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Email: jpare-at-emory.edu Name: Jeff Pare
Organization: Emory University\Yerkes National Primate Center
Title-Subject: [Filtered] SBF/SEM
Message: Good morning everybody. Happy New Year to all! I am a lab supervisor at Yerkes and we are using a company to perform "serial face block scanning electron microscopy" of brain tissue. We are interested in finding other companies who perform that type of work. Do you know of groups or companies that do SBF/SEM? If so please contact me at jpare-at-emory.edu
Thanks
Jeff
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Message: If you still have the 1988 Proceedings, may I ask you to do me a favour, please, and scan the following abstract by Dr. Gordon W. Ellis who is no longer alive:
We have multiple open positions in the NUANCE Center here at Northwestern University. Please see brief position descriptions and application info below. Feel free to reach out if you want more information and please forward to anyone who may be interested.
Regards, Ben
1. SEM Facility Manager The SEM Facility Manager is responsible for all aspects of the SEM/FIB Facility. This includes: equipment purchasing, upgrades and maintenance; supervision of Microscopy and Imaging Specialist; user training and technical support on all SEM instruments and related equipment; course development and teaching of laboratory components of SEM related courses; development of new analytical techniques and capabilities; providing analytical services for both internal NU and external industrial clients; conducting facility tours and demonstrations. In addition, this position has primary technical responsibility for the dual-beam Focused Ion Beam (FIB) instrument and assists in sample preparation for all electron microscopy, including Transmission Electron Microscopy (TEM).
MS/PhD, 4-5+ years hands-on characterization experience (post-degree) and 2+ years facility management/user training experience preferred.
2. TEM Facility Manager The TEM Facility Manager is responsible for training and assisting users on conventional and advanced Scanning-Transmission and Transmission Electron Microscopy (S/TEM), facilitating laboratory sessions for microscopy courses, and conducting analysis and characterization using advanced electron microscopes.
The successful candidate will develop an informed and well-trained user base in advanced microscopy and related sample preparation and characterization instruments. The incumbent will participate in user training, hands-on assistance, development of professional and short-courses, and overall facility development. Essential to the success of this position is enhancing both the visibility and advanced utilization of the S/TEM instruments to promote scholarly activities among the faculty, student and staff user base.
The selected candidate will regularly operate conventional and advanced S/TEM analysis, consult and collaborate with users on experiment design & research in electron microscopy, sample preparation and general lab equipment. There are ample opportunities for independent and collaborative research utilizing advanced S/TEM. With the recent installation of an advanced aberration-corrected S/TEM instrument, prior experience with AC-S/TEM is desirable, but not required.
Materials should be submitted as a single PDF to mailto:nuance-at-northwestern.edu. Complete applications will include: Introduction letter, Curriculum Vitae, Statement of plans for research (3 to 5 pages), Contact information for three references (names, postal & email addresses, phone number).
3. TEM Postdoctoral Research Associate Under supervision of Dr. Xiaobing Hu, the selected candidate will develop advanced TEM applications and in-situ TEM methods on various materials system including engineering alloys, battery materials, thermoelectric materials and organic perovskite solar cells. The incumbent will oversee training of NUANCE users and collaborate with many excellent research groups in the department of Material Science and Engineering and Chemistry. As there will be ample opportunity to publish in top journals and assist in writing research and equipment proposals, the candidate must have excellent written and oral communication skills.
Research - Work closely with TEM manager to design and implement research methods in support of advanced materials research projects. Training - Design and implement training curriculum for new, intermediate and advanced TEM users (students, postdocs, research staff). Collaboration - Work with other faculty groups to design and implement TEM experiements specific to their research needs.
Applicants should send as a single PDF, 1. introduction letter (including research goals), 2. CV, 3. three references to Dr. Xiaobing Hu (mailto:xbhu-at-northwestern.edu).
----------------------------------------------------------------------------------------------------------- Ben Myers, PhD
Director of Operations SHyNE Resource, NUANCE Center
Northwestern University 2220 Campus Dr. Evanston, IL 60208 (847) 467-1081 shyne.northwestern.edu
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Email: unocicrr-at-ornl.gov Name: Ray Unocic
Organization: Oak Ridge National Laboratory
Title-Subject: [Filtered] Postdoctoral Position at ORNL on in situ microscopy/atomic manipulation
We are seeking a Postdoctoral Research Associate to support the development and application of directed atomic manipulation of materials using aberration corrected scanning transmission electron microscopy (STEM). The specific focus of your research will be to understand and control electron beam interactions to understand factors that will guide the directed fabrication and atomic manipulation of 1D, 2D, and 3D materials. You will contribute to the development of novel electron beam scan control and feedback strategies, perform in situ microscopy experiments under controlled environmental conditions and as a function of external stimuli, and you will work closely with Oak Ridge National Laboratory (ORNL) scientists on materials characterization, synthesis, and theory to experimentally validate predictive models. You will also collaborate with machine learning experts at ORNL to provide experimental data input.
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Email: mcconkie.thomas-at-gmail.com Name: Thomas McConkie
Organization: The Design Knowledge Company
Title-Subject: [Filtered] SEM simulation programs.
Message: I am looking for either opensource or for purchase SEM simulation programs. I am not looking for a virtual SEM for training but one that I can use to create theoretical images from known substrate patterns of varying materials like integrated circuits. I am new to the simulation SEM field and any information would be helpful.
Thank you.
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Email: roger.ristau-at-uconn.edu Name: Roger Ristau
Organization: University of Connecticut
Title-Subject: [Filtered] TEM apertures
Message: While describing the operation of the TEM to students, I note that all the conventional textbooks depict the objective aperture as below the objective lens, that is, they show the sequence in ray diagrams as: specimen, lower objective lens, objective aperture coincident with the back focal plane. The problem is that the objective aperture is phycically located above the lower objective lens in our TEM: i.e. specimen, objective aperture, lower objective lens. The thing I can't get my head around is how the back focal plane can be coincident with the objective lens in the second case? Or is this a case of a virtual aperture?
Confused in Connecticut
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Email: info-at-bpi.de Name: Susanne Wolf
Organization: Bavarian Polymer Institute
Title-Subject: [Filtered] Job Offer, University of Bayreuth, Bavarian Polymer Institute
Message: Dear All,
In the KeyLab "Electron and Optical Microscopy" of the Bavarian Polymer Institute of the University of Bayreuth, the following full-time position is to be filled as soon as possible, initially for a limited period:
Research Associate For the future Director of Electron Microscopy pay grade: 13 TV-L (100%)
Application Deadline: 15. February 2019
If you are interested, you can find more information at:
The OL is essentially an immersion lens; focusing occurs over an extended region between the top and bottom pole pieces that contains the specimen. The top pole piece contributes to forming parallel illumination on the specimen, and a crossover occurs above the lower pole piece, so an objective aperture can be situated below the specimen plane, but above the lower pole piece. A second crossover occurs below the OL, so our JEOL has apertures both within the lens and below the lens.
I wish the apertures were closer to the true back focal plane(s), though. They tend to limit the field of view at low magnifications.
-Phil -----Original Message----- X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Tuesday, January 8, 2019 4:29 PM To: Ahrenkiel, Phil {Phil.Ahrenkiel-at-sdsmt.edu}
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Email: roger.ristau-at-uconn.edu Name: Roger Ristau
Organization: University of Connecticut
Title-Subject: [Filtered] TEM apertures
Message: While describing the operation of the TEM to students, I note that all the conventional textbooks depict the objective aperture as below the objective lens, that is, they show the sequence in ray diagrams as: specimen, lower objective lens, objective aperture coincident with the back focal plane. The problem is that the objective aperture is phycically located above the lower objective lens in our TEM: i.e. specimen, objective aperture, lower objective lens. The thing I can't get my head around is how the back focal plane can be coincident with the objective lens in the second case? Or is this a case of a virtual aperture?
Confused in Connecticut
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Email: Ursel.Bangert-at-ul.ie Name: Ursel Bangert
Organization: Bernal Institute, University of Limerick
Title-Subject: [Filtered] Instrument Scientist (Electron Microscopy) position
Message: We have a job vacancy for an Instrument Scientist in Electron Microscopy at UL (see below), and we would appreciate very much if you could bring this to the attention of people (in your institution or elsewhere) who might be interested, and generally spread the word.
Title: Instrument Scientist (Electron Microscopy)
Job Description: The Bernal Institute at UL requires an Instrument Scientist to support the research activities of the Institute with focus on the area of transmission electron microscopy. The Institute has a double aberration corrected, monochromated FEI Titan Cubed Themis TEM with analytical facilities (SuperEDX and QuantumEELS) and sample holders for in-situ TEM measurements under heating, biasing, in liquids and gases as well as under cryo-conditions. This Titan has furthermore an ultra-fast and-sensitive (K2-IS) camera, for ultra-high resolution as well as in-situ imaging and spectroscopic analysis of materials. Requirements: PhD in materials science/electron microscopy/chemistry/physics/ engineering or a related discipline OR equivalent experience in a research environment (equivalent 4 years fulltime research after primary degree). The full description and information for the position can be found at the following link : http://www.ul.ie/hrvacancies/ . For additional information contact Prof Ursel Bangert (ursel.bangert-at-ul.ie).
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The second objective aperture on JEOL microscopes with an ultra high resolution pole-piece is actually not below the lens, but is introduced through a hole drilled in the lower pole piece, substantially below the gap.
A. John Mardinly, Ph.D., P.E.
} On Jan 9, 2019, at 4:45 PM, Phil.Ahrenkiel-at-sdsmt.edu wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.com_MicroscopyListserver&d=DwIBAg&c=l45AxH-kUV29SRQusp9vYR0n1GycN4_2jInuKy6zbqQ&r=VLPJ8OE-c_C6joGeE1ftlvxMmQPq9N6mpKZONBRt90E&m=W6eM-3dTJ7K8KaqJ7YGk_RyEiyzil-bCp99PoJGDg-U&s=Wkw4zGKdXd5EvzLrjYnTIdyMvtQG3ziwgjAflAmS__Y&e= } On-Line Help https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.com_MicroscopyListserver_FAQ.html&d=DwIBAg&c=l45AxH-kUV29SRQusp9vYR0n1GycN4_2jInuKy6zbqQ&r=VLPJ8OE-c_C6joGeE1ftlvxMmQPq9N6mpKZONBRt90E&m=W6eM-3dTJ7K8KaqJ7YGk_RyEiyzil-bCp99PoJGDg-U&s=z9T3ciafx2nXamEUVHaRA9rZWu6gXurtEMpglti3lvI&e= } ---------------------------------------------------------------------------- } } The OL is essentially an immersion lens; focusing occurs over an extended region between the top and bottom pole pieces that contains the specimen. The top pole piece contributes to forming parallel illumination on the specimen, and a crossover occurs above the lower pole piece, so an objective aperture can be situated below the specimen plane, but above the lower pole piece. A second crossover occurs below the OL, so our JEOL has apertures both within the lens and below the lens. } } I wish the apertures were closer to the true back focal plane(s), though. They tend to limit the field of view at low magnifications. } } -Phil } -----Original Message----- } X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} } Sent: Tuesday, January 8, 2019 4:29 PM } To: Ahrenkiel, Phil {Phil.Ahrenkiel-at-sdsmt.edu} } Subject: [EXT] [Microscopy] viaWWW:TEM apertures } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.com_MicroscopyListserver&d=DwIBAg&c=l45AxH-kUV29SRQusp9vYR0n1GycN4_2jInuKy6zbqQ&r=VLPJ8OE-c_C6joGeE1ftlvxMmQPq9N6mpKZONBRt90E&m=W6eM-3dTJ7K8KaqJ7YGk_RyEiyzil-bCp99PoJGDg-U&s=Wkw4zGKdXd5EvzLrjYnTIdyMvtQG3ziwgjAflAmS__Y&e= } On-Line Help https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.com_MicroscopyListserver_FAQ.html&d=DwIBAg&c=l45AxH-kUV29SRQusp9vYR0n1GycN4_2jInuKy6zbqQ&r=VLPJ8OE-c_C6joGeE1ftlvxMmQPq9N6mpKZONBRt90E&m=W6eM-3dTJ7K8KaqJ7YGk_RyEiyzil-bCp99PoJGDg-U&s=z9T3ciafx2nXamEUVHaRA9rZWu6gXurtEMpglti3lvI&e= } ---------------------------------------------------------------------------- } } } } } -------- Forwarded Message -------- } } } X-from: roger.ristau-at-uconn.edu } } } This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.com_MLFormMail.html&d=DwIBAg&c=l45AxH-kUV29SRQusp9vYR0n1GycN4_2jInuKy6zbqQ&r=VLPJ8OE-c_C6joGeE1ftlvxMmQPq9N6mpKZONBRt90E&m=W6eM-3dTJ7K8KaqJ7YGk_RyEiyzil-bCp99PoJGDg-U&s=Hq9CyOPyBmg97G5ClaRX4_LcZ5Ui3u18R0PCu05mXVc&e= } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy to both } roger.ristau-at-uconn.edu, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers } can benefit from our collective wisdom } --------------------------------------------------------------------------- } } Email: roger.ristau-at-uconn.edu Name: Roger Ristau } } Organization: University of Connecticut } } Title-Subject: [Filtered] TEM apertures } } Message: While describing the operation of the TEM to students, I note that all the conventional textbooks depict the objective aperture as below the objective lens, that is, they show the sequence in ray diagrams as: specimen, lower objective lens, objective aperture coincident with the back focal plane. The problem is that the objective aperture is phycically located above the lower objective lens in our TEM: i.e. specimen, objective aperture, lower objective lens. } The thing I can't get my head around is how the back focal plane can be coincident with the objective lens in the second case? Or is this a case of a virtual aperture? } } Confused in Connecticut } } Login Host: 137.99.158.135 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 16, 53 -- From microscopy.listserver-at-gmail.com Tue Jan 8 17:24:40 2019 16, 53 -- Received: from mail-io1-f50.google.com (mail-io1-f50.google.com [209.85.166.50]) } 16, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x08NOe3u022178 } 16, 53 -- for {microscopy-at-microscopy.com} ; Tue, 8 Jan 2019 17:24:40 -0600 } 16, 53 -- Received: by mail-io1-f50.google.com with SMTP id c2so4555803iom.12 } 16, 53 -- for {microscopy-at-microscopy.com} ; Tue, 08 Jan 2019 15:31:02 -0800 (PST) } 16, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 16, 53 -- d=gmail.com; s=20161025; } 16, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 16, 53 -- :in-reply-to:content-language:content-transfer-encoding; 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Email: mike.toalson-at-elementpi.com Name: Mike Toalson
Organization: element Pi, LLC
Title-Subject: [Filtered] TEM sample for Outreach
Message: Would anyone be willing to donate a few prepared TEM samples like interesting tissue or bacteria or other cellular specimens ready for analysis? These would be used for educational outreach at the high school and undergrad level. They would need to work with STEM mode in a 30kV max SEM so tissue sections would need to be fairly thin.
Or does anyone know where you can buy such sample standards? None of the EM consumable suppliers seem to carry anything like this.
Any questions or to provide a few samples, please contact me.
Much Appreciated!!
Mike Toalson element Pi, LLC 833-314-1593
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does anyone have the manuals "A", "B" and "C" (user-manuals and alignment procedures) - preferably in German language or even electronic schematics of the ZEISS EM 109 transmission electron microscope available in PDF or can copy the manuals ? I would happily compensate for the costs...
Best wishes,
Stefan
--
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both mastopicalconference-at-gmail.com, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Title-Subject: [Filtered] QMA 2019 abstract submission open
Message: Hello
Abstract submission for Quantitative Microanalysis 2019 is open! QMA 2019 is an MAS Topical Conference focused on topics related to quantitative microanalysis by wavelength-dispersive (WDS) and energy-dispersive (EDS) spectrometry.
The conference will be held June 24-27, 2019 at the University of Minnesota, Minneapolis. For a list of programmatic topics, registration, abstract template, and submission please visit our website: https://the-mas.org/events/topical-conferences/qma-2019/
We look forward to your contribution, QMA 2019 Organizing Committee
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We need a new stereomicroscope and I'm not current on what's available. Anyone with a recommendation?
I'd like: Apochromatic lenses, Trans illuminated base, Trinocular head and camera, something with good color and resolution, Computer software with easy file tracking, image processing, measurements and annotation, Magnification range from about 1 to100-ish X (with high NA objectives, of course).
We'd like if possible, fine focus as well as coarse, flat field, good mechanical stability.
I feel a little like that commercial where the neighbor ask his buddy to do all the leg work to hire a contractor. Sorry.
I'm very much open to responses from dealers.
Thanks in advance!
Stay safe...........
Frank Karl Microscopist Akron Rubber Development Laboratory 2887 Gilchrist Road Akron, Ohio 44305
Check out Mager Scientific - Nikon http://www.magersci.com/nikonmicroscopes/ and Nusbaum - Leica https://nuhsbaum.com/nuhsbaum-products/
They're the dealers for Nikon & Leica your (our) area, and both have what you're looking for. There are others, like Olympus https://www.olympus-lifescience.com/en/ and Zeiss, distributed in the Midwest by Lukas lukasmicroscope.com
Plus industrial inspection stereoscopes, but I don't have URLs handy for those.
Phil ------------- Philip Oshel Imaging Facility Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 989 774-3576 office 989 774-7567 lab
I always check to see what BidService has in pre-owned stereo scopes.
Get Outlook for iOS {https://aka.ms/o0ukef} ---------------------------------------------------------------------------------------------------- *From:* microscopy.listserver-at-gmail.com *Sent:* Wednesday, January 16, 2019 08:41 *To:* Basgall,Edward *Subject:* [Microscopy] Fwd: needs a new Stereomicroscope.................
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We need a new stereomicroscope and I'm not current on what's available. Anyone with a recommendation?
I'd like: Apochromatic lenses, Trans illuminated base, Trinocular head and camera, something with good color and resolution, Computer software with easy file tracking, image processing, measurements and annotation, Magnification range from about 1 to100-ish X (with high NA objectives, of course).
We'd like if possible, fine focus as well as coarse, flat field, good mechanical stability.
I feel a little like that commercial where the neighbor ask his buddy to do all the leg work to hire a contractor. Sorry.
I'm very much open to responses from dealers.
Thanks in advance!
Stay safe...........
Frank Karl Microscopist Akron Rubber Development Laboratory 2887 Gilchrist Road Akron, Ohio 44305
Hi Frank, If you want a branded one, get a quote from your local microscope sales rep (Leica, Olympus, Nikon or Carl Zeiss or other good companies which I would have missed out). You can google the company names and find the local reps contact in your area. There are other microscope dealers (like http://precisionmicroscopesales.com/index.php?route=product/category&path=59_63). With a few modifications and attachments, you can convert your bright field stereo microscope into a fluorescence microscope (eg. http://www.nightsea.com/. If you are not bothered too much on image resolution). Depends on what you are looking for. There is also the Echo Revolve (https://discover-echo.com/revolve) where you can use them as upright and inverted with bright field and fluorescence capability. You can also check out the adapters for microscope where you can convert a simple microscope into digital using your cell phone camera - https://novagrade.com/shop/phone-adapter-microscope-edition/ (Resolution is the criteria again). The range and price is so wide, it should be decided by what you want the microscope for. Ask the vendors to bring in and show how it can be applied for your use (You will be surprised by some of the features including the software - make sure if the images generated using the microscope software can be opened and annotated using other software and the cost for the full version - if a free version is available, support, etc.). No commercial interest and the order of the companies is nothing to do with the quality or my preference - I love all the microscopes from any vendor.
Good luck
Sathya Srinivasan Director Imaging and Morphology Support Core ONPRC Beaverton, OR 97006
On Wed, Jan 16, 2019 at 5:42 AM {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote:
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X-from: Frank Karl {frank_karl-at-ardl.com {mailto:frank_karl-at-ardl.com} }
We need a new stereomicroscope and I'm not current on what's available. Anyone with a recommendation?
I'd like: Apochromatic lenses, Trans illuminated base, Trinocular head and camera, something with good color and resolution, Computer software with easy file tracking, image processing, measurements and annotation, Magnification range from about 1 to100-ish X (with high NA objectives, of course).
We'd like if possible, fine focus as well as coarse, flat field, good mechanical stability.
I feel a little like that commercial where the neighbor ask his buddy to do all the leg work to hire a contractor. Sorry.
I'm very much open to responses from dealers.
Thanks in advance!
Stay safe...........
Frank Karl Microscopist Akron Rubber Development Laboratory 2887 Gilchrist Road Akron, Ohio 44305
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Email: mbrazil-at-vergason.com Name: Michael Brazil
Message: I have recently acquired a Hitachi S-570 SEM (1984 vintage). Scope works well, however it arrived missing the holder for the fixed objective aperture. Hitachi service does not offer these parts. If anyone is parting out a similar model, I'm a customer. If anyone has a such a scope, I would be grateful for careful measurements that would allow a machine shop to duplicate the holder.
Thanks,
Michael Brazil Senior Scientist Vergason Technology mbrazil-at-vergason.com
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Title-Subject: [Filtered] Paper submission for M&M 2019 Symposium P10: Microscopy and microanalysis techniques in characterizing natural and synthetic materials
Message: Dear colleagues, We would like to draw your attention to the following M&M 2019 session, August 4-8, 2019, Portland, Oregon: The submission portal is open! Submit your 2-page paper as soon as possible at https://www.microscopy.org/MandM/2019/. The deadline is Friday, February 15. M&M 2019 Symposium P10: Applications of integrated electron probe microscopy and microanalysis techniques in characterizing natural and synthetic materials
Description: Electron microbeam techniques, such as SEM/ESEM, EPMA and TEM/STEM, use a focused electron probe or a small parallel electron beam to bombard a specimen and generate signals at a scale from micrometer down to Angstrom level. These signals include secondary electron (SE), backscattered electron (BSE), characteristic X-ray, cathodoluminescence (CL), transmitted electron, diffracted or scattered electron, etc. Information acquired using these signals includes image, chemistry and crystal structure of a specimen at micrometer, nanometer and sub-Angstrom levels. We welcome contributions covering the entire range of electron probe microscopy and microanalysis of natural and synthetic materials, such as: • Imaging from SE, BSE, X-ray, CL, charge contrast, transmitted electron, diffracted or scattered electron, etc. • Qualitative and quantitative determination of chemistry of natural and synthetic materials • Repeatability, reproducibility and compatibility of quantitative microanalysis standards • Crystal structure determination using electron diffraction or electron backscattered diffraction (EBSD)
Feel free to share this information to other potentially interested colleagues. We are very much looking forward to seeing you in Portland, Oregon! Best Regards,
Organizers:
Donggao Zhao Email: zhaodo-at-umkc.edu Affiliation: University of Missouri-Kansas City Phone 1: 816-235-2072 (W) Phone 2: 803-732-6280 (M)
Minghua Ren Email: minghua.ren-at-unlv.edu Affiliation: University of Nevada, Las Vegas Phone 1: 702-774-1465 (W) Phone 2: 915-217-4392 (M)
Owen Neill Email: okneill-at-umich.edu Affiliation: University of Michigan Phone 1: 734-615-6657 (W) Phone 2: (207) 653-6331 (M)
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Email: fei.long-at-queensu.ca Name: Fei Long
Organization: Queen's University
Title-Subject: [Filtered] EDS mapping on bacteria cell embedded in epoxy resin
Message: I am trying to do EDS elemental analysis on microtome sesctioned bacteria cells embedded in epoxy resion with TEM at 200kV in STEM mode. However, the electron beam burn the sample instantly. Does anyone have good experience in doing chemical analysis on biology samples with TEM by EDX? Is there any irradiation resistant epoxy that I can use for the bacteria embedding? Lastly, if I cool the sample to low temperatures with liquid nitrogen by using a cooling holder in TEM, does it help to reduce the beam burning?
Any suggestions will be much appreciated!
Best,
Fei
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X-from: Gemming, Dr. Thomas {T.Gemming-at-ifw-dresden.de}
Dear Representatives of the (Electron) Microscopy Societies and otherwise involved microscopists,
please find attached the announcement of the Harald Rose Distinguished Lecture 2019 award of the German Society for Electron Microscopy. Please distribute the announcement within your society and interested colleagues. The application deadline is February 28th. The distinguished lecture will take place at the microscopy conference MC2019 in Berlin in the first week of September 2019.
Best regards and thank you for your help in spreading this announcement,
Thomas Gemming Executive secretary of the German Society for Electron Microscopy
Dr. Thomas Gemming Act. Director of Institute for Complex Materials Leibniz-Institut für Festkörper- und Werkstoffforschung Dresden e.V. Helmholtzstr. 20, 01069 Dresden
In my experience, most of the epoxy resins designed for (ultra)microtomy are reasonably durable under electron beams, even up to 300kV. You didn't mention whether you are using a FEG or thermionic microscope, so I'm not exactly sure what mechanism might be causing the damage you are experiencing. With thermionic microscopes, you probably don't have enough current density in STEM mode to literally drill through your sample and most of the damage you are seeing is likely from charging. Coating your sample with a few angstroms or a nanometer of carbon can completely eliminate the charging and stabilize the sample. Sometimes you need to coat both sides with a particularly ornery sample.
For a FEG, you can easily have enough current density in STEM mode to sputter through your sample. You'll need to adjust your condenser illumination conditions and condenser aperture to reduce current density. If you're using reasonable settings that have worked well with previous similar samples, then the problem is probably charging. A small coating of carbon, as mentioned above, can eliminate that issue.
It's still possible that the epoxy used is not one recommended for electron microscopy. I'll let the more experienced microtomy and biological TEM people address those concerns.
Good luck, Chris
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However, the electron beam burn the sample instantly. } Does anyone have good experience in doing chemical analysis on biology samples with TEM by EDX? Is } there any irradiation resistant epoxy that I can use for the bacteria embedding? Lastly, if I cool } the sample to low temperatures with liquid nitrogen by using a cooling holder in TEM, does it help } to reduce the beam burning? } } Any suggestions will be much appreciated! } } Best, } } Fei } } Login Host: 142.169.78.78 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 18, 53 -- From microscopy.listserver-at-gmail.com Fri Jan 18 05:10:13 2019 } 18, 53 -- Received: from mail-it1-f180.google.com (mail-it1-f180.google.com [209.85.166.180]) } 18, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x0IBADcB031294 } 18, 53 -- for {microscopy-at-microscopy.com} ; Fri, 18 Jan 2019 05:10:13 -0600 } 18, 53 -- Received: by mail-it1-f180.google.com with SMTP id c9so5314199itj.1 } 18, 53 -- for {microscopy-at-microscopy.com} ; Fri, 18 Jan 2019 03:17:05 -0800 (PST) } 18, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 18, 53 -- d=gmail.com; s=20161025; } 18, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 18, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 18, 53 -- bh=kSD2ndbREtoIAQZeG2abLeONM2tXrOIGKga0bCBN7lU=; } 18, 53 -- b=UhJ/kCzE8QzrtIGTfbRWv4NrNapDTlAa6Q1klhMGhuyQwakB2vXREqJpkhspQ9N+C5 } 18, 53 -- o34bWQSo/NdJg4Myjq+QhwW2XsimDiBFZ+y0YQQFEB9THQ1vJFebT506k+W5Vk+wZiV6 } 18, 53 -- 5j+x71oAAGZDT+1ktueGWL1Lp5ks2+SwhDquAw1jQhaCUF0a5UgmyBXfsA66awxx7P/X } 18, 53 -- moBXdHFxN9pKYXuBd3Ljs4JPj1mAz+WcgSQWWEUgYpmIYyF39ZdLk344m/DDM8sJBABY } 18, 53 -- xv5+pWDUwP4bLnZ/bi7v79zmDheCh9EXVBmBq3ocQvcKni0bse5ZxpF+CFWJVPpSp1wy } 18, 53 -- y6XA== } 18, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 18, 53 -- d=1e100.net; s=20161025; } 18, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 18, 53 -- :user-agent:mime-version:in-reply-to:content-language } 18, 53 -- :content-transfer-encoding; } 18, 53 -- bh=kSD2ndbREtoIAQZeG2abLeONM2tXrOIGKga0bCBN7lU=; } 18, 53 -- b=LyyxNkRYgA9RiunM2zoO6pSnLodrb5AbHENA+NaEDALSk1uZ6HS4NdrG0KXmIsIRIk } 18, 53 -- CsMZ22ZkAMRjgflK5iKFBRRKrJysbes8CulRiJP54lmtu/4+/1PptC+BFjZACrUwU2ce } 18, 53 -- aMykM/UQLEJgN01e1d8l51KX3ZMdIeqhv7jrQ2JhPBgqnbPAUtZAmaI3IiRAlMLNVv/q } 18, 53 -- 8p5dZNYNUl+JBwvbz7hScPezRyTkxLz8ukQEOTevNY4IU6j8jmyH42Szk/LU0qPd+xoo } 18, 53 -- /OsNxCA7aayd0ken3qe5sFawwQ1ggzzGOfNIWtRPehEB9hcziO8gGCPinkrhNhf7hKMb } 18, 53 -- 9AHg== } 18, 53 -- X-Gm-Message-State: AJcUukesDR3H4Azq86Q7ASphVAxJxpUkly2bntB7SmccRWxsxgD2GIIL } 18, 53 -- 6l3IRFEsEZyhZKjJyK247oE3jSw0 } 18, 53 -- X-Google-Smtp-Source: ALg8bN7yTRcED2iYff7BkMsp65NgdvaJo16coTp/KM7z8xYJvO/mBwTx8b7QzhxG3Dhcc8mt9e4a4w== } 18, 53 -- X-Received: by 2002:a24:70d2:: with SMTP id f201mr10395381itc.127.1547810225381; } 18, 53 -- Fri, 18 Jan 2019 03:17:05 -0800 (PST) } 18, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:595a:5ca1:6ede:1a88]) } 18, 53 -- by smtp.googlemail.com with ESMTPSA id m7sm1840209itk.38.2019.01.18.03.17.04 } 18, 53 -- for {microscopy-at-microscopy.com} } 18, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 18, 53 -- Fri, 18 Jan 2019 03:17:04 -0800 (PST) } 18, 53 -- Subject: viaWWW:EDS mapping on bacteria cell embedded in epoxy resin } 18, 53 -- References: {201901171753.x0HHrEfF027061-at-microscopy.com} } 18, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 18, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 18, 53 -- X-Forwarded-Message-Id: {201901171753.x0HHrEfF027061-at-microscopy.com} } 18, 53 -- Message-ID: {b710e902-a2d2-f6bf-cc6f-0c0a20a8bf88-at-gmail.com} } 18, 53 -- Date: Fri, 18 Jan 2019 05:17:03 -0600 } 18, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.14; rv:60.0) } 18, 53 -- Gecko/20100101 Thunderbird/60.4.0 } 18, 53 -- MIME-Version: 1.0 } 18, 53 -- In-Reply-To: {201901171753.x0HHrEfF027061-at-microscopy.com} } 18, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 18, 53 -- Content-Language: en-US } 18, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
From ralpzand8wo-at-gmail.com Fri Jan 18 10:47:23 2019 Return-Path: {ralpzand8wo-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.161.148] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x0IGlLvY004678 for {microscopylistserverarchive7-at-microscopy.com} ; Fri, 18 Jan 2019 10:47:22 -0600 Received: from [134.209.42.110] by mxs.perenter.com with NNFMP; Fri, 18 Jan 2019 14:43:22 -0200 Received: from unknown (148.244.134.221) by snmp.otwaloow.com with SMTP; Fri, 18 Jan 2019 14:23:46 -0200 Message-ID: {9660C8C9.D142AF3B-at-gmail.com}
Fei; There are several ways to reduce damage, but they come with reduced spatial resolution: 1) Reduce beam current (Spot size) and/or slightly defocus the beam. You will have less signal, requiring longer acquisition time and drift will be a factor. 2) Use a thicker section. Due to beam spreading, resolution may be reduced. 3) Cooling. Cold stages have inherently more drift. 4) Sputter a carbon or aluminum coating on the EXIT surface of the specimen. That will reduce charging and conduct heat away from the beam spot but will add some background to the spectra. 5) Some combination of the previous 4.
A. John Mardinly, Ph.D., P.E.
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However, the electron beam burn the sample instantly. } Does anyone have good experience in doing chemical analysis on biology samples with TEM by EDX? Is } there any irradiation resistant epoxy that I can use for the bacteria embedding? Lastly, if I cool } the sample to low temperatures with liquid nitrogen by using a cooling holder in TEM, does it help } to reduce the beam burning? } } Any suggestions will be much appreciated! } } Best, } } Fei } } Login Host: 142.169.78.78 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 18, 53 -- From microscopy.listserver-at-gmail.com Fri Jan 18 05:10:13 2019 } 18, 53 -- Received: from mail-it1-f180.google.com (mail-it1-f180.google.com [209.85.166.180]) } 18, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x0IBADcB031294 } 18, 53 -- for {microscopy-at-microscopy.com} ; Fri, 18 Jan 2019 05:10:13 -0600 } 18, 53 -- Received: by mail-it1-f180.google.com with SMTP id c9so5314199itj.1 } 18, 53 -- for {microscopy-at-microscopy.com} ; Fri, 18 Jan 2019 03:17:05 -0800 (PST) } 18, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 18, 53 -- d=gmail.com; s=20161025; } 18, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 18, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 18, 53 -- bh=kSD2ndbREtoIAQZeG2abLeONM2tXrOIGKga0bCBN7lU=; } 18, 53 -- b=UhJ/kCzE8QzrtIGTfbRWv4NrNapDTlAa6Q1klhMGhuyQwakB2vXREqJpkhspQ9N+C5 } 18, 53 -- o34bWQSo/NdJg4Myjq+QhwW2XsimDiBFZ+y0YQQFEB9THQ1vJFebT506k+W5Vk+wZiV6 } 18, 53 -- 5j+x71oAAGZDT+1ktueGWL1Lp5ks2+SwhDquAw1jQhaCUF0a5UgmyBXfsA66awxx7P/X } 18, 53 -- moBXdHFxN9pKYXuBd3Ljs4JPj1mAz+WcgSQWWEUgYpmIYyF39ZdLk344m/DDM8sJBABY } 18, 53 -- xv5+pWDUwP4bLnZ/bi7v79zmDheCh9EXVBmBq3ocQvcKni0bse5ZxpF+CFWJVPpSp1wy } 18, 53 -- y6XA== } 18, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 18, 53 -- d=1e100.net; s=20161025; } 18, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 18, 53 -- :user-agent:mime-version:in-reply-to:content-language } 18, 53 -- :content-transfer-encoding; } 18, 53 -- bh=kSD2ndbREtoIAQZeG2abLeONM2tXrOIGKga0bCBN7lU=; } 18, 53 -- b=LyyxNkRYgA9RiunM2zoO6pSnLodrb5AbHENA+NaEDALSk1uZ6HS4NdrG0KXmIsIRIk } 18, 53 -- CsMZ22ZkAMRjgflK5iKFBRRKrJysbes8CulRiJP54lmtu/4+/1PptC+BFjZACrUwU2ce } 18, 53 -- aMykM/UQLEJgN01e1d8l51KX3ZMdIeqhv7jrQ2JhPBgqnbPAUtZAmaI3IiRAlMLNVv/q } 18, 53 -- 8p5dZNYNUl+JBwvbz7hScPezRyTkxLz8ukQEOTevNY4IU6j8jmyH42Szk/LU0qPd+xoo } 18, 53 -- /OsNxCA7aayd0ken3qe5sFawwQ1ggzzGOfNIWtRPehEB9hcziO8gGCPinkrhNhf7hKMb } 18, 53 -- 9AHg== } 18, 53 -- X-Gm-Message-State: AJcUukesDR3H4Azq86Q7ASphVAxJxpUkly2bntB7SmccRWxsxgD2GIIL } 18, 53 -- 6l3IRFEsEZyhZKjJyK247oE3jSw0 } 18, 53 -- X-Google-Smtp-Source: ALg8bN7yTRcED2iYff7BkMsp65NgdvaJo16coTp/KM7z8xYJvO/mBwTx8b7QzhxG3Dhcc8mt9e4a4w== } 18, 53 -- X-Received: by 2002:a24:70d2:: with SMTP id f201mr10395381itc.127.1547810225381; } 18, 53 -- Fri, 18 Jan 2019 03:17:05 -0800 (PST) } 18, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:595a:5ca1:6ede:1a88]) } 18, 53 -- by smtp.googlemail.com with ESMTPSA id m7sm1840209itk.38.2019.01.18.03.17.04 } 18, 53 -- for {microscopy-at-microscopy.com} } 18, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 18, 53 -- Fri, 18 Jan 2019 03:17:04 -0800 (PST) } 18, 53 -- Subject: viaWWW:EDS mapping on bacteria cell embedded in epoxy resin } 18, 53 -- References: {201901171753.x0HHrEfF027061-at-microscopy.com} } 18, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 18, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 18, 53 -- X-Forwarded-Message-Id: {201901171753.x0HHrEfF027061-at-microscopy.com} } 18, 53 -- Message-ID: {b710e902-a2d2-f6bf-cc6f-0c0a20a8bf88-at-gmail.com} } 18, 53 -- Date: Fri, 18 Jan 2019 05:17:03 -0600 } 18, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.14; rv:60.0) } 18, 53 -- Gecko/20100101 Thunderbird/60.4.0 } 18, 53 -- MIME-Version: 1.0 } 18, 53 -- In-Reply-To: {201901171753.x0HHrEfF027061-at-microscopy.com} } 18, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 18, 53 -- Content-Language: en-US } 18, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
From geracore539w-at-gmail.com Tue Jan 22 07:22:23 2019 Return-Path: {geracore539w-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.161.139] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x0MDMLuP003112 for {microscopylistserverarchive7-at-microscopy.com} ; Tue, 22 Jan 2019 07:22:22 -0600 Received: from unknown (HELO mmx09.tilkbans.com) (Tue, 22 Jan 2019 15:17:07 +0200) by mts.locks.grgtween.net with QMQP; Tue, 22 Jan 2019 15:17:07 +0200 Received: from m1.gns.snv.thisdomainl.com [28.15.174.207] by relay.2yahoo.com with QMQP; Tue, 22 Jan 2019 14:58:16 +0200 Received: from snmp.otwaloow.com ([121.145.169.67]) by mmx09.tilkbans.com with QMQP; Tue, 22 Jan 2019 14:45:57 +0200 Message-ID: {5e7001d4b25e$71ac1540$6f55cd10-at-geracore539w} Reply-To: "Iliana Isham " {geracore539w-at-gmail.com}
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X-from: jennifer.cookman-at-ul.ie
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Email: jennifer.cookman-at-ul.ie Name: Jennifer
Organization: University of Limerick
Title-Subject: [Filtered] Installing GMS3 on Mac?
Message: Dear All,
I want to ask the community if anyone has tried to installed GMS3 on a Mac?
I have tried to use wine and brew to install the right components but GMS3 seems to be incompatible with Windows XP which is what wine is simulating.
Any help on this would be great! I do need the in situ portion of GMS3!
Best,
Jenn
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both elena.belluso-at-unito.it, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Email: elena.belluso-at-unito.it Name: Elena Belluso
Organization: I-University of Torino -Dept of Earth Sciences and "G. Scansetti" Interdepartmental Center for Studies on Asbestos and other Toxic Particulates,
Title-Subject: [Filtered] FROM ATOMIC- TO MICRO-SCALE PROCESSES: call for abstracts
Message: Dear Colleague, We are pleased to invite you to submit an abstract for an oral presentation (or a poster) to the session 4g “MINERAL TRANSFORMATIONS IN MEDICAL AND ENVIRONMENTAL MINERALOGY: FROM ATOMIC- TO MICRO-SCALE PROCESSES” at Goldschmidt 2019, the international conference on geochemistry and related subjects, scheduled from 18 to 23 August 2019 in Barcelona (E). The key-note speaker of the session is Mihály Pósfai (University of Pannonia).
The purpose of this session is to spread the more recent investigations dealing with the relationship between minerals, environment, and human body.
This topic includes the study of naturally occurring minerals, their transformation in the environment and within the human body, and minerals synthesized by living organism themselves.
Thus, the session covers a broad interdisciplinary field positioned between geology, mineralogy, geochemistry, material science, and medicine.
Particular attention will be dedicated (but not limited) to contributions investigating the synthesis, transformation and function of minerals in the environment, in living organisms, and those minerals with an active role in human disease development.
This session welcomes contributions related to the study and the comprehension of the complex relationships occurring between natural or anthropogenic environments and different minerals.
Among various targets, this session aims to present the state-of-the-art in this multifaceted and complex topic and to develop interdisciplinary collaborations.
Note that the abstract deadline is March 29th (23:59 CET) (https://goldschmidt.info/2019/abstracts/instructions#who) We look forward to seeing you in Barcelona in August! With ours best wishes for the New Year, the convenors Elena Belluso (elena.belluso-at-unito.it) and Ruggero Vigliaturo (ruggero-at-sas.upenn.edu) PS The Goldschmidt Grant Program provides waived registration fees and travel support for early career delegates residents of specific countries and for US students. See at https://goldschmidt.info/2019/grants for details.
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I'm working with a student who has metal samples with a ~1um thick layer of amorphous silicon oxynitride on the surface, and they want to prepare cross section TEM samples. We have tried gluing the sample sandwich together with Epo-Tek 353ND (also known as Gatan G1) and MBond 610, and both stacks fell apart on cutting.
I assumed that the SiON layers were delaminating, but a visual inspection using the optical microscope convinced me this was not likely the underlying cause of the problem. Both epoxies used are not expired and were cured according to the manufacturer's cure schedule. I have had no issues with cross sections of other sample systems and these exact same epoxies, so I wonder if we need to use a different epoxy chemistry than these standards. Does anyone have experience with the SiON sample system? Is there a better epoxy to use, like Araldite? We would like to try to create a sample stack before giving up and switching to attempting tripod/wedge polishing.
We have launched a new online course on Transmission Electron Microscopy for Materials Science, by EPFL on the Coursera platform:
https://www.coursera.org/learn/microscopy
In this course, we provide a comprehensive introduction to TEM theory, including: - the instrument and its optics - the basics of electron diffraction and dynamical scattering - the theory of phase contrast imaging.
Built from the ground up, the course is intended to be a starting point for understanding and working with TEM; to be used either as a stand alone resource, or in complement to books and in person teaching or coaching. We hope that it proves useful to our electron microscopy community.
Course registration is open to all and can be made at any time. For any questions about the course either PM me directly or use the course forums.
With regards Duncan Alexander
EPFL-SB-IPHYS-LSME
==============================Original Headers============================== 7, 32 -- From duncan.alexander-at-epfl.ch Wed Jan 23 13:54:38 2019 7, 32 -- Received: from smtp4.epfl.ch (smtp4.epfl.ch [128.178.224.219]) 7, 32 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x0NJsbZG013482 7, 32 -- for {microscopy-at-microscopy.com} ; Wed, 23 Jan 2019 13:54:38 -0600 7, 32 -- Received: (qmail 38610 invoked by uid 107); 23 Jan 2019 20:01:47 -0000 7, 32 -- Received: from ax-snat-224-180.epfl.ch (HELO ewa09.intranet.epfl.ch) (192.168.224.180) (TLS, AES256-GCM-SHA384 cipher) 7, 32 -- by mail.epfl.ch (AngelmatoPhylax SMTP proxy) with ESMTPS; Wed, 23 Jan 2019 21:01:47 +0100 7, 32 -- X-EPFL-Auth: /3zp2ZrSb6IPNQtEl7KkU+xUyuefjgDRcQWFfu9GjVQE/X4LVcg= 7, 32 -- Received: from ewa01.intranet.epfl.ch (128.178.224.158) by 7, 32 -- ewa09.intranet.epfl.ch (128.178.224.180) with Microsoft SMTP Server 7, 32 -- (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_128_GCM_SHA256) id 7, 32 -- 15.1.1591.10; Wed, 23 Jan 2019 21:01:46 +0100 7, 32 -- Received: from ewa01.intranet.epfl.ch ([fe80::5832:f4e:2e6b:15dc]) by 7, 32 -- ewa01.intranet.epfl.ch ([fe80::5832:f4e:2e6b:15dc%3]) with mapi id 7, 32 -- 15.01.1591.008; Wed, 23 Jan 2019 21:01:46 +0100 7, 32 -- From: Duncan Alexander {duncan.alexander-at-epfl.ch} 7, 32 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 7, 32 -- Subject: New online course on TEM 7, 32 -- Thread-Topic: New online course on TEM 7, 32 -- Thread-Index: AQHUs1Z4adLvyLUr4UqqoVyzkEsGoQ== 7, 32 -- Date: Wed, 23 Jan 2019 20:01:46 +0000 7, 32 -- Message-ID: {90B865DD-AB5E-4C5C-AA97-4AF469F124A8-at-epfl.ch} 7, 32 -- Accept-Language: en-GB, fr-CH, en-US 7, 32 -- Content-Language: en-US 7, 32 -- X-MS-Has-Attach: 7, 32 -- X-MS-TNEF-Correlator: 7, 32 -- x-originating-ip: [80.218.92.125] 7, 32 -- Content-Type: text/plain; charset="us-ascii" 7, 32 -- Content-ID: {6FF1CB06B1AC28469B1694AA96FA48DA-at-intranet.epfl.ch} 7, 32 -- MIME-Version: 1.0 7, 32 -- Content-Transfer-Encoding: 8bit 7, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id x0NJsbZG013482 ==============================End of - Headers==============================
Please consider submitting an abstract for our session on Quantitative Microscopy in Earth, Archaeological and Planetary Sciences at the Microscience Microscopy Congress (mmc2019) on July 4th 2019 in Manchester, UK. The deadline for submission is February 15th and we welcome contributions from as wide a field as possible in these disciplines.
Session description ******************************************************************* Quantitative microscopic investigation is critical for advancing our understanding of scientific problems today. Researchers have access to a broad range of analytical equipment including, but not limited to, light, electron and gas ion microscopy, secondary ion mass spectrometry, 3-D correlative microscopy, X-ray tomography and associated spectroscopic techniques. We invite presentations on examples from Earth, Planetary and Archaeological Sciences where novel microscopy techniques have been used to help construct quantitative datasets preferably with practical outcomes and applications which advance scientific and/or technical understanding.
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Email: gcanzalo-at-mtu.edu Name: Jerry Anzalone
Organization: Michigan Tech
Title-Subject: [Filtered] Aberrations: Electron Optics
Message: Good day, Microscopists:
I'm developing an online SEM demonstrator/simulator as an instructional aid and have come to realize I know next to nothing about electron optics. Goldstein, et al., and others, make the categorical claim that minimizing the working distance yields a probe least affected by lens aberrations, yet the equation for the diameter of the spherical aberration disk of least confusion indicates the diameter increases as the cube of convergence angle. What am I missing?
While I understand the interaction volume is order(s) of magnitude larger than the beam, largely obviating the utility of calculating the effects of aberrations on probe diameter, I nonetheless wish to produce an accurate simulation, at least within an order of magnitude. Any assistance, including papers (that don't include triple integrals) is much appreciated. Ikea-like drawings might be best.
Cheers and thanks,
Jerry
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Topics Include: Electron Microscopy, Cryo-SIMS and SIMS Imaging, NanoSIMS, Atom Probe Tomography and Cryo-Spectroscopies
Chairs: Dr Paula Rakowska (National Physical Laboratory, UK) and Dr Kirsty MacLellan-Gibson (National Institute for Biological Standards and Control, UK).
Best Wishes,
Dr Anwen Bullen (on behalf of Dr MacLellan-Gibson)
I have summarized the replies I received regarding this issue below:
1. "Since the SiON is ~1umthick, top monolayers probably do not matter. I can suggest to try the following : 1. Plasma clean the sample just before applying epoxy. We are using 4-5 min of pure Ar at 50W forward RF with range 5W in our GATAN Solarus plasma cleaner. or 2. Sputter ~1-2 nm of Cr or Fe on the top surface before applying epoxy. We use GATAN PECS to do that. Sputtering with any reactive metal available in your lab, if Fe or Cr targets are not available, should also work."
2. Many are concerned the film is delaminating from the metal substrate. We should probably check this using the SEM, but we don't see any evidence of delamination in the optical microscope (when comparing failed glue specimen with pristine specimen).
3. Many suggested FIB. The student prefers a conventional xtem sample preparation to generate a larger electron transparent region than the FIB would provide, but if all else fails then we can fall back to the FIB.
4. "A simple suggestion that, perhaps, might work (this is how we work): Why don’t you inverse the order of operations? I mean: first, cut the small pieces and then, glue them as a sandwich. Hopefully, the glued pieces will hold together during the grinding steps. Good luck with the ion milling, afterwards."
5. "I have used Devcon 5-minute epoxy for many years on all sorts of samples with success. It is much more viscous than the other usual TEM epoxies but, you know, the sample is ready for the next step in 5 minutes..."
Thanks for all of your help!
Cheers, Chris
On Wed, Jan 23, 2019 at 2:30 PM {microwink-at-gmail.com} wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello all, } } I'm working with a student who has metal samples with a ~1um thick } layer of amorphous silicon oxynitride on the surface, and they want to } prepare cross section TEM samples. We have tried gluing the sample } sandwich together with Epo-Tek 353ND (also known as Gatan G1) and } MBond 610, and both stacks fell apart on cutting. } } I assumed that the SiON layers were delaminating, but a visual } inspection using the optical microscope convinced me this was not } likely the underlying cause of the problem. Both epoxies used are not } expired and were cured according to the manufacturer's cure schedule. } I have had no issues with cross sections of other sample systems and } these exact same epoxies, so I wonder if we need to use a different } epoxy chemistry than these standards. Does anyone have experience with } the SiON sample system? Is there a better epoxy to use, like Araldite? } We would like to try to create a sample stack before giving up and } switching to attempting tripod/wedge polishing. } } Thanks for any advice, } Chris } } ==============================Original Headers============================== } 4, 39 -- From microwink-at-gmail.com Wed Jan 23 13:21:53 2019 } 4, 39 -- Received: from mail-wr1-f51.google.com (mail-wr1-f51.google.com [209.85.221.51]) } 4, 39 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x0NJLrhx005869 } 4, 39 -- for {Microscopy-at-microscopy.com} ; Wed, 23 Jan 2019 13:21:53 -0600 } 4, 39 -- Received: by mail-wr1-f51.google.com with SMTP id v13so3858423wrw.5 } 4, 39 -- for {Microscopy-at-microscopy.com} ; Wed, 23 Jan 2019 11:29:03 -0800 (PST) } 4, 39 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 4, 39 -- d=gmail.com; s=20161025; } 4, 39 -- h=mime-version:from:date:message-id:subject:to:cc; } 4, 39 -- bh=973WgAPuCv6b6NA2IaF1iEbj9rGKlO+JTHT92/PfQiI=; } 4, 39 -- b=LRqQUzYEXDPxHuj3MzcyI7uYMiK1lVbI6QeMAu+x0ttPnr23/IP7r/OEju1TKTFqAW } 4, 39 -- g1mKS4gjbvBX28DcsqY0iwVFO3gBvhbV7wlJL8doseWAW8Ejr3aO+eebc7C6mz1ogQbe } 4, 39 -- j9r+s3TZq6Ia0HpEfVs8E+Q+mplUIqmPnGVunMstNWiViLsAIol09WdOYXqpmzO7iu41 } 4, 39 -- jwRb/xw5k6y8GE/Z9uYBnThgyYju4Gvt5iRp1Ntd4dCHXiaXsHwg48KMllyIfu4QecDV } 4, 39 -- jwo7d9P1RzmO4R2TNO8G8aB2r2RT2MvAdUnGLdIqJVpQjF42xCTU3aCUGNhZcRGSIeXv } 4, 39 -- JqPg== } 4, 39 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 4, 39 -- d=1e100.net; s=20161025; } 4, 39 -- h=x-gm-message-state:mime-version:from:date:message-id:subject:to:cc; } 4, 39 -- bh=973WgAPuCv6b6NA2IaF1iEbj9rGKlO+JTHT92/PfQiI=; } 4, 39 -- b=pUGA2r1PE9dqxFjktsw2nRvpCUejTe+7qDgF5u6J0gfNi4MvlXJ1EXttONSfMPOkVG } 4, 39 -- IG1jOXWAwMW+afGhGGwZ5ewsNvdTCBU1rTcPeF6rF68jbftBtF/r/LqnNC4n5newm0q1 } 4, 39 -- H7lCpr8LGscXe+ldCqE/nCriGO0cwj6/khGGsaxu2qhrka5mCZ3G60uUhzEiT83wX04J } 4, 39 -- Dxn1Zc0CEUQpLa5PtLhF0MXP5vJTANtY98z+9lM/zD+tUhgPD6rK8hJIGJDs893YV/a7 } 4, 39 -- areABsts+DuV02WcshohYl4BlWTM1UFGmDyCGOzuqqgQbkmcA0IbVP44rNX0XcTqhAXb } 4, 39 -- j8hQ== } 4, 39 -- X-Gm-Message-State: AJcUukeSpxc+tUYu4PyGj5IUgVJu3jMSQJ6/g3TR5Prpddvq+WuHDzjW } 4, 39 -- ZyV98jmV5m0q2W3K9+A0vQ4oXl3NNDf3OyW49YHc9OHW } 4, 39 -- X-Google-Smtp-Source: ALg8bN7GjXUCJh7Zt3PCB2A4+s2YOKNpqHlf+fT8BvkEfwtu25QmOCUBe9zgVjDZIjQONRY18WQAfas4BZyBghtDnrA= } 4, 39 -- X-Received: by 2002:a5d:550f:: with SMTP id b15mr4235392wrv.330.1548271742333; } 4, 39 -- Wed, 23 Jan 2019 11:29:02 -0800 (PST) } 4, 39 -- MIME-Version: 1.0 } 4, 39 -- From: Christopher Winkler {microwink-at-gmail.com} } 4, 39 -- Date: Wed, 23 Jan 2019 14:28:51 -0500 } 4, 39 -- Message-ID: {CAA8T2PPoF2psxockj2iiLsaYMJOQA6OjG9raJ3kdHDQFPeCSHA-at-mail.gmail.com} } 4, 39 -- Subject: TEM xsect sample preparation - repeated glue failure } 4, 39 -- To: Microscopy-at-microscopy.com } 4, 39 -- Cc: Kaustubh Bawane {kauskb7-at-vt.edu} } 4, 39 -- Content-Type: text/plain; charset="UTF-8" } ==============================End of - Headers==============================
From harremme142vue-at-gmail.com Fri Jan 25 18:00:28 2019 Return-Path: {harremme142vue-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.161.147] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x0Q00R7M006438 for {microscopylistserverarchive7-at-microscopy.com} ; Fri, 25 Jan 2019 18:00:28 -0600 Received: from unknown (HELO mailout.endmonthnow.com) (Fri, 25 Jan 2019 15:59:42 -0800) by mail.webhostings4u.com with QMQP; Fri, 25 Jan 2019 15:59:42 -0800 Received: from unknown (147.182.132.244) by asx121.turbo-inline.com with SMTP; Fri, 25 Jan 2019 15:55:01 -0800 Message-ID: {6B489ADE.7CB4A018-at-gmail.com}
Dear All, may I ask if anyone has any experience is using some 3rd Party makeshift controller to duplicate some functionality of the TEM control panel such as brightness and focus knob? And probably its for JEOL TEM with TEMCenter software.
I come across https://www.3dconnexion.eu/ for CAD software but wonder if one could tweak it for TEM control application.
Any pieces of advice are greatly appreciated!
Cheers, Yee Yan, Tay FACTS, NTU
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I am pleased to announce a new staff position at the Marine Biological Laboratory.Those interested in the position should submit an application, including a CV, a cover letter, and the names and contact information for three references to mbl.edu/RESEA01039
We will begin reviewing applications on February 11, 2019.
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*Imaging Research Specialist/Senior Research Specialist*
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The Marine Biological Laboratory (MBL) invites applications for a research specialist in the MBL’s Microscopy and Imaging Facility. Responsibilities include technical support and training for specimen preparation, image acquisition, and computational processing and analysis using advanced imaging techniques such as light sheet microscopy and scanning electron microscopy array tomography. The successful candidate will receive training on these and other cutting-edge systems from their respective instrument developers, commercial vendors, and resident staff.
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* Support a broad variety of microscopy imaging projects and educational courses * Help implement and refine novel imaging technologies * Provide routine maintenance and troubleshooting on the imaging systems * Provide training in specimen preparation and image processing and analysis * Learn proper specimen preparation and image processing such as segmentation, particle tracking, and shape analysis. * Report to the Director Imaging Services within the Division of Research
Can anybody provide me with some upper limits for the charge density achievable in the focus of a focussed
electron (or ion) beam. The focal point doesn't have to be very small, 100 nm would be easily sufficient.
The speed of the electrons isn't important either.
Another interesting question is the largest possible convergence angle.
Regards.
Philip
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Title-Subject: [Filtered] Employment Opportunity: FIB-SEM Scientist at the Naval Nuclear Laboratory Message: NNL currently has an opening for a highly skilled Focused Ion Beam (FIB) microscopist. At the Naval Nuclear Laboratory (NNL), we develop advanced naval nuclear propulsion technology, ensuring the safety and reliability of our Navy's submarine and aircraft carrier fleets.
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Has anybody ever recorded a transmission image of an electron beam using TEM, STEM or some holographic method?
All the best,
Philip
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What I'm looking for is an actual image of an electron beam. The electron beam should be the specimen and probably orthogonal to the beam used for imaging it. Obviously this requires some sort of dual column setup.
All the best,
Philip
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What's the point? Everhart did this in SEM about 50 years ago. ******************************************************* Chaoying Ni, PhD Director, W. M. Keck Center for Advanced Microscopy and Microanalysis Professor, Materials Science and Engineering http://www.camm.udel.edu; http://www.mseg.udel.edu (302) 831-6359 (Phone); (302) 831-4545 (Fax)
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Mailing address: University of Delaware 201 duPont Hall, Newark, DE 19716 *******************************************************
-----Original Message----- X-from: Philip.Koeck-at-ki.se {Philip.Koeck-at-ki.se} Sent: Friday, February 8, 2019 4:53 AM To: Ni, Chaoying {cni-at-udel.edu}
Hi again.
What I'm looking for is an actual image of an electron beam. The electron beam should be the specimen and probably orthogonal to the beam used for imaging it. Obviously this requires some sort of dual column setup.
All the best,
Philip
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I believe I have an issue with scan rotation calibration. When the image is in focus and the stage is moved, the image does not move in a strait horizontal or vertical direction. The offset is about 3 degrees. I have to under focus by about 10mm to have the image move strait. The "Scan Rotation" button is off and I have never had to use it before. Turing it on and adjusting it to 3 degrees adds some x motion if i am moving y to correct the twist but this isn't precise enough to use stage automation features. Is there a way to adjust the scan rotation calibration on a 5600? -Josh
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I actually mean there should be two beams in the microscope, the electron beam you are imaging with and an additional electron or ion beam that you are taking an image of. Ideally the second beam would be orthogonal to the first. In a TEM this would mean sending in the second beam through the specimen port.
A ronchigram doesn't require two beams, does it?
All the best,
Philip ________________________________________ X-from: John Mardinly [John.Mardinly-at-asu.edu] Sent: 08 February 2019 18:46 To: Philip Köck; MSA
Hi,
do you have a reference to this paper by any chance?
All the best,
Philip
________________________________________ X-from: Ni, Chaoying [cni-at-udel.edu] Sent: 08 February 2019 15:56 To: Philip Köck; Microscopy-at-microscopy.com
Hi again.
What I'm looking for is an actual image of an electron beam. The electron beam should be the specimen and probably orthogonal to the beam used for imaging it. Obviously this requires some sort of dual column setup.
All the best,
Philip
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“To see something” is fundamentally philosophical (remember the Schrodinger’s cat?)
When you use an orthogonal 2nd e-beam or any 2nd beam at any angle to shoot or watch the 1st beam, the resulting “1st“ beam will no longer be the 1st beam when it was not peeked, not to mention if such an experiment would even be possibly reliably realizable in the case of TEM.
In fact, Ronchigram is a good answer, and CBED can be another, I think. By choosing “different amorphous materials or crystals”, i.e. “2nd beams” or “instruments”, together with different focus and beam dose, the beam trajectory information can likely be extracted.
People also use Monte Carlo Methods
The reference is "T. Everhart, P. Hoff, J. Appl. Phys. 42, 5837 (1971)"
Curious at what you actually want to achieve. Can you share?
Good luck! ******************************************************* Chaoying Ni, PhD Director, W. M. Keck Center for Advanced Microscopy and Microanalysis Professor, Department of Materials Science and Engineering http://www.camm.udel.edu; http://www.mseg.udel.edu (302) 831-6359 (Phone); (302) 831-4545 (Fax)
Facility location: 154-174 Harker Laboratory 221 Academy Street, Newark, DE 19716
Mailing address: University of Delaware 201 duPont Hall, Newark, DE 19716 *******************************************************
-----Original Message----- X-from: Philip.Koeck-at-ki.se {Philip.Koeck-at-ki.se} Sent: Saturday, February 9, 2019 3:42 AM To: Ni, Chaoying {cni-at-udel.edu}
Hi,
do you have a reference to this paper by any chance?
All the best,
Philip
________________________________________ X-from: Ni, Chaoying [cni-at-udel.edu] Sent: 08 February 2019 15:56 To: Philip Köck; Microscopy-at-microscopy.com
Hi again.
What I'm looking for is an actual image of an electron beam. The electron beam should be the specimen and probably orthogonal to the beam used for imaging it. Obviously this requires some sort of dual column setup.
All the best,
Philip
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From irapier044ey-at-gmail.com Sat Feb 9 14:21:27 2019 Return-Path: {irapier044ey-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.161.132] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x19KLP9Y031015 for {microscopylistserverarchive7-at-microscopy.com} ; Sat, 9 Feb 2019 14:21:26 -0600 Received: from unknown (HELO mail.naihautsui.co.kr) (Sat, 09 Feb 2019 12:28:24 -0800) by mail.webhostings4u.com with LOCAL; Sat, 09 Feb 2019 12:28:24 -0800 Received: from unknown (138.213.77.177) by smtp.mixedthings.net with ESMTP; Sat, 09 Feb 2019 12:17:48 -0800 Received: from unknown (148.48.106.15) by public.micromail.com.au with QMQP; Sat, 09 Feb 2019 12:03:32 -0800 Message-ID: {4640409F.CFAA8F40-at-gmail.com}
I think it's worth mentioning that this is basically what particle colliders are, except the two beams aren't orthogonal, and people more often use protons--so Phillip isn't crazy to ask. I couldn't quickly find any paper where somebody reconstructed a beam profile based on detected particles, but I'm sure someone has done that. The closest I could quickly find was a paper where someone used braking radiation to image a proton beam: https://doi.org/10.1016/0029-554X(81)90734-5 Not quite what you're looking for, Phillip, but I'm sure it's there. Seems that high-energy physicists are the right people to ask. Tyler
On Sat, Feb 9, 2019 at 2:34 PM cni-at-udel.edu {cni-at-udel.edu} wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } “To see something” is fundamentally philosophical (remember the Schrodinger’s cat?) } } When you use an orthogonal 2nd e-beam or any 2nd beam at any angle to shoot or watch the 1st beam, the resulting “1st“ beam will no longer be the 1st beam when it was not peeked, not to mention if such an experiment would even be possibly reliably realizable in the case of TEM. } } In fact, Ronchigram is a good answer, and CBED can be another, I think. By choosing “different amorphous materials or crystals”, i.e. “2nd beams” or “instruments”, together with different focus and beam dose, the beam trajectory information can likely be extracted. } } People also use Monte Carlo Methods } } The reference is "T. Everhart, P. Hoff, J. Appl. Phys. 42, 5837 (1971)" } } Curious at what you actually want to achieve. Can you share? } } Good luck! } ******************************************************* } Chaoying Ni, PhD } Director, W. M. Keck Center for Advanced Microscopy and Microanalysis } Professor, Department of Materials Science and Engineering } http://www.camm.udel.edu; http://www.mseg.udel.edu } (302) 831-6359 (Phone); (302) 831-4545 (Fax) } } Facility location: } 154-174 Harker Laboratory } 221 Academy Street, Newark, DE 19716 } } Mailing address: } University of Delaware } 201 duPont Hall, Newark, DE 19716 } ******************************************************* } } -----Original Message----- } X-from: Philip.Koeck-at-ki.se {Philip.Koeck-at-ki.se} } Sent: Saturday, February 9, 2019 3:42 AM } To: Ni, Chaoying {cni-at-udel.edu} } Subject: [Microscopy] RE: (electron microscopy) clarification: image of an } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi, } } do you have a reference to this paper by any chance? } } All the best, } } Philip } } ________________________________________ } X-from: Ni, Chaoying [cni-at-udel.edu] } Sent: 08 February 2019 15:56 } To: Philip Köck; Microscopy-at-microscopy.com } Subject: RE: [Microscopy] (electron microscopy) clarification: image of an electron beam } } What's the point? Everhart did this in SEM about 50 years ago. } ******************************************************* } Chaoying Ni, PhD } Director, W. M. Keck Center for Advanced Microscopy and Microanalysis Professor, Materials Science and Engineering http://www.camm.udel.edu; http://www.mseg.udel.edu } (302) 831-6359 (Phone); (302) 831-4545 (Fax) } } Facility location: 154-174 Harker Laboratory } 221 Academy Street, Newark, DE 19716 } } Mailing address: } University of Delaware } 201 duPont Hall, Newark, DE 19716 } ******************************************************* } } -----Original Message----- } X-from: Philip.Koeck-at-ki.se {Philip.Koeck-at-ki.se} } Sent: Friday, February 8, 2019 4:53 AM } To: Ni, Chaoying {cni-at-udel.edu} } Subject: [Microscopy] (electron microscopy) clarification: image of an electron beam } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi again. } } What I'm looking for is an actual image of an electron beam. } The electron beam should be the specimen and probably orthogonal to the beam used for imaging it. } Obviously this requires some sort of dual column setup. } } All the best, } } Philip } } } När du skickar e-post till Karolinska Institutet (KI) innebär detta att KI kommer att behandla dina personuppgifter. Här finns information om hur KI behandlar personuppgifter {https://ki.se/medarbetare/integritetsskyddspolicy} . } } } Sending email to Karolinska Institutet (KI) will result in KI processing your personal data. 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-- Dr. Tyler Harvey Georg-August-Universität Göttingen IV. Physical Institute Friedrich-Hund-Platz 1 37077 Göttingen Germany
Hi Dennis, Harvey, John, Alwyn, Ni, Reinhard and everybody following this.
There seems to be agreement that two beams will intersect each other at right angles seemingly unaffected since the particle density in both is so small.
I'm thinking of the situation with a different model: I treat the "specimen beam" as a charge distribution along a line (roughly speaking). As an example: For a beam of 1keV electrons with about 100 nA focused to about 1 micron beam diameter I get a charge distribution corresponding to about 1 electron every 20 or so microns. This charge distribution along a line produces an electrostatic potential which I approximate as smooth (on average). The electron beam used for imaging this specimen, on the other hand, I describe as a monochromatic, plane wave. This wave should be phase shifted as it travels through the potential. The phase shift is proportional to the projected potential. The relative phase shift I get for 200 keV electrons is in the range of pi, but it's a very slowly varying spatial distribution. To see this as an image would require a true phase contrast technique that has a good contrast transfer at very low spatial frequencies. Maybe off-axis holography would work. This fits very well with the observation that two beams visibly hardly influence each other. Most scattering is restricted to very small angles, since the phase shift varies very slowly. (I know I'm jumping around between the wave and particle picture.)
Just to clarify: I'm not planning to do this experiment. I have something else in mind.
About the specimen beam current I mention (100 nA): It seems high, I guess, but I've been told this is easily achievable in e-beam lithography systems. I haven't found a paper confirming this, though.
Here's another interesting paper I got via a colleague on Research Gate: https://arxiv.org/ftp/arxiv/papers/1610/1610.05602.pdf
I think it's worth mentioning that this is basically what particle colliders are, except the two beams aren't orthogonal, and people more often use protons--so Phillip isn't crazy to ask. I couldn't quickly find any paper where somebody reconstructed a beam profile based on detected particles, but I'm sure someone has done that. The closest I could quickly find was a paper where someone used braking radiation to image a proton beam: https://doi.org/10.1016/0029-554X(81)90734-5 Not quite what you're looking for, Phillip, but I'm sure it's there. Seems that high-energy physicists are the right people to ask. Tyler
On Sat, Feb 9, 2019 at 2:34 PM cni-at-udel.edu {cni-at-udel.edu} wrote: } } “To see something” is fundamentally philosophical (remember the Schrodinger’s cat?) } } When you use an orthogonal 2nd e-beam or any 2nd beam at any angle to shoot or watch the 1st beam, the resulting “1st“ beam will no longer be the 1st beam when it was not peeked, not to mention if such an experiment would even be possibly reliably realizable in the case of TEM. } } In fact, Ronchigram is a good answer, and CBED can be another, I think. By choosing “different amorphous materials or crystals”, i.e. “2nd beams” or “instruments”, together with different focus and beam dose, the beam trajectory information can likely be extracted. } } People also use Monte Carlo Methods } } The reference is "T. Everhart, P. Hoff, J. Appl. Phys. 42, 5837 (1971)" } } Curious at what you actually want to achieve. Can you share? } } Good luck! } ******************************************************* } Chaoying Ni, PhD } ******************************************************* } } -----Original Message----- } ---------------------------------------------------------------------------- } } Hi again. } } What I'm looking for is an actual image of an electron beam. } The electron beam should be the specimen and probably orthogonal to the beam used for imaging it. } Obviously this requires some sort of dual column setup. } } All the best, } } Philip
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A lot of interest is beginning (here at UNMC) in the morphology of mitochondria under normal and experimental conditions. Now we all know that it is easy to get artifacts in mitochondria during fixation. Some of the questions from our various researchers involve looking for what I would consider slight or subtle changes. Things that any artifacts would complicate the interpretation of in the images.
So, I'm asking, does anyone out there have a fixative that will give preserved, artifact free mitochondria? If you have a favorite recipe, I would like you to share it with me. I should point out that the most likely type of fixation will be immersion fixation not perfusion.
Bonus question, can anyone explain what I would call the current fad, this interest in morphological changes in mitochondria? I'm sure we are not the only EM Lab getting these questions about mitochondria which seems to be an up and coming topic in medical research. All help is appreciated. Thank you.
Tom Bargar UNMC Electron Microscopy Core Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
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From ronaldfaust205xd-at-gmail.com Wed Feb 13 01:26:42 2019 Return-Path: {ronaldfaust205xd-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.161.131] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x1D7Qffi023626 for {microscopylistserverarchive7-at-microscopy.com} ; Wed, 13 Feb 2019 01:26:41 -0600 Received: from unknown (164.63.30.235) by relay37.vosimerkam.net with NNFMP; Wed, 13 Feb 2019 01:16:13 -0600 Received: from smtp18.yenddx.com ([Wed, 13 Feb 2019 01:08:38 -0600]) by asx121.turbo-inline.com with ESMTP; Wed, 13 Feb 2019 01:08:38 -0600 Message-ID: {0888917C.2F714F84-at-gmail.com}
Dear Listers,
Could you, please, point me to the good and reliable source of secondary antibodies conjugated to colloidal gold (5-10-15nm) in UK or Europe?
Previously, we used BBI a lot but it seems that now they have very limited variety.
Thank you in advance!
Sincerely,
Alex
-- Dr. Aleksandr Mironov MD, PhD Senior Experimental Officer D.1527, M.Smith Building EM Core Facility School of Biological Sciences, Faculty of Biology Medicine and Health University of Manchester Oxford Road Manchester M13 9PT UK
==============================Original Headers============================== 9, 31 -- From Aleksandr.Mironov-at-manchester.ac.uk Wed Feb 13 04:46:37 2019 9, 31 -- Received: from clarity.mcc.ac.uk (clarity.mcc.ac.uk [130.88.200.144]) 9, 31 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x1DAkaei000527 9, 31 -- for {Microscopy-at-microscopy.com} ; Wed, 13 Feb 2019 04:46:37 -0600 9, 31 -- Received: from asmtp2.its.manchester.ac.uk ([130.88.13.150]:52530) 9, 31 -- by clarity.mcc.ac.uk with esmtps (TLSv1.2:ECDHE-RSA-AES256-GCM-SHA384:256) 9, 31 -- (Exim 4.91 (FreeBSD)) 9, 31 -- (envelope-from {Aleksandr.Mironov-at-manchester.ac.uk} ) 9, 31 -- id 1gtsBp-0000vj-Ru 9, 31 -- for Microscopy-at-microscopy.com; Wed, 13 Feb 2019 10:54:53 +0000 9, 31 -- Received: from [10.242.61.228] (port=52767) 9, 31 -- by asmtp2.its.manchester.ac.uk with esmtpsa (TLSv1.2:ECDHE-RSA-AES128-GCM-SHA256:128) 9, 31 -- (Exim 4.91) 9, 31 -- (envelope-from {Aleksandr.Mironov-at-manchester.ac.uk} ) 9, 31 -- id 1gtsBp-0002bD-Q8 9, 31 -- for Microscopy-at-microscopy.com; Wed, 13 Feb 2019 10:54:53 +0000 9, 31 -- To: Microscopy-at-microscopy.com 9, 31 -- From: Aleksandr Mironov {Aleksandr.Mironov-at-manchester.ac.uk} 9, 31 -- Subject: TEM: secondary gold conjugates 9, 31 -- Message-ID: {bbcddcf6-d173-1ddb-d27f-26313b6424d3-at-manchester.ac.uk} 9, 31 -- Date: Wed, 13 Feb 2019 10:54:53 +0000 9, 31 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:52.0) Gecko/20100101 9, 31 -- Thunderbird/52.9.1 9, 31 -- MIME-Version: 1.0 9, 31 -- Content-Type: text/plain; charset=utf-8; format=flowed 9, 31 -- Content-Transfer-Encoding: 7bit 9, 31 -- Content-Language: en-US 9, 31 -- X-Authenticated-Sender: Aleksandr Mironov from ([10.242.61.228]) [10.242.61.228]:52767 9, 31 -- X-Authenticated-From: Aleksandr.Mironov-at-manchester.ac.uk 9, 31 -- X-Spam-Score: -5.0(?) 9, 31 -- X-Spam-Flag: NO ==============================End of - Headers==============================
From ketcdutj170si-at-gmail.com Wed Feb 13 13:18:47 2019 Return-Path: {ketcdutj170si-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.161.146] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x1DJIjDq032262 for {microscopylistserverarchive7-at-microscopy.com} ; Wed, 13 Feb 2019 13:18:47 -0600 Received: from unknown (212.84.232.52) by public.micromail.com.au with QMQP; Wed, 13 Feb 2019 08:11:03 -1100 Message-ID: {54D070FE.9D3FF798-at-gmail.com}
Hi everyone.
we have a strange result in an FFT of a hi res stem image.
We have a series of vertical lines that seem to be noise from somewhere. I can send the original image and/or the FFT if someone would be kind enough to offer to take a look!
Thanks!
​​​​​ Chris
Christopher J. Gilpin Ph.D. Campus-wide Coordinator for Electron Microscopy Director, Life Science Microscopy Facility Purdue University Whistler Hall of Agriculture Research, Room S052 170 S. University St West Lafayette, IN 47907 765-494-7750 gilpin-at-purdue.edu lsmf-at-purdue.edu reaches everyone in the facility. http://ag.purdue.edu/arp/Microscopy/Pages/default.aspx skype cjgilpin
==============================Original Headers============================== 10, 32 -- From gilpin-at-purdue.edu Wed Feb 13 17:20:46 2019 10, 32 -- Received: from xppmailspam02.itap.purdue.edu (xppmailspam02.itap.purdue.edu [128.210.5.13]) 10, 32 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x1DNKjuU013543 10, 32 -- for {microscopy-at-microscopy.com} ; Wed, 13 Feb 2019 17:20:46 -0600 10, 32 -- X-IronPort-Anti-Spam-Filtered: true 10, 32 -- X-IronPort-AV: E=Sophos;i="5.58,366,1544504400"; 10, 32 -- d="scan'208";a="214514972" 10, 32 -- Received: from exchange.purdue.edu ([128.210.1.29]) 10, 32 -- by xppmailspam02.itap.purdue.edu with ESMTP/TLS/AES256-SHA; 13 Feb 2019 18:29:05 -0500 10, 32 -- Received: from wppexc07.purdue.lcl (172.30.136.180) by WPPEXC16.purdue.lcl 10, 32 -- (172.30.136.189) with Microsoft SMTP Server (TLS) id 15.0.1367.3; Wed, 13 Feb 10, 32 -- 2019 18:29:04 -0500 10, 32 -- Received: from wppexc07.purdue.lcl ([fe80::49db:3fa0:d668:8da4]) by 10, 32 -- wppexc07.purdue.lcl ([fe80::49db:3fa0:d668:8da4%14]) with mapi id 10, 32 -- 15.00.1365.000; Wed, 13 Feb 2019 18:29:04 -0500 10, 32 -- From: "Gilpin, Christopher J" {gilpin-at-purdue.edu} 10, 32 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 10, 32 -- Subject: help with stem FFT interpretation 10, 32 -- Thread-Topic: help with stem FFT interpretation 10, 32 -- Thread-Index: AdTD8z3ca5gi4GgeTMGi/8Ku4QZsjA== 10, 32 -- Date: Wed, 13 Feb 2019 23:29:04 +0000 10, 32 -- Message-ID: {69003d5ab7ed4588b2662f8014ad90d4-at-wppexc07.purdue.lcl} 10, 32 -- Accept-Language: en-US 10, 32 -- Content-Language: en-US 10, 32 -- X-MS-Has-Attach: 10, 32 -- X-MS-TNEF-Correlator: 10, 32 -- x-ms-exchange-transport-fromentityheader: Hosted 10, 32 -- x-originating-ip: [10.163.23.200] 10, 32 -- Content-Type: text/plain; charset="utf-8" 10, 32 -- MIME-Version: 1.0 10, 32 -- Content-Transfer-Encoding: 8bit 10, 32 -- X-MIME-Autoconverted: from base64 to 8bit by microscopy.com id x1DNKjuU013543 ==============================End of - Headers==============================
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Email: dlowry-at-asu.edu Name: David Lowry
Organization: Arizona State University
Title-Subject: [Filtered] negative staining and protein chemistry question
Message: A grad student working in our lab has encountered a problem involving TEM negative staining of 2 different protein molecules. The proteins are purified commercial products and are clearly distinct from one another. In her experiments, the 2 proteins stain well (uranyl acetate) and are easily resolved when they are applied individually to glow-discharged grids. However, in experiments when the 2 proteins are pre-mixed, one of them acquires stain and is resolved normally, but the other one cannot be resolved, it either does not stain correctly or does not bind to the grid surface. The buffer used for all of her trials is the same (5 mM HEPES, pH 7.2). We are looking for possible explanations for why this may be occurring from list users who are knowledgeable of protein chemistry thank you- Login Host: 149.169.80.88 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
Hi all, Does anyone have the schematics for a Ted Pella vibratome 3000? If yes, can you send them to me? The vibratome is not advancing or retreating normally. It does advance and retreat but super slowly and the normal noises it makes aren't happening. If you can troubleshoot this problem with me I would greatly appreciate it! Ted Pella will not service it nor will Leica (who bought out Vibratome) and the quote from Southeast Pathology Services is more than we can afford at this time. With your help I am ready to dissect this machine. SPS suggested that the barrel might be stuck. The foot pedal is not attached. Have a Happy Valentine's Day, Beth at Georgia Electron Microscopy, UGA, Athens, GA
We've recently hosted a Plunge freezing Leica GP2, and I'm getting used of it. We've got very high viscosity samples to do CryoTEM and I'm wondering whether multiple blots would work better then single blot.
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From gunnclar77uwnf-at-gmail.com Sun Feb 17 02:34:50 2019 Return-Path: {gunnclar77uwnf-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.161.133] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x1H8YmTn021927 for {microscopylistserverarchive7-at-microscopy.com} ; Sun, 17 Feb 2019 02:34:49 -0600 Received: from unknown (212.140.130.71) by qrx.quickslick.com with ESMTP; Sun, 17 Feb 2019 18:26:17 +1000 Received: from asx121.turbo-inline.com [129.54.236.249] by mail.naihautsui.co.kr with ASMTP; Sun, 17 Feb 2019 18:08:50 +1000 Received: from webmail.halftomorrow.com ([88.133.212.187]) by smtp4.cyberemailings.com with NNFMP; Sun, 17 Feb 2019 18:08:41 +1000 Message-ID: {11ECA1D4.0DA19F2F-at-gmail.com}
We have a project working on cyanobacteria and have discovered a need to do freeze-etch EM. We don't have the equipment to do this, so we are looking for a lab that can do freeze-etch on a contract basis.
Phil ------------- Philip Oshel Imaging Facility Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 989 774-3576 office 989 774-7567 lab
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Title-Subject: [Filtered] microwaving large samples in osmium
Message: Hello. Does anyone have any experience with microwaving large samples( 1.2 mm thick or larger) in osmium? I am wondering about time and wattage to increase the penetration of osmium. So far I have only tried 6 min at 400 w. thanks in advance JoAnn
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Title-Subject: [Filtered] Tips for TEM SAED JEOL 2100F
Message: Hello,
I would like to ask some practical tips on performing SAED on a JEOL 2100F TEM. I am a complete newbie to TEM diffraction and the “2100 user manual” diffraction chapter is rather limited in describing influence of various settings (or recommendation of it) on the outcome of the diffraction patterns i.e. use of condenser lenses, alpha, spot values, required/suggested alignment/correction procedures. I usually (1)focus on the sample in question at about 100kx, (2) perform voltage centering alignment (HT WOB), (3) WOB Y/X correction. And then switch to the SAED procedure in chapter 5.5.1 of the manual.
My samples are micron sized fly ash particles (silicates, aluminosilicates). Fixed on holey carbon films on copper mesh. The TEM is operated at 200kV. So far one of the most notable “problems” I have is: (1)After selecting/centering the area of interest on the fluorescent screen (SA MAG mode, field limiting aperture inserted) I switch to SA DIFF mode and the beam shifts position to the top left (midway radius) on the fluorescent screen which makes navigation on the sample very difficult. (2)Using the DIFF FOCUS knob to focus the beam into a small spot, the diffraction patterns do not appear completely spherical (mild ellipse).
Any practical tips/steps on setting up the scope for the best SAED experience would be greatly appreciated.
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You are performing the basic procedures involved in selected area diffraction just fine. Regarding the issues you are having, (1) I'm not sure what you mean by the beam shifting when you switch to diffraction mode. Is the area you are selecting in MAG mode physically shifting outside the aperture (if so, you can adjust the image shifts to correct this), or is the under/overfocused image simply shifting on the green screen? To correct the later, turn on your projector shifts (PLA on JEOL) and adjust the image back to the center of the green screen.
(2)To correct astigmatism in the intermediate lenses, you'll need to use your intermediate lens stigmator. On the JEOL, this is only accessible through the alignment panel in the TEMCON software. You can obtain the caustic spot in diffraction mode and correct using that, or use an amorphous area of your sample.
Contact me directly if you need more detailed procedures.
Good luck, Chris
On Mon, Feb 18, 2019 at 8:19 PM {microscopy.listserver-at-gmail.com} wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } } } -------- Forwarded Message -------- } } } X-from: arunas.mesceriakovas-at-uef.fi } } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy to both arunas.mesceriakovas-at-uef.fi, } Microscopy-at-Microscopy.com so that all Microscopy Listserver } Subscribers can benefit from our collective wisdom } --------------------------------------------------------------------------- } } Email: arunas.mesceriakovas-at-uef.fi Name: Arunas } } Title-Subject: [Filtered] Tips for TEM SAED JEOL 2100F } } Message: Hello, } } I would like to ask some practical tips on performing SAED on a JEOL } 2100F TEM. I am a complete newbie to TEM diffraction and the “2100 user } manual” diffraction chapter is rather limited in describing influence of } various settings (or recommendation of it) on the outcome of the } diffraction patterns i.e. use of condenser lenses, alpha, spot values, } required/suggested alignment/correction procedures. } I usually (1)focus on the sample in question at about 100kx, (2) perform } voltage centering alignment (HT WOB), (3) WOB Y/X correction. And then } switch to the SAED procedure in chapter 5.5.1 of the manual. } } My samples are micron sized fly ash particles (silicates, } aluminosilicates). Fixed on holey carbon films on copper mesh. The TEM } is operated at 200kV. So far one of the most notable “problems” I have is: } (1)After selecting/centering the area of interest on the fluorescent } screen (SA MAG mode, field limiting aperture inserted) I switch to SA } DIFF mode and the beam shifts position to the top left (midway radius) } on the fluorescent screen which makes navigation on the sample very } difficult. (2)Using the DIFF FOCUS knob to focus the beam into a small } spot, the diffraction patterns do not appear completely spherical (mild } ellipse). } } } Any practical tips/steps on setting up the scope for the best SAED } experience would be greatly appreciated. } } Login Host: 193.167.228.180 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 18, 53 -- From microscopy.listserver-at-gmail.com Mon Feb 18 19:09:33 2019 } 18, 53 -- Received: from mail-pf1-f176.google.com (mail-pf1-f176.google.com [209.85.210.176]) } 18, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x1J19WD7024698 } 18, 53 -- for {microscopy-at-microscopy.com} ; Mon, 18 Feb 2019 19:09:33 -0600 } 18, 53 -- Received: by mail-pf1-f176.google.com with SMTP id q17so9376234pfh.10 } 18, 53 -- for {microscopy-at-microscopy.com} ; Mon, 18 Feb 2019 17:18:09 -0800 (PST) } 18, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 18, 53 -- d=gmail.com; s=20161025; } 18, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 18, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 18, 53 -- bh=hxHJEHumJGbYROGT2C4m52y50N+kw6/bzQ7hh2LKYEs=; } 18, 53 -- b=p+BjzHlDgkahm7ixcWcgR8x6Euzo/P7FtR587nVNf5RlyrHqgQnqPqzoL8Slkg4yD8 } 18, 53 -- gVrCoOmiCqWGNpEfhHGF+2mI/7TbSfr20pvOdQNFGOSPuoYAgX7wKjMT3+u8k8sV5PL7 } 18, 53 -- XLpGTlPY4R/u/7CScKnQFPqfas98KPO+th1yaiuQya3N3x4JtPqyz5njK8LCHHNecYq2 } 18, 53 -- hYPqgfshgJeo+SGszQZ4PnsHpaK0qP5YVpWNIT1B48BoxD+dpFpiXzVZXdz6FSf5P/XV } 18, 53 -- I59QkQOvjLq1IUKFfO5EwwPu7RbRWfO0g90IHfQm9RUZrgvHsl9DrFUScGVqEXbEfW7f } 18, 53 -- DFkA== } 18, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 18, 53 -- d=1e100.net; s=20161025; } 18, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 18, 53 -- :user-agent:mime-version:in-reply-to:content-language } 18, 53 -- :content-transfer-encoding; } 18, 53 -- bh=hxHJEHumJGbYROGT2C4m52y50N+kw6/bzQ7hh2LKYEs=; } 18, 53 -- b=P7UVp/EvB+sfNIRhY7Bf7mmlN+xeb7s38loKT0yYlbyS5Ign62+5zoht779Le9e3oP } 18, 53 -- 7Wuk2X5aLUK+rtr1ZJXRDGVWvke8GRXANVQ2GJ3ofLBf3fdTq8GBsKKHyjGkA6x6szBe } 18, 53 -- MrsvSagcf2Mx2r5eoDQc21oLmzNj2s2JrEZTqbjieaNcjMIscAMFgmNTTW8X2aYAT/S9 } 18, 53 -- x7eeGQ/mjfivlDPljZaL/dHed7JigeR24rjMEPmb9yz2DC+iqsLoYGSOkEcsa7+3NUZb } 18, 53 -- pdH9+iS5wkHKre0I6bN7XADCVbyy8lU6wth9rtLksQZxLFNWxxVOMnYi1wHU+ntKPUjd } 18, 53 -- qKPg== } 18, 53 -- X-Gm-Message-State: AHQUAuYMAdRYMy+8u9aiTCSMNKN/Eod6HHgVQjX3HNn3D9ZaEdgKxpwQ } 18, 53 -- MCcNwGt8VgRtNMnuINVlpnOIX2GX } 18, 53 -- X-Google-Smtp-Source: AHgI3IbNBr3fRu5TTVBhIpdDe6XpKDIEgVUgjdm0Q7+BH3j6Dya+8FG5gDr/fnwDMeqEFH4bciHG7g== } 18, 53 -- X-Received: by 2002:a62:be0c:: with SMTP id l12mr26337801pff.51.1550539088539; } 18, 53 -- Mon, 18 Feb 2019 17:18:08 -0800 (PST) } 18, 53 -- Received: from vlan-2696-10-17-143-100.guest.wireless.sydney.edu.au (natp-s01-129-78-56-212.gw.usyd.edu.au. [129.78.56.212]) } 18, 53 -- by smtp.googlemail.com with ESMTPSA id y9sm22084454pfi.74.2019.02.18.17.18.07 } 18, 53 -- for {microscopy-at-microscopy.com} } 18, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 18, 53 -- Mon, 18 Feb 2019 17:18:07 -0800 (PST) } 18, 53 -- Subject: viaWWW: Tips for TEM SAED JEOL 2100F } 18, 53 -- References: {201902180855.x1I8tJvo007434-at-microscopy.com} } 18, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 18, 53 -- From: Microscopy Listserver {microscopy.listserver-at-gmail.com} } 18, 53 -- X-Forwarded-Message-Id: {201902180855.x1I8tJvo007434-at-microscopy.com} } 18, 53 -- Message-ID: {fba93a9a-2088-8d44-e6ad-fa51d8745f3d-at-gmail.com} } 18, 53 -- Date: Tue, 19 Feb 2019 12:18:05 +1100 } 18, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.13; rv:60.0) } 18, 53 -- Gecko/20100101 Thunderbird/60.5.0 } 18, 53 -- MIME-Version: 1.0 } 18, 53 -- In-Reply-To: {201902180855.x1I8tJvo007434-at-microscopy.com} } 18, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 18, 53 -- Content-Language: en-US } 18, 53 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers==============================
Dear JoAnn, it would be interesting to get info about the type of MW-apparatus (brand) you are using....and how.....(water-load, intermittent on-off, etc.-, etc.). Could you - please - tell "us" any experiences regarding ultrastructural tissue preservation or obvious alterations due to your MW-procedere?
Unfortunately -in former times - I only sometimes used MW for (RAPID) osmication of specimens (up to 4-6 mmł) - .... always with a waterload to prevent boiling of the OsO4-solution (I promise: not only a really great mess if 'exploding' out from the vial but also hazardous-especially to health...!) and just intermittently irradiated for say 5-10x 1-2 seconds (rotary table/disk in action, full power)
- when I tried to RAPIDLY process for diagnostic purposes...(but always processed specimens also in parallel as usual by immersion into 1% buffered OsO4-solution + permanent rotation of vials).
These times of practical experience or "doing osmication myself" were a long time ago....
so:
(Full bibliographic info and abstracts in part added only for convenience of reading....or choice...../.
you might read: https://www.ncbi.nlm.nih.gov/pubmed/7730590 = https://journals.sagepub.com/doi/pdf/10.1177/43.5.7730590 J Histochem Cytochem. 1995 May;43(5):515-23. Rapid primary microwave-aldehyde and microwave-osmium fixation: improved detection of rat parotid acinar cell secretory granule alpha-amylase using a post-embedding immunogold ultrastructural morphometric analysis. Login GR1, Ku TC, Dvorak AM. Author information Abstract Studies of methods for improved fixation are becoming increasingly important in the field of quantitative immunocytochemistry. We used microwave (MW)-assisted chemical fixation to show improved retention of salivary gland acinar cell secretory granule alpha-amylase detected by a quantitative immunogold method. Blocks (4-mmł) of rat parotid gland were fixed by the following methods: (a) MW irradiation in an aldehyde fixative (AF) for 6 sec; (b) immersion in AF for 1.5 hr; (c) MW irradiation in osmium tetroxide (OT) for 9 sec; (d) immersion in OT for 1.5 hr; or (e) Sequential MW AF, 10 sec, MW OT rapid treatment (SMAORT), 10 sec. Specimens were processed routinely for transmission electron microscopy. Thin sections of Epon-embedded tissues were exposed first to rabbit IgG anti-human salivary alpha-amylase and second to gold-conjugated goat anti-rabbit IgG. Granule area was obtained by a point counting method. Labeling density was calculated as the number of gold particles/micron 2 +/- SD. Specimens fixed in seconds by MW-AF, MW-OT, or SMAORT showed ultrastructural preservation similar to immersion fixation in AF or OT for 1.5 hr. Immunogold labeling density of granule alpha-amylase was highest for SMAORT (874 microns 2) compared to MW-AF (295 microns 2), MW-OT (248 microns 2), routine sequential immersion in AF and OT (229 microns 2), or immersion in OT (no aldehyde) (190 microns 2). This study establishes the improved retention of salivary gland acinar cell secretory granule alpha-amylase and markedly enhanced fixation speed for ultrastructural studies made possible by MW-chemical fixation protocols that use aldehydes and osmium. https://journals.sagepub.com/doi/pdf/10.1177/43.5.7730590
www.uic.umn.edu/sites/uic.umn.edu/files/mw_em_protocol_mas.pdf = http://uic.umn.edu/sites/uic.umn.edu/files/mw_em_protocol_mas.pdf Microwave Processing Protocol for Electron Microscopyusing the PELCO BioWave®Laboratory Tissue Processing System Getting Started ... ... Osmium Tetroxide Fixation (see Fig. 1 for set-up) Osmium Wattage Time Vacuum 80W 2min. on - 2 min. off - 2min. on Yes Cool to 20°C and Repeat Time SequenceNotes: 1. Osmium is best used in concentrations of 1% or less. Aqueous works well and is our choice. 2. Reduced osmium works well also (3% potassium ferricyanide mixed with an equal volume of 2% osmium justbefore use). 3. Higher concentrations of osmium will retard penetration of fixative into tissues in a microwave environment
FRMS, Retired Member of MSA Scientific Profile at ResearchGate: http://www.researchgate.net/profile/Wolfgang_MUSS Former Head of Electron Microscopy Lab at Institute of Pathology SALK-LKH / Salzburger Landeskliniken | General Hospital and PMU (private) PARACELSUS MEDICAL UNIVERSITY of SALZBURG
Former Secretary and (until June2017) Board Member of the
SCUR {The Society for Cutaneous Ultrastructure Research} „The Skin Imaging Society“ { www.scur.org }
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Title-Subject: [Filtered] microwaving large samples in osmium
Message: Hello. Does anyone have any experience with microwaving large samples( 1.2 mm thick or larger) in osmium? I am wondering about time and wattage to increase the penetration of osmium. So far I have only tried 6 min at 400 w. thanks in advance JoAnn
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From janiceking866i-at-gmail.com Mon Feb 18 23:11:00 2019 Return-Path: {janiceking866i-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.161.144] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x1J5AxPg025253 for {microscopylistserverarchive7-at-microscopy.com} ; Mon, 18 Feb 2019 23:11:00 -0600 Received: from rly04.hottestmile.com ([126.223.98.167]) by smtp-server1.cfdenselr.com with SMTP; Tue, 19 Feb 2019 01:01:03 -0400 Message-ID: {BE130275.822293F5-at-gmail.com}
Arunas, If your TEM is on service contract request a service visit and ask the engineer to demonstrate on a standard sample how they qualify that the TEM is functioning properly.
It is important that the beam and gun shifts and tilts are the same in imaging and diffraction modes (so the beam is where you think). This should be visible on a diagnostic page. On JEOL 2010 and older scopes it was pages 4 and 5 on the display. If alignments were done in "engineer mode" properly this should all be good. (I've been around 3 decades and have seen strange things; mistakes happen, people get distracted.)
In general I agree with the advice that Chris already provided.
Title-Subject: [Filtered] Tips for TEM SAED JEOL 2100F
Message: Hello,
I would like to ask some practical tips on performing SAED on a JEOL 2100F TEM. I am a complete newbie to TEM diffraction and the "2100 user manual" diffraction chapter is rather limited in describing influence of various settings (or recommendation of it) on the outcome of the diffraction patterns i.e. use of condenser lenses, alpha, spot values, required/suggested alignment/correction procedures. I usually (1)focus on the sample in question at about 100kx, (2) perform voltage centering alignment (HT WOB), (3) WOB Y/X correction. And then switch to the SAED procedure in chapter 5.5.1 of the manual.
My samples are micron sized fly ash particles (silicates, aluminosilicates). Fixed on holey carbon films on copper mesh. The TEM is operated at 200kV. So far one of the most notable "problems" I have is: (1)After selecting/centering the area of interest on the fluorescent screen (SA MAG mode, field limiting aperture inserted) I switch to SA DIFF mode and the beam shifts position to the top left (midway radius) on the fluorescent screen which makes navigation on the sample very difficult. (2)Using the DIFF FOCUS knob to focus the beam into a small spot, the diffraction patterns do not appear completely spherical (mild ellipse).
Any practical tips/steps on setting up the scope for the best SAED experience would be greatly appreciated.
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YES, I have been having trouble with resharpened knives from Diatome!
I sent three knives to be resharpened in July of 2018 (resharpen 2, get one free). I received the first one back and a coworker began using it immediately. When I received the other two back, I found several knife marks on both knives and returned them both. I received a "new" knife in return, which I also found had knife marks. This was returned, and another one was sent. There were a few very faint marks on this one as well, but I was desperate and kept it. It seems to me that it is much more prone to damage from normal use than any knife I have had before, and I am very disappointed in it as well.
Thanks for the vent! I think I will try Microstar again.
Sharon
-----Original Message----- X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Wednesday, February 20, 2019 6:18 PM To: Matthews,Sharon W {Sharon.Matthews-at-medicine.ufl.edu}
Oh My Dear Claudia,
Don't get me going on this subject. I am so disappointed in Diatome, a company I have been dealing with for 40+ years. The quality of their knives has declined over the years and I don't know why - maybe it's because they think they're the only game in town? I swapped an old knife and a considerable sum of money for a new one. There wasn't a single mm without scratches or knife marks on this "new" knife, and all sections showed chatter. I sent the knife back to them and, in their defense, they had another knife on my desk the following day. This knife seemed to be useful on the far left, but when I got to the middle of the blade, same stuff started happening - scratches and knife marks. I move to the far right and it was OK, but it looks like I'm stuck with a 3/4 of a knife. I don't have time to go 'round and 'round with Diatome. Diatome used to be a great company, producing very high quality knives that were great across the entire edge. Not so much anymore. Tried Pella knives - they're even worse. Tried DDK - useless. Microstar is the only one left. I had a loaner knife when I first took this job and the only thing I didn't like about the Microstar knives was that the boat seemed really hydrophobic; other than that, the edge was darned good. BTW, you should be hearing from my friend Lita, who works across the street at the Hughes Institute. She and I have been disappointed in Diatome for several years.
Best to you, Debra Townley Baylor College of Medicine
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Wednesday, February 20, 2019 5:17 PM To: Townley, Debra
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Claudia Same trouble ! (France) We received a « resharpened one" with many marks at the first use (on animal tissues embedded in Epoxy resin) I sent them TEM images of the marks! They told me that it was impossible that a such damaged diamant knife escaped the vigilance of the quality control of the Diatome factory! We did not succeed to have a new one! Best regards Jeannine ________________________________________ De : debrat-at-bcm.edu {debrat-at-bcm.edu} Envoyé : jeudi 21 février 2019 16:11 Ŕ : Jeannine Lherminier Objet : [Microscopy] RE: viaWWW:Resharpened knife quality
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Oh My Dear Claudia,
Don't get me going on this subject. I am so disappointed in Diatome, a company I have been dealing with for 40+ years. The quality of their knives has declined over the years and I don't know why - maybe it's because they think they're the only game in town? I swapped an old knife and a considerable sum of money for a new one. There wasn't a single mm without scratches or knife marks on this "new" knife, and all sections showed chatter. I sent the knife back to them and, in their defense, they had another knife on my desk the following day. This knife seemed to be useful on the far left, but when I got to the middle of the blade, same stuff started happening - scratches and knife marks. I move to the far right and it was OK, but it looks like I'm stuck with a 3/4 of a knife. I don't have time to go 'round and 'round with Diatome. Diatome used to be a great company, producing very high quality knives that were great across the entire edge. Not so much anymore. Tried Pella knives - they're even worse. Tried DDK - useless. Microstar is the only one left. I had a loaner knife when I first took this job and the only thing I didn't like about the Microstar knives was that the boat seemed really hydrophobic; other than that, the edge was darned good. BTW, you should be hearing from my friend Lita, who works across the street at the Hughes Institute. She and I have been disappointed in Diatome for several years.
Best to you, Debra Townley Baylor College of Medicine
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Wednesday, February 20, 2019 5:17 PM To: Townley, Debra
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==============================Original Headers============================== 27, 41 -- From jeannine.lherminier-at-inra.fr Thu Feb 21 10:38:05 2019 27, 41 -- Received: from smtp-edge.dcidf.inra.fr (smtp-edge.dcidf.inra.fr [138.102.156.154]) 27, 41 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x1LGc4fG027255 27, 41 -- for {Microscopy-at-Microscopy.com} ; Thu, 21 Feb 2019 10:38:05 -0600 27, 41 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 27, 41 -- s=s1024; d=inra.fr; 27, 41 -- h=from:to:cc:subject:date:message-id:in-reply-to:content-type: 27, 41 -- mime-version; 27, 41 -- bh=/62qbr4suoNkHIenUR1WrLxm36whCi6naBI+jEnNDpA=; 27, 41 -- b=YwZYsGLq7tkhAeQBl1iC5GzwPJ5bCKVdg1L3GG+PSMciJ1VIdJMBP/jGZYT+OC 27, 41 -- SlpD7DH9lB3jDtqZ2hIqIyz4vBngSCa7TPzbC2L8v62hbqGlqd709G4eVU7t7e 27, 41 -- kqfxb9KwrlOJu/kAobOQiIqYFbMvbyKVyL3ZMh9XHRUin3k= 27, 41 -- Received: from idfdcpripexmu03.inra.local (138.102.152.153) by 27, 41 -- IDFDCPUBEXEDG01.inra.local (138.102.156.154) with Microsoft SMTP Server (TLS) 27, 41 -- id 15.0.1395.4; Thu, 21 Feb 2019 17:46:48 +0100 27, 41 -- Received: from idfdcpripexmu03.inra.local (138.102.152.153) by 27, 41 -- idfdcpripexmu03.inra.local (138.102.152.153) with Microsoft SMTP Server (TLS) 27, 41 -- id 15.0.1395.4; Thu, 21 Feb 2019 17:46:48 +0100 27, 41 -- Received: from idfdcpripexmu03.inra.local ([fe80::7964:d04c:1360:fb50]) by 27, 41 -- idfdcpripexmu03.inra.local ([fe80::7964:d04c:1360:fb50%22]) with mapi id 27, 41 -- 15.00.1395.000; Thu, 21 Feb 2019 17:46:48 +0100 27, 41 -- From: Jeannine Lherminier {jeannine.lherminier-at-inra.fr} 27, 41 -- To: "lopezcl-at-ohsu.edu" {lopezcl-at-ohsu.edu} 27, 41 -- CC: "Microscopy-at-Microscopy.com" {Microscopy-at-Microscopy.com} 27, 41 -- Subject: RE: [Microscopy] RE: viaWWW:Resharpened knife quality 27, 41 -- Thread-Topic: [Microscopy] RE: viaWWW:Resharpened knife quality 27, 41 -- Thread-Index: AQHUygFYIRWqpUgZmEiUF1y8vjwHP6XqdIKN 27, 41 -- Date: Thu, 21 Feb 2019 16:46:48 +0000 27, 41 -- Message-ID: {1550767608480.16437-at-inra.fr} 27, 41 -- References: {201902211611.x1LGBWFd022081-at-microscopy.com} 27, 41 -- In-Reply-To: {201902211611.x1LGBWFd022081-at-microscopy.com} 27, 41 -- Accept-Language: fr-FR, en-US 27, 41 -- Content-Language: fr-FR 27, 41 -- X-MS-Has-Attach: 27, 41 -- X-MS-TNEF-Correlator: 27, 41 -- x-ms-exchange-transport-fromentityheader: Hosted 27, 41 -- x-originating-ip: [138.102.156.18] 27, 41 -- Content-Type: text/plain; charset="iso-8859-1" 27, 41 -- MIME-Version: 1.0 27, 41 -- Content-Transfer-Encoding: 8bit 27, 41 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id x1LGc4fG027255 ==============================End of - Headers==============================
From roddorva8uoea-at-gmail.com Thu Feb 21 12:07:03 2019 Return-Path: {roddorva8uoea-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.161.138] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x1LI71sr005342 for {microscopylistserverarchive7-at-microscopy.com} ; Thu, 21 Feb 2019 12:07:02 -0600 Received: from qnx.mdrost.com ([7.52.177.47]) by relay.2yahoo.com with NNFMP; Thu, 21 Feb 2019 12:58:57 -0500 Message-ID: {522F0691.85DEE73B-at-gmail.com}
Dear All,
.... I am absolutely astonished by the (few) answers/comments from "disappointed"Â users of Diatome knifes.
I bought, inherited and used regularly and intensively new and resharpened diamond knifes from Diatome ( TEM and semithin knifes) from 1975 to 2018 ( i.e. for over 40 years),
all in all I estimate that more than 20 knifes went through my hands and to sum it up:Â ALL were perfect without the smallest fault !
I can't understand the complaints when using a new knive in detecting "bad" areas, when I received a new or resharpened knive from Diatome I immediately inspected the edge at the highest possible magnification at my cutting machine ( Reichert Ultracut ), in my opinion any flaw(s) should have become visible in the "dark field" mode of its binocular, and, as said, there were never any flaws in praxi ...
by the way: one time I privately bought at ebay-USA a "new and unused" Diatome knive, when receiving it the seal of the storage box was broken and the knife was dull and useless...
although by the way: all my (few) mail and phone contacts with Diatome in Switzerland were positive and helpful.
greetings,
Peter Heimann
( Cell Biology, Univ. of Bielefeld, Germany;Â retired in 2017 )
==============================Original Headers============================== 12, 20 -- From peter.heimann-at-uni-bielefeld.de Thu Feb 21 13:29:27 2019 12, 20 -- Received: from smtp.uni-bielefeld.de (uhrz-exch-pmb10.ad.uni-bielefeld.de [129.70.208.136]) 12, 20 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x1LJTQFs010471 12, 20 -- for {Microscopy-at-microscopy.com} ; Thu, 21 Feb 2019 13:29:27 -0600 12, 20 -- Received: from [192.168.188.34] (188.154.212.144) by 12, 20 -- uhrz-exch-pmb10.ad.uni-bielefeld.de (129.70.208.136) with Microsoft SMTP 12, 20 -- Server (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_128_GCM_SHA256) id 12, 20 -- 15.1.1591.10; Thu, 21 Feb 2019 20:38:11 +0100 12, 20 -- To: {Microscopy-at-microscopy.com} 12, 20 -- From: Peter Heimann {peter.heimann-at-uni-bielefeld.de} 12, 20 -- Subject: Re: re-sharpened knife ( Peter Heimann ) 12, 20 -- Message-ID: {c807d7d6-6e27-1ade-ec6c-dfa0acc3148c-at-uni-bielefeld.de} 12, 20 -- Date: Thu, 21 Feb 2019 20:38:10 +0100 12, 20 -- User-Agent: Mozilla/5.0 (Windows NT 10.0; WOW64; rv:60.0) Gecko/20100101 12, 20 -- Thunderbird/60.5.1 12, 20 -- MIME-Version: 1.0 12, 20 -- Content-Type: text/plain; charset="utf-8"; format=flowed 12, 20 -- Content-Transfer-Encoding: 8bit 12, 20 -- X-ClientProxiedBy: uhrz-exch-pmb01.ad.uni-bielefeld.de (129.70.208.127) To 12, 20 -- uhrz-exch-pmb10.ad.uni-bielefeld.de (129.70.208.136) ==============================End of - Headers==============================
Yes, I've also had lots of problems with Diatome diamond knives recently. We've had several resharpened and they produced knife marks and some areas on new knives are no better. I haven't complained to Diatome (but I should have done that). I, too, have been a loyal customer for 30+ years. It is sad to see the quality of their knives go to hell. They were always the best knives on the market. I now spend time apologizing to customers about the knife marks and re-section if necessary. I've even thought about making glass knives again (horrors!) but I'm too spoiled to go that route.
Hello all -- I don't have as much experience as many here (I've only been doing ultramicrotomy for 15 years or so) but my experience with Diatome knives has been the same as Peter Heimann's. I've used 3 Diatomes (all acquired new in different labs, the first probably in 2003 and the most recent in 2014), and they've all been flawless. The first of these was resharpened by them after years of use (in 2010, I think), and came back similarly flawless.
In a rare moment of more-than-adequate funding, I also acquired a MicroStar knife at the same time as the most recent Diatome. The quality of the MicroStar diamond edge has been just as good the Diatomes. However, the MicroStar's diamond was not set as precisely as any of the Diatomes. To be specific, the diamond is very slightly (a few micrometres) inset, such that in some cases the edges of the knife cannot be used without the specimen hitting the trough. (I apologise here: it's a bit hard to describe exactly how that works, but it is a problem.) The Diatomes I've used have never had this problem.
To be fair, I haven't contacted MicroStar about this issue; I don't know how common this problem is, or what their response would be. In my case, getting the problem fixed hasn't been necessary, since as long as I have two good diamond knives available, I want to use one for large survey sections (~1.2-1.5 mm wide, 70 nm thick) and one for much smaller and more delicate serial sections (~300 um wide, 50 nm thick). The MicroStar's fitting issues aren't a problem for survey-section work, while the Diatome's perfectly-set diamond is better for the serial work.
But most relevantly to this thread, and just to reiterate, I've had no reason to complain about Diatome knives, and have found them to be comparable in diamond and superior in setting to MicroStar's.
Aaron A. Heiss, Ph.D. Research Scientist and Simons Foundation Fellow Eunsoo Kim Laboratory Department of Invertebrate Zoology American Museum of Natural History Central Park West at 79th Street New York, NY 10024 USA
________________________________________ X-from: peter.heimann-at-uni-bielefeld.de {peter.heimann-at-uni-bielefeld.de} Sent: 21 February 2019 14:31 To: Aaron Heiss
Dear All,
.... I am absolutely astonished by the (few) answers/comments from "disappointed" users of Diatome knifes.
I bought, inherited and used regularly and intensively new and resharpened diamond knifes from Diatome ( TEM and semithin knifes) from 1975 to 2018 ( i.e. for over 40 years),
all in all I estimate that more than 20 knifes went through my hands and to sum it up: ALL were perfect without the smallest fault !
I can't understand the complaints when using a new knive in detecting "bad" areas, when I received a new or resharpened knive from Diatome I immediately inspected the edge at the highest possible magnification at my cutting machine ( Reichert Ultracut ), in my opinion any flaw(s) should have become visible in the "dark field" mode of its binocular, and, as said, there were never any flaws in praxi ...
by the way: one time I privately bought at ebay-USA a "new and unused" Diatome knive, when receiving it the seal of the storage box was broken and the knife was dull and useless...
although by the way: all my (few) mail and phone contacts with Diatome in Switzerland were positive and helpful.
greetings,
Peter Heimann
( Cell Biology, Univ. of Bielefeld, Germany; retired in 2017 )
==============================Original Headers============================== 12, 20 -- From peter.heimann-at-uni-bielefeld.de Thu Feb 21 13:29:27 2019 12, 20 -- Received: from smtp.uni-bielefeld.de (uhrz-exch-pmb10.ad.uni-bielefeld.de [129.70.208.136]) 12, 20 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x1LJTQFs010471 12, 20 -- for {Microscopy-at-microscopy.com} ; Thu, 21 Feb 2019 13:29:27 -0600 12, 20 -- Received: from [192.168.188.34] (188.154.212.144) by 12, 20 -- uhrz-exch-pmb10.ad.uni-bielefeld.de (129.70.208.136) with Microsoft SMTP 12, 20 -- Server (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_128_GCM_SHA256) id 12, 20 -- 15.1.1591.10; Thu, 21 Feb 2019 20:38:11 +0100 12, 20 -- To: {Microscopy-at-microscopy.com} 12, 20 -- From: Peter Heimann {peter.heimann-at-uni-bielefeld.de} 12, 20 -- Subject: Re: re-sharpened knife ( Peter Heimann ) 12, 20 -- Message-ID: {c807d7d6-6e27-1ade-ec6c-dfa0acc3148c-at-uni-bielefeld.de} 12, 20 -- Date: Thu, 21 Feb 2019 20:38:10 +0100 12, 20 -- User-Agent: Mozilla/5.0 (Windows NT 10.0; WOW64; rv:60.0) Gecko/20100101 12, 20 -- Thunderbird/60.5.1 12, 20 -- MIME-Version: 1.0 12, 20 -- Content-Type: text/plain; charset="utf-8"; format=flowed 12, 20 -- Content-Transfer-Encoding: 8bit 12, 20 -- X-ClientProxiedBy: uhrz-exch-pmb01.ad.uni-bielefeld.de (129.70.208.127) To 12, 20 -- uhrz-exch-pmb10.ad.uni-bielefeld.de (129.70.208.136) ==============================End of - Headers==============================
indeed ( I did not comment on this) is the fine positioning of the diamond within the aluminum trough and the resin "glue" perfect whereas two other knifes from different maufacturers made problems in this respect when trying to cut difficult positioned re-embedded specimen blocks ( i.e. the block surface made contact with the resin cement before contact with the diamond) ...
another (positive) characteristic point is that the knife edge of Diatome knifes (at least in my hands) allowed for the water level to be lowered extremely without "breaking away" or "ripping off" from the knife edge and this pleasant feature was consistent over total life-time
best regards,
Peter ( Heimann )
#####################################
Am 21.02.2019 um 22:07 schrieb aheiss-at-amnh.org: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello all -- I don't have as much experience as many here (I've only been doing ultramicrotomy for 15 years or so) but my experience with Diatome knives has been the same as Peter Heimann's. I've used 3 Diatomes (all acquired new in different labs, the first probably in 2003 and the most recent in 2014), and they've all been flawless. The first of these was resharpened by them after years of use (in 2010, I think), and came back similarly flawless. } } In a rare moment of more-than-adequate funding, I also acquired a MicroStar knife at the same time as the most recent Diatome. The quality of the MicroStar diamond edge has been just as good the Diatomes. However, the MicroStar's diamond was not set as precisely as any of the Diatomes. To be specific, the diamond is very slightly (a few micrometres) inset, such that in some cases the edges of the knife cannot be used without the specimen hitting the trough. (I apologise here: it's a bit hard to describe exactly how that works, but it is a problem.) The Diatomes I've used have never had this problem. } } To be fair, I haven't contacted MicroStar about this issue; I don't know how common this problem is, or what their response would be. In my case, getting the problem fixed hasn't been necessary, since as long as I have two good diamond knives available, I want to use one for large survey sections (~1.2-1.5 mm wide, 70 nm thick) and one for much smaller and more delicate serial sections (~300 um wide, 50 nm thick). The MicroStar's fitting issues aren't a problem for survey-section work, while the Diatome's perfectly-set diamond is better for the serial work. } } But most relevantly to this thread, and just to reiterate, I've had no reason to complain about Diatome knives, and have found them to be comparable in diamond and superior in setting to MicroStar's. } } } Aaron A. Heiss, Ph.D. } Research Scientist and Simons Foundation Fellow } Eunsoo Kim Laboratory } Department of Invertebrate Zoology } American Museum of Natural History } Central Park West at 79th Street } New York, NY 10024 USA } } phone: 212-769-5838 } fax: 212-769-5277 } aheiss-at-amnh.org } } } ________________________________________ } X-from: peter.heimann-at-uni-bielefeld.de {peter.heimann-at-uni-bielefeld.de} } Sent: 21 February 2019 14:31 } To: Aaron Heiss } Subject: [Microscopy] Re: re-sharpened knife ( Peter Heimann ) } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.microscopy.com%2FMicroscopyListserver&data=01%7C01%7Caheiss%40amnh.org%7C171a4294cc90422e334108d698346589%7Cbe0003e8c6b9496883aeb34586974b76%7C0&sdata=Tt6V3Sb4FFAeZT2QrlwsJdFCdQ5UkzmWzCpiEvZvjUE%3D&reserved=0 } On-Line Help https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.microscopy.com%2FMicroscopyListserver%2FFAQ.html&data=01%7C01%7Caheiss%40amnh.org%7C171a4294cc90422e334108d698346589%7Cbe0003e8c6b9496883aeb34586974b76%7C0&sdata=8RWFdlgM637Qc1fYxvG84LyulJ25ZmpFLo%2FFIqPENXU%3D&reserved=0 } ---------------------------------------------------------------------------- } } Dear All, } } .... I am absolutely astonished by the (few) answers/comments from } "disappointed"ďż˝ users of Diatome knifes. } } I bought, inherited and used regularly and intensively new and } resharpened diamond knifes from Diatome ( TEM and semithin knifes) from } 1975 to 2018 ( i.e. for over 40 years), } } all in all I estimate that more than 20 knifes went through my hands and } to sum it up: ALL were perfect without the smallest fault ! } } I can't understand the complaints when using a new knive in detecting } "bad" areas, when I received a new or resharpened knive from Diatome I } immediately inspected the edge at the highest possible magnification at } my cutting machine ( Reichert Ultracut ), in my opinion any flaw(s) } should have become visible in the "dark field" mode of its binocular, } and, as said, there were never any flaws in praxi ... } } by the way: one time I privately bought at ebay-USA a "new and unused" } Diatome knive, when receiving it the seal of the storage box was broken } and the knife was dull and useless... } } although by the way: all my (few) mail and phone contacts with Diatome } in Switzerland were positive and helpful. } } greetings, } } Peter Heimann } } ( Cell Biology, Univ. of Bielefeld, Germany; retired in 2017 ) } } } } ==============================Original Headers============================== } 12, 20 -- From peter.heimann-at-uni-bielefeld.de Thu Feb 21 13:29:27 2019 } 12, 20 -- Received: from smtp.uni-bielefeld.de (uhrz-exch-pmb10.ad.uni-bielefeld.de [129.70.208.136]) } 12, 20 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x1LJTQFs010471 } 12, 20 -- for {Microscopy-at-microscopy.com} ; Thu, 21 Feb 2019 13:29:27 -0600 } 12, 20 -- Received: from [192.168.188.34] (188.154.212.144) by } 12, 20 -- uhrz-exch-pmb10.ad.uni-bielefeld.de (129.70.208.136) with Microsoft SMTP } 12, 20 -- Server (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_128_GCM_SHA256) id } 12, 20 -- 15.1.1591.10; Thu, 21 Feb 2019 20:38:11 +0100 } 12, 20 -- To: {Microscopy-at-microscopy.com} } 12, 20 -- From: Peter Heimann {peter.heimann-at-uni-bielefeld.de} } 12, 20 -- Subject: Re: re-sharpened knife ( Peter Heimann ) } 12, 20 -- Message-ID: {c807d7d6-6e27-1ade-ec6c-dfa0acc3148c-at-uni-bielefeld.de} } 12, 20 -- Date: Thu, 21 Feb 2019 20:38:10 +0100 } 12, 20 -- User-Agent: Mozilla/5.0 (Windows NT 10.0; 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==============================Original Headers============================== 7, 22 -- From peter.heimann-at-uni-bielefeld.de Thu Feb 21 15:24:51 2019 7, 22 -- Received: from smtp.uni-bielefeld.de (uhrz-exch-pmb10.ad.uni-bielefeld.de [129.70.208.136]) 7, 22 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x1LLOogG001516 7, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 21 Feb 2019 15:24:51 -0600 7, 22 -- Received: from [192.168.188.34] (188.154.212.144) by 7, 22 -- uhrz-exch-pmb10.ad.uni-bielefeld.de (129.70.208.136) with Microsoft SMTP 7, 22 -- Server (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_128_GCM_SHA256) id 7, 22 -- 15.1.1591.10; Thu, 21 Feb 2019 22:33:35 +0100 7, 22 -- Subject: Re: [Microscopy] re-sharpened knife ( Peter Heimann ) 7, 22 -- To: {aheiss-at-amnh.org} , {Microscopy-at-microscopy.com} 7, 22 -- References: {201902212107.x1LL7bQh030268-at-microscopy.com} 7, 22 -- From: Peter Heimann {peter.heimann-at-uni-bielefeld.de} 7, 22 -- Message-ID: {59ea9564-3771-8c34-45e7-52035c058e37-at-uni-bielefeld.de} 7, 22 -- Date: Thu, 21 Feb 2019 22:33:34 +0100 7, 22 -- User-Agent: Mozilla/5.0 (Windows NT 10.0; WOW64; rv:60.0) Gecko/20100101 7, 22 -- Thunderbird/60.5.1 7, 22 -- MIME-Version: 1.0 7, 22 -- In-Reply-To: {201902212107.x1LL7bQh030268-at-microscopy.com} 7, 22 -- Content-Type: text/plain; charset="utf-8"; format=flowed 7, 22 -- Content-Transfer-Encoding: 8bit 7, 22 -- X-ClientProxiedBy: uhrz-exch-pmb09.ad.uni-bielefeld.de (129.70.208.135) To 7, 22 -- uhrz-exch-pmb10.ad.uni-bielefeld.de (129.70.208.136) ==============================End of - Headers==============================
Just to add - we have used new and resharpened Diatome knives for 30+ years with no complaint at all. We send our knives back to Diatome for resharpening through an excellent and reliable agent here in Australia.
I don't disbelieve the dissatisfied people, but I am surprised, this is quite different from our experience.
cheers, Rosemary
Dr Rosemary White CSIRO Black Mountain GPO Box 1700 Canberra, ACT 2601 Australia
T 61 2 6246 5475 M 61 468 966 713 ________________________________________ X-from: peter.heimann-at-uni-bielefeld.de [peter.heimann-at-uni-bielefeld.de] Sent: Friday, 22 February 2019 8:25 a.m. To: White, Rosemary (A&F, Black Mountain)
thanks Aaron for your comment!
indeed ( I did not comment on this) is the fine positioning of the diamond within the aluminum trough and the resin "glue" perfect whereas two other knifes from different maufacturers made problems in this respect when trying to cut difficult positioned re-embedded specimen blocks ( i.e. the block surface made contact with the resin cement before contact with the diamond) ...
another (positive) characteristic point is that the knife edge of Diatome knifes (at least in my hands) allowed for the water level to be lowered extremely without "breaking away" or "ripping off" from the knife edge and this pleasant feature was consistent over total life-time
best regards,
Peter ( Heimann )
#####################################
Am 21.02.2019 um 22:07 schrieb aheiss-at-amnh.org: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello all -- I don't have as much experience as many here (I've only been doing ultramicrotomy for 15 years or so) but my experience with Diatome knives has been the same as Peter Heimann's. I've used 3 Diatomes (all acquired new in different labs, the first probably in 2003 and the most recent in 2014), and they've all been flawless. The first of these was resharpened by them after years of use (in 2010, I think), and came back similarly flawless. } } In a rare moment of more-than-adequate funding, I also acquired a MicroStar knife at the same time as the most recent Diatome. The quality of the MicroStar diamond edge has been just as good the Diatomes. However, the MicroStar's diamond was not set as precisely as any of the Diatomes. To be specific, the diamond is very slightly (a few micrometres) inset, such that in some cases the edges of the knife cannot be used without the specimen hitting the trough. (I apologise here: it's a bit hard to describe exactly how that works, but it is a problem.) The Diatomes I've used have never had this problem. } } To be fair, I haven't contacted MicroStar about this issue; I don't know how common this problem is, or what their response would be. In my case, getting the problem fixed hasn't been necessary, since as long as I have two good diamond knives available, I want to use one for large survey sections (~1.2-1.5 mm wide, 70 nm thick) and one for much smaller and more delicate serial sections (~300 um wide, 50 nm thick). The MicroStar's fitting issues aren't a problem for survey-section work, while the Diatome's perfectly-set diamond is better for the serial work. } } But most relevantly to this thread, and just to reiterate, I've had no reason to complain about Diatome knives, and have found them to be comparable in diamond and superior in setting to MicroStar's. } } } Aaron A. Heiss, Ph.D. } Research Scientist and Simons Foundation Fellow } Eunsoo Kim Laboratory } Department of Invertebrate Zoology } American Museum of Natural History } Central Park West at 79th Street } New York, NY 10024 USA } } phone: 212-769-5838 } fax: 212-769-5277 } aheiss-at-amnh.org } } } ________________________________________ } X-from: peter.heimann-at-uni-bielefeld.de {peter.heimann-at-uni-bielefeld.de} } Sent: 21 February 2019 14:31 } To: Aaron Heiss } Subject: [Microscopy] Re: re-sharpened knife ( Peter Heimann ) } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.microscopy.com%2FMicroscopyListserver&data=01%7C01%7Caheiss%40amnh.org%7C171a4294cc90422e334108d698346589%7Cbe0003e8c6b9496883aeb34586974b76%7C0&sdata=Tt6V3Sb4FFAeZT2QrlwsJdFCdQ5UkzmWzCpiEvZvjUE%3D&reserved=0 } On-Line Help https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.microscopy.com%2FMicroscopyListserver%2FFAQ.html&data=01%7C01%7Caheiss%40amnh.org%7C171a4294cc90422e334108d698346589%7Cbe0003e8c6b9496883aeb34586974b76%7C0&sdata=8RWFdlgM637Qc1fYxvG84LyulJ25ZmpFLo%2FFIqPENXU%3D&reserved=0 } ---------------------------------------------------------------------------- } } Dear All, } } .... I am absolutely astonished by the (few) answers/comments from } "disappointed"ďż˝ users of Diatome knifes. } } I bought, inherited and used regularly and intensively new and } resharpened diamond knifes from Diatome ( TEM and semithin knifes) from } 1975 to 2018 ( i.e. for over 40 years), } } all in all I estimate that more than 20 knifes went through my hands and } to sum it up: ALL were perfect without the smallest fault ! } } I can't understand the complaints when using a new knive in detecting } "bad" areas, when I received a new or resharpened knive from Diatome I } immediately inspected the edge at the highest possible magnification at } my cutting machine ( Reichert Ultracut ), in my opinion any flaw(s) } should have become visible in the "dark field" mode of its binocular, } and, as said, there were never any flaws in praxi ... } } by the way: one time I privately bought at ebay-USA a "new and unused" } Diatome knive, when receiving it the seal of the storage box was broken } and the knife was dull and useless... } } although by the way: all my (few) mail and phone contacts with Diatome } in Switzerland were positive and helpful. } } greetings, } } Peter Heimann } } ( Cell Biology, Univ. of Bielefeld, Germany; retired in 2017 ) } } } } ==============================Original Headers============================== } 12, 20 -- From peter.heimann-at-uni-bielefeld.de Thu Feb 21 13:29:27 2019 } 12, 20 -- Received: from smtp.uni-bielefeld.de (uhrz-exch-pmb10.ad.uni-bielefeld.de [129.70.208.136]) } 12, 20 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x1LJTQFs010471 } 12, 20 -- for {Microscopy-at-microscopy.com} ; Thu, 21 Feb 2019 13:29:27 -0600 } 12, 20 -- Received: from [192.168.188.34] (188.154.212.144) by } 12, 20 -- uhrz-exch-pmb10.ad.uni-bielefeld.de (129.70.208.136) with Microsoft SMTP } 12, 20 -- Server (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_128_GCM_SHA256) id } 12, 20 -- 15.1.1591.10; Thu, 21 Feb 2019 20:38:11 +0100 } 12, 20 -- To: {Microscopy-at-microscopy.com} } 12, 20 -- From: Peter Heimann {peter.heimann-at-uni-bielefeld.de} } 12, 20 -- Subject: Re: re-sharpened knife ( Peter Heimann ) } 12, 20 -- Message-ID: {c807d7d6-6e27-1ade-ec6c-dfa0acc3148c-at-uni-bielefeld.de} } 12, 20 -- Date: Thu, 21 Feb 2019 20:38:10 +0100 } 12, 20 -- User-Agent: Mozilla/5.0 (Windows NT 10.0; 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==============================Original Headers============================== 7, 22 -- From peter.heimann-at-uni-bielefeld.de Thu Feb 21 15:24:51 2019 7, 22 -- Received: from smtp.uni-bielefeld.de (uhrz-exch-pmb10.ad.uni-bielefeld.de [129.70.208.136]) 7, 22 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x1LLOogG001516 7, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 21 Feb 2019 15:24:51 -0600 7, 22 -- Received: from [192.168.188.34] (188.154.212.144) by 7, 22 -- uhrz-exch-pmb10.ad.uni-bielefeld.de (129.70.208.136) with Microsoft SMTP 7, 22 -- Server (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_128_GCM_SHA256) id 7, 22 -- 15.1.1591.10; Thu, 21 Feb 2019 22:33:35 +0100 7, 22 -- Subject: Re: [Microscopy] re-sharpened knife ( Peter Heimann ) 7, 22 -- To: {aheiss-at-amnh.org} , {Microscopy-at-microscopy.com} 7, 22 -- References: {201902212107.x1LL7bQh030268-at-microscopy.com} 7, 22 -- From: Peter Heimann {peter.heimann-at-uni-bielefeld.de} 7, 22 -- Message-ID: {59ea9564-3771-8c34-45e7-52035c058e37-at-uni-bielefeld.de} 7, 22 -- Date: Thu, 21 Feb 2019 22:33:34 +0100 7, 22 -- User-Agent: Mozilla/5.0 (Windows NT 10.0; WOW64; rv:60.0) Gecko/20100101 7, 22 -- Thunderbird/60.5.1 7, 22 -- MIME-Version: 1.0 7, 22 -- In-Reply-To: {201902212107.x1LL7bQh030268-at-microscopy.com} 7, 22 -- Content-Type: text/plain; charset="utf-8"; format=flowed 7, 22 -- Content-Transfer-Encoding: 8bit 7, 22 -- X-ClientProxiedBy: uhrz-exch-pmb09.ad.uni-bielefeld.de (129.70.208.135) To 7, 22 -- uhrz-exch-pmb10.ad.uni-bielefeld.de (129.70.208.136) ==============================End of - Headers==============================
I would like to add my "official" comments on the knife matter. I am not upset with Diatome or EMS as they have always bent over backwards to make right any problem I've had, and most often to their vast expense. But back to the issue of diamond knives.
Yes I have had problems with Diatome knives from time to time, but with diligence Diatome has always re-sharpened or replaced my knives no problem. At one point a few months ago I still had very confusing problems with brand new knives being scored or damaged seemingly as opened or after using only one time. It looked like they were shipped to me that way. I even sent images to Diatome and EMS to show the scraping and marks I was getting. Helmut Gnaegi came all the way from Switzerland just to visit our lab and check the issues with the diamond knives we were experiencing.
It turned out after an hour or so of testing different samples and parameters, the clearance angle on my microtome was not accurate. The factory settings had been tweaked somehow. The clearance angle was no longer correct at the setting it showed. So instead of the knife clearance angle being 6 degrees, it was actually more like 3 degrees. That in itself would cause major scraping all along the knife and also damage the knife edge. We had to adjust the knife clearance angle up to 9 degrees in order for the water level and knife to cut properly. None of us would have thought that the clearance angle setting would be off. It's not something that I change. Helmut noticed it when he looked at the boat. The water was not level as it should be.
In the Leica UC7 microtome, the lighting is so good that I didn't even notice a difference in the water level lines. I was actually still able to cut at the wrong angle it just damaged the knife right away. Thereby making me think that I received damaged or insufficiently sharpened knives for months. Since Helmut's visit and the clearance angle revelation, I've had no other issues with the scraping or heavy knife marks. I am grateful to Helmut and to EMS for his visit. I relayed this information to others I knew too in the field. Whether they used it or not I don't know.
We work extensively with Drosophila tissue. The exoskeleton makes for us many problems in sectioning. So we know that we have to use Histo knives for making light slides, and only Ultra knives for TEM imaging. The two knives are sharpened differently it seems. We even have to request deeper sharpening at times which has helped. Diatome has had their own issues on the industrial level that affect manufacturing too. It's just the nature of this intricate business. For us, we cut up dead things, and dead things can be hard to deal with at times!
My advice to others is that if you are experiencing problems, take pictures, send micrographs, explain what is happening and systematically investigate the issue. Then perhaps an agreement can be made that will be profitable for both sides.
Thank you,
Lita Duraine Certified TEM Specialist HHMI- Molecular Genetics Duncan Neurological Research Institute Baylor College of Medicine 1250 Moursund St. Houston, TX 77030 Office: 832-824-8704 MS: N1125.50
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I am surprised at the current thread of complaints about Diatome Diamond knives. I have been in EM since the early 1970's and have used many different brands of diamond knives, some of which are no longer around. When I took over my current facility in 2001, I had the resources to finally buy Diatome knives which I did in a heartbeat. They are the best. Here in the U.S. Stacie Kirsch owner of Electron Microscopy Sciences will make sure things are corrected, if you have a problem. I am not saying the people having bad experiences are to be discounted, but I hope they all have tried to work with the vendor. I also caution about airing complaints in a public forum such as the MSA listserver. Like any public medium these days one needs to be careful of what is said. I have asked for feedback on products, but have asked that people respond privately. Remember, what is said can be misconstrued and there is always two sides (sometimes more) to a story.
Tom Bargar UNMC Electron Microscopy Core Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
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My apologies to Diatome for my earlier post. I should have consulted with them as Lita Duraine did and worked out the knife issues. Her post was very helpful/informative. I will definitely test the knife angle on the ultramicrotome (it is never taken off 6 degrees).
Beth Richardson, Georgia Electron Microscopy
X-from: duraine-at-bcm.edu {duraine-at-bcm.edu} Sent: Thursday, February 21, 2019 5:04 PM To: Beth Richardson
Hello all,
I would like to add my "official" comments on the knife matter. I am not upset with Diatome or EMS as they have always bent over backwards to make right any problem I've had, and most often to their vast expense. But back to the issue of diamond knives.
Yes I have had problems with Diatome knives from time to time, but with diligence Diatome has always re-sharpened or replaced my knives no problem. At one point a few months ago I still had very confusing problems with brand new knives being scored or damaged seemingly as opened or after using only one time. It looked like they were shipped to me that way. I even sent images to Diatome and EMS to show the scraping and marks I was getting. Helmut Gnaegi came all the way from Switzerland just to visit our lab and check the issues with the diamond knives we were experiencing.
It turned out after an hour or so of testing different samples and parameters, the clearance angle on my microtome was not accurate. The factory settings had been tweaked somehow. The clearance angle was no longer correct at the setting it showed. So instead of the knife clearance angle being 6 degrees, it was actually more like 3 degrees. That in itself would cause major scraping all along the knife and also damage the knife edge. We had to adjust the knife clearance angle up to 9 degrees in order for the water level and knife to cut properly. None of us would have thought that the clearance angle setting would be off. It's not something that I change. Helmut noticed it when he looked at the boat. The water was not level as it should be.
In the Leica UC7 microtome, the lighting is so good that I didn't even notice a difference in the water level lines. I was actually still able to cut at the wrong angle it just damaged the knife right away. Thereby making me think that I received damaged or insufficiently sharpened knives for months. Since Helmut's visit and the clearance angle revelation, I've had no other issues with the scraping or heavy knife marks. I am grateful to Helmut and to EMS for his visit. I relayed this information to others I knew too in the field. Whether they used it or not I don't know.
We work extensively with Drosophila tissue. The exoskeleton makes for us many problems in sectioning. So we know that we have to use Histo knives for making light slides, and only Ultra knives for TEM imaging. The two knives are sharpened differently it seems. We even have to request deeper sharpening at times which has helped. Diatome has had their own issues on the industrial level that affect manufacturing too. It's just the nature of this intricate business. For us, we cut up dead things, and dead things can be hard to deal with at times!
My advice to others is that if you are experiencing problems, take pictures, send micrographs, explain what is happening and systematically investigate the issue. Then perhaps an agreement can be made that will be profitable for both sides.
Thank you,
Lita Duraine Certified TEM Specialist HHMI- Molecular Genetics Duncan Neurological Research Institute Baylor College of Medicine 1250 Moursund St. Houston, TX 77030 Office: 832-824-8704 MS: N1125.50
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Email: slockledge-at-tiptek.com Name: Scott P Lockledge
Organization: Tiptek, LLC
Title-Subject: [Filtered] Zeiss SEM Joystick Controller Lubricants
Message: Recently, we have experienced the joystick of a Zeiss SEM controller "sticking" and not releasing. The Zeiss service representative suggested trying a lubricant, but had no specific suggestions. I found this one online to be used successfully by gamers: Dow Corning Molykote 44 Medium Grease Lubricant.
Any experience with "sticking" joysticks, cleaning, or lubrication of them? Any suggestions would be appreciated.
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Email: leunissen-at-xtra.co.nz Name: Jan Leunissen
I have been following the correspondence re diamond knife issues and I feel the need to add a few words. No doubt mistakes are made, by everyone, everywhere and all the time, and like Rosemary White wrote, it is certainly not such that I “disbelieve the dissatisfied people". Being in business myself I value criticism as it gives people a chance to seriously look into issues and see where improvements can be made or how users/scientists can be supported to get better results. Feedback is key. But also: negative public messages can easily make a big dent in a company’s reputation, deservedly or not. Diatome to almost all of us is Helmut Gnaegi, If there is one person in business who really does everything he can to solve issues, to help research projects, then I would have to say it is Helmut. As far as experience is concerned: his 50th work anniversary with Diatome was just 3 weeks ago.
I admit, we do have some commercial interest in Diatome, since we proudly represent them in The Netherlands but there is more than business, there is also friendship and compassion.
I would urge everyone with questions or comments to contact Helmut, he is very approachable and will leave no stone unturned.
Jan Leunissen Dunedin, New Zealand
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I would say to be careful and use very sparingly as a "wet" lube will just attract more dirt/dust which will end up further gumming things up. In the past on an Amray I used a Teflon based dry lubricant and a paraffin wax (not together). Have not had the issue on my Zeiss yet but would do the same if it came up. We clean it and dust weekly to prevent the problem and it seems to be working at least since 2011 when we bought her. Considered a graphite on the Amray but was wary of electrically bridging contacts as I didn't know how the controller worked at the time.
Scott Whittaker Lab Manager Imaging P.O. Box 37012 MRC-104 Room W150 Washington, DC 20013-7012 w 202.633.0891 whittaks-at-si.edu
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-----Original Message----- X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Thursday, February 21, 2019 8:01 PM To: Whittaker, Scott {WHITTAKS-at-si.edu}
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X-from: slockledge-at-tiptek.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both slockledge-at-tiptek.com, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Email: slockledge-at-tiptek.com Name: Scott P Lockledge
Organization: Tiptek, LLC
Title-Subject: [Filtered] Zeiss SEM Joystick Controller Lubricants
Message: Recently, we have experienced the joystick of a Zeiss SEM controller "sticking" and not releasing. The Zeiss service representative suggested trying a lubricant, but had no specific suggestions. I found this one online to be used successfully by gamers: Dow Corning Molykote 44 Medium Grease Lubricant.
Any experience with "sticking" joysticks, cleaning, or lubrication of them? Any suggestions would be appreciated.
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CeB6 is a little bit more expensive than a LaB6, this would be the only disadvantage I could think about… It is less sensitive to contamination than LaB6, yet you will still need a clean ultra high-vacuum in the gun (although not as low as a FEG). CeB6 has the advantage that it requires a lower evaporation temperature and it has a lower work function (2.7 eV for LaB6 vs 2.4 eV for CeB6). Therefore, you will get a longer lifetime of CeB6 over LaB6, and a slightly better brightness.
If you can afford a CeB6, go for it! You can also contact Mike Jercinovic at UMass Amherst (USA), he has more experience than I do with LaB6 vs CeB6 - I actually got all the information listed above from him when I was a postdoc at UMass Amherst :)
Julien
########################### *Dr. Julien Allaz *Head assistant for SEM/EPMA Inst. fĂĽr Geochemie und Petrologie ETH ZĂĽrich NWÂ E 84 Clausiusstrasse 25 8092Â ZĂĽrich Switzerland
} On 21 Feb 2019, at 22:51, microscopy.listserver-at-gmail.com } {mailto:microscopy.listserver-at-gmail.com} wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America } To  Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } } } -------- Forwarded Message -------- } } Date: Thu, 21 Feb 2019 08:29:35 -0300 } X-from: Erico Freitas {ericotadeu-at-ufmg.br {mailto:ericotadeu-at-ufmg.br} } } } } } Dear all, } } } I would like to hear from the pros and cons of the cathode CeB6 in } comparison to the LaB6? } } } Kind regards, } } -- } Erico Freitas } } Physicist/Microscopist at Center of Microscopy } Universidade Federal de Minas Gerais (UFMG) } Av. 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From solizbula031ioi-at-gmail.com Fri Feb 22 21:38:46 2019 Return-Path: {solizbula031ioi-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.161.136] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x1N3cjXq017563 for {microscopylistserverarchive7-at-microscopy.com} ; Fri, 22 Feb 2019 21:38:46 -0600 Received: from unknown (HELO mxs.perenter.com) (Sat, 23 Feb 2019 07:38:43 +0400) by smtp4.cyberemailings.com with QMQP; Sat, 23 Feb 2019 07:38:43 +0400 Received: from unknown (195.134.152.5) by mail.webhostings4u.com with QMQP; Sat, 23 Feb 2019 07:34:42 +0400 Received: from unknown (HELO webmail.halftomorrow.com) (Sat, 23 Feb 2019 07:20:46 +0400) by m1.gns.snv.thisdomainl.com with LOCAL; Sat, 23 Feb 2019 07:20:46 +0400 Message-ID: {EA958D7E.E33F5042-at-gmail.com}
Arunas,
In addition to the HV alignment that you did, I am going to assume that you did the whole alignment for the JEOL 2100F. If you don't have the full alignment procedure, send me an email at scott dot d dot walck2 dot ctr at mail dot mil and I will send you my alignment procedure that I require every user to perform at the beginning of their session.
Follow these steps to get your SAED pattern on the phosphor screen(s): 1) In TEM mode without the SAD aperture in (you can also do this without the Obj aperture in), hit the Std focus button. 2) Above 40kX (200kX is better), raise/lower the sample to get minimum contrast (Your sample is now focused and the diffraction pattern is formed in the back focal plane of the objective lens.) Use a live FFT to help you find the minimum contrast. 3) Lower your TEM magnification to the desired value and center your sample area. 4) Put in the appropriate SAD aperture and center it. 5) Turn the Brightness Knob fully clockwise. If you can read the JEOLS Hex values, they should read "FFFF". Regardless, you will hear a beep when you have turned it fully clockwise. 6) Press the SA DIFF button and select the desired cameral length. 7) Center the Transmitted spot with the PLA button selected and using the Def controls. (This is the step that I think that you are missing in your procedure.) 8) Focus the Diffraction spot with Diff Focus (while using the binoculars). This is adjusting the Intermediate 1 lens to grab the back focal plane of the objective lens for the projector lens system. The back focal plane is where the diffraction pattern is formed. 9) If while focusing the IL1, you see astigmatism in the spot, correct it. You have to select IL stig on the TEMCON screen, there is no button for it on the control pads. Now this gets tricky. You have to turn the Dif Focus button continuously through focus while adjusting first the X DEF control and then the Y DEF control to get rid of the IL astigmatism while all the time watching through the binoculars. You can look like a monkey while doing it, so consider not having anybody around who might witness you doing it.
This is actually the basic procedure for any microscope, not just the JEOL 2100F (other than the names of the specific controls.) You should have done exactly this procedure one time for each camera length with your calibration standard (gold or aluminum polycrystalline evaporated films) and saved them in Digital Micrograph. You need to follow this procedure for every unknown sample so that you have duplicated what was done for your standard at the selected camera length. The key to success is reproducibility. You should have about a 1-2% error or better. Note: not only have I done this in DM for every camera length, I also use Dave Mitchell's DiffTools script and have calibrated all of the camera lengths in that as well. The good thing is that you can use the same patterns for both.
Recording patterns using a digital camera: 10) If you are using a digital camera to record the pattern, lower the exposure time to protect the camera. 11) In Digital Micrograph, draw two lines from corner to opposite corner to find the center of the micrograph. 12) Put the controls in PLA mode and get ready to quickly center the diffraction pattern by putting the transmitted spot at the center cross 13) Lift the screen and center the beam at the cross, QUICKLY. Then lower the screen. 14) Now, use the beam block and center it while using the binoculars. Note: if the beam block drifts on you, you need to call the service engineer and have him tighten it so that it is stiff and takes effort to move it. That way it will not move during collection of your diffraction pattern. 15) Lift the screen. If you are using DM and View with Bin3, put your exposure at about 0.1 sec. You may have to adjust the beam block carefully to make sure that any brightness around it is uniform on both sides. Do this very carefully so that you don't expose the camera to the full spot. 16) Using the mouse, read the intensity of the brightest spots to find the maximum intensity in the pattern by reading the values in DM. 17) Using the intensity that you found, increase the exposure until that spot that you found is just under the saturation point of the camera. 16-17 alternative) Write a DM script that finds the maximum value in the pattern and calculates the appropriate Record exposure. EASY TO DO. 18) Lower the exposure of the View to very low again. 19) Set your record exposure and take an image. Depending on what aperture you have in, this can be a few seconds to more than 60 seconds. Avoid the temptation to increase the brightness to get faster patterns. You want the brightness fully clockwise because you want the beam as parallel as possible.
This gives you a very good diffraction pattern image with the beam block in, but you don't have the center of the pattern.
On older machines, it was easy to double expose a pattern a second time with a shorter exposure and with the beam block out to record the transmitted spot. It is a little more involved to do it with a digital camera. 20) While you are recording the long exposure in DM, change the Record exposure to something like 0.02 to 0.08 s depending on how bright your transmitted spot is. 21) When the recording is finished, quickly remove the beam block (that is why you changed the exposure to a short one in the View) and press the Record button to take another pattern with the short exposure. 22) When it is finished, immediately press the F6 button (this should be the Beam Blank if it hasn't been changed from the default setting) to blank the beam, lower the screen, and then press F6 again to un-blank the beam. 23) You have two patterns now. Save, but do not close the first one. You have options how you want to use the second pattern with the transmitted spot. You can find the X,Y coordinate with the maximum intensity and note that on the first pattern. You can add the two patterns together. (If you do this, then you also have to copy the tags from the first pattern to the resultant image.) What I did was write a little script that selects and copies a center square from the second image that includes the transmitted spot and pastes it into the image of the first. I then add a "+" to the name and save it in the directory where the first image was saved. With the transmitted beam marked, it is very easy to center the pattern for diffraction analysis software.
I hope this helps. -Scott
-----Original Message----- X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Monday, February 18, 2019 8:12 PM To: s.walck-at-comcast.net
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X-from: arunas.mesceriakovas-at-uef.fi
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Title-Subject: [Filtered] Tips for TEM SAED JEOL 2100F
Message: Hello,
I would like to ask some practical tips on performing SAED on a JEOL 2100F TEM. I am a complete newbie to TEM diffraction and the "2100 user manual" diffraction chapter is rather limited in describing influence of various settings (or recommendation of it) on the outcome of the diffraction patterns i.e. use of condenser lenses, alpha, spot values, required/suggested alignment/correction procedures. I usually (1)focus on the sample in question at about 100kx, (2) perform voltage centering alignment (HT WOB), (3) WOB Y/X correction. And then switch to the SAED procedure in chapter 5.5.1 of the manual.
My samples are micron sized fly ash particles (silicates, aluminosilicates). Fixed on holey carbon films on copper mesh. The TEM is operated at 200kV. So far one of the most notable "problems" I have is: (1)After selecting/centering the area of interest on the fluorescent screen (SA MAG mode, field limiting aperture inserted) I switch to SA DIFF mode and the beam shifts position to the top left (midway radius) on the fluorescent screen which makes navigation on the sample very difficult. (2)Using the DIFF FOCUS knob to focus the beam into a small spot, the diffraction patterns do not appear completely spherical (mild ellipse).
Any practical tips/steps on setting up the scope for the best SAED experience would be greatly appreciated.
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Message: Hi, I am trying to cut retinal longitudinal sections with a glass knives on a brand new ultramicrotome. I am using both 6.4 mm and 10 mm thick glass. I get chatter and shredding with 70-90 nm, although the sections look better at 90 nm. When I do semi-thins for LM, the viewing is fine with Toluidine blue O staining. Any suggestions? Thanks! Vickie
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I would say to be careful and use very sparingly as a "wet" lube will just attract more dirt/dust which will end up further gumming things up. In the past on an Amray I used a Teflon based dry lubricant and a paraffin wax (not together). Have not had the issue on my Zeiss yet but would do the same if it came up. We clean it and dust weekly to prevent the problem and it seems to be working at least since 2011 when we bought her. Considered a graphite on the Amray but was wary of electrically bridging contacts as I didn't know how the controller worked at the time.
Scott Whittaker Lab Manager Imaging P.O. Box 37012 MRC-104 Room W150 Washington, DC 20013-7012 w 202.633.0891 whittaks-at-si.edu
SMITHSONIAN INSTITUTION NATIONAL MUSEUM OF NATURAL HISTORY Facebook | Twitter | Instagram
-----Original Message----- X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Thursday, February 21, 2019 8:01 PM To: Whittaker, Scott {WHITTAKS-at-si.edu}
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X-from: slockledge-at-tiptek.com
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Email: slockledge-at-tiptek.com Name: Scott P Lockledge
Organization: Tiptek, LLC
Title-Subject: [Filtered] Zeiss SEM Joystick Controller Lubricants
Message: Recently, we have experienced the joystick of a Zeiss SEM controller "sticking" and not releasing. The Zeiss service representative suggested trying a lubricant, but had no specific suggestions. I found this one online to be used successfully by gamers: Dow Corning Molykote 44 Medium Grease Lubricant.
Any experience with "sticking" joysticks, cleaning, or lubrication of them? Any suggestions would be appreciated.
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CeB6 is a little bit more expensive than a LaB6, this would be the only disadvantage I could think about… It is less sensitive to contamination than LaB6, yet you will still need a clean ultra high-vacuum in the gun (although not as low as a FEG). CeB6 has the advantage that it requires a lower evaporation temperature and it has a lower work function (2.7 eV for LaB6 vs 2.4 eV for CeB6). Therefore, you will get a longer lifetime of CeB6 over LaB6, and a slightly better brightness.
If you can afford a CeB6, go for it! You can also contact Mike Jercinovic at UMass Amherst (USA), he has more experience than I do with LaB6 vs CeB6 - I actually got all the information listed above from him when I was a postdoc at UMass Amherst :)
Julien
########################### *Dr. Julien Allaz *Head assistant for SEM/EPMA Inst. fĂĽr Geochemie und Petrologie ETH ZĂĽrich NWÂ E 84 Clausiusstrasse 25 8092Â ZĂĽrich Switzerland
} On 21 Feb 2019, at 22:51, microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America } To  Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } } } -------- Forwarded Message -------- } } Date: Thu, 21 Feb 2019 08:29:35 -0300 } X-from: Erico Freitas {ericotadeu-at-ufmg.br {mailto:ericotadeu-at-ufmg.br} } } } } } Dear all, } } } I would like to hear from the pros and cons of the cathode CeB6 in } comparison to the LaB6? } } } Kind regards, } } -- } Erico Freitas } } Physicist/Microscopist at Center of Microscopy } Universidade Federal de Minas Gerais (UFMG) } Av. Antônio Carlos, 6627, Campus Pampulha, Belo Horizonte, MG, Brazil. } ZIP Code 31270-901. } +55-31-3409-7573 } +55-31-3409-7575 } } Coordinator:Transmission Electron Microscopy laboratory } } CV Lattes: *http://lattes.cnpq.br/8786127123101199* } {https://wwws.cnpq.br/cvlattesweb/PKG_MENU.menu?f_cod=DE6B009EAB5F41052FDE9CDAAECDEB36#} } } ==============================Original Headers============================== } 16, 53 -- From microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} Thu Feb 21 } 15:46:33 2019 } 16, 53 -- Received: from mail-pf1-f170.google.com {http://mail-pf1-f170.google.com} } (mail-pf1-f170.google.com {http://mail-pf1-f170.google.com} [209.85.210.170]) } 16, 53 -- by microscopy.com {http://microscopy.com} (8.12.11.20060308/8.12.8) with ESMTP id } x1LLkXeW014366 } 16, 53 -- for {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } ; Thu, 21 Feb 2019 } 15:46:33 -0600 } 16, 53 -- Received: by mail-pf1-f170.google.com {http://mail-pf1-f170.google.com} with SMTP id } n74so60973pfi.9 } 16, 53 --         for {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } ; Thu, 21 Feb } 2019 13:55:19 -0800 (PST) } 16, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 16, 53 --         d=gmail.com {http://gmail.com} ; s=20161025; } 16, 53 --         h=subject:references:to:from:message-id:date:user-agent:mime-version } 16, 53 --          :in-reply-to:content-language:content-transfer-encoding; } 16, 53 --         bh=m3soXqomWYiGlGe/w7tiRh7k0U1akh33LHmqdDmNxCg=; } 16, 53 --         b=BtP6MWG0yf+2F3tTrPIXCZcLSLCN25Bi8/dBZW0lgP/h82k91mA3hHc/qs7etwRT3n } 16, 53 --          J8ypoRn9dshUEEdx9b/OZDytEubJ+6k8yYja6dYhD5y2sgRa95DUJD1ZJv4PndHMxvAT } 16, 53 --          ZFZEySrsH7HVO3dl7Y78EFtyVrAm9mf59L6DgG+0h9cwvflNN/q9zsJemZs/lc9tPtoq } 16, 53 --          UZ3NIKKZNCGIdXnIsq5v6Pe4fMl8YP+hChPSNNBRX/9BI6TWZ87DpFA5ZtOeQRXGnVr6 } 16, 53 --          H4ra2hRKhk5YlXRposwrd9oP7DTa0uVEjsD7o1rZ7Hc1pWrTKAsawM8fm7Tjz09iRJFS } 16, 53 --          1FxQ== } 16, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 16, 53 --         d=1e100.net {http://1e100.net} ; s=20161025; } 16, 53 --         h=x-gm-message-state:subject:references:to:from:message-id:date } 16, 53 --          :user-agent:mime-version:in-reply-to:content-language } 16, 53 --          :content-transfer-encoding; } 16, 53 --         bh=m3soXqomWYiGlGe/w7tiRh7k0U1akh33LHmqdDmNxCg=; } 16, 53 --         b=N7htXz+2lIw4+2deiKWLUNhd9e6el98AOMAJQZwKtODd4B2mR7WBlw/RVTVfNgAB2P } 16, 53 --          HUoJ+BCK5+MV/2yHrRpi98RTistV1R9iLKDSplOabomPqRoNCkIgvj/11hY+2Lkxxq+8 } 16, 53 --          RKG7mndjnIl6iFiZJjuOI46DIa3+OKNILeL0Z+tqJdLCsX6oD996rMkCv/YKv+U7xyk0 } 16, 53 --          qQQTGpwn1ATxSrlVnftrWMjUrfDEykrmZuwfWINTc9l9FrOQGs9cY1ULZC1+eqo2+aTM } 16, 53 --          Uos8d3aPOUxKqiM4XAIUK5SdbsOE5GEjES4HBc9bYA49PUPfwY1FvMmKnyAwIWdDfJq8 } 16, 53 --          +naA== } 16, 53 -- X-Gm-Message-State: AHQUAubvxi9la7rYkWR72rpjv1XSMnKhjAQyoM/GQFpT7/GGfCbUH13K } 16, 53 -- wZMrISobdJDVdsqOrQW3v5C8n7Ny } 16, 53 -- X-Google-Smtp-Source: } AHgI3IZh5lblYprsVdzH+Fnc2pLk9eYs3EvITm5tWCjvLJGclO6E2JB398cglb257ZKhkcTtybZGPA== } 16, 53 -- X-Received: by 2002:a63:5d48:: with SMTP id o8mr640224pgm.297.1550786118331; } 16, 53 --         Thu, 21 Feb 2019 13:55:18 -0800 (PST) } 16, 53 -- Received: from Nestor-MacBookAir-Pro-2014-ElCapitan.local ([137.154.167.142]) } 16, 53 --         by smtp.googlemail.com {http://smtp.googlemail.com} with ESMTPSA id } q62sm707pfi.183.2019.02.21.13.55.16 } 16, 53 --         for {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } } 16, 53 --         (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 16, 53 --         Thu, 21 Feb 2019 13:55:17 -0800 (PST) } 16, 53 -- Subject: Fwd: LaB6 or CeB6? 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Thanks for your reply. We have been used LaB6 emmiter in our TEM Tecnai ST20. We used Denka and Kimball. We had a look at the LaB6 and CeB6 specs in EMS website and found out they are pretty much they same, but CeB6 has a lower vapour pressure, and perhaps a lower work funcion, and it might have a longer life. But what puzzled us is that neither Kimball nor Denka produce CeB6, so we are wondering about its quality, though we would like to give it a try. But want to hear few more opinions before make our decision.
Regards,
On Thu, 21 Feb 2019, 19:10 Straszheim, Warren E [BIOTC], {wesaia-at-iastate.edu {mailto:wesaia-at-iastate.edu} } wrote:
I have not used one, but I think it would be quite comparable. I am used to LaB6 filaments in higher end research microscopes. I have only ever heard of CeB6 in desktop microscopes. CeB6 should be better than tungsten and maybe just a little worse than LaB6. If it is in a desktop microscope, I would be more concerned about the other design details. How is the rest of the column? How does it compare to a regular research microscope?
For our service lab, we run a field emitter on an FEI Quanta that is now rated at 1 nm resolution. We routinely push 100kx (based on a 5-inch Polaroid reference). If you based it on the image enlarged to the computer monitor, we would be pushing 300kx. How does that compare to your spec?
Warren Straszheim
-----Original Message----- From: microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} [mailto:microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} ] Sent: Thursday, February 21, 2019 3:47 PM To: Straszheim, Warren E [BIOTC] Subject: [Microscopy] Fwd: LaB6 or CeB6?
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Date:Â Â Thu, 21 Feb 2019 08:29:35 -0300 X-from:Â Â Â Â Â Erico Freitas {ericotadeu-at-ufmg.br {mailto:ericotadeu-at-ufmg.br} }
Dear all,
I would like to hear from the pros and cons of the cathode CeB6 in comparison to the LaB6?
Kind regards,
-- Erico Freitas
Physicist/Microscopist at Center of Microscopy Universidade Federal de Minas Gerais (UFMG) Av. Antônio Carlos, 6627, Campus Pampulha, Belo Horizonte, MG, Brazil. ZIP Code 31270-901. +55-31-3409-7573 +55-31-3409-7575
Coordinator:Transmission Electron Microscopy laboratory
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================================= Ludek Lovicar, PhD Manager of Electron Microscopy Facility Scientific Services Unit Institute of Science and Technology Austria Am Campus 1 3400 Klosterneuburg Austria
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Hope to see you there.
Aya Takase . Senior Scientist Rigaku Americas Corporation 9009 New Trails Drive . The Woodlands, TX 77381 USA T: 281-362-2300 ex 208 . F: 281-364-3628
==============================Original Headers============================== 11, 52 -- From btv1==963ad12df74==Aya.Takase-at-rigaku.com Fri Mar 1 11:31:08 2019 11, 52 -- Received: from spamfilter.rigaku.com (spamfilter.rigaku.com [146.20.167.112]) 11, 52 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x21HV7dc010189 11, 52 -- for {Microscopy-at-microscopy.com} ; Fri, 1 Mar 2019 11:31:08 -0600 11, 52 -- X-ASG-Debug-ID: 1551462018-0fdc517c23463dc0001-1DjkGe 11, 52 -- Received: from 592200-Exch2.rac.local ([172.16.230.5]) by spamfilter.rigaku.com with ESMTP id MNZ6IHCGWcr7MpXx (version=TLSv1.2 cipher=ECDHE-RSA-AES256-SHA384 bits=256 verify=NO) for {Microscopy-at-microscopy.com} ; Fri, 01 Mar 2019 12:40:18 -0500 (EST) 11, 52 -- X-Barracuda-Envelope-From: Aya.Takase-at-rigaku.com 11, 52 -- Content-Language: en-US 11, 52 -- Content-Type: text/plain; charset="iso-8859-1" 11, 52 -- DKIM-Signature: v=1; a=rsa-sha1; d=rigaku.com; s=dkim1; c=relaxed/relaxed; 11, 52 -- t=1551462018; h=from:subject:to:date:ad-hoc; 11, 52 -- bh=zY6deTj5IwsCIy+zhErPl8nH7lo=; 11, 52 -- b=diiXPzLYUnuVR7BBf1k+sSBzWMDiCXli+Qaotmj+7UlQs7JPU1Dli52DZabW3/haY4llDfzN5x9 11, 52 -- C4lYFsNN/92DAW2Ry5B3T2KpbPuK8851fHB4rX52Ns/Kcxt4CLB0H+1Vi8rHQzz3nQwQl8pQVMzjM 11, 52 -- l3/wHMHXzXRTRz3yir+RQhmvMlzUceM85hOrap45sqJRfmWOaqJwBEqgxBageFHJe8PSOI3jLysIs 11, 52 -- 9YIXo6MwuPPac3Z92D4DY4VjM4aLrq3wh66aO47dODY9ke7AwF67/wnB7vi8vCj2n1Yv41DI066eF 11, 52 -- sh6Gev0CxnYHo4F8eLDvPJZHznBwNuP1BNCg== 11, 52 -- Received: from 592194-EXCH1.rac.local (172.16.230.4) by 592200-Exch2.rac.local 11, 52 -- (172.16.230.5) with Microsoft SMTP Server (TLS) id 15.0.1365.1; Fri, 1 Mar 11, 52 -- 2019 12:40:18 -0500 11, 52 -- Received: from 592194-EXCH1.rac.local ([fe80::c40f:ace9:837:e2e]) by 11, 52 -- 592194-Exch1.rac.local ([fe80::c40f:ace9:837:e2e%18]) with mapi id 11, 52 -- 15.00.1365.000; Fri, 1 Mar 2019 12:40:18 -0500 11, 52 -- From: Aya Takase {Aya.Takase-at-rigaku.com} 11, 52 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 11, 52 -- Subject: Webinar series: X-ray Computed Tomography for Materials Science 11, 52 -- Thread-Topic: Webinar series: X-ray Computed Tomography for Materials Science 11, 52 -- X-ASG-Orig-Subj: Webinar series: X-ray Computed Tomography for Materials Science 11, 52 -- Thread-Index: AdTQVDZWunAMpyqsQbO0MXWmZ6t9/w== 11, 52 -- Date: Fri, 1 Mar 2019 17:40:17 +0000 11, 52 -- Message-ID: {ed0e9f4238c041c4947e5993a6cc2b4b-at-592194-Exch1.rac.local} 11, 52 -- Accept-Language: en-US 11, 52 -- X-MS-Has-Attach: 11, 52 -- X-MS-TNEF-Correlator: 11, 52 -- x-ms-exchange-transport-fromentityheader: Hosted 11, 52 -- x-originating-ip: [10.2.4.29] 11, 52 -- MIME-Version: 1.0 11, 52 -- X-Barracuda-Connect: UNKNOWN[172.16.230.5] 11, 52 -- X-Barracuda-Start-Time: 1551462018 11, 52 -- X-Barracuda-Encrypted: ECDHE-RSA-AES256-SHA384 11, 52 -- X-Barracuda-URL: https://spamfilter.rigaku.com:443/cgi-mod/mark.cgi 11, 52 -- X-Virus-Scanned: by bsmtpd at rigaku.com 11, 52 -- X-Barracuda-Scan-Msg-Size: 868 11, 52 -- X-Barracuda-BRTS-Status: 1 11, 52 -- X-Barracuda-Spam-Score: 0.00 11, 52 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using global scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=5.0 KILL_LEVEL=1000.0 tests= 11, 52 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.68052 11, 52 -- Rule breakdown below 11, 52 -- pts rule name description 11, 52 -- ---- ---------------------- -------------------------------------------------- 11, 52 -- Content-Transfer-Encoding: 8bit 11, 52 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id x21HV7dc010189 ==============================End of - Headers==============================
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Greetings,
The Monash Centre for Electron Microscopy (MCEM, see https://www.monash.edu/researchinfrastructure/mcem) is seeking to employ an Electron Microscopist to manage and develop MCEM's focussed ion beam capability and provide advanced expertise and training to support and enable the work of researchers using the centre.
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Email: tong.wang-at-asrc.cuny.edu Name: Tong Wang
Organization: ASRC at GC of CUNY
Title-Subject: [Filtered] Research Faculty - Nanoscience Initiative - Advanced Science Research Center in New York, New York
Message: Dear Colleagues, The Nanoscience Initiative (nanoscience.asrc.cuny.edu) at the CUNY Advanced Science Research Center (ASRC) invites applications for a full-time research faculty (open rank). Research Faculty hold full-time, non-tenure track positions and may serve as principal or co-principal investigators on grants or contracts, manage postdoctoral fellows and their research projects or supervise graduate or undergraduate student research. While they may participate in instructional programs, such as lectures or demonstrations, they are not assigned regular teaching duties. For further information and/or application please see:
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Message: We have large pieces of human brain samples taken during surgery that need to be processed for high contrast TEM. The remaining blood in the samples causes problems with the osmium and thiocarbohydrazide reactions.Does anyone know what can be used to "quench" the blood so it becomes less reactive. thanks in advance JoAnn
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Email: rvancamp-at-kettering.edu Name: R. Van Camp
Organization: Kettering University
Title-Subject: [Filtered] Questions: How to drying oils on Formvar
Message: I am currently working with a few fatty acids as a component of samples. I am finding oleic acid to be problematic and suspect I need to find a better way to dry the sample prior to placing it in the TEM.
Formvar coated grids are usually prepared with a layer or two of KimWipe behind them placed on top of my business card for additional moisture removal capacity, if necessary, and convenience in handling the grid. Samples are deposited onto each grid by dispensing a few drops from a disposable glass pipette (emphasize few). My practice is to place each grid in a desiccator cabinet for at least 24 hours before it is examined in our TEM.
This is materials science research performed at 200 kV in a JEM-2100 Plus using a W-hairpin filament. Beam current is ~15 uA.
I have observed sample vanish or, potentially grossly relocate, while attempting to focus on a specimen. As I decreased magnification to scan for a new site I observed dark rings surrounding the current specimen. I have never burned through a grid coating regardless of its composition so, I suspect the oleic acid is interacting with the electrons and enabling some currently unknown phenomena manifesting itself with the oleic acid or the acid/Formvar. Perhaps the heat deposited into the oil is warming or deteriorating the Formvar in some way.
Please let me know if you have suggestions for treating these oily grids before they are placed in the TEM.
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Thank you everyone for the suggestions to use sodium metaperiodate or hydrogen peroxide to quench the activity of blood cells during the processing. I am concerned that those chemicals may affect the quality of the ultrastructure, especially the sodium metaperiodate.
I am processing large blocks- greater than i.2 mm in thickness for connectomics study. I need to amplify the osmium staining and everything is done en bloc using THC to amplify the reduced osmium staining. This is followed by UA and lead acetate en bloc. We are not doing any post staining or immuno. Perhaps a low concentration of sodium peroxide will do the trick. I can try it on 1 or 2 of the slices and we can try to target the areas that have the clots.
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Title-Subject: [Filtered] Looking for Application specialist
Message: Hello, TESCAN USA is looking for FIB/SEM application specialist. If anybody is interested, please fill free to contact ldumas-at-tescan-usa.com. Thanks
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The Center for Advanced Materials Characterization in Oregon (CAMCOR) at the University of Oregon (UO) is looking for a new FIB-SEM Lab Manager. The Focused Ion Beam-Scanning Electron Microscopy (FIB-SEM) Lab Manager is involved in all activity within the FIB-SEM facility at the Center for Advanced Material Characterization in Oregon (CAMCOR). This includes providing professional technical management and services to support shared equipment in the CAMCOR laboratories; user training and technical support on SEM, FIB-SEM, and related equipment; overseeing instrument upgrades and maintenance; assisting with course development and teaching of laboratory components; providing analytical services for internal and external clients; and maintaining and conducting facility tours and demonstrations.
Review of applications begins on April 1, 2019 and will remain open until the position is filled. More information about required and preferred qualifications and working at UO can be found at: http://careers.uoregon.edu/cw/en-us/job/523598/fibsem-lab-manager
-Julie
Julie Chouinard CAMCOR Surface Analytical and Microanalytical Facilities 1241 University of Oregon Eugene, OR 97403 jbarkman-at-uoregon.edu (541)-346-4580
==============================Original Headers============================== 5, 46 -- From jbarkman-at-uoregon.edu Mon Mar 11 10:44:45 2019 5, 46 -- Received: from mx0a-000bfd01.pphosted.com (mx0a-000bfd01.pphosted.com [148.163.145.154]) 5, 46 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x2BFijEF016420 5, 46 -- for {Microscopy-at-microscopy.com} ; Mon, 11 Mar 2019 10:44:45 -0500 5, 46 -- Received: from pps.filterd (m0158912.ppops.net [127.0.0.1]) 5, 46 -- by mx0a-000bfd01.pphosted.com (8.16.0.27/8.16.0.27) with SMTP id x2BFjHax030556 5, 46 -- for {Microscopy-at-microscopy.com} ; Mon, 11 Mar 2019 15:54:29 GMT 5, 46 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=uoregon.edu; h=mime-version : 5, 46 -- content-type : content-transfer-encoding : date : from : to : subject : 5, 46 -- message-id; s=uoregon.edu; 5, 46 -- bh=f35Qd3TVKtRKEwWEWh83352uNNgrkoHA7acCbNWjTx0=; 5, 46 -- b=WzN7WsPEKAs1O70ZuCRfeUZCnEIxg6/h/6jLLa6HZkasfkETNvL0sE8pncAt4CBSB0W5 5, 46 -- e7b2JV7VwN1hXckPiKOrEl4FGGM+LuvtUasdLqsc7hlT9u7YrQjGQtbT7Y0P4D00e187 5, 46 -- b5R7ymH/sjPYpSa7YJw9xxES/WdItpIujo7HT/Gk7C9A0bL6TLV9YDkDHqzQYlwX8rPl 5, 46 -- 4QkdtkXErQmTys/vIE7HyHO9KpOFpS3R9vhjfdol5677/zYjlilzt77m591i07HFOyiC 5, 46 -- 0SFISeD2B+T+LHbgNN/mWN0RQok7EeDATfpwnX2e1GA7xzJJR4b8ougaY0q/Ju92TvjQ iw== 5, 46 -- Received: from smtp.uoregon.edu (cc-smtp2.uoregon.edu [184.171.108.230]) 5, 46 -- by mx0a-000bfd01.pphosted.com with ESMTP id 2r4512mert-1 5, 46 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES256-GCM-SHA384 bits=256 verify=NOT) 5, 46 -- for {Microscopy-at-microscopy.com} ; Mon, 11 Mar 2019 15:54:29 +0000 5, 46 -- Received: from webmail.uoregon.edu (webmail1.uoregon.edu [128.223.143.235] (may be forged)) 5, 46 -- (authenticated bits=0) 5, 46 -- by smtp.uoregon.edu (8.14.4/8.14.4) with ESMTP id x2BFsS88031528 5, 46 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-GCM-SHA384 bits=256 verify=NOT) 5, 46 -- for {Microscopy-at-microscopy.com} ; Mon, 11 Mar 2019 08:54:28 -0700 5, 46 -- Received: from 2607:8400:20c6:2:69d6:96cb:d6ef:806e 5, 46 -- by webmail.uoregon.edu 5, 46 -- with HTTP (HTTP/1.1 POST); Mon, 11 Mar 2019 08:54:28 -0700 5, 46 -- MIME-Version: 1.0 5, 46 -- Content-Type: text/plain; charset=US-ASCII; 5, 46 -- format=flowed 5, 46 -- Content-Transfer-Encoding: 7bit 5, 46 -- Date: Mon, 11 Mar 2019 08:54:28 -0700 5, 46 -- From: Julie Chouinard {jbarkman-at-uoregon.edu} 5, 46 -- To: Microscopy-at-microscopy.com 5, 46 -- Subject: FIB-SEM Lab Manager Wanted 5, 46 -- Message-ID: {dcd025d496505729f7658a3743c13b2a-at-uoregon.edu} 5, 46 -- X-Sender: jbarkman-at-uoregon.edu 5, 46 -- User-Agent: Roundcube Webmail/1.1.10 5, 46 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10434:,, definitions=2019-03-11_12:,, 5, 46 -- signatures=0 5, 46 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 priorityscore=1501 malwarescore=0 5, 46 -- suspectscore=1 phishscore=0 bulkscore=0 spamscore=0 clxscore=1015 5, 46 -- lowpriorityscore=0 mlxscore=0 impostorscore=0 mlxlogscore=957 adultscore=0 5, 46 -- classifier=spam adjust=-40 reason=mlx scancount=1 engine=8.0.1-1810050000 5, 46 -- definitions=main-1903110113 ==============================End of - Headers==============================
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Email: ralvaradojr-at-ufl.edu Name: Rudy
Organization: University of Florida Title-Subject: [Filtered] LAMP1 antibody on HM20 resin
Message: Has anyone tried immunoEM labeling using LAMP1 antibody on HM20 resin? If you were successful, can you please send me information from that antibody (e.g., brand, type, etc.). Thank you
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Message: Dear Fellow Microscopists: This is to inform you that Southern California Society for Microscopy and Microanalysis (SCSMM) 2019 spring symposium is scheduled for Friday, April 12, 2019 at Cali2 Auditorium, UC Irvine campus. In the past decade microscopy and spectroscopy made major advancements reflected in few recent Nobel Prizes in Chemistry: in 2008 for the discovery and development of the green fluorescent protein; 2014 for the development of super-resolved fluorescence microscopy; 2017 for the cryo electron microscopy and 2018 for the directed evolution of enzymes. We are proud that among contributors to those great achievements are representatives of California: Roger Y. Tsien (2008), Howard Hughes Medical Institute, University of California, San Diego, La Jolla; William E. Moerner (2014) Stanford University, Stanford, CA; Frances H. Arnold (2018) California Institute of Technology, Pasadena. We would like to mark those achievements and are pleased that in our spring symposium we have contribution of the leading hubs representing life sciences in California. We are proud to have Bruce Cohen from Lawrence Berkeley National Laboratory (LBNL, Berkeley) as our 2019 MSA tour speaker. We also have Daniela Boassa from National Center for Microscopy and Imaging Research (NCMIR, San Diego) and Mark Herzik from UCSD (San Diego). Please register no later than 5 p.m. Friday, April 5th: https://imri.uci.edu/content/2019-spring-meeting-registration#overlay-context Check the “Next Meeting” updates on the website (www.scsmm.org). Annual regular membership is $25 (student $10) and this includes our Spring symposium and fall meeting. On-line registration is required. At the symposium we will have both student platform talks and poster presentations. Please send us your abstracts by March 15, 2019. Students are encouraged to contribute with presentation at the SCSMM meeting. You'll get the chance 1) to win up to $500; 2) become a member of professional society; 3) build up your network; 4) get your presentation at scientific meeting. This year we continue to hold the SCSMM Image Contest. Please send us your images by April 6, 2019. We also have a few prizes for the image contest. For more details, please see SCSMM website (www.scsmm.org) and also follow SCSMM Facebook page. We look forward to seeing you all at the symposium. Sincerely, SCSMM board www.scsmm.org https://www.facebook.com/microscopymicroanalysis
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We pleased to announce that registration is now open for the second annual Next-Generation Transmission Electron Microscopy (NexTEM) workshop, which will be held as a pre-meeting congress for Microscopy and Microanalysis 2019. This event will bring together researchers from diverse backgrounds to present the state-of-the-art in cutting edge electron microscopy tools and related applications. We are excited to again feature distinguished speakers from across the microscopy field.
Specific areas of interest include advanced detector designs for all states of matter, recent developments in electron and phonon optics/instrumentation, in situ and ultrafast TEM, cryo TEM, as well as data analytics and computational methods. Stimulating discussion and presentation of electron microscopy tools at the intersection of these fields will set the stage for advancement in these areas, as well as collaborative approaches to critical scientific issues in materials science, biology and medicine, physics, and chemistry.
Please visit the following link to register: https://www.microscopy.org/MandM/2019/program/congress.cfm
Workshop Topics Include:
Advanced Detector and Spectroscopy Developments * Design and use of novel detectors to investigate material structure and functionality, including 4D STEM and ptychography. * Vibrational and phonon spectroscopies at unprecedented spatial and energy resolution. * Methods to conduct high-resolution imaging and spectroscopy of beam-sensitive samples. * Examination of materials structure and chemistry at cryogenic temperatures.
Frontiers of In Situ / Operando Microscopy * Advances in S/TEM methods and instrumentation to capture the dynamics of complex materials systems, including alloys, thin films, nanoparticles, and liquids. * Investigation of materials under stimulus across a range of sample environments and temperatures. * New workflows for in situ experimentation to ensure reliability, reproducibility, and improve data quality.
Data-Driven Microscopy and Analysis * Machine learning-based analysis of materials structure, dynamics, and defects. * Integration of multiple large-scale imaging and spectroscopic data streams to elucidate physical descriptors of complex systems and phenomena. * High-throughput simulation approaches to guide the interpretation of experimental datasets.
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Email: ycding-at-g.ucla.edu Name: Yichen
Organization: UCLA
Title-Subject: [Filtered] Postdoc position
Message: We are now recruiting one postdoc who is interested in optical microscopy, especially light-sheet fluorescence imaging in the Department of Medicine at UCLA. The potential candidate will be responsible for system construction, system control, image analysis and biomedical applications.
For more details, please send your CV and a brief introduction to your background to Tzung (THsiai-at-mednet.ucla.edu) or Yichen (ycding-at-g.ucla.edu). Thank you! Login Host: 149.142.103.215 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
From buviregi380boha-at-gmail.com Tue Mar 12 19:53:55 2019 Return-Path: {buviregi380boha-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.161.194] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x2D0rrC7004017 for {microscopylistserverarchive7-at-microscopy.com} ; Tue, 12 Mar 2019 19:53:54 -0500 Received: from unknown (HELO mx.reskind.net) (Wed, 13 Mar 2019 12:46:28 +1200) by smtp.doneohx.com with SMTP; Wed, 13 Mar 2019 12:46:28 +1200 Received: from mxs.perenter.com ([72.36.87.107]) by mx03.listsystemsf.net with SMTP; Wed, 13 Mar 2019 12:33:47 +1200 Received: from smtp.mixedthings.net ([Wed, 13 Mar 2019 12:28:40 +1200]) by mail.naihautsui.co.kr with NNFMP; Wed, 13 Mar 2019 12:28:40 +1200 Received: from [195.33.250.117] by smtp.endend.nl with SMTP; Wed, 13 Mar 2019 12:11:26 +1200 Message-ID: {0D7FC94B.C9273E0D-at-gmail.com}
NIST PML (Physical Measurements Laboratory, Nanoscale Imaging Group) has an active research program on development of in situ scanning electron microscopy characterization and microfabrication of objects and devices in liquids and dense gaseous environments using environmental (micro-) fluidic (flow) cells equipped with electron transparent windows (including graphene). We have excellent experimental opportunities to conduct this research line including an array of dedicated SEM microscopes: JEOL VP 7800 (equipped with: TTL SE, SED, BSE, transmitted electrons, ions detector, EDX, EBIC/EBAC, X-ray microtomography, cooling/ heating stages, flow cells etc), UHV Zeiss SEM (part of Omicron multiprobe UHV system) coupled with AES , XPS, FIB capabilities, Zeiss EVO coupled with micro-Raman spectrometer), clean room micro-fabrication facilities, in-lab sample preparation facilities (Leica 600 sputter/evaporator, AJA sputter/evaporator, UV/plasma cleaning/ashing and etc.
Currently, a postdoctoral position for highly motivated and experienced experimentalist is available.
US citizens are welcomed to explore the related NRC postdoctoral fellowship opportunity: https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fnrc58.nas.edu%2FRAPLab10%2FOpportunity%2FOpportunity.aspx%3FLabCode%3D50%26ROPCD%3D506201%26RONum%3DB8512&data=02%7C01%7Candrei.kolmakov%40nist.gov%7C94654693292e41d2733208d6905af30e%7C2ab5d82fd8fa4797a93e054655c61dec%7C1%7C0%7C636855117643524885&sdata=PFtVSmxf%2BzSAGK%2B7hsz72bRhZznnSePJLqO83VWeEgI%3D&reserved=0
The preferable set of skills includes but not limited to: liquid /atmospheric pressure SEM/TEM, electrochemistry, EBIC, XPS, AES, AFM, UHV surface science protocols, clean room experience, excellent writing and teamwork skills. The letter of interest, CV, with a publication record and contact names of 3-4 references should be sent to Dr. Andrei Kolmakov, andrei.kolmakov-at-nist.gov
Thank you for your interest _______________________________ Andrei Kolmakov Ph.D. Project Leader, Nanoscale Imaging Group Nanoscale Device Characterization Div. Physical Measurements Laboratory,
NIST 100 Bureau Drive Gaithersburg, MD 20899 MS 6204
==============================Original Headers============================== 12, 60 -- From andrei.kolmakov-at-nist.gov Wed Mar 13 09:21:25 2019 12, 60 -- Received: from GCC01-CY1-obe.outbound.protection.outlook.com (mail-eopbgr830124.outbound.protection.outlook.com [40.107.83.124]) 12, 60 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x2DELOrR005328 12, 60 -- for {Microscopy-at-microscopy.com} ; Wed, 13 Mar 2019 09:21:25 -0500 12, 60 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=nist.gov; s=selector1; 12, 60 -- h=From:Date:Subject:Message-ID:Content-Type:MIME-Version:X-MS-Exchange-SenderADCheck; 12, 60 -- bh=OqEX7HRdPzKMhwFRtFlN8kUp1HAY57r9jfM0tM5wDrw=; 12, 60 -- b=Q67aI+D/xV+j+zYagseEoRlEc4fdBCfaKv+9avcQqDKdzEqi5kVNlQKqt57FgTy2rOZh3uPCZpW6477rnc9neCBuJkKwYo8UaW06flzmyo+k3JL7oM4EweSsb3LVpzj/kePXiiop71vSLK5I7PUbWFGbYlqwCT9hdYhUVYb/29w= 12, 60 -- Received: from DM2PR09MB0590.namprd09.prod.outlook.com (10.161.252.24) by 12, 60 -- DM2PR09MB0591.namprd09.prod.outlook.com (10.161.252.25) with Microsoft SMTP 12, 60 -- Server (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_256_GCM_SHA384) id 12, 60 -- 15.20.1709.13; Wed, 13 Mar 2019 14:31:13 +0000 12, 60 -- Received: from DM2PR09MB0590.namprd09.prod.outlook.com 12, 60 -- ([fe80::8892:8a04:fa1:373f]) by DM2PR09MB0590.namprd09.prod.outlook.com 12, 60 -- ([fe80::8892:8a04:fa1:373f%4]) with mapi id 15.20.1686.021; Wed, 13 Mar 2019 12, 60 -- 14:31:13 +0000 12, 60 -- From: "Kolmakov, Andrei A. (Fed)" {andrei.kolmakov-at-nist.gov} 12, 60 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 12, 60 -- Subject: in situ electron microscopy postdoc at NIST 12, 60 -- Thread-Topic: in situ electron microscopy postdoc at NIST 12, 60 -- Thread-Index: AdTZqRGOlf0LdYzCRx6uK5PC/oWpjA== 12, 60 -- Date: Wed, 13 Mar 2019 14:31:13 +0000 12, 60 -- Message-ID: 12, 60 -- {DM2PR09MB059054A3C2AD56B285836C2B874A0-at-DM2PR09MB0590.namprd09.prod.outlook.com} 12, 60 -- Accept-Language: en-US 12, 60 -- Content-Language: en-US 12, 60 -- X-MS-Has-Attach: 12, 60 -- X-MS-TNEF-Correlator: 12, 60 -- authentication-results: spf=none (sender IP is ) 12, 60 -- smtp.mailfrom=andrei.kolmakov-at-nist.gov; 12, 60 -- x-originating-ip: [129.6.135.102] 12, 60 -- x-ms-publictraffictype: Email 12, 60 -- x-ms-office365-filtering-correlation-id: 89cebcf7-cc3f-40da-1555-08d6a7c08b7a 12, 60 -- x-ms-office365-filtering-ht: Tenant 12, 60 -- x-microsoft-antispam: 12, 60 -- BCL:0;PCL:0;RULEID:(2390118)(7020095)(4652040)(8989299)(4534185)(4627221)(201703031133081)(201702281549075)(8990200)(5600127)(711020)(4605104)(4618075)(2017052603328)(7153060)(7193020);SRVR:DM2PR09MB0591; 12, 60 -- x-ms-traffictypediagnostic: DM2PR09MB0591: 12, 60 -- x-ms-exchange-purlcount: 2 12, 60 -- x-microsoft-antispam-prvs: 12, 60 -- {DM2PR09MB0591803773786ABA77C3C822874A0-at-DM2PR09MB0591.namprd09.prod.outlook.com} 12, 60 -- x-forefront-prvs: 09752BC779 12, 60 -- x-forefront-antispam-report: 12, 60 -- SFV:NSPM;SFS:(10019020)(366004)(346002)(136003)(376002)(396003)(39860400002)(199004)(189003)(8936002)(966005)(486006)(476003)(478600001)(14454004)(86362001)(33656002)(2906002)(6116002)(3846002)(316002)(68736007)(4743002)(53936002)(25786009)(6436002)(305945005)(7736002)(5640700003)(6306002)(74316002)(9686003)(6916009)(7696005)(66066001)(81156014)(26005)(8676002)(6506007)(102836004)(186003)(81166006)(52536013)(99286004)(71190400001)(71200400001)(5660300002)(45080400002)(105586002)(106356001)(256004)(2351001)(97736004)(55016002)(2501003);DIR:OUT;SFP:1102;SCL:1;SRVR:DM2PR09MB0591;H:DM2PR09MB0590.namprd09.prod.outlook.com;FPR:;SPF:None;LANG:en;PTR:InfoNoRecords;A:1;MX:1; 12, 60 -- received-spf: None (protection.outlook.com: nist.gov does not designate 12, 60 -- permitted sender hosts) 12, 60 -- x-ms-exchange-senderadcheck: 1 12, 60 -- x-microsoft-antispam-message-info: 12, 60 -- OkovQaQboY6G/nO2f3hzNLgGgzZLMxH5LvVXA8Oema12Vk94/xsF8X8yPxiC3Qe1tjiPppnybj+Cc0cm7829z45c8wy7DTW++iaaHYIEf9y0oxBmAnRnlnvR7Fb6hj6SCD679j9NEbhnS1fykG7eYFqCFU35ZTYJ9k4MfVkKGZVsPexuKEgTjCDno5q1zkq+pUPoUIhBTC6V2B7hul1qBuXhtKjaufL7X5MG/gipqcMpLi++m+F5l9tZmz+ZTUoDlIQAte2GUAhdQ7iL7G2aBf1OVordCDSEfYVvd8bsUkIw5FtpRshnGuwS2AvitfYSEMSKbogbHbnIDIV7z+MenQEc3ymQaDqxP5t0OfD5v3YpdAN42nBDGrvY2n3J10pRANb3e/hvh2bcVmJzk/4xlHWdS0YWYM0B4o9JmWz0hyg= 12, 60 -- Content-Type: text/plain; charset="us-ascii" 12, 60 -- MIME-Version: 1.0 12, 60 -- X-OriginatorOrg: nist.gov 12, 60 -- X-MS-Exchange-CrossTenant-Network-Message-Id: 89cebcf7-cc3f-40da-1555-08d6a7c08b7a 12, 60 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 13 Mar 2019 14:31:13.3676 12, 60 -- (UTC) 12, 60 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted 12, 60 -- X-MS-Exchange-CrossTenant-id: 2ab5d82f-d8fa-4797-a93e-054655c61dec 12, 60 -- X-MS-Exchange-CrossTenant-mailboxtype: HOSTED 12, 60 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: DM2PR09MB0591 12, 60 -- Content-Transfer-Encoding: 8bit 12, 60 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id x2DELOrR005328 ==============================End of - Headers==============================
From tammyhoward072mfiec-at-gmail.com Fri Mar 15 05:11:59 2019 Return-Path: {tammyhoward072mfiec-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.161.202] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x2FABuBJ013818 for {microscopylistserverarchive7-at-microscopy.com} ; Fri, 15 Mar 2019 05:11:58 -0500 Received: from relay-x.misswldrs.com ([Fri, 15 Mar 2019 07:13:44 -0300]) by qnx.mdrost.com with SMTP; Fri, 15 Mar 2019 07:13:44 -0300 Message-ID: {E442FAF2.8D784469-at-gmail.com}
-------- Forwarded Message --------
X-from: whiteto-at-missouri.edu
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Email: whiteto-at-missouri.edu Name: Tommi White
Organization: University of Missouri Electron Microscopy Core
Title-Subject: [Filtered] Structural Electron Microsocpy: Missouri Symposium for Molecular Biophysics
Message: Dear Microscopy ListServer members
Please join us for the 3rd Missouri Symposium in Molecular Biophysics with a focus on "Structural Electron Microscopy" on April 25-26th, 2019 at the University of Missouri?s Bond Life Sciences Center. University of Missouri System anticipates building our facilities and efforts in the near future in structural electron microscopy. http://emc.missouri.edu/missourisymposium/
The Missouri Symposium will host many leaders in structural biology and electron microscopy. This symposium is targeted both towards the life and material scientist to educate them on the possibilities utilizing future electron microscopy investments and how they can further your research interests. On Thursday April 25th we will have a welcome dinner, participant poster session and keynote address from Dr. Wah Chiu from the SLAC-Stanford CryoEM Center and Friday April 26th will include a day filled with seminars from leaders in this emerging field. Please register in advance ($100/non-student; $50/student) and see our website for more details. We hope you can join us! Below is a very brief summary of the speakers and a representative DOI-link of their work. Please reach out to me with any questions.
Tommi Tommi A. White, Ph.D. Assistant Professor of Biochemistry Director, Electron Microscopy Core Facility University of Missouri W117 Veterinary Medicine Building 1600 East Rollins Street Columbia, MO 65211 573-882-8304 WhiteTo-at-missouri.edu http://emc.missouri.edu
~~~~~~~~~~~~~ Wah Chiu (KEYNOTE) - For over 3 decades Dr. Chiu has lead methodology development for electron cryo-microscopy. His work has made multiple transformational contributions in developing single particle electron cryo-microscopy as a tool for the structural determination of molecular machines towards atomic resolution (DOI:10.1016/j.molcel.2018.02.006).
Elizabeth Wright - has developed novel methods to perform correlative light and electron microscopy at cryogenic temperatures, preserving ultrastructural details to visualize events occurring during viral infection at the micro- and nanoscales, with light and electrons respectively (DOI:10.1017/S1431927618012382).
Todd Yeates - The structure of a protein much smaller than 50 kDa can be successfully visualized when it is attached to a large protein scaffold designed to hold 12 copies of the attached protein in symmetric and rigidly defined orientation (DOI:10.1073/pnas.1718825115).
Tamir Gonen - pioneered the field of "Micro Electron Diffraction" that is garnering much interest from numerous fields for quick high resolution structure determination of crystalline small molecules as well as crystalline proteins (DOI:10.1021/acscentsci.8b00760).
Lena Kourkoutis - develops and applies novel electron microscopy techniques to advance the fundamental understanding of materials and devices, extending the reach of aberration corrected STEM to cryogenic temperature. Operating at low temperatures provides access to a broad range of electronic phases that emerge during cooling of quantum materials and also allows the study of processes that occur at liquid-solid interfaces (DOI:10.1038/s41586-018-0397-3).
Phoebe Stewart - using cryoEM to characterize targeted gene therapy and other nanoscale treatments including viruses, viral/host factor complexes, engineered adenovirus-based vaccines, DNA double-strand break repair complexes, and circadian clock protein complexes (DOI:10.1039/c6nr06948g).
Michael Stowell - studies how neurons communicate and their subsequent alteration upon disease using structural methods (X-ray crystallography, cryoEM and tomography). Recently, he has determined the structure of an ion transporter (DOI:10.1101/505453)
Jeffrey Lengyel - Lead Principal Scientist for Thermofisher Scientific (formerly FEI Company) educating his customers on their microscopy tools and other related cutting-edge technologies (DOI:10.1007/s10969-014-9179-9).
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Dear All: I was wondering if any of you could help with an oft reoccurring problem we have in our facility. We are unfortunate to be in a building where the heating is not well controlled in winter. The temperature our microscopes are in can vary from 60 - 80++ °F (well roughly). Ideally, we would like them to be at a temperature of around 74 °F, and for that temperature to be as constant as possible. We have managed this in the past by running window AC's but this is not a good solution as the temperature outside can be below freezing, and normal AC's don't work well in those conditions. Opening a window or using a fan is not a good solution as we want the temperature to be stable.
I have looked at all season AC units, but these just seem to be regular ACs with heaters in them, they don't address the problem of how to keep the room cool when the building as a whole is hot and the outside weather is cold.
Suggestions.
Thanks Lloyd Williams
Director Bio-Imaging & Network Core Facilities Hunter College, City University of New York Department of Biological Sciences Rm. 826 HN 695 Park Ave New York, NY 10065 212 650 3872; fax: 212 650-3656
Director Bio-Imaging & Network Core Facilities Hunter College, City University of New York Department of Biological Sciences Rm. 826 HN 695 Park Ave New York, NY 10065 212 650 3872; fax: 212 650-3656
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Department of Physics The University of Illinois at Chicago
845 W Taylor Street, M/C 273 Chicago, IL 60607 Tel: (312) 996-6064 Fax: (312) 996-9016
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Quantitative Microanalysis 2019 will be held at the University of Minnesota, Minneapolis June 24-27th. This four day MAS Topical Conference consists of user group meetings followed by a three day plenary meeting covering quantitative microanalysis for beginners to experts, and focusing on EPMA, SEM, and advances in microanalysis. The conference includes tutorials, invited and contributed presentations, laboratory demos, and group discussion.
Our invited speakers to date include: Ben Buse (University of Bristol, UK): Overview of EPMA-WDS and field emission gun EPMA. John Donovan (Probe Software, USA): Compositional mapping. Karsten Goemann (University of Tasmania, Hobart, Australia): Fine tuning SEM for quantitative analysis and combined EDS-WDS Mike Jercinovic (University of Massachusetts, Amherst, USA), EPMA trace element analysis Colin MacRae (CSIRO Mineral Resources, Clayton, Australia): Cathodoluminescence and hydrated mineral analyses William Nachlas (Syracuse University, USA): Standards and reference materials
Early Career Scholar student financial support deadline has been extended to April 1. Reimbursement will be prioritized for those students and early career professionals who submit an abstract for a platform or poster presentation prior to April 1 followed by those students attending the topical conference.
Early bird registration for professionals ends March 31. Register before rates go up!
Look forward to seeing you at QMA 2019!
The QMA 2019 committee
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X-from: Beavers, Roy {rbeavers-at-mail.smu.edu}
Looking for good companies that deal in used petrographic microscopes.
Prefer Nikon or Zeiss scopes that can accommodate high resolution cameras.
I'm working with a sample that is really sensitive to temperatures above room temperature.
I need to polish the sample to make a wedge sample for TEM imaging. I need to glue the sample onto a polisher holder, however i need to find a M-bond, wax, or glue that can be cured at room temperature and will be hard enough so the sample stays in the holder while polishing! Also, easy to be removed with a simple solvent.
The sample is a InSb (semiconductor) substrate. Really brittle and soft as well.
Please let me know if you know of any product and thanks in advance.
Rosa
/─────────//─────────//─────────//───────//─/ /Rosa E. Diaz, PhD/ /Electron Microscopy Research Scientist / /Birck Nanotechnology Center/ /Purdue University/ /1205 W. State Street, West Lafayette, IN 47907-2057 / /Office: BRK-1272  Tel ://765-496-1075/ /─────────//─────────//─────────//───────//─/
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Email: becks-at-ncc.edu Name: Stephen Beck
Organization: Nassau Community College
Title-Subject: [Filtered] Critical Point Dryer Problem
Message: Dear Colleagues,
I have just received a Tousimis 931 critical point dryer to replace a vintage 1992 Polaron Jumbo CPD. I am having problems with residual ethanol in the chamber (large 3.5” diameter) after the completion of the critical point process. Samples are therefore wet and subject to surface tension distortion/collapse. Has anyone else had a similar problem? I have been working with the company tech support and have verified my liquid CO2 tank volume and syphon tube, increased the AUTO purge time from 10 to 15 minutes and verified the metering valve factory settings. Any suggestions are appreciated!
Steve
Stephen J. Beck Professor Coordinator, Bio-Imaging Center Electron Microscopy Department of Biology Nassau Community College Garden City, NY 11530
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I've had good luck using Loctite 460 super glue. It cures at room temperature in seconds or minutes and can be removed by soaking sample and stub in acetone. Shear strength should be sufficient for tripod/wedge polishing; if not, there is a Loctite 454 super glue that's a bit stronger in shear (from what I understand).
Good luck with your InSb sample. That's not a fun material system to polish.
Cheers, Chris
On Tue, Mar 19, 2019 at 8:49 PM {microscopy.listserver-at-gmail.com} wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } } } -------- Forwarded Message -------- } } } X-from: Rosa Diaz {rdiazri-at-purdue.edu} } } } } } Hello, } } I'm working with a sample that is really sensitive to temperatures above room temperature. } } I need to polish the sample to make a wedge sample for TEM imaging. I need to glue the sample onto a } polisher holder, however i need to find a M-bond, wax, or glue that can be cured at room temperature } and will be hard enough so the sample stays in the holder while polishing! Also, easy to be removed } with a simple solvent. } } The sample is a InSb (semiconductor) substrate. Really brittle and soft as well. } } } Please let me know if you know of any product and thanks in advance. } } Rosa } } /─────────//─────────//─────────//───────//─/ } /Rosa E. Diaz, PhD/ } /Electron Microscopy Research Scientist / } /Birck Nanotechnology Center/ } /Purdue University/ } /1205 W. State Street, West Lafayette, IN 47907-2057 / } /Office: BRK-1272 Tel ://765-496-1075/ } /─────────//─────────//─────────//───────//─/ } } ==============================Original Headers============================== } 17, 54 -- From microscopy.listserver-at-gmail.com Tue Mar 19 19:37:37 2019 } 17, 54 -- Received: from mail-io1-f51.google.com (mail-io1-f51.google.com [209.85.166.51]) } 17, 54 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x2K0bbio031373 } 17, 54 -- for {microscopy-at-microscopy.com} ; Tue, 19 Mar 2019 19:37:37 -0500 } 17, 54 -- Received: by mail-io1-f51.google.com with SMTP id x7so446941ioh.4 } 17, 54 -- for {microscopy-at-microscopy.com} ; Tue, 19 Mar 2019 17:47:49 -0700 (PDT) } 17, 54 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 17, 54 -- d=gmail.com; s=20161025; } 17, 54 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 17, 54 -- :in-reply-to:content-language:content-transfer-encoding; } 17, 54 -- bh=0tausKRgqohi8nbYstZQ2CU727olIfkNh20AHnkaO5E=; } 17, 54 -- b=CAPwMLPNLrUdiAxVJPYiHeBQv4VW7eteK5NTTVxqQIFZj4hv2lX593fL+V4GtctAbM } 17, 54 -- wudNJ1PNU60Cbu1LIPuegkUXr39t0HgJhFIjzJixuN+xbEHJAyPZWvWN/G7TDo9/Z+Ac } 17, 54 -- u+5R+tUjBf5FSbWeb5xQmlwExI077wlHZd7lXqSXibhJiY0nVFB0mh/l6S83pWk5H7pS } 17, 54 -- A2V7Viix6mXt2SQZFg0F3C9ipAZUMIFrsQKkrVyfyvTt3gtEpfQo8I4hq38TTq/2ZxBO } 17, 54 -- mJXmvrr71RQsUw3qE5ZJ2d5jv7X2OHmr7UV7XP+SA/qzmBpy91noqlvn2EfxaWsJVHg0 } 17, 54 -- imbg== } 17, 54 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 17, 54 -- d=1e100.net; s=20161025; } 17, 54 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 17, 54 -- :user-agent:mime-version:in-reply-to:content-language } 17, 54 -- :content-transfer-encoding; } 17, 54 -- bh=0tausKRgqohi8nbYstZQ2CU727olIfkNh20AHnkaO5E=; } 17, 54 -- b=FV73M5qi+fDAotcK81J/NiqUft65SRhY9osZ0+ob6aJbnv2H27YgwhipJk5rDrGkfB } 17, 54 -- gf/7puUpsE9nDUUzaUVxIVb0VfLEfrogUKP2ExlQzrMrqoBFYTrdlxqvxXqX1rUH2gV5 } 17, 54 -- k0jM6BwiLEU2KXjU41Ou46L4iCZj15yRlNm4z+4fPZK2YSaJMthBTDfeqJXCPZyFZjn3 } 17, 54 -- oXYZfKYz7MJbSt4yJO9T5OJaWngEZxTVr1DpRcvsU7gWz3nYFZNbNFmLSgENUzTkQkOT } 17, 54 -- MWxzwRlMsXodPTX79xiondekkWIVGzVWQPCXEQqp7IQ9Ks8JEkP/dWCVL8o7MareCbo1 } 17, 54 -- BpuQ== } 17, 54 -- X-Gm-Message-State: APjAAAVqOd+HbRo86wnkPbGvpzqP0+2q954n76UwV19fcwkFDDykk1OX } 17, 54 -- KRYhr32wdcsiILCE5c3Kqj/5XNgY } 17, 54 -- X-Google-Smtp-Source: APXvYqx4/gQu3rI1HshczdKuHzIXepkz7bBxBxShoUu7LX+aJJdbBy8404PKStOTKEC/pZAWg9Ta5A== } 17, 54 -- X-Received: by 2002:a6b:4e10:: with SMTP id c16mr3306167iob.18.1553042868516; } 17, 54 -- Tue, 19 Mar 2019 17:47:48 -0700 (PDT) } 17, 54 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:1513:8f3a:6d4a:b63]) } 17, 54 -- by smtp.googlemail.com with ESMTPSA id o129sm2715126ito.0.2019.03.19.17.47.47 } 17, 54 -- for {microscopy-at-microscopy.com} } 17, 54 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 17, 54 -- Tue, 19 Mar 2019 17:47:47 -0700 (PDT) } 17, 54 -- Subject: Fwd: Help finding a wax, glue, or M-bond that works at room } 17, 54 -- temperature } 17, 54 -- References: {CAC05CpJFuLGjqRRC0CxtnuXmDzx59pR8hg9Yg+Z=YSj7-X2dQQ-at-mail.gmail.com} } 17, 54 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 17, 54 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 17, 54 -- X-Forwarded-Message-Id: {CAC05CpJFuLGjqRRC0CxtnuXmDzx59pR8hg9Yg+Z=YSj7-X2dQQ-at-mail.gmail.com} } 17, 54 -- Message-ID: {efb6f871-3839-042e-d8b7-732232facb1c-at-gmail.com} } 17, 54 -- Date: Tue, 19 Mar 2019 19:47:47 -0500 } 17, 54 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.14; rv:60.0) } 17, 54 -- Gecko/20100101 Thunderbird/60.5.3 } 17, 54 -- MIME-Version: 1.0 } 17, 54 -- In-Reply-To: {CAC05CpJFuLGjqRRC0CxtnuXmDzx59pR8hg9Yg+Z=YSj7-X2dQQ-at-mail.gmail.com} } 17, 54 -- Content-Type: text/plain; charset=utf-8; format=flowed } 17, 54 -- Content-Language: en-US } 17, 54 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers==============================
==============================Original Headers============================== 7, 46 -- From microwink-at-gmail.com Tue Mar 19 19:55:23 2019 7, 46 -- Received: from mail-io1-f51.google.com (mail-io1-f51.google.com [209.85.166.51]) 7, 46 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x2K0tNgM017980 7, 46 -- for {Microscopy-at-microscopy.com} ; Tue, 19 Mar 2019 19:55:23 -0500 7, 46 -- Received: by mail-io1-f51.google.com with SMTP id x7so477166ioh.4 7, 46 -- for {Microscopy-at-microscopy.com} ; Tue, 19 Mar 2019 18:05:34 -0700 (PDT) 7, 46 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 7, 46 -- d=gmail.com; s=20161025; 7, 46 -- h=mime-version:references:in-reply-to:from:date:message-id:subject:to 7, 46 -- :cc:content-transfer-encoding; 7, 46 -- bh=CadLn0WUPMclCJ9/VHTALUXVkuvdH9VAIuVIRIZt/aI=; 7, 46 -- b=uDTNNEHoCkkHEVPIzdudn69weblaHSm0koXdUQIfNyOIVRRpbt30AYp22tuMh0a6+Q 7, 46 -- wwAX2ohUoNLPWZaYAO/Nbv5YTgDnNVqZms88DpWWQfXNaZTeBxH1bgVkL76qmLH9V8Bk 7, 46 -- gbvCVFbIB6uqyBoqwFbGWptPMST4PAD8ed8dF8UIdTyxRWgYF2Ig9Rj2hDm6No7kM2fK 7, 46 -- aM/Nnsblon+1RF9BJ0EWSfetn5RzpIis+fJezMoW1b/+2WCs8IUIiUM3U/cMQnX2jUn0 7, 46 -- TAphCKE7nUykYEzBpIb+mLEeSMh4QOeX1oVbQ1A9rJ+8MkKYiFuQ8PtdTgYFw7B1pelK 7, 46 -- Stog== 7, 46 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 7, 46 -- d=1e100.net; s=20161025; 7, 46 -- h=x-gm-message-state:mime-version:references:in-reply-to:from:date 7, 46 -- :message-id:subject:to:cc:content-transfer-encoding; 7, 46 -- bh=CadLn0WUPMclCJ9/VHTALUXVkuvdH9VAIuVIRIZt/aI=; 7, 46 -- b=IRv9kHv4xCyWMdeP3KI2cuvIJyfVcLCgEfNRx/4koWINa9Fn05NDP50Fgwqg3XfZSf 7, 46 -- v+lSzn+uStBLed1wK7lqx6PGfUGT7IX05Y24vw2LJhVWI8bXPMpWWuuHmxaZC18ijimb 7, 46 -- EK/PYROPt5UDhqcK22Blh+T4KEMdgeJJMB33TpV25B7mMlOhQBUC30JPPfEf2AmVc1Az 7, 46 -- yvHf2CA4RVpRAfKYRAc2LDabq1YjhJ9Qewf1LDhofOyuwpqx3UEK6HU3BYYFplOzFQUm 7, 46 -- VYlDqy4L5hfcUWB5LIoKVrwYU2t++uL3R87kB0XI2TSUy3kdqN7m7ZdBSVFNMqT8sLdz 7, 46 -- uddQ== 7, 46 -- X-Gm-Message-State: APjAAAXMZYT6ev0DmJc4ApTIRBnZ0s4K0mz3kSgwvN6yZceIC3Ufy+DQ 7, 46 -- 3/kNP+tw3kCtgGtKHxLsHMEaZ8dnvWVjpOlSfoUEyQ== 7, 46 -- X-Google-Smtp-Source: APXvYqwNzY/m6H1wJyzTsyHP1tL7YIWglBXqq+U9PWFXLXbMTdeyVWRHFCEG7CCedI1d+Hxx4QXq4Y5kK32RAzTMwpo= 7, 46 -- X-Received: by 2002:a6b:c909:: with SMTP id z9mr3531443iof.244.1553043934189; 7, 46 -- Tue, 19 Mar 2019 18:05:34 -0700 (PDT) 7, 46 -- MIME-Version: 1.0 7, 46 -- References: {201903200038.x2K0cfrx000365-at-microscopy.com} 7, 46 -- In-Reply-To: {201903200038.x2K0cfrx000365-at-microscopy.com} 7, 46 -- From: Christopher Winkler {microwink-at-gmail.com} 7, 46 -- Date: Tue, 19 Mar 2019 21:05:11 -0400 7, 46 -- Message-ID: {CAA8T2PN8mx5WX+cqdvai5O0ov7Ju+JCB7Kw9T+HhpsxTsOTvoA-at-mail.gmail.com} 7, 46 -- Subject: Re: [Microscopy] Fwd: Help finding a wax, glue, or M-bond that works 7, 46 -- at room 7, 46 -- To: Microscopy-at-microscopy.com 7, 46 -- Cc: rdiazri-at-purdue.edu 7, 46 -- Content-Type: text/plain; charset="UTF-8" 7, 46 -- Content-Transfer-Encoding: 8bit 7, 46 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id x2K0tNgM017980 ==============================End of - Headers==============================
I'd recommend trying a considerably longer purge time. We have an older Tousimis 815 with a smaller chamber (1.25 inches), and our standard purge time is 20 min, extended to 30-35 min for larger samples. By "large" I mean maybe 60x40x30 mm dimensions. It does mean you chew through the liquid CO2, but better than losing precious samples.
cheers, Rosemary
Dr Rosemary White CSIRO Black Mountain GPO Box 1700 Canberra, ACT 2601 Australia
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X-from: becks-at-ncc.edu
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Email: becks-at-ncc.edu Name: Stephen Beck
Organization: Nassau Community College
Title-Subject: [Filtered] Critical Point Dryer Problem
Message: Dear Colleagues,
I have just received a Tousimis 931 critical point dryer to replace a vintage 1992 Polaron Jumbo CPD. I am having problems with residual ethanol in the chamber (large 3.5” diameter) after the completion of the critical point process. Samples are therefore wet and subject to surface tension distortion/collapse. Has anyone else had a similar problem? I have been working with the company tech support and have verified my liquid CO2 tank volume and syphon tube, increased the AUTO purge time from 10 to 15 minutes and verified the metering valve factory settings. Any suggestions are appreciated!
Steve
Stephen J. Beck Professor Coordinator, Bio-Imaging Center Electron Microscopy Department of Biology Nassau Community College Garden City, NY 11530
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Keep checking on eBay. I’ve seen great petrographic and ore microscopes made by Leitz and Zeiss for $1,500. And don’t overlook your local university surplus auctions.  The parts for all these scopes will be available for decades via surplus inventories.
Sent from Mail {https://go.microsoft.com/fwlink/?LinkId=550986} for Windows 10
*From: *microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} *Sent: *Tuesday, March 19, 2019 5:49 PM *To: *cannonmp-at-comcast.net {mailto:cannonmp-at-comcast.net} *Subject: *[Microscopy] Fwd: Good source for used petrographic microscopes
Hello Steve, We had similar problem some years ago, when we have to replace our old Balzers 010 CPD unit. In the new unit there was a minor leak in one valve. We find it out, after careful searching of the drying process. Due to the leak the pressure in the chamber was just bellow or oscillating around the critical pressure value. The unit has an analog pressure meter and it was hard to see it on the analog meter. After replacement of the leaky fitting, all is working fine.
Regards
Oldrich
-- OldĹ™ich Benada Institute of Microbiology, Czech Acad. Sci. Laboratory of Molecular Structure Characterization Electron Microscopy Group VĂdeĹská 1083 142 20 Prague 4 Czech Republic
On Tue, 19 Mar 2019 19:40:12 -0500, microscopy.listserver-at-gmail.com wrote : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } } } -------- Forwarded Message -------- } } } X-from: becks-at-ncc.edu } } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://wwww.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy to both becks-at-ncc.edu, Microscopy-at-Microscopy.com } so that all Microscopy Listserver Subscribers can benefit from our } collective wisdom } --------------------------------------------------------------------------- } } Email: becks-at-ncc.edu Name: Stephen Beck } } Organization: Nassau Community College } } Title-Subject: [Filtered] Critical Point Dryer Problem } } Message: Dear Colleagues, } } I have just received a Tousimis 931 critical point dryer to replace a } vintage 1992 Polaron Jumbo CPD. I am having problems with residual } ethanol in the chamber (large 3.5_ diameter) after the completion of } the critical point process. Samples are therefore wet and subject to } surface tension distortion/collapse. Has anyone else had a similar } problem? I have been working with the company tech support and have } verified my liquid CO2 tank volume and syphon tube, increased the } AUTO purge time from 10 to 15 minutes and verified the metering valve } factory settings. Any suggestions are appreciated! } } Steve } } Stephen J. Beck } Professor } Coordinator, Bio-Imaging Center } Electron Microscopy Department of Biology } Nassau Community College } Garden City, NY 11530 } } Login Host: 173.77.159.14 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original } Headers============================== 18, 53 -- From } microscopy.listserver-at-gmail.com Tue Mar 19 19:39:37 2019 18, 53 -- } Received: from mail-it1-f178.google.com (mail-it1-f178.google.com } [209.85.166.178]) 18, 53 -- by microscopy.com } (8.12.11.20060308/8.12.8) with ESMTP id x2K0dbdN002016 18, 53 -- } for {microscopy-at-microscopy.com} ; Tue, 19 Mar 2019 19:39:37 } -0500 18, 53 -- Received: by mail-it1-f178.google.com with SMTP id } w18so29033638itj.4 18, 53 -- for {microscopy-at-microscopy.com} ; } Tue, 19 Mar 2019 17:49:48 -0700 (PDT) 18, 53 -- DKIM-Signature: v=1; } a=rsa-sha256; c=relaxed/relaxed; 18, 53 -- d=gmail.com; } s=20161025; 18, 53 -- } h=subject:references:to:from:message-id:date:user-agent:mime-version } 18, 53 } -- :in-reply-to:content-language:content-transfer-encoding; } 18, 53 -- bh=PXPB/KW71q+G+tccgxGiZ54fVaKxKr8hLofDlER4ZxE=; } 18, 53 -- } b=bIeItSMYbrHZSpxmRM+1b0lvdV2o8NeqQx1h63k7iDiA9GRJnsBH0/E5ZzUJVTRnKb } 18, 53 -- } RV1/3ujJ311MvqH2rSUZwi2p74/LqThihVIMuSHZuHRFSzupkZ37gx9TFGyWcmzc90c2 } 18, 53 -- } cFllGgWojjrZgctm+3cwplLddJopW1/TtjC8O8x0lAPQrSUqahS9ggfj7QuHkWfsLjYV } 18, 53 -- } VgH37xR+P3Ev5e2yUX8F+WOYCeoF8Guy3zhg2IwHp9/URWhyizCK0HANOt6J8uXt1VnX } 18, 53 -- } UTR8MDuKN89J03fvLUcWRjzzQ9VwgWdxc8P7rus3VYBiOJSH4lbwFsUeGsPyIyWfyjo3 } 18, 53 -- CFgQ== 18, 53 -- X-Google-DKIM-Signature: v=1; } a=rsa-sha256; c=relaxed/relaxed; 18, 53 -- d=1e100.net; } s=20161025; 18, 53 -- } h=x-gm-message-state:subject:references:to:from:message-id:date 18, } 53 -- :user-agent:mime-version:in-reply-to:content-language } 18, 53 -- :content-transfer-encoding; 18, 53 -- } bh=PXPB/KW71q+G+tccgxGiZ54fVaKxKr8hLofDlER4ZxE=; 18, 53 -- } b=hMLzM673odJhFtMGMTUuPgrSJtWnUTvt6LbYxd+jEvp0+UUMrpVzLWbnVM/uIo5kyO } 18, 53 -- } aS0qYx9jbMmG9BsDqQN834Kl+8NEpM7uDCee5wbHVXWysdqsNZTE2Gx3YWYsIByyeziL } 18, 53 -- } ylylotLY3P/0ywfFNVbk+TDnySRP14FBK4HcCalb/HTyGBvc7EJN8PzcljBDt3SyIQ0g } 18, 53 -- } nDZ4kCCDXDhDivQZGWEIdapwVjN2bZtlCYoOkZEguOoacCmEdZZ90h3efG+Zst5Evj11 } 18, 53 -- } qCfhti2o3H/wFddgOn+z5kPV/pBPhdMQG/Rc5udm4WZKieiZW0kHEVjd3AG3gDeCN+So } 18, 53 -- auDA== 18, 53 -- X-Gm-Message-State: } APjAAAWzdPba1mhhEYK+Iv3qoPP7bmGnkqR2rhhezcPvxCLTvpgIdRos 18, 53 -- } q1gtNM/4blzNkFERGRQ8IjSPOlHA 18, 53 -- X-Google-Smtp-Source: } APXvYqyExF42ud/pcJ81NG2LV2sLQyY8s8ubbl5JSAH2sGhcihSUe+fa+PflX1xML1ovxVOFmCDflA== } 18, 53 -- X-Received: by 2002:a24:c842:: with SMTP id } w63mr723210itf.17.1553042988208; 18, 53 -- Tue, 19 Mar 2019 } 17:49:48 -0700 (PDT) 18, 53 -- Received: from } 96-65-115-77-static.hfc.comcastbusiness.net } ([2603:300a:f04:7100:1513:8f3a:6d4a:b63]) 18, 53 -- by } smtp.googlemail.com with ESMTPSA id } u197sm377877itb.9.2019.03.19.17.49.47 18, 53 -- for } {microscopy-at-microscopy.com} 18, 53 -- (version=TLS1_2 } cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 18, 53 -- } Tue, 19 Mar 2019 17:49:47 -0700 (PDT) 18, 53 -- Subject: viaWWW: } Critical Point Dryer Problem 18, 53 -- References: } {201903192110.x2JLACGF020134-at-microscopy.com} 18, 53 -- To: } MicroscopyListServer-Forward {microscopy-at-microscopy.com} 18, 53 -- } From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} 18, 53 } -- X-Forwarded-Message-Id: } {201903192110.x2JLACGF020134-at-microscopy.com} 18, 53 -- Message-ID: } {085c0f41-55d1-31a0-60b6-100dd1cc24b5-at-gmail.com} 18, 53 -- Date: Tue, } 19 Mar 2019 19:49:47 -0500 18, 53 -- User-Agent: Mozilla/5.0 } (Macintosh; Intel Mac OS X 10.14; rv:60.0) 18, 53 -- Gecko/20100101 } Thunderbird/60.5.3 18, 53 -- MIME-Version: 1.0 18, 53 -- In-Reply-To: } {201903192110.x2JLACGF020134-at-microscopy.com} 18, 53 -- Content-Type: } text/plain; charset=windows-1252; format=flowed 18, 53 -- } Content-Language: en-US 18, 53 -- Content-Transfer-Encoding: 8bit } ==============================End of - } Headers==============================
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==============================Original Headers============================== 10, 27 -- From benada-at-biomed.cas.cz Wed Mar 20 03:16:57 2019 10, 27 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) 10, 27 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x2K8Gusj022482 10, 27 -- for {microscopy-at-microscopy.com} ; Wed, 20 Mar 2019 03:16:57 -0500 10, 27 -- Received: from u117ob02 (nb170ph.mbu.cas.cz [147.231.44.133]) 10, 27 -- (using TLSv1.2 with cipher ECDHE-RSA-AES256-GCM-SHA384 (256/256 bits)) 10, 27 -- (No client certificate requested) 10, 27 -- by mail2.biomed.cas.cz (Postfix) with ESMTPSA id 500EFD00F4F 10, 27 -- for {microscopy-at-microscopy.com} ; Wed, 20 Mar 2019 09:27:07 +0100 (CET) 10, 27 -- Date: Wed, 20 Mar 2019 09:27:06 +0100 10, 27 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 10, 27 -- To: microscopy-at-microscopy.com 10, 27 -- Subject: Re: [Microscopy] viaWWW: Critical Point Dryer Problem 10, 27 -- Message-ID: {20190320092706.311ab982-at-u117ob02} 10, 27 -- In-Reply-To: {201903200040.x2K0eC80003174-at-microscopy.com} 10, 27 -- References: {201903200040.x2K0eC80003174-at-microscopy.com} 10, 27 -- Organization: =?UTF-8?B?TWlrcm9iaW9sb2dpY2vDvSDDunN0YXY=?= AV 10, 27 -- =?UTF-8?B?xIxS?= 10, 27 -- X-Mailer: Claws Mail 3.17.1 (GTK+ 2.24.31; i686-pc-linux-gnu) 10, 27 -- MIME-Version: 1.0 10, 27 -- Content-Type: text/plain; charset=UTF-8 10, 27 -- X-IoP-CAS-MailScanner-Information: Please contact the ISP for more information 10, 27 -- X-IoP-CAS-MailScanner-ID: 500EFD00F4F.AA4F7 10, 27 -- X-IoP-CAS-MailScanner: Processed 10, 27 -- X-Spam-Status: No 10, 27 -- Content-Transfer-Encoding: 8bit 10, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id x2K8Gusj022482 ==============================End of - Headers==============================
Ah yes, this rings a distant bell. Not long after we installed our Tousimis CPD (} 10 years ago), we noticed a leak - low final pressure in the chamber and a whistling sound in the CPD. We contacted the Tousimis staff who were very helpful - diagnosing the problem straight away. They sent a replacement part which solved the problem.
cheers, Rosemary
Dr Rosemary White CSIRO Black Mountain GPO Box 1700 Canberra, ACT 2601
T 61 2 6246 5475 M 61 468 966 713 ________________________________________ X-from: benada-at-biomed.cas.cz [benada-at-biomed.cas.cz] Sent: Wednesday, 20 March 2019 7:17 p.m. To: White, Rosemary (A&F, Black Mountain)
Hello Steve, We had similar problem some years ago, when we have to replace our old Balzers 010 CPD unit. In the new unit there was a minor leak in one valve. We find it out, after careful searching of the drying process. Due to the leak the pressure in the chamber was just bellow or oscillating around the critical pressure value. The unit has an analog pressure meter and it was hard to see it on the analog meter. After replacement of the leaky fitting, all is working fine.
Regards
Oldrich
-- Oldřich Benada Institute of Microbiology, Czech Acad. Sci. Laboratory of Molecular Structure Characterization Electron Microscopy Group Vídeňská 1083 142 20 Prague 4 Czech Republic
On Tue, 19 Mar 2019 19:40:12 -0500, microscopy.listserver-at-gmail.com wrote : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } } } -------- Forwarded Message -------- } } } X-from: becks-at-ncc.edu } } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://wwww.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy to both becks-at-ncc.edu, Microscopy-at-Microscopy.com } so that all Microscopy Listserver can benefit from our } collective wisdom } --------------------------------------------------------------------------- } } Email: becks-at-ncc.edu Name: Stephen Beck } } Organization: Nassau Community College } } Title-Subject: [Filtered] Critical Point Dryer Problem } } Message: Dear Colleagues, } } I have just received a Tousimis 931 critical point dryer to replace a } vintage 1992 Polaron Jumbo CPD. I am having problems with residual } ethanol in the chamber (large 3.5_ diameter) after the completion of } the critical point process. Samples are therefore wet and subject to } surface tension distortion/collapse. Has anyone else had a similar } problem? I have been working with the company tech support and have } verified my liquid CO2 tank volume and syphon tube, increased the } AUTO purge time from 10 to 15 minutes and verified the metering valve } factory settings. Any suggestions are appreciated! } } Steve } } Stephen J. Beck } Professor } Coordinator, Bio-Imaging Center } Electron Microscopy Department of Biology } Nassau Community College } Garden City, NY 11530 } } Login Host: 173.77.159.14 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original } Headers============================== 18, 53 -- From } microscopy.listserver-at-gmail.com Tue Mar 19 19:39:37 2019 18, 53 -- } Received: from mail-it1-f178.google.com (mail-it1-f178.google.com } [209.85.166.178]) 18, 53 -- by microscopy.com } (8.12.11.20060308/8.12.8) with ESMTP id x2K0dbdN002016 18, 53 -- } for {microscopy-at-microscopy.com} ; Tue, 19 Mar 2019 19:39:37 } -0500 18, 53 -- Received: by mail-it1-f178.google.com with SMTP id } w18so29033638itj.4 18, 53 -- for {microscopy-at-microscopy.com} ; } Tue, 19 Mar 2019 17:49:48 -0700 (PDT) 18, 53 -- DKIM-Signature: v=1; } a=rsa-sha256; c=relaxed/relaxed; 18, 53 -- d=gmail.com; } s=20161025; 18, 53 -- } h=subject:references:to:from:message-id:date:user-agent:mime-version } 18, 53 } -- :in-reply-to:content-language:content-transfer-encoding; } 18, 53 -- bh=PXPB/KW71q+G+tccgxGiZ54fVaKxKr8hLofDlER4ZxE=; } 18, 53 -- } b=bIeItSMYbrHZSpxmRM+1b0lvdV2o8NeqQx1h63k7iDiA9GRJnsBH0/E5ZzUJVTRnKb } 18, 53 -- } RV1/3ujJ311MvqH2rSUZwi2p74/LqThihVIMuSHZuHRFSzupkZ37gx9TFGyWcmzc90c2 } 18, 53 -- } cFllGgWojjrZgctm+3cwplLddJopW1/TtjC8O8x0lAPQrSUqahS9ggfj7QuHkWfsLjYV } 18, 53 -- } VgH37xR+P3Ev5e2yUX8F+WOYCeoF8Guy3zhg2IwHp9/URWhyizCK0HANOt6J8uXt1VnX } 18, 53 -- } UTR8MDuKN89J03fvLUcWRjzzQ9VwgWdxc8P7rus3VYBiOJSH4lbwFsUeGsPyIyWfyjo3 } 18, 53 -- CFgQ== 18, 53 -- X-Google-DKIM-Signature: v=1; } a=rsa-sha256; c=relaxed/relaxed; 18, 53 -- d=1e100.net; } s=20161025; 18, 53 -- } h=x-gm-message-state:subject:references:to:from:message-id:date 18, } 53 -- :user-agent:mime-version:in-reply-to:content-language } 18, 53 -- :content-transfer-encoding; 18, 53 -- } bh=PXPB/KW71q+G+tccgxGiZ54fVaKxKr8hLofDlER4ZxE=; 18, 53 -- } b=hMLzM673odJhFtMGMTUuPgrSJtWnUTvt6LbYxd+jEvp0+UUMrpVzLWbnVM/uIo5kyO } 18, 53 -- } aS0qYx9jbMmG9BsDqQN834Kl+8NEpM7uDCee5wbHVXWysdqsNZTE2Gx3YWYsIByyeziL } 18, 53 -- } ylylotLY3P/0ywfFNVbk+TDnySRP14FBK4HcCalb/HTyGBvc7EJN8PzcljBDt3SyIQ0g } 18, 53 -- } nDZ4kCCDXDhDivQZGWEIdapwVjN2bZtlCYoOkZEguOoacCmEdZZ90h3efG+Zst5Evj11 } 18, 53 -- } qCfhti2o3H/wFddgOn+z5kPV/pBPhdMQG/Rc5udm4WZKieiZW0kHEVjd3AG3gDeCN+So } 18, 53 -- auDA== 18, 53 -- X-Gm-Message-State: } APjAAAWzdPba1mhhEYK+Iv3qoPP7bmGnkqR2rhhezcPvxCLTvpgIdRos 18, 53 -- } q1gtNM/4blzNkFERGRQ8IjSPOlHA 18, 53 -- X-Google-Smtp-Source: } APXvYqyExF42ud/pcJ81NG2LV2sLQyY8s8ubbl5JSAH2sGhcihSUe+fa+PflX1xML1ovxVOFmCDflA== } 18, 53 -- X-Received: by 2002:a24:c842:: with SMTP id } w63mr723210itf.17.1553042988208; 18, 53 -- Tue, 19 Mar 2019 } 17:49:48 -0700 (PDT) 18, 53 -- Received: from } 96-65-115-77-static.hfc.comcastbusiness.net } ([2603:300a:f04:7100:1513:8f3a:6d4a:b63]) 18, 53 -- by } smtp.googlemail.com with ESMTPSA id } u197sm377877itb.9.2019.03.19.17.49.47 18, 53 -- for } {microscopy-at-microscopy.com} 18, 53 -- (version=TLS1_2 } cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 18, 53 -- } Tue, 19 Mar 2019 17:49:47 -0700 (PDT) 18, 53 -- Subject: viaWWW: } Critical Point Dryer Problem 18, 53 -- References: } {201903192110.x2JLACGF020134-at-microscopy.com} 18, 53 -- To: } MicroscopyListServer-Forward {microscopy-at-microscopy.com} 18, 53 -- } From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} 18, 53 } -- X-Forwarded-Message-Id: } {201903192110.x2JLACGF020134-at-microscopy.com} 18, 53 -- Message-ID: } {085c0f41-55d1-31a0-60b6-100dd1cc24b5-at-gmail.com} 18, 53 -- Date: Tue, } 19 Mar 2019 19:49:47 -0500 18, 53 -- User-Agent: Mozilla/5.0 } (Macintosh; Intel Mac OS X 10.14; rv:60.0) 18, 53 -- Gecko/20100101 } Thunderbird/60.5.3 18, 53 -- MIME-Version: 1.0 18, 53 -- In-Reply-To: } {201903192110.x2JLACGF020134-at-microscopy.com} 18, 53 -- Content-Type: } text/plain; charset=windows-1252; format=flowed 18, 53 -- } Content-Language: en-US 18, 53 -- Content-Transfer-Encoding: 8bit } ==============================End of - } Headers==============================
Upozorneni: Neni-li v teto zprave vyslovne uvedeno jinak, ma tato E-mailova zprava nebo jeji prilohy pouze informativni charakter. Tato zprava ani jeji prilohy v zadnem ohledu ustavy AV CR, v.v.i. k nicemu nezavazuji. Text teto zpravy nebo jejich priloh neni navrhem na uzavreni smlouvy, ani prijetim pripadneho navrhu na uzavreni smlouvy, ani jinym pravnim jednanim smerujicim k uzavreni jakekoliv smlouvy a nezaklada predsmluvni odpovednost ustavu AV CR, v.v.i.
Disclaimer: If not expressly stated otherwise, this e-mail message (including any attached files) is intended purely for informational purposes and does not represent a binding agreement on the part of Institutes of CAS. The text of this message and its attachments cannot be considered as a proposal to conclude a contract, neither the acceptance of a proposal to conclude a contract, nor any other legal act leading to concluding any contract; nor does it create any pre-contractual liability on the part of Institutes of CAS.
==============================Original Headers============================== 10, 27 -- From benada-at-biomed.cas.cz Wed Mar 20 03:16:57 2019 10, 27 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) 10, 27 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x2K8Gusj022482 10, 27 -- for {microscopy-at-microscopy.com} ; Wed, 20 Mar 2019 03:16:57 -0500 10, 27 -- Received: from u117ob02 (nb170ph.mbu.cas.cz [147.231.44.133]) 10, 27 -- (using TLSv1.2 with cipher ECDHE-RSA-AES256-GCM-SHA384 (256/256 bits)) 10, 27 -- (No client certificate requested) 10, 27 -- by mail2.biomed.cas.cz (Postfix) with ESMTPSA id 500EFD00F4F 10, 27 -- for {microscopy-at-microscopy.com} ; Wed, 20 Mar 2019 09:27:07 +0100 (CET) 10, 27 -- Date: Wed, 20 Mar 2019 09:27:06 +0100 10, 27 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 10, 27 -- To: microscopy-at-microscopy.com 10, 27 -- Subject: Re: [Microscopy] viaWWW: Critical Point Dryer Problem 10, 27 -- Message-ID: {20190320092706.311ab982-at-u117ob02} 10, 27 -- In-Reply-To: {201903200040.x2K0eC80003174-at-microscopy.com} 10, 27 -- References: {201903200040.x2K0eC80003174-at-microscopy.com} 10, 27 -- Organization: =?UTF-8?B?TWlrcm9iaW9sb2dpY2vDvSDDunN0YXY=?= AV 10, 27 -- =?UTF-8?B?xIxS?= 10, 27 -- X-Mailer: Claws Mail 3.17.1 (GTK+ 2.24.31; i686-pc-linux-gnu) 10, 27 -- MIME-Version: 1.0 10, 27 -- Content-Type: text/plain; charset=UTF-8 10, 27 -- X-IoP-CAS-MailScanner-Information: Please contact the ISP for more information 10, 27 -- X-IoP-CAS-MailScanner-ID: 500EFD00F4F.AA4F7 10, 27 -- X-IoP-CAS-MailScanner: Processed 10, 27 -- X-Spam-Status: No 10, 27 -- Content-Transfer-Encoding: 8bit 10, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id x2K8Gusj022482 ==============================End of - Headers==============================
We have recently expanded the PyCroscopy package for imaging data analysis to include workflows for deep/machine learning analysis of atomically-resolved data. The new repository is called AI Crystallographer (https://github.com/pycroscopy/AICrystallographer) and it includes interactive Google Colab & Jupyter notebooks with the detailed description of how to run the analysis, as well as pre-trained models for certain type of atomic systems. AI Crystallographer is an active project and we expect to be adding more workflows and pre-trained models in the near future.
The core PyCroscopy package, developed and maintained by Suhas Somnath, Rama Vasudevan and others, is an open source package for storing, visualizing, processing, and analyzing scientific imaging / microscopy data. PyCroscopy takes a data-centric approach, wherein the raw data acquired from the instrument, processed, analyzed, and intermediate results are formatted according to Universal Spectroscopy and Imaging Data (USID) and stored in a single HDF5 file. USID's generalized structure, has facilitated the development and deployment of a variety of instrument-agnostic algorithms available in Pycroscopy.
The AI Crystallographer extension of PyCroscopy aims at bringing the recent developments in the fields of machine learning and computer vision to the microscopy and microanalysis community, with a specific emphasis on the analysis of atomically-resolved data. Currently it includes the following sub-packages:
- DefectNet: Complete workflow for locating atomic defects in electron microscopy movies with a convolutional neural network using only a single movie frame to generate a training set. It is based on paper in npj Computational Materials 5, 12 (2019), but now with the updated augmentation procedure (includes adding noise, zoom-in and flip/rotations) and using PyTorch deep learning framework instead of the Keras one for model training/predictions.
- AtomNet: Application of a fully convolutional neural network for locating atoms in noisy experimental scanning transmission electron microscopy data from graphene. Based on paper in ACS Nano 11, 12742 (2017), but now with a better model (gives "cleaner" predictions) and using PyTorch instead of Keras. The current model is limited to graphene lattice, but we expect to upload more models for different systems in the near future.
- FerroNet: Application of different machine learning and multivariate analysis tools (neural networks, dimensionality reduction, clustering/unmixing) for analysis of ferroic distortions in high-resolution scanning transmission electron microscopy data on perovskites. Based on (soon to be submitted) work.
- SymmetryNet: Application of a deep convolutional network used to determine 2D Bravais lattice symmetry from atomically resolved images. Based on paper in npj Computational Materials 4, 30 (2018).
- Tutorials: Tutorial-like notebooks on using class activation maps for locating defects in the images and using a fully convolutional neural network for cleaning atom-resolved data and locating atoms in it.
We recommend running the notebooks (files with .ipynb extension) in Google Colab, which is a Jupyter notebook environment for machine learning research that requires no setup to use and also provides free GPU/TPU (just click on "open in Colab" icon in a notebook). You should be able to execute the current workflows with your data or perhaps modify/adjust/customize them specifically for your systems (e.g. the current AtomNet is limited to graphene but the tutorial that we provided shows how to train this type of models in general).
If you have any questions or want to report that something is broken (smiley face), the best way to contact us is by filing an issue in AICrystallography GitHub repository.
If interest in using and participating in the development of the AI tools for STEM data analysis, please contact the AI Crystallographer team:
You can find multiple examples of AI Crystallographer applications for real materials systems in our publications (either classical journal, or on arxiv)
Sergei
-- Sergei V. Kalinin
Director, Institute for Functional Imaging of Materials
Fellow MRS, IEEE, APS, IoP, AVS
Oak Ridge National Laboratory
Phone: (865) 241-0236
==============================Original Headers============================== 21, 59 -- From sergei2-at-ornl.gov Wed Mar 20 07:09:06 2019 21, 59 -- Received: from mta01.ornl.gov (mta01.ornl.gov [128.219.177.137]) 21, 59 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x2KC960R012088 21, 59 -- for {microscopy-at-microscopy.com} ; Wed, 20 Mar 2019 07:09:06 -0500 21, 59 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 21, 59 -- d=ornl.gov; i=-at-ornl.gov; q=dns/txt; s=p20151116; 21, 59 -- t=1553084359; x=1584620359; 21, 59 -- h=from:to:cc:subject:date:message-id: 21, 59 -- content-transfer-encoding:mime-version; 21, 59 -- bh=APH2fziZ4a2hmE4yAOxcA8/HH+Gb0PC0cn+nLbJw5Hc=; 21, 59 -- b=ciyFAwc8VYW+UcMx7zgvHcUDs2jtHzIVPtCSf8LfFI2czOo1wQgFW7g3 21, 59 -- 5nX2S+fLVr9wvL8/yvzAQ7eHl+hDllzaZ8IiF+vl34dplqssEcpGKNsFY 21, 59 -- MH8+s2QRe9vqGbRhQUQAFS95kC5LFZBWIBmaPMRdH4n82ucEzw0+XOi4Z 21, 59 -- kiFsf/MMvEWrMcGJ16S0ySOuvqaKAcOJgzsxNyckSpYilfNz7nG9U0a1R 21, 59 -- lrvQyKRV1mMni8fvrXxXuX5beAf9aZAxUnhjFf62aYRGovvHydKckbFZm 21, 59 -- xZj2yvHcAv4YU9l/5djQ4cYVp/nvoD4iEj9XjaQTJ+N1QtA2ZjYC0BhEd 21, 59 -- w==; 21, 59 -- X-SG: RELAYLIST 21, 59 -- X-IronPort-AV: E=Sophos;i="5.60,248,1549947600"; 21, 59 -- d="scan'208";a="61294268" 21, 59 -- Received: from emgwy2.ornl.gov ([160.91.254.10]) 21, 59 -- by iron1.ornl.gov with ESMTP/TLS/DHE-RSA-AES256-GCM-SHA384; 20 Mar 2019 08:19:18 -0400 21, 59 -- Received: from EXCHOS31.ornl.gov (exchos31.ornl.gov [128.219.12.151]) 21, 59 -- (using TLSv1 with cipher ECDHE-RSA-AES256-SHA (256/256 bits)) 21, 59 -- (No client certificate requested) 21, 59 -- by emgwy2.ornl.gov (Postfix) with ESMTPS id 44PTVt6Msdz2SpVV 21, 59 -- for {microscopy-at-microscopy.com} ; Wed, 20 Mar 2019 08:19:18 -0400 (EDT) 21, 59 -- Received: from EXCHCS34.ornl.gov (128.219.12.148) by EXCHOS31.ornl.gov 21, 59 -- (128.219.12.151) with Microsoft SMTP Server (TLS) id 15.0.1395.4; Wed, 20 Mar 21, 59 -- 2019 08:19:18 -0400 21, 59 -- Received: from EXCHOS31.ornl.gov (128.219.12.151) by EXCHCS34.ornl.gov 21, 59 -- (128.219.12.148) with Microsoft SMTP Server (TLS) id 15.0.1395.4; Wed, 20 Mar 21, 59 -- 2019 08:19:17 -0400 21, 59 -- Received: from EXCHOS31.ornl.gov ([fe80::a094:1111:be11:49ad]) by 21, 59 -- EXCHOS31.ornl.gov ([fe80::a094:1111:be11:49ad%17]) with mapi id 21, 59 -- 15.00.1395.000; Wed, 20 Mar 2019 08:19:17 -0400 21, 59 -- From: "Kalinin, Sergei V." {sergei2-at-ornl.gov} 21, 59 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 21, 59 -- CC: "Ziatdinov, Maxim A." {ziatdinovma-at-ornl.gov} , 21, 59 -- "Vasudevan, Rama K." 21, 59 -- {vasudevanrk-at-ornl.gov} , 21, 59 -- "Somnath, Suhas" {somnaths-at-ornl.gov} 21, 59 -- Subject: AI Crystallographer - fully automated atom finding and 21, 59 -- structure/defects/symmetry exploration 21, 59 -- Thread-Topic: AI Crystallographer - fully automated atom finding and 21, 59 -- structure/defects/symmetry exploration 21, 59 -- Thread-Index: AdTfFh3rgOrlyAv6SC6Xp6qMZMxgLA== 21, 59 -- Date: Wed, 20 Mar 2019 12:19:17 +0000 21, 59 -- Message-ID: {f18f67cfcb8645fea5b442e81c4386da-at-EXCHOS31.ornl.gov} 21, 59 -- Accept-Language: en-US 21, 59 -- Content-Language: en-US 21, 59 -- X-MS-Has-Attach: 21, 59 -- X-MS-TNEF-Correlator: 21, 59 -- x-ms-exchange-transport-fromentityheader: Hosted 21, 59 -- x-originating-ip: [128.219.12.135] 21, 59 -- Content-Type: text/plain; charset="us-ascii" 21, 59 -- MIME-Version: 1.0 21, 59 -- Content-Transfer-Encoding: 8bit 21, 59 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id x2KC960R012088 ==============================End of - Headers==============================
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Email: bassimn-at-mcmaster.ca Name: Nabil Bassim
Organization: McMaster University
Title-Subject: [Filtered] Call for Papers/Registration - FIB-SEM Meeting, May 6-7 Washington DC
Message: 12th Annual FIB SEM Workshop Monday, May 6th - Tuesday, May 7th, 2019 George Washington University Washington DC
The 2019 FIB SEM Meeting will be held in May 2019 in the Science and Engineering Hall at the George Washington University in DC. We will have two full days of presentations, tutorials, and posters by FIB users and vendors, highlighting interesting new FIB applications and the latest technology. And, as always, there will be plenty of opportunities for informal discussion of new techniques and applications as well as getting (re)connected with your fellow FIBers.
Abstract Submission Deadline: March 29th, 2019 Abstracts should be 250 words or less in length. Please email your abstracts to fibsem2019-at-fibsem.net Abstract Notification: April 8th, 2019 Registration Deadline: April 19th, 2019 - Register! Travel Info Tentative Agenda Please check our website (www.fibsem.net) for additional information about the meeting. There is no fee to attend the meeting, and breakfast and lunch will be provided! There will be a Happy Hour following the meeting, so don’t forget to click Yes to Happy Hour when you register. Remember, you don't have to be a local user to attend! We look forward to seeing you at the GW! Organizers: Nabil Bassim, bassimn at mcmaster.ca Ken Livi, klivi at jhu.edu Keana Scott, keana.scott at nist.gov Samantha Stambula, stambusm at mcmaster.ca
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-------- Forwarded Message -------- X-from: Kolmakov, Andrei A. (Fed) {andrei.kolmakov-at-nist.gov}
Rosa, have a look on array of NORLAND optical adhesives. https://www.norlandprod.com/UVdefault.html Some of them can be dissolved in acetone. Good luck Andrei
_______________________________ Andrei Kolmakov Ph.D. Project Leader, Nanoscale Imaging Group Nanoscale Device Characterization Div. Physical Measurements Laboratory, NIST 100 Bureau Drive Gaithersburg, MD 20899 MS 6204
-----Original Message----- X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Tuesday, March 19, 2019 8:47 PM To: Kolmakov, Andrei A. (Fed) {andrei.kolmakov-at-nist.gov}
-------- Forwarded Message --------
X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu}
Steve,
Residual EtOH in the chamber most likely means: 1) You didn't completely purge the bulk EtOH from the chamber before starting your soak/purge cycles. 2) You didn't go through critical point, or started venting at critical point (or just below) instead of starting the venting above critical point; recall that the gauges are not exactly accurate and may read a bit high (or be read a bit high if the observer's parallax isn't accounted for), so it is always best to start venting at 33-35 deg C and !200 or so psi. 3) There is a leak as others have suggested - this should be easily detected by noise and by the behavior of the pressure gauge. 4) The heater is not working properly and either not getting above the critical temperature or not maintaining the temperature above critical temperature during vent; this usually results in re-condensation of the CO2, though, not residual EtOH.
Also, unless you're doing something like flattened or monolayer cultured cells, your purge time is *way* too short. Remember, you must replace the EtOH *within* your samples with lqCO2. This is exactly the same as the water =} EtOH dehydration series. This means you must first purge the bulk EtOH, then let the samples sit in lqCO2 at reduced temperature for X minutes, and repeat this Y times, with a ~2 minute purge between soaks to flush out the old lqCO2 (now with EtOH from the sample in it) and replace with fresh lqCO2. X depends on how dense the sample is -- denser tissue means slower diffusion times, which the lqCO2 takes longer to get in and the EtOH longer to get out. Y depends on how much EtOH is in the sample (tissue, etc.) -- more EtOH, more soaks are needed to fully replace the EtOH with lqCO2 -- and how many samples you're running at once; i.e. total sample volume
X and Y are largely empirical.
Phil ------------- Philip Oshel Imaging Facility Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 989 774-3576 office 989 774-7567 lab
Use UV- or vis-light curable cyanoacrylate for attaching sample to (a piece of) microscopy glass slide or cover slip - cure through glass since the sample is opaque. Attach glass with the sample to polishing fixture or holder with epoxy - this bond doesn't need to be removed at RT, so you could heat it up for cleaning later. Polish sample, then dissolve cyanoacrylate in acetone to release sample from the glass. Heat polishing fixture on hot place in a fume hood to release used glass and scrub off the epoxy (sorry, this step is messy).
Good luck! Valery
Valery Ray (also with REFINE Center, UCONN) =========================================== MEO Engineering Company, Inc. DBA "PBS&T" 290 Broadway, Suite 298, Methuen MA 01844 www.partbeamsystech.com www.freudlabs.com Phone: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com
On 3/19/2019 8:37 PM, microscopy.listserver-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } } } -------- Forwarded Message -------- } } } X-from: Rosa Diaz {rdiazri-at-purdue.edu} } } } } } Hello, } } I'm working with a sample that is really sensitive to temperatures above room temperature. } } I need to polish the sample to make a wedge sample for TEM imaging. I need to glue the sample onto a } polisher holder, however i need to find a M-bond, wax, or glue that can be cured at room temperature } and will be hard enough so the sample stays in the holder while polishing! Also, easy to be removed } with a simple solvent. } } The sample is a InSb (semiconductor) substrate. Really brittle and soft as well. } } } Please let me know if you know of any product and thanks in advance. } } Rosa } } /─────────//─────────//─────────//───────//─/ } /Rosa E. Diaz, PhD/ } /Electron Microscopy Research Scientist / } /Birck Nanotechnology Center/ } /Purdue University/ } /1205 W. State Street, West Lafayette, IN 47907-2057 / } /Office: BRK-1272  Tel ://765-496-1075/ } /─────────//─────────//─────────//───────//─/ } } ==============================Original Headers============================== } 17, 54 -- From microscopy.listserver-at-gmail.com Tue Mar 19 19:37:37 2019 } 17, 54 -- Received: from mail-io1-f51.google.com (mail-io1-f51.google.com [209.85.166.51]) } 17, 54 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x2K0bbio031373 } 17, 54 -- for {microscopy-at-microscopy.com} ; Tue, 19 Mar 2019 19:37:37 -0500 } 17, 54 -- Received: by mail-io1-f51.google.com with SMTP id x7so446941ioh.4 } 17, 54 -- for {microscopy-at-microscopy.com} ; Tue, 19 Mar 2019 17:47:49 -0700 (PDT) } 17, 54 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 17, 54 -- d=gmail.com; s=20161025; } 17, 54 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 17, 54 -- :in-reply-to:content-language:content-transfer-encoding; } 17, 54 -- bh=0tausKRgqohi8nbYstZQ2CU727olIfkNh20AHnkaO5E=; } 17, 54 -- b=CAPwMLPNLrUdiAxVJPYiHeBQv4VW7eteK5NTTVxqQIFZj4hv2lX593fL+V4GtctAbM } 17, 54 -- wudNJ1PNU60Cbu1LIPuegkUXr39t0HgJhFIjzJixuN+xbEHJAyPZWvWN/G7TDo9/Z+Ac } 17, 54 -- u+5R+tUjBf5FSbWeb5xQmlwExI077wlHZd7lXqSXibhJiY0nVFB0mh/l6S83pWk5H7pS } 17, 54 -- A2V7Viix6mXt2SQZFg0F3C9ipAZUMIFrsQKkrVyfyvTt3gtEpfQo8I4hq38TTq/2ZxBO } 17, 54 -- mJXmvrr71RQsUw3qE5ZJ2d5jv7X2OHmr7UV7XP+SA/qzmBpy91noqlvn2EfxaWsJVHg0 } 17, 54 -- imbg== } 17, 54 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 17, 54 -- d=1e100.net; s=20161025; } 17, 54 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 17, 54 -- :user-agent:mime-version:in-reply-to:content-language } 17, 54 -- :content-transfer-encoding; } 17, 54 -- bh=0tausKRgqohi8nbYstZQ2CU727olIfkNh20AHnkaO5E=; } 17, 54 -- b=FV73M5qi+fDAotcK81J/NiqUft65SRhY9osZ0+ob6aJbnv2H27YgwhipJk5rDrGkfB } 17, 54 -- gf/7puUpsE9nDUUzaUVxIVb0VfLEfrogUKP2ExlQzrMrqoBFYTrdlxqvxXqX1rUH2gV5 } 17, 54 -- k0jM6BwiLEU2KXjU41Ou46L4iCZj15yRlNm4z+4fPZK2YSaJMthBTDfeqJXCPZyFZjn3 } 17, 54 -- oXYZfKYz7MJbSt4yJO9T5OJaWngEZxTVr1DpRcvsU7gWz3nYFZNbNFmLSgENUzTkQkOT } 17, 54 -- MWxzwRlMsXodPTX79xiondekkWIVGzVWQPCXEQqp7IQ9Ks8JEkP/dWCVL8o7MareCbo1 } 17, 54 -- BpuQ== } 17, 54 -- X-Gm-Message-State: APjAAAVqOd+HbRo86wnkPbGvpzqP0+2q954n76UwV19fcwkFDDykk1OX } 17, 54 -- KRYhr32wdcsiILCE5c3Kqj/5XNgY } 17, 54 -- X-Google-Smtp-Source: APXvYqx4/gQu3rI1HshczdKuHzIXepkz7bBxBxShoUu7LX+aJJdbBy8404PKStOTKEC/pZAWg9Ta5A== } 17, 54 -- X-Received: by 2002:a6b:4e10:: with SMTP id c16mr3306167iob.18.1553042868516; } 17, 54 -- Tue, 19 Mar 2019 17:47:48 -0700 (PDT) } 17, 54 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:1513:8f3a:6d4a:b63]) } 17, 54 -- by smtp.googlemail.com with ESMTPSA id o129sm2715126ito.0.2019.03.19.17.47.47 } 17, 54 -- for {microscopy-at-microscopy.com} } 17, 54 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 17, 54 -- Tue, 19 Mar 2019 17:47:47 -0700 (PDT) } 17, 54 -- Subject: Fwd: Help finding a wax, glue, or M-bond that works at room } 17, 54 -- temperature } 17, 54 -- References: {CAC05CpJFuLGjqRRC0CxtnuXmDzx59pR8hg9Yg+Z=YSj7-X2dQQ-at-mail.gmail.com} } 17, 54 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 17, 54 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 17, 54 -- X-Forwarded-Message-Id: {CAC05CpJFuLGjqRRC0CxtnuXmDzx59pR8hg9Yg+Z=YSj7-X2dQQ-at-mail.gmail.com} } 17, 54 -- Message-ID: {efb6f871-3839-042e-d8b7-732232facb1c-at-gmail.com} } 17, 54 -- Date: Tue, 19 Mar 2019 19:47:47 -0500 } 17, 54 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.14; rv:60.0) } 17, 54 -- Gecko/20100101 Thunderbird/60.5.3 } 17, 54 -- MIME-Version: 1.0 } 17, 54 -- In-Reply-To: {CAC05CpJFuLGjqRRC0CxtnuXmDzx59pR8hg9Yg+Z=YSj7-X2dQQ-at-mail.gmail.com} } 17, 54 -- Content-Type: text/plain; charset=utf-8; format=flowed } 17, 54 -- Content-Language: en-US } 17, 54 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers============================== }
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We have an 815B & 915B. I routinely use purge times of 25-30 minutes for larger volumes of ethanol and bigger/thicker samples. I’ve gone to 40 minutes with some really annoying ptfe grafts that we had a huge amount of trouble getting to dry properly. We have had issues with residual ethanol if our volume of alcohol is high (over 120 mls in the 815B), so I try to keep my ethanol volume as low as possible-just enough to cover our samples.
Deborah
Sent from my iPhone
} On Mar 19, 2019, at 8:52 PM, "microscopy.listserver-at-gmail.com" {microscopy.listserver-at-gmail.com} wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } } } -------- Forwarded Message -------- } } } X-from: becks-at-ncc.edu } } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://wwww.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy to both } becks-at-ncc.edu, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can } benefit from our collective wisdom } --------------------------------------------------------------------------- } } Email: becks-at-ncc.edu Name: Stephen Beck } } Organization: Nassau Community College } } Title-Subject: [Filtered] Critical Point Dryer Problem } } Message: Dear Colleagues, } } I have just received a Tousimis 931 critical point dryer to replace a vintage 1992 Polaron Jumbo } CPD. I am having problems with residual ethanol in the chamber (large 3.5” diameter) after the } completion of the critical point process. Samples are therefore wet and subject to surface tension } distortion/collapse. } Has anyone else had a similar problem? I have been working with the company tech support and have } verified my liquid CO2 tank volume and syphon tube, increased the AUTO purge time from 10 to 15 } minutes and verified the metering valve factory settings. } Any suggestions are appreciated! } } Steve } } Stephen J. Beck } Professor } Coordinator, Bio-Imaging Center } Electron Microscopy Department of Biology } Nassau Community College } Garden City, NY 11530 } } Login Host: 173.77.159.14 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 18, 53 -- From microscopy.listserver-at-gmail.com Tue Mar 19 19:39:37 2019 } 18, 53 -- Received: from mail-it1-f178.google.com (mail-it1-f178.google.com [209.85.166.178]) } 18, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x2K0dbdN002016 } 18, 53 -- for {microscopy-at-microscopy.com} ; Tue, 19 Mar 2019 19:39:37 -0500 } 18, 53 -- Received: by mail-it1-f178.google.com with SMTP id w18so29033638itj.4 } 18, 53 -- for {microscopy-at-microscopy.com} ; Tue, 19 Mar 2019 17:49:48 -0700 (PDT) } 18, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 18, 53 -- d=gmail.com; s=20161025; } 18, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 18, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 18, 53 -- bh=PXPB/KW71q+G+tccgxGiZ54fVaKxKr8hLofDlER4ZxE=; } 18, 53 -- b=bIeItSMYbrHZSpxmRM+1b0lvdV2o8NeqQx1h63k7iDiA9GRJnsBH0/E5ZzUJVTRnKb } 18, 53 -- RV1/3ujJ311MvqH2rSUZwi2p74/LqThihVIMuSHZuHRFSzupkZ37gx9TFGyWcmzc90c2 } 18, 53 -- cFllGgWojjrZgctm+3cwplLddJopW1/TtjC8O8x0lAPQrSUqahS9ggfj7QuHkWfsLjYV } 18, 53 -- VgH37xR+P3Ev5e2yUX8F+WOYCeoF8Guy3zhg2IwHp9/URWhyizCK0HANOt6J8uXt1VnX } 18, 53 -- UTR8MDuKN89J03fvLUcWRjzzQ9VwgWdxc8P7rus3VYBiOJSH4lbwFsUeGsPyIyWfyjo3 } 18, 53 -- CFgQ== } 18, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 18, 53 -- d=1e100.net; s=20161025; } 18, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 18, 53 -- :user-agent:mime-version:in-reply-to:content-language } 18, 53 -- :content-transfer-encoding; } 18, 53 -- bh=PXPB/KW71q+G+tccgxGiZ54fVaKxKr8hLofDlER4ZxE=; } 18, 53 -- b=hMLzM673odJhFtMGMTUuPgrSJtWnUTvt6LbYxd+jEvp0+UUMrpVzLWbnVM/uIo5kyO } 18, 53 -- aS0qYx9jbMmG9BsDqQN834Kl+8NEpM7uDCee5wbHVXWysdqsNZTE2Gx3YWYsIByyeziL } 18, 53 -- ylylotLY3P/0ywfFNVbk+TDnySRP14FBK4HcCalb/HTyGBvc7EJN8PzcljBDt3SyIQ0g } 18, 53 -- nDZ4kCCDXDhDivQZGWEIdapwVjN2bZtlCYoOkZEguOoacCmEdZZ90h3efG+Zst5Evj11 } 18, 53 -- qCfhti2o3H/wFddgOn+z5kPV/pBPhdMQG/Rc5udm4WZKieiZW0kHEVjd3AG3gDeCN+So } 18, 53 -- auDA== } 18, 53 -- X-Gm-Message-State: APjAAAWzdPba1mhhEYK+Iv3qoPP7bmGnkqR2rhhezcPvxCLTvpgIdRos } 18, 53 -- q1gtNM/4blzNkFERGRQ8IjSPOlHA } 18, 53 -- X-Google-Smtp-Source: APXvYqyExF42ud/pcJ81NG2LV2sLQyY8s8ubbl5JSAH2sGhcihSUe+fa+PflX1xML1ovxVOFmCDflA== } 18, 53 -- X-Received: by 2002:a24:c842:: with SMTP id w63mr723210itf.17.1553042988208; } 18, 53 -- Tue, 19 Mar 2019 17:49:48 -0700 (PDT) } 18, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:1513:8f3a:6d4a:b63]) } 18, 53 -- by smtp.googlemail.com with ESMTPSA id u197sm377877itb.9.2019.03.19.17.49.47 } 18, 53 -- for {microscopy-at-microscopy.com} } 18, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 18, 53 -- Tue, 19 Mar 2019 17:49:47 -0700 (PDT) } 18, 53 -- Subject: viaWWW: Critical Point Dryer Problem } 18, 53 -- References: {201903192110.x2JLACGF020134-at-microscopy.com} } 18, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 18, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 18, 53 -- X-Forwarded-Message-Id: {201903192110.x2JLACGF020134-at-microscopy.com} } 18, 53 -- Message-ID: {085c0f41-55d1-31a0-60b6-100dd1cc24b5-at-gmail.com} } 18, 53 -- Date: Tue, 19 Mar 2019 19:49:47 -0500 } 18, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.14; rv:60.0) } 18, 53 -- Gecko/20100101 Thunderbird/60.5.3 } 18, 53 -- MIME-Version: 1.0 } 18, 53 -- In-Reply-To: {201903192110.x2JLACGF020134-at-microscopy.com} } 18, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 18, 53 -- Content-Language: en-US } 18, 53 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers==============================
Not sure if it would work well... but what about using embedding resin (like EMbed-812 from EMS) as glue?...it will cure at room temp (maybe depends on recipe, but recipe used here can cure at room temp. Old resin left in hood for a few days-week is hardened...happy to share recipe if useful), but takes a few days - up to a week to cure at room temp...and can be removed with solvents like propylene oxide...but definitely test it on a backup sample to be sure propylene oxide wouldn't destroy your material, also.
Alternatively, maybe simple cryo-acrylate gel super glue (I find Loctite super glue gel to be easiest to work with and off-gas way less tošnot at all compared to liquid-runny onesš...but there is a chance it would off-gas the white stuff onto your sample if a lot is outside/seeping out from under your sample)...super glue will charge though, but can cover it with colloidal silver liquid or gold or anythingšyou prefer ...and can be removed with acetone which is wayšsafer/preferred by most people than propylene oxide.
Not sure if that's helpful...maybe you've already tried these things...
Emily K. Benson Leadš3DEMšSpecialist & Project Coordinator Renovo Neural Inc. 10000 Cedar Avenue, Cleveland, Ohio, 44106 Office: 216-445-4152 Email: ebenson-at-renovoneural.com Web: www.renovoneural.com ---------------------------------------------------------------------------------------------------- *From:* microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} *Sent:* Tuesday, March 19, 2019 8:46:51 PM *To:* Emily Benson *Subject:* [Microscopy] Fwd: Help finding a wax, glue, or M-bond that works at room
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X-from:š Rosa Diaz {rdiazri-at-purdue.edu}
Hello,
I'm working with a sample that is really sensitive to temperatures above room temperature.
I need to polish the sample to make a wedge sample for TEM imaging. I need to glue the sample onto a polisher holder, however i need to find a M-bond, wax, or glue that can be cured at room temperature and will be hard enough so the sample stays in the holder while polishing! Also, easy to be removed with a simple solvent.
The sample is a InSb (semiconductor) substrate. Really brittle and soft as well.
Please let me know if you know of any product and thanks in advance.
Rosa
/€€€€€€€€€//€€€€€€€€€//€€€€€€€€€//€€€€€€€//€/ /Rosa E. Diaz, PhD/ /Electron Microscopy Research Scientist / /Birck Nanotechnology Center/ /Purdue University/ /1205 W. State Street, West Lafayette, IN 47907-2057 / /Office: BRK-1272 šTel ://765-496-1075/ /€€€€€€€€€//€€€€€€€€€//€€€€€€€€€//€€€€€€€//€/
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Hello Steve, We had similar problem some years ago, when we have to replace our old Balzers 010 CPD unit. In the new unit there was a minor leak in one valve. We find it out, after careful searching of the drying process. Due to the leak the pressure in the chamber was was just bellow or oscillating around the critical pressure value. The unit has an analog pressure meter and it was hard to see it on the analog meter. After replacement of the leaky fitting, all is working fine.
Regards
Oldrich
-- OldĹ™ich Benada Institute of Microbiology, Czech Acad. Sci. Laboratory of Molecular Structure Characterization Electron Microscopy Group VĂdeĹská 1083 142 20 Prague 4 Czech Republic
On Tue, 19 Mar 2019 19:40:12 -0500, microscopy.listserver-at-gmail.com wrote : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } } } -------- Forwarded Message -------- } } } X-from: becks-at-ncc.edu } } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://wwww.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy to both becks-at-ncc.edu, Microscopy-at-Microscopy.com } so that all Microscopy Listserver Subscribers can benefit from our } collective wisdom } --------------------------------------------------------------------------- } } Email: becks-at-ncc.edu Name: Stephen Beck } } Organization: Nassau Community College } } Title-Subject: [Filtered] Critical Point Dryer Problem } } Message: Dear Colleagues, } } I have just received a Tousimis 931 critical point dryer to replace a } vintage 1992 Polaron Jumbo CPD. I am having problems with residual } ethanol in the chamber (large 3.5_ diameter) after the completion of } the critical point process. Samples are therefore wet and subject to } surface tension distortion/collapse. Has anyone else had a similar } problem? I have been working with the company tech support and have } verified my liquid CO2 tank volume and syphon tube, increased the } AUTO purge time from 10 to 15 minutes and verified the metering valve } factory settings. Any suggestions are appreciated! } } Steve } } Stephen J. Beck } Professor } Coordinator, Bio-Imaging Center } Electron Microscopy Department of Biology } Nassau Community College } Garden City, NY 11530 } } Login Host: 173.77.159.14 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original } Headers============================== 18, 53 -- From } microscopy.listserver-at-gmail.com Tue Mar 19 19:39:37 2019 18, 53 -- } Received: from mail-it1-f178.google.com (mail-it1-f178.google.com } [209.85.166.178]) 18, 53 -- by microscopy.com } (8.12.11.20060308/8.12.8) with ESMTP id x2K0dbdN002016 18, 53 -- } for {microscopy-at-microscopy.com} ; Tue, 19 Mar 2019 19:39:37 } -0500 18, 53 -- Received: by mail-it1-f178.google.com with SMTP id } w18so29033638itj.4 18, 53 -- for {microscopy-at-microscopy.com} ; } Tue, 19 Mar 2019 17:49:48 -0700 (PDT) 18, 53 -- DKIM-Signature: v=1; } a=rsa-sha256; c=relaxed/relaxed; 18, 53 -- d=gmail.com; } s=20161025; 18, 53 -- } h=subject:references:to:from:message-id:date:user-agent:mime-version } 18, 53 } -- :in-reply-to:content-language:content-transfer-encoding; } 18, 53 -- bh=PXPB/KW71q+G+tccgxGiZ54fVaKxKr8hLofDlER4ZxE=; } 18, 53 -- } b=bIeItSMYbrHZSpxmRM+1b0lvdV2o8NeqQx1h63k7iDiA9GRJnsBH0/E5ZzUJVTRnKb } 18, 53 -- } RV1/3ujJ311MvqH2rSUZwi2p74/LqThihVIMuSHZuHRFSzupkZ37gx9TFGyWcmzc90c2 } 18, 53 -- } cFllGgWojjrZgctm+3cwplLddJopW1/TtjC8O8x0lAPQrSUqahS9ggfj7QuHkWfsLjYV } 18, 53 -- } VgH37xR+P3Ev5e2yUX8F+WOYCeoF8Guy3zhg2IwHp9/URWhyizCK0HANOt6J8uXt1VnX } 18, 53 -- } UTR8MDuKN89J03fvLUcWRjzzQ9VwgWdxc8P7rus3VYBiOJSH4lbwFsUeGsPyIyWfyjo3 } 18, 53 -- CFgQ== 18, 53 -- X-Google-DKIM-Signature: v=1; } a=rsa-sha256; c=relaxed/relaxed; 18, 53 -- d=1e100.net; } s=20161025; 18, 53 -- } h=x-gm-message-state:subject:references:to:from:message-id:date 18, } 53 -- :user-agent:mime-version:in-reply-to:content-language } 18, 53 -- :content-transfer-encoding; 18, 53 -- } bh=PXPB/KW71q+G+tccgxGiZ54fVaKxKr8hLofDlER4ZxE=; 18, 53 -- } b=hMLzM673odJhFtMGMTUuPgrSJtWnUTvt6LbYxd+jEvp0+UUMrpVzLWbnVM/uIo5kyO } 18, 53 -- } aS0qYx9jbMmG9BsDqQN834Kl+8NEpM7uDCee5wbHVXWysdqsNZTE2Gx3YWYsIByyeziL } 18, 53 -- } ylylotLY3P/0ywfFNVbk+TDnySRP14FBK4HcCalb/HTyGBvc7EJN8PzcljBDt3SyIQ0g } 18, 53 -- } nDZ4kCCDXDhDivQZGWEIdapwVjN2bZtlCYoOkZEguOoacCmEdZZ90h3efG+Zst5Evj11 } 18, 53 -- } qCfhti2o3H/wFddgOn+z5kPV/pBPhdMQG/Rc5udm4WZKieiZW0kHEVjd3AG3gDeCN+So } 18, 53 -- auDA== 18, 53 -- X-Gm-Message-State: } APjAAAWzdPba1mhhEYK+Iv3qoPP7bmGnkqR2rhhezcPvxCLTvpgIdRos 18, 53 -- } q1gtNM/4blzNkFERGRQ8IjSPOlHA 18, 53 -- X-Google-Smtp-Source: } APXvYqyExF42ud/pcJ81NG2LV2sLQyY8s8ubbl5JSAH2sGhcihSUe+fa+PflX1xML1ovxVOFmCDflA== } 18, 53 -- X-Received: by 2002:a24:c842:: with SMTP id } w63mr723210itf.17.1553042988208; 18, 53 -- Tue, 19 Mar 2019 } 17:49:48 -0700 (PDT) 18, 53 -- Received: from } 96-65-115-77-static.hfc.comcastbusiness.net } ([2603:300a:f04:7100:1513:8f3a:6d4a:b63]) 18, 53 -- by } smtp.googlemail.com with ESMTPSA id } u197sm377877itb.9.2019.03.19.17.49.47 18, 53 -- for } {microscopy-at-microscopy.com} 18, 53 -- (version=TLS1_2 } cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 18, 53 -- } Tue, 19 Mar 2019 17:49:47 -0700 (PDT) 18, 53 -- Subject: viaWWW: } Critical Point Dryer Problem 18, 53 -- References: } {201903192110.x2JLACGF020134-at-microscopy.com} 18, 53 -- To: } MicroscopyListServer-Forward {microscopy-at-microscopy.com} 18, 53 -- } From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} 18, 53 } -- X-Forwarded-Message-Id: } {201903192110.x2JLACGF020134-at-microscopy.com} 18, 53 -- Message-ID: } {085c0f41-55d1-31a0-60b6-100dd1cc24b5-at-gmail.com} 18, 53 -- Date: Tue, } 19 Mar 2019 19:49:47 -0500 18, 53 -- User-Agent: Mozilla/5.0 } (Macintosh; Intel Mac OS X 10.14; rv:60.0) 18, 53 -- Gecko/20100101 } Thunderbird/60.5.3 18, 53 -- MIME-Version: 1.0 18, 53 -- In-Reply-To: } {201903192110.x2JLACGF020134-at-microscopy.com} 18, 53 -- Content-Type: } text/plain; charset=windows-1252; format=flowed 18, 53 -- } Content-Language: en-US 18, 53 -- Content-Transfer-Encoding: 8bit } ==============================End of - } Headers==============================
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Email: eg5-at-ornl.gov Name: Andres Marquez
Organization: Oak Ridge National Laboratory
Title-Subject: [Filtered] EMI and Vibration Testing
Message: I am in need of a company that can do EMI and Vibration testing at ORNL in order to help us out with the installation of characterization equipment like SEM's. Login Host: 160.91.114.224 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
Directly reporting to the Manager of the Imaging and Analysis group and the faculty director for the Center for Nanoscale Systems.  Indirectly reporting to other imaging staff members, the student user community and their respective faculty advisors.
This person will be responsible for biological electron microscopy, support
, and training and to provide direct research support for the faculty and the researchers at the Center for Nanoscale Systems.  This person will also be directly responsible for overseeing the maintenance and operation of several electron microscopes in the facility.  Must be fully proficient in the operation of Transmission Electron Microscopes (TEM) and Scanning Electron microscopes (SEM).  Maintenance of the biological sample preparation facility and must be fully proficient in biological sample preparation techniques for electron microscopy such as; ultra-microtome, critical point dryer and the CEVS plunge freeze system.
Must also be proficient in biological sample preparation such as staining, negative staining, fixation and embedding samples both for SEM and TEM observation.  Independently perform scientific investigative procedures requiring application of professional judgment. Interpret experimental results and determine whether they are consistent with experimental goals. Review pertinent literature for information that will further research goals. Prepare reports of research, protocols and SOP's.  Must be willing to have BL2 certification and work with blood born pathogens and keep current with required safety training.  Actively contribute to the research results of the facility and have an active role in project development and execution including managing routine aspects of research projects.  Identify and work toward solutions of methodological problems, and train others in complex technical protocols and use of equipment.  This person will also be charge of educational outreach activities regarding biological imaging.
Must be able to lift and handle liquid nitrogen dewars, fill cryo-pumping systems and move liquid nitrogen containers to place of research from filling stations.
Basic Qualifications
*Â Â Â Â Â Â Â Â Â Â Â Â A minimum of a bachelor's degree in an appropriate field of s=
cience or technology.
*Â Â Â Â Â Â Â Â Â Â Â Â A minimum of four years of related research experience in an =
biological electron microscopy laboratory environment
Additional Qualifications
*Â Â Â Â Â Â Â Â Â Â Â Â Advanced degree in a related field strongly preferred
*Â Â Â Â Â Â Â Â Â Â Â Â The candidate should be self-motivated with a strong desire t=
o further develop biological electron microscopy techniques and develop fur=
ther professional practical skills.
*Â Â Â Â Â Â Â Â Â Â Â Â Excellent documenting and English written skills required.
*Â Â Â Â Â Â Â Â Â Â Â Â The demonstrated ability to work well in teams is essential.
-----Original Message----- X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Wednesday, March 27, 2019 7:42 AM To: Michael Brazil {mbrazil-at-vergason.com}
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Email: eg5-at-ornl.gov Name: Andres Marquez
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Title-Subject: [Filtered] EMI and Vibration Testing
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Email: james.r.mcbride-at-vanderbilt.edu Name: James R. McBride
Organization: Vanderbilt University
Title-Subject: [Filtered] TEM Plan View Single Crystal Si Samples
Message: I am teaching an electron microscopy class and I'm in need of a few single crystal/oriented silicon samples for demonstrating CBED and Kikuchi maps in the TEM.
1) Anyone have extras lying around??? (wishful thinking)
2) Any recommendations for an accessible sample preparation method?
Thanks!
James
James R. McBride Research Associate Professor Chemistry Department/VINSE/EE Vanderbilt University james.r.mcbride-at-vanderbilt.edu
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The Central Facility for Advanced Microscopy and Microanalysis (CFAMM) at the University of California at Riveside has an open position for electron microscopy laboratory technician. Position responsibilities include operation and routine maintenance of electron microscopes, FIB instrument, and all phases of sample preparation and analysis for various types of materials. This position includes training users on specimen preparation, microscope operation, and related techniques.
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Email: ravi.thakkar369-at-gmail.com Name: Ravi
Title-Subject: [Filtered] Processing exosome for ultramicrotomy.
Message: Hi all, I have user who is interested in the internal structure of exosome. But sample is very minute, even the pallet is not visible. Can anybody guide me how to prepare exosome sample for ultramicrotomy?
Ravi.
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Title-Subject: [Filtered] TEM: practical aspects of Schottky and (cold) field emission sources
Message: Dear Listers, may anyone produce a short comparison of practical advantages/drawbacks of Schottky vs (so-called cold) field emission sources in TEM? I mean, I have an idea about characteristics of each of them, but no parctical experience. For istance: life time, robustness against gun discharges and mains black-outs, operation stability against vacuum fluctuations (especially for (C)FE), sensitivity to stray em fields and vibrations, running costs, etc. Moreover, are Schottky better than (C)FE for EDS?
I know practical advice = advice from a colleague with practical experience perhaps is expected but unfortunately I can't provide such for you. On the other hand it is not unwise (at least until the 'practical experiences' are given and sent to you) to point to some scientific articles for reading about technique one could reproduce or at least adapt (& thinking about parameters given or might be of importance)
https://www.researchgate.net/publication/322269064_Sample_Preparation_and_Im aging_of_Exosomes_by_Transmission_Electron_Microscopy Video Article JUNG, M.K., Mun, J.Y., 2018 Sample Preparation and Imaging of Exosomes by Transmission Electron Microscopy. J. Vis. Exp. (131), e56482, URL: https://www.researchgate.net/deref/https%3A%2F%2Fwww.jove.com%2Fvideo%2F5648 2 (PDF) Sample Preparation and Imaging of Exosomes by Transmission Electron Microscopy. Available from: https://www.researchgate.net/publication/322269064_Sample_Preparation_and_Im aging_of_Exosomes_by_Transmission_Electron_Microscopy [accessed Mar 30 2019].OR SEE also: JUNG, M.K., Mun, J.Y., 2018 Sample Preparation and Imaging of Exosomes by Transmission Electron Microscopy. doi:10.3791/56482 (2018) cf.: https://www.jove.com/video/56482/sample-preparation-imaging-exosomes-transmi ssion-electron
= https://www.ncbi.nlm.nih.gov/pubmed/29364263
CHOI and MUN, 2017 Structural Analysis of Exosomes Using Different Types of Electron Microscopy pISSN 2287-5123·eISSN 2287-4445 https://doi.org/10.9729/AM.2017.47.3.171 https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=5&ved=2ahUKEwi w07fA9anhAhVyhosKHcT3AM0QFjAEegQIBRAC&url=http%3A%2F%2Fwww.appmicro.org%2Fjo urnal%2Fdownload_pdf.php%3Fdoi%3D10.9729%2FAM.2017.47.3.171&usg=AOvVaw0K3R2f vfPEmnyba2zMATVf
WU et al.,2015 Exosomes: improved methods to characterize theirmorphology, RNA content, and surface proteinbiomarkers -at- http://oww-files-public.s3.amazonaws.com/3/34/ExosomeAnalyst.pdf
X-from: Norwegian University of Science and Technology Side 1 'SamplepreparationforTransmissionelectronmicroscopy(TEM)' https://www.ntnu.edu/documents/139994/141053151/TEM+sample+preparation.pdf/e b6c557f-8243-4923-9135-cc8f8fa5c37f
Also, perhaps have a look into Exosome-TEM-easy Kit - 101Bio.com www.101bio.com/P130.php Exosome-TEM-easy Kit (prepare exosome for transmission electron microscopy imaging) Application: This kit is for preparing exosome samples for transmission electron microscopy imaging assay. This product is for research use only. Description: This is an easy-to-use tool helps you to prepare exosome samples for transmission electron microscope imaging, and to get good quality EM images of exosome structure. This kit contains all reagents and material for the experiment. The exosome purity and density is important for TEM assay. We recommend to use our PureExo kits to isolate exosomes before using this kit. Following: Manual of operation (http://www.101bio.com/files/manual_P130.pdf), some lit. references, components used/needed, additional required material and See: http://www.101bio.com/exosome_product.php (multiple ads for exosome kits products) (Also available from FISHER, VWR,....)
NB and Disclaimer: No affiliation or financial interest in any mentioned company No personal warranty for sufficiency of the mentioned article(s), preparation method/technique presented, etc.etc....
Best wishes and good luck, Wolfgang
MUSS Wolfgang (PhD) [OR i. R. / retired] Ignaz-Rieder-Kai 19/6 A-5020 SALZBURG Österreich-AUSTRIA E-Mail: womuss-at-gmail.com
FRMS, Retired Member of MSA Scientific Profile at ResearchGate: http://www.researchgate.net/profile/Wolfgang_MUSS Former Head of Electron Microscopy Lab at Institute of Pathology SALK-LKH / Salzburger Landeskliniken | General Hospital and PMU (private) PARACELSUS MEDICAL UNIVERSITY of SALZBURG
Former Secretary and (until June2017) Board Member of the
SCUR {The Society for Cutaneous Ultrastructure Research} „The Skin Imaging Society“ { www.scur.org }
============================================================================ ============================ Von: zaluzec-at-microscopy.com [mailto:zaluzec-at-microscopy.com] Gesendet: Samstag, 30. März 2019 01:17 An: wij.muss-at-aon.at Betreff: [Microscopy] Processing exosome for ultramicrotomy
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Hi all,
I have user who is interested in the internal structure of exosome. But sample is very minute, even the pallet [sic! pellet?] is not visible. Can anybody guide me how to prepare exosome sample for ultramicrotomy?
Ravi.
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X-from: Ribardičre Michel {m.ribardiere-at-jeol.fr}
Hello
As far as we have expérience in TEM, cold or shottky gun have about same Life time even if we had very long shottky gun Life time (record of 11 years)
There is no difference about EDS sensitivity. The main advantage of cold gun is the obtainable résolution in EELS Thé disavantage is the stability in term of émission. The emitter tip bas To .be de-contaninated twice per day , but Eds system includes now ŕ monitoring of émission. The level of vacuum in cold FEG Also needs To .be better than shottky type (10-7 Pa for shottky and 10-9Pa for cold) The level of magnetic field is more important if you use cold FEG. But it dépends on thé level. I hope it helps you
Envoyé depuis mon appareil mobile Samsung.
-------- Message d'origine -------- De : zaluzec-at-microscopy.com Date : 30/03/2019 01:28 (GMT+01:00) Ŕ : Ribardičre Michel {m.ribardiere-at-jeol.fr} Objet : [Microscopy] viaWWW:TEM: practical aspects of Schottky and (cold) field emission
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Title-Subject: [Filtered] TEM: practical aspects of Schottky and (cold) field emission sources
Message: Dear Listers, may anyone produce a short comparison of practical advantages/drawbacks of Schottky vs (so-called cold) field emission sources in TEM? I mean, I have an idea about characteristics of each of them, but no parctical experience. For istance: life time, robustness against gun discharges and mains black-outs, operation stability against vacuum fluctuations (especially for (C)FE), sensitivity to stray em fields and vibrations, running costs, etc. Moreover, are Schottky better than (C)FE for EDS?
We have recently had users bring us exosomes for ultramicrotomy that have been FACS sorted, so there are very few. There is no visible pellet.
I have the user spin them down as hard as they can in an eppendorf tube and fix them. We do all the processing and embedding in the tube and polymerize.
I will then section the bottom of the tube without taking any thick sections at all and pick up small round sections immediately. There are usually enough exosomes at the bottom and along the outside if the circle as you begin to section in to get an adequate number of images for my user.
Good luck.
Leslie Leslie Gunther Cummins
Albert Einstein College of Medicine Analytical Imaging Facility 1300 Morris Park Ave, Forch 639 Bronx, NY 10461 718-430-3547
------------------
Email: ravi.thakkar369-at-gmail.com Name: Ravi
Title-Subject: [Filtered] Processing exosome for ultramicrotomy.
Message: Hi all, I have user who is interested in the internal structure of exosome. But sample is very minute, even the pallet is not visible. Can anybody guide me how to prepare exosome sample for ultramicrotomy?
Almost exactly a year ago, my Lego microscope collection came up on a thread on the listserv:
https://www.mta.ca/dmf/lego.html
I had several requests for part lists and building instructions, which I made a vague promise to do over the summer of 2018. Well, like all great plans, that didn't happen. I finally got back to looking at the task last week, and was depressed at the thought of taking all the models apart a piece at a time, photographing each step, coming up with a parts list, etc. Then I discovered "Lego Digital Designer", a free CAD-like program for building virtual Lego:
https://www.lego.com/en-us/ldd
(Never mind the warnings about it not being supported and possible "licensing errors" on startup - still an easy to learn and great program). The best thing - once a model is virtually constructed, it can be saved as building instructions, in HTML! Complete with parts for each step *and* a parts list at the end (including part numbers) so one can hunt down all the parts needed.
So far I've only done the light microscope model:
https://www.mta.ca/dmf/lego_lm.html
If you scroll to the bottom of the image, a link will take you to the building instructions. I built the model exactly as the put together by my son years ago (and shown in the photo) but I'm sure those interested can come up with some cool modifications.
Enjoy! And let me know what you think. I'll be adding instructions for the other models as time allows. Hopefully on a timeline sooner than the original promise ;-)
Cheers,
Jim
--
James M. Ehrman Digital Microscopy Facility Mount Allison University 63B York St. Sackville, NB E4L 1G7 CANADA
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Hi Jim I love your models. They will make a great addition to the Outreach Booth at M&M if I can figure out how to put them together. I will be sure to put your name on the designs.
When I put the challenge of "The Great Lego Microscope Competition" for the Outreach Booth at M&M, it was to make a working model with a smart phone as the camera. I based mine on the "Echo Wooden Microscope" https://www.youtube.com/watch?v=lbCW-tCscAk So many ways to make it!
The challenge is there for M&M 2019 in Portland for anyone who wants to participate and bring their model!
Many thanks for this update Best wishes Elaine
Dr. Elaine C. Humphrey Fellow of Microscopy Society of America Past President Microscopical Society of Canada
Advanced Microscopy Facility Bob Wright Science Centre A015 University of Victoria, Canada
Almost exactly a year ago, my Lego microscope collection came up on a thread on the listserv:
https://www.mta.ca/dmf/lego.html
I had several requests for part lists and building instructions, which I made a vague promise to do over the summer of 2018. Well, like all great plans, that didn't happen. I finally got back to looking at the task last week, and was depressed at the thought of taking all the models apart a piece at a time, photographing each step, coming up with a parts list, etc. Then I discovered "Lego Digital Designer", a free CAD-like program for building virtual Lego:
https://www.lego.com/en-us/ldd
(Never mind the warnings about it not being supported and possible "licensing errors" on startup - still an easy to learn and great program). The best thing - once a model is virtually constructed, it can be saved as building instructions, in HTML! Complete with parts for each step *and* a parts list at the end (including part numbers) so one can hunt down all the parts needed.
So far I've only done the light microscope model:
https://www.mta.ca/dmf/lego_lm.html
If you scroll to the bottom of the image, a link will take you to the building instructions. I built the model exactly as the put together by my son years ago (and shown in the photo) but I'm sure those interested can come up with some cool modifications.
Enjoy! And let me know what you think. I'll be adding instructions for the other models as time allows. Hopefully on a timeline sooner than the original promise ;-)
Cheers,
Jim
--
James M. Ehrman Digital Microscopy Facility Mount Allison University 63B York St. Sackville, NB E4L 1G7 CANADA
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Email: cannonmp-at-comcast.net Name: Bart Cannon
Organization: Cannon Microprobe
Title-Subject: [Filtered] PGT Avalon 8000 (8K)
Message: I am trying to locate a PGT Avalon 8000 x-ray pulse processor / SEM scan control for digital imaging. I will pay a fair price or I can trade from my extensive suite of electron probe standards and reference materials.
I got my first electron probe in 1983 and I've been in "legacy equipment purgatory" ever since.
There has been such a series of mega buyouts and marketing fever that most things are already legacy by the time you buy them new.
PGT gear is in thousands of labs, but Thermo bought them and then Thermo dropped the whole line and there was no transfer of staff.
Someone's ultra thin x-ray detector window will fail today.
I am looking for a light element detecting windowless x-ray detector. Ketek and Amptek claim their detectors will run naked to the vacuum. I tried it and failed, but I hope I live to see windowless detectors, even for proportional counters.
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There is a microprobe-specific listserver that is still alive, maybe it could also be of help:
https://lists.umn.edu/cgi-bin/wa?A0=probeusers
Valery
Valery Ray (also with REFINE Center, UCONN) =========================================== MEO Engineering Company, Inc. DBA "PBS&T" 290 Broadway, Suite 298, Methuen MA 01844 www.partbeamsystech.com www.freudlabs.com Phone: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com
On 4/2/2019 6:48 AM, microscopy.listserver-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } -------- Forwarded Message -------- } } X-from: cannonmp-at-comcast.net } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy to both } cannonmp-at-comcast.net, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can } benefit from our collective wisdom } --------------------------------------------------------------------------- } } Email: cannonmp-at-comcast.net Name: Bart Cannon } } Organization: Cannon Microprobe } } Title-Subject: [Filtered] PGT Avalon 8000 (8K) } } Message: I am trying to locate a PGT Avalon 8000 x-ray pulse processor / SEM scan control for } digital imaging. I will pay a fair price or I can trade from my extensive suite of electron probe } standards and reference materials. } } I got my first electron probe in 1983 and I've been in "legacy equipment purgatory" ever since. } } There has been such a series of mega buyouts and marketing fever that most things are already legacy } by the time you buy them new. } } PGT gear is in thousands of labs, but Thermo bought them and then Thermo dropped the whole line and } there was no transfer of staff. } } Someone's ultra thin x-ray detector window will fail today. } } I am looking for a light element detecting windowless x-ray detector. Ketek and Amptek claim their } detectors will run naked to the vacuum. I tried it and failed, but I hope I live to see windowless } detectors, even for proportional counters. } } Login Host: 73.97.160.47 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 15, 53 -- From microscopy.listserver-at-gmail.com Tue Apr 2 05:48:26 2019 } 15, 53 -- Received: from mail-it1-f182.google.com (mail-it1-f182.google.com [209.85.166.182]) } 15, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x32AmQEb018285 } 15, 53 -- for {microscopy-at-microscopy.com} ; Tue, 2 Apr 2019 05:48:26 -0500 } 15, 53 -- Received: by mail-it1-f182.google.com with SMTP id w15so4454709itc.0 } 15, 53 -- for {microscopy-at-microscopy.com} ; Tue, 02 Apr 2019 03:59:22 -0700 (PDT) } 15, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 15, 53 -- d=gmail.com; s=20161025; } 15, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 15, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 15, 53 -- bh=9SEE7EqpdaZHM2ji9g1AFn2MFvk+lMVx/J9Y+5EUXpk=; } 15, 53 -- b=OVmNF6uVBIgcRbKl6ZMd3EJm28dozd/kot7S4QuSmAvglmtGWbK1LBRUkUhYkJM7Z+ } 15, 53 -- 4uon8E8bTLufz0aF7k94MWiQYmQtHAookVK7dq4DkTG3Nl7ql6A7k5qxOiPTER6E1GCu } 15, 53 -- Xcw9rHT4sbrhkF93J2xT+w/iOEmuDE3mx1F+hjlKcY1Dqb4GeNTpyOywmWKXCFCfZ6cU } 15, 53 -- GRdSZ0Q3+IXzpOJ7Q6WXx8U9fQuEicQj/PQF6i5Cf1KD4CYwuH4zXjavSb6A4qJPh3Xd } 15, 53 -- 7OO8jfTUyC2rInD04TTa8528YpM1G8GaGC7z/mB97pREMhdJ6S12o/AAcLeqkmST4/7j } 15, 53 -- ZkHg== } 15, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 15, 53 -- d=1e100.net; s=20161025; } 15, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 15, 53 -- :user-agent:mime-version:in-reply-to:content-language } 15, 53 -- :content-transfer-encoding; } 15, 53 -- bh=9SEE7EqpdaZHM2ji9g1AFn2MFvk+lMVx/J9Y+5EUXpk=; } 15, 53 -- b=pl71/HMA+iZqb1xg23fBlcQJj/Mm8aYK4iXtVbtH6ZUMMJjkAxqoaO2PXftj19dBNf } 15, 53 -- 96bg9yIlXdEzW6XDkLVCijc0i7zDeksQHdn0MkCbGXwtDykIcH9WaOJFtHOoTLBRqFJH } 15, 53 -- PSfoTs78G9CJ++88E+nEzopQXCM3LViWTkOEdq2zqKdIur3lyB4VTh1WEjLS5iMdPs37 } 15, 53 -- +JDT/c1pd3xnxsCVZ26UXyxLXKPliI2Y5oQUcjyfazbu8Tx1QXdfz7GAtc1z4qa2Ygn7 } 15, 53 -- nUovdsbc95jfbxa/YxjmxrKaN5XnC+zJhQIoGm9mGJYOBr6e7gWoV0yCIme1AkfeWKWx } 15, 53 -- N2cQ== } 15, 53 -- X-Gm-Message-State: APjAAAXhZqaCiEUj0CtqYhujWFg8qTg6ucuX7jmd0Js89OAnezWtRVJT } 15, 53 -- 7h28I4QBqmIGD/PK1MYgstXFTw0m } 15, 53 -- X-Google-Smtp-Source: APXvYqyPG+eicb3v6ZERA6QJIgro+fZmL3vB6smVPT0CLjTNY4GmMvtvB1I4LNnxMMawD+zupENUxg== } 15, 53 -- X-Received: by 2002:a24:d88:: with SMTP id 130mr3234556itx.87.1554202761688; } 15, 53 -- Tue, 02 Apr 2019 03:59:21 -0700 (PDT) } 15, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:4d1f:f578:5df9:7183]) } 15, 53 -- by smtp.googlemail.com with ESMTPSA id s5sm5227562iog.34.2019.04.02.03.59.20 } 15, 53 -- for {microscopy-at-microscopy.com} } 15, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 15, 53 -- Tue, 02 Apr 2019 03:59:20 -0700 (PDT) } 15, 53 -- Subject: viaWWW:PGT Avalon 8000 (8K) } 15, 53 -- References: {201904012351.x31Npw2e024329-at-microscopy.com} } 15, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 15, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 15, 53 -- X-Forwarded-Message-Id: {201904012351.x31Npw2e024329-at-microscopy.com} } 15, 53 -- Message-ID: {a801eb7b-5549-de90-adbc-7084605672d8-at-gmail.com} } 15, 53 -- Date: Tue, 2 Apr 2019 05:59:19 -0500 } 15, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.14; rv:60.0) } 15, 53 -- Gecko/20100101 Thunderbird/60.6.1 } 15, 53 -- MIME-Version: 1.0 } 15, 53 -- In-Reply-To: {201904012351.x31Npw2e024329-at-microscopy.com} } 15, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 15, 53 -- Content-Language: en-US } 15, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
From otiscatr45bdsk-at-gmail.com Tue Apr 2 09:36:09 2019 Return-Path: {otiscatr45bdsk-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.163.198] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x32EZwbL006778 for {microscopylistserverarchive7-at-microscopy.com} ; Tue, 2 Apr 2019 09:36:08 -0500 Received: from unknown (HELO qnx.mdrost.com) (Tue, 02 Apr 2019 10:34:37 -0400) by group21.345mail.com with ASMTP; Tue, 02 Apr 2019 10:34:37 -0400 Message-ID: {8C7D1CBF.5973475E-at-gmail.com}
Greetings Microscopists
Early bird registration for Quantitative Microanalysis 2019 has been extended to April 15th!
The deadline for Early Career Scholar financial support has also been extended to April 15th. Students who submit an abstract for a presentation or poster by April 15th with advisor support will have first priority for NSF funding reimbursement. Additional funds for students who submit an abstract or attend the meeting will be reimbursed on a funds availability basis.
Our invited speakers to date include: * Ben Buse (University of Bristol, UK): Overview of EPMA-WDS and field emission gun EPMA. * John Donovan (Probe Software, USA): Compositional mapping. * Karsten Goemann (University of Tasmania, Hobart, Australia): Fine tuning SEM for quantitative analysis and combined EDS-WDS * Mike Jercinovic (University of Massachusetts, Amherst, USA), Trace element analysis * Colin MacRae (CSIRO Mineral Resources, Clayton, Australia): Cathodoluminescence * Mike Megnason (NIST, USA): Standards based SEM-EDS analysis * Aurelien Moy (University of Wisconsin): Thin films and Fe L line measurements * William Nachlas (Syracuse University, USA): Standards and reference materials
QMA 2019 will be held at the University of Minnesota in Minneapolis, from June 24-27, 2019. This topical conference will focus on EPMA-WDS, SEM-EDS, and advances in microanalysis including cathodoluminescence, micro X-ray fluorescence, and other topics. The meeting will consist of a day of user meetings and interaction with sponsor representatives, and a three-day plenary meeting which includes tutorials and advances in microanalysis, instrumentation demos, group lunches and other networking opportunities, social mixers and a banquet.
Thanks to our sponsors! Platinum: Thermo Fisher, Oxford Instruments, Cameca, JEOL, and Bruker Gold: Probe Software Silver: EMS, Tescan, Mager Scientific, SPI, ibss Group
Abigail Lindstrom
NIST MML 643.03
100 Bureau Drive, Mailstop 8371 Gaithersburg, MD 20899
I've finished adding building instructions and such to my Lego Microscope page:
https://www.mta.ca/dmf/lego.html
including cool 3-D rotating views, links to Lego Digital Designer and the LDD files for each model. Let me know if you have any questions.
By the way, there are a number of sites that will let you order individual Lego pieces. I haven't checked all the parts, but in browsing around the two sites below I was able to find all the parts necessary for the light microscope. Prices aren't shocking, either.
https://shop.lego.com/en-CA/Pick-a-Brick
https://www.bricklink.com
Enjoy,
Jim
--
James M. Ehrman Digital Microscopy Facility Mount Allison University 63B York St. Sackville, NB E4L 1G7 CANADA
From tammyhoward072d-at-gmail.com Sat Apr 6 12:57:22 2019 Return-Path: {tammyhoward072d-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.163.209] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x36HvLXG010191 for {microscopylistserverarchive7-at-microscopy.com} ; Sat, 6 Apr 2019 12:57:21 -0500 Received: from [135.218.22.108] by qnx.mdrost.com with LOCAL; Sat, 06 Apr 2019 12:51:47 -0500 Received: from unknown (101.46.240.230) by mx03.listsystemsf.net with SMTP; Sat, 06 Apr 2019 12:37:21 -0500 Message-ID: {61323996.241C3A30-at-gmail.com}
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both chrisbrantner-at-gwu.edu, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Email: chrisbrantner-at-gwu.edu Name: Chris Brantner
Organization: George Washington University
Title-Subject: [Filtered] GWNIC CLEM Workshop June 10-14, 2019
Message: Greetings microscopists!!
I want to draw your attention to an exciting workshop that we are hosting at the George Washington Nanofabrication and Imaging Center June 10-14, 2019. We will have an exciting lineup of speakers as well as a week of light and electron microscopy demonstrations related to how we use Correlative Microscopy in our work.
Please visit the link for more information.
https://nic.gwu.edu/clem-workshop
Chris Brantner
Login Host: 161.253.36.203 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
You are warmly welcomed to submit the results of your research to the AVS66 meeting Nanometer Science and Technology Division (NSTD) sessions. NSTD explores the science and technology that emerges when material is shrunk to the nanoscale, including nanoscale devices and quantum systems exploiting nanoscale design and characterization, role of nanomaterials in novel devices and constructs, as well as surface chemistry, energetics, mechanics, and imagery.
This year, the specific emphasis will be made on the key connections between nanoscale physical and chemical phenomena induced in confined volumes as probed and manipulated by electron beams, scanning probe tips, electromagnetic radiation, ions, as well as approaches to harness these phenomena for nanoscale and atom-by-atom fabrication. The NSTD particularly promotes novel physical phenomena emerging in these nanosystems, and their applications for quantum information systems, sensing, and other applications.
The meeting features outstanding set of invited speakers in many areas of electron and scanning probe microscopy, including Ondrej Krivanek (NION) and Ilke Arslan (Argonne), Joe Stroscio (NIST), Paul Weiss (UCLA), and John Sader (U. Melbourne), and nanotechnology experts such as Chennupati Jagadish (ANU).
The detailed information about the program, graduate student and postdoctoral awards, and NSTD recognition award are available at
https://conta.cc/2TKJlnV
Looking forward to seeing you in Columbus, Ohio, on October 20-25!
Sergei
-- Sergei V. Kalinin
Director, Institute for Functional Imaging of Materials
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both peta.clode-at-uwa.edu.au, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Email: peta.clode-at-uwa.edu.au
Name: Peta Clode
Organization: University of Western Australia
Title-Subject: [Filtered] Imaging of dry ice
Message: Hi, does anyone have any experience imaging dry ice (ie. solid CO2) by electron microscopy? We are keen to visualise the microstructure (10-100s microns), but have concerns regarding changes with sublimation etc. under vacuum. Any advice appreciated!
Login Host: 130.95.124.90 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
The 7 lecture course on Scanning Probe Microscopy measurements on electronic and ionic transport on the nanometer and atomic scales is now available on the YouTube. The course includes the primers on Kelvin Probe Force Microscopy (KPFM) and its dynamics versions, KPFM on ferroelectric and grain boundaries, KPFM in liquids and environmental effects on KPFM, Scanning Gate and Scanning Capacitance microscopies, as well as IV imaging and spectroscopies on electronically, ionically, and mixed conductors. It also contains information on SPM techniques developed at the Center for Nanophase Materials Sciences (www.cnms.ornl.gov).
The course includes ~350+ slides and ~7 hours of recording, and covers topics:
Lecture 1: Transport measurements by Scanning Probe Microscopy https://youtube.com/watch?v=PjjjXij7930
Lecture 2: Introduction to Kelvin Probe Force Microscopy (KPFM) https://youtube.com/watch?v=WB0s9cwIuxM
Lecture 3: Dynamic Kelvin Probe Force Microscopy https://youtube.com/watch?v=NgQd-i77Plg
Lecture 4: Kelvin Probe Force Microscopy of Lateral Devices https://youtube.com/watch?v=-7vlVrzGTeA
Lecture 5: Kelvin Probe Force Microscopy in Liquids https://youtube.com/watch?v=yE6eMhSmhPQ
Lecture 6: Current-voltage Measurements in Scanning Probe Microscopy https://youtube.com/watch?v=HzXO0vbWy7E
Lecture 7: Dynamic IV measurements in SPM https://youtube.com/watch?v=vFgL097xTKI
Share, like, and subscribe!
Sergei
-- Sergei V. Kalinin
Director, Institute for Functional Imaging of Materials
Fellow MRS, IEEE, APS, Foresight Institute, IoP, AVS
Oak Ridge National Laboratory
Phone: (865) 241-0236
==============================Original Headers============================== 18, 90 -- From sergei2-at-ornl.gov Tue Apr 9 11:23:05 2019 18, 90 -- Received: from mta02.ornl.gov (mta02.ornl.gov [128.219.177.136]) 18, 90 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x39GN5cH031835 18, 90 -- for {microscopy-at-microscopy.com} ; Tue, 9 Apr 2019 11:23:05 -0500 18, 90 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 18, 90 -- d=ornl.gov; i=-at-ornl.gov; q=dns/txt; s=p20151116; 18, 90 -- t=1554827664; x=1586363664; 18, 90 -- h=from:to:subject:date:message-id: 18, 90 -- content-transfer-encoding:mime-version; 18, 90 -- bh=L0z81/G1VEaf2aGuM4x9KUXo8rMRyZNapFf5DDorpXU=; 18, 90 -- b=wT90cAsMvYKg6CPkHTScSa3FyQNRJZK5pFl0LK3AYuto2oRSQp9o/Amx 18, 90 -- sSHjiJo6nWxw7bcXwViLVg+DcSbZaWCn+wJKhG6dF5Ch6IShFJXZLOJLh 18, 90 -- WnpnZGUDvxsexSzY22+YOCzC1QZLWZF/cgX+HlQ0lD8JXHdfg8SVIN1Fh 18, 90 -- 5o+bnkpP++suIZWo4n0gWxdIhPqoebqktNncmaY7veLmOpRH2IqYPr8n4 18, 90 -- CxXNQdpXEAPFW3/RV4m9JbucKUCy0IL1WAMT0qWLQDustRRZDgKJek/UE 18, 90 -- 1uWWsypvyIxrD2epgYl5AiQFaova7Zv+qeXK6KhK9H41amSAr59WPAY7T 18, 90 -- w==; 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Title-Subject: [Filtered] Heat polymerizing LR White Popoffs
Message: I would like to use LR White resin to popoff a de-paraffinized tissue section from a slide and then perform immuno labeling for EM. The problem is that I have not found a good way to heat polymerize the LR White without introducing air into the BEEM capsule that is upside down on the slide. I have used gelatin capsules with LR white for embedding pieces of tissue and it has worked well but gelatin capsules will not work upside down on the slide. The resin flows out. Has anyone attempted this and had success? Login Host: 128.151.71.24 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
What an exciting challenge! I looked at a phase diagram for CO2 and I suspect you're going to need very low temperatures, very low indeed. Please keep the list informed of your progress.
Stay safe...........
Frank Karl Microscopist Akron Rubber Development Laboratory 2887 Gilchrist Road Akron, Ohio 44305
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Tuesday, April 09, 2019 8:37 AM To: Frank Karl {frank_karl-at-ardl.com}
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Email: peta.clode-at-uwa.edu.au
Name: Peta Clode
Organization: University of Western Australia
Title-Subject: [Filtered] Imaging of dry ice
Message: Hi, does anyone have any experience imaging dry ice (ie. solid CO2) by electron microscopy? We are keen to visualise the microstructure (10-100s microns), but have concerns regarding changes with sublimation etc. under vacuum. Any advice appreciated!
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-------- Forwarded Message -------- X-from: Evelyn York {eyork-at-psemi.com}
Do you have an E-SEM with a Peltier Stage??
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Tuesday, April 09, 2019 5:38 AM To: Evelyn York
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Email: peta.clode-at-uwa.edu.au
Name: Peta Clode
Organization: University of Western Australia
Title-Subject: [Filtered] Imaging of dry ice
Message: Hi, does anyone have any experience imaging dry ice (ie. solid CO2) by electron microscopy? We are keen to visualise the microstructure (10-100s microns), but have concerns regarding changes with sublimation etc. under vacuum. Any advice appreciated!
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X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu} To: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com}
Gayle,
2 thoughts occur: First, leave the gelatin capsule right-side up, overfill it enough to get a convex surface, put a thin layer of LR White on the section, just enough to infiltrate the section and exclude air from the tissue, then put the slide on the capsule upside-down (section down). Given the sizes of gelatin capsules and paraffin sections, you should be able to use 3 or 4 capsules and so balance the slide. Better, collect the sections on 22 mm square coverslips.
Second, use BEEM capsules but first coat the outside with an oxygen impermeable coating. Dip the BEEM capsule in an epoxy resin and polymerize, or perhaps (clear) fingernail polish. Etc. Overfill the BEEM capsule and mount the sections on the BEEM capsule as above, but after getting the section mounted on top of the capsule, invert the capsule so its upside-down on the section, as is usually down.
Phil ------------- Philip Oshel Imaging Facility Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 989 774-3576 office 989 774-7567 lab Email: gayle_schneider-at-urmc.rochester.edu Name: Gayle Schneider Organization: University of Rochester Title-Subject: [Filtered] Heat polymerizing LR White Popoffs Message: I would like to use LR White resin to popoff a de-paraffinized tissue section from a slide and then perform immuno labeling for EM. The problem is that I have not found a good way to heat polymerize the LR White without introducing air into the BEEM capsule that is upside down on the slide. I have used gelatin capsules with LR white for embedding pieces of tissue and it has worked well but gelatin capsules will not work upside down on the slide. The resin flows out. Has anyone attempted this and had success?
X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu} To: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com}
Do you have access to a microCT system? This might be a better bet, as you shouldn't need a vaccum.
------------- Philip Oshel Imaging Facility Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 989 774-3576 office 989 774-7567 lab
Email: peta.clode-at-uwa.edu.au Name: Peta Clode Organization: University of Western Australia Title-Subject: [Filtered] Imaging of dry ice Message: Hi, does anyone have any experience imaging dry ice (ie. solid CO2) by electron microscopy? We are keen to visualise the microstructure (10-100s microns), but have concerns regarding changes with sublimation etc. under vacuum. Any advice appreciated!
X-from: Duraine, Lita {duraine-at-bcm.edu} To: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com}
Hi Gayle,
I had to do this once and almost lost my mind....people don't laugh. Try it the other way around. Line up you capsules in a holder, fill with LR White to the top until it makes a dome shape above the the end of the capsule. Now place and lay the the slide with the tissue downward on top of the capsule. Some LR White will run down but that is Ok. Worked for us. After polymerized, carefully cut away area around capsule.
Best,
Lita Duraine
Lita Duraine Certified TEM Specialist HHMI- Molecular Genetics Duncan Neurological Research Institute Baylor College of Medicine 1250 Moursund St. Houston, TX 77030 Office: 832-824-8704 Cell: 979-549-6526 MS: N1125.50 http://flypush.imgen.bcm.tmc.edu/lab/people/lita.php
-----Original Message----- X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Wednesday, April 10, 2019 2:53 PM To: Duraine, Lita {duraine-at-bcm.edu}
-------- Forwarded Message -------- X-from: Mowery, Joseph {Joseph.Mowery-at-ARS.USDA.GOV} CC: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Hi Gayle
For me, beem capsules deform when LR-White is heat polymerized.
Surrounding the tissue with a gasket, then applying a sheet of aclar film over the top might work, afterwards the hardened resin/tissue could be glued onto a resin stub.
See this paper: “A Novel Technique for Flat-Embedding Cryofixed Plant Specimens in LR White Resin”
Best Regards,
-Joe
*Joe Mowery***| Biologist Electron and Confocal Microscopy Unit USDA Agricultural Research Service Lab 301-504-9027 | Mobile 817-821-8566 *_joseph.mowery-at-ars.usda.gov {mailto:joseph.mowery-at-ars.usda.gov} _
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Title-Subject: [Filtered] Heat polymerizing LR White Popoffs
Message: I would like to use LR White resin to popoff a de-paraffinized tissue section from a slide and then perform immuno labeling for EM. The problem is that I have not found a good way to heat polymerize the LR White without introducing air into the BEEM capsule that is upside down on the slide. I have used gelatin capsules with LR white for embedding pieces of tissue and it has worked well but gelatin capsules will not work upside down on the slide. The resin flows out. Has anyone attempted this and had success?
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Email: litaduraine-at-earthlink.net Name: Lita R Duraine
Organization: Howard Hughes Medical Institute
Title-Subject: [Filtered] Re: Heat polymerizing LR White Popoffs
Message: I had to do this once and almost lost my mind....people don't laugh. Instead of trying to place the filled capsule on top of the slide, I took the slide with the tissue attached and placed the slide over the top of an overfilled gelatin capsule filled with LR White. Line the capsules up in a holder, fill with LR White to where it forms a dome, and place the slide on top. Some will drip down but the slide should stick. Takes some doing but it works.
Best,
Lita
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I think, a SEM with a cryo stage can help. Do you have one? We have two at Monash (Clayton campus).
Good luck!
*--------------------------- * *Denis Korneev, PhD * Research Fellow School of Biological Sciences Monash University 25 Rainforest Walk, 3800 Clayton (Australia)
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Message: Hi, does anyone have any experience imaging dry ice (ie. solid CO2) by electron microscopy? We are keen to visualise the microstructure (10-100s microns), but have concerns regarding changes with sublimation etc. under vacuum. Any advice appreciated!
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From whitejoye45aymf-at-gmail.com Thu Apr 11 07:52:58 2019 Return-Path: {whitejoye45aymf-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.163.204] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x3BCqvQ2018705 for {microscopylistserverarchive7-at-microscopy.com} ; Thu, 11 Apr 2019 07:52:58 -0500 Received: from smtp.mixedthings.net ([Thu, 11 Apr 2019 02:51:59 -1000]) by mts.locks.grgtween.net with SMTP; Thu, 11 Apr 2019 02:51:59 -1000 Received: from unknown (HELO mtu67.syds.piswix.net) (Thu, 11 Apr 2019 02:41:52 -1000) by rsmail.alkoholic.net with LOCAL; Thu, 11 Apr 2019 02:41:52 -1000 Received: from public.micromail.com.au ([95.235.210.89]) by mmx09.tilkbans.com with LOCAL; Thu, 11 Apr 2019 02:27:31 -1000 Received: from [85.64.188.25] by snmp.otwaloow.com with SMTP; Thu, 11 Apr 2019 02:20:39 -1000 Received: from mx.reskind.net ([Thu, 11 Apr 2019 02:08:16 -1000]) by qrx.quickslick.com with NNFMP; Thu, 11 Apr 2019 02:08:16 -1000 Message-ID: {34F360E4.C5F0B3C4-at-gmail.com}
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On behalf of the Microscopical Society of Canada - Société de Microscopie du Canada, we would like to invite you to our annual meeting. The 2019 MSC-SMC Annual Meeting will be held between May 22nd and 24th in scenic Vancouver, British Columbia. The goals of the meeting are to bring together junior and senior researchers working in the field of imaging in Canada and abroad, to develop a strong network between imaging researchers in Canada, and to learn about exciting new research. Meeting website:
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How about a cryostage? If dry ice melts at -78, I guess you'd need quite a bit lower than that, since you only need to warm the stage to -90 to sublime off frost. The problem would be getting it into the microscope frost-free. I guess you could crack a fresh in the cryotransfer unit. Perhaps you could freeze some from liquid in a small pressure chamber - like those used for CPD, then pop the dry ice out under vacuum. I imagine the dry ice crystalline forms will vary depending on freezing conditions.
cheers, Rosemary
Dr Rosemary White CSIRO Black Mountain GPO Box 1700 Canberra, ACT 2601
T 61 2 6246 5475 M 61 468 966 713 ________________________________________ X-from: microscopy.listserver-at-gmail.com [microscopy.listserver-at-gmail.com] Sent: Thursday, 11 April 2019 10:36 p.m. To: White, Rosemary (A&F, Black Mountain)
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Do you have access to a microCT system? This might be a better bet, as you shouldn't need a vaccum.
------------- Philip Oshel Imaging Facility Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 989 774-3576 office 989 774-7567 lab
Email: peta.clode-at-uwa.edu.au Name: Peta Clode Organization: University of Western Australia Title-Subject: [Filtered] Imaging of dry ice Message: Hi, does anyone have any experience imaging dry ice (ie. solid CO2) by electron microscopy? We are keen to visualise the microstructure (10-100s microns), but have concerns regarding changes with sublimation etc. under vacuum. Any advice appreciated!
To image dry ice you will need, at the very least, a cryo stage. Since you will have the block of dry ice in roughly 1e-7 torr chamber vacuum, the vapor pressure of the dry ice should be in the 1e-9 or 1-e10 range so that evaporation doesn't occur too quickly. A quick web check indicates that the vapor pressure of CO2 is ~0.1 torr at -140C. I suspect that even at LN2 temperatures, the vapor pressure will be too high to have your sample survive long enough to look at. Do you have a liquid helium stage?
Good luck, Henk
----------------
Hendrik O. Colijn Center for Electron Microscopy and AnalysiS The Ohio State University 1305 Kinnear Rd, Suite 100
colijn.1-at-osu.edu 614/643-3458 cemas.osu.edu
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a professorship position is open at FELMI, Graz University of Technology in Austria. Please find the details here: http://tinyurl.com/yx8o3kfb
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We are pleased to announce that The Francis Crick Institute will host a two-day symposium exploring the impact of electron microscopy on biomedical research across scales, with a focus on correlative imaging. We have an exciting line-up of talks from imaging scientists at the cutting edge of correlative microscopy development and application, from macromolecules to whole organisms, and from biomedical scientists who apply these techniques to reveal new insight into health and disease. The symposium follows the 2017 Crick EM Opening Symposium, which united researchers and technology developers from the high-resolution single particle cryo-EM through to the large-volume brain imaging communities, with more than 350 attendees and a large hands-on exhibition from more than 20 commercial collaborators. Early-bird registration closes on Friday 31st May.
The speakers include:
Molecules in Cells Bridget Carragher (NYCSB, USA) Carolyn Moores (Birkbeck, UK) Tanmay Bharat (Oxford University, UK) Michael Elbaum (Weitzmann, Israel) Peter Peters (Maastricht University, The Netherlands) Gaia Pigino (MPI Dresden, Germany) Thom Sharpe (LUMC, The Netherlands)
Cells Jost Enninga (Institut Pasteur, France) Lorna Hodgson (Bristol University, UK) Jeremy Carlton (Francis Crick Institute, UK) Clare Futter (Institute of Ophthalmology, UK)
Tissues Eva Frickel (Francis Crick Institute, UK) Christian Stigloher (University of Wurzburg, Germany) Graham Knott (EPFL, Switzerland) Rachel Templin (EMBL, Germany)
New Frontiers - Correlative Imaging Jacob Hoogenboom (TU Delft, The Netherlands) Alexandra Pacaneau (ESRF, France) Martin Jones (Francis Crick Institute, UK)
New Frontiers - Modelling and Image Analysis Katie Bentley (Francis Crick Institute, UK) Anna Kreshuk (EMBL, Germany) Stephan Preibisch (MDC Berlin, Germany) The Future of Correlative Imaging Andreas Walther (BioImaging Austria)
The symposium will take place on Monday 15th and Tuesday 16th July 2019. Talks will take place from 10am to 6pm each day, and there will be a symposium dinner on the Monday, and a drinks reception on the Tuesday. Registration can be found at the Royal Microscopical Society website: https://www.rms.org.uk/discover-engage/event-calendar/crick-em-symposium-2019.html
Advanced CLEM Course: Wednesday 17th – Friday 19th July 2019
The symposium will be followed by a three-day advanced CLEM course from Wednesday 17th to Friday 19th July 2019. This will involve a series of hands-on workshops, demos and tutorials in the comprehensive EM facilities at the Crick, run by experts from the Crick and our academic and commercial partners. The course will cover 3D correlative light and electron microscopy, integrated light and electron microscopy, cryo-correlative light and electron microscopy, and software solutions for correlative imaging. Further details of the course can be found here:
https://www.rms.org.uk/discover-engage/event-calendar/clem-course-2019.html We look forward to welcoming you to the Crick in July!
The Organisers (Lucy Collinson, Raffa Carzaniga, Paul Verkade, Yannick Schwab)
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Title-Subject: [Filtered] Service Manual for a Hitachi S4700 FESEM
Message: We are trying to do some diagnoses with the assistance of an off-site service engineer, but we are lacking a copy of the service manual for our S4700 FESEM
Is anybody willing to share a copy, preferably by .pdf?
If so, please contact me directly.
Thank you in advance.
-Derrick
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1. We have recently explored a way of publishing a scientific paper together with a code used for the analysis as one document. The idea is to augment a classical paper organization with code cells that appear hidden by default (when viewed in Colab). This should allow a reader to retrace analysis and, more importantly, enables the readers to use the same codes for their data or clone and modify the codes. We hope that, in the interest of reproducibility in science, this can become one of standard form of publication in the future.
Please check out the result of this effort here: https://doi.org/10.26434/chemrxiv.8001473.v1.
(use 'Open in Colab' icon to access an interactive paper mode; notice that currently this is the only way to get a proper text/code rendering)
2. The collection of YouTube lectures on Piezoresponse Force Microscopy and Spectroscopy and Kelvin Probe Force Microscopy are now moved to the dedicated YouTube channel M*N: Microscopy, Machine Learning, Materials. We will start to deposit lectures on machine learning applications in scanning transmission electron microscopy, scanning probe microscopy shortly - subscribe!
3. As a reminder, we have recently expanded the PyCroscopy package for imaging data analysis to include workflows for deep/machine learning analysis of atomically-resolved data. The new repository is called AI Crystallographer (https://github.com/pycroscopy/AICrystallographer) and it includes interactive Google Colab & Jupyter notebooks with the detailed description of how to run the analysis, as well as pre-trained models for certain type of atomic systems. AI Crystallographer is an active project and we expect to be adding more workflows and pre-trained models in the near future.
The core PyCroscopy package, developed and maintained by Suhas Somnath, Rama Vasudevan and others, is an open source package for storing, visualizing, processing, and analyzing scientific imaging / microscopy data. PyCroscopy takes a data-centric approach, wherein the raw data acquired from the instrument, processed, analyzed, and intermediate results are formatted according to Universal Spectroscopy and Imaging Data (USID) and stored in a single HDF5 file. USID's generalized structure, has facilitated the development and deployment of a variety of instrument-agnostic algorithms available in Pycroscopy.
The AI Crystallographer extension of PyCroscopy aims at bringing the recent developments in the fields of machine learning and computer vision to the microscopy and microanalysis community, with a specific emphasis on the analysis of atomically-resolved data. Currently it includes the following sub-packages:
- DefectNet: Complete workflow for locating atomic defects in electron microscopy movies with a convolutional neural network using only a single movie frame to generate a training set. It is based on paper in npj Computational Materials 5, 12 (2019), but now with the updated augmentation procedure (includes adding noise, zoom-in and flip/rotations) and using PyTorch deep learning framework instead of the Keras one for model training/predictions.
- AtomNet: Application of a fully convolutional neural network for locating atoms in noisy experimental scanning transmission electron microscopy data from graphene. Based on paper in ACS Nano 11, 12742 (2017), but now with a better model (gives "cleaner" predictions) and using PyTorch instead of Keras. The current model is limited to graphene lattice, but we expect to upload more models for different systems in the near future.
- FerroNet: Application of different machine learning and multivariate analysis tools (neural networks, dimensionality reduction, clustering/unmixing) for analysis of ferroic distortions in high-resolution scanning transmission electron microscopy data on perovskites. Based on (soon to be submitted) work.
- SymmetryNet: Application of a deep convolutional network used to determine 2D Bravais lattice symmetry from atomically resolved images. Based on paper in npj Computational Materials 4, 30 (2018).
- Tutorials: Tutorial-like notebooks on using class activation maps for locating defects in the images and using a fully convolutional neural network for cleaning atom-resolved data and locating atoms in it.
We recommend running the notebooks (files with .ipynb extension) in Google Colab, which is a Jupyter notebook environment for machine learning research that requires no setup to use and also provides free GPU/TPU (just click on "open in Colab" icon in a notebook). You should be able to execute the current workflows with your data or perhaps modify/adjust/customize them specifically for your systems (e.g. the current AtomNet is limited to graphene but the tutorial that we provided shows how to train this type of models in general).
If you have any questions or want to report that something is broken (smiley face), the best way to contact us is by filing an issue in AICrystallography GitHub repository.
If interest in using and participating in the development of the AI tools for STEM data analysis, please contact the AI Crystallographer team:
You can find multiple examples of AI Crystallographer applications for real materials systems in our publications (either classical journal, or on arxiv)
From thivisid08uu-at-gmail.com Fri Apr 19 20:14:32 2019 Return-Path: {thivisid08uu-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.163.206] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x3K1EQ6L001348 for {microscopylistserverarchive7-at-microscopy.com} ; Fri, 19 Apr 2019 20:14:31 -0500 Received: from [56.45.41.189] by external.newsubdomain.com with NNFMP; Fri, 19 Apr 2019 17:02:05 -0800 Received: from relay37.vosimerkam.net ([20.203.99.41]) by mmx09.tilkbans.com with LOCAL; Fri, 19 Apr 2019 16:59:49 -0800 Message-ID: {C6F9EEF4.BE024072-at-gmail.com}
Dear All,
I am looking for a Bruker XFlash Powersupply and Processing Unit for the XFlash 5030 EDS detector.
If there is a spare one somewhere please contact me.
Thanks,
Stefan
--
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
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Title-Subject: [Filtered] Life Sciences Product Specialist
Message: Live and work in one of the most beautiful areas in northern California – Redding, CA. Ted Pella Inc. is seeking a Life Sciences Product Specialist who will be responsible product development and sales and marketing activities related to TEM and LM life science related products, and related supplies for other types of microscopy. Help us grow and develop these product lines.
We are looking for someone with BS in Life Sciences or related, 5 years hands on TEM instrument use and specimen prep experience, 5 years of LM instrument use and specimen prep experience. To apply email cover letter, resume and salary requirements to human_resources-at-tedpella.com. See our full job posting at www.tedpella.com Login Host: 12.7.209.242 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
From normanatlas54ji-at-gmail.com Tue Apr 23 23:15:51 2019 Return-Path: {normanatlas54ji-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.163.201] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x3O4Fm3E014347 for {microscopylistserverarchive7-at-microscopy.com} ; Tue, 23 Apr 2019 23:15:49 -0500 Received: from [107.46.206.30] by m1.gns.snv.thisdomainl.com with ESMTP; Tue, 23 Apr 2019 21:53:55 -0600 Message-ID: {8c9101d4fa1c$d83aafa0$3a1a90d7-at-normanatlas54ji}
X-from: Andrew_Morgan-at-bellsouth.net
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Email: Andrew_Morgan-at-bellsouth.net
Name: Andrew Morgan
Organization: Equipment Semi
Title-Subject: [Filtered] TEM - FEI Tecni F20 TEM Tilt Holder
Message: Hello,
I am looking for a double tilt holder (hex ring style) for a FEI Tecni F20 TEM. Name & PN is: CompuStage Low-Background, Double-Tilt Part# FP 6595/15
We’re looking for a used part (as pricing for new is too steep) and the need is immediate. Does anyone happen to have one available or know where I can find one? Thanks in advance for any assistance you can provide.
Thank you, Andrew Morgan
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From andralbi54oary-at-gmail.com Thu Apr 25 14:58:03 2019 Return-Path: {andralbi54oary-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.163.199] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x3PJw1Sg021359 for {microscopylistserverarchive7-at-microscopy.com} ; Thu, 25 Apr 2019 14:58:02 -0500 Received: from [118.2.119.117] by m1.gns.snv.thisdomainl.com with LOCAL; Fri, 26 Apr 2019 03:44:45 +0800 Received: from mtu23.bigping.com [174.17.249.148] by external.newsubdomain.com with ESMTP; Fri, 26 Apr 2019 03:28:47 +0800 Received: from [213.96.189.156] by mailout.endmonthnow.com with NNFMP; Fri, 26 Apr 2019 03:25:05 +0800 Message-ID: {CE64EA17.BB4F4839-at-gmail.com}
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Message: Pathcore is a Toronto-based software company specializing in data management and workflow software for digital pathology. We offer a browser-based platform for managing, viewing and annotating pathology images as well as all associated metadata and other digital content.
We’re looking for a results-driven Sales Manager to actively seek out and engage new customer prospects in a defined territory within the US and Canada. The successful candidate will have a proven track record of building relationships in the digital pathology space, or related fields, and will able to demonstrate a successful career in sales and/or business development. Apply directly: www.pathcore.com/careers
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QMA 2019 focuses on quantitative microanalysis using electron-probe microanalysis and scanning electron microscopy, with an educational focus on microanalysis. QMA 2019 will be held June 24-27, 2019 at the University of Minnesota, Minneapolis, MN, USA. The conference will be a plenary meeting with user meetings on Monday June 24, followed by three days of technical talks, and will include tutorials on microanalysis by EPMA-WDS and SEM-EDS, invited talks by leaders in the field of microanalysis, group discussion, laboratory remote demonstrations, poster sessions, and group meals.
*Early Career Scholars will receive a maximum of $750 in reimbursement for travel, lodging, and registration for QMA 2019.* No abstract or presentation is required. Funds are available on a first come, first serve basis. Priority is given to students who have a letter of support from their advisor or employer.
The details of the ECS support program are as follows:
- Eligible ECS include undergraduate, graduate, post-doctoral, and early career professional scientists who are within two years of their last degree. - The ECS support program is for USA citizens at domestic institutions. Foreign nationals at domestic institutions may be supported subject to additional requirements. - ECS candidates should be identified and proposed to us by their sponsor. An email letter of support is required which includes confirmation that the ECS candidate is a student. We will give priority to those candidates having co-sponsorship financial support from their advisor or sponsoring agency. - Consideration for those without sponsorship and who have a financial need will be made based on available funding. - ECS awardees will receive a maximum of $750 in reimbursement for expenses which may include travel, lodging, and registration for QMA 2019. Receipts must be submitted at QMA 2019 for reimbursement after the conference. - The QMA 2019 registration for ECS is $100 and will be paid by the ECS attendee.
ECS awardees will stay at the University of Minnesota campus dormitory.
Our young scientist community is very important, and we want to enable ECS candidates to attend QMA 2019. Please contact Heather Lowers (hlowers-at-usgs.gov {mailto:hlowers-at-usgs.gov} ) or Paul Carpenter (paul.carpenter.epma-at-gmail.com {mailto:paul.carpenter.epma-at-gmail.com} ) for additional information.
Full details on the QMA 2019 TC can be found on the MAS website: QMA 2019 web page: https://the-mas.org/events/topical-conferences/qma-2019/
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Title-Subject: [Filtered] - Advanced Correlative Light and Electron Microscopy (CLEM) Course 2019. The Francis Crick Institute - London (UK)
Message: Dear Colleagues ,
The deadline is approaching for this course: Advanced Correlative Light and Electron Microscopy (CLEM) Course 2019 - Wednesday 17th – Friday 19th July 2019
This will involve a series of hands-on workshops, demos and tutorials in the comprehensive EM facilities at The Francis Crick Institute, run by experts from the Crick and our academic and commercial partners. The course will cover these workflows: 3D Correlative Light and Electron Microscopy Integrated Light and Electron Microscopy Cryo-Correlative Light and Soft X-Ray tomography Software solutions for correlative imaging Deadline for applications: Tuesday, 30th April 2019. Acknowledgment of successful applicants: Monday 13th May 2019. Course Fee: Ł150
Further details of the course can be found here: https://www.rms.org.uk/discover-engage/event-calendar/clem-course-2019.html Best wishes, Raffa Carzaniga and Lucy Collinson, https://www.crick.ac.uk/research/platforms-and-facilities/electron-microscopy
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From westelle638yyyr-at-gmail.com Mon Apr 29 15:06:07 2019 Return-Path: {westelle638yyyr-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.163.206] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x3TK659f006984 for {microscopylistserverarchive7-at-microscopy.com} ; Mon, 29 Apr 2019 15:06:06 -0500 Received: from rly04.hottestmile.com ([Tue, 30 Apr 2019 01:51:12 +0600]) by smtp.endend.nl with ESMTP; Tue, 30 Apr 2019 01:51:12 +0600 Received: from smtp4.cyberemailings.com [211.85.184.39] by mail.gimmicc.net with SMTP; Tue, 30 Apr 2019 01:41:21 +0600 Received: from webmail.halftomorrow.com [26.79.112.173] by smtp.mixedthings.net with SMTP; Tue, 30 Apr 2019 01:33:55 +0600 Received: from m1.gns.snv.thisdomainl.com ([Tue, 30 Apr 2019 01:29:10 +0600]) by mail.gimmicc.net with QMQP; Tue, 30 Apr 2019 01:29:10 +0600 Received: from nntp.pinxodet.net [176.102.9.131] by asx121.turbo-inline.com with ESMTP; Tue, 30 Apr 2019 01:10:33 +0600 Message-ID: {D11CCC0E.57929F8D-at-gmail.com}
Hello Everyone,
Just letting the community know that we have a temporary 6-month position available here at the University of British Columbia for a general biological TEM imaging tech. This would be a great entry-level position for anyone looking to add to their resume/CV. The candidate would be responsible for running and preparing samples for our Hitachi H7600 120kV TEM, as well as our FEI Tecnai G2 200kV LaB6 TEM. In addition to the standard TEM sample prep equipment, we also have a Leica HPM100 High Pressure Freezer, an AFS2 freeze-substitution machine, an FEI Vitrobot MkIV, and an IGL automatic immunogold labeling system. We also have a dedicated image processing PC with Amira software and a Wacom Cintiq 24 pen display to assist with segmentation of various types of data.
Our Tecnai TEM is used for TEM Tomography, Cryo TEM, and HRTEM in addition to standard imaging, so there are also opportunities to work with some more advanced TEM imaging and sample prep techniques in this position.
For more information go to https://www.hr.ubc.ca/careers-postings/staff-s.php and search for Job ID 33611.
Thanks, Bradford Ross
Electron Microscopy Technician BioImaging Facility University of British Columbia Cunningham Building Rm. 64 2146 East Mall Vancouver, B.C. V6T 1Z4
phone 604-822-6996
==============================Original Headers============================== 8, 46 -- From bradford.ross-at-botany.ubc.ca Tue Apr 30 11:45:20 2019 8, 46 -- Received: from vmaprod5.mail-relay.ubc.ca (vmaprod5.mail-relay.ubc.ca [142.103.117.139]) 8, 46 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x3UGjKYk010850 8, 46 -- for {microscopy-at-microscopy.com} ; Tue, 30 Apr 2019 11:45:20 -0500 8, 46 -- Received: from vmaprod5.mail-relay.ubc.ca (localhost.localdomain [127.0.0.1]) 8, 46 -- by localhost (Email Security Appliance) with SMTP id 044C52D422F_CC87B1BB 8, 46 -- for {microscopy-at-microscopy.com} ; Tue, 30 Apr 2019 16:43:07 +0000 (GMT) 8, 46 -- Received: from mx2.mail-relay.ubc.ca (lbpglfsc01gstp01-ents01-f5-vrfglue-float.systems.it.ubc.ca [10.45.24.97]) 8, 46 -- (using TLSv1.2 with cipher ECDHE-RSA-AES256-GCM-SHA384 (256/256 bits)) 8, 46 -- (Client CN "mx2.mail-relay.ubc.ca", Issuer "mx2.mail-relay.ubc.ca" (not verified)) 8, 46 -- by vmaprod5.mail-relay.ubc.ca (Sophos Email Appliance) with ESMTPS id AC0112D15C6_CC87B1AF 8, 46 -- for {microscopy-at-microscopy.com} ; Tue, 30 Apr 2019 16:43:06 +0000 (GMT) 8, 46 -- Received: from smtp.mail.ubc.ca (s-itsv-hub04p.ead.ubc.ca [137.82.151.86]) 8, 46 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 8, 46 -- (Client CN "rpc.mail.ubc.ca", Issuer "Entrust Certification Authority - L1K" (not verified)) 8, 46 -- by mx2.mail-relay.ubc.ca (Postfix) with ESMTPS id 9B11115F598 8, 46 -- for {microscopy-at-microscopy.com} ; Tue, 30 Apr 2019 09:43:06 -0700 (PDT) 8, 46 -- Received: from EXCH-SRV03BP.ead.ubc.ca (10.19.216.45) by 8, 46 -- s-itsv-hub04p.ead.ubc.ca (137.82.151.86) with Microsoft SMTP Server (TLS) id 8, 46 -- 14.3.408.0; Tue, 30 Apr 2019 09:43:05 -0700 8, 46 -- Received: from EXCH-SRV03AP.ead.ubc.ca (10.19.216.43) by 8, 46 -- EXCH-SRV03BP.ead.ubc.ca (10.19.216.45) with Microsoft SMTP Server 8, 46 -- (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_128_GCM_SHA256) id 8, 46 -- 15.1.1713.5; Tue, 30 Apr 2019 09:43:05 -0700 8, 46 -- Received: from EXCH-SRV03AP.ead.ubc.ca ([fe80::a54d:1db5:afc5:1df7]) by 8, 46 -- EXCH-SRV03AP.ead.ubc.ca ([fe80::a54d:1db5:afc5:1df7%7]) with mapi id 8, 46 -- 15.01.1713.004; Tue, 30 Apr 2019 09:43:05 -0700 8, 46 -- From: "Ross, Bradford" {bradford.ross-at-botany.ubc.ca} 8, 46 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 8, 46 -- Subject: TEM Tech Position Available 8, 46 -- Thread-Topic: TEM Tech Position Available 8, 46 -- Thread-Index: AQHU/3L/2s2SriH+2Ua9mUSyTNGiYw== 8, 46 -- Date: Tue, 30 Apr 2019 16:43:04 +0000 8, 46 -- Message-ID: {509412cc68aa4d1aa58920d757c037bf-at-botany.ubc.ca} 8, 46 -- Accept-Language: en-US, en-CA 8, 46 -- Content-Language: en-US 8, 46 -- X-MS-Has-Attach: 8, 46 -- X-MS-TNEF-Correlator: 8, 46 -- x-originating-ip: [137.82.85.241] 8, 46 -- x-pmwin-version: 4.0.4, Antivirus-Engine: 3.74.1, Antivirus-Data: 5.62 8, 46 -- Content-Type: text/plain; charset="iso-8859-1" 8, 46 -- MIME-Version: 1.0 8, 46 -- X-PMWin-Version: 3.1.3.0, Antivirus-Engine: 3.74.1, Antivirus-Data: 5.62 8, 46 -- X-SASI-RCODE: 200 8, 46 -- Content-Transfer-Encoding: 8bit 8, 46 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id x3UGjKYk010850 ==============================End of - Headers==============================
We have an open position for an SEM Facility Manager here in the NUANCE Center at Northwestern University.
The SEM Facility Manager is responsible for all aspects of the SEM/FIB Facility. This includes: equipment purchasing, upgrades and maintenance; supervision of Microscopy and Imaging Specialist; user training and technical support on all SEM instruments and related equipment; course development and teaching of laboratory components of SEM related courses; development of new analytical techniques and capabilities; providing analytical services for both internal NU and external industrial clients; conducting facility tours and demonstrations. In addition, this position has primary technical responsibility for the dual-beam Focused Ion Beam (FIB) instrument and assists in sample preparation for all electron microscopy, including Transmission Electron Microscopy (TEM).
MS/PhD, 4-5+ years hands-on characterization experience (post-degree) and 2+ years facility management/user training experience preferred.
More info here: http://www.nuance.northwestern.edu/docs/JOBs_docs/sem-facility-manager-2019.pdf
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Email: nmz787-at-gmail.com Name: Nathan T McCorkle
Message: Does anyone have any info on the technical specs/details of these units? I'm seeing some on eBay for what looks to be an OK price, but they seem a lot smaller than other items I've been seeing. What I mean is, the tube hanging off the black box is only about an inch before hitting a Tee... while many of the RGAs I see have a plate assembly attached there which is probably 4 to 6 inches long (in a vacuum pipe). Being new to RGAs, I wonder if maybe those longer assemblies are ionizers or some sort of amplifier (electron multiplier) that this TR100 lacks, or possibly the TR100 is simply newer and the tech got smaller?
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both michael.cammer-at-med.nyu.edu, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Email: michael.cammer-at-med.nyu.edu Name: Michael Cammer
Organization: NYU Langone
Title-Subject: [Filtered] 8 bit vs 16 bit images in all microscopy
Message: I am curious how people package digital data for themselves and other users. A few months ago on the confocal listserv we had a discussion regarding the sanctity of light microscopy data. One of the issues discussed was how to represent 12 to 16 bit data in an eight bit space.
Question: how do people routinely compress data into RGB for display and archiving and when is it permissible to not preserve the raw 12 to 16 bit raw data?
I have a similar question for TEM data. The new cameras on TEMs result in 16 bit images. However, for stained material, there cannot be real intensity information needing more than a few bits (or am I wrong about this?). When reducing bit depth, what is the best algorithm? Most reduction to 8 bits that I've seen involve putting the bottom x% into 0, for instance bottom 0.3% of pixel values are assigned to black. Does this risk losing the ability to see fine structure, or is this ok? Would it be preferable to not clip in the darks or is this ok? Is it ok to save only the 8 bit data and not bother with the 16 bit data? For instance, most people may not be prepared to deal with 16 bit data or consider it inconvenient.
Would very much like to know what is common practice and considered acceptable for both optical and electron microscopy images, both biological and material sciences.
Best regards- Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY 10016 Michael.Cammer-at-med.nyu.edu http://nyulmc.org/micros http://microscopynotes.com/ Voice direct only, no text or messages: 1-914-309-3270 and 1-646-501-0567
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This is an excellent discussion topic, and I can offer a practical perspective based on some time spent working with a variety of users in a shared instrument facility.
1) The ease of dealing with 16 bit images depends strongly on what camera and software packages you are using. Gatan hardware and Digital Micrograph work fairly seamlessly with 16bit images (Ultrascan and above lines), and it's easy to down-convert to 8bit TIFFs. So if your users spend most of their time within the Digital Micrograph environment, then there's no real reason to use anything but the highest bit depth offered, which is the default saving option within DM.
2) For other camera manufacturers, I've noticed that saving data in 16bit format TIFFs cause some issue with Windows (and Mac?) not being able to interpret the bit depth correctly, resulting in images being displayed as nothing but a flat gray frame in the file explorer view. This can obviously cause some frustration and confusion with users, especially corporate customers. For these cameras/software packages, I usually direct users to save their data in 8bit format, which Windows (and Mac?) are able to correctly display.
3) It also depends on what the users are planning on doing with the data. If a user is doing quantitative analysis of their image, for example correlating intensity at an atom site with number of atoms in that column or measuring sample roughness by looking at raw intensity values at neighboring atomic columns, then they would obviously need the maximum bit depth offered by the camera. Likewise, diffraction experiments benefit from as much dynamic range--and bit depth--as possible, so 16 bit is the way to go. On the other hand, if a user is simply using the microscope to image morphology or measure particle sizes, for example, then the bit depth of the images doesn't matter much. Yes, information is forever lost when saving in 8 bit, as it must be when going from 65k gray scale values to 256, but for most applications I would bet that a typical user could not pick which image is at what bit depth.
Having said all that, I know that most common image handlers, like ImageJ, will handle most anything you throw at them. I'm a bit of a data hoarder, and with most universities offering unlimited google drive storage, I find that saving in both 16bit and 8bit gives me my cake and let's me eat it too (not at the microscope console, though!). If you don't have unlimited Google Drive storage, you can find 10TB hard drives on sale for US$160 every other month on Amazon or Best Buy. That's a lot of storage!
Thanks for starting this interesting discussion. Looking forward to reading other thoughts on this matter.
Cheers, Chris
On Wed, May 1, 2019 at 10:03 PM {microscopy.listserver-at-gmail.com} wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy to both } michael.cammer-at-med.nyu.edu, Microscopy-at-Microscopy.com so that all Microscopy Listserver } Subscribers can benefit from our collective wisdom } --------------------------------------------------------------------------- } } Email: michael.cammer-at-med.nyu.edu Name: Michael Cammer } } Organization: NYU Langone } } Title-Subject: [Filtered] 8 bit vs 16 bit images in all microscopy } } Message: I am curious how people package digital data for themselves and other users. } A few months ago on the confocal listserv we had a discussion regarding the sanctity of light } microscopy data. One of the issues discussed was how to represent 12 to 16 bit data in an eight bit } space. } } Question: how do people routinely compress data into RGB for display and archiving and when is it } permissible to not preserve the raw 12 to 16 bit raw data? } } I have a similar question for TEM data. The new cameras on TEMs result in 16 bit images. However, } for stained material, there cannot be real intensity information needing more than a few bits (or am } I wrong about this?). When reducing bit depth, what is the best algorithm? Most reduction to 8 } bits that I've seen involve putting the bottom x% into 0, for instance bottom 0.3% of pixel values } are assigned to black. Does this risk losing the ability to see fine structure, or is this ok? } Would it be preferable to not clip in the darks or is this ok? Is it ok to save only the 8 bit data } and not bother with the 16 bit data? For instance, most people may not be prepared to deal with 16 } bit data or consider it inconvenient. } } Would very much like to know what is common practice and considered acceptable for both optical and } electron microscopy images, both biological and material sciences. } } Best regards- } Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory } NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY 10016 } Michael.Cammer-at-med.nyu.edu http://nyulmc.org/micros http://microscopynotes.com/ Voice direct } only, no text or messages: 1-914-309-3270 and 1-646-501-0567 } } } } } Login Host: 100.37.243.135 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 15, 53 -- From microscopy.listserver-at-gmail.com Wed May 1 21:04:23 2019 } 15, 53 -- Received: from mail-it1-f182.google.com (mail-it1-f182.google.com [209.85.166.182]) } 15, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x4224Npg018311 } 15, 53 -- for {microscopy-at-microscopy.com} ; Wed, 1 May 2019 21:04:23 -0500 } 15, 53 -- Received: by mail-it1-f182.google.com with SMTP id q14so754677itk.0 } 15, 53 -- for {microscopy-at-microscopy.com} ; Wed, 01 May 2019 19:02:15 -0700 (PDT) } 15, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 15, 53 -- d=gmail.com; s=20161025; } 15, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 15, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 15, 53 -- bh=VrZzpA6NJ61+sgD7nstJuh2vL3o+ZWHQB6LS3wuLohg=; } 15, 53 -- b=Dj5ayqh7nvxlWYOrvY3vSPcEg2qIKAOuTWdRp5nU1wjpmBKD5ZV5gs2/nnE2lMtP5K } 15, 53 -- W400rj6Y7iaTDaR72yLf/cEWyHqCqoj17GLhLAsmK9HIzX79iXiS7fcGWOjKmBKjv28r } 15, 53 -- CmBQNyl1dFZKE+SxjZQtcyr8fTy/pxuA6muV74EUAwqD56cNpYoAgG6+VRpTp8VMJDo9 } 15, 53 -- g9T/K8CZngfznHwr/Cnliekz7n/dJ2PSXX5PTbX7j32oqnazgrDEGyWk7B7c39kpCHvl } 15, 53 -- wHEWa3I4yfG5PuwUdds0a2jM/5xBvyByPjVIVatxxmRC5Yx+Q5seAUit6f9kdDEKjnRJ } 15, 53 -- hJFg== } 15, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 15, 53 -- d=1e100.net; s=20161025; } 15, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 15, 53 -- :user-agent:mime-version:in-reply-to:content-language } 15, 53 -- :content-transfer-encoding; } 15, 53 -- bh=VrZzpA6NJ61+sgD7nstJuh2vL3o+ZWHQB6LS3wuLohg=; } 15, 53 -- b=XcNNH8/Do+x1aicvHZkFKgTTwLZ2gv6JRT1CKNU9Zf3Up/GpYD1QlCfoi1g2UeKiGr } 15, 53 -- ur9sIeCk1fZXU8VQ0pFckcFHe2OFbyNbsG9WWa3I5N6NtpjFDocOGHrsY799ape4t3di } 15, 53 -- zVJ/J3Ue5PObQ1VI+LKwfCczN00aRFDmCrryfH6tO9y5ywvbj4XH6n20fChc+MjBwVvq } 15, 53 -- bL9YFWYJerm4NCE8Jh/9bah+gdiQvzSGPxznd+NwAnEWLokYjcJFTycIDsa7PWOcvC6e } 15, 53 -- NIZYp9vn3CyZKnX4C0kaEBEienY2iCHRKP2H0cJqWHEGPfp1m4lcwuruq3qga+db0TAU } 15, 53 -- Ihog== } 15, 53 -- X-Gm-Message-State: APjAAAURO4B/n6HDn8HTN1nd72IXYBMcCCbcPQm1DiyaftwUSmayGRRf } 15, 53 -- tLhEbyareXaIECrNX+IFPYtZRDxT } 15, 53 -- X-Google-Smtp-Source: APXvYqx57uatwc4Q+1QPb9o4IYj7yFHto3oBjP6le7km9U9MkOaE3+mjtXUrH9CslnixAWVxq8mX1A== } 15, 53 -- X-Received: by 2002:a02:29c4:: with SMTP id p187mr740149jap.7.1556762533488; } 15, 53 -- Wed, 01 May 2019 19:02:13 -0700 (PDT) } 15, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:75e9:97ad:65a:ef97]) } 15, 53 -- by smtp.googlemail.com with ESMTPSA id t196sm4063120ita.4.2019.05.01.19.02.12 } 15, 53 -- for {microscopy-at-microscopy.com} } 15, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 15, 53 -- Wed, 01 May 2019 19:02:13 -0700 (PDT) } 15, 53 -- Subject: viaWWW:8 bit vs 16 bit images in all microscopy } 15, 53 -- References: {201905020019.x420Jsfh014187-at-microscopy.com} } 15, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 15, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 15, 53 -- X-Forwarded-Message-Id: {201905020019.x420Jsfh014187-at-microscopy.com} } 15, 53 -- Message-ID: {5bfe63d3-44b0-769c-dc83-5d893b1c1169-at-gmail.com} } 15, 53 -- Date: Wed, 1 May 2019 21:02:12 -0500 } 15, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.14; rv:60.0) } 15, 53 -- Gecko/20100101 Thunderbird/60.6.1 } 15, 53 -- MIME-Version: 1.0 } 15, 53 -- In-Reply-To: {201905020019.x420Jsfh014187-at-microscopy.com} } 15, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 15, 53 -- Content-Language: en-US } 15, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
==============================Original Headers============================== 10, 41 -- From microwink-at-gmail.com Wed May 1 21:33:14 2019 10, 41 -- Received: from mail-io1-f65.google.com (mail-io1-f65.google.com [209.85.166.65]) 10, 41 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x422XEf4030236 10, 41 -- for {Microscopy-at-microscopy.com} ; Wed, 1 May 2019 21:33:14 -0500 10, 41 -- Received: by mail-io1-f65.google.com with SMTP id h26so754155ioj.1 10, 41 -- for {Microscopy-at-microscopy.com} ; Wed, 01 May 2019 19:31:06 -0700 (PDT) 10, 41 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 10, 41 -- d=gmail.com; s=20161025; 10, 41 -- h=mime-version:references:in-reply-to:from:date:message-id:subject:to; 10, 41 -- bh=VmLW+3tY0l9uMfBx0ljLTD6j4tLW3+mofzij8PiEJms=; 10, 41 -- b=O512HWKkt4dsLIODRNbNxb5hH9oh2zgcJ+mvEPrUEOOAWSgxjJbWlF65y45Kc6e329 10, 41 -- HqIEmTcgk493xrFN3wM5d8CDw0lgD4MSpUJLG85/+vZrGfWde5I1D3rmFZ3wXlePheYJ 10, 41 -- 14ORgaXf+CU7NHFk/0AR/hNn++z+WXt8+W0HHRNtv21ufIB/ug4rAztmE5FDL/ki616P 10, 41 -- 6MNUO9kL++EW29nIye2xHjBBQCeCWqM0oyNc4zzeZOPidVuT+om6WtrcUKh6U6s6F3qQ 10, 41 -- H38FEcQNTOt+it/yDaJ5ATTyjoob+pfuG19qQHkctdnVu0SN12FH9wrIbmgAG+EL0bqx 10, 41 -- FJVg== 10, 41 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 10, 41 -- d=1e100.net; s=20161025; 10, 41 -- h=x-gm-message-state:mime-version:references:in-reply-to:from:date 10, 41 -- :message-id:subject:to; 10, 41 -- bh=VmLW+3tY0l9uMfBx0ljLTD6j4tLW3+mofzij8PiEJms=; 10, 41 -- b=srQ501noL+bavQksLJ+lLTG12wyE/cAcvqQB90j+z3xEDBNQfir3DpGja7nvuwVrYe 10, 41 -- 3GxipDR/flekf9F6h5ZLwDE65ky8AbS4KfGLSWwYXbPBM34Cv/jg5U4WS1xFOdUH4Z06 10, 41 -- mQ1XXlKrtszDgwbvlw3PGYusTIStT9G4aXpmJqo3rsDsbiZLKnZJk9hTvWu4wMQyfBr/ 10, 41 -- vrBCRIQGGGlg6ut7c4vo5hCSWv/meADXLEe7NMhWsog+7Bbw6x9GbAY/3TdABsQd/uG0 10, 41 -- 7nkwBEmIFz2OUHpKG5CafPczic1p1b/ZrhTyNlUWM/giPbbgTvi0TtLMx8Tr/cQWTVEW 10, 41 -- thFQ== 10, 41 -- X-Gm-Message-State: APjAAAXfaBn++eL7lDq1p71hKQag1ijGdXPm1Khc9WK0dfOZMY96av9A 10, 41 -- e+TzY1UAQHZ25Z0oEoIojCoy58dYh7rmdVBX0ITsgw== 10, 41 -- X-Google-Smtp-Source: APXvYqxqkjkLTO+D/6fWWKIZI4nOc16HK/7PLwPqqeW1bIXLgpPUEdBwnnM2g8gV70j7FfPyo7/pt8CFDrlOxEfbPcs= 10, 41 -- X-Received: by 2002:a5d:8e19:: with SMTP id e25mr776726iod.139.1556764265475; 10, 41 -- Wed, 01 May 2019 19:31:05 -0700 (PDT) 10, 41 -- MIME-Version: 1.0 10, 41 -- References: {201905020205.x4225rHH019712-at-microscopy.com} 10, 41 -- In-Reply-To: {201905020205.x4225rHH019712-at-microscopy.com} 10, 41 -- From: Christopher Winkler {microwink-at-gmail.com} 10, 41 -- Date: Wed, 1 May 2019 22:30:53 -0400 10, 41 -- Message-ID: {CAA8T2PNTedZCCH=GtKqiOGMDufqOD1F_ozp+9YJS-sJNTznz7A-at-mail.gmail.com} 10, 41 -- Subject: Re: [Microscopy] viaWWW:8 bit vs 16 bit images in all microscopy 10, 41 -- To: Microscopy-at-microscopy.com 10, 41 -- Content-Type: text/plain; charset="UTF-8" ==============================End of - Headers==============================
} } } 02.05.2019 at 04:06: } I have a similar question for TEM data. The new cameras on TEMs result in 16 bit images.
this is not new, at all. 1st, after analog recording on negatives, and later digitization, I cannot remember to have stored 8bit data only, back in my early times in the 1980's. I have ALWAYS stored all data in 16bit mode. The first camera we bought in the 1990's (it was a used one), a TVIPS 1k x 1k camera, already recorded data with 12bit depth and stored them as 16bit files. These files could easily be handled in the 1990's by either ImageJ (which at that time already existed, nicely), or could be converted and saved as extra file (!!) in 8bit mode, using the commercial TVIPS software EM-MENU3 (today, v.4). - And I think, the same holds true for quite a number of the early EM cameras. (maybe I am wrong?). Doing all this was part of my training of people using the EM. Necessarily.
} However, for stained material, there cannot be real intensity information needing } more than a few bits (or am I wrong about this?).
I would strongly argue that you are wrong about this ...
} When reducing bit depth, what is the best algorithm?
I would not trust any algorithm. This is part of the early steps in image processing, and people have to learn this as one of the first steps (in any lab, my argument). If you do not want to use commercial software, I would recommend ImageJ / Fiji - a software package which is "free" and public and excellent, since } } 20 yrs.
} Most reduction to 8 } bits that I've seen involve putting the bottom x% into 0, for instance } bottom 0.3% of pixel values } are assigned to black. Does this risk losing the ability to see fine } structure, or is this ok? Would it be preferable to not clip in the darks or is this ok?
No comment from my side.
} Is it ok to save only the 8 bit data and not bother with the 16 bit data?
simply, NO. Saving 8bit only is not ok, IMO.
} For instance, most people may not be prepared to deal with 16 } bit data or consider it inconvenient.
this is loss of scientific data ... just make people aware of this.
} Would very much like to know what is common practice and considered } acceptable for both optical and } electron microscopy images, both biological and material sciences.
my comments are common practice here. Although, I do not control everybody all the time. But if people ask me: see above.
to Chris "microwink" (whoever you are): basically, some of his arguments are similar. 1. Gatan hardware and software is widely distributed, and Gatan's sw can handle most of these tasks easily. There are image converters for Gatan's unique file format everywhere, and the rest, people have to learn. - I assume (although do not know) that other camera manufacturer offer similar software. At least TVIPS does. Since long. 2. 16bit vs 8bit data on Windows / Mac: this is not so much a problem of the operating system but of the 8bit displays we have. This is a longer story to explain. - But at the end, people have to learn to use proper SOFTWARE for analysing EM data and their histogram, and to save data in proper data format, for (a) scientific purposes , and for (b) generating slides for presentations and for publications. Again, there is a learning curve ... 3. it all depends what user want to do ... but people have to get aware, to gain responsibility (data compression!), and to get familiar with software. We are not taking pictures; we do generate scientific data. 4. sorry, Chris - NO: I do not tell people to store primary scientific data on Google server. NEVER. The are stored on University servers (tape roboter etc).
kind regards, Reinhard
-- Prof. Dr. Reinhard Rachel University of Regensburg Centre for EM / Anatomy Faculty of Biology & Preclin. Med. Universitaetsstrasse 31 D-93053 Regensburg - Germany tel +49 941 943 -2837, -1720 mail reinhard.rachel-at-biologie.uni-regensburg.de office: VKL 3.1.29 member of the IFSM board
Next microscopy conferences: - Microscopy Conference MC2019, 1.-5. Sept 2019 in Berlin - EMC2020 in Kopenhagen, 23.-28.8. 2020 (European conference) - MC2021 in Vienna (D-A-CH conference) - next Microbiol. conferences: VAAM 8.-11.03. 2020 Leipzig
==============================Original Headers============================== 20, 25 -- From Reinhard.Rachel-at-biologie.uni-regensburg.de Thu May 2 01:52:43 2019 20, 25 -- Received: from mx3.uni-regensburg.de (mx3.uni-regensburg.de [194.94.157.148]) 20, 25 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x426qgOM012087 20, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 2 May 2019 01:52:43 -0500 20, 25 -- Received: from mx3.uni-regensburg.de (localhost [127.0.0.1]) 20, 25 -- by localhost (Postfix) with SMTP id 95EF16000054 20, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 2 May 2019 08:50:33 +0200 (CEST) 20, 25 -- Received: from gwsmtp.uni-regensburg.de (gwsmtp1.uni-regensburg.de [132.199.5.51]) 20, 25 -- by mx3.uni-regensburg.de (Postfix) with ESMTP id 659056000050 20, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 2 May 2019 08:50:33 +0200 (CEST) 20, 25 -- Received: from uni-regensburg-smtp1-MTA by gwsmtp.uni-regensburg.de 20, 25 -- with Novell_GroupWise; Thu, 02 May 2019 08:50:33 +0200 20, 25 -- Message-Id: {5CCA93370200005400081216-at-gwsmtp.uni-regensburg.de} 20, 25 -- X-Mailer: Novell GroupWise Internet Agent 18.1.1 20, 25 -- Date: Thu, 02 May 2019 08:50:31 +0200 20, 25 -- From: "Reinhard Rachel" {Reinhard.Rachel-at-biologie.uni-regensburg.de} 20, 25 -- To: "microscopy listserver" {Microscopy-at-microscopy.com} 20, 25 -- Subject: 8 bit vs 16 bit images in all microscopy 20, 25 -- References: {201905020206.x4226264020002-at-microscopy.com} 20, 25 -- In-Reply-To: {201905020206.x4226264020002-at-microscopy.com} 20, 25 -- Mime-Version: 1.0 20, 25 -- Content-Type: text/plain; charset=US-ASCII 20, 25 -- Content-Disposition: inline 20, 25 -- Content-Transfer-Encoding: 8bit 20, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id x426qgOM012087 ==============================End of - Headers==============================
From the prospective of a materials science TEMmer, I believe it is never wise to discard the original, full bit-depth images acquired from the detector. For much of my work, the absolute value of the detected intensity (the number of high energy electrons per pixel) is important. The connection between the digital counts and the data we want is best known and most consistent for full bit depth images.
A fixed, linear mapping from 16 to 8 bits could be OK if the number of detected electrons is small. However, conversions to 8 bit designed for desktop publishing are dangerous because the look-up table used for conversion both changes for every image and (in general) is not preserved with the file. Once an unknown, undocumented transformation of the data has been made, the original intensity values are lost.
We routinely convert to 8 bit images to make figures or to share images with conveniently with colleagues, but we always keep the original data as close to the detector as we can. Disk space is cheap - keep your images!
Best wishes, Paul
Paul Voyles Professor, Department of Materials Science and Engineering Director, Wisconsin MRSEC Beckwith-Bascom Professor University of Wisconsin, Madison 1509 University Ave Madison, WI 53706 voice: 608-265-6740 / fax: 608-262-8353 paul.voyles-at-wisc.edu tem.msae.wisc.edu
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Email: michael.cammer-at-med.nyu.edu Name: Michael Cammer
Organization: NYU Langone
Title-Subject: [Filtered] 8 bit vs 16 bit images in all microscopy
Message: I am curious how people package digital data for themselves and other users. A few months ago on the confocal listserv we had a discussion regarding the sanctity of light microscopy data. One of the issues discussed was how to represent 12 to 16 bit data in an eight bit space.
Question: how do people routinely compress data into RGB for display and archiving and when is it permissible to not preserve the raw 12 to 16 bit raw data?
I have a similar question for TEM data. The new cameras on TEMs result in 16 bit images. However, for stained material, there cannot be real intensity information needing more than a few bits (or am I wrong about this?). When reducing bit depth, what is the best algorithm? Most reduction to 8 bits that I've seen involve putting the bottom x% into 0, for instance bottom 0.3% of pixel values are assigned to black. Does this risk losing the ability to see fine structure, or is this ok? Would it be preferable to not clip in the darks or is this ok? Is it ok to save only the 8 bit data and not bother with the 16 bit data? For instance, most people may not be prepared to deal with 16 bit data or consider it inconvenient.
Would very much like to know what is common practice and considered acceptable for both optical and electron microscopy images, both biological and material sciences.
Best regards- Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY 10016 Michael.Cammer-at-med.nyu.edu http://nyulmc.org/micros http://microscopynotes.com/ Voice direct only, no text or messages: 1-914-309-3270 and 1-646-501-0567
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I just wanted to add a comment to the reducing things to 8bit from the original image. An old mentor of mine would always run around reminding people that 1) data is data, you don't change it and 2) microscopy images are data. You wouldn't run an assay, then only save an interpretation of the data and not the original data. Should be the same here, you wouldn't take a image, then reduce bit depth requiring a loss of "scientific data" as Dr Reinhard mentioned, and only save that. You should always be able to get back to the original image as taken from the microscope, the only argument against this maybe is some much more data intensive techniques like light-sheet which even then there is some debate on what would be considered the original image that needs to be saved for archival reasons.
The only real problem with 12/16bit may be displaying it on Windows, which if you are using the photo viewer it shouldn't be for any real analysis. So for my users on a Leica confocal, I'll teach them how to manipulate the .lif file in ImageJ, and guide them towards using that for their analysis (at the very least how to open, mark, and save tiff files from) and then also save the final image from there, so they have the original image with all metadata and whatever bit depth it was taken at, and reduced/touched images for easy presentation.
And I would avoid any automated reduction if possible. Photoshop used to show you clipped pixels when changing curves, unsure if there is an easy option to do so in ImageJ but certainly possible. Then it becomes a question of whether what is being clipped either from the dark or bright regions is of value and how much is acceptable lost to the downstream analysis (if only presenting the image generally need to way over-saturate it, if doing mean intensity then your ROIs you want to avoid any over-saturation, etc).
} } } 02.05.2019 at 04:06: } I have a similar question for TEM data. The new cameras on TEMs result in 16 bit images.
this is not new, at all. 1st, after analog recording on negatives, and later digitization, I cannot remember to have stored 8bit data only, back in my early times in the 1980's. I have ALWAYS stored all data in 16bit mode. The first camera we bought in the 1990's (it was a used one), a TVIPS 1k x 1k camera, already recorded data with 12bit depth and stored them as 16bit files. These files could easily be handled in the 1990's by either ImageJ (which at that time already existed, nicely), or could be converted and saved as extra file (!!) in 8bit mode, using the commercial TVIPS software EM-MENU3 (today, v.4). - And I think, the same holds true for quite a number of the early EM cameras. (maybe I am wrong?). Doing all this was part of my training of people using the EM. Necessarily.
} However, for stained material, there cannot be real intensity information needing } more than a few bits (or am I wrong about this?).
I would strongly argue that you are wrong about this ...
} When reducing bit depth, what is the best algorithm?
I would not trust any algorithm. This is part of the early steps in image processing, and people have to learn this as one of the first steps (in any lab, my argument). If you do not want to use commercial software, I would recommend ImageJ / Fiji - a software package which is "free" and public and excellent, since } } 20 yrs.
} Most reduction to 8 } bits that I've seen involve putting the bottom x% into 0, for instance } bottom 0.3% of pixel values } are assigned to black. Does this risk losing the ability to see fine } structure, or is this ok? Would it be preferable to not clip in the darks or is this ok?
No comment from my side.
} Is it ok to save only the 8 bit data and not bother with the 16 bit data?
simply, NO. Saving 8bit only is not ok, IMO.
} For instance, most people may not be prepared to deal with 16 } bit data or consider it inconvenient.
this is loss of scientific data ... just make people aware of this.
} Would very much like to know what is common practice and considered } acceptable for both optical and } electron microscopy images, both biological and material sciences.
my comments are common practice here. Although, I do not control everybody all the time. But if people ask me: see above.
to Chris "microwink" (whoever you are): basically, some of his arguments are similar. 1. Gatan hardware and software is widely distributed, and Gatan's sw can handle most of these tasks easily. There are image converters for Gatan's unique file format everywhere, and the rest, people have to learn. - I assume (although do not know) that other camera manufacturer offer similar software. At least TVIPS does. Since long. 2. 16bit vs 8bit data on Windows / Mac: this is not so much a problem of the operating system but of the 8bit displays we have. This is a longer story to explain. - But at the end, people have to learn to use proper SOFTWARE for analysing EM data and their histogram, and to save data in proper data format, for (a) scientific purposes , and for (b) generating slides for presentations and for publications. Again, there is a learning curve ... 3. it all depends what user want to do ... but people have to get aware, to gain responsibility (data compression!), and to get familiar with software. We are not taking pictures; we do generate scientific data. 4. sorry, Chris - NO: I do not tell people to store primary scientific data on Google server. NEVER. The are stored on University servers (tape roboter etc).
kind regards, Reinhard
-- Prof. Dr. Reinhard Rachel University of Regensburg Centre for EM / Anatomy Faculty of Biology & Preclin. Med. Universitaetsstrasse 31 D-93053 Regensburg - Germany tel +49 941 943 -2837, -1720 mail reinhard.rachel-at-biologie.uni-regensburg.de office: VKL 3.1.29 member of the IFSM board
Next microscopy conferences: - Microscopy Conference MC2019, 1.-5. Sept 2019 in Berlin - EMC2020 in Kopenhagen, 23.-28.8. 2020 (European conference) - MC2021 in Vienna (D-A-CH conference) - next Microbiol. conferences: VAAM 8.-11.03. 2020 Leipzig
==============================Original Headers============================== 20, 25 -- From Reinhard.Rachel-at-biologie.uni-regensburg.de Thu May 2 01:52:43 2019 20, 25 -- Received: from mx3.uni-regensburg.de (mx3.uni-regensburg.de [194.94.157.148]) 20, 25 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x426qgOM012087 20, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 2 May 2019 01:52:43 -0500 20, 25 -- Received: from mx3.uni-regensburg.de (localhost [127.0.0.1]) 20, 25 -- by localhost (Postfix) with SMTP id 95EF16000054 20, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 2 May 2019 08:50:33 +0200 (CEST) 20, 25 -- Received: from gwsmtp.uni-regensburg.de (gwsmtp1.uni-regensburg.de [132.199.5.51]) 20, 25 -- by mx3.uni-regensburg.de (Postfix) with ESMTP id 659056000050 20, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 2 May 2019 08:50:33 +0200 (CEST) 20, 25 -- Received: from uni-regensburg-smtp1-MTA by gwsmtp.uni-regensburg.de 20, 25 -- with Novell_GroupWise; Thu, 02 May 2019 08:50:33 +0200 20, 25 -- Message-Id: {5CCA93370200005400081216-at-gwsmtp.uni-regensburg.de} 20, 25 -- X-Mailer: Novell GroupWise Internet Agent 18.1.1 20, 25 -- Date: Thu, 02 May 2019 08:50:31 +0200 20, 25 -- From: "Reinhard Rachel" {Reinhard.Rachel-at-biologie.uni-regensburg.de} 20, 25 -- To: "microscopy listserver" {Microscopy-at-microscopy.com} 20, 25 -- Subject: 8 bit vs 16 bit images in all microscopy 20, 25 -- References: {201905020206.x4226264020002-at-microscopy.com} 20, 25 -- In-Reply-To: {201905020206.x4226264020002-at-microscopy.com} 20, 25 -- Mime-Version: 1.0 20, 25 -- Content-Type: text/plain; charset=US-ASCII 20, 25 -- Content-Disposition: inline 20, 25 -- Content-Transfer-Encoding: 8bit 20, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id x426qgOM012087 ==============================End of - Headers==============================
==============================Original Headers============================== 31, 38 -- From rockman507-at-gmail.com Thu May 2 07:43:41 2019 31, 38 -- Received: from mail-pg1-f172.google.com (mail-pg1-f172.google.com [209.85.215.172]) 31, 38 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x42Chf88006600 31, 38 -- for {Microscopy-at-microscopy.com} ; Thu, 2 May 2019 07:43:41 -0500 31, 38 -- Received: by mail-pg1-f172.google.com with SMTP id p6so999973pgh.9 31, 38 -- for {Microscopy-at-microscopy.com} ; Thu, 02 May 2019 05:41:34 -0700 (PDT) 31, 38 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 31, 38 -- d=gmail.com; s=20161025; 31, 38 -- h=mime-version:from:date:message-id:subject:to; 31, 38 -- bh=7mZZ8K7ppHfTTDcGgSBYzmx4pnYIjzxg5J2zyuGuxRc=; 31, 38 -- b=ZiRWGbdb4ZHbIBVygF0hL0qKDPf7f5CjOsJHwTV1T4BfErOQENoFlonWBsR5M8uvns 31, 38 -- vl0z/M7EzbgGvswrLJQvoYhqx/42wEhxdXHg3O+h09ENRjiuXXuHJNKgcK+Gzvxxp3kA 31, 38 -- VEI5qUMgMhyaBB3p6GzvjWD+nNHH9e8ZNOnoEaKKQ3tSIGWw0iQIAUkJiK33gLlIBRWY 31, 38 -- r4nwXWXGjAW1eLPsOmAeputuMNl9UzZfsfysnhJiTyxtEKLLY9Gr0boMi5ZGIeGrNvGw 31, 38 -- 7CMPfzX4yVNcePEgZ6uJRMLNs5W4uPCrgJY/HLB0QC6co5XEZx2XsV1XrR+EY72NOeun 31, 38 -- q/Mw== 31, 38 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 31, 38 -- d=1e100.net; s=20161025; 31, 38 -- h=x-gm-message-state:mime-version:from:date:message-id:subject:to; 31, 38 -- bh=7mZZ8K7ppHfTTDcGgSBYzmx4pnYIjzxg5J2zyuGuxRc=; 31, 38 -- b=X1viQ5dG48M3rBwCl2we5R8cr7SRG2i6kqHuRgBiIVKTqeYMKvLOrjJSjhcx4D2HMt 31, 38 -- sC/d/w70gYpviA0sp1WUNreM1RekYd5lWsLI70SWmVmD98pzoDqBU3QjOdeK7sI6TEuN 31, 38 -- oa1GK7NqmaeNExGRG99EXcVrYmqEcVneUJgN06NJha2L4UJPU+r/Y9zqc1iJDFvXrhFh 31, 38 -- nIsLSPZNaRbbwok7NQk2tfejwVu9AlI36F+GnjgeYgKppt8uOOY+LdJVKvB40xiS7TWm 31, 38 -- B+4DrqFQUFiMTv0C34F44/JUUnm4EPuWqBPhSGFY3Q3/G27JtpM5QBvLHnyfDDTflLOS 31, 38 -- zW5w== 31, 38 -- X-Gm-Message-State: APjAAAXaYYnbZcNip887m7LwjyoLbR8dcqQ14NBEPrmsIqsm7rx5J9Ha 31, 38 -- 66SfDzq3ysRteYjv3Vy6KkO9Qv0QYfltEgX1Ypc3YvRz 31, 38 -- X-Google-Smtp-Source: APXvYqz2I+cij04exPPno2ZTN8P80arhyQhxxN9OVDK/GhoV9WhFu2qqbkYg2ywGMUc19Gr/nRz/lUD8gZ6QB95vUGo= 31, 38 -- X-Received: by 2002:aa7:980e:: with SMTP id e14mr3973028pfl.142.1556800893670; 31, 38 -- Thu, 02 May 2019 05:41:33 -0700 (PDT) 31, 38 -- MIME-Version: 1.0 31, 38 -- From: Jason Saredy {rockman507-at-gmail.com} 31, 38 -- Date: Thu, 2 May 2019 08:41:21 -0400 31, 38 -- Message-ID: {CA+cNCK24kG4UukS_36NdFEoVcUma2OW0Opy34A4J++qR6kUKHQ-at-mail.gmail.com} 31, 38 -- Subject: [Microscopy] 8 bit vs 16 bit images in all microscopy 31, 38 -- To: Microscopy-at-microscopy.com 31, 38 -- Content-Type: text/plain; charset="UTF-8" ==============================End of - Headers==============================
The MSA policy which has been in place since 2003 (and I helped establish this policy) is below. It clearly elucidates that all data needs to be save in the original “raw” data format. Compressing/altering in any form must be documented in all cases.
The argument that Windows and the users cannot display the data holds no water. Windows is not a scientist you and your users are, and you must control your data not a poorly written operating system, and the users also need to get properly trained to understand why.
The simple message is: if you recorded data at 8 bit’s that is fine, but don’t down sample (which changes the scientific content) and then call it real data.
I might add, just to finish the thought, don’t save data as JPEG or any “compressed” format. That also changes the scientific content. Use only fully non-compressed data formats (RAW, TIFF, PNG, HMSA….)
Nestor Your Friendly Neighborhood SysOp Who by the way currently is working at 32 & 64 bit’s as 16 is not nolonger sufficient for my science….
--------------------------------------------------------------------------------------------------------- "MSA Policy on Digital Imaging"
The MSA position on digital image processing has been approved as follows:
"Ethical digital imaging requires that the original uncompressed image file be stored on archival media (e.g., CD-R) without any image manipulation or processing operation. All parameters of the production and acquisition of this file, as well as any subsequent processing steps, must be documented and reported to ensure reproducibility.
Generally, acceptable (non-reportable) imaging operations include gamma correction, histogram stretching, and brightness and contrast adjustments. All other operations (such as Unsharp-Masking, Gaussian Blur, etc.) must be directly identified by the author as part of the experimental methodology. However, for diffraction data or any other image data that is used for subsequent quantification, all imaging operations must be reported."
This policy was formulated by the Digital Image Processing & Ethics Group of the MSA Education Committee and was adopted as MSA policy at the Summer Council meeting August 2-3, 2003." ————————————————————————————————————————————————————
=========================================== Dr. Nestor J. Zaluzec Argonne National Laboratory Photon Sciences Division 9700 S. Cass Ave Bldg 212 / A-143 Argonne, Illinois 60439 USA Email: Zaluzec-at-aaem.amc.anl.gov
Tel: 530-NES-TORZ (530-637-8679) has Voice Mail Lab: 630-252-7901
Senior Scientist - Argonne National Laboratory Fellow of the Microscopy Society of America Fellow of the MicroBeam Analysis Society Senior Institute Fellow - NAISE - Northwestern University Senior Institute Fellow - UChicago CASE E.P. Wigner Fellow - Oak Ridge National Laboratory Past President Microscopy Society of America Adjunct Professor of Physics - Northern Illinois University, University of Illinois at Chicago Visiting Professor of Microscopy - Manchester University , UK
Hi all, I generally agree with what's been said, but I think the "it's scientific data and therefore should never be deleted" argument has its limits. Anybody who uses an annular dark field or bright field detector in STEM is physically compressing 'scientific data'; as a lot of people have been showing lately, there is more information in the full spatially-resolved diffraction pattern for many samples. However, recording a spatially-resolved diffraction pattern at every probe position is obviously inconvenient if you don't have a fast camera, and since the compression that a STEM detector performs is generally well-characterized, we all accept the physical compression that happens when you only use a STEM detector and not a pixelated detector as a necessary loss of 'scientific data'.
As Chris pointed out, this argument applies to TEM images as well: if you just want to measure particle size, you can probably reduce bit depth and still produce a fully quantitative analysis. If there's a good reason to compress your image and you know exactly what you did, it's hard to argue that this is worse scientific practice than using a STEM detector.
Tyler --- Dr. Tyler Harvey Georg-August-Universität Göttingen IV. Physical Institute Friedrich-Hund-Platz 1 37077 Göttingen Germany
X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Thursday, May 2, 2019 2:56:06 PM To: Harvey, Tyler
This topic has a very long history. I will agree with Reinhard and others. Don’t go to 8bit’s. Upgrade your data visualization/analysis programs!
I also will refer everyone reading this post to the Microscopy Society of America Scientific Data Resource page which also addresses this issue:
Resources | Microscopy Society of America www.microscopy.org Microscopy Society of America (MSA)
The MSA policy which has been in place since 2003 (and I helped establish this policy) is below. It clearly elucidates that all data needs to be save in the original “raw” data format. Compressing/altering in any form must be documented in all cases.
The argument that Windows and the users cannot display the data holds no water. Windows is not a scientist you and your users are, and you must control your data not a poorly written operating system, and the users also need to get properly trained to understand why.
The simple message is: if you recorded data at 8 bit’s that is fine, but don’t down sample (which changes the scientific content) and then call it real data.
I might add, just to finish the thought, don’t save data as JPEG or any “compressed” format. That also changes the scientific content. Use only fully non-compressed data formats (RAW, TIFF, PNG, HMSA….)
Nestor Your Friendly Neighborhood SysOp Who by the way currently is working at 32 & 64 bit’s as 16 is not nolonger sufficient for my science….
--------------------------------------------------------------------------------------------------------- "MSA Policy on Digital Imaging"
The MSA position on digital image processing has been approved as follows:
"Ethical digital imaging requires that the original uncompressed image file be stored on archival media (e.g., CD-R) without any image manipulation or processing operation. All parameters of the production and acquisition of this file, as well as any subsequent processing steps, must be documented and reported to ensure reproducibility.
Generally, acceptable (non-reportable) imaging operations include gamma correction, histogram stretching, and brightness and contrast adjustments. All other operations (such as Unsharp-Masking, Gaussian Blur, etc.) must be directly identified by the author as part of the experimental methodology. However, for diffraction data or any other image data that is used for subsequent quantification, all imaging operations must be reported."
This policy was formulated by the Digital Image Processing & Ethics Group of the MSA Education Committee and was adopted as MSA policy at the Summer Council meeting August 2-3, 2003." ————————————————————————————————————————————————————
=========================================== Dr. Nestor J. Zaluzec Argonne National Laboratory Photon Sciences Division 9700 S. Cass Ave Bldg 212 / A-143 Argonne, Illinois 60439 USA Email: Zaluzec-at-aaem.amc.anl.gov
Tel: 530-NES-TORZ (530-637-8679) has Voice Mail Lab: 630-252-7901
Senior Scientist - Argonne National Laboratory Fellow of the Microscopy Society of America Fellow of the MicroBeam Analysis Society Senior Institute Fellow - NAISE - Northwestern University Senior Institute Fellow - UChicago CASE E.P. Wigner Fellow - Oak Ridge National Laboratory Past President Microscopy Society of America Adjunct Professor of Physics - Northern Illinois University, University of Illinois at Chicago Visiting Professor of Microscopy - Manchester University , UK
Excellent points. For universities using the Google Suite services integration, it's actually the university IT department who is in charge of data management, retention, and oversight. Of course, Google could peer into your data if they wanted, or were compelled to by a government agency, but there are easy ways to encrypt data uploaded to the cloud automatically using software like rsync or rclone. For sensitive data, like ITAR or corporate R&D, then obviously storing data on the cloud is prohibited. I'm not sure that images of gold nanoparticles, for example, warrant such careful scrutiny, but ultimately each user is responsible their own data.
Thanks, Chris
On Thu, May 2, 2019 at 2:51 AM {Reinhard.Rachel-at-biologie.uni-regensburg.de} wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Michael, } } } } } 02.05.2019 at 04:06: } } I have a similar question for TEM data. The new cameras on TEMs result in 16 bit images. } } this is not new, at all. } 1st, after analog recording on negatives, and later digitization, I cannot remember to have stored 8bit data only, back in my early times in the 1980's. I have ALWAYS stored all data in 16bit mode. } The first camera we bought in the 1990's (it was a used one), a TVIPS 1k x 1k camera, already recorded data with 12bit depth and stored them as 16bit files. These files could easily be handled in the 1990's by either ImageJ (which at that time already existed, nicely), or could be converted and saved as extra file (!!) in 8bit mode, using the commercial TVIPS software EM-MENU3 (today, v.4). - And I think, the same holds true for quite a number of the early EM cameras. (maybe I am wrong?). } Doing all this was part of my training of people using the EM. Necessarily. } } } However, for stained material, there cannot be real intensity information needing } } more than a few bits (or am I wrong about this?). } } I would strongly argue that you are wrong about this ... } } } When reducing bit depth, what is the best algorithm? } } I would not trust any algorithm. This is part of the early steps in image processing, and people have to learn this as one of the first steps (in any lab, my argument). If you do not want to use commercial software, I would recommend ImageJ / Fiji - a software package which is "free" and public and excellent, since } } 20 yrs. } } } Most reduction to 8 } } bits that I've seen involve putting the bottom x% into 0, for instance } } bottom 0.3% of pixel values } } are assigned to black. Does this risk losing the ability to see fine } } structure, or is this ok? Would it be preferable to not clip in the darks or is this ok? } } No comment from my side. } } } Is it ok to save only the 8 bit data and not bother with the 16 bit data? } } simply, NO. Saving 8bit only is not ok, IMO. } } } For instance, most people may not be prepared to deal with 16 } } bit data or consider it inconvenient. } } this is loss of scientific data ... just make people aware of this. } } } Would very much like to know what is common practice and considered } } acceptable for both optical and } } electron microscopy images, both biological and material sciences. } } my comments are common practice here. Although, I do not control everybody all the time. } But if people ask me: see above. } } to Chris "microwink" (whoever you are): } basically, some of his arguments are similar. } 1. Gatan hardware and software is widely distributed, and Gatan's sw can handle most of these tasks easily. There are image converters for Gatan's unique file format everywhere, and the rest, people have to learn. - I assume (although do not know) that other camera manufacturer offer similar software. At least TVIPS does. Since long. } 2. 16bit vs 8bit data on Windows / Mac: this is not so much a problem of the operating system but of the 8bit displays we have. This is a longer story to explain. - But at the end, people have to learn to use proper SOFTWARE for analysing EM data and their histogram, and to save data in proper data format, for (a) scientific purposes , and for (b) generating slides for presentations and for publications. Again, there is a learning curve ... } 3. it all depends what user want to do ... but people have to get aware, to gain responsibility (data compression!), and to get familiar with software. We are not taking pictures; we do generate scientific data. } 4. sorry, Chris - NO: I do not tell people to store primary scientific data on Google server. NEVER. The are stored on University servers (tape roboter etc). } } kind regards, } Reinhard } } -- } Prof. Dr. Reinhard Rachel } University of Regensburg } Centre for EM / Anatomy } Faculty of Biology & Preclin. Med. } Universitaetsstrasse 31 } D-93053 Regensburg - Germany } tel +49 941 943 -2837, -1720 } mail reinhard.rachel-at-biologie.uni-regensburg.de } office: VKL 3.1.29 } member of the IFSM board } } Next microscopy conferences: } - Microscopy Conference MC2019, 1.-5. Sept 2019 in Berlin } - EMC2020 in Kopenhagen, 23.-28.8. 2020 (European conference) } - MC2021 in Vienna (D-A-CH conference) } - next Microbiol. conferences: VAAM 8.-11.03. 2020 Leipzig } } } } ==============================Original Headers============================== } 20, 25 -- From Reinhard.Rachel-at-biologie.uni-regensburg.de Thu May 2 01:52:43 2019 } 20, 25 -- Received: from mx3.uni-regensburg.de (mx3.uni-regensburg.de [194.94.157.148]) } 20, 25 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x426qgOM012087 } 20, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 2 May 2019 01:52:43 -0500 } 20, 25 -- Received: from mx3.uni-regensburg.de (localhost [127.0.0.1]) } 20, 25 -- by localhost (Postfix) with SMTP id 95EF16000054 } 20, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 2 May 2019 08:50:33 +0200 (CEST) } 20, 25 -- Received: from gwsmtp.uni-regensburg.de (gwsmtp1.uni-regensburg.de [132.199.5.51]) } 20, 25 -- by mx3.uni-regensburg.de (Postfix) with ESMTP id 659056000050 } 20, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 2 May 2019 08:50:33 +0200 (CEST) } 20, 25 -- Received: from uni-regensburg-smtp1-MTA by gwsmtp.uni-regensburg.de } 20, 25 -- with Novell_GroupWise; Thu, 02 May 2019 08:50:33 +0200 } 20, 25 -- Message-Id: {5CCA93370200005400081216-at-gwsmtp.uni-regensburg.de} } 20, 25 -- X-Mailer: Novell GroupWise Internet Agent 18.1.1 } 20, 25 -- Date: Thu, 02 May 2019 08:50:31 +0200 } 20, 25 -- From: "Reinhard Rachel" {Reinhard.Rachel-at-biologie.uni-regensburg.de} } 20, 25 -- To: "microscopy listserver" {Microscopy-at-microscopy.com} } 20, 25 -- Subject: 8 bit vs 16 bit images in all microscopy } 20, 25 -- References: {201905020206.x4226264020002-at-microscopy.com} } 20, 25 -- In-Reply-To: {201905020206.x4226264020002-at-microscopy.com} } 20, 25 -- Mime-Version: 1.0 } 20, 25 -- Content-Type: text/plain; charset=US-ASCII } 20, 25 -- Content-Disposition: inline } 20, 25 -- Content-Transfer-Encoding: 8bit } 20, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id x426qgOM012087 } ==============================End of - Headers==============================
==============================Original Headers============================== 5, 45 -- From microwink-at-gmail.com Thu May 2 10:14:15 2019 5, 45 -- Received: from mail-it1-f178.google.com (mail-it1-f178.google.com [209.85.166.178]) 5, 45 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x42FEEeW024270 5, 45 -- for {Microscopy-at-microscopy.com} ; Thu, 2 May 2019 10:14:14 -0500 5, 45 -- Received: by mail-it1-f178.google.com with SMTP id k64so3976442itb.5 5, 45 -- for {Microscopy-at-microscopy.com} ; Thu, 02 May 2019 08:12:08 -0700 (PDT) 5, 45 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 5, 45 -- d=gmail.com; s=20161025; 5, 45 -- h=mime-version:references:in-reply-to:from:date:message-id:subject:to 5, 45 -- :cc:content-transfer-encoding; 5, 45 -- bh=pARuZv5wrCHDrCNqz4MtQ1ffQ6wbxdO5T6EP2s8TyzU=; 5, 45 -- b=GLuwup6EsSDI3h7bpB53iZsJQ2nvUOT8/EDoGPcBgxyC13mrhz/Ey3tfwl0Lhcf1uj 5, 45 -- HzbzPmMPSRtLejGv8Q7OkD3a0xLNsLqJH7xybUYfZYVDUV8ew4R/bZL9wiQ4QUPtr7Rk 5, 45 -- XyjRCwRXeiKKe5WkOTKS7MjNEPPcFcLovtEnIfU/9vW1mrlXe1GqhGvYsO07JE0dOTEB 5, 45 -- YbDVSs4rX8na5m53zhB49Fk+CBHbfBwjjjZDr3KFRtpktigDw6FJXBso094/boKEI+Ep 5, 45 -- eJuvHTlIrgQIf/Nsp75/G3pxtbDcSE100MLHDKZgUkYONeCIGkpAB6hh4a0o1S0BdScT 5, 45 -- OS+Q== 5, 45 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 5, 45 -- d=1e100.net; s=20161025; 5, 45 -- h=x-gm-message-state:mime-version:references:in-reply-to:from:date 5, 45 -- :message-id:subject:to:cc:content-transfer-encoding; 5, 45 -- bh=pARuZv5wrCHDrCNqz4MtQ1ffQ6wbxdO5T6EP2s8TyzU=; 5, 45 -- b=pbReJ7BSyU4OEFZyqOzQGV2mil8s/zdnxMagv455xBSbBLjgLOWvc3CyJ/LGz6D82x 5, 45 -- yN+QeidOwRCqnTsyEI2BfqofrLxgpjFnhMvT1SJFsIfBlw2Ha7ZJ0f4avyMdtSOmyXGa 5, 45 -- e6VDG/IfTTr1fW3qR51Gr5iBHsveS4pH6fMJqoXzUZVV5dnJvbeZ+o0cmv7xUGSe1YzS 5, 45 -- n43HZA8/I3/EbblEKWGwHFXEpNMvn4NRr5Ue8eRRkAvbbzrCbrpM+NVAsFxn82jtw50I 5, 45 -- 6kymDpoMMfBERsVvW9H5oZRfxakHWcYWRUlNzv2Rbz9lFDsKMW/N4X4wlODyOoACiNnP 5, 45 -- xdPg== 5, 45 -- X-Gm-Message-State: APjAAAU/6/SxHAKH8UW1WnIL8PgjXqlYsdHgBcdetr1Tgk977lxThhhQ 5, 45 -- ZJ2k/MNWmf85ORz+jIhzveWy+nPHHRUM1OPB4rI= 5, 45 -- X-Google-Smtp-Source: APXvYqyy6El4j2X3w6qqUTwhTQKSRmDsrzRHQe9tF29JJ+Qak7Fa8fkC9byIfdAk9QMRiHcNnsvWRn6IsicrFhpOGmo= 5, 45 -- X-Received: by 2002:a24:ad2:: with SMTP id 201mr2653558itw.26.1556809927814; 5, 45 -- Thu, 02 May 2019 08:12:07 -0700 (PDT) 5, 45 -- MIME-Version: 1.0 5, 45 -- References: {201905020653.x426reiS012844-at-microscopy.com} 5, 45 -- In-Reply-To: {201905020653.x426reiS012844-at-microscopy.com} 5, 45 -- From: Christopher Winkler {microwink-at-gmail.com} 5, 45 -- Date: Thu, 2 May 2019 11:11:55 -0400 5, 45 -- Message-ID: {CAA8T2PN7aCZS7oa5NH7w_fEta=qYEk+fJ4dSuo0nCkQaaEiSRw-at-mail.gmail.com} 5, 45 -- Subject: Re: [Microscopy] 8 bit vs 16 bit images in all microscopy 5, 45 -- To: Reinhard.Rachel-at-biologie.uni-regensburg.de 5, 45 -- Cc: Microscopy-at-microscopy.com 5, 45 -- Content-Type: text/plain; charset="UTF-8" 5, 45 -- Content-Transfer-Encoding: 8bit 5, 45 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id x42FEEeW024270 ==============================End of - Headers==============================
I agree with Reinhart Rachel and Paul Voyles. Always save the 16 bit image. Be careful and deliberate with image processing. The problem we face is that human eyes have a limited grayscale capacity and cannot distinguish all the gray levels in the 16 bit image. The microscopist's job is to faithfully represent the image information in the report.
There are colormaps that one can use to display an extended range of values. If you choose to do that, it is important to use a colorbar that shows the mappings. Some color mappings such as the 'jet' mapping that was the default in matplotlib was widely criticized for being misleading. The Viridis color map was developed to be a much better representation.
I am a firm believer that no image should be distributed without a description of how the image was recorded and processed. Reports with images with short captions and links to details are most helpful to your readers - and your self. The statistician Karl Broman wrote,
"Your closest collaborator is you six months from now and you do not respond to email."
I did a lot of microscopy, image processing, and image analysis during my career. Having such information in my archived reports helped when a client came to my lab months or even years later and wanted me to extend an analysis with new samples.
Best Regards, John Minter Retired from Kodak Analytical Sciences
==============================Original Headers============================== 6, 38 -- From jrminter-at-gmail.com Thu May 2 13:21:49 2019 6, 38 -- Received: from mail-lj1-f170.google.com (mail-lj1-f170.google.com [209.85.208.170]) 6, 38 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x42ILmaa009951 6, 38 -- for {Microscopy-at-microscopy.com} ; Thu, 2 May 2019 13:21:49 -0500 6, 38 -- Received: by mail-lj1-f170.google.com with SMTP id y8so2863413ljd.3 6, 38 -- for {Microscopy-at-microscopy.com} ; Thu, 02 May 2019 11:19:42 -0700 (PDT) 6, 38 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 38 -- d=gmail.com; s=20161025; 6, 38 -- h=mime-version:from:date:message-id:subject:to; 6, 38 -- bh=l2gk6TQSku3ayt2EpUTMMTooPY+0k606Lwalz7h/pKM=; 6, 38 -- b=I1xMLlQ4PHqQLgGCJx0VyvYixNWykO4JT+uYWEauk25237PiPA6rI2rJDoPF6r9GYm 6, 38 -- SFWCP2UvoTzkbU7OsSMCddQd6mTQdNhSj2sAp8Inb5ClIFaSNAatE7uc+JRNvcsT/U/v 6, 38 -- ZeTeoOf4puNsAeRB8z3GN6eBscVJzSDjt1WyalgP7n+sfzuBLnlS44wbgfnF8bjnZWJh 6, 38 -- HPHdPDNkjdFwiMgnXsFLjMW5ZL7XMFuiDKT3XMntk7HTj/2BcF8v4xLHUvtj8aCIVcg7 6, 38 -- L4MkFegz9uKtgreGGtMqHsnzgIgm41yJb3UGB1JPYyzzIU+PcTPBxfWpXWaiyrHluxk8 6, 38 -- dIQA== 6, 38 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 38 -- d=1e100.net; s=20161025; 6, 38 -- h=x-gm-message-state:mime-version:from:date:message-id:subject:to; 6, 38 -- bh=l2gk6TQSku3ayt2EpUTMMTooPY+0k606Lwalz7h/pKM=; 6, 38 -- b=ka+9Jy0i8D7r1q+CzlteOXkfYo2sxzF7Idveg6w58wnJa6NPISNOWAyBlS1RNoiR4s 6, 38 -- 75+uqzNkSTEs8X5+h5afM0X4l1OCEGJMNlQPpzpK0dF0p/b72Qj/95gsyT64LDRSl2tN 6, 38 -- QJDNcZHMK0eND7DZ9QqKOJFYP0y6ATbyeDPD7qEp6O1Wh/tgcj+5pQ9iVt5vdKwarwc/ 6, 38 -- tgvPGFQTvNk5vjK2X0FBLtR2Fq/Yh4DLQHLsfAgQA7IoubgjKNhFPHTQXPh3E5KJcUl2 6, 38 -- h1gLZQS/iZeJlZBVYdNesVXpqmpZ5pbnwZFi0N8hrRwzhXQoU0dMyEX9DfTM24qPI5u7 6, 38 -- cn1Q== 6, 38 -- X-Gm-Message-State: APjAAAVuG6AgciIXAriRx4sWdsYhu9dVMi+mj3fw0bX5JbxFQSk74EDB 6, 38 -- Tn0Kus2iQSBEROREIGqsbzFWfdikrB0HGlUYMyl226/2L20= 6, 38 -- X-Google-Smtp-Source: APXvYqyDV9S6NtwOr7cIkzILF3t2AWUXzpoQypJkDRFPgQuKwQkANXha/cPg0G88rdtXt1yHUd3NI/1Ld0zADBh+VEI= 6, 38 -- X-Received: by 2002:a2e:8496:: with SMTP id b22mr2718006ljh.9.1556821181977; 6, 38 -- Thu, 02 May 2019 11:19:41 -0700 (PDT) 6, 38 -- MIME-Version: 1.0 6, 38 -- From: John Minter {jrminter-at-gmail.com} 6, 38 -- Date: Thu, 2 May 2019 14:19:31 -0400 6, 38 -- Message-ID: {CABq4i1PdnL2e_a4b14et1gowdj0kqiM1H8i0OuoK7bvqxuhgdw-at-mail.gmail.com} 6, 38 -- Subject: Re: 8 bit vs 16 bit images in all microscopy 6, 38 -- To: Microscopy-at-microscopy.com 6, 38 -- Content-Type: text/plain; charset="UTF-8" ==============================End of - Headers==============================
The reason 8 bit images are so ubiquitous is that the human eye can only distinguish ~50 gray levels at one time. Rounding up to 64 levels gives 6 bits of dynamic range-then add one bit above and below to avoid clipping, and voila-8 bits! Any more for images displayed to humans is a waste. However, do what Nestor says: whatever raw data comes from the camera, save it. Storage costs today are infinitesimal.
A. John Mardinly, Ph.D., P.E. Classical Guitarist/Lutenist
} On May 2, 2019, at 11:22 AM, jrminter-at-gmail.com wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.com_MicroscopyListserver&d=DwIBAg&c=l45AxH-kUV29SRQusp9vYR0n1GycN4_2jInuKy6zbqQ&r=VLPJ8OE-c_C6joGeE1ftlvxMmQPq9N6mpKZONBRt90E&m=E02oWo101eaEDYuoARjzKnXMi88YVFcIUpoB3u6MLjs&s=YbvWymwOO_a0YrJgdT-U5uVHWLVT7CrzcFFw9_Xr0Ok&e= } On-Line Help https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.com_MicroscopyListserver_FAQ.html&d=DwIBAg&c=l45AxH-kUV29SRQusp9vYR0n1GycN4_2jInuKy6zbqQ&r=VLPJ8OE-c_C6joGeE1ftlvxMmQPq9N6mpKZONBRt90E&m=E02oWo101eaEDYuoARjzKnXMi88YVFcIUpoB3u6MLjs&s=05vTQIrptOLbBVfWAhtKum2x9zlTNWWmuLKCduBjVqA&e= } ---------------------------------------------------------------------------- } } I agree with Reinhart Rachel and Paul Voyles. Always save the 16 bit } image. Be careful and deliberate with image processing. The problem we } face is that human eyes have a limited grayscale capacity and cannot } distinguish all the gray levels in the 16 bit image. The } microscopist's job is to faithfully represent the image information in } the report. } } There are colormaps that one can use to display an extended range of } values. If you choose to do that, it is important to use a colorbar } that shows the mappings. Some color mappings such as the 'jet' mapping } that was the default in matplotlib was widely criticized for being } misleading. The Viridis color map was developed to be a much better } representation. } } I am a firm believer that no image should be distributed without a } description of how the image was recorded and processed. Reports with } images with short captions and links to details are most helpful to } your readers - and your self. The statistician Karl Broman wrote, } } "Your closest collaborator is you six months from now and you do } not respond to email." } } I did a lot of microscopy, image processing, and image analysis during } my career. Having such information in my archived reports helped when } a client came to my lab months or even years later and wanted me to } extend an analysis with new samples. } } Best Regards, } John Minter } Retired from Kodak Analytical Sciences } } ==============================Original Headers============================== } 6, 38 -- From jrminter-at-gmail.com Thu May 2 13:21:49 2019 } 6, 38 -- Received: from mail-lj1-f170.google.com (mail-lj1-f170.google.com [209.85.208.170]) } 6, 38 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x42ILmaa009951 } 6, 38 -- for {Microscopy-at-microscopy.com} ; Thu, 2 May 2019 13:21:49 -0500 } 6, 38 -- Received: by mail-lj1-f170.google.com with SMTP id y8so2863413ljd.3 } 6, 38 -- for {Microscopy-at-microscopy.com} ; Thu, 02 May 2019 11:19:42 -0700 (PDT) } 6, 38 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 6, 38 -- d=gmail.com; s=20161025; } 6, 38 -- h=mime-version:from:date:message-id:subject:to; } 6, 38 -- bh=l2gk6TQSku3ayt2EpUTMMTooPY+0k606Lwalz7h/pKM=; } 6, 38 -- b=I1xMLlQ4PHqQLgGCJx0VyvYixNWykO4JT+uYWEauk25237PiPA6rI2rJDoPF6r9GYm } 6, 38 -- SFWCP2UvoTzkbU7OsSMCddQd6mTQdNhSj2sAp8Inb5ClIFaSNAatE7uc+JRNvcsT/U/v } 6, 38 -- ZeTeoOf4puNsAeRB8z3GN6eBscVJzSDjt1WyalgP7n+sfzuBLnlS44wbgfnF8bjnZWJh } 6, 38 -- HPHdPDNkjdFwiMgnXsFLjMW5ZL7XMFuiDKT3XMntk7HTj/2BcF8v4xLHUvtj8aCIVcg7 } 6, 38 -- L4MkFegz9uKtgreGGtMqHsnzgIgm41yJb3UGB1JPYyzzIU+PcTPBxfWpXWaiyrHluxk8 } 6, 38 -- dIQA== } 6, 38 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 6, 38 -- d=1e100.net; s=20161025; } 6, 38 -- h=x-gm-message-state:mime-version:from:date:message-id:subject:to; } 6, 38 -- bh=l2gk6TQSku3ayt2EpUTMMTooPY+0k606Lwalz7h/pKM=; } 6, 38 -- b=ka+9Jy0i8D7r1q+CzlteOXkfYo2sxzF7Idveg6w58wnJa6NPISNOWAyBlS1RNoiR4s } 6, 38 -- 75+uqzNkSTEs8X5+h5afM0X4l1OCEGJMNlQPpzpK0dF0p/b72Qj/95gsyT64LDRSl2tN } 6, 38 -- QJDNcZHMK0eND7DZ9QqKOJFYP0y6ATbyeDPD7qEp6O1Wh/tgcj+5pQ9iVt5vdKwarwc/ } 6, 38 -- tgvPGFQTvNk5vjK2X0FBLtR2Fq/Yh4DLQHLsfAgQA7IoubgjKNhFPHTQXPh3E5KJcUl2 } 6, 38 -- h1gLZQS/iZeJlZBVYdNesVXpqmpZ5pbnwZFi0N8hrRwzhXQoU0dMyEX9DfTM24qPI5u7 } 6, 38 -- cn1Q== } 6, 38 -- X-Gm-Message-State: APjAAAVuG6AgciIXAriRx4sWdsYhu9dVMi+mj3fw0bX5JbxFQSk74EDB } 6, 38 -- Tn0Kus2iQSBEROREIGqsbzFWfdikrB0HGlUYMyl226/2L20= } 6, 38 -- X-Google-Smtp-Source: APXvYqyDV9S6NtwOr7cIkzILF3t2AWUXzpoQypJkDRFPgQuKwQkANXha/cPg0G88rdtXt1yHUd3NI/1Ld0zADBh+VEI= } 6, 38 -- X-Received: by 2002:a2e:8496:: with SMTP id b22mr2718006ljh.9.1556821181977; } 6, 38 -- Thu, 02 May 2019 11:19:41 -0700 (PDT) } 6, 38 -- MIME-Version: 1.0 } 6, 38 -- From: John Minter {jrminter-at-gmail.com} } 6, 38 -- Date: Thu, 2 May 2019 14:19:31 -0400 } 6, 38 -- Message-ID: {CABq4i1PdnL2e_a4b14et1gowdj0kqiM1H8i0OuoK7bvqxuhgdw-at-mail.gmail.com} } 6, 38 -- Subject: Re: 8 bit vs 16 bit images in all microscopy } 6, 38 -- To: Microscopy-at-microscopy.com } 6, 38 -- Content-Type: text/plain; charset="UTF-8" } ==============================End of - Headers==============================
==============================Original Headers============================== 8, 96 -- From John.Mardinly-at-asu.edu Thu May 2 14:41:38 2019 8, 96 -- Received: from bcnete02.asu.edu (bcnete02.asu.edu [52.15.170.116]) 8, 96 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x42JfXb4019302 8, 96 -- for {Microscopy-at-Microscopy.com} ; Thu, 2 May 2019 14:41:37 -0500 8, 96 -- X-ASG-Debug-ID: 1556825963-0df5cb346e1caacd0001-FOsErg 8, 96 -- Received: from mx0a-001d2c01.pphosted.com (ip-10-120-89-206.us-east-2.compute.internal [10.120.89.206]) by bcnete02.asu.edu with ESMTP id yseSJLrCG3wNQOWi (version=TLSv1.2 cipher=ECDHE-RSA-AES256-GCM-SHA384 bits=256 verify=NO); Thu, 02 May 2019 12:39:24 -0700 (PDT) 8, 96 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu 8, 96 -- X-Barracuda-RBL-Trusted-Forwarder: 10.120.89.206 8, 96 -- Received: from pps.filterd (m0116088.ppops.net [127.0.0.1]) 8, 96 -- by mx0a-001d2c01.pphosted.com (8.16.0.27/8.16.0.27) with SMTP id x42JXIjc008644; 8, 96 -- Thu, 2 May 2019 12:39:23 -0700 8, 96 -- Received: from nam05-co1-obe.outbound.protection.outlook.com (mail-co1nam05lp2054.outbound.protection.outlook.com [104.47.48.54]) 8, 96 -- by mx0a-001d2c01.pphosted.com with ESMTP id 2s85ge8bcs-1 8, 96 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES256-GCM-SHA384 bits=256 verify=NOT); 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If I could redirect the question a slightly different direction, how much of that data is useful and where does noise overwhelm the worthwhile data?
I admit that once the data has been collected, it should be saved. Even if there is a lot of noise, it may be possible to tease some worthwhile information out of it. Understand that I ask my question from the SEM side of the world and probably need a tutorial from someone on the TEM side.
Under normal circumstances on the SEM, even on good strong signals, noise dominates bits 7 and 8, and maybe even bit 6 or 5. I do a little exercise with new users to demonstrate this and try to persuade them that 16-bit images are a waste of space - at least in the SEM. I adjust contrast for the sample. I then try to find a nice homogeneous area, but to be sure, I take that area and raise the magnification to a hundred thousand times. The entire image should be at the same gray level. I collect an image under normal recording conditions (or at least set the dwell time to the same value of 30 us), and then look at the gray level histogram. If the peak is more than one gray level wide for an 8-bit image, and it always is, then the last bits are nothing but random noise. If the peak is 8 channels wide, then the last three bits are basically noise. And if bits 6-8 are noise, then why would I want to record bits 9-16 where they are nothing but noise?
Now this is where I need some instruction on the TEM side, on the SEM, we are adjusting the brightness and contrast to nicely fill the working range of the ADC. We get as much as we can out of those 8 bits. I would suppose that STEM in some imaging modes (e.g., HAADF) would be similar - that the output of the detector is scaled and then digitized. In other modes where a CCD is used to record the image, I suppose the count per pixel is collected as the raw value without scaling. What range of counts is usual? I certainly would expect they would routinely exceed 256 (8 bits). Maybe it is less than 4096 (12 bits) and certainly less than 65536 (16 bits). The count would determine the necessary depth for each pixel.
I thus infer that TEM software must scale the raw data before display. The bits would have been shifted by several positions to get a decent image. That might explain why a 16-bit TEM image fails to display properly in some applications. I suppose those apps simply cut off the lower 8 bits and display the upper 8 bits rather than scaling to the maximum value. If the most significant couple bits were zero, then the image would be confined to the lower quarter of the grayscale, which would not be very satisfactory.
If I have misunderstood, someone please instruct me - preferably gently.
Warren Straszheim, Ph.D. Iowa State University
-----Original Message----- X-from: rockman507-at-gmail.com [mailto:rockman507-at-gmail.com] Sent: Thursday, May 02, 2019 7:44 AM To: Straszheim, Warren E [BIOTC]
Hi all
I just wanted to add a comment to the reducing things to 8bit from the original image. An old mentor of mine would always run around reminding people that 1) data is data, you don't change it and 2) microscopy images are data. You wouldn't run an assay, then only save an interpretation of the data and not the original data. Should be the same here, you wouldn't take a image, then reduce bit depth requiring a loss of "scientific data" as Dr Reinhard mentioned, and only save that. You should always be able to get back to the original image as taken from the microscope, the only argument against this maybe is some much more data intensive techniques like light-sheet which even then there is some debate on what would be considered the original image that needs to be saved for archival reasons.
The only real problem with 12/16bit may be displaying it on Windows, which if you are using the photo viewer it shouldn't be for any real analysis. So for my users on a Leica confocal, I'll teach them how to manipulate the .lif file in ImageJ, and guide them towards using that for their analysis (at the very least how to open, mark, and save tiff files from) and then also save the final image from there, so they have the original image with all metadata and whatever bit depth it was taken at, and reduced/touched images for easy presentation.
And I would avoid any automated reduction if possible. Photoshop used to show you clipped pixels when changing curves, unsure if there is an easy option to do so in ImageJ but certainly possible. Then it becomes a question of whether what is being clipped either from the dark or bright regions is of value and how much is acceptable lost to the downstream analysis (if only presenting the image generally need to way over-saturate it, if doing mean intensity then your ROIs you want to avoid any over-saturation, etc).
} } } 02.05.2019 at 04:06: } I have a similar question for TEM data. The new cameras on TEMs result in 16 bit images.
this is not new, at all. 1st, after analog recording on negatives, and later digitization, I cannot remember to have stored 8bit data only, back in my early times in the 1980's. I have ALWAYS stored all data in 16bit mode. The first camera we bought in the 1990's (it was a used one), a TVIPS 1k x 1k camera, already recorded data with 12bit depth and stored them as 16bit files. These files could easily be handled in the 1990's by either ImageJ (which at that time already existed, nicely), or could be converted and saved as extra file (!!) in 8bit mode, using the commercial TVIPS software EM-MENU3 (today, v.4). - And I think, the same holds true for quite a number of the early EM cameras. (maybe I am wrong?). Doing all this was part of my training of people using the EM. Necessarily.
} However, for stained material, there cannot be real intensity } information needing more than a few bits (or am I wrong about this?).
I would strongly argue that you are wrong about this ...
} When reducing bit depth, what is the best algorithm?
I would not trust any algorithm. This is part of the early steps in image processing, and people have to learn this as one of the first steps (in any lab, my argument). If you do not want to use commercial software, I would recommend ImageJ / Fiji - a software package which is "free" and public and excellent, since } } 20 yrs.
} Most reduction to 8 } bits that I've seen involve putting the bottom x% into 0, for instance } bottom 0.3% of pixel values are assigned to black. Does this risk } losing the ability to see fine structure, or is this ok? Would it be } preferable to not clip in the darks or is this ok?
No comment from my side.
} Is it ok to save only the 8 bit data and not bother with the 16 bit data?
simply, NO. Saving 8bit only is not ok, IMO.
} For instance, most people may not be prepared to deal with 16 bit data } or consider it inconvenient.
this is loss of scientific data ... just make people aware of this.
} Would very much like to know what is common practice and considered } acceptable for both optical and electron microscopy images, both } biological and material sciences.
my comments are common practice here. Although, I do not control everybody all the time. But if people ask me: see above.
to Chris "microwink" (whoever you are): basically, some of his arguments are similar. 1. Gatan hardware and software is widely distributed, and Gatan's sw can handle most of these tasks easily. There are image converters for Gatan's unique file format everywhere, and the rest, people have to learn. - I assume (although do not know) that other camera manufacturer offer similar software. At least TVIPS does. Since long. 2. 16bit vs 8bit data on Windows / Mac: this is not so much a problem of the operating system but of the 8bit displays we have. This is a longer story to explain. - But at the end, people have to learn to use proper SOFTWARE for analysing EM data and their histogram, and to save data in proper data format, for (a) scientific purposes , and for (b) generating slides for presentations and for publications. Again, there is a learning curve ... 3. it all depends what user want to do ... but people have to get aware, to gain responsibility (data compression!), and to get familiar with software. We are not taking pictures; we do generate scientific data. 4. sorry, Chris - NO: I do not tell people to store primary scientific data on Google server. NEVER. The are stored on University servers (tape roboter etc).
kind regards, Reinhard
-- Prof. Dr. Reinhard Rachel University of Regensburg Centre for EM / Anatomy Faculty of Biology & Preclin. Med. Universitaetsstrasse 31 D-93053 Regensburg - Germany tel +49 941 943 -2837, -1720 mail reinhard.rachel-at-biologie.uni-regensburg.de office: VKL 3.1.29 member of the IFSM board
Next microscopy conferences: - Microscopy Conference MC2019, 1.-5. Sept 2019 in Berlin - EMC2020 in Kopenhagen, 23.-28.8. 2020 (European conference) - MC2021 in Vienna (D-A-CH conference) - next Microbiol. conferences: VAAM 8.-11.03. 2020 Leipzig
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You are warmly welcomed to submit the results of your research to the AVS66 meeting Nanometer Science and Technology Division (NSTD) sessions. NSTD explores the science and technology that emerges when material is shrunk to the nanoscale, including nanoscale devices and quantum systems exploiting nanoscale design and characterization, role of nanomaterials in novel devices and constructs, as well as surface chemistry, energetics, mechanics, and imagery.
This year, the specific emphasis will be made on the key connections between nanoscale physical and chemical phenomena induced in confined volumes as probed and manipulated by electron beams, scanning probe tips, electromagnetic radiation, ions, as well as approaches to harness these phenomena for nanoscale and atom-by-atom fabrication. The NSTD particularly promotes novel physical phenomena emerging in these nanosystems, and their applications for quantum information systems, sensing, and other applications.
The meeting features outstanding set of invited speakers in many areas of electron and scanning probe microscopy, including Ondrej Krivanek (NION) and Ilke Arslan (Argonne), Joe Stroscio (NIST), Paul Weiss (UCLA), and John Sader (U. Melbourne), and nanotechnology experts such as Chennupati Jagadish (ANU).
The detailed information about the program, graduate student and postdoctoral awards, and NSTD recognition award are available at
https://conta.cc/2TKJlnV
Looking forward to seeing you in Columbus, Ohio, on October 20-25!
Sergei
-- Sergei V. Kalinin
Director, Institute for Functional Imaging of Materials
Fellow MRS, IEEE, APS, IoP, AVS
Oak Ridge National Laboratory
Phone: (865) 241-0236
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I am looking for user / service manuals for the Jeol T 330 SEM.
Any help is appreciated very much.
Thanks,
Stefan
--
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
==============================Original Headers============================== 11, 23 -- From stefan.diller-at-t-online.de Fri May 3 14:21:34 2019 11, 23 -- Received: from mailout12.t-online.de (mailout12.t-online.de [194.25.134.22]) 11, 23 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x43JLXqg014874 11, 23 -- for {microscopy-at-microscopy.com} ; Fri, 3 May 2019 14:21:34 -0500 11, 23 -- Received: from fwd18.aul.t-online.de (fwd18.aul.t-online.de [172.20.26.244]) 11, 23 -- by mailout12.t-online.de (Postfix) with SMTP id 873F84187D97 11, 23 -- for {microscopy-at-microscopy.com} ; Fri, 3 May 2019 21:19:30 +0200 (CEST) 11, 23 -- Received: from mac-pro.local (JbCES-ZEghzd7gy15qKJ+kfViV7otvk-dG+J1SH66CepUlbriN1FB-f8Ri68RW8wIt-at-[84.167.156.31]) by fwd18.t-online.de 11, 23 -- with (TLSv1.2:ECDHE-RSA-AES256-GCM-SHA384 encrypted) 11, 23 -- esmtp id 1hMdiS-0WsibA0; Fri, 3 May 2019 21:19:28 +0200 11, 23 -- To: "Microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 11, 23 -- From: Stefan Diller {stefan.diller-at-t-online.de} 11, 23 -- Subject: Jeol T330 SEM manuals 11, 23 -- Message-ID: {eb089d06-8a0c-7932-32b2-1a13be3f44ec-at-t-online.de} 11, 23 -- Date: Fri, 3 May 2019 21:19:28 +0200 11, 23 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:60.0) 11, 23 -- Gecko/20100101 Thunderbird/60.6.1 11, 23 -- MIME-Version: 1.0 11, 23 -- Content-Type: text/plain; charset=windows-1252; format=flowed 11, 23 -- Content-Transfer-Encoding: 7bit 11, 23 -- Content-Language: en-GB 11, 23 -- X-ID: JbCES-ZEghzd7gy15qKJ+kfViV7otvk-dG+J1SH66CepUlbriN1FB-f8Ri68RW8wIt 11, 23 -- X-TOI-MSGID: 7d71d181-cc96-4523-9183-bfac544e4274 ==============================End of - Headers==============================
From pedugjuf5wfity-at-gmail.com Fri May 3 19:27:29 2019 Return-Path: {pedugjuf5wfity-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.163.205] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x440RR3s003200 for {microscopylistserverarchive7-at-microscopy.com} ; Fri, 3 May 2019 19:27:28 -0500 Received: from [105.204.97.208] by mail.webhostings4u.com with SMTP; Sat, 04 May 2019 12:11:57 +1200 Received: from mts.locks.grgtween.net ([Sat, 04 May 2019 11:52:19 +1200]) by nntp.pinxodet.net with ASMTP; Sat, 04 May 2019 11:52:19 +1200 Received: from mtu67.syds.piswix.net [110.72.135.246] by mail.webhostings4u.com with SMTP; Sat, 04 May 2019 11:51:40 +1200 Received: from m1.gns.snv.thisdomainl.com ([Sat, 04 May 2019 11:36:07 +1200]) by mail.webhostings4u.com with ASMTP; Sat, 04 May 2019 11:36:07 +1200 Received: from rsmail.alkoholic.net ([Sat, 04 May 2019 11:27:22 +1200]) by smtp18.yenddx.com with QMQP; Sat, 04 May 2019 11:27:22 +1200 Message-ID: {543701d5026a$e84850e0$632d5b56-at-pedugjuf5wfity}
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Organization: Danaher LSDX Title-Subject: [Filtered] Career Opportunitiy
Message: Current Openings:
Nanotechnology Consultant - Boston http://bit.ly/NanoLMS Cellular Imaging Sales and Applications Account Executive http://bit.ly/SSMOLDEV Boston, Chicago, San Diego, Houston
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Email: andrei.kolmakov-at-nist.gov Name: Andrei Kolmakov
Message: Dear Colleagues, The deadline for abstract submission for AVS 66 in Columbus , OH (Oct. 20-25, 2019)has been extended to May 8. We are looking forward to host you in our Chemical Analysis and Imaging Interfaces (CA) focus topic symposium. Please see details below. With best wishes
Andrei Kolmakov Physical Measurements Laboratory, NIST 100 Bureau Drive Gaithersburg, MD 20899 MS 6204
+++++++++++++++++++++++++++++++++++++ Chemical Analysis and Imaging Interfaces (CA) Call for Abstracts
Chemical and physical processes occurring at surfaces and interfaces, including gas-liquid, solid-liquid, and gas-solid interface are important in many applications and do represent grand scientific and engineering challenges. This symposium aims to provide a platform to the latest developments of emerging techniques and scientific understanding using in situ/ex situ/non situ/operando imaging, spectroscopy and microscopy to investigate challenging surfaces and interfaces. Contributed abstracts covering applications in biology, catalysis, energy conversion and storage, environment, and material sciences are welcome. Sessions and Speakers Gas-Liquid Interfacial Analysis and Imaging • Hendrik Bluhm, Lawrence Berkeley National Lab, "Liquid/Vapor Interfaces Investigated with Photoelectron Spectroscopy" • Vicki Grassian, Univ. of California, San Diego, "Chemical Analysis and Imaging of Environmental Interfaces" Solid-Liquid Interfacial Analysis and Imaging • Huolin Xin, Univ. of California, Irvine, “Artificial Intelligence--An Autonomous TEM for In-situ Studies" Gas-Solid Interfacial Analysis and Imaging • Jeong Young Park, Korea Advanced Institute of Science and Technology (KAIST), Republic of Korea, "Chemical Reactions on Bimetal Surfaces with Operando Surface Techniques" • Miquel B. Salmeron, Lawrence Berkeley National Lab, "Solid-liquid Interfaces: A New Surface Science Frontier" Liquid-Liquid and Solid-Solid Interfacial Analysis • Utkur Mirsaidov, National Univ. of Singapore, "In Situ Electron Microscopy in Studying Material Interfaces" • Roger Rousseau, Pacific Northwest National Lab, "Theoretical Investigation of Reactivity at Complex Solid-Liquid Interfaces" Progress and Challenges in Industrial Applications • Paul Dietrich, SPECS Surface Nano Analysis GmbH, "Interfacial Studies using Ambient Pressure XPS" • John Notte, Zeiss, "High Resolution Imaging of Challenging Material Interfaces" Novel Development and Approaches of Interfacial Analysis • Xiaoqing Pan, Univ. of California, Irvine, "Structure and Dynamics of Catalysts Under Atmospheric Gas Pressure" • Feng Wang, Brookhaven National Lab, "In Operando Spectroscopy and Microscopy of the Electrode-Electrolyte Interface in Batteries" Chemical Analysis and Imaging Interfaces Poster Session
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EM Facility in Center for Cellular & Molecular Imaging at Yale Medical School is seeking to hire an experienced electron microscopist to join our diverse team that cover wide spectrum of modern electron microscopy for biological sciences. This is a full-time and budgetary position, the incumbent will report to the core facility director.
Job responsibilities: - Able to learn and operate Focus-Ion-Beam SEM (FIB-SEM) for 3D data acquisition and subsequent image processing - Develop projects of correlative light and electron microscopy (CLEM) - Assist investigators in viewing and imaging with transmission electron microscopes - Train students or postdocs on independent operation of electron microscopes and ancillary equipment - Support user cryo EM operation in the facility. - Assist the facility director with educational tasks (classes, workshops etc.)
Requirements: - A Master's degree in biological or physical sciences - At least 3 years of experience in biological electron microscopy - Ability to learn new skills and techniques - Ability to perform complex imaging and data processing procedures - High degree of reliability, organizational and interpersonal skills - Ability to work independently and be team-oriented - Special consideration will be given to candidates who have advanced EM expertise such as FIB-SEM and electron tomography for biological samples
Please contact me if you are interested. The formal application needs to be done at STARS - Yale University's Online Hiring and Recruitment System. https://sjobs.brassring.com/TGnewUI/Search/Home/Home?partnerid=25053&siteid=5248#home
Sincerely
Xinran Liu, M.D. & Ph.D. Director, Center for Cellular & Molecular Imaging Bio & Cryo Electron Microscopy Core Facilities Yale University School of Medicine Office Phone: 203.785.4050 Lab Phone: 203.785.5390 http://medicine.yale.edu/ccmi/em
==============================Original Headers============================== 10, 60 -- From xinran.liu-at-yale.edu Sat May 4 11:15:46 2019 10, 60 -- Received: from NAM02-CY1-obe.outbound.protection.outlook.com (mail-eopbgr760135.outbound.protection.outlook.com [40.107.76.135]) 10, 60 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x44GFjVD001640 10, 60 -- for {microscopy-at-microscopy.com} ; Sat, 4 May 2019 11:15:45 -0500 10, 60 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yale.edu; s=selector1; 10, 60 -- h=From:Date:Subject:Message-ID:Content-Type:MIME-Version:X-MS-Exchange-SenderADCheck; 10, 60 -- bh=0JNk9lewKbI8M3bw4R9DB1Hc+kCeV5hhoVzjYflmHI0=; 10, 60 -- b=eJMEpBxRaIbJA4Ob+Q0xDO82Vx3eWfU77vkuCbf1zR1zwROq8R7s6uC8mTrzL8ndBiIdyJk1KVDTSwebPJOGLi+0+lVSAlqVTx3cjJbDfgEsKQgfyDDzggziWAhUKjOXuwp5pDP5lmfma84b7zCzcOIWzxmn9POA73QhuRSDIkU= 10, 60 -- Received: from BL0PR08MB4642.namprd08.prod.outlook.com (52.132.0.211) by 10, 60 -- BL0PR08MB5300.namprd08.prod.outlook.com (52.132.14.205) with Microsoft SMTP 10, 60 -- Server (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_256_GCM_SHA384) id 10, 60 -- 15.20.1856.12; 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From normanatlas54magaa-at-gmail.com Mon May 6 18:27:36 2019 Return-Path: {normanatlas54magaa-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.163.207] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x46NRVk4005894 for {microscopylistserverarchive7-at-microscopy.com} ; Mon, 6 May 2019 18:27:35 -0500 Received: from rsmail.alkoholic.net [170.71.99.89] by public.micromail.com.au with QMQP; Tue, 07 May 2019 07:09:46 +0800 Message-ID: {616E9FAC.33268A8B-at-gmail.com}
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Email: tim_thomas-at-tkd-inc.com Name: T Thomas
Title-Subject: [Filtered] Renishaw Raman Model 1000 Spectrometer
Message: Hello,
My older model 1000 Renishaw Raman unit is unable to connect to the computer. I am getting a "rack not found" error on the computer. I removed the back of the Renishaw, and the DC power supply is making a "click....click....click" sound, and the green LED's on the ends of the circuit boards flash at the same time as this sound. Does anyone have any suggestions for how to troubleshoot this problem?
Thank you very much! -Tim
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Shocked and dismayed to see that apparently the Zeiss interactive dye and filter database in no longer available, replaced by only static lists. Does anybody know if it's gone for good? It was way too handy...
Jim
--
James M. Ehrman Digital Microscopy Facility Mount Allison University 63B York St. Sackville, NB E4L 1G7 CANADA
Perhaps because I just went through a power supply failure on our evaporator, I am going to suggest checking out the power supply. Ours was rated for 24V and was only putting out 15V without a load. We were able to temporarily replace it with a beefy benchtop power supply. (The original power supply was rated for 7A.) It worked under the temporary supply, so we went ahead and purchased a replacement supply which happened to be considerably smaller and cheaper. So far, it is working fine.
Cautions: There is the question of what failed first. If something else failed and took out the power supply, it may just take out the next one as well.
You need to have some basic knowledge of electricity and how to deal with it safely. If you are in over your head, get help.
Warren Straszheim
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Tuesday, May 07, 2019 1:23 AM To: Straszheim, Warren E [BIOTC]
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Email: tim_thomas-at-tkd-inc.com Name: T Thomas
Title-Subject: [Filtered] Renishaw Raman Model 1000 Spectrometer
Message: Hello,
My older model 1000 Renishaw Raman unit is unable to connect to the computer. I am getting a "rack not found" error on the computer. I removed the back of the Renishaw, and the DC power supply is making a "click....click....click" sound, and the green LED's on the ends of the circuit boards flash at the same time as this sound. Does anyone have any suggestions for how to troubleshoot this problem?
Thank you very much! -Tim
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It is my great pleasure to announce that the University of Maryland Baltimore (UMB) Electron Microscopy Core Imaging Facility (EMCIF) will be hosting its fifth Annual Current EM Techniques Workshop on May 30th and 31st 2019. This year’s theme is “Multi-modality Imaging”. We have lined up speakers from Johns Hopkins University, George Washington University, New York University and UMB, as well as application specialists from Thermo Fisher, Tesacn, Angstrom Science and Nikon who will discuss research and imaging methods for the same specimen using multiple imaging platforms. Unique aspects of this workshop are the live instrument demonstrations and tryouts. This year, the instruments available for demonstration and tryout include an ASP1000 auto specimen processor by Microscopy Innovations, an ARTOS -3D Array Tomography Solutions from Leica Microsystems, a Hitachi TP4000 Table top SEM provided by Angstrom Scientific, an Access ultrathin Atomic Force Microscope by Angstrom Science, a Portable Plasma Cleaner/Asher from Ibss Group Inc, small laboratory automation instruments by EMS, and Glow discharge, Hi Vac coater by Ted Pella.
Thanks to generous support from our sponsors, we are again offering free participation in the workshop to students. Instrument demonstrations are also open. For more information, please visit the workshop website:
Finally, the workshop attendees will have an opportunity to join the local Chesapeake Microscopy & Microanalysis Society (CMMS) dinner also held at UMB on the evening of May 30th. Speakers for the CMMS dinner include Dr. Rhonda Stroud from the Naval Research Laboratory, also President of MAS, and Dr. Jiwen Zheng from FDA. Information about the CMMS dinner can be found at
https://www.dental.umaryland.edu/umb-cmms-dinner/
Please feel free to contact me if you have any questions regarding the workshop and dinner. I look forward to seeing you soon.
Best regards, Ru-ching rhsia-at-umaryland.edu Director, Electron Microscopy Core Imaging Facility University of Maryland Baltimore
Ru-ching Hsia
==============================Original Headers============================== 11, 57 -- From hsia627-at-hotmail.com Tue May 7 12:49:05 2019 11, 57 -- Received: from NAM04-CO1-obe.outbound.protection.outlook.com (mail-oln040092010073.outbound.protection.outlook.com [40.92.10.73]) 11, 57 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x47Hn5AK009649 11, 57 -- for {microscopy-at-microscopy.com} ; Tue, 7 May 2019 12:49:05 -0500 11, 57 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=hotmail.com; 11, 57 -- s=selector1; 11, 57 -- h=From:Date:Subject:Message-ID:Content-Type:MIME-Version:X-MS-Exchange-SenderADCheck; 11, 57 -- bh=ixLj73OC2lIBzXLm7/JDQWA5bO2icw3rfcsxwDc27jQ=; 11, 57 -- b=tXSwpxqVsLAwGDHVk0LC3USa7XO/tgJtj2hsD7iv9081TB1pns1A7CzHnPjQe9BWuWTmIie4rHFdt9THw4mtIs0NbQCSOZ+f9+YcgSSSnOGza1xL4x/pg/svw6Y5DzWDelGL9AwnGdWMK09ruI6HFwfYVUjL78mYX/oRVtyVnYT+ksLhd9Bdzl0qY7AHmg+HvsbYiYabCYO3oAkknbYMfjyagqahHrToVaFTxzwyzd7oPBr9nUKSzOvJ+kwnsJeHXTVO2+jLFJ9F0LnAIX+BCjPKLs1gBjWtM42yMM+WzoHSIp13a5tFwyGZLSn3OAO79Jlgc5YyK3wz1aNaetHrsw== 11, 57 -- Received: from BN3NAM04FT048.eop-NAM04.prod.protection.outlook.com 11, 57 -- (10.152.92.53) by BN3NAM04HT043.eop-NAM04.prod.protection.outlook.com 11, 57 -- (10.152.92.192) with Microsoft SMTP Server (version=TLS1_2, 11, 57 -- cipher=TLS_ECDHE_RSA_WITH_AES_256_CBC_SHA384) id 15.20.1835.13; Tue, 7 May 11, 57 -- 2019 17:47:14 +0000 11, 57 -- Received: from DM6PR04MB6073.namprd04.prod.outlook.com (10.152.92.55) by 11, 57 -- BN3NAM04FT048.mail.protection.outlook.com (10.152.92.210) with Microsoft SMTP 11, 57 -- Server (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_256_CBC_SHA384) id 11, 57 -- 15.20.1835.13 via Frontend Transport; Tue, 7 May 2019 17:47:14 +0000 11, 57 -- Received: from DM6PR04MB6073.namprd04.prod.outlook.com 11, 57 -- ([fe80::6460:b5a8:f05a:abd7]) by DM6PR04MB6073.namprd04.prod.outlook.com 11, 57 -- ([fe80::6460:b5a8:f05a:abd7%4]) with mapi id 15.20.1856.012; Tue, 7 May 2019 11, 57 -- 17:47:14 +0000 11, 57 -- From: Ruching hsia {hsia627-at-hotmail.com} 11, 57 -- To: "Microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 11, 57 -- CC: Ru-ching Hsia {rhsia-at-umaryland.edu} 11, 57 -- Subject: UMB Current EM Techniques Workshop, May 30-31, 2019 11, 57 -- Thread-Topic: UMB Current EM Techniques Workshop, May 30-31, 2019 11, 57 -- Thread-Index: AQHVBPxLuIhoG2GDy0O0KnASk81BXQ== 11, 57 -- Date: Tue, 7 May 2019 17:47:14 +0000 11, 57 -- Message-ID: {DM6PR04MB60739B7D2E1B21FC6DEB9D7CEF310-at-DM6PR04MB6073.namprd04.prod.outlook.com} 11, 57 -- Accept-Language: en-US 11, 57 -- Content-Language: en-US 11, 57 -- X-MS-Has-Attach: 11, 57 -- X-MS-TNEF-Correlator: 11, 57 -- x-incomingtopheadermarker: OriginalChecksum:CC3FEF18686157D82E489C059A9F6A0007E87CE65DEFC956E54979EF79BEA695;UpperCasedChecksum:E120BD4C5F7E8D932B2F427657F1CD4324B7BB213B586025198E598AFCF7E866;SizeAsReceived:6619;Count:42 11, 57 -- x-ms-exchange-messagesentrepresentingtype: 1 11, 57 -- x-tmn: [v2AdR/KfvmTUsq1Kj7z0N2tCKqx+r6SS] 11, 57 -- x-ms-publictraffictype: Email 11, 57 -- x-incomingheadercount: 42 11, 57 -- x-eopattributedmessage: 0 11, 57 -- x-microsoft-antispam: BCL:0;PCL:0;RULEID:(2390118)(5050001)(7020095)(20181119110)(201702061078)(5061506573)(5061507331)(1603103135)(2017031320274)(2017031324274)(2017031323274)(2017031322404)(1601125500)(1603101475)(1701031045);SRVR:BN3NAM04HT043; 11, 57 -- x-ms-traffictypediagnostic: BN3NAM04HT043: 11, 57 -- x-ms-exchange-purlcount: 2 11, 57 -- x-microsoft-antispam-message-info: 9ObLjM20SopEtEaog5shVZZ+xkHdhnhaoSU85VbFwbcX0OzPMkffkdrSmFufH3F7c9zNXXjYeWuAxzq/hWQ8qWf0uLj8ioQRwqxRgfCs4KyW8T8c9lPX7w0ise5Zkf8c8LYyBEFkmEyRLeHm2lRVGg82lgZ5amlUz5zLissa+UByNxodZoXn7jpew4jftEAw 11, 57 -- Content-Type: text/plain; charset="Windows-1252" 11, 57 -- MIME-Version: 1.0 11, 57 -- X-OriginatorOrg: hotmail.com 11, 57 -- X-MS-Exchange-CrossTenant-RMS-PersistedConsumerOrg: 00000000-0000-0000-0000-000000000000 11, 57 -- X-MS-Exchange-CrossTenant-Network-Message-Id: a152fbd0-0079-4b35-b48a-08d6d3140a1d 11, 57 -- X-MS-Exchange-CrossTenant-rms-persistedconsumerorg: 00000000-0000-0000-0000-000000000000 11, 57 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 07 May 2019 17:47:14.1509 11, 57 -- (UTC) 11, 57 -- X-MS-Exchange-CrossTenant-fromentityheader: Internet 11, 57 -- X-MS-Exchange-CrossTenant-id: 84df9e7f-e9f6-40af-b435-aaaaaaaaaaaa 11, 57 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: BN3NAM04HT043 11, 57 -- Content-Transfer-Encoding: 8bit 11, 57 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id x47Hn5AK009649 ==============================End of - Headers==============================
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Message: The Biology Department at the University of Puget Sound in Tacoma, WA has a Zeiss 902 TEM that is currently out of commission and since the faculty member that used to run it has retired, we are also looking to re-home it to anyone who might be able to make use of it. We had Dirk Dorneich work on it and I can provide his service report to anyone who might be interested. Thanks, Amy Replogle
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both mholman-at-mvainc.com, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Email: mholman-at-mvainc.com
Name: Melissa Holman
Organization: MVA Scientific Consultants
Title-Subject: [Filtered] Looking for old MAG*I*CAL (damaged is fine)
Message: We are on the hunt for an old MAG*I*CAL calibration standard someone is willing to part with. Damaged is fine – we just need a fragment, as long as some of the layers are still present. Please let me know if you have one you no longer need.
Thanks,
Melissa Holman Executive Director MVA Scientific Consultants 3300 Breckinridge Blvd Suite 400 Duluth, GA 30096 770.662.8509
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Join us for The Beckman Institute and Rigaku X-ray Microscopy Seminar and Workshop to learn about the latest applications of X-ray CT and microscopy in various research fields.
When: June 6th, Thursday 9:30 am - 5:00 pm
*** AM: Seminar by Invited Speakers *** "Next Generation Functional Lung Imaging" by Jonathan Dusting - 4Dx "X-ray Microscopy of Crystals in Amorphous Solid Dispersions" by Joseph P. Neilly - AbbVie, Inc. "Microstructural Analyses of Bone and Their Relation to Musculoskeletal Biomechanics" by Mariana Kersh - The Beckman Institute "High Resolution X-ray CT for Low Z Materials" by Aya Takase - Rigaku
*** PM: Workshop*** Lab workshop using X-ray microtomography and X-ray microscope systems
To lean more, visit: https://www.rigaku.com/mailers/2019/xrm/
Hope to see you there.
Aya Takase * Senior Scientist Rigaku Americas Corporation 9009 New Trails Drive * The Woodlands, TX 77381 USA T: 281-362-2300 ex 208 * F: 281-364-3628
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From duranelizabethe2rvbe-at-gmail.com Wed May 8 10:55:07 2019 Return-Path: {duranelizabethe2rvbe-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.163.206] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x48Fsrh4023443 for {microscopylistserverarchive7-at-microscopy.com} ; Wed, 8 May 2019 10:54:57 -0500 Received: from unknown (200.213.98.87) by mailout.endmonthnow.com with LOCAL; Wed, 08 May 2019 08:36:10 -0700 Received: from relay.2yahoo.com ([9.43.152.71]) by smtp.endend.nl with SMTP; Wed, 08 May 2019 08:23:49 -0700 Message-ID: {E5AF455D.F8A88732-at-gmail.com} charset="iso-8859-1" Content-Transfer-Encoding: quoted-printable
{HTML} {HEAD} {META name=3DGENERATOR content=3D"MSHTML 11.00.10570.1001"} {/HEAD} {body} I am Vice Chairman of Hang Seng Bank, I have Important Matter to Disc= uss with you concerning my late client. Died without a NEXT OF KIN. Send me= your private email for full details information. email me at {BR} E-Mail: {A= href=3D"mailto:draymdm-at-gmail.com"} draymdm-at-gmail.com {/A} {BR} Regards {BR} Mr.= Fung {/BODY} {/HTML}
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A very common failure mode in power supplies is the filtering electrolytic capacitors on the output side, they overheat and dries out, which leads to low capacitance and a more and more noisy power supply. A quick and easy test is to check (Power off and let it sit for a while to discharge! There's live terminals inside a power supply) if any capacitor is swollen up. Stroking the top of a capacitor with the finger tip will tell you immediately if it is like a dome or flat. If it is like a dome there is very high probability that replacing them will get the power supply back in order. If the top is flat it can still be broken, but now we're talking more traditional repair with measuring wave forms and voltages to find what is broken.
Göran
On 2019-05-07 08:23, microscopy.listserver-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy to both tim_thomas-at-tkd-inc.com, } Microscopy-at-Microscopy.com so that all Microscopy Listserver } Subscribers can benefit from our collective wisdom } --------------------------------------------------------------------------- } } Email: tim_thomas-at-tkd-inc.com Name: T Thomas } } Title-Subject: [Filtered] Renishaw Raman Model 1000 Spectrometer } } Message: Hello, } } My older model 1000 Renishaw Raman unit is unable to connect to the } computer. I am getting a "rack not found" error on the computer. I } removed the back of the Renishaw, and the DC power supply is making a } "click....click....click" sound, and the green LED's on the ends of the } circuit boards flash at the same time as this sound. Does anyone have } any suggestions for how to troubleshoot this problem? } } Thank you very much! } -Tim } } } Login Host: 73.96.100.81 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 10, 53 -- From microscopy.listserver-at-gmail.com Tue May 7 01:22:47 2019 } 10, 53 -- Received: from mail-wr1-f47.google.com (mail-wr1-f47.google.com [209.85.221.47]) } 10, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x476Mk0l021909 } 10, 53 -- for {microscopy-at-microscopy.com} ; Tue, 7 May 2019 01:22:46 -0500 } 10, 53 -- Received: by mail-wr1-f47.google.com with SMTP id v10so8212626wrt.6 } 10, 53 -- for {microscopy-at-microscopy.com} ; Mon, 06 May 2019 23:20:55 -0700 (PDT) } 10, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 10, 53 -- d=gmail.com; s=20161025; } 10, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 10, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 10, 53 -- bh=aCJfS8vWkoT8VpSVo0KTMio92lqJJqp4xkmWYkghG18=; } 10, 53 -- b=DMuA6WIdwSPTX4u2iyqqHY7sJPztuA4pUUV3ggy9bkrhMZFI8lXkgd5UhEii4FC8wE } 10, 53 -- xbY9W8A14bGaOCPwu776/85G6uEW5lM2CcqUBdJLW2yojN6oDOmDRlVcuDnkxQIZ0l4a } 10, 53 -- ex7L2kdWzjgKlPgdYwRr8xdHWO29uN3N/nR8TEMxYy+d8U0+x5RsLbtXoxpJr+rl91zG } 10, 53 -- uQB21NMSUlG6bDex6JFWElObjz7qsxHcpuEPTOTrC4Gun1QYpppdeVCq/P0LI1CPGrDD } 10, 53 -- jPHCfvQL8OM/SApkpMqFU34T2TBOur9MEzFfOn0+2BAMK7nEcp4zId+QFf4vi9BudCSc } 10, 53 -- 84Yw== } 10, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 10, 53 -- d=1e100.net; s=20161025; } 10, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 10, 53 -- :user-agent:mime-version:in-reply-to:content-language } 10, 53 -- :content-transfer-encoding; } 10, 53 -- bh=aCJfS8vWkoT8VpSVo0KTMio92lqJJqp4xkmWYkghG18=; } 10, 53 -- b=OcM4N1l6ZecGB1NFsDx6MY0igHTv6K6Bbtrk/b7lQ//mfLnYjSUFFlfiNjonR/Y5bX } 10, 53 -- ha/trxBdq4O7Vpw2Jiz1UfELaMtu+qaPOTKMry4fT7gOSOzICNXN5uub8vUdQ+lToNhb } 10, 53 -- n6FkQnFkfEs+3YruzXPJXeBxrojw6sbn0v0qcTRa9onJysCvzlW7bjpxbWxm/6LJ+pSZ } 10, 53 -- y3BnAUUVmfVZjLBu9rUjle8VzQ/AM99vEWax+ZLN+/Tn0OZiSo27d9liowYBeOae53z/ } 10, 53 -- /NGZl2byT2wPHN97c1O5kOov4rGFQQTSTcBq8plvKV7cabn7vdRpnfmpdgM4no2iQ4xy } 10, 53 -- u19A== } 10, 53 -- X-Gm-Message-State: APjAAAXuZOD1aHgvjrFhSDhTx7G/Cn/JDWXJTOcxTWjTu3mDjnY7lhRx } 10, 53 -- tKj7q2/Y4GGDbiP3H6V7R+Twnc+c } 10, 53 -- X-Google-Smtp-Source: APXvYqwU5jMeVqiCGQu1oGkbW29Tj/OoErOw46K7J0jSx47gfrPL5GmXRIrgtryUU0MmfAG9q6dpfQ== } 10, 53 -- X-Received: by 2002:adf:b641:: with SMTP id i1mr21011122wre.288.1557210054817; } 10, 53 -- Mon, 06 May 2019 23:20:54 -0700 (PDT) } 10, 53 -- Received: from Nestor-MacBookAir-Pro-2014-ElCapitan.local (66-203-142-46.pool.kielnet.net. [46.142.203.66]) } 10, 53 -- by smtp.googlemail.com with ESMTPSA id 91sm20666031wrs.43.2019.05.06.23.20.53 } 10, 53 -- for {microscopy-at-microscopy.com} } 10, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 10, 53 -- Mon, 06 May 2019 23:20:54 -0700 (PDT) } 10, 53 -- Subject: viaWWW:Renishaw Raman Model 1000 Spectrometer } 10, 53 -- References: {201905050033.x450XEKG003432-at-microscopy.com} } 10, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 10, 53 -- From: Microscopy Listserver {microscopy.listserver-at-gmail.com} } 10, 53 -- X-Forwarded-Message-Id: {201905050033.x450XEKG003432-at-microscopy.com} } 10, 53 -- Message-ID: {2e59e51e-026d-0b52-f178-c2760e8733e0-at-gmail.com} } 10, 53 -- Date: Tue, 7 May 2019 08:20:51 +0200 } 10, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.13; rv:60.0) } 10, 53 -- Gecko/20100101 Thunderbird/60.6.1 } 10, 53 -- MIME-Version: 1.0 } 10, 53 -- In-Reply-To: {201905050033.x450XEKG003432-at-microscopy.com} } 10, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 10, 53 -- Content-Language: en-US } 10, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
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Email: trevor-at-psu.edu
Name: Trevor Clark
Organization: Penn State
Title-Subject: [Filtered] FEI/Phillips Wehnelt
Message: Does anyone have an FEI/Philips Wehnelt for the W-filament Quanta or XL SEMs they are willing to sell? Trevor
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From klostocc091ufyae-at-gmail.com Thu May 9 18:09:56 2019 Return-Path: {klostocc091ufyae-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.163.199] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x49N9tjh020900 for {microscopylistserverarchive7-at-microscopy.com} ; Thu, 9 May 2019 18:09:56 -0500 Received: from smtp-server1.cfdenselr.com [130.228.75.132] by mx03.listsystemsf.net with ESMTP; Thu, 09 May 2019 18:50:46 -0400 Received: from [140.89.150.79] by mmx09.tilkbans.com with ASMTP; Thu, 09 May 2019 18:50:17 -0400 Received: from [41.15.2.191] by mail.webhostings4u.com with SMTP; Thu, 09 May 2019 18:45:39 -0400 Message-ID: {6550E228.A92184D4-at-gmail.com}
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Email: nizets2-at-yahoo.com Name: Stephane
Title-Subject: [Filtered] looking for a color camera for LM
Message: Dear colleagues,
I apologize in advance for mentionning LM. Our 14 years-old color axiocam died from exhaustion on our Axiovert 200M system (also 14-years old). The first, expensive solution would be to buy a new PC, new software upgrade (it was still axiovision at that time) and new camera to Zeiss but I don't need the listserv for this.
I was rather thinking about a much cheaper (although shorter term) alternative, where we would buy another color camera compatible with our actual system (Windows 2000 Professional!) and the Axiovision software. The connection was firewire. The camera is mainly used for fluorescence and normal histology so speed and high resolution are no priorities but rather sensitivity. Thank you in advance.
Best regards, Stephane
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==============================Original Headers============================== 11, 53 -- From microscopy.listserver-at-gmail.com Fri May 10 09:24:45 2019 11, 53 -- Received: from mail-wm1-f50.google.com (mail-wm1-f50.google.com [209.85.128.50]) 11, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x4AEOiMq000991 11, 53 -- for {microscopy-at-microscopy.com} ; Fri, 10 May 2019 09:24:45 -0500 11, 53 -- Received: by mail-wm1-f50.google.com with SMTP id j187so7744499wmj.1 11, 53 -- for {microscopy-at-microscopy.com} ; Fri, 10 May 2019 07:23:04 -0700 (PDT) 11, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 11, 53 -- d=gmail.com; s=20161025; 11, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version 11, 53 -- :in-reply-to:content-language:content-transfer-encoding; 11, 53 -- bh=lGPBuc+acJDwpvV/lv94UvvAym9BIvI3NA+UYs2dv6c=; 11, 53 -- b=PFEFxyZ6qarivaBTdE1/iYIo2VHPq+ou71czH0CNtBFK5lAVhzgg8EWKlzpPx+Z39S 11, 53 -- VwvqJkDsZczDQ0Gv5ADKWbchUozBrcfEca87idimktEN2IDLnic1Iv99kqfKeKN8DSqI 11, 53 -- cn2dXxXk3bHf5nBEKAqDlbavBjQY+O00hLkHmW8XArnNKG2htJgTCl8nHZQJGzzV4jcI 11, 53 -- 6M+zZbR3YKDoifvQpWntMyI4Y0gdsEyyKRKfVZw1+Txurdc/HLpGk4CRGfFwIl4ETtOd 11, 53 -- 8djKF8bbbvKEqnhhXPAZ1JSfQQE86XprkKaQKjcoSCuzKId23+wrTW542kF5s2nvthln 11, 53 -- i7pA== 11, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 11, 53 -- d=1e100.net; s=20161025; 11, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date 11, 53 -- :user-agent:mime-version:in-reply-to:content-language 11, 53 -- :content-transfer-encoding; 11, 53 -- bh=lGPBuc+acJDwpvV/lv94UvvAym9BIvI3NA+UYs2dv6c=; 11, 53 -- b=jubRtTCjk3g7HUh6hQKStoRg1PX/p/Ph+QGXQl7trpBTns8yqJ2EkX8P7dtw5DGP+e 11, 53 -- GDHdAv8sawV1wrBjeJSZbsQmsKm6nTYNSAt4AeT42Kh6dGB38FQKH0ASRqXQOFiKOXc5 11, 53 -- shhYIZ0k1biZ2P7NcZ2cBPoDREHX4fNvkwewxI4kJPMs/ZODSnQIX2lCfhMvREBiKpQa 11, 53 -- bNXxw5M+w77HkJuQuJVIhtis8E2LtwCIPDk8PGjCO4JwctFteudgGMbf9+6JDmnoBbBg 11, 53 -- 6ow8SDQEIGwuS1IqVsKD6VUbeRZk0n4IBx4iWsBTG8Wv2c1mBEA6jBsznEwytidPGioj 11, 53 -- eJAg== 11, 53 -- X-Gm-Message-State: APjAAAUqgfzIZHz4ej9geFtzn6Lc/AN+eZz3fcdKO5aKcj7mXF9uREM2 11, 53 -- EogfrgiN/QO6gtBz1dbueO9hX88uask= 11, 53 -- X-Google-Smtp-Source: APXvYqxcxb7s/CqKzGxp4B6ZFvoPng0vJkQ2p2TP+YaOftnI772ArnwlUn1+j4inCgStRfZDoMWIeQ== 11, 53 -- X-Received: by 2002:a1c:540e:: with SMTP id i14mr7124242wmb.57.1557498183697; 11, 53 -- Fri, 10 May 2019 07:23:03 -0700 (PDT) 11, 53 -- Received: from Nestor-MacBookAir-Pro-2014-ElCapitan.local ([77.60.103.234]) 11, 53 -- by smtp.googlemail.com with ESMTPSA id 4sm6313877wmi.4.2019.05.10.07.23.02 11, 53 -- for {microscopy-at-microscopy.com} 11, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 11, 53 -- Fri, 10 May 2019 07:23:03 -0700 (PDT) 11, 53 -- Subject: viaWWW:looking for a color camera for LM 11, 53 -- References: {201905100709.x4A799vK013891-at-microscopy.com} 11, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 11, 53 -- From: Microscopy Listserver {microscopy.listserver-at-gmail.com} 11, 53 -- X-Forwarded-Message-Id: {201905100709.x4A799vK013891-at-microscopy.com} 11, 53 -- Message-ID: {5b7aa254-5c21-cc69-c526-a180b7b9b8ce-at-gmail.com} 11, 53 -- Date: Fri, 10 May 2019 16:23:01 +0200 11, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.13; rv:60.0) 11, 53 -- Gecko/20100101 Thunderbird/60.6.1 11, 53 -- MIME-Version: 1.0 11, 53 -- In-Reply-To: {201905100709.x4A799vK013891-at-microscopy.com} 11, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed 11, 53 -- Content-Language: en-US 11, 53 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: KATE.PHELPS-at-UTSOUTHWESTERN.EDU Name: Kate Luby-Phelps
Organization: UT Southwestern Medical Center
Title-Subject: [Filtered] Part-time EM techs needed
Message: The UT Southwestern Electron Microscopy Facility is looking for qualified EM technicians in the DFW area to provide part-time help during times when our workload is especially high or we are shorthanded. The plan is to have one or two people, perhaps retirees, who would be willing to work on call for an hourly wage, kind of like substitute teachers. We envision that the work would be primarily sample processing and sectioning. Anyone interested please send your CV to me -at- kate.phelps-at-utsouthwestern.edu.
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When I looked at upgrading the AxioCam attached to an Axioscope I found a couple of interesting paths forward:
1) Zeiss offers demo/used cameras for competitive prices. They still stock CCD cameras that use firewire connections, so you might be able to find something that is a drop-in replacement, though I'm unsure if the quality will be better than your newly deceased camera as the newer generation CMOS cameras use USB3 connections and would require a PC upgrade. The PC upgrade process is pretty easy, however, and you don't necessarily need anything fancy.
2) You can add an adapter tube or two to enable the usage of a DSLR camera in lieu of the AxioCam. The DSLR adapter option is much cheaper than a camera from Zeiss, and the quality for BF imaging can be very good. Since you are fluorescence data, you'll want to choose your DSLR camera carefully. Unlike the microscope camera manufacturers, the DSLR makers usually do not publish spectral sensitivity data. You can find more information about how to adapt a DSLR to your microscope at this website: https://www.lmscope.com/en/Zeiss_axioskop_photography_en.html Be sure to check out the parts guide to match adapter tubes and DSLR mount adapters between camera and microscope!
Good luck, Chris
On Fri, May 10, 2019 at 10:24 AM {microscopy.listserver-at-gmail.com} wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy to both nizets2-at-yahoo.com, } Microscopy-at-Microscopy.com so that all Microscopy Listserver } Subscribers can benefit from our collective wisdom } --------------------------------------------------------------------------- } } Email: nizets2-at-yahoo.com Name: Stephane } } Title-Subject: [Filtered] looking for a color camera for LM } } Message: Dear colleagues, } } I apologize in advance for mentionning LM. } Our 14 years-old color axiocam died from exhaustion on our Axiovert 200M } system (also 14-years old). The first, expensive solution would be to } buy a new PC, new software upgrade (it was still axiovision at that } time) and new camera to Zeiss but I don't need the listserv for this. } } I was rather thinking about a much cheaper (although shorter term) } alternative, where we would buy another color camera compatible with our } actual system (Windows 2000 Professional!) and the Axiovision software. } The connection was firewire. 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-- Senior Research Associate Nanoscale Characterization and Fabrication Laboratory Institute for Critical Technology and Applied Science Virginia Tech (267) 496-0587
From devlinrobb1gritn-at-gmail.com Sun May 12 20:48:28 2019 Return-Path: {devlinrobb1gritn-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.163.208] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x4D1mQYo025012 for {microscopylistserverarchive7-at-microscopy.com} ; Sun, 12 May 2019 20:48:27 -0500 Received: from snmp.otwaloow.com [126.234.154.70] by smtp.doneohx.com with LOCAL; Sun, 12 May 2019 15:44:21 -1000 Received: from rsmail.alkoholic.net ([Sun, 12 May 2019 15:33:32 -1000]) by mts.locks.grgtween.net with ESMTP; Sun, 12 May 2019 15:33:32 -1000 Message-ID: {7FD59A27.50F041BB-at-gmail.com}
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Email: ychen103-at-uncc.edu
Name: Youxing
Organization: UNC CHARLOTTE
Title-Subject: [Filtered] about UranyLess (Lead Citrate) use to TEM
Message: Hello,
We have a TEM JEOL-2100 as an open facility. Recently, we have a new user, who needs to stain their bio samples by by UranyLess. I want to know whether their repetitive use of TEM cause residual to the holder and TEM?
Thanks,
Below is the feedback from university EHS after they visited the TEM lab:
"Lead Citrate is classified as an OSHA Reproductive Toxicant 1A that may damage the unborn child and is suspected to damage fertility. Because of this classification, a Standard Operating Procedure (SOP) is required for using the chemical. EHS will address SOP requirements with Dr. Chi.
For equipment contamination concerns, please contact Dr. Chi as EHS has no way of knowing if the samples containing lead will contaminate the TEM."
Login Host: 152.15.112.185 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
I thought, „UranyLess“ is a mixture of LANTHANides (I guess something like samarium and gadolinium &) but as I have seen, at least the "additional staining with Lead citrate - though another-higher concentration or company made - is recommended *) " I (and also others) should have a look into the catalogue of EMS (EMSdiasum…. In the USA) /Scientific Science Services (in Europe/Germany) to get proof about that (don’t know if they state the composition of the working solution in the dispenser one can order….)
"Lead citrate. Although UranyLess has a strong contasting effect, it is recommended to use lead citrate to enhance the contrast following the classic staining protocol according to Reynolds. We offer a ready to use 3% lead citrate solution in an Airless bottle which is filled w/o air and delivers the lead citrate droplets through a push-pump system thus eliminating CO2 contamination.
NB: no affiliation, no personal conflict or financial interest in the companies mentioned above….
As to my knowledge - as long as you don't overheat = glow or BURN your stained ultrahin sections - one should not expect at least HEAVY contamination within the column of TEM.... at least not more contamination than you can (would) expect if using the classic UO2Acetate staining sequence [which in the German version of "UranyLESS" (= no more radioactive URANYLacetate) description erroneously is termed: URAN-Acetate (instead of 'depleted' uranyl....... 'mimicking a huge health and threat risk as a radioactive hazard compound used in dirty bomb construction...) followed by Lead Citrate.
Other active and old fashioned TEM-Users have - just to tell - reported that the modern, state-of-the-art digital camera technique for TEM's is able to image your sections almost if not completely without classically staining your sections prior to viewing (but I always preferred classical /triple/ staining sequence when I was active in diagnostic TEM then....)
Best wishes and regards and good luck,
MUSS Wolfgang Dr. phil. (PhD) [OR i. R. / en retraite / retired]
FRMS, Retired Member of MSA Former Head of Electron Microscopy Lab at Institute of Pathology SALK-LKH / Salzburger Landeskliniken | General Hospital and PMU (private) PARACELSUS MEDICAL UNIVERSITY of SALZBURG Scientific Profile at ResearchGate: http://www.researchgate.net/profile/Wolfgang_MUSS inviting you to join RG (Sign up - ResearchGate -at- https://www.researchgate.net/signup.SignUp.html, and join 14+ million researchers, including 63 Nobel Laureates)
Former Secretary Long-standing Member (until March 2018) and (until June2017) Board Member of the
SCUR {The Society for Cutaneous Ultrastructure Research} „The Skin Imaging Society“ { www.scur.org }
============================================================================ ============ Von: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Gesendet: Donnerstag, 23. Mai 2019 13:31 An: wij.muss-at-aon.at Betreff: [Microscopy] about UranyLess (Lead Citrate) use to TEM
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please copy to both ychen103-at-uncc.edu, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom --------------------------------------------------------------------------- Email: ychen103-at-uncc.edu Name: Youxing Organization: UNC CHARLOTTE Title-Subject: about UranyLess (Lead Citrate) use to TEM Message:
Hello, We have a TEM JEOL-2100 as an open facility. Recently, we have a new user, who needs to stain their bio samples by by UranyLess. I want to know whether their repetitive use of TEM cause residual to the holder and TEM?
Thanks,
Below is the feedback from university EHS after they visited the TEM lab:
"Lead Citrate is classified as an OSHA Reproductive Toxicant 1A that may damage the unborn child and is suspected to damage fertility. Because of this classification, a Standard Operating Procedure (SOP) is required for using the chemical. EHS will address SOP requirements with Dr. Chi.
For equipment contamination concerns, please contact Dr. Chi as EHS has no way of knowing if the samples containing lead will contaminate the TEM."
Login Host: 152.15.112.185 Listserver Email Form V - 20120416 --------------------------------------------------------------------------- ==============================Original Headers============================== 15, 53 -- From microscopy.listserver-at-gmail.com Thu May 23 06:30:48 2019 15, 53 -- Received: from mail-it1-f169.google.com (mail-it1-f169.google.com [209.85.166.169]) 15, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x4NBUlOo021248 15, 53 -- for {microscopy-at-microscopy.com} ; Thu, 23 May 2019 06:30:48 -0500 15, 53 -- Received: by mail-it1-f169.google.com with SMTP id m141so9006420ita.3 15, 53 -- for {microscopy-at-microscopy.com} ; Thu, 23 May 2019 04:29:50 -0700 (PDT) 15, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 15, 53 -- d=gmail.com; s=20161025; 15, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version 15, 53 -- :in-reply-to:content-language:content-transfer-encoding; 15, 53 -- bh=jUmx1SLXR2tbai3I5FRAD55ZrjhSNe08tEEh+oife9k=; 15, 53 -- b=G62oWkZpSyuG/d0GRFtB4Rm5IAE09SlA0o5k/ZUU7KaWzvWVg/RF6x/xiz4HhbytOB 15, 53 -- jHsg/E4HCxvJt/MFlg2Pk18CKXjbXGamlwShpyP+TwS2UYpeYanodK0NXfUMxer7ha8u 15, 53 -- VQl2fgsZopnAJTPSEoeUUszyIUb24FskopMgdiPPFZfdEMUdN35SWg/ILHQCEQHccl/X 15, 53 -- lzCdvc9TW5ES0/9UA8+jCuVVJLjcwZu2pH+15bXi7weEa1TU2rR4/q8HUu59q9Jlgx3f 15, 53 -- CtvEH6Q0MLUtaTwMsOPT+nwvU/yq+HNkZUeCnnMeBX8G7N/Dfg2TdpFlFk6ujyFz9w0Z 15, 53 -- +wZQ== 15, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 15, 53 -- d=1e100.net; s=20161025; 15, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date 15, 53 -- :user-agent:mime-version:in-reply-to:content-language 15, 53 -- :content-transfer-encoding; 15, 53 -- bh=jUmx1SLXR2tbai3I5FRAD55ZrjhSNe08tEEh+oife9k=; 15, 53 -- b=pTGon8SXGtS6bMU8Liv8GDqLK7UbGyFbtbD2H8KDl6v5lsXcbke/slWR0qOElIHHhB 15, 53 -- 7qX3qLDXMn4GBIrMxlAFCr4RgBkM6e+bdTtO+QCwVLy+YaAG0ag9NAbjvmnefYXRTBvK 15, 53 -- jEZY9lgDzET6WOoL3/KX5TszpH87LlEjeeN07ZT3BUn9Dae+oHepSdA6HQemba7jeRWL 15, 53 -- DWrWsdZFWJHR+0pRPIHWiLWG3+egaCWuxbg2kfP4bD2hjfbo6p8MHa4lGYRyO6Ikp7F2 15, 53 -- LbirlRrcPXVn6oCL+Le91OqyjYYj6z4UQ5AkT03weCfK2BZWI8RlVBrakQ2zIr9tlLrM 15, 53 -- qD3w== 15, 53 -- X-Gm-Message-State: APjAAAUPFmXkMOQN7BdOigygLScbzkBe397qq1R0WfypvcsNjmhwSjCt 15, 53 -- TMWaPDzfGX0Xha2CMK9EWFt21chL 15, 53 -- X-Google-Smtp-Source: APXvYqwEDmTA4u1nGapPcDi5CX1qi46J9lHf/4c/phku40J50Y8HH6e9HIV/yJ/vYvm1KeJJQi6t xA== 15, 53 -- X-Received: by 2002:a24:b048:: with SMTP id b8mr12595196itj.115.1558610989391; 15, 53 -- Thu, 23 May 2019 04:29:49 -0700 (PDT) 15, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:1d02:c488:8254:e79d]) 15, 53 -- by smtp.googlemail.com with ESMTPSA id x99sm4212301ita.28.2019.05.23.04.29.48 15, 53 -- for {microscopy-at-microscopy.com} 15, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 15, 53 -- Thu, 23 May 2019 04:29:48 -0700 (PDT) 15, 53 -- Subject: viaWWW: about UranyLess (Lead Citrate) use to TEM 15, 53 -- References: {201905221740.x4MHe9FN014636-at-microscopy.com} 15, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 15, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} 15, 53 -- X-Forwarded-Message-Id: {201905221740.x4MHe9FN014636-at-microscopy.com} 15, 53 -- Message-ID: {0c5e76b3-ad5d-2e70-730f-73430f7e651a-at-gmail.com} 15, 53 -- Date: Thu, 23 May 2019 06:29:47 -0500 15, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.14; rv:60.0) 15, 53 -- Gecko/20100101 Thunderbird/60.7.0 15, 53 -- MIME-Version: 1.0 15, 53 -- In-Reply-To: {201905221740.x4MHe9FN014636-at-microscopy.com} 15, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed 15, 53 -- Content-Language: en-US 15, 53 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 36, 21 -- From wij.muss-at-aon.at Thu May 23 07:42:37 2019 36, 21 -- Received: from bsmtp7.bon.at (bsmtp7.bon.at [213.33.87.19]) 36, 21 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x4NCgbJ6031825 36, 21 -- for {Microscopy-at-Microscopy.com} ; Thu, 23 May 2019 07:42:37 -0500 36, 21 -- Received: from MussTHINK (unknown [93.83.25.98]) 36, 21 -- by bsmtp7.bon.at (Postfix) with ESMTPSA id 458pz66M0tz5tlG; 36, 21 -- Thu, 23 May 2019 14:41:38 +0200 (CEST) 36, 21 -- From: "MUSS Wolfgang Dr. phil./PhD \(OR i.R, retired\)" {wij.muss-at-aon.at} 36, 21 -- To: {Microscopy-at-Microscopy.com} 36, 21 -- Cc: {ychen103-at-uncc.edu} 36, 21 -- Subject: Re: [Microscopy] about UranyLess (Lead Citrate) use to TEM 36, 21 -- Date: Thu, 23 May 2019 14:41:40 +0200 36, 21 -- Message-ID: {000501d51164$dec1eaf0$9c45c0d0$-at-aon.at} 36, 21 -- MIME-Version: 1.0 36, 21 -- Content-Type: text/plain; 36, 21 -- charset="iso-8859-1" 36, 21 -- X-Mailer: Microsoft Outlook 15.0 36, 21 -- Thread-Index: AdURYc6UCcHMIXnoRQWBYgo+S1tiFQ== 36, 21 -- Content-Language: de 36, 21 -- Content-Transfer-Encoding: 8bit 36, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id x4NCgbJ6031825 ==============================End of - Headers==============================
I thought, „UranyLess“ is a mixture of LANTHANides (I guess something like samarium and gadolinium &) but as I have seen, at least the "additional staining with Lead citrate - though another-higher concentration or company made - is recommended *) " I (and also others) should have a look into the catalogue of EMS (EMSdiasum…. In the USA) /Scientific Science Services (in Europe/Germany) to get proof about that (don’t know if they state the composition of the working solution in the dispenser one can order….)
"Lead citrate. Although UranyLess has a strong contasting effect, it is recommended to use lead citrate to enhance the contrast following the classic staining protocol according to Reynolds. We offer a ready to use 3% lead citrate solution in an Airless bottle which is filled w/o air and delivers the lead citrate droplets through a push-pump system thus eliminating CO2 contamination. NB: no affiliation, no personal conflict or financial interest in the companies mentioned above….
As to my knowledge - as long as you don't overheat = glow or BURN your stained ultrahin sections - one should not expect at least HEAVY contamination within the column of TEM.... at least not more contamination than you can (would) expect if using the classic UO2Acetate staining sequence [which in the German version of "UranyLESS" (= no more radioactive URANYLacetate) description erroneously is termed: URAN-Acetate (instead of 'depleted' uranyl....... 'mimicking a huge health and threat risk as a radioactive hazard compound used in dirty bomb construction...) followed by Lead Citrate.
Other active and old fashioned TEM-Users have - just to tell - reported that the modern, state-of-the-art digital camera technique for TEM's is able to image your sections almost if not completely without classically staining your sections prior to viewing (but I always preferred classical /triple/ staining sequence when I was active in diagnostic TEM then....)
Best wishes and regards and good luck,
MUSS Wolfgang Dr. phil. (PhD) [OR i. R. / en retraite / retired] Ignaz-Rieder-Kai 19/6 A-5020 SALZBURG Österreich-AUSTRIA
Mobile-Tel.: 0043(0)676 5 369 456 E-mail: wij.muss-at-aon.at E-Mail altern.: womuss-at-gmail.com FRMS, Retired Member of MSA Former Head of Electron Microscopy Lab at Institute of Pathology SALK-LKH / Salzburger Landeskliniken | General Hospital and PMU (private) PARACELSUS MEDICAL UNIVERSITY of SALZBURG Scientific Profile at ResearchGate: http://www.researchgate.net/profile/Wolfgang_MUSS inviting you to join RG (Sign up - ResearchGate -at- https://www.researchgate.net/signup.SignUp.html, and join 14+ million researchers, including 63 Nobel Laureates)
Former Secretary Long-standing Member (until March 2018) and (until June2017) Board Member of the SCUR {The Society for Cutaneous Ultrastructure Research} „The Skin Imaging Society“ { www.scur.org }
============================================================================ ============ Von: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Gesendet: Donnerstag, 23. Mai 2019 13:31 An: wij.muss-at-aon.at Betreff: [Microscopy] about UranyLess (Lead Citrate) use to TEM
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
please copy to both ychen103-at-uncc.edu, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom --------------------------------------------------------------------------- Email: ychen103-at-uncc.edu Name: Youxing Organization: UNC CHARLOTTE Title-Subject: about UranyLess (Lead Citrate) use to TEM Message: Hello, We have a TEM JEOL-2100 as an open facility. Recently, we have a new user, who needs to stain their bio samples by by UranyLess. I want to know whether their repetitive use of TEM cause residual to the holder and TEM?
Thanks,
Below is the feedback from university EHS after they visited the TEM lab:
"Lead Citrate is classified as an OSHA Reproductive Toxicant 1A that may damage the unborn child and is suspected to damage fertility. Because of this classification, a Standard Operating Procedure (SOP) is required for using the chemical. EHS will address SOP requirements with Dr. Chi.
For equipment contamination concerns, please contact Dr. Chi as EHS has no way of knowing if the samples containing lead will contaminate the TEM."
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==============================Original Headers============================== 36, 21 -- From wij.muss-at-aon.at Thu May 23 07:42:37 2019 36, 21 -- Received: from bsmtp7.bon.at (bsmtp7.bon.at [213.33.87.19]) 36, 21 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x4NCgbJ6031825 36, 21 -- for {Microscopy-at-Microscopy.com} ; Thu, 23 May 2019 07:42:37 -0500 36, 21 -- Received: from MussTHINK (unknown [93.83.25.98]) 36, 21 -- by bsmtp7.bon.at (Postfix) with ESMTPSA id 458pz66M0tz5tlG; 36, 21 -- Thu, 23 May 2019 14:41:38 +0200 (CEST) 36, 21 -- From: "MUSS Wolfgang Dr. phil./PhD \(OR i.R, retired\)" {wij.muss-at-aon.at} 36, 21 -- To: {Microscopy-at-Microscopy.com} 36, 21 -- Cc: {ychen103-at-uncc.edu} 36, 21 -- Subject: Re: [Microscopy] about UranyLess (Lead Citrate) use to TEM 36, 21 -- Date: Thu, 23 May 2019 14:41:40 +0200 36, 21 -- Message-ID: {000501d51164$dec1eaf0$9c45c0d0$-at-aon.at} 36, 21 -- MIME-Version: 1.0 36, 21 -- Content-Type: text/plain; 36, 21 -- charset="iso-8859-1" 36, 21 -- X-Mailer: Microsoft Outlook 15.0 36, 21 -- Thread-Index: AdURYc6UCcHMIXnoRQWBYgo+S1tiFQ== 36, 21 -- Content-Language: de 36, 21 -- Content-Transfer-Encoding: 8bit 36, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id x4NCgbJ6031825 ==============================End of - Headers==============================
Stephane, I am impressed with color cameras from Amscope. Definitely inexpensive. My needs are not super rigorous. The have their own software (no idea if it goes back as far as Win2000). There are also Micro-manager driver(s) for Amscope cameras. I don't know about firewire but maybe your cpu box has a usb connection? You might have to upgrade the operating system/computer to get to Win 7 or like that. Not really sure. Hope these leads might help. Tobias
On 5/10/19 10:29 AM, microscopy.listserver-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe --http://www.microscopy.com/MicroscopyListserver } On-Line Helphttp://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form athttp://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy to bothnizets2-at-yahoo.com, } Microscopy-at-Microscopy.com so that all Microscopy Listserver } Subscribers can benefit from our collective wisdom } --------------------------------------------------------------------------- } } Email:nizets2-at-yahoo.com Name: Stephane } } Title-Subject: [Filtered] looking for a color camera for LM } } Message: Dear colleagues, } } I apologize in advance for mentionning LM. } Our 14 years-old color axiocam died from exhaustion on our Axiovert 200M } system (also 14-years old). The first, expensive solution would be to } buy a new PC, new software upgrade (it was still axiovision at that } time) and new camera to Zeiss but I don't need the listserv for this. } } I was rather thinking about a much cheaper (although shorter term) } alternative, where we would buy another color camera compatible with our } actual system (Windows 2000 Professional!) and the Axiovision software. } The connection was firewire. The camera is mainly used for fluorescence } and normal histology so speed and high resolution are no priorities but } rather sensitivity. } Thank you in advance. } } Best regards, } Stephane } } } Login Host: 213.33.126.84 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 11, 53 -- Frommicroscopy.listserver-at-gmail.com Fri May 10 09:24:45 2019 } 11, 53 -- Received: from mail-wm1-f50.google.com (mail-wm1-f50.google.com [209.85.128.50]) } 11, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x4AEOiMq000991 } 11, 53 -- for {microscopy-at-microscopy.com} ; Fri, 10 May 2019 09:24:45 -0500 } 11, 53 -- Received: by mail-wm1-f50.google.com with SMTP id j187so7744499wmj.1 } 11, 53 -- for {microscopy-at-microscopy.com} ; Fri, 10 May 2019 07:23:04 -0700 (PDT) } 11, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 11, 53 -- d=gmail.com; s=20161025; } 11, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 11, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 11, 53 -- bh=lGPBuc+acJDwpvV/lv94UvvAym9BIvI3NA+UYs2dv6c=; } 11, 53 -- b=PFEFxyZ6qarivaBTdE1/iYIo2VHPq+ou71czH0CNtBFK5lAVhzgg8EWKlzpPx+Z39S } 11, 53 -- VwvqJkDsZczDQ0Gv5ADKWbchUozBrcfEca87idimktEN2IDLnic1Iv99kqfKeKN8DSqI } 11, 53 -- cn2dXxXk3bHf5nBEKAqDlbavBjQY+O00hLkHmW8XArnNKG2htJgTCl8nHZQJGzzV4jcI } 11, 53 -- 6M+zZbR3YKDoifvQpWntMyI4Y0gdsEyyKRKfVZw1+Txurdc/HLpGk4CRGfFwIl4ETtOd } 11, 53 -- 8djKF8bbbvKEqnhhXPAZ1JSfQQE86XprkKaQKjcoSCuzKId23+wrTW542kF5s2nvthln } 11, 53 -- i7pA== } 11, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 11, 53 -- d=1e100.net; s=20161025; } 11, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 11, 53 -- :user-agent:mime-version:in-reply-to:content-language } 11, 53 -- :content-transfer-encoding; } 11, 53 -- bh=lGPBuc+acJDwpvV/lv94UvvAym9BIvI3NA+UYs2dv6c=; } 11, 53 -- b=jubRtTCjk3g7HUh6hQKStoRg1PX/p/Ph+QGXQl7trpBTns8yqJ2EkX8P7dtw5DGP+e } 11, 53 -- GDHdAv8sawV1wrBjeJSZbsQmsKm6nTYNSAt4AeT42Kh6dGB38FQKH0ASRqXQOFiKOXc5 } 11, 53 -- shhYIZ0k1biZ2P7NcZ2cBPoDREHX4fNvkwewxI4kJPMs/ZODSnQIX2lCfhMvREBiKpQa } 11, 53 -- bNXxw5M+w77HkJuQuJVIhtis8E2LtwCIPDk8PGjCO4JwctFteudgGMbf9+6JDmnoBbBg } 11, 53 -- 6ow8SDQEIGwuS1IqVsKD6VUbeRZk0n4IBx4iWsBTG8Wv2c1mBEA6jBsznEwytidPGioj } 11, 53 -- eJAg== } 11, 53 -- X-Gm-Message-State: APjAAAUqgfzIZHz4ej9geFtzn6Lc/AN+eZz3fcdKO5aKcj7mXF9uREM2 } 11, 53 -- EogfrgiN/QO6gtBz1dbueO9hX88uask= } 11, 53 -- X-Google-Smtp-Source: APXvYqxcxb7s/CqKzGxp4B6ZFvoPng0vJkQ2p2TP+YaOftnI772ArnwlUn1+j4inCgStRfZDoMWIeQ== } 11, 53 -- X-Received: by 2002:a1c:540e:: with SMTP id i14mr7124242wmb.57.1557498183697; } 11, 53 -- Fri, 10 May 2019 07:23:03 -0700 (PDT) } 11, 53 -- Received: from Nestor-MacBookAir-Pro-2014-ElCapitan.local ([77.60.103.234]) } 11, 53 -- by smtp.googlemail.com with ESMTPSA id 4sm6313877wmi.4.2019.05.10.07.23.02 } 11, 53 -- for {microscopy-at-microscopy.com} } 11, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 11, 53 -- Fri, 10 May 2019 07:23:03 -0700 (PDT) } 11, 53 -- Subject: viaWWW:looking for a color camera for LM } 11, 53 -- References: {201905100709.x4A799vK013891-at-microscopy.com} } 11, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 11, 53 -- From: Microscopy Listserver {microscopy.listserver-at-gmail.com} } 11, 53 -- X-Forwarded-Message-Id: {201905100709.x4A799vK013891-at-microscopy.com} } 11, 53 -- Message-ID: {5b7aa254-5c21-cc69-c526-a180b7b9b8ce-at-gmail.com} } 11, 53 -- Date: Fri, 10 May 2019 16:23:01 +0200 } 11, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.13; rv:60.0) } 11, 53 -- Gecko/20100101 Thunderbird/60.6.1 } 11, 53 -- MIME-Version: 1.0 } 11, 53 -- In-Reply-To: {201905100709.x4A799vK013891-at-microscopy.com} } 11, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 11, 53 -- Content-Language: en-US } 11, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
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From michhyma42it-at-gmail.com Fri May 24 15:32:07 2019 Return-Path: {michhyma42it-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.162.200] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x4OKW68H026683 for {microscopylistserverarchive7-at-microscopy.com} ; Fri, 24 May 2019 15:32:07 -0500 Received: from unknown (HELO rsmail.alkoholic.net) (Fri, 24 May 2019 09:25:40 -1100) by group21.345mail.com with SMTP; Fri, 24 May 2019 09:25:40 -1100 Received: from snmp.otwaloow.com ([Fri, 24 May 2019 09:12:17 -1100]) by smtp18.yenddx.com with SMTP; Fri, 24 May 2019 09:12:17 -1100 Received: from asx121.turbo-inline.com ([Fri, 24 May 2019 09:09:56 -1100]) by webmail.halftomorrow.com with LOCAL; Fri, 24 May 2019 09:09:56 -1100 Received: from unknown (199.85.22.103) by webmail.halftomorrow.com with SMTP; Fri, 24 May 2019 09:01:18 -1100 Message-ID: {66C0B44E.02D22D81-at-gmail.com}
I already contacted them but unfortunately I stopped the communication because of a lack of enthousiasm and will to help from their side.
They reply in one short sentence, it gives me the feeling that I disturb them and they answer just to be polite :-)
In the end, to be honest, the concurrents were much more interested in my request so I chose others. If you are interested to know, Thorlabs and photometrics gave me the best impression. Photometrics has a very competent and nice guy in Germany but they can only offer 1 color camera from QImaging.
Best regards, Stephane
On Thursday, May 23, 2019, 2:17:11 PM UTC, microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} wrote:
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-------- Forwarded Message -------- X-from: Â Â Â Tobias Baskin {baskin-at-bio.umass.edu {mailto:baskin-at-bio.umass.edu} }
Stephane, Â Â Â Â Â Â Â Â Â Â Â Â I am impressed with color cameras from Amscope. Definitely inexpensive. My needs are not super rigorous. The have their own software (no idea if it goes back as far as Win2000). There are also Micro-manager driver(s) for Amscope cameras. I don't know about firewire but maybe your cpu box has a usb connection? You might have to upgrade the operating system/computer to get to Win 7 or like that. Not really sure. Hope these leads might help. Tobias
On 5/10/19 10:29 AM, microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe --http://www.microscopy.com/MicroscopyListserver } On-Line Helphttp://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form athttp://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy to bothnizets2-at-yahoo.com {mailto:bothnizets2-at-yahoo.com} , } Microscopy-at-Microscopy.com {mailto:Microscopy-at-Microscopy.com}  so that all Microscopy Listserver } Subscribers can benefit from our collective wisdom } --------------------------------------------------------------------------- } } Email:nizets2-at-yahoo.com {mailto:nizets2-at-yahoo.com}  Name: Stephane } } Title-Subject: [Filtered] looking for a color camera for LM } } Message: Dear colleagues, } } I apologize in advance for mentionning LM. } Our 14 years-old color axiocam died from exhaustion on our Axiovert 200M } system (also 14-years old). The first, expensive solution would be to } buy a new PC, new software upgrade (it was still axiovision at that } time) and new camera to Zeiss but I don't need the listserv for this. } } I was rather thinking about a much cheaper (although shorter term) } alternative, where we would buy another color camera compatible with our } actual system (Windows 2000 Professional!) and the Axiovision software. } The connection was firewire. The camera is mainly used for fluorescence } and normal histology so speed and high resolution are no priorities but } rather sensitivity. } Thank you in advance. } } Best regards, } Stephane } } }   Login Host: 213.33.126.84 }   Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 11, 53 -- Frommicroscopy.listserver-at-gmail.com {mailto:Frommicroscopy.listserver-at-gmail.com}  Fri May 10 09:24:45 2019 } 11, 53 -- Received: from mail-wm1-f50.google.com (mail-wm1-f50.google.com [209.85.128.50]) } 11, 53 --    by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x4AEOiMq000991 } 11, 53 --    for {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } ; Fri, 10 May 2019 09:24:45 -0500 } 11, 53 -- Received: by mail-wm1-f50.google.com with SMTP id j187so7744499wmj.1 } 11, 53 --    for {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } ; Fri, 10 May 2019 07:23:04 -0700 (PDT) } 11, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 11, 53 --    d=gmail.com; s=20161025; } 11, 53 --    h=subject:references:to:from:message-id:date:user-agent:mime-version } 11, 53 --     :in-reply-to:content-language:content-transfer-encoding; } 11, 53 --    bh=lGPBuc+acJDwpvV/lv94UvvAym9BIvI3NA+UYs2dv6c=; } 11, 53 --    b=PFEFxyZ6qarivaBTdE1/iYIo2VHPq+ou71czH0CNtBFK5lAVhzgg8EWKlzpPx+Z39S } 11, 53 --     VwvqJkDsZczDQ0Gv5ADKWbchUozBrcfEca87idimktEN2IDLnic1Iv99kqfKeKN8DSqI } 11, 53 --     cn2dXxXk3bHf5nBEKAqDlbavBjQY+O00hLkHmW8XArnNKG2htJgTCl8nHZQJGzzV4jcI } 11, 53 --     6M+zZbR3YKDoifvQpWntMyI4Y0gdsEyyKRKfVZw1+Txurdc/HLpGk4CRGfFwIl4ETtOd } 11, 53 --     8djKF8bbbvKEqnhhXPAZ1JSfQQE86XprkKaQKjcoSCuzKId23+wrTW542kF5s2nvthln } 11, 53 --     i7pA== } 11, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 11, 53 --    d=1e100.net; s=20161025; } 11, 53 --    h=x-gm-message-state:subject:references:to:from:message-id:date } 11, 53 --     :user-agent:mime-version:in-reply-to:content-language } 11, 53 --     :content-transfer-encoding; } 11, 53 --    bh=lGPBuc+acJDwpvV/lv94UvvAym9BIvI3NA+UYs2dv6c=; } 11, 53 --    b=jubRtTCjk3g7HUh6hQKStoRg1PX/p/Ph+QGXQl7trpBTns8yqJ2EkX8P7dtw5DGP+e } 11, 53 --     GDHdAv8sawV1wrBjeJSZbsQmsKm6nTYNSAt4AeT42Kh6dGB38FQKH0ASRqXQOFiKOXc5 } 11, 53 --     shhYIZ0k1biZ2P7NcZ2cBPoDREHX4fNvkwewxI4kJPMs/ZODSnQIX2lCfhMvREBiKpQa } 11, 53 --     bNXxw5M+w77HkJuQuJVIhtis8E2LtwCIPDk8PGjCO4JwctFteudgGMbf9+6JDmnoBbBg } 11, 53 --     6ow8SDQEIGwuS1IqVsKD6VUbeRZk0n4IBx4iWsBTG8Wv2c1mBEA6jBsznEwytidPGioj } 11, 53 --     eJAg== } 11, 53 -- X-Gm-Message-State: APjAAAUqgfzIZHz4ej9geFtzn6Lc/AN+eZz3fcdKO5aKcj7mXF9uREM2 } 11, 53 --    EogfrgiN/QO6gtBz1dbueO9hX88uask= } 11, 53 -- X-Google-Smtp-Source: APXvYqxcxb7s/CqKzGxp4B6ZFvoPng0vJkQ2p2TP+YaOftnI772ArnwlUn1+j4inCgStRfZDoMWIeQ== } 11, 53 -- X-Received: by 2002:a1c:540e:: with SMTP id i14mr7124242wmb.57.1557498183697; } 11, 53 --    Fri, 10 May 2019 07:23:03 -0700 (PDT) } 11, 53 -- Received: from Nestor-MacBookAir-Pro-2014-ElCapitan.local ([77.60.103.234]) } 11, 53 --    by smtp.googlemail.com with ESMTPSA id 4sm6313877wmi.4.2019.05.10.07.23.02 } 11, 53 --    for {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } } 11, 53 --    (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 11, 53 --    Fri, 10 May 2019 07:23:03 -0700 (PDT) } 11, 53 -- Subject: viaWWW:looking for a color camera for LM } 11, 53 -- References: {201905100709.x4A799vK013891-at-microscopy.com {mailto:201905100709.x4A799vK013891-at-microscopy.com} } } 11, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } } 11, 53 -- From: Microscopy Listserver {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } } 11, 53 -- X-Forwarded-Message-Id: {201905100709.x4A799vK013891-at-microscopy.com {mailto:201905100709.x4A799vK013891-at-microscopy.com} } } 11, 53 -- Message-ID: {5b7aa254-5c21-cc69-c526-a180b7b9b8ce-at-gmail.com {mailto:5b7aa254-5c21-cc69-c526-a180b7b9b8ce-at-gmail.com} } } 11, 53 -- Date: Fri, 10 May 2019 16:23:01 +0200 } 11, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.13; rv:60.0) } 11, 53 -- Gecko/20100101 Thunderbird/60.6.1 } 11, 53 -- MIME-Version: 1.0 } 11, 53 -- In-Reply-To: {201905100709.x4A799vK013891-at-microscopy.com {mailto:201905100709.x4A799vK013891-at-microscopy.com} } } 11, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 11, 53 -- Content-Language: en-US } 11, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
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Email: shaojiew-at-nanolab1.com
Name: Jeff (Shaojie) Wang
Organization: Nanolab
Title-Subject: [Filtered] JEOL2500 TEM for donation to educational institution
Message: We have a JEOL2500 S/TEM here that we want to donate to school/university. The tool is in good condition and can get atomic resolution. Whoever wants to take it pays for the deinstallation, shipping and installation. The TEM locates in Milpitas, CA 95035. Contact info: Jeff (Shaojie) Wang shaojiew-at-nanolab1.com
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Title-Subject: [Filtered] Still Hunting RCA EMT EM TABLETOP Microscope Lore, Photos installed sites spares and etc...
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Please drop us a note off list We have not given up... Ed Sharpe Archivist for SMECC - Glendale AZ
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I'm trying to find out how the contribution of a hole free or Volta phase p= late to the PCTF is described mathematically in various packages that are u= sed for cryo-EM of macromolecules.
The physics behind it would also be of interest.
Does anybody know a reference or have some material that can be shared?
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Hi. Thanks for the paper. If I read it correctly they simply model the phase plate as a constant phase shift (for all spatial frequencies above the cut-on). There's also a paper by Danev where it's modeled as "1 minus a Gaussian with a standard deviation given by the radius of the spot" (charge spot, I assume). I'm not sure about either of them, since a simple charge spot doesn't lead to a projected potential that looks like that. A very specific distribution of positive and negative charges would be required.
Are there any results on what the projected potential really looks like? And what models are actually used for CTF-fitting images taken with phase plate?
All the best,
Philip
X-from: Sacha De Carlo {sacha.decarlo-at-dectris.com} Sent: 27 May 2019 15:10:48 To: Philip Köck Cc: microscopy-at-microscopy.com; 3dem-at-ncmir.ucsd.edu
Is the phase shift due to the PP treated as constant (relative to the unscattered beam) above 20 Angstrom resolution (and as unknown at very low resolution)?
Philip
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Hope to see you there.
Aya Takase . Senior Scientist Rigaku Americas Corporation 9009 New Trails Drive . The Woodlands, TX 77381 USA T: 281-362-2300 ex 208 . F: 281-364-3628
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From ronasyre474ip-at-gmail.com Tue May 28 18:04:44 2019 Return-Path: {ronasyre474ip-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.162.209] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x4SN4g86012269 for {microscopylistserverarchive7-at-microscopy.com} ; Tue, 28 May 2019 18:04:43 -0500 Received: from smtp.mixedthings.net ([180.210.99.158]) by external.newsubdomain.com with ASMTP; Wed, 29 May 2019 10:52:57 +1200 Message-ID: {DE5721A0.ABE5A249-at-gmail.com}
Dear colleagues,
TLDR (too long, didn't read): Does anyone have a suggestion for how to remove a 50nm carbon coating OR reduce FIB charging when milling on a rock sample, by other means than carbon coating?
We have an experiment where we would like to measure Raman spectra on a rock sample after milling some holes in it with a Focused Ion Beam (FIB). Unfortunately, we are observing heavy charging when imaging/milling with the ion beam. We carbon coated a sample with a 50nm carbon layer, which totally removed the charging problem, but introduced a new problem - the raman spectra are severely impaired by the carbon.
Can anyone suggest a way around this? We would like to either remove the carbon coating in a gentle manner, or find a way to reduce FIB charging without impairing the Raman.
We have many samples we would like to image in this manner and the opportunity to experiment with different suggestions.
One possibility - no guarantee to work - is to use a sharpie marker or highlighter pen instead of carbon coating. A new sharpie that is very wet will wick out a thin layer past the tip of the pen, that *might* be thin enough and conductive enough to FIB through. Touching the Omniprobe to the ink might give you enough grounding to FIB at a low beam current. A plasma cleaner that is operating correctly will remove sharpie ink after ~15 minutes.
Chad
-------------------------------- Chad M. Parish, Ph.D. Research and Development Staff Member Fundamentals of Radiation Effects Group Materials Science and Technology Division Oak Ridge National Laboratory Phone: 1 865 574 0092 Email: parishcm-at-ornl.gov Web: https://www.ornl.gov/staff-profile/chad-m-parish
-----Original Message----- X-from: thomasaarholt-at-gmail.com {thomasaarholt-at-gmail.com} Sent: Wednesday, May 29, 2019 5:06 AM To: Parish, Chad M. {parishcm-at-ornl.gov}
Dear colleagues,
TLDR (too long, didn't read): Does anyone have a suggestion for how to remove a 50nm carbon coating OR reduce FIB charging when milling on a rock sample, by other means than carbon coating?
We have an experiment where we would like to measure Raman spectra on a rock sample after milling some holes in it with a Focused Ion Beam (FIB). Unfortunately, we are observing heavy charging when imaging/milling with the ion beam. We carbon coated a sample with a 50nm carbon layer, which totally removed the charging problem, but introduced a new problem - the raman spectra are severely impaired by the carbon.
Can anyone suggest a way around this? We would like to either remove the carbon coating in a gentle manner, or find a way to reduce FIB charging without impairing the Raman.
We have many samples we would like to image in this manner and the opportunity to experiment with different suggestions.
-------- Forwarded Message -------- X-from: John Minter {jrminter-at-gmail.com}
Thomas, my lab had an older plasma asher that used Ar. It worked OK. We got a Gatan Solarus plasma asher that could use a H2/O2 gas mixture to remove C. It worked well. We used it to remove C contamination from Si chips to mount nanoparticles for high resolution SEM and to remove residual polymer/organics from the nanoparticles. Like and technique that perturbs a specimen, I would suggest running control rock samples with and without ashing to see how your Raman spectra changed.
Best regards, John Minter Retired from Kodak Analytical Sciences
Monday: Techniques and Instrumentation Tuesday: Environmental and Industrial Microscopy Wednesday: Chemical and Forensic Microscopy Thursday-Friday: Workshop: Fungal Spore Identification
Tony ……………………………………………………………….. Andrew Anthony “Tony” Havics, CIH, PE Environmental, Health & Safety, Microscopy, Materials Science & Forensic Engineering pH2, LLC 5250 E US Highway 36, Suite 830 Avon, IN 46123 (317) 718-7020 Office (317) 718-7038 Fax (317) 409-3238 Cell aahavics-at-pH2LLC.com www.ph2LLC.com
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Carbon coating is fairly easy to remove with Oxygen or Oxigen/Helium plasma. Dedicated plasma cleaner would work the best, but you can even try Evactron or IBSS in-situ cleaners. Plasma cleaner is preferred over plasma etcher, as it typically works with much lower RF power and therefore provides "gentler" cleaning.
For completely chemical removal of carbon coating get a UV cleaner (or find a lab which has it) - the removal would be terribly slow (hours or even couple of days for 50nm C removal), but absolutely no physical damage to the surface.
Easily-removable coating for preventing FIB charging would be to spray-on of one of conductive polymer, either with regular atomizer or better yet with ultrasonic nozzle. Plenty of formulations, I've worked with aquaSAVE (removable by water) and E-Shield (removable by alcohol) with great success (although never tried them on the rock samples).
Best Wishes, Valery
Valery Ray ============================== PBS&T, MEO Engineering Company 290 Broadway, Suite 298 Methuen, MA 01844, USA Mobie: +1-978-305-0479 - leave a message https://www.linkedin.com/in/valeryray/ E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On Wednesday, May 29, 2019, 5:00:33 AM EDT, thomasaarholt-at-gmail.com {thomasaarholt-at-gmail.com} wrote:
Dear colleagues,
TLDR (too long, didn't read): Does anyone have a suggestion for how to remove a 50nm carbon coating OR reduce FIB charging when milling on a rock sample, by other means than carbon coating?
We have an experiment where we would like to measure Raman spectra on a rock sample after milling some holes in it with a Focused Ion Beam (FIB). Unfortunately, we are observing heavy charging when imaging/milling with the ion beam. We carbon coated a sample with a 50nm carbon layer, which totally removed the charging problem, but introduced a new problem - the raman spectra are severely impaired by the carbon.
Can anyone suggest a way around this? We would like to either remove the carbon coating in a gentle manner, or find a way to reduce FIB charging without impairing the Raman.
We have many samples we would like to image in this manner and the opportunity to experiment with different suggestions.
My colleagues and I would like to bring to your attention the YouTube channel "M*N: Microscopy, Machine Learning, Materials" dedicated to the applications of big data methods, machine learning, and artificial intelligence in Scanning Probe Microscopy and Scanning Transmission Electron Microscopy. The channel is available at:
We aim to create the vibrant environment for sharing recent advances in this rapidly growing field, as well as to cover some of the historical developments over last decade and notable achievements in (potentially) related fields. The channel will start with 7-lecture overview of some of the developments over the last decade, including: 1. Unsupervised learning in Scanning Probe Microscopy: Spectroscopies 2. Supervised learning in Scanning Probe Microscopy: Spectroscopies 3. Linear unmixing: basic techniques and some applications in microscopy and spectroscopy 4. Supervised and unsupervised learning in Scanning Probe Microscopy: Imaging 5. Supervised and Unsupervised Learning in Scanning Transmission Electron Microscopy 6. Learning Physics (and Chemistry) from Scanning Transmission Electron Microscopy 7. Atomic fabrication by STEM: feedback, compressed sensing, and non-rectangular beam paths
The lectures will be posted weekly (or close to it), with the first one being posted on-line today.
After this initial round of lectures, we plan to post lecture tutorials on recent developments in the field of big data, machine learning, and artificial intelligence in electron, scanning probe microscopy and chemical imaging, with the lectures being planned on: - Deep learning in Scanning Transmission Electron Microscopy - Deep Learning in Scanning Tunneling Microscopy - Workflows for creation of STEM image libraries and their applications - and many more
These lectures are closely tied to the on-line data and code resources. The codes are (or will be) shared via the PyCroscopy domain on the GitHub. https://github.com/pycroscopy/pyCroscopy
The libraries of STEM images are disseminated via the CITRINation platform.
Finally, the imaging tools can accessed via the Center for Nanophase Materials Sciences. https://www.ornl.gov/facility/cnms
Subscribe, join, and stay tuned!
Sergei V. Kalinin
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Does anyone freeze small aliquots of uranyl formate for storage? Our facility has recently increased it's use of uranyl formate for negative staining but, once prepared, it has a very short shelf life. This means we are generating a lot of waste. I have seen references for freezing small aliquots but, have not used thawed UF myself. I am wonder what other's experiences have been? Do you have a strong opinion on the efficacy or quality of thawed UF as a negative stain?
Thank you, Charlene Wilke
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Email: unocicrr-at-ornl.gov Name: Raymond Unocic
Organization: Oak Ridge National Laboratory
Title-Subject: [Filtered] Postdoctoral Position at ORNL on in situ microscopy/atomic manipulation
Message: New postdoc position -at- the Center for Nanophase Materials Sciences Oak Ridge National Laboratory. Interested candidates please check out the description in the following link:
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Title-Subject: [Filtered] In-situ TEM Postdoctoral Research Associate at UConn
Message: Job Posting Title: Postdoctoral Research Associate Position on In-situ (Scanning) Transmission Electron Microscopy
The University of Connecticut (UConn), one of the top 20 public universities in the nation, invites applications for a Postdoctoral Research Associate position in the Department of Materials Science and Engineering (MSE) in the School of Engineering and the Institute of Materials Science (IMS). Applicants with a strong background in (scanning) transmission electron microscopy ((S)TEM) and in-situ experiment experiences are encouraged to apply. In this position, you will have the opportunity to engage creative research on investigating structure-property dynamics in advanced functional and structural materials including but not limited to heterogeneous catalysts. You will have the opportunity to work in a state-of-the-art TEM center hosting a probe-corrected Titan Themis STEM, and perform in-situ (gaseous) environmental microscopy at our latest InToEM center (https://today.uconn.edu/school-stories/intoem/). UConn is entering a transformational period of growth supported by the $1.7B Next Generation Connecticut (http://nextgenct.uconn.edu/) and the $1B Bioscience Connecticut (http://biosciencect.uchc.edu/) investments and a bold new Academic Plan: Path to Excellence (http://issuu.com/uconnprovost/docs/academic-plan-single-hi-optimized_1). As part of these initiatives, the new UConn-Thermo Fisher Scientific Center for Advanced Microscopy and Materials Analysis (CAMMA) has acquired seven new electron beam instruments including state-of-the-art TEM, SEM and FIB systems. These are housed in the UConn Technology Park as part of the purpose-built Advanced Characterization Laboratory, which opened earlier this year.
DUTIES AND RESPONSIBILITIES The successful candidate will share a deep commitment to transmission electron microscopy. Working under the supervision of Prof. Yuanyuan Zhu, the candidate will be expected to lead microscopy researches to further the understanding of materials dynamics. The candidate will also contribute to TEM sample preparation, mentor graduate and undergraduate students; write research proposals and progress reports; interact with research collaborators; prepare and maintain lab equipment and supplies, submit and publish peer reviewed journal papers. MINIMUM QUALIFICATIONS An earned doctorate in Materials Science, Chemistry, Physics or a related discipline. A strong background and extensive research experience in TEM sample preparation (e.g. FIB liftout) and (scanning) transmission electron microscopy characterization. Good written and verbal communication skills. Good research capabilities as evidenced by a record of publication of results in peer-reviewed journals and external presentations at scientific conferences. PREFERRED QUALIFICATIONS Additionally, a strong background in one or several of these fields is desirable: solid state physics, heterogeneous catalysis, surface science. The candidate is expected to be proficient at three or more of the following techniques including but not limited to: SAED, Nanobeam Electron Diffraction, HRTEM, probe-corrected STEM, EDS mapping, core- (and low-) loss EELS, in-situ heating TEM. Skills and experience in in-situ gas-cell microscopy is highly desired. Strong interpersonal skills including the ability to interact effectively with staffs, students and collaborators. APPOINTMENT TERMS The selected candidate is expected to start in August 2019 (earlier start is possible). This is a full-time (12-month appointment) position, and is renewable every year. The successful candidate’s primary academic appointment will be at the UConn main campus in Storrs, CT. Salary will commensurate with qualifications and experience. TO APPLY Please submit the following: a cover letter; curriculum vitae (with a full list of publication), copies of two representative publications to yuanyuan.2.zhu-at-uconn.edu, with a subject title “In-situTEMPostdoc_yourname”. Evaluation of applicants will begin immediately and continue until the position is filled. Employment of the successful candidate will be contingent upon the successful completion of a pre-employment criminal background check. All employees are subject to adherence to the State Code of Ethics, which may be found at http://www.ct.gov/ethics/site/default.asp. ____________________________________________________________________ The University of Connecticut is committed to building and supporting a multicultural and diverse community of students, faculty, and staff. The diversity of students, faculty, and staff continues to increase, as does the number of honors students, valedictorians and salutatorians who consistently make UConn their top choice. More than 100 research centers and institutes serve the University’s teaching, research, diversity, and outreach missions, leading to UConn’s ranking as one of the nation’s top research universities. UConn’s faculty and staff are the critical link to fostering and expanding our vibrant, multicultural, and diverse community. As an Affirmative Action/Equal Employment Opportunity employer, UConn encourages applications from women, veterans, people with disabilities, and members of traditionally underrepresented populations.
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Email: mnahmani-at-uw.edu
Name: Marc Nahmani
Organization: UW Tacoma
Title-Subject: [Filtered] Help Moving and Reassembling a Zeiss LSM510 Confocal
Message: I'm moving a Zeiss LSM510 inverted Confocal and laser module from Missouri to Washington state. I'm looking to hire someone experienced in Zeiss confocals to help pack and reassemble the scope in my lab in WA. Anyone fit this description or have recommendations?
Thanks in advance! Marc
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Dear Charlene, Yes we do store our uranyl formate in 300 ml aliquots in a -80 freezer. We thaw once just before use (cold running water). The solution has already been filtered (0.22 um). We have never had any problems.
Good luck, Michael Delannoy Johns Hopins SOM Microscope Facility.
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X-from: c-wilke-at-northwestern.edu
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Does anyone freeze small aliquots of uranyl formate for storage? Our facility has recently increased it's use of uranyl formate for negative staining but, once prepared, it has a very short shelf life. This means we are generating a lot of waste. I have seen references for freezing small aliquots but, have not used thawed UF myself. I am wonder what other's experiences have been? Do you have a strong opinion on the efficacy or quality of thawed UF as a negative stain?
Thank you, Charlene Wilke
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We've had to move all of our instruments, including SEM and confocals, twice in 3 years, and got the vendors (Zeiss, Leica and Bruker) to do most of the moving, from packing up to reassembly. Probably more expensive but that way any damage is covered and the instrument is set up correctly in the new location. There are a couple of local expert engineers, and one of these helped also. Worth getting a quote from Zeiss.
cheers, Rosemary
Dr Rosemary White CSIRO Black Mountain GPO Box 1700 ACT 2601, Australia
P: 61-2-62465475 E: rosemary.white-at-anu.edu.au ________________________________________ X-from: microscopy.listserver-at-gmail.com [microscopy.listserver-at-gmail.com] Sent: Tuesday, 4 June 2019 11:27 PM To: Rosemary White
X-from: mnahmani-at-uw.edu
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Email: mnahmani-at-uw.edu
Name: Marc Nahmani
Organization: UW Tacoma
Title-Subject: [Filtered] Help Moving and Reassembling a Zeiss LSM510 Confocal
Message: I'm moving a Zeiss LSM510 inverted Confocal and laser module from Missouri to Washington state. I'm looking to hire someone experienced in Zeiss confocals to help pack and reassemble the scope in my lab in WA. Anyone fit this description or have recommendations?
Thanks in advance! Marc
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X-from: Diane.M.Curley-at-KCC.com
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Email: Diane.M.Curley-at-KCC.com Name: Diane M, Curley, PhD
Organization: Kimberly-Clark
Title-Subject: [Filtered] For Donation: Cargille DisCups
Message: Cargille DisCups 120cc graduated paper beakers for embedding media 2000 cups total, still protected in plastic sleeves
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Email: rinnes-at-indiana.edu Name: Roger Innes
Organization: Indiana University Title-Subject: [Filtered] Two Ph.D. level EM Staff Positions at Indiana University Message: The Indiana University Bloomington Electron Microscopy Center (http://iubemcenter.indiana.edu/) is expanding and has openings for two full time staff positions. One position is focused on Materials Science (https://indiana.peopleadmin.com/postings/7955) and the second is focused on Cryo-Electron Microscopy (http://indiana.peopleadmin.com/postings/7192). Questions concerning these positions may be addressed to Roger Innes (rinnes-at-indiana.edu), Director of the IUB-EMC. IU Bloomington is located in the rolling hills and hardwood forests of southern Indiana. Bloomington is a liberal university town with a vibrant music and arts scene, but without the crowds and traffic of a large city. Indianapolis, Cincinnati, St. Louis and Chicago are all within a four-hour drive.
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Email: kpseverin-at-alaska.edu Name: Ken Severin
Organization: University of Alaska Fairbanks
Title-Subject: [Filtered] Quanta 200 boards
Message: As happens all too often these days I'm stuck with an SEM that is mechanically in great shape but a proprietary board (the ViperQuad) has died and it is horribly expensive from FEI. The instrument is an older Quanta 200 with the WIN2000 operating system for the microscope itself. If anyone has a ViperQuad board (yes, they are on ebay but don't have the FEI mod) or a suggestion for some other workaround, I'm all ears.
Thanks much!
Ken Severin - U Alaska Fairbanks kpseverin-at-alaska.edu
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I need to make some permanent labels for my teaching slide sets. Does anyone have a label maker and tape recommendation. I am particularly interested in tape that resists water and smudging from handling. I want a long-lasting durable label. Thanks, Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Curators' Distinguished Teaching Professor Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
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X-from: jabees2003-at-hotmail.com
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Message: I am Dr. Ayaz Ahmed, working as an Assistant Professor at the International Center for Chemical and Biological Sciences (ICCBS), University of Karachi.
I have 90i Microscopy installed in our lab but recently I am facing a problem that its connectivity with the computer is lost and it displayed device communication error.
Rest of the microscope working fine but it is not connected with the software installed. With very best regards
Dr. Ayaz Ahmed Assistant Professor PCMD - ICCBS University of Karachi
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Email: desertrat99-at-verizon.net
Name: Eric Rosen
Organization: UCLA
Title-Subject: [Filtered] Hot plates. Message: Hi,
Our lab is in need of some new hot plates for drying slides for Thick sections and for drying ultrathins on the grids.
Currently, we have some Thermolyne Nuova hot plates, but are looking for a similar replacement. The good thing about the current hot,plates is they have a area we can place the tweezers on that they do not get heated but have the grids over the hot plate itself so, the thin sections will down onto the grids. Do,you have any recommendations for hot plates? I have been looking but not found anything similar that is from an approved vendor I can purchase from. Thanks. Login Host: 149.142.103.136 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
To create absolutely permanent, un-removable making on glass slides just Google for a machine shop offering laser making in your area. The shop doesn't necessary have to be currently working with glass - most laser marking machines would etch glass. Chances are, local hacker-space in your area, or some lab at your University has pulsed laser ablation or laser marking system and could do the marking for you.
If you have a steady hand, then $15 micro-engraver from Amazon with diamond burr would do the same permanent marking, or insert the engraver into pantorgraph or CNC router...
Valery
Valery Ray ============================== PBS&T, MEO Engineering Company 290 Broadway, Suite 298 Methuen, MA 01844, USA Mobie: +1-978-305-0479 - leave a message https://www.linkedin.com/in/valeryray/ E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On Wednesday, June 12, 2019, 10:09:43 AM EDT, PhillipsT-at-missouri.edu {PhillipsT-at-missouri.edu} wrote:
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor:Â The Microscopy Society of America
I need to make some permanent labels for my teaching slide sets. Does anyone have a label maker and tape recommendation. I am particularly interested in tape that resists water and smudging from handling. I want a long-lasting durable label. Thanks, Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Curators' Distinguished Teaching Professor Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu {mailto:phillipst-at-missouri.edu}
I second the engraving option (manual on my end - I don’t have access to a laser engraving system… yet. Definitely the most resistant one (although it takes a little practice for a proper engraving by hand with a diamond or tungsten carbide pen…).
Several years ago, I had several samples given to my old university collection with sample numbers written with a high quality permanent marker on the glass slide, and the inscription covered with a varnish or some kind of glue / epoxy. I don’t remember what varnish / glue they were using but it was like a kind of clear nail polish. Not sure on the very long term (} 50 years) how it would hold (and not crack / flake), and the ink might actually diffuse into the epoxy, creating a blurry purplish effect, and do a nice chromatographic analysis of the ink over the decades???
Other solution that we employ when making epoxy blocks or thin sections (of rock...) with epoxy / glue is to print a small label and embed this label on the side of the thin section.
However, the last two solutions applies well for rocks / mineral sample preparation or samples that are embedded in epoxy, not sure if this would work in your field…
Best,
Julien
########################### *Dr. Julien Allaz *Head assistant for SEM/EPMA Inst. fĂĽr Geochemie und Petrologie ETH ZĂĽrich NWÂ E 84 Clausiusstrasse 25 8092Â ZĂĽrich Switzerland
X-from: Cromey, Douglas W - (dcromey) {dcromey-at-email.arizona.edu}
Thomas,
You might check with the histopathology lab of a nearby hospital. If you like what they use, perhaps you could work out an arrangement with the lab and save yourself the trouble/expense of purchasing a high-end label printer.
Their slides often need to be stored for decades, so your interests and theirs are similar.
Doug
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ Douglas W. Cromey, M.S. - Associate Scientific Investigator Dept. of Cellular & Molecular Medicine, University of Arizona 1501 N. Campbell Ave, Tucson, AZÂ 85724-5044 USA
office:Â LSN 463 Â Â Â Â email: dcromey-at-email.arizona.edu voice:Â 520-626-2824Â Â Â Â Â Â fax:Â 520-626-2097
http://swehsc.pharmacy.arizona.edu/micro Home of: "Microscopy and Imaging Resources on the WWW"
UA Microscopy Alliance -Â http://microscopy.arizona.edu/
-----Original Message----- X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Thursday, June 13, 2019 6:34 AM To: Cromey, Douglas W - (dcromey) {dcromey-at-email.arizona.edu}
To create absolutely permanent, un-removable making on glass slides just Google for a machine shop offering laser making in your area. The shop doesn't necessary have to be currently working with glass - most laser marking machines would etch glass. Chances are, local hacker-space in your area, or some lab at your University has pulsed laser ablation or laser marking system and could do the marking for you.
If you have a steady hand, then $15 micro-engraver from Amazon with diamond burr would do the same permanent marking, or insert the engraver into pantorgraph or CNC router...
Valery
Valery Ray ============================== PBS&T, MEO Engineering Company 290 Broadway, Suite 298 Methuen, MA 01844, USA Mobie: +1-978-305-0479 - leave a message https://www.linkedin.com/in/valeryray/ E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On Wednesday, June 12, 2019, 10:09:43 AM EDT, PhillipsT-at-missouri.edu {PhillipsT-at-missouri.edu} wrote:
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor:Â The Microscopy Society of America
I need to make some permanent labels for my teaching slide sets. Does anyone have a label maker and tape recommendation. I am particularly interested in tape that resists water and smudging from handling. I want a long-lasting durable label. Thanks, Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Curators' Distinguished Teaching Professor Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu {mailto:phillipst-at-missouri.edu}
For those of you out there doing single particle analysis with Cryo TEM, about how many hours to do a single sample from start of processing the sample to final data collection off the microscope?
Tom Bargar UNMC Electron Microscopy Core Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
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Email: desertrat99-at-verizon.net Name: Eric Rosen
Organization: UCLA
Title-Subject: [Filtered] Hot plates. Message: Hi, Our lab is in need of some new hot plates for drying slides for Thick sections and for drying ultrathins on the grids. Currently, we have some Thermolyne Nuova hot plates, but are looking for a similar replacement. The good thing about the current hot,plates is they have a area we can place the tweezers on that they do not get heated but have the grids over the hot plate itself so, the thin sections will down onto the grids. Do,you have any recommendations for hot plates? I have been looking but not found anything similar that is from an approved vendor I can purchase from. Thanks. Login Host: 149.142.103.136 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
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Organization: UMass Amherst and Harvard University
Title-Subject: [Filtered] pyrogallol and OsO4 in biological tissue
Message: Does anyone know what exactly occurs between pyrogallol and osmium tetroxide when used in EM processing of biological tissue? Is it similar to the ROTO/rOTO (reduced osmium-TCH-osmium) process, in which greater deposition of osmium occurs via TCH (thiocarbohydrazide)? I am looking to chat with anyone with particular knowledge regarding these chemicals and EM. Login Host: 140.247.107.57 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
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Hi all.
I've been wondering what the mean inner electrostatic potential of a solid (for example the 10 V of carbon) is actually due to. Is it purely caused by the distribution of nuclei and electrons in the solid itself or could there be a contribution from adsorbed surface charges?
All the best,
Philip
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Title-Subject: [Filtered] Postdocroral position available at Northwestern University
Message: Dear Colleagues, We are looking for a postdoctoral research associate in the broad area of “Characterizing Structure and Dynamics of Soft Matter with multimodal Electron Microscopy”. The candidate will have access to state-of-the-art instrumentation for the development and application of new and unconventional approaches for Soft Matter studies. Objects of interest include engineered proteins, megamolecules, protein complexes, Metal-Organic and Covalent Frameworks (MOFs/COFs) and soft-hard interfaces. The candidate will work in the group of Professor Vinayak Dravid in the Department of Materials Science & Engineering as member of an extensive cross-disciplinary and collaborative project across Chemistry, Biomedical Engineering, and Cell & Molecular Biology experts at Northwestern and University of Chicago. The candidate will work closely with the NUANCE Center (NSF Center for Facility Excellence in the greater Midwest), which houses comprehensive sample preparation and atomic-nanoscale characterization capabilities for soft, hard and hybrid structures. In this vibrant environment, the candidate will have ample opportunity to learn diverse aspects of synthetic biology, simulation and imaging of unconventional molecules/materials. Select experiments will be performed with fluidic-cell scanned probe microscopy (FC-SPM) to probe dynamic conformation changes and molecule interactions. The preferred candidate will have a doctoral degree in live science or engineering fields. The preferred candidate will also have a proven track-record in the areas of advanced electron microscopy of biomolecules and/or soft matter. Experience should include hands-on experience in electron microscopy, in image processing, and in data-processing for the reconstruction of sample structures. Please send applications with introduction letter, CV, research statement (1 page), and contact information for 3 references (name, postal & email address, phone number) as a single PDF to amy.morgan-at-northwestern.edu and v-dravid-at-northwestern.edu.
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Thanks for the reference.
I can't make sense of a thickness-independent contribution to the phase shift either. The way I see it even a surface layer of dipoles would lead to a constant MIP and a phase shift proportional to the thickness.
One can think of a simple model: A slab of completely neutral material (made of neutrons) covered in a layer of positive charge and outside that a layer of negative charge that balances the positive charge. The potential inside this slab will be constant and independent of the thickness of the slab.
I wonder if we can get a comment from someone who knows more.
All the best,
Philip
X-from: 3dem {3dem-bounces-at-ncmir.ucsd.edu} on behalf of Benjamin Himes {himes.benjamin-at-gmail.com} Sent: Monday, 17 June 2019 20:45:48 To: 3dem-at-ncmir.ucsd.edu
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Email: nasadi-at-ufl.edu Name: Navid Asadi
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Title-Subject: [Filtered] 2019 PAINE conference
Message: Call for participation 2019 PAINE conference
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Email: raischt-at-vcu.edu Name: Tristan Raisch
Organization: Virginia Commonwealth University
Title-Subject: [Filtered] Cell transfer from PDMS for TEM
Message: Hi,
I'm trying to utilize a combination cell stretcher/electrical stimulator to induce certain behaviors in iPSC-derived cardiomyocytes. The system uses PDMS wells to stretch 2D cell monolayers, but I also want to see how said system impacts intercalated disc ultrastructure. Does anyone have any idea how I might transfer a cell monolayer from the PDMS wells onto a substrate that can be prepped for TEM? The kicker: I can't use trypsin or any other enzyme that digests cell/ECM contacts, as that will also disrupt the structures I want to image. I also don't want to damage the very expensive PDMS wells.
Any ideas? Thanks!
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If the well is not too small, you could briefly fix the cells with glutaraldehyde** and use a small cell scraper (you can search Google for a supplier) to remove the cells from the bottom of the PDMS wells. Use a pipet to transfer cells to a microfuge tube, spin to make a firm pellet, let fix for 1 hr and replace the glutaraldehyde with buffer, twice (trying to not disturb the pellet). Fix in osmium, rinse, embed in 2% Agar (you will need to stir the cells a bit), refrigerate until the Agar is hardened, remove the plug of Agar, cut into smaller pieces that contain the cells, and process for TEM. If there are not enough cells to make a visible pellet, you could combine the cells from several wells.
**The glutaraldehyde fix should help maintain the ultrastructure of the intercalated discs.
I hope you find this helpful.
Best regards, Cynthia
Cynthia Goldsmith, MGS Infectious Diseases Pathology Branch Centers for Disease Control and Prevention (CDC) Atlanta, GA 30329 (404) 639-3306
-----Original Message----- X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Tuesday, June 18, 2019 4:34 AM To: Goldsmith, Cynthia (CDC/DDID/NCEZID/DHCPP) {csg1-at-cdc.gov}
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Email: raischt-at-vcu.edu Name: Tristan Raisch
Organization: Virginia Commonwealth University
Title-Subject: [Filtered] Cell transfer from PDMS for TEM
Message: Hi,
I'm trying to utilize a combination cell stretcher/electrical stimulator to induce certain behaviors in iPSC-derived cardiomyocytes. The system uses PDMS wells to stretch 2D cell monolayers, but I also want to see how said system impacts intercalated disc ultrastructure. Does anyone have any idea how I might transfer a cell monolayer from the PDMS wells onto a substrate that can be prepped for TEM? The kicker: I can't use trypsin or any other enzyme that digests cell/ECM contacts, as that will also disrupt the structures I want to image. I also don't want to damage the very expensive PDMS wells.
Any ideas? Thanks!
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You might try growing the cells on Permanox petri plates. These plastic plates are 60mm in diameter, not too very large, and they are compatible with Spurr's plastic resins all the way through the process (no need to use propylene oxide. In fact DO NOT use PO as it will etch the plate). The plate will separate easily from the plastic dish while still warm from the embedding oven. You can select the area you want for ultra-microtomy by examining the cell "cookie" under a light microscope. The plates are available from Electron Microscopy Sciences in sterile bags of 40. If you'd like more info, please feel free to contact me at debrat-at-bcm.edu.
Cheers, Debra
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Tuesday, June 18, 2019 3:31 AM To: Townley, Debra
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Email: raischt-at-vcu.edu Name: Tristan Raisch
Organization: Virginia Commonwealth University
Title-Subject: [Filtered] Cell transfer from PDMS for TEM
Message: Hi,
I'm trying to utilize a combination cell stretcher/electrical stimulator to induce certain behaviors in iPSC-derived cardiomyocytes. The system uses PDMS wells to stretch 2D cell monolayers, but I also want to see how said system impacts intercalated disc ultrastructure. Does anyone have any idea how I might transfer a cell monolayer from the PDMS wells onto a substrate that can be prepped for TEM? The kicker: I can't use trypsin or any other enzyme that digests cell/ECM contacts, as that will also disrupt the structures I want to image. I also don't want to damage the very expensive PDMS wells.
Any ideas? Thanks!
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Organization: University of Arizona
Title-Subject: [Filtered] Postdoctoral Research Position in Planetary Materials at the University of Arizona
Message: The Lunar and Planetary Laboratory at the University of Arizona invites applications for a postdoctoral research position in plantery materials and electron microscopy. Position Summary from the website:
The Lunar and Planetary Laboratory of the University of Arizona (https://www.lpl.arizona.edu) invites applications for a postdoctoral researcher in the fields of electron microscopy and planetary materials. The successful applicant will be part of the planetary materials research group (https://www.lpl.arizona.edu/PMRG/) that uses electron microscopy to explore the nature and origins of planetary materials. The initial appointment is for one year with expected renewal up to three years, pending funding and satisfactory performance. The position is expected to begin January of 2020.
The University of Arizona has recently invested in electron microscopy, and the postdoctoral candidate will have access to a Cameca SX-100 electron microprobe, two Hitachi scanning electron microscopes, a ThermoScientific Helios focused-ion-beam scanning-electron microscope, and a 200 keV aberration-corrected Hitachi HF5000 scanning transmission electron microscope. These instruments are housed within the Kuiper Materials Imaging and Characterization Facility and will play a critical role in the analysis of samples to be returned by the NASA OSIRIS-REx Mission being led by the Lunar and Planetary Laboratory at the University of Arizona.
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From normanatlas54uinyu-at-gmail.com Wed Jun 19 04:32:12 2019 Return-Path: {normanatlas54uinyu-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.162.207] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x5J9WBbB027825 for {microscopylistserverarchive7-at-microscopy.com} ; Wed, 19 Jun 2019 04:32:12 -0500 Received: from unknown (HELO mail.naihautsui.co.kr) (Wed, 19 Jun 2019 19:25:32 +1000) by smtp.endend.nl with ESMTP; Wed, 19 Jun 2019 19:25:32 +1000 Received: from mts.locks.grgtween.net ([117.162.135.188]) by qrx.quickslick.com with QMQP; Wed, 19 Jun 2019 19:08:42 +1000 Received: from unknown (HELO webmail.halftomorrow.com) (Wed, 19 Jun 2019 18:50:58 +1000) by m1.gns.snv.thisdomainl.com with SMTP; Wed, 19 Jun 2019 18:50:58 +1000 Message-ID: {58BED0F2.82822881-at-gmail.com}
} On Jun 17, 2019, at 1:27 AM, Philip Köck {philip.koeck-at-ki.se} wrote: } } } Hi all. } } I've been wondering what the mean inner electrostatic potential of a solid (for example the 10 V of carbon) is actually due to. } Is it purely caused by the distribution of nuclei and electrons in the solid itself or could there be a contribution from adsorbed surface charges? } } All the best, } } Philip } } } } När du skickar e-post till Karolinska Institutet (KI) innebär detta att KI kommer att behandla dina personuppgifter. Här finns information om hur KI behandlar personuppgifter {https://ki.se/medarbetare/integritetsskyddspolicy} . } } } Sending email to Karolinska Institutet (KI) will result in KI processing your personal data. You can read more about KI’s processing of personal data here {https://ki.se/en/staff/data-protection-policy} . } _______________________________________________ } 3dem mailing list } 3dem-at-ncmir.ucsd.edu } https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem
Hi Philip, Since each material has an unambiguous value for MIP, I would think that adsorbed surface charges are not included, since these can vary. Yours, Bill
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Dear All, We have had a Q150T ES sputter-coater from Quorum Technologies Ltd since 2014. It was working fine up to the last Friday. The problem is in the rotary table. It is not rotating now. If the "Run stage" profile is run, the table is not rotating. In the service log, there is no error message, but standard output: "Run stage - Run stage Process completed without errors".
The table is not rotating during standard sputtering processes too and also without any error log.
Please, did anybody have such problems with Q150T ES?
Thanking you in advance for any response.
My best regards
Oldrich
-- OldĹ™ich Benada Institute of Microbiology, Czech Acad. Sci. Laboratory of Molecular Structure Characterization Electron Microscopy Group VĂdeĹská 1083 142 20 Prague 4 Czech Republic
==============================Original Headers============================== 8, 25 -- From benada-at-biomed.cas.cz Mon Jun 24 04:19:43 2019 8, 25 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) 8, 25 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x5O9Jfgm015392 8, 25 -- for {microscopy-at-microscopy.com} ; Mon, 24 Jun 2019 04:19:42 -0500 8, 25 -- Received: from u117ob02 (nb170ph.mbu.cas.cz [147.231.44.133]) 8, 25 -- (using TLSv1.2 with cipher ECDHE-RSA-AES256-GCM-SHA384 (256/256 bits)) 8, 25 -- (No client certificate requested) 8, 25 -- by mail2.biomed.cas.cz (Postfix) with ESMTPSA id 3B70ED00CA0 8, 25 -- for {microscopy-at-microscopy.com} ; Mon, 24 Jun 2019 11:20:26 +0200 (CEST) 8, 25 -- Date: Mon, 24 Jun 2019 11:20:25 +0200 8, 25 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 8, 25 -- To: {microscopy-at-microscopy.com} 8, 25 -- Subject: Q150T ES =?UTF-8?B?4oCT?= sputter-coater problem 8, 25 -- Message-ID: {20190624112025.753ba741-at-u117ob02} 8, 25 -- Organization: =?UTF-8?B?TWlrcm9iaW9sb2dpY2vDvSDDunN0YXY=?= AV 8, 25 -- =?UTF-8?B?xIxS?= 8, 25 -- X-Mailer: Claws Mail 3.17.1 (GTK+ 2.24.31; i686-pc-linux-gnu) 8, 25 -- MIME-Version: 1.0 8, 25 -- Content-Type: text/plain; charset=UTF-8 8, 25 -- X-IoP-CAS-MailScanner-Information: Please contact the ISP for more information 8, 25 -- X-IoP-CAS-MailScanner-ID: 3B70ED00CA0.A8D94 8, 25 -- X-IoP-CAS-MailScanner: Processed 8, 25 -- X-Spam-Status: No 8, 25 -- Content-Transfer-Encoding: 8bit 8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id x5O9Jfgm015392 ==============================End of - Headers==============================
From ralpzand8ux-at-gmail.com Mon Jun 24 09:38:52 2019 Return-Path: {ralpzand8ux-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.162.196] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x5OEcpAg032625 for {microscopylistserverarchive7-at-microscopy.com} ; Mon, 24 Jun 2019 09:38:52 -0500 Received: from [141.162.44.203] by mail.gimmicc.net with NNFMP; Tue, 25 Jun 2019 01:34:14 +1100 Received: from smtp.doneohx.com ([Tue, 25 Jun 2019 01:32:15 +1100]) by smtp4.cyberemailings.com with ASMTP; Tue, 25 Jun 2019 01:32:15 +1100 Received: from unknown (38.197.235.103) by webmail.halftomorrow.com with ASMTP; Tue, 25 Jun 2019 01:26:35 +1100 Message-ID: {380CE608.DF8AEDBC-at-gmail.com}
The statement in Wanner et al. 2006 is very strange in my opinion. Shouldn't "perpendicular" be replaced by "parallel"?
I don't see how the potential can be proportional to the distance between the dipole layers. If we think of a slab of neutral matter (as a thought experiment), which is covered first by a layer of positive charges and then an equal amount of negative charges on top of that, the following should happen: The negative charge curves the potential upwards and the potential increases, then the positive charge curves the potential down again by an equal amount. The total effect is that the potential is constant inside the slab and independent of the slab's thickness and therefore the phase shift is proportional to the thickness. Maybe you meant that the potential is roughly proportional to the distance between the negative and positive layer (or more generally the dipole moment).
I can imagine a constant phase shift coming from multiple layers of charge, at least 3, that are balanced. A bit like - + + -, for example. That could lead to a potential that's confined to the surface and a phase shift contribution that's independent of the thickness of the solid.
All the best,
Philip
X-from: 3dem {3dem-bounces-at-ncmir.ucsd.edu} on behalf of Vladan Lucic {vladan-at-biochem.mpg.de} Sent: Wednesday, 26 June 2019 17:57:58 To: 3dem-at-ncmir.ucsd.edu Subject: Re: [3dem] (mean Inner potential) Re: 3dem Digest, Vol 142, Issue 38
I agree that classically, the potential generated by two surface layers of dipoles oriented perpendicular to the surface is in the first approximation directly proportional to the distance between the layers, that is to the thickness, which argues against the thickness-independent phase shift. Perhaps that is the reason for Wanner et al 2006 (the paper recommended by Ben) to propose that dipoles perpendicular to the beam should be considered:
"Although only dipole moments with a component perpendicular to the electron beam would contribute to a phase shift the irregular and corrugated a-C surface will provide attachment sites for H2O molecules to fulfill this condition."
Interestingly, Hettler et al 2018, Charging of carbon thin films in scanning and phase-plate transmission electron microscopy ( https://doi.org/10.1016/j.ultramic.2017.09.009 ) argue that the high surface roughness of the Volta phase plate is needed to generate the phase shift (at a high temperature). Furthermore, in their picture, surface dipoles are absent from the region of the direct beam, which causes lateral (perpendicular to the beam) redistribution of electrons. Both of these could provide the "perpendicular dipoles" proposed by Wanner et al 2006.
Vladan
On 6/18/19 9:26 AM, Philip Köck wrote:
Thanks for the reference.
I can't make sense of a thickness-independent contribution to the phase shift either. The way I see it even a surface layer of dipoles would lead to a constant MIP and a phase shift proportional to the thickness. One can think of a simple model: A slab of completely neutral material (made of neutrons) covered in a layer of positive charge and outside that a layer of negative charge that balances the positive charge. The potential inside this slab will be constant and independent of the thickness of the slab.
I wonder if we can get a comment from someone who knows more.
All the best,
Philip
När du skickar e-post till Karolinska Institutet (KI) innebär detta att KI kommer att behandla dina personuppgifter. Här finns information om hur KI behandlar personuppgifter {https://ki.se/medarbetare/integritetsskyddspolicy} .
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Job responsibilities: - Able to learn and operate Focus-Ion-Beam SEM (FIB-SEM) for 3D data acquisition and subsequent image processing - Develop projects of correlative light and electron microscopy (CLEM) - Assist investigators in viewing and imaging with transmission electron microscopes - Train students or postdocs on independent operation of electron microscopes and ancillary equipment - Support user cryo EM operation in the facility. - Assist the facility director with educational tasks (classes, workshops etc.)
Requirements: - A Master's degree in biological or physical sciences - At least 3 years of experience in biological electron microscopy - Ability to learn new skills and techniques - Ability to perform complex imaging and data processing procedures - High degree of reliability, organizational and interpersonal skills - Ability to work independently and be team-oriented - Special consideration will be given to candidates who have advanced EM expertise such as FIB-SEM and electron tomography for biological samples
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Email: christopher.arble
Name: Christopher Arble
Organization: NIST
Title-Subject: [Filtered] SEM Omniprobe Gas Delivery System Interface
Message: Hello, We have an OmniGIS system SN#GIS3019 with a Omniprobe Rev 3.0 control rack and software OmniGIS 5.7.0. The controlrack is connected to the computer with an ethernet cable and has an assigned IP that the OmniGIS program sees as the serial IP. When we launch the software, it connects to the serial IP address and then fails to connect to the motion controller giving error messages of "Bad connection RS232 and RS438". There is a file in the program folder that lists the Motion IP which even if I assign the address to it the program still fails to communicate with it. Does anyone have experience with this system and would be able to provide insight? -Chris Arble
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The log entry "Run stage - Run stage Process completed without errors" means that software routine giving a command to rotate the stage have executed. If the stage is not rotating, then most likely there is some kind of a hardware problem. Such problem could be either motor failure, or driver failure, or oxidized connector, or broken wire, or rotational mechanism stuck because of gunk and debris that have accumulated inside of the mechanism during five years of operation.
Diagnosing hardware problems remotely take quite a bit of effort - finding help from some local tech who is capable of servicing vacuum instrumentation could be much more efficient.
Best Wishes! Valery
Valery Ray (also with REFINE Lab, UCONN) ================================ PBS&T, MEO Engineering Company 290 Broadway, Suite 298 Methuen, MA 01844, USA Mobie: +1-978-305-0479 - leave a message https://www.linkedin.com/in/valeryray/ E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On Monday, June 24, 2019, 5:22:16 AM EDT, benada-at-biomed.cas.cz {benada-at-biomed.cas.cz} wrote:
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Dear All, We have had a Q150T ES sputter-coater from Quorum Technologies Ltd since 2014. It was working fine up to the last Friday. The problem is in the rotary table. It is not rotating now. If the "Run stage" profile is run, the table is not rotating. In the service log, there is no error message, but standard output: "Run stage - Run stage Process completed without errors".
The table is not rotating during standard sputtering processes too and also without any error log.
Please, did anybody have such problems with Q150T ES?
Thanking you in advance for any response.
My best regards
Oldrich
-- OldĹ™ich Benada Institute of Microbiology, Czech Acad. Sci. Laboratory of Molecular Structure Characterization Electron Microscopy Group VĂdeĹská 1083 142 20 Prague 4 Czech Republic
==============================Original Headers============================== 8, 25 -- From benada-at-biomed.cas.cz {mailto:benada-at-biomed.cas.cz} Mon Jun 24 04:19:43 2019 8, 25 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) 8, 25 -- Â Â Â by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x5O9Jfgm015392 8, 25 -- Â Â Â for {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } ; Mon, 24 Jun 2019 04:19:42 -0500 8, 25 -- Received: from u117ob02 (nb170ph.mbu.cas.cz [147.231.44.133]) 8, 25 -- Â Â Â (using TLSv1.2 with cipher ECDHE-RSA-AES256-GCM-SHA384 (256/256 bits)) 8, 25 -- Â Â Â (No client certificate requested) 8, 25 -- Â Â Â by mail2.biomed.cas.cz (Postfix) with ESMTPSA id 3B70ED00CA0 8, 25 -- Â Â Â for {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } ; Mon, 24 Jun 2019 11:20:26 +0200 (CEST) 8, 25 -- Date: Mon, 24 Jun 2019 11:20:25 +0200 8, 25 -- From: Oldrich Benada {benada-at-biomed.cas.cz {mailto:benada-at-biomed.cas.cz} } 8, 25 -- To: {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } 8, 25 -- Subject: Q150T ES =?UTF-8?B?4oCT?= sputter-coater problem 8, 25 -- Message-ID: {20190624112025.753ba741-at-u117ob02 {mailto:20190624112025.753ba741-at-u117ob02} } 8, 25 -- Organization: =?UTF-8?B?TWlrcm9iaW9sb2dpY2vDvSDDunN0YXY=?= AV 8, 25 --Â =?UTF-8?B?xIxS?= 8, 25 -- X-Mailer: Claws Mail 3.17.1 (GTK+ 2.24.31; i686-pc-linux-gnu) 8, 25 -- MIME-Version: 1.0 8, 25 -- Content-Type: text/plain; charset=UTF-8 8, 25 -- X-IoP-CAS-MailScanner-Information: Please contact the ISP for more information 8, 25 -- X-IoP-CAS-MailScanner-ID: 3B70ED00CA0.A8D94 8, 25 -- X-IoP-CAS-MailScanner: Processed 8, 25 -- X-Spam-Status: No 8, 25 -- Content-Transfer-Encoding: 8bit 8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id x5O9Jfgm015392 ==============================End of - Headers==============================
I have g\had great experience with support for Omniprobe products by folks from Nanoprobe Technical Services https://www.nanoprobetech.com/
If Carlos and Robert couldn't help - feel free to give me a call.
Happy July 4th week! Valery
Valery Ray ============================== PBS&T, MEO Engineering Company 290 Broadway, Suite 298 Methuen, MA 01844, USA Mobie: +1-978-305-0479 - leave a message https://www.linkedin.com/in/valeryray/ E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On Thursday, June 27, 2019, 2:59:31 PM EDT, {microscopy.listserver-at-gmail.com} wrote:
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X-from: christopher.arble
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both christopher.arble, Microscopy-at-Microscopy.com {mailto:Microscopy-at-Microscopy.com} so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Email: christopher.arble
Name: Christopher Arble
Organization: NIST
Title-Subject: [Filtered] SEM Omniprobe Gas Delivery System Interface
Message: Hello, We have an OmniGIS system SN#GIS3019 with a Omniprobe Rev 3.0 control rack and software OmniGIS 5.7.0. The controlrack is connected to the computer with an ethernet cable and has an assigned IP that the OmniGIS program sees as the serial IP. When we launch the software, it connects to the serial IP address and then fails to connect to the motion controller giving error messages of "Bad connection RS232 and RS438". There is a file in the program folder that lists the Motion IP which even if I assign the address to it the program still fails to communicate with it. Does anyone have experience with this system and would be able to provide insight? -Chris Arble
 Login Host: 129.6.135.120  Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
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I'm looking for advice on the use of Adobe Photoshop for image processing and measuring going forward. I have seen news regarding warnings against using old versions of the CC software, and am wondering if I should be concerned by the fact that it may only be supported/approved in its subscription version. Could it be more subject to change on short notice, and more difficult for users to keep a stable version? Could Adobe make changes that affect the way scientists use it, without affecting [what I assume is] the larger base of artistic users?
For a little background to frame my questions, our lab employs stereological tools to quantify kidney structural features using digital TEM images. We rely on the Photoshop Ruler for calibrating magnification and some measuring of features. We trust that the fundamental image pixel size, image resolution and the way counting grids are layered will not be altered. We generate large montages of TEM digital micrographs, so handling multiple 400 MB files without locking up is key. And finally, stability in appearance is important in streamlining procedures like this involving heavy user input.
Many of us like the idea of installing a certain version of Adobe Photoshop and using that version throughout the course of analyzing a complete data set (which may take years for a multi-year study).
So my question to the community - If you were about to begin a multi-year study, what application would you trust for simple manipulation and measurement of large images? Are you considering cutting the Photoshop cord, or are these concerns I have nothing but chatter and spin?
Thanks in advance!
Karen [Zaruba] Feller
Mauer Morphometry Lab University of Minnesota Pediatric Nephrology Minneapolis, MN 55455
I would suggest looking into Gatan Microscopy Suite Software. I've used it, criticized it, continue to use it for decades. It is not going away and Gatan does offer a free download version that may provide what you need. I have no commercial or other interest in Gatan, but I do find it pretty good for calibrating images and then making measurements.
You can find it under the resources/software tools on the gatan.com website.
Good luck. Roseann
Roseann Csencsits, PhD. Senior Research Scientist Gryphon Vallecitos Laboratories 6705 Vallecitos Rd. Sunol, CA 94586 925-862-4345 rcsencsits-at-belcan.com
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Hello Listers,
I'm looking for advice on the use of Adobe Photoshop for image processing and measuring going forward. I have seen news regarding warnings against using old versions of the CC software, and am wondering if I should be concerned by the fact that it may only be supported/approved in its subscription version. Could it be more subject to change on short notice, and more difficult for users to keep a stable version? Could Adobe make changes that affect the way scientists use it, without affecting [what I assume is] the larger base of artistic users?
For a little background to frame my questions, our lab employs stereological tools to quantify kidney structural features using digital TEM images. We rely on the Photoshop Ruler for calibrating magnification and some measuring of features. We trust that the fundamental image pixel size, image resolution and the way counting grids are layered will not be altered. We generate large montages of TEM digital micrographs, so handling multiple 400 MB files without locking up is key. And finally, stability in appearance is important in streamlining procedures like this involving heavy user input.
Many of us like the idea of installing a certain version of Adobe Photoshop and using that version throughout the course of analyzing a complete data set (which may take years for a multi-year study).
So my question to the community - If you were about to begin a multi-year study, what application would you trust for simple manipulation and measurement of large images? Are you considering cutting the Photoshop cord, or are these concerns I have nothing but chatter and spin?
Thanks in advance!
Karen [Zaruba] Feller
Mauer Morphometry Lab University of Minnesota Pediatric Nephrology Minneapolis, MN 55455
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As Roseann already mentioned, Gatan Microscopy Suite (also referred to as Digital Micrograph) is a very useful software package, with many plugins to help analyze images and diffraction patterns. The features and plugins are probably weighted to benefit the materials scientist more than other fields, and the imaging editing functionality is certainly basic compared to something like Photoshop.
ImageJ/Fiji is another image editing, processing, and calibration too. It's very powerful and has a seemingly unlimited number of plugins to extend the functionality. The tools are very beneficial to those in the life sciences community, and you'll find a huge number of tutorials for things like performing automated particle counting, image segmentation to select ultrastructural features, and more. It can handle multi dimensional data, large files, and pretty much anything you can throw at it. It is also very lightweight and has a portable version for easy installation on most any computer/OS. I would certainly look into ImageJ.
Good luck, Chris
On Fri, Jun 28, 2019 at 1:49 PM {zaruba.karen-at-gmail.com} wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello Listers, } } I'm looking for advice on the use of Adobe Photoshop for image processing } and measuring going forward. I have seen news regarding warnings against } using old versions of the CC software, and am wondering if I should be } concerned by the fact that it may only be supported/approved in its } subscription version. Could it be more subject to change on short notice, } and more difficult for users to keep a stable version? Could Adobe make } changes that affect the way scientists use it, without affecting [what I } assume is] the larger base of artistic users? } } For a little background to frame my questions, our lab employs } stereological tools to quantify kidney structural features using digital } TEM images. We rely on the Photoshop Ruler for calibrating magnification } and some measuring of features. We trust that the fundamental image pixel } size, image resolution and the way counting grids are layered will not be } altered. We generate large montages of TEM digital micrographs, so handling } multiple 400 MB files without locking up is key. And finally, stability in } appearance is important in streamlining procedures like this involving } heavy user input. } } Many of us like the idea of installing a certain version of Adobe Photoshop } and using that version throughout the course of analyzing a complete data } set (which may take years for a multi-year study). } } So my question to the community - If you were about to begin a multi-year } study, what application would you trust for simple manipulation and } measurement of large images? Are you considering cutting the Photoshop } cord, or are these concerns I have nothing but chatter and spin? } } Thanks in advance! } } Karen [Zaruba] Feller } } Mauer Morphometry Lab } University of Minnesota Pediatric Nephrology } Minneapolis, MN 55455 } } ==============================Original Headers============================== } 8, 38 -- From zaruba.karen-at-gmail.com Fri Jun 28 12:47:39 2019 } 8, 38 -- Received: from mail-ed1-f41.google.com (mail-ed1-f41.google.com [209.85.208.41]) } 8, 38 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x5SHlcd6020416 } 8, 38 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Jun 2019 12:47:39 -0500 } 8, 38 -- Received: by mail-ed1-f41.google.com with SMTP id k8so11741961eds.7 } 8, 38 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Jun 2019 10:48:40 -0700 (PDT) } 8, 38 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 8, 38 -- d=gmail.com; s=20161025; } 8, 38 -- h=mime-version:from:date:message-id:subject:to; } 8, 38 -- bh=0OIvOA1gdJAQVCm3bmK/mIg+tAQsvB+t+/f8itNoSAU=; } 8, 38 -- b=OAGkIGCPpYkm6Vx6+HXTGQh5QMDKZTl8aHUbyap04MnlRsP/a8hT+JuqyVJwauycaO } 8, 38 -- UcG43qcmU0/E2qCfpWOFzQCJO3dkIlDAtc/q78S8i5PndgmfBQlHA7ImlfMc7RXz3DYj } 8, 38 -- bjxYExhiJj9Lg8qAgaJBDS7buWyCeg41EjHLxfJdlUD0gKhGMgpd4x3lqi8oIWl8vyPL } 8, 38 -- cMO7dJGOq8+iaoE37nRcLSWluC0tNDwnkbMEQ1NSKHN77LVW9dB5MO1+iEpI3nYaFU6q } 8, 38 -- w85B3at0C0i1ZWysvCddinbyk+OJhe16B51Lz0jy9ZqM36NIyxLq+ofgzAWC6e++KoBD } 8, 38 -- v2UA== } 8, 38 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 8, 38 -- d=1e100.net; s=20161025; } 8, 38 -- h=x-gm-message-state:mime-version:from:date:message-id:subject:to; } 8, 38 -- bh=0OIvOA1gdJAQVCm3bmK/mIg+tAQsvB+t+/f8itNoSAU=; } 8, 38 -- b=CjkeeNg1VbfBz0n3Bmqor44My9+p/IxOYlc5HKML1pxQoM9DrcAtaGBSTIermh6p3w } 8, 38 -- VRsvX6Sn9y9+VlyUKibLDm+jAgqUegG6yqbe6v518KP6emnLJ4Fh/VLzaeD/k2tR/SES } 8, 38 -- 7YaPXBlifZME9GwN0jRnw6YXvstj1wrBpdU3t90ZxV6AKSn6iM/lWxBJurK+6mmAZXF+ } 8, 38 -- Xk6GvE2umjUTDxodhjckySnGbMdIAut9K1bjUSoECWiB1agG/Q11Z4tqrIg79vtNPGTb } 8, 38 -- Q6/rOX/vWIyM8y3SNv+tjoTV0YnJNFV/I0Jjyr53NElXxtwRY/dYXnfhjrvAr6X35Xtg } 8, 38 -- sz9A== } 8, 38 -- X-Gm-Message-State: APjAAAX1iFVZBpwTYQppqfatT3TF5F6p9MkXZkJJIaOtTrH4tvMh9Tk0 } 8, 38 -- lae4xRC28SKcUOt0G5szOcnnxYH4le1RtnJ4mNKovw== } 8, 38 -- X-Google-Smtp-Source: APXvYqxa5CLsCbWroeR/jv7SEtr0mW+v+KQXlnXTBZfzxDOyi4B03YTXy90LmXolyaIMs+glApCZzmNEEy99Vpgo3xg= } 8, 38 -- X-Received: by 2002:a50:ad45:: with SMTP id z5mr12875781edc.21.1561744120097; } 8, 38 -- Fri, 28 Jun 2019 10:48:40 -0700 (PDT) } 8, 38 -- MIME-Version: 1.0 } 8, 38 -- From: "Karen [Zaruba] Feller" {zaruba.karen-at-gmail.com} } 8, 38 -- Date: Fri, 28 Jun 2019 12:48:28 -0500 } 8, 38 -- Message-ID: {CA+xXf0BSof=1Fst0GHDLSzGTNwC9HjRK_np+DeYEPRS2jFqf0w-at-mail.gmail.com} } 8, 38 -- Subject: TEM - Photoshop or alternatives going forward } 8, 38 -- To: Microscopy-at-microscopy.com } 8, 38 -- Content-Type: text/plain; charset="UTF-8" } ==============================End of - Headers==============================
-- Transmission Electron Microscopy Lab Manager Analytical Instrumentation Facility (AIF) NC State University https://www.aif.ncsu.edu/ Cell: 267-496-0587
From florjone348nsfp-at-gmail.com Fri Jun 28 14:13:54 2019 Return-Path: {florjone348nsfp-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.162.198] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x5SJDqGe010831 for {microscopylistserverarchive7-at-microscopy.com} ; Fri, 28 Jun 2019 14:13:53 -0500 Received: from mtu23.bigping.com ([Fri, 28 Jun 2019 18:10:17 -0100]) by mmx09.tilkbans.com with SMTP; Fri, 28 Jun 2019 18:10:17 -0100 Message-ID: {0F7DA07A.848BEA34-at-gmail.com}
Hi Karen,
} I'm looking for advice on the use of Adobe Photoshop for image processing } and measuring going forward.
Did you ever consider Fiji (http://fiji.sc/), a version of ImageJ tailored for scientific image analysis?
It is free (and open source), it is extremely powerful, it is platform independent, image analysis is exactly what it is made for ... and there are even dedicated morphometry tools.
Best regards,
Guenter
-- Dr. Guenter Resch Nexperion e.U. - Solutions for Electron Microscopy - www.nexperion.net Ziegelhofstrasse 136/1, 1220 Vienna, Austria - Phone +43 664 94 17 210 Registered at Commercial Court Vienna, FN 397677w - VAT Reg. ATU67962234
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Thanks to all for the quick responses - and on a Friday!
I’m happy to find that so many recommend GIMP or ImageJ/Fiji, both of which I have used a bit in the past, and my colleague Ann Palmer has mentioned (thanks to Chris Winkler, Guenter Resch, James Ehrman, Michael Cammer, Mike Marko and Michael Schoel).
I am leaning toward GIMP because I know the interface can be set up to resemble that of Photoshop. This is an important factor for the multiple analysts in our group who have become accustomed to using Photoshop over the years. However there seem to be more votes for Fiji. Can the Fiji / ImageJ interface be set up with default preferences to resemble Photoshop?
Aperio’s ImageScope is an interesting suggestion (thanks Paula Keene). I have experience using it with the accompanying online Spectrum image database in a histology lab. However I never considered trying it for basic image handling. I just tried opening one of our finished montages in ImageScope but I did not have control over the separate layers (it seemed to flatten the image to a single layer). If this is something that can be easily overcome let me know. Most of my group do have this software already downloaded so having a bit of familiarity with it removes a barrier to acceptance.
Gatan’s Digital Micrograph also seems to be popular (thanks to Roseann Csenscits and Chris Winkler). I didn’t realize there might be a free version for basic image editing and analysis so something to keep in mind. However I think we will pursue Fiji or GIMP first unless there is a compelling reason otherwise.
Not many responses regarding stability of Photoshop itself, although a few seem to be comfortable continuing to use older versions. In our academic healthcare setting we cannot use unsupported software unless we go offline on standalone machines, so that was a concern I forgot to mention.
I don’t want to tie up more bandwidth so if there are further comments, feel free to reply to me privately unless you feel the response would benefit the group.
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Email: tomw-at-uidaho.edu Name: Tom Williams
Organization: Univ of Idaho
Title-Subject: [Filtered] Cleaning Wehnelt cap Message: Greetings,
We are in the middle of troubleshooting gun instability issues on our 2010 TEM and one simple task was to clean hundreds of hours of lanthanum "tarnish" from the the Wehnelt cap. Not so simple! Reasonable success with metal polish on the easy to reach surfaces near the cap aperture but a devil of a time cleaning the INSIDE of the aperture that contains a rather stubborn layer of tarnish. Hesitant to manhandle the cap so I thought I would seek wisdom from the community. Any suggestions for methods or products would be greatly appreciated.
Cheers, Tom
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I can pass along some tricks from FEI and JEOL service engineers. There are a couple of methods to remove the stubborn LaB6 residue from the Wehnelt. One method is to soak the Wehnelt in full strength Micro-90 cleaner overnight, and follow up with a few hours of ultrasonication (using a quality sonicator!) in fresh Micro-90 followed by the metal polish compound. That may not be enough if the Wehnelt hasn't been cleaned in a few filaments, so another method is to scrub the Wehnelt with Bon Ami powder cleanser and a soft toothbrush or other gentle scrubbing tool and again with the metal polish. If you're really struggling, a Dremel with a cotton qtip on low speed coupled with metal polish is a bit more aggressive. You probably will not be able to remove everything no matter what you do, and you should not see any negative effects from a little bit of LaB6 residue around the aperture.
I'm sure others have alternative recipes that will help.
I've been looking at the papers suggested by Robert Keyse and Benjamin Himes. What Spence seems to say is that the contribution to the MIP which is only due to "electrons spilling out a bit further at the surface of a solid" is only around 0.5 V. The main contribution to the MIP is supposed to be due to adsorbed atoms and molecules.
Bill Tivol quite rightly said that then the MIP would not be a property of a specific material, but would depend a lot on what else is around in the vacuum or what the surface came in contact with. Are there any experiments on that?
I've also heard the opinion that surface adsorbates contribute only little to the MIP and that it's mainly due to the solid itself.
Then there's still the question of a thickness independent contribution to the phase shift. A tripple surface charge layer like - + - with balanced charges could explain that, but where would that come from?
All the best,
Philip
X-from: Robert Keyse {rok210-at-lehigh.edu} Sent: Wednesday, 19 June 2019 17:02:11 To: Philip Köck; microscopy-at-microscopy.com Subject: Re: [Microscopy] mean inner potential
Hi Philip,
the Saldin & Spence paper: Ultramicroscopy 55 (1994) 397-406 suggests the surface atoms are less confined and spread their valence electrons out into the vacuum creating a surface dipole that differs from inside the material. . Electrons speed up slightly just as they enter a sample (increase in wave-vector) due to this inner potential.
Reference [12] O'Keefe & Spence: Acta Cryst. (1994) A50, 33-45 (J C H Spence would be a good reference to follow generally) O'Keefe and Spence estimate the surface potential is weak and only about 0.5 volts (page 39: a back of an envelope estimate).
On Tue, Jun 18, 2019 at 3:40 AM {philip.koeck-at-ki.se} wrote:
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Thanks for the reference.
I can't make sense of a thickness-independent contribution to the phase shift either. The way I see it even a surface layer of dipoles would lead to a constant MIP and a phase shift proportional to the thickness.
One can think of a simple model: A slab of completely neutral material (made of neutrons) covered in a layer of positive charge and outside that a layer of negative charge that balances the positive charge. The potential inside this slab will be constant and independent of the thickness of the slab.
I wonder if we can get a comment from someone who knows more.
All the best,
Philip
X-from: 3dem {3dem-bounces-at-ncmir.ucsd.edu} on behalf of Benjamin Himes {himes.benjamin-at-gmail.com} Sent: Monday, 17 June 2019 20:45:48 To: 3dem-at-ncmir.ucsd.edu Subject: [3dem] (mean Inner potential) Re: 3dem Digest, Vol 142, Issue 38
Hi Philip,
The mean inner potential (MIP) refers to a total "interaction" potential that is considered a material property. It consists of all the sources contributing to the potential well seen by an imaging electron, including those you suggest (nuclear and electronic contributions.)
Yes, physical changes to the surface via adsorbed matter will directly affect the MIP. I believe the working hypothesis for the source of the "Volta" potential is through heat/exposure related modification of surface adsorbates.
It is also interesting to note that in addition to the electronic character of the object, the surface contributions of adsorbates and heating, there is another thickness independent phase shift (at least for carbon) the source of which I am not clear on. Happy to hear an explanation from anyone in the know : )
Please have a look at this paper where all of the non-Volta contributions are discussed and also measured.
"Electron holography of thin amorphous carbon films: Measurement of the mean inner potential and a thickness-independent phase shift"
doi: j.ultramic.2005.10.004
HTH
Ben
------------------------ Benjamin Himes
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Some comments: 1. This procedure should be used for stainless parts of Wehnelt only !!! 2. The 10% KOH in ethanol changes the color when the stubborn LaB6 residues are dissolved. 3. The Freon have to be replaced by some other environment friendly solvent. Now we are using isopropyl alcohol.
Best regards
Oldrich
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Not so simple! } Reasonable success with metal polish on the easy to reach surfaces } near the cap aperture but a devil of a time cleaning the INSIDE of } the aperture that contains a rather stubborn layer of tarnish. } Hesitant to manhandle the cap so I thought I would seek wisdom from } the community. 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==============================Original Headers============================== 11, 26 -- From benada-at-biomed.cas.cz Wed Jul 3 04:13:51 2019 11, 26 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) 11, 26 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x639Dolw011513 11, 26 -- for {microscopy-at-microscopy.com} ; Wed, 3 Jul 2019 04:13:50 -0500 11, 26 -- Received: from u117ob02 (nb170ph.mbu.cas.cz [147.231.44.133]) 11, 26 -- (using TLSv1.2 with cipher ECDHE-RSA-AES256-GCM-SHA384 (256/256 bits)) 11, 26 -- (No client certificate requested) 11, 26 -- by mail2.biomed.cas.cz (Postfix) with ESMTPSA id 045BCD00CBC; 11, 26 -- Wed, 3 Jul 2019 11:15:04 +0200 (CEST) 11, 26 -- Date: Wed, 3 Jul 2019 11:15:03 +0200 11, 26 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 11, 26 -- To: {microscopy-at-microscopy.com} , tomw-at-uidaho.edu 11, 26 -- Subject: Re: [Microscopy] viaWWW:Cleaning Wehnelt cap 11, 26 -- Message-ID: {20190703111503.1cef18c3-at-u117ob02} 11, 26 -- In-Reply-To: {201907022356.x62NuQjc024901-at-microscopy.com} 11, 26 -- References: {201907022356.x62NuQjc024901-at-microscopy.com} 11, 26 -- Organization: =?UTF-8?B?TWlrcm9iaW9sb2dpY2vDvSDDunN0YXY=?= AV 11, 26 -- =?UTF-8?B?xIxS?= 11, 26 -- X-Mailer: Claws Mail 3.17.1 (GTK+ 2.24.31; i686-pc-linux-gnu) 11, 26 -- MIME-Version: 1.0 11, 26 -- Content-Type: text/plain; charset=US-ASCII 11, 26 -- Content-Transfer-Encoding: 7bit 11, 26 -- X-IoP-CAS-MailScanner-Information: Please contact the ISP for more information 11, 26 -- X-IoP-CAS-MailScanner-ID: 045BCD00CBC.AD1E0 11, 26 -- X-IoP-CAS-MailScanner: Processed 11, 26 -- X-Spam-Status: No ==============================End of - Headers==============================
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Email: cannonmp-at-comcast.net Name: Bart Cannon
Organization: Cannon Microprobe
Title-Subject: [Filtered] Infrared Spectra From Electron Beam
Message: A 50 nA electron beam will melt and de-nature many materials. Infrared radiation must be produced from the heating. I have never seen mention of collecting infrared spectra during x-ray micro-analysis.
Please direct me to papers about this or any personal experience.
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XEDS detectors are sensitive to any energy deposition, light, heat, mechanical vibrations and x-rays.
For an example of IR radiation which can swamp the low energy end of the spectra have a look at Figure 4 of the following:
Modifications to Membrane Heater Holders to Enable XEDS in the AEM N. J. Zaluzec, A. Janssen, M. G. Burke, D. Gardiner, S. Walden Microsc. Microanal. 21 (Suppl 3), 2015 pg 961-962 doi:10.1017/S1431927615005607
The IR was created by a "heating holder" in the TEM
Cheers,
Nestor Your Friendly Neighborhood SysOp
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Infrared radiation must be } produced from the heating. I have never seen mention of collecting infrared spectra during x-ray } micro-analysis. } } Please direct me to papers about this or any personal experience. } } Login Host: 73.97.160.47 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 10, 53 -- From microscopy.listserver-at-gmail.com Wed Jul 3 07:01:43 2019 } 10, 53 -- Received: from mail-io1-f43.google.com (mail-io1-f43.google.com [209.85.166.43]) } 10, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x63C1hlI025897 } 10, 53 -- for {microscopy-at-microscopy.com} ; Wed, 3 Jul 2019 07:01:43 -0500 } 10, 53 -- Received: by mail-io1-f43.google.com with SMTP id j6so4133885ioa.5 } 10, 53 -- for {microscopy-at-microscopy.com} ; Wed, 03 Jul 2019 05:03:01 -0700 (PDT) } 10, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 10, 53 -- d=gmail.com; s=20161025; } 10, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 10, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 10, 53 -- bh=DFqMB4BohR0jIstuwOc8WDbvqrZRldyNKlHZvKAvZrk=; } 10, 53 -- b=rhhjdv9pyTSFve4Xr7S+K8jair/et5rDH/yRr/mCHK+IhtYWfG/92nFufMDDMxcIln } 10, 53 -- yrcugyIMWvcc82L8oeec30XXnLSJ5NjstoeleCVo4qGz7J3sPbjlDRp5XSiqo9KUcSeK } 10, 53 -- 0mK+uq53gX46GajlSV+EKyX8E3c7I5V2yrGz2NeN/W2rq6JcBiMQt+CLjVjIQ86/Ncza } 10, 53 -- rz6/bqv0arjkn3OY6HjkxUl3V78w/QjOuw4Ps6T+zTJwuIU1skCe+H3P6L48UrGJM4HA } 10, 53 -- JiXF5DGu5dZWeFTwS8pUD8c/PnKVd3MmEPUPIRE5LsHaYU+/36ozvp5wXJS0WCTjjvQK } 10, 53 -- mYVA== } 10, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 10, 53 -- d=1e100.net; s=20161025; } 10, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 10, 53 -- :user-agent:mime-version:in-reply-to:content-language } 10, 53 -- :content-transfer-encoding; } 10, 53 -- bh=DFqMB4BohR0jIstuwOc8WDbvqrZRldyNKlHZvKAvZrk=; } 10, 53 -- b=BIB67PnVvTuGpbmECe9gQbGJVx9WB8QeWGBHvbxidh3z7ME/1L/wTzx9vxP8YGZS/B } 10, 53 -- NsVdNpAgyGGY66SVAaMVwm23fZW4H0lHMC5jUysWhxhi+/KSwPL9TNXMMOb4/AMB1X8W } 10, 53 -- LOt5YEige/y6ayOPYh9W7HslAXnE0a0h5Wpx2aTLQXUjAejhUCMpSxIZ2WweScXSPl/m } 10, 53 -- 2ETN2Vp2rrhCi8dDKI5w/uPSMegP3CUR/TjMpbGSysQxF+W5TtIpulR6fse/5V45EgsE } 10, 53 -- iHnny13D2dLsCerGgUtcPHILQcs6SYZUZiSn1iMMuRBsB2JCoDkJRP7aRgWkowOal65X } 10, 53 -- 6VUg== } 10, 53 -- X-Gm-Message-State: APjAAAVWay3i3l4mVGkRREoPFOjihr62Bh1NjXFCgI/nudFGGJlAjzG3 } 10, 53 -- abEpOzX1SRWmuGbTD45V4vehVH5W } 10, 53 -- X-Google-Smtp-Source: APXvYqwiPz8MHOn9ksvKcNQ96SStBnrvJqtXHTYghxMqvYWeqmZ388Typ69mLD/ndO7/fw6FZA0hYw== } 10, 53 -- X-Received: by 2002:a02:8816:: with SMTP id r22mr42125962jai.60.1562155380490; } 10, 53 -- Wed, 03 Jul 2019 05:03:00 -0700 (PDT) } 10, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:4470:b4ee:8771:3fd2]) } 10, 53 -- by smtp.googlemail.com with ESMTPSA id w23sm3927014ioa.51.2019.07.03.05.02.59 } 10, 53 -- for {microscopy-at-microscopy.com} } 10, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 10, 53 -- Wed, 03 Jul 2019 05:02:59 -0700 (PDT) } 10, 53 -- Subject: [Filtered] viaWWW:Infrared Spectra From Electron Beam } 10, 53 -- References: {201907030933.x639XIrq018967-at-microscopy.com} } 10, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 10, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 10, 53 -- X-Forwarded-Message-Id: {201907030933.x639XIrq018967-at-microscopy.com} } 10, 53 -- Message-ID: {611f2e79-d392-8f9a-9fb6-f877d33b2946-at-gmail.com} } 10, 53 -- Date: Wed, 3 Jul 2019 07:02:58 -0500 } 10, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.14; rv:60.0) } 10, 53 -- Gecko/20100101 Thunderbird/60.7.2 } 10, 53 -- MIME-Version: 1.0 } 10, 53 -- In-Reply-To: {201907030933.x639XIrq018967-at-microscopy.com} } 10, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 10, 53 -- Content-Language: en-US } 10, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== } }
We have a 1000C heating stage on our FEI Quanta. Somewhere upwards of 300C we get enough incandescence that the EDS spectrum is not useful. It happens gradually as the intensity grows. It would be interesting to see what Nestor did to permit XEDS.
I don't suppose the beam heating gets your sample that warm. However, IR spectroscopy might be an interesting exercise. I suppose it has been done but it might be a good time to revisit it with improvements in technology.
Warren Straszheim Materials Analysis and Research Lab Iowa State University
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Wednesday, July 03, 2019 7:31 AM To: Straszheim, Warren E [BIOTC]
Bart
XEDS detectors are sensitive to any energy deposition, light, heat, mechanical vibrations and x-rays.
For an example of IR radiation which can swamp the low energy end of the spectra have a look at Figure 4 of the following:
Modifications to Membrane Heater Holders to Enable XEDS in the AEM N. J. Zaluzec, A. Janssen, M. G. Burke, D. Gardiner, S. Walden Microsc. Microanal. 21 (Suppl 3), 2015 pg 961-962 doi:10.1017/S1431927615005607
The IR was created by a "heating holder" in the TEM
Cheers,
Nestor Your Friendly Neighborhood SysOp
On 7/3/19 7:01 AM, microscopy.listserver-at-gmail.com wrote: } ---------------------------------------------------------------------- } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } X-from: cannonmp-at-comcast.net } } This Question/Comment was submitted to the Microscopy Listserver using } the WWW based Form at http://microscopy.com/MLFormMail.html } ---------------------------------------------------------------------- } ----- Remember this posting is most likely not from a Subscriber, so } when replying please copy to both } cannonmp-at-comcast.net, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can } benefit from our collective wisdom } ---------------------------------------------------------------------- } ----- } } Email: cannonmp-at-comcast.net Name: Bart Cannon } } Organization: Cannon Microprobe } } Title-Subject: [Filtered] Infrared Spectra From Electron Beam } } Message: A 50 nA electron beam will melt and de-nature many materials. } Infrared radiation must be produced from the heating. I have never } seen mention of collecting infrared spectra during x-ray micro-analysis. } } Please direct me to papers about this or any personal experience. } } Login Host: 73.97.160.47 } Listserver Email Form V - 20120416 } ---------------------------------------------------------------------- } ----- } } } } ==============================Original } Headers============================== } 10, 53 -- From microscopy.listserver-at-gmail.com Wed Jul 3 07:01:43 } 2019 10, 53 -- Received: from mail-io1-f43.google.com (mail-io1-f43.google.com [209.85.166.43]) } 10, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x63C1hlI025897 } 10, 53 -- for {microscopy-at-microscopy.com} ; Wed, 3 Jul 2019 07:01:43 -0500 } 10, 53 -- Received: by mail-io1-f43.google.com with SMTP id j6so4133885ioa.5 } 10, 53 -- for {microscopy-at-microscopy.com} ; Wed, 03 Jul 2019 05:03:01 -0700 (PDT) } 10, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 10, 53 -- d=gmail.com; s=20161025; } 10, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 10, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 10, 53 -- bh=DFqMB4BohR0jIstuwOc8WDbvqrZRldyNKlHZvKAvZrk=; } 10, 53 -- b=rhhjdv9pyTSFve4Xr7S+K8jair/et5rDH/yRr/mCHK+IhtYWfG/92nFufMDDMxcIln } 10, 53 -- yrcugyIMWvcc82L8oeec30XXnLSJ5NjstoeleCVo4qGz7J3sPbjlDRp5XSiqo9KUcSeK } 10, 53 -- 0mK+uq53gX46GajlSV+EKyX8E3c7I5V2yrGz2NeN/W2rq6JcBiMQt+CLjVjIQ86/Ncza } 10, 53 -- rz6/bqv0arjkn3OY6HjkxUl3V78w/QjOuw4Ps6T+zTJwuIU1skCe+H3P6L48UrGJM4HA } 10, 53 -- JiXF5DGu5dZWeFTwS8pUD8c/PnKVd3MmEPUPIRE5LsHaYU+/36ozvp5wXJS0WCTjjvQK } 10, 53 -- mYVA== } 10, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 10, 53 -- d=1e100.net; s=20161025; } 10, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 10, 53 -- :user-agent:mime-version:in-reply-to:content-language } 10, 53 -- :content-transfer-encoding; } 10, 53 -- bh=DFqMB4BohR0jIstuwOc8WDbvqrZRldyNKlHZvKAvZrk=; } 10, 53 -- b=BIB67PnVvTuGpbmECe9gQbGJVx9WB8QeWGBHvbxidh3z7ME/1L/wTzx9vxP8YGZS/B } 10, 53 -- NsVdNpAgyGGY66SVAaMVwm23fZW4H0lHMC5jUysWhxhi+/KSwPL9TNXMMOb4/AMB1X8W } 10, 53 -- LOt5YEige/y6ayOPYh9W7HslAXnE0a0h5Wpx2aTLQXUjAejhUCMpSxIZ2WweScXSPl/m } 10, 53 -- 2ETN2Vp2rrhCi8dDKI5w/uPSMegP3CUR/TjMpbGSysQxF+W5TtIpulR6fse/5V45EgsE } 10, 53 -- iHnny13D2dLsCerGgUtcPHILQcs6SYZUZiSn1iMMuRBsB2JCoDkJRP7aRgWkowOal65X } 10, 53 -- 6VUg== } 10, 53 -- X-Gm-Message-State: APjAAAVWay3i3l4mVGkRREoPFOjihr62Bh1NjXFCgI/nudFGGJlAjzG3 } 10, 53 -- abEpOzX1SRWmuGbTD45V4vehVH5W } 10, 53 -- X-Google-Smtp-Source: } APXvYqwiPz8MHOn9ksvKcNQ96SStBnrvJqtXHTYghxMqvYWeqmZ388Typ69mLD/ndO7/fw } 6FZA0hYw== 10, 53 -- X-Received: by 2002:a02:8816:: with SMTP id } r22mr42125962jai.60.1562155380490; } 10, 53 -- Wed, 03 Jul 2019 05:03:00 -0700 (PDT) } 10, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:4470:b4ee:8771:3fd2]) } 10, 53 -- by smtp.googlemail.com with ESMTPSA id w23sm3927014ioa.51.2019.07.03.05.02.59 } 10, 53 -- for {microscopy-at-microscopy.com} } 10, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 10, 53 -- Wed, 03 Jul 2019 05:02:59 -0700 (PDT) } 10, 53 -- Subject: [Filtered] viaWWW:Infrared Spectra From Electron } Beam 10, 53 -- References: } {201907030933.x639XIrq018967-at-microscopy.com} } 10, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 10, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 10, 53 -- X-Forwarded-Message-Id: } {201907030933.x639XIrq018967-at-microscopy.com} } 10, 53 -- Message-ID: {611f2e79-d392-8f9a-9fb6-f877d33b2946-at-gmail.com} } 10, 53 -- Date: Wed, 3 Jul 2019 07:02:58 -0500 10, 53 -- User-Agent: } Mozilla/5.0 (Macintosh; Intel Mac OS X 10.14; rv:60.0) 10, 53 -- } Gecko/20100101 Thunderbird/60.7.2 10, 53 -- MIME-Version: 1.0 10, 53 } -- In-Reply-To: {201907030933.x639XIrq018967-at-microscopy.com} } 10, 53 -- Content-Type: text/plain; charset=windows-1252; } format=flowed 10, 53 -- Content-Language: en-US 10, 53 -- } Content-Transfer-Encoding: 7bit ==============================End of - } Headers============================== } }
Hi Tom, Here is the procedure I use for decades on thermionic e-gun parts. Works beautifully every time with lasting results.
Materials and tools:  - polishing paste such as Wenol or Pol or Pikal  - high density cotton fabric such as cotton lintless cloth  - cotton Q-tips on wooden sticks  - fine grade sandpaper, 1000 or higher  - ultrasonic cleaner  - 3 glass beakers of suitable size  - medium size tweezers  - sharp scissors  - acetone hardware store grade  - dry compress gas (duster can)  - powderless latex gloves  - wearable eye loops or high power reading glasses
Procedure:  - disassemble wehnelt completely to the last screw  - use polishing paste on a cloth to polish open surfaces  - use Q-tips to polish tight spots  - use wooden sticks of Q-tips to polish tightest spots such as apertures  - use sharp-pointed wood sticks (cut with scissors) for reaching inside tightest spots such as aperture  - clean excess of polishing paste with cloth / Q-tips / wood tips  - sonicate in three consecutive acetone baths, 5+ minutes each, do not let them dry between consecutive baths  - when done dry parts in air at room temperature for up to 10 min.  - inspect in bright light wearing high power glasses or eye loops  - assemble and align wearing powderless latex gloves
Comments:  - Wehnelt apertures down to 300um can be cleaned this way (but not obj. / C2 / SA apertures !)  - only parts in "direct view" of cathode tip and "direct view" of emission chamber cavity must have shiny polished metal surface. Wehnelt assembly parts facing each other or porcelain insulator should be "generally clean" yet some minor oxidation is OK  - particles of hard polishing material will slide down while in ultrasonic bath. Position parts so that concave surfaces will face down and convex surfaces will face up as much as possible. If not possible then turn parts of complex shape a few times. This way polishing material will not accumulate inside wehnelt components.  - move parts from one bath to the next with the tweezers, while ultrasonic power is on, and blow excess acetone off with compressed gas before placing part into next bath  - Why acetone? Why hardware store grade? Acetone dries off quickly. One from hardware store, whatever is in it, is 100% VOC and drying with zero residue - all that is required from it for this procedure. And it's cheap.  - sandpaper usually not needed - unless for cleaning of previously neglected parts. If have to use sandpaper just be careful and take it easy, and always follow with polishing paste.
 Good luck!
Vitaly Feingold SIA 2773 Heath Lane Duluth GA 30096 Ph. 770-232-7785 Fax 770-232-1791 www.sia-cam.com vitaly-at-sia-cam.com
On 7/2/2019 7:59 PM, microscopy.listserver-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: tomw-at-uidaho.edu } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy to both } tomw-at-uidaho.edu, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can } benefit from our collective wisdom } --------------------------------------------------------------------------- } } Email: tomw-at-uidaho.edu Name: Tom Williams } } Organization: Univ of Idaho } } Title-Subject: [Filtered] Cleaning Wehnelt cap } Message: Greetings, } } We are in the middle of troubleshooting gun instability issues on our 2010 TEM and one simple task } was to clean hundreds of hours of lanthanum "tarnish" from the the Wehnelt cap. Not so simple! } Reasonable success with metal polish on the easy to reach surfaces near the cap aperture but a devil } of a time cleaning the INSIDE of the aperture that contains a rather stubborn layer of tarnish. } Hesitant to manhandle the cap so I thought I would seek wisdom from the community. Any suggestions } for methods or products would be greatly appreciated. } } Cheers, } Tom } } Login Host: 129.101.58.104 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 10, 53 -- From microscopy.listserver-at-gmail.com Tue Jul 2 18:55:50 2019 } 10, 53 -- Received: from mail-io1-f45.google.com (mail-io1-f45.google.com [209.85.166.45]) } 10, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x62Nto8F023708 } 10, 53 -- for {microscopy-at-microscopy.com} ; Tue, 2 Jul 2019 18:55:50 -0500 } 10, 53 -- Received: by mail-io1-f45.google.com with SMTP id s7so469767iob.11 } 10, 53 -- for {microscopy-at-microscopy.com} ; Tue, 02 Jul 2019 16:57:06 -0700 (PDT) } 10, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 10, 53 -- d=gmail.com; s=20161025; } 10, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 10, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 10, 53 -- bh=+bygn/nx49q1mE334pD7RhqgkkjjsW/CaWclDjPenrY=; } 10, 53 -- b=Mpdnma03dRczHwyIsxgMpAMnecl1+yAHhT5ngearS5jW2qF1wk66T38DqUJhAMoZ9f } 10, 53 -- APOCAxVZoP8vmuGX1DBolxsXLS3GAqxg8yTY10DRexkQaQd8d/ZNoiwSZRihhoJnsTao } 10, 53 -- VrhNEuw3C0UxOALNowHGCV+DmLuFYuDi3U7NP7xHq+4OdSaKdIiR0xgRZkEakFQjTA7T } 10, 53 -- NHADJsjGm9g4lHY/OX5d0LP3IsGj2rszLYEOVQxgM1Qdx81tW39bKWrvmyL3ft2l4oaR } 10, 53 -- HjOl07XYTx4ohJAhEWHe6Lm0rx6WzUfLbb8duYsXnk/lm6mkz1ICiaynaTfma79lQ5w8 } 10, 53 -- 5MoQ== } 10, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 10, 53 -- d=1e100.net; s=20161025; } 10, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 10, 53 -- :user-agent:mime-version:in-reply-to:content-language } 10, 53 -- :content-transfer-encoding; } 10, 53 -- bh=+bygn/nx49q1mE334pD7RhqgkkjjsW/CaWclDjPenrY=; } 10, 53 -- b=QavxDoQMUoaWLqJp6w0ZHrTGVUil3HmRqRWC8SZsnGZwjHHcp8fwbTugKePMfDCjJL } 10, 53 -- D081CZAAsuUMVZM+m6ttbSLAVnRVL1E2qYCv8Z8lIXYhnqzyvpQ6Go4eVrQND4vA/u8G } 10, 53 -- EIohEXuVPcwi6zGSXXvbT0x3z8d2cy9w88cBWITef2mzq6QMRpxS3FODDQdTr62tyXSr } 10, 53 -- AsaNHY/Bq4XyrbutWkTPKHgBK+DpkHI63IUmYN7z83zEme5DsYaRwz7iHqezQYVBekG1 } 10, 53 -- ZqrtKEQ85mwFdUwxV3Cet5szLW7P1ljzfu2uynZJXChqZSpv9raBLRo/fHRWBBfvxRP0 } 10, 53 -- 4ADg== } 10, 53 -- X-Gm-Message-State: APjAAAWag03NHnnlRjdhN3dqN/XJ0A/pb15KJQv4JC3KRGt25QKZQQBJ } 10, 53 -- /KDtGvrNAAEk0PT+ciU8Tpl44NBp } 10, 53 -- X-Google-Smtp-Source: APXvYqwhb/m2ZBWA7MxF6yDPW5y6mviZirwx2qnfvy+TD9UapLZCJ6LM3vLMTv4s+/wbQr9/8p0IYA== } 10, 53 -- X-Received: by 2002:a6b:7606:: with SMTP id g6mr24965427iom.288.1562111826039; } 10, 53 -- Tue, 02 Jul 2019 16:57:06 -0700 (PDT) } 10, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:9caa:b52:588:63fa]) } 10, 53 -- by smtp.googlemail.com with ESMTPSA id w23sm930392ioa.51.2019.07.02.16.57.05 } 10, 53 -- for {microscopy-at-microscopy.com} } 10, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 10, 53 -- Tue, 02 Jul 2019 16:57:05 -0700 (PDT) } 10, 53 -- Subject: viaWWW:Cleaning Wehnelt cap } 10, 53 -- References: {201907021931.x62JVM2D009360-at-microscopy.com} } 10, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 10, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 10, 53 -- X-Forwarded-Message-Id: {201907021931.x62JVM2D009360-at-microscopy.com} } 10, 53 -- Message-ID: {845bd352-b260-4cf6-d29b-436ae33f4041-at-gmail.com} } 10, 53 -- Date: Tue, 2 Jul 2019 18:57:04 -0500 } 10, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.14; rv:60.0) } 10, 53 -- Gecko/20100101 Thunderbird/60.7.2 } 10, 53 -- MIME-Version: 1.0 } 10, 53 -- In-Reply-To: {201907021931.x62JVM2D009360-at-microscopy.com} } 10, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 10, 53 -- Content-Language: en-US } 10, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
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From geracore539rphuy-at-gmail.com Fri Jul 5 22:59:32 2019 Return-Path: {geracore539rphuy-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.162.192] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x663xVKb004232 for {microscopylistserverarchive7-at-microscopy.com} ; Fri, 5 Jul 2019 22:59:32 -0500 Received: from unknown (65.170.93.1) by snmp.otwaloow.com with LOCAL; Fri, 05 Jul 2019 21:47:52 -0600 Message-ID: {0C54B4C4.D1AE287A-at-gmail.com}
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Email: tomw-at-uidaho.edu Name: Thomas Williams
Organization: Univ of Idaho
Title-Subject: [Filtered] Thanks for the High Quality Advice!
Message: Greetings,
Big thanks for all the great advice and info on ridding my wehnelt cap of its unsightly LaB6 grunge. Success on the grunge. Still battling other gremlins but the cap is clean!
Thanks!
Tom
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After a hopeless search on the internet I found myself frustrated with new Slice-and-view program on Helios. I had one on old Strata and we were crafting the recipes outside of the program ( scripting), then saved the file and uploaded it to the FIB. It was very convenient, because saved time for users and ket the instrument usage more efficient. Does anybody have a manual? I just wanted to do my home work before approaching Helios next time.
Thank you in advance! Lolita Rotkina, PhD X-from: Lolita Rotkina {lolita.rotkina-at-yandex.com}
Do any of you have any suggestions for resource scheduling software? I’ve been using my home grown kludge for a while but now that I have to schedule three instruments with users of widely varying proficiencies it would be nice to have a more flexible solution that will let me define rules as to when and how much time a particular user can schedule on an instrument. Free or open source is always nice, but I’m willing to consider commercial as well.
I’ll summarize for the list.
Happy July 4^th from a northern neighbor!
*Glenn Poirier*
*Earth Science / Science de la Terre*
*Research Services* Canadian Museum of Nature Musée canadien de la nature t.613.364.4088
t. (lab) 613.562.5800 x7179
f.613.364.4027
www.nature.ca
{https://nature.ca/}
*Saving the World with Evidence, Knowledge and Inspiration.*/(click to learn more)/ {https://nature.ca/en/about-us/museum-corporation/mission-mandate} *Sauver le monde avec des preuves, des connaissances et de l'inspiration.*/(cliquez pour en savoir plus)/ {https://nature.ca/fr/sujet-musee/mission-organisation/mission-organisation} Planets
I use the FACES system from CCRC in Georgia for years. It is free, you can limit users booking time by ability, hours, frequency, and have multiple instruments. There is even an account option to follow for billing, though I don't use that.
If you have any questions about it, you can PM me.
Regards,
Pat
Research Technician SEM-FIB Facility Office and Lab - C Building C101 Mechanical Engineering Clean Technologies Research Institute Dalhousie University Halifax Nova Scotia B3H 4R2 Canada
902-223-6693
---------------------------------------------------------------------------------------------------- *From:* microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} *Sent:* July 8, 2019 9:37:14 AM *To:* Patricia Scallion *Subject:* [Microscopy] Fwd: Instrument scheduling software
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-------- Forwarded Message --------
X-from: Glenn Poirier {GPoirier-at-nature.ca}
Hi All,
Do any of you have any suggestions for resource scheduling software? I’ve been using my home grown kludge for a while but now that I have to schedule three instruments with users of widely varying proficiencies it would be nice to have a more flexible solution that will let me define rules as to when and how much time a particular user can schedule on an instrument. Free or open source is always nice, but I’m willing to consider commercial as well.
I’ll summarize for the list.
Happy July 4^th from a northern neighbor!
*Glenn Poirier*
*Earth Science / Science de la Terre*
*Research Services* Canadian Museum of Nature Musée canadien de la nature t.613.364.4088
t. (lab) 613.562.5800 x7179
f.613.364.4027
www.nature.ca {http://www.nature.ca}
{https://nature.ca/}
*Saving the World with Evidence, Knowledge and Inspiration.*/(click to learn more)/ {https://nature.ca/en/about-us/museum-corporation/mission-mandate} *Sauver le monde avec des preuves, des connaissances et de l'inspiration.*/(cliquez pour en savoir plus)/ {https://nature.ca/fr/sujet-musee/mission-organisation/mission-organisation} Planets
X-from: Taillon, Joshua A. (Fed) {joshua.taillon-at-nist.gov}
Hi Glenn,
The Center for Nanoscale Science and Technology at NIST developed a software named NEMO that they use internally for reservations, tool status, usage tracking, etc. It is freely available at https://github.com/usnistgov/NEMO, and can be installed using Docker. It may work for your needs.
Good luck, Josh Taillon
-- Any mention of a commercial product or products within this message is for information purposes only. It does not imply any recommendation or endorsement by the National Institute of Standards and Technology (NIST) or the US Government.
From ninabuck952ws-at-gmail.com Tue Jul 9 05:56:46 2019 Return-Path: {ninabuck952ws-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.162.204] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x69AuiDY030958 for {microscopylistserverarchive7-at-microscopy.com} ; Tue, 9 Jul 2019 05:56:45 -0500 Received: from mailout.endmonthnow.com ([125.16.6.216]) by mts.locks.grgtween.net with LOCAL; Tue, 09 Jul 2019 21:46:34 +1100 Received: from unknown (79.22.226.140) by relay-x.misswldrs.com with ASMTP; Tue, 09 Jul 2019 21:41:38 +1100 Received: from [93.23.132.243] by public.micromail.com.au with QMQP; Tue, 09 Jul 2019 21:23:06 +1100 Received: from [128.220.146.31] by m1.gns.snv.thisdomainl.com with NNFMP; Tue, 09 Jul 2019 21:18:21 +1100 Received: from mxs.perenter.com ([Tue, 09 Jul 2019 21:13:09 +1100]) by webmail.halftomorrow.com with ESMTP; Tue, 09 Jul 2019 21:13:09 +1100 Message-ID: {14092691.32DA8495-at-gmail.com}
X-from: Erico Freitas {ericotadeu-at-ufmg.br}
Dear all,
We are having issues with our Chiller ZEM Zephyr ZEM 1000s (GTI Koudetechniek B.V.) and would like to hear from you about that, so we could figure out what's going on.
The machine we use that chiller for is a TEM Tecnai G2-20 (FEI). It's been working for over 10 years and we do exchange water once a year and check the water preassure and temperature every day. It's pretty stable (4.6 bar and 19 +/-1 ÂşC) but now we are struggling with that.
It has not been able to cool the water down below 21 ÂşC, even thought the water mixing valve is set to the minimum temperature. We've seen something on the water reservoir that looked like oil, then we thought about the compressor. We've checked the compressor and it looks normal, the gas preassure is normal (something about 30 psi). We noticed the fan speed on the radiator is lower than the other Zephyr ZEM chiller we have on the other TEM. Then, we changed the fan speed from cut-off mode to slightly above the min speed. The fan speed did increase, but the chiller efficiency did not change at all. There are two things we can thing of, (1) double check the Y-filter on the water hack, and (2) double check the water mixing valve on the chiller.
I will appreciate your oppinion on that.
-- Erico Freitas
Physicist/Microscopist at Center of Microscopy Universidade Federal de Minas Gerais (UFMG) Av. Antônio Carlos, 6627, Campus Pampulha, Belo Horizonte, MG, Brazil. ZIP Code 31270-901. +55-31-3409-7573 +55-31-3409-7575
Coordinator:Transmission Electron Microscopy laboratory
X-from: Helen Turner {helen.turner-at-waikato.ac.nz}
Abstract submission and registration are now open for the *29th New Zealand Conference on Microscopy*. This year to be hosted by the University of Waikato at its Hamilton campus from the *11th - 14th November 2019*.
We have three world renowned speakers, trade show, full social programme, workshops,Trans-Tasman bursary, Keith Williamson medal and Early Career Microscopy Scholarships.
For details please visit the website at: www.ivvy.com.au/event/Y7FLHK/home.html {http://www.ivvy.com.au/event/Y7FLHK/home.html}
Hope to see you there!
-- Helen M Turner  BSc(Hons) Electron Microscope Facility Faculty of Science and Engineering The University of Waikato Private Bag 3105 Hamilton 3240 New Zealand
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Email: wfschneider-at-wisc.edu Name: Bil Schneider
Organization: UW Madison Geosciences
Title-Subject: [Filtered] EPMA Lab Director position at UW MADISON
Message: UW Madison Geosciences is looking for a lab director to oversee operations of two Electron Microprobes and a Scanning Electron Microscope. Full job description and application information can be found at:
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Email: katejermey-at-rms.org.uk Name: Kate Jermey
Organization: Royal Microscopical Society
Title-Subject: [Filtered] Registration Closing Midday for Brand New RMS Event
Message: Don't forget that registration closes today for the Royal Microscopical Society's brand new event....
Single Cell Data Analysis: Current Approaches and Challenges Francis Crick Institute, London, UK Monday 22 July 2019
This meeting will introduce delegates to the concepts of high-dimensional analysis and will help determine what resources need to be provided to the community. As the numbers of parameters measured in biological experiments increases, the need for a robust data analytical approach is vital. In single cell analyses - which will included flow, imaging and mass cytometry, single cell sequencing, and microscopy - this leads to complex datasets. There are a number of analysis approaches, but which is best for your data and why? Make sure you book now to avoid disappointment!
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Email: ravi.thakkar369-at-gmail.com Name: Ravi
Title-Subject: [Filtered] Tecnai User Interface: Multi-function selection options messed up.
Message: Hi, Binding display on Tecnai user interface on Tecnai12 has messed up. Some of the option like R3, L3 written multiple times and fonts are too small to read.
From lacejoly2eepz-at-gmail.com Fri Jul 12 15:07:52 2019 Return-Path: {lacejoly2eepz-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.162.204] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x6CK7pGI010372 for {microscopylistserverarchive7-at-microscopy.com} ; Fri, 12 Jul 2019 15:07:51 -0500 Message-ID: {286FD4EF.99E0BE30-at-gmail.com}
Hello Ravi,
For changing the programmable buttons on the control panel, you can right click anywhere within the GUI legend and add/change what each button does. In your case, if you want to remove a blank assignment, you have to click and hold on the blank spots and drag them out of the GUI legend. They should then disappear and the GUI will reformat automatically. You will need to do this for each tab of the user interface, as the buttons are dynamically linked to those tabs. So be sure to check each tab to make sure things look okay. If you need more help, email me and I can send a video.
Cheers, Chris
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Some } of the option like R3, L3 written multiple times and fonts are too small } to read. } } https://www.flickr.com/photos/97321550-at-N08/48261161766 } } I am trying to delete some repeated option but not able to do it. 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-- Transmission Electron Microscopy Lab Manager Analytical Instrumentation Facility (AIF) NC State University https://www.aif.ncsu.edu/ Cell: 267-496-0587
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Title-Subject: [Filtered] 120 kV TEM recommendations
Message: I'm in the market for a new 120 kV TEM (I currently have a 100 kV FEI Morgagni 268), and trying to decide between the JOEL JEM 1400 or Thermofisher Talos L120C. I would like to hear people's thoughts on reliability and ease of use, thanks! Login Host: 130.85.86.88 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
We use iLab software here in our Core and have 19 different instruments available to our users. The software works on a calendar system, which you can customize for each instrument. The calendars are then linked to a billing system which generates invoices that you can send to individual users and/or their financial managers (whoever pays the bills :) The iLab people are very helpful and will walk you through the set-up and use via teleconferencing. We've tried several systems over the years (including a Microsoft Access databse - UGH!) and have had very little trouble with this system. It's "clean" and efficient. Here's a link that might be helpful: https://www.agilent.com/en/products/lab-management-software.
I fyou have any questions, please feel free to contact me at the e-mail address below. I am the Goddess of the Calendars (also known as an software Admin)
Cheers, Debra M. Townley debrat-at-bcm.edu Integrated Microscopy Core Baylor College of Medicine
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Monday, July 08, 2019 7:31 AM To: Townley, Debra
-------- Forwarded Message --------
X-from: Glenn Poirier {GPoirier-at-nature.ca}
Hi All,
Do any of you have any suggestions for resource scheduling software? I've been using my home grown kludge for a while but now that I have to schedule three instruments with users of widely varying proficiencies it would be nice to have a more flexible solution that will let me define rules as to when and how much time a particular user can schedule on an instrument. Free or open source is always nice, but I'm willing to consider commercial as well.
I'll summarize for the list.
Happy July 4^th from a northern neighbor!
*Glenn Poirier*
*Earth Science / Science de la Terre*
*Research Services* Canadian Museum of Nature Musée canadien de la nature t.613.364.4088
*Saving the World with Evidence, Knowledge and Inspiration.*/(click to learn more)/ {https://urldefense.proofpoint.com/v2/url?u=https-3A__nature.ca_en_about-2Dus_museum-2Dcorporation_mission-2Dmandate&d=DwIB-g&c=ZQs-KZ8oxEw0p81sqgiaRA&r=U9YNsO3V08vtJQJS0T5thw&m=P81ho37fi8em3Gvg0qhkyeun7TbkangsIPOayOEI-70&s=HlZq4icB-j33Bia_2X21yqbDkw9QwRk6Ig2e0a0rYVc&e= } *Sauver le monde avec des preuves, des connaissances et de l'inspiration.*/(cliquez pour en savoir plus)/ {https://urldefense.proofpoint.com/v2/url?u=https-3A__nature.ca_fr_sujet-2Dmusee_mission-2Dorganisation_mission-2Dorganisation&d=DwIB-g&c=ZQs-KZ8oxEw0p81sqgiaRA&r=U9YNsO3V08vtJQJS0T5thw&m=P81ho37fi8em3Gvg0qhkyeun7TbkangsIPOayOEI-70&s=NFUwzqt9eUse5AW4ZOxy_k81LhgammzMNVGoLtd6CUE&e= } Planets
-------- Forwarded Message -------- X-from: Calomeni, Edward {Edward.Calomeni-at-osumc.edu}
Tagide, I have the JOEL 1400, since 2009 and have no problems to speak of in the ten years of ownership. In my opinion, the 120KV scopes on the market are pretty much the same. My concerns when buying one are: 1. Cost and 2. Service.
The cost factor is fairly straight forward. The service is much more critical. I my case, I run a clinical diagnostic EM facility and I cannot be 'down' for longer than a day. My service tech lives within an hour and thus is very convenient. The other two scope services reps were at least three hours away. Another consideration is the cost of a service contract. Find out what these are before you purchase a scope, this could be a long term savings/cost.
If you can, go somewhere and play with each of them to see if they fit your type of work.
Ed
Edward P. Calomeni Director EM Lab - Pathology The Ohio State University M018 Starling Loving Hall 320 W. 10th Ave. Columbus, OH 43210 614-293-5580 (office) 614-293-8806 (lab) edward.calomeni-at-osumc.edu
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Monday, July 15, 2019 6:57 PM To: Calomeni, Edward
X-from: tagided-at-umbc.edu
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Title-Subject: [Filtered] 120 kV TEM recommendations
Message: I'm in the market for a new 120 kV TEM (I currently have a 100 kV FEI Morgagni 268), and trying to decide between the JOEL JEM 1400 or Thermofisher Talos L120C. I would like to hear people's thoughts on reliability and ease of use, thanks! Login Host: https://urldefense.proofpoint.com/v2/url?u=http-3A__130.85.86.88&d=DwIBAg&c=k9MF1d71ITtkuJx-PdWme51dKbmfPEvxwt8SFEkBfs4&r=y_s11-mGsfinDkHa-3tmefE7s37ShEC3FUqfTsvj2Ig&m=08v9ImgTZOCvQb5ldqFNInmYwEvTMDnyZ169dp73vNI&s=UA-9iawrQPLTAs06PpQd6DeiQ9KT6HmNx3NygmfJICI&e= Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
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-------- Forwarded Message -------- X-from: Richard Van Camp {rvancamp-at-kettering.edu}
Hello Erico,
Does the refrigerant handling system on your Zephyr feature a sight glass? I suspect yet but, do not know. If you see bubbles passing by it it will need additional refrigerant. The presence of bubbles in your refrigerant does not necessarily represent an problem but, it can if your microscope load is sufficiently high. I experienced this problem last spring but, it did not manifest itself as a problem for us.
We own a Haskris that features a remote mounted condenser coil on the roof of the building. This remote mounting location requires that the condenser coils are checked 1x or 2x/year for accumulated debris such as that given off by Dogwood and Cottonwood trees.
Good luck,
Rick
On Fri, Jul 12, 2019 at 3:20 PM {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote:
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X-from:Â Â Â Â Â Erico Freitas {ericotadeu-at-ufmg.br {mailto:ericotadeu-at-ufmg.br} }
Dear all,
We are having issues with our Chiller ZEM Zephyr ZEM 1000s (GTI Koudetechniek B.V.) and would like to hear from you about that, so we could figure out what's going on.
The machine we use that chiller for is a TEM Tecnai G2-20 (FEI). It's been working for over 10 years and we do exchange water once a year and check the water preassure and temperature every day. It's pretty stable (4.6 bar and 19 +/-1 ÂşC) but now we are struggling with that.
It has not been able to cool the water down below 21 ÂşC, even thought the water mixing valve is set to the minimum temperature. We've seen something on the water reservoir that looked like oil, then we thought about the compressor. We've checked the compressor and it looks normal, the gas preassure is normal (something about 30 psi). We noticed the fan speed on the radiator is lower than the other Zephyr ZEM chiller we have on the other TEM. Then, we changed the fan speed from cut-off mode to slightly above the min speed. The fan speed did increase, but the chiller efficiency did not change at all. There are two things we can thing of, (1) double check the Y-filter on the water hack, and (2) double check the water mixing valve on the chiller.
I will appreciate your oppinion on that.
-- Erico Freitas
Physicist/Microscopist at Center of Microscopy Universidade Federal de Minas Gerais (UFMG) Av. Antônio Carlos, 6627, Campus Pampulha, Belo Horizonte, MG, Brazil. ZIP Code 31270-901. +55-31-3409-7573 +55-31-3409-7575
Coordinator:Transmission Electron Microscopy laboratory
==============================Original Headers============================== 11, 53 -- From microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} Fri Jul 12 13:04:45 2019 11, 53 -- Received: from mail-wr1-f44.google.com {http://mail-wr1-f44.google.com} (mail-wr1-f44.google.com {http://mail-wr1-f44.google.com} [209.85.221.44]) 11, 53 --Â Â Â Â by microscopy.com {http://microscopy.com} (8.12.11.20060308/8.12.8) with ESMTP id x6CI4iGu005906 11, 53 --Â Â Â Â for {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } ; Fri, 12 Jul 2019 13:04:44 -0500 11, 53 -- Received: by mail-wr1-f44.google.com {http://mail-wr1-f44.google.com} with SMTP id r1so10829898wrl.7 11, 53 --Â Â Â Â Â for {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } ; Fri, 12 Jul 2019 11:06:32 -0700 (PDT) 11, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 11, 53 --Â Â Â Â Â d=gmail.com {http://gmail.com} ; s=20161025; 11, 53 --Â Â Â Â Â h=subject:references:to:from:message-id:date:user-agent:mime-version 11, 53 --Â Â Â Â Â :in-reply-to:content-language:content-transfer-encoding; 11, 53 --Â Â Â Â Â bh=oB2ve7T7anhFEZPTiGBR8kkHqzrXDgUXE+2YjJE7e5w=; 11, 53 --Â Â Â Â Â b=ABp4buhFLs0wfUQvqqg62zQyYZe6jKTDEeqDevcY7PNyT2viUJCef6nv2pyATgiIQu 11, 53 --Â Â Â Â Â NYwwIOHOBvDSFuISmmkanQ572hODbMfS5xnTFLCnepemo9Oyatqp2acJCnEfqRgkGmMu 11, 53 --Â Â Â Â Â CdueJrwdio86xUqMdHQREUj03uDLC5/MH6TwYjvuGBnbEI0e8jM2F9EFI/6lJAcJbcrj 11, 53 --Â Â Â Â Â E180+I+YrkmlaethxF85vkNlFvwkS8zldNWPj11q8T2bv7WJnPX91LMbSu6a42WEozvA 11, 53 --Â Â Â Â Â j4qZm4HFR8UuJm0OeqyRyhtRrIL33x/l7A7K8g6f20oCZcCIcAyAF6a/22ORm6tWfot6 11, 53 --Â Â Â Â Â PtIg== 11, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 11, 53 --Â Â Â Â Â d=1e100.net {http://1e100.net} ; s=20161025; 11, 53 --Â Â Â Â Â h=x-gm-message-state:subject:references:to:from:message-id:date 11, 53 --Â Â Â Â Â :user-agent:mime-version:in-reply-to:content-language 11, 53 --Â Â Â Â Â :content-transfer-encoding; 11, 53 --Â Â Â Â Â bh=oB2ve7T7anhFEZPTiGBR8kkHqzrXDgUXE+2YjJE7e5w=; 11, 53 --Â Â Â Â Â b=EefP8hVbwZu7I0Oq+SS2+Q2sgFWmqexm7FyA8fJbFv2AvUX6vhzxtpIAFob/4BcRw1 11, 53 --Â Â Â Â Â AhnoenVu5YrfylUZVgjZysxIxMaaKe5FBNAZQmoDa0ENw1R236zWChdN40yQYK1OQidL 11, 53 --Â Â Â Â Â WufI/T1POyquiKZCrwm2VC/lhBuVr3fRGN5a8yxyJXtmn3jJKe97qgiDePBtczSxD/+E 11, 53 --Â Â Â Â Â 59PJJxmz0P9YA9AaFJdsIsYoasK12ABm0tCZ73Dnxc9Y7ZMToG2WxQsJwP6aC8f6S0yN 11, 53 --Â Â Â Â Â 3SeT3EbqaAzbiEDmIuqwbWa93iLu3cXfQNEFOxi9bEo+ahgP7WN5MYW8IVj4eBkZNukE 11, 53 --Â Â Â Â Â U+OQ== 11, 53 -- X-Gm-Message-State: APjAAAX6Y7x8xjXQoufkIPfzGeVQNuA35GikJW9FnoqreyfAW+t+HYzB 11, 53 --Â Â Â Â USQq2s0ryNaVmT9JddlSQbpDwrzikBfa9g== 11, 53 -- X-Google-Smtp-Source: APXvYqzsXQnrI8737YtYSLxTukVVR5hxBcS0gdGUemzO/lJref+3zOL383qL7ErINe/WZO46VccYJA== 11, 53 -- X-Received: by 2002:adf:dd03:: with SMTP id a3mr13582731wrm.87.1562954791781; 11, 53 --Â Â Â Â Â Fri, 12 Jul 2019 11:06:31 -0700 (PDT) 11, 53 -- Received: from Nestor-MacBookAir-Pro-2014-ElCapitan.local ([213.99.16.34]) 11, 53 --Â Â Â Â Â by smtp.googlemail.com {http://smtp.googlemail.com} with ESMTPSA id a64sm12340221wmf.1.2019.07.12.11.06.30 11, 53 --Â Â Â Â Â for {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } 11, 53 --Â Â Â Â Â (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 11, 53 --Â Â Â Â Â Fri, 12 Jul 2019 11:06:31 -0700 (PDT) 11, 53 -- Subject: Fwd: Chiller ZEM Zephyr 1000S - Issue - (TEM Tecnai G2-20 FEI) 11, 53 -- References: {CA+jsbz_ztJ_iQabvuFyvO=mNzjJ3USdsrC3AzJLZwVcmJ1PQ5Q-at-mail.gmail.com {mailto:mNzjJ3USdsrC3AzJLZwVcmJ1PQ5Q-at-mail.gmail.com} } 11, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } 11, 53 -- From: Microscopy Listserver {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } 11, 53 -- X-Forwarded-Message-Id: {CA+jsbz_ztJ_iQabvuFyvO=mNzjJ3USdsrC3AzJLZwVcmJ1PQ5Q-at-mail.gmail.com {mailto:mNzjJ3USdsrC3AzJLZwVcmJ1PQ5Q-at-mail.gmail.com} } 11, 53 -- Message-ID: {bac8bb6e-a27e-0719-1ca5-b633fb61dcdd-at-gmail.com {mailto:bac8bb6e-a27e-0719-1ca5-b633fb61dcdd-at-gmail.com} } 11, 53 -- Date: Fri, 12 Jul 2019 20:06:11 +0200 11, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.13; rv:60.0) 11, 53 --Â Gecko/20100101 Thunderbird/60.7.2 11, 53 -- MIME-Version: 1.0 11, 53 -- In-Reply-To: {CA+jsbz_ztJ_iQabvuFyvO=mNzjJ3USdsrC3AzJLZwVcmJ1PQ5Q-at-mail.gmail.com {mailto:mNzjJ3USdsrC3AzJLZwVcmJ1PQ5Q-at-mail.gmail.com} } 11, 53 -- Content-Type: text/plain; charset=utf-8; format=flowed 11, 53 -- Content-Language: en-US 11, 53 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
-- Instrumentation Specialist Kettering University Department of Physics 1700 University Ave. 1-903 Academic Building Flint, MI 48504 (810) 762-9896
-------- Forwarded Message -------- X-from: Gilpin, Christopher J {gilpin-at-purdue.edu} We also have iLab. In fact we have a site license and have around 80 separate cores using it - including EM, light microscopy etc
​​​​​ Chris
Christopher J. Gilpin Ph.D. Campus-wide Coordinator for Electron Microscopy Director, Life Science Microscopy Facility Purdue University Whistler Hall of Agriculture Research, Room S052 170 S. University St West Lafayette, IN 47907 765-494-7750 gilpin-at-purdue.edu lsmf-at-purdue.edu reaches everyone in the facility. http://ag.purdue.edu/arp/Microscopy/Pages/default.aspx skype cjgilpin
-----Original Message----- X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Monday, July 15, 2019 7:03 PM To: Gilpin, Christopher J {gilpin-at-purdue.edu}
-------- Forwarded Message --------
X-from: Townley, Debra {debrat-at-bcm.edu}
Hi Glenn,
We use iLab software here in our Core and have 19 different instruments available to our users. The software works on a calendar system, which you can customize for each instrument. The calendars are then linked to a billing system which generates invoices that you can send to individual users and/or their financial managers (whoever pays the bills :) The iLab people are very helpful and will walk you through the set-up and use via teleconferencing. We've tried several systems over the years (including a Microsoft Access databse - UGH!) and have had very little trouble with this system. It's "clean" and efficient. Here's a link that might be helpful: https://www.agilent.com/en/products/lab-management-software.
I fyou have any questions, please feel free to contact me at the e-mail address below. I am the Goddess of the Calendars (also known as an software Admin)
Cheers, Debra M. Townley debrat-at-bcm.edu Integrated Microscopy Core Baylor College of Medicine
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Monday, July 08, 2019 7:31 AM To: Townley, Debra
-------- Forwarded Message --------
X-from: Glenn Poirier {GPoirier-at-nature.ca}
Hi All,
Do any of you have any suggestions for resource scheduling software? I've been using my home grown kludge for a while but now that I have to schedule three instruments with users of widely varying proficiencies it would be nice to have a more flexible solution that will let me define rules as to when  and how much time a particular user can schedule on an instrument. Free or open source is always nice, but I'm willing to consider commercial as well.
*Saving the World with Evidence, Knowledge and Inspiration.*/(click to learn more)/ {https://urldefense.proofpoint.com/v2/url?u=https-3A__nature.ca_en_about-2Dus_museum-2Dcorporation_mission-2Dmandate&d=DwIB-g&c=ZQs-KZ8oxEw0p81sqgiaRA&r=U9YNsO3V08vtJQJS0T5thw&m=P81ho37fi8em3Gvg0qhkyeun7TbkangsIPOayOEI-70&s=HlZq4icB-j33Bia_2X21yqbDkw9QwRk6Ig2e0a0rYVc&e= } *Sauver le monde avec des preuves, des connaissances et de l'inspiration.*/(cliquez pour en savoir plus)/ {https://urldefense.proofpoint.com/v2/url?u=https-3A__nature.ca_fr_sujet-2Dmusee_mission-2Dorganisation_mission-2Dorganisation&d=DwIB-g&c=ZQs-KZ8oxEw0p81sqgiaRA&r=U9YNsO3V08vtJQJS0T5thw&m=P81ho37fi8em3Gvg0qhkyeun7TbkangsIPOayOEI-70&s=NFUwzqt9eUse5AW4ZOxy_k81LhgammzMNVGoLtd6CUE&e= } Planets
Hi We just bought a used Jeol JSM5600LV that starts up normally but fails to achieve operative vacuum. I’ve checked hoses + stage in the first place and everything looks fine. Before checking other more difficult points I would like to ask if anybody has an idea regarding possible sources of vacuum leak. By the way, the column of the instrument looks like it has been disassembled and reassembled for some reason.
Hi We just bought a used Jeol JSM5600LV that starts up normally but fails to achieve operative vacuum. Diffusion pump starts but the accessory Pirani indicates vacuum ca 10 times less than the normal one. I’ve checked hoses + stage in the first place and everything looks fine. Before checking other more difficult points I would like to ask if anybody has an idea regarding possible sources of vacuum leak. By the way, the column of the instrument looks like it has been disassembled and reassembled for some reason. Best Regards yorgos Dr Yorgos Nikas Athens Innovative Microscopy Skra 36 Voula 16673 GREECE
Hello Yorgos, I would suggest to check the Pirani gauge. If it is contaminated then its reading might be incorrect (lesser then real value).
Regards
Oldrich
-- OldĹ™ich Benada Institute of Microbiology, Czech Acad. Sci. Laboratory of Molecular Structure Characterization Electron Microscopy Group VĂdeĹská 1083 142 20 Prague 4 Czech Republic
On Wed, 17 Jul 2019 05:47:31 -0500, eikonika-at-otenet.gr wrote : } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi } We just bought a used Jeol JSM5600LV that starts up normally but } fails to achieve operative vacuum. Diffusion pump starts but the } accessory Pirani indicates vacuum ca 10 times less than the normal } one. I_ve checked hoses + stage in the first place and everything } looks fine. Before checking other more difficult points I would like } to ask if anybody has an idea regarding possible sources of vacuum } leak. By the way, the column of the instrument looks like it has been } disassembled and reassembled for some reason. } Best Regards } yorgos } Dr Yorgos Nikas } Athens_ Innovative_ Microscopy } Skra 36 Voula 16673 GREECE } _ } Tel/fax +30 210 8957677 } mobile +30 6945 107477 } www.eikonika.net_www.aim.cat } ************************************* } } } } } ==============================Original } Headers============================== 4, 21 -- From } eikonika-at-otenet.gr Wed Jul 17 05:47:04 2019 4, 21 -- Received: from } chimaera.otenet.gr (smtp-out32.otenet.gr [83.235.69.32]) 4, 21 -- } by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } x6HAl4KP008847 4, 21 -- for {microscopy-at-microscopy.com} ; Wed, } 17 Jul 2019 05:47:04 -0500 4, 21 -- Received: from ozymandias } (ppp-2-84-171-217.home.otenet.gr [2.84.171.217]) 4, 21 -- } (Authenticated sender: eikonika-at-otenet.gr) 4, 21 -- by } chimaera.otenet.gr (ESMTP) with ESMTPSA 4, 21 -- for } {microscopy-at-microscopy.com} ; Wed, 17 Jul 2019 13:49:05 +0300 (EEST) } 4, 21 -- From: "Yorgos Nikas" {eikonika-at-otenet.gr} 4, 21 -- To: } {microscopy-at-microscopy.com} 4, 21 -- Subject: JSM 5600LV vacuum leak } -updated 4, 21 -- Date: Wed, 17 Jul 2019 13:49:00 +0300 4, 21 -- } Message-ID: {000301d53c8d$3e013720$ba03a560$-at-otenet.gr} 4, 21 -- } MIME-Version: 1.0 4, 21 -- Content-Type: text/plain; } 4, 21 -- charset="iso-8859-1" } 4, 21 -- X-Mailer: Microsoft Outlook 14.0 } 4, 21 -- Thread-Index: AdU8jRj5xRpMEWcUSVaRhY1ZNehfLw== } 4, 21 -- Content-Language: en-us } 4, 21 -- Content-Transfer-Encoding: 8bit } 4, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } microscopy.com id x6HAl4KP008847 ==============================End of } - Headers==============================
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==============================Original Headers============================== 10, 27 -- From benada-at-biomed.cas.cz Wed Jul 17 06:26:25 2019 10, 27 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) 10, 27 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x6HBQO5Q020844 10, 27 -- for {microscopy-at-microscopy.com} ; Wed, 17 Jul 2019 06:26:25 -0500 10, 27 -- Received: from u117ob02 (nb170ph.mbu.cas.cz [147.231.44.133]) 10, 27 -- (using TLSv1.2 with cipher ECDHE-RSA-AES256-GCM-SHA384 (256/256 bits)) 10, 27 -- (No client certificate requested) 10, 27 -- by mail2.biomed.cas.cz (Postfix) with ESMTPSA id D8E7BD01052; 10, 27 -- Wed, 17 Jul 2019 13:28:23 +0200 (CEST) 10, 27 -- Date: Wed, 17 Jul 2019 13:28:23 +0200 10, 27 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 10, 27 -- To: eikonika-at-otenet.gr, {microscopy-at-microscopy.com} 10, 27 -- Subject: Re: [Microscopy] JSM 5600LV vacuum leak -updated 10, 27 -- Message-ID: {20190717132823.62def484-at-u117ob02} 10, 27 -- In-Reply-To: {201907171047.x6HAlV6D009289-at-microscopy.com} 10, 27 -- References: {201907171047.x6HAlV6D009289-at-microscopy.com} 10, 27 -- Organization: =?UTF-8?B?TWlrcm9iaW9sb2dpY2vDvSDDunN0YXY=?= AV 10, 27 -- =?UTF-8?B?xIxS?= 10, 27 -- X-Mailer: Claws Mail 3.17.3 (GTK+ 2.24.32; i686-pc-linux-gnu) 10, 27 -- MIME-Version: 1.0 10, 27 -- Content-Type: text/plain; charset=UTF-8 10, 27 -- X-IoP-CAS-MailScanner-Information: Please contact the ISP for more information 10, 27 -- X-IoP-CAS-MailScanner-ID: D8E7BD01052.A9CB1 10, 27 -- X-IoP-CAS-MailScanner: Processed 10, 27 -- X-Spam-Status: No 10, 27 -- Content-Transfer-Encoding: 8bit 10, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id x6HBQO5Q020844 ==============================End of - Headers==============================
From briafloy9huo-at-gmail.com Wed Jul 17 10:15:00 2019 Return-Path: {briafloy9huo-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.162.202] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x6HFExlP005970 for {microscopylistserverarchive7-at-microscopy.com} ; Wed, 17 Jul 2019 10:15:00 -0500 Message-ID: {3DB824E6.BAAC7BB5-at-gmail.com}
I would like to thank you all that have replied the message. I appreciated that.
The issue on our Zephyr chiller is partially solved. We added refrigerant on the compressor and set the its pressure to 40 psi, as it is the pressure on the other chiller we have on other TEM. And it worked again. We also cleaned the filter and replaced the water several times from Friday to Last Monday to have the system cleaned, and put destilled water afterwards and added ThermoClean DC. We're now on the track of the likely leak on the system.
Thanks again.
Cheers,
On 16 Jul 2019 5:31 p.m., {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote:
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-------- Forwarded Message -------- X-from:Â Â Â Â Â Richard Van Camp {rvancamp-at-kettering.edu {mailto:rvancamp-at-kettering.edu} }
Hello Erico,
Does the refrigerant handling system on your Zephyr feature a sight glass? I suspect yet but, do not know. If you see bubbles passing by it it will need additional refrigerant. The presence of bubbles in your refrigerant does not necessarily represent an problem but, it can if your microscope load is sufficiently high. I experienced this problem last spring but, it did not manifest itself as a problem for us.
We own a Haskris that features a remote mounted condenser coil on the roof of the building. This remote mounting location requires that the condenser coils are checked 1x or 2x/year for accumulated debris such as that given off by Dogwood and Cottonwood trees.
Good luck,
Rick
On Fri, Jul 12, 2019 at 3:20 PM {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} {mailto:microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } } wrote:
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   X-from:     Erico Freitas {ericotadeu-at-ufmg.br {mailto:ericotadeu-at-ufmg.br} {mailto:ericotadeu-at-ufmg.br {mailto:ericotadeu-at-ufmg.br} } }
   Dear all,
   We are having issues with our Chiller ZEM Zephyr ZEM 1000s (GTI    Koudetechniek B.V.) and would like to hear from you about that, so we    could figure out what's going on.
   The machine we use that chiller for is a TEM Tecnai G2-20 (FEI). It's    been working for over 10 years and we do exchange water once a year and    check the water preassure and temperature every day. It's pretty stable    (4.6 bar and 19 +/-1 ºC) but now we are struggling with that.
   It has not been able to cool the water down below 21 ºC, even thought    the water mixing valve is set to the minimum temperature.    We've seen something on the water reservoir that looked like oil, then    we thought about the compressor. We've checked the compressor and it    looks normal, the gas preassure is normal (something about 30 psi).    We noticed the fan speed on the radiator is lower than the other Zephyr    ZEM chiller we have on the other TEM. Then, we changed the fan speed    from cut-off mode to slightly above the min speed. The fan speed did    increase, but the chiller efficiency did not change at all.    There are two things we can thing of, (1) double check the Y-filter on    the water hack, and (2) double check the water mixing valve on the chiller.
   I will appreciate your oppinion on that.
   --   Erico Freitas
   Physicist/Microscopist at Center of Microscopy    Universidade Federal de Minas Gerais (UFMG)    Av. Antônio Carlos, 6627, Campus Pampulha, Belo Horizonte, MG, Brazil.    ZIP Code 31270-901.    +55-31-3409-7573    +55-31-3409-7575
   Coordinator:Transmission Electron Microscopy laboratory
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-- Instrumentation Specialist Kettering University Department of Physics 1700 University Ave. 1-903 Academic Building Flint, MI 48504 (810) 762-9896
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-------- Forwarded Message -------- X-from: Frank Karl {frank_karl-at-ardl.com}
I'm hoping the microscopy community can help win a bet for someone. What support equipment do you need to run cleanliness testing for the pharmacology industry and hydraulic fluid industries? Years ago I worked in the medical device area and we did all our cleaning and collection in a positive pressure class 100 hood and the manual counting in a positive pressure class 1000 room. We used .22 micron filtered water, isopropanol and Freon to clean, wash and flush medical devices.
We didn't need bunny suits, except in the manufacturing area. Is this the same type of support equipment used 30 years later or is it more stringent? Is there a A2lA standard, or ASTM standard about this?
Stay safe...........
Frank Karl Microscopist Akron Rubber Development Laboratory 2887 Gilchrist Road Akron, Ohio 44305
Yes, Christopher, we have ~27 Cores using iLab. I like it because we are not a very "hypodermic" lab (research in = results out), but rather prefer to teach our users how to operate microscopes, stain slides, analyze data, etc. We will also provide technical assistance and have a complete TEM processing-to-image facility. iLab can be customized for the consumer's each and every need, which I like very much.
Cheers,
Debra M Townley
Integrated Microscopy Core
Baylor College of Medicine
-------- Forwarded Message -------- X-from: Gilpin, Christopher J {gilpin-at-purdue.edu}
We also have iLab. In fact we have a site license and have around 80 separate cores using it - including EM, light microscopy etc
​​​​​ Chris
Christopher J. Gilpin Ph.D. Campus-wide Coordinator for Electron Microscopy Director, Life Science Microscopy Facility Purdue University Whistler Hall of Agriculture Research, Room S052 170 S. University St West Lafayette, IN 47907 765-494-7750 gilpin-at-purdue.edu lsmf-at-purdue.edu reaches everyone in the facility. https://urldefense.proofpoint.com/v2/url?u=http-3A__ag.purdue.edu_arp_Microscopy_Pages_default.aspx&d=DwIBaQ&c=ZQs-KZ8oxEw0p81sqgiaRA&r=U9YNsO3V08vtJQJS0T5thw&m=essJpLf-LZOxpCDYPQ5xkbA5mtACc6NITYMHFqc3HE4&s=uzvIvxQptEf20bDL9IXPJgfyHzsFSiSqFSNh-zCuPmo&e=
skype cjgilpin
-----Original Message----- X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Monday, July 15, 2019 7:03 PM To: Gilpin, Christopher J {gilpin-at-purdue.edu}
On-Line Help https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.com_MicroscopyListserver_FAQ.html&d=DwIB-g&c=ZQs-KZ8oxEw0p81sqgiaRA&r=U9YNsO3V08vtJQJS0T5thw&m=P81ho37fi8em3Gvg0qhkyeun7TbkangsIPOayOEI-70&s=h4Hj3hKLiOtX4MLSV5_amQdCQ43pKuQWytiXqjy7ax8&e=
Do any of you have any suggestions for resource scheduling software? I've been using my home grown kludge for a while but now that I have to schedule three instruments with users of widely varying proficiencies it would be nice to have a more flexible solution that will let me define rules as to when  and how much time a particular user can schedule on an instrument. Free or open source is always nice, but I'm willing to consider commercial as well.
*Saving the World with Evidence, Knowledge and Inspiration.*/(click to learn more)/ {https://urldefense.proofpoint.com/v2/url?u=https-3A__nature.ca_en_about-2Dus_museum-2Dcorporation_mission-2Dmandate&d=DwIB-g&c=ZQs-KZ8oxEw0p81sqgiaRA&r=U9YNsO3V08vtJQJS0T5thw&m=P81ho37fi8em3Gvg0qhkyeun7TbkangsIPOayOEI-70&s=HlZq4icB-j33Bia_2X21yqbDkw9QwRk6Ig2e0a0rYVc&e=
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We have two of these chillers running since 2004. Both have had problems similar to what you describe with a degraded ability to cool and also the oily residue on the surface of the water in the tank.
When the cooling problem has happened it is usually the three-way mixing valve with the thermostat attached. These valves are a bit of an odd shape and difficult to get (not too different from a simple radiator valve).
The valve can be removed and disassembled by a mechanical workshop and cleaned out. Normally it is a sticking problem. We were able to purchase one from FEI last time. However, the cost was enough that I have the old one sitting in my lab ready for cleaning, should we need one quickly (one of the downsides of living in New Zealand is that it can take many weeks for uncommon parts to arrive).
The material on the surface of the water in the tank in our case was not oil, but appeared to be a residue that we think is from the breakdown of plastic in the system over time and is also copper rich. The layer on the surface we had was very thin and took on an oil-like iridescence through thin-film interreference. It has been happening for about 6 years and not caused a problem that we have observed.
You may want to collect some of the material and investigate to check that it is not oil.
Hope that helps.
Kind regards Duane
-----Original Message----- X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Saturday, 13 July 2019 6:17 AM To: Harland, Duane {Duane.Harland-at-agresearch.co.nz}
X-from: Erico Freitas {ericotadeu-at-ufmg.br}
Dear all,
We are having issues with our Chiller ZEM Zephyr ZEM 1000s (GTI Koudetechniek B.V.) and would like to hear from you about that, so we could figure out what's going on.
The machine we use that chiller for is a TEM Tecnai G2-20 (FEI). It's been working for over 10 years and we do exchange water once a year and check the water preassure and temperature every day. It's pretty stable (4.6 bar and 19 +/-1 ÂşC) but now we are struggling with that.
It has not been able to cool the water down below 21 ÂşC, even thought the water mixing valve is set to the minimum temperature. We've seen something on the water reservoir that looked like oil, then we thought about the compressor. We've checked the compressor and it looks normal, the gas preassure is normal (something about 30 psi). We noticed the fan speed on the radiator is lower than the other Zephyr ZEM chiller we have on the other TEM. Then, we changed the fan speed from cut-off mode to slightly above the min speed. The fan speed did increase, but the chiller efficiency did not change at all. There are two things we can thing of, (1) double check the Y-filter on the water hack, and (2) double check the water mixing valve on the chiller.
I will appreciate your oppinion on that.
-- Erico Freitas
Physicist/Microscopist at Center of Microscopy Universidade Federal de Minas Gerais (UFMG) Av. AntĂ´nio Carlos, 6627, Campus Pampulha, Belo Horizonte, MG, Brazil. ZIP Code 31270-901. +55-31-3409-7573 +55-31-3409-7575
Coordinator:Transmission Electron Microscopy laboratory
Hi Many thanks to everybody who replied to my message. Using an external Pirani we checked the vacuum values and validate them against a second -working- JSM5600LV we have. Both RPs (rotary pumps-high and low vacuum) work well and Diffusion pump starts at the right time. When pre-evacuation starts, you can tell that the vacuum force applied to the stage is weak and the noise of the RP is like working on open air for many seconds. And also, a few moments after I switch off the machine there is no more vacuum in the hose connecting the RP (HV) with the microscope, while there is in the chamber . We think that we have a real vacuum problem and suspect the valves lying below the column but we have no knowledge how to check them. Thanks again
Hi We just bought a used Jeol JSM5600LV that starts up normally but fails to achieve operative vacuum. Diffusion pump starts but the accessory Pirani indicates vacuum ca 10 times less than the normal one. I’ve checked hoses + stage in the first place and everything looks fine. Before checking other more difficult points I would like to ask if anybody has an idea regarding possible sources of vacuum leak. By the way, the column of the instrument looks like it has been disassembled and reassembled for some reason. Best Regards yorgos Dr Yorgos Nikas Athens Innovative Microscopy Skra 36 Voula 16673 GREECE
==============================Original Headers============================== 5, 23 -- From eikonika-at-otenet.gr Sat Jul 20 00:32:41 2019 5, 23 -- Received: from medusa.otenet.gr (smtp-out31.otenet.gr [83.235.69.31]) 5, 23 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x6K5Weg7021341 5, 23 -- for {microscopy-at-microscopy.com} ; Sat, 20 Jul 2019 00:32:41 -0500 5, 23 -- Received: from VAIO (unknown [100.76.221.19]) 5, 23 -- (Authenticated sender: eikonika-at-otenet.gr) 5, 23 -- by medusa.otenet.gr (ESMTP) with ESMTPSA 5, 23 -- for {microscopy-at-microscopy.com} ; Sat, 20 Jul 2019 08:34:51 +0300 (EEST) 5, 23 -- From: "Yorgos Nikas" {eikonika-at-otenet.gr} 5, 23 -- To: {microscopy-at-microscopy.com} 5, 23 -- References: {000401d53c8d$3ef6bab0$bce43010$-at-otenet.gr} 5, 23 -- In-Reply-To: {000401d53c8d$3ef6bab0$bce43010$-at-otenet.gr} 5, 23 -- Subject: FW: JSM 5600LV vacuum leak -updated 5, 23 -- Date: Sat, 20 Jul 2019 08:34:58 +0300 5, 23 -- Message-ID: {00cd01d53ebc$dec65010$9c52f030$-at-otenet.gr} 5, 23 -- MIME-Version: 1.0 5, 23 -- Content-Type: text/plain; 5, 23 -- charset="iso-8859-1" 5, 23 -- X-Mailer: Microsoft Outlook 14.0 5, 23 -- Thread-Index: AQK+qNA95FA+3y6VUM6XRLHRMh7Zt6UAI/kg 5, 23 -- Content-Language: en-us 5, 23 -- Content-Transfer-Encoding: 8bit 5, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id x6K5Weg7021341 ==============================End of - Headers==============================
From rosaronald70x-at-gmail.com Sat Jul 20 04:39:49 2019 Return-Path: {rosaronald70x-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.162.207] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x6K9dmWT003944 for {microscopylistserverarchive7-at-microscopy.com} ; Sat, 20 Jul 2019 04:39:48 -0500 Received: from unknown (HELO relay.2yahoo.com) (Fri, 19 Jul 2019 22:23:15 -1100) by mts.locks.grgtween.net with SMTP; Fri, 19 Jul 2019 22:23:15 -1100 Received: from relay.2yahoo.com [204.213.17.84] by relay37.vosimerkam.net with NNFMP; Fri, 19 Jul 2019 22:22:13 -1100 Received: from unknown (HELO smtp.mixedthings.net) (Fri, 19 Jul 2019 22:09:33 -1100) by public.micromail.com.au with ASMTP; Fri, 19 Jul 2019 22:09:33 -1100 Received: from qnx.mdrost.com ([67.208.67.32]) by mmx09.tilkbans.com with SMTP; Fri, 19 Jul 2019 21:54:37 -1100 Received: from unknown (6.91.5.20) by smtp18.yenddx.com with ESMTP; Fri, 19 Jul 2019 21:44:29 -1100 Message-ID: {E14AE061.DA3B3A6B-at-gmail.com}
Dear All,
We have a FEI Talos F200X TEM and I am having trouble measuring the composition and doing a spectrum Imaging (EDS map) of an amorphous film (Si, Mg, Fe-oxide) using EDS. I have tried a large Gun Lens value (STEM mode: Extraction voltage 4500, Gun Lens 6) and large spot size (6-8) but the sample gets destroyed after few minutes of mapping (20-50 microsecond dwell time).
Does anyone have experience in studying beam sensitive samples and the parameters (Extraction voltage, Gun lens, Spot size) that I can use to decrease the damage in the sample before I can get reasonable counts in the EDS map and spectrum. Does using a large spot size 3-4 nm in the STEM mode helps?
I would greatly appreciate your views and suggestions.
The parameters you are trying to change (gun extraction, spot size etc) will only have a marginal impact on beam damage - you have to remember that in order to get an X-ray emission you have to ionise your specimen, and this by definition damage! So to get the counts you want damage may be unavoidable.
I would say that spot 8 and Gun 6 are not normally considered 'large' values for your instrument, and as your increase the extraction voltage you get more electrons (with all other things remaining the same), so you may not be reducing your current as much as you think. Try reducing the extraction (say down at 3800) and increasing to gun 8..
Are the features you want to map on the same scale as your probe size? If you are mapping large (nm scale) features with an sub nm probe you may get some joy spreading the beam (as you suggest), but I have not seen this work very often
However may get more mileage to minimise the impact of beam damage by trying lower voltage - run at 80, 100 or 120kV. This may work if you are limited due to knock on damage. Note however that in some cases this will make damage WORSE, when the damage mode is dominated by electron beam heating (cross section increases with lower voltage). If this is the case then alternative strategies could be to cool the specimen with LN2 or especially if your material is a non-conductor, coat the exit surface (bottom) with a very thin (1 nm if possible) carbon coating - this will provide a conduction path for heat/charge. Since I presume you are already at 200kV on your Talos 200X you don't have the option of increasing the voltage - but 300kV can be much better then 200kV in these cases..
The main message is that beam damage is completely specimen dependent, as such the usual approach is simply to try a number of options.
Good luck,
Matthew
} Dear All, } } We have a FEI Talos F200X TEM and I am having trouble measuring the } composition and doing a spectrum Imaging (EDS map) of an amorphous } film (Si, Mg, Fe-oxide) using EDS. I have tried a large Gun Lens value } (STEM mode: Extraction voltage 4500, Gun Lens 6) and large spot size } (6-8) but the sample gets destroyed after few minutes of mapping } (20-50 microsecond dwell time). } } Does anyone have experience in studying beam sensitive samples and the } parameters (Extraction voltage, Gun lens, Spot size) that I can use to } decrease the damage in the sample before I can get reasonable counts } in the EDS map and spectrum. Does using a large spot size 3-4 nm in } the STEM mode helps? } } I would greatly appreciate your views and suggestions. } } Best regards } } Surya Rout } } } ----------------------------------------------------------------------------------
-- Dr Matthew Weyland Associate Professor & Titan Manager
Monash Centre for Electron Microscopy and Department of Materials Science and Engineering 10 Innovation Walk, Clayton Campus Monash University Clayton VIC 3800 Australia
Thank you very much for all the very helpful suggestions on reducing the damage to my samples due to electron irradiation.
Best regards Surya
Zitat von Surya Snata Rout {surya.rout-at-tuhh.de} :
} Dear All, } } We have a FEI Talos F200X TEM and I am having trouble measuring the } composition and doing a spectrum Imaging (EDS map) of an amorphous } film (Si, Mg, Fe-oxide) using EDS. I have tried a large Gun Lens } value (STEM mode: Extraction voltage 4500, Gun Lens 6) and large } spot size (6-8) but the sample gets destroyed after few minutes of } mapping (20-50 microsecond dwell time). } } Does anyone have experience in studying beam sensitive samples and } the parameters (Extraction voltage, Gun lens, Spot size) that I can } use to decrease the damage in the sample before I can get reasonable } counts in the EDS map and spectrum. Does using a large spot size 3-4 } nm in the STEM mode helps? } } I would greatly appreciate your views and suggestions. } } Best regards } } Surya Rout } } } ---------------------------------------------------------------------------------- } } } Dr. Surya Snata Rout } M-26, Betriebseinheit Elektronenmikroskopie } Technische Universität Hamburg } (Hamburg University of Technology) } Eißendorfer Straße 42 } 21073 Hamburg } Germany } } Tel.: +49 (0) 40 - 428 78 4881
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Title-Subject: [Filtered] STEM postdoc and phd position available
Message: Dear All,
I have openings for a postdoc position and a phd position involving the development and application of electron ptychography in STEM, including for use at ultra low doses. These positions are situated in the Electron microscopy for Materials science (EMAT) group at the University of Antwerp, Belgium. Links to the full job ads below.
postdoc: http://nano.uantwerpen.be/jobs/2019/05/06/postdoc-position-in-the-area-of-high-definition-electron-microscopy-greater-clarity-via-multidimensionality-hdem/ or http://tiny.cc/HDEM_postdoc
and the phd position: http://nano.uantwerpen.be/jobs/2019/04/29/phd-position-in-the-area-of-high-definition-electron-microscopy-greater-clarity-via-multidimensionality-hdem/ or http://tiny.cc/HDEM_phd
Timothy Pennycook EMAT, University of Antwerp Faculty of Science / Department of Physics Campus Groenenborger - U.431 Groenenborgerlaan 171 - 2020 Antwerp, Belgium Timothy.Pennycook-at-uantwerpen.be http://emat.uantwerpen.be
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Title-Subject: [Filtered] Postdocroral position available at Northwestern University
Message: Colleagues, We are looking for a postdoctoral research associate for “Characterizing Structure and Dynamics of Soft Matter with multimodal Electron Microscopy”. Objects of interest include engineered proteins, megamolecules, protein complexes, Metal-Organic and Covalent Frameworks (MOFs/COFs) and soft-hard interfaces. The candidate will be a member of the group of Professor Vinayak Dravid in the Department of Materials Science & Engineering and participate in a cross-disciplinary and collaborative project across Chemistry, Biomedical Engineering, and Cell & Molecular Biology experts at Northwestern and the University of Chicago. We are looking for applicants with hands-on experience in cryo-EM of proteins and in data-processing for the reconstruction of molecular structure. Please send applications with introduction letter, CV, research statement (1 page), and contact information for 3 references (name, postal & email address, phone number) as a single PDF to amy.morgan-at-northwestern.edu and v-dravid-at-northwestern.edu.
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From florjone348iyypi-at-gmail.com Wed Jul 24 20:25:24 2019 Return-Path: {florjone348iyypi-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.162.200] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x6P1PMDD021571 for {microscopylistserverarchive7-at-microscopy.com} ; Wed, 24 Jul 2019 20:25:23 -0500 Message-ID: {BB2FDB95.6E8B6893-at-gmail.com}
Dear Colleagues,
If you are going to the M&M meeting in Portland next week, please mark in your calendar that the Diagnostic & Biomedical Microscopy Focus Interest Group (DBM-FIG, formerly Diagnostic Microscopy) will be hosting a social gathering on Wednesday 8/7, 5 to 7 PM at the BLVD Kitchen of the Marriott Courtyard Downtown/Convention Center Hotel (435 NE Wasco St.). DBM-FIG is the organizer of the symposium B03 “Utilizing Microscopy for Research and Diagnosis of Diseases in Humans, Plants and Animals”. Please come to our symposium and meet with us and all the presenters on Wednesday evening. Refreshments will be served and are kindly provided by Microscopy Innovations (mPrep capsules and ASP-1000 automated specimen processor). Looking forward to seeing you in Portland.
Best regards, Ru-ching Hsia University of Maryland Baltimore Associate Professor and Director of Electron Microscopy Core Imaging Facility Leader, Diagnostic & Biomedical Microscopy Focus Interest Group, 2019-2020
we are looking for a used but fully functional Gatan 626, 914 or a CT3500 cryo-transfer holder (FEI version). Please let me know if you have one that you do not need any longer and are willing to sell.
Best regards, Benedikt Haas
-- Dr. Benedikt Haas Humboldt-Universität zu Berlin Department of Physics Structure Research & Electron Microscopy Group Newtonstr. 15 12489 Berlin, Germany Tel: +49 (0)30 2093-7937 https://www.physik.hu-berlin.de/sem
==============================Original Headers============================== 5, 22 -- From haas-at-physik.hu-berlin.de Fri Jul 26 07:31:33 2019 5, 22 -- Received: from irz6a.physik.hu-berlin.de (irz6a.physik.hu-berlin.de [141.20.40.56]) 5, 22 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x6QCVWlM008929 5, 22 -- for {microscopy-at-microscopy.com} ; Fri, 26 Jul 2019 07:31:33 -0500 5, 22 -- Received: from roundcube1.physik.hu-berlin.de (roundcube1.physik.hu-berlin.de [141.20.40.66]) 5, 22 -- (using TLSv1.2 with cipher ECDHE-RSA-AES256-GCM-SHA384 (256/256 bits)) 5, 22 -- (No client certificate requested) 5, 22 -- by irz6a.physik.hu-berlin.de (Postfix) with ESMTPSA id 4AA1E3E263 5, 22 -- for {microscopy-at-microscopy.com} ; Fri, 26 Jul 2019 14:34:05 +0200 (CEST) 5, 22 -- X-Virus-Status: Clean 5, 22 -- X-Virus-Scanned: clamav-milter 0.100.3 at irz6a 5, 22 -- MIME-Version: 1.0 5, 22 -- Content-Type: text/plain; charset=UTF-8; 5, 22 -- format=flowed 5, 22 -- Content-Transfer-Encoding: 8bit 5, 22 -- Date: Fri, 26 Jul 2019 14:34:05 +0200 5, 22 -- From: Benedikt Haas {haas-at-physik.hu-berlin.de} 5, 22 -- To: microscopy-at-microscopy.com 5, 22 -- Subject: TEM - Looking for FEI Cryo Holder 5, 22 -- Message-ID: {625d0e0d93444335fdb708588c24426b-at-physik.hu-berlin.de} 5, 22 -- X-Sender: haas-at-physik.hu-berlin.de 5, 22 -- User-Agent: Roundcube Webmail/1.3.9 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both levenson-at-ucdavis.edu, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Email: levenson-at-ucdavis.edu Name: Richard Levenson
Organization: UC Davis Health
Title-Subject: [Filtered] Job posting for adjunct assistant professor in pathology imaging
Message: We are seeking applications for a new position as adjunct assistant professor in the departent of Pathology and Laboratory Medicine at UC Davis Health, Sacramento, CA, USA.
Link to job description here, which has its focus on tissue imaging.
https://bit.ly/2Yq33Uq
Thanks.
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Email: levenson-at-ucdavis.edu Name: Richard Levenson
Organization: UC Davis Health
Title-Subject: [Filtered] Job position, adjunct assistant professor
Message: We are seeking applications for a new position as adjunct assistant professor in the department of Pathology and Laboratory Medicine at UC Davis Health, Sacramento, CA, USA.
Link to job description here, which has its focus on tissue imaging.
https://bit.ly/2Yq33Uq
Thanks.
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From fritschemichael755degok-at-gmail.com Sun Jul 28 19:46:50 2019 Return-Path: {fritschemichael755degok-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.162.195] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x6T0knhw001247 for {microscopylistserverarchive7-at-microscopy.com} ; Sun, 28 Jul 2019 19:46:50 -0500 Received: from [72.197.55.90] by mx.reskind.net with ESMTP; Sun, 28 Jul 2019 16:39:12 -0800 Received: from relay-x.misswldrs.com [104.171.179.198] by mail.gimmicc.net with ESMTP; Sun, 28 Jul 2019 16:38:23 -0800 Message-ID: {3E5C6F10.94E34D8E-at-gmail.com}
X-from: fei.long-at-queensu.ca
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Email: fei.long-at-queensu.ca Name: Fei Long
Organization: Queen's University
Title-Subject: [Filtered] SEM EDX repairing experts
Message: Hi all,
Does anyone know who is expert in SEM EDX repairing? Our Bruker XFlash EDS system stopped working recently so if anyone has hands on experiences with it I will be very happy to contact to inquire quote for repairing.
Best regards,
Fei
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Email: jhyun-at-gatan.com Name: John Hyun
Organization: Gatan, Inc.
Title-Subject: [Filtered] EELS & EFTEM Analysis Training School - October 2019
Message: October 22-25, 2019 Gatan R&D Headquarters, Pleasanton, CA USA
This is the premiere EELS and EFTEM training program in the world. It is suited for all levels of EELS users.
As a course attendee, you will learn best practices to set up and optimize your EELS hardware and experimental protocols so you can capture and extract the maximum amount of compositional and chemical information from your TEM samples. Topics include:
•Fundamentals of EELS and energy-filtered imaging in TEM •Principles of operation of EFTEM and EELS systems •Optimization of EFTEM and EELS data acquisition •Quantification of elemental composition •Other information provided by EFTEM/EELS and how best to extract it •Use of EELS signals to form maps of elemental and chemical composition •EFTEM and STEM EELS spectrum imaging techniques •Identification of material phases via EELS fine structure mapping •Applications to biological and physical science specimens
If interested, please register online at: http://www.gatan.com/company/events/eels-eftem-analysis-training-school-october-2019
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From isendana0v-at-gmail.com Tue Jul 30 07:25:10 2019 Return-Path: {isendana0v-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.162.203] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x6UCP7VU005253 for {microscopylistserverarchive7-at-microscopy.com} ; Tue, 30 Jul 2019 07:25:08 -0500 Message-ID: {65A812BF.EE36228C-at-gmail.com}
J. Kraft Microscopy Services provides quality service to the electron microscopy industry as an independent service provider.
We are seeking an experienced sales person with a strong electron microscopy background for a challenging position selling SEM, TEM and ancillary equipment. The following position can be based anywhere in the United States.
Major duties
· Selling new and refurbished scanning electron microscopes and related instrumentation to prospective customers. · Selling and renewing service contracts for scanning electron microscopes and related instrumentation to new and existing customers. · Lead all sales related activities for the US and Canada. · Recruit, manage and develop sales channels. · Maintain website content and marketing materials. · Close cooperation with company staff to achieve company targets. · Follow up on sales leads to completion of order. · Generate sample data reports for sales support and product presentations. · Conduct sales in person and via phone, email and web based meetings. · Participate in marketing events such as trade exhibitions, conferences, and user workshops. · Conduct technical presentations at customer meetings. · Track sales leads and forecast projected sales on a routine basis. · Identify potential additional product sets to add to our portfolio.
Requirements · Degree or post graduate degree in physics, chemistry, biophysics, material science, engineering or similar field. A combination of relevant education and work experience may be considered. · Strong background in electron microscopy (SEM, TEM, and FIB), Energy Dispersive Spectroscopy (EDS) and related detectors and instrumentation. · Hands on experience with above mentioned equipment is desirable. · Prior sales experience in a related field. · Excellent communication skills, fluent in English (written and spoken). · Ability to work independently with support from HQ. · Ability to work well with others, to cooperate and to share success with the whole team. · Willingness to travel in the U.S., and occasionally internationally. · Legal status to be employed and work in the U.S., and possess a valid U.S. driver’s license and passport.
We offer a challenging career in the growing field of electron microscopy, a work environment that is collaborative, flexible and highly professional. You will join a team of professionals that is dynamic, innovative and ready to facilitate customers as needed.
Interested parties should reply to this posting by electronic response, phone calls will not be accepted. Replies can be sent to jkraft-at-jkraftmicro.com.
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Email: kunli218-at-yahoo.com.sg Name: kun Li
Organization: King Abdullah University of Science and Technology
Title-Subject: [Filtered] Two TEM staff scientist openings
Message: The Imaging and Characterization Core Lab (IAC) at King Abdullah University of Science and Technology (KAUST) is equipped with state-of-the-art electron microscopy facility and is seeking highly motivated transmission electron microscopy scientists with strong background in advanced monochromated and spherical aberration corrected analytical transmission electron microscopy, high resolution TEM imaging and simulation, and/or electron crystallography. The EM facility is a strong enabler for KAUST research, contributing to numerous high impact publications in top tier journals, such as Science, Nature Materials and Nature Chemistry. The scientists would serve the IAC mission to support research at KAUST and regionally by providing advanced techniques and expertise in transmission electron microscopy within a user- driven environment. He or she will be part of a team of scientists and specialists in the EM unit and will fulfill a multitude of laboratory and university-related tasks.
Major Responsibilities • Apply advanced TEM techniques (Cs-corrected TEM/STEM, Monochromated TEM/STEM, HRTEM/STEM simulation, electron crystallography, and/or scripting) to solve challenging scientific problems • Develop new TEM techniques/methodologies/analytical protocols to meet the challenges in a multi-user environment • Provide TEM training to researchers, students and other lab staff • Engage in KAUST research projects that require advanced EM techniques.
Qualifications & Competencies • Ph.D. degree in materials science, physics or related disciplines with solid background in electron microscopy. • Minimum 3 years of relevant working experience. Fresh Ph.Ds with Ph.D work focusing on TEM are also welcome. • Expertise in Cs-corrected TEM, Monochromated TEM, electron crystallography, HR-TEM/STEM simulation, or Digital Micrograph Scripting (or electron microscopy-related programming) • Good communication and inter-personal skills. • Excellent written and oral communication skills of English.
If interested, please send your CV to kun.li-at-kaust.edu.sa.
If you are attending the coming M&M at Portland, you can arrange a face to face discussion with Dr. Kun Li, the IAC Lab Director through the above email address.
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Dear colleagues - in conjunction with the next week MSA meeting, I would like to bring your attention to a new approach for code and data sharing in scientific publications enabled via Jupyter notebooks in Google Colabs. You can find the information on these Jupyter paper in the following video (narrated by Maxim Ziatdinov) on the YouTube channel "M*N: Microscopy, Machine Learning, Materials":
In some recent makes of 120kV TEMs manufacturers have chosen to have oil-free (no ODPs) vacuum systems. In one case it is scrolling pumps and turbo-molecular pumps, in the other case it is rotary pump, turbo-molecular pump and 2 IGPs (one for column and one for gun area).
How beneficial these systems in practical terms in regard to standard resin sections routine TEM? Room temperature STEM? X-ray microanalysis?
There were mentions somewhere that ODP can contaminate CCD camera chip but in our experience with T12 and Orius Gatan camera there were no noticeable image quality degradation for 10 years.
In practice, will getting rid of ODPs benefit to any biological TEM system?
Sincerely,
Alex
-- Dr. Aleksandr Mironov MD, PhD Senior Experimental Officer D.1527, M.Smith Building EM Core Facility School of Biological Sciences, Faculty of Biology Medicine and Health University of Manchester Oxford Road Manchester M13 9PT UK
Looking for a job that will make a difference? Research Laboratory Specialist in the Division of Light Microscopy, Cell & Tissue Imaging Center will work with a dynamic team of imaging scientists to offer expert training and advice on design and execution of imaging experiments to investigators conducting transformative research at St. Jude. Along with other staff, you will consult with St. Jude scientists planning to use microscopy in their research; assist with image acquisition, analysis, quantification, presentation, and publication; and educate investigators on the theory and practice of the resident technologies.
An overview of the Cell & Tissue Imaging Center, Light Microscopy Division may be found at: https://www.stjude.org/research/shared-resources/cell-and-tissue-imaging-center/light-microscopy-core-facility.html
Responsibilities The successful candidate will: * Possess a working knowledge of modern microscopy techniques and willingness to learn new technologies. * Demonstrate an analytical approach to designing experiments and applying appropriate imaging techniques to answer biological research questions. * Approach duties with attention to detail and keep comprehensive records. * Possess outstanding organizational skills and ability to manage multiple projects. * Demonstrate excellent oral and written communication skills. * Perform as a team player excited to collaborate with all St. Jude colleagues. * Apply the highest standards of integrity and respect.
Training opportunities will be provided to enhance your expertise and continuing education in response to the latest developments in imaging technology.
Minimum Education * Bachelor's degree in appropriate scientific field is required. Minimum Experience * A minimum of eight (8) years of relevant post-degree work experience, with at least five (5) years at the Sr. Research Tech level, is required with a Bachelor's degree * A minimum of seven (7) years of relevant post-degree work experience, with at least (5) years at the Sr. Research Tech level, is required with a Master's degree * A minimum of four (4) years of relevant work experience including post-doctoral and/or technical staff experience is required with a Ph.D * BS/MS staff at the Senior Research Technologist level as of 5/12/2003 could be considered for promotion to Research Lab Specialist after five (5) years as a SJCRH Senior Research Technologist or an equivalent position in a similar research environment.
} On Jul 31, 2019, at 8:28 AM, Aleksandr.Mironov-at-manchester.ac.uk wrote: } } Dear Listers, } } In some recent makes of 120kV TEMs manufacturers have chosen to have } oil-free (no ODPs) vacuum systems. In one case it is scrolling pumps and } turbo-molecular pumps, in the other case it is rotary pump, } turbo-molecular pump and 2 IGPs (one for column and one for gun area). } } How beneficial these systems in practical terms in regard to standard } resin sections routine TEM? Room temperature STEM? X-ray microanalysis? } } There were mentions somewhere that ODP can contaminate CCD camera chip } but in our experience with T12 and Orius Gatan camera there were no } noticeable image quality degradation for 10 years. } } In practice, will getting rid of ODPs benefit to any biological TEM system? } } Sincerely, } } Alex } } -- } Dr. Aleksandr Mironov MD, PhD } Senior Experimental Officer } D.1527, M.Smith Building } EM Core Facility } School of Biological Sciences, } Faculty of Biology Medicine and Health } University of Manchester } Oxford Road } Manchester } M13 9PT } UK } Hi Aleksandr, The better gun vacuum obtainable with ion pumps is especially worthwhile if you have a LaB6 filament. Better column vacuum is never bad (AFAIK), but for resin sections is not usually crucial. If you’re considering replacing the vacuum system, it might be more cost-effective to concentrate on gun vacuum. If you’re contemplating getting a new scope, go for one with TMPs and IPs, A more important consideration for STEM and EDX is likely to be source size. Yours, Bill
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Email: rvancamp-at-kettering.edu Name: Rick
Organization: Kettering University
Title-Subject: [Filtered] Need Pirani Gauge O-ring Dimension for JEOL 2100 series
Message: I discovered a compromised o-ring on one of the Pirani gauges on our TEM. I do not know the size or part number. Is anyone able to help me with the correct dimensions and part number?
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Title-Subject: [Filtered] Chiller Replacement on T12 TEM
Message: Our Haskris R075 water-cooled chiller for our Thermo/FEI T12 TEM needs to be replaced. Has anyone switched and/or have feedback on a air-cooled chillers? We would love to be able to bypass our university chilled water if there aren't any drawbacks that we can't live with. I've gathered that the air-cooled are a bit noisier...
Melissa Chimento | Lab Manager High Resolution Imaging Shared Facility UAB | The University of Alabama at Birmingham SHEL 135 | 1825 University Boulevard | Birmingham, AL 35294-0107 P: 205.934.1926 |F:205.934.1928 |email: mchimento-at-uab.edu http://www.uab.edu/hrif
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} Message: Our Haskris R075 water‑cooled chiller for our Thermo/FEI T12 TEM } needs to be replaced. Has } anyone switched and/or have feedback on a air‑cooled chillers? We would love
} to be able to bypass } our university chilled water if there aren't any drawbacks that we can't } live with. I've gathered } that the air‑cooled are a bit noisier...
We have done this - once. We will not do this, again. We even shipped the air-cooled version to the manufacturer to get it rebuilt to the water-cooled version ... successfully. Here: In a hot summer, the room where this this air-cooled chiller was positioned, the temperature was above 30°C (no air-conditioning possible!!), and thus, the chiller was not able to provide the water temperature (18 C) needed / desired for the TEM (in fact, for the CM12, i.e. the earlier version of the T12). In the case, the room temperature with the chiller is tightly controlled, ideally, air-conditioned: you may consider this air-cooled version.... But, yes: the air-cooled version is (a) cheaper, (b) producing a lot of "warm air" (logically), and (c) noisier than the water-cooled version. as usual: it depends ... (I can understand why you want to bypass the University's chilled water ... - here, we have water filters in front of the chiller input, in order to prevent the dirty Univ.chilled water to block the heat exchanger inside the chiller ... )
kind regards, Reinhard
-- Prof. Dr. Reinhard Rachel University of Regensburg Centre for EM / Anatomy Faculty of Biology & Preclin. Med. Universitaetsstrasse 31 D-93053 Regensburg - Germany tel +49 941 943 -2837, -1720 mail reinhard.rachel-at-biologie.uni-regensburg.de office: VKL 3.1.29 member of the IFSM board
Next microscopy conferences: - Microscopy Conference MC2019, 1.-5. Sept 2019 in Berlin - EMC2020 in Kopenhagen, 23.-28.8. 2020 (European conference) - MC2021 in Vienna (D-A-CH conference) - next Microbiol. conferences: VAAM 8.-11.03. 2020 Leipzig
The Center for Electron Microscopy and Analysis (CEMAS) at Ohio State University has several openings available. There is a permanent staff position for a Research Associate in Cryo-EM and several postdoctoral positions. One is in micro-ED and the second is nanoscale materials interface morphology. Additional post-doctoral positions may open soon. Stay tuned!
The details and application information are at https://cemas.osu.edu/about/employment-opportunities
CEMAS is pushing the frontiers at the boundary of life and materials sciences with a multidisciplinary approach to challenge the possibilities of characterization.
Henk
----------------
Hendrik O. Colijn Center for Electron Microscopy and AnalysiS The Ohio State University 1305 Kinnear Rd, Suite 100
At ASU, we had a water-to-water heat exchanger so that the university’s dirty water did not go through the chillers.
A. John Mardinly, Ph.D., P.E.
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Has } } anyone switched and/or have feedback on a air‑cooled chillers? We } } would love } } } to be able to bypass } } our university chilled water if there aren't any drawbacks that we can't } } live with. I've gathered } } that the air‑cooled are a bit noisier... } } We have done this - once. We will not do this, again. We even shipped } the air-cooled version to the } manufacturer to get it rebuilt } to the water-cooled version ... successfully. } Here: In a hot summer, the room where this this air-cooled chiller was } positioned, the temperature was above 30°C (no air-conditioning } possible!!), } and thus, the chiller was not able to provide the water temperature (18 C) } needed / desired for the TEM (in fact, for the CM12, i.e. the earlier } version } of the T12). } In the case, the room temperature with the chiller is tightly controlled, } ideally, air-conditioned: you may consider this air-cooled version.... } But, yes: the air-cooled } version is (a) cheaper, (b) producing a lot of "warm } air" (logically), and (c) noisier than the water-cooled version. } as usual: it depends ... (I can understand why you want to bypass the } University's chilled water ... - here, we have water filters in front } of the } chiller input, in order to prevent the dirty Univ.chilled water to } block the } heat exchanger inside the chiller ... ) } } kind regards, } Reinhard } } -- } Prof. Dr. Reinhard Rachel } University of Regensburg } Centre for EM / Anatomy } Faculty of Biology & Preclin. Med. } Universitaetsstrasse 31 } D-93053 Regensburg - Germany } tel +49 941 943 -2837, -1720 } mail reinhard.rachel-at-biologie.uni-regensburg.de } {mailto:reinhard.rachel-at-biologie.uni-regensburg.de} } office: VKL 3.1.29 member of the IFSM board } } Next microscopy conferences: } - Microscopy Conference MC2019, 1.-5. 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Melissa, Air cooled chillers present their own problems (extreme heat and extra noise). I've seen them work when the microscope room shared an outside wall (I believe the outside-location chiller will suffer more wear), and I had one installed in a penthouse directly above the TEM microscope. The heat is pretty extreme, though.
We were running a chiller using city water at our previous, 100 year old building location. You'll always need a pre-filter whether using city water (mostly rust) or university water (mystery black substance). When using city water, you'll need a drain for the chiller to dump the warmed water. I'm not sure if anyone over here determined how much water was dumped during normal operations. It was a legacy system when I came on board.
Good luck! ~Gregg *Gregg Sobocinski* Microscope Imaging Suite, Managing Director University of Michigan, MCDB Dept. Ann Arbor, Michigan
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Title-Subject: [Filtered] Chiller Replacement on T12 TEM
Message: Our Haskris R075 water-cooled chiller for our Thermo/FEI T12 TEM needs to be replaced. Has anyone switched and/or have feedback on a air-cooled chillers? We would love to be able to bypass our university chilled water if there aren't any drawbacks that we can't live with. I've gathered that the air-cooled are a bit noisier...
Melissa Chimento | Lab Manager High Resolution Imaging Shared Facility UAB | The University of Alabama at Birmingham SHEL 135 | 1825 University Boulevard | Birmingham, AL 35294-0107 P: 205.934.1926 |F:205.934.1928 |email: mchimento-at-uab.edu {mailto:mchimento-at-uab.edu} http://www.uab.edu/hrif
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Here at UCLA we have the water chiller hooked up to the house chilled water to cool the compressor. Odd pink water they have here. It was piped to have a inflow and outflow for the university water system. It has work for quite a while on both our older EM208S scope (20 years) and out Tecnai G2 (3 years). "You only get one sunrise and one sunset a day, and you only get so many days on the planet. A good photographer does the math and doesn’t waste either." Galen Rowell
"Now this is not the end. It is not even the beginning of the end. But it is, perhaps, the end of the beginning." Winston Churchill Eric Rosen The Desert Rat.. This Message made with Recycled Electrons!
} On Aug 2, 2019, at 5:13 PM, microscopy.listserver-at-gmail.com wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } } } -------- Forwarded Message -------- } } } } At ASU, we had a water-to-water heat exchanger so that the university’s } dirty water did not go through the chillers. } } A. John Mardinly, Ph.D., P.E. } } } } On Aug 2, 2019, at 4:46 AM, microscopy.listserver-at-gmail.com } } {mailto:microscopy.listserver-at-gmail.com} wrote: } } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- } } https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.com_MicroscopyListserver&d=DwIBaQ&c=l45AxH-kUV29SRQusp9vYR0n1GycN4_2jInuKy6zbqQ&r=VLPJ8OE-c_C6joGeE1ftlvxMmQPq9N6mpKZONBRt90E&m=kz3IlPyruMnmeEEhQWJ81tO8eSR7gA69R3wG-m4Gf5k&s=IHxhR5BW0PghDSJgzZe8aoCPmeHqU9kr8UpqkJWrIh8&e= } } } } On-Line Help } } https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.com_MicroscopyListserver_FAQ.html&d=DwIBaQ&c=l45AxH-kUV29SRQusp9vYR0n1GycN4_2jInuKy6zbqQ&r=VLPJ8OE-c_C6joGeE1ftlvxMmQPq9N6mpKZONBRt90E&m=kz3IlPyruMnmeEEhQWJ81tO8eSR7gA69R3wG-m4Gf5k&s=OCAdxiX_HCRufeRJFtyrfXlUTRqRcHpgGsr_X0xkjfw&e= } } } } ---------------------------------------------------------------------------- } } } } } }
At The KIT I use for 25 years now an air cooled "Van der Heiyden KĂĽhlmobil" water chiller in series with an heat exchanger cooled by the house chilled water. The chiller has a simple modification: The compessor is controlled by an external thermal switch. In normal operation water from the microscope is chilled by the heat exchanger. If the temperature at the outlet of the heat exchanger is low enough, the compressor of the chiller is not working. The chiller only pumps the water to the microscope. If the temperature at the outlet of the heat exchanger is to high, the thermal switch starts the compressor (for 24 hours to avoid switch on-switch off) and water is chilled by the air cooled chiller. Best wishes Winfried
PS: Never place the air cooled chiller in the microscope room. Air cooled chillers should be placed in an environment with low ambient temperature (basement...)
Am Sa., 3. Aug. 2019 um 04:43Â Uhr schrieb {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } :
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Here at UCLA we have the water chiller hooked up to the house chilled water to cool the compressor. Odd pink water they have here.  It was piped to have a inflow and outflow for the university water system. It has work for quite a while on both our older EM208S scope (20 years) and out Tecnai G2 (3 years). "You only get one sunrise and one sunset a day, and you only get so many days on the planet. A good photographer does the math and doesn’t waste either." Galen Rowell
"Now this is not the end. It is not even the beginning of the end. But it is, perhaps, the end of the beginning." Winston Churchill Eric Rosen The Desert Rat.. This Message made with Recycled Electrons!
} On Aug 2, 2019, at 5:13 PM, microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} wrote: } } } } }
---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html }
---------------------------------------------------------------------------- } } } } } -------- Forwarded Message -------- } } } } At ASU, we had a water-to-water heat exchanger so that the university’s } dirty water did not go through the chillers. } } A. John Mardinly, Ph.D., P.E. } } } } On Aug 2, 2019, at 4:46 AM, microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } } {mailto:microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote: } } } } } } } } } }
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Title-Subject: [Filtered] Denton Desktop II Manual
Message: Anyone have an operations/maintenance manual they would share?
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Title-Subject: [Filtered] EMS Microscopy Academy releases the 2020 Course List
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Biological TEM: A Complete Picture January 14 - 16, 2020 Hatfield, PA Sponsor: Electron Microscopy Sciences www.emsmicroscopyacademy.com/product-page/biotem-jan20
X-Ray Microanalysis January 21 – 23, 2020 Hatfield, PA Sponsor: Electron Microscopy Sciences www.emsmicroscopyacademy.com/product-page/xray-jan20
Automated and Rapid Specimen Processing for Electron Microscopy February 18 - 20, 2020 Hatfield, PA Sponsor: Electron Microscopy Sciences www.emsmicroscopyacademy.com/product-page/auto-feb20
Materials Ultramicrotomy February 24, 2020 (Beginner) February 25 - 27, 2020 (Some Experience) Hatfield, PA Sponsor: Electron Microscopy Sciences www.emsmicroscopyacademy.com/product-page/materials-feb20
Introduction to Microscopy Techniques March 3 - 5, 2020 Hatfield, PA Sponsor: Electron Microscopy Sciences www.emsmicroscopyacademy.com/product-page/intro-mar20
Biological SEM March 17 - 19, 2020 Hatfield, PA Sponsor: Electron Microscopy Sciences www.emsmicroscopyacademy.com/product-page/biosem-mar20
Cryo SEM March 24 - 26, 2020 Hatfield, PA Sponsor: Electron Microscopy Sciences www.emsmicroscopyacademy.com/product-page/cryosem-mar20
Aurion Immunogold Silver Staining April 15 - 17, 2020 Hatfield, PA Sponsor: Electron Microscopy Sciences www.emsmicroscopyacademy.com/product-page/immunogold-apr20
Cryosectioning/Immunogold April 20 - 24, 2020 Hatfield, PA Sponsor: Electron Microscopy Sciences www.emsmicroscopyacademy.com/product-page/cryoimmuno-apr20
Introduction to Microscopy Techniques May 19 - 21, 2020 Hatfield, PA Sponsor: Electron Microscopy Sciences www.emsmicroscopyacademy.com/product-page/intro-may20
X-Ray Microanalysis May 26 - 28, 2020 Hatfield, PA Sponsor: Electron Microscopy Sciences www.emsmicroscopyacademy.com/product-page/xray-may20
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Introduction to Microscopy Techniques September 15 - 17, 2020 Hatfield, PA Sponsor: Electron Microscopy Sciences www.emsmicroscopyacademy.com/product-page/intro-sep20
Materials Ultramicrotomy September 21, 2020 (Beginner) September 22 - 24, 2020 (Some Experience) Hatfield, PA Sponsor: Electron Microscopy Sciences www.emsmicroscopyacademy.com/product-page/materials-sep20
Cryo SEM October 13 - 15, 2020 Hatfield, PA Sponsor: Electron Microscopy Sciences www.emsmicroscopyacademy.com/product-page/cryosem-oct20
Biological SEM October 20 - 22, 2019 Hatfield, PA Sponsor: Electron Microscopy Sciences www.emsmicroscopyacademy.com/product-page/biosem-oct20
Aurion Immunogold Silver Staining November 10 - 12, 2020 Hatfield, PA Sponsor: Electron Microscopy Sciences www.emsmicroscopyacademy.com/product-page/immunogold-nov20
Biological TEM November 17 - 19, 2020 Hatfield, PA Sponsor: Electron Microscopy Sciences www.emsmicroscopyacademy.com/product-page/biotem-nov20
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Email: joseph.mowery-at-ars.usda.gov Name: Joe Mowery
Can anyone recommend a research grade Shore D durometer for measuring the hardness of TEM resin blocks, preferably under $500.
Best regards, -Joe
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If you are only need to access condition of your resin blocks after the curing, then there are plenty of digital durometers in $50 range available on Amazon. Research-grade instrument with NIST certificate would set you back around $1,2000 and high-end stand would add another $300 or so - all together is about x3 above the $500 budget. Check www.durometer.com for some high-end instruments.
Valery Ray (also with REFINE Lab, UCONN) ================================ MEO Engineering Company 290 Broadway, Suite 298 Methuen, MA 01844, USA Mobile: +1-978-305-0479 - leave a message https://www.linkedin.com/in/valeryray/ E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
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Email: joseph.mowery-at-ars.usda.gov {mailto:joseph.mowery-at-ars.usda.gov} Name: Joe Mowery
Can anyone recommend a research grade Shore D durometer for measuring the hardness of TEM resin blocks, preferably under $500.
Best regards, -Joe
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I received some ice cream samples for freeze substitution and lowicryl HM20 embedding. My problem is the samples are floating on the surface of substitution cocktail, do not sink into the bottom of the vial. Does anybody have experience with this kind of experiment? How can I keep samples submerged in the solution and embedding medium to get good substitution and then UV polymerization?
If you are only need to access condition of your resin blocks after the curing, then there are plenty of digital durometers in $50 range available on Amazon. Research-grade instrument with NIST certificate would set you back around $1,2000 and high-end stand would add another $300 or so - all together is about x3 above the $500 budget. Check https://nam01.safelinks.protection.outlook.com/?url=www.durometer.com&data=02%7C01%7CEjb63%40drexel.edu%7C94df59bd9ceb45aaeb1f08d7212c0597%7C3664e6fa47bd45a696708c4f080f8ca6%7C0%7C0%7C637014345280882492&sdata=WO0vG3i825EDe98xenX6oC3AowHT4qyw6cYtNFZLcFo%3D&reserved=0 for some high-end instruments.
Valery Ray (also with REFINE Lab, UCONN) ================================ MEO Engineering Company 290 Broadway, Suite 298 Methuen, MA 01844, USA Mobile: +1-978-305-0479 - leave a message https://nam01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.linkedin.com%2Fin%2Fvaleryray%2F&data=02%7C01%7CEjb63%40drexel.edu%7C94df59bd9ceb45aaeb1f08d7212c0597%7C3664e6fa47bd45a696708c4f080f8ca6%7C0%7C0%7C637014345280882492&sdata=VrQoPYZbh7EzR%2BCQbqgGBgv0PH2x2TvWMTgvhqsrSMk%3D&reserved=0 E-mail: vray-at-partbeamsystech.com Web: https://nam01.safelinks.protection.outlook.com/?url=www.partbeamsystech.com&data=02%7C01%7CEjb63%40drexel.edu%7C94df59bd9ceb45aaeb1f08d7212c0597%7C3664e6fa47bd45a696708c4f080f8ca6%7C0%7C0%7C637014345280882492&sdata=ygMpa6fIV8szLa7z0ix3KZO8u9Nm0fbjdl80l8L3Dtg%3D&reserved=0 Web: https://nam01.safelinks.protection.outlook.com/?url=www.freudlabs.com&data=02%7C01%7CEjb63%40drexel.edu%7C94df59bd9ceb45aaeb1f08d7212c0597%7C3664e6fa47bd45a696708c4f080f8ca6%7C0%7C0%7C637014345280892481&sdata=wUYVevOS8CdJB1tAHAyJGK%2BPn3996IOEd6YznGJyBNA%3D&reserved=0
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Email: joseph.mowery-at-ars.usda.gov {mailto:joseph.mowery-at-ars.usda.gov} Name: Joe Mowery
Can anyone recommend a research grade Shore D durometer for measuring the hardness of TEM resin blocks, preferably under $500.
Best regards, -Joe
Joe Mowery | Biologist Electron and Confocal Microscopy Unit USDA Agricultural Research Service joseph.mowery-at-ars.usda.gov {mailto:joseph.mowery-at-ars.usda.gov} Login Host: 199.133.24.7 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
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Some more little things I've come up with. It seems that a MIP in the order of about 10 V can be explained without any surface effects or adsorbed molecules or ions. That means a (non-existent) ideal crystal, where the surface unit cells are exactly the same as the unit cells inside the solid, would have roughly this MIP. This is supported by estimations based on band theory and one can also get similar results using Poisson's equation on a model charge density. This is clearly in line with what Bill Tivol pointed out.
Obviously surface effects and adsorbed species will add to this value.
The thickness-independent contribution to the phase shift shown in the Wanner paper from 2006 is still a mystery to me, but there's one thing I'd like to point out: The smallest thickness they measured is only 1 nm, which is in the order of 5 atom layers I would say. In a C-film that thin I wouldn't even look for anything related to the bulk of the solid since a film that thin essentially consists of two surfaces and not much in between.
All the best, Philip
X-from: 3dem {3dem-bounces-at-ncmir.ucsd.edu} on behalf of Philip Köck {philip.koeck-at-ki.se} Sent: Wednesday, 3 July 2019 10:57:31 To: microscopy-at-microscopy.com {microscopy-at-microscopy.com} ; 3dem-at-ncmir.ucsd.edu {3dem-at-ncmir.ucsd.edu} Subject: Re: [3dem] [Microscopy] mean inner potential
Hi again.
Thanks for all the input.
I've been looking at the papers suggested by Robert Keyse and Benjamin Himes. What Spence seems to say is that the contribution to the MIP which is only due to "electrons spilling out a bit further at the surface of a solid" is only around 0.5 V. The main contribution to the MIP is supposed to be due to adsorbed atoms and molecules.
Bill Tivol quite rightly said that then the MIP would not be a property of a specific material, but would depend a lot on what else is around in the vacuum or what the surface came in contact with. Are there any experiments on that?
I've also heard the opinion that surface adsorbates contribute only little to the MIP and that it's mainly due to the solid itself.
Then there's still the question of a thickness independent contribution to the phase shift. A tripple surface charge layer like - + - with balanced charges could explain that, but where would that come from?
All the best,
Philip
From: Robert Keyse {rok210-at-lehigh.edu} Sent: Wednesday, 19 June 2019 17:02:11 To: Philip Köck; microscopy-at-microscopy.com Subject: Re: [Microscopy] mean inner potential
Hi Philip,
the Saldin & Spence paper: Ultramicroscopy 55 (1994) 397-406 suggests the surface atoms are less confined and spread their valence electrons out into the vacuum creating a surface dipole that differs from inside the material. . Electrons speed up slightly just as they enter a sample (increase in wave-vector) due to this inner potential.
Reference [12] O'Keefe & Spence: Acta Cryst. (1994) A50, 33-45 (J C H Spence would be a good reference to follow generally) O'Keefe and Spence estimate the surface potential is weak and only about 0.5 volts (page 39: a back of an envelope estimate).
On Tue, Jun 18, 2019 at 3:40 AM {philip.koeck-at-ki.se} wrote:
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Thanks for the reference.
I can't make sense of a thickness-independent contribution to the phase shift either. The way I see it even a surface layer of dipoles would lead to a constant MIP and a phase shift proportional to the thickness.
One can think of a simple model: A slab of completely neutral material (made of neutrons) covered in a layer of positive charge and outside that a layer of negative charge that balances the positive charge. The potential inside this slab will be constant and independent of the thickness of the slab.
I wonder if we can get a comment from someone who knows more.
All the best,
Philip
X-from: 3dem {3dem-bounces-at-ncmir.ucsd.edu} on behalf of Benjamin Himes {himes.benjamin-at-gmail.com} Sent: Monday, 17 June 2019 20:45:48 To: 3dem-at-ncmir.ucsd.edu Subject: [3dem] (mean Inner potential) Re: 3dem Digest, Vol 142, Issue 38
Hi Philip,
The mean inner potential (MIP) refers to a total "interaction" potential that is considered a material property. It consists of all the sources contributing to the potential well seen by an imaging electron, including those you suggest (nuclear and electronic contributions.)
Yes, physical changes to the surface via adsorbed matter will directly affect the MIP. I believe the working hypothesis for the source of the "Volta" potential is through heat/exposure related modification of surface adsorbates.
It is also interesting to note that in addition to the electronic character of the object, the surface contributions of adsorbates and heating, there is another thickness independent phase shift (at least for carbon) the source of which I am not clear on. Happy to hear an explanation from anyone in the know : )
Please have a look at this paper where all of the non-Volta contributions are discussed and also measured.
"Electron holography of thin amorphous carbon films: Measurement of the mean inner potential and a thickness-independent phase shift"
doi: j.ultramic.2005.10.004
HTH
Ben
------------------------ Benjamin Himes
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Hi Greg, I am not at all sure whether FS + Embedding in a resin makes any sense with ice cream samples, at all. I would go for anything like cryo-fracture (plus some short freeze-etching / gentle freeze-drying at -80/90/100°C in high vacuum??) , and then go to a cryoSEM (cryo-shuttle), or - if available - a final coating with carbon and/or Pt/C (at or below -100°C) , i.e. like freeze-etching any other bio-sample. Exactly like Debby's suggestion ... HTH.
kind regards,
Reinhard ============================
} } } 16.08.2019 um 02:31: } I am not sure if you need SEM or TEM but I have had excellent results with } cryo-SEM and ice cream. } Debby } } Debra Sherman, Founder & CSO } DS Imaging LLC } www.dsimagingllc.com } } } On 8/15/19, 7:09 PM, "microscopy.listserver-at-gmail.com" } {microscopy.listserver-at-gmail.com} wrote } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } X-from: Ning, Gang {gxn7-at-psu.edu} } Hi all, } I received some ice cream samples for freeze substitution and } lowicryl HM20 embedding. My } problem is the samples are floating on the surface of substitution } cocktail, do not sink into } the bottom of the vial. Does anybody have experience with this kind of } experiment? How can I } keep samples submerged in the solution and embedding medium to get good
} substitution and then UV } polymerization? } Thank you! } Greg
-- Prof. Dr. Reinhard Rachel University of Regensburg Centre for EM / Anatomy Faculty of Biology & Preclin. Med. Universitaetsstrasse 31 D-93053 Regensburg - Germany tel +49 941 943 -2837, -1720 mail reinhard.rachel-at-biologie.uni-regensburg.de office: VKL 3.1.29 member of the IFSM board
Next microscopy conferences: - Microscopy Conference MC2019, 1.-5. Sept 2019 in Berlin - EMC2020 in Kopenhagen, 23.-28.8. 2020 (European conference) - MC2021 in Vienna (D-A-CH conference) - next Microbiol. conferences: VAAM 8.-11.03. 2020 Leipzig
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Use a dense open-pore polymer sponge/stopper (like that used to cap Drosophila culture bottles). Put the sample in the solution, push in the stopper until it pushes the sample below the solution surface. The sponge will allow the solution to come in contact with the sample surface touching the stopper, insuring complete infiltration. This can be done for all steps of the processing, but you'll have to change the stopper along the way, otherwise there'll be carryover from one step to another. The topmost edge of the sample may suffer from incomplete polymerization using UV, but that bit could be considered sacrificial ... it won't be pretty when sectioned anyway.
If you don't have the foam/sponge, wadded-up lens paper should work.
Phil ------------- Philip Oshel Imaging Facility Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 989 774-3576 office 989 774-7567 lab
-----Original Message----- From: "microscopy.listserver-at-gmail.com" {microscopy.listserver-at-gmail.com} Reply-To: "microscopy.listserver-at-gmail.com" {microscopy.listserver-at-gmail.com} Date: Thursday, 15August, 2019 at 19:14 To: "Oshel, Philip Eugene" {oshel1pe-at-cmich.edu} Subject: [Microscopy] Freeze substitution of ice cream
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X-from: Ning, Gang {gxn7-at-psu.edu}
Hi all,
I received some ice cream samples for freeze substitution and lowicryl HM20 embedding. My problem is the samples are floating on the surface of substitution cocktail, do not sink into the bottom of the vial. Does anybody have experience with this kind of experiment? How can I keep samples submerged in the solution and embedding medium to get good substitution and then UV polymerization?
Thanks for the input. Yes, we do have a good cryoSEM setup and have done ice cream with it in other projects. However, for this specific experiment, we want to know the TEM structure of the ice cream and immunolabeling to identify specific components in it, maybe do CLEM.
---------------------------------------------------------------------------------------------------- *From:* microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} *Sent:* Monday, August 19, 2019 8:28 AM *To:* Ning, Gang {gxn7-at-psu.edu} *Subject:* [Microscopy] Fwd: Freeze substitution of ice cream
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Hi Greg, I am not at all sure whether FS + Embedding in a resin makes any sense with ice cream samples, at all. I would go for anything like cryo-fracture (plus some short freeze-etching / gentle freeze-drying at -80/90/100°C in high vacuum??) , and then go to a cryoSEM (cryo-shuttle), or - if available - a final coating with carbon and/or Pt/C (at or below -100°C) , i.e. like freeze-etching any other bio-sample. Exactly like Debby's suggestion ... HTH.
kind regards,
Reinhard ============================
} } } 16.08.2019 um 02:31: } I am not sure if you need SEM or TEM but I have had excellent results with } cryo-SEM and ice cream. } Debby } } Debra Sherman, Founder & CSO } DS Imaging LLC } https://nam01.safelinks.protection.outlook.com/?url=www.dsimagingllc.com&data=02%7C01%7Cgxn7%40psu.edu%7Ce1f15c41e0b148b4ea8708d724a1467c%7C7cf48d453ddb4389a9c1c115526eb52e%7C0%7C0%7C637018147402150951&sdata=GplFTW6%2Bo1j1FoWXPXB%2F%2FppX6O1Afs4nSipn%2BFXECro%3D&reserved=0
} } } On 8/15/19, 7:09 PM, "microscopy.listserver-at-gmail.com" } {microscopy.listserver-at-gmail.com} wrote } ---------------------------------------------------------------------------- }      The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America }      To Subscribe/Unsubscribe -- } https://nam01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.microscopy.com%2FMicroscopyListserver&data=02%7C01%7Cgxn7%40psu.edu%7Ce1f15c41e0b148b4ea8708d724a1467c%7C7cf48d453ddb4389a9c1c115526eb52e%7C0%7C0%7C637018147402150951&sdata=bGx0Zz9BwOUdptSm1lc7nx%2Bi8LuIk9TEssNxq%2Fhhybw%3D&reserved=0
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} } ---------------------------------------------------------------------------- }          X-from:      Ning, Gang {gxn7-at-psu.edu} }          Hi all, }          I received some ice cream samples for freeze substitution and } lowicryl HM20 embedding. My } problem is    the samples are floating on the surface of substitution } cocktail, do not sink into } the bottom of the    vial. Does anybody have experience with this kind of } experiment? How can I } keep samples submerged in    the solution and embedding medium to get good
} substitution and then UV } polymerization? } Â Â Â Â Â Â Â Â Â Thank you! } Â Â Â Â Â Â Â Â Â Greg
-- Prof. Dr. Reinhard Rachel University of Regensburg Centre for EM / Anatomy Faculty of Biology & Preclin. Med. Universitaetsstrasse 31 D-93053 Regensburg - Germany tel +49 941 943 -2837, -1720 mail reinhard.rachel-at-biologie.uni-regensburg.de office: VKL 3.1.29 member of the IFSM board
Next microscopy conferences: - Microscopy Conference MC2019, 1.-5. Sept 2019 in Berlin - EMC2020 in Kopenhagen, 23.-28.8. 2020 (European conference) - MC2021 in Vienna (D-A-CH conference) - next Microbiol. conferences:Â VAAM 8.-11.03. 2020 Leipzig
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Email: erwright2-at-wisc.edu Name: Elizabeth Wright
Message: The Cryo-Electron Microscopy (Cryo-EM) Research Center in the Department of Biochemistry at the University of Wisconsin - Madison, seeks to hire an assistant, associate, or senior scientist as a computer scientist/system administrator. The Center will house a Titan Krios and a Talos Arctica (both instruments equipped with the Falcon III detector, the Gatan Bioquantum K3 energy filter-detector combination, and Volta phase plate system); a Talos 120 kV TEM; and an Aquilos cryo-Dual Beam system. A cryo-CLEM microscope for correlative microscopy pipelines will be available. Ancillary specimen preparation equipment is in place.
As a key member of the Cryo-EM Research Center, the primary responsibilities of the Computer Scientist/System Administrator are to identify Cryo-EM computational/imaging related research problems, provide computational expertise in experimental design and provide computational support for the Cryo-EM Research Center's activities. Working with the Center Director and staff, the Computer Scientist/System Administrator develops and uses existing software, pipelines, and database systems to analyze and manage Cryo-EM structural data. Develops methodologies and provides support associated with the microscope and image detector computers and servers, the Cryo-EM Research Center cluster, and collaborates with other IT units at UW-Madison for cryo-EM structure determination and data interpretation.
The Cryo-EM Center Computer Scientist/System Administrator reports directly to the Cryo-EM Research Center Director.
Apply online at "Jobs at UW" (http://jobs.wisc.edu) under job number 99648. Applications must be received through UW-Madison's online application system.
As part of the online application, and in order to be considered for this position, applicants should upload the following documents: 1) a curriculum vitae (CV) and 2) a brief statement of relevant research experience in cryo-EM. Contact information for three (3) references will be requested at the time of application.
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} } } 20.08.2019 at 14:14: } Thanks for the input. Yes, we do have a good cryoSEM setup and have done ice } cream with it in other } projects. However, for this specific experiment, we want to know the TEM } structure of the ice cream } and immunolabeling to identify specific components in it, maybe do CLEM. } } Best,
Hi Greg, I see your point (although not clearly, yet). I am quite worried to keep the specific structure of ice cream as it is after dehydration, and I do not see a route to keep this structure even by freeze-substitution and embedding. Yes, one drawback for FF: in fractured samples, you get ONE sample (or two samples / replicas). You cannot section / fracture once again. And for CLEM, I do not have a method ... A note: --} Immunolabeling can be done very nicely on fracture replicas. Severs NJ and Robenek H (2008) Freeze-fracture Cytochemistry in Cell Biology. Methods in Cell Biology 88: 181-204 and Kazushi Fujimoto 1995, Freeze-fracture replica electron microscopy ... in J. Cell Science 108, 3443-3449
and I have more literature on this. In fact, the efficiency of labeling on fracture replicas is - often - higher than on sections. good luck! Regards, Reinhard
-- Prof. Dr. Reinhard Rachel University of Regensburg Centre for EM / Anatomy Faculty of Biology & Preclin. Med. Universitaetsstrasse 31 D-93053 Regensburg - Germany tel +49 941 943 -2837, -1720 mail reinhard.rachel-at-biologie.uni-regensburg.de office: VKL 3.1.29 member of the IFSM board
Next microscopy conferences: - Microscopy Conference MC2019, 1.-5. Sept 2019 in Berlin - EMC2020 in Kopenhagen, 23.-28.8. 2020 (European conference) - MC2021 in Vienna (D-A-CH conference) - next Microbiol. conferences: VAAM 8.-11.03. 2020 Leipzig
Argonne National Laboratory is decommissioning a JEOL 100CXII TEM/STEM instrument.
There is a complete gun, column, and associated power supplies, TEM/STEM electronics, a range of pole pieces, specimen holders and manuals. There are NO digital camera's on the instrument. The instrument was bought in the early 1980's, and has not been operated for several years. It was used principally for materials science research.
The microscopes operational condition is unknown, and should NOT be considered resurrectable. If you are in need of spare parts this would be a candidate instrument.
All items are available AS IS and without cost, so long as they are not being acquired for sale. The requester will be responsible for any packing/shipping/transport expenses from the Argonne site, just outside of Chicago, Illinois.
If anyone is in interested in this vintage microscope please contact me by Noon CST, Friday August 23. Starting on Monday August 26, disassembly will begin and any remaining components will sent out for recycling.
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Cheers....
Nestor Your Friendly Neighborhood SysOp
-- =========================================== Dr. Nestor J. Zaluzec Argonne National Laboratory Photon Sciences Division Bldg 212/A-143 Argonne, Illinois 60439 USA
Senior Scientist - Argonne National Laboratory Fellow of the Microscopy Society of America Fellow of the MicroAnalysis Society Senior Institute Fellow - NAISE - Northwestern University Senior Institute Fellow - UChicago CASE E.P. Wigner Fellow - Oak Ridge National Laboratory Past President Microscopy Society of America Adjunct Professor of Physics - Northern Illinois University & the University of Illinois at Chicago Visiting Professor of Microscopy - Manchester University
===========================================
The box said ... "This program requires Win 95/98/NT/2K/XP/Vista or better..." So I bought a Mac !
We have a Hitachi S4300 SE/N FESEM which is now 12 years old. The column and vacuum system are in great condition, with the microscope operating to specification after its latest service.
I am looking at attempting to future proof the instrument and as Hitachi will not be upgrading the interface or software I was wondering if anyone out there has had experience with developing a new interface for a similar microscope or one of the companies that do that?
E colin.veitch-at-csiro.au T +61 3 5246 4891 M 0438 538 475 F +61 3 5246 4057 Address CSIRO Manufacturing, 75 Pigdons Road, Waurn Ponds, Vic 3216, Australia. www.csiro.au | http://www.csiro.au/Organisation-Structure/Flagships/Manufacturing.aspx
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there might be a possibility to either completely refurbish the electronics and / or the image acquisition system with electronics from www.pointelectronic.de
Check at their website if this is a solution for you. There is no partner in Australia but they are doing world-wide service / setup out of Germany.
Stefan
...Just a satisfied customer...
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
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Email: jdsugar-at-sandia.gov Name: Josh Sugar
Organization: Sandia National Laboratories
Title-Subject: [Filtered] Postdoctoral Position in Transmission Electron Microscopy
Message: Sandia CA has an open postdoctoral position for candidates with TEM experience. Please see job id 667944 at the careers.sandia.gov website or the information below to apply.
From devlinrobb1haoem-at-gmail.com Sat Aug 24 07:15:04 2019 Return-Path: {devlinrobb1haoem-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.162.208] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x7OCF3jI002700 for {microscopylistserverarchive7-at-microscopy.com} ; Sat, 24 Aug 2019 07:15:04 -0500 Message-ID: {2FDADE61.053234C5-at-gmail.com}
X-from: Bil Schneider {wfschneider-at-wisc.edu}
We are trying to gauge if there would be enough interest to try and organize a session or gathering for M&M 2020, possibly in the Technologists Forum? The subject matter would be some of the day to day challenges of operating microscopy and microanalysis labs including:
Low tech innovative (as well as high tech) sample preparation Optimizing tool life and functionality, as well as online scheduling programs Useful user training for efficient and safe use of instruments Black boxes are very useful, but … is any answer the correct answer? Vacuum problems The Bottom Line: Beneficial knowledge could be shared to an array of lab operators, users, and supporters.
Feel free to suggest any other areas of interest pertinent to this general focus in your reply.
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Email: pkohli-at-chem.siu.edu Name: Punit Kohli
Organization: Southern Illinois University Carbondale
Title-Subject: [Filtered] Oxford X-Max 50 detector vaccum problem
Message: Hi,
We have an Oxford X-Max 50 EDS detector attached to FEI Quanta 450 in our centralized microscopic facility. The microscope is not holding vacuum (the vacuum is more than 3-4 x 10^-4 torr in the sample chamber). Thermo-Fisher (which now owns FEI) engineer said that there seems to be a vacuum leakage with the Oxford EDS detector. The detector is being cooled, that means the electronics and power-supply are probably working well. I am wondering if someone has seen this in their systems and what they find on this. We are short on resources, and I am wondering if there is a simpler solution to fixing this than sending the system to Oxford Instruments in England for repair (which I believe will be expensive and time consuming). Any suggestions/advice are highly appreciative.
Thanks,
Punit Kohli Professor Department of Chemistry and Biochemistry Southern Illinois University Carbondale, IL 62901 pkohli-at-chem.siu.edu 618-453-2895
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I would start by replacing the detector temporarily with the blank port- that part should be around the tool somewhere. If it will then pump down you know for sure the Oxford leaks. Which is very rare.
Steve
Steve Perry
} On Aug 27, 2019, at 8:08 AM, microscopy.listserver-at-gmail.com wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: pkohli-at-chem.siu.edu } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy to both } pkohli-at-chem.siu.edu, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can } benefit from our collective wisdom } --------------------------------------------------------------------------- } } Email: pkohli-at-chem.siu.edu Name: Punit Kohli } } Organization: Southern Illinois University Carbondale } } Title-Subject: [Filtered] Oxford X-Max 50 detector vaccum problem } } Message: Hi, } } We have an Oxford X-Max 50 EDS detector attached to FEI Quanta 450 in our centralized microscopic } facility. The microscope is not holding vacuum (the vacuum is more than 3-4 x 10^-4 torr in the } sample chamber). Thermo-Fisher (which now owns FEI) engineer said that there seems to be a vacuum } leakage with the Oxford EDS detector. The detector is being cooled, that means the electronics and } power-supply are probably working well. I am wondering if someone has seen this in their systems } and what they find on this. We are short on resources, and I am wondering if there is a simpler } solution to fixing this than sending the system to Oxford Instruments in England for repair (which I } believe will be expensive and time consuming). Any suggestions/advice are highly appreciative. } } Thanks, } } Punit Kohli } Professor } Department of Chemistry and Biochemistry } Southern Illinois University } Carbondale, IL 62901 } pkohli-at-chem.siu.edu } 618-453-2895 } } Login Host: 131.230.108.55 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 12, 53 -- From microscopy.listserver-at-gmail.com Tue Aug 27 07:03:34 2019 } 12, 53 -- Received: from mail-io1-f53.google.com (mail-io1-f53.google.com [209.85.166.53]) } 12, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x7RC3YJj028341 } 12, 53 -- for {microscopy-at-microscopy.com} ; Tue, 27 Aug 2019 07:03:34 -0500 } 12, 53 -- Received: by mail-io1-f53.google.com with SMTP id j5so45649313ioj.8 } 12, 53 -- for {microscopy-at-microscopy.com} ; Tue, 27 Aug 2019 05:07:53 -0700 (PDT) } 12, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 12, 53 -- d=gmail.com; s=20161025; } 12, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 12, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 12, 53 -- bh=mimbXlQWkNXT3KrNF80LxtTO6MSzMGsy+czvhvehTQE=; } 12, 53 -- b=toWBHhCVF0hqVbv0ltQ1nOJNpNNDhNcDxGQhV+TfrAcM6b0GF+rJKRGF+2Px0QZHbF } 12, 53 -- KtfPlICiGNnZYEp7bg9/9HW3sahxAJYpMM5cY7+pkLuNQKHgqzYiZNVx47PyBhw9pRSe } 12, 53 -- +elWrrSO2kTYWNKLqaGlq4DTlVtnzmyQsyPAAhIIL74wTHFXStcTY9cHlMQ7V6ru7VDS } 12, 53 -- GkT3mAlPXt3l6LGHPN+6//HdUzwiZYuLqcmWBZ3gLWXxzX6EliczL2RLuxKQ8zRCt1oB } 12, 53 -- 7us5mk0MfcUjrf0asKdRQsCyUmOTJaN21rLaAHAaUJNsGXfqxpMxDpyTGSBk11hXjjJn } 12, 53 -- eBuw== } 12, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 12, 53 -- d=1e100.net; s=20161025; } 12, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 12, 53 -- :user-agent:mime-version:in-reply-to:content-language } 12, 53 -- :content-transfer-encoding; } 12, 53 -- bh=mimbXlQWkNXT3KrNF80LxtTO6MSzMGsy+czvhvehTQE=; } 12, 53 -- b=g1SpVl+0FUmHJLOtlI2UuTZN/2ZUUZVO63vvmgQ1VhO3R0mEL47CHAxfQ+jJWtjAwA } 12, 53 -- 4hdT93J/uxyO/QbY7HRJRqn+Gh30JEXLOafTLg74PVPjUT4Z78VwTSDdO5+3UVCzxN5f } 12, 53 -- 1LLe7qJzWk6bVkr8D1utn1lp6/yWmzmqJ9q5wTR1YpPMJbBDum6Fm7H8LpNhhrveUUkj } 12, 53 -- hmQ+ZV2Rwx9wdG5PBFXj8BYmkeWqkb5CXH7RpOAb0eStzqh+sbb7caT0R27Nh200sQSy } 12, 53 -- Ff/qEKDwoOae6pUBgoibFWBS1tbbxc6OTmiwWI8Qy8BeQZojDCN/ySMbkbvbMoaYgu6s } 12, 53 -- aXOA== } 12, 53 -- X-Gm-Message-State: APjAAAUiRrzBXF/0HelEwEEyZWRK8zyCcdrJPxRTGnPWs2uUjzMsoEf4 } 12, 53 -- wJLiwN959v5ZSpnbTm/YddN7XaIX } 12, 53 -- X-Google-Smtp-Source: APXvYqxcp557PFQAlaTCuPOD5dkiJOT6zKNNkBsqZ4MliBoNunnWcZPQ1RUUEsyj6kbLca2KGIo5rw== } 12, 53 -- X-Received: by 2002:a5d:8cd2:: with SMTP id k18mr7568059iot.242.1566907673264; } 12, 53 -- Tue, 27 Aug 2019 05:07:53 -0700 (PDT) } 12, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:846c:9c4f:dd56:8583]) } 12, 53 -- by smtp.googlemail.com with ESMTPSA id r2sm12452346ioh.61.2019.08.27.05.07.52 } 12, 53 -- for {microscopy-at-microscopy.com} } 12, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 12, 53 -- Tue, 27 Aug 2019 05:07:52 -0700 (PDT) } 12, 53 -- Subject: viaWWW:Oxford X-Max 50 detector vacuum problem } 12, 53 -- References: {201908262241.x7QMfCUL027602-at-microscopy.com} } 12, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 12, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 12, 53 -- X-Forwarded-Message-Id: {201908262241.x7QMfCUL027602-at-microscopy.com} } 12, 53 -- Message-ID: {1ecefe81-bed4-dd14-de44-8b2d5f2fae2b-at-gmail.com} } 12, 53 -- Date: Tue, 27 Aug 2019 07:07:51 -0500 } 12, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.14; rv:60.0) } 12, 53 -- Gecko/20100101 Thunderbird/60.8.0 } 12, 53 -- MIME-Version: 1.0 } 12, 53 -- In-Reply-To: {201908262241.x7QMfCUL027602-at-microscopy.com} } 12, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 12, 53 -- Content-Language: en-US } 12, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
X-from: Kolmakov, Andrei A. (Fed) {andrei.kolmakov-at-nist.gov} Punit, you need to do a leak test. The leak can be somewhere else as well, not necessarily at EDS detector. The simplest test is to spray IPA at potential leaky connections while monitoring the vacuum gage. The pressure reduction or increase will indicate the faulty gasket. I remember Aldo used to have He leak detector. This is the best option. Andrei
-----Original Message----- X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Tuesday, August 27, 2019 8:11 AM To: Kolmakov, Andrei A. (Fed) {andrei.kolmakov-at-nist.gov}
X-from: Michael Brazil {mbrazil-at-vergason.com}
I have been working with Doug Connors of TNAS. They repair older EDS detectors. There are now conducting a free diagnostic on mine. http://www.tnas.net/edxproducts.html
Michael Brazil Senior Scientist Vergason Technology, Inc. 166 Route 224 Van Etten, NY 14889 (607) 589-3914 (office) (607) 227-1868 (mobile) mbrazil-at-vergason.com
www.vergason.com www.linkedin.com/in/mbrazil/en
-----Original Message----- X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Tuesday, August 27, 2019 8:10 AM To: Michael Brazil {mbrazil-at-vergason.com}
X-from: pkohli-at-chem.siu.edu
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Email: pkohli-at-chem.siu.edu Name: Punit Kohli
Organization: Southern Illinois University Carbondale
Title-Subject: [Filtered] Oxford X-Max 50 detector vaccum problem
Message: Hi,
We have an Oxford X-Max 50 EDS detector attached to FEI Quanta 450 in our centralized microscopic facility. The microscope is not holding vacuum (the vacuum is more than 3-4 x 10^-4 torr in the sample chamber). Thermo-Fisher (which now owns FEI) engineer said that there seems to be a vacuum leakage with the Oxford EDS detector. The detector is being cooled, that means the electronics and power-supply are probably working well. I am wondering if someone has seen this in their systems and what they find on this. We are short on resources, and I am wondering if there is a simpler solution to fixing this than sending the system to Oxford Instruments in England for repair (which I believe will be expensive and time consuming). Any suggestions/advice are highly appreciative.
Thanks,
Punit Kohli Professor Department of Chemistry and Biochemistry Southern Illinois University Carbondale, IL 62901 pkohli-at-chem.siu.edu 618-453-2895
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I believe there is an o-ring at the interface of the Oxford EDS detector to the SEM chamber. If it was confirmed that the leak came from around the EDS detector, it might be something to look into.
Regards, Kyle Tsai
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X-from: Kolmakov, Andrei A. (Fed) {andrei.kolmakov-at-nist.gov} Punit, you need to do a leak test. The leak can be somewhere else as well, not necessarily at EDS detector. The simplest test is to spray IPA at potential leaky connections while monitoring the vacuum gage. The pressure reduction or increase will indicate the faulty gasket. I remember Aldo used to have He leak detector. This is the best option. Andrei
-----Original Message----- X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Tuesday, August 27, 2019 8:11 AM To: Kolmakov, Andrei A. (Fed) {andrei.kolmakov-at-nist.gov}
X-from: jhyun-at-gatan.com
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Email: jhyun-at-gatan.com Name: John Hyun
Organization: Gatan
Title-Subject: [Filtered] Used 300 kV Schottky (S)TEM wanted
Message: Fellow Microscopists,
We are looking for a used, 300 kV Schottky (S)TEM for use at our R&D headquarters in Pleasanton, CA. Minimum requirements are:
1) 300 kV (required) 2) Schottky emitter (required) 3) Located in North America (required) 4) Side entry stage (required) 5) STEM unit (required) 6) Service supported by original manufacturer (required) 7) Operation can be verified and witnessed by Gatan (required) 8) Fits in ordinary lab space {4m x 5m x 4.5m (L x W x H)} (required) 9) { 15 years old (required) 10) { 10 years old (preferred) 11) 5 mm or larger objective lens gap (preferred)
Gatan is looking to trade new, current generation Gatan equipment for this used TEM. The Gatan equipment received in trade must be installed on an existing TEM/SEM (not a newly purchased instrument). Please contact info-at-gatan.com if you are interested in the opportunity. Thank you.
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X-from: Ferenc Molnar {ferenc.l.molnar-at-googlemail.com}
Dear everybody,
actually to replace the EDS with blind flange seems to be the best idea to verify leaking of it. If the EDS is leaking we may have two options: (1) O-Ring between flange and SEM chamber has an issue (broken, dirty) } you can verify this with the IPA-approach (spray it around the flange and monitor vacuum level). This can be applied also to other suspicious parts for leakage... (2) 2nd option of failure is a broken EDS window, and sorry to say, you will realize this very shortly in complete failure of EDS... If the window is broken you have to send it in for repair - no other option :(
Am Di., 27. Aug. 2019 um 15:13Â Uhr schrieb {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } :
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X-from: Steve Perry {steve.sem-at-icloud.com {mailto:steve.sem-at-icloud.com} }
I would start by replacing the detector temporarily with the blank port- that part should be around the tool somewhere. If it will then pump down you know for sure the Oxford leaks. Which is very rare.
Steve
Steve Perry
} On Aug 27, 2019, at 8:08 AM, microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: pkohli-at-chem.siu.edu {mailto:pkohli-at-chem.siu.edu} } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy to both } pkohli-at-chem.siu.edu {mailto:pkohli-at-chem.siu.edu} , Microscopy-at-Microscopy.com so that  all Microscopy Listserver Subscribers can } benefit from our collective wisdom } --------------------------------------------------------------------------- } } Email: pkohli-at-chem.siu.edu {mailto:pkohli-at-chem.siu.edu} Name: Punit Kohli } } Organization: Southern Illinois University Carbondale } } Title-Subject: [Filtered] Oxford X-Max 50 detector vaccum problem } } Message: Hi, } } We have an Oxford X-Max 50 EDS detector attached to FEI Quanta 450 in our centralized microscopic } facility. The microscope is not holding vacuum (the vacuum is more than 3-4 x 10^-4 torr in the } sample chamber). Thermo-Fisher (which now owns FEI) engineer said that there seems to be a vacuum } leakage with the Oxford EDS detector. The detector is being cooled, that means the electronics and } power-supply are probably working well. I am wondering if someone has seen this in their systems } and what they find on this. We are short on resources, and I am wondering if there is a simpler } solution to fixing this than sending the system to Oxford Instruments in England for repair (which I } believe will be expensive and time consuming). Any suggestions/advice are highly appreciative. } } Thanks, } } Punit Kohli } Professor } Department of Chemistry and Biochemistry } Southern Illinois University } Carbondale, IL 62901 } pkohli-at-chem.siu.edu {mailto:pkohli-at-chem.siu.edu} } 618-453-2895 } }  Login Host: 131.230.108.55 }  Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 12, 53 -- From microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} Tue Aug 27 07:03:34 2019 } 12, 53 -- Received: from mail-io1-f53.google.com {http://mail-io1-f53.google.com} (mail-io1-f53.google.com {http://mail-io1-f53.google.com} [209.85.166.53]) } 12, 53 --  by microscopy.com {http://microscopy.com} (8.12.11.20060308/8.12.8) with ESMTP id x7RC3YJj028341 } 12, 53 --  for {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } ; 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-- Ferenc Molnar Diplomphysiker / Physicist Schwetzingen, Deutschland / Germany
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Email: joseph.uknalis-at-usda.gov Name: Joe Uknalis
Title-Subject: [Filtered] Job Hazard analysis for EM labs
Message: Hiya, could anyone share their Job Hazard Analysis sheets for various EM lab procedures?
specifically: SEM/EDX TEM specimen prep embedding staining Critical point dryer (specifically Denton CPD-1)
Hate to recreate the wheel if I don't have to!
thanks
Joe
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Email: martis-at-stanford.edu Name: Joel Martis
Organization: Stanford University
Title-Subject: [Filtered] Looking for a FEI Cryo transfer holder
Message: Hello,
Hello, I am a graduate student at Stanford working on Cryo-EM. We are looking for a used FEI cryo transfer holder. Any leads are appreciated!
Thanks, Joel
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From aaronlittle895pudju-at-gmail.com Sun Sep 1 02:22:11 2019 Return-Path: {aaronlittle895pudju-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.162.198] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x817MApW016409 for {microscopylistserverarchive7-at-microscopy.com} ; Sun, 1 Sep 2019 02:22:11 -0500 Received: from [184.79.40.5] by qrx.quickslick.com with ASMTP; Sun, 01 Sep 2019 10:15:54 +0300 Message-ID: {E536279E.55751F64-at-gmail.com}
We will (presumably) be moving into a new building next year here at the University of Hawaii and have decided not to take our 1985 Balzers 400T freeze-fracture freeze-etch unit. I am willing to pull out parts and send them to anyone for the cost of shipping. If you want the whole thing or larger parts, you might have to come to pack it up yourself. I have the accompanying books and schematics. I spent a fair amount of time a few years ago getting the pumping system working again, but at that time the electrodes did not want to fire. Turbo pump was replaced sometime in the eighties. I have some of the small prep parts. I believe it?s 220V 3-phase (one reason it?s not going with us). Interested? Questions? For faster response use tinacarv-at-hawaii.edu
Aloha, Tina
*************************************************************************** Tina (Weatherby) Carvalho tinacarv-at-hawaii.edu Biological Electron Microscope Facility (808) 956-6251 University of Hawaii at Manoa http://www.pbrc.hawaii.edu/bemf ***************************************************************************
==============================Original Headers============================== 3, 20 -- From tina-at-pbrc.hawaii.edu Tue Sep 3 20:58:02 2019 3, 20 -- Received: from b1000.pbrc.hawaii.edu (b1000.pbrc.hawaii.edu [128.171.22.30]) 3, 20 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x841w1XG024802 3, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 3 Sep 2019 20:58:01 -0500 3, 20 -- Received: from b1000.pbrc.hawaii.edu (localhost [127.0.0.1]) 3, 20 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id x8422h6G000723 3, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 3, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 3 Sep 2019 16:02:43 -1000 (HST) 3, 20 -- Received: from localhost (tina-at-localhost) 3, 20 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP id x8422gGf000719 3, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 3 Sep 2019 16:02:43 -1000 (HST) 3, 20 -- X-Authentication-Warning: b1000.pbrc.hawaii.edu: tina owned process doing -bs 3, 20 -- Date: Tue, 3 Sep 2019 16:02:42 -1000 (HST) 3, 20 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 3, 20 -- X-X-Sender: tina-at-b1000 3, 20 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 3, 20 -- Subject: Balzers 400T Freeze-fracture Freeze-etch unit up for grabs or parts 3, 20 -- Message-ID: {Pine.GSO.4.64.1909031601210.699-at-b1000} 3, 20 -- MIME-Version: 1.0 3, 20 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
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Title-Subject: [Filtered] Which Model of Spinning Disk microscope is better in the field/market now?
Message: Dear All, We are planning to get a new spinning disk microscope for our BioImaging facility. Can anyone suggest us Which Model of Spinning Disk microscope is better in the field/market now? 1. Yokogawa Spinning Disk Field Scanning Confocal System CSU-W1 or CSU-X1? 2. What is your Openion abour SoRa - Super Resolution unit 3. what about Andor's Dragonfly super resolution unit?
Please give your opinions and suggestions, that will help us to take a decision. Thank You in advance..
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X-from: Helen Turner {helen.turner-at-waikato.ac.nz}
*ABSTRACT SUBMISSION DEADLINE EXTENDED TO SEPTEMBER 20th* *EARLY BIRD REGISTRATIONS DEADLINE SEPTEMBER 27th*
The 29^th New Zealand Conference on Microscopy will be held at the University of Waikato from Monday 11^th to Thursday 14^th of November 2019. The conference will cover all forms of microscopy in all disciplines and so offers something for everyone. We have three internationally renowned speakers:
Prof Juliet Gerrard, Chief Science Adviser to the NZ Prime Minister and biochemist, University of Auckland. Prof Jason Swedlow, University of Dundee, cell biologist and OME (Open Microscopy Environment) co-founder. Prof Raynald Gauvin, McGill University, Metallurgist and CASINO program creator.
There will be workshops on Advanced Fluorescence, EBSD, OME (workflows in OMERO) and Image Analysis and Machine Learning. As well as a host of prizes and awards available we have a full social programme.
Please take the time to look at the website: microscopy2019.co.nz {http://microscopy2019.co.nz}
Kind regards,
Helen.
-- Helen M Turner BSc(Hons) Electron Microscope Facility Faculty of Science and Engineering The University of Waikato Private Bag 3105 Hamilton 3240 New Zealand
From kipemilf56vpilo-at-gmail.com Wed Sep 4 12:36:23 2019 Return-Path: {kipemilf56vpilo-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.162.204] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x84HaLQg004951 for {microscopylistserverarchive7-at-microscopy.com} ; Wed, 4 Sep 2019 12:36:22 -0500 Message-ID: {B024D57B.6002E6A6-at-gmail.com}
Join us for the webinar series on X-ray Computed Tomography for Materials Science to find out how X-ray CT can help your research.
When: September 25, 2019 11:00 AM Pacific Time Part 3: X-ray Computed Tomography for Materials Science: Food and Pharmaceutical Applications
In this webinar, you will learn: - Keys to soft material imaging - Food science applications - Pharmaceutical applications
The webinar series will cover image analysis and a number of application examples of food, pharmaceutical, composite materials etc.
To learn more, visit: https://www.rigaku.com/en/webinars/x-ray_ct_introduction
Recording of the webinar will be available for registered attendees.
Hope to see you there.
Aya Takase . Senior Scientist Rigaku Americas Corporation 9009 New Trails Drive . The Woodlands, TX 77381 USA T: 281-362-2300 ex 208 . F: 281-364-3628
Aya Takase . Senior Scientist Rigaku Americas Corporation 9009 New Trails Drive . The Woodlands, TX 77381 USA T: 281-362-2300 ex 208 . F: 281-364-3628 Privacy . Terms . Disclaimer Join our webinar series "X-ray CT for Materials Science"
==============================Original Headers============================== 21, 54 -- From btv1==150a303cb14==Aya.Takase-at-rigaku.com Wed Sep 4 14:04:13 2019 21, 54 -- Received: from spamfilter.rigaku.com (spamfilter.rigaku.com [146.20.167.112]) 21, 54 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x84J4Da1009272 21, 54 -- for {Microscopy-at-microscopy.com} ; Wed, 4 Sep 2019 14:04:13 -0500 21, 54 -- X-ASG-Debug-ID: 1567624139-0fdc5125982e8e100001-1DjkGe 21, 54 -- Received: from 592194-Exch1.rac.local ([172.16.230.4]) by spamfilter.rigaku.com with ESMTP id Q9EhGP9974HhmCMO (version=TLSv1.2 cipher=ECDHE-RSA-AES256-SHA384 bits=256 verify=NO) for {Microscopy-at-microscopy.com} ; Wed, 04 Sep 2019 15:08:59 -0400 (EDT) 21, 54 -- X-Barracuda-Envelope-From: Aya.Takase-at-rigaku.com 21, 54 -- X-Barracuda-RBL-Trusted-Forwarder: 172.16.230.4 21, 54 -- Content-Language: en-US 21, 54 -- Content-Type: text/plain; charset="iso-8859-1" 21, 54 -- DKIM-Signature: v=1; a=rsa-sha1; d=rigaku.com; s=dkim1; c=relaxed/relaxed; 21, 54 -- t=1567620533; h=from:subject:to:date:ad-hoc; 21, 54 -- bh=Sbat0hmbT6jbWmr5zXoKWnpACEc=; 21, 54 -- b=kIfUkUtePVknqOh4/eHWKfmDd3tWx/upVJU44J16+eS837q6DpCwL1LM6xzuzVPPy4ju7Z2vYMU 21, 54 -- c7Pi/kMovDnrdghtiAklkBGaE3wgPcJ3Fdb4W1IA0zZfLDIxB/VXfrgxYKWeUF5zDsbVqW8UJAJvx 21, 54 -- jk4H+JdvQWjZyv14hbZR0O5uGhqqug6p6lxAi7xD+y4tk4sICfoSHnqgK/geQExNy5vvGoQz1mlhA 21, 54 -- 9kVxa/IU2ogLeIbZYf8l0S29IuY5PHOPBWHY8HwLYJV6dmR+EIvu9kLEj8yY2+HccEyzL/Bq/WDNr 21, 54 -- nbNTgKiqDXUqkJf/diIxzN6nXTBvBonyHRcw== 21, 54 -- Received: from 592200-EXCH2.rac.local (172.16.230.5) by 592194-Exch1.rac.local 21, 54 -- (172.16.230.4) with Microsoft SMTP Server (TLS) id 15.0.1365.1; Wed, 4 Sep 21, 54 -- 2019 14:08:52 -0400 21, 54 -- Received: from 592200-EXCH2.rac.local ([fe80::821:2890:77ec:aa4f]) by 21, 54 -- 592200-Exch2.rac.local ([fe80::821:2890:77ec:aa4f%16]) with mapi id 21, 54 -- 15.00.1365.000; Wed, 4 Sep 2019 14:08:52 -0400 21, 54 -- X-Barracuda-RBL-Trusted-Forwarder: 172.16.230.4 21, 54 -- From: Aya Takase {Aya.Takase-at-rigaku.com} 21, 54 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 21, 54 -- Subject: Webinar series: X-ray Computed Tomography for Materials Science 21, 54 -- Thread-Topic: Webinar series: X-ray Computed Tomography for Materials Science 21, 54 -- X-ASG-Orig-Subj: Webinar series: X-ray Computed Tomography for Materials Science 21, 54 -- Thread-Index: AdViZ29AnEctX+0sQBiz3ELo8+S+FQ== 21, 54 -- Date: Wed, 4 Sep 2019 18:08:51 +0000 21, 54 -- Message-ID: {9482bc838e2342a9b0498357d8e61dd9-at-592200-Exch2.rac.local} 21, 54 -- Accept-Language: en-US 21, 54 -- X-MS-Has-Attach: 21, 54 -- X-MS-TNEF-Correlator: 21, 54 -- x-ms-exchange-transport-fromentityheader: Hosted 21, 54 -- x-originating-ip: [10.2.2.190] 21, 54 -- MIME-Version: 1.0 21, 54 -- X-Barracuda-Connect: UNKNOWN[172.16.230.4] 21, 54 -- X-Barracuda-Start-Time: 1567624139 21, 54 -- X-Barracuda-Encrypted: ECDHE-RSA-AES256-SHA384 21, 54 -- X-Barracuda-URL: https://spamfilter.rigaku.com:443/cgi-mod/mark.cgi 21, 54 -- X-Virus-Scanned: by bsmtpd at rigaku.com 21, 54 -- X-Barracuda-Scan-Msg-Size: 1164 21, 54 -- X-Barracuda-BRTS-Status: 1 21, 54 -- X-Barracuda-Spam-Score: 0.00 21, 54 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using global scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=5.0 KILL_LEVEL=1000.0 tests= 21, 54 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.76066 21, 54 -- Rule breakdown below 21, 54 -- pts rule name description 21, 54 -- ---- ---------------------- -------------------------------------------------- 21, 54 -- Content-Transfer-Encoding: 8bit 21, 54 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id x84J4Da1009272 ==============================End of - Headers==============================
From husechristena45quaey-at-gmail.com Fri Sep 6 17:03:06 2019 Return-Path: {husechristena45quaey-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.162.192] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x86M34UV011017 for {microscopylistserverarchive7-at-microscopy.com} ; Fri, 6 Sep 2019 17:03:05 -0500 Received: from unknown (HELO smtp.doneohx.com) (Fri, 06 Sep 2019 13:58:25 -0800) by qnx.mdrost.com with SMTP; Fri, 06 Sep 2019 13:58:25 -0800 Received: from unknown (HELO mx03.listsystemsf.net) (Fri, 06 Sep 2019 13:46:13 -0800) by rly04.hottestmile.com with LOCAL; Fri, 06 Sep 2019 13:46:13 -0800 Received: from rsmail.alkoholic.net ([81.242.130.238]) by external.newsubdomain.com with QMQP; Fri, 06 Sep 2019 13:33:05 -0800 Message-ID: {C7EE5F18.B3217CA7-at-gmail.com}
X-from: Ning, Gang {gxn7-at-psu.edu}
Dear all,
An old vacuum oven in my lab is finally broken after 20+ maybe 30 years in service. I am looking for a replacement in a reasonable price. We only use it for polymerization of TEM samples, have never used vacuum function. Any input and suggestion is welcome and highly appreciated.
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Email: csown42-at-gmail.com Name: Christopher Own
Title-Subject: [Filtered] Looking for quantities of spent AEI filaments
Message: Hi fellow microscopists!
I am looking to obtain quantities of spent AEI filaments. The status of the filament wire is not important, I am just looking for the ceramic carriers. If you or your facility have any spent filaments or boxes, and are looking to make space please let me know!
Thank you,
Dr. Chris Own
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I’m currently trying to do TEM-EDX on chloroplasts to detect the presence of CdTe. The CdTe is not in high density, and the presence of other metals in the sample from general fixatives/stains like osmium, uranium, lead, even potassium from the buffer, is confounding our search as they form nanoparticles that accumulate throughout the sample. I was wondering if anyone could suggest fixatives/buffers/stains that lack metals to replace any of these steps? Currently planning on trying tannic acid without uranyl acetate to substitute osmium. Thank you in advance,
Matt Dickson Staff Microscopist Central Facility for Advanced Microscopy and Microanalysis University of California, Riverside
Does anybody by chance have a TEM with an additional electron or ion beam?
All the best,
Philip
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Hello Matt, Also look into potassium permanganate as an osmium substitute. Good Luck, Michael Delannoy
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Friday, September 13, 2019 1:13 AM To: Michael Delannoy
X-from: matthew.dickson.417-at-my.csun.edu
Hello everyone,
I’m currently trying to do TEM-EDX on chloroplasts to detect the presence of CdTe. The CdTe is not in high density, and the presence of other metals in the sample from general fixatives/stains like osmium, uranium, lead, even potassium from the buffer, is confounding our search as they form nanoparticles that accumulate throughout the sample. I was wondering if anyone could suggest fixatives/buffers/stains that lack metals to replace any of these steps? Currently planning on trying tannic acid without uranyl acetate to substitute osmium. Thank you in advance,
Matt Dickson Staff Microscopist Central Facility for Advanced Microscopy and Microanalysis University of California, Riverside
From hennjohn6cyees-at-gmail.com Fri Sep 13 09:20:37 2019 Return-Path: {hennjohn6cyees-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.162.202] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x8DEKYuM015131 for {microscopylistserverarchive7-at-microscopy.com} ; Fri, 13 Sep 2019 09:20:35 -0500 Message-ID: {070B2FA1.1F1013AD-at-gmail.com}
X-from: Peter Ingram {peter.ingram-at-duke.edu}
For a start you should be using STEM/EDX mapping rather than TEM alone…….
…maybe you are + cryo techniques.
Good luck!
Peter Ingram Sr. Physicist Adj. Professor of Pathology Duke University Medical Center PO Box 90319 DURHAM NC 27708-0319
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Hi Matt, You could use optical dyes to take optical images of your TEM section, then use correlation microscopy to identify areas of interest in your EM images (which will be mostly gray everywhere). In this way you don't need to use heavy metal staining. If you need to identify very fine features, you could use Au immunolabeling, the Au particles should be easily distinguishable from the CdTe. Regards Stefano
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X-from: matthew.dickson.417-at-my.csun.edu
Hello everyone,
I’m currently trying to do TEM-EDX on chloroplasts to detect the presence of CdTe. The CdTe is not in high density, and the presence of other metals in the sample from general fixatives/stains like osmium, uranium, lead, even potassium from the buffer, is confounding our search as they form nanoparticles that accumulate throughout the sample. I was wondering if anyone could suggest fixatives/buffers/stains that lack metals to replace any of these steps? Currently planning on trying tannic acid without uranyl acetate to substitute osmium. Thank you in advance,
Matt Dickson Staff Microscopist Central Facility for Advanced Microscopy and Microanalysis University of California, Riverside
by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x8DJdoZ8006200 for {MicroscopyListserverArchive7-at-microscopy.com} ; Fri, 13 Sep 2019 14:39:50 -0500 Received: (from mail-at-localhost) by microscopy.com (8.12.11.20060308/8.12.11/Submit) id x8DJdlIg006196; Fri, 13 Sep 2019 14:39:47 -0500
X-from: Erico Freitas {ericotadeu-at-ufmg.br}
Dear Matt Dickson,
If the CdTe your trying to detect are nanoparticles (NPs), why don't you try to perform dark field imaging of the chloroplasts. You should be able to see the CdTe NPs distribution on chloroplasts then, and don't need to worry about the fixatives. I have done that for Au NPs on vesicles. Another possibility is to perform ADF-STEM or (better HAADF-STEM) imaging of the chloroplasts and play with the camera length to enhance the contrast of CdTe NPs. Both dark field (TEM and STEM) modes will be much faster then EDX mapping. And once you have seen the CdTe NPs on chloroplasts, then you can try EDX to complement your data.
Best regards,
Em sex, 13 de set de 2019 Ă s 02:17, {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } escreveu:
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I’m currently trying to do TEM-EDX on chloroplasts to detect the presence of CdTe. The CdTe is not in high density, and the presence of other metals in the sample from general fixatives/stains like osmium, uranium, lead, even potassium from the buffer, is confounding our search as they form nanoparticles that accumulate throughout the sample. I was wondering if anyone could suggest fixatives/buffers/stains that lack metals to replace any of these steps? Currently planning on trying tannic acid without uranyl acetate to substitute osmium. Thank you in advance,
Matt Dickson Staff Microscopist Central Facility for Advanced Microscopy and Microanalysis University of California, Riverside
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-- Erico Freitas
Physicist/Microscopist at Center of Microscopy Universidade Federal de Minas Gerais (UFMG) Av. Antônio Carlos, 6627, Campus Pampulha, Belo Horizonte, MG, Brazil. ZIP Code 31270-901. +55-31-3409-7573 +55-31-3409-7575
Coordinator:Transmission Electron Microscopy laboratory
} On Sep 12, 2019, at 10:08 PM, microscopy.listserver-at-gmail.com wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } } } X-from: matthew.dickson.417-at-my.csun.edu } } Hello everyone, } } I’m currently trying to do TEM-EDX on chloroplasts to detect the } presence of CdTe. The CdTe is not in high density, and the presence of } other metals in the sample from general fixatives/stains like osmium, } uranium, lead, even potassium from the buffer, is confounding our search } as they form nanoparticles that accumulate throughout the sample. I was } wondering if anyone could suggest fixatives/buffers/stains that lack } metals to replace any of these steps? } Currently planning on trying tannic acid without uranyl acetate to } substitute osmium. } Thank you in advance, } } Matt Dickson } Staff Microscopist } Central Facility for Advanced Microscopy and Microanalysis University of } California, Riverside } Hi Matt, You might try an ammonium buffer to avoid potassium. Of course, not all specimens are happy in this buffer, so YMMV. Yours, Bill
} On Sep 12, 2019, at 10:08 PM, microscopy.listserver-at-gmail.com wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } } } X-from: matthew.dickson.417-at-my.csun.edu } } Hello everyone, } } I’m currently trying to do TEM-EDX on chloroplasts to detect the } presence of CdTe. The CdTe is not in high density, and the presence of } other metals in the sample from general fixatives/stains like osmium, } uranium, lead, even potassium from the buffer, is confounding our search } as they form nanoparticles that accumulate throughout the sample. I was } wondering if anyone could suggest fixatives/buffers/stains that lack } metals to replace any of these steps? } Currently planning on trying tannic acid without uranyl acetate to } substitute osmium. } Thank you in advance, } } Matt Dickson } Staff Microscopist } Central Facility for Advanced Microscopy and Microanalysis University of } California, Riverside } Hi Matt, You might try an ammonium buffer to avoid potassium. Of course, not all specimens are happy in this buffer, so YMMV. Yours, Bill
I would like to bring your attention to a newly posted position of senior research associate at Yale Medical School. Yale university is investing and expanding in biological electron microscopy, to that end we are seeking to hire an experienced electron microscopist to join our team at Center for Cellular and Molecular Imaging (CCMI).
The main job responsibilities are listed here, to learn more details regarding this position or to apply, please go to: https://sjobs.brassring.com/TGnewUI/Search/Home/Home?partnerid=25053&siteid=5248#home, and enter the requisition number "58001BR".
- Perform various tasks in biological electron microscopy, such as sample preparation and sectioning, imaging with transmission and scanning electron microscopes for ultrastructure (cell, tissue), immune-labeling and negative staining. - Assist users with the EM experiments and microscope viewing and imaging. - Maintain and troubleshoot TEM, SEM and sample preparation instrument. - Train students or postdocs on independent operation of electron microscopes and ancillary equipment. - Develop projects of correlative light and electron microscopy (CLEM). - Able to learn and perform advanced microscopy for example, electron tomography, Focus-Ion-Beam SEM (FIB-SEM) 3D volume imaging, high pressure freezing and cryo ultramicrotome sectioning. - Assist the facility director with additional tasks (grant and publication support, classes/workshops, etc.).
Please feel free to contact me if you have any questions.
Sincerely,
Xinran Nick Liu, M.D. & Ph.D. Director, Center for Cellular & Molecular Imaging Bio & Cryo Electron Microscopy Core Facilities Yale University School of Medicine Office Phone: 203.785.4050 Lab Phone: 203.785.5390 http://medicine.yale.edu/ccmi/em
Argonne National Laboratory has had simultaneous "Multi-Beam" (electron + ion) TEM's since the late 1970's. Originally this was on a 1.2 MeV HVEM and then the facility slowly migrated to a 300 kV TEM in the 90's after the HVEM was decommissioned.
This is a national user facilities which was originally operated by the former Electron Microscopy Center at ANL, and is now run the by the Nuclear Science Division of ANL for in-situ irradiation damage studies in materials.
Proposals are required and ciritically reviewed for relevance and programmatic need, upon acceptance time is allocated free of charge.
More details about the ANL IVEM-Tandem can be found at this URL.
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Email: ralvaradojr-at-ufl.edu Name: Rudy Alvarado
Organization: University of Florida - ICBR EM Core
Message: Hi, Has anyone have issues infiltration/embedding tissue (less than 1 mm^2) with HM20 resin? I've been having issues with chatter using HM20 resin (new batch) and areas in tissue not infiltrating well. I first tried an old batch of HM20 resin, and then a new batch of HM20 resin. Both ended up with white, crusty tissue embedded in the resin (mostly the top and middle part of the tissue). After milling until all of the crusty part was gone and the tissue seems ok to section, there was too much chatter. I would get no improvement after curing it further in -20 deg C with UV light for 24 hours +. Sample: liver tissue Process: immunoelectron microscopy
Thank you, Rudy
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-------- Forwarded Message -------- X-from: Regan M {reganhll-at-gmail.com}
Hi ,
I think infiltration is not complete due to incomplete dehydration, I would suggest you to increase the time for dehydration in alcohol for higher times to enhance infiltration. If you could share your embedding protocol, I could suggest you further steps,
greetings,
Regan.
On Tue, Sep 17, 2019 at 12:30 AM {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote:
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Message: Hi, Has anyone have issues infiltration/embedding tissue (less than 1 mm^2) with HM20 resin? I've been having issues with chatter using HM20 resin (new batch) and areas in tissue not infiltrating well. I first tried an old batch of HM20 resin, and then a new batch of HM20 resin. Both ended up with white, crusty tissue embedded in the resin (mostly the top and middle part of the tissue). After milling until all of the crusty part was gone and the tissue seems ok to section, there was too much chatter. I would get no improvement after curing it further in -20 deg C with UV light for 24 hours +. Sample: liver tissue Process: immunoelectron microscopy
Thank you, Rudy
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From ketcdutj170dkiss-at-gmail.com Tue Sep 17 00:09:04 2019 Return-Path: {ketcdutj170dkiss-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.162.197] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x8H592Cf023641 for {microscopylistserverarchive7-at-microscopy.com} ; Tue, 17 Sep 2019 00:09:03 -0500 Message-ID: {593411B9.A391257E-at-gmail.com}
X-from: ralvaradojr-at-ufl.edu
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Email: ralvaradojr-at-ufl.edu Name: Rudy Alvarado
Organization: University of Florida
Title-Subject: [Filtered] cont. of HM20 resin issue...HELP! Message: Hi, To answer your questions, this is what I have done.
Trial 1 - room tempertature - immunoelectron microscopy processing protocol: Liver pieces were fixed in 4% paraformaldehyde and 0.5% gluteraldehyde in PBS (pH: 7.20). They were washed in PBS, water, and dehydrated in short graded ethanol series (25, 50, 75, 95, 100). Tissue were washed in anhydrous ethanol and infilterated in 50% HM20 resin with Z6040 embedding primer, followed by 100% resin exchanges with Z6040 (left it infiltrating overnight and and did 4 exchanges every hour or so). I then cured the tissue in -20 deg C with UV light with fresh HM20 resin with Z6040 for 24 hours. HM20: mixed for 2 hours with N(g) bubbling I also used a microwave processor during the processing.
Trial 2 - I did a long graded ethanol dehydration series (25, 50, 55, 65, 75, 80, 85, 90, 95, 100) followed by anhydrous ethanol washes. I also increase the HM20 resin exchanges to three days (2 overnights with a total of 6 resin exchanges). Everything else is the same as trial 1. Trial 3 (in the process) - High pressure frozen tissue pieces in 1-hexadecene as my cryo-protectant. HPF samples were placed in immunoelectron microscopy cocktail (0.5% gluteraldehyde, 0.1% UA in acetone) and left in LN temp dewar until free substitution. Hope this helps to address my issue with HM20 resin. Best, Rudy
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Title-Subject: [Filtered] computer simulation software for TEM
Message: Does anyone know how to get the JEMS software from Pierre Stadelmann? The link at http://www.jems-saas.ch/ gives an error and when I send email to Pierre's email address I also get an error that the email address is not found.
Thanks,
Lourdes Login Host: 128.8.138.214 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
The following URL currently works for JEMS downloads
https://www.jems-swiss.ch
the Email address is:
jems.swiss-at-gmail.com
Cheers,
Nestor Your Friendly Neighborhood SysOp
On 9/17/19 12:25 PM, riba-at-umd.edu wrote: } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy to both riba-at-umd.edu, Microscopy-at-Microscopy.com so that } all Microscopy Listserver Subscribers can benefit from our collective wisdom } --------------------------------------------------------------------------- } } Email: riba-at-umd.edu } Name: Lourdes Guadalupe Salamanca-Riba } } Organization: University of Maryland } } Title-Subject: [Filtered] computer simulation software for TEM } } Message: Does anyone know how to get the JEMS software from Pierre Stadelmann? The link at http://www.jems-saas.ch/ gives an error and when I send email to Pierre's email address I also get an error that the email address is not found. } } Thanks, } } Lourdes } } Login Host: 128.8.138.214 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } }
Hello everyone, I had written that I was having a problem with my CM12 TEM in that when I lifted the viewing screen to fast, it would shut the scope off. Later we noticed a small spark that would jump from the plate to the metal housing of the vacuum chamber.
TSS solved the problem. It appears there is a circuit connected to the view screen powdered by two batteries. One of the batteries was dead. When that was replaced and problem disappeared.
I know, not a very interesting note, can't really call it a story but it appears I am wrong.
I just got an e-mail from someone who wants to publish a book (no, really!) based on my screen lifter problem. They claim they will pay all the publishing cost.
I see it now: the hero is a hard working electron named Icey Electron called on a hero's journey in which he picks up a rag tag band of friends and companions to battle the evil villain Electro-Arc. Too bad I trashed the note....
Stay safe...........
Frank Karl Microscopist Akron Rubber Development Laboratory 2887 Gilchrist Road Akron, Ohio 44305
We've been discussing this at several occasions, and it is said (by Heinz Schwartz among others) that especially the HM20 monostep can be difficult due to partly polymerization prior to use, and that some brand new bottles in fact are not OK.
But of cause also do as mentioned in another post, look at the dehydration.
Best regards, Randi Olsen
-----Original Message----- X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: 17. september 2019 00:26 To: Randi Olsen {randi.olsen-at-uit.no}
X-from: ralvaradojr-at-ufl.edu
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Email: ralvaradojr-at-ufl.edu Name: Rudy Alvarado
Organization: University of Florida - ICBR EM Core
Message: Hi, Has anyone have issues infiltration/embedding tissue (less than 1 mm^2) with HM20 resin? I've been having issues with chatter using HM20 resin (new batch) and areas in tissue not infiltrating well. I first tried an old batch of HM20 resin, and then a new batch of HM20 resin. Both ended up with white, crusty tissue embedded in the resin (mostly the top and middle part of the tissue). After milling until all of the crusty part was gone and the tissue seems ok to section, there was too much chatter. I would get no improvement after curing it further in -20 deg C with UV light for 24 hours +. Sample: liver tissue Process: immunoelectron microscopy
Thank you, Rudy
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The TEM simulation JEMS software fom Pierre stadelmann has recently changed.
Now it is "jems.swiss-at-gmail.com"
Regards
Philippe Buffat
On 18.09.19 14:32, microscopy.listserver-at-gmail.com wrote: } Title-Subject: [Filtered] computer simulation software for TEM } } Message: Does anyone know how to get the JEMS software from Pierre Stadelmann? The link at } http://www.jems-saas.ch/ gives an error and when I send email to Pierre's email address I also get } an error that the email address is not found. } --
Prof. Em. Philippe A. Buffat Ecole Polytechnique Federale de Lausanne
Centre Interdisciplinaire de Microscopie Electronique
Station 12, 1015 Lausanne, Switzerland (http://cime.epfl.ch {http://cime.epfl.ch/} , https://people.epfl.ch/philippe.buffat?lang=en)
Chemin des Vioz 14, CH-1865 Les Diablerets, Switzerland mobile +41 79 337 9384 philippe.buffat-at-epfl.ch {mailto:philippe.buffat-at-epfl.ch}
Hello, Can you tell us the source of your HM20? Sincerely, Michael Delannoy
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Wednesday, September 18, 2019 8:44 AM To: Michael Delannoy
X-from: Randi Olsen {randi.olsen-at-uit.no}
Hi,
We've been discussing this at several occasions, and it is said (by Heinz Schwartz among others) that especially the HM20 monostep can be difficult due to partly polymerization prior to use, and that some brand new bottles in fact are not OK.
But of cause also do as mentioned in another post, look at the dehydration.
Best regards, Randi Olsen
-----Original Message----- X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: 17. september 2019 00:26 To: Randi Olsen {randi.olsen-at-uit.no}
X-from: ralvaradojr-at-ufl.edu
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Email: ralvaradojr-at-ufl.edu Name: Rudy Alvarado
Organization: University of Florida - ICBR EM Core
Message: Hi, Has anyone have issues infiltration/embedding tissue (less than 1 mm^2) with HM20 resin? I've been having issues with chatter using HM20 resin (new batch) and areas in tissue not infiltrating well. I first tried an old batch of HM20 resin, and then a new batch of HM20 resin. Both ended up with white, crusty tissue embedded in the resin (mostly the top and middle part of the tissue). After milling until all of the crusty part was gone and the tissue seems ok to section, there was too much chatter. I would get no improvement after curing it further in -20 deg C with UV light for 24 hours +. Sample: liver tissue Process: immunoelectron microscopy
Thank you, Rudy
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I would think bad polymerization may be the issue -
It says in EMS's HM20 technical data sheet -since the initiation of the polymerization is largely independent of temperature, blocks may be polymerized at the same temperatures which are used for infiltration. I wonder whether you infiltrated your samples at -20C for the last few steps before curing them at -20C.
Another thing may be the embedding molds. I usually use BEEM, sometimes gelatin capsules. I experienced bad polymerization with flat embedding molds.
I've never used Z-6040. How does it work? Can it have some bad effect?
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X-from: Randi Olsen {randi.olsen-at-uit.no}
Hi,
We've been discussing this at several occasions, and it is said (by Heinz Schwartz among others) that especially the HM20 monostep can be difficult due to partly polymerization prior to use, and that some brand new bottles in fact are not OK.
But of cause also do as mentioned in another post, look at the dehydration.
Best regards, Randi Olsen
-----Original Message----- X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: 17. september 2019 00:26 To: Randi Olsen {randi.olsen-at-uit.no}
X-from: Regan M {reganhll-at-gmail.com}
Thanks for detail protocol.
The second protocol looks good, I could see problems with 1st protocol. If in case the 3rd protocol does not work, I suggest starting infiltration with  20% HM20 resin, gradually move to 100% with 2 days of time at 4 degrees to preserve immunogenicity.
Also keep a control just pure resign without any tissue sample to check the performance of resign, sometimes the resin absorbs moisture if the bottle is left open, and makes it difficult to polymerise.
greetings, Regan.
On Wed, Sep 18, 2019 at 2:36 PM {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote:
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Title-Subject: [Filtered] cont. of HM20 resin issue...HELP! Message: Hi, To answer your questions, this is what I have done.
Trial 1 - room tempertature - immunoelectron microscopy processing protocol: Liver pieces were fixed in 4% paraformaldehyde and 0.5% gluteraldehyde in PBS (pH: 7.20). They were washed in PBS, water, and dehydrated in short graded ethanol series (25, 50, 75, 95, 100). Tissue were washed in anhydrous ethanol and infilterated in 50% HM20 resin with Z6040 embedding primer, followed by 100% resin exchanges with Z6040 (left it infiltrating overnight and and did 4 exchanges every hour or so). I then cured the tissue in -20 deg C with UV light with fresh HM20 resin with Z6040 for 24 hours. HM20: mixed for 2 hours with N(g) bubbling I also used a microwave processor during the processing.
Trial 2 - I did a long graded ethanol dehydration series (25, 50, 55, 65, 75, 80, 85, 90, 95, 100) followed by anhydrous ethanol washes. I also increase the HM20 resin exchanges to three days (2 overnights with a total of 6 resin exchanges). Everything else is the same as trial 1. Trial 3 (in the process) - High pressure frozen tissue pieces in 1-hexadecene as my cryo-protectant. HPF samples were placed in immunoelectron microscopy cocktail (0.5% gluteraldehyde, 0.1% UA in acetone) and left in LN temp dewar until free substitution. Hope this helps to address my issue with HM20 resin. Best, Rudy
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-----Original Message----- X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: 19. september 2019 02:54 To: Randi Olsen {randi.olsen-at-uit.no}
X-from: Michael Delannoy {mdelann1-at-jhmi.edu}
Hello, Can you tell us the source of your HM20? Sincerely, Michael Delannoy
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Wednesday, September 18, 2019 8:44 AM To: Michael Delannoy
X-from: Randi Olsen {randi.olsen-at-uit.no}
Hi,
We've been discussing this at several occasions, and it is said (by Heinz Schwartz among others) that especially the HM20 monostep can be difficult due to partly polymerization prior to use, and that some brand new bottles in fact are not OK.
But of cause also do as mentioned in another post, look at the dehydration.
Best regards, Randi Olsen
-----Original Message----- X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: 17. september 2019 00:26 To: Randi Olsen {randi.olsen-at-uit.no}
X-from: ralvaradojr-at-ufl.edu
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Email: ralvaradojr-at-ufl.edu Name: Rudy Alvarado
Organization: University of Florida - ICBR EM Core
Message: Hi, Has anyone have issues infiltration/embedding tissue (less than 1 mm^2) with HM20 resin? I've been having issues with chatter using HM20 resin (new batch) and areas in tissue not infiltrating well. I first tried an old batch of HM20 resin, and then a new batch of HM20 resin. Both ended up with white, crusty tissue embedded in the resin (mostly the top and middle part of the tissue). After milling until all of the crusty part was gone and the tissue seems ok to section, there was too much chatter. I would get no improvement after curing it further in -20 deg C with UV light for 24 hours +. Sample: liver tissue Process: immunoelectron microscopy
Thank you, Rudy
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X-from: ralvaradojr-at-ufl.edu
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Email: ralvaradojr-at-ufl.edu Name: Rudy Alvarado
Organization: University of Florida - ICBR EM Core
Title-Subject: [Filtered] cont. HM20 resin issue
Message: We get the lowicryl HM20 kit from EMS. As for infiltration, I do my infiltration exchanges with the aid of the microwave (3 mins -at- 120 W with 20 mmHg vacuum pressure) followed by 30-60 minutes on the orbital rocker at 4 deg C. On the last infiltration exchange, I let it sit at -20 deg C for 60-120 minutes before UV curing it at -20 deg C in the free substitution unit. I think it might be the flat BEEM capsules we are using. So on this HPF/FS samples, I will try both flat BEEM capsules and gelatin capsules. Z-6040 embedding primer (from EMS) supposed to help with sample and resin adhesion, and prevent sample from pulling away from resin when sectioning.I don't know the chemistry behind it though. Lastly, I never had bad effects from using it.
- Rudy Login Host: 128.227.204.60 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
I'm a little astonished with how much Voodoo comes up in this HM20 thread. HM20 is really not complicated to use. 1. Make sure you don’t bubble air through the resin. Mixing the components carefully by pipetting without bubbling is enough. It's stable in the fridge/freezer for at least a month. 2. Polymerize the resin under nitrogen gas (=in the AFS) if you have open/leaky vials. 3. If you can't polymerize under nitrogen, make sure your vials are filled to the top with resin and seal them as well as you can. 4. Polymerize 24-48 h under UV, if it didn’t polymerize by then it will never polymerize (The only thing that will happen is that the unpolymerized resin will slowly evaporate). Let sit open, overnight in the hood for any unpolymerized resin to evaporate. This should give you dry, hard blocks. They can be soft at the top if they were in contact with air.
If you have infiltration problems (which is most likely the case based on your description), do more and longer dehydration/infiltration steps. There is a slight viscosity difference between the solvent and HM20, which in dense tissues can cause poor infiltration. If you start infiltration with 25% HM20 in solvent that problem should go away. Also keep in mind that 1-hexadecene has a melting point of 4 C, it will be solid in the FS. If you sample is surrounded by it, the excess solvent in the FS will dissolve it. If it's deep in your tissue (= you threw the tissue into hexadecene and let it sit) it's not going to come out at -20 C.
Z-6040 is a silane (dive into the MSDS [3-(2,3-epoxypropoxy)propyl]trimethoxysilane ). I struggle to understand why you would want to use that, particularly for HM20. The common use is for materials science samples where you have a flat surface of some solid material that doesn’t bond with the resin, such as metal foils. The silane covers the inert surface and has an epoxy group that can now crosslink with an epoxy resin mixture.
I'm also not a fan of applying a vacuum to resins because you're not entirely sure what resin components evaporate and what stays behind.
Good luck! Chris
-----Original Message----- X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Friday, September 20, 2019 10:11 AM To: c.buser-at-oak-crest.org
X-from: ralvaradojr-at-ufl.edu
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Email: ralvaradojr-at-ufl.edu Name: Rudy Alvarado
Organization: University of Florida - ICBR EM Core
Title-Subject: [Filtered] cont. HM20 resin issue
Message: We get the lowicryl HM20 kit from EMS. As for infiltration, I do my infiltration exchanges with the aid of the microwave (3 mins -at- 120 W with 20 mmHg vacuum pressure) followed by 30-60 minutes on the orbital rocker at 4 deg C. On the last infiltration exchange, I let it sit at -20 deg C for 60-120 minutes before UV curing it at -20 deg C in the free substitution unit. I think it might be the flat BEEM capsules we are using. So on this HPF/FS samples, I will try both flat BEEM capsules and gelatin capsules. Z-6040 embedding primer (from EMS) supposed to help with sample and resin adhesion, and prevent sample from pulling away from resin when sectioning.I don't know the chemistry behind it though. Lastly, I never had bad effects from using it.
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Email: ravi.thakkar369-at-gmail.com Name: Ravi
Title-Subject: [Filtered] Remove knife marks,chattering bands from TEM image.
Message: Hi, Does any one help me remove knife marks and chattering effects from TEM image?
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I didn't see a list reply yet but figured I would include the list in case someone wants to check my logic here. So a disclaimer first, as my first MS mentor said "Data is data, images are data, images are made up of pixels, thus pixels are data". You must have a very good, sound reasoning behind making some change that fundamentally changed the data as presented. The original image untouched as taken by the CCD/PMT should be made available upon request and all steps taken to modify the image. That being said:
1. Knife marks. For purely artist reasoning (I might have had a ESEM of the fine feathers on the back of a mosquito printed and posted over my bed until the wife threatened it was me or the mosquito image.....), the knife mark is a little difficult to get rid of. Assuming that the image lost by the mark is mostly uniform to the immediate region. I never played with imagej to this effect, and dislike photoshop. GIMP however does an excellent job at doing touch-ups, what you would want to search for is fixing a scratch in a photograph. It should get you somewhere close. For data analysis, I'd avoid this sort of manipulation at all costs. It really becomes a question of does it effect the area of interest.
2. Chatter. Assuming this is fairly uniform, to some degree FFT does an okay job. Again would stress not using this for data analysis. In imagej you would do a FFT, delete the area of repeated signal, and inverse FFT back. There are a few guides online for doing this, and think imagej is better suited. I've done this for electrical noise or ground vibration, unsure exactly how well it would handle real world chatter. The fall back would be doing a similar touch-up as i suggested for a knife mark but this is a lot of manual manipulation that can't easily apply a algorithm to (ie FFT, delete this region, iFFT back).
But ultimately, both of these things are mostly avoidable. To some degree, the knife mark sometimes you just have to life with, and people would map this diamonds to know what's a good region for higher quality or important cuts. Then just hope it didn't go through a vital spot you needed to image. Chatter, there are a dozen ways to help work on that. Change speed, knife angle, hardness of embedding media, total size of face being cut, section thickness, knife sharpness (glass), etc. You really do want to work on removing these issues physically prior to post processing manipulations if at all possible.
Just my 2 cents, but it's been maybe 15 years since I've touched an ultramicrotome.
Regards -Jason
On Sat, Sep 21, 2019 at 6:57 PM {microscopy.listserver-at-gmail.com} wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: ravi.thakkar369-at-gmail.com } } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy to both } ravi.thakkar369-at-gmail.com, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers } can benefit from our collective wisdom } --------------------------------------------------------------------------- } } Email: ravi.thakkar369-at-gmail.com Name: Ravi } } Title-Subject: [Filtered] Remove knife marks,chattering bands from TEM image. } } Message: Hi, } Does any one help me remove knife marks and chattering effects from TEM image? 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From smithdiana157xecu-at-gmail.com Mon Sep 23 09:35:14 2019 Return-Path: {smithdiana157xecu-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.162.198] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x8NEZDui024777 for {microscopylistserverarchive7-at-microscopy.com} ; Mon, 23 Sep 2019 09:35:14 -0500 Received: from unknown (HELO mtu67.syds.piswix.net) (Mon, 23 Sep 2019 04:40:23 -1000) by m1.gns.snv.thisdomainl.com with ASMTP; Mon, 23 Sep 2019 04:40:23 -1000 Message-ID: {5B8214D4.F50FB3FC-at-gmail.com}
Dear All,
anybody out there who has the electronic schematics of the HHV (Edwards) ScanCoat Six sputtercoater?
I need to repair one and would at least have some information...
Thanks,
Stefan
-- ----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
==============================Original Headers============================== 9, 22 -- From stefan.diller-at-t-online.de Wed Sep 25 10:07:14 2019 9, 22 -- Received: from mailout07.t-online.de (mailout07.t-online.de [194.25.134.83]) 9, 22 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x8PF7Cv8012043 9, 22 -- for {microscopy-at-microscopy.com} ; Wed, 25 Sep 2019 10:07:12 -0500 9, 22 -- Received: from fwd10.aul.t-online.de (fwd10.aul.t-online.de [172.20.26.152]) 9, 22 -- by mailout07.t-online.de (Postfix) with SMTP id EE9B54271914 9, 22 -- for {microscopy-at-microscopy.com} ; Wed, 25 Sep 2019 17:13:06 +0200 (CEST) 9, 22 -- Received: from [192.168.2.115] (V+GJGuZBZhWFGj1dqkgSxx6X03GImaeaotvVSNOOuuche7+aCFJovJGOpxKqQutQzk-at-[31.16.250.216]) by fwd10.t-online.de 9, 22 -- with (TLSv1.2:ECDHE-RSA-AES256-GCM-SHA384 encrypted) 9, 22 -- esmtp id 1iD8yY-46QdcW0; Wed, 25 Sep 2019 17:13:06 +0200 9, 22 -- To: microscopy-at-microscopy.com 9, 22 -- From: Stefan Diller {stefan.diller-at-t-online.de} 9, 22 -- Subject: HHV (Edwards) ScanCoat Six manual and schematics 9, 22 -- Message-ID: {2d52e21e-a523-4d99-67f1-1223640793e3-at-t-online.de} 9, 22 -- Date: Wed, 25 Sep 2019 17:12:33 +0200 9, 22 -- User-Agent: Mozilla/5.0 (Windows NT 10.0; WOW64; rv:68.0) Gecko/20100101 9, 22 -- Thunderbird/68.1.0 9, 22 -- MIME-Version: 1.0 9, 22 -- Content-Type: text/plain; charset=utf-8; format=flowed 9, 22 -- Content-Transfer-Encoding: 7bit 9, 22 -- X-ID: V+GJGuZBZhWFGj1dqkgSxx6X03GImaeaotvVSNOOuuche7+aCFJovJGOpxKqQutQzk 9, 22 -- X-TOI-MSGID: 64ab20f5-18f6-4409-8dd2-164640597ca2 ==============================End of - Headers==============================
From fritschemichael755bxiue-at-gmail.com Thu Sep 26 16:40:57 2019 Return-Path: {fritschemichael755bxiue-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.162.209] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x8QLetqx017476 for {microscopylistserverarchive7-at-microscopy.com} ; Thu, 26 Sep 2019 16:40:56 -0500 Received: from mail.naihautsui.co.kr ([192.147.36.167]) by mxs.perenter.com with LOCAL; Thu, 26 Sep 2019 21:31:00 +0000 Received: from mx03.listsystemsf.net ([Thu, 26 Sep 2019 21:18:53 +0000]) by rsmail.alkoholic.net with LOCAL; Thu, 26 Sep 2019 21:18:53 +0000 Received: from [19.172.121.81] by qrx.quickslick.com with ESMTP; Thu, 26 Sep 2019 21:16:12 +0000 Received: from webmail.halftomorrow.com [130.16.157.130] by smtp.mixedthings.net with NNFMP; Thu, 26 Sep 2019 21:12:42 +0000 Message-ID: {DC1CA6B9.9C18962D-at-gmail.com}
Dear colleagues
The YouTube channel "M*N: Microscopy, Machine Learning, Materials" dedicated to the applications of big data, machine learning, and artificial intelligence in Scanning Transmission Electron Microscopy and Scanning Probe Microscopy is now fully updated with the historical overview lectures. These include:
1. Unsupervised learning in Scanning Probe Microscopy: Spectroscopies 2. Supervised learning in Scanning Probe Microscopy: Spectroscopies 3. Linear unmixing: basic techniques and some applications in microscopy and spectroscopy 4. Supervised and unsupervised learning in Scanning Probe Microscopy: Imaging 5. Supervised and Unsupervised Learning in Scanning Transmission Electron Microscopy 6. Learning Physics (and Chemistry) from Scanning Transmission Electron Microscopy 7. Atomic fabrication by STEM: feedback, compressed sensing, and non-rectangular beam paths
The channel now also features the "Z Corner" list started Maxim Ziatdinov, containing the short tutorial lectures on recent developments on ML in STEM, including: 1. Jupyter papers: scientific papers with data and code 2. Introduction to deep learning with PyTorch in Google Colab 3. How to work with AICrystallographer in Google Colab The new lectures are becoming available once the associated Jupyter notebooks are developed.
These lectures are closely tied to the on-line data and code resources. The codes are (or will be) shared via the PyCroscopy domain on the GitHub. https://github.com/pycroscopy/pyCroscopy
Finally, the imaging tools can accessed via the Center for Nanophase Materials Sciences. https://www.ornl.gov/facility/cnms
We are delighted to host our second annual International Symposium on Advanced Microscopy and Spectroscopy (ISAMS-2). The symposium will focus on the retrieval of hidden structural, chemical, electronic, orbital and spin information at the sub-atomic scale by leveraging the novel instruments that can capture electron scattering, and energy loss processes at unprecedented time and energy scales. Topical subjects of in situ microscopy such as gas and liquid cells, electrical and mechanical manipulation, and radiation damage will also be discussed.
Along with the ISAMS-2, IMRI is pleased to host the first UC Irvine School on Transmission Electron Microscopy (UCI-STEM) after the symposium. The primary focus of the school this year will be on liquid cell TEM & STEM and spectroscopy (two separate tracks). The school will emphasize both theoretical background and practical aspects of instrument operation. We encourage anyone who would like to increase knowledge and skills on liquid cell TEM, STEM and spectroscopy techniques to participate in our first UC Irvine Microscopy School.
University of California, Irvine ISAMS-2: December 8-10, 2019 UCI-STEM: December 11-13, 2019 https://sites.uci.edu/isams2/
Irvine Materials Research Institute (IMRI) is a newly established interdisciplinary organization under the Office of Research of the University of California, Irvine (UCI). It serves as the cross-campus nexus for materials research at UCI. IMRI operates a wide range of state-of-the-art, open-access user facilities for the characterization of materials, biological samples and devices from sub-A to macroscopic length scales - available to all university, industry and non-profit researchers. It offers advanced techniques and services with professional staff support.
Conference Chair Xiaoqing Pan
Program Committee Will Bowman, Shane Gonen, Xiaoqing Pan, Joe Patterson, and Huolin Xin
Organization Committee Toshihiro Aoki, Xiaofeng Liu, Shelly Nazarenus, Li Xing, Mingjie Xu, and Jian-Guo Zheng Coordinator, Mary Ang
Venues University of California, Irvine CALIT2 Building Auditorium
Abstract Submission Email to: isams-at-uci.edu; Submission Deadline: October 15, 2019
From duranelizabethe2arer-at-gmail.com Sat Sep 28 01:13:02 2019 Return-Path: {duranelizabethe2arer-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.162.197] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x8S6D0lt013616 for {microscopylistserverarchive7-at-microscopy.com} ; Sat, 28 Sep 2019 01:13:01 -0500 Message-ID: {88535A68.36009B01-at-gmail.com}
Dear All,
as a substitute for a problematic operation panel at a high-school SEM I am looking for a free but still operable Jeol JSM 840 in Germany.
Any help is welcome.
Thanks,
Stefan
--
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
==============================Original Headers============================== 11, 23 -- From stefan.diller-at-t-online.de Mon Sep 30 02:54:41 2019 11, 23 -- Received: from mailout04.t-online.de (mailout04.t-online.de [194.25.134.18]) 11, 23 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x8U7sf1F010581 11, 23 -- for {microscopy-at-microscopy.com} ; Mon, 30 Sep 2019 02:54:41 -0500 11, 23 -- Received: from fwd11.aul.t-online.de (fwd11.aul.t-online.de [172.20.27.152]) 11, 23 -- by mailout04.t-online.de (Postfix) with SMTP id 436D441A837A 11, 23 -- for {microscopy-at-microscopy.com} ; Mon, 30 Sep 2019 10:00:51 +0200 (CEST) 11, 23 -- Received: from mac-pro.local (VgQ8K4ZeZhCAbP5IN3KhrYCOsXils+GKaVgnsJXW3L05l+ZSFdDsEtrHw6JthFjgdD-at-[31.16.250.216]) by fwd11.t-online.de 11, 23 -- with (TLSv1.2:ECDHE-RSA-AES256-GCM-SHA384 encrypted) 11, 23 -- esmtp id 1iEqbu-4geQ2y0; Mon, 30 Sep 2019 10:00:46 +0200 11, 23 -- To: "Microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 11, 23 -- From: Stefan Diller {stefan.diller-at-t-online.de} 11, 23 -- Subject: Looking for operation panel Jeol JSM840 in Germany 11, 23 -- Message-ID: {57d002c4-a062-1247-dbe8-5d2344fef36a-at-t-online.de} 11, 23 -- Date: Mon, 30 Sep 2019 10:00:46 +0200 11, 23 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:60.0) 11, 23 -- Gecko/20100101 Thunderbird/60.9.0 11, 23 -- MIME-Version: 1.0 11, 23 -- Content-Type: text/plain; charset=windows-1252; format=flowed 11, 23 -- Content-Transfer-Encoding: 7bit 11, 23 -- Content-Language: en-GB 11, 23 -- X-ID: VgQ8K4ZeZhCAbP5IN3KhrYCOsXils+GKaVgnsJXW3L05l+ZSFdDsEtrHw6JthFjgdD 11, 23 -- X-TOI-MSGID: 4ac1eaed-6c59-483e-ba63-fe871ece381d ==============================End of - Headers==============================
From jacqalon41migxy-at-gmail.com Tue Oct 1 05:13:52 2019 Return-Path: {jacqalon41migxy-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.162.201] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x91ADpH9022242 for {microscopylistserverarchive7-at-microscopy.com} ; Tue, 1 Oct 2019 05:13:51 -0500 Message-ID: {eb2d01d57807$1fe98700$73dec2cb-at-jacqalon41migxy} Reply-To: "Waylon" {jacqalon41migxy-at-gmail.com}
X-from: stefan.geimer-at-uni-bayreuth.de
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Email: stefan.geimer-at-uni-bayreuth.de Name: Stefan Geimer
Organization: University of Bayreuth
Title-Subject: [Filtered] Anti-HA tag antibody for immunogold / recommendation
Message: Dear list members,
which (commercially available) anti-HA tag antibody (preferably from rabbit) can you recommend for immunogold-experiments (postembedding, LR-Gold)?
Thanks a lot for help,
Stefan
++++++++++++++++++++++++++++++++++++++++++ Stefan Geimer, Ph.D. Universitaet Bayreuth Biologie/Elektronenmikroskopie NW I / B1 Universitaetsstr. 30 95447 Bayreuth Germany ++++++++++++++++++++++++++++++++++++++++++
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both william.a.mark-at-gsk.com, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Email: william.a.mark-at-gsk.com Name: William Mark
Title-Subject: [Filtered] Information on Tiyoda microscopes
Message: I am in possession of several Tiyoda microscope frames of various designs as well as some accessories. I am looking for anyone who may have knowledge or any information on the company and the microscopes they manufactured? There was a Japanese site years ago, but that is no longer active. Any help or guidance is appreciated. These scopes are well made with a high degree of craftsmanship. I would like to somehow preserve them or make a public record of them so having some background info would be helpful.
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*Registration is Now _OPEN_ for our January Facilities Meetings!*
/Free for academics to attend and covering LM, EM and Flow, find out more: /
*UK LM Facilities Meeting 2020*
*Birmingham*
*6-7 January 2020*
**
This event regularly attracts around 100 facility managers and colleagues from all over the country and provides an opportunity for imaging managers to interact, discuss issues and share ideas.
The meeting is informal and free discussion is encouraged. Participation from commercial organisations, microscope manufacturers and software developers is welcomed. The event is an ideal opportunity to exchange feedback and to hear about new technologies.
Registration to the 2 day meeting is *FREE* for academic attendees.
The costs of running this meeting and your attendance is paid for by the generous support of the commercial representatives attending the meeting.
To register for this meeting, please visit www.rms.org.uk/uklmfmm/ {http://www.rms.org.uk/uklmfmm/} .
Event Contact: Kat Driscoll (katdriscoll-at-rms.org.uk {mailto:katdriscoll-at-rms.org.uk} )
This event will attract facility managers and colleagues from all over the country providing an opportunity to interact, discuss issues and share ideas. The meeting is informal and free discussion is encouraged. Participation from commercial organisations, microscope manufacturers and software developers is welcomed. The event is an ideal opportunity to exchange feedback and to hear about new technologies. The meeting will be taking place at the Lindisfarne room, Hadrian’s Building, King’s Road, Newcastle Upon Tyne. This meeting follows on from the successful inaugural meeting that took place in York in January 2018.
Registration to the 2 day meeting is *FREE* for academic attendees. This includes attendance to both days of the meeting, refreshments and lunches and the dinner on Wednesday 8 January. To register for this event, please visit www.rms.org.uk/flow-facilities-meeting {http://www.rms.org.uk/flow-facilities-meeting} . If you are unable to attend the dinner, but have registered for the event, please can you let me know.
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Event Contact: Kate Wooding (kate-at-rms.org.uk {mailto:kate-at-rms.org.uk} )
The EM-UK community meetings are designed to be an open forum for discussion of the latest developments and challenges in the field, suitable for both academic and commercial microscopists. The meeting will include Techno Bites, talks, discussions about training, an evening meal and plenty of networking opportunities.
For 2020 the EM-UK meeting is moving to the conference facilities at the University of Plymouth, located on the South West Coast.
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The meeting is scheduled to start at 12:30pm on Monday 9 January and finishes after lunch on Tuesday 10 January. The meeting is held over 2 days and is *FREE* for academics to attend. The costs of running this meeting and your attendance is paid for by the generous support of the commercial representatives attending the meeting. If you would like to register to attend, please do so on the website, www.rms.org.uk/em-uk-2020/ {http://www.rms.org.uk/em-uk-2020/} . All refreshments, lunch and a dinner on the Monday night are included in your registration.
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*European Microscopy Congress 2020 (emc2020) *
Conference 23 – 28 August 2020 at the The Bella Center, Denmark
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X-from: Helen Turner {helen.turner-at-waikato.ac.nz}
Hi All,
The deadline for abstract submissions for the 29th New Zealand Conference on Microscopy is rapidly approaching - *Friday 27th September*! So please don't delay and submit your abstract today!!
*MNZ2019* will be held at the *University of Waikato* from Monday 11^th  to Thursday 14^th  of November 2019. The conference will cover all forms of microscopy in all disciplines and so offers something for everyone. We have three internationally renowned speakers:
*Prof Juliet Gerrard*, Chief Science Adviser to the NZ Prime Minister and biochemist, University of Auckland. *Prof Jason Swedlow*, University of Dundee, cell biologist and OME (Open Microscopy Environment) co-founder. *Prof Raynald Gauvin*, McGill University, Metallurgist and CASINO program creator.
There will be workshops on *Advanced Fluorescence*, *EBSD*, *OME* (workflows in *OMERO*) and *Image Analysis and Machine Learning*. As well as a host of prizes and awards available we have a full social programme.
Please take the time to look at the website: microscopy2019.co.nz {http://microscopy2019.co.nz/}
Kind regards,
Helen.
-- Helen M Turner  BSc(Hons) Electron Microscope Facility Faculty of Science and Engineering The University of Waikato Private Bag 3105 Hamilton 3240 New Zealand
The Campus Microscopy and Imaging Facility at the Ohio State University is seeking an electron microscopist with expertise in biological specimen preparation for observation with either a TEM or an SEM. Please visit the following link for more information or to apply: https://www.jobsatosu.com/postings/98536.
Sara
Sara Cole, PhD Associate Director Campus Microscopy and Imaging Facility (CMIF) (614) 292-9786 : cole.553-at-osu.edu : cmif.osu.edu
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Hi all - I'm having problems with our Quanta 200 - the interface freezes so often that the instrument is basically not usable.
From the FEI TAD diagnostics I see a problem when the DSPB board (or anything) tries to connect to the "TVLB" - I know what the DSPB board is, but cannot find any mention of what "TVLB" means - does this ring a bell for anyone?
Hi Mark, Have you tried the old defunct website URL you mentioned on the archive.org {http://archive.org} wayback machine? Best wishes, -Nathan
On Thu, Oct 3, 2019, 2:37 AM {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote:
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Email: william.a.mark-at-gsk.com {mailto:william.a.mark-at-gsk.com} Name: William Mark
Title-Subject: [Filtered] Information on Tiyoda microscopes
Message: I am in possession of several Tiyoda microscope frames of various designs as well as some accessories. I am looking for anyone who may have knowledge or any information on the company and the microscopes they manufactured? There was a Japanese site years ago, but that is no longer active. Any help or guidance is appreciated. These scopes are well made with a high degree of craftsmanship. I would like to somehow preserve them or make a public record of them so having some background info would be helpful.
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X-from: Erna van Wilpe {erna.vanwilpe-at-up.ac.za}
Dear Helen
I just want to find out if the New Zealand Conference on Microscopy is held annually or every 2 years?
Best regards, Erna
On Wed, 2 Oct 2019 at 14:49, {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote:
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X-from:Â Â Â Â Â Helen Turner {helen.turner-at-waikato.ac.nz {mailto:helen.turner-at-waikato.ac.nz} }
Hi All,
The deadline for abstract submissions for the 29th New Zealand Conference on Microscopy is rapidly approaching - *Friday 27th September*! So please don't delay and submit your abstract today!!
*MNZ2019* will be held at the *University of Waikato* from Monday 11^th  to Thursday 14^th  of November 2019. The conference will cover all forms of microscopy in all disciplines and so offers something for everyone. We have three internationally renowned speakers:
*Prof Juliet Gerrard*, Chief Science Adviser to the NZ Prime Minister and biochemist, University of Auckland. *Prof Jason Swedlow*, University of Dundee, cell biologist and OME (Open Microscopy Environment) co-founder. *Prof Raynald Gauvin*, McGill University, Metallurgist and CASINO program creator.
There will be workshops on *Advanced Fluorescence*, *EBSD*, *OME* (workflows in *OMERO*) and *Image Analysis and Machine Learning*. As well as a host of prizes and awards available we have a full social programme.
Please take the time to look at the website: microscopy2019.co.nz {http://microscopy2019.co.nz} {http://microscopy2019.co.nz/}
Kind regards,
Helen.
-- Helen M Turner  BSc(Hons) Electron Microscope Facility Faculty of Science and Engineering The University of Waikato Private Bag 3105 Hamilton 3240 New Zealand
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Email: athron-at-ucdavis.edu Name: Andrew Thron
Organization: Materials Science Department / UC Davis
Title-Subject: [Filtered] Aperture Control board for a JEOL 2100F
Message: To the Microscopy Community, The Aperture Control Board on our JEOL 2100F needs to be replaced, and the lead time for a new board is April of next year. Would anyone know where I could find a used Aperture Control board for a JEOL 2100F/2500/2200? Does anyone know of a lab that has to scrap a JEOL 2100F/2500/2200?
Thank you for your Help!
Andrew Thron, Ph. D. Technical Director AMCaT Laboratory University of California 1 Shields Avenue Davis, California 95616-5294 Cell Phone: (916) 220-3786 Lab Phone: (530) 752-0284
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Ken, perhaps the abbreviation „TLVP“ might read as: "Trivial Low Voltage Programmer" (Source: e.g. : http://www.stuartbruce.net/abbrev/4/t.shtml#l) whoever Mr. 'Stuart Bruce' may be....(:-))
But I could guess too: "Treshold Limit Values Protocol"
But you might have received similar answer/results already.
Never made acquaintance with a QUANTA200...., deploring fact.... Best wishes and good luck with your problem….
Regards, Wolfgang
======================================================== MUSS Wolfgang Dr. phil. (PhD) [OR i. R. / en retraite / retired]
FRMS, Retired Member of MSA Scientific Profile at ResearchGate: http://www.researchgate.net/profile/Wolfgang_MUSS Former Head of Electron Microscopy Lab at Institute of Pathology SALK-LKH / Salzburger Landeskliniken | General Hospital and PMU (private) PARACELSUS MEDICAL UNIVERSITY of SALZBURG
Former Secretary and (until June2017) Board Member of the
SCUR {The Society for Cutaneous Ultrastructure Research} „The Skin Imaging Society“ { www.scur.org }
Von: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Gesendet: Freitag, 4. Oktober 2019 14:34 An: wij.muss-at-aon.at Betreff: KEIN SPAM *** SPAM *** [Microscopy] Error with Quanta 200 - "TVLB" error [the interface freezes so often that the instrument is basically not usable]
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Hi all - I'm having problems with our Quanta 200 - the interface freezes so often that the instrument is basically not usable.
From the FEI TAD diagnostics I see a problem when the DSPB board (or anything) tries to connect to the "TVLB" - I know what the DSPB board is, but cannot find any mention of what "TVLB" means - does this ring a bell for anyone?
Regarding my previous answer to Ken and the list I apologize for flooding your mail-boxes with false information by confusing the sought term "TVLB" with "TVLP".
Now that I became aware of that fact I have tried to find a solution for the abbreviation "TVLB" and the only [Google and Yahoo search] trace I was able to find –possibly related with a digital control of function of vacuum parts in a SEM – was:
Ralston QTHA_TVLB In-line Vent Valve https://www.ralstoninst.com/product-datasheet-pdf/Ralston-QTHA-TVLB.pdf Part No. QTHA-TVLB Male Quick-test x male Quick-test in-line vent valve, brass Use to slowly or quickly bleed pressure from a line after a calibration is complete
Specs/Attributes Country of origin: USA Has Block Valve No Has Vent Valve Yes Materials Brass Max Operating Temperature 225 °F / 105 °C Max Pressure 3000 PSI / 200 bar / 20 MPa Max Vacuum 0.00 InHg / 0.0 kPa Min Operating Temperature 0 °F / -18 °C Seal Materials Buna-N, Delrin Dimensions H: 1.5 in (3.81 cm) x W: 2 in (5.08 cm)
https://www.transcat.com/ralston-instruments-qtha-tvlb-qtha-tvlb Connect this valve in between the pressure calibrator and device under test. When testing is complete use the vent valve to bleed pressure from the device under test and none of the process fluid will contaminate the calibrator or hand pump
---------------------------------------------------------------------------- ---------------------------------------------------------------------------- ---------- It could be a hint but if not, I am sorry again. Hopefully somebody knows and is willing to "enlighten the secret"via listserver common reply. ---------------------------------------------------------------------------- ---------------------------------------------------------------------------- ---------- Ken, would you mind to explain your definition of DSPB as mentioned in your initial request? My favorite is 1), second choice: 2). Thank you! DSPB: 1) Data Standards Program Board - 2) Digital Signal Processing Block DSPB: download sect point barrow or Designated Sites Programme Board (:https://www.acronymattic.com/DSPB.html and http://acronymsandslang.com/DSPB-meaning.html) DSP digital signal processing or a Digital Signal Processor (:https://cdiac.ess-dive.lbl.gov/pns/acronyms.html#D. - and http://www.numerix.co.uk/abbrev.html) DSP Directory System Protocol (:http://www.hackeracronyms.com/acroweb.asp?SType=0&Acro=DSP) ------------------------------------- I wish you all a splendid and recreative October-weekend ! Regards,
Wolfgang Muss Retired member of MSA SALZBURG-AUSTRIA
==============================Original Headers============================== 14, 21 -- From wij.muss-at-aon.at Fri Oct 4 11:40:37 2019 14, 21 -- Received: from bsmtp.bon.at (bsmtp.bon.at [213.33.87.14]) 14, 21 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x94GeawV012300 14, 21 -- for {Microscopy-at-Microscopy.com} ; Fri, 4 Oct 2019 11:40:37 -0500 14, 21 -- Received: from MussTHINK (unknown [93.83.25.98]) 14, 21 -- by bsmtp.bon.at (Postfix) with ESMTPSA id 46lG4P2h4Dz5tlH; 14, 21 -- Fri, 4 Oct 2019 18:47:01 +0200 (CEST) 14, 21 -- From: "MUSS Wolfgang Dr. phil./PhD \(OR i.R, retired\)" {wij.muss-at-aon.at} 14, 21 -- To: {Microscopy-at-Microscopy.com} 14, 21 -- Cc: {kpseverin-at-alaska.edu} 14, 21 -- Subject: [Microscopy] RE: Error with Quanta 200 .... Apologies for wrong abbreviation reading 14, 21 -- Date: Fri, 4 Oct 2019 18:47:01 +0200 14, 21 -- Message-ID: {004b01d57ad3$58a00d70$09e02850$-at-aon.at} 14, 21 -- MIME-Version: 1.0 14, 21 -- Content-Type: text/plain; 14, 21 -- charset="iso-8859-1" 14, 21 -- X-Mailer: Microsoft Outlook 15.0 14, 21 -- Thread-Index: AdV60y50D5uMcL9QT3yqMwn/yawbGw== 14, 21 -- Content-Language: de 14, 21 -- Content-Transfer-Encoding: 8bit 14, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id x94GeawV012300 ==============================End of - Headers==============================
From andralbi54iiqi-at-gmail.com Fri Oct 4 13:05:38 2019 Return-Path: {andralbi54iiqi-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.162.197] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x94I5bCg021826 for {microscopylistserverarchive7-at-microscopy.com} ; Fri, 4 Oct 2019 13:05:38 -0500 Message-ID: {00828028.2516E9C8-at-gmail.com}
X-from: lev.klibanov-at-gmail.com
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Email: lev.klibanov-at-gmail.com Name: Lev
Organization: Independent Researcher
Title-Subject: [Filtered] Gatan 600A
Message: Hello All,
We are trying to fix an old Gatan 600A dual mill system and there is some problem with a high voltage.
It would be very helpful if somebody can share the GATAN 600A documentation/electrical schematics.
Please help:)
Thanks in advance.
Lev
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From normanatlas54nekg-at-gmail.com Sun Oct 6 04:25:59 2019 Return-Path: {normanatlas54nekg-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.162.192] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x969PuRQ008806; Sun, 6 Oct 2019 04:25:58 -0500 Received: from unknown (37.33.190.58) by webmail.halftomorrow.com with ESMTP; Sun, 06 Oct 2019 09:19:00 +0000 Message-ID: {2E271103.A9569727-at-gmail.com}
TLDR: do you have one of these? Any known fixes? A spare board perhaps?
BioCurious is trying to use this microscope, but is finding this card makes the control computer unstable. Sometimes BIOS complains about an IRQ conflict, sometimes BIOS goes to a black screen, and sometimes the computer boots. There are a few suspicious things on the PCB, but nothing that we are confident is the issue. If the board is put into another computer, it causes odd behavior ranging from the PCI device not enumerating in Linux to causing the computer itself to not POST.
Some pictures here: https://twitter.com/johndmcmaster/status/1180758413938057223?s=20
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Email: tpepper-at-iastate.edu Name: Tracey Stewart
Organization: Iowa State University
Title-Subject: [Filtered] CryoEM Facility Manager Position at Iowa State
Message: Iowa State University has created a new Cryo-EM Facility and are hiring to fill the position of Facility Manager. The facility is under construction and the new FEI Glacios (with K3 summit detector) will be installed Spring/Summer 2020. Be a part of a new beginning-start your Adventure here!
Tracey Stewart Roy J. Carver High Resolution Microscopy Facility Light and Electron Microscopy Room 0106 Molecular Biology Bldg. 2437 Pammel Dr. Iowa State University Ames, IA 50011-1079 515-294-3872
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Dear Lev, is this the High Voltage Unit, wich doesn't work or the supply of the HV-Unit (e.g. security circuit)? Overall Circuits of the Gatan Duomill I can send you a copy, Circuit Diagram of the HV-Unit I have never seen. Unfortunately Gatan seems to be strictly opposed to give circuit diagrams to customers even for old equipment. If the HV Unit is not working maybe I can help you with a unit in working order.
Best regards Winfried
Am So., 6. Okt. 2019 um 14:58Â Uhr schrieb {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } :
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My university is installing a new field emission SEM that will be used by a wide range of faculty in biology, chemistry, physics, geology, archeology, and ceramics. Undergraduate students will use the instrument under the supervision of their faculty advisors. An engineering physics professor and I (a geology professor) will be the primary managers of the facility. We are both reasonably competent, knowledgeable individuals, but we will have no dedicated technician and will never have budget for one.
I would like to minimize people accidentally screwing things up for the rest of us. I have protocols in mind to achieve this, but I know that people in this community are far, far more experienced than I am. Experience can be a good filter for identifying effective ideas vs. folly.
QUESTION: If you have experience managing an SEM facility used by diverse users, would you please share some of your successful (and unsuccessful) ideas that you’ve tried to minimize user harm to the instrument?
From codyhuey981ifbxi-at-gmail.com Tue Oct 8 07:16:07 2019 Return-Path: {codyhuey981ifbxi-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.162.202] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x98CG64o010167 for {microscopylistserverarchive7-at-microscopy.com} ; Tue, 8 Oct 2019 07:16:06 -0500 Message-ID: {515A02B5.08E5E489-at-gmail.com}
X-from: eyork-at-psemi.com
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Email: eyork-at-psemi.com Name: Evelyn York
Organization: pSemi Corporation
Title-Subject: [Filtered] Senior Technician, Failure Analysis Req Id 7671 - United States - California - San Diego - pSemi
Message: The Senior Technician, Failure Analysis, will primarily be tasked with running a variety of physical failure analysis activities to support the FA and Package engineering teams. As Senior Technician, the successful candidate will master the knowledge, skills, and competencies to work independently with other groups or team members to perform package construction analyses, preliminary inspections of devices submitted for FA, elemental composition analysis using SEM-EDS, and assist FA engineers as needed. A strong understanding of back-end packaging and module assembly processes, as well as stress, structural and mechanical analyses is heavily preferred. The company strongly prefers a driven team player with excellent communication skills as you will be working daily within a multifunctional group.
To learn more about this position go to pSemi.com
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Email: Hui_Yang1-at-uml.edu Name: Hui Yang
Organization: University of Massachusetts Lowell
Title-Subject: [Filtered] TEM Resources
Message: Hello all, I am applying for a research project at Fort Pierce, Florida. I'm going to need to do some TEM for my project. Does anybody know if there is a TEM facility/service in the area or within 3 hours drive?
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Title-Subject: [Filtered] folds in sections of cells grown on Transwell filters
Message: Hi, I am doing TEM of embryonic cells grown on polycarbonate membranes in Transwell dishes. The cells grow in layers of two or three cells. After fixation (aldehydes, osmium) I removed the filters with the cells on top, cut them into little pieces and proceed to dehydration and embedding in Eponate 12, placing the pieces on flat molds.
I am cutting (ultra thin, 60 nm) the in the plane perpendicular to the membrane. I get lots of folds, wrinkles in the that radiate from the membrane to the cells...a pity, the cells look really nice but it is hard to image because of the many folds...
Has anyone done TEM of cells in Transwell or other type of filters? Any suggestion to avoid the folds?
Thank you!!
AMALIA
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X-from: Smith, III, Julian P.S {smithj-at-winthrop.edu}
Hi, Kurt,
We're a primarily undergraduate institution with no recharge for in-house use.
Our microscope (W-gun) is fully automated and is turbo-pumped, so our primary goal is to protect the backscattered detector from damage or from someone popping the X-ray window by slamming the specimen drawer shut.
If I a PI wants his/her lab to use the SEM, I train the PI first. We have a basic SOP that requires use of a standard stub and holder, requires that the specimen not extend more than 3mm above the stub surface and not beyond the edge of the stub. Working distance is set at 20mm nominal (about 17mm in practice), and not decreased (increase is o.k., but the WD is set back to 20mm at the end of the session)
Once trained, the SOP is signed by the PI (whose department thereby assumes financial liability for damage), and the PI is allowed to use the microscope independently. The PI is allowed to train his/her students (again with the same SOP); before the students are allowed to use the scope independently, the PI has them sign off on the SOP, and he/she countersigns (again the PI's dept. is responsible for any damage)
I develop custom SOP's for PI's or students that need special conditions (specimen tilt, X-ray analysis at 10mm working distance, large specimens, etc), but the same conditions apply--the PI and his/her dept. agree to be responsible for damage
One advantage to this system is that the PI is thoroughly familiar with the instrument, and is responsible for training his/her own students.
We also don't have a technician, and if I had to train every student that uses the scope, I'd go insane.
Best, Julian
 On 10/8/19 2:43 AM, microscopy.listserver-at-gmail.com wrote:
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} } Thank you. } } Kurt Friehauf } } -------- } } Kurt Friehauf, Ph.D., P.G. } } Professor of Geology } } Department of Physical Sciences } } Kutztown University } } Kutztown, PA 19530 } } http://faculty.kutztown.edu/friehauf } } } ==============================Original Headers============================== } 18, 53 -- From microscopy.listserver-at-gmail.com Tue Oct 8 01:43:42 2019 } 18, 53 -- Received: from mail-wr1-f43.google.com (mail-wr1-f43.google.com [209.85.221.43]) } 18, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x986hgGd013914 } 18, 53 -- for {microscopy-at-microscopy.com} ; Tue, 8 Oct 2019 01:43:42 -0500 } 18, 53 -- Received: by mail-wr1-f43.google.com with SMTP id o18so17893124wrv.13 } 18, 53 -- for {microscopy-at-microscopy.com} ; Mon, 07 Oct 2019 23:50:19 -0700 (PDT) } 18, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 18, 53 -- d=gmail.com; s=20161025; } 18, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 18, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 18, 53 -- bh=/2Ms2ABwUlWhcVwvAi5TARdwcptP8Iql5LvzYLWF9JQ=; } 18, 53 -- b=oY7GB/Wc9HS6r1imk1VviseRDZqvdoPHvj4wLKP/B31D0r0PpVTGNpGCX3GuwusSEq } 18, 53 -- Z4K14vts1NhMynCv48TZWApZLCHO3JKq2WIThz+WkPBvBb8URaCs4bgNpP3ZmqXIEUup } 18, 53 -- aB5yUHfCyA6qu3d1UU/i/PWowIKmRza+LOhSpsb0F6CDDdOtpoyjG4YOM709nEFRuPFQ } 18, 53 -- WTzWIeOS9JChmlQLBvM6lOf6zkO3qBrZFxk9AIXCQcJ+JIM8dsvhrwJLyECmkX6eDZas } 18, 53 -- zYNIw1gGwBh4aMk1EBaA3iaTO3uMxxztoNvVZvGbtMPy6XwHdjYUpeZaAQHzfDka51D2 } 18, 53 -- Ctqw== } 18, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 18, 53 -- d=1e100.net; s=20161025; } 18, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 18, 53 -- :user-agent:mime-version:in-reply-to:content-language } 18, 53 -- :content-transfer-encoding; } 18, 53 -- bh=/2Ms2ABwUlWhcVwvAi5TARdwcptP8Iql5LvzYLWF9JQ=; } 18, 53 -- b=rgvPg1jzUjLbWqyEDAH2d8OLBBRuljRuX7ikQS7isRGt1+tlyPnH+saaa4TAN2WCPB } 18, 53 -- PpbY6UZw+5fzq2D1PVietfAl6zbqvxoU2RpM71GgtHjCyqKPIvekdV/EIZKVZHLytdR3 } 18, 53 -- 85HbNfwkrB+DZHnWcxQ0Uj81TROZ3OI/ZR1Zfy3WdY7fLkJFhU9pNNpom4ZWblZdbVLc } 18, 53 -- kvGdInKRomjoFq+bHjARc8XzY5GKy3U85DHRIfKB5WKLGZ/8jhupYVpi3jost5YKfz3M } 18, 53 -- kY8JAgCcjsMyqEosAW02GxSmI1GnqiDRPbdbyzZ13v4iC1lAx0mWfbB8fSCRdGoUbpm2 } 18, 53 -- bXcQ== } 18, 53 -- X-Gm-Message-State: APjAAAUXVG9pHsrB09z+svQfBOYjeCfF6NuUIN5U0dIKOuE5NN6dHbub } 18, 53 -- HAlOSspX17oNqyivC/FaLinbJ8617vw= } 18, 53 -- X-Google-Smtp-Source: APXvYqzXimWpCPIFMA+hg+/52g0aiTs4Y1GFH/dDdoRNWpWY4VTTsjs0OvgLWoiwfC2lWEIkci8UAw== } 18, 53 -- X-Received: by 2002:adf:f8cf:: with SMTP id f15mr24920653wrq.292.1570517418449; } 18, 53 -- Mon, 07 Oct 2019 23:50:18 -0700 (PDT) } 18, 53 -- Received: from Nestor-MacBookAir-Pro-2014-ElCapitan.local ([2001:6b0:4f:2801:296c:6e6e:9339:bcb9]) } 18, 53 -- by smtp.googlemail.com with ESMTPSA id q19sm46153241wra.89.2019.10.07.23.50.17 } 18, 53 -- for {microscopy-at-microscopy.com} } 18, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 18, 53 -- Mon, 07 Oct 2019 23:50:18 -0700 (PDT) } 18, 53 -- Subject: Fwd: Request advice for minimizing user error } 18, 53 -- References: {BN6PR01MB2562C6345E159F3E2257345DB0980-at-BN6PR01MB2562.prod.exchangelabs.com} } 18, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 18, 53 -- From: Microscopy Listserver {microscopy.listserver-at-gmail.com} } 18, 53 -- X-Forwarded-Message-Id: {BN6PR01MB2562C6345E159F3E2257345DB0980-at-BN6PR01MB2562.prod.exchangelabs.com} } 18, 53 -- Message-ID: {1f5120f1-9ea8-6e31-f821-fded9938a3d5-at-gmail.com} } 18, 53 -- Date: Tue, 8 Oct 2019 08:50:13 +0200 } 18, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.14; rv:60.0) } 18, 53 -- Gecko/20100101 Thunderbird/60.9.0 } 18, 53 -- MIME-Version: 1.0 } 18, 53 -- In-Reply-To: {BN6PR01MB2562C6345E159F3E2257345DB0980-at-BN6PR01MB2562.prod.exchangelabs.com} } 18, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 18, 53 -- Content-Language: en-US } 18, 53 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers==============================
-- Julian P.S. Smith III Director, Winthrop Microscopy Facility Dept. of Biology Winthrop University 349 Columbia Ave Rock Hill, SCĂŠ 29733
803-323-2111 x6427 (vox) 803-323-3448 (fax) 803-524-2347 (cell) Research Website www.birdnest.org/smithj Personal Website www.rociada-east.net
I am in a similar position to you, with the exception that there is a technician hired to run the EMs and facility as a whole (me). For training, I do strongly encourage, and where I can, require that any student that wants to use one of our EMs or other microscopes take the relevant class. If you do not have classes in microscopy -- SEM in your instance -- I strongly encourage you to start one. This is perhaps the best way to make sure users are properly trained. For those users who can't take a class, then I do train them one-on-one, with particular emphasis on those parts of instrument operation where they could cause damage. For these steps, typically sample loading and removal, I'll make the user practice doing the step, and make it very clear that they are responsible for any damage and will be billed for it. Being told the price of a new specimen rod for the TEM or BSE detector for the SEM is a strong inducement to do things right.
Use of the microscopes is also restricted to hours when I am present until I am comfortable with their abilities. With demonstrated competence, users can work after hours.
Since instruments these days are generally well protected by safety switches and software, the possible failure points are much fewer. Unlike the Old Days us GOMs happily remember, with the manual vacuum valves and so on ... This makes it easier to train users. Then, one has to have some belief in the users. Somewhat less comfortable than believing in the reliability of safety switches and software protections, but possible with good training, carefully going over what a user can break or damage, and the expensive consequences they get to pay if they do damage something.
I also have service contracts on the EMs and confocals. This is another major help.
TL/DR: Classes, individual training, and being clear about responsibility for damages, including paying for damages.
Phil ------------- Philip Oshel Imaging Facility Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 989 774-3576 office
Dear Kurt, We run a core facility here TEM's FESEM Confocals etc. and we have a consistent training pogram (by qualified staff) with certifications and a probationary period (10 hrs during normal work hours so staff is available). As far as the FESEM troubles, we once had a disaster with loose sample material destroying a turbo molecular pump. Since then we insist on making sure nano materials are not in such excess that they can fall into the pump blades during pumping and/or venting. To achieve this we usually blow loose particles off stubs with dry nitrogen or an air canister. We do not allow derivative training, and our staff are mindful of violators (which would have to be re-trained and placed back on probation). Sincerely, Michael Delannoy Johns Hopkins SOM Microscope Facility
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Tuesday, October 08, 2019 2:51 AM To: Michael Delannoy
X-from: Friehauf, Kurt {friehauf-at-kutztown.edu}
Dear Microscopists,
My university is installing a new field emission SEM that will be used by a wide range of faculty in biology, chemistry, physics, geology, archeology, and ceramics. Undergraduate students will use the instrument under the supervision of their faculty advisors. An engineering physics professor and I (a geology professor) will be the primary managers of the facility. We are both reasonably competent, knowledgeable individuals, but we will have no dedicated technician and will never have budget for one.
I would like to minimize people accidentally screwing things up for the rest of us. I have protocols in mind to achieve this, but I know that people in this community are far, far more experienced than I am. Experience can be a good filter for identifying effective ideas vs. folly.
QUESTION: If you have experience managing an SEM facility used by diverse users, would you please share some of your successful (and unsuccessful) ideas that you've tried to minimize user harm to the instrument?
Hi AMALIA ... The cause of the folds may be the thinness of your sections ... try cutting at 80nm .... if you need your sections to be so thin you can try grids with formvar or carbon ... I hope you correct your problem .. Regards...
} El 08/10/2019, a las 11:52, microscopy.listserver-at-gmail.com } {mailto:microscopy.listserver-at-gmail.com} escribió: } } Hi, I am doing TEM of embryonic cells grown on polycarbonate } membranes in Transwell dishes. The cells grow in layers of two or three } cells. After fixation (aldehydes, osmium) I removed the filters with the } cells on top, cut them into little pieces and proceed to dehydration and } embedding in Eponate 12, placing the pieces on flat molds. } } I am cutting (ultra thin, 60 nm) the  in the plane perpendicular to the } membrane. I get lots of folds, wrinkles in the that radiate from the } membrane to the cells...a pity, the cells look really nice but it is } hard to image because of the many folds... } } Has anyone done TEM of cells in Transwell or other type of filters? Any } suggestion to avoid the folds? } } Thank you!! } } AMALIA
We do not allow derivative training. It is like the child's game of telephone. What one student thinks is important and passes on to another student is often not the same set of information that I would pass along. I warn trained users that I see doing it. Their trainees are not allowed to operate the SEM by themselves until they have gone through official training.
The new Schottky-gun field emission SEMs are quite user friendly, maybe friendlier than W-gun SEMs. Of course, I think some brands or models are friendlier than others. They generally now have lots of interlocks to prevent damage although it is still possible to drive samples into detectors. For instance, there is an issue with software on one model that must have been written in Europe. When you want to set the stage height to 15 mm, be sure to enter "15" or "15.0". Do not enter "15." because it will be taken as 15.000 (i.e., 15 thousand according to European rules). That will definitely cause a problem.
I would definitely get a chamber camera so users can see where they are driving the stages. I cannot bear the thought of students working blind.
I also highly recommend a navigation camera or at least the ability to register with an external image. It makes it much easier finding the area of interest in the case of large samples or multiple samples.
As one who has been using SEM and EDS for long time and now manages a facility, I recommend you have an experienced applications person on hand. I don't think that you want that to be you. You probably don't want to be sucked into the mundane, repetitive questions. You need to have someone who knows their microscopy in general and the peculiarities of the microscope in particular. For example, I find lots of users who rely on secondary electron imaging at 20 kV because that is all they know. I try to push them towards backscattered electron imaging and using lower voltages and to think about what would be best for their samples.
I see lots of errors on teaching scopes where there is not expert support. Students usually work on their own and it is rare to get a professor into the lab. They may not have taken the course but I do not suppose most courses give them enough practical training for students to determine what would be best for their samples. I respect professors for the knowledge of their field, but I have yet to meet one who was a top microscopist. I find myself needing to offer counsel on the scientific side of things. I don't assume that what a student found in a paper was the best way to characterize the material.
Grad students are often tagged to be the resident expert. That may be better than nothing; however, they often have a rather short tenure and are interested in working on their own research. There is usually not much of a hand-off to the next grad student.
Be careful to develop a solid SOP. I think a short but complete checklist is better than an exhaustive SOP with pictures that covers everything. If it is too long, users won't refer back to it. I want users to help themselves. The necessary work flow is on the 2-page check-list. I make a point of sending them back to the point they missed when they encounter problems. It is usually because they skipped a step or took steps out of order. Soon enough, they learn to do it by themselves.
Warren Straszheim Materials Analysis and Research Lab Iowa State University
-----Original Message----- X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Tuesday, October 08, 2019 1:44 AM To: Straszheim, Warren E [BIOTC] {wesaia-at-iastate.edu}
X-from: Friehauf, Kurt {friehauf-at-kutztown.edu}
Dear Microscopists,
My university is installing a new field emission SEM that will be used by a wide range of faculty in biology, chemistry, physics, geology, archeology, and ceramics. Undergraduate students will use the instrument under the supervision of their faculty advisors. An engineering physics professor and I (a geology professor) will be the primary managers of the facility. We are both reasonably competent, knowledgeable individuals, but we will have no dedicated technician and will never have budget for one.
I would like to minimize people accidentally screwing things up for the rest of us. I have protocols in mind to achieve this, but I know that people in this community are far, far more experienced than I am. Experience can be a good filter for identifying effective ideas vs. folly.
QUESTION: If you have experience managing an SEM facility used by diverse users, would you please share some of your successful (and unsuccessful) ideas that you've tried to minimize user harm to the instrument?
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both wfschneider-at-wisc.edu, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Email: wfschneider-at-wisc.edu Name: Bil Schneider
Organization: UW Madison Geosciences
Title-Subject: [Filtered] Outreach session at M&M 2020
Message: I'd posted a query last August about interest in a session at M&M 2020 for day to day laboratory operations. I received many responses that showed plenty of interest in a session and several suggestions for some of the topic/discussion points. In fact the recent post by Kurt Friehauf from Kutztown University seeking advice from the larger community about minimizing user error and training on a new SEM is one of the upcoming sessions talking points exactly. The session will be in the "Outreach Sessions" when the M&M 2020 itinerary is published: X34 Management and Operation of Microscopy and Microanalysis Facilities (Organized by the Facilities Operations Management Focused Interest Group – FOM FIG) ORGANIZERS: Luisa Amelia Dempere, University of Florida John Fournelle, University of Wisconsin-Madison William Schneider, University of Wisconsin-Madison • Keeping the tools running day to day • Supporting users: training and optimizing tool time • Developing the optimal rate structure to get science done • Tracking tool usage for validating fund streams • Multi-user models for tool usage and access • Service contracts (OEM and 3rd party) vs DIY
Any other suggestions or modification of our talking points is appreciated. Hope to see you in Milwaukee!
Cheers, Bil Schneider SEM Lab Manager UW Madison Geosciences
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Hi Amalia, Recently I've experienced the same problem with chemically fixed cells on transwell (PES) filters and on track-etched polycarbonate membranes, dehydrated in ethanol and embedded in EPON (Mollenhauer based mixture, medium-hard). In my case sectioning thicker doesn't help that much,the wrinkles are already visible when the sections come off the diamond knife. only in sections below 40nm there is less wrinkling. Additional stretching with chloroform vapor doesn't help. I haven't tried stretching with a heat pen or adding a drop of 100% ethanol to the waterbath (I avoid the latter since it makes it almost impossible to section ribbons). The whole behavior of the material during sectioning appears to be due to a difference in sectioning properties between the resin and the membrane (even though it is also infiltrated with resin). While the resin compresses somewhat during sectioning but stretches again on the waterbath, the filter doesn't follow this stretching and thus wrinkles are formed. The interesting thing is that these trans-well filters have been successfully used for HPF- freeze-substitution and resin embedding (Morphew et al. Journal of Microscopy, Vol. 212, Pt 1 October 2003) without this wrinkling being a problem. It can be that for these filters dehydration in acetone will allow for a better resin infiltration of the filter or that a harder resin mixture (less compression during sectioning)may prevent the wrinkles from forming.
best of luck! Rob
-- Dr. Rob Mesman Post-Doc Department of Microbiology Faculty of Science Radboud University Nijmegen Heyendaalseweg 135 NL-6525 AJ Nijmegen The Netherlands
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Title-Subject: [Filtered] folds in sections of cells grown on Transwell filters
Message: Hi, I am doing TEM of embryonic cells grown on polycarbonate membranes in Transwell dishes. The cells grow in layers of two or three cells. After fixation (aldehydes, osmium) I removed the filters with the cells on top, cut them into little pieces and proceed to dehydration and embedding in Eponate 12, placing the pieces on flat molds.
I am cutting (ultra thin, 60 nm) the in the plane perpendicular to the membrane. I get lots of folds, wrinkles in the that radiate from the membrane to the cells...a pity, the cells look really nice but it is hard to image because of the many folds...
Has anyone done TEM of cells in Transwell or other type of filters? Any suggestion to avoid the folds?
Thank you!!
AMALIA
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==============================Original Headers============================== 15, 23 -- From R.Mesman-at-science.ru.nl Thu Oct 10 02:33:06 2019 15, 23 -- Received: from smtp3.science.ru.nl (smtp3.science.ru.nl [131.174.30.193]) 15, 23 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x9A7X5oh014505 15, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 10 Oct 2019 02:33:05 -0500 15, 23 -- Received: from roundcube.science.ru.nl (weber.science.ru.nl [131.174.30.199]) 15, 23 -- by smtp3.science.ru.nl (8.15.2/5.32) with ESMTP id x9A7dkiX025097; 15, 23 -- Thu, 10 Oct 2019 09:39:46 +0200 15, 23 -- Received: from n018157.science.ru.nl ([131.174.18.157]) 15, 23 -- by roundcube.science.ru.nl 15, 23 -- with HTTP (HTTP/1.1 POST); Thu, 10 Oct 2019 09:39:46 +0200 15, 23 -- MIME-Version: 1.0 15, 23 -- Content-Type: text/plain; charset=US-ASCII; 15, 23 -- format=flowed 15, 23 -- Content-Transfer-Encoding: 7bit 15, 23 -- Date: Thu, 10 Oct 2019 09:39:46 +0200 15, 23 -- From: Rob Mesman {R.Mesman-at-science.ru.nl} 15, 23 -- To: amaliapasolli-at-gmail.com, Microscopy-at-microscopy.com 15, 23 -- Subject: Re: folds in sections of cells grown on Transwell filters 15, 23 -- Message-ID: {1d613aa9228f0633b73e98f7c9fe9cc6-at-science.ru.nl} 15, 23 -- X-Sender: R.Mesman-at-science.ru.nl 15, 23 -- User-Agent: Roundcube Webmail/1.3.6 15, 23 -- X-Spam-Score: 1 (*) ALL_TRUSTED,BAYES_50 15, 23 -- X-Scanned-By: mimedefang version 2.83 on 131.174.30.193 131.174.30.60 (smtp3) ==============================End of - Headers==============================
Dear list, dear Amalia, (apologies for lengthiness)
Since I didn’t receive the original request of Amalia Pasolli via MSA-Listserver this time, I use the reply by Prof. Dr. Mesman to add my 2 cents to the problem:
„The whole behavior of the material during sectioning appears to be due to a difference in sectioning properties between the resin and the membrane (even though it is also infiltrated with resin).“ Seconding that (Dr. Mesman's) idea: That's the most probable cause of your problem.
Dr. Mesman points you [regarding the developments in EM over the last 10-16 years!] to an "ancient" paper and protocol ["Morphew et al. Journal of Microscopy, Vol. 212, Pt 1 October 2003 *) without this wrinkling being a problem."]
Quote from their Material&Methods section: "} Embedding and sectioning { Filter discs with associated cells were infiltrated with epoxy resin (EPOX-Araldite) (EMS) and flat embedded following a method previously described (Reymond & Pickett-Heaps, 1983). Briefly, filter discs were transferred to glass slides coated with PTFE Release Agent (Miller-Stevenson, Danbury, CT, U.S.A.) that supported a flat pool of ~20 mL of embedding resin. Another coated slide was then placed over the first and gently pressed down, so the resin layer would be as thin as possible. The resin was polymerized at 60 °C for 48 h. Following polymerization, the slides were separated with a razor blade, exposing the resin wafer that contained the embedded filters still attached to one of the slides (Fig. 1d). The filter discs (Fig. 1d,arrow) were examined by phase contrast LM, and cells of interest were scored with a diamond scribe. A no. 11 scalpel was used to excise a piece of the filter (a square ~3 mm on a side) (Fig. 1d,arrowhead) that contained the scored cell, which was sub-sequently remounted with epoxy glue onto a blank microtome stub in an orientation suitable for microtomy. Mitotic PtK cells were mounted for en face sectioning, and MDCK cells were orientated perpendicular to the growth substrate" (end of quote)
*) Original Article: "The use of filter membranes for highâ€pressure freezing of cell monolayers" https://onlinelibrary.wiley.com/doi/abs/10.1046/j.1365-2818.2003.01231.x Their section thicknesses (as of the descriptions in their article) were ~ 150 nm for "semithick" sections, or ~ 70nm for 'ultrathin sections', respectively, achieved with a diamond knife of a renowned diamond knife producer.
The Fig. 2a in their publication shows- as by the legend: (quote): "Fig. 2. Transmission electron micrographs of cells high-pressure frozen from filter discs. (a) Mitotic PtK ell in a field of interphase cells shows the interface between the cells and the substrate. Pores of the membrane filter (arrow) are visible. ....",
but looking a bit closer in that Fig.2a one (as a bench worker and "imagineur"=observer/contemplator on the screen or printed photo) can see that they must have had 'some' problem with wrinkles and folding too...(I guess: a really fortunate - overview- illustration of the cell located at the filter membrane.... across the membrane contained within ~4µm length I count 3 visible folds within the image).
Have you seen "semithin"(=~0.5 - 1µm thickness) or "semithick" (= ~=ca. 150nm thickness) sections with regard to their 'wrinkling tendency' and real wrinkling ON your glass slide (including staining for LM) and the (/a possible improving?) effect of variation of the knife /cutting angle, cutting speed, water-level at the knife-cutting edge?
It might be interesting - before discussing eventually about the necessary hardness [or, as in other cases, even right softness] of the embedding resin to know the recipe/protocol for making / of your EPONATE 12 resin [guessing, it might be ] to evaluate then the possible differences to the EPOX-ARALDITE [EMS]-resin used in the MORPHEW/McINTOSH-2003 article (if you can't access easily or don't have a subscription to J Microscopy, it / I might be possible to send you a copy of that article very rapidly).
The fore-mentioned authors refer to another ('older') technique published by REYMOND & Pickett-Heaps, 1983 [: 'Routine flat-embedding method for electron microscopy of microorganisms allowing selection and precicely oriented sectioning of single cells by light microscopy'. J. Microsc. 130, 79 – 89], cf.: https://onlinelibrary.wiley.com/doi/10.1111/j.1365-2818.1983.tb04200.x , which - for sure - is quite fascinating regarding their technique but unfortunately they dwell on the subject "resin" or "resin-hardness" at the following statement: (quote from their article page -81- ): " 1. The biological material, already fixed, dehydrated and embedded in liquid resin (Spurr’s or Epon, for example) is placed on the surface of one microscope slide previously covered with the release agent. " So- no recipe or protocol for a preferred resin or resin mixture can be 'derived' from that article source.
It can be discussed further about the quality composition, achieved hardness of the used resins as mentioned: EPOX-Araldite (MORPHEW & McINTOSH,2003) and EPONATE 12 (TM of T.P-Pel....., = generic replacement for Epon 812 formerly a Sh....Chem. Co product), as in the respective data sheet / Technical Note (quote) "ONE MIX FORMULATIONS" there are presented 3 'possible' variants: SOFT - MEDIUM - HARD (variables also: 2 types of accelerators, and - additionally: variable details of infiltration and polymerisation): .... "The ratio in moles of MNA: DDSA determines the block hardness......" "... SOFT means: resin mixture contains no MNA, HARD means: resin mixture contains NO DDSA ..."
As a possible 'last' point besides description of the "sampling steps" ["...removed the filters with the cells on top, cut them into little pieces and proceed to dehydration... ] to be thrown in this discussion: Also it might be of interest to know which 'knife' you use for ultrathin sectioning: glass or diamond?
Best wishes and as ever:
{ {Stay safe and have a successful day/rest of the week} }
Wolfgang MUĂź(MUSS) Retired Member of MSA SALZBURG-AUSTRIA
DISCLAIMER: Eventually mentioned Company names incidentally...no affiliation to AND no financial interest. Abbreviated Company names used to fulfill "Terms of list server use" -The names of those companies can be diclosed if necessary. Copies of the mentioned papers are saved in my works collection and perhaps - ONLY for personal USE - can be forwarded on personal request (included a very ancillary 'working pamphlet' by the famous but unfortunately late [+ 12/6/2018) Prof. Dr. Helmuth Sitte: "Ultramicrotomy-Common problems and Mistakes, Type-written Manuscript 1981" and e.g. 'Guide to Sectioning on the R-J-Ultracut E -Ultramicrotome' by Shannon Modla - BioImaging Center - Delaware Biotechnology Institute (pdf, created presumably 2006-2007) with multiple and valuable information on trouble checking....
========================================================================================== Von: R.Mesman-at-science.ru.nl [mailto:R.Mesman-at-science.ru.nl] Gesendet: Donnerstag, 10. Oktober 2019 09:34 An: wij.muss-at-aon.at Betreff: [Microscopy] Re: folds in sections of cells grown on Transwell filters [polycarbonate membranes in Transwell dishes. ...] + the Original REQUEST by AMALIA PASOLLI ---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Hi Amalia, Recently I've experienced the same problem with chemically fixed cells on transwell (PES) filters and on track-etched polycarbonate membranes, dehydrated in ethanol and embedded in EPON (Mollenhauer based mixture, medium-hard).
In my case sectioning thicker doesn't help that much, the wrinkles are already visible when the sections come off the diamond knife. Only in sections below 40nm there is less wrinkling. Additional stretching with chloroform vapor doesn't help. I haven't tried stretching with a heat pen or adding a drop of 100% ethanol to the waterbath (I avoid the latter since it makes it almost impossible to section ribbons).
The whole behavior of the material during sectioning appears to be due to a difference in sectioning properties between the resin and the membrane (even though it is also infiltrated with resin).
While the resin compresses somewhat during sectioning but stretches again on the waterbath, the filter doesn't follow this stretching and thus wrinkles are formed. The interesting thing is that these trans-well filters have been successfully used for HPF- freeze-substitution and resin embedding (Morphew et al. Journal of Microscopy, Vol. 212, Pt 1 October 2003) without this wrinkling being a problem.
It can be that for these filters dehydration in acetone will allow for a better resin infiltration of the filter or that a harder resin mixture (less compression during sectioning) may prevent the wrinkles from forming.
best of luck! Rob
-- Dr. Rob Mesman Post-Doc Department of Microbiology Faculty of Science Radboud University Nijmegen Heyendaalseweg 135 NL-6525 AJ Nijmegen The Netherlands --------------------------------------------------------------------------- original message: X-from: amaliapasolli-at-gmail.com This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both -gmail.com, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom --------------------------------------------------------------------------- Email: amaliapasolli-at-gmail.com Name: Amalia Pasolli Organization: The Rockefeller University Title-Subject: folds in sections of cells grown on Transwell filters Message: Hi, I am doing TEM of embryonic cells grown on polycarbonate membranes in Transwell dishes. The cells grow in layers of two or three cells.
After fixation (aldehydes, osmium) I removed the filters with the cells on top, cut them into little pieces and proceed to dehydration and embedding in Eponate 12, placing the pieces on flat molds.
I am cutting (ultra thin, 60 nm) the in the plane perpendicular to the membrane. I get lots of folds, wrinkles in the that radiate from the membrane to the cells... a pity, the cells look really nice but it is hard to image because of the many folds...
Has anyone done TEM of cells in Transwell or other type of filters? Any suggestion to avoid the folds?
Thank you!!
AMALIA
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} } Thank you. } } Kurt Friehauf } } -------- } } Kurt Friehauf, Ph.D., P.G. } } Professor of Geology } } Department of Physical Sciences } } Kutztown University } } Kutztown, PA 19530 } } http://faculty.kutztown.edu/friehauf } } } ==============================Original Headers============================== } 18, 53 -- From microscopy.listserver-at-gmail.com Tue Oct 8 01:43:42 2019 } 18, 53 -- Received: from mail-wr1-f43.google.com (mail-wr1-f43.google.com [209.85.221.43]) } 18, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x986hgGd013914 } 18, 53 -- for {microscopy-at-microscopy.com} ; Tue, 8 Oct 2019 01:43:42 -0500 } 18, 53 -- Received: by mail-wr1-f43.google.com with SMTP id o18so17893124wrv.13 } 18, 53 -- for {microscopy-at-microscopy.com} ; Mon, 07 Oct 2019 23:50:19 -0700 (PDT) } 18, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 18, 53 -- d=gmail.com; s=20161025; } 18, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 18, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 18, 53 -- bh=/2Ms2ABwUlWhcVwvAi5TARdwcptP8Iql5LvzYLWF9JQ=; } 18, 53 -- b=oY7GB/Wc9HS6r1imk1VviseRDZqvdoPHvj4wLKP/B31D0r0PpVTGNpGCX3GuwusSEq } 18, 53 -- Z4K14vts1NhMynCv48TZWApZLCHO3JKq2WIThz+WkPBvBb8URaCs4bgNpP3ZmqXIEUup } 18, 53 -- aB5yUHfCyA6qu3d1UU/i/PWowIKmRza+LOhSpsb0F6CDDdOtpoyjG4YOM709nEFRuPFQ } 18, 53 -- WTzWIeOS9JChmlQLBvM6lOf6zkO3qBrZFxk9AIXCQcJ+JIM8dsvhrwJLyECmkX6eDZas } 18, 53 -- zYNIw1gGwBh4aMk1EBaA3iaTO3uMxxztoNvVZvGbtMPy6XwHdjYUpeZaAQHzfDka51D2 } 18, 53 -- Ctqw== } 18, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 18, 53 -- d=1e100.net; s=20161025; } 18, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 18, 53 -- :user-agent:mime-version:in-reply-to:content-language } 18, 53 -- :content-transfer-encoding; } 18, 53 -- bh=/2Ms2ABwUlWhcVwvAi5TARdwcptP8Iql5LvzYLWF9JQ=; } 18, 53 -- b=rgvPg1jzUjLbWqyEDAH2d8OLBBRuljRuX7ikQS7isRGt1+tlyPnH+saaa4TAN2WCPB } 18, 53 -- PpbY6UZw+5fzq2D1PVietfAl6zbqvxoU2RpM71GgtHjCyqKPIvekdV/EIZKVZHLytdR3 } 18, 53 -- 85HbNfwkrB+DZHnWcxQ0Uj81TROZ3OI/ZR1Zfy3WdY7fLkJFhU9pNNpom4ZWblZdbVLc } 18, 53 -- kvGdInKRomjoFq+bHjARc8XzY5GKy3U85DHRIfKB5WKLGZ/8jhupYVpi3jost5YKfz3M } 18, 53 -- kY8JAgCcjsMyqEosAW02GxSmI1GnqiDRPbdbyzZ13v4iC1lAx0mWfbB8fSCRdGoUbpm2 } 18, 53 -- bXcQ== } 18, 53 -- X-Gm-Message-State: APjAAAUXVG9pHsrB09z+svQfBOYjeCfF6NuUIN5U0dIKOuE5NN6dHbub } 18, 53 -- HAlOSspX17oNqyivC/FaLinbJ8617vw= } 18, 53 -- X-Google-Smtp-Source: APXvYqzXimWpCPIFMA+hg+/52g0aiTs4Y1GFH/dDdoRNWpWY4VTTsjs0OvgLWoiwfC2lWEIkci8UAw== } 18, 53 -- X-Received: by 2002:adf:f8cf:: with SMTP id f15mr24920653wrq.292.1570517418449; } 18, 53 -- Mon, 07 Oct 2019 23:50:18 -0700 (PDT) } 18, 53 -- Received: from Nestor-MacBookAir-Pro-2014-ElCapitan.local ([2001:6b0:4f:2801:296c:6e6e:9339:bcb9]) } 18, 53 -- by smtp.googlemail.com with ESMTPSA id q19sm46153241wra.89.2019.10.07.23.50.17 } 18, 53 -- for {microscopy-at-microscopy.com} } 18, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 18, 53 -- Mon, 07 Oct 2019 23:50:18 -0700 (PDT) } 18, 53 -- Subject: Fwd: Request advice for minimizing user error } 18, 53 -- References: {BN6PR01MB2562C6345E159F3E2257345DB0980-at-BN6PR01MB2562.prod.exchangelabs.com} } 18, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 18, 53 -- From: Microscopy Listserver {microscopy.listserver-at-gmail.com} } 18, 53 -- X-Forwarded-Message-Id: {BN6PR01MB2562C6345E159F3E2257345DB0980-at-BN6PR01MB2562.prod.exchangelabs.com} } 18, 53 -- Message-ID: {1f5120f1-9ea8-6e31-f821-fded9938a3d5-at-gmail.com} } 18, 53 -- Date: Tue, 8 Oct 2019 08:50:13 +0200 } 18, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.14; rv:60.0) } 18, 53 -- Gecko/20100101 Thunderbird/60.9.0 } 18, 53 -- MIME-Version: 1.0 } 18, 53 -- In-Reply-To: {BN6PR01MB2562C6345E159F3E2257345DB0980-at-BN6PR01MB2562.prod.exchangelabs.com} } 18, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 18, 53 -- Content-Language: en-US } 18, 53 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers============================== }
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Email: michelle.plue-at-duke.edu Name: Michelle
Organization: Duke University
Title-Subject: [Filtered] Cryo TEM Position Available
Message: Hello All. Duke University has a position open for a Cryo-TEM scientist - https://careers.duke.edu/job/Durham-RESEARCH-&-DEV-ENGINEER-III%2C-Cryo-EM-Facility-Team-Leader-NC-27710/594681200/
Please contact me at michelle.plue-at-duke.edu for more information about the position.
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From michhyma42hiyz-at-gmail.com Fri Oct 11 23:28:35 2019 Return-Path: {michhyma42hiyz-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.162.227] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x9C4SYt9000694 for {microscopylistserverarchive7-at-microscopy.com} ; Fri, 11 Oct 2019 23:28:35 -0500 Message-ID: {43D1A3E7.0CBF6C20-at-gmail.com}
X-from: Lee Cohen-Gould {lcgould-at-med.cornell.edu}
Hi All-
In response to Amalia Pisolli's questions about working with cell on Transwell filters, I published a short paper in Microscopy Today a few years ago about embedding cell monolayers, both in flasks and on Transwells. doi:10.1017/S1551929513000485 MT may 2013. There is a typo in the resin formula: NMA should be 6.0 g, but otherwise, this has worked well for me
The resin is different in the filters than in the cells, but this method has worked well for me for many years. It's the only reason I still keep any propylene oxide in the lab. Acetonitrile, which we've switched to for most purposes, doesn't make the filters curl.
Does anyone happen to have a manual for a Lipshaw Model 40A microtome hanging around? I would like (need) a photocopy or pdf/doc file (or original) of the manual. I'm keeping one going for our light microscopy class, but it's beginning to get cranky. Much like myself ...
Phil ------------- Philip Oshel Imaging Facility Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 989 774-3576 office
Status: This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both jeparker-at-som.umaryland.edu, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Email: jeparker-at-som.umaryland.edu Name: Jeff Parker
Organization: Univeristy of Maryland
Title-Subject: [Filtered] En Bloc Stain
Message: Our EM Lab currently utilizes Sodium Barbital in our En Bloc Stain. Does anyone know of a substitute for Sodium Barbital and would you be willing to share your protocol. Thank you
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Message: Can someone direct me to a good source of literature for sample prep/stain and or contrasting methods for eyes, lizards, other non-murine models for imaging in a MicroCT.
Thanks! John S Univ. of Georgia
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as Sodium Barbital (Nembutal) usually is used as an anesthetic prior to killing e.g. euthanizing experimental animals like mice or rats (and I've never read or heard about adding such anesthetic in an en bloc stain used for already fixed trimmed specimen tissue blocks IMHO you might reveal a bit more detailed your methodological approach.
If you use(d) -sodium barbital - HCl- (or ,Veronal'-[Barbital]) BUFFER (made with sodium diethylbarbiturate) and want to replace that substance you might disclose the previously used en bloc stain protocol to be able to help you with any meaningful solution of your problem. If you just want to change the buffer system we need to know which en bloc stain component you prefer to use (e.g. for UO2Ac en bloc after fixation with aldehydes in PO4-buffers the ,maleate buffer sequence' [washing first with maleate buffer and in the last step in UO2Ac+Maleate buffer) would be a realistic option. If other en bloc stains you have in mind, please specify). Veronal-Barbital-buffer solutions: cf: (https://www.fishersci.com/us/en/catalog/search/products?keyword=barbital)
Best regards,
Wolfgang MUSS Retired member of MSA SALZBURG, AUSTRIA
_________________________________________________________________________ Von: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Gesendet: Montag, 14. Oktober 2019 22:46 An: wij.muss-at-aon.at Betreff: [Microscopy] En Bloc Stain ---------------------------------------------------------------------------- - The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
please copy to both jeparker-at-som.umaryland.edu, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom --------------------------------------------------------------------------- Email: jeparker-at-som.umaryland.edu Name: Jeff Parker Organization: Univeristy -University of Maryland Title-Subject: En Bloc Stain Message:
Our EM Lab currently utilizes Sodium Barbital in our En Bloc Stain. Does anyone know of a substitute for Sodium Barbital and would you be willing to share your protocol. Thank you
I was recently given an antique Minot Rotary Microtome. Does anyone have any instructions on how to use it? Many thanks Elaine
Dr. Elaine C. Humphrey Fellow of Microscopy Society of America Past President Microscopical Society of Canada Advanced Microscopy Facility Bob Wright Science Centre A015 University of Victoria, Canada Lab: 250-853-3968 cell: 250-886-2068 https://www.uvic.ca/research/advancedmicroscopy https://www.uvicwomeninscience.com/interviews/2018/12/21/dr-elaine-humphrey
From tammyhoward072zlmac-at-gmail.com Tue Oct 15 10:45:28 2019 Return-Path: {tammyhoward072zlmac-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.162.224] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x9FFjQqV008282 for {microscopylistserverarchive7-at-microscopy.com} ; Tue, 15 Oct 2019 10:45:27 -0500 Message-ID: {6C4BAA4E.DC30FB56-at-gmail.com}
X-from: Rob Rankin {robrankin-at-rankinbiomed.com}
I strongly advise not to throw good money and time at it. It is too old to support with parts or service.
Rob
On Tue, Oct 15, 2019, 2:20 AM {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote:
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I was recently given an antique Minot Rotary Microtome. Does anyone have any instructions on how to use it? Many thanks Elaine
Dr. Elaine C. Humphrey Fellow of Microscopy Society of America Past President Microscopical Society of Canada  Advanced Microscopy Facility Bob Wright Science Centre A015 University of Victoria, Canada  Lab: 250-853-3968 cell: 250-886-2068 https://www.uvic.ca/research/advancedmicroscopy
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While not exactly what you are after I have found the below reference a good start. Haven't specifically done eyes myself. Water based iodine is strongly represented in the research, but our own testing showed it to be somewhat damaging in the larger long term staining protocols and we got better contrasting with alcoholic versions. If you don't get any responses Morgan Chase at American Museum in NY has just set up a slack channel for µCT to replicate the functionality of the microscopy listserver for x-ray CT and I can send you a link to join or just post on your behalf.
BMC Physiology Methodology article Open Access MicroCT for comparative morphology: simple staining methods allow high-contrast 3D imaging of diverse non-mineralized animal tissues Brian D Metscher Address: Department of Theoretical Biology, Gerd Müller, head, University of Vienna, Althanstraße 14, 1090 Austria
Scott Whittaker Lab Manager Scientific Imaging P.O. Box 37012 MRC-104 Room W150 Washington, DC 20013-7012 w 202.633.0891 whittaks-at-si.edu
SMITHSONIAN INSTITUTION NATIONAL MUSEUM OF NATURAL HISTORY Facebook | Twitter | Instagram
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X-from: Elaine Humphrey {ech-at-uvic.ca}
Hi Rob I agree on making it a part of the infrastructure. The modern microtomes make life much easier.
But it is an antique so on a purely interested level, I wondered how it worked. Since the only part that is missing is the chuck holder, it is not going to be a useable machine unless someone has one to complete it. I think I've figured it out now but it would have been nice to see the original instructions.
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Dear All,
I wonder what features are absolutely necessary for SEM machine that will be used to do Array Tomography? Array tomography - ultrahin sections ribbons are collected on silicon wafers and these wafers are analyzed with SEM using backscattered (I assume) electrons. Then images of sections can be reconstructed into 3D volume.
Best regards,
Alex
-- Dr. Aleksandr Mironov MD, PhD Senior Experimental Officer D.1527, M.Smith Building EM Core Facility School of Biological Sciences, Faculty of Biology Medicine and Health University of Manchester Oxford Road Manchester M13 9PT UK
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Email: jhyun-at-gatan.com Name: John Hyun
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Title-Subject: [Filtered] The Second International Symposium on Advanced Microscopy and Spectroscopy (ISAMS-2) & The UCI School for Transmission Electron Microscopy (UCI-STEM)
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University of California, Irvine ISAMS-2: December 8-10, 2019 UCI-STEM: December 11-13, 2019 https://sites.uci.edu/isams2/
We are delighted to host our second annual International Symposium on Advanced Microscopy and Spectroscopy (ISAMS-2). The symposium will focus on the retrieval of hidden structural, chemical, electronic, orbital and spin information at the sub-atomic scale by leveraging the novel instruments that can capture electron scattering, and energy loss processes at unprecedented time and energy scales. Topical subjects of in situ microscopy such as gas and liquid cells, electrical and mechanical manipulation, and radiation damage will also be discussed.
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Here are a couple of publications that should provide you some useful information on soft tissue staining for micro-CT studies on non-murine vertebrates. P. Gugnac and N. Kley, J. Exp. Zool. (Mol. Dev. Evol.) 322B:166-176, 2014.
B. Metscher, Microscopy and Analysis, pp 13-16 March 2013.
Regards, Scott
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X-from: jpshield-at-uga.edu
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Message: Can someone direct me to a good source of literature for sample prep/stain and or contrasting methods for eyes, lizards, other non-murine models for imaging in a MicroCT.
Thanks! John S Univ. of Georgia
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Email: amadden-at-ou.edu Name: Andy Elwood Madden
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Title-Subject: [Filtered] Job Opening for TEM/ Imaging Research Scientist
Message: The Samuel Roberts Noble Microscopy Laboratory (http://www.microscopy.ou.edu) at the University of Oklahoma, Norman Campus (http://www.ou.edu) invites applications for a full-time Research Scientist. The successful applicant will join our team of scientists in an interdisciplinary multi-user microscopy laboratory to advance the research, teaching, and service missions of OU and the broader region. In particular, the candidate will operate, maintain, supervise and provide research-related training principally on transmission electron microscopy and related ancillary equipment in a core light/electron imaging facility. Applicants MUST apply for this position on-line at https://jobs.ou.edu under the job requisition #193037 (Scientist/Researcher II). Applicants should submit (1) a cover letter that includes a description of their experience with both transmission electron microscopy (including sample preparation and various imaging and analysis modes) and working in an interdisciplinary multi-user collaborative environment; (2) a CV that includes a listing of 2-3 references that can provide relevant details regarding the applicant’s suitability for this position. The position is available immediately and will remain open until filled. For further information, please contact Dr. Andy Elwood Madden at amadden-at-ou.edu. The University of Oklahoma (OU) is a Carnegie-R1 comprehensive public research university known for excellence in teaching, research, and community engagement. The 277-acre Research Campus in Norman was named the No.1 research campus in the nation by the Association of Research Parks in 2013. Norman is a culturally rich and vibrant town located just outside Oklahoma City. With outstanding schools, amenities, and a low cost of living, Norman is a perennial contender on the “Best Places to Live” rankings. Visit http://soonerway.ou.edu/ for more information.
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Can anybody give a rough estimate of the relative sizes (compared to the back focal plane) of various diffraction patterns in a typical 200 kV TEM run in imaging mode at about 70000 times magnification? Especially the final diffraction pattern between the projector and the fluorescence screen would be of interest.
All the best,
Philip
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Email: allan.mitchell-at-otago.ac.nz Name: Allan Mitchell
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Title-Subject: [Filtered] Using Leica EMPACT Specimen Tube system with cellulose tubing
Message: Hi, I have a researcher who would like to use our Leica EMPACT 2 with the Specimen Tube system and with specimen loaded cellulose capillary tubing inside the copper tube. I have never used the Specimen Tube system before, all our freezing to date has been using membrane carriers. My question, once you have the specimen in the cellulose capillary tubing and the cellulose tubing inserted into the copper tube, how do you trim and seal the cellulose tubing so that it does not poke out either end of the copper tube? My concern is that if any of the cellulose tubing pokes out of the copper tubing and nto the flange area, it will prevent the copper tube flange from forming a perfect pressure seal therefore prevent us from achieving the required pressure for vitrification. Thanks, Allan Login Host: 139.80.40.139 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
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Only THREE WEEKS to go until our conference!
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Please have a look at the website. Submit a poster. Attend a workshop. Visit Hobbiton!!
Workshops: 1. OME (Open Microscopy Environment) - Imaging Workflows in OMERO 2. Introduction to EBSD and TKD 3. From Image Analysis to the land of Machine Learning
It would be great to see you here if you are able!!
Regards, Helen Turner Conference Convenor
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Hi Allan, I've only used the cellulose capillary tube once or twice with the tube system on the EM-pact, the loading mostly depends on whether you are dealing with a liquid sample (cells in suspension) or with pre-loaded capillaries (cells grown inside the capillary). In the first situation, the empty capillary is fed through the (mounted)tube until it sticks out on the other end and then the sample is drawn in by capillary force. (note that this doesnt't work that well with viscous samples, there you might have to pipette into the capillary). Next the capillary is drawn back into the tube and the other end is cut off next to the tube using a scalpel blade. The tube system is then loaded into the Em-pact with the cut-side facing the bayonet holder. If you are dealing with pre-loaded capillaries, the tubes are cut to fit within the tube. if you use a slightly blunted scalpel for that you actually seal the capillary while cutting. (see also Chapter 28 - “Tips and Tricks” for High-Pressure Freezing of Model Systems, in methods in cell biology) an other method is using heated tweezers to create a seal (Rieger et al. Archives of Microbiology, 1997). Here it is probably best to first fill the tube system with an appropriate buffer/filler and then slide in the capillary to ensure no air is trapped inside the system. Again make sure the capillary doesn't extend from the tube, especially on the side where it makes contact with the pressurizing system. Best Rob
--- Dr. Rob Mesman Post-Doc Department of Microbiology Faculty of Science Radboud University Nijmegen Heyendaalseweg 135 NL-6525 AJ Nijmegen The Netherlands
On 2019-10-22 13:59, microscopy.listserver-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: allan.mitchell-at-otago.ac.nz } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy to both } allan.mitchell-at-otago.ac.nz, Microscopy-at-Microscopy.com so that all } Microscopy Listserver } Subscribers can benefit from our collective wisdom } --------------------------------------------------------------------------- } } Email: allan.mitchell-at-otago.ac.nz Name: Allan Mitchell } } Organization: University of Otago } } Title-Subject: [Filtered] Using Leica EMPACT Specimen Tube system with } cellulose tubing } } Message: Hi, I have a researcher who would like to use our Leica } EMPACT 2 with the Specimen Tube } system and with specimen loaded cellulose capillary tubing inside the } copper tube. I have never } used the Specimen Tube system before, all our freezing to date has } been using membrane carriers. My } question, once you have the specimen in the cellulose capillary tubing } and the cellulose tubing } inserted into the copper tube, how do you trim and seal the cellulose } tubing so that it does not } poke out either end of the copper tube? My concern is that if any of } the cellulose tubing pokes out } of the copper tubing and nto the flange area, it will prevent the } copper tube flange from forming a } perfect pressure seal therefore prevent us from achieving the required } pressure for vitrification. } Thanks, Allan } Login Host: 139.80.40.139 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original } Headers============================== } 8, 53 -- From microscopy.listserver-at-gmail.com Tue Oct 22 06:51:36 2019 } 8, 53 -- Received: from mail-il1-f179.google.com } (mail-il1-f179.google.com [209.85.166.179]) } 8, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } x9MBpaTZ026587 } 8, 53 -- for {microscopy-at-microscopy.com} ; Tue, 22 Oct 2019 06:51:36 } -0500 } 8, 53 -- Received: by mail-il1-f179.google.com with SMTP id } l12so15132246ilq.4 } 8, 53 -- for {microscopy-at-microscopy.com} ; Tue, 22 Oct 2019 } 04:59:00 -0700 (PDT) } 8, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 8, 53 -- d=gmail.com; s=20161025; } 8, 53 -- } h=subject:references:to:from:message-id:date:user-agent:mime-version } 8, 53 -- } :in-reply-to:content-language:content-transfer-encoding; } 8, 53 -- bh=xsW7ZZTatWLnRnDgwRKpXiOwoActbj4JotXjuu1LgCU=; } 8, 53 -- } b=Xgul+kqiaQhhSJMvESS5I/frEvrqtk2RqK1U5obLyfrgjiLrT5DUVOrJparSH59YNJ } 8, 53 -- } Il12gjqLc8HXuqOXJsn6YA2Yqn2pXKQLPbuLl2DbpvfW8c0pLXaJozof41YwSesaDyJn } 8, 53 -- } mMdzNmiKhqiWWmf7MOtbDPhloMx8SJecfBwzyr1DbHovzQwYCS6/UP02UGYgRZrz0lqZ } 8, 53 -- } q6ojp+7QMmnWKOWvmgohpBKx2cf39Wpxv3QnaqalB5mLdKicUhZzzyZhwVeiEXFQmiyR } 8, 53 -- } Ci/AtFZ60jGCI0N9mVxfwOHS32wvSrFHyFdNcEGe0KS/tf2bZk/mr/TNmYSQZa9ZsMBa } 8, 53 -- YXfw== } 8, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 8, 53 -- d=1e100.net; s=20161025; } 8, 53 -- } h=x-gm-message-state:subject:references:to:from:message-id:date } 8, 53 -- :user-agent:mime-version:in-reply-to:content-language } 8, 53 -- :content-transfer-encoding; } 8, 53 -- bh=xsW7ZZTatWLnRnDgwRKpXiOwoActbj4JotXjuu1LgCU=; } 8, 53 -- } b=j9V+4BfLgvdjDblgBBxvAqWKwiJ7FSXmB4s59YB7xaKM7uARKQPTr3b4PPBYYZkPNf } 8, 53 -- } q5XqJs1EhVoS43INDDEL0krSqbtslaMqy1+o49JK8yZ8Zq10KefgO7QpM0DQ+GVOl/5B } 8, 53 -- } lq0xKVig/HE2FJstsQu0Nez3/r7Eh1Au7TGS/IU82eHMHJ0ktoLBL84ol4pRAZekvjcI } 8, 53 -- } otML8oHzFAQx2XyvGiLD5nSCMjlViKDITtIeVyfWWfNXM39ya58Gw7KTzh2681W5lu0c } 8, 53 -- } yMHWhQhO+AE20LC2gY5ffoDWhqiMW1LrGkZcIfdHR51AbpcCVqJZP4Gc7Pcwbxt8uzs0 } 8, 53 -- J5BQ== } 8, 53 -- X-Gm-Message-State: } APjAAAUdmDfCE9s6bPcorF2mungN6yq0Cbo49ewwYYlaIovPqLiLI90V } 8, 53 -- ufib0ge0daql+hwcUBxJz+UvlJIl } 8, 53 -- X-Google-Smtp-Source: } APXvYqy+ioqoyDvexVxPo9OCm1+DGQ1LXUtrDwolfe5d4DU2qtMviBsUCJqtGUEd8SIsa4JEMmOxQg== } 8, 53 -- X-Received: by 2002:a92:98d8:: with SMTP id } a85mr30209363ill.98.1571745539571; } 8, 53 -- Tue, 22 Oct 2019 04:58:59 -0700 (PDT) } 8, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net } ([2603:300a:f04:7100:95d3:33bd:1278:6ce6]) } 8, 53 -- by smtp.googlemail.com with ESMTPSA id } o26sm5278692ioo.61.2019.10.22.04.58.57 } 8, 53 -- for {microscopy-at-microscopy.com} } 8, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 } bits=128/128); } 8, 53 -- Tue, 22 Oct 2019 04:58:58 -0700 (PDT) } 8, 53 -- Subject: viaWWW:Using Leica EMPACT Specimen Tube system with } cellulose tubing } 8, 53 -- References: {201910220152.x9M1qNKD004129-at-microscopy.com} } 8, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 8, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 8, 53 -- X-Forwarded-Message-Id: } {201910220152.x9M1qNKD004129-at-microscopy.com} } 8, 53 -- Message-ID: {9b81fae1-298f-30d6-cdd3-accaddbb22f5-at-gmail.com} } 8, 53 -- Date: Tue, 22 Oct 2019 06:58:57 -0500 } 8, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.14; } rv:60.0) } 8, 53 -- Gecko/20100101 Thunderbird/60.9.0 } 8, 53 -- MIME-Version: 1.0 } 8, 53 -- In-Reply-To: {201910220152.x9M1qNKD004129-at-microscopy.com} } 8, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 8, 53 -- Content-Language: en-US } 8, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - } Headers==============================
==============================Original Headers============================== 4, 26 -- From R.Mesman-at-science.ru.nl Tue Oct 22 09:02:02 2019 4, 26 -- Received: from smtp3.science.ru.nl (smtp3.science.ru.nl [131.174.30.193]) 4, 26 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x9ME21Vr012159 4, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 22 Oct 2019 09:02:02 -0500 4, 26 -- Received: from roundcube.science.ru.nl (weber.science.ru.nl [131.174.30.199]) 4, 26 -- by smtp3.science.ru.nl (8.15.2/5.32) with ESMTP id x9ME9O0k024607; 4, 26 -- Tue, 22 Oct 2019 16:09:24 +0200 4, 26 -- Received: from n018157.science.ru.nl ([131.174.18.157]) 4, 26 -- by roundcube.science.ru.nl 4, 26 -- with HTTP (HTTP/1.1 POST); Tue, 22 Oct 2019 16:09:24 +0200 4, 26 -- MIME-Version: 1.0 4, 26 -- Content-Type: text/plain; charset=UTF-8; 4, 26 -- format=flowed 4, 26 -- Content-Transfer-Encoding: 8bit 4, 26 -- Date: Tue, 22 Oct 2019 16:09:24 +0200 4, 26 -- From: Rob Mesman {R.Mesman-at-science.ru.nl} 4, 26 -- To: Microscopy-at-microscopy.com, allan.mitchell-at-otago.ac.nz 4, 26 -- Subject: Re: [Microscopy] viaWWW:Using Leica EMPACT Specimen Tube system with 4, 26 -- cellulose tubing 4, 26 -- In-Reply-To: {201910221159.x9MBxnqK004229-at-microscopy.com} 4, 26 -- References: {201910221159.x9MBxnqK004229-at-microscopy.com} 4, 26 -- Message-ID: {d4ca1554acf03d2022cf32cc4c06d220-at-science.ru.nl} 4, 26 -- X-Sender: R.Mesman-at-science.ru.nl 4, 26 -- User-Agent: Roundcube Webmail/1.3.6 4, 26 -- X-Spam-Score: 1 (*) ALL_TRUSTED,BAYES_50 4, 26 -- X-Scanned-By: mimedefang version 2.83 on 131.174.30.193 131.174.30.60 (smtp3) ==============================End of - Headers==============================
I'd like to change the focus of my question. In normal imaging mode (70 000 mag) is there somewhere in the column a diffraction pattern that is bigger than the one in the BFP of the OL? If so, where is it and how much bigger is it roughly?
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Email: zzhang-at-uwyo.edu Name: Zhaojie Zhang
Organization: University of Wyoming
Title-Subject: [Filtered] electron microscopy lab manager at the University of Wyoming
Message: The Center of Innovation for Flow Through Porous Media (COIFPM) at the University of Wyoming is seeking a lab manager for its electron microscopy lab. You can find more about COIFPM at its website - https://coifpm.com/. Qualification and requirement for this position can be found at - https://uwyo.taleo.net/careersection/00_ex/jobdetail.ftl?job=19004175&tz=GMT-06%3A00&tzname=America%2FDenver
Questions should be sent to:
Dr. Lamia Goual Associate Professor A. J. Castagne Professor in the College of Engineering and Applied Science University of Wyoming Email: mailto:lgoual-at-uwyo.edu
---------------------------------------- Zhaojie Zhang, Ph.D. Director, Jenkins Microscopy Facility University of Wyoming PHONE: 307-766-3038 ---------------------------------------------
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Email: glaevsky-at-princeton.edu Name: Gary S Laevsky
Organization: Princeton University
Title-Subject: [Filtered] North Atlantic Microscopy Society NAMS Annual Meeting November 1, 2019
Message: Hello All,
We are very excited to announce our second annual meeting!
Our plenary speakers for this meeting will be;
Jeremy Barry from GlaxoSmithLine to speak about Mass Spectometry Imaging
Ed Eng from The New York Structural Biology Center will be our Cryo-EM spea= ker
And Clare Waterman, an NIH Distinguished Investigator, will be our Light Mi= croscopy speaker.
Anyone the presents a poster can attend for free.
Nikon has generously offered a $100 travel award to a lucky participant!
There will be approximately 25 vendors present.
namsmicroscopy (dot) com/meeting-registration for details
Gary Laevsky Co-founder Paul Shao Co-founder Shawn Davidson Co-organizer
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X-from: Ray Twesten {RTwesten-at-gatan.com} Philip,
This is given by: (see {http://eels.info/products/spectroscopy-operation} ) You can derive this using just assuming a single thin lens between the sample and the screen, the known magnification and the distance from the projector crossover to the screen.
TEM imaging mode With the TEM in the Imaging mode, an image is formed on the viewing screen, while the projector crossover contains a small diffraction pattern. Because the spectrometer projects an energy dispersed diffraction pattern onto the detector, this mode is also known as diffractionâ€coupled. The camera length L of the pattern in the projector crossover is given by
L=hM and the diameter of the projector crossover, dp, is dp=2ĂźhM
where h = distance from the projector crossover to the viewing screen M = image magnification at the viewing screen Ăź = half acceptance angle as defined by the objective aperture
The value of h depends on the TEM. It is about 325 to 390 mm. It is further to the film plane or the camera below the film.
Ray D. Twesten, Ph.D. Product Manager – Analytical Instruments  Gatan, Inc.  Tel. +1 (925) 224-7392
This message and any attachments are solely for the use of intended recipients. They may contain privileged and/or confidential information.
-----Original Message----- X-from: philip.koeck-at-ki.se {philip.koeck-at-ki.se} Sent: Tuesday, October 22, 2019 1:57 AM To: Ray Twesten {RTwesten-at-gatan.com}
Hi.
Can anybody give a rough estimate of the relative sizes (compared to the back focal plane) of various diffraction patterns in a typical 200 kV TEM run in imaging mode at about 70000 times magnification? Especially the final diffraction pattern between the projector and the fluorescence screen would be of interest.
All the best,
Philip
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Email: susan.vanhorm-at-stonybrook.edu Name: Susan Van Horn
Organization: Stony Brook University
Title-Subject: [Filtered] Uranyl Acetate
Message: We have been ordering our UA from SPI Supplies ( Structure Probe, Inc) - all of a sudden we cannot get our usual concentration 0,3gm/15ml methanol or aqueous into solution.....it remains cloudy after 30mins of stirring.....we had this problem with EMS UA years ago and switched to SPI - now we are having same problem......we run it through a filter and it seems to stain ok.....was just wondering if anyone has had this problem....it is a different Lot# but of course the company said nothing has changed thanks Sue
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Title-Subject: [Filtered] Research Fellow position in TEM of Functional Optoelectronic Interfaces at Monash University
Message: Applications for a two-year research fellowship on TEM of functional optoelectronic interfaces are now open -- see http://careers.pageuppeople.com/513/cw/en/job/598514/research-fellow-hrtem-of-functional-optoelectronic-interfaces
The microscopy will take place within the Monash Centre for Electron Microscopy at Monash University, Australia.
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==============================Original Headers============================== 10, 54 -- From microscopy.listserver-at-gmail.com Fri Oct 25 07:31:28 2019 10, 54 -- Received: from mail-io1-f52.google.com (mail-io1-f52.google.com [209.85.166.52]) 10, 54 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x9PCVSce015744 10, 54 -- for {microscopy-at-microscopy.com} ; Fri, 25 Oct 2019 07:31:28 -0500 10, 54 -- Received: by mail-io1-f52.google.com with SMTP id p6so2213454iod.7 10, 54 -- for {microscopy-at-microscopy.com} ; Fri, 25 Oct 2019 05:39:02 -0700 (PDT) 10, 54 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 10, 54 -- d=gmail.com; s=20161025; 10, 54 -- h=subject:references:to:from:message-id:date:user-agent:mime-version 10, 54 -- :in-reply-to:content-language:content-transfer-encoding; 10, 54 -- bh=G8HTk50ea27hetY4RWrw1XXHJGM6RSNoprkmn0rmm0c=; 10, 54 -- b=SXbwSp8BUnlSdMswaKDUWXX9srt/pyt1dzXZbc3J7n6DrE3yDTyswMI+R103daFqhS 10, 54 -- hVt/Q/H9w/0WcrwNGiWBrMGDwhw0Z4jAD5d+1oKzp2rMHuQqiqXi2a46mJDO4NhlLcC5 10, 54 -- n/5pwGu30Vv/mK4ZAzN9Lm6GgaiBto3e1KqUB4pgTO80H1dIENGBg6EeVMeCQ6IF64lo 10, 54 -- YZl/eauMLXlcfUthtkqbQ7o6Q9OMdDCQL5/eCSEViKcbn6EAROJXKq05V7p2T6GOUy0H 10, 54 -- nioZV85ZWK+3td/myG8tvwUIoLZeXSMseBm4IMteK7cieEqOUdrzhh0dkF8pITKR74J1 10, 54 -- QlAQ== 10, 54 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 10, 54 -- d=1e100.net; s=20161025; 10, 54 -- h=x-gm-message-state:subject:references:to:from:message-id:date 10, 54 -- :user-agent:mime-version:in-reply-to:content-language 10, 54 -- :content-transfer-encoding; 10, 54 -- bh=G8HTk50ea27hetY4RWrw1XXHJGM6RSNoprkmn0rmm0c=; 10, 54 -- b=mHca6PqPml7DsefviS4gKT4aWCcjhHMZmvsZKQ0oGjNLQAcq1LUgrRX4W6zv6FTkxa 10, 54 -- 5+SaZ12sPsXfz2lwiZHWVIvITU7iNHcUyCmWBBLZWroJ5102xJ2JgseqlWFJrzgXJ/RH 10, 54 -- 2Bd5MMh6WhdTlyITpDkI88py3zfEMK5tXX87xOKiCVp92dDlPDMcZBmOeESNZhfaGydw 10, 54 -- WFECO68DjdntfBUff7LFzDY9jxjnFmF/HNVVlkpm0KMgTT+DXikv1FXK328MFrS3VI3W 10, 54 -- I6N/AAjSJ0smutZhgTEC/rdsjNZovVHrVyG8JEeVzfEwNIk20zePpdSAsO3DjdhUtD0K 10, 54 -- h45w== 10, 54 -- X-Gm-Message-State: APjAAAVBzoGc5U5nPFqEZY/QgdGshWkzMNtQrBmS4WlI/ERWVkZfkdQh 10, 54 -- L+Gbi4T05jXj5vLOrWNK0rZZCFtn 10, 54 -- X-Google-Smtp-Source: APXvYqyqOVzTqI+swX8q3OZ9dvtZ9ZFQwM0uvCNSdErWU6y/2cx1Qw/XSHekROhaxFNFZAe5CHqYrA== 10, 54 -- X-Received: by 2002:a5d:928a:: with SMTP id s10mr3368441iom.10.1572007141964; 10, 54 -- Fri, 25 Oct 2019 05:39:01 -0700 (PDT) 10, 54 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:2986:4136:ac81:e532]) 10, 54 -- by smtp.googlemail.com with ESMTPSA id z1sm261127ioe.8.2019.10.25.05.39.01 10, 54 -- for {microscopy-at-microscopy.com} 10, 54 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 10, 54 -- Fri, 25 Oct 2019 05:39:01 -0700 (PDT) 10, 54 -- Subject: viaWWW: Research Fellow position in TEM of Functional Optoelectronic 10, 54 -- Interfaces at Monash University 10, 54 -- References: {201910250227.x9P2RiJf030013-at-microscopy.com} 10, 54 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 10, 54 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} 10, 54 -- X-Forwarded-Message-Id: {201910250227.x9P2RiJf030013-at-microscopy.com} 10, 54 -- Message-ID: {c13ccdc9-107b-0b94-f0d8-c9a2a4001df4-at-gmail.com} 10, 54 -- Date: Fri, 25 Oct 2019 07:39:01 -0500 10, 54 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.14; rv:60.0) 10, 54 -- Gecko/20100101 Thunderbird/60.9.0 10, 54 -- MIME-Version: 1.0 10, 54 -- In-Reply-To: {201910250227.x9P2RiJf030013-at-microscopy.com} 10, 54 -- Content-Type: text/plain; charset=windows-1252; format=flowed 10, 54 -- Content-Language: en-US 10, 54 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Hi Susan, With a good batch of uranyl acetate you should be able to dissolve up to 20% of UA in methanol I've had the same problem a few years ago with a batch of SPI uranyl acetate, it was impossible to dissolve in anything (spent a few weeks trying all the tricks I could find). After a lot of e-mailing I could send the bottle back to SPI. Since then I've been relying on uranyl acetate from Agar Scientific (availaible via Ted Pella in the US) for post-staining and a +/- 25 year old bottle of Merck uranyl acetate for freeze-substitution. The main problem is that there are apparently only one or two sources of uranyl acetate which are used by all suppliers, every now and then a bad batch ends up somewhere. You can ask the supplier if the lot they're currently selling has been tested for solubility (a few suppliers actually test this). Best,
Rob
--- Dr. Rob Mesman Post-Doc Department of Microbiology Faculty of Science Radboud University Nijmegen Heyendaalseweg 135 NL-6525 AJ Nijmegen The Netherlands
On 2019-10-25 14:40, microscopy.listserver-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: susan.vanhorm-at-stonybrook.edu } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy to both } susan.vanhorm-at-stonybrook.edu, Microscopy-at-Microscopy.com so that all } Microscopy Listserver } Subscribers can benefit from our collective wisdom } --------------------------------------------------------------------------- } } Email: susan.vanhorm-at-stonybrook.edu Name: Susan Van Horn } } Organization: Stony Brook University } } Title-Subject: [Filtered] Uranyl Acetate } } Message: We have been ordering our UA from SPI Supplies ( Structure } Probe, Inc) - all of a sudden we } cannot get our usual concentration 0,3gm/15ml methanol or aqueous into } solution.....it remains } cloudy after 30mins of stirring.....we had this problem with EMS UA } years ago and switched to SPI - } now we are having same problem......we run it through a filter and it } seems to stain ok.....was just } wondering if anyone has had this problem....it is a different Lot# but } of course the company said } nothing has changed } thanks } Sue } } Login Host: 129.49.104.239 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original } Headers============================== } 9, 53 -- From microscopy.listserver-at-gmail.com Fri Oct 25 07:30:50 2019 } 9, 53 -- Received: from mail-io1-f43.google.com } (mail-io1-f43.google.com [209.85.166.43]) } 9, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } x9PCUo3n015167 } 9, 53 -- for {microscopy-at-microscopy.com} ; 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} 9, 53 -- Fri, 25 Oct 2019 05:38:23 -0700 (PDT) } 9, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net } ([2603:300a:f04:7100:2986:4136:ac81:e532]) } 9, 53 -- by smtp.googlemail.com with ESMTPSA id } g23sm257714ioe.73.2019.10.25.05.38.22 } 9, 53 -- for {microscopy-at-microscopy.com} } 9, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 } bits=128/128); } 9, 53 -- Fri, 25 Oct 2019 05:38:23 -0700 (PDT) } 9, 53 -- Subject: viaWWW:Uranyl Acetate } 9, 53 -- References: {201910241711.x9OHBqMH012592-at-microscopy.com} } 9, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 9, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 9, 53 -- X-Forwarded-Message-Id: } {201910241711.x9OHBqMH012592-at-microscopy.com} } 9, 53 -- Message-ID: {11823577-e87e-299d-d147-0ab7534e0ff3-at-gmail.com} } 9, 53 -- Date: Fri, 25 Oct 2019 07:38:22 -0500 } 9, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.14; } rv:60.0) } 9, 53 -- Gecko/20100101 Thunderbird/60.9.0 } 9, 53 -- MIME-Version: 1.0 } 9, 53 -- In-Reply-To: {201910241711.x9OHBqMH012592-at-microscopy.com} } 9, 53 -- Content-Type: text/plain; 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==============================Original Headers============================== 4, 25 -- From R.Mesman-at-science.ru.nl Fri Oct 25 08:15:44 2019 4, 25 -- Received: from smtp3.science.ru.nl (smtp3.science.ru.nl [131.174.30.193]) 4, 25 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x9PDFhI5030617 4, 25 -- for {Microscopy-at-Microscopy.com} ; Fri, 25 Oct 2019 08:15:44 -0500 4, 25 -- Received: from roundcube.science.ru.nl (weber.science.ru.nl [131.174.30.199]) 4, 25 -- by smtp3.science.ru.nl (8.15.2/5.32) with ESMTP id x9PDNGJZ008870; 4, 25 -- Fri, 25 Oct 2019 15:23:16 +0200 4, 25 -- Received: from n018157.science.ru.nl ([131.174.18.157]) 4, 25 -- by roundcube.science.ru.nl 4, 25 -- with HTTP (HTTP/1.1 POST); Fri, 25 Oct 2019 15:23:16 +0200 4, 25 -- MIME-Version: 1.0 4, 25 -- Content-Type: text/plain; charset=US-ASCII; 4, 25 -- format=flowed 4, 25 -- Content-Transfer-Encoding: 7bit 4, 25 -- Date: Fri, 25 Oct 2019 15:23:16 +0200 4, 25 -- From: Rob Mesman {R.Mesman-at-science.ru.nl} 4, 25 -- To: Microscopy-at-Microscopy.com, susan.vanhorm-at-stonybrook.edu 4, 25 -- Subject: Re: Uranyl Acetate 4, 25 -- In-Reply-To: {201910251240.x9PCeRoY029209-at-microscopy.com} 4, 25 -- References: {201910251240.x9PCeRoY029209-at-microscopy.com} 4, 25 -- Message-ID: {bdb4183fdce3d98e3858b10f79f283fb-at-science.ru.nl} 4, 25 -- X-Sender: R.Mesman-at-science.ru.nl 4, 25 -- User-Agent: Roundcube Webmail/1.3.6 4, 25 -- X-Spam-Score: 1 (*) ALL_TRUSTED,BAYES_50 4, 25 -- X-Scanned-By: mimedefang version 2.83 on 131.174.30.193 131.174.30.60 (smtp3) ==============================End of - Headers==============================
From sawijama5vydy-at-gmail.com Sat Oct 26 09:23:56 2019 Return-Path: {sawijama5vydy-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.162.221] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x9QENphH003543 for {microscopylistserverarchive7-at-microscopy.com} ; Sat, 26 Oct 2019 09:23:55 -0500 Message-ID: {FDC270A7.25841F6F-at-gmail.com}
Hello All,
Friendly reminder to register for the upcoming meeting Nov. 1, 2019 at Princeton University.
Come see and hear what people in the neighboring "silo" are doing.
We are very excited about our plenary speakers for this annual meeting; Jeremy Barry from GlaxoSmithKline will talk about Mass Spectometry Imaging Ed Eng from the New York Structural Biology Center will be our Cry-EM speaker And Clare Waterman, an NIH Distinguished Investigator, will be our Light Microscopy speaker.
Anyone that presents a poster can attend free (abstract submitted with application).
Nikon has also generously offered a $100 travel award to a lucky participant!
There will be approximately 25 vendors present!
namsmicroscopy.com/meeting-registration
Final Meeting Schedule
8a                        Registration/Breakfast         Convocation Room 9a-9:30a               Opening Remarks                                     Laevsky/Shao                                     Sabine Petry-Assistant Professor Molecular Biology                                     Craig Arnold• Director, Princeton Institute for the Science and Technology of Materials                                     Pablo Debenedetti -  Dean for Research              9:30a -10:15        Jeremy Barry (GlaxoSmithKline)•Seeing Is Bellevlng: The impact of Imaging Mass Spectromeny In drug discovery & development 10:15a-11:15       Tech Bite Talks 11:30a-l2:15        LightningTalks                                     Dongfang Liu                                    Arek Kulczyk                                           Matthias Kock 12:15p-2p           Lunch/Vendor vislts/Posters 2p-3p                  Ed Eng(New YorkStructural Biology Center) .. What to expect from cryo•EM {national service centers} 3p-4p                  Clare Waterman (NIH Distinguished Investigator) 4p-4:15p             Coffee Braek 4:15p-5:30p        Panel digussions                                     Big Data                                     Mass Spec Imaging                                     Cryo EM                                    Super Resolution - When, where,and whv
5:30Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Pizza and beverages
==============================Original Headers============================== 12, 37 -- From gary_laevsky-at-yahoo.com Mon Oct 28 07:16:33 2019 12, 37 -- Received: from sonic312-20.consmr.mail.bf2.yahoo.com (sonic312-20.consmr.mail.bf2.yahoo.com [74.6.128.82]) 12, 37 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x9SCGWq9029758 12, 37 -- for {microscopy-at-microscopy.com} ; Mon, 28 Oct 2019 07:16:32 -0500 12, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1572265456; bh=itop1ZlQb5dQpeQZ/OheHnpKvB+XbsYdgcLg9Wcymvw=; h=Date:From:To:Subject:References:From:Subject; b=I5r1pSHxYJ1QqlbxuYTnPBdq0c9fMJZqlsDDVA+kXApchbg2fmjCvO96A19m/QDe7pzxf9CglYq1dvcAwv90Pew2Pam29BOGCsCLQWSTB1vIQHcGyeXNLogkZGnLKm+9unhofxon8DwLZv8ns0U5IMCtI2WtEZ+27yx/cj4MSbKQbkf6AGANJ4IysuMHhXbZTfrpZ0rj4DIvOQRmzMcP57N6zkg3KHqDxGmB/zPiCdC4JrDOq0c46swMiE66HKAr6Ued/oG8USTmaXNsZmidgbOvMoWrRpb/15253Pyqf30NI2sts55k8t931z1LnXQxQfLGH1/vP1B95hzcAatGeg== 12, 37 -- X-YMail-OSG: ijQ6HcsVM1nNPOmr4mjcbIK5FT39SWt4m_bYS2ti06Jhe00cYqzMm1E4cj94y8x 12, 37 -- pKRu0fufUKJMk5n.Hk7GQqU9OdC5E2DayiHm58PxfM1aHw_jWEtFJRNXVfBXbgWf507nh5Fsr_40 12, 37 -- Z0yRhMAiazYt4xce.gNpDNKF1xqXyiG3yxCLtxQWIXklQ06UsX0wEI_rDmlBGs3LagIldPTellbP 12, 37 -- HuSWOvS76f1Akpi5sSsCt7yVDOqgftskL.QDH9WfLHQw3rQ7A0MmtY5OUWhCKYjnlpi_5wlUfOvc 12, 37 -- g0PshM2N4KBGFjJnPbGJfDaN1F1O1s4Npf4AOm_JX0lDOAbSSL1FiPh.tIgwD3j9TtCzy_Sfmno8 12, 37 -- XgdCwCvCw2k6j9hPfnBQS3UCr_tQ4jSASzwndKcyTuSNPWFlEkE6GtJ5Qk2wKsVhnqINyOJEUtnh 12, 37 -- k3cGkuTbEi8cbzc0AB3Zq0yims9jArK6l_6FAo2h2TdhlJ1yrdLItHkRrnnLiQjdXldCCLxNr4vh 12, 37 -- tASurB08fuqFGhYrWmsI03SrgS6689ktGd6XctfCkZakmzovvOdlAj.xfgJTwWNxplcLJA9AeGDy 12, 37 -- evq3cYsXeb5bH_C8Qm11YdWOUWDp74QPY2XoCfFs4DTWKGaIXjxSyYzpZSmtlRCVvfyAFB_AOc0i 12, 37 -- m9Qez6NkIKulRmyJ6vt1cUrY7NimWtqh3FEdh0h.8TVCrpoYKm9AV.FwOsRaESsRZYtX1qpZ9UTb 12, 37 -- XKnOfBgc0qZJ_6HZ1oApUM9rcPJrTshC50Y.ts0wc3mRCbiG7wBDWCIl.ykm0rwDXw.F2uKT2b7a 12, 37 -- BX5exqb9Zxo8JpWoc6Ynizex7GQ6EDhKdB3nEnw3_Y71gTgOFhvw9M.juNSbV0aVrGfXAIpG8aq4 12, 37 -- CN6eTZIHbmj9iAwuVhCY4yim.hq9LkeRB3nCguoVGA9zj44X9YWKlTfYbuMuV0fd81K5A9C3ULhC 12, 37 -- p6Yv616VZDS6BB7Xn6lLSPhUACyMZ9fnClEnLUBamh87_VKgxF8uu14cF1RHhgSfLLoRw7SZy5VK 12, 37 -- wUJSXzjSvSmESmq.cff5iL_XXTod9hBeFRYtyINLRIvzdy2TumgBiTiTT.XyHQu8NjY7cu68ZfJd 12, 37 -- 3zBfK4bECPsa7GrOXOk_t6RZRBuQxVVtQl5vR22cfixHEdxGRySUY5LyeCwq1f2FgYjTCZvs2QWh 12, 37 -- CppykXxKKshTnGRJLdte16p8aqDxiz.A288hygEL4o9V1Lss.DjpfpKFUwAlZAzDi931QJGtQp80 12, 37 -- lLjE158xC1BSlTYxLNd9ot4zOnahtBVzPVixfIGH4TWjgsBXd4y5pmxv8PM1lbe4rbPKAVD2Ck4G 12, 37 -- F_5L8aYDP3wXuQ4NZ8FANPc.h 12, 37 -- Received: from sonic.gate.mail.ne1.yahoo.com by sonic312.consmr.mail.bf2.yahoo.com with HTTP; Mon, 28 Oct 2019 12:24:16 +0000 12, 37 -- Date: Mon, 28 Oct 2019 12:24:14 +0000 (UTC) 12, 37 -- From: Gary Laevsky {gary_laevsky-at-yahoo.com} 12, 37 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 12, 37 -- Message-ID: {422429419.1269578.1572265454925-at-mail.yahoo.com} 12, 37 -- Subject: Last Call!: North Atlantic Microscopy Society (NAMS) Annual Meeting 12, 37 -- at Princeton University Nov. 1, 2019 12, 37 -- MIME-Version: 1.0 12, 37 -- Content-Type: text/plain; charset=UTF-8 12, 37 -- References: {422429419.1269578.1572265454925.ref-at-mail.yahoo.com} 12, 37 -- X-Mailer: WebService/1.1.14593 YMailNorrin Mozilla/5.0 (Macintosh; Intel Mac OS X 10.14; rv:69.0) Gecko/20100101 Firefox/69.0 12, 37 -- Content-Transfer-Encoding: 8bit 12, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id x9SCGWq9029758 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both sergey-at-seas.ucla.edu, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Message: Dear Fellow Microscopists: This is to inform you that Southern California Society for Microscopy and Microanalysis (SCSMM) 2019 fall meeting is scheduled for Wednesday, November6, 2019 at Beckman Institute Auditorium, Caltech. The meeting starts with a happy hour at 5:30 PM followed by dinner and talks.
We are excited to have Dr. Will Harris (KMLabs Inc.) as our invited keynote speaker. The title of his talk is: “Small and Fast: Probing the Intersection of Nanoscale Structure and Ultrafast Dynamics with Lab-Based EUV Laser Light”. Please sign up here: https://imri.uci.edu/content/2019-fall-meeting-registration
We look forward to seeing you all at SCSMM fall meeting, 2019. You can find our NewsLetter and more details here: www.scsmm.org
Sincerely, SCSMM board
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It has been a very long time since I have had to use tungsten wire filaments. Looking for a vendor to rebuild a bunch for a CT instrument. Experiences and suggestions solicited.
Thanks,
Scott Whittaker Lab Manager Scientific Imaging P.O. Box 37012 MRC-104 Room W150 Washington, DC 20013-7012 w 202.633.0891 whittaks-at-si.edu
SMITHSONIAN INSTITUTION NATIONAL MUSEUM OF NATURAL HISTORY Facebook | Twitter | Instagram
-----Original Message----- X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Tuesday, October 29, 2019 7:39 AM To: sales-at-laddresearch.com
X-from: Whittaker, Scott {WHITTAKS-at-si.edu}
All,
It has been a very long time since I have had to use tungsten wire filaments. Looking for a vendor to rebuild a bunch for a CT instrument. Experiences and suggestions solicited.
Thanks,
Scott Whittaker Lab Manager Scientific Imaging P.O. Box 37012 MRC-104 Room W150 Washington, DC 20013-7012 w 202.633.0891 whittaks-at-si.edu
SMITHSONIAN INSTITUTION NATIONAL MUSEUM OF NATURAL HISTORY Facebook | Twitter | Instagram
From koepketemi17xothw-at-gmail.com Tue Oct 29 20:47:51 2019 Return-Path: {koepketemi17xothw-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.162.211] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id x9U1lo2P003474 for {microscopylistserverarchive7-at-microscopy.com} ; Tue, 29 Oct 2019 20:47:50 -0500 Message-ID: {EEC706A2.B5B87A83-at-gmail.com}
X-from: plarson-at-ou.edu
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both plarson-at-ou.edu, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Email: plarson-at-ou.edu Name: Preston Larson
Organization: University of Oklahoma
Title-Subject: [Filtered] Zeiss DSM-960A Available
Message: Hi Everyone,
We are decommissioning a Zeiss DSM-960A SEM here at the University of Oklahoma.
When it was shut down, the microscope was operational but it has had extensive work done on it through the years so it might not be the most attractive option to set up as a functional SEM in a lab setting but it would be great as a parts machine if anyone is trying to maintain another Zeiss 960.
If anybody is interested please let us know immediately as we are trying to reclaim lab space as soon as possible. Thanks,
Preston Larson Noble Microscopy Lab University of Oklahoma (405)-325-4391
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both alexandergreene-at-sbcglobal.net, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Email: alexandergreene-at-sbcglobal.net Name: Alex Greene
Organization: Scientific Instrumentation Services, Inc.
Title-Subject: [Filtered] Job Offering
Message: Scientific Instrumentation Services is looking for a part-time Electron Microscope Service Engineer. This can possibly turn into a full-time position. The job is to maintain and verify system operational quality, instruct customers in operation and maintenance of their electron microscope.
What does an FSE do? * Ensure proper working order of Transmission Electron Microscopes and occasionally install or remove the instruments. * Conduct planned preventative maintenance on site. * Provide remote troubleshooting and support for customers. * Develop a positive relationship with TEM users. * Travel, often on short notice, to sites to solve problems and make repairs. * Complete detailed field service reports and keep a log of repair solutions.
Education - Associate, bachelors or masters in hard sciences, engineering or equivalent combination of education and experience in electronic and vacuum system principal and troubleshooting. Experience - Five years or more of hands-on experience installing, troubleshooting, repairing and calibrating complex TEM and SEM systems.
Required Skills and Knowledge: * Ability to work effectively alone and with clientele. * Excellent communication skills; must have a professional working proficiency in English. *Proven ability to troubleshoot complex electromechanical systems to a component level. * Knowledgeable in MS DOS, Windows software and willingness to learn more esoteric operational systems. * Able to read and interpret schematic diagrams and have a mechanical inclination.
Additional *Must be able to obtain a valid passport and travel regionally and internationally as needed. * Valid drivers license required. * Physical requirements include being able to lift up to 50 pounds.
Scientific Instrumentation Services, Inc, is a sustaining member of the Microscopy Society of America. We are dedicated to provide service and support to users and owners of electron microscopes.We are based in Austin, Texas and have been in the business of electron microscope repair, refurbishment and resale since the early 1990s.
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recently I got an electron beam system which consists of an electron source (gun) with a power supply from Bertan, model no. 2493A.
I looked for more information about such a system on the internet, but unfortunately couldn't find any manual, etc. However, I got some other details, for instance that the system was part of an "Electroscan ESEM2020" (with LaB6 cathode).
Could anyone provide me something like a manual for this ESEM2020 or Bertan power supply? Particulary I'm interested in what the setting "KBF / K / KF" on the front panel could mean.
Thanks for any help!
Best regards Robert
==============================Original Headers============================== 10, 25 -- From rjaeger-at-ikp.tu-darmstadt.de Thu Oct 31 10:53:38 2019 10, 25 -- Received: from mail-relay230.hrz.tu-darmstadt.de (mail-relay230.hrz.tu-darmstadt.de [130.83.156.230]) 10, 25 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id x9VFrcLb017240 10, 25 -- for {Microscopy-at-Microscopy.Com} ; Thu, 31 Oct 2019 10:53:38 -0500 10, 25 -- Received: from linix.ikp.physik.tu-darmstadt.de (linix.ikp.physik.tu-darmstadt.de [130.83.133.23]) 10, 25 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 10, 25 -- (Client CN "linix.ikp.physik.tu-darmstadt.de", Issuer "DFN-Verein Global Issuing CA" (verified OK)) 10, 25 -- by mail-relay230.hrz.tu-darmstadt.de (Postfix) with ESMTPS id 473qnS23kHz43rF 10, 25 -- for {Microscopy-at-Microscopy.Com} ; Thu, 31 Oct 2019 17:01:32 +0100 (CET) 10, 25 -- Received: from groupware (groupware.ikp.physik.tu-darmstadt.de [192.168.1.210]) 10, 25 -- by linix.ikp.physik.tu-darmstadt.de (Postfix) with ESMTP id 7E63B1CCFD5 10, 25 -- for {Microscopy-at-Microscopy.Com} ; Thu, 31 Oct 2019 17:01:29 +0100 (CET) 10, 25 -- Date: Thu, 31 Oct 2019 17:01:29 +0100 10, 25 -- To: "Microscopy Microscopy.Com" {Microscopy-at-Microscopy.Com} 10, 25 -- From: Robert Jaeger {rjaeger-at-ikp.tu-darmstadt.de} 10, 25 -- Reply-to: Robert Jaeger {rjaeger-at-ikp.tu-darmstadt.de} 10, 25 -- Subject: Electroscan ESEM2020 10, 25 -- Message-ID: {c9cbdec596eca09a377ed3ce504dc841-at-groupware} 10, 25 -- X-Priority: 3 10, 25 -- X-Mailer: PHPMailer 5.1 (phpmailer.sourceforge.net) 10, 25 -- X-Mailer: FeLaMiMail 10, 25 -- MIME-Version: 1.0 10, 25 -- Content-Type: text/plain; charset="utf-8" 10, 25 -- Content-Transfer-Encoding: 8bit 10, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id x9VFrcLb017240 ==============================End of - Headers==============================
I want to draw your attention to a job posting for a Lab Associate at The George Washington University. This entry level job in a University Microscopy Center 5 blocks from the White House will keep you busy.
Check it out at:
https://www.gwu.jobs/postings/71800
*Dr. Christine A. Brantner* * * The George Washington University* * Senior Research Scientist, Electron Microscopy GW Nanofabrication and Imaging Center 800 22nd Street, NW Science and Engineering Hall B2825 Washington, DC Â 20052
202-994-3219 {tel:202-994-3219} chrisbrantner-at-email.gwu.edu {mailto:chrisbrantner-at-email.gwu.edu} https://nic.gwu.edu */Follow GWNIC on LinkedIN, for updates and exciting news:/**/_https://www.linkedin.com/company/gwnic/_/*
From johnsonloretta061jiypu-at-gmail.com Fri Nov 1 05:44:10 2019 Return-Path: {johnsonloretta061jiypu-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.162.220] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id xA1Ai900023238 for {microscopylistserverarchive7-at-microscopy.com} ; Fri, 1 Nov 2019 05:44:10 -0500 Message-ID: {F74E9AB4.9425EA41-at-gmail.com}
X-from: alice.dohnalkova-at-pnnl.gov
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Email: alice.dohnalkova-at-pnnl.gov Name: Alice Dohnalkova
Organization: EMSL/ PNNL
Title-Subject: [Filtered] Job posting - Assistant Professor of Cryo-Electron Microscopy
Message: Montana State University in Bozeman is currently searching for an Electron Microscopy faculty member. Please distribute this ad in your network as appropriate and have interested parties contact Martin Lawrence at lawrence-at-montana.edu to find out more about the position and search process. 1) The ad is available on the MSU website (https://jobs.montana.edu/postings/18200) 2) Screening of applications will begin November 15, 2019 and will continue until an adequate pool is established.
Bozeman is a beautiful place to live and bring up children and MSU has an amazing faculty, especially in the Biosciences, with immense strengths in thermal biology, biofilms, and virus-/phage-related research.
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Email: rjaeger-at-ikp.tu-darmstadt.de Name: Robert Jaeger
Organization: TU Darmstadt
Title-Subject: [Filtered] Electroscan ESEM2020
Message: Dear all, recently I got an electron beam system which consists of an electron source (gun) with a power supply from Bertan, model no. 2493A.
I looked for more information about such a system on the internet, but unfortunately couldn't find any manual, etc. However, I got some other details, for instance that the system was part of an "Electroscan ESEM2020" (with LaB6 cathode).
Could anyone provide me something like a manual for this ESEM2020 or Bertan power supply? Particulary I'm interested in what the setting "KBF / K / KF" on the front panel could mean. Thanks for any help!
Best regards Robert
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Email: athron-at-ucdavis.edu Name: Andrew Thron
Organization: University of California Davis
Title-Subject: [Filtered] Anomaly in Aperture control on JEOL 2100F
Message: To the microscopy community, My name is Andrew Thron, and I am the Laboratory Manager for the materials characterization lab at UC Davis. We have an anomalous problem with the aperture control board on an aberration correct JEOL 2100F. The first issue appeared with the original aperture control board. A fuse had blown on the board, and when the fuse was replaced and the board was re-installed, several issues arose with the vacuum system. The Penning gauge and the SIP (main vacuum chamber ion pump) shut off. To get the Penning Gauge and the SIP to turn on the entire microscope had to be shut off and restarted. When the microscope restarted the Aperture control board could switch between different apertures (Condenser, Objective, etc.). However, we could not insert/move the aperture drive. We ended up replacing the control board with a used control board from another 2100F.
The used control board worked. However, we had been experiencing anomalous issues with other parts of the microscope. In one instance the microscope did not recognize when the blank holder was being removed, which means the valve to the diffusion pump would not shut when the blank holder was venting. To fix this issue the entire microscope had to be restarted. Another time the value of the accelerating voltage could not be detected by the software, when the gun was actually at 200kV. This issue was fixed by resetting the microscopes hardware/software communication system (VME Board). Finally, we went to insert the smallest condenser lens aperture, and while the aperture was moving the microscope randomly shut off. When the microscope was restarted everything worked fine except for the aperture control board. The board can switch between objective lens and condenser lens apertures. However, the board continues to think the condenser lens aperture #4 is inserted, and we cannot move to another aperture size. When we switch to the objective lens aperture and try to insert an aperture we just hear a beep. There appears to be a problem deeper in the control system. The aperture control board should not affect other subsystems on the microscope (HT, Vacuum, etc.). Has anyone experience an issue like this on a JEOL 2100F or a 2200? The Vacuum control board and the Aperture control board plug into the same power and distribution board. Has anyone experience a short or cross-talk between and aperture control board and the vacuum board on a JEOL 2100F or 2200F?
The local JEOL service engineer has been helping a lot with this issue. I wanted to see if anyone else had experienced something similar or had any insight before we go deep down the rabbit hole. Cheers, Andrew
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From normanatlas54d-at-gmail.com Wed Nov 6 18:20:59 2019 Return-Path: {normanatlas54d-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.162.218] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id xA70KwV0007670 for {microscopylistserverarchive7-at-microscopy.com} ; Wed, 6 Nov 2019 18:20:59 -0600 Received: from smtp4.cyberemailings.com ([151.84.54.233]) by qnx.mdrost.com with ESMTP; Thu, 07 Nov 2019 00:24:16 +0000 Received: from mail.gimmicc.net ([Thu, 07 Nov 2019 00:06:28 +0000]) by rsmail.alkoholic.net with SMTP; Thu, 07 Nov 2019 00:06:28 +0000 Received: from unknown (105.158.90.118) by asx121.turbo-inline.com with SMTP; Thu, 07 Nov 2019 00:03:15 +0000 Received: from qrx.quickslick.com [33.6.227.146] by mx03.listsystemsf.net with SMTP; Wed, 06 Nov 2019 23:55:05 +0000 Received: from unknown (HELO mmx09.tilkbans.com) (Wed, 06 Nov 2019 23:35:33 +0000) by qnx.mdrost.com with NNFMP; Wed, 06 Nov 2019 23:35:33 +0000 Message-ID: {56CE54CB.3F7F31E3-at-gmail.com}
X-from: chair-at-apmc12.in
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Email: chair-at-apmc12.in Name: P Ghosal
Organization: APMC 2020
Title-Subject: [Filtered] Announcement: 12th Asia Pacific Microscopy Conference (APMC-2020)
Message: 12th Asia Pacific Microscopy Conference (APMC-2020)
http://apmc12.in
Important Dates ----------------------- Abstract Submission Deadline: 30th November 2019 Abstract Acceptance Notification: 31st December 2019 Early Bird Registration Deadline: 31st December 2019
About 12th APMC (2020) ------------------------------- Electron Microscope Society of India (EMSI), affiliated to International Federation of Societies for Microscopy (IFSM) is organizing the 12th Asia Pacific Microscopy Conference during 03-07 February, 2020 at Hyderabad, India. The APMC is organized every four years under the auspices of CAPSM–Committee of Asia-Pacific Society for Microscopy. It is anticipated that over a thousand scientists, researchers and students from across the world will be attending. There will be ten parallel sessions along with plenary and keynote lectures by world-renowned speakers to present the advancements in microscopy. A large-scale interactive exhibition where industry will exhibit contemporary technologies will be held.
Call for Papers A forum for sharing the latest ideas and technologies in the world of microscopy and allied techniques especially for the Asia Pacific Microscopists. The conference includes a wide range of topics under three main streams – Material Sciences, Life Sciences and Microscopy Techniques.
Detailed list of various topics for submission of abstracts are listed under CALL FOR ABSTRACTS / TOPICS: https://www.apmc12.in/topics.aspx
Dedicated sessions will include discussions on Microscopy of Metals and Alloys, Glass, Ceramics, Composites, High Energy Materials, Functional Materials, Thin Films and Coatings, Low Dimensional Materials, Electronic and Photonic Materials and other Materials under Material Science.
Microscopy Techniques include Aberration Corrected TEM & STEM, SEM – FIB, Ion Beam Microscopy, Electron Diffraction & Crystallography, EBSD, Orientation Microscopy in TEM/PED, Microscopy based microanalysis, SPM, Spectroscopy analysis, Cryo Electron Microscopy, 3D-APT, In-situ techniques etc.
Sessions under Life Sciences will deal with Microscopy and Imaging associated with Bio Nanotechnology, Medical Applications, Host-Pathogen Interaction, Brain Structure, Animal physiology and Development, Application in Paleontology, Flow Cytometry, Diseases and Immunocyto Chemistry, Multimodal Molecular Imaging in Health & Disease, Imaging methods in medicine, Virology and Taxonomy and other related topics.
Young Researchers, Students and Scholars below the age of 40 are encouraged to submit new and highlighting results in the respective fields which will be evaluated under the category Student / Young Researchers. Registration fee for select Abstracts will be waived off along with an opportunity to present their work in the conference.
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Title-Subject: [Filtered] Job opening Allen Brain specific details
Message: The link I sent yesterday just brings you to the Allen job site. The specific job opening is for a Scientist 1 in Neural Coding department number 2400- We are seeking a Scientist to be an essential member of a team investigating the relationship between the structure of cortical circuits and their function. The project is a large-scale effort combining electron microscopy, two-photon microscopy, genomics and computer modeling to obtain a comprehensive theory of how the mouse visual system codes and processes information.
The Scientist you will lead the effort to develop techniques to prepare and cut large samples of mammalian cortex for electron microscopy. The Scientist should have experience with electron microscopy techniques be able to work in tight collaboration with engineers, microscopists, and physiologists.
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Email: tsmart-at-eastman.com Name: Thomas Smart
Organization: Eastman Chemical Company
Title-Subject: [Filtered] Small size Si wafers
Message: Dear All, Do any of you know of a place that sells 3x3 mm Si wafers? We use them for sandwiching samples to wedge polish to make TEM specimens and currently have to break down large wafers and cut out squares one by one with a low speed diamond wheel saw. If not for purchase perhaps some of you have experience to make this a less tedious process?! Thanks, Thomas
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Most electron microscopy suppliers (Ted Pella, EMS, etc.) will sell you pre-diced Si wafers, though the smallest size they offer is 5x5mm. I found an interesting paper on using a CNC crafting machine (think Cricut and the like) to make 3x3mm diced Si wafers: https://link.springer.com/article/10.1007/s00542-014-2198-4
There are also custom Si wafer suppliers that will pre-dice to any desired spec, though I'm not sure how expensive that would be.
Good luck, Chris
On Thu, Nov 7, 2019 at 8:01 AM {microscopy.listserver-at-gmail.com} wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: tsmart-at-eastman.com } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy to both } tsmart-at-eastman.com, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can } benefit from our collective wisdom } --------------------------------------------------------------------------- } } Email: tsmart-at-eastman.com Name: Thomas Smart } } Organization: Eastman Chemical Company } } Title-Subject: [Filtered] Small size Si wafers } } Message: Dear All, Do any of you know of a place that sells 3x3 mm Si wafers? We use them for } sandwiching samples to wedge polish to make TEM specimens and currently have to break down large } wafers and cut out squares one by one with a low speed diamond wheel saw. If not for purchase } perhaps some of you have experience to make this a less tedious process?! } Thanks, } Thomas } } Login Host: 108.171.130.187 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 9, 53 -- From microscopy.listserver-at-gmail.com Thu Nov 7 06:47:46 2019 } 9, 53 -- Received: from mail-il1-f173.google.com (mail-il1-f173.google.com [209.85.166.173]) } 9, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id xA7Clk72011140 } 9, 53 -- for {microscopy-at-microscopy.com} ; Thu, 7 Nov 2019 06:47:46 -0600 } 9, 53 -- Received: by mail-il1-f173.google.com with SMTP id m5so1715743ilq.0 } 9, 53 -- for {microscopy-at-microscopy.com} ; Thu, 07 Nov 2019 04:56:02 -0800 (PST) } 9, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 9, 53 -- d=gmail.com; s=20161025; } 9, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 9, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 9, 53 -- bh=YN2RQVnkbYIR6MyqJ650qR5GnoHRZMsPmMRDt7yKYho=; } 9, 53 -- b=EcH4ZlDWsHXGuFhxERnaN6hOSDZaeJxTLiRKAmoan6zCI1XxPcFyT+9h682E6GbcFh } 9, 53 -- Ba+OM4MYZHIy3sYF4LMnFgN58ChLf+rLzWwo6Kd78uwdhm/kMYhfneoWYHUWKpubH4oE } 9, 53 -- jLXvezWSx+zT6AlfNB45viw7JRZQmUJP4dyk65R3LKv6uD+c75Biqg922mdeMMRijSPH } 9, 53 -- 1jZnLogGqYGCwQ7g+dg+JQcaQg3lQ47BbcplryvUUSYy1MXihKms5fH2nFaPkUwnvxDu } 9, 53 -- bLpYgb7rodTKIJ/H42HMoJBjxxo7VilaeEhkwMUEXA5f5bvIwtudoFKvKX1nC/mdYP6v } 9, 53 -- PZFA== } 9, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 9, 53 -- d=1e100.net; s=20161025; } 9, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 9, 53 -- :user-agent:mime-version:in-reply-to:content-language } 9, 53 -- :content-transfer-encoding; } 9, 53 -- bh=YN2RQVnkbYIR6MyqJ650qR5GnoHRZMsPmMRDt7yKYho=; } 9, 53 -- b=qN+vg8ML72+RTIY4bZUZkc6pEf6OzD7CbhH0/kScN93jXOXRyfIsRq/7IYE+u3fC81 } 9, 53 -- cAB4II1tWlyU1vlOiPwYYBm7jx5O/zF3BzCyJQCDsQmqCJfgbGMSf5dw6tDzt3fGbTA0 } 9, 53 -- KqI70MzRKU1JEIGYojz7KS0ydE5DpK2cTqx038pjScS6keUhWvwnVs9s5vp4r5GCND8v } 9, 53 -- 2CPA37pFo5mXyIQDiJjhGxdb/EXxbg+3XvhVWmSTQlXo/UWvQhLxscUJ6dTLgkEL4j7k } 9, 53 -- 97wczs+yYfVztqdm6hdEruwjP1sXgnsz9rfJQwIFxqo6cOYFBGl7gkwVc8M0vRKmzAXl } 9, 53 -- SoZg== } 9, 53 -- X-Gm-Message-State: APjAAAWJ3JUpiUVAFtjR+DtyXk5D3cP61PkfLvh1xZE9wEQy3ZM326Qz } 9, 53 -- TkbWoSA0/L4uvJ4PM2y8bffYI3mi } 9, 53 -- X-Google-Smtp-Source: APXvYqwQUMoIUMEemQ47Cr/EkN27qSMHyNAdEkfavUawe3cwIurq49zyiwh6df4FssWWQz6F2ZZ4lw== } 9, 53 -- X-Received: by 2002:a05:6e02:c29:: with SMTP id q9mr4114274ilg.7.1573131362524; } 9, 53 -- Thu, 07 Nov 2019 04:56:02 -0800 (PST) } 9, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:20c5:a55e:af06:459b]) } 9, 53 -- by smtp.googlemail.com with ESMTPSA id k18sm94219ios.31.2019.11.07.04.56.01 } 9, 53 -- for {microscopy-at-microscopy.com} } 9, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 9, 53 -- Thu, 07 Nov 2019 04:56:02 -0800 (PST) } 9, 53 -- Subject: viaWWW:Small size Si wafers } 9, 53 -- References: {201911061411.xA6EBABH011941-at-microscopy.com} } 9, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 9, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 9, 53 -- X-Forwarded-Message-Id: {201911061411.xA6EBABH011941-at-microscopy.com} } 9, 53 -- Message-ID: {3b42c700-cb1e-4538-40fc-eda756dcc4e1-at-gmail.com} } 9, 53 -- Date: Thu, 7 Nov 2019 06:56:01 -0600 } 9, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.14; rv:60.0) } 9, 53 -- Gecko/20100101 Thunderbird/60.9.0 } 9, 53 -- MIME-Version: 1.0 } 9, 53 -- In-Reply-To: {201911061411.xA6EBABH011941-at-microscopy.com} } 9, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 9, 53 -- Content-Language: en-US } 9, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
From tammyhoward072nric-at-gmail.com Thu Nov 7 07:54:40 2019 Return-Path: {tammyhoward072nric-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.162.213] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id xA7DsaPx003446; Thu, 7 Nov 2019 07:54:37 -0600 Received: from smtp.mixedthings.net [180.101.22.85] by mx.reskind.net with SMTP; Thu, 07 Nov 2019 15:47:19 +0200 Message-ID: {6D285914.C468BA2F-at-gmail.com}
Hello All,
Â
Please save the date for another exciting North Atlantic Microscopy Society (NAMS) meeting, April 23rd, 2020 at UPENN, hosted by Andrea Stout, Director of CDB Microscopy Core Facility
Â
8:15 Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Registration
9:00 Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Intro
9:30-10:15 Â Â Â Â Vera Moiseenkova-Bell UPENN (Cryo-EM)
10:15-11:00 Â Â Tim Mosca from Jefferson University (Light)
11:00-11:30 Â Â Break
11:30-12:30 Â Â Tech Bites
12:30-2:00 Â Â Â Â Lunch/Vendors
2:00-3:00 Â Â Â Â Â Â Â Tatyana Svitkina UPENN (CLEM)
3:00-4:00 Â Â Â Â Â Â Â Poster Session/Vendors (snacks/refreshments)
4:00-5:00 Â Â Â Â Â Â Â Justin Taraska NIH (CLEM)
5:00- Social
Pizza Beer
Best,
Gary
==============================Original Headers============================== 19, 37 -- From gary_laevsky-at-yahoo.com Fri Nov 8 11:25:32 2019 19, 37 -- Received: from sonic306-2.consmr.mail.bf2.yahoo.com (sonic306-2.consmr.mail.bf2.yahoo.com [74.6.132.41]) 19, 37 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id xA8HPUgO027182 19, 37 -- for {microscopy-at-microscopy.com} ; Fri, 8 Nov 2019 11:25:30 -0600 19, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1573234431; bh=6hqFkm3NGWjQARI7XYFy6C6UYtodnqpLZZUOoSAoK94=; h=Date:From:To:Subject:References:From:Subject; b=cJZ+ZMIpuowIN8SWfHNoeajdTcZ1tHF3SMltaYXeCvRtCbmMuJOps+TkW1sSS83WzXpQnf+w/hLuCRsE6Acgm65L/+t1lMf3QKh77hiSp2ll9WluRys3czUMpvecRW4OfKl6HWIE/SZuvH5pSphgvDL5LQlV1SLDxZ8QtIywhCLEepDN+2//28oJC147F0vSy3vtgwzap36Tog99Nvousn1aeWADAxG63E670pN3MEfNFxHtcTML3oYpJsyGhIWLxzpsxAonXgxOmjabyKJVOljT4JSITDQ9L30a+yVLFRwxsPVEiz+zu2+dMfZBSWJq1YZS1xV1o2Nw0z2HedxaAg== 19, 37 -- X-YMail-OSG: lhld3iAVM1mkf21GlnKMTcrU8JBWMYwmojB0RmDDHp3ewepXae1YL.BLM7MrHNo 19, 37 -- AlS5AUNzu1JeFXeo7hYMbAvf9s0VzyQCxEeQrdU0YU0LO2d..RTvphAYDjJPaJa5b9JEVruB6WRD 19, 37 -- bMOVuCuNxoNii.F.pgpyW2rsPA8muDZ.5d6hRZdJSPX2o_Q_othRcdHdtY4jfQEXw_kTAUzMC2aE 19, 37 -- E071F4dy0B677jUu3OchU2WkzwdrrtGXiltbtyfJXZxS7p7VWa.iUPh_1y5s0ERIjL43w_XVzqcS 19, 37 -- 2mx8axgzfrNXRWL09lprHPIa7UoEtFQWt7erAcqG95lu9Gp3W6cZQcRqZ.NXAmafpV7egdSN8YX6 19, 37 -- v7l3wSyCgQpT2Q8FQ9Xf4zG7YoqkNhPwn7AjQZ1Y7HD_WrUb4mO_a9GMmgtjhOq_jKlsm.6uVWJ9 19, 37 -- 5LCfug9Aoy6snnJkoZT7uxsAyvAR7mgZ4lZDPZ2DaVK7GUUQJ5X5EWQkJOzoKpCF76bPXMSUw6O6 19, 37 -- 608cNBtBLO76HtLdDP3HJoOsUrzTdAQL31PxAzx7y6onMX4fDZsGEpLENqcww07BEV4Ctos.3kAc 19, 37 -- 9g8Cnlt93bZ4DJFLcSd7rtJG9H2T1YY2b5biOjPjFW6aStekpEzbDN4SKXZMx4s7xeDWrlMJqQ_d 19, 37 -- 8oCQihI8KX.uArsZhtAeE9y5Id4lHECaXyGrrP49NEW1psBQ1859kiQM5wq.CqTa3W8swQPPacD5 19, 37 -- sYYWNvgnQEpRgcrjcSLatSORZtHSc_HmvxOtxPZQ8ynU5iYAPl_zi6Q76_irNesDai3rLrhELeQn 19, 37 -- 7XPzrxAcv99jaPD63ygDhg8yZKlNoe3J57FRhQ2RTxpqRfetqiMFUemC5QIJF77l.s9wEFGKdEpb 19, 37 -- 6475Ji1cc0DsjY.Ie3XKAD1v_ZUY97GYNU1lsVNRP63VWdzpIP4XFuScFrkEaARHTUfY0flBwyeG 19, 37 -- Li8MQASRn_jZJqSEnniDgfD2rmrTMoooq1iC347eiq.4X_ATDn4c_Fer.Qu_Y6Dg134XyPYkpC51 19, 37 -- mJgFpvEaoCiABlHyQLA_8.IrqtIxoyTNIE.0eWT171aSSoPzMY9nx9FVkKok8SVj3wCgt0vwlyLZ 19, 37 -- 7uqaC5lW4hzYCV0sWopTflhMM_uYlWKlSxRCtXWaHxizz__AJNDW6d0pedFj0g6ucOUUch8GPsCE 19, 37 -- CTjwX2eDk3xegjWxDfih5k_tJ70jH0gifbRrrVymrGfgCOtANwaCVmhnQZPiw7t1UncolgEX.cJO 19, 37 -- p6f6hY1Z2kidkUC26NVqnRj68NOXVHLLiSQzE5QO_mJt8QZgObOy6a6RbEle65JD8ofwsgRM9ZP1 19, 37 -- vCaxPee2RKq2to9L7x7_v9kk- 19, 37 -- Received: from sonic.gate.mail.ne1.yahoo.com by sonic306.consmr.mail.bf2.yahoo.com with HTTP; Fri, 8 Nov 2019 17:33:51 +0000 19, 37 -- Date: Fri, 8 Nov 2019 17:33:47 +0000 (UTC) 19, 37 -- From: Gary Laevsky {gary_laevsky-at-yahoo.com} 19, 37 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 19, 37 -- Message-ID: {1831400938.1022296.1573234427044-at-mail.yahoo.com} 19, 37 -- Subject: Save the Date; North Atlantic Microscopy Society (NAMS) meeting, 19, 37 -- April 23rd, 2020 at UPENN 19, 37 -- MIME-Version: 1.0 19, 37 -- Content-Type: text/plain; charset=UTF-8 19, 37 -- References: {1831400938.1022296.1573234427044.ref-at-mail.yahoo.com} 19, 37 -- X-Mailer: WebService/1.1.14680 YMailNorrin Mozilla/5.0 (Macintosh; Intel Mac OS X 10.14; rv:69.0) Gecko/20100101 Firefox/69.0 19, 37 -- Content-Transfer-Encoding: 8bit 19, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id xA8HPUgO027182 ==============================End of - Headers==============================
From efaull-at-hotmail.com Fri Nov 8 22:34:27 2019 Return-Path: {efaull-at-hotmail.com} Received: from mail.iduokan.com ([183.84.4.52]) by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id xA94YQwc000731 for {microscopylistserverarchive7-at-microscopy.com} ; Fri, 8 Nov 2019 22:34:27 -0600 Received: from hotmail.com (unknown [91.110.31.15]) (Authenticated sender: test1-at-iduokan.com) by mail.iduokan.com (Postfix) with ESMTPSA id 0A66612B478 for {microscopylistserverarchive7-at-microscopy.com} ; Sat, 9 Nov 2019 12:34:39 +0800 (CST) Reply-To: draymndch-at-yahoo.co.jp charset="iso-8859-1" Content-Transfer-Encoding: quoted-printable
{HTML} {HEAD} {META name=3DGENERATOR content=3D"MSHTML 11.00.10570.1001"} {/HEAD} {body} I am Vice Chairman of Hang Seng Bank, I have Important Matter to Disc= uss with you concerning my late client. Died without a NEXT OF KIN. Send me= your private email for full details information. email me at {BR} E-Mail: {A= href=3D"mailto:dr29876dr-at-gmail.com"} dr29876dr-at-gmail.com {/A} {BR} Regards {BR= } Mr.Fung {/BODY} {/HTML}
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Dear Listers,
I am looking for simulation code for two-beam diffraction contrast TEM images of dislocations, (preferably free and open source, of course!). I know that this has a long history going back to the 1970s and there have been several implementations (onedis/twodis, comis, cufour, simcon, etc.), some of which have become quite sophisticated. BUT: I can't find any code from a Google search or a trawl through GitHub.
Can anyone point me to a suitable repository or website?
Thanks a lot
Richard Beanland
==============================Original Headers============================== 8, 35 -- From contact-at-integrityscientific.com Mon Nov 11 12:42:19 2019 8, 35 -- Received: from mo4-p00-ob.smtp.rzone.de (mo4-p00-ob.smtp.rzone.de [81.169.146.218]) 8, 35 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id xABIgIlW009947 8, 35 -- for {Microscopy-at-microscopy.com} ; Mon, 11 Nov 2019 12:42:18 -0600 8, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; t=1573498248; 8, 35 -- s=strato-dkim-0002; d=integrityscientific.com; 8, 35 -- h=Date:Message-ID:Subject:From:To:X-RZG-CLASS-ID:X-RZG-AUTH:From: 8, 35 -- Subject:Sender; 8, 35 -- bh=3aUACLXZwHxsKVTQEpkblda1v689isbNXYHRFs27wP8=; 8, 35 -- b=Bfju90xe6Rl4/LZrPlj1sfO4TI/p2iUv2wGu5W1SiHmoeSGm2QprxujykGvKv7GWG6 8, 35 -- NHUswjUaekVr1sWRqK7MyLkHTXz1S0icK2kElKeYNu+alYL6VvsxURx84YIQEm+NoBXq 8, 35 -- 4j6ksnb6N/xOd/6n8KSJn+1VyDJLDlRauNWmc9x3QPcqOKPtjBf2BaY68DcwZFn2PNpK 8, 35 -- skd2s8V2CB/Mhy5KGWW+pWqb+lY+2NU/pHgF4kLak7qm6W/85t6H/GoRGRbAULzuI1LL 8, 35 -- S2bFW/OXYxwigKYR1O6BmjnQOK+BOq+0wILoKzULQknSay4NfLiWlaxy9gw+2YJa2sOA 8, 35 -- 6xSQ== 8, 35 -- X-RZG-AUTH: ":L2MKYUGrb9+u5owo+jDbZQFQdONAbZjDx+2vFy606k0cBSdpvjy8+qJPTfv84pYGUz0DZnaTvApBgPSkTIZvq8b+" 8, 35 -- X-RZG-CLASS-ID: mo01 8, 35 -- Received: from [192.168.1.4] 8, 35 -- by smtp.strato.com (RZmta 44.29.0 DYNA|AUTH) 8, 35 -- with ESMTPSA id j00c2avABIomchl 8, 35 -- (using TLSv1.2 with cipher ECDHE-RSA-AES256-GCM-SHA384 (curve secp521r1 with 521 ECDH bits, eq. 15360 bits RSA)) 8, 35 -- (Client did not present a certificate) 8, 35 -- for {Microscopy-at-microscopy.com} ; 8, 35 -- Mon, 11 Nov 2019 19:50:48 +0100 (CET) 8, 35 -- To: Microscopy-at-microscopy.com 8, 35 -- From: Richard Beanland {contact-at-integrityscientific.com} 8, 35 -- Subject: Cufour, or similar image simulations 8, 35 -- Message-ID: {654dee8c-afb1-621a-1465-07ede3b22094-at-integrityscientific.com} 8, 35 -- Date: Mon, 11 Nov 2019 18:50:49 +0000 8, 35 -- User-Agent: Mozilla/5.0 (Windows NT 10.0; WOW64; rv:68.0) Gecko/20100101 8, 35 -- Thunderbird/68.2.1 8, 35 -- MIME-Version: 1.0 8, 35 -- Content-Type: text/plain; charset=utf-8; format=flowed 8, 35 -- Content-Transfer-Encoding: 8bit 8, 35 -- Content-Language: en-GB ==============================End of - Headers==============================
From fantjame060zclgo-at-gmail.com Tue Nov 12 09:49:59 2019 Return-Path: {fantjame060zclgo-at-gmail.com} Received: from gmail.com (a3.isbfbf.cn [23.228.73.189] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id xACFnwdY002358 for {microscopylistserverarchive7-at-microscopy.com} ; Tue, 12 Nov 2019 09:49:58 -0600 Message-ID: {C4A92852.AFEABD48-at-gmail.com}
X-from: renelle.dubosq-at-gmail.com
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Title-Subject: [Filtered] EGU 2020: Advances in microanalysis: Insights into nanoscale trace element heterogeneities"
Message: Dear colleagues,
We would like to invite submissions of abstracts to our EGU 2020 session applying advanced microanalysis techniques to investigate chemical heterogeneities.
GMPV1.3 "Advances in microanalysis: Insights into nanoscale trace element heterogeneities" Convener: Renelle DubosqECS | Co-conveners: Tyler BlumECS, Sandra Piazolo
Invited speakers: Dr. Desmond Moser, Western University, will present on trace elements and microstructures from Early Mars and the geochronology of habitability
Dr. Ana Cernok, Royal Ontario Museum, will present on shock‐induced microtextures in lunar apatite and merrillite
Please follow this link: https://meetingorganizer.copernicus.org/EGU2020/session/35196 for a full description of the session.
Support application (deadline: 1 December 2019, 13:00 CET)Abstract submission (deadline: 15 January 2020, 13:00 CET)
Looking forward to seeing you in Vienna, Best regards,
Renelle, Tyler and Sandra
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We are evaluating data collection and management practices, and are interested in hearing from other facilities in regards to how data is collected, transferred, and stored. How much data does your facility generate per experiment/session/day? Does your facility have internal infrastructure that enables internal and external users to transfer and store their data? Or does your facility rely on portable storage media and/or external cloud storage options and leave data management up to each user?
For larger data sets, e.g. cryoTEM SPA or tomography acquisitions, x-ray computed tomography, 4DSTEM, etc., do you have special policies in place for storage and transfer? Have you had to pursue upgrading the networking to higher speeds (gigabit, 10 gigabit) to enable working with such data?
Thanks for any feedback you can provide. I'm happy to summarize the responses and provide them to interested parties.
Thanks, Chris
-- Transmission Electron Microscopy Lab Manager Analytical Instrumentation Facility (AIF) NC State University https://www.aif.ncsu.edu/ Cell: 267-496-0587
==============================Original Headers============================== 6, 50 -- From crwinkler-at-ncsu.edu Wed Nov 13 08:02:38 2019 6, 50 -- Received: from mail-il1-f170.google.com (mail-il1-f170.google.com [209.85.166.170]) 6, 50 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id xADE2cQG032236 6, 50 -- for {Microscopy-at-microscopy.com} ; Wed, 13 Nov 2019 08:02:38 -0600 6, 50 -- Received: by mail-il1-f170.google.com with SMTP id m5so1928597ilq.0 6, 50 -- for {Microscopy-at-microscopy.com} ; Wed, 13 Nov 2019 06:11:15 -0800 (PST) 6, 50 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 50 -- d=ncsu.edu; s=google; 6, 50 -- h=mime-version:from:date:message-id:subject:to:cc; 6, 50 -- bh=j88f2y2ecCjy8/baHwiRrwOXiALVUmGH/Mnt7aYJXC8=; 6, 50 -- b=ZClQJbmx31IGFZ0ua7A4o69kxZFH0E3GvGdV3ZRGM6u/Xd8WOZHbSgH3JH+B4qL3IQ 6, 50 -- /wqgPYpDlK9bWu3axEptBZjnneGA94XfmmXo+PQFDd1AFSv2/l7TLWqS09p/NSukGs8q 6, 50 -- dl3cMYGl+oMNcpER+eNSPnlZwCr9XsncOLrE8cKhB8JmSxdNSfGu6Nkjsd0qx43uHCi5 6, 50 -- y7XOT5akeFanR4m4tC/ZC9/iSowNNBIYH81AtNwHtV9jAKgZgJ30wSkfuEf/v24qMXXi 6, 50 -- A6r/0sdFwuaAGnpTPUEQm0r5I2eGlAnz9liUfkV8wYaW0PUf7Udfbr86+2DmFLv+TBM3 6, 50 -- 77lA== 6, 50 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 50 -- d=1e100.net; s=20161025; 6, 50 -- h=x-gm-message-state:mime-version:from:date:message-id:subject:to:cc; 6, 50 -- bh=j88f2y2ecCjy8/baHwiRrwOXiALVUmGH/Mnt7aYJXC8=; 6, 50 -- b=Q6R2q7uozNbsUS7QIArDFFzGnDYGBOnrNyEH2qIjkm5cAk5ks0nFkZzA3/qQftdeTL 6, 50 -- Qa1h5zyBOWokLW9FHEvNZziKI39hz25Kqoh4JJB10Ursn+4z73ssOkVUYHCdxO4wqeev 6, 50 -- tb49g4oWkB+0MC73OZ+I+hGWHqnIafMQr+4pcJGoevXzqwbqmEtE/JdxUSTyhzoZ5Nwp 6, 50 -- SNF1N7p26Wbq0GLDh1KQV69IaGC9itq4KoWyLpXnz2J8PWnnbeDjYWG897ijtYFQUvkU 6, 50 -- bPtvSviGffJd2oJAEI1kN/Tor+N4Gprmrz2kgNEhNJM24Bf6u76cvtyT2dcsQkNCJBcZ 6, 50 -- Q7PA== 6, 50 -- X-Gm-Message-State: APjAAAVRESw7pkZRZXEesS2kRFqQCV3aKfJZGnnN5J1fbXS2eIk/N+rT 6, 50 -- jAcPgvBcRQgIfiJtDS4MhF6ctEb+UYb9cY5FKyu+V4wuRVLPReU7taySgyLw8m4vIijl6lLRLyP 6, 50 -- UykEHml1QT8nJ1NEeDB78ggMkdMMv+KaC6FtdpWGhgr3Y4oLwV2MWzYssoZ+ZRva+2L+u 6, 50 -- X-Google-Smtp-Source: APXvYqzPgo9knYXHlgXd0KN/1Kj5rTgblP8PX6zBxM8xPPTS+1Hpa9ABdKXRAwOekwzpsJ6j48fwIQ== 6, 50 -- X-Received: by 2002:a92:9f5d:: with SMTP id u90mr4353344ili.13.1573654274531; 6, 50 -- Wed, 13 Nov 2019 06:11:14 -0800 (PST) 6, 50 -- Received: from mail-io1-f49.google.com (mail-io1-f49.google.com. [209.85.166.49]) 6, 50 -- by smtp.gmail.com with ESMTPSA id t68sm288298ilb.66.2019.11.13.06.11.14 6, 50 -- for {Microscopy-at-microscopy.com} 6, 50 -- (version=TLS1_3 cipher=TLS_AES_128_GCM_SHA256 bits=128/128); 6, 50 -- Wed, 13 Nov 2019 06:11:14 -0800 (PST) 6, 50 -- Received: by mail-io1-f49.google.com with SMTP id x21so2695154ior.2 6, 50 -- for {Microscopy-at-microscopy.com} ; Wed, 13 Nov 2019 06:11:14 -0800 (PST) 6, 50 -- X-Received: by 2002:a02:c9d5:: with SMTP id c21mr3115864jap.42.1573654273652; 6, 50 -- Wed, 13 Nov 2019 06:11:13 -0800 (PST) 6, 50 -- MIME-Version: 1.0 6, 50 -- From: Christopher Winkler {crwinkler-at-ncsu.edu} 6, 50 -- Date: Wed, 13 Nov 2019 09:11:03 -0500 6, 50 -- X-Gmail-Original-Message-ID: {CAA8T2POvXpCpOWw2bnZyehfd451iM-cS_iMPZk=U-X_mrwT8qQ-at-mail.gmail.com} 6, 50 -- Message-ID: {CAA8T2POvXpCpOWw2bnZyehfd451iM-cS_iMPZk=U-X_mrwT8qQ-at-mail.gmail.com} 6, 50 -- Subject: Data management practices 6, 50 -- To: Microscopy-at-microscopy.com 6, 50 -- Cc: Hsiao-Ying Shadow Huang {hshuang-at-ncsu.edu} 6, 50 -- Content-Type: text/plain; charset="UTF-8" ==============================End of - Headers==============================
The following cryo-ultramicrotome related items are available for only the cost of shipping. Images at https://imgur.com/a/5A6d4Xd
MUST BE CLAIMED BY NOV 22, 2019. Please contact me if interested.
- Cryo attachment for a Leica UC6 ultramicrotome. Saved when our UC6 was traded in for a UC7. Lecia rep suggests PM but otherwise looks good. - related cover - 1 dewar from Leica UC6 UM cryo (would work with UC7). No hose. - 1 much older dewar. Standard connection works with current systems. No hose. - Leica EM CPC parts (incomplete!) - well - some plunger parts - manuals
Best, Wendy
-------------------- Wendy C Salmon, M.A. Light Microscopy Specialist W.M. Keck Facility for Biological Imaging Whitehead Institute for Biomedical Research 455 Main St., Rm 447 Cambridge, MA 02142 e: wsalmon-at-wi.mit.edu c: 617-429-0158 w: microscopy.wi.mit.edu
From michhyma42desqy-at-gmail.com Sat Nov 16 05:24:49 2019 Return-Path: {michhyma42desqy-at-gmail.com} Received: from gmail.com (a3.mg00s0.cn [23.228.73.173] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id xAGBOnci028754 for {microscopylistserverarchive7-at-microscopy.com} ; Sat, 16 Nov 2019 05:24:49 -0600 Message-ID: {B9219A9B.8F61E474-at-gmail.com}
X-from: kate.burgess-at-nrl.navy.mil
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Email: kate.burgess-at-nrl.navy.mil Name: Kate Burgess
Organization: NRL
Title-Subject: [Filtered] Postdoc Position in Electron Microscopy of Planetary Materials
Message: Dear Colleagues,
A position is available now for the study of Solar System and presolar materials at the Naval Research Laboratory in Washington, DC. The successful candidate will have access to state-of-the-art facilities for laboratory study of space weathering of lunar and asteroidal samples, including newly released ANGSA soils, meteoritic organic matter, and preserved stardust in meteorites.
Experience in electron microscopy is required (SEM or TEM). Familiarity with EDS, EELS, and FIB methods are preferred. Experience in one or more complementary analysis methods, such STXM, Raman, IR or atom probe are desirable.
Interested candidates may send inquiries to rhonda.stroud-at-nrl.navy.mil. Please include a CV and publication list. Due to security regulations at NRL, this position requires US citizenship or permanent residency.
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Email: jason_mantei-at-baxter.com Name: Jason Mantei
Organization: Baxter Healthcare
Title-Subject: [Filtered] Midwest M&M Society meeting THIS FRIDAY 11/22 - please join us!
Message: Hello all!
Please join us this Friday for the Fall meeting of the Midwest Microscopy & Microanalysis Society (M3S), a local affiliate society of MSA and MAS! The meeting will take place at Baxter's research park in Round Lake, IL. (For longtime attendees, please note that this is a change from the typical location.)
The meeting, entitled "An Art, and a Science - Topics in Microanalysis" will feature MAS Tour Speaker Ed Vicenzi from the Smithsonian Institute, along with speakers from Argonne National Lab and nearby universities and companies.
The full agenda, abstracts, and driving directions can all be found at our event registration website:
baxter.cvent.com/M3S2019
Please use this website to register for the meeting - it will take just a few minutes and helps us get accurate headcounts.
Any questions, please e-mail me at jason_mantei-at-baxter.com .
Thank you and hope to see you there! Jason
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I am considering using a CeB6 emitter when the current LaB6 emitter in my S/TEM wears out, mostly because of the longer life time. Price difference is minimal. Does any one on the list have experience with CeB6 emitters, and how their performance and lifetime compares to LaB6 in actual use? (I recall varying opinions in the past, but nothing recent.)
------------- Philip Oshel Imaging Facility Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 989 774-3576 office
Our working JEOL 5600LV has to leave the building by mid-December. Unfortunately we don't use it enough to justify the space. We would love to find it a home instead of trashing it, though appreciate that the short time window makes that unlikely.
It is in working order Installed in 12/1999 Continuous service contract coverage. Includes backscatter electron detector and chiller. No EDS.
Only cost is moving costs. JEOL estimates 2 days of service tech effort for breakdown and prep for shipping. Setup would be an additional 2 days.
Please contact me if interested.
Best, Wendy
-------------------- Wendy C Salmon, M.A. Light Microscopy Specialist W.M. Keck Facility for Biological Imaging Whitehead Institute for Biomedical Research 455 Main St., Rm 447 Cambridge, MA 02142 e: wsalmon-at-wi.mit.edu c: 617-429-0158 w: microscopy.wi.mit.edu
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Email: jtwilley-at-sprynet.com Name: John Twilley
Organization: Stony Brook University
Title-Subject: [Filtered] GaN on sapphire trims, scraps
Message: Looking for small wafer or fragment of gallium nitride deposited on sapphire (or silicon) for X-ray materials study. Doping, if any, doesn't matter because electrical properties are unimportant. Dimensions could be as small as 2 x 2mm. Does anyone have any scrap?
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We have an old (1993 vintage) JEOL 2010 which until recently has been running well.
Yesterday when I arrived at the laboratory it had shut itself down. Upon restart everything seemed OK, but it shut itself down again. I tried another time and the same thing happened.
Today I have done the same thing, but I had the opportunity to monitor the process. It has gone thought its normal start up procedure and got to the normal state, with all the pressures at levels that would be expected. About 10 minutes later it shut itself down with no warning. No alarms or alerts sounded it just shut down. I have checked the water supply, power supply, RP oil etc and everything seems OK.
I was wondering if anyone else had experience this type of issue before and if they had a solution.
E colin.veitch-at-csiro.au T +61 3 5246 4891 M 0438 538 475 F +61 3 5246 4057 Address CSIRO Manufacturing, 75 Pigdons Road, Waurn Ponds, Vic 3216, Australia. www.csiro.au | http://www.csiro.au/Organisation-Structure/Flagships/Manufacturing.aspx
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looks like something coming either from the cooling flow rate or the power-supplies.
Check:
- do you have a sufficient flow rate at the "hot" tap coming from the scope (best would be to take off the hose and check) ?
- once I had this problem at older EM4x0 TEMs. It came from some varistors used as a current surge protection across the output pins in the instrument`s power supplies. The varistors got old. After exchange everything had been fine. For function see https://en.wikipedia.org/wiki/Varistor
Best wishes,
Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Am 20.11.19 um 03:39 schrieb Colin.Veitch-at-csiro.au: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi, } } We have an old (1993 vintage) JEOL 2010 which until recently has been running well. } } Yesterday when I arrived at the laboratory it had shut itself down. Upon restart everything seemed OK, but it shut itself down again. I tried another time and the same thing happened. } } Today I have done the same thing, but I had the opportunity to monitor the process. It has gone thought its normal start up procedure and got to the normal state, with all the pressures at levels that would be expected. About 10 minutes later it shut itself down with no warning. No alarms or alerts sounded it just shut down. I have checked the water supply, power supply, RP oil etc and everything seems OK. } } I was wondering if anyone else had experience this type of issue before and if they had a solution. } } Thank you in anticipation. } } Kind regards, } } Colin Veitch } } Manager, Microscopy - Waurn Ponds Laboratory } CSIRO Manufacturing } } E colin.veitch-at-csiro.au T +61 3 5246 4891 M 0438 538 475 F +61 3 5246 4057 } Address CSIRO Manufacturing, 75 Pigdons Road, Waurn Ponds, Vic 3216, Australia. } www.csiro.au | http://www.csiro.au/Organisation-Structure/Flagships/Manufacturing.aspx } } CSIRO acknowledges the Traditional Owners of the lands that we live and work on across Australia and pays its respect to Elders past, present and emerging. } } } } PLEASE NOTE } The information contained in this email may be confidential or privileged. Any unauthorised use or disclosure is prohibited. If you have received this email in error, please delete it immediately and notify the sender by return email. Thank you. 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==============================Original Headers============================== 11, 29 -- From diller-at-stefan-diller.com Wed Nov 20 03:25:56 2019 11, 29 -- Received: from mailout031.rox.net (mailout031.rox.net [212.63.85.231]) 11, 29 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id xAK9Ptux020240 11, 29 -- for {microscopy-at-microscopy.com} ; Wed, 20 Nov 2019 03:25:55 -0600 11, 29 -- Received: from localhost ([127.0.0.1]) 11, 29 -- by mailout03.rox.net with esmtp (Exim 4.80) 11, 29 -- (envelope-from {diller-at-stefan-diller.com} ) 11, 29 -- id 1iXMNx-0003L3-8G; Wed, 20 Nov 2019 10:34:53 +0100 11, 29 -- Received: from ip1f10fad8.dynamic.kabel-deutschland.de ([31.16.250.216] helo=mac-pro.local) 11, 29 -- by mailout03.rox.net with esmtpsa (TLSv1.2:DHE-RSA-AES128-SHA:128) 11, 29 -- (Exim 4.80) 11, 29 -- (envelope-from {diller-at-stefan-diller.com} ) 11, 29 -- id 1iXMNw-0003Km-Sx; Wed, 20 Nov 2019 10:34:52 +0100 11, 29 -- Subject: Re: [Microscopy] JEOL 2010 problem 11, 29 -- To: Colin.Veitch-at-csiro.au, 11, 29 -- "Microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 11, 29 -- References: {201911200239.xAK2dUUm006702-at-microscopy.com} 11, 29 -- From: stefan diller {diller-at-stefan-diller.com} 11, 29 -- Message-ID: {4479dea0-533a-8e65-418a-5b956ab86b09-at-stefan-diller.com} 11, 29 -- Date: Wed, 20 Nov 2019 10:34:51 +0100 11, 29 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:68.0) 11, 29 -- Gecko/20100101 Thunderbird/68.2.1 11, 29 -- MIME-Version: 1.0 11, 29 -- In-Reply-To: {201911200239.xAK2dUUm006702-at-microscopy.com} 11, 29 -- Content-Type: text/plain; charset=windows-1252; format=flowed 11, 29 -- Content-Transfer-Encoding: 7bit 11, 29 -- Content-Language: en-GB 11, 29 -- X-Envelope-From: {diller-at-stefan-diller.com} 11, 29 -- X-Scanned-By: rockenstein AG ==============================End of - Headers==============================
From trojlita384hipj-at-gmail.com Wed Nov 20 06:45:28 2019 Return-Path: {trojlita384hipj-at-gmail.com} Received: from gmail.com (a6.paq819.cn [23.228.73.180] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id xAKCjR3h001815 for {microscopylistserverarchive7-at-microscopy.com} ; Wed, 20 Nov 2019 06:45:27 -0600 Message-ID: {890C3B45.1657659E-at-gmail.com}
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Email: ravi.thakkar369-at-gmail.com Name: Ravi Title-Subject: [Filtered] Staining of Cys containing peptide with Methyl Mercury Chloride.
Message: Hi, Does anyone have a protocol to stain peptide with Methyl Mercury chloride to tag Cys residue?
What safety precautions are needed to work on this chemical?
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X-from: Pappas, Richard Steve (CDC/DDNID/NCEH/DLS) {rlp6-at-cdc.gov}
Mercury(II) chloride will also tag cysteine and methionine sulfur and is safer than organomercury. It soaks through skin at a slower rate. Why do you want to use organomercury?
R. Steven Pappas, Ph.D. Team Lead, Tobacco Inorganics Group M.S. S110-4 Centers for Disease Control & Prevention 4770 Buford Highway, NE Atlanta, GA 30341-3717, USA
-----Original Message----- X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Wednesday, November 20, 2019 8:14 AM To: Pappas, Richard Steve (CDC/DDNID/NCEH/DLS) {rlp6-at-cdc.gov}
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Email: ravi.thakkar369-at-gmail.com Name: Ravi Title-Subject: [Filtered] Staining of Cys containing peptide with Methyl Mercury Chloride.
Message: Hi, Does anyone have a protocol to stain peptide with Methyl Mercury chloride to tag Cys residue?
What safety precautions are needed to work on this chemical?
I have an old MT 5000 ultramicrotome I am trying to resurrect. Oddly, it seems to have flipped the upstroke with the downstroke. So the light indicators on the arm position show it is going down on the return stroke and applies the differential speed setting and length of stroke accordingly to the upstroke. Has anyone seen this occur before? It has both me and the owner of the instrument perplexed. I assume 39mm/sec would be quite fast to section with as this makes then cutting stroke the full speed.
Any ideas would be appreciated. Thank you.
Regards -Jason
==============================Original Headers============================== 4, 48 -- From rockman507-at-gmail.com Thu Nov 21 06:08:55 2019 4, 48 -- Received: from mail-qv1-f53.google.com (mail-qv1-f53.google.com [209.85.219.53]) 4, 48 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id xALC8t90025252 4, 48 -- for {Microscopy-at-microscopy.com} ; Thu, 21 Nov 2019 06:08:55 -0600 4, 48 -- Received: by mail-qv1-f53.google.com with SMTP id s18so1292615qvr.4 4, 48 -- for {Microscopy-at-microscopy.com} ; Thu, 21 Nov 2019 04:17:58 -0800 (PST) 4, 48 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 48 -- d=gmail.com; s=20161025; 4, 48 -- h=content-transfer-encoding:from:mime-version:subject:message-id:date 4, 48 -- :to; 4, 48 -- bh=pCfBhk55EogJ4SQ2cNwJ2oxNmhNhrGsdtMU93dMpV7I=; 4, 48 -- b=f1saTYr0uN78ChvpqcaNn1R6Px7E5UD+Uu4uwvOqR9p8+JP0yJv8QLf9fuBsOKGko8 4, 48 -- tjsVqhJ4kqzD0imeEiBQbgkwgo+vH1ATQH5v/OaKV28pf2nqcHYU2Wndm9+U5np/9Bo1 4, 48 -- 0qD6tGCYpOxeAGMPVJSbxvzmn1Aqa9aSwbOhPcavLw9qQ1ljiRL6uR1XF46/kKdl4bQf 4, 48 -- hmMDEKM3RODFzMnYPuYcNV72tVHeNUYsEurNbpkj8YrZsjfNiDmY6vtOkYQpcAvjqzSj 4, 48 -- d349SbZZykis6D2w22/elOgWJ8wFNFZ8fZ5cXy+KEtMEAYnSF/HxAxYCwTT6AuOrnnA4 4, 48 -- 7dYQ== 4, 48 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 48 -- d=1e100.net; s=20161025; 4, 48 -- h=x-gm-message-state:content-transfer-encoding:from:mime-version 4, 48 -- :subject:message-id:date:to; 4, 48 -- bh=pCfBhk55EogJ4SQ2cNwJ2oxNmhNhrGsdtMU93dMpV7I=; 4, 48 -- b=pDTXOOwSd1h1Z4zFTtaGOUXMKua6m92GBHRKkPdtNsjonWKZKU2UFObVffbZWQvDdC 4, 48 -- OI1VUng4fMCtTbRWANsS3GOd2AFKOHBeOZVnYPlBt02MF7Lqk5nSYOlHM7U0rTbJvVYb 4, 48 -- vm1/+W7CdiD8K94rDcnd/j0K8Bw7zRwZNvSFHdZ553RzXx/jOa1S21Z8dG2uTg3ftAPW 4, 48 -- HrBnnoH3mnCj/VGrf+uNTDncAGE0ehnu0vAQyinhLS3g5UX/XjYCXh96drc+latiE2M7 4, 48 -- /wMRW7YO0mmSE7T6ihqiOedBb+VAxQHBfe0enwM+Jm3xKnD6kSsItQ6TWkxKH9LajWkh 4, 48 -- l6Lw== 4, 48 -- X-Gm-Message-State: APjAAAXESsVDaQzmz65SC2DPVsRBX4aefUZXU64rrAhsknfRNjujphSb 4, 48 -- zv5kA6Ho+eDyFc2shAaesw/sYrzZ 4, 48 -- X-Google-Smtp-Source: APXvYqxwPWBR41rcLpPt1wkRd8beQSSc6bFZqLe4xs4tuGfHm2K2wi/Mnqdqpy0KdVkAa5bt36KVgg== 4, 48 -- X-Received: by 2002:a05:6214:170c:: with SMTP id db12mr7906703qvb.202.1574338677724; 4, 48 -- Thu, 21 Nov 2019 04:17:57 -0800 (PST) 4, 48 -- Received: from [10.108.89.173] (wirelessNAT189.wireless.temple.edu. [155.247.134.189]) 4, 48 -- by smtp.gmail.com with ESMTPSA id j18sm1342612qtn.52.2019.11.21.04.17.56 4, 48 -- for {Microscopy-at-microscopy.com} 4, 48 -- (version=TLS1_3 cipher=TLS_AES_128_GCM_SHA256 bits=128/128); 4, 48 -- Thu, 21 Nov 2019 04:17:56 -0800 (PST) 4, 48 -- Content-Type: text/plain; charset=us-ascii 4, 48 -- From: Jason Saredy {rockman507-at-gmail.com} 4, 48 -- Mime-Version: 1.0 (1.0) 4, 48 -- Subject: Sorvall MT 5000 troubleshooting 4, 48 -- Message-Id: {FF0B8DA7-B203-4D80-B222-28A1926A1FB5-at-gmail.com} 4, 48 -- Date: Thu, 21 Nov 2019 07:17:56 -0500 4, 48 -- To: Microscopy-at-microscopy.com 4, 48 -- X-Mailer: iPhone Mail (17A878) 4, 48 -- Content-Transfer-Encoding: 8bit 4, 48 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id xALC8t90025252 ==============================End of - Headers==============================
I am did a lot of repairs of the older Sorvall / RMC MT-5000s.
Since I do not know what kind of repair you did, this effect might come from:
- mid of stroke not aligned (you need to unfasten the old decoder from the latter chain, set the specimen position to 12mm above knife trough and fasten the decoder with the ladder belt in such a way that the LED on the Visutrac are in mid)
- some IR led or photo-transistor in the decoder pcb not working
- you changed the motor but you also interchanged the pins of the tacho-generator; this results in unstopping, very fast drive of the arm movement
At which point of the stroke do you see velocity change of the arm? Can you change the retract speed?
Best wishes,
Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Am 21.11.19 um 13:16 schrieb rockman507-at-gmail.com: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello all } } I have an old MT 5000 ultramicrotome I am trying to resurrect. Oddly, it seems to have flipped the upstroke with the downstroke. So the light indicators on the arm position show it is going down on the return stroke and applies the differential speed setting and length of stroke accordingly to the upstroke. Has anyone seen this occur before? It has both me and the owner of the instrument perplexed. I assume 39mm/sec would be quite fast to section with as this makes then cutting stroke the full speed. } } Any ideas would be appreciated. Thank you. } } Regards } -Jason } } ==============================Original Headers============================== } 4, 48 -- From rockman507-at-gmail.com Thu Nov 21 06:08:55 2019 } 4, 48 -- Received: from mail-qv1-f53.google.com (mail-qv1-f53.google.com [209.85.219.53]) } 4, 48 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id xALC8t90025252 } 4, 48 -- for {Microscopy-at-microscopy.com} ; Thu, 21 Nov 2019 06:08:55 -0600 } 4, 48 -- Received: by mail-qv1-f53.google.com with SMTP id s18so1292615qvr.4 } 4, 48 -- for {Microscopy-at-microscopy.com} ; Thu, 21 Nov 2019 04:17:58 -0800 (PST) } 4, 48 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 4, 48 -- d=gmail.com; s=20161025; } 4, 48 -- h=content-transfer-encoding:from:mime-version:subject:message-id:date } 4, 48 -- :to; } 4, 48 -- bh=pCfBhk55EogJ4SQ2cNwJ2oxNmhNhrGsdtMU93dMpV7I=; } 4, 48 -- b=f1saTYr0uN78ChvpqcaNn1R6Px7E5UD+Uu4uwvOqR9p8+JP0yJv8QLf9fuBsOKGko8 } 4, 48 -- tjsVqhJ4kqzD0imeEiBQbgkwgo+vH1ATQH5v/OaKV28pf2nqcHYU2Wndm9+U5np/9Bo1 } 4, 48 -- 0qD6tGCYpOxeAGMPVJSbxvzmn1Aqa9aSwbOhPcavLw9qQ1ljiRL6uR1XF46/kKdl4bQf } 4, 48 -- hmMDEKM3RODFzMnYPuYcNV72tVHeNUYsEurNbpkj8YrZsjfNiDmY6vtOkYQpcAvjqzSj } 4, 48 -- d349SbZZykis6D2w22/elOgWJ8wFNFZ8fZ5cXy+KEtMEAYnSF/HxAxYCwTT6AuOrnnA4 } 4, 48 -- 7dYQ== } 4, 48 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 4, 48 -- d=1e100.net; s=20161025; } 4, 48 -- h=x-gm-message-state:content-transfer-encoding:from:mime-version } 4, 48 -- :subject:message-id:date:to; } 4, 48 -- bh=pCfBhk55EogJ4SQ2cNwJ2oxNmhNhrGsdtMU93dMpV7I=; } 4, 48 -- b=pDTXOOwSd1h1Z4zFTtaGOUXMKua6m92GBHRKkPdtNsjonWKZKU2UFObVffbZWQvDdC } 4, 48 -- OI1VUng4fMCtTbRWANsS3GOd2AFKOHBeOZVnYPlBt02MF7Lqk5nSYOlHM7U0rTbJvVYb } 4, 48 -- vm1/+W7CdiD8K94rDcnd/j0K8Bw7zRwZNvSFHdZ553RzXx/jOa1S21Z8dG2uTg3ftAPW } 4, 48 -- HrBnnoH3mnCj/VGrf+uNTDncAGE0ehnu0vAQyinhLS3g5UX/XjYCXh96drc+latiE2M7 } 4, 48 -- /wMRW7YO0mmSE7T6ihqiOedBb+VAxQHBfe0enwM+Jm3xKnD6kSsItQ6TWkxKH9LajWkh } 4, 48 -- l6Lw== } 4, 48 -- X-Gm-Message-State: APjAAAXESsVDaQzmz65SC2DPVsRBX4aefUZXU64rrAhsknfRNjujphSb } 4, 48 -- zv5kA6Ho+eDyFc2shAaesw/sYrzZ } 4, 48 -- X-Google-Smtp-Source: APXvYqxwPWBR41rcLpPt1wkRd8beQSSc6bFZqLe4xs4tuGfHm2K2wi/Mnqdqpy0KdVkAa5bt36KVgg== } 4, 48 -- X-Received: by 2002:a05:6214:170c:: with SMTP id db12mr7906703qvb.202.1574338677724; } 4, 48 -- Thu, 21 Nov 2019 04:17:57 -0800 (PST) } 4, 48 -- Received: from [10.108.89.173] (wirelessNAT189.wireless.temple.edu. [155.247.134.189]) } 4, 48 -- by smtp.gmail.com with ESMTPSA id j18sm1342612qtn.52.2019.11.21.04.17.56 } 4, 48 -- for {Microscopy-at-microscopy.com} } 4, 48 -- (version=TLS1_3 cipher=TLS_AES_128_GCM_SHA256 bits=128/128); } 4, 48 -- Thu, 21 Nov 2019 04:17:56 -0800 (PST) } 4, 48 -- Content-Type: text/plain; charset=us-ascii } 4, 48 -- From: Jason Saredy {rockman507-at-gmail.com} } 4, 48 -- Mime-Version: 1.0 (1.0) } 4, 48 -- Subject: Sorvall MT 5000 troubleshooting } 4, 48 -- Message-Id: {FF0B8DA7-B203-4D80-B222-28A1926A1FB5-at-gmail.com} } 4, 48 -- Date: Thu, 21 Nov 2019 07:17:56 -0500 } 4, 48 -- To: Microscopy-at-microscopy.com } 4, 48 -- X-Mailer: iPhone Mail (17A878) } 4, 48 -- Content-Transfer-Encoding: 8bit } 4, 48 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id xALC8t90025252 } ==============================End of - Headers==============================
==============================Original Headers============================== 14, 29 -- From diller-at-stefan-diller.com Thu Nov 21 06:42:00 2019 14, 29 -- Received: from mailout031.rox.net (mailout031.rox.net [212.63.85.231]) 14, 29 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id xALCg002001024 14, 29 -- for {microscopy-at-microscopy.com} ; Thu, 21 Nov 2019 06:42:00 -0600 14, 29 -- Received: from localhost ([127.0.0.1]) 14, 29 -- by mailout03.rox.net with esmtp (Exim 4.80) 14, 29 -- (envelope-from {diller-at-stefan-diller.com} ) 14, 29 -- id 1iXlvK-0002tt-2q; Thu, 21 Nov 2019 13:51:02 +0100 14, 29 -- Received: from ip1f10fad8.dynamic.kabel-deutschland.de ([31.16.250.216] helo=mac-pro.local) 14, 29 -- by mailout03.rox.net with esmtpsa (TLSv1.2:DHE-RSA-AES128-SHA:128) 14, 29 -- (Exim 4.80) 14, 29 -- (envelope-from {diller-at-stefan-diller.com} ) 14, 29 -- id 1iXlvJ-0002tQ-Qx; Thu, 21 Nov 2019 13:51:01 +0100 14, 29 -- Subject: Re: [Microscopy] Sorvall MT 5000 troubleshooting 14, 29 -- To: rockman507-at-gmail.com, 14, 29 -- "Microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 14, 29 -- References: {201911211216.xALCGSPB032152-at-microscopy.com} 14, 29 -- From: stefan diller {diller-at-stefan-diller.com} 14, 29 -- Message-ID: {8c785a5d-0a4a-e44c-7f4d-6e47a55c16c3-at-stefan-diller.com} 14, 29 -- Date: Thu, 21 Nov 2019 13:51:01 +0100 14, 29 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:68.0) 14, 29 -- Gecko/20100101 Thunderbird/68.2.1 14, 29 -- MIME-Version: 1.0 14, 29 -- In-Reply-To: {201911211216.xALCGSPB032152-at-microscopy.com} 14, 29 -- Content-Type: text/plain; charset=windows-1252; format=flowed 14, 29 -- Content-Transfer-Encoding: 7bit 14, 29 -- Content-Language: en-GB 14, 29 -- X-Envelope-From: {diller-at-stefan-diller.com} 14, 29 -- X-Scanned-By: rockenstein AG ==============================End of - Headers==============================
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Email: Hui_Yang1-at-uml.edu Name: Hui Yang
Organization: University of Massachusetts Lowell
Title-Subject: [Filtered] Retain Chlorophyll in Sections
Message: Hello everyone, I am studying an animal-algae symbiosis. The algae invades animal tissue and has chlorophyll so I can usually see them in fixed animals using a fluorescence microscope. However, now I need to make some thin sections to get a closer look and most dehydration and infiltration procedures would wash away the chlorophyll. Does anyone know any embedding material that may be able to retain the chlorophyll? My specimens are 5-8 mm in length but I can cut out small pieces of 1-2 mm. The goal is to distinguish the algae from animal cells. I am hoping to cut 5-20 um sections, either paraffin or resin is fine. Thanks Hui
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Hi, Dear Hui, would you mind to inform a bit more in detail what you are going to do with your samples (any more specific info available?). For me it isn't clear whether you only will examine "thin sections" (= i.e. IMHO semithin resin sections) or if you will process specimens via the routine processing route for either SEM or TEM (i.e. "ultrathin" sections for the latter?)
I am sure there might be (older/ancient) protocols to prevent particular elusion or even complete loss of chlorophyll within the symbiontic algae...
5 - 20 (!)µm sections (excuse me) might be a bit thick for LM-or EM-examination.....but I don't know really what you will be doing (which method/technique....)....
Thanking in advance for clarification...
Wolfgang
PS: added just only for convenience (no warranty for mentioning a precise technology) Azaman et al, 2017 ( DOI 10.7717/peerj.3473 ) A comparison of the morphological and biochemical characteristics of Chlorella sorokiniana and Chlorella zofingiensis cultured under photoautotrophic and mixotrophic conditions (OPEN ACCESS PDF cf.: https://peerj.com/articles/3473.pdf)
Eventually [LM, TEM, SEM] also for implementing control specimens , e.g. by by proactive removal of Chlorophyll), cf.: Li et al, 2016: A Saponification Method for Chlorophyll Removal from Microalgae Biomass as Oil Feedstock Mar Drugs. 2016 Sep; 14(9): 162. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5039533/ PDF: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5039533/pdf/marinedrugs-14-0016 2.pdf
SCHMITT et al, 2014: Chloroplast incorporation and long-term photosynthetic performance through the life cycle in laboratory cultures of Elysia timida (Sacoglossa, Heterobranchia) Front Zool. 2014; 11: 5. Published online 2014 Jan 16. doi: 10.1186/1742-9994-11-5, cf.: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3898781/ or PDF: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3898781/pdf/1742-9994-11-5.pdf
================================================================= MUSS Wolfgang Dr. phil. [OR i. R.] Ignaz-Rieder-Kai 19/6 A-5020 SALZBURG Österreich-AUSTRIA
Honestly added: FRMS, Retired Member of MSA & other (Inter-)National Societies Former Head of Electron Microscopy Lab at Institute of Pathology SALK-LKH / Salzburger Landeskliniken | General Hospital and PMU (private) PARACELSUS MEDICAL UNIVERSITY of SALZBURG Scientific Profile at ResearchGate: http://www.researchgate.net/profile/Wolfgang_MUSS inviting you to join RG (Sign up - ResearchGate -at- https://www.researchgate.net/signup.SignUp.html, and join 14+ million researchers, including 63 Nobel Laureates)
Former Secretary Long standing Member (until March 2018) and (until June2017) Board Member of the
SCUR {The Society for Cutaneous Ultrastructure Research} „The Skin Imaging Society“ { www.scur.org }
============================================================================ =========== Von: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Gesendet: Donnerstag, 21. November 2019 13:52 An: wij.muss-at-aon.at Betreff: [Microscopy] Retain Chlorophyll in Sections....
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please copy to both Hui_Yang1-at-uml.edu, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom --------------------------------------------------------------------------- Email: Hui_Yang1-at-uml.edu Name: Hui Yang Organization: University of Massachusetts Lowell Title-Subject: Retain Chlorophyll in Sections Message:
Hello everyone, I am studying an animal-algae symbiosis. The algae invades animal tissue and has chlorophyll so I can usually see them in fixed animals using a fluorescence microscope. However, now I need to make some thin sections to get a closer look and most dehydration and infiltration procedures would wash away the chlorophyll.
Does anyone know any embedding material that may be able to retain the chlorophyll? My specimens are 5-8 mm in length but I can cut out small pieces of 1-2 mm. The goal is to distinguish the algae from animal cells. I am hoping to cut 5-20 um sections, either paraffin or resin is fine.
Thanks Hui
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I'm advertising a 3-year postdoc position in atomic-scale structure determination in thick nanostructures at the School of Physics and Astronomy, Monash University, Australia. Further details below.
Please bring this to the attention of anyone who may be interested.
Many thanks, Scott Findlay
___________________
Position Descriptions and application details at: http://careers.pageuppeople.com/513/cw/en/job/600527/research-fellow-in-atomicscale-structure-determination-in-thick-nanostructures
Position overview: The Research Fellow will work on developing methods for atomic-scale structure determination via scanning transmission electron microscopy. This project aims to develop a theoretical and computational toolkit for structure retrieval at atomic resolution that is robust in the presence of multiple scattering (“dynamical diffraction”) of the electron probe, and to apply it to large experimental data sets obtained from the new generation of fast-readout electron detectors. The project may draw on methods from inverse scattering theory, phase retrieval, iterative algorithms, machine learning, and high-performance computing.
==============================Original Headers============================== 8, 51 -- From scott.findlay-at-monash.edu Thu Nov 21 14:59:18 2019 8, 51 -- Received: from mail-pl1-f172.google.com (mail-pl1-f172.google.com [209.85.214.172]) 8, 51 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id xALKxIZm008122 8, 51 -- for {Microscopy-at-microscopy.com} ; Thu, 21 Nov 2019 14:59:18 -0600 8, 51 -- Received: by mail-pl1-f172.google.com with SMTP id bb5so2152354plb.4 8, 51 -- for {Microscopy-at-microscopy.com} ; Thu, 21 Nov 2019 13:08:22 -0800 (PST) 8, 51 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 8, 51 -- d=monash.edu; s=google; 8, 51 -- h=from:subject:to:message-id:date:user-agent:mime-version 8, 51 -- :content-language:content-transfer-encoding; 8, 51 -- bh=k+Vb8UdrN/T19U28PTje8y7boU+StxsyI4UMlhlbGUo=; 8, 51 -- b=at2enHo+rWD97Dpr14wWdZKnWYAV2/Tt+ry3IuLMWcHllZ6Q7uvl5anOl16nf/paJz 8, 51 -- eXxPIFV09/mxXx2XamLI8DtmTP7qh1rEIKHBcbgaPKh4Pbedtw8/0+DuRQDsJDRKlix/ 8, 51 -- cjWMcNl+eJFoLCwBhzXC6wGSnrEqY7i3/Jo1DJtor4hyvSuWKZsuJzldIS4AYhKozXnc 8, 51 -- Dmck+ipAukBBya4Zn1ohvq3Rv3ivnjZL5nE25n3QIihxf10Pd14asEKaRIyOtO40ARB6 8, 51 -- TOqnMZMXTtbD6aP/VauHeGcu4l+E3eLFNoZaZi56zdnfzWIy21t6e3jsIzIP/a6Ukl3o 8, 51 -- TiBg== 8, 51 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 8, 51 -- d=1e100.net; s=20161025; 8, 51 -- h=x-gm-message-state:from:subject:to:message-id:date:user-agent 8, 51 -- :mime-version:content-language:content-transfer-encoding; 8, 51 -- bh=k+Vb8UdrN/T19U28PTje8y7boU+StxsyI4UMlhlbGUo=; 8, 51 -- b=oXTFmnQmhXzY28rcnHq3xUw6qjfkny7bNUeI6PDyaEB5yvm1EqkCAOeX5BMEj0lGZP 8, 51 -- WvUIcgVnaQN5geukOnTKcb9NlFze5pTBw4sq/lk4WHBSD+BTH8FMls+PSgD1SEkq2EoS 8, 51 -- Jpk4uqJL4SVjCQnf2YevEZB2vUNKhs7goQgXBLeH4H+XyJdRGVSXLTQlh1/+scFn8Eg/ 8, 51 -- eIFMjx0e7oW7Oda3Fc6T2Zn1DX5yQEIy9wQA1XusptXIezoxp0lSDgf6Tqzj9Fq1Q05d 8, 51 -- SdL8angGC0DaZXA8RmaSrfleE38t95CRUjB0oDxZv8iAigRHBGNsQLyaPe/pYFcFjpc0 8, 51 -- QofQ== 8, 51 -- X-Gm-Message-State: APjAAAXWdHELmt4w7GEwfLgPS4bgxL4NXQGOT6nswh85kcxahyvBOWdt 8, 51 -- 3idKCQzNf792ez7xxgd5OrVGj12pKMYC46C25aAjvymK4u6vutIKjdLcNXypxyAe2wnVbPdaxrY 8, 51 -- kFlAjCSihhNheABsKX+HX2aI1PUf7+KMOzBDg2wmHQ9lb5rk8YXRwtvBc9soghWGgP8EfJyAhz/ 8, 51 -- /J 8, 51 -- X-Google-Smtp-Source: APXvYqxwewr+Eu2tBs/YcHXl9Aja25Uij8EROcAk7bK8CWh3HWWI6OQHfWP0os/0aCGd1wDXAU7HcQ== 8, 51 -- X-Received: by 2002:a17:90a:ead5:: with SMTP id ev21mr14143883pjb.76.1574370500914; 8, 51 -- Thu, 21 Nov 2019 13:08:20 -0800 (PST) 8, 51 -- Received: from ?IPv6:2001:388:608c:4c73:fcce:eb9a:6a95:7f2c? ([2001:388:608c:4c73:fcce:eb9a:6a95:7f2c]) 8, 51 -- by smtp.gmail.com with ESMTPSA id 4sm4484978pfz.185.2019.11.21.13.08.19 8, 51 -- for {Microscopy-at-microscopy.com} 8, 51 -- (version=TLS1_3 cipher=TLS_AES_128_GCM_SHA256 bits=128/128); 8, 51 -- Thu, 21 Nov 2019 13:08:20 -0800 (PST) 8, 51 -- From: Scott Findlay {scott.findlay-at-monash.edu} 8, 51 -- Subject: Postdoc position in STEM at Monash University 8, 51 -- To: Microscopy-at-microscopy.com 8, 51 -- Message-ID: {94a273ed-2ee7-b999-d34f-b43d718753f8-at-monash.edu} 8, 51 -- Date: Fri, 22 Nov 2019 08:08:17 +1100 8, 51 -- User-Agent: Mozilla/5.0 (Windows NT 10.0; WOW64; rv:68.0) Gecko/20100101 8, 51 -- Thunderbird/68.2.2 8, 51 -- MIME-Version: 1.0 8, 51 -- Content-Type: text/plain; charset=utf-8; format=flowed 8, 51 -- Content-Language: en-US 8, 51 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
From rickf-at-desktopvisuals.com Fri Nov 22 04:42:43 2019 Return-Path: {rickf-at-desktopvisuals.com} Received: from adk-arts.com ([1.33.191.129]) by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id xAMAggu9015607; Fri, 22 Nov 2019 04:42:43 -0600 Message-Id: {201911221042.xAMAggu9015607-at-microscopy.com} Received: from User (unknown [91.110.31.15]) by adk-arts.com (Postfix) with ESMTPA id B162A97B788; Wed, 23 Oct 2019 10:11:08 +0900 (JST) Reply-To: {draymndch-at-yahoo.co.jp}
X-from: jhyun-at-gatan.com
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Email: jhyun-at-gatan.com Name: John Hyun
Organization: Gatan
Title-Subject: [Filtered] European EELS & EFTEM School Graz 2020
Message: February 4-7, 2020 FELMI-ZFE, Graz University of Technology Graz, Austria
Problem Solving with TEM
This premiere EELS training program will utilize the state of the art facilities at FELMI-ZFE including a monochromated probe-corrected Titan (S)TEM with a DualEELS GIF Quantum EELS system. The EELS spectrometer features a unique direct-electron detection K2 camera for low-noise, dose efficient applications in imaging and spectroscopy. Our qualified staff will familiarize you with the latest EELS & EFTEM equipment and will teach you fundamental principles and methods which are crucial to take top quality EELS spectra, STEM-EELS spectrum images and energy-filtered images or elemental maps. While not a focus of the workshop, optimization of the source monochromator for high-resolution EELS and the Cs probe corrector for STEM-EELS is included in the program. You will learn to apply practical techniques, how to use hardware and software systems as well as advanced EELS and EFTEM applications in a very efficient manner. The techniques are applicable to fields ranging from biological to materials research. Prior experience with transmission electron microscopy is beneficial, in the conventional and in the scanning (STEM) mode; a basic familiarity with EELS and EFTEM is an advantage.
For more information on the program, facilities and registration, please visit: https://www.felmi-zfe.at/teaching/lll-courses/european-eels-eftem-school/
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From tammyhoward072mxyve-at-gmail.com Tue Nov 26 18:29:24 2019 Return-Path: {tammyhoward072mxyve-at-gmail.com} Received: from gmail.com (a3.sk1oqu.cn [23.228.73.188] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id xAR0TNOh020980 for {microscopylistserverarchive7-at-microscopy.com} ; Tue, 26 Nov 2019 18:29:23 -0600 Received: from [195.37.235.214] by rly04.hottestmile.com with NNFMP; Tue, 26 Nov 2019 16:44:41 +0000 Received: from [65.5.209.127] by smtp.endend.nl with NNFMP; Tue, 26 Nov 2019 16:38:09 +0000 Message-ID: {1D0694C8.2346FE0B-at-gmail.com}
X-from: nasadi-at-ece.ufl.edu
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Title-Subject: [Filtered] IEEE PAINE 2020, July 28-29
Message: http://paine-conference.org/
Dear colleagues,
This is to inform you that the “IEEE International conference on Physical Assurance and Inspection on Electronics” (PAINE), is to be held on July 28-29, 2020 in Washington, DC. PAINE is supported by “Reliability Society” and accepted papers will be published in IEEE Xplore after peer review by the PAINE TPC. PAINE offers an exciting venue to researchers and practitioners in the field of Hardware Security and Trust who research and develop physical assurance and analysis. PAINE brings together experts from government, instrumentation, original component manufacturers (OCMs), original equipment manufacturers (OEMs), and academic researchers together to share ideas and solutions to make electronics devices and systems safe and secure. Topics covered are including but not limited to: • Image analysis and artificial intelligence for assurance and inspection • Novel material and devices for assurance • Sample Preparation • PCB trust and assurance • Chip and PCB level decomposition for assurance • Side channel assessment (power, timing, EM) for assurance and countermeasures • FIB/SEM for assurance • Electro-optical probing using PEM, EOP, EOFM, etc. • Physical/side channel fingerprinting • Mod-chip on PCB • Microprobing and nanoprobing • Bus-snooping • Fault injection assessment and countermeasures • Field-based weakness • Countermeasures against tampering and decomposition • Physical/logical shielding, etc. • FPGA Bitstream protection and vulnerabilities • Analog & mixed-signal circuits and systems security • Emerging topics in physical inspection and assurance • Security primitives: Novel devices, materials, and systems • Trojans and backdoors: Detection and prevention • Counterfeit Detection and Anti-Counterfeit Technique
SCHEDULE Full Paper (5 pages): Notification of Acceptance: Camera ready version: March 11, 2020 April 28, 2020 May 17 , 2020 CONTACT INFORMATION Technical Program: Navid Asadi (Program Chair) University of Florida E-mail: nasadi-at-ece.ufl.edu General Information: Mark Tehranipoor (General Chair) University of Florida E-mail: tehranipoor-at-ece.ufl.edu This event is open to public; we encourage everyone from academia, industry, and government to attend. Best Regards, PAINE organizing committee
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Email: tamavlyutov-at-wisc.edu Name: Tim
Organization: UW Madison
Title-Subject: [Filtered] to make sections flat
Message: Hello. My goal to cut 3 micron thick sections of mouse eyeballs from EPON embedded block and to arrange them flat on a glass slide surface. Later my goal is to reglue sectons to EPON block and to cut them 90 NM thin for EM. The problem with 3 micron thick sections is that after arranging them on a water drop and drying on a glass slide, their surface is not even. It is wavy, which gives me trouble to recut big area of interest with thin sections. Tissue is incomplete. Question is: how can I dry these sections to make them maximally flat? Something to add to water? To dry it some special way? Please let me know.
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X-from: Francesco Pasqualini {francesco.s.pasqualini-at-gmail.com}
All, I am leaving Boston (sad) to set up my laboratory in Italy and looking for a high-end microscope for live imaging of 2D and 3D preparations (10-500 um thick) of cardiac engineered tissues.
I have a budget of ~400k eur and a few conflicting requirements that make it difficult to identify the perfect system. In details:
* The main experiments will be imaging relatively slow processes (cell proliferation, migration, and maturation) in 2D/3D engineered tissues, ideally for 24-48 hrs across multiple FOC and multiple wells. Most confocal systems with environmental chambers will do the job here * The second type of experiment we will be performing with high frequency is the measurement of fast physiological signals in these cells, including calcium transients and propagation and traction force microscopy (~100 fps). For this application, most spinning disk confocal will work nicely and the large imaged area offered by the 25mm chip in the Nikon/Crest is interesting * I would also like the system to be capable of novel modalities, including super-resolution microscopy and light-sheet microscopy. Yokogawa/SORA or SIM setups might work for super-resolution and I am looking to the QuVi from Luxendo for light sheet microscopy
Ideally, one would get two separate systems but I rather have one good system in the budget than 2 mediocre ones. What do you gals/gents think? Any recommendations based on personal experience?
OK all you fiber and fabric microscopist, I got a question.
I'm examining a woven fabric were the warp and weft is not at 90 degrees to each other. I have no idea how this is done, but how do you describe this weave? It looks like a plain weave, one under and then over in both directions. My limited fiber and cloth resources don't describe the fabric? Any references or thoughts for me.
Thanks!
Stay safe...........
Frank Karl Microscopist Akron Rubber Development Laboratory 2887 Gilchrist Road Akron, Ohio 44305
I have a colleague who studied chloroplasts in sea slugs here in Hawaii. She had no problem identifying the chloroplasts in resin sections. For this there was no reason to make sure the chlorophyll was retained, only the chloroplasts. And with proper fixation they should stay there.
Aloha, Tina
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Email: Hui_Yang1-at-uml.edu {mailto:Hui_Yang1-at-uml.edu} Name: Hui Yang
Organization: University of Massachusetts Lowell
Title-Subject: [Filtered] Retain Chlorophyll in Sections
Message: Hello everyone, I am studying an animal-algae symbiosis. The algae invades animal tissue and has chlorophyll so I can usually see them in fixed animals using a fluorescence microscope. However, now I need to make some thin sections to get a closer look and most dehydration and infiltration procedures would wash away the chlorophyll. Does anyone know any embedding material that may be able to retain the chlorophyll? My specimens are 5-8 mm in length but I can cut out small pieces of 1-2 mm. The goal is to distinguish the algae from animal cells. I am hoping to cut 5-20 um sections, either paraffin or resin is fine. Thanks Hui
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-- Tina Weatherby Carvalho
University of Hawaii Pacific Biosciences Research Center Biological Electron Microscope Facility 2538 McCarthy Mall Honolulu, HI 96822 808-956-6251
Dear Hui, Here is a brute force solution. You could cut pieces small enough for high-pressure freezing, freeze, then replace the water in the specimen by freeze-substitution, infiltrate with resin at the low temperature, then cut sections. You would need to be near all the necessary equipment. There might be far easier ways. Yours, Bill
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The algae invades animal tissue and has chlorophyll so I } can usually see them in fixed animals using a fluorescence microscope. However, now I need to make } some thin sections to get a closer look and most dehydration and infiltration procedures would wash } away the chlorophyll. Does anyone know any embedding material that may be able to retain the } chlorophyll? My specimens are 5-8 mm in length but I can cut out small pieces of 1-2 mm. The goal is } to distinguish the algae from animal cells. I am hoping to cut 5-20 um sections, either paraffin or } resin is fine. } Thanks } Hui } } } Login Host: 209.222.216.34 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 10, 53 -- From microscopy.listserver-at-gmail.com Thu Nov 21 06:51:01 2019 } 10, 53 -- Received: from mail-io1-f68.google.com (mail-io1-f68.google.com [209.85.166.68]) } 10, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id xALCp1Hc008574 } 10, 53 -- for {microscopy-at-microscopy.com} ; Thu, 21 Nov 2019 06:51:01 -0600 } 10, 53 -- Received: by mail-io1-f68.google.com with SMTP id r2so3229926iot.10 } 10, 53 -- for {microscopy-at-microscopy.com} ; Thu, 21 Nov 2019 05:00:04 -0800 (PST) } 10, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 10, 53 -- d=gmail.com; s=20161025; } 10, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 10, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 10, 53 -- bh=j4EIyRGGJ+neZBenAGJJwVDU9HDlZkbIHLcSSHWnLig=; } 10, 53 -- b=QqX/9LNYDKY6uEHcGz03uhtogPOS7lx7TMc1dxwPNrI6fJcOMz2r3JrTfjWs7/zrqJ } 10, 53 -- ZT0kEQXQnNzC8yzoxl0nm8sXZ9w9xtgNR/LXDECa1esPUlN+YxMj+rvJ6XnLCWJUBa1Y } 10, 53 -- cF0vyCjsfGpFZBOPkUmFhDvzm5T20A8BqE9nhkUkTaVb0tp3mQnMs1FRqYwwUND9nqhL } 10, 53 -- /NtK0M0VlBU1l8bv7HHA/UG/QA5Hn0RGxHXKUoFQHKPqZrZaF4sarWhar1kqfgNnCETd } 10, 53 -- GIJMvZ17yT2Vd8znmAZ3azyrErG+/xkLnd+ujkMN86XgTRaKtqs/FStZFds/bS6hsP+P } 10, 53 -- CW4Q== } 10, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 10, 53 -- d=1e100.net; s=20161025; } 10, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 10, 53 -- :user-agent:mime-version:in-reply-to:content-language } 10, 53 -- :content-transfer-encoding; } 10, 53 -- bh=j4EIyRGGJ+neZBenAGJJwVDU9HDlZkbIHLcSSHWnLig=; } 10, 53 -- b=QTPhAnRHM4ZEu61mmcjh8JcB1hRWCmEOjXA01Ex2YZ5tbjBhdt1fd76jm3A+PnqPM0 } 10, 53 -- v0H8d/EhKceABcC34//ho0mbprQJnOAXjQJshbvTVKMVlRCAte00HWvBJLEQUdQj6sBK } 10, 53 -- 3w23C6p/rbu3E8vApTNTcWBvULfeRRTn5+eV6ieBnPha4mnDbPJb1uAR7nTrkr0UqQwu } 10, 53 -- hnllWv8PFOM1TtFlWy+Zder6bihvC/hZf/ZtbyjGClQYTe1k6gMEL7499Mm6QBgVfoa6 } 10, 53 -- 3dneesUerpsZueJvfNMsVWUauwft27AeqPPSdUUfHEoZQ3e7l18lSjLuziUX+E9sMuwn } 10, 53 -- 5/5A== } 10, 53 -- X-Gm-Message-State: APjAAAXA039/sY4sWA485yJH3F1TzbhlM+ULbCn4jzXVh1Lyi+eU6eva } 10, 53 -- lXIXSCOgXkyxAmEXw4xbxxFWgC83 } 10, 53 -- X-Google-Smtp-Source: APXvYqxajiRrs3cb87kkJSXPgP+X9Ofvc9KmbaVYVjQvs6FImmFhnb6uwWZlrqqtSOk2kfEzKBrJ7w== } 10, 53 -- X-Received: by 2002:a6b:d10c:: with SMTP id l12mr3430082iob.82.1574341203820; } 10, 53 -- Thu, 21 Nov 2019 05:00:03 -0800 (PST) } 10, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:5833:991e:4b53:455d]) } 10, 53 -- by smtp.googlemail.com with ESMTPSA id l64sm1151243ilh.2.2019.11.21.05.00.03 } 10, 53 -- for {microscopy-at-microscopy.com} } 10, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 10, 53 -- Thu, 21 Nov 2019 05:00:03 -0800 (PST) } 10, 53 -- Subject: viaWWW:Retain Chlorophyll in Sections } 10, 53 -- References: {201911202229.xAKMT1EA003476-at-microscopy.com} } 10, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 10, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 10, 53 -- X-Forwarded-Message-Id: {201911202229.xAKMT1EA003476-at-microscopy.com} } 10, 53 -- Message-ID: {33cb27c7-f0d4-13dd-54d4-f53dd65408a3-at-gmail.com} } 10, 53 -- Date: Thu, 21 Nov 2019 07:00:02 -0600 } 10, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.14; rv:60.0) } 10, 53 -- Gecko/20100101 Thunderbird/60.9.1 } 10, 53 -- MIME-Version: 1.0 } 10, 53 -- In-Reply-To: {201911202229.xAKMT1EA003476-at-microscopy.com} } 10, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 10, 53 -- Content-Language: en-US } 10, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
From polafutj1shaw-at-gmail.com Fri Nov 29 12:57:19 2019 Return-Path: {polafutj1shaw-at-gmail.com} Received: from gmail.com (a5.p8y9u4.cn [23.228.73.182] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id xATIvIju001114 for {microscopylistserverarchive7-at-microscopy.com} ; Fri, 29 Nov 2019 12:57:19 -0600 Message-ID: {E9D8B69D.4A3C98DD-at-gmail.com}
Dear Tim, your sectioning/flattening problem sounds bad… Guessing, you’ve finished your specimen preparation already (i.e. not in the stage of testing an optimal processing schedule) and so all your mouse eye balls are embedded already in one and the same Epon resin.
Eyeball(s) ‚as a whole‘ reportedly [check the MSA-Listserver archives and eventually also .Histonet-Listserver archives for keyword: | eye | or | eyeball |] v e r y difficult to embed for proper and easy sectioning…(homogenous „hardness“ of tissue in terms of optimal fixation – dehydration – finally resin penetration and polymerization often cannot be achieved….).
Parameters (besides/except proper fixation-dehydration) Embedding might be the
- type of dehydration (solvents-liquid: Ethanol, acetone,?) and the particular dehydration steps (changes and time) into the last concentrated solution - intermediate steps (i.e. duration of each change between absolute alcohol (or acetone) and infiltration - composition (mixing formula) of the embedding resin (soft - hard) itself, - infiltration steps done including transition media/intermediate fluids (i.e. replacing any former -traces of - solvent like EtOH or acetone or ??, which still might be trapped yet within the tissue) - modus of whole polymerization process (rapid versus 'slow' polymerization.
Sectioning: - type of knife used (and - naturally - the cutting parameters you still adjust yourself), guessing you use a diamond knife - deformation of section by (uneven) compression due to poor knife edge (if glass knife, or incorrect cutting parameters, especially cutting speed) as well as different "hardness=relative density" within the specimen block (BTW: what are the dimensions of your resin block-face to be sectioned?) - height of water in knife-'trough boat' (too high) - time for stretching on the water surface too short ( I know this will lengthen/ extend the whole section session....your sitting at the [ultra-]microtome) - complicated transfer of uneven and large sections from trough to object slide
Flattening of sections: - transfer unto a 'flat' waterdrop (due to low surface tension) and then 'allowing to air-dry' on the glass-slide most of the time is insufficient to get flat /wrinkle-free sections - it would be easy to use silanized or other adhesive-coated object-slides to i) produce "roundish, spherical" drops of water (CAVE you have to have in mind that finally you need to remove your re-glued 3 µm thick "semi-thin/thick sections" from the glass slide ...which might be complicated using the specially coated slides.... - I once used home-made, cleaned object slides coated with teflon spray -leaving empty small circles - treated with heat and then used for placing the /waterdrop(s) into these circles (tightly filled, therefore almost perfectly spherical). Perhaps uch modified object slides are available from specialized LM-/ EM-Suppliers - again then: drying step: transfer section (what do you use for that step? I used small, self made glass rods with - differently sized - beads) onto a sufficiently big spherical water drop ("surface volume/area" depends on the final dimensions of your section) - Don't use a hotplate for drying - if you can't control the hotplate by centigrades in range [35-50] degr. C. - Otherwise (if still at hand), use the heat emitted from a light bulb/filament lamp, eg. 60W-75W) positioned some 10-15 cm above the object slide. LET DRY SLOWLY (eventually add once to thrice a small volume of A. bidest - prewarmed to approx. 60 degr. C or 'freshly' from the still - by means of a syringe / clean =oil free = free from silicone oil - injection needle. I neither have made successful experiences with using chloroform vapours and other chemical vapors nor used a so-called "heat-pen" with regard to flattening sections well... Unfortunately - as long as I was benchworking in our EM-Lab - i didn't read (e.g., from specialized EM-tech-literature) any article dealing with additives to water for proper / better flattening of sections.
-(Guessing you'll stain your sections, then document morphology by dig. Photography and marking the region of interest to perform ) sectioning ultrathin -at- 70-90 nm for further EM-observation.... you will have to consider a proper technique to get off our 3µm sections from the slides: any thoughts? (POP-OFF technique etc.)
Disclaimer: Admitting to have done a lot of "Re-Embedding" techniques with regard to large diagnostic and experimental human tissue specimens (Pre-embedding ImmEM) and therefore having saved some experience and knowlegde about into retirement (:-)) Further tipps and hints - if interested - as well as specific literature on request
As always, respectfully but cordially,
Wolfgang H. Muss SALZBURG-AUSTRIA
============================================= MUSS Wolfgang Dr. phil./PhD [OR i. R./retired]
FRMS, Retired Member of MSA & other (Inter-)National Societies
Former Head of Electron Microscopy Lab at Institute of Pathology SALK-LKH / Salzburger Landeskliniken | General Hospital and PMU (private) PARACELSUS MEDICAL UNIVERSITY of SALZBURG
'Scientific' Profile including 'publications' at ResearchGate: http://www.researchgate.net/profile/Wolfgang_MUSS inviting you to join ResGate. (Sign up - ResearchGate -at- https://www.researchgate.net/signup.SignUp.html, and join 14+ million researchers, including 63 Nobel Laureates)
============================================================================ ===== Von: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Gesendet: Freitag, 29. November 2019 16:04 An: wij.muss-at-aon.at Betreff: [Microscopy] How to make sections flat
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Hello. My goal to cut 3 micron thick sections of mouse eyeballs from EPON embedded block and to arrange them flat on a glass slide surface. Later my goal is to reglue sectons to EPON block and to cut them 90 NM thin for EM. The problem with 3 micron thick sections is that after arranging them on a water drop and drying on a glass slide, their surface is not even. It is wavy, which gives me trouble to recut big area of interest with thin sections. Tissue is incomplete. Question is: how can I dry these sections to make them maximally flat? Something to add to water? To dry it some special way? Please let me know.
- using clean slides (ultrasonic bath, agressive cleaner, extensive rinsing in destilled water afterwards , drying afterwards) or use special and exĂĽensive adhesive slides "Superfrost" over here in europe
- make circle(s) with PAP-pen on slide
- put one big drop of destilled water into circle =--| ! the trick is to use "degassed" water, i.e. fresh (still warm) from the destill (not ion exchange water) or -alternatively- degass by sonicating a small amount of destilled water. My theory is that by heating the untreated water gas bubbles will form beneath the section. For many years I put acetone up to a final concentration of 20% to it, I thought it helped ...
- put section (e.g. with a fine tweezer, when cutting with a glass knife without water, or when using e.g. a semithin cutting diamond knife, fish with the tip of a pasteur pipette whose tip has been molten down to a small "ball" ) on droplet and then on a precision (!) heating plate, not (!) on a standard lab-heating plate, at 60 - 64° Celsius, higher temperatures will result in wrinkles ....
- let dry thoroughly, this can take some time but you see it easily by reflection / interference
I got quite a number of ultrathin sections from a 2.5 mikrometer section. If you do this regularly you can contact me, I used quite some nice tricks for re-gluing and cutting semithin sections for the transmission microscope..
best regards,
Peter ( Heimann )
( formerly Cell Biology, Bielefeld, Germany)
+++++++++++++++++++++++++++++++++++++++++
==============================Original Headers============================== 13, 20 -- From peter.heimann-at-uni-bielefeld.de Sat Nov 30 09:43:54 2019 13, 20 -- Received: from smtp.uni-bielefeld.de (uhrz-exch-pmb10.ad.uni-bielefeld.de [129.70.208.136]) 13, 20 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id xAUFhr7i004285 13, 20 -- for {Microscopy-at-microscopy.com} ; Sat, 30 Nov 2019 09:43:54 -0600 13, 20 -- Received: from [192.168.178.21] (79.210.50.84) by 13, 20 -- uhrz-exch-pmb10.ad.uni-bielefeld.de (129.70.208.136) with Microsoft SMTP 13, 20 -- Server (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_128_GCM_SHA256) id 13, 20 -- 15.1.1847.3; Sat, 30 Nov 2019 15:40:30 +0100 13, 20 -- To: {Microscopy-at-microscopy.com} 13, 20 -- From: pheimann {peter.heimann-at-uni-bielefeld.de} 13, 20 -- Subject: .... flat sections 13, 20 -- Message-ID: {f95bb671-3e27-3fa9-66c6-0c44e9c23614-at-uni-bielefeld.de} 13, 20 -- Date: Sat, 30 Nov 2019 15:40:29 +0100 13, 20 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:60.0) Gecko/20100101 13, 20 -- Thunderbird/60.9.1 13, 20 -- MIME-Version: 1.0 13, 20 -- Content-Type: text/plain; charset="utf-8"; format=flowed 13, 20 -- Content-Transfer-Encoding: 8bit 13, 20 -- X-ClientProxiedBy: uhrz-exch-pmb03.ad.uni-bielefeld.de (129.70.208.129) To 13, 20 -- uhrz-exch-pmb10.ad.uni-bielefeld.de (129.70.208.136) ==============================End of - Headers==============================
In the fiber composites business, there are "crowsfoot" (or "crows foot" or "crowfoot") fabric weaves in which the fill or weft yarns are at angles to the warp.
-------Roy
==============================Original Headers============================== 3, 17 -- From arrowood-at-zianet.com Sat Nov 30 16:44:12 2019 3, 17 -- Received: from oreo.zianet.com (oreo.zianet.com [216.223.228.104]) 3, 17 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id xAUMiBpV030019 3, 17 -- for {microscopy-at-microscopy.com} ; Sat, 30 Nov 2019 16:44:11 -0600 3, 17 -- Received: (qmail 33504 invoked by uid 0); 30 Nov 2019 21:40:49 -0000 3, 17 -- Received: from c-73-26-236-113.hsd1.nm.comcast.net (HELO ?192.168.0.2?) (arrowood-at-73.26.236.113) 3, 17 -- by zianet.com with ESMTPA; 30 Nov 2019 21:40:49 -0000 3, 17 -- To: microscopy-at-microscopy.com 3, 17 -- From: R Arrowood {arrowood-at-zianet.com} 3, 17 -- Subject: Re: question on fabrics 3, 17 -- Message-ID: {ff2a87e6-9efa-3dea-11aa-72695fd4d3de-at-zianet.com} 3, 17 -- Date: Sat, 30 Nov 2019 14:40:48 -0700 3, 17 -- User-Agent: Mozilla/5.0 (Windows NT 6.3; WOW64; rv:45.0) Gecko/20100101 3, 17 -- Thunderbird/45.8.0 3, 17 -- MIME-Version: 1.0 3, 17 -- Content-Type: text/plain; charset=utf-8; format=flowed 3, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
After thinking a bit (which I should have done before my first, incorrect answer), I remember that "crowsfoot" weaves do not involve yarns that are not orthogonal to each other. As far as I know, there is no concise name for the nonorthogonal fabrics used in composites. They are usually described as something like "2d biaxial nonorthogonal braided preforms", or "2d triaxial braids" if the fill or weft yarns are at both plus and minus theta to the warp.
I'm sorry that I've triggered such a firestorm of "out-of-office" autoreplies, not once, but twice!
-------Roy
==============================Original Headers============================== 4, 17 -- From arrowood-at-zianet.com Sat Nov 30 18:21:42 2019 4, 17 -- Received: from oreo.zianet.com (trinculo.zianet.com [216.223.228.124]) 4, 17 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id xB10LgP1009111 4, 17 -- for {microscopy-at-microscopy.com} ; Sat, 30 Nov 2019 18:21:42 -0600 4, 17 -- Received: (qmail 17324 invoked by uid 0); 30 Nov 2019 23:18:20 -0000 4, 17 -- Received: from c-73-26-236-113.hsd1.nm.comcast.net (HELO ?192.168.0.2?) (arrowood-at-73.26.236.113) 4, 17 -- by zianet.com with ESMTPA; 30 Nov 2019 23:18:20 -0000 4, 17 -- To: microscopy-at-microscopy.com 4, 17 -- From: R Arrowood {arrowood-at-zianet.com} 4, 17 -- Subject: Re: question on fabric weaves--a better answer 4, 17 -- Message-ID: {0174843f-68f5-7c7b-1e4d-4760d57951ae-at-zianet.com} 4, 17 -- Date: Sat, 30 Nov 2019 16:18:19 -0700 4, 17 -- User-Agent: Mozilla/5.0 (Windows NT 6.3; WOW64; rv:45.0) Gecko/20100101 4, 17 -- Thunderbird/45.8.0 4, 17 -- MIME-Version: 1.0 4, 17 -- Content-Type: text/plain; charset=utf-8; format=flowed 4, 17 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
From tammyhoward072syciv-at-gmail.com Sun Dec 1 22:19:13 2019 Return-Path: {tammyhoward072syciv-at-gmail.com} Received: from gmail.com (a6.4sa0c08.cn [23.228.73.171] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id xB24JC1L012595 for {microscopylistserverarchive7-at-microscopy.com} ; Sun, 1 Dec 2019 22:19:13 -0600 Received: from unknown (HELO smtp.doneohx.com) (Sun, 01 Dec 2019 11:20:53 -0800) by public.micromail.com.au with QMQP; Sun, 01 Dec 2019 11:20:53 -0800 Received: from unknown (67.243.51.33) by smtp-server1.cfdenselr.com with ESMTP; Sun, 01 Dec 2019 11:14:09 -0800 Received: from unknown (HELO rly04.hottestmile.com) (Sun, 01 Dec 2019 11:01:52 -0800) by qrx.quickslick.com with SMTP; Sun, 01 Dec 2019 11:01:52 -0800 Received: from relay.2yahoo.com ([137.169.237.12]) by smtp-server1.cfdenselr.com with SMTP; Sun, 01 Dec 2019 10:47:31 -0800 Message-ID: {871FAD1F.63ADD7D6-at-gmail.com}
FACULTY POSITION IN MATERIALS AT DREXEL UNIVERSITY
The Department of Materials Science & Engineering at Drexel University (www.drexel.edu/materials) is seeking applications for a tenured/tenure-track faculty position with a demonstrated record of excellence in original research in the general field of materials. Primary consideration will be given to candidates with areas of expertise in microscopy, metallurgy, and/or materials computation, although excellent candidates in all materials-related fields are encouraged to apply. Located in an exciting urban environment, our department has rapidly expanded during the past 10 years; consisting of 14 faculty, ~130 undergraduate and over ~80 graduate students working in four core research directions including nanomaterials, soft and biomaterials, electronic materials, and materials for energy and environment applications. We seek candidates who can establish a dynamic collaborative materials research program.
From dysonkizz7quvli-at-gmail.com Tue Dec 3 11:52:22 2019 Return-Path: {dysonkizz7quvli-at-gmail.com} Received: from gmail.com (a3.isbfbf.cn [23.228.73.189] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id xB3HqLOu006623 for {microscopylistserverarchive7-at-microscopy.com} ; Tue, 3 Dec 2019 11:52:22 -0600 Message-ID: {955ACDDF.42B7BF39-at-gmail.com}
Hi All, I am trying to get a Photonics Instruments Mosaic Digital Diaphragm System system working on a Nikon TI Eclipse microscope. In this configuration the system is controlled by a macro run from inside Nikon Elements software. I have tried versions of Elements from 3.1 up to 4.1. The computer is running Windows 7. These units are used, amongst other things, for FRAP and consist of a 2D array of mirrors. They work by selectively activating patterns in the array, so regions of a sample can be bleached for FRAP. The problem I am having is, I can either activate the whole array or none of the array, but not a pattern in the array. Has anybody encountered a similar problem and know a solution or way of troubleshooting. Alternately, is there any standalone software that could test the system outside of Elements. I looked at the list of supported devices for Micro-Manager and can see " Andor Mosaic DMD Projection Device". Which is a later similar device (Andor possibly bought out Photonics Instruments), but not the device I have?
Regards Lloyd
Director Bio-Imaging & Network Core Facilities Hunter College, City University of New York Department of Biological Sciences Rm. 826 HN 695 Park Ave New York, NY 10065 212 650 3872; fax: 212 650-3656
==============================Original Headers============================== 5, 27 -- From Williams-at-GENECTR.HUNTER.CUNY.EDU Tue Dec 3 15:09:50 2019 5, 27 -- Received: from gcmail.hunter.cuny.edu (genemx.hunter.cuny.edu [146.95.150.49]) 5, 27 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id xB3L9oT3018665 5, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 3 Dec 2019 15:09:50 -0600 5, 27 -- Received: from BIOHELPDESK.bio.hunter.cuny.edu (146.95.150.36) by 5, 27 -- genemx.bio.hunter.cuny.edu (146.95.150.49) with Microsoft SMTP Server (TLS) 5, 27 -- id 14.3.468.0; Tue, 3 Dec 2019 15:06:37 -0500 5, 27 -- Received: from gcmail.bio.hunter.cuny.edu ([169.254.1.179]) by 5, 27 -- BioHelpDesk.bio.hunter.cuny.edu ([146.95.150.36]) with mapi id 5, 27 -- 14.02.0387.000; Tue, 3 Dec 2019 15:06:37 -0500 5, 27 -- From: Lloyd Williams {Williams-at-GENECTR.HUNTER.CUNY.EDU} 5, 27 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 5, 27 -- Subject: Issue With Photonics Instruments Mosaic Digital Diaphragm System 5, 27 -- Thread-Topic: Issue With Photonics Instruments Mosaic Digital Diaphragm 5, 27 -- System 5, 27 -- Thread-Index: AdWqElnType0JwYyS6mjes1E68n3Ug== 5, 27 -- Date: Tue, 3 Dec 2019 20:06:36 +0000 5, 27 -- Message-ID: {82442657AE7F624586F6FCBFF78E8040013AE05BF2-at-gcmail.bio.hunter.cuny.edu} 5, 27 -- Accept-Language: en-US 5, 27 -- Content-Language: en-US 5, 27 -- X-MS-Has-Attach: 5, 27 -- X-MS-TNEF-Correlator: 5, 27 -- x-originating-ip: [146.95.148.144] 5, 27 -- Content-Type: text/plain; charset="iso-8859-1" 5, 27 -- MIME-Version: 1.0 5, 27 -- Content-Transfer-Encoding: 8bit 5, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id xB3L9oT3018665 ==============================End of - Headers==============================
Join us for the webinar series on X-ray Computed Tomography for Materials Science to find out how X-ray CT can help your research.
When: December 11, 2019 11:00 AM Pacific Time Part 4: X-ray Computed Tomography for Materials Science: Foams and Composites Applications
In this webinar, you will learn: - Keys to high-resolution imaging - Foams applications - Composites applications
The webinar series will cover data collection and analysis techniques and a number of application examples of food, pharmaceutical, foam and composite materials, etc.
To learn more, visit: https://www.rigaku.com/en/webinars/x-ray_ct_introduction
Recording of the webinar will be available for registered attendees.
Hope to see you there.
Aya Takase . Senior Scientist Rigaku Americas Corporation 9009 New Trails Drive . The Woodlands, TX 77381 USA T: 281-362-2300 ex 208 . F: 281-364-3628
==============================Original Headers============================== 10, 54 -- From btv1==242d246f5bb==Aya.Takase-at-rigaku.com Thu Dec 5 17:40:22 2019 10, 54 -- Received: from spamfilter.rigaku.com (spamfilter.rigaku.com [146.20.167.112]) 10, 54 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id xB5NeMdO002630 10, 54 -- for {Microscopy-at-microscopy.com} ; Thu, 5 Dec 2019 17:40:22 -0600 10, 54 -- X-ASG-Debug-ID: 1575585437-0fdc514c0774aaf0001-1DjkGe 10, 54 -- Received: from 592200-Exch2.rac.local ([172.16.230.5]) by spamfilter.rigaku.com with ESMTP id TwZCBw9lWjmxf915 (version=TLSv1.2 cipher=ECDHE-RSA-AES256-SHA384 bits=256 verify=NO) for {Microscopy-at-microscopy.com} ; Thu, 05 Dec 2019 17:37:17 -0500 (EST) 10, 54 -- X-Barracuda-Envelope-From: Aya.Takase-at-rigaku.com 10, 54 -- X-Barracuda-RBL-Trusted-Forwarder: 172.16.230.5 10, 54 -- Content-Language: en-US 10, 54 -- Content-Type: text/plain; charset="iso-8859-1" 10, 54 -- DKIM-Signature: v=1; a=rsa-sha1; d=rigaku.com; s=dkim1; c=relaxed/relaxed; 10, 54 -- t=1575585437; h=from:subject:to:date:ad-hoc; 10, 54 -- bh=EnPwG6bRR+qRzjvWLj7vIbIcW08=; 10, 54 -- b=eMfmPmt+t5mbb4OsW7rB/8V53cPFxMZBI5zQlRodCPtZLstcg4XRk6wSQfCbc88NmjDqo3FeDuQ 10, 54 -- eGwcc8IgmH+MtrZVfZvCkQGw82+G48dFYN1I1XBVNB2rHOMxD3pS6/9zgcZ+qh7DlMEeqkzyDQE50 10, 54 -- 8EJ07T2bhUuxlMxE9Ey0kg6h/9vV+t0PoKR8uOmo0RHQWJ9mkH4N6zSJldTTH1IzlY+9xgxrUeh/o 10, 54 -- nktpaZiyLDgVFUFiz8tP5jnUZ88Gy/Ii0BflpQhIJOW9LZIHf8V2e4Ha2NXSv+c+2b27AGAq1rTED 10, 54 -- pGbrEmOkxtrekJhL04LWdPNo5bo/P8LZI0eg== 10, 54 -- Received: from 592200-EXCH2.rac.local (172.16.230.5) by 592200-Exch2.rac.local 10, 54 -- (172.16.230.5) with Microsoft SMTP Server (TLS) id 15.0.1365.1; Thu, 5 Dec 10, 54 -- 2019 17:37:16 -0500 10, 54 -- Received: from 592200-EXCH2.rac.local ([fe80::821:2890:77ec:aa4f]) by 10, 54 -- 592200-Exch2.rac.local ([fe80::821:2890:77ec:aa4f%16]) with mapi id 10, 54 -- 15.00.1365.000; Thu, 5 Dec 2019 17:37:16 -0500 10, 54 -- X-Barracuda-RBL-Trusted-Forwarder: 172.16.230.5 10, 54 -- From: Aya Takase {Aya.Takase-at-rigaku.com} 10, 54 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 10, 54 -- Subject: Webinar series: X-ray Computed Tomography for Materials Science 10, 54 -- Thread-Topic: Webinar series: X-ray Computed Tomography for Materials Science 10, 54 -- X-ASG-Orig-Subj: Webinar series: X-ray Computed Tomography for Materials Science 10, 54 -- Thread-Index: AdWq2LB3+J/5vAbNS4mcG/M0ehbSRwA48X+w 10, 54 -- Date: Thu, 5 Dec 2019 22:37:15 +0000 10, 54 -- Message-ID: {242c86a0a6b344829d529cd5574b7d3b-at-592200-Exch2.rac.local} 10, 54 -- Accept-Language: en-US 10, 54 -- X-MS-Has-Attach: 10, 54 -- X-MS-TNEF-Correlator: 10, 54 -- x-ms-exchange-transport-fromentityheader: Hosted 10, 54 -- x-originating-ip: [10.2.2.29] 10, 54 -- MIME-Version: 1.0 10, 54 -- X-Barracuda-Connect: UNKNOWN[172.16.230.5] 10, 54 -- X-Barracuda-Start-Time: 1575585437 10, 54 -- X-Barracuda-Encrypted: ECDHE-RSA-AES256-SHA384 10, 54 -- X-Barracuda-URL: https://spamfilter.rigaku.com:443/cgi-mod/mark.cgi 10, 54 -- X-Virus-Scanned: by bsmtpd at rigaku.com 10, 54 -- X-Barracuda-Scan-Msg-Size: 911 10, 54 -- X-Barracuda-BRTS-Status: 1 10, 54 -- X-Barracuda-Spam-Score: 0.00 10, 54 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using global scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=5.0 KILL_LEVEL=1000.0 tests= 10, 54 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.78479 10, 54 -- Rule breakdown below 10, 54 -- pts rule name description 10, 54 -- ---- ---------------------- -------------------------------------------------- 10, 54 -- Content-Transfer-Encoding: 8bit 10, 54 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id xB5NeMdO002630 ==============================End of - Headers==============================
From ralpzand8ijwe-at-gmail.com Sat Dec 7 05:31:58 2019 Return-Path: {ralpzand8ijwe-at-gmail.com} Received: from gmail.com (a6.l18l1.cn [23.228.73.187] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id xB7BVv6D005897 for {microscopylistserverarchive7-at-microscopy.com} ; Sat, 7 Dec 2019 05:31:58 -0600 Message-ID: {F8EFD23B.1698BE47-at-gmail.com}
X-from: Ning, Gang {gxn7-at-psu.edu}
Dear Colleagues,
A Leica 1950 cryostat in my lab recently has a problem with sample head cooling. The head, which holds the sample, cannot be cooled down to the set temperature so the sample melts during the sectioning process because a cooling module in the chip failed. To repair the head it costs over $8k but the company suggests that it can be bypassed by disactivating the head cooling module so that the temperature of the sample will stay the same as the chamber but no longer adjustable. My question is how important to have the sample head temperature adjustable for sectioning? Hope some of you out there can give me some advice.
From duranelizabethe2jm-at-gmail.com Wed Dec 11 13:06:42 2019 Return-Path: {duranelizabethe2jm-at-gmail.com} Received: from gmail.com (a3.ihpr68.cn [23.228.73.172] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id xBBJ6flZ030597 for {microscopylistserverarchive7-at-microscopy.com} ; Wed, 11 Dec 2019 13:06:42 -0600 Message-ID: {547EC10B.A2FDA7D0-at-gmail.com}
Hi everyone,
Do you know of a way to convert TVIPS formatted videos into something more easily readable like MKV or AVI? I’ve visited the company’s website but I can’t find any viewers or conversion tools.
Thank you!
______________________________________ Steven R. Spurgeon, Ph.D. Research Scientist Energy and Environment Directorate
Pacific Northwest National Laboratory 902 Battelle Boulevard P.O. Box 999 MSIN:P7-25 Richland, WA 99352
X-from: Sarah Holmes, Lab for Kidney Pathology {sarahlkp-at-comcast.net}
Hi Greg,
We have a 1950 that does not have the sample head cooling option activated. We bought it that way.
It works fine without to section at -27 and 2 microns.
Sarah
On 12/11/2019 8:51 AM, microscopy.listserver-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } } X-from: Ning, Gang {gxn7-at-psu.edu} } } } Dear Colleagues, } } A Leica 1950 cryostat in my lab recently has a problem with sample head cooling. The head, which } holds the sample, cannot be cooled down to the set temperature so the sample melts during the } sectioning process because a cooling module in the chip failed. To repair the head it costs over $8k } but the company suggests that it can be bypassed by disactivating the head cooling module so that } the temperature of the sample will stay the same as the chamber but no longer adjustable. My } question is how important to have the sample head temperature adjustable for sectioning? Hope some } of you out there can give me some advice. } } Thank you in advance! } } Greg } } Gang (Greg) Ning } } Microscopy Core Facility } } Huck Institutes of the Life Sciences } } Penn State University } } MSC N-048 } } University Park, PA 16802 } } 814-863-0994 } } https://www.huck.psu.edu/core-facilities/microscopy-facility } } } } } ==============================Original Headers============================== } 19, 53 -- From microscopy.listserver-at-gmail.com Wed Dec 11 08:36:51 2019 } 19, 53 -- Received: from mail-io1-f41.google.com (mail-io1-f41.google.com [209.85.166.41]) } 19, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id xBBEap0G018112 } 19, 53 -- for {microscopy-at-microscopy.com} ; Wed, 11 Dec 2019 08:36:51 -0600 } 19, 53 -- Received: by mail-io1-f41.google.com with SMTP id x1so22649420iop.7 } 19, 53 -- for {microscopy-at-microscopy.com} ; Wed, 11 Dec 2019 05:34:04 -0800 (PST) } 19, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 19, 53 -- d=gmail.com; s=20161025; } 19, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 19, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 19, 53 -- bh=Zwygux9eYbc0y6moy/Zbd3rdsCHBKM4Hi8wdQcJ2UOw=; } 19, 53 -- b=IU8+23pWlXoH3+zbq3jGJWegqnLXYmF7meudXJa/MqB2VIR+xCo1BBmPA1AKb3G7UA } 19, 53 -- iWAkvyM+lgps7xEkdgtMunen/R3P5V2AFpKRZSlqxxf8wfLjFmUF1W4ormQ5xsKhRcsZ } 19, 53 -- EAn93Tct5yvWDV8vXUSMlQ6tjN2tp+1b3FtZtqsfEOfrVdZ+m9K5mht65xfOkK7gFR6R } 19, 53 -- ciU/X7JW/ko+HYUlbPbhsbYPFaggZJe6w/Rj45PqvO/eZ6TPu2UjuNI0sXxl8UHB2eij } 19, 53 -- s1NC76Pdyc2vJxxNr5L5aiWGbVPfSl/9I3ke1KAot/g1/O/ZUIFjO/B9kwQk16hCit2R } 19, 53 -- jxYw== } 19, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 19, 53 -- d=1e100.net; s=20161025; } 19, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 19, 53 -- :user-agent:mime-version:in-reply-to:content-language } 19, 53 -- :content-transfer-encoding; } 19, 53 -- bh=Zwygux9eYbc0y6moy/Zbd3rdsCHBKM4Hi8wdQcJ2UOw=; } 19, 53 -- b=NKZFWwXJWIbFDTqEkaKDIoqjghsCGAzL7ZC4/XD+NJ/VWXeiAZV1lVNguPFDeXdgvM } 19, 53 -- wLJA9JpekcjPju1QW/MMT1IMGEAbW7GZM8xiajet4HXofTEjrrOWbXCve/KUcbR4iGF6 } 19, 53 -- RADWFlC0N+OGxZHIn9KeSQlXBq6x+jO0xvCy6aUOzddX/ZwuPGAnf7tjSYGpdipIZLfj } 19, 53 -- pIrW3+4SiKXvNy1GodE+KfFBDSoogAqnqR9kS0OpqnHmt4xc9I7frJz59NcV/4mXcLVX } 19, 53 -- RIIqeXQ8IoGdH/jw56aqlYCLD/81wgbaARvzyuf1RTVuTAKrlPZmKCTo6wFtDNsSNsmV } 19, 53 -- 62/g== } 19, 53 -- X-Gm-Message-State: APjAAAVeBQ8KmzN9RrGY+GYTif8zSmOeQeJnvQBByyxXertQKb1bTssq } 19, 53 -- Vez7sssxT2U3aGPgO1Zx7qrIibnA } 19, 53 -- X-Google-Smtp-Source: APXvYqxQ++TSxCn4CJwt61wvvwwMQSluWn7SyJ2FD+fZhkMkBNPCwlsbBV1T6+tMrRTu3d6YxF7ITQ== } 19, 53 -- X-Received: by 2002:a02:a492:: with SMTP id d18mr3174549jam.84.1576071243585; } 19, 53 -- Wed, 11 Dec 2019 05:34:03 -0800 (PST) } 19, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:7439:e72:ae6a:d239]) } 19, 53 -- by smtp.googlemail.com with ESMTPSA id t2sm521925iol.39.2019.12.11.05.34.02 } 19, 53 -- for {microscopy-at-microscopy.com} } 19, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 19, 53 -- Wed, 11 Dec 2019 05:34:03 -0800 (PST) } 19, 53 -- Subject: Fwd: Leica 1950 cryostat sample head cooling help } 19, 53 -- References: {DM5PR0201MB349465A323B3632671154EA6905B0-at-DM5PR0201MB3494.namprd02.prod.outlook.com} } 19, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 19, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 19, 53 -- X-Forwarded-Message-Id: {DM5PR0201MB349465A323B3632671154EA6905B0-at-DM5PR0201MB3494.namprd02.prod.outlook.com} } 19, 53 -- Message-ID: {8dc8499f-3832-f260-6ef5-02b68c196124-at-gmail.com} } 19, 53 -- Date: Wed, 11 Dec 2019 07:34:00 -0600 } 19, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.14; rv:60.0) } 19, 53 -- Gecko/20100101 Thunderbird/60.9.1 } 19, 53 -- MIME-Version: 1.0 } 19, 53 -- In-Reply-To: {DM5PR0201MB349465A323B3632671154EA6905B0-at-DM5PR0201MB3494.namprd02.prod.outlook.com} } 19, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 19, 53 -- Content-Language: en-US } 19, 53 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers============================== } -- Sarah Holmes Laboratory Manager Laboratory for Kidney Pathology, Inc. Nashville, TN 615 321 5729
Hi there in the old days, all cryostats had no sample temp. it was only chamber temp. it should work fine without the sample temp, all the best have a great holiday season. Mario
-----Original Message----- X-from: microscopy.listserver {microscopy.listserver-at-gmail.com} To: mocleica {mocleica-at-aol.com} Sent: Wed, Dec 11, 2019 4:39 pm
X-from: Â Â Â Ning, Gang {gxn7-at-psu.edu {mailto:gxn7-at-psu.edu} }
Dear Colleagues,
A Leica 1950 cryostat in my lab recently has a problem with sample head cooling. The head, which holds the sample, cannot be cooled down to the set temperature so the sample melts during the sectioning process because a cooling module in the chip failed. To repair the head it costs over $8k but the company suggests that it can be bypassed by disactivating the head cooling module so that the temperature of the sample will stay the same as the chamber but no longer adjustable. My question is how important to have the sample head temperature adjustable for sectioning? Hope some of you out there can give me some advice.
==============================Original Headers============================== 19, 53 -- From microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} Wed Dec 11 08:36:51 2019 19, 53 -- Received: from mail-io1-f41.google.com (mail-io1-f41.google.com [209.85.166.41]) 19, 53 -- Â Â Â by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id xBBEap0G018112 19, 53 -- Â Â Â for {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } ; Wed, 11 Dec 2019 08:36:51 -0600 19, 53 -- Received: by mail-io1-f41.google.com with SMTP id x1so22649420iop.7 19, 53 --Â Â Â Â for {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } ; Wed, 11 Dec 2019 05:34:04 -0800 (PST) 19, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 19, 53 --Â Â Â Â d=gmail.com; s=20161025; 19, 53 --Â Â Â Â h=subject:references:to:from:message-id:date:user-agent:mime-version 19, 53 --Â Â Â Â Â :in-reply-to:content-language:content-transfer-encoding; 19, 53 --Â Â Â Â bh=Zwygux9eYbc0y6moy/Zbd3rdsCHBKM4Hi8wdQcJ2UOw=; 19, 53 --Â Â Â Â b=IU8+23pWlXoH3+zbq3jGJWegqnLXYmF7meudXJa/MqB2VIR+xCo1BBmPA1AKb3G7UA 19, 53 --Â Â Â Â Â iWAkvyM+lgps7xEkdgtMunen/R3P5V2AFpKRZSlqxxf8wfLjFmUF1W4ormQ5xsKhRcsZ 19, 53 --Â Â Â Â Â EAn93Tct5yvWDV8vXUSMlQ6tjN2tp+1b3FtZtqsfEOfrVdZ+m9K5mht65xfOkK7gFR6R 19, 53 --Â Â Â Â Â ciU/X7JW/ko+HYUlbPbhsbYPFaggZJe6w/Rj45PqvO/eZ6TPu2UjuNI0sXxl8UHB2eij 19, 53 --Â Â Â Â Â s1NC76Pdyc2vJxxNr5L5aiWGbVPfSl/9I3ke1KAot/g1/O/ZUIFjO/B9kwQk16hCit2R 19, 53 --Â Â Â Â Â jxYw== 19, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 19, 53 --Â Â Â Â d=1e100.net; s=20161025; 19, 53 --Â Â Â Â h=x-gm-message-state:subject:references:to:from:message-id:date 19, 53 --Â Â Â Â Â :user-agent:mime-version:in-reply-to:content-language 19, 53 --Â Â Â Â Â :content-transfer-encoding; 19, 53 --Â Â Â Â bh=Zwygux9eYbc0y6moy/Zbd3rdsCHBKM4Hi8wdQcJ2UOw=; 19, 53 --Â Â Â Â b=NKZFWwXJWIbFDTqEkaKDIoqjghsCGAzL7ZC4/XD+NJ/VWXeiAZV1lVNguPFDeXdgvM 19, 53 --Â Â Â Â Â wLJA9JpekcjPju1QW/MMT1IMGEAbW7GZM8xiajet4HXofTEjrrOWbXCve/KUcbR4iGF6 19, 53 --Â Â Â Â Â RADWFlC0N+OGxZHIn9KeSQlXBq6x+jO0xvCy6aUOzddX/ZwuPGAnf7tjSYGpdipIZLfj 19, 53 --Â Â Â Â Â pIrW3+4SiKXvNy1GodE+KfFBDSoogAqnqR9kS0OpqnHmt4xc9I7frJz59NcV/4mXcLVX 19, 53 --Â Â Â Â Â RIIqeXQ8IoGdH/jw56aqlYCLD/81wgbaARvzyuf1RTVuTAKrlPZmKCTo6wFtDNsSNsmV 19, 53 --Â Â Â Â Â 62/g== 19, 53 -- X-Gm-Message-State: APjAAAVeBQ8KmzN9RrGY+GYTif8zSmOeQeJnvQBByyxXertQKb1bTssq 19, 53 -- Â Â Â Vez7sssxT2U3aGPgO1Zx7qrIibnA 19, 53 -- X-Google-Smtp-Source: APXvYqxQ++TSxCn4CJwt61wvvwwMQSluWn7SyJ2FD+fZhkMkBNPCwlsbBV1T6+tMrRTu3d6YxF7ITQ== 19, 53 -- X-Received: by 2002:a02:a492:: with SMTP id d18mr3174549jam.84.1576071243585; 19, 53 --Â Â Â Â Wed, 11 Dec 2019 05:34:03 -0800 (PST) 19, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:7439:e72:ae6a:d239]) 19, 53 --Â Â Â Â by smtp.googlemail.com with ESMTPSA id t2sm521925iol.39.2019.12.11.05.34.02 19, 53 --Â Â Â Â for {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } 19, 53 --Â Â Â Â (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 19, 53 --Â Â Â Â Wed, 11 Dec 2019 05:34:03 -0800 (PST) 19, 53 -- Subject: Fwd: Leica 1950 cryostat sample head cooling help 19, 53 -- References: {DM5PR0201MB349465A323B3632671154EA6905B0-at-DM5PR0201MB3494.namprd02.prod.outlook.com {mailto:DM5PR0201MB349465A323B3632671154EA6905B0-at-DM5PR0201MB3494.namprd02.prod.outlook.com} } 19, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } 19, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } 19, 53 -- X-Forwarded-Message-Id: {DM5PR0201MB349465A323B3632671154EA6905B0-at-DM5PR0201MB3494.namprd02.prod.outlook.com {mailto:DM5PR0201MB349465A323B3632671154EA6905B0-at-DM5PR0201MB3494.namprd02.prod.outlook.com} } 19, 53 -- Message-ID: {8dc8499f-3832-f260-6ef5-02b68c196124-at-gmail.com {mailto:8dc8499f-3832-f260-6ef5-02b68c196124-at-gmail.com} } 19, 53 -- Date: Wed, 11 Dec 2019 07:34:00 -0600 19, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.14; rv:60.0) 19, 53 --Â Gecko/20100101 Thunderbird/60.9.1 19, 53 -- MIME-Version: 1.0 19, 53 -- In-Reply-To: {DM5PR0201MB349465A323B3632671154EA6905B0-at-DM5PR0201MB3494.namprd02.prod.outlook.com {mailto:DM5PR0201MB349465A323B3632671154EA6905B0-at-DM5PR0201MB3494.namprd02.prod.outlook.com} } 19, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed 19, 53 -- Content-Language: en-US 19, 53 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both nizets2-at-yahoo.com, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Email: nizets2-at-yahoo.com
Name: Stephane
Organization: private
Title-Subject: [Filtered] Paraffin samples in SEM
Message: Dear colleagues, We would like to validate a method with SEM (meaning that another preparation method more suited to SEM cannot be considered) and this would require the analysis of paraffin blocks in SEM and I fear that this would melt the paraffin. Has any of you experience with observation of paraffin-embedded material in SEM? Optimally we would like to perform EDX analysis on the paraffin block surface, which would require enough kV to see Al and Si (I don't know the energy lines off the top of my head). Do you think that the electron beam could heat the paraffin up to the melting point ? Would it be possible to circumvent this by coating the sample with a "thick" layer of conductor? Best regards, Stephane
PS: I am a member of the listserver but I am too stupid to configure yahoo to send plain text messages instead of html messages so I have to use the online form.
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both becks-at-ncc.edu, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Email: becks-at-ncc.edu Name: Steve Beck
Organization: Nassau Community College
Title-Subject: [Filtered] Leica UC7 Knife Stage
Message: My UC7 knife stage is not rotating/pivoting using the two knobs on each side of the stage. I can rotate it by hand. I removed the bottom plate of the stage and can visualize the spiral gear that interfaces with a circular white plate with teeth. I was informed this is a common problem and it needs a tension adjustment? Does anyone know how to make this adjustment?
Thanks for any assistance!
Steve
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First, create a flat surface on the block of tissue. Preferably, you can section into the block with a standard microtome. Alternatively, carve the block down with a scalpel blade.
Second, remove the paraffin. You can do this using standard histological techniques, like removing the paraffin wax from paraffin sections. Xylene or a substitute will do it.
Third, transition gradually from xylene, through an ethanol series, to 100% ethanol.
Finally, critical point dry your tissue block, mount, and coat in the usually ways for SEM. View the flat or carved surface with the SEM.
I can't speak to EDS using this method.
--Best, Jan Factor
Jan Robert Factor, Ph.D. Professor of Biology, Chair of Biology Program Purchase College, State University of New York Purchase, NY 10577 Office: 2016 Natural Science Bldg., 914-251-6659 Office Hours (Fall 2019): Mon., 12:00-1:00, and Wed., 4:30-6:00 jan.factor-at-purchase.edu
Coral Reef Biology and Ecology Program: January in Roatan, purchase.edu/coralreef
Purchase College ranked in the Top 10 public liberal arts colleges nationally Please consider the environment before printing this e-mail
________________________________________ X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Wednesday, December 11, 2019 9:05 PM To: Factor, Jan
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both nizets2-at-yahoo.com, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Email: nizets2-at-yahoo.com
Name: Stephane
Organization: private
Title-Subject: [Filtered] Paraffin samples in SEM
Message: Dear colleagues, We would like to validate a method with SEM (meaning that another preparation method more suited to SEM cannot be considered) and this would require the analysis of paraffin blocks in SEM and I fear that this would melt the paraffin. Has any of you experience with observation of paraffin-embedded material in SEM? Optimally we would like to perform EDX analysis on the paraffin block surface, which would require enough kV to see Al and Si (I don't know the energy lines off the top of my head). Do you think that the electron beam could heat the paraffin up to the melting point ? Would it be possible to circumvent this by coating the sample with a "thick" layer of conductor? Best regards, Stephane
PS: I am a member of the listserver but I am too stupid to configure yahoo to send plain text messages instead of html messages so I have to use the online form.
Login Host: 213.33.126.84 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
On Thursday, December 12, 2019, 09:47:41 AM UTC, Stephane Nizet {nizets2-at-yahoo.com} wrote:
Hi Steve!
Last time it happened to me (after 10 years without any problem), I just needed to add grease and it was good for another 10 years :-) Worth a try, I'd say.
Regards, Stephane
On Thursday, December 12, 2019, 02:13:58 AM UTC, microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} wrote:
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both becks-at-ncc.edu, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Email: becks-at-ncc.edu Name: Steve Beck
Organization: Nassau Community College
Title-Subject: [Filtered] Leica UC7 Knife Stage
Message: My UC7 knife stage is not rotating/pivoting using the two knobs on each side of the stage. I can rotate it by hand. I removed the bottom plate of the stage and can visualize the spiral gear that interfaces with a circular white plate with teeth. I was informed this is a common problem and it needs a tension adjustment? Does anyone know how to make this adjustment?
Thanks for any assistance!
Steve
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Although I appreciate any hint to avoid the problem, I may not be able to = process the paraffin block very much like sectionning and dissolving becaus= e I preferentially need the full sample to be able to compare and correlate= with another method.Any treatment would require a new validation to ascert= ain that it does not impair the comparison and I would like to avoid that.F= or example, if I dissolve the paraffin I lose the position of the particles= . So my primary question remains open: will the paraffin melt under the elect= ron beam?I saw that Al and Si peaks are under 2keV in EDX, so using 5 kV sh= ould work.Would working in low vacuum possibly decrease the heating of the = sample? Would it still be possible to perform EDX analysis in low vacuum? Thank you in advance.Stephane
On Thursday, December 12, 2019, 01:50:32 AM UTC, xxxx wrote:
Hi Stephane If you have enough material, I suggest using paraffin sections.
When I did this type of EDS, the histologist placed a paraffin section on a carbon planchette. Then the paraffin was removed (dissolved). I don't remember if I critical point dried it, but Jan says do that. Maybe air drying is enough? Best wishes,
First, create a flat surface on the block of tissue. Preferably, you can section into the block with a standard microtome. Alternatively, carve the block down with a scalpel blade.
Second, remove the paraffin. You can do this using standard histological techniques, like removing the paraffin wax from paraffin sections. Xylene or a substitute will do it.
Third, transition gradually from xylene, through an ethanol series, to 100% ethanol.
Finally, critical point dry your tissue block, mount, and coat in the usually ways for SEM. View the flat or carved surface with the SEM.
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Email: nizets2-at-yahoo.com
Name: Stephane
Organization: private
Title-Subject: [Filtered] Paraffin samples in SEM
Message: Dear colleagues, We would like to validate a method with SEM (meaning that another preparation method more suited to SEM cannot be considered) and this would require the analysis of paraffin blocks in SEM and I fear that this would melt the paraffin. Has any of you experience with observation of paraffin-embedded material in SEM? Optimally we would like to perform EDX analysis on the paraffin block surface, which would require enough kV to see Al and Si (I don't know the energy lines off the top of my head). Do you think that the electron beam could heat the paraffin up to the melting point ? Would it be possible to circumvent this by coating the sample with a "thick" layer of conductor? Best regards, Stephane
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Yes, the paraffin will very likely melt under the beam. Even "hard" paraffin melts at 65 deg C, and the sample will be hotter than that where the beam impacts the sample. Besides melting the paraffin, this will re-arrange the sample's ultrastructure and likely cause the elements of interest to migrate. The paraffin will also weaken (by absorption) or possibly obscure the x-ray signal from the sample. Plus, it will contaminate the SEM's chamber and condense oil on the snout of the EDS detector (since it's cold). So, yes, you have to de-embed the sample. De-embedding can produce excellent results; I've done this with both sections and for internal anatomy of small crustaceans by hand-carving the critters while in paraffin.
Further note: You'll want to make sample holders with a hole in them, so the area of interest for EDS bridges the hole. This will decrease the x-ray background significantly. Drill a hole through a brass or (better) carbon/polymer cylinder. Coat with Aquadag or some such conductive carbon paste. (But do a background EDS on this - I've found high levels of phosphorus in some lots of Aquadag.) Keep in mind SEM stubs are almost all aluminum, so you'll get an aluminum x-ray signal whether or not there is Al in your sample. Control for this.
For Al and Si, you'll want to use 4-5 kV.
The other question is: how liable are Al and Si in your samples? For some elements in tissues, like Na, K, Mg, and the like, one has to use cryofixation/processing/cryoSEM, as these elements are lost otherwise during processing. This may be the case for Al and possible (though not likely) for Si. Meaning a negative result may not be truly negative, just an artifact of preparation. The more preparative steps, the more likely the elements can be lost. If they are bound to some molecules in the samples, and not labile, then you should be OK. But check this with a positive control!
Phil ------------- Philip Oshel Imaging Facility Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 989 774-3576 office
Have you tried VLC media player? It's a free, open-source media player that converts most video formats. I don't know if it works with TVIPS, but if not, the people who put out VLC can probably write a converter.
------------- Philip Oshel Imaging Facility Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 989 774-3576 office
Low vacuum may help with beam heating, but even at really low vacuum, like 500 Pa or higher, it's still a vacuum. Probably a better one than the vacuum in your vacuum-insulated coffee mug. So, I doubt it will help much.
You can do EDS analysis in low vacuum, just not mapping. There's a more-or-less big beam skirt (Dale Newbury has written good articles on this).
Phil ------------- Philip Oshel Imaging Facility Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 989 774-3576 office
X-from: Susan Van Horn {susan.vanhorn-at-stonybrook.edu}
Hello - saw you post - now this is going back more years than i will admit to - we did paraffin sections for SEM - what i remember is we cut paraffin sections pretty thick and placed them on a piece of glass - coverslip or slide that was scored and broke to the size of the SEM stub.....we had to critical point dry them which if i remember for biological material iwent through xylene at some point which got rid of the paraffin .....then glued it onto the SEM stub, coated it with gold palladium and viewed it in the SEM....not sure about the X-Ray analysis - we did not have that on the SEM hope this helps Sue
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Message: Dear colleagues, We would like to validate a method with SEM (meaning that another preparation method more suited to SEM cannot be considered) and this would require the analysis of paraffin blocks in SEM and I fear that this would melt the paraffin. Has any of you experience with observation of paraffin-embedded material in SEM? Optimally we would like to perform EDX analysis on the paraffin block surface, which would require enough kV to see Al and Si (I don't know the energy lines off the top of my head). Do you think that the electron beam could heat the paraffin up to the melting point ? Would it be possible to circumvent this by coating the sample with a "thick" layer of conductor? Best regards, Stephane
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*************************************************************** Susan C. Van Horn, M.A. C-MIC TEM Facility Office of Scientific Affairs Life Sciences Building Room 046 SUNY-at-Stony Brook Stony Brook, NY 11794-5200 phone: 631-632-8623 Â Â Â fax: 631-632-7728 email: susan.vanhorn-at-stonybrook.edu {mailto:susan.vanhorn-at-sunysb.edu} Central Microscopy Imaging Center (C-MIC) website: https://osa.stonybrookmedicine.edu/research-core-facilities/microscopy/services
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Email: luca.piazza-at-dectris.com
Name: Luca Piazza
Organization: Dectris LTD
Title-Subject: [Filtered] Product Engineer Electron Microscopy Position in DECTRIS
This is the perfect position for a young scientist with experience in electron microscopy who wants to transition from academia to industry. The candidate will work in close collaboration with product development, production, and business development, and contribute to shaping the future of electron microscopy detectors.
Thanks Luca
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Exciting changes are here for submissions to the Microscopy & Microanalysis 2020 meeting.
We know that submitting figures that could have been used later in a paper, and writing a 2-page submission, have been an issue for some.
NOW, there is the option of submitting 2000 characters (including spaces) with no figures. That corresponds to about 300 words. The maximum submission is 6000 characters and 2 figures.
Do note that for a student travel award 2 figures are required, for proper evaluation.
Students and post-docs who submit abstracts without the 2 required figures are still eligible for on-site poster awards!
AND M&M 2020 is excited to offer Childcare services for children ages 6 months - 12 years. Services will be available Monday through Thursday, August 3-6 for full days, half-days, or by the hour. Registration in advance is highly recommended and will be available after May 1, 2020.
The M&M 2020 Submission Site is now open!
Visit the M&M 2020 website: https://www.microscopy.org/MandM/2020/index.cfm
and click on the Submit Your Paper button
Submission deadline is Friday, February 21, 11:59 PM, U.S. Pacific Time.
Please note: MSA is using a new submission site and data collections vendor this year, so don't be alarmed if everything looks a little different!
Sincerely, Esther
--- Esther Bullitt, Ph.D. Associate Professor President 2020, Microscopy Society of America Dept. of Physiology & Biophysics Boston University School of Medicine 700 Albany Street, Room W302 Boston, MA 02118-2526
From geracore539shbda-at-gmail.com Sun Dec 15 07:01:34 2019 Return-Path: {geracore539shbda-at-gmail.com} Received: from gmail.com (a5.p8y9u4.cn [23.228.73.182] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id xBFD1XWt024104 for {microscopylistserverarchive7-at-microscopy.com} ; Sun, 15 Dec 2019 07:01:33 -0600 Message-ID: {439A2AF9.4CDEF390-at-gmail.com}
Dear All, We are pleased to announce the 2020 Porto AFM Training Workshop. This will take place in the beautiful city of Porto, Portugal, between the 6th to the 9th April. This will be the seventh edition of this course, and it’s always popular. Amongst other things, we cover AFM instruments, modes, data analysis, sample preparation, force spectroscopy, etc. As well as lectures the students have practical training on microscope use, image processing, and sample preparation. There is more info at afmhelp.com/course, and enquiries should go to afmhelp-at-gmail.com. You can also use this email address to reserve a spot on the course. We highly recommend that you book as early as possible, as places are very limited.
Peter Eaton
____________________________________________________________________________ Atomic Force Microscopy Dr Peter Eaton and Dr Paul West Available through all good bookshops, or direct from Oxford University Press at: http://ukcatalogue.oup.com/product/9780199570454.do http://afmhelp.com
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Ling Ling, I was planning to cut more this week, I will not be able to run your samples this week. I will be away from 12/21-Jan2. Sincerely, Michael Delannoy
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Title-Subject: [Filtered] Luxol-Fast Blue stain to plastic sections
Message: Dear List,
We are locating demyelination in white matter in CNS with a toxin administration. It has been well documented by Luxol-Fast Blue stain by using paraffin sections or cryo-stat sections. We were wondering if anyone is aware that Luxol-Fast Blue stain can be applicable to 1 micron thick plastic sections. If not, does anyone know a staining method that applicable to plastic sections to demonstrate lesion with demyelination? Any suggestions are appreciated. Thank you!
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Email: AFELL-at-2SPI.COM Name: Andrew Fell
Organization: Structure Probe
Title-Subject: [Filtered] TEM Film Issue
Message: I am working on a JEOL 1200-EX that still uses film for imaging. Recently, the film started to come out completely under-exposed. It was discovered that the film exposure setting had changed to 20 out of 20. Since then, we have tried many different parameters and have had no positive results as far as useful images. After adjusting the FSE we have an image again, but now we are struggling to obtain a scale of contrast. Despite being in-focus and displaying layers of contrast on screen - the film continues to develop in a more black-and-white situation.
We have gone through changing accelerating voltage, new film, new developing chem, exposure times, the full range of FSE, aperture adjustments and we are still hitting a wall. Has anyone dealt with this or (preferably) know how to resolve this issue? Thank you in advance!
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it might be of utmost interest which „kind of plastic“ = resin you use for embedding the white matter CNS tissue in your experimental setting.
There might be some techniques available, but also the use of these particular and special techniques might depend on the resin type you embed your specimens. What comes to my mind: Epoxide(s) like Epon, EMBED 812 and others Acrylates, Technovit’s, etc… Application of dyes with / without deplasticizing …. etc., etc.
Only as a quick „shot into the blue“ as examples to look for other possibilities: Toluidine-Blue: https://www.researchgate.net/publication/45648669_Wallerian-like_axonal_degeneration_in_the_optic_nerve_after_excitotoxic_retinal_insult_An_ultrastructural_study
You even might have a look into the article by the famous Prof. Dr. J. Kiernan, DOI: 10.1179/his.2007.30.2.87 Histochemistry of Staining Methods for Normal and Degenerating Myelin in the Central and Peripheral Nervous Systems Article (PDF Available) in Journal of histotechnology 30(2):87-106 · June 2007 
or: at: http://europepmc.org/article/PMC/3663462 Clin Neuropathol. 2012 Jan-Feb; 31(1): 7–23. Published online 2011 Dec 27. doi: 10.5414/NP300468 PMCID: PMC3663462 PMID: 22192700 WEIS et al. Processing of nerve biopsies: A practical guide for neuropathologists Further or more detailed proposals when we know about the resin you use…
Best wishes and regards, W.H.M,
======================================================= MUSS Wolfgang Dr. phil. [OR i. R.] RETIRED Ignaz-Rieder-Kai 19/6 A-5020 SALZBURG Ă–sterreich-AUSTRIA Mobile-Tel.: 0043(0)676 5 369 456 E-mail: wij.muss-at-aon.at E-Mail altern.: womuss-at-gmail.com
FRMS, Retired Member of MSA & other (Inter-)National Societies =======================================================
Von: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Gesendet: Donnerstag, 19. Dezember 2019 15:45 An: wij.muss-at-aon.at Betreff: [Microscopy] Luxol-Fast Blue stain to plastic sections ---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Dear List,
We are locating demyelination in white matter in CNS with a toxin administration. It has been well documented by Luxol-Fast Blue stain by using paraffin sections or cryo-stat sections. We were wondering if anyone is aware that Luxol-Fast Blue stain can be applicable to 1 micron thick plastic sections. If not, does anyone know a staining method that applicable to plastic sections to demonstrate lesion with demyelination? Any suggestions are appreciated. Thank you!
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Maybe there is still a PDF manual for this old hardware somewhere?
All the best,
Stefan
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----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
==============================Original Headers============================== 14, 23 -- From stefan.diller-at-t-online.de Thu Dec 19 13:09:31 2019 14, 23 -- Received: from mailout08.t-online.de (mailout08.t-online.de [194.25.134.20]) 14, 23 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id xBJJ9Vuc029590 14, 23 -- for {microscopy-at-microscopy.com} ; Thu, 19 Dec 2019 13:09:31 -0600 14, 23 -- Received: from fwd35.aul.t-online.de (fwd35.aul.t-online.de [172.20.27.145]) 14, 23 -- by mailout08.t-online.de (Postfix) with SMTP id 973A74112F98 14, 23 -- for {microscopy-at-microscopy.com} ; Thu, 19 Dec 2019 19:06:06 +0100 (CET) 14, 23 -- Received: from mac-pro.local (TDo6x0ZVwhv1V-vH4uNBbe-jmHGsZhugoOFC1Wa5+wuoOqC91DhkOQKPpq7wxByQzC-at-[31.16.250.216]) by fwd35.t-online.de 14, 23 -- with (TLSv1.2:ECDHE-RSA-AES256-GCM-SHA384 encrypted) 14, 23 -- esmtp id 1ii0BQ-1ZJ71k0; Thu, 19 Dec 2019 19:05:56 +0100 14, 23 -- To: "Microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 14, 23 -- From: Stefan Diller {stefan.diller-at-t-online.de} 14, 23 -- Subject: Deben Sprite Stagecontroller (serial #111) Manual needed 14, 23 -- Message-ID: {aabd5c56-612c-b07d-2015-9a2bcf0a9028-at-t-online.de} 14, 23 -- Date: Thu, 19 Dec 2019 19:05:55 +0100 14, 23 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:68.0) 14, 23 -- Gecko/20100101 Thunderbird/68.3.1 14, 23 -- MIME-Version: 1.0 14, 23 -- Content-Type: text/plain; charset=windows-1252; format=flowed 14, 23 -- Content-Transfer-Encoding: 7bit 14, 23 -- Content-Language: en-GB 14, 23 -- X-ID: TDo6x0ZVwhv1V-vH4uNBbe-jmHGsZhugoOFC1Wa5+wuoOqC91DhkOQKPpq7wxByQzC 14, 23 -- X-TOI-MSGID: 98d00c7b-b46a-45d0-a42f-cd5f203aa600 ==============================End of - Headers==============================
From golasage265y-at-gmail.com Fri Dec 20 00:35:15 2019 Return-Path: {golasage265y-at-gmail.com} Received: from gmail.com (a3.isbfbf.cn [23.228.73.189] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id xBK6ZEvN018724 for {microscopylistserverarchive7-at-microscopy.com} ; Fri, 20 Dec 2019 00:35:15 -0600 Message-ID: {DED5B935.4B494930-at-gmail.com}
X-from: unocicrr-at-ornl.gov
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Email: unocicrr-at-ornl.gov Name: Raymond Unocic
Organization: Oak Ridge National Laboratory
Title-Subject: [Filtered] Postdoctoral Position at ORNL on in situ directed transformation of materials
Message: Postdoc Position available in the Center for Nanophase Materials Science at Oak Ridge National Laboratory
Overview: The Center for Nanophase Materials Sciences (CNMS) is seeking a Postdoctoral Research Associate to support the development and application of methods for directed manipulation of materials using electron and ion beam instrumentation. The specific focus will be to develop methods for controlling and understanding electron/ion beam interactions to permit a mechanistic understanding of competing factors that will guide the directed fabrication and atomic manipulation of 1D, 2D and 3D nanostructure architectures. As a Postdoctoral Research Associate, you will contribute to the development of novel scan control and feedback strategies, perform in situ experiments under controlled environmental conditions and as a function of external stimuli and work closely with ORNL scientists on materials characterization, synthesis and theory in order to develop experimentally validated predictive models. You will also support the development of a computer aided design capability for the accurate and precise deposition of complex nanoscale mesh object models. Multi–functional deposition will be explored using multiple precursor gases and/or multiple ion sources to tailor 3D shapes and selectively incorporate function through composition control. You will also collaborate with artificial intelligence/machine learning experts at ORNL to guide material transformations.
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==============================Original Headers============================== 10, 54 -- From microscopy.listserver-at-gmail.com Fri Dec 20 08:26:36 2019 10, 54 -- Received: from mail-io1-f53.google.com (mail-io1-f53.google.com [209.85.166.53]) 10, 54 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id xBKEQa2a028154 10, 54 -- for {microscopy-at-microscopy.com} ; Fri, 20 Dec 2019 08:26:36 -0600 10, 54 -- Received: by mail-io1-f53.google.com with SMTP id n21so7785524ioo.10 10, 54 -- for {microscopy-at-microscopy.com} ; Fri, 20 Dec 2019 05:23:14 -0800 (PST) 10, 54 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 10, 54 -- d=gmail.com; s=20161025; 10, 54 -- h=subject:references:to:from:message-id:date:user-agent:mime-version 10, 54 -- :in-reply-to:content-language:content-transfer-encoding; 10, 54 -- bh=5zvBQ1vxbEerMaD/rQJz1ljZpHXebws8VDK1Tl+Mec8=; 10, 54 -- b=ajTDZbKVNgllG4fzj9yeDR40GiQghs4jQCRVvUfXAW+EO995aiv43kb6p66Z12akjq 10, 54 -- ZFSHDlXrofAHxyLDThNa8nh5RGzHW8GfV5glYEB367us7hvpiS0J3rB/hGaLPy/X/JJS 10, 54 -- 4CCerd0CbVMow+PGHdrejnDgBOITcjX7azIQPNLBkivgY8LvMj69onhdWoM03r+RN/l/ 10, 54 -- ncE8ruGJ8DkmtPyl2VJKht7Wo/59Tm9bHTxTnWUXofOmQ+1aK242vOiEBmSCAz/ODU3R 10, 54 -- luQ3sgotmrxo0mKiQpQMx2z+VlAQ5d4U4y0wA/dIhIZ2ZIPI5jshlAbzeSXug3++Qo5L 10, 54 -- MGyA== 10, 54 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 10, 54 -- d=1e100.net; s=20161025; 10, 54 -- h=x-gm-message-state:subject:references:to:from:message-id:date 10, 54 -- :user-agent:mime-version:in-reply-to:content-language 10, 54 -- :content-transfer-encoding; 10, 54 -- bh=5zvBQ1vxbEerMaD/rQJz1ljZpHXebws8VDK1Tl+Mec8=; 10, 54 -- b=dYI19P1J+D3FF2UAd0uaer1l21H3oFtRBzMO8Peq3AjTXVq/VzVgPnTz3QG/0AyunG 10, 54 -- oRUDw8uFha7aRDX4N+DhxeQXUNiM022MzkJNXm3q1dvfYaHirUNCHJx2Hjk8lQYWo/Iv 10, 54 -- EZG7eg9aEOATV3LI0drho0lKHauQP3J12JPF/hqk9bRizL5g61NZqAa+e+wUEmUty3Sj 10, 54 -- tJ3qPVKlx8bj8hK8yfRbk2rcdhEu5/tbFS17Qtyu/OFOZ0YIML2bcIQ29+fMPcjuQVM/ 10, 54 -- qEH4VbHay+jaSvdh5dJHsfdIjOtQJAvREquDKVqvsoIQhVrNrvFvuF2tSH/Ct+67JX1B 10, 54 -- v3MQ== 10, 54 -- X-Gm-Message-State: APjAAAVc/IpnqgacecHW1rb/45esaaCIuPz2YOIOfhRZTgscgPZ4QOZf 10, 54 -- UcaYy6y25BCg0tQ8iwIWrHrY7mQg 10, 54 -- X-Google-Smtp-Source: APXvYqyD3m/OhnbUd5QjQhuQOzi9aAsZyC9n/GyekJPuppPDaO8FCdL50e+/4/n93uPWt1N7+XI8qA== 10, 54 -- X-Received: by 2002:a02:a309:: with SMTP id q9mr12073820jai.141.1576848194361; 10, 54 -- Fri, 20 Dec 2019 05:23:14 -0800 (PST) 10, 54 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:b015:193b:6f94:8a2d]) 10, 54 -- by smtp.googlemail.com with ESMTPSA id m27sm4578297ilb.53.2019.12.20.05.23.13 10, 54 -- for {microscopy-at-microscopy.com} 10, 54 -- (version=TLS1_3 cipher=TLS_AES_128_GCM_SHA256 bits=128/128); 10, 54 -- Fri, 20 Dec 2019 05:23:13 -0800 (PST) 10, 54 -- Subject: viaWWW:Postdoctoral Position at ORNL on in situ directed 10, 54 -- transformation of materials 10, 54 -- References: {201912191706.xBJH6GgH019436-at-microscopy.com} 10, 54 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 10, 54 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} 10, 54 -- X-Forwarded-Message-Id: {201912191706.xBJH6GgH019436-at-microscopy.com} 10, 54 -- Message-ID: {6845373a-7757-c450-b8f7-ebbc60c84277-at-gmail.com} 10, 54 -- Date: Fri, 20 Dec 2019 07:23:12 -0600 10, 54 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.14; rv:68.0) 10, 54 -- Gecko/20100101 Thunderbird/68.3.1 10, 54 -- MIME-Version: 1.0 10, 54 -- In-Reply-To: {201912191706.xBJH6GgH019436-at-microscopy.com} 10, 54 -- Content-Type: text/plain; charset=windows-1252; format=flowed 10, 54 -- Content-Language: en-US 10, 54 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
Hi, can anyone share the experience with the RMC microtome? In my area (Ireland), The RMC device is ca. 20% cheaper than Leica. Are there any hidden disadvantages in it? Thanks, Dimitri
/Dimitri Scholz, PhD, Dr. Sci. Director of Biological Imaging Conway Institute University College Dublin UCD Belfield, Dublin 4 Ireland Office: +353-1 716 6736 Mobile: +353-87-7961547 Web: http://conway.ucd.ie/coretech/ LinkedIn Profile {https://www.linkedin.com/profile/view?id=AAIAAAVjAg0BCmFc5Rk54thvrW6k0ZirNtCW_qo&trk=nav_responsive_tab_profile_pic} Publications {https://www.researchgate.net/profile/Dimitri_Scholz/publications}
Electronic mail to, from, or within the University may be the subject of a request under the Freedom of Information Acts 1997 and 2003/
X-from: Francesco Pasqualini {francesco.s.pasqualini-at-gmail.com}
Hi, I will start my ERC-funded lab in Italy in 2020, which means I am fortunate to be shopping for a confocal instrument right about now.
Since I do mostly live experiments (more details below) I was going to get a spinning disk system. But, I realized that the new scanning confocals are also relatively fast and gentle. The question is how much (if at all) slower and harsher are they with respect to spinning disks?
More details: - I do a lot of live experiments (traction force microscopy and voltage/calcium-sensitive dyes) on engineered cells (300x300 um FOV) and tissues (600x600 um FOV) in 2D or 3D ( {300 um thick) that I complement with immunostainings after fixation. Needed acquisition rates go from {1 fps to 100s depending on the application.
- Originally, I was oriented towards a spinning disk confocal (Yokogawa/SORA, Crest) and was looking at ways to deal with the issues of confocality on the 3D tissue (600x600x300 um volumes) case. But, I realized all vendors have new point scanning confocal (980+Airyscan2, FV3000, A1R) with resonant scanners that acquire full frames/ROIs at 10s/100s of fps
- Of course, structured illumination (Elyra-7, N-SIM) and light-sheet microscopes (QuVi) are also appealing but relatively untested in my applications of interests....
The application specialists from all vendors in my area have been great to work with but since my lab is not up and running, yet, I can't demo these systems directly. Therefore, I could use help and feedback, especially from people who have had related experiences in the recent past.
Thanks, Francesco
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From klostocc091pyje-at-gmail.com Tue Dec 24 02:46:51 2019 Return-Path: {klostocc091pyje-at-gmail.com} Received: from gmail.com (a3.68474.cn [23.228.73.183] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id xBO8koY4010376 for {microscopylistserverarchive7-at-microscopy.com} ; Tue, 24 Dec 2019 02:46:51 -0600 Message-ID: {7085ACE4.485D3B37-at-gmail.com}
X-from: kevran44-at-aol.com
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Email: kevran44-at-aol.com Name: Dan Connors
Organization: EDS Services Inc
Title-Subject: [Filtered] High Tension Cable
Message: I am looking for a company that can repair Electron Microscopes High-Tension Cables. Is there a company I can send the gun and old cable to and have a new cable installed?
Thanks Dan
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We have both types of ultramicrotomes in our lab and both work well. I cannot think of any reason (other than the obvious) on why you would choose one over the other. That being said.... one is located in Arizona and I’ve always had excellent and rapid solutions to issues with Boekeler RMC.
John Shields
} On Dec 20, 2019, at 7:33 AM, microscopy.listserver-at-gmail.com wrote: } }  } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: Dimitri Scholz {dimitri.scholz-at-ucd.ie} } } Hi, can anyone share the experience with the RMC microtome? In my area (Ireland), The RMC device is } ca. 20% cheaper than Leica. Are there any hidden disadvantages in it? } Thanks, } Dimitri } } } /Dimitri Scholz, PhD, Dr. Sci. } Director of Biological Imaging } Conway Institute } University College Dublin UCD } Belfield, Dublin 4 } Ireland } Office: +353-1 716 6736 } Mobile: +353-87-7961547 } Web: http://conway.ucd.ie/coretech/ } LinkedIn Profile } {https://www.linkedin.com/profile/view?id=AAIAAAVjAg0BCmFc5Rk54thvrW6k0ZirNtCW_qo&trk=nav_responsive_tab_profile_pic} } Publications {https://www.researchgate.net/profile/Dimitri_Scholz/publications} } } Electronic mail to, from, or within the University may be the subject of a request under the Freedom } of Information Acts 1997 and 2003/ } } ==============================Original Headers============================== } 5, 53 -- From microscopy.listserver-at-gmail.com Fri Dec 20 08:33:38 2019 } 5, 53 -- Received: from mail-io1-f45.google.com (mail-io1-f45.google.com [209.85.166.45]) } 5, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id xBKEXcWZ002569 } 5, 53 -- for {microscopy-at-microscopy.com} ; Fri, 20 Dec 2019 08:33:38 -0600 } 5, 53 -- Received: by mail-io1-f45.google.com with SMTP id v18so9399511iol.2 } 5, 53 -- for {microscopy-at-microscopy.com} ; Fri, 20 Dec 2019 05:30:17 -0800 (PST) } 5, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 5, 53 -- d=gmail.com; s=20161025; } 5, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 5, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 5, 53 -- bh=lwFHraSzZeYLB1q0Lv8/fQBxCunRSqgmZ/ZPeyv6GU4=; } 5, 53 -- b=TcuHTfWaNp9gvh5BQQa/wdCHRdDdJSxB+9U7hYiEPQDMygHaMAy5XefkeC8NJSTJvO } 5, 53 -- VDxAFNmKi4+jdDPDGfxJLZpysr2Fu8hRJGH8LqJqYaMWyUedmCF+1io9e6PqwPpK29HK } 5, 53 -- 8KPDP7B2mfwYZd9uW++gK1i9Xsd9Wa3cm3SO5U4JLcsMYpgudSkO0B3RcKiINvizhTu8 } 5, 53 -- iDPh26CtweW+6aASbC5pt+xoc7YsniZx7tmsuC+XX631PftbLhGX4d9u3rSn4eJu3jOv } 5, 53 -- lNcaJ15CLlGIbxKxOyrEaeQnGQk4pvPZzHT6U0MEVnBQLSThRhuxm/MC0WzdAqDSG4dW } 5, 53 -- XNfQ== } 5, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 5, 53 -- d=1e100.net; s=20161025; } 5, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 5, 53 -- :user-agent:mime-version:in-reply-to:content-language } 5, 53 -- :content-transfer-encoding; } 5, 53 -- bh=lwFHraSzZeYLB1q0Lv8/fQBxCunRSqgmZ/ZPeyv6GU4=; } 5, 53 -- b=i94zsC/LB/EnAhAnPDHH+zhFauam4f2ReT7F6Rto5mofYEUnrC16OAI5J38bgrFU60 } 5, 53 -- SnlvErSiAxZ0AbJKKzD8CymdOkG7ucKY4z6Y7u0aB3fol984SHrf7p9HBTTKsxM67k7q } 5, 53 -- ZO0uR1IhMl8ZzWXSQsxGa+N+A6/xeldG3wRV4Y1J+5LPUAs8gacbQxyvjTNdfxsoEskP } 5, 53 -- e2W4c7oTCOo23J1Q5JazddMRzCVxoa8OgBDhvWsopm33fro4v9zPOpMR8JOmwYBJvJ4S } 5, 53 -- Px9DEaoY8wpEUUDo1rHU9TEn2dvw4Sv9YJmwYwm24Jeo+B5mCFNbbtK0OGyDI5ays7Fp } 5, 53 -- Lihw== } 5, 53 -- X-Gm-Message-State: APjAAAXfRvFs+r39C0noElb6YSTzsJG8t9+Yuk45WTl0ZTzHhwWKAmD0 } 5, 53 -- jBnyYuLAsZV5HNpzKpFO/nBhMbPF } 5, 53 -- X-Google-Smtp-Source: APXvYqz9zSKde74pG3DdBE8O84AsitsFohWaPLbLhwk7OjcoCxWGLW1uaIXDvzG3s71TBM4vBSkEGQ== } 5, 53 -- X-Received: by 2002:a5d:93d4:: with SMTP id j20mr9011105ioo.68.1576848617039; } 5, 53 -- Fri, 20 Dec 2019 05:30:17 -0800 (PST) } 5, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:b015:193b:6f94:8a2d]) } 5, 53 -- by smtp.googlemail.com with ESMTPSA id f76sm4585933ild.82.2019.12.20.05.30.16 } 5, 53 -- for {microscopy-at-microscopy.com} } 5, 53 -- (version=TLS1_3 cipher=TLS_AES_128_GCM_SHA256 bits=128/128); } 5, 53 -- Fri, 20 Dec 2019 05:30:16 -0800 (PST) } 5, 53 -- Subject: Fwd: RMC ultramicrotome } 5, 53 -- References: {CACXP4qsxkHdyM0E5HMRV6T57pgZXZS1x8nTO+J4VgvMb-Ybrgw-at-mail.gmail.com} } 5, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 5, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 5, 53 -- X-Forwarded-Message-Id: {CACXP4qsxkHdyM0E5HMRV6T57pgZXZS1x8nTO+J4VgvMb-Ybrgw-at-mail.gmail.com} } 5, 53 -- Message-ID: {bf44573b-759a-c6c1-9931-45ca3fb2a3ac-at-gmail.com} } 5, 53 -- Date: Fri, 20 Dec 2019 07:30:16 -0600 } 5, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.14; rv:68.0) } 5, 53 -- Gecko/20100101 Thunderbird/68.3.1 } 5, 53 -- MIME-Version: 1.0 } 5, 53 -- In-Reply-To: {CACXP4qsxkHdyM0E5HMRV6T57pgZXZS1x8nTO+J4VgvMb-Ybrgw-at-mail.gmail.com} } 5, 53 -- Content-Type: text/plain; charset=utf-8; format=flowed } 5, 53 -- Content-Language: en-US } 5, 53 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers==============================
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