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Happy New Year Colleagues;
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jpshield-at-uga.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: jpshield-at-uga.edu Name: John P Shields
Organization: University of Georgia
Title-Subject: [Filtered] Biological TEM Workshop
Message: This intensive, three-day workshop will provide a practical and basic theoretical introduction to the Transmission Electron Microscope and biological sample preparation techniques. Each day will consist of lecture, discussion and hands-on training led by GEM staff. Who: Anyone requiring training on TEM and biological sample preparation. The workshop will be limited to 6 participants based on the availability of equipment. When: Monday through Wednesday, March 14-16, 2018, 8am-5pm each day (lunch is provided)
Where: 154 Barrow Hall, University of Georgia, Athens, GA 30602
Registration: Contact John Shields (jpshield-at-uga.edu) for more information and to sign up. Registration requires iLab account through the GEM website. https://uga.ilabsolutions.com/account/login
Deadline: March 5, 2018 ***************** Past participants include Merck & Co., Connecticut State, Breathitt Vet Center, Univ. of Chicago, Stowers Inst. for Medical Research, West Virginia State
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ben-at-protochips.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: ben-at-protochips.com Name: Benjamin Jacobs
Organization: Protochips, Inc
Title-Subject: [Filtered] Applications Scientist In Situ TEM - immedate opening at Protochips
Message: Protochips has an immediate opening for a marketing applications scientist. Candidates must have a strong background in TEM, and experience with in situ TEM such as heating, gas, liquid or mechanical, is highly preferred. Travel up to 40%, mostly domestic, but some international to Europe and East Asia. Candidates must also be a US citizen or already be authorized to work in the US. Position is located in the Raleigh/Durham area in North Carolina, and candidate must be willing to relocate.
Please click the following link for more information:
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both walbrid-at-cshl.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Hiring - Research Associate - Electron Microscopy Technologist Position
Message: Research Associate Electron Microscopy Technologist
Cold Spring Harbor Laboratory seeks a highly motivated dedicated individual to work in a state-of-the-art Microscopy Shared Resource.
The individual must have extensive practical expertise in biological sample preparation for transmission and scanning electron microscopy of animal tissues and mammalian cell lines. Hands-on knowledge of confocal and widefield fluorescence microscopy would also be a plus.
The candidate will help users design innovative experiments and they will carry out sample preparation and imaging as well as assist in data interpretation.
Excellent verbal and written communication skills, ability to work with multiple users in a supporting role, and ability to work independently and proactively with limited supervision are essential. A Bachelors degree in biology or related discipline is required. One to three years of experience working in a Microscopy Shared Resource is preferred.
How to Apply
Interested individuals should apply for this position via the CSHL careers website at http://cshl.peopleadmin.com/postings/11688
Position Number 01779-R
Applicants should include a resume along with a description of their practical expertise and the names as well as email addresses of 3 references.
Cold Spring Harbor Laboratory is a world-renowned research and educational institution recognized internationally for its excellence in ground-breaking research programs in cancer, neuroscience, plant biology, genomics, and bioinformatics and broad educational mission.
For more information about CSHL, please visit us at www.cshl.edu
CSHL is an EO/AA Employer. All qualified applicants will receive consideration for employment and will not be discriminated against on the basis of race, color, religion, sex, sexual orientation, gender identity, national origin, age, disability or protected veteran status.
VEVRAA Federal Contractor
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both SLC6-at-Lehigh.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: SLC6-at-Lehigh.edu Name: Sharon Coe
Organization: Lehigh University
Title-Subject: [Filtered] Lehigh University Microscopy School 2018
Message: Now accepting registrations for the 48th Lehigh Microscopy School which will be held on the campus of Lehigh University, Bethlehem, PA, June 3-8, 2018. All courses, lecturers, and instrumentation will be together for what promises to be a phenomenal week! Course offerings include: SEM and X-ray Microanalysis Introduction to SEM and EDS for the New Operator Focused Ion Beam Instrumentation and Applications Problem Solving: Interpretation and Analysis of SEM/EDS/EBSD Data Quantitative X-ray Micoanalysis Problem Solving Using EDS and WDS Techniques Scanning Transmission Electron Microscopy: From Fundamentals to Advanced Applications. Register and pay in full by April 13 for an early bird discount! Contact: Sharon Coe (sharon.coe-at-Lehigh.edu or 610-758-5133). See www.Lehigh.edu/microscopy for registration form, prices, and details about courses, lecturers, and instrument suppliers. Scholarships available for full-time graduate students.
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X-from: Guobin Hu {gb_hu-at-yahoo.com} To: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com}
Hi microscopy subscribers,
We recently purchased an EMS T150 ES for carbon coating. Originally we had a standard chamber. The coating was very inconsistent. We contact EMS and EMS sent us an extended height chamber and advised us to control the coating by monitoring the FTM (Film Thickness Monitor) reading instead of by editable profiles. It does make the coating more controllable. However, the results are still not consistent. For example, I coated carbon of 9 nm by FTM and also carbon of 12.5 nm by FTM. The 9 nm thick carbon actually looked thicker than 12.5 nm. I would appreciate it if users here can share your experience with me how to coat carbon with consistent and accurate thickness. On the other hand, I would also appreciate it if you can make recommendations on carbon coaters.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both driscollg2-at-winthrop.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Need Fiji script to subtract background noise
Message: Due to the large number of images and insufficient amount of ram, I am unable to load both image sequences (~1900 per) on Fiji in order to use the standard "image calculator" to subtract my images from the RFP (A) channel from GFP (B) channel to reduce background noise. I am looking for a script that would allow me to subtract Image 1A - Image 1B = Image 1C, Image 2A - Image 2B = 2C... etc. Ultimately, I am looking to create a Z-stack from my subtracted images to reduce background noise to further analyze data. Login Host: 206.74.212.240 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
Check cooling water requirements of the SEM (flow, dissipated power) and Google for compatible chiller - there are literally hundreds if not thousands outlets selling them online, starting with amazon.com and zoro.com and ending with ebay.com and aliexpress.com; chiller for running SEM could be found with price tags below US$1,000. Valery
Valery Ray ============================== PBS&T, MEO Engineering Company 290 Broadway, Suite 298 Methuen, MA 01844, USA Phone: +1-978-305-0479 - leave a message Mobie: +1-978-305-0479 - leave a message E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
---------------------------------------------------------------------------------------------------- *From:* "microscopy.listserver-at-gmail.com" {microscopy.listserver-at-gmail.com} *To:* vray-at-partbeamsystech.com *Sent:* Thursday, January 11, 2018 9:49 AM *Subject:* [Microscopy] viaWWW: Water chiller for Hitachi SEM.
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-------- Forwarded Message --------
X-from: McClintock, Joel {jmcclin-at-uky.edu}
Ravi,
This only needs a flow of 1-1.5 liters/minute. Not really very much. Short term, tap water supply works fine. Risk of condensation and corrosion over time. Constantly running water, which no one likes as well.
What does flow sensor in back of misroscope indicate is the flow? YOu can change the sensor point on that flow gauge.
Pretty sure all you are cooling is the Diffusion pump. Think 700 Watt heater. I know the Neslab CFT33 is sufficient. Specs for it are "950 Watts at 20C 3240 BTU/hr at 20C 817 Kcal/hr at 20C". The CFT25 model may be not sufficient. The oldHaskris model RO33 will also work. You can find it's specs. Think mostany chiller with similar specs will work.
Joel
---------------------------------------------------------------------------------------------------- *From:* microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} *Sent:* Thursday, January 11, 2018 10:56:19 AM *To:* McClintock, Joel *Subject:* [Microscopy] viaWWW: Water chiller for Hitachi SEM.
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-------- Forwarded Message --------
X-from: Crane, Dan - OSHA {Crane.Dan-at-dol.gov}
I have a Hitachi S3500-N and have used a Haskris R-033 water cooled chiller for it. It was the recommended chiller by Hitachi. While possibly not the least expensive alternative, it has worked well for us. You can always call Hitachi High Technologies America, Inc. service department and see what they recommend.
We have used a variety of different chillers for our other microscopes and instruments, TEM, ICP, and ICP-MS. Each has to be matched to the power load of the instrument so that they are adequate and are not overworked so that they fail too soon.
The trick to know with SEM or TEM especially is that the chiller has to provide a very constant temperature with no pulsation. Temperature is important for magnification stability and absence of pulsation is essential for imaging and resolution. Not every chiller can provide either or both. You may not have seen any problems with city water for pulsation or for rapid temperature variance because of the nature of the source. However, when you go to a closed loop chiller, such problems can surface and be problematic, really degrading images.
You will need to look at the installation section of the S3500-N instrument and it will give the temperature and flow requirements for the tool. You can then go to Haskris, or any of the other suppliers and see what they can do.
Be very careful of used chillers. Pumps and motor-pump interfaces have a lot of wear, might start to pulse, and might simply give out with no warning. (Been there, done that.) Also, I just had a compressor develop what can only be called a fistula between the cooling water and the Freon lines. (We have relatively corrosive tap water.) This was an age-related failure.
I hope that this helps. Dan Crane
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Thursday, January 11, 2018 9:13 AM To: Crane, Dan - OSHA
-------- Forwarded Message --------
X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu}
Ravi,
A Haskris chiller will work well - you can get these in air-cooled versions. As for used … I don’t know. But it’s worth checking your university’s HVAC people. If they don’t have one hanging around, there might be one available surplus.
Phil ------------- Philip Oshel Imaging Facility Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 989 774-3576 office 989 774-7567 lab
Email: ravi.thakkar369-at-gmail.com Name: Ravi
Organization: Kansas State University
Title-Subject: [Filtered] Water chiller for Hitachi SEM.
Message: We have Hitachi S3500-N SEM. A chilled water from building supply was used to run the machine, but now pressure got dropped and we are not getting sufficient flow rate to keep machine running.I don't see chances of water pressure get increased. 1. Could you guys please suggest what could be the probable suggestion ? 2. Any suggestion, which recirculating chiller will work for Hitachi S3500-N SEM? 3. If you guys can suggest any source from where we can get used chiller as our budget is limited. Note : We do not have service contract. Login Host: 129.130.145.90
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both randy-nessler-at-uiowa.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: randy-nessler-at-uiowa.edu Name: Randy Nessler
Message: I'm wondering if there is a source for the following items used in our Balzers BAF 301 freeze fracture apparatus: Bal-Tec BU 020 023-T tungsten filaments Platinum pellets BD481505 (Balzers part number) Hollow end carbon rods to hold Pt pellets BD484055 (Balzers part number) Balzers Union BD 484 058 carbon rods
Those part numbers are off some I have on hand, but I hope to add to my inventory. Searching the internet, I saw the platinum inserts listed as BU 006 103-T, carbon rod for PT/C evaporation as BU 006 101-T and the carbon rods as BU 006 115. Another listing was "carbon set" BU 020 077-T, and Pt-C set BU 020 075-T. Unfortunately, these were citations in a publication rather than vendor inventory. Thanks for any help in advance, Randy
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ben-at-protochips.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: ben-at-protochips.com Name: Benjamin Jacobs
Organization: Protochips, Inc
Title-Subject: [Filtered] Seeking Applications Scientists for In Situ TEM in China
Message: Protochips has immediate openings for a applications scientists located in China. Candidates must have a strong background in TEM, and experience with in situ TEM such as heating, gas, liquid or mechanical, is highly preferred. Travel up to 50%, mostly within China, but some international to Europe and the United States for training and conferences.
Please click the following link for more information:
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both unocicka-at-ornl.gov as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: unocicka-at-ornl.gov Name: Kinga Unocic
Organization: Oak Ridge National Laboratory
Title-Subject: [Filtered] Postdoctoral Position at ORNL on advanced and in situ STEM
Message: There is a postdoctoral position open at ORNL in the Materials Science and Technology Division on Advanced and in situ STEM (nanomechanics and catalysts)
Qualified candidates can apply through the following link: https://recruiting.ornl.gov/sap/bc/webdynpro/sap/zornl_hrrcf_c_post_doc?sap-language=EN&sap-client=010#
then search for the "Postdoctoral Research Associate in Advanced STEM Characterization / NB50650181" Posting
Purpose We are seeking a Postdoctoral Research Associate to focus on the mechanical behavior of materials at the nanoscale level, investigate the morphological changes of catalysts during their life-cycle, and work with a team to develop advanced electron microscopy analysis methods. While this position is based in our Electron Microscopy (EM) group, you'll collaborate with a diverse group of experimentalists, theorists, and data scientists across different organizations within Oak Ridge National Laboratory (ORNL). This position resides within the Materials Science and Technology Division (MSTD), Physical Sciences Directorate at ORNL.
OVERVIEW: As a postdoc, you will support the two projects indicated above while advancing data acquisition and data analysis methods for electron microscopy. The mechanical research will be directed toward the correlation of composition and crystallographic orientation with mechanical response at the nanoscale level using in situ nano-compression and scanning transmission electron microscopy (STEM). The catalytic research will focus on analyzing structural changes from the atomic to mesoscale within catalysts and correlating structure, porosity, and compositional changes with applied synthesis and other applied treatments/reactions. To do this, youll use aberration-corrected scanning transmission electron microscopy (STEM) and imaging and analytical microscopy techniques, such as electron energy loss spectroscopy (EELS) and energy X-ray dispersive spectroscopy (EDS). You'll also work with a multidisciplinary research team to develop electron microscopy characterization techniques suitable for the catalyst and quantitative data analytic methods to determine the factors that govern catalytic processes at the atomic level.
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this is what I found: ok: page document from 2009.... not knowing whether Stacey Kirsch has ready or in stock your requested consumables https://www.emsdiasum.com/microscopy/products/brochures/2009/ems2_09.pdf p.8.: Our facility is equipped to handle the following Manufacturers: } } Balzers/Baltech email: sgkcck-at-aol.com or stacie-at-ems-secure.com
or: http://www.highland-scientific.com/balzersbn.html [NB: ok, This page last updated at 11.13 on Thursday, 14 November 2013] Contact details: Highland Scientific Unit 20, Bedford Business Centre Mile Road Bedford MK42 9TW England Tel. + 44 (0)1 234 216 636 Fax. + 44 (0)1 234 271 991 Sales-at-Highland-Scientific.com
As of 25.02.2008: LEICA Microsystems buys / bought BAL-TEC in Liechtenstein (sorry only in German) http://www.chemie.de/news/78525/erneuter-firmenerwerb-durch-leica-microsyste ms-bal-tec-in-liechtenstein.html so it might be at least possible to find the rep-site of LEICA Microsystems IOWA or USA and ask them about...
Last but not least (I was able some years ago also to help another US-colleague to order parts and consumables for former BALZERS - then BAL-TEC products now BALTIC PRAEparation.e.K.: Cf: http://www.baltic-praeparation.de/ (unfortunately I found only a German webpage... Claudia Kster Baltic Prparation e.K. Koppelheck 34b D-24395 Niesgrau Telefon: 0 46 43 / 18 65 43 Telefax: 0 46 43 / 18 65 54 Mobil: 0172 / 626 44 55 Email: baltic.praeparation-at-t-online.de
I've written to them using their the php form and asked a preliminary question for you...and I am sure they will answer your request written in English...
Best wishes to you and yours, Wolfgang
======================================================= MUSS Wolfgang Dr. phil. (PhD) [OR i. R. / en retraite / retired] Ignaz-Rieder-Kai 19/6 A-5020 SALZBURG sterreich-AUSTRIA Mobile-Tel.: 0043(0)676 5 369 456 E-mail: wij.muss-at-aon.at E-Mail altern.: womuss-at-gmail.com
FRMS, Retired Member of MSA
Scientific Profile at ResearchGate: http://www.researchgate.net/profile/Wolfgang_MUSS Former Head of Electron Microscopy Lab at Institute of Pathology SALK-LKH / Salzburger Landeskliniken | General Hospital and PMU (private) PARACELSUS MEDICAL UNIVERSITY of SALZBURG
Former Secretary and (until June2017) Board Member of the
SCUR {The Society for Cutaneous Ultrastructure Research} The Skin Imaging Society { www.scur.org } ====================================================================
Von: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Gesendet: Freitag, 12. Januar 2018 02:03 An: wij.muss-at-aon.at Betreff: [Microscopy] Balzers BAF 301 freeze fracture consumables source --------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
please copy both randy-nessler-at-uiowa.edu as well as the Microscopy Listserver --------------------------------------------------------------------------- Email: randy-nessler-at-uiowa.edu Name: Randy Nessler Organization: University of Iowa Title-Subject: Balzers BAF 301 freeze fracture consumables source Message:
I'm wondering if there is a source for the following items used in our Balzers BAF 301 freeze fracture apparatus: Bal-Tec BU 020 023-T tungsten filaments Platinum pellets BD481505 (Balzers part number) Hollow end carbon rods to hold Pt pellets BD484055 (Balzers part number) Balzers Union BD 484 058 carbon rods
Those part numbers are off some I have on hand, but I hope to add to my inventory. Searching the internet, I saw the platinum inserts listed as BU 006 103-T, carbon rod for PT/C evaporation as BU 006 101-T and the carbon rods as BU 006 115. Another listing was "carbon set" BU 020 077-T, and Pt-C set BU 020 075-T. Unfortunately, these were citations in a publication rather than vendor inventory. Thanks for any help in advance, Randy
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Email: jdykes-at-wvc.edu Name: Jeff Dykes
Organization: Wenatchee Valley College
Title-Subject: [Filtered] Help with ASPEX 3025 SEM
Message: Hello All,
My department just received a Scanning Electron Microscope and we are looking for some assistance. We are in need of:
-An operators manual that could be copied and sent to us. It is for a ASPEX 3025 SEM. -Guidance or protocols to prepare (fix) samples such as plants, bacteria, insects. -Any other documents, tips, etc.
Thank you,
Jeff Dykes Science Faculty Wenatchee Valley College 116 West Apple Omak, WA 98841 (USA)
jdykes-at-wvc.edu 509 422-7876
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Email: bassimn-at-mcmaster.ca Name: Nabil Bassim
Organization: McMaster University
Title-Subject: [Filtered] Postdoc - Advanced TEM of Steel/Thin Films
Message: Postdoc position in Advanced Microscopy of Steel Microstructure and Thin Films - Position Available Now.
A postdoctoral position is available in the performance of two distinct projects. The researcher will use the state-of-the-art aberration-corrected TEM's at the Canadian Centre for Electron Microscopy (ccem.mcmaster.ca) located at McMaster University, Hamilton Canada. The CCEM is a world-class imaging facility and is equipped with 4 TEM's, including two aberration-corrected microscopes (FEI Titans). The first project is to study precipitation mechanisms of Nb-rich steels after hot rolling. This work requires knowledge of chemical imaging, diffraction methods, and an understanding of strain measurement. The second project is the study of the thin film growth process, from 2-D materials up to ALD-grown films. This requires high-resolution imaging (TEM and STEM), EELS, and EDS.
The postdoc will be part of the Bassim research group, with a strong collaboration with Prof. Zurob's research group.
Applicants should have a strong background in electron microscopy, preferably with aberration-corrected (S)TEM experience. Additional background in metallurgy or thin films is a bonus. Ability to work in a team, mentor graduate students, as well as excellent verbal and written communication skills are expected.
McMaster University is located in Hamilton, Ontario, within a 45 minute drive from downtown Toronto, which a world class city and 45 minute drive to Niagara Falls.
To apply, please send a cover letter, curriculum vitae including references, and 1-2 relevant published papers to the e-mail below.
Nabil Bassim Associate Professor Department of Materials Science and Engineering, JHE 258 McMaster University 1280 Main Street West Hamilton, ON L8S 4L8 CANADA nbassim.mcmaster.ca bassimn-at-mcmaster.ca
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Email: paul.carpenter.epma-at-gmail.com Name: Paul Carpenter
Organization: Washington University
Title-Subject: [Filtered] Solid State X-ray Spectrometry at 50 Years: Session A11 Microscopy & Microanalysis 2018
Message: Dear Microscopists and Microanalysts, It is our pleasure to inform you of: Solid State X-ray Spectrometry at 50 Years, session A11 at the 2018 Microscopy & Microanalysis Meeting, which will be held Aug. 5-9, 2018 in Baltimore, MD.
This session will concentrate on the diverse advances in hardware, software, and applications of energy-dispersive spectrometry in the last 50 years.
We are particularly interested in applications from the biological and materials communities, especially from students and young scientists.
The MSA website link to submit a paper is now active. The deadline for submission is Feb. 15, 2018: https://www.microscopy.org/MandM/2018/
Solid State X-ray Spectrometry at 50 Years
Session chairs: Paul Carpenter, Edward Vicenzi, Kate Burgess, Nicholas Ritchie
50 years ago, Fitzgerald, Keil, and Heinrich published the first results obtained from an energy-dispersive x-ray spectrometer (EDS) in Science. From identification of unknown materials, to compositional mapping and quantitative microanalysis, EDS has advanced our understanding of an enormous range of materials and is used worldwide in microanalysis and microscopy laboratories. The symposium will link historical and technical developments of solid state x-ray instrumentation, data processing, applications, and emerging detection systems. Perspectives on the developments in EDS from a technological and educational perspective will be featured, including invited and contributed presentations from the inventor, vendor, and scientific communities.
o State-of-the-art solid state x-ray detector instrumentation o Soft x-ray and light element analysis in the field emission SEM o Nano to atomic resolution microanalysis by STEM o Quantitative analysis methods - limits and limitations o Spectrum imaging and data processing o Complementary methodologies including micro-XRF, combined WDS-EDS, XRD, and EBSD
Paul Carpenter, Washington University
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Email: klemari-at-fzu.cz Name: Mariana Klementova
Organization: Institute of Physics of the Czech Academy of Sciences
Title-Subject: [Filtered] Lorentz microscopy - position
Message: Dear colleagues,
we are currently looking for a microscopist who would be able to introduce Lorentz microscopy to our group at the Institute of Physics. We have a Tecnai F20 X-twin with a Lorentz lens. We have got funding for the position for 6 months. The position will be available from June 1, 2018.
If you are interested please contact me by January 31, 2018.
With best regards,
Mariana Klementova
************************************************ Mariana Klementova ************************************************ Institute of Physics of the CAS, v.v.i. Na Slovance 1999/2 CZ-182 21 Praha 8 Czech Republic ************************************************ tel. : +420 - 266 05 2612 email: klemari-at-fzu.cz ************************************************
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Email: kandi-at-isearchbio.com Name: Kandi Williams
Organization: K.L. Williams & Associates
Title-Subject: [Filtered] Entry level microscope sales - San Francisco and Seattle
Message: We are currently working with a major manufacturer of laboratory microscopes and related optical products to assist them in filling a San Francisco and a Seattle based entry level microscope sales positions. We are looking for a young, energetic scientist with a year or two of post doc experience, or several years post graduate lab management experience, with heavy confocal experience using, teaching, troubleshooting. This would be an excellent first step off the bench into industry. The company will provide excellent training and mentorship and a solid career path. First year salary package is anticipated to be around $100k plus the company provides a monthly car allowance, covers all business and travel expenses and has an excellent benefits package. Overnight business travel for both territories is low, maybe 2 to 3 nights/month, primarily for meetings and training. Please note that the company is unable to provide any sort of visa sponsorship at this time. Take care, Kandi Williams
K.L. Williams & Associates P.O. Box 5421 Auburn, CA 95604-5421 (530) 885-3693 kandi at isearchbio.com
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Hello, I recently purchased a Wig-L-Bug grinder/mixer, an agate vial and an adapter to hold the vial. I am at a loss as to how the vial is held by the adapter and the adapter certainly does not fit on the arms of the grinder/mixer. I made three attempts at contacting the manufacturer's technical support but to no avail. Hence this plea for help. If any of you have this setup, I appreciate your input. I can share photos of the parts if that would help.
BTW, the purpose for the agate vial is for grinding ashed materials that often contain silicates.
Tom Kremer SGS-IPS Testing Appleton, WI Information in this email and any attachments is confidential and intended solely for the use of the individual(s) to whom it is addressed or otherwise directed. Please note that any views or opinions presented in this email are solely those of the author and do not necessarily represent those of the Company. Finally, the recipient should check this email and any attachments for the presence of viruses. The Company accepts no liability for any damage caused by any virus transmitted by this email. All SGS services are rendered in accordance with the applicable SGS conditions of service available on request and accessible at http://www.sgs.com/en/Terms-and-Conditions.aspx
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Email: mplattsc-at-gmail.com Name: Michael Platt
Organization: University of South Carolina
Title-Subject: [Filtered] Vac check connection failure, Zeiss Leo SEM
Message: I have been working on this issue, along with an L-REM failure off and on for a while and have gotten nowhere. I have been concentrating on the L-REM failure but now I want to look harder into trying to solve the Vac Check Connection fail. Those failures show up during the power up initialization. First, what does that really mean? Not that it has anything to do with the failure but the backing pump and turbo pump both come on and I believe the turbo comes up to speed, solid green light on the controller. The vacuum gauge's green lamp is also on. Regarding previous suggestions I have check every fuse I could find, all good. All fiber optic cables appear to be OK on the L-REM and associated units. Tests indicate the power supply failures on the EO and PU boards but where exactly are they as I could find only numeric id's for all the PC boards.
Suggestions?
Michael Platt
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Title-Subject: [Filtered] The Rockefeller University seeks Electron Microscopy Specialist
Message: Message: Electron Microscopist, The Rockefeller University, New York, NY
The Rockefeller University, a premier biomedical research institution, seeks a Research Support-at-Associate or Specialist to join our Electron Microscopy Resource Center (EMRC).
The EMRC provides state-of-the-art electron microscopy support for analysis of a wide variety of biological samples, including viruses, bacteria, insects, animal tissue as well as cultured cells and isolated cellular components for structural analyses or immuno-electron microscopy. The EMRC is equipped with three transmission electron microscopes, a conventional and a serial block-face imaging scanning electron microscope, and a high-pressure freezing and a freeze-substitution unit. (http://www.rockefeller.edu/emrc/)
The Research Support Associate/Specialist will participate in all of the EMRCfs daily operations, including maintenance, upkeep and use of the electron microscopes and associated equipment, ordering supplies, interacting with vendors, and administrative support for office duties, including center billing. The position also entails specimen preparation, including negative staining, ultrathin sectioning, and immunolabeling, operation of the microscopes and associated equipment, training users, as well as consulting scientists on the design of experiments, data processing/analysis, interpretation of results, and informing users on the latest methodology through familiarity with relevant literature.
The successful candidate will have an M.S./Ph.D. degree or equivalent background in biology, bioengineering or a related field and must have a minimum of 5 years of hands-on experience in electron microscopy. A strong background in computation would be a plus. Must have strong communication skills, and the ability to work collaboratively in a team as well as independently on a wide variety of research projects. Must be detail-oriented, focused, and highly motivated.
We offer a competitive salary, comprehensive benefits, and a collegial work environment. To apply to this job, click the following URL, click on 'staff opportunitiesf and enter keywordeIRC20449f or eElectron Microscopistf: http://www.rockefeller.edu/hr/career.php
The Rockefeller University is an Affirmative Action/Equal Employment Opportunity/VEVRAA employer.
Regards, Hiro ------ Kunihiro Uryu, Ph.D., Research Assistant Professor Director of Electron Microscopy Resource Center (EMRC) RRB Rm120 The Rockefeller University 1230 York Ave., Box 230 New York, NY 10065
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Email: lamt-at-si.edu Name: Thomas Lam
Organization: Smithsonian Museum Conservation Institute
Title-Subject: [Filtered] P08 Spectroscopic and Imaging Studies in Heritage Science
Message: It is our pleasure to inform you there will be a Spectroscopic and Imaging Studies in Heritage Science symposium, session P08 at the 2018 Microscopy & Microanalysis Meeting, which will be held Aug. 5-9, 2018 in Baltimore, MD.
The MSA website link to submit a paper is now active. The deadline for submission is Feb. 15, 2018: https://www.microscopy.org/MandM/2018/
P08 Spectroscopic and Imaging Studies in Heritage Science
The application of microscale and nanoscale characterization techniques to the examination of cultural heritage materials has greatly enhanced our understanding of the processes that formed, and subsequently transformed those materials to their present state. Understanding the chemistry and morphology of heritage materials from the macro/mesoscopic scale to the microscale is of critical importance for our increasingly deeper levels of knowledge of the interaction between objects and their environment. This symposium will include invited and contributed presentations from students, conservators, conservation scientists, researchers, and those from other disciplines who have an interest in the preservation of cultural heritage.
o Emerging methods for microscale and nanoscale examination in culture heritage o Case studies on historic and prehistoric materials o Non-invasive and minimally-invasive imaging and analysis methods o Kinetics of light fastness using microfadeometry o Synchrotron- and neutron-based studies o New approaches to protective coatings for heritage objects ________________________________________
Students, postdocs, and technical staff All full-time students enrolled at accredited academic institutions and all full-time postdoctoral fellows are eligible for meeting award support. High school, undergraduate, and graduate students are encouraged to apply. Applicants are not required to be members of the sponsoring societies. Additionally, full-time technologists are eligible for support as well if the applicant is a member of the sponsoring society, current in his or her dues for the year of the meeting.
We look forward to seeing you at the Baltimore inner harbor next year! On behalf of the symposium organizers,
Edward P. Vicenzi (Smithsonian Institution) John Mansfield (University of Michigan) Thomas Lam (Smithsonian Institution)
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Organization: Northern California Society for Microscopy
Title-Subject: [Filtered] NCSM Spring Meeting in Pleasanton, CA
Message: Northern California Society for Microscopy announces its spring meeting on March 27 at the Zeiss Customer Center in Pleasanton, CA. MSA guest tour speaker is: Sara Miller, PhD. of Duke Medical Center. Presenting: Emerging Diseases and Microscopy
More info at: ncsmicroscopy.org
RSVP: info-at-ncsmicroscopy.org
We hope you will attend.
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Name: Janine Hernandez School: San Joaquin Delta College Grade/Education Level: Undergraduate Location: Stockton, CA Email: janine.hernandez182-at-gmail.com
(Listserve - I apologize - I think I did the reply incorrectly at first)
Hi Janine,
Powders and pigments likely do not require encapsulation. It's usually much easier to place them on a supporting grid directly for imaging. This of course assumes that the size of the powder or pigment is small enough for electron transmission. Often it's worth it just to check incase it is possible; which makes sample prep fairly easy.
There are a few methods that could be used to prepare these- one that I've had some success with fine powders in the past is as follows: place an amount of the powder into isopropanol, ultrasonicate to break up agglomerations, dip a holy-carbon grid into the liquid, pull it out, and allowing the grid to air dry. If there was a small enough amount, but enough to be finely dispersed in the alcohol, you will pick up some on the grid. This grid, when dried, can then be imaged with the powders suspended on the holy-carbon. It may take a few trials to get enough from the alcohol suspension. Another method that has worked for me in the past (assuming the powder is well separated) is a very light dusting of the powder mid-air above the grid (allowing some of the powder to fall down onto the grid on the table). This one is less likely to obtain separated powders that are small enough to image, but I have seen some success with it as well in the past. (Use of a clean fine artist paint brush to flick the powder into the air above the grid may work here.) Please be careful not to allow the alcohol with the powder or the powder in air to get on your skin or to be breathed in. Some solvents (acetone notably) allows chemicals to enter through the skin, and we are learning that nanopowders are not healthy for you to breathe in as well.
I hope this is helpful. You'll likely get a number of great answers from the list-serve and I'm looking forward to learning from their answers also!
Wishing you a grand time exploring small worlds! -Allen J. Hall Prairie Nanotechnology www.prairienanotech.com
Senior Postdoctoral Fellow in the Canadian Centre for Electron Microscopy
The Canadian Centre for Electron Microscopy (CCEM) is a national facility located at McMaster University, in Hamilton, ON, Canada. The CCEM operates four TEMs, including two FEI Titan TEMs, equipped with image and probe correctors and monochromators, as well as a Quantum GIF equipped with K2 direct electron detector for spectroscopy.
We are seeking a skilled and motivated researcher to perform a variety of imaging and analysis experiments supporting the needs of academic and industrial users, including consulting, planning of experiments, data processing and interpretation. An important role of this position is the training of users on the instruments.
Candidates should have a PhD in Physics, Material Science/Engineering, Chemistry or a related field with demonstrated hands-on experience using transmission electron microscopes and EDS/EELS, preferrably Titan TEMs. Strong communication skills and the ability to develop successful data acquisition and processing strategies will be essential in this role. Experience in data processing and scripting in Python or Matlab, as well as a demonstrated track record of research and interactions with users would be an asset.
To apply, please send your cover letter and resume to korinek-at-mcmaster. ca
-- Dr. Andreas Korinek
Manager
Canadian Centre for Electron Microscopy Brockhouse Institute for Materials Research McMaster University 1280 Main Street West, Hamilton ON Canada, L8S 4M1 phone: +1 905-525-9140 ext 20400
==============================Original Headers============================== 10, 38 -- From korinek-at-mcmaster.ca Mon Jan 29 15:19:13 2018 10, 38 -- Received: from FHSHC4H16-2.csu.mcmaster.ca (fhshc4h16-2.CSU.McMaster.CA [130.113.22.4]) 10, 38 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w0TLJD6e030414 10, 38 -- for {microscopy-at-microscopy.com} ; Mon, 29 Jan 2018 15:19:13 -0600 10, 38 -- Received: from FHSDB2D11-2.csu.mcmaster.ca ([fe80::2924:2ca:3cbc:4521]) by 10, 38 -- FHSHC4H16-2.csu.mcmaster.ca ([2002:8271:1604::8271:1604]) with mapi id 10, 38 -- 14.03.0319.002; Mon, 29 Jan 2018 15:14:11 -0500 10, 38 -- From: "Korinek, Andreas" {korinek-at-mcmaster.ca} 10, 38 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 10, 38 -- Subject: Job Opening: Senior Postdoctoral Fellow in the Canadian Centre for 10, 38 -- Electron Microscopy 10, 38 -- Thread-Topic: Job Opening: Senior Postdoctoral Fellow in the Canadian Centre 10, 38 -- for Electron Microscopy 10, 38 -- Thread-Index: AQHTmT25Tf/tzryPHUm6S/U4i2qljQ== 10, 38 -- Date: Mon, 29 Jan 2018 20:14:10 +0000 10, 38 -- Message-ID: {1517256849.24146.8.camel-at-mcmaster.ca} 10, 38 -- Accept-Language: en-CA, en-US 10, 38 -- Content-Language: en-US 10, 38 -- X-MS-Has-Attach: 10, 38 -- X-MS-TNEF-Correlator: 10, 38 -- x-originating-ip: [130.113.22.232] 10, 38 -- x-tm-as-product-ver: SMEX-12.5.0.1300-8.2.1013-23628.002 10, 38 -- x-tm-as-result: No-5.608800-5.000000-10 10, 38 -- x-tmase-matchedrid: SbnkZF0UrmA/mJEvNFL+dY+emiGnyeDR4Pjj54kIW/GVvB3MWg1bhKjP 10, 38 -- dtRFsI/Ttpq8RF54CmTUqlNduJHbX6ZJF6TEqRucQjDDVgDayL60xIzVr7Ulb0eD57cU53Gilpf 10, 38 -- gNtKoBw6qYaQyxONnx5Zd8LXqzTgbPHPcxToV+PdCZSbGz8XXg1z85UQdvE4EFNu748/lj/LCBr 10, 38 -- VgCBNlqSVyXfqCUtLRSy+kn1dQUY2yl+gKyy/g7xyFKiDPvSEg/kqO2xYqX5Y7g4/7QP0AlqPFj 10, 38 -- JEFr+olA6QGdvwfwZbkwjHXXC/4I8prJP8FBOIavByPQY4m0tDBOwgZ87U4NKl3cNQsLUXRoR/L 10, 38 -- CWpMc6W2H0DwvnL5XsC+ksT6a9fy 10, 38 -- x-tm-as-user-approved-sender: No 10, 38 -- x-tm-as-user-blocked-sender: No 10, 38 -- x-tmase-result: 10--5.608800-5.000000 10, 38 -- x-tmase-version: SMEX-12.5.0.1300-8.2.1013-23628.002 10, 38 -- Content-Type: text/plain; charset="utf-8" 10, 38 -- Content-ID: {8FEBFC9E8893F64886477620D22C54C5-at-fhsadmin.csu.mcmaster.ca} 10, 38 -- MIME-Version: 1.0 10, 38 -- Content-Transfer-Encoding: 8bit 10, 38 -- X-MIME-Autoconverted: from base64 to 8bit by microscopy.com id w0TLJD6e030414 ==============================End of - Headers==============================
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Email: psicurello-at-ucsd.edu Name: Paula Sicurello
Organization: UCSD
Title-Subject: [Filtered] Zeiss 10C available
Message: Please post for me this Zeiss 10C that we are giving away.
Hello Everybody,
Free to a good home.
Is anyone interested in a vintage Zeiss 10C? This scope is in working condition and has been lovingly maintained. It comes with a side mounted Gatan ES1000W camera. The purchaser will be responsible for the costs associated with the disassembly and removal.
Please contact me if you are interested.
Sincerely,
Paula Sicurello, HTL (ASCP)
Histotechnology Specialist
UC San Diego Health
200 Arbor Drive
San Diego, CA 92103
psicurello-at-ucsd.edu P: 619-583-2872
Sincerely,
Paula Sicurello, HTL (ASCP)CM
Histotechnology Specialist
UC San Diego Health 200 Arbor Drive
San Diego, CA 92103
(P): 619-543-2872
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Email: cjbray-at-es.utoronto.ca Name: Colin Bray
Organization: Earth Science, Univ. of Toronto
Title-Subject: [Filtered] ETEC Autoscan SEM circuit diagrams needed
Message: A colleague has an ETEC Autoscan SEM with an EDS and is need of a circuit diagram for the vacuum system driver board. he has circuit diagrams for most of the other units but naturally, not the one he needs. Can anyone help?
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Dear colleagues - to commemorate the decade of PFM conferences, I have made the integrated set of slides (~350) on the principles and applications of piezoresponse force microscopy and spectroscopy based on tutorials on ~20 PFM workshops and conferences over last decade.
There are 7 parts, including: 1. Introduction to PFM and Nanoscale electromechanical phenomena 2. Contact mechanics and image formation in PFM 3. Cantilever dynamics in PFM 4. PFM of ferroelectric materials and local polarization switching 5. Switching spectroscopy piezoresponse force microscopy 6. Advanced time- and voltage spectroscopies in PFM 7. PFM in polar and nonpolar liquids
If interested, please contact me directly at sergei2-at-ornl.gov {mailto:sergei2-at-ornl.gov} . Also, please forward this information to your colleagues interested in PFM.
Sergei
-- Sergei V. Kalinin
Director, Institute for Functional Imaging of Materials
Fellow MRS, IEEE, APS, IoP, AVS
Oak Ridge National Laboratory
Phone: (865) 241-0236
==============================Original Headers============================== 9, 37 -- From sergei2-at-ornl.gov Wed Jan 31 10:54:42 2018 9, 37 -- Received: from mta02.ornl.gov (mta02.ornl.gov [128.219.177.136]) 9, 37 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w0VGsfQH003732 9, 37 -- for {microscopy-at-microscopy.com} ; Wed, 31 Jan 2018 10:54:42 -0600 9, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 9, 37 -- d=ornl.gov; i=-at-ornl.gov; q=dns/txt; s=p20151116; 9, 37 -- t=1517413785; x=1548949785; 9, 37 -- h=to:from:subject:message-id:date:mime-version: 9, 37 -- content-transfer-encoding; 9, 37 -- bh=WK+xz8dtT86FfuyGPtcrR3ftshOsu+zFz8iEyFxfATc=; 9, 37 -- b=OCYQOz85CMSU7QUTt9NfeT+/Nv/sqdS4jpaL/U0hrOxdMoCzX42sMvtA 9, 37 -- vvZEYX1p+KdQfY/tgJMqN71r5xI1FDs0UVsy7XwmTFmIBx6ekNEHOBcrZ 9, 37 -- p0GfJ4axuwlnglWq1ZLTJasA/Ac47IL4RYoUa6c3FkMteCM5GVUejmWmz 9, 37 -- +DvMlf9gZIWqCphesnE3Y7M8rn0tzhW/jHKP658Eopz3mtttuLcW6lS4B 9, 37 -- W8uX1WVK6kluGpbwJ3rVVAimy3Kumio0dJZ02XF2oUdH3Y39pa6o9Wfai 9, 37 -- quwezOH12djQDjmyVc334jGkZCYMHQXb6TqQ8CG6qOuHDwQmZQdBIBuP2 9, 37 -- w==; 9, 37 -- X-SG: RELAYLIST 9, 37 -- X-IronPort-AV: E=Sophos;i="5.46,440,1511845200"; 9, 37 -- d="scan'208";a="31016179" 9, 37 -- Received: from emgwy2.ornl.gov ([160.91.254.10]) 9, 37 -- by iron2.ornl.gov with ESMTP/TLS/DHE-RSA-AES256-GCM-SHA384; 31 Jan 2018 10:49:45 -0500 9, 37 -- Received: from [128.219.192.116] (pc95228.ornl.gov [128.219.192.116]) 9, 37 -- (using TLSv1.2 with cipher ECDHE-RSA-AES128-GCM-SHA256 (128/128 bits)) 9, 37 -- (No client certificate requested) 9, 37 -- by emgwy2.ornl.gov (Postfix) with ESMTPS id 3zWnkK28c7z2TF1s 9, 37 -- for {microscopy-at-microscopy.com} ; Wed, 31 Jan 2018 10:49:45 -0500 (EST) 9, 37 -- To: microscopy-at-microscopy.com 9, 37 -- From: "Sergei V. Kalinin" {sergei2-at-ornl.gov} 9, 37 -- Subject: Piezoresponse Force Microscopy tutorial - integrated slides 9, 37 -- Message-ID: {5A71E598.8000501-at-ornl.gov} 9, 37 -- Date: Wed, 31 Jan 2018 10:49:44 -0500 9, 37 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:38.0) Gecko/20100101 9, 37 -- Thunderbird/38.0.1 9, 37 -- MIME-Version: 1.0 9, 37 -- Content-Type: text/plain; charset=utf-8; format=flowed 9, 37 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Ken Converse used to support ETECs, the bits of contact info I have are:
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz {mailto:kenconverse-at-qualityimages.biz} qualityimages.biz
Quality Images, /Ken Converse/, Maine, from NC to ME to OH, SEM, Amray//Etec//Any, (717) 456-5491
Good luck! Valery Valery Ray ============================== PBS&T, MEO Engineering Company 290 Broadway, Suite 298 Methuen, MA 01844, USA Phone: +1-978-305-0479 - leave a message Mobie: +1-978-305-0479 - leave a message E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
---------------------------------------------------------------------------------------------------- *From:* "microscopy.listserver-at-gmail.com" {microscopy.listserver-at-gmail.com} *To:* vray-at-partbeamsystech.com *Sent:* Wednesday, January 31, 2018 9:08 AM *Subject:* [Microscopy] viaWWW: ETEC Autoscan SEM circuit diagrams needed
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Email: cjbray-at-es.utoronto.ca {mailto:cjbray-at-es.utoronto.ca} Name: Colin Bray
Organization: Earth Science, Univ. of Toronto
Title-Subject: [Filtered] ETEC Autoscan SEM circuit diagrams needed
Message: A colleague has an ETEC Autoscan SEM with an EDS and is need of a circuit diagram for the vacuum system driver board. he has circuit diagrams for most of the other units but naturally, not the one he needs. Can anyone help?
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Email: tstern-at-princeton.edu Name: Tomer Stern
Organization: Princeton University
Title-Subject: [Filtered] Beads size and intensity
Message: Hi all,
I'm using a MuVi-SPIM, similar to the Luxendo model.
To register the different views and also to infer the PSF prior to deconvolution I'm using beads, which are scattered around the sample - inside the agarose - and are being scanned at the same time as the sample.
I have two questions:
1. Is the intensity of the beads relative to the intensity of the marker inside the tissue important for the inference of the PSF? Meaning, is it better to use beads with intensities that are similar to the sample, or should their intensity be higher / lower / it doesn't really matter?
2. As far as I know the preferred diameter of the bead is one that is a bit smaller than the size of the voxel in the XY plane, right? if so, then if I know that I'm going to use a 2x2 binning simply by averaging every 2x2 block in each slide, should I still use the same size of beads, or should it match the new size of the 'binned' - enlarged - pixels?
Thanks in advance! Tomer
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Berkeley Lab’s Molecular Foundry Division has an opening for a Scientific Engineering Associate or Sr. Scientific Engineering Associate. The Molecular Foundry is a Department of Energy-funded nanoscience research facility that provides users from around the world with access to cutting-edge expertise and instrumentation in a collaborative, multidisciplinary environment. As one of the seven facilities in the Molecular Foundry, The National Center for Electron Microscopy (NCEM) serves a broad user community in materials science by supplying unique instrumentation, expert advice, assistance, and training in advanced electron microscopy techniques.
Diversity, equity, and inclusion are core values at the Molecular Foundry. Therefore, we seek candidates whose research, past employment, or service has prepared them to contribute to our commitment to diversity and inclusion. Our excellence can only be fully realized by staff who share our commitment to these values. Successful candidates for our staff position will demonstrate either potential to contribute or evidence of a commitment to equity and inclusion.
This position provides highly specialized expertise, consultation, and contributions to project process and knowledge in support of in situ transmission electron microscopy (TEM) experimentation and TEM sample preparation. This includes the development and optimization of techniques for preparing high resolution TEM samples from a wide variety of materials and for in situ TEM investigations, user assistance and training, technical proposal evaluation, and collaborations on scientific projects. The position is responsible for scheduling, maintenance, operation and technical development of the sample preparation laboratories and in situ TEM experimental equipment. Will provide resolution to a diverse range of moderately complex technical problems and participate in, and in some cases lead, experimental methodology development and project execution. Classification will depend upon the applicant's level of skills, knowledge, and abilities.
For further details and to apply please go to the following link:
http://m.rfer.us/LBLYNuJJ
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Title-Subject: [Filtered] Postdoctoral Fellow In situ cryo-EM
Message: Dear list members,
In my lab, a position of in situ cryo-EM in the area of structural cell biology is available. I am still looking for a candidate with experience in cryo-ET/EM and/or FIB-SEM for the following project: http://s.embl.org/HD01243
In case you have further questions, please do not hesitate to contact me. Closing date for applications is Feb 16.
Best wishes,
Carsten ____________________________________________________________________________________ Dr. Carsten Sachse Group Leader European Molecular Biology Laboratory Meyerhofstr. 1 69117 Heidelberg Germany email: carsten.sachse-at-embl.de http://www.embl.de/research/units/scb/sachse/ http://www.sachse.embl.de
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Hello Everyone, I'm using a Philips CM12 and I've been noticing recently I still have a beam and image with the filament supposedly desaturated. I have to turn the high voltage off to get the beam completely gone. I don't remember this scope doing this before. So, I suspect something isn't right but before I request a service call (we are a fugal company) I'd like to confirm that the scope is acting abnormally. I'm also fishing for ideas on what is wrong.
Of course who can't resist wondering where all those people out of the office are and is it warmer than Akron Ohio in February!
Thanks!!!
Stay safe...........
Frank Karl Microscopist Akron Rubber Development Laboratory 2887 Gilchrist Road Akron, Ohio 44305
I've had a similar issue with our FEI Morgagni 267D (for most purposes similar to the CM12). I'd bet the wehnalt assembly is almost identical. For us it was almost certainly a filament break in such a way that it was shorting across the wehnalt. I say "almost certainly" because in our case this happened at almost the same time that a bunch of electronics issues occurred and so we had mixed symptoms. But replacing the filament (which we had only just replaced as part of our diagnosis of elimination), did the trick for us.
At least it is a simple check.
Good luck, Duane
Duane Harland Senior Scientist Food & Bio-based Products T +64 3 321 8710 Based at Lincoln Research Centre Campus agresearch.co.nz New Zealand
-----Original Message----- X-from: frank_karl-at-ardl.com [mailto:frank_karl-at-ardl.com] Sent: Thursday, 8 February 2018 9:10 AM To: Harland, Duane {Duane.Harland-at-agresearch.co.nz}
Hello Everyone, I'm using a Philips CM12 and I've been noticing recently I still have a beam and image with the filament supposedly desaturated. I have to turn the high voltage off to get the beam completely gone. I don't remember this scope doing this before. So, I suspect something isn't right but before I request a service call (we are a fugal company) I'd like to confirm that the scope is acting abnormally. I'm also fishing for ideas on what is wrong.
Of course who can't resist wondering where all those people out of the office are and is it warmer than Akron Ohio in February!
Thanks!!!
Stay safe...........
Frank Karl Microscopist Akron Rubber Development Laboratory 2887 Gilchrist Road Akron, Ohio 44305
Frank, This is called Dark Current. It comes from the evaporation of filament material onto the insulator base creating a pathway for electrons to leak to ground, and therefore, creating a current in the filament that causes it to glow with only the kV on. It’s not a problem, but indicates that your filament is old. Ken
Kenneth JT Livi, PhD Director, Materials Characterization and Processing Center Materials Science and Engineering 3400 N Charles Street Johns Hopkins University Baltimore, Maryland 21218 USA
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A collegue in the lab has strong health difficulties with her shoulders and wrists. She works on a Zeiss Axio Imager D2m with a special stage and external accessories which leaves only a close access to the focusing knobs. So we are looking for a companies which could fit a motorization on the focus knobs, to have a remote control of them. Does someone know a (preferably french or europeen) company which could do this job ? We'll ask Zeiss too, but we have some fear about the price...
Thanks in advance Jacques
--
J. Faerber IPCMS-DSI Institut de Physique et Chimie des Matériaux de Strasbourg Département Surfaces et Interfaces 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
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Email: gary-at-cermetmaterials.com Name: Gary Castelow
Organization: CERMET MATERIALS INC.
Title-Subject: [Filtered] JEOL 840A SEM
Message: Hello all! We randomly lost video signal with our SEM the other day. Acted as if the filament had blown, but the check gauge is still reading current when the AC is turned on. Im am by no means an "electrician" but all connections still look ok going to the viewing monitor. Is there something fairly easy that goes bad I could check?? Or any other help is greatly appreciated!
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Email: wschneider-at-wisc.edu Name: Bil Schneider Organization: UW Madison Title-Subject: [Filtered] Tungsten filaments
Message: We use tungsten filaments in our Hitachi S3400N VPSEM. Does anyone have any suggestions for extending filament life? We try and operate slightly under saturated except for EBSD or EDS mapping. Ive heard of seasoning the filament but have never seen an actual protocol or talked with anybody whos actually done it. Any suggestions would be appreciated!
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did you check your complete video-processing path, like:
- setting the gain of the SE-detector so high to finally see the signal noise (this would state at least a working photomultiplier / detector system)
- did you check for the cage voltage switch to be on "positive" ?
- check on HV voltage and cathode heating. Do you see any change by going up in heating?
- centering and e-beam shift potis aligned?
- cathode OK? Current might be a dark currant or mis-functioning high voltage unit
- take out the final aperture and try to find beam...
These are only a few possibilities...
Best wishes,
Stefan
-
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Bill, with " } seasoning { a tungsten filament" you perhaps (might) think of slow and careful -controlled 'heating' of a new filament (as I have done that with every new filament after mounting in a TEM, a ZEISS109 with differential pumping system=rotary pump -for RV as well as an Ion Getter Pump for HV).
IMHO 'slow heating' of the cathode filament before subjecting it to the "hard" working conditions might be (at least in my experience really 'is' ) of benefit regarding beam stability as well as maintaining your filament's lifetime.
"Slow heating" means [after change of filament] at least running the new filament 1-2 hrs 'under-under'-saturated after achievement of an evacuation optimum of the chamber (not knowing whether you could see anyhow the filament's image in such a condition like in a TEM on the screen). You easily would / could see (if you e.g. have permanent vacuum-measurement monitoring ready) that on starting to "heat"(increase) the filament (current), the vacuum decreases significantly (at least slightly) because of "evaporation" of metal & impurities / contaminants.
Then increase filament current a bit more.... and let "equilibrate" vacuum conditions. You might even find such decreasing vacuum after a third increasing of the filament heating near the 'saturation' point.
Another helpful requisite also would be to use - without using or even heating the filament first an ACD (anti-contamination device - if available) with at least one if not two cycles of cooling first and pumping the chamber afterwards to get rid of most organic molecules you brought into the filament chamber by your cleaning and mounting procedures - let warm up the ACD and pump (RV and HV if possible) again. According to the specifications of the Hitachi S3400N VPSEM (I found on: https://www.ntnu.edu/documents/140082/1269041159/S-3400NSpecifications.pdf/6 b94c26f-a9d4-4f36-82b3-7e9eb3603922 ) the possibility of doing so might be impossible due to the automatic settings of diverse functions but you should know about those possibilities from practical experience.
Unfortunately a modern VPSEM is not comparable to such an old piece of equipment like the TEM ZEISS 109 (vintage 1976) I worked with where maintaining and handling vacuum matters relatively easy... And, BTW, life time of a tungsten filament always depends also on the "technical quality" and the handling - when mounting into the chamber - too....
Best wishes and good luck, Wolfgang
MUSS Wolfgang Dr. phil. (PhD) [OR i. R. / en retraite / retired] A-5020 SALZBURG sterreich-AUSTRIA Mobile-Tel.: 0043(0)676 5 369 456 E-mail: wij.muss-at-aon.at E-Mail altern.: womuss-at-gmail.com
FRMS, Retired Member of MSA Scientific Profile at ResearchGate: http://www.researchgate.net/profile/Wolfgang_MUSS Former Head of Electron Microscopy Lab at Institute of Pathology SALK-LKH / Salzburger Landeskliniken | General Hospital and PMU (private) PARACELSUS MEDICAL UNIVERSITY of SALZBURG
============================================================================ ================================= Von: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Gesendet: Freitag, 9. Februar 2018 10:29 An: wij.muss-at-aon.at Betreff: [Microscopy] Tungsten filaments [Hitachi S3400N VPSEM, how extending filament life?] ---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
please copy both wschneider-at-wisc.edu as well as the Microscopy Listserver --------------------------------------------------------------------------- Email: wschneider-at-wisc.edu Name: Bil Schneider Organization: UW Madison Title-Subject: Tungsten filaments Message:
We use tungsten filaments in our Hitachi S3400N VPSEM. Does anyone have any suggestions for extending filament life? We try and operate slightly under saturated except for EBSD or EDS mapping. Ive heard of seasoning the filament but have never seen an actual protocol or talked with anybody whos actually done it. Any suggestions would be appreciated!
First, what kind of filament life are you currently getting? If you are close to 100 hours, I would be satisfied. If you are only getting 30, then I would look for a problem.
We got better life when we ran at a consistent voltage and didn't have users doing the saturating. We went from average lifetimes of 25 hours to 80 hours. We also operated just under saturation conditions. We simply turned off the high voltage at the end of the session.
We needed to check saturation and reduce current over time. As the filament thinned, it needed less current to reach the same temperature.
Finally, we ran the filament a little further back from the front of the wehnelt. That lessened brightness but increased life.
Warren ________________________________________ X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Friday, February 9, 2018 3:09:18 AM To: Straszheim, Warren E [BIOTC]
X-from: wschneider-at-wisc.edu
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Email: wschneider-at-wisc.edu Name: Bil Schneider Organization: UW Madison Title-Subject: [Filtered] Tungsten filaments
Message: We use tungsten filaments in our Hitachi S3400N VPSEM. Does anyone have any suggestions for extending filament life? We try and operate slightly under saturated except for EBSD or EDS mapping. I've heard of "seasoning" the filament but have never seen an actual protocol or talked with anybody who's actually done it. Any suggestions would be appreciated!
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Name:karl Hagglund School:Northwestern University Grade/Education Level:Graduate Location:Evanston, IL US Email:karl.hagglund-at-Northwestern.edu {mailto:karl.hagglund-at-Northwestern.eduSubject} Subject {mailto:karl.hagglund-at-Northwestern.eduSubject} :Parts Needed Leo/Zeiss 1525 imaging board Your Question: Our Leo 1525 has suffered a failure on the imaging board resident on its vintage Windows 98 PC. This is a key system on the microscope, and we have learned that Zeiss no longer has replacements available, and there is not a current computer upgrade path. If there a user out there who might have an old PC is the cupboard from their Vintage Zeiss/Leo SEM, I would be very interested in hearing about it. We are looking for replacement boards for our 20 year old LEO PC. These systems have frame grabbing and scan control boards resident on the system computers, and we are in need of replacement parts. Thank you, Karl Hagglund
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Hi Karl, If you're in a rush, I would start by contacting these after-market providers: http://www.jcnabity.com/links.htm#Digital Imaging
If you aren't in a rush, I would find an Electrical Engineering student who wants a long-term project (lots of EE programs have this as a graduation requirement) and get them to build one for you (and ideally require they publish their methods openly). They can start with this for inspiration and basics:
Electron microscope image capture with an oscilloscope https://www.youtube.com/watch?v=SWVu-qPR-Ws
Electron microscope image capture via microcontroller (with drill bit animation) https://www.youtube.com/watch?v=ruuxn2u3yao http://benkrasnow.blogspot.com/2015/08/drill-bit-in-electron-microscope.html
DIY Scanning Electron Microscope - Sources, Costs and References http://benkrasnow.blogspot.com/2011/03/diy-scanning-electron-microscope_26.html
SEM data files and image generation script http://benkrasnow.blogspot.com/2014/11/sem-data-files-and-image-generation.html
On Fri, Feb 9, 2018 at 3:18 PM, {oshel1pe-at-cmich.edu} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } *********************************************************************************** } Forwarded from "Ask a Microscopist" } Please remember that the person asking the question is likely } not a member the listserver, and } **any reply should go directly to the poster** } as well as to the list. } Using the "reply" function in your email does *not* send your answer } to the person asking the question. } Please copy their email address from their question. } *********************************************************************************** } } } } } } } Name:karl Hagglund } School:Northwestern University } Grade/Education Level:Graduate } Location:Evanston, IL } US Email:karl.hagglund-at-Northwestern.edu {mailto:karl.hagglund-at-Northwestern.eduSubject} } Subject {mailto:karl.hagglund-at-Northwestern.eduSubject} :Parts Needed Leo/Zeiss 1525 imaging board } Your Question: } Our Leo 1525 has suffered a failure on the imaging board resident on its vintage Windows 98 PC. This is a key system on the microscope, and we have learned that Zeiss no longer has replacements available, and there is not a current computer upgrade path. If there a user out there who might have an old PC is the cupboard from their Vintage Zeiss/Leo SEM, I would be very interested in hearing about it. We are looking for replacement boards for our 20 year old LEO PC. These systems have frame grabbing and scan control boards resident on the system computers, and we are in need of replacement parts. } Thank you, } Karl Hagglund } } } } } } ==============================Original Headers============================== } 10, 59 -- From oshel1pe-at-cmich.edu Fri Feb 9 16:58:07 2018 } 10, 59 -- Received: from NAM01-BN3-obe.outbound.protection.outlook.com (mail-bn3nam01on0113.outbound.protection.outlook.com [104.47.33.113]) } 10, 59 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w19Mw62x009150 } 10, 59 -- for {microscopy-at-microscopy.com} ; Fri, 9 Feb 2018 16:58:07 -0600 } 10, 59 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 10, 59 -- d=CentralMichigan.onmicrosoft.com; s=selector1-cmich-edu; } 10, 59 -- h=From:Date:Subject:Message-ID:Content-Type:MIME-Version; } 10, 59 -- bh=UKlhs70T8N2RchOoD5XEkqH/C+5CkuB8zI2H8YME/7A=; } 10, 59 -- b=OgcR5ZQlZB2cg+Lc6nMDHbX6DkhW7M4aONwtOxdVLewFCgsiaHth+fj0YCWJArD0hez8aScoYPyEYAu/KQoV5ghtez33mhoajIdyA2HS8ebvmJ8L6qqXhifJsk3JV6qes9Q6xcUuKxbS84wscL0olahXsoPYxBx/pYrk9x57rW0= } 10, 59 -- Received: from CY1PR0501MB1676.namprd05.prod.outlook.com (10.163.140.15) by } 10, 59 -- CY1PR0501MB1386.namprd05.prod.outlook.com (10.160.148.140) with Microsoft } 10, 59 -- SMTP Server (version=TLS1_2, } 10, 59 -- cipher=TLS_ECDHE_RSA_WITH_AES_256_CBC_SHA384_P256) id 15.20.485.3; 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-- -Nathan
==============================Original Headers============================== 13, 47 -- From nmz787-at-gmail.com Fri Feb 9 17:55:34 2018 13, 47 -- Received: from mail-pg0-f65.google.com (mail-pg0-f65.google.com [74.125.83.65]) 13, 47 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w19NtYjt004471 13, 47 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Feb 2018 17:55:34 -0600 13, 47 -- Received: by mail-pg0-f65.google.com with SMTP id t4so3515637pgp.8 13, 47 -- for {Microscopy-at-microscopy.com} ; Fri, 09 Feb 2018 14:51:08 -0800 (PST) 13, 47 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 13, 47 -- d=gmail.com; s=20161025; 13, 47 -- h=mime-version:in-reply-to:references:from:date:message-id:subject:to 13, 47 -- :content-transfer-encoding; 13, 47 -- bh=1guh5Wa2GBJEuLGDKB/Nj/MQijUEXpp4rJ0ZVHmFo8g=; 13, 47 -- b=P/ZQlkbBEju7uM6Vu4HyR5fV51Iy/GTNIB4VyhFGIfdYBkZ9qy38oQcfrfM4BO4g5F 13, 47 -- kCpq+uFTZnuB0okV/iDVfu4sTkibLlmF7JpwZPZmkPsr6lIr++ntPXLlDG5N0WIJotwT 13, 47 -- FX0vLmalzvSiBHe7ClA2CAKugShaKbvqdfTHv9U5SonUjsP6O462s5WOpEfcsUAFasPy 13, 47 -- 4JODbg9B71WW3OzNsZKFJEE54uSnuD9h5Uxc5ZtmGn2mFMbqodIQch3NLtANuapLZHBV 13, 47 -- M0HYxpEw3N5esbul4SMFECURKWaCXF2esKwaZhKKKApRq/pyZQwGy8TlOJuGXhdz2IQA 13, 47 -- yqyg== 13, 47 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 13, 47 -- d=1e100.net; s=20161025; 13, 47 -- h=x-gm-message-state:mime-version:in-reply-to:references:from:date 13, 47 -- :message-id:subject:to:content-transfer-encoding; 13, 47 -- bh=1guh5Wa2GBJEuLGDKB/Nj/MQijUEXpp4rJ0ZVHmFo8g=; 13, 47 -- b=csT3EmQjiwQ5bTmSbeNrhF1QqOKMSJAFSFM7oYtJMUUZZI1UQ5rHRTv4ns7w4oH12Z 13, 47 -- pNck+znOHmTMzAZyP4WVclA+sNUbZkUCwlqqWAH2sFzLSOHnIXo+B/4D+EDvhk3daRg1 13, 47 -- gzibioGUc9t3TlCjjI+d/Z2LvsXYscddkd5HT1uVk0YNLGskaRPAf4lES/uECpZnk7co 13, 47 -- mWu5Zl14fQqYu7fB7peEPVzraKvy1obomk9sO7Chcy1EVXm6VKhwFON4Oie/N3TSanhq 13, 47 -- GQhRwkJ1n9U5kY+lNTbKOzTmrh6W/2cv1hj+JEJ/n7l8LoB98b+oUhggNrhlvIAK7EOR 13, 47 -- zVcQ== 13, 47 -- X-Gm-Message-State: APf1xPDrrr6FTGilHDGWSGKhEmk5MU/rr+KTL3I1/HEnDxwnLcW20Aw8 13, 47 -- tUPBMo9YUBOWFhW5p6I/EnN6WX4PIu5THqaKXNE= 13, 47 -- X-Google-Smtp-Source: AH8x224ktEmBVqTIuSa7Q3XAUCK82HzaJjrwbHpA4668kY/I8jT+6++GKQPIiDdjaEmuXvUXdJVFMOWXm3eq9JuIL0k= 13, 47 -- X-Received: by 10.101.67.71 with SMTP id k7mr3512435pgq.136.1518216667533; 13, 47 -- Fri, 09 Feb 2018 14:51:07 -0800 (PST) 13, 47 -- MIME-Version: 1.0 13, 47 -- Received: by 10.100.138.10 with HTTP; Fri, 9 Feb 2018 14:50:46 -0800 (PST) 13, 47 -- In-Reply-To: {201802092318.w19NIgtM028746-at-microscopy.com} 13, 47 -- References: {201802092318.w19NIgtM028746-at-microscopy.com} 13, 47 -- From: Nathan McCorkle {nmz787-at-gmail.com} 13, 47 -- Date: Fri, 9 Feb 2018 14:50:46 -0800 13, 47 -- Message-ID: {CA+82U9Lsemg7dKd6dtPOykrpSNUutDqtkpc7yRF1CDSd8Nwv2g-at-mail.gmail.com} 13, 47 -- Subject: Re: [Microscopy] FW: Ask a Microscopist - Imaging board needed for a 13, 47 -- Leo 1525 13, 47 -- To: karl.hagglund-at-northwestern.edu, oshel1pe-at-cmich.edu, 13, 47 -- Microscopy-at-microscopy.com 13, 47 -- Content-Type: text/plain; charset="UTF-8" 13, 47 -- Content-Transfer-Encoding: 8bit 13, 47 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id w19NtYjt004471 ==============================End of - Headers==============================
Do you have a high vacuum gauge on the scope? With both our new Hitachi (SU3500) and our old JEOL (5600), the "ready" state comes on when the vacuum is in the high 10^-4 torr range (or even higher if there was a vacuum leak, outgassing sample, or other problem). Running a filament at such a poor vacuum will drastically reduce filament life and generally contaminate the column faster. I insist on having a gauge on any scope I run, and don't turn the beam on until the vacuum is at least mid 10^-5. With vacuum gauges under $1000, they'll quickly pay for themselves in terms of filaments and time lost changing them. I've ranted to both JEOL and Hitachi that they should make them standard on all scopes. By their own admission, half of their service calls are vacuum related, and with a gauge the end user can often find a leak and save a service visit. In our case with the closest service in Ontario, it's a $2000 flight to get here. Should be a simple budgetary calculation, but Hitachi says I'm the only customer in Canada who ever requested a vacuum gauge.
I also let the filament cool 5 to 10 minutes whenever possible before venting. I don't have any hard evidence, but it just seems logical to me that exposing a hot tungsten filament to atmosphere is going to induce more oxidation or whatever compared to a cool one.
In my experience, these precautions contribute more to filament life than slight undersaturation. Of course, you want to make sure you're not oversaturated, but I get 3-400 hours of the Hitachi filaments running in the "medium" gun setting.
Hope this helps.
Jim
--
James M. Ehrman Digital Microscopy Facility Mount Allison University 63B York St. Sackville, NB E4L 1G7 CANADA
Simple, clear purpose and principles give rise to complex and intelligent behavior. Complex rules and regulations give rise to simple and stupid behavior. — Dee Hock
On 2/9/2018 5:09 AM, microscopy.listserver-at-gmail.com wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: wschneider-at-wisc.edu } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both wschneider-at-wisc.edu as well as the } Microscopy Listserver } --------------------------------------------------------------------------- } } Email: wschneider-at-wisc.edu Name: Bil Schneider } Organization: UW Madison } Title-Subject: [Filtered] Tungsten filaments } } Message: We use tungsten filaments in our Hitachi S3400N VPSEM. Does } anyone have any suggestions for extending filament life? We try and } operate slightly under saturated except for EBSD or EDS mapping. I’ve } heard of “seasoning” the filament but have never seen an actual protocol } or talked with anybody who’s actually done it. Any suggestions would be } appreciated! } } Login Host: 24.240.36.43 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 7, 53 -- From microscopy.listserver-at-gmail.com Fri Feb 9 03:08:08 2018 } 7, 53 -- Received: from mail-oi0-f51.google.com (mail-oi0-f51.google.com [209.85.218.51]) } 7, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w19988gd004954 } 7, 53 -- for {microscopy-at-microscopy.com} ; Fri, 9 Feb 2018 03:08:08 -0600 } 7, 53 -- Received: by mail-oi0-f51.google.com with SMTP id c189so5483521oib.12 } 7, 53 -- for {microscopy-at-microscopy.com} ; Fri, 09 Feb 2018 00:03:40 -0800 (PST) } 7, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 7, 53 -- d=gmail.com; s=20161025; } 7, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 7, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 7, 53 -- bh=IqkG8nut+Jdti8HQSfY1x1ROkr5cfjQ7/oJEo5aM9XU=; } 7, 53 -- b=EVlvyZdIAsR8eih/9KObYEtcdFgzguDCb55CCzXShQz90Xf1Cldpy6tfwF9m/EkMsC } 7, 53 -- jGjIBNUJfDCfm9KyUQQls/kFzkF0MgUVrmB5wA3QRHQz1dYWNF2EACRS/AWO7H6MWYPt } 7, 53 -- LS9zMx+91CrrwffiZByTchBHBrRD4RUT5efDKZnLMJrClgS6ViC4XB/tZd4nb4/s7LYr } 7, 53 -- U+i+npdk2TjdeHQhxlHrlOFHdJx4I4zU60nSVs6JVMj1KeMLmNtwZA5oM2FIjMk4jDwo } 7, 53 -- lad0JucueoehwzJZCXAJWm6SN+wlKuHXWw8G4BXFjlFIdsuDBXCqWI/IAv2CDGaoqMPz } 7, 53 -- 1tgw== } 7, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 7, 53 -- d=1e100.net; s=20161025; } 7, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 7, 53 -- :user-agent:mime-version:in-reply-to:content-language } 7, 53 -- :content-transfer-encoding; } 7, 53 -- bh=IqkG8nut+Jdti8HQSfY1x1ROkr5cfjQ7/oJEo5aM9XU=; } 7, 53 -- b=fD+cL9JoveCo1gY25/ItNcAhDjdwMxWjC/nN2BCljC4Yxo9qe7djtBIneYz+lcJXwY } 7, 53 -- yoIYyB7+yPrnzqjB+nMOdMpMOS861ExV4iL7giqTyacbPbhtrU5afW+kzRFM+P3QeTZM } 7, 53 -- YCD+u4yABUKD/q8bvjBvuPPoU2CCrPInYbF6m/e3zOp82riqFZOobhIUKQdYa0JHSFuC } 7, 53 -- kllnIeI9ozHP42ETXgWK1wdfxh1wt7GfnM2S4JXLspGNvqx9nNnW/xf+AMihV2dDz3VK } 7, 53 -- sGJVqxhUoiSezS6on2ZWju8Vd3QUZ3hjh6j/BMwYV6rBzng6VcajvMmy5F8A/Otibz9f } 7, 53 -- m6bg== } 7, 53 -- X-Gm-Message-State: APf1xPC5yfDA+vC7r9TgCoRlqVjSLX5y1lF9t1v0/w+RHEovBFYCfUnO } 7, 53 -- fpZxUJ3RcGAfC48i/swFjYdIjA== } 7, 53 -- X-Google-Smtp-Source: AH8x227er67ZL/nArsLNlKydzXDv1ijuT+HuppzwHg+W1JhNLm6181KarZjPxiiFdJveAgRWiYK4MQ== } 7, 53 -- X-Received: by 10.202.229.206 with SMTP id c197mr1135491oih.214.1518163419414; } 7, 53 -- Fri, 09 Feb 2018 00:03:39 -0800 (PST) } 7, 53 -- Received: from Nestors-MacBookAir-Pro-2014-ElCapitan-OSX-1163.local ([175.103.25.178]) } 7, 53 -- by smtp.googlemail.com with ESMTPSA id q57sm918364otq.25.2018.02.09.00.03.37 } 7, 53 -- for {microscopy-at-microscopy.com} } 7, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 7, 53 -- Fri, 09 Feb 2018 00:03:38 -0800 (PST) } 7, 53 -- Subject: viaWWW:Tungsten filaments } 7, 53 -- References: {201802090157.w191vncu007124-at-microscopy.com} } 7, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 7, 53 -- From: Microscopy Listserver {microscopy.listserver-at-gmail.com} } 7, 53 -- X-Forwarded-Message-Id: {201802090157.w191vncu007124-at-microscopy.com} } 7, 53 -- Message-ID: {92f37fee-f8ae-caf6-f04a-6a84f683918c-at-gmail.com} } 7, 53 -- Date: Fri, 9 Feb 2018 18:33:38 +1030 } 7, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) } 7, 53 -- Gecko/20100101 Thunderbird/52.6.0 } 7, 53 -- MIME-Version: 1.0 } 7, 53 -- In-Reply-To: {201802090157.w191vncu007124-at-microscopy.com} } 7, 53 -- Content-Type: text/plain; charset=UTF-8; format=flowed } 7, 53 -- Content-Language: en-US } 7, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
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Email: okneill-at-umich.edu Name: Owen Neill
Organization: University of Michigan
Title-Subject: [Filtered] Symposium P06, M&M2018
Message: Dear colleagues,
Apologies for the cross-postings. The deadline for abstract submission for the Microscopy & Microanalysis 2018 Annual Meeting (Baltimore, MD, 5-9 August, 2018) is February 15th, just a week away. We hope you will consider submitting an abstract to:
Symposium P06, Applications of Integrated Electron Probe Microscopy and Microanalysis in Characterizing Natural and Synthetic Materials.
A full description of this session is included below we are looking for submissions dealing with the synthesis of different microbeam techniques for evaluating composition, bonding, valence, and density-of-state in natural and synthetic materials. Papers may be submitted via the M&M2018 website, which is linked here: https://www.microscopy.org/MandM/2018/index.cfm
We are also pleased to announce the invited speakers for this symposium: Emma Bullock, Carnegie Institution Paul Edwards, Strathclyde University Raynald Gauvin, McGill University Peter Statham, Oxford Instruments Ed Vicenzi, Museum Conservation Institute
We are very excited to hear from these experts in microanalysis, and we look forward to hearing about your work as well. See you in Baltimore!
The organizers of Symposium P06: Kat Crispin, Pennsylvania State University Colin MacRae, CSIRO Mineral Resources Owen Neill, University of Michigan
Symposium Description: Electron beam instruments have been merging with other techniques to extract information such as bonding, valence, and density-of-state, which, combined with elemental analysis, offers new insights into the structure and chemistry of materials. Researchers now have access to an analytical toolbox enabling characterization in a range of controlled environments (liquid and gas) and conditions (elevated temperatures to liquid helium). This symposium showcases synergies of new and emerging techniques, while highlighting recent advances in areas such as optical analysis using RAMAN, cathodoluminescence, soft X-ray spectrometry, and others. Papers covering some or all of these new developments and re-emerging technologies are welcome.
Topics of interest include: Multi-technique, electron-microbeam-based characterizations of natural and synthetic materials. Integration of new and existing technologies to measure material chemistry, structure, boding, valence, etc. Applications of such technologies to natural and synthetic materials.
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Email: mike.marko.em-at-gmail.com Name: MIke Marko
Organization: NY
Title-Subject: [Filtered] Opportunity to learn Cryo-EM and get paid for it!
Message: Opportunity to learn Cryo-EM and get paid for it!
Dr. Rajendra Agrawals group at Wadsworth Center in Albany, NY is looking for an electron microscopy technologist for cryo-EM data collection.
This is an opportunity for someone who knows the basics of TEM operation to learn the hottest technique in biology. You will be using high-end state-of-the-art equipment and software and will be part of an internationally competitive group that has years of experience and consistent grant funding. Our group evolved from the pioneering work -- at Wadsworth -- of Joachim Frank, who shared the 2017 Nobel prize. Other PIs at our 3DEM facility are at the forefront of cryo-EM development, so Albany can be an exciting place to be. Living expenses in Albany are moderate, and our area is an attractive place to live.
If you are interested in this opportunity, please contact Dr. Agrawal at rajendra.agrawal-at-health.ny.gov
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Email: wfschneider-at-wisc.edu Name: Bil Schneider
Organization: UW Madison Geosciences
Title-Subject: [Filtered] Info on Tungsten Filament optimization
Message: Thanks to all who responded to my S3400N VPSEM tungsten filament questions.
We do try to keep users from venting for 5 minutes after turning off the HV. We probably average 50 hours per filament lately, which is what aggravated me a bit because the last box was more like 80 hours. One challenge is that some users change samples multiple times in a session. Then the next user is in variable pressure changing mag and kV, vacuum, etc..... then someone will run an EBSD map overnight at 30 kV, 100 probe current. We ask a lot from the SEM, in general its a workhorse. I did have several suggestions that seem promising: The Hitachi guy called and suggested new filaments shouldnt be turned on till Vacuum has worked for a couple of hours. He said they try and wait more like 15 minutes between HV off and venting. Several responders further emphasized gently saturating the filament over a few hours, keeping it slightly under saturated for general use, and using some gun bias to concentrate the emission area. One respondent made a great point about using a vacuum gauge on the gun and waiting till Vacuum is in the 10 to the minus 5 range. I honestly dont know what the vacuum is when the Hitachi lets you turn on the HV. Adding more spacers to push the grid cap further away from the filament tip was another suggestion. I was under the impression that how ever many spacers that each individual filament came with was the correct distance as measured at the factory to keep emission current appropriate? Otherwise I handle everything with gloves and care when changing filaments and occasionally use metal polish on the grid cap and threaded holder as well as the anode. More often Ill sonicate the grid cap and holder in methanol when the gun is open to change a filament. Thanks again for all the suggestions, Ill repost this in hopes this collection of information might be useful to others with tungsten filament questions. Cheers, Bil Schneider UW Geosciences
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Title-Subject: [Filtered] Cross section on MgO substrates
Message: Hello I need to study the quality of interface of few nanometer thin layer on MgO substrate. I 've prepared cross sections by mechanical polishing followed by ion milling usig the PIPS. I am not satisfied by the quality of HAADF STEM images, especially when I compared those images to HAADF STEM images from cross sections on SrTiO3 prepared by the wedge polishing with the multiprep from ALLIED. Did someone already succeed in performing cross section on MgO using the wedge polishing? I 've tried several time without succes. What is the angle used, the strength, the trick? Thank you for your help
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X-from: Tom Hrn {tomas.hrncir-at-tescan.com} \ Hello All, in my experience there are 3 very simple rules to extend the tungsten filament lifetime:
1. Vent the microscope by nitrogen and always exchange the sample and pump the chamber quickly. This will minimize the contamination of the vacuum chamber by water and speed up the pumping.
2. After getting HV ready signal (when the gun/chamber is pumped), wait several tens of seconds or longer before heating the filament - the filament lifetime depends on the pressure exponentially. Reaching lower 10-3 Pa in the gun is perfect to extend the filament lifetime.
3. Turn off filament heating manually and wait several minutes before venting the chamber. Like that, the filament will cool down properly. Venting the filament when it is hot decreases its lifetime significantly.
By utilizing long waiting time, the filament lifetime around 1000 hours can be obtained easily, if there is no vacuum leak in the gun. So sometimes it is a question whether to wait longer and reach very long lifetime, or wait shorter and exchange filaments more frequently (changing tungsten filament is cheap and easy). For someone and on some machines, exchanging the filament might take several minutes only so it does not make sense to wait several minutes during each sample exchange procedure. For other users/machines, exchanging the filament might be a nightmare so it would be better to wait longer and extend the filament lifetime :-). Have a nice day, Tomas
We’re looking for a compact storage solution to keep membrane boxes under vacuum and/or inert atmosphere. I am considering the containers offered by SPI ( https://www.2spi.com/item/01602-ab/spidry-sample-preservers/) and I’m also aware of Ted Pella’s bell jars, which are a bit too large for our purposes.
Does anyone have recommendations for other containers we can consider? Thanks!
______________________________________ Steven R. Spurgeon, Ph.D. Staff Scientist Energy and Environment Directorate
Pacific Northwest National Laboratory 902 Battelle Boulevard P.O. Box 999 MSIN:P7-25 Richland, WA 99352
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Email: panagiotis.kastritis-at-embl.de Name: Dr Panagiotis Kastritis
Organization: European Molecular Biology Laboratory, Heidelberg Title-Subject: [Filtered] Electron Microscopist position in Halle, Germany
Message: Dear EM community,
I would like to draw your attention to an open position of EM facility manager at the HALOmem institute, located at the Martin Luther University of Halle-Wittenberg (MLU) in Halle, Germany. Our cryoEM facility includes the 300 kV JEOL JEM-3200FSC (equipped with field emission gun, cryo-stage, in-column energy filter and GATAN K2 direct electron detector). Funds are available to acquire another high-end cryo-electron microscope. We are searching for a staff scientist/facility manager who will oversee the operation of the cryoEM facility: assist users with training on the microscopes, perform data collection and ensure timely maintenance of the microscopes. The position is offered until August 2022 and the call closes on the 5th of March. The candidate should have an extensive experience in electron microscope operation, preferably under cryogenic conditions. The HALOmem institute (http://www.halomem.de/) is interested in membrane protein research and houses research groups with strong expertise in protein biochemistry, X-ray crystallography, NMR and proteomics. Interested candidates should apply via e-mail or request further information from Dr. Panagiotis Kastritis (pk-at-halomem.de) -- Official call (in german): http://www.verwaltung.uni-halle.de/dezern3/Ausschr/18_138.pdf English translation: http://www.halomem.de/images/uploads/EM_manager_ad-eng_inofficial.pdf Job details (in english): http://www.halomem.de/images/uploads/EM_manager_ad-eng_details.pdf (http://www.halomem.de/images/uploads/EM_manager_ad-eng_details.pdf) ---
Thank you very much, Kindest Regards,
Panos -- Dr. Panagiotis Kastritis Structural and Computational Biology Unit European Molecular Biology Laboratory Meyerhofstra?e 1, 69117 Heidelberg, Germany
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} We’re looking for a compact storage solution to keep membrane boxes under vacuum and/or inert atmosphere. I am considering the containers offered by SPI ( https://www.2spi.com/item/01602-ab/spidry-sample-preservers/) and I’m also aware of Ted Pella’s bell jars, which are a bit too large for our purposes. } Does anyone have recommendations for other containers we can consider? Thanks!
I have been using this model for storage and transport of SEM samples but also whole TEM grid boxes:
They work really well, up to the point where they start questioning you at the airport security check where you'd be going with that "crystal ashtray" ;)
Guenter
-- Dr. Guenter Resch Nexperion e.U. - Solutions for Electron Microscopy - www.nexperion.net Ziegelhofstrasse 136/1, 1220 Vienna, Austria - Phone +43 664 94 17 210 Registered at Commercial Court Vienna, FN 397677w - VAT Reg. ATU67962234
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if your SEM computer has still control of vacuum / high tension and scan amp, the easiest solution would be to attach an external scan system like the DISS5 from www.pointelectronic.de I am using.
Next possibility would be to exchange the complete electronics to a new one. Pointelectronic does this for some of the Zeiss / FEI / Jeol scopes. There should be an US-based agent also for their hardware.
Best wishes,
Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Am 10.02.18 um 00:21 schrieb oshel1pe-at-cmich.edu: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } *********************************************************************************** } Forwarded from "Ask a Microscopist" } Please remember that the person asking the question is likely } not a member the listserver, and } **any reply should go directly to the poster** } as well as to the list. } Using the "reply" function in your email does *not* send your answer } to the person asking the question. } Please copy their email address from their question. } *********************************************************************************** } } } } } } } Name:karl Hagglund } School:Northwestern University } Grade/Education Level:Graduate } Location:Evanston, IL } US Email:karl.hagglund-at-Northwestern.edu {mailto:karl.hagglund-at-Northwestern.eduSubject} } Subject {mailto:karl.hagglund-at-Northwestern.eduSubject} :Parts Needed Leo/Zeiss 1525 imaging board } Your Question: } Our Leo 1525 has suffered a failure on the imaging board resident on its vintage Windows 98 PC. This is a key system on the microscope, and we have learned that Zeiss no longer has replacements available, and there is not a current computer upgrade path. If there a user out there who might have an old PC is the cupboard from their Vintage Zeiss/Leo SEM, I would be very interested in hearing about it. We are looking for replacement boards for our 20 year old LEO PC. These systems have frame grabbing and scan control boards resident on the system computers, and we are in need of replacement parts. } Thank you, } Karl Hagglund } } } } } } ==============================Original Headers============================== } 10, 59 -- From oshel1pe-at-cmich.edu Fri Feb 9 16:58:07 2018 } 10, 59 -- Received: from NAM01-BN3-obe.outbound.protection.outlook.com (mail-bn3nam01on0113.outbound.protection.outlook.com [104.47.33.113]) } 10, 59 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w19Mw62x009150 } 10, 59 -- for {microscopy-at-microscopy.com} ; Fri, 9 Feb 2018 16:58:07 -0600 } 10, 59 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 10, 59 -- d=CentralMichigan.onmicrosoft.com; s=selector1-cmich-edu; } 10, 59 -- h=From:Date:Subject:Message-ID:Content-Type:MIME-Version; } 10, 59 -- bh=UKlhs70T8N2RchOoD5XEkqH/C+5CkuB8zI2H8YME/7A=; } 10, 59 -- b=OgcR5ZQlZB2cg+Lc6nMDHbX6DkhW7M4aONwtOxdVLewFCgsiaHth+fj0YCWJArD0hez8aScoYPyEYAu/KQoV5ghtez33mhoajIdyA2HS8ebvmJ8L6qqXhifJsk3JV6qes9Q6xcUuKxbS84wscL0olahXsoPYxBx/pYrk9x57rW0= } 10, 59 -- Received: from CY1PR0501MB1676.namprd05.prod.outlook.com (10.163.140.15) by } 10, 59 -- CY1PR0501MB1386.namprd05.prod.outlook.com (10.160.148.140) with Microsoft } 10, 59 -- SMTP Server (version=TLS1_2, } 10, 59 -- cipher=TLS_ECDHE_RSA_WITH_AES_256_CBC_SHA384_P256) id 15.20.485.3; 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==============================Original Headers============================== 10, 30 -- From diller-at-stefan-diller.com Tue Feb 13 04:18:23 2018 10, 30 -- Received: from mailout020.rox.net (mailout020.rox.net [212.63.85.220]) 10, 30 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w1DAINW7013657 10, 30 -- for {microscopy-at-microscopy.com} ; Tue, 13 Feb 2018 04:18:23 -0600 10, 30 -- Received: from localhost ([127.0.0.1]) 10, 30 -- by mailout02.rox.net with esmtp (Exim 4.80) 10, 30 -- (envelope-from {diller-at-stefan-diller.com} ) 10, 30 -- id 1elWf9-000Dpw-4T; Tue, 13 Feb 2018 10:14:07 +0100 10, 30 -- Received: from pd9f9d614.dip0.t-ipconnect.de ([217.249.214.20] helo=mac-pro.local) 10, 30 -- by mailout02.rox.net with esmtpsa (TLSv1.2:DHE-RSA-AES128-SHA:128) 10, 30 -- (Exim 4.80) 10, 30 -- (envelope-from {diller-at-stefan-diller.com} ) 10, 30 -- id 1elWf8-000Dpg-W3; Tue, 13 Feb 2018 10:14:07 +0100 10, 30 -- Subject: Re: [Microscopy] FW: Ask a Microscopist - Imaging board needed for a 10, 30 -- Leo 1525 10, 30 -- To: "Microscopy-at-microscopy.com" {microscopy-at-microscopy.com} , 10, 30 -- karl.hagglund-at-Northwestern.edu 10, 30 -- References: {201802092321.w19NLhWW032075-at-microscopy.com} 10, 30 -- From: stefan diller {diller-at-stefan-diller.com} 10, 30 -- Message-ID: {d476038a-215e-5276-facb-707aed647904-at-stefan-diller.com} 10, 30 -- Date: Tue, 13 Feb 2018 10:14:05 +0100 10, 30 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.13; rv:52.0) 10, 30 -- Gecko/20100101 Thunderbird/52.6.0 10, 30 -- MIME-Version: 1.0 10, 30 -- In-Reply-To: {201802092321.w19NLhWW032075-at-microscopy.com} 10, 30 -- Content-Type: text/plain; charset=windows-1252; format=flowed 10, 30 -- Content-Transfer-Encoding: 7bit 10, 30 -- Content-Language: en-GB 10, 30 -- X-Envelope-From: {diller-at-stefan-diller.com} 10, 30 -- X-Scanned-By: rockenstein AG ==============================End of - Headers==============================
I have just picked up this offering and with 50 years EM experience I fully agree with Tomas' posting. This procedure should be in your s.o.p. for running a scanning electron microscope, any other way is just not good enough!
Steve
Steve Chapman FRMS Protrain for Consultancy and Training in Electron Microscopy +44 (0)7711 606967 web www.emcourses.com
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: 12 February 2018 21:15 To: protrain-at-emcourses.com
X-from: Tom Hrn {tomas.hrncir-at-tescan.com} \ Hello All, in my experience there are 3 very simple rules to extend the tungsten filament lifetime:
1. Vent the microscope by nitrogen and always exchange the sample and pump the chamber quickly. This will minimize the contamination of the vacuum chamber by water and speed up the pumping.
2. After getting HV ready signal (when the gun/chamber is pumped), wait several tens of seconds or longer before heating the filament - the filament lifetime depends on the pressure exponentially. Reaching lower 10-3 Pa in the gun is perfect to extend the filament lifetime.
3. Turn off filament heating manually and wait several minutes before venting the chamber. Like that, the filament will cool down properly. Venting the filament when it is hot decreases its lifetime significantly.
By utilizing long waiting time, the filament lifetime around 1000 hours can be obtained easily, if there is no vacuum leak in the gun. So sometimes it is a question whether to wait longer and reach very long lifetime, or wait shorter and exchange filaments more frequently (changing tungsten filament is cheap and easy). For someone and on some machines, exchanging the filament might take several minutes only so it does not make sense to wait several minutes during each sample exchange procedure. For other users/machines, exchanging the filament might be a nightmare so it would be better to wait longer and extend the filament lifetime :-). Have a nice day, Tomas
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Title-Subject: [Filtered] Career Opportunity - Electron Microscopy Technologist
Message: Research Associate Electron Microscopy Technologist
Cold Spring Harbor Laboratory seeks a highly motivated dedicated individual to work in a state-of-the-art Microscopy Shared Resource. The individual must have extensive practical expertise in biological sample preparation for transmission and scanning electron microscopy of animal tissues and mammalian cell lines. Hands-on knowledge of confocal and widefield fluorescence microscopy would also be a plus.
The candidate will help users design innovative experiments and they will carry out sample preparation and imaging as well as assist in data interpretation.
Excellent verbal and written communication skills, ability to work with multiple users in a supporting role, and ability to work independently and proactively with limited supervision are essential. A Bachelors degree in biology or related discipline is required. One to three years of experience working in a Microscopy Shared Resource is preferred.
How to Apply
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Position Number 01779-R
Applicants should include a resume along with a description of their practical expertise and the names as well as email addresses of 3 references.
Cold Spring Harbor Laboratory is a world-renowned research and educational institution recognized internationally for its excellence in ground-breaking research programs in cancer, neuroscience, plant biology, genomics, and bioinformatics and broad educational mission.
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Email: chrisbrantner-at-gwu.edu Name: Chris Brantner
Organization: George Washington University
Title-Subject: [Filtered] GWNIC to host afternoon of high pressure freezing lectures
Message: Hi all,
The George Washington University Nanofabrication and Imaging Center would like to invite you to an afternoon of high pressure freezing lectures on Monday March 5, 2018. So if you are in Washington DC, just drop around and spend an inspiring afternoon with some local experts in the field of high pressure freezing.
https://nic.gwu.edu/high-pressure-freezing
Chris Brantner
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Email: tina.williams-at-ars.usda.gov Name: Tina Williams
Organization: USDA
Title-Subject: [Filtered] old style FEI camera
Message: Hello,
We have an old style FEI camera film cassette holder that fits an FEI Technai 12. If anyone is interested please contact me offline.
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I want to thank everyone who responded to my TEM question and I regret things are so crazy I can't respond to each person. The information provided was very useful and I plan to remove and clean the Wehneit cup and reinstall. That should, I hope resolve my problems.
Stay safe...........
Frank Karl Microscopist Akron Rubber Development Laboratory 2887 Gilchrist Road Akron, Ohio 44305
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Title-Subject: [Filtered] Job Opening - NASA Johnson Space Center - SEM/EBSD
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Email: rvancamp-at-kettering.edu Name: Rick
Organization: Kettering University
Title-Subject: [Filtered] JEM-2100 Plus Parts Need - Virtually Urgent
Message: Hello Everyone,
I am the microscopist at Kettering University. We own a JEM-2100 Plus TEM. I recently observed that the Wehnhelt and the cathode mounting base the Wehnhelt screws onto have experienced damage to their mating threads. This precludes these parts from being used. I am posting this message to the community in an attempt to identify parts we can obtain from those that have these two parts in addition to the ability to loan them to us on a temporary basis or sell them to us.
The JEM-2100 Plus features a Wehnelt and cathode mount that are common to many Jeol instruments yet, I do not have a complete list. We must consider consulting Jeol to ensure compatibility before any transaction occurs. Note this is the Jeol K-type cathode mount. For example, our TEM uses the Jeol MA113008 (03) tungsten filament, the Denka M3 LKSH 60-degree sharp tip LaB6 cathode, and the APTech mini-Vogel mount 15927 LaB6 cathode. These cathodes are provided as a reference only. I do not know the complete list of LaB6 cathodes that are compatible with our JEM-2100 Plus.
Please contact me if you have additional questions or think you are in a position to help us. Thank you for your consideration.
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Hello, I am teaching a TEM class and I am having trouble finding reliable, readable mappings of the Kikuchi lines for diamond Si. There seems to be a couple of hand-drawn ones floating around, but they are very low-res. I was wondering if anyone had one they could share, or direct me to a place where they can be simulated?
Thank you!
==============================Original Headers============================== 2, 49 -- From klappar.panova-at-gmail.com Wed Feb 14 15:25:19 2018 2, 49 -- Received: from mail-pl0-f48.google.com (mail-pl0-f48.google.com [209.85.160.48]) 2, 49 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w1ELPJOf019904 2, 49 -- for {Microscopy-at-microscopy.com} ; Wed, 14 Feb 2018 15:25:19 -0600 2, 49 -- Received: by mail-pl0-f48.google.com with SMTP id bd10so4321596plb.1 2, 49 -- for {Microscopy-at-microscopy.com} ; Wed, 14 Feb 2018 12:21:09 -0800 (PST) 2, 49 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 2, 49 -- d=gmail.com; s=20161025; 2, 49 -- h=from:content-transfer-encoding:mime-version:subject:message-id:date 2, 49 -- :to; 2, 49 -- bh=89huRmq9vMtZbuC0+cev7qPL+JW8QObtv5fcb/bbnMs=; 2, 49 -- b=YevWMpCF7AEZ+RJbHC8mByfMH4isHHyrRPfivPHQPB/8aLQuQaYw29WZAjh3X3Xl6d 2, 49 -- Vg1uVy58lZjBruLbUIk/WNsm6/FwWIANnyDGRgTIvn3OM3dQlQ2JsYqvgZGhhkK0+FLU 2, 49 -- vpghCzx193e9+kPEpX3dhLhFH3/imyH7Z1bRYBQ2aFDawLV/EMp+074Ro8IY9NbyXEHl 2, 49 -- QHWJczOJFaTuDfeJzs5m49yOCMTbjFJPY61B7uOKapbm2GVp27wMoWsxEdZX4CErUcGA 2, 49 -- T6bSjCpKvWRjgP9bJrrglVhLX5UtqmZxjWuIrMy5gVeOHNO2UHoYOL6gs3yGqZCBB8XU 2, 49 -- FcRQ== 2, 49 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 2, 49 -- d=1e100.net; s=20161025; 2, 49 -- h=x-gm-message-state:from:content-transfer-encoding:mime-version 2, 49 -- :subject:message-id:date:to; 2, 49 -- bh=89huRmq9vMtZbuC0+cev7qPL+JW8QObtv5fcb/bbnMs=; 2, 49 -- b=gitLKQVLNqw8T3XVPs++nmo1DxueqnPSLtSdSN6sWX0g8cTtCZF6jETmHRlVX/QteM 2, 49 -- DhNEH8bKiaci7bpCQoxOqYrk8rJTHRZbD4GAprUxo0eXGnMOjB1t3U82lAdLQA++qOEo 2, 49 -- bG0EZSE91sGltC9nBJ+2sig//5ORmVTxAnOIfICxkESIFKBYYv072+JJrTV+wnqKHoZx 2, 49 -- KxHJy3+AVkEGHCKmHNSZhe2kD/jmg94KcaJxhLPctvDBVCdDjonjpMvwQ8cxUGUyK4aw 2, 49 -- 0yx7s3QDddbuYVDWgRKnb8t4xPuH1m4HdBSPWXnQC+Cp0FiKXolpDV+Fm0i5vllbjhbc 2, 49 -- Deew== 2, 49 -- X-Gm-Message-State: APf1xPDG7Q4bc2BvX/OlTUJXLIuEit4a8XUkzruN6pP80vytwXugE3Kj 2, 49 -- 0+ZCkpBrt5PLT1v29yfKg5xt//2P 2, 49 -- X-Google-Smtp-Source: AH8x224M6n7KSOENkgneFAj6PkmeKw1KEkSqAKGCWNFKvnPvBqE4oRzs2WdVunt8/qEByrkB6j7JJQ== 2, 49 -- X-Received: by 2002:a17:902:8a89:: with SMTP id p9-v6mr189796plo.397.1518639668368; 2, 49 -- Wed, 14 Feb 2018 12:21:08 -0800 (PST) 2, 49 -- Received: from [192.168.11.11] (c-73-70-192-182.hsd1.ca.comcast.net. [73.70.192.182]) 2, 49 -- by smtp.gmail.com with ESMTPSA id c185sm40883137pfb.146.2018.02.14.12.21.06 2, 49 -- for {Microscopy-at-microscopy.com} 2, 49 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 2, 49 -- Wed, 14 Feb 2018 12:21:07 -0800 (PST) 2, 49 -- From: Ouliana Panova {klappar.panova-at-gmail.com} 2, 49 -- Content-Type: text/plain; 2, 49 -- charset=us-ascii 2, 49 -- Mime-Version: 1.0 (Mac OS X Mail 11.2 \(3445.5.20\)) 2, 49 -- Subject: Kikuchi maps 2, 49 -- Message-Id: {D16A7B71-3F9D-4613-9B2A-F41FF8886EDD-at-gmail.com} 2, 49 -- Date: Wed, 14 Feb 2018 12:21:06 -0800 2, 49 -- To: Microscopy-at-microscopy.com 2, 49 -- X-Mailer: Apple Mail (2.3445.5.20) 2, 49 -- Content-Transfer-Encoding: 8bit 2, 49 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id w1ELPJOf019904 ==============================End of - Headers==============================
I recently got a second hand Noran Quest EDX system which I currently can't power on since the detector temperature is too high. I let it cool down for 24 hours with a fully filled up dewar. I believe that the measurement of the "detector temp" is wrong since there is no voltage (0.00 Volts) measured at all. Also, there is no voltage difference between a warm detector and a detector that has cooled down for 24 hours. I am thus looking for technical information including schematics. I suspect that there could be a contact issue or a broken cable.
I started the Noran Quest "sys_status" tool. It gives me the following output:
As you can see, the "detector temp" is 0.00 Volts.
- What are normal voltage levels for the temperature measurements ? (If the LN sensor voltage is 4.28 Volts, how does that translate to °C ? Is there a formula to translate the voltage to temperature? Is 4.28 Volts OK for a filled up dewar ?)
- What voltage should I get for the "detector temp" ? (What voltage should be measured if the detector is warm ? What voltage should be there if the detector is cooled down ?)
- Where is the temperature sensor physically located within the detector ?
- What kind of temperature sensor is it (resistor with X Ohms) ?
- At the detector there is a d-sub connector. What is the pinout of this connector and where is the "detector temp" output ?
Any technical information to get my detector working would be really appreciated.
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Email: lisa.odonovan-at-adelaide.edu.au Name: Lisa O'Donovan
Organization: The University of Adelaide, Adelaide Microscopy
Title-Subject: [Filtered] ancient grain
Message: Hi all, I have been tasked with imaging an ancient grain. It is 1000 year old millet and I have one only! I have done SEM/TEM of grain before but not one so old. I am thinking SEM may be the way to go. The investigators would like to see the internal structure of the grain (if any) and I have no idea whether it will be 'normal', caramelised or powder inside! It must be fixed in order to be released from quarantine so my first question is should I use an aqueous fixative or alcohol? Any other advice would be gratefully accepted!
Cheers Lisa
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Email: btracy-at-eag.com Name: Bryan Tracy
Organization: EAG Laboratories
Title-Subject: [Filtered] cutting the slab?
Message: Hello Everyone,
I know this is a subject on which reasonable people can disagree, but I wanted to ask what has been your experience with cutting the slab to reduce low frequency vibrations?
The floor appears to be moving in the horizontal plane at 1.6Hz (4.4 reduction factor needed) and in the vertical direction at 5HZ. (3.1 reduction factor needed)
much appreciated
bryan
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X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu}
Lisa,
It’s a grain, so largely starch, meaning formaldehyde and glut won’t fix much anyway. If alcohol is good enough to release from quarantine, use 70 or 80% ethanol, then go through to 100% EtOH. Either dry from ethanol or go to tert-butyl alcohol and vacuum sublimate at 20 deg C.
After the 2nd or 3rd 100% EtOH, you could put the grain in liquid nitrogen and hit it with a razor blade, maybe gently crush it. You’ll get a brittle fracture that will expose the starch grains. This part will be particularly fun (or “fun”) if your grain is tiny ...
Phil ------------- Philip Oshel Imaging Facility Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 989 774-3576 office 989 774-7567 lab
Email: lisa.odonovan-at-adelaide.edu.au Name: Lisa O'Donovan
Organization: The University of Adelaide, Adelaide Microscopy
Title-Subject: [Filtered] ancient grain
Message: Hi all, I have been tasked with imaging an ancient grain. It is 1000 year old millet and I have one only! I have done SEM/TEM of grain before but not one so old. I am thinking SEM may be the way to go. The investigators would like to see the internal structure of the grain (if any) and I have no idea whether it will be 'normal', caramelised or powder inside! It must be fixed in order to be released from quarantine so my first question is should I use an aqueous fixative or alcohol? Any other advice would be gratefully accepted!
Do you know if the vibration is resulting form sources inside the building or the from the general area outside? EAG is in between a number of freeways there in Sunnyvale and the 101 isn't far away so if it is the underlying ground below slab, you might make it worse cutting the slab. I'm sure some of the experts on here have experience doing this and will advise.
You might also check with Vibration Engineering Consultants - www.vibeng.com {http://www.vibeng.com} - in Pacifica, CA. This is their specialty.
I would also be remiss if I didn't suggest that you consider a Vibration Isolation Base Platform. The active type we offer suppresses vibration starting at 0.7 Hz and has 90% suppression by 2Hz. I would avoid a passive type isolator as most of them have a resonant frequency right around 2 to 4 Hz which would make things even worse for the vibrations you have. Likewise if you decide to cut the slab, careful if you use an elastomeric sealant and check it's resonant frequency.
Have a look here at our line of Vibration Isolation Platforms for SEM/TEM/FIB: http://www.elementpi.com/active-vibration-isolation-platform-products/
Good Luck,
Mike Toalson element Pi, LLC 833-314-1593
On Fri, Feb 16, 2018 at 2:15 AM, {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote:
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I know this is a subject on which reasonable people can disagree, but I wanted to ask what has been your experience with cutting the slab to reduce low frequency vibrations?
The floor appears to be moving in the horizontal plane at 1.6Hz (4.4 reduction factor needed) and in the vertical direction at 5HZ. (3.1 reduction factor needed)
much appreciated
bryan
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See below for a request from a paleobiologist here at the National Museum of Natural History. If you have a modern image of collagen fibrils you are willing to share in some manner he would be interested in hearing from you directly. His contact is GreenwaltD-at-si.edu.
Thanks,
Scott Whittaker Lab Manager Imaging P.O. Box 37012 MRC-104 Room W150 Washington, DC 20013-7012 w 202.633.0891 mailto:whittaks-at-si.edu
SMITHSONIAN INSTITUTION NATIONAL MUSEUM OF NATURAL HISTORY https://www.facebook.com/nmnh.fanpage/ | https://twitter.com/NMNH | https://www.instagram.com/smithsoniannmnh/
X-from: "Greenwalt, Dale" {GreenwaltD-at-si.edu}
Hi Lisa,
Fixation in 95-100% methanol or ethanol may be better for your grain - there will be less tissue swelling. The dry grain will contain only about 8-10% water, so going into 100% solvent will be fine. A little water (i.e. 95% solvent) may help penetration. Methanol will penetrate a little better, but it will take some time for any solvent to get deep into a dry grain.
Another option after drying would be high-resolution (+/- phase contrast) x-ray CT - it would quickly show you if the grain had any contents, and give you an idea of what they are without breaking the seed. There are at least a couple of labs I know of in Australia that do this, one of them is here in Canberra at the ANU - https://ctlab.anu.edu.au/, and since your seed will be dead after going through solvent, you don't have to worry about the high kV x-rays killing the tissue. Millet seed is pretty small, so you'd get good resolution of the innards.
cheers, Rosemary
Dr Rosemary White CSIRO Black Mountain GPO Box 1700 Canberra, ACT 2601 Australia Adjunct Prof, EH Graham Centre, CSU & Research School of Biology, ANU
T 61 2 6246 5475 E rosemary.white-at-csiro.au
On 17/2/18, 7:29 am, "microscopy.listserver-at-gmail.com" {microscopy.listserver-at-gmail.com} wrote:
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X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu}
Lisa,
It’s a grain, so largely starch, meaning formaldehyde and glut won’t fix much anyway. If alcohol is good enough to release from quarantine, use 70 or 80% ethanol, then go through to 100% EtOH. Either dry from ethanol or go to tert-butyl alcohol and vacuum sublimate at 20 deg C.
After the 2nd or 3rd 100% EtOH, you could put the grain in liquid nitrogen and hit it with a razor blade, maybe gently crush it. You’ll get a brittle fracture that will expose the starch grains. This part will be particularly fun (or “fun”) if your grain is tiny ...
Phil ------------- Philip Oshel Imaging Facility Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 989 774-3576 office 989 774-7567 lab
Email: lisa.odonovan-at-adelaide.edu.au Name: Lisa O'Donovan
Organization: The University of Adelaide, Adelaide Microscopy
Title-Subject: [Filtered] ancient grain
Message: Hi all, I have been tasked with imaging an ancient grain. It is 1000 year old millet and I have one only! I have done SEM/TEM of grain before but not one so old. I am thinking SEM may be the way to go. The investigators would like to see the internal structure of the grain (if any) and I have no idea whether it will be 'normal', caramelised or powder inside! It must be fixed in order to be released from quarantine so my first question is should I use an aqueous fixative or alcohol? Any other advice would be gratefully accepted!
X-from: Henk Colijn {colijn.1-at-osu.edu} Reply-To: Henk Colijn {colijn.1-at-osu.edu} To: microscopy.listserver-at-gmail.com
Hi Bryan,
Those are very low frequencies. They should be in a range that an active compensation system should be able to handle it.
Another thing to consider is the resonant frequency of the slab (even though it is damped by the ground). The more mass in the slab, the lower its resonant frequency. The install guides for our microscopes show that the microscope is much more sensitive at very low frequencies. The allowable vibration at 10Hz is approx 10x lower than at 20Hz. Since too much mass can push the slab resonance down into the range where the microscope is more sensitive you may want to estimate the resonant frequency of your current slab and then consider whether to slice it or not. It would also be useful to measure the ground vibration away from the instrument to get an idea of the driving frequencies.
Good luck! Henk
--------------------
Hendrik O. Colijn Center for Electron Microscopy and AnalysiS The Ohio State University 1305 Kinnear Rd, Suite 100, Columbus, OH 43212
colijn.1-at-osu.edu 614/643-3458 cemas.osu.edu
"Time is that quality of nature which keeps things from happening all at once." (Ray Cummings - 1922) Lately it doesn't seem to be working.
------ Original Message ------ X-from: microscopy.listserver-at-gmail.com To: colijn.1-at-osu.edu Sent: 2/16/2018 4:45:19 AM
X-from: Lisa Anne O'Donovan {lisa.odonovan-at-adelaide.edu.au}
Thanks Phil. I’ve imaged grain before and I’ve found the best result after soaking it for 48 hrs in water and then cutting it into pieces before fixing and dehydration. Unfortunately I have no idea if soaking this grain might have a more detrimental effect. Thanks for your advice
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On 17 Feb 2018, at 6:14 am, "microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} " {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu {mailto:oshel1pe-at-cmich.edu} } } } Lisa, } } It’s a grain, so largely starch, meaning formaldehyde and glut won’t fix } much anyway. If alcohol is good enough to release from quarantine, use } 70 or 80% ethanol, then go through to 100% EtOH. } Either dry from ethanol or go to tert-butyl alcohol and vacuum sublimate } at 20 deg C. } } After the 2nd or 3rd 100% EtOH, you could put the grain in liquid } nitrogen and hit it with a razor blade, maybe gently crush it. You’ll } get a brittle fracture that will expose the starch grains. } This part will be particularly fun (or “fun”) if your grain is tiny ... } } Phil } ------------- } Philip Oshel } Imaging Facility Director } Biology Department } 1304 Biosciences } 1455 Calumet Ct. } Central Michigan University } Mt. Pleasant, MI 48859 } 989 774-3576 office } 989 774-7567 lab } } } } } } Email: lisa.odonovan-at-adelaide.edu.au {mailto:lisa.odonovan-at-adelaide.edu.au} Name: Lisa O'Donovan } } Organization: The University of Adelaide, Adelaide Microscopy } } Title-Subject: [Filtered] ancient grain } } Message: Hi all, } I have been tasked with imaging an ancient grain. It is 1000 year old } millet and I have one only! I have done SEM/TEM of grain before but not } one so old. I am thinking SEM may be the way to go. The investigators } would like to see the internal structure of the grain (if any) and I } have no idea whether it will be 'normal', caramelised or powder inside! } It must be fixed in order to be released from quarantine so my first } question is should I use an aqueous fixative or alcohol? } Any other advice would be gratefully accepted! } } Cheers } Lisa } } } } ==============================Original Headers============================== } 16, 53 -- From microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} Fri Feb 16 } 14:19:28 2018 } 16, 53 -- Received: from mail-pl0-f46.google.com {http://mail-pl0-f46.google.com} } (mail-pl0-f46.google.com {http://mail-pl0-f46.google.com} [209.85.160.46]) } 16, 53 -- by microscopy.com {http://microscopy.com} (8.12.11.20060308/8.12.8) with ESMTP id } w1GKJS9B005923 } 16, 53 -- for {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } ; Fri, 16 Feb 2018 } 14:19:28 -0600 } 16, 53 -- Received: by mail-pl0-f46.google.com {http://mail-pl0-f46.google.com} with SMTP id } p5so2166846plo.12 } 16, 53 -- for {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } ; Fri, 16 Feb } 2018 11:15:24 -0800 (PST) } 16, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 16, 53 -- d=gmail.com {http://gmail.com} ; s=20161025; } 16, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 16, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 16, 53 -- bh=NBGWJ+Ii7A39wsCa0/ly9zp+iQw8nF2l9NC7qHai2To=; } 16, 53 -- b=GE5+xXLFrelnACEZ+qb9LrabEgUCAn6Lq2squbWTpQikdFp75iK6bIxh2zFnC3KhiR } 16, 53 -- +zjgqcMwRwH+GTq2JSAgvTFfMB55JJgUzYYk7dd8i1L4pBeh38LWDDvF/4zFBt95T1UT } 16, 53 -- 8WAPsoPlzS3aoVcpxiCJ+JClDJCZXyiE0alPAWG4ZnWHyttpCgqegVibj4GjOsnbtDSq } 16, 53 -- yw7JMRvrh79fb+vXqwS0HhRoH/kzVFPiW6ar9kkagZ26AZ7QLDQBrmLm7AAsUh8HfcF6 } 16, 53 -- s+T91z7j8+6LXqNUegeYgPD8N30jfYPaBj36TCyY7mLD+uwNqARcwvcDpO5toDesl/6i } 16, 53 -- /NmA== } 16, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 16, 53 -- d=1e100.net {http://1e100.net} ; s=20161025; } 16, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 16, 53 -- :user-agent:mime-version:in-reply-to:content-language } 16, 53 -- :content-transfer-encoding; } 16, 53 -- bh=NBGWJ+Ii7A39wsCa0/ly9zp+iQw8nF2l9NC7qHai2To=; } 16, 53 -- b=QdtuGp/nzDKOePj1apmNn88W2CmkUDhmqMhywXO7YH/DuCNMx3db1YHX2Urhqjylb/ } 16, 53 -- LPJYbLPHmaHc4+6erBsfifIVqKUMHEjzbU1e9dJ0ilSliShV6btDfGvqvSvl/ToZTfFn } 16, 53 -- DIJQC3eId0NYkyMR97fUoPoLdRvGmARecoAZaYmBsXwGDRLe0Vf3fqCRfQxYlsMAoWCH } 16, 53 -- rj3sULHYi2psR79LQsIPExm2Ej8vSkxI7WXRkGis5cG/GfWxJSIc8bYAOg2VC1MB+DNN } 16, 53 -- gb9HwnikuHtmO7xae337nSnI9bzeA/cn3uuSznrPP3TMu2fQ4loQYuntT98yCZnREGHG } 16, 53 -- Gu4A== } 16, 53 -- X-Gm-Message-State: APf1xPBfFpAudrNxqfQxQRI9reKSggIkGQjJWygh9oB9SNA/mNBcECwm } 16, 53 -- /VgMY+RDCuEltUcH5bksSPdvRQ== } 16, 53 -- X-Google-Smtp-Source: } AH8x227HuGE720DzgnhClPqS9EKAAiKPspMA1UrZJhOFXu32me+/xf87MQiz5g5QIyjCNv44s/yjbw== } 16, 53 -- X-Received: by 2002:a17:902:34e:: with SMTP id 72-v6mr6775268pld.277.1518808523341; } 16, 53 -- Fri, 16 Feb 2018 11:15:23 -0800 (PST) } 16, 53 -- Received: from NestorsnOSX1163.lan (220-244-30-118.static.tpgi.com.au } {http://220-244-30-118.static.tpgi.com.au} . 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I am considering replacing an older critical point dryer for a multi-user microscopy lab that serves faculty and undergraduate student users for research projects, and trains undergraduates via courses. After training, users carry out their own specimen preps.
Two models Ive come across are the Leica EMCPD300 and the Tousimis Autosamdri-931.
I would appreciate any comments from folks who have experience with either of these, as well as suggestions about other models I may have overlooked but should consider.
Feel free to respond directly to me if you would like at jan.factor-at-purchase.edu {mailto:jan.factor-at-purchase.edu} .
/_*Purchase College ranked in the Top 10 public liberal arts colleges nationally* {http://colleges.usnews.rankingsandreviews.com/best-colleges/rankings/national-liberal-arts-colleges/top-public} _/ *Please consider the environment before printing this e-mail*
To: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com}
We have the Tousimis 815B & 915B. We process a lot of samples of varying sizes (some quite large) & they do an excellent job & are easy/straightforward to use. We have an advantage since we're pretty much next door to the company, but they are very helpful. I haven't used the EMS.
Deborah
Sent from my iPhone
} On Feb 18, 2018, at 11:16 AM, "microscopy.listserver-at-gmail.com" {microscopy.listserver-at-gmail.com} wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: Factor, Jan {Jan.Factor-at-purchase.edu} } } } } Dear List: } } I am considering replacing an older critical point dryer for a multi-user microscopy lab that serves } faculty and undergraduate student users for research projects, and trains undergraduates via } courses. After training, users carry out their own specimen preps. } } Two models Ive come across are the Leica EMCPD300 and the Tousimis Autosamdri-931. } } I would appreciate any comments from folks who have experience with either of these, as well as } suggestions about other models I may have overlooked but should consider. } } Feel free to respond directly to me if you would like at jan.factor-at-purchase.edu } {mailto:jan.factor-at-purchase.edu} . } } --Many thanks, *Jan * } } Jan Robert Factor, Ph.D. } } Professor of Biology } } Purchase College, State University of New York } } Purchase, NY 10577 } } Office: 2016 Natural Science Bldg., 914-251-6659 } } Office Hours (Spring 2018): } } Tues., 11:00-12:00, and Thurs., 1:30-2:30 } } jan.factor-at-purchase.edu {mailto:jan.factor-at-purchase.edu} } } /_*Purchase College ranked in the Top 10 public liberal arts colleges nationally* } {http://colleges.usnews.rankingsandreviews.com/best-colleges/rankings/national-liberal-arts-colleges/top-public} _/ } *Please consider the environment before printing this e-mail* } } } ==============================Original Headers============================== } 19, 53 -- From microscopy.listserver-at-gmail.com Sun Feb 18 10:53:43 2018 } 19, 53 -- Received: from mail-it0-f43.google.com (mail-it0-f43.google.com [209.85.214.43]) } 19, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w1IGrhv0021068 } 19, 53 -- for {microscopy-at-microscopy.com} ; Sun, 18 Feb 2018 10:53:43 -0600 } 19, 53 -- Received: by mail-it0-f43.google.com with SMTP id o13so6661083ito.2 } 19, 53 -- for {microscopy-at-microscopy.com} ; Sun, 18 Feb 2018 07:49:45 -0800 (PST) } 19, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 19, 53 -- d=gmail.com; s=20161025; } 19, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 19, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 19, 53 -- bh=se/2HzDj1HUWS5xYRiWI93RZvYwNKqpezKNC3pJ1kYY=; } 19, 53 -- b=LxEpN93LCGD0YU3GetB+jq48DFBz+9v+Z9kV3gp6GnrxsVuJJVHkSQsMAP6i7shQOc } 19, 53 -- YzBkw24axX4c9TI/3Koq0EyrAtnXAoczlONbWMUyDDLes95j0MdbKzFtReRtpKZv7iG1 } 19, 53 -- G5kIR0BcScrU0MvxbTnMKIuglZVpu4Bv6uM2mONKYQWVXOZyF2BmUGywic9kwjFnl/Wv } 19, 53 -- CAdcFraHh+1KaYvY+cDw11WzRP+cDkAbredNwx9Hz3vLw+FUl6H1PdYZmYl9TIpw/q7t } 19, 53 -- MyrScHJD/4ulFNg937MKA5eQ70ghzVmCCVB1PnpReBQ9yjBC0yCCaCmMF5T15A15OzZx } 19, 53 -- DpSg== } 19, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 19, 53 -- d=1e100.net; s=20161025; } 19, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 19, 53 -- :user-agent:mime-version:in-reply-to:content-language } 19, 53 -- :content-transfer-encoding; } 19, 53 -- bh=se/2HzDj1HUWS5xYRiWI93RZvYwNKqpezKNC3pJ1kYY=; } 19, 53 -- b=KnRgyqB37C0aSVFASW+g4cybGgLsXbmheoNnDRXmgMsbue+g3zmcN9JzmqgpJEevus } 19, 53 -- hSB4nG/mfQJXOol3WO8ddF7X0QB7ZGuiVsstomuQLlKL7PO4oHY2zX4hFcL53KL7Lzyt } 19, 53 -- ERCPnJka5wbekD8IO5fHnzeo08ZO+c7hMrNIJ9dDcfFX+mGqEfRyWVyy4FWOGgRf374L } 19, 53 -- jWeq4GhJbDDdlba4Hfslw4hofe240DuDLrwKqXN0RiECclVkfBar0OBcV4JERep5edd2 } 19, 53 -- izMWLhSr9SgxWKnbAuuv3WqnXt2pcqb4mIb8NAKC1a2f8J8dnsl7W3/5jVF4oBS0FWs4 } 19, 53 -- ++2Q== } 19, 53 -- X-Gm-Message-State: APf1xPC45Onm+IuNvfy15EGfclpWyyhmAsGDeCbBSiYolidDCXUL7KOz } 19, 53 -- EU/Tki43CNruzcogrf+W/WUz3iwp } 19, 53 -- X-Google-Smtp-Source: AH8x226QMHZW/VYa84ITElkJvhANGmaV8diteKaAVurUg7n/r2kSuDOjf5H/g3i+09GX3OwcFQqMiQ== } 19, 53 -- X-Received: by 10.36.188.196 with SMTP id n187mr16678372ite.27.1518968984734; } 19, 53 -- Sun, 18 Feb 2018 07:49:44 -0800 (PST) } 19, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:9915:6b6a:bf8d:aa6e]) } 19, 53 -- by smtp.googlemail.com with ESMTPSA id s24sm6136616ioa.34.2018.02.18.07.49.44 } 19, 53 -- for {microscopy-at-microscopy.com} } 19, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 19, 53 -- Sun, 18 Feb 2018 07:49:44 -0800 (PST) } 19, 53 -- Subject: Fwd: Critical point dryer suggestions } 19, 53 -- References: {cf1e8ba6aa794cca959debc5b98c33ef-at-VSMAILMB02.purchase.edu} } 19, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 19, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 19, 53 -- X-Forwarded-Message-Id: {cf1e8ba6aa794cca959debc5b98c33ef-at-VSMAILMB02.purchase.edu} } 19, 53 -- Message-ID: {ffb0d64a-5249-79ae-6249-132258e20caa-at-gmail.com} } 19, 53 -- Date: Sun, 18 Feb 2018 09:49:43 -0600 } 19, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) } 19, 53 -- Gecko/20100101 Thunderbird/52.5.2 } 19, 53 -- MIME-Version: 1.0 } 19, 53 -- In-Reply-To: {cf1e8ba6aa794cca959debc5b98c33ef-at-VSMAILMB02.purchase.edu} } 19, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 19, 53 -- Content-Language: en-US } 19, 53 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers==============================
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X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu} To: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com}
Lisa,
Good luck. I like Rosemary’s suggestion — mine about liquid nitrogen needs decent sized seeds. I’ve used it for corn and barley, but if you do have millet … oof.
Phil ------------- Philip Oshel Imaging Facility Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 989 774-3576 office 989 774-7567 lab
X-from: Lisa Anne O'Donovan {lisa.odonovan-at-adelaide.edu.au}
Thanks Phil. I’ve imaged grain before and I’ve found the best result after soaking it for 48 hrs in water and then cutting it into pieces before fixing and dehydration. Unfortunately I have no idea if soaking this grain might have a more detrimental effect. Thanks for your advice
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On 17 Feb 2018, at 6:14 am, "microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} " {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu {mailto:oshel1pe-at-cmich.edu} } } } Lisa, } } It’s a grain, so largely starch, meaning formaldehyde and glut won’t fix } much anyway. If alcohol is good enough to release from quarantine, use } 70 or 80% ethanol, then go through to 100% EtOH. } Either dry from ethanol or go to tert-butyl alcohol and vacuum sublimate } at 20 deg C. } } After the 2nd or 3rd 100% EtOH, you could put the grain in liquid } nitrogen and hit it with a razor blade, maybe gently crush it. You’ll } get a brittle fracture that will expose the starch grains. } This part will be particularly fun (or “fun”) if your grain is tiny ... } } Phil } ------------- } Philip Oshel } Imaging Facility Director } Biology Department } 1304 Biosciences } 1455 Calumet Ct. } Central Michigan University } Mt. Pleasant, MI 48859 } 989 774-3576 office } 989 774-7567 lab } } } } } } Email: lisa.odonovan-at-adelaide.edu.au {mailto:lisa.odonovan-at-adelaide.edu.au} Name: Lisa O'Donovan } } Organization: The University of Adelaide, Adelaide Microscopy } } Title-Subject: [Filtered] ancient grain } } Message: Hi all, } I have been tasked with imaging an ancient grain. It is 1000 year old } millet and I have one only! I have done SEM/TEM of grain before but not } one so old. I am thinking SEM may be the way to go. The investigators } would like to see the internal structure of the grain (if any) and I } have no idea whether it will be 'normal', caramelised or powder inside! } It must be fixed in order to be released from quarantine so my first } question is should I use an aqueous fixative or alcohol? } Any other advice would be gratefully accepted! } } Cheers } Lisa } } } } ==============================Original Headers============================== } 16, 53 -- From microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} Fri Feb 16 } 14:19:28 2018 } 16, 53 -- Received: from mail-pl0-f46.google.com {http://mail-pl0-f46.google.com} } (mail-pl0-f46.google.com {http://mail-pl0-f46.google.com} [209.85.160.46]) } 16, 53 -- by microscopy.com {http://microscopy.com} (8.12.11.20060308/8.12.8) with ESMTP id } w1GKJS9B005923 } 16, 53 -- for {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } ; Fri, 16 Feb 2018 } 14:19:28 -0600 } 16, 53 -- Received: by mail-pl0-f46.google.com {http://mail-pl0-f46.google.com} with SMTP id } p5so2166846plo.12 } 16, 53 -- for {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } ; Fri, 16 Feb } 2018 11:15:24 -0800 (PST) } 16, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 16, 53 -- d=gmail.com {http://gmail.com} ; s=20161025; } 16, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 16, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 16, 53 -- bh=NBGWJ+Ii7A39wsCa0/ly9zp+iQw8nF2l9NC7qHai2To=; } 16, 53 -- b=GE5+xXLFrelnACEZ+qb9LrabEgUCAn6Lq2squbWTpQikdFp75iK6bIxh2zFnC3KhiR } 16, 53 -- +zjgqcMwRwH+GTq2JSAgvTFfMB55JJgUzYYk7dd8i1L4pBeh38LWDDvF/4zFBt95T1UT } 16, 53 -- 8WAPsoPlzS3aoVcpxiCJ+JClDJCZXyiE0alPAWG4ZnWHyttpCgqegVibj4GjOsnbtDSq } 16, 53 -- yw7JMRvrh79fb+vXqwS0HhRoH/kzVFPiW6ar9kkagZ26AZ7QLDQBrmLm7AAsUh8HfcF6 } 16, 53 -- s+T91z7j8+6LXqNUegeYgPD8N30jfYPaBj36TCyY7mLD+uwNqARcwvcDpO5toDesl/6i } 16, 53 -- /NmA== } 16, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 16, 53 -- d=1e100.net {http://1e100.net} ; s=20161025; } 16, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 16, 53 -- :user-agent:mime-version:in-reply-to:content-language } 16, 53 -- :content-transfer-encoding; } 16, 53 -- bh=NBGWJ+Ii7A39wsCa0/ly9zp+iQw8nF2l9NC7qHai2To=; } 16, 53 -- b=QdtuGp/nzDKOePj1apmNn88W2CmkUDhmqMhywXO7YH/DuCNMx3db1YHX2Urhqjylb/ } 16, 53 -- LPJYbLPHmaHc4+6erBsfifIVqKUMHEjzbU1e9dJ0ilSliShV6btDfGvqvSvl/ToZTfFn } 16, 53 -- DIJQC3eId0NYkyMR97fUoPoLdRvGmARecoAZaYmBsXwGDRLe0Vf3fqCRfQxYlsMAoWCH } 16, 53 -- rj3sULHYi2psR79LQsIPExm2Ej8vSkxI7WXRkGis5cG/GfWxJSIc8bYAOg2VC1MB+DNN } 16, 53 -- gb9HwnikuHtmO7xae337nSnI9bzeA/cn3uuSznrPP3TMu2fQ4loQYuntT98yCZnREGHG } 16, 53 -- Gu4A== } 16, 53 -- X-Gm-Message-State: APf1xPBfFpAudrNxqfQxQRI9reKSggIkGQjJWygh9oB9SNA/mNBcECwm } 16, 53 -- /VgMY+RDCuEltUcH5bksSPdvRQ== } 16, 53 -- X-Google-Smtp-Source: } AH8x227HuGE720DzgnhClPqS9EKAAiKPspMA1UrZJhOFXu32me+/xf87MQiz5g5QIyjCNv44s/yjbw== } 16, 53 -- X-Received: by 2002:a17:902:34e:: with SMTP id 72-v6mr6775268pld.277.1518808523341; } 16, 53 -- Fri, 16 Feb 2018 11:15:23 -0800 (PST) } 16, 53 -- Received: from NestorsnOSX1163.lan (220-244-30-118.static.tpgi.com.au } {http://220-244-30-118.static.tpgi.com.au} . 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22, 53 -- Sun, 18 Feb 2018 07:49:12 -0800 (PST) 22, 53 -- Subject: Fwd: Re: [Microscopy] viaWWW: ancient grain imaging help needed 22, 53 -- References: {103037D3-11ED-4A61-9E2A-62575DC02E90-at-adelaide.edu.au} 22, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 22, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} 22, 53 -- X-Forwarded-Message-Id: {103037D3-11ED-4A61-9E2A-62575DC02E90-at-adelaide.edu.au} 22, 53 -- Message-ID: {aec0a42b-72ad-090e-68aa-cc90c22a2cce-at-gmail.com} 22, 53 -- Date: Sun, 18 Feb 2018 09:49:12 -0600 22, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) 22, 53 -- Gecko/20100101 Thunderbird/52.5.2 22, 53 -- MIME-Version: 1.0 22, 53 -- In-Reply-To: {103037D3-11ED-4A61-9E2A-62575DC02E90-at-adelaide.edu.au} 22, 53 -- Content-Type: text/plain; charset=utf-8; format=flowed 22, 53 -- Content-Language: en-US 22, 53 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu} To: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com}
Jan,
Tousimis is good, I’ve used those a lot. Not the Autosamdri, but the previous model. Have you considered the Polaron “bomb”? The larger sample chamber (in the regular size) is very handy. SPI sells this as a rebranded product. I use it in a multi-user facility that teaches undergrads and grad students.
Phil ------------- Philip Oshel Imaging Facility Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 989 774-3576 office 989 774-7567 lab
I am considering replacing an older critical point dryer for a multi-user microscopy lab that serves faculty and undergraduate student users for research projects, and trains undergraduates via courses. After training, users carry out their own specimen preps.
Two models I’ve come across are the Leica EMCPD300 and the Tousimis Autosamdri-931.
I would appreciate any comments from folks who have experience with either of these, as well as suggestions about other models I may have overlooked but should consider.
Feel free to respond directly to me if you would like at jan.factor-at-purchase.edu {mailto:jan.factor-at-purchase.edu} .
/_*Purchase College ranked in the Top 10 public liberal arts colleges nationally* {http://colleges.usnews.rankingsandreviews.com/best-colleges/rankings/national-liberal-arts-colleges/top-public} _/ *Please consider the environment before printing this e-mail*
From trojlita384otoj-at-gmail.com Mon Feb 19 10:46:40 2018 Return-Path: {trojlita384otoj-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [222.139.205.243] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id w1JGkcuX017428 for {microscopylistserverarchive6-at-microscopy.com} ; Mon, 19 Feb 2018 10:46:39 -0600 Received: from mail.webhostings4u.com [201.236.207.69] by mts.locks.grgtween.net with NNFMP; Mon, 19 Feb 2018 12:26:00 -0300 Received: from group21.345mail.com ([Mon, 19 Feb 2018 12:09:47 -0300]) by mmx09.tilkbans.com with QMQP; Mon, 19 Feb 2018 12:09:47 -0300 Received: from asx121.turbo-inline.com ([Mon, 19 Feb 2018 11:56:49 -0300]) by relay.2yahoo.com with ESMTP; Mon, 19 Feb 2018 11:56:49 -0300 Message-ID: {FB09E1E6.D2B09369-at-gmail.com}
Hi Everyone, I have been asked if our vacuum evaporator will put down tin to 1 micron thickness.
We have a Cressington unit so its not the principle it’s the practice. Does anyone have experience with tin or a thickness of 1 micron or both!
Chris
Christopher J. Gilpin Ph.D. Director, Life Science Microscopy Facility Campus-wide Coordinator for Electron Microscopy Purdue University Whistler Hall of Agriculture Research, Room S052 170 S. University St West Lafayette, IN 47907 765-494-7750 gilpin-at-purdue.edu lsmf-at-purdue.edu reaches everyone in the facility. http://ag.purdue.edu/arp/Microscopy/Pages/default.aspx
==============================Original Headers============================== 8, 40 -- From gilpin-at-purdue.edu Mon Feb 19 16:15:22 2018 8, 40 -- Received: from xppmailspam05.itap.purdue.edu (xppmailspam05.itap.purdue.edu [128.210.5.16]) 8, 40 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w1JMFMn2013820 8, 40 -- for {microscopy-at-microscopy.com} ; Mon, 19 Feb 2018 16:15:22 -0600 8, 40 -- X-Cloudmark-SP-Filtered: true 8, 40 -- X-Cloudmark-SP-Result: =?us-ascii?q?v=3D2=2E1_cv=3DU7Iydbfu_c=3D1_sm=3D2_tr?= 8, 40 -- =?us-ascii?q?=3D0_a=3DdSX5codZc0cA=3A10_a=3DIkcTkHD0fZMA=3A10?= 8, 40 -- =?us-ascii?q?_a=3Dx7bEGLp0ZPQA=3A10_a=3DxqWC=5FBr6kY4A=3A10_a=3DOp4juWPpsa0?= 8, 40 -- =?us-ascii?q?A=3A10_a=3D35Thz0BbAAAA=3A8?= 8, 40 -- =?us-ascii?q?_a=3D99ovruB2okNgMSt9=5FwEA=3A9_a=3DQEXdDO2ut3YA=3A10_a=3DOJSv?= 8, 40 -- =?us-ascii?q?EavBdRUA=3A10?= 8, 40 -- =?us-ascii?q?_a=3D72aGdUroxwQA=3A10?= 8, 40 -- X-IronPort-Anti-Spam-Filtered: true 8, 40 -- X-IronPort-AV: E=Sophos;i="5.46,536,1511845200"; 8, 40 -- d="scan'208";a="91973747" 8, 40 -- Received: from exchange.purdue.edu ([128.210.1.29]) 8, 40 -- by xppmailspam05.itap.purdue.edu with ESMTP/TLS/AES256-SHA; 19 Feb 2018 16:11:27 -0500 8, 40 -- Received: from wppexc03.purdue.lcl (172.30.136.176) by wppexc03.purdue.lcl 8, 40 -- (172.30.136.176) with Microsoft SMTP Server (TLS) id 15.0.1178.4; Mon, 19 Feb 8, 40 -- 2018 16:11:27 -0500 8, 40 -- Received: from wppexc03.purdue.lcl ([fe80::3c86:dcb8:fd99:f174]) by 8, 40 -- wppexc03.purdue.lcl ([fe80::3c86:dcb8:fd99:f174%15]) with mapi id 8, 40 -- 15.00.1178.000; Mon, 19 Feb 2018 16:11:27 -0500 8, 40 -- From: "Gilpin, Christopher J" {gilpin-at-purdue.edu} 8, 40 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 8, 40 -- Subject: evaporating Tin 8, 40 -- Thread-Topic: evaporating Tin 8, 40 -- Thread-Index: AdOpxe+Q9uh2u7unQXK2MI4XTzAWFA== 8, 40 -- Date: Mon, 19 Feb 2018 21:11:26 +0000 8, 40 -- Message-ID: {3a34e7f3e5834c789a3300e8572f0391-at-wppexc03.purdue.lcl} 8, 40 -- Accept-Language: en-US 8, 40 -- Content-Language: en-US 8, 40 -- X-MS-Has-Attach: 8, 40 -- X-MS-TNEF-Correlator: 8, 40 -- x-ms-exchange-transport-fromentityheader: Hosted 8, 40 -- x-originating-ip: [10.163.23.200] 8, 40 -- Content-Type: text/plain; charset="utf-8" 8, 40 -- MIME-Version: 1.0 8, 40 -- Content-Transfer-Encoding: 8bit 8, 40 -- X-MIME-Autoconverted: from base64 to 8bit by microscopy.com id w1JMFMn2013820 ==============================End of - Headers==============================
As the evaporated films get thicker, internal stress becomes significant. I'm not sure how bad tin films are but I've had issues with the films peeling when they got much over 0.1um thickness.
Good luck, Henk
--------------------
Hendrik O. Colijn Center for Electron Microscopy and AnalysiS The Ohio State University 1305 Kinnear Rd, Suite 100, Columbus, OH 43212
colijn.1-at-osu.edu 614/643-3458 cemas.osu.edu
"Time is that quality of nature which keeps things from happening all at once." (Ray Cummings - 1922) Lately it doesn't seem to be working.
------ Original Message ------ X-from: gilpin-at-purdue.edu To: colijn.1-at-osu.edu Sent: 2/19/2018 5:17:35 PM
Considering that is more than 100 times the thickness you normally put down, that is an issue.
I tried using evaporation to put down a thick layer of antimony on glass before. First, when I calculated the mass of Sb to put down a micron at a certain distance, I found I had to fill my tungsten basket to the full and maybe even run a couple of times. Then I think I found the thermal stresses Henk described. The layer curled up off the glass.
Why do they want such a thickness?
Warren
-----Original Message----- X-from: gilpin-at-purdue.edu [mailto:gilpin-at-purdue.edu] Sent: Monday, February 19, 2018 4:17 PM To: Straszheim, Warren E [BIOTC]
Hi Everyone, I have been asked if our vacuum evaporator will put down tin to 1 micron thickness.
We have a Cressington unit so its not the principle it’s the practice. Does anyone have experience with tin or a thickness of 1 micron or both!
Chris
Christopher J. Gilpin Ph.D. Director, Life Science Microscopy Facility Campus-wide Coordinator for Electron Microscopy Purdue University Whistler Hall of Agriculture Research, Room S052 170 S. University St West Lafayette, IN 47907 765-494-7750 gilpin-at-purdue.edu lsmf-at-purdue.edu reaches everyone in the facility. http://ag.purdue.edu/arp/Microscopy/Pages/default.aspx
==============================Original Headers============================== 8, 40 -- From gilpin-at-purdue.edu Mon Feb 19 16:15:22 2018 8, 40 -- Received: from xppmailspam05.itap.purdue.edu (xppmailspam05.itap.purdue.edu [128.210.5.16]) 8, 40 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w1JMFMn2013820 8, 40 -- for {microscopy-at-microscopy.com} ; Mon, 19 Feb 2018 16:15:22 -0600 8, 40 -- X-Cloudmark-SP-Filtered: true 8, 40 -- X-Cloudmark-SP-Result: =?us-ascii?q?v=3D2=2E1_cv=3DU7Iydbfu_c=3D1_sm=3D2_tr?= 8, 40 -- =?us-ascii?q?=3D0_a=3DdSX5codZc0cA=3A10_a=3DIkcTkHD0fZMA=3A10?= 8, 40 -- =?us-ascii?q?_a=3Dx7bEGLp0ZPQA=3A10_a=3DxqWC=5FBr6kY4A=3A10_a=3DOp4juWPpsa0?= 8, 40 -- =?us-ascii?q?A=3A10_a=3D35Thz0BbAAAA=3A8?= 8, 40 -- =?us-ascii?q?_a=3D99ovruB2okNgMSt9=5FwEA=3A9_a=3DQEXdDO2ut3YA=3A10_a=3DOJSv?= 8, 40 -- =?us-ascii?q?EavBdRUA=3A10?= 8, 40 -- =?us-ascii?q?_a=3D72aGdUroxwQA=3A10?= 8, 40 -- X-IronPort-Anti-Spam-Filtered: true 8, 40 -- X-IronPort-AV: E=Sophos;i="5.46,536,1511845200"; 8, 40 -- d="scan'208";a="91973747" 8, 40 -- Received: from exchange.purdue.edu ([128.210.1.29]) 8, 40 -- by xppmailspam05.itap.purdue.edu with ESMTP/TLS/AES256-SHA; 19 Feb 2018 16:11:27 -0500 8, 40 -- Received: from wppexc03.purdue.lcl (172.30.136.176) by wppexc03.purdue.lcl 8, 40 -- (172.30.136.176) with Microsoft SMTP Server (TLS) id 15.0.1178.4; Mon, 19 Feb 8, 40 -- 2018 16:11:27 -0500 8, 40 -- Received: from wppexc03.purdue.lcl ([fe80::3c86:dcb8:fd99:f174]) by 8, 40 -- wppexc03.purdue.lcl ([fe80::3c86:dcb8:fd99:f174%15]) with mapi id 8, 40 -- 15.00.1178.000; Mon, 19 Feb 2018 16:11:27 -0500 8, 40 -- From: "Gilpin, Christopher J" {gilpin-at-purdue.edu} 8, 40 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 8, 40 -- Subject: evaporating Tin 8, 40 -- Thread-Topic: evaporating Tin 8, 40 -- Thread-Index: AdOpxe+Q9uh2u7unQXK2MI4XTzAWFA== 8, 40 -- Date: Mon, 19 Feb 2018 21:11:26 +0000 8, 40 -- Message-ID: {3a34e7f3e5834c789a3300e8572f0391-at-wppexc03.purdue.lcl} 8, 40 -- Accept-Language: en-US 8, 40 -- Content-Language: en-US 8, 40 -- X-MS-Has-Attach: 8, 40 -- X-MS-TNEF-Correlator: 8, 40 -- x-ms-exchange-transport-fromentityheader: Hosted 8, 40 -- x-originating-ip: [10.163.23.200] 8, 40 -- Content-Type: text/plain; charset="utf-8" 8, 40 -- MIME-Version: 1.0 8, 40 -- Content-Transfer-Encoding: 8bit 8, 40 -- X-MIME-Autoconverted: from base64 to 8bit by microscopy.com id w1JMFMn2013820 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both chrisbrantner-at-gwu.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: chrisbrantner-at-gwu.edu Name: Chris Brantner
Organization: The George Washington University
Title-Subject: [Filtered] GWNIC EM position posted
Message: Greetings fellow microscopists,
I want to draw your attention to a position that I have open in my lab. If you are interested, please follow the links.
https://www.gwu.jobs/postings/49625
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X-from: Matt Russell {Matt.Russell-at-crick.ac.uk}
Hi Jan,
We have a CPD300 here that users have access to. One of our users is only trained on our bench top SEM and she uses it regularly, independently.
Top tips for things that might catch out students;
1. As per the manual, the fillers should be on top of the holders. If you have them the other way round the chamber doesn’t fill up correctly 2. Don’t overfill the chamber. Should be OK as long as the level is below the outlet hole at the top, otherwise you might have some liquid left at the end.
It’s easy to use with a simple user interface; it’s been easy to train people on it. We use it quite a lot and it gives nice results.
Cheers,
Matt
Matt Russell, PhD Senior Laboratory Research Scientist Electron Microscopy STP The Francis Crick Institute 1 Midland Road London NW1 1AT
} On 18 Feb 2018, at 17:36, microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: Factor, Jan {Jan.Factor-at-purchase.edu {mailto:Jan.Factor-at-purchase.edu} } } } } } Dear List: } } I am considering replacing an older critical point dryer for a multi-user microscopy lab that serves } faculty and undergraduate student users for research projects, and trains undergraduates via } courses. After training, users carry out their own specimen preps. } } Two models I抳e come across are the Leica EMCPD300 and the Tousimis Autosamdri-931. } } I would appreciate any comments from folks who have experience with either of these, as well as } suggestions about other models I may have overlooked but should consider. } } Feel free to respond directly to me if you would like at jan.factor-at-purchase.edu } {mailto:jan.factor-at-purchase.edu} } {mailto:jan.factor-at-purchase.edu} . } } --Many thanks, *Jan * } } Jan Robert Factor, Ph.D. } } Professor of Biology } } Purchase College, State University of New York } } Purchase, NY 10577 } } Office: 2016 Natural Science Bldg., 914-251-6659 } } Office Hours (Spring 2018): } } 牋牋燭ues., 11:00-12:00, and Thurs., 1:30-2:30 } } jan.factor-at-purchase.edu {mailto:jan.factor-at-purchase.edu} {mailto:jan.factor-at-purchase.edu} } } /_*Purchase College ranked in the Top 10 public liberal arts colleges nationally* } {http://colleges.usnews.rankingsandreviews.com/best-colleges/rankings/national-liberal-arts-colleges/top-public} _/ } *Please consider the environment before printing this e-mail* } } } ==============================Original Headers============================== } 19, 53 -- From microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} Sun Feb 18 } 10:53:43 2018 } 19, 53 -- Received: from mail-it0-f43.google.com {http://mail-it0-f43.google.com} } (mail-it0-f43.google.com {http://mail-it0-f43.google.com} [209.85.214.43]) } 19, 53 -- by microscopy.com {http://microscopy.com} (8.12.11.20060308/8.12.8) with ESMTP id } w1IGrhv0021068 } 19, 53 -- for {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } ; 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Cutting the slab is dangerous can may compromise the building. It is better to install active vibration isolation. I have a number of SEMs in Thousand Oaks, CA. One LaB6 system is on the 2nd floor. We use Herzan to reach vendor spec resolution with gold on carbon. Regards Gianni ________________________________________________________________________________________________________________________________ Gianpiero Torraca | Sr. Scientist | ATO Forensics | One Amgen Center Drive | Thousand Oaks, CA 91320 | Mailstop B30E-2B | 805.447.7445 “Mistakes are a fact of life. It is the response to the error that counts.” -Nikki Giovanni
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Friday, February 16, 2018 12:46 PM To: Torraca, Gianni {gtorraca-at-amgen.com}
X-from: Darrell Miles {milesd-at-us.ibm.com}
One thing I don't think has been mentioned, is that the soil under many slab floors has settled, and the slab is "floating" above without being supported. I have heard of holes being drilled and a foam material being injected under the slab to support and dampen vibrations. This is done sometimes, in addition to other vibration isolation measures.
Regards, Darrell
microscopy.listserver-at-gmail.com wrote on 02/18/2018 11:56:14 AM:
} From: microscopy.listserver-at-gmail.com } To: milesd-at-us.ibm.com } Date: 02/18/2018 10:52 AM } Subject: [Microscopy] Fwd: viaWWW: Vibrations: cutting the slab? } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- https://urldefense.proofpoint.com/v2/ } url? } u=http-3A__www.microscopy.com_MicroscopyListserver&d=DwIBAg&c=jf_iaSHvJObTbx- } siA1ZOg&r=4k8urptLaVicBf1XZlZhMTu6vEfbbdyViST2saXOc5I&m=g9zPYPHRNYKozDFlGz7fVlDdc68JHnB1iQn_xboDrY0&s=ugoYKYaSGvJRp_JnumnnfXAPUEYtdF3kIkvZE95DNGc&e= } On-Line Help https://urldefense.proofpoint.com/v2/url? } u=http-3A__www.microscopy.com_MicroscopyListserver_FAQ.html&d=DwIBAg&c=jf_iaSHvJObTbx- } siA1ZOg&r=4k8urptLaVicBf1XZlZhMTu6vEfbbdyViST2saXOc5I&m=g9zPYPHRNYKozDFlGz7fVlDdc68JHnB1iQn_xboDrY0&s=FuLt6nOPYiSRyf8ejzJ0bIoZcEAz8a- } G0wqvuK3-bd0&e= } ---------------------------------------------------------------------------- } } X-from: Henk Colijn {colijn.1-at-osu.edu} } Reply-To: Henk Colijn {colijn.1-at-osu.edu} } To: microscopy.listserver-at-gmail.com } } Hi Bryan, } } Those are very low frequencies. They should be in a range that an } active compensation system should } be able to handle it. } } Another thing to consider is the resonant frequency of the slab } (even though it is damped by the } ground). The more mass in the slab, the lower its resonant } frequency. The install guides for our } microscopes show that the microscope is much more sensitive at very } low frequencies. The allowable } vibration at 10Hz is approx 10x lower than at 20Hz. Since too much } mass can push the slab resonance } down into the range where the microscope is more sensitive you may } want to estimate the resonant } frequency of your current slab and then consider whether to slice it } or not. It would also be } useful to measure the ground vibration away from the instrument to } get an idea of the driving } frequencies. } } Good luck! } Henk } } } } -------------------- } } Hendrik O. Colijn } Center for Electron Microscopy and AnalysiS } The Ohio State University } 1305 Kinnear Rd, Suite 100, Columbus, OH 43212 } } colijn.1-at-osu.edu 614/643-3458 } cemas.osu.edu } } "Time is that quality of nature which keeps things from happening } all at once." (Ray Cummings - 1922) } Lately it doesn't seem to be working. } } ------ Original Message ------ } X-from: microscopy.listserver-at-gmail.com } To: colijn.1-at-osu.edu } Sent: 2/16/2018 4:45:19 AM } Subject: [Microscopy] viaWWW: Vibrations: cutting the slab? } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- } } https://urldefense.proofpoint.com/v2/url? } u=http-3A__www.microscopy.com_MicroscopyListserver&d=DwIBAg&c=jf_iaSHvJObTbx- } siA1ZOg&r=4k8urptLaVicBf1XZlZhMTu6vEfbbdyViST2saXOc5I&m=g9zPYPHRNYKozDFlGz7fVlDdc68JHnB1iQn_xboDrY0&s=ugoYKYaSGvJRp_JnumnnfXAPUEYtdF3kIkvZE95DNGc&e= } } On-Line Help https://urldefense.proofpoint.com/v2/url? } u=http-3A__www.microscopy.com_MicroscopyListserver_FAQ.html&d=DwIBAg&c=jf_iaSHvJObTbx- } siA1ZOg&r=4k8urptLaVicBf1XZlZhMTu6vEfbbdyViST2saXOc5I&m=g9zPYPHRNYKozDFlGz7fVlDdc68JHnB1iQn_xboDrY0&s=FuLt6nOPYiSRyf8ejzJ0bIoZcEAz8a- } G0wqvuK3-bd0&e= } } ---------------------------------------------------------------------------- } } } } X-from: btracy-at-eag.com } } } } This Question/Comment was submitted to the Microscopy Listserver } } using the WWW based Form at https://urldefense.proofpoint.com/v2/ } url? } u=http-3A__www.microscopy.com_MLFormMail.html&d=DwIBAg&c=jf_iaSHvJObTbx- } siA1ZOg&r=4k8urptLaVicBf1XZlZhMTu6vEfbbdyViST2saXOc5I&m=g9zPYPHRNYKozDFlGz7fVlDdc68JHnB1iQn_xboDrY0&s=0KEhg0YZd6xdGCuQ7_HIO_5vtgDgqDECxmHRxO0a1sM&e= } } --------------------------------------------------------------------------- } } Remember this posting is most likely not from a Subscriber, so when } } replying please copy both btracy-at-eag.com as well as the Microscopy } } Listserver } } --------------------------------------------------------------------------- } } } } Email: btracy-at-eag.com Name: Bryan Tracy } } } } Organization: EAG Laboratories } } } } Title-Subject: [Filtered] cutting the slab? } } } } Message: Hello Everyone, } } } } I know this is a subject on which reasonable people can disagree, but I } } wanted to ask what has been your experience with cutting the slab to } } reduce low frequency vibrations? } } } } The floor appears to be moving in the horizontal plane at 1.6Hz (4.4 } } reduction factor needed) and in the vertical direction at 5HZ. 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X-from: LNMA CMN Siglo XXI {lnma.cmn.sigloxxi-at-gmail.com}
Hi, Lisa. I am not an expert, but I would choose an analysis method knowing whether I will need to keep the seed, or it can be used entirely. Maybe I would try splitting it in parts (yep, it's millet, but still..) and then try different several fixation methods. I would also probably opt for hydration of the seed before fixation, but on an EtOH — to prevent germination[?] or fungal growth vapor bath. Interesting task!
Cheers, Vad
On Mon, Feb 19, 2018 at 9:03 AM, {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote:
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X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu {mailto:oshel1pe-at-cmich.edu} } To: microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} }
Lisa,
Good luck. I like Rosemary’s suggestion — mine about liquid nitrogen needs decent sized seeds. I’ve used it for corn and barley, but if you do have millet … oof.
Phil ------------- Philip Oshel Imaging Facility Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 989 774-3576 office 989 774-7567 lab
-----Original Message----- X-from: "microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} " {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } Reply-To: "microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} " {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } Date: Sunday, 18February, 2018 at 12:29 To: Philip Oshel {oshel1pe-at-cmich.edu {mailto:oshel1pe-at-cmich.edu} } Subject: [Microscopy] Fwd: Re: viaWWW: ancient grain imaging help needed
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X-from: Lisa Anne O'Donovan {lisa.odonovan-at-adelaide.edu.au {mailto:lisa.odonovan-at-adelaide.edu.au} }
Thanks Phil. I’ve imaged grain before and I’ve found the best result after soaking it for 48 hrs in water and then cutting it into pieces before fixing and dehydration. Unfortunately I have no idea if soaking this grain might have a more detrimental effect. Thanks for your advice
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Title-Subject: [Filtered] Two seats left! - BIO TEM workshop
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Hello, I am wondering if anybody can reccomend a standard/sample for determining microscope resolution in a SEM using the Fourier Ring Correlation (Autocorrelation) method. It is my understanding that suitable samples for TEM resolution estimates using FRC will not work in an SEM due to the differences in contrast mechanisms. Ideally, these would be samples which exhibit spatial frequencies from DC up to and beyond the resolution limit of the microscope.
Thank You, Nathaniel Rieders
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both rabara-at-tescan-usa.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: rabara-at-tescan-usa.com Name: Michal Rabara
Organization: TESCAN USA, Inc.
Title-Subject: [Filtered] Microscopy Position Posted
Message: Organization: Tescan USA, Inc.
Email response: rabara-at-tescan-usa.com Michal Rabara, CEO & President Tescan USA, Inc.
I would like to draw your attention to the Applications Manager position that we have open in North America for Tescan USA, Inc. If you are interested, please follow the link below:
https://www.linkedin.com/jobs/view/602245101/
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Email: jcaughey-at-su-group.com Name: Jim Caughey
Title-Subject: [Filtered] Zeiss Libra
Message: Does anyone know of a qualified ISO that can support this TEM - Zeiss Libra 120 Plus TEM?
Thanks for any help!
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Email: sorin.lazar-at-fei.com Name: Sorin Lazar
Organization: Thermo Fisher Scientific
Title-Subject: [Filtered] Applications Scientist TEM Semiconductors at Thermo Fisher Scientific, Eindhoven
Message: Dear TEM community,
I would like to draw your attention to an Applications Scientist TEM Semiconductors open position at Thermo Fisher Scientific in the Eindhoven Nanoport.
For those interested please follow the link below:
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Email: leonarddn-at-ornl.gov Name: Donovan Leonard
I am interested in learning what the potential cost of ownership of a Xe PFIB can be. In addition to the service contract, what costs associated with apertures and sources are there? I am assuming apertures have to be changed more frequently since beam currents can be on the order of microamps (depending on hours in use). What about the Xe gas? I think this is just a small lecture bottle of gas, but how often do you have to replenish that source?
Please respond off list to leonarddn-at-ornl.gov and thank you in advance. If you want to include any anecdotes about Xe PFIB ownership I am happy to learn about that too (e.g. nightmares or winning scenarios).
Donovan
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Email: sorin.lazar-at-fei.com Name: Sorin Lazar
Organization: Thermo Fisher Scientific
Title-Subject: [Filtered] job opening
Message:
"Dear TEM community,
I would like to draw your attention to an Applications Scientist TEM Materials Science open position at Thermo Fisher Scientific in the Eindhoven Nanoport.
For those interested please follow the link below: http://jobs.thermofisher.com/ShowJob/Id/156547/Applications-Scientist-TEM-Materials-Science/
Thanks and regards, Sorin Lazar "
With many thanks in advance, Sorin
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I just change the filament on a FEI Morgagni TEM. As I put the filament on the microscope and close the collum the PVP does not start.
I reset the system, open the column again, I have no idea of what to do next. Have anyone already had this problem? Any tips?
Thank you for your attention.
Best,
Pedro Leão
-- ---
Instituto de Microbiologia - UFRJ Laboratório de Biologia Celular e Magnetotaxia CCS - Centro de Ciências da Saúde - Bloco I Avenida Carlos Chagas Filho, 373 Cidade Universitária CEP. 21941-902 Rio de Janeiro - RJ - Brasil CV Lattes: http://lattes.cnpq.br/9781167318809464 LinkedIn:https://br.linkedin.com/in/pedro-leão-55992853 {https://br.linkedin.com/in/pedro-le%C3%A3o-55992853} Tel. +55 21 3938 6738 Cel. +55 21 98131 3360
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Email: millerme-at-ornl.gov Name: Madalynn Miller
Organization: Oak Ridge National Laboratory
Title-Subject: [Filtered] ORNL Seeks Postdoc, Helium Ion Microscopy
Message: Postdoctoral Research Associate in Helium Ion Microscopy
Oak Ridge National Laboratory (ORNL) is looking for a Postdoctoral Research Associate to conduct Helium Ion Microscopy (HIM) research and Secondary Ion Mass Spectrometry (SIMS) of a broad range of inorganic and soft materials. Specifically, your research in this role will focus on imaging of soft materials. For this role, experience in mass spectrometry (MS) and helium ion microscopy (HIM) techniques, and/or other closely related areas is ideal. You will work with scientists and users at the CNMS in developing new HIM capabilities in chemical imaging using SIMS, multimodal data analysis, and integration with high performance computing environments. You will benefit from experience in the analysis and characterization of soft materials and inorganic materials.
For more details, including the qualifications for this position, visit: http://bit.ly/PostdocHIM
ORNL is an equal opportunity employer. All qualified applicants, including individuals with disabilities and protected veterans, are encouraged to apply.
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X-from: Gary Laevsky {glaevsky.lists-at-gmail.com}
Hi All!
We are very proud and excited to announce the formation of a new society, The North Atlantic Microscopy Society (NAMS). Geographically centered at Princeton, NJ, we envision our coverage to span southern New York, New Jersey and into Pennsylvania.
Edwin Hubble famously said, “Equipped with his five senses, man explores the universe around him and calls the adventure Science.” We believe that some of this exploratory instinct has been muted lately by our disciplinary silos. Individually, we have become exceptional in our specialties and do not take moments to appreciate the many discoveries happening across the entire spectrum of science.
Our mission is to bridge these silos through the lens of microscopy. We seek to achieve this mission by promoting microscopy education, stimulating networking among microscopists, and disseminating microscopy knowledge and skills to the public in the region.
Our first Symposium will be held at Princeton University on November 1, 2018.
Our first speakers will be;
Hari Shroff (Light) - Chief of NIBIB's Section on High Resolution Optical Imaging laboratory. Nieng Yan (Cryo-EM) - Shilrley M. Tilghman Professor of Molecular Biology at Princeton University.
This is exciting!!!
Gary Laevsky (Light Imaging) Paul Shao (EM Imaging) Tharan Srikumar (Mass Spec Imaging)
-- Best,
Gary Laevsky, Ph.D. Director, Confocal Imaging Facility Nikon Center of Excellence Dept. of Molecular Biology Washington Rd. Princeton University Princeton, New Jersey, 08544-1014 (O) 609 258 5432 (C) 508 507 1310
Greetings The deadline for paper submission has passed and meeting registration is now open. As you are making plans to attend the Baltimore, MD meetings (Aug. 5-9) please don't forget about MSA's student bursary program. Its purpose is to encourage students to attend the meetings by helping to defray some of the costs while giving them an opportunity to meet and interact with the established microscopy community.
The student bursaries will be paid $10 an hour to work for ~20 hours during the meeting or pre-meeting events (paid by check at the end of the meetings). The jobs involve such things as providing support in the different symposia, staffing the volunteer office, newsletter distribution, and helping with vendor tutorial sign-up or in the outreach booth.
Once the program has been finalized, each registered bursary will be contacted and given the chance to choose the times and activities they would like to help with. There is an additional bonus of $10 cash for each morning and/or afternoon session worked to assist with meals, along with a meeting t-shirt.
If anyone would like to participate in the bursary program, please check the box "I wish to apply for a student bursary" on the registration form. Applicants for the bursaries must be a member of MSA or MAS and enrolled as a student at a recognized educational institution. Participants are responsible for their own registration fee and travel expenses.
For those of you who may not be students, but would still like to assist with the meetings, volunteers are also needed to fill the above-mentioned jobs. Although not paid the $10/hour as the students, volunteers do get a meeting t-shirt and the $10 cash for each morning/afternoon worked to help with meals.
If anyone has any questions about the bursary/volunteer program, or would like to participate, contact Amanda Lawrence: mailto:aml1819454-at-gmail.com (please do not reply to the email associated with this post). Bursary space is limited, so sign-up early.
See everyone in Baltimore,
Amanda Lawrence Student Bursary/Volunteer Coordinator Microscopy Society of America mailto:aml181954-at-gmail.com
==============================Original Headers============================== 10, 31 -- From ALawrence-at-i2at.msstate.edu Fri Mar 2 07:52:41 2018 10, 31 -- Received: from catalpa.its.msstate.edu (catalpa.its.msstate.edu [130.18.2.119]) 10, 31 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w22DqfcQ003854 10, 31 -- for {microscopy-at-microscopy.com} ; Fri, 2 Mar 2018 07:52:41 -0600 10, 31 -- Received: from mail03.ad.msstate.edu (mail03.ad.msstate.edu [130.18.230.62]) 10, 31 -- by catalpa.its.msstate.edu (8.14.7/8.14.7) with ESMTP id w22CnLY9055835 10, 31 -- (version=TLSv1/SSLv3 cipher=ECDHE-RSA-AES256-SHA384 bits=256 verify=FAIL) 10, 31 -- for {microscopy-at-microscopy.com} ; Fri, 2 Mar 2018 06:49:21 -0600 10, 31 -- Received: from MAIL02.ad.msstate.edu (2620:0:1a30:1230::61) by 10, 31 -- mail03.ad.msstate.edu (2620:0:1a30:1230::62) with Microsoft SMTP Server (TLS) 10, 31 -- id 15.0.1347.2; Fri, 2 Mar 2018 06:49:21 -0600 10, 31 -- Received: from MAIL02.ad.msstate.edu ([fe80::7846:3039:9492:24b0]) by 10, 31 -- mail02.ad.msstate.edu ([fe80::7846:3039:9492:24b0%13]) with mapi id 10, 31 -- 15.00.1347.000; Fri, 2 Mar 2018 06:49:21 -0600 10, 31 -- From: "Lawrence, Amanda" {ALawrence-at-i2at.msstate.edu} 10, 31 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 10, 31 -- Subject: Student help at the M&M 2018 meetings 10, 31 -- Thread-Topic: Student help at the M&M 2018 meetings 10, 31 -- Thread-Index: AdOyJN8aMcsLcBwdRPuv+ecrYrWhfA== 10, 31 -- Date: Fri, 2 Mar 2018 12:49:20 +0000 10, 31 -- Message-ID: {9bc64f025adc4dc2b9afd7f6f4ac6db9-at-mail02.ad.msstate.edu} 10, 31 -- Accept-Language: en-US 10, 31 -- Content-Language: en-US 10, 31 -- X-MS-Has-Attach: 10, 31 -- X-MS-TNEF-Correlator: 10, 31 -- x-ms-exchange-transport-fromentityheader: Hosted 10, 31 -- x-originating-ip: [130.18.230.93] 10, 31 -- Content-Type: text/plain; charset="us-ascii" 10, 31 -- MIME-Version: 1.0 10, 31 -- Content-Transfer-Encoding: 8bit 10, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id w22DqfcQ003854 ==============================End of - Headers==============================
I am hoping someone could recommend somewhat-reliable software or (open source plug-in preferred) for extracting (approximated) 2D PSF from a pair of images of the same object, taken with closely matched contrast and same digital pixel count, but different beam current/diameter/profile. One of the images would be significantly sharper and serve as a reference, while the other "blurry" one is assumed to be a convolution of the reference image and PSF of the beam/instrument. The interest would be in extracting and examining PSF from the pair of images, convoluting extracted PSF with sharp image to estimate blurred one, and de-convoluting PSF from the blurred image to reconstruct sharp version. I am finding multiple plugins, but don't seem to come across something that would be convenient for all three operations.
Thank you very much beforehand,
Valery Ray ============================== PBS&T, MEO Engineering Company 290 Broadway, Suite 298 Methuen, MA 01844, USA Phone: +1-978-305-0479 - leave a message Mobie: +1-978-305-0479 - leave a message E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
I wanted to let you know that we’ve got two openings here at PNNL for an SEM technician and technologist to support imaging and sample preparation of nuclear materials. Candidates with a background in materials science and electron microscopy would be ideal.
Please feel free to forward the following links to anyone you think might be interested and don’t hesitate to contact me with any questions. Thank you.
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Message: Technical Associate Staff Member - Scanning Probe Microscopy
Oak Ridge National Laboratory (ORNL) seeking a Technical Associate Staff Member to support scanning probe microscopy efforts at the Center for Nanophase Materials Sciences (CNMS) at Oak Ridge National Laboratory (ORNL). In this role, you will be part of the Scanning Probe Microscopy Group, which is a leader in the development and application of scanning probe microscopies and spectroscopies to image and manipulate materials functionality in complex materials, ionic and electronic conductors, molecular assemblies, and nanoscale structures. A suite of 18 commercial and internally developed atomic force microscopes in ambient and controlled environments and scanning tunneling microscopes in ultrahigh vacuum systems are used for internal and user science, with emerging opportunities in enhanced data analytics and multimodal/chemical imaging.
For more details, including the qualifications for this position, visit: http://bit.ly/TP-SPM ORNL is an equal opportunity employer. All qualified applicants, including individuals with disabilities and protected veterans, are encouraged to apply.
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Email: kristian.molhave-at-nanotech.dtu.dk Name: Kristian Molhave
Organization: DTU Nanotech
Title-Subject: [Filtered] PhD scholarship in In-situ TEM and Applications of Nanowire Vapor Phase Epitaxy Microsystems (VPE)
Message: We have a new PhD position open and I hope you will announce it on your website to help recruiting!
PhD scholarship in In situ TEM and Applications of Nanowire Vapor Phase Epitaxy Microsystems (VPE)
please apply online via http://www.nanotech.dtu.dk/about-ntch/ledige-stillinger/job?id=fd61bf71-1ffa-4e21-9a8c-d01c4c5f19a7
In this PhD project you will develop, fabricate and use microfabricated heater systems to study the processes of III-V nanowire growth with MOVPE in-situ TEM, thereby creating nanowire devices while imaging the process live!
Contact information: - Kristian Mlhave, DTU Nanotech, e-mail: kristian.molhave-at-nanotech.dtu.dk - Kimberly Dick Thelander, Solid State Physics, Lund University, e-mail: kimberly.dick_thelander-at-ftf.lth.se - Nika Akopian, DTU Photonics, e-mail: nikaak-at-fotonik.dtu.dk
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Email: dlowry-at-asu.edu Name: David Lowry
Organization: Arizona State University
Title-Subject: [Filtered] thermal treatment of carbon-formvar film
Message: a researcher here wants to do some thermal treatments (up to 150C) of samples after they have been adhered to carbon-formvar coated silicon wafers (approx 13mm x 36mm) containing small holes, and inquired with me about the max temp these films can endure without failing. She has tested a blank film at her max temp and the film appeared to remain intact during heating, but fell apart when removed from oven and during cool down. We have searched for published info on thermal resistance of carbon-formvar but could not locate anything specific, so we are turning to the list for advice. The films are home-made and approx 25-30 nm thick for the formvar and approx 10nm carbon. thank you- Login Host: 149.169.81.41 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
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Message: Research Associate Electron Microscopy Technologist
Cold Spring Harbor Laboratory seeks a highly motivated dedicated individual to work in a state-of-the-art Microscopy Shared Resource.
The individual should have extensive practical expertise in biological sample preparation for transmission and scanning electron microscopy of animal tissues and mammalian cell lines. Hands-on knowledge of confocal and widefield fluorescence microscopy would also be a plus.
The candidate will help users design innovative experiments and they will carry out sample preparation and imaging as well as assist in data interpretation.
Excellent verbal and written communication skills, ability to work with multiple users in a supporting role, and ability to work independently and proactively with limited supervision are essential. A Bachelors degree in biology or related discipline is required. One to three years of experience working in a Microscopy Shared Resource is preferred.
How to Apply
Interested individuals should apply for this position via the CSHL careers website at http://cshl.peopleadmin.com/postings/11688
Position Number 01779-R
Applicants should include a resume along with a description of their practical expertise and the names as well as email addresses of 3 references.
Cold Spring Harbor Laboratory is a world-renowned research and educational institution recognized internationally for its excellence in ground-breaking research programs in cancer, neuroscience, plant biology, genomics, and bioinformatics and broad educational mission.
For more information about CSHL, please visit us at www.cshl.edu
CSHL is an EO/AA Employer. All qualified applicants will receive consideration for employment and will not be discriminated against on the basis of race, color, religion, sex, sexual orientation, gender identity, national origin, age, disability or protected veteran status.
VEVRAA Federal Contractor
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Message: Dear listers. My first question is- Is osmium better purchased made up in vials or made from crystals in the lab. I myself prefer the vials, I feel they are safer and the solution is protected from degradation.
Second question. Does UA power degrade over time? We have a bottle that is 2+ years old, purchased from Polysciences.
Thank you in advance.
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Email: nyao-at-princeton.edu Name: Nan Yao
Organization: Princeton University Title-Subject: [Filtered] Princeton-Nature Conference: Frontiers in Electron Microscopy for the Physical and Life Sciences
Message: I would like to ask your help for including the news of a new Nature Conference in electron microscopy, in your future news update. Website link: http://www.nature.com/natureconferences/fempl2018/index.html
This new conference is entitled Princeton Nature Conference: Frontiers in Electron Microscopy for the Physical and Life Sciences, to be held at Princeton, New Jersey, July 11-13, 2018. Many internationally renowned scientists including 2017 Nobel Prize winner Dr. Joachim Frank will speak in this conference.
Thank you for your attention. Your help in posting this in the MSA community is greatly appreciated.
Best regards,
Nan Yao
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=} Osmium: I have the impression that 4% OsO4 (stored in sealed glass ampoules under nitrogen at 4 Celsius) staines less strong after storage for over 20 years (!); the crystalline form workswell after storage for more than 20 years (small tip for dissolving: I snap off the tip of the glass vial, add the water, seal tip with a little amount of parafilm/nescofilm and sonicate in in ultrasonic waterbath for ca 5 minutes(in fumehood))
=} Uranylacetate: I use UA bought in the early nineties, works perfectly...
greetings, Peter Heimann ( Cell Biology; Bielefeld University; Germany
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==============================Original Headers============================== 7, 26 -- From peter.heimann-at-uni-bielefeld.de Thu Mar 8 08:16:15 2018 7, 26 -- Received: from unibi-smtp-a.hrz.uni-bielefeld.de (unibi-smtp-a.hrz.uni-bielefeld.de [129.70.208.12]) 7, 26 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w28EGEYj014190 7, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 8 Mar 2018 08:16:14 -0600 7, 26 -- MIME-version: 1.0 7, 26 -- Content-transfer-encoding: 8BIT 7, 26 -- Content-type: text/plain; charset=iso-8859-15; format=flowed 7, 26 -- Received: from [192.168.178.21] ([217.247.95.107]) 7, 26 -- by unibi-smtp-a.hrz.uni-bielefeld.de 7, 26 -- (Oracle Communications Messaging Server 7.0.5.37.0 64bit (built Jan 25 2016)) 7, 26 -- with ESMTPPA id {0P590051KWPWB160-at-unibi-smtp-a.hrz.uni-bielefeld.de} for 7, 26 -- Microscopy-at-microscopy.com; Thu, 08 Mar 2018 14:13:14 +0100 (CET) 7, 26 -- X-Connecting-IP: [217.247.95.107] 7, 26 -- X-PMX-Version: 6.3.3.2656215, Antispam-Engine: 2.7.2.2107409, 7, 26 -- Antispam-Data: 2018.3.8.130615, AntiVirus-Engine: 5.46.1.0, 7, 26 -- AntiVirus-Data: 2018.3.8.5461002, pmx12 7, 26 -- X-EnvFrom: peter.heimann-at-uni-bielefeld.de 7, 26 -- Subject: Re: Osmium and UA question ( Peter Heimann ) 7, 26 -- To: Microscopy-at-microscopy.com 7, 26 -- References: {201803080449.w284nJC9011969-at-microscopy.com} 7, 26 -- From: Peter Heimann {peter.heimann-at-uni-bielefeld.de} 7, 26 -- Message-id: {330a3059-2895-00c0-00bf-57d52b75f13f-at-uni-bielefeld.de} 7, 26 -- Date: Thu, 08 Mar 2018 14:13:01 +0100 7, 26 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:52.0) Gecko/20100101 7, 26 -- Thunderbird/52.6.0 7, 26 -- In-reply-to: {201803080449.w284nJC9011969-at-microscopy.com} ==============================End of - Headers==============================
In our hands, we always work with 1 g Osmium crystal (sealed ampoules). As we have a strong sample preparation load in the lab, the final solution (1-2%) is consumed in the next month aproximately, but I suppose that it is very stable.
In Uranile case, we have been using the powder from a bottle with more than 20 years without notice any degradation related to a new one.
In my opinion, the two substances are very stable over time.
Best regards
Juan Luis
El 08/03/2018 a las 6:08, microscopy.listserver-at-gmail.com escribió: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe --http://www.microscopy.com/MicroscopyListserver } On-Line Helphttp://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from:Buchsmith-at-gmail.com } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form athttp://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } Buchsmith-at-gmail.com as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email:Buchsmith-at-gmail.com Name: JoAnn Buchanan } } Organization: Allen Institute } } Title-Subject: [Filtered] Osmium and UA question } } Message: Dear listers. } My first question is- Is osmium better purchased made up in vials or made from crystals in the lab. } I myself prefer the vials, I feel they are safer and the solution is protected from degradation. } } Second question. Does UA power degrade over time? We have a bottle that is 2+ years old, purchased } from Polysciences. } } Thank you in advance. } } Login Host: 63.237.233.8 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 11, 53 -- Frommicroscopy.listserver-at-gmail.com Wed Mar 7 22:48:42 2018 } 11, 53 -- Received: from mail-io0-f175.google.com (mail-io0-f175.google.com [209.85.223.175]) } 11, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w284mfBi011663 } 11, 53 -- for {microscopy-at-microscopy.com} ; Wed, 7 Mar 2018 22:48:42 -0600 } 11, 53 -- Received: by mail-io0-f175.google.com with SMTP id 30so5513988iog.2 } 11, 53 -- for {microscopy-at-microscopy.com} ; Wed, 07 Mar 2018 19:45:41 -0800 (PST) } 11, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 11, 53 -- d=gmail.com; s=20161025; } 11, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 11, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 11, 53 -- bh=f/Yu+VKdj9y3E27tzkveluSivokBGnkSx8d2uxvn0RQ=; } 11, 53 -- b=iX2qadp8mIy41M/gqeZJ2271E5oUA5yYpPWUoSaWMl1sQcPpMPCTRSXWqdvc0QupDP } 11, 53 -- n/bIyCMIk3tBZJQGcNJTk8u3jd1Zde5bd97JihT3F+5YHqJ/ScegQSgBO5uB4cmrvFH9 } 11, 53 -- K/cE/M9izr7JkgOuLcjbZhy/JAYnMRXZ7UI8x0C0vLWp+JpPJEXiPve5lzD8CJKggjz0 } 11, 53 -- tjU0TQHfZIiFoGd4+eu7lfFUvcdsDw85XyfXkNAlXgDfBB0nAARpLQ4g0gMLY9X651vu } 11, 53 -- LOM2GffkQW/rtU7ZUGSo9V0Y6MkrFQ0p9YpzQMKX71Afgm3RPSQeJVAHCg39vWRaLW+L } 11, 53 -- dJiw== } 11, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 11, 53 -- d=1e100.net; s=20161025; } 11, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 11, 53 -- :user-agent:mime-version:in-reply-to:content-language } 11, 53 -- :content-transfer-encoding; } 11, 53 -- bh=f/Yu+VKdj9y3E27tzkveluSivokBGnkSx8d2uxvn0RQ=; } 11, 53 -- b=EAgVy0BoLXJ+ACYWgFOarLTOzRL7xtXPoPo/I1TSae5ZR4ka6PLF/YUzD6Y50BxRNJ } 11, 53 -- g01jSJo46q27ZpbNE/oY4u6Jdn+564gtUf3VvMPW1NN6W9XHIWFtpcrDe60WK4vxn8S9 } 11, 53 -- KH+2+aswmLZBJPfJl+lVh6nwv2OMVPXf+tqa2I7bPdXjR1w7LeWydqQbV/F/66zS2s+d } 11, 53 -- hJffVVoxpEp9IDvsXLXcBOm4W2QROQpJXsktTvQRZIr05ouXXtE+4HSXnn6je6XpfZDP } 11, 53 -- wniIJEy8fRGnB/LOBXHfkFlMETy6yoNqIDi9iP5OlhKj3WKUA7xVJfU4y6lBC3eKansk } 11, 53 -- YXDQ== } 11, 53 -- X-Gm-Message-State: APf1xPBwRWzuybdDmi49WClSLUFKMxR2S/k8wqXpD32ggf1AB+3h2lS4 } 11, 53 -- ebNfiLU5eUqbS9kvhNBfqRO6wo4h } 11, 53 -- X-Google-Smtp-Source: AG47ELvgfOZaRlUqNP9EllVdzSQbhZmL9D443zO9ij3nLRuiON0IOAY0OnD+6bCLSbcANCC4efArGQ== } 11, 53 -- X-Received: by 10.107.38.80 with SMTP id m77mr29464806iom.69.1520480740535; } 11, 53 -- Wed, 07 Mar 2018 19:45:40 -0800 (PST) } 11, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:14f1:a005:3aea:e3a8]) } 11, 53 -- by smtp.googlemail.com with ESMTPSA id 38sm13350272iom.48.2018.03.07.19.45.39 } 11, 53 -- for {microscopy-at-microscopy.com} } 11, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 11, 53 -- Wed, 07 Mar 2018 19:45:39 -0800 (PST) } 11, 53 -- Subject: viaWWW: Osmium and UA question } 11, 53 -- References: {201803080201.w2821DPe001173-at-microscopy.com} } 11, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 11, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 11, 53 -- X-Forwarded-Message-Id: {201803080201.w2821DPe001173-at-microscopy.com} } 11, 53 -- Message-ID: {dba7c4ff-11f6-8316-ce10-3e46ddb4b0d4-at-gmail.com} } 11, 53 -- Date: Wed, 7 Mar 2018 21:45:38 -0600 } 11, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) } 11, 53 -- Gecko/20100101 Thunderbird/52.6.0 } 11, 53 -- MIME-Version: 1.0 } 11, 53 -- In-Reply-To: {201803080201.w2821DPe001173-at-microscopy.com} } 11, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 11, 53 -- Content-Language: en-US } 11, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
-- /Juan Luis Ribas/ Servicio de Microscopía Centro de Investigación, Tecnología e Innovación Universidad de Sevilla Av. Reina Mercedes 4b 41012 Sevilla
Hi - want to share my experience with UA. About 10 yrs ago, I inherited a bottle of UA dated 1960s. I used it to do girds staining and negative staining. It did stain without any noticeable problems but then I got a new bottle UA of EMS from another PI, with which my staining looked fresher and less background. The bottom line is fresh US does give better staining. Your two-year-old UA should just be fine. Greg ---------------------------- Gang (Greg) Ning, Ph.D. Director, Microscopy Facility The Huck Institutes of the Life Sciences The Pennsylvania State University N-048 MSC University Park, PA 16802 USA Phone: 814-863-0994 Fax: 914-867-2587 Email: gxn7-at-psu.edu http://www.huck.psu.edu/facilities/microscopy-cytometry-up
----- Original Message ----- X-from: "microscopy listserver" {microscopy.listserver-at-gmail.com} To: "Gang Ning" {gxn7-at-psu.edu} Sent: Thursday, March 8, 2018 12:12:12 AM
X-from: Buchsmith-at-gmail.com
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Message: Dear listers. My first question is- Is osmium better purchased made up in vials or made from crystals in the lab. I myself prefer the vials, I feel they are safer and the solution is protected from degradation.
Second question. Does UA power degrade over time? We have a bottle that is 2+ years old, purchased from Polysciences.
Thank you in advance.
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Message: Technical Associate Staff Member - Scanning Probe Microscopy
Oak Ridge National Laboratory (ORNL) seeking a Technical Associate Staff Member to support scanning probe microscopy efforts at the Center for Nanophase Materials Sciences (CNMS) at Oak Ridge National Laboratory (ORNL). In this role, you will be part of the Scanning Probe Microscopy Group, which is a leader in the development and application of scanning probe microscopies and spectroscopies to image and manipulate materials functionality in complex materials, ionic and electronic conductors, molecular assemblies, and nanoscale structures. A suite of 18 commercial and internally developed atomic force microscopes in ambient and controlled environments and scanning tunneling microscopes in ultrahigh vacuum systems are used for internal and user science, with emerging opportunities in enhanced data analytics and multimodal/chemical imaging.
For more details, including the qualifications for this position, visit: http://bit.ly/TP-SPM ORNL is an equal opportunity employer. All qualified applicants, including individuals with disabilities and protected veterans, are encouraged to apply.
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Email: rvancamp-at-kettering.edu Name: Rick Van Camp
Organization: Kettering University
Title-Subject: [Filtered] Training Inquiry Re: Leica Reichert Ultracut S
Message: We need to hasten our use of our ultramictrotome. I have identified a few of these still in use but, need more input to find facilities using this instrument on a daily basis that are also willing to train me in its operation. I have made progress on my own yet, have only been able to find one facility willing to provide training. I have also used the manual and made progress yet, I cannot state the manual for this instrument is well written. Finally, it is best for me to locate facilities within the Great Lakes region. I have searched for online training for this and have been unsuccessful.
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From purchase-at-upscmarine.com.sg Fri Mar 9 13:14:02 2018 Return-Path: {purchase-at-upscmarine.com.sg} Received: from 5t3.com (host42-30-237-212.serverdedicati.aruba.it [212.237.30.42] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w29JE1HJ030478; Fri, 9 Mar 2018 13:14:01 -0600 Message-Id: {201803091914.w29JE1HJ030478-at-microscopy.com} Received: from [103.207.38.155] (unknown [103.207.38.155]) by 5t3.com (Postfix) with ESMTPA id 7AC1E4DB3D; Thu, 8 Mar 2018 14:26:28 +0100 (CET) Content-Type: multipart/mixed; boundary="===============0965157591==" MIME-Version: 1.0
Once again I find myself turning to this most amazing of resources. I am casting a wide net for a micro CT operator and need your collective assistance. Many of you are closely associated with your local CT facilities or at least know the individuals providing the services. I am asking if you would be willing to pass this announcement along as I have not yet found a resource like this one in the scientific CT community.
The Smithsonian Institution National Museum of Natural History (NMNH) in Washington DC has an exciting opportunity to add a cone beam X-ray micro-computed tomography (mCT) operator in support of our new micro-CT Lab. The position is currently a 6 month contract with 2 options to extend providing for the scanning and modelling of a wide variety of natural science and museum specimens for the research staff of the NMNH.
The request for quote (RFQ) and the statement of work (SOW) are provided at the following link. Interested parties need to submit not later than COB April 13th, 2018 to be considered.
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Email: rcburghardt-at-gmail.com Name: Robert C. Burghardt
Organization: Texas A&M University
Title-Subject: [Filtered] Associate Research Scientist - Electron Microscopy
Message: The Image Analysis Laboratory of the College of Veterinary Medicine & Biomedical Sciences at Texas A&M University seeks a highly motivated dedicated individual to work in a state-of-the-art microscopy shared resource.
The individual must have extensive practical expertise in biological sample preparation for transmission electron microscopy of animal tissues and mammalian cell lines. Hands-on knowledge of widefield fluorescence microscopy and confocal and would also be a plus. Excellent verbal and written communication skills and the ability to work with multiple users are essential. A doctoral degree in biology or related discipline is required. A minimum of three years of relevant experience in electron microscopy is required.
Interested individuals should apply for this position via the Texas A&M University website https://tamus.wd1.myworkdayjobs.com/TAMU_External
Position Number: R-003044
Applicants should include a resume along with a description of their practical expertise and contact information for 3 references. Texas A&M University is an Equal Opportunity / Affirmative Action / Veterans / Disability Employer.
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Email: rcburghardt-at-gmail.com Name: Robert C. Burghardt
Organization: Texas A&M University
Title-Subject: [Filtered] Associate Research Scientist - Electron Microscopy
Message: The Image Analysis Laboratory of the College of Veterinary Medicine & Biomedical Sciences at Texas A&M University seeks a highly motivated dedicated individual to work in a state-of-the-art microscopy shared resource.
The individual must have extensive practical expertise in biological sample preparation for transmission electron microscopy of animal tissues and mammalian cell lines. Hands-on knowledge of widefield fluorescence microscopy and confocal and would also be a plus. Excellent verbal and written communication skills and the ability to work with multiple users are essential. A doctoral degree in biology or related discipline is required. A minimum of three years of relevant experience in electron microscopy is required.
Interested individuals should apply for this position via the Texas A&M University website https://tamus.wd1.myworkdayjobs.com/TAMU_External
Position Number: R-003044
Applicants should include a resume along with a description of their practical expertise and contact information for 3 references. Texas A&M University is an Equal Opportunity / Affirmative Action / Veterans / Disability Employer.
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X-from: snkeller-at-techie.com This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both snkeller-at-techie.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: snkeller-at-techie.com Name: Sandra Keller
Organization: TA-Sicco
Title-Subject: [Filtered] TEM time?
Message: Hi All: I am looking to rent time on a TEM in good condition within a few hours of the DFW metroplex as soon as possible. The TEM that we normally use is down at the moment and I am looking for an instrument to use as a backup. I have many years of TEM experience and would require minimal training. Thanks in advance Login Host: 71.8.114.145 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
==============================Original Headers============================== 7, 53 -- From microscopy.listserver-at-gmail.com Mon Mar 12 14:31:56 2018 7, 53 -- Received: from mail-it0-f42.google.com (mail-it0-f42.google.com [209.85.214.42]) 7, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w2CJVuxt001913 7, 53 -- for {microscopy-at-microscopy.com} ; Mon, 12 Mar 2018 14:31:56 -0500 7, 53 -- Received: by mail-it0-f42.google.com with SMTP id k79-v6so13046861ita.2 7, 53 -- for {microscopy-at-microscopy.com} ; Mon, 12 Mar 2018 12:29:43 -0700 (PDT) 7, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 7, 53 -- d=gmail.com; s=20161025; 7, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version 7, 53 -- :in-reply-to:content-language:content-transfer-encoding; 7, 53 -- bh=AVLnXCzyU4/K9b/TKblCyGpdIJ8vCtlXVH0dTWWUF4U=; 7, 53 -- b=bBujQVanSt4cAwqTL5/aU283DhVzk50gRD/d4R0r/Riur5r74bfoVc4t5l+i/UizLf 7, 53 -- PbYWS3yKYX0HTc7/zzt0X6PyvIiXgqfxNXzHOTKwDZHAUW05oaw3EGfz11djz6WJnU2W 7, 53 -- BcGnyL5IxfrClisUzj7DvEYV5aJKC62Pu8n0Ih7Z5FqTfbElLtdbEKWcum9gQ28pq8YN 7, 53 -- +9Kf5Vysj0401IOQPwKlECweFg3fb04nV9Afj4UvD/cHPvncJtqn6LF1ABtEEJCJt4Vp 7, 53 -- 4Wv/IGcWc+KDoyp3iVWTUDniBIVNUBx7ZRfLUe/5Mfx5I7Hatr/PWIJ6C3iXnG23B23K 7, 53 -- X5ZA== 7, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 7, 53 -- d=1e100.net; s=20161025; 7, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date 7, 53 -- :user-agent:mime-version:in-reply-to:content-language 7, 53 -- :content-transfer-encoding; 7, 53 -- bh=AVLnXCzyU4/K9b/TKblCyGpdIJ8vCtlXVH0dTWWUF4U=; 7, 53 -- b=lim+o+3qJNj/1sCUF1YAu96fGl5yoBwuybT5r0wdB+twmV7iCDTLwe3Aa59xXWkm4F 7, 53 -- wnOYW0U/K+qokIw3GQlKz6IQQrg13JIyE2wtBPOAsgVPy02K63rUH8USiJv7KCBg5MC2 7, 53 -- HfVP/Oar7qCSZarFm6y61KlBCk68bfeAaA8bOMA/mOdUKsBD/QjWiw431gnaFOykv67e 7, 53 -- TK0/InQgkDa/EcKCMrDAxmvnFu9WXhcPVAbS+0uLzdlmIf05oqmlPNbOhjJ9ERywyNeV 7, 53 -- Zsq9dXU2pUNRRYPA5GRsIAj++vYkV/BVYbn0VAhVtIyjU8MB6UeukBsdiJoBBUWjSBtR 7, 53 -- 4FMg== 7, 53 -- X-Gm-Message-State: AElRT7FB95v8TCfGzVK2qdwYqs2EvKZxuSPO9Cb6q20DqamqcI4CeR6l 7, 53 -- lglWte15R147zxTRsiUM3RP2/3IM 7, 53 -- X-Google-Smtp-Source: AG47ELuhJvUihxdtQT+d6dRuvN7qROc95rFpWevEo9+FPqXqKMxLJ+fwgGva169phSsp0H1XOft1hg== 7, 53 -- X-Received: by 10.36.33.205 with SMTP id e196mr9711588ita.49.1520882982808; 7, 53 -- Mon, 12 Mar 2018 12:29:42 -0700 (PDT) 7, 53 -- Received: from eduroam062-140.wl.anl-external.org (eduroam062-140.wl.anl-external.org. [130.202.62.140]) 7, 53 -- by smtp.googlemail.com with ESMTPSA id y126sm3976258itc.29.2018.03.12.12.29.42 7, 53 -- for {microscopy-at-microscopy.com} 7, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 7, 53 -- Mon, 12 Mar 2018 12:29:42 -0700 (PDT) 7, 53 -- Subject: viaWWW: time on a TEM in the DFW area 7, 53 -- References: {201803121613.w2CGDBR9021533-at-microscopy.com} 7, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 7, 53 -- From: Microscopy Listserver {microscopy.listserver-at-gmail.com} 7, 53 -- X-Forwarded-Message-Id: {201803121613.w2CGDBR9021533-at-microscopy.com} 7, 53 -- Message-ID: {9d74fa38-21fb-825d-bf42-37565b6f45b8-at-gmail.com} 7, 53 -- Date: Mon, 12 Mar 2018 14:29:42 -0500 7, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) 7, 53 -- Gecko/20100101 Thunderbird/52.6.0 7, 53 -- MIME-Version: 1.0 7, 53 -- In-Reply-To: {201803121613.w2CGDBR9021533-at-microscopy.com} 7, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed 7, 53 -- Content-Language: en-US 7, 53 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: chrisbrantner-at-gwu.edu Name: Chris Brantner
Organization: George Washington University
Title-Subject: [Filtered] Job Opening at George Washington University
Message: The George Washington University Nanofabrication and Imaging Center (GWNIC) is seeking a Laboratory Associate or Technician dependent on experience and qualifications. Duties will include preparation and imaging of biological and materials samples for microscopy from our research labs. Additional duties will include general laboratory management. This is a support position in the GWNIC, for all of the departments of the University and outside users, where we provide high quality microscopy services. The minimum qualifications are as follows: Laboratory Associate: A BA/BS in a related discipline plus 2 years of relevant professional experience or, a Masters degree or higher in a relevant area of study. Degree must be conferred by the start date of the position. If you are interested in this position, apply at: http://www.gwu.jobs/postings/49493. Laboratory Technician: A high school diploma/GED plus 1.5 years of relevant professional experience, or, a Bachelors degree or higher in a relevant area of study. Degree must be conferred by the start date of the position. If you are interested, apply at: http://www.gwu.jobs/postings/49611.
The George Washington University is an Equal Employment Opportunity/Affirmative Action employer that does not unlawfully discriminate in any of its programs or activities on the basis of race, color, religion, sex, national origin, age, disability, veteran status, sexual orientation, gender identity or expression, or on any other basis prohibited by applicable law.
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From davishal182newi-at-gmail.com Tue Mar 13 06:56:38 2018 Return-Path: {davishal182newi-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [182.126.148.246] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id w2DBubco009348 for {microscopylistserverarchive6-at-microscopy.com} ; Tue, 13 Mar 2018 06:56:38 -0500 Received: from unknown (HELO smtp.endend.nl) (Tue, 13 Mar 2018 03:46:00 -0800) by external.newsubdomain.com with SMTP; Tue, 13 Mar 2018 03:46:00 -0800 Received: from mxs.perenter.com [20.85.186.242] by m1.gns.snv.thisdomainl.com with NNFMP; Tue, 13 Mar 2018 03:37:30 -0800 Message-ID: {D029DE67.CD9B27A1-at-gmail.com}
X-from: tobi-at-stanford.edu
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Email: tobi-at-stanford.edu Name: Tobias Beetz
Organization: Stanford University
Title-Subject: [Filtered] Job at Stanford University - Transmission Electron Microscopy Scientist
Message: Transmission Electron Microscopy Scientist, Stanford Nano Shared Facilities The Stanford Nano Shared Facilities (SNSF) is seeking a Transmission Electron Microscopy (TEM) scientist to lead the operations of the facilities FEI Titan Environmental TEM. The TEM scientist will provide training and support to researchers, maintain and optimize the microscope operation, work closely with equipment vendors, maintain and develop training procedures, develop and implement advanced techniques, assist and advice users on specimen preparation and data analysis, provide proof-of-concept service, and engage in research activities. The SNSF Titan ETEM is equipped with a spherical aberration corrector, EELS and EDS, a monochromator and high brightness gun, Lorentz, holography, and tomography capabilities, as well as a suite of in situ holders. S(he) will work to utilize this range of advanced techniques to the highest extent, making them known to the user community, matching appropriate techniques to the individual research projects. S(he) will interact with the broader research community and equipment vendors to be aware of advances in the field, and make recommendations and prepare proposals for future equipment purchases. S(he) will promote and collaborate in the publication and presentation of results and participate in conferences. Stanfords shared nanofacilities offer a comprehensive array of advanced nanofabrication and nanocharacterization tools. Over 1,000 researchers make use of the shared facilities each year in order to further their research programs. The goal of the shared facilities at Stanford University is to provide open, cost-effective access to state-of-the-art nanofabrication and nanocharacterization facilities for scientists and engineers from academia, small and large companies, and government laboratories. The FEI Titan TEM is organized under SNSF in the Electron & Ion Microscopy Suite which currently features a FEI Tecnai TEM as well as two FIB/SEMs and two SEMs. S(he) may supervise student trainers. The TEM scientist will report to the Faculty Director of SNSF.
For more information about SNSF, visit http://snsf.stanford.edu.
Application Deadline: Applications must be submitted by April 30, 2018.
X-from: Taylor, Jordan {J.W.Taylor-at-massey.ac.nz}
Hi
I would think that you could get a lesson on any microtome and be able to use this one - especially if it was one in the Leica family. I learnt on the Ultracut R and didn't need any training to step up to a EM UC 7. I wouldn't limit my learning oppurtunity to just this model of microtome if I were you - they are all essentially the same. Success will come with time and experience.
Maybe if you let the listserver know what problems you were having someone may be able to suggest a work through for you.
Best of luck!
Jordan Taylor Microscopy Technician Manawatu Microscopy and Imaging Centre Massey University Private Bag 11-222 Palmerston North, 4442 Ph +64 6 356 9099 extn 84719
---------------------------------------------------------------------------------------------------- *From:* microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} *Sent:* Saturday, 10 March 2018 6:27:08 a.m. *To:* Taylor, Jordan *Subject:* [Microscopy] viaWWW:Training Inquiry Re: Leica Reichert Ultracut S
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X-from: rvancamp-at-kettering.edu
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Email: rvancamp-at-kettering.edu Name: Rick Van Camp
Organization: Kettering University
Title-Subject: [Filtered] Training Inquiry Re: Leica Reichert Ultracut S
Message: We need to hasten our use of our ultramictrotome. I have identified a few of these still in use but, need more input to find facilities using this instrument on a daily basis that are also willing to train me in its operation. I have made progress on my own yet, have only been able to find one facility willing to provide training. I have also used the manual and made progress yet, I cannot state the manual for this instrument is well written. Finally, it is best for me to locate facilities within the Great Lakes region. I have searched for online training for this and have been unsuccessful.
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I am planning to prepare lung tissue for TEM and Scanning Transmission X-ray Microscopy. An article by Jian Li (2009 Macromolecules 42) A new Approach to Studying Microcapsule Wall Growth Mechanisms stated they used an epoxy resin trimethylolpropane triglycidyl ether (TTE) and 4,4′-methylenebis(2-methylcyclohexylamine) (MBMCA) in a 1:1 weight ratio.
Is anyone familiar with this product? Is there a kit or would one need to purchase the reagents individually from Sigma etc?
Thank you for your suggestions Karen Kelley, UFL
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We were recently examining a box of Amray SEM W filaments and found what we think is an odd feature on some of them. The filament is your basic loop or hairpin filament, but some have a small needle extending from one side of the wire at the apex of the point. The needed would point down the column and has the look of purposeful manufacturing. It's been suggested these are field emission filaments. Our Amray is set up for field emission.
We are having trouble with filament drift in use and short lifetimes but we are reluctant to use these.
I'm open to thoughts.
Stay safe...........
Frank Karl Microscopist Akron Rubber Development Laboratory 2887 Gilchrist Road Akron, Ohio 44305
Oppsss.. Ohh damn! I meant to say our Amray is not set up for field emission.
Sorry about the confusion........ Frank
We were recently examining a box of Amray SEM W filaments and found what we think is an odd feature on some of them. The filament is your basic loop or hairpin filament, but some have a small needle extending from one side of the wire at the apex of the point. The needed would point down the column and has the look of purposeful manufacturing. It's been suggested these are field emission filaments. Our Amray is ( correction add NOT) set up for field emission.
We are having trouble with filament drift in use and short lifetimes but we are reluctant to use these.
I'm open to thoughts.
Stay safe.....
Frank Karl Microscopist Akron Rubber Development Laboratory 2887 Gilchrist Road Akron, Ohio 44305
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a lot of years ago Siemens manufactured for the ELMISKOP TEMs cathodes with this kind of "tip". In German it`s called "Spitzenkathode".
Theoretically it should have some extra luminosity and may deliver - since the beam comes from a very small origin - more coherent electrons. It is known that these tips need a very good vacuum at the cathode, otherwise you will round the tip through ion bombardment or burn the filament very easily.
I suppose it is kind of unstable during heating up and will shift a lot. In my opinion it should be centred and mounted like a LaB6 cathode to bring all the advantages...
Lifetime had been only part of what is known from a "normal" W filament, maybe 10 hours or so...
Best wishes,
Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
It sounds like those are pointed tungsten filaments. In the days of prehistory (aka pre-FEG) in order to get a high brightness/coherence source people used pointed W cathodes. In order to get the tip of the needle hot enough to have reasonable emission, the hairpin ran very hot with the corresponding reduction in lifetime. If I remember, cathode lifetimes of ~10 hours were common. They would have been used for high-resolution SEM or high-coherence TEM imaging.
Cheers, Henk
--------------------
Hendrik O. Colijn Center for Electron Microscopy and AnalysiS The Ohio State University 1305 Kinnear Rd, Suite 100, Columbus, OH 43212
colijn.1-at-osu.edu 614/643-3458 cemas.osu.edu
"Time is that quality of nature which keeps things from happening all at once." (Ray Cummings - 1922) Lately it doesn't seem to be working.
------ Original Message ------ X-from: frank_karl-at-ardl.com To: colijn.1-at-osu.edu Sent: 3/14/2018 12:10:25 PM
I restored an Elmiskop Ia and I have a few boxes of original Spitzenkathode. They give much more coherence -- really impressive! But of course they don't last very long.
Does anyone know of another Elmiskop Ia still in operation?
-- Mike
On 3/14/2018 2:56 PM, diller-at-stefan-diller.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Frank, } } a lot of years ago Siemens manufactured for the ELMISKOP TEMs cathodes with this kind of "tip". In German it`s called } "Spitzenkathode". } } Theoretically it should have some extra luminosity and may deliver - since the beam comes from a very small origin - more } coherent electrons. It is known that these tips need a very good vacuum at the cathode, otherwise you will round the tip through } ion bombardment or burn the filament very easily. } } I suppose it is kind of unstable during heating up and will shift a lot. In my opinion it should be centred and mounted like a } LaB6 cathode to bring all the advantages... } } Lifetime had been only part of what is known from a "normal" W filament, maybe 10 hours or so... } } } Best wishes, } } Stefan } } } ----------------------------------------------------- } Stefan Diller - Scientific Photography } Arndtstrasse 22 } D - 97072 Wuerzburg Germany } ++49-931-7848700 Phone } ++49-931-7848701 Fax } ++49-175-7177051 Mobile } } Websites: } www.nanoflight.info } www.stefan-diller.com } www.electronmicroscopy.info } www.elektronenmikroskopie.info } www.zwillingsprojekt.de } www.assisi.de } Anfahrt: http://Mail.map24.com/Stefan.Diller } ----------------------------------------------------- } } Am 14.03.18 um 16:41 schrieb frank_karl-at-ardl.com: } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } We were recently examining a box of Amray SEM W filaments and found what we think is an odd feature on some of them. The filament is your basic loop or hairpin filament, but some have a small needle extending from one side of the wire at the apex of the point. The needed would point down the column and has the look of purposeful manufacturing. It's been suggested these are field emission filaments. Our Amray is set up for field emission. } } } } We are having trouble with filament drift in use and short lifetimes but we are reluctant to use these. } } } } I'm open to thoughts. } } } } } } } } } } Stay safe........... } } } } Frank Karl } } Microscopist } } Akron Rubber Development Laboratory } } 2887 Gilchrist Road } } Akron, Ohio 44305 } } } } } } } } ==============================Original Headers============================== } } 10, 59 -- From frank_karl-at-ardl.com Wed Mar 14 10:16:24 2018 } } 10, 59 -- Received: from NAM03-CO1-obe.outbound.protection.outlook.com (mail-co1nam03on0057.outbound.protection.outlook.com [104.47.40.57]) } } 10, 59 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w2EFGOA7029396 } } 10, 59 -- for {microscopy-at-microscopy.com} ; Wed, 14 Mar 2018 10:16:24 -0500 } } 10, 59 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } } 10, 59 -- d=ardlcorp.onmicrosoft.com; s=selector1-ardl-com; } } 10, 59 -- h=From:Date:Subject:Message-ID:Content-Type:MIME-Version; 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Applications are invited for Postdoctoral Research Scientist position in the area of thin film deposition and characterization by x-ray diffraction and electron microscopy at Columbia University. The films will be deposited by sputtering in an ultrahigh vacuum deposition chamber. The deposited films will be characterized by X-ray diffraction, scanning electron microscopy conventional and high-resolution transmission and scanning transmission electron microscopy as well as tomographic imaging, analytical electron microscopy and crystal orientation mapping. This is a full-time position and the work will be carried out in the thin film deposition lab, the shared materials characterization and electron microscopy facilities at Columbia University and at the ASRC of the City University of New York. The use of facilities at Brookhaven National Laboratories is also anticipated.
For more details please see http://apam.columbia.edu/katayun-barmak
-- Katayun Barmak Philips Electronics Professor Director, Materials Science and Engineering Program Department of Applied Physics and Applied Mathematics Seeley W. Mudd Building Columbia University 500 West 120th Street, Suite 200, MC 4701 New York, NY 10027-6623 Tel: (212) 854-8267 Fax: (212) 854-8257 Email: katayun.barmak-at-columbia.edu URL: http://apam.columbia.edu/katayun-barmak
==============================Original Headers============================== 4, 56 -- From kb2612-at-columbia.edu Wed Mar 14 17:39:43 2018 4, 56 -- Received: from outprodmail02.cc.columbia.edu (outprodmail02.cc.columbia.edu [128.59.72.51]) 4, 56 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w2EMdhWh027488 4, 56 -- for {Microscopy-at-microscopy.com} ; Wed, 14 Mar 2018 17:39:43 -0500 4, 56 -- Received: from hazelnut (hazelnut.cc.columbia.edu [128.59.213.250]) 4, 56 -- by outprodmail02.cc.columbia.edu (8.14.4/8.14.4) with ESMTP id w2EMXX7f023684 4, 56 -- for {Microscopy-at-microscopy.com} ; Wed, 14 Mar 2018 18:37:36 -0400 4, 56 -- Received: from hazelnut (localhost.localdomain [127.0.0.1]) 4, 56 -- by hazelnut (Postfix) with ESMTP id 3C4A26D 4, 56 -- for {Microscopy-at-microscopy.com} ; Wed, 14 Mar 2018 18:37:38 -0400 (EDT) 4, 56 -- Received: from sendprodmail02.cc.columbia.edu (sendprodmail02.cc.columbia.edu [128.59.72.14]) 4, 56 -- by hazelnut (Postfix) with ESMTP id 1D8656D 4, 56 -- for {Microscopy-at-microscopy.com} ; Wed, 14 Mar 2018 18:37:38 -0400 (EDT) 4, 56 -- Received: from mail-qk0-f198.google.com (mail-qk0-f198.google.com [209.85.220.198]) 4, 56 -- by sendprodmail02.cc.columbia.edu (8.14.4/8.14.4) with ESMTP id w2EMbamD021330 4, 56 -- (version=TLSv1/SSLv3 cipher=AES128-GCM-SHA256 bits=128 verify=NOT) 4, 56 -- for {Microscopy-at-microscopy.com} ; Wed, 14 Mar 2018 18:37:36 -0400 4, 56 -- Received: by mail-qk0-f198.google.com with SMTP id t27so3154530qki.11 4, 56 -- for {Microscopy-at-microscopy.com} ; Wed, 14 Mar 2018 15:37:36 -0700 (PDT) 4, 56 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 56 -- d=1e100.net; s=20161025; 4, 56 -- h=x-gm-message-state:to:from:subject:message-id:date:user-agent 4, 56 -- :mime-version:content-language:content-transfer-encoding; 4, 56 -- bh=1r6S670xqO2Q6F2DYh+zMe7DHwxYfWTKIfPeCSgWkSo=; 4, 56 -- b=BieFnMiD2tHCp3fk38Kx8su7AqBOiIiihovfJAv4lvi6jXhLTo5Ln3IzJWXgnekidI 4, 56 -- bdyVS5Tbh32xuteQYRfpnV9rvFrAQRyHixCxs/ffJrUHUpSp1AfC4x/LmOOQ30E6CC0n 4, 56 -- nEas56oHKstmGzhDI/agCWD5brToPo6PvQTh41l7RPGpxA+gd9nvs5qivXbQCicR4mK7 4, 56 -- 2pTc4wv9clBc+gO4naOMHhQjFhPmoCZPOJn6uV1hrt6J3TiotUD+cc5fizXGtMLts/Oq 4, 56 -- Agy4lsCbXjzaJ9VX+rUZQV8td9IhBPFIIdueTPoLyV2P5Bp/FTsUOSIwYWQHFMjlQtDl 4, 56 -- yfJA== 4, 56 -- X-Gm-Message-State: AElRT7EFLToFiQbxyq3t0z0xL9EsDv7hNQ4+UnW7VOr6dtqfScVotbZU 4, 56 -- V/0RbruIeseII/NnuvcyNM+UEPJscn/D2h6LArcUveZJysGU2lf0/pzfku6IFIfMur92joK6zqZ 4, 56 -- wer3yeLFSqwmeUJKQHZWO4Z5kkDOl 4, 56 -- X-Received: by 10.55.194.77 with SMTP id j13mr9459962qkm.213.1521067056002; 4, 56 -- Wed, 14 Mar 2018 15:37:36 -0700 (PDT) 4, 56 -- X-Google-Smtp-Source: AG47ELthv8+ZkqWZr2k20BHrsT1qQKpPcjw4aC1GgcFWF3MyivrdNc5OgHPLzmJJpMes7LXUqYbaGA== 4, 56 -- X-Received: by 10.55.194.77 with SMTP id j13mr9459944qkm.213.1521067055801; 4, 56 -- Wed, 14 Mar 2018 15:37:35 -0700 (PDT) 4, 56 -- Received: from [192.168.1.4] (ool-457c292f.dyn.optonline.net. [69.124.41.47]) 4, 56 -- by smtp.gmail.com with ESMTPSA id d198sm2345796qkg.91.2018.03.14.15.37.34 4, 56 -- for {Microscopy-at-microscopy.com} 4, 56 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 4, 56 -- Wed, 14 Mar 2018 15:37:35 -0700 (PDT) 4, 56 -- To: Microscopy-at-microscopy.com 4, 56 -- From: "Barmak K." {kb2612-at-columbia.edu} 4, 56 -- Subject: Post Doctoral Position at Columbia University 4, 56 -- Message-ID: {47b12b52-350c-955f-43d7-4b8faac0f25d-at-columbia.edu} 4, 56 -- Date: Wed, 14 Mar 2018 18:37:33 -0400 4, 56 -- User-Agent: Mozilla/5.0 (Windows NT 10.0; WOW64; rv:52.0) Gecko/20100101 4, 56 -- Thunderbird/52.6.0 4, 56 -- MIME-Version: 1.0 4, 56 -- Content-Type: text/plain; charset=utf-8; format=flowed 4, 56 -- Content-Language: en-US 4, 56 -- Content-Transfer-Encoding: 7bit 4, 56 -- X-No-Spam-Score: Local 4, 56 -- X-Scanned-By: MIMEDefang 2.78 on 128.59.72.14 ==============================End of - Headers==============================
I did own an Elmiskop II and an Elmiskop 101 in the 80ies and 90ies.
Still I have a lot of special gaskets and o-rings, aperture holders and documents on these TEMs. Also tools etc...
If you or anybody else out there reviving an old SIEMENS machine needs parts, contact me ;-)
And: I still have some of the "Spitzenkathodes" and a lot of the normal ones.
Best wishes,
Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Am 14.03.18 um 21:10 schrieb mike.marko.em-at-gmail.com: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Listers, } } I restored an Elmiskop Ia and I have a few boxes of original } Spitzenkathode. They give much more coherence -- really impressive! } But of course they don't last very long. } } Does anyone know of another Elmiskop Ia still in operation? } } -- Mike } } } On 3/14/2018 2:56 PM, diller-at-stefan-diller.com wrote: } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Hi Frank, } } } } a lot of years ago Siemens manufactured for the ELMISKOP TEMs cathodes with this kind of "tip". In German it`s called } } "Spitzenkathode". } } } } Theoretically it should have some extra luminosity and may deliver - since the beam comes from a very small origin - more } } coherent electrons. It is known that these tips need a very good vacuum at the cathode, otherwise you will round the tip through } } ion bombardment or burn the filament very easily. } } } } I suppose it is kind of unstable during heating up and will shift a lot. In my opinion it should be centred and mounted like a } } LaB6 cathode to bring all the advantages... } } } } Lifetime had been only part of what is known from a "normal" W filament, maybe 10 hours or so... } } } } } } Best wishes, } } } } Stefan } } } } } } ----------------------------------------------------- } } Stefan Diller - Scientific Photography } } Arndtstrasse 22 } } D - 97072 Wuerzburg Germany } } ++49-931-7848700 Phone } } ++49-931-7848701 Fax } } ++49-175-7177051 Mobile } } } } Websites: } } www.nanoflight.info } } www.stefan-diller.com } } www.electronmicroscopy.info } } www.elektronenmikroskopie.info } } www.zwillingsprojekt.de } } www.assisi.de } } Anfahrt: http://Mail.map24.com/Stefan.Diller } } ----------------------------------------------------- } } } } Am 14.03.18 um 16:41 schrieb frank_karl-at-ardl.com: } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } } } We were recently examining a box of Amray SEM W filaments and found what we think is an odd feature on some of them. The filament is your basic loop or hairpin filament, but some have a small needle extending from one side of the wire at the apex of the point. The needed would point down the column and has the look of purposeful manufacturing. It's been suggested these are field emission filaments. Our Amray is set up for field emission. } } } } } } We are having trouble with filament drift in use and short lifetimes but we are reluctant to use these. } } } } } } I'm open to thoughts. } } } } } } } } } } } } } } } Stay safe........... } } } } } } Frank Karl } } } Microscopist } } } Akron Rubber Development Laboratory } } } 2887 Gilchrist Road } } } Akron, Ohio 44305 } } } } } } } } } } } } ==============================Original Headers============================== } } } 10, 59 -- From frank_karl-at-ardl.com Wed Mar 14 10:16:24 2018 } } } 10, 59 -- Received: from NAM03-CO1-obe.outbound.protection.outlook.com (mail-co1nam03on0057.outbound.protection.outlook.com [104.47.40.57]) } } } 10, 59 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w2EFGOA7029396 } } } 10, 59 -- for {microscopy-at-microscopy.com} ; Wed, 14 Mar 2018 10:16:24 -0500 } } } 10, 59 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } } } 10, 59 -- d=ardlcorp.onmicrosoft.com; s=selector1-ardl-com; } } } 10, 59 -- h=From:Date:Subject:Message-ID:Content-Type:MIME-Version; } } } 10, 59 -- bh=2PcwAjeSNN8RC97C84vpLFpcZxaCbOkdde7toMTPHyA=; } } } 10, 59 -- b=T9YFEVJ3QWX3SVbCpFi1UiXjwcIc3LIdx1UaaHyt6tpkciMgNmQ0GqE/keYb4faapaU8y4VjGxX9ruRdhbhKNTb/TrKsoaHFhiQNFaeSpR9pXghve7osHSXINGGRCwQXkX7c7Cc5sOPImz/ZoWG3OuRzPOyITrhJfO04DxFSPKE= } } } 10, 59 -- Received: from DM5PR15MB1452.namprd15.prod.outlook.com (