. Michael's Hill, Bristol, BS2 8BS, England
Telephone: + 44 (0)117 925 5666 | E-mail: k.r.hallam-at-bristol.ac.uk
Facsimile: + 44 (0)117 925 5646 | URL: http://zeus.bris.ac.uk/~phkrh/




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-aaem.amc.anl.gov
Date: Sun, 15 Oct 1995 23:06:12 -0500 (CDT)
Subject: Clarification from Nestor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Folks....

I must apologize. There was a major typo on the clarification
I sent out late last night. Blame it on just my being tired. All
subscribe/unsubscribe messages should go to

Listserver-at-MSA.Microscopy.Com

I've corrected the text below.

Nestor
-------------------------


Let me clarify, or at least attempt to do that.

You should now send all "Microscopy" mail to the address

Microscopy-at-MSA.Microscopy.Com

You should now send all Subscribe/Unsubscribe mail to the address

Listserver-at-MSA.Microscopy.Com

when you reply to a comment and you wish to allow all subscribers
of the Microscopy Listserver to see your reply you should
SEND a NEW MAIL message to

Microscopy-at-MSA.Microscopy.Com

if you use the "reply" function of most Email programs your
reply will go only to the originator of the message and not
to the entire list.


Many people have aliases, shortcuts, nicknames or some other
term defined in their Email client programs which allow them
to type a short abbreviated phrase to send Email to Microscopy.
My request is that all of you change your "nickname" files to
use the new address of the listserver.

As a transition from the old site to the new site, I have put
an automatic forwarding function on the old site. However, I
cannot guarentee that it will be there forever. I did this for
2 reasons, 1.) Many new subscribers find out about the list
server from old publications, this allows them to still access
the system, in their instructions I will remind them of the
new address 2.) Lots of you will forget to change your aliases
and then I'll get bunches of mail asking what is wrong if the
old address immediately disappears. So it is also my attempt at
making my life a bit simplier, however, I never seem to be
able to succeed at actually doing that.



Later... Nestor

Your Friendly Neighborhood SysOp



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From: ldm2-at-apollo.numis.nwu.edu (L.D.Marks)
Date: Mon, 16 Oct 1995 12:25:34 -0500
Subject: CCD's for TEM's

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Message-ID: {D569E82F01681600-at-c2gate.tcom.co.uk}

I (as presumably did others) have just received Princeton
Instrument's brochure in the mail. My understanding after talking
to them at MSA is that their systems are considerably cheaper
than Gatan's, particularly at the 2048x2048 size.

Any comments, pieces of wisdom, experience with using these
systems?

Laurie Marks




From: Tom Taylor :      ttaylor-at-msai.mea.com
Date: Mon, 16 Oct 1995 16:34:26 -0400
Subject: unsubscribe

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--
Regards,

Tom Taylor {ttaylor-at-msai.mea.com}




From: Daniel L. Callahan :      dlc-at-owlnet.rice.edu
Date: Mon, 16 Oct 1995 11:56:57 -0500 (CDT)
Subject: textbooks for physical CTEM

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Hello:

I think I keep a pretty good eye on microscopy literature but thought
that it would be a good idea to poll the community at large.

What textbooks are being used to teach TEM, particularly at the introductory
graduate level for physical scientists? I would like to restrict this
line to books currently in print ;)

I have been very impressed by two collaborative texts in SEM and AEM:
Goldstein et al's Scanning Electron Microscopy and X-Ray Analysis and
Joy et al's Principles of Analytical Electron Microscopy. These books
both seem well-written, particularly for collaborative projects, and
suitable both for class-use and excellent long-term references. However,
I don't know of any book for conventional TEM in print which I regard as
highly. Incidentally, Principles of AEM does provide a fine introduction
to CTEM but its heart is clearly in the explanation and use of
spectroscopy and microdiffraction.

I recall being relatively pleased with a recent book written specifically
for mineralogists; someone else must have liked it too since it has
walked off my shelf. I would really prefer a more general text for
physical scientists though, since I deal with substantial numbers of
materials scientists, chemists, and EE's.

Thoughts?

Daniel L. Callahan
Department of Mechanical Engg. and Materials Science
Rice University
dlc-at-owlnet.rice.edu




From: tphillips-at-biosci.mbp.missouri.edu (Tom Phillips)
Date: Mon, 16 Oct 1995 11:41:56 -0600
Subject: EM faculty opening

Contents Retrieved from Microscopy Listserver Archives
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TENURE TRACK FACULTY POSITION
University of Missouri - Columbia

The University of Missouri-Columbia (MU) seeks a tenure-track Assistant,
Associate, or full Professor to be jointly appointed in the colleges of
Veterinary Medicine and Agriculture, Food, and Natural Resources. The
successful candidate will be expected to lead an outstanding independent
research program in an area of cell or structural biology of importance to
basic biomedical aspects of veterinary medicine and agriculture, teach
electron microscopy at the graduate level, and direct (at 20% effort) a
recently established, campus-wide Electron Microscopy Core Facility under
the auspices of the MU Molecular Biology Program. Experience in both plant
and animal systems is preferred, but not required; post-doctoral experience
is necessary. Women and members of minority groups are especially
encouraged to apply. Applicants should submit, by January 31, 1996, a
description of research plans, curriculum vitae, selected reprints and
names, e-mail addresses, and telephone/FAX numbers of three professional
references to: Dr. Margaret A. Miller, co-chair, Cell Ultrastructure
Faculty Search Committee, UMC Veterinary Medical Diagnostic Laboratory, PO
Box 6023, Columbia, MO 65205; phone, 314/882-6811; FAX, 314-884-7544;
e-mail: miller-at-vmdl.missouri.edu. The University of Missouri is an
Affirmative Action/Equal Opportunity Employer.




Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility
3 Tucker Hall
University of Missouri
Columbia, MO 65211
(314)-882-4712 (voice)
(314)-882-0123 (fax)






From: Laurie Frederick :      frederick_laurie-at-VANLAB.PAPRICAN.CA
Date: Mon, 16 Oct 1995 11:00:04 PDT
Subject: Re: Darkroom cloth

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Message-Id: {MAILQUEUE-101.951016110004.416-at-vanlab.paprican.ca}
To: Davin Jutila {DJUTILA-at-wpsmtp.siumed.edu}

Hi,
I have worked in a couple of labs with similar curtains made out of a
regular heavy dark fabric, but if I was to do it myself, I would look at
the blackout liners available for drapes. Enquire at any local drapery
retailer. The reinforced vinyl fabric has the combined advantages of
being opaque and non lint producing.
Laurie

} Hello all,
} I'm new in this area of science and need some assistance:
}
} We are in need of a 14'x11.5' section of darkroom cloth for seperating
} one room with the ability to do light free analysis. We have ran into a
} wall with our catalogs and resources in locating a vendor for such a
} curtain. Could someone advise us on where we might find such a
} vendor or suggest other solution that might have been done in the
past.
}
} Thank you in advance,
} Davin
}
} Davin B. Jutila
} Flow Cytometry/Research Imaging Facility
} Southern Illinois University School of Medicine
} 801 North Rutledge Mail Code - 1220
} Springfield, IL 62794-9230
} Ph#: (217)-782-0898 Fax#: (217)-524-3227
} e-mail: djutila-at-wpsmtp.siumed.edu
}
}

______________________________________________________________________
Laurie Frederick, A.SC.T. PAPRICAN
Corrosion Control Group 3800 Wesbrook Mall
The Pulp and Paper Research Vancouver, B.C.
Institute of Canada Canada V6S 2L9

Email: frederick_laurie-at-vanlab.paprican.ca
Tel: 604-222-3200 Fax: 604-222-3207




From: Po-Fu Huang :      huang020-at-maroon.tc.umn.edu
Date: Mon, 16 Oct 1995 10:41:55 -0500 (CDT)
Subject: Apology for "Good Times"

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Dear friends,

I apologize for the message I forwarded to you few days ago regarding to
the virus "Good Times". Thanks for all who inform me the truth. I have
forwarded Mr. Haug's condensed version of the "Good Times FAQ" to whom I
received the mail from. Hopefully, this joke chain letter won't be spread
any further.

Best regrads,

Po-Fu Huang

Particle Technology Laboratory (Office) 612-626-7227
Mechanical Engineering Department (Lab) 612-625-7307
University of Minnesota (e-mail) huang020-at-maroon.tc.umn.edu

The LORD is my shepherd. I shall not be in want. -- Psalm 23:1






From: wesaia-at-iastate.edu (Warren Straszheim)
Date: Mon, 16 Oct 1995 13:44:40 -0500
Subject: just testing the address

Contents Retrieved from Microscopy Listserver Archives
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I have been having troubles sending mail to the new address. This is trying
the SPARC5 form.
----------------------------------------------------
Warren E. Straszheim
270 MD Ames Lab/ISU, Ames IA, 50011
Phone: 515-294-8187 FAX: 515-294-3091
E-Mail: wes-at-ameslab.gov (or: wesaia-at-iastate.edu)

coal characterization and processing
electron microscopy, x-ray analysis, image analysis
computer applications





From: wise-at-VAXA.CIS.UWOSH.EDU
Date: Mon, 16 Oct 1995 12:45:19 +0000
Subject: Re: LKB Nova trimming block

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Dear Microscopists (or maybe more appropriately: Microtomists) -
}
} I have just inherited a used LKB Nova ultramicrotome. However it is missing
} block holders for 8 mm Beem blocks and the trimming block. If anyone of you
} have either of these items cluttering up your lab, and you no longer need
} them or don't want them, I would be very happy to relieve your burden. We
} are mainly in need of the trimming block. Make me an offer I can't refuse!
}
}
} Joiner Cartwright, Jr., Ph.D.
} Director, Electron Microscopy
} Department of Pathology, Rm.286-A
} Baylor College of Medicine
} One Baylor Plaza
} Houston, Texas 77030 U.S.A.
} tel.: (713)798-4658
} FAX: (713)798-3945
} joiner-at-bcm.tmc.edu
} Compuserve: 71555,1206


I, too, inheirited an LKB 7800 without trimming blocks. So I made one
composed of a base (8" by 10" piece of 1" thick plexiglas; drilled and
countersunk (from bottom) for bolt), a 2.5" bolt, an ~1.25" spacer made out
of metal electrical conduit, a 1/2" drill chuck,m and a couple of big
washers. Pass the bolt up through the bottom of the base, add washer, slip
the 2 1/2" spacer over the bolt, another washer, then thread the chuck down
on the bolt to lock the whole gizmo in place. This setup has full 360o
rotation but no tilt. This has not been a problem.

I took the glass base plate out of a dissecting microscope and set the
scope over the top of the vertically-pointing drill chuck. Hand-tightening
of the drill chuck works fine.

The whole set-up cost about $25--so I made three of them.

Let me know if you need more specific plans. I can draw them up.







From: wesaia-at-iastate.edu (Warren Straszheim)
Date: Mon, 16 Oct 1995 13:44:01 -0500
Subject: just testing the address

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have been having troubles sending mail to the new address. This is trying
the MAS alias form.
----------------------------------------------------
Warren E. Straszheim
270 MD Ames Lab/ISU, Ames IA, 50011
Phone: 515-294-8187 FAX: 515-294-3091
E-Mail: wes-at-ameslab.gov (or: wesaia-at-iastate.edu)

coal characterization and processing
electron microscopy, x-ray analysis, image analysis
computer applications





From: philippe.buffat-at-cime.uhd.epfl.ch (Philippe-Andre Buffat)
Date: Tue, 17 Oct 1995 10:41:19 +0100
Subject: Adresse MSA

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X-Sender: pabuffat-at-cimedec1.epfl.ch
Message-Id: {aca92a56010210041daf-at-[128.178.98.14]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

************************************************************
Welcome to
************************************************************

Microscopy-at-MSA.Microscopy.Com

************************************************************
Hosted and Sponsored by The Microscopy Society of America (MSA)
as a Free Service to the World-Wide Microscopy & Microanalysis Community
************************************************************


G'day Subscribers....... (Well at least I hope it is day-time, when you
read this )

It looks as if we are now back on-line, and as the subject title and the above
banner indicate some major changes have occurred during the last few days.
You might even say we now have a new face, well at least a new envelope !

Firstly, as most of you know I had been running the Microscopy Listserver
at ANL for just over 2 years, basically without any real support, save my
time and
some electricity. As a consequence it was becoming harder and harder to keep
things running properly, let alone efficiently.

At this time it is my pleasure to officially announce that the Microscopy
Listserver has
moved (hopefully, nearly transparently to you but obviously with a bit of
work for me)
to a new address and with a new sponsor.

MSA at their 1995 Winter/Spring Council meeting, voted to establish a full
internet site
for the society and it's members and to host the Microscopy Listserver as a
FREE service to the microscopy and microanalysis community worldwide. It
has taken some time to get everything running, but as of last night, the
listserver is now relocated and functioning with a modicum of success. I
still basically do the majority of the work (gratis), however, we now have
a new
workstation (Sun Sparc5), software, and as I mentioned an offical internet
site.
The server will still need some work, but we now have the resources to
improve the system and make it more user-friendly.

For example, with the new configuration of header (i.e. the envelope of
this Email message), errors and undeliverables should now no-longer be
returned to the originator, but are sent to the SysOp
( Ijust need more mail, right?). In addition, I will be bringing up
on-line some of the more usual features characteristic of listserver
software in the coming weeks . I will post the information to to the list
as the options become functional.


I would encourage you to send thanks to MSA Council for saving the
listserver from
a slow death, as the hardware and software configurations at ANL were
becoming increasing difficult to maintain and as some of you know were
beginning to get particuliarly ornery.
} From my own perspective, my thanks go to all the members of council for
establishing
this site, and a special thanks to two members who have been especially
supportive
namely- Ron Gronsky of UC Berkeley and Ron Anderson of IBM.

The general MSA Email Address is:

MSA-at-MSA.Microscopy.Com

while the business office can be reached at:

MSABusinessOffice-at-MSA.Microscopy.Com.

Additional information about MSA, the society and it's operations can be
found at the MSA WWW site:

http://WWW.MSA.Microscopy.Com

MSA also mirrors the MMSLIB (Microscopy & Microanalysis Software Library)
anonymous ftp site at:

FTP.MSA.Microscopy.Com

and as you already know the Microscopy Listserver at:

Microscopy-at-MSA.Microscopy.Com

Mail from the old server site (Microscopy-at-AAEM.AMC.ANL.GOV) will be
automatically
forwarded to the new server site for awhile, but please change your local
aliases and/or
shortcuts, so that your mail goes directly to the MSA site instead of
channeling through ANL.

As I mentioned, there will still be a few growing pains, but the hard part
should be basically done.

------------------------------------------------------------------------------

In addition to the move from the ANL to the MSA site, the listserver has
passed another milestone this weekend, the 3,000th subscription request
arrived, not bad for only 2 years of operation. The lucky winner of a beer
should he and I ever meet is:

Russell J. Wilson
Analytical Electron Microscope Facility
Queensland University of Technology
Garden Point
G.P.O Box 2434
Brisbane Qld. 4001.

Russell, it's your job to make sure that a cool slab of tinney's is
around, but I'll buy!

--------------------------------------------------------------------------------

BTW if you are a triva buff or just curious the list sends Email to
Microscopists in
39 countries around the world. Over 5600 Email messages have been
submitted
to Microscopy over the period Oct 1, 1993 to Oct 1, 1995. Not all
of them have
been gems, but there have been occasional useful bits of info......

----------------------------------------------------------------------------
-----


...... Nestor

Your Friendly Neighborhood SysOp

__________________________________________________________________
Philippe Buffat Ecole Polytechnique Federale de
Lausanne (EPFL)
Centre Interdepartemental
de Microscopie Electronique
Address: EPFL-CIME, Batiment MX-C, CH-1015 Lausanne, Switzerland
Phone: +41(21)693 29 83 Fax: +41(21)693 44 01 (Central European Time)
E-mail: philippe.buffat-at-cime.uhd.epfl.ch // pabuffat-at-cimesg1.epfl.ch
______________________________ Eudora F2.1 ___________________________






From: Robert McDonald :      robert-at-geology.gla.ac.uk
Date: Tue, 17 Oct 1995 13:44:03 +0100
Subject: EPMA powder sample prep

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Message-Id: {16424.199510171244-at-starav.geology.gla.ac.uk}


I have been asked to find out how to prepare powder
samples for microprobe alaysis and I wondered if anyone
out there in Microland did this on a routine basis.

Any pointers would be most helpful.

I have had previous samples made into pellets using a
press which was used to prepare IR spectroscopy discs
but the results are rather disappointingly bumpy.

Thanks in advance,

Robert McDonald & Cameca SX50
Dept of Geology & Applied Geology
Glasgow University
Glasgow
SCotland

robert-at-geology.gla.ac.uk




From: wesaia-at-iastate.edu (Warren Straszheim)
Date: Tue, 17 Oct 1995 09:16:18 -0500
Subject: Re: just testing the address

Contents Retrieved from Microscopy Listserver Archives
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} I have been having troubles sending mail to the new address. This is trying
} the MAS alias form.
----
Sorry for the inconvenience my two posting may have caused. Nestor picked up
on the subtle problem. I had been sending my mail to MAS.Microscopy.Com as
hinted above. Strange enough, I got the address right in the header of my
test message even though I referred to _MAS_ in the body. Thanks to Nestor
for pointing out my dyslexia. I checked through my past attempts to post and
saw that the MAS error was indeed the culprit. It just took more than a
week to figure that out.

By the way, I never found the typo that Nestor fixed the other day in his
Typo in Clarification message. But all is well now, right?
----------------------------------------------------
Warren E. Straszheim
270 MD Ames Lab/ISU, Ames IA, 50011
Phone: 515-294-8187 FAX: 515-294-3091
E-Mail: wes-at-ameslab.gov (or: wesaia-at-iastate.edu)

coal characterization and processing
electron microscopy, x-ray analysis, image analysis
computer applications





From: David.Shortt-at-is.dal.ca (David Shortt)
Date: Tue, 17 Oct 1995 13:00:06 -0300
Subject: SEM Looking for parts.

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Am looking for parts for an Amray AMR1000 and a Cambridge S150.





From: roberts-at-CSSS.la.asu.edu (Bob Roberts)
Date: Tue, 17 Oct 1995 10:39:14 -0700
Subject: Two Items for Sale

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Hello Listerver Subscribers;

We are reorganizing our sample preparation lab and in the interest of
eliminating duplicative equipment/capabilities, we have two items to offer
for sale. The first is a dimpler/grinder (for 3mm samples) including
accessories. Secondly, we have an ultrasonic 3mm disc cutter. Both
instruments are in nearly new condition and the first reasonable offer
received will be accepted. You may contact me, or preferrably, Dr. Farhad
Shaapur, Center for Solid State Science, (602) 965-0399, or
shaapur-at-csss.la.asu.edu for further specifics.







From: xxie-at-lsuvm.sncc.lsu.edu (Xiaogang Xie)
Date: Tue, 17 Oct 1995 12:48:52 -0600
Subject: Re: EPMA powder sample

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Hi, Robert,

I do not have experience in analyzing powder samples, here are just
some technical thoughts (assume you are considering WDS detectors). A few
things should be considered in EPMA analysis:

1. the sample has to stand for the electron beam without any
physical and chemical change during analysis.
2. the sample need an ideal geometry, a smooth flatten surface in
most cases, for matrix and other corrections in analyzing.
3. for the same reason you also need standards that have similar
structure and composition as the sample.
I think the IR spectroscopy disc-type sample is not strong enough
to hold the particles during the analysis. (Also there are better ways to
analyze powder samples, such as XRF, ICP-AES(or MS), AA, and wet chemical
method. The point to use EPMA is for analyzing the micro scale phases or
particles that are difficult to be extracted from the whole sample).

Xiaogang

****************************************
* Xiaogang Xie *
* Department of Geology and Geophysics *
* Louisiana State University *
* Baton Rouge, LA 70803 *
* Office (504)388-2240 *
* Fax (504)388-2302 *
****************************************






From: bozzola-at-siu.edu (John. J. Bozzola)
Date: Tue, 17 Oct 1995 14:39:33 -0600
Subject: CSMS FALL MTG ST. LOUIS

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COMBINED CENTRAL STATES MICROSCOPY SOCIETY AND MISSOURI-ILLINOIS-KANSAS
MICROBEAM ANALYSIS SOCIETY MEETING

WHEN: ON NOVEMBER 10, 1995

WHERE: JOE HANON'S RESTAURANT, 2430 Old Dorsett Drive (just off of I-270
and Dorsett exit) IN ST LOUIS, MO..

SPEAKERS: Jon McCarthy (MIKMAS Sponsored Speaker) will discuss recent
advances in the area of EDS. CSMS Sponsored Spears include: Nestor Zaluzec
(that's right, the one and only) who will talk about telepresence
microscopy and recent advances in digital communication of microscopical
images. Susan Wente (Washington University, St. Louis) will present a
discussion of epitope labeling, a new technique for molecular localization
in the biological sciences.

TIME: MIKMAS will have the morning slot and CSMS the afternoon slot.

MORE INFORMATION: Detailed maps and a more developed program will be mailed
out in one week to interested parties.

CONTACT: John Bozzola at above e-mail address.


#############################################################################
John J. Bozzola, Director
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
#############################################################################






From: albrite-at-netcom.com (larry)
Date: Tue, 17 Oct 1995 14:16:28 -0700
Subject: WTB Film back for microscope camera.

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I am using a Nikon Microflex UFX camera setup on a light microscope with
Hoffman modulation. I have a 4x5 back and am wanting to purchace a 35mm
"Dark Box" FX 35-A or FX35-WA. These are obsolete and I have struck out
with the ususal sources. Anyone with one for sale respond directly to me.
Larry Albright
419 Sunset Avenue
Venice,CA 90291
Phone 310-399-0865
albrite-at-netcom.com
albrite-at-leonardo.net.
Thanks! Larry Albright




From: albrite-at-leonardo.net (Larry Albright)
Date: Tue, 17 Oct 1995 12:18:00 -0800
Subject: WTB Microscope 35mm back.

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I am using a Nikon UFX electronic camera on a Light microscope. I only have
the 4X5 back and am looking for a film holding back for 35mm work.
The model is FX35-A or FX35-WA, either will work with my system.
Anyone with one to sell please contact me direct. Any help will be
appreciated. Larry Albright


_--_ _--_
/#()# #\ 0 0 /# #()#\
|()## \#\_ \ / _/#/ ##()|
|#()##-=###\_ \ / _/###=-##()#|
\#()#-=## #\_ \ / _/# ##=-#()#/
|#()#--==### \_ \ / _/ ###==--#()#|
|#()##--=# #\_ \!!!/ _/# #=--##()#|
\#()##---===####\ O|O /####===---##()#/
|#()#____==#####\ / Y \ /#####==____#()#|
\###______######|\/#\/|######______###/
Larry Albright ()#O#/ ##\_#_/## \#O#() albrite-at-netcom.com
310=399-0865 ()#O#(__-===###/ _ \###===-__)#O#() albrite-at-leonardo.net
()#O#( # ###_(_|_)_### # )#O#()
()#O(---#__###/ (_|_) \###__#---)O#()
()#O#( / / ##/ (_|_) \## \ \ )#O#()
()##O#\_/ #/ (_|_) \# \_/#O##()
\)##OO#\ -) (_|_) (- /#OO##(/
)//##OOO*| / | \ |*OOO##\\(
|/_####_/ ( /X\ ) \_####_\|
/X/ \__/ \___/ \__/ \X\
(#/ \#)








From: mager-at-unixg.ubc.ca (Mary Mager)
Date: Tue, 17 Oct 1995 18:33:04 -0800
Subject: Re:EPMA analysis of powders

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Message-ID: {n1398158462.91502-at-mse.engin.umich.edu}

Dear Robert:
I have prepared powder samples for the EPMA many times, but have never
found a truly acceptable way. Usually I mix the powder with epoxy and make
a one inch button, then polish and carbon-coat it. This is OK for larger
particles and dense materials, but particles smaller than the
beam-interaction volume will give inaccurate quant. results. If you can
achieve good density with your press, use the lowest magnification
compatible with your spectrometers to smooth out surface roughness effects.
Preparing a set of well-characterized standards by the same method will
help.

Best of luck,
Mary


Mary Mager
Electron Microscopist
Metals and Materials Eng, UBC
309 - 6350 Stores Rd.
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648, fax: 604-822-3619
email: mager-at-unixg.ubc.ca






From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Wed, 18 Oct 1995 17:42:33 GMT+1200
Subject: Silicone Oil in Diffusion Pumps of Carbon Coaters

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Thanks to all who replied to my original query of October 10, it
seems that the overwhelming consensus is that if one wants to avoid
Si contamination of specimens (as I do, not all minerals contain Si),
one should avoid silicones. Most people seem to use Santovac 5.
So on to the inevitable next question, which is:
How the heck can I clean out the system in preparation for
changing to Santovac?
One user suggested heating it (off the system) with dishwash
detergent solution, but I'm pessimistic about the chances of this
removing silicone oil and sludge. I've heard a rumour of a suitable
solvent, but no details.

Has anybody got a good way?

I have to have my battle plan ready before I take the coater out of
service, as I have only one coater, can't afford to be off line for
very long. Santovac is so expensive down here that I don't want to
contaminate the new oil.

thanks

Ritchie

Ritchie Sims phone: 61 9 3737599 ext 7713
Department of Geology fax: 61 9 3737435
University of Auckland
Private Bag 92019
Auckland
New Zealand




From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Wed, 18 Oct 1995 17:42:33 GMT+1200
Subject: Silicone Oil in Diffusion Pumps of Carbon Coaters

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Thanks to all who replied to my original query of October 10, it
seems that the overwhelming consensus is that if one wants to avoid
Si contamination of specimens (as I do, not all minerals contain Si),
one should avoid silicones. Most people seem to use Santovac 5.
So on to the inevitable next question, which is:
How the heck can I clean out the system in preparation for
changing to Santovac?
One user suggested heating it (off the system) with dishwash
detergent solution, but I'm pessimistic about the chances of this
removing silicone oil and sludge. I've heard a rumour of a suitable
solvent, but no details.

Has anybody got a good way?

I have to have my battle plan ready before I take the coater out of
service, as I have only one coater, can't afford to be off line for
very long. Santovac is so expensive down here that I don't want to
contaminate the new oil.

thanks

Ritchie

Ritchie Sims phone: 61 9 3737599 ext 7713
Department of Geology fax: 61 9 3737435
University of Auckland
Private Bag 92019
Auckland
New Zealand




From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Wed, 18 Oct 1995 17:27:53 GMT+1200
Subject: Re: EPMA powder sample prep

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} Date sent: Tue, 17 Oct 1995 13:44:03 +0100
} From: Robert McDonald {robert-at-geology.gla.ac.uk}
} Subject: EPMA powder sample prep
} To: Microscopy-at-Sparc5.Microscopy.Com

}
} I have been asked to find out how to prepare powder
} samples for microprobe alaysis and I wondered if anyone
} out there in Microland did this on a routine basis.
}
} Any pointers would be most helpful.
}
} I have had previous samples made into pellets using a
} press which was used to prepare IR spectroscopy discs
} but the results are rather disappointingly bumpy.
}
} Thanks in advance,
}
} Robert McDonald & Cameca SX50
} Dept of Geology & Applied Geology
} Glasgow University
} Glasgow
} SCotland
}
} robert-at-geology.gla.ac.uk


My understanding is that simply pressing powders is very unlikely to
produce the smooth horizontal surfaces neccessary for decent results.
I have occasionally managed to embed dusts down to about 50 microns
in epoxy resin in a mould, followed by the usual geological grinding
and polishing, and I'm on the verge of ordering some Spurr's resin
(used in biological fields, A R Spurr, J. Ultrastruct. Res., 26,31-42
(1969)) which is supposed to be very low viscosity. I'll be
interested in the other replies.

cheers

Ritchie
Ritchie Sims phone: 61 9 3737599 ext 7713
Department of Geology fax: 61 9 3737435
University of Auckland
Private Bag 92019
Auckland
New Zealand




From: John Millar :      JJMILL-at-bunyip.ph.rmit.edu.au
Date: Wed, 18 Oct 1995 17:40:19 EST-10
Subject: sem/edx

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To all in Cyber-microland :
Does anyone have experience /data on lifetime and operational quality
of Kevex EDX detectors which do not use LN2 ?
cheers
jjm

Professor John J. Millar
Head, Department of Applied Physics
Electron Microscope Unit
RMIT, GPO Box 2476V, MELBOURNE AUSTRALIA 3001
+613 660 2602 fax 613 660 3837
email jjmill-at-rmit.edu.au




From: f.m.haug-at-basalmed.uio.no (Finn-Mogens Haug)
Date: Wed, 18 Oct 1995 14:51:19 +0200
Subject: CCD's for TEM

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Message-Id: {199510181250.NAA29438-at-pons.uio.no}
X-Sender: finnmog-at-pons.uio.no
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To the query by Laurie Marks on Princeton Instruments cameras
for TEM (I have lost the original message). No pieces of wisdom,
I am sorry, but a question.

What is Princeton's offer for TEM-use? One of their TE-series
cameras, or the Pentamax, with adapters for which TEM's? At
what prices?? Do they offer their standard software/is it
adapted for use with TEMs?

For the last 5 years we have recorded digital images with
a video-format Gatan 673 MkII "Wide angle camera" from our
Philips CM10. We are curious about high-resolution cameras.

Thanks for any information, regards

Finn-Mogens Haug

University of Oslo
Institute of basic medical sciences
Department of anatomy E-mail: f.m.haug-at-basalmed.uio.no
Box 1105 Blindern Phone : +47 22 85 12 67
N-0317 Oslo, NORWAY Fax : +47 22 85 12 78






From: kaurin-at-rmslab.rockefeller.edu
Date: Wed, 18 Oct 1995 10:23:47 EDT
Subject: Best Microtome

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What is the best microtome to purchase? I have used and enjoyed
an Ultracut E. However, they are no longer being made. Leica now has a
motorized/digital Ultracut S which I currently use. Although it is a nice
machine, it is difficult to face quickly with a glass knife due to the greater
resistance of the wheel. (my samples will fracture with razorblade facing)
Another facility has asked for our input on a microtome purchase. They are
considering a RMC MT-7000. Is this a good model? Are there any used ultracut
Es for sale? I look forward to hearing your views and suggestions.
Shelley Landon Kaurin






From: roy-at-bayou.uh.edu (Roy Christoffersen)
Date: Wed, 18 Oct 1995 08:58:13 -0500
Subject: Removing DC silicone HV grease: Final answer

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Thanks to all who offered suggestions for my problem regarding removing Dow
Corning silicone HV grease from our Gatan double-tilt holder. After talking
more than once with Dow Corning, as well as with technical support at
Gatan, I am please to report that solvents for this grease do exist. They
are either: methyl ethyl ketone, mineral spirits, methyl isobutyl ketone,
or a commercial solvent from Amtex Chemical in West Chester PA
(610-436-4813). Of these I tried methyl isobutyl ketone on grease smeared
on a glass slide and this worked in fairly short order. Jeff Gronsky at
Gatan reported good success using mineral spirits in an ultrsonic with
later cleaning in freon.
So that, as they say, is that.

Roy Christoffersen
Texas Center for Superconductivity
3201 Cullen
Houston, TX 77204-5932
roy-at-bayou.uh.edu
(713) 743-8273
FAX: (713) 743-2787






From: ldm2-at-apollo.numis.nwu.edu (L.D.Marks)
Date: Wed, 18 Oct 1995 10:43:15 -0500
Subject: Re: CCD's for TEM

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I think that pricing of CCD's as with everything, is a question
of negotiation. For that you will have to contact Princeton
directly (although I will do it for anyone for a commission!)




From: Xianying Burany :      xianying-at-UDel.Edu
Date: Wed, 18 Oct 1995 12:36:06 -0400 (EDT)
Subject: Subscribe

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I would like to subscribe. Thank you

Sandy Burany
xianying-at-strauss.udel.edu




From: Michael Rock :      merock-at-u.washington.edu
Date: Wed, 18 Oct 1995 09:21:30 -0700 (PDT)
Subject: Re: Best Microtome

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Shelley-
I share your opinion about the Ultracut E, it was the best ever built.
The simplicity, reliability and dependability of these machines was much
like the VW Bug, which was also discontinued (for a while) due it's long
lifetime. I would pursue the used Ultracut E.
Good luck!
-Mike

On Wed, 18 Oct 1995 kaurin-at-rmslab.rockefeller.edu wrote:

} What is the best microtome to purchase? I have used and enjoyed
} an Ultracut E. However, they are no longer being made. Leica now has a
} motorized/digital Ultracut S which I currently use. Although it is a nice
} machine, it is difficult to face quickly with a glass knife due to the greater
} resistance of the wheel. (my samples will fracture with razorblade facing)
} Another facility has asked for our input on a microtome purchase. They are
} considering a RMC MT-7000. Is this a good model? Are there any used ultracut
} Es for sale? I look forward to hearing your views and suggestions.
} Shelley Landon Kaurin
}
}
}




From: Sadowski :      sadowski-at-msmail.muohio.edu
Date: 18 Oct 1995 15:49:14 -0500
Subject:

Contents Retrieved from Microscopy Listserver Archives
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Message-Id: {199510181944.OAA07054-at-Sparc5.Microscopy.Com}

Subscribe Laura Sadowski {sadowski-at-msmail.muohio.edu}




From: GVKM07A-at-PRODIGY.COM (DR CHARLES A GARBER)
Date: Wed, 18 Oct 1995 10:04:38 EDT
Subject: EPMA Powder Sample

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-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --

There has been some recent discussion on the subject of mounting powder
samples for EPMA. I would like to present an alternative approach, one
in fact we believe to be superior, that being through the use of our
own "Tacky Dot Slide" product family.

No point in making a commercial here, you can obtain a full description
of SPI's Tacky Dot slides directly from the SPI Supplies WWW site given
below. From the "home page", just "click" on "new products" and then
"click" on "Tacky Dot" slides.

For EPMA preparation, what is needed is called the "Circle Analytical
Mount" holder which permits one to mount (in your favorite plastic) the
particles in a perfect orthogonal array, and then after polishing down
to cross sections, and if the EPMA system being used has stage
automation, then the analysis can be done completely automatically,
without human intervention.

The use of this system permits more accurate data that can be obtained
in a far shorter period of time.

You also have to select a "Tacky Dot" slide with a dot size that would
be appropriate for the particle size at hand. Generally speaking the
method is appropriate for particles larger than about 6-8 um and not
larger than about 1000um. There are currently eight different dot
sizes from which to select.

Chuck

======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail:
GVKM07A-at-prodigy.com
West Chester, PA 19381-0656 USA Customer Service:
SpiSupp-at-aol.com

########################################################
WWW: http://mail.cccbi.chester.pa.us/spi/spihome.html
########################################################
======================================================





From: bmenco-at-casbah.acns.nwu.edu (Bert Menco)
Date: Wed, 18 Oct 1995 16:22:31 -0500
Subject: Please remove

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Message-Id: {199510182122.AA151741347-at-casbah.acns.nwu.edu}

Please, remove my name from the list. I wasn't aware that I would get so
many messages, which are really of no use to me. I think the idea is great,
but we need still some time so that messages can better allocated to the
appropriate person. Thanks for courtesy of removing my address,

Sincerely,

Bert Menco, Ph. D.
(Research Associate Professor)





From: richard.easingwood-at-stonebow.otago.ac.nz (Richard Easingwood)
Date: Thu, 19 Oct 1995 09:41:45 +1100
Subject: Freeze-slamming cultured cells - methods?

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We would like to freeze-slam cultured cells grown on melanex,
cryosubstitute them, then infiltrate at low temperature before resin
embedding.

We have a metal mirror (MM80) freeze slam system which we would use.

Has anyone out there done this or does anyone know of some good references
on freeze slamming methods?

Thanks in advance
Richard

Please reply to: richard.easingwood-at-stonebow.otago.ac.nz

Richard Easingwood
South Campus Electron Microscope Unit
Otago Medical School
PO Box 913
Dunedin
NEW ZEALAND

Telephone: 64-03-479 7301
Facsimile: 64-03-479 7254

The southernmost electron microscope unit in the world






From: John Millar :      JJMILL-at-bunyip.ph.rmit.edu.au
Date: Thu, 19 Oct 1995 08:44:04 EST-10
Subject: Re: sem/edx w/o LN2

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} On Wed, 18 Oct 1995, I wrote:
}
} } To all in Cyber-microland :
} } Does anyone have experience /data on lifetime and operational quality
} } of Kevex EDX detectors which do not use LN2 ?

I am happy to collect and post summary of responses. Thanks to all
private replies requesting same.
Cheers
jjm
Professor John J. Millar
Head, Department of Applied Physics
Electron Microscope Unit
RMIT, GPO Box 2476V, MELBOURNE AUSTRALIA 3001
+613 660 2602 fax 613 660 3837
email jjmill-at-rmit.edu.au




From: Liang, Long :      LLIANG-at-is.arco.com
Date: 18 Oct 1995 13:18:13 CST
Subject: FW: EPMA powder sample prep

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Message-Id: {MACMS.LLIANG.405535130095291FMACMS-at-IS.ARCO.COM}



As far as I know, the procedure of analysis of powder samples is same as that of
analysis of microparticle samples. Dr. John Armstrong of CalTech has published
numerous papers about EPMA quantitative elemental analysis of microparticles.
He also designed a particle analysis program. One of his articles "Quantitative
elemental analysis of individual microparticles with electron beam instruments"
is in the book ELECTRON PROBE QUANTITATION, edited by Heinrich and Newbury
(1991).

Good luck!

Long Liang
ARCO EPMA/SEM Lab
Plano TX
_______________________________________________________________________________

} Date sent: Tue, 17 Oct 1995 13:44:03 +0100
} From: Robert McDonald {robert-at-geology.gla.ac.uk}
} Subject: EPMA powder sample prep
} To: Microscopy-at-Sparc5.Microscopy.Com

}
} I have been asked to find out how to prepare powder
} samples for microprobe alaysis and I wondered if anyone
} out there in Microland did this on a routine basis.
}
} Any pointers would be most helpful.
}
} I have had previous samples made into pellets using a
} press which was used to prepare IR spectroscopy discs
} but the results are rather disappointingly bumpy.
}
} Thanks in advance,
}
} Robert McDonald & Cameca SX50
} Dept of Geology & Applied Geology
} Glasgow University
} Glasgow
} SCotland
}
} robert-at-geology.gla.ac.uk









From: roger-at-teleport.com (Rufus Knapp)
Date: Wed, 18 Oct 1995 18:12:03 +0000
Subject: subscribe

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From: Jay Jerome :      jjerome-at-isnet.is.wfu.edu
Date: Wed, 18 Oct 1995 15:48:08 -0400 (EDT)
Subject: Re: Best Microtome

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With regard to microtomes, I learned along time ago that one persons
diamond is another's lump of carbon. I personally love the Ultracut E and
wish it was still available. However, I would suggest getting each of the
microtome companies to let you try their microtome for a couple of days
on your own material before purchasing. Everyone makes a good microtome,
it is a matter of individual taste which you prefer.


Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-isnet.is.wfu.edu *
**************************************************************

On Wed, 18 Oct 1995 kaurin-at-rmslab.rockefeller.edu wrote:

} What is the best microtome to purchase? I have used and enjoyed
} an Ultracut E. However, they are no longer being made. Leica now has a
} motorized/digital Ultracut S which I currently use. Although it is a nice
} machine, it is difficult to face quickly with a glass knife due to the greater
} resistance of the wheel. (my samples will fracture with razorblade facing)
} Another facility has asked for our input on a microtome purchase. They are
} considering a RMC MT-7000. Is this a good model? Are there any used ultracut
} Es for sale? I look forward to hearing your views and suggestions.
} Shelley Landon Kaurin
}
}
}




From: STEELE-at-KRDC.INT.ALCAN.CA
Date: Wed, 18 Oct 1995 17:46:08 -0500 (EST)
Subject: CCD for TEM, Priceton, etc.

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--Boundary (ID mdjLRiOs/Uc/lMX0vgYD2g)
Content-type: TEXT/PLAIN

Speaking of Princeton Instruments, I just had a demo of their "Micromax"
cooled CCD digital acquisition system a couple of days ago.

It took all of fifteen or twenty minutes to vent, bolt on, and pump down the
35mm port version on my Philips 400T, and start collecting images.
Considering most of the demos we've had here, I was darned impressed. Oh,
yeah, the image quality was excellent as well.

I tested the YAG scintillator type rather than the phosphor. The system
mates Princeton's camera system with a microscope interface furnished to
them by Fullam. I'll let them do the selling job on the "advanced" features,
but I will recommend giving them a look.

The cost on my quote is comparable with their only competitor's (that I know
of) in this price range (AMT), namely in the $60K Can. range.

I'm still trying to arrange a demo of AMT's offering to make my final
selection, but I'm convinced by what I've seen so far that this type
of system will certainly be suitable for our routine TEM imaging needs (i.e
materials BF, DF, SADPs).

Princeton Instruments Canada, BTW is at (613) 836 1074 (FAX)
or 76261.2210-at-compuserve.com

AMT is at (508)948-5507 (FAX)

If I've missed another candidate, I'd appreciate someone letting me know.

###############################################################
# #
# Don Steele STEELE-at-KRDC.INT.ALCAN.CA #
# ALCAN INTERNATIONAL #
# Kingston Research and Development Center #
# P. O. Box 8400 #
# Kingston, Ontario Canada K7L 5L9 #
# #
###############################################################

--Boundary (ID mdjLRiOs/Uc/lMX0vgYD2g)--




From: Kerry Gascoigne :      Kerry.Gascoigne-at-cc.flinders.edu.au
Date: Thu, 19 Oct 1995 14:02:12 +0930
Subject: Re: Silicone Oil in Diffusion Pumps of Carbon Coaters

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Message-Id: {9510190338.AA24661-at-gamgee.cc.flinders.edu.au}
Comments: Authenticated sender is {mnklg-at-[129.96.250.33]}

} Thanks to all who replied to my original query of October 10, it
} seems that the overwhelming consensus is that if one wants to avoid
} Si contamination of specimens (as I do, not all minerals contain Si),
} one should avoid silicones. Most people seem to use Santovac 5.
} So on to the inevitable next question, which is:
} How the heck can I clean out the system in preparation for
} changing to Santovac?
} One user suggested heating it (off the system) with dishwash
} detergent solution, but I'm pessimistic about the chances of this
} removing silicone oil and sludge. I've heard a rumour of a suitable
} solvent, but no details.
}
} Has anybody got a good way?
}
} I have to have my battle plan ready before I take the coater out of
} service, as I have only one coater, can't afford to be off line for
} very long. Santovac is so expensive down here that I don't want to
} contaminate the new oil.
}
The "official" method of cleaning diffusion pumps in anticipation of using
Santovac 5 was supplied to me by Edwards Australian Agents ten years ago.
The procedure is
" 1. empty the old oil charge.
2. block the outlet.
3. Fill the pump with a charge of 'Genklene'
4. Put glass plate on the top flange to seal.
5. Switch on the heaters and continue till the vapour reaches the top glass
plate.
6. Turn on the cooling water and turn off the heaters.
7. Wait till the pump cools before opening
8. repeat.
9.pour out the Genklene and recharge with santovac 5.

for any degreasing use carbon tet.
Due to the highly toxic fumes produced when carbon tetrachloride is heated,
Genklene is recommended as a safer cleaning fluid in the pump boiler."

Kerry Gascoigne
Flinders Microscopy and Image Analysis Facility




From: Kerry Gascoigne :      Kerry.Gascoigne-at-cc.flinders.edu.au
Date: Thu, 19 Oct 1995 16:36:53 +0930
Subject: Re: Silicone Oil in Diffusion Pumps of Carbon Coaters

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Message-Id: {9510190612.AA27002-at-gamgee.cc.flinders.edu.au}
Comments: Authenticated sender is {mnklg-at-[129.96.250.33]}



for any degreasing use carbon tet.
Due to the highly toxic fumes produced when carbon tetrachloride is heated,
Genklene is recommended as a safer cleaning fluid in the pump boiler."

Kerry Gascoigne
Flinders Microscopy and Image Analysis Facility





From: t.bostrom-at-qut.edu.au (Thor Bostrom)
Date: Thu, 19 Oct 1995 18:43:50 +1000
Subject: Re: Graphics file converters for PC

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In a previous message about PC {-} Mac image file conversion, I mentioned a
PC program called Image Alchemy, which is able to convert some 70! graphics
file formats. Sorry I didn't give a source site for it (I found the demo
version on a Shareware CD-ROM), so here it is.

Image Alchemy is produced by Handmade Software, Inc., who have a web site at
http://www.handmadesw.com/hsi/products.html
which also gives ordering info. A demo version can be downloaded from
ftp://ftp.handmadesw.com/pub/alchemy/
(the MSDOS version file is alch18.zip 677Kb)

To avoid confusion, I should mention that there's also a company called
Alchemy Mindworks Inc. who produce the well-known Graphic Workshop and other
graphics software. Their web page is at
http://www.north.net/alchemy/alchemy.html
and they give FTP sites for their shareware as
uunorth.north.net (directory /pub/alchemy)
and FTP mirror sites at ftp.rose.com and www.visi.com

I have no connection with these companies, just tried some of the software.

Thor Bostrom
Analytical EM Facility, QUT
Brisbane, Australia

(PS: Because of a problem with our university mail server I haven't been
receiving any Microscopy mail recently. I'm sending this out into the void.)







From: Tom Taylor :      ttaylor-at-msai.mea.com
Date: Thu, 19 Oct 1995 08:41:53 -0400
Subject: Unsubscribe

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Please unsubscribe ttaylor-at-msai.mea.com.
--
Regards,

Tom Taylor {ttaylor-at-msai.mea.com}




From: Sadowski :      sadowski-at-msmail.muohio.edu
Date: 19 Oct 1995 09:25:31 -0500
Subject: RE:subscribe

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Message-Id: {199510191338.IAA08227-at-Sparc5.Microscopy.Com}

OK, OK I get the message that I sent my subscribe message to the
wrong address. About a dozen people have sent messages! I would
have thought that the SysOp would be the only one to send a message.
Sending to the correct address now............. Please do not send
out any more wrong address messages! L.Sadowski




From: EMLAB-at-OPUS.MCO.EDU
Date: Thu, 19 Oct 1995 09:01:45 -0500 (EST)
Subject: Re: Best Microtome

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Dear Shelley,

I use an Ultra-cut S and have found it to be a very reliable machine.
I do not understand your comment about it taking to long to face a block
(what kind of block?) due to the resistance of the wheel. What does this mean?
If your samples fracture with razor blade facing, than why would microtome
facing be worse?, I would think it would be better because you con control
not only thickness of facing cuts but also speed.
I have no experience with the RMC so no comment on them.

Best of Luck,
Ed Calomeni
Medical College of Ohio
Toledo, OH
emlab-at-opus.mco.edu




From: Ilene Sugino :      suginoik-at-UMDNJ.EDU
Date: Thu, 19 Oct 1995 09:21:00 -0400 (EDT)
Subject: film recorders

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Message-Id: {199510191322.IAA08215-at-Sparc5.Microscopy.Com}

We are interested in purchasing a film recorder to output confocal images
and SEM images on to film. I would like to get feedback from the group
about their experiences with high resolution recorders. In particular, it
seems that some of the film recorders do well with graphics (text,
graphs, etc.) but distorts images.

I have posted this question to the confocal listserver and only got one reply.
So far, it looks like the Polaroid and Mirus recorders are not acceptable.

Thanks in advance for any comments and suggestions.--

Ilene

------------------------------------------------------------------------------

Ilene Sugino e-mail: suginoik-at-umdnj.edu
UMDNJ-Ophthalmology phone: (201) 982-7746
DOC 6th Floor fax: (201) 982-7762
90 Bergen Street
Newark, New Jersey 07103

------------------------------------------------------------------------------




From: ENERGY BEAM SCIENCES, INC :      75767.640-at-CompuServe.COM
Date: 19 Oct 95 12:23:39 EDT
Subject: Re: 35mm camera adapters

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Many years ago, we were asked by JEOL to design adapters to convert their SEMs
from 4 x 5 Polaroids to 35mm. We had a number of these made over the years, for
models ranging from the JEOL 35 to the 840 series. Before I convert them into
very snazzy black lucite birdhouses, is there anyone out there who would like to
buy one (cheap!)?
Steven Slap, Vice-President
Energy Beam Sciences, Inc.





From: ENERGY BEAM SCIENCES, INC :      75767.640-at-CompuServe.COM
Date: 19 Oct 95 12:23:39 EDT
Subject: Re: 35mm camera adapters

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Many years ago, we were asked by JEOL to design adapters to convert their SEMs
from 4 x 5 Polaroids to 35mm. We had a number of these made over the years, for
models ranging from the JEOL 35 to the 840 series. Before I convert them into
very snazzy black lucite birdhouses, is there anyone out there who would like to
buy one (cheap!)?
Steven Slap, Vice-President
Energy Beam Sciences, Inc.





From: wise-at-vaxa.cis.uwosh.edu
Date: Thu, 19 Oct 1995 13:12:55 +0000
Subject: job ad

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This just came to my attention:

Madison Area Technical College (Madison, Wisconsin) has openings for one or
more part-time EM instructors. Contact MATC Human Resources Office, PO Box
7128, Madison, WI 53707-7128 (608/246-6900) for application forms and
Affirmative Action forms.

DUE DATE FOR RECEIPT OF APPLICATION MATERIALS IS NOV 3, 1995.

Good luck.

Bob






From: jfmjfm-at-umich.edu (John F. Mansfield)
Date: Thu, 19 Oct 1995 10:33:10 -0400
Subject: Graphics Converters

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The one I like is
"Grpahic Converter" It is by Thorsten Lemke.
Lemke Software
Insterberger Str. 6
31228 Peine
Germany
100102.1304-at-compuserve.com

This converts almost every file format I know to everyother format. Has
some glitches, but is a really nice program.

The version I have is 2.15. It is shareware and you can FAX the order form
to him and give a credit card number. I have registered (havent heard from
him yet tho').

John Mansfield
North Campus Electron Microbeam Analysis Laboratory
413 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (313)936-3352 FAX (313)936-3352
jfmjfm-at-engin.umich.edu, John.F.Mansfield-at-umich.edu
or jfmjfm-at-umich.edu they all reach me!
URL: http://wwwpersonal.engin.umich.edu/~jfmjfm/jfmjfm.html






From: Richard E. Edelmann :      EDELMARE-at-CASMAIL.MUOHIO.EDU
Date: Thu, 19 Oct 1995 16:15:37 -500
Subject: Re: film recorders

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I too am interested in doing very similar things. The name i have
ceom up with is Laser Graphics LFR (Lasergraphics Film Recorder) at
714-753-8282. Their options are:

LRF - Personal Plus
- Permanantly mounted 35 mm camera back
- 33-bit color depth output (sorry I don't know the max resolution)
- $6,000 US

LFR X-95
- Three switchable cammera backs: 35mm Standard, 35mm Bulk film,
and polaroid 4x5.
- 33-bit color depth
- $7,000 US

LFR Mark II
- Standard 35mm, 35mm bulk film, polaroid, 120, 220, or 4x5
- 36-bit color depth
- $10,000 US

LFR MARK III
- Standard 35mm, 35mm bulk film, polaroid, 120, 220, or 4x5
- 36-bit color depth upto 8k x 8k resolution
- $20,000 US


I know that these systems are designed for DOS/Windows PC systems, I
do not know if other systems are also possible.

Hope that helps some what.





From: Gib Ahlstrand :      giba-at-puccini.crl.umn.edu
Date: Thu, 19 Oct 1995 14:20:08 -0500
Subject: Re: cleaning diff pump

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Richie Sims wrote:

} ......... Most people seem to use Santovac 5.
} So on to the inevitable next question, which is:
} How the heck can I clean out the system in preparation for
} changing to Santovac?
} One user suggested heating it (off the system) with dishwash
} detergent solution, but I'm pessimistic about the chances of this
} removing silicone oil and sludge. I've heard a rumour of a suitable
} solvent, but no details.

Richie: When I clean out my vacuum evaporator (about every 3-4 years) after
washing off "wet" oil coating from the diffusion pump casing and stage
stack(disassembled) with hot detergent solution as described above followed by
acetone rinse, I then take the dried parts - diff pump casing, stack parts -
over to my campus Scientific Apparatus shop where they use a glass bead blaster
to remove the burned on dark deposits that are virtually impossible to remove
with detergents or by scrubbing. It works beautifully, the bead stream quickly
"melts" the deposits off leaving a fresh clean metal surface that your new oil
charge will just love to boil up against!

Try to find a shop with a bead blaster. You may have to go to your local auto
body shop if your facility has none available. Hope this helps. Gib



--

Gib Ahlstrand, MMS Newsletter Editor
Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
495 Borlaug Hall, St. Paul, MN 55108 (612)625-8249
612-625-9728 FAX, giba-at-puccini.crl.umn.edu

"MICROSCOPY & MICROANALYSIS 96" in Minneapolis, Minnesota, Aug.11-15, 1996





From: dshubito-at-d.imap.itd.umich.edu (Dennis Shubitowski)
Date: Thu, 19 Oct 1995 17:41:46 -0400
Subject: Re: Best Microtome

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I came from a imaging core that had two RMC MT7s and a Reichert
Ultracut S to a facility with only an RMC MT6000XL. At my former
workplace, the difference was night and day -- the Reichert was a
beautiful instrument with no troubles experinced at all (except the
under-lit bulb tends to burn out often unless you replace it with
the longer life filaments.) The RMC, however, was, to put it
gently, a piece of garbage. Constant problems with the RMC service
taking much too long, and the microtome simply did not seem well built.
At one point, the microtome was out of service for about month while
waiting for the engineer to show up. When he finally did, it took
another week or two to fix the problem. I can elaborate further on
the problems we had, but I think we just bought a lemon.

On the other hand, I have since been cutting on the MT6000XL, and
it has been *very* nice. I have no complaints about the microtome,
although hopefully it will not be in need of service anytime soon.

Other comments are that the RMC feels more like a "traditional"
microtome with the large wheel, whereas the Reichert has a very
small wheel that takes some getting used to. Also, all the controls
for the RMC are within the console while the Reichert has them on
a separate unit. The overhead flourescent light on the Reichert is
fixed in place, which can be both good and bad. Good because the
light on your thins is always consistent; bad because sometimes you
want to move it depending on the placement of your diamond knife.

Regardless, before you make your decision, I would definitely see if
you can use each product on a "loaner" basis just to get a feel for
each.

Good luck with your decision!

Dennis Shubitowski
dshubito-at-umich.edu






From: David Leaffer (415)852-1828 :      David.Leaffer-at-SYNTEX.COM
Date: Thu, 19 Oct 1995 12:26:12 -0800 (PST)
Subject: RE: film recorders

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MR-Received: by mta RVAX.MUAS; Relayed; Thu, 19 Oct 1995 12:26:12 -0800
MR-Received: by mta MEDEC; Relayed; Thu, 19 Oct 1995 12:26:13 -0800
MR-Received: by mta CHUB1; Relayed; Thu, 19 Oct 1995 12:25:49 -0800
Disclose-recipients: prohibited

I have use two film recorders the Polaroid Digital palette and the Focus
Graphics analog Imagecorder.

The Polaroid has been a disapoinment. Small lines and text tend to dissapear
in the slide. Also, we have had problems with washing out of portions of the
slide because some color combinations don't work well together. This seems to
be a problem of the small CRT used in the system.

The Polaroid system also requires a third party driver. Most of the problems
with the driver seem to be resolved now, but there have been headaches with
this also. The problem arises with objects that the driver can't convert. It
also takes from 3-5 minutes for printing each slide.

Recently analog (splits the signal going to the monitor) slide makers that work
at the PC screen frequency have become available. Previously they only worker
with high end work stations .

The system we have has worked very well on our Pentium system. The resolution
is excellent, anything displayed on the monitor shows up on the slide exactly
the same, and it takes only a few seconds to print each slide. There is a
slight jagged look to the text compared to the Post Script Polaroid system. (We
have printed graphics, scanned images, gels, etc.)

David Leaffer
Roche Bioscience
david.leaffer-at-syntex.com







From: STEELE-at-KRDC.INT.ALCAN.CA
Date: Thu, 19 Oct 1995 14:44:53 -0500 (EST)
Subject: Re: CCD for TEM-SADP/YAG lifetime

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--Boundary (ID NnMHz7F3QURC38CHIVJ2bw)
Content-type: TEXT/PLAIN

In response to Philippe Buffat's comments, I WAS concerned about damage to
the scintillator in collecting SADPs. The patterns I collected were with the
beam stop in place. In the salesman's place, I likely would have requested
that that particular acquisition type not be tested. Admitedly, I was taking
advantage of what was likely inexperience with TEM on the part of the
salesperson.

The benefit of collecting some patterns this way are that you can use the 12
bits of range in the image (in the case of the camera I was testing)to see
faint spots which can be difficult to image and record (superlattice spots
and the like).

Our facility is a multiuser lab, but with limited, trained users. I figure
that the speed, dynamic range, ease of data sharing benefits will outweigh
the risk of damage.

The manufacturer is certainly not going to warranty scintillator life, but
then it won't take very many fried scintillators for me to outlaw SADP
collection on a system. The plate film camera will always be available for
that anyway.

} From: IN%"philippe.buffat-at-cime.uhd.epfl.ch" 19-OCT-1995 13:44
} To: IN%"STEELE-at-KRDC.INT.ALCAN.CA"
} Hello,
}
} Don Steele said that he had good test for recording BF,DF and SADPs with a
} cooled CCD camera (YAG screen).
}
} I am glad to hear this. We are many to think that we should stop with
} photographic films for many applications. But I am also really surprised.
}
} To my knowledge, until now, no slow scan CCD supplier has accepted to
} warranty the YAG life when it is used for SADPs.
}
} They all say that you can record SADPs only if you properly hide the (000)
} transmitted beam with a beam stop or if this beam IS NOT TOO BRIGHT (!??).
} They also say that when this beam hits the YAG target, it will
} irreversibly damage (decompose) it within about a few tenths of a second if
} the sample is thin and the pattern well focused.
} At least in a multi-user environment, nobody can exclude that somebody
} forgets to put the beam stop or that the (000) moves out of it
} (charging/magnetic effect). Who is really ready to take the risk?
}
} Is Micromax a more resistant camera/screen than competitors, or is
} Princeton Instruments not aware of the risk, or are the others competitors
} too cautious?
}
} Moreover, if you record a SADP with bright difracted spots, you may see a
} memory (remanence) effect on the next DF images. Gatan told me last week
} that you can get read of this memory effect by flooding the YAG with a
} uniform BRIGHT illumination for about 10 s after each diffraction pattern.
} No doubt it works fine for the camera, but would you consider that these
} operating conditions are the best for DF (LOW light level) observation (and
} the operator's eyes health.
} __________________________________________________________________
} Philippe Buffat Ecole Polytechnique Federale de
} Lausanne (EPFL)
} Centre Interdepartemental
} de Microscopie Electronique
} Address: EPFL-CIME, Batiment MX-C, CH-1015 Lausanne, Switzerland
} Phone: +41(21)693 29 83 Fax: +41(21)693 44 01 (Central European Time)
} E-mail: philippe.buffat-at-cime.uhd.epfl.ch // pabuffat-at-cimesg1.epfl.ch
} ______________________________ Eudora F2.1 ___________________________
}


--Boundary (ID NnMHz7F3QURC38CHIVJ2bw)--




From: John G Humenansky :      humen001-at-maroon.tc.umn.edu
Date: Thu, 19 Oct 1995 20:13:17 -0500 (CDT)
Subject: Re: Silicone Oil in Diffusion Pumps of Carbon Coaters

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A vapor degreaser will do a nice job of cleaning a diffusion pump
provided that the DP will fit into the degreaser. First disassemble the
DP, remove the stack and clean with acetone prior to using the vapor
degreaser. Dissassemble the stack unless it is a one piece type and also
clean it with acetone. The stack is usually the hardest to clean and you
might consider bead blasting followed by a thourough polishing to remove
the Si bead residue that might become embedded into the softer stack
metal from the bead blaster.

The roughing and backing lines can be cleaned by pulling saturated cloths
through the lines with multi conductor electrical wire of 14-18 ga. This
may need to be repeated several times starting with petroleum ether and a
final cleaning of acetone.

The roughing and backing valves should be disassembled and thouroughly
cleaned with petroleum ether and acetone. This cleaning and overhaul is
also a good time to replace all of the "O" rings with viton seals.

The vacuum chamber should be cleaned and if the system has a LN2 baffle
or trap this should also be cleaned with petroleum ether and acetone.

Excercise patience, as this can be a messy task but it will be worth your
effort. Have adequate ventilation and avoid breathing vapors by using
the acetone and petroleum as sparingly as possible.

Hope this helps.

John Humenansky
Advanced Research Corp.
612-331-2211




From: Laszlo Komuves :      komuves-at-itsa.ucsf.edu
Date: Thu, 19 Oct 1995 21:54:24 -0700 (PDT)
Subject: TEM for sale

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We have an old ZEISS 10A TEM (serial # 4684) for sale. This scope is
still used, in excellent working condition (considering its age), and has
always been on service contract. Nothing special, not even a goniometer
holder, but it is well stocked with spare parts.
We need the space for a new microscope, therefore in about a month this
scope will be de-installed and put into storage.
Asking price is $5000 or best offer, this includes the pumps and the
water chiller.
For further information contact me.
Sincerely,
Laszlo G. Komuves, PhD
Director, Morphology Laboratory
Department of Dermatology
University of California San Francisco, and VA Medical Center
Phone: (415)221-4810, Ext.3720
Fax: (415)751-3927




From: MELSEN :      MELSEN-at-MICROBIO.emory.edu
Date: Fri, 20 Oct 1995 7:49:27 EST
Subject: RE: film recorders

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Having had a Presentation Technologies FR1 for more than five years, I
have found that the output from video sources to this unit is adequate for
projection slides but not acceptable for negatives. On an opposite note
positives and negatives generated from a computer are quite acceptable. Tis
unit is extremely slow but very good resolution. The new FR2 model is
faster but not as sharp for some unknown reason.

Reviewing the currrent market for a unit which will output at peak
resolution (2K, 4K, 8K) for both positives and negatives in multiple formats
( 35mm to 120 to bulk) the OPAL from MGI of Minneapolis ( 612 854
1222) is far and away the winner. This unit comes with a hefty price ,
around $16K with software. If price is no object, this is the best in the
medium range. Others in the commercial range, above $25K, do offer
faster print times, higher line scan rates, larger bulk loaders.

The recorders from AGFA are all acceptable, albeit very slow, and not as
user friendly, in the under $10K price range. The medium price range
($15K+) is even less user friendly to the occasional operator.

The Lasergraphics units do have a nice positive output, but fall down on the
negative output. This is the most often purchased unit in this area for
making slides for presentation as of this date.

Polaroid is amateur material for the non-microscopist market.

Regards ,
melsen-at-MICROBIO.emory.edu
L.R. Melsen
Emory University
Microbiology and Immunology
3029 Rollin Research Center
Atlanta, Ga 30322
404 727 3508 FAX 404 727 3659




From: Fermin, Cesar :      fermin-at-TMC.Tulane.Edu
Date: 20 Oct 1995 07:15:34 -0500
Subject: Results DOS-MAC Syquest

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Message-Id: {n1397948390.78358-at-msmail.tmc.tulane.edu}

Results DOS-MAC Syquest:
Thanks to all those who responded to my inquiry. As you remember the problem
was not translating files but rather seeing (mounting) a DOS formatted Syquest
on a Mac desk top.
The good news is that I did not have to buy anything else. The Dos Mounter
multiMounter software can do it with no problem. However, the newer version
of PC access on the upgrade of Mac system 7.5 does it as well. The older
version (first release does not). Those of you using McLink Plus, should know
that if you upgraded from a system Mac system 6 to a 7 version, the old driver
(sticky) will put its signature on MOST DOS mounted media adding a MacLink
start up document...When this happens you can not just double chick on those
document to start the application that created them, because you may be up for
a surprise on what comes up on the screen.
On translation, yes I use for while Graphic converter. It is a Shareware
(pay your dues) and can be downloaded from
http://hyperarchive.lcs.mit.edu/HyperArchive/Archive/gst/grf/graphic-converter-215.hqx,
but make sure you have an installed version of Stuff or the like to convert
the binhex file into something you can use.
Also someone suggested getting the program Alchemy from
http://www.handmadesw.com/hsi/products.html, but I have not downloaded yet.
If you have any comments or questions please address them to my directly
rather than to the list.
******************************************************************
*Cesar D. Fermin, Ph.D \|T|/ Fax (504) 587-7389 *
*Tulane Medical School /|M|\ Voice Mail (504) 584-2618 *
*Pathology/SL79 \|C|/ Secretary (504) 584-2436 *
*New Orleans La 70112-2699 /|*|\ Lab/Techn. (504) 584-2521 *
* {Fermin-at-tmc.tulane.edu} Dir. Morphological Services *
******************************************************************




From: David Leaffer (415)852-1828 on Thu, Oct 19, 1995 8:45 PM
Date: 20 Oct 1995 07:03:30 -0500
Subject: RE: film recorders

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Message-Id: {n1397949110.34838-at-msmail.tmc.tulane.edu}

Here at Tulane/Pathology we used a MONTAGE (From Present. Techn. in Ca
408-730-3700) film recorder (DOS-MAC) for years with almost no problem. The
resolution is 4000lines (same as commercial labs. It takes time to learn a
few hard to learn tricks (at least in the older model), but the techn
assistance has been good. We literally recorded hundred of rolls with no
problems. Two problems deserve mention: a) If you use Power Point to makes
slides and fuse different styles, you could end up with a color background you
did not choose, b) The default size does not film the frame of a 35 mm film.
Thus, it has to be manually changed! I am not associated with the company
that makes the product and gives this information as a user's advice.
******************************************************************
*Cesar D. Fermin, Ph.D \|T|/ Fax (504) 587-7389 *
*Tulane Medical School /|M|\ Voice Mail (504) 584-2618 *
*Pathology/SL79 \|C|/ Secretary (504) 584-2436 *
*New Orleans La 70112-2699 /|*|\ Lab/Techn. (504) 584-2521 *
* {Fermin-at-tmc.tulane.edu} Dir. Morphological Services *
******************************************************************
_______________________________________________________________________________

I have use two film recorders the Polaroid Digital palette and the Focus
Graphics analog Imagecorder.

The Polaroid has been a disapoinment. Small lines and text tend to dissapear
in the slide. Also, we have had problems with washing out of portions of the
slide because some color combinations don't work well together. This seems to
be a problem of the small CRT used in the system.

The Polaroid system also requires a third party driver. Most of the problems
with the driver seem to be resolved now, but there have been headaches with
this also. The problem arises with objects that the driver can't convert. It
also takes from 3-5 minutes for printing each slide.

Recently analog (splits the signal going to the monitor) slide makers that
work
at the PC screen frequency have become available. Previously they only worker
with high end work stations .

The system we have has worked very well on our Pentium system. The resolution
is excellent, anything displayed on the monitor shows up on the slide exactly
the same, and it takes only a few seconds to print each slide. There is a
slight jagged look to the text compared to the Post Script Polaroid system.
(We
have printed graphics, scanned images, gels, etc.)

David Leaffer
Roche Bioscience
david.leaffer-at-syntex.com








From: GRAZUL-at-zodiac.rutgers.edu
Date: Fri, 20 Oct 1995 11:52:44 -0400 (EDT)
Subject: Baltec Vaporized!

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All,

I was absolutely blindsided yesterday with the news that Baltec is gone.
It seems that some old Balzers employees bought Baltec out and now
call themselves Techno Trade {I hope that its not Tech No Trade}. All
should be the same except for the great service that we got both in person
and over the phone; Al and Pat are gone and may not be back....I put in
a very good word for them to the new owners. Since I have six major
pieces of equipment from Balzers and no service contracts the help
I have recieved over the years, as well as the good humor, has saved
me thousands of dollars.

Please call the new owners at (603) 622-5011, to find out how the new
company impacts your lab and put in a good word for the old service
reps.

John Grazul
Rutgers University
(908) 445-5308




From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 20 Oct 1995 14:33:22 -0400
Subject: RE-SEM & Cleanliness

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Message-ID: {n1397922603.79026-at-mse.engin.umich.edu}

Subject: Time: 2:22 PM
OFFICE MEMO RE:SEM & Cleanliness Date: 10/20/95

I think it would make it possible for us to make a more relevant reaponse if
you would tell us what kinds of materials you are interested in detecting in
establishing whether or not your parts are "clean".





From: cook-at-aaem.amc.anl.gov (Russell E. Cook)
Date: Fri, 20 Oct 1995 16:15:45 -0500
Subject: Re: film recorders

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We have been using a Lasergraphics LFR-Plus film recorderfor 5 or more
years. It has the following characteristics:
A. Input file formats: PICT1, PICT2, TIFF, PhotoShop 2.0 and 2.5
B. Resolution and bit depth: 2048x1365 or 4096x2731 lines; 33 bit color.
C. ISO-100 film output: 35 mm or 4x5 inch (Polaroid and sheet flim).

It has been very reliable, and it produces good images, graphics, and text.
Lasergraphics also provides software updates via its BBS.


Russell E. Cook
Electron Microscopy Center for Materials Research
Argonne National Laboratory
9700 South Cass Avenue
MSD-212
Argonne, IL 60439
(708)252-7194
FAX: (708)252-4798






From: jbpawley-at-facstaff.wisc.edu (James Pawley)
Date: Fri, 20 Oct 1995 19:31:02 -0600
Subject: Preliminary 3D Microscopy Course Announcement.

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Message-Id: {v02130501acadfd9a576f-at-[144.92.55.134]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Preliminary announcement:

An intensive, 8-day course on

"3D MICROSCOPY OF LIVING CELLS"

will be given at the

University of British Columbia, Vancouver, BC, Canada

July 27 - August 4, 1996

Covering everything from basic microscopy to the technical considerations
that define the highest levels of performance of the confocal microscope,
this course will include:

* Quantitative confocal microscopy
* Pixelation: The Nyquist Criterion
* Lasers and laser tweezers
* Objectives and aberrations
* Scanning-systems
* Wide field/deconvolution techniques
* Detectors: operation and performance
* Optimal pinhole size & photon efficiency
* Dye design, characteristics and use
* How to keep your cells alive
* Two-photon excitation
* Video-rate confocal imaging
* Measuring ion concentrations
* Display and measurement of 3D data
* Digital hard copy and storage
* Fluorescent & gold labeling of living cells
* Backscattered light imaging.

Lecture demonstrations will be interspersed with hands-on laboratory
exercises that will utilize all of the currently available commercial
instruments for 3D microscopic imaging.
Students will work in groups of three throughout the discussion and
laboratory sessions, and will complete a live-cell 3D study on a specimen
of their choice.
At least seven, separate 3D microscopical workstations, attended by a
technical staff of 15, will be available for student use. Overall, the
teacher/student ratio will be more than 2:1.

International Academic Faculty:

* Jon Art University of Illinois
* Milton Charlton University of Toronto
* Rachel Errington Oxford University
* John Heuser Washington University
* Jim Pawley University of Wisconcon-Madison
* Wallace Marshall U. of California, San Francisco
* Ernst Stelzer EMBL, Heidelberg
* Roger Tsien University of California, San Diego
* Watt Webb Cornell University
* Michael Weis University of British Columbia
* Nick White Oxford University

International Commercial Faculty:

* Ernst Keller Carl Zeiss, NY
* Paul Millard Molecular Probes, OR
* Sigrid Myrdal Bristol-Myers Squibb, WA
* K. Sam Wells Bio-Rad,,CA

TUITION

Tuition is $1,500 US. On receipt of the deposit, all students will
receive preliminary assignments and a copy of the textbook, "Handbook of
Biological Confocal Microscopy," (Plenum, 1995).
The tuition fee includes one ticket for the opening reception and the
banquet, the textbook and all handouts. Accommodations and meals are not
included in the tuition fee.

APPLICATIONS

Applicants will complete a questionnaire to assess knowledge level and
field of interest. Enrolment will be limited to 20 participants.
Selection will be made on the basis of background and perceived need.
Those without previous LM experience will be provided with basic texts to
read before the course begins. Application packages may be obtained from


Prof. James Pawley, Rm. 1235,
1500 Johnson Dr., Madison, WI, USA 53706.
Phone: 1-608-263-3147, Fax 1-608-265-5315,
Email: jbpawley-at-facstaff.wisc.edu

Application deadlines:

Applications forms requesting information on field of interest and level
of experience must be received for screening by March 1, 1996.
Successful applicants will be notified by April 1, and a deposit of 50%
must be received by April 15, 1996. In general, refunds of the deposit
will not be possible.

DATES:

Applications must be received by Mar. 1/96
50% deposit due Apr. 15/96
Registration 3:00 - 5:00 pm Sat., July 27/96
Last class will end with lunch Sun., Aug. 4/96



*****************************************
Prof. James B. Pawley,
Ph. 608-263-3147
Room 1235, Engineering Research Building, FAX 608-265-5315
1500 Johnson Dr., Madison, WI, 53706 JBPAWLEY-at-FACSTAFF.WISC.EDU






From: Stephen A. Shaffer :      sshaffer-at-microdataware.com
Date: Thursday, October 26, 1995
Subject: SF Mic. Soc. Meeting Announcement

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3777 Depot Road, Suite 408
Hayward CA 94542

Abstract: The analysis of small mineral specimens presents
problems to the mineralogist. In the case of well-crystallized
micromounts, the specimen is small and only a limited amount of
material is available for analysis. Without the use of an
electron microprobe, the analyst is obliged to use chemical
microscopy techniques. These simple and often elegant tests
are perfect for the analyst on a budget. Normally a
combination of optical properties determination and a few
chemical tests are all that are needed for confirmation of a
mineral. Both these techniques require very small amounts of
material.
The techniques of "opening up," or soluabilization of
minerals, will be discussed. Some of the older blowpipe
methods are incorporated in the analytical scheme used in this
study. A step-by-step analytical approach to specific cases
will be outlined.

Directions to Forensic Analytical Specialties, Inc. (FASI):
FASI is located near the east end of the Hayward-San Mateo
Bridge, in a light industrial park at the corner of Depot and
Cabot Roads. From Highway 92, take the Clawiter Road exit,
travel north on Clawiter for approximately 3/4-mile to Depot
Road. Turn left (west) on Depot and go all the way to the end
of the road. Enter the first driveway past Cabot on your right
and drive straight into the parking lot. Forensic Analytical
is clearly marked on the building face. Look for signs
directing you to the appropriate door. FASI has expanded an
remodeled since we were last in their classroom, so the
location will be a new one for us.

Contact: For further information, contact Stephen A. Shaffer,
MicroDataware, 1-510-582-6624 (voice), 1-510-582-8295 (fax), or
by email to sshaffer-at-microdataware.com. Mark your calendar
NOW!!!!


************************************************************
* Stephen A. Shaffer * Publishers of The Particle Atlas *
* MicroDataware * on CD-ROM. Developers of custom *
* 2894 Tribune Avenue * image and database software for *
* Hayward CA 94542-1637 * laboratories, specializing in *
* 1-510-582-6624 voice * light and electron microscopy. *
* 1-510-582-8295 fax * Email inquiries invited. *
* sshaffer-at-microdataware.com *
************************************************************





From: smithj-at-acad.winthrop.edu
Date: Sat, 21 Oct 1995 07:07:59 -0400
Subject: MT-1 Specimen holders wanted

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Microscopists:
In an attempt to minimize wear and tear on our MT2b, I'm setting up an
old MT-1 for serial thick sectioning. The problem is that the specimen
holders are missing. Does any one out there have some tucked away?
E-mail me personally, rather than reply to the list.
TIA
Julian Smith III
Biology
Winthrop University
Rock Hill, SC 29733
smithj-at-winthrop.edu
803-323-2111 (vox)
803-323-2246 (fax)




From: Nestor J. Zaluzec :      zaluzec-at-Sparc5.Microscopy.Com
Date: Sat, 21 Oct 1995 16:52:05 -0500
Subject: Re: image simulation software on MSA Site

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There are copies of the original code for image simulation
of dislocations by the CSIRO group ( One Dis, & Two Dis)
in the MMSLib, which is available via the MSA ftp site.

Log in to the host site:

ftp.msa.microscopy.com

username = anonymous
password = your Email address

go to the directory called /1-Public/4-MMSLib/CTEM

look for the folders called ONEIDSAN and TWODISAN

You will have to get a local computer guru to help you
compile the code for your local computer platform.

It is all in Fortran, which only dinosaurs like me use...


Your Friendly Neighborhood SysOp

Nestor




From: f.lawrence-at-qut.edu.au (Felicity Lawrence)
Date: Mon, 23 Oct 1995 10:14:27 +1000
Subject: SEM to TEM

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Hi everybody.

We have a client who is looking at deformed endothelial cells on cornea.
He wants to look at them under SEM initially to locate their position and to
take photos. He then wants to examine them in the TEM to reveal more
information about their nature. Our problem is in marking the desired area
in some way so as to locate the position for TEM preparation.

We have tried burning around the deformed cells with the electron beam but
the results are not visible under LM so locating the cell(s) for TEM is
extremely difficult. We have tried placing a TEM labelled grid over the
area, looking for damaged cells in the grid holes, identifying their
position, then cutting the cornea at the desired level - technical
difficulties associated with this.

DOES ANYONE HAVE AN IDEA OF HOW WE MIGHT ACHIEVE OUR AIMS? IS THERE A TRIED
AND TRUE METHOD FOR THIS APPLICATION?

Preparation : Basically the lens is glut then OsO4 fixed (helps with
secondary electron emission), dehydrated in ethanol and CPD. Lenses are
mounted onto stubs and gold coated. The coating must be thick enough to
prevent charging and heat damage by the beam.

Many thanks,
Felicity

QUT, Australia





From: adm.business.manager-at-jcsmr.anu.edu.au (Vicki Sawyer)
Date: Mon, 23 Oct 1995 15:25:52 +1000 (EST)
Subject: SEM to TEM

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unsubscribe Microscopy-request





From: Ronald LHerault :      lherault-at-acs.bu.edu
Date: Mon, 23 Oct 1995 08:23:20 -0400 (EDT)
Subject: Tea

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Microscopy {Microscopy-at-aaem.amc.anl.gov} ,
Dixieland Group {dixielandjazz-at-islandnet.com}
Message-ID: {Pine.3.89.9510230832.E69753-0100000-at-acs3.bu.edu}
MIME-Version: 1.0
Content-Type: TEXT/PLAIN; charset=US-ASCII

I just used the last of my supply of King Cole Tea, a product of Canada.
Any members of theis list in Eastern Canada wh would be willing to send
me a box or two of the 250 sachet, gauze bag variety? I'd be happy to pay
expenses+.

Ron
Residence in Attleboro MA
lherault-at-acs.bu.edu





From: William Tivol :      tivol-at-wadsworth.org
Date: Mon, 23 Oct 1995 13:21:51 -0400 (EDT)
Subject: Re: cleaning diff pump

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X-Nupop-Charset: English

} I then take the dried parts - diff pump casing, stack parts -
} over to my campus Scientific Apparatus shop where they use a glass bead blaster
} to remove the burned on dark deposits that are virtually impossible to remove
} with detergents or by scrubbing. It works beautifully, the bead stream quickly
} "melts" the deposits off leaving a fresh clean metal surface that your new oil
} charge will just love to boil up against!
}
} Try to find a shop with a bead blaster. You may have to go to your local auto
} body shop if your facility has none available. Hope this helps. Gib
}
Dear Gib & all,
If you can't find a shop with a bead blaster, buy an air eraser kit
from Scientific Instrument Services (908) 788-5550. The kit was { $100 when
I bought one. You'll need a hood and compressed air. A small regulator for
the compressed air is also needed, unless that is built in to your air supply.
Good luck.
Yours,
Bill Tivol




From: masur-at-msvax.mssm.edu
Date: Mon, 23 Oct 1995 08:15:31 -0500
Subject: NYSEM meeting - November 1

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Message-Id: {199510231716.NAA20749-at-thomas.ge.com}
X-Authentication-Warning: thomas.ge.com: daemon owned process doing -bs

Wednesday, NOVEMBER 1, 1995

SPEAKER: Conly L. Rieder, PhD
Professor, SUNY Albany
Chief, Laboratory of Cell Regulation,
Wadsworth Center


TOPIC: Lasers, Microscopes and Mitosis:
Application of
Video-Enhanced Laser Microsurgery
to the Study of Chromosome Motion


5:30 PM
Mount Sinai School of Medicine
100th St between Fifth and Madison
Annenberg 25-51


Best regards,

Sandra K. Masur Box 1183
fax: 212-289-5945 Mount Sinai School of Medicine
phone: 212-241-6544 or 0089 NY NY 10029-6574
e-mail:masur-at-msvax.mssm.edu









From: Daniel Possin :      oemlab-at-u.washington.edu
Date: Mon, 23 Oct 1995 11:09:11 -0700 (PDT)
Subject: Re: Best Microtome

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X-Sender: oemlab-at-saul4.u.washington.edu

Hi -

I have been using an MT-6000 for several years now and am very satisfied
with it. I have only used a 7000 on demo, but thought it to be a very
nice machine and well worth the price. Good luck in your search.


On Wed, 18 Oct 1995
kaurin-at-rmslab.rockefeller.edu wrote:

} Date: Wed, 18 Oct 1995 10:23:47 EDT
} From: kaurin-at-rmslab.rockefeller.edu
} To: microscopy-at-Sparc5.Microscopy.Com
} Subject: Best Microtome
}
} What is the best microtome to purchase? I have used and enjoyed
} an Ultracut E. However, they are no longer being made. Leica now has a
} motorized/digital Ultracut S which I currently use. Although it is a nice
} machine, it is difficult to face quickly with a glass knife due to the greater
} resistance of the wheel. (my samples will fracture with razorblade facing)
} Another facility has asked for our input on a microtome purchase. They are
} considering a RMC MT-7000. Is this a good model? Are there any used ultracut
} Es for sale? I look forward to hearing your views and suggestions.
} Shelley Landon Kaurin
}
}
}

%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
% %
% Daniel Possin Work: 206/ 543-7489 %
% Dept. of Ophthalmology RJ-10 FAX: 206/ 543-4414 %
% University of Washington Home: 206/ 778-1714 %
% Seattle WA 98195 USA Email: oemlab-at-u.washington.edu %
% %
% "The chinese expression 'cheung meng ba sui, gong hey fat choy' is %
% equilvalent to Vulcan expression 'live long and prosper'. It's a %
% small universe and getting smaller everyday". %
% %
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%





From: wesaia-at-iastate.edu (Warren Straszheim)
Date: Mon, 23 Oct 1995 15:59:32 -0500
Subject: Re: Kevex

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} Does anyone in the group know the telephone number of the company
} called Kevex, (I may have spelled it wrong), this I believe is the company
} that manufactures EDS equipment. If I could have either the telephone number,

I use 1-800-495-3839. They list 805-295-0019 and Valencia, CA on the back
of their brochures.

Just a user. No endorsement implied.
----------------------------------------------------
Warren E. Straszheim
270 MD Ames Lab/ISU, Ames IA, 50011
Phone: 515-294-8187 FAX: 515-294-3091
E-Mail: wes-at-ameslab.gov (or: wesaia-at-iastate.edu)

coal characterization and processing
electron microscopy, x-ray analysis, image analysis
computer applications





From: Dwight Beebe :      beebed-at-ere.umontreal.ca
Date: Mon, 23 Oct 1995 16:18:24 -0400 (EDT)
Subject: LM: searching for dye

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Greetings,

I'd like to know whether anyone knows of a source for a dye
called Chlorazol Paper Brown (also identified as "Michrome No. 94"). The
stain is cited in Gurr's Encyclopedia of Microscopic Stains (1960), with
the original reference given as: Verdcourt, B., 1947, Chlorazol Paper
Brown B as a stain for plant tissue. Stain Tech 22:155-156. The dye is
a dis-azo series acid dye. I have been unable to find it in any of our
catalogs and would like to try it. If anyone can point me in the right
direction or, perhaps, provide me with a small amount, I would apreciate
it greatly.

Thanks in advance!

Dwight Beebe
Institut de recherche en biologie vegetale beebed-at-ere.umontreal.ca
Dept. de sciences biologiques Voice:514-872-4563
Universite de Montreal FAX:514-872-8496
4101, rue Sherbrooke est
Montreal, PQ H1X 2B2 Canada




From: Thomas F. Kelly :      TFKELLY-at-msae.engr.wisc.edu
Date: Mon, 23 Oct 1995 21:20:30 CST
Subject: Symposium on Instrumentation

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If it is appropriate, please post this on the Bulletin Board.
Thanks, Tom Kelly

Microscopists:
Please note the Symposium below.
****************************************************
CALL FOR PAPERS

INNOVATIONS IN INSTRUMENTATION FOR MATERIALS RESEARCH
Symposium AA at the 1996 Spring Meeting of the Materials Research Society
April 8 to 12, 1996, San Francisco, CA

We are in the midst of an explosion in new technologies for microscopy,
spectroscopy, data analysis, sample manipulation and more. The materials
community is central to much of this activity and both drives and embraces
these developments. The need for new instrumentation is especially poignant
now with the increasing need to understand materials on the atomic scale and
with greater precision in physical properties measurements. This symposium
will concentrate on the innovations and the people who develop the
innovations. Analytical characterization of materials including at least
imaging, elemental composition, chemical properties, and mechanical
testing on a microscopic scale will be considered.

To our knowledge, there has never been a symposium which specifically
seeks to bring together the developers of the instrumentation for the
purpose of reviewing the latest innovations in the technology. We expect
both the developers and users of instrumentation to be interested in this
symposium. Academic, industrial, and commercial groups developing
either systems or components which advance the state of instruments used
for materials research are invited to participate. We expect a
cross-fertilization among several communities. Examples of topics of
interest include:

1) System Components
a. Radiation Sources
b. Optics
c. Motion Technology, Stagework
d. Sensors and Detectors
e. Attenuation of Environmental Detriments

2) Integrated Systems
a. Microscopy and Spectroscopy
b. Microscopic Mechanical Test Systems

3) Enabling Instrumentation and Manufacturing
a. Microfabrication
b. Computer Hardware and Software

Invited speakers include: Ruud Tromp (IBM Watson Research Laboratory),
Pupa De Stasio (Swiss Institute of Technology), Noel MacDonald (Cornell
Univeristy), Ronald Gronsky (University of California - Berkeley), Ondrej
Krivanek (Gatan), Kamal Soni (University of Chicago), Jon McCarthy
(NORAN Intruments), Heiner Jaksch (Zeiss), David Williams (Lehigh),
Hans Hallen (North Carolina State University), Franco Cerrina (University
of Wisconsin), Michael Miller (Oak Ridge National Laboratory), Jan Ringnalda
(Philips), Warren Oliver (Nano Instruments Inc.), and more.

It is expected that some exceptional contributed papers will be upgraded
to longer, invited presentations.

Symposium Organizers:

Thomas F. Kelly
Dept. Materials Science & Eng.
University of Wisconsin
1500 Engineering Drive
Madison, WI 53706-1607
Phone: 608 263-1073
Fax: 608 263-1087
tfkelly-at-engr.wisc.edu

Paul E. Fischione
E. A. Fischione Instruments, Inc.
9003 Corporate Circle
Export, PA 15632
Phone: 412 325-5444
Fax: 412 325-5443
paul.fischione-at-internetmci.com

Lorretta Inglehart
National Science Foundation
Division of Materials Research
4201 Wilson Boulevard, 1065 S
Arlington, VA 22230
Phone: 703 306-1817
Fax: 703 306-0515
lingleha-at-nsf.gov

David B. Williams
Dept. Materials Science & Eng.
Lehigh University
Whitaker Lab #5,
5 E. Packer Ave.
Bethlehem, PA 18015-3195
Phone: 610-758-4224
Fax: 610 758-4244
dbw1-at-lehigh.edu

****************************************************
Abstract Model - 1996 Spring Meeting
Abstract Deadline: November 1, 1995
Submit Abstract to:
ATTN.: ABSTRACT ENCLOSED
Materials Research Society
9800 McKnight Road
Pittsburgh, PA 15237-6006
Fax: (412) 367-4373
Submitted to Symposium AA
Symposium Title: Innovations in Instrumentation for Materials Research
TITLE OF PAPER - ALL CAPITAL LETTERS
This set of instructions is given in the style and format to be used
by authors in preparing abstracts. Follow this example in preparing
your abstract. Reproduction will be done by photographing the abstract
copy exactly as received and reducing it in size by 33%. You may use
the special templates for this purpose obtainable from symposium organizers or


MRS Headquarters; our you may submit your abstract on white paper, in a
space no larger than 5 in. (12.7 cm) wide by 5 in. (12.7 cm) long. At the
top of the page above the text, you must include the name of the meeting and
symposium title; at the bottom, or on a separate page, list all authors
(full name, complete mailing address, phone, fax. e-mail).

Input Instructions

Type the title in capital letters, single space. Begin the text two
lines below the last line of the title: single space, with double spacing
between paragraphs.

Printing Guidelines

If you use a word processor or computer, print using a letter-quality
printer (e.g., impact or laser printer - do not use a dot matrix printer) or
use an electric typewriter with carbon ribbon.

Abstract Submission

Submit one camera-ready abstract to MRS Headquarters. Contributors
must observe established deadlines to ensure being listed in the Program book.



Contact Author:
MRS ID No. (if known)
Last Name
First Name
Institution
Department
Street/P.O.Box
City
State/Zip
Country
Telephone
FAX
E-mail

Presenting Author:
MRS ID No. (if known)
Last Name
First Name
Institution
Department
Street/P.O.Box
City
State/Zip
Country
Telephone
FAX
E-mail

Co-Author:
MRS ID No. (if known)
Last Name
First Name
Institution
Department
Street/P.O.Box
City
State/Zip
Country
Telephone
FAX
E-mail

Co-Author:
MRS ID No. (if known)
Last Name
First Name
Institution
Department
Street/P.O.Box
City
State/Zip
Country
Telephone
FAX
E-mail

Please check if appropriate:
 Duplicate of fax submitted earlier
 Corrected/revised copy of abstract submitted earlier
 Change in title/author
 Change in text
 MRS Log No._______________________________
 Poster Presentation preferred
Note: If you send your abstract via fax: please wait to receive an
acknowledgment letter with the assigned MRS Log No. Fill in the
MRS Log No. on the line above, and send your camera-ready
abstract to MRS.

MRS, please send:
Mark with X.
 A 1996 Spring Meeting Graduate Student Award Application to
___________________(graduate student and an author of above abstract)
 A 1996 Spring Meeting Symposium Aide Application to
___________________(graduate student)

Thomas F. Kelly Internet: tfkelly-at-engr.wisc.edu
University of Wisconsin Phone: 608 263-1073
1500 Johnson Drive Fax: 608 263-3704 or 3-1087
Madison, WI 53706-1687




From: trenkler-at-imec.be
Date: Tue, 24 Oct 1995 15:15:09 +0100
Subject: subscribe

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From: kelloes-at-emlab.cb.uga.edu
Date: Tue, 24 Oct 1995 12:09:00 +0000
Subject: "Gentleman Jim" Slam Freezer

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I need information on exactly how did the "Gentleman Jim" slam
freezer obtain its name. Some have thought it came from the champion
boxer, James Corbett, who was later recognized as "Gentleman Jim".
He bacame famous for his hard left hook. If anyone out there can
give me information on this, I would appreciate it. Thank you in
advance. Cathy Kelloes, UGA Central EM Lab, Athens, GA.




From: roy-at-bayou.uh.edu (Roy Christoffersen)
Date: Tue, 24 Oct 1995 08:47:16 -0500
Subject: EPMA:Probe standard for Co

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Does anyone know of a good microprobe standard for Co other than Co metal?
I have tried and failed to find alternatives, such as oxides, that can be
obtained in sufficiently large single crystals (i.e. } 10um). What about
Co-bearing silicate glasses? Silicides? Any help would be greatly
appreciated.


Roy Christoffersen
Texas Center for Superconductivity
3201 Cullen
Houston, TX 77204-5932
roy-at-bayou.uh.edu
(713) 743-8273
FAX: (713) 743-2787






From: mgarment-at-facstaff.wisc.edu (Martin B. Garment)
Date: Tue, 24 Oct 1995 13:20:41 -0500
Subject: Subscribe

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Subscribe Microscopy mgarment-at-facstaff.wisc.edu
Martin B. Garment
Ophthalmology and Visual Sciences
1300 University Ave. Rm. 6687
Madison WI 53706
Voice (608) 262-9596
Fax (608) 262-0479
Email - mgarment-at-facstaff.wisc.edu





From: Francisco J. Hernandez :      fjhblasq-at-biomed.icb2.usp.br
Date: Tue, 24 Oct 1995 15:11:10 -0300 (EST)
Subject: EM: Kodak film 5302 35 mm

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Could someone help us to find where we can buy
this film (Kodak film 5302 kodak 35 mm CAT Number 166 7229
Eastman Fine grain)?
Our EM (Philips) only works with 35 mm film and the best results we can
obtain is with this film, but we cannot find it in Brazil.
We want to import it using our credit card, by mail.

Thanks in advance

=============================================================================
Francisco Javier Hernandez Blazquez |
Universidade de Sao Paulo - Faculdade de | e-mail fjhblazq-at-spider.usp.br
Zootecnia e Engenharia de Alimentos | Voice: 55 195 616122 r. 265
Departamento de Ciencias Basicas/Histologia| r. 278
Av. Duque de Caxias Norte, 225 CP 23 | Fax: 55 195 611689
CEP 13630-000 Pirassununga (Sao Paulo) | 55 11 8191378
BRAZIL |
==============================================================================











From: M.V. Parthasarathy :      mvp2-at-cornell.edu
Date: Tue, 24 Oct 1995 14:48:12 -0400 (EDT)
Subject: RE: "Gentleman Jim" Slam Freezer

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X-NUPop-Charset: English

In message Tue, 24 Oct 1995 12:09:00 +0000, kelloes-at-emlab.cb.uga.edu writes:

} I need information on exactly how did the "Gentleman Jim" slam
} freezer obtain its name. Some have thought it came from the champion
} boxer, James Corbett, who was later recognized as "Gentleman Jim".
} He bacame famous for his hard left hook. If anyone out there can
} give me information on this, I would appreciate it. Thank you in
} advance. Cathy Kelloes, UGA Central EM Lab, Athens, GA.
----------------
My understanding is that the name came from the fact the specimen is not
just slammed against a cold metal block as in some devices (eg."Slammer")
that can produce repeated mini bounces. "Gentleman Jim" has a shock-absorbing
glycerine-filled device that absorbs the impact of slamming, prevents the
mini bounces and yet holds the specimen firmly against the cold block. That
is, it handles the specimen relatively "gently" but firmly!

*************************************************************************
M.V. Parthasarathy
Professor of Plant Biology &
Director, Cornell Integrated Microscopy Center
Section of Plant Biology
228 Plant Science Building
Cornell University, Ithaca, NY 14850
Telephone: (607) 255-1734
Fax: (607) 255-5407
E-mail: mvp2-at-cornell.edu
***********************************************************************




From: bozzola-at-siu.edu (John. J. Bozzola)
Date: Tue, 24 Oct 1995 16:03:11 -0600
Subject: TEM: Faraday cup for EDX

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X-Sender: bozzola-at-saluki-mail.fiber2.siu.edu
Message-Id: {v01510105acb30ebb7481-at-[131.230.97.69]}
Mime-Version: 1.0
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We need to measure the beam current in our variable pressure SEM to improve
our quantitative x-ray analysis using EDX. (Our SEM does not have a
specimen current meter, so we can not fabricate one as recently described
by S.D. Walck in Microscopy Today.) Does anyone make a simple, inexpensive
unit with a meter for measuring the current accurately? We could build one
if plans/specifications were made available.

#############################################################################
John J. Bozzola, Director
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
#############################################################################






From: hagler-at-utsw.swmed.edu (Herb Hagler, Ph.D.)
Date: Tue, 24 Oct 1995 16:20:29 -0500
Subject: Re: "Gentleman Jim" Slam Freezer

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Message-Id: {v01530504acb30842639d-at-[129.112.20.12]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

} I need information on exactly how did the "Gentleman Jim" slam
} freezer obtain its name. Some have thought it came from the champion
} boxer, James Corbett, who was later recognized as "Gentleman Jim".
} He bacame famous for his hard left hook. If anyone out there can
} give me information on this, I would appreciate it. Thank you in
} advance. Cathy Kelloes, UGA Central EM Lab, Athens, GA.


I remember asking Alan Boyne, the designer of the "Gentleman Jim" why he
named it that. As I remember, he told me that everyone was talking about
'slam freezing' which sounded like a very distructive way to freeze
biological tissue, hence his use of "Gengleman Jim", implying a very gentle
way to freeze tissue. It had nothing to do with the above referenced
fighter.

Herb Hagler, Ph.D.
Director of Computer-Assisted Instruction
for Southwestern Medical School
UT Southwestern Medical Center
(214)648-3890 fax(214)648-3925






From: ENERGY BEAM SCIENCES, INC :      75767.640-at-CompuServe.COM
Date: 24 Oct 95 15:28:01 EDT
Subject: Re: "Gentleman Jim" Slam Freezer

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Cathy Kelloes asks:
"I need information on exactly how did the "Gentleman Jim" slam
freezer obtain its name."
Well, it's like this...
The first commercial device to utilize the "slam freezing" technique was the
"Slammer" from BioRad. The problem with this device turned out to be that the
specimen would "bounce" against the freezing surface after the first impact,
distorting the morphology.
Alan Boyne, of the University of Maryland, Baltimore, figured out how to avoid
"bounce" by softening the impact of the specimen against the freezing surface (a
copper anvil) using a hydraulic mechanism. He introduced his new instrument at
a Cell Biology meeting in the mid-80's as "Gentleman Jim", the implication being
that, unlike the rough treatment of the specimen by the Slammer, the new
instrument would be gentle on the specimen. Immediately after the meeting,Alan
Boyne signed an exclusive distribution arrangement with Ted Pella, Inc., who
kept the name. The rest, as they say, is history.
Alan Boyne now lives in Friday Harbor, Washington, in a cabin or yurt or some
such thing which he built himself. By now, he may have a phone.
Steven Slap
P.S. The "Gentleman Jim" is still available through Energy Beam Sciences.





From: Jay Jerome :      jjerome-at-isnet.is.wfu.edu
Date: Tue, 24 Oct 1995 13:51:25 -0400 (EDT)
Subject: Re: "Gentleman Jim" Slam Freezer

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On Tue, 24 Oct 1995 kelloes-at-emlab.cb.uga.edu wrote:

} I need information on exactly how did the "Gentleman Jim" slam
} freezer obtain its name. Some have thought it came from the champion
} boxer, James Corbett, who was later recognized as "Gentleman Jim".
} He bacame famous for his hard left hook. If anyone out there can
} give me information on this, I would appreciate it. Thank you in
} advance. Cathy Kelloes, UGA Central EM Lab, Athens, GA.
}
I am not sure how much I can add to the discussion, but my recollection
from early conversations when the Gentleman Jim was first introduced was
that the name was chosen as a counterpoint to the term Slam Freezer,
emphasizing that the tissue was not damaged in the slamming process.


Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-isnet.is.wfu.edu *
**************************************************************





From: paulc-at-arms.gps.caltech.edu (Paul K. Carpenter)
Date: Tue, 24 Oct 1995 17:19:27 -0800
Subject: Re: EPMA:Probe standard for Co

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Message-ID: {dantus.1164957150B-at-slater.cem.msu.edu}

Roy Christoffersen asked,

} Does anyone know of a good microprobe standard for Co other than Co metal?
} I have tried and failed to find alternatives, such as oxides, that can be
} obtained in sufficiently large single crystals (i.e. } 10um). What about
} Co-bearing silicate glasses? Silicides? Any help would be greatly
} appreciated.
}
}
} Roy Christoffersen
} Texas Center for Superconductivity
} 3201 Cullen
} Houston, TX 77204-5932
} roy-at-bayou.uh.edu
} (713) 743-8273
} FAX: (713) 743-2787

Personally, I use a cobalt olivine (Co2SiO4) for most silicate analysis.
It was synthesized a number of years ago, and I'm afraid I don't know where
more material is located.

You might give Bart Cannon at Cannon Microprobe a call:

Cannon Microprobe
1041 NE 100th St.
Seattle WA 98125
206-522-9233

I see that he has several Co standards, of which a CoAl2O4 looks promising.
I have not yet purchased any material from Cannon, so I cannot vouch for
this or any other material.

Geller Microanalytical has two cobalt standards (Co3O4 and CoSi2) that
would be good candidates (their new number is 508-887-7000).

Finally, the so-called Corning glass standards that the Microbeam Analysis
Society used to distribute (and I am now by default the person in charge of
these materials) have nominally 0.8 % of a suite of elements in a CaMgSiB
glass. One of these glasses contains Co at about this concentration.
These glasses are useful as secondary standards, not for primary
calibration. They also suffer from a lack of complete agreement on the
compositions from various techniques.

I have no connection with either of these commercial sources.

Good luck,

Paul Carpenter


+---------------------------------------------------------------------------+
| Paul K. Carpenter paulc-at-arms.gps.caltech.edu |
| Division Analytical Facility Department of Geology 170-25 |
| California Institute of Technology Pasadena CA 91125 |
| 818-395-6126 (X-ray Lab) 818-568-0935 (FAX, Departmental) |
+---------------------------------------------------------------------------+






From: GVKM07A-at-PRODIGY.COM (DR CHARLES A GARBER)
Date: Tue, 24 Oct 1995 21:14:09 EDT
Subject: "Best" microtome

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-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --

For some years, I have had the opportunity to visit EM laboratories
virtually all over the world, and believe it or not, the subject of
which is the "best microtome" seems to come up even more often than
which is the "best electron microscope". Perhaps I am asked questions
like that because we have absolutely no vested interest in our answer
and people know that.

And I have come away from such conversations realizing that the single
strongest factor in customer satisfaction in terms of microtomes if not
most things is based on the quality of local service. I stress the
word "local" because the quality of the service in one city or country
has no relationship (from what I can see) with the level of service in
some other location in the world (or even how close or far one might be
located relative to the factory making the microtome). Some times the
high quality service does not come from the manufacturer at all, but
from a "third party" service organization, and in some instances, they
will service any brand of ultramicrotome.

To evaluate the quality of the local service that is available, instead
of going to the biggest name person and getting their opinion, go to
the laboratory that runs the highest volume of samples. Find out how
much down time they experience. If the down time is relatively low,
then they are getting good service. You don't even have to ask about
the quality of the sections, the two major manufacturers both make
excellent equipment. But "happiness" with an ultramicrotome is
synonomous with how happy one is with their local service provider.

Chuck

======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail:
GVKM07A-at-prodigy.com
West Chester, PA 19381-0656 USA Customer Service:
SpiSupp-at-aol.com

########################################################
WWW: http://mail.cccbi.chester.pa.us/spi/spihome.html
########################################################
======================================================





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Date: Wed, 25 Oct 1995 08:23:08 -0400
Subject: unsubscribe

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From: Carl Henderson :      chender-at-umich.edu
Date: Wed, 25 Oct 1995 10:06:05 -0400 (EDT)
Subject: Re: EPMA:Probe standard for Co

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On Tue, 24 Oct 1995, Roy Christoffersen wrote:

} Does anyone know of a good microprobe standard for Co other than Co metal?

We use a Co-sulfide with success. Unfortunately I do not know the source
or do we have any extra!

Carl

Carl Henderson
Electron Microbeam Analysis Laboratory
University of Michigan
2005 C.C. Little Bldg.
Ann Arbor, MI 48109-1063 USA
--------------------------------
(313) 936-1550 (voice)
(313) 763-4690 (FAX)
chender-at-umich.edu (e-mail)
--------------------------------




From: Mount, Richard :      MOUNTR-at-hermes.is.sickkids.on.ca
Date: Wed, 25 Oct 1995 10:21:00 -0500
Subject: Narishige Instruments

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Message-Id: {Megw.157505-at-hermes}


{Text_1}
Hi Everyone,

Does anyone know the address or phone # of a North American
agent for Narishige Instruments? We have a motor driven
micromanipulator which we bought several years ago that now
needs service. Unfortunately the contact number we had in
Japan is out of service.

Many thanks

Richard

------------------------------------------------------------
Richard J. Mount Phone: 416-813-6551
Auditory Science Laboratory
Department of Otolaryngology FAX: 416-813-5036 The
Hospital for Sick Children
555 University Ave.
Toronto, ON, Canada M5G 1X8

e-mail:richard.mount-at-mailhub.sickkids.on.ca




From: tphillips-at-biosci.mbp.missouri.edu (Tom Phillips)
Date: Wed, 25 Oct 1995 09:12:04 -0600
Subject: Re: "Gentleman Jim" Slam Freezer

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As Alan Boyne's graduate student and one who was intimately involved with
the Gentleman Jim's development, I would like to confirm that Alan named
the Gentleman Jim as a subtle dig at the "slammer" which was the principal
alternative at the time. He was, of course, well familar with the famous
boxer of the same name. Alan is living on Friday Harbor and his phone
number is 206-378-7252. If you want any more details on the Gentleman Jim
feel free to contact Alan or myself. By the way, I don't think Alan has a
distributor anymore for the Gentleman Jim but I think he would be willing
to provide one for anyone who is in the market.

Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility
3 Tucker Hall
University of Missouri
Columbia, MO 65211
(314)-882-4712 (voice)
(314)-882-0123 (fax)






From: gwe-at-biotech.ufl.edu (Greg Erdos)
Date: Wed, 25 Oct 1995 14:47:59 GMT
Subject: Zamboni's fixative

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I can recall, that as a callow youth we used Zamboni' fixative. What I do
not recall is its composition.

If there any one out there who can supplement my memory, I would appreciate
the recipe.
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Greg Erdos Phone: 904-392-1295
Scientific Director,
ICBR Electron Microscopy Core Lab
218 Carr Hall Fax 904-846-0251
University of Florida E-mail: gwe-at-biotech.ufl.edu
Gainesville, FL 32611
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-





From: kaurin-at-rmslab.rockefeller.edu
Date: Wed, 25 Oct 1995 16:41:57 EDT
Subject: Many thanks/Best Microtome

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Thanks to all who replied to my query. It was wonderful to hear from
you all and to benefit from your experience.
Shelley Landon Kaurin






From: Mount, Richard :      MOUNTR-at-hermes.is.sickkids.on.ca
Date: Wed, 25 Oct 1995 11:03:00 -0500
Subject: Re: SEM to TEM

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Message-Id: {Megw.157544-at-hermes}


{Text_1}
On Oct. 22, Felicity Lawrence Inquired about marking corneal
SEM specimens for location during TEM.
Many years ago we had the same problem using cochlear
specimens. Our best results were achieved by bolting a
micromanipulator (de Fonbrune type, pneumatic) into one of
the ports of a JEOL JSM35 SEM. Although making 3D
manipulations while observing a 2D image was difficult we
were able to scratch delimiting marks around areas of
interest. These marks could be seen in the embedded
specimen and, if cutting at right angles to the surface, in
thick sections. The procedure was only marginally
successful on gold coated specimens as the scratched area
would charge, however, using the O-T-O-T-O technique we were
very successful. This technique has the added advantage
that sections cut for TEM do not need staining as there is
sufficient osmium to provide contrast.

Hope this info. is of some help, best of luck,

Richard

------------------------------------------------------------
Richard J. Mount Phone: 416-813-6551
Auditory Science Laboratory
Department of Otolaryngology FAX: 416-813-5036 The
Hospital for Sick Children
555 University Ave.
Toronto, ON, Canada M5G 1X8

e-mail:richard.mount-at-mailhub.sickkids.on.ca




From: MacisNo1-at-aol.com
Date: Wed, 25 Oct 1995 17:19:55 -0400
Subject: Narishige, USA

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The information you requested is as follows...

Narishige USA, Inc.
404 Glen Cove Avenue
Sea Cliff, NY 11579

(516) 676-0044
(800) 445-7915
(516) 676-1480 FAX

Good Luck,
Lawrence Kordon
MacisNo1-at-aol.com
Nikon, Inc.




From: Gardnerjs-at-yvax.byu.edu (flavio ortolano gardnerjs)
Date: Wed, 25 Oct 1995 14:56:12 -0300
Subject: Durst Lenses (Reduction)

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Hi all:

We have a need for reduction lenses for our Durst Enlarger. Could anyone
send me names, addresses, and/or phone numbers of resources.

Thanks for your help. I look forward to hearing from you.

John Gardner
801-378-2202






From: Carlos E. Barbosa :      grial-at-relay.starnet.net.ar
Date: 10/25/95
Subject: LM listserver

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From: paulc-at-arms.gps.caltech.edu (Paul K. Carpenter)
Date: Wed, 25 Oct 1995 11:34:14 -0800
Subject: Re: EPMA:Probe standard for Co

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Message-Id: {v02110101acb43e9b6f14-at-[131.215.67.110]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Roy Christoffersen asked,

} Does anyone know of a good microprobe standard for Co other than Co metal?

Actually, Co metal is pretty good for a cobalt standard. The mass
absorption coefficients for Co Ka in an oxygen and sulfur matrix are:

Co Ka by Oxygen, mac 17.5
Co Ka by Sulfur, mac 143.7

Co Ka is a hard x-ray line such that it doesn't really matter what the
matrix is for light elements like this. However, if you are looking at Co
in an Fe matrix, the mac is much higher, since Fe Ka is fluoresced by Co Ka
(and hence Co Ka is absorbed):

Co Ka by Fe, mac 401.9

So if you need to analyze Co in stainless steel or Fe-bearing spinel, then
it may be a good idea to find a well-characterized Fe-bearing Co standard.
But in general (for silicate systems with low Fe), the absorption
correction is going to be fairly small, and if Co is a trace element, the
dominant problem is just getting some x-ray counts to begin with.

Now light element measurement, that is where standards must be carefully chosen.

Paul


+---------------------------------------------------------------------------+
| Paul K. Carpenter paulc-at-arms.gps.caltech.edu |
| Division Analytical Facility Department of Geology 170-25 |
| California Institute of Technology Pasadena CA 91125 |
| 818-395-6126 (X-ray Lab) 818-568-0935 (FAX, Departmental) |
+---------------------------------------------------------------------------+






From: A. Kent Christensen :      akc-at-umich.edu
Date: Wed, 25 Oct 1995 17:50:23 -0400 (EDT)
Subject: Re: Zamboni's fixative

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Greg,

The main reference is: Stefanini et al., 1967, Nature 216:173.

Kent
A. Kent Christensen, Univ. of Michigan, {akc-at-umich.edu}

----------------------------------

On Wed, 25 Oct 1995, Greg Erdos wrote:

} I can recall, that as a callow youth we used Zamboni' fixative. What I do
} not recall is its composition.
}
} If there any one out there who can supplement my memory, I would appreciate
} the recipe.
} -at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
} Greg Erdos Phone: 904-392-1295
} Scientific Director,
} ICBR Electron Microscopy Core Lab
} 218 Carr Hall Fax 904-846-0251
} University of Florida E-mail: gwe-at-biotech.ufl.edu
} Gainesville, FL 32611
} -at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
}
}




From: Jinghua Tang :      jinghua-at-pro.sb.fsu.edu
Date: Wed, 25 Oct 1995 23:19:41 -0400
Subject: Re: Zamboni's fixative

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Hi Everyone,

Does anyone have any ideas on how to combine data
from Electron microscopy reconstruction and X-ray
crystallography in image analysis when the complex
structure obtained from EM and some components'
structure came from CRYSTALLOGRAPHY?

Many thanks!

Jinghua

--



$&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&$
$ Institute of Molecular Biophysics 206 Rogers Hall,Ubox 65455 $
$ Florida State University Florida State University $
$ Tallahassee, FL 32306-3015 Tallahassee, FL 32323 $
$ (904) 644-1115 (904) 853-1498 $
$ jinghua-at-sb.fsu.edu http://www.sb.fsu.edu/~jinghua $
$&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&$




From: Stephen Edgar :      s.edgar-at-auckland.ac.nz
Date: Thu, 26 Oct 1995 18:13:59 NZS
Subject: Keeping absolute ethanol dry (for biological EM)

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Due to space restrictions (yes, even in a practically empty country
like New Zealand!) we have had to dismantle the still with which I
used to produce our own super-dry ethanol.

From now on we will be using commercial absolute ethanol for EM
specimen dehydrations, and I am seeking advice on what to put in the
opened containers of ethanol to absorb any traces of water. I never
cared much for molecular sieve pellets because of the dust that comes
off them.

Any suggestions on what to use, the quantity required and its
"lifespan" in use, and how to package the desiccant within the
bottles, will be gratefully received and a summary posted to the list.


Regards

Stephen Edgar

Electron Microscope Unit, Pathology Department
School of Medicine
University of Auckland
Private Bag 92019
Auckland
New Zealand

email address: s.edgar-at-auckland.ac.nz
Phone : +64-9-3737599 extn 6473 (GMT + 12h)
Fax : +64-9-3737459




From: GVKM07A-at-PRODIGY.COM (DR CHARLES A GARBER)
Date: Thu, 26 Oct 1995 00:32:42 EDT
Subject: KODAK 5302 35 mm film

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-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --

On Oct. 24, F.J. Hernandez asked about where he could purchase Kodak
5302 35 mm roll film.

Actually, this product is available from most of the major suppliers of
EM consumables, such as SPI Supplies.

Naturally I am a bit biased in terms of which one you should consider
first, but just about all of the major EM consumables suppliers handle
this particular Kodak product. With the heightened security
precautions in effect worldwide, make sure you ask your vendor to place
special "do not x-ray" stickers on the boxes, other wise you will have
rather foggy micrographs, which I am sure you don't want to have. You
also have to realize that even with the "do not x-ray" stickers, there
is still the risk that the box will be x-rayed and the risk of that
happening is on the customer, not the supplier.

At the present time, we advise NOT to send your VISA card number and
expiration date over the internet. That is what the experts say. You
should send it by FAX which is more secure. When you do that the first
time, with SPI, you can also give authorization to use the same card in
the future by special authorization code, that is in the future, for
additional orders, you can just refer to your VISA card number on file
via the internet but without actually "giving" your number again.


Chuck

======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail:
GVKM07A-at-prodigy.com
West Chester, PA 19381-0656 USA Customer Service:
SpiSupp-at-aol.com

########################################################
WWW: http://mail.cccbi.chester.pa.us/spi/spihome.html
########################################################
======================================================





From: wesleysm-at-biology.und.ac.za (James Wesley-Smith)
Date: Thu, 26 Oct 1995 10:12:05 +0200
Subject: Re: Durst Lenses (Reduction)

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Comments: Authenticated sender is {wesleysm-at-biology.und.ac.za}
Gardnerjs-at-yvax.byu.edu (flavio ortolano gardnerjs)

Dear John
I have a Durst M670 enlarger and I use a combination of the VEGATUB 39
lens board with a 50 mm lens to make transparencies of 6x7 cm
negatives. (Note: The condenser must match the negative size.) The
enlarger is racked down to about 10 cm from the easel, and by manipulating
the racking and focussing controls you will be able to vary the size of the
final (focussed) image.

What is actually taking place by doing this is that the object
(negative) is placed outside twice the focal length of the lens, resulting in
demagnification. If you require something intermediate between a
large format and a 35mm size, I suggest using an 80mm lens.

I hope this helps you.

James Wesley-Smith
Electron Microscope Unit
George Campbell Building
University of Natal
Durban, South Africa





From: Stephan Coetzee :      STEPHAN-at-gecko.biol.wits.ac.za
Date: Thu, 26 Oct 1995 11:17:41 GMT+2
Subject: Fixing bees

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Dear Microscopists

A student here wants to fix and store bee's for a prolonged period of
time. Any suggestions as to stop at primary fixation or after
dehydration. Which buffer is preferrable i.e. Na-cacodylate or
Phosphate buffer.

Thanks

Stephan H Coetee
Electron Microscope Unit
Private Bag 3
Wits
2050 Stephan-at-Gecko.biol.WITS.ac.za

Tell: (011) 716 2419
Fax : (011) 339 3407




From: bjg-at-uniwa.uwa.edu.au (Brendon J. Griffin)
Date: Thu, 26 Oct 1995 16:38:38 +0800
Subject: NIH image version for EXECUTOR

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Message-Id: {199510260837.QAA07465-at-uniwa.uwa.edu.au}

Goodai

Could anyone advise me which (MAC) NIH IMAGE version is compatible with the
IBM -MAC emulator 'executor'. I have tried v1.57 and 1.47 and get an error
message relating to a lack of qiuckdraw with v1.57 and v1.47 just crashes

Thank you


Brendon
Brendon J. Griffin
Centre for Microscopy and Microanalysis
The University of Western Australia
Nedlands, WA, AUSTRALIA 6907
ph 61-9-380-2739 fax 61-9-380-1087





From: ENERGY BEAM SCIENCES, INC :      75767.640-at-CompuServe.COM
Date: 26 Oct 95 08:19:26 EDT
Subject: Re: EPMA:Probe standard for Co

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Message-ID: {MAPI.Id.0016.00683536202020203832384530303034-at-MAPI.to.RFC822}
Priority: Normal
To: LISTSERVER {microscopy-at-Sparc5.Microscopy.Com}
MIME-Version: 1.0

For those of you interested in experimenting, I have found a source of foils
0.025mm thick of the following cobalt compounds:
C069/B12/Si12/Fe4/Mo2/Nil
Co66/Si16/B12/Fe4/Mo2
Co66/Si15/B14/Fe4/Nil
Steven Slap, Vice-President
Energy Beam Sciences, Inc.





From: mgeorge-at-dubois.fisk.edu (Michael George)
Date: Thu, 26 Oct 1995 08:26:40 -0500
Subject: Unsubscribe

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Un subscribe Michael George, mgeorge-at-dubois.fisk.edu


Michael A. George, Ph.D.
*******************************************
Research Assistant Professor
Department of Physics
Fisk University
Nashville, TN 37208

Office: (615)329-8525 Lab: (615)329-8736
Fax: (615)329-8634 mgeorge-at-dubois.fisk.edu





From: s.griffiths-at-ucl.ac.uk (Stephen Griffiths)
Date: Thu, 26 Oct 1995 15:12:44 +0100
Subject: LM: Biocytin - Query Bad batch?

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Message-Id: {9510261119.AA03724-at-carbon60.fysik.dtu.dk}

A colleague of mine is using Biocytin for neural tracing in brain.

He has had problems with one batch of Biocytin not producing any label,
whilst another older one, now used up, from the same company is OK.

He has asked if I would enquire of fellow Microscopy List members if they
have had any problems.

The Suspect Batch is:-
Sigma Chemical Co.
Biocytin - (Freebase)
Cat. No. #B4261
Batch No. 25H5851.

There is, apparently, only this one batch number available in the UK at the
moment from Sigma.

Sigma Biocytin is usually very reliable. Has anyone had any problem using
this particular batch?

Does anyone know of any other reliable source of Biocytin available in the UK?
If so could you let me know.

TIA.

Regards

Stephen Griffiths.


{} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {}
{} Stephen Griffiths {} e-mail s.griffiths-at-ucl.ac.uk {}
{} Visual Science Department {} {}
{} Institute of Ophthalmology {} Tel: 0171 608 6914 {}
{} London. EC1V 9EL. UK. {} Fax: 0171 608 6850 {}
{} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {}





From: kennedy-at-nsi.edu (grace kennedy)
Date: Thu, 26 Oct 1995 09:00:46 -0800
Subject: Narishige service

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X-Sender: grace-at-popmail
Message-Id: {acb56da70102100483ce-at-[198.147.151.19]}
Mime-Version: 1.0
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Since we're on the subject, I'd be interested to know if anyone has
actually had any micromanipulators rebuilt or repaired by Narishige-I know
someone here in Souther California that works on Narishige electronics, but
never have found anyone to do manipulators. Incidentally, Narishige was
useless in finding parts or repairs for the instrument I needed fixed
(vertical micropipette puller)-I just got lucky by making a lot of phone
calls. Grace Kennedy






From: Rolf Odselius :      Rolf.Odselius-at-emu.lu.se
Date: Thu, 26 Oct 1995 11:15:03 +0100
Subject: Re: Spurious Mo peaks

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} From: USERHHXS-at-um.cc.umich.edu
} Date: Wed, 25 Oct 95 12:03:20 EDT
} To: microscopy-at-Sparc5.Microscopy.Com
} Subject: Spurious Mo peaks

} We're getting large Mo peaks (and nothing else) from our Be window
} EDS detector, even without a sample holder in place. Another
} point of note is that when a bulk copper sample is inserted into
} the beam path, the same Mo peaks appear along with an additional
} pair of peaks a few eV lower in energy (these do not correspond
} to any realistic elemental peaks. Any thoughts as to the origin
} of these peaks. As far as I know, the only Mo on the column
} would be the aperture strips.

To understand your problem, it would be of help to know if the
microscope is a TEM or a SEM, telling something about the geometry
of the detector and samble holder in the specimen stage.

At which energies do the "false" peaks occur? One of them is probably
the Mo escape peak.

When you insert the copper specimen, do you see the copper peaks
along with the molybdenium peaks? If not, your collimator is probably
misaligned (out of position), and/or the specimen holder is not
properly aligned with the detector. The detector "sees" something
else than the sample. This something is most certainly made of
molybdenium. Detectors are usually installed so the sample is in the
center of the detector=B4s "visual field" when the sample altitude is
adjusted to the eucentric point of the goniometer.

Is the detector close enough to the sample? If not, the acceptance
angle of the collimator allows the detector to "see" to much of the
surroundings of the sample, e.g. the mechanisms of the sample holder.

Good luck!

Rolf Odselius
________________________________________________________________________
Rolf Odselius, PhD, Ass Prof
Electron Microscopy Unit, University Hospital
S-221 85 Lund, Sweden
Phone: +46 46 171075 Fax: +46 46 172975 Mobile phone: +46 10 6705655
Pager (Minicall): +46 740 288992 E-mail: Rolf.Odselius-at-emu.lu.se
URL: http://www.medfak.lu.se/medinst/emu/
Educational Secretary, SCANDEM URL: http://www.ldc.lu.se/~scandem/




From: zaluzec-at-Microscopy.Com (Nestor J. Zaluzec)
Date: Thu, 26 Oct 1995 14:02:15 -0500
Subject: David Hendriks & SBT

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South Bay Technology & David Hendriks can be reached by email at:

SBT-at-MSA.Microscopy.Com

or directly at

73531.1344-at-compuserve.com



Nestor






From: zaluzec-at-Microscopy.Com (Nestor J. Zaluzec)
Date: Thu, 26 Oct 1995 14:02:15 -0500
Subject: David Hendriks & SBT

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South Bay Technology & David Hendriks can be reached by email at:

SBT-at-MSA.Microscopy.Com

or directly at

73531.1344-at-compuserve.com



Nestor






From: STEELE-at-KRDC.INT.ALCAN.CA
Date: Thu, 26 Oct 1995 09:34:11 -0400 (EDT)
Subject: TEM prep: Epol X-sections

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--Boundary (ID PACtFaIgLPtJuQ00ZMEzQg)
Content-type: TEXT/PLAIN

Good Morning All,

I'm wondering if anyone can suggest a simple method for preparing cross
sections of thin ( {1mm) metal sheet by electropolishing.

I routinely do this using the tried and true(?) wafer stack, dimple, ion mill
method, but would like to find a quicker, Epol based method if possible.

I'm ruling out tripod polishing - ion milling for now (though I haven't tried
it yet) on the basis of the time (I think is) required.

My ideas run along the "jam" an ultrasonically cut stack into a 3mm tube and
hope for electrical contact route, but I'd appreciate some other ideas.

I recall seeing a poster once where a dimpling apparatus had been modified to
electopolish or at least chemically polish while dimpling. Did that line of
work ever continue?

I have have some colleagues who'll be interested, so please respond to the
server if you have any ideas on the matter.

Thanks in advance,

###############################################################
# #
# Don Steele STEELE-at-KRDC.INT.ALCAN.CA #
# ALCAN INTERNATIONAL #
# Kingston Research and Development Center #
# P. O. Box 8400 #
# Kingston, Ontario Canada K7L 5L9 #
# #
###############################################################



--Boundary (ID PACtFaIgLPtJuQ00ZMEzQg)--




From: baskin-at-biosci.mbp.missouri.edu (Tobias Baskin)
Date: Thu, 26 Oct 1995 08:48:52 -0500
Subject: Re: Keeping absolute ethanol dry (for biological EM)

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Message-Id: {v01520d00acb53f2c2c55-at-[128.206.15.200]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Greetings,

Stephen Edgar {s.edgar-at-auckland.ac.nz} wrote:
}
} } From now on we will be using commercial absolute ethanol for EM
} specimen dehydrations, and I am seeking advice on what to put in the
} opened containers of ethanol to absorb any traces of water. I never
} cared much for molecular sieve pellets because of the dust that comes
} off them.
} Any suggestions on what to use, the quantity required and its
} "lifespan" in use, and how to package the desiccant within the
} bottles, will be gratefully received and a summary posted to the list.

I have used molecular sieve encased in dialysis tubing, which seems
to have prevented any obvious dust problems. But unless the sieves
themselves are scrupulously dry, they can acutally ADD water to your
solvent. For freeze substitution work, I have had nice results with adding
1% acidified dimethoxypropane to substitution solvents (ethanol or
acetone). This compound reacts chemically with water to produce acetone and
methanol. Of course, I don't know if the benefits in freeze substitution
come from simply having more strictly anhydrous reagents, or if the dmp
actually speeds up substitution of the tissue. I should also mention, that
most of my work is at the light micrscope level, so I also can't comment
about ultrastructure. But I like the concept of removing water chemically,
with dmp, as opposed to physically with a sieve.
Hope this helps,
Tobias Baskin


- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
___ ____ ^ ____ _____ Tobias I. Baskin
/ \ / / \ / \ / University of Missouri
/ | / / \ / / Biological Sciences
/___ / /__ /_____\ / /__ 109 Tucker Hall
/ / / \ ( / Columbia, MO 65211 USA
/ / / \ \ / voice: 314-882-0173
/ /____ / \ \____/ /_____ fax: 314-882-0123






From: bozzola-at-siu.edu (John. J. Bozzola)
Date: Thu, 26 Oct 1995 15:47:45 -0600
Subject: SEM: Lion S vacuum pump oil

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A friend has a Hitachi S570 SEM that uses Lion-S (Japanese company)
diffusion pump oil and needs to replace some of the oil in his 500W dp's.
Anyone know of a vendor in the US? Thank you very much.

#############################################################################
John J. Bozzola, Director
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
#############################################################################






From: rsartore-at-arl.mil (Sartore, Richard G.)
Date: Thu, 26 Oct 1995 17:14 -0500 (EST)
Subject: Etch Platinum on GaAs

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Message-ID: {MAPI.Id.0016.00683536202020203143364230303042-at-MAPI.to.RFC822}
Read-Receipt-To: jean-louis beek {ah56-at-solo.pipex.co.za}
Priority: Normal
To: microscopy-at-Sparc5.Microscopy.Com
MIME-Version: 1.0

Would like to know if there is any way to selectively etch platinum on a
GaAs substrate.
Has anyone tried this or are they currently doing this???

I understand that aqua regia would remove platinum but have been advised
that it also
attacks GaAs. Are there any solutions that would slow down the rate of
attack on GaAS and
retain effectiveness on platinum???

Rich Sartore
Army Research Laboratory
AMSRL-PS-DC
Fort Monmouth, NJ 07703
rsartore-at-arl.mil
908-427-2261






From: NJWS-at-aol.com
Date: Thu, 26 Oct 1995 20:24:45 -0400
Subject: Re: KODAK 5302 35 mm film

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Some of us,although we would love to get a little free advertising on the
net,are absolutely scrupulous about not mentioning our company names,etc on
this listserver.We have been told that this is the rule and we are happy to
abide by this because we enjoy using this medium.

I think this latest communication by Doctor Garber not only advertises his
website catalog but actually gives ordering information for his company.This
may be denied under the guise of aiding subscribers but this and other
instances have in fact been advertising.

Just tell us if the rules have been changed or how we think the rule should
be interpreted so that the playing field can be level for all

Thanks,
Norm




From: MicroToday-at-aol.com
Date: Thu, 26 Oct 1995 19:00:31 -0400
Subject: Laser Printers

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Group-
Regarding inquiries in the recent past concerning laser printers:
After considerable survey, I recently purchased a Lexmark Optra R laser
printer - rated at 1200 dpi. The current price of the unit at "Best Buy" (a
discount house) is $1,299.50.
The unit, with 2K RAM, prints at 1200 dpi up to a certain, undefined
"density" per page - and, when exceeded, reverts back to 600 dpi. If one
always wants 1200 dpi, they recommend that you purchase an additional 8K RAM.
I also understand that one of the computer magazines rated the unit as their
"Best Buy."
I am truly delighted with the printer. The print quality, as one would
expect, is nifty. However, the ease of use and flexibily are equally
outstanding.
I recommend the unit very highly.
In passing, should you be interested in quality, printed reproduction of
images (as I am in our newsletter "Microscopy Today"), you may care to
consider the front cover image on our last (October) issue. It is the first
time that we used a (writeable) CD for input. Rather nice, yes?
Regards to all,
Don Grimes, Microscopy Today




From: Carl Henderson :      chender-at-umich.edu
Date: Thu, 26 Oct 1995 21:57:57 -0400 (EDT)
Subject: Re: SEM: Lion S vacuum pump oil

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On Thu, 26 Oct 1995, John. J. Bozzola wrote:

} A friend has a Hitachi S570 SEM that uses Lion-S (Japanese company)
} diffusion pump oil and needs to replace some of the oil in his 500W dp's.
} Anyone know of a vendor in the US? Thank you very much.
}

I substituted Octoil-S for Lyon-S a few years ago in our S570. Since
then, however, I switched to a larger heater (700W) and changed over to
Santovac 5 in an attempt to minimize oil contamination in the chamber.
(BTW, that did not solve the problem...)

Carl

Carl Henderson
Electron Microbeam Analysis Laboratory
University of Michigan
2005 C.C. Little Bldg.
Ann Arbor, MI 48109-1063 USA
--------------------------------
(313) 936-1550 (voice)
(313) 763-4690 (FAX)
chender-at-umich.edu (e-mail)
--------------------------------




From: m.dickson-at-unsw.edu.au (melvyn dickson)
Date: Fri, 27 Oct 1995 10:30:17
Subject: Re: Keeping absolute ethanol dry (for biological EM)

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} } From now on we will be using commercial absolute ethanol for EM
} specimen dehydrations, and I am seeking advice on what to put in the
} opened containers of ethanol to absorb any traces of water. I never
} cared much for molecular sieve pellets because of the dust that comes
} off them.

} Any suggestions on what to use, the quantity required and its
} "lifespan" in use, and how to package the desiccant within the
} bottles, will be gratefully received and a summary posted to the list.


} Regards

} Stephen Edgar

We use commercial absolute ethanol and acetone. We keep both dry with
dehydrated copper sulphate, which is made by baking cuso4.5h20 in a
crucible over a bunsen flame until the salt becomes a white powder. When its
cool, we put it in a "sausage skin" of dialysis tubing (say 10 mm diam.),
opened out and with a knot tied at one end. We tie off the other end and put
the cus04 sausage in the solvent. Unless you actually put water into the
bottle, it seems never to need changing. It is self indicating in that the
cuso4 goes blue again in the presence of water.

Mel dickson




From: baskin-at-biosci.mbp.missouri.edu (Tobias Baskin)
Date: Thu, 26 Oct 1995 17:39:58 -0500
Subject: Re: Keeping absolute ethanol dry (for biological EM)

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Message-Id: {v01520d06acb5be991c79-at-[128.206.15.200]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Greetings,
(Sorry if you get this twice--I never got the first one...)

Stephen Edgar {s.edgar-at-auckland.ac.nz} wrote:

} } From now on we will be using commercial absolute ethanol for EM
} specimen dehydrations, and I am seeking advice on what to put in the
} opened containers of ethanol to absorb any traces of water. I never
} cared much for molecular sieve pellets because of the dust that comes
} off them.
} Any suggestions on what to use, the quantity required and its
} "lifespan" in use, and how to package the desiccant within the
} bottles, will be gratefully received and a summary posted to the list.

I have used molecular sieve encased in dialysis tubing, which seems
to have prevented any obvious dust problems. But unless the sieves
themselves are scrupulously dry, they can acutally ADD water to your
solvent. For freeze substitution work, I have had nice results with adding
1% acidified dimethoxypropane to substitution solvents (ethanol or
acetone). This compound reacts chemically with water to produce acetone and
methanol. Of course, I don't know if the benefits in freeze substitution
come from simply having more strictly anhydrous reagents, or if the dmp
actually speeds up substitution of the tissue. I should also mention, that
most of my work is at the light micrscope level, so I also can't comment
about ultrastructure. But I like the concept of removing water chemically,
with dmp, as opposed to physically with a sieve.
Hope this helps,
Tobias Baskin


- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
___ ____ ^ ____ _____ Tobias I. Baskin
/ \ / / \ / \ / University of Missouri
/ | / / \ / / Biological Sciences
/___ / /__ /_____\ / /__ 109 Tucker Hall
/ / / \ ( / Columbia, MO 65211 USA
/ / / \ \ / voice: 314-882-0173
/ /____ / \ \____/ /_____ fax: 314-882-0123






From: ENERGY BEAM SCIENCES, INC :      75767.640-at-CompuServe.COM
Date: 27 Oct 95 08:02:45 EDT
Subject: Re: micromanipulators

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Message-ID: {n1397388085.29003-at-mse.engin.umich.edu}
Microscopy Listserver {Microscopy-at-Sparc5.Microscopy.Com}

Scott Walck asked about the de Fonbrune micromanipulators. They are made in the
United States by Technical Products International. You should contact Ralph
Willen at TPI at 314-522-8671 (phone) or 314-522-6360 (fax) for more
information.
Steven Slap, Vice-President





From: orion-at-infoboard.be (Jean Leclef)
Date: Fri, 27 Oct 1995 13:37:23 +0100
Subject: Laser printing

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Message-Id: {199510271238.HAA04261-at-Sparc5.Microscopy.Com}


for those who are intested in laser printing

the following company in New York has a very good alternative to print
images on Laserjet 3/4/4+
printers - it is a German interface board with 150, 300 and even 600 dpi
printing capability, with a palette of 256 from 1024 gray levels. it works
really fine for printing hires SEM images and prints a 2 MByte image in seconds!

the experience shows that it is a real alternative to photographic images
when you have to find another way to print good pictures from a SEM/STEM.

contact :
Electro Image, Inc.
9, Round Hill Road
LAKE SUCCESS, NEW YORK 11020
tel 516 773 4305
fax 516 773 2955


Jean Louis Leclef





From: smithj-at-acad.winthrop.edu
Date: Fri, 27 Oct 1995 11:07:39 -0400
Subject: ?SEM of Plant Chromosomes? How-to?

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Microscopists:
A student and I are trying to do SEM of wheat chromosomes (with the
ultimate goal of gold-labelled in-situ's). We have the nice chapter
by Harrison and Jack in Hayat's "Correlative Microscopy", and the
Scheid and Tarut citation therein on Vicia faba. Anyone out there
have anything more recent to point us to?
TIA
Julian Smith III
Biology




From: MicroToday-at-aol.com on Thu, Oct 26, 1995 10:56 PM
Date: 27 Oct 1995 11:16:11 -0500
Subject: Laser Printers

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Message-Id: {n1397329007.52636-at-msmail.tmc.tulane.edu}

Don, how are you? The most importante piece of information is missing from
yours and other's answers!. How long it takes to print a 8x10 page at
1200dip? I received a flyer from Nat. Graphic Supply (800-223-7130)
advertising a 1200x1200 printer name XANTE (no price given), but do not know
anything about it. Regards, Cesar.
_______________________________________________________________________________

Don Grimes, Microscopy Today





From: gwe-at-biotech.ufl.edu (Greg Erdos)
Date: Fri, 27 Oct 1995 08:46:44 GMT
Subject: Re: Keeping absolute ethanol dry (for biological EM)

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} Due to space restrictions (yes, even in a practically empty country
} like New Zealand!) we have had to dismantle the still with which I
} used to produce our own super-dry ethanol.
}
} } From now on we will be using commercial absolute ethanol for EM
} specimen dehydrations, and I am seeking advice on what to put in the
} opened containers of ethanol to absorb any traces of water. I never
} cared much for molecular sieve pellets because of the dust that comes
} off them.
}
} Any suggestions on what to use, the quantity required and its
} "lifespan" in use, and how to package the desiccant within the
} bottles, will be gratefully received and a summary posted to the list.
}
}
} Regards
}
} Stephen Edgar
}
} Electron Microscope Unit, Pathology Department
} School of Medicine
} University of Auckland
} Private Bag 92019
} Auckland
} New Zealand
}
} email address: s.edgar-at-auckland.ac.nz
} Phone : +64-9-3737599 extn 6473 (GMT + 12h)
} Fax : +64-9-3737459
}
} } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } .} } }

We place the molecular sieve inside a bag of dialysis tubing in order to
avoid the dust problem and add a bit of indicator silica gel to determine
when the useful life of the desiccant is over. This doesn't work for
acetone since the indicator dye is soluble .
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Greg Erdos Phone: 904-392-1295
Scientific Director,
ICBR Electron Microscopy Core Lab
218 Carr Hall Fax 904-846-0251
University of Florida E-mail: gwe-at-biotech.ufl.edu
Gainesville, FL 32611
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-





From: image-at-imsworld.com (Miller)
Date: Fri, 27 Oct 1995 14:07:32 -0400
Subject: Int'l Immunology Workshop - Italy

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Dear Sir,
Please post this notice to your calendar. Thank you.

Ron Miller
e-mail: image-at-imsworld.com



Neuroimmune Summer Workshop for Students and Faculty

June 17-30, 1996 Rimini Italy

Lecture Topics (in part):
Opiate Neurobiology and Immunology
Stress
Significance of microglia in psychiatry
Psychoneuroimmunology

Laboratory Experience (in part; Hands-On)
HPLC and RIA of neuropeptides
Nitric Oxide-amperometric probe
Image Analysis of Immunocytes
In situ hybridization
Cytokine determinations
Confocal Laser Microscopy

Sponsored by the University of Modena, Image Analytics Corp., Nikon, Compix,
World Precision Instruments and BAS

Scholarships are available - enrollment is limited

For application contact:
Dr. Enzo Ottaviani, Dept Animal Biology
Univ Modena, via Barengario 14,
41100 Modena Italy

Fax: (39) 59-581020 (Italy)
Tel: (39) 59-243566
E-mail: enzott-at-220.unimo.it

Please post this notice on your scientific calendar of upcoming seminars or
send E-mail to: image-at-imsworld.com





From: MicroToday-at-aol.com
Date: Fri, 27 Oct 1995 15:52:22 -0400
Subject: More on Lexmark Optra R

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In response to a number of questions received on the Lexmark Optra R:
1) Printing speed is 16 ppm at 600 dpi and 8 ppm at 1200 dpi and uses a
32-bit RISC processor.
2) To print a full 8.5" x 11" page of text, takes about 20 seconds from
"go".
3) Toner comes in two packages - one for approx. 7000 pages is $179.95 and
the other, for approx. 14000 pages is $265.95. These prices are out of the
Misco catelog and one may be able to do better.
4) I previously mentioned the option of providing additional Printer Memory
(I called it "RAM"). One can also purchase a Flash Memory Option to store
information like downloaded fonts and macros.
5) The printer has two connections on the system board that can be used for
either a Disk Option or a Network Option (or two Network Options, or one of
each).
Disk Option: A 40MB hard disk and a thumbscrew - for example, for
downloaded fonts and macros.
Network Option: To connect directly to a LAN - Token-Ring, Ethernet
(10BASE2 or 10 BASE-T) or LocalTalk (whatever that is?). This option
consists of a network card, a thumbscrew, a diskette containing the Network
Printer Utility and documentation. You will need to provide the appropriate
network cable.
6) I do not know the prices of any of the options but you can call
(800)358-5835 for the name of a dealer near you.
7) In case of a problem, they will express an exchange printer OR repair and
return yours. Apparently there is an on-site warranty repair service which I
do not have. IBM, however, can provide local service.
Lastly - in my previous note I may not have advised how REALLY easy this
printer is to operate. I have had mine for a month now - and have not
refered to the manual even once! Even in start-up, the printer "looked at"
my computer software and adjusted itself to my previous printer driver.
Prior to purchasing this unit, I looked at many high end laser printers and
could not find any, at any price, that would even start to compare.
I welcome any other inquiries,
Don Grimes, Microscopy Today





From: NJWS-at-aol.com
Date: Fri, 27 Oct 1995 17:05:27 -0400
Subject: Re: KODAK 5302 35 mm film

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At the request of Dr Garber,I am only too happy to identify myself to the
list.
My name is Norm Woodside and I am a manufacturer's representative for Ted
Pella,Inc in the middle Atlantic area.
If anyone was offended by this omission in my earlier message,for this I
apologize. Otherwise, I stand by my comments.
I will be happy to review any comments from the list members, but preferrably
in private so as not to take up any netspace, unless of course you feel it of
value to the entire list
Thanks for your attention

Norm Woodside
NJW Supplies
410 744-9574




From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Sat, 28 Oct 1995 15:07:05 GMT+1200
Subject: Re: SEM: Lion S vacuum pump oil

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} Date sent: Thu, 26 Oct 1995 15:47:45 -0600
} From: bozzola-at-siu.edu (John. J. Bozzola)
} Subject: SEM: Lion S vacuum pump oil
} To: microscopy-at-aaem.amc.anl.gov

} A friend has a Hitachi S570 SEM that uses Lion-S (Japanese company)
} diffusion pump oil and needs to replace some of the oil in his 500W dp's.
} Anyone know of a vendor in the US? Thank you very much.
}
} #############################################################################
} John J. Bozzola, Director
} Center for Electron Microscopy
} Southern Illinois University
} Carbondale, IL 62901-4402
} U.S.A.
} Phone: 618-453-3730
} Fax: 618-453-2665
} Email: bozzola-at-siu.edu
} Web: http://www.siu.edu/departments/shops/cem.html
} #############################################################################
}
In Australasia Lion oil available through JEOL. It's the oil
specified for several JEOL diff pumps, it's cheap and, in my old JEOL
probe gives a better vacuum than does Fomblin.

Ritchie Sims phone: 61 9 3737599 ext 7713
Department of Geology fax: 61 9 3737435
University of Auckland
Private Bag 92019
Auckland
New Zealand




From: Francisco J. Hernandez :      fjhblasq-at-biomed.icb2.usp.br
Date: Sat, 28 Oct 1995 00:27:17 -0300 (EST)
Subject: EM: grids and pizza

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I'm curious about a fact that I have noticed when
I'm studying EM sections. Some colleagues have also
observed this: the image observed in the EM fluorescent
ecran appears to be poor contrasted when I begin for the first time, but
after some minutes (15 or 20) the contrast of the material
improves! When the grid is observed for the second time, another
day, the contrast is even better!
Why?
The EM sections are like pizza, that is, they are better the
day after?
I'm just curious.
Francisco Javier Hernandez Blazquez





From: Francisco J. Hernandez :      fjhblasq-at-biomed.icb2.usp.br
Date: Sat, 28 Oct 1995 00:27:17 -0300 (EST)
Subject: EM: grids and pizza

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I'm curious about a fact that I have noticed when
I'm studying EM sections. Some colleagues have also
observed this: the image observed in the EM fluorescent
ecran appears to be poor contrasted when I begin for the first time, but
after some minutes (15 or 20) the contrast of the material
improves! When the grid is observed for the second time, another
day, the contrast is even better!
Why?
The EM sections are like pizza, that is, they are better the
day after?
I'm just curious.
Francisco Javier Hernandez Blazquez





From: bozzola-at-siu.edu (John. J. Bozzola)
Date: Sat, 28 Oct 1995 14:20:51 -0600
Subject: Re: EM: grids and pizza

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X-Sender: bozzola-at-saluki-mail.fiber2.siu.edu
Message-Id: {v01510109acb83e79e43b-at-[131.230.99.53]}
Mime-Version: 1.0
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QUESTION:
} I'm curious about a fact that I have noticed when
} I'm studying EM sections. Some colleagues have also
} observed this: the image observed in the EM fluorescent
} ecran appears to be poor contrasted when I begin for the first time, but
} after some minutes (15 or 20) the contrast of the material
} improves! When the grid is observed for the second time, another
} day, the contrast is even better!
} Why?
} The EM sections are like pizza, that is, they are better the
} day after?
} I'm just curious.
} Francisco Javier Hernandez Blazquez

RESPONSE:
Due to the heating of the section by the beam as well as the vacuum
conditions inside the microscope column, the plastic embedment is
undergoing sublimation - similar to what happens during freeze etching.
With removal of the plastic, the remaining osmicated portions of the
section will increase in contrast. In addition, the plastic is stabilized
in the beam so that drifting is minimized. This phenomenon is so beneficial
that some of the newer microscopes have a special feature that
automatically traverses the beam over the section to stabilize the section
and etch away plastic. A much more perplexing phenomenon, however, is why
pizza does taste better the next day. Perhaps some in-depth research is in
order here.
PEACE.



#############################################################################
John J. Bozzola, Director
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
#############################################################################






From: Chris Jefferies :      chris-at-stowey.demon.co.uk
Date: Sat, 28 Oct 1995 21:18:41 GMT
Subject: Microscopy Manual Online

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It seems to me that a manual of microscopy techniques would be
extremely useful, better in several ways than a book. I've set out
my thoughts on this in the following URLs (they contain identical
information but access speed will be better from your closest site).

Europe - {URL:http://www.lars.bbsrc.ac.uk/micro/in-focus/9511.html}
America - {URL:http://metro.turnpike.net/jefferie/in-focus/9511.html}

If the idea interests you, please take a look and let me have some
comments. If there's enough response perhaps we could get a group of
people discussing it more formally by e-mail to hammer out some
consensus views.

Thanks,

Chris
--
-----------------------------------------------------------------------------
| Chris Jefferies E-Mail (home) - chris-at-stowey.demon.co.uk |
| (work) - chris.jefferies-at-bbsrc.ac.uk |
| Microscopy page (UK) {URL: http://www.lars.bbsrc.ac.uk/micro/} |
| (USA) {URL: http://metro.turnpike.net/jefferie/} |
-----------------------------------------------------------------------------




From: IRevenko-at-eworld.com
Date: Sun, 29 Oct 1995 04:35:22 -0800
Subject: unscribe

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Hi !
Could someone tell me how to disconnect from this list server ?
Sorry for this and thank you.
Irene




From: LSFY69A-at-PRODIGY.COM (MR KARL H BERGER)
Date: Sun, 29 Oct 1995 12:10:53 EST
Subject: Cryo attachment to LKB Nova

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I am looking for a cryo attachment for a LKB Nova Ultramicrotome.
Good money paid if in good
condition. Send reply to LSFY69A-at-prodigy.com





From: zaluzec-at-Microscopy.Com (Nestor J. Zaluzec)
Date: Sun, 29 Oct 1995 20:33:18 -0600
Subject: Part-time Job Opening - IBM East Fishkill, NY

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}
} IBM East Fishkill, NY has an immediate opening for the services of a
} part time TEM professional. 10 to 30 hours/week for several months
} on a renewable basis. Experience with TEM of semiconductors is
} desirable. Interested individuals in the SE New York area may
} contact Ron Anderson (ron-anderson-at-vnet.ibm.com) or
} Dave Kruger (dkruger-at-vnet.ibm.com) for more information.
}
}






From: zaluzec-at-Microscopy.Com (Nestor J. Zaluzec)
Date: Sun, 29 Oct 1995 21:37:38 -0600
Subject: Reply: Advertising Rules on the ListServer

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G'day Subscribers...

I have been asked to repost the section of the ground rules about
advertising on the Microscopy Listserver. Here they are as
extracted from the latest version of the FAQ file. Basically
the rule is no advertising, but Email addresses etc... after
your Sign-Off are acceptable. When I see anyone crossing the
line so-to-speak you can all be assured that the individual will
hear from me.


Your Friendly Neighborhood SysOp
Nestor

-------------------------------

Here is the exerpt from the FAQ file.

--------------------------------


} Can I post an Advertisement?
} ----------------------------
}
} No, that does not fit within the bounds of this discussion forum.
}
} This listserver is not intended to be a Sales mechanism
} for commerical organizations, but rather it is an open discussion area
} about microscopy and microanalysis problems and solutions. If you are
} an
} organization and have equipment you wish to donate,
} or sell, for nominal cost (i.e. no profit) then this is generally
} an acceptable posting. If you are not sure then send a
} copy of the announcement in question to Zaluzec-at-MSA.Microscopy.Com
} and I will give you my opinion. An example of this type
} would be an old decommissioned instrument which someone
} is trying to give away for removal/shipping costs, that would
} fit within the bounds of the purposes of this list.
}
} If you are a manufacturer, you are always welcome to observe/join
} in any discussion at any times. We do ask that everyone, please
} refrain
} from overt sales pitches and/or commericalism. If a product
} which you produce/sell can solve a problem or answer a question
} raised by anyone on this list, then by all means feel free to
} say so. Try to be brief about the product, state the simple facts
} in a few (short) sentences and then offer to continue the discussion
} with
} any interested parties off-line. Alternatively you can give
} sufficient information
} so that individuals can download/access the relevant information.
} Usually
} it will be sufficient to just add your phone number
} and/or Email address to the end of your message,
} and you'll be contacted by anyone that is interested.
}
} Remember, please keep your comments about any product
} you "sell" to a minimum.
}
} It is not out of line to provide your company name, Email
} address or WWW site as part of your signoff/signature line,
} at the end of ANY message you post to this system.
}
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From: minter-at-kodak.com (John Minter)
Date: Mon, 30 Oct 1995 09:30:03 -0400
Subject: Contrast improvement with irradiation

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John J. Bozzola wrote in response to Francisco Javier Hernandez Blazquez:

} Due to the heating of the section by the beam as well as the vacuum
} conditions inside the microscope column, the plastic embedment is
} undergoing sublimation - similar to what happens during freeze etching.
} With removal of the plastic, the remaining osmicated portions of the
} section will increase in contrast. In addition, the plastic is stabilized
} in the beam so that drifting is minimized. This phenomenon is so beneficial
} that some of the newer microscopes have a special feature that
} automatically traverses the beam over the section to stabilize the section
} and etch away plastic.

Electron irradiation damage (radiolysis) is more important than beam
heating. The electron beam cleaves bonds in organic materials, often
producing volatile products that are removed by the vacuum.
Some polymers (e.g. polystyrene, PS) do not lose much mass but are crosslinked
when the radiolysis products react with the polymer. Other polymers
(e.g. polymethylmethacrylate, PMMA) can actually "unzip" to monomer
after an initial cleavage of the chain by the beam. PMMA loses most of
it's mass during heavy irradiation. Most embedding resins are intermediate
between the extremes of PS and PMMA. The classic reference explaining
the chemistry and physics of irradiation damage of polymers is
D. T. Grubb, "Radiation damage and electron microscopy of organic polymers,"
J. Mat. Sci., 9, 1715-1736 (1974).






From: baskin-at-biosci.mbp.missouri.edu (Tobias Baskin)
Date: Mon, 30 Oct 1995 10:12:47 -0600
Subject: Re: EM: grids and pizza

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Message-Id: {v01520d08acbaa76ceeb4-at-[128.206.15.200]}
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Greetings,
At the risk of straying slightly off topic, a question was raised
in this thread:

} RESPONSE:
} Due to the heating of the section by the beam as well as the vacuum
} conditions inside the microscope column, the plastic embedment is
} undergoing sublimation - similar to what happens during freeze etching.
} With removal of the plastic, the remaining osmicated portions of the
} section will increase in contrast. In addition, the plastic is stabilized
} in the beam so that drifting is minimized...
} A much more perplexing phenomenon, however, is why
} pizza does taste better the next day. Perhaps some in-depth research is in

I believe the, shall we say, changes in taste in leftover pizza
arise from slow extraction of flavor molecules from inside cells to the oil
or water surroundings. As spices are commonly bits of plant tissue, the
cell walls are a barrier to extraction, and evidently cooking does not
always drive the process to saturation. Real info about this can probably
be found in one of Hal McGee's books (eg, On Food and Cooking).

Sorry to stray from the topic; please don't flame me, its almost
lunch time.
Tobias Baskin

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
___ ____ ^ ____ _____ Tobias I. Baskin
/ \ / / \ / \ / University of Missouri
/ | / / \ / / Biological Sciences
/___ / /__ /_____\ / /__ 109 Tucker Hall
/ / / \ ( / Columbia, MO 65211 USA
/ / / \ \ / voice: 314-882-0173
/ /____ / \ \____/ /_____ fax: 314-882-0123






From: Ann-Fook Yang :      YANGA-at-em.agr.ca
Date: Mon, 30 Oct 1995 09:28:00 -0500
Subject: Microwave fixation

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Message-Id: {s094c724.080-at-EM.AGR.CA}
X-Mailer: Novell GroupWise 4.1

Fellow microscopists,
I am unable to answer my friend's questions on microwave fixing
and embedding of biological specimens, therefore, I am turning to
you for help.
Is microwave fixing and embedding applicable to all biological
specimens? Has this technique been adopted for processing
biological specimens routinely by those who had used it before?
What are the pros and cons of this technique?
If this topic has come up earlier, can someone send me a copy of
the summary? Thank you.

Ann Fook Yang
Agriculture Canada
C.E.F.
Ottawa, On K1A 0C6
Canada





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-aaem.amc.anl.gov
Date: Mon, 30 Oct 1995 13:46:18 -0600 (CST)
Subject: Microscopy Is NOT a Political Forum

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Colleague

While I am sensitive to funding problems all over the world,
the Microscopy Listserver is NOT a soap box for these issues.

DO NOT use it in this manner. I will not hesitate to unsubscribe
users who use it for other than it's original purposes.

Your Friendly Neighborhood SysOp
Nestor




From: William Tivol :      tivol-at-wadsworth.org
Date: Mon, 30 Oct 1995 14:31:38 -0500 (EST)
Subject: Re: EM & x-ray xtallog

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}
} Hi Everyone,
}
} Does anyone have any ideas on how to combine data
} from Electron microscopy reconstruction and X-ray
} crystallography in image analysis when the complex
} structure obtained from EM and some components'
} structure came from CRYSTALLOGRAPHY?
}
Dear Jinghua,
You could try to calculate the contribution to your image from the
x-ray info using a simulation program (NCEMSS is available from Mike O'Keefe).
If you subtract that contribution, perhaps image reconstruction will be
better, but, since the x-ray data are better than the reconstruction, there
might not be much effect. That is, the reconstruction of anything which is
not crystalline will be about the same whether or not you subtract the known,
crystalline, part. If the complex structure itself is crystalline, a better
idea is to try electron crystallography using molecular replacement and other
direct methods (including getting phases from the image) to phase the data;
then you can reconstruct the specimen from the diffraction data. Good luck.
Yours,
Bill Tivol




From: Fermin, Cesar :      fermin-at-TMC.Tulane.Edu
Date: 30 Oct 1995 16:20:47 -0500
Subject: Wanted Used TEM

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Message-Id: {n1397055278.45049-at-msmail.tmc.tulane.edu}

As you remmebe,r immediately after I donated our old Philips 300 TEM last year
I identified the need for similar instrument by the Instituto Oncologico
Regional del Cibao in the Dominican Republic. The medical director of this
non-profit medical facility still needs on an old TEM (by the way the TEM
will be operated by S Bencosme, whom you may recalled pulished several papers
in the early 60's about the nucleolar organizer region). The institute
recently received a small donation and is able to offer a small amount of
money for an old instrument in working condition. Any news, respond directly
to me please; or inquire if you need information on the institute's non-profit
service status.

******************************************************************
*Cesar D. Fermin, Ph.D \|T|/ Fax (504) 587-7389 *
*Tulane Medical School /|M|\ Voice Mail (504) 584-2618 *
*Pathology/SL79 \|C|/ Secretary (504) 584-2436 *
*New Orleans La 70112-2699 /|*|\ Lab/Techn. (504) 584-2521 *
* {Fermin-at-tmc.tulane.edu} Dir. Morphological Services *
******************************************************************




From: Gary Login :      glogin-at-bih.harvard.edu
Date: Mon, 30 Oct 1995 16:46:44 -0500
Subject: Re: Microwave fixation

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1) Rapid (i.e.,in seconds) microwave fixation is used with success to fix plant
and animal tissues. In addition, several reports show the benefits of using
microwave fixation to preserve soft tissues encased by hard shells (e.g., clams,
teeth, insects).

Recommended reading:
Login, G. R., & Dvorak, A. M. (1994). Application of microwave fixation
techniques in pathology to neuroscience studies: A review. J Neurosci
Methods, 55, 173-182.
Login, G. R., & Dvorak, A. M. (1994). Methods of microwave fixation for
microscopy. A review of research and clinical applications: 1970-1992. Prog
Histochem Cytochem, 27/4, 1-127.
Kok, L. P., & Boon, M. E. (1992). Microwave Cookbook for Microscopists. (3rd
ed.). Leyden: Coulomb Press.


2) Microwave curing of resins (acrylics and epon substitutes) is also
successful.
Recommended reading:
Giammara, B. (1993). Microwave embedment for light and electron microscopy
using Epoxy resins, LR White, and other polymers. Scanning, 15, 82-87.
Login, G. R., & Dvorak, A. M. (1994). The Microwave Toolbook. A Practical
Guide for Microscopists. Boston: Beth Israel Hospital.


3) Microwave processing is used by some large labs for rapid throughput of
specimens. To date, no automatic microwave processors exist so- although
microwave processing is rapid it is also a bit labor intensive.
Recommended reading:
Leong, A. S.-Y. (1991). Microwave fixation and rapid processing in a large
throughput histopathology laboratory. Pathol, 23, 271-273.
Leong, A. S.-Y. (1993). Microwave techniques for diagnostic laboratories.
Scanning, 15, 88-98.


4) The major problems reported with microwave methods are lack of uniformity and
reproduciblity. ALL microwave ovens have high and low energy areas. The
literature reports several methods for calibrating microwave ovens to improve
results.

Login, G. R., & Dvorak, A. M. (1994). The Microwave Toolbook. A Practical
Guide for Microscopists. Boston: Beth Israel Hospital.
Giberson, R. T., & Demaree, R. S., Jr (1995). Microwave fixation: understanding
the variables to achieve rapid reproducible results. Microsc Res Tech, 32,
246-254.
Gu, J., Forte, M., Hance, H., Carson, N., Xenachis, C., & Rufner, R. (1994).
Microwave fixation, antigen retrieval and accelerated immunocytochemistry.
Cell Vision, 1(1), 76-77.

5) Fortunately, many companies which sell Microscopy products have also
designed, tested, and now sell microwave devices and accessories which improve
microwave methods and most importantly make them safer to use in the laboratory.


Please contact me if you have additional questions.

Gary R. Login, D.M.D., D.M.Sc.
Assistant Professor of Oral Pathology
Beth Israel Hospital
Department of Pathology
330 Brookline Avenue
Boston, Massachusetts, 02215

glogin-at- bih.harvard.edu
Telephone: 617-667-2034
Fax: 617-667-8676





From: Murphy, Judy :      murphy-at-ms.sjdccd.cc.ca.us
Date: 30 Oct 1995 16:46:25 -0800
Subject: Need Varian,Mikros Bell Jar

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Message-ID: {n1397050779.73107-at-ms.sjdccd.cc.ca.us}

We are in need of a bell jar for our Varian, Mikros Vacuum Evaporator and
don't have $300 for a new one.
Diameter 10 1/4 inch or 26 cm
Height 1 1/2 in or 29 cm (but could be 30.5 cm).

Anyone having one that doesn't need it, please let me know.
Respond directly to my e mail address.
Thanks
Judy Murphy

Judy Murphy, PhD
Dept. of Microscopy
San Joaquin Delta College
5151 Pacific Ave
Stockton, CA 95207

Phone: 209/474-5284
FAX: 209/474-5649
e-mail: murphy.ms.sjdccd.cc.ca.us





From: ENERGY BEAM SCIENCES, INC :      75767.640-at-CompuServe.COM (by way of W.Jablonski-at-csl.utas.edu.au (Wis Jablonski))
Date: Tue, 31 Oct 1995 14:15:42 +1000
Subject: Re: EPMA:Probe standard for Co

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Message-Id: {199510310315.OAA17942-at-lab.csl.utas.edu.au}
X-Sender: wis-at-lab.csl.utas.edu.au
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

For those of you interested in experimenting, I have found a source of foils
0.025mm thick of the following cobalt compounds:
C069/B12/Si12/Fe4/Mo2/Nil
Co66/Si16/B12/Fe4/Mo2
Co66/Si15/B14/Fe4/Nil
Steven Slap, Vice-President
Energy Beam Sciences, Inc.

Dear all, you can try ASTIMEX Scientific Ltd, mount MINM25-53 with cobaltite
CoAsS ( S- 20.21%, Fe-7.61, Co-21.63, Ni-7.07 and As 43.47 ).
Cheers, Wis Jablonski at W.Jablonski-at-csl.utas.edu.au





From: Jinghua Tang :      jinghua-at-pro.sb.fsu.edu
Date: Mon, 30 Oct 1995 23:33:20 -0500
Subject: EM & x-ray xtallog

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"Re: EM & x-ray xtallog" (Oct 30, 2:31pm)
References: {199510301931.OAA23469-at-wadsworth.ph.albany.edu}
X-Mailer: Z-Mail (3.2.0 26oct94 MediaMail)
To: William Tivol {tivol-at-wadsworth.org}

Dear Bill,
Thank you for your information!
Thinking about the simulation of EM data from X-ray data,
is there any difference between the density map calculated
seperatedly from EM and X-ray data? Is there any function
could be used to calculate the atomic scattering factors
for elcetrons?

Thank you and all who will talk about this topic!

Jinghua Tang

--




From: sinkler :      sinkler-at-dvibm3.gkss.de
Date: Tue, 31 Oct 1995 09:23:10 +0000 (MET)
Subject: Structure info from XRD

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Dear List Subscribers:

Although this question does not really concern microscopy, I believe
it will be of interest to physics and materials science subscribers.

The question was prompted by the correspondence between Jinghua Tang
and Bill Tivol. This involves combining structure data from x-ray
diffraction (such as radial density information) with TEM structural
data from electron microscopy reconstruction.

My problem may be similar in that it involves an amorphous material
(based on XRD) which shows some fringe-like contrast in areas about
2-3 nm in diameter. One way to compare this with similar amorphous
materials would be to obtain the structure factor S(Q) from XRD. I
have found a nice discussion of determination of S(Q) from conventional
XRD in the book by Klug and Alexander. However, I am a bit daunted
at the complexity. I am wondering whether any software (preferably
shareware) exists which calculates S(Q), prompting the user for the
various inputs (sample density, composition, geometry, incident beam,
etc.).

I would be grateful for any information leading to such software, or
any other comments on this endeavor.

Wharton Sinkler
sinkler-at-dvibm3.gkss.de

GKSS Forschungszentrum
Abteilung WA
D-21502 Geesthacht
Germany




From: ENERGY BEAM SCIENCES, INC :      75767.640-at-CompuServe.COM
Date: 31 Oct 95 08:10:40 EST
Subject: Re: Microwave fixation

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Gary Login {glogin-at-bih.harvard.edu}

A good overview of microwave fixation techniques can be found in the Microwave
Cookbook for Microscopists by Drs. Kok & Boon, Coulomb Press, Lyden, 1992, which
is available in Canada from Marivac. More recent articles by Gary Login are
also well worth looking at- please contact me directly for references. General
information can also be found at our home page-
http://www.mwrn.com/ebs/ebs.htm
Steven E. Slap, Vice-President
Energy Beam Sciences, Inc.





From: ENERGY BEAM SCIENCES, INC :      75767.640-at-CompuServe.COM
Date: 31 Oct 95 08:10:47 EST
Subject: Re: EPMA:Probe standard for Co

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"Dear all, you can try ASTIMEX Scientific Ltd, mount MINM25-53 with cobaltite
CoAsS ( S- 20.21%, Fe-7.61, Co-21.63, Ni-7.07 and As 43.47).
Cheers, Wis Jablonski at W.Jablonski-at-csl.utas.edu.au"

This is indeed true, as I believe was already pointed out by Dr. Charles Garber.
The Astimex standard is available both from SPI Supplies and Energy Beam
Sciences. My point was just that the Astimex standard contains 53 minerals and
costs more than $2000, so, if someone simply wants to experiment with various
cobalt compounds, they might prefer other options.
Steven Slap, Vice-President
Energy Beam Sciences, Inc.





From: ldm2-at-apollo.numis.nwu.edu (L.D.Marks)
Date: Tue, 31 Oct 1995 07:44:26 -0600
Subject: Re: Structure info from XRD

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I think that this is a case where sophisticated analyses are not
required. Provided that the microscope is not misaligned so we
can ignore astigmatism and beam tilt, it is a standard result that
HREM is more sensitive to local crystallinity than x-ray diffraction.
What you describe is fairly common; the x-ray powder pattern appears
to show little to no structure due to Debye-Scherrer broadening with
nanoscale crystalline regions.

In other words, this is a partialy crystalline or nanocrystalline
material, not an amorphous one.




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-aaem.amc.anl.gov
Date: Tue, 31 Oct 1995 11:36:22 -0600 (CST)
Subject: Political Comments That Some Didn't See

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G'day Colleagues,

My posting about no political messages was in response to the
message entitled...

RE: CANADIAN PROFESSORS STRIKE FOR ACADEMIC FREEDOM

Not all of you got copies of it as I managed
to catch it (by dumb luck) before it went to the entire list.
I just happend to be logged in when the initial message came
through and stopped it before it was distributed to the whole
list.

Clearly messages like this have no place on the Microscopy List.

Your Friendly Neighborhood SysOp

Nestor





From: ldm2-at-apollo.numis.nwu.edu (L.D.Marks)
Date: Tue, 31 Oct 1995 07:51:50 -0600
Subject: Re: EM & x-ray xtallog

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With respect to a comparison of the density map from x-ray
and electron diffraction data, remember that this comparison
is only valid in the limit where electron diffraction can be
considered as kinematical. With this taken into account,
x-ray scattering factors (via the Mott formula for conversion)
and electron scattering factors give identical results, except
perhaps at very low angles and/or at the very precise level of
fractions of a percent (e.g. see some of John Spence's work).
Many multislice programs (and probably also Bloch-Wave programs)
use either type of scattering factors with ease.

At least at the visual level, we once compared a (kinematical)
electron diffraction Patterson function with someone else's
x-ray Patterson function and they appeared to be the same.




From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 31 Oct 1995 13:43:23 -0400
Subject: RE-Lion-S DP Oil

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Message-ID: {n1396975330.59938-at-mse.engin.umich.edu}

Subject: Time: 1:16 PM
OFFICE MEMO RE:Lion-S DP Oil Date: 10/31/95

We faced the problem of replacing Lion-S oil in the diffusion pump of our
Hitachi S520 SEM several years ago. At that time, I investigated the matter,
and was informed that Lion-S oil is di-(2-ethylhexyl) sebacate, a diester oil
that was developed by CVC Products, Inc (525 Lee Road, P.O. Box 1886,
Rochester, NY; Ph: 800-448-5900; Fx: 716-458-0424) as one of the first
synthetic diffusion pump oils to come into use (see Sect. 5.4.2 of my book,
"Vacuum Methods in Electron Microscopy"), and marketed by them under the
trade name OCTOIL-S (CVC Stock No: 60786-0031). The same material can also
be purchased from Kurt J. Lesker, Inc. (1515 Worthington Ave, Clairton, PA
15025; Ph: 800-245-1656; Fx:41`2-233-4275) under the trade name DIFFOIL-S
(Stock No. DIFFOILSBB). The price is about $70 for 500 CC. There are
undoubtedly numerous other sources, but these are the first two I came up
with - no prejudice intended, just trying to be helpful.
W. C. Bigelow (bigelow-at-umich.edu)





From: William Tivol :      tivol-at-wadsworth.org
Date: Tue, 31 Oct 1995 13:39:21 -0500 (EST)
Subject: Re: Structure info from XRD

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Alternate-Recipient: allowed
Auto-Forwarded: prohibited
Content-Return: allowed
Disclose-Recipients: prohibited
Conversion: allowed
Importance: normal
Priority: normal
Sensitivity: Company-Confidential
Microscopy ListServer {Microscopy-at-Sparc5.Microscopy.Com}
Message-Id: {951031081338.726-at-cliff.ml.wpafb.af.mil.0}

Dear Wharton,
This sounds like the same phenomenon as seen using holey carbon films
to check the astigmatism and resolution. If so, the fringe depends on the
focus value and lens parameters, and trying to derive structural information
from the fringe would seem to be extrordinarily complex, if possible at all.
Yours,
Bill Tivol




From: jfmjfm-at-umich.edu (John F. Mansfield)
Date: Tue, 31 Oct 1995 21:31:42 -0400
Subject: Re: Microwave fixation

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X-Sender: jfmjfm-at-srvr5.engin.umich.edu
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Although not from a company this looks much like a shameless commercial to
seel this guy's books, what do you think?

} 1) Rapid (i.e.,in seconds) microwave fixation is used with success to fix
} plant
} and animal tissues. In addition, several reports show the benefits of using
} microwave fixation to preserve soft tissues encased by hard shells (e.g.,
} clams,
} teeth, insects).
}
} Recommended reading:
} Login, G. R., & Dvorak, A. M. (1994). Application of microwave fixation
} techniques in pathology to neuroscience studies: A review. J Neurosci
} Methods, 55, 173-182.
} Login, G. R., & Dvorak, A. M. (1994). Methods of microwave fixation for
} microscopy. A review of research and clinical applications: 1970-1992. Prog
} Histochem Cytochem, 27/4, 1-127.
} Kok, L. P., & Boon, M. E. (1992). Microwave Cookbook for Microscopists. (3rd
} ed.). Leyden: Coulomb Press.
}
}
} 2) Microwave curing of resins (acrylics and epon substitutes) is also
} successful.
} Recommended reading:
} Giammara, B. (1993). Microwave embedment for light and electron microscopy
} using Epoxy resins, LR White, and other polymers. Scanning, 15, 82-87.
} Login, G. R., & Dvorak, A. M. (1994). The Microwave Toolbook. A Practical
} Guide for Microscopists. Boston: Beth Israel Hospital.
}
}
} 3) Microwave processing is used by some large labs for rapid throughput of
} specimens. To date, no automatic microwave processors exist so- although
} microwave processing is rapid it is also a bit labor intensive.
} Recommended reading:
} Leong, A. S.-Y. (1991). Microwave fixation and rapid processing in a large
} throughput histopathology laboratory. Pathol, 23, 271-273.
} Leong, A. S.-Y. (1993). Microwave techniques for diagnostic laboratories.
} Scanning, 15, 88-98.
}
}
} 4) The major problems reported with microwave methods are lack of
} uniformity and
} reproduciblity. ALL microwave ovens have high and low energy areas. The
} literature reports several methods for calibrating microwave ovens to improve
} results.
}
} Login, G. R., & Dvorak, A. M. (1994). The Microwave Toolbook. A Practical
} Guide for Microscopists. Boston: Beth Israel Hospital.
} Giberson, R. T., & Demaree, R. S., Jr (1995). Microwave fixation:
} understanding
} the variables to achieve rapid reproducible results. Microsc Res Tech, 32,
} 246-254.
} Gu, J., Forte, M., Hance, H., Carson, N., Xenachis, C., & Rufner, R. (1994).
} Microwave fixation, antigen retrieval and accelerated immunocytochemistry.
} Cell Vision, 1(1), 76-77.
}
} 5) Fortunately, many companies which sell Microscopy products have also
} designed, tested, and now sell microwave devices and accessories which improve
} microwave methods and most importantly make them safer to use in the
} laboratory.
}
}
} Please contact me if you have additional questions.
}
} Gary R. Login, D.M.D., D.M.Sc.
} Assistant Professor of Oral Pathology
} Beth Israel Hospital
} Department of Pathology
} 330 Brookline Avenue
} Boston, Massachusetts, 02215
}
} glogin-at- bih.harvard.edu
} Telephone: 617-667-2034
} Fax: 617-667-8676

John Mansfield
North Campus Electron Microbeam Analysis Laboratory
413 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (313)936-3352 FAX (313)936-3352
jfmjfm-at-engin.umich.edu, John.F.Mansfield-at-umich.edu
or jfmjfm-at-umich.edu they all reach me!
URL: http://wwwpersonal.engin.umich.edu/~jfmjfm/jfmjfm.html






From: richard.easingwood-at-stonebow.otago.ac.nz (Richard Easingwood)
Date: Wed, 1 Nov 1995 09:09:30 +1100
Subject: Thanks for the Freeze slam info/ BioRad Gold agents?

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Thanks very much to those people who replied to my request for infomation
about freeze slamming cultured cells.

I have had a question passed on to me about BioRad immunogold starter kits.
These starter kits provided material for an introduction to EM
immunocytochemistry. They were aimed as an introduction to researchers and
as a convenient and economically priced kit for classroom teaching.

Does anyone know what company ended up with the BioRad Gold part of the
Microscience division when it was split up?

Does anyone know of any other immunocytochemical supplier who supplies
"starter kits"?

Regards,


Richard E


Richard Easingwood
South Campus Electron Microscope Unit
Otago Medical School
PO Box 913
Dunedin
NEW ZEALAND

Telephone: 64-03-479 7301
Facsimile: 64-03-479 7254


The Southernmost EM Unit in the World






From: howard-at-CSHL.org (Tamara Howard in Cold Spring Harbor Laboratory)
Date: Tue, 31 Oct 1995 18:31:49 -0500
Subject: LM:coverslip carriers?

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Does anyone have a source for racks (metal, plastic...) that will hold
glass coverslips for staining? Several of us have used metal racks in the
past that would hold at least 10 coverslips - now we can only find smaller
ones in the catalogues. Preferably for 22x22mm cs, but we'll take anything
anyone sells!
Thanks!
Tamara Howard
Cold Spring Harbor Lab
howard-at-cshl.org






From: bozzola-at-siu.edu (John. J. Bozzola)
Date: Wed, 1 Nov 1995 04:52:44 -0600
Subject: Re: Microwave fixation

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THE QUESTION WAS ASKED EARLIER:
} Although not from a company this looks much like a shameless commercial to
} seel this guy's books, what do you think?

MY REACTION:
Having just reviewed the area of microwave fixation, I can tell you that
there are 4-5 major contributors who have been involved in the development
of this technology from the very beginning. Dr. Login is one of the major
contributors in this area and he has referenced the significant
contributions appropriate for the question posted earlier by someone just
starting out with microwave fixation.




#############################################################################
John J. Bozzola, Director
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
#############################################################################






From: John Getty :      jgetty-at-du.edu
Date: Tue, 31 Oct 1995 15:39:45 -0700 (MST)
Subject: SEM Wanted

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Greetings,

The Engineering Department at the University of Denver would like to
acquire a used field emission SEM with an Energy Dispersive X-Ray Analysis
System. An Hitachi model S800 would fit our needs precisely.

The Engineering Department at DU offers ABET accredited BS and MS degrees
in Mechanical and Electrical Engineering, and a PhD in Material Science.
Our current enrollment includes about 110 undergrads, and 21 grad
students.

Our Material Science laboratory, operated by Dr. Paul Predecki, is
equipped with fairly modern x-ray diffraction equipment. However, our
existing SEM has not responded to efforts at resuscitation, and has become
a fiscal black hole.

As a private non-profit we are limited in the financial resources
available to us. We have, however, set aside some funds to assist with the
SEM acquisition.

Please contact me directly if you have any leads. I can be reached at
jgetty-at-du.edu, or (303)871-3129.

Thanks,

John Getty
Laboratory Director, Engineering
University of Denver
Denver, CO 80208







From: tphillips-at-biosci.mbp.missouri.edu (Tom Phillips)
Date: Tue, 31 Oct 1995 18:18:35 -0600
Subject: Re: Microwave fixation

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I see no reason for Dr. Login not to be able to cite the key references in
the field even tho he co-authored them. I think he should be commended for
taking the time and pointing them out to the individual. I passed the list
on to one of my students who had recently expressed interest in playing
with microwave techniques. You don't have to buy his book - you can simply
go to the library. I also note he cites primary references in the
literature and the main competitor to his book (the microwave cookbook).
Its negative comments like this that turn people off from replying to
simple queries. For the record, I don't have any financial interest in any
of the publishers and I am not a friend of Dr. Login. I did meet him at a
meeting once and we had a pleasant 15 min discussion in which he happily
gave me some hints about a microwave technique I was trying.




} Although not from a company this looks much like a shameless commercial to
} seel this guy's books, what do you think?
}
} } 1) Rapid (i.e.,in seconds) microwave fixation is used with success to fix
} } plant
} } and animal tissues. In addition, several reports show the benefits of using
} } microwave fixation to preserve soft tissues encased by hard shells (e.g.,
} } clams,
} } teeth, insects).
} }
} } Recommended reading:
} } Login, G. R., & Dvorak, A. M. (1994). Application of microwave fixation
} } techniques in pathology to neuroscience studies: A review. J Neurosci
} } Methods, 55, 173-182.
} } Login, G. R., & Dvorak, A. M. (1994). Methods of microwave fixation for
} } microscopy. A review of research and clinical applications: 1970-1992. Prog
} } Histochem Cytochem, 27/4, 1-127.
} } Kok, L. P., & Boon, M. E. (1992). Microwave Cookbook for Microscopists. (3rd
} } ed.). Leyden: Coulomb Press.
} }
} }
} } 2) Microwave curing of resins (acrylics and epon substitutes) is also
} } successful.
} } Recommended reading:
} } Giammara, B. (1993). Microwave embedment for light and electron microscopy
} } using Epoxy resins, LR White, and other polymers. Scanning, 15, 82-87.
} } Login, G. R., & Dvorak, A. M. (1994). The Microwave Toolbook. A Practical
} } Guide for Microscopists. Boston: Beth Israel Hospital.
} }
} }
} } 3) Microwave processing is used by some large labs for rapid throughput of
} } specimens. To date, no automatic microwave processors exist so- although
} } microwave processing is rapid it is also a bit labor intensive.
} } Recommended reading:
} } Leong, A. S.-Y. (1991). Microwave fixation and rapid processing in a large
} } throughput histopathology laboratory. Pathol, 23, 271-273.
} } Leong, A. S.-Y. (1993). Microwave techniques for diagnostic laboratories.
} } Scanning, 15, 88-98.
} }
} }
} } 4) The major problems reported with microwave methods are lack of
} } uniformity and
} } reproduciblity. ALL microwave ovens have high and low energy areas. The
} } literature reports several methods for calibrating microwave ovens to improve
} } results.
} }
} } Login, G. R., & Dvorak, A. M. (1994). The Microwave Toolbook. A Practical
} } Guide for Microscopists. Boston: Beth Israel Hospital.
} } Giberson, R. T., & Demaree, R. S., Jr (1995). Microwave fixation:
} } understanding
} } the variables to achieve rapid reproducible results. Microsc Res Tech, 32,
} } 246-254.
} } Gu, J., Forte, M., Hance, H., Carson, N., Xenachis, C., & Rufner, R. (1994).
} } Microwave fixation, antigen retrieval and accelerated immunocytochemistry.
} } Cell Vision, 1(1), 76-77.
} }
} } 5) Fortunately, many companies which sell Microscopy products have also
} } designed, tested, and now sell microwave devices and accessories which improve
} } microwave methods and most importantly make them safer to use in the
} } laboratory.
} }
} }
} } Please contact me if you have additional questions.
} }
} } Gary R. Login, D.M.D., D.M.Sc.
} } Assistant Professor of Oral Pathology
} } Beth Israel Hospital
} } Department of Pathology
} } 330 Brookline Avenue
} } Boston, Massachusetts, 02215
} }
} } glogin-at- bih.harvard.edu
} } Telephone: 617-667-2034
} } Fax: 617-667-8676
}
} John Mansfield
} North Campus Electron Microbeam Analysis Laboratory
} 413 SRB, University of Michigan
} 2455 Hayward, Ann Arbor MI 48109-2143
} Phone: (313)936-3352 FAX (313)936-3352
} jfmjfm-at-engin.umich.edu, John.F.Mansfield-at-umich.edu
} or jfmjfm-at-umich.edu they all reach me!
} URL: http://wwwpersonal.engin.umich.edu/~jfmjfm/jfmjfm.html

Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility
3 Tucker Hall
University of Missouri
Columbia, MO 65211
(314)-882-4712 (voice)
(314)-882-0123 (fax)






From: Gary Login :      glogin-at-bih.harvard.edu
Date: Mon, 30 Oct 1995 16:46:44 -0500
Subject: Re: Microwave fixation

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Fellow microscopists,

In response to John Mansfield's recent comment about commercialism on the list
server.

My E-mail on Microwave Fixation (Oct 30th) was sent in response Dr. Yang's
queries about microwave fixation posted on the microscopy list server (Oct
30th). I did not repeat Dr. Yang's message in my E-mail. IN the future I will
make reference to or include the message I am replying to to avoid confusion.

John Mansfield's E-mail, Dr. Yangs' E-mail, and my E-mail follow for the sake of
sending a complete message.
------------------------------

Although not from a company this looks much like a shameless commercial to
seel this guy's books, what do you think?


----------------


Fellow microscopists,
I am unable to answer my friend's questions on microwave fixing
and embedding of biological specimens, therefore, I am turning to
you for help.
Is microwave fixing and embedding applicable to all biological
specimens? Has this technique been adopted for processing
biological specimens routinely by those who had used it before?
What are the pros and cons of this technique?
If this topic has come up earlier, can someone send me a copy of
the summary? Thank you.

Ann Fook Yang
Agriculture Canada
C.E.F.
Ottawa, On K1A 0C6
Canada
------------------------


1) Rapid (i.e.,in seconds) microwave fixation is used with success to fix plant
and animal tissues. In addition, several reports show the benefits of using
microwave fixation to preserve soft tissues encased by hard shells (e.g., clams,
teeth, insects).

Recommended reading:
Login, G. R., & Dvorak, A. M. (1994). Application of microwave fixation
techniques in pathology to neuroscience studies: A review. J Neurosci
Methods, 55, 173-182.
Login, G. R., & Dvorak, A. M. (1994). Methods of microwave fixation for
microscopy. A review of research and clinical applications: 1970-1992. Prog
Histochem Cytochem, 27/4, 1-127.
Kok, L. P., & Boon, M. E. (1992). Microwave Cookbook for Microscopists. (3rd
ed.). Leyden: Coulomb Press.


2) Microwave curing of resins (acrylics and epon substitutes) is also
successful.
Recommended reading:
Giammara, B. (1993). Microwave embedment for light and electron microscopy
using Epoxy resins, LR White, and other polymers. Scanning, 15, 82-87.
Login, G. R., & Dvorak, A. M. (1994). The Microwave Toolbook. A Practical
Guide for Microscopists. Boston: Beth Israel Hospital.


3) Microwave processing is used by some large labs for rapid throughput of
specimens. To date, no automatic microwave processors exist so- although
microwave processing is rapid it is also a bit labor intensive.
Recommended reading:
Leong, A. S.-Y. (1991). Microwave fixation and rapid processing in a large
throughput histopathology laboratory. Pathol, 23, 271-273.
Leong, A. S.-Y. (1993). Microwave techniques for diagnostic laboratories.
Scanning, 15, 88-98.


4) The major problems reported with microwave methods are lack of uniformity and
reproduciblity. ALL microwave ovens have high and low energy areas. The
literature reports several methods for calibrating microwave ovens to improve
results.

Login, G. R., & Dvorak, A. M. (1994). The Microwave Toolbook. A Practical
Guide for Microscopists. Boston: Beth Israel Hospital.
Giberson, R. T., & Demaree, R. S., Jr (1995). Microwave fixation: understanding
the variables to achieve rapid reproducible results. Microsc Res Tech, 32,
246-254.
Gu, J., Forte, M., Hance, H., Carson, N., Xenachis, C., & Rufner, R. (1994).
Microwave fixation, antigen retrieval and accelerated immunocytochemistry.
Cell Vision, 1(1), 76-77.

5) Fortunately, many companies which sell Microscopy products have also
designed, tested, and now sell microwave devices and accessories which improve
microwave methods and most importantly make them safer to use in the laboratory.


Please contact me if you have additional questions.

Gary R. Login, D.M.D., D.M.Sc.
Assistant Professor of Oral Pathology
Beth Israel Hospital
Department of Pathology
330 Brookline Avenue
Boston, Massachusetts, 02215

glogin-at- bih.harvard.edu
Telephone: 617-667-2034
Fax: 617-667-8676




Gary R. Login, D.M.D., D.M.Sc.
Assistant Professor of Oral Pathology
Beth Israel Hospital
Department of Pathology
330 Brookline Avenue
Boston, Massachusetts, 02215

glogin-at- bih.harvard.edu
Telephone: 617-667-2034
Fax: 617-667-8676





From: Michael Bosma :      Michael.Bosma-at-vf.slu.se
Date: Wed, 1 Nov 1995 14:17:22 +0100
Subject: subscribtion on listserver

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would like to subscribe to the microscopy listserver

kind regards,

Michael Bosma
******************************************************************************
* *
* Michael Bosma Telephone: +46 418 670 62 *
* The Swedish University of Fax: + 46 418 670 81 *
* Agricultural Sciences E-mail: Michael Bosma-at-vf.slu.se *
* Dept of Plant Breeding Research *
* S-26831 Svaloev, Sweden *
* *
******************************************************************************





From: ENERGY BEAM SCIENCES, INC :      75767.640-at-CompuServe.COM
Date: 01 Nov 95 08:31:55 EST
Subject: Re: BioRad Gold agents?

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For the record, over a period of time the various BioRad consumables ended up
first at Energy Beam Sciences, then at SPI Supplies. Please contact both of
these companies with any questions about products from the old BioRad catalog.
Steven Slap, Vice-President
Energy Beam Sciences, Inc.





From: Paul Webster :      Paul.Webster-at-quickmail.yale.edu
Date: 1 Nov 1995 09:57:41 -0400
Subject: Reduced Osmium

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Message-Id: {n1396902415.72877-at-QuickMail.Yale.edu}

Dear colleagues,
can someone help by providing a good recipe for reducing osmium tetroxide to
help increase contrast in epoxy resin - embedded biological material? Is
potassium ferri-, or ferro- cyanide the best? I thought I knew the answer but a
post-doc here has given me doubts so we have a small wager riding on this.





From: ENERGY BEAM SCIENCES, INC :      75767.640-at-CompuServe.COM
Date: 01 Nov 95 08:31:45 EST
Subject: Re: Microwave fixation

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Message-ID: {edickg.1165621758C-at-mail.its.rpi.edu}
Gary Login {glogin-at-bih.harvard.edu}

John Mansfield responded to Gary Logins' post by asking, "Although not from a
company this looks much like a shameless commercial to
sell this guy's books, what do you think?"
Gary Login is recognized as one of the leading experts in the world on this
subject. I am absolutely convinced that his references to his own articles and
books were made in a sincere effort to be helpful to a fellow microscopist
looking for source material on a technique.
This is far from the first time a microscopist has mentioned his own
publications in a posting to this list (I recall even seeing ISBN numbers).
By the way, I have no commercial interest in Gary's book, which is sold by some
of my esteemed competitors.
Steven Slap, Vice-President
Energy Beam Sciences





From: kennedy-at-nsi.edu (grace kennedy)
Date: Wed, 1 Nov 1995 11:18:06 -0800
Subject: reduced osmium

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I've used potassium ferrOcyanide-but mainly as a membrane enhancer for
neuronal tissues. If I need to just reduce overall darkening by the osmium
I use 7% sucrose in the osmication-seems to slow down that process a bit.
I did find that the ferrocyanide-osmium will destroy delicate tissues not
well fixed in GLA-I measured the pH and as I recall it was quite
high-around 10-11. You also have to be careful of the buffer system you
are in-buffer incompatabilities will give you a fine crystalline
precipitate which cannot be removed. Phosphate is out, cacodylate is OK.
I have not tried this on any HRP-filled material-I have no idea whether or
not it could damage the chromogen... Grace Kennedy..






From: A. Kent Christensen :      akc-at-umich.edu
Date: Wed, 1 Nov 1995 13:45:36 -0500 (EST)
Subject: Re: Reduced Osmium

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While we're on this topic, could I ask a related question? Why does
the usual ferri-ferrocyanide method (Karnovsky 1971, ASCB abstracts; or
Russell and Burguet, 1978, Tissue and Cell 9:751) stain membranes so
well, but leaves ribosomes virtually invisible?

Kent
A. Kent Christensen, University of Michigan, {akc-at-umich.edu}

----------------------------------------

On 1 Nov 1995, Paul Webster wrote:

} Dear colleagues,
} can someone help by providing a good recipe for reducing osmium tetroxide to
} help increase contrast in epoxy resin - embedded biological material? Is
} potassium ferri-, or ferro- cyanide the best? I thought I knew the answer but a
} post-doc here has given me doubts so we have a small wager riding on this.
}
}




From: Ann-Fook Yang :      YANGA-at-em.agr.ca
Date: Wed, 01 Nov 1995 14:55:58 -0500
Subject: Re. Microwave fixation

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Message-Id: {s0978b08.050-at-EM.AGR.CA}
X-Mailer: Novell GroupWise 4.1

Thanks are due to Drs. Steven Slap and Gary Login who have
responded to my posting .
It was unfortunate that someone thought that Dr. Login was try to
sell his book. I do not see that he has that intention. Dr. Login
not only answered my questions but also suggested a number of
reference to read. His effort must be highly commended. I am sure
that the references and the answers will be useful for my friend
and for anyone who wants to investigate more about this technique.






From: Ray Hicks :      rh208-at-cus.cam.ac.uk
Date: Wed, 1 Nov 1995 12:57:41 +0000 (GMT)
Subject: Re: Microwave fixation

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Well John,
I don't think it matters much. Do you think that there is such a huge
bibliography on the subject that he has purposefully refrained from mentioning
other books so that he can cream off the huge profits that a greater market
share would give? I don't, he seems to have answered the original question
fairly.

John F. Mansfield wrote:

} Although not from a company this looks much like a shameless commercial to
} seel this guy's books, what do you think?
}
} } 1) Rapid (i.e.,in seconds) microwave fixation is used with success to fix
} } plant
} } and animal tissues. In addition, several reports show the benefits of using
} } microwave fixation to preserve soft tissues encased by hard shells (e.g.,
} } clams,
} } teeth, insects).
} }
} } Recommended reading:
} } Login, G. R., & Dvorak, A. M. (1994). Application of microwave fixation
} } techniques in pathology to neuroscience studies: A review. J Neurosci
} } Methods, 55, 173-182.
} } Login, G. R., & Dvorak, A. M. (1994). Methods of microwave fixation for
} } microscopy. A review of research and clinical applications: 1970-1992. Prog
} } Histochem Cytochem, 27/4, 1-127.
} } Kok, L. P., & Boon, M. E. (1992). Microwave Cookbook for Microscopists. (3rd
} } ed.). Leyden: Coulomb Press.
} SNIP


Ray.




From: bozzola-at-siu.edu (John. J. Bozzola)
Date: Thu, 2 Nov 1995 03:00:42 -0600
Subject: LM:purchase of stereomicroscope

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A colleague at our institution needs to purchase a stereomicroscope (aka
"dissecting" stereo scope). Needs: magnification up to 60-80x, trinoc with
Polaroid camera capable of generating positive/negatives. Price limitation
is $3,000. Please contact me with information.

#############################################################################
John J. Bozzola, Director
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
#############################################################################






From: Patty Jansma :      plj-at-manduca.neurobio.arizona.edu
Date: Wed, 01 Nov 1995 14:46:22 -0700 (MST)
Subject: Re: MAP

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You might try Collaborative Biomedical Products at (617) 275-0004. They
carry a product called Cell-Tak which I believe is very similiar to MAP.

Patty Jansma Tel:602-621-6671
plj-at-manduca.neurobio.arizona.edu
Arizona Research Labs Division of Neurobiology
University of Arizona


On Thu, 26 Oct 1995, Goodhouse, Joseph wrote:

}
} I am looking for the company that sells MAP, Mussel Adhesion Protein.
} Thank you.
}
} J. Goodhouse
} Molec. Bio..
} Princeton University







From: lmiller-at-ux1.cso.uiuc.edu (Lou Ann Miller)
Date: Wed, 1 Nov 1995 13:44:23 -0600
Subject: Re: Reduced Osmium

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Greetings,

Both work, but the "ic" works stronger ( too strong sometimes , it gives
uneven and "funny" nuclei if over stained.).

I Fix first in OsO4, and at the end of the incubation, I will add 3% KCN (
"ic " variety) for 10-15 minutes. Helps greatly, and the shorter- later
times keep the funny nucleus effect from happening.

Lou Ann


} Dear colleagues,
} can someone help by providing a good recipe for reducing osmium tetroxide to
} help increase contrast in epoxy resin - embedded biological material? Is
} potassium ferri-, or ferro- cyanide the best? I thought I knew the answer
} but a
} post-doc here has given me doubts so we have a small wager riding on this.

***********************
Lou Ann Miller
Microscopic Imaging Laboratory
College of Veterinary Medicine
University of Illinois
2001 S Lincoln Ave
Urbana, Illinois 61801
217-244-1566
lmiller-at-ux1.cso.uiuc.edu

Microscopy Lab:
http://www.cvm.uiuc.edu/announcements/MicSoc/MicImagLab.html

Personal Home Page:
http://www.cvm.uiuc.edu/HomePages/LouAnnMiller/Homepage.html
***********************






From: jfmjfm-at-umich.edu (John F. Mansfield)
Date: Wed, 1 Nov 1995 23:34:46 -0400
Subject: Re: Microwave fixation, actually continued comment

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OK, so Gary Login's post was not commercially based. I'm sorry. I was
going to send the question to Nestor Zaluzec only but I got distracted and
sent it to the list by mistake. However, I think it does bear some further
comment and/or discussion.
This list is getting very large and there are now many cases of messages
that should not be sent to all subscribers without there being some
indication that a large number of them want to see the responses. It
really shouldnt have been posted to the list at all, and my reasoning for
that is below.

Net etiquette (or Netiquette as it is typically known ) says that if there
is a question in a mailing list or newsgroup then the correct response is
to send a message to the original poster. Often a poster will say "private
replies please, if there is enough interest I will summarize to the
net/list". This should be implicit in ALL posts. In this manner if the
poster receives many replies, and there is much duplication in the replies,
then any summary can be edited by the original poster before sending the
information out to everyone on the list/net. People wishing copies of the
replies also send requests to the original poster.
If the original poster geets say five to ten "me too" requests for the
information
You may ask why should the original poster have to do this editing and
summarizing work? Well they are getting the free info courtesy of others
so it isnt exactly a large price to pay.

Maybe these instructions should be part of the files that gets sent to new
subscribers.

} Although not from a company this looks much like a shameless commercial to
} sell this guy's books, what do you think?
}
} } 1) Rapid (i.e.,in seconds) microwave fixation is used with success to fix
} } plant
} } and animal tissues. In addition, several reports show the benefits of using
} } microwave fixation to preserve soft tissues encased by hard shells (e.g.,
} } clams,
} } teeth, insects).
} }
} } Recommended reading:
} } Login, G. R., & Dvorak, A. M. (1994). Application of microwave fixation
} } techniques in pathology to neuroscience studies: A review. J Neurosci
} } Methods, 55, 173-182.
} } Login, G. R., & Dvorak, A. M. (1994). Methods of microwave fixation for

John Mansfield
North Campus Electron Microbeam Analysis Laboratory
413 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (313)936-3352 FAX (313)936-3352
jfmjfm-at-engin.umich.edu, John.F.Mansfield-at-umich.edu
or jfmjfm-at-umich.edu they all reach me!
URL: http://wwwpersonal.engin.umich.edu/~jfmjfm/jfmjfm.html






From: chandler-at-lamar.ColoState.EDU (John Chandler)
Date: Wed, 1 Nov 1995 13:19:12 -0700
Subject: Re: Microwave fixation

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} John Mansfield responded to Gary Logins' post by asking, "Although not from a
} company this looks much like a shameless commercial to
} sell this guy's books, what do you think?"
} Gary Login is recognized as one of the leading experts in the world on this
} subject. I am absolutely convinced that his references to his own articles and
} books were made in a sincere effort to be helpful to a fellow microscopist
} looking for source material on a technique.
} This is far from the first time a microscopist has mentioned his own
} publications in a posting to this list (I recall even seeing ISBN numbers).
} By the way, I have no commercial interest in Gary's book, which is sold by some
} of my esteemed competitors.
} Steven Slap, Vice-President
} Energy Beam Sciences

I'm really happy about having experts in MANY fields on this list. I'll
admit that I don't know much about microwave-enhanced processing, much less
the folks doing it, even though I'm on the biological side of EM. I have
heard recently that there have been some very significant advances in the
techniques and technology, and look forward to a lot of discussions about
them. I hope everyone who has useful contributions will chime in.

John
chandler-at-lamar.ColoState.EDU






From: BARBARA.HARTMAN-at-1773.220.SCHERING-PLOUGH.sprint.com
Date: Wed, 1 Nov 1995 12:16:33 -0500
Subject: Uranyl acetate and lead citrate disposal

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Greetings:

I stain most of my EM grids by hand but for large numbers of grids,
I prefer to use the Reichert Ultrostainer. Since the Ultrostainer combines
the waste into one container, I am faced with finding a disposal site that
will take the combined waste. It has been difficult for us in the past to
dispose of our waste, but I have just been informed that now no one will
accept it. The radiation sites won't take it because of the lead and the
hazardous waste sites won't take it because of the uranyl. We put
absolutely nothing but water down the drain so public sewers are not an
option. I will be contacting Leica to see if there is a way of separating
the waste, but in lieu of that, how do the rest of you dispose of this
combined waste ?? Any help will be greatly appreciated.


Barbara Hartman
Schering-Plough Research
Lafayette, NJ 07848

E-Mail: Barbara.Hartman-at-Schering-Plough.sprint.com






From: Paul Webster :      Paul.Webster-at-quickmail.yale.edu
Date: 1 Nov 1995 15:11:42 -0400
Subject: Re: reduced osmium

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Message-Id: {n1396883514.10936-at-QuickMail.Yale.edu}

Thank you all for your replies. However, from the diverse replies, you see my
problem.
Here is the original reply I gave when asked:
To reduce osmium from osmium (viii) to osmium (iv), the other component has be
oxidised! Since iron appears in two forms Fe (iii), ferri and Fe (ii) the Fe
(ii) has to be used. And Fe (ii) is ferro-. Therefore it would be reasonable to
assume that ferrocyanide would be the one to use and, in fact, this is what I
have been using for years with excellent results.

I am mystified why others say that the ferricyanide works just as well, and I
know there are are many published papers using this from respected laboratories.
This just makes it all the more mysterious, or am I missing something very
simple here?

I thank everyone who replied, but special thanks must go to the contributors who
supported me (without knowing it):
George Ruben who said - "Potassium ferro- cyanide can reduce osmium tetroxide
and in so doing becomes ferricyanide!"
Ubirajara P. Rodrigues-Filho - "The reduction of osmium tetraoxide is probably
by ferrocyanide. Ferrocyanide is oxidized to ferri reducing the osmium
compound."
And Walt Bobrowski, who provided the Karnovsky reference " Use of
ferrocyanide-reduced osmium tetroxide in electron microscopy". ASCB meeting New
Orleans, LA 1971:146.

Unfortunately the stakes of the wager were not so great that they could be
shared.

Best regards,
Paul Webster
Center for Cell Imaging
Yale University school of Medicine
http://info.med.yale.edu/cellimg





From: rjpalmer-at-utkux.utcc.utk.edu (Robert J. Palmer Jr.)
Date: Thu, 2 Nov 1995 07:59:42 -0500
Subject: Re: Microwave fixation, actually continued comment

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Message-Id: {9511021258.AA08939-at-utkux1.utk.edu}

Netiquette (I hate all those nerdy little "I'm a Net-insider and you're
not" things like :), :(, BTW, and IMHO) also has clear guidelines on
"flaming" (for non-nerdy Net users, this refers to complaints, particularly
specific attacks on the originator of a posting). Net etiquette also says
that the SysOp is king and it is therefore not your duty nor anyone else's
to bash people who have not used to mailing list correctly. Lastly,
experienced Net users don't make mistakes like sending replies to the list
that are meant for the original user. Take your lumps (and they are roudly
deserved as witnessed by the thread YOU generated) and let the SysOp decide
when someone has stepped over the lines. Enough already!

} OK, so Gary Login's post was not commercially based. I'm sorry. I was
} going to send the question to Nestor Zaluzec only but I got distracted and
} sent it to the list by mistake. However, I think it does bear some further
} comment and/or discussion.
} This list is getting very large and there are now many cases of messages
} that should not be sent to all subscribers without there being some
} indication that a large number of them want to see the responses. It
} really shouldnt have been posted to the list at all, and my reasoning for
} that is below.
}
} Net etiquette (or Netiquette as it is typically known ) says that if there
} is a question in a mailing list or newsgroup then the correct response is
} to send a message to the original poster. Often a poster will say "private
} replies please, if there is enough interest I will summarize to the
} net/list". This should be implicit in ALL posts. In this manner if the
} poster receives many replies, and there is much duplication in the replies,
} then any summary can be edited by the original poster before sending the
} information out to everyone on the list/net. People wishing copies of the
} replies also send requests to the original poster.
} If the original poster geets say five to ten "me too" requests for the
} information
} You may ask why should the original poster have to do this editing and
} summarizing work? Well they are getting the free info courtesy of others
} so it isnt exactly a large price to pay.
}
} Maybe these instructions should be part of the files that gets sent to new
} subscribers.
}
} } Although not from a company this looks much like a shameless commercial to
} } sell this guy's books, what do you think?
} }
} } } 1) Rapid (i.e.,in seconds) microwave fixation is used with success to fix
} } } plant
} } } and animal tissues. In addition, several reports show the benefits of using
} } } microwave fixation to preserve soft tissues encased by hard shells (e.g.,
} } } clams,
} } } teeth, insects).
} } }
} } } Recommended reading:
} } } Login, G. R., & Dvorak, A. M. (1994). Application of microwave fixation
} } } techniques in pathology to neuroscience studies: A review. J Neurosci
} } } Methods, 55, 173-182.
} } } Login, G. R., & Dvorak, A. M. (1994). Methods of microwave fixation for
}
} John Mansfield
} North Campus Electron Microbeam Analysis Laboratory
} 413 SRB, University of Michigan
} 2455 Hayward, Ann Arbor MI 48109-2143
} Phone: (313)936-3352 FAX (313)936-3352
} jfmjfm-at-engin.umich.edu, John.F.Mansfield-at-umich.edu
} or jfmjfm-at-umich.edu they all reach me!
} URL: http://wwwpersonal.engin.umich.edu/~jfmjfm/jfmjfm.html





From: Richard E. Edelmann :      EDELMARE-at-CASMAIL.MUOHIO.EDU
Date: Thu, 2 Nov 1995 08:46:20 -500
Subject: PAL Laserdisk to anything

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Technically not a microscopy question but an imaging question.

Does anyone out there know of any source for converting a european
PAL format laserdisk (of microscopic images and audio) into any
common North American format (i.e. NTSC Video tape or CD-ROM) so we
can use the disk as a teaching tool? (The company that sells the
disk does not offer it in NTSC or have the ability of converting it).





From: baskin-at-biosci.mbp.missouri.edu (Tobias Baskin)
Date: Thu, 2 Nov 1995 08:56:28 -0600
Subject: Re: Microwave fixation, actually continued comment

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Message-Id: {v01520d00acbe887777ce-at-[128.206.15.200]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Greetings,
John Mansfield wrote:
} OK, so Gary Login's post was not commercially based. I'm sorry. I was
} going to send the question to Nestor Zaluzec only but I got distracted and
} sent it to the list by mistake. However, I think it does bear some further
} comment and/or discussion.
} This list is getting very large and there are now many cases of messages
} that should not be sent to all subscribers without there being some
} indication that a large number of them want to see the responses. It
} really shouldnt have been posted to the list at all, and my reasoning for
} that is below.
}
} Net etiquette (or Netiquette as it is typically known ) says that if there
} is a question in a mailing list or newsgroup then the correct response is
} to send a message to the original poster. ...

I disagree completely. THere is no such thing as ONE and only one
proper response. Instead, there are many kinds of responses. Although some
responses are likely to be really speciallized, many other responses are
likely to be of interest to many more than the poster. In the case in
point, its obvious from the postings that microwave fixation is of concern
to many, even in passing. It's much more efficient, spontaneous and
interesting for the responses of a general nature to be posted directly to
the group. They all have subject lines. IF you don't want to read a post
about microwave fixation-hit the delete key.

Just my two cents,
Tobias Baskin



- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
___ ____ ^ ____ _____ Tobias I. Baskin
/ \ / / \ / \ / University of Missouri
/ | / / \ / / Biological Sciences
/___ / /__ /_____\ / /__ 109 Tucker Hall
/ / / \ ( / Columbia, MO 65211 USA
/ / / \ \ / voice: 314-882-0173
/ /____ / \ \____/ /_____ fax: 314-882-0123






From: Daniel L. Callahan :      dlc-at-owlnet.rice.edu
Date: Thu, 2 Nov 1995 09:52:40 -0600 (CST)
Subject: 50 nanometer resolution standard

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All:

I've had a recent request for a resolution standard in the 50-100 nm range.
I believe that this is for a relatively low-resolution SEMPA system, but
could be mistaken. This seems to be awkwardly between the {5 nm
standards used for modern SAM resolution and a good optical microscope
standard. I was hoping that someone either might have an inspiring
though or perhaps an idea from the days when SEM resolution wasn't quite
what it is today?

Please respond directly; if anyone expresses interest I will summarize
responses.

Daniel L. Callahan
Department of Mechanical Engg. and Materials Science
Rice University
dlc-at-owlnet.rice.edu




From: Daniel L. Callahan :      dlc-at-owlnet.rice.edu
Date: Thu, 2 Nov 1995 09:52:40 -0600 (CST)
Subject: 50 nanometer resolution standard

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All:

I've had a recent request for a resolution standard in the 50-100 nm range.
I believe that this is for a relatively low-resolution SEMPA system, but
could be mistaken. This seems to be awkwardly between the {5 nm
standards used for modern SAM resolution and a good optical microscope
standard. I was hoping that someone either might have an inspiring
though or perhaps an idea from the days when SEM resolution wasn't quite
what it is today?

Please respond directly; if anyone expresses interest I will summarize
responses.

Daniel L. Callahan
Department of Mechanical Engg. and Materials Science
Rice University
dlc-at-owlnet.rice.edu




From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Thu, 02 Nov 1995 10:36:59 -0600
Subject: Re: Microwave fixation, actually continued comment

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Message-Id: {199511021539.JAA26587-at-bcm.tmc.edu}
X-Sender: joiner-at-bcm.tmc.edu
X-Mailer: Windows Eudora Version 2.1.1
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

At 11:34 PM 11/1/95 -0400, John F. Mansfield wrote:

} Net etiquette (or Netiquette as it is typically known says that if there
} is a question in a mailing list or newsgroup then the correct response is
} to send a message to the original poster. Often a poster will say "......
I will summarize to the net/list....."
*************

It seems to me that your approach would hamper the free exchange that this
list is so good at and would be a lot more work than merely erasing the
superfluous replies; it's not that difficult. People seem to be pretty
skilled at crafting their SUBJECT: headings so you don't have to read
everything posted.
* * Joiner Cartwright, Jr. * *





From: William Tivol :      tivol-at-wadsworth.org
Date: Thu, 2 Nov 1995 13:31:01 -0500 (EST)
Subject: Re: Structure info from XRD -- amorphous material

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} If you
} are getting fringe contrast in the TEM, then the particles are not amorphous.
}
Dear Scott,
Are the fringes observed when an opaque material with a flat edge
occludes a light source the same as those seen in the EM at the edge of a
particle? If so, then the crystallinity of the particle has no bearing on
whether the fringes are there. I think all that's required is an abrupt
change in mass density (or opacity).
Yours,
Bill Tivol




From: William Tivol :      tivol-at-wadsworth.org
Date: Thu, 2 Nov 1995 13:44:04 -0500 (EST)
Subject: Re: Reduced Osmium

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}
} Dear colleagues,
} can someone help by providing a good recipe for reducing osmium tetroxide to
} help increase contrast in epoxy resin - embedded biological material? Is
} potassium ferri-, or ferro- cyanide the best? I thought I knew the answer but a
} post-doc here has given me doubts so we have a small wager riding on this.
}
Dear Paul,
To reduce osmium, one must oxidise the other reagent, so ferri (Fe2+)
should be better than ferro (Fe3+).
Yours,
Bill Tivol




From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Thu, 02 Nov 1995 14:13:49 -0600
Subject: Re: PAL Laserdisk to anything

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Message-Id: {199511021916.NAA19306-at-bcm.tmc.edu}
X-Sender: joiner-at-bcm.tmc.edu
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Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

At 08:46 AM 11/2/95 -500, Richard Edelmann wrote:
} Technically not a microscopy question but an imaging question.
}
} Does anyone out there know of any source for converting a european
} PAL format laserdisk (of microscopic images and audio) into any
} common North American format (i.e. NTSC Video tape or CD-ROM) so we
} can use the disk as a teaching tool? (The company that sells the
} disk does not offer it in NTSC or have the ability of converting it).
}
}
**************
Richard -

Look in your yellow pages under { {video - tape duplicating & transfer
services} } . You should find one or more, depending on the size of your
community, parties who can help you.


Joiner Cartwright, Jr., Ph.D.
Director, Electron Microscopy tel.: (713)798-4658
Department of Pathology, Rm.286-A FAX: (713)798-3945
Baylor College of Medicine Internet: joiner-at-bcm.tmc.edu
One Baylor Plaza Compuserve: 71555,1206
Houston, Texas 77030 U.S.A.






From: trenkler-at-imec.be
Date: Thu, 2 Nov 1995 21:46:51 +0100
Subject: 8.1 Different Video Norms!

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extract from faq-at-soc.cult.german
I know, it's not about laser disks, but give it a try!



PAL format videotapes (as used in Germany) will not display properly
using an NTSC (used in, eg, USA) based VCR and vice-versa.

There are services where video conversion from any format to any other
format can be made for a fee (VHS, VHS-C and 8 mm types of cassettes.)
This will allow playback of videotapes made overseas using US TV's and
VCR's (PAL, SECAM -} NTSC) and vice-versa (NTSC -} PAL, SECAM,
etc ...)

It is also not too expensive to get a VCR which is able to **play**
NTSC and PAL tapes.
Only a few VCR's are able to **record** and play VHS tapes in NTSC and PAL
(e.g. Panasonic AG-W1, about DM 5000). Cheaper VCR's are able to play
different formats (NTSC, PAL, SECAM).

**Do it yourself**

With these setups you can transfer from NTSC to/from PAL at reasonable cost.
Don't expect studio quality though:
o Akai VS R110EM is a three system unit - PAL, NTSC, SECAM , costs
about US$200 mailorder (smile video, nyc).
o AKAI VSX-560, *HiFi-Stereo*, tuner, features include NTSC
playback on PAL TV, US$500 (mailorder from 47th St Photo)
o AIWA MG360S also 3 systems, costs about US$450 (mail order,
j/R music world, nyc, 1 800 221 8180)
o Another VCR that is "reasonably" priced is sold by Radio-Shack. The
VCR is available through special order only; and not all Radio Shack
employees know that this machine even exists. If they don't, have
them look in the current catalog for #16-706. The cost is US$600.
(You'll need a second VCR for conversions.)
[3/94]

**Commercial conversion**

o International Video Conversion
520 Harvest Lane,
Raleigh, NC 27606-2217,
tel (919) 233-8689

Fees: US$25 + 5 S&H,
Price of a High Grade Cassette Included, 2hrs or less.
Delivery: Mailed back the next day, express shipping at request.
Payment: Check, Cash or Money Order mailed with tape.

o sasjrm-at-unx.sas.com does it for US$5 per hour + US$3 for the blank tape.
Formats: NTSC, PAL, NPAL, MPAL, SECAM, MSECAM

o Soffel VDO
2250 Monroe St #263,
Santa Clara, CA 95050,
tel (408) 985 2098

US$20 per tape (up to 2h, each add. hour US$10). Tape, S&H included.
Mail only, next day shipping, overnight available. Check, cash,
money order. Does: NTSC (8mm, Hi8, VHS) -} PAL (VHS)

o Give your local shops a try! I found a **Camera Shop** that does
PAL {-} NTCS conversions; a bit expensive, though (US$20/h).
But if you need something the very next day...
[1/94]




From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Fri, 3 Nov 1995 10:00:09 GMT+1200
Subject: Netiquette a la John Mansfield

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I disagree with John's view that replies should be routinely posted
privately. I learn a lot by scanning the replies, often when I've not
even noticed the original query.
Provided that a subject field is entered, it's very easy just to
delete items which one doesn't want to read.

Ritchie

Ritchie Sims phone: 61 9 3737599 ext 7713
Department of Geology fax: 61 9 3737435
University of Auckland
Private Bag 92019
Auckland
New Zealand




From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Thu, 02 Nov 1995 16:43:57 -0600
Subject: Re: 50 nanometer resolution standard

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Message-Id: {199511022146.PAA03530-at-bcm.tmc.edu}
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Mime-Version: 1.0
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We're interested, man. Share it.
************


At 09:52 AM 11/2/95 -0600, you wrote:
}
} All:
}
} I've had a recent request for a resolution standard in the 50-100 nm range.
} I believe that this is for a relatively low-resolution SEMPA system, but
} could be mistaken. This seems to be awkwardly between the {5 nm
} standards used for modern SAM resolution and a good optical microscope
} standard. I was hoping that someone either might have an inspiring
} though or perhaps an idea from the days when SEM resolution wasn't quite
} what it is today?
}
} Please respond directly; if anyone expresses interest I will summarize
} responses.
}
} Daniel L. Callahan
} Department of Mechanical Engg. and Materials Science
} Rice University
} dlc-at-owlnet.rice.edu
}
}





From: MelanieOwl-at-aol.com
Date: Thu, 2 Nov 1995 18:34:20 -0500
Subject: MAP Source Request

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I am also interested in locating a source of mussel adhesive protein. If
anyone knows of a source, please post it on the list, since I think others
might be interested.
Melanie Behrens




From: gwe-at-biotech.ufl.edu (Greg Erdos)
Date: Thu, 2 Nov 1995 14:02:49 GMT
Subject: Re: Microwave fixation, actually continued comment

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I'm with you, Baskin! Better too many choices than too few. I like reading
all the replies and drawing my own conclusions filtered through my biases
rather than some one else's


} } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } }



} Greetings,
} John Mansfield wrote:
} } OK, so Gary Login's post was not commercially based. I'm sorry. I was
} } going to send the question to Nestor Zaluzec only but I got distracted and
} } sent it to the list by mistake. However, I think it does bear some further
} } comment and/or discussion.
} } This list is getting very large and there are now many cases of messages
} } that should not be sent to all subscribers without there being some
} } indication that a large number of them want to see the responses. It
} } really shouldnt have been posted to the list at all, and my reasoning for
} } that is below.
} }
} } Net etiquette (or Netiquette as it is typically known ) says that if there
} } is a question in a mailing list or newsgroup then the correct response is
} } to send a message to the original poster. ...
}
} I disagree completely. THere is no such thing as ONE and only one
} proper response. Instead, there are many kinds of responses. Although some
} responses are likely to be really speciallized, many other responses are
} likely to be of interest to many more than the poster. In the case in
} point, its obvious from the postings that microwave fixation is of concern
} to many, even in passing. It's much more efficient, spontaneous and
} interesting for the responses of a general nature to be posted directly to
} the group. They all have subject lines. IF you don't want to read a post
} about microwave fixation-hit the delete key.
}
} Just my two cents,
} Tobias Baskin
}
}
}
} - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
} ___ ____ ^ ____ _____ Tobias I. Baskin
} / \ / / \ / \ / University of Missouri
} / | / / \ / / Biological Sciences
} /___ / /__ /_____\ / /__ 109 Tucker Hall
} / / / \ ( / Columbia, MO 65211 USA
} / / / \ \ / voice: 314-882-0173
} / /____ / \ \____/ /_____ fax: 314-882-0123
}
}
}
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Greg Erdos Phone: 904-392-1295
Scientific Director,
ICBR Electron Microscopy Core Lab
218 Carr Hall Fax 904-846-0251
University of Florida E-mail: gwe-at-biotech.ufl.edu
Gainesville, FL 32611
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-





From: IAN HALLETT :      ihallett-at-MARCCRI.MARC.CRI.NZ
Date: Fri, 3 Nov 1995 09:13:50 GMT+1200
Subject: Re: Microwave fixation, actually continued comment

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In reply to John Mansfields remarks

It may be Netiquette to send replies only to the originator of the
question but I for one would find the value of the mailing list
greatly reduced if I only saw questions not answers. I value the
broad range of topics discussed and in a number of cases the
answers to relatively simple questions have made me look at
our current techniques. Also without the answers on the list how can
we get the often very stimulating discussion between different
contributors. (This also ignores the problem that many questioners no
matter how well meaning will fail to post a summary on the list).

As far as time goes I fine that it only takes me three or four
minutes to sort out potentially useful items, particularly if good
subject headings used. It would take much longer to send
individual messages for summaries of questions I am interested in.

My vote for what its worth is to keep the discussions open and active

Ian

} OK, so Gary Login's post was not commercially based. I'm sorry. I was
} going to send the question to Nestor Zaluzec only but I got distracted and
} sent it to the list by mistake. However, I think it does bear some further
} comment and/or discussion.
} This list is getting very large and there are now many cases of messages
} that should not be sent to all subscribers without there being some
} indication that a large number of them want to see the responses. It
} really shouldnt have been posted to the list at all, and my reasoning for
} that is below.
}
} Net etiquette (or Netiquette as it is typically known ) says that if there
} is a question in a mailing list or newsgroup then the correct response is
} to send a message to the original poster. Often a poster will say "private
} replies please, if there is enough interest I will summarize to the
} net/list". This should be implicit in ALL posts. In this manner if the
} poster receives many replies, and there is much duplication in the replies,
} then any summary can be edited by the original poster before sending the
} information out to everyone on the list/net. People wishing copies of the
} replies also send requests to the original poster.
} If the original poster geets say five to ten "me too" requests for the
} information
} You may ask why should the original poster have to do this editing and
} summarizing work? Well they are getting the free info courtesy of others
} so it isnt exactly a large price to pay.
}
} Maybe these instructions should be part of the files that gets sent to new
} subscribers.


Ian Hallett
HortResearch
Mt Albert Research Centre
Private Bag 92 169
Auckland, New Zealand
Fax 64-9-815 4201
Telephone 64-9-849 3660




From: Lcapel-at-eliovac.com.ar
Date: Fri, 3 Nov 1995 08:54:23 +0000
Subject: unsubscribe

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unsubscribe Lcapel-at-eliovac.com.ar






From: ldm2-at-apollo.numis.nwu.edu (L.D.Marks)
Date: Fri, 3 Nov 1995 08:02:22 -0600
Subject: Netiquette

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I think that there is a lot of truth to both sides of this
issue. One point that I would like to make is that sometimes responses
to (say) a commercial posting are worse than the original! On a few
newsgroups (not this one) I have recently seen } 30 responses to a
posting with a subject header:

"WOW! This REALLY works! Download it NOW! - money.zip"

The responses to this email (which apparently was } 7000 lines wrong)
are now more annoying than the original !




From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Fri, 03 Nov 1995 10:27:10 -0600
Subject: Netiquette, schmetiquette!

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Message-Id: {199511031529.JAA00412-at-bcm.tmc.edu}
X-Sender: joiner-at-bcm.tmc.edu
X-Mailer: Windows Eudora Version 2.1.1
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Why don't we drop the nerdy little cyberjargon? After all we on this
listserver are all technogurus of the highest order and can afford to speak
English, or other real language, aren't we?

Joiner Cartwright, Jr.


P.S. Of course, you all do realize that I hava a bigger hard disk than rest
of you compuwimps, don't you?





From: keller-at-boulder.nist.gov (Bob Keller)
Date: Fri, 3 Nov 1995 09:07:59 -0700
Subject: inv. pole fig. software

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Does anyone know of any Macintosh apps. which can be used for plotting
orientation matrices/Euler angles (from backscatter Kikuchi diffraction
patterns) into inverse pole figures? I can do the regular pole figures
already using BKD4.4 by Stuart Wright, but wish to see if any trends become
more apparent in the inverse figs. I'm aware of the software available
from TexSEM Labs for Windows and SGI, as well as popLA (for IBM compats.)
from Los Alamos, but would prefer something for the Mac.

Although most of us would like to see replies to the listserver, I'm sure
many will come directly to me. So I will post a summary if there is
interest.

Thanks!

Bob Keller
NIST Materials Reliability Div.
Boulder, CO






From: jfmjfm-at-umich.edu (John F. Mansfield)
Date: Fri, 3 Nov 1995 22:50:39 -0400
Subject: Re: Netiquette a la John Mansfield

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X-Sender: jfmjfm-at-srvr5.engin.umich.edu
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Now, if all of you had followed the rules, then I would have been able to
post a concise summary saying that I had 12 replies to my message about
this and 10 of them were in disagreement and two were in agreement and so
it seems that on this list Netiquette is out the window. And we have our
usual free-for-all. Sorry, since we should be democratic I guess I'll bow
to the majority:-).


BTW. These were not Netiquette a la John Mansfield, rules they were
Internet rules formulated in the early days of the net.

John Mansfield
North Campus Electron Microbeam Analysis Laboratory
413 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (313)936-3352 FAX (313)936-3352
jfmjfm-at-engin.umich.edu, John.F.Mansfield-at-umich.edu
or jfmjfm-at-umich.edu they all reach me!
URL: http://wwwpersonal.engin.umich.edu/~jfmjfm/jfmjfm.html






From: bozzola-at-siu.edu (John. J. Bozzola)
Date: Sat, 4 Nov 1995 03:33:47 -0600
Subject: Re: Netiquette, schmetiquette!

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I agree with Joiner Cartwright, Jr. that we should avoid jargon whenever
possible. It takes a few more key strokes but it will be better
communicated to a larger audience. OR, if there are certain well
established abbreviations, perhaps they could be posted in list of FAQ's
(frequently asked questions). Now, about that hard disk .....

} } } } } } } } } } } } } } } } } }

} Why don't we drop the nerdy little cyberjargon? After all we on this
} listserver are all technogurus of the highest order and can afford to speak
} English, or other real language, aren't we?
}
} Joiner Cartwright, Jr.
}
}
} P.S. Of course, you all do realize that I hava a bigger hard disk than rest
} of you compuwimps, don't you?


#############################################################################
John J. Bozzola, Director
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
#############################################################################






From: bozzola-at-siu.edu (John. J. Bozzola)
Date: Sat, 4 Nov 1995 03:24:25 -0600
Subject: CSMS-MIKMAS Meeting in St. Louis

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CSMS-MIKMAS PROGRAM

Friday -- November 10th, 1995
Joe Hanon's Restaurant
2430 Old Dorsett Drive
St. Louis, Mo.

This combined meeting promises to be both informative and practical with
discussions of modern, analytical technologies in light microscopy,
electron microscopy and molecular biology. Our societies have assembled
top-notch researchers and speakers in these areas. The physical setting
should be excellent and convenient to reach. Plan to attend this exciting
meeting.


9 AM Registration & introductory comments

9:30 Richard L.. Ornberg, Monsanto Company
Energy Filtered Imaging with a Light Microscope

10:30 Break --
Refreshments by Electron Microscopy Sciences

11 Jon J. McCarthy, NORAN Instruments
New and Emerging Technology for X-ray
Spectroscopy in Microanalysis

12 Lunch

1 PM Nestor Zaluzec, Argonne National Laboratory
Computers in Mcroscopy, Connectivity and
Telepresence Microscopy

3 Susan Wente, Washington University
Epitope Labeling

4 Business Meetings


HOTELS, MOTELS:

Drury Westport Inn Single rate $64.00 + tax
I-270 & Dorsett Road Includes complimentary breakfast
314-576-9966

Best Western Park Hotel Single rate $69.00 + tax
2434 Old Dorsett
314-291-8700



DIRECTIONS:

From I-70 West and East:
Turn onto I-270 south.
Exit east at Dorsett Road
Make left at first stop light.


From I-40 West and East:
Turn onto I-270 north
Exit east at Dorsett Rd.
Make left at first stop light.



#############################################################################
John J. Bozzola, Director
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
#############################################################################






From: Carmine M. Pariante :      cparian-at-emory.edu
Date: Sat, 4 Nov 1995 14:22:06 -0500 (EST)
Subject: quantitative immuno-fluorescence

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Dear collegues,

I am studying the translocation of the steroid receptor from the
cytoplasm to the nucleus after treatment with agonists, using fibroblast
L929 cells.
The steroid receptor is stained using a specific primary antibody followed
by a secondary FITC labelled antibody. To make a long story short, after the
treatment with agonists, the fluorescence in the nucleus increases, and
wityh my eyes at the microscope this effect is very evident.
Now my wish is to use the NIH image programme to quantify the fuorescence in
the cytoplasm and in the nucleus, NOT to obtain the absolute
quantification of the receptor but to demonstrate and quantify the
translocation, i.e., with different agonists.
I am using a CCD-72 series camera (DAGE-MTI) to transmit the images from the
microscope to the computer.

NOW, I HAVE TWO PROBLEMS:

- FIRST: when I play with the various control functions of the camere,
such as "black level" and "gain", both the image I see on the screen and
the pixel values change, of course. The problem is that some changes may
actually decrase the difference between quantification of fluorescence in the
cytoplasm and in the nucleus, while other increses it.
For example, the ratio between the
average pixel value in the nucleus and the average pixel value in the
cytoplasm can range from 2 to 10 IN THE SAME CELLS, just moving the control
functions (and it is not related to fading).
Can you give me any advice on how to use the control functions?

- I have also a big differences between different experiments or even
slides in the same experiment, may be due to the fact that even little
differences in the thickness of mounting media can change quantification
of fluorescence. In this case, keeping fixed the control functions, I still
can get the difference between cytoplasm and nucleus, but the
absolute average pixel values in the two compartments may vary from slides
to slide, i.e., 20 and 40 in one, 100 and 200 in other.
Do you have any advice to solve this problem??

Sorry if the questions required a lot of space

Thanks in advance

Carmine M. Pariante
Dep. of Psychiatry
Emory University School of Medicine
Atlanta, GA, USA
phone: (404)-727-8261
fax: (404)-727-3233
cparian-at-emory.edu




From: ENERGY BEAM SCIENCES, INC :      75767.640-at-CompuServe.COM
Date: 06 Nov 95 13:33:08 EST
Subject: Re: Microwave ad nauseam

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Dear colleagues:
Can we find another name for this thread, please? There might actually be folks
out there with an interest in microwave techniques, and I don't want to wade
through all this "Netiquette" stuff to find them.
Steven Slap, Energy Beam Sciences
really interested in microwaves & EM





From: ENERGY BEAM SCIENCES, INC :      75767.640-at-CompuServe.COM
Date: 06 Nov 95 14:00:37 EST
Subject: Re: SEM low resolution standards

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I am replying to the list, as I have received several "me, too" requests. There
are several options available: a gold on carbon test specimen with particle
sizes 5nm to 150nm; an aluminum-tungsten dendrite specimen, designed for use in
the 25nm-75nm range; a cross grating replica with 19.7 lines/mm; etc. Please
contact me directly for details.
Steven E. Slap, Vice President





From: MELLIOTT-at-prl.pulmonary.ubc.ca
Date: Sun, 5 Nov 1995 12:59:14 +0800PST
Subject: chromogen-green

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Dra Patricia Pons was asking for a green chromogen to develop PAP
reactions. We found one accidentally which might work. We use the
Histomark True Blue substrate and then fix the section in Bouin's
fix-the True Blue turns green. You can then was the slide in water or
buffer to get rid of the Bouin's. The one thing you have to watch is
that TrueBlue in our hands is solvent soluble. We air dry the sections
before coverslipping.

Mark Elliott,
UBC-Pulmonary Research Lab
Vancouver, BC
Canada





From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Mon, 06 Nov 1995 17:25:46 -0600
Subject: Re: Microwave ad nauseam

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Message-Id: {199511062228.QAA04132-at-bcm.tmc.edu}
X-Sender: joiner-at-bcm.tmc.edu
X-Mailer: Windows Eudora Version 2.1.1
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

At 01:33 PM 11/6/95 EST, "ENERGY BEAM SCIENCES, INC" wrote:

} Dear colleagues:
} Can we find another name for this thread, please? There might actually be
folks
} out there with an interest in microwave techniques, and I don't want to wade
} through all this "Netiquette" stuff to find them.
} Steven Slap, Energy Beam Sciences
} really interested in microwaves & EM
}
****************
It seems that maybe we have a consensus and can drop it.

* * Joiner Cartwright, Jr. * *





From: Carl Henderson :      chender-at-umich.edu
Date: Mon, 6 Nov 1995 17:10:00 -0500 (EST)
Subject: EMPA: Carbide standards

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Greetings,

We are undertaking to analyze an unusual mineral which contains Mo, As,
Ni, Fe, S and apparent C. I am aware of many of the pitfalls of
analyzing C (peak shape variability, sample cleanliness, shallow depth of
C x-ray production, etc.), but would like to find a better standard than
diamond. Can anyone point me towards some heavy element carbides
(e.g., Mo-C, Nb-C)?

Please respond via personal email; I will summarize responses to the
group if there is sufficient interest. (No, JFM did not tell me to do
this ;-) ).

Thanks in advance.

Carl

Carl Henderson
Electron Microbeam Analysis Laboratory-Central Campus
University of Michigan
2005 C.C. Little Bldg.
Ann Arbor, MI 48109-1063 USA
--------------------------------
(313) 936-1550 (voice)
(313) 763-4690 (FAX)
chender-at-umich.edu (e-mail)
--------------------------------




From: Tina Carvalho :      tina-at-halia.pbrc.Hawaii.Edu
Date: Mon, 6 Nov 1995 15:18:55 -1000 (HST)
Subject: LM: colloidal gold enhancement

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Aloha, microscopists,

Do any of you have a current favorite method for enhancement (silver or
whatever) of 5 to 10 nm colloidal gold for light microscopy? The person
who is asking will be labelling plant goop on a slide, not sections of
embedded material. And is darkfield the best way to view? I haven't
had to look for gold with LM before - I rather presume silver
enhancement would be the same as for TEM, but more...?

Thanks for any hints!

Tina

*****************************************
Tina (Weatherby) Carvalho *
Biological Electron Microscope Facility *
University of Hawaii *
(808) 956-6251 *
tina-at-ahi.pbrc.hawaii.edu *
http://www.pbrc.hawaii.edu/bemf/ *
*****************************************





From: Bren.Gannon :      Bren.Gannon-at-cc.flinders.edu.au
Date: Tue, 7 Nov 1995 12:09:34 -9.5
Subject: Mercox suppliers

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Message-Id: {9511070145.AA40653-at-gamgee.cc.flinders.edu.au}
Sender: {aybjg-at-gamgee.cc.flinders.edu.au}

Dear fellow students of small things:

Some years ago, I used to get a corrosion vascular casting resin
for SEM observation called Mercox direct from the manufacturers/
distributors in Tokyo, Japan. My stock has finally run out.

The manufacturers were origionally called Japan Vilene Ink & Chemical Coy and later
just Vilene Hospital.

However, I don't have a current address. Does anyone have an address
where I can get Mercox from now, either from Japan, or elsewhere?
Current price would also be useful if you have it.

Please send replies direct to Bren.Gannon-at-flinders.edu.au

(I will post the addresses to the list, if anyone else indicates interest)

Bren.
Bren Gannon
Microcirculation & Lymphology Lab
Anatomy & Histology Dept., Flinders Univ Medical School
GPO Box 2100, Adelaide S.A 5001 Australia
Voice (61-8)-204-4183; Fax (61-8)-277-0085
E-Mail Bren.Gannon-at-flinders.edu.au




From: zaluzec-at-Microscopy.Com (Nestor J. Zaluzec)
Date: Tue, 7 Nov 1995 00:42:39 -0600
Subject: Enough of the Etiquette Wars,--- Nestor

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G'day Colleagues....

I think it's time to draw the line. Let's get back to
Microscopy & Microanalysis. I for one have had my
fill of the last few days of postings about Net etiquette.

You should all remember when I see something that
I feel crosses the line, then I send a private note
to the author. You are all welcome to do the same,
but we don't need copies sent to everyone on the list.

I have NO problems with people responding to questions
on the listserver, that is one of our defined operating modes.
If the answer involves a product that is for sale, that is also
acceptable, so long as the answer is in direct response to a
posted question. However, I expect the individual
to be brief and to the point. If the posting becomes a commerical
then I will politely inform the author. If it goes to far, well......

Remember these postings give everyone the benefit of
OUR collective knowledge. This includes both public domain,
and commerical material. Let's face it folks
there is a lot of information out there that most of us
do not know exists, or have forgotten about.

Also , if some kind soul, volunteers to collect a group of
related messages and correlate them into one document
that is equally acceptable way of getting the information
across to this group. There are pluses and minuses to
the method. I have no preference.

Please DONOT post any agreements/disagreements
we have had far too much bandwidth on the topic.
I think all bases have been covered.


Cheers...
Nestor
Your Friendly Neighborhood SysOp






From: deschuyt-at-ccmailg1.sbbio.be (Michel DESCHUYTENEER)
Date: Tue, 7 Nov 1995 13:18:27 +0000
Subject: Postings and replies, a technical note

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Mime-Version: 1.0

Greetings everyone.

Regarding replies to queries posted to the list, the consensus appears
to be that all messages should appear on the list. I support this
view.

There is however a little technical twist.
A number of replies to any posting are "private" by default. Some
mailing systems (like the one used by my company) will send the reply
directly to the original sender, not to the list.
As a result, some queries seem to get no answer or messages appear in
reply to a reply that never showed up on the list in the first place.

Such a system is easy to spot: it will usually display the original
sender's address in the mailbox directory and in any case put the
original sender's address in the "To:" field when a REPLY command is
issued.
If you are in this case, either replace the address with that of the
list in the "To:" field or add a CC: to the list when replying. Also,
in some cases a configuration change may do the trick permanently.
Check your local SysOp for details.

Regards,
MICHEL


Michel Deschuyteneer deschuyt-at-sbbio.be
Scientist - Electron Microscopy Laboratory
SmithKline Beecham Biologicals
Rue de l'Institut, 89 - B1330 Rixensart, BELGIUM
Tel: +32-2-656 9290 Fax: +32-2-656 8113





From: Lawrence Kordon :      nikon-at-jagunet.com
Date: Tue, 7 Nov 1995 08:55:18 -0500 (EST)
Subject: Re: LM Colloidal Gold

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Tina,

In light microscopy, Colloidal Gold as well as Silver grain stains are
best viewed in Reflected Polarized light. This requires an
Epi-Illuminator with a Polarizer and Analyzer crossed to one another.
If your microscope is equipped with a Fluorescence Illuminator you just
need a set of filters, with a half-mirror (50%) in place of the
Dichroic mirror and polarizer and analyzer replacing the exciter and
barriers respectively. While Darkfield will identify stained regions,
polarized light is most suitable.

Good Luck!

Lawrence Kordon
Nikon, Inc.
Columbia, Maryland (GO BALTIMORE BROWN!)




From: Lawrence Kordon :      nikon-at-jagunet.com
Date: Tue, 7 Nov 1995 08:55:18 -0500 (EST)
Subject: Re: LM Colloidal Gold

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Tina,

In light microscopy, Colloidal Gold as well as Silver grain stains are
best viewed in Reflected Polarized light. This requires an
Epi-Illuminator with a Polarizer and Analyzer crossed to one another.
If your microscope is equipped with a Fluorescence Illuminator you just
need a set of filters, with a half-mirror (50%) in place of the
Dichroic mirror and polarizer and analyzer replacing the exciter and
barriers respectively. While Darkfield will identify stained regions,
polarized light is most suitable.

Good Luck!

Lawrence Kordon
Nikon, Inc.
Columbia, Maryland (GO BALTIMORE BROWN!)




From: luciom-at-newton.umsl.edu (lucio MuleStagno)
Date: Tue, 07 Nov 1995 09:23:50 -0600
Subject: Ion - Mill needed

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Hi,
I know someone in Europe who is urgently in need of an Ion - Mill. No
particular preference for model or type. If you know of an available used
mill could you please let me know ?

Thanks

lucio
***********************************************************************
Lucio Mule'Stagno
Physics Dept & Center for molecular electronics
Univ. of Missouri- St.Louis
tel 314 - 516 5933
e- mail LUCIOM-at-NEWTON.UMSL.EDU

MEMC Electronic Materials Inc.,
Materials Technology Grp.,
St.Peters,MO
tel 314 279-5000 ext 2315
fax 314 516 5157
e-mail LMULESTAGNO-at-MEMC.COM
************************************************************************

"...if you can judge a wise man by the color of his skin, than mister you're
a better man than I .. " Aerosmith
*************************************************************************





From: houtsmuller-at-pa1.fgg.eur.nl (Adriaan Houtsmuller)
Date: Tue, 7 Nov 1995 15:04:28 +0100
Subject: RE: Netiquette a la John Mansfield

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Ritchie Sims wrote:

I disagree with John's view that replies should be routinely posted
privately. I learn a lot by scanning the replies, often when I've not
even noticed the original query.
Provided that a subject field is entered, it's very easy just to
delete items which one doesn't want to read.

Ritchie

Ritchie Sims phone: 61 9 3737599 ext 7713
Department of Geology fax: 61 9 3737435
University of Auckland
Private Bag 92019
Auckland
New Zealand

I fully agree with mr. Sims, especially with what he states about the
subject field. Possibly it is even better if every replier uses (exactly)
the same subject description (RE: {original subject header} ), so that the
mail sorter can sort them together (and they can be deleted together if not
of interest).

Adriaan Houtsmuller


* Adriaan Houtsmuller,
* Research School for Pathophysiology
* of Growth and Differentiation,
* Department of Pathology,
* Erasmus University Rotterdam (EUR),
* P.O. BOX 1738,
* 3000 DR Rotterdam,
* The Netherlands,
* Phone +31 10 408 7499
* Fax +31 10 436 6660






From: ENERGY BEAM SCIENCES, INC :      75767.640-at-CompuServe.COM
Date: 07 Nov 95 12:02:42 EST
Subject: Re: carbide standards

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We have been able to locate two standard materials which may work:
MO2C, available as a powder (44um particles or smaller)
NbC, available as a fine powder (5um particles approx)
Please contact me directly for more information.
Best regards
Steven Slap, Vice-President





From: Buddy Steffens :      STEFFENS.B-at-calc.vet.uga.edu
Date: Tue, 7 Nov 1995 09:07:13 EST
Subject: Mercox

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Regarding Mercox resin:

This was always available from Ladd...however I'm not sure if they
are still in business.

Several years ago, in an attempt to find a lower cost alternative to
Mercox, I experimented with some of the acrylic casting resins that
are available at hobby shops. These are for making casts of
interesting objects, insects, etc. For my purpose (rat carotid),
they worked well. Unfortunately, I can't recall the name of the
products that I tried. I do remember though that they were but a
fraction of the cost of Mercox.
Hope this is of some use.
======W. L. Steffens, Ph.D========
==Dept. of Veterinary Pathology===
==College of Veterinary Medicine==
======University of Georgia=======
=========Athens, GA 30602=========
====STEFFENS.B-at-CALC.VET.UGA.EDU===
=======Voice: (706) 542-5536======
========FAX: (706) 542=5828======




From: Paul Webster :      Paul.Webster-at-quickmail.yale.edu
Date: 7 Nov 1995 12:09:02 -0400
Subject: Re: LM colloidal gold

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Message-Id: {n1396376261.20872-at-QuickMail.Yale.edu}

Although eip-polarized light is the fastest way to look directly at gold labeled
sections it is certainly not the most economical if you are not fully equipped
to begin with.

SIlver enhancement is a simple alternative and works the same as when used for
EM. The silver solution is reacted with the gold and then fixed using a
solution similar to photographic fixers. As there is no worry about grain size
any mordanting steps can be omitted. A simple kit which has worked well for LM
in my hands (J. Cell Biol. 1988 106:279-288) is a kit from Amersham called
"IntenSe". The silver solution is not light sensitive so it is possible to
check the cells or sections with the light microscope before fixing, repeating
the reaction until a signal is detected. Detection is easy using transmitted
light or phase contrast, the signal appears as black precipitates.

Another alternative is to use enzyme histochemistry, where the signal is
detected as a colored reaction product (HRP, Alk.Phos. etc).

I have placed a small discussion on this subject on the WWW - URL
http://info.med.yale.edu/cellimg/Cryo-LM.html if you are interested.





From: ENERGY BEAM SCIENCES, INC :      75767.640-at-CompuServe.COM
Date: 07 Nov 95 08:16:56 EST
Subject: Re: LM: colloidal gold enhancement

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The standard procedure for enhancing colloidal gold particles with a silver
enhancement kit is:
1) Wash section very thoroughly with distilled or deionized water
2) Mix 3 drops each of initiating solution and enhancing solution in a clean
test tube
3) Add several drops of the mixture to the slide and monitor development of
silver stain periodically under the microscope at room temperature. Do not
expose slide to high intensity light for prolonged periods. Staining is
typically complete when stained areas have a brown-black stain. Stop the
reaction before non-specific background appears by thorough washings in
distilled water.
4) To stop the reaction, wash thoroughly with water. If development is not
complete, use fresh solutions and continue silver enhancement. Counterstain as
desired.

Note that this procedure was written for our own BioSite silver enhancement kit,
but should be applicable to most high quality commercially available kits.

These instructions can be found at our WWW site as part of a complete
instruction brochure on use of BioSite colloidal gold reagents.
(http://www.mwrn.com/ebs/ebs.htm)
Steven E. Slap, Vice-President





From: jlafeber-at-fe3.rust.net (John Lafeber)
Date: Tue, 7 Nov 1995 16:14:51 -0800
Subject: Developing Depth of Focus Multiplier

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Tardis, Los Alamos, New Mexico was asked by a client to combine
a stack of images each with a limited depth of focus into one
image that shows all areas in focus. They did a very nice job
and I am encouraging them to add the interface and software that
would make this a universal tool.

This is where you come in, is there appreciable interest in
something like this?

Another thing of course is can it be made universal and easy to
use? Are there applications where it doesn't work? We need
feedback and image sets to drive this project forward. The Alpha
image set and result can be seen at:

http://www.rust.net/~jlafeber

From what I have seen of image sets (six) at this time the
Tardis approach is robust and very, very fast (under 1 minute
with a standard Pentium PC).

Please let me know what you think.






From: MSCROGGIE-at-TRENTU.CA
Date: Tue, 07 Nov 1995 16:58:10 -0400 (EDT)
Subject: cadmium staining

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I am seeking a fixation protocol and staining procedure for cadmium in
embryos for the TEM. I am very interested in the histochemical staining
procedure and protocol using Benzothiazolylazonapthol derivatives (BTAP's)
synthesized by Sumi Y, Muraki T, and Suzuki T (1979, 1980, 1982). BTAP's
stain differentially stain cadmium in tissues embedded in paraffin. Any
help would be appreciated. Thank-you. MScroggie , university student, Trent
University. MSCROGGIE-at-TRENTU.CA




From: dianavd-at-eye.usyd.edu.au (Diana van Driel)
Date: Wed, 8 Nov 1995 10:33:15 +1100
Subject: Re: LM: colloidal gold enhancement

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Message-ID: {FD5A9F3001F70300-at-mhs.unc.edu}
In-Reply-To: {30079E3001F70300}

} Aloha, microscopists,
}
} Do any of you have a current favorite method for enhancement (silver or
} whatever) of 5 to 10 nm colloidal gold for light microscopy?

I routinely enhance labelled wholemounts of retina (300 mic thick) with
BioCell (UK) silver enhancing kit. Wash labelled, fixed tissue with water
before and after enhancing; enhance till visible under LM. View normally,
with tissue mounted under glycerol, as dehydrating seems to turn everything
black. It is also more sensitive if you use 1nm gold conjugate as the
initial label (also available from BioCell). Darkfield is more sensitive,
but normal LM is fine and you can see the rest of the tissue.

Diana van Driel
Dept Ophthalmology
Sydney University
Sydney 2006
Australia






From: Owen P. Mills :      opmills-at-mtu.edu
Date: Tue, 7 Nov 1995 17:38:09 -0500
Subject: Sample prep - polymer nanoparticles

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Hello;

I'm working with a group that wishes to make morphological measurements on
polymer nanoparticles using electron microscopy (SEM or TEM). The
nanoparticles will likely be polyvinylpyridine, polycyanoacrylate, possibly
polymethylmethacrylate. The nanoparticles should be in the range of
100-200nm, but they may well be bigger. They would like to see the whole
range of particles and be able to report average particle diameter and the
whole range of particle sizes (size polydispersity).

Previous attempts, by placing drops of particles suspended a solvent on a
SEM mount or TEM grid, have resulted in "clumps" of particles that are
difficult to measure.

Can anyone recommend a prodecure that might work for this situation. I have
heard of (but am unfamiliar with) aerosol procedures, will that work?

Also, can anyone recommend a few useful general references on electron
microscopy of polymers. I have "Polymer Microscopy" by L. Sawyer & D. Grubb
on order now.

Thanks.
Owen P. Mills
Michigan Technological University
Metallurgical & Materials Engineering
Rm 512 MME Building
Houghton, MI 49931
906-487-2002
906-487-2934 FAX
opmills-at-mtu.edu





From: Morgan Donald J :      3djm29-at-qlink.queensu.ca
Date: Tue, 7 Nov 1995 21:27:42 -0500 (EST)
Subject: High Resolution Microanalysis

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I am working on an undergraduate thesis to improve the resolution of xray
detectors for EDS systems, likely using a cryogenic detector. Our
goal is to achieve a resolution of about 10eV (for Mn K alpha), while
retaining reasonable count rates (allowing faster processing then
wavelength dispersive spectrometry). I would be interested in hearing
about specific applications for such a system (such as trace element
analysis or light element detection) and about
the specific shortfalls of current EDS systems with less resolution
(such as two peaks that you would really like to resolve but can't). If
convenient, any references to literature would be appreciated.

Thank you for your time.

Don Morgan
Queen's University
Kingston, ON
(613) 530-3506




From: Kevin Schram :      schra001-at-coyote.csusm.edu
Date: Tue, 7 Nov 1995 20:48:43 -0800 (PST)
Subject: SEM Protocol for Insect Eyes

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Heh all!

Anyone got a protocol for the preparation of the compound eyes of insects
(Drosophila) for scanning electron microscopy?

If so, would you be willing to share it?

Contact me at:

schra001-at-coyote.csusm.edu


Thanks in advance!




From: zaluzec-at-Microscopy.Com (Nestor J. Zaluzec)
Date: Tue, 7 Nov 1995 23:01:20 -0600
Subject: : High Resolution EDS Detectors for Microanalysis

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Don Morgan asked........

} I am working on an undergraduate thesis to improve the resolution of xray
} detectors for EDS systems,.................. references appreciated..



Don,

You should get a copy of the Proceedings of the 29th Annual
Conference of the MicroBeam Analysis Society, held in BreckenRidge
Co, Aug. 6-11, 1995. Editor: E. S. Etz by VCH Publishers.

There were several papers on the topic of ultra high energy resolution
EDS detectors there, and plenty of references in the papers therein.

You might also consider alternate means of parallel detection of x-rays.
such as combining multilayer x-ray optics and multichannel position
sensitive detectors and/or CCD arrays. It brings in a totally different
bag of worms, but nevertheless is an alternate approach.

You should for example look up the work of Dave Wittry and colleagues. Try
in the J. of Applied Phys. ~ 1990-1994 there are several papers by him
on the topic of PolyChromatic Diffraction Spectrometers (i.e. Parallel
detectors for X-ray Microanalysis). as well as one in Ultramicroscopy
circa 1988 or 89 entitled "Two Dimensional CCD Arrays as Parallel Detectors
in Electron Energy Loss and X-Ray Wavelength Dispersive Spectroscopy" by
Strauss & Zaluzec (sorry but I don't have the exact reference on that one
handy).


Remember it is not just energy resolution that is the important factor.
You also need good geometrical collection efficiency, small size (so that
your detector will fit in your microanalysis instrument) and
the ultimate controlling factor a reasonable price tag.

Cheers... Nestor
Your Friendly Neighborhood SysOp









From: R.G.White-at-sci.monash.edu.au (Rosemary White)
Date: Wed, 08 Nov 1995 16:49:59 +1200
Subject: mounting media

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Dear all,

While in Germany a few years ago, a colleague bought a xylene-based medium
called Melanol for making permanent (or at least semi-permanent) mounts.
She has just about run out but can't find a supplier to buy more. Is there
a new supplier of this mountant? If not, can anyone advise re. a suitable,
less toxic substitute?

TIA,
Rosemary White



____________________________________________________________
Rosemary White __ /
Department of Ecology _/ \__/ \
and Evolutionary Biology / \
Monash University / Australia \
Clayton, Victoria 3168 \ ____ /
phone 61-3-9905 5670 \_/ \_*_/
fax 61-3-9905 5613 __
email r.g.white-at-sci.monash.edu.au \/





From: dietmar.reiter-at-uibk.ac.at (Dietmar Reiter)
Date: Wed, 8 Nov 1995 13:52:13 +0100
Subject: Microscopical demo-images site?

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Dear Fellow Microscopists,
I=B4m looking for EM micrographs of periodical (biological) objects
(particles, macro molecules), suitable for course introduction to FFT
and/or electron diffraction). Is there any internet (www, ftp) source for
such demo-images?

Thanks for any response, -Dietmar-

+++ Dietmar Reiter {dietmar.reiter-at-uibk.ac.at} ++++++++++++++++
+++ Dept. of Zoology and Limnology, University of Innsbruck ++++
+++ Technikerstrasse 25, A - 6020 Innsbruck, Austria +++++++++
+++ ph: (+43)-512-507-6170 (-6161), fax: ..-507-2930 (-2957) +++

... If I would have had more time,
I would have written a shorter e-mail ...






From: George.C.Ruben-at-Dartmouth.EDU (George C. Ruben)
Date: 08 Nov 95 07:25:27 EST
Subject: Re: Sample prep - polymer nanoparticles

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Message-id: {6612461-at-prancer.Dartmouth.EDU}

Dear Owen,

A paper
["Quantification of particle sizes with metal replication under standard
freeze-etching conditions: a gold ball standard for calibrating shadow widths
was used to measure freeze-etched globular proteins" Microscopy research and
Technique 32 (1995) 312-329.] was just published that employs 45deg.
unidirectional Pt-C replication to measure particles. This paper will give you
insight into the problems of replication and how to avoid the pitfalls---- at
the end of the paper is a vertical replication method which (Fig. 17b) is the
most powerful and reliable of the replication methods. This method employs a
small metal coating correction to achieve the actual particle size (see ref in
paper). This method would allow you to measure the asymmetry of the particles
as well.
Another method which is more readily available to you but can not help
you in the 1-2 nm size range is a negative staining technique. For this method
you would need to make up a 2% solution of Uranyl acetate in a tin foil covered
bottle (light sensitive) and filter thru a 0.22 micron filter before use! You
would need 10-12 nm thick carbon films covered 300 mesh grids. Use a 10
microliter drop of your particles in ethanol, propyl or isoppropyl alcohol or
some solvent compatible with water ---- and put it on the carbon film for a
minute. Remove the excess alcohol from the edge of the grid with torn Whatman
#1 filter paper. Apply 5-10 microliters of stain for a minute and remove with
the filter paper method and repeat the procedure! Dry the grids from the edge
with torn Whatman #1 filter paper and then look at your grids in the TEM. You
will want to measure the white particles you see surrounded by dark stain.

George C. Ruben
Dept. Biological Sciences
Dartmouth College
Hanover, NH 03755
603-646-2144
603-646-1347 fax
George.C.Ruben-at-Dartmouth.EDU




From: wesaia-at-iastate.edu (Warren Straszheim)
Date: Wed, 08 Nov 1995 08:45:33 -0600
Subject: Re: Sample preparation

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} From: Bernard A Klazema
} Another problem we have is : we bought a new SEM (Philips XL30) and the image w
} e make must be printed on a Kodak 8600 PS dye sublimation printer. TIFF format.
} We like to have an overlay on the images with all the data like U marker and
} Mag on it, As far as I know this is only possible by a videoprinter, but is the
} re someone who can help me out with some software (Macro in windows?)
} We have in house Coral Paint and all the normal software packages from Micro-
} soft.

I have done well with the Recorder program that comes with Windows in the
Accessory
group. It provides macro capability for all Windows applications, not just
those with
built in macros. It can record mouse actions as well as keystrokes. I have
strung
several macros together with their own hot keys. Say the macro first selects
the
text tool, then a location, and perhaps even starts filling in the text. I
end that
macro there and define another one to finish the task. One might argue that
it is no
substitute for a good macro tool in the application itself, nor does it
allow editing
of the recorded macros, but it still can do a lot if one is careful and
creative.

I have also made use of the cut and past features of Windows apps when
labeling images
in PhotoStyler. Since most all text boxes will accept pasted text, I have
opened Notepad
or Write to compose a set of standard labels and then copied the appropriate
starter text
(to the Clipboard) for pasting into the image. I then customize it for the
particular
image or mag or exposure, etc. It has saved me a lot of time.
----------------------------------------------------
Warren E. Straszheim
270 MD Ames Lab/ISU, Ames IA, 50011
Phone: 515-294-8187 FAX: 515-294-3091
E-Mail: wes-at-ameslab.gov (or: wesaia-at-iastate.edu)

coal characterization and processing
electron microscopy, x-ray analysis, image analysis
computer applications





From: tothal-at-falcon.mufi.hu (Toth Attila)
Date: Wed, 8 Nov 1995 16:50:25 -0500
Subject: unsubscribe

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unsubscribe tothal-at-mufi.hu

Dr. Helmut SITTER
University Linz
A-4040 LINZ / Austria






From: spignole-at-ix.netcom.com (Susanne Brandom )
Date: Wed, 8 Nov 1995 07:43:28 -0800
Subject: Re: SEM Protocol for Insect Eyes

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} Anyone got a protocol for the preparation of the compound eyes of
insects
} (Drosophila) for scanning electron microscopy?
}
} If so, would you be willing to share it?
}
Sure.

We collect insects from the fluorescence light in our kitchen for
scanning electron microscopy examination. These are nicely dried
without collasping. The bugs (eyes and all) look great and several
have of the images obtained from these specimens have been used in
advertisement.

Susanne Pignolet Brandom
MicroWorld




From: spignole-at-ix.netcom.com (Susanne Brandom )
Date: Wed, 8 Nov 1995 07:30:39 -0800
Subject: Re: Microscopical demo-images site

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A list of images on the WWW is in the "Guide to Microscopy and
Microanalysis on the Internet" at the MicroWorld Resources and
News WWW site. The images are listed by subject. Each techniques
category also has sites with images marked.

MicroWorld Resources and News is at:
http://www.mwrn.com/

Please check the information at each site to determine if there are any
restritions on the use of the images. If you have any problems, please
send me a message.

Susanne Pignolet Brandom, Ph.D.
MicroWorld
708-548-6522




From: DDKJoe-at-aol.com
Date: Wed, 8 Nov 1995 13:29:01 -0500
Subject: Re: Sample prep - polymer nan...

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Owen,

In my prior life as a chemical engineer, I was responsible for a colloidal
silica unit. Whenever questions were raised about particle size
distribution, we would walk a sample over to our friendly electron
microscopist for analysis.

His secret was to perform a series of dilutions to very dilute levels of Si.
My recollection is that he did a series of three or four 1:100 dilutions
before drying a drop on a coated grid. This created a single particle thick
film of these normally sticky SiOx particles.

We were routinely dealing with discrete particle sizes from 6 to 150 nm.

Although it was 15+ years ago, I could dig up his name and number if you
would like to give him a call.

Good luck,
Joe Tabeling
Delaware Diamond Knives
800-222-5143




From: Chris Gilpin :      CGILPIN-at-fs1.sem.man.ac.uk
Date: Wed, 8 Nov 1995 16:36:17 BST
Subject: batch printing tiffs

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Fellow microscopists
We have a couple of EMs with digital imaging. We also have a lexmark 1200 dpi
laser printer. I need a software package which will allow me to print batches
of tiff files without loading the individual files first. It would also be
nice to be able to decide how many images to have per page.
I use ibmpc and mac on a novell 3.12 network.
Any help appreciated.


Chris



Chris Gilpin
Biological Sciences E.M. Unit
g452 Stopford Building
Manchester University
Oxford Road
Manchester
M13 9PT
U.K.
0161 2755170 phone
0161 2755171 fax




From: Strucural Biology Unit :      MICROSCOPY-at-sbsnov1.auckland.ac.nz
Date: Thu, 9 Nov 1995 09:15:58 GMT+1200
Subject: DIATOME address

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Could someone please send me the Fax number or preferably the
e-mail address for DIATOME in Switzerland?

Please send the message directly to me, not to the listserver.
Many thanks in advance.

Ken Goldie



Microscopy Unit
School Of Biological Sciences
The University Of Auckland
Level 1, Thomas Building
Private Bag 92019
Auckland, NEW ZEALAND

phone:(09) 373 7999 ext 5986
fax: (09) 373 7417




From: Strucural Biology Unit :      MICROSCOPY-at-sbsnov1.auckland.ac.nz
Date: Thu, 9 Nov 1995 09:15:58 GMT+1200
Subject: DIATOME address

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Could someone please send me the Fax number or preferably the
e-mail address for DIATOME in Switzerland?

Please send the message directly to me, not to the listserver.
Many thanks in advance.

Ken Goldie



Microscopy Unit
School Of Biological Sciences
The University Of Auckland
Level 1, Thomas Building
Private Bag 92019
Auckland, NEW ZEALAND

phone:(09) 373 7999 ext 5986
fax: (09) 373 7417




From: T. Page Owen Jr :      tpowe-at-conncoll.edu
Date: Wed, 8 Nov 1995 10:22:11 -0500 (EST)
Subject: digital SEMs

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We are finishing are shopping for a new SEM. Since I have not used a new
digital SEM before, I have some basic questions.

(1) Do we still need to purchase a Polaroid camera and high resolution
CRT system, or are people comfortable going completely digital? It looks
as if we omit the camera, we would have enough left over for a good
dye-sub printer and laser printer.

(2) Frame capture boards. Is a resolution of approx. 1K x 1K enough or
is the extra $$ for a 2K x 2K necessary?

We currently have a Zeiss TEM and are considering the new LEO SEMs. Any
comments on this new merger of Zeiss and Leica electron optics?

Thank you for your help. As we are a small college, our decision will
probably be around for a long time...

Page Owen
Connecticut College
Department of Botany
New London, CT 06320
(203) 439-2147

tpowe-at-conncoll.edu





From: BARBARA.HARTMAN-at-1773.220.SCHERING-PLOUGH.sprint.com
Date: Wed, 8 Nov 1995 12:14:15 -0500
Subject: Cataracts

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Has anyone heard of an increased incidence of cataracts in electron
microscopists ??? I have just been told that I have cataracts developing
and that it is unusual to see them in someone my age. I can't help but
wonder if my choice of profession had anything to do with it.

Thanks for any info.

Barbara Hartman
Schering-Plough Research Institute
Barbara.Hartman-at-Schering-Plough.sprint.com






From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Wed, 08 Nov 1995 17:19:18 -0600
Subject: Forier Transform Infrared Microspectroscopy

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Message-Id: {199511082221.QAA16596-at-bcm.tmc.edu}
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Dear Microscopists -

Does anyone use Forier Transform Infrared Microspectroscopy ("FTIR") on
biological materials? I would be interested in learning about this technique
and hearing of your experiences with it. This is a new method of identifying
compounds with microscopic resolution.


Joiner Cartwright, Jr., Ph.D.
Director, Electron Microscopy tel.: (713)798-4658
Department of Pathology, Rm.286-A FAX: (713)798-3945
Baylor College of Medicine Internet: joiner-at-bcm.tmc.edu
One Baylor Plaza Compuserve: 71555,1206
Houston, Texas 77030 U.S.A.






From: nano-at-ns1.LIHTI.ORG (Nanoprobes Inc.)
Date: Wed, 8 Nov 1995 10:46:31 -0500
Subject: Re: LM: colloidal gold enhancement

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The latest volume edited by M. A. Hayat, "Immunogold-Silver Staining:
Principles, Methods and Applications" (CRC Press, Boca Raton, FL, 1995) has
many articles on methods of silver enhancement. The earlier series,
"Colloidal Gold: Principles, Methods and Applications" (M. A. Hayat, Ed.;
Academic Press, San Diego, CA: Vols. 1, 2 (1989) and Vol. 3 (1991) have
some useful info as well.

Richard D. Powell
Nanoprobes, Inc (We sell silver enhancers too).
URL: http://www.lihti.org/research/ecodev/incubten/nano/home.html

} Do any of you have a current favorite method for enhancement (silver or
} whatever) of 5 to 10 nm colloidal gold for light microscopy? The person
} who is asking will be labelling plant goop on a slide, not sections of
} embedded material. And is darkfield the best way to view? I haven't
} had to look for gold with LM before - I rather presume silver
} enhancement would be the same as for TEM, but more...?







From: minter-at-kodak.com (John Minter)
Date: Wed, 8 Nov 1995 09:56:54 -0500
Subject: Re: Sample prep - polymer nanoparticles

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We do this type of analysis by cryoTEM of vitrified thin liquid films of
dispersed particles. We collect the images on a slow-scan CCD camera using
Gatan's DigitalMicrograph and do image analysis using NIH Image.
We compute the size distributions using a statistical package
called RS/1 (on the VAX or Sun) and fit the data to a lognormal
distribution with up to three modes.

We have tried aerosol methods and have not been satisfied with the
fraction of isolated particles. We do MUCH better with cryoTEM.
The good news is that one can automatically
select only isolated spheroidal particles from the images
containing some overlapping particles by calculating
the circularity of each feature (C=4*pi*area/perimeter**2). For such particles
we only keep features with 0.90 } C } 1.05. Verify this yourself by
measuring model images of agglomerated circles. By the way, it took me
almost a year to develop this capability to the point that I convinced
myself that I had done everything correctly. It was not a trivial
project.

} Hello;
}
} I'm working with a group that wishes to make morphological measurements on
} polymer nanoparticles using electron microscopy (SEM or TEM). The
} nanoparticles will likely be polyvinylpyridine, polycyanoacrylate, possibly
} polymethylmethacrylate. The nanoparticles should be in the range of
} 100-200nm, but they may well be bigger. They would like to see the whole
} range of particles and be able to report average particle diameter and the
} whole range of particle sizes (size polydispersity).
}
} Previous attempts, by placing drops of particles suspended a solvent on a
} SEM mount or TEM grid, have resulted in "clumps" of particles that are
} difficult to measure.
}
} Can anyone recommend a prodecure that might work for this situation. I have
} heard of (but am unfamiliar with) aerosol procedures, will that work?
}
} Also, can anyone recommend a few useful general references on electron
} microscopy of polymers. I have "Polymer Microscopy" by L. Sawyer & D. Grubb
} on order now.
}
} Thanks.
} Owen P. Mills
} Michigan Technological University
} Metallurgical & Materials Engineering
} Rm 512 MME Building
} Houghton, MI 49931
} 906-487-2002
} 906-487-2934 FAX
} opmills-at-mtu.edu






From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Wed, 08 Nov 1995 14:28:12 -0600
Subject: DragonDictate - Can anyone tell me about it?

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Does anyone out there in microscopy land use a software package called
"DragonDictate", and are willing to share their thoughts about it? We have
some people in our department who are considering such an automated
dictation setup. They are impressed with IBM's program, but are curious
about DragonDictate. Does anyone here in the Houston, Texas area have it and
want to show it off?

Joiner Cartwright, Jr., Ph.D.
Director, Electron Microscopy tel.: (713)798-4658
Department of Pathology, Rm.286-A FAX: (713)798-3945
Baylor College of Medicine Internet: joiner-at-bcm.tmc.edu
One Baylor Plaza Compuserve: 71555,1206
Houston, Texas 77030 U.S.A.






From: Mark Biedrzycki :      mtb-at-lithium.sem.Arizona.EDU
Date: Wed, 08 Nov 1995 10:03:01 -0700 (MST)
Subject: Re: SEM education

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On Tue, 7 Nov 1995, Bridgett Byrnes wrote:

} To MSA subscribers I am an undergraduate student seeking higher
} education in the fields of SEM and TEM. I will be graduating soon
} and I am searching for a college that will offer certification in EM.
} Can anyone help me in this area?
Come to the University of Arizona!
We have an outstanding Materials Science and Engineering
microscopy facility, and full semester courses on both
TEM and SEM.

Of course, there are many other fine public and private universities
offering EM classes. Gain access to a World Wide Web browser,
and look around. Try doing a web search for "microscopy and
education" or some such thing.

Best regards,

Mark T. Biedrzycki
Computer Networking Laboratory Materials Science and
for Microscopy Education (CNLME) Engineering Department
at the University of Arizona, Tucson





From: Jeffrey.Shield-at-mse.utah.edu (Jeff Shield)
Date: Wed, 8 Nov 1995 09:52:58 -0700
Subject: JEOL2000FX

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Greetings,

We are having a slight problem with our JEOL2000FXII. We have persistent
arcing illustrated by a flickering, unstable beam. We have cleaned the gun
area several times with little effect. The dark current is stable. We have
also tried several filaments. We also have adequate freon. So, we are at
the end of our rope.

I would be extremely grateful if anyone would have a suggestion on what to
look for to fix this thing. Cleaning procedures that you use would also be
helpful, since we hypothesize that is still the problem. Thanks for your
assistance. It could save us $$ in a service call.

Jeff
--------------------------------------------------------- U U
| Jeff Shield | U U
| Department of Materials Science and Engineering | U U U U
| University of Utah | U U U U
| Salt Lake City, UT 84112 | U U U U
| 801/581-3179 Fax: 801/581-4816 | UUUUU U
| | U U
--------------------------------------------------------- UUUUU
Of making many books there is no end, and much study wearies the body.
-Eccl 12:12





From: Peters-at-BSAC.UCHC.EDU (Klaus-Ruediger Peters)
Date: Wed, 8 Nov 1995 12:52:14 -0500
Subject: Re: Microscopical demo-images site

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Message-Id: {v01520d00acc698ef738d-at-[155.37.2.10]}
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Digital Micrograph-Demo Site is beeing organized by the MSA Education Committee.

The need for such a site is evident. Within the Education Committee of MSA
I try to organize such a "digital image data resource" site for all
microscopies. At this time, many laboratories offer plenty of images at
their web sites but a central directory and specific copyrights are
lacking. Everbody, who wants to contribute to a general "MSA Digital Image
Resource", please contact me. We will make the images available through the
MSA web server. Images may remain in local web resource directories and may
only be linked to the general register.

Thanks Klaus


******************************************************************************
* : *
* Klaus-Ruediger Peters, Ph.D. : Browse our WWW Home Pages: *
* Director, Molecular Imaging Laboratgory : *
* Biomolecular Structure Analysis Center : Molecular Imaging Laboratory *
* University of Connecticut Health Center : http://panda.uchc.edu/ *
* 263 Farmington Ave. : htklaus/index.html *
* Farmington, CT 06030-2017; U.S.A : Differential Hysteresis *
* : Processing Demo at http:// *
* Tel: (203) 679-3977; Fax: (203) 679-1989 : panda.uchc.edu./htbit/indiv/ *
* e-mail: Peters-at-BSAC.UCHC.EDU : /software_docs/dhp.html *
* : *
****************************************************************************
**






From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Wed, 08 Nov 1995 14:28:16 -0600
Subject: Computer virus scare

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Message-Id: {199511081930.NAA01089-at-bcm.tmc.edu}
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Microscopists -

Several months ago there was a posting on the Microscopy Listserver
concerning a super bad computer virus called the "Good Times" virus that
would end civilization as we know it. Almost immediately a number of replies
came back debunking it as rumor. Apparently the real virus was the panic and
paranoia that it spread. Well, the scare seems to have cropped up again here
in the Houston area.

My question is: Is it still considered bogus? Is there any further
development on this?


Joiner Cartwright, Jr., Ph.D.
Director, Electron Microscopy tel.: (713)798-4658
Department of Pathology, Rm.286-A FAX: (713)798-3945
Baylor College of Medicine Internet: joiner-at-bcm.tmc.edu
One Baylor Plaza Compuserve: 71555,1206
Houston, Texas 77030 U.S.A.






From: Gib Ahlstrand :      giba-at-puccini.crl.umn.edu
Date: Wed, 8 Nov 1995 10:43:26 -0600
Subject: Re: SEM Protocol for Insect Eyes

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In message {Pine.A32.3.91.951107204433.43418E-100000-at-san_marcos.csusm.edu} Kevin
Schram writes:
}
} Heh all!
}
} Anyone got a protocol for the preparation of the compound eyes of insects
} (Drosophila) for scanning electron microscopy?
}
} If so, would you be willing to share it?
}
} Contact me at:
}
} schra001-at-coyote.csusm.edu
}
}
} Thanks in advance!

Kevin,

I've done SEM of compound eyes of fruit flies (sorry, my Latin ain't good) using
my cold stage on my Philips 500SEM. The fly bodies were squished onto carbon
double stick tape on aluminum pin stubs, dabbed with a bit of carbon paint,
oriented so that the eyes were looking upward....(goodbye cruel world). Then
sample was placed onto liquid nitrogen pre-cooled cold stage for freezing,
quickly inserted into SEM and imaged at 1.5 kV. No metal coating was applied,
thus the reason for low kV and no charging is observed. If any frost was
initially observed, I'd shut off the beam, bleed a bit of dry nitrogen gas
through the chamber, pump vacuum down again and proceed.

Using this method, images were recorded up to about 2,000X of patterns of wild
and mutant fly eyes. I proudly call the technique LVLTLRLMLOSEM (low voltage low
temperture low resolution low magnification low overhead SEM). It also works
well for some delicate plant tissues.

If you do not have access to an SEM with cold stage you could try the ol'
fixation, dehydration & critical point drying method, but we tended to get
collapse of eye facets using that technique, but you might do well enough to get
the information you need.

Hope this helps. If you want to discuss further, contact me off line.


--

Gib Ahlstrand, MMS Newsletter Editor
Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
495 Borlaug Hall, St. Paul, MN 55108 (612)625-8249
612-625-9728 FAX, giba-at-puccini.crl.umn.edu

"MICROSCOPY & MICROANALYSIS 96" in Minneapolis, Minnesota, Aug.11-15, 1996





From: Michael Rock :      merock-at-u.washington.edu
Date: Wed, 8 Nov 1995 16:52:05 -0800 (PST)
Subject: Re: digital SEMs

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X-Sender: merock-at-homer10.u.washington.edu

Page- I would advise to spend the money on the 2k x 2k image capture (if
you can afford it), you'll be glad you did 2 years from now when 1k x 1k
is considered obsolete. And save your money on the Polaroid, it is
obsolete. Invest in two printers, a dye-sub for publication, and a 600-1200
dpi laser printer for working copies. Just my opinion. -Mike

On Wed, 8 Nov 1995, T. Page Owen Jr wrote:

} We are finishing are shopping for a new SEM. Since I have not used a new
} digital SEM before, I have some basic questions.
}
} (1) Do we still need to purchase a Polaroid camera and high resolution
} CRT system, or are people comfortable going completely digital? It looks
} as if we omit the camera, we would have enough left over for a good
} dye-sub printer and laser printer.
}
} (2) Frame capture boards. Is a resolution of approx. 1K x 1K enough or
} is the extra $$ for a 2K x 2K necessary?
}
} We currently have a Zeiss TEM and are considering the new LEO SEMs. Any
} comments on this new merger of Zeiss and Leica electron optics?
}
} Thank you for your help. As we are a small college, our decision will
} probably be around for a long time...
}
} Page Owen
} Connecticut College
} Department of Botany
} New London, CT 06320
} (203) 439-2147
}
} tpowe-at-conncoll.edu
}
}




From: pat_masarachia-at-Merck.Com (Pat Masarachia)
Date: Wed, 8 Nov 1995 16:11:54 EST
Subject: LR White immunolabeling-LM/EM

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Message-Id: {9511082215.AA29293-at-igw.merck.com}

There is some rule that when something is going well don't change
or stop anything.. for several months I had good success immuno-
gold labeling using a polyclonal antibody for an integrin antigen. I
could get specific labeling in .3 micron sections of bone embedded
in LR White (silver intensified gold) for LM or in thin LR White
sections for EM. The same specific labeling results occurred in a
dozen experiments; antigen absorption of the antibody and deletion
of antibody were negative controls. The antibody was specific and
dependable. I had to interrupt the project and did not return for
a year. Murphy's law takes over. With the same antibody, using
aliquots stored at -20 C as per manufacturers instructions as well
as aliquots stored at -70 C, I cannot get labeling on sections
cut from the original blocks. The antibody still works on pre-
embedded tissue culture. On top of this, the original manufacturer
went out of business. The antibody produced by a new company with
the original protocol does not work. I thought that maybe the LR
White blocks after a year further polymerized to reduce antigenicity.
I worked up newly embedded tissue, freshly cut sections, and still
got no label. I've tried different secondary antibodies and different
blocking and different buffers -- no label. A different antibody for a
different antigen does specifically label in sections from old
blocks or new. So... can an antibody 'die' even if stored properly?
Sorry for this long winded tale but with no substitute available
commercially for this particular antibody I'd love to know what
happened.
Thanks for any comments.

Pat Masarachia
Bone Biology
Merck Research Laboratories
West Point, PA 19486






From: William Tivol :      tivol-at-wadsworth.org
Date: Wed, 8 Nov 1995 17:39:02 -0500 (EST)
Subject: Re: High Resolution Microanalysis

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} I am working on an undergraduate thesis to improve the resolution of xray
} detectors for EDS systems, likely using a cryogenic detector. Our
} goal is to achieve a resolution of about 10eV (for Mn K alpha), while
} retaining reasonable count rates (allowing faster processing then
} wavelength dispersive spectrometry).

Dear Don,
The theoretical limitation to energy resolution from a solid-state
detector, like those used in EDS, comes from counting statistics. For an
x-ray of ~6 keV to have a resolution of 10 eV, you need to produce 60 elec-
tron-hole pairs per eV of energy loss. This gives 360,000 e-h pairs per
6-keV-x-ray, or +-600 counts per measurement. N.b., this assumes each x-ray
is fully absorbed, no small background counts occur simultaneously, and none
of the other problems with getting 6 keV deposited in the detector for each
x-ray occurs. Unless you are going to operate on a different principle from
that used in solid-state detectors, your first problem is to find a material
with a small enough band gap. Maybe an appropriate metal will serve as a
semiconductor at a low enough temperature (} 1 K). Good luck; this seems a
very complicated and difficult problem for a thesis--especially an undergrad-
uate one.
Yours,
Bill Tivol





From: James C. Long :      jlong-at-bga.com
Date: Wed, 08 Nov 1995 11:03:34 -0600
Subject: Voluteers Needed - Science by Mail Program

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Message-Id: {199511081626.KAA26696-at-zoom.bga.com}
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Greetings,

I wanted to make folks aware of a program for getting kids into science. I
have participated in this progam for several years and find it very
rewarding. The kids send letters and questions that are fun to read, and
most seem very interested in science. If you have just a few hours to
spare, please consider becoming a voluteer scientist. (details below)

Thanks,

James

(Forwarded from sci.chem)
SCIENCE-BY-MAIL PROGRAM SEEKING VOLUNTEER SCIENTISTS

Science-By-Mail is a pen-pal program from the Museum of Science in Boston,
Massachusetts that teams scientists with children in grades 4 through 9.
Due to an increase in membership this year we need additional scientists
for the 1995-96 program year. All of the scientists in our program act as
pen-pal mentors and correspond with up to five groups of 1-4 children or
with one classroom of seven groups of 1-4 children. We mail out two
activity packets per year (once in December and once in March) for you to
review, and then correspond with your pen-pals about the activities in the
packets.

The commitment requires approximately 20 hours per program year (November
through June). Our topics this year are the Science of Sports and
Planetary Science. Volunteers do not need to be experts in the fields of
the topics, we provide information on each topic to the scientists, for
each activity.

In order to be a Volunteer Scientist you need to have a bachelors degree
in a science or technology related field. You also need to have the
desire to inspire scientific curiosity in children.

If you are interested in volunteering for Science-By-Mail please call
1-800-729 3300 or 617-589-0437 and ask for Melissa Cotter or Monica
Parker. If you get our voice mail just leave your name, phone number and
fax number and we will send you information right away. Thank you in
advance for your help!
==============================================================================
James C. Long
Manager/Materials Analysis Lab
Electrosource, Inc.
512-445-6606
jlong-at-bga.com





From: mager-at-unixg.ubc.ca (Mary Mager)
Date: Wed, 8 Nov 1995 20:55:43 -0800
Subject: Re: Digital SEMs

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Dear Page:
Despite what you have heard, all the SEMs have been digital for at least 10
years, now, at least where it is practical to be digital. They all still go
back to analog for the Polaroid, since that is the most practical way to
get photographic resolution. Remember that you can only record the
resolution that the instrument puts out and recording a 2K by 2K image out
of a 800 by 600 digital output will only get you the lower number
resolution. By my experience with SEMs and digital imaging systems, you
still need the photographic output for the best quality images and the
convenience of film and negative. Most SEMs can output at least 2500 lines
on the photo CRT. I have yet to see a laser printer or dye-sub printer
which truly gives photo quality images when examined closely. The printers
are great for "quick and dirty" and save the cost of the Polaroid film when
it is not needed, so it is nice to have both, but if the SEM is just a big
camera, then you should judge it by the quality of its best output. I would
be very reluctant to lose the photographs.
Best of luck.
Mary

Mary Mager
Electron Microscopist
Metals and Materials Eng, UBC
309 - 6350 Stores Rd.
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648, fax: 604-822-3619
email: mager-at-unixg.ubc.ca






From: shaun.sandow-at-anu.edu.au (Shaun Sandow)
Date: Thu, 09 Nov 1995 16:00:56 +1000
Subject: slot grid problems

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Dear Microscopists,

I'm looking at serial sections of nerves on formvar (1% in chloroform)
coated slot copper grids (pretty standard stuff). The grids are acetone and
distilled water cleaned several times and coated as per standard (which has
worked consistently well for 4 years). For the past few months I've been
having considerable trouble with what appeared to be focus problems. It now
appears that the film is "shifting". The film is carbon stabilised and
clean; we've tried modifying formvar coating preparations, carbon coating
and specimen storage, but the film movement is still present. It appears
"tight" when on the grid, but appears to shift under the beam (as indicated
by what appeared to be focussing problems). Anyone with a
suggestion????????????????????????/

Thanks,
______________________________________________

Shaun Sandow,
Division of Neuroscience,
John Curtin School of Medical Research,
Australian National University,
ACT 0200

Ph. (06) 249 4782
Fax. (06) 249 2687





From: John Millar :      JJMILL-at-bunyip.ph.rmit.edu.au
Date: Thu, 9 Nov 1995 16:45:17 EST-10
Subject: Re: Computer virus scare

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} Several months ago there was a posting on the Microscopy Listserver
} concerning a super bad computer virus called the "Good Times" virus that
} would end civilization as we know it. Almost immediately a number of replies
} came back debunking it as rumor. Apparently the real virus was the panic and
} paranoia that it spread. Well, the scare seems to have cropped up again here
} in the Houston area.
}
} My question is: Is it still considered bogus? Is there any further
} development on this?


Info from our virus experts is that it is not possible to spread by
email unless there is an attached file which is actually run. Anyone
doing this would be asking for trouble. The conclusion is that Good
Times is not for real, but since we have had recent severe problems,
I am interested in information.
The problems have been with MOnkey and MonkeyB viruses which are
very tricky.
cheers
jjm
Professor John J. Millar
Department of Applied Physics and
Electron Microscope Unit
RMIT, GPO Box 2476V, MELBOURNE AUSTRALIA 3001
+613 9660 2602 fax 613 9660 5290
email jjmill-at-rmit.edu.au




From: m.blackford-at-ansto.gov.au (Mark Blackford)
Date: Thu, 9 Nov 1995 15:58:10 +1100
Subject: Re: JEOL2000FX

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Message-Id: {199511090458.PAA04890-at-atom.ansto.gov.au}
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} Greetings,
}
} We are having a slight problem with our JEOL2000FXII. We have persistent
} arcing illustrated by a flickering, unstable beam. We have cleaned the gun
} area several times with little effect. The dark current is stable. We have
} also tried several filaments. We also have adequate freon. So, we are at
} the end of our rope.
}
} I would be extremely grateful if anyone would have a suggestion on what to
} look for to fix this thing. Cleaning procedures that you use would also be
} helpful, since we hypothesize that is still the problem. Thanks for your
} assistance. It could save us $$ in a service call.
}
} Jeff
} --------------------------------------------------------- U U
} | Jeff Shield | U U
} | Department of Materials Science and Engineering | U U U U
} | University of Utah | U U U U
} | Salt Lake City, UT 84112 | U U U U
} | 801/581-3179 Fax: 801/581-4816 | UUUUU U
} | | U U
} --------------------------------------------------------- UUUUU
} Of making many books there is no end, and much study wearies the body.
} -Eccl 12:12

Jeff,

your microscope is not the only 2000FX or FXII to suffer this problem. We
have one of each in our labs, both suffered this flicker in illumination
and I know of at least 3 others in Australia. In our case the cermic
insulators in the gun chamber had suffered numerous dicharges which left
tracks. These tracks facilitated further discharges and the whole problem
snowballed. We tried cleaning the insulator from the 2000FX but with no
success. In July 1993 we installed a new insulator (at great expense).
When the 2000FXII developed the same problem in October 1994 we opted to
convert to SF6 (from freon) as the HT tank and gun insulation gas. This
involved replacing the entire gun assembly and overhauling the HT tank to
make it compatible with SF6. This was VERY expensive.

We have had no repeat of the flicker problem so far. We intend performing
an overhaul of each gun assembly bi-annually in the hope that the
discharges can be avoided in future.

Mark Blackford
TEM Group
Materials Division, Ansto
PMB 1,
Menai, N.S.W.
Australia
2234






From: GVKM07A-at-PRODIGY.COM (DR CHARLES A GARBER)
Date: Thu, 09 Nov 1995 00:04:54 EST
Subject: TEM Sample Preparation

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-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --

On November 7, Bernard A. Klazema asked about the thin sectioning of
"glass filled materials". He suggested this would result in the
"ruining" of a diamond knife. His samples are impact modified glass
fiber reinforced plastics.

Well, he is half right and half wrong. With practice one can very
definitely do ultramicrotomy on such samples without "ruining" the
diamond knife. However, one has to appreciate the following:

a) The "wear and tear" put on the diamond knife is considerable, with
more "wear and tear" put on by the inexperienced ultramicrotomist, and
less, by the experienced ultramicrotomist. But no matter what is the
experience, the point is that this kind of microtomy is going to put
"wear" on the knife at a rate far greater than that what would be
encountered for straight soft tissue samples.

The depreciation of the diamond knife is a significant cost factor in
the conduct of this type of work. In our own laboratory, we can
usually get 15-50 samples of this type out of each knife, depending on
the percentage loading of the glass fibers, and yes, the experience of
the person doing the work. But it can be done, provided one is willing
to pay the cost associated with the knife's depreciation.

b) We recommend a "materials science" diamond knife (of the type
offered by our firm because we do not know of anyone else offering such
a product), one that is basically the same "angle" as a life science
diamond knife. We do not like the idea of using a "blunter" angle, to
get longer knife life because the possibilities of compression effects
increase greatly and also therefore various artifacts from the cutting.
We have found that the distortion that results, even when done cryo, of
these kinds of samples, with a blunter angle knife, caused round rubber
modifier particles to come out looking like elipses, oriented in the
direction of the knife.

c) The materials science knives supplied by our firm are not
"striation free", they do contain, at the edge a small number (e.g. not
more than a few) of fine striations. Such knives would not be
acceptable for typical life science work. But this small population of
fine striations that is there to begin with is smaller than the much
larger number of striations put into the knife edge after taking the
first slice on a glass fiber filled plastic.

So my point is why pay more for the "perfect" knife when one with a few
striations to begin with will do just as well? And at the same time, a
substantial amount of money can be saved relative to the price paid for
the typical "life science" knife (sans striations).

d) For visualizing the impact modifiers, osmium tetroxide or in the
case of acrylic modified sytems, ruthenium tetroxide, can be used to
demonstrate the structure and morphology of such systems, including the
spatial arrangement of the glass fibers.


Hope these comments will be useful.

Chuck

======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail:
GVKM07A-at-prodigy.com
West Chester, PA 19381-0656 USA Customer Service:
SpiSupp-at-aol.com

########################################################
WWW: http://mail.cccbi.chester.pa.us/spi/spihome.html
########################################################
======================================================





From: David Dryden :      djd-at-physics.unimelb.EDU.AU
Date: Thu, 9 Nov 1995 16:23:26 +1100
Subject: Re: JEOL 2000FX

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Jeff,
You are most probably correct, that is, the gun chamber is
contaminated. Find out at what accelerating voltage the beam
becomes stable, if it is a little less than 200kV the gun chamber
is probably the problem. Also if you monitor the gun chamber
vacuum any HT instabilities will coincide with variations in
the gun vacuum. This will give you an idea whether the HT
problem is in the gun or the HT tank. Also you can isolate the
HT tank from the gun and bring up the HT to 200kV. By
monitoring the CH output from the HT tank with a CRO you should
be able to monitor the high voltage ripple waveform. This
waveform is the ripple of the HT at whatever kV the HT is
applied at and if any instabilities are present they will be
seen as sharp rapid spikes within this waveform. The typical
waveform pattern is {5mVp-p AC -at- 20ms/cm. Be carefull not to
blow up your CRO. If any large discharges occur in the HT it
will kill your input pre-amps to the CRO.
You say the beam current is stable but the flickering is
evident. The beam current is definitely unstable it is the
meter that cannot detect any small rapid variations.
Have you tried conditioning the HT above 200kV for a
short period of time and see whether the beam is stable after
this.
I still rekon the problem is with contamination. Especially
since you say you have cleaned the gun chamber several times.
Every time you have cleaned this region no matter how careful
you are you will have left behind some sort of contamination.
I never use any polish except for the wenelt assembly and anode
only when definite contamination is present. All other parts
are wiped clean with a lint free cloth soaked in liquid freon.
All polished parts must be ultrasonically cleaned in freon
and then baked before re-assembly.
Even with this procedure the beam instabilities you
are experiencing I to have seen at 400kV though.
The routine maintenance procedure to eliminate this
slight gun chamber beam instability is to create a HT glow
discharge in the gun chamber. This is done by raising the
gun chamber vacuum to 210uA on the pirani gauge and applying
the HT at (I do not know for the 200kV machines) 150kV for the
4000EX series machines. You have to overide a lot of protection
circuits to be able to achieve this and experience and
care must be taken into account. If you need help I have a
complete discription explaining this procedure but first check
with Jeol to see if it can be done for your machine and at
what kV is best. The procedure is done with an inert gas,
usually Nitrogen, and what happens is a glow discharge which
moves all the contamination around. About 10% of the
contamination is removed by the rotary pump and the rest is
moved out the way of the general beam line. Within time
the procedure is needed again. For the 400kV machines this
is routine about every 6 months.
In summary I can guess your machine was O.K. untill
you opened the gun for some other reason. And once closed and
maybe some time down the track this instability happened.
I have several questions that will help to pin point
the problem and maybe it is best you e-mail me direct.
What is the gun vacuum?
What kV does the beam become stable?
Have you tried HT conditioning?
Have you cleaned the upper and lower anodes?
Have you isolated the probelm to the Gun region by
monitoring the Gun chamber vacuum?

Regards,
David Dryden
School of Physics
University of Melbourne
Parkville, VIC. Australia, 3052.
djd-at-electron.ph.unimelb.edu.au
http://www.ph.unimelb.edu.au/~djd/diff-home.html





From: Stephen Edgar :      s.edgar-at-auckland.ac.nz
Date: Thu, 9 Nov 1995 17:44:43 NZS
Subject: Re: Keeping absolute ethanol dry - a summary

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Hi everyone,

This is a summary of the responses to my question of two weeks ago,
which was: "How do I keep commercial absolute ethanol dry once the
container has been opened?"

Firstly, to those who replied my thanks again, even though you should
each have had a direct response from me by now.


Desiccant "packaging":
Almost everyone suggested that I put my molecular sieve inside
dialysis tubing to keep the dust out of the ethanol. Well, I've tried
that in the past but the dialysis tubing we get here is squashed flat
and *very* hard to open into a tube in its dry state. However I've
now been told to tie off one end of the flat tubing then blow
compressed air or similar into the open end. Thanks for that one,
Joan.


What desiccant to use:
Mostly respondents recommended molecular sieve, either alone or with
silica gel as an indicator. There is also at least one proprietary
brand of mol. sieve with a built-in indicator (probably not readily
available in New Zealand).

Mel suggested dried CuSO4 which is self-indicating, turning from
white to blue when it absorbs water.

One suggestion was to put magnesium metal in the ethanol to react
with the water. I wasn't sure about that one - after reading the
Merck Index I thought that if enough water was present the result
*could* be a solution of Mg hydroxide with an alkaline pH.


Alternatives to solid desiccants:
Bo and Tobias mentioned acidified DMP (2,2-dimethoxypropane), either
as an additive to the ethanol or as a stand-alone dehydrating agent.
Apparently acid.-DMP reacts with water to form acetone and methanol.
Thanks guys, it is probably good as an additive but I want to keep
things cheap and easy! The little I've read about DMP hasn't
recommended it as a dehydrating agent on its own (extracts lipids, I
think), and again there is the problem of increased expense.

Mark suggested fitting a plunger dispenser to the bottle of ethanol
as soon as it is opened, combined with molecular sieve. Stewart
suggested something similar but with calcium chloride in the air
intake instead of mol. sieve in the ethanol.



What I have decided to do:
Well, pretty much nothing actually. For reasons of economy and
convenience I preferred the suggestions of either mol. sieve or dry
CuSO4 in dialysis tubing, but I then asked a professor of chemistry
which he would choose and he wasn't enthusiastic about either of
them! He thought molecular sieve *might* change the pH "though it
shouldn't" and that CuSO4 "probably isn't very efficient".

So whenever I open a new winchester (2.5 litres) of ethanol I will
simply decant it into smaller (500ml/1 pint) oven-dried bottles, and
then open them one at a time to top up the 125ml bottle that is part
of the dilution series in the fume hood. For others in the dilution
series (up to 95%) we will continue to use commercial "drum" ethanol.

So that was a surprise ending to this story, wasn't it?


Postscript:
It turns out that this is exactly what The Southernmost EM Unit In
The World (in Dunedin, New Zealand) has been doing with their ethanol
for some time. Thanks for your message Allan, and I love the new
signature file.... is that a yellow-eyed penguin? - I only have a
mono screen :-) .


Regards

Stephen Edgar

Electron Microscope Unit, Pathology Department
School of Medicine
University of Auckland
Private Bag 92019
Auckland
New Zealand

email address: s.edgar-at-auckland.ac.nz
Phone : +64-9-3737599 extn 6473 (GMT + 12h)
Fax : +64-9-3737459




From: Henrik Kaker, SZ - Metal Ravne :      Henrik.Kaker-at-guest.arnes.si
Date: Thu, 09 Nov 1995 11:04:29 +0000 (GMT)
Subject: New WWW

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Dear All,

In our SEM-EDS laboratory we setup a new WWW:

URL=http://www2.arnes.si/guest/sgszmera1/index.html


Henrik Kaker
E-mail: Henrik.Kaker-at-guest.arnes.si





From: casix-at-public.sta.net.cn (CASIX)
Date: Thu, 9 Nov 1995 14:58:08 +0800
Subject: Need Help on Parameters of OPO and minilaser?

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Dear Friends,

A friend told me many people working on Spectroscopy and Microscopy
are interested in mini-laser (cw, 532 nm and 1064 nm) and optical
parametric oscillator (OPO, pulsed, tunable from 210 nm to 2500 nm).
Now, we are making mini-laser, OPO and optics for laser users. Can
anyone tell me the parameters of OPO and mini-lasers needed in
Spectroscopy and Microscopy?

Thank you in advance for your help.

Jiwu Ling
CASIX, Inc., PO Box 1103, Fuzhou, Fujian 350014, China
Fax: 86-591-366-6957
E-mail: casix-at-public.sta.net.cn
casixus-at-aol.com






From: Helen.Hassander-at-polymer.lth.se (Helen Hassander)
Date: Thu, 9 Nov 1995 08:50:45 +0200
Subject: Re:Sample prep - polymer nanoparticles

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Answer, Sample prep - polymer nanoparticles
Latex particles have a very low contrast in TEM. They may also be
filmforming. Therefore the best way to look at them is to use negative
staining. The latex should be diluted until it is almost clear. A water
solution of Uranyl Acetate (0.5%) is added to the latex. Place a small drop
on a formvar coated grid (400 mesh). When you look in the TEM you should
see dark rings around the particles. You may have to try a few times until
you obtain the correct dilution.
Good Luck!
Helen Hassander






From: ENERGY BEAM SCIENCES, INC :      75767.640-at-CompuServe.COM
Date: 09 Nov 95 08:18:39 EST
Subject: re:nitride and boride standards

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On Nov. 7, Alfred Kracher asked for sources of light-element microprobe
standards, like nitrides and borides of heavy metals. I have come up with the
following:
Boron Nitride
Lanthanum Hexaboride
Titanium Nitride
Silicon Nitride
Boron Trioxide
Boron Carbide
Please e-mail me directly for boron and nitride percentages in these compounds.
Steven Slap, Vice-President
Energy Beam Sciences





From: Helen.Hassander-at-polymer.lth.se (Helen Hassander)
Date: Thu, 9 Nov 1995 16:01:28 +0200
Subject: Re:Sample prep - polymer nanoparticles

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Answer, Sample prep - polymer nanoparticles
Latex particles have a very low contrast in TEM. They may also be
filmforming. Therefore the best way to look at them is to use negative
staining. The latex should be diluted until it is almost clear. A water
solution of Uranyl Acetate (0.5%) is added to the latex. Place a small drop
on a formvar coated grid (400 mesh). When you look in the TEM you should
see dark rings around the particles. You may have to try a few times until
you obtain the correct dilution.
Good Luck!
Helen Hassander






From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Thu, 09 Nov 1995 11:02:17 -0600
Subject: Re: Computer virus scare

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Message-Id: {199511091604.KAA14804-at-bcm.tmc.edu}
X-Sender: joiner-at-bcm.tmc.edu
X-Mailer: Windows Eudora Version 2.1.1
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

John -

It seems that it takes a lot of water to put out fires like this. Doug
Cromey suggested the following www sites as reference:

http://www.informatik.uni-trier.de/~bern/GoodTimes-Hoax/
http://cbrc-a12.mgh.harvard.edu/docs/GoodTimesHoax.html
http://www.nsm.smcm.edu/News/GTHoax.html
http://www-mcb.ucdavis.edu/info/virus.html

Joiner
***********************

At 04:45 PM 11/9/95 EST-10, you wrote:
}
} ....Info from our virus experts is that it is not possible to spread by
} email unless there is an attached file which is actually run.....





From: Bridgett Byrnes on Wed, Nov 8, 1995 2:45 AM
Date: 9 Nov 1995 09:03:00 -0800
Subject: SEM education

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Message-ID: {n1396214643.65845-at-ms.sjdccd.cc.ca.us}

Dear Bridgett


San Joaquin Delta College in Stockton, CA offers a 2 yr program certification
in microscopy. We will be adding training in focused ion beam next semester
as well. We have done so since 1970 and have graduates all over the United
States. If you send me your address, I will send info.
Sincerely,
Judy Murphy

Judy Murphy, PhD
Dept. of Microscopy
San Joaquin Delta College
5151 Pacific Ave
Stockton, CA 95207

Phone: 209/474-5284
FAX: 209/474-5649
e-mail: murphy.ms.sjdccd.cc.ca.us

________________________________________________________

To MSA subscribers I am an undergraduate student seeking higher
education in the fields of SEM and TEM. I will be graduating soon
and I am searching for a college that will offer certification in EM.
Can anyone help me in this area?







From: modum-at-gatan.com (Michael Odum)
Date: Thu, 9 Nov 1995 09:14:32 -0700
Subject: Re: SEM education

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Message-Id: {199511091710.JAA08372-at-core.gatan.com}

On 11/7/95 Bridgett Byrnes sent:

} To MSA subscribers I am an undergraduate student seeking higher
} education in the fields of SEM and TEM. I will be graduating soon
} and I am searching for a college that will offer certification in EM.
} Can anyone help me in this area?
}

Everyone knows that the only place to go for EM training is San Joaquin
Delta College in Stockton, CA. They have a fully accredited two year course
for SEM and TEM of biological, or crystalline, materials microscopy. The
address for the school is:
San Joaquin Delta College
Electron Microscopy Lab Holt 121
5151 Pacific Ave.
Stockton, CA. 95207
Tel: (209) 474-5246

The head of the crystalline materials program is Dr. Frank Villalovoz
and the head of the biological materials program is Dr. Judy Murphy. If you
need more information ask around because we graduates of the program are
everywhere.

Good Luck.

Michael W. Odum
Spec. Prep. Tech.
Gatan, Inc.
6678 Owens Dr.
Pleasanton, CA. 94588
Tel: 510-463-0200
Fax: 510-463-0204
E-Mail: modum -at-gatan.com






From: Murphy, Judy :      murphy-at-ms.sjdccd.cc.ca.us
Date: 9 Nov 1995 10:15:17 -0800
Subject: Film/Paper Processors

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Message-ID: {n1396210186.30918-at-ms.sjdccd.cc.ca.us}

We are having to get rid of our darkroom to make way for another instrument
THUS we need to replace the functions of the darkroom. I would appreciate
information about processors that can do both paper (RC papers)and film (EM
film 4489, SO163, Kodak 4127 and Ektapan). I am familiar with the Mohr
processor but need to know if there are any other good ones out there.
Thank You in advance for the info,
Judy Murphy


Judy Murphy, PhD
Dept. of Microscopy
San Joaquin Delta College
5151 Pacific Ave
Stockton, CA 95207

Phone: 209/474-5284
FAX: 209/474-5649
e-mail: murphy.ms.sjdccd.cc.ca.us





From: George.C.Ruben-at-Dartmouth.EDU (George C. Ruben)
Date: 09 Nov 95 07:53:43 EST
Subject: Re: slot grid problems

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Message-id: {6675456-at-prancer.Dartmouth.EDU}

With a problem of this kind you can not narrowly focus your TEM beam but you
must spread it out over a very large area--- This way you reduce local thermal
gradients nearyour intended picture location! Plastics films are always
difficult because they heat and expand in the electron beam and change their
height and focus!

George C. Ruben
Dept Biological Sciences
Datmouth College
Hanover, NH 03755
tel 603 646-2144
fax 603 646-1347
----------------------------------------
Dear Microscopists,

I'm looking at serial sections of nerves on formvar (1% in chloroform)
coated slot copper grids (pretty standard stuff). The grids are acetone and
distilled water cleaned several times and coated as per standard (which has
worked consistently well for 4 years). For the past few months I've been
having considerable trouble with what appeared to be focus problems. It now
appears that the film is "shifting". The film is carbon stabilised and
clean; we've tried modifying formvar coating preparations, carbon coating
and specimen storage, but the film movement is still present. It appears
"tight" when on the grid, but appears to shift under the beam (as indicated
by what appeared to be focussing problems). Anyone with a
suggestion????????????????????????/

Thanks,
_________________________________




From: gwe-at-biotech.ufl.edu (Greg Erdos)
Date: Thu, 9 Nov 1995 08:39:08 GMT
Subject: Re: slot grid problems

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} Dear Microscopists,
}
} I'm looking at serial sections of nerves on formvar (1% in chloroform)
} coated slot copper grids (pretty standard stuff). The grids are acetone and
} distilled water cleaned several times and coated as per standard (which has
} worked consistently well for 4 years). For the past few months I've been
} having considerable trouble with what appeared to be focus problems. It now
} appears that the film is "shifting". The film is carbon stabilised and
} clean; we've tried modifying formvar coating preparations, carbon coating
} and specimen storage, but the film movement is still present. It appears
} "tight" when on the grid, but appears to shift under the beam (as indicated
} by what appeared to be focussing problems). Anyone with a
} suggestion????????????????????????/
}
} Thanks,
} ______________________________________________
}
} Shaun Sandow,
} Division of Neuroscience,
} John Curtin School of Medical Research,
} Australian National University,
} ACT 0200
}
} Ph. (06) 249 4782
} Fax. (06) 249 2687
}
} } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } }
You might try "Grid Glue"

The recipe can be found under "Tips & Tricks" at
http://www.biotech.ufl.edu/~emcl

Just click the Wizard
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Greg Erdos Phone: 904-392-1295
Scientific Director,
ICBR Electron Microscopy Core Lab
218 Carr Hall Fax 904-846-0251
University of Florida E-mail: gwe-at-biotech.ufl.edu
Gainesville, FL 32611
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-





From: Richard E. Edelmann :      EDELMARE-at-CASMAIL.MUOHIO.EDU
Date: Thu, 9 Nov 1995 13:19:45 -500
Subject: Re: Digital SEMs

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Skipping the HR-Polaroid output.

I would have to agree with Mary, and say don't give up the Polaroid
yet, but with the following considerations (Which, since I'm not
intimately familar with the LEO SEM, you should look at):

(1) SEM's with direct analog output to the HR-photo screens, and the

polaroid NEGITIVES are generally capable of 2500 lines of resolution,

at typical SEM photo-rates. 2k x 2k is still alittle less than that
but you generally never use all 2500 lines on a neg. anyway.

(2) generally printers (1200dpi laser, or 300/600dpi
dye-sublimation) can not ultimately give the same resolution as the
polaroids.

(3) On some digital SEM's the output to the HR-photo screen is not
the original analog signal, but rather the digitally captured image
at its resolution (1k x 1k or 2k x 2k, more?). Therefore, if you
send a 1k x 1k digital image to a polaroid HR-photo CRT capable of
2500 lines, you will not be getting the full resolution of the
polaroid anyway so you might want to leave off the polaroid.

(4) Polaroid negs are very useful for a variety of photographic
imaging needs, which can not be met with affordable digital output
devices. It would be very useful to not only output current specimen
images (i.e. specimens in the scope) but also stored images from disk
to the HR-photo CRT (even images not generated on the scope? or
after manipulation?) as if the Photo CRT is another digital output
device like a printer.
For this a 35mm HR digital output device might serve equally as
well, and more usefully for other applications (i.e. slides for
presentations?), however as was just discussed on the list a few
weeks ago these can be very pricey ($6-10k), but might be an option
instead of the polaroid [35mm + good grey scale + dye sub printer =
polaroid output ? $ ?]






From: Paul Webster :      Paul.Webster-at-quickmail.yale.edu
Date: 9 Nov 1995 16:06:35 -0400
Subject: Re: LR White Immunolabeling

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Message-Id: {n1396189115.76882-at-QuickMail.Yale.edu}

Pat Masarachia writes about problems when labeling sections through LR White
embeded block of tissues and cells. She assumes that either the primary
antibodies, which seem to have been properly stored, or the further
polymerization of the block are at fault. However, there is no description of
the visualization probe that is being used. If the probe is a gold-conjugated
immunoglobulin then this may be the explanation for there being no EM labeling.
This is because these gold probes are very unstable and the protein will
separate from the gold over time (this starts even before purchase). One result
of this is that the labeling efficiency (or sensitivity) of the probe is
gradually reduced over time. It is possible also for the whole probe to just
stop working. If the labeling protocol has stopped working for EM and LM I
would suspect the latter.

Personally, I think that the best strategy for routine immunolabeling is to buy
only protein A-gold (5 and 10 nm particle sizes) and use only these two probes
for all labeling protocols. Only by doing this can the probes be constantly
monitored for activity. If immunoglobulin-gold is used, firstly a service lab
has to buy enough probes to cover all potential experiments (anti-rabbit,
anti-mouse, anti-rat, anti-guinea pig, 5 nm and 10 nm particle size) and
secondly, each probe must be tested for activity if it has been stored for long
periods.

I just threw that last part in to add a little controversy and perhaps start a
debate about the pros and cons of different gold probes and perhaps what are the
best storage conditions for primary antibodies and colloidal gold probes. I
know there are exceptions to using protein A-gold and some advantages to using
gold-conjugated antibodies but most of us seem to be placed in a role where we
have to help others at least some of the time. Under these conditions simple is
best.

Sorry Pat for the long reply. Feel free to contact me if you still have
questions.

Paul Webster
Center for Cell Imaging
Yale School of Medicine
http://info.med.yale.edu/cellimg
Tel: (203) 785 3219






From: South Bay Technology :      73531.1344-at-CompuServe.COM
Date: 09 Nov 95 18:40:24 EST
Subject: Metallographic Presses

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We are looking for a used mounting press for making metallographic samples from
bakelite. We won't be running a large volume so a manual press is fine. As
long as it is working we'll consider it. Please let me know if anyone has one
that they'd like us to take off their hands!

Thanks!

David Henriks TEL: 800-728-2233 (toll-free in USA)
South Bay Technology, Inc. 714-492-2600
1120 Via Callejon FAX: 714-492-1499
San Clemente, CA 92673 USA e-mail: 73531.1344-at-compuserve.com





From: William Tivol :      tivol-at-wadsworth.org
Date: Thu, 9 Nov 1995 17:49:33 -0500 (EST)
Subject: Re: slot grid problems

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Dear Shaun,

} ... For the past few months I've been
} having considerable trouble with what appeared to be focus problems. It now
} appears that the film is "shifting". The film is carbon stabilised and
} clean; we've tried modifying formvar coating preparations, carbon coating
} and specimen storage, but the film movement is still present. It appears
} "tight" when on the grid, but appears to shift under the beam (as indicated
} by what appeared to be focussing problems). Anyone with a
} suggestion????????????????????????/
}
I have had good luck with specimens which shift under normal beam
conditions by using very low beam currents (~10 pA). In order for this to
give you a decent image, you need either to have a long exposure time or to
use very sensitive film. LoDose or MRF32 will be sensitive enough, but they
have large grain--there is still no free lunch. If you have a very sensitive
video system, that will make focusing easier--we have an intensified CCD which
gives a useful image at video rates, so focusing can be done in a few seconds.
I suppose that using even a large mesh grid would not be satisfactory, but if
it is, then that may also stabilize your specimen. Good luck.
Yours,
Bill Tivol




From: modum-at-gatan.com (Michael Odum)
Date: Thu, 9 Nov 1995 09:14:32 -0700
Subject: Re: SEM education

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Message-ID: {n1396198705.18679-at-qmgate.anl.gov}
"Microscopy-at-Sparc5.Microscopy.Co" {Microscopy-at-Sparc5.Microscopy.Com}
Cc: "Nancy Daerr" {ndaerr-at-mcri.org}
X-Mailer: Mail*Link SMTP-QM 3.0.2

RE} } SEM education 11/9/95

Just a reminder that unless you really need to be certified, there are good
schools that teach SEM technique such as New Paltz and McCrne Research
Institute in Chicago.

--------------------------------------

} To MSA subscribers I am an undergraduate student seeking higher
} education in the fields of SEM and TEM. I will be graduating soon
} and I am searching for a college that will offer certification in
EM.
} Can anyone help me in this area?
}

Everyone knows that the only place to go for EM training is San Joaquin
Delta College in Stockton, CA. They have a fully accredited two year course
for SEM and TEM of biological, or crystalline, materials microscopy. The
address for the school is:
San Joaquin Delta College
Electron Microscopy Lab Holt 121
5151 Pacific Ave.
Stockton, CA. 95207
Tel: (209) 474-5246

The head of the crystalline materials program is Dr. Frank Villalovoz
and the head of the biological materials program is Dr. Judy Murphy. If you
need more information ask around because we graduates of the program are
everywhere.

Good Luck.

Michael W. Odum
Spec. Prep. Tech.
Gatan, Inc.
6678 Owens Dr.
Pleasanton, CA. 94588
Tel: 510-463-0200
Fax: 510-463-0204
E-Mail: modum -at-gatan.com



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From: QENN23A-at-PRODIGY.COM (MR KINGSLEY H MCCROCKLIN)
Date: Thu, 09 Nov 1995 20:57:21 EST
Subject: unscribe

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unscribe qenn23a-at-prodigy.com





From: m.dickson-at-unsw.edu.au (melvyn dickson)
Date: Fri, 10 Nov 1995 12:14:48
Subject: Re: slot grid problems

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To: microscopy-at-Sparc5.Microscopy.Com

In article shaun.sandow-at-anu.edu.au (Shaun Sandow) writes:
} Date: Thu, 09 Nov 1995 16:00:56 +1000
} From: shaun.sandow-at-anu.edu.au (Shaun Sandow)
} Subject: slot grid problems

ways to get better fixing of films onto grids include:

don't use solvents to "degrease" grids. Many solvents leave a residue.
Instead, whisk grids quickly through a small (ethanol burner, low bunsen)
flame so they flash red hot. Too hot and you will know! This is guaranteed
to make them clean and hydrophilic.

Put the film on the DULL side of the grid, which is smoother on the fine scale
and offers better adhesion.

Use cellulose tape glue dissolved off in chloroform. (40 cm of tape in 100 ml
solvent, must NOT be plastic tape, which softens & dissolves a bit) One drop
dried onto each grid before applying the coating film.

Put on the carbon from a carbon arc at an angle to the grid, on the NON coated
side, so the carbon forms a film connecting the bars to the film. Rotate the
grids 180 degrees and put on another coat. That should anchor the film pretty
firmly.






From: jbpawley-at-facstaff.wisc.edu (James Pawley)
Date: Thu, 9 Nov 1995 22:05:33 -0600
Subject: Re: Digital SEMs

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One thing to keep in mind when discussing analog (signal direct to record
CRT) vs. digital (signal digitized first, then sent to CRT of a digital
printer) recording methods is that the sampling process only limits the
performance of the latter method. This is because, according to Nyquist,
you must digitize a single line to 2000 pixels to reliably display data in
which the smallest feature is 1/1000 th of the width of the image in size.
(Not 1/2000!! which would require 4000 pixels/line).

It is true that, in either case, the image is pixellized in the vertical
direction but pixellation makes the size of the smallest displayable
element (i.e. its area in relation to the area of the whole image) about 3
times smaller in a 2500x2500 analog display than it is in a 2000x2000
digital display.

The difference will be most noticeable when recording images at very low
magnification ( {2-5,000, depending on kV and spot size) where the
resolution of the image is not limited by the size of the probing beam.

You should also keep in mind that printing out even a 2000x2000 image on a
300DPI dye-sublimation printer will require a print that is at least 6.6 x
6.6 inches on rather thick paper (which is usually 8.5 x 11 inches), a size
that requires much more storage space than does a Polaroid print (and its
negative, if you don't copy them onto 35mm negs as we do).

Oh, I know that "all storage will be done on disk", but how many big disc
drives can you find now that will play data recorded even 5 years
ago...(assuming that the media is still useable)?

Jim Pawley

} Skipping the HR-Polaroid output.
}
} I would have to agree with Mary, and say don't give up the Polaroid
} yet, but with the following considerations (Which, since I'm not
} intimately familar with the LEO SEM, you should look at):
}
} (1) SEM's with direct analog output to the HR-photo screens, and the
}
} polaroid NEGITIVES are generally capable of 2500 lines of resolution,
}
} at typical SEM photo-rates. 2k x 2k is still alittle less than that
} but you generally never use all 2500 lines on a neg. anyway.
}
} (2) generally printers (1200dpi laser, or 300/600dpi
} dye-sublimation) can not ultimately give the same resolution as the
} polaroids.
}
} (3) On some digital SEM's the output to the HR-photo screen is not
} the original analog signal, but rather the digitally captured image
} at its resolution (1k x 1k or 2k x 2k, more?). Therefore, if you
} send a 1k x 1k digital image to a polaroid HR-photo CRT capable of
} 2500 lines, you will not be getting the full resolution of the
} polaroid anyway so you might want to leave off the polaroid.
}
} (4) Polaroid negs are very useful for a variety of photographic
} imaging needs, which can not be met with affordable digital output
} devices. It would be very useful to not only output current specimen
} images (i.e. specimens in the scope) but also stored images from disk
} to the HR-photo CRT (even images not generated on the scope? or
} after manipulation?) as if the Photo CRT is another digital output
} device like a printer.
} For this a 35mm HR digital output device might serve equally as
} well, and more usefully for other applications (i.e. slides for
} presentations?), however as was just discussed on the list a few
} weeks ago these can be very pricey ($6-10k), but might be an option
} instead of the polaroid [35mm + good grey scale + dye sub printer =
} polaroid output ? $ ?]

****************************************
Prof. James B. Pawley, Ph. 608-263-3147
Room 1235, Engineering Research Building, FAX 608-265-5315
1500 Johnson Dr., Madison, WI, 53706 JBPAWLEY-at-FACSTAFF.WISC.EDU






From: GVKM07A-at-PRODIGY.COM (DR CHARLES A GARBER)
Date: Fri, 10 Nov 1995 03:51:22 EST
Subject: Sample Prep - Polymer Nanoparticles

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-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --

On Nov. 7, Owen P. Mills wrote inquiring about the preparation of
polymer particles in the range of 100-200 nm.

We do a large volume of these kinds of samples in our laboratory:

1] If they are clumping, then probably the concentration of the
suspension is too high and you need a higher dilution by at least one
and maybe two or three orders of magnitude.

2] You want to use an "aerosol" type "duster" valve that incorporates
a capillary tube to disperse the now highly diluted suspension. The
best (but certainly not the only) one I know of is made by Ernest F.
Fullam, Inc. in Schenectady, NY. The cost is less than $100. This is
in all probability the "aerosol" method you mentioned in your posting.

3] You have to worry about the glass transition temperature. If above
room temperature, then you need not further worry. If below room
temperature, then the particles at room temperature will be soft and
you have to worry about the need to "harden" them up. Acrylic
particles can be "hardened" for example by UV using quartz irradiation
tubes.

4] While particles in this range could in theory be done by SEM,
especially cryo SEM, you will find that Pt/C replication TEM will give
much more acceptable results. Use a shadowing angle of 45 degrees.
You can measure the "shadow" and also the apparent diameter, and then
compare the measurements as a insight into any "collapse" of the
particles, being sort of a built in validation that the particles are
indeed hardened into "hard" rigid marbles.

You do not need anything fancy in the way of a vacuum evaporator, any
system giving a vacuum down in the "mid to low 10 to the minus five"
range will give acceptable results.

5] If graft polymer latex, then if you want to see the morphological
details of the graft vs. substrate polymer phase, you will have to use
a still different technique to bring out such structures. But again,
the method actually used will be determined by the specific polymers
present and the kind of reactivity they exhibit.

Chuck

======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail:
GVKM07A-at-prodigy.com
West Chester, PA 19381-0656 USA Customer Service:
SpiSupp-at-aol.com

########################################################
WWW: http://mail.cccbi.chester.pa.us/spi/spihome.html
########################################################
======================================================





From: dshubito-at-d.imap.itd.umich.edu (Dennis Shubitowski)
Date: Fri, 10 Nov 1995 11:00:22 -0500
Subject: Sodium Lamp Replacement Bulb?

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Message-Id: {v02120d00acc927110bba-at-[141.211.157.61]}
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Does anyone know of a source where I can obtain a replacement
bulb for a darkroom sodium vapor lamp? I found one in the
Ted Pella catalogue, but $103 seems a little steep.

Thank you,

Dennis Shubitowski
dshubito-at-umich.edu






From: Ann-Fook Yang :      YANGA-at-em.agr.ca
Date: Fri, 10 Nov 1995 10:24:53 -0500
Subject: Re:slot grid problems--oops

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Message-Id: {s0a33e80.078-at-EM.AGR.CA}
X-Mailer: Novell GroupWise 4.1

An error was made earlier in response to Shaun's question about
slot grid problems. We use 0.3% (not 3%) formvar in chloroform on
beryllium-copper grids. Sorry.





From: ENERGY BEAM SCIENCES, INC :      75767.640-at-CompuServe.COM
Date: 10 Nov 95 11:46:07 EST
Subject: Re: storing gold probes

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Paul webster raised the issue of the proper way to store gold probes. At least
two EM supply companies (one being us) sell colloidal gold probes that have
glycerol in the solution. These probes can be aliquoted and stored indefinitely
in the freezer.
More information on this is available at our WWW site:
http://www.mwrn.com/ebs/ebs.com
Steven Slap, Vice-President





From: chandler-at-lamar.ColoState.EDU (John Chandler)
Date: Fri, 10 Nov 1995 11:42:53 -0700
Subject: Re: Sodium Lamp Replacement Bulb?

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} Does anyone know of a source where I can obtain a replacement
} bulb for a darkroom sodium vapor lamp? I found one in the
} Ted Pella catalogue, but $103 seems a little steep.
}
} Thank you,
}
} Dennis Shubitowski
} dshubito-at-umich.edu

We get replacement bulbs for our Thomas safelights from the photographic
supplier who has a purchasing contract with our university. They cost us
$90, so $103 from Pella doesn't look so bad to me.

John
chandler-at-lamar.ColoState.EDU






From: ENERGY BEAM SCIENCES, INC :      75767.640-at-CompuServe.COM
Date: 10 Nov 95 11:45:44 EST
Subject: Re: Film Processors

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Judy Murphy asked:
"I would appreciate information about processors that can do both paper (RC
papers) and film (EM film 4489, SO163, Kodak 4127 and Ektapan). I am familiar
with the Mohr processor but need to know if there are any other good ones out
there".

Energy Beam Sciences manufactures an automatic EM film processor that works
quite differently from the Mohr instrument. Somef you may have seen in our
booth at the MSA meeting in Kansas City. For more information, see our WWW
page:
http://www.mwrn.com/ebs/ebs.com
or contact me directly.
Steven Slap, Vice-President





From: delannoy-at-welchlink.welch.jhu.edu (Michael Delannoy)
Date: Fri, 10 Nov 1995 13:00:42 -0500
Subject: confocal vs deconvolution microscopy

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Hello Microscopists,
I'd like to get your opinions on making a choice between a
confocal system that can do triple label (green,red and high red
emissions) or a deconvolution system. I realize this may be like comparing
apples to oranges (the latter does not remove out of focus info but utilizes
it), and demos will be conducted for both. So, if you had a wide range of
uses (cultures, sections, yeast etc.) and had to choose one system, what
would it be? We would be interested in both volumetric 3-D and thin optical
sections. All comments are welcome.

Thank you
Mike D





From: Richard Gordon :      gordonr-at-CC.UManitoba.CA
Date: Fri, 10 Nov 1995 08:45:41 -0600 (CST)
Subject: University of Manitoba Professors Academic Freedom Strike Settled

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Mediated negotiations have settled the strike at the University of
Manitoba in Winnipeg, Canada, just in time to "save" the fall semester.
Under the new agreement, individual professors cannot be targeted, and
the faculty will determine which programs (not individuals, as proffered
by the administration), will be eliminated in proven financial
exigencies. Affected individuals would be offered options for
redeployment, retraining, reduced appointment, or early retirement
incentives. This strike of 1000 professors and professional librarians
was a battle over the protection of academic freedom: from the start we
offered cash concessions exceeding the budget shortfall.

We would like to thank you for the hundreds of worldwide e-mail letters,
many reproduced in {http://www.xpressnet.com/umfa} . Internet made a
critical difference in bringing international pressure on our
administration and provincial government (which sided publicly with the
administration), and we are grateful for your support. The issue of
academic freedom was not discussed by the administration until we were 2
weeks into the strike, and your letters arrived. Our students came to
realize that if academic freedom were compromised, their UM degrees would
be worth less. We hope we have all now earned your respect after 3.5
weeks of collegial picketing, with supporters who flew in from
universities across Canada, and with our students and local unions, at
temperatures down to -17C in blowing snow. We hope we have set an example
for the defense of academic freedom worldwide.

Please pass this on to your colleagues as appropriate. Comments may be
sent to us at umfa-at-xpressnet.com, to President
Arnold_Naimark-at-UManitoba.ca, and to Premier Gary Filmon:
premier-at-leg.gov.mb.ca
UMFA = University of Manitoba Faculty Association






From: John W Heckman :      heckman-at-pilot.msu.edu
Date: Fri, 10 Nov 1995 17:10:21 -0500 (EST)
Subject: Re: Sodium Lamp Replacement Bulb?

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Message-Id: {199511102210.RAA99624-at-pilot04.cl.msu.edu}

Dennis:

We've been using off-the-shelf Low pressure lamps in our darkrooms for the
last several safe lamps that have needed replacement. The last one was a
Philips SOX 35 (35 Watts). Cost about $45.00 at our local industrial supply
house. Hasn't fogged anything yet.

cheers,
J.Heckman
heckman-at-pilot.msu.edu
}
}





From: Fiona Graham :      GRAHAM-at-ph.und.ac.za
Date: Fri, 10 Nov 1995 19:00:46 +0200 (SAST)
Subject: EDS system evaluation

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We are in the market for a new energy dispersive x-ray analysis system
to attach to a Hitachi S520 SEM. The systems we are considering
include:

LINK ISIS
NORAN 3050
EDAX DX4
IRIDIUM II microanalysis system.

If any current users of these systems have any comments about the
systems, we would most appreciate hearing from you.

Thanks in advance
Dr Fiona Graham
EM Unit
University of Natal, Durban
South Africa
email: graham-at-ph.und.ac.za
tel: +27 31 2602174 fax: +27 31 2616550




From: MARCFRIEDMAN-at-delphi.com
Date: Fri, 10 Nov 1995 13:24:06 -0500 (EST)
Subject: Time Lapse Vendors

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I have had a request from potential users to compile a list of
vendors that provide software and hardware for time lapse video
microscopy. I would appreciate any help in identifying and
locating current vendors and products, and will gladly share
the listing - please email me directly if you would like a
copy.

TIA.
Marc

Marc M. Friedman, Ph.D.
Marketing Manager
Olympus America Inc.
Precision Instrument Division
2 Corporate Center Drive
Melville, NY 11747-3157
Tel: (516) 844-5039
Fax: (516) 844-5111
email: marcfriedman-at-delphi.com




From: GREG VAUGHN MARTIN :      gmartin-at-welchlink.welch.jhu.edu
Date: Fri, 10 Nov 1995 16:42:02 -0500 (EST)
Subject: Re: LR White Immunolabeling

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Hey folks --
My two cents on the question of gold labeled probes: We have had
excellent results with Jackson (I have no connection with them) IgG gold
(anti- rabbit, mouse, or guinea pig) which seems to be stable for at
least a year at 4C. Since most of the labeling we do eventually involves
localizing two antigens simultaneously, using IgG secondaries fits the
"easiest is best" catagory.

Greg Martin
Dept. of Cell Biology and Anatomy
Johns Hopkins School of Medicine




From: Doug Davis :      doug_davis-at-maillink.berkeley.edu
Date: 10 Nov 1995 09:33:48 -0800
Subject: Re: Slot grid substrates

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Message-ID: {n1396126416.78973-at-maillink.berkeley.edu}

REGARDING RE} Slot grid substrates

} ... For the past few months I've been having considerable trouble with
} what appeared to be focus problems. It now appears that the film is
} "shifting". The film is carbon stabilised and clean; we've tried } modifying
formvar coating preparations, carbon coating and specimen } storage, but the
film movement is still present. It appears "tight" } when on the grid, but
appears to shift under the beam (as indicated by } what appeared to be
focussing problems). Anyone with a } suggestion????????????????????????/

Shaun, Is the movement only seen on slot grids or do you see on small mesh
(200) also? The carbon stabilization should have done the trick and I am just
wondering if the holder itself is unstable in some way.
If focusing is the issue and not lateral movement, one VERY long shot
could be the stability of the objective lens current. Sweeping the
potentiometer knob repeatedly through the focus range can help clear dirt from
the contacts and a shot of electrical contact cleaner behind the panel should
be attempted before moving to more costly diagnostics. After all other
low-cost options are exhausted, call for service.

Good luck!
doug_davis-at-maillink.berkeley.edu





From: beth-at-dogwood.botany.uga.edu (Beth Richardson)
Date: Fri, 10 Nov 1995 15:30:23 -0500
Subject: CPD tissue baskets

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Message-Id: {199511102027.PAA07419-at-dogwood.botany.uga.edu}
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I'm looking for tissue baskets that were/are used in the Polaron Critical
Point Dryer. Ted Pella use to sell them (page 50 of their old catalog) as
item # 1215-2, Tissue baskets only; set of 3. We use the baskets in our
plunge freezing set-up and after a period of time they sprout legs and walk
away from the lab...never to return! Ted quit selling them. Any
suggestions? I will try to provide a good home for any old tissue baskets
you may have stuffed away in a drawer and no longer need.

Best regards,

Beth Richardson
EM Lab Coordinator
Botany Department
University of Georgia
Athens, GA 30602-7271






From: beth-at-dogwood.botany.uga.edu (Beth Richardson)
Date: Fri, 10 Nov 1995 15:30:23 -0500
Subject: CPD tissue baskets

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Message-Id: {199511102027.PAA07419-at-dogwood.botany.uga.edu}
Mime-Version: 1.0
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I'm looking for tissue baskets that were/are used in the Polaron Critical
Point Dryer. Ted Pella use to sell them (page 50 of their old catalog) as
item # 1215-2, Tissue baskets only; set of 3. We use the baskets in our
plunge freezing set-up and after a period of time they sprout legs and walk
away from the lab...never to return! Ted quit selling them. Any
suggestions? I will try to provide a good home for any old tissue baskets
you may have stuffed away in a drawer and no longer need.

Best regards,

Beth Richardson
EM Lab Coordinator
Botany Department
University of Georgia
Athens, GA 30602-7271






From: Helen.Hassander-at-polymer.lth.se (Helen Hassander)
Date: Mon, 13 Nov 1995 16:27:30 +0200
Subject: Re:Sample prep - polymer nanoparticles

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X-Sender: Helen.Hassander-at-mail.chemeng.lth.se
Message-Id: {v01510100acccca8cd1b2-at-[130.235.86.65]}
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As Dr Charles A Garber wrote there are special problems to image polymers
in TEM. If you are not a polymer scientist, the negative staining method is
the most safe one. It will give a proper result even if you have a film
forming (low glass transition) or beam sensitive polymer. When you have
learnt to dilute the latexes and add appropriate amount of Uranyl Acetate
or PTA, it is also very easy to use.
Helen Hassander
Polymer group, Lund University






From: BAIER-at-ubvms.cc.buffalo.edu
Date: Sat, 11 Nov 1995 21:58:56 -0500 (EST)
Subject: Microscopy

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subscription request from bob baier at SUNY Buffalo




From: baskin-at-biosci.mbp.missouri.edu (Tobias Baskin)
Date: Mon, 13 Nov 1995 11:09:12 -0600
Subject: LM/ Hg HBO 50W lifetimes

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Message-Id: {v01520d00accd2b1877c1-at-[128.206.15.200]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Greetings,
How long are folks out there running their 50 Watt Mercury arc
lamps? I ask because we used to run the 100 W arc's for 400 to 500 h, which
is double or more than the manufacturer's guarantee of 200 h. The 50 W arc
lamps are only guaranteed for 100h, which comes around awefully quickly.
Any comments??

Thanks,
Tobias Baskin

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
___ ____ ^ ____ _____ Tobias I. Baskin
/ \ / / \ / \ / University of Missouri
/ | / / \ / / Biological Sciences
/___ / /__ /_____\ / /__ 109 Tucker Hall
/ / / \ ( / Columbia, MO 65211 USA
/ / / \ \ / voice: 314-882-0173
/ /____ / \ \____/ /_____ fax: 314-882-0123






From: GVKM07A-at-PRODIGY.COM (DR CHARLES A GARBER)
Date: Mon, 13 Nov 1995 01:37:49 EST
Subject: CPD Tissue Baskets

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-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --

On Nov. 10, Beth Richardson asked about the CPD baskets once made by
Polaron (e.g. their #1215-2). The last I heard, this product was
available from Energy Beam Sciences, the US distributor for Fisons
Instruments VG Microtech based in the UK.

As an alternative, SPI has made for some years our microporous specimen
capsules in pore sizes of either 120um or 78 um. So far as I know,
they have been used quite successfully in your application and with the
advantage of there being far less of a chance of sample cross-
contamination. See our web site (below) for further information under
"Current WWW Specials". If still in doubt, e-mail me for further
information and a sample pack for evaluation.

Chuck

======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail:
GVKM07A-at-prodigy.com
West Chester, PA 19381-0656 USA Customer Service:
SpiSupp-at-aol.com

########################################################
WWW: http://mail.cccbi.chester.pa.us/spi/spihome.html
########################################################
======================================================







From: STEELE-at-KRDC.INT.ALCAN.CA
Date: Mon, 13 Nov 1995 14:17:43 -0500 (EST)
Subject: EPOL TEM X-sections

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--Boundary (ID qMMdyj3hoMjMtH004Fc9Rg)
Content-type: TEXT/PLAIN

Thanks to everyone who responded to my call for suggested ways to prepare
thin metal X-sections by electropolishing.

Naturally I assumed that EVERYONE would know which alloy system I'm working
with, so I didn't include that bit of information. I'll let you in on the
secret now though, we're talking aluminum.

To recap the responses:

Microtomy was suggested, but is unfortunately not an option due to the
deformation it induces.

I was urged not to give up on tripod polishing (and I won't), but the boss
wants to do away with the whole ion milling thing for this aplication (if
possible).

A couple of individuals with either a better memory than mine, or a better
database, not only remembered the combo Epol / Dimple experiment, but gave me
a reference to check out.

Encapsulating in low temp melting metals was suggested. I've had some bad
experiences with Pb / Bi in the TEM, so I'm a bit leary, but it's worth a
look.

And the likely winner, to my mind, electrolytic or electroless coating.

I've actually had some good success in the past with electroless coating Ni
on Al powders and ceramic particles, followed by ion milling. It wasn't much
fun though (tended to coat everything but the bits of interest), and was
awfully slow (maybe just my setup). I've yet to try Epolishing it either.

** The questions of the day then, to those of you in the know, is:

1. Can electrolytic coating be carried out as fast as electroless (and how)?

2. What metals would you suggest based on either speed or best chance to
polish in the same electrolyte as Aluminum? Is Ni the best option?

Thanks in advance,

###############################################################
# #
# Don Steele STEELE-at-KRDC.INT.ALCAN.CA #
# ALCAN INTERNATIONAL #
# Kingston Research and Development Center #
# P. O. Box 8400 #
# Kingston, Ontario Canada K7L 5L9 #
# #
###############################################################

--Boundary (ID qMMdyj3hoMjMtH004Fc9Rg)--




From: krogers-at-ecn.purdue.edu (Kirk Rogers)
Date: Mon, 13 Nov 1995 15:39:31 -0500
Subject: Edington TEM Monographs

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Message-Id: {v01530500accd5c499836-at-[128.46.155.231]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Does Anyone know where I can get a copy of the "Monographs in Practical
Electron Microscopy in Materials Science" parts 1-4, by J. W. Edington?

They were origonally published by the Philips Technical Library/MacMillan,
but I heard the series was out of print.

Any suggestions would be helpful.

Thanks,


-Kirk
________________________________________________
Kirk A. Rogers
krogers-at-materials.ecn.purdue.edu
317-494-8751 office http://materials.ecn.purdue.edu/~krogers
317-494-1204 FAX
Purdue University, School of Materials Engineering,
1289 MSEE building, W. Lafayette, IN 47907-1289






From: R.G.White-at-sci.monash.edu.au (Rosemary White)
Date: Tue, 14 Nov 1995 08:20:59 +1200
Subject: Re: LM/ Hg HBO 50W lifetimes

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Hi Tobias,

We've run our last two 50W HBO Hg lamps for 1) just under 300 h (a mistake!
- changed when the fluorescence got too dim) and 2) just over 200 h. As
long as they don't get turned on and off too frequently they seem OK. It
gives our Leica rep kittens, though.

cheers, Rosemary


____________________________________________________________
Rosemary White __ /
Department of Ecology _/ \__/ \
and Evolutionary Biology / \
Monash University / Australia \
Clayton, Victoria 3168 \ ____ /
phone 61-3-9905 5670 \_/ \_*_/
fax 61-3-9905 5613 __
email r.g.white-at-sci.monash.edu.au \/





From: ENERGY BEAM SCIENCES, INC :      75767.640-at-CompuServe.COM
Date: 13 Nov 95 09:17:57 EST
Subject: Re: Polaron CPD tissue baskets

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Beth Richardson asked:
"I'm looking for tissue baskets that were/are used in the Polaron Critical Point
Dryer. Ted Pella use to sell them (page 50 of their old catalog) as item #
1215-2."
Energy Beam Sciences has been, for almost two years, the exclusive U.S.
distributor for Polaron equipment (and the authorized service representative).
The tissue baskets, along with other supplies (targets, etc) are available,
usually from stock.
Please contact me directly for details.
Steven Slap, Vice-President





From: andreas.brech-at-bio.uio.no (Andreas Brech)
Date: Mon, 13 Nov 1995 12:40:53 +0100
Subject: SV40 antibody

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Message-Id: {199511131143.MAA10537-at-darwin.uio.no}
X-Sender: abrech-at-darwin.uio.no
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Dear colleagues

Does anybody out there know about antibodies against SV40 (simian virus 40).
The antibody should recognize the virus, not the simian virus 40 T antigen
(SV 40 Tag). The antibody is needed for immunocytochemistry on the EM-level.
Any information about researchers or commercial suppliers is greatly
appreciated.

Thanks



Andreas Brech
Electron Microscopical Unit for Biological Sciences
Department of Biology, University of Oslo.
P.O.Box 1062 Blindern
N-0316 Oslo 3
Norway
Tel.: + 43-22 85 61 89 (work)
+ 43-22 43 83 23 (privat)
Fax.: + 43-22 85 47 26
e-mail.: abrech-at-bio.uio.no





From: Rolf Odselius :      Rolf.Odselius-at-emu.lu.se
Date: Mon, 13 Nov 1995 10:11:26 +0100
Subject: Change of WWW address!

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Guncar-at-Carlstedt.se, jd-at-ana.aau.dk, Leif.Ejderhamn-at-mailbox.swipnet.se,
Leif.Odselius-at-micronic.se, Matthew.Ellis-at-csb.ki.se,
ZALUZEC-at-aaem.amc.anl.gov, syspa-at-fy.chalmers.se, pk-at-gambro.se

The address to the WWW pages of the

Electron Microscopy Unit
Medical Faculty
Lund University
SWEDEN

has changed (again!). I beleive that this new address will keep for
several years.
The old URL was: http://www.medfak.lu.se/medinst/emu/

____________________________
| |
| THE NEW URL IS: |
| |
| http://www.emu.lu.se/ |
|____________________________|

Please change your address books and links. Sorry for the
inconveniance!

With best regards
Rolf Odselius
____________________________________________________________________
Rolf Odselius, PhD |Rolf.Odselius-at-emu.lu.se
Electron Microscopy Unit |Phone: +46 46 171075 office
University Hospital | +46 46 171155 lab
S-221 85 Lund, Sweden | +46 46 293692 home
| +46 10 6705655 mobile
http://www.medfak.lu.se/medinst/emu/ |Pager: +46 740 288992
SCANDEM: http://www.ldc.lu.se/~scandem/|Fax: +46 46 172975




From: Colin Veitch :      C.Veitch-at-geel.dwt.csiro.au
Date: Sun, 12 Nov 1995 17:51:26 -0600
Subject: EELS Simulation

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Hi all,

Does anyone out there know of a simulation package for generating EELS
spectra? NIST's DTSA does this for EDXS and I was just wondering about the
possibility of one existing for EELS.

Many thanks in advance.


Colin V.
###############################################################################
# #
# Colin J. Veitch C.Veitch-at-geel.dwt.csiro.au #
# Instrumentation Scientist #
# CSIRO Division of Wool Technology Tel. +61 (0) 52 275611 #
# P.O. Box 21 Fax. +61 (0) 52 275657 #
# BELMONT Vic 3216 #
# Australia #
# #
# "We see the Universe the way it is because if it were different, we would #
# not be here to observe it." #
# #
###############################################################################





From: Adriana Pinheiro Martinelli Rodriguez :      adriana-at-aguia.cena.usp.br
Date: Sun, 12 Nov 1995 18:20:13 -0300 (BST)
Subject: Re: mounting media

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Posted-Date: Sun, 12 Nov 1995 18:20:13 -0300 (BST)
Received-Date: Sun, 12 Nov 95 18:20:14 BST

I have used "mount-quick" and found good results with it. There are two
types:
Mount quick solvent base
Mount quick water base
I've used the first one, and it is carried by EMS (fax # 215-646-8931 US).
Hope it helps
Adriana Rodriguez
CENA/USP/Brazil

On Wed, 8 Nov 1995, Rosemary White wrote:

} Dear all,
}
} While in Germany a few years ago, a colleague bought a xylene-based medium
} called Melanol for making permanent (or at least semi-permanent) mounts.
} She has just about run out but can't find a supplier to buy more. Is there
} a new supplier of this mountant? If not, can anyone advise re. a suitable,
} less toxic substitute?
}
} TIA,
} Rosemary White
}
}
}
} ____________________________________________________________
} Rosemary White __ /
} Department of Ecology _/ \__/ \
} and Evolutionary Biology / \
} Monash University / Australia \
} Clayton, Victoria 3168 \ ____ /
} phone 61-3-9905 5670 \_/ \_*_/
} fax 61-3-9905 5613 __
} email r.g.white-at-sci.monash.edu.au \/
}
}




From: Daniel Possin :      oemlab-at-u.washington.edu
Date: Sat, 11 Nov 1995 18:57:45 -0800 (PST)
Subject: Re: LR White immunolabeling-LM/EM

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Hi Pat -

I'm sorry to tell you that antibodies can 'go bad' when "stored properly".
It has been our experience that different antibodies exihibit different
storage stabilities. The type of storage (eg. frozen w/ glycerol, storage
where exposed to light or storage in "frost free" freezers) can also make
a big difference. Even if you have done everything right, some antibodies
just don't seem to store well. When you tell me that other antibodies are
working well with your techniques, it seems likely to me that your
antibody has degraded. Sorry to be the bringer (or confirmer) of bad news.

Dan


On Wed, 8 Nov 1995, Pat Masarachia wrote:

} Date: Wed, 8 Nov 1995 16:11:54 EST
} From: Pat Masarachia {pat_masarachia-at-Merck.Com}
} To: Microscopy-at-Sparc5.Microscopy.Com
} Subject: LR White immunolabeling-LM/EM
}
} There is some rule that when something is going well don't change
} or stop anything.. for several months I had good success immuno-
} gold labeling using a polyclonal antibody for an integrin antigen. I
} could get specific labeling in .3 micron sections of bone embedded
} in LR White (silver intensified gold) for LM or in thin LR White
} sections for EM. The same specific labeling results occurred in a
} dozen experiments; antigen absorption of the antibody and deletion
} of antibody were negative controls. The antibody was specific and
} dependable. I had to interrupt the project and did not return for
} a year. Murphy's law takes over. With the same antibody, using
} aliquots stored at -20 C as per manufacturers instructions as well
} as aliquots stored at -70 C, I cannot get labeling on sections
} cut from the original blocks. The antibody still works on pre-
} embedded tissue culture. On top of this, the original manufacturer
} went out of business. The antibody produced by a new company with
} the original protocol does not work. I thought that maybe the LR
} White blocks after a year further polymerized to reduce antigenicity.
} I worked up newly embedded tissue, freshly cut sections, and still
} got no label. I've tried different secondary antibodies and different
} blocking and different buffers -- no label. A different antibody for a
} different antigen does specifically label in sections from old
} blocks or new. So... can an antibody 'die' even if stored properly?
} Sorry for this long winded tale but with no substitute available
} commercially for this particular antibody I'd love to know what
} happened.
} Thanks for any comments.
}
} Pat Masarachia
} Bone Biology
} Merck Research Laboratories
} West Point, PA 19486
}
}
}

%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
% %
% Daniel Possin Work: 206/ 543-7489 %
% Dept. of Ophthalmology RJ-10 FAX: 206/ 543-4414 %
% University of Washington Home: 206/ 778-1714 %
% Seattle WA 98195 USA Email: oemlab-at-u.washington.edu %
% %
% "The chinese expression 'cheung meng ba sui, gong hey fat choy' is %
% equilvalent to Vulcan expression 'live long and prosper'. It's a %
% small universe and getting smaller everyday". %
% %
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%





From: Daniel L. Callahan :      dlc-at-owlnet.rice.edu
Date: Sun, 12 Nov 1995 12:01:51 -0600 (CST)
Subject: Current resolution specs for TEM image recording

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Hello All:

There have been several running commentaries regarding resolution of various
recording methods. I thought that I would ask the various vendors and
others in the know what are the product specified resolutions AND
recording areas of the following:

standard film (e.g. SO 163)
CCD
video cam

I was particularly interested in product specifics since they tend to be
conservative estimates. I am also interested in how people think that
these resolutions should be compared.

Daniel L. Callahan
Department of Mechanical Engg. and Materials Science
Rice University
dlc-at-owlnet.rice.edu




From: lmiller-at-ux1.cso.uiuc.edu (Lou Ann Miller)
Date: Sat, 11 Nov 1995 14:03:59 -0500
Subject: Re: storing gold probes

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Message-Id: {v01510100accaa33878cb-at-[128.174.23.126]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Steven Slap writes:

At least
two EM supply companies (one being us) sell colloidal gold probes that have
glycerol in the solution. These probes can be aliquoted and stored indefinitely
in the freezer.



Hello Steven,

Just a question about the storage of your gold probes in glycerin.

Then..... do you actually run the procedure with glycerin in the probe, or
is there a clean up procedure after thawing the probe?

Have you found the glycerin to interfere with anything?

Thanks,
Lou Ann

***************************
Lou Ann Miller
Microscopic Imaging Lab
College of Vet. Medicine
University of Illinois
2001 S Lincoln Ave
Urbana,Illinois 61801
217-244-1566
lmiller-at-ux1.cso.uiuc.edu

Microscopy Home Page:
http://www.cvm.uiuc.edu/MicImagLab/MicImagLab.html

Personal Home Page:
http://www.cvm.uiuc.edu/HomePages/LouAnnMiller/Homepage.html






From: John W Heckman :      heckman-at-pilot.msu.edu
Date: Fri, 10 Nov 1995 17:15:14 -0500 (EST)
Subject: Re: Sodium Lamp Replacement Bulb?

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Dennis:

We've used regular off-the shelf Na lamps for just that reason. The Philips
SOX 35 fits the Thomas safe light housing and looks nearly the same as the OE
lamp. They are about $45.00 at our local industrial supply house. Hasn't
fogged anything yet.

Cheers,
John
heckman-at-pilot.msu.edu

} } } Does anyone know of a source where I can obtain
a replacement } } bulb for a darkroom sodium vapor lamp? I found one in the
} } Ted Pella catalogue, but $103 seems a little steep.
} }
} } Thank you,
} }
} } Dennis Shubitowski
} } dshubito-at-umich.edu
}
} We get replacement bulbs for our Thomas safelights from the photographic
} supplier who has a purchasing contract with our university. They cost us
} $90, so $103 from Pella doesn't look so bad to me.
}
} John
} chandler-at-lamar.ColoState.EDU
}
}
}





From: Paul Webster :      Paul.Webster-at-quickmail.yale.edu
Date: 10 Nov 1995 15:45:47 -0400
Subject: R>storing gold probes

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Message-Id: {n1396103720.9152-at-QuickMail.Yale.edu}

Subject: Time: 3:36 PM
OFFICE MEMO R} storing gold probes Date: 11/10/95

I agree that it is a good idea to store colloidal gold probes in 50% glycerol.
They can be left at -20#161#C for long periods without much loss of activity.
As far as I know there has been no quantitative study on the effects of long
term storage under these conditions on labeling efficiency. Once cryoprotected,
the probes could also be stored at -80#161#C or in liquid nitrogen. I know of
one report where colloidal gold probes were successfully freeze dried and
reconstituted without harm (Lucocq and Baschong, some time in the '80's. They
used lectin-gold conjugates.
Does anybody know what the effects of long exposure to glycerol at RT has on
gold probes and antibodies? I ask because I send antibodies and gold probes by
mail in 50% glycerol.





From: Benjamin Walcott :      bwalcott-at-ccmail.sunysb.edu
Date: Mon, 13 Nov 1995 18:02:13 -0500
Subject: Re: LM/ Hg HBO 50W lifetimes

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In {v01520d00accd2b1877c1-at-[128.206.15.200]} , Tobias Baskin wrote:
} Greetings,
} How long are folks out there running their 50 Watt Mercury arc
} lamps? I ask because we used to run the 100 W arc's for 400 to 500 h, which
} is double or more than the manufacturer's guarantee of 200 h. The 50 W arc
} lamps are only guaranteed for 100h, which comes around awefully quickly.
} Any comments??
}
} Thanks,
} Tobias Baskin
}
} - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

I find that I can run the 50 watt mercury arcs for about 170-190 hours
before I find that they either begin to flicker or the intensity of the
staining that I am using them to detect seems to drop in intensity. I have
also been told, probably by an arc lamp salesperson, that if you run them too
long beyond their normal life, they can explode. I have never had that
happen in the 10 or more years that I have been using them.


Benjamin Walcott
Dept of Neurobiology and Behavior
University at Stony Brook
Stony Brook, NY 11794




From: GVKM07A-at-PRODIGY.COM (DR CHARLES A GARBER)
Date: Sat, 11 Nov 1995 11:32:53 EST
Subject: JEOL 2000FX arcing

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-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --

There has been some discussion on the subject of "Freon" being used in
the HT tank. Of course, technically, there is no such thing called
"Freon" being made any longer by DuPont (they still make this class of
products but have given them different names). And because of
environmental concerns, I don't think there is any more of the R-12
being produced either, by anyone, and in order to ship what is
remaining in inventory,one almost needs an act of Congress to legally
ship the R-12 over international boundaries. It was the R-12 that was
originally used in the HT tanks.

We would advise against using either R-12 or any so-called replacements
from the little "duster" sized cans (even though our firm has been a
major supplier of such products) and that if these fluorocarbon
materials are going to be used, then they should be procured only in
the larger size containers, such as the 25 Kg container (not offered by
SPI Supplies) referred to in a previous posting. Here is the main
reason:

The "duster" type cans use at least one and sometimes two sets of
internal "O" rings to seal the seams of the cans. These "O" rings, to
give them the needed elastomeric properties are plasticized, and over
time, the liquid inside leaches out some of this plasticizer. Many of
us have done the "clean duster" test by turning the duster upsidedown
and expelling against a clean glass surface and once the frost and
water disappears, an easily observable film remains. It is this
hydrocarbon film that could very well be causing the problem. And
since there are no such "O" rings (so I am told) in the larger
containers, that is why the problem seems to not show up when the
larger container is used.

Apparently also, hydrocarbon contaminating oils can creep into the cans
from the use of oil sealed compressors in the filling line. However,
this source can be successfully controlled by having cans filled in
lines for which this is never a problem. This also gives me the chance
to point out that duster cans promoted, for example, for blowing
emergency horns or chilling cocktain glasses, in general are not filled
to the same standard of starting purity, and in general are not going
to be the same quality product you would get from a reputable EM
consumables supplier. Having said that however, the controlling factor
is the leaching of plasticizer from the "O" rings.

I don't know about the moisture explanation, but it seems to be
"accepted" that any level of hydrocarbon contaminant in the liquid in
the HT tank is quite deleterious and that is why we have for some years
advised against the use of these little cans for this purpose. Just
how much plasticizer is going to be present is going to be a function
of shelf life after filling, and it is also going to depend on the
thermal history of the can during storage and shipment. However if
someone has on their shelf any of the R-12 duster type cans, and is
contemplating their use for this purpose, remember they were probably
filled on the order of ten years ago (or more). My guess is that they
would have for the HT tank application an unacceptable level of
hydrocarbon contamination.

Chuck

======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail:
GVKM07A-at-prodigy.com
West Chester, PA 19381-0656 USA Customer Service:
SpiSupp-at-aol.com

########################################################
WWW: http://mail.cccbi.chester.pa.us/spi/spihome.html
########################################################
======================================================







From: Nina S Allen :      nallen-at-unity.ncsu.edu
Date: Mon, 13 Nov 1995 13:12:39 -0500 (EST)
Subject: Re: confocal vs deconvolution microscopy

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Dear Mike D.
This is a very timely question: deconvolution vs confocal.
I have to make the same choice ... to some extent.

I have had a Scanalytics deconvolution demonstration and have also seen
Deltavision. As you know, Scanalytics is based on the Fay and Carrington
studies and
Deltavision uses modified Aagard and Sedat mathematics. Both are good
and one would have to decide which method you prefer.
I have a Metamorph system (Universal Imaging) and found out that for a
reasonable sum I can add the Scanalytics software to the system. You can
use that after confocal imaging (of course defeating the usefulness of
using many wavelengths and low intensity) but it may further sharpen your
images. If you have a microscope with an xyz controlled stage, you can
use the metamorph system to deconvolve (with Scanalyticson its. You now
need a powerful PC, which could run overnight .... to deconvolve the
images... and you will need a cooled CCD to collect the images.

I do believe there are times when the confocal microscope will be
superior to the deconvolution and in reverse. I have a large multiuser
facility in which to place the confocal and / or deconvolution equipment
and have to have a system that is userfriendly and can give fairly quick
results. I am fortunate in that I have a set-up on which I can test the
Scanalytics for a fairly reasonable price. If it proves to be really
useful, I will apply for funding for a faster computer, etc.

It is not an easy choice, but I believe if there is no confocal in the
area, it is very useful and the best choice. What do others think?

Nina Allen




From: pbarnett-at-crl.com (Peter D. Barnett)
Date: Mon, 13 Nov 1995 16:43:00 -0800
Subject: Re:Sample prep - polymer nanoparticles

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There is an article in the latest issue of THe Microscope that might be of
interest: "Cryo-Ultramicrotomy of Individual Latex Particles for Examination
of Internal Morphology", Marcelli, Angela. The Microscope 43(3):117-120(1995)

Peter D. Barnett
Forensic Science Associates
e-mail: pbarnett-at-crl.com





From: jr :      jeanross-at-emiris.iaf.uiowa.edu
Date: Mon, 13 Nov 1995 15:21:35 -0600 (CST)
Subject: Re: SEM education

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Excuse me, but Delta is NOT the only school for EM training. I am a
graduate of Madison Area Technical College and they offer a two-year
Associate Degree program in electron microscopy. MATC grads are also
everywhere!

Jean Ross
Research Assistant
Central Electron Microscopy Research Facility
Univ. of Iowa
Iowa City, IA 52242
319-335-8142


On Thu, 9 Nov 1995, Michael Odum wrote:

} On 11/7/95 Bridgett Byrnes sent:
}
} } To MSA subscribers I am an undergraduate student seeking higher
} } education in the fields of SEM and TEM. I will be graduating soon
} } and I am searching for a college that will offer certification in EM.
} } Can anyone help me in this area?
} }
}
} Everyone knows that the only place to go for EM training is San Joaquin
} Delta College in Stockton, CA. They have a fully accredited two year course
} for SEM and TEM of biological, or crystalline, materials microscopy. The
} address for the school is:
} San Joaquin Delta College
} Electron Microscopy Lab Holt 121
} 5151 Pacific Ave.
} Stockton, CA. 95207
} Tel: (209) 474-5246
}
} The head of the crystalline materials program is Dr. Frank Villalovoz
} and the head of the biological materials program is Dr. Judy Murphy. If you
} need more information ask around because we graduates of the program are
} everywhere.
}
} Good Luck.
}
} Michael W. Odum
} Spec. Prep. Tech.
} Gatan, Inc.
} 6678 Owens Dr.
} Pleasanton, CA. 94588
} Tel: 510-463-0200
} Fax: 510-463-0204
} E-Mail: modum -at-gatan.com
}






From: William Tivol :      tivol-at-wadsworth.org
Date: Mon, 13 Nov 1995 15:03:20 -0500 (EST)
Subject: Re: LM/ Hg HBO 50W lifetimes

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} How long are folks out there running their 50 Watt Mercury arc
} lamps? I ask because we used to run the 100 W arc's for 400 to 500 h, which
} is double or more than the manufacturer's guarantee of 200 h. The 50 W arc
} lamps are only guaranteed for 100h, which comes around awefully quickly.
} Any comments??

Dear Tobias,
I had some experience with a 1 kW high-pressure Hg lamp which may
be relevant to your question. It too was rated at 100 hrs, and the mfr
told me the usual method of failure was explosive! I set up the lamp and
ran it continuously for } 400 hrs, and it was still good when I turned it
off. The biggest contribution to failure comes from thermal stress, so if
the lamp is not turned on and off, but is stabile at a particular tempera-
ture, it will last a long time.
The lamps usually fail just after they are turned on for one time
too many. This happened to me just as I reached for the air vent on the
lamp housing. I was startled but uninjured, but the lamp took out the
reflector, damaged a lens and put several dents in the housing.
If you can arrange your work so that it can all get done in one
stretch, you should not be afraid to try using the lamp for 400-500 hrs,
but if you are using it in equipment which is turned on only when in use,
change the lamp when the mfr says, unless you have a surplus of housings and
a shortage of lamps. Good luck.
Yours,
Bill Tivol




From: Daniel Possin :      oemlab-at-u.washington.edu
Date: Sat, 11 Nov 1995 19:15:49 -0800 (PST)
Subject: Re: slot grid problems

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Message-Id: {9511121652.AA05613-at-carbon60.fysik.dtu.dk}

Hi Shaun -

1x2 mm slot grids are notoriously hard to completely stabilize even when
carbon coated. If you are sure of your coating technique, I would begin
viewing each new grid by spreading the electron beam just wider than the
length of the grid slot and irradiate the whole thing for about 10-15
minutes prior to taking a closer look. This will strip the light elements
out of the formvar film and "carborize" it making it more stabile. If
this still is not enough I would consider making thicker films by
increasing the concentration of your formvar solution. 1% Formvar in
chloroform seems a little thin to me even with carbon coating. If you
still have trouble, e-mail me again.

Dan

On Thu, 9 Nov 1995, Shaun Sandow wrote:

} Date: Thu, 09 Nov 1995 16:00:56 +1000
} From: Shaun Sandow {shaun.sandow-at-anu.edu.au}
} To: microscopy-at-Sparc5.Microscopy.Com
} Subject: slot grid problems
}
} Dear Microscopists,
}
} I'm looking at serial sections of nerves on formvar (1% in chloroform)
} coated slot copper grids (pretty standard stuff). The grids are acetone and
} distilled water cleaned several times and coated as per standard (which has
} worked consistently well for 4 years). For the past few months I've been
} having considerable trouble with what appeared to be focus problems. It now
} appears that the film is "shifting". The film is carbon stabilised and
} clean; we've tried modifying formvar coating preparations, carbon coating
} and specimen storage, but the film movement is still present. It appears
} "tight" when on the grid, but appears to shift under the beam (as indicated
} by what appeared to be focussing problems). Anyone with a
} suggestion????????????????????????/
}
} Thanks,
} ______________________________________________
}
} Shaun Sandow,
} Division of Neuroscience,
} John Curtin School of Medical Research,
} Australian National University,
} ACT 0200
}
} Ph. (06) 249 4782
} Fax. (06) 249 2687
}
}

%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
% %
% Daniel Possin Work: 206/ 543-7489 %
% Dept. of Ophthalmology RJ-10 FAX: 206/ 543-4414 %
% University of Washington Home: 206/ 778-1714 %
% Seattle WA 98195 USA Email: oemlab-at-u.washington.edu %
% %
% "The chinese expression 'cheung meng ba sui, gong hey fat choy' is %
% equilvalent to Vulcan expression 'live long and prosper'. It's a %
% small universe and getting smaller everyday". %
% %
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%





From: jbpawley-at-facstaff.wisc.edu (Jim Pawley)
Date: Fri, 10 Nov 1995 19:24:57 -0600
Subject: Re: Digital SEMs

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Dear Warren,

I can assure you that Nyquist does apply to the sampling of images.
Usually Nyquist is set by the spatial frequencies transmitted by the optics
but in any case, Nyquist defines the smallest spatial feature that will be
reliably preserved in digital data.

One is apt to think that one will be able to record a small feature as long
as it is larger than the size of a pixel. If the feature happens to be
situated so that its centroid is in the center of a pixel you will indeed
record it (supposing perfect digitizing , something else that doesn't
always happen). However, in general, it will not be so conveniently
situated (if the feature lands on the border line, you will end up
recording a feature that is twice as wide as it should have been and only
half as bright) and the oversampling factor of 2 (2.3 actually)
substantially improves the odds (at least if the contrast varies with
spatial freguency in a smooth and reasonable manner).

The SEM is an interesting case in that this last condition is often not
met. Although at high mag, the resolution in most recorded images is much
more likely to be limited by too high a beam voltage or too large an
electron beam (so Nyquist is irrelevant), the immense magnification range
of the SEM, coupled to the fact that the beam diameter need not change
with magnification, means that this is not true at low mag. Given a slow
enough scan time (to provide more data for a good S/N for more pixels) and
a good enough record screen (many do not really have the 2500 lines that
they claim but 10,000 x10,000 is possible and ideed was once offered by
Zeiss), the image bandwidth can greatly exceed the Nyquist frequency of any
reasonable digitizer (by 10-100x). For instance, it is quite possible for
adjascent pixels to be black then white, something that does not happen
with a signal having a bandwidth near the Nyquist frequency.

Fortunately, this is not a major problem because, if you need 100 sec to
get 2nm data from area 1000 nm on a side (100kx), then you will require
10,000 sec to acquire such data from an area 10,000 nm on a side (10,000x)
and 1,000,000 sec to do the same at 1,000x and so one at lower mags. The
fact that no one wants to wait this long (let alone the stability and
focusing problems) means that the fact that we really don't have any way to
store or display such data in not a problem.

Jim Pawley

****************************************
Prof. James B. Pawley, Ph. 608-263-3147
Room 1235, Engineering Research Building, FAX 608-265-5315
1500 Johnson Dr., Madison, WI, 53706 JBPAWLEY-at-FACSTAFF.WISC.EDU






From: martin krejci :      martin-at-a.iap.phys.ethz.ch
Date: Tue, 14 Nov 1995 05:06:22 GMT
Subject: unsubscribe

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UNSUBSCRIBE martin-at-a.iap.phys.ethz.ch

______________________________________________________________________________

Martin Krejci {martin-at-a.iap.phys.ethz.ch}

AFIF Institute of Private:
Applied Physics

ETH Gebaeude, Technopark ETH Hoenggerberg Schuetzenmatt 17
Pfingstweidstr. 30 HPT D19 8046 Zuerich
CH-8005 Zuerich CH-8093 Zuerich

Phone: Phone: Phone:
+41(1) 445 14 78 +41(1) 633 67 92 +41(1) 371 68 74
Fax: Fax:
+41(1) 445 14 99 +41(1) 633 11 05
______________________________________________________________________________




From: Ronald LHerault :      lherault-at-acs.bu.edu
Date: Tue, 14 Nov 1995 08:13:49 -0500 (EST)
Subject: Rabbit antibodies

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A student here at the dental school is doing a project a bit removed from
our normal lines of research. He needs to find a source for Anti-rabbit
Alkaline Phosphatase primary antibody (monoclonal) and/or Anti-rabbit
osteocalcine Ab (monoclonal) if I am reading his writing correctly.

So far he has not been able to find a source from the catalogs we have.
Does anyone know where we can git htese materials?

Ron




From: Richard W. Fonda :      fonda-at-anvil.nrl.navy.mil
Date: Tue, 14 Nov 1995 08:17:45 -0600
Subject: Re: Edington TEM Monographs

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The Edington series is now distributed by Techbooks in Fairfax (Herndon?), VA.
They have combined the four (five?) books into one volume which sells for about
$55. The original (Philips) series has been out of print for some time now. I
am not sure that the images in the Techbooks version are as good as in the
original, but the books are still an excellent resource. Their phone number is
(703) 352-0001.

Dick Fonda


krogers-at-ecn.purdue.edu (Kirk Rogers) wrote
}
} Does Anyone know where I can get a copy of the "Monographs in Practical
} Electron Microscopy in Materials Science" parts 1-4, by J. W. Edington?
} They were origonally published by the Philips Technical Library/MacMillan,
} but I heard the series was out of print.
} Any suggestions would be helpful.
}
} Thanks,
} -Kirk
} ________________________________________________
} Kirk A. Rogers
} krogers-at-materials.ecn.purdue.edu
} 317-494-8751 office http://materials.ecn.purdue.edu/~krogers
} 317-494-1204 FAX
} Purdue University, School of Materials Engineering,
} 1289 MSEE building, W. Lafayette, IN 47907-1289



_____________________________________________________________
Richard W. Fonda Naval Research Laboratory
(202) 767-2622 Code 6324
_____________________________________________________________





From: tphillips-at-biosci.mbp.missouri.edu (Tom Phillips)
Date: Tue, 14 Nov 1995 09:17:03 -0500
Subject: mounting media for methyl salicylate preps

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Message-Id: {v01510103acce54cb635e-at-[128.206.15.189]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

One of my colleagues has some fluorescent wholemount and sections of
tissues that were dehydrated and cleared in methyl salicylate. Does anyone
have a good suggestion for a permanent mounting medium? Thanks in advance.



Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility
3 Tucker Hall
University of Missouri
Columbia, MO 65211
(314)-882-4712 (voice)
(314)-882-0123 (fax)






From: colijn-at-kcgl1.eng.ohio-state.edu (Henk Colijn)
Date: Tue, 14 Nov 1995 09:03:01 -0500 (EST)
Subject: Re: Edington TEM Monographs

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You can get the book from

Tech Books, Inc,
2600 Seskey Glen Ct.
Herndon, VA 22071
(703) 758-1518

The price is $55. It is a fair quality single volume reproduction printed
in India. Philips told me a number of years ago that they had lost the
masters, so I think that this is about the best quality you can get.

Cheers, Henk


}
} Does Anyone know where I can get a copy of the "Monographs in Practical
} Electron Microscopy in Materials Science" parts 1-4, by J. W. Edington?
}
} They were origonally published by the Philips Technical Library/MacMillan,
} but I heard the series was out of print.
}
} Any suggestions would be helpful.
}
} Thanks,
}
}
} -Kirk
} ________________________________________________
} Kirk A. Rogers
} krogers-at-materials.ecn.purdue.edu
} 317-494-8751 office http://materials.ecn.purdue.edu/~krogers
} 317-494-1204 FAX
} Purdue University, School of Materials Engineering,
} 1289 MSEE building, W. Lafayette, IN 47907-1289

Henk Colijn colijn.1-at-osu.edu OSU Campus Electron Optics Facility
-------------------------------------------------------------------
An optimist believes that we live in the best of all possible worlds.
A pessimist fears this is true.






From: colijn-at-kcgl1.eng.ohio-state.edu (Henk Colijn)
Date: Tue, 14 Nov 1995 09:03:01 -0500 (EST)
Subject: Re: Edington TEM Monographs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You can get the book from

Tech Books, Inc,
2600 Seskey Glen Ct.
Herndon, VA 22071
(703) 758-1518

The price is $55. It is a fair quality single volume reproduction printed
in India. Philips told me a number of years ago that they had lost the
masters, so I think that this is about the best quality you can get.

Cheers, Henk


}
} Does Anyone know where I can get a copy of the "Monographs in Practical
} Electron Microscopy in Materials Science" parts 1-4, by J. W. Edington?
}
} They were origonally published by the Philips Technical Library/MacMillan,
} but I heard the series was out of print.
}
} Any suggestions would be helpful.
}
} Thanks,
}
}
} -Kirk
} ________________________________________________
} Kirk A. Rogers
} krogers-at-materials.ecn.purdue.edu
} 317-494-8751 office http://materials.ecn.purdue.edu/~krogers
} 317-494-1204 FAX
} Purdue University, School of Materials Engineering,
} 1289 MSEE building, W. Lafayette, IN 47907-1289

Henk Colijn colijn.1-at-osu.edu OSU Campus Electron Optics Facility
-------------------------------------------------------------------
An optimist believes that we live in the best of all possible worlds.
A pessimist fears this is true.






From: delannoy-at-welchlink.welch.jhu.edu (Michael Delannoy)
Date: Tue, 14 Nov 1995 12:54:58 -0500
Subject: Used PXL wanted

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Hello to all,
I was wondering if anyone out there has a used
photometrics PXL cooled CCD (preferably with the kodak
1400 chip), that they would be willing to part with.
Please contact me at the above address or call, and we can
discuss terms. Thank you.

Michael Delannoy
Microscopy Facility
JHMI
Baltimore, Md.
(410) 955-1365





From: JANET SUE FOLMER :      jfol-at-welchlink.welch.jhu.edu
Date: Tue, 14 Nov 1995 14:54:57 -0500 (EST)
Subject: please subscribe

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please subscribe






From: Louisa.Howard-at-Dartmouth.EDU (Louisa Howard)
Date: 14 Nov 95 14:36:06 EST
Subject: immuno/Au labelling e-coli

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Message-id: {6949942-at-prancer.Dartmouth.EDU}

A lab in our Biochemistry department is interested in labelling a membrane
protein, found in the inner membrane of e-coli. At the present time, I don't
have a set-up for embedding in Lowicryl. So has anyone used LR white for thin
section immunoAu labelling of proteins found in e-coli? Or, has anyone used
Nanogold/ silver enhancement to label proteins in e-coli, before embedding. We
are experts in TEM and a variety of labelling techniques, but novices with
e-coli; so specific references or preparation methods would be greatly
appreciated.

Louisa Howard
EM Facility/ Remsen 240
Dartmouth Medical School
Hanover, NH. 03755





From: Fermin, Cesar :      fermin-at-TMC.Tulane.Edu
Date: 14 Nov 1995 12:54:34 -0500
Subject: Laser vs. digital deconvolution

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Message-Id: {199511141857.NAA23038-at-thomas.ge.com}
X-Authentication-Warning: thomas.ge.com: daemon owned process doing -bs


Ref.: Laser vs. digital deconvolution

I am not associated with the company that makes the product and gives this
information as a user's advice.

You may want to check VayTek, inc. (514 472-2227) headed by John Kesterson,
Ph.D. Their Micro-Tome deconvolution softwares costs 10-20K and are good.
Thus, if you already have a good microscopic setup..... It is possible that
VayTEk goes into partnership with ONCOR (1-800 77-ONCOR), but I am not sure on
details.
I will be interested on the outcome of the results, and perhaps you can
compile relevant messages and make them available because I too may purchase
a digital decovulating package, only thing I need to add confocal to my lab.

******************************************************************
*Cesar D. Fermin, Ph.D \|T|/ Fax (504) 587-7389 *
*Tulane Medical School /|M|\ Voice Mail (504) 584-2618 *
*Pathology/SL79 \|C|/ Secretary (504) 584-2436 *
*New Orleans La 70112-2699 /|*|\ Lab/Techn. (504) 584-2521 *
* {Fermin-at-tmc.tulane.edu} Dir. Morphological Services *
******************************************************************




From: !Microscopy-request-at-Sparc5.Microscopy.Com (Jean Leclef)
Date: Fri Oct 27 13:37:23 +0100 1995
Subject: Laser printing

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Sender: kayton-at-ohsu.edu (Robert Kayton)
Message-Version: 2
} To: microscopy-at-Sparc5.Microscopy.Com


for those who are intested in laser printing

the following company in New York has a very good alternative to print
images on Laserjet 3/4/4+
printers - it is a German interface board with 150, 300 and even 600 dpi
printing capability, with a palette of 256 from 1024 gray levels. it works
really fine for printing hires SEM images and prints a 2 MByte image in seconds!

the experience shows that it is a real alternative to photographic images
when you have to find another way to print good pictures from a SEM/STEM.

contact :
Electro Image, Inc.
9, Round Hill Road
LAKE SUCCESS, NEW YORK 11020
tel 516 773 4305
fax 516 773 2955


Jean Louis Leclef





From: oshel-at-ux1.cso.uiuc.edu (Philip Oshel)
Date: Tue, 14 Nov 1995 20:32:53 -0500
Subject: Re: mounting media for methyl salicylate preps

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Message-Id: {v02120d02accef070d1b7-at-[128.174.23.95]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

} One of my colleagues has some fluorescent wholemount and sections of
} tissues that were dehydrated and cleared in methyl salicylate. Does anyone
} have a good suggestion for a permanent mounting medium? Thanks in advance.
}
}
}
} Thomas E. Phillips, Ph.D.

I have successfully used methlysalicylate--the final clearing
solution--for long-term storage of fish cleared this way. You might also
try transferring them to 100% glycerin, probably through a graded series,
but I have not tried that and don't know how well it would work.

&&& Illigitimi non carborundum &&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&
Philip Oshel
Center for Electron Microscopy
University of Illinois
(217)244-3145
oshel-at-ux1.cso.uiuc.edu






From: OptoMech-at-aol.com
Date: Tue, 14 Nov 1995 22:06:11 -0500
Subject: TIRFM?

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Does anyone know where I can get some reading material on Total Internal
Reflectance Fluorescence Microscopy?

TGG




From: !Microscopy-request-at-Sparc5.Microscopy.Com (Albert Romano Rodriguez)
Date: Fri Oct 27 08:49:26 +0100 95
Subject: Microscopy Manual Online

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Sender: kayton-at-ohsu.edu (Robert Kayton)
Message-Version: 2
} To: microscopy-at-Sparc5.Microscopy.Com

Dear Fellow microscopists,

Probably the question I'm going to ask you has already
been answered several times, but up to now we did not bother
about it, so that I surely deleted all related mails that have
probably been sent.

In our laboratory we are thinking about changing the
procedure for printing our TEM negatives (although we do some
minor work with the SEM). Up to now we use the standard
printing on paper, but as the amount of pictures we are generating
is always increasing, we think that we should change and start
with the procedure of digitazing (the negatives) and printing them.
We are thinking about purchasing a flat scanner to scan
the negatives and a high resolution printer (perhaps an ink
sublimation printer).
We would appreciate receiving comments on this subject and
to hear the experience of other laboratories: which scanners are
OK or which should be the minimum resolution of them, which printer
type and resolution, computer and physical support to keep the
images (writeable) CD, ..., need for an image treatment software,
aso.

Thank you in advance,

Albert Romano-Rodriguez

EME, Electronic Materials and Engineering
Dept. Applied Physics and Electronics
University of Barcelona
Diagonal 645-647
E-08028 BARCELONA
Spain
e-mail: romano-at-iris1.fae.ub.es
tel: +34 3 402 11 47
FAX: +34 3 402 11 48





From: !Microscopy-request-at-Sparc5.Microscopy.Com (Robert Kayton,MAC,CROET)
Date: Fri Oct 27 15:52:22 -0400 1995
Subject: More on Lexmark Optra R

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Sender: kayton-at-ohsu.edu (Robert Kayton)
Message-Version: 2
} To: microscopy-at-Sparc5.Microscopy.Com

In response to a number of questions received on the Lexmark Optra R:
1) Printing speed is 16 ppm at 600 dpi and 8 ppm at 1200 dpi and uses a
32-bit RISC processor.
2) To print a full 8.5" x 11" page of text, takes about 20 seconds from
"go".
3) Toner comes in two packages - one for approx. 7000 pages is $179.95 and
the other, for approx. 14000 pages is $265.95. These prices are out of the
Misco catelog and one may be able to do better.
4) I previously mentioned the option of providing additional Printer Memory
(I called it "RAM"). One can also purchase a Flash Memory Option to store
information like downloaded fonts and macros.
5) The printer has two connections on the system board that can be used for
either a Disk Option or a Network Option (or two Network Options, or one of
each).
Disk Option: A 40MB hard disk and a thumbscrew - for example, for
downloaded fonts and macros.
Network Option: To connect directly to a LAN - Token-Ring, Ethernet
(10BASE2 or 10 BASE-T) or LocalTalk (whatever that is?). This option
consists of a network card, a thumbscrew, a diskette containing the Network
Printer Utility and documentation. You will need to provide the appropriate
network cable.
6) I do not know the prices of any of the options but you can call
(800)358-5835 for the name of a dealer near you.
7) In case of a problem, they will express an exchange printer OR repair and
return yours. Apparently there is an on-site warranty repair service which I
do not have. IBM, however, can provide local service.
Lastly - in my previous note I may not have advised how REALLY easy this
printer is to operate. I have had mine for a month now - and have not
refered to the manual even once! Even in start-up, the printer "looked at"
my computer software and adjusted itself to my previous printer driver.
Prior to purchasing this unit, I looked at many high end laser printers and
could not find any, at any price, that would even start to compare.
I welcome any other inquiries,
Don Grimes, Microscopy Today





From: !Microscopy-request-at-Sparc5.Microscopy.Com (Robert Kayton,MAC,CROET)
Date: Mon Oct 30 16:46:44 -0500 1995
Subject: Re: Microwave fixation

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Sender: kayton-at-ohsu.edu (Robert Kayton)
Message-Version: 2
} To: MICROSCOPY-at-Sparc5.Microscopy.Com

1) Rapid (i.e.,in seconds) microwave fixation is used with success to fix plant
and animal tissues. In addition, several reports show the benefits of using
microwave fixation to preserve soft tissues encased by hard shells (e.g., clams,
teeth, insects).

Recommended reading:
Login, G. R., & Dvorak, A. M. (1994). Application of microwave fixation
techniques in pathology to neuroscience studies: A review. J Neurosci
Methods, 55, 173-182.
Login, G. R., & Dvorak, A. M. (1994). Methods of microwave fixation for
microscopy. A review of research and clinical applications: 1970-1992. Prog
Histochem Cytochem, 27/4, 1-127.
Kok, L. P., & Boon, M. E. (1992). Microwave Cookbook for Microscopists. (3rd
ed.). Leyden: Coulomb Press.


2) Microwave curing of resins (acrylics and epon substitutes) is also
successful.
Recommended reading:
Giammara, B. (1993). Microwave embedment for light and electron microscopy
using Epoxy resins, LR White, and other polymers. Scanning, 15, 82-87.
Login, G. R., & Dvorak, A. M. (1994). The Microwave Toolbook. A Practical
Guide for Microscopists. Boston: Beth Israel Hospital.


3) Microwave processing is used by some large labs for rapid throughput of
specimens. To date, no automatic microwave processors exist so- although
microwave processing is rapid it is also a bit labor intensive.
Recommended reading:
Leong, A. S.-Y. (1991). Microwave fixation and rapid processing in a large
throughput histopathology laboratory. Pathol, 23, 271-273.
Leong, A. S.-Y. (1993). Microwave techniques for diagnostic laboratories.
Scanning, 15, 88-98.


4) The major problems reported with microwave methods are lack of uniformity and
reproduciblity. ALL microwave ovens have high and low energy areas. The
literature reports several methods for calibrating microwave ovens to improve
results.

Login, G. R., & Dvorak, A. M. (1994). The Microwave Toolbook. A Practical
Guide for Microscopists. Boston: Beth Israel Hospital.
Giberson, R. T., & Demaree, R. S., Jr (1995). Microwave fixation: understanding
the variables to achieve rapid reproducible results. Microsc Res Tech, 32,
246-254.
Gu, J., Forte, M., Hance, H., Carson, N., Xenachis, C., & Rufner, R. (1994).
Microwave fixation, antigen retrieval and accelerated immunocytochemistry.
Cell Vision, 1(1), 76-77.

5) Fortunately, many companies which sell Microscopy products have also
designed, tested, and now sell microwave devices and accessories which improve
microwave methods and most importantly make them safer to use in the laboratory.


Please contact me if you have additional questions.

Gary R. Login, D.M.D., D.M.Sc.
Assistant Professor of Oral Pathology
Beth Israel Hospital
Department of Pathology
330 Brookline Avenue
Boston, Massachusetts, 02215

glogin-at- bih.harvard.edu
Telephone: 617-667-2034
Fax: 617-667-8676





From: ScottE57-at-aol.com
Date: Tue, 14 Nov 1995 22:09:05 -0500
Subject: Re: LM/ Hg HBO 50W lifetimes

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I have previously worked for Zeiss for over 10 years in microscope sales - in
that time I have found that the 100 DC HBO burners would run for 200 to 600
hours with the only problem of light output diminishing and problems then
getting even illumination over the 175 to 225 hour mark. The 50W HBO AC is a
different story - the buld is rated at 100 hours but depending upon operating
conditions they will cloud up and darken as fast as 60-75 hours of use and I
have had in 15 years in the indusrty 4 blow up violently - they do not go
quitely like the 100 DC models. All four were in the 75 to 150 hour range.
As such I have highly encouraged people to change at 100 hours!! - the Bulbs
are same price but upon intial purchase the DC power suplly is considerably
more expensive - you pay up front anf get long and safe bulb life or you save
now and pay in the number of bulbs purchased. Depending upon manufacture the
difference could be $300 for AC aupply vs. $2500 for DC supply. $2000 buys
10 to 15 bulbs and if you are a heavy fluoresence user than get the 100 if
not the 50 is a good way to put more money into the optics where it belongs.

Scott E. Berman
Advanced Imaging Concepts
Princeton, NJ




From: Dave Phelan :      EMU-at-medicine.newcastle.edu.au
Date: Wed, 15 Nov 1995 14:49:39 GMT +11
Subject: C fibre for Balzers coater

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G'day

Does anyone know of a supplier (Australian?) of carbon fibre for a
Balzers model CED 010 carbon coating unit. The fibre supplied by
Balzers has recently increased in price to A$95 for 3 metres. They
have also changed their supplier and the new thinner thread gives a
thinner coating in our system.

Thanks for any suggestions,

Dave


------------------------------------------------------------------
Dave Phelan
Electron Microscope Unit Ph (049) 215667 [+61 49 215667]
University of Newcastle Fax (049) 215669 [+61 49 215669]
NSW 2308 Email emu-at-medicine.newcastle.edu.au
AUSTRALIA
------------------------------------------------------------------





From: Liang, Long :      LLIANG-at-is.arco.com
Date: 15 Nov 1995 10:45:10 CST
Subject: SEM--Phase connectivity ?

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X-SMTP-Posting-Origin: mailhost.pharma.mcgill.ca (Mailhost.Pharma.McGill.CA [132.206.220.7])


Dear microscopists,

Does anyone know of a software which I can use to estimate the
connectivity of phases of interest ? I have some SEM binary images
showing pore distribution patterns, and would like to determine how well
the pores connected to each other, based on their connectivity values.

Thanks.

Long Liang
ARCO EPMA/SEM Lab
Plano, TX
lliang-at-is.arco.com






From: LUCY RU-SIU YIN :      lyin-at-oitunix.oit.umass.edu
Date: Wed, 15 Nov 1995 12:47:09 -0500 (EST)
Subject: listserver

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Hi, this is Lucy Yin from microscopy center at Amherst, Univ. of Mass. I
would like to be on the listserver of MSA microscopy. Please tell me the
procedure to get on . Thanks, Lucy 95-11-15




From: krogers-at-ecn.purdue.edu (Kirk Rogers)
Date: Wed, 15 Nov 1995 11:40:02 -0500
Subject: No Longer a Hoax - Good Times Virus

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Message-Id: {v01530500accfc6e34206-at-[128.46.155.231]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Microscopy Friends and others -

According to the 15 November edition of the Wall Street Journal (pg B8) the
good times virus is no longer a hoax. The newest version of the e-mail has
an attached file named either "install.exe" or "AOL Gold" which, when
installed may render the hard drive inoperative according to an AOL
spokeswoman. AOL plans on notifying all its members today.


-Kirk
________________________________________________
Kirk A. Rogers
krogers-at-materials.ecn.purdue.edu
317-494-8751 office http://materials.ecn.purdue.edu/~krogers
317-494-1204 FAX
Purdue University, School of Materials Engineering,
1289 MSEE building, W. Lafayette, IN 47907-1289






From: sbarlow-at-sunstroke.sdsu.edu (Steve Barlow)
Date: Wed, 15 Nov 1995 11:01:10 -0800 (PST)
Subject: EM:freeze substitution

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I'm looking for researchers using freeze substitution in their SEM and TEM
protocols. I know there are several nice insturments for this
protocol--I'ld like to contact researchers who are doing biological freeze
substitution without the benefit of these expensive devices and who reside
in the southwestern USA

I'ld appreciate any help in this regard

thanks in advance

steve barlow


---------------------------------------------------------------------------
------------------------------------------------------------------------------
Dr. Steve Barlow
EM Facility/Biology Dept.
San Diego State University
San Diego CA 92182-4614
phone: (619) 594-4523
fax: (619) 594-5676
email: sbarlow-at-sunstroke.sdsu.edu





From: dshubito-at-d.imap.itd.umich.edu (Dennis Shubitowski)
Date: Wed, 15 Nov 1995 16:52:31 -0500
Subject: Sodium Lamp - Thanks

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Message-Id: {v02120d01acd011752135-at-[141.211.157.61]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Thank you for all the replies regarding replacement sources
for the sodium vapor lamp bulb. National Graphic Supply
did indeed carry them for $85 which seemed to be the best
price around. I will also look into sodium lamps from
home improvement stores which seems like a viable
alternative.

Thanks again for all the suggestions!

Sincerely,
Dennis Shubitowski
University of Michigan
School of Dentistry
dshubito-at-umich.edu






From: Microbill-at-aol.com
Date: Wed, 15 Nov 1995 17:52:36 -0500
Subject: Re: Posting to the Microscopy...

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Nestor - it's not easy getting old - sorry for the typo and thanks.

Bill Miller




From: Michael Shaffer :      mshaf-at-darkwing.uoregon.edu
Date: Wed, 15 Nov 1995 08:38:39 -0800 (PST)
Subject: Re:

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At , you wrote:
} Dear Fellow microscopists,
}
[ ... snip ... ]

} We are thinking about purchasing a flat scanner to scan
} the negatives and a high resolution printer (perhaps an ink
} sublimation printer).

} We would appreciate receiving comments on this subject and
} to hear the experience of other laboratories: which scanners are
} OK or which should be the minimum resolution of them, which printer
} type and resolution, computer and physical support to keep the
} images (writeable) CD, ..., need for an image treatment software,
} also.
}
Thank you in advance,
}
} Albert,
}
We use the HP IIcx flatbed which is no longer available, but is now sold
as a HP 3c ( {$1000). It is a very capable scanner, but you have to buy the
transparency adapter for an extra $600. As a WIntel applet the twain
software is very solid but they haven't delivered a 32bit driver yet for
Win95/NT ... the 16bit still works however.

Regarding dye-sub printers ... I suppose you realize they are color
printers and you pay $extra$ to print on color stock ( {$3/pg) in spite of
the image being monochrome. The option does exist for grayscale ribbons
( {$2/pg), but they are more than a hassle if you use color sometimes and
want to use grayscale at times. We ^do^ use a Codonics ethernet printer
which has the Kodak dye-sub engine ... and we can reccommend it ... it is a
different type of ethernet printer ... let me know if you want to know more
about it. As another option there are Ag salt laser printers available which
print grayscale at 256dpi which are ^not^ dithered ... ie, ^true^ 256 shades
of gray. They are expensive ( {$14k), but the cost is less than a $1/pg.

Regarding, archival storage of image files ... you should $invest$ in
magneto-optical. We chose to go with a Fujitsu 230MO drive ( {$700) for which
the replacable media costs {$30 and can be found for near $20. The other
option which we now wished we could have afforded would have been to go with
1.3Gb drives for which the drives are more expensive, but the media costs
are less expensive (per Mb). These MO drives offer the freedom of
write/delete ..., a third option would be write once CR-ROM drives for
archiving ... again the drives are expensive but the media is less than $15
for 600Mb. Lastly, in this regard and to mention alternatives, eg Zip
drives, some people would imply they shouldn't be used for "archiving" as
the magnetic media shouldn't be trusted to any degree more than floppies or
hard drives for occasional and intangible influences. On the other hand, MO
drives require an annealing temperture to change a bit, which also means
they write more slowly but they do read quickly.

Hope this helps ..

cheers, shaf


{\/} /\ {\/} /\ {\/} /\ {\/} cognito, ergo zZOooOM {\/} /\ {\/} /\ {\/} /\ {\/}
Michael Shaffer, R.A. - University of Oregon Electron Probe Facility
mshaf-at-oregon.uoregon.edu -or- mshaf-at-darkwing.uoregon.edu
http://darkwing.uoregon.edu/~mshaf/epmahome/





From: Donna Wagahoff :      DWAGAHOF-at-wpsmtp.siumed.edu
Date: Wed, 15 Nov 1995 17:29:54 -0600
Subject: used TEM available

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Message-Id: {s0aa22e3.045-at-wpsmtp.siumed.edu}
X-Mailer: Novell GroupWise 4.1

** Reply Requested by 11/16/1995 (Thursday) **

We have a Philips 201C TEM that we must get rid of to free-up some
space in our lab. If you are interested, please contact me and we can
discuss the details of its availability. Thanks!
Donna Wagahoff
SIU School of Medicine
P.O. Box 19230
Springfield, Il. 62794-1220
phone 217-782-0898
fax 217-524-3227





From: dac-at-bio.umass.edu
Date: Wed, 15 Nov 1995 12:21:39 -0500 (EST)
Subject: Re: Arc Lamps

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****************************************************************
I am sending this message to the list as a whole since I mistakenly
used that forbidden REPLY button and I think it went to only one
recipient and I had hoped that it would be more generally informative.
****************************************************************

The question was about using arc lamps for more than the rated life.
The characteristics of arc lamps are the key to understanding what may
be reasonably done with them, but the wide variey of power supplies
that are available are the other part of that equation and often little
information is available to help users in this regard. Armed with the
detailed characteristics of mercury arc lamps, please go after your
manual or vendor for the missing part so that you may make the right
decisions.

Mercury arc lamps are given a nominal lifetime rating based on 30 min
of operation per start and operation at the specified conditions. Each
ignition of a mercury arc does some damage to the electrodes. A
sufficient current at a high voltage is required to break over the arc
in the cold lamp where the mercury is not in the high pressure vapor
state. This energy serves to vaporize some mercury and get the arc
established, at which point it can be maintained at a lower current and
voltage. This large surge erodes the tips of the electrodes, opening
the spacing a little each time. Both in starting and in operation, the
electrode spacing is involved in establishing the arc voltage at the
operating current. When the spacing is too large, ignition and
operation become more difficult as described below. The ignition
circuitry that Osram, for example, describes in their literature of a
few years back, is simple passive electronics that simply do the job.
More modern electronic units CAN start the lamps more gently and this,
together with longer operation per start, will be reflected in longer
usable lamp life.

Mercury arc lamps have nominal ratings: a 100W lamp operated at the
nominal 5 amps current will only give 100 watts at one brief part of
its cycle. This is because of the change of the arc electrode spacing
(and thus arc voltage). Most early power supplies, whether DC or AC
used a choke to limit and stabilize the lamp current; since the arc
voltage is much lower than the line voltage, this gave an approximation
of constant current operation. I have found that a new HBO100W/2 lamp
warmed-up and operated at 5 amps with a good DC supply has an arc
voltage of about 14.8V, giving only about 75W. At 200 hours of life,
this lamp had 28V across the arc (-at- 5 amps) and this is 140W! No doubt
this relationship would continue until a) the lamp gets too hot and
explodes, b) the power supply operating voltage becomes insufficient to
maintain 5A current (giving a decrease in power and dimming of lamp
output), or c) the power supply is unable to ignite and stabilize the
lamp at the higher arc voltage. It is possible to operate the HBO100W/2
lamp at currents as low as 1 amp with the right equipment, although the
light output is also way down. If you have a proper power supply and
the light output is adequate, operating at less than the nominal rating
should give a real extension of lamp life.

Another issue is the operating temperature of the lamp. Osram specifies
that the lamp bases should be no higher than 230C degrees and that it
SHOULD be operated at below 200C if possible. Lamp housings should be
designed to provide sufficient heat dissipation at the nominal wattage
by passive heat flow or air flow directed at the lamp bases: the quartz
envelope must be hot to have stable Hg temperature and pressure but an
excessive gradient along the lamp will produce excessive stress on the
lamp. Clearly, with economy power supplies the power dissipation
cannot be limited and at some point the lamp will get too hot and you
risk catastrophic failure. More expensive electronic supplies allow for
various sorts of automatic control or manual readout and adjustments to
control or limit power levels.

In summary, there are very good reasons that modern equipment can give
some lengthening of lamp life, but there are very real reasons not to
push this too far: the lamps simply DO get consumed in the process of
giving light. I have personally seen a 2" quartz collector shattered by
a lamp explosion and that is quite an expense to replace.


Sincerely,

Dale A. Callaham
Central Microscopy Facility
University of Massachusetts
Amherst, MA 01003 USA

email: dac-at-bio.umass.edu





From: Greg :      GREG-at-umic.umic.sunysb.edu
Date: Wed, 15 Nov 1995 13:05:00 EST5DST
Subject: Re: sample charging

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} Date: Tue, 14 Nov 95 13:58:47 EST
} From: "MARK DARUS, 8*346-2895, (216)266-2895" {darus-at-cle.dnet.ge.com}
} X-To: mail11:; (-at-micro)
} To: microscopy-at-sparc5.microscopy.com
} Subject: sample charging

Mark,
I assume that you are using an SEM and not a TEM.
You did not say if you have tried using backscatter
imaging to view your sample. It is not as effected by
charging as secondary imaging and it will still give a good
image.
You can also try making a carbon paint trail from the stub
to the top of the sample. This can make it easier for the
electrons to get out of the sample.

Greg-at-umic.umic.sunysb.edu
S.U.N.Y. at Stony Brook
University Microscopy Imaging Center
}
} Does anyone have a "best" method for analyzing sections of
}
} quartz tubing, without it charging? I carbon coated the sample 3 times,
}
} I lowered the Kv, increased the strength of the condenser lens, increased
}
} the bias, increased the scan rate and tilted the stage 35-45 degrees.
}
} Still I get a lot of charging. When I can manage to get an area that is not
}
} charging, and am able to focus on it, my counts are very low, for EDX. The
}
} samples are sections of quartz tubing, that have either inclusions or
}
} devitrification that is tinted. Should I just continue adjusting the above
}
} parameters, or is there something else that I should try?
}
}
} Thanks
}
} Mark Darus
}
} Darus-at-cle.dnet.ge.com
}





From: Dr. Andrew P. Somlyo :      aps2n-at-elvis.med.virginia.edu
Date: Wed, 15 Nov 1995 15:42:10 -0500 (EST)
Subject: Re: EDS system evaluation

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Message-ID: {n1395689354.57709-at-ms.sjdccd.cc.ca.us}

There actually is a manual in microscopy that gets updated quarterly. It is
put out by John Wiley and is called Procedures in Microscopy. It comes in a 3
ring binder so it can be easily updated. It is similar to the one in
Molecular Biology if you are familiar with that. It has been out at least 2
years and IS updated regularly.
Cheers,
Judy M

Judy Murphy, PhD
Dept. of Microscopy
San Joaquin Delta College
5151 Pacific Ave
Stockton, CA 95207

Phone: 209/474-5284
FAX: 209/474-5649
e-mail: murphy.ms.sjdccd.cc.ca.us

_______________________________________________________________________________

It seems to me that a manual of microscopy techniques would be
extremely useful, better in several ways than a book. I've set out
my thoughts on this in the following URLs (they contain identical
information but access speed will be better from your closest site).

Europe - {URL:http://www.lars.bbsrc.ac.uk/micro/in-focus/9511.html}
America - {URL:http://metro.turnpike.net/jefferie/in-focus/9511.html}

If the idea interests you, please take a look and let me have some
comments. If there's enough response perhaps we could get a group of
people discussing it more formally by e-mail to hammer out some
consensus views.

Thanks,

Chris
--
-----------------------------------------------------------------------------
| Chris Jefferies E-Mail (home) - chris-at-stowey.demon.co.uk |
| (work) - chris.jefferies-at-bbsrc.ac.uk |
| Microscopy page (UK) {URL: http://www.lars.bbsrc.ac.uk/micro/} |
| (USA) {URL: http://metro.turnpike.net/jefferie/} |
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We have an ISIS system and I would also like to hear whether anyone has
successfully implemented Bioquant and, if so, what standards were used.

On Fri, 10 Nov 1995, Fiona Graham wrote:

} We are in the market for a new energy dispersive x-ray analysis system
} to attach to a Hitachi S520 SEM. The systems we are considering
} include:
}
} LINK ISIS
} NORAN 3050
} EDAX DX4
} IRIDIUM II microanalysis system.
}
} If any current users of these systems have any comments about the
} systems, we would most appreciate hearing from you.
}
} Thanks in advance
} Dr Fiona Graham
} EM Unit
} University of Natal, Durban
} South Africa
} email: graham-at-ph.und.ac.za
} tel: +27 31 2602174 fax: +27 31 2616550
}




From: sbarlow-at-sunstroke.sdsu.edu (Steve Barlow)
Date: Wed, 15 Nov 1995 10:21:02 -0800 (PST)
Subject: EM:Workshop on microwave fixation

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MICROWAVE WORKSHOP 1996
WITH PRIMARY EMPHASIS ON 3-HOUR PROCESSING OF TISSUE FOR TRANSMISSION
ELECTRON MICROSCOPY

SAN DIEGO STATE UNIVERSITY, SAN DIEGO CA JAN 25-27, 1996
Contact Steve Barlow Phone: (619) 594-4523
FAX: (619) 594-5676
email: sbarlow-at-sunstroke.sdsu.edu

SEE BELOW FOR ALTERNATIVE DATES/SITES

Routine processing of tissue for transmission electron microscopy in
clinical and research applications is a time consuming process. This
workshop focuses on the use of a laboratory microwave oven as a means to
accelerate all aspects of tissue processing for TEM. The demonstrations,
course handouts and "hands-on" experience will provide workshop
participants with the tools necessary to rapidly assimilate a laboratory
microwave into their protocols. Other areas covered in the workshop
include microwave demonstrations of histological staining (periodic
acid-methenamine silver stain of kidney basement membranes), immunostaining
for TEM and digital image acquisition and printing. Each workshop is an
intensive two and a half-day experience based on the following:

Giberson, R.T., Demaree, R.S., Jr. (1995) Microwave fixation:
Understanding the variables to achieve rapid reproducible results. Micros.
Res. & Tech (in press)

Giberson, R.T., Smith, R.L., Demaree, R.S. (1995) Three hour microwave
tissue processing for transmission electron microscopy: From unfixed tissue
to sections. Scanning 17, Suppl. V:26-27

Demaree, R.S., Jr., Giberson, R.T., Smith, R.L. (1995) Routine microwave
polymerization of resins for transmission electron microscopy. Scanning
17, Suppl.
V:25-26

"Hands-on" Microwave Workshop for TEM and Histology
Calif. State University, Chico, June 15-17, 1995



COURSE OUTLINE, SAN DIEGO STATE UNIVERSITY

Jan 25, 1996 8:30-5:00 Lecture and microwave demonstration by R.
T. Giberson

Demonstration of 3 hour microwave tissue processing

Jan 26, 1996 8:00-5:00 Participants will work in groups of 5 and
process tissue using 3

hour protocol in laboratory microwaves

Jan 27, 1996 8:00-12:00 Microwave demonstrations of on-grid
immunostaining, histological

staining, and digital imaging

Registration Fee for the SAN DIEGO WORKSHOP (workshop limited to 20
participants)

Workshop ONLY (includes the following) $500.00

1. Group dinner at the end of the first day
2. Lunch the first two days
3. Course handouts and all materials used in the workshop
4. Certificates of Completion mailed to each participant

Workshop AND 3 DAYS LODGING $700
at local hotel for 3 nights starting the night before the
workshop

1. Group dinner at the end of the first day
2. Lunch the first two days
3. Course handouts and all materials used in the workshop
4. Certificates of Completion mailed to each participant

In the event a participant needs to cancel, written notification (fax is
acceptable) is required. A $50.00 administrative fee will be charged for
cancellations. 50% of the registration fee will be reimbursed for
cancellations within 30 days of the workshop. Substitutions are
acceptable.

ALTERNATIVE LOCATIONS AND DATES:

University of Maryland Dept. of Zoology, College Park, MD JAN 3-5, 1996
Contact Tim Maugel- Phone (301) 405-6898
FAX: (301) 314-9358
email: maugel-at-zool.umd.edu

Yale University School of Medicine New Haven CT JAN 10-12, 1996
Contact Paul Webster Phone: (203) 785-3219
FAX: (203) 785-7226
email: paul_webster-at-quickmail.yale.edu

NIH/Rocky Mountain Labs, Hamilton, MT JULY 1-3, 1996
Contact Dave Dorward Phone:(406) 363-9266
FAX: (406) 363-9204
email: dave_dorward-at-nih.gov

Madison Area Technical College, Madison, WI AUG 7-9, 1996
Contact Michael Kostrna Phone:(608) 246-6762
FAX: (608) 246-6880
email: msk5063-at-madison.tec.wi.us


---------------------------------------------------------------------------
------------------------------------------------------------------------------
Dr. Steve Barlow
EM Facility/Biology Dept.
San Diego State University
San Diego CA 92182-4614
phone: (619) 594-4523
fax: (619) 594-5676
email: sbarlow-at-sunstroke.sdsu.edu





From: David.Rothbard-at-ipst.edu (David Rothbard)
Date: Thu, 16 Nov 1995 09:29:41 -0500
Subject: Re: SEM--Phase connectivity ?

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} Does anyone know of a software which I can use to estimate the
} connectivity of phases of interest ? I have some SEM binary images
} showing pore distribution patterns, and would like to determine how well
} the pores connected to each other, based on their connectivity values.

This was done extensively (and published) by Bob Ehrlich using
erosion/dilation. The commercial verison is handled by Perception and
Decision Systems, which can be reached by e-mail at Pads12-at-aol.com. If you
do a literature search of the last 10 years (especially J. Sedimetary
Petrology and AAPG Bull) you may turn up much more. Most generic image
processing packages have erosion/dilation algorithms included.

David Rothbard

--
Institute of Paper Science and Technology






From: Fang He :      fanghe+-at-pitt.edu
Date: Thu, 16 Nov 1995 10:13:45 -0500 (EST)
Subject: unsubscribe

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From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Thu, 16 Nov 1995 09:24:13 -0800
Subject: Measuring pH

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Greetings:

A user in our central EM lab just crunched the electrode on our old analog
pH meter. It was an Orion Ross electrode with a replacement cost of about
$200.

Rather than just replace this expensive electrode on an old meter, I
thought I would ask about what types of pH measurement are in use by other
labs. A recent Fisher catalog has dozens of alternatives. Too many to pick
just one without some advice.

If you have some ideas, let me know. I am looking for the best combination
of cost, accuracy, ease of upkeep, and durability in a multiuser lab.

Jonathan Krupp
Microscopy and Imaging Lab
University of California
Santa Cruz, CA 95064
(408) 459-2477
jmkrupp-at-cats.ucsc.edu






From: bozzola-at-siu.edu (John. J. Bozzola)
Date: Fri, 17 Nov 1995 00:02:54 -0600
Subject: LM: Na Vapor Lamps

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I'm confused regarding the discussion on Na vapor lamps. I believe the
original discussion concerned the use of less expensive (home improvement
store) lamps in darkroom safelights. Did the discussion somehow turn to the
use of such lamps in fluorescent microscopes???? I think I must have missed
a transitional message somewhere. Could some kind soul please update me on
the status. Can cheaper Na vapor lamps be used in both situations. I'd be
(pleasantly) surprized if they could. Many thanks.

#############################################################################
John J. Bozzola, Director
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
#############################################################################






From: M.V. Parthasarathy :      mvp2-at-cornell.edu
Date: Thu, 16 Nov 1995 09:23:06 -0400 (EDT)
Subject: RE: EM:freeze substitution

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In message Wed, 15 Nov 1995 11:01:10 -0800 (PST),
sbarlow-at-sunstroke.sdsu.edu (Steve Barlow) writes:

} I'm looking for researchers using freeze substitution in their SEM and TEM
} protocols. I know there are several nice insturments for this
} protocol--I'ld like to contact researchers who are doing biological
} freeze substitution without the benefit of these expensive devices and
} who reside in the southwestern USA
-----
The recent article on Freeze-substitution (Part I, Chapt.V) on Methods In
Plant Cell Biology, Part A (Edts. Gailbraith et al.; Academic Press, 1995)
might be helpful.

*************************************************************************
M.V. Parthasarathy
Professor of Plant Biology &
Director, Cornell Integrated Microscopy Center
Section of Plant Biology
228 Plant Science Building
Cornell University, Ithaca, NY 14850
Telephone: (607) 255-1734
Fax: (607) 255-5407
E-mail: mvp2-at-cornell.edu
***********************************************************************




From: krogers-at-ecn.purdue.edu (Kirk Rogers)
Date: Thu, 16 Nov 1995 13:19:14 -1812
Subject: Edington Monograph reply summary

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Hello All-

Thank you to all of those who sent me replies regarding "Monographs in
Practical Electron Microscopy in Materials Science" by Edington. The 5
volume set has been combined into 1 volume and is available at a cost of
$55 each. There is a 10% student discount available, and shipping costs
(4.50 for 2 copies) are reasonable, at least in the U.S. There has been
some doubt about the quality of the micrographs in parts, but I believe
that they have to be better than the micrographs in the photocopies I have
currently.

For more information/ To order contact:

Tech Books Inc
4012 Williamsburg Court
Fairfax VA 22032
USA
(800) 767-1518
(703) 352-0001
fax (703) 352 8862

The volume may also be available from

Polycrystal Book Service
P.O. Box 3439
Dayton, OH 45401-3439
USA
telephone and fax: (513) 223-9070

however they did not return my calls.


-Kirk
________________________________________________
Kirk A. Rogers
krogers-at-materials.ecn.purdue.edu
317-494-8751 office http://materials.ecn.purdue.edu/~krogers
317-494-1204 FAX
Purdue University, School of Materials Engineering,
1289 MSEE building, W. Lafayette, IN 47907-1289






From: gwe-at-biotech.ufl.edu (Greg Erdos)
Date: Thu, 16 Nov 1995 09:02:04 GMT
Subject: Re: EM:freeze substitution

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} I'm looking for researchers using freeze substitution in their SEM and TEM
} protocols. I know there are several nice insturments for this
} protocol--I'ld like to contact researchers who are doing biological freeze
} substitution without the benefit of these expensive devices and who reside
} in the southwestern USA
}
} I'ld appreciate any help in this regard
}
} thanks in advance
}
} steve barlow
}
}
} ---------------------------------------------------------------------------
} ------------------------------------------------------------------------------
} Dr. Steve Barlow
} EM Facility/Biology Dept.
} San Diego State University
} San Diego CA 92182-4614
} phone: (619) 594-4523
} fax: (619) 594-5676
} email: sbarlow-at-sunstroke.sdsu.edu
}
} } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } }

The most critical part of freeze substitutuion is the freezing. After that
there are a couple of low tech methods for substitution. :
After freezing the sample cool to liq. nit. temp and place on the
substitution medium that has been solidified by freezing in liq. nit. Then
pack it in dry ice in a styrofoam box and wait for it to come to room temp.

I do the same only I place the samples in a -45 C freezer for two
days then in a - 20C freezer for a day, instaed of on dry ice.

If there Are several freezers available you can place the frozen
sample in - 85 C substitution medium and gradually raise the temp by
moving the sample in the medium from freezer to freezer.
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Greg Erdos Phone: 904-392-1295
Scientific Director,
ICBR Electron Microscopy Core Lab
218 Carr Hall Fax 904-846-0251
University of Florida E-mail: gwe-at-biotech.ufl.edu
Gainesville, FL 32611
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-





From: Paul Webster :      Paul.Webster-at-quickmail.yale.edu
Date: 16 Nov 1995 10:28:32 -0400
Subject: Re: freeze substitution

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Message-Id: {n1395604645.30885-at-QuickMail.Yale.edu}

I don't know if your interest in freeze substitution is to actually try it out
before buying a machine but if it is, here is a neat way to do it.
Fix the biological material (either by freezing or by chemical means).

If you choose chemical fixation you must then cryoprotect the material by
infiltrating with 2.0M sucrose and then freezing on metal pins, by dropping them
in liquid nitrogen.

Once frozen, transfer the material to dry methanol (just open a new bottle)
which is being held in a tube, in a styrofoam box filled with dry ice. Keep the
material in the cold methanol overnight. Remove the methanol and replace with
fresh methanol, leave for a few hours, and replace with a methanol-Lowicryl mix
(1:1). If you want to keep the tubes on dry ice you can use Lowicryl HM 23,
otherwise, transfer the tubes to a freezer. Keep increasing the amount of resin
in the tubes until you are in 100% resin. Leave overnight, replace with fresh
resin and polymerize with UV light. Great embedding (I have pictures) with low
cost.

Variations include adding 1% osmium tetroxide or 1% uranyl acetate to the
methanol. The contrast is subtle but significant and the osmium does not affect
polymerization or immunolabeling.

Paul Webster
Center for Cell Imaging
Yale School of Medicine
http://info.med.yale.edu/cellimg






From: tphillips-at-biosci.mbp.missouri.edu (Tom Phillips)
Date: Thu, 16 Nov 1995 08:03:51 -0500
Subject: RE: Microscopy Manual Online

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Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

But doesn't the Procedures in Microscopy manual cost } $500? Presumably an
online manual maintained by people like us would be free.

} There actually is a manual in microscopy that gets updated quarterly. It is
} put out by John Wiley and is called Procedures in Microscopy. It comes in a 3
} ring binder so it can be easily updated. It is similar to the one in
} Molecular Biology if you are familiar with that. It has been out at least 2
} years and IS updated regularly.
} Cheers,
} Judy M
}
} Judy Murphy, PhD
} Dept. of Microscopy
} San Joaquin Delta College
} 5151 Pacific Ave
} Stockton, CA 95207
}
} Phone: 209/474-5284
} FAX: 209/474-5649
} e-mail: murphy.ms.sjdccd.cc.ca.us
}
} _______________________________________________________________________________
} From: chris-at-stowey.demon.co.uk on Wed, Nov 15, 1995 10:22 AM
} Subject: Microscopy Manual Online
} To: microscopy-at-Sparc5.Microscopy.Com
}
} It seems to me that a manual of microscopy techniques would be
} extremely useful, better in several ways than a book. I've set out
} my thoughts on this in the following URLs (they contain identical
} information but access speed will be better from your closest site).
}
} Europe - {URL:http://www.lars.bbsrc.ac.uk/micro/in-focus/9511.html}
} America - {URL:http://metro.turnpike.net/jefferie/in-focus/9511.html}
}
} If the idea interests you, please take a look and let me have some
} comments. If there's enough response perhaps we could get a group of
} people discussing it more formally by e-mail to hammer out some
} consensus views.
}
} Thanks,
}
} Chris
} --
} -----------------------------------------------------------------------------
} | Chris Jefferies E-Mail (home) - chris-at-stowey.demon.co.uk |
} | (work) - chris.jefferies-at-bbsrc.ac.uk |
} | Microscopy page (UK) {URL: http://www.lars.bbsrc.ac.uk/micro/} |
} | (USA) {URL: http://metro.turnpike.net/jefferie/} |
} -----------------------------------------------------------------------------
}
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Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility
3 Tucker Hall
University of Missouri
Columbia, MO 65211
(314)-882-4712 (voice)
(314)-882-0123 (fax)






From: tvoiles-at-UNLINFO.UNL.EDU
Date: Thu, 16 Nov 1995 15:41:18 -0500
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From: Doug Cromey :      dcromey-at-ccit.arizona.edu
Date: Thu, 16 Nov 1995 10:18:06 -0700
Subject: Microwaves (hardware ?)

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Microscopists,
I am responsable for a small histology service lab. My new histologist has
some experience with microwave stain techniques and use of microwaves to
express antigens in archival-paraffin-embedded material. We are seriously
considering the purchase of a microwave for our lab to speed things up, but
we are wondering about the merits of "off the shelf" microwaves (eg
appliance stores) versus "technical" microwaves available from a number of
reputable microscopy suppliers. There is a considerable price difference
between the two types and I'd like to be educated on the relative merits of
one versus the other (our needs are not likely to include EM processing,
since there is a seperate lab in the Health Sciences Center for that).

Perhaps it would be best if you replied to me personally and I can then post
a summary to the list at a later date.

A note to vendors: I appreciate your input, to save yourself paper &
mailing costs, you might inquire if I already have some of your materials
regarding microwaves before you send me any more.

Thanks,
Doug
.....................................................................
: Douglas W. Cromey, M.S. Dept. of Cell Biology & Anatomy :
: Sr. Research Specialist University of Arizona :
: (office: AHSC 4212A) P.O. Box 245044 :
: (voice: 520-626-2824) Tucson, AZ 85724-5044 USA :
: (FAX: 520-626-2097) (email: dcromey-at-ccit.arizona.edu) :
:...................................................................:





From: deschuyt-at-ccmailg1.sbbio.be (Michel DESCHUYTENEER)
Date: Thu, 16 Nov 1995 14:02:48 +0000
Subject: SEM: nebulizer for particles dispersion

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Fellow microscopists:

I plan to buy a nebulizer to prepare dispersions of particles in the
1-10 micrometers range.
I have seen various models in as many catalogs.
Can anyone recommended a particular model? Is there one to avoid at
all cost?
Thanks in advance for your comments.
MICHEL

Michel Deschuyteneer deschuyt-at-sbbio.be
Scientist - Electron Microscopy Laboratory
SmithKline Beecham Biologicals
Rue de l'Institut, 89 B-1330 Rixensart Belgium
Tel: +32-2-656 9290 Fax: +32-2-656 8113




From: Murphy, Judy :      murphy-at-ms.sjdccd.cc.ca.us
Date: 16 Nov 1995 11:02:49 -0800
Subject: Mohr Processor, HELP

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I have recently been sent a Mohr processor to test to see how it works in our
lab for film and paper as we must get rid of our darkroom. This note is about
the negative processing as I haven't even got to the print processing yet.
Specifically I am having a major problem with Ektapan and SO163 (see below for
specifics)

NOTE: Most of our electron microscopes are analogue so we would like to go
digital but cannot totally do that at this time.

The films we need to have processed are (all Kodak films)
4489 EM film (3 1/4 x 4 inch)
SO163 EM film (twice the speed of 4489) 3 1/4 x 4 inch
Ektapan ( a 4. x 5 sheet film) continuous tone film for making copy negs,
copying pictures, light microscopy in B/W
4127 (4 x 5 sheet film) good continuous tone used for SEM (Our student's can't
afford Polaroid)
The last two films are panchromatic films.

This system allows one to choose the time one processes in each chemical i.e.
Mohr developer and standard Kodak Rapid Fix. This unit has a 600 watt heater
on it. It also has the extended time switch.

We are using a temperature of 88 degrees for the dev and fixer (when measuring
it, the dev is actually 88.5 and the fix is 84.5)

We run several plastic cleaning sheets through the processor before processing
the negatives.

To give an idea of the initial clearing time in Kodak rapid Fix by wet
chemistry, 4489 clears in 20 s in fresh fix; SO163 clears in 37 sec in fresh
fix.

Every session we have worked on the Mohr, we have also developed the negatives
by standard Kodak recommended and tried and proven "wet" chemistry using the
same Kodak fix that is in the processor. All of the "wet chemistry" negatives
have come out fine, as expected.

RESULTS from Mohr
4489
Works fine at the 2 minute setting. dry to dry.

4127. Works fine at 3.5 min. setting.

Ektapan and SO163 are problem childs.
I have tried the Ektapan and SO163 at the following min. settings on the Mohr
machine
2, 2.5, 3, 4, 5, 5.5, 6, 6.5
2 + Extended time (14.5 actual min from dry to dry)
3 + Extended time
6.5 + Extended time (17.5 actual min from dry to dry)
As a rule of thumb, each of the settings on the machine is divided by 4 to get
how much time is actually spent in each place (developer, fix, wash, dry)
Thus the 17.5 actual time is divided by 4, which means 4.4 sec in each place.
The 2 min setting means 30 sec in each place.

Because the first unit I got, the heater was not functionikng, I did my first
tests without a heater. All had to be put in a dryer for about 2 min. to
completely dry which they were. With and without a heater, 4489 and 4127
worked in the same times as indicated above.

Without a heater
Ektapan, 2+Extended Time On (14.5 min. total) appeared to clear the photos,
but wet.
SO163, 5+Extended Time On appeared to clear the photos, but wet.

Heater arrives
4489 and SO163
Did the same tests with all the times, and at all of the times from the
shortest 2.0 (Extended Time OFF) to 6.5+Extended Time ON, it not only didn't
clear it but it precipiated black stuff (which I think is silver) all over the
negatives in a potchy distribution. This could not be cleared anymore in fix
applied outside the Mohr machine or washing outside. It was fried onto the
negs. (Again 4489 worked fine)

I did have fresh fixer and developer. When I ran 4489 in between the Ektapan
and SO163 tests, it still turned out at 2 min. (Ext. Time OFF).

I then removed the wash rack and ran negatives through the Mohr dev and Rapid
fix (in the Mohr machine) and let them drop in the water bath. I did two
things
A. One neg I washed for 30 min. outside the Mohr to be sure it was washed,
then ran it through the wash and dryer roller. The same thing happened.
Stuff precipitated all over the neg. and fried on.
B. One neg I removed after it went through the fix rollers and fixed it
outside, followed by a fix neutralizer and washed it for 20 min outside. I
then ran it through the Mohr wash rollers and dry. It also had stuff on it,
fried to the neg as all of the others, but not quite as bad as those
completely processed in the Mohr. I was very surprised by this results as I
expected that if the unexposed Ag was completely washed out, it shouldn't
happen.

Mohr doesn't have any suggestions and Ted Pella Inc. hasn't heard of this
happening and has no suggestion.
To add to all of this, my darkroom will very shorty be converted into a
room to accept another instrument and I have no way of developing negatives
unless I get a processor to do so. To say the least, my level of frustration
is quite high. I have now spent about 16 hrs. testing this machine in a very
methodical way, and still can't get Ektapan or SO163 to work.
If there are any suggestions or similar experiences, I would really
appreciate them. I really am out of things to try or do.
Thanks
Judy Murphy

Judy Murphy, PhD
Dept. of Microscopy
San Joaquin Delta College
5151 Pacific Ave
Stockton, CA 95207

Phone: 209/474-5284
FAX: 209/474-5649
e-mail: murphy.ms.sjdccd.cc.ca.us









From: Gib Ahlstrand :      giba-at-puccini.crl.umn.edu
Date: Thu, 16 Nov 1995 16:59:39 -0600
Subject: Sample Charging

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Mark, Sometimes (most of the time??) carbon is not an effective coating for
neutralizing charge build-up on samples, biological in particular. Plus you get
a noisy image so image signal quality is not great for photo recording.

Sometimes I've had to coat with metals in order to get a stable sample for doing
x-ray microanalysis. The trick is to pick a metal that won't overlap with any
elemental peaks collected from the sample, and to use as little as possible to
minimize absorption of 0-2.0 KeV x-rays coming from your sample. For example,
I'll use nickel or chromium or aluminum - just a little bit! - and often it will
solve the charging problem so I have the same incident kV of primary electrons,
always an important beam condition to stabilize for x-ray microanalysis.

Good luck,

--

Gib Ahlstrand, MMS Newsletter Editor
Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
495 Borlaug Hall, St. Paul, MN 55108 (612)625-8249
612-625-9728 FAX, giba-at-puccini.crl.umn.edu

"MICROSCOPY & MICROANALYSIS 96" in Minneapolis, Minnesota, Aug.11-15, 1996





From: dwebb-at-waite.adelaide.edu.au (Daryl Webb)
Date: Fri, 17 Nov 1995 12:24:19 +1030 (CST)
Subject: Visual detection of CY-5

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cytometry-at-flowcyt.cyto.purdue.edu (Flow Cytometry)

Hi all, does anyone have experience with CY-5 on a "standard" HBO 50 lamp
based Fl microscope. I've heard that it can be difficult to see (as opposed
to detect with pmt's), the local sales droids have never sold the appropriate
filter block so wont commit themselves. Dont particularly wish to fork out
for the filter block if I wont be able see my results...

Anyone with advise/experience.

ta muchly
--

Daryl Webb (dwebb-at-waite.adelaide.edu.au)
Dept. of Plant Science, Waite Institute
University of Adelaide, Glen Osmond S.A. 5064
Australia. Voice:61_8 303 7426 Fax:61_8 303 7102







From: Michael OKeefe :      Michael_OKeefe-at-macmail.lbl.gov
Date: 16 Nov 1995 12:29:42 U
Subject: FWD>Returned mail- User unk

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Message-Id: {n1395597277.51547-at-macmail.lbl.gov}




From: Jean Leclef
Date: 11/15/95 7:14 PM
Subject: Re: Laser printing

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----- Transcript of session follows -----
Connected to sparc5.microscopy.com:
} } } RCPT To: {!Microscopy-request-at-sparc5.microscopy.com}
{ { { 550 {!Microscopy-request-at-sparc5.microscopy.com} ... User unknown
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Reply to: RE} Laser printing

to: Jean Leclef
I am very interested in your message.
Do you mean that this board can convert our laserjet 4+ to print grayscale instead of halftone (dithering)?
Does the board go in the printer or in the host computer?
Is a host a Mac or a PC or a Sun or a HP?
Mike O'Keefe
--------------------------------------

for those who are intested in laser printing

the following company in New York has a very good alternative to print
images on Laserjet 3/4/4+
printers - it is a German interface board with 150, 300 and even 600 dpi
printing capability, with a palette of 256 from 1024 gray levels. it works
really fine for printing hires SEM images and prints a 2 MByte image in seconds!

the experience shows that it is a real alternative to photographic images
when you have to find another way to print good pictures from a SEM/STEM.

contact :
Electro Image, Inc.
9, Round Hill Road
LAKE SUCCESS, NEW YORK 11020
tel 516 773 4305
fax 516 773 2955


Jean Louis Leclef









From: Alexander S. Sobolev :      asobol-at-bioprgr.aeiveos.ac.ru
Date: Fri, 17 Nov 1995 08:22:23 +0300 (MSK)
Subject: AFM - DNA-polypeptide complexes

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To: Microscopy-at-Sparc5.Microscopy.Com
Content-Length: 107
Content-Type: text/plain
Message-ID: {ADFm1hmuCL-at-bioprgr.aeiveos.ac.ru}


Thank you,
Alexander Sobolev.





From: JEOL Brussel :      jeolbxl-at-ub4b.eunet.be
Date: Fri, 17 Nov 1995 13:40:47 +0100
Subject: RE : sample charging

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To whom it may concern.

I understand that charging up of samples is always a very problematic item
in SEM. However under certain conditions of your sample and also depending
on required resolution and magnification, LV-SEM (low vacuum SEM) can give a
solution. Even under low vacuum condition excellent X-ray microanalysis can
be done even on light elements.

If you want more detailed please contact us, or your nearest JEOL office.


Best regards.


Bob Hertsens
JEOL Europe BV
Ikaroslaan 7A
B-1930 Zaventem
Belgium

tel : ++(32)2-720.05.60
fax : ++(32)2-720.61.34










From: Beth Trend :      trend-at-cems.umn.edu
Date: Fri, 17 Nov 1995 12:31:47 -0600
Subject: Technician positions available

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Two laboratory technician positions are available in a University analytical
laboratory containing electron microscopes, optical scopes, a Rutherford
backscattering spectrometer, X-ray diffractometers, and scanned-probe
microscopes.

One position is at the Junior Lab Technician level and the other is a Senior Lab
Technician. The duties will include assisting researchers with sample
preparation, maintaining equipment, and assisting users from the University and
from industry. The positions also include routine start-up and shutdown of
instruments, maintaining and distributing supplies, and miscellaneous daily
tasks.

Please contact me directly for further information.

_______________________________________________________________________
Beth Trend trend-at-cems.umn.edu
Coordinator, Characterization Facility University of Minnesota
Center for Interfacial Engineering
100 Union St SE Room 187 phone 612-624-1365 fax 612-626-7530
Minneapolis, MN 55455





From: OptoMech-at-aol.com
Date: Fri, 17 Nov 1995 15:26:48 -0500
Subject: Re: LM/ Hg HBO 50W lifetimes

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Manufacturer lifetimes:

HBO 50W 100hrs.
HBO100W 200 hrs.
XBO 75W 300 hrs.

Lots of people run them much longer than the specified time. There are
potentially two big problems here:

1. After the life of the bulb is reached, the bulb starts a progressive drop
in intensity. The first wavelength affected is the UV and it creeps up from
there. Most people using FITC/Rhodamine, don't notice as much until the bulb
is affected in their wavelength (400+ hours). If you are doing quantitative
work, you will definitely want to change bulbs at the specified times.

2. Stability. The longer the bulb is run, the more the electrode and anode
are worn. As they wear, the arc becomes unstable and dances on the ends.
This causes flickering and some noise that can interfere with patch
clamping. If a bulb explodes in your lamphouse, you will find that you now
have to replace the collector lens, relay optics and sometimes the heat
shields and mirrors. The biggest cause of exploding bulbs is on/off witching
too frequently. A mercury bulb must be allowed to completely cool before be
turned back on. A xenon bulb can be refired while still hot. To prolong the
life of a bulb, it technically should be left on for long periods of time.


It should be noted that Osram has just come out with a new HBO 103 bulb which
is the new replacement for the HBO 100W and is now rated to 300 hours for
about $15-20 more than the old bulb.

Travis Goulette
Opto-Mechanical Consultants
2102 Riding Crop Way
Baltimore, MD 21244
410-944-1721




From: Daniel Possin :      oemlab-at-u.washington.edu
Date: Fri, 17 Nov 1995 12:47:20 -0800 (PST)
Subject: Re: SEM: nebulizer for particles dispersion

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X-Sender: oemlab-at-saul2.u.washington.edu

Ted Pella, Inc. (PELCO #14601, Tel.#800-237-3526) has always supplied a
hand-made glass one which I have used in the past for negative stained
preps with excellent results. It isn't cheap but I think it's the best
I've seen. BTW, I am in no way associated with this company and
have not been solicited by Ted Pella, Inc.in any way.

Dan

On Thu, 16 Nov 1995, Michel DESCHUYTENEER wrote:

} Date: Thu, 16 Nov 1995 14:02:48 +0000
} From: Michel DESCHUYTENEER {deschuyt-at-ccmailg1.sbbio.be}
} To: Microscopy-at-Sparc5.Microscopy.Com
} Subject: SEM: nebulizer for particles dispersion
}
} Fellow microscopists:
}
} I plan to buy a nebulizer to prepare dispersions of particles in the
} 1-10 micrometers range.
} I have seen various models in as many catalogs.
} Can anyone recommended a particular model? Is there one to avoid at
} all cost?
} Thanks in advance for your comments.
} MICHEL
}
} Michel Deschuyteneer deschuyt-at-sbbio.be
} Scientist - Electron Microscopy Laboratory
} SmithKline Beecham Biologicals
} Rue de l'Institut, 89 B-1330 Rixensart Belgium
} Tel: +32-2-656 9290 Fax: +32-2-656 8113
}

%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
% %
% Daniel Possin Work: 206/ 543-7489 %
% Dept. of Ophthalmology RJ-10 FAX: 206/ 543-4414 %
% University of Washington Home: 206/ 778-1714 %
% Seattle WA 98195 USA Email: oemlab-at-u.washington.edu %
% %
% "The chinese expression 'cheung meng ba sui, gong hey fat choy' is %
% equilvalent to Vulcan expression 'live long and prosper'. It's a %
% small universe and getting smaller everyday". %
% %
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%





From: pat_masarachia-at-Merck.Com (Pat Masarachia)
Date: Thu, 16 Nov 1995 16:28:03 EST
Subject: Sodium Vapor Lamp and Autoradiography

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Message-Id: {199511172207.QAA03193-at-Sparc5.Microscopy.Com}

Can anyone tell me how safe sodium vapor lamps are for
autoradiography emulsions? Are special filters needed?
Thanks,

Pat Masarachia
Bone Biology
Merck Research Laboratories
West Point, PA 19486
phone 215-652-7999
e-mail pat_masarachia-at-merck.com






From: sspring-at-usa1.com :      sspring-at-usa1.com
Date: Fri, 17 Nov 1995 18:03:37 +0000
Subject: unsubscribe

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Sender: {sspring-at-usa1.com}

unsubscribe microscopy-at-Sparc5.Microscopy.com






From: Murphy, Judy :      murphy-at-ms.sjdccd.cc.ca.us
Date: 17 Nov 1995 14:35:41 -0800
Subject: Proc in EM, info on manual

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Message-ID: {n1395503519.43018-at-ms.sjdccd.cc.ca.us}

Here is the info on the binder manual that is updated quarterly. I have had
several requests for the info after I sent out the info.

Procedures in Electron Microscopy
A.W. Robards
A.J. Wilson

John Wiley & Sons Ltd
605 Third Ave
New York, NY 10158-0012

ISBN 0 471 928534

Sorry, for not sending this originally, but had to run to library to get it
and didn't have time at that moment.

Judy M.

Judy Murphy, PhD
Dept. of Microscopy
San Joaquin Delta College
5151 Pacific Ave
Stockton, CA 95207

Phone: 209/474-5284
FAX: 209/474-5649
e-mail: murphy.ms.sjdccd.cc.ca.us






From: David S Leaf :      dslmap-at-honeydew.cc.wwu.edu
Date: Fri, 17 Nov 1995 17:28:29 -0800 (PST)
Subject: Proc in EM, info on manual

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subscribe




From: mallamaci-at-goodyear.com (Michael Mallamaci)
Date: Fri, 17 Nov 1995 16:36:24 -0500
Subject: Kevex PDP-11/73 ethernet problem

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X-Sender: t901378-at-rds163
Message-Id: {v01530500acd29f332515-at-DialupEudora}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Dear Fellow Microscopists,

I have encountered an annoying roadblock to getting our Kevex PDP-11/73
minicomputer on the network. For those familiar with this task: I have been

following John Mansfield's documented roadmap for getting these older EDS
systems on ethernet. I've had no problem installing DELQA ethernet card in the
PDP-11/73, but getting the software on our system has been an unexpected
hassle.

The only input into our Kevex box is by way of an Iomega Bernoulli dual
drive, which handles 44MB cartridges. Process Software, the company
which makes TCPware for these computers, can't supply their software on
this media, but instead offers 5-1/4 inch RX50 format or 8 inch RX01
format. Kevex Corporation has graciously agreed to copy the software onto
our 44MB cartridges, but they can only accept 8 inch RX02 double density
format, which Process Software is unable to provide. So, I am sitting here
with the software we need, but apparently with no way of getting it onto
the Kevex...

I have been reading up in my 'DEC microcomputer interfaces handbook' (very
dry and nearly unintelligible text) and it seems that RX01 and RX02 drives
differ only in the available bit densities. The RX01 drive has a bit
density of 3200 bits/inch at the inner track, whereas RX02 runs at either
6400 bpi (double density) or 3200 bpi (single density, essentially
equivalent to RX01). The handbook makes it very clear (ha ha) that the RX02
drive can be configured to write in single or double density mode, so I am
assuming that Kevex's drive writes double density. However, it also says
that when the drive reads, it determines the density of the media, which I
interpret as the drive is able to read either density without a
configuration change. In a private communication from Nestor, he also
confirms that DEC RX02 drives and the DEC driver software can read RX01
floppies, and is curious whether or not Kevex understands their system.

In light of this information, I have contacted Kevex again and discussed
this. They are willing to give the RX01 floppy a try, but they 'aren't sure
if it will work' because they've 'never done this before.' Is there any
real reason to believe that it wouldn't work? Isn't Process Software
supplying their product on RX01 media so as to not handicap those who don't
have an RX02 drive, since RX02 drives can handle both densities? Is anyone
out there familiar enough with these types of data transfer such that they
can counsel Kevex or instruct them on the correct RT-11 commands to perform
the task? I find it hard to believe that it is more complicated than 'copy
dy1:*.* dl1:*.*'....

Does anyone know of another way (besides Kermit) to get this software
loaded onto the 44MB cartridges, perhaps through a 3rd party or something?
The people at Process Software have been very helpful and are actively
pursuing a solution, and they suggested trying the general microscopy
community as well.

Thanks to all in advance for your advice,

Mike Mallamaci

_________________________________________________________
Michael P. Mallamaci mallamaci-at-goodyear.com
Goodyear Tire & Rubber Company Tel: (216) 796-7436
D/415A Analytical Sciences Fax: (216) 796-3304
142 Goodyear Blvd
Akron, OH 44305 USA






From: SALLY STOWE :      STOWE-at-rsbs-central.anu.edu.au
Date: Sat, 18 Nov 1995 10:13:25 EST10
Subject: Re:EM freeze-substitution

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We also substitute in fixative/methanol or acetone on dry ice, but
where possible leave the sample on dry ice for 2 weeks or so, with
the intention that the substitution should happen at dry ice temp,
not during warm-up.
----------------------------------------------------------------------
Sally Stowe |Email: stowe-at-rsbs-central.anu.edu.au
Facility Coordinator |Post:
ANU Electron Microscopy Unit |ANUEMU (RSBS)
Ph 61 6 249 2743 |Australian National Univ.
FAX 61 6 249 4891 |Canberra,
or 249 3808 |AUSTRALIA 0200






From: Chris Jefferies :      chris-at-stowey.demon.co.uk
Date: Fri, 17 Nov 1995 22:31:09 GMT
Subject: Re: RE: Microscopy Manual Online

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Message-Id: {9511172119.AA19829-at-MIT.MIT.EDU}
To: microscopy-at-Sparc5.Microscopy.Com

This is really a very good point, anything put together by users as I
propose would be free, easy to update, free of restrictive copyright, and
you'd always see the latest version when you checked it for a method.

} But doesn't the Procedures in Microscopy manual cost } $500? Presumably an
} online manual maintained by people like us would be free.
}
} } There actually is a manual in microscopy that gets updated quarterly. It is
} } put out by John Wiley and is called Procedures in Microscopy. It comes in a
} } ring binder so it can be easily updated. It is similar to the one in

Chris
--
-----------------------------------------------------------------------------
| Chris Jefferies E-Mail (home) - chris-at-stowey.demon.co.uk |
| (work) - chris.jefferies-at-bbsrc.ac.uk |
| Microscopy page (UK) {URL: http://www.lars.bbsrc.ac.uk/micro/} |
| (USA) {URL: http://metro.turnpike.net/jefferie/} |
-----------------------------------------------------------------------------




From: dietmar.reiter-at-uibk.ac.at (Dietmar Reiter)
Date: Sat, 18 Nov 1995 14:39:49 +0100
Subject: Books or online manuals on micro-photography

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Dear fellows,

I=B4m looking for good books (or online-manuals) on microscopical
photography. Topics: theory, gradation, materials, effects of light on
halogenides, applications in LM, TEM, SEM, comparisons to digital
systems...

Any good hints available? Thanks in advance, -Dietmar-

+++ Dietmar Reiter {dietmar.reiter-at-uibk.ac.at} ++++++++++++++++
+++ Dept. of Zoology and Limnology, University of Innsbruck ++++
+++ Technikerstrasse 25, A - 6020 Innsbruck, Austria +++++++++
+++ ph: (+43)-512-507-6170 (-6161), fax: ..-507-2930 (-2957) +++

... If I would have had more time,
I would have written a shorter e-mail ...






From: Murphy, Judy :      murphy-at-ms.sjdccd.cc.ca.us
Date: 18 Nov 1995 13:39:38 -0800
Subject: Mohr Processor, Recommendations

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Message-ID: {n1395420446.37510-at-ms.sjdccd.cc.ca.us}

For those interested in the continuing and hopefully the ending saga of the
Mohr processor problems reported by Judy Murphy, here is the problem, and
results from our rather extensive by now, tests on films.

I am putting the original message on the bottom so if you haven't seen that
but are interested, you might want to read it. (then again, you might not!)

After I had tried all the tests and removed the different sets of rollers and
tried a mixture of hand chemistry and processor (as described in the original
message, below), the rollers by then were dirty from whatever was being
"fried" onto the surface of the Ektapan (4162) and SO 163. Also by that time
I had gone through at least 150 sheets of film which is the limit for the
solutions so had to change solutions as well. I removed the set of washer
rollers, which is rather large) and put them in cleaning solution overnight as
there was stuff baked all over them. The other sets of rollers I soaked in
water. The next morning, with brush in hand, ready to scrub the rollers as
this stuff was truly "baked" on, I turned over the washer rollers. and found
that there were two pieces of 8 x 10 paper interwoven in the inside set of
rollers.
Thus certain parts of the rollers were not working. Some of them HAD to
be working however, as 4489 and 4127 was being processed OK and all of the
film was being transported properly out of the processor. It turns out that
there is no easy way for the user to remove these sheets as there are abundant
gears etc. which would require some expertise to get all the parts back
together again. That is a bit of a worry to me, in that, if we start to
depend on the processor and get rid of our chemicals, if the rollers jam, we
are DOWN. We have 80 students printing and producing negatives, if we are
down and can't process or print, our instruction programs comes to a grinding
halt - all due to a set of rollers. Something for everyone to think about.
Anyway on with the saga.
I started in my testing all over again with the new set of rollers that
were sent. Mohr shipped them overnight delivery, which was helpful. All of
the films worked. It was just a matter of finding the correct times and
re-doing everything I had done 2 or 3 times before.
I continued to time the unit from leading edge in to leading edge out.
Previously the setting of 2.0 (Extended switch ON) was 14.5 min. With the new
rollars the setting of 2.0 + ext time ON (which should have been longer) was
now 8 min. 3.0 + Ext Time ON was 10 min.
It was obvious that the films were spending more time in the washer/dryer
rollers then they should have. They might have even stopped at some point.
That was unclear. I did not have time to test if all the extended times were
now reproducible but the one on 3.0 minutes was. I did test that with the
extended time OFF, the 2.0, 2.5, 3.0, 3.5, 4.0, 5.0, 5.5, 6.0 and 6.5 were as
they stated.

Times that worked in the trial unit
4489 setting 2.0, Ext Time, OFF
4127 setting 3.5, Ext Time, OFF
Ektapan, 4162, setting 6.5, Ext Time, OFF (see note below)
SO163 setting 3.0, Ext Time, ON

The 600 watt is definitely necessaary for both SO163 and Ektapan.
NOTE: At the above setting for Ektapan, i.e. 6.5 (Ext time, OFF), the film
when it comes out of the processor, is just slightly, very slightly tacky and
requires about 20 sec of RT to dry. Because we have a multi-user, multi-film
facility, I am going to put the processor to the test with the Ektapan at 6.5
(with Ext Time OFF), so students don't have to constantly turn the ext time
on and off. OTHERWISE, it is likely the extended time will be left on when it
should be off and vice versa.
When we process SO163, of course, we will have to use the extended time ON.

It is still unclear to me why the films previously were not being fixed
properly even at the longest times, and now they appear to be. Perhaps there
still IS unexposed AgBr in the films and it is not that obvious because it is
not being baked which certainly made it visible. Definitely the SO163 has a
small amount of stuff left as there is a slight haze on the film. It would be
a good idea to get a Hypo Kit which tests to see if there is any unremoved
material left in the films. Someone mentioned to me about this kit but I have
never seen it. If anyone knows a source, please let me know. If there is
material left in the films then of course the archival quality of the films is
in question.

My suggestion for anyone buying a unit based on what I have had to do.
Ask that the company process Ektapan 4162 on the particular unit they are
about to send you. Ask also that the processed Ektapan be sent with the unit
so you can see them. If 4489 is processed and works there is no guarantee
that the washer rollers are actually working as was demonstrated in the unit I
have. I spent copious hours in the darkroom and went through a great amount
of solutions, film and most importantly time when the apparent culprit was the
washer rollers. Again, that doesn't explain the lack of fix problem
previously explained in the original message. That is still not clear to me.
I hope they will now leave the unit long enough that we can actually use
it to see if it will work in our multi-user facility that does both lots of
film and prints. At the moment I only know what the times are and have worked
out the problems with the unit. The logistics of doing both film and prints
with so many students on the processor is yet to be answered.

Hope some of this has been helpful to someone.
Cheers,
Judy Murphy



Original Message
I have recently been sent a Mohr processor to test to see how it works in our
lab for film and paper as we must get rid of our darkroom. This note is about
the negative processing as I haven't even got to the print processing yet.
Specifically I am having a major problem with Ektapan and SO163 (see below for
specifics)

NOTE: Most of our electron microscopes are analogue so we would like to go
digital but cannot totally do that at this time.

The films we need to have processed are (all Kodak films)
4489 EM film (3 1/4 x 4 inch)
SO163 EM film (twice the speed of 4489) 3 1/4 x 4 inch
Ektapan ( a 4. x 5 sheet film) continuous tone film for making copy negs,
copying pictures, light microscopy in B/W
4127 (4 x 5 sheet film) good continuous tone used for SEM (Our student's can't
afford Polaroid)
The last two films are panchromatic films.

This system allows one to choose the time one processes in each chemical i.e.
Mohr developer and standard Kodak Rapid Fix. This unit has a 600 watt heater
on it. It also has the extended time switch.

We are using a temperature of 88 degrees for the dev and fixer (when measuring
it, the dev is actually 88.5 and the fix is 84.5)

We run several plastic cleaning sheets through the processor before processing
the negatives.

To give an idea of the initial clearing time in Kodak rapid Fix by wet
chemistry, 4489 clears in 20 s in fresh fix; SO163 clears in 37 sec in fresh
fix.

Every session we have worked on the Mohr, we have also developed the negatives
by standard Kodak recommended and tried and proven "wet" chemistry using the
same Kodak fix that is in the processor. All of the "wet chemistry" negatives
have come out fine, as expected.

RESULTS from Mohr
4489
Works fine at the 2 minute setting. dry to dry.

4127. Works fine at 3.5 min. setting.

Ektapan and SO163 are problem childs.
I have tried the Ektapan and SO163 at the following min. settings on the Mohr
machine
2, 2.5, 3, 4, 5, 5.5, 6, 6.5
2 + Extended time (14.5 actual min from dry to dry)
3 + Extended time
6.5 + Extended time (17.5 actual min from dry to dry)
As a rule of thumb, each of the settings on the machine is divided by 4 to get
how much time is actually spent in each place (developer, fix, wash, dry)
Thus the 17.5 actual time is divided by 4, which means 4.4 sec in each place.
The 2 min setting means 30 sec in each place.

Because the first unit I got, the heater was not functionikng, I did my first
tests without a heater. All had to be put in a dryer for about 2 min. to
completely dry which they were. With and without a heater, 4489 and 4127
worked in the same times as indicated above.

Without a heater
Ektapan, 2+Extended Time On (14.5 min. total) appeared to clear the photos,
but wet.
SO163, 5+Extended Time On appeared to clear the photos, but wet.

Heater arrives
4489 and SO163
Did the same tests with all the times, and at all of the times from the
shortest 2.0 (Extended Time OFF) to 6.5+Extended Time ON, it not only didn't
clear it but it precipiated black stuff (which I think is silver) all over the
negatives in a potchy distribution. This could not be cleared anymore in fix
applied outside the Mohr machine or washing outside. It was fried onto the
negs. (Again 4489 worked fine)

I did have fresh fixer and developer. When I ran 4489 in between the Ektapan
and SO163 tests, it still turned out at 2 min. (Ext. Time OFF).

I then removed the wash rack and ran negatives through the Mohr dev and Rapid
fix (in the Mohr machine) and let them drop in the water bath. I did two
things
A. One neg I washed for 30 min. outside the Mohr to be sure it was washed,
then ran it through the wash and dryer roller. The same thing happened.
Stuff precipitated all over the neg. and fried on.
B. One neg I removed after it went through the fix rollers and fixed it
outside, followed by a fix neutralizer and washed it for 20 min outside. I
then ran it through the Mohr wash rollers and dry. It also had stuff on it,
fried to the neg as all of the others, but not quite as bad as those
completely processed in the Mohr. I was very surprised by this results as I
expected that if the unexposed Ag was completely washed out, it shouldn't
happen.

Mohr doesn't have any suggestions and Ted Pella Inc. hasn't heard of this
happening and has no suggestion.
To add to all of this, my darkroom will very shorty be converted into a
room to accept another instrument and I have no way of developing negatives
unless I get a processor to do so. To say the least, my level of frustration
is quite high. I have now spent about 16 hrs. testing this machine in a very
methodical way, and still can't get Ektapan or SO163 to work.
If there are any suggestions or similar experiences, I would really
appreciate them. I really am out of things to try or do.
Thanks
Judy Murphy

Judy Murphy, PhD
Dept. of Microscopy
San Joaquin Delta College
5151 Pacific Ave
Stockton, CA 95207

Phone: 209/474-5284
FAX: 209/474-5649
e-mail: murphy.ms.sjdccd.cc.ca.us





From: cct-at-dsmail.hmi.de (Wang_Wei_Hua)
Date: Sun, 19 Nov 1995 12:52:55 +0100 (MET)
Subject: unsubscribe

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unsubscribe cct-at-dsapp1.hmi.de




From: GREG VAUGHN MARTIN :      gmartin-at-welchlink.welch.jhu.edu
Date: Sun, 19 Nov 1995 11:42:43 -0500 (EST)
Subject: Re: Visual detection of CY-5

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Flow Cytometry {cytometry-at-flowcyt.cyto.purdue.edu}

Hey Daryl --
I have had good results visualizing Cy5 with a 100W HBO lamp on a
standard epi-fluor. 'scope using Zeiss' "00" filterset (excitation:
BP 530-585, beamsplitter: FT 600, emission: LP615) which is a filterset
designed for visualizing Texas Red. However, you can't see cy5
fluorescence by eye. If you have a CCD camera you can use it to capture
the Cy5 image as long as the CCD has decent spectral response above
~650nm. Many CCD cameras have an IR cut off filter so that they will
match human vision. For the camera I use (Dage 72) it was a trivial
matter to remove the IR cut off filter.
Good Luck,

Greg Martin
Dept. Cell Biology and Anatomy
Johns Hopkins School of Medicine

On Fri, 17 Nov 1995, Daryl Webb wrote:

} Hi all, does anyone have experience with CY-5 on a "standard" HBO 50 lamp
} based Fl microscope. I've heard that it can be difficult to see (as opposed
} to detect with pmt's), the local sales droids have never sold the appropriate
} filter block so wont commit themselves. Dont particularly wish to fork out
} for the filter block if I wont be able see my results...
}
} Anyone with advise/experience.
}
} ta muchly
} --
}
} Daryl Webb (dwebb-at-waite.adelaide.edu.au)
} Dept. of Plant Science, Waite Institute
} University of Adelaide, Glen Osmond S.A. 5064
} Australia. Voice:61_8 303 7426 Fax:61_8 303 7102
}
}
}
}




From: tphillips-at-biosci.mbp.missouri.edu (Tom Phillips)
Date: Sun, 19 Nov 1995 14:16:00 -0500
Subject: RE: TEM courses

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Message-Id: {v01510102acd531d82ad9-at-[128.206.15.189]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

In regards to a hot debate we are currently having here, I am curious what
other universities do about teaching TEM courses. My specific questions
are:

1. How often do you teach a TEM course?
2. How many grad/undergrads take it?
3. Do you charge for consumables or beam time?
4. Does it make use of a departmental or campus wide facility? If so,
does it interfere with researchers using the facility?

If you e-mail me directly I will be happy to post a summary of the
responses to the list once they are all in.

Thanks for your input.


Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility
3 Tucker Hall
University of Missouri
Columbia, MO 65211
(314)-882-4712 (voice)
(314)-882-0123 (fax)






From: Nancy Desmond :      nld-at-avery.med.virginia.edu
Date: Sun, 19 Nov 1995 16:15:14 -0500
Subject: deconvolution

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I saw your response to Mike D.'s query. Could you post the phone
number/address for Deltavision please? Someone here has the
Scanalytics package, but it's too slow for our needs. Is the
Deltavision as slow as Scanalytics to deconvolve?


A company called AutoQuant Imaging in Albany, NY, is beta testing
a deconvolution package (3-D) called AutoDeblur-TLB which is
based on Tim Holmes' mathematics. (phone 518.276.2138;
aqisales-at-aqi.com)


--
Nancy L Desmond, Ph.D. nld-at-virginia.edu
Department of Neurosurgery 804.924.5607 (voice) 804.982.3829 (fax)
University of Virginia Health Sciences Center, Box 420
Charlottesville, VA 22908




From: Alex Titkov :      ALEX-at-bunyip.ph.rmit.edu.au
Date: Mon, 20 Nov 1995 10:45:56 EST-10
Subject: Quantitative EDS of C and O in steels

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Dear Microscopists,

How good can be an EDS for quantitative analysis of carbon and/or
oxigen in steels? What detection limit and accuracy should we expect?

Thank you,
Alex
---
Alex Titkov
Electron Microscopy Unit
RMIT Applied Physics
GPO Box 2476V
Melbourne VIC 3001 AUSTRALIA
alex-at-bunyip.ph.rmit.edu.au
Voice:(03) 9660 2205
Fax: (03) 9660 3837




From: Ms McM Marelic [bot] :      Mugette.Marelic-at-sci.monash.edu.au
Date: Mon, 20 Nov 1995 12:04:57 GMT+10
Subject: info required on membrane filters

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Some advice on membrane filter papers would be greatly appreciated
from your group. An honours project is being completed at Monash
University, Clayton Campus using SEM and TEM procedures. Could you
please suggest a membrane filter which has the following
characteristics.
-not dissolved in ethanol (fixing procedure)
-is compatible with spurrs resin (or other eg. epoxy resin)
-if the membrane is not transparent, it must be able to be
dissolved eg acetone (will have seaweed zygotes grown onto
the surface of the membrane, so nothing to toxic
-pore size of around 10 microns(not crucial)
-membrane filter diameter between roughly between 4cm and 10cm


I have approached Millipore who have not been able to suggest
anything diffinite (possibly polycarbonate membranes which are
transparent and so do not need to be dissolved).
If you are aware of a compatible membrane type or a further contact
person who might be able to help, it would be greatly appreciated.

Thank-you
Mugette Marelic
c/o
Monash University Clayton
Department of Ecology and Evolutionary
Biology









From: casix-at-public.sta.net.cn (CASIX)
Date: Mon, 20 Nov 1995 13:02:44 +0800
Subject: *** New Laser & Optics Web ***

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We setup a new Internet WWW site (URL) for technical data and information
about Crystals, Optics and Lasers. Any comments? Anyone know discussion
grups about laser display and/or laser spectroscopy? Thanks.

Crystals: Lasers & Optics:
Nonlinear Optical Crystals BBO-OPOs, KTP-OPOs, ....
BBO, LBO, KTP, KDP, ... Diode Pumped Lasers, ...
Laser Crystal: Nd:YVO4, ... Lens, Prisms, Polarizers
Electro-Optic Crystals Windows, Wedges, Mirrors
Photorefractive Crystals Waveplates, Rotators,
Acousto-Optic Crystals ... Laser Optics, .....

Web: http://www.newsight.com/newsight/casix.htm

CASIX, Inc., PO Box 1103, Fuzhou, Fujian 350014, China
Fax: 86-591-362-1248, E-mail: casix-at-public.sta.net.cn or casixus-at-aol.com





From: SALLY STOWE :      STOWE-at-rsbs-central.anu.edu.au
Date: Mon, 20 Nov 1995 18:00:40 EST10
Subject: EMITECH address

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Can anyone send the address or Austalian agents of EMITECH?

Thanks,
Sally




From: peineau-at-tours.inra.fr (Emmanuelle Peineau)
Date: Mon, 20 Nov 1995 09:54:37 +0100
Subject: unsubscribe

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unsubscribe Microscopy peineau-at-tours.inra.fr

Emmanuelle.peineau-at-tours.inra.fr
tel: 47-42-79-64
fax: 47 42 77 43
INRA PRMD 37380 Nouzilly, FRANCE.





From: Ray Hicks :      rh208-at-cus.cam.ac.uk
Date: Mon, 20 Nov 1995 11:13:32 +0000 (GMT)
Subject: Re: Image analysis

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Hi Christian,
there is an excellent and versatile freeware Image analysis program
(NIH-Image) available from zippy.nimh.nih.gov/pub/nih-image.
It runs natively on macs and powermacs, or apparently under emulation
using Executor on an IBM. There is a list similar to this one that
covers uses of the program and general image analysis run by the
university of minnesota, details of subscription are included in the
about nih-image documentation available on zippy.


Ray


On Fri, 17 Nov 1995
CFILION-at-BRMVM1.VNET.IBM.COM wrote:

} Good morning,
}
} We want to performe precise measurements on samples
} (i.e. hole diameter, distance from center to center etc..)
} using our microscope.
}
} Is there a software that can performe this in an easy way. (i don't need to
} relearn "C").
}
} Any vendor can phone me now ! (on thos subject)
}
}
} Christian Filion
} Mat. eng.
} 514-534-6602
} fax: 514-534-7310
}
} Cfilion-at-BRMVM1.vnet.ibm.com
}




From: shaun.sandow-at-anu.edu.au (Shaun Sandow)
Date: Mon, 20 Nov 1995 20:46:53 +1000
Subject: answers to slot grid problems

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Message-Id: {199511201101.WAA20925-at-anugpo.anu.edu.au}
X-Sender: shaun-at-eccles.anu.edu.au
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Original message was as follows:

I'm looking at serial sections of nerves on formvar (1% in chloroform)
coated slot copper grids (pretty standard stuff). The grids are acetone and
distilled water cleaned several times and coated as per standard (which has
worked consistently well for 4 years). For the past few months I've been
having considerable trouble with what appeared to be focus problems. It now
appears that the film is "shifting". The film is carbon stabilised and
clean; we've tried modifying formvar coating preparations, carbon coating
and specimen storage, but the film movement is still present. It appears
"tight" when on the grid, but appears to shift under the beam (as indicated
by what appeared to be focussing problems). Anyone with a suggestion?

End Result:

We had considered most of the suggestions below already. We are now using 1
mm x 0.5 mm Cu slot grids pre-acid (glacial acetic for 30 seconds) treated,
water rinsed (3X), acetone rinsed (habit), water rinsed (3X), blotted dry
and touched on the dull side with a " coat quick G pen" (Daido Sangyo Co.
Japan - what's in it I don't know, but...) and coated as per standard. This
may be overkill, but it appears to be working well. Thanks to all who sent
suggestions. Hope nobody minds me adding there name to their suggestions
that follow...

Suggestions:

1.
1x2 mm slot grids are notoriously hard to completely stabilize even when
carbon coated. If you are sure of your coating technique, I would begin
viewing each new grid by spreading the electron beam just wider than the
length of the grid slot and irradiate the whole thing for about 10-15
minutes prior to taking a closer look. This will strip the light elements
out of the formvar film and "carborize" it making it more stabile. If
this still is not enough I would consider making thicker films by
increasing the concentration of your formvar solution. 1% Formvar in
chloroform seems a little thin to me even with carbon coating. If you
still have trouble, e-mail me again.

Daniel Possin

2.
When I prepared grids as you described I used a formic acid step. By
cleaning the grids for about 15 seconds in formic acid then rinsing well with
distilled water the surface of the grid was better prepared to accept the
formvar film. We also used the thicker Synaptec type of slot grid. After the
formic acid the grids were cleaned as you described. Remember that the acid
etches the grid so use it for only a short time.

Jack Megill

3.
You might try "Grid Glue"

The recipe can be found under "Tips & Tricks" at
http://www.biotech.ufl.edu/~emcl

Just click the Wizard

Greg Erdos

NB. There' a few suggestions on better binding of films / sections to grids
on this WWW pg.

4.
You might want to switch to another film made from Butvar. It is available
from Electon Microscopy Sciences, Cat. #01185. A 0.25% or 0.3% solution in
chloroform is used, and films are cast from slides onto water the same as is
done with formvar.

Martin B. Garment

5.
We have switched to using Butvar films on slot grids, and find that they
are much more stable. It helps if you pre-treat the grids in a dilute
solution of the butvar (0.1%), not to make a film, but just to get the
metal coated (spread the grids on filter paper in a glass dish, just spray
the grids with drops from a pipette, and let dry--use a fume hood!). Then
do your regular filming procedure. Butvar is available from Electron
Microscopy Sciences 321 Morris Road, Box 251, Fort Washington PA 19034,
800-523-5874, and they also have technical reprints available.

Evelyn Clausnitzer

6.

I would have several concerns. I presume that you are also using mesh grids
periodically and finding no focus problems or specimen drift? Otherwise,
you should consider the possibility of a microscope problem.

Regarding slot grids. Carbon stabilization is essential. Narrow slots
(0.5mm x 2.0 mm) are more stable than wide slots (1.0 mm x 2.0 mm). If a
batch of slots are acting unstable (too little carbon?; contaminated formvar
solution giving films with holes or streaks?) one can try to reduce uneven
beam damage and drift by beginning the grid exam at low power and far from
crossover, previewing a wide portion of the grid while the formvar cooks out
and hopefully becomes more stable. Then at high mag and closer to
crossover, the beam is less likely to overheat the formvar locally and
drifting will be reduced.

We cast formvar films from a 0.5% solution in ethylene dichloride.
Depending on the weather, the same bottle may be good for several months
before acting poorly: holes and streaks, if seen, are a warning that it is
time to open a new stock bottle.We preview new batches of grids under the EM
beam (2-3 grids per film) after
carbon shadowing, but before section collection, to avoid those which are
holey or streaked, as these are likely to be unstable.

Sorry that I don't have a means to exactly quantitate the carbon shadowing
process.
We recently published a chapter on serial section methods in Methods in Cell
Biology, Vol. 48, Academic Press. H. Epstein and D. Shakes, Eds.

David H. Hall

7.
We have found it really valuable to "review" internally some of the
problems others seem to encounter. It makes us better here when it
comes to doing our own in-house work and of course, it makes us better
in giving expert customer technical service when one of our customers
has difficulties.

Here is the synopsis of what my internal people have concluded:

1] Your specimen holder is not tight enough. Is that possible? I am
sure you would have checked this, but this was #1 on the list of the
people in our own in-house laboratory.

2] Sections are too thick, causing a heat buildup.

3] Has *anything* changed, for example, did you change bottles of
Formvar? If yes, then that could be your problem. "Formvar" is a
trade name (owned by Monsanto in the USA) and is not the "generic" term
often times used. The "Formvar" family of resins consists of a broad
number of different products, all the same polymer but with different
molecular weights, etc. Only one or possibly two of these products
have the right characteristics for this particular application. We
know exact what is the correct product when we purchase it to fill our
bottles. I know also that there are some EM suppliers who just don't
understand this need to put into their product bottles the exactcorrect
"Formvar" product.

4] The TEM grids you are using could be a problem also, since we know
that from some source, the slotted grids are not as flat as they ought
to be, causing the section to be position independent in terms of what
is focus. To my way of viewing things, this variation should not be
enough to cause a focussing problem, but others seem to believe other
wise. Have you checked the grids in terms of their flatness?


You can take a look at the range of SPI products, including grids by
looking us up on our site on the WWW, the URL being given below. Our
products are now being distributed by Oxford Instruments in Australia
and you can find the details for contacting them on our "Australia"
agent page via our web site.

Let me know if I can be of any further assistance to you with regard to this
particular problem (or some other one you might pose to us).

Chuck (Charles A Garber)

8.
Your procedure has worked for 4 years (and you carbon coat the Formvar), so
I would question the microscope. Dirty apertures maybe? We use Formvar
coated slot grids (no carbon coating) all the time and deal with occasional
drift by "ironing" the sections and Formvar with the beam. The person that
comes up with something better than Formvar or Butvar will make many
microscopists very happy!
Anyway, I would check the scope.

Beth Richardson

9.
I have two things you might think about. First, are the grids from the
same batch that you were using in past years or are they new? There may be
an adherence problem simply because of a different surface structure.
Second, are you sure that the film is moving? What you describe could be
an instability in the TEM's high voltage.

Russell E. Cook

10.
Just a thought - are you sure it is the film and not another problem? We had
something similar a long time ago which turned out to be the fixing of the
grid in the specimen holder. There was some grease combined with a need
to service the holder (Philips EM300) so the the spring was more 'grippy'
onto the grid. If other workers, or non-filmed grids, do not have the problem
then I guess it is the film (or HT problem?). Good luck

Keith Ryan

11.
Is the movement only seen on slot grids or do you see on small mesh
(200) also? The carbon stabilization should have done the trick and I am just
wondering if the holder itself is unstable in some way.
If focusing is the issue and not lateral movement, one VERY long shot
could be the stability of the objective lens current. Sweeping the
potentiometer knob repeatedly through the focus range can help clear dirt from
the contacts and a shot of electrical contact cleaner behind the panel should
be attempted before moving to more costly diagnostics. After all other
low-cost options are exhausted, call for service.

Doug Davis

12.
I have had good luck with specimens which shift under normal beam
conditions by using very low beam currents (~10 pA). In order for this to
give you a decent image, you need either to have a long exposure time or to
use very sensitive film. LoDose or MRF32 will be sensitive enough, but they
have large grain--there is still no free lunch. If you have a very sensitive
video system, that will make focusing easier--we have an intensified CCD which
gives a useful image at video rates, so focusing can be done in a few seconds.
I suppose that using even a large mesh grid would not be satisfactory, but if
it is, then that may also stabilize your specimen.

Bill Tivol

13.
Maybe this will help. I had a similar struggle a while back, and it turned
out to be the microscope. It was a new Joel, and the beam would charge on
the grid and shift just when taking a picture, causing the photograph to
look as if the tissue was drifting. We convinced Joel it was a microscope
problem, and they fixed it.

John F. Smiley
______________________________________________

Shaun Sandow,
Division of Neuroscience,
John Curtin School of Medical Research,
Australian National University,
ACT 0200

Ph. (06) 249 4782
Fax. (06) 249 2687





From: ENERGY BEAM SCIENCES, INC :      75767.640-at-CompuServe.COM
Date: 20 Nov 95 09:11:57 EST
Subject: Re: SEM: nebulizer for particles dispersion

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In response to Daniel Possin's recommendation of the Ted Pella nebulizer, I have
to agree (and I'm a competitor!). This device was designed by a microscopist,
and is a product custom-built for this purpose. It is expensive because it is
produced in small quantities by a large glassware company who normally make
things in the tens of thousands.
I suggest you contact Ted directly at teppel-at-snowcrest.net, as I believe he has
publications available relevent to this product.
Steven Slap, Energy Beam Sciences





From: mgarment-at-facstaff.wisc.edu (Martin B. Garment)
Date: Mon, 20 Nov 1995 08:30:27 -0600
Subject: TEM donation request

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If anyone can donate a used TEM (less than 10 years old) to be sent to
Vietnam, please contact Dr. Judith Ladinsky, Director of International
Health Affairs at UW-Madison. She can pay for shipping, and can be reached at:
jlladins-at-facstaff.wisc.edu
Martin B. Garment
Ophthalmology and Visual Sciences
1300 University Ave. Rm. 6687
Madison WI 53706
Voice (608) 262-9596
Fax (608) 262-0479
Email - mgarment-at-facstaff.wisc.edu





From: tsr-at-codon.nih.gov (Tom Reese)
Date: Mon, 20 Nov 1995 12:04:58 -0500
Subject: Re: Kevex PDP-11/73 ethernet problem - RX01/RX02 drives

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Message-Id: {9511201549.AA23157-at-MIT.MIT.EDU}
To: Microscopy-at-Sparc5.Microscopy.Com








From: spectral-at-inforamp.net (Spectral Diagnostics Inc.)
Date: Mon, 20 Nov 1995 14:16:07 -0500 (EST)
Subject: SEM immunolabeling

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I would like to have suggestions for labeling intracellular antigen with
gold conjugated antibody. References, techniques etc.





From: ruzin-at-nature.berkeley.edu (Steve Ruzin)
Date: Mon, 20 Nov 1995 13:43:13 -0800 (PST)
Subject: Euparal

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I am looking for a source for an old coverslip mounting medium: Euparal.
Does anyone know where I can find the manufacturer or vendor?

How about Piccolyte?


_______________________________
Steven Ruzin
NSF Center of Plant Developmental Biology
University of California
Berkeley CA 94720-3102
510-642-6602






From: wesaia-at-iastate.edu (Warren Straszheim)
Date: Mon, 20 Nov 1995 09:26:07 -0600
Subject: Re: TIFF

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Tony Garrat-Reed wrote:
} Dear Netters,
{snip}
} The TIFF format is quite precisely defined. It originated
} with Aldus Corp., and was supported actively by them for a while.
} Later, as it became a *de facto* standard, Aldus's support became passive.
} Since Adobe bought out Aldus, they have taken over the support. There
} is lots about TIFF in the Adobe support forum on Compuserve, and I
} expect that there are other places, too, but I don't know them. You
} can download the complete TIFF 6.0 specification via anonymous ftp
} from SGI.COM in directory graphics/tiff. The filename is tiff6.ps
} (it is a postscript file, so should be downloaded as ASCII, and it
} is big!).
}
I will have to check out the reference you just cited. But I will take some
exception. TIFF has much flexibility/variability built into it. As a result,
you might say that there is no such thing as a "standard" TIFF file.
Programmers can choose to include certain fields and omit others. There is
some redundancy in the spec so that the same information can be specified in
different ways.

A robust TIFF reader should be able to handle any combination. However, we
all deal with finite software written at some point in time. It may not be
able to handle some combinations of fields. Some months back, I posted that
I found PageMaker, Microsoft Word, Graphics Work Shop and some other apps
dealt with the images differently. What worked on one app sometimes failed
on another. Hopefully the current versions are more comprehensive.

To the best of my knowledge, the Mac-specific stuff gets added and deleted
as necessary when shipped properly through an application like Fetch. I
suspect difficulties were in the reader-apps on whatever end.
----------------------------------------------------
Warren E. Straszheim
270 MD Ames Lab/ISU, Ames IA, 50011
Phone: 515-294-8187 FAX: 515-294-3091
E-Mail: wes-at-ameslab.gov (or: wesaia-at-iastate.edu)

coal characterization and processing
electron microscopy, x-ray analysis, image analysis
computer applications





From: drazbaj-at-ATHENE.HH.RI.CCF.ORG (Judy Drazba)
Date: Mon, 20 Nov 1995 14:19:35 -0500
Subject: TIRFM

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In response to the query on Total Internal Relection Fluorescence
Microscopy (also known as Evanescent Wave Microscopy):

I found the simplest explanation in a paper by Daniel Axelrod:
J Cell Biol. 89:141-145 (1981).

Two other good papers that include Theory and lots of Math:

TIRFM I, J. Cell Science, 96:219-230 (1990)
TIRFM II, J. Cell Science, 103:491-499 (1992)

Judy Drazba, Ph.D. (drazbaj-at-athene.hh.ri.ccf.org)
Confocal Microscopy Facility, NC-3
The Cleveland Clinic Foundation
9500 Euclid Avenue
Cleveland, OH 44195-5001
Office (216)445-3760
Lab (216)444-8712
FAX (216)444-7927






From: tphillips-at-biosci.mbp.missouri.edu (Tom Phillips)
Date: Sun, 19 Nov 1995 14:16:00 -0500
Subject: RE: TEM courses

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Message-ID: {n1395262450.42768-at-ms.sjdccd.cc.ca.us}
"Tom Phillips" {tphillips-at-biosci.mbp.missouri.edu}
X-Mailer: Mail*Link SMTP-MS 3.0.2

I would appreciate it if all would send their answers to the list server as I
would like to see the original data as well as a summary (my cake and eat it
too).
thanks
judy murphy
_______________________________________________________________________________

In regards to a hot debate we are currently having here, I am curious what
other universities do about teaching TEM courses. My specific questions
are:

1. How often do you teach a TEM course?
2. How many grad/undergrads take it?
3. Do you charge for consumables or beam time?
4. Does it make use of a departmental or campus wide facility? If so,
does it interfere with researchers using the facility?

If you e-mail me directly I will be happy to post a summary of the
responses to the list once they are all in.

Thanks for your input.


Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility
3 Tucker Hall
University of Missouri
Columbia, MO 65211
(314)-882-4712 (voice)
(314)-882-0123 (fax)



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From: William Tivol :      tivol-at-wadsworth.org
Date: Mon, 20 Nov 1995 15:57:24 -0500 (EST)
Subject: Re: Sodium Vapor Lamp and Autoradiography

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} Can anyone tell me how safe sodium vapor lamps are for
} autoradiography emulsions? Are special filters needed?
} Thanks,
}
Dear Pat,
If you are using an x-ray film emulsion, such as that on LoDose
film, you must develop the film in total darkness. When I did autoradio-
graphy, I used NS2T film and put it through an x-ray film processor, so
total darkness was not too burdensome a condition.
Yours,
Bill Tivol




From: Daniel Possin :      oemlab-at-u.washington.edu
Date: Mon, 20 Nov 1995 10:01:34 -0800 (PST)
Subject: Re: Sodium Vapor Lamp and Autoradiography

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X-Sender: oemlab-at-saul4.u.washington.edu

We use a Thomas Safelight w/ the filters they recommend for processing
x-ray films. These filters are much less tranmissive than the normal
Thomas black & white filters but seem to offer complete protection
against any fogging. I have tested Kodak NTB-2 coated slides exposed for
} 24 hours to light from this lamp compared with similar slides kept in a
dark box for the same period and found no apparent difference in
background grain numbers. You will have to contact Thomas for information on
the x-ray type filter set (as I cannot locate currently locate mine). I
hope that this helps.

Dan

On Thu, 16 Nov 1995, Pat Masarachia wrote:

} Date: Thu, 16 Nov 1995 16:28:03 EST
} From: Pat Masarachia {pat_masarachia-at-Merck.Com}
} To: Microscopy-at-Sparc5.Microscopy.Com
} Subject: Sodium Vapor Lamp and Autoradiography
}
} Can anyone tell me how safe sodium vapor lamps are for
} autoradiography emulsions? Are special filters needed?
} Thanks,
}
} Pat Masarachia
} Bone Biology
} Merck Research Laboratories
} West Point, PA 19486
} phone 215-652-7999
} e-mail pat_masarachia-at-merck.com
}
}
}

%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
% %
% Daniel Possin Work: 206/ 543-7489 %
% Dept. of Ophthalmology RJ-10 FAX: 206/ 543-4414 %
% University of Washington Home: 206/ 778-1714 %
% Seattle WA 98195 USA Email: oemlab-at-u.washington.edu %
% %
% "The chinese expression 'cheung meng ba sui, gong hey fat choy' is %
% equilvalent to Vulcan expression 'live long and prosper'. It's a %
% small universe and getting smaller everyday". %
% %
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%





From: dac-at-bio.umass.edu
Date: Mon, 20 Nov 1995 16:27:45 -0500 (EST)
Subject: Sectioning tough plant material

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Microscopists:

Perhaps there is someone out there with some experience in obtaining
sections of very tough plant materials that may have silica or other
crystalline inclusions. I have been requested to help with a project of
sectioning Phragmites stems and rhizomes and the information I received
was that it has so many knife dulling inclusions that this has not yet
been sucessfully accomplished. Of particular interest to us are the
"pigment bodies" that are located in the cells of specimens that grow in
saltwater or brackish areas.

I have recently seen a posting from Dr. Garber at SPI Supplies detailing
the qualities of "materials sciences diamond knives" but wonder if anyone
has other suggestions.

In advance I thank you for consideration,

Sincerely,

Dale A. Callaham
Central Microscopy Facility
University of Massachusetts
Amherst, MA 01003 USA

email: dac-at-bio.umass.edu





From: sbarlow-at-sunstroke.sdsu.edu (Steve Barlow)
Date: Mon, 20 Nov 1995 10:24:45 -0800 (PST)
Subject: Open House, San Diego State U

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contact: Marylou Montross, Astronomy Dept., SDSU telephone:
(619)594-6182
Steve Barlow, Electron Microscope Facility, SDSU
(619)594-4523

Open House, College of Sciences, San
Diego State University

Inner
Space, Outer Space

Visitors to San Diego State University will explore worlds
invisible to the naked eye on Saturday, December 2, 1995, from 6-10 pm when
the College of Sciences at SDSU hosts the second annual "Inner Space, Outer
Space" Open House. Amateur scientists and the general public are welcome
at no charge. Light refreshments and T-shirts will be on sale to raise
money for undergraduate student scientist activities.
Scientists and students of the EM Facility, the Geology Department
and the Astronomy Department will display powerful microscopes, telescopes,
and computers that offer views of the universe ranging from microscopic
parts of a mammalian cell to satellite images of earth and worlds beyond
our own planet.
Visitors to the EM Facility in the lower level of the Physical
Science building will see Inner Space-- intricate details of the surface
and internal structures of plant and animal life magnified 1,000 to 50,000
times on the transmission and scanning electron microscopes. Students will
explain the operation of the microscopes and demonstrate the same sample
preparation techniques used to prepare tissue samples from biopsies at the
area hospitals. Remote access from area classrooms to the electron
microscope via Cox Cable TV or the Internet will also be demonstrated.
On the rooftop of the adjacent Astronomy Department, several
refracting and reflecting telescopes will be focused on Saturn, the moon,
and other celestial bodies of Outer Space. The public is invited to an
exciting planetarium show featuring San Diego's night sky. An astronomer
will identify the heavenly bodies visible from your own back yard over the
course of a year. Also on view will be a laserdisc show of images from the
Voyager space probe as it passed by Saturn and Jupiter. Members of the
Astronomy department will provide lively commentary.
The Chemistry/Geology Computer Lab will exhibit satellite images of
the earth as seen from Outer Space, as well as images from the Jupiter
Galileo probe, which is scheduled to enter the atmosphere of Jupiter on
December 7. Members of the Geology Department will discuss how satellite
imaging helps us to understand our own world, as well as worlds far away.
To reach the Inner Space, Outer Space displays, take route 8 to the
College exit and turn south to San Diego State University. Free parking is
available on the top level of the College Avenue Parking Structure. Take
the pedestrian overpass across College Avenue and proceed right, to the
Chemistry/Geology building. Follow the signs to the laboratories located in
the adjacent Physics and Physical Science buildings. The Open House will
take place rain or shine, as most of the exhibits are indoors.

For more information, call Marylou Montross at 594-6182.


---------------------------------------------------------------------------
------------------------------------------------------------------------------
Dr. Steve Barlow
EM Facility/Biology Dept.
San Diego State University
San Diego CA 92182-4614
phone: (619) 594-4523
fax: (619) 594-5676
email: sbarlow-at-sunstroke.sdsu.edu





From: Gary Login :      glogin-at-bih.harvard.edu
Date: Fri, 17 Nov 1995 09:31:33 -0500
Subject: Microwaves (hardware ?)

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Microscopists,
Below is my original post regarding questions about microwave hardware. A
summary of responses follows. Thanks to all who responded.

I checked with our safety dept. and they don't have a policy about
microwaves (except that they be UL listed and unmodified). If a hazardous
chemical is going to be "cooked", then safety expects that the microwave
will be placed in a fume hood. The only problems that safety has had with
microwaves is when people start the microwave and don't monitor it's
progress (to view a WWW page that demonstrates what happens to a simple
table grape when it's microwaved too long, go to:
http://www.sci.tamucc.edu/~pmichaud/grape/).

Doug Cromey

-----original posting-----

Microscopists,
I am responsable for a small histology service lab. My new histologist has
some experience with microwave stain techniques and use of microwaves to
express antigens in archival-paraffin-embedded material. We are seriously
considering the purchase of a microwave for our lab to speed things up, but
we are wondering about the merits of "off the shelf" microwaves (eg
appliance stores) versus "technical" microwaves available from a number of
reputable microscopy suppliers. There is a considerable price difference
between the two types and I'd like to be educated on the relative merits of
one versus the other (our needs are not likely to include EM processing,
since there is a seperate lab in the Health Sciences Center for that).

Perhaps it would be best if you replied to me personally and I can then post
a summary to the list at a later date.

A note to vendors: I appreciate your input, to save yourself paper &
mailing costs, you might inquire if I already have some of your materials
regarding microwaves before you send me any more.

Thanks,
Doug
.....................................................................
: Douglas W. Cromey, M.S. Dept. of Cell Biology & Anatomy :
: Sr. Research Specialist University of Arizona :
: (office: AHSC 4212A) P.O. Box 245044 :
: (voice: 520-626-2824) Tucson, AZ 85724-5044 USA :
: (FAX: 520-626-2097) (email: dcromey-at-ccit.arizona.edu) :
:...................................................................:

-----response-----

Doug:
We use a plain, kitchen-type mw for our processing (principally fixation for
light microscopy and EM, and specifically, to enhance fixative penetration).
We have had no problems, and have had really nice fixation (as long as the
undergrad student doesn't forget the water ballast!) We have not checked
for hot-spots (with a little rack of neon lamps), but use the center of the
ovenfor our material). I'd call us happy with the "low tech" approach. But
if I had a protocol that called for organic solvents, I wouldn't hestitateto
use the lab-style. One good oven explosion could spoil your whole day...
HTH
Julian Smith III
Biology
Winthrop University

-----response-----

In response to Doug's letter, let me add that the Fire Safety Officer here
at the Health Sciences Centre has disallowed our microwave oven for use in
the fume hood because it's only a domestic grade, and he insists that it is
a fire hazard because volatiles might get into the electronics, even though
it is in a very high volume fume hood. I think perhaps that we are the
only lab in the world that has this problem, and I'd like to hear from
others (as many as possible) to prove to the fire safety people that the
hazard is basically nil. Please let me know what the situation is in your
labs.

Garry Burgess
Charge Technologist - Electron Microscopy
Health Sciences Centre
Winnipeg, Canada

-----response-----

Hi Doug,
We are am EM facility and we currently use an older GE. All you need to do
is spend a day calibrating it and finding it,s various hot spots. The
"Scientific" models are designed to reduce these hot spots but at a cost. A
simple way to find them in both types is to place a sheet of styrofoam on
the bottom, run the microvave, and map the places where it begins to melt.
You can get moe of a 3 dimensional picture by elevating the styrofoam. I
have also seen somewhere a calibration pad sold by some of the EM suppliers
although I don't remember where. More modern micros have a more even heating
pattern. I hope this helps.

} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {
Scott D. Whittaker 218 Carr Hall
Research Assistant Gainesville, FL 32610
University Of Florida ph 904-392-1295
ICBR EM Core Lab fax 904-846-0251
sdw-at-biotech.ufl.edu http://www.biotech.ufl.edu/~emcl/

-----response-----

Doug-
As you asked, I am a vendor responding to see if you have our material on
our laboratory microwaves. For a general, and totally non-commercial,
discussion of the merits of laboratory microwaves in general, see our home page:
http://www.mwrn.com/ebs/ebs.htm

I also recommend the Microwave Cookbook for Microscopists.
Steven Slap, Vice-President

-----response-----

Dear Doug,
The key advantages of laboratory microwave ovens over domestic
microwave ovens are:
1) safety features (such as a high volume exhaust)
2) enhanced programming features (such as microwave cycle time
or duty cycle, heating/cooling cycles, more control over time
and power function)
3) accessories (such as computer control and monitoring, water
circulators, vaccuum processing)

There is also a common element between domestic and laboratory microwave
ovens- they are both large cavity devices. The significance (regardless of
advertising claims) is that microwaves are distributed nonuniformly in the
cavity and in the specimen (e.g., slide sections, tissues).

There are several published methods to easily determine how uniform and how
reproducible a particular microwave oven model is. In my experience, it is
important to select a microwave oven based on its uniform distribution of
microwave energy. The current literature in microwave-accelerated specimen
preparation also places more emphasis on calibrating the microwave oven for
the particular application than on the hardware. (Please contact me if you
need this literature- 4 books, several articles)

Gary R. Login, D.M.D., D.M.Sc.
Assistant Professor of Oral Pathology
Beth Israel Hospital
Department of Pathology
330 Brookline Avenue
Boston, Massachusetts, 02215

glogin-at- bih.harvard.edu
Telephone: 617-667-2034
Fax: 617-667-8676

-----end responses-----
.....................................................................
: Douglas W. Cromey, M.S. Dept. of Cell Biology & Anatomy :
: Sr. Research Specialist University of Arizona :
: (office: AHSC 4212A) P.O. Box 245044 :
: (voice: 520-626-2824) Tucson, AZ 85724-5044 USA :
: (FAX: 520-626-2097) (email: dcromey-at-ccit.arizona.edu) :
:...................................................................:





From: skurland-at-gatan.com (Sheri Kurland)
Date: Mon, 20 Nov 1995 18:28:15 -0800
Subject: unsubscribe

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unsubscribe & Happy Thanksgiving






From: cel1-at-lehigh.edu (Charles Lyman)
Date: Mon, 20 Nov 1995 22:32:35 -0500
Subject: TEM Courses

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Tom Phillips and Judy Murphy,

You requested the following information about the Lehigh University
Electron Microscopy Courses:

1. How often do you teach a TEM course?
==} Basic SEM and TEM undergraduate course taught in fall semester each
year. Advanced TEM taught every other year in the spring semester,
alternating with Advanced SEM and X-ray Microanalysis.

2. How many grad/undergrads take it?
==} 15-30 students take the undergrad SEM/TEM course, aand perhaps half of
these are actually graduate students. About 10 graduate students take the
Advanced TEM course and the Advanced SEM and X-ray Microanalysis course.

3. Do you charge for consumables or beam time?
==} Both are charged to the courses making them rather expensive to run
since each course has approximately 10 different laboratories.

4. Does it make use of a departmental or campus-wide facility? If so,
does it interfere with researchers using the facility?
==} All electron microscopes are in the campus-wide central facility
located in the Dept. of Materials Science and Engineering. All departments
including biology and geology use the instruments in this department. In
addition to our six research electron optics instruments, we maintain three
1970s-vintage microscopes for use by our undergraduate and graduate
students: a Philips EM301 TEM and two ETEC Autoscan SEMs.

CE Lyman

Charles E. Lyman Phone: (610) 758-4249
Prof. of Materials Science and Engineering Fax: (610) 758-4244
Lehigh University E-mail: cel1-at-lehigh.edu
5 East Packer Avenue
Bethlehem, PA 18015-3195






From: llsutter-at-mtu.edu
Date: Mon, 20 Nov 1995 21:13:56 -0500
Subject: Mac's & TIFF (was:TIFF)

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I read with interest Tony Garratt-Reed's recent posting on TIFF
files and certainly concur with his assertions. It seems that his note was
in response to a question that I did not receive or accidentally deleted
without reading. However, I would like to elaborate slightly on his
comments relative to Mac's and TIFF files.

The TIFF format is precisely defined. However, my observation has
been that applications to read TIFF files are not. This is especially true
in the case of software produced by instrumentation vendors. Many vendors
produce file formats that comply with the TIFF specification and they
produce "limited" TIFF readers that can read these files. These same
files, when read by a truly generic TIFF reader, will be read and
interpreted. However, the inverse is not always true. It is likely that
you will come across other TIFF files that also comply with the TIFF
specification but can not be read by the same "limited" TIFF reader. I
have encountered this problem when taking images from our SEM image
analysis system to our LM image analysis system. The solution has been to
import the original image into a program such as Adobe's Photoshop and then
rewrite the image to a new file. Although this is not a universal
solution, it usually produces a TIFF file that can be read by a wider range
of TIFF readers. I do agree that the TIFF reader in NIH Image seems to be
more robust than I have found in any other image analysis software.
Relative to the file type and creator information added to a Mac file, I am
not enough of a ResEdit user to know how this information is incorporated
into the file. However, I would be very surprised if this information was
included in the TIFF file header. My impression is that this information
is included "externally". I say this because I have taken TIFF, Word,
WordPerfect, and Excel files to and from Mac's and PC's with no problem.
If the file type and creator information is organically imbedded in these
files, I would not expect the file transfer to proceed as smoothly as it
does.

Personally, I have had no problem bringing TIFF files into or out
of a Mac. In fact, I use the Mac to get a usable TIFF file to bring to a
number of IBM/PC applications. Between NIH Image and Photoshop, I have
read every TIFF file that I have come across and in turn, wrote each to a
PC format disk for export to an IBM/PC or clone. I have also used the Mac
to convert TIFF files to JPEG or GIF images using Photoshop and Word for
Word, respectively. I have exported these images to PC's and UNIX systems
with no problem. An example of an image that was converted from TIFF To
GIF can be seen on my home page (Yes, this is a cheap plug to get you to
look at my home page that is still in a state of development, like
everyone). As a side, I use the Access PC software for Mac to PC
conversion. Although the PC Exchange software that comes with System 7.5
is supposed to do the same thing as Access PC, I haven't had universal
success with it as compared to Access PC.

As for Fetch, I usually transfer files in the Raw Data format. I
haven't had any problems with this approach. However, I only use Fetch
going to and from UNIX and a Mac. I haven't sent or received any files
that were to be used on a PC. I SneakerNet all of my PC images. Another
nice aspect of Access PC is that it supports removable media hard drives
such as Syquest.






Larry Sutter
Michigan Technological University
Dept. of Civil and Environmental Engineering
1400 Townsend Dr.
Houghton, MI 49931

voice: 906-487-2423
FAX: 906-487-2943

e-mail: llsutter-at-mtu.edu
WWW: http://www.civil.mtu.edu/~llsutter/





From: South Bay Technology :      73531.1344-at-CompuServe.COM
Date: 21 Nov 95 00:17:28 EST
Subject: Tripod Polisher User's Group Meeting - MRS

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South Bay Technology will be hosting a VERY informal Tripod Polisher User's
group meeting at the MRS Conference in Boston. The MRS meeting will be held
from Nov 28 - Dec 1 at the Marriott at Copley Place.

The User's Group Meeting will be held in the South Bay Technology Booth on
Thursday November 30. South Bay Technology's booth (booth #1) is located in
the Atrium Lounge in the Marriott at Copley Place.

All interested parties are welcome! We'll look forward to seeing you there!

David Henriks TEL: 800-728-2233 (toll-free in USA)
South Bay Technology, Inc. 714-492-2600
1120 Via Callejon FAX: 714-492-1499
San Clemente, CA 92673 USA e-mail: 73531.1344-at-compuserve.com
sbt-at-msa.microscopy.com





From: South Bay Technology :      73531.1344-at-CompuServe.COM
Date: 21 Nov 95 00:17:39 EST
Subject: NEW Tripod Polisher Workshop

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Workshop on Tripod Polishing

Workshop Objective
This course will cover all aspects of pre-thinning and focus on final thinning
via Tripod Polishing. Due to the limited class size and the extensive hands-on
opportuinities, this course is well suited to novices as well as advanced
Tripodders. The course will include sections on:

How to do it and why should I?
What's really going on and what am I really seeing?
How to prepare small, specific area cross-sections.
The problem of wildly differing materials (eg tungsten).
Rapid preparation of TEM cross-sections.
Preparation of a wide range of materials: semiconductors, ceramics, metals,...

Hands-on Opportunity
This course will be unique in that it will provide a hands-on opportunity for
every class participant. Tripod Polishers, Polishing Wheels, and pre-thinning
equipment will be made available to all participants and actual samples will be
prepared - by the students - as part of the course. This is a great opportunity
to get your hands dirty and actually learn by doing. The instructors will walk
you through each step of the process and then let you loose on the equipment.
This course is designed to teach the Tripod Polishing technique. Silicon
samples will be provided to the students and used as the basis for the course
teaching.

Workshop Location and Dates
South Bay Technology - San Clemente, CA
Dates: Friday & Saturday - February 23-24, 1996

Previous Participants (partial list)
INTEL, AMD, Motorola, LSI Logic, Conner Peripherals, Univ of Maryland, Univ of
New Mexico, UNAM (Mexico), LG Electronics (Korea), Battelle, MEMC, MVA Inc.,
Univ of Michigan, U.S. Bureau of Mines.

Class Size
Due to the intensive hands-on aspects of this course, class size will be
strictly limited to 10 participants.

Registration Fee: $795 (includes lunches and Friday night Dinner)
$695 if registration fee paid by January 12, 1996

Registration Deadline: January 30, 1996

For additional Information: Monica Pflaster
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673
TEL: 800-728-2233
FAX: 714-492-1499
e-mail: sbt-at-msa.microscopy.com




Registration Form

To register for the workshop, please fill out this form and send it, with
registration fee, by January 26, 1995 to:

South Bay Technology, Inc.
Workshop on Tripod Polishing
1120 Via Callejon
San Clemente, CA 92673

Payment must be made in the form of a check, money order, Visa or MasterCard.
Checks must be drawn on a U.S. Bank and made payable to South Bay Technology,
Inc. Credit card orders by FAX may be sent to South Bay Technology at
714-492-1499. Full refund available up to January 12, 50% refund thereafter.
No refunds after January 30, 1996.


Name:


Affiliation:


Address:




City: State:
Zip: Country:_________
Telephone: FAX:

e-mail:________________________

Primary sample type:




VISA MasterCard Card #_________________________________

Expiration Date________ Signature of Cardholder_________________________

Cardholder name (Please print):________________________________________





From: mary-ellen harper (biochem) :      mharper-at-labsun1.med.uottawa.ca
Date: Tue, 21 Nov 1995 07:29:53 -0500 (EST)
Subject: subscribe

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Message-Id: {9511211012.AA02585-at-carbon60.fysik.dtu.dk}

Please register me for a subsciption.
Mary-Ellen Harper, Ph.D.
Assistant Prof., Biochemistry
University of Ottawa
Canada




From: jeanne_barker-at-Merck.Com (Jeanne Barker)
Date: 21 Nov 1995 09:53:18 U
Subject: Unsubscribe

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11/21/95
9:38 AM
Unsubscribe
Please unsubscribe.
Thank-you.
Jeanne






From: Fermin, Cesar :      fermin-at-TMC.Tulane.Edu
Date: 21 Nov 1995 08:10:23 -0500
Subject: Photomicrography Refs.

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Message-Id: {n1395180223.77876-at-msmail.tmc.tulane.edu}


In response to the microphotography manuals here are references from my
EnDNote library, that I use for workshop teaching and lectures. Those from
Olympus are cheap and probably free from your local Rep. or main office
(1-800-446-5967 ask for Ms. Kelly who in the past send me several free
samples).

(Kodak, 1980; Kodak, 1981c; Kodak, 1981a; Kodak, 1981b)
(Abramowitz, 1985; Abramowitz, 1987; Abramowitz, 1990; Abramowitz, 1993)
(Olympus, 1994c; Olympus, 1994b; Olympus, 1994a; Olympus, 1994d)
(Inoue, 1987).
==================================================================
I am not associated with the company that makes the product(s)!
The information provided only constitute a professional cortesy!
==================================================================
***********Cited References:

Abramowitz M. (1985). Microscope Basics and Beyond. ed.) New York: Olympus
Co.,

Abramowitz M. (1987). Contrast methods in microscopy. Transmitted light. ed.)
New York: Olympus Co.,

Abramowitz M. (1990). Reflected light microscopy. An Overview. ed.) New York:
Olympus Co.,

Abramowitz M. (1993). Fluorescence Microscopy. The essentials. ed.) New York:
Olympus Co.,

Inoue S. (1987). Video Microscopy. ed.) New York: Plenum Press,

Kodak. (1980). Photography through the microscope. (8th ed.) NY: Eastman Kodak
Company, 96 pages.

Kodak. (1981a). Kodak Color Films. (8th ed.) NY: Eastman Kodak Company, 95
pages.

Kodak. (1981b). Kodak filters for scientific and technical uses. (3er ed.) NY:
Eastman Kodak Company, 95 pages.

Kodak. (1981c). Using Filters. (8th ed.) NY: Eastman Kodak Company, 95 pages.

Olympus. Applications of fluorescence microscopy. In: New York: Olympus
Optical Co., LTD, 1994a:

Olympus. Basics of the optical microscope. In: New York: Olympus Optical Co.,
LTD, 1994b:

Olympus. How to improve photograpy throught the microscope. In: New York:
Olympus Optical Co., LTD, 1994c:

Olympus. The use of the olympus fluorescence microscope. In: New York: Olympus
Optical Co., LTD, 1994d:


******************************************************************
*Cesar D. Fermin, Ph.D \|T|/ Fax (504) 587-7389 *
*Tulane Medical School /|M|\ Voice Mail (504) 584-2618 *
*Pathology/SL79 \|C|/ Secretary (504) 584-2436 *
*New Orleans La 70112-2699 /|*|\ Lab/Techn. (504) 584-2521 *
* {Fermin-at-tmc.tulane.edu} Dir. Morphological Services *
******************************************************************






From: South Bay Technology :      73531.1344-at-CompuServe.COM
Date: 21 Nov 95 11:47:32 EST
Subject: Correction-Tripod Users Group

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I should probably type my e-mail messages before I have my wine with dinner!

To answer the many questions I have received, the Tripod Polisher User's Group
Meeting will be held during the MRS meeting in Boston as follows:

Boston Marriott at Copley Place
South Bay Technology, Inc. Booth
Booth #1 in the Marriott's Atrium Lounge
TIME: 8:30 am
Thursday November 30, 1995

I hope this clarifies things for everyone! I hope to see you there.

Best regards-

David Henriks TEL: 800-728-2233 (toll-free in USA)
South Bay Technology, Inc. 714-492-2600
1120 Via Callejon FAX: 714-492-1499
San Clemente, CA 92673 USA e-mail: 73531.1344-at-compuserve.com





From: Glenn Poirier :      GLENN_P-at-geosci.lan.mcgill.ca
Date: Tue, 21 Nov 1995 12:01:08 EST5EDT
Subject: Parts for carbon coater

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X-SMTP-Posting-Origin: lansend.cc.mcgill.ca (lansend.CC.McGill.CA [132.206.37.4])
Message-Id: {199511211703.MAA28492-at-sirocco.CC.McGill.CA}

Greetings Microscopists

Would anyone know where I could get a used diffusion pump heater
for an Edwards EO4 diffusion pump. I've ordered the part from
Edwards but its going to be a month before I get it. It's hard to run
a microprobe lab without a carbon coater. If anyone has any
suggestions reply directly to me.

Thanks

Glenn
**********************************************************************
* Glenn Poirier glenn_p-at-geosci.lan.mcgill.ca *
* Electron Microprobe Lab Phone: (514) 398 6774 *
* Earth and Planetary Sciences Fax: (514) 398 4680 *
* McGill University THERE ARE THREE SIDES TO EVERY STORY; *
* Montreal, Quebec YOUR SIDE MY SIDE AND THE TRUTH *
**********************************************************************




From: huffe-at-pgL7.chem.nyu.edu (Edward J. Huff)
Date: Tue, 21 Nov 1995 08:48:06 -0500
Subject: Re: Mac's & TIFF (was:TIFF)

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I read the recent posts on TIFF and have two comments regarding Mac
file formats and the Mac FTP client "Fetch". The bottom line is that
Fetch by default stores Mac files in a "wrapped" format, either
MacBinary or AppleSingle. These files are not TIFF files, but they
contain TIFF files. To avoid this, specify "Raw binary" format when
sending files using Fetch. If you specify "Ascii" format, then pixels
with value 13 get changed to 10 and vice versa.

} A file either is or is not a TIFF file. If it is, then a
} properly-written reader can read either the so-called MAC format
} or the equally vaguely named PC format (the first two bytes of the
} TIFF file define which order the bytes of multi-byte data are stored
} in, and the reader has to read and act on these). I'm not an expert
} MAC user, but I believe the problem with copying files to and from MACs
} is that MACs add extra information to files to indicate the source
} software or the type of file. These files are not, by definition,
} TIFF files! There is nothing wrong with a program doing this, but it
} shouldn't claim that the result is something that it is not! This seems
} to me to be the source of the confusion over TIFF formats. (Perhaps a
} MAC user could comment here?)


The "Mac" vs. "PC" format code in a TIFF file actually specifies how
multibyte integers are stored: either least significant byte first
("little-endian", DEC PDP/11 format), or most significant byte
first ("big-endian", IBM System/360 format). One might guess that
the distinction arose when DEC decided to be different from IBM in
the 1960's. The PC format descends from the DEC format and
the Mac format descends from the IBM mainframe format. (Or maybe
it arose from the right-to-left vs. left-to-right writing order of
Arabs vs. Romans. When adding, we write numbers right to left...).

The TIFF spec permits integers to be stored in either format, but
the same format must be used consistently throughout the file.
Conforming TIFF readers are required to support both integer formats.

The important problem arises when Mac files are stored on PC or
Unix file systems for the purpose of retrieval to and complete
regeneration on the final destination, another Mac. This can
happen either on purpose, or inadvertently, and it has nothing to
do with the byte order of integers.

When stored on a Mac file system, files consist of three parts:
8 bytes of "creator code" and "file type", a data fork (which is
the entire file on PC or Unix file systems), and a resource fork
which would be empty for TIFF files. The creator code and file
type are not essential unless you want to double click the file,
all they do is determine what icon will be displayed for the file
on the desktop, and which application will be launched to open the
file if you double click it. If the 8 bytes are all '?', the file
will be shown using a generic document icon and double clicking it
will give an alert telling you there is no application to open it.

When Fetch retrieves a file from a PC or Unix file system, and you
specify that the file is a Raw binary file, it will give you a
chance to specify the 8 bytes of creator code and file type if you
know what you want. For instance, you can make the creator code
"Imag" and the file type "TIFF" and then the NIH Image TIFF icon
will be shown and double clicking the file will start a copy of
NIH Image if you have one. If you have more than one, it will start
whichever version it feels like.

If you use Fetch and do not specify raw binary mode when sending
it to a Unix or PC file system, then Fetch will pack all of the
parts of the Mac file into either "MacBinary" format or "Apple
Single" format. This permits another Mac to retrieve the file
from the Unix or PC file system and regenerate all three parts
of the Mac file in its file system. This is not what you want
if you want to use the file on the Unix or PC system. One symptom
of this happening is that the file gets larger by 128 bytes.

If you wish to send a Mac TIFF file to a Unix or PC system for use on
that system, be sure to specify "Raw" format when using Fetch.
Otherwise, Fetch will not create a TIFF file on the remote file system.
It will create a MacBinary or AppleSingle file that happens to contain
a TIFF file. Your Unix or PC program will then try to read the MacBinary
file and say (surprise!) this is not a TIFF file.




From: bsgphy3-at-uconnvm.uconn.edu (JIM ROMANOW)
Date: Tue, 21 Nov 1995 17:37:31 -0400
Subject: X-Ray detector parts or whole

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Message-Id: {v01510105acd7f6a007a3-at-[137.99.40.87]}
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Fall Cleaning?

I am looking for a used Kevex x-ray detector (any condition) late 70's and
up with a +35 degree take-off angle for parts. I need the ball shaped
fitting that goes between the LN2 dewar and the detector assembly but will
gladly accept a whole unit.

If you can help please e-mail or call:
(860) 486-2914

Thank you.


Jim Romanow
Electron Microscopy Facility
Physiology and Neurobiogy Department
The University Of Connecticut
Storrs
bsgphy3-at-uconnvm.uconn.edu






From: knoff-at-lipovx.lbl.gov (Laura Knoff)
Date: Tue, 21 Nov 1995 14:40:15 -0800
Subject: NCSM Meeting

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Message-Id: {199511212140.PAA07575-at-Sparc5.Microscopy.Com}
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WHAT: The winter meeting of the Northern California Society for Microscopy.

WHEN: Thursday, Dec. 7 from 5:30 to 9:30 p.m.

WHERE: Casablanca Banquet Facility, 979 San Pablo Ave., Albany, CA

WHO: Dr. John Mardinly Intel, Corp.

"Uses of Microscopy in the Microelectronics Industry"
&
Dr. Richard Demaree and Rick Giberson
Cal State U. at Chico Ted Pella, Inc

"Microwave Tissue Processing for Electron Microscopy"

The Northern California Society for Microscopy (NCSM) welcomes new
members. Anyone interested in microscopy of any type, from atomic force to
optical will benefit from joining the NCSM. We meet four times a year to
have dinner and hear about new developments in microscopy. Dues are very
reasonable at only $15 a year for regular members, and $8 for students. If
you are interested in joining, or knowing more about NCSM, please contact
Kent McDonald by phone at 510-642-2085 during normal business hours, or by
email at: klm-at-uclink4.berkeley.edu If you are interested in attending the
Dec. 7 meeting described above, please call 510-486-4088 no later than Dec.
1 so we can reserve the appropriate number of dinners. Complimentary
drinks will be provided by Ted Pella, Inc. and Philips Electronic
Instruments and the dinner will be $16 for members, $8 for students. Please
join us.






From: JIM ROMANOW[SMTP:bsgphy3-at-uconnvm.uconn.edu]
Date: Wed, 22 Nov 1995 08:58:26 +-900
Subject: X-Ray detector parts or whole

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You can contact Kevex directly at 415-591-3600. They still support =
their full line of detectors so this should be no problem. Since you =
will need to break vacuum to change the mount, it may be best to send =
the detector to Kevex and they can change the mount and ensure correct =
performance before returning it to you.




----------

Fall Cleaning?

I am looking for a used Kevex x-ray detector (any condition) late 70's =
and
up with a +35 degree take-off angle for parts. I need the ball shaped
fitting that goes between the LN2 dewar and the detector assembly but =
will
gladly accept a whole unit.

If you can help please e-mail or call:
(860) 486-2914

Thank you.


Jim Romanow
Electron Microscopy Facility
Physiology and Neurobiogy Department
The University Of Connecticut
Storrs
bsgphy3-at-uconnvm.uconn.edu








From: Carolyn Emerson :      cemerson-at-kean.ucs.mun.ca
Date: Wed, 22 Nov 1995 09:33:38 -0230
Subject: Photomicrography Reference

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Sender: cemerson-at-kean.ucs.mun.ca

Several years ago the Microscopical Society of Canada published a book
by Stanley Klosevych - "Principles and Practice of Microscopy and
Scientific Photography". Information on cost can be obtained from our
Executive Secretary, Marie Colbert, by email to
colbertm-at-fhs.mcmaster.ca
I had noted several recent postings on references to photmicrography
resources. Carolyn Emerson, Editor, MSC Bulletin




From: MARCFRIEDMAN-at-delphi.com
Date: Wed, 22 Nov 1995 09:57:30 -0500 (EST)
Subject: Jamin-Lebedeff Set for Zeiss M'scope

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An associate with a microscope collection is offering for sale
a Jamin-Lebedeff set for a Zeiss Photomicroscope (fixed tube
length) or other Zeiss scope with similar nosepiece and
condenser mounts.

If interested, please contact Mort (Abramowitz) directly at
(516) 844-5049 between the hours of 9:30AM and 3:00PM (Eastern
Daylight Time) on business days.




From: Gib Ahlstrand :      giba-at-puccini.crl.umn.edu
Date: Wed, 22 Nov 1995 10:56:02 -0600
Subject: Minnesota Microscopy meeting.

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MMS NOVEMBER 1995 MEETING:

Imaging Muscle: From 3-D To 2-D & Back Again

Dr. M. Ann Goldstein, Baylor College Of Medicine
MSA Current-President

November 30, 1995

University of Minnesota, St. Paul Campus
Student Center(2nd floor) - Cherrywood Room

5:00 - 6:00 Social Hour with Appetizers
Cheese - Crackers - Wines - Sodas - Mineral Waters

6:00 - 7:00 Customized Dinner Buffet
Herbed Chicken Breast
Stir Fry Vegetable Blend - Mixed Salad Greens
Whipped Potatoes and Gravy - Assorted Breads
Coffee, Tea, Milk - Dessert

7:00 - 8:00 Presentation, Dr. M. Ann Goldstein

Dr. Goldstein will begin her presentation by giving us a summary of what is
happening in the Microscopy Society of America (MSA) at the national level.
Following this she will give a 30 to 40 minute presentation on imaging muscle.
Power spectra of electron micrographs show that the Z band lattice in mammalian
muscle has at least two structural states related to the contractile state of
the muscle. Cross-sectional projections of relaxed muscle show the small square
pattern, while projections of fixed contracted muscle show a pattern termed the
basket weave. Differing three-dimensional models constructed to visualize the
transition were consistent with the cross-sectional data; therefore, we produced
three-dimensional reconstruction's of Z band lattices from electron tomography
of tilted specimens. The relaxed lattice shows features not imaged in
micrographs of cross or longitudinal sections of muscle. Generating and
analyzing these data lead to challenges in display of these "micro" structures
not found in our "macro" world. Issues of perception and vision are of
demonstrated importance in the display process.


PLEASE MAKE RESERVATIONS BY November 28:
We need to give a "final" head count to the Cherrywood Room.


Dinner and Social Hour: $10.00 per person, $7.50 for students, payable at the
door. Presentation is free for those who come later for the talk only. To
reserve, contact Gib Ahlstrand at (612)625-8249, 625-9728FAX, or:
giba-at-puccini.crl.umn.edu.

Social hour, dinner and the presentation will all be held in the Cherrywood
Room, second floor level of the St. Paul Campus Student Center of the University
of Minnesota. This location will be familiar to some who have attended our
meetings there before. Parking is available nearby.



--

Gib Ahlstrand, MMS Newsletter Editor
Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
495 Borlaug Hall, St. Paul, MN 55108 (612)625-8249
612-625-9728 FAX, giba-at-puccini.crl.umn.edu

"MICROSCOPY & MICROANALYSIS 96" in Minneapolis, Minnesota, Aug.11-15, 1996





From: Ciara Mullan :      mullanc-at-mcmail.cis.mcmaster.ca
Date: Wed, 22 Nov 1995 13:10:13 -0500 (EST)
Subject: Re: etch for FeAl

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Hello

A researcher in my lab requests any information about good (safe)
electropolishing solution and conditions for FeAl thin foils.
Thank you in advance

Ciara Mullan




From: jlibert-at-cpcug.org (John M. Libert)
Date: Wed, 22 Nov 1995 13:12:08 -0500
Subject: Fixing/Staining Protozoans

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My daughter is involved in planning a science fair project in which she will
be assessing various environmental conditions on growth of protozoan
populations. What techniques are used for fixing, staining, or otherwise
preparing these little guys for light microscope examination. If staining is
possible to allow for segmentation from background, I could introduce her to
computer image analysis. Suggestions and/or references on this topic would
be greatly appreciated.

John Libert





From: Alex Titkov :      ALEX-at-bunyip.ph.rmit.edu.au
Date: Thu, 23 Nov 1995 09:02:37 EST-10
Subject: Re: Quantitative EDS of C and O in steels

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Thank you very much everyone answered the question about using EDS for
quantitative analysis of C and O in steels.

---
Alex Titkov
Electron Microscopy Unit
RMIT Applied Physics
GPO Box 2476V
Melbourne VIC 3001 AUSTRALIA
alex-at-bunyip.ph.rmit.edu.au
Voice:(03) 9660 2205
Fax: (03) 9660 3837




From: MSCROGGIE-at-TRENTU.CA
Date: Wed, 22 Nov 1995 20:18:16 -0400 (EDT)
Subject: staining of cadmium in embryonic tissues

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Hello, I am interested if anybody knows of any methods of visualizing
cadmium in tissues treated with cadmium for the light microscope or the
TEM. At the moment I have a method that employs benzothiazolylazonapthol
derivatives(BTAP's) synthesized by Sumi et al. (1979, 1980, 1982) which
allows for the visualization of cadmium in cadmium treated tissues for the
light microscope. Unfortunately the aforesaid papers do not include the
staining protocol for the use of BTAP's. So, if anybody has any experience
visualizing cadmium treated tissues or knows of the protocol for the use of
BTAP's or has any alternatives\suggestions, I would very much appreciate
your feedback. Thank-you very much. MSCROGGIE-at-TRENTU.CA





From: PHOBOS11-at-aol.com
Date: Wed, 22 Nov 1995 21:57:26 -0500
Subject: Re: Parts for carbon coater

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Glenn,

Try a company called Kurt Lesker. They stock common items like Diff heaters
for many brands of pumps.

Best Regards,

Al Coritz




From: H.B. Groen :      h.b.groen-at-phys.rug.nl
Date: Thu, 23 Nov 1995 08:45:26 -0100
Subject: Unsubscribe

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unsubscibe microscopy





From: user image :      schmutzm-at-lear.u-strasbg.fr
Date: Thu, 23 Nov 1995 16:45:04 +0100
Subject: SEM-XL20

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Hello,

I`m looking for a soft wich will allow me to read the *.vct file of the image
from a XL 20 SEM. The image is in TIFF format wich is readable but the
parameters are stored under a Philips format wich I can't read with any of
my softs.

Any help is welcome

______________________________________
SCHMUTZ Marc
IGBMC
BP 163
F67404 ILLKIRCH Cedex
France
Phone 33 88 65 33 32
Fax 33 88 65 32 01
email schmutzm-at-lear.u-strasbg.fr

--------------------------------------




From: Cannon9965-at-aol.com
Date: Thu, 23 Nov 1995 12:48:18 -0500
Subject: Custom microscopy solution?

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Is there anyone who knows an outfit that modifies and/or builds special
custom microscopy equipment? Name and telephone if possible?

M. Davis





From: BARRY PYLE :      umbbp-at-trex.oscs.montana.edu
Date: Thu, 23 Nov 1995 22:40:31 MST
Subject: Unsubscribe

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Please unsubscribe umbbp-at-msu.oscs.montana.edu




From: Stefan.Gunnarsson-at-devbiol.uu.se (Stefan Gunnarsson)
Date: Fri, 24 Nov 1995 08:22:06 +0100
Subject: Re: SEM-XL20

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X-Sender: zoogun-at-strix.udac.uu.se
Message-Id: {v01510100acdb207c463d-at-[130.238.80.10]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Marc Schmutz wrote

} I`m looking for a soft wich will allow me to read the *.vct file of the image
} from a XL 20 SEM. The image is in TIFF format wich is readable but the
} parameters are stored under a Philips format wich I can't read with any of
} my softs.
}

So am I. And I would also be very happy if anyone can give me info on
software that can convert the *.img files of Philips to TIFF so that I can
use the high definition images on other computers than the microscope's. We
have an XL30 but that shouldn't make any difference.

Thanks a lot!

Stefan


..............................................................................

Stefan Gunnarsson
Microscopy Unit,Dept. of Animal Development and Genetics

Uppsala University
Norbyv. 18A, S-75236 UPPSALA, Sweden
e-mail Stefan.Gunnarsson-at-devbiol.uu.se








From: Yves Maniette :      yves-at-giga.sct.ub.es
Date: Fri, 24 Nov 1995 12:02:23 +0000 (GMT)
Subject: Boron carbide mortar: maker?

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Dear all,

I am looking for a small boron carbide mortar, that would be used to
prepare hard samples for TEM, by grinding.

I know that such mortars do exist, but unfortunately I do not remember
the name of the maker(s). Anyone can refresh my memory?

Thank you in advance.

Yves MANIETTE
Universitat de Barcelona
Serveis Cientifico Tecnics
Unitat ESCA TEM
Carrer Luis Sole i Sabaris
E-08028 BARCELONA

Tel +34 3 402 16 95
Fax +34 3 402 13 98





From: jlibert-at-cpcug.org (John M. Libert)
Date: Fri, 24 Nov 1995 09:00:09 -0500
Subject: Re: Custom microscopy solution?

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Bunton Instrument Company, Inc. of Rockville, MD does this.
(301) 762-5115

At 12:48 PM 11/23/95 -0500, Cannon9965-at-aol.com wrote:
} Is there anyone who knows an outfit that modifies and/or builds special
} custom microscopy equipment? Name and telephone if possible?
}
} M. Davis
}
}
}





From: orion-at-infoboard.be (Jean Leclef)
Date: Fri, 24 Nov 1995 16:42:26 +0100
Subject: XL 20 image data decode

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to XL users ...

I now a company in France who has developped a utility for automatic decode of
XL images data and transfer into a Microsoft WORD 6 document. They also have
I think a converter from IMG to TIFF and a high resolution frame grabber for
XL microscopes (get 3000 lines in photo mode instead of 484)

references:

I.C.I. sarl

tel (33) 84 58 02 43
fax (33) 84 54 03 98

they have no Email address but we can transfer them messages if needed.





From: Cannon9965-at-aol.com
Date: Thu, 23 Nov 1995 12:48:18 -0500
Subject: Custom microscopy solution?

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Message-ID: {n1394919306.80617-at-ms.sjdccd.cc.ca.us}

Gatan builds accessory microscopy equipment. Don't know exactly what you
want.
Gatan
6678 Owens Drive
Pleasanton, CA 94566
#162##193#5/463-0200
FAX 415/463-0204
_______________________________________________________________________________

Is there anyone who knows an outfit that modifies and/or builds special
custom microscopy equipment? Name and telephone if possible?

M. Davis


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From: jofu-at-enh.nist.gov (Joe Fu)
Date: Fri, 24 Nov 1995 13:16:27 -0500
Subject: subscribe

Contents Retrieved from Microscopy Listserver Archives
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Hi, please add my name on the list. Thank you.





From: Paul.Fischione-at-internetmci.com
Date: Fri, 24 Nov 1995 20:47:16 -0500
Subject: Electropolishing

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-- [ From: Paul E. Fischione * EMC.Ver #2.3 ] --

Hi Everyone,

In the past few weeks Don Steele and Ciara Mullan have questioned the
electrolytic preparation of Al and FeAl for TEM specimens. I would like to
share some insight. I have found that both of these materials are
conducive to the twin-jet electropolishing technique provided that the
proper electrolyte and polishing conditions are applied.

For Al and some Al alloys I have found success with either 20% perchloric
and 80% ethanol at -30 degress C and about 35 VDC or 20 to 25% Nitric in
Methanol (add liquid nitrogen to the electroyte until it begins to solidify
then begin polishing as soon as it re-liquidifies).

For FeAl either 33% nitric in methanol at -40 to -50 degrees C or 5%
perchloric, 35% Butoxyth, 60% Methanol at -20 degress C and 15 VDC.

When polishing any Al alloy, it is essential that the specimen is removed
immediately from the electropolisher upon perforation and thoroughly rinsed
in ethanol to minimize oxidation.

I hope that this information is helpful. If there are any furbether
questions, please contact me directly. Also, I will be exhibiting at the
MRS meeting in Boston next week and would be happy to address any specimen
preparation questions there.

Best regards,

Paul Fischione
E.A. Fischione Instruments, Inc.
9003 Corporate Circle
Export, PA 15632
Phone 412-325-5444
FAX 412-325-5443
e-mail paul.fischione-at-internetmci.com




From: MicroToday-at-aol.com
Date: Sat, 25 Nov 1995 09:49:54 -0500
Subject: WWW Buyers Guide

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Dear Group -
With this note I would like to advise that we are in the process of
developing a proper, comprehensive "buyers guide" on the Web for
microscopy-related equipment, materials, supplies and services.
In addition to links to other home pages, the system will provide source
information down to the local, international level.
The major challenge is to develop an index which will allow the
convenient selection of ANY microscopy product or service. To this end, we
would appreciate input from any interested party.
From manufacturers and suppliers, we would specifically appreciate a
listing of ALL categories under which they provide equipment, materials,
supplies, and/or services. To them, we will provide drafts of the index as
it is developed. Incidently, we do not plan on "charging" you for basic
listings - but will ask for a MODEST yearly fee for extended listings.
Please contact me directly, not on the listserver, regarding this
matter.
Regards to all,
Don Grimes, Microscopy Today





From: Johari, Dr. Om :      73211.647-at-CompuServe.COM
Date: 26 Nov 95 08:32:18 EST
Subject: Our forthcoming meeting

Contents Retrieved from Microscopy Listserver Archives
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-- [ From: Dr. Om Johari * EMC.Ver #2.0 ] --

Dear Nestor,

Please publicize information in the enclosed.

Thank you.


Om Johari

----

Scanning Microscopy International
Post Office Box 66507, Chicago (A.M.F. O'Hare), IL 60666-0507,
U.S.A.

Telephone: (708) 529-6677 / FAX: (708) 980-6698
E.mail: 73211.647-at-compuserve.com

Scanning Microscopy 1996 meeting
May 11-16, 1996, Bethesda, Maryland (suburb of Washington, DC)

A symposium on: Scanning Probe Microscopies and Related Techniques
for the Biological and Materials Sciences

will be held during the Scanning Microscopy 1996 meeting at the
Hyatt Regency Hotel, Bethesda, [tutorials on May 11 and 12;
scientific programs from May 13 (8:30 AM) till May 16 (noon)] The
symposium organizers are:

Dr. David P. Allison, Oak Ridge National Lab., P.O.Box 2008 - M.S.
6123, Oak Ridge, TN 37831-6123, USA.
(phone: 615 574 6215 / FAX: 615 574 6210 / E.mail:
allisondp-at-ornl.gov);

Prof. Chunli Bai, Institute of Chemistry, Chinese Academy of
Sciences, Beijing, 100080, China
(Phone: 86 10 256 8158 / FAX: 86 10 255 7908 / E.mail:
clbai-at-bepc2.ihep.ac.cn);

Prof. Lawrence A. Bottomley, Georgia Inst. Tech., Chemistry &
Biochemistry Dept., Atlanta, GA 30332-0400
USA (Phone: 404 894 4014 / FAX: 404 894 7452 / E.mail:
lawrence.bottomley-at-chemistry.gatech.edu);

Prof. Masamichi Fujihira, Univ.- Dept. Biomolecular Engg., 4259
Nagatusta, Midori-ku, Yokohama 227, Japan
(phone: 81 22 2152021 / FAX: 81 22 2152020);

Dr. Heinrich J.K. Hoerber, European Molec. Biol. Lab., Meyerhofstr.
1, D-69117 Heidelberg, Germany
(phone: 49 6221 387569 / FAX: 49 6221 387306 / E.mail:
horber-at-embl-heidelberg.de);

Prof. Douglas J. Thomson, Univ. Manitoba, Dept. Electrical &
Computer Engg., Winnipeg MN, R3T 2N2, Canada
(phone: 204 474 8797 / FAX: 204 261 4639 / E.mail:
thomsom-at-ee.umanitoba.ca).

Following up on the past SPM meetings, this symposium will provide
a nice occasion to present novel discoveries as well as reviews of
recent developments in theory, instrumentation, and applications of
scanning tunneling microscopy and related techniques, including
atomic force microscopy, magnetic force microscopy, near field
optical microscopy, etc. Applications of STM and other scanning
probe techniques should emphasize the studies of adsorbates as well
as physical and chemical process at solid surfaces. Topics of
interest include: studies of processes on metal, semiconductor,
and other solid surfaces: imaging of molecules, especially
biomolecules; imaging of cells and other biological structures;
tip-induced effects; etc. This symposium will be held
simultaneously with a number of other symposia on Fundamental
Physics in Microscopy and Microanalysis, Pattern Formation and
Nanoscaled Structures in Thin Film Formation, Scanning Microscopy
and Semiconductors: Metrology and Diagnostics (dealing with
fundamental aspects of material and defect properties,
characterization of surfaces and interfaces in semiconductors,
properties of thin dielectrics, correlation between electrical and
structural properties, new developments in characterization and
imaging techniques); and biological (including microanalysis and
imaging, immunolabelling, radiation effects, dentistry, corrosion
casting, inner ear, bone biology, stones and crystals, etc.); Cells
and Materials; and Food Structure related topics.

For the STM program, all relevant contributions are welcome with
the understanding that a paper presented at this meeting must be
submitted for publication in the quarterly journal Scanning
Microscopy (Instructions for Authors available on request).
Potential contributors are invited to discuss their ideas with one
of the organizers. Suggestions for potential contributors, and
participation in the program are invited. For each contribution,
a completed Letter of Intent form (see over; this form can also be
used to request additional information including a registra-
tion/hotel form, travel support guidelines etc.) should be
submitted as soon as possible (preferably to arrive at Scanning
Microscopy office by December 15, 1995).

The registration fee for the entire 6-day conference (without
subscription to any of the SMI journals' 1996 volumes) is either
$100 (if payment reaches SMI before March 31, 1996) or $120 (from
April 1, 1996); the fees with 1996 subscription to one, two or all
three SMI journals (Scanning Microscopy, Cells and Materials, and
Food Structure) are respectively: $180, $220, or $270 (for US
delivery addresses), or $210, $270, or $340 (for outside US
addresses). The room rates (exclusive of applicable taxes,
currently 15%) at the Hyatt Regency Bethesda (One Bethesda Metro,
Bethesda, MD 20814, USA; phone: 301 657 1234; FAX 301 657 6453)
are: Single (1 person - 1 bed): $100; Double (2 persons - 1 bed)
or Twin (2 persons - 2 beds): $110. Please make room reservations
directly with the hotel.

Scanning Microscopy International will also be sponsoring a
separate international meeting: 15th Pfefferkorn Conference on
Electron Image and Signal Processing (immediately after the
Bethesda Meeting) from May 18-23, 1996 at Albany, New York. The
organizers are: Drs. Peter W. Hawkes (CNRS, Toulouse, France; FAX:
33-62-257999; e.mail: hawkes-at-cict.fr; as its chief organizer), W.
Owen Saxton (Univ. Cambridge, U.K.) and Joachim Frank (NY State
Dept. Health, Albany, NY). Possible contributors should contact
one of the organizers.

For more information about the programs and publications of
Scanning Microscopy, please contact Dr. Om Johari at Scanning
Microscopy International.

-----------
Scanning Microscopy
ISSN: 0891-7035
Volume 9, Number 3, September 1995, Pages 619-926


Table of Contents

Editorial Board
Inside Front Cover

Interactions of Low-Energy Electrons With Atomic and Molecular
Solids (Review paper)
L. Sanche
619

Work Function Dependence of Charge Transfer in
Desorption and Sputtering of Atoms from Surfaces
S. Steuber, P. Nordlander, H. Shao, D.C. Langreth
657

Imaging of Cherenkov and Transition Radiation from Thin Films and
Particles
N. Yamamoto, A. Toda
669

A Reflection Upon the Applicability of Electron Beam Induced
Current (EBIC) as a
Sensitive Microanalytical Technique (ppb range) for Silicon
Materials Research (Tutorial paper)
M. Kittler, W. Seifert
677

Photon Emission Induced by the Scanning Tunneling Microscope
R. Berndt
687

Atomic Force Microscopy of Neuron Networks
A. Cricenti, G. De Stasio, R. Generosi, P. Perfetti, M.T. Ciotti,
D. Mercanti
695

Thermal Stability of Polystyrene Latex Self-Assembled Arrays
Studied by Atomic Force Microscopy
I. Nevernov, C. Nicolini
701

Atomic Force Microscopy of Nucleoprotein Complexes (Review paper)
Y.L. Lyubchenko, B.L. Jacobs, S.M. Lindsay, A. Stasiak
705

Scanning Force Microscopy of Chromatin (Review paper)
W. Fritzsche, J. Vesenka, E. Henderson
729

Consistency in Calibrated Backscattered Electron Images of
Calcified Tissues and Minerals Analyzed in Multiple Imaging
Sessions
E.G. Vajda, J.G. Skedros, R.D. Bloebaum
741

Cross-Linking of Cell Surface Receptors as a Trigger of Cell
Apoptosis and Proliferation
Y.N. Korystov
757

X-Irradiation-Induced Disorganization of Cytoskeletal Filaments and
Cell Contacts in HT29 Cells
Z. Somosy, M. Sass, G. Bognar, J. Kovacs, G.J. Koteles
763

Use of Colloidal Gold and Neutron Activation in
Correlative Microscopic Labeling and Label Quantitation
B.J. Darien, P.A. Sims, K.T. Kruse-Elliott, T.S. Homan,
R.J. Cashwell, A.J. Cooley, R.M. Albrecht
773

Confocal Laser Scanning Microscopic Studies on Alveolar Bone
Remodeling with
Orthodontic Tooth Movement and Retention
H. Yagishita, S. Iwatsubo, T. Aoba
781

Endoarticular Loose Bodies and Calcifications of the Disk of the
Temporomandibular Joint:
Morphological Features and Chemical Composition
C. Piacentini, C. Marchetti, A. Callegari, M. Setti,
G. Bernasconi, U. Baciliero, P. Menghini, C. Brusotti
789

Scanning Electron Microscopy and Energy Dispersive Spectroscopy
Analysis of
Calciotraumatic Lines in Rat Labial Dentin after Acute Exposure to
Strontium Chloride
H. Mishima, T. Sakae, Y. Kozawa
797

The Interaction of Laser Energy with Ureter Tissues in a Long Term
Investigation
U. Stratmann, K. Schaarschmidt, R.R. Lehmann,
A. Heinze, G.H. Willital, E. Unsold
805

In Situ Hybridization and Monoclonal Antibody Analysis of
Plasma Membrane Ca-Pump mRNA and Protein in
Submandibular Glands of Rabbit, Rat and Man
J.L. Borke, A.E. Zaki, D.R. Eisenmann,
S.H. Ashrafi, M.M. Sharawy, S.S. Rahman
817

Comparative Analysis of Patch Lesions in the
Chick Inner Ear Following Acoustic Trauma:
Optical Versus Scanning Electron Microscopy
H.J. Adler, J. Mantooth, Y. Raphael
825

Comparison of DNA Fragmentation and Color Thresholding for
Objective Quantitation of Apoptotic Cells
D. Plymale, D.S. Ng Tang, C.D. Fermin,
D.E. Lewis, D.S. Martin, R.F. Garry
833

Improved Methods for Preserving Macromolecular Structures and
Visualizing Them by Fluorescence and Scanning Electron Microscopy
(Review paper)
P.B. Bell, Jr., B. Safiejko-Mroczka
843

Elicitor-Induced Resistance in Tomato Plants Against Fungal
Pathogens:
Ultrastructure and Cytochemistry of the Induced Response (Review
paper)
N. Benhamou
861

Low Temperature Techniques as a Tool in Plant Pathology (Review
paper)
S. Hippe-Sanwald
881

Impact of Escherichia coli on Urine Citrate and Urease-Induced
Crystallization
A. Edin-Liljegren, H.H. Hedelin, L. Grenabo, S. Pettersson
901

Backscattered Electron Imaging and Energy-Dispersive X-Ray
Microanalysis Studies of Evidence
for Calcium Salt Heterogeneity in Fifteen Gallstones from an
Elderly Human (Review paper)
T. Kodaka, R. Mori, K. Debari, R. Takiguchi, S. Higashi
907

List of Reviewers for Scanning Microscopy Volume 9, number 3, 1995
925-926
Scanning Microscopy International
Post Office Box 66507, Chicago (A.M.F. O'Hare), IL 60666-0507,
U.S.A.
Telephone: (708) 529-6677 / FAX: (708) 980-6698
E.mail: 73211.647-at-compuserve.com

Form below must be completed and returned to remain / get on SMI
mailing lists

Scanning Microscopy Meetings and Publications

Scanning Microscopy, Cells and Materials, and Food Structure 1996
meetings will be from May 11 to 16 at the Hyatt Regency Hotel,
Bethesda, MD (suburb of Washington, D.C.). Programs on the follow-
ing topics are already being planned (*fliers available, list
below): *Scanning Probe Microscopies and Related Techniques for the
Biological and Materials Sciences; Physical sciences programs in:
*Fundamental Physics in Microscopy and Microanalysis, *Pattern
Formation and Nanoscaled Structures in Thin Film Formation,
*Scanning Microscopy and Semiconductors: Metrology and Diagnostics;
Biological programs in: Microanalysis and Imaging, *Im-
munolabelling, *Radiation Effects, Apoptosis, *Dentistry, Corrosion
Casting, Inner Ear, *Bone Biology, *Stones and Crystals, etc.);
*Cells and Materials; and Food Structure related topics. For
participation and contribution, please complete and return the
portion below. Over 400 papers were presented at the Scanning
Microscopy, Cells and Materials, and Food Structure 1995 meeting
from May 6-11, 1995 at Houston. Papers from these programs are
being published in our appropriate journals (Scanning Microscopy /
Cells and Materials / Food Structure) as processed through the
review process.

Papers for publication only in our journals Scanning Microscopy,
Cells and Materials, and Food Structure are invited and can be
offered any time per our Instructions for Authors (printed in each
journal; also, available on request, see below)

Scanning Microscopy International will also be sponsoring a
separate international meeting: 15th Pfefferkorn Conference on
Electron Image and Signal Processing (immediately after the
Bethesda Meeting) from May 18-23, 1996 at Albany, New York. The
organizers are: Drs. Peter W. Hawkes (CNRS, Toulouse, France; FAX:
33-62-257999; E.mail: hawkes-at-cict.fr; as its chief organizer), W.
Owen Saxton (Univ. Cambridge, U.K.) and Joachim Frank (NY State
Dept. Health, Albany, NY). Possible contributors should contact
one of the organizers.
Special Offers on publications: The following special discounts
(expiring Dec. 31, 1995) are applicable to all listed publications
(see our list over) except Scanning Microscopy and its Supplements
for 1992-1996; Food Structure vol. 11-14 (1992-1996) and Cells and
Materials, vol. 2-5 (1992-1996): for orders totaling $100-$299:
Discount 20%; for orders totaling $300-$499: Discount 35%; and
for order totaling more than $500: Discount 50%. See below for
separate discounts on SM Supplements:

Orderers of Scanning Microscopy Supplement 6, 1992 "Signal and
Image Processing in Microscopy and Microanalysis" may obtain
Scanning Microscopy Supplement 2, 1988 on "Image and Signal
Processing in Electron Microscopy" at $20 (for US delivery) or $25
(outside US delivery by sea mail) additional.

Orderers of Scanning Microscopy Supplement 7, 1993 "Physics of
Generation and Detection of Signals Used for Microcharacterization"
may obtain Scanning Microscopy Supplement 4, 1990 on "Fundamental
Electron and Ion Beam Interactions with Solids for Microscopy,
Microanalysis and Microlithography" at $20 (for US delivery) or $25
(outside US delivery by sea mail) additional.

Orderers of Scanning Microscopy Supplement 8, 1994 "Science of
Biological Microanalysis" (to be released in late May) may obtain
three "derived" publications [1. X-Ray Microprobe Analysis of
Chemical Elements in Biology, 1993 (28 papers, 308 pp); 2. The
State of Water in the Cell, 1988 (10 papers, 113 pp); and 3. Basic
Methods in Biological X-ray Microanalysis, 1983 (20 papers, 284
pp)] at a special package price of $100 (for US delivery) or $115
(outside US delivery by sea mail).

---------------------------------------------

__ I wish to present at the Scanning Microscopy 1996 meeting
(tentative title and summary on a separate sheet), please send a
Letter of Intent form.

__ I cannot present, but am likely to attend the 1996 meeting,
please keep me informed and send me:
1996 Registration / Hotel form.

__ I can neither present nor attend; please add/keep my name on
your mailing list.
Send me a mailing list form

Please send: 1996 program fliers (*list programs here):
_________________________________________________________________
_________________________

Instructions for Authors / Major subject index / Table of
Contents for: :
Scanning Microscopy / Cells and Materials / Food
Structure;

Flier on (see titles above and over) Scanning
Microscopy: Supplement 6, 1992; Supplement 7, 1993;
Supplement 8, 1994

Flier on Pfefferkorn Conferences: 1995 on Science of Biological
Specimen Preparation (now over);
1996 on Electron Image and Signal Processing

Name

Title

Organization / Company



Address




City Postal Code

Country

Phone ; FAX
; E.mail
Date




From: jandt-at-msc.cornell.edu
Date: Mon, 27 Nov 1995 08:54:45 -0500 (EST)
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


unsubscribe




From: MicroToday-at-aol.com
Date: Mon, 27 Nov 1995 11:05:26 -0500
Subject: 6th Asia-Pacific Conf on EM

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Does anyone have the correct contact tel./fax numbers for the Sixth
Asia-Pacific Conference on Electron Microscopy, Hong Kong, 1/5 July 1996?
The two sets of numbers that I have seem to be wrong - tel.: 852-609-6845
or 852-559-9973, fax: 852-603-5031 or 852-547-9528.
Thanks for your help!
Regards,
Don Grimes, Microscopy Today




From: trenkler-at-imec.be
Date: Mon, 27 Nov 1995 16:56:22 +0100
Subject: STM/AFM conferences in 1996?

Contents Retrieved from Microscopy Listserver Archives
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Dear readers of this list,

could anybody point me at some conferences taking place in 1996, preferentially
2nd half of the year, dealing with applications of Scanning Probe techniques
in the field of microelectronics (not exclusively, but there should be at least
one or two sessions in this field)?

Thank you in advance

Thomas Trenkler
e-mail: trenkler-at-imec.be




From: kelloes-at-emlab.cb.uga.edu
Date: Mon, 27 Nov 1995 13:14:29 +0000
Subject: Dry Nitrogen dewars for x-ray analysis systems

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Hello To All:

I would like information and opinions on dry nitrogen units for x-ray
analysis systems. Within the next 6 months we will be installing a
new SEM/FEG and we would like to replace our old dewar system.
I have received some comments on the dry system; however, I would like
responses from people who have had actual experience with them and
can tell me the good and bad (if any) of them and how they compare
with the liquid nitrogen dewars. Thank you for your help. Cathy
Kelloes




From: Francisco J. Hernandez :      fjhblasq-at-biomed.icb2.usp.br
Date: Mon, 27 Nov 1995 08:28:06 -0200 (EDT)
Subject: ferritina y peces antarticos (fwd)

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Hola,
Yo he recibido un mensaje de alguien de Chile que
queria cambiar conocimientos sobre observaciones de
ferritina en peces antarticos, pero perdi el mensaje.
Si esta persona lee este comunicado y quiere cambiar
ideas, estoy mui dispuesto a eso y tambien tengo
interes.

Gracias y perdoneme por haber perdido la direccion.
Francisco
fjhblazq-at-spider.usp.br
fjhblasq-at-biomed.icb2.usp.br
_____________________________________________________________________________
Francisco Javier Hernandez Blazquez * Av. Prof. Lineu Prestes, 1524
Departamento de Histologia e Embriologia * 05508-900 Sao Paulo
Instituto de Ciencias Biomedicas * e-mail fjhblazq-at-spider.usp.br
Universidade de Sao Paulo * fjhblasq-at-biomed.icb2.usp.br
______________________________________________________________________________










From: Giles John E Jr :      giles_john_e_jr-at-smtp2.space.honeywell.com
Date: 27 Nov 1995 14:03:15 U
Subject: Image Analysis

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Message-Id: {199511271904.NAA12771-at-Sparc5.Microscopy.Com}

Hello All:

I am looking for information on image analysis programs for the PC. We are in
the process of implementing digital image capture and storage in the SEM lab.
We have a Kevex system with the Feature Analysis and Program Automated Image
analysis programs, but would like to explore using other image analysis
software on the PC (486-66). The majority of our image analysis work involves
counting and sizing metallic particles or feature analysis on porosity of
non-biological specimens.

The ideal software would be more user friendly and versatile than the Kevex
software and hopefully be low cost. I have NIH Image on my Mac, but would like
to get something for the PC as well.

Any opinions and user experiences would be greatly appreciated.

You can reply to me privately (jegiles-at-space.honeywell.com) and I will post a
summary of the replies.

Thanks,

John Giles
Senior Materials Engineer
Honeywell Space Systems




From: gcain-at-digital.net (Gene Cain)
Date: Mon, 27 Nov 1995 12:09:25 -0500
Subject: SEM and Optical Microscope Imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are a Small Disadvantaged Business called Biotex Engineering and
Scientific Services Inc., and we're working on a project for the Navy to
determine if a better system for managing images from electron and optical
microscopes can be developed. To accomplish this, we're contacting various
users to determine how they are presently managing images and related data.
Our goal is to develop a system that will provide fast, efficient, and
affordable total image management. This is a Small Buisness Innovative
Research project and any product developed will be available licenses free
to Government users. Please take the time to provide the following
information which will help ensure the success of our project. Thanks!

Name _____________________________Company____________________________
Loc._______________________________Phone Number_______________________

Would you have an interest in the results of this project? Yes No

What SEMs and Optical microscopes do you have?


How do you presentlly store images? (Film) (Analog: Video Laser Disc)
(Digital: CD-R, Tape, HD)


If using film, would you like to see it eliminated if you could get the same
quality electronically? Yes No If no, why not?


If electronic storage is used, what is the image resolution? (examp: 512 X
512)


What is the flip time (1, 2, 3 sec. or longer) from one image to another and
is it too slow for your needs?) Time: Yes No

Do you network images? Yes No Like to? Yes No


What software applications do you use in image processing and management?


Can your merge and print text and images for reports? Yes No

What improvements would you like to see made in your image management
capabilities?





From: GRAZUL-at-zodiac.rutgers.edu
Date: Tue, 28 Nov 1995 09:07:11 -0500 (EST)
Subject: IMMEDIATE HELP NEEDED!

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HELP!

Does Anyone in the Microscope Community have any information on the
Death of Marcelle Gillott director of the EM lab at the University of
Wisconson Milwaukee??? She got her Ph.D. here at Rutgers from this lab
There is a Funeral service on Staten Island today and we have no idea
where or when. Please send the information ASAP she was a great
person, loved dearly, and we here at Rutgers need to pay our respects

John Grazul
Rutgers University




From: Katherine.S.Connolly-at-Dartmouth.EDU (Katherine S. Connolly)
Date: 28 Nov 95 09:17:27 EST
Subject: NESEM Meeting

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Message-id: {22330872-at-dancer.Dartmouth.EDU}


WHAT: Annual Fall Symposium and Business Meeting of the New England Society
for Electron Microscopy.

WHEN:Wednesday,December 6, 1995 from 1:00 P.M. to 9:00 P.M.

WHERE: Hassenfeld Conference Center, Brandeis University,Waltham,Massachusetts

WHO: Five speakers, Bio and analytical TEM,High Resolution SEM,X-ray
Microanalysis,Patholgy and Fine Arts.

MORE INFO: Laurie Le Tarte
Digital Equiptment Corporation
75 Reed Road,HL02-3/N08
Hudson, Mass 10749
(508)568-7068




From: Richard Lee :      richard_lee-at-QMGATE.ANL.GOV
Date: 28 Nov 1995 11:16:38 -0600
Subject: Vibration isolation

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Subject: Time: 11:12 AM
OFFICE MEMO Vibration isolation Date: 11/28/95

Any suggestions on a quick and easy way to isolate light microscopes from
vibration?





From: GRAZUL-at-zodiac.rutgers.edu
Date: Tue, 28 Nov 1995 11:56:02 -0500 (EST)
Subject: Remembering Marcelle Gillott

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All,

If anyone in the Microscope community wishes to send flowers or
condolences for Marcelle and her family her viewing will be at
Martin Hughs Funeral Home, 998 Bay Street, Statin Island,
New York, (718) 447-0873. There will be a service tomorrow
But I'm not sure where yet.

John Grazul
Rutgers University




From: Rob Schmidt :      RSM-at-eo.ie.philips.nl
Date: Tue, 28 Nov 1995 17:03:49 GMT+0100
Subject: XL : img to tiff conversion

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Dear mr. Gunnarson, Schmutz

On the PC of the XL microscope a DOS utility called MAKTIF.EXE
should be present. With this utility you can convert img files (also
high def) to tiff files.

There is no utility to read the vector information from a XL tiff
file to a *.vct file for later use by the microscope. The vector
information is stored as ASCII text under a private tag in the tiff
file. So you should be able to read that part of the file as simple
ascii text.

If you explain to me in more detail what you mean by "reading" the
vector data, I might be able to help you out a little bit further.

Regards, Rob Schmidt





From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 28 Nov 1995 15:22:04 -0400
Subject: RE-Vib Isolation

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Message-ID: {n1394550250.80965-at-mse.engin.umich.edu}

Subject: Time: 3:11 PM
OFFICE MEMO RE:Vib Isolation Date: 11/28/95

We had reasonably good success isolating a SEM from building traffic
vibrations by setting it on a platform that was suported by four inflated
inner tubes from garden tractor tires. You could probably work out a similar
scheme for a light microscope for very little cost and effort, using
inflatable cushions or smaller inner tubes. Our problem, over the long run,
was that after about six months the inner tubes would begin to leak, and it
was a difficult matter to keep replacing them because the SEM was so heavy
and unwieldy. For a light microscope, which is smaller and lighter, this
should not be such a problem, and so this system might work out quite well
for you. Good luck, W. C. Bigelow (bigelow-at-umich.edu)





From: Richard Thrift :      Richard_Thrift-at-depotech.com
Date: Tue, 28 Nov 1995 12:46:47 -0800
Subject: Vibration isolation -Reply

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Message-Id: {s0bb04bf.056-at-depotech.com}
X-Mailer: Novell GroupWise 4.1

Autoquant (518) 276-2138 sells sheets of 1/2" plexiglass with
"sorbothane" feet (which they say can be stacked for even better
isolation). $200 for a single 24x24" square. I'm curious how well they
work. Also curious how to do a reasonable comparison of different
products: how do you quantitate the effects of vibration in this
application?
What's sorbothane, & can I find it elsewhere?

Richard Thrift

} } } Richard Lee {richard_lee-at-QMGATE.ANL.GOV} 11/28/95 09:16am
} } }
Subject: Time: 11:12 AM
OFFICE MEMO Vibration isolation Date: 11/28/95

Any suggestions on a quick and easy way to isolate light microscopes
from vibration?







From: William Tivol :      tivol-at-wadsworth.org
Date: Tue, 28 Nov 1995 16:07:31 -0500 (EST)
Subject: Re: Vibration isolation

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}
} Any suggestions on a quick and easy way to isolate light microscopes from
} vibration?
}
Dear Richard,
Best is to absorb the vibrations, next best is to mount the LM on a
platform which has no resonances near the frequencies of the vibrations.
A quick method is to mount some styrofoam on a heavy material on another
piece of styrofoam, or use tires or springs instead of the styrofoam. In
either case, the properties of the set-up need to be matched to the prevalent
frequencies. The directionality of the vibrations (if any) is also important.
Good luck.
Yours,
Bill Tivol





From: m.dickson-at-unsw.edu.au (melvyn dickson)
Date: Wed, 29 Nov 1995 10:11:00
Subject: Re: Vibration isolation

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To: microscopy-at-Sparc5.Microscopy.Com

In article {n1394564943.74716-at-qmgate.anl.gov} "Richard Lee" {richard_lee-at-QMGATE.ANL.GOV} writes:
} Date: 28 Nov 1995 11:16:38 -0600
} From: "Richard Lee" {richard_lee-at-QMGATE.ANL.GOV}
} Subject: Vibration isolation

} Subject: Time: 11:12 AM
} OFFICE MEMO Vibration isolation Date: 11/28/95

} Any suggestions on a quick and easy way to isolate light microscopes from
} vibration?

A slab of cement, marble etc. about 24 inches square and 2 inches thick for
mass. The heavier the better. Your local paving store will oblige. Four
tennis balls under the slab for air springs. OR the inner tube of a tire
with the valve relocated to the outside for easy re-inflation is a more
compliant isolation spring.

Mel Dickson





From: philippe.buffat-at-cime.uhd.epfl.ch (Philippe-Andre Buffat)
Date: Wed, 29 Nov 1995 08:29:58 +0100
Subject: used Philips EM300 TEM available

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Hello,

Next autumn we will have to get rid of a Philips EM300 (S+G) TEM to
free-up some space in our lab. It still runs fine (about 700 hrs
beam/year) despite its age (installed in 1972) and is under maintenance
contract with Philips. It is mainly used for teaching. The main limitations
are no EDS nor CBED capabilities. There are a standard stage (=893.5 =C5
resolution, without tilting capability) and a goniometer stage (=89 9=C5
resolution, =B1 60=B0 tilt).

If you are interested, please contact me and we can discuss the details of
its availability (available in autumn 1996).

Thanks!

Philippe-Andre Buffat

__________________________________________________________________
Philippe Buffat Ecole Polytechnique Federale de
Lausanne (EPFL)
Centre Interdepartemental
de Microscopie Electronique
Address: EPFL-CIME, Batiment MX-C, CH-1015 Lausanne, Switzerland
Phone: +41(21)693 29 83 Fax: +41(21)693 44 01 (Central European Time)
E-mail: philippe.buffat-at-cime.uhd.epfl.ch // pabuffat-at-cimesg1.epfl.ch
______________________________ Eudora F2.1 ___________________________






From: philippe.buffat-at-cime.uhd.epfl.ch (Philippe-Andre Buffat)
Date: Wed, 29 Nov 1995 08:32:49 +0100
Subject: used Cambridge S-250 SEM available

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Hello,

We have to get rid of a Cambridge/Leica/LEO S-250 SEM to free-up some
space in our lab. It still runs fine (=895=C5 resolution-at-20keV). It was
installed in 1980 and was is under maintenance contract with Cambridge
until the end of 1993.

If you are interested, please contact me and we can discuss the details of
its availability (available in summer1996).

Thanks!

Philippe-Andre Buffat

__________________________________________________________________
Philippe Buffat Ecole Polytechnique Federale de
Lausanne (EPFL)
Centre Interdepartemental
de Microscopie Electronique
Address: EPFL-CIME, Batiment MX-C, CH-1015 Lausanne, Switzerland
Phone: +41(21)693 29 83 Fax: +41(21)693 44 01 (Central European Time)
E-mail: philippe.buffat-at-cime.uhd.epfl.ch // pabuffat-at-cimesg1.epfl.ch
______________________________ Eudora F2.1 ___________________________






From: philippe.buffat-at-cime.uhd.epfl.ch (Philippe-Andre Buffat)
Date: Wed, 29 Nov 1995 08:29:40 +0100
Subject: used Cambridge S-100 SEM available

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Hello,

Next summer we will have to get rid of a Cambridge/Leica/LEO S-100 SEM
to free-up some space in our lab. It still runs fine, about 1100 hrs
beam/year with students and SE/BSE observation up to =8920'000 X (=895=C5
resolution-at-20keV). It was installed in 1983, it is under maintenance
contract with Cambridge and its electronics has been entirely rejuvenated
(bad contacts) two years ago.

If you are interested, please contact me and we can discuss the details of
its availability (available in summer1996).

Thanks!

Philippe-Andre Buffat

__________________________________________________________________
Philippe Buffat Ecole Polytechnique Federale de
Lausanne (EPFL)
Centre Interdepartemental
de Microscopie Electronique
Address: EPFL-CIME, Batiment MX-C, CH-1015 Lausanne, Switzerland
Phone: +41(21)693 29 83 Fax: +41(21)693 44 01 (Central European Time)
E-mail: philippe.buffat-at-cime.uhd.epfl.ch // pabuffat-at-cimesg1.epfl.ch
______________________________ Eudora F2.1 ___________________________






From: f.lawrence-at-qut.edu.au (Felicity Lawrence)
Date: Wed, 29 Nov 1995 16:19:03 +1000
Subject: Ploem illumination

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Hi everybody,

Can someone please enlighten me as to the meaning of "ploem illumination"?
I have looked up several dictionaries to no avail.

Thanks,
Felicity





From: ScottE57-at-aol.com
Date: Tue, 28 Nov 1995 21:52:08 -0500
Subject: Re: Vibration isolation

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Try A.Q.Sorbothane feet - avaiable at 800-229-0644 for about $60.00 these set
of four hockey puck style "rubber" feet should cut vibration on most scopes.
if not try a Kinetica air table at $3500.00

Scott E. Berman
Advanced Imaging Concepts, Inc.
Princeton, NJ
(908) 274-1877 x26
(908) 274-1974 Fax




From: ScottE57-at-aol.com
Date: Tue, 28 Nov 1995 21:57:53 -0500
Subject: Re: Vibration isolation -Reply

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Richard, you can find Sorbothane or other vibration absorbing products in any
audiophile magazine - a big mail order company that would have it is Audio
Advisor at 800-942-0220 4 sorbothane big feet should run ~$50.00 and for
heavier scopes ther are Sims Navacom silencers for about $75.00. Or try The
Needle Doctor 800-229-0644

Scott E. Berman - Audiophile and Digital Imaging Expert
Advanced Imaging Concepts
Princeton, NJ
(908) 274-1877 x26
(908) 274-1974




From: DDKJoe-at-aol.com
Date: Wed, 29 Nov 1995 07:35:45 -0500
Subject: Re: Vibration isolation

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Richard,

As you can imagine, we have a lot of situations where high mag light
microscopes are close to or mounted on rotating machinery. We've been using
corrugated rubber matting very successfully. The pieces of matting are the
same size as the base of the scope.

Unfortunately I don't know of sources of this material. We bought it so many
years ago that that information is long gone. You might venture down to your
friendly machine shop guys and look through their tool distributor catalogs
(MSC, McMaster-Carr, etc.).

Good luck,

Joe Tabeling
Delaware Diamond Knives
800-222-5143
http://www.ddk.com/ddk




From: Stefan.Gunnarsson-at-devbiol.uu.se (Stefan Gunnarsson)
Date: Wed, 29 Nov 1995 13:27:06 +0100
Subject: Re: Ploem illumination

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} Hi everybody,
}
} Can someone please enlighten me as to the meaning of "ploem illumination"?
} I have looked up several dictionaries to no avail.
}
} Thanks,
} Felicity

Ploem illumination is one type of epi-illumination for fluorescence in
which the excitation filter, dichroic mirror, and barrier filter for a
specific (set of) fluorochrome(s) are mounted together in one cube.
Different cubes are fitted into a revolver in the microscope stand and can
be switched easily. It is the type of epi-illumination used by Leica a.o.

Another question: Felicity, I noticed that you also had posted your
question to a confocal-list. I would be very glad to get subscription
information on that.

Thanks,

Stefan


..............................................................................

Stefan Gunnarsson
Microscopy Unit,Dept. of Animal Development and Genetics

Uppsala University
Norbyv. 18A, S-75236 UPPSALA, Sweden
e-mail Stefan.Gunnarsson-at-devbiol.uu.se








From: SveEn-at-pai.liu.se (Sverker Enestrom)
Date: Wed, 29 Nov 1995 12:28:24 +0100
Subject: Re: Ploem illumination

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} Hi everybody,
}
} Can someone please enlighten me as to the meaning of "ploem illumination"?
} I have looked up several dictionaries to no avail.
}
} Thanks,
} Felicity


J. S. Ploem introduced already 1967 dichromatic mirrors for incident
illumination
(epi-illumination) in fluorescence microscopy. The exciting radiation is
directed
downwards from the light sporce to the object by the dichromatic beam splitter
which acts as a condenser. The fluorescent emission passes back through the
objective
and the dichromatic beam splitter and the barrier filter to the observer.
Ploem, J.S., Z. Wiss. Mikr. 68, 129-142.
Hope this will help you.
Sverker

*********************************************************
Sverker Enestrom M.D., Ph.D.
Department of Pathology
University of Linkoping, Sweden
Phone: +46 13 22 15 20
Fax: +46 13 13 22 57
*********************************************************






From: delannoy-at-welchlink.welch.jhu.edu (Michael Delannoy)
Date: Wed, 29 Nov 1995 09:17:43 -0500
Subject: Confocal vs Decon summary coming

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To the Microscopy List
Earlier this month I posted the Confocal vs Decon question and have
been getting many responses. At the moment we the Leica system (confocal)
on demo, and in Jan we will have the Scanalytics Decon system for two days.
I have been collecting all the responses and will soon create a summary,
together with our demo experiences. Please be patient and keep those opinions
coming.
Thanks to all for your interest,

Mike Delannoy
Microscopy Facility
JHMI
Baltimore, Md





From: Phill :      aarwharn-at-reading.ac.uk
Date: Wed, 29 Nov 1995 16:37:38 +0000 (GMT)
Subject: TEM of Sorghum leaves

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Hello,
I have recently started doing TEM on the infection of sorghum leaves by
the fungus Colletotrichum graminicola. However, I have not had very much
success as of yet because I have had a lot of trouble fixing and
sectioning the leaf tissue. My main problems at the moment are poor
fixation and the resin splitting away from the leaf along the cuticle
when I am sectioning.

I was wondering if anyone had done TEM on sorghum before or if anyone had
any suggestions to how I could get round the problems I mentioned above.

I would be very grateful to recieve any suggestions.

P. Wharton

IACR-Long Ashton Research Station
University of Bristol
UK




From: William Tivol :      tivol-at-wadsworth.org
Date: Wed, 29 Nov 1995 17:26:11 -0500 (EST)
Subject: Re: Vibration isolation -Reply

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} What's sorbothane, & can I find it elsewhere?
}
Dear Richard,
My guess is that it is polyurethane--strictly a guess based on its
property of absorbing energy. Besides it sounds logical.
Yours,
Bill Tivol




From: S_MCGARVEY-at-SAL9K.dnet-at-gmd.fujitsu.com
Date: Wed, 29 Nov 1995 08:43:50 +0800
Subject: SEM VAR's

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Hi all,

We are currently looking into the possibility of utilizing a Video
Archival Retrieval System to store our SEM images rather than
Polaroids.

The VARS that we got the sales pitch on stores the image signals on
a optical memory disk recorder as the primary storage medium. The
disk can store between 36k and 54k different images.

I would like to hear from anyone who has knowledge of these types
of systems.

Pros/Cons/Opinions would be most helpful.


Steve McGarvey
Photolithograhy Process Tech
Fujitsu Microelectronics, Inc.
21015 S.E. Stark Street
Gresham, Oregon, USA
97030-2099

e-mail: steve.mcgarvey-at-gmd.fujitsu.com
Fax: (503) 669-6109
Phone: (503) 669-6118

"There are lies, damned lies, and statistics."




From: Michael Shaffer :      mshaf-at-darkwing.uoregon.edu
Date: Wed, 29 Nov 1995 12:12:36 -0800 (PST)
Subject: Re: SEM/EMPA: Anomalous BSE contrast...why??

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At 08:20 AM 11/29/95 -0700, you wrote:
} John,

I've seen something similar while imaging silicates with our Cameca SX50
at higher KeV (25KeV) rather than what we typically use (15KeV). I had to
question the video amplifier because it didn't make sense. What I should
have tried then, and what I might suggest for you to try is lowering the
beam current for the more intense image signal ...

cheers, shaf

{\/} /\ {\/} /\ {\/} /\ {\/} cognito, ergo zZOooOM {\/} /\ {\/} /\ {\/} /\ {\/}
Michael Shaffer, R.A. - University of Oregon Electron Probe Facility
mshaf-at-oregon.uoregon.edu -or- mshaf-at-darkwing.uoregon.edu
http://darkwing.uoregon.edu/~mshaf/epmahome/





From: Microscopy-request
Date: Tuesday, November 28, 1995 11:03AM
Subject: PC-based integrated wd/ed

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Colleagues,

We are upgrading an AMRAY 1600 tungsten SEM. We will be replacing a TN5600
Be-window EDS and the electronics side of a MicroSpec WDX-2A WDS. We will
be keeping the WD spectrometer itself. We would like to have one integrated

ED/WD system running on an Intel PC using Windows and appropriate
instrument hardware. We have contacted a couple of vendors that say they
can provide acceptable solutions and we will be running samples on their
equipment soon.

Do any of you have experience with the Oxford Link/ISIS system with the
WDX-2A? How about a Kevex integrated system? Any other systems?

Harold J. Crossman
OSRAM SYLVANIA INC.
Lighting Research Center
71 Cherry Hill Dr.
Beverly, MA 01915
(508) 750-1717

e-mail: crossman-at-rd.sylvania.com




From: keller-at-boulder.nist.gov (Bob Keller)
Date: Wed, 29 Nov 1995 08:20:56 -0700
Subject: Re: SEM/EMPA: Anomalous BSE contrast...why??

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John,

Although I'm not certain whether this is the effect you're seeing, it is
somewhat well-documented that electron channeling contrast will exhibit
contrast reversals upon certain changes in energy. The way to check
whether channeling is important here is simply by tilting your sample and
seeing whether the contrast changes, for a constant beam energy. I looked
at your web images, and at first glance, it wasn't apparent to me whether
that was a channeling effect.

Another possibility: is there a chance that at the lower energy, you are
sampling some kind of thin, high Z overlayer in the bright regions
(contamination or another phase or ...) which is for the most part fully
penetrated at 25 kV? For a very thin overlayer, the EDS signal may not see
it well.

Regards,

Bob Keller
NIST
Materials Reliability Division
Boulder, CO






From: Daniel E. Wujek :      34RCKMN-at-CMUVM.CSV.CMICH.EDU
Date: Sun, 19 Nov 1995 16:59:35 -0700 (MST) From: Mark Biedrzycki
Subject: TEM teaching summary

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Thanks to all who responded to my questions concerning teaching TEM
courses. As promised, I am posting this summary of the responses I
received (in a slightly edited fashion). In addition, I have summarized my
findings in this rough table:


How often (times per year) How many students Student Fee?
1 12
No
1 12
No
1
No
0.5 -
No
1 12
yes
1 5
No
1 20-30

1 3-5
No
1 5
No
0.5 8-20
No
1 7
$150
0.5 14
$50
1 10-30
No
1 8
No
1 12
No
1 10-30
No


So, I conclude most of us are teaching the course once a year for 12
students without charging any unusual fee.




} 1. How often do you teach a TEM course?
Once per Fall.

} 2. How many grad/undergrads take it?
About one dozen.

} 3. Do you charge for consumables or beam time?
No, but typically users initiate long term microscopial investigations
that do generate "U revenue."

} 4. Does it make use of a departmental or campus wide facility? If so,
} does it interfere with researchers using the facility?

Departmental. Two lab sessions meet on Thursdays. I suspect a poll of
current users would reveal that this constitutes minimal
interference. We work every day, all hours anyway. Of course lab time is
coordinated with two one hour lectures per week,
and a dedicated microscopy education computer network.

Best regards,

Mark T. Biedrzycki
Computer Networking Laboratory Materials Science and
for Microscopy Education (CNLME) Engineering Department
at the University of Arizona, Tucson



Donald L. Lovett e-mail: lovett-at-trenton.edu
Asst. Professor, Dept. of Biology voice: (609) 771-2876
Trenton State College, NJ 08650-4700 fax: (609) 771-2674



The TEM course is offerred once a year.
We "offer" a certain amount of free beam time to induce interest in the
course. However, students are expected to complete a project, as well, for
which their advisors are expected to pay for the beam time, which is
"discounted" from the normal rate.
The course users have priority over researchers, since the primary function
of a university is to teach.


At Connecticut College, a small liberal arts college, I teach an EM (TEM
and SEM) course every other year. The students are all undergraduate. There
are no fees for the students; my department (i.e. me) runs the microscopes.
I do work very hard on keeping the costs of consumables down, especially
film. We will be adding a digital SEM soon, so I expect a lot of laser
prints rather than expensive Polaroids. Course expenses come out of the
academic supplies budget. I know our situation here is probably quite
different compared to a large university setting.

Page Owen
Connecticut College
Dept. of Botany
tpowe-at-conncoll.edu


Greetings from the University of Tennessee

} 1. How often do you teach a TEM course?

Once a year in the Spring semester
} 2. How many grad/undergrads take it?

Typically 3-4 for the Biological course (i.e Histology) and perhaps 8-10
for the materials science/diffraction course.

} 3. Do you charge for consumables or beam time?

Yes for the Histology course, no for the Materials Science (this anomaly is
the result of an historical accident).

} 4. Does it make use of a departmental or campus wide facility? If so,
} does it interfere with researchers using the facility?

Yes and yes, but the first job of universities is to teach so my research
and every one elses can reasonably be expected to wait once in a while.

David Joy
Professor (Biochemistry, Molecular and Cellular Biology) Director EM Facility
Phone/FAX (423)-974-3642


I've been teaching TEM here at Eastern, a four year undergraduate
institution, for 18 years. The course is offered in the fall semester only.
There are many reasons for this. The main reason is that I have other
teaching responsibilities and no room in my life for two semesters of EM.
The other main reason is that I like to train students in the fall so that
if they really like EM, they can take an independent study under me in the
spring. This also helps me in my research since I don't have any graduate
students.
When teaching the 4 credit EM course, I also require that all of the
students take a one credit "mini" course in Biological Ultrastructure. This
course lasts for 5 weeks at the beginning of the semester. It is also
during those first 5 weeks that the students are undergoing 'basic
training" in the EM course. Around the time the ultrastructure course is
over, the students are just beginning to look at their materials under the
scope and they can now actually identify all of the organelles they learned
about during the previous five weeks.
As far as the number of students, my maximum and minimum is 5. As you
probably know, its hard to tell an administrator that you can only teach a
course to 5 students, especially a couse that is this expensive! Every time
we get a new dean or academic VP, I have to re-explain the course and why I
can only teach 5. By the way, I've never had any trouble getting five for
the course. There is usually a one to two year waiting list.
As I mentioned above, the course is expensive. Not just for supplies, but
also for repair and maintenance. The school has always taken care of
maintenance and repair (its been a struggle on my part to keep costs down)
and a very small amount of supply money. I have, on a few occasions, asked
the students to buy their own film (about $50). Most of the supply money
comes from my research grants (a fact that I do not tend to spread about).

We are a very small department and I am the only one using the TEM, so it
is not a campus-wide facility. I just recently got an SEM and several
people are interested in using it. We will have to work something out.
I hope I have been some help to you. Don't hesitate to contact me if you
need any further information.

Martin A. Levin
Department of Biology
Eastern Connecticut State University
Willimantic, CT 06226
(860) 465-4324




I was involved in a TEM course when I was at Yale University. The facility
I ran was a core TEM/SEM facility for the Biology and Molecular
Biology/Biophysics departments. I was responsible for teaching the Lab
course for the undergrad. course in Cell Biology. The lab consisted of
learning basic skills in LM and TEM. I had one term of 20 to 30 students
per day, five days a week, and
two Graduate TAs per day, who also needed instruction. My goal was to keep
the TAs at least two weeks ahead of the students. It was alot of work for
me, but I really enjoyed it.

I also taught a Summer course to train Graduate students and Post Docs. I
usually got from 7 - 10 individuals per year. As it was my responsibility
to train and supervise anyone in the departments with a demonstrated need
for microscopy, it was easiest to teach a group rather than individuals.

Both courses were extremely rewarding. There were always a few individuals
who mearly wanted to get by, but there were also those with a serious love
for the technique.
Cheers,

Doug Keenee



At the University at Albany, State University of New York, we: 1. Teach a
combined SEM/TEM class once/year. We also offer an advanced class on image
processing.
2. Class size is typically very small (3-5 students). During the late 70s,
early 80's the class was typically 5-10 students. With growing interest in
structural biology/molecular imaging, we hope enrollment will grow to past
levels.
3. No charges beyond tuition. Yet.
4. The class uses equipment at the University-wide EM facility (2 TEMs, 1
SEM), as well as those at the Wadsworth Center, NY State Dept. Health (1
antique SEM, 4 conventional TEMs, and intermediate-voltage TEM, and the
1.2MeV machine). There is plenty of beam time for all.

I'd be interested in your findings.
Regards,
Sam Bowser
--------------------------------
Sam Bowser, Ph.D.
Wadsworth Center
NY State Department of Health
Albany, NY 12201-0509
(518) 473-3856
bowser-at-wadsworth.ph.albany.edu


} 1. How often do you teach a TEM course?
I teach it every semester if there is a demand
for it by 4 or more students. I would prefer to teach it only once a year

} 2. How many grad/undergrads take it?
Enrollment varies. It averages 5 students,
mostly graduate students. I have been trying to train undergrad who will
stay long enough to work on a research project with a professor before
leaving for other endeavors. This has been hindered by the fact that tours
were previously arranged by the upper division courses. Lately we have been
bringing in the freshman biology class on tours and this has sparked new
interest. It is too early to tell if this will produce more students at a
lower grade level. Our tours involve working equipment (i.e., slicing
microtomes, grids in TEM, samples in SEM, thick stained sections on LM,
etc)
} 3. Do you charge for consumables or beam time?
We receive some class supply money and the service contract is covered by
the dept. If we get trained students we can expand our researcher pool by
putting students to work with professors. Researchers do pay beam time and
consumables, but usually are loath to take the time to train or seek out
students for EM.

} 4. Does it make use of a departmental or campus wide facility? If so,
} does it interfere with researchers using the facility?
Most users have been trained here in the Facility and
grumble first as students about researchers time, then as researchers about
student time. Generally not a problem, although this is a reflection in
part of the limited number of users in the Facility. At lab report time,
scope time is a premium, otherwise it is usually available on a walk in
basis.

Dr. Steve Barlow
EM Facility/Biology Dept.
San Diego State University
San Diego CA 92182-4614
phone: (619) 594-4523
fax: (619) 594-5676
email: sbarlow-at-sunstroke.sdsu.edu


Dear Tom; In response to your inquire. We (One other professor and myself)
teach a course called Anatomy 660: Introduction and practice of Electron
microscopy. We teach it every other summer school. It runs for 8 weeks and
meets every afternoon from 1:00 to about 4:00. There is a hour to two hour
lecture and then a demonstration of what subject the lecture was about. It
was designed this way for the student to then start a project and work on
it for the remaining 4 weeks, for a 12 week summer session total, using any
of the techniques they learned about. They supply their own supplies then
during that time. They can use the facility free of charge for that time.
We call in about 6 other "experts" on our campus to teach special subjects.
ie: Immuno, confocal, steriology, etc. We usually have from 8 to 20
students mostly graduate. one or two advanced undergrads. We use the EM
Facility and the hope is to eventually attract new users for the facility.
We need more customers.
Please let me know what your survey shows. If you have more questions
please contact me again. Thanks Grayson Grayson Scott
1300 Univ. Ave
Univ. of Wisconsin
Electron Microscope Facility
Madison, Wisconsin 53706
608-262-2993
Fax 608-262-7306 '''



} 1. How often do you teach a TEM course?
We teach one TEM course in the fall and SEM in spring and summer semesters.
TEM is not as popular as SEM and is certainly more expensive to run. Its
days may be numbered here. SEM is thriving, however.

} 2. How many grad/undergrads take it?
We limit the TEM course to 7 and the SEM to 12. Grad students only. There
is another course on campus for undergrads but this is lecture and
lab/demonstration only. They come to our facility for a 2 hr demo of the
EM's. Specimen prep, interpretation, etc is taught by the lecturer in the
other course. Really interested students then take our course for more
hands on training. This helps to weed out students who are not seriously
interested.

} 3. Do you charge for consumables or beam time?
$150 to take the course. Covers 10 hr scope use. Students must buy all
consumables.

} 4. Does it make use of a departmental or campus wide facility?
Campus wide facility

If so, does it interfere with researchers using the facility? We have two
levels of scopes,older ones for students and newer ones for researchers. We
may stop this in the future as it is too expensive to maintain for the
benefits (cash) derived from students.

Contact me if you need more info.

############################################################################
# John J. Bozzola, Director
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
############################################################################
#


Background: "Comprehensive University" (as distinct from "Research
University", although I find the appelation redundant); 5200 students 4500
of them undergrads. Master's program only, no PhD offered. Roughly 275
biology majors, 12 biology faculty. Research expected of faculty (roughly 1
paper/2 years), involvement of undergrads in faculty research expected.
} } 1. How often do you teach a TEM course?
Every other year, although I will train students in SEM at any point
that their research project may demand it.
222. How many grad/undergrads take it?
Last offering, 14 signed up; nine stuck with it. Two of the nine
were grad students.
} } 3. Do you charge for consumables or beam time?
No. Students taking the course pay a $50 lab fee (lab fees for
other courses in the dept. are $15-$25). Students must buy their own
fine-point foreceps, lab manual, and photographic paper in excess of 50
sheets.
We have no method for charging for beam time; external users
are welcome to use the facility if they pay for plate film or polaroids
(35mm is free).
} } 4. Does it make use of a departmental or campus wide facility? If so,
does it interfere with researchers using the facility? The facility is
departmental. Given the focus on research that involves
undergrads, the course not only doesn't interfere with faculty research, it
enhances it. Service contracts are paid out of the departmental
base budget, which was increased by the necessary amounts as the
microscopes were added.


All-in-all, a pretty cool situation here. HTH
Julian Smith III
Biology
Winthrop University


University of Illinois -at- Urbana-Champaign

I am pleased to respond to your request for information about teaching EM.
It has been a dismal situation here for the past 3 years since the campus
biotechnology center has taken over the operations of the Center for
Electron Microscopy. Biotech refused to support instruction as a part of
their policy (that is now under review and may be reversed). What happened
was that I made an effort to continue some line of instruction, but I do it
without TA's, as an overload assignment without extra compensation, and by
taking money from my research funds to pay the CEM/Biotech. for any
instructional use of equipment and facilities. The single course now
offered is termed: Integrated Electron Microscopy, but has a good deal of
other microscopies also included along with microanalysis. Because it was
just too much for me, there is no lab, but I have fashioned the course to
have weekly demonstration periods. The demos are mostly at the Center for
Electron Microscopy, but include sites at other places on campus as well.
All lab courses have been dropped. The only way students get instruction is
to either do an independent research project with a participating faculty
member (not many of them), or to pay the CEM for a staff member to give
tutorial assistance. For a student with no background this may be about
$1,000 to $2,000. Not many takers.

} 1. How often do you teach a TEM course?

The Integrated EM course is offered once per year--but due to my schedule
may need to go on alternate year basis. (Previously, the courses were
offered both semesters--though not summers--each year.)

} 2. How many grad/undergrads take it?

We have been enrolling about 20-30 students, mostly grads each time, but
this semester we have only 10. One student is an undergrad.

} 3. Do you charge for consumables or beam time?

I do not request a fee from the students since we have demos only. As I
indicated above, I pay for the demo time.

} 4. Does it make use of a departmental or campus wide facility?

Yes--although the facility has become more of a service lab than a
multi-user facility.

If so,
} does it interfere with researchers using the facility?

Not to my knowledge. There is plenty of available time on the equipment.

} If you e-mail me directly I will be happy to post a summary of the
} responses to the list once they are all in.

} Thanks for your input.

You are welcome.

**************************
Richard F. E. Crang
Professor of Plant Biology
University of Illinois
(217) 244-3143
**************************


} 1. How often do you teach a TEM course?
Once per Fall.

} 2. How many grad/undergrads take it?
About one dozen.

} 3. Do you charge for consumables or beam time?
No, but typically users initiate long term microscopial investigations that
do generate "U revenue."

} 4. Does it make use of a departmental or campus wide facility? If so,
} does it interfere with researchers using the facility?
Departmental. Two lab sessions meet on Thursdays. I suspect a poll of
current users would reveal that this constitutes minimal interference. We
work every day, all hours anyway. Of course lab time is coordinated with
two one hour lectures per week, and a dedicated microscopy education
computer network.

Best regards,

Mark T. Biedrzycki
Computer Networking Laboratory Materials Science and
for Microscopy Education (CNLME) Engineering Department
at the University of Arizona, Tucson


1. How often do you teach a TEM course?
==} Basic SEM and TEM undergraduate course taught in fall semester each
year. Advanced TEM taught every other year in the spring semester,
alternating with Advanced SEM and X-ray Microanalysis.

2. How many grad/undergrads take it?
==} 15-30 students take the undergrad SEM/TEM course, aand perhaps half of
these are actually graduate students. About 10 graduate students take the
Advanced TEM course and the Advanced SEM and X-ray Microanalysis course.

3. Do you charge for consumables or beam time?
==} Both are charged to the courses making them rather expensive to run
since each course has approximately 10 different laboratories.

4. Does it make use of a departmental or campus-wide facility? If so,
does it interfere with researchers using the facility?
==} All electron microscopes are in the campus-wide central facility
located in the Dept. of Materials Science and Engineering. All departments
including biology and geology use the instruments in this department. In
addition to our six research electron optics instruments, we maintain three
1970s-vintage microscopes for use by our undergraduate and graduate
students: a Philips EM301 TEM and two ETEC Autoscan SEMs.

CE Lyman

Charles E. Lyman Phone: (610) 758-4249
Prof. of Materials Science and Engineering Fax: (610) 758-4244
Lehigh University E-mail: cel1-at-lehigh.edu
5 East Packer Avenue
Bethlehem, PA 18015-3195

Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility
3 Tucker Hall
University of Missouri
Columbia, MO 65211
(314)-882-4712 (voice)
(314)-882-0123 (fax)






From: Alex Titkov :      ALEX-at-bunyip.ph.rmit.edu.au
Date: Wed, 29 Nov 1995 14:13:18 EST-10
Subject: Summary: Quant EDS of C and O in steels

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A few requests have come to post a summary of responses to a question about
using EDS for quantitative analysis of C and O in steels.
Here it goes ...

DETECTABILITY.
Carbon content for most of steels ranges from 0.05 wt % to 0.95 wt %, and in
some special cases reaches 4 wt%.
Solubility of oxygen in solid iron is very poor, so that the total oxygen
content, with few exceptions, is bellow 100 p.p.m.
Considering the EDS detection limit for light elements is about 0.5 wt%, the
quantitative analysis of both C and O can be a problem. Not less then 1%
(another author gives 5%) can be quantitatively analysed by EDS.

STANDARDS.
At the risk of stating the obvious ! - Reliable , accurate Quantitative
Microprobe Analysis depends very much on having good homogeneous
Standards of known composition .
If you are generating Quantitative data on an element which is present
usually at { 1 wt % in a "matrix " which is usually } 95% Iron you need a
standard which resembles your unknown , what I mean is : you cannot really
use a mineralogical type standard of several percent carbon as a standard
for steels , ZAF effects between Standard and unknown become too marked.
You really need a Steel or Range of Steels as your Standards.

MICROSTRUCTURE, MICROSEGREGATIONS, INCLUSIONS.
When doing E.P.M.A. on Micron sized areas of a steels the underlying
Microstructure becomes important. When probing a steel which has a coarse
Pearlitic phase , your probe spot hits a Cementite lamellae in this region ,
your Carbon counts will skyrocket but will bear no resemblance to the true,
bulk Carbon value of your sample.
Area raster analysis with some beam defocussing, at several sites on your
sample is one way to compensate for Microstructural effects.
Much of the oxygen in steels is tied up in discrete non metallic
inclusions eg.. Silicates , Aluminates . In others there are localised
Oxygen "hot spots" at discrete sites throughout the steel matrix all with the
potential to give misleading results !

OTHER TECHNIQUES.
Because it is problematic to analyse O an C in steels with EDS, a number of
other technique were suggested.

WDS
has better minimum detection limits and better signal discrimination.

BE detector + image analysis.
The oxygen that was dissolved in liquid steel comes out as non-metallic
inclusions. These are commonly aluminates, silicates, and other oxides. So
determining the content of inclusions is an image analysis problem, not one
for X-ray analysis. The area fraction of the inclusions times their oxygen
stoichiometry gives an answer, however, inclusions are generally determined
by standard procedures such as ASTM E 45.
The carbon in high carbon steels is usually in the form of iron carbides. For
example, the optically identifiable phase, "pearlite" is actually a fine
mixture of ferrite with cementite (Fe3C). The carbon concentration in
cementite is stoichiometric at 6.7 wt.%, and need not be analysed. The area
fraction of pearlite by image analysis correlates with the carbon content of
the steel.

EELS
can be useful for light elements.

CALCULATION OF THE DETECTION LIMIT.
Macintosh computer program DTSA--available from the U.S. Natl. Inst. of
Standards and Technology (NIST)-- is to calculate the detection limits and
lots more. DTSA does first-principles computer simulation of EDS spectra
with user-selected experimental variables (detector parameters, electron beam
parameters, physics choices, etc. By simulating the spectra before doing any
experimental work, you can determine the detection sensitivities, assess
spectral interferences and absorption/fluorescence effects, and devise an
optimum strategy for the experimental analysis.

ABSOLUTELY POSITIVE RESPONSE as to the quantitative EDS of C and O in steels
came from Mark T. Biedrzycki (Computer Networking Laboratory, Materials
Science and for Microscopy Education (CNLME), Engineering Department the
University of Arizona, Tucson):

In general, this (EDS analysis of C and O in steels) depends on the
instrument you are using and what type
of detector; the short answer is pretty decent qualitative
and quantitative analyses.
We use Hitachi microscopes and NORAN EDS apparatus to good end
routinely.
If you would like recent publications detailing findings,
please let us know.
=====================================================

Thank you very much all contributed to the topic:

Michael Griffiths
B.H.P. Rod & Bar Products Div
Newcastle.
griffiths.michael.mj-at-bhp.com.au

John J. Friel
Princeton Gamma-Tech
1200 State Rd.
Princeton, NJ 08540
jjf-at-pgt.com

Alan Stone
ASTON Metallurgical Services
73004.1733-at-compuserve.com

Larry Thomas
Battelle, Pacific Northwest Lab
Richland, WA, USA
l_thomas-at-ccmail.pnl.gov

Mark T. Biedrzycki
Computer Networking Laboratory Materials Science and
for Microscopy Education (CNLME) Engineering Department
at the University of Arizona, Tucson
mtb-at-sodium.sem.Arizona.EDU

Ubirajara P. Rodrigues-Filho
Instituto de Qu=EDmica- Universidade Estadual de Campinas
Campinas, SP, Brazil
ubira-at-iqm.unicamp.br

Brian G. Demczyk
demczyk-at-ERXINDY.rl.plh.af.mil


---
Alex Titkov
Electron Microscopy Unit
RMIT Applied Physics
GPO Box 2476V
Melbourne VIC 3001 AUSTRALIA
alex-at-bunyip.ph.rmit.edu.au
Voice:(03) 9660 2205
Fax: (03) 9660 3837




From: dianavd-at-eye.usyd.edu.au (Diana van Driel)
Date: Wed, 29 Nov 1995 13:41:07 +1100
Subject: Re: Vibration isolation

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} Subject: Time: 11:12 AM
} OFFICE MEMO Vibration isolation Date: 11/28/95
}
} Any suggestions on a quick and easy way to isolate light microscopes from
} vibration?

Tennis balls under a wooden frame, on which the LM sits. We have had 6
under a large Zeiss Universal for years and we are right next to a busy
road on the 2nd floor. Works like a dream!


Diana van Driel
Dept Ophthalmology
Sydney University
AUSTRALIA 2006






From: R.G.White-at-sci.monash.edu.au (Rosemary White)
Date: Wed, 29 Nov 1995 12:17:22 +1200
Subject: Re: Vibration isolation

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The Zeiss confocal has some sort of airbag/tyre system for vibration
isolation, like the suggested tractor inner tubes. We've used a bicycle
inner tube about 3/4 filled with water under a heavy terrazzo (fake marble)
slab - seems to work OK.





____________________________________________________________
Rosemary White __ /
Department of Ecology _/ \__/ \
and Evolutionary Biology / \
Monash University / Australia \
Clayton, Victoria 3168 \ ____ /
phone 61-3-9905 5670 \_/ \_*_/
fax 61-3-9905 5613 __
email r.g.white-at-sci.monash.edu.au \/






From: chandler-at-lamar.ColoState.EDU (John Chandler)
Date: Wed, 29 Nov 1995 17:49:47 -0700
Subject: We're looking to upgrade our TEM

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We are looking to upgrade the teaching TEM in our EM Center. If you have a
microscope that you are considering surplussing or trading in, please let
me know.

Also, if you are interested in obtaining a basic, functional scope, let me know.

Trades will be considered.

Please contact me directly, not the microscopy list, for further details.

John
chandler-at-lamar.ColoState.EDU






From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 28 Nov 1995 15:22:04 -0400
Subject: RE-Vib Isolation

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"Wil Bigelow" {Wil_Bigelow-at-mse.engin.umich.edu}
X-Mailer: Mail*Link SMTP-QM 3.0.2

RE} RE-Vib Isolation 11/29/95

Thanks to all for many good suggestions, but stop already! I now have a folder
with over 30 replies to distill down or write a book.

--------------------------------------
Subject: Time: 3:11 PM
OFFICE MEMO RE:Vib Isolation Date: 11/28/95

We had reasonably good success isolating a SEM from building traffic
vibrations by setting it on a platform that was suported by four inflated
inner tubes from garden tractor tires. You could probably work out a similar
scheme for a light microscope for very little cost and effort, using
inflatable cushions or smaller inner tubes. Our problem, over the long run,
was that after about six months the inner tubes would begin to leak, and it
was a difficult matter to keep replacing them because the SEM was so heavy
and unwieldy. For a light microscope, which is smaller and lighter, this
should not be such a problem, and so this system might work out quite well
for you. Good luck, W. C. Bigelow (bigelow-at-umich.edu)


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From: S_MCGARVEY-at-SAL9K.dnet-at-gmd.fujitsu.com
Date: Wed, 29 Nov 1995 17:40:09 +0800
Subject: SEM VARS

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Hi all,

We are currently looking into the possibility of utilizing a Video
Archival Retrieval System to store our SEM images rather than
Polaroids.

The VARS that we got the sales pitch on stores the image signals on
a optical memory disk recorder as the primary storage medium. The
disk can store between 36k and 54k different images.

I would like to hear from anyone who has knowledge of these types
of systems.

Pros/Cons/Opinions would be most helpful.


Steve McGarvey
Photolithograhy Process Tech
Fujitsu Microelectronics, Inc.
21015 S.E. Stark Street
Gresham, Oregon, USA
97030-2099

e-mail: steve.mcgarvey-at-gmd.fujitsu.com
Fax: (503) 669-6109
Phone: (503) 669-6118

"There are lies, damned lies, and statistics."




From: Bray ext. 2740 :      bray-at-HG.ULETH.CA
Date: Wed, 29 Nov 1995 13:03:57 MST
Subject: SEM info on Bryozoan

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Dear Microscopists:
I have a student who has prepared specimens of the Byrozoan "Cristatella
mucedo" for the SEM. Unfortunately a literature search has not turned up
much information on the surface morphology of this specimen or closely
related ones. The references he has obtained so far offer limited light
microscopy images and diagrams only. I was wondering if anyone out there
knows of any references containing SEM micrographs of this organism.
To save listserver bandwidth any responses can be sent to my personal
E-mail address - Bray-at-HG.ULETH.CA
Thanks in advance
Doug Bray




From: Daniel E. Wujek :      34RCKMN-at-CMUVM.CSV.CMICH.EDU
Date: Sun, 19 Nov 1995 16:59:35 -0700 (MST) From: Mark Biedrzycki
Subject: TEM course summary

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Thanks to all who responded to my questions concerning teaching TEM
courses. As promised, I am posting this summary of the responses I
received (in a slightly edited fashion). In addition, I have summarized my
findings in this rough table:


How often (times per year) How many students Student Fee?
1 12
No
1 12
No
1
No
0.5 -
No
1 12
yes
1 5
No
1 20-30

1 3-5
No
1 5
No
0.5 8-20
No
1 7
$150
0.5 14
$50
1 10-30
No
1 8
No
1 12
No
1 10-30
No


So, I conclude most of us are teaching the course once a year for 12
students without charging any unusual fee.




} 1. How often do you teach a TEM course?
Once per Fall.

} 2. How many grad/undergrads take it?
About one dozen.

} 3. Do you charge for consumables or beam time?
No, but typically users initiate long term microscopial investigations
that do generate "U revenue."

} 4. Does it make use of a departmental or campus wide facility? If so,
} does it interfere with researchers using the facility?

Departmental. Two lab sessions meet on Thursdays. I suspect a poll of
current users would reveal that this constitutes minimal
interference. We work every day, all hours anyway. Of course lab time is
coordinated with two one hour lectures per week,
and a dedicated microscopy education computer network.

Best regards,

Mark T. Biedrzycki
Computer Networking Laboratory Materials Science and
for Microscopy Education (CNLME) Engineering Department
at the University of Arizona, Tucson



Donald L. Lovett e-mail: lovett-at-trenton.edu
Asst. Professor, Dept. of Biology voice: (609) 771-2876
Trenton State College, NJ 08650-4700 fax: (609) 771-2674



The TEM course is offerred once a year.
We "offer" a certain amount of free beam time to induce interest in the
course. However, students are expected to complete a project, as well, for
which their advisors are expected to pay for the beam time, which is
"discounted" from the normal rate.
The course users have priority over researchers, since the primary function
of a university is to teach.


At Connecticut College, a small liberal arts college, I teach an EM (TEM
and SEM) course every other year. The students are all undergraduate. There
are no fees for the students; my department (i.e. me) runs the microscopes.
I do work very hard on keeping the costs of consumables down, especially
film. We will be adding a digital SEM soon, so I expect a lot of laser
prints rather than expensive Polaroids. Course expenses come out of the
academic supplies budget. I know our situation here is probably quite
different compared to a large university setting.

Page Owen
Connecticut College
Dept. of Botany
tpowe-at-conncoll.edu


Greetings from the University of Tennessee

} 1. How often do you teach a TEM course?

Once a year in the Spring semester
} 2. How many grad/undergrads take it?

Typically 3-4 for the Biological course (i.e Histology) and perhaps 8-10
for the materials science/diffraction course.

} 3. Do you charge for consumables or beam time?

Yes for the Histology course, no for the Materials Science (this anomaly is
the result of an historical accident).

} 4. Does it make use of a departmental or campus wide facility? If so,
} does it interfere with researchers using the facility?

Yes and yes, but the first job of universities is to teach so my research
and every one elses can reasonably be expected to wait once in a while.

David Joy
Professor (Biochemistry, Molecular and Cellular Biology) Director EM Facility
Phone/FAX (423)-974-3642


I've been teaching TEM here at Eastern, a four year undergraduate
institution, for 18 years. The course is offered in the fall semester only.
There are many reasons for this. The main reason is that I have other
teaching responsibilities and no room in my life for two semesters of EM.
The other main reason is that I like to train students in the fall so that
if they really like EM, they can take an independent study under me in the
spring. This also helps me in my research since I don't have any graduate
students.
When teaching the 4 credit EM course, I also require that all of the
students take a one credit "mini" course in Biological Ultrastructure. This
course lasts for 5 weeks at the beginning of the semester. It is also
during those first 5 weeks that the students are undergoing 'basic
training" in the EM course. Around the time the ultrastructure course is
over, the students are just beginning to look at their materials under the
scope and they can now actually identify all of the organelles they learned
about during the previous five weeks.
As far as the number of students, my maximum and minimum is 5. As you
probably know, its hard to tell an administrator that you can only teach a
course to 5 students, especially a couse that is this expensive! Every time
we get a new dean or academic VP, I have to re-explain the course and why I
can only teach 5. By the way, I've never had any trouble getting five for
the course. There is usually a one to two year waiting list.
As I mentioned above, the course is expensive. Not just for supplies, but
also for repair and maintenance. The school has always taken care of
maintenance and repair (its been a struggle on my part to keep costs down)
and a very small amount of supply money. I have, on a few occasions, asked
the students to buy their own film (about $50). Most of the supply money
comes from my research grants (a fact that I do not tend to spread about).

We are a very small department and I am the only one using the TEM, so it
is not a campus-wide facility. I just recently got an SEM and several
people are interested in using it. We will have to work something out.
I hope I have been some help to you. Don't hesitate to contact me if you
need any further information.

Martin A. Levin
Department of Biology
Eastern Connecticut State University
Willimantic, CT 06226
(860) 465-4324




I was involved in a TEM course when I was at Yale University. The facility
I ran was a core TEM/SEM facility for the Biology and Molecular
Biology/Biophysics departments. I was responsible for teaching the Lab
course for the undergrad. course in Cell Biology. The lab consisted of
learning basic skills in LM and TEM. I had one term of 20 to 30 students
per day, five days a week, and
two Graduate TAs per day, who also needed instruction. My goal was to keep
the TAs at least two weeks ahead of the students. It was alot of work for
me, but I really enjoyed it.

I also taught a Summer course to train Graduate students and Post Docs. I
usually got from 7 - 10 individuals per year. As it was my responsibility
to train and supervise anyone in the departments with a demonstrated need
for microscopy, it was easiest to teach a group rather than individuals.

Both courses were extremely rewarding. There were always a few individuals
who mearly wanted to get by, but there were also those with a serious love
for the technique.
Cheers,

Doug Keenee



At the University at Albany, State University of New York, we: 1. Teach a
combined SEM/TEM class once/year. We also offer an advanced class on image
processing.
2. Class size is typically very small (3-5 students). During the late 70s,
early 80's the class was typically 5-10 students. With growing interest in
structural biology/molecular imaging, we hope enrollment will grow to past
levels.
3. No charges beyond tuition. Yet.
4. The class uses equipment at the University-wide EM facility (2 TEMs, 1
SEM), as well as those at the Wadsworth Center, NY State Dept. Health (1
antique SEM, 4 conventional TEMs, and intermediate-voltage TEM, and the
1.2MeV machine). There is plenty of beam time for all.

I'd be interested in your findings.
Regards,
Sam Bowser
--------------------------------
Sam Bowser, Ph.D.
Wadsworth Center
NY State Department of Health
Albany, NY 12201-0509
(518) 473-3856
bowser-at-wadsworth.ph.albany.edu


} 1. How often do you teach a TEM course?
I teach it every semester if there is a demand
for it by 4 or more students. I would prefer to teach it only once a year

} 2. How many grad/undergrads take it?
Enrollment varies. It averages 5 students,
mostly graduate students. I have been trying to train undergrad who will
stay long enough to work on a research project with a professor before
leaving for other endeavors. This has been hindered by the fact that tours
were previously arranged by the upper division courses. Lately we have been
bringing in the freshman biology class on tours and this has sparked new
interest. It is too early to tell if this will produce more students at a
lower grade level. Our tours involve working equipment (i.e., slicing
microtomes, grids in TEM, samples in SEM, thick stained sections on LM,
etc)
} 3. Do you charge for consumables or beam time?
We receive some class supply money and the service contract is covered by
the dept. If we get trained students we can expand our researcher pool by
putting students to work with professors. Researchers do pay beam time and
consumables, but usually are loath to take the time to train or seek out
students for EM.

} 4. Does it make use of a departmental or campus wide facility? If so,
} does it interfere with researchers using the facility?
Most users have been trained here in the Facility and
grumble first as students about researchers time, then as researchers about
student time. Generally not a problem, although this is a reflection in
part of the limited number of users in the Facility. At lab report time,
scope time is a premium, otherwise it is usually available on a walk in
basis.

Dr. Steve Barlow
EM Facility/Biology Dept.
San Diego State University
San Diego CA 92182-4614
phone: (619) 594-4523
fax: (619) 594-5676
email: sbarlow-at-sunstroke.sdsu.edu


Dear Tom; In response to your inquire. We (One other professor and myself)
teach a course called Anatomy 660: Introduction and practice of Electron
microscopy. We teach it every other summer school. It runs for 8 weeks and
meets every afternoon from 1:00 to about 4:00. There is a hour to two hour
lecture and then a demonstration of what subject the lecture was about. It
was designed this way for the student to then start a project and work on
it for the remaining 4 weeks, for a 12 week summer session total, using any
of the techniques they learned about. They supply their own supplies then
during that time. They can use the facility free of charge for that time.
We call in about 6 other "experts" on our campus to teach special subjects.
ie: Immuno, confocal, steriology, etc. We usually have from 8 to 20
students mostly graduate. one or two advanced undergrads. We use the EM
Facility and the hope is to eventually attract new users for the facility.
We need more customers.
Please let me know what your survey shows. If you have more questions
please contact me again. Thanks Grayson Grayson Scott
1300 Univ. Ave
Univ. of Wisconsin
Electron Microscope Facility
Madison, Wisconsin 53706
608-262-2993
Fax 608-262-7306 '''



} 1. How often do you teach a TEM course?
We teach one TEM course in the fall and SEM in spring and summer semesters.
TEM is not as popular as SEM and is certainly more expensive to run. Its
days may be numbered here. SEM is thriving, however.

} 2. How many grad/undergrads take it?
We limit the TEM course to 7 and the SEM to 12. Grad students only. There
is another course on campus for undergrads but this is lecture and
lab/demonstration only. They come to our facility for a 2 hr demo of the
EM's. Specimen prep, interpretation, etc is taught by the lecturer in the
other course. Really interested students then take our course for more
hands on training. This helps to weed out students who are not seriously
interested.

} 3. Do you charge for consumables or beam time?
$150 to take the course. Covers 10 hr scope use. Students must buy all
consumables.

} 4. Does it make use of a departmental or campus wide facility?
Campus wide facility

If so, does it interfere with researchers using the facility? We have two
levels of scopes,older ones for students and newer ones for researchers. We
may stop this in the future as it is too expensive to maintain for the
benefits (cash) derived from students.

Contact me if you need more info.

############################################################################
# John J. Bozzola, Director
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
############################################################################
#


Background: "Comprehensive University" (as distinct from "Research
University", although I find the appelation redundant); 5200 students 4500
of them undergrads. Master's program only, no PhD offered. Roughly 275
biology majors, 12 biology faculty. Research expected of faculty (roughly 1
paper/2 years), involvement of undergrads in faculty research expected.
} } 1. How often do you teach a TEM course?
Every other year, although I will train students in SEM at any point
that their research project may demand it.
222. How many grad/undergrads take it?
Last offering, 14 signed up; nine stuck with it. Two of the nine
were grad students.
} } 3. Do you charge for consumables or beam time?
No. Students taking the course pay a $50 lab fee (lab fees for
other courses in the dept. are $15-$25). Students must buy their own
fine-point foreceps, lab manual, and photographic paper in excess of 50
sheets.
We have no method for charging for beam time; external users
are welcome to use the facility if they pay for plate film or polaroids
(35mm is free).
} } 4. Does it make use of a departmental or campus wide facility? If so,
does it interfere with researchers using the facility? The facility is
departmental. Given the focus on research that involves
undergrads, the course not only doesn't interfere with faculty research, it
enhances it. Service contracts are paid out of the departmental
base budget, which was increased by the necessary amounts as the
microscopes were added.


All-in-all, a pretty cool situation here. HTH
Julian Smith III
Biology
Winthrop University


University of Illinois -at- Urbana-Champaign

I am pleased to respond to your request for information about teaching EM.
It has been a dismal situation here for the past 3 years since the campus
biotechnology center has taken over the operations of the Center for
Electron Microscopy. Biotech refused to support instruction as a part of
their policy (that is now under review and may be reversed). What happened
was that I made an effort to continue some line of instruction, but I do it
without TA's, as an overload assignment without extra compensation, and by
taking money from my research funds to pay the CEM/Biotech. for any
instructional use of equipment and facilities. The single course now
offered is termed: Integrated Electron Microscopy, but has a good deal of
other microscopies also included along with microanalysis. Because it was
just too much for me, there is no lab, but I have fashioned the course to
have weekly demonstration periods. The demos are mostly at the Center for
Electron Microscopy, but include sites at other places on campus as well.
All lab courses have been dropped. The only way students get instruction is
to either do an independent research project with a participating faculty
member (not many of them), or to pay the CEM for a staff member to give
tutorial assistance. For a student with no background this may be about
$1,000 to $2,000. Not many takers.

} 1. How often do you teach a TEM course?

The Integrated EM course is offered once per year--but due to my schedule
may need to go on alternate year basis. (Previously, the courses were
offered both semesters--though not summers--each year.)

} 2. How many grad/undergrads take it?

We have been enrolling about 20-30 students, mostly grads each time, but
this semester we have only 10. One student is an undergrad.

} 3. Do you charge for consumables or beam time?

I do not request a fee from the students since we have demos only. As I
indicated above, I pay for the demo time.

} 4. Does it make use of a departmental or campus wide facility?

Yes--although the facility has become more of a service lab than a
multi-user facility.

If so,
} does it interfere with researchers using the facility?

Not to my knowledge. There is plenty of available time on the equipment.

} If you e-mail me directly I will be happy to post a summary of the
} responses to the list once they are all in.

} Thanks for your input.

You are welcome.

**************************
Richard F. E. Crang
Professor of Plant Biology
University of Illinois
(217) 244-3143
**************************


} 1. How often do you teach a TEM course?
Once per Fall.

} 2. How many grad/undergrads take it?
About one dozen.

} 3. Do you charge for consumables or beam time?
No, but typically users initiate long term microscopial investigations that
do generate "U revenue."

} 4. Does it make use of a departmental or campus wide facility? If so,
} does it interfere with researchers using the facility?
Departmental. Two lab sessions meet on Thursdays. I suspect a poll of
current users would reveal that this constitutes minimal interference. We
work every day, all hours anyway. Of course lab time is coordinated with
two one hour lectures per week, and a dedicated microscopy education
computer network.

Best regards,

Mark T. Biedrzycki
Computer Networking Laboratory Materials Science and
for Microscopy Education (CNLME) Engineering Department
at the University of Arizona, Tucson


1. How often do you teach a TEM course?
==} Basic SEM and TEM undergraduate course taught in fall semester each
year. Advanced TEM taught every other year in the spring semester,
alternating with Advanced SEM and X-ray Microanalysis.

2. How many grad/undergrads take it?
==} 15-30 students take the undergrad SEM/TEM course, aand perhaps half of
these are actually graduate students. About 10 graduate students take the
Advanced TEM course and the Advanced SEM and X-ray Microanalysis course.

3. Do you charge for consumables or beam time?
==} Both are charged to the courses making them rather expensive to run
since each course has approximately 10 different laboratories.

4. Does it make use of a departmental or campus-wide facility? If so,
does it interfere with researchers using the facility?
==} All electron microscopes are in the campus-wide central facility
located in the Dept. of Materials Science and Engineering. All departments
including biology and geology use the instruments in this department. In
addition to our six research electron optics instruments, we maintain three
1970s-vintage microscopes for use by our undergraduate and graduate
students: a Philips EM301 TEM and two ETEC Autoscan SEMs.

CE Lyman

Charles E. Lyman Phone: (610) 758-4249
Prof. of Materials Science and Engineering Fax: (610) 758-4244
Lehigh University E-mail: cel1-at-lehigh.edu
5 East Packer Avenue
Bethlehem, PA 18015-3195

Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility
3 Tucker Hall
University of Missouri
Columbia, MO 65211
(314)-882-4712 (voice)
(314)-882-0123 (fax)






From: nano-at-ns1.LIHTI.ORG (Nanoprobes Inc.)
Date: Thu, 30 Nov 1995 05:53:41 -0500
Subject: Re: SEM immunolabeling

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Message-Id: {199511301051.FAA02400-at-lihti.org}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Probably the most comprehensive general introduction to immunogold
labeling, since it covers most applications, is vol. 1 and 2 from the
series "Colloidal Gold: Principles, Methods and Applications" edited by M.
A. Hayat (Academic Press, San Diego, CA; 1989 (Vol. 1 and 2) and 1991 (Vol.
3).

Richard D. Powell

Nanoprobes, Incorporated.
URL: http://www.lihti.org/research/ecodev/incubten/nano/home.html

*****************************************************************

} I would like to have suggestions for labeling intracellular antigen with
} gold conjugated antibody. References, techniques etc.






From: nano-at-ns1.LIHTI.ORG (Nanoprobes Inc.)
Date: Thu, 30 Nov 1995 05:53:41 -0500
Subject: Re: SEM immunolabeling

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Message-Id: {199511301051.FAA02400-at-lihti.org}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Probably the most comprehensive general introduction to immunogold
labeling, since it covers most applications, is vol. 1 and 2 from the
series "Colloidal Gold: Principles, Methods and Applications" edited by M.
A. Hayat (Academic Press, San Diego, CA; 1989 (Vol. 1 and 2) and 1991 (Vol.
3).

Richard D. Powell

Nanoprobes, Incorporated.
URL: http://www.lihti.org/research/ecodev/incubten/nano/home.html

*****************************************************************

} I would like to have suggestions for labeling intracellular antigen with
} gold conjugated antibody. References, techniques etc.






From: OptoMech-at-aol.com
Date: Thu, 30 Nov 1995 08:35:37 -0500
Subject: Re: Vibration isolation

Contents Retrieved from Microscopy Listserver Archives
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Mouse pads, tennis balls cut in half, heavy metal plates with neoprene feet.

M. Coombs
Opto-Mechanical Consultants
2102 Riding Crop Way
Baltimore, MD 21244
410-944-1721 phone/fax




From: treese-at-mbl.edu (Tom Reese)
Date: Thu, 30 Nov 1995 14:22:22 -0500
Subject: position available

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Senior technician in biological light (confocal) and electron microscopy
including thin sectioning, various replica techniques, and maintainance of
extensive array of preparative equipment. Photography and image processing
are not part of job. Annual travel to satellite laboratories necessary.
Inquire privately to: treese-at-mbl.edu (Tom Reese)






From: paulc-at-arms.gps.caltech.edu (Paul K. Carpenter)
Date: Thu, 30 Nov 1995 10:10:59 -0800
Subject: Re: SEM/EMPA: Anomalous BSE contrast...why??

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Message-Id: {v02110101ace39f718f9e-at-[131.215.67.110]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

On the continuing discussion of "strange" BSE contrast, especially at low KV:

There is a slight energy dependence (i.e. accelerating voltage) of the
backscattered-electron coefficient (eta) as a function of atomic number at
15-30 KV. For example, see figures 3.13 through 3.15 in the Goldstein text
(Scanning Electron Microscopy and X-ray Microanalysis, but hey everybody
has this right?).

As you know, the atomic number dependence of eta is strongest over the low
atomic number range, so that is where BSE contrast is greatest. It has
also been pointed out (MAS talks in New Orleans or Breckenridge) that eta
may show strong KV dependence as well, especially at lower KV. Compare the
values of eta for 5 KV with those for the other values; they are the
highest for low atomic number and the lowest at higher atomic number
(figure 3.15). There also appear to be some values of eta that exhibit
contrast reversal (see fig 3.14). So you could in fact see contrast
reversal when comparing BSE intensities between different accelerating
voltages.

As others have suggested, the low KV regime is affected by contamination,
and since we are really talking about surface analysis when at 5 KV, it is
certainly possible to see contrast reversal due to the competing effects of
surface properties coupled with the (as yet imperfectly understood) effects
of eta dependence on KV.

I'm sorry I don't have the original post, but I recall we are talking about
alloys in the B-Si-Mo system. Despite the best of sample prep methods, it
may be that there is surface oxidation (or some other reaction product)
that is making itself known to you at 5 KV that was not as important at
15-20 KV.

It certainly is an interesting aspect of microanalysis.

Paul Carpenter



+----------------------------------------------------------------------+
| Paul K. Carpenter paulc-at-arms.gps.caltech.edu |
| Division Analytical Facility Department of Geology 170-25 |
| California Institute of Technology Pasadena CA 91125 |
| 818-395-6126 (X-ray Lab) 818-568-0935 (FAX, Departmental) |
+----------------------------------------------------------------------+






From: Owen P. Mills :      opmills-at-mtu.edu
Date: Thu, 30 Nov 1995 12:49:35 -0500
Subject: Sample prep - polymer nanoparticles

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Hello,

This is a summary of the responses I received to my original request
(included below) for information on polymer nanoparticle sample prep. =20

Thanks to all who responded!

Owen

} =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=
=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=
=3D=3D=3D=3D=3D=3D=3D=3D=3D
}
} Hello;
}
} I'm working with a group that wishes to make morphological measurements on
polymer nanoparticles using electron microscopy (SEM or TEM). The
nanoparticles will likely be polyvinylpyridine, polycyanoacrylate, possibly
polymethylmethacrylate. The nanoparticles should be in the range of
100-200nm, but they may well be bigger. They would like to see the whole
range of particles and be able to report average particle diameter and the
whole range of particle sizes (size polydispersity). =20
}
} Previous attempts, by placing drops of particles suspended a solvent on a
SEM mount or TEM grid, have resulted in "clumps" of particles that are
difficult to measure. =20
}
} Can anyone recommend a prodecure that might work for this situation. I
have heard of (but am unfamiliar with) aerosol procedures, will that work?
}
} Also, can anyone recommend a few useful general references on electron
microscopy of polymers. I have "Polymer Microscopy" by L. Sawyer & D. Grubb
on order now.
}
} Thanks.
}
} Owen Mills
} =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=
=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=
=3D=3D=3D=3D=3D=3D=3D=3D=3D
} } The nanoparticles should be in the range of
} } 100-200nm, but they may well be bigger. They would like to see the whole
} } range of particles and be able to report average particle diameter and the
} } whole range of particle sizes (size polydispersity). =20
}
} I have observed some nanoparticles in TEM to obtain the highest contrast=
for
} automatic image analysis. In SEM the measurements are not as easy as in=
TEM.
}
} }
} } Previous attempts, by placing drops of particles suspended a solvent on a
} } SEM mount or TEM grid, have resulted in "clumps" of particles that are
} } difficult to measure.=20
}
} We have observed the same phenomena, too. If your support film can stand=
it,
} try using e.g. ethanol as the liquid for the suspension. In our case it
} helps to keep the particles better in a "monolayer" but we have had
} difficulties with the TEM-support films - they trend to break due to=
ethanol
} (they stand nicely water).
} =20
} }
} } Can anyone recommend a prodecure that might work for this situation. I=
have
} } heard of (but am unfamiliar with) aerosol procedures, will that work?
}
} Here I cannot help you but I know that e.g. virus particles, DNA, etc. can
} be spread by aerosol blowing. Try to find the method in e.g. "Practical
} methods in electron microscopy".
} }
}
} Let me hear about your success.
}
}
} Best wishes,
}
} Jouko M=E4ki
} Jouko K. M=E4ki, Ph.D., Laboratory Manager
} University of Turku, Laboratory of Electron Microscopy
} Kiinamyllynkatu 10 FIN-20520 TURKU FINLAND
} Tel: +358 21 633 7318 GSM: +358 40 505 2521 FAX: +358 21 633 7380
}
} =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=
=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=
=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D
} 1. use some surfactant (Triton-X?) in low concentration in solution with=
the
} particles to help reduce clumping - the clumping is basically a surface
} tension problem and any ting you can do to reduce the surface tension will
} help.=20
}
} 2. Try serial dilutions of the particles.
}
} 3. Disperse them onto 12mm glass cover slips for SEM or carbon films for=
TEM
} - you might try altering the surface characteristics of these substrates =
to
} help dispersion (glow discharge, chemical treatment, etc.)
}
} 4. Atomization will help but for such small particles you will probably=
just
} make micro clumps but it is easy to do so worth trying.=20
}
} Good Luck -
}
} Bill Miller
}
} =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=
=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=
=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D
A paper, "Quantification of particle sizes with metal replication under=
standard
} freeze-etching conditions: a gold ball standard for calibrating shadow=
widths
} was used to measure freeze-etched globular proteins" Microscopy research=
and
} Technique 32 (1995) 312-329.] was just published that employs 45deg.
} unidirectional Pt-C replication to measure particles. This paper will give=
you
} insight into the problems of replication and how to avoid the pitfalls----=
at
} the end of the paper is a vertical replication method which (Fig. 17b) is=
the
} most powerful and reliable of the replication methods. This method employs=
a
} small metal coating correction to achieve the actual particle size (see ref=
in
} paper). This method would allow you to measure the asymmetry of the=
particles
} as well.=20
} Another method which is more readily available to you but can not help
} you in the 1-2 nm size range is a negative staining technique. For this=
method
} you would need to make up a 2% solution of Uranyl acetate in a tin foil=
covered
} bottle (light sensitive) and filter thru a 0.22 micron filter before use!=
You
} would need 10-12 nm thick carbon films covered 300 mesh grids. Use a 10
} microliter drop of your particles in ethanol, propyl or isoppropyl alcohol=
or
} some solvent compatible with water ---- and put it on the carbon film for a
} minute. Remove the excess alcohol from the edge of the grid with torn=
Whatman
} #1 filter paper. Apply 5-10 microliters of stain for a minute and remove=
with
} the filter paper method and repeat the procedure! Dry the grids from the=
edge
} with torn Whatman #1 filter paper and then look at your grids in the TEM.=
You
} will want to measure the white particles you see surrounded by dark stain. =
=20
}
} George C. Ruben
} Dept. Biological Sciences
} Dartmouth College
} Hanover, NH 03755
} 603-646-2144
} 603-646-1347 fax
} George.C.Ruben-at-Dartmouth.EDU
}
} =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=
=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=
=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D
} We do this type of analysis by cryoTEM of vitrified thin liquid films of
} dispersed particles. We collect the images on a slow-scan CCD camera using
} Gatan's DigitalMicrograph and do image analysis using NIH Image.
} We compute the size distributions using a statistical package
} called RS/1 (on the VAX or Sun) and fit the data to a lognormal
} distribution with up to three modes.
}
} We have tried aerosol methods and have not been satisfied with the
} fraction of isolated particles. We do MUCH better with cryoTEM.
} The good news is that one can automatically
} select only isolated spheroidal particles from the images
} containing some overlapping particles by calculating
} the circularity of each feature (C=3D4*pi*area/perimeter**2). For such=
particles
} we only keep features with 0.90 } C } 1.05. Verify this yourself by
} measuring model images of agglomerated circles. By the way, it took me
} almost a year to develop this capability to the point that I convinced
} myself that I had done everything correctly. It was not a trivial
} project.
}
} minter-at-Kodak.COM (John Minter)
} =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=
=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=
=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D
} I don't know if my suggestion will be of any use but I guess it's worth=20
} sharing. I work with nanometre size ceramic particles and to get them in=
=20
} the TEM I disperse them in methanol ultrasonically and then pick them up on=
=20
} a carbon film supported on a copper grid. I then place the grid on some=20
} tissue or filter paper which quickly wicks away the methanol. This usually=
=20
} gives good results if the particle suspension is thin enough. We usually=
=20
} see some particle overlaps but there are usually enough free particles to=
do=20
} some good TEM.
} Do you use ultrasonic agitation to break up any particle agglomerates (we=
=20
} need to as ceramic systems are often very prone to agglomeration)? What=20
} liquid do you use to disperse the particles in? Have you tried all the=20
} ideas suggested above already?
}
} I hope this is of some help to you
}
} Best wishes
}
}
} Ian MacLaren,
} IRC in Materials for High Telephone: 0121 414 3447 =20
} Performance Applications, FAX: 0121 414 3441
} The University of Birmingham, email: I.MacLaren-at-bham.ac.uk
} Birmingham B15 2TT, =20
} England
}
=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=
=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=
=3D=3D=3D
} In my prior life as a chemical engineer, I was responsible for a colloidal
} silica unit. Whenever questions were raised about particle size
} distribution, we would walk a sample over to our friendly electron
} microscopist for analysis.
}
} His secret was to perform a series of dilutions to very dilute levels of=
Si.
} My recollection is that he did a series of three or four 1:100 dilutions
} before drying a drop on a coated grid. This created a single particle=
thick
} film of these normally sticky SiOx particles.
}
} We were routinely dealing with discrete particle sizes from 6 to 150 nm.
}
}
} Good luck,
} Joe Tabeling
} Delaware Diamond Knives
} 800-222-5143
}
} =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=
=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=
=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D
} 1) You may have to try to sonicate the suspension right before=20
} putting it on the grid or SEM stub.
} =09
} 2) If you can get hold of the dry sample (before they put it in=20
} the solvent) and if it looks powdery then you can just pick a little bit=20
} up with the tip of a pastuer pipette and gently blow it on the grid or=20
} glass cover slid for SEM.
}
}
} Have fun and good luck.
}
} Yew Meng Heng
}
} E.M. Facility=20
} Mcmaster University Medical Centre
} Hamilton, Ont., Canada
} =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=
=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=
=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D
} -- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --
}
} On Nov. 7, Owen P. Mills wrote inquiring about the preparation of
} polymer particles in the range of 100-200 nm.
}
} We do a large volume of these kinds of samples in our laboratory:
}
} 1] If they are clumping, then probably the concentration of the
} suspension is too high and you need a higher dilution by at least one
} and maybe two or three orders of magnitude.
}
} 2] You want to use an "aerosol" type "duster" valve that incorporates
} a capillary tube to disperse the now highly diluted suspension. The
} best (but certainly not the only) one I know of is made by Ernest F.
} Fullam, Inc. in Schenectady, NY. The cost is less than $100. This is
} in all probability the "aerosol" method you mentioned in your posting.
}
} 3] You have to worry about the glass transition temperature. If above
} room temperature, then you need not further worry. If below room
} temperature, then the particles at room temperature will be soft and
} you have to worry about the need to "harden" them up. Acrylic
} particles can be "hardened" for example by UV using quartz irradiation
} tubes.
}
} 4] While particles in this range could in theory be done by SEM,
} especially cryo SEM, you will find that Pt/C replication TEM will give
} much more acceptable results. Use a shadowing angle of 45 degrees.=20
} You can measure the "shadow" and also the apparent diameter, and then
} compare the measurements as a insight into any "collapse" of the
} particles, being sort of a built in validation that the particles are
} indeed hardened into "hard" rigid marbles.
}
} You do not need anything fancy in the way of a vacuum evaporator, any
} system giving a vacuum down in the "mid to low 10 to the minus five"
} range will give acceptable results.=20
}
} 5] If graft polymer latex, then if you want to see the morphological
} details of the graft vs. substrate polymer phase, you will have to use
} a still different technique to bring out such structures. But again,
} the method actually used will be determined by the specific polymers
} present and the kind of reactivity they exhibit.
}
} Chuck
}
} =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=
=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=
=3D=3D=3D=3D
} Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
} President 1-(800)-2424-SPI
} SPI SUPPLIES FAX: 1-(610)-436-5755
} PO BOX 656 e-mail:
} GVKM07A-at-prodigy.com
} West Chester, PA 19381-0656 USA Customer Service:=20
} SpiSupp-at-aol.com
} =20
} ######################################################## =20
} WWW: http://mail.cccbi.chester.pa.us/spi/spihome.html
=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=
=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=
=3D=3D=3D=3D=3D=3D
As Dr Charles A Garber wrote there are special problems to image polymers
in TEM. If you are not a polymer scientist, the negative staining method is
the most safe one. It will give a proper result even if you have a film
forming (low glass transition) or beam sensitive polymer. When you have
learnt to dilute the latexes and add appropriate amount of Uranyl Acetate
or PTA, it is also very easy to use.

Helen Hassander
Polymer group, Lund University

=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=
=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=
=3D=3D=3D=3D=3D=3D=3D
There is an article in the latest issue of THe Microscope that might be of=
=20
interest: "Cryo-Ultramicrotomy of Individual Latex Particles for Examination=
=20
of Internal Morphology", Marcelli, Angela. The Microscope=
43(3):117-120(1995)

Peter D. Barnett
Forensic Science Associates
e-mail: pbarnett-at-crl.com
Owen P. Mills
Michigan Technological University
Metallurgical & Materials Engineering
Rm 512 MME Building
Houghton, MI 49931
906-487-2002
906-487-2934 FAX
opmills-at-mtu.edu





From: l_thomas-at-ccmail.pnl.gov
Date: Thu, 30 Nov 1995 02:14 -0800 (PST)
Subject: Re[2]: SEM/EMPA: Anomalous BSE contrast...why??

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It might be a good idea to directly look at the BSE signal levels coming from
different parts of the sample rather than the differences of those signals.
Just to be sure that the 'image contrast reversal' isn't a product of the
electronics.

Larry Thomas
Battelle, PNL




From: Harold W Boone :      hboone-at-u.Arizona.EDU
Date: Thu, 30 Nov 1995 15:23:05 -0700 (MST)
Subject: Request for assistance

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Message-Id: {m0tLHwE-0002dTC-at-dewey.mindlink.net}

I have been contacted by an R&D group for a Pharmaceutical manufacturing
firm. They are interested in the integrity of the seal on gelatin
capsules. They haven't decided exactly in which direction to take the
project, but there are several possibilities:

1. Frozen sections, 1-5 microns thick, across the seal, for light
microscopy/F.T.I.R., etc.

2. Cryoultramicrotomy for EM of the seal

3. cryoultramicrotomy of seal area followed by SEM of the sectioned surface.

My schedule won't permit me to do the work. Is there anyone (preferably
in the southeastern US) who would like to work on this?

The R&D group is commited to doing the project, and they have a budget
for the work.

If you are interested, please contact me at rmcbtli-at-aol.comb (not the
address above) for additional information.

Bob Chiovetti
RMC




From: wangl000-at-HG.ULETH.CA
Date: Thu, 30 Nov 1995 20:30:44 MST
Subject: Reply of subscription

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Thank you for your admission of usename wangl000-at-uletg.ca
a
h







.ca

















From: Kukin V.N. :      lemi-at-mx.iki.rssi.ru
Date: Fri, 1 Dec 1995 12:23:19 +0300 (MSK)
Subject: E-mail address need

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For changing of scientific information we are needing E-mail addresses
following persons:
Prof. J.-P. Chavalier, C.E.C.M.-C.N.R.S., France;
Dr. A.G. Norman, Oxford University, UK;
Dr. J.L. Hutchison, Oxford University, UK;
Dr. W.M. Stobbs, Cambridge University, UK;
Dr. C.S. Baxter, Cambridge University, UK;
Dr. R.F. Broom, IBM Research Division, Zurich Research Laboratory, Sw;
Prof. S.Mahajan, Carnegie Mellon University, US;
Dr. I.T. Ferguson, Imperial College, London, UK;
Dr. S.J. Pennicook, Oak Ridge National Laboratory, US;
Dr. O. Ueda, Fujitsu Laboratories, Jupan.

Please reply on Microscopy list or directly on our E-mail addres:
lemi-at-mx.iki.rssi.ru
We will be grateful for any information.
Sincerely Yours Prof.S.Maksimov
CFPM, Electron microscopy laboratory,
Moscow Institute of Electronic Technology, Russuia.







From: kelloes-at-emlab.cb.uga.edu
Date: Fri, 1 Dec 1995 13:37:52 +0000
Subject: Durst enlarger parts

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Hello on the net:

I am desperately trying to find someone who has a Durst Laborator
S-45 EM enlarger that they no longer need. We are particular
interested in finding the NEGA-138 carriers with glasses. Also the
upper portion containing bellows and focusing devices. Any help would
be very much appreciated. We have two enlargers and need to keep
them in operational order. Thanks Cathy Kelloes




From: kelloes-at-emlab.cb.uga.edu
Date: Fri, 1 Dec 1995 13:37:52 +0000
Subject: Durst enlarger parts

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From: DONALD P. ROBERTSON :      dprobertson-at-TRENTU.CA
Date: Fri, 01 Dec 1995 13:44:54 -0400 (EDT)
Subject: Unsubscribe

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Unsubscribe Donald Robertson {dprobertson-at-trentu.ca}






From: dago-at-atlas.odyssee.net (Dagoberto Rodriguez)
Date: Fri, 1 Dec 1995 13:20:08 -0500
Subject: Sample prep - polymer nanoparticles

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We used to prepare nanoparticles for TEM by simply touching
a coated (carbon, formvar or collodium) grid directly on the
surface of the powdered sample.
We studied with this method the particle size and shape
distribution of several inorganic pigments, zeolites, polymers,
powdered bioinsecticides, etc.
It is good to measure particle dimensions, but particle
distribution is affected by preferential adsorption of particles
(For example in the range of sizes 50-250 nm for iron
pigments).
Dagoberto Rodriguez
Montreal, Qc, Canada





From: William Tivol :      tivol-at-wadsworth.org
Date: Fri, 1 Dec 1995 17:17:05 -0500 (EST)
Subject: Re: Al reading on EDXA

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} i frequently have Al detected when doing EDXA on plain carbon
} steel samples, even though i mount the samples using a carbon-
} based adhesive. However, i do not detect it all the time. any
} suggestions as to why this is occurring?
}
Dear Tania,
Is there any Al in your 'scope (liners, etc.)? Do you see Al when
you look at the background counts from a position right next to your sample?
Do you suspect there may be Br in the specimen (overlap with Al-k, but with
two peaks at } 10 keV)? Good luck.
Yours,
Bill Tivol




From: Christine A Brantner :      cab-at-csd.uwm.edu
Date: Fri, 1 Dec 1995 15:49:39 -0600 (CST)
Subject: Help!! holes in cells after immunostain

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Hello

I am a graduate student at the Univeraity of Wisconsin-Milwaukee and I have
a problem with a technique.

I embed bacteria in LR White. I did ultrastructural work and stained with
UA and Pb and had no major problem with holes in the resin. The next
project is to immunostain a protein in the membrane. I can use the same
blocks as before and after immunostaining, there are multiple holes in most
cells. I put the sections on nickel grids. NaCl for 1 min. New BSA 3%
filtered for 1 min. Primary antibody for 1 hour. BSA washes. Secondary
antibody IgG for 1 hour. BSA washes. NaCl wash. Water washes. The I
stain with UA for 1 hour as per usual.

Help.....I'd like to get rid of the holes. I think that it is happening
during this procedure. The new BSA has not eliminated them as was suggested
that it might.

Thank You

Chris Brantner

cab-at-csd.uwm.edu
University of Wisconsin-Milwaukee






From: Alan R. Burns :      aburns-at-bcm.tmc.edu
Date: Sat, 2 Dec 1995 19:28:06 -0600 (CST)
Subject: RE: TEM of Sorghum leaves

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---------- Forwarded message ----------

Phill:

Many years ago I used an ultra-low viscosity medium for rapid embedding
of plant cells. This resin prevented plasmolysis and the tissue
did not separate from the plastic. We published our findings and
the recipe for this plastic (available from Polysciences) in the
following article:

Oliveira et al., 1983. J.Microscopy 132:195-202.

Give it a try, it might just do the trick.






From: MelanieOwl-at-aol.com
Date: Sat, 2 Dec 1995 14:09:25 -0500
Subject: Non-stoichiometric iron oxide results with EDS

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Message-Id: {n1394184190.65950-at-QuickMail.Yale.edu}

We often do analyses of ground up deposits which contain iron oxides.
Sometimes, but not always, the EDS results will have an unreasonably large
amount of iron, with not enough oxygen, even though we know from XRD that the
material is iron oxide, not metallic iron. I don't know if it only occurs
with a particular iron oxide or not. Has anyone experienced this or have
some insight as to why this occurs?

Thanks in advance,
Melanie Behrens





From: zaluzec-at-Microscopy.Com (Nestor J. Zaluzec)
Date: Sun, 3 Dec 1995 22:08:41 -0600
Subject: Non-stoichiometric iron oxide results with EDS

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} X-UIDL: bc714d162b18bc45931c0b85533415ac
} Received: (from daemon-at-localhost) by Sparc5.Microscopy.Com (8.6.11/8.6.11) id
} NAA17979 for dist-Microscopy; Sat, 2 Dec 1995 13:10:52 -0600
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} Microscopy-at-microscopy.com; Sat, 2 Dec 1995 14:09:25 -0500
} From: MelanieOwl-at-aol.com
} Date: Sat, 2 Dec 1995 14:09:25 -0500
} Message-ID: {951202140924_123023520-at-mail06.mail.aol.com}
} To: Microscopy-at-microscopy.com
} Subject: Non-stoichiometric iron oxide results with EDS
} Content-Type: text
}
} We often do analyses of ground up deposits which contain iron oxides.
} Sometimes, but not always, the EDS results will have an unreasonably large
} amount of iron, with not enough oxygen, even though we know from XRD that the
} material is iron oxide, not metallic iron. I don't know if it only occurs
} with a particular iron oxide or not. Has anyone experienced this or have
} some insight as to why this occurs?
}
} Thanks in advance,
} Melanie Behrens
}
}






From: zaluzec-at-Microscopy.Com (Nestor J. Zaluzec)
Date: Sun, 3 Dec 1995 22:08:48 -0600
Subject: dusrt part needed also

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} X-UIDL: ae048b1c15b3a04492ad7391aa68499e
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} {microscopy-at-msa.microscopy.com} ; Sat, 2 Dec 1995 14:04:17 -0600
} Subject: dusrt part needed also
} To: microscopy-at-Sparc5.Microscopy.Com
} From: "Robert Schmitz, Biology" {rschmitz-at-macsrv1.uwsp.edu}
} Date: Sat, 2 Dec 95 14:08:42 +2000
} Message-ID: {951202.140842.13419-at-macsrv1.uwsp.edu}
} Content-Type: text
}
}
} } Subject: Durst enlarger parts
} }
} } Hello on the net:
} }
} } I am desperately trying to find someone who has a Durst Laborator
} } S-45 EM enlarger that they no longer need. We are particular
} } interested in finding the NEGA-138 carriers with glasses. Also the
} } upper portion containing bellows and focusing devices. Any help would
} } be very much appreciated. We have two enlargers and need to keep
} } them in operational order. Thanks Cathy Kelloes
}
} Cathy isn't the only only individual looking for Durst S-45 parts. I am
} looking
} for NEGA-138 carrier for 4x5 negatives and a Carlwen negative carrier (in
} particular, the negative carrier for 35mm film). I also would like to find an
} extra set of Latico 240 and 200 lens, as on of my colleagues claims ours has
} a
} hot spot that shows up in enlargements 3x or greater. Please reply to Cathy
} first but then if you can help me please contact me. Thanks!!
}
} rschmitz-at-macsrv1.uwsp.edu
} Bob Schmitz, Biology,
} Univ. WI Stevens Pt.
} Stevens Point, Wisconsin 54481
} ph 715-346-2420
}
}






From: zaluzec-at-Microscopy.Com (Nestor J. Zaluzec)
Date: Sun, 3 Dec 1995 22:08:54 -0600
Subject: RE: TEM of Sorghum leaves (fwd)

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} Sat, 2 Dec 1995 19:40:16 -0600
} Date: Sat, 2 Dec 1995 19:40:15 -0600 (CST)
} From: "Alan R. Burns" {aburns-at-bcm.tmc.edu}
} X-Sender: aburns-at-watson
} To: Microscopy-at-Sparc5.Microscopy.Com
} Subject: RE: TEM of Sorghum leaves (fwd)
} Message-ID: {Pine.SOL.3.91.951202193827.4291C-100000-at-watson}
} MIME-Version: 1.0
} Content-Type: TEXT/PLAIN; charset=US-ASCII
}
}
}
} ---------- Forwarded message ----------
} Date: Sat, 2 Dec 1995 19:28:06 -0600 (CST)
} From: Alan R. Burns {aburns-at-bcm.tmc.edu}
} To: Phill {aarwharn-at-reading.ac.uk}
} Subject: RE: TEM of Sorghum leaves
}
} Phill:
}
} Many years ago I used an ultra-low viscosity medium for rapid embedding
} of plant cells. This resin prevented plasmolysis and the tissue
} did not separate from the plastic. We published our findings and
} the recipe for this plastic (available from Polysciences) in the
} following article:
}
} Oliveira et al., 1983. J.Microscopy 132:195-202.
}
} Give it a try, it might just do the trick.
}
}
}






From: zaluzec-at-Microscopy.Com (Nestor J. Zaluzec)
Date: Sun, 3 Dec 1995 22:09:00 -0600
Subject: Re: TEM of Sorghum leaves

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} Date: 2 Dec 1995 21:04:25 -0500
} From: "Paul Webster" {Paul.Webster-at-quickmail.yale.edu}
} Subject: Re: TEM of Sorghum leaves
} To: "Microscopy BBS" {Microscopy-at-Sparc5.Microscopy.Com}
} X-Mailer: Mail*Link SMTP-QM 3.0.2
} Content-Type: text
}
} Subject: Time: 8:46 PM
} OFFICE MEMO Re} TEM of Sorghum leaves Date: 12/2/95
}
} In message {Pine.SOL.3.91.951129162920.2689F-100000-at-suma3.reading.ac.uk} Phill
} writes:
} }
} } Hello,
} } I have recently started doing TEM on the infection of sorghum leaves by
} } the fungus Colletotrichum graminicola. However, I have not had very much
} } success as of yet because I have had a lot of trouble fixing and
} } sectioning the leaf tissue. My main problems at the moment are poor
} } fixation and the resin splitting away from the leaf along the cuticle
} } when I am sectioning.
} Phil,
}
}
} The aldehydes normally used for EM fixation have been selected because they
} work
} well for mammalian tissue. They fix well because the material is full of
} primary amines which the aldehydes can crosslink. Plant tissue is very
} different and is therefore not easy to fix with these chemicals. Some
} suggestions which I have heard to solve the problem of using these chemicals on
} plants include adding extra primary amines to the material just prior to the
} fixation (this is the theoretical basis of Nakane fix, which has lysine added
} to
} the fixative solution, and to the fixative described by Luther & Block).
}
} Another good idea is to cool the plants down by leaving then in a fridge or
} cold
} room for a few hours and then fixing them in cold fixative This method I
} collected from a plant biologist, so I know it works. The logic behind the
} method can be easily demonstrated by trying to fix 2% gelatin at room temp. It
} will not fix because it is a liquid, but if it is cooled, the gel formed can be
} crosslinked by aldehyde. The fixed gelatin can be easily cut up and embedded,
} so should the leaves.
}
} Another alternative is to rapidly freeze the material by high pressure
} freezing,
} Muller et al (J. Microsc.) did this with apple leaves and achieved remarkable
} morphology in the fully hydrated sections they cut afterwards. Although it is
} technically demanding to freeze the material, the leaves need not be
} cryosectioned, but could instead be embedded in resin by freeze substitution -
} a
} process that only takes a day or two at most using simple materials.
}
} Whatever method you choose, we wish you good luck.
}
} Paul Webster
} Center for Cell Imaging
} Yale School of Medicine
} http://info.med.yale.edu/cellimg
}
}






From: zaluzec-at-Microscopy.Com (Nestor J. Zaluzec)
Date: Sun, 3 Dec 1995 22:09:06 -0600
Subject: TEM of Sorghum Leaves

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} Received: from apollo.roundlake.baxter.com (apollo.roundlake.baxter.com
} [159.198.1.9]) by Sparc5.Microscopy.Com (8.6.11/8.6.11) with SMTP id XAA00628
} for {Microscopy-at-MSA.Microscopy.Com} ; Sat, 2 Dec 1995 23:12:17 -0600
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} (v8.1.1-dmr001); Sat, 2 Dec 95 23:02:25 CST
} Received: by msmail_smtp.roundlake.baxter.com with Microsoft Mail id
} {30C14B8D-at-msmail_smtp.roundlake.baxter.com} ; Sat,
} 02 Dec 95 23:02:37 PST
} From: "Neuberger, Damian" {neuberd-at-engrnd.roundlake.baxter.com}
} To: "'Microscopy '" {Microscopy-at-Sparc5.Microscopy.Com}
} Subject: TEM of Sorghum Leaves
} Date: Sat, 02 Dec 95 23:10:00 PST
} Message-ID: {30C14B8D-at-msmail_smtp.roundlake.baxter.com}
} Encoding: 30 TEXT
} X-Mailer: Microsoft Mail V3.0
} Content-Type: text
}
}
} I don't have Phil's original message or address but would like to add a few
} comments to the already good ones submited.
}
} You should not be having a problem fixing Sorghum using typical plant
} fixation protocols, but without the specific details I can't comment
} further. However, a protocol for fixing Pinus resinosa seedlings, which
} were very difficult to fix and embed properly, utilized a 6% v/v
} glutaraldehyde and 6% paraformaldehyde v/v in 0.08 M cacodylate buffer at pH
} 7.2 for 2 hr. Following further dissection (I was looking at the phloem
} sieve elements), the tissue was additionally fixed for 10 hr at RT with a
} change of fresh fixative every hour. During this fixation step, the vials
} were on a rotator for continuous mixing. The tissue was washed in buffer
} and postfixed in cacodylate-buffered 2% OsO4 overnight in a refrigerator
} The reason for the changes is the presence of phenolic compounds that were
} reacting with the aldehydes (according to a biochemist). As for the poor
} infiltration of embedding medium, I used Spurr's low viscosity resin and
} diamond knives.
}
} If want to discuss this further and/or get references, we can do that
} off-line. Hope this helps.
}
} Damian Neuberger, Ph.D.
} Research Scientist
} Baxter Healthcare, Corp. MS WG3-2S
} P.O. Box 490
} Round Lake, IL 60073
} Tel: 708.270.5888
} Fax: 708.270.5897
} EMail: neuberd-at-baxter.com
}
}






From: zaluzec-at-Microscopy.Com (Nestor J. Zaluzec)
Date: Sun, 3 Dec 1995 22:09:11 -0600
Subject: Kevex & Tracor Northern

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} Sparc5.Microscopy.Com (8.6.11/8.6.11) with ESMTP id IAA00924 for
} {listserver-at-sparc5.microscopy.com} ; Sun, 3 Dec 1995 08:09:35 -0600
} Received: (from xianying-at-localhost) by strauss.udel.edu (8.6.12/8.6.12) id
} JAA16551; Sun, 3 Dec 1995 09:08:34 -0500
} Date: Sun, 3 Dec 1995 09:08:34 -0500 (EST)
} From: Xianying Burany {xianying-at-UDel.Edu}
} To: listserver-at-sparc5.microscopy.com
} Subject: Kevex & Tracor Northern
} Message-ID: {Pine.SOL.3.91.951203085038.14180B-100000-at-strauss.udel.edu}
} MIME-Version: 1.0
} Content-Type: TEXT/PLAIN; charset=US-ASCII
}
} I am looking for the email addresses of Kevex and Tracor Northern. I need
} help for our two EDSs. Please reply directly to me at
}
} xianying-at-udel.edu
}
} I greatly appreciate your help.
}
} Sandy Burany, PhD
} Dept. of Physics and Astronomy
} University of Delaware
} Newark, DE 19716
} (302) 831-3515
} Fax: (302) 831-1637
}
}






From: zaluzec-at-Microscopy.Com (Nestor J. Zaluzec)
Date: Sun, 3 Dec 1995 22:09:18 -0600
Subject: Al signal in EDS

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} {Microscopy-at-sparc5.microscopy.com} ; Sun, 3 Dec 1995 13:00:50 -0600
} Date: Sat, 02 Dec 1995 13:12:40 -0500 (CDT)
} Date-warning: Date header was inserted by uthscsa.edu
} From: "Nancy K. R. Smith" {SMITHN-at-UTHSCSA.EDU}
} Subject: Al signal in EDS
} X-Sender: SMITHN-at-arweN.UTHSCSA.EDU
} To: microscopy-at-aaem.amc.anl.gov
} Message-id: {01HYC02TNILU00AFQC-at-uthscsa.edu}
} X-Envelope-to: microscopy-at-aaem.amc.anl.gov
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}
} } Date: Fri, 01 Dec 1995 19:19:05
} } To: jeff_jones-at-mindlink.bc.ca
} } From: "Nancy K. R. Smith" {SMITHN-at-UTHSCSA.EDU}
} } Subject: Al signal in EDS
} }
} }
} } Tania Jones, c/o Jeff Jones:
} } The extraneous Al signal comes from the x-ray detector housing. X-rays
} and backscattered electrons strike the housing and generate Al x-rays. Some
} of those originating from just in front of the Be window are able to have
} line-of-sight entry into the window and be detected.
} }
} ..............................................................................
} .Post note: My email addresses are as follows:
} work nkrsmith-at-juno.com
} home smithn-at-uthscsa.edu
} BEST STRATEGY: address mail to smithn-at-uthscsa.edu and cc it to
} nkrsmith-at-juno.com. (Ignore the fact that the work & home addresses seem
} backwards.)
} ..............................................................................
} .Nancy K.Rodman Smith, Ph.D.
} .Dept. Cellular & Structural Biology
} .University of Texas Health Science Center at San Antonio
} .7703 Floyd Curl Drive
} .San Antonio TX 78284-7762
} .210-567-3861 Fax:210-567-3803
} .Email:SMITHN-at-UTHSCSA.EDU; NKRSMITH-at-JUNO.COM; NKRS-at-JUNO.COM
} ..............................................................................
} "God put me on earth to accomplish a certain number of things. Right now I
} am so far behind that I will never die." (Calvin, from Calvin & Hobbes
} cartoon)
} ****************************************************************************
} *****
}
}






From: Nancy K. R. Smith :      SMITHN-at-uthscsa.edu
Date: Sat, 02 Dec 1995 13:12:40 -0500 (CDT)
Subject: Al signal in EDS

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} Date: Fri, 01 Dec 1995 19:19:05
} To: jeff_jones-at-mindlink.bc.ca
} From: "Nancy K. R. Smith" {SMITHN-at-UTHSCSA.EDU}
} Subject: Al signal in EDS
}
}
} Tania Jones, c/o Jeff Jones:
} The extraneous Al signal comes from the x-ray detector housing. X-rays
and backscattered electrons strike the housing and generate Al x-rays. Some
of those originating from just in front of the Be window are able to have
line-of-sight entry into the window and be detected.
}
..............................................................................
.Post note: My email addresses are as follows:
work nkrsmith-at-juno.com
home smithn-at-uthscsa.edu
BEST STRATEGY: address mail to smithn-at-uthscsa.edu and cc it to
nkrsmith-at-juno.com. (Ignore the fact that the work & home addresses seem
backwards.)
..............................................................................
.Nancy K.Rodman Smith, Ph.D.
.Dept. Cellular & Structural Biology
.University of Texas Health Science Center at San Antonio
.7703 Floyd Curl Drive
.San Antonio TX 78284-7762
.210-567-3861 Fax:210-567-3803
.Email:SMITHN-at-UTHSCSA.EDU; NKRSMITH-at-JUNO.COM; NKRS-at-JUNO.COM
..............................................................................
"God put me on earth to accomplish a certain number of things. Right now I
am so far behind that I will never die." (Calvin, from Calvin & Hobbes cartoon)
****************************************************************************
*****





From: zaluzec-at-Microscopy.Com (Nestor J. Zaluzec)
Date: Sun, 3 Dec 1995 22:14:46 -0600
Subject: Re: Al reading on EDXA

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When looking into source of the extraneous Al signal
you should also investigate the composition of your
collimator to your EDS detector. You may have electrons
hitting the collimator creating x-rays which are then
detected by the SiLi system. This should be checked in
addition to the other components within the EM Chamber.

BTW, I presumed that you have a collimator, if not that
could also be part of the problem.

Also if you have a retractable EDS detector
is the Al signal a function of the detector position?

Your Friendly Neighborhood SysOp....

Nestor






From: Dr P. Echlin :      pe13-at-cus.cam.ac.uk
Date: Mon, 4 Dec 1995 08:36:21 +0000 (GMT)
Subject: Re: Help!! holes in cells after immunostain

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Christine Branter:

We regularly use LR White for immunocytochemistry of plant and microbial
material. Our schedule is as follows. Fix briefll ( the smaller the
sample the shorter the fixation- but typically 1H) Fixative is
2%paraformaldehyde + 0.5%glutaraldehyde in 50mMPIPES ph &.2-- you may
have to adjust this to your specimen) Dehydrate Ihour in each of 30, 50
70 and 80% EtOH at +4;-15;;-25 and again -25oC. Leave for 1H in 50:50 80%
EtoH;LR White. Leave overnight in the fresh 50:50 mixture, one hour in
fresh 50:50 mixture. Embed in fresh resin (Do all resin changes in an
inert atmosphere) Polymerize at -25oC for 24-48 hours. then place
capsules in a window to get what passes for sunlight in a Cambridge
winter.
Immuno- schedule is 3 x 5min in a blocking buffer; 4-16 hours in primary
antibody, wash x3; 90mins in a biotinylates secondary Ab; washx3; 90 mins
in streptavidin label (FITC,Texas Red colloidal gold etc.; wash x3 Ready
to go.
If you want detailds of various solutions e-mAil me direct

With Seasons Greetings

Patrick Echlin
Director
Multi-Imaging Centre
Schhol of Biological Sciences
University of Cambridge
United Kingdom

On Fri, 1 Dec
1995, Christine A Brantner wrote:

} Hello
}
} I am a graduate student at the Univeraity of Wisconsin-Milwaukee and I have
} a problem with a technique.
}
} I embed bacteria in LR White. I did ultrastructural work and stained with
} UA and Pb and had no major problem with holes in the resin. The next
} project is to immunostain a protein in the membrane. I can use the same
} blocks as before and after immunostaining, there are multiple holes in most
} cells. I put the sections on nickel grids. NaCl for 1 min. New BSA 3%
} filtered for 1 min. Primary antibody for 1 hour. BSA washes. Secondary
} antibody IgG for 1 hour. BSA washes. NaCl wash. Water washes. The I
} stain with UA for 1 hour as per usual.
}
} Help.....I'd like to get rid of the holes. I think that it is happening
} during this procedure. The new BSA has not eliminated them as was suggested
} that it might.
}
} Thank You
}
} Chris Brantner
}
} cab-at-csd.uwm.edu
} University of Wisconsin-Milwaukee
}




From: Tom Bryner :      brynert-at-mcmaster.ca
Date: Mon, 4 Dec 1995 11:54:47 -0500
Subject: Image Analysis

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We have a grad student looking for an Image Analysis program to run
on a PC. He is looking at polymer images on an optical microscope and wants
to measure particle size, distribution and fibre length.

Tom Bryner







From: VETO-at-BCRSSU.AGR.CA
Date: 04 Dec 1995 12:59:56 -0400 (EDT)
Subject: Re: Al signal on EDXA

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You may "paint" your collimator inside and outside with pure
colloidal graphite (carbon paint) to minimize extraneous Al signal.

Laszlo J. Veto




From: VETO-at-BCRSSU.AGR.CA
Date: 04 Dec 1995 13:16:51 -0400 (EDT)
Subject: Re: TEM of Sorghum leaves

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Phill:

In addition of the other good ideas, try to use 0.18 M PIPES buffer at
pH 6.8 vith 0.5 to 1.0% Caffeine, in BOTH fixative (ald. & osmium)
Prepare solutions immediately before use. Extra long fixation times!
All the best,

Laszlo J. Veto




From: oshel-at-ux1.cso.uiuc.edu (Philip Oshel)
Date: Mon, 4 Dec 1995 18:51:31 -0500
Subject: Re: Al signal on EDXA

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Message-Id: {v02120d00ace938cf11d4-at-[128.174.23.57]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

} You may "paint" your collimator inside and outside with pure
} colloidal graphite (carbon paint) to minimize extraneous Al signal.
}
} Laszlo J. Veto

Make sure it's pure graphite! I've found carbon paints sold for
mounting specimens for EDX that are contaminated with phosphorus .
Phil Oshel

&&& Illigitimi non carborundum &&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&
Philip Oshel
Center for Electron Microscopy
University of Illinois
(217)244-3145
oshel-at-ux1.cso.uiuc.edu






From: rutledge phil :      prutle1-at-gl.umbc.edu
Date: Mon, 4 Dec 1995 11:04:49 -0500 (EST)
Subject: Re: dusrt part needed also

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Durst users:

I was able to get Durst parts from the following companies. Hopefully
they are still in business.

Nutek (312) 564-3070
3206 Doolittle
Northbrook, Illinois 60062

Colenta (201) 265-5670
348 Evelyn St.
Paramus, NJ 07652

for negative carriers:

Carlwen Industries Inc. (301) 469-6671
P.O. Box 1748
Rockville, MD 20850

35mm film: 37TB frame to fit L-35 base, if you don't have the base,
you have to order the L-35 base with opening for 35mm film. The frame has
focusing target and both are black anodized.
At Carlwen I talked to a Mr. Locken.

As I said, I do not know if either of the 3 companies are still in
business or if they have moved. My P.O.s go back almost 10 years. It's
certainly worth a try. Hope this helps.

Peace,

Phil Rutledge, Director
Center for Electron Microscopy
UMBC
Dept. of Biology
Catonsvill, MD 21228




From: James R. Stets :      stetsjr-at-ttown.apci.com
Date: Tue, 5 Dec 1995 08:48:41 -0500 (EST)
Subject: EDS: Al in Iron Oxide Discussion

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A few thoughts on Melanie Behrens' posting regarding the aluminum peak in
the EDS spectrum of the iron oxide:

First, I would agree that the problem is in the specimen and not an
artifact from the microscope/detector since your colleague can obtain the
same result using a different instrument. You might also try analyzing a
sample that you are absolutely certain contains no aluminum using the
same instrument settings (accelerating voltage, count rate, etc.) and see
if the aluminum peak appears.

You also commented, "The sample was ground up but not prepared in epoxy
and polished or coated, since we are not looking for a strictly
quantitative result and want something of a bulk analysis as fast as
possible." Are you running this truly quantitative (comparing it against
a known standard) or are you using a "standardless miracle" routine, as
the DTSA folks call it? This could cause you a lot of headaches.

Since you ground up the sample, and are after a bulk analysis, wouldn't
it make more sense to analyze it via XRF?

Jim Stets
Air Products and Chemicals, Inc.
Allentown, PA
stetsjr-at-apci.com






From: gwe-at-biotech.ufl.edu (Greg Erdos)
Date: Mon, 4 Dec 1995 11:15:44 GMT
Subject: EDS: Al in Iron Oxide Discussion

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confocal-at-ubvm.cc.buffalo.edu, cytonet-at-net.bio.net.edu,
dd-email-list-at-worms.cmsbio.nwu.edu, rogers_bill/furman-at-furman.edu,
odonoghuee%levicri-2-at-pncri-1.palm.cri.nz, clausnz-at-itsa.ucsf.edu,
roosg-at-ezinfo.vmsmail.ethz.ch, MLSB86A-at-PRODIGY.COM,
horst.mn-at-gain.Mercer.EDU, bergrh-at-msuvx2.memphis.edu,
jdonald-at-deakin.edu.au, lclemen-at-junix.ju.edu,
lynn-at-daisy.moffitt.usf.edu, roosgrp-at-botinst.unizh.ch,
wiersma-at-ralvm8.vnet.ibm.com, Microscopy-at-Sparc5.Microscopy.Com,
nread-at-srv0.bio.edinburgh.ac.uk, ophansti-at-pegasus.cc.ucf.edu,
ptks-at-festival.ed.ac.uk, ralphr-at-pop.ben2.ucla.edu,
howardrj-at-ESVAX.DNET.DUPONT.COM, rmbrown-at-ccwf.cc.utexas.edu,
ttoop-at-hestia.ccs.deakin.edu.au, tjraub-at-pwinet.upj.com,
roos-at-botinst.unizh.ch, gwe-at-biotech.ufl.edu, sdw-at-biotech.ufl.edu,
klv-at-biotech.ufl.edu, gwerdos-at-gnv.ifas.ufl.edu,
emcore-at-icbr.ifas.ufl.edu


Please note that the telephone area code for Gainesville Florida and
the University of Florida has been changed to:

(352)-
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Greg Erdos Phone: 352-392-1295
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ICBR Electron Microscopy Core Lab
218 Carr Hall Fax: 352-846-0251
University of Florida E-mail: gwe-at-biotech.ufl.edu
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From: Dwight Beebe :      beebed-at-ere.umontreal.ca
Date: Mon, 4 Dec 1995 13:43:07 -0500 (EST)
Subject: Re: TEM of Sorghum leaves

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Greetings:
Although I appreciate that fact that information from a plant
biologist can be considered as gospel truth, I would just like to point
out that the effects of cold treatment (chilling) can be considerable,
from both a metabolic and morphological point of view. I would suggest
that the first hour or so of the fixation should be carried out at room
temperature, followed by an equal length of time (or longer) at 4 C. The
initial fix at room temperature will act to preserve the tissue without
introducing any structural variation due to chilling. I have a protocol
that I can provide, that works well with dicot leaves. If interested
drop me a note and I'll send it off.
Damian makes reference to the use of both para- and
glutaraldehyde to fix difficult tissue. I also use both in standard
chemical fixations to enhance the likelyhood of rapid and adequate
fixation. I use a lower percentage of PA, but it will vary according to
the tissue type and the desired level of fixation.
Best of luck with the fixation.

Dwight Beebe
Institut de recherche en biologie vegetale beebed-at-ere.umontreal.ca
Dept. de sciences biologiques Voice:514-872-4563
Universite de Montreal FAX:514-872-8496
4101, rue Sherbrooke est
Montreal, PQ H1X 2B2 Canada





From: Ing. Jiri Spinka UETE :      spinka-at-uete.fee.vutbr.cz
Date: Tue, 5 Dec 1995 07:55:58 MET-1MEST
Subject: contrast improvment, fixation

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Dear microscopists,
I need some information about fixation and contrast improvment of
ovocyts and spermas for ESEM.
I have no experiences with such of type of specimens.
I ask you to give me some advices about preparing and technique.
Thank You.

===========================================================
Jiri Spinka
Faculty of Electrical Engineering and Computer Science
Department of Electrotechnology
Technical University of Brno EEEEEE TTTTTT
Antoninska 1, B R N O EE TT
Czech Republic EEEE TT
Tel. 42-5-753741, Fax. 42-5-41211135 EE TT
e-mail: spinka-at-uete.fee.vutbr.cz (Internet) EEEEEE(hi) TT
===========================================================




From: MelanieOwl-at-aol.com
Date: Mon, 4 Dec 1995 22:10:36 -0500
Subject: Iron oxides by EDS Clarifications

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I am sending this response to the listserver so that each of those who wanted
further details about the problem could get a more detailed description.

The detector is a Noran thin window light element detector with 134 eV
resolution, with a Voyager II workstation. The detector has not been
serviced for about a year, so cleanliness or ice buildup is possible, but the
problem does not occur with all iron oxides or mixtures of metals with light
elements, only with specific samples. A different instrument with a Kevex
detector at another of our company's locations has also experienced this
problem with the same samples, so I believe it is a sample problem, not a
detector problem. The background shape appeared to be normal.

The sample may be a mixture of oxides, but the stoichiometry is so far off
that even FeO would not account for it, and the XRD (if I remember correctly,
since it has been awhile since I last saw this phenomenom) did not report FeO
exclusively. I will check on this tomorrow at work. I will also check what
other elements were present, but I believe the EDS gave the result as over 90
wt% iron, with perhaps 5 wt% oxygen, if that. The sample was ground up but
not prepared in epoxy and polished or coated, since we are not looking for a
strictly quantitative result and want something of a bulk analysis as fast as
possible. I think it is natural iron oxide, since we did not synthesize it in
the lab - it was found in an unwanted location. It did not appear to be iron
metal either by XRD or BSE image.

Hope this answers some of your questions and encourages more discussion.
Thanks to all who have already replied to my posting.

Melanie Behrens
Senior Chemist
Texaco, Inc




From: Mark T. Biedrzycki :      mtb-at-bigdog.engr.arizona.edu
Date: Tue, 5 Dec 1995 11:00:01 -0700 (MST)
Subject: Re: Al reading on EDXA

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} i frequently have Al detected when doing EDXA on plain carbon
} steel samples, even though i mount the samples using a carbon-
} based adhesive. However, i do not detect it all the time. any
} suggestions as to why this is occurring?
}
} thanks,
}
} tania jones
Is the mounting stub made of Al and the sample 'relatively' thin?
Recall the "volume of interaction" is typically greater
than what you are viewing at 1000x...

Best regards,

Mark T. Biedrzycki
Computer Networking Laboratory Materials Science and
for Microscopy Education (CNLME) Engineering Department
at the University of Arizona, Tucson





From: VETO-at-BCRSSU.AGR.CA
Date: 05 Dec 1995 15:16:54 -0400 (EDT)
Subject: Re: buffer containing caffeine

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Jay,

"When phenolic-containing plant cells are fixed with glutaraldehyde
followed by OsO4, phenolics leach from the vacuoles into the cytoplasm
where they subsequently react with OsO4. The result is a dense, osmiophilic
cytoplasm, the details of which are obscured. This leaching can be prevented
by fixation with glutaraldehyde containing 0.1-1.0% caffeine and then a
rinse with a buffer containing caffeine."

Ref.: Hayat, M. A. (1986). "Basic Techniques for Transmission Electron
Microscopy" Academic Press. Inc., Orlando, Florida. ISBN 0-12-333925-1
Hope it helps, regards,

Laszlo J. Veto.




From: preynold-at-itsmail1.hamilton.edu (Patrick Reynolds)
Date: Tue, 5 Dec 1995 16:30:49 -0500
Subject: Histological tissue processors

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Hello all,
I would be grateful if anyone could point me in the direction of
companies that manufacture or deal in tissue processors, for histological
(paraffin embedding) use. I have turned up nothing through our sources
here; Fischer discontinued their line.
Thank you in advance,
Pat Reynolds
___________________________
Patrick D. Reynolds
Biology Department
Hamilton College
198 College Hill Rd.
Clinton, N.Y. 13323
Voice: (315) 859-4723
Fax: (315) 859-4807





From: WXCH07A-at-PRODIGY.COM (MR THOMAS A EBY)
Date: Tue, 05 Dec 1995 19:48:55 EST
Subject: Fracturing polymer fibers

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X-Nupop-Charset: English

Anyone out there have a good technique for obtaining fracture
surfaces of fine (10-20um dia) fibers that remain flexible even at
LN2 temps? Trying to get SEM images of fracture sections without
smearing associated with microtomy or other cutting.
Thanks





From: WXCH07A-at-PRODIGY.COM (MR THOMAS A EBY)
Date: Tue, 05 Dec 1995 19:47:07 EST
Subject: Fracturing polymer fibers

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Anyone out there have a good technique for obtaining fracture
surfaces of fine (10-20um dia) fibers that remain flexible even at
LN2 temps? Trying to get SEM images of fracture sections without
smearing associated with microtomy or other cutting.
Thanks





From: OptoMech-at-aol.com
Date: Tue, 5 Dec 1995 22:03:30 -0500
Subject: Re: Custom microscopy solution?

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We handle all types of repairs, modifications and custom design work for
microscopy. We also do a lot of Imaging and aquisitions from the microscope.
Please call us if you have any questions.


Opto-Mechanical Consultants
2102 Riding Crop Way
Baltimore, MD 21244
410-944-1721 phone/fax




From: MelanieOwl-at-aol.com
Date: Tue, 5 Dec 1995 20:25:34 -0500
Subject: Re: Iron oxides by EDS Clarifications

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Yes, I am using a standardless PROZA routine and yes, I realize this is not
the ideal way of performing quantitative analysis. However, I have gotten
acceptable although not perfect results with other samples containing iron
oxides. I think, however, that you may be right about the sample effects of
grain size and orientation possibly being the reason for the problem.
Thanks,
Melanie Behrens
Senior Chemist
Texaco, Inc

You wrote:
One thought to check for instrumental vs. sample effects in your
anomalous Fe-oxide is to look not only at the Fe/O ratio, but also the
Fe-K/Fe-L ratio (assuming that you are analyzing Fe by measuring the
K-alpha line). Because of my WDS background I don't have a good opinion
how well crushed samples work, but consider that with over a factor of 10
difference in energy, Fe and O information does not come from strictly
the same part of the sample. Grain size or even grain orientation might
make a big difference in the relative sizes of the Fe and O peaks. I
studied this effect once (although unfortunately I don't have the data at
hand any more) with Si, Ca, and Fe (much more similar to each other in
energy than Fe and O), and concluded that it was hardly possible to
distinguish Ca3Fe2Si3 and CaFeSi2 unless I was working with a surface of
known orientation!

A further question--when you say over 90% Fe, is that relative to a
standard, or are you using a "standardless" precedure that normalizes to
100% (another thing that I distrust with my WDS bias).

Best wishes,
Alfred
---
Alfred Kracher
akracher-at-iastate.edu
http://www.public.iastate.edu/~akracher/







From: Zhiyu Wang :      zhiyu-at-hawaii.edu
Date: Tue, 5 Dec 1995 21:22:22 -1000
Subject: Re: Image Analysis

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This is a NOT an ad.

Try Mocha Images which works on PC.


Wang



On Mon, 4 Dec 1995, Tom Bryner wrote:

} We have a grad student looking for an Image Analysis program to run
} on a PC. He is looking at polymer images on an optical microscope and wants
} to measure particle size, distribution and fibre length.
}
} Tom Bryner
}
}




From: Dr P. Echlin :      pe13-at-cus.cam.ac.uk
Date: Wed, 6 Dec 1995 08:52:12 +0000 (GMT)
Subject: An alternative transition fluid to replace halocarbons

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Has anyone got a suggestion for an alternative transition fluid to
replace the now banned and unavailable halocarbons which have been used
in critical point drying using either ethanol of acetone. I'm not sure I
want to use amyl acetate as its a bit toxic and remains in the seals of
the critical point dryer.
For Cambridge watchers, it's two degrees celcius and snowing

Patrick Echlin
Multi-Imaging Centre
School of Biological Sciences
University of Cambridge.




From: Dr P. Echlin :      pe13-at-cus.cam.ac.uk
Date: Wed, 6 Dec 1995 08:58:25 +0000 (GMT)
Subject: Re: TEM of Sorghum leaves

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Caffeine takes care of the poly=phrnols which abound in many plant
tissues.

Patrick EchlinOn Mon, 4 Dec 1995, Jay Jerome wrote:

}
} Lazlo-
}
} Why is caffeine added to the buffer?
}
}
} Jay Jerome
} **************************************************************
} * aka: W. Gray Jerome *
} * Dept. of Pathology *
} * Bowman Gray School of Medicine of Wake Forest University *
} * Medical Center Blvd *
} * Winston-Salem, NC 27157-1092 *
} * 910-716-4972 *
} * jjerome-at-bgsm.edu *
} **************************************************************
}
} On 4 Dec 1995 VETO-at-BCRSSU.AGR.CA wrote:
}
} } Phill:
} }
} } In addition of the other good ideas, try to use 0.18 M PIPES buffer at
} } pH 6.8 vith 0.5 to 1.0% Caffeine, in BOTH fixative (ald. & osmium)
} } Prepare solutions immediately before use. Extra long fixation times!
} } All the best,
} }
} } Laszlo J. Veto
} }
}




From: tphillips-at-biosci.mbp.missouri.edu (Tom Phillips)
Date: Wed, 6 Dec 1995 10:12:43 -0500
Subject: PPM to TIF converter?

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Message-Id: {199512061509.KAA14889-at-post-ofc02.srv.cis.pitt.edu}
To: "microscopy-at-aaem.amc.anl.gov" {microscopy-at-aaem.amc.anl.gov}

Does anyone know of a PPM to TIF converter (freeware, shareware or even
commercial)?

Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility
3 Tucker Hall
University of Missouri
Columbia, MO 65211
(314)-882-4712 (voice)
(314)-882-0123 (fax)






From: RonMervis-at-aol.com
Date: Wed, 6 Dec 1995 11:29:26 -0500
Subject: subscribe

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please add me to your list:
Ronald F. Mervims, Ph.D. (Ron Mervis-at-aol.com)
President
NeuroMetrix Rearch, Inc.
2109 West Fifth Ave., suite B
Columbus, Ohio 43212




From: llsutter-at-mtu.edu (Larry Sutter)
Date: Wed, 6 Dec 1995 11:35:31 -0500
Subject: Cement Microscopy Conference

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I am trying to find information on a cement microscopy conference
to be held in Houston (?) this February (?). Any information would be
appreciated. Thanks...






Larry Sutter
Michigan Technological University
Dept. of Civil and Environmental Engineering
1400 Townsend Dr.
Houghton, MI 49931

voice: 906-487-2423
FAX: 906-487-2943

e-mail: llsutter-at-mtu.edu
WWW: http://www.civil.mtu.edu/~llsutter/






From: GVKM07A-at-PRODIGY.COM (DR CHARLES A GARBER)
Date: Wed, 06 Dec 1995 10:41:40 EST
Subject: Request for advice

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-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --

We have in our laboratory a client who has a soft contact lens
(polyhydroxyethyl methacrylate) that has been exposed to a dye, of the
type that gives soft lenses different colors. The question is one of
determining to what degree the dye is chemically bonded to the polymer
vs. being entrapped in the polymer's porosity.

The less than 0.1 Wt. % of dye loading would seem to me to preclude any
possible use of XPS. While SIMS is a lot more sensitive, I would not
have thought one could get useful information of this type at these
levels.

Wondered if anyone out there has ever been confronted with this kind of
a problem and would offer any suggestions.

Thanks in advance.

Chuck

======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail:
GVKM07A-at-prodigy.com
West Chester, PA 19381-0656 USA Customer Service:
SpiSupp-at-aol.com

########################################################
WWW: http://mail.cccbi.chester.pa.us/spi/spihome.html
########################################################
======================================================








From: Stefan.Gunnarsson-at-devbiol.uu.se (Stefan Gunnarsson)
Date: Wed, 6 Dec 1995 18:06:13 +0100
Subject: Re: An alternative transition fluid to replace halocarbons

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X-Sender: zoogun-at-strix.udac.uu.se
Message-Id: {v01510101aceb7bb4a212-at-[130.238.80.10]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

} Has anyone got a suggestion for an alternative transition fluid to
} replace the now banned and unavailable halocarbons which have been used
} in critical point drying using either ethanol of acetone. I'm not sure I
} want to use amyl acetate as its a bit toxic and remains in the seals of
} the critical point dryer.
} For Cambridge watchers, it's two degrees celcius and snowing
}
} Patrick Echlin
} Multi-Imaging Centre
} School of Biological Sciences
} University of Cambridge.


We use acetone or in some cases ethanol straight through to the CO2 and for
most purposes it works just as well. We increase the times a bit and make
sure that the solvents are as waterfree as possible. If it is very critical
you will need a molecular sieve to dry the solvent and also a water trap
for the CO2.

We also tried dimethoxypropane some years ago. It works both for
dehydration (it forms acetone and methanol when mixed with water) and
subsequently as transition fluid. As I recall the result was about the same
as with the normal procedure (dehydration with ethanol and Freon as
transition fluid).

Hope this helps.

Uppsala is not any better, it's been down to -10 celcius the last couple of
days and generally unpleasant.

Stefan


..............................................................................

Stefan Gunnarsson
Microscopy Unit,Dept. of Animal Development and Genetics

Uppsala University
Norbyv. 18A, S-75236 UPPSALA, Sweden
e-mail Stefan.Gunnarsson-at-devbiol.uu.se








From: BARRY PYLE :      umbbp-at-msu.oscs.montana.edu
Date: Wed, 06 Dec 1995 14:51:55 MST
Subject: Unsubscribe

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From: dlb-at-u.Arizona.EDU (David Bentley)
Date: Wed, 6 Dec 1995 09:00:49 -0700
Subject: Re: An alternative transition fluid to replace halocarbons

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If you get direct posts, would you please forward them to the
listserver. I too, am interested in a substitute, and have not been very
happy with the quality of the CPD material since we can't realistically use
Freon TF. I am under the impression that even freshly opened alcohol,
within a few seconds absorbs water, but it may be even more complex than
that, such as miscibilities of alcohol and CO2.
We have had numerous suggestions such as drying the CO2 tank out
before refilling at the supplier, but I am afraid that our supplier is not
that cooperative. To go the the next better grade of CO2 the price per tank
goes from $15.00 to $130.00, and the specified composition with regard to
water improves only marginally (I believe from 0.2% water to less than 0.1%
water). A rather pricey experiment. We have a drier in the CO2 line and
condense CO2 gas into the chamber, rather than use a siphon tube but these
do little to improve the quality. Peldri II does work slightly better than
CPD but does seem somewhat material dependent, and is relatively more
expensive. Lastly, it should be mentioned that we do a large number of
samples in a Polaron Jumbo CPD, so we do go through a fair amount of reagents.
I have been reluctant to use anything thus far besides alcohol both
from the point of view of flammability and toxicity. During the winter
months, with low humidity, on release of the CO2 there is a lot of static
discharges. Knock on wood, no ignitions yet, but I am concerned.
Any responses that appear or that you pass along will be appreciated.
later dlb

David Bentley
Imaging Facility, Div. of Biotechnology, ARL
The University of Arizona
Tucson, Az 85721





From: em-at-mediacity.com (E. Monberg)
Date: Wed, 6 Dec 1995 14:29:47 -0800
Subject: HITACHI ACOUSTIC MICROSCOPE AT-5000 MANUAL

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Help !

Who knows the telephone number and address

for the Hitachi Office supporting Acoustic Microscopes ?

I need a manual for model AT-5000

Thanks Much !




Regards,




Ed Monberg, GM em-at-mediacity.com
LMDC (Laser Motion Development Company)
3101 Whipple Road Union City, CA 94587-1216
510-429-1060 voice
510-429-1065 fax


Inventory Listing:

Send empty e-mail to: Cat-at-LaserMotion.com






From: John Hunt :      hunt-at-msc.cornell.edu
Date: Tue, 5 Dec 1995 15:33:58 -0500 (EST)
Subject: Re: Solder Analysis

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Message-Id: {199512061630.KAA04229-at-Sparc5.Microscopy.Com}

Fellow Microscopists:

Several months ago I asked a question about solder analysis via EDS. I
promised to post a summary of the replies and had not done so. Here are the
replies I received. I enclosed the majority of the replies. You will have to
judge the techniques yourself, since we have not progressed any further in our
quest, due to time and/or budget constraints.

I apologize for not doing this sooner.

If you get any good results please let me know.

John Giles

Here's the original post followed by the replies:
------------------------------------------------------------------------------

Hello,

I am interested on the community input on performing in-situ analysis of solder
to determine composition. We have tried to do this in the past and have not
had acceptable results. We used both multiple areas and multiple points and
averaged the data.

I realize that this is probably an impossible task with EDS, but would like to
confirm my opinion and obtain input as to any other techniques (micro probe
XRF, etc.) which may provide a non-destructive method for in-situ solder
analysis.

Thanks,

John Giles
jegiles-at-space.honeywell.com
---------------------------------------------------------------------------


The beast raises its ugly head again.

Yes, you can get quant info this way. I have done it, but it is not very
accurate. If you have a reference sample of a known solder you can use
image processing to get a good idea of the solder composition you are
examining. This is purely subjective of course, which is why you need a
reference sample. You need to do a histogram analysis of the unkown and
reference samples under the same conditions. The method you use to get the
numbers to look correct on the reference is repeated on the unkown sample.
This will usually get you ballpark type numbers, but again it is purely
subjective. We can discuss this method further if you wish.

A far better way is to use an XRF thickness machine. There are standards
available for the common binary solders. This method is much faster. We
have gotten bars of trinary solders chemicaly analyzed and plan to set up
calibration files for these solders. So far no one has had the time to do
this.

What is your objective? Are you trying to verify the type of solder used or

are you trying to profile the change in composition under different
conditions?

Al
----------


Al
We have been getting a lot of conflicting data about analyzing solder on
pwb's.
Some people are claiming that they can get quant info by a combination of
image analysis and eds. Know anything about this?

Ron
-------------------------------------------------------------------

John,
It seems to work best when I can polish the sample and look at an area at
very low magnification. Low magnification being 100X in the SEM. For
instance, making a polished section to check the composition in the solder
pot for solderability testing. In general, the lower the magnification, the
better the overall results. Looking at points hasn't worked.

A fellow metallurgist at GE Cincinnati says that you have to take photographs
of many different areas, drop a ruler and get grain size and/or volume
fraction of the two phases, and then calculate the composition. A backscatter
detector would probably work the best to highlight the two phases. Old
fashioned image analysis. Some of the new EDS systems have these
capabilities-ours doesn't.

Good luck,
Sharon

-------------------------------------------------------------------

Dear John,

You are certainly correct with regard to homogeneity, but enough area samplings
should be somewhat representative. We have a Link LZ5 detector and a Dapple
System analyzer/software. Given consistent operating conditions, sample
condition, good reference standards, etc, you should be able to get good
results.

We have a good relationship with our supplier, Dapple, and have run quant
studies for them. Though I had extreme difficulty with Kevex in the past, I
believe those individuals have been replaced. Have you tried their tech
support?

I will give you Bill Stewart's (Dapple System) telephone number. If Kevex
cannot or has not helped you, then try calling him. He can tell you what
limitations you can expect from EDS in general and if your Kevex system is
insufficient for the task. If you do call Bill, tell him I suggested you
contact him. I have always found him to be very helpful.

Bill Stewart
Dapple Systems
408/733-3283

Please send me an update. Good Luck.

Alan Stone
ASTON Metallurgical Services
------------------------------------------------------------------------

John Giles,

I am forwarding you some information I rec'd from a faculty member here
in the Metallurgy department at Michigan Tech. Hope this is some help to
you.

Owen P. Mills
Dept. of Metallurgical & Materials Engineering
Michigan Technological University
Houghton, MI 49931
906-487-2002
opmills-at-mtu.edu

---------- Forwarded message ----------

Owen:

I haven't done any work, but have maintained a passing interest in
soldering as related to wetting during joint formation. Aside from the
standard SEM/EDS references, the following book (though not directly
addressing the problem) might have some secondary references related to
analysis of solders:

Solder Mechanics-A State of the Art Assessment
D. R. Frear, W. B. Jones, and K. R. Kinsman
TMS, Warrendale, PA

Authors of the various articles will also be good sources for information.
I know that several of them are from Sandia Albuquerque which has had a
large program on soldering for several years.

Cal
Calvin L. White
Department of Metallurgical and Materials Engineering
Michigan Technological University
Houghton, MI 49931
phone (906) 487-2036
fax (906) 487-2934
email cwhite-at-mtu.edu
---------------------------------------------------------------------

Hello John,
Here at Princeton we have a 6 spectrometer (5 WDS/EDS) EPMA system that we
use for a wide range of nondestructive analyses. ALthough I'm unfamiliar
with your samples I see no reason your problem couldn't be solved by
wavelength spectroscopy. If you are interested you could send me a
specimen and I could produce some results for you. Just let me know.
Ed Vicenzi



----------------------------------------------------------------------

I have had a lot of problems determining the composition of solder with EDS.
If life is good, and we process escape peaks, remove the background and
quantify, the results will be very close. It is impossible if it is high
temperature solder which may include 1-2% silver, whose peaks are severely
overlapped with lead and tin. Since we usually just want to know the melting
point of the solder, and the samples can be destroyed, we are now using
differential scanning calorimetry (DSC) to determine the solder melting
point. This can be correlated to the composition of standard tin-lead
solder. The sample size is relatively small and the equipment is
comparatively inexpensive. Most service labs have it also.

Not quite what you asked,
Sharon
-------------------------------------------------------------------------


John,
Having been frustrated by solder I thought I would pass on my "wisdom." The
problem with trying to analyze solder by any microbeam technique is the
nature of the material as it solidifies. I assume you are dealing with a
tin-lead system on the lead rich side of the eutectic composition. Because
solder solidifies through the same "pasty" range (liquidus-solidus region
above the eutectic temperature and below the liquidus temperature) it went
through on fusion the composition will vary throughout the mass beginning
where the first nucleation occurred and ending with the material that
solidified last. If you follow the solidification process on the Pb-Sn
equilibrium diagram you will get an idea of the potential problems brought
on by compositional variations. Also, when solder solidifies the size of
the individual grains will vary due to localized compositional variations,
and more importantly the rate of change of temperature during the
solidification process. From previous experience in attempting to do
semi-quantitative analysis of solders using an SEM/EDS approach we soon
discovered it is not practical to do it in situ. Our procedure was to carve
away a good size chunk of solder, place it between two steel plates then
flatten and thin it out with a healthy "calibrated" hammer blow. The
flattening reduced the compositional variation due to depth by spreading it
out and making it more accessible to excitation. Collecting a number of
spectra which covered most of the surface area which would be summed
together and used for "quantification" gave reasonable results when compared
with known materials handled in the same way. Trying to use a beam in spot
mode, whether in an SEM or EPMA, would not work because of the uncertainty
of which phase in the solder the beam is exciting. XRF holds a little
better hope, particularly if you can use the higher energy lines, i.e., Sn
-K and Pb-L for analysis and have an EDXRF system. However, what do you use
for standards? and how do you account for geometry affects? Wavelength
methods would be very difficult without precise geometry relationships.I
think you get the point that I don't believe in situ is the way to go!

I hope this input has been of some value to you, and good luck! I would be
interested in hearing how you make out with this search, could you post a
summary of responses.

Bob Craig
OSRAM SYLVANIA INC.
Lighting Research Center
Beverly, MA 01915
craig-at-hq.sylvania.com
---------------------------------------------------------------------------

John,
We're looking at approx 0.5 mm dots of solder mounted on aluminum stubs.
We haven't needed to check solder on circuit boards, but that ought to be
possible with appropriate grounding. The WDS has been used to look at
oxygen and some trace elements. We use ZAF quantitation with EDS to look
at ratios of lead and tin. We haven't done any absolute quantitation with
standards, but that ought to be possible too. Would you like to send a
specimen here and see what our spectra look like?
Mike
----------------
} Thanks for your reply. The specific application I am looking at is analyzing
} solder joints on a PC board. One of our production engineers would like to
use
} this as an inspection technique for solder composition as opposed to our
} typical analysis of bulk samples from the solder pot prior to soldering. I do
} not have a WDS on our system, but would be interested in the results you get
on
} a known solder standard.
}
} John

----------------------------------------------------------------------------

John,
At MCNC, we've been analyzing solder using a combination of EDS and WDS.
Those techniques are nearer to bulk composition analysis than the
surface-oriented techniques are. Maybe I didn't understand exactly what
you need to analyze. If you want to send more details, I could pass on
your questions to our group that works on solder. Perhaps they can help.
Mike Lamvik

---------------------------------------------------------------------------

John,
We perform analysis of solders quite frequently here at Sandia National
Laboratories. I do not believe that the average or bulk composition of the
solder can be determined by scanning. What we have done with good success is
to
determine the volume fractions of the phases present in the solder and then
determine the composition of each of the phases. At this point it is a fairly
straight forward exercise to calculate the bulk solder composition. Please
contact me if you have any questions.

Joe Michael
jrmicha-at-sandia.gov
-----------------------------------------------------------------------------

John,

We have performed the analyis on our AMRAY 1645 which is equipped with a large
specimen stage and a Noran x-ray system. Our particular application was to
determine the composition of solder as it flowed down a copper conductor on a
printed circuit board. The solders we were interested in were Pb-Sn and I
think
it was 60-40. Anyway, we used the image analysis on the Noran to determine the

area fraction of the two phases on the surface of unpolished solder lines.
Then we very carefully measured the composition of the two phases. We also
made
the assumption that the volume fraction of the two phases was equivalent to the

area fraction that we measured. At this point you need to write a simple
equation that allows you to calculate the bulk composition of the solder from
the volume fractions and the compositions of the phases. You will also need to

know or assume a density for the phases. We published some results from this
technique in Acta Met vol, 43, pp 299-305, 1995 the title was Extensive wetting

due to roughness and was authored by F. G. Yost, J. R. Michael and E. T.
Eisenmann. If you need I will send you a reprint of the paper.

Joe Michael

--------------------------------------------------------------------------


_______________________________________________________________________________

John,
Did you ever get any positive responses. Find anything that works?
John Hunt}
} Hello,
}
} I am interested on the community input on performing in-situ analysis of
solder
} to determine composition. We have tried to do this in the past and have not
} had acceptable results. We used both multiple areas and multiple points and
} averaged the data.
}
} I realize that this is probably an impossible task with EDS, but would like
to
} confirm my opinion and obtain input as to any other techniques (micro probe
} XRF, etc.) which may provide a non-destructive method for in-situ solder
} analysis.
}
} Thanks,
}
} John Giles
} jegiles-at-space.honeywell.com
}


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From: fahayes-at-ucdavis.edu (Fred Hayes)
Date: Wed, 6 Dec 1995 20:35:15 -0800
Subject: non Osmicated retina

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We are contemplating eliminating Osmium Tetroxide as a post fix because of
toxicity and disposal concerns. We are interested in documenting
morphological changes of the rabbit retina as part of a drug toxicity,
efficacy study. Retinas are aldehyde fixed in 2.5% Glutaraldehyde/2.0%
Paraformaldehyde solution, followed by Osmium, ethanol dehydration and epoxy
embedding. 95% of the blocks will be for light evaluation only. 5% will be
selected for TEM low mag wide field. TEM magnification range is 1200-2400X.
No intermediate or higher mag's are ever done.


1) by eliminating OsO4, will the contrast and fixation be adversely affected at
A) the light level?
B) the TEM level (1200-2400x mag)?

if so,

2) is there a suitable alternative to OsO4?

3) at a TEM mag of 1200-2400X, will the differences in contrast and/or
fixation be highly noticeable if OsO4 is eliminated?

sections for TEM are post stained in UA/Pb

Many thanks,

Fred Hayes





From: Self :      RISRMS1/AFM-JQBI
Date: Thu, 7 Dec 1995 09:25:53 MET
Subject: Conference on cement microscopy

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------- Forwarded Message Follows -------

Dear Larry,

The 18th International Conference on Cement Microscopy will take
place in Houston 21-25 April 1996. You can contact Linda Hills,
Construction Technology Labs Inc., 5420 Old Orchard Road, Skokie, IL
60077, USA. Phone: +1 708 965 7500, fax: +1 708 965 6541.

Yours sincerely,

J. B. Bilde-Soerensen
Materials Dept.
Risoe National Laboratory
DK-4000 Roskilde
Denmark.




From: Richard W. Fonda :      fonda-at-anvil.nrl.navy.mil
Date: Thu, 7 Dec 1995 07:12:20 -0600
Subject: x-ray vs. electron diffraction

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While examining the crystallography of the intermetallic TaRu phase, I
discovered that very weak superlattice spots were present in the electron
diffraction patterns which were not observable in x-ray powder diffraction
patterns, even with high resolution and slow scans. I've heard that this is not
uncommon, but was wondering what the reason for this might be. I can imagine a
number of possible contributing factors, including an inherent S/N difference,
orientation effects, and the non-linearity of film exposures, but what is the
best explanation?

Dick Fonda

_____________________________________________________________
Richard W. Fonda Naval Research Laboratory
(202) 767-2622 Code 6324
_____________________________________________________________





From: mary-ellen harper (biochem) :      mharper-at-labsun1.med.uottawa.ca
Date: Thu, 7 Dec 1995 08:13:46 -0500 (EST)
Subject: unsubscribe

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From: Richard E. Edelmann :      edelmare-at-CASMAIL.MUOHIO.EDU
Date: Thu, 7 Dec 1995 08:44:39 -500
Subject: Bellows Source

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I thought others in the group might interested in this as well.
For replacement photographic bellows, if you have no luck finding
OEM bellows, try:

Gortite
A and A Manufacturing Co. Inc.
2300 S. Calhoun Rd.
New Berlin Wisconsin 53151

414-786-1500
fax: 414-786-3280

They have a whole line of 'photographic' bellows (cameras,
enlarger, copy machines, etc.). I just found them, but I haven't
tried them yet. Good luck.


(No financial relationship)

Richard E. Edelmann
Electron Microscopy Facility Supervisor
352 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513-529-5712 Fax: 513-529-4243
E-mail: edelmare-at-muohio.edu
internet




From: Diana_Papoulias-at-nbs.gov (Diana Papoulias)
Date: Thu, 7 Dec 1995 10:13:53 -0700
Subject: LM, specimen adhesion

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Message-Id: {9512071543.AA15489-at-easynet.crl.dec.com}

I am sectioning cod eggs & newly hatched fry embedded in paraffin and
preparing them for the Apop Tag DNA stain. I am using silanized
slides and drying the slides on a slide warmer overnight and I am
still having trouble with losing a significant amount of my specimens
after the Apop procedure. Any advice on improving the adhearance of my
specimens or a way to decrease the loss of my specimens during the
staining procedure would be greatly appreciated.

Contact Diane Nicks at 314 875 5399

or via this e-mail address: Diana_Papoulias-at-nbs.gov




From: agoldsch-at-tamarugo.cec.uchile.cl (GOLDSCHMIDT DE LA MATTA ALFONSO)
Date: Thu, 7 Dec 1995 15:05:06 +0400
Subject: uProbe

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quantitative analysis software , for unix or run on PC ( i need buy)
standard for differents minerals and elements ( Au,Ag, As,Fe) ( i need
analysis result microprobe of differents minerals
i have microprobe cameca MS#46 ( are good) and i need study gold-pip of
river


A.goldschmidt




From: agoldsch-at-tamarugo.cec.uchile.cl (GOLDSCHMIDT DE LA MATTA ALFONSO)
Date: Thu, 7 Dec 1995 15:01:29 +0400
Subject: discussion/comments/questions

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quantitative analysis software , for unix or run on PC ( i need buy)
standard for differents minerals and elements ( Au,Ag, As,Fe) ( i need
analysis result microprobe of differents minerals
i have microprobe cameca MS#46 ( are good) and i need study gold-pip of
river

A.goldschmidt




From: Jay Jerome :      jjerome-at-bgsm.edu
Date: Thu, 7 Dec 1995 09:28:34 -0500 (EST)
Subject: MICROSCOPY FIXATION Re: non Osmicated retina

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We have not specifically looked at retina, but have on occassion left
Osmium out of the soup for various reasons. In general the results are:

1. Reduction in contrast in thin sections (can't comment on thicks) even
with heavy Ua/Pb staining.

2. Very poor preservation of lipids.

We also have concerns about Osmium, but I don't know of any suitable
substitue. I will be interested in other replies.


Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-bgsm.edu *
**************************************************************

On Wed, 6 Dec 1995, Fred Hayes wrote:

} We are contemplating eliminating Osmium Tetroxide as a post fix because of
} toxicity and disposal concerns. We are interested in documenting
} morphological changes of the rabbit retina as part of a drug toxicity,
} efficacy study. Retinas are aldehyde fixed in 2.5% Glutaraldehyde/2.0%
} Paraformaldehyde solution, followed by Osmium, ethanol dehydration and epoxy
} embedding. 95% of the blocks will be for light evaluation only. 5% will be
} selected for TEM low mag wide field. TEM magnification range is 1200-2400X.
} No intermediate or higher mag's are ever done.
}
}
} 1) by eliminating OsO4, will the contrast and fixation be adversely affected at
} A) the light level?
} B) the TEM level (1200-2400x mag)?
}
} if so,
}
} 2) is there a suitable alternative to OsO4?
}
} 3) at a TEM mag of 1200-2400X, will the differences in contrast and/or
} fixation be highly noticeable if OsO4 is eliminated?
}
} sections for TEM are post stained in UA/Pb
}
} Many thanks,
}
} Fred Hayes
}
}




From: mlamvik-at-mcnc.org
Date: Thu, 7 Dec 1995 13:42:06 -0500
Subject: SEM with LaB6 in SE USA

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If anyone has a Hitachi SEM (or Jeol) with a LaB6 source that would be
available for making some comparative pictures, please let me know.
Location in southeastern USA, particularly near Atlanta, is preferred but
not required.
Thanks,
Michael Lamvik
{mlamvik-at-mcnc.org}
MCNC, Research Triangle Park, NC






From: Amy G Aslamkhan :      aslamkha-at-hawaii.edu
Date: Thu, 7 Dec 1995 11:23:56 -1000
Subject: TEM: Looking for Philips 201 TEM parts

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Message-Id: {9512071534.AA15151-at-easynet.crl.dec.com}

We are hoping to find some parts for our Philips 201 TEM. We are
currently in great need of specimen holders (including both injector rods
and tips). We are also low on plate film holders (about 3"x4" in size).
We are interested in finding a 70mm camera hook up for roll film; as now
we are using only plate film which is quite expensive. We would also be
interested in a TV adaptor for the scope. So, if you have these parts
please let us know, and contact me at aslamkha-at-hawaii.edu.




From: William Tivol :      tivol-at-wadsworth.org
Date: Thu, 7 Dec 1995 12:39:37 -0500 (EST)
Subject: Re: x-ray vs. electron diffraction

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} While examining the crystallography of the intermetallic TaRu phase, I
} discovered that very weak superlattice spots were present in the electron
} diffraction patterns which were not observable in x-ray powder diffraction
} patterns, even with high resolution and slow scans. I've heard that this is not
} uncommon, but was wondering what the reason for this might be. I can imagine a
} number of possible contributing factors, including an inherent S/N difference,
} orientation effects, and the non-linearity of film exposures, but what is the
} best explanation?
}
Dear Dick,
I don't know the parameters either of the diffraction set-ups or your
material; however, with those caviats, since ED can use a small number of
unit cells in a selected area, and XD requires more unit cells due to the
lower interaction cross-section, if there are changes in the superlattice
over distances large compared to the selected area but small compared to
the powder grain size, this could account for it. Another possibility is
a change in the structure during the preparation of the powder (unless the
ED was obtained from individual powder grains). Non-linearity of the film
should not affect the presence of the spots--only their intensities. Sat-
uration would, in fact, enhance the fainter spots.
Yours,
Bill Tivol




From: bozzola-at-siu.edu (John. J. Bozzola)
Date: Thu, 7 Dec 1995 17:05:31 -0600
Subject: General: SCSI2 Cabling

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Message-Id: {v01510101aced226420f0-at-[131.230.97.68]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

I need to connect two SCSI-2 cables end to end and am wondering if someone
makes a SCSI-2 adapter to do this? The adapter would have two female
receptacles to accept the male mini 50-pin connectors of the cables. I have
checked all of the usual specialty cable suppliers like Datatek but they
could not help. Suggestions?
Thanks.

#############################################################################
John J. Bozzola, Director
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
#############################################################################






From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 7 Dec 1995 13:09:44 -0400
Subject: RE- Xray vs Electron Diffr

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Message-ID: {n1393780658.73779-at-mse.engin.umich.edu}

Subject: Time: 1:00 PM
OFFICE MEMO RE: Xray vs Electron Diffr Date: 12/7/95

At least part of the reason the superlattice spots show up in the electron
diffraction patterns, but not in x-ray diffraction patterns, stems from the
fact that at small scattering angles the atomic scattering factors for
electrons are several orders of magnitude larger than those for x-rays. This
matter is discussed more fully in a chapter I wrote on electron diffraction
'way back in 1960 in "Physical Methods in Chemical Analysis", W. G. Berl,
Ed. 2nd. edition, p. 620, Academic Press, 1960.
W. C. Bigelow (bigelow-at-umich.edu)





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-aaem.amc.anl.gov
Date: Thu, 7 Dec 1995 10:37:36 -0600 (CST)
Subject: American Association of Feed Microscopists Meeting

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Meeting Name: American Association of Feed Microscopists
Meeting Dates: June 17 - 21 at Iowa City, Ia
Meeting Topic : 43rd Annual Meeting on 17, 18, 19
with topics related to
feed microscopy.

Short Course on 19, 20, 21.
The short course will teach a person how to I.D. feed ingredient
with the microscope. A certificate to those that pass.
Meeting Sponsor: AAFM

Contact Person: Marjorie McCutcheon
P. O. Box 5246
Charleston, W.V. 25361

Telephone Number: 304-558-2208
Fax Number: 304-558-3594
Email Address: r-manuel-at-uiuc.edu








From: Dennis Ahr :      dahr-at-fred.net
Date: Thu, 7 Dec 1995 15:14:23 -0500
Subject: 3D Image Reconstruction SW

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Good afternoon microscopists,

I am looking for a source of commercial software to do 3D image
reconstruction in a work station type environment (Silicon Graphics, Sun,
etc.) using the input from a microscope mounted TV system or, preferably, a
slow scan camera. The application involves the use of biological serial
sections at approximately 10,000x microscope magnification. It has also
been mentioned that the reconstruction could be done using fewer, thicker
sections along with the eucentric tilt of the microscope to provide input to
such a program.

My feeling is that most folks using work stations have devised their own
methods or modified software to suit their unique purposes.

Any suggestions would be appreciated.

Cheers, Voice: 301-696-9074
Dennis Ahr FAX: 301-696-9632
Philips Electronic Instruments E-mail: dahr-at-fred.net





From: Murphy, Judy :      murphy-at-ms.sjdccd.cc.ca.us
Date: 7 Dec 1995 16:32:19 -0800
Subject: NIST X-ray software, address

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Message-ID: {n1393768509.80676-at-ms.sjdccd.cc.ca.us}

I need the address and cost of the NIST EDS software but don't have a phone
number or address. If someone has it please reply to my e mail.
Thanks
Judy Murphy

Judy Murphy, PhD
Dept. of Microscopy
San Joaquin Delta College
5151 Pacific Ave
Stockton, CA 95207

Phone: 209/474-5284
FAX: 209/474-5649
e-mail: murphy-at-ms.sjdccd.cc.ca.us





From: dianavd-at-eye.usyd.edu.au (Diana van Driel)
Date: Fri, 8 Dec 1995 10:50:41 +1100
Subject: Re: non Osmicated retina

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} 1) by eliminating OsO4, will the contrast and fixation be adversely affected at
} A) the light level?
} B) the TEM level (1200-2400x mag)?
}
} 2) is there a suitable alternative to OsO4?
}
} 3) at a TEM mag of 1200-2400X, will the differences in contrast and/or
} fixation be highly noticeable if OsO4 is eliminated?

I sometimes leave out osmium from retina fixation, for LR White embedding.
At the low mag you need fixation defects won't be too obvious, but contrast
will be poor. I have to stain with UAc/PbC for much longer, use a smaller
aperture and print on harder paper. LM isn't affected.


Diana van Driel
Dept Ophthalmology
Sydney University 2006
NSW, AUSTRALIA






From: minter :      minter-at-kodak.com
Date: Thu, 07 Dec 1995 16:47:26 -0500
Subject: Section Collection Loops

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Alternate-Recipient: allowed
Auto-Forwarded: prohibited
Content-Return: allowed
Disclose-Recipients: prohibited
Conversion: allowed
Importance: normal
Priority: normal
Sensitivity: Company-Confidential

Several months ago I received a flyer from one of the supply
houses announcing a new style of section collection loop that
was made by the same process used to make electron microscope
grids. The flyer claimed that the design caused the sections
to concentrate in the center of the loop and aid transfer to
the center of the grid. Now I cannot find the advertisement.

1. Do any of my fellow microscopists remember the advertisement?
Can you point me to the correct vendor?

2. Have any of you used these new loops? Do they perform as
advertised?

To save bandwidth and prevent those "my mailbox runneth over
blues" please respond directly to minter-at-kodak.com and I will
post a summary to the newsgroup.

Thanks.
--
Best Regards,
John

John R. Minter, Ph. D. Phone: (716) 722-3407
Eastman Kodak Company FAX: (716) 477-3029
Analytical Technology Division email: minter-at-kodak.com
Rochester, NY 14562-3712




From: R.G.White-at-sci.monash.edu.au (Rosemary White)
Date: Fri, 08 Dec 1995 09:18:13 +1200
Subject: re: TEM without Osmium

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Dear Fred,

You might try staining with tannic acid/ferric chloride, which we have used
as an alternative to OsO4 to preserve antigenicity, etc. TA is added to
the fix (must be Na-PIPES or NaPO4 buffer not K or TA precipitates) then
you postfix with the same concentration of aqueous FeCl3 (no buffer). This
is described in a paper by Overall et al. 1982 Protoplasma 111:134 (used 2%
TA in glut/form fix followed by 2% FeCl3, each for 2h), in which older
references are cited. Have no idea if it would work well with animal
tissues but it brings out all plant membranes and other cell components
(i.e. ones that we're interested in!) very well. I haven't used OsO4 for
years. We now use only 0.5% TA and FeCl3. If you add up to 10% DMSO to
the fixative, penetration is very good (may not do much for membranes, but
we're only after the cytoskeleton). UA/Pb staining of sections is fine.
More info on TA is given in Mollenhauer and Morre 1987 Histochemistry 88:17

For some immunoEM work I don't use any postfix at all - membranes are fuzzy
but UA/Pb stain after gold labelling is OK as long as the Reynolds Pb is at
the correct pH.

Hope this helps,
Rosemary WHite



____________________________________________________________
Rosemary White __ /
Department of Ecology _/ \__/ \
and Evolutionary Biology / \
Monash University / Australia \
Clayton, Victoria 3168 \ ____ /
phone 61-3-9905 5670 \_/ \_*_/
fax 61-3-9905 5613 __
email r.g.white-at-sci.monash.edu.au \/






From: GVKM07A-at-PRODIGY.COM (DR CHARLES A GARBER)
Date: Thu, 07 Dec 1995 17:28:01 EST
Subject: Carbon paint purity

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-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --

There has been some recent discussion about carbon paint used for SEM
sample prep:

Make sure it's pure graphite! I've found carbon paints sold for
mounting specimens for EDX that are contaminated with phosphorus . Phil
Oshel

This is a good example of seemingly similar products not being so
similar. If you compare the EDS spectra from a "dab" of the SPI Carbon
paint on a spectroscopically pure carbon SEM mount vs. the carbon mount
only control spectra, by stretching the imagination, one might see a
bit of an Si peak in both scans and in addition, on the carbon paint
scan, but not on the control, you again, by really stretching it, some
claim to "see" a very tiny indication of Cl. Other than that the paint
is "clean". This to me is the acid test of purity.

We have tested other carbon paints in our laboratory over the years.
All paints are most definitely not created equal when subjected to the
above quality test and there is some variability in purity and also,
quality of the brush/cap assembly (which can also release unwanted
elements)..

I would be happy to send copies of the original data should anyone want
to receive a copy. Send your FAX number with your request.

Chuck

======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail:
GVKM07A-at-prodigy.com
West Chester, PA 19381-0656 USA Customer Service:
SpiSupp-at-aol.com

########################################################
WWW: http://mail.cccbi.chester.pa.us/spi/spihome.html
########################################################
======================================================





From: zaluzec-at-Microscopy.Com (Nestor J. Zaluzec)
Date: Thu, 7 Dec 1995 19:16:22 -0600
Subject: INTAS-RFBR project a request from Moscow on A3B5's semiconductors

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G'day Colleagues...

I have received a request from Professor S.Maksimov of the
Laboratory of electron microscope investigations at Moscow Institute of
Electronic Technology
Russia (E-mail: lemi-at-mx.iki.rssi.ru) to post a long message to the listserve=
r.

Basically his group is looking for collaborators (and sources of funding
for a project they are working on). on A3B5 semiconductor compounds.

As a favor, I have edited their long text message and attached an
abbreviated copy herein.
If anyone is interested in this project or knows of someone that might be
can you pass this along. If you want the longer version I'm sure he will
be happy to send it to you via Email.

=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=
=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=
=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D



}
} Dear Doctor Nestor Zaluzec.

} Please, could You study our information and if it is possible
} place it on Microscopy Listserver or give us a council concerning a
} place of its location.
}
} New Mechanism of Dissipative Structure Formation in A(3)B(5).
}
} We are looking for partners in investigations of regularities of
} non-equilibrium ordering (atomic ordering and composition automodula-
} tion) in epitaxial layers of the A3B5 compositions: GaAlAs, GaAsP, etc.
} We have carried out such investigations beginning with 1978.(S.K.
} Maksimov, E.N.Nagdaev, Doklady Akademii Nauk SSSR, (in Russian) 1979,
} 245, 1369).
} We are interested in receiving of the financial support of the
} scientific project from international organization for continuation of
} investigations what we have carried out recently (really without any
} financial support) and in which we obtained the results allowing to
} examine the problem in the new light. At present we have finished the
} interpretation of part of these results and prepare them for a publi-
} cation (see below the short description of these results).
} We are trying to get the financial support in the frames of the
} joint research projects of INTAS and RFBR (but, of course we will be
} glad any other possibility of a support) and for these purposes are
} looking for two research groups in West Europe, which would be
} interested in mutual investigations and could participate in them.
} Unfortunately,the dead-line of the presentation of the project is 15
} of December.
}
} Information via Internet
}
} The information package is also available via:
} + World Wide Web on the following sites through CORDIS (the
} Community R&D Information Service), INTAS and RFBR:
} o CORDIS ([8]http://www.cordis.lu/cordis/)
} o INTAS ([9]http://www.ib.be/intas/)
} o RFBR ([10]http://www.rfbr.ru/kon/intas/toc.en.html)
}
} The idea of these investigations (at least, in the theoretical
} aspect) arose during the conference "Microscopy of Semiconductor Mate-
} rials" which took place at Oxford University on 25-28 March 1991, but
} absent of a financial support dragged out its realization.



} Our results. (THIS PART IS EDITED FROM THE ORIGINAL)



} We studied structure and physical properties of GaAlAs epitaxial
} layers with a help of techniques: TEM, HREM, TED, reflection ED, ca-
} thodoluminescence, etc. In Ga(1-x)Al(x)As/(001)GaAs produced by the
} MOCVD technique two types of the dissipative structures competes: the
} atomic ordering can be changed by the composition automodulation. At x
} =3D 0.2 the ordering direction coincide with the growth direction, but
} at x =DA 0.3 it is changed by more traditional that: [010] lying in the
} surface plane. Transition from the ordering to the modulation is pro-
} voked by breaking of the layer growth mechanism and transition to the
} many-center origin (this type of the origin stimulated an acceleration
} of the growth). Modulation direction is perpendicular or incline to
} the growth direction and does not correspond to models of dissipative
} structure formation. Specimens keeping the atomic ordering have only
} one line of cathodoluminescence corresponding to an average compositi-
} on of the layer but for Ga(0.7)Al(0.3)As (for which structural inves-
} tigations showed existence of the modulation)the lines corresponding
} to GaAs, Ga(0.3)Al(0.7)As and Ga(0.4)Al(0.6)As are observed. Existence
} of modulation is the indubitable phenomenon but its specific features
} demands an explanation. Besides the theoretical analysis demonstrates
} that an existence of the modulation leads possibly to corrections of
} mechanisms of the atomic ordering.
} The same dissipative structures are observed for different
} technologies and materials and this fact has led us to the idea that a
} reason of the non-equilibrium phase transformations must be
} fundamental and characteristic for the all A(3)B(5) compounds and does
} not connect with separate technologies.


Lots of supporting TEXT DELETED
you may get the rest from Maksimov
at the address listed below.




} Professor S.Maksimov
}
} Laboratory of electron microscope investigations
} Moscow Institute of Electronic Technology
} Russia
} E-mail: lemi-at-mx.iki.rssi.ru
}
}
}






From: MicroToday-at-aol.com
Date: Fri, 8 Dec 1995 06:54:43 -0500
Subject: Oxford Acquires Microspec

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The Oxford Instruments Microanalysis Group has just acquired the
Microspec Company in Fremont, CA, adding Microspec's WDX400/600 wavelength
dispersive (WDX) spectrometers to its range of Link products and systems.
There will be no change in the management or staff at Microspec, and as
Richard Wolf, President of Microspec, points out to their customers "Only the
company name will change".
Don Grimes, Microscopy Today




From: vicenzi-at-phoenix.princeton.edu (Ed Vicenzi)
Date: Fri, 8 Dec 1995 10:08:05 -0500
Subject: Re: NIST X-ray software, address

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Message-Id: {v01510103acee045d5e43-at-[128.112.128.184]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

} I need the address and cost of the NIST EDS software but don't have a phone
} number or address. If someone has it please reply to my e mail.
} Thanks
} Judy Murphy

The person in charge of DeskTop Spectrum Analyzer (DTSA) at NIST is Bob
Myklebust. The cost is $815 for version 2.0. Call (301) 975-2208 for more
information.

Ed Vicenzi tel (609) 258-1464 office
Princeton University tel (609) 258-1406 lab
Princeton Materials Inst. fax (609) 258-6878
70 Prospect Ave.
Princeton, N.J.
08540-5211 vicenzi-at-princeton.edu






From: John G Humenansky :      humen001-at-maroon.tc.umn.edu
Date: Fri, 8 Dec 1995 08:23:23 -0600 (CST)
Subject: subscribe to newsgroup

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please subscribe

Thanks




From: Karen L. Vaughn :      klv-at-biotech.ufl.edu
Date: Fri, 8 Dec 1995 11:15:49 GMT
Subject: TEM screen

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We are looking for an alternate company to coat fluorescent TEM screens. We
have used Grant Scientific in the past with somewhat satisfactory results
but find that the recoated surface does not last. Is there another company
or EM supplier that coats TEM screens?

Thanks in advance


----------------------------------------------------------------------------
---------
Karen Vaughn Tel.(904) 392-1184
EM Technician
University of Florida Fax.(904) 846-0251
Electron Microscopy Core Laboratory Email. KLV-at-biotech.ufl.edu
Interdisciplinary Center for Biotechnology Research
http://www.biotech.ufl.edu/~emcl/
214 Bartram Hall
Gainesville, Fl 32611







From: tphillips-at-biosci.mbp.missouri.edu (Tom Phillips)
Date: Fri, 8 Dec 1995 10:49:11 -0500
Subject: LM & EM localization of oxidases generating peroxides

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Message-Id: {v01510102acee0dd11935-at-[128.206.15.189]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Does any one have a good protocol or references to techniques localizing
oxidases that generate peroxides at the light or electron microscopic
level? Thanks in advance.


Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility
3 Tucker Hall
University of Missouri
Columbia, MO 65211
(314)-882-4712 (voice)
(314)-882-0123 (fax)






From: Laurent Bataillard :      bad-at-igasg1.epfl.ch
Date: Fri, 8 Dec 1995 18:22:19 +0100
Subject: unsuscribe

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Please unsuscribe

--
Laurent Bataillard
IGA - DP
EPFL - 1015 Lausanne

phone (41)(21) 693.44.73
fax (41)(21) 693.444.70
bad-at-igasg1.epfl.ch




From: bergrh-at-msuvx2.memphis.edu (R. Howard Berg)
Date: Fri, 08 Dec 1995 10:23:03 +0600
Subject: Re: General: SCSI2 Cabling

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Try APS Technologies, 800-304-9057
They have a variety of SCSI products and have very good service support.

} I need to connect two SCSI-2 cables end to end and am wondering if someone
} makes a SCSI-2 adapter to do this? The adapter would have two female
} receptacles to accept the male mini 50-pin connectors of the cables. I have
} checked all of the usual specialty cable suppliers like Datatek but they
} could not help. Suggestions?
} Thanks.


R. Howard Berg, Ph.D.
Biology Department
University of Memphis
Memphis, TN, 38152
E mail: bergrh-at-cc.memphis.edu
phone: 901-678-4449 fax: 901-678-4457







From: Myers.S/Apple-at-eworld.com
Date: Fri, 8 Dec 1995 09:33:50 -0800
Subject: Re: x-ray vs. electron diffraction

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Message-Id: {199512081615.LAA25190-at-thomas.ge.com}
X-Authentication-Warning: thomas.ge.com: daemon owned process doing -bs

I had a similar experience with intermetallic Fe3Al-C prepared by rapid
solidification techniques. The samples had an FCC structure in X-ray
diffraction but the electron diffraction patterns had weak superlattice
reflections. In my case, when the superlattice reflections were used to
form a dark field image, small areas within the matrix were imaged. These
areas were an ordered phase in a disordered matrix. With different
solidification rates, the intensity of the superlattice reflections changed
and the volume fraction of ordered regions correspondingly increased or
decreased.* I had a lot of help from Joe Horton at Oak Ridge National lab
who is an intermetallic, TEM, X-ray wizard.
Good luck,
Sharon

*S.A. Myers and C.C. Koch, "Electron Diffraction Structure Analysis of
Rapidly Solidified (Fe,Ni)3Al-C Alloys",Ultramicroscopy 30 (1989) 193.




From: Murphy, Judy :      murphy-at-ms.sjdccd.cc.ca.us
Date: 8 Dec 1995 13:41:32 -0800
Subject: NIST address,Thank You

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Message-ID: {n1393692402.60985-at-ms.sjdccd.cc.ca.us}

Thank you all very much. I needed it very quickly and got the info in a very
timely fashion. To respond to someone's comment, I did call NIST but didn't
hit the right place.
Thanks again,
Happy Holidays,
Judy Murphy




From: FNKPS-at-AURORA.ALASKA.EDU (Ken Severin)
Date: Fri, 8 Dec 1995 09:42:47 -1000
Subject: probe software

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X-Sender: fnkps-at-galileo.uafadm.alaska.edu
Message-Id: {v01510100acee44815a06-at-[137.229.52.74]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Dear Dr. Goldschmidt:

Are you familiar with the large package of PC software that came out of
Caltech (mostly from John Armstrong?) known as CITZAF? It would do what you
need, but is not set up for automated work. It can do just about any kind
of ZAF correction as well as Bence-Albe, and it is freely available. Let
me know and I can send you a copy. Best, Ken Severin

Ken Severin Voice: 907-474-5821
Geology and Geophysics
324 Natural Science Bld. Fax: 907-474-5163
Univ Alaska Fairbanks
Box 755780 INTERNET:FNKPS-at-AURORA.ALASKA.EDU
Fairbanks AK 99775-5780






From: MELLIOTT-at-prl.pulmonary.ubc.ca
Date: Fri, 8 Dec 1995 15:10:15 +0800PST
Subject: JB-4 Specimen Stubs

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JB-4 Specimens Using Metal or Plastic Stubs

We have 2 labs in our hospital that process tissue into methacrylate.
One lab uses plastic stubs and a metal adaptor to fit them into the
JB-4 microtome. They often experience problems when cutting, the
blocks cannot be held firmly enough, resulting in uneven sectioning.
The other lab recycles the aluminum stubs, a time consuming task; we
use a hammer and single sided razor blade to chop off the specimens
for storage and then soak the stubs in water until the methacrylate
is soft enough to remove.
Has anyone any suggestions for improving:
1) the cutting of sections using the plastic stubs?
2) the method for recycling the aluminum ones????

Thanks in advance

Mark Elliott, PhD
UBC-Pulmonary Research Lab
Vancouver, BC
Canada




From: William Tivol :      tivol-at-wadsworth.org
Date: Fri, 8 Dec 1995 14:14:35 -0500 (EST)
Subject: Re: 3D Image Reconstruction SW

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Message-ID: {8EA1C74001D90C00-at-cea.com}

} I am looking for a source of commercial software to do 3D image
} reconstruction in a work station type environment (Silicon Graphics, Sun,
} etc.) using the input from a microscope mounted TV system or, preferably, a
} slow scan camera. The application involves the use of biological serial
} sections at approximately 10,000x microscope magnification. It has also
} been mentioned that the reconstruction could be done using fewer, thicker
} sections along with the eucentric tilt of the microscope to provide input to
} such a program.
}
Dear Dennis,
If you contact Joachim Frank, he can tell you about the avail-
ability of the SPIDER image processing system. I think it can meet your
needs since we use it here to do 3D reconstruction by serial sections,
thich-section contouring, and other methods. Both Fourier methods and
weighted back-projection are used, and projection onto convex sets is
also. Once you have a computer file, the program doesn't care whether
the data are from film, TV or SSCCD; you will, of course, have to provide
the frame-grabber or storage buffer which converts the video image into
a digitized file. You can contact Joachim Frank at joachim-at-wadsworth.org
or look at the web page "http://www.wadsworth.org".
Yours,
Bill Tivol




From: NAME Robert Josephs\\ :      bob-at-befvax.uchicago.edu -at-Sparc5.Microscopy.Com
Date: Sat, 09 Dec 1995 15:12:00 EST
Subject: Re: 3D Image Reconstruction SW

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Sender: bob-at-befvax.uchicago.edu

aaa
test





From: dietmar.reiter-at-uibk.ac.at (Dietmar Reiter)
Date: Mon, 11 Dec 1995 09:16:08 +0100
Subject: Re: 3D Image Reconstruction SW

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} I am looking for a source of commercial software to do 3D image
} reconstruction in a work station type environment (Silicon Graphics, Sun,
} etc.) using the input from a microscope mounted TV system or, preferably, a
} slow scan camera. The application involves the use of biological serial
} sections at approximately 10,000x microscope magnification. It has also
} been mentioned that the reconstruction could be done using fewer, thicker
} sections along with the eucentric tilt of the microscope to provide input to
} such a program.

The following is NOT a commercial.

If you accept manual tracing of interesting structures on an overlay-layer
of imported tif images, you might use 3D - T.O.P. XW (of IMATEC, Neufahrn,
Germany) running on SGI (IRIX 5.3). It has powerful interactive
visualization capabilites (realtime rotation and zooming in wireframe
mode), good reconstruction and shading caps, but so far rudimentary import
caps only. A new tracer/import module for different file format import (and
autotrace features) is announced by early 1996.

For information contact:

IMATEK
Elektronische Bildanalysesysteme GmbH
Zugspitzweg 12, D-85375 Neufahrn, Germany

phone: (+49)-8165-65882
fax: (+49)-8165-62750



+++ Dietmar Reiter {dietmar.reiter-at-uibk.ac.at} ++++++++++++++++
+++ Dept. of Zoology and Limnology, University of Innsbruck ++++
+++ Technikerstrasse 25, A - 6020 Innsbruck, Austria +++++++++
+++ ph: (+43)-512-507-6170 (-6161), fax: ..-507-2930 (-2957) +++






From: Vibrotechnika :      vibro-at-vb.ktu.lt
Date: Mon, 11 Dec 1995 15:12:58 +0000 (MET)
Subject: SPM (fwd)

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Hi,

I am PhD student at Kaunas University of Technology. Currently I am
working in Research Centre "Vibrotechnika". Our research work cover
acoustic waves investigation. We are looking for some low priced Atomic
Force Microscope for further investigations. This microscope can be used
and not new. I will be really thankful to everybody who will send me any
proposal.

Address
Mindaugas Rackaitis
Mickeviciaus 11
3000 Kaunas, Lithuania
e-mail: vibro-at-vb.ktu.lt






From: leeman-at-VOEDING.TNO.NL
Date: Mon, 11 Dec 1995 16:26:57 EST
Subject: urgent: HBO-50 lamp

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Dear microscopists,

Which laboratory in the Netherlands can help me? I am urgently looking for
a HBO-50W/3 DC lamp. The supplier needs approx. 4 weeks before they can
deliver the lamp while we are using the lamp for 40 hrs a week. Can we buy
one from you, or borrow, please contact me.

Winfried Leeman
TNO Nutrition and Food Research Institute
Toxicology Division
Phone (+31) 030 - 694 41 44
Direct phone (+31) 030 -694 44 97






From: Kathy Walters :      kwalters-at-emiris.iaf.uiowa.edu
Date: Mon, 11 Dec 1995 11:11:17 -0600 (CST)
Subject: Re: JB-4 Specimen Stubs

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Dear Mark,

I have used JB-4 quite a bit and also (due to the high cost of the metal
chucks), switched to the plastic ones. I have really not had too much
dificulty in making the switch,although I have occationally used a pair of
pliars to secure them into the metal adaptors.

The best way to recycle the aluminuim ones is to secure them in a vise,
and use a hammer and straight screwdriver at a 45 degree angle at the
base. (be sure and use eye protection) This method also relieves
frustration.

Good Luck,
Kathy Walters
U of Iowa


On Fri, 8 Dec 1995 MELLIOTT-at-prl.pulmonary.ubc.ca wrote:

} JB-4 Specimens Using Metal or Plastic Stubs
}
} We have 2 labs in our hospital that process tissue into methacrylate.
} One lab uses plastic stubs and a metal adaptor to fit them into the
} JB-4 microtome. They often experience problems when cutting, the
} blocks cannot be held firmly enough, resulting in uneven sectioning.
} The other lab recycles the aluminum stubs, a time consuming task; we
} use a hammer and single sided razor blade to chop off the specimens
} for storage and then soak the stubs in water until the methacrylate
} is soft enough to remove.
} Has anyone any suggestions for improving:
} 1) the cutting of sections using the plastic stubs?
} 2) the method for recycling the aluminum ones????
}
} Thanks in advance
}
} Mark Elliott, PhD
} UBC-Pulmonary Research Lab
} Vancouver, BC
} Canada






From: jeremy.Sanderson-at-path.ox.ac.uk :      sanderson-at-molbiol.ox.ac.uk
Date: Mon, 11 Dec 1995 16:54:48 0000
Subject: LM equipment wanted

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Dear all

I am looking for a surplus dual observation head and Nomarski DIC equipment for teaching the
principles of microscopy -is anyone able to help me?

TIA Jeremy.




From: (colleen ann lavin) :      lavinca-at-vms2.macc.wisc.edu
Date: Mon, 11 Dec 1995 13:57:49 -0600
Subject: Balzer parts: CPD 010

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Message-Id: {v02120c10acf23c90e5e5-at-[144.92.132.27]}



George Smith from Union College asks for help to refurbish his critical
point dryer. He has a Balzers CDP 010. It has a small leak in the line
somewhere, plus the top
of the bomb is busted and needs to be replaced or repaired.
In addition, George is looking for an oil diffusion pump for a Hitachi
Vacuum evaporator. If you have any leads contact:

George Smith
Biology
Union College
Schenectady, NY 12308
(518) 388-6241

smithg-at-gar.union.edu


====================================================

Colleen A. Lavin
Integrated Microscopy Resource
High Pressure Freezer Coordinator
Madison, WI 53706
608-263-8481
608-265-4076 fax
lavinca-at-macc.wisc.edu
http://www.bocklabs.wisc.edu/imr/imr.html

IMR Symposium Sept. 20-22, 1996






From: dbf-at-fullam.com (Dianne Fullam)
Date: Mon, 11 Dec 1995 15:56:19 -0500 (EST)
Subject: TEM Screen Recoating

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In response to klv-at-biotech.ufl.edu (Karen L Vaughn), seeking a company that
recoats fluorescent TEM screens, Ernest F. Fullam, Inc does TEM screen
recoating and has for many years. Please contact us for pricing and more
information.
Dianne Fullam
Ernest F. Fullam, Inc.
Phone: (518) 785-5533 FAX: (518) 785-8647
E-Mail: dbf-at-fullam.com





From: Glen Macdonald :      glenmac-at-u.washington.edu
Date: Mon, 11 Dec 1995 11:56:51 -0800 (PST)
Subject: Re: JB-4 Specimen Stubs

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X-Sender: glenmac-at-homer23.u.washington.edu

If you have enough of the metal stubs, don't bother breaking off the
methacrylate. Just hold them in water for a week or so, then cut off the
block. Then soak some more until you can push out the 'tail'.

Regards,
Glen

On Fri, 8 Dec 1995 MELLIOTT-at-prl.pulmonary.ubc.ca wrote:

} JB-4 Specimens Using Metal or Plastic Stubs
}
} We have 2 labs in our hospital that process tissue into methacrylate.
} One lab uses plastic stubs and a metal adaptor to fit them into the
} JB-4 microtome. They often experience problems when cutting, the
} blocks cannot be held firmly enough, resulting in uneven sectioning.
} The other lab recycles the aluminum stubs, a time consuming task; we
} use a hammer and single sided razor blade to chop off the specimens
} for storage and then soak the stubs in water until the methacrylate
} is soft enough to remove.
} Has anyone any suggestions for improving:
} 1) the cutting of sections using the plastic stubs?
} 2) the method for recycling the aluminum ones????
}
} Thanks in advance
}
} Mark Elliott, PhD
} UBC-Pulmonary Research Lab
} Vancouver, BC
} Canada
}




From: Mike Folsom :      mwfolsom-at-unm.edu
Date: Mon, 11 Dec 1995 11:36:19 -0700 (MST)
Subject: Re: General: SCSI2 Cabling

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On Thu, 7 Dec 1995, John. J. Bozzola wrote:

} I need to connect two SCSI-2 cables end to end and am wondering if someone
} makes a SCSI-2 adapter to do this? The adapter would have two female
} receptacles to accept the male mini 50-pin connectors of the cables. I have
} checked all of the usual specialty cable suppliers like Datatek but they
} could not help. Suggestions?
} Thanks.
}
} #############################################################################
} John J. Bozzola, Director
} Center for Electron Microscopy
} Southern Illinois University
} Carbondale, IL 62901-4402
} U.S.A.
} Phone: 618-453-3730
} Fax: 618-453-2665
} Email: bozzola-at-siu.edu
} Web: http://www.siu.edu/departments/shops/cem.html
} #############################################################################
}
}
}

John -


Based on painful personal experiences you may not want to do this. First
there is a max length for your old run-of-the-mill SCSI-2 cables, its
about 6 ft. If the combined cables that make up a classic SCSI-2 chain
are longer than that you might experience problems.

My advice is to find a cable that has the correct ends and is as short as
possible. Don't try to save a few $'s here, it just ain't worth it!


M-


______________________________________________________________________________
M.W.Folsom/Biology/UNM/Albuquerque,NM~87131/505.277.2717/mwfolsom-at-mail.unm.edu





From: Dennis Ahr :      dahr-at-fred.net
Date: Mon, 11 Dec 1995 14:55:46 -0500
Subject: 3D Image Recon -- A Thank You & Summary

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Thanks to everyone who responded to my request for information regarding 3D
Image Reconstruction Software for Workstations. I have included all the
responses I have received thus far (with minimal editing). Each response is
separated with a line. Other interested parties might find something useful
here.

Regards,
Dennis

Summary follows:

NASA's biotechnology division released a 3D recon. package specifically for
EM and confocal work about 3 or 4 months ago. It was for US use only and
was apparently available free of charge if you could justify its use (and
were a US citizen). Perhaps you could contact them regarding this. It was
" advertised" on the WWW NASA pg.

Universal Imaging have a package called "metamorph" that I have'nt looked at
(asked for more info. and did'nt get a response) that claims to do 3D recon.
as well as photomontage work that can integrate images from a CCD camera.
It supposedly has a number of other abilities and is available from
Universal Imaging Corp., 502 Brandywine Parkway, West Chester, PA 19380.

There is also a SW package called VoxalView (sp.) from Vital Images that
does 3D recon. It is, however, quite expensive (} $10K). I've asked around
about it and been told it is not so good for some specific applications.

We currently have a package that has been custom designed for our purposes
(3D recon. from 35 mm EM negs. with automated alignment to do volume and
area measurements of smooth muscle cells and associated nerves). We have'nt
gotten as far as publishing with it yet, however when we have we intend to
have it available for general distribution and use. According to the guy
thats developing the program there are a number of useful bits on the WWW,
but I think that's for people who have the time and know how to find them
(as I certainly would'nt know where to start).

Regards,

Shaun Sandow,
Division of Neuroscience,
John Curtin School of Medical Research,
Australian National University,
ACT 0200

Ph. (06) 249 4782
Fax. (06) 249 2687
_________________________________________________________________

I have been associated with a couple labs trying to solve this problem, and
we at NRL have also investigated the problem for an upcoming program. In a
nutshell, there do exist commercial programs out there, particularly geared
towards the medical community. However, they typically have a low
resolution and cannot handle the more complex structures often seen in
materials science. In every case I know of, this has led to (at least an
attempted) development of
the actual software. Unfortunately, the research group which appeared to be
heading this effort, at least in the area of solid-solid phase
transformations (headed by Gary Shiflet of UVa) just lost much of their
computer support and no longer has a feasible system (as far as I know). We
will be working to develop our own system probably within the next 1/2 year
because of this.

For the application you describe, the commercially available software may
have sufficient resolution. We have not checked out that software, but Dr.
Shiflet did before he started developing their own system. You might want
to contact him at gjs-at-gjssgi.ms.virginia.edu for further information.

Dick Fonda [Naval Research Lab, Washington, DC]
_______________________________________________________________________

VayTek, Inc.
305 West Lowe, Suite 109
P.O. Box 732
Fairfield, Iowa 52556
515-472-2227
FAX: 515-472-8131

Christopher MacLean, Ph.D.

vaytek-at-ins.infonet.net

Software for PC's (DOS/Windows 3.11, and Windows NT), Unix, and Mac's.
Their main software for 3D reconstruction is called VoxBlast, they also have
assisant software for aligning 2D sections, and they have deconvolution
microscope controlling software as well. All of
which is designed to be intergrated with each other and Image Pro Plus.

I have tried their demo software (available on line at ftp://128.255.88.195,
User: vbdemo, password: 5UZY2fCn [case sensitive]), and I have had demos
done for me but I have yet had the
funding to get my own copy of the software. The technical support and sales
reps are friendly, knowledgable, and very willing to help.

A second set of software for Unix (and PC's?) is VoxelView, but I do not
have the contact info available for them.

Richard E. Edelmann
Electron Microscopy Facility Supervisor
352 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513-529-5712 Fax: 513-529-4243
E-mail: edelmare-at-muohio.edu
_______________________________________________________________________

I am using Spyglass, which is now distrubuted by Fortner Research (tel: 800
252-6479 or http://www.langsys.com/langsys.

I am analyzing serial sections obtained by SEM and run the application on a
Mac (good enough for this purpose). However, Spyglass is also available for
UNIX platforms. Excellent software and easy to use (I never used a manual).
I typically have 20 sections, each 640*512 pixels and they are rendered
rapidly.This software is only for data reprssentation and not for data
anaysis. It would be desirable to be able to extract stereological features
from any 3D data set, however, this is not build in.

I hope that this helps. Please let me know if you come across other sources
for this applications.

Hasso Weiland
Senior Scientist
Aluminum Company of America
Alcoa Technical Center
Alcoa Center, PA 15069
412-337-3133
weiland_h-at-atc.alcoa.com
______________________________________________________________________

Have you seen Voxel View? We used it at Texas A&M to reconstruct confocal
sections on a SGI system. I think it would fit your app, but like most
workstation software - it's expensive.

Good Luck,

James C. Long
Manager/Materials Analysis Lab
Electrosource, Inc.
512-445-6606
jlong-at-bga.com
_______________________________________________________________________

Try the John Steven Groug at Playfair in Toronto.

Contact Judy Trogadis judy-at-CAMTWH.ERIC.ON.CA or John, john-at-camtwh.eric.on.ca

He has written the book on this type of thing which is very different that
using confocal input data because TEM sections get distorted and misaligned
before they are recorded.

Prof. James B. Pawley, Ph. 608-263-3147
Room 1235, Engineering Research Building, FAX 608-265-5315
1500 Johnson Dr., Madison, WI, 53706 JBPAWLEY-at-FACSTAFF.WISC.EDU
_____________________________________________________________________

If you contact Joachim Frank, he can tell you about the availability of the
SPIDER image processing system. I think it can meet your needs since we use
it here to do 3D reconstruction by serial sections, thich-section
contouring, and other methods. Both Fourier methods and
weighted back-projection are used, and projection onto convex sets is also.
Once you have a computer file, the program doesn't care whether the data are
from film, TV or SSCCD; you will, of course, have to provide the
frame-grabber or storage buffer which converts the video image into a
digitized file. You can contact Joachim Frank at joachim-at-wadsworth.org or
look at the web page "http://www.wadsworth.org".
Yours, Bill Tivol
________________________________________________________________________

Your're right-most individuals with workstations have developed their own
custom software. Jill Gemmill, at the Neurobiology Research Center at
University of Alabama at Birmingham, Birmingham, Al (205-934-7111) developed
an excellent 3-D reconstruction package on a Silicon Graphics Workstation.
The software was used to perform three dimensional serial reconstruction
from images generated by photographs of serial sections in an electron
microscope. Granted the interface to capture images directly to the
workstation via slow scan camera is not there, but the reconstruction
software that Jill developed is super. I cannot find an E-mail address for
her, but above is a direct line to her facility. If anything, she might be
able to help you out a bit.

Best Regards

Kevin
Kevin McCarthy
Assistant Professor
Department of Cell Biology
Digital Imaging Microscopy Facility
University of Alabama at Birmingham
Birmingham, Alabama 35294
Phone 205-934-9923/9924
Fax 205-934-7029
"Seeing the World Through Different Eyes"
________________________________________________________________________

Mayo Clinic puts out a software package that can do almost anything you
want it to, however the onsite cost is around $60,000.00.

I have a friend and College at our Beckman Research Institute that is going
to teach this software to me. Her name is Janet Sinn-Hanlon, and her email
address is:

Janet-at-delphi.beckman.uiuc.edu

* Janet works only part time, so it may take her a few days to get back to
you. Tell her I sent you, she would be glad to discuss the software with
you. It could be an individual purchase would be much less expensive.

Good Luck,

Lou Ann Miller
Microscopic Imaging Lab
College of Vet. Medicine
University of Illinois
2001 S Lincoln Ave
Urbana,Illinois 61801
217-244-1566
lmiller-at-ux1.cso.uiuc.edu

Microscopy Home Page:
http://www.cvm.uiuc.edu/MicImagLab/MicImagLab.html

Personal Home Page:
http://www.cvm.uiuc.edu/HomePages/LouAnnMiller/Homepage.html
________________________________________________________________________

The following is NOT a commercial.

If you accept manual tracing of interesting structures on an overlay-layer
of imported tif images, you might use 3D - T.O.P. XW (of IMATEC, Neufahrn,
Germany) running on SGI (IRIX 5.3). It has powerful interactive
visualization capabilites (realtime rotation and zooming in wireframe
mode), good reconstruction and shading caps, but so far rudimentary import
caps only. A new tracer/import module for different file format import (and
autotrace features) is announced by early 1996.

For information contact:

IMATEK
Elektronische Bildanalysesysteme GmbH
Zugspitzweg 12, D-85375 Neufahrn, Germany

phone: (+49)-8165-65882
fax: (+49)-8165-62750

+++ Dietmar Reiter {dietmar.reiter-at-uibk.ac.at} ++++++++++++++++
+++ Dept. of Zoology and Limnology, University of Innsbruck ++++
+++ Technikerstrasse 25, A - 6020 Innsbruck, Austria +++++++++
+++ ph: (+43)-512-507-6170 (-6161), fax: ..-507-2930 (-2957) +++





From: Paul.Fischione-at-internetmci.com
Date: Mon, 11 Dec 1995 20:44:33 -0500
Subject: Job Opening - Machinist/Instrument Maker

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-- [ From: Paul E. Fischione * EMC.Ver #2.3 ] --

E.A. Fischione Instruments, Inc is seeking a highly skilled and motivated
individual for a senior machinist/instrument maker position. Duties will
include working with the engineering staff throughout the design and
development of innovative electron microscopy accessories including the
fabrication and intricate assembly of ultra-precise mechanical/electro-
mechanical components. Additional activity will be in the support of
production utilizing state-of-the-art CNC turning and vertical machining
centers. The candidate must be able to read engineering drawings.
Knowledge of manufacturing practices for vacuum usage is a plus.

E.A. Fischione Instruments, Inc. was founded in 1966 and specializes in the
development and manufacture of TEM specimen preparation devices. The
company is located in Export, PA, approximately 23 miles east of the city
of Pittsburgh.

Resumes should be sent to: Paul E. Fischione, President
E.A. Fischione Instruments, Inc.
9003 Corporate Circle
Export, PA 15632




From: Donald P Robertson :      donald-at-csd.uwm.edu
Date: Mon, 11 Dec 1995 20:01:27 -0600 (CST)
Subject: subscribe to newsgroup

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subscribe Donald P. Robertson {Donald-at-csd.uwm.edu}




From: SBDX78A-at-PRODIGY.COM ( KATY T RUSHNOV)
Date: Mon, 11 Dec 1995 21:54:53 EST
Subject: does this work

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i'm checking to see if this is received





From: richard.easingwood-at-stonebow.otago.ac.nz (Richard Easingwood)
Date: Tue, 12 Dec 1995 17:24:08 +1100
Subject: TEM: Artifacts(?) with Imidazole/OsO4 Fixation

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X-Sender: st004718-at-brandywine.otago.ac.nz
Message-Id: {v01530501acf2cf6331c3-at-[139.80.120.143]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Richard Lander asks:

Has anyone had dealings with Imidazole + Osmium tetroxide post fixation
methods.... And if they have, have they experienced very dark, electron
dense circular deposits around the outside of the tissue? These deposits
are not within the tissue so could this be a precipitate of some sort with
some of the other chemicals used in processing? Does Imidazole react with
aldehydes/solvents to cause this type of thing?


Any comments from anyone with experience in the use of the above technique
would be appreciated!

Regards,


Richard Easingwood
South Campus Electron Microscope Unit
Otago Medical School
PO Box 913
Dunedin
NEW ZEALAND

Telephone: 64-03-479 7301
Facsimile: 64-03-479 7254

SOUTHERNMOST E.M UNIT IN THE WORLD















From: Paul.Fischione-at-internetmci.com
Date: Mon, 11 Dec 1995 20:44:50 -0500
Subject: Job Opening - Applications Engineer

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-- [ From: Paul E. Fischione * EMC.Ver #2.3 ] --

E.A. Fischione Instruments, Inc. is seeking a highly motivated individual
for an applications engineering position. Duties will be to provide both
input for new product developments and the field support of existing
products. An extensive knowledge of TEM specimen preparation techniques is
essential. The candidate must be well versed in both theoretical and
operational aspects of Transmission Electron Microscopes and must be able
to conduct routine TEM maintenance.

The ideal candidate should possess a Ph.D. in Materials Science with a B.S.
or M.S. in either engineering or physics. As an applications engineer a
great deal of activity will focus on interactions with both existing and
potential customers. Technical publications including techniques and
results developed with Fischione instrumentation will be encouraged. The
position requires excellent verbal and written communication skills and a
willingness to do extensive travel.

E.A. Fischione Instruments, Inc. was founded in 1966 and specializes in the
development and manufacture of TEM specimen preparation devices. The
company is based in Export, PA, approximately 23 miles east of the city of
Pittsburgh.

Resumes should be sent to: Paul E. Fischione
E.A. Fischione Instruments, Inc.
9003 Corporate Circle
Export, PA 15632




From: meh-at-aretha.jax.org (Margaret Hogan)
Date: Tue, 12 Dec 1995 07:42:44 -0500
Subject: glass for knives

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Message-Id: {199512121243.HAA27970-at-aretha.jax.org}
X-Sender: meh-at-aretha.jax.org
X-Mailer: Windows Eudora Version 1.4.4
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

I am looking for vendors that carry glass strips for making microtome knives.

Specifically,

4inch x 2inch x 100mm
25mm x 6.35mm x 400mm

names and phone numbers would be appreciated,

thank you!

Margaret E. Hogan, PhD, Supervisor
Biological Imaging Service
(Histology, Electron Microscopy, Image Analysis)
The Jackson Laboratory
600 Main Street
Bar Harbor, Maine 04609
(207) 288-6191
(207) 288-5079 FAX
meh-at-aretha.jax.org





From: meh-at-aretha.jax.org (Margaret Hogan)
Date: Tue, 12 Dec 1995 08:29:31 -0500
Subject: glass for knives (correction)

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Message-Id: {199512121329.IAA00651-at-aretha.jax.org}
X-Sender: meh-at-aretha.jax.org
X-Mailer: Windows Eudora Version 1.4.4
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Sorry, I just noticed my mistake on this message, the message and dimensions
should read as:

I am looking for vendors that carry glass strips for making microtome knives.

Specifically,

4inch x 2inch x 10mm
25mm x 6.35mm x 400mm

names and phone numbers would be appreciated,

thank you!

Margaret E. Hogan, PhD, Supervisor
Biological Imaging Service
(Histology, Electron Microscopy, Image Analysis)
The Jackson Laboratory
600 Main Street
Bar Harbor, Maine 04609
(207) 288-6191
(207) 288-5079 FAX
meh-at-aretha.jax.org





From: George Smith :      SMITHG-at-gar.union.edu
Date: 12 Dec 95 15:37:00 EDT
Subject: Suscribe to newsgroup

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Message-Id: {199512121910.NAA09038-at-mercury.odyssee.net}

Dear sir:
I would like to subscribe to the microscopy newsgroup. Please send any particulars.
George W. Smith
Biological Sciences
Union College
Schenectady, NY 12308
518-388-6245
518-388-6429 fax
smithg-at-gar.union.edu





From: wise-at-vaxa.cis.uwosh.edu
Date: Tue, 12 Dec 1995 16:30:30 +0000
Subject: Suscribe to newsgroup

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Thanks to all for the UMI phone numbers. Just what I needed.

Bob






From: sco.umc2-at-Mail.health.ufl.edu
Date: Tue, 12 Dec 1995 11:55:58 -0500
Subject: ROTAVIRUS IEM

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HELP. I'VE BEEN ASKED TO DO A ROTAVIRUS STUDY ON STOOL
SPECIMENS. THE PROTOCOL CALLS FOR INCUBATING THE SPECIMEN WITH
ROTAVIRUS ANTIBODY, CENTRIFUGING AT 12,000 RPM FOR 90 MINUTES,
THEN TREATING THE SPECIMEN AS A NEGATIVELY STAINED SPECIMEN.
OUR FASTEST CENTRIFUGE IS 13,000 RPM. HAVE ORDERED A POLYCOLNAL
ANTIBODY (HUMAN) TYPES 1,2,3,4,8.
QUESTIONS: CAN A SHORTER CENTRIFUGE TIME BE USED? CONCERNED
ABOUT HEAT BUILD UP, ETC.
BESIDES FLAMING ( NOT ALLOWED AT THIS HOSPITAL), WHAT
IS AN EFFECTIVE WAY TO STERILIZE THE FORCEPS/
PREVENT CARRYOVER?
BEST WAY TO FILTER/ EXTRACT VIRUS FROM STOOL
PARTICULATES?
COMMENTS FROM ANYONE WITH EXPERIENCE WITH THIS OR SIMILAR
PROCEDURES WOULD BE APPRECIATED.
BECKY GARRISON
EM LAB PH 904-549-4218
PATH DEPT/UMC FX 904-549-4290
JACKSONVILLE FL





From: MELLIOTT-at-prl.pulmonary.ubc.ca
Date: Tue, 12 Dec 1995 15:43:30 +0800PST
Subject: os/2 tracking

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Davin Jutila wrote:

We are in need of a user tracking program that will monitor the use of a
multi-user image analysis system which runs on OS/2. Does anyone
have a idea where to find such a program? Any help is appreciated.

Thank you in advance,
Davin



If you are running on Novell, you can try a program called LAN
Record.

Mark




From: Davin Jutila :      DJUTILA-at-wpsmtp.siumed.edu
Date: Tue, 12 Dec 1995 11:36:53 -0600
Subject: OS2 user tracking software

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Message-Id: {s0cd68a8.042-at-wpsmtp.siumed.edu}
X-Mailer: Novell GroupWise 4.1

Hello,

We are in need of a user tracking program that will monitor the use of a
multi-user image analysis system which runs on OS/2. Does anyone
have a idea where to find such a program? Any help is appreciated.

Thank you in advance,
Davin

Davin Jutila
Research Imaging/Flow Cytometry Facility
Southern Illinois University School of Medicine
Springfield, IL 62794-9230
E-mail: djutila-at-wpsmtp.siumed.edu





From: wise-at-VAXA.CIS.UWOSH.EDU
Date: Tue, 12 Dec 1995 12:01:58 +0000
Subject: Phone number request

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Perchance, does anyone have the phone number for Univeristy Micorfilms of
Ann Arbor MI?

Bob


Robert R. Wise, PhD
Department of Biology
University of Wisconsin Oshkosh
Oshkosh, WI 54901
(414) 424-3404 tel
(414) 424-1101 fax
wise-at-vaxa.cis.uwosh.edu






From: bergrh-at-msuvx2.memphis.edu (R. Howard Berg)
Date: Tue, 12 Dec 1995 09:18:23 +0600
Subject: Re: TEM: Artifacts(?) with Imidazole/OsO4 Fixation

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Although I haven't tested imidazole+OTO per se, I have tried a variety of
(osmium ligands+ OTO) in developing freeze substitution media, using
acetone as the solvent. Visually dark products in these samples tend to be
leached out during acetone-only rinses, so I keep the ligand in post-OTO
solutions, in which case the embedded tissue appears darker. This work is
in progress, sorry I can't offer a more complete analysis.


} Richard Lander asks:
}
} Has anyone had dealings with Imidazole + Osmium tetroxide post fixation
} methods.... And if they have, have they experienced very dark, electron
} dense circular deposits around the outside of the tissue? These deposits
} are not within the tissue so could this be a precipitate of some sort with
} some of the other chemicals used in processing? Does Imidazole react with
} aldehydes/solvents to cause this type of thing?


R. Howard Berg, Ph.D.
Biology Department
University of Memphis
Memphis, TN, 38152
E mail: bergrh-at-cc.memphis.edu
phone: 901-678-4449 fax: 901-678-4457







From: psphicas-at-pipeline.com (Phil Sphicas)
Date: Tue, 12 Dec 1995 21:45:57 -0500
Subject: TEM-Carbon coated grids

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Does anyone have a reliable way to make carbon-coated grids for high
resolution work?
I have tried floating carbon films off mica-- they seem to fall apart
before I can pick them up on grids.
Any tips or suggestions?




From: GVKM07A-at-PRODIGY.COM (DR CHARLES A GARBER)
Date: Wed, 13 Dec 1995 00:21:25 EST
Subject: glass for knives

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-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --


Margaret Hogan Wrote:
}
Subject: glass for knives (correction)

I am looking for vendors that carry glass strips for making microtome
knives. Specifically,

a) 4inch x 2inch x 10mm and b) 25mm x 6.35mm x 400mm
}
} names and phone numbers would be appreciated, thank you!

Please specify whether you are seeking to make thin sections for TEM or
thick sections for LM as the choice of glass will depend on which kind
of sections you are wanting to make.

Both types of glass are available from SPI Supplies as well as several
of the other major suppliers of EM consumables.

Chuck

======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail:
GVKM07A-at-prodigy.com
West Chester, PA 19381-0656 USA Customer Service:
SpiSupp-at-aol.com

########################################################
WWW: http://mail.cccbi.chester.pa.us/spi/spihome.html
########################################################
======================================================





From: TIETZ :      100115.66-at-CompuServe.COM
Date: 13 Dec 95 02:45:59 EST
Subject: Re: TIFF

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Hello !

The usage of slow-scan cameras is increasing very much, so there is a need to
transfer those images between different programs. Since those slow scan CCDs
produce grayscale images with more than 8 bits/pixel ( normally 12 or 14
bits/pixel ) there is a file format required that supports data up to 16
bits/pixel.

I downloaded the Aldus Tiff specification Revision 6.0, Final Q, june 3, 1992
that seems to me to be the latest one.
In this specification I found the tag 258 (102H) BitsPerSample that contains the
bits per pixel. It would be easy to write here a file with BitsPerSample=16 but
in the specification is explicitly written "Allowable values for Baseline TIFF
grayscale are 4 or 8, allowing 16 or 256 distinct shades of gray."

So what is the use of a program that could write such a file if there is no
other (different) program that is able to read this file. (See also the
discussion here about "different" TIFF formats ).

Has anyone experience how to transfer 16 bit images in a file format that is
known to a couple of image processing or graphic programs ? I am not fixed to
TIFF.

At moment I either use 8bit TIFF with previously rescaled data ( that means loss
of information) or raw format (without any header, etc.). But also the raw
format is only supported by very few programs and you have the big/liddle endian
problem.

Comments invited !

Peter Sparlinek
100115.66-at-compuserve.com





From: Dr P. Echlin :      pe13-at-cus.cam.ac.uk
Date: Wed, 13 Dec 1995 08:34:19 +0000 (GMT)
Subject: An alternative to halocarbons for CPD

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My thanks for the many messages and helpful suggestions about a=20
replacement for halocarbons. I am grateful too for the gloating=20
meterological information from Australia (25-30C is obscene at this time=20
of year).
Holiday greetings from Cambride where the snow has been replaced by gale=20
force fog.

Patrick Echlin
Multi-Imaging Centre=C5=C5




From: Nplatt :      phozak-at-molbiol.ox.ac.uk
Date: Wed, 13 Dec 1995 12:53:16 0000
Subject: unsubscribe

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Please unsubscribe my mailing address.

P. Hozak





From: ziese-at-schubert.biochem.mpg.de (Ulrike Ziese)
Date: Wed, 13 Dec 1995 14:14:59 +0100
Subject: unsubscribe

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Please subscribe

Ulrike Ziese
ziese-at-mozart.biochem.mpg.de




From: ziese-at-schubert.biochem.mpg.de (Ulrike Ziese)
Date: Wed, 13 Dec 1995 14:14:24 +0100
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Please unsubscribe

Ulrike Ziese
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From: kris-at-elod.vein.hu (Kris Kovacs)
Date: Wed, 13 Dec 1995 13:36:42 -0600
Subject: Job Opening - Machinist/Instrument Maker

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-- [ From: Paul E. Fischione * EMC.Ver #2.3 ] --

E.A. Fischione Instruments, Inc is seeking a highly skilled and motivated
individual for a senior machinist/instrument maker position. Duties will
include working with the engineering staff throughout the design and
development of innovative electron microscopy accessories including the
fabrication and intricate assembly of ultra-precise mechanical/electro-
mechanical components. Additional activity will be in the support of
production utilizing state-of-the-art CNC turning and vertical machining
centers. The candidate must be able to read engineering drawings.
Knowledge of manufacturing practices for vacuum usage is a plus.

E.A. Fischione Instruments, Inc. was founded in 1966 and specializes in the
development and manufacture of TEM specimen preparation devices. The
company is located in Export, PA, approximately 23 miles east of the city
of Pittsburgh.

Resumes should be sent to: Paul E. Fischione, President
E.A. Fischione Instruments, Inc.
9003 Corporate Circle
Export, PA 15632

Kristof KOVACS
University of Veszprem, Central Laboratory
P.O.Box 158, Veszprem, HUNGARY
H-8201
Phone: +36-(88)-421-684





From: Ian MacLaren :      MACLARIZ-at-novell2.bham.ac.uk
Date: 13 Dec 1995 13:15:36
Subject: Re: TEM-Carbon coated grids

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To: psphicas-at-pipeline.com, Microscopy-at-Sparc5.Microscopy.Com

} Does anyone have a reliable way to make carbon-coated grids for high
} resolution work?
} I have tried floating carbon films off mica-- they seem to fall apart
} before I can pick them up on grids.
} Any tips or suggestions?


Dear Phil and all other interested people,

The method we use here is as follows (as learnt from our TEM technicians):
1) Place a drop of washing up liquid or teepol on a glass slide and wipe it
all over with some tissue paper wiping most of it off in the process
(leaving the slide sightly sticky).
2) Form a thin carbon film on this slide in a carbon coater.
3) Score the carbon film with a razor blade or scalpel to suitable size
pieces.
4) Float off the pieces of carbon film with water and catch with copper
grids.
I usually prefer 200 mesh grids and it works fine every time. I use them to
support small ceramic particles in the TEM and they usually behave very well
up to at least 250k magnification.

Hope this helps

Ian MacLaren,
IRC in Materials for High Telephone: 0121 414 3447
Performance Applications, FAX: 0121 414 3441
The University of Birmingham, email: I.MacLaren-at-bham.ac.uk
Birmingham B15 2TT,
England




From: John W Heckman :      heckman-at-pilot.msu.edu
Date: Wed, 13 Dec 1995 08:23:33 -0500 (EST)
Subject: Re: TEM-Carbon coated grids

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Message-Id: {199512131323.IAA31395-at-pilot03.cl.msu.edu}

I've used two ways to fabricate carbon coated grids.

Plan A. Much as you have done. Generally it seems better to make the carbon
film on the mica surface quite thin, something around 10nm (sorry my only
monitor is mounted in a freeze-fracture machine so I can't more than speculate
on how thin). Score the resulting film into about 4mm squares, float on dH2O,
and pick them up on flamed grids. Blot at the edge to dry.

Plan B. Make conventional plastic film coated grids, by whatever means are at
your disposal, and pick them up from above on clean paper. Allow to dry then
carbon coat. Put the coated grids (film side up) into a chamber made from (e.g.)
a glass petri dish ca. 90mm in which there is a pad of filterpaper soaked with
a solvent of the film plastic. I usually let them sit over night but the plastic
is likely gone and diluted into the solvent more quickly than that. If you want
them real clean you can repeat with a fresh chamber assembly. Also makes really
nice holey carbon if you start out with glycerol doped plastic.

cheers,
John
heckman-at-pilot.msu.edu

} Does anyone have a reliable way to make carbon-coated grids for high
} resolution work?
} I have tried floating carbon films off mica-- they seem to fall apart
} before I can pick them up on grids.
} Any tips or suggestions?
}





From: kris-at-elod.vein.hu (Kris Kovacs)
Date: Wed, 13 Dec 1995 13:35:41 -0600
Subject: Job Opening - Applications Engineer

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-- [ From: Paul E. Fischione * EMC.Ver #2.3 ] --

E.A. Fischione Instruments, Inc. is seeking a highly motivated individual
for an applications engineering position. Duties will be to provide both
input for new product developments and the field support of existing
products. An extensive knowledge of TEM specimen preparation techniques is
essential. The candidate must be well versed in both theoretical and
operational aspects of Transmission Electron Microscopes and must be able
to conduct routine TEM maintenance.

The ideal candidate should possess a Ph.D. in Materials Science with a B.S.
or M.S. in either engineering or physics. As an applications engineer a
great deal of activity will focus on interactions with both existing and
potential customers. Technical publications including techniques and
results developed with Fischione instrumentation will be encouraged. The
position requires excellent verbal and written communication skills and a
willingness to do extensive travel.

E.A. Fischione Instruments, Inc. was founded in 1966 and specializes in the
development and manufacture of TEM specimen preparation devices. The
company is based in Export, PA, approximately 23 miles east of the city of
Pittsburgh.

Resumes should be sent to: Paul E. Fischione
E.A. Fischione Instruments, Inc.
9003 Corporate Circle
Export, PA 15632

Kristof KOVACS
University of Veszprem, Central Laboratory
P.O.Box 158, Veszprem, HUNGARY
H-8201
Phone: +36-(88)-421-684





From: UDZF44F-at-PRODIGY.COM (MRS KIM MURRAY)
Date: Wed, 13 Dec 1995 13:36:11 EST
Subject: Glass for knives

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-- [ From: Murray, Kim * EMC.Ver #2.10P ] --

Your request for glass for knives has been forwarded to me by the Pres.
of SPI. We can offer you the 2 sizes you have inquired about. The exact
sizes in metric are as follows:
101mm x 50.8mm x 10mm , selling price is $121.90 per box of 30
and
25.4mm x 6.4mm x 400mm, selling price is $45.00 per box of 20

Since these are not stock items for SPI, we will require a minimum
purchase of 5 boxes of each size. You need only buy one size (5 boxes)
you are not required to buy both sizes. Delivery will be about 3-4
weeks.

If you have nay questions, you may contact me by e-mail or use our toll-
free # 1-800-242-4SPI.

Kim Murray
Manager, SPI Supplies
West Chester, PA





From: INGRAM-at-RTI.ORG
Date: Wed, 13 Dec 1995 15:30:07 -0500 (EST)
Subject: Re: TEM-Carbon coated grids

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I would NOT RECOMMEND the use of Teepol or any other commercial
washing-up liquid for the preparation of carbon films for ANALYTICAL studies
using EDX or EELS - especially with biological specimens where one is interest-
ed in serious elemental quantitation!
Peter Ingram
SEND






From: sbarlow-at-sunstroke.sdsu.edu (Steve Barlow)
Date: Wed, 13 Dec 1995 09:44:28 -0800 (PST)
Subject: TP8000 baskets

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hello all

I have a user interested in obtaining baskets and wax baths for the
AO/Leica TP 8000 tissue processor
(catalog #8004 for the basket; #8005 for the wax bath).

If you have some of these items and wish to part with them , please
contact Phil Langlais directly at planglai-at-sunstroke.sdsu.edu to discuss
terms

Happy Holidays and Best Wishes for the New Year!

steve


---------------------------------------------------------------------------
------------------------------------------------------------------------------
Dr. Steve Barlow
EM Facility/Biology Dept.
San Diego State University
San Diego CA 92182-4614
phone: (619) 594-4523
fax: (619) 594-5676
email: sbarlow-at-sunstroke.sdsu.edu





From: W.L. Steffens :      STEFFENS.B-at-calc.vet.uga.edu
Date: Wed, 13 Dec 1995 08:49:37 EST
Subject: Re: ROTAVIRUS IEM

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Becky,

Our lab routinely processes human and animal stools for viral
diagnosis, and we often use IEM in one form or another. If you plan to
agglutinate the virus with specific antibody prior to applying it to the
grid, you do not need such extended centrifugation times if you are
centrifuging in 1.5 ml "microfuge" tubes. We use a speed of 12 - 13K
(about 11,000 xg) for only 5 minutes, and find this to be quite adequate.
Another approach which is worth trying is to coat the grid with
antibody first (float grid on drop of serum for about 30 min., then blot
dry), then apply the stool suspension. After incubating for 30 min.,
lightly rinse the grid to remove unbound particulates, then negative
stain. All that remains is bound, agglutinated virus. This approach is
useful for viral titer quantitation.
For sterilization, dipping forceps in a 50% clorox solution is more
than adequate (at least its acceptable to the CLIA inspectors).
We prepare stool suspensions by combining equal parts of stool and DI
water, mixing, then clarifying by centrifugation on a microfuge at 13,000
rpm (11,000 xg on our machine) for 2 minutes.



-=W.L. Steffens=-
Department of Veterinary Pathology
College of Veterinary Medicine
University of Georgia




From: Patrick Nicholson :      P.Nicholson-at-physics.gla.ac.uk
Date: Wed, 13 Dec 1995 14:52:34 GMT
Subject: European Microbeam Analysis Society - Regional Workshop

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Message-Id: {11399.199512131452-at-cepheid.physics.gla.ac.uk}

19-22 May 1996 Balatonf=FCred, Hungary

EMAS'96 2nd Regional Workshop on Modern development and applications in
Microbeam Analysis
Organised by:=20
European Microbeam Analysis society in co-operation with Hungarian
Microscopical Society Kossuth Lajos University, Debrecen

International Scientific and Organising Committee

J=E1nos L=E1b=E1r (Chairman) Hungary, Luc Van't dack Belgium, Johann=
Wernisch
Austria, Peter Karduck Germany, Patrick Nicholson U.K, Antonin Simunek Czech
Republic, Csaba Cserhati Hungary, Kristof Kovacs Hungary, Peter Nagy Hungary

WORKSHOP Secretariat
Dr. J=E1nos L=E1b=E1r
Research Institute for Technical Physics
H-1325, Budapest, PO Box 76
HUNGARY
email: labar-at-falcon.mufi.hu
* * * * * * * * * * * * * * * * * * * * * * * * *=20
* W.A. Patrick Nicholson *
* Dept. of Physics & Astronomy *
* Unversity of Glasgow *
* Glasgow G12 8QQ *
* Scotland *
* Phone: +44 141 330 4467 *=20
* Fax: +44 141 334 9029 *
* * * * * * * * * * * * * * * * * * * * * * * * *





From: S_MCGARVEY-at-SAL9K.dnet-at-gmd.fujitsu.com
Date: Wed, 13 Dec 1995 09:03:19 +0800
Subject: SEM VARS

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Hi all,

Just wanted to post my thanks for all the responses I received to
my query on Video Archiving Systems for our CD measurement SEM. It
was a huge help to hear from other folks who are currently
utilizing these types of systems.


Regards,


Steve

{ { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { {-} } } } } } } } } } } } } } } } } } } } } } } } } } } } } } }

Steve McGarvey
Photolithograhy Process Tech
Fujitsu Microelectronics, Inc.
21015 S.E. Stark Street
Gresham, Oregon, USA
97030-2099

e-mail: steve.mcgarvey-at-gmd.fujitsu.com
Fax: (503) 669-6109


"There are lies, damned lies, and statistics."

{ { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { {-} } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } }




From: waheeschen-at-dow.com (Bill Heeschen 517-636-4005 Materials/ASL)
Date: Wed, 13 Dec 1995 09:19:43 -0500
Subject: Analytical TEM Position available - The Dow Chemical Company

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Message-Id: {199512131419.AA29056-at-na3.dow.com}


POSITION OPEN

The Dow Chemical Company
Analytical Sciences
1897E Building
Midland, MI 48667

Contact: John Blackson

A R&D position in analytical transmission electron microscopy (ATEM) is
available in Dow's Surface Analysis, Microscopy and Xray Group. The
applicant should be a self motivated individual having an advanced degree
or experience in either ceramics, metallurgy or material science.
Candidate must be well versed in electron microscopy. Experience in
electron diffraction, energy dispersive spectroscopy, PEELS and sample
preparation is desirable. Other areas of competence could include: FEG-
SEM, high resolution TEM (HRTEM) and STEM. The position involves the use
of state-of-the-art equipment to solve complex industial problems. The
group is equipped with: 1 HRTEM, 1 ATEM, a dedicated STEM with EDS and
PEELS, 1 CTEM, 2 FEG-SEM's, Enviromental SEM, Analytical SEM, 2 Electron
Probes, 1 SAM, 2 XPS units, Scanning Probe Microscopies, 1 HREELS, Confocal
laser light microscope, many other light microscopes, full metallographic
capabilities, and a variety of x-ray diffractometers.

This position is also being posted on other mail reflectors and newsgroups.
12/7/95




From: Phil Piccoli :      piccoli-at-glue.umd.edu
Date: Wed, 13 Dec 1995 09:57:28 -0500 (EST)
Subject: Microscopy Position Available

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VACANCY ANNOUNCEMENT

Facility Operator

Center of Microanalysis
Department of Materials and Nuclear Engineering
A. James Clark School of Engineering
University of Maryland
College Park, MD 20742

The position of Facility Operator of the Center of Microanalysis
is vacant, and applications are invited. The person filling this
position reports to the Director of the Center. Interested
individuals are invited to apply by submitting a letter of
interest, resume and list of references (at least three) to the
address below. For optimum consideration, applications should be
received by February 1, 1996.

Duties of the position include: training and assisting users in
operating equipment and preparing samples, routine maintenance of
equipment, interfacing with service engineers to resolve major
equipment problems, working with Maryland companies to solve
microanalytical problems, and coordinating schedules and
operations.

The ideal candidate should have the following characteristics: At
least a BS degree in a field relevant to microanalysis, knowledge
of SEM, EDS, WDS, FTIR, AFM and sample preparation and skills in
personal interactions, computer operation, report writing,
coordinating schedules and ordering and maintaining materials and
supplies.


Submit applications to:

Chairman, Search Committee
Department of Materials and Nuclear Engineering
A. James Clark School of Engineering
University of Maryland
College Park, MD 20742


THE UNIVERSITY OF MARYLAND AT COLLEGE PARK IS AN EQUAL
OPPORTUNITY EMPLOYER




From: davilla-at-4pi.com (Scott D. Davilla)
Date: Wed, 13 Dec 1995 12:28:32 -0500
Subject: Re: TIFF

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} I downloaded the Aldus Tiff specification Revision 6.0, Final Q, june 3, 1992
} that seems to me to be the latest one.
} In this specification I found the tag 258 (102H) BitsPerSample that
} contains the
} bits per pixel. It would be easy to write here a file with BitsPerSample=16 but
} in the specification is explicitly written "Allowable values for Baseline TIFF
} grayscale are 4 or 8, allowing 16 or 256 distinct shades of gray."
}
} So what is the use of a program that could write such a file if there is no
} other (different) program that is able to read this file. (See also the
} discussion here about "different" TIFF formats ).
}
} Peter Sparlinek
} 100115.66-at-compuserve.com

If you read the fine print in the TIFF spec, you will find that a
TIFF reader (your program) is expected to be able to handle TIFF images
with a BitsPerSample of 4 or 8. Most good TIFF readers will also handle
16. Very good TIFF readers will handle BitsPerSample=n where n can range
from 1 to big. A good example of this is if your hardware is a 12 bit ADC.
Why waste 4 bits storing the image as a 16 bit TIFF when all you need is
12.
For our software packages, we decided that for the best
cross-program compatibility in reading our TIFF files, if our bit usage was
greater that 8, we would use 16. Not as efficent in raw storage but
cross-program compatibility was more important.
When you read the TIFF spec remember that the original TIFF was
designed for visual (look at it) images, not scientific images (crunch the
numbers). It was just flexible enough to applied to scientific images (how
about BitsPerSample=32 and the data type is 32 bit floating point -- check
the TIFF spec, a valid TIFF image but I don't think many programs could
read it).



Scott D. Davilla
4pi Analysis, Inc.
3500 Westgate Drive, Suite 403 919 489-1757 (tel)
Durham, North Carolina 27707 919 489-1487 (fax)







From: aybjg-at-flinders.edu.au (Bren Gannon)
Date: Thu, 14 Dec 1995 10:58:16 +1030
Subject: Unuscribe to newsgroup

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unsubscribe to the microscopy newsgroup.





From: LSFY69A-at-PRODIGY.COM (MR KARL H BERGER)
Date: Wed, 13 Dec 1995 18:52:47 EST
Subject: LKB Nova Cryo Parts

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If someone out there has a LKB Nova Cryo attachment or parts they
would like to part
with, please give me a holler at 908-370-8082. Thanks





From: kris-at-almos.vein.hu (Kris Kovacs)
Date: Thu, 14 Dec 1995 08:30:56 +0100
Subject: Sorry for any inconvenience...

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DEAR MICROSCOPISTS:

YOU MIGHT HAVE RECEIVED SOME JOB OPENING ANNOUNCEMENTS FROM E.A. Fischione
Instruments, Inc. OR MAYBE SOME OTHER MAIL ORIGINATED BY SOMEONE ELSE, AND
ALSO HAVING MY NAME AT THE END.

BELIEVE ME: I HAVE NOT BEEN INVOLVED IN THESE POSTINGS. HERE'S THE TRUTH:

THE SERVER I WAS CONNECTED TO IS ABOUT TO SHUT DOWN OPERATION AND IS
FORWARDING ALL MY CORRESPONDANCE TO MY NEW E-MAIL ADDRESS. WE HAVE OUR OWN
PROBLEMS WITH THIS SWITCH-OVER BUT APPARENTLY THE SYSOP OF OUR SERVER DID
AGAIN SOMETHING WRONG, AND POSTED SOME OF MY INCOMING MESSAGES AS OUTGOING
TO VARIOUS ADDRESSES, INCLUDING THIS LISTSERVER. I DON'T KNOW WHAT SHOULD I
EXPECT IN THE FUTURE, BUT PLEASE ACCEPT MY APOLOGIES FOR ANY INCONVENIENCE.

Kris


Kristof KOVACS
University of Veszprem, Central Laboratory
P.O.Box 158, Veszprem, HUNGARY
H-8201
Phone: +36-(88)-421-684





From: Ker{nen Jaakko-Tuomas :      jaakko-at-butler.cc.tut.fi
Date: Thu, 14 Dec 1995 15:46:47 +0200
Subject: unsubscrible

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unsubscrible jaakko-at-butler.cc.tut.fi




From: Kingsley-at-zephyr.nrlssc.navy.mil (KingsleyMcCrocklin)
Date: 12/14/95
Subject: Micro Plastic Grids

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-------------------------------------







From: EmLab :      EMLAB-at-OPUS.MCO.EDU
Date: Thu, 14 Dec 1995 11:03:18 -0500 (EST)
Subject: Re: ROTAVIRUS IEM

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Dear Becky,

A few questions about why you plan on doing these experiments. Do you
need only to ID rotavirus, ie. for clinical settings? or do you want to
observe the structure of the virus?
In either case, mixing the virus and antibody together will end up
with large aggragates of virus and identification will be harder. (unless you
have the proper ratio of virus:ab) The method of adding ab to a grid then
floating on top of a viral suspension works very well (this is called
immunosorbent IEM). My recommendations for a stain would be 1% ammonium
molybdate and not PTA because it has been shown that PTA can be detrimental
to viral particle structures.
Another method to consider is not an immuno procedure, one called
pseudoreplication. Here a drop of 10% viral solution (which has been
centrifuged) is placed on a square of 2-4% agar, allowed to dry, a drop of
parlodion solution is placed on agar spread around and tipped on side to drain
and dry. The agar plug (on a microscope slide) is then tilted into a dish of
stain, upon which the film is floated off, a grid is placed on top of film
and then the grid/film is picked up with the use of a small brass rod (2 cm in
length and 5mm dia) and forceps. Excess stain is drained and dried then
examined. The advantage of this procedure is that any and all viruses are
ID'ed and not just a single one.
If you need more info, please write or call.

Best of luck,
Ed Calomeni
Dept Pathology
Medical College of Ohio
Toledo, OH 43699
emlab-at-opus.mco.edu
419-381-3484




From: Hank Adams :      hadams-at-nmsu.edu
Date: Thu, 14 Dec 1995 10:33:32 -0700 (MST)
Subject: TEM: fix and embedding crustaceans

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Does anyone have any suggestions for fixation and embedding small
crustaceans (clam shrimp) about 4mm in lenght?. I'm concerned about
penetration of fixative and embedding medium through the exoskeleton; also,
the type of fixative and resin. This is for conventional LM and TEM.
I would appreciate any suggestions.
Thank you, Hank Adams
EML
New Mexico State University
Las Cruces, NM
email: hadams-at-nmsu.edu




From: AWBlackwoo-at-aol.com
Date: Thu, 14 Dec 1995 14:15:52 -0500
Subject: Oops!

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14 December 1995

Apologies are in order.

We still do not know how it happened, because the listserver is not even in
the address book of the computer from which the message was sent, but somehow
a commercial message intended for a single person made its way to the
microscopy listserver. We have to assume that somehow, somewhere we did
something wrong. As a result, we are instituting a special program to make
sure that it doesn't happen again.

Chuck Garber has asked me to send this message for him, because he has just
left on an international trip.

Again, we're sorry.

Andy

Andrew W. Blackwood, Ph.D.
Structure Probe, Inc.
63 Unquowa Road
Fairfield, CT 06430-5015
Ph: 1 203 254 0000
FAX: 1 203 254 2262
e-mail: AWBlackwoo-at-aol.com
WWW--http://mail.cccbi.chester.pa.us/spi/spihome.html




From: gbarclay-at-centre1.centre.uwi.tt (barclay - gregor fraser dr.)
Date: Thu, 14 Dec 1995 09:58:39 +0400
Subject: Light Micro Course

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The University of the West Indies in St. Augustine, Trinidad, is planning to offer a
2 week Workshop in Light Microscopy in the summer of 1996, subject to funding
approval. The course is aimed at postgraduates, technicians, and faculty from all
three campuses (in Trinidad, Jamaica, and Barbados), as well as participants from
health, government and industrial organisations from the Carribean. I am looking for
2 - 3 teachers to help in the following areas: immunocytochemistry, karyotyping, and
in situ hybridization, as well as the fundamental areas of imaging techniques and
photomicrography. As far as possible it will be a hands-on course, bearing in mind
that such equipment as a confocal microscope does not exist here. The purpose is
more to present the capabilities of the techniques rather than exhaustively perform
every method. There is tremendous need and interest in the Carribean for this kind
of workshop. The funding agency will fund travel, accomodation, etc. I invite serious
inquiries by FAX only (not e-mail, I am unsubscribing from this user group in self
defense). Dr. G. F. Barclay, Plant Science Dept., U.W.I., FAX:809-645-7132




From: gwe-at-biotech.ufl.edu (Greg Erdos)
Date: Thu, 14 Dec 1995 16:39:17 GMT
Subject: Re: TEM-Carbon coated grids

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} To: psphicas-at-pipeline.com (Phil Sphicas)
} From: gwe-at-biotech.ufl.edu (Greg Erdos)
} Subject: Re: TEM-Carbon coated grids
}
} }
} } Does anyone have a reliable way to make carbon-coated grids for high
} } resolution work?
} } I have tried floating carbon films off mica-- they seem to fall apart
} } before I can pick them up on grids.
} } Any tips or suggestions?
} }
} } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } }
}
} We routinely float carbon from mica and mount on sticky grids.
}
} after cleaving the mica, I cut it in 3-4 mm wide strips, evaporate the
carbon by a resistance method (very important),. Then I clamp the strip
carbon side up in one pair of tweezers, then I grab the other end and cut
off a square of mica. I float it off in a white porcelain spot plate and
pick it up on the grid, being careful to always blot from the side.
}
} Let me know how it goes
}
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Greg Erdos Phone: 352-392-1295
Scientific Director,
ICBR Electron Microscopy Core Lab
218 Carr Hall Fax: 352-846-0251
University of Florida E-mail: gwe-at-biotech.ufl.edu
Gainesville, FL 32611
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-





From: Daniel Possin :      oemlab-at-u.washington.edu
Date: Thu, 14 Dec 1995 15:48:44 -0800 (PST)
Subject: Re: TEM-Carbon coated grids

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Hi -

Is there a reason why you cannot evaporate carbon on to collodion coated
grids? The collodion quickly sublimes in the beam creating a very clear
window to look through. I doubt that it would be much better with a
floated film and the coating would probably be more beam stabile.

Dan

On Tue, 12 Dec 1995, Phil Sphicas wrote:

} Date: Tue, 12 Dec 1995 21:45:57 -0500
} From: Phil Sphicas {psphicas-at-pipeline.com}
} To: Microscopy-at-Sparc5.Microscopy.Com
} Subject: TEM-Carbon coated grids
}
}
} Does anyone have a reliable way to make carbon-coated grids for high
} resolution work?
} I have tried floating carbon films off mica-- they seem to fall apart
} before I can pick them up on grids.
} Any tips or suggestions?
}

%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
% %
% Daniel Possin Work: 206/ 543-7489 %
% Dept. of Ophthalmology RJ-10 FAX: 206/ 543-4414 %
% University of Washington Home: 206/ 778-1714 %
% Seattle WA 98195 USA Email: oemlab-at-u.washington.edu %
% %
% "The chinese expression 'cheung meng ba sui, gong hey fat choy' is %
% equilvalent to Vulcan expression 'live long and prosper'. It's a %
% small universe and getting smaller everyday". %
% %
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%





From: mtengows-at-students.wisc.edu (Dr. Mark Tengowski)
Date: Tue, 25 Jul 1995 17:00:37 -0500
Subject: Re: TEM: fix and embedding crustaceans

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} Does anyone have any suggestions for fixation and embedding small
} crustaceans (clam shrimp) about 4mm in lenght?. I'm concerned about
} penetration of fixative and embedding medium through the exoskeleton; also,
} the type of fixative and resin. This is for conventional LM and TEM.
} I would appreciate any suggestions.
} Thank you, Hank Adams
} EML
} New Mexico State University
} Las Cruces, NM
} email: hadams-at-nmsu.edu

Hank,

Last July I asked a similar question and go the following answers.
I have yet to try any of them so cannot add to them my experience. I'd
appreciate any additional tips you get as I have not been able to find
anything in the textbook literature.

Bob Wise

****************


Haven't had a whole lot of experience with insect tissue, but I
have done some. If you get better advice from more experienced bug people
great (but lest you get very little response).
1) Using a sharp razor blade, cut through the insect, exposing the
interior to the fixative. Doing this in fixative has seemed to work well
for the specimens I have dealt with. Cut through as close to the area of
interest as possible. (For legs we found cutting the top and bottom of the
leg was needed, I would assume the same would be true for things like
antenna, etc.). Gills are penetrated by the fixative just fine (after all
they are designed for chemical penetration).
2) Fixatives, insects tend to be hydrophobic and the addition of a
wetting agent is needed. I have used both Tween and photo-flo with
comparable results (just a few drops for say 20ml).
3) Use of a combination of 2-3% glut, 1% formaldehyde, and 1-3%
(up to 10%) acrolein (acrylic aldehyde) works well. The acrolein is a
recommended fixative for insects. I also recommended sodium cacodylate
buffer.
4) En bloc staining with UAc helps with staining, and if you're
interested specifically in walls barium permanganate post section staining,
in addition to PbCit and UAc, does unbelievable things to the walls!
5) Physically breaking the exoskeleton allows penetration of the
of the fixes, and even more lets the resins penetrate. I would use a hard
mixture of a low viscosity resin, i.e. Spurr's or quetol 651 and extend
infiltration times
*****************

Microwave-accelerated chemical fixation methods have been used with success
to fix insects for TEM:

1. Lindley VA: A new procedure for handling impervious biological
specimens. Microsc Res Tech 1992, 21:355-360

2. Smid HM, Schooneveld H, Meerloo T: Microwave fixation of
water-cooled insect tissues for immunohistochemistry. Histochem J 1990,
22:313-320

3. Contact me directly for detailed info.

Gary R. Login, D.M.D., D.M.Sc.
Assistant Professor of Oral Pathology
Beth Israel Hospital
Department of Pathology
330 Brookline Avenue
Boston, Massachusetts, 02215

glogin-at- bih.harvard.edu
Telephone: 617-667-2034
Fax: 617-667-8676
*************

Routine TEM fixation in our hands is 2.5% glutaraldehyde in 0.1M
PO4 (with 0.15M sucrose) for 1-2 hours at 4 or 20 C; wash then post fix in
1% OsO4 for 1 hr in the same buffer; wash in dist water; dehydrated in a
graded series of ethanol (acetone extracts lipids more) and infiltrate into
your resin of choice.

If you have a time consuming dissection and/or don't want to
breathe too much glut you should dissect under insect Ringers. Yes you
will have trouble getting fix thru cuticle. At very least you should
inject fix into body of larvae making sure it's in the haemolymph not the
gut lumen. If insect is big (10mm) dissection is best (if possible). If
not simply punch tiny holes thru the cuticle with needle / dissect off the
terminal half mm(if not necessary for observation / cut larvae into 2 or 3
portions and fixseparately.

If you require more exact recipes contact us directly at: erich-at-ento.csiro.au

Eric Hines, EM Unit, CSIRO Entomology, PO Box 1700, Canberra 2601 Oz
************

I did some Drosophila scanning for Sean Carroll on campus; search
for his lab's papers using SEM and confocal for possible technical ideas.

Mark W. Tengowski, DVM, MS, Postdoctoral Fellow, 309 Zoology Research, 1117
West Johnson Street, Madison, WI 53706 (608) 262-2048 lab, (608) 262-7319
fax
********






From: gente-at-gkss.de (C.Gente)
Date: Fri, 15 Dec 1995 09:53:23 +0100
Subject: texture of gold leaf

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Dear Microscopists,
I am in struggle with a possibly quiet simple and well understood problem.
Upon observations on haevily deformed fcc and bcc metal powders, e.g. Ag, Cu,
Fe and V, very typical electron-diffraction patterns were found, showing
intensity modulations on the diffraction rings. This indicates that the
materials are textured. In a book I have found a very similar diffraction
pattern to those of Ag and Cu taken from gold leaf. Unfortunately the
texture of the golg leaf is not specified in that book, but it appears to be
a fibre
texture. I would be happy if someone could answer to the following questions.
1. What is the texture of gold leaf and of the analogous treated bcc metalls,
i.e. hammered Fe.
2. Where can I find references on these topics.


Thank you very much
yours sincerely
Christof Gente





From: agoldsch-at-tamarugo.cec.uchile.cl (GOLDSCHMIDT DE LA MATTA ALFONSO)
Date: Fri, 15 Dec 1995 11:45:16 +0400
Subject: Suscribe to newsgroup

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Dear sir:
I would like to subscribe to the microscopy newsgroup. Please send any particulars.

Alfonso Goldschmidt
Elektrical Engineer
Universidad de Chile
agoldsch-at-tamarugo.cec.uchile.cl




From: agoldsch-at-tamarugo.cec.uchile.cl (GOLDSCHMIDT DE LA MATTA ALFONSO)
Date: Fri, 15 Dec 1995 11:45:16 +0400
Subject: Suscribe to newsgroup

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Dear sir:
I would like to subscribe to the microscopy newsgroup. Please send any particulars.

Alfonso Goldschmidt
Elektrical Engineer
Universidad de Chile
agoldsch-at-tamarugo.cec.uchile.cl




From: MelanieOwl-at-aol.com
Date: Fri, 15 Dec 1995 11:03:38 -0500
Subject: Re: Amray Image Archiving, etc.

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Just off the cuff, I would say that one advantage of getting the PC interface
would be that you could network the PC to your system and store images on
your LAN, so that you wouldn't need a separate storage media.
I have an even older Amray 1645 (1985 model) which is analog only, and
therefore I have to use a scanner to produce images for transfer to customers
electronically.

Let me know of the other responses to your questions, maybe something would
be useful to me as well.

Thanks,
Melanie Behrens
Senior Chemist/Microscopist
Texaco, Inc.
Beacon, NY





From: bozzola-at-siu.edu (John. J. Bozzola)
Date: Fri, 15 Dec 1995 11:58:55 -0600
Subject: SEM: replicas

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X-Sender: bozzola-at-saluki-mail.fiber2.siu.edu
Message-Id: {v01510102acf76795c5ed-at-[131.230.97.68]}
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Anyone have a good supplier for cellulose acetate sheets for making good
resolution surface replicas of hard surfaces? Thanks.

#############################################################################
John J. Bozzola, Director
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
#############################################################################






From: Raymond F Egerton :      egerton-at-phys.ualberta.ca
Date: Fri, 15 Dec 1995 09:53:26 -0700 (MST)
Subject: silicon TEM grids

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The Alberta Microelectronic Centre is contemplating marketing 3mm TEM
grids made from silicon, with each grid opening covered by a thin
electron-transparent membrane of single-crystal silicon or of amorphous
silicon dioxide or silicon nitride.

Possible applications might include direct TEM observation of thin films
deposited on the membranes, or their use as a support for biological
structures.

AMC would greatly appreciate advice from the TEM community on any of the
following questions:
(1) Would you (or someone you know) make use of such grids; if so, in what
quantity ?
(2) Are there other applications, not mentioned above ?
(3) What is the ideal membrane thickness? Are there limits on the
acceptable amount of variation in thickness? Is a grid-bar thickness of
0.4mm thickness compatible with most TEM specimen holders, or is 0.2 mm
preferable ?

Please reply to me and I will forward the information.

Thanks, Ray Egerton (egerton-at-phys.ualberta.ca)





From: psphicas-at-pipeline.com (Phil Sphicas)
Date: Fri, 15 Dec 1995 21:38:55 -0500
Subject: TEM-carbon coated grids-Thanks

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Many thanks to all the people who responded, offering tips and suggestions
for my problem.
Happy Holidays to all!


Sincerely,

Eleana Sphicas
Rockefeller University
(The Email address I am using currently is under my son's name, Phil
Sphicas).




From: baskin-at-biosci.mbp.missouri.edu (Tobias Baskin)
Date: Sun, 17 Dec 1995 11:55:15 -0600
Subject: 50W bulb lifetime summary

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Gentle networkers,
Some time ago, I asked about the lifetimes in practise for
50 W mercury arc lamps. I recieved many answers, over an extended period.
Many thanks, and here is a digest for anyone intested. The question also
came up on the confocal list, so this summary is cross posted.

Robert Josephs (bob-at-befvax.uchicago.edu) wrote:
I have a suggestion. Get a small fan (the kind used to cool electronic
circuits) to blow air through the lamp housing. This keeps the temperature
around 20-30 degrees instaed of the 200C or more. When the lamp runs this
cool it last and lasts and....
I don't know how long they last now. Before I used the fan a 100W
Hg lamp would last ca 400 hours but during the last 100 hours or so the
intensity
fluctiated a lot. Since I have been using the fan it has not been necessary
to replace the lamp. The current lamp has 500 hours and is perfectly stable.
*************

William Tivol {tivol-at-wadsworth.org} wrote:
... The biggest contribution to failure comes from thermal stress, so if
the lamp is not turned on and off, but is stabile at a particular tempera-
ture, it will last a long time.
...If you can arrange your work so that it can all get done in one
stretch, you should not be afraid to try using the lamp for 400-500 hrs,
but if you are using it in equipment which is turned on only when in use,
change the lamp when the mfr says, unless you have a surplus of housings and
a shortage of lamps.
*************

Edward J. Huff (huffe-at-pgl7.chem.nyu.edu) wrote:
We use HBO 100W/2 Zeiss lamphouse with (added) external fan, and run
for 400 to 500 hours. Lamps are Osram HBO 100W/2. I understand that
without the fan they burn out faster but I never ran them without the fan
myself.
*************

DALE A CALLAHAM {dac-at-oitunix.oit.umass.edu} wrote:
A fan is a step in the right direction for keeping things cool, but
usually isnt used since air currents on the lamp envelope can cause
excessive gradients and stress (etc....kaboom!), though people may get
away with it for the low power lamps. Also that isn't used in cases where
photometric functions are required, for stability reasons. Osram suggests
cooling of the lamp bases, the envelope needs to be hot to develop the
designed operating pressures.
****************


shaun.sandow-at-anu.edu.au (Shaun Sandow) wrote:
We run our Hg lamps (both 50 and 100W) for about 400 hours before
replacing them. After this time the 50W lamps become dim and thus need
replacing regardless of the manufacturers recommendation.
*************

R.G.White-at-sci.monash.edu.au (Rosemary White) wrote:
We've run our last two 50W HBO Hg lamps for 1) just under 300 h (a
mistake! - changed when the fluorescence got too dim) and 2) just over 200
h. As long as they don't get turned on and off too frequently they seem
OK. It
gives our Leica rep kittens, though.
***************

Benjamin Walcott {bwalcott-at-ccmail.sunysb.edu} wrote:
I find that I can run the 50 watt mercury arcs for about 170-190 hours
before I find that they either begin to flicker or the intensity of the
staining that I am using them to detect seems to drop in intensity. I have
also been told, probably by an arc lamp salesperson, that if you run them too
long beyond their normal life, they can explode. I have never had that
happen in the 10 or more years that I have been using them.
*******************

imgp-at-mbimp1.mbl.tno.nl (Kees van der Wulp) wrote:
In our setup (an older Zeiss LSM), where we only use the 50W Hg arc lamp
for focussing purposes in combination with a excitation filter that does
not affect the fluorochrome of interest, I normally use it for 300 hours
and then replace it. During the last 5 years an explosion only happened
once. The reflective mirror was broken, but the collector in the lamp house
was not damaged. You have to be careful because of the mercury vapour that
is set free. I always have a spare refletor on stock (only $ 10 and a first
componenent of the collector lens, just a few $ more).The savings by using
the lamp 300 hours are far more than the price of the spare parts.
I agree with William Tivol that switching the lamp on and off will
surely shorten the life of the lamp dramatically.
I was told by some reps that UV intensity drops remarkable after
100 hours of burning. I don't know wether that is true, but we don't use uv
excitation.
********************

DALE A CALLAHAM {dac-at-oitunix.oit.umass.edu} wrote:
The details of arc lamp operation are very simple, but also as complex as
to the differing designs of equipment used for their operation.

To start with, the ignition process always damages the lamp at each
ignition. How much damage depends on the ignitor design. The manufacturer's
life suggestion is based on 30 min of operation per start using the brute
force ignitor designs of earlier years. Some newer equipment is far more
gentle at producing a stable initial discharge and this together with
longer operation per start should be reflected in a longer life.

As for operation of the lamps, the electrode tips are eroded back to a
wider separation with ignition and operation times. This larger spacing
increases the voltage across the arc and if the lamp is operated in a
constant current mode, this means more power dissipation in the lamp and
thus more heat. Osram and others specify a maximum electrode temperature
for which the lamps are designed. The design of the lamp holder and
lamphouse will be important in determining the maximum power at which a
lamp can be safely used.

Early arc lamp equipment and some of the simpler models today (AC models
and the low priced unregulated models typically) use inductive ballasting
to effectively operate at constant current, so users with this equipment
should be cautious in exceeding recommendations.

Power supplies that operate in a constant power mode and ones that display
power readings and are regularly adjusted are probably safer to use if
playing with lamp life ratings, since the lamp temperature will be limited
to the initial value and likelyhood of explosion is reduced. In the
constant power mode, the current will be reduced as the electrode voltage
incerases and the lamp will dim until the electronics can no longer
maintain the arc (design dependent). I know that the HBO100W/2 can be
operated at 1 amp with the right power supply.

I made some measurements on a number of 100W Hg lamps (HBO 100W/2) that I
used and found that the initial arc voltage was in the range of 14.8V
(warmed up and operating at 5 amps). The arc voltage at 200 hours was as
high as 28V (-at- 5 amps) and this is 140 W!

A lamp blew the lab and trashed a quartz collector lens - price one of
those lately? I would hate to see people lose good equipment for trying to
squeeze out the last few hours of lamp life....it's not just an attempt by
the vendors to sell more lamps, although I'm sure they don't mind. The
lamps are rated safely, based on average characteristics.

Strech those dollars carefully!
*************************

"Crossman, Harold" {crossman-at-rd.sylvania.com} wrote:
My company manufactures 50W Hg microscopy lamps, and in reference
to the
recent postings I would like to offer the following suggestion. Like any
other mechanical device, pushing a lamp beyond its designed capabilities can
result in failure. I suggest changing lamps just like changing oil in a
car; when scheduled, not when things break down. We have a national
customer support center that can provide specific technical details. The
toll-free number is 1-800-LIGHTBULB.
*************************

ScottE57-at-aol.com (Scott E. Berman) wrote:
I have previously worked for Zeiss for over 10 years in microscope
sales - in that time I have found that the 100 DC HBO burners would run for
200 to 600
hours with the only problem of light output diminishing and problems then
getting even illumination over the 175 to 225 hour mark. The 50W HBO AC is a
different story - the bulb is rated at 100 hours but depending upon operating
conditions they will cloud up and darken as fast as 60-75 hours of use and I
have had in 15 years in the indusrty 4 blow up violently - they do not go
quitely like the 100 DC models. All four were in the 75 to 150 hour range.
As such I have highly encouraged people to change at 100 hours!! - the Bulbs
are same price but upon intial purchase the DC power suplly is considerably
more expensive - you pay up front and get long and safe bulb life or you save
now and pay in the number of bulbs purchased. Depending upon manufacture the
difference could be $300 for AC aupply vs. $2500 for DC supply. $2000 buys
10 to 15 bulbs and if you are a heavy fluoresence user than get the 100, if
not the 50 is a good way to put more money into the optics where it belongs.
***************************

DALE A CALLAHAM {dac-at-bio.umass.edu} wrote:
The question was about using arc lamps for more than the rated life.
The characteristics of arc lamps are the key to understanding what may
be reasonably done with them, but the wide variey of power supplies
that are available are the other part of that equation and often little
information is available to help users in this regard. Armed with the
detailed characteristics of mercury arc lamps, please go after your
manual or vendor for the missing part so that you may make the right
decisions.

Mercury arc lamps are given a nominal lifetime rating based on 30 min
of operation per start and operation at the specified conditions. Each
ignition of a mercury arc does some damage to the electrodes. A
sufficient current at a high voltage is required to break over the arc
in the cold lamp where the mercury is not in the high pressure vapor
state. This energy serves to vaporize some mercury and get the arc
established, at which point it can be maintained at a lower current and
voltage. This large surge erodes the tips of the electrodes, opening
the spacing a little each time. Both in starting and in operation, the
electrode spacing is involved in establishing the arc voltage at the
operating current. When the spacing is too large, ignition and
operation become more difficult as described below. The ignition
circuitry that Osram, for example, describes in their literature of a
few years back, is simple passive electronics that simply do the job.
More modern electronic units CAN start the lamps more gently and this,
together with longer operation per start, will be reflected in longer
usable lamp life.

Mercury arc lamps have nominal ratings: a 100W lamp operated at the
nominal 5 amps current will only give 100 watts at one brief part of
its cycle. This is because of the change of the arc electrode spacing
(and thus arc voltage). Most early power supplies, whether DC or AC
used a choke to limit and stabilize the lamp current; since the arc
voltage is much lower than the line voltage, this gave an approximation
of constant current operation. I have found that a new HBO100W/2 lamp
warmed-up and operated at 5 amps with a good DC supply has an arc
voltage of about 14.8V, giving only about 75W. At 200 hours of life,
this lamp had 28V across the arc (-at- 5 amps) and this is 140W! No doubt
this relationship would continue until a) the lamp gets too hot and
explodes, b) the power supply operating voltage becomes insufficient to
maintain 5A current (giving a decrease in power and dimming of lamp
output), or c) the power supply is unable to ignite and stabilize the
lamp at the higher arc voltage. It is possible to operate the HBO100W/2
lamp at currents as low as 1 amp with the right equipment, although the
light output is also way down. If you have a proper power supply and
the light output is adequate, operating at less than the nominal rating
should give a real extension of lamp life.

Another issue is the operating temperature of the lamp. Osram specifies
that the lamp bases should be no higher than 230C degrees and that it
SHOULD be operated at below 200C if possible. Lamp housings should be
designed to provide sufficient heat dissipation at the nominal wattage
by passive heat flow or air flow directed at the lamp bases: the quartz
envelope must be hot to have stable Hg temperature and pressure but an
excessive gradient along the lamp will produce excessive stress on the
lamp. Clearly, with economy power supplies the power dissipation
cannot be limited and at some point the lamp will get too hot and you
risk catastrophic failure. More expensive electronic supplies allow for
various sorts of automatic control or manual readout and adjustments to
control or limit power levels.

In summary, there are very good reasons that modern equipment can give
some lengthening of lamp life, but there are very real reasons not to
push this too far: the lamps simply DO get consumed in the process of
giving light. I have personally seen a 2" quartz collector shattered by
a lamp explosion and that is quite an expense to replace.
**************************************

Travis Goulette (OptoMech-at-aol.com) wrote:
Manufacturer lifetimes:

HBO 50W 100hrs.
HBO100W 200 hrs.
XBO 75W 300 hrs.

Lots of people run them much longer than the specified time. There are
potentially two big problems here:

1. After the life of the bulb is reached, the bulb starts a progressive drop
in intensity. The first wavelength affected is the UV and it creeps up from
there. Most people using FITC/Rhodamine, don't notice as much until the bulb
is affected in their wavelength (400+ hours). If you are doing quantitative
work, you will definitely want to change bulbs at the specified times.

2. Stability. The longer the bulb is run, the more the electrode and anode
are worn. As they wear, the arc becomes unstable and dances on the ends.
This causes flickering and some noise that can interfere with patch
clamping. If a bulb explodes in your lamphouse, you will find that you now
have to replace the collector lens, relay optics and sometimes the heat
shields and mirrors. The biggest cause of exploding bulbs is on/off witching
too frequently. A mercury bulb must be allowed to completely cool before be
turned back on. A xenon bulb can be refired while still hot. To prolong the
life of a bulb, it technically should be left on for long periods of time.

It should be noted that Osram has just come out with a new HBO 103 bulb which
is the new replacement for the HBO 100W and is now rated to 300 hours for
about $15-20 more than the old bulb.
***********************************

Robert Parsons {rparsons-at-INTERLOG.COM} wrote:
HBO 50 AC Arc lamps have a rated lifespan 0f 100 hours according to
Osram. (The manufacturer). This however varies according to the frequency
of starting the burner.The fewer times you start the burner the longer the
bulb life. The ratedtime of 100 hour refers to a average of two hours use
for each start.

The rule of thumb is , If the bulb is used a lot, lots of starts change it
every 100 hours. If the bulb is used for long periods, i.e. 4-8 hours at a
time, you should get 150 hours.

If the bulbs starts to flicker after 100 hours change it, if you notice
longing exposures change it.

For detailed information in the U.S. Contact Osram 1-800-431-9980 or in
Canada 416-673-1996











- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
___ ____ ^ ____ _____ Tobias I. Baskin
/ \ / / \ / \ / University of Missouri
/ | / / \ / / Biological Sciences
/___ / /__ /_____\ / /__ 109 Tucker Hall
/ / / \ ( / Columbia, MO 65211 USA
/ / / \ \ / voice: 314-882-0173
/ /____ / \ \____/ /_____ fax: 314-882-0123






From: m.dickson-at-unsw.edu.au (melvyn dickson)
Date: Mon, 18 Dec 1995 12:28:12
Subject: Re: Amray Image Archiving, etc.

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To: microscopy-at-Sparc5.Microscopy.Com

In article "Stadden, David R" {DRSTADDE+aCENTRAL%Armstrong-at-MCIMAIL.COM} writes:
} Date: Thu, 14 Dec 95 16:03 EST
} From: "Stadden, David R" {DRSTADDE+aCENTRAL%Armstrong-at-MCIMAIL.COM}
} Subject: Amray Image Archiving, etc.

. Is there something about our
} particular configuration, i.e., Amray to PC system, that requires us
} to go this comparatively expensive route to a dedicated computer with
} hard drive?

NO. You can do what we have done and install an ImageSlave. These are
inexpensive (about $3500-$5000 depending on resolution) boards which fit in a
standard PC (386, 486, whatever you have) and digitise the photo signal from
the microscope. Interfacing is very simple. We have 4. One on each STEM
Unit and one on each SEM. Since they fit in a standard PC you can send the
images out via ethernet, print them out on a laser etc. etc. Very versatile.

} Second, is there any way we could get more bang for the buck and
} archive images from our PLM with common hardware?

We have installed a CD-ROM writer. These are now $1000 or less and CD blanks
are around $10. Write two each time. A Gigabyte hard disk to store images
until you have a CD full is about $300. CD readers are a virtual standard.



} } Apart from archiving, I'd be interested in hearing
(especially from} Amray users of older scopes) about other image exploits.
Sending them} to your customers? Feasible but can be slow and
unreliable as everyone has climbed onto the net.


Printing them on laser printers?
Of course. A 1200 DPI printer makes a great job of pix. The ImageSlave
software runs in Windows so you can do instant prints off the microscope.

Photorealistic images are still pricey though.

Any
special} considerations for future compatability?

Once you have images in the PC domain as a TIFF file you can do whatever you
can imagine.}
} Thanks for whatever you may have to share,
}
} Dave Stadden
} DRStadde+aCENTRAL%ARMSTRONG-at-MCIMAIL.COM
}


I am NOT a retailer, but this system has been very satisfactory for us.

In the USA, your contact is:
Jim Hilton,
Advanced Database Systems,
7931 South Broadway #322
LITTLETON CO 80122
Tel 303-761-5635


Mel Dickson,
University of New South Wales.
Sydney Australia.




From: Andreas Brech :      andreas.brech-at-bio.uio.no
Date: Mon, 18 Dec 1995 16:14:15 +0100
Subject: Re: Amray Image Archiving, etc.

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Andreas Brech
Electron Microscopical Unit for Biological Sciences
Department of Biology, University of Oslo.
P.O.Box 1062 Blindern
N-0316 Oslo 3
Norway
Tel.: + 43-22 85 61 89 (work)
+ 43-22 43 83 23 (privat)
Fax.: + 43-22 85 47 26
e-mail.: abrech-at-bio.uio.no





From: mager-at-unixg.ubc.ca (Mary Mager)
Date: Mon, 18 Dec 1995 00:32:26 -0800
Subject: Re: Amray Image Archiving, etc.

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} Just off the cuff, I would say that one advantage of getting the PC interface
} would be that you could network the PC to your system and store images on
} your LAN, so that you wouldn't need a separate storage media.
} I have an even older Amray 1645 (1985 model) which is analog only, and
} therefore I have to use a scanner to produce images for transfer to customers
} electronically.
}
} Let me know of the other responses to your questions, maybe something would
} be useful to me as well.
}
} Thanks,
} Melanie Behrens
} Senior Chemist/Microscopist
} Texaco, Inc.
} Beacon, NY

Dear All,
Melanie makes a good point about the advantages of having a PC connected
to a network on your SEM, but where did you get the idea that only digital
SEMs can have computer imaging on them? There are many PC systems for
taking images off your SEM and storing them as digital images on the PC
computer and as far as I know they all work fine on any SEM. There are both
active systems, which have their own scan generators (beam steer) and
passive systems which use the wave forms generated by your SEM's own scan
generator to form the image on the computer (passive capture). Some of the
systems I know of are: Quartz PCI, Image Slave (Australia), Printerface
from GW, Semicaps and I'm sure there are others I have neglected. They will
all interface to old, analog SEMs. I know of a Quartz PCI system running on
a 1970 Nanolab and a Cambridge 250 (1980).
The price of big hard drives is quite low now and the Iomega Zip drive,
which stores 100 MBytes on each removeable disk is a reasonable price
(~$225US). 1K by 1K by 8 bit images are less than 1MByte each in TIFF
format, so you can get a lot on a gigabyte drive. Even burning your own
CD-ROMs for image archiving has dropped to an almost reasonable price
($900US).
Best of luck and have fun. You'll love your computer imaging system.
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Eng, UBC
309 - 6350 Stores Rd.
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648, fax: 604-822-3619
email: mager-at-unixg.ubc.ca






From: NANCY SMITH :      NSMITH-at-darwin.sci.csuhayward.edu
Date: Mon, 18 Dec 1995 11:05:44 PSD8PDT
Subject: unsubscribe

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Unsubscribe Nancy R. Smith nsmith-at-csuhayward.edu




From: Sergio C Feijoo :      scf1-at-Ra.MsState.Edu
Date: Mon, 18 Dec 1995 13:39:28 -0600 (CST)
Subject: SEM preparation for spores in ice cream mix

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We have directed this question to several researchers in the US
and Europe, to the Usenet and now - as a last resort - to this
microscopy list. Hopefully, I won't need to keep my fingers
crossed for too long :)

We are trying to use SEM to study possible changes in morphology
of spores of Bacillus licheniformes added to ice cream mix that
contains 15% fat (which in turn undergoes a special treatment).

The first fixation protococol (glutaraldehyde & osmium tetroxide,
centrifuging at all steps, etc) resulted in only being able to see
the processed mix fat and no visible spore. It seems that a fine
coat of lipid material (???) covers the "lumps", which we assume
should be the spores. Acetone and chloroform was also used, but
without any success.

We have/had no problem to visualize the spores when added to phosphate
buffer; the SEM protocol used did work fine. However, it seems that
the high fat content and the "fluid" nature of the mix are the real
problem for us. Cheese, which is a solid matrix of fat + proteins,
does not pose any problem. We can remove all the fat (leaving holes)
and nicely see the bacterias present as well as the protein matrix
(casein) that constitute cheeses in general.

Can anyone suggest a method of removing the fat and leaving the spores
"uncoated" so they can be visualized during SEM preparation ?

Thanks for your comments and Merry Christmas to all.



Sergio Feijoo
scf1-at-ra.msstate.edu




From: bhirche-at-usit.net (Bob)
Date: Mon, 18 Dec 1995 15:50:59 -0500
Subject: Image archive system - Windows 95 problem

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Message-Id: {199512181357.HAA17151-at-Sparc5.Microscopy.Com}
"Neuberger,Damian" {neuberd-at-engrnd.roundlake.baxter.com}







From: cook-at-aaem.amc.anl.gov (Russell E. Cook)
Date: Mon, 18 Dec 1995 14:32:08 -0600
Subject: Aquiring digitized SEM images

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Mary Mager mentioned PC-based systems (Quartz PCI, Image Slave, Printerface
from GW, Semicaps). We have a Mac-based system to acuire digital SEM
images from an older SEM. The interface is produced by 4pi, Inc.

Russell E. Cook
Electron Microscopy Center for Materials Research
Argonne National Laboratory
9700 South Cass Avenue
MSD-212
Argonne, IL 60439
(708)252-7194
FAX: (708)252-4798






From: Hank Adams :      hadams-at-nmsu.edu
Date: Mon, 18 Dec 1995 14:31:02 -0700 (MST)
Subject: TEM:water miscible embedding media

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I need to embed plant material (leaf) for immunocytochemistry. The
problem is that the protein we're looking for is believed to be embedded in
the waxy cuticle. We're afraid of losing the protein with conventional
dehydration and embedding protocols. I have no idea of the solubilities
of the waxes which are esters if fatty acids and alcohols having 26 - 34
carbon atoms. Does anyone have any experience with this type of material
and the use of water miscible resins. I know there are several different
resins that are water miscible & commercially available, but are they
applicable in this case and useful for immunocytochemistry?
Thank you,
Hank Adams
EML, New Mexico State Univ.






From: psphicas-at-pipeline.com (Phil Sphicas)
Date: Mon, 18 Dec 1995 18:57:50 -0500
Subject: Subject: TEM-Bacteriophage IEM

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Does anyone know of a good, reliable way to produce images of negatively
stained specimens after immunogold labeling? I have tried the following
two methods with bacterophages.

Method A. The protocol followed was:
(1) Adsorb properly diluted phage sample onto glow-discharge plastic-carbon
coated grid for one minute.
(2) Incubate with 1st antibody for 30 minutes.
(3) Rinse the grids with buffer, or pass the grids over drops of buffer.
(4) Incubate with 2nd antibody-gold for 30 minutes.
(5) Rinse again.
(6) Negative stain.
The difficulty with this method I have encountered is that after the
procedure is applied either the film breaks as soon as the electron beam
hits, or most of the phages are lost. (Without IEM, the negative-stained
phages were fine).

Method B: Incubate the phages with 1st antibody and 2nd antibody-gold in a
microfuge tube and then negative stain. This method works well, but the
specimen appears to have a very high background of gold particles.

I wonder if others have similar difficulties and how they are dealing with
them. Any information or suggestions would be appreciated.

Eleana Sphicas
Rockefeller University




From: shirley.turner-at-nist.gov (Shirley Turner)
Date: Mon, 18 Dec 1995 21:26:56 -0400 (EDT)
Subject: Postdoctoral positions at NIST

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POSTDOCTORAL POSITIONS AT NIST


The National Institute of Standards and Technology (NIST) is interested in
receiving applicants for National Research Council (NRC) postdoctoral
positions. In particular, applicants with interests in electron microscopy
are welcomed for work in the Chemical Science and Technology Laboratory.
Projects can be proposed in the fields of material science, chemistry,
mineralogy or other related fields using a variety of techniques including
high-resolution TEM, EDS, PEELS, holography, and electron energy loss
imaging, etc.


INSTRUMENTATION AVAILABLE: A Philips CM300 FEG equipped with a Gatan
Imaging Filter and a Philips CM30 with a PEELS unit are the primary TEM
instruments. There are a variety of associated instruments available
including scanning electron microscopes, secondary ion microprobes, x-ray
diffraction units, etc.

SALARY: $45,500 per year (2 year appointments)

APPLICATION (WITH PROPOSAL) DUE BY: January 16, 1996

EXPECTED STARTING DATE: Fall 1986 (PhD must be complete by this time)

TO OBTAIN APPLICATION: contact Eric Steel (301) 975-3902, eric.steel-at-nist.gov.


NOTE: US Citizenship is required.





From: mager-at-unixg.ubc.ca (Mary Mager)
Date: Mon, 18 Dec 1995 22:45:10 -0800
Subject: Re: Humor

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Message-Id: {199512181503.JAA17235-at-Sparc5.Microscopy.Com}

Dear Ron,
Thank you from all of us in Microscopy Listserver. Your humour livened up
an otherwise typical Monday.
Thanks,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Eng, UBC
309 - 6350 Stores Rd.
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648, fax: 604-822-3619
email: mager-at-unixg.ubc.ca






From: gwe-at-biotech.ufl.edu (Greg Erdos)
Date: Tue, 19 Dec 1995 09:23:45 GMT
Subject: Re: Subject: TEM-Bacteriophage IEM

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}
} Does anyone know of a good, reliable way to produce images of negatively
} stained specimens after immunogold labeling? I have tried the following
} two methods with bacterophages.
}
} Method A. The protocol followed was:
} (1) Adsorb properly diluted phage sample onto glow-discharge plastic-carbon
} coated grid for one minute.
} (2) Incubate with 1st antibody for 30 minutes.
} (3) Rinse the grids with buffer, or pass the grids over drops of buffer.
} (4) Incubate with 2nd antibody-gold for 30 minutes.
} (5) Rinse again.
} (6) Negative stain.
} The difficulty with this method I have encountered is that after the
} procedure is applied either the film breaks as soon as the electron beam
} hits, or most of the phages are lost. (Without IEM, the negative-stained
} phages were fine).
}
} Method B: Incubate the phages with 1st antibody and 2nd antibody-gold in a
} microfuge tube and then negative stain. This method works well, but the
} specimen appears to have a very high background of gold particles.
}
} I wonder if others have similar difficulties and how they are dealing with
} them. Any information or suggestions would be appreciated.
}
} Eleana Sphicas
} Rockefeller University
}
} } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } }

In general I have always had success with your method #1. I would suggest a
Blocking step after applying the phage and before applying the primary antibody.

Also use as small a mesh size for your grid as possible and use nickel not
copper. I have always done it with 400 mesh grids, but smaller are
available if you a re have trouble with the film breaking. Other wise just
try to minimize mechanical damage as much as possible.

Good luck
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Greg Erdos Phone: 352-392-1295
Scientific Director,
ICBR Electron Microscopy Core Lab
218 Carr Hall Fax: 352-846-0251
University of Florida E-mail: gwe-at-biotech.ufl.edu
Gainesville, FL 32611
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-





From: gwe-at-biotech.ufl.edu (Greg Erdos)
Date: Tue, 19 Dec 1995 09:11:06 GMT
Subject: Re: SEM preparation for spores in ice cream mix

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}
}
}
} We have directed this question to several researchers in the US
} and Europe, to the Usenet and now - as a last resort - to this
} microscopy list. Hopefully, I won't need to keep my fingers
} crossed for too long :)
}
} We are trying to use SEM to study possible changes in morphology
} of spores of Bacillus licheniformes added to ice cream mix that
} contains 15% fat (which in turn undergoes a special treatment).
}
} The first fixation protococol (glutaraldehyde & osmium tetroxide,
} centrifuging at all steps, etc) resulted in only being able to see
} the processed mix fat and no visible spore. It seems that a fine
} coat of lipid material (???) covers the "lumps", which we assume
} should be the spores. Acetone and chloroform was also used, but
} without any success.
}
} We have/had no problem to visualize the spores when added to phosphate
} buffer; the SEM protocol used did work fine. However, it seems that
} the high fat content and the "fluid" nature of the mix are the real
} problem for us. Cheese, which is a solid matrix of fat + proteins,
} does not pose any problem. We can remove all the fat (leaving holes)
} and nicely see the bacterias present as well as the protein matrix
} (casein) that constitute cheeses in general.
}
} Can anyone suggest a method of removing the fat and leaving the spores
} "uncoated" so they can be visualized during SEM preparation ?
}
} Thanks for your comments and Merry Christmas to all.
}
}
}
} Sergio Feijoo
} scf1-at-ra.msstate.edu
}
} } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } }

The best thing you might do is to use cryo-SEM and fracture the frozen
specimen, sublime away some of the ice, coat it and observe it in the frozen
hdrated condition. This requires special aparattus. If you have a
freeze-fracture device available it might be possible to do that and observe
the C-Pt replicas either by SEM or better by TEM. If you don't have one,
there is one at the Univ. of Georgia.

It might also be possible to dry fracture your sample in some way
just before you coat it. I don't know what kind of a chunk you end up with
or is it a powder. If it is a power, try putting it between two pieces of
scoth tape and then rip it apart, hoping to expose your spores. If it is a
piece, tease it apart with needles ar scalpels.

I have found high lipid stuff very difficult to deal with by
conventional SEM
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Greg Erdos Phone: 352-392-1295
Scientific Director,
ICBR Electron Microscopy Core Lab
218 Carr Hall Fax: 352-846-0251
University of Florida E-mail: gwe-at-biotech.ufl.edu
Gainesville, FL 32611
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-





From: bozzola-at-siu.edu (John. J. Bozzola)
Date: Tue, 19 Dec 1995 15:23:59 -0600
Subject: SEM: administration

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A colleague has asked for information. They are establishing a centrally
administered SEM unit which will be available for researchers at his
university. There will be a chargeback system and there will also be
internal money or time available for instrument useage. This would be on a
competitive basis (i.e., internal competitive money). Does anyone have in
place a document or application that would detail procedures for applying
for such internal research money or for scope time? Please direct your
responses to Dr. Michael Dingerson, Assoc. Vice Chancellor for Research at
mdingers-at-sunset.backbone.olemiss.edu
OR you may phone him at: 601-232-7482. Many thanks and happy holidays to all!
John


#############################################################################
John J. Bozzola, Director
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
#############################################################################






From: John G Humenansky :      humen001-at-maroon.tc.umn.edu
Date: Tue, 19 Dec 1995 23:46:48 -0600 (CST)
Subject: unsubscribe

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unsubscribe humen001-at-maroon.tc.umn.edu




From: azriel gorski :      azrielg-at-cc.huji.ac.il
Date: Wed, 20 Dec 1995 10:33:55 +0200 (IST)
Subject: Re: Framable Micrographs

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On Tue, 19 Dec 1995, Fiona Leek wrote:

} MHS: Source date is: 19-Dec-95 07:55 EDT
}
} I am looking for a source of polarized light micrographs
} (preferably of colourful materials ie LC, spherulites
} etc.) that have been enlarged and are suitable for framing.
}
} Any suggestions?
}

Try pp-DDT coming out of a melt. It give beautiful and colorful flowers.
I use it regularly to give a "gift of flowers" to tours, watching them
form on the video screen.

I hope this helps.

Shalom from Jerusalem,
Azriel Gorski

++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
Azriel Gorski, PhD
Head, Optical Microscopy Laboratory
Division of Identification and Forensic Science
Israel National Police
Jerusalem, ISRAEL




From: OptoMech-at-aol.com
Date: Wed, 20 Dec 1995 11:03:13 -0500
Subject: Re: Framable Micrographs

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Try Nikon's 1996 'small world' microscope calendar. It is full of microscope
images, some of which are taken with polarized light!

M. Coombs





From: roberts-at-CSSS.la.asu.edu (Bob Roberts)
Date: Wed, 20 Dec 1995 09:12:45 -0700
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
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Message-Id: {v01530500acfde62f529e-at-[129.219.51.66]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

unsubscribe roberts-at-csss.la.asu.edu






From: RNBALDUC-at-ARCRIDE.EDU.AR
Date: Wed, 20 Dec 1995 12:21 -0300
Subject: searching SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


\We are searching an used SEM ( working, if it is possible )for received
it in donation .
Please, contact me for any questions.

E-mail : RNBALDUC-at-arcide.edu.ar

Fernando Balducci
Laboratory of Electron Microscopy
School of Bioengineering.
National University of Entre Rios.
C.C 57 Suc 3
Parana - Entre Rios
Argentina.





From: VayTek, Inc. :      vaytek-at-netins.net
Date: Wed, 20 Dec 1995 11:45:08 -0600
Subject: Subscribe

Contents Retrieved from Microscopy Listserver Archives
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Vaytek needs to resubscribe to Microscopy list. Our current internet
provider has changed the domain name from ins.infonet.net to netins.net.

Vaytek's new email address is: Vaytek-at-netins.net

Mail sent to ins.infonet.net will not reach us. Sorry for any inconvenience
this has caused.

Regards
David Fleshman
Vaytek





From: gwe-at-biotech.ufl.edu (Greg Erdos)
Date: Wed, 20 Dec 1995 13:49:02 GMT
Subject: carbon films followup

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I have had several questions about what I mean when I refer to resistance
evaporation. If current passes directly through the carbon source and thus
heating it, this is resistance evaporation. So if one is using carbon
thread or a pair of carbon rods that touch, you are using electrical
resistance to generate the heat.

The alternate method is electron beam evaporation where the carbon source is
positioned in the center of a tungsten coil, but not touching it. When
current is applied to the tungsten under vacuum, electrons are produced
which then bombard the carbon and thus evaporate it.

Sorry for the confusion.
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Greg Erdos Phone: 352-392-1295
Scientific Director,
ICBR Electron Microscopy Core Lab
218 Carr Hall Fax: 352-846-0251
University of Florida E-mail: gwe-at-biotech.ufl.edu
Gainesville, FL 32611
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-





From: Daniel Possin :      oemlab-at-u.washington.edu
Date: Wed, 20 Dec 1995 09:36:39 -0800 (PST)
Subject: LR White puckers?

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X-Sender: oemlab-at-saul2.u.washington.edu

Hello everyone-

We have been using LR White embedding medium for LM and EM
immunocytochemistry in our lab for several years now and lately we have
been having a problem we have been unable to solve. LR White has always
been more difficult to mount to slides and/or grids than other mediums,
but the problem has seemed to become worse. It has become particularly
annoying to find that it has developed many small puckers or bubbles in
sections mounted on formvar coated grids after processing for immunogold
labeling. We believe that there may have been a change in the
formulation of the product because sections from blocks embedded over a
year ago do not seem to have this problem and our embedding procedure
hasn't changed. Are any of you having this same problem? Have any of you
solved this problem? If you don't have this problem, please tell me what
you are doing so we can compare notes. Thanks in advance.


% Daniel Possin Work: 206/ 543-7489 %
% Dept. of Ophthalmology RJ-10 FAX: 206/ 543-4414 %
% University of Washington Home: 206/ 778-1714 %
% Seattle WA 98195 USA Email: oemlab-at-u.washington.edu %
% %
% "The chinese expression 'cheung meng ba sui, gong hey fat choy' is %
% equilvalent to Vulcan expression 'live long and prosper'. It's a %
% small universe and getting smaller everyday". %





From: EvexAnalyt-at-aol.com
Date: Wed, 20 Dec 1995 12:42:36 -0500
Subject: Re: SEM VARS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Evex Analytical Instruments


Thank you for expressing interest in the VIDX Scan Active Digital Imaging
System, the Fast, High Resolution SEM Image Acquisition.

Features VIDX Scan Active Digital Imaging System,
- Menu Driven
- Quick button operation
- X-ray mapping in minutes
- Multisouces acquisition
- 12 Bit high resolution image acquisition 4096 gray Levels
- Independent contrast for each source
- User defined resolution
- Pixel-by-pixel, multi-signal digitization

Now capture up to 6 high resolution images from SEM, STEM, EDS, WDS. Images
are scanned from the SEM, directly into the PC as an image file. All images
are stored in standard TIFF format. Archive your images on your Hard Drive,
Tape Backup, CD Writer. Process your images using Image Pro, the world's
greatest image processing software. Write Reports - Import images
effortlessly into your word processor. Print to the 1200x600 Laser Printer
giving Photo realistic quality. Network your system via the PC.

You will be able to capture images quickly and economically without the use
of expensive and time consuming micrographs.

The VIDX Scan Active Digital Imaging System, actively controls the SEM Scan,
thus allowing multi-frame image integration. Multiple time constants allow
the scan to be slowed for high noise situation such as low voltage or low
spot size conditions. Images can be stored with SEM data (micron bars, user
comments) permanently overlaid on the image, or stored in the image file
without overwriting any part of the image.

The pixel-by-pixel, multi-signal digitization method will assure the
precision necessary to overlay images acquired from different sources.

If you would like to learn more about VIDX Technology products contact your
Service & Sales representative at (908)874-3800. We look forward to
satisfying your X-ray Microanalysis & Imaging needs.







From: EvexAnalyt-at-aol.com
Date: Wed, 20 Dec 1995 12:42:52 -0500
Subject: Re: TEM/SEM courses

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Evex Analytical Instruments


Thank you for expressing interest in the VIDX Scan Active Digital Imaging
System, the Fast, High Resolution SEM Image Acquisition.

Features VIDX Scan Active Digital Imaging System,
- Menu Driven
- Quick button operation
- X-ray mapping in minutes
- Multisouces acquisition
- 12 Bit high resolution image acquisition 4096 gray Levels
- Independent contrast for each source
- User defined resolution
- Pixel-by-pixel, multi-signal digitization

Now capture up to 6 high resolution images from SEM, STEM, EDS, WDS. Images
are scanned from the SEM, directly into the PC as an image file. All images
are stored in standard TIFF format. Archive your images on your Hard Drive,
Tape Backup, CD Writer. Process your images using Image Pro, the world's
greatest image processing software. Write Reports - Import images
effortlessly into your word processor. Print to the 1200x600 Laser Printer
giving Photo realistic quality. Network your system via the PC.

You will be able to capture images quickly and economically without the use
of expensive and time consuming micrographs.

The VIDX Scan Active Digital Imaging System, actively controls the SEM Scan,
thus allowing multi-frame image integration. Multiple time constants allow
the scan to be slowed for high noise situation such as low voltage or low
spot size conditions. Images can be stored with SEM data (micron bars, user
comments) permanently overlaid on the image, or stored in the image file
without overwriting any part of the image.

The pixel-by-pixel, multi-signal digitization method will assure the
precision necessary to overlay images acquired from different sources.

If you would like to learn more about VIDX Technology products contact your
Service & Sales representative at (908)874-3800. We look forward to
satisfying your X-ray Microanalysis & Imaging needs.







From: EvexAnalyt-at-aol.com
Date: Wed, 20 Dec 1995 12:42:07 -0500
Subject: Re: Aquiring digitized SEM Images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Evex Analytical Instruments


Thank you for expressing interest in the VIDX Scan Active Digital Imaging
System, the Fast, High Resolution SEM Image Acquisition.

Features VIDX Scan Active Digital Imaging System,
- Menu Driven
- Quick button operation
- X-ray mapping in minutes
- Multisouces acquisition
- 12 Bit high resolution image acquisition 4096 gray Levels
- Independent contrast for each source
- User defined resolution
- Pixel-by-pixel, multi-signal digitization

Now capture up to 6 high resolution images from SEM, STEM, EDS, WDS. Images
are scanned from the SEM, directly into the PC as an image file. All images
are stored in standard TIFF format. Archive your images on your Hard Drive,
Tape Backup, CD Writer. Process your images using Image Pro, the world's
greatest image processing software. Write Reports - Import images
effortlessly into your word processor. Print to the 1200x600 Laser Printer
giving Photo realistic quality. Network your system via the PC.

You will be able to capture images quickly and economically without the use
of expensive and time consuming micrographs.

The VIDX Scan Active Digital Imaging System, actively controls the SEM Scan,
thus allowing multi-frame image integration. Multiple time constants allow
the scan to be slowed for high noise situation such as low voltage or low
spot size conditions. Images can be stored with SEM data (micron bars, user
comments) permanently overlaid on the image, or stored in the image file
without overwriting any part of the image.

The pixel-by-pixel, multi-signal digitization method will assure the
precision necessary to overlay images acquired from different sources.

If you would like to learn more about VIDX Technology products contact your
Service & Sales representative at (908)874-3800. We look forward to
satisfying your X-ray Microanalysis & Imaging needs.







From: EvexAnalyt-at-aol.com
Date: Wed, 20 Dec 1995 12:42:15 -0500
Subject: Re: Amray Image Archiving, etc.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Evex Analytical Instruments


Thank you for expressing interest in the VIDX Scan Active Digital Imaging
System, the Fast, High Resolution SEM Image Acquisition.

Features VIDX Scan Active Digital Imaging System,
- Menu Driven
- Quick button operation
- X-ray mapping in minutes
- Multisouces acquisition
- 12 Bit high resolution image acquisition 4096 gray Levels
- Independent contrast for each source
- User defined resolution
- Pixel-by-pixel, multi-signal digitization

Now capture up to 6 high resolution images from SEM, STEM, EDS, WDS. Images
are scanned from the SEM, directly into the PC as an image file. All images
are stored in standard TIFF format. Archive your images on your Hard Drive,
Tape Backup, CD Writer. Process your images using Image Pro, the world's
greatest image processing software. Write Reports - Import images
effortlessly into your word processor. Print to the 1200x600 Laser Printer
giving Photo realistic quality. Network your system via the PC.

You will be able to capture images quickly and economically without the use
of expensive and time consuming micrographs.

The VIDX Scan Active Digital Imaging System, actively controls the SEM Scan,
thus allowing multi-frame image integration. Multiple time constants allow
the scan to be slowed for high noise situation such as low voltage or low
spot size conditions. Images can be stored with SEM data (micron bars, user
comments) permanently overlaid on the image, or stored in the image file
without overwriting any part of the image.

The pixel-by-pixel, multi-signal digitization method will assure the
precision necessary to overlay images acquired from different sources.

If you would like to learn more about VIDX Technology products contact your
Service & Sales representative at (908)874-3800. We look forward to
satisfying your X-ray Microanalysis & Imaging needs.







From: EvexAnalyt-at-aol.com
Date: Wed, 20 Dec 1995 12:42:21 -0500
Subject: Re: Amray Image Archiving, etc.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Evex Analytical Instruments


Thank you for expressing interest in the VIDX Scan Active Digital Imaging
System, the Fast, High Resolution SEM Image Acquisition.

Features VIDX Scan Active Digital Imaging System,
- Menu Driven
- Quick button operation
- X-ray mapping in minutes
- Multisouces acquisition
- 12 Bit high resolution image acquisition 4096 gray Levels
- Independent contrast for each source
- User defined resolution
- Pixel-by-pixel, multi-signal digitization

Now capture up to 6 high resolution images from SEM, STEM, EDS, WDS. Images
are scanned from the SEM, directly into the PC as an image file. All images
are stored in standard TIFF format. Archive your images on your Hard Drive,
Tape Backup, CD Writer. Process your images using Image Pro, the world's
greatest image processing software. Write Reports - Import images
effortlessly into your word processor. Print to the 1200x600 Laser Printer
giving Photo realistic quality. Network your system via the PC.

You will be able to capture images quickly and economically without the use
of expensive and time consuming micrographs.

The VIDX Scan Active Digital Imaging System, actively controls the SEM Scan,
thus allowing multi-frame image integration. Multiple time constants allow
the scan to be slowed for high noise situation such as low voltage or low
spot size conditions. Images can be stored with SEM data (micron bars, user
comments) permanently overlaid on the image, or stored in the image file
without overwriting any part of the image.

The pixel-by-pixel, multi-signal digitization method will assure the
precision necessary to overlay images acquired from different sources.

If you would like to learn more about VIDX Technology products contact your
Service & Sales representative at (908)874-3800. We look forward to
satisfying your X-ray Microanalysis & Imaging needs.







From: EvexAnalyt-at-aol.com
Date: Wed, 20 Dec 1995 12:42:48 -0500
Subject: Evex Analytical Instruments

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Evex Analytical Instruments


Thank you for expressing interest in the VIDX Scan Active Digital Imaging
System, the Fast, High Resolution SEM Image Acquisition.

Features VIDX Scan Active Digital Imaging System,
- Menu Driven
- Quick button operation
- X-ray mapping in minutes
- Multisouces acquisition
- 12 Bit high resolution image acquisition 4096 gray Levels
- Independent contrast for each source
- User defined resolution
- Pixel-by-pixel, multi-signal digitization

Now capture up to 6 high resolution images from SEM, STEM, EDS, WDS. Images
are scanned from the SEM, directly into the PC as an image file. All images
are stored in standard TIFF format. Archive your images on your Hard Drive,
Tape Backup, CD Writer. Process your images using Image Pro, the world's
greatest image processing software. Write Reports - Import images
effortlessly into your word processor. Print to the 1200x600 Laser Printer
giving Photo realistic quality. Network your system via the PC.

You will be able to capture images quickly and economically without the use
of expensive and time consuming micrographs.

The VIDX Scan Active Digital Imaging System, actively controls the SEM Scan,
thus allowing multi-frame image integration. Multiple time constants allow
the scan to be slowed for high noise situation such as low voltage or low
spot size conditions. Images can be stored with SEM data (micron bars, user
comments) permanently overlaid on the image, or stored in the image file
without overwriting any part of the image.

The pixel-by-pixel, multi-signal digitization method will assure the
precision necessary to overlay images acquired from different sources.

If you would like to learn more about VIDX Technology products contact your
Service & Sales representative at (908)874-3800. We look forward to
satisfying your X-ray Microanalysis & Imaging needs.







From: EvexAnalyt-at-aol.com
Date: Wed, 20 Dec 1995 12:42:11 -0500
Subject: Re: Aquiring digitized SEM images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Evex Analytical Instruments


Thank you for expressing interest in the VIDX Scan Active Digital Imaging
System, the Fast, High Resolution SEM Image Acquisition.

Features VIDX Scan Active Digital Imaging System,
- Menu Driven
- Quick button operation
- X-ray mapping in minutes
- Multisouces acquisition
- 12 Bit high resolution image acquisition 4096 gray Levels
- Independent contrast for each source
- User defined resolution
- Pixel-by-pixel, multi-signal digitization

Now capture up to 6 high resolution images from SEM, STEM, EDS, WDS. Images
are scanned from the SEM, directly into the PC as an image file. All images
are stored in standard TIFF format. Archive your images on your Hard Drive,
Tape Backup, CD Writer. Process your images using Image Pro, the world's
greatest image processing software. Write Reports - Import images
effortlessly into your word processor. Print to the 1200x600 Laser Printer
giving Photo realistic quality. Network your system via the PC.

You will be able to capture images quickly and economically without the use
of expensive and time consuming micrographs.

The VIDX Scan Active Digital Imaging System, actively controls the SEM Scan,
thus allowing multi-frame image integration. Multiple time constants allow
the scan to be slowed for high noise situation such as low voltage or low
spot size conditions. Images can be stored with SEM data (micron bars, user
comments) permanently overlaid on the image, or stored in the image file
without overwriting any part of the image.

The pixel-by-pixel, multi-signal digitization method will assure the
precision necessary to overlay images acquired from different sources.

If you would like to learn more about VIDX Technology products contact your
Service & Sales representative at (908)874-3800. We look forward to
satisfying your X-ray Microanalysis & Imaging needs.







From: EvexAnalyt-at-aol.com
Date: Wed, 20 Dec 1995 12:42:31 -0500
Subject: Re: SEM Job Opening

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

microscopy-at-Sparc5.Microscopy.Com

Evex Analytical Instruments


Thank you for expressing interest in the VIDX Scan Active Digital Imaging
System, the Fast, High Resolution SEM Image Acquisition.

Features VIDX Scan Active Digital Imaging System,
- Menu Driven
- Quick button operation
- X-ray mapping in minutes
- Multisouces acquisition
- 12 Bit high resolution image acquisition 4096 gray Levels
- Independent contrast for each source
- User defined resolution
- Pixel-by-pixel, multi-signal digitization

Now capture up to 6 high resolution images from SEM, STEM, EDS, WDS. Images
are scanned from the SEM, directly into the PC as an image file. All images
are stored in standard TIFF format. Archive your images on your Hard Drive,
Tape Backup, CD Writer. Process your images using Image Pro, the world's
greatest image processing software. Write Reports - Import images
effortlessly into your word processor. Print to the 1200x600 Laser Printer
giving Photo realistic quality. Network your system via the PC.

You will be able to capture images quickly and economically without the use
of expensive and time consuming micrographs.

The VIDX Scan Active Digital Imaging System, actively controls the SEM Scan,
thus allowing multi-frame image integration. Multiple time constants allow
the scan to be slowed for high noise situation such as low voltage or low
spot size conditions. Images can be stored with SEM data (micron bars, user
comments) permanently overlaid on the image, or stored in the image file
without overwriting any part of the image.

The pixel-by-pixel, multi-signal digitization method will assure the
precision necessary to overlay images acquired from different sources.

If you would like to learn more about VIDX Technology products contact your
Service & Sales representative at (908)874-3800. We look forward to
satisfying your X-ray Microanalysis & Imaging needs.







From: zaluzec-at-Microscopy.Com (Nestor J. Zaluzec)
Date: Wed, 20 Dec 1995 19:30:02 -0600
Subject: Yes I saw the "Commerical Posting"

Contents Retrieved from Microscopy Listserver Archives
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Yes Subscribers,

I have seen the "commerical" advertising posted
by Evex Analytical. Since the postings continued
after a warning from me. I have removed their address
from the server.

Nestor.....







From: EvexAnalyt-at-aol.com
Date: Wed, 20 Dec 1995 12:42:25 -0500
Subject: Re: Amray Image Archiving, etc.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

microscopy-at-sparc5.microscopy.com

Evex Analytical Instruments


Thank you for expressing interest in the VIDX Scan Active Digital Imaging
System, the Fast, High Resolution SEM Image Acquisition.

Features VIDX Scan Active Digital Imaging System,
- Menu Driven
- Quick button operation
- X-ray mapping in minutes
- Multisouces acquisition
- 12 Bit high resolution image acquisition 4096 gray Levels
- Independent contrast for each source
- User defined resolution
- Pixel-by-pixel, multi-signal digitization

Now capture up to 6 high resolution images from SEM, STEM, EDS, WDS. Images
are scanned from the SEM, directly into the PC as an image file. All images
are stored in standard TIFF format. Archive your images on your Hard Drive,
Tape Backup, CD Writer. Process your images using Image Pro, the world's
greatest image processing software. Write Reports - Import images
effortlessly into your word processor. Print to the 1200x600 Laser Printer
giving Photo realistic quality. Network your system via the PC.

You will be able to capture images quickly and economically without the use
of expensive and time consuming micrographs.

The VIDX Scan Active Digital Imaging System, actively controls the SEM Scan,
thus allowing multi-frame image integration. Multiple time constants allow
the scan to be slowed for high noise situation such as low voltage or low
spot size conditions. Images can be stored with SEM data (micron bars, user
comments) permanently overlaid on the image, or stored in the image file
without overwriting any part of the image.

The pixel-by-pixel, multi-signal digitization method will assure the
precision necessary to overlay images acquired from different sources.

If you would like to learn more about VIDX Technology products contact your
Service & Sales representative at (908)874-3800. We look forward to
satisfying your X-ray Microanalysis & Imaging needs.







From: Richard E. Edelmann :      edelmare-at-CASMAIL.MUOHIO.EDU
Date: Wed, 20 Dec 1995 15:52:27 -500
Subject: (Fwd) Re: Amray Image Archiving, etc.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


[Please, skip this message if you like, and I am sorry for filling
up your mailbox. I tried several times to reply directly to the
author with out success]

Well, me throw in my two cents worth:

} We're looking to begin electronically archiving our SEM images from
} a vintage 1988 Amray 1820. (This is digital imaging, but not PC
} controlled.)

I take it by "digital imaging, but not PC controlled, what you mean
is that you already have a bunch of digital images, but they're in
an Amray format and you are wishing to store them on a standard PC
format media, in a standard format (TIFF,PCX, GIF, etc.) yes?

Assuming I've got this correct than you have just two problems:
(1) Getting the Armay stored images onto a PC based system. This is
done either by getting a some type of drive which will read the
Amray disks and then re-save them to a PC-format disk drive, or
"networking" the Amray system to a PC. (Let me address the second
problem first and come back to this one below) (2) You have image files in
an Amray format, which will need to be comverted into a standard
image format (just about all PC based imaging software will handle a
wide number of image formats, and will easily handle conversions
between standard formats, which format you choose is of little
consideration in this situation BUT should be considered since some
formats will result in a reduction of data, i.e TIFF of an image is
not equal to JPEG of the same image). But unless you know that the
Amray images are stored in a standard format (i.e. TIFF most likely)
or someelse out there can provide you with and Amray to something
else conversion program, you will either have to write your own
conversion software or have to get this conversion software from
Amray.

Networking the Amray with a PC: This can be done a number of
different ways: (1) via an ethernet connection, (2) via a serial line
connection, or (3) via a high speed SCSI connection. And aparently
Amray suggests the SCSI connection. This is a very common PC (and
Mac.) interface, which means its commonly available and realatively
inexpensive. SCSI (pronounced Skuzzy) currently has three PC
configurations: SCSI - I, SCSI - II (Fast) , and SCSI-III (Fast &
Wide). I would guess that the Amray will handle SCSI - I, but they
are downwardly compatible, i.e. SCSI-III will handle -II & -I. Not
having talked with Amray, or having experience with this Amray
situation, I don't know exactly how they plan on handling the data
transfer but my guess is basically the Amray system (Microscope) will
be treated as simply a "device" (Like a printer, scanner or external
diskdrive) connected to the PC system via the SCSI. And you will be
able to copy data from the Amray system directly to the PC system and
store it to disk if you want. You can add SCSI to any 386 or higher
PC system (386 will only handle SCSI-I, a 486 or pentium is needed
for SCSI-II or -III). And I strongly suggest you get an Adaptec SCSI
controller (Prices range from ~$100 - $300) as I have found them to
be very reliable and very strongly support throughout the industry.
What you can do is purchase your own PC computer system, to handle
whatever you would like so long as it can also handle SCSI (~$800 for
a bare bones system, upto ~6,000 for a really great imaging system,
but you can figgure on ~$2,000-2,500 for a good solid system (I do
not recommend a notebook or laptop system for this purpose), and
have it run DOS/Windows, OS/2, Windows 95 or Windows NT (UNIX can be
a pain in the .... neck?) whatever your pleasure. And feel free to
make it networkable.

SCSI drives: Each SCSI controller card can handle upto 7 devices
connected to it (SCSI-III upto 15). Therefore you can use the SCSI
controller to connect (1) the PC hard disk, (2) the AMRAY connection,
(3) a removable storage device, and still have room for 5 more
devices (i.e. a CD-ROM drive and/or a scanner)

} About the actual mechanics of doing this, I have a couple
} questions. First, Amray recommends a system they will supply, that
} consists of a dedicated computer with normally big hard drive,
} monitor, and image transfer utility via SCSI interface. You get
} about 3 TIFF images/megabyte of hard disk storage. I'm not well
} versed in this field, but seem to recall hearing more than once
} about inexpensive magneto-optical drives and cheap, removable data
} storage disks that hold lots of images. We are PC, not Mac based,
} and are corporately networked. Is there something about our
} particular configuration, i.e., Amray to PC system, that requires us
} to go this comparatively expensive route to a dedicated computer
} with hard drive? Second, is there any way we could get more bang for
} the buck and archive images from our PLM with common hardware?


Removable drives: There are a number of options here.

(1) Magneto Optical disk drives, these can store upto 4.6Gb. Common
storage sizes are 650Mb, 1.3Gb, and 4.6Gb $800 for a 650Mb
(Panasonic), $999 for a 1.3Gb (NEC), $1700 for a 1.3Gb ( SONY or
Panasonic) to $2100- 2,600 for a 4.6 Gb drive (Prices vary on disk
size and speed for writting and reading). Disk prices vary from
$65/650Mb, $90/1.3Gb, and 4.6Gb/ ? ~$135. These drives are the
fastest, nearly as fast as current hard drives, thought they write
slower than the read. Generally the larger drives can handle the
smaller disks, but this may vary depending on price for the drive
(more $$ for more flexiblity). These disk cartridges are 1cm high
by 15cm wide. The drives are single sided, i.e you have to remove,
flip and reinsert the disk. But they are 'infinitely' rewrittable
(some are WORM - write once read many), and they are randomly
readable. I have worked with these for three years now and haven't
had many problems with them.

(2) remoavble hard disks: these can store from 44Mb (Syquest or
Bournolli), to 270Mb. Common storage sizes are 44Mb, 88Mb, 105Mb,
135Mb, 200Mb, and 270Mb. And the dirve prices vary from $239 to $600.
Disk prices vary: $50 / 44 Mb, $65/105Mb, $70 /200Mb. These are
fast, the disks don't hold much and you don't really save much $ per
disk, but they are more common to find others in your area with the
ability to read/ write with disks.

(3) CD-ROM recordables: These are strickly write-once read many
(WORM). Drive prices have fallen to ~$1000 (2x or 4x) to $3000 for
6x. The disk cost $10, and hold upto 650Mb. Adavantage: cheaper
media, VERY common reader drives, very stable for LONG term storage.
Disadvantage: problems writting disks (you would still have to copy
your data to a harddisk and ten write them to the CD-r, very slow
writting disks, slow reading disks, WORM.

(4) Zip drives: very cost effective, but very slow since the data
is compressed both during writting and reading.

(5) Tape drives: Too slow for anything but long term back up
storage.


} Apart from archiving, I'd be interested in hearing
} (especially from Amray users of older scopes) about other image
} exploits. Sending them to your customers? Printing them on laser
} printers? Any special considerations for future compatability?
} Thanks for whatever you may have to share, Dave Stadden
} DRStadde+aCENTRAL%ARMSTRONG-at-MCIMAIL.COM

There I think I have written far too much for you to consider at
present, so I'm not going to comment on anything else at the moment.
But do feel free to ask if anything I have written doesn't make
sense. I'm in the never ending process of developing a digital
imaging network here in my facility, which is why I have all the
above info in hand.

Richard E. Edelmann
Electron Microscopy Facility Supervisor
352 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513-529-5712 Fax: 513-529-4243
E-mail: edelmare-at-muohio.edu

int.




From: ROBIN CROSS :      EURC-at-giraffe.ru.ac.za
Date: Thu, 21 Dec 1995 08:34:45 GMT+0200
Subject: 6th Asia Pacific EM conference

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Anyone having difficulty reaching the organizers of this
conference using the telephone and fax numbers quoted, for
example, in the Proceedings of the RMS, should note that there
should be an extra "2" in the numbers.

Dr E.C. Chew's correct telephone and fax numbers are:

tel: +852 2609 6853
fax: +852 2603 5031


Robin Cross
Director : EM Unit, Rhodes University, Grahamstown, South Africa
eurc-at-giraffe.ru.ac.za (tel: +27 461 318168 - fax: +27 461 24377)




From: Walter Arno Mannheimer :      WAMANN-at-metalmat.ufrj.br
Date: Thu, 21 Dec 1995 08:35:37 EST3EDT
Subject: pol light pictures

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Have you looked at Tomer's book Structure of metals through optical
microscopy? The english edition is from American Society for Metals,
Metals Park Ohio, but if I recall correctly the original hebrew
edition has many more nice pictures. The ASM book quotes his
location as Nuclear Research Center Negev, Beer Sheva, Israel.
Prof.Walter A.Mannheimer
Metallurgy and Materials Engineering
Federal University of Rio de Janeiro
POBox 68505
21945 Rio de Janeiro Brazil
Voice 5521 280-7443 (Dept.office) 5521 590-0579 (direct)
Fax 5521 290-6626 Email: wamann-at-metalmat.ufrj.br




From: bell-at-medcolpa.edu (Ted Bell)
Date: Thu, 21 Dec 1995 10:39:10 -0400
Subject: background from DAB

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Does anyone have any hints on reducing the background and debris from DAB
labelling?


Ted Bell, bell-at-medcolpa.edu
Medical College of Pennsylvania and Hahnemann University
Neurobiology and Anatomy
http://ussanatomy.medcolpa.edu/FaberWeb/confocal.html






From: peggy-at-research.nj.nec.com (Peggy Bisher)
Date: Thu, 21 Dec 1995 11:10:56 -0400
Subject: Re: Subject: TEM-Bacteriophage IEM

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Message-Id: {9512211615.AA12830-at-shakti.nj.nec.com}
X-Sender: peggy-at-shakti.nj.nec.com
Mime-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: quoted-printable

}
} Does anyone know of a good, reliable way to produce images of negatively
} stained specimens after immunogold labeling? I have tried the following
} two methods with bacterophages.
}
} Method A. The protocol followed was:
} (1) Adsorb properly diluted phage sample onto glow-discharge plastic-carbon
} coated grid for one minute.
} (2) Incubate with 1st antibody for 30 minutes.
} (3) Rinse the grids with buffer, or pass the grids over drops of buffer.
} (4) Incubate with 2nd antibody-gold for 30 minutes.
} (5) Rinse again.
} (6) Negative stain.
} The difficulty with this method I have encountered is that after the
} procedure is applied either the film breaks as soon as the electron beam
} hits, or most of the phages are lost. (Without IEM, the negative-stained
} phages were fine).
}
} Method B: Incubate the phages with 1st antibody and 2nd antibody-gold in a
} microfuge tube and then negative stain. This method works well, but the
} specimen appears to have a very high background of gold particles.
}
} I wonder if others have similar difficulties and how they are dealing with
} them. Any information or suggestions would be appreciated.
}
} Eleana Sphicas
} Rockefeller University



I have had sucess in the past with a slight variation of method B:

My antibody-labeled sample was incubated with Protein A-gold for 60 =
min
at 20=B0C, and then separated from the unbound labed by wasing (four cycles)
in phosphate-buffered saline, employing a Beckman airfuge centrifugation
conditions of 134,000g with rotor 95A or at 196,000g in the 110A rotor.

The final pellet was taken up in 10=B5l of buffer and then negativel=
y
stained for electron microscopy.


Ref: J. of Struct. Biology, 106: 221-236 (1991)


Good Luck,



****************************************************************************

Margaret(Peggy) Bisher

NEC Research Institute _/ _/ _/_/_/ _/_/_/ _/
4 Independence Way _// _/ _/ _/ _/
Princeton, NJ 08540. _/ / _/ _/_/_/ _/ _/
Tel.: (609) 951-2629 _/ /_/ _/ _/ _/
=46ax: (609) 951-2496 _/ _/ _/_/_/ _/_/_/ _/
e-mail: peggy-at-research.nj.nec.com


****************************************************************************









From: semlab-at-Sparc5.Microscopy.Com
Date: Thu, 21 Dec 1995 13:18:44 +0000
Subject: LM - Need help on solutions with volatile solvents

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Message-Id: {199512211718.LAA21069-at-Sparc5.Microscopy.Com}
Comments: Authenticated sender is {semlab-at-mail.ims.uconn.edu}

Hi! I'm a Polymer Science student, and I would like to know which LM
techniques are used to prevent evaporation of volatile solvents from
solutions. Special slides & cover slips? Special sealing waxes? Thin
cuvettes with Teflon stoppers? Other ideas? Solvent example:
heptane.

I'd greatly appreciate your suggestions! Happy Chanukah and Merry
Christmas to you all!

Sincerely, UConn/IMS SEM Lab (from David J. Moonay)




From: Rex Holland :      rholland-at-umich.edu
Date: Thu, 21 Dec 1995 13:53:47 -0500 (EST)
Subject: Freezing tissue

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Message-Id: {199512211853.NAA17092-at-gamera.rs.itd.umich.edu}

We have recently experienced some problems with frozen tissue samples being
damaged at some point before they are sectioned. We are looking at teeth
that have been fixed in aldehydes then decalcified in formic acid/formate
before freezing and sectioning. I suspect that the culprit is either the
freezing procedure or the storage of the frozen blocks. The tissue that has
disappointed us was frozen in OCT slowly in the cryostat and then stored
there. I realize that for unfixed tissue 'snap' freezing is usually
advocated but thought that gradual freezing was kinder if the tissue had
been stabilized by chemical fixation. I realize this is a nieve point but we
may be missing something obvious. I would appreciate hearing what other
people use even if it is mundane and routine.

Rex Holland





From: Glen Macdonald :      glenmac-at-u.washington.edu
Date: Thu, 21 Dec 1995 11:14:38 -0800 (PST)
Subject: Re: background from DAB

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X-Sender: glenmac-at-homer24.u.washington.edu

Ted,
How about providing some specifics about your immunolabelling
protocol and sample type? There are a number of ways to get background.


Glen MacDonald
Research Scientist
Hearing Research Laboratories of the
Virginia Merrill Bloedel Hearing Research Center
Box 35-7923
University of Washington
Seattle, WA 98195-7923
(206) 616-4156
glenmac-at-u.washington.edu



On Thu, 21 Dec 1995, Ted Bell wrote:

} Does anyone have any hints on reducing the background and debris from DAB
} labelling?
}
}
} Ted Bell, bell-at-medcolpa.edu
} Medical College of Pennsylvania and Hahnemann University
} Neurobiology and Anatomy
} http://ussanatomy.medcolpa.edu/FaberWeb/confocal.html
}
}
}




From: Fermin, Cesar :      fermin-at-TMC.Tulane.Edu
Date: 21 Dec 1995 15:00:30 -0500
Subject: New Web Page

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Message-Id: {n1392564027.15872-at-msmail.tmc.tulane.edu}

A newly created web page for activities of the Fermin laboratory at Tulane
Department of Pathology is now available:
http://www1.omi.tulane.edu/departments/pathology/fermin/cdftop.html
Send your feed back. Thanks, Cesar




From: EmLab :      EMLAB-at-OPUS.MCO.EDU
Date: Thu, 21 Dec 1995 16:32:47 -0500 (EST)
Subject: Re: Freezing tissue

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Rex,

What is the damage and which type of microscopy are you doing?
The problem might be the formic acid solution, very very rough on tissues.
Is the calcium all removed?

Best of luck
Ed Calomeni
Medical College of Ohio
Toledo, OH
emlab-at-opus.mco.edu




From: Chris Frethem (CBN) :      frethem-at-lenti.med.umn.edu
Date: Thu, 21 Dec 1995 16:40:59 -0600 (CST)
Subject: unsubscribe

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unsubscribe frethem-at-lenti.med.umn.edu



=======================================================================
Chris Frethem (612)624-4652 (voice)
Cell Biology & Neuroanatomy (612)624-8118 (FAX)
U of MN, Minneapolis e-mail: frethem-at-lenti.med.umn.edu







From: Donald P Robertson :      donald-at-csd.uwm.edu
Date: Thu, 21 Dec 1995 17:21:52 -0600 (CST)
Subject: unsubscribe to newsgroup

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unsubscribe Donald P. Robertson {donald-at-csd.uwm.edu}





From: EvexAnalyt-at-aol.com
Date: Thu, 21 Dec 1995 18:53:36 -0500
Subject: OOPS!!! Apologies From Evex Analytical

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We at Evex apologize for the mail sent to the list-server.

Brian Crammer
Systems Administrator






From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 21 Dec 1995 15:17:07 -0400
Subject: RE- Texture of Au Leaf

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Message-ID: {n1392563254.2097-at-mse.engin.umich.edu}

Subject: Time: 3:07 PM
OFFICE MEMO RE: Texture of Au Leaf Date: 12/21/95

The book "Theory and Practice of Electron Diffraction" by Thomson and
Cjochrane, Macmillan, 1939 contains a considerable amount of discussion on
electron diffraction patterns obtained from gold leaf.
W. C. Bigelow (bigelow-at-umich.edu)





From: minter-at-kodak.com (John Minter)
Date: Thu, 21 Dec 1995 15:16:29 -0500
Subject: SUMMARY: Section Collection Loops

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Last week I posted a request for information about a new style of
loop for the collection of ultrathin sections. Many thanks to all
who responded. I was particularly impressed when I received a phone
call from one vendor who recognized that I was decribing a competitor's
product and phoned with the information anyway. Below is a summary of the
information I received.

Many of you reported that I was recalling the "Perfect Loop (TM)"
from Electron Microscopy Science. (I think I'm OK in mentioning
the trade name, I have no commercial interest in EMS and, by the way,
I have deleted price info from the summaries below.) Others suggested
using slot grids. We will try both approaches and see which is best...

Jean-Louis Lavergne wrote:
} I'm using a perfect loop from Electron Microscopy Science. It size fits
} perfectly with the grid diameter. I'm not sure that this "concentrate"
} the sections to the center but the meniscus formed during the picking of
} the section is helpful for "destressing" room temperature sections.
}
} This instrument is really nice to have if you have to collect Room temp
} sections. For cryo work I've never try it.

Scott Walck {walcksd-at-ml.wpafb.af.mil} wrote:
} Electron Microscopy Sciences sells them. Talk to Stacie Kirsch. I have one
} and they work well for what I do. I collect films that I float off NaCl
} crystals. If you like, I can let our microtomist try it out and she can get
} back to you

John. J. Bozzola {bozzola-at-siu.edu} wrote:
} Could you be referring to the "Perfect Loop" made by Electron Microscopy
} Sciences (215-646-1566). [price info deleted] I have used one and they
} work fairly well on uncoated grids. They're almost perfect but we usually
} pick up grids onto slots and then deposit the grids with sections floating
} on the retained droplet onto Butvar films.

Mike Veith {veith-at-wustlb.wustl.edu} wrote:
} ... I use the EM version and never section without
} it. It's the next best thing to sliced bread--in my opinion! It does what
} the vendor says it will.

And thanks for similar information from rw9-at-psu.edu (Rosemary Walsh) and
Patty Jansma {plj-at-manduca.neurobio.arizona.edu} for info similar to the above.

Paula Falk (pmf-at-avery.med.virginia.edu) wrote:
} I did not see the advertised product. I have always used slot
} grids with on corner folded up for easier pickup. The slots
} concentrate the sections in the middle on mesh grids, and with
} the aid of an eyelash they can be aligned to the filmed slot grid
} so all sections are on the slot. This also prevents stress on
} filmed slot grids because they don't have to be submerged in the
} boat.

And Chris Pella also wrote to suggest slot grids, passing on
K. Chien et al., "A simple procedure for obtaining clean sections
for TEM," 42nd EMSA Meeting, pp. 42 (1984).






From: Glen Macdonald :      glenmac-at-u.washington.edu
Date: Thu, 21 Dec 1995 16:08:02 -0800 (PST)
Subject: Re: Freezing tissue

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X-Sender: glenmac-at-homer30.u.washington.edu

Rex,
Snap freezing will give better results even for fixed tissue.
Fixation usually will further improve morphologic detail over that
obtained with unfixed samples because of greater resistance to
crystalization of fwater. Slow freezing allows formation of more and
larger ice crystals within the tissue. For example, slow freezing of
vibratome sections helps antibody penetration by ripping big holes in the
membranes.
Although formic acid solutions are
better than other acids for preservation of detail, you might experiment
with warm EDTA for better results. Further discussion of decalicification
techniques may be found in "Preparation of Decalcified Sections" by Edward
B. Brain, pub. by Charles C. Thomas.


Glen MacDonald
Research Scientist
Hearing Research Laboratories of the
Virginia Merrill Bloedel Hearing Research Center
Box 35-7923
University of Washington
Seattle, WA 98195-7923
(206) 616-4156
glenmac-at-u.washington.edu


On Thu, 21 Dec 1995, Rex Holland wrote:

} We have recently experienced some problems with frozen tissue samples being
} damaged at some point before they are sectioned. We are looking at teeth
} that have been fixed in aldehydes then decalcified in formic acid/formate
} before freezing and sectioning. I suspect that the culprit is either the
} freezing procedure or the storage of the frozen blocks. The tissue that has
} disappointed us was frozen in OCT slowly in the cryostat and then stored
} there. I realize that for unfixed tissue 'snap' freezing is usually
} advocated but thought that gradual freezing was kinder if the tissue had
} been stabilized by chemical fixation. I realize this is a nieve point but we
} may be missing something obvious. I would appreciate hearing what other
} people use even if it is mundane and routine.
}
} Rex Holland
}
}




From: Richard Thrift :      Richard_Thrift-at-depotech.com
Date: Thu, 21 Dec 1995 19:05:51 -0800
Subject: LM: Particle sizing advice

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Message-Id: {s0d9b021.061-at-depotech.com}
X-Mailer: Novell GroupWise 4.1

Hi
We usually use laser diffraction to determine the size distribution of our
product, which is a suspension of roughly 15 micron diameter (range 5
to 50 or more microns) translucent spheres in saline. I want to use light
microscopy in order to verify that instrument's accuracy. But it seems
that the errors in microscopy are almost as large as the error of the laser
diffraction instrument. Has anyone found a good solution to this?

My problem is basically that I want to measure particles that have a
range of diameters, and that have settled on to the surface of the glass
slide, so the equators are all at different heights above the slide. I've
been using the 40x objective in brightfield mode with the condenser
aperture stopped down to keep the depth of field greatest, but just now
realized what that (N.A. 0.2) does to the resolution. Guess I'll have to
take a huge number of pictures with the 100x oil objective & aperture
open. Still, if I have several particles in each field, on average it will be
rare to have the equator in focus. I'd rather not spend time working this
out if it has already been done. Any suggestions?

The translucent particles act as lenses, so the apparent diameter is
usually larger than the actual diameter, and I'm hoping to be very
accurate. For instance, Duke latex beads 15.03 microns diameter (R.I.
~1.5) appear 16 microns if I measure individual particles, though a chain
of 10 beads (to minimize error per bead) gives 15.1 microns per bead.
And this is with the particles all in focus ! (The high R.I. seems to
exacerbate the problem.) The apparent diameter seems to vary with
refractive index, radius of curvature, and whether the plane of focus is
above, at, or below the particle's equator. I'm interested not only in
mean size, but also in the shape of the distribution out in the tails. Any
suggestions? Have I reached the limits of the technique? For that
matter, can anyone tell me how to calculate how many particles I need to
measure in order to get acceptible statistical error in a given size interval
out in the tail of the size distribution?

Please respond directly to me.
Thanks!
Richard_Thrift-at-Depotech.com





From: David R Stadden at icnorth 1995/12/14 15:54
Date: 14/12/95 4:03 PM
Subject: Amray Image Archiving, etc.

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Although I've inquired on this subject before, things change, and I'm
wondering what may be new. We're looking to begin electronically archiving
our SEM images from a vintage 1988 Amray 1820. (This is...






From: fahayes-at-ucdavis.edu (Fred Hayes)
Date: Thu, 21 Dec 1995 21:15:03 -0800
Subject: Texts for confocal and cryomicrotomy

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Can anyone recommend introductory texts (theory, application and technique)
for confocal microscopy and cryomicrotomy ?

Thank you,

Fred A. Hayes
916-678-6280





From: Paul Webster :      Paul.Webster-at-quickmail.yale.edu
Date: 22 Dec 1995 00:58:10 -0500
Subject: Re: Freezing Tissue

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Message-Id: {n1392528295.68340-at-QuickMail.Yale.edu}

Subject: Time: 12:36 AM
OFFICE MEMO Re} Freezing Tissue Date: 12/22/95

Although I am not an expert in the theory and methods of freezing biological
tissues, I do know that the techniques used to prevent ice crystal formation are
not trivial.

Vitrification (freezing without ice crystal formation), although theoretically
simple, is not easy to achieve in practice if cryoprotectants are not used. The
theory is to remove the heat so fast that the water has no time to form a
crystal. To remove the heat this fast requires special equipment and very small
specimens. Snap freezing, which I assume means plunging a specimen into a
beaker of liquid nitrogen or pentane, is not usually sufficient to freeze large
histological specimens without ice crystal damage. Neither will fixing the
specimen prevent ice crystal damage.

The simplest solution for preventing freezing damage in routine histological
specimens, which are to be sectioned in a cryostat, is probably to cryoprotect
the fixed material before plunging in liquid nitrogen. Tokuyasu has shown that
sucrose solutions that are over 1.6 M can be frozen by immersion in liquid
nitrogen without ice crystal damage.

As an aside, I thought that the discussion about being able to quantify the
number and size of ice crystals in frozen tissue was effectively closed in the
1980's by the work of Dubochet, Unwin and others.

My advice to any histologist worried about the enormous tissue damage that
occurs when freezing fixed or unfixed specimens in cryogenic liquid, is to
cryoprotect before freezing, then freeze by immersion in liquid nitrogen.
Sucrose will easily penetrate fixed tissues but should not be used as a
cryoprotectant for unfixed material.





From: Luc.Brohan-at-cnrs-imn.fr (BROHAN)
Date: Fri, 22 Dec 1995 10:52:59 GMT
Subject: H.R.T.E.M camera/ Material Science

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Help,

We are interesting by a camera equipment for HITACHI H9000
NAR in order to perform High resolution in solid state chemistry
area.
Could you send me your user opinion about TV rate
intensified or Slow Scan camera such GATAN or LHESA and so on (
Resolution, FFT convenience, performance...).
TV rate:
GATAN 622, 692, 672
LHESA LH72LL
SLOW scan:
LHESA
GATAN
Thank you
Sincerely your,

A. MARIE, M. Caldes RICOS and L. BROHAN




From: dietmar.reiter-at-uibk.ac.at (Dietmar Reiter)
Date: Fri, 22 Dec 1995 12:56:17 +0100
Subject: Re: Texts for confocal and cryomicrotomy

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} Can anyone recommend introductory texts (theory, application and technique)
} for confocal microscopy and cryomicrotomy ?

Regarding Confocal microscopy I recommend the second edition of the
*Handbook of biological confocal microscopy* (Editor: James Pawley; Plenum
Press, N.Y., 1995). Probably far beyond an introductory text, it is a
really comprehensive collection of all topics regarding confocal
microscopy.

-Dietmar-

+++ Dietmar Reiter {dietmar.reiter-at-uibk.ac.at} ++++++++++++++++
+++ Dept. of Zoology and Limnology, University of Innsbruck ++++
+++ Technikerstrasse 25, A - 6020 Innsbruck, Austria +++++++++
+++ ph: (+43)-512-507-6170 (-6161), fax: ..-507-2930 (-2957) +++






From: Warren Straszheim :      wesaia-at-iastate.edu
Date: Fri, 22 Dec 1995 08:58:21 -0600
Subject: Visilog and/or Link ISIS users' group?

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Does anyone know of a formal or informal users group for either the Visilog
image analysis program (under Windows in particular) and/or the Link ISIS
EDS system?

I am beginning some image analysis in earnest under the Visilog system on a
Link and have run into a number of difficulties. Things aren't always
working as I think they ought. While I think know image analysis, I
definitely do not know its implementation on this software. Maybe I am
misunderstanding the documentation.

I am also particularly interested in developing a custom script or scripts
so that we can have a fairly automatic system. Anyone out there with
experience in this area?
----------------------------------------------------
Warren E. Straszheim
270 MD Ames Lab/ISU, Ames IA, 50011
Phone: 515-294-8187 FAX: 515-294-3091
E-Mail: wes-at-ameslab.gov (or: wesaia-at-iastate.edu)

coal characterization and processing
electron microscopy, x-ray analysis, image analysis
computer applications





From: Glen Macdonald :      glenmac-at-u.washington.edu
Date: Fri, 22 Dec 1995 09:27:20 -0800 (PST)
Subject: Re: Texts for confocal and cryomicrotomy

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As Dietmar says, Dr. Pawley's book is an excellent reference. For a more
practical approach, see Methods in Cell Biology, Vol. 38, edited by Brian
Matsumoto, and for theory, Confocal Microscopy, edited by T. Wilson
(Academic press) is a good choice.


Glen MacDonald
Research Scientist
Hearing Research Laboratories of the
Virginia Merrill Bloedel Hearing Research Center
Box 35-7923
University of Washington
Seattle, WA 98195-7923
(206) 616-4156
glenmac-at-u.washington.edu



On Thu, 21 Dec 1995, Fred Hayes wrote:

} Can anyone recommend introductory texts (theory, application and technique)
} for confocal microscopy and cryomicrotomy ?
}
} Thank you,
}
} Fred A. Hayes
} 916-678-6280
}
}




From: Raymond F Egerton :      egerton-at-phys.ualberta.ca
Date: Fri, 22 Dec 1995 11:21:10 -0700 (MST)
Subject: Si TEM grids

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THANKS to all who responded to my queries about use of Si-membrane grids.
I will discuss the results with AMC and provide individual answers (in
January) to some of the questions raised. Ray Egerton.






From: george.braybrook-at-UAlberta.CA (George Braybrook)
Date: Fri, 22 Dec 1995 14:52:37 -0700
Subject: newsgroup subscription

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Merry Christmas

Please sign me up to this newsgroup.

Cheers
:)
George
Department of Earth and Atmospheric Sciences
University of Alberta
Edmonton, Alberta T6G 2E3
Canada
ph: 403-492-5746
fax: 403-492-2030






From: rw9-at-psu.edu (Rosemary A. Walsh)
Date: Fri, 22 Dec 1995 10:31:12 -0500
Subject: Re: LM: Particle sizing advice

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microscopy-at-Sparc5.Microscopy.Com

Dear Richard,
Do you have the possibility of checking your diffraction
and LM particle size distributions with measurements taken from either
digitized SEM photomicrographs or from SEM images collected and measured
with image analysis software? Scored sample holders or finder grids are
aids to insure the scanning of the area covered by sample particles. I
generally use this method when checking on volumetric distributions. I
would fix,dehydrate, critical point dry and sputter-coat "wet" spheres
for examination in a conventional SEM. Other possibilities include the use
of a CRYO-SEM or a Wet SEM.
Rosemary






At 7:05 PM 12/21/95 -0800, Richard Thrift wrote:
} Hi
} We usually use laser diffraction to determine the size distribution of our
} product, which is a suspension of roughly 15 micron diameter (range 5
} to 50 or more microns) translucent spheres in saline. I want to use light
} microscopy in order to verify that instrument's accuracy. But it seems
} that the errors in microscopy are almost as large as the error of the laser
} diffraction instrument. Has anyone found a good solution to this?
}
} My problem is basically that I want to measure particles that have a
} range of diameters, and that have settled on to the surface of the glass
} slide, so the equators are all at different heights above the slide. I've
} been using the 40x objective in brightfield mode with the condenser
} aperture stopped down to keep the depth of field greatest, but just now
} realized what that (N.A. 0.2) does to the resolution. Guess I'll have to
} take a huge number of pictures with the 100x oil objective & aperture
} open. Still, if I have several particles in each field, on average it will be
} rare to have the equator in focus. I'd rather not spend time working this
} out if it has already been done. Any suggestions?
}
} The translucent particles act as lenses, so the apparent diameter is
} usually larger than the actual diameter, and I'm hoping to be very
} accurate. For instance, Duke latex beads 15.03 microns diameter (R.I.
} ~1.5) appear 16 microns if I measure individual particles, though a chain
} of 10 beads (to minimize error per bead) gives 15.1 microns per bead.
} And this is with the particles all in focus ! (The high R.I. seems to
} exacerbate the problem.) The apparent diameter seems to vary with
} refractive index, radius of curvature, and whether the plane of focus is
} above, at, or below the particle's equator. I'm interested not only in
} mean size, but also in the shape of the distribution out in the tails. Any
} suggestions? Have I reached the limits of the technique? For that
} matter, can anyone tell me how to calculate how many particles I need to
} measure in order to get acceptible statistical error in a given size interval
} out in the tail of the size distribution?
}
} Please respond directly to me.
} Thanks!
} Richard_Thrift-at-Depotech.com






From: mgj-at-csd.uwm.edu (Marija Gajdardziska-Josifovska)
Date: Fri, 22 Dec 1995 15:15:08 -0600 (CST)
Subject: unsubscribe to newsgroup

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_________________________________________________________________________
Marija Gajdardziska - Josifovska
AssistantProfessor
Department of Physics
University of Wisonsin Milwaukee
P.O.Box 413
Milwaukee, WI 53201
phone: (414) 229 4965
Fax: (414) 229 5589






From: Paul Webster :      Paul.Webster-at-quickmail.yale.edu
Date: 22 Dec 1995 16:52:26 -0500
Subject: Re: Texts for confocal and R

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Message-Id: {n1392470935.16806-at-QuickMail.Yale.edu}

Subject: Time: 4:43 PM
OFFICE MEMO RE} Texts for confocal and RE} Texts... Date: 12/22/95

The question about what is a useful introductory text for cryomicrotomy has two
answers. The first is if the cryomicrotomy is to be performed on unfixed,
vitrified material. A useful introductory text can be found in the Royal
Microscopy Society Handbook series (number 21, I think). The authors are Roos
and Morgan and they thoroughly cover the basic theory and practical details of
rapid freezing, vitrification and sectioning.

There are also two chapters covering the second subject, cryomicrotomy of fixed,
cryoprotected material for immunocytochemistry. However, the best reference
work on immunocytochemical methods is still the Griffiths book (1993, Springer
Verlag, "Fine Structure Immunocytochemistry").

An even better way of picking up this technique is to attend a course (or visit
a laboratory) specializing in cryosectioning.

Good Luck and Happy Holidays.
Paul Webster, Ph.D.
Center for Cell Imaging
Yale University School of Medicine.





From: Dana T Nojima :      Dana.T.Nojima-1-at-tc.umn.edu
Date: Fri, 22 Dec 1995 18:16:53 -0600 (CST)
Subject: unsubscribe

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From: ARGIL
Date: Fri, 22 Dec 1995 23:57:42 -0500 (EST)
Subject: Stereo Microscopes

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We import microscopes from China. They are not used, but high quality
at pretty low prices.

At this time, we have quite a few stereos, including single power,
dual power, and zooms. We have a few
demo models with some discount at this time of year.

Please contact us if your colleague has any interest in more information.

Arthur Gillman
Argyle International Inc
Princeton, NJ

We import microscopes from China. They are not used, but high quality
at pretty low prices.

At this time, we have quite a few stereos, including single power,
dual power, and zooms. We have a few
demo models with some discount at this time of year.

Please contact us if your colleague has any interest in more information.

Arthur Gillman
Argyle International Inc
Princeton, NJ




From: d-hall-at-ix.netcom.com (Don Hall )
Date: Fri, 22 Dec 1995 22:37:41 -0800
Subject: hu=12 hitachi electron micro. document help

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I was at a government sale and in the process of bidding on
'lots' of goods I aquired an Hitachi HU-12 electron microscope. It is
super heavy, but I managed to get it into my shop. It apears to have
tipped over at some point in it;s life and a few adjustment knobs are
bent/broken. What I would like to find is documentation on it. I went
through a telephone marathon only to find a guy that had it but would
not help? Can anyone out there give me any assistance. I would like to
make the thing operational as a diversion from .... Thanksin advance for
any help or clues to find help.
Don Hall





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Date: Sun, 24 Dec 1995 10:35:39 -0500
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From: Jane A. Fagerland (708) 935-0104 :      FAGERLAND.JANE-at-igate.pprd.abbott.com
Date: Tue, 26 Dec 1995 10:12:00 -0600 (CST)
Subject: Freezing tissue

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30% sucrose in PBS is a good cryoprotectant for lungs and would probably
work for hard tissues, also. Fix the tissues first, then remove
fixative in three changes of PBS (10 minutes each for lungs, probably
longer for your tissue). Soak tissues in cryoprotectant at least
overnight, at 4C. Blot off excess liquid, then snap-freeze the tissues
in embedding medium. I found that tissues embedded in Lipshaw's medium
were easier to section than those embedded in OCT. You might give the
Lipshaw medium a try. I also concur with the comment about your decal
solution - I think EDTA is considered to be a "kinder and gentler"
decalcifying agent than acid treatments.

Good luck!

Jane A. Fagerland, Ph.D.
Dept. Cellular and Microscopic Research
Abbott Laboratories
Abbott Park IL 60064






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Date: Wed, 27 Dec 1995 09:25:30 -0600 (CST)
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Date: Thu, 28 Dec 1995 08:56:10 -0500
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From: mdavey-at-islandnet.com (Mike Davey)
Date: Sun, 31 Dec 95 07:31 PST
Subject: LM - staining of mushroom gills, tissue

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