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Email: mlynn-at-ameslab.gov Name: Matthew Lynn
Organization: Ames Laboratory, Dept. of Energy
Title-Subject: [Filtered] TEM: Postdoctoral position at the Ames Laboratory
Message: The Division of Materials Science and Engineering (DMSE) at the Ames Laboratory, a Department of Energy National Laboratory affiliated with Iowa State University, is searching for a qualified Postdoctoral Research Associate.
This is an opportunity to support a diverse range of research programs using advanced aberration-corrected STEM and associated techniques. More details and application instructions can be found here:
From fadecice330ojoi-at-gmail.com Mon Jan 6 07:32:29 2020 Return-Path: {fadecice330ojoi-at-gmail.com} Received: from gmail.com (a3.68474.cn [23.228.73.183] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id 006DWSaq013173 for {microscopylistserverarchive7-at-microscopy.com} ; Mon, 6 Jan 2020 07:32:28 -0600 Message-ID: {029F40E5.7510914E-at-gmail.com}
Dear Colleagues,
It's the beginning of the new year and it's time to plane your future training opportunities!
We are excited to be offering the first, of what promises to be annual, Big Data, Big Problems light-sheet workshop, held at Princeton University, Princeton, NJ, USA.  The course will run July 27-July 31, 2020.
Applications are due April 15, 2020.  Apply through the course web site at BigDataBigProblems.com.
Multi-dimensional live and fixed microscope data can be collected in so many ways, and the various solutions seem to grow almost daily.  There are many courses that focus on general optical principals and the use of conventional microscope platforms such as widefield fluorescence, confocal and multiphoton imaging, but none that are specifically designed to demystify rapidly evolving and increasingly prescient methods such as light-sheet imaging.  Ultimately this is the goal of this course, what is light-sheet microscopy in all its flavors, what can you do with it, how do you choose between platforms and once you have a system or are proficient with a device what do you do with the data?  This week long course brings together experts in conventional optics, all aspects of light sheet microscopy and image analysis to help you bring your light-sheet based cellular, animal and large sample/cleared sample imaging to the next level.  From the syllabus you will see that we start out with principals and choices and then move into specific systems instructed by both academic and industry faculty.  In fact, we have will have almost all the available commercial solutions on site for you to use.  We will provide samples, but equally you are welcome to use your own.   Our goal is that you will return to your home institution fully capable to implement and use these truly exciting new tools.
Course Speakers;
Amy Elliot National Institutes of Health
Holly Gibbs Texas A&M
Elizabeth Hillman Columbia University
Jan Huisken Morgridge Institute for Research
Gary Laevsky Princeton University
Talley Lambert Harvard University
Wesley Legant University of North Carolina
Paul Maddox University of North Carolina
Alison North The Rockefeller University
Eszter Posfai Princeton University
Doug Richardson Harvard University
Kelly Seagraves Princeton University
Hari Shroff National Institutes of Health
Claudette St. Croix University of Pittsburgh
Sebastian Streichan University of California Santa Barbara
Jared Toettcher Princeton University
Simon Watkins University of Pittsburgh
  Applications are due April 15, 2020.  Apply through the course website, BigDataBigProblems.com
  Course Directors
Gary Laevsky, Princeton University
Simon Watkins, University of Pittsburgh
--
Best,
Gary Laevsky, Ph.D. Director, Confocal Imaging Facility Nikon Center of Excellence Co-Founder, North Atlantic Microscopy Society (NAMS)  https://namsmicroscopy.com/ Dept. of Molecular Biology Washington Rd. Princeton University  Princeton, New Jersey, 08544-1014 (O) 609 258 5432 (C) 508 507 1310
North Atlantic Microscopy Society Spring Meeting at UPENN, April 23, 2020.
==============================Original Headers============================== 41, 37 -- From gary_laevsky-at-yahoo.com Mon Jan 6 07:51:12 2020 41, 37 -- Received: from sonic315-13.consmr.mail.bf2.yahoo.com (sonic315-13.consmr.mail.bf2.yahoo.com [74.6.134.123]) 41, 37 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 006DpBht013837 41, 37 -- for {microscopy-at-microscopy.com} ; Mon, 6 Jan 2020 07:51:12 -0600 41, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1578314901; bh=rX7HfAq0Y2/NoJUTDdnvnFAviZgA0eej4+RFezJCCeM=; h=Date:From:To:Subject:References:From:Subject; b=OXcV0x0ZCFfA6d317PBpVbvWlhbevchds3nTIafEqV6Y9f6mpKf/Iz9s7GmpDaU3OKzd78jAeiFGdxWI60CTML4aKcLsI7TPVHwVrtRBD8cVKkgu5c2woinJ0pec6JZptOz0NHxzLpE8J5ZtpkcWgyOViUuOu5zbTYj0uXymAOg/YJLUqO+fPKg9aKFegW9LSuNHCgOisO7jGtpJMxwJ1w66X+AlO0V3cPWf8GqX+oTRXsOwxajyJZOqHgl2VJRvwCmF9Iia5nraq+S5I6A+nsKvR+zEZ3yWub+zcPq8M+1RQpyHvtZf7lgb1mRPYCbDqDEHyrqSUaPGDBEQpLaaKg== 41, 37 -- X-YMail-OSG: mdZJxeAVM1nFjQCN23ybY5ckxENFiF1gEzEVsHJ2ea2bEkxL874ESTqP9oKpld2 41, 37 -- w9VpANKCGFxTiS2ZQOlrG8ETv3qE_LCSFxjbk1.Ig_crPYbLYkQ83MocVZx198O7KK6XELM4nao0 41, 37 -- 5kAeFRJrImnq9jkEoeLNtEcffzXRywTAbXJASoMqXvET8mEySlzO9rXoo5eAhmXQc3x7ptCrIN0c 41, 37 -- 4EwMVdVtVquE8lAxuefUeUafQatqiAUYl10XabTWP3vH3xebv.uVaa6CsXQyAtwi.9UQyfgV6QHZ 41, 37 -- m959uGbqGvuIs2b9IgAgEYslI2zCR2QGxvkwrqvxj.ygkJ4o0a9vD.Bk07VL20hzPAEYyYywHJY. 41, 37 -- HLInwIrC8BT8jyksQLaGu7LNmgpAuANHEh1.se0tFVgxNCSS3uJpKwco9b3LgIFHkH5MiDMLJ_rs 41, 37 -- .sDUcyQPi7yIghNsXpdtO0IFgcaF78SgjnMFD5GCrjsqSlk7cedqLz_GxDRI1ooWPq2_0ejMODOT 41, 37 -- KUxSIf90r9fcy57iFhHOog.u.jdCh_gUgoTQEXzre.KC0lhrwnJfKjci0Lw3TzkD3uHBrRdl6YaI 41, 37 -- o_umD6xk9kz6WicbUY83kazuuzJq_i5IyvgLoM319E3DJPNwAR8YmDxSP.N96YWH76XmP7U5QsuP 41, 37 -- VrA9KEGH24cpNqjvj4xmYTjWSYpPFGAhz13cnQ8UcCm4It9_qbvbfa9pVn76_XvUMieCYyUalPbx 41, 37 -- jExCWiDMsyvEVYN0ZGXhVMWmoKUsqcok6IARB_bHtIsTHPQV85ImfqGUZqO8USy.v2.w2FIGxxwC 41, 37 -- X8R8YAtkGjv5H4la6ZGxxxgpfyV90qyeX65wiRLq6z3wKmFhMzM2rPeE87JLlmXL_jNH459RQx1u 41, 37 -- 7x.8tR7O6nJI6L8aEdmrCziP32im5FjfEYKWZvBcsYnFO7eMlWAKk.jypbH6r4RoFfI928JiX7E8 41, 37 -- FVOSbKhXymt5Wk0W9KHX5Fy26TjnAALu36y_G4VhZprobUC7vXuBxoD_i19TbOqHuZRdMfCAVOVu 41, 37 -- l7XH4ni_Vm7BDXIso81d6aDb15HVQIkrS6lHhvnNWH7eExpednpUd2.QrlW1Pn5pSqKqw8OcT_eo 41, 37 -- IKRkHieQKoMltE2JI6tRJI3xvD8VoqmHUJdDUTIz3GFOrJoc69_p5kKnNmW1AHcCiUnuYUKKqtAx 41, 37 -- 2ZOntyh9rOxfPNzi0hjGWHgm1Q.3aOKif1Bg2VITpdMFHTlGWN8rtvHfIDp1g_sgEfvu93LuUjvC 41, 37 -- ZV8FO38msGslorEusPO0tOt02.d3D6lPttfAeg3.hQZpfnzRvSsWrDxqp9sXLZ.QqcDw2NYts0n7 41, 37 -- xmJ4llhZpeOhApgAdNXUG 41, 37 -- Received: from sonic.gate.mail.ne1.yahoo.com by sonic315.consmr.mail.bf2.yahoo.com with HTTP; Mon, 6 Jan 2020 12:48:21 +0000 41, 37 -- Date: Mon, 6 Jan 2020 12:48:19 +0000 (UTC) 41, 37 -- From: Gary Laevsky {gary_laevsky-at-yahoo.com} 41, 37 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 41, 37 -- Message-ID: {1153505689.4734631.1578314899793-at-mail.yahoo.com} 41, 37 -- Subject: Big Data, Big Problems; Light-sheet workshop Princeton University, 41, 37 -- July 27-31, 2020 41, 37 -- MIME-Version: 1.0 41, 37 -- Content-Type: text/plain; charset=UTF-8 41, 37 -- References: {1153505689.4734631.1578314899793.ref-at-mail.yahoo.com} 41, 37 -- X-Mailer: WebService/1.1.14873 YMailNorrin Mozilla/5.0 (Macintosh; Intel Mac OS X 10.13; rv:71.0) Gecko/20100101 Firefox/71.0 41, 37 -- Content-Transfer-Encoding: 8bit 41, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id 006DpBht013837 ==============================End of - Headers==============================
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Title-Subject: [Filtered] question about fib milling parameters on polymer membrane
Message: Dear Colleagues,
First at all, Happy New Year !
then, I have a question for you : i started my phd in last october and i work on polymer membrane. My goal is to image, in a fist time, the 3D structure of my membrane. For this, i want to use the FIB/SEM available in my lab. I know this is fragile sample and i need to adapt my parameters for milling my surface without damage them.
This is my question : Someone alrady use this technique on polymer membrane ? And have an idea of optimal parameters (current, energy, deep, dual time..) for use the ion beam ? I have PES and PAN membranes. I need reference parameters for this type of sample.
Thanks a lot and have a nice day
Best regards
HÈlËne Roberge Phd student IMN - Institut des MatÈriaux Jean Rouxel 2, rue de la HoussiniËre - BP 32229 44 322 NANTES CEDEX 3 https://www.cnrs-imn.fr/ 0240376316
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I’m excited to announce that I’ll be hosting the spring 2020 meeting of the North Atlantic Microscopy Society (NAMS) here at the Perelman School of Medicine at the University of Pennsylvania on April 23rd.
NAMS was founded by Gary Laevsky and Paul Shao at Princeton University. Its mission is to promote microscopy education and to provide researchers and vendors in the region with opportunities to connect and hear about the latest applications of microscopy techniques. 
The focus of this meeting will be Correlative Light-Electron Microscopy (CLEM). Light and electron microscopy (EM) speakers will highlight their respective techniques, while CLEM speakers will demonstrate the benefits of bringing the two modalities together.
You can register for the meeting here: https://namsmicroscopy.com/meeting-registration
I hope to see some of you here in April!
Andrea
Andrea L. Stout, Ph. D. Director,  CDB Microscopy Core Facility Perelman School of Medicine at the University of Pennsylvania 1107 BRB 2/3 421 Curie Boulevard Philadelphia, PA 19104
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Title-Subject: [Filtered] Reminder EGU 2020: Advances in microanalysis: Insights into nanoscale trace element heterogeneities
Message: Dear colleagues,
We would like to invite submissions of abstracts to our EGU 2020 session applying advanced microanalysis techniques to investigate chemical heterogeneities.
GMPV1.3 "Advances in microanalysis: Insights into nanoscale trace element heterogeneities" Convener: Renelle DubosqECS | Co-conveners: Tyler BlumECS, Sandra Piazolo
Invited speakers: Dr. Desmond Moser, Western University, will present on trace elements and microstructures from Early Mars and the geochronology of habitability
Dr. Ana Cernok, Royal Ontario Museum, will present on shock‐induced microtextures in lunar apatite and merrillite
Please follow this link: https://meetingorganizer.copernicus.org/EGU2020/session/35196 for a full description of the session.
Abstract submission (deadline: 15 January 2020, 13:00 CET)
Looking forward to seeing you in Vienna, Best regards,
Renelle, Tyler and Sandra
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Email: johnshields59-at-gmail.com Name: John Shields
Organization: University of Georgia
Title-Subject: [Filtered] Biological TEM Workshop, March 11
Message: *Biological TEM Workshop* This intensive, three-day workshop provides a practical and basic theoretical introduction to the Transmission Electron Microscope and biological sample preparation techniques. Each day will consist of lecture, discussion and *hands-on* training led by GEM staff. Who: Anyone requiring training on TEM and biological sample preparation. The workshop will be limited to 6 participants based on the availability of equipment. When: Wednesday through Friday, March 11-13 2020, 8am-5pm each day (lunch is provided) Where: 154 Barrow Hall, University of Georgia, Athens, GA 30602
Registration: Contact John Shields (jpshield-at-uga.edu) for more information and to sign up. Registration requires iLab account through the GEM website. https://uga.ilabsolutions.com/account/login Deadline: March 4, 2020
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Email: john.kron-at-atlanticexperts.com Name: John Kron
Organization: Atlantic Professional Services
Title-Subject: [Filtered] jeol JSM 6360 SEM
Message: Does anyone have any information on error codes, specifically error code 147 Vacuum system failure
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one of my customers is producing active carbon, I suppose, from different kind of wood.
Is there any (SEM microscopic) way to find out the active surface value in square meters per gram of carbon? In a more precise way than to estimate it?
Thanks, and happy new year
Stefan
--
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
My understanding is that the pore structure would push the limit of SEM to characterize it. I don't think there would be any reliable way to correlate the microstructure with an estimate of specific surface area. And if the operator or SEM was having an off day, they would fail to resolve the porosity and thus fail to properly compare samples.
I recall that BET is the norm for measuring specific surface. I presume it works for activated carbon. I think it would give much more reliable number than anything that could be done microscopically.
Warren Straszheim Materials Analysis and Research Lab Iowa State University
-----Original Message----- X-from: diller-at-stefan-diller.com {diller-at-stefan-diller.com} Sent: Wednesday, January 08, 2020 5:18 AM To: Straszheim, Warren E [BIOTC] {wesaia-at-iastate.edu}
Dear All,
one of my customers is producing active carbon, I suppose, from different kind of wood.
Is there any (SEM microscopic) way to find out the active surface value in square meters per gram of carbon? In a more precise way than to estimate it?
Thanks, and happy new year
Stefan
--
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Apologies for the double post, but the deadline for our advertised 3-year postdoc position in STEM imaging at Monash University has been extended to Friday 24th January. If you know anyone interested who may not have been able to apply over the holiday period, there's still time.
Full details at: http://careers.pageuppeople.com/513/cw/en/job/600527/research-fellow-in-atomicscale-structure-determination-in-thick-nanostructures
Kind regards, Scott
On 22/11/2019 8:08 am, Scott Findlay wrote: } Dear colleagues, } } I'm advertising a 3-year postdoc position in atomic-scale structure } determination in thick nanostructures at the School of Physics and } Astronomy, Monash University, Australia.  Further details below. } } Please bring this to the attention of anyone who may be interested. } } Many thanks, } Scott Findlay } } ___________________ } } Position Descriptions and application details at: } http://careers.pageuppeople.com/513/cw/en/job/600527/research-fellow-in-atomicscale-structure-determination-in-thick-nanostructures } } } Position overview: The Research Fellow will work on developing methods } for atomic-scale structure determination via scanning transmission } electron microscopy. This project aims to develop a theoretical and } computational toolkit for structure retrieval at atomic resolution that } is robust in the presence of multiple scattering (“dynamical } diffraction”) of the electron probe, and to apply it to large } experimental data sets obtained from the new generation of fast-readout } electron detectors. The project may draw on methods from inverse } scattering theory, phase retrieval, iterative algorithms, machine } learning, and high-performance computing. } } Duration: 3-year fixed-term appointment } Remuneration package: $68,040 - $92,343 pa Level A (plus 17% employer } superannuation) } Closing date: Friday 10th January, 2020
-- *SCOTT FINDLAY* Senior Lecturer
*Monash University* School of Physics and Astronomy G08, New Horizons Centre, 20 Research Way, Clayton Campus Clayton, VIC 3800 Australia
From normanatlas54ykur-at-gmail.com Wed Jan 8 16:53:37 2020 Return-Path: {normanatlas54ykur-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.162.215] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id 008Mrajs019679 for {microscopylistserverarchive7-at-microscopy.com} ; Wed, 8 Jan 2020 16:53:36 -0600 Received: from webmail.halftomorrow.com ([Thu, 09 Jan 2020 03:37:34 +0600]) by snmp.otwaloow.com with QMQP; Thu, 09 Jan 2020 03:37:34 +0600 Received: from unknown (HELO mxs.perenter.com) (Thu, 09 Jan 2020 03:26:46 +0600) by mail.webhostings4u.com with ASMTP; Thu, 09 Jan 2020 03:26:46 +0600 Received: from [162.44.252.169] by smtp18.yenddx.com with SMTP; Thu, 09 Jan 2020 03:24:53 +0600 Received: from unknown (38.247.92.211) by smtp4.cyberemailings.com with SMTP; Thu, 09 Jan 2020 03:11:34 +0600 Received: from rsmail.alkoholic.net ([126.90.161.200]) by nntp.pinxodet.net with QMQP; Thu, 09 Jan 2020 02:57:57 +0600 Message-ID: {64EC1C84.C2559439-at-gmail.com}
Hi Everyone,
We are in the midst of refurbishing an RMC MT-7000 ultramicrotome to replace our MT-7 teaching ultramicrotome that has developed a problem we haven't been able to fix. Unfortunately, the instrument I acquired is missing the horizontal overhead light (but luckily, not the mounting bar). RMC has been fantastic in support for this project, but doesn't have a light available that I can purchase. We've thought about modifying the light from the MT-7, but I still have hopes for getting it going again.
If anyone should happen to have an out of service MT-7000 and would be willing to part with the light fixture, curly cord and/or the plug for the light, please let me know. I have images of the overhead light available that I can send by regular email. The lamp from our other MT-7000 works perfectly with the unit under construction, so no worries about the rest of the circuit.
Thanks for any help, Heather
Heather A. Owen, Ph.D. Director, Electron Microscope Laboratory Department of Biological Sciences University of Wisconsin - Milwaukee 3209 N. Maryland Ave. Milwaukee, WI† 53211
Job Location: Johnson Matthey, Clean Air, Wayne, PA, USA
Job Description: The Development Analytical Services (DAS) Scientist II takes day-to-day responsibility for OM and SEM instrumentation and provides support to R&D projects that generate new products, processes and understanding of commercial value to JM while building an understanding of the science involved. The incumbent will perform routine analytical analyses using the scanning electron microscopy and a significant amount of non-routine work using existing analytical procedures that serve to characterize the properties, function, or composition of catalytic materials or materials of catalytic interest. The incumbent supports JM through all aspects of catalytical material discovery and delivery including analytical analysis, liaise with internal customers, and support of JM processes and practices. Individual will have responsibility for managing projects from inception to close.
For more details and how to apply: {https://chu.tbe.taleo.net/chu01/ats/careers/requisition.jsp?org=JOHNSONMATTHEY&cws=1&rid=9712} .
Louis Gambino, PhD Associate Scientist Johnson Matthey 436 Devon Park Drive Wayne, PA 19087-1816 If the reader of this email is not the intended recipient(s), please be advised that any dissemination, distribution or copying of this information is strictly prohibited. Johnson Matthey Inc. has its main place of business at 435 Devon Park Drive, Wayne, PA, 19087 USA. While Johnson Matthey aims to keep its network free from viruses you should note that we are unable to scan certain emails, particularly if any part is encrypted or password-protected, and accordingly you are strongly advised to check this email and any attachments for viruses. The company shall NOT ACCEPT any liability with regard to computer viruses transferred by way of email. Please note that your communication may be monitored in accordance with Johnson Matthey internal policy documentation.
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Email: chrisbrantner-at-gwu.edu Name: Chris Brantner
The Nanofabrication and Imaging Center at George Washington University will be holding its 4th annual CLEM workshop June 8-12, 2020 in Washington DC. If you are interested in applications and workflow for CLEM projects, then this workshop is for you.
Please visit the website for further details.
https://nic.gwu.edu/clem-workshop
Happy New Year Chris Brantner
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Title-Subject: [Filtered] Upcoming Microscopy Workshops at EMS Microscopy Academy
Message: Materials Ultramicrotomy February 24, 2020 For those unfamiliar with microtomy to prepare for the workshop. February 25 - 27, 2020 This workshop will introduce individuals to the unique application of ultramicrotomy to materials. Learn more at https://www.emsmicroscopyacademy.com/product-page/materials-feb20 Aurion Immunogold Silver Staining April 15 - 17, 2020 Three days of hands-on training for students, researchers, and microscopists who want to learn the most up to date theory and practice in immunogold labeling. Learn more at https://www.emsmicroscopyacademy.com/product-page/immunogold-apr20 Cryosectioning/Immunogold April 20 - 24, 2020 Hands-on training for students, researchers, and microscopists who want to learn the most up to date theory and practice in cryosectioning and immunogold labeling. Learn more at https://www.emsmicroscopyacademy.com/product-page/cryoimmuno-apr20
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The Analytical Instrumentation Facility (AIF) and Research Triangle Nanotechnology Network (RTNN) at North Carolina State University, in partnership with Protochips and ThermoFisher Scientific, are pleased to announce the first annual In Situ Microscopy Congress (ISMC). The meeting will be held at North Carolina State University in Raleigh, NC on March 2nd and 3rd, 2020.
This is a two day workshop which provides one the opportunity to learn about liquid phase and gaseous phase in situ capabilities, and includes two plenary talks from leaders in the respective fields along with hands-on demonstrations of liquid and gas cell holders and our Talos F200X, probe-corrected Titan, and Quanta 3D FIB/SEM platforms.
This workshop is open to all. Attendees are encouraged to submit an abstract to be considered for an oral or poster presentation. To register, or for more information, please visit the following registration link:
We look forward to seeing you, Chris -- Transmission Electron Microscopy Lab Manager Analytical Instrumentation Facility (AIF) NC State University https://www.aif.ncsu.edu/ Cell: 267-496-0587
==============================Original Headers============================== 6, 49 -- From crwinkler-at-ncsu.edu Mon Jan 13 18:14:24 2020 6, 49 -- Received: from mail-io1-f44.google.com (mail-io1-f44.google.com [209.85.166.44]) 6, 49 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 00E0EOJ0001110 6, 49 -- for {Microscopy-at-microscopy.com} ; Mon, 13 Jan 2020 18:14:24 -0600 6, 49 -- Received: by mail-io1-f44.google.com with SMTP id t26so11721675ioi.13 6, 49 -- for {Microscopy-at-microscopy.com} ; Mon, 13 Jan 2020 15:11:59 -0800 (PST) 6, 49 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 49 -- d=ncsu.edu; s=google; 6, 49 -- h=mime-version:from:date:message-id:subject:to; 6, 49 -- bh=jy/FXDxX1SDmv+M4BrhZrv6qZ1a/6WcuPc/gN88MZLA=; 6, 49 -- b=ZPgouZqxT/+9XFsRXj7H/A8k4ay8hTGSoIzfFnebjd7ZrcAIy163cU7wxl7NSw7WIl 6, 49 -- +LU7nk/pnnUWob+DUiGZYfnhceE9uiaABanwHMeNVJLuxKbX+atpooFiWjkIqQo20mRY 6, 49 -- N/39g5jv5qaJ3UBaLTH0DJzRXgacWYzGB+YikkEfkFnUPR5R1ZqTNktXbhJ8SbKzgxne 6, 49 -- SvtwRIE73/4RPbbpQxX8OQ7Ztex/dNq/XQWf1+PKozZY/rHrMq9swf8lyOmP2s4QQnRb 6, 49 -- UBWDvC5YAM8WqPEmTsZwOT2x/VQa8xjfftSpUhllquEja5NSQv4FANypZzkuPFQC0rEA 6, 49 -- xoig== 6, 49 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 49 -- d=1e100.net; s=20161025; 6, 49 -- h=x-gm-message-state:mime-version:from:date:message-id:subject:to; 6, 49 -- bh=jy/FXDxX1SDmv+M4BrhZrv6qZ1a/6WcuPc/gN88MZLA=; 6, 49 -- b=UJ+dTbjUKyk0DfLdawyri+9VtbSJ8QuZx9bw1nyUIJWzvzFFxanwaSh+ifue8weLxu 6, 49 -- hr2Ruz16gtPhDaIv/pH7/9Z8jYcBLyWGMH1dmwqPJXe5bnXMqVTTKcoRvFPk6884kA/9 6, 49 -- HSJYxei27cPCVbpfwFxpsAQ4ISZv/MmsVCWchxNhKgKOIRQN4YInNn0SIBjbEQ7BZXkm 6, 49 -- DbPIW4+Glneq7ZMeQLzIdcZSFzFlub0sfzl/tRznPuKDhEmIepXCa1KwvfNpfzdz6N/z 6, 49 -- Svc4KNFJ47euTBWxt/NpDlX8rOV7ihpmU0j8dsH571zkm866aOsLVwycSgXbZVL2cvQU 6, 49 -- RAew== 6, 49 -- X-Gm-Message-State: APjAAAX+XwLPPXkhCrmAs0p/CO940k/usRGyiGU4qlKBz+BZi3B0myZ+ 6, 49 -- kDtj4ob7Eyll0G2hNamE6FLrmcl0wS01uDhxkuzHtvO0JAw3i7YOtxMBxYB64U9FdgpuYZXBd/r 6, 49 -- BAWHTnthUPLXm9Mla66Sw1wp5Aj6kDwSU/tsp53LK6hOAhohX1VCit8sJzqt7iR1ny/me 6, 49 -- X-Google-Smtp-Source: APXvYqy1fW+j+9EYk0Hh3NwBpJoNwUw7zxiLU3a4dAHmGVaFgsZDY/KVHo8YZ1/aSxjQeWPBZXz26w== 6, 49 -- X-Received: by 2002:a02:742:: with SMTP id f63mr16442709jaf.138.1578957118637; 6, 49 -- Mon, 13 Jan 2020 15:11:58 -0800 (PST) 6, 49 -- Received: from mail-io1-f49.google.com (mail-io1-f49.google.com. [209.85.166.49]) 6, 49 -- by smtp.gmail.com with ESMTPSA id x77sm4290851ilk.34.2020.01.13.15.11.58 6, 49 -- for {Microscopy-at-microscopy.com} 6, 49 -- (version=TLS1_3 cipher=TLS_AES_128_GCM_SHA256 bits=128/128); 6, 49 -- Mon, 13 Jan 2020 15:11:58 -0800 (PST) 6, 49 -- Received: by mail-io1-f49.google.com with SMTP id n21so11708956ioo.10 6, 49 -- for {Microscopy-at-microscopy.com} ; Mon, 13 Jan 2020 15:11:58 -0800 (PST) 6, 49 -- X-Received: by 2002:a05:6638:24f:: with SMTP id w15mr16827781jaq.130.1578957117827; 6, 49 -- Mon, 13 Jan 2020 15:11:57 -0800 (PST) 6, 49 -- MIME-Version: 1.0 6, 49 -- From: Christopher Winkler {crwinkler-at-ncsu.edu} 6, 49 -- Date: Mon, 13 Jan 2020 18:11:46 -0500 6, 49 -- X-Gmail-Original-Message-ID: {CAA8T2PP83eLbLQ3kuosXxrNeFSymOCtd2-9u3HVUCXEKKKoSUQ-at-mail.gmail.com} 6, 49 -- Message-ID: {CAA8T2PP83eLbLQ3kuosXxrNeFSymOCtd2-9u3HVUCXEKKKoSUQ-at-mail.gmail.com} 6, 49 -- Subject: In Situ Microscopy Congress - NCSU AIF - March 2nd and 3rd 6, 49 -- To: Microscopy-at-microscopy.com 6, 49 -- Content-Type: text/plain; charset="UTF-8" ==============================End of - Headers==============================
From NegativeSEOyrfxu-at-mailinator.com Mon Jan 13 20:51:42 2020 Return-Path: {NegativeSEOyrfxu-at-mailinator.com} Received: from mailinator.com (hn.kd.ny.adsl [42.231.162.213] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id 00E2pfJH027377 for {microscopylistserverarchive7-at-microscopy.com} ; Mon, 13 Jan 2020 20:51:42 -0600 Message-ID: {151756C7.AAB54645-at-mailinator.com}
X-from: tescanmicroscopy-at-gmail.com
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Email: tescanmicroscopy-at-gmail.com Name: dj miller
Organization: Tescan USA
Title-Subject: [Filtered] position available - Applications Specialist in FIB-SEM Tescan USA
Message: TESCAN USA, a fast-growing solution provider in electron, ion and x-ray CT in the microscope business, is looking for an Applications Specialist in FIB-SEM. This position is responsible for supporting the sales process of Tescan FIB-SEMs through tool demonstrations, sample runs, development of new techniques, contributions to technical papers and other applications activities. Qualified candidates will have extensive experience in Scanning Electron and Ion Microscopy and intimate familiarity with FIB-SEM tools and techniques such as TEM sample Preparation, 3D cross-sectioning and reconstruction (image, EDS & EBSD), delayering, nano-probing, circuit edit, lithography and other. Experience in other techniques such as TOF-SIMS, cryo sample preparation, APT, TEM would be a plus. Excellent communication in English is required. This position located in the Pleasanton, CA, but will require travel up to 50% of the time.
TESCAN USA will only employ individuals who are legally authorized to work in the United States for this opening (i.e. ñ no sponsorship is available). Any offer of employment may be conditional upon the successful completion of a background investigation and drug screening. TESCAN USA offers competitive salaries and benefits. Qualified candidates should send their resume, cover letter and salary requirements to: Gary.Hawkinson-at-tescan.com.
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Email: georg.ramm-at-monash.edu Name: Georg Ramm
Organization: Monash Ramaciotti Centre Title-Subject: [Filtered] Staff position CLEM and Bio EM at Monash University, Melbourne, Australia
Message: We are looking for a staff member with expertise in CLEM to join our team at the Monash Ramaciotti Centre at Monash University (https://www.monash.edu/researchinfrastructure/cryo-em) Senior Research Officer - Correlative Light and Electron Microscopy Location: Clayton campus, Melbourne, Australia
Employment Type: Full-time, 3 year Fixed-term appointment
Contact: Georg Ramm, Georg.ramm-at-monash.edu, +61-3 - 9905 1280
Closing date: 31 January 2020 Link to job advertisement: https://careers.pageuppeople.com/513/cw/en/job/599948/senior-research-officer-correlative-light-and-electron-microscopy
As the successful candidate you will:
Manage, plan, coordinate and oversee EM projects in collaboration with users Develop new protocols for correlative light and electron microscopy and life sciences EM techniques and apply them to research projects Teach specialised EM techniques such as CLEM and immuno EM to Monash researchers and to the wider Australian and international EM community Perform microscopy and related EM techniques including immuno EM, (cryo-) ultramicrotomy, high pressure freezing, and cryo-preparation
The Monash Ramaciotti Centre is a leading facility for life sciences electron microscopy. It houses Australiaís first Titan Krios microscope, a cryo-FIBSEM Helios G4 with Leica VCT500 cryo-stage, a Talos Arctica, as well as two 120keV TEMs and a FESEM. A suite of advanced sample preparation and other equipment is available, including a Zeiss LSM900 Airyscan with Linkam cryo-stage, a Wohlwend high pressure freezer, Leica AFS2 and FC7 cryo-ultramicroscopes. The facilityís expert team supports and collaborates on a large number of bio EM techniques ranging from standard SEM and TEM to immuno EM, correlative light and electron microscopy, cryotomography and single particle analysis.
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==============================Original Headers============================== 14, 54 -- From microscopy.listserver-at-gmail.com Mon Jan 13 21:37:40 2020 14, 54 -- Received: from mail-il1-f182.google.com (mail-il1-f182.google.com [209.85.166.182]) 14, 54 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 00E3bege012610 14, 54 -- for {microscopy-at-microscopy.com} ; Mon, 13 Jan 2020 21:37:40 -0600 14, 54 -- Received: by mail-il1-f182.google.com with SMTP id s15so10132080iln.1 14, 54 -- for {microscopy-at-microscopy.com} ; Mon, 13 Jan 2020 18:35:15 -0800 (PST) 14, 54 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 14, 54 -- d=gmail.com; s=20161025; 14, 54 -- h=subject:references:to:from:message-id:date:user-agent:mime-version 14, 54 -- :in-reply-to:content-language:content-transfer-encoding; 14, 54 -- bh=Sn2y0f89Mh4r8oYttlALvoBIzs0SKEeNaQvfe58RP5s=; 14, 54 -- b=sxGh3u07OFI3bgsaUEu8jwzkQYmZq0BFhDcm3hGgNRrdfaBXJw6QNgm5dcitDJlZU0 14, 54 -- WqBy1rEBsYYmwuhrZBNmTWLYIBss5GlbwMq1+/WbNvlIAiNsnOjAkwyMqU+gbQqPk99D 14, 54 -- pUtJulK3Eg+I9Bq6JLbRBlOhnUptKCB/xjnEGLbzwfV1Ml1yAkIA1cWcQa5BPy3CgvI2 14, 54 -- aK+Ff8osk/KES6o+MS9NgAuC5D1WQEQ38cP5d7g0Oov017TfsntCtAQKdST3PLQHNBSY 14, 54 -- BAa4XIMxjzEe30DWLR144C4aTdovW+2MVE41wrnVXT5K5OV8lihenYc9cYBuN7F7HFkt 14, 54 -- 1v+g== 14, 54 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 14, 54 -- d=1e100.net; s=20161025; 14, 54 -- h=x-gm-message-state:subject:references:to:from:message-id:date 14, 54 -- :user-agent:mime-version:in-reply-to:content-language 14, 54 -- :content-transfer-encoding; 14, 54 -- bh=Sn2y0f89Mh4r8oYttlALvoBIzs0SKEeNaQvfe58RP5s=; 14, 54 -- b=gdhM5F7B3vaEextjM8o9G5t4w9TCzoc7jDFFKBCTiItXavneUrKnKyWX3xB/rh7AvB 14, 54 -- 71umfj7Ffihi9fEVILpnPoTS3bkDBlbkGoNDejyPdzwBT7RrKSysuNpWWOhFwxaej0B6 14, 54 -- WnUKcaPJ+SuKMW/xhnyMwzaN0RprAFaYmXw3jxiprDyOngSa2Noi+hJLKz9fdBFA6z/0 14, 54 -- HPGlRoF800tv75WRGuZl2ncek0mSAG1AETKMM4x8VCGfq1tGpwGuolFl4ArFJoJ/VKg/ 14, 54 -- kxVoXaFSgbad4Pv+C2h1/POq1HbsDvW9ru6h62r4B4mtzEv5E3E3i/Bj+zhKbntxXH+Q 14, 54 -- 1P3A== 14, 54 -- X-Gm-Message-State: APjAAAVLEY6ZBiEbkZik1JCpoPmFel1Hm6ajRSBG3i4zM9PGSVLvzltA 14, 54 -- jtwBZkQfRvv0KSfWVFKnCPYPI5nX 14, 54 -- X-Google-Smtp-Source: APXvYqyhg4DcHTpPfxL4P5/4ZL6zXe+l6G7F00tgH9170dcUXCH14LM1g/lcCoyAOGuGzh1AurIhsA== 14, 54 -- X-Received: by 2002:a92:7787:: with SMTP id s129mr1421441ilc.129.1578969314818; 14, 54 -- Mon, 13 Jan 2020 18:35:14 -0800 (PST) 14, 54 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:4432:db4b:adc8:d84b]) 14, 54 -- by smtp.googlemail.com with ESMTPSA id d12sm4485981iln.63.2020.01.13.18.35.14 14, 54 -- for {microscopy-at-microscopy.com} 14, 54 -- (version=TLS1_3 cipher=TLS_AES_128_GCM_SHA256 bits=128/128); 14, 54 -- Mon, 13 Jan 2020 18:35:14 -0800 (PST) 14, 54 -- Subject: viaWWW:Staff position CLEM and Bio EM at Monash University, 14, 54 -- Melbourne, Australia 14, 54 -- References: {202001130304.00D34SuG007875-at-microscopy.com} 14, 54 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 54 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} 14, 54 -- X-Forwarded-Message-Id: {202001130304.00D34SuG007875-at-microscopy.com} 14, 54 -- Message-ID: {1a370250-39d1-95c5-4302-389e8e1379b9-at-gmail.com} 14, 54 -- Date: Mon, 13 Jan 2020 20:35:14 -0600 14, 54 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.14; rv:68.0) 14, 54 -- Gecko/20100101 Thunderbird/68.3.1 14, 54 -- MIME-Version: 1.0 14, 54 -- In-Reply-To: {202001130304.00D34SuG007875-at-microscopy.com} 14, 54 -- Content-Type: text/plain; charset=windows-1252; format=flowed 14, 54 -- Content-Language: en-US 14, 54 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
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Email: bowmanpond-at-gmail.com Name: Mike Codner
Title-Subject: [Filtered] Light Leakage from Microscope into Digital Camera
Message: While familiarizing myself with a newly purchased Swiftcam 18 MP Microscope Camera, I was having a problem mating it to my LW Scientific Revelation III Binocular Microscope using Swift's 3.0 software and Windows 10. Upon viewing pond water specimens, the background lighting kept changing from white to darker colors and vice-versa. I had made all the adjustments the manual had recommended including exposure, white balance, color settings, and the like. Nothing worked. To make a long story short, the problem resolved itself when I plugged the non-camera binocular with a black rubber stopper after removing the lens. Apparently there had been light leakage from this binocular into the one containing the camera. Just wanted to alert your members since this problem was very frustrating and I could find no helpful information either in the manual or online.
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Email: sichen-at-anl.gov
Name: Si Chen
Organization: Argonne National Laboratory
Title-Subject: [Filtered] Post Doctoral Position - Developing a Cryo-compatible Specimen Prep workflow for Xray and Electron Microscopy -at- Argonne National Laboratory
Message: Position Description
The X-ray Science Division (XSD) of Advanced Photon Source (APS) at Argonne National Laboratory is looking for a Postdoctoral Researcher who will be responsible for developing novel apparatus and methods to enable multi-scale and multi-modality analysis of materials via both electron microscopy and X-ray microscopy at the synchrotron facility. The research aims to deliver a cryo-compatible workflow and supporting techniques to optimize sample preparation for X-ray fluorescence nanoprobe as well as the combined use of multiple analytical platforms. It will also involve software development for image registration across different analytical platforms and correlative data analysis. The successful candidate will work in a multidisciplinary team environment including physicists, chemists, biologists and computer scientist. The candidate will lead the effort in developing the methodology, the deployment at the APS beamlines, as well as applications to materials, biological and environmental sciences. Results will be reported in appropriate forms: publications in refereed journals and oral presentations at meetings, conferences, and seminars. The position will begin in April 2020. It is a one-year position, and renewable for a second year.
Position Requirements
*Ph.D. degree in physics, materials science, engineering, or a related discipline; *Comprehensive knowledge in at least two of the following fields: X-ray physics, X-ray microscopy, electron microscopy, image processing; *Considerable experience with electron microscopy, focused ion beam, synchrotron facility, preferably cryogenic instruments; *Strong data analysis and trouble-shooting skills; *Considerable experience with programming and software tools such as python and git; *Experience with automation, control, and instrument infrastructure is a plus; *Knowledge of biology, chemistry is a plus; *Experience with cryogenic is a plus; *Demonstrated experience working successfully as part of a team in a collaborative and multidisciplinary scientific environment; *Ability to communicate effectively with internal and external collaborators and ability to work in a team environment.
Additional Details and Application Forms can be found at this URL
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Email: whiteto-at-missouri.edu Name: Tommi White
Organization: University of Missouri Electron Microscopy Core
Title-Subject: [Filtered] Cellular & Molecular Electron Microscopy
Message: Hello Microscopy Listserve,
I am taking over a graduate level class entitled "Cellular & Molecular Electron Microscopy" from a retiring faculty member. Would any of you be so kind to share syllabus/lecture materials that I could use in this course? Of course, full credit will be given to the provider of the information! :) Thanks in advance to helping educate the next generation of electron microscopists and light that fire. Tommi Tommi A. White, Ph.D. Director, Electron Microscopy Core Assistant Research Professor, Biochemistry University of Missouri Mail: W117 Veterinary Medicine Bldg 1520 E Rollins St., Columbia, MO 65211 EMC: 573-882-8304 Direct: 573-884-7338 Email: whiteto-at-missouri.edu Web: http://emc.missouri.edu Tweets: -at-MizzouEMC
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There is still plenty of time to apply for Analytical and Quantitative Light Microscopy 2020, held at the Marine Biological Laboratory, Woods Hole, MA, USA. The course will run April 28-May 8, 2020.
Applications are due February 5, 2020. Apply through the course web site at http://www.mbl.edu/aqlm. We especially invite those from underrepresented groups to apply. Reach out if you have any questions!
AQLM is a comprehensive and intensive course in light microscopy for researchers in biology, medicine, and material sciences. This course provides a systematic and in-depth examination of the theory of image formation and application of digital methods for exploring subtle interactions between light and the specimen. This course emphasizes the quantitative issues that are critical to the proper interpretation of images obtained with modern wide-field microscopes, confocal microscopes, and new emerging technologies.
Applications are due February 5, 2020. Apply through the course web site at http://www.mbl.edu/aqlm.
Some refer to it as "microscopy boot camp" because we work hard, but we have a lot of fun in the process!
Course Directors: Peter Kner, University of Georgia Paul Maddox, University of North Carolina at Chapel Hill Wendy Salmon, Whitehead Institute for Biomedical Research Course Laboratory Director: Gary Laevsky, Princeton University
Happy Imaging! Peter, Paul, Wendy and Gary
Best, Wendy
-------------------- Wendy C Salmon, M.A. Light Microscopy Specialist W.M. Keck Facility for Biological Imaging Whitehead Institute for Biomedical Research 455 Main St., Rm 447 Cambridge, MA 02142 e: wsalmon-at-wi.mit.edu w: microscopy.wi.mit.edu
The CCEM just announced that it will be holding its annual summer school on electron microscopy from June 8 - 12, 2020!
A 5-DAY COURSE for users with experience in electron microscopy, on the fundamentals of aberration-corrected imaging, electron energy loss spectroscopy, energy dispersive X-ray spectroscopy, electron tomography, ultimate physical limits (beam damage and resolution), DPC microscopy and the use of aberration-corrected electron microscopes. The aim is to provide students a device in solving characterization problems with the help of experts. The course will include lectures given by experts in the use of the technique and experts in electron optics, alignment and optimization of electron microscopes and EELS spectrometers. Students will have plenty of opportunities for hands-on training on the alignment and operation of the electron microscopes with the experts from the microscope and spectrometer companies. Two FEI Titan microscopes with correctors and monochromators (GIF Quantum and K2) will be used for training, as well as a Talos F200X. Several hands-on data processing sessions are also organised.
DATE: June 8 - 12, 2020
COST: All meals and course notes are included in the registration fee ranging from $700.CDN/full-time students to $2000.CDN/industry researchers. Accommodation will be separate and the responsibility of attendees (see full details on registration form).
REGISTRATION: Register online by January 31, 2020: https://ccem.mcmaster.ca/ccem-summer-school-2020
--† Dr. Andreas Korinek
Manager
Canadian Centre for Electron Microscopy McMaster University 1280 Main Street West, Hamilton ON Canada, L8S 4M1 phone: +1 905-525-9140 ext 20400
==============================Original Headers============================== 10, 24 -- From korinek-at-mcmaster.ca Tue Jan 21 16:06:23 2020 10, 24 -- Received: from FHSHC4H16-1.csu.mcmaster.ca (fhshc4h16-1.csu.mcmaster.ca [130.113.22.3]) 10, 24 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 00LM6NCV011274 10, 24 -- for {microscopy-at-microscopy.com} ; Tue, 21 Jan 2020 16:06:23 -0600 10, 24 -- Received: from FHSDB4H16-2.csu.mcmaster.ca ([fe80::74a1:2461:9f81:dcf6]) by 10, 24 -- FHSHC4H16-1.csu.mcmaster.ca ([2002:8271:1603::8271:1603]) with mapi id 10, 24 -- 14.03.0468.000; Tue, 21 Jan 2020 16:04:23 -0500 10, 24 -- From: "Korinek, Andreas" {korinek-at-mcmaster.ca} 10, 24 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 10, 24 -- Subject: CCEM TEM Summer School 2020 10, 24 -- Thread-Topic: CCEM TEM Summer School 2020 10, 24 -- Thread-Index: AdXQni4YUMlvldb5RU+8pIUNlcb1PQ== 10, 24 -- Date: Tue, 21 Jan 2020 21:04:23 +0000 10, 24 -- Message-ID: {4CB3BB07DE33A340B9F8F8DAFE73D7A50128A63437-at-FHSDB4H16-2.csu.mcmaster.ca} 10, 24 -- Accept-Language: en-CA, en-US 10, 24 -- Content-Language: en-US 10, 24 -- X-MS-Has-Attach: 10, 24 -- X-MS-TNEF-Correlator: 10, 24 -- x-originating-ip: [130.113.22.232] 10, 24 -- x-tm-snts-smtp: 1EFC72829D7B8CA3BCC17918393EA6E5DF4299295DDE40C98E96DACBA1D4A6742 10, 24 -- Content-Type: text/plain; charset="iso-8859-1" 10, 24 -- MIME-Version: 1.0 10, 24 -- Content-Transfer-Encoding: 8bit 10, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id 00LM6NCV011274 ==============================End of - Headers==============================
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Email: bassimn-at-mcmaster.ca
Name: Nabil Bassim
Organization: McMaster University
Title-Subject: [Filtered] Postdoc/Research Associate Position Available - McMaster University
Message: The Bassim research group is seeking a postdoc or research associate who will work on a varied portfolio of projects, including developing novel 2-D van der Waals heterostructures, catalysts, and metamaterials. The chosen candidate would also be encouraged to develop their own microscopy techniques using the CCEM infrastructure. The research projects involve strong collaboration with synthesis teams, and proven ability to work in interdisciplinary groups should be demonstrated. Teaching, communication, and presentation skills are part of a successful post-doctoral or research associate position, and academic/industrial/governmental experience in these areas should be clearly detailed. Day-to-day supervisory tasks with graduate students in the CCEM and within the Bassim research group would be expected, and examples of administrative, project management, and supervisory skills would be a plus. Requirements for the role include: MANDATORY - A Ph.D. in Materials Science or Physics - Experience in aberration-corrected imaging and electron energy loss spectroscopy - A strong track record in publishing TEM-related research
DESIRED - Interest in mentoring graduate students - Experience with in-situ techniques - Capability to perform image simulations and scattering theory - Sample preparation capabilities using FIB Pay will range based on level of experience and track record. The appointment is for 1 year, renewable up to 3 years.
Located at McMaster University in Hamilton, Ontario, the work would be performed at the Canadian Centre for Electron Microscopy (CCEM), which is home to 10 technical staff and hundreds of users with many diverse interests. The CCEM has a very elaborate suite of advanced instrumentation with plans for future expansion. Hamilton is a lovely city in Southern Ontario, located midway between Toronto and Niagara Falls with a mild (by Canadian standards) winter.
If you are interested, please contact bassimn at mcmaster.ca with a CV and several references.
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Message: We are looking to extend the life of our WinNT XL30 ESEM with an upgrade. It seems FEI no longer offers an upgrade but there are apparently several options available through third party vendors.
I would appreciate hearing directly from anyone willing to share their experiences who have gone the third party route.
Also if you're contemplating decommissioning a Win 2000 XL30 in the near future and are looking for someone to take it off your hands, please contact me.
Thanks, Bill Mushock wim5-at-lehigh.edu
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----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
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Email: xbren-at-uw.edu Name: Shirley
Organization: UW
Title-Subject: [Filtered] UCT controller Message: Hi, Can someone give me any ideas? One of my lab's ultra-cut microtome(Leica UCT) controller doesn't work any more, and we found that the power unit is down. We reached Leica, but they said they don't have this model and don't do repair on this model. The microtome is good, but it's only the controller that doesn't work. Do you know where to repair/replace this kind of controller?
Many thanks!
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Email: jhyun-at-gatan.com Name: John Hyun
Organization: Gatan, Inc.
Title-Subject: [Filtered] EELS & EFTEM Analysis Training School April 2020
Message: EELS & EFTEM Analysis Training School April 2020 April 21 ñ 24, 2020 Gatan R&D Headquarters, Pleasanton, CA
We invite you to our upcoming course to learn best practices to set up your EELS hardware, optimize experimental protocols, then capture, and extract the maximum amount of compositional and chemical information from your TEM samples. Topics include:
ï Fundamentals of EELS and energy-filtered imaging in TEM ï Principles of operation of EFTEM and EELS systems ï Optimization of EFTEM and EELS data acquisition ï Quantification of elemental composition ï Other information provided by EFTEM/EELS and how best to extract it ï Use of EELS signals to form maps of elemental and chemical composition ï EFTEM and STEM EELS spectrum imaging techniques ï Identification of material phases via EELS fine structure mapping ï Applications to biological and physical science specimens
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Title-Subject: [Filtered] pathological report for mouse kidney EM
Message: Hi, Do you know any lab that could do TEM of mouse kidneys, including a pathology report?
Closer to NYC area would be a plus.
Thank you!
AMALIA Hilda Amalia Pasolli, Ph.D. Director and Research Associate Professor Electron Microscopy Resource Center RRB 120F The Rockefeller University 1230 York Avenue, Box 230 New York, NY 10065 Phone 212 327 8325
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Message: Location: Peter MacCallum Cancer Centre, Melbourne Australia
Peter MacCallum Cancer Centre is seeking an experienced and highly motivated scientist to join the Centre for Advanced Histology and Microscopy (CAHM). CAHM encompasses research histology, optical and electron microscopy and provides researchers with advice, tuition and technical expertise.
The applicant will have a passion for optical microscopy and in-depth understanding of the operation of optical microscopes, including confocal, super resolution, and multiphoton microscopy. The applicant will enjoy interacting with researchers and assisting them with their imaging needs.
The position is initially for a fixed term of 12 months with the opportunity for future extension.
Contact Person: Sarah Ellis Contact Number: +613 8559 7822 Contact Email: sarah.ellis-at-petermac.org Closing Date: 08 April 2020
To see the position description and apply go to https://petermac.mercury.com.au/ViewPosition.aspx?id=heS2osrVG/U=&jbc=ere
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X-from: Lee Cohen-Gould {lcgould-at-med.cornell.edu}
Hi Amalia- Happy New Year. What kind of pathology do you expect?† In the cortex or medulla?† Glomeruli, tubules or calex? I can send you a protocol for mouse kidney, or we can do it here, but we don't do the pathology analysis.† You should speak to the people in your animal facility. They should be able to either do it or refer you to someone, perhaps at the Animal Medical Center. I could put you in touch with our clinical EM facility here. They specialize in human kidney pathology. Let me know what you need. Best, Lee
---------------------------------------------------------------------------------------------------- *From:* microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} *Sent:* Wednesday, January 29, 2020 10:14 AM *To:* Lee Cohen-Gould {lcgould-at-med.cornell.edu} *Subject:* [EXTERNAL] [Microscopy] viaWWW: pathological report for mouse kidney EM
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Title-Subject: [Filtered] pathological report for mouse kidney EM
Message: Hi, Do you know any lab that could do TEM of mouse kidneys, including a pathology report?
Closer to NYC area would be a plus.
Thank you!
AMALIA Hilda Amalia Pasolli, Ph.D. Director and Research Associate Professor Electron Microscopy Resource Center RRB 120F The Rockefeller University 1230 York Avenue, Box 230 New York, NY 10065 Phone 212 327 8325
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Email: harry.murray-at-dfo-mpo.gc.ca
Name: Harry M. Murray
Organization: Fisheries and Oceans Canada
Title-Subject: [Filtered] Error Code for SU1510
Message: We purchased a Hitachi SU1510 SEM about 4 years ago and have had some good luck with it but recently our roughing pump has gone down and while now functionalÖ.we still canít get our scope to boot up. We keep getting the 7313 error code indicating a pump issue still exists. We are wondering if anybody might have experienced such an error in the past and thus might know a fix that would not require a Hitachi engineer and the associated cost.
Thanks in advance,
Harry
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Title-Subject: [Filtered] M&M 2020 - Important Submission Info!
Message: The M&M 2020 submission portal is OPEN. The submission DEADLINE is February 21, 2020; 11:59 PM U.S. Pacific Time. Click on the link below to access the online portal: {b} https://www.abstractscorecard.com/cfp/submit/login.asp?EventKey=AKMWPRYI {b}
Please note! M&M is using a NEW submission system this year, so the form structure and process are different from recent previous years. Visit the M&M 2020 website for important details and submission information! https://www.microscopy.org/MandM/2020/program/submit.cfm
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Organization: Royal Microscopical Society
Title-Subject: [Filtered] European Microscopy Congress 2020 - Abstract Submission Deadline 1 March 2020
Message: The European Microscopy Congress 2020 (emc2020) in Copenhagen is being hosted by SCANDEM, and organised by the Royal Microscopical Society under the auspices of the EMS and IFSM. The European Microscopy Congress is known for being the largest European stage for cross-disciplinary research. The broad scientific programme will welcome contributions from all over the world, showcasing the latest research in life sciences, physical sciences and engineering across all microscopy and imaging techniques. Over 30 conference sessions, an exhibition with more than 100 companies represented and a brilliant selection of features such as pre-event workshops and social networking events.
Oral and Poster abstract submission is currently open for the European Microscopy Congress 2020. Go to www.emc2020.eu/abstract-submission where you can create an account and then submit your abstract. The deadline for submitting your abstract is 1 MARCH 2020.
We are accepting abstracts for the following symposia: ï Life Sciences: Applications ï Life Sciences: Tools & Techniques ï Physical Sciences: Applications ï Physical Sciences: Tools & Techniques ï Data Handling and Analysis
To see a full list of the individual sessions and their descriptions, the provisional programme including the fantastic line up of invited speakers, please visit: https://www.emc2020.eu/conference/conference-sessions.html. You can also view the confirmed plenary speakers here: https://www.emc2020.eu/conference/plenary-speakers.html.
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Electron source with beam current of a few uA wouldn't exactly be from SEM or EBL domain, but.... I would suggest to try speaking to: Kimball Physics https://www.kimballphysics.com/
No connection other then a satisfied customer here.
Valery
Valery Ray (also with REFINE Lab, UCONN) ========================================== MEO Engineering Company, Inc. DBA "PBS&T" 290 Broadway, Suite 298, Methuen MA 01844 www.partbeamsystech.com www.freudlabs.com E-mail: vray-at-partbeamsystech.com Phone: +1-978-305-0479
On 2/6/2020 5:25 AM, koeck-at-kth.se wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } ​ } } I'm looking for an electron source, used or new, that can produce a beam with the following characteristics: } } energy: 1 keV or less } current: in the order of a few microAmps } beam waist diameter: 1 micrometer or less } } I'm thinking of a gun and a single condenser lens if that is possible. Small is good. } } Something used in a SEM, in e-beam lithography or electron evaporation might be a possibility. } } Can anyone recommend something? } } All the best, } } Philip } } } ==============================Original Headers============================== } 9, 44 -- From koeck-at-kth.se Thu Feb 6 04:24:56 2020 } 9, 44 -- Received: from smtp-3.sys.kth.se (smtp-3.sys.kth.se [130.237.48.192]) } 9, 44 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 016AOt2x016207 } 9, 44 -- for {microscopy-at-microscopy.com} ; Thu, 6 Feb 2020 04:24:56 -0600 } 9, 44 -- Received: from smtp-3.sys.kth.se (localhost.localdomain [127.0.0.1]) } 9, 44 -- by smtp-3.sys.kth.se (Postfix) with ESMTP id 70CA4A197 } 9, 44 -- for {microscopy-at-microscopy.com} ; Thu, 6 Feb 2020 10:21:32 +0100 (CET) } 9, 44 -- X-Virus-Scanned: by amavisd-new at kth.se } 9, 44 -- Received: from smtp-3.sys.kth.se ([127.0.0.1]) } 9, 44 -- by smtp-3.sys.kth.se (smtp-3.sys.kth.se [127.0.0.1]) (amavisd-new, port 10024) } 9, 44 -- with LMTP id Ls36YmGqaqdO for {microscopy-at-microscopy.com} ; } 9, 44 -- Thu, 6 Feb 2020 10:21:31 +0100 (CET) } 9, 44 -- Received: from exdb02.ug.kth.se (exdb02.ug.kth.se [192.168.32.112]) } 9, 44 -- by smtp-3.sys.kth.se (Postfix) with ESMTPS id BE9BEA0CD } 9, 44 -- for {microscopy-at-microscopy.com} ; Thu, 6 Feb 2020 10:21:31 +0100 (CET) } 9, 44 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=kth.se; s=default; } 9, 44 -- t=1580980891; bh=Wy1rFUq+dDYbR/0UIILMHN9U2veLzq4h3W6GL8RulTA=; } 9, 44 -- h=From:To:Subject:Date; } 9, 44 -- b=XB6XzMXCuDFNZXxVSisrZJ/j8aThW2472gE9kX9OxpPEdAGFzldnLl9P7+OR3LVV/ } 9, 44 -- QTFX3Qxbqaez1ZgIYOPAC7c5/rd2/IFF7vGNUu491N4NPfpMiNTSQXezh/ANjXp2hd } 9, 44 -- vHtSMvVoBEwhPe3hP34MpsPL0vfvJ9qGgv1R0Z0Y= } 9, 44 -- Received: from exdb01.ug.kth.se (192.168.32.111) by exdb02.ug.kth.se } 9, 44 -- (192.168.32.112) with Microsoft SMTP Server (TLS) id 15.0.1473.3; Thu, 6 Feb } 9, 44 -- 2020 10:21:31 +0100 } 9, 44 -- Received: from exdb01.ug.kth.se ([192.168.32.111]) by exdb01.ug.kth.se } 9, 44 -- ([192.168.32.111]) with mapi id 15.00.1473.005; Thu, 6 Feb 2020 10:21:30 } 9, 44 -- +0100 } 9, 44 -- From: =?utf-8?B?UGhpbGlwIEvDtmNr?= {koeck-at-kth.se} } 9, 44 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} } 9, 44 -- Subject: electron source } 9, 44 -- Thread-Topic: electron source } 9, 44 -- Thread-Index: AQHV3M3i8bnQRRu9D0Sdsm6FG8iYOw== } 9, 44 -- Date: Thu, 6 Feb 2020 09:21:30 +0000 } 9, 44 -- Message-ID: {1580980891958.41450-at-kth.se} } 9, 44 -- Accept-Language: sv-SE, en-US } 9, 44 -- Content-Language: sv-SE } 9, 44 -- X-MS-Has-Attach: } 9, 44 -- X-MS-TNEF-Correlator: } 9, 44 -- x-ms-exchange-transport-fromentityheader: Hosted } 9, 44 -- x-originating-ip: [46.59.23.142] } 9, 44 -- Content-Type: text/plain; charset="utf-8" } 9, 44 -- MIME-Version: 1.0 } 9, 44 -- Content-Transfer-Encoding: 8bit } 9, 44 -- X-MIME-Autoconverted: from base64 to 8bit by microscopy.com id 016AOt2x016207 } ==============================End of - Headers============================== }
Electron source with beam current of a few uA wouldn't exactly be from SEM or EBL domain, but.... I would suggest to try speaking to: Kimball Physics https://www.kimballphysics.com/
No connection other then a satisfied customer here.
Valery
Valery Ray (also with REFINE Lab, UCONN) ========================================== MEO Engineering Company, Inc. DBA "PBS&T" 290 Broadway, Suite 298, Methuen MA 01844 www.partbeamsystech.com www.freudlabs.com E-mail: vray-at-partbeamsystech.com Phone: +1-978-305-0479
On 2/6/2020 5:25 AM, koeck-at-kth.se wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } ​ } } I'm looking for an electron source, used or new, that can produce a beam with the following characteristics: } } energy: 1 keV or less } current: in the order of a few microAmps } beam waist diameter: 1 micrometer or less } } I'm thinking of a gun and a single condenser lens if that is possible. Small is good. } } Something used in a SEM, in e-beam lithography or electron evaporation might be a possibility. } } Can anyone recommend something? } } All the best, } } Philip } } } ==============================Original Headers============================== } 9, 44 -- From koeck-at-kth.se Thu Feb 6 04:24:56 2020 } 9, 44 -- Received: from smtp-3.sys.kth.se (smtp-3.sys.kth.se [130.237.48.192]) } 9, 44 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 016AOt2x016207 } 9, 44 -- for {microscopy-at-microscopy.com} ; Thu, 6 Feb 2020 04:24:56 -0600 } 9, 44 -- Received: from smtp-3.sys.kth.se (localhost.localdomain [127.0.0.1]) } 9, 44 -- by smtp-3.sys.kth.se (Postfix) with ESMTP id 70CA4A197 } 9, 44 -- for {microscopy-at-microscopy.com} ; Thu, 6 Feb 2020 10:21:32 +0100 (CET) } 9, 44 -- X-Virus-Scanned: by amavisd-new at kth.se } 9, 44 -- Received: from smtp-3.sys.kth.se ([127.0.0.1]) } 9, 44 -- by smtp-3.sys.kth.se (smtp-3.sys.kth.se [127.0.0.1]) (amavisd-new, port 10024) } 9, 44 -- with LMTP id Ls36YmGqaqdO for {microscopy-at-microscopy.com} ; } 9, 44 -- Thu, 6 Feb 2020 10:21:31 +0100 (CET) } 9, 44 -- Received: from exdb02.ug.kth.se (exdb02.ug.kth.se [192.168.32.112]) } 9, 44 -- by smtp-3.sys.kth.se (Postfix) with ESMTPS id BE9BEA0CD } 9, 44 -- for {microscopy-at-microscopy.com} ; Thu, 6 Feb 2020 10:21:31 +0100 (CET) } 9, 44 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=kth.se; s=default; } 9, 44 -- t=1580980891; bh=Wy1rFUq+dDYbR/0UIILMHN9U2veLzq4h3W6GL8RulTA=; } 9, 44 -- h=From:To:Subject:Date; } 9, 44 -- b=XB6XzMXCuDFNZXxVSisrZJ/j8aThW2472gE9kX9OxpPEdAGFzldnLl9P7+OR3LVV/ } 9, 44 -- QTFX3Qxbqaez1ZgIYOPAC7c5/rd2/IFF7vGNUu491N4NPfpMiNTSQXezh/ANjXp2hd } 9, 44 -- vHtSMvVoBEwhPe3hP34MpsPL0vfvJ9qGgv1R0Z0Y= } 9, 44 -- Received: from exdb01.ug.kth.se (192.168.32.111) by exdb02.ug.kth.se } 9, 44 -- (192.168.32.112) with Microsoft SMTP Server (TLS) id 15.0.1473.3; Thu, 6 Feb } 9, 44 -- 2020 10:21:31 +0100 } 9, 44 -- Received: from exdb01.ug.kth.se ([192.168.32.111]) by exdb01.ug.kth.se } 9, 44 -- ([192.168.32.111]) with mapi id 15.00.1473.005; Thu, 6 Feb 2020 10:21:30 } 9, 44 -- +0100 } 9, 44 -- From: =?utf-8?B?UGhpbGlwIEvDtmNr?= {koeck-at-kth.se} } 9, 44 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} } 9, 44 -- Subject: electron source } 9, 44 -- Thread-Topic: electron source } 9, 44 -- Thread-Index: AQHV3M3i8bnQRRu9D0Sdsm6FG8iYOw== } 9, 44 -- Date: Thu, 6 Feb 2020 09:21:30 +0000 } 9, 44 -- Message-ID: {1580980891958.41450-at-kth.se} } 9, 44 -- Accept-Language: sv-SE, en-US } 9, 44 -- Content-Language: sv-SE } 9, 44 -- X-MS-Has-Attach: } 9, 44 -- X-MS-TNEF-Correlator: } 9, 44 -- x-ms-exchange-transport-fromentityheader: Hosted } 9, 44 -- x-originating-ip: [46.59.23.142] } 9, 44 -- Content-Type: text/plain; charset="utf-8" } 9, 44 -- MIME-Version: 1.0 } 9, 44 -- Content-Transfer-Encoding: 8bit } 9, 44 -- X-MIME-Autoconverted: from base64 to 8bit by microscopy.com id 016AOt2x016207 } ==============================End of - Headers============================== }
*********************************************************************************** Forwarded from "Ask a Microscopist" Please remember that the person asking the question is likely not a member the listserver, and **any reply should go directly to the poster** as well as to the list. Using the "reply" function in your email does *not* send your answer to the person asking the question. Please copy their email address from their question. *********************************************************************************** Name: Aubrey Penn Email: anpenn-at-ncsu.edu
Avoiding surface damage during FIB sample preparation is fairly straight-forward: prior to ever exposing sample ion beam it should be coated with sufficient thickness (~100nm optimal, but } 30nm is a minimal requirement) of some protective layer that will "absorb" ion beam damage.
Following coatings may typically be used for such protective layer, depending on nature of the sample: (a) e-beam deposition of C, Pt, Mo, W, SiOx, etc...; (b) evaporated carbon or metal; (c) sputter coating by Au, Au/Pd, TiO, Cr, Ir, etc...; (d) conductive polymer coating by spinning or ultrasonic nozzle dispensing; (e) ink coating (i.e. "Sharpie trick), etc...
For atomic resolution TEM amorphous layer on the sides of the lamella would also need to be cleaned, but that is already another question.
Happy sample prepping :) Valery
Valery Ray (also with REFINE Lab, UCONN) ========================================== MEO Engineering Company, Inc. DBA "PBS&T" 290 Broadway, Suite 298, Methuen MA 01844 www.partbeamsystech.com www.freudlabs.com E-mail: vray-at-partbeamsystech.com Phone: +1-978-305-0479
On 2/7/2020 11:52 AM, oshel1pe-at-cmich.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } *********************************************************************************** } Forwarded from "Ask a Microscopist" } Please remember that the person asking the question is likely not a member the listserver, and } **any reply should go directly to the poster** as well as to the list. } Using the "reply" function in your email does *not* send your answer to the person asking the question. } Please copy their email address from their question. } *********************************************************************************** } Name: Aubrey Penn } Email: anpenn-at-ncsu.edu } Subject: FIB sample preparation } Your Question: What are the best ways to avoid damaging or outright destroying an epitaxial thin film (~20nm thick) film using Ga ion FIB for atomic resolution STEM imaging? } } I'm not a FIB expert, but there are plenty on the microscopy list. There will probably be other people who would like to know the answer to this question, too, so please reply both directly to Penn and to the list. } } ------------- } Philip Oshel } Microscopy Society of America } Ask a Microscopist } www(dot)microscopy(dot)org/resources/ask(dot)cfm } } } } ==============================Original Headers============================== } 5, 73 -- From oshel1pe-at-cmich.edu Fri Feb 7 10:52:13 2020 } 5, 73 -- Received: from NAM04-BN3-obe.outbound.protection.outlook.com (mail-eopbgr680123.outbound.protection.outlook.com [40.107.68.123]) } 5, 73 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 017GqDnD026736 } 5, 73 -- for {microscopy-at-microscopy.com} ; Fri, 7 Feb 2020 10:52:13 -0600 } 5, 73 -- ARC-Seal: i=1; a=rsa-sha256; s=arcselector9901; d=microsoft.com; cv=none; } 5, 73 -- b=da/U+R0sXKlzOTSmd81mndzAIiEF6CYYfnreGverm4M7nWp/g+xp2MHBfLbj7TsJNWtztnrUJ7s3NDz8mgKWbCxwmIiNUX6C/gzQSAjNN9N1+B0zHGww5XQkdiaky/nUnwFfaO/y2QEqLydaiP4dYELc6udXby/GdEeHRAAafRsLSM3PVAXxBOOXl7ZOGWyHK0s7D3IoGjnQlwJ5S+RYspATzHPMCJN9/j+Yk4yp4INPHLk3y+l0fUrSHH6Y4U0YG/fGD9pwkTcnPYsbgN0HDmKATDoU5666L+QdMSO1VehIRShsB19O+2sToE9bPC5HsIkH65LOCJAWxgiU/p3F6A== } 5, 73 -- ARC-Message-Signature: i=1; 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Avoiding surface damage during FIB sample preparation is fairly straight-forward: prior to ever exposing sample ion beam it should be coated with sufficient thickness (~100nm optimal, but } 30nm is a minimal requirement) of some protective layer that will "absorb" ion beam damage.
Following coatings may typically be used for such protective layer, depending on nature of the sample: (a) e-beam deposition of C, Pt, Mo, W, SiOx, etc...; (b) evaporated carbon or metal; (c) sputter coating by Au, Au/Pd, TiO, Cr, Ir, etc...; (d) conductive polymer coating by spinning or ultrasonic nozzle dispensing; (e) ink coating (i.e. "Sharpie trick), etc...
For atomic resolution TEM amorphous layer on the sides of the lamella would also need to be cleaned, but that is already another question.
Happy sample prepping :) Valery
Valery Ray (also with REFINE Lab, UCONN) ========================================== MEO Engineering Company, Inc. DBA "PBS&T" 290 Broadway, Suite 298, Methuen MA 01844 www.partbeamsystech.com www.freudlabs.com E-mail: vray-at-partbeamsystech.com Phone: +1-978-305-0479
On 2/7/2020 11:52 AM, oshel1pe-at-cmich.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } *********************************************************************************** } Forwarded from "Ask a Microscopist" } Please remember that the person asking the question is likely not a member the listserver, and } **any reply should go directly to the poster** as well as to the list. } Using the "reply" function in your email does *not* send your answer to the person asking the question. } Please copy their email address from their question. } *********************************************************************************** } Name: Aubrey Penn } Email: anpenn-at-ncsu.edu } Subject: FIB sample preparation } Your Question: What are the best ways to avoid damaging or outright destroying an epitaxial thin film (~20nm thick) film using Ga ion FIB for atomic resolution STEM imaging? } } I'm not a FIB expert, but there are plenty on the microscopy list. There will probably be other people who would like to know the answer to this question, too, so please reply both directly to Penn and to the list. } } ------------- } Philip Oshel } Microscopy Society of America } Ask a Microscopist } www(dot)microscopy(dot)org/resources/ask(dot)cfm } } } } ==============================Original Headers============================== } 5, 73 -- From oshel1pe-at-cmich.edu Fri Feb 7 10:52:13 2020 } 5, 73 -- Received: from NAM04-BN3-obe.outbound.protection.outlook.com (mail-eopbgr680123.outbound.protection.outlook.com [40.107.68.123]) } 5, 73 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 017GqDnD026736 } 5, 73 -- for {microscopy-at-microscopy.com} ; Fri, 7 Feb 2020 10:52:13 -0600 } 5, 73 -- ARC-Seal: i=1; a=rsa-sha256; s=arcselector9901; d=microsoft.com; cv=none; } 5, 73 -- b=da/U+R0sXKlzOTSmd81mndzAIiEF6CYYfnreGverm4M7nWp/g+xp2MHBfLbj7TsJNWtztnrUJ7s3NDz8mgKWbCxwmIiNUX6C/gzQSAjNN9N1+B0zHGww5XQkdiaky/nUnwFfaO/y2QEqLydaiP4dYELc6udXby/GdEeHRAAafRsLSM3PVAXxBOOXl7ZOGWyHK0s7D3IoGjnQlwJ5S+RYspATzHPMCJN9/j+Yk4yp4INPHLk3y+l0fUrSHH6Y4U0YG/fGD9pwkTcnPYsbgN0HDmKATDoU5666L+QdMSO1VehIRShsB19O+2sToE9bPC5HsIkH65LOCJAWxgiU/p3F6A== } 5, 73 -- ARC-Message-Signature: i=1; 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Email: elena.belluso-at-unito.it
Name: Elena Belluso
Organization: University of Torino - I
Title-Subject: [Filtered] session 13d ìAirborne particles and fibers: characteristics, sources, toxicology, and impacts on ecosystems and human healthî, Goldschmidt 2020
Message: Dear Colleague, We are pleased to invite you to submit an abstract for an oral presentation (or a poster) to the session 13d: AIRBORNE PARTICLES AND FIBERS: CHARACTERISTICS, SOURCES, TOXICOLOGY, AND IMPACTS ON ECOSYSTEMS AND HUMAN HEALTHî (Theme 13 https://goldschmidt.info/2020/program/programViewThemes) at Goldschmidt 2020, the international conference on geochemistry and related subjects, scheduled from 21 to 26 June 2020 in Honolulu, Hawaii.
The key-note speaker of the session is Reto GierÈ (University of Pennsylvania): ìMineralogy, Chemistry, and Toxicology of Particulate Emissions from Combustion Processesî.
Convenors: Elena Belluso (Universit‡ degli Studi di Torino, Torino, Italy), Janice Brahney (Utah State University, Logan, UT, USA), Francesco Di Benedetto (Universit‡ degli Studi di Firenze, Firenze, Italy), Peggy O'Day (University of California, Merced, CA, USA)
Note that the abstract deadline is February, 14, 2020 (https://goldschmidt.info/2020/abstracts)
We look forward to seeing you in Honolulu in June!
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X-from: Richard E. Edelmann {Edelmare-at-miamioh.edu}
Andrew,
There are really only three factors:
(1) The film. Is it old or bad? Has something changed in the development? (temp or developer aged etc.)
(2) Is the Sens seeting on the scope still correct? Which you've chanegd all the way up to 20? That is very high.
Finally I am betting it is:
(3) Is the beam current being correct read by the scope? As Page-1 of the CRT display shows the Curr Dens as received on the screen. Does that adjust when you adjust the brightness control?
--} Secondly, there is an adjustment that is made to the Curr Dens reading when the focusing screen is in the beam path vs when it is out. Does the vale look reasonable in vs out? --} I have had both bad signal from the main viewing screen and the adjustment from the focusing screen in vs out.
On 19 Dec 2019 at 8:52, microscopy.listserver-at-gmail.c wrote:
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Recently, the film started to come out completely } under-exposed. It was discovered that the film exposure setting had } changed to 20 out of 20. Since then, we have tried many different } parameters and have had no positive results as far as useful images. } After adjusting the FSE we have an image again, but now we are } struggling to obtain a scale of contrast. Despite being in-focus and } displaying layers of contrast on screen - the film continues to } develop in a more black-and-white situation. } } We have gone through changing accelerating voltage, new film, new } developing chem, exposure times, the full range of FSE, aperture } adjustments and we are still hitting a wall. Has anyone dealt with } this or (preferably) know how to resolve this issue? 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Richard E. Edelmann, Ph.D., Director Center for Advanced Microscopy & Imaging 9C Upham Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-miamioh.edu http://www.cami.muohio.edu
Your Question: What are the best ways to avoid damaging or outright destroying an epitaxial thin film (~20nm thick) film using Ga ion FIB for atomic resolution STEM imaging?
I'm going to answer this in several parts.
You don't say what the material or the substrate is. I assume that it is a semiconductor. If it is and the sample doesn't have to be site-specific, then the best way to prepare a 20 nm thick epitaxial film and avoid Ga damage is not to use the FIB at all.
1. The absolute best way to prepare your sample with no amorphous damage at all is the Small Angle Cleavage Technique originally developed by John McCaffrey and then modified quite a bit by John and myself. A major disadvantage of this technique is that there is no amorphous damage and so it can be difficult to focus and stigmate in a field emission TEM. I will sometimes put a thin layer of ca carbon on the top surface just to have something amorphous that I can use in the FFT to help focus and stigmata. If you want, I can send you a detailed presentation on how to do the technique. Just send a request to my work email: (scott-dot-d-dot-walck2-dot-ctr-at-mail-dot-mil).
2. The next best way of preparing your sample would be low angle, low energy Ar ion milling developed by Arpad Barna. Now, all the manufacturers of ion mills have low angle, low energy capabilities. Arpad has several publications out that show the amorphous damage at different energies and angles.
3. OK, so you have a FIB and you don't want to learn how to make samples the old fashioned way. You have to protect the top layer from ion damage before putting on the ion beam Pt (or W) protective layer. You can put it on by e-beam deposition. All you need is 200-250 nm thick to stop and ions from hitting the top surface. Just don't let the ion beam hit the surface with too high of a current or for very long (minimize the dosage to the surface or you will erode the protective layer.) If your sample is not site specific and you don't want to have a high Z material next to your very thin layer, you should consider putting carbon down. Any easy way to do this is to use a Sharpie(R) pen and coat the surface before you put it in the FIB. This works, but can lead to open areas and what looks a bit stringy. A better way is to use a carbon coater to put a uniform layer down. We have a Leica ACE 600 and I just measured the thickness of carbon using a double carbon thread and using it all up. It gave a thickness of 390 nm, which is plenty. At any rate, putting the carbon layer next to your thin layer gives a better contrast between the layer of interest and the protective top layer to see it better. The high Z of Pt or W can make it difficult to see a very thin layer at the top of the surface of your sample.
When preparing the sample by FIB, you will have an amorphous damage layer on top and bottom with a thickness that is dependent on the energy of the beam that you last use. The newest FIBs are capable of "polishing" with a 2 keV (or lower) Ga beam to minimize the damage layer. It is done by exposing the two surfaces at an angle of 3∞. Samples prepared this way are pretty good, but remember, there is still an amorphous layer on both the top and bottom surfaces.
There are also other ways of removing FIB damage from the surfaces of FIB lamella using Ar ion milling. In decreasing order of cost:
-E.A. Fischione makes the NanoMill and the PicoMill, which use a scanning Ar beam to polish the surfaces with low energy Ar. They have presentations and product information on these tools.
-Several manufacturers of ion mills, specifically Gatan and Technoorg Linda of recipes for removing damage using their tools. You should contact them for papers and presentations on how to do that.
-Another technique that I patented while working at South Bay Technology is the Plasma Trimming method, which uses the shape of the FIB sample and a bias to create a field that causes ions from a plasma to polish the two surface of the sample. The plasma was generating using Ar gas in a plasma cleaner. This patent is now owned by Ted Pella, Inc. I can provide a Plasma Trimming presentation upon request. (scott-dot-d-dot-walck2-dot-ctr-at-mail-dot-mil)
BTW, you can estimate the amount of damage and depth for different ions, energies, and angles by using SRIM and TRIM calculations. See www.srim.org for more information and to download the software and manual.
I hope that this helps. I'm sure it is more than you need.
-Scott
-----Original Message----- X-from: oshel1pe-at-cmich.edu Sent: Friday, February 7, 2020 11:55 AM To: s.walck-at-comcast.net
**************************************************************************** ******* Forwarded from "Ask a Microscopist" Please remember that the person asking the question is likely not a member the listserver, and **any reply should go directly to the poster** as well as to the list. Using the "reply" function in your email does *not* send your answer to the person asking the question. Please copy their email address from their question. **************************************************************************** ******* Name: Aubrey Penn Email: anpenn-at-ncsu.edu
Hello Aubrey,
Good to see you on the listserv!
In regards to your question, Valery and Scott gave you some good advice. I've always referred students to the following paper: https://www.sciencedirect.com/science/article/abs/pii/S030439911200006X I can give you a copy the next time I see you.
There are a lot of ways to skin a cat, as they say, but it can be challenging to get good FIB samples if you're used to the results generated by tripod polishing and low energy, angle ion milling. The best results I've seen are from FIB samples prepared in a similar manner as in the paper above, followed by a cleanup in a low energy ion mill (Fischione, Gatan, Technoorg-Linda...all are fine). However, the ion mill cleanup is not magic. You can start to etch your liftout if the angle and voltages are not ideal, or if you mill for too long to compensate for a thicker liftout.
Depending on the materials, you may also be able to dip the liftout in a dilute acid or base to thin the liftout, or use a flash electropolishing setup like the one they have at PNNL to reduce the liftout thickness and remove damage layers. Regardless of what post-FIB step you use, you need to do your best to create a thin, flat liftout with a minimal amount of sputter redep in the first place, and that's where that paper gives some valuable tips.
Good luck, Chris
On Fri, Feb 7, 2020 at 10:52 AM {oshel1pe-at-cmich.edu} wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } *********************************************************************************** } Forwarded from "Ask a Microscopist" } Please remember that the person asking the question is likely not a member the listserver, and } **any reply should go directly to the poster** as well as to the list. } Using the "reply" function in your email does *not* send your answer to the person asking the question. } Please copy their email address from their question. } *********************************************************************************** } Name: Aubrey Penn } Email: anpenn-at-ncsu.edu } Subject: FIB sample preparation } Your Question: What are the best ways to avoid damaging or outright destroying an epitaxial thin film (~20nm thick) film using Ga ion FIB for atomic resolution STEM imaging? } } I'm not a FIB expert, but there are plenty on the microscopy list. There will probably be other people who would like to know the answer to this question, too, so please reply both directly to Penn and to the list. } } ------------- } Philip Oshel } Microscopy Society of America } Ask a Microscopist } www(dot)microscopy(dot)org/resources/ask(dot)cfm } } } } ==============================Original Headers============================== } 5, 73 -- From oshel1pe-at-cmich.edu Fri Feb 7 10:52:13 2020 } 5, 73 -- Received: from NAM04-BN3-obe.outbound.protection.outlook.com (mail-eopbgr680123.outbound.protection.outlook.com [40.107.68.123]) } 5, 73 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 017GqDnD026736 } 5, 73 -- for {microscopy-at-microscopy.com} ; Fri, 7 Feb 2020 10:52:13 -0600 } 5, 73 -- ARC-Seal: i=1; a=rsa-sha256; s=arcselector9901; d=microsoft.com; cv=none; } 5, 73 -- b=da/U+R0sXKlzOTSmd81mndzAIiEF6CYYfnreGverm4M7nWp/g+xp2MHBfLbj7TsJNWtztnrUJ7s3NDz8mgKWbCxwmIiNUX6C/gzQSAjNN9N1+B0zHGww5XQkdiaky/nUnwFfaO/y2QEqLydaiP4dYELc6udXby/GdEeHRAAafRsLSM3PVAXxBOOXl7ZOGWyHK0s7D3IoGjnQlwJ5S+RYspATzHPMCJN9/j+Yk4yp4INPHLk3y+l0fUrSHH6Y4U0YG/fGD9pwkTcnPYsbgN0HDmKATDoU5666L+QdMSO1VehIRShsB19O+2sToE9bPC5HsIkH65LOCJAWxgiU/p3F6A== } 5, 73 -- ARC-Message-Signature: i=1; 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-- Transmission Electron Microscopy Lab Manager Analytical Instrumentation Facility (AIF) NC State University https://www.aif.ncsu.edu/ Cell: 267-496-0587
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Title-Subject: [Filtered] We are hiring! Electron Microscopist / Imaging Specialist
Message: Hello! Sivananthan Laboratories, Inc. is a high-tech business incubator focused on promoting economic growth in Illinois and the United States through fostering cutting-edge, fundamental research and development. We work on R&D for AI, infrared detectors, image processing, solar energy, and more. We are currently looking for a successful candidate who is skilled in all aspects on scanning electron microscopy (SEM), transmission electron microscopy (TEM) and scanning transmission electron microscopy (STEM) with experience in image simulation and state-of-the-art processing and analytics packages. If you are interested, please go to this link (http://sivananthanlabs.us/employment/) and apply for this position!
If you have any questions, feel free to email me at mrajasingham-at-sivananthanlabs.us
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Email: xbren-at-uw.edu Name: Shirley Ren
Organization: University of Washington
Title-Subject: [Filtered] Covid-19 virus Message: Dear Colleagues, Has someone found the virus under TEM? Is it possible to do RNA-FISH on thick sections (Epoxy embedded block)? Many thanks,
Shirley
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X-from: Beck, Stephen J. {Stephen.Beck-at-ncc.edu}
Hi David,
Thanks for the reply. I’m not sure how I could get samples to you. My students were just working on getting samples prepared. My microbial/Paramecium prep was canceled by the corona virus. My students and I can’t even get on campus at present. Do you have any “stock” biological samples on hand? Can you even get to your own scopes? My students have required samples to image but I’m sure I’ll have to cut that back given the current situation. They would image 2 soft tissues, a cryofractured soft tissue, hard tissue, botanical sample (leaf stomates), pollen, microbial/Paramecium, insect, diatoms. Perhaps I could do a trial demo with you initially?
Thanks,
Steve
Stephen J. Beck Professor Coordinator, Bio-Imaging Center Electron Microscopy Department of Biology Nassau Community College Garden City, NY 11530
Sent from my iPhone - thanks Steve (1955-2011)!
} On Mar 22, 2020, at 12:46 PM, "microscopy.listserver-at-gmail.com" {microscopy.listserver-at-gmail.com} wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } } X-from: David Huskisson {david-at-emanalytical.co.uk} } } } Hi Stephen, } } We have a couple of our SEMs connected to the internet for remote access. Through this interface, } the microscope is completely controllable from your own PC. We can load your samples and then you or } your students would be able to take control of the instrument and practice from your own homes. } } Let me know how we can help. } } Kind regards, } } /David Huskisson, PhD./ } Project Scientist & Microscopist } } } Alderley Park } Macclesfield } SK10 4TG } } Office: +44 (0) 1625 704 467 } DD: +44 (0) 1625 238 869 } Mob: +44 (0) 7837 718 098 } } www.emanalytical.co.uk {http://www.emanalytical.co.uk/} } } } */Confidentiality Notice: /* } } This message is private and confidential. If received in error, please destroy and notify sender. } Sender does not intend to waive confidentiality or privilege. Dissemination, use of or reliance upon } this email is prohibited when received in error. Email may be susceptible to data corruption, } interception and unauthorised amendment, and no liability is accepted by the sender for any of the } foregoing. It is the recipient’s responsibility to scan the email and any attachment for viruses. } } } } On Sat, Mar 21, 2020 at 1:48 PM {microscopy.listserver-at-gmail.com } {mailto:microscopy.listserver-at-gmail.com} } wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line {http://www.microscopy.com/MicroscopyListserverOn-Line} Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: Stephen.Beck-at-ncc.edu {mailto:Stephen.Beck-at-ncc.edu} } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy to both } Stephen.Beck-at-ncc.edu {mailto:Stephen.Beck-at-ncc.edu} , Microscopy-at-Microscopy.com so that all } Microscopy Listserver Subscribers can } benefit from our collective wisdom } --------------------------------------------------------------------------- } } Email: Stephen.Beck-at-ncc.edu {mailto:Stephen.Beck-at-ncc.edu} Name: Steve Beck } } Organization: Nassau Community College } } Title-Subject: [Filtered] Teaching SEM Online } } Message: Dear Colleagues, } I am teaching my SEM course this semester and like many institutions, we are trying to teach } online/remotely as our campus is closed due to COVID-19. I have administered my midterm exam } online, } however, after that we need to get on the SEM to image the required samples. } Does anyone have any ideas regarding teaching SEM online? 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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both drk-at-shcc.org, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Email: drk-at-shcc.org Name: Doug Keene
Organization: Shriners Hospital for Childern
Title-Subject: [Filtered] I'd like to help with Corona virus research
Message: Hello Everyone,
I'd like to apply my talents in Microscopy (TEM, SEM, Confocal, Micro-CT) to the Corona pandemic. How do I get the word out that I would like to contribute?
Thanks,
Doug Keene Shriner Hospital for Children Portland, Oregon 503-819-3600 (cell)
Login Host: 76.105.214.204 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
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X-from: Straszheim, Warren E [BIOTC] {wesaia-at-iastate.edu}
It seems that you replied to the entire list. Since you did, there should be many scopes in many labs that should be able to help. The FEI scopes are setup to use UltraVNC as part of their RAPID support service. It can be used just as well by lab staff and clients for their own purposes. (In fact, I have used it more for my own benefit than FEI has ever used it for theirs.) It would need to be setup properly with a single computer controlling the computer. Multiple others might be able to watch along. I would offer to help, but I am in the materials science area and would barely know what I am looking at. We also do not have samples handy for demo purposes. I have a counterpart that would have such expertise and samples, but she runs a Hitachi SEM and it is not setup for remote control or viewing. Warren Straszheim, Ph.D., manager Materials Analysis and Research Lab Iowa State University 515-294-8187
-----Original Message----- X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Tuesday, March 24, 2020 8:32 AM To: Straszheim, Warren E [BIOTC] {wesaia-at-iastate.edu}
X-from: Beck, Stephen J. {Stephen.Beck-at-ncc.edu}
Hi David,
Thanks for the reply. I’m not sure how I could get samples to you. My students were just working on getting samples prepared. My microbial/Paramecium prep was canceled by the corona virus. My students and I can’t even get on campus at present. Do you have any “stock” biological samples on hand? Can you even get to your own scopes? My students have required samples to image but I’m sure I’ll have to cut that back given the current situation. They would image 2 soft tissues, a cryofractured soft tissue, hard tissue, botanical sample (leaf stomates), pollen, microbial/Paramecium, insect, diatoms. Perhaps I could do a trial demo with you initially?
Thanks,
Steve
Stephen J. Beck Professor Coordinator, Bio-Imaging Center Electron Microscopy Department of Biology Nassau Community College Garden City, NY 11530
Sent from my iPhone - thanks Steve (1955-2011)!
} On Mar 22, 2020, at 12:46 PM, "microscopy.listserver-at-gmail.com" {microscopy.listserver-at-gmail.com} wrote: } } } } } ---------------------------------------------------------------------- } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } } } X-from: David Huskisson {david-at-emanalytical.co.uk} } } } Hi Stephen, } } We have a couple of our SEMs connected to the internet for remote } access. Through this interface, the microscope is completely } controllable from your own PC. We can load your samples and then you or your students would be able to take control of the instrument and practice from your own homes. } } Let me know how we can help. } } Kind regards, } } /David Huskisson, PhD./ } Project Scientist & Microscopist } } } Alderley Park } Macclesfield } SK10 4TG } } Office: +44 (0) 1625 704 467 } DD: +44 (0) 1625 238 869 } Mob: +44 (0) 7837 718 098 } } www.emanalytical.co.uk {http://www.emanalytical.co.uk/} } } } */Confidentiality Notice: /* } } This message is private and confidential. If received in error, please destroy and notify sender. } Sender does not intend to waive confidentiality or privilege. } Dissemination, use of or reliance upon this email is prohibited when } received in error. Email may be susceptible to data corruption, } interception and unauthorised amendment, and no liability is accepted by the sender for any of the foregoing. It is the recipient’s responsibility to scan the email and any attachment for viruses. } } } } On Sat, Mar 21, 2020 at 1:48 PM {microscopy.listserver-at-gmail.com } {mailto:microscopy.listserver-at-gmail.com} } wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line {http://www.microscopy.com/MicroscopyListserverOn-Line} Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------- } ------ } } X-from: Stephen.Beck-at-ncc.edu {mailto:Stephen.Beck-at-ncc.edu} } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy to both } Stephen.Beck-at-ncc.edu {mailto:Stephen.Beck-at-ncc.edu} , Microscopy-at-Microscopy.com so that all } Microscopy Listserver Subscribers can } benefit from our collective wisdom } } ---------------------------------------------------------------------- } ----- } } Email: Stephen.Beck-at-ncc.edu {mailto:Stephen.Beck-at-ncc.edu} Name: } Steve Beck } } Organization: Nassau Community College } } Title-Subject: [Filtered] Teaching SEM Online } } Message: Dear Colleagues, } I am teaching my SEM course this semester and like many institutions, we are trying to teach } online/remotely as our campus is closed due to COVID-19. I have administered my midterm exam } online, } however, after that we need to get on the SEM to image the required samples. } Does anyone have any ideas regarding teaching SEM online? 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I have tried to visualize E cloi capsular polysaccharide by following the protocol from the book chapter "Electron Microscopy to Study the Fine Structure of the Pneumococcal Cell" by Sven Hammerschmidt and Manfred Rohde from the published book entitled "Streptococcus pneumoniae, Method and protocol" published by Humana Press. I compared alcian blue and ruthenium red in the presence or absence of lysine.
At least in this first trial, ruthenium red in the presence of lysine appeared to be superb among all conditions tested in our study setup. However, based on the EM analysis, I sensed that the preservation of CPS is suboptimal and suggesting room for improvement.
I was wondering if CPS experts could give me any suggestions about how I can improve the presentation of CPS. Many thanks,
Dear Hiro, I would recommend checking the review Ruthenium Red and the Bacterial Glycocaly by Fassel et al. (1999) https://doi.org/10.3109/10520299909047974 it compares several different fixation and staining protocols with indications of what works best for which type of CPS. best regards! Rob
--- Dr. Rob Mesman Post-Doc Department of Microbiology Faculty of Science Radboud University Nijmegen Heyendaalseweg 135 NL-6525 AJ Nijmegen The Netherlands
On 2020-03-24 14:43, microscopy.listserver-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: hiro uryu {hiro.uryu-at-emscopic.com} } } } Dear List, } } I have tried to visualize E cloi capsular polysaccharide by following } the protocol from the book } chapter "Electron Microscopy to Study the Fine Structure of the } Pneumococcal Cell" by Sven } Hammerschmidt and Manfred Rohde from the published book entitled } "Streptococcus pneumoniae, Method } and protocol" published by Humana Press. I compared alcian blue and } ruthenium red in the presence or } absence of lysine. } } At least in this first trial, ruthenium red in the presence of lysine } appeared to be superb among } all conditions tested in our study setup. However, based on the EM } analysis, I sensed that the } preservation of CPS is suboptimal and suggesting room for improvement. } } I was wondering if CPS experts could give me any suggestions about how } I can improve the } presentation of CPS. Many thanks, } } Sincerely, } Hiro } ------ } Kunihiro Uryu, } } } ==============================Original } Headers============================== } 8, 53 -- From microscopy.listserver-at-gmail.com Tue Mar 24 08:33:05 2020 } 8, 53 -- Received: from mail-il1-f178.google.com } (mail-il1-f178.google.com [209.85.166.178]) } 8, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } 02ODX5La027708 } 8, 53 -- for {microscopy-at-microscopy.com} ; Tue, 24 Mar 2020 08:33:05 } -0500 } 8, 53 -- Received: by mail-il1-f178.google.com with SMTP id } r5so11998874ilq.6 } 8, 53 -- for {microscopy-at-microscopy.com} ; Tue, 24 Mar 2020 } 06:33:19 -0700 (PDT) } 8, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 8, 53 -- d=gmail.com; s=20161025; } 8, 53 -- } h=subject:references:to:from:message-id:date:user-agent:mime-version } 8, 53 -- } :in-reply-to:content-language:content-transfer-encoding; } 8, 53 -- bh=RT4nGuTjtbspSuKPh6kiwaS5fInU0499H0wIMC8Wcy4=; } 8, 53 -- } b=a8i1Xg70t6DbybebaIpDmD67r6FLD7UgJmbNbIF17+AYFKSMAD6SrKU31oNbqlVlmk } 8, 53 -- } qqAa8aVYtMSWevsO1ixWsB0XSpQnJCnBbGyL273IpNwrQyJhvxlPEF4WkjnUO33TloAo } 8, 53 -- } vrWq3fAlyEhO2fvvSCQI0M9Gpx/uEjATzrphfd/xymYSANuLTRYZXptfMIdeZf50jPgr } 8, 53 -- } ycJ/wnluKgmeFUfVow8C9Hbaaieg7MxiKGggBecub2LyX6980QVFLN5Qjv8RQc3YGS4/ } 8, 53 -- } P6qRpG83Mdwvx/UV2pp01agxAvZa4Bw5ael3gU2AhwkpWVVXgVrE2pHQBAweKQxiEFO9 } 8, 53 -- hMKQ== } 8, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 8, 53 -- d=1e100.net; s=20161025; } 8, 53 -- } h=x-gm-message-state:subject:references:to:from:message-id:date } 8, 53 -- :user-agent:mime-version:in-reply-to:content-language } 8, 53 -- :content-transfer-encoding; } 8, 53 -- bh=RT4nGuTjtbspSuKPh6kiwaS5fInU0499H0wIMC8Wcy4=; } 8, 53 -- } b=b/Bnly+nsTxA0HQN7J37XrrPa2ckB6Z3tS5T8AyYA2wxJlmhbH/XB7yR4DlVnT5D2P } 8, 53 -- } WukntUke2wlV11xyRKQvZntIhQByFAXozEw/dtE8wLc1x5KAhmyQYmo5Vv2c3Wo7X/G7 } 8, 53 -- } 3BEQjV46o0a8k4HxE++GNKTIx50PbF/UjG+BPws0gsQ/1Q7GbaEFcxCb3kd9oRNGoaoZ } 8, 53 -- } 8gL1Rsi96P/JOvm6aEGrCaWzTCw0Rw+XVek1ImE286gIedxBgXYeH4W058oSytIjH4mk } 8, 53 -- } xzGTRf17BdCQOwWUJvX1MR+WP+0kyVJbnaQoe6v5hSyG3O3pmt3Wlt0OL+QrFJCpXSdG } 8, 53 -- fqXQ== } 8, 53 -- X-Gm-Message-State: } ANhLgQ0lgx7paBjSX48pHPI2WRaYxiNPcUfDYFs5PcXMkInj1qloZ9+P } 8, 53 -- x2nc3YluGQUtapE+w3c3Vzv2L4vY } 8, 53 -- X-Google-Smtp-Source: } ADFU+vvPHf9jO3asg5Gs80mntLOFMBZ2ZgSV9OAtoVA8vI9svoUk9Yn+w2cZRJLnLD7//Jg2z/C/Hw== } 8, 53 -- X-Received: by 2002:a92:7eda:: with SMTP id } q87mr25309489ill.179.1585056798648; } 8, 53 -- Tue, 24 Mar 2020 06:33:18 -0700 (PDT) } 8, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net } ([2603:300a:f04:7100:7174:d30f:fad6:337f]) } 8, 53 -- by smtp.googlemail.com with ESMTPSA id } s7sm5102890iob.53.2020.03.24.06.33.18 } 8, 53 -- for {microscopy-at-microscopy.com} } 8, 53 -- (version=TLS1_3 cipher=TLS_AES_128_GCM_SHA256 } bits=128/128); } 8, 53 -- Tue, 24 Mar 2020 06:33:18 -0700 (PDT) } 8, 53 -- Subject: Fwd: E coli capsule polysaccharide } 8, 53 -- References: } {CADderMKZfYLMkSt0OfnMPDfcw0_o9Uq1OW_LdAFuLenSg61EXQ-at-mail.gmail.com} } 8, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 8, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 8, 53 -- X-Forwarded-Message-Id: } {CADderMKZfYLMkSt0OfnMPDfcw0_o9Uq1OW_LdAFuLenSg61EXQ-at-mail.gmail.com} } 8, 53 -- Message-ID: {af421fd8-b9b8-0147-b29d-6826422afe0c-at-gmail.com} } 8, 53 -- Date: Tue, 24 Mar 2020 08:33:17 -0500 } 8, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.14; } rv:68.0) } 8, 53 -- Gecko/20100101 Thunderbird/68.6.0 } 8, 53 -- MIME-Version: 1.0 } 8, 53 -- In-Reply-To: } {CADderMKZfYLMkSt0OfnMPDfcw0_o9Uq1OW_LdAFuLenSg61EXQ-at-mail.gmail.com} } 8, 53 -- Content-Type: text/plain; charset=utf-8; format=flowed } 8, 53 -- Content-Language: en-US } 8, 53 -- Content-Transfer-Encoding: 8bit } ==============================End of - } Headers==============================
Hi everyone, I have a full FEI remote package that we bought with our Themis Z but I wondered if there was another solution to remote TEM that people are using. I have a spare set of hand panels already.
Chris He/Him/His Christopher J. Gilpin Ph.D. Director, Life Science Microscopy Facility Purdue University Whistler Hall of Agriculture Research, Room S052 170 S. University St West Lafayette, IN 47907 765-494-7750 gilpin-at-purdue.edu lsmf-at-purdue.edu reaches everyone in the facility
Dear Dr. Kunihiro Uryu, seconding Dr. Rob Mesman: fixation conditions…
Unfortunately not in possession of the protocol Dr. Kunihiro Uryu quoted*) only „active working“ colleagues might have the protocol in their collection, read and apply or have an answer for your request.
*) only for convenience: https://www.ncbi.nlm.nih.gov/pubmed/30929202 Methods Mol Biol. 2019;1968:13-33. doi: 10.1007/978-1-4939-9199-0_2.
Electron Microscopy to Study the Fine Structure of the Pneumococcal Cell. Hammerschmidt S1, Rohde M2. Author information Abstract Electron microscopy allows for studying bacterial ultrastructure at high resolutions. Two types of electron microscopes are used for this purpose. The transmission electron microscope allows for access to inner bacterial ultrastructure when imaging ultrathin sections as well as cell wall-attached structures by negative staining, whereas scanning electron microscopy allows for the detection of structures on the bacterial cell surface alone or to study the interplay between pneumococci and their host cells. This chapter deals with recommendations for well-adapted methodologies to examine pneumococcal ultrastructure in detail. Especially, we focus on the preservation of the pneumococcal capsular polysaccharide, which represents an important virulence factor of pneumococci. Since capsules are highly hydrated structures, the introduction of a new fixation protocol involving lysine acetate, ruthenium red, and osmium (LRR fixation) results in a very well-preserved capsular structure in such a way that the amount of capsular material bound on the bacterial surface can be compared within different serotypes. In our method, capsular ultrastructure is preserved without the need for serotype-specific antibodies, which have been used in other studies to preserve the pneumococcal capsule. In addition, the new LRR fixation allows for studying the presence or absence of capsular material during adhesion and invasion of pneumococci on epithelial or endothelial host cells in cell culture experiments. KEYWORDS: Critical point drying; Cryo-FESEM; Field emission scanning electron microscopy; Infection; LRR embedding; LRWhite resin; Pneumococcal capsule; Pneumococci; Transmission electron microscopy PMID: 30929202 DOI: 10.1007/978-1-4939-9199-0_2 [Indexed for MEDLINE] ⇒SPRINGER: https://link.springer.com/protocol/10.1007%2F978-1-4939-9199-0_2
It would be interesting whether you tried "additives" like e.g. TA, La+++ and / or PPD (at least for a part of your specimens) with regard to fixation of the bacteria**) (ok, I know that E. coli is something different from....but you might have a look into my Poster https://www.researchgate.net/publication/215824406_Purpura_fulminans_ultrastructural_findings_by_transmission_electron_microscopy_in_four_cases_with_special_reference_to_fixation_of_skin_biopsies and the oral presentation, uploaded into RG https://www.researchgate.net/publication/281120633_Oral_Lecture_PURPURA_FULMINANS_Ultrastructural_findings_by_Transmission_Electron_Microscopy_in_4_cases_with_special_reference_to_fixation_of_skin_biopsies
Eventually you might have tried also the variation (".....substituting ruthenium red with a 0.5% alcian blue pyridine variant (pH 7.2).), cf. https://www.researchgate.net/publication/313875937_Molecular_characterization_of_invasive_capsule_null_Neisseria_meningitidis_in_South_Africa https://www.researchgate.net/figure/Transmission-electron-micrographs-showing-the-presence-of-surface-capsular-polysaccharide
Beware of Covid-19/SARS-CoV-2 infection, keep distance and STAY WELL,
best wishes AND best regards
Wolfgang Muß (MUSS) Retired Member of MSA SALZBURG, Austria
**) what I wanted to say with this is only pointing to other possibilities for retaining otherwise eluted "cellular or matricial substrate(s) in preparation for TEM.... (Sorry if I bothered anyone in this time of obviously global crisis)
====================================================================== Von: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Gesendet: Dienstag, 24. März 2020 15:10 An: wij.muss-at-aon.at Betreff: [Microscopy] Re: E coli capsule polysaccharide
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X-from: Rob Mesman {R.Mesman-at-science.ru.nl}
Dear Hiro, I would recommend checking the review Ruthenium Red and the Bacterial Glycocaly [Glycocalyx] by Fassel et al. (1999) https://doi.org/10.3109/10520299909047974 it compares several different fixation and staining protocols with indications of what works best for which type of CPS. best regards! Rob
--- Dr. Rob Mesman Post-Doc Department of Microbiology Faculty of Science Radboud University Nijmegen Heyendaalseweg 135 NL-6525 AJ Nijmegen The Netherlands
On 2020-03-24 14:43, microscopy.listserver-at-gmail.com wrote: } ---------------------------------------------------------------------- } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } X-from: hiro uryu {hiro.uryu-at-emscopic.com} } } } Dear List, } } I have tried to visualize E cloi capsular polysaccharide by following } the protocol from the book chapter "Electron Microscopy to Study the } Fine Structure of the Pneumococcal Cell" by Sven Hammerschmidt and } Manfred Rohde from the published book entitled "Streptococcus } pneumoniae, Method and protocol" published by Humana Press. I } compared alcian blue and ruthenium red in the presence or absence } of lysine. } } At least in this first trial, ruthenium red in the presence of lysine } appeared to be superb among all conditions tested in our } study setup. However, based on the EM analysis, I sensed that the } preservation of CPS is suboptimal and suggesting room for improvement. } } I was wondering if CPS experts could give me any suggestions about how } I can improve the presentation of CPS. 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==============================Original Headers============================== 23, 20 -- From wij.muss-at-aon.at Tue Mar 24 09:57:51 2020 23, 20 -- Received: from bsmtp2.bon.at (bsmtp2.bon.at [213.33.87.16]) 23, 20 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 02OEvoCG026169 23, 20 -- for {Microscopy-at-Microscopy.com} ; Tue, 24 Mar 2020 09:57:51 -0500 23, 20 -- Received: from MussTHINK (unknown [93.83.25.98]) 23, 20 -- by bsmtp2.bon.at (Postfix) with ESMTPSA id 48mvWJ0Vwcz5tl9 23, 20 -- for {Microscopy-at-Microscopy.com} ; Tue, 24 Mar 2020 15:58:03 +0100 (CET) 23, 20 -- From: "MUSS Wolfgang Dr. phil./PhD \(OR i.R, retired\)" {wij.muss-at-aon.at} 23, 20 -- To: {Microscopy-at-Microscopy.com} 23, 20 -- Subject: [Microscopy] Re: E coli capsule polysaccharide 23, 20 -- Date: Tue, 24 Mar 2020 15:58:04 +0100 23, 20 -- Message-ID: {007201d601ec$9f1fa850$dd5ef8f0$-at-aon.at} 23, 20 -- MIME-Version: 1.0 23, 20 -- Content-Type: text/plain; 23, 20 -- charset="UTF-8" 23, 20 -- X-Mailer: Microsoft Outlook 15.0 23, 20 -- Thread-Index: AdYB7EW7KJd4afC2S/qdEozFvxB1WQ== 23, 20 -- Content-Language: de 23, 20 -- Content-Transfer-Encoding: 8bit 23, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id 02OEvoCG026169 ==============================End of - Headers==============================
While there are many software solutions for sharing desktops, they all fail a similar hurdle - you have to have your microscope PC connected to the network directly. This is NOT a good idea as your Themis computer will not be protected from the internet.
Over the last few months I've been suing a different solution on our "Classic" Titan cubed. Making use of KVM over IP gear. These consist of sender boxes that have HDMI and USB (and audio) inputs that connect (and can be powered by) Cat5 cables to your network. Then any number of receiver boxes can be used with HDMI/USB outputs anywhere you have a network port. These systems have MANY advantages over the FEI remote software;
1) They are FAST - they do real time up to 4k - not just 10fps you will be lucky to get over VNC 2) There is no intrinsic connection between the microscope and the network - as such there is no security issue (except if someone managed to work out your IP/passwords for the boxes, or put a virus on a USB stick - but this is just be careful..) 3) You can have multiple receivers for every sender - so many people can watch each instrument. 4) USB can be used for the hand panels, keyboard and mice for the computers. 5) Partner these with HDMI switches behind monitors and you can switch between local/remote super quickly. 6) They are microscope/instrument agnostic - if the instrument uses USB and HDMI etc it will work.
Downsides;
1) They work with little configuration inside a subnet (such an individual building - our situation where we have been running the instrument from multiple different locations due to building work) but if you are outside the subnet you'll need some help from your network people. 2) The boxes cost - on the order of $500 for each box. But compared to a microscope.. 3) And you'll need one sender for each screen.
I've been using boxes made by Kramer (I have NO connection to this company) but there are many manufacturers (Lindy, geffen etc). They are mostly used is point of sale displays (all those screens at malls and movie theatres) so they are pretty ubiquitous. I have three boxes (left screen, right screen and accessory screen which runs Gatan, Bruker, EMPAD AND support computer through a remote controlled switch). This has worked really well. Let me know if you have any follow up questions.
Cheers
Matthew
-- Dr Matthew Weyland Joint Deputy Director - Monash Centre for Electron Microscopy Associate Professor - Department of Materials Science and Engineering
Monash Centre for Electron Microscopy 10 Innovation Walk, Clayton Campus Monash University Clayton VIC 3800 Australia
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Hi everyone, I have a full FEI remote package that we bought with our Themis Z but I wondered if there was another solution to remote TEM that people are using. I have a spare set of hand panels already.
Chris He/Him/His Christopher J. Gilpin Ph.D. Director, Life Science Microscopy Facility Purdue University Whistler Hall of Agriculture Research, Room S052 170 S. University St West Lafayette, IN 47907 765-494-7750 gilpin-at-purdue.edu lsmf-at-purdue.edu reaches everyone in the facility
After 12+ great years at the UBC BioImaging Facility, I am moving on to a new position in the private sector next month! As such, my position here is available now and has been posted to the UBC HR website:
This is a great opportunity for someone with experience in various advanced TEM techniques including electron tomography, cryo TEM, HRTEM, and their associated sample prep and image processing techniques. We have also just moved the facility into brand new lab space, so the world will be your oyster! There is opportunity to work with our SEM and optical microscope instruments as well.
Concurrently, we are also looking for an optical microscopy specialist to run our three laser scanning confocal instruments, including an Olympus Multiphoton system, a Perkin-Elmer spinning disk, and their associated equipment. https://www.hr.ubc.ca/careers-postings/staff-s.php Posting ID: 36794
I am happy to answer other questions about the TEM tech position, but please direct any formal inquiries to our facility manager, Miki Fujita. BIF.manager-at-ubc.ca Cheers, Bradford Ross Electron Microscopy Technician BioImaging Facility University of British Columbia Biological Sciences Rm. 0120 6270 University Blvd. Vancouver, B.C. V6T 1Z4 phone 604-822-6996 ==============================Original Headers============================== 7, 47 -- From bradford.ross-at-botany.ubc.ca Tue Feb 11 14:26:05 2020 7, 47 -- Received: from vmaprod2.mail-relay.ubc.ca (vmaprod2.mail-relay.ubc.ca [142.103.117.133]) 7, 47 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 01BKQ2wI000320 7, 47 -- for {microscopy-at-microscopy.com} ; Tue, 11 Feb 2020 14:26:02 -0600 7, 47 -- Received: from vmaprod2.mail-relay.ubc.ca (localhost.localdomain [127.0.0.1]) 7, 47 -- by localhost (Email Security Appliance) with SMTP id B03D162D115_E42FF10B 7, 47 -- for {microscopy-at-microscopy.com} ; Tue, 11 Feb 2020 19:22:56 +0000 (GMT) 7, 47 -- Received: from mx3.mail-relay.ubc.ca (lbpglfsc01gstp01-ents01-f5-vrfglue-float.systems.it.ubc.ca [10.45.24.97]) 7, 47 -- (using TLSv1.2 with cipher ECDHE-RSA-AES256-GCM-SHA384 (256/256 bits)) 7, 47 -- (Client CN "mx3.mail-relay.ubc.ca", Issuer "mx3.mail-relay.ubc.ca" (not verified)) 7, 47 -- by vmaprod2.mail-relay.ubc.ca (Sophos Email Appliance) with ESMTPS id 5C0C962A5FB_E42FF10F 7, 47 -- for {microscopy-at-microscopy.com} ; Tue, 11 Feb 2020 19:22:56 +0000 (GMT) 7, 47 -- Received: from smtp.mail.ubc.ca (lbpglfsc01gstp01-ents01-f5-vrfglue-float.systems.it.ubc.ca [10.45.24.97]) 7, 47 -- (using TLSv1.2 with cipher AES256-GCM-SHA384 (256/256 bits)) 7, 47 -- (Client did not present a certificate) 7, 47 -- by mx3.mail-relay.ubc.ca (Postfix) with ESMTPS id BC8B415F648 7, 47 -- for {microscopy-at-microscopy.com} ; Tue, 11 Feb 2020 11:22:56 -0800 (PST) 7, 47 -- Received: from EXCH-SRV02AP.ead.ubc.ca (10.19.216.28) by 7, 47 -- EXCH-SRV01BP.ead.ubc.ca (10.19.216.29) with Microsoft SMTP Server 7, 47 -- (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_128_GCM_SHA256) id 7, 47 -- 15.1.1847.3; Tue, 11 Feb 2020 11:22:51 -0800 7, 47 -- Received: from EXCH-SRV05AP.ead.ubc.ca (10.19.216.49) by 7, 47 -- EXCH-SRV02AP.ead.ubc.ca (10.19.216.28) with Microsoft SMTP Server 7, 47 -- (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_128_GCM_SHA256) id 7, 47 -- 15.1.1847.3; Tue, 11 Feb 2020 11:18:55 -0800 7, 47 -- Received: from EXCH-SRV05AP.ead.ubc.ca ([fe80::c0d9:94b3:5718:3f80]) by 7, 47 -- EXCH-SRV05AP.ead.ubc.ca ([fe80::c0d9:94b3:5718:3f80%4]) with mapi id 7, 47 -- 15.01.1847.005; Tue, 11 Feb 2020 11:18:55 -0800 7, 47 -- From: "Ross, Bradford" {bradford.ross-at-botany.ubc.ca} 7, 47 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 7, 47 -- Subject: Core Facility Research Tech Position Available 7, 47 -- Thread-Topic: Core Facility Research Tech Position Available 7, 47 -- Thread-Index: AQHV4Qy+GzcqnRAw0k+U9Bc56Rhg7A== 7, 47 -- Date: Tue, 11 Feb 2020 19:18:55 +0000 7, 47 -- Message-ID: {e676603a70694f9a847ea3ac343a7adc-at-botany.ubc.ca} 7, 47 -- Accept-Language: en-US, en-CA 7, 47 -- Content-Language: en-US 7, 47 -- X-MS-Has-Attach: 7, 47 -- X-MS-TNEF-Correlator: 7, 47 -- x-originating-ip: [137.82.85.238] 7, 47 -- x-pmwin-version: 4.0.4, Antivirus-Engine: 3.77.1, Antivirus-Data: 5.72 7, 47 -- Content-Type: text/plain; charset="iso-8859-1" 7, 47 -- MIME-Version: 1.0 7, 47 -- X-PMWin-Version: 4.0.4, Antivirus-Engine: 3.77.1, Antivirus-Data: 5.72 7, 47 -- X-SASI-RCODE: 200 7, 47 -- Content-Transfer-Encoding: 8bit 7, 47 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id 01BKQ2wI000320 ==============================End of - Headers==============================
Does anybody happen to have an SAD-aperture assembly (aka field limiting aperture assembly, manual version) for a Jeol JEM2100F lying outside the microscope at the moment?
I would be really interested in a photo of it with some measurements, mainly the diameter and length of the rod.
Alternatively, a sketch with dimensions would also be great.
All the best,
Philip
PS: I'm trying to avoid opening up the microscope and getting drawings from Jeol is a lengthy procedure.
==============================Original Headers============================== 15, 49 -- From koeck-at-kth.se Tue Feb 18 09:24:18 2020 15, 49 -- Received: from smtp-3.sys.kth.se (smtp-3.sys.kth.se [130.237.48.192]) 15, 49 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 01IFOHAs017009 15, 49 -- for {microscopy-at-microscopy.com} ; Tue, 18 Feb 2020 09:24:18 -0600 15, 49 -- Received: from smtp-3.sys.kth.se (localhost.localdomain [127.0.0.1]) 15, 49 -- by smtp-3.sys.kth.se (Postfix) with ESMTP id 25879A242 15, 49 -- for {microscopy-at-microscopy.com} ; Tue, 18 Feb 2020 15:21:34 +0100 (CET) 15, 49 -- X-Virus-Scanned: by amavisd-new at kth.se 15, 49 -- Received: from smtp-3.sys.kth.se ([127.0.0.1]) 15, 49 -- by smtp-3.sys.kth.se (smtp-3.sys.kth.se [127.0.0.1]) (amavisd-new, port 10024) 15, 49 -- with LMTP id VRJC0qWBXGaA for {microscopy-at-microscopy.com} ; 15, 49 -- Tue, 18 Feb 2020 15:21:33 +0100 (CET) 15, 49 -- Received: from exdb02.ug.kth.se (exdb02.ug.kth.se [192.168.32.112]) 15, 49 -- by smtp-3.sys.kth.se (Postfix) with ESMTPS id 3DAA3A281 15, 49 -- for {microscopy-at-microscopy.com} ; Tue, 18 Feb 2020 15:21:32 +0100 (CET) 15, 49 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=kth.se; s=default; 15, 49 -- t=1582035693; bh=YFkqfWEL7AxKOjOPgs44CMGZs78Sm//g4ePcYboHFhU=; 15, 49 -- h=From:To:Subject:Date; 15, 49 -- b=PARXhxD1lKqXFGxroRSsrSkYZEJp+8zrNkFQLUmRAW48F9z85oFG6uRKz1FlY0Qa8 15, 49 -- +ANRgA9fYyVIeUixwBD8Tbvv5I2I5la5mr4+pG1IajwIwhFrmA4MAg/H1BiNj2GzB5 15, 49 -- fyNSqfnSHO5A9b8RiNj9YveW6OzNLb9khlg0sTBw= 15, 49 -- Received: from exdb03.ug.kth.se (192.168.32.113) by exdb02.ug.kth.se 15, 49 -- (192.168.32.112) with Microsoft SMTP Server (TLS) id 15.0.1473.3; Tue, 18 Feb 15, 49 -- 2020 15:21:08 +0100 15, 49 -- Received: from exdb01.ug.kth.se (192.168.32.111) by exdb03.ug.kth.se 15, 49 -- (192.168.32.113) with Microsoft SMTP Server (TLS) id 15.0.1473.3; Tue, 18 Feb 15, 49 -- 2020 15:21:08 +0100 15, 49 -- Received: from exdb01.ug.kth.se ([192.168.32.111]) by exdb01.ug.kth.se 15, 49 -- ([192.168.32.111]) with mapi id 15.00.1473.005; Tue, 18 Feb 2020 15:21:08 15, 49 -- +0100 15, 49 -- From: =?utf-8?B?UGhpbGlwIEvDtmNr?= {koeck-at-kth.se} 15, 49 -- To: "3dem-at-ncmri.ucsd.edu" {3dem-at-ncmri.ucsd.edu} , 15, 49 -- "microscopy-at-microscopy.com" 15, 49 -- {microscopy-at-microscopy.com} 15, 49 -- Subject: SAD-aperture assembly 15, 49 -- Thread-Topic: SAD-aperture assembly 15, 49 -- Thread-Index: AQHV5mahbPDcSWghfEuLD4b8dHeypg== 15, 49 -- Date: Tue, 18 Feb 2020 14:21:07 +0000 15, 49 -- Message-ID: {1582035668079.3393-at-kth.se} 15, 49 -- Accept-Language: sv-SE, en-US 15, 49 -- Content-Language: sv-SE 15, 49 -- X-MS-Has-Attach: 15, 49 -- X-MS-TNEF-Correlator: 15, 49 -- x-ms-exchange-transport-fromentityheader: Hosted 15, 49 -- x-originating-ip: [130.229.133.148] 15, 49 -- Content-Type: text/plain; charset="utf-8" 15, 49 -- MIME-Version: 1.0 15, 49 -- Content-Transfer-Encoding: 8bit 15, 49 -- X-MIME-Autoconverted: from base64 to 8bit by microscopy.com id 01IFOHAs017009 ==============================End of - Headers==============================
The Coln-Ramos lab at Yale University is looking for a research associate to provide ongoing electron microscopy work for a variety of projects in the lab. Under the direction of the Principal Investigator and working in collaboration with laboratory personnel, this position will be in charge of the design, collection, optimization and interpretation of electron microscopy research in the Coln-Ramos lab as it pertains to the ultrastructure of C. elegans nervous system. The position will prepare and process C. elegans worms for electron microscopy to better understand the function and structure of the C. elegans nervous system. More information about the position can be found here: https://www.nature.com/naturecareers/job/electron-microscopy-research-associate-yale-university-715443
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both tprozoro-at-ameslab.gov, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Title-Subject: [Filtered] Postdoctoral position at the Ames Laboratory
Message: The Division of Materials Science and Engineering (DMSE) at the Ames Laboratory, a Department of Energy National Laboratory affiliated with Iowa State University, is searching for a qualified Postdoctoral Research Associate.
We are looking for a motivated postdoctoral research associate to work on two projects focused on characterization of structure and liquid phase dynamics of beam-sensitive materials by using electron microscopy in-situ. The first project involves spatio-chemical characterization of polymer-nanoparticle complexes and DNA origami nanostructures in liquid phase. The second project involves monitoring cation mobility in low-contrast layered minerals in liquid phase and in-situ. We are looking for applicants with hands-on experience using aberration-corrected scanning transmission electron microscopy (S/TEM), electron energy loss spectroscopy (EELS), and energy dispersive spectroscopy (EDS). More details and application instructions can be found here: https://isu.wd1.myworkdayjobs.com/en-US/IowaStateJobs/job/Ames-IA/Postdoctoral-Research-Associate---Ames-Laboratory_R1870
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Dear All, I apologize for a job posting that appears containing an invalid link. Thanks for those of you who brought it to my attention. Here is the link to the Yale HR website, and it works. https://sjobs.brassring.com/TGnewUI/Search/Home/Home?partnerid=25053&siteid=5248#jobDetails=1405083_5248.
Thanks you for your attention.
Xinran Nick Liu, M.D. & Ph.D. Director, Center for Cellular and Molecular Imaging Bio & Cryo Electron Microscopy Core Facilities Yale University School of Medicine Office: (203) 785-4050 Lab: (203) 785-5390 http://medicine.yale.edu/ccmi/em
Dear Collogues, The Colón-Ramos lab at Yale University is looking for a research associate to provide ongoing electron microscopy work for a variety of projects in the lab. Under the direction of the Principal Investigator and working in collaboration with laboratory personnel, this position will be in charge of the design, collection, optimization and interpretation of electron microscopy research in the Colón-Ramos lab as it pertains to the ultrastructure of C. elegans nervous system. The position will prepare and process C. elegans worms for electron microscopy to better understand the function and structure of the C. elegans nervous system. More information about the position can be found here: https://www.nature.com/naturecareers/job/electron-microscopy-research-associate-yale-university-715443 Thanks for your attention.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both dkc25-at-cam.ac.uk, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Email: dkc25-at-cam.ac.uk Name: Darran Clements
Organization: University of Cambridge
Title-Subject: [Filtered] Stitching confocal volumes of organ in 3 dimensions
Message: Hi All, just wanted to throw out a general question to the list. We are currently considering embarking on a project where we have been asked to reconstruct an organ from mosaics of confocal volumes in serial sections. We can stitch volumes in each section together easily enough, however, I wanted to ask if anyone else has experience of then stitching these kinds of volumes together to reconstruct the whole organ volume, and if so, could they impart some advice on what they found works best.
We are looking at slices of tissue around 100 microns thick and around 80-100 of these volumes stitched together, then stitching multiple of these volume mosaics, one above the other, to reconstruct the tissue.
We're mainly looking at the software aspect of this but of course, tangential replies and alternative ideas are more than welcome too.
Many thanks Darran
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By the way, the solid angle would be the active area of the detector divided by the square of the distance to specimen.
Krassimir.
} On Feb 19, 2020, at 2:45 PM, nizets2-at-yahoo.com wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear colleagues, } } I'd have a simple question regarding the detector area of EDX (EDS) systems in SEM: } Clearly the largest the detector, the highest the sensitivity for everything else equal but what if everything else was NOT equal? } } One clear example: } } - detector 1: 30mm², optimal distance to pole piece 10mm } - detector 2: 150mm², optimal distance to pole piece 15mm } } Here the largest detector is also the furthest! } } I don't know how to appreciate the influence of the 2 factors, detector area and work distance, on the final sensitivity of the system. } In other words, is the extra $$$ for the larger detector worth it if the work distance is at the same time increased? } } Many thanks in advance. } Stephane } } } ==============================Original Headers============================== } 8, 36 -- From nizets2-at-yahoo.com Wed Feb 19 16:40:48 2020 } 8, 36 -- Received: from sonic306-48.consmr.mail.gq1.yahoo.com (sonic306-48.consmr.mail.gq1.yahoo.com [98.137.68.111]) } 8, 36 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 01JMelux001954 } 8, 36 -- for {microscopy-at-microscopy.com} ; Wed, 19 Feb 2020 16:40:48 -0600 } 8, 36 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1582148289; bh=IDWrBC77DmQ8crCMi3VeBrepgESkQS9w38Lnx1B7gCA=; h=Date:From:To:Subject:References:From:Subject; b=Z5qqm6sz+L42XrA1L8rOQdfY6SYUoPrW1kaa7PuNRtfJpQgkW/usMK/A5gNKEzH/L/AwjzemL9yhlSoEZv6GJxXtgjHmqRu9bPrd2u4NHmp+VRGpoEZb+zV2kwx2sPwJKOR9NRtnRpc/1+wSzS7vGk8ya71C0gMU7f4pf2vwSqNtOME0KSJbR4SjjcFnp8PaxFUmsjP0ijnoE76mBjiWHqiyBJn1j6cBf4bJXJPQlQiZ4F1OlliLFOYk81jM0pQPgwgqF/om/P8+9OKz9a1xIeLBnKkwnKt2dBjhZeZ1tFpruTLdR1LWZjIPOmWKZfV82Qmgcek1O7KtFH4F/x9e8w== } 8, 36 -- X-YMail-OSG: KcDAD2IVM1miC7U6QuOqrAbAE3ZpEChGohU34TtHPElCud1VQEfiO8GR6tBqwW0 } 8, 36 -- WqHRIUFFUqKg6sXLtvXKj7d0PS7vHc41I8dv1SCKucrTUKw7RpEJLkcussukkz9yd.b0.uvFE9xs } 8, 36 -- FfqtjLBqgAV0mh4QmDfJI.quhjfONSWBwUL30JlVTe.EqrE.llX75rjZ.Je8vC7uJWbwlXo3VzjG } 8, 36 -- pbzdcFWDSous2wcm9RfCGRJBEL9HGHDSTBNQ2bOcOXsGAr_mpacd0kmX9CpOiP910On_xWwh_37y } 8, 36 -- lkOPBXDHES_X.MLBsNz8m1JxPj4vRM_dG1JgiOCbVyePA2V78i2htZvBdJ4Yfoxfn5EBg_4TdfZ4 } 8, 36 -- sgUy8IbcjKGCGKjjzSKihRM6D.QMwbZB9GruGnIa4rUWfU6Wv18eKYfBYHvhufSyCcnp4WESYX6B } 8, 36 -- tstgB93l.t8f2E9XV2XZHkEF980bP3X6oS1h5zZ_0EqPPdynJPFdMRCz7yPyJl4f3zgcNB3Zd1XM } 8, 36 -- 08yETtbZoTjTA4eGqP8RLf2R5.FKfsc02UPriDFjnw3GURjL7zbDdvSUe5RWp8PXhTQ_0ztmZS0Q } 8, 36 -- taC2HKOh2369e6kypne13SBNyG45YVie63HGXsuUl6bzgltnS7zfvnFIRxTDMJlksEt.WhMXPgxU } 8, 36 -- EUE7d9XL9UBj1ed.JkvAvHGsZF8CS8kkJz5.JBFxXglEYAe.7kWpvhEc0jkOYRzpJmePAXNS.ivY } 8, 36 -- t_bLjEMwTKgnDLL9Z0F9f_t0IvpA7iYCaw2RZdARCcBLK1p3gtipfMinYe1O2xI7R1NRSJ4g9C_L } 8, 36 -- wBpY6G4I4bAzKaDvourEDIO.7U8_Htci6d1bPkl7IUB6btxD6coaH8yg5csJyjSGtzwJf7Z4jtUt } 8, 36 -- 7d_m.e5hGkqaNHTxM8531QqFJ68A6yH7isf69J3I37mlUp.di8feQpIZtGn0sFpZ.N9dY882OkA1 } 8, 36 -- LA0nDRw4aivOyeN9XTIeMQqtRv_kBmInKN8JV2r2axc_6qQC62IdNmFGYh_E4J5Lm4IPxm_7Zaa6 } 8, 36 -- a6eKuKdyvm1vEvF0VClCeTbqLuGVyX06wLxvv4g7rivJpxabKL2.ajnTDx_Cbf90inh7_LmUMQ4M } 8, 36 -- xlNjyk7kLSUwn_g0vrhNaliH_hUzDaoNgHdX8_ESQKmnOBzHA4KC250WyeRW3n4oSZSYkzKC0Isg } 8, 36 -- 7agAb9UJc_VkXWiqhKYVFOfAvaIq3he7w7iXAvn2bqgQkgPSsyPo29MEgXs9q88zmDKKAZf.GLxp } 8, 36 -- FKLHhT8dKDw2131DHpN4TwheOxUpohl9VWngZ6XU480qtLJrRt8IYkj9t7jiGauPcf.BABJPTm2w } 8, 36 -- 2fsHZFAYz9cs.QwKT6xG9viLb6vEcZA-- } 8, 36 -- Received: from sonic.gate.mail.ne1.yahoo.com by sonic306.consmr.mail.gq1.yahoo.com with HTTP; Wed, 19 Feb 2020 21:38:09 +0000 } 8, 36 -- Date: Wed, 19 Feb 2020 21:36:07 +0000 (UTC) } 8, 36 -- From: Stephane Nizet {nizets2-at-yahoo.com} } 8, 36 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} } 8, 36 -- Message-ID: {317764846.5589118.1582148167228-at-mail.yahoo.com} } 8, 36 -- Subject: question about EDS in SEM } 8, 36 -- MIME-Version: 1.0 } 8, 36 -- Content-Type: text/plain; charset=UTF-8 } 8, 36 -- References: {317764846.5589118.1582148167228.ref-at-mail.yahoo.com} } 8, 36 -- X-Mailer: WebService/1.1.15199 YMailNorrin Mozilla/5.0 (Windows NT 10.0; Win64; x64; rv:72.0) Gecko/20100101 Firefox/72.0 } 8, 36 -- Content-Transfer-Encoding: 8bit } 8, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id 01JMelux001954 } ==============================End of - Headers==============================
We are looking after a traceable standard specimen for TEM calibration. We had a MAG*I*CAL sample that got damaged, and it is in a temporary backorder at the EMSDiasum website.
Does any of you know and have used another traceable standard specimen for TEM calibration?
Thank you,
-- Erico Freitas
Physicist/Microscopist at Center of Microscopy Universidade Federal de Minas Gerais (UFMG) Av. Antônio Carlos, 6627, Campus Pampulha, Belo Horizonte, MG, Brazil. ZIP Code 31270-901. +55-31-3409-7573 +55-31-3409-7575
Coordinator:Transmission Electron Microscopy laboratory
Go to this WWW site. It is an on-line solid angle calculator for XEDS systems.
http://www.zaluzec.com/NJZTools/
It will give you an unbiased calculation of solid angles.
Importantly I must contradict Krassimir, Area/distance^2 is incorrect, that is an approximation which quickly breaks down for large detectors and small distances.
On that Solid Angle WWW site is also a link to a paper you might want to read.
Cheers
Nestor Your Friendly Neighborhood SysOp
On 2/20/20 9:40 AM, nizets2-at-yahoo.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear colleagues, } } I'd have a simple question regarding the detector area of EDX (EDS) systems in SEM: } Clearly the largest the detector, the highest the sensitivity for everything else equal but what if everything else was NOT equal? } } One clear example: } } - detector 1: 30mm², optimal distance to pole piece 10mm } - detector 2: 150mm², optimal distance to pole piece 15mm } } Here the largest detector is also the furthest! } } I don't know how to appreciate the influence of the 2 factors, detector area and work distance, on the final sensitivity of the system. } In other words, is the extra $$$ for the larger detector worth it if the work distance is at the same time increased? } } Many thanks in advance. } Stephane } } } ==============================Original Headers============================== } 8, 36 -- From nizets2-at-yahoo.com Wed Feb 19 16:40:48 2020 } 8, 36 -- Received: from sonic306-48.consmr.mail.gq1.yahoo.com (sonic306-48.consmr.mail.gq1.yahoo.com [98.137.68.111]) } 8, 36 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 01JMelux001954 } 8, 36 -- for {microscopy-at-microscopy.com} ; Wed, 19 Feb 2020 16:40:48 -0600 } 8, 36 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1582148289; bh=IDWrBC77DmQ8crCMi3VeBrepgESkQS9w38Lnx1B7gCA=; h=Date:From:To:Subject:References:From:Subject; b=Z5qqm6sz+L42XrA1L8rOQdfY6SYUoPrW1kaa7PuNRtfJpQgkW/usMK/A5gNKEzH/L/AwjzemL9yhlSoEZv6GJxXtgjHmqRu9bPrd2u4NHmp+VRGpoEZb+zV2kwx2sPwJKOR9NRtnRpc/1+wSzS7vGk8ya71C0gMU7f4pf2vwSqNtOME0KSJbR4SjjcFnp8PaxFUmsjP0ijnoE76mBjiWHqiyBJn1j6cBf4bJXJPQlQiZ4F1OlliLFOYk81jM0pQPgwgqF/om/P8+9OKz9a1xIeLBnKkwnKt2dBjhZeZ1tFpruTLdR1LWZjIPOmWKZfV82Qmgcek1O7KtFH4F/x9e8w== } 8, 36 -- X-YMail-OSG: KcDAD2IVM1miC7U6QuOqrAbAE3ZpEChGohU34TtHPElCud1VQEfiO8GR6tBqwW0 } 8, 36 -- WqHRIUFFUqKg6sXLtvXKj7d0PS7vHc41I8dv1SCKucrTUKw7RpEJLkcussukkz9yd.b0.uvFE9xs } 8, 36 -- FfqtjLBqgAV0mh4QmDfJI.quhjfONSWBwUL30JlVTe.EqrE.llX75rjZ.Je8vC7uJWbwlXo3VzjG } 8, 36 -- pbzdcFWDSous2wcm9RfCGRJBEL9HGHDSTBNQ2bOcOXsGAr_mpacd0kmX9CpOiP910On_xWwh_37y } 8, 36 -- lkOPBXDHES_X.MLBsNz8m1JxPj4vRM_dG1JgiOCbVyePA2V78i2htZvBdJ4Yfoxfn5EBg_4TdfZ4 } 8, 36 -- sgUy8IbcjKGCGKjjzSKihRM6D.QMwbZB9GruGnIa4rUWfU6Wv18eKYfBYHvhufSyCcnp4WESYX6B } 8, 36 -- tstgB93l.t8f2E9XV2XZHkEF980bP3X6oS1h5zZ_0EqPPdynJPFdMRCz7yPyJl4f3zgcNB3Zd1XM } 8, 36 -- 08yETtbZoTjTA4eGqP8RLf2R5.FKfsc02UPriDFjnw3GURjL7zbDdvSUe5RWp8PXhTQ_0ztmZS0Q } 8, 36 -- taC2HKOh2369e6kypne13SBNyG45YVie63HGXsuUl6bzgltnS7zfvnFIRxTDMJlksEt.WhMXPgxU } 8, 36 -- EUE7d9XL9UBj1ed.JkvAvHGsZF8CS8kkJz5.JBFxXglEYAe.7kWpvhEc0jkOYRzpJmePAXNS.ivY } 8, 36 -- t_bLjEMwTKgnDLL9Z0F9f_t0IvpA7iYCaw2RZdARCcBLK1p3gtipfMinYe1O2xI7R1NRSJ4g9C_L } 8, 36 -- wBpY6G4I4bAzKaDvourEDIO.7U8_Htci6d1bPkl7IUB6btxD6coaH8yg5csJyjSGtzwJf7Z4jtUt } 8, 36 -- 7d_m.e5hGkqaNHTxM8531QqFJ68A6yH7isf69J3I37mlUp.di8feQpIZtGn0sFpZ.N9dY882OkA1 } 8, 36 -- LA0nDRw4aivOyeN9XTIeMQqtRv_kBmInKN8JV2r2axc_6qQC62IdNmFGYh_E4J5Lm4IPxm_7Zaa6 } 8, 36 -- a6eKuKdyvm1vEvF0VClCeTbqLuGVyX06wLxvv4g7rivJpxabKL2.ajnTDx_Cbf90inh7_LmUMQ4M } 8, 36 -- xlNjyk7kLSUwn_g0vrhNaliH_hUzDaoNgHdX8_ESQKmnOBzHA4KC250WyeRW3n4oSZSYkzKC0Isg } 8, 36 -- 7agAb9UJc_VkXWiqhKYVFOfAvaIq3he7w7iXAvn2bqgQkgPSsyPo29MEgXs9q88zmDKKAZf.GLxp } 8, 36 -- FKLHhT8dKDw2131DHpN4TwheOxUpohl9VWngZ6XU480qtLJrRt8IYkj9t7jiGauPcf.BABJPTm2w } 8, 36 -- 2fsHZFAYz9cs.QwKT6xG9viLb6vEcZA-- } 8, 36 -- Received: from sonic.gate.mail.ne1.yahoo.com by sonic306.consmr.mail.gq1.yahoo.com with HTTP; Wed, 19 Feb 2020 21:38:09 +0000 } 8, 36 -- Date: Wed, 19 Feb 2020 21:36:07 +0000 (UTC) } 8, 36 -- From: Stephane Nizet {nizets2-at-yahoo.com} } 8, 36 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} } 8, 36 -- Message-ID: {317764846.5589118.1582148167228-at-mail.yahoo.com} } 8, 36 -- Subject: [Filtered] question about EDS in SEM } 8, 36 -- MIME-Version: 1.0 } 8, 36 -- Content-Type: text/plain; charset=UTF-8 } 8, 36 -- References: {317764846.5589118.1582148167228.ref-at-mail.yahoo.com} } 8, 36 -- X-Mailer: WebService/1.1.15199 YMailNorrin Mozilla/5.0 (Windows NT 10.0; Win64; x64; rv:72.0) Gecko/20100101 Firefox/72.0 } 8, 36 -- Content-Transfer-Encoding: 8bit } 8, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id 01JMelux001954 } ==============================End of - Headers============================== } }
Let me be sure I understand your question correctly. Your first option of a 30-mm2 detector would allow a 10-mm working distance. Your second option of a 150mm2 detector would require a 15-mm working distance to the pole piece. That does not say anything about the sample to detector distance which is required to calculate the solid angle. The 15-mm working distance might be your better option. That is similar to the situation we ran into. I wished I had posed the question to the list 9 years ago. - We had been using a 10mm2 detector on a Hitachi 2460N which was setup for a 25-mm working distance for the eucentric height and analytical distance. We were able to bring the EDS detector in below the pole piece so that it was quite close to the sample and gave us a pretty good solid angle. It did mean that the EDS detector was the first thing in harm's way. A user scratched up one of our aluminum sample platens when they raised it into the end of the detector then proceeded to move the sample around. - We upgraded to a FEI Quanta with field emission. It's eucentric height was 10-mm which was better for imaging. We knew we wanted to get a large detector in order to maintain as much image resolution as possible when doing EDS so we opted for an 80-mm2 detector figuring we would get 8x more counts at the same beam current. It turned out that we only got about 3x more. We had ordered the system configured to work at 10-mm working distance. With the design of the pole piece, that meant our detector had to stand about 70% further off than before. So we gained 8x from the detector area but lost almost 3x due to the greater distance. I have wondered about redoing our system to operate at 15-mm working distance and bring the detector in much closer. It would greatly improve our solid angle. If I was only doing EDS, I would probably go for it. It would mean different operating conditions for EDS versus just imaging. That might confuse some of our many users. We also have WDS installed, and I understand it is aimed at 10-mm. So, I will probably just leave things as they are and bring up the current a bit more. Now to the other question of performance and resolution as a function of size. It is true that all other things being equal, you may give up a little energy resolution by going to a bigger detector. It is harder to find a crystal of the same, good resolution when it has to be 8x larger, but it can be done. They will probably charge a premium for it. You probably want to compare spectra on your real samples. I choose to back off on the process time to push more counts at the same deadtime. So, I am already sacrificing some resolution. I am glad that I started with a better detector. There are times that I want for better resolution so I increase the process time and cut the count rate - or I switch over to the WDS. Warren Straszheim Materials Analysis and Research Lab Iowa State University
-----Original Message----- X-from: nizets2-at-yahoo.com {nizets2-at-yahoo.com} Sent: Wednesday, February 19, 2020 4:41 PM To: Straszheim, Warren E [BIOTC] {wesaia-at-iastate.edu}
Dear colleagues,
I'd have a simple question regarding the detector area of EDX (EDS) systems in SEM: Clearly the largest the detector, the highest the sensitivity for everything else equal but what if everything else was NOT equal?
One clear example:
- detector 1: 30mm², optimal distance to pole piece 10mm - detector 2: 150mm², optimal distance to pole piece 15mm
Here the largest detector is also the furthest!
I don't know how to appreciate the influence of the 2 factors, detector area and work distance, on the final sensitivity of the system. In other words, is the extra $$$ for the larger detector worth it if the work distance is at the same time increased?
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Title-Subject: [Filtered] Avoiding nanoparticle agglomeration in TEM sample preparation
Message: Hello,
I synthesize 20 nm sized individual amorphous carbon spheres/dots. I use TEM to check the morphology/size distribution of the sample i.e. individual particles or agglomerates. I have prepared the samples by dipping the TEM grid in a tip sonicated water/Ethanol/carbon dot suspension.
I am aware that due to drying of the solvent from the TEM grid, the carbon dots that are present in the drop are forced together and may form agglomerates. I would like to eliminate/minimize the agglomerate formation in the sample preparation process as it is influencing my analysis which is used to evaluate the synthesis method (formation of single particles/formation of agglomerates).
What are the possible methods I could use in my sample prep, to get the least possible particle agglomeration?
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We searched and after several years the answer was clearly no. We settled our traceable TEM calibration problem by calibrating with a replica grating and then averaging the measurement of 200 particles of a NIST traceable nanospheres. We selected the 200nm (actually203nm) traceable spheres and typically get numbers within 5%. There are smaller traceable spheres, but the 200 have the smallest Std deviation and expanded uncertainty,
Please let me know if you find a better solution.
Stay safe..
Frank Karl Microscopist Akron Rubber Development Laboratory 2887 Gilchrist Road Akron, Ohio 44305
Orignal Message:
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X-from: Erico Freitas {ericotadeu-at-ufmg.br}
Dear all,
We are looking after a traceable standard specimen for TEM calibration. We had a MAG*I*CAL sample that got damaged, and it is in a temporary backorder at the EMSDiasum website.
Does any of you know and have used another traceable standard specimen for TEM calibration?
Thank you,
-- Erico Freitas
Physicist/Microscopist at Center of Microscopy Universidade Federal de Minas Gerais (UFMG) Av. Antnio Carlos, 6627, Campus Pampulha, Belo Horizonte, MG, Brazil. ZIP Code 31270-901. +55-31-3409-7573 +55-31-3409-7575
Coordinator:Transmission Electron Microscopy laboratory
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Title-Subject: [Filtered] Avoiding nanoparticle agglomeration in TEM sample preparation
Message: Hello,
I synthesize 20 nm sized individual amorphous carbon spheres/dots. I use TEM to check the morphology/size distribution of the sample i.e. individual particles or agglomerates. I have prepared the samples by dipping the TEM grid in a tip sonicated water/Ethanol/carbon dot suspension.
I am aware that due to drying of the solvent from the TEM grid, the carbon dots that are present in the drop are forced together and may form agglomerates. I would like to eliminate/minimize the agglomerate formation in the sample preparation process as it is influencing my analysis which is used to evaluate the synthesis method (formation of single particles/formation of agglomerates).
What are the possible methods I could use in my sample prep, to get the least possible particle agglomeration?
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Arunas, I forgot to mention UA is incompatible with phosphate and will precipitate in its presence. If you use it then briefly rinse in DH2O before the negative staining (after sample adsorbtion). Good Luck, Michael Delannoy Johns Hopkins SOM Microscope Facility
Dear Arunas, You probably already considered this, but whatever you see on TEM is not going to be representative of what's happening in solution, irrespective of preparation method. (Except, maybe freezing/Cryo_TEM?). If what you want to know is how they are dispersed in solution, then DLS or a similar light scattering technique might be best. 20nm particles are well within the size range that's possible. Although, I suppose there will be relatively little contrast due to the material, it should be feasible. In any case, it's a very quick method to try. Good luck, Pete. ____________________________________________________________________________ Atomic Force Microscopy Dr Peter Eaton and Dr Paul West Available through all good bookshops, or direct from Oxford University Press at: http://ukcatalogue.oup.com/product/9780199570454.do http://afmhelp.com
________________________________________ X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: 21 February 2020 22:25 To: petereaton-at-hotmail.com
To: Zaluzec-at-microscopy.com
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Title-Subject: [Filtered] Avoiding nanoparticle agglomeration in TEM sample preparation
Message: Hello,
I synthesize 20 nm sized individual amorphous carbon spheres/dots. I use TEM to check the morphology/size distribution of the sample i.e. individual particles or agglomerates. I have prepared the samples by dipping the TEM grid in a tip sonicated water/Ethanol/carbon dot suspension.
I am aware that due to drying of the solvent from the TEM grid, the carbon dots that are present in the drop are forced together and may form agglomerates. I would like to eliminate/minimize the agglomerate formation in the sample preparation process as it is influencing my analysis which is used to evaluate the synthesis method (formation of single particles/formation of agglomerates).
What are the possible methods I could use in my sample prep, to get the least possible particle agglomeration?
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Look up "Ammonium Laurate Surfactant for Cleaner Deposition of Carbon Nanotubes" by Hannah Nisson, DOI:10.1021/acs.langmuir.5b01175 on using surfactants to avoid agglomeration during TEM sample prep.
Valery Ray (also with REFINE Lab, UCONN) ========================================== MEO Engineering Company, Inc. DBA "PBS&T" 290 Broadway, Suite 298, Methuen MA 01844 www.partbeamsystech.com www.freudlabs.com E-mail: vray-at-partbeamsystech.com Phone: +1-978-305-0479
On 2/26/2020 6:21 AM, petereaton-at-hotmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } Dear Arunas, } You probably already considered this, but whatever you see on TEM is not going to be representative of what's happening in solution, irrespective of preparation method. (Except, maybe freezing/Cryo_TEM?). If what you want to know is how they are dispersed in solution, then DLS or a similar light scattering technique might be best. 20nm particles are well within the size range that's possible. Although, I suppose there will be relatively little contrast due to the material, it should be feasible. In any case, it's a very quick method to try. } Good luck, } Pete. } ____________________________________________________________________________ } Atomic Force Microscopy } Dr Peter Eaton and Dr Paul West } Available through all good bookshops, or direct from Oxford University Press at: } http://ukcatalogue.oup.com/product/9780199570454.do http://afmhelp.com } } } ________________________________________ } X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} } Sent: 21 February 2020 22:25 } To: petereaton-at-hotmail.com } Subject: [Microscopy] viaWWW:Avoiding nanoparticle agglomeration in TEM sample preparation } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } To: Zaluzec-at-microscopy.com } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy to both arunas.mesceriakovas-at-uef.fi, } Microscopy-at-Microscopy.com so that all Microscopy Listserver } Subscribers can benefit from our collective wisdom } --------------------------------------------------------------------------- } } Email: arunas.mesceriakovas-at-uef.fi Name: Arûnas Meðèeriakovas } } Organization: University of Eastern Finland } } Title-Subject: [Filtered] Avoiding nanoparticle agglomeration in TEM } sample preparation } } Message: Hello, } } } I synthesize 20 nm sized individual amorphous carbon spheres/dots. I use } TEM to check the morphology/size distribution of the sample i.e. } individual particles or agglomerates. I have prepared the samples by } dipping the TEM grid in a tip sonicated water/Ethanol/carbon dot suspension. } } I am aware that due to drying of the solvent from the TEM grid, the } carbon dots that are present in the drop are forced together and may } form agglomerates. } I would like to eliminate/minimize the agglomerate formation in the } sample preparation process as it is influencing my analysis which is } used to evaluate the synthesis method (formation of single } particles/formation of agglomerates). } } What are the possible methods I could use in my sample prep, to get the } least possible particle agglomeration? } } Login Host: 193.167.228.180 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 13, 53 -- From microscopy.listserver-at-gmail.com Fri Feb 21 16:24:33 2020 } 13, 53 -- Received: from mail-pf1-f180.google.com (mail-pf1-f180.google.com [209.85.210.180]) } 13, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 01LMOXxh006217 } 13, 53 -- for {microscopy-at-microscopy.com} ; Fri, 21 Feb 2020 16:24:33 -0600 } 13, 53 -- Received: by mail-pf1-f180.google.com with SMTP id s1so1901822pfh.10 } 13, 53 -- for {microscopy-at-microscopy.com} ; Fri, 21 Feb 2020 13:22:01 -0800 (PST) } 13, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 13, 53 -- d=gmail.com; s=20161025; } 13, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 13, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 13, 53 -- bh=AgZ7Q+ThYg4tKsmX2OzO9C21VnfQI3XQrXOjquhXKf4=; } 13, 53 -- b=tfDlZvElkm1nR5T/H3gSOFp+QE4BcIJqpNhyz6EuUROV7Hd923chs2aBLRLiRGkf3z } 13, 53 -- 4dWWb+gEidcOIGibW0h7mn7SPOGFlv0IRN+SSynCMwYnKdvEy2K0mEpvobIgdR4mLC2E } 13, 53 -- Hg16QjO5o8JCEhxp/ku8doudfv7bBcX1jPW8KYG6xZr7gxIuju/y6M4MIOADq1vcPDDo } 13, 53 -- 292LRPqe3hJUtUuIUMaddy1+5U3b6LtX+CGA/a4xprCZKtJWRiA8MhvsRwuX1wW6atZj } 13, 53 -- JaHHZzJz4YjifwSqzv3VaucCZZz9GaraADYB9J00Ip+3x3Bb7mkAvRzwOxAdi7QFIxfO } 13, 53 -- 5fJw== } 13, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 13, 53 -- d=1e100.net; s=20161025; } 13, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 13, 53 -- :user-agent:mime-version:in-reply-to:content-language } 13, 53 -- :content-transfer-encoding; } 13, 53 -- bh=AgZ7Q+ThYg4tKsmX2OzO9C21VnfQI3XQrXOjquhXKf4=; } 13, 53 -- b=GWWoctZ1bNfLY1sDyUEt2rWcVG4WW6B2HND40eHRLns4A6mLuiOZ3QdBUQFyUMmgva } 13, 53 -- q0yL05igk6Ql6oJlfpPwFYLYTr+aHZ2gZUCs5Ys9V+OloSKg9sbfHcLgMF1xezfVGqh3 } 13, 53 -- Ni/F3t+ynoIRSdCD+a2gP/jhMiPm0Lc7xTOKXd4ZTxEmQhrfA8fMnuiWWDkxF0ZWGsy1 } 13, 53 -- pdxXXCzGWpEWgbz+VimO62zMKX1835MZ8/xfynprOVMpFGg1ITe607Ii+LqZfySH0cWR } 13, 53 -- ihl+I/Uw53VK9FVQkPRmMsa9lzY3/FCmVUtHTZqN3YJLlSUQfwRoNJg6OLJkfIO8FzkU } 13, 53 -- csLA== } 13, 53 -- X-Gm-Message-State: APjAAAVqzkTe8GFWvX0sVLi0a6oj0w6NvQcd1DEY5seUy+11wAmmElCF } 13, 53 -- CJ4yUOzIroWyi144dCd4uE4H0RUZ } 13, 53 -- X-Google-Smtp-Source: APXvYqzLwQobnTnTS8ReSVCwYYHOccwU6ygcXmaQgYUXoZQRRjLVc9FFlSw9E3LyWGkw+2thRMQxKg== } 13, 53 -- X-Received: by 2002:a62:8e0a:: with SMTP id k10mr40655825pfe.49.1582320120226; } 13, 53 -- Fri, 21 Feb 2020 13:22:00 -0800 (PST) } 13, 53 -- Received: from Nestor-MacBookAir-Pro-2014-ElCapitan.local (ppp59-167-172-94.static.internode.on.net. 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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both hancocksk-at-vt.edu, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Email: hancocksk-at-vt.edu
Name: Sandy Hancock
Organization: College of Veterinary Medicine, Virginia Tech
Title-Subject: [Filtered] EM Lab Manager position at CVM Virginia Tech
Message: Hello EM Colleagues,
The Electron Microscopy Laboratory at the College of Veterinary Medicine at Virginia Tech has a position open for a Lab Manager. The position is responsible for the day-to-day operations of the service laboratory, which primarily supports researchers on campus who submit biological samples, with the occasional nanoparticle/materials sample. The laboratory houses a JEOL JEM-1400 TEM, Zeiss EVO 40 SEM and supporting equipment for specimen preparation. More information can be found at the position listing http://careers.pageuppeople.com/968/cw/en-us/job/512843/electron-microscopy-lab-mgr
Thank you! Sandy
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Email: ralvaradojr-at-ufl.edu Name: Rudy Organization: University of Florida
Title-Subject: [Filtered] Buffers other than PBS to use for IEM
Message: Hi everyone, I am trying to process a strain of Lactobacillus johnsonii bacterial cells for immunoelectron microscopy. These cells hate PBS. My question is: is there other buffers I can use to process this sample that don't interfere with antigenicity? Thanks Login Host: 128.227.146.124 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
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On Fri, Feb 28, 2020 at 9:38 AM {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote:
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Email: ralvaradojr-at-ufl.edu {mailto:ralvaradojr-at-ufl.edu} Name: Rudy Organization: University of Florida
Title-Subject: [Filtered] Buffers other than PBS to use for IEM
Message: Hi everyone, I am trying to process a strain of Lactobacillus johnsonii bacterial cells for immunoelectron microscopy. These cells hate PBS. My question is: is there other buffers I can use to process this sample that don't interfere with antigenicity? Thanks Login Host: 128.227.146.124 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
********************************************** Geoff McAuliffe, Ph.D. Assistant Professor Director, Electron Microscopy Core Department of Neuroscience and Cell Biology Rutgers, Robert Wood Johnson Medical School 675 Hoes Lane West, Piscataway, NJ 08854 voice: (732) 235-4583 mcauliff-at-rwjms.rutgers.edu **********************************************
I suggest to process in a buffer that is compatible with your bacterial strain. Buffers in general will not have a negative effect on antigenicity. I mainly used 100mM PB and PHEM buffers in the past, when processing cells in culture.
Are you going to chemically fix your specimen with aldehydes? Do you intend to embed in a plastic? Choose/work out a fixation method and embedding method compatible with the characteristics of the primary antibody. You may test the effect of fixation on signal intensity already on the LM level using an immunofluorescent method or combine immunogold labeling with silver enhancement.
During the actual immuno incubation it will be beneficial to use a buffer with sodium chloride. In general PBS, pH 7.4 is a good option. TBS is an alternative. The buffer capacity of Tris at pH 7.4 is however suboptimal. You may go up to a higher pH, e.g., pH 8.2 depending on the additional additives you use to diminish background staining. I recommend https://aurion.nl/sharing-our-knowledge/newsletters-and-newsflyers/newsletter-1-background-suppression/for additional information.
Kind regards,
Peter van de Plas
} On 28 Feb 2020, at 01:39, microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } X-from: shakeel waqqar {shakeelwaqqar-at-gmail.com {mailto:shakeelwaqqar-at-gmail.com} } } } } Try Normal Saline } } On Fri, Feb 28, 2020 at 9:38 AM {microscopy.listserver-at-gmail.com } {mailto:microscopy.listserver-at-gmail.com} } {mailto:microscopy.listserver-at-gmail.com} } wrote: } } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line {http://www.microscopy.com/MicroscopyListserverOn-Line} Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } X-from: ralvaradojr-at-ufl.edu {mailto:ralvaradojr-at-ufl.edu} {mailto:ralvaradojr-at-ufl.edu} } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy to both ralvaradojr-at-ufl.edu {mailto:ralvaradojr-at-ufl.edu} } {mailto:ralvaradojr-at-ufl.edu} , } Microscopy-at-Microscopy.com {mailto:Microscopy-at-Microscopy.com} so that all Microscopy Listserver } Subscribers can benefit from our collective wisdom } } --------------------------------------------------------------------------- } } Email: ralvaradojr-at-ufl.edu {mailto:ralvaradojr-at-ufl.edu} {mailto:ralvaradojr-at-ufl.edu} } Name: Rudy } Organization: University of Florida } } Title-Subject: [Filtered] Buffers other than PBS to use for IEM } } Message: Hi everyone, } I am trying to process a strain of Lactobacillus johnsonii bacterial } cells for immunoelectron microscopy. These cells hate PBS. 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Peter van de Plas Aurion Binnenhaven 5 Wageningen 6709 PD The Netherlands Phone: +31 317 415094 http://www.aurion.nl
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Email: rvancamp-at-kettering.edu
Name: Rick Van Camp
Organization: Kettering University
Title-Subject: [Filtered] Printing tiff images
Message: I would like to display some of the tiff images that have been collected with our TEM in the hall outside the lab. Can anyone advise me on what properties a given printer needs to possess such that these images retain the high quality they inherently possess? The idea is to leave a positive impression on those people that walk by the lab.
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Dear Rick, Unfortunately I don’t have practical experience with PINTING TIFF files properly…don’t own a TIFF-printable printer (☺)
But: Information is available: e.g. https://itstillworks.com/print-large-tiff-file-6469452.html
Copy & Paste: Just for convenience of all Readers/colleagues eventually not being able to access the given URL: QUOTE: How to Print a Large Tiff File Step 1 Select a paper that is suited to print photographic images. Using plain typing paper or copy paper will not capture the range of values and colors inherent in a high-quality Tiff file. If possible, select the same type of paper as the manufacturer of your printer. Step 2 Access a printer that is designed to Tiff quality images, if possible. Most home inkjet printers will print a photographic image of an acceptable quality, though photo-specific printers will give the contrast and color range that a Tiff is designed to provide. These types of printers often have the word "photo" in their model name, and use individual-color ink cartridges. Step 3 Clean the print heads on your printer. This prevents lines, known as banding, as well as color shifts. Right-click on the printer icon in the taskbar, located on the lower right corner of your screen. Follow the step-by-step instructions after clicking the option to clean the print heads. Step 4 Open the Tiff file in your computer's imaging software or default picture viewer. Make any changes to contrast, color and cropping as necessary or possible. Step 5 Select Print from the File menu in the software. Select the option called Page Options or Print Options, depending on your software model. This will bring up a dialog box specific to your printer. Step 6 Choose the paper size, orientation, the source. The size will likely be legal, or 8.5 by 11 inches. The orientation refers to whether the image is tall (portrait) or long (landscape). The source is either a sheet or roll of paper. Step 7 Select the type of media being used. The instruction sheet with your paper may suggest a certain media based on your printer manufacturer. There may be a number of options to experiment with, but the important detail is to select whether the paper you have chosen has a glossy surface or is matte. Step 8 Select the highest print quality available. This option is known under various terms depending on the manufacturer, but will usually be the top or last quality in a list of options. Load the paper into the printer on its correct side as explained by the paper's instruction sheet. Press print. If the print has problems with its contrast or color, return to the file in the imaging software. Make the necessary changes and repeat the process as needed. Tip • So as not to waste ink, use the selector tool in your imaging software to choose a portion of the image with a variety of colors or contrast. Copy and paste this portion into a new document. Make the necessary changes and print test strips until it is determined what changes to apply to the entire image. Items you will need • Computer with imaging software or default picture viewer • Tiff file • Printer, preferably of photographic quality
Elizabeth MOTT, My Printer Is Not Printing TIFF Files https://smallbusiness.chron.com/printer-not-printing-tiff-files-57302.html [ Related Articles, References (3) https://lbis.kenyon.edu/helpline/printers/troubleshooting Microsoft: Description of the Guidelines for Selecting the Appropriate Picture Format in an Office Program Adobe Systems: Developer Resources: TIFF Resources (1) Dux Computer Digest: How to Troubleshoot Printer Problems ] What Is the Best Image Format for Printing? https://www.shutterstock.com/support/article/best-image-format-for-printing
Asked Feb 2019: https://photo.stackexchange.com/questions/104804/jpeg-or-tiff-files-better-for-print-images JPEG or TIFF files better for print images?
Asked August 2007 https://www.photo.net/discuss/threads/is-it-possible-to-print-tiff-files.283228/
Best regards and good luck,
Wolfgang MUSS SALZBURG, AUSTRIA
========================================================= MUSS Wolfgang Dr. phil. / PhD - retired Ignaz-Rieder-Kai 19/6 A-5020 SALZBURG Österreich-AUSTRIA Mobile-Tel.: 0043(0)676 5 369 456 E-mail: wij.muss-at-aon.at E-Mail altern.: womuss-at-gmail.com FRMS, Retired Member of MSA & other (Inter-)National Societies Former Head of Electron Microscopy Lab at Institute of Pathology SALK-LKH / Salzburger Landeskliniken | General Hospital and PMU (private) PARACELSUS MEDICAL UNIVERSITY of SALZBURG Scientific Profile at ResearchGate: http://www.researchgate.net/profile/Wolfgang_MUSS inviting you to join RG (Sign up - ResearchGate -at- https://www.researchgate.net/signup.SignUp.html, and join 15+ million researchers, including 63 Nobel Laureates) „ResearchGate is the professional network for scientists and researchers. Over 15 million members from all over the world use it to share, discover, and discuss research. We're guided by our mission to connect the world of science and make research open to all.“
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If you are interested in printing out your images with your printer. Make sure that your printer has the capabilities to print on photo paper and what type of ink it uses. If you check the name/version of your printer you can look up to see what your printer can and cannot do for printing qualities. When you do print be sure to make sure that the file format/ size of image you have. Due to when you print out your image and you manipulate it may warp the image. Here are some links to help you. -Julie
Link best printer for photos } } https://www.bestproducts.com/tech/electronics/a26933257/top-rated-photo-printers/?src=arb_ga_bp_m_bm_a26933257&utm_source=google&utm_medium=cpc&utm_campaign=arb_ga_bp_m_bm_a26933257&gclid=EAIaIQobChMIgPyip-CE6AIVkspkCh1Mowy8EAAYASAAEgJGa_D_BwE
Why do pictures look different when I print them link } } https://www.google.com/amp/s/www.howtogeek.com/397798/why-do-photos-look-different-when-i-print-them/amp/
Sent from my iPhone
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Can anyone advise me on what properties a given printer needs to possess } such that these images retain the high quality they inherently possess? 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1) Epson Photo printer with Photo inks, 6 to 9 color (the blue ink is used in B&W printing). But! Don't buy one unless you will be printing at least once a month, maybe even once a week. Inks are hideously expensive and 3rd party inks don't work well or at all (inks are bar coded so a printer company can prevent other brands or refills from being used). However. Unless you do print something, even a test page, every so often, the ink dries, clogs a valve or three, and this cannot be fixed. The printer is trash. So, if you just want a set of images for the hallway, use the campus printing service or a business.
2) High quality photo paper. Let the ink completely dry (it sits on top of the coating, hence higher resolution printing) for 30-60 minutes. *Laminate* the print. This seals out oxygen, which fades images.
3) When moving the image files, do *not* use drag-and-drop, use copy/paste. Drag and drop in Windows can change the image resolution to the monitor's resolution. This was true, I do not know if it is still true with Windows 10 (or 7), but I don't trust it. I don't know about Linux, and I don't think MacOS does this. But copy/paste is easy.
4) Not what you asked, but more of a tech-looking thing that makes admin types happy: Put up a display monitor or three in the hallway, and set up a computer to send images to the monitors. No printer, no buying expensive inks, no worrying about file types and sizes, easier to change images, etc. You can maybe get the monitors and computer from campus IT - ones that have been replaced with something newer, but are still perfectly good and likely free.
Phil ------------- Philip Oshel Imaging Facility Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 989 774-3576 office
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We have a job vacancy for a permanent position, part time, technician in our microscopy/microanalysis laboratory here at AgResearch Institute in New Zealand.
We are primarily working on biological and bio-based materials using SEM, TEM and other similar methods. We are looking to hire a technician to carry out a range of tasks from assisting with sample preparation to helping look after the instrumentation and do some troubleshooting etc. The role would suit someone with a good working knowledge with instrumentation, an eye for detail and good collaboration skills.
For more details look under AgResearch on the Science New Zealand website under careers for more details.
*AgResearch Limited | *Lincoln Research Centre *| New Zealand* Dr Duane P Harland, Senior Scientist *T*+64 3 321 8710 *E*duane.harland-at-agresearch.co.nz {mailto:duane.harland-at-agresearch.co.nz}
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Title-Subject: [Filtered] We are hiring! Electron Microscopist / Imaging Specialist
Message: Hello! Sivananthan Laboratories, Inc. is a high-tech business incubator focused on promoting economic growth in Illinois and the United States through fostering cutting-edge, fundamental research and development. We work on R&D for AI, infrared detectors, image processing, solar energy, and more. We are currently looking for a successful candidate who is skilled in all aspects on scanning electron microscopy (SEM), transmission electron microscopy (TEM) and scanning transmission electron microscopy (STEM) with experience in image simulation and state-of-the-art processing and analytics packages. If you are interested, please go to this link (http://sivananthanlabs.us/employment/) and apply for this position!
If you have any questions, feel free to email me at mrajasingham-at-sivananthanlabs.us
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One of my users wants to replace a tungsten filament with aLaB6tip in my FEI BioTwin12 for screening/imaging cryoEM samples. I know aLaB6is much more expensive. But regardless the cost, what are other downside impact on my other users who primarily use the scope for imaging plastic sections, negative-stained samples? Thank you!
We're about to the same in our Tecnai Spirit Biotwin T12. The FEI/Thermofisher guys have no concern about that.
I've used a T12 in CMM at the University of Queensland, Brisbane/Australia that has got a LaB6.
You must clean the gun assembly and the Wehnelt as well in order to get rid of W residues before.
Although a LaB6 is more expensive than a W filament, its life time is much longer. It might last over 1000 ours. It lasts 2000 hours in average on our Tecnai T20. You know it will give you a brighter beam, and its energy spread is lower.
Regarding the other users, they will be quite happy when looking at their plastic sections and negative staining, not only the cryo-EM users.
It's always a good practice to set a lower C2 aperture and play around with the spot size to a suitable electron dose for each sample. I know some users don't like to change the apertures and do the alignments but they should get use of it.
My only concern is about the gun preassure. As the Tecnai Spirit Biotwin T12 does have a dedicated IGP for the gun (ours doesn't have), the vacuum in the gun is broken down every time you run the cryo cycle. So the LaB6 cathode gets contaminated. But I still think the benefits of the LaB6 will give you are worth to have it on your T12.
Best wishes,
On Sat, 7 Mar 2020, 16:56 , {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote:
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X-from: Ning, Gang {gxn7-at-psu.edu {mailto:gxn7-at-psu.edu} }
Dear Listers,
One of my users wants to replace a tungsten filament with aLaB6tip in my FEI BioTwin12 for screening/imaging cryoEM samples. I know aLaB6is much more expensive. But regardless the cost, what are other downside impact on my other users who primarily use the scope for imaging plastic sections, negative-stained samples? Thank you!
if your FEI TEM is equipped with a getter pump and does reach a sufficient vacuum value you will generally benefit from switching to LaB6 cathodes. An LaB6 cathode can be pre-mounted in a spare wehnelt and interchanged within an hour, if the vacuum system in the rest of the scope is pumped down to limit. Use dry Nitrogen gas for flooding and let it run until cathode / wehnelt is changed... LaB6 cathode needs to be centred VERY well, within a few 1/100 mm. For accurate height settings see the data sheets at Kimball or DENKA websites.
The spot diameter will be smaller than with Tungsten, the electrons coming from the cathode more monochromatic. Both things will help getting better resolution / better focus and astigmatism corrections in the images.
The luminosity of the gun will be significantly higher; that would help at magnifications more than 50-100 k... But: very thin UM cuts could burn under a concentrated beam.
I suppose the automatic heating regime for the LaB6 cathode could be selected at the TEM setup. It will need slow heating (and cooling), ca. 2 minutes each.
Other than the cathode handling and the costs (but: cathode will run ca. 1000-2000 hours) there is only win-win...
I use LaB6 since a lot of years at a Philips / FEI EM420.
Best,
Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
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Email: jpshield-at-uga.edu Name: John Shields
Organization: University of Georgia
Title-Subject: [Filtered] lead aspartate as post-stain
Message: Hello everyone, I have been searching for any work on lead aspartate used as a post-stain (similar to lead citrate). Unfortunately I only pull up its use as an en bloc stain - lately for serial section SEM sample prep. If anyone has literature, or has done some previous work seeing if LA would work, I would appreciate any and all information.
THanks! John Shields Univ. of Georgia
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X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu}
John,
Lead aspartate? Isn't that used as a sweetner in some of the heavier diet pastries?
Phil ----------------------------------------- Philip Oshel Imaging Center Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576 office (989) 774-7567 lab
________________________________________ X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Monday, March 9, 2020 16:08 To: Oshel, Philip Eugene
X-from: jpshield-at-uga.edu
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Email: jpshield-at-uga.edu Name: John Shields
Organization: University of Georgia
Title-Subject: [Filtered] lead aspartate as post-stain
Message: Hello everyone, I have been searching for any work on lead aspartate used as a post-stain (similar to lead citrate). Unfortunately I only pull up its use as an en bloc stain - lately for serial section SEM sample prep. If anyone has literature, or has done some previous work seeing if LA would work, I would appreciate any and all information.
THanks! John Shields Univ. of Georgia
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No no no, you're think of sugar of lead or lead acetate. Hey, it worked for the Roman Empire.
Stay safe...........
Frank Karl Microscopist Akron Rubber Development Laboratory 2887 Gilchrist Road Akron, Ohio 44305
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Monday, March 9, 2020 7:19 PM To: Frank Karl {frank_karl-at-ardl.com}
X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu}
John,
Lead aspartate? Isn't that used as a sweetner in some of the heavier diet pastries?
Phil ----------------------------------------- Philip Oshel Imaging Center Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576 office (989) 774-7567 lab
________________________________________ X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Monday, March 9, 2020 16:08 To: Oshel, Philip Eugene
X-from: jpshield-at-uga.edu
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Email: jpshield-at-uga.edu Name: John Shields
Organization: University of Georgia
Title-Subject: [Filtered] lead aspartate as post-stain
Message: Hello everyone, I have been searching for any work on lead aspartate used as a post-stain (similar to lead citrate). Unfortunately I only pull up its use as an en bloc stain - lately for serial section SEM sample prep. If anyone has literature, or has done some previous work seeing if LA would work, I would appreciate any and all information.
THanks! John Shields Univ. of Georgia
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Oh Right, right, Sorry, I mean the "Stain formerly known as Lead Aspartame". Sheesh John S
On Wed, Mar 11, 2020 at 9:04 AM {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote:
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X-from: Frank Karl {frank_karl-at-ardl.com {mailto:frank_karl-at-ardl.com} }
No no no, you're think of sugar of lead or lead acetate. Hey, it worked for the Roman Empire.
Stay safe...........
Frank Karl Microscopist Akron Rubber Development Laboratory 2887 Gilchrist Road Akron, Ohio 44305
-----Original Message----- X-from: microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} [mailto:microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} ] Sent: Monday, March 9, 2020 7:19 PM To: Frank Karl {frank_karl-at-ardl.com {mailto:frank_karl-at-ardl.com} } Subject: [Microscopy] Fwd: Re: viaWWW: lead aspartate as post-stain
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X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu {mailto:oshel1pe-at-cmich.edu} }
John,
Lead aspartate? Isn't that used as a sweetner in some of the heavier diet pastries?
Phil ----------------------------------------- Philip Oshel Imaging Center Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576 office (989) 774-7567 lab
________________________________________ X-from: microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } Sent: Monday, March 9, 2020 16:08 To: Oshel, Philip Eugene Subject: [Microscopy] viaWWW: lead aspartate as post-stain
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Email: jpshield-at-uga.edu {mailto:jpshield-at-uga.edu} Name: John Shields
Organization: University of Georgia
Title-Subject: [Filtered] lead aspartate as post-stain
Message: Hello everyone, I have been searching for any work on lead aspartate used as a post-stain (similar to lead citrate). Unfortunately I only pull up its use as an en bloc stain - lately for serial section SEM sample prep. If anyone has literature, or has done some previous work seeing if LA would work, I would appreciate any and all information.
THanks! John Shields Univ. of Georgia
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Email: mbrukman-at-seas.upenn.edu Name: Matthew Brukman
Organization: University of Pennsylvania
Title-Subject: [Filtered] High Vacuum STM available
Message: Good morning!
We have a surplus high vacuum STM system free to a good home. Components include
RHK controller Omicron STM-1 head vacuum chamber PC with RHK software roughing, turbo, and ion pumps frame with air-supported legs
Recipient would be responsible for transport and reassembly. Please contact me with questions.
Regards, Matt
Matthew Brukman Scanning and Local Probe Facilities Director Singh Center for Nanotechnology 3205 Walnut St. Phila., PA 19104 215-746-2373
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Is there a recommended procedure /materials for disinfecting optical microscope eyepieces, optics and surfaces against COVID-19?
Alan Paris Leica Microsystems Inc. alan.paris-at-leica-microsystems.com
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Email: nmz787-at-gmail.com Name: Nathan McCorkle
Organization: Sole proprietor
Title-Subject: [Filtered] How to clean nickel shim of magnetic and or glass particles?
Message: I have a nickel shim destined for nanoimprint lithography, made by electroforming e-beam exposed photoresist. I don't have a proper cleanroom, but I've been trying to strip what seemed like residual ZEP e-beam resist... And it has not been going so well. I've tried acetone, dichloromethane, n-methyl pyrrolidone, and 10% NaOH. Sonicated with heat in both acetone and NaOH (at different times). The NaOH is the most recent attempt, and it seemed to show improvement under FIB imaging, but I also noticed what appeared to be redeposition. I can only imagine this is due to particulate in my solvents, dirty air as I blow dry the shim or carry it from my sonicator to my FIB desk, or maybe insoluble particles like glass or ferromagnetic dust which start to settle onto the sample as soon as the sonicator is turned off.
Features are around 150nm linewidth, high frequency and complex shaped... So lots of small say 500nm sized holes/crevices which I'd been thinking was just diffusion limited for the solvent to get into and do it's work. But now I'm pretty confused.
Should I invest in some .45 and .22 micron syringe filters for all my fluid work? Should I tape a magnet to the outside of the beaker I've been sonicating in, to try and collect such particles? What's a standard semiconductor lab method for cleaning magnetic particles from magnetic layers? How about the idea of insolubles? Or can someone recommend a solution that will etch glass but not nickel?
Thanks, -Nathan
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Research Scientist Materials Research Laboratory University of Illinois at Urbana-Champaign
The Materials Research Laboratory (MRL) at the University of Illinois at Urbana-Champaign is seeking a highly motivated Research Scientist to participate and actively support research in the area of scanning electron microscopy. The Research Scientist will provide comprehensive technical support and user training for equipment, procedures, and safety in the broad areas of materials microanalysis and microfabrication.
The successful candidate will become a member of the dedicated staff of approximately 20 scientists and engineers, who maintain major research instruments in the MRL's core facilities for electron microscopy, soft materials characterization, scanning probe microscopy, laser and optical spectroscopy, surface analysis, x-ray diffraction, and nanofabrication facilities including two cleanrooms. Approximately 1,000 researchers from across our campus as well as other academic institutions, industry, and national laboratories use the facilities, logging more than 100,000 user hours annually. The lab is recognized as one of the premier mid-sized user facilities in the nation. For details, please visit us online at https://mrl.illinois.edu/facilities/.
The University of Illinois is an Equal Opportunity, Affirmative Action employer. Minorities, women, veterans and individuals with disabilities are encouraged to apply. For more information, visit http://go.illinois.edu/EEO.
Responsibilities include: * Prepare and deliver primary and advanced training on the various techniques and instrumentation available in the electron microscopy core, particularly in scanning electron microscopy and related techniques. * Formulate, compile and distribute suitable suggestions for documentation improvements for collective staff scientist review. Incorporate approved modifications into training documents and procedures. This includes the creation of video and multimedia tutorials. * Actively participate in research using electron microscopy techniques in new materials, such as ceramics, metals, semiconductor multilayers and super lattices, polymer and biological materials, etc. * Perform routine preventative maintenance tasks which vary daily, monthly, and annually for assigned laboratory instrumentation. Examples include daily checks to ensure instruments are running correctly; monthly proactive checks to ensure no hazards are present; annual maintenance service on vacuum pumps and supporting mechanical equipment. * Identify hazards and/or potential failure modes by comparing equipment usage and performance to establish safety protocols while conducting user training or performing maintenance. If the equipment is not operating within tolerances or any engineering safety controls are malfunctioning, determine corrective actions to hardware, operating procedures or user training in conjunction with the assigned staff scientist and implement the changes. * Crosstrain in other facility techniques in order to act as backup for other staff members and increase knowledge and experience. * Give oral presentations to large audiences, including recording training material for media outlets, and online, live video streaming. * Perform facility-wide safety tasks as assigned. * Conduct department or campus specified lab inspections for assigned operating areas and participate in reviews of non-assigned areas as requested. * Assume additional responsibilities to promote the unit's mission as needed.
Minimum Qualifications * Ph.D. degree in engineering, chemical, physical sciences or related field. * Two years of hands-on experience in the following areas: * operation of scanning electron microscopes, including detailed knowledge of main physical principles, concepts and applications of electron microscopy; * training researchers in the use of scanning electron microscopes including data interpretation on related techniques; * troubleshooting, preventive maintenance and routine repair of scanning electron microscopes; * use of advanced analytical techniques such as energy-dispersive X-ray spectrometry, cathodoluminescence imaging and spectrometry, and electron backscatter diffraction using the scanning electron microscope. * sample preparation techniques for electron microscopy including polishing, coating, milling, etc. * Three years of instructional/training experience delivering technical information. * Previous experience in creating/developing instructional, instrument operation and training material in electron microscopy. * Excellent oral and written communication skills.
Preferred Qualifications * Experience working in a multi-user academic research facility. * Post-doctoral experience in engineering, chemical, physical sciences or related field. * Chemistry background necessary for identifying chemicals for waste processing. * Experience with operation of focused ion beam (FIB/SEM) systems and transmission electron microscopes and related techniques.
This is a full-time, benefits-eligible academic professional position appointed on a 12-month service basis. The expected start date is as soon as possible. Salary is commensurate with experience and qualifications. To apply, please complete a candidate profile at http://jobs.illinois.edu and upload the following as a single file: a cover letter, resume, and the names and contact information for three professional references. To ensure full consideration, all requested information must be submitted by April 2, 2020.
For further information regarding application procedures, contact Summer Redman at mailto:sredman-at-illinois.edu or 217-300-5400.
The University of Illinois conducts criminal background checks on all job candidates upon acceptance of a contingent offer. As a qualifying federal contractor, the University of Illinois System uses E-Verify to verify employment eligibility. The University of Illinois must also comply with applicable federal export control laws and regulations and, as such, reserves the right to employ restricted party screening procedures for applicants.
________________________________________ James C Mabon, Ph.D. Senior Research Scientist University of Illinois at Urbana-Champaign Materials Research Laboratory 104 S. Goodwin Ave. Urbana, Illinois, 61801 ________________________________________
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Email: Stephen.Beck-at-ncc.edu Name: Steve Beck
Organization: Nassau Community College
Title-Subject: [Filtered] Teaching SEM Online
Message: Dear Colleagues, I am teaching my SEM course this semester and like many institutions, we are trying to teach online/remotely as our campus is closed due to COVID-19. I have administered my midterm exam online, however, after that we need to get on the SEM to image the required samples. Does anyone have any ideas regarding teaching SEM online? I have informed my administration that this is impossible - I haven't received the courtesy of a response yet!
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It is very challenging situation, but everything depends on what generation of hardware you have in the lab. Depends on who is your vendor, it may be possible to share the screens and allow users to move the mouse and type on the screen. Such things exist for the troubleshooting on most of the modern instruments. Technically, some instruments can be even used remotely for a high quality work. The only bottleneck I'd - someone needs to.prepare the sample and mount it inside the analytical tool. As the instruments get more and more sophisticated and automated - that approach may become more popular anyway.
Ask your vendor - they may already have the answer for you.
Stay healthy!
Lolita 8:45 AM, March 21, 2020, "microscopy.listserver-at-gmail.com" {microscopy.listserver-at-gmail.com} :
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Email: Stephen.Beck-at-ncc.edu {mailto:Stephen.Beck-at-ncc.edu} Name: Steve Beck
Organization: Nassau Community College
Title-Subject: [Filtered] Teaching SEM Online
Message: Dear Colleagues, I am teaching my SEM course this semester and like many institutions, we are trying to teach online/remotely as our campus is closed due to COVID-19. I have administered my midterm exam online, however, after that we need to get on the SEM to image the required samples. Does anyone have any ideas regarding teaching SEM online? I have informed my administration that this is impossible - I haven't received the courtesy of a response yet!
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We have a couple of our SEMs connected to the internet for remote access. Through this interface, the microscope is completely controllable from your own PC. We can load your samples and then you or your students would be able to take control of the instrument and practice from your own homes.
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Email: Stephen.Beck-at-ncc.edu {mailto:Stephen.Beck-at-ncc.edu} Name: Steve Beck
Organization: Nassau Community College
Title-Subject: [Filtered] Teaching SEM Online
Message: Dear Colleagues, I am teaching my SEM course this semester and like many institutions, we are trying to teach online/remotely as our campus is closed due to COVID-19. I have administered my midterm exam online, however, after that we need to get on the SEM to image the required samples. Does anyone have any ideas regarding teaching SEM online? I have informed my administration that this is impossible - I haven't received the courtesy of a response yet!
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did you try using a plasma cleaner for cleaning the surfaces and also a plasma cleaner like the Evactron at the FIB chamber to keep the specimen clean during scanning?
If you can mount the specimen with the surface to be cleaned facing down to the bottom of the beaker you might get rid of deposits coming from above ;-)
Another try to clean the surfaces might be to plunge it in liquid nitrogen or to use a vacuum chamber with the cleaning solution and pump to a level below sublimation.
And sure: clean micro-filtered solutions would help.
Nickel and magnetism: you could use a demagnetizer to decrease / erase the magnetism in the shim first.
Best wishes,
Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Am 21.03.20 um 14:55 schrieb microscopy.listserver-at-gmail.com: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: nmz787-at-gmail.com } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy to both } nmz787-at-gmail.com, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can } benefit from our collective wisdom } --------------------------------------------------------------------------- } } Email: nmz787-at-gmail.com Name: Nathan McCorkle } } Organization: Sole proprietor } } Title-Subject: [Filtered] How to clean nickel shim of magnetic and or glass particles? } } Message: I have a nickel shim destined for nanoimprint lithography, made by electroforming e-beam } exposed photoresist. I don't have a proper cleanroom, but I've been trying to strip what seemed like } residual ZEP e-beam resist... And it has not been going so well. I've tried acetone, } dichloromethane, n-methyl pyrrolidone, and 10% NaOH. Sonicated with heat in both acetone and NaOH } (at different times). The NaOH is the most recent attempt, and it seemed to show improvement under } FIB imaging, but I also noticed what appeared to be redeposition. I can only imagine this is due to } particulate in my solvents, dirty air as I blow dry the shim or carry it from my sonicator to my FIB } desk, or maybe insoluble particles like glass or ferromagnetic dust which start to settle onto the } sample as soon as the sonicator is turned off. } } Features are around 150nm linewidth, high frequency and complex shaped... So lots of small say 500nm } sized holes/crevices which I'd been thinking was just diffusion limited for the solvent to get into } and do it's work. But now I'm pretty confused. } } Should I invest in some .45 and .22 micron syringe filters for all my fluid work? Should I tape a } magnet to the outside of the beaker I've been sonicating in, to try and collect such particles? } What's a standard semiconductor lab method for cleaning magnetic particles from magnetic layers? How } about the idea of insolubles? Or can someone recommend a solution that will etch glass but not nickel? } } Thanks, } -Nathan } } Login Host: 50.39.163.117 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } ==============================Original Headers============================== } 11, 53 -- From microscopy.listserver-at-gmail.com Sat Mar 21 08:47:00 2020 } 11, 53 -- Received: from mail-il1-f182.google.com (mail-il1-f182.google.com [209.85.166.182]) } 11, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 02LDl0KO030119 } 11, 53 -- for {microscopy-at-microscopy.com} ; Sat, 21 Mar 2020 08:47:00 -0500 } 11, 53 -- Received: by mail-il1-f182.google.com with SMTP id h3so8517536ils.3 } 11, 53 -- for {microscopy-at-microscopy.com} ; Sat, 21 Mar 2020 05:46:01 -0700 (PDT) } 11, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 11, 53 -- d=gmail.com; s=20161025; } 11, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 11, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 11, 53 -- bh=mH3hQX0JfJ+qEqtaq+MXuNo/ABuFHuYdS+6Lkr4Y6yM=; } 11, 53 -- b=dnnl/o8YK+SszO3Nm5rJjrGFzXDjuoO5012DEK5glVzMak6UpaVSkXllHz30ah4dAv } 11, 53 -- G66sme2fXNthcQ5O0XdjTmWqGVc+8VxfFN2cxlHCFB7a5Y67E3B/w+3vpW6QbV/OwjOZ } 11, 53 -- 17vqHlxRNI9UvrRRGHmLRqdIEmKtMIACWFkjZ7dqR5lH2w8+kgOR/T5RBDcKpXr3InG1 } 11, 53 -- qGJIMRf+jMmpAvUQYDZitQaKgtWxAFM8FdCGKxj796bg0FDI72jIjozC+3N/Go1W+Wnk } 11, 53 -- VnbVRHvSoeU2k0gYmIseWSVfZbh8soOB3Udj+YGWxDkkDOwyfwKKN0lo+N3MmcAISK7A } 11, 53 -- UCIw== } 11, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 11, 53 -- d=1e100.net; s=20161025; } 11, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 11, 53 -- :user-agent:mime-version:in-reply-to:content-language } 11, 53 -- :content-transfer-encoding; } 11, 53 -- bh=mH3hQX0JfJ+qEqtaq+MXuNo/ABuFHuYdS+6Lkr4Y6yM=; } 11, 53 -- b=hXZas+rlSuV0lba6didsrmxBAeXacuYQe7mUNe4DfyHww5h2MmRBFx0D+Sb4ItbEBD } 11, 53 -- joqvretzWGXjPstPy5pGe5fkSGqV17N1rrESsiNMPm3Z0Yuruz973pMxNcA+ReBe6SDQ } 11, 53 -- z69BxT6UV6CU0GkO3YZFe9r9c2b5gsqWlJwpb8JbrqZyh4Zs9zsUwt2ARArCxEhVrCLq } 11, 53 -- 10UrcVCj9xnsslxdB/kPKxC2vQ6J7x6X03H6PJstFULA/WAQdGvjmFnRF0BWIPAyyxY2 } 11, 53 -- EelGn5xXqZWbv5OKiNIaGAasJFtajGL2cwkcwDe8D4BOYFePF48Y3pGZHUKM1S8KxU15 } 11, 53 -- J7RA== } 11, 53 -- X-Gm-Message-State: ANhLgQ0adY4veLh0VKZn/9UFDOB+linnE0u8xD34kP+ALeXDuI6InkR/ } 11, 53 -- lcMX8MnBbO+Fm3L6zZxcNLs3iwoe } 11, 53 -- X-Google-Smtp-Source: ADFU+vttqwpYeeGgojuWRhepav+vjsfSWPwdoBoZ4wOHhalNwUAOzE60ZtLJhw72LbYEdbjDf2RflQ== } 11, 53 -- X-Received: by 2002:a92:6b06:: with SMTP id g6mr12985242ilc.84.1584794761288; } 11, 53 -- Sat, 21 Mar 2020 05:46:01 -0700 (PDT) } 11, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:8069:12b3:af30:117f]) } 11, 53 -- by smtp.googlemail.com with ESMTPSA id 27sm3107763ilv.75.2020.03.21.05.46.00 } 11, 53 -- for {microscopy-at-microscopy.com} } 11, 53 -- (version=TLS1_3 cipher=TLS_AES_128_GCM_SHA256 bits=128/128); } 11, 53 -- Sat, 21 Mar 2020 05:46:01 -0700 (PDT) } 11, 53 -- Subject: viaWWW: How to clean nickel shim of magnetic and or glass particles? 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12, 29 -- To: "Microscopy-at-microscopy.com" {microscopy-at-microscopy.com} , nmz787-at-gmail.com 12, 29 -- References: {202003211355.02LDtIt2008929-at-microscopy.com} 12, 29 -- From: stefan diller {diller-at-stefan-diller.com} 12, 29 -- Message-ID: {4488c29b-7c90-12c6-6981-c8e1f6800948-at-stefan-diller.com} 12, 29 -- Date: Sat, 21 Mar 2020 17:59:08 +0100 12, 29 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:68.0) 12, 29 -- Gecko/20100101 Thunderbird/68.6.0 12, 29 -- MIME-Version: 1.0 12, 29 -- In-Reply-To: {202003211355.02LDtIt2008929-at-microscopy.com} 12, 29 -- Content-Type: text/plain; charset=windows-1252; format=flowed 12, 29 -- Content-Transfer-Encoding: 7bit 12, 29 -- Content-Language: en-GB 12, 29 -- X-Envelope-From: {diller-at-stefan-diller.com} 12, 29 -- X-Scanned-By: rockenstein AG ==============================End of - Headers==============================
if nobody can help you nearby and as long as Corona will stay outside my lab we can try set something as a life conference from my lab (I might need some help for this, so it might take some time to set it up...).
I am using a FE-SEM TESCAN MIRA3 and a Philips / FEI TEM EM420.
Some nice resources can be found by searching at google for "teaching electron microscopy"
and here
https://www.fei.com/education-resources/
For some outreach to your students you can use my nanoflight channel at vimeo to show what is possible with electron microscopy and a lot of effort.
https://vimeo.com/channels/nanoflight
and if you like I can put some more 3D nanoflights online for the OCULUS or other 3D glasses.
Best wishes,
Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
To clean a surface of particulates, I would use replicating tape. This is a cellulose acetate tape (non-adhesive) that you soften with acetone and press down onto the surface. Let it dry and peel it off. All the particulates should come with it. I've had better luck in removing particulates this way compared with ultrasonics, rinses, etc.
I'm not sure if an adhesive tape will work but if you don't have replicating tape, you might try some of the tape with the "Post-it" type adhesive. It may take several applications to remove everything. Replicating tape is available from most of the EM supply houses. It comes in both a thick and thin form. Cheers, Henk
----------------
Hendrik O. Colijn Center for Electron Microscopy and AnalysiS The Ohio State University 1305 Kinnear Rd, Suite 100
colijn.1-at-osu.edu 614/643-3458 cemas.osu.edu
-----Original Message----- X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Saturday, March 21, 2020 9:53 AM To: Colijn, Hendrik {colijn.1-at-osu.edu}
Hi Stephen,
Depending on the size of your class - you can use one of remote desktop packages, such as Teamviwer, VNC, RDP, Chrome remote desktop, etc... to let your students see (and even operate) GUI of the SEM, and hear your explanations.
I've been using TeamViewer for remote troubleshooting and training of FIB operators a couple of years, although haven't tried it in "classroom" kind of approach.
Best Wishes, Valery
Valery Ray (also with REFINE Lab, UCONN) ========================================== MEO Engineering Company, Inc. DBA "PBS&T" 290 Broadway, Suite 298, Methuen MA 01844 www.partbeamsystech.com www.freudlabs.com E-mail: vray-at-partbeamsystech.com Phone: +1-978-305-0479
On 3/21/2020 9:43 AM, microscopy.listserver-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: Stephen.Beck-at-ncc.edu } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy to both } Stephen.Beck-at-ncc.edu, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can } benefit from our collective wisdom } --------------------------------------------------------------------------- } } Email: Stephen.Beck-at-ncc.edu Name: Steve Beck } } Organization: Nassau Community College } } Title-Subject: [Filtered] Teaching SEM Online } } Message: Dear Colleagues, } I am teaching my SEM course this semester and like many institutions, we are trying to teach } online/remotely as our campus is closed due to COVID-19. I have administered my midterm exam online, } however, after that we need to get on the SEM to image the required samples. } Does anyone have any ideas regarding teaching SEM online? I have informed my administration that } this is impossible - I haven't received the courtesy of a response yet! } } Thanks! } Login Host: 173.77.159.14 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } ==============================Original Headers============================== } 8, 53 -- From microscopy.listserver-at-gmail.com Sat Mar 21 08:42:55 2020 } 8, 53 -- Received: from mail-io1-f52.google.com (mail-io1-f52.google.com [209.85.166.52]) } 8, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 02LDgtJo026786 } 8, 53 -- for {microscopy-at-microscopy.com} ; Sat, 21 Mar 2020 08:42:55 -0500 } 8, 53 -- Received: by mail-io1-f52.google.com with SMTP id h131so9143628iof.1 } 8, 53 -- for {microscopy-at-microscopy.com} ; Sat, 21 Mar 2020 05:41:56 -0700 (PDT) } 8, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 8, 53 -- d=gmail.com; s=20161025; } 8, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 8, 53 -- :in-reply-to:content-language:content-transfer-encoding; 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X-from: David Huskisson {david-at-emanalytical.co.uk}
Hi Stephen,
We have a couple of our SEMs connected to the internet for remote access. Through this interface, the microscope is completely controllable from your own PC. We can load your samples and then you or your students would be able to take control of the instrument and practice from your own homes.
This message is private and confidential. If received in error, please destroy and notify sender. Sender does not intend to waive confidentiality or privilege. Dissemination, use of or reliance upon this email is prohibited when received in error. Email may be susceptible to data corruption, interception and unauthorised amendment, and no liability is accepted by the sender for any of the foregoing. It is the recipient’s responsibility to scan the email and any attachment for viruses.
On Sat, Mar 21, 2020 at 1:48 PM {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote:
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Email: Stephen.Beck-at-ncc.edu {mailto:Stephen.Beck-at-ncc.edu} Name: Steve Beck
Organization: Nassau Community College
Title-Subject: [Filtered] Teaching SEM Online
Message: Dear Colleagues, I am teaching my SEM course this semester and like many institutions, we are trying to teach online/remotely as our campus is closed due to COVID-19. I have administered my midterm exam online, however, after that we need to get on the SEM to image the required samples. Does anyone have any ideas regarding teaching SEM online? I have informed my administration that this is impossible - I haven't received the courtesy of a response yet!
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From kelnanc57ctyee-at-gmail.com Sat Mar 28 20:49:33 2020 Return-Path: {kelnanc57ctyee-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [123.11.215.48] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id 02T1nWc6020483 for {microscopylistserverarchive7-at-microscopy.com} ; Sat, 28 Mar 2020 20:49:33 -0500 Message-ID: {4660B6DC.5BA45810-at-gmail.com}
X-from: Erico Freitas {ericotadeu-at-ufmg.br}
Dear all,
I'm just forwarding you the following TEM/AFM Researcher position (191486) at CNPEM.
The CNPEM is located in Campinas, S=C3=A3o Paulo state, Brazil.
This position is likely for the LNNano (Brazilian National Nanotechnology Laboratory) at CNPEM (https://lnnano.cnpem.br/)
Cheers,
-- Erico Freitas
Physicist/Microscopist at Center of Microscopy Universidade Federal de Minas Gerais (UFMG) Av. Antônio Carlos, 6627, Campus Pampulha, Belo Horizonte, MG, Brazil. ZIP Code 31270-901. +55-31-3409-7573 +55-31-3409-7575
Coordinator:Transmission Electron Microscopy laboratory
This position is likely for the LNNano (Brazilian National Nanotechnology Laboratory) at CNPEM (https://lnnano.cnpem.br/)
Cheers,
-- Erico Freitas
Physicist/Microscopist at Center of Microscopy Universidade Federal de Minas Gerais (UFMG) Av. Antônio Carlos, 6627, Campus Pampulha, Belo Horizonte, MG, Brazil. ZIP Code 31270-901. +55-31-3409-7573 +55-31-3409-7575
Coordinator:Transmission Electron Microscopy laboratory
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Email: phillipa.timmins-at-aurox.co.uk
Name: Phillipa Timmins
Organization: Aurox Ltd
Title-Subject: [Filtered] Aurox On-line Conference on Microscopy 7th and 8th April 2020
Message: Dear All,
I hope you are all staying safe in these troubling and turbulent times. We send out our best wishes from Aurox.
We have had a hugely positive response to our upcoming On-line Conference on Microscopy on 7th and 8th April. We have a fantastic line up of speakers from academia and industry covering a range of topics including: Raman Microscopy, Optical Diffraction Tomography, Cryo-microscopy, Super resolution, Live cell imaging, AI, Image processing, Adaptive Optics, Mesolens, Expansion Microscopy as well as keynote by Tony Wilson and updates from various companies.
I just wanted to let you know that the deadline to register your interest in attending is this Friday at 2PM UK time.
It is free to attend and you can find the program and sign up page here:
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Email: xbren-at-uw.edu Name: Shirley
Organization: UWMC
Title-Subject: [Filtered] Clinical EM lab
Message: Hello colleagues, Does anyone know how many clinical EM labs are in America? our department manager would like to know, and may need help in the future.
Thank you,
Shirley
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From andralbi54nuxdo-at-gmail.com Sat Apr 4 22:18:40 2020 Return-Path: {andralbi54nuxdo-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [123.11.215.6] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id 0353IdLn030280 for {microscopylistserverarchive7-at-microscopy.com} ; Sat, 4 Apr 2020 22:18:40 -0500 Message-ID: {413101d60ab5$fbf3b720$ce8d52e7-at-andralbi54nuxdo}
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Email: xbren-at-uw.edu Name: Shirley
Organization: UWMC
Title-Subject: [Filtered] Clinical EM lab
Message: Hello colleagues, Does anyone know how many clinical EM labs are in America? our department manager would like to know, and may need help in the future.
Thank you,
Shirley
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both leroy-at-icmpe.cnrs.fr, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Email: leroy-at-icmpe.cnrs.fr
Name: Eric Leroy
Organization: CNRS
Title-Subject: [Filtered] Vintage DTSA
Message: Hi, I am searching for the old DTSA software. The web page of the NIST still exists but all the download links are dead. Does anybody have a backup of DTSA 2.5 or 3.1 ? Thank you by advance, Best regards, Eric
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From solizbula031axig-at-gmail.com Wed Apr 8 17:16:46 2020 Return-Path: {solizbula031axig-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [123.11.215.50] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id 038MGh6J023039 for {microscopylistserverarchive7-at-microscopy.com} ; Wed, 8 Apr 2020 17:16:45 -0500 Message-ID: {A16504D1.F1B47E53-at-gmail.com}
Hello Eric,
if you still need it: I found sea.hqx archive files of PowerDTSAv2.5.1 and PPC_DTSA301 on my computer.
Also the manual.
Best wishes,
Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
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Email: srousselle-at-stagebio.com
Name: Serge Rousselle
Organization: StageBio
Title-Subject: [Filtered] SEM sample prep: Critical Point Drying and Sputter Coating
Message: 1) Looking for drying chamber and sputter coating equipment for SEM sample preparation that can accommodate large specimens (up to 5cm in diameter or up to 8-10cm long with a narrower diameter such as a large vessel). What equipment would you recommen?
2) Anyone able to provide remote/web-based training for SEM sample preparation (not imaging, just sample prep)? Thank you Serge
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I am a materials scientist and not a biologist, but I will offer some ideas.
I have only seen a couple CPD systems. I don't think they would have been able to accommodate such a large sample. There may be some that could, but I think they would be rare. I recall you will also have issues with thickness. The processing time will depend on the thickness and if all the alcohol is not removed, you will disrupt your structure.
We purchased a Denton sputter coater with a turntable designed to evenly coat samples up to 6 inches in diameter. It worked, but was slower than the standard coater. There was a sectored aperture in front of the target to ensure even coating and that slowed the rate per unit area, and then there was much more area to cover so more time was required.
There may also be issues with the SEM. Our FEI Quanta is the 250 model with a 2-inch stage. Our low-mag limit is 50x at 10 mm working distance, so we can only image 2mm x 1.5mm at a time. We can only survey a 2-inch x 2-inch area at a time. We can load larger samples, but we would have to remove and reset them in order to cover the entire area.
I think you will also find that you may not have good coating over the entire surface. It depends on how convoluted your sample is. You may need to ground the surface in many places to make sure the charge can bleed away to the stage.
With all those issues, I wonder if you might be better off to prepare multiple, smaller samples that can be dehydrated, coated, and imaged using readily available equipment. Hopefully the features of interest would not fall only on the cut lines between the sub-samples.
Also, do you need high magnification to look at small surface structures? If not, the variable pressure or environmental modes of an SEM like the Quanta might not require any sample preparation. It depends on what you want to see.
Warren Straszheim Materials Analysis and Research Lab Iowa State University
-----Original Message----- X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Thursday, April 09, 2020 6:35 AM To: Straszheim, Warren E [BIOTC] {wesaia-at-iastate.edu}
X-from: srousselle-at-stagebio.com
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Email: srousselle-at-stagebio.com
Name: Serge Rousselle
Organization: StageBio
Title-Subject: [Filtered] SEM sample prep: Critical Point Drying and Sputter Coating
Message: 1) Looking for drying chamber and sputter coating equipment for SEM sample preparation that can accommodate large specimens (up to 5cm in diameter or up to 8-10cm long with a narrower diameter such as a large vessel). What equipment would you recommen?
2) Anyone able to provide remote/web-based training for SEM sample preparation (not imaging, just sample prep)? Thank you Serge
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From smithejoseph18faoog-at-gmail.com Thu Apr 9 17:47:00 2020 Return-Path: {smithejoseph18faoog-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [123.11.215.30] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id 039Mk2YZ002860 for {microscopylistserverarchive7-at-microscopy.com} ; Thu, 9 Apr 2020 17:46:57 -0500 Received: from unknown (131.27.23.190) by mx03.listsystemsf.net with NNFMP; Thu, 09 Apr 2020 17:34:24 -0500 Received: from smtp-server1.cfdenselr.com ([Thu, 09 Apr 2020 17:30:53 -0500]) by mail.naihautsui.co.kr with SMTP; Thu, 09 Apr 2020 17:30:53 -0500 Received: from [76.3.117.196] by mmx09.tilkbans.com with QMQP; Thu, 09 Apr 2020 17:16:38 -0500 Received: from external.newsubdomain.com ([116.202.95.28]) by mts.locks.grgtween.net with QMQP; Thu, 09 Apr 2020 17:15:01 -0500 Message-ID: {564FB272.034354B5-at-gmail.com}
X-from: Desert Rat {desertrat99-at-verizon.net}
Hi folks,
I have a friend who is trying to identify the features in some SEM images but they are bio and I am hard materials. The images are at the following link https://drive.google.com/drive/folders/1vzKbmfoDZT6f9fsJduTgxx_hwKj1-sGE?usp=sharing They were collected from a white microbial mat in a cave at Lava Beds National Monument with NPS permission. They were mounted to SEM stubs with carbon tape and dried in a vacuum oven. Then coated in gold/palladium and put in the SEM. They think the big, 100 micron, thing is a mite? Any other idea welcome. What they really need to know is what are the little wormy things that look like chains of balls |--500nm in diameter? They think they might be bacteria eating the dead mite?
Nick
--
Dr Nicholas Seaton
SEM, FIB & LM Specialist Department Safety Officer Characterization Facility
College of Science and Engineering
University of Minnesota
12 Shepherd Labs
100 Union Street SE
Minneapolis
MN, 55455
email: seato008-at-umn.edu
phone: 612-626-5314
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I am also a materials guy rather than a biologist.
I am inclined to agree with your identification of a mite of some kind.
I don't think the semicircular features are bacteria. The size may be about right, but I don't think the distribution is. I wonder if it might be some sort of exude from the drying process.
I think the very fine structures in the 3500x and 12,000x images are coating grains. I have seen similar things before where the coating does not "like" the substrate. It "beads up", if you will and creates those recognizable structures. I wonder what an uncoated version of the sample would look like.
Warren Straszheim Materials Analysis and Research Lab Iowa State University
-----Original Message----- X-from: seato008-at-umn.edu [mailto:seato008-at-umn.edu] Sent: Saturday, April 11, 2020 11:56 AM To: Straszheim, Warren E [BIOTC] {wesaia-at-iastate.edu}
Hi folks,
I have a friend who is trying to identify the features in some SEM images but they are bio and I am hard materials. The images are at the following link https://drive.google.com/drive/folders/1vzKbmfoDZT6f9fsJduTgxx_hwKj1-sGE?usp=sharing They were collected from a white microbial mat in a cave at Lava Beds National Monument with NPS permission. They were mounted to SEM stubs with carbon tape and dried in a vacuum oven. Then coated in gold/palladium and put in the SEM. They think the big, 100 micron, thing is a mite? Any other idea welcome. What they really need to know is what are the little wormy things that look like chains of balls |--500nm in diameter? They think they might be bacteria eating the dead mite?
Nick
--
Dr Nicholas Seaton
SEM, FIB & LM Specialist Department Safety Officer Characterization Facility
College of Science and Engineering
University of Minnesota
12 Shepherd Labs
100 Union Street SE
Minneapolis
MN, 55455
email: seato008-at-umn.edu
phone: 612-626-5314
==============================Original Headers============================== 16, 54 -- From seato008-at-umn.edu Sat Apr 11 11:54:59 2020 16, 54 -- Received: from mta-p8.oit.umn.edu (mta-p8.oit.umn.edu [134.84.196.208]) 16, 54 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 03BGswrx002108 16, 54 -- for {Microscopy-at-microscopy.com} ; Sat, 11 Apr 2020 11:54:59 -0500 16, 54 -- Received: from localhost (unknown [127.0.0.1]) 16, 54 -- by mta-p8.oit.umn.edu (Postfix) with ESMTP id 4901HH4WXDz9vKYm 16, 54 -- for {Microscopy-at-microscopy.com} ; Sat, 11 Apr 2020 16:56:11 +0000 (UTC) 16, 54 -- X-Virus-Scanned: amavisd-new at umn.edu 16, 54 -- Received: from mta-p8.oit.umn.edu ([127.0.0.1]) 16, 54 -- by localhost (mta-p8.oit.umn.edu [127.0.0.1]) (amavisd-new, port 10024) 16, 54 -- with ESMTP id aXkuqK2uE4lh for {Microscopy-at-microscopy.com} ; 16, 54 -- Sat, 11 Apr 2020 11:56:11 -0500 (CDT) 16, 54 -- Received: from mail-io1-f69.google.com (mail-io1-f69.google.com [209.85.166.69]) 16, 54 -- (using TLSv1.2 with cipher ECDHE-RSA-AES128-GCM-SHA256 (128/128 bits)) 16, 54 -- (No client certificate requested) 16, 54 -- by mta-p8.oit.umn.edu (Postfix) with ESMTPS id 4901HH3R9sz9vKYh 16, 54 -- for {Microscopy-at-microscopy.com} ; Sat, 11 Apr 2020 11:56:11 -0500 (CDT) 16, 54 -- Received: by mail-io1-f69.google.com with SMTP id a12so5310757ioe.17 16, 54 -- for {Microscopy-at-microscopy.com} ; Sat, 11 Apr 2020 09:56:11 -0700 (PDT) 16, 54 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 16, 54 -- d=umn.edu; s=google; 16, 54 -- h=mime-version:from:date:message-id:subject:to; 16, 54 -- bh=pNRK4qwo1Kl/3La6hMDxJ7xSbHi5dvlta3VlKU/V+MA=; 16, 54 -- b=p9gHfS1zhZDhn59d83ZJsC9WaEdGXmFPAYTReNCbo9+WW2B2+34E9y+XDJnYTO5CVF 16, 54 -- ieE6nqqD3nERNCVoe5lckTPOiLeGNTK3xORfYMQqwKSvKxh1jpYsydIC1qn0S26daZ1e 16, 54 -- F7G3tnDwIwM6Xt8eJw5wkk6rSYclznMn1IYfp0py3ZIjGAsyjwY9uDJCEkDZ1Sz7XmiP 16, 54 -- KqlKQb6Fxg7FJRSsPyOG+pSqk4BCKtQfOoYV7Yi5pE9hhbzkoGekI5ewhv3ShLcDho2b 16, 54 -- OPDodGIqQ6GVwHw8aNMCZltTr3JADbEISP2eNstVM69Yw+zZlUfGxyQXWwchrZTdY9r4 16, 54 -- cTmg== 16, 54 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 16, 54 -- d=1e100.net; s=20161025; 16, 54 -- h=x-gm-message-state:mime-version:from:date:message-id:subject:to; 16, 54 -- bh=pNRK4qwo1Kl/3La6hMDxJ7xSbHi5dvlta3VlKU/V+MA=; 16, 54 -- b=arGaRjpcZdvMQdGrgMLOiCnbEqhvGNxFbsHM+NePALW1jnGZktAiZYEQ5wjVrWo2uL 16, 54 -- 2cjalp/dY4qRGSNGRGfH4yrpiCTZZcXSxY4sHTuQbV6Eqvx0rDFUzYXu4FM+KVjLJ/pa 16, 54 -- TD7Jx5aqHZuaNNFqNg2KSuyl9uVd84xyI1FallIhccX5DI+Wzho3Y3Dl6fO84X0H9+5B 16, 54 -- oW0SKlb6C1wyPDuFhKvNBNbaKxvd1lT5CuM6KHXrfK+7rrTzkiUuOs9sk6vSfUluo8B5 16, 54 -- KdGt+mCNYF2ZDuEY9+pcxoW38gRZfY84QUuN9Uv/DOA/b7aKWThXiFEzv1f5LxZXza1h 16, 54 -- gMgg== 16, 54 -- X-Gm-Message-State: AGi0PuapHwgIK3MZU0O1DKCkqngfvA5AsGw7OzK553UJODZEbptUWBnu 16, 54 -- q+/mQ2rxQOPrQCrI3IrfUz5nmO0zMwYGrJKwp7IBpJMdsMFQptWqRim8Ph+aWhYvowbFxXx43z1 16, 54 -- q7qTEjMAchZo+FgsvzwKispzSBqnzqwvFVdaq/xAA 16, 54 -- X-Received: by 2002:a05:6602:1da:: with SMTP id w26mr9302826iot.191.1586624170865; 16, 54 -- Sat, 11 Apr 2020 09:56:10 -0700 (PDT) 16, 54 -- X-Google-Smtp-Source: APiQypI5UqP3RYAu1aj6ARd/tbQMtSFoGB5rP1sVnhaMt3kD78qSa7b60yiCUQGh8HZ0wqN3LaFtLVZ7/HJnC86z9fU= 16, 54 -- X-Received: by 2002:a05:6602:1da:: with SMTP id w26mr9302817iot.191.1586624170591; 16, 54 -- Sat, 11 Apr 2020 09:56:10 -0700 (PDT) 16, 54 -- MIME-Version: 1.0 16, 54 -- From: Nick Seaton {seato008-at-umn.edu} 16, 54 -- Date: Sat, 11 Apr 2020 11:55:59 -0500 16, 54 -- Message-ID: {CAMJSFzKnWoBhQP5oft0XZppEOaqR2Jq48SFmSQMZwR2TdSMQKg-at-mail.gmail.com} 16, 54 -- Subject: SEM images of microbes 16, 54 -- To: Microscopy-at-microscopy.com 16, 54 -- Content-Type: text/plain; charset="UTF-8" ==============================End of - Headers==============================
Tel/fax +30 210 8957677 mobile +30 6945 107477 www.eikonika.netwww.aim.cat *************************************
-----Original Message----- X-from: wesaia-at-iastate.edu [mailto:wesaia-at-iastate.edu] Sent: Saturday, April 11, 2020 20:43 To: eikonika-at-otenet.gr
I am also a materials guy rather than a biologist.
I am inclined to agree with your identification of a mite of some kind.
I don't think the semicircular features are bacteria. The size may be about right, but I don't think the distribution is. I wonder if it might be some sort of exude from the drying process.
I think the very fine structures in the 3500x and 12,000x images are coating grains. I have seen similar things before where the coating does not "like" the substrate. It "beads up", if you will and creates those recognizable structures. I wonder what an uncoated version of the sample would look like.
Warren Straszheim Materials Analysis and Research Lab Iowa State University
-----Original Message----- X-from: seato008-at-umn.edu [mailto:seato008-at-umn.edu] Sent: Saturday, April 11, 2020 11:56 AM To: Straszheim, Warren E [BIOTC] {wesaia-at-iastate.edu}
Hi folks,
I have a friend who is trying to identify the features in some SEM images but they are bio and I am hard materials. The images are at the following link https://drive.google.com/drive/folders/1vzKbmfoDZT6f9fsJduTgxx_hwKj1-sGE?usp =sharing They were collected from a white microbial mat in a cave at Lava Beds National Monument with NPS permission. They were mounted to SEM stubs with carbon tape and dried in a vacuum oven. Then coated in gold/palladium and put in the SEM. They think the big, 100 micron, thing is a mite? Any other idea welcome. What they really need to know is what are the little wormy things that look like chains of balls |--500nm in diameter? They think they might be bacteria eating the dead mite?
Nick
--
Dr Nicholas Seaton
SEM, FIB & LM Specialist Department Safety Officer Characterization Facility
College of Science and Engineering
University of Minnesota
12 Shepherd Labs
100 Union Street SE
Minneapolis
MN, 55455
email: seato008-at-umn.edu
phone: 612-626-5314
==============================Original Headers============================== 16, 54 -- From seato008-at-umn.edu Sat Apr 11 11:54:59 2020 16, 54 -- Received: from mta-p8.oit.umn.edu (mta-p8.oit.umn.edu [134.84.196.208]) 16, 54 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 03BGswrx002108 16, 54 -- for {Microscopy-at-microscopy.com} ; Sat, 11 Apr 2020 11:54:59 -0500 16, 54 -- Received: from localhost (unknown [127.0.0.1]) 16, 54 -- by mta-p8.oit.umn.edu (Postfix) with ESMTP id 4901HH4WXDz9vKYm 16, 54 -- for {Microscopy-at-microscopy.com} ; Sat, 11 Apr 2020 16:56:11 +0000 (UTC) 16, 54 -- X-Virus-Scanned: amavisd-new at umn.edu 16, 54 -- Received: from mta-p8.oit.umn.edu ([127.0.0.1]) 16, 54 -- by localhost (mta-p8.oit.umn.edu [127.0.0.1]) (amavisd-new, port 10024) 16, 54 -- with ESMTP id aXkuqK2uE4lh for {Microscopy-at-microscopy.com} ; 16, 54 -- Sat, 11 Apr 2020 11:56:11 -0500 (CDT) 16, 54 -- Received: from mail-io1-f69.google.com (mail-io1-f69.google.com [209.85.166.69]) 16, 54 -- (using TLSv1.2 with cipher ECDHE-RSA-AES128-GCM-SHA256 (128/128 bits)) 16, 54 -- (No client certificate requested) 16, 54 -- by mta-p8.oit.umn.edu (Postfix) with ESMTPS id 4901HH3R9sz9vKYh 16, 54 -- for {Microscopy-at-microscopy.com} ; Sat, 11 Apr 2020 11:56:11 -0500 (CDT) 16, 54 -- Received: by mail-io1-f69.google.com with SMTP id a12so5310757ioe.17 16, 54 -- for {Microscopy-at-microscopy.com} ; Sat, 11 Apr 2020 09:56:11 -0700 (PDT) 16, 54 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 16, 54 -- d=umn.edu; s=google; 16, 54 -- h=mime-version:from:date:message-id:subject:to; 16, 54 -- bh=pNRK4qwo1Kl/3La6hMDxJ7xSbHi5dvlta3VlKU/V+MA=; 16, 54 -- b=p9gHfS1zhZDhn59d83ZJsC9WaEdGXmFPAYTReNCbo9+WW2B2+34E9y+XDJnYTO5CVF 16, 54 -- ieE6nqqD3nERNCVoe5lckTPOiLeGNTK3xORfYMQqwKSvKxh1jpYsydIC1qn0S26daZ1e 16, 54 -- F7G3tnDwIwM6Xt8eJw5wkk6rSYclznMn1IYfp0py3ZIjGAsyjwY9uDJCEkDZ1Sz7XmiP 16, 54 -- KqlKQb6Fxg7FJRSsPyOG+pSqk4BCKtQfOoYV7Yi5pE9hhbzkoGekI5ewhv3ShLcDho2b 16, 54 -- OPDodGIqQ6GVwHw8aNMCZltTr3JADbEISP2eNstVM69Yw+zZlUfGxyQXWwchrZTdY9r4 16, 54 -- cTmg== 16, 54 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 16, 54 -- d=1e100.net; s=20161025; 16, 54 -- h=x-gm-message-state:mime-version:from:date:message-id:subject:to; 16, 54 -- bh=pNRK4qwo1Kl/3La6hMDxJ7xSbHi5dvlta3VlKU/V+MA=; 16, 54 -- b=arGaRjpcZdvMQdGrgMLOiCnbEqhvGNxFbsHM+NePALW1jnGZktAiZYEQ5wjVrWo2uL 16, 54 -- 2cjalp/dY4qRGSNGRGfH4yrpiCTZZcXSxY4sHTuQbV6Eqvx0rDFUzYXu4FM+KVjLJ/pa 16, 54 -- TD7Jx5aqHZuaNNFqNg2KSuyl9uVd84xyI1FallIhccX5DI+Wzho3Y3Dl6fO84X0H9+5B 16, 54 -- oW0SKlb6C1wyPDuFhKvNBNbaKxvd1lT5CuM6KHXrfK+7rrTzkiUuOs9sk6vSfUluo8B5 16, 54 -- KdGt+mCNYF2ZDuEY9+pcxoW38gRZfY84QUuN9Uv/DOA/b7aKWThXiFEzv1f5LxZXza1h 16, 54 -- gMgg== 16, 54 -- X-Gm-Message-State: AGi0PuapHwgIK3MZU0O1DKCkqngfvA5AsGw7OzK553UJODZEbptUWBnu 16, 54 -- q+/mQ2rxQOPrQCrI3IrfUz5nmO0zMwYGrJKwp7IBpJMdsMFQptWqRim8Ph+aWhYvowbFxXx43z1 16, 54 -- q7qTEjMAchZo+FgsvzwKispzSBqnzqwvFVdaq/xAA 16, 54 -- X-Received: by 2002:a05:6602:1da:: with SMTP id w26mr9302826iot.191.1586624170865; 16, 54 -- Sat, 11 Apr 2020 09:56:10 -0700 (PDT) 16, 54 -- X-Google-Smtp-Source: APiQypI5UqP3RYAu1aj6ARd/tbQMtSFoGB5rP1sVnhaMt3kD78qSa7b60yiCUQGh8HZ0wqN3LaFt LVZ7/HJnC86z9fU= 16, 54 -- X-Received: by 2002:a05:6602:1da:: with SMTP id w26mr9302817iot.191.1586624170591; 16, 54 -- Sat, 11 Apr 2020 09:56:10 -0700 (PDT) 16, 54 -- MIME-Version: 1.0 16, 54 -- From: Nick Seaton {seato008-at-umn.edu} 16, 54 -- Date: Sat, 11 Apr 2020 11:55:59 -0500 16, 54 -- Message-ID: {CAMJSFzKnWoBhQP5oft0XZppEOaqR2Jq48SFmSQMZwR2TdSMQKg-at-mail.gmail.com} 16, 54 -- Subject: SEM images of microbes 16, 54 -- To: Microscopy-at-microscopy.com 16, 54 -- Content-Type: text/plain; charset="UTF-8" ==============================End of - Headers==============================
The big thing looks like an Oribatid mite. The setation (pattern and morphology of the hairs) would probably ID at least the family, but I don't have the necessary keys. The other 2 images ... hm. The "wormy things" could be bacterial. They might be part of the mite. Some mites do have some pretty intricate decorations. I don't think the "worms", are a coating artifact - I've never seen an artifact like this, certainly. The smaller rosettes might be coating artifact, but I don't think they are either, the shapes are too intricate and consistent.
What were the coating parameters? Time, pressure, mA?
Phil ----------------------------------------- Philip Oshel Imaging Center Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576 office (989) 774-7567 lab
________________________________________ X-from: seato008-at-umn.edu {seato008-at-umn.edu} Sent: Saturday, April 11, 2020 13:01 To: Oshel, Philip Eugene
Hi folks,
I have a friend who is trying to identify the features in some SEM images but they are bio and I am hard materials. The images are at the following link https://drive.google.com/drive/folders/1vzKbmfoDZT6f9fsJduTgxx_hwKj1-sGE?usp=sharing They were collected from a white microbial mat in a cave at Lava Beds National Monument with NPS permission. They were mounted to SEM stubs with carbon tape and dried in a vacuum oven. Then coated in gold/palladium and put in the SEM. They think the big, 100 micron, thing is a mite? Any other idea welcome. What they really need to know is what are the little wormy things that look like chains of balls |--500nm in diameter? They think they might be bacteria eating the dead mite?
Nick
--
Dr Nicholas Seaton
SEM, FIB & LM Specialist Department Safety Officer Characterization Facility
College of Science and Engineering
University of Minnesota
12 Shepherd Labs
100 Union Street SE
Minneapolis
MN, 55455
email: seato008-at-umn.edu
phone: 612-626-5314
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Email: joern1911-at-gmail.com
Name: Joern
Organization: service Sem
Title-Subject: [Filtered] Leo S430 EO Board
Message: Hello Anybody,
please can you Help Please?
I have a Leo S430 SEM.
On my EO Board is a Error on C1 Coil . i have 3 ampere on the Output.
On my EO-Board is the Vision with 2 OP . I have found a Schcematic with 1 Op for the C1 Output. I think , the first Op is for the Setpoint and the second Op is for actual value from the Output currend from the C1 Coil Lens.
Have a anybody a schcematic from the EO_Board.
Thanks and best regards
Joern
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We know some institutions are shutting down completely during this pandemic. Our facility is still open (we have been designated as essential and will be required to stay open if at all possible). If you can't access your usual EM facilities, we would be glad to help. We will provide you with a D.O.T. approved shipping container kit for you to use to send your samples to us. If you need help contact me.
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Email: pmccurdy-at-colostate.edu Name: Pat McCurdy
Organization: Colorado State University
Title-Subject: [Filtered] Best carbon coating method
Message: To Whom It May Concern:
We are seeking to purchase a new carbon coater. Our center does EDS and EBSD. We would like to coat down to 5 nm of carbon. We are looking at both carbon rod coaters as well as a carbon thread coater. I would appreciate your input on these two types of coaters or any additional kinds that you may have any experience with.
Thanks, Pat McCurdy Colorado State University
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With due disclaimer that (a) I've been doing C coatings mostly for charge mitigation on FIB Circuit Edit samples, and (b) below are personal impressions and not a conclusion from any kind of comparative study:
The best (perceived as smoothest, cleanest, and most uniform) carbon coatings I've seen were produced by Gatan's PECS system, using ion sputtering. I haven't operated PECS myself, but coatings made in it for me were just perfect.
Overall impression is that good cord and rod evaporation coatings typically come from turbo-pumped systems run by an operator with enough patience to wait for a full pump-down. I have been using high-vacuum version of Safematic, and despite of my initial skepticism am very pleased with it. Automated exchange of evaporation cord is oh so convenient..
No vested interest in Gatan/AMETEK or Safematic here....
Valery
Valery Ray (also with REFINE Lab, UCONN) ========================================== MEO Engineering Company, Inc. DBA "PBS&T" 290 Broadway, Suite 298, Methuen MA 01844 www.partbeamsystech.com www.freudlabs.com E-mail: vray-at-partbeamsystech.com Phone: +1-978-305-0479
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