Welcome to the another year of operation of the Microscopy ListServer a free service to the world wide microscopy community, sponsored jointly by your Friendly Neighborhood SysOp and the Microscopy Society of America.
During 2016, the ListServer delivered your messages to more than 4200 subscribers around the world, with minimal hassels (that I know about). For those of you that are statistics junkies this year you generated ~ 171+ Gbits of Email traffic and ~ 2.1 Million Email messages were sent out this year by my tired and old little server. Down abit from previous years, but still a steady flow.
This year I will be migrating the Listserver to a new server and ISP configuration so you might see a short outages during testing and transistions.
As usual you don't want to know how much Junk Mail and spam has been filtered out far more than you might expect. Apologies to those that have problems with my filters.
The complete Microscopy ListServer Archives for 2016-1994 (~ are on-line at
http://www.microscopy.com.
A couple of IMPORTANT reminders:
If you leave on vacation/holiday use the on-line form to UNSUBSCRIBE your Email address from the listserver. The out-of-office / on-vacation autoreply messages are a real nuisance to posters.
Do not reply to message with the return address of:
MicroscopyListserver-noreply-at-microscopy.com
these are messages forwarded usually from the WWW posting form. They do not go back to the poster but rather into a black hole, which I rarely check. If you see a message that has this "No-Reply" return address please post your reply/comment/answer to:
Microscopy-at-microscopy.com
or if you wish to reply privately, look at the username in the body of the message the originators Email address is usually listed therein.
As always if you have questions about suitability of postings or are having problems, feel free to contact me at (zaluzec-at-microscopy.com)
Cheers,
Nestor Your Friendly Neighborhood SysOp
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
Microscopy-at-Microscopy.com
============================================
==============================Original Headers============================== 22, 49 -- From microscopy.listserver-at-gmail.com Sun Jan 1 16:27:06 2017 22, 49 -- Received: from mail-it0-f50.google.com (mail-it0-f50.google.com [209.85.214.50]) 22, 49 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v01MR68u027431 22, 49 -- for {microscopy-at-microscopy.com} ; Sun, 1 Jan 2017 16:27:06 -0600 22, 49 -- Received: by mail-it0-f50.google.com with SMTP id o141so246261447itc.0 22, 49 -- for {microscopy-at-microscopy.com} ; Sun, 01 Jan 2017 14:31:18 -0800 (PST) 22, 49 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 22, 49 -- d=gmail.com; s=20161025; 22, 49 -- h=from:reply-to:to:subject:message-id:date:user-agent:mime-version 22, 49 -- :content-transfer-encoding; 22, 49 -- bh=NNpxxPO+w4u4DEi4Fz7tekCRHMgUElz1BqxjAwj8Fcw=; 22, 49 -- b=K8G9WwO1q9O2PU4wh3r468lAxrDu5LsMvcOQRFxexFcVuKCnCJPFgOji1MhLp4zeqM 22, 49 -- pVCmOoKRTkmF6MkvBw87/0xQnoFnVCzSsX7v4SDQVQloA5YKfO02HL2g/G1NrL1VZ0W/ 22, 49 -- k+tWT/a9Xue7Qhe1LLdCJokJ5PkGpfXKyIPuZRTDqP8ytMFewKeqbmmqWovdL5YIlI/T 22, 49 -- gvCVbx9xX9xP119L6EiDsshLV210xkRfVUzD0xmVpGI1LhXZ7M5C9V6Bb016OPBOKgNG 22, 49 -- o5w3RIiZ5uMdclov/BUD8FJrpgPgjspVyBplLGPuqH8g17KH1pTxgFXHK81d1/pf6B5z 22, 49 -- iRIQ== 22, 49 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 22, 49 -- d=1e100.net; s=20161025; 22, 49 -- h=x-gm-message-state:from:reply-to:to:subject:message-id:date 22, 49 -- :user-agent:mime-version:content-transfer-encoding; 22, 49 -- bh=NNpxxPO+w4u4DEi4Fz7tekCRHMgUElz1BqxjAwj8Fcw=; 22, 49 -- b=IPMGEcy5uEY+SrZRP7raSP7yjdNh26ultUIWcDUF9a1vuXXTYngg23iuwDeF18oZTL 22, 49 -- Krk1wL/LYPIUpcDdQShRUa+FNm7U0F/U3+nBqZ5n5xdvkxNTh4jlovKww6oWm2E7Kc7A 22, 49 -- VWeaoQOxEKLrTNy6QG2zGmHae0RFPv3F8wtqp5BSl0fb2UEeO8MQYunzBbu1YpwaRYYz 22, 49 -- Cw2YWokCeESTW/cvtM0Bk+d1XnpgaPtNWk6JfeTnsKTYMXnjJ9wE9/0SRX6x3P03Gkoq 22, 49 -- RTgfTQ56Fns/zzKCRLybXO2jMl+0rB0OwalIPFu2ERRwEDiUKzDtppmeQyMGonB3yGMh 22, 49 -- +9DQ== 22, 49 -- X-Gm-Message-State: AIkVDXLXPhJ1YYExKpYizUQ5IS9P3FSjKpWQSZpSvYtUQN2hUX6OKrRdsGbbwjjqouSTDQ== 22, 49 -- X-Received: by 10.36.8.5 with SMTP id 5mr45966946itc.40.1483309878271; 22, 49 -- Sun, 01 Jan 2017 14:31:18 -0800 (PST) 22, 49 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:b9a1:15b:ee63:f3bc]) 22, 49 -- by smtp.googlemail.com with ESMTPSA id p20sm29795554itc.2.2017.01.01.14.31.17 22, 49 -- for {microscopy-at-microscopy.com} 22, 49 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 22, 49 -- Sun, 01 Jan 2017 14:31:17 -0800 (PST) 22, 49 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 22, 49 -- X-Google-Original-From: MicroscopyListserver-NoReply 22, 49 -- {microscopylistserver-noreply-at-microscopy.com} 22, 49 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 22, 49 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 22, 49 -- Subject: Administrivia: Happy New Year 2017 22, 49 -- Message-ID: {69c0b85a-a9f5-4207-6511-e3f05a3b0a59-at-microscopy.com} 22, 49 -- Date: Sun, 1 Jan 2017 16:31:16 -0600 22, 49 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:45.0) 22, 49 -- Gecko/20100101 Thunderbird/45.6.0 22, 49 -- MIME-Version: 1.0 22, 49 -- Content-Type: text/plain; charset=utf-8; format=flowed 22, 49 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Does this go to archives now that I have fixed the forwarding system???
=========================================== Dr. Nestor J. Zaluzec Argonne National Laboratory Photon Sciences Division 9700 S. Cass Ave Bldg 212 / A-143 Argonne, Illinois 60439 USA Email: Zaluzec-at-aaem.amc.anl.gov
Tel: 530-NES-TORZ (530-637-8679) has Voice Mail Lab: 630-252-7901 Fax: 630-252-4798
Senior Scientist - Argonne National Laboratory Fellow of the Microscopy Society of America Senior Fellow the Computational Institute - University of Chicago E.P. Wigner Fellow - Oak Ridge National Laboratory Past President Microscopy Society of America Adjunct Professor of Physics - Northern Illinois University & the University of Illinois at Chicago Visiting Professor of Microscopy - Manchester University
Well my worst nightmares were realized on Monday Night (Jan 2) when I upgraded my server. Lots of my finely tuned filters and spam killers "broke". As such the Listserver has been down for ~ 2 days.
I believe all is fixed now. If you find/identify a problem please let me know.
Cheers,
Nestor Your Friendly Neighborhood SysOp
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Hi all,
Happy New Year!
Does anyone have a manual for a Sorvall Porter-Blum microtome, Type JB-4? If yes, can you please send me a copy?
best regards,
Beth Richardson
Georgia Electron Microscopy Lab
University of Georgia
Athens, GA
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both sbernstein-at-mail.sdsu.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: EM facility director position at SDSU
Message: Job Announcement: Electron Microscope Facility Lead (Instructional Support Technician III), College of Sciences - Department of Biology
Job ID:6232; San Diego State University Main Campus; Full-Time Regular
Please note: A completed application is required in order to be considered for this position.
About SDSU: San Diego State University is the oldest and largest higher education institution in the San Diego region. Since its founding in 1897, SDSU has grown to offer bachelor's degrees in 91 areas, master's degrees in 78 areas and doctorates in 22 areas. SDSU's approximately 35,000 students participate in an academic curriculum distinguished by direct contact with faculty and an increasing international emphasis that prepares them for a global future.
SDSU is a large, diverse, urban university and Hispanic-Serving Institution with a commitment to diversity, equity and inclusive excellence. Our campus community is diverse in many ways, including in terms of race, religion, color, sex, age, disability, marital status, sexual orientation, gender identity and expression, national origin, pregnancy, medical condition, and covered veteran status. We strive to build and sustain a welcoming environment for all. SDSU is an equal opportunity employer. SDSU is seeking applicants with demonstrated experience and/or commitment to teaching and working effectively with individuals from diverse backgrounds and members of underrepresented groups.
Position Information
Full-time, permanent (probationary) position. The incumbent is responsible for the day-to-day management of the SDSU Electron Microscope Facility, oversight of Biology common-use/shared research microscopes (other than EMs), collaborate with and train users of Biology research microscopes, keep EM Facility and Biology research microscope equipment funded and operational, order supplies, maintain EM Facility website. The incumbent also provides input and guidance on the microscopic needs of Biology lab courses.
Salary Range: Anticipated Hiring Salary Range: $4,107 - $6,473 per month (CSU Classification Salary Range: $4,107 - $6,667 per month). The competitive salary is determined by the education, experience, and qualifications the candidate brings to the position, internal equity, and the hiring department's fiscal resources.
Responsibilities: Training Biology and other SDSU faculty, staff and students in the theory and operation of EM Facility equipment and other Biology research microscopes Consulting with Biology and other SDSU faculty and students on research projects using light microscopy, transmission electron microscopy, and scanning electron microscopy Oversee external users of the facility and manage the fee-for-service accounting Collaborating and sharing expertise with Biology faculty and staff to incorporate EM Facility equipment and other Departmental microscope resources into the Biology curriculum Maintain equipment associated with EM Facility and Biology research microscopes, coordinate with repair providers; routine servicing of light microscopes used in biology courses Acquiring additional instrumentation to enlarge or improve the capabilities of the EM Facility and Biology research microscopes. This includes submitting grants, or assisting others in the preparation of grants, to obtain funds for new or updated instrumentation. Expanding the menu of microscopic techniques the Facility offers, through training and self-study Maintaining appropriate purchasing procedures, and tracking and billing users of facility Establishing outreach efforts to area schools and to the community, including designing and publishing the Facility Web site Representing the EM Facility and SDSU through service within professional organizations
Knowledge, Skills & Abilities: Knowledge of the principles and methods related to performing support services. Knowledge of the principles, information, methods and techniques related to discipline to which assigned. Knowledge of the materials and supplies related to the curriculum, their characteristics, and uses. Ability to plan, organize and schedule work; ability to operate and repair technical and scientific equipment. Ability to coordinate support service to meet a comprehensive variety of needs. Ability to develop off-campus resources related to the discipline for obtaining materials or equipment. Experience and Education
Equivalent to four years of experience providing instructional support services for a related unit or discipline, or in producing materials or supplies or repairing equipment in a discipline related to specialty area to which assigned.
OR
Equivalent to two years of college with 16 semester units in courses involving extensive use of materials, supplies, or equipment and in a discipline related to the area to which assigned may be substituted for one year of the required experience.
OR
Equivalent to four years of college with 16 semester units in courses involving extensive use of materials, supplies, or equipment and in a discipline related to the specialty area to which assigned may be substituted for two years of the required experience.
Specialized Requirements: Knowledge of the principles and methods related to light and electron microscopy Knowledge of the principles, information, methods and techniques related to biological sciences. Ability to coordinate support service to meet a comprehensive variety of needs of users. Ability to develop off-campus resources related to the operation, maintenance, and upgrading of Electron Microscopy Facility equipment Preferred Qualifications
Training/experience in operational design and theory of confocal/epifluorescent microscopy, light microscopy, scanning and transmission electron microscopy Experience in preparing samples for confocal/light microscopy, scanning and transmission electron microscopy Training/experience operating confocal/light microscopy, scanning and transmission electron microscopy Experience collecting and processing digital images Experience purchasing scientific equipment Relevant Masters degree Ph.D. in biological science Proven abilities in TEM and SEM operations as well as sample preparation techniques for these microscopes In addition to knowledge of the principles, information, methods and techniques related to biological sciences, it is preferable that individual also has knowledge in electron microscopy use in other scientific and engineering fields.
Application Procedures: Review of applications will begin on Wednesday, January 31, 2017; position will remain open until filled. The on-line application should be completed in detail. COMPLETION OF THE ONLINE APPLICATION IS REQUIRED FOR CONSIDERATION, A RESUME ALONE WILL NOT SUFFICE. Website for applying through the PeopleSoft system: https://cmsweb.cms.sdsu.edu/psp/HSDPRDF/EMPLOYEE/HRMS/c/HRS_HRAM.HRS_CE.GBL?Page=HRS_CE_HM_PRE&Action=A&SiteId=1
Login Host: 146.244.235.159 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
As you start this new year, a reminder that this year's run of Analytical and Quantitative Light Microscopy, held at the Marine Biological Laboratory, Woods Hole, MA, USA, will run May 3 - May 12, 2017. Applications are due January 27, 2017.
The application is accessible via the course web site is at http://www.mbl.edu/aqlm. Financial assistance is available.
AQLM is a comprehensive and intensive course in light microscopy for researchers in biology, medicine, and material sciences. This course provides a systematic and in-depth examination of the theory of image formation and application of digital methods for exploring subtle interactions between light and the specimen. This course emphasizes the quantitative issues that are critical to the proper interpretation of images obtained with modern wide-field, confocal microscopes and new emerging technologies.
Laboratory exercises, demonstrations, and discussions include: • geometrical and physical optics of microscope image formation including Abbe's theory of the microscope and Fourier optics; • phase contrast, polarization and interference microscopy; • fluorescence microscopy, quantification of fluorescence, and fluorescent proteins; • principles and application of digital imaging and quantitative digital image deconvolution; • digital image processing and object identification and tracking; • live cell and ratiometric imaging for FRET and ion concentration imaging; • confocal microscopy and specialized methods such as TIRF and FLIM; and • new advances in light microscopy such as FCS, PALM, STORM, SIM and Light Sheet.
Within the course, we often refer to AQLM as "Microscopy Boot Camp". We cover every topic with a lecture and an in-depth hands-on lab. It's intense, it's hardcore, and it's really fun.
Why not join us for AQLM (or recommend one of your lab members or core facility users)? You'll never forget it.
Directors: Justin Taraska (National Institutes of Health), Jagesh Shah (Harvard Medical School) Course Laboratory Director: Wendy Salmon (Whitehead Institute/MIT)
Again, applications due January 27, 2017 and can be access through course web site at http://www.mbl.edu/aqlm.
Cheers, Jagesh, Justin and Wendy
Visit our Facebook page: http://www.facebook.com/aqlmcourse Follow us on Twitter: -at-BeadsinMay
Wendy
~~~~~~~~~~~~~~~~~~~~~~~ Wendy Salmon Light Microscopy Specialist Whitehead Institute for Biomedical Research W.M. Keck Imaging Facility **STREET ADDRESS CHANGE** 455 Main St, Rm 447 Cambridge, MA 02142 c: 617-429-0158 e: wsalmon-at-wi.mit.edu w: http://staffa.wi.mit.edu/microscopy/
==============================Original Headers============================== 16, 41 -- From wsalmon-at-wi.mit.edu Thu Jan 5 07:08:11 2017 16, 41 -- Received: from ptolemy.wi.mit.edu (ptolemy.wi.mit.edu [18.4.1.120]) 16, 41 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v05D8BfU027039 16, 41 -- for {microscopy-at-microscopy.com} ; Thu, 5 Jan 2017 07:08:11 -0600 16, 41 -- X-IronPort-AV: E=Sophos;i="5.33,321,1477972800"; 16, 41 -- d="scan'208";a="11308408" 16, 41 -- Received: from unknown (HELO mars.wi.mit.edu) ([10.9.4.20]) 16, 41 -- by ptolemy.wi.mit.edu with ESMTP; 05 Jan 2017 08:12:34 -0500 16, 41 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 16, 41 -- by mars.wi.mit.edu (Postfix) with ESMTP id C0BA431EDCE 16, 41 -- for {microscopy-at-microscopy.com} ; Thu, 5 Jan 2017 08:12:34 -0500 (EST) 16, 41 -- Received: from mars.wi.mit.edu ([127.0.0.1]) 16, 41 -- by localhost (mars.wi.mit.edu [127.0.0.1]) (amavisd-new, port 10032) 16, 41 -- with ESMTP id 2_j5h33ilqTW for {microscopy-at-microscopy.com} ; 16, 41 -- Thu, 5 Jan 2017 08:12:34 -0500 (EST) 16, 41 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 16, 41 -- by mars.wi.mit.edu (Postfix) with ESMTP id 3C06D31EDD2 16, 41 -- for {microscopy-at-microscopy.com} ; Thu, 5 Jan 2017 08:12:34 -0500 (EST) 16, 41 -- X-Virus-Scanned: amavisd-new at mars.wi.mit.edu 16, 41 -- Received: from mars.wi.mit.edu ([127.0.0.1]) 16, 41 -- by localhost (mars.wi.mit.edu [127.0.0.1]) (amavisd-new, port 10026) 16, 41 -- with ESMTP id hR_rowKutR4r for {microscopy-at-microscopy.com} ; 16, 41 -- Thu, 5 Jan 2017 08:12:34 -0500 (EST) 16, 41 -- Received: from io.wi.mit.edu (io.wi.mit.edu [10.9.4.21]) 16, 41 -- by mars.wi.mit.edu (Postfix) with ESMTP id 2250B31EDCE 16, 41 -- for {microscopy-at-microscopy.com} ; Thu, 5 Jan 2017 08:12:34 -0500 (EST) 16, 41 -- Date: Thu, 5 Jan 2017 08:12:33 -0500 (EST) 16, 41 -- From: Wendy Salmon {wsalmon-at-wi.mit.edu} 16, 41 -- To: microscopy {microscopy-at-microscopy.com} 16, 41 -- Message-ID: {2076351021.11730088.1483621953838.JavaMail.zimbra-at-wi.mit.edu} 16, 41 -- In-Reply-To: {1563420676.11456151.1483569237344.JavaMail.zimbra-at-wi.mit.edu} 16, 41 -- References: {1563420676.11456151.1483569237344.JavaMail.zimbra-at-wi.mit.edu} 16, 41 -- Subject: 2nd Announcement: Analytical and Quantitative Light Microscopy 2017 16, 41 -- MIME-Version: 1.0 16, 41 -- Content-Type: text/plain; charset=utf-8 16, 41 -- X-Originating-IP: [10.9.41.56] 16, 41 -- X-Mailer: Zimbra 8.6.0_GA_1191 (ZimbraWebClient - GC55 (Mac)/8.6.0_GA_1191) 16, 41 -- Thread-Topic: 2nd Announcement: Analytical and Quantitative Light Microscopy 2017 16, 41 -- Thread-Index: YkApMdUfqeWYfhn8ozABZ9snK8fNPH2VKVPL 16, 41 -- Content-Transfer-Encoding: 8bit 16, 41 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v05D8BfU027039 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ravi.thakkar369-at-gmail.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: ravi.thakkar369-at-gmail.com Name: Ravi
Title-Subject: [Filtered] EDS report shows Boron, even its Boron peak is not present in spectrum.
Message: Hi Listeners, I am using Oxford EDS (SiLi) on Hitachi S 3500N SEM. Surprisingly, I am getting false Boron atomic% count.(some times values are in negative) in spectrum report without any detectable peak in the spectrum. Here I am using Inca 5.05 I am not able to remove the Boron from the element count table by confirm elements options because absence of boron peak.
Login Host: 129.130.145.107 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both gary-at-microtechnics.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: gary-at-microtechnics.com Name: Dr Gary Gaugler
Organization: Microtechnics
Title-Subject: [Filtered] BSD for Zeiss Supra 40
Message: Hi All:
Does anyone know of BSD options (scintillator or YAG) for the Zeiss Supra 40? Robinson has retired. A standard Deben Centaurus does not fit a chamber port without a large extra cost.
Any ideas or suggestions are appreciated.
gary g.
Login Host: 104.220.26.145 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ravi.thakkar369-at-gmail.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: ravi.thakkar369-at-gmail.com Name: Rav
Title-Subject: [Microscopy] viaWWW: EDS report shows Boron, even its Boron peak is not present.
Message: I am having boron counts even its absent in confirm elements. Here, I am using Oxford EDS on Hitachi SEM using Inca 5.05 Hope that image file linked to following link will helpful to you to understand the issue.
At AgResearch, NZ's Government owned agricultural research institute, we have two microscopy related positions with closing dates in the next few weeks (27th Jan for scientist position, and 20th Jan for PhD scholarship).
These should be of interest to people interested the structure/biology of hair and wool (hard-keratin materials).
The scientist position is aimed at an early career scientist who has completed a PhD and has good team skill with a track record in innovative science.
The PhD scholarship focuses on location of hair-specific proteins within the developing fibre using high-pressure freezing and immunolabelling.
Full details of the two positions can be found on AgResearch's website (www.agresearch.co.nz/careers) and there are also links from AgResearch's Linked In jobs page.
Duane
____________________________ Dr Duane P Harland Senior Scientist T +64 3 321 8710 E duane.harland-at-agresearch.co.nz AgResearch Limited Lincoln Research Centre Cnr Springs Rd & Gerald Street, Lincoln Private Bag 4749 Christchurch 8140, New Zealand T +64 3 325 9900 F +64 3 325 9946 www.agresearch.co.nz ======================================================================= Attention: The information contained in this message and/or attachments from AgResearch Limited is intended only for the persons or entities to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipients is prohibited by AgResearch Limited. If you have received this message in error, please notify the sender immediately. =======================================================================
==============================Original Headers============================== 9, 37 -- From Duane.Harland-at-agresearch.co.nz Sun Jan 8 15:31:37 2017 9, 37 -- Received: from out1103.nz.smxemail.com (out1103.nz.smxemail.com [203.84.134.34]) 9, 37 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v08LVaww010502 9, 37 -- for {microscopy-at-microscopy.com} ; Sun, 8 Jan 2017 15:31:36 -0600 9, 37 -- DKIM-Signature: v=1; a=rsa-sha256; d=nz.smxemail.com; s=alpha; c=relaxed/relaxed; 9, 37 -- q=dns/txt; i=-at-nz.smxemail.com; t=1483911371; 9, 37 -- h=From:Sender:Reply-To:Subject:Date:Message-ID:To:Cc; 9, 37 -- bh=RC1+BGNpD3idI0P9C8PWETtzsGO4AvHoIP2vg/yZqZ0=; 9, 37 -- b=htfz+2+qoNdPKgBlgVEXSxDWcnWGJyeKxr0zYfF1E6QYdTk5KomQLo4EcymU1nWh 9, 37 -- tJnBdZzY8eEYvd8Cx/2zEOKmL5QsAnRZs1sKebcDsLdyL2a9Z1exCdSWxT7Ze2hU 9, 37 -- ApMBA9qNUOqrIYEUatWeQd0Njyf1QLSYtiPpKWFZbVs=; 9, 37 -- Received: from invexcpv02.agresearch.co.nz ([202.20.102.50]) 9, 37 -- by omr.nz.smxemail.com with ESMTP (using TLSv1 9, 37 -- with cipher AES128-SHA (128/128 bits)) 9, 37 -- id 5872B0CB-135A0D6A-at-mta1104.omr; 9, 37 -- Sun, 08 Jan 2017 21:36:11 +0000 9, 37 -- Received: from INVEXCPV01.agresearch.co.nz ([147.158.128.23]) by 9, 37 -- invexcpv02.agresearch.co.nz ([147.158.130.77]) with mapi id 14.03.0294.000; 9, 37 -- Mon, 9 Jan 2017 10:36:07 +1300 9, 37 -- From: "Harland, Duane" {Duane.Harland-at-agresearch.co.nz} 9, 37 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 9, 37 -- Subject: EM scientist and PhD scholarship on immunolabelling TEM in New 9, 37 -- Zealand 9, 37 -- Thread-Topic: EM scientist and PhD scholarship on immunolabelling TEM in New 9, 37 -- Zealand 9, 37 -- Thread-Index: AQHSafWipn+d/TiqikWdZ6kPnec4rA== 9, 37 -- Date: Sun, 8 Jan 2017 21:36:07 +0000 9, 37 -- Message-ID: {C1CB2BCA7689064DA55A366147C8BEF1013DB42233-at-invexcpv01.agresearch.co.nz} 9, 37 -- Accept-Language: en-GB, en-NZ, en-US 9, 37 -- Content-Language: en-GB 9, 37 -- X-MS-Has-Attach: 9, 37 -- X-MS-TNEF-Correlator: 9, 37 -- x-originating-ip: [202.20.103.209] 9, 37 -- Content-Type: text/plain; charset="iso-8859-1" 9, 37 -- MIME-Version: 1.0 9, 37 -- Content-Transfer-Encoding: 8bit 9, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v08LVaww010502 ==============================End of - Headers==============================
From Unique.VisitsCheap-at-mg-style.cn Mon Jan 9 19:44:54 2017 Return-Path: {Unique.VisitsCheap-at-mg-style.cn} Received: from mg-style.cn ([106.249.239.99]) by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0A1ioW6031075 for {microscopylistserverarchive6-at-microscopy.com} ; Mon, 9 Jan 2017 19:44:52 -0600 DKIM-Signature: v=1; a=rsa-sha1; c=relaxed/relaxed; d=mg-style.cn; s=key79; h=Reply-To:From:To:Subject:MIME-Version: Content-Type:Content-Transfer-Encoding; bh=louU+QpRetqUJT80roctj W+Gn+w=; b=CxaQltowR1iQA0fezWRkAILZ4qrvkgfkr7D0wPvyfEAKQHxUpyjls uEb/wjr53f/esOGs2/bKkxRqd3XCLrT26aG1wt9nLFHjHA6B43R/DXLn2DLAySt3 icPRe4dR5dDRPNxiXaE1tgX7jXQLrFDfwdJs/jweOtXZfJoa1hVTkY= Reply-To: Unique.VisitsCheap-at-mg-style.cn charset="utf-8" Content-Transfer-Encoding: 8bit X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v0A1ioW6031075
hello Here it is http://www.mg-style.cn/detail.php?id=112
Unsubscribe option available on the footer of our website
by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0ACwP6k028397 for {MicroscopyListserverArchive6-at-microscopy.com} ; Tue, 10 Jan 2017 06:58:25 -0600 Received: (from mail-at-localhost) by microscopy.com (8.12.11.20060308/8.12.11/Submit) id v0ACwPxp028395; Tue, 10 Jan 2017 06:58:25 -0600
X-from: m.aindow-at-uconn.edu
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both m.aindow-at-uconn.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: m.aindow-at-uconn.edu Name: Mark Aindow
Organization: University of Connecticut
Title-Subject: [Filtered] Postdoctoral Positions in the UConn-FEI Center for Advanced Microscopy and Materials Analysis
Message: The University of Connecticut (UConn) has partnered with FEI to establish the Center for Advanced Microscopy and Materials Analysis (CAMMA), which includes a suite of seven new electron- and ion-beam instruments. CAMMA will form part of the state-of-the-art Advanced Characterization Laboratories in the UConn Technology Park, which is currently under construction and will open later this year. Five of the new instruments are currently housed in the Institute of Materials Science at UConn and will be relocated to the new Technology Park facility later in 2017. There are two CAMMA postdoctoral positions available immediately: 1. 3D studies of complex microstructures using Xe plasma FIB. This position will involve the development and exploitation of serial sectioning and data reconstruction strategies using the FEI Helios plasma FIB. Candidates should have a good working knowledge of FIB and electron microscopy techniques, including EDXS and EBSD. A clear understanding of microstructural issues in metallic alloys, ceramics and coatings is important for this post. 2. Microstructural development in aerospace alloys. This position will involve working with local aerospace industries on a range of projects relating to microstructural issues in Ni, Ti and Al alloys, and in thermal barrier coatings. Candidates should have extensive experience with relevant materials systems and with a broad range of electron microscopy techniques. Due to the nature of some of the projects, only US citizens or permanent residents will be considered for this second position.
Both of these positions are for one year in the first instance, with the possibility of renewal. To apply, please send a resume, together with a full list of publications, a statement of relevant experience, and contact details for three references to Prof. Mark Aindow (m.aindow-at-uconn.edu). The University of Connecticuts commitment to excellence is complemented by our commitment to building a culturally diverse community. We encourage applications from under-represented groups, including minorities, women, and people with disabilities. The University of Connecticut is an equal opportunity employer and program provider. Screening of applications will begin immediately and will continue until the positions are filled.
Login Host: 71.235.254.247 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
Microscopy-at-Microscopy.com
============================================
==============================Original Headers============================== 15, 53 -- From microscopy.listserver-at-gmail.com Tue Jan 10 06:58:24 2017 15, 53 -- Received: from mail-it0-f53.google.com (mail-it0-f53.google.com [209.85.214.53]) 15, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0ACwOWp028392 15, 53 -- for {microscopy-at-microscopy.com} ; Tue, 10 Jan 2017 06:58:24 -0600 15, 53 -- Received: by mail-it0-f53.google.com with SMTP id r185so47080182ita.0 15, 53 -- for {microscopy-at-microscopy.com} ; Tue, 10 Jan 2017 05:03:07 -0800 (PST) 15, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 15, 53 -- d=gmail.com; s=20161025; 15, 53 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 15, 53 -- :mime-version:in-reply-to:content-transfer-encoding; 15, 53 -- bh=F+JPI9Ce9vlRdlVSKJtHkmxIRfUiNYQOBouhhP0te8A=; 15, 53 -- b=Tj10h11SWFvV3tTWS7rB66q2IcPHcWU2rAIpAyzgy4vVFrDPyiSOMIz6UgpxuaJna3 15, 53 -- 9eC5K+bbQ3Q2fzvnxMty7k+ApJxfEDTeXEQ17axA6M6xgN26bGjRGQsFp8iRJPOPv0jY 15, 53 -- f9RxUWqojEu8pezGZSRHC/iyOtPcdFdlLSJxfsXVs6vItt4kMX0G6wmL1P1cenlppmsW 15, 53 -- QgKDIcCSLrw/pMH8FmLDrQ6lW8AyBT6RDIAMkZdlrAzmRBoYdBMElrahCcQAu8BohAZy 15, 53 -- dVSnfwIZYDMqEomIDoHZ5l/dGg4Tz1RlaRafpTJLq4gD/XGhdnXxOC2v3E7dxBWjVUqB 15, 53 -- Z6ww== 15, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 15, 53 -- d=1e100.net; s=20161025; 15, 53 -- h=x-gm-message-state:from:subject:references:to:reply-to:message-id 15, 53 -- :date:user-agent:mime-version:in-reply-to:content-transfer-encoding; 15, 53 -- bh=F+JPI9Ce9vlRdlVSKJtHkmxIRfUiNYQOBouhhP0te8A=; 15, 53 -- b=pc/3iiefJ3MaiPGoaKG7cjT7G3VTYHO070t/8iSVc+BKWEuFDHg2lnKzMDRfobCKmF 15, 53 -- FaEsh0YF5QxopIdlpKkN+holatS9rbP/zdVnf4mqCJWvYF5qEqcs4vR1gmblAvLLl1fH 15, 53 -- 3EJY/P1t5/Oga/S6AHEkyXlGOv42O4tEfbNGhJideNp9SOkX0Bva1QLt2EY2Lb92AMrQ 15, 53 -- dy4UE297G1B1RtLyueJS1GkKID7FszPo8AtfdYE+vN1RdxIy5rt73VaKJs2TNHMBgYIX 15, 53 -- oQ2ifn8cXHkGqJ3HcNlCMXzY3t7u/NYvhicBmHpnKucWPdRHFhxnyNoq/QEdnSfeOvd/ 15, 53 -- Mj/Q== 15, 53 -- X-Gm-Message-State: AIkVDXLA/orD7I7NCp74viodG18SScyfamB4Bh3Or5Eu7w/1PYzkZ70i9ljbKl/aqY5UDg== 15, 53 -- X-Received: by 10.36.3.84 with SMTP id e81mr2950249ite.115.1484053386488; 15, 53 -- Tue, 10 Jan 2017 05:03:06 -0800 (PST) 15, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:b015:d90b:b94f:8554]) 15, 53 -- by smtp.googlemail.com with ESMTPSA id g186sm1211850itb.21.2017.01.10.05.03.05 15, 53 -- for {microscopy-at-microscopy.com} 15, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 15, 53 -- Tue, 10 Jan 2017 05:03:05 -0800 (PST) 15, 53 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 15, 53 -- X-Google-Original-From: MicroscopyListserver-NoReply 15, 53 -- {microscopylistserver-noreply-at-microscopy.com} 15, 53 -- Subject: viaWWW:Postdoctoral Positions in the UConn-FEI Center for Advanced 15, 53 -- Microscopy and Materials Analysis 15, 53 -- References: {201701101138.v0ABc7bB025133-at-microscopy.com} 15, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 15, 53 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 15, 53 -- X-Forwarded-Message-Id: {201701101138.v0ABc7bB025133-at-microscopy.com} 15, 53 -- Message-ID: {af45e71e-d08b-1988-211a-6ec7431e52e0-at-microscopy.com} 15, 53 -- Date: Tue, 10 Jan 2017 07:03:04 -0600 15, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:45.0) 15, 53 -- Gecko/20100101 Thunderbird/45.6.0 15, 53 -- MIME-Version: 1.0 15, 53 -- In-Reply-To: {201701101138.v0ABc7bB025133-at-microscopy.com} 15, 53 -- Content-Type: text/plain; charset=UTF-8; format=flowed 15, 53 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
From steven.345345.f-at-gmail.com Tue Jan 10 11:19:33 2017 Return-Path: {steven.345345.f-at-gmail.com} Received: from gmail.com ([106.249.239.99]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v0AHJUvT029050 for {microscopylistserverarchive6-at-microscopy.com} ; Tue, 10 Jan 2017 11:19:32 -0600 Message-ID: {6C886BB8.CC131669-at-gmail.com}
X-from: vakimler-at-oakland.edu
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both vakimler-at-oakland.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Organization: Oakland University Eye Research Institute
Title-Subject: [Filtered] LR White Ultrathin Sections Prepared for ImmunoGold
Message: How long (in terms of days) in advance can I cut ultrathin sections (usually 70-90 nm) in preparation for immunogold applications? The resin was medium hardness with catalyst, and I polymerized it in a 55 degree oven overnight.
I was wondering if the surface of the ultrathin section would be altered over a few days after cutting and antibodies would be somewhat hindered from access to epitopes.
Login Host: 141.210.132.220 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
*********************************************************************************** Forwarded from "Ask a Microscopist" Please remember that the person asking the question is likely not a member the listserver, and **any reply should go directly to the poster** as well as to the list. Using the "reply" function in your email does *not* send your answer to the person asking the question. Please copy their email address from their question. *********************************************************************************** Ask a Microscopist
The following Ask a Microscopist form submission was received from your website.
Name: Jacar Brocker School: Delft University of Technology Grade/Education Level: Graduate Location: Delft, NL Email: j.h.brocker-at-student.tudelft.nl
X-from: jpshield-at-uga.edu
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jpshield-at-uga.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: jpshield-at-uga.edu Name: John Shields
Organization: University of Georgia
Title-Subject: [Filtered] Bio TEM Workshop -
Message: Biological TEM Workshop
This intensive, three-day workshop will provide a practical and basic theoretical introduction to the Transmission Electron Microscope and biological sample preparation techniques. Each day will consist of lecture, discussion and hands-on training led by GEM staff. What: Anyone requiring training on TEM and biological sample preparation. The workshop will be limited to 6 participants based on the availability of equipment. When: Monday through Wednesday, March 8-10, 2017, 8am-5pm each day (lunch is provided)
Where: 115 D.E. Brooks Drive, 154 Barrow Hall, University of Georgia, Athens, GA 30602
Registration: Contact John Shields (jpshield-at-uga.edu) for more information and to sign up. Registration requires iLab account through the GEM website. https://uga.ilabsolutions.com/account/login
Deadline: February 27, 2017
Login Host: 198.137.20.67 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Ursel.Bangert-at-ul.ie as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: Ursel.Bangert-at-ul.ie Name: Ursel Bangert
Organization: University of Limerick
Title-Subject: [Filtered] Invitation to submit abstracts to the Celtic Sessions at mmc2017
Message: Microcscience Microscopy Congress 2017 Celtic Sessions
The Microscopy Society of Ireland (MSI) is co-organising two sessions (together with the Scottish Microscopy Group, SMG) at the Microscience Microscopy Congress (mmc2017), organised by the Royal Microscopical Society. The event will take place in Manchester, UK, from July 3rd-6th. The international mmc2017 conference will comprise over 30 symposia, with excellent speakers and vibrant supporting poster sessions. Further to presenting new research in Ireland and Scotland, the Celtic Sessions are aimed at showcasing and encouraging collaborative research, and all members of the international microscopy community are sincerely invited to contribute to the Celtic Sessions, which will include invited and regular talks, as well as flash presentations and poster sessions. The abstract submission for mmc2017 is now open. We are inviting abstracts for the Celtic Sessions: Biological Processes at the Nanoscale Inorganic Nanomaterials For more information about how to participate and submit abstracts, please go to the MSI website http://microscopy.ie/ or http://microscopy.ie/2017.php, or submit your abstract directly through the mmc website http://mmc-series.org.uk/about/news/abstract-submission-now-open or through the official mmc2017 flyer http://microscopy.ie/Doc/MSI_Call_for_Papers_Flyer2.pdf.
Login Host: 86.41.249.14 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
} Dear sir/madam, For a masters course we have to find the market size for a planar positioning stage. We are having trouble finding sales numbers or estimating how many microscopists use an automatic positioning stage with their microscope or how many would like to use one if the price is right. Hopefully you could provide us with some global estimates. Kind regards, Jacar Brocker (4079450) }
Global estimates?
Does asking someone else to do the research work for one's degree allow one to earn it, I wonder?
And a Masters in marketing at that.
Ask A Microscopist may be a reasonable start, though asking around the University would probably work even better.
Find someone who may use or be interested in product and talk with them. When you have learned what you and they want to learn, ask if they know someone else with whom you can talk. Repeat the above until you have learned enough.
No number of degrees, doctorates or whatever will avoid that. If you want to succeed at sales, marketing, engineering, science and a number of other disciplines, you must ignore the knot in your stomach, ignore the fear of failure, embarrassment or humiliation, get out there and talk to people. In practice, most of the time there will not be failure, embarrassment or humiliation. Generally, people will be pleased you take an interest. For many people, a coffee, croissant and 20 minute break from work makes a nice change, too.
An ex colleague of mine used sometimes to come back from meetings with potential or actual customers, suppliers, partners or whatever and use the term "WoFT". If one meets with people in those circumstances it is for each party to learn, even if what one learns is that nobody wants the product. IMHO, the only party to whom "WoFT" can apply is oneself.
Gordon.
==============================Original Headers============================== 13, 20 -- From gordon-at-gscott.co.uk Thu Jan 12 04:52:39 2017 13, 20 -- Received: from osmia.gscott.co.uk (87-127-155-63.static.enta.net [87.127.155.63]) 13, 20 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0CAqc5k027778 13, 20 -- for {microscopy-at-microscopy.com} ; Thu, 12 Jan 2017 04:52:39 -0600 13, 20 -- Received: from [10.0.0.101] (CoolLED-PC.gscott.co.uk [10.0.0.101]) 13, 20 -- by osmia.gscott.co.uk (Postfix) with ESMTPS id 9CCFBDF9EA; 13, 20 -- Thu, 12 Jan 2017 10:57:00 +0000 (GMT) 13, 20 -- Subject: Re: [Microscopy] Ask a Microscopist - marketing question re: planar 13, 20 -- positioning stages 13, 20 -- References: {201701112208.v0BM8bSj015503-at-microscopy.com} 13, 20 -- To: microscopy-at-microscopy.com, j.h.brocker-at-student.tudelft.nl 13, 20 -- From: Gordon Scott {gordon-at-gscott.co.uk} 13, 20 -- Message-ID: {522dd9b3-bfb3-9cd4-441d-7836eccd4e46-at-gscott.co.uk} 13, 20 -- Date: Thu, 12 Jan 2017 10:57:00 +0000 13, 20 -- User-Agent: Mozilla/5.0 (X11; Linux x86_64; rv:45.0) Gecko/20100101 13, 20 -- Thunderbird/45.5.1 13, 20 -- MIME-Version: 1.0 13, 20 -- In-Reply-To: {201701112208.v0BM8bSj015503-at-microscopy.com} 13, 20 -- Content-Type: text/plain; charset=windows-1252; format=flowed 13, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
From conversion.seo-at-gmail.com Thu Jan 12 06:26:21 2017 Return-Path: {conversion.seo-at-gmail.com} Received: from gmail.com ([106.249.239.99]) by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0CCQHPP006958 for {microscopylistserverarchive6-at-microscopy.com} ; Thu, 12 Jan 2017 06:26:19 -0600 Reply-To: conversion.seo-at-gmail.com charset="utf-8" Content-Transfer-Encoding: 8bit X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v0CCQHPP006958
Hello Quality Social Services to improve web presence http://www.conversion-seo.cn/social-media-packages/
Unsubscribe Option available on the footer of our website
by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0DKhRpR009936 for {MicroscopyListserverArchive6-at-microscopy.com} ; Fri, 13 Jan 2017 14:43:28 -0600 Received: (from mail-at-localhost) by microscopy.com (8.12.11.20060308/8.12.11/Submit) id v0DKhRQA009934; Fri, 13 Jan 2017 14:43:27 -0600
Organization: Florida State University
Title-Subject: Postdoc position available
Job Title: Post-Doctoral Associate - Electron Microscopist
Summary Description:
Florida State University (FSU) and National High Magnetic Field Laboratory (NHMFL) invite applications for a post-doctoral research associate position to undertake research projects led by Prof. Fumitake Kametani. The research projects aim to understand the nano- and atomic-structural correlations to the material properties by using the advanced electron microscopes including aberration-corrected atomic resolution (scanning) transmission electron microscope (S/TEM) and focused ion beam (FIB). A person who has an experience in the atomic and nano-structural analyses of complicated materials such as high temperature superconductors, carbon nanotube composites, perovskite thin films or energy-related materials is encouraged to apply. This position requires expertise in atomic resolution imaging in a TEM/STEM and nano-fabrication by using a FIB. As listed below, we already have the state-of-art electron microscopes here, so he/she is expected to start working material problems right away.
This position is a full time position with an anticipated starting salary at a minimum of $48,000 and includes health benefits.
Major microscope-related instruments at FSU and NHMFL: * JEOL ARM200cF Cs-corrected S/TEM with Electron Energy Loss Spectroscopy (EELS) and Energy-dispersive X-ray Spectroscopy (EDS) * Carl Zeiss 1540EsB field emission scanning electron microscope and FIB equipped with Gas Injection System and OmniProbe in-situ lift-out tool * Gatan PIPS ion mill * Micro Support Axis Pro SS ex-situ lift-out tool * JEOL IB-19500 CP ion cross section polisher
Requirements: * Ph.D. in materials science, physics, engineering, chemistry, or related field. * Must have experience to operate TEM. * Must have experience preparing specimens using FIB. * Preferred to have experience operating Cs-corrected STEMs. * Preferred to have experience in EELS.
Please directly send CV including 2 references to:
Fumitake Kametani Associate Professor Department of Mechanical Engineering, Florida State University, and Applied Superconductivity Center, National High Magnetic Field Laboratory 2031 E. Paul Dirac Dr., Tallahassee, FL 32310 Tel: (850) 645-7491 E-mail: fkametani-at-fsu.edu
==============================Original Headers============================== 11, 52 -- From fkametani-at-fsu.edu Fri Jan 13 14:43:27 2017 11, 52 -- Received: from NAM01-BY2-obe.outbound.protection.outlook.com (mail-by2nam01on0064.outbound.protection.outlook.com [104.47.34.64]) 11, 52 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0DKhRQl009931 11, 52 -- for {Microscopy-at-Microscopy.Com} ; Fri, 13 Jan 2017 14:43:27 -0600 11, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=fsu.onmicrosoft.com; 11, 52 -- s=selector1-fsu-edu; 11, 52 -- h=From:Date:Subject:Message-ID:Content-Type:MIME-Version; 11, 52 -- bh=i1laWG3U0gEI+mcQq38ao7n9eVzgUyRWrAu08pA0K8E=; 11, 52 -- b=JMbSM8wMO1lnQjNZnEg9K1NrMe9kB8PtMOH0qY52bDdXTJPXTVV54mJuhgjNCMwllaDX1g2dkDL2gykoLXFkSYvJmF3Rf/UsUlKTN9vINcMRcK6+8MEHmG8HGEKKSDi0grhZmvXYNnsGfDCDCX1RIH+WM6qd/yQB+mqVlh5OCUw= 11, 52 -- Received: from DM2PR02MB526.namprd02.prod.outlook.com (10.141.54.156) by 11, 52 -- DM2PR02MB528.namprd02.prod.outlook.com (10.141.54.28) with Microsoft SMTP 11, 52 -- Server (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_256_CBC_SHA384_P384) id 11, 52 -- 15.1.829.7; Fri, 13 Jan 2017 20:47:52 +0000 11, 52 -- Received: from DM2PR02MB526.namprd02.prod.outlook.com ([10.141.54.156]) by 11, 52 -- DM2PR02MB526.namprd02.prod.outlook.com ([10.141.54.156]) with mapi id 11, 52 -- 15.01.0829.019; Fri, 13 Jan 2017 20:47:52 +0000 11, 52 -- From: "Kametani, Fumitake" {fkametani-at-fsu.edu} 11, 52 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com} 11, 52 -- Subject: Postdoc position available 11, 52 -- Thread-Topic: Postdoc position available 11, 52 -- Thread-Index: AdJt3ivWkIk72qWcQnyx5AAy2OqSig== 11, 52 -- Date: Fri, 13 Jan 2017 20:47:52 +0000 11, 52 -- Message-ID: {DM2PR02MB52673FBAAE2ABF729D3D2BEB6780-at-DM2PR02MB526.namprd02.prod.outlook.com} 11, 52 -- Accept-Language: en-US 11, 52 -- Content-Language: en-US 11, 52 -- X-MS-Has-Attach: 11, 52 -- X-MS-TNEF-Correlator: 11, 52 -- authentication-results: spf=none (sender IP is ) 11, 52 -- smtp.mailfrom=fkametani-at-fsu.edu; 11, 52 -- x-originating-ip: [144.174.179.73] 11, 52 -- x-ms-office365-filtering-correlation-id: 74490038-c248-4514-8f58-08d43bf5717a 11, 52 -- x-microsoft-antispam: UriScan:;BCL:0;PCL:0;RULEID:(22001);SRVR:DM2PR02MB528; 11, 52 -- x-microsoft-exchange-diagnostics: 1;DM2PR02MB528;7:tQ8sVNNVk1XBxcFEXVGLMYjH7KvSG//kuqM32dLKwDkNOapqIBbAjavRcz25cUgQ9g7WN61Kaj34U6H3BuFwSxZLIR+To4iyrucWjunwlISrZnEOcy2jSBh5tuoXZA5b878V3mdGiVdArov0Bxeqawz2OFCa3inZ/QnyTgd9/dVraibtDDsYn3/7dGQzgpEPW0t6N2r/an+QjYXb6eyaXaHfdh/MonhBfvQrwXbUdy+ioBZA7D1E0oiPm1BjHqZka8geBA1igCcWA2E8m3DRCmJz3hPnMbcsgNGsqtO5Uuk6SATfSNvtbPTZPlOuy79Aj7aGEaXCIAJBQKto7QY2uYSHw+CRGdsoGL6FJd7yMTTn6jKLAPj8Tknz7CSSDVhZMJ68JEpgu2wm7NAOuCeSFexWcFOPhOrbi0r8U3cD//HwzincXOU+sc5p2+wf+rkxsYb/ec1r+IlEEMu3iYH4GQ== 11, 52 -- x-microsoft-antispam-prvs: {DM2PR02MB528C8B740D59734D93F856FB6780-at-DM2PR02MB528.namprd02.prod.outlook.com} 11, 52 -- x-exchange-antispam-report-test: UriScan:(129945304726314)(278428928389397)(5213294742642); 11, 52 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:(6040375)(601004)(2401047)(5005006)(8121501046)(3002001)(10201501046)(6041248)(20161123562025)(20161123555025)(20161123564025)(20161123560025)(6072148);SRVR:DM2PR02MB528;BCL:0;PCL:0;RULEID:;SRVR:DM2PR02MB528; 11, 52 -- x-forefront-prvs: 018632C080 11, 52 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10009020)(6009001)(7916002)(39450400003)(50944005)(252514010)(199003)(45994003)(189002)(107886002)(8676002)(81156014)(81166006)(189998001)(97736004)(8936002)(3480700004)(77096006)(6436002)(38730400001)(3660700001)(55016002)(5640700003)(99286003)(2501003)(88552002)(3280700002)(25786008)(6506006)(86362001)(9686003)(68736007)(7116003)(33656002)(5660300001)(110136003)(102836003)(50986999)(2906002)(66066001)(3846002)(2900100001)(6116002)(54356999)(75432002)(101416001)(74316002)(450100001)(105586002)(6916009)(2351001)(7736002)(122556002)(305945005)(7696004)(92566002)(106356001)(27001);DIR:OUT;SFP:1101;SCL:1;SRVR:DM2PR02MB528;H:DM2PR02MB526.namprd02.prod.outlook.com;FPR:;SPF:None;PTR:InfoNoRecords;A:1;MX:1;LANG:en; 11, 52 -- received-spf: None (protection.outlook.com: fsu.edu does not designate 11, 52 -- permitted sender hosts) 11, 52 -- spamdiagnosticoutput: 1:99 11, 52 -- spamdiagnosticmetadata: NSPM 11, 52 -- Content-Type: text/plain; charset="us-ascii" 11, 52 -- MIME-Version: 1.0 11, 52 -- X-OriginatorOrg: fsu.edu 11, 52 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 13 Jan 2017 20:47:52.1715 11, 52 -- (UTC) 11, 52 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted 11, 52 -- X-MS-Exchange-CrossTenant-id: a36450eb-db06-42a7-8d1b-026719f701e3 11, 52 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: DM2PR02MB528 11, 52 -- Content-Transfer-Encoding: 8bit 11, 52 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v0DKhRQl009931 ==============================End of - Headers==============================
From rosaronald70-at-gmail.com Sat Jan 14 23:53:38 2017 Return-Path: {rosaronald70-at-gmail.com} Received: from gmail.com ([183.111.66.21]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v0F5rZW6022865 for {microscopylistserverarchive6-at-microscopy.com} ; Sat, 14 Jan 2017 23:53:36 -0600 Message-ID: {EF2986AB.D798EF4F-at-gmail.com}
X-from: vakimler-at-oakland.edu
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both vakimler-at-oakland.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] LR White Ultrathin Sections Prepared for ImmunoGold
Message: Hello, Second Request - Not sure if the first message was transmitted... What is the maximum time I need to wait in order to apply immunogold reagents (buffers, etc.) to ultrathin sections of LR White blocks (Medium hardness with catalyst? Thanks much, Vickie
Login Host: 141.210.132.220 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
I do not think there is such a thing as a maximum time you have to wait before which LR white sections would be suited for immuno. Perhaps you are wondering if there is a minimum time before sections are suited? As far as our experience goes LR-white and Lowicryl sections seem to keep pretty much indefinitely. Perhaps it would be wise if they were kept in a dessicator. I do not think there is a minimum time one should consider having to wait. If things change post-sectioning I would expect epitopes to change (oxidize?) and that hardly ever is beneficial. And how would ‘buffers etc’ relate to that, I do not understand?
Are you getting results, or not getting results, that make you wonder whether section age plays a role?
If you like, please feel free to get in touch off-list. I will be happy to help.
Jan Leunissen - - - Aurion - ImmunoGold Reagents www.aurion.nl
==============================Original Headers============================== 9, 35 -- From leunissen-at-aurion.nl Mon Jan 16 18:20:18 2017 9, 35 -- Received: from nm11-vm2.access.bullet.mail.bf1.yahoo.com (nm11-vm2.access.bullet.mail.bf1.yahoo.com [216.109.114.225]) 9, 35 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0H0KH0V020760 9, 35 -- for {microscopy-at-microscopy.com} ; Mon, 16 Jan 2017 18:20:18 -0600 9, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1484612696; bh=6gxrqluLWgQ7XyCrQJ7BDd0QDfMuuFqjUzs7pf/1nxE=; h=Subject:From:In-Reply-To:Date:Cc:References:To:From:Subject; b=kMdPBGVy6G+HOV9lPFVb4PgI48Yaf1zMZs/qhVJlhe1mYsj8SgGrv4R+9VFWhxTl4AXTntPEEyuX7jcLbMZDz0uBKcyknpEt0eqTTeJqqi04gD/Y5oMmdUE2SkEG10xPAP8ydlaMFThXqkGxWxilnXTOdSCxB497xPPtiiW2Uagsgl8jK05bcIejeYoVBrq+a2Ur5WQ4wZk3r12QScSPcyaXI93FeJBroLyQEAZLD7Lez8C0IT1j5MtA/p859/AcUqMf5Qr6Z628vrVEkP/1GxZUhTjUlkICXmZnQD6/2JFf/K2K82pbz3w9EqhHONkCxt5zrU4jjiiEQ6mahRAYdA== 9, 35 -- Received: from [66.196.81.159] by nm11.access.bullet.mail.bf1.yahoo.com with NNFMP; 17 Jan 2017 00:24:56 -0000 9, 35 -- Received: from [98.138.226.240] by tm5.access.bullet.mail.bf1.yahoo.com with NNFMP; 17 Jan 2017 00:24:56 -0000 9, 35 -- Received: from [127.0.0.1] by smtp111.sbc.mail.ne1.yahoo.com with NNFMP; 17 Jan 2017 00:24:55 -0000 9, 35 -- X-Yahoo-Newman-Id: 942867.29936.bm-at-smtp111.sbc.mail.ne1.yahoo.com 9, 35 -- X-Yahoo-Newman-Property: ymail-3 9, 35 -- X-YMail-OSG: iqgaVIAVM1ldH49kVDcXX4XZXLCeGJtjQATeJsFWXvO.JBY 9, 35 -- 1SWioi7Ux9IAYWBNZqP1eW.VEzqa9y1fQOkwZ9xiBnTAmwD6RFFzwZtXT9dp 9, 35 -- sxi1eOwiR5d32oC5pOMSvBq_Prb_xPw89Nhrr7VfQNPyD2UHaJg36KHtS1OT 9, 35 -- FTz5NwM9mNIGgQM0cihMy1QYy9ERz6b8R5LNYIWS729LnB0KtkqBvqqkLnQs 9, 35 -- AxUvAfTXvBAegyZDlSjf_pXwyZGUSX0.dK6rH9luGqD_agTJF69X33dZH.lU 9, 35 -- 5r4jN9cSEGwX.6D7uHdsm040PfzEEF.4IgZNLsAYkWsTdDeEJznGmrdbEQZi 9, 35 -- fr8mq3wxmmBpyZgLo6Zzy0TMYphcd0q8vgF3hSvhiDzaD0rtmQQUXIsrr8h8 9, 35 -- 6DZgZiQqwzwOpPRmiHjrLV1IviBUxx0rm9S810tg_LCdvHbaP5YXJXNTe9lo 9, 35 -- _mcoVFPwgbuJM_GTbyV2aYhxaUqbe4sU4lbT7t_7BwMSaQQDRmcUnhgvh0Ul 9, 35 -- LpYRd7Rykj9X2noDo3y_QbI6ozWBMxccf55limj0e8UW9WKCmemPgYkhDaDa 9, 35 -- 1I6uBWazirBg1Pw-- 9, 35 -- X-Yahoo-SMTP: E1TINkOswBCOniPaNnp23qBGQpLN4KsU.cr0KdlJlWhF 9, 35 -- Content-Type: text/plain; charset=utf-8 9, 35 -- Mime-Version: 1.0 (Mac OS X Mail 9.3 \(3124\)) 9, 35 -- Subject: Re: [Microscopy] viaWWW:LR White Ultrathin Sections Prepared for ImmunoGold 9, 35 -- From: Jan Leunissen {leunissen-at-aurion.nl} 9, 35 -- In-Reply-To: {201701162359.v0GNxklD002178-at-microscopy.com} 9, 35 -- Date: Tue, 17 Jan 2017 13:24:49 +1300 9, 35 -- Cc: microscopy-at-microscopy.com 9, 35 -- Message-Id: {50900377-4A4C-4BB3-9AF6-608B6A1B2058-at-aurion.nl} 9, 35 -- References: {201701162359.v0GNxklD002178-at-microscopy.com} 9, 35 -- To: vakimler-at-oakland.edu 9, 35 -- X-Mailer: Apple Mail (2.3124) 9, 35 -- Content-Transfer-Encoding: 8bit 9, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v0H0KH0V020760 ==============================End of - Headers==============================
Here's a story: Someone once had LR-White sections that were more than two years old, from another institution. They went ahead and applied their antibody, secondary antibody and gold, and seemed to have every expectation that it would work (whether based on experience or blind faith, I do not know). However, they needed images immediately for a grant proposal, or at least they needed to know if a particular protein was on a particular organ in the squid. My TEM (the only one in the state) was down. In a panic, we stuck them in the SEM wehere we could see the gold particles on the surface of the section, and we could also see outlines of the ultrastructure because parts of the tissue had bulged out of the resin enough to give some relief. In fact, it looked really cool. And they were able to state that the protein was in that organ. If you've ever sectioned a block and then let it sit for some time (hours, years?), and than sectioned again you've probably noticed that some tissues expand out further than others over time.
Anyway, in this case I'm sure their antibody would have worked right away on freshly-cut sections as well as on sections that had been sitting on grids for more than two years. The resin may flow somewhat over time. Your mileage may vary. Perform a bunch of timing experiments and report back!
Aloha, Tina
} Email: vakimler-at-oakland.edu Name: Vickie Kimler } } Organization: Eye Research Institute } } Title-Subject: [Filtered] LR White Ultrathin Sections Prepared for ImmunoGold } } Message: Hello, } Second Request - Not sure if the first message was transmitted... } What is the maximum time I need to wait in order to apply immunogold reagents } (buffers, etc.) to } ultrathin sections of LR White blocks (Medium hardness with catalyst? } Thanks much, } Vickie }
==============================Original Headers============================== 6, 21 -- From tina-at-pbrc.hawaii.edu Mon Jan 16 19:27:16 2017 6, 21 -- Received: from b1000.pbrc.hawaii.edu (b1000.pbrc.hawaii.edu [128.171.22.30]) 6, 21 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0H1RFg6016186 6, 21 -- for {Microscopy-at-microscopy.com} ; Mon, 16 Jan 2017 19:27:16 -0600 6, 21 -- Received: from b1000.pbrc.hawaii.edu (localhost [127.0.0.1]) 6, 21 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id v0H1VqXn012379 6, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 6, 21 -- for {Microscopy-at-microscopy.com} ; Mon, 16 Jan 2017 15:31:53 -1000 (HST) 6, 21 -- Received: from localhost (tina-at-localhost) 6, 21 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP id v0H1Vqft012375 6, 21 -- for {Microscopy-at-microscopy.com} ; Mon, 16 Jan 2017 15:31:52 -1000 (HST) 6, 21 -- X-Authentication-Warning: b1000.pbrc.hawaii.edu: tina owned process doing -bs 6, 21 -- Date: Mon, 16 Jan 2017 15:31:52 -1000 (HST) 6, 21 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 6, 21 -- X-X-Sender: tina-at-b1000 6, 21 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 6, 21 -- Subject: Re: [Microscopy] viaWWW:LR White Ultrathin Sections Prepared for 6, 21 -- ImmunoGold 6, 21 -- Message-ID: {Pine.GSO.4.64.1701161529260.12266-at-b1000} 6, 21 -- MIME-Version: 1.0 6, 21 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
Hello, I wanted to know if anyone has had experience using curtain wall systems to segment a space for microscopes without building hard walls. I want to make a somewhat-temporary home for an instrument. Laser safety curtaining is common but way too expensive and unnecessary, since we have no lasers. Has anyone used any of the industrial-type curtain systems to divide up a space? Thanks for any information.
Regards, Larry Scipioni ZS Genetics
==============================Original Headers============================== 3, 52 -- From les-at-zsgenetics.com Tue Jan 17 13:27:06 2017 3, 52 -- Received: from gateway21.websitewelcome.com (gateway21.websitewelcome.com [192.185.45.155]) 3, 52 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0HJR5Mm018712 3, 52 -- for {microscopy-at-microscopy.com} ; Tue, 17 Jan 2017 13:27:06 -0600 3, 52 -- Received: from cm3.websitewelcome.com (unknown [108.167.139.23]) 3, 52 -- by gateway21.websitewelcome.com (Postfix) with ESMTP id 3EC58400D9595 3, 52 -- for {microscopy-at-microscopy.com} ; Tue, 17 Jan 2017 13:31:45 -0600 (CST) 3, 52 -- Received: from gator2013.hostgator.com ([50.87.144.13]) 3, 52 -- by cm3.websitewelcome.com with 3, 52 -- id ZXXj1u00y0HZY1u01XXkjj; Tue, 17 Jan 2017 13:31:45 -0600 3, 52 -- DKIM-Signature: v=1; a=rsa-sha256; q=dns/txt; c=relaxed/relaxed; 3, 52 -- d=zsgenetics.com; s=default; h=Content-Transfer-Encoding:Content-Type: 3, 52 -- MIME-Version:Message-ID:Date:Subject:To:From:Sender:Reply-To:Cc:Content-ID: 3, 52 -- Content-Description:Resent-Date:Resent-From:Resent-Sender:Resent-To:Resent-Cc 3, 52 -- :Resent-Message-ID:In-Reply-To:References:List-Id:List-Help:List-Unsubscribe: 3, 52 -- List-Subscribe:List-Post:List-Owner:List-Archive; 3, 52 -- bh=w1HMFSQ3qjgKsehRQSv57Jy5dMB1VmzALc2p8oqZTzI=; b=mM2CBSECySlrLyx65O6NEROl40 3, 52 -- xlUFwWR0RFfRGCO1lE9WexzfrjiaOYpqtYp9VLI/KVf11VpcvmN/8NdAUz66Q9H376bm4cm41EFol 3, 52 -- oe+oqQ9bn9Iqv1MUjZAA9be0gTdfGIilVRE4PBAh6KewVtKF6rO0ZxXXwZO3Z2KZi+BA=; 3, 52 -- Received: from zsgenetics03.z.subnet.rcn.com ([146.115.5.114]:50951 helo=LarryPC) 3, 52 -- by gator2013.hostgator.com with esmtpsa (TLSv1.2:ECDHE-RSA-AES256-GCM-SHA384:256) 3, 52 -- (Exim 4.87) 3, 52 -- (envelope-from {les-at-zsgenetics.com} ) 3, 52 -- id 1cTZTr-000KOX-3Z 3, 52 -- for microscopy-at-microscopy.com; Tue, 17 Jan 2017 13:31:43 -0600 3, 52 -- From: {les-at-zsgenetics.com} 3, 52 -- To: {microscopy-at-microscopy.com} 3, 52 -- Subject: curtain systems 3, 52 -- Date: Tue, 17 Jan 2017 14:31:41 -0500 3, 52 -- Message-ID: {042501d270f8$5522a5a0$ff67f0e0$-at-zsgenetics.com} 3, 52 -- MIME-Version: 1.0 3, 52 -- Content-Type: text/plain; 3, 52 -- charset="us-ascii" 3, 52 -- Content-Transfer-Encoding: 7bit 3, 52 -- X-Mailer: Microsoft Outlook 16.0 3, 52 -- Thread-Index: AdJw9zrv+yTN6KMwQkegivozoJS38w== 3, 52 -- Content-Language: en-us 3, 52 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report 3, 52 -- X-AntiAbuse: Primary Hostname - gator2013.hostgator.com 3, 52 -- X-AntiAbuse: Original Domain - microscopy.com 3, 52 -- X-AntiAbuse: Originator/Caller UID/GID - [47 12] / [47 12] 3, 52 -- X-AntiAbuse: Sender Address Domain - zsgenetics.com 3, 52 -- X-BWhitelist: no 3, 52 -- X-Source-IP: 146.115.5.114 3, 52 -- X-Exim-ID: 1cTZTr-000KOX-3Z 3, 52 -- X-Source: 3, 52 -- X-Source-Args: 3, 52 -- X-Source-Dir: 3, 52 -- X-Source-Sender: zsgenetics03.z.subnet.rcn.com (LarryPC) [146.115.5.114]:50951 3, 52 -- X-Source-Auth: les-at-zsgenetics.com 3, 52 -- X-Email-Count: 9 3, 52 -- X-Source-Cap: enNnYWRtMDE7enNnYWRtMDE7Z2F0b3IyMDEzLmhvc3RnYXRvci5jb20= ==============================End of - Headers==============================
I am considering purchasing a wall-mounted fume extractor for my microwave tissue processor which I will use for EM and paraffin work (to save space in my fume hood).
I have found some filters that have been rated for use with PFA and GA, but I am waiting to hear from the manufacturer regarding their use for OSO4, acetone, resins, and PO (doubt that info is available). I'd like to assume that the filters ("special carbon-treated") effectively filter out all of these, but I need to be as certain as possible. I will have undergraduates preparing samples and hope to use the microwave in my TEM and SEM courses, so I am very concerned about student safety.
Do any of you have any experience using these systems? Are they safe? Should I stop being so worried and just buy it? :)
Thank you! -Blanca ----------------------------------------- Blanca Carbajal Gonzalez, M.S. Director of Microscopy Science Center 50 College St Mount Holyoke College Clapp Laboratory 123 Office: 413-538-3118 Cell: 559-905-7138 bicarbaj-at-mtholyoke.edu MHC Microscopy
Why don't you run HVAC flex pipe from your wall-mounted fume extractor and plug it into the exhaust system? Failing that - modify one frame in the nearest window and exhaust filtered air outside. Decent carpenter or HVAC tech should be able to make such setup. Be safe :)
Valery Ray www.linkedin.com/in/valeryray ============================== PBS&T, MEO Engineering Company 290 Broadway, Suite 298 Methuen, MA 01844, USA Phone: +1-978-305-0479 - leave a message Mobie: +1-978-305-0479 - leave a message E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 1/17/2017 2:54 PM, bicarbaj-at-mtholyoke.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello everyone, } } I am considering purchasing a wall-mounted fume extractor for my } microwave tissue processor which I will use for EM and paraffin work } (to save space in my fume hood). } } I have found some filters that have been rated for use with PFA and } GA, but I am waiting to hear from the manufacturer regarding their use } for OSO4, acetone, resins, and PO (doubt that info is available). I'd } like to assume that the filters ("special carbon-treated") effectively } filter out all of these, but I need to be as certain as possible. I } will have undergraduates preparing samples and hope to use the } microwave in my TEM and SEM courses, so I am very concerned about } student safety. } } Do any of you have any experience using these systems? Are they safe? } Should I stop being so worried and just buy it? :) } } Thank you! } -Blanca } ----------------------------------------- } Blanca Carbajal Gonzalez, M.S. } Director of Microscopy } Science Center } 50 College St } Mount Holyoke College } Clapp Laboratory 123 } Office: 413-538-3118 } Cell: 559-905-7138 } bicarbaj-at-mtholyoke.edu } MHC Microscopy } } ==============================Original Headers============================== } 5, 37 -- From bicarbaj-at-mtholyoke.edu Tue Jan 17 13:53:10 2017 } 5, 37 -- Received: from mail-wm0-f41.google.com (mail-wm0-f41.google.com [74.125.82.41]) } 5, 37 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0HJr9bt008517 } 5, 37 -- for {microscopy-at-microscopy.com} ; Tue, 17 Jan 2017 13:53:10 -0600 } 5, 37 -- Received: by mail-wm0-f41.google.com with SMTP id c85so215510471wmi.1 } 5, 37 -- for {microscopy-at-microscopy.com} ; Tue, 17 Jan 2017 11:57:51 -0800 (PST) } 5, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 5, 37 -- d=mtholyoke-edu.20150623.gappssmtp.com; s=20150623; } 5, 37 -- h=mime-version:from:date:message-id:subject:to; } 5, 37 -- bh=i3zQfqAWrVLrQ5OUBetjF0orNpnlF8fG58FEU3N/gjQ=; } 5, 37 -- b=Ih3Zu1DKmSBwIjOXXfvaD8wUKZXMgQ/Skrs0fTiPrbLAfKUerCcDj+5TSipuaUEUqx } 5, 37 -- 6rWxgY+FIWULJTlWNwwnFQ+61mDDb576pKic9ky34Y7rush3Cy7xcAX7mDbO73czvICI } 5, 37 -- 8XJfquhBDMfUcWLVkUN0l26lz+Vcmy+GWNTN6gX0WsA8JtN+28afC98r7yqIERyOTXNi } 5, 37 -- 48JBpQ792PFU93GcGpaSayimqUw9d4waQrlsVczixHkDPDQY0TxTg/AUhROb/5MyQ0gN } 5, 37 -- +RyztwlnvLIs7ew+vcd8T/mcNp0BxICSby0xopSCHbczUtYJFjYhVGW/huiMC5rXggkx } 5, 37 -- LHWg== } 5, 37 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 5, 37 -- d=1e100.net; s=20161025; } 5, 37 -- h=x-gm-message-state:mime-version:from:date:message-id:subject:to; } 5, 37 -- bh=i3zQfqAWrVLrQ5OUBetjF0orNpnlF8fG58FEU3N/gjQ=; } 5, 37 -- b=U+if/Ewuf4u/L6Lf69/jPJQi+VP7M6dgS3AnWYc73In4vdpleVJMFKN8EQ9ibye14t } 5, 37 -- +eTfGWbyXpxUWJo26y36P+2e+bRaupqPbr3RiZAZ6MsW/s0PUJLzOsbPj8Tki8Vool5U } 5, 37 -- pQZZWFvS5u9qJv4+l7LPQr/YqktywVMpR3Gw08mTxu9CVZd5Zm9M8Rru53tsNbVuTMcD } 5, 37 -- kFrY9BbZ1tavtjN9JIX6jJZ04RvueA3HCT6Z6La9W71KPWuhv3LgcjtVLWXy33Ds/kZB } 5, 37 -- wERNwdwNtVDqNlufsR7LiW7QVxSTsOmzSTxRtjfE/Mft7uL1+v0V8x96rDGEkffvIFbQ } 5, 37 -- J+pg== } 5, 37 -- X-Gm-Message-State: AIkVDXJp+xHBYk0P2sZbfDj2eWFyJ5jIXAorGOhwBY8Ghqh+9Wi1U1OPT1bo/dn/X56HF7CQdZUcwARDFJBz4Z+O } 5, 37 -- X-Received: by 10.223.139.213 with SMTP id w21mr4712590wra.108.1484683070809; } 5, 37 -- Tue, 17 Jan 2017 11:57:50 -0800 (PST) } 5, 37 -- MIME-Version: 1.0 } 5, 37 -- Received: by 10.80.143.225 with HTTP; Tue, 17 Jan 2017 11:57:50 -0800 (PST) } 5, 37 -- From: Blanca Carbajal Gonzalez {bicarbaj-at-mtholyoke.edu} } 5, 37 -- Date: Tue, 17 Jan 2017 14:57:50 -0500 } 5, 37 -- Message-ID: {CAO94sumJEJEG0mw+2yoewmQSQRDodJE5CkOazdHvbhxzZB9vvg-at-mail.gmail.com} } 5, 37 -- Subject: EM: wall-mounted fume extractors } 5, 37 -- To: microscopy-at-microscopy.com } 5, 37 -- Content-Type: text/plain; charset=UTF-8 } ==============================End of - Headers============================== }
==============================Original Headers============================== 5, 35 -- From vray-at-partbeamsystech.com Tue Jan 17 15:06:27 2017 5, 35 -- Received: from nm7.bullet.mail.bf1.yahoo.com (nm7.bullet.mail.bf1.yahoo.com [98.139.212.166]) 5, 35 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0HL6RCd002096 5, 35 -- for {microscopy-at-microscopy.com} ; Tue, 17 Jan 2017 15:06:27 -0600 5, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1484687468; bh=l5cpaqW3TdyfzEAegATFvdK6g7ed2lq3S1IZPoJbhgc=; h=Subject:To:References:From:Cc:Date:In-Reply-To:From:Subject; b=IVa0gsZI7mBPw8PtaRUUdOtP1S0P1qEKvN2QjFLJRLXL3EJVOKlw0rstOfQ7T/SpQiqyoACLK5BUzGuHThzncXaeHJbbTyxX/YdqBZ4ZZRW3AAlD8B4fmptjqfViSGa8AV4hAiZup3nMkLdw0UBJ0df6zS1SE0tdVJ0ZH7So7ZX1qpiEfMo0GlFCArlX3yI7yrBdcSn518iLd6hWelE+ahU4oE7mvILSaOwygHC52F6O2KTFt5CuRHQEfDcThBvIXsrTKwRV+3ZEKTxKKMVQUOv9mKnJzE8b5OLgVwvjREkwU6IjHBCnsQoLkZyhEYw+LNnpn0cjKpqOrsnDAmnQNA== 5, 35 -- Received: from [98.139.215.143] by nm7.bullet.mail.bf1.yahoo.com with NNFMP; 17 Jan 2017 21:11:08 -0000 5, 35 -- Received: from [68.142.230.72] by tm14.bullet.mail.bf1.yahoo.com with NNFMP; 17 Jan 2017 21:11:08 -0000 5, 35 -- Received: from [127.0.0.1] by smtp229.mail.bf1.yahoo.com with NNFMP; 17 Jan 2017 21:11:08 -0000 5, 35 -- X-Yahoo-Newman-Id: 847699.48392.bm-at-smtp229.mail.bf1.yahoo.com 5, 35 -- X-Yahoo-Newman-Property: ymail-3 5, 35 -- X-YMail-OSG: YNOyXGEVM1lbZCj8QReoC7sPFpS.m7LNDCSzzp1zqIkfXyN 5, 35 -- PZfbaGJ.wrLJ6dxiP9vUeQQFqp2wp05lCT.SbNNWXA.IZClvNminro5.SgWv 5, 35 -- uQnZ39Lhi5781s8dBHIiBalFlcVWnhJ8296hAVCjczfjQtf_GMWq1oT4nsj5 5, 35 -- IdHlIWlXlArpkRG5krr8tik8ou9iC3V6ANZ6M62NbtZG4OOFqAMGglM8tjWN 5, 35 -- vEfogsc_WHzKUq.DHoik55BDAtLBpimLPHwlDybBQYEITpp4uo.mHr0vqW5w 5, 35 -- obkyV71bshLRhqQt.eX3Jedeh0FuB1Sg9OER4PnUyPpTy3cfGBibXCATHz8k 5, 35 -- a65Svg0.swVj8gfPBMzEUan4OtznTQJg0tt8qjqtRnLInYrdCllGayoaBwzz 5, 35 -- lhXbbMv1macilkjzFdeyEnyWA2bWlfzk6jjm.3vA1rY7wGauDOzpPGQJ0pI4 5, 35 -- m5cI1cHs4NjhTwlaCh9UFVF01spMVNK5_1hKKBtRnVyEroZHBr1wR358ywWF 5, 35 -- U1xbcdP6iKwQeAidkvS9ABwoqo6uVetgAoQcGyybMbDcrLXsxcBD1Ou2g.Ql 5, 35 -- R_ioR0MSA 5, 35 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 5, 35 -- Subject: Re: [Microscopy] EM: wall-mounted fume extractors 5, 35 -- To: bicarbaj-at-mtholyoke.edu 5, 35 -- References: {201701171954.v0HJsT6C009676-at-microscopy.com} 5, 35 -- From: Valery Ray {vray-at-partbeamsystech.com} 5, 35 -- Cc: microscopy-at-microscopy.com 5, 35 -- Message-ID: {7f10c025-790f-3b31-b7c4-ed6b6df19eff-at-partbeamsystech.com} 5, 35 -- Date: Tue, 17 Jan 2017 16:09:05 -0500 5, 35 -- User-Agent: Mozilla/5.0 (Windows NT 10.0; WOW64; rv:45.0) Gecko/20100101 5, 35 -- Thunderbird/45.6.0 5, 35 -- MIME-Version: 1.0 5, 35 -- In-Reply-To: {201701171954.v0HJsT6C009676-at-microscopy.com} 5, 35 -- Content-Type: text/plain; charset=windows-1252; format=flowed 5, 35 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mreedman-at-rms.org.uk as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: mreedman-at-rms.org.uk Name: Mel Reedman
Organization: Royal Microscopical Society
Title-Subject: [Filtered] MSM XX Abstract Submission Extended - Microscopy of Semi Conducting Materials
Message: Abstract deadline extended to Sunday 22 January and Registration now open! Microscopy of Semiconducting Materials XX (MSM XX) 9-13 April 2017 Lady Margaret Hall, University of Oxford
www.rms.org.uk/msm-xx
Scientific Organiser: Thomas Walther The conference will focus on the most recent advances in the study of the structural and electronic properties of semiconducting materials by the application of transmission and scanning electron microscopy. The latest developments in the use of other important micro-characterisation techniques including scanning probe microscopy, X-ray topography and diffraction will also be featured. Developments in materials science and technology covering the complete range of elemental and compound semiconductors will be described. Submit your abstract before midnight on Sunday 22 January 2017. We are accepting abstracts for both submitted talks and poster presentations at MSM XX on the following topics: - Analytical TEM - CL - Lattice Defects - Poly and Nano Crystals - Thin Films - Strained Layers and QWs - Nanowires - SPM & APFIM - SEM & FIB - Advanced Devices
Registration Registration for this meeting is now open. To register your place, please visit www.rms.org.uk/msm-xx. The registration fees are as follows:
Standard rate 575 RMS/IoP Member rate 475 Student rate 325 RMS/IoP Student member rate 280
To book en-suite accommodation for 75 a night directly with Lady Margaret Hall, please visit http://conference.lmh.ox.ac.uk/accommodation/. Use the code MSM2017, to show availability and take advantage of the preferential room rate. Find out more, submit an abstract and register at www.rms.org.uk/msm-xx
Login Host: 79.79.59.113 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
From ronasyre474-at-gmail.com Wed Jan 18 09:59:01 2017 Return-Path: {ronasyre474-at-gmail.com} Received: from gmail.com ([183.111.66.21]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v0IFwwPS018121 for {microscopylistserverarchive6-at-microscopy.com} ; Wed, 18 Jan 2017 09:59:00 -0600 Message-ID: {1C255798.5C7C2740-at-gmail.com}
X-from: erica-at-scripps.edu
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both erica-at-scripps.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Position available in San Diego, California
Message: Position available for an experienced staff scientist (or postdoc) to perform single particle cryoEM studies on proteins relevant for viral hemorrhagic fever pathogenesis and medical defense.
You will have primary responsibility for data collection and oversight of a dedicated 300 keV Titan Halo with a Falcon 2 direct detector and for training of incoming students to the lab. In particular, we are looking for someone with strong interest and expertise in instrumentation, good communication skills, and a collaborative spirit who could pursue their own projects and assist those of others.
Our lab studies the proteins of viruses like Ebola, Marburg and Lassa, using structural biology and cellular analysis to understand how the very few proteins encoded by these viruses achieve a tremendous array of functions through the virus life cycle. Although we are primarily a structural biology lab ourselves, we direct a five-continent collaboration of academic, industrial and government scientists and clinicians. Hence, there is the opportunity here to see how the structures fit into the cellular, organismal and population contexts and to use structural biology as a roadmap to devlop much-needed vaccines and therapeutics.
Applications should be sent via email to erica-at-scripps.edu and should include a c.v., list of publications, names of references and a letter of interest. If you have any questions, just email me!
Erica Ollmann Saphire, Ph.D. Professor, Immunology & Microbial Science Co-Director, Center of Excellence, The Global Virus Network Director, Viral Hemorrhagic Fever Immunotherapeutic Consortium
The Scripps Research Institute 10550 North Torrey Pines Road, IMM-21 La Jolla, CA 92037 Tel: (858)784-8602 erica-at-scripps.edu
Login Host: 137.131.212.56 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
Microscopy-at-Microscopy.com
============================================
==============================Original Headers============================== 17, 52 -- From microscopy.listserver-at-gmail.com Thu Jan 19 18:08:32 2017 17, 52 -- Received: from mail-it0-f42.google.com (mail-it0-f42.google.com [209.85.214.42]) 17, 52 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0K08VOK007751 17, 52 -- for {microscopy-at-microscopy.com} ; Thu, 19 Jan 2017 18:08:32 -0600 17, 52 -- Received: by mail-it0-f42.google.com with SMTP id c7so8978543itd.1 17, 52 -- for {microscopy-at-microscopy.com} ; Thu, 19 Jan 2017 16:13:21 -0800 (PST) 17, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 17, 52 -- d=gmail.com; s=20161025; 17, 52 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 17, 52 -- :mime-version:in-reply-to:content-transfer-encoding; 17, 52 -- bh=FbysXAEvLYxqxDbb3q6sOvH3jSrDTwAKwEQZjELvoA4=; 17, 52 -- b=ZxoDaA8AveTPUqzp9L3MICQPO50ylfzvEspYTrrx1V8HJhHP1D/4W9KurgTPGbX2rV 17, 52 -- xRZB4NX/PovUpfMnKaG0JHyfRwfUDXQCPjp9XN8bcY82eW0HiT5ciahdI7nahKtsiH/s 17, 52 -- 0m9kw/b5tqRw0stkPGwe6w2adgSnf9Q8uddjTR27Kf1U1nJy6miqJMeMoUiv1lU61Q5I 17, 52 -- P2Mjd3wFbJpjUKKeSocx95H1DUNOEgjM06Y2VITK+G3ewGKhnIMdiNDyXLlSUw7d5OVt 17, 52 -- rsf4eDJHOc1Y4+lOmm0J7Mcxm+Q1dIhdGQaaCxpFBAHI5pgrxjtL9uk2ZnCEjpsdrnHM 17, 52 -- JPuQ== 17, 52 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 17, 52 -- d=1e100.net; s=20161025; 17, 52 -- h=x-gm-message-state:from:subject:references:to:reply-to:message-id 17, 52 -- :date:user-agent:mime-version:in-reply-to:content-transfer-encoding; 17, 52 -- bh=FbysXAEvLYxqxDbb3q6sOvH3jSrDTwAKwEQZjELvoA4=; 17, 52 -- b=DaZf1ifUbLPLX+ehQflGEpFe1AqzSdBCtWuvjU38vK9KsirybONxnJoj3Xqs17wsyp 17, 52 -- S1jwTy1wZ6uO6ztJWuZ2eNRNw6MO0pwunrr5PjuCoACEhWXmXhoJpUwl+JLaD4Ak4yln 17, 52 -- 2WTtuXIcARl7PTO9Qyr4pZKmkoDr1XUL3See9W8uhYGIcBZKvbyEe82kWMhyys46H5ug 17, 52 -- lq9RTAHuQfLB+OEQoBuFvOH/AT4BQ7hatP1i9dN58pc6l75iyuziIWj0WeR10Rv/UuGv 17, 52 -- TB4OMAIqIGe+hu29Ysq3PUezv2EA2qnbkn8i633CwUSs+n6PtL3hGE1Zupsr5DhdSGcI 17, 52 -- LWCw== 17, 52 -- X-Gm-Message-State: AIkVDXKfGcgsafM8CM4936zaVEHA5sm+HnH58B5WBr6H54D7IbcokuBrBdjnZ5HTlTTO5g== 17, 52 -- X-Received: by 10.36.70.143 with SMTP id j137mr1237489itb.30.1484871201034; 17, 52 -- Thu, 19 Jan 2017 16:13:21 -0800 (PST) 17, 52 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:857e:1d84:d4a0:27fa]) 17, 52 -- by smtp.googlemail.com with ESMTPSA id j201sm463996ita.2.2017.01.19.16.13.20 17, 52 -- for {microscopy-at-microscopy.com} 17, 52 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 17, 52 -- Thu, 19 Jan 2017 16:13:20 -0800 (PST) 17, 52 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 17, 52 -- X-Google-Original-From: MicroscopyListserver-NoReply 17, 52 -- {microscopylistserver-noreply-at-microscopy.com} 17, 52 -- Subject: viaWWW CryoEM :Position available in San Diego, California 17, 52 -- References: {201701192020.v0JKKk56030157-at-microscopy.com} 17, 52 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 17, 52 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 17, 52 -- X-Forwarded-Message-Id: {201701192020.v0JKKk56030157-at-microscopy.com} 17, 52 -- Message-ID: {09eeabd6-25ac-b50c-d8b4-7f0edf09bfe5-at-microscopy.com} 17, 52 -- Date: Thu, 19 Jan 2017 18:13:19 -0600 17, 52 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:45.0) 17, 52 -- Gecko/20100101 Thunderbird/45.6.0 17, 52 -- MIME-Version: 1.0 17, 52 -- In-Reply-To: {201701192020.v0JKKk56030157-at-microscopy.com} 17, 52 -- Content-Type: text/plain; charset=windows-1252; format=flowed 17, 52 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jhyun-at-gatan.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: jhyun-at-gatan.com Name: John Hyun
Organization: Gatan
Title-Subject: [Filtered] TEM Application Scientist Opening
Message: Location: Pleasanton, CA (remote locations considered)
Position Summary: This position is responsible for supporting the sales and marketing efforts of Gatans highly successful Analytical TEM product line (GIF and EELS systems). The position is based at Gatans Pleasanton, CA headquarters but will support analytical products worldwide.
Job Responsibilities: Provide pre-sales applications support with presentations and demonstrations of Gatan products on a range of TEM models Provide post-sales customer support and on-site customer training Provide on-site advanced customer training and telephone/email support Provide general applications support, telephone/email support, and limited hardware and software troubleshooting Propose design enhancements and improvements to Gatan hardware and software Work with Gatan R&D in the development and testing of new products and applications Represent Gatan at scientific conferences
Education/Experience: Advanced degree in science or engineering (or equivalent experience) is required Strong background in transmission electron microscopy, specifically in relation to the acquisition, application, interpretation, and discussion of analytical TEM techniques including EELS, EDS, EFTEM, and STEM techniques Hands-on, post-graduate level experience in advanced analytical TEM applications with a proven publication record Qualifications: Ability to interface effectively with customers at all experience levels while projecting a strong client service attitude Excellent verbal, written, and interpersonal communication skills in English are essential Ability to work on complex learning and development problems as well as teach highly technical information is essential Significant domestic and international travel required- 50% or higher at times.
To Apply: Interested candidates should submit a resume to hrus-at-gatan.com. Gatan is an equal opportunity employer. All qualified applicants will receive consideration for employment without regard to race, religion, color, national origin, sex, sexual orientation, gender identity, age, status as a protected veteran, among other things, or status as a qualified individual with disability.
Login Host: 50.58.128.11 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both heckman-at-bgsu.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: heckman-at-bgsu.edu Name: Carol Heckman
Organization: Bowling Green State University
Title-Subject: [Filtered] Gatan ChromaCL
Message: We bought a GATAN cathodoluminescence detector some years ago, and had it installed on our Hitachi 2700S SEM. It breaks vacuum sometimes when it is entered into the column. We need cathodoluminescence mode for several projects.
Does anybody have the same problem? If so, what did you do and did you solve it?
Carol Heckman
Login Host: 129.1.85.73 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
Since the problem is intermittent and occurs during insertion, chances are you have either crack in vacuum bellows, or warn-out O-ring somewhere in feedthrough. You would probably need to call in either OEM or third-party service, or find someone local who can bring in a leak detector and troubleshoot this on site.
Best Wishes :)
Valery Ray www.linkedin.com/in/valeryray ============================== PBS&T, MEO Engineering Company 290 Broadway, Suite 298 Methuen, MA 01844, USA Phone: +1-978-305-0479 - leave a message Mobie: +1-978-305-0479 - leave a message E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 1/21/2017 3:53 PM, microscopy.listserver-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both heckman-at-bgsu.edu as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: heckman-at-bgsu.edu } Name: Carol Heckman } } Organization: Bowling Green State University } } Title-Subject: [Filtered] Gatan ChromaCL } } Message: We bought a GATAN cathodoluminescence detector some years ago, and had it installed on our } Hitachi 2700S SEM. It breaks vacuum sometimes when it is entered into the column. We need } cathodoluminescence mode for several projects. } } Does anybody have the same problem? If so, what did you do and did you solve it? } } } Carol Heckman } } } Login Host: 129.1.85.73 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } }
==============================Original Headers============================== 5, 35 -- From vray-at-partbeamsystech.com Sat Jan 21 16:57:54 2017 5, 35 -- Received: from nm17-vm1.bullet.mail.bf1.yahoo.com (nm17-vm1.bullet.mail.bf1.yahoo.com [98.139.213.55]) 5, 35 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0LMvr5W004903 5, 35 -- for {microscopy-at-microscopy.com} ; Sat, 21 Jan 2017 16:57:54 -0600 5, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1485039770; bh=190jyd0D/6uDYtpxJWYjPSZROobRZ5hN53vjca3ckd4=; h=Subject:To:References:Cc:From:Date:In-Reply-To:From:Subject; b=J4AAeJARsZgWxhgsCMqbnHTUFZpS27ftaQIoqGwU5n0Qg4XljClwS68NqMJZ40b9i83m3bKWCCEUgMSIPL4otei91LEXPTUCmJeh0t2ZipI7jUe3gTcsO/tdBT4VhSP/za6D8diPtWoq33nmmSxX/sr2g5WSt2F9gDtdtUwuPaXq/2mNVNDVDVPTdCpdp+eRoV4bztzRS2dXYvbqnhk/9R0m/EXupXjWW3zI+zyiNCIPVSBPfLt1yPuFQslbEcsipc3+1UQCILwqU10oGSFm0gBlQ6J+TCtVolOpXY2nq05GEsRjneTeCC5aGCTxiNTeVaHY1aRXAP64VNd/hl5liQ== 5, 35 -- Received: from [98.139.215.142] by nm17.bullet.mail.bf1.yahoo.com with NNFMP; 21 Jan 2017 23:02:50 -0000 5, 35 -- Received: from [98.139.211.203] by tm13.bullet.mail.bf1.yahoo.com with NNFMP; 21 Jan 2017 23:02:50 -0000 5, 35 -- Received: from [127.0.0.1] by smtp212.mail.bf1.yahoo.com with NNFMP; 21 Jan 2017 23:02:50 -0000 5, 35 -- X-Yahoo-Newman-Id: 271930.99062.bm-at-smtp212.mail.bf1.yahoo.com 5, 35 -- X-Yahoo-Newman-Property: ymail-3 5, 35 -- X-YMail-OSG: gjHUqHkVM1k8KApY7QGPOWJUzZ.RBYwDDsu73Xm.0hcMFgY 5, 35 -- XheruY2ddVZ8sRXDt3qe6VukrUSQRnGWM3579WAy9Me0uNKe5OVl_1OggmgW 5, 35 -- yb4I9UoVJBeuZT4se7sFoukN08xHeBkhL8XXMb7d.tMf2Heqd.KqV9ULrRjI 5, 35 -- mBWGoEfICnw6Vavtp_U4hYOrRW0o6spIGHOVccdytFmi73PAnVGcst1nes.v 5, 35 -- o9wdXHQspbFq_.36oSbNkKMIJpG6uMpC4hBCFyUF6pyJXXt83EODLk8DFAUg 5, 35 -- jsNUVNdq8lGgQxIH.jykaLZIaQm7pwjCfU7LM33RNVrACnf6jXadDroCaHq2 5, 35 -- 9waWCa5cPn2oZdFFyma.Y7tXEUoOnX7tLsxbx5tNAQSktMikrCFstVayoYy1 5, 35 -- 1TQ.1w30Tv_kJVAHclW2Bu1WkqkvHg6LVwXzPfu86ndGEwol8u9joIM.rkha 5, 35 -- BPnDm1eMSwEBhcv5u4ZT5l5FrOQdocAnBPr5O1fV5EYgsIFYCx9T9zkSuO3n 5, 35 -- DbruOdBrD9bvzs4a85man.08wC0a39.Jt1z0qUzioBgOq7DCdjmd3usZRr3i 5, 35 -- M45HlTJc4 5, 35 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 5, 35 -- Subject: Re: [Microscopy] viaWWW: Gatan ChromaCL Help Needed 5, 35 -- To: heckman-at-bgsu.edu 5, 35 -- References: {201701212053.v0LKrG0Q012609-at-microscopy.com} 5, 35 -- Cc: microscopy-at-microscopy.com 5, 35 -- From: Valery Ray {vray-at-partbeamsystech.com} 5, 35 -- Message-ID: {69cb53e9-760a-5666-5610-062fd2d21155-at-partbeamsystech.com} 5, 35 -- Date: Sat, 21 Jan 2017 18:00:38 -0500 5, 35 -- User-Agent: Mozilla/5.0 (Windows NT 10.0; WOW64; rv:45.0) Gecko/20100101 5, 35 -- Thunderbird/45.6.0 5, 35 -- MIME-Version: 1.0 5, 35 -- In-Reply-To: {201701212053.v0LKrG0Q012609-at-microscopy.com} 5, 35 -- Content-Type: text/plain; charset=windows-1252; format=flowed 5, 35 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
From sawijama5-at-gmail.com Sat Jan 21 19:21:49 2017 Return-Path: {sawijama5-at-gmail.com} Received: from gmail.com ([210.92.18.86]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v0M1LkoN032594 for {microscopylistserverarchive6-at-microscopy.com} ; Sat, 21 Jan 2017 19:21:48 -0600 Message-ID: {18CCB40A.A1DE92E8-at-gmail.com}
All, For those interested in the microanalysis side of things, I have available to donate a Physical Electronics Quantum 2000 Scanning ESCA Microprobe. The Quantum is the predecessor to the PHI Quantera model.
This X-ray Photoelectron Spectroscopy/Electron Spectroscopy for Chemical Analysis system has been sitting powered down but connected to clean nitrogen for a little over a year, after developing a problem in a small power supply. That power supply has been priced at about $300. Prior to that, it had a noise issue, so I believe there is a problem with the detector or in the detector electronics that would need to be addressed. The unit was purchased in 1999 for approximately $600,000, and used for several years with very clean samples. In 2006 it was upgraded to a Windows XP data system.
My company is relocating R&D facilities from Duncan, SC (the current instrument location) and no longer has pressing need for the technique. Rather than relocate the instrument, we would like to donate it to a university or other non-profit. Recipient would be responsible for transportation and related costs. As part of an effort to give back to our local community, preference would be given to schools in closer proximity to our new headquarters in Charlotte, NC, but all interested parties are encouraged to inquire by emailing me at james.passmore+xps-at-sealedair.com.
Regards, Jim
-- Jim Passmore Principal Scientist, R&D
Sealed Air Corporation P: 864.433.2927 100 Rogers Bridge Rd., Bldg A Duncan, SC 29334
==============================Original Headers============================== 6, 34 -- From james.passmore-at-sealedair.com Mon Jan 23 08:10:43 2017 6, 34 -- Received: from mail-wm0-f48.google.com (mail-wm0-f48.google.com [74.125.82.48]) 6, 34 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0NEAg9I016510 6, 34 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Jan 2017 08:10:42 -0600 6, 34 -- Received: by mail-wm0-f48.google.com with SMTP id c85so134635118wmi.1 6, 34 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Jan 2017 06:15:45 -0800 (PST) 6, 34 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 34 -- d=sealedair.com; s=google; 6, 34 -- h=mime-version:from:date:message-id:subject:to; 6, 34 -- bh=6VLh4FtX3pQY8h/IfK0aO6Hi3FYP7s+9gDIC1IuQkFc=; 6, 34 -- b=bV1xRioQ4lu3ejK9LWwtia2P1OKG1kdfmusDLvrL3cSkGqheV7RcVh9XoADmTFSWPK 6, 34 -- K1LPOhu40KfscbS+LGIwFIxBYDT0HGFxSis3onRahs9zxME2Yu6+gYejsV/VtCLISrE5 6, 34 -- bDNWG2JR7LNgKLkiSDbKT/OGxF7Q9IOCO/OYM= 6, 34 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 34 -- d=1e100.net; s=20161025; 6, 34 -- h=x-gm-message-state:mime-version:from:date:message-id:subject:to; 6, 34 -- bh=6VLh4FtX3pQY8h/IfK0aO6Hi3FYP7s+9gDIC1IuQkFc=; 6, 34 -- b=Q2LuygXFF8CYahuQdGfUbxDR61fcnXr1ti0+MNynWAfuR4jorZBOWOu4gVej/OTqPp 6, 34 -- PLd5JYfTz+SpvaJOKyAu/YpYS+R4M9U0zoAN5cPw666iNoiEBrB2Mbb5jUse+5jXrqoZ 6, 34 -- 5LrvSmWY9vJ/bgKxve2jgYNbai2XXGlULLmyWQxqAt/D+Hnyq6KIHcCnNEhMdy3nF/i8 6, 34 -- Sgq5v7FmoUXTRhWOSMk0rAEJalt6TwtpsbAZw//xvUhb6xvtcVl7rjTwl+YbWOMIx8V1 6, 34 -- PCVv4SyaO69pcOsCoLQhIFBV1uFlRelEcUBsXvtEqeLc82hbdE5yQ4c1Nr2LcfpEgkSD 6, 34 -- xBQA== 6, 34 -- X-Gm-Message-State: AIkVDXLcejNUpUufk0JEvmMi+q+jA59jQqOw584Eyl4JRBTUsVszJUkpKmb6slBb0RBz/SGgBFOyBsfhpXoRv7zG 6, 34 -- X-Received: by 10.223.136.155 with SMTP id f27mr23112431wrf.98.1485180944288; 6, 34 -- Mon, 23 Jan 2017 06:15:44 -0800 (PST) 6, 34 -- MIME-Version: 1.0 6, 34 -- Received: by 10.194.239.234 with HTTP; Mon, 23 Jan 2017 06:15:13 -0800 (PST) 6, 34 -- From: Jim Passmore {james.passmore-at-sealedair.com} 6, 34 -- Date: Mon, 23 Jan 2017 09:15:13 -0500 6, 34 -- Message-ID: {CAGV-iYZ78u+nQiejE1a46KNBRXz=8cW7pMToEGDphbW-L7V0-g-at-mail.gmail.com} 6, 34 -- Subject: XPS/ESCA system for donation 6, 34 -- To: microscopy {Microscopy-at-microscopy.com} 6, 34 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both cni-at-udel.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: cni-at-udel.edu Name: Chaoying Ni
Organization: University of Delaware
Title-Subject: [Filtered] Research Associate I (28N) - Electron Microscopy
Message: Research Associate I - (Grade 28N) Materials Science and Engineering
Deadline: January 26, 2017
CONTEXT OF THE JOB:
Under limited supervision, performs a variety of complex technical laboratory duties including the design and construction of test equipment. Supervises and trains personnel. The principal emphasis is on working independently and exercising personal initiative in conducting experiments using complex electron and light microscopes, scanning probe microscopes and associated sample preparation equipment and experiment devices.
QUALIFICATIONS:
Requires a minimum of a Bachelor's degree and one year of experience in laboratory work in a relevant field as well as experience with the necessary physical, electronic and chemical systems. Supervisory experience preferred. Related progressive experience beyond a high school diploma or GED may be substituted for required education or additional related education may be substituted for required experience. Requires knowledge of laboratory techniques, procedures and instrumentation.
Requires abilities to read and interpret complex operations manuals, drawings and design, interpret problems and design equipment for the particular tasks, assume responsibility and take initiative to perform tasks, work independently, train and instruct others, and communicate effectively and interact well with people of all ages and diverse backgrounds.
OCCUPATIONAL EXPOSURES:
May be required to use personal protective equipment to prevent exposure to hazardous materials.
More details: https://udjobs.nss.udel.edu:4450/psc/RESUME/EMPLOYEE/HRMS/s/WEBLIB_UDEL.ISCRIPT1.FieldFormula.IScript_Careers?Page=HRS_CE_HM_PRE&Action=A&SiteId=888&
or
http://www1.udel.edu/udjobs/
Login Host: 128.4.80.105 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
Microscopy-at-Microscopy.com
============================================
==============================Original Headers============================== 24, 53 -- From microscopy.listserver-at-gmail.com Mon Jan 23 18:14:01 2017 24, 53 -- Received: from mail-io0-f171.google.com (mail-io0-f171.google.com [209.85.223.171]) 24, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0O0E1Pl004300 24, 53 -- for {microscopy-at-microscopy.com} ; Mon, 23 Jan 2017 18:14:01 -0600 24, 53 -- Received: by mail-io0-f171.google.com with SMTP id v96so60486320ioi.0 24, 53 -- for {microscopy-at-microscopy.com} ; Mon, 23 Jan 2017 16:19:06 -0800 (PST) 24, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 24, 53 -- d=gmail.com; s=20161025; 24, 53 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 24, 53 -- :mime-version:in-reply-to:content-transfer-encoding; 24, 53 -- bh=CzCmc0vsbop3kHNGOaGbLQZJuoCHweD6EXJPFY61xmg=; 24, 53 -- b=GHgB+V7ErnJPx337A/ayYcrhEvkkxTJcCz+iXDY0ytqsIMx1p4cS0t1FqJq6Cixmq5 24, 53 -- JT886Temr1pzXNTb2vCOtXOh19s4L/L9t6Wag81pQqDqFVUXTcTYOzMPrbhuGdIfcht6 24, 53 -- YCP6KrWeRZAwu3QxzcLUFRfuLEaGNUnLxBErZtnd0b9rrWLlHMfch8eutuj6h2nDnBNS 24, 53 -- 1linZeRl65aEgMQVbxQvql2NxQMH8AoKYAnrLD5PaB1EgcSEFKwzqRjMvZi0wTkn4+bh 24, 53 -- nWlJ230wHkj3fCw2W53N9EbYYP0pqXjeZmebRmamNjhUBz9wn8MJn39+wdZxQRSTIZ6D 24, 53 -- kYEA== 24, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 24, 53 -- d=1e100.net; s=20161025; 24, 53 -- h=x-gm-message-state:from:subject:references:to:reply-to:message-id 24, 53 -- :date:user-agent:mime-version:in-reply-to:content-transfer-encoding; 24, 53 -- bh=CzCmc0vsbop3kHNGOaGbLQZJuoCHweD6EXJPFY61xmg=; 24, 53 -- b=TYGslxLzqixT4MX99gzhbC7UILgyY5OvWSq/y9p0EF0L7mXB7rMraGf8guqBqrddzh 24, 53 -- d1hmErbSsczwXn6a+7eRep0m7ExplXF8K13aN9r4bj8F8bZckTXrA02HoxWDCb1e+4Kz 24, 53 -- RlGrqJoL5+46uXzyivL6I1YhwtYyQAswVfSYlAlsucn+1mqCwBbBxIxFj4pVtur0kpoq 24, 53 -- CCDgFbff6wkWxaTzzP2EoeSYBtc58k+AILZz6K/5MTE+whG3JjDfJnxp4A2PY8MBcFHE 24, 53 -- ye5929DVhxRoLW49etAZMJzVgps6pGk01Csf12p5+aXbDWNLdNyUXatQ7105lfWbM4uo 24, 53 -- 8nkg== 24, 53 -- X-Gm-Message-State: AIkVDXLQzd5FfwasOCLi4wHDo6IzsCUp9NUPSLUQ1WcvW+t0RfgTDC8JCG1LyOcj0gqWDg== 24, 53 -- X-Received: by 10.107.59.69 with SMTP id i66mr3085383ioa.10.1485217145431; 24, 53 -- Mon, 23 Jan 2017 16:19:05 -0800 (PST) 24, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:6493:1e90:3d3a:7abd]) 24, 53 -- by smtp.googlemail.com with ESMTPSA id q89sm5590992ioi.42.2017.01.23.16.19.04 24, 53 -- for {microscopy-at-microscopy.com} 24, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 24, 53 -- Mon, 23 Jan 2017 16:19:04 -0800 (PST) 24, 53 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 24, 53 -- X-Google-Original-From: MicroscopyListserver-NoReply 24, 53 -- {microscopylistserver-noreply-at-microscopy.com} 24, 53 -- Subject: viaWWW: Position Open : Research Associate I (28N) - Electron 24, 53 -- Microscopy 24, 53 -- References: {201701232133.v0NLXnDQ028268-at-microscopy.com} 24, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 24, 53 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 24, 53 -- X-Forwarded-Message-Id: {201701232133.v0NLXnDQ028268-at-microscopy.com} 24, 53 -- Message-ID: {23fdf9bc-5b36-0f9a-e963-01c0cb2651cd-at-microscopy.com} 24, 53 -- Date: Mon, 23 Jan 2017 18:19:03 -0600 24, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:45.0) 24, 53 -- Gecko/20100101 Thunderbird/45.6.0 24, 53 -- MIME-Version: 1.0 24, 53 -- In-Reply-To: {201701232133.v0NLXnDQ028268-at-microscopy.com} 24, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed 24, 53 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mikeh-at-ncimicro.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: mikeh-at-ncimicro.com Name: Mike Hehr
Organization: NCI
Title-Subject: [Filtered] Nano Technology Sales Position
Message: We are currently seeking an individual with EM Sample Prep experience for a new technical sales position. This includes, Ion Milling, Ultra Microtomy, CLEM and High Pressure Freezing. This role will be to support customers in the vast Midwest. If interested, please submit you resume to mikeh-at-ncimicro.com
Login Host: 23.25.147.133 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
Those of you actively working with FIB in either device or analytical applications could find the forwarded below message of interest:
--- On Tue, 1/24/17, Hugo Bender (imec) {Hugo.Bender-at-imec.be} wrote:
} From: Hugo Bender (imec) {Hugo.Bender-at-imec.be} } Subject: new link to EFUG website : http://efug.imec.be/ } To: "Hugo Bender (imec)" {Hugo.Bender-at-imec.be} } Date: Tuesday, January 24, 2017, 1:18 AM } } Dear EFUG’ers, } FIB’ers, } } } Please note that the link to the } EFUG website is changed to : http://efug.imec.be/ } } The old link will soon not be } operational anymore. So update your bookmarks } now! } } } There will be again 2 FIB meetings } in Europe in 2017: } } 1st EUFN } workshop } (Former DACH } Workshop) : general FIB and FIB } applications } 4-5 July 2017, Graz, Austria. } } Abstract : deadline 30 April 2017 } http://www.eu-f-n.org/ } } } 21st } EFUG meeting } during ESREF : } semiconductor and device applications of } FIB } Week 25-29 September 2017, } Bordeaux France } http://efug.imec.be/ } } } Please forward to your FIB } colleagues ! } } } Best regards } } Hugo } } } Hugo } Bender } Principal Member } Technical Staff } T +32 16 28 } 1304 } hugo.bender-at-imec.be } I } www.imec.be } Kapeldreef 75 } } I 3001 } Leuven } I } Belgium } } imec e-mail } disclaimer } } } } } }
==============================Original Headers============================== 5, 32 -- From vray-at-partbeamsystech.com Tue Jan 24 15:01:19 2017 5, 32 -- Received: from nm36-vm9.bullet.mail.gq1.yahoo.com (nm36-vm9.bullet.mail.gq1.yahoo.com [98.136.216.170]) 5, 32 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0OL1Jv9003076 5, 32 -- for {microscopy-at-microscopy.com} ; Tue, 24 Jan 2017 15:01:19 -0600 5, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1485291986; bh=dFUfiK1R0Nwm1ASBGNFmLH70A5tNNwLm2p8kH/0EcFQ=; h=Date:From:Reply-To:To:Subject:References:From:Subject; b=Im9wVUndp0XW1PhTD0+X7vFPshTWlSH74EbOHWLANUuSM6Wg/kFVbfIVhVEFw1KHZRRjBZ2s2N6CihsqyTOlm5+Azw6rohBJMPGOqBUu0zAVLc8+rBca9j+HlGOxTrOZwYAaomrij9Kn/KsfG734i2Xr+5IBtW6gmS+O3Mm6bbC4I/omkN6E8qgRtH9tOsPttvA2FegxppTf4hIRvhinpj0y5YrQxRjgJPdhVZomNn6JzZcGIwt53Y8Ed+tfdGaZXG/bK2AJHbvbqqqkdJ6FoXw0HwsAaOuypzIt6bdXhgi2obHSaIJyjXzBo9zwYh1xcGQG1BoODncXRNb//t9Mxg== 5, 32 -- Received: from [127.0.0.1] by nm36.bullet.mail.gq1.yahoo.com with NNFMP; 24 Jan 2017 21:06:26 -0000 5, 32 -- Received: from [216.39.60.183] by nm36.bullet.mail.gq1.yahoo.com with NNFMP; 24 Jan 2017 21:03:13 -0000 5, 32 -- Received: from [98.139.170.180] by tm19.bullet.mail.gq1.yahoo.com with NNFMP; 24 Jan 2017 21:03:13 -0000 5, 32 -- Received: from [98.139.215.229] by tm23.bullet.mail.bf1.yahoo.com with NNFMP; 24 Jan 2017 21:03:13 -0000 5, 32 -- Received: from [127.0.0.1] by omp1069.mail.bf1.yahoo.com with NNFMP; 24 Jan 2017 21:03:13 -0000 5, 32 -- X-Yahoo-Newman-Property: ymail-4 5, 32 -- X-Yahoo-Newman-Id: 420308.34066.bm-at-omp1069.mail.bf1.yahoo.com 5, 32 -- X-YMail-OSG: wLtB7pUVM1kVpu5oGhSOQ05wi1KTgP762ggHuisxFiTI.sSES33ux5T8Cf3yeP2 5, 32 -- OP67hpheJqYAXzxYIvNEOXW27zfVt.RbuM8C7Ctxuo3dZbNXOcfQkcmtjfCjRmrxq6ASu3tjR8mZ 5, 32 -- RUcqoW4XWViD6w.TNsNcUuyqhKkFkAe7D41EVMaI9rxK1bk861zvkJ2IiK6CKe4cQCavxS7WYUQC 5, 32 -- 8ceR7jvD37v7n6Osc6Y4sAJrduKH1e.IgUiSRwHgkkIzdoAFeIBpFCPaNCM8kCrHPxEHQinX1o.Q 5, 32 -- rDJYROriwwLTS5b_ib0uxM0oSrPb0vWPRXe1z9DXh01_dwLTxrQSuUbgMLD1qcX1N.3VIR86E6fZ 5, 32 -- 4yKhmse1lCjcaDc8vTTO6djp6fOceRFiJDeeSXLSb.ELwpdBbM7bX7QU08JXk_WJ6ipauk886ej6 5, 32 -- _10.DQqwf.llmH2uMEU91ps9bGpUgPHVFUjZMBpKdSXuSF1IO2g2gUcephHmT3KNuMDuK3sDU9Uw 5, 32 -- 2oWPAJNWRigRUJubS2RtRtGJ5m2Tlh80YcFLybA7qFHOkte8FtF9Hv0PPsUsMIOlIrA-- 5, 32 -- Received: from jws400105.mail.bf2.yahoo.com by sendmailws106.mail.bf1.yahoo.com; Tue, 24 Jan 2017 21:03:13 +0000; 1485291793.041 5, 32 -- Date: Tue, 24 Jan 2017 21:03:12 +0000 (UTC) 5, 32 -- From: {vray-at-partbeamsystech.com} 5, 32 -- Reply-To: {vray-at-partbeamsystech.com} 5, 32 -- To: {microscopy-at-microscopy.com} 5, 32 -- Message-ID: {1986517122.3093933.1485291792844-at-mail.yahoo.com} 5, 32 -- Subject: Fw: New link to EFUG website and EFUG/EUFN meeting announcements 5, 32 -- MIME-Version: 1.0 5, 32 -- Content-Type: text/plain; charset=UTF-8 5, 32 -- References: {1986517122.3093933.1485291792844.ref-at-mail.yahoo.com} 5, 32 -- Content-Transfer-Encoding: 8bit 5, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v0OL1Jv9003076 ==============================End of - Headers==============================
From michcons458-at-gmail.com Tue Jan 24 16:35:33 2017 Return-Path: {michcons458-at-gmail.com} Received: from gmail.com ([210.92.18.86]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v0OMZUvo027260 for {microscopylistserverarchive6-at-microscopy.com} ; Tue, 24 Jan 2017 16:35:32 -0600 Message-ID: {4149520D.C242A3C7-at-gmail.com}
I am a relatively inexperienced TEM tech and am having problems with my PTA. Each time I make fresh PTA, I end up with an image full of stain blobs. Obviously this is useless. I have tried purchasing fresh PTA, but that didn't make any difference. I have also used BSA, which helps some, but not a lot. Help! Any ideas are welcome.
Jami
Jami Carroll Lab Tech II - Virology Breathitt Veterinary Center Hopkinsville, KY 270-886-3959 jcarroll-at-murraystate.edu
==============================Original Headers============================== 3, 41 -- From jcarroll-at-murraystate.edu Wed Jan 25 14:29:39 2017 3, 41 -- Received: from isenguard1.murraystate.edu (isenguard1.murraystate.edu [216.249.159.66]) 3, 41 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0PKTbsW003097 3, 41 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Jan 2017 14:29:38 -0600 3, 41 -- Received: (qmail 9596 invoked by uid 514); 25 Jan 2017 14:36:00 -0600 3, 41 -- Received: from mail-io0-f200.google.com (209.85.223.200) 3, 41 -- by isenguard1.murraystate.edu with AES128-SHA encrypted SMTP; 25 Jan 2017 14:36:00 -0600 3, 41 -- Received: by mail-io0-f200.google.com with SMTP id j13so22886139iod.6 3, 41 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Jan 2017 12:34:48 -0800 (PST) 3, 41 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 41 -- d=murraystate.edu; s=google; 3, 41 -- h=from:mime-version:thread-index:date:message-id:subject:to; 3, 41 -- bh=TbOf5vsspNPFILyzcNL/dvwC+HvU+9H0+31YCGmgl/E=; 3, 41 -- b=WZ6VhdSIqrdc5oOkZa7X1Rmj+5334BWEDO33FudPIC3aJovIJXOWOrdWfyhmfHMaUJ 3, 41 -- 3lyo64W+gSTWA9EkIT/48KPnSDGAbk8beOzVQcqGvxuV5ygp/Q1N6BEuM5EHhIrlpQSb 3, 41 -- Ls9EbdTtw7y4Kjg32hUlIzYyg9SCW9KYjMLsc= 3, 41 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 41 -- d=1e100.net; s=20161025; 3, 41 -- h=x-gm-message-state:from:mime-version:thread-index:date:message-id 3, 41 -- :subject:to; 3, 41 -- bh=TbOf5vsspNPFILyzcNL/dvwC+HvU+9H0+31YCGmgl/E=; 3, 41 -- b=Jn648cp78H0CzTgjnBLa8maTkzAHTTnAjlUfEZ/A0n8eozRDZ1HZRy2ReC6H6SPo56 3, 41 -- bprdzy1+8O1V1+5s6Fi3E13J0Ad4Hrgfm5OviMFW3WOqsc3bBvxpWgTf4DGwsVfDnha+ 3, 41 -- QgpdEVd3DTrzyhlnkoYN0PjkunxisCYDWKWSyMEg2Wx1W613he2omkJzb2+PzIAJPdCJ 3, 41 -- +Xcmtjb26k25Y2bYIF/KvVqah+R1NAqLabeL5mHeoUhTzzRz96TTTutM1UWcU298LDCn 3, 41 -- 6qBeB5JCKN3XtvNe7W+qQq0b3+5GWoPEK0MUR610ZX8KhzPMlowdnpl+zMM+058oyUhf 3, 41 -- oVQQ== 3, 41 -- X-Gm-Message-State: AIkVDXJEKzPrp/T8tSiVuZJre5xfryNuP0448Sa+UFXWZ4MC9fvb9dc6VeOi4qI+6KSXlDOH48VQv+wBIH8OkDdlGeluC39/fcXLBOUu0V7jOSAG6EmBQfFpOu2Ju50ZUGfZAWmRG2h7rjRFAz3WXU7rbu6r5YzvzAI= 3, 41 -- X-Received: by 10.107.10.24 with SMTP id u24mr34419759ioi.94.1485376487879; 3, 41 -- Wed, 25 Jan 2017 12:34:47 -0800 (PST) 3, 41 -- X-Received: by 10.107.10.24 with SMTP id u24mr34419752ioi.94.1485376487758; 3, 41 -- Wed, 25 Jan 2017 12:34:47 -0800 (PST) 3, 41 -- From: Jami Carroll {jcarroll-at-murraystate.edu} 3, 41 -- MIME-Version: 1.0 3, 41 -- X-Mailer: Microsoft Outlook 15.0 3, 41 -- Thread-Index: AdJ3SlzXhP0ZYrc6S9+CcE8EBSgGPw== 3, 41 -- Date: Wed, 25 Jan 2017 14:34:46 -0600 3, 41 -- Message-ID: {316b19cf1154b769f967a2c0a91d4a27-at-mail.gmail.com} 3, 41 -- Subject: TEM negative staining issues 3, 41 -- To: Microscopy-at-microscopy.com 3, 41 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
I do hope that you have consulted Google with: "PTA negative staining" to try to find some troubleshooting advice from experts. What I am about to offer is all true and necessary for me. Thus, necessary for everyone else - if I don't think about your problem.
I can state with absolutely strictly personal experience, that one - Me! - throws time away all the time if the water one - Me, again - starts with has not been deionized, glass distilled, filtered with the 22um ['micro' or 'Meuw' meter] 57mm Millipore filter, and, if to be used in MolBio, laced with RNAse Inhibitor [https://en.wikipedia.org/wiki/Ribonuclease_inhibitor ]. Once this 'Monkish' procedure has been completed with both song and dance, I can be assured of a good experiment.
If the 'blobs' are larger than .22um, then I recommend that you run your PTA thru a 0.22um 12mm Millipore filter using a small Swinney holder with a 1ml disposable syringe. You could also try a dose of high frequency sonic jewelry cleaner borrowed from your Mom or your 'gal.'
I have given you both mystic and sound recommendations to attack your problem. These are things I have tried in the past, but, I admit, nowhere near Kentucky. [In the old days, histologists who Wintered in Maine and Summered in Florida, had two types of paraffin: one for Maine; and a second for Florida - each with a 'Climatized' paraffin recipe!
I do humarize when the subject permits, so, please understand that I am old (77), sometime called "Grumpy39," and cannot restrain myself any more.
Cheers, and good luck,
Fred Monson
P.S. If you come to a moment where you simply have to change brains, sit down and answer this simple question: "How many digital cameras do you have with you right now? After you answer, please take a few minutes to determine why some old guy would ask such a silly question to take my mind of my problem with PTA. There you go. My answer is that 98% of educated people give the wrong answer. You should know that I only gave ONE multiple answer test in the 12 years that I instructed in biology in development and electron microscopy. In histology, I felt obligated to remind students that all they would learn in histology would be about dead stuff. Cheers, again.
P.S. With that cheerful business done, please remember that life/death of virions is still under misunderstanding. Be careful - always. P.S. In the creek, doped with E. Coli, for each of them there are 100 phage. P.S. Oh! Yes! If you have a need of concentrating the sample, consider a Beckman Airfuge with a TEM head.
Frederick C. Monson, PhD Technical Director Center for Microanalysis and Imaging, Research and Training (CMIRT) West Chester University of PA Geology-Astronomy 750 South Church St. West Chester, PA, 19383 fmonson-at-wcupa.edu 610-738-0437(Work) Fc.monson-at-gmail.com for more help if you need it: i.e., refereces, and other stuff.
-----Original Message----- X-from: jcarroll-at-murraystate.edu [mailto:jcarroll-at-murraystate.edu] Sent: Wednesday, January 25, 2017 3:41 PM To: Monson, Frederick {FMonson-at-wcupa.edu}
I am a relatively inexperienced TEM tech and am having problems with my PTA. Each time I make fresh PTA, I end up with an image full of stain blobs. Obviously this is useless. I have tried purchasing fresh PTA, but that didn't make any difference. I have also used BSA, which helps some, but not a lot. Help! Any ideas are welcome.
Jami
Jami Carroll Lab Tech II - Virology Breathitt Veterinary Center Hopkinsville, KY 270-886-3959 jcarroll-at-murraystate.edu
==============================Original Headers============================== 3, 41 -- From jcarroll-at-murraystate.edu Wed Jan 25 14:29:39 2017 3, 41 -- Received: from isenguard1.murraystate.edu (isenguard1.murraystate.edu [216.249.159.66]) 3, 41 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0PKTbsW003097 3, 41 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Jan 2017 14:29:38 -0600 3, 41 -- Received: (qmail 9596 invoked by uid 514); 25 Jan 2017 14:36:00 -0600 3, 41 -- Received: from mail-io0-f200.google.com (209.85.223.200) 3, 41 -- by isenguard1.murraystate.edu with AES128-SHA encrypted SMTP; 25 Jan 2017 14:36:00 -0600 3, 41 -- Received: by mail-io0-f200.google.com with SMTP id j13so22886139iod.6 3, 41 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Jan 2017 12:34:48 -0800 (PST) 3, 41 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 41 -- d=murraystate.edu; s=google; 3, 41 -- h=from:mime-version:thread-index:date:message-id:subject:to; 3, 41 -- bh=TbOf5vsspNPFILyzcNL/dvwC+HvU+9H0+31YCGmgl/E=; 3, 41 -- b=WZ6VhdSIqrdc5oOkZa7X1Rmj+5334BWEDO33FudPIC3aJovIJXOWOrdWfyhmfHMaUJ 3, 41 -- 3lyo64W+gSTWA9EkIT/48KPnSDGAbk8beOzVQcqGvxuV5ygp/Q1N6BEuM5EHhIrlpQSb 3, 41 -- Ls9EbdTtw7y4Kjg32hUlIzYyg9SCW9KYjMLsc= 3, 41 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 41 -- d=1e100.net; s=20161025; 3, 41 -- h=x-gm-message-state:from:mime-version:thread-index:date:message-id 3, 41 -- :subject:to; 3, 41 -- bh=TbOf5vsspNPFILyzcNL/dvwC+HvU+9H0+31YCGmgl/E=; 3, 41 -- b=Jn648cp78H0CzTgjnBLa8maTkzAHTTnAjlUfEZ/A0n8eozRDZ1HZRy2ReC6H6SPo56 3, 41 -- bprdzy1+8O1V1+5s6Fi3E13J0Ad4Hrgfm5OviMFW3WOqsc3bBvxpWgTf4DGwsVfDnha+ 3, 41 -- QgpdEVd3DTrzyhlnkoYN0PjkunxisCYDWKWSyMEg2Wx1W613he2omkJzb2+PzIAJPdCJ 3, 41 -- +Xcmtjb26k25Y2bYIF/KvVqah+R1NAqLabeL5mHeoUhTzzRz96TTTutM1UWcU298LDCn 3, 41 -- 6qBeB5JCKN3XtvNe7W+qQq0b3+5GWoPEK0MUR610ZX8KhzPMlowdnpl+zMM+058oyUhf 3, 41 -- oVQQ== 3, 41 -- X-Gm-Message-State: AIkVDXJEKzPrp/T8tSiVuZJre5xfryNuP0448Sa+UFXWZ4MC9fvb9dc6VeOi4qI+6KSXlDOH48VQv+wBIH8OkDdlGeluC39/fcXLBOUu0V7jOSAG6EmBQfFpOu2Ju50ZUGfZAWmRG2h7rjRFAz3WXU7rbu6r5YzvzAI= 3, 41 -- X-Received: by 10.107.10.24 with SMTP id u24mr34419759ioi.94.1485376487879; 3, 41 -- Wed, 25 Jan 2017 12:34:47 -0800 (PST) 3, 41 -- X-Received: by 10.107.10.24 with SMTP id u24mr34419752ioi.94.1485376487758; 3, 41 -- Wed, 25 Jan 2017 12:34:47 -0800 (PST) 3, 41 -- From: Jami Carroll {jcarroll-at-murraystate.edu} 3, 41 -- MIME-Version: 1.0 3, 41 -- X-Mailer: Microsoft Outlook 15.0 3, 41 -- Thread-Index: AdJ3SlzXhP0ZYrc6S9+CcE8EBSgGPw== 3, 41 -- Date: Wed, 25 Jan 2017 14:34:46 -0600 3, 41 -- Message-ID: {316b19cf1154b769f967a2c0a91d4a27-at-mail.gmail.com} 3, 41 -- Subject: TEM negative staining issues 3, 41 -- To: Microscopy-at-microscopy.com 3, 41 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
==============================Original Headers============================== 24, 41 -- From FMonson-at-wcupa.edu Wed Jan 25 15:42:06 2017 24, 41 -- Received: from MX01.WCUPA.EDU (mx01.wcupa.edu [144.26.63.2]) 24, 41 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0PLg6JN026497 24, 41 -- for {microscopy-at-microscopy.com} ; Wed, 25 Jan 2017 15:42:06 -0600 24, 41 -- X-ASG-Debug-ID: 1485380837-08911c2a5835b0a0001-4CH8be 24, 41 -- Received: from WCUXCHP01.PASSHE.LCL ([144.26.2.137]) by MX01.WCUPA.EDU with ESMTP id qF5wgjG9ogHROS2a (version=TLSv1.2 cipher=ECDHE-RSA-AES256-SHA384 bits=256 verify=NO); Wed, 25 Jan 2017 16:47:17 -0500 (EST) 24, 41 -- X-Barracuda-Envelope-From: FMonson-at-wcupa.edu 24, 41 -- X-Barracuda-Apparent-Source-IP: 144.26.2.137 24, 41 -- X-ASG-Whitelist: Client 24, 41 -- Received: from WCUXCHP08.PASSHE.LCL (144.26.2.144) by WCUXCHP01.PASSHE.LCL 24, 41 -- (144.26.2.137) with Microsoft SMTP Server (TLS) id 15.0.1178.4; Wed, 25 Jan 24, 41 -- 2017 16:46:45 -0500 24, 41 -- Received: from WCUXCHP08.PASSHE.LCL ([144.26.2.144]) by WCUXCHP08.PASSHE.LCL 24, 41 -- ([144.26.2.144]) with mapi id 15.00.1178.000; Wed, 25 Jan 2017 16:46:45 -0500 24, 41 -- From: "Monson, Frederick" {FMonson-at-wcupa.edu} 24, 41 -- To: "jcarroll-at-murraystate.edu" {jcarroll-at-murraystate.edu} 24, 41 -- CC: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 24, 41 -- Subject: RE: [Microscopy] TEM negative staining issues 24, 41 -- Thread-Topic: [Microscopy] TEM negative staining issues 24, 41 -- X-ASG-Orig-Subj: RE: [Microscopy] TEM negative staining issues 24, 41 -- Thread-Index: AQHSd0v9UudivfJbB0253DI8Or0XKaFJr4Dw 24, 41 -- Date: Wed, 25 Jan 2017 21:46:44 +0000 24, 41 -- Message-ID: {d3698c21c4ed40f0989836c0a643a53d-at-WCUXCHP08.PASSHE.LCL} 24, 41 -- References: {201701252040.v0PKexdF013610-at-microscopy.com} 24, 41 -- In-Reply-To: {201701252040.v0PKexdF013610-at-microscopy.com} 24, 41 -- Accept-Language: en-US 24, 41 -- Content-Language: en-US 24, 41 -- X-MS-Has-Attach: 24, 41 -- X-MS-TNEF-Correlator: 24, 41 -- x-ms-exchange-transport-fromentityheader: Hosted 24, 41 -- x-originating-ip: [144.26.2.59] 24, 41 -- Content-Type: text/plain; charset="us-ascii" 24, 41 -- MIME-Version: 1.0 24, 41 -- X-Barracuda-Connect: UNKNOWN[144.26.2.137] 24, 41 -- X-Barracuda-Start-Time: 1485380837 24, 41 -- X-Barracuda-Encrypted: ECDHE-RSA-AES256-SHA384 24, 41 -- X-Barracuda-URL: https://SPAMCONTROL.WCUPA.EDU:443/cgi-mod/mark.cgi 24, 41 -- X-Virus-Scanned: by bsmtpd at WCUPA.EDU 24, 41 -- X-Barracuda-BRTS-Status: 1 24, 41 -- Content-Transfer-Encoding: 8bit 24, 41 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v0PLg6JN026497 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both SLC6-at-Lehigh.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: SLC6-at-Lehigh.edu Name: Sharon Coe
Organization: Lehigh University
Title-Subject: [Filtered] Lehigh Microscopy School 2017
Message: Now accepting registrations for the 47th annual Lehigh Microscopy School which will be held on the campus of Lehigh University, Bethlehem, PA, June 4-9, 2017. All courses, lecturers, and instrument suppliers will be together for what promises to be a phenomenal week! Course offerings include: SEM and X-ray Microanalysis Introduction to SEM and EDS for the New Operator Focused Ion Beam Instrumentation and Applications Problem Solving: Interpretation and Analysis of SEM/EDS/EBSD Data Quantitative X-ray Microanalysis Problem Solving Using EDS and WDS Techniques Scanning Transmission Electron Microscopy: From Fundamentals to Advanced Applications. Register and pay in full by April 14 for an early bird discount!
Contact: Sharon Coe (sharon.coe-at-Lehigh.edu or 610-758-5133). See www.Lehigh.edu/microscopy for registration form, prices, and details about courses, lecturers, and instrument suppliers.
Scholarships available for full-time graduate students.
Login Host: 128.180.54.31 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
From rotator-at-mg-style.cn Wed Jan 25 23:29:13 2017 Return-Path: {rotator-at-mg-style.cn} Received: from mg-style.cn ([43.225.199.130]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v0Q5TBkA017975 for {microscopylistserverarchive6-at-microscopy.com} ; Wed, 25 Jan 2017 23:29:12 -0600 DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; s=key79; d=mg-style.cn; h=Message-ID:Date:Reply-To:From:User-Agent:MIME-Version:To:Subject:Content-Type:Content-Transfer-Encoding; b=ZZCGIFIsrQT++VG8fU4afLjmO34GlRzpL+0iy9cnSpBdogAFeDfpwruXFb6SiwpvY0JYWit9+IA6HA11F+uXaJb0k3QO345/Fl3STl5FYhsQomQ/i636BCasolH0prCal7UUbIy+rXeuSmpm3GoHPgoXVJWcttIw/YfkEnuLv0I=; Message-ID: {075C9ED5.7ECDC7CF-at-mg-style.cn}
Dear All, i would like to ask for your recommendation for a temperature logger. Probably a reasonable sensitivity, acuracy and precision and is able to monitor live using the TEM pc. I am not sure if such system will interfere any performance of the TEM. If you encounter such problem, is it possible to share your experience? I do know some logger has wifi/bluetooth capability, will this interfere the TEM performances well? whiCurrently we have a temperature logger but its not live and we have to extract the data out from time to time which is very troublesome.
Cheers, Yee Yan, Tay FACTS Lab NTU Singapore
==============================Original Headers============================== 2, 29 -- From rongchigram79-at-yahoo.com.sg Thu Jan 26 02:46:34 2017 2, 29 -- Received: from nm11-vm7.bullet.mail.sg3.yahoo.com (nm11-vm7.bullet.mail.sg3.yahoo.com [106.10.148.246]) 2, 29 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0Q8kWah020307 2, 29 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Jan 2017 02:46:33 -0600 2, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com.sg; s=s2048; t=1485420703; bh=6qN0PhRcJB09HCGFUyxPVhRd9guTg7iqD/EvlCLXJnc=; h=Date:From:Reply-To:To:Subject:References:From:Subject; b=c6Tt98oAxObfHMm7NLdKppiPxSprMYMCNhxNxQTkQv+xr75cFypmnoyMLKl8r30x18LEw9GLUhMnPW82J25ERJs/ajq1vyc1WlPmtfvPjCS8k4OlIJbNZ3quSlKM6Od4dO7HyIKBzYURwOBFQeRMlFve0yWRJawwZtmqO5UDxGPXQvEuaH6evssrmmhauYKeQTMn77OFwg3MGY7e8Brz6J9WW6DmXVi2B1pLUxJLUO2mEKl3ONcYuPAwyCFVlyCQoiWKi0/iyq/tm5VT2OYwdXQaQysl/no1tEdu992hoP8+mgck+PRFCfnAgPVWEQi1UhQiGTDysMMfulSsOUn2WA== 2, 29 -- Received: from [106.10.166.120] by nm11.bullet.mail.sg3.yahoo.com with NNFMP; 26 Jan 2017 08:51:43 -0000 2, 29 -- Received: from [106.10.151.250] by tm9.bullet.mail.sg3.yahoo.com with NNFMP; 26 Jan 2017 08:51:43 -0000 2, 29 -- Received: from [127.0.0.1] by omp1021.mail.sg3.yahoo.com with NNFMP; 26 Jan 2017 08:51:43 -0000 2, 29 -- X-Yahoo-Newman-Property: ymail-3 2, 29 -- X-Yahoo-Newman-Id: 394027.14600.bm-at-omp1021.mail.sg3.yahoo.com 2, 29 -- X-YMail-OSG: T0dUrMgVM1n6BVCBg3fxDjF5_oRmAxZT6IbMR7dm9HNOUedWZEnHbUKQk1J9kcB 2, 29 -- gvD6_0N0LbFHB20r4LPwA3jRlCbL1ChtHEJRgE2xSH2zYxjhFoYLGyMR3iQ7A2t1Jv.Wvs1LU.oW 2, 29 -- f._lJX8Zn1IsrG78tyRBPMDYndQYUl738yddERkQ4BCSJIXemHd2BVMGQGO74NOAWhwqhfWDck4Q 2, 29 -- 2ERUrDJdT0NOY8LrPgQIV7_T17Czr68KLBW2AdmAxu8y9romJ3W86r1ZZyO7m68VN4H8JUOXPjZ4 2, 29 -- fPbqB9et10u_YcROp6cw5MKXIuZHjEOeeiZJDqgDDFePjah7mBM26amiwMmaTzdMChJaJCtag4Fb 2, 29 -- cD87sLIE4.rkQe7brzkrpB3vZUz2kOdUcd9eR4qNjguBOmX4wdoWCeAz67M.blc0b8i9czGQbt_z 2, 29 -- OTYSbAyRzz1HnoeHLfWA7yh_SVKjzMPItsWYOD5hjD4JONHi4QTXTWoz5f.IkXhMMePMV1xDsOdI 2, 29 -- savgutwlmG_GjZL..H5GIkLrK6SsGUnDzQgW_EYMZeOc3zg-- 2, 29 -- Received: from jws600002.mail.sg3.yahoo.com by sendmailws118.mail.sg3.yahoo.com; Thu, 26 Jan 2017 08:51:42 +0000; 1485420702.563 2, 29 -- Date: Thu, 26 Jan 2017 08:51:42 +0000 (UTC) 2, 29 -- From: YY YY {rongchigram79-at-yahoo.com.sg} 2, 29 -- Reply-To: YY YY {rongchigram79-at-yahoo.com.sg} 2, 29 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 2, 29 -- Message-ID: {1775741231.1160911.1485420702386-at-mail.yahoo.com} 2, 29 -- Subject: Temperature Logger to Recommend 2, 29 -- MIME-Version: 1.0 2, 29 -- Content-Type: text/plain; charset=UTF-8 2, 29 -- Content-Transfer-Encoding: 7bit 2, 29 -- References: {1775741231.1160911.1485420702386.ref-at-mail.yahoo.com} ==============================End of - Headers==============================
you may want to talk to Guenter Resch of Nexperion. He might be able to help. His email address: guenter.resch-at-nexperion.net
Cheers,
Jan Leunissen
} On 26/01/2017, at 21:46, rongchigram79-at-yahoo.com.sg wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear All, i would like to ask for your recommendation for a temperature logger. Probably a reasonable sensitivity, acuracy and precision and is able to monitor live using the TEM pc. I am not sure if such system will interfere any performance of the TEM. If you encounter such problem, is it possible to share your experience? I do know some logger has wifi/bluetooth capability, will this interfere the TEM performances well? whiCurrently we have a temperature logger but its not live and we have to extract the data out from time to time which is very troublesome. } } Cheers, } Yee Yan, Tay } FACTS Lab } NTU } Singapore } } ==============================Original Headers============================== } 2, 29 -- From rongchigram79-at-yahoo.com.sg Thu Jan 26 02:46:34 2017 } 2, 29 -- Received: from nm11-vm7.bullet.mail.sg3.yahoo.com (nm11-vm7.bullet.mail.sg3.yahoo.com [106.10.148.246]) } 2, 29 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0Q8kWah020307 } 2, 29 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Jan 2017 02:46:33 -0600 } 2, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com.sg; s=s2048; t=1485420703; bh=6qN0PhRcJB09HCGFUyxPVhRd9guTg7iqD/EvlCLXJnc=; h=Date:From:Reply-To:To:Subject:References:From:Subject; b=c6Tt98oAxObfHMm7NLdKppiPxSprMYMCNhxNxQTkQv+xr75cFypmnoyMLKl8r30x18LEw9GLUhMnPW82J25ERJs/ajq1vyc1WlPmtfvPjCS8k4OlIJbNZ3quSlKM6Od4dO7HyIKBzYURwOBFQeRMlFve0yWRJawwZtmqO5UDxGPXQvEuaH6evssrmmhauYKeQTMn77OFwg3MGY7e8Brz6J9WW6DmXVi2B1pLUxJLUO2mEKl3ONcYuPAwyCFVlyCQoiWKi0/iyq/tm5VT2OYwdXQaQysl/no1tEdu992hoP8+mgck+PRFCfnAgPVWEQi1UhQiGTDysMMfulSsOUn2WA== } 2, 29 -- Received: from [106.10.166.120] by nm11.bullet.mail.sg3.yahoo.com with NNFMP; 26 Jan 2017 08:51:43 -0000 } 2, 29 -- Received: from [106.10.151.250] by tm9.bullet.mail.sg3.yahoo.com with NNFMP; 26 Jan 2017 08:51:43 -0000 } 2, 29 -- Received: from [127.0.0.1] by omp1021.mail.sg3.yahoo.com with NNFMP; 26 Jan 2017 08:51:43 -0000 } 2, 29 -- X-Yahoo-Newman-Property: ymail-3 } 2, 29 -- X-Yahoo-Newman-Id: 394027.14600.bm-at-omp1021.mail.sg3.yahoo.com } 2, 29 -- X-YMail-OSG: T0dUrMgVM1n6BVCBg3fxDjF5_oRmAxZT6IbMR7dm9HNOUedWZEnHbUKQk1J9kcB } 2, 29 -- gvD6_0N0LbFHB20r4LPwA3jRlCbL1ChtHEJRgE2xSH2zYxjhFoYLGyMR3iQ7A2t1Jv.Wvs1LU.oW } 2, 29 -- f._lJX8Zn1IsrG78tyRBPMDYndQYUl738yddERkQ4BCSJIXemHd2BVMGQGO74NOAWhwqhfWDck4Q } 2, 29 -- 2ERUrDJdT0NOY8LrPgQIV7_T17Czr68KLBW2AdmAxu8y9romJ3W86r1ZZyO7m68VN4H8JUOXPjZ4 } 2, 29 -- fPbqB9et10u_YcROp6cw5MKXIuZHjEOeeiZJDqgDDFePjah7mBM26amiwMmaTzdMChJaJCtag4Fb } 2, 29 -- cD87sLIE4.rkQe7brzkrpB3vZUz2kOdUcd9eR4qNjguBOmX4wdoWCeAz67M.blc0b8i9czGQbt_z } 2, 29 -- OTYSbAyRzz1HnoeHLfWA7yh_SVKjzMPItsWYOD5hjD4JONHi4QTXTWoz5f.IkXhMMePMV1xDsOdI } 2, 29 -- savgutwlmG_GjZL..H5GIkLrK6SsGUnDzQgW_EYMZeOc3zg-- } 2, 29 -- Received: from jws600002.mail.sg3.yahoo.com by sendmailws118.mail.sg3.yahoo.com; Thu, 26 Jan 2017 08:51:42 +0000; 1485420702.563 } 2, 29 -- Date: Thu, 26 Jan 2017 08:51:42 +0000 (UTC) } 2, 29 -- From: YY YY {rongchigram79-at-yahoo.com.sg} } 2, 29 -- Reply-To: YY YY {rongchigram79-at-yahoo.com.sg} } 2, 29 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} } 2, 29 -- Message-ID: {1775741231.1160911.1485420702386-at-mail.yahoo.com} } 2, 29 -- Subject: Temperature Logger to Recommend } 2, 29 -- MIME-Version: 1.0 } 2, 29 -- Content-Type: text/plain; charset=UTF-8 } 2, 29 -- Content-Transfer-Encoding: 7bit } 2, 29 -- References: {1775741231.1160911.1485420702386.ref-at-mail.yahoo.com} } ==============================End of - Headers==============================
==============================Original Headers============================== 7, 35 -- From leunissen-at-aurion.nl Thu Jan 26 03:04:45 2017 7, 35 -- Received: from nm14-vm5.access.bullet.mail.bf1.yahoo.com (nm14-vm5.access.bullet.mail.bf1.yahoo.com [216.109.115.20]) 7, 35 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0Q94i60006442 7, 35 -- for {microscopy-at-microscopy.com} ; Thu, 26 Jan 2017 03:04:45 -0600 7, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1485421797; bh=MH/TbUKNWbFVdYQai4502bsM24Lj+D9xdpYMa9OG9Jk=; h=Subject:From:In-Reply-To:Date:Cc:References:To:From:Subject; b=qJkC1rNRU9AIz+CPjXW9fwKkebRbAY/w8z73vGeDFazB1Td7JrPOawGBsAqTC27lZPIhKBfaZgzT7qL7ufLadUnJ3aMVl8dCr4/rVt1zhZdZBd5c7sGGLm3PXPXwvL9lDOwun/+IyMuer+lEDFYpkmuvclW3uX0g32lgKBOQ9BY7MO5kbZYSAFW/5WJ4q1gNW6bBZbeVbxSJRpqqgOYStehDZE/gsOnDv/BiAXQB/h4m2iOLEnRdIEj7i0m4Y2OAYYlJ0b1vSYuq4EQ+YV423ltnPjUxRdLmBgjlQkkpUxdpFJ4bvjXtHxgwrargUNuWM7L/ZbvZ/pVZuz6uavmeuw== 7, 35 -- Received: from [66.196.81.166] by nm14.access.bullet.mail.bf1.yahoo.com with NNFMP; 26 Jan 2017 09:09:57 -0000 7, 35 -- Received: from [98.138.226.241] by tm12.access.bullet.mail.bf1.yahoo.com with NNFMP; 26 Jan 2017 09:09:57 -0000 7, 35 -- Received: from [127.0.0.1] by smtp112.sbc.mail.ne1.yahoo.com with NNFMP; 26 Jan 2017 09:09:57 -0000 7, 35 -- X-Yahoo-Newman-Id: 357734.1479.bm-at-smtp112.sbc.mail.ne1.yahoo.com 7, 35 -- X-Yahoo-Newman-Property: ymail-3 7, 35 -- X-YMail-OSG: 0F1VpCEVM1nIpyLz.CM3YAYJV9VYBi9E1dByjbdxnVmvA8O 7, 35 -- tibdXHrRAJI.EPAH23y1f0daPVQcKBnktrcIP1XAVXfpl2pRJbFSquJPOk5R 7, 35 -- YJqETXq_KG5Cs0zagyx1o.E4sgbVlsmxeVcUIjlqw.yMTR92vSwl8Uz_83uM 7, 35 -- BPmtnGQcIvniVIESNvma3WiPS1aUbUxpPsufOZY7maZ59fAo.9_90kbr0WhN 7, 35 -- MFRBitUXxidgZPtRCn.9dYbK3qanp9_FQtvVsAzAZ8cNRn6LnwurRbcdYKS1 7, 35 -- iwSVZS6.tnWojLetAZSa.nTkcT9uvd7ZEfwlZ6OMNIpi3G1Ybo0hE4F1TBY4 7, 35 -- TZdiiQJcdROJ2N9NkkPUWm4tDXJ6Eijml8f3_sTbkKZHR4cBXTAWQn75FRFa 7, 35 -- bwIDBfT97AZ0vcttYNiHr.k99IseROoCBEJ8oHISfkb3f22RUY5qyg8TXtZA 7, 35 -- c6yqzQ3FmYP3fITOTcgP5wUz3av9YH_BFyCioy6wgCxs4yvFx7.hRsxxGFV5 7, 35 -- _2Q6EYo0RFQBwUpLhCbu70OUUpGl.UBfHpRkrBXm1JYYCdoIHAhTyfZ1An9k 7, 35 -- VedqP.7PnQA_bKQ-- 7, 35 -- X-Yahoo-SMTP: E1TINkOswBCOniPaNnp23qBGQpLN4KsU.cr0KdlJlWhF 7, 35 -- Subject: Re: [Microscopy] Temperature Logger to Recommend 7, 35 -- Mime-Version: 1.0 (Mac OS X Mail 9.3 \(3124\)) 7, 35 -- Content-Type: text/plain; charset=us-ascii 7, 35 -- From: Jan Leunissen {leunissen-at-aurion.nl} 7, 35 -- In-Reply-To: {201701260846.v0Q8kpIx020562-at-microscopy.com} 7, 35 -- Date: Thu, 26 Jan 2017 22:09:50 +1300 7, 35 -- Cc: microscopy-at-microscopy.com 7, 35 -- Message-Id: {9C33975F-2ABE-41B8-B3B9-67FCDAF1EBEB-at-aurion.nl} 7, 35 -- References: {201701260846.v0Q8kpIx020562-at-microscopy.com} 7, 35 -- To: rongchigram79-at-yahoo.com.sg 7, 35 -- X-Mailer: Apple Mail (2.3124) 7, 35 -- Content-Transfer-Encoding: 8bit 7, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v0Q94i60006442 ==============================End of - Headers==============================
I have used Extech RH520A temperature/humidity data logger in FIB room and was very pleased with it in all respects. Initial setup required some programming, but features and display/download capabilities were excellent:
http://www.extech.com/display/?id=14702
Valery Ray www.linkedin.com/in/valeryray {http://www.linkedin.com/in/valeryray} ============================== PBS&T, MEO Engineering Company 290 Broadway, Suite 298 Methuen, MA 01844, USA Phone: +1-978-305-0479 {tel:%2B1-978-305-0479} - leave a message Mobie: +1-978-305-0479 {tel:%2B1-978-305-0479} - leave a message E-mail: vray-at-partbeamsystech.com {mailto:vray-at-partbeamsystech.com} Web: www.partbeamsystech.com {http://www.partbeamsystech.com} Web: www.freudlabs.com {http://www.freudlabs.com}
On 1/26/2017 4:06 AM, leunissen-at-aurion.nl wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe --http://www.microscopy.com/MicroscopyListserver } On-Line Helphttp://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello, } } you may want to talk to Guenter Resch of Nexperion. He might be able to help. } His email address:guenter.resch-at-nexperion.net } } Cheers, } } Jan Leunissen } } } On 26/01/2017, at 21:46,rongchigram79-at-yahoo.com.sg wrote: } } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe --http://www.microscopy.com/MicroscopyListserver } } On-Line Helphttp://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Dear All, i would like to ask for your recommendation for a temperature logger. Probably a reasonable sensitivity, acuracy and precision and is able to monitor live using the TEM pc. I am not sure if such system will interfere any performance of the TEM. If you encounter such problem, is it possible to share your experience? I do know some logger has wifi/bluetooth capability, will this interfere the TEM performances well? whiCurrently we have a temperature logger but its not live and we have to extract the data out from time to time which is very troublesome. } } } } Cheers, } } Yee Yan, Tay } } FACTS Lab } } NTU } } Singapore } } } } ==============================Original Headers============================== } } 2, 29 -- Fromrongchigram79-at-yahoo.com.sg Thu Jan 26 02:46:34 2017 } } 2, 29 -- Received: from nm11-vm7.bullet.mail.sg3.yahoo.com (nm11-vm7.bullet.mail.sg3.yahoo.com [106.10.148.246]) } } 2, 29 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0Q8kWah020307 } } 2, 29 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Jan 2017 02:46:33 -0600 } } 2, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com.sg; s=s2048; t=1485420703; bh=6qN0PhRcJB09HCGFUyxPVhRd9guTg7iqD/EvlCLXJnc=; h=Date:From:Reply-To:To:Subject:References:From:Subject; b=c6Tt98oAxObfHMm7NLdKppiPxSprMYMCNhxNxQTkQv+xr75cFypmnoyMLKl8r30x18LEw9GLUhMnPW82J25ERJs/ajq1vyc1WlPmtfvPjCS8k4OlIJbNZ3quSlKM6Od4dO7HyIKBzYURwOBFQeRMlFve0yWRJawwZtmqO5UDxGPXQvEuaH6evssrmmhauYKeQTMn77OFwg3MGY7e8Brz6J9WW6DmXVi2B1pLUxJLUO2mEKl3ONcYuPAwyCFVlyCQoiWKi0/iyq/tm5VT2OYwdXQaQysl/no1tEdu992hoP8+mgck+PRFCfnAgPVWEQi1UhQiGTDysMMfulSsOUn2WA== } } 2, 29 -- Received: from [106.10.166.120] by nm11.bullet.mail.sg3.yahoo.com with NNFMP; 26 Jan 2017 08:51:43 -0000 } } 2, 29 -- Received: from [106.10.151.250] by tm9.bullet.mail.sg3.yahoo.com with NNFMP; 26 Jan 2017 08:51:43 -0000 } } 2, 29 -- Received: from [127.0.0.1] by omp1021.mail.sg3.yahoo.com with NNFMP; 26 Jan 2017 08:51:43 -0000 } } 2, 29 -- X-Yahoo-Newman-Property: ymail-3 } } 2, 29 -- X-Yahoo-Newman-Id:394027.14600.bm-at-omp1021.mail.sg3.yahoo.com } } 2, 29 -- X-YMail-OSG: T0dUrMgVM1n6BVCBg3fxDjF5_oRmAxZT6IbMR7dm9HNOUedWZEnHbUKQk1J9kcB } } 2, 29 -- gvD6_0N0LbFHB20r4LPwA3jRlCbL1ChtHEJRgE2xSH2zYxjhFoYLGyMR3iQ7A2t1Jv.Wvs1LU.oW } } 2, 29 -- f._lJX8Zn1IsrG78tyRBPMDYndQYUl738yddERkQ4BCSJIXemHd2BVMGQGO74NOAWhwqhfWDck4Q } } 2, 29 -- 2ERUrDJdT0NOY8LrPgQIV7_T17Czr68KLBW2AdmAxu8y9romJ3W86r1ZZyO7m68VN4H8JUOXPjZ4 } } 2, 29 -- fPbqB9et10u_YcROp6cw5MKXIuZHjEOeeiZJDqgDDFePjah7mBM26amiwMmaTzdMChJaJCtag4Fb } } 2, 29 -- cD87sLIE4.rkQe7brzkrpB3vZUz2kOdUcd9eR4qNjguBOmX4wdoWCeAz67M.blc0b8i9czGQbt_z } } 2, 29 -- OTYSbAyRzz1HnoeHLfWA7yh_SVKjzMPItsWYOD5hjD4JONHi4QTXTWoz5f.IkXhMMePMV1xDsOdI } } 2, 29 -- savgutwlmG_GjZL..H5GIkLrK6SsGUnDzQgW_EYMZeOc3zg-- } } 2, 29 -- Received: from jws600002.mail.sg3.yahoo.com by sendmailws118.mail.sg3.yahoo.com; Thu, 26 Jan 2017 08:51:42 +0000; 1485420702.563 } } 2, 29 -- Date: Thu, 26 Jan 2017 08:51:42 +0000 (UTC) } } 2, 29 -- From: YY YY {rongchigram79-at-yahoo.com.sg} } } 2, 29 -- Reply-To: YY YY {rongchigram79-at-yahoo.com.sg} } } 2, 29 -- To:"Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} } } 2, 29 -- Message-ID: {1775741231.1160911.1485420702386-at-mail.yahoo.com} } } 2, 29 -- Subject: Temperature Logger to Recommend } } 2, 29 -- MIME-Version: 1.0 } } 2, 29 -- Content-Type: text/plain; charset=UTF-8 } } 2, 29 -- Content-Transfer-Encoding: 7bit } } 2, 29 -- References: {1775741231.1160911.1485420702386.ref-at-mail.yahoo.com} } } ==============================End of - Headers============================== } } } ==============================Original Headers============================== } 7, 35 -- Fromleunissen-at-aurion.nl Thu Jan 26 03:04:45 2017 } 7, 35 -- Received: from nm14-vm5.access.bullet.mail.bf1.yahoo.com (nm14-vm5.access.bullet.mail.bf1.yahoo.com [216.109.115.20]) } 7, 35 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0Q94i60006442 } 7, 35 -- for {microscopy-at-microscopy.com} ; Thu, 26 Jan 2017 03:04:45 -0600 } 7, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1485421797; bh=MH/TbUKNWbFVdYQai4502bsM24Lj+D9xdpYMa9OG9Jk=; h=Subject:From:In-Reply-To:Date:Cc:References:To:From:Subject; b=qJkC1rNRU9AIz+CPjXW9fwKkebRbAY/w8z73vGeDFazB1Td7JrPOawGBsAqTC27lZPIhKBfaZgzT7qL7ufLadUnJ3aMVl8dCr4/rVt1zhZdZBd5c7sGGLm3PXPXwvL9lDOwun/+IyMuer+lEDFYpkmuvclW3uX0g32lgKBOQ9BY7MO5kbZYSAFW/5WJ4q1gNW6bBZbeVbxSJRpqqgOYStehDZE/gsOnDv/BiAXQB/h4m2iOLEnRdIEj7i0m4Y2OAYYlJ0b1vSYuq4EQ+YV423ltnPjUxRdLmBgjlQkkpUxdpFJ4bvjXtHxgwrargUNuWM7L/ZbvZ/pVZuz6uavmeuw== } 7, 35 -- Received: from [66.196.81.166] by nm14.access.bullet.mail.bf1.yahoo.com with NNFMP; 26 Jan 2017 09:09:57 -0000 } 7, 35 -- Received: from [98.138.226.241] by tm12.access.bullet.mail.bf1.yahoo.com with NNFMP; 26 Jan 2017 09:09:57 -0000 } 7, 35 -- Received: from [127.0.0.1] by smtp112.sbc.mail.ne1.yahoo.com with NNFMP; 26 Jan 2017 09:09:57 -0000 } 7, 35 -- X-Yahoo-Newman-Id:357734.1479.bm-at-smtp112.sbc.mail.ne1.yahoo.com } 7, 35 -- X-Yahoo-Newman-Property: ymail-3 } 7, 35 -- X-YMail-OSG: 0F1VpCEVM1nIpyLz.CM3YAYJV9VYBi9E1dByjbdxnVmvA8O } 7, 35 -- tibdXHrRAJI.EPAH23y1f0daPVQcKBnktrcIP1XAVXfpl2pRJbFSquJPOk5R } 7, 35 -- YJqETXq_KG5Cs0zagyx1o.E4sgbVlsmxeVcUIjlqw.yMTR92vSwl8Uz_83uM } 7, 35 -- BPmtnGQcIvniVIESNvma3WiPS1aUbUxpPsufOZY7maZ59fAo.9_90kbr0WhN } 7, 35 -- MFRBitUXxidgZPtRCn.9dYbK3qanp9_FQtvVsAzAZ8cNRn6LnwurRbcdYKS1 } 7, 35 -- iwSVZS6.tnWojLetAZSa.nTkcT9uvd7ZEfwlZ6OMNIpi3G1Ybo0hE4F1TBY4 } 7, 35 -- TZdiiQJcdROJ2N9NkkPUWm4tDXJ6Eijml8f3_sTbkKZHR4cBXTAWQn75FRFa } 7, 35 -- bwIDBfT97AZ0vcttYNiHr.k99IseROoCBEJ8oHISfkb3f22RUY5qyg8TXtZA } 7, 35 -- c6yqzQ3FmYP3fITOTcgP5wUz3av9YH_BFyCioy6wgCxs4yvFx7.hRsxxGFV5 } 7, 35 -- _2Q6EYo0RFQBwUpLhCbu70OUUpGl.UBfHpRkrBXm1JYYCdoIHAhTyfZ1An9k } 7, 35 -- VedqP.7PnQA_bKQ-- } 7, 35 -- X-Yahoo-SMTP: E1TINkOswBCOniPaNnp23qBGQpLN4KsU.cr0KdlJlWhF } 7, 35 -- Subject: Re: [Microscopy] Temperature Logger to Recommend } 7, 35 -- Mime-Version: 1.0 (Mac OS X Mail 9.3 \(3124\)) } 7, 35 -- Content-Type: text/plain; charset=us-ascii } 7, 35 -- From: Jan Leunissen {leunissen-at-aurion.nl} } 7, 35 -- In-Reply-To: {201701260846.v0Q8kpIx020562-at-microscopy.com} } 7, 35 -- Date: Thu, 26 Jan 2017 22:09:50 +1300 } 7, 35 -- Cc:microscopy-at-microscopy.com } 7, 35 -- Message-Id: {9C33975F-2ABE-41B8-B3B9-67FCDAF1EBEB-at-aurion.nl} } 7, 35 -- References: {201701260846.v0Q8kpIx020562-at-microscopy.com} } 7, 35 -- To:rongchigram79-at-yahoo.com.sg } 7, 35 -- X-Mailer: Apple Mail (2.3124) } 7, 35 -- Content-Transfer-Encoding: 8bit } 7, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v0Q94i60006442 } ==============================End of - Headers==============================
==============================Original Headers============================== 7, 35 -- From vray-at-partbeamsystech.com Thu Jan 26 10:45:10 2017 7, 35 -- Received: from nm4.bullet.mail.bf1.yahoo.com (nm4.bullet.mail.bf1.yahoo.com [98.139.212.163]) 7, 35 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0QGjA3X006147 7, 35 -- for {microscopy-at-microscopy.com} ; Thu, 26 Jan 2017 10:45:10 -0600 7, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1485449424; bh=ZarplFmV6X95X4w09r2j5hUu8Zysmw9RP1W6eh2GHsw=; h=Subject:References:To:From:Date:In-Reply-To:From:Subject; b=kztWGNC4jYIuPZlfVhU5vXQEA06QpQRUQA+83eDHCy6p9907vevioA+4qdirVMEwXJthxtxZzF3RjeabMHUO1vsM6oiopHSj8WIDNdusBYuSFVmGqxcO9Gd+pksDCTY1ab08oVnP24ybSdI77d3PnVwXGBNAM8IK5hLinz+RoZIfhF5RacLKMjtdfa24+y4s5JA3eqO5e8oOPCNm8vPzGxlPFdonIxjM6MzttWiFqtqiYKuZwEdEymHl4LBVjk9Dge2qgN+a191hBKIg0mKy4VmCSBCarR00NRsif4yzvhr6n0fExC5mP6BT3YQoX0mZ9/DhNpkgSQHLWj3vca2euQ== 7, 35 -- Received: from [66.196.81.172] by nm4.bullet.mail.bf1.yahoo.com with NNFMP; 26 Jan 2017 16:50:24 -0000 7, 35 -- Received: from [98.139.211.194] by tm18.bullet.mail.bf1.yahoo.com with NNFMP; 26 Jan 2017 16:50:24 -0000 7, 35 -- Received: from [127.0.0.1] by smtp203.mail.bf1.yahoo.com with NNFMP; 26 Jan 2017 16:50:24 -0000 7, 35 -- X-Yahoo-Newman-Id: 235263.13354.bm-at-smtp203.mail.bf1.yahoo.com 7, 35 -- X-Yahoo-Newman-Property: ymail-3 7, 35 -- X-YMail-OSG: SJKkmnoVM1lLqQ4_7M4148Lbf5.GxlZkCuMZ7z9l38hf3_H 7, 35 -- GD1_2fKEfkgSHD7qmS451Az.XhO6lZ4hxiCDEtLBAAFh_WOIS8651jtNbI5_ 7, 35 -- hi1z1c6Mk.1Kcs.omiPN7qIgSTKJbkgjRPGqTWzz4gZY3MrAw6kHDhUkVC1O 7, 35 -- pK5QCgq0uxr0UorPNXnaEHGU_STt8RcL27vSIaQniydE_4gGBmQnuw8KmtAZ 7, 35 -- UEk5z9QPZxdoTvw0ClZ2XPoy_qCflk4qycTB28kXyzxRIR7EQU1_Wo4heKcz 7, 35 -- R872kTjVqs83egQurXLSQnux5Y33.6yyFha8sYKEkoZCtZ2E.8bD7FRlv3nZ 7, 35 -- 7_PvlMcHvXvNJWLQ_t0E.Hct.vz9Vza9q07TBIbQOVz_R6IFT_A53W4_cJRn 7, 35 -- XAOcYQ7SehnnmuBKpq8E.1wim9ER6g1kX9OFNu2mup9x8jJDEG4bKnQKNHB3 7, 35 -- vS9994VG0ma5q63GmCetzHFqZQpz.jp3kp6h8yXtittsmhBb3itbmC6z8UBB 7, 35 -- WxGyS7ib3ld_yKcn4D3.a55XKKJqJt_8CE.5e1iMDnFiN3NkjPDccPG4Ov4W 7, 35 -- kAq4bz_0p 7, 35 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 7, 35 -- Subject: Fwd: Re: [Microscopy] Re: Temperature Logger to Recommend 7, 35 -- References: {7344f3be-78ec-94cf-a873-36c6ee5bfb32-at-partbeamsystech.com} 7, 35 -- To: microscopy-at-microscopy.com 7, 35 -- From: Valery Ray {vray-at-partbeamsystech.com} 7, 35 -- X-Forwarded-Message-Id: {7344f3be-78ec-94cf-a873-36c6ee5bfb32-at-partbeamsystech.com} 7, 35 -- Message-ID: {3b8592c3-88df-bd28-841f-0497a4a7eb74-at-partbeamsystech.com} 7, 35 -- Date: Thu, 26 Jan 2017 11:48:01 -0500 7, 35 -- User-Agent: Mozilla/5.0 (Windows NT 10.0; WOW64; rv:45.0) Gecko/20100101 7, 35 -- Thunderbird/45.6.0 7, 35 -- MIME-Version: 1.0 7, 35 -- In-Reply-To: {7344f3be-78ec-94cf-a873-36c6ee5bfb32-at-partbeamsystech.com} 7, 35 -- Content-Type: text/plain; charset=windows-1252; format=flowed 7, 35 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Tel/fax +30 210 8957677 mobile +30 6945 107477 www.eikonika.netwww.aim.cat *************************************
==============================Original Headers============================== 8, 20 -- From eikonika-at-otenet.gr Fri Jan 27 03:23:23 2017 8, 20 -- Received: from chimaera.otenet.gr (smtp-out32.otenet.gr [83.235.69.32]) 8, 20 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0R9NMZg031024 8, 20 -- for {microscopy-at-microscopy.com} ; Fri, 27 Jan 2017 03:23:22 -0600 8, 20 -- Received: from ozymandias (ppp-2-87-177-161.home.otenet.gr [2.87.177.161]) 8, 20 -- by chimaera.otenet.gr (ESMTP) with ESMTPSA 8, 20 -- for {microscopy-at-microscopy.com} ; Fri, 27 Jan 2017 11:28:36 +0200 (EET) 8, 20 -- From: "Yorgos Nikas" {eikonika-at-otenet.gr} 8, 20 -- To: {microscopy-at-microscopy.com} 8, 20 -- Subject: Jeol 5600LV strip aperture sizes 8, 20 -- Date: Fri, 27 Jan 2017 11:28:37 +0200 8, 20 -- Message-ID: {000301d2787f$bd396280$37ac2780$-at-otenet.gr} 8, 20 -- MIME-Version: 1.0 8, 20 -- Content-Type: text/plain; 8, 20 -- charset="iso-8859-1" 8, 20 -- X-Mailer: Microsoft Outlook 14.0 8, 20 -- Thread-Index: AdJ4f10Kl1+ws/8YRteDf2k9m9yX8w== 8, 20 -- Content-Language: en-us 8, 20 -- Content-Transfer-Encoding: 8bit 8, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v0R9NMZg031024 ==============================End of - Headers==============================
From annepenn4-at-gmail.com Fri Jan 27 06:05:02 2017 Return-Path: {annepenn4-at-gmail.com} Received: from gmail.com ([61.76.233.68]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v0RC50j2030377 for {microscopylistserverarchive6-at-microscopy.com} ; Fri, 27 Jan 2017 06:05:01 -0600 Message-ID: {7E9065EF.1F21205C-at-gmail.com}
} I recommend a temperature sensor from Vernier.com. Their line of Go Direct sensors connect to a laptop by USB, are dirt cheap ($39 for the one I have), and come with a very nice free software package (I have never seen it crash!) for recording your measurements. I see now they have options for wireless with iOS devices. Have fun! } } } } John Mardinly, Ph.D., P.E., Retired ASU } } } } } On Jan 26, 2017, at 1:59 AM, rongchigram79-at-yahoo.com.sg wrote: } } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.com_MicroscopyListserver&d=DQIBAg&c=AGbYxfJbXK67KfXyGqyv2Ejiz41FqQuZFk4A-1IxfAU&r=MAuGvnWTcVQkxORgQD0QS50ZicPM3Nw-61ygSK-LNEQ&m=w7SxKtx8upRjcBdFpASg01gW7K2Na0pxXE9_phnExns&s=3kp26tRvY-UtqU1X55VcwWuqjnnEqOcxRc3Jhq-UwKw&e= } } On-Line Help https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.com_MicroscopyListserver_FAQ.html&d=DQIBAg&c=AGbYxfJbXK67KfXyGqyv2Ejiz41FqQuZFk4A-1IxfAU&r=MAuGvnWTcVQkxORgQD0QS50ZicPM3Nw-61ygSK-LNEQ&m=w7SxKtx8upRjcBdFpASg01gW7K2Na0pxXE9_phnExns&s=WCAC1lAnxktP17l4ddVAVdqEv21AGNCZxrUNpilB-XM&e= } } ---------------------------------------------------------------------------- } } } } Dear All, i would like to ask for your recommendation for a temperature logger. Probably a reasonable sensitivity, acuracy and precision and is able to monitor live using the TEM pc. I am not sure if such system will interfere any performance of the TEM. If you encounter such problem, is it possible to share your experience? I do know some logger has wifi/bluetooth capability, will this interfere the TEM performances well? whiCurrently we have a temperature logger but its not live and we have to extract the data out from time to time which is very troublesome. } } } } Cheers, } } Yee Yan, Tay } } FACTS Lab } } NTU } } Singapore } } } } ==============================Original Headers============================== } } 2, 29 -- From rongchigram79-at-yahoo.com.sg Thu Jan 26 02:46:34 2017 } } 2, 29 -- Received: from nm11-vm7.bullet.mail.sg3.yahoo.com (nm11-vm7.bullet.mail.sg3.yahoo.com [106.10.148.246]) } } 2, 29 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0Q8kWah020307 } } 2, 29 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Jan 2017 02:46:33 -0600 } } 2, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com.sg; s=s2048; t=1485420703; bh=6qN0PhRcJB09HCGFUyxPVhRd9guTg7iqD/EvlCLXJnc=; h=Date:From:Reply-To:To:Subject:References:From:Subject; b=c6Tt98oAxObfHMm7NLdKppiPxSprMYMCNhxNxQTkQv+xr75cFypmnoyMLKl8r30x18LEw9GLUhMnPW82J25ERJs/ajq1vyc1WlPmtfvPjCS8k4OlIJbNZ3quSlKM6Od4dO7HyIKBzYURwOBFQeRMlFve0yWRJawwZtmqO5UDxGPXQvEuaH6evssrmmhauYKeQTMn77OFwg3MGY7e8Brz6J9WW6DmXVi2B1pLUxJLUO2mEKl3ONcYuPAwyCFVlyCQoiWKi0/iyq/tm5VT2OYwdXQaQysl/no1tEdu992hoP8+mgck+PRFCfnAgPVWEQi1UhQiGTDysMMfulSsOUn2WA== } } 2, 29 -- Received: from [106.10.166.120] by nm11.bullet.mail.sg3.yahoo.com with NNFMP; 26 Jan 2017 08:51:43 -0000 } } 2, 29 -- Received: from [106.10.151.250] by tm9.bullet.mail.sg3.yahoo.com with NNFMP; 26 Jan 2017 08:51:43 -0000 } } 2, 29 -- Received: from [127.0.0.1] by omp1021.mail.sg3.yahoo.com with NNFMP; 26 Jan 2017 08:51:43 -0000 } } 2, 29 -- X-Yahoo-Newman-Property: ymail-3 } } 2, 29 -- X-Yahoo-Newman-Id: 394027.14600.bm-at-omp1021.mail.sg3.yahoo.com } } 2, 29 -- X-YMail-OSG: T0dUrMgVM1n6BVCBg3fxDjF5_oRmAxZT6IbMR7dm9HNOUedWZEnHbUKQk1J9kcB } } 2, 29 -- gvD6_0N0LbFHB20r4LPwA3jRlCbL1ChtHEJRgE2xSH2zYxjhFoYLGyMR3iQ7A2t1Jv.Wvs1LU.oW } } 2, 29 -- f._lJX8Zn1IsrG78tyRBPMDYndQYUl738yddERkQ4BCSJIXemHd2BVMGQGO74NOAWhwqhfWDck4Q } } 2, 29 -- 2ERUrDJdT0NOY8LrPgQIV7_T17Czr68KLBW2AdmAxu8y9romJ3W86r1ZZyO7m68VN4H8JUOXPjZ4 } } 2, 29 -- fPbqB9et10u_YcROp6cw5MKXIuZHjEOeeiZJDqgDDFePjah7mBM26amiwMmaTzdMChJaJCtag4Fb } } 2, 29 -- cD87sLIE4.rkQe7brzkrpB3vZUz2kOdUcd9eR4qNjguBOmX4wdoWCeAz67M.blc0b8i9czGQbt_z } } 2, 29 -- OTYSbAyRzz1HnoeHLfWA7yh_SVKjzMPItsWYOD5hjD4JONHi4QTXTWoz5f.IkXhMMePMV1xDsOdI } } 2, 29 -- savgutwlmG_GjZL..H5GIkLrK6SsGUnDzQgW_EYMZeOc3zg-- } } 2, 29 -- Received: from jws600002.mail.sg3.yahoo.com by sendmailws118.mail.sg3.yahoo.com; Thu, 26 Jan 2017 08:51:42 +0000; 1485420702.563 } } 2, 29 -- Date: Thu, 26 Jan 2017 08:51:42 +0000 (UTC) } } 2, 29 -- From: YY YY {rongchigram79-at-yahoo.com.sg} } } 2, 29 -- Reply-To: YY YY {rongchigram79-at-yahoo.com.sg} } } 2, 29 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} } } 2, 29 -- Message-ID: {1775741231.1160911.1485420702386-at-mail.yahoo.com} } } 2, 29 -- Subject: Temperature Logger to Recommend } } 2, 29 -- MIME-Version: 1.0 } } 2, 29 -- Content-Type: text/plain; charset=UTF-8 } } 2, 29 -- Content-Transfer-Encoding: 7bit } } 2, 29 -- References: {1775741231.1160911.1485420702386.ref-at-mail.yahoo.com} } } ==============================End of - Headers============================== }
==============================Original Headers============================== 4, 96 -- From John.Mardinly-at-asu.edu Fri Jan 27 10:30:47 2017 4, 96 -- Received: from bcnetw1.asu.edu (bcnetw1.asu.edu [149.169.2.71]) 4, 96 -- by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v0RGUkGD009204 4, 96 -- for {Microscopy-at-Microscopy.com} ; Fri, 27 Jan 2017 10:30:46 -0600 4, 96 -- X-ASG-Debug-ID: 1485534963-0d12c77a271103e0001-FOsErg 4, 96 -- Received: from ppnetw4.asu.edu (ppnetw4.asu.edu [10.118.52.71]) by bcnetw1.asu.edu with ESMTP id WVTnEuBXs2QOD2Jm (version=TLSv1.2 cipher=ECDHE-RSA-AES256-GCM-SHA384 bits=256 verify=NO); Fri, 27 Jan 2017 09:36:03 -0700 (MST) 4, 96 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu 4, 96 -- Received: from pps.filterd (ppnetw4.asu.edu [127.0.0.1]) 4, 96 -- by ppnetw4.asu.edu (8.16.0.17/8.16.0.17) with SMTP id v0RGZc1V021245; 4, 96 -- Fri, 27 Jan 2017 09:36:03 -0700 4, 96 -- Received: from exhubw02.asurite.ad.asu.edu (exnswvpx-10-118-52-65.inre.asu.edu [10.118.52.65]) 4, 96 -- by ppnetw4.asu.edu with ESMTP id 28893d03g7-1 4, 96 -- (version=TLSv1 cipher=ECDHE-RSA-AES256-SHA bits=256 verify=NOT); 4, 96 -- Fri, 27 Jan 2017 09:36:02 -0700 4, 96 -- Received: from NAM03-CO1-obe.outbound.protection.outlook.com (129.219.4.240) 4, 96 -- by exhubgslb.asu.edu (129.219.4.200) with Microsoft SMTP Server (TLS) id 4, 96 -- 14.3.266.1; Fri, 27 Jan 2017 09:36:02 -0700 4, 96 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 96 -- d=arizonastateu.onmicrosoft.com; s=selector1-asu-edu; 4, 96 -- h=From:Date:Subject:Message-ID:Content-Type:MIME-Version; 4, 96 -- bh=naqMZaOEeC3iExpzAGkZiww2kqIpeHTcceFQH33lSIE=; 4, 96 -- b=RoQkRKx2W8O0honOUq0kiMEoOoSFDNYyDK7BqHqvsqDO3vieIZbIin0+S6AxcCYd5ZPNfriSy0h7wSRLjDY/4juXwXMMaIqq3IDqPw0nnXOY8FkT3C2nt3GW42gp7t9ZsfJ9NRpXCSLJl1YqF2RrGdqt3w/iPOTo5a5xEYTcJxU= 4, 96 -- X-Barracuda-Effective-Source-IP: exhubw02.asurite.ad.asu.edu[129.219.4.200] 4, 96 -- X-Barracuda-Apparent-Source-IP: 129.219.4.200 4, 96 -- Received: from CY4PR06MB2904.namprd06.prod.outlook.com (10.175.118.144) by 4, 96 -- CY4PR06MB2902.namprd06.prod.outlook.com (10.175.118.142) with Microsoft SMTP 4, 96 -- Server (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_256_CBC_SHA384_P384) id 4, 96 -- 15.1.860.13; Fri, 27 Jan 2017 16:36:00 +0000 4, 96 -- Received: from CY4PR06MB2904.namprd06.prod.outlook.com ([10.175.118.144]) by 4, 96 -- CY4PR06MB2904.namprd06.prod.outlook.com ([10.175.118.144]) with mapi id 4, 96 -- 15.01.0860.023; Fri, 27 Jan 2017 16:36:00 +0000 4, 96 -- From: John Mardinly {John.Mardinly-at-asu.edu} 4, 96 -- To: "rongchigram79-at-yahoo.com.sg" {rongchigram79-at-yahoo.com.sg} , 4, 96 -- MSA Listserver 4, 96 -- {Microscopy-at-Microscopy.com} 4, 96 -- Subject: Re: [Microscopy] Temperature Logger to Recommend 4, 96 -- Thread-Topic: [Microscopy] Temperature Logger to Recommend 4, 96 -- X-ASG-Orig-Subj: Re: [Microscopy] Temperature Logger to Recommend 4, 96 -- Thread-Index: AQHSd7NBU311T3WffU6fS0JJBopyVKFMhw+AgAAAZQA= 4, 96 -- Date: Fri, 27 Jan 2017 16:36:00 +0000 4, 96 -- Message-ID: {1556153F-9550-4733-8FF1-A82F502036B3-at-asu.edu} 4, 96 -- References: {201701260859.v0Q8xSgh000306-at-microscopy.com} 4, 96 -- {9F74D26F-BA51-444D-8F06-AAF94D7E5915-at-asu.edu} 4, 96 -- In-Reply-To: {9F74D26F-BA51-444D-8F06-AAF94D7E5915-at-asu.edu} 4, 96 -- Accept-Language: en-US 4, 96 -- Content-Language: en-US 4, 96 -- X-MS-Has-Attach: 4, 96 -- X-MS-TNEF-Correlator: 4, 96 -- x-ms-exchange-messagesentrepresentingtype: 1 4, 96 -- x-originating-ip: [68.225.210.79] 4, 96 -- x-ms-office365-filtering-correlation-id: 63902ade-7916-4e46-7863-08d446d293d9 4, 96 -- x-microsoft-antispam: UriScan:;BCL:0;PCL:0;RULEID:(22001);SRVR:CY4PR06MB2902; 4, 96 -- x-microsoft-exchange-diagnostics: 1;CY4PR06MB2902;7:WafEdVlfJKZRpS1T2SQ6gab70fBegiu4hNvTiEwrhWDAza3wqT4M3Wcf8qZdGDxeoLqseMVP/GEdZsPl95salCJ7YvIaEdun6TesUcKFV/+fZZCNsPNjzIqN07CVoqsc6Ip4lr5AIXw7AIc2pNa2Bm69k1+F/YkXF0ObBnTGPV5WY+UJD8IU/xBmQqGqEQuVLJ94cIhr0W+03waSVoMuVQ8zXc7RfLbmhS0nNEGfiJx202yfueYxuoyxc6chfpbZRjqtm+h+YRvNb95pqp1Rg8ILo9XPGW3xHtP81l69eIhGl8A/VqDp4qlx2jeWAsIbHnO4/n6Hg4KvOEMLD4R+Ezbn/4XwMn5qassCzjov+5ep1QpX4VUYAaxXCYO6mwZvaWEwZw9B8WNHKSo2Q/rrEdR7Ni0kUIbCJe2T960IC6mbXPQszIU0/k39KDUpD27ExtnV/jXznAWdQZVQMGY9Xw== 4, 96 -- x-microsoft-antispam-prvs: {CY4PR06MB29028CD2C80996391C559555E2760-at-CY4PR06MB2902.namprd06.prod.outlook.com} 4, 96 -- x-exchange-antispam-report-test: UriScan:(162929905733931)(10436049006162)(24526216339155)(21532816269658)(201166117486090); 4, 96 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:(6040375)(601004)(2401047)(8121501046)(5005006)(3002001)(10201501046)(6041248)(20161123564025)(20161123562025)(20161123555025)(20161123560025)(6072148);SRVR:CY4PR06MB2902;BCL:0;PCL:0;RULEID:;SRVR:CY4PR06MB2902; 4, 96 -- x-forefront-prvs: 0200DDA8BE 4, 96 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10009020)(6009001)(7916002)(39450400003)(189002)(377454003)(199003)(24454002)(3846002)(102836003)(88552002)(5001770100001)(53936002)(97736004)(2521001)(189998001)(99286003)(6306002)(6116002)(6512007)(3280700002)(2906002)(81166006)(81156014)(92566002)(68736007)(122556002)(2900100001)(3660700001)(8676002)(8936002)(75432002)(82746002)(2501003)(83716003)(106116001)(105586002)(5660300001)(6506006)(2950100002)(50986999)(33656002)(54356999)(76176999)(106356001)(229853002)(86362001)(36756003)(39060400001)(107886002)(6436002)(6486002)(77096006)(25786008)(38730400001)(66066001)(575784001)(101416001)(7736002)(305945005)(2004002)(104396002)(19627235001);DIR:OUT;SFP:1101;SCL:1;SRVR:CY4PR06MB2902;H:CY4PR06MB2904.namprd06.prod.outlook.com;FPR:;SPF:None;PTR:InfoNoRecords;MX:1;A:1;LANG:en; 4, 96 -- received-spf: None (protection.outlook.com: asu.edu does not designate 4, 96 -- permitted sender hosts) 4, 96 -- spamdiagnosticoutput: 1:99 4, 96 -- spamdiagnosticmetadata: NSPM 4, 96 -- Content-Type: text/plain; charset="us-ascii" 4, 96 -- Content-ID: {65843E0843A6F94EA20BDBD0FD3404AF-at-namprd06.prod.outlook.com} 4, 96 -- MIME-Version: 1.0 4, 96 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 27 Jan 2017 16:36:00.0889 4, 96 -- (UTC) 4, 96 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted 4, 96 -- X-MS-Exchange-CrossTenant-id: 41f88ecb-ca63-404d-97dd-ab0a169fd138 4, 96 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: CY4PR06MB2902 4, 96 -- X-OriginatorOrg: asu.edu 4, 96 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:,, definitions=2017-01-27_12:,, 4, 96 -- signatures=0 4, 96 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 suspectscore=0 4, 96 -- malwarescore=0 phishscore=0 adultscore=0 bulkscore=0 classifier=spam 4, 96 -- adjust=0 reason=mlx scancount=1 engine=8.0.1-1612050000 4, 96 -- definitions=main-1701270164 4, 96 -- X-Barracuda-Connect: ppnetw4.asu.edu[10.118.52.71] 4, 96 -- X-Barracuda-Start-Time: 1485534963 4, 96 -- X-Barracuda-Encrypted: ECDHE-RSA-AES256-GCM-SHA384 4, 96 -- X-Barracuda-URL: https://149.169.2.71:443/cgi-mod/mark.cgi 4, 96 -- X-Barracuda-Scan-Msg-Size: 4986 4, 96 -- X-Virus-Scanned: by bsmtpd at asu.edu 4, 96 -- X-Barracuda-BRTS-Status: 1 4, 96 -- X-Barracuda-Spam-Score: 0.23 4, 96 -- X-Barracuda-Spam-Status: No, SCORE=0.23 using global scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=5.0 tests=MAILTO_TO_SPAM_ADDR, SH_BIG5_05413_BODY_104, THREAD_INDEX, THREAD_TOPIC 4, 96 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.36118 4, 96 -- Rule breakdown below 4, 96 -- pts rule name description 4, 96 -- ---- ---------------------- -------------------------------------------------- 4, 96 -- 0.01 THREAD_TOPIC Thread-Topic: ...(Japanese Subject)... 4, 96 -- 0.01 THREAD_INDEX thread-index: AcO7Y8iR61tzADqsRmmc5wNiFHEOig== 4, 96 -- 0.21 SH_BIG5_05413_BODY_104 BODY: Body: contain "UNSUBSCRIBE" 4, 96 -- 0.00 MAILTO_TO_SPAM_ADDR Includes a link to a likely spammer email 4, 96 -- Content-Transfer-Encoding: 8bit 4, 96 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v0RGUkGD009204 ==============================End of - Headers==============================
I have several units from ITWatchdogs. Never had any problem and they email and text me and the building managers when there is a thermal issue out of range. Interface is a standard web browser.
Scott Whittaker Lab Manager Imaging P.O. Box 37012 MRC-104 Room W150 Washington, DC 20013-7012 w 202.633.0891 whittaks-at-si.edu
SMITHSONIAN INSTITUTION NATIONAL MUSEUM OF NATURAL HISTORY
-----Original Message----- X-from: John.Mardinly-at-asu.edu [mailto:John.Mardinly-at-asu.edu] Sent: Friday, January 27, 2017 11:38 AM To: Whittaker, Scott {WHITTAKS-at-si.edu}
} I recommend a temperature sensor from Vernier.com. Their line of Go Direct sensors connect to a laptop by USB, are dirt cheap ($39 for the one I have), and come with a very nice free software package (I have never seen it crash!) for recording your measurements. I see now they have options for wireless with iOS devices. Have fun! } } } } John Mardinly, Ph.D., P.E., Retired ASU } } } } } On Jan 26, 2017, at 1:59 AM, rongchigram79-at-yahoo.com.sg wrote: } } } } } } } } } } --------------------------------------------------------------------- } } ------- The Microscopy ListServer -- CoSponsor: The Microscopy } } Society of America To Subscribe/Unsubscribe -- } } https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.co } } m_MicroscopyListserver&d=DQIBAg&c=AGbYxfJbXK67KfXyGqyv2Ejiz41FqQuZFk4 } } A-1IxfAU&r=MAuGvnWTcVQkxORgQD0QS50ZicPM3Nw-61ygSK-LNEQ&m=w7SxKtx8upRj } } cBdFpASg01gW7K2Na0pxXE9_phnExns&s=3kp26tRvY-UtqU1X55VcwWuqjnnEqOcxRc3 } } Jhq-UwKw&e= On-Line Help } } https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.co } } m_MicroscopyListserver_FAQ.html&d=DQIBAg&c=AGbYxfJbXK67KfXyGqyv2Ejiz4 } } 1FqQuZFk4A-1IxfAU&r=MAuGvnWTcVQkxORgQD0QS50ZicPM3Nw-61ygSK-LNEQ&m=w7S } } xKtx8upRjcBdFpASg01gW7K2Na0pxXE9_phnExns&s=WCAC1lAnxktP17l4ddVAVdqEv2 } } 1AGNCZxrUNpilB-XM&e= } } --------------------------------------------------------------------- } } ------- } } } } Dear All, i would like to ask for your recommendation for a temperature logger. Probably a reasonable sensitivity, acuracy and precision and is able to monitor live using the TEM pc. I am not sure if such system will interfere any performance of the TEM. If you encounter such problem, is it possible to share your experience? I do know some logger has wifi/bluetooth capability, will this interfere the TEM performances well? whiCurrently we have a temperature logger but its not live and we have to extract the data out from time to time which is very troublesome. } } } } Cheers, } } Yee Yan, Tay } } FACTS Lab } } NTU } } Singapore } } } } ==============================Original } } Headers============================== } } 2, 29 -- From rongchigram79-at-yahoo.com.sg Thu Jan 26 02:46:34 2017 2, } } 29 -- Received: from nm11-vm7.bullet.mail.sg3.yahoo.com (nm11-vm7.bullet.mail.sg3.yahoo.com [106.10.148.246]) } } 2, 29 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0Q8kWah020307 } } 2, 29 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Jan 2017 02:46:33 -0600 } } 2, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } } d=yahoo.com.sg; s=s2048; t=1485420703; } } bh=6qN0PhRcJB09HCGFUyxPVhRd9guTg7iqD/EvlCLXJnc=; } } h=Date:From:Reply-To:To:Subject:References:From:Subject; } } b=c6Tt98oAxObfHMm7NLdKppiPxSprMYMCNhxNxQTkQv+xr75cFypmnoyMLKl8r30x18L } } Ew9GLUhMnPW82J25ERJs/ajq1vyc1WlPmtfvPjCS8k4OlIJbNZ3quSlKM6Od4dO7HyIKB } } zYURwOBFQeRMlFve0yWRJawwZtmqO5UDxGPXQvEuaH6evssrmmhauYKeQTMn77OFwg3MG } } Y7e8Brz6J9WW6DmXVi2B1pLUxJLUO2mEKl3ONcYuPAwyCFVlyCQoiWKi0/iyq/tm5VT2O } } YwdXQaQysl/no1tEdu992hoP8+mgck+PRFCfnAgPVWEQi1UhQiGTDysMMfulSsOUn2WA= } } = 2, 29 -- Received: from [106.10.166.120] by } } nm11.bullet.mail.sg3.yahoo.com with NNFMP; 26 Jan 2017 08:51:43 -0000 } } 2, 29 -- Received: from [106.10.151.250] by } } tm9.bullet.mail.sg3.yahoo.com with NNFMP; 26 Jan 2017 08:51:43 -0000 } } 2, 29 -- Received: from [127.0.0.1] by omp1021.mail.sg3.yahoo.com } } with NNFMP; 26 Jan 2017 08:51:43 -0000 2, 29 -- } } X-Yahoo-Newman-Property: ymail-3 2, 29 -- X-Yahoo-Newman-Id: } } 394027.14600.bm-at-omp1021.mail.sg3.yahoo.com } } 2, 29 -- X-YMail-OSG: } } T0dUrMgVM1n6BVCBg3fxDjF5_oRmAxZT6IbMR7dm9HNOUedWZEnHbUKQk1J9kcB } } 2, 29 -- } } gvD6_0N0LbFHB20r4LPwA3jRlCbL1ChtHEJRgE2xSH2zYxjhFoYLGyMR3iQ7A2t1Jv.Wv } } s1LU.oW 2, 29 -- } } f._lJX8Zn1IsrG78tyRBPMDYndQYUl738yddERkQ4BCSJIXemHd2BVMGQGO74NOAWhwqh } } fWDck4Q 2, 29 -- } } 2ERUrDJdT0NOY8LrPgQIV7_T17Czr68KLBW2AdmAxu8y9romJ3W86r1ZZyO7m68VN4H8J } } UOXPjZ4 2, 29 -- } } fPbqB9et10u_YcROp6cw5MKXIuZHjEOeeiZJDqgDDFePjah7mBM26amiwMmaTzdMChJaJ } } Ctag4Fb 2, 29 -- } } cD87sLIE4.rkQe7brzkrpB3vZUz2kOdUcd9eR4qNjguBOmX4wdoWCeAz67M.blc0b8i9c } } zGQbt_z 2, 29 -- } } OTYSbAyRzz1HnoeHLfWA7yh_SVKjzMPItsWYOD5hjD4JONHi4QTXTWoz5f.IkXhMMePMV } } 1xDsOdI 2, 29 -- savgutwlmG_GjZL..H5GIkLrK6SsGUnDzQgW_EYMZeOc3zg-- } } 2, 29 -- Received: from jws600002.mail.sg3.yahoo.com by } } sendmailws118.mail.sg3.yahoo.com; Thu, 26 Jan 2017 08:51:42 +0000; } } 1485420702.563 2, 29 -- Date: Thu, 26 Jan 2017 08:51:42 +0000 (UTC) } } 2, 29 -- From: YY YY {rongchigram79-at-yahoo.com.sg} 2, 29 -- Reply-To: } } YY YY {rongchigram79-at-yahoo.com.sg} 2, 29 -- To: } } "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 2, 29 -- } } Message-ID: {1775741231.1160911.1485420702386-at-mail.yahoo.com} } } 2, 29 -- Subject: Temperature Logger to Recommend 2, 29 -- } } MIME-Version: 1.0 2, 29 -- Content-Type: text/plain; charset=UTF-8 2, } } 29 -- Content-Transfer-Encoding: 7bit 2, 29 -- References: } } {1775741231.1160911.1485420702386.ref-at-mail.yahoo.com} } } ==============================End of - } } Headers============================== }
==============================Original Headers============================== 4, 96 -- From John.Mardinly-at-asu.edu Fri Jan 27 10:30:47 2017 4, 96 -- Received: from bcnetw1.asu.edu (bcnetw1.asu.edu [149.169.2.71]) 4, 96 -- by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v0RGUkGD009204 4, 96 -- for {Microscopy-at-Microscopy.com} ; Fri, 27 Jan 2017 10:30:46 -0600 4, 96 -- X-ASG-Debug-ID: 1485534963-0d12c77a271103e0001-FOsErg 4, 96 -- Received: from ppnetw4.asu.edu (ppnetw4.asu.edu [10.118.52.71]) by bcnetw1.asu.edu with ESMTP id WVTnEuBXs2QOD2Jm (version=TLSv1.2 cipher=ECDHE-RSA-AES256-GCM-SHA384 bits=256 verify=NO); Fri, 27 Jan 2017 09:36:03 -0700 (MST) 4, 96 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu 4, 96 -- Received: from pps.filterd (ppnetw4.asu.edu [127.0.0.1]) 4, 96 -- by ppnetw4.asu.edu (8.16.0.17/8.16.0.17) with SMTP id v0RGZc1V021245; 4, 96 -- Fri, 27 Jan 2017 09:36:03 -0700 4, 96 -- Received: from exhubw02.asurite.ad.asu.edu (exnswvpx-10-118-52-65.inre.asu.edu [10.118.52.65]) 4, 96 -- by ppnetw4.asu.edu with ESMTP id 28893d03g7-1 4, 96 -- (version=TLSv1 cipher=ECDHE-RSA-AES256-SHA bits=256 verify=NOT); 4, 96 -- Fri, 27 Jan 2017 09:36:02 -0700 4, 96 -- Received: from NAM03-CO1-obe.outbound.protection.outlook.com (129.219.4.240) 4, 96 -- by exhubgslb.asu.edu (129.219.4.200) with Microsoft SMTP Server (TLS) id 4, 96 -- 14.3.266.1; Fri, 27 Jan 2017 09:36:02 -0700 4, 96 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 96 -- d=arizonastateu.onmicrosoft.com; s=selector1-asu-edu; 4, 96 -- h=From:Date:Subject:Message-ID:Content-Type:MIME-Version; 4, 96 -- bh=naqMZaOEeC3iExpzAGkZiww2kqIpeHTcceFQH33lSIE=; 4, 96 -- b=RoQkRKx2W8O0honOUq0kiMEoOoSFDNYyDK7BqHqvsqDO3vieIZbIin0+S6AxcCYd5ZPNfriSy0h7wSRLjDY/4juXwXMMaIqq3IDqPw0nnXOY8FkT3C2nt3GW42gp7t9ZsfJ9NRpXCSLJl1YqF2RrGdqt3w/iPOTo5a5xEYTcJxU= 4, 96 -- X-Barracuda-Effective-Source-IP: exhubw02.asurite.ad.asu.edu[129.219.4.200] 4, 96 -- X-Barracuda-Apparent-Source-IP: 129.219.4.200 4, 96 -- Received: from CY4PR06MB2904.namprd06.prod.outlook.com (10.175.118.144) by 4, 96 -- CY4PR06MB2902.namprd06.prod.outlook.com (10.175.118.142) with Microsoft SMTP 4, 96 -- Server (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_256_CBC_SHA384_P384) id 4, 96 -- 15.1.860.13; Fri, 27 Jan 2017 16:36:00 +0000 4, 96 -- Received: from CY4PR06MB2904.namprd06.prod.outlook.com ([10.175.118.144]) by 4, 96 -- CY4PR06MB2904.namprd06.prod.outlook.com ([10.175.118.144]) with mapi id 4, 96 -- 15.01.0860.023; Fri, 27 Jan 2017 16:36:00 +0000 4, 96 -- From: John Mardinly {John.Mardinly-at-asu.edu} 4, 96 -- To: "rongchigram79-at-yahoo.com.sg" {rongchigram79-at-yahoo.com.sg} , 4, 96 -- MSA Listserver 4, 96 -- {Microscopy-at-Microscopy.com} 4, 96 -- Subject: Re: [Microscopy] Temperature Logger to Recommend 4, 96 -- Thread-Topic: [Microscopy] Temperature Logger to Recommend 4, 96 -- X-ASG-Orig-Subj: Re: [Microscopy] Temperature Logger to Recommend 4, 96 -- Thread-Index: AQHSd7NBU311T3WffU6fS0JJBopyVKFMhw+AgAAAZQA= 4, 96 -- Date: Fri, 27 Jan 2017 16:36:00 +0000 4, 96 -- Message-ID: {1556153F-9550-4733-8FF1-A82F502036B3-at-asu.edu} 4, 96 -- References: {201701260859.v0Q8xSgh000306-at-microscopy.com} 4, 96 -- {9F74D26F-BA51-444D-8F06-AAF94D7E5915-at-asu.edu} 4, 96 -- In-Reply-To: {9F74D26F-BA51-444D-8F06-AAF94D7E5915-at-asu.edu} 4, 96 -- Accept-Language: en-US 4, 96 -- Content-Language: en-US 4, 96 -- X-MS-Has-Attach: 4, 96 -- X-MS-TNEF-Correlator: 4, 96 -- x-ms-exchange-messagesentrepresentingtype: 1 4, 96 -- x-originating-ip: [68.225.210.79] 4, 96 -- x-ms-office365-filtering-correlation-id: 63902ade-7916-4e46-7863-08d446d293d9 4, 96 -- x-microsoft-antispam: UriScan:;BCL:0;PCL:0;RULEID:(22001);SRVR:CY4PR06MB2902; 4, 96 -- x-microsoft-exchange-diagnostics: 1;CY4PR06MB2902;7:WafEdVlfJKZRpS1T2SQ6gab70fBegiu4hNvTiEwrhWDAza3wqT4M3Wcf8qZdGDxeoLqseMVP/GEdZsPl95salCJ7YvIaEdun6TesUcKFV/+fZZCNsPNjzIqN07CVoqsc6Ip4lr5AIXw7AIc2pNa2Bm69k1+F/YkXF0ObBnTGPV5WY+UJD8IU/xBmQqGqEQuVLJ94cIhr0W+03waSVoMuVQ8zXc7RfLbmhS0nNEGfiJx202yfueYxuoyxc6chfpbZRjqtm+h+YRvNb95pqp1Rg8ILo9XPGW3xHtP81l69eIhGl8A/VqDp4qlx2jeWAsIbHnO4/n6Hg4KvOEMLD4R+Ezbn/4XwMn5qassCzjov+5ep1QpX4VUYAaxXCYO6mwZvaWEwZw9B8WNHKSo2Q/rrEdR7Ni0kUIbCJe2T960IC6mbXPQszIU0/k39KDUpD27ExtnV/jXznAWdQZVQMGY9Xw== 4, 96 -- x-microsoft-antispam-prvs: {CY4PR06MB29028CD2C80996391C559555E2760-at-CY4PR06MB2902.namprd06.prod.outlook.com} 4, 96 -- x-exchange-antispam-report-test: UriScan:(162929905733931)(10436049006162)(24526216339155)(21532816269658)(201166117486090); 4, 96 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:(6040375)(601004)(2401047)(8121501046)(5005006)(3002001)(10201501046)(6041248)(20161123564025)(20161123562025)(20161123555025)(20161123560025)(6072148);SRVR:CY4PR06MB2902;BCL:0;PCL:0;RULEID:;SRVR:CY4PR06MB2902; 4, 96 -- x-forefront-prvs: 0200DDA8BE 4, 96 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10009020)(6009001)(7916002)(39450400003)(189002)(377454003)(199003)(24454002)(3846002)(102836003)(88552002)(5001770100001)(53936002)(97736004)(2521001)(189998001)(99286003)(6306002)(6116002)(6512007)(3280700002)(2906002)(81166006)(81156014)(92566002)(68736007)(122556002)(2900100001)(3660700001)(8676002)(8936002)(75432002)(82746002)(2501003)(83716003)(106116001)(105586002)(5660300001)(6506006)(2950100002)(50986999)(33656002)(54356999)(76176999)(106356001)(229853002)(86362001)(36756003)(39060400001)(107886002)(6436002)(6486002)(77096006)(25786008)(38730400001)(66066001)(575784001)(101416001)(7736002)(305945005)(2004002)(104396002)(19627235001);DIR:OUT;SFP:1101;SCL:1;SRVR:CY4PR06MB2902;H:CY4PR06MB2904.namprd06.prod.outlook.com;FPR:;SPF:None;PTR:InfoNoRecords;MX:1;A:1;LANG:en; 4, 96 -- received-spf: None (protection.outlook.com: asu.edu does not designate 4, 96 -- permitted sender hosts) 4, 96 -- spamdiagnosticoutput: 1:99 4, 96 -- spamdiagnosticmetadata: NSPM 4, 96 -- Content-Type: text/plain; charset="us-ascii" 4, 96 -- Content-ID: {65843E0843A6F94EA20BDBD0FD3404AF-at-namprd06.prod.outlook.com} 4, 96 -- MIME-Version: 1.0 4, 96 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 27 Jan 2017 16:36:00.0889 4, 96 -- (UTC) 4, 96 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted 4, 96 -- X-MS-Exchange-CrossTenant-id: 41f88ecb-ca63-404d-97dd-ab0a169fd138 4, 96 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: CY4PR06MB2902 4, 96 -- X-OriginatorOrg: asu.edu 4, 96 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:,, definitions=2017-01-27_12:,, 4, 96 -- signatures=0 4, 96 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 suspectscore=0 4, 96 -- malwarescore=0 phishscore=0 adultscore=0 bulkscore=0 classifier=spam 4, 96 -- adjust=0 reason=mlx scancount=1 engine=8.0.1-1612050000 4, 96 -- definitions=main-1701270164 4, 96 -- X-Barracuda-Connect: ppnetw4.asu.edu[10.118.52.71] 4, 96 -- X-Barracuda-Start-Time: 1485534963 4, 96 -- X-Barracuda-Encrypted: ECDHE-RSA-AES256-GCM-SHA384 4, 96 -- X-Barracuda-URL: https://149.169.2.71:443/cgi-mod/mark.cgi 4, 96 -- X-Barracuda-Scan-Msg-Size: 4986 4, 96 -- X-Virus-Scanned: by bsmtpd at asu.edu 4, 96 -- X-Barracuda-BRTS-Status: 1 4, 96 -- X-Barracuda-Spam-Score: 0.23 4, 96 -- X-Barracuda-Spam-Status: No, SCORE=0.23 using global scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=5.0 tests=MAILTO_TO_SPAM_ADDR, SH_BIG5_05413_BODY_104, THREAD_INDEX, THREAD_TOPIC 4, 96 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.36118 4, 96 -- Rule breakdown below 4, 96 -- pts rule name description 4, 96 -- ---- ---------------------- -------------------------------------------------- 4, 96 -- 0.01 THREAD_TOPIC Thread-Topic: ...(Japanese Subject)... 4, 96 -- 0.01 THREAD_INDEX thread-index: AcO7Y8iR61tzADqsRmmc5wNiFHEOig== 4, 96 -- 0.21 SH_BIG5_05413_BODY_104 BODY: Body: contain "UNSUBSCRIBE" 4, 96 -- 0.00 MAILTO_TO_SPAM_ADDR Includes a link to a likely spammer email 4, 96 -- Content-Transfer-Encoding: 8bit 4, 96 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v0RGUkGD009204 ==============================End of - Headers==============================
==============================Original Headers============================== 16, 28 -- From WHITTAKS-at-si.edu Fri Jan 27 10:42:14 2017 16, 28 -- Received: from si-mailout04.si.edu (si-mailout04.si.edu [160.111.103.178]) 16, 28 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0RGgDWY019647 16, 28 -- for {microscopy-at-microscopy.com} ; Fri, 27 Jan 2017 10:42:14 -0600 16, 28 -- Received: from SI-MSECHT03.US.SINET.SI.EDU (si-msecht03.us.sinet.si.edu [160.111.49.155]) 16, 28 -- by si-mailout04.si.edu (Postfix) with ESMTP id 5DE89E63AC 16, 28 -- for {microscopy-at-microscopy.com} ; Fri, 27 Jan 2017 11:47:31 -0500 (EST) 16, 28 -- Received: from SI-MSEDAG04.US.SINET.SI.EDU ([169.254.4.29]) by 16, 28 -- SI-MSECHT03.US.SINET.SI.EDU ([160.111.49.149]) with mapi id 14.03.0319.002; 16, 28 -- Fri, 27 Jan 2017 11:47:30 -0500 16, 28 -- From: "Whittaker, Scott" {WHITTAKS-at-si.edu} 16, 28 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 16, 28 -- Subject: RE: [Microscopy] Re: Temperature Logger to Recommend 16, 28 -- Thread-Topic: [Microscopy] Re: Temperature Logger to Recommend 16, 28 -- Thread-Index: AQHSeLu+b9YsafiZcEa7ntj9NbEuk6FMhzNw 16, 28 -- Date: Fri, 27 Jan 2017 16:47:29 +0000 16, 28 -- Message-ID: {5D4F637E5C599649A11693E3B6EAC6293FC5CA1E-at-si-msedag04.US.SINET.SI.EDU} 16, 28 -- References: {201701271632.v0RGWo8p010904-at-microscopy.com} 16, 28 -- In-Reply-To: {201701271632.v0RGWo8p010904-at-microscopy.com} 16, 28 -- Accept-Language: en-US 16, 28 -- Content-Language: en-US 16, 28 -- X-MS-Has-Attach: 16, 28 -- X-MS-TNEF-Correlator: 16, 28 -- x-originating-ip: [160.111.49.170] 16, 28 -- Content-Type: text/plain; charset="us-ascii" 16, 28 -- MIME-Version: 1.0 16, 28 -- Content-Transfer-Encoding: 8bit 16, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v0RGgDWY019647 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both stirling.newberry-at-gmail.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Message: Colleagues, we regret to note the passing of Sterling Newberry a long time member of the microscopy community whose passion for microscopy and education will be missed.
https://en.wikipedia.org/wiki/Sterling_Newberry
Login Host: 146.115.82.167 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
From amazon-at-boronamore.org Sun Jan 29 15:42:49 2017 Return-Path: {amazon-at-boronamore.org} Received: from bor.boronamore.org (bor.boronamore.org [192.232.196.16] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0TLgmMe000693 for {microscopylistserverarchive6-at-microscopy.com} ; Sun, 29 Jan 2017 15:42:49 -0600 DKIM-Signature: v=1; a=rsa-sha256; q=dns/txt; c=relaxed/relaxed; d=boronamore.org; s=default; h=Content-Transfer-Encoding:Content-Type: MIME-Version:Message-ID:From:Date:Subject:To:Sender:Reply-To:Cc:Content-ID: Content-Description:Resent-Date:Resent-From:Resent-Sender:Resent-To:Resent-Cc :Resent-Message-ID:In-Reply-To:References:List-Id:List-Help:List-Unsubscribe: List-Subscribe:List-Post:List-Owner:List-Archive; bh=LN7r7oyJsuymYL6J3iYMfus2P3D/RNe4SauojXPvtbQ=; b=mNYrtOzP4f1LRcYSkK7ARwYjN+ jV7aLhaCclxn/BsgycDUpCzs03CedcYjoROL5Zb1j83tkdMLpcal4atXPc4/Kwr/46OW1CbKlmUqa 3hjFecC50wVJGXItw4xmm9hnf6Ny0DTFQymDRBnIiFnlwMlSzXGVSk4sMRLUX3rIqo1YKa8Q/2FsG oJZvJwAYqs+WTlqba/dRLLSt6icohqVSW0V9vZwE3mrkzJhl2bTx+CBDULZDlI8LIZo3co1eDVhv6 Kfgrw3zq5N48j3UVrS1CeRT0kE76Pfaq1nsfOOTr01TBOBRXsHPb4BhrZkrHIhCb4JKi8fh1tuPsY skBL678g==; Received: from boss by bor.boronamore.org with local (Exim 4.87) (envelope-from {amazon-at-boronamore.org} ) id 1cXxKX-00080D-QJ for microscopylistserverarchive6-at-microscopy.com; Sun, 29 Jan 2017 15:48:13 -0600 To: microscopylistserverarchive6-at-microscopy.com
Thanks to all who responded. We have found a new home for this system. If arrangements fall through, we will select another choice from the previous inquiries.
Regards, Jim
-- Jim Passmore Principal Scientist, R&D
Sealed Air Corporation P: 864.433.2927 100 Rogers Bridge Rd., Bldg A Duncan, SC 29334
==============================Original Headers============================== 4, 34 -- From james.passmore-at-sealedair.com Mon Jan 30 07:48:09 2017 4, 34 -- Received: from mail-wm0-f42.google.com (mail-wm0-f42.google.com [74.125.82.42]) 4, 34 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0UDm92g025482 4, 34 -- for {Microscopy-at-microscopy.com} ; Mon, 30 Jan 2017 07:48:09 -0600 4, 34 -- Received: by mail-wm0-f42.google.com with SMTP id v77so48478912wmv.0 4, 34 -- for {Microscopy-at-microscopy.com} ; Mon, 30 Jan 2017 05:53:37 -0800 (PST) 4, 34 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 34 -- d=sealedair.com; s=google; 4, 34 -- h=mime-version:from:date:message-id:subject:to; 4, 34 -- bh=Qj3/kicE/70sVrrMA6dU8waubZBL4iIMs7+rWbKeS0s=; 4, 34 -- b=A78d4rAgimFjQcXCSi7tdLJZCLNLxSLLxP/9U1X2RtP+H3Mgze44ysZunEDOG4FPBu 4, 34 -- L5y2hA+rb3zdjj09nE2nt7f+9htjeRJHkUScJJIZRR00KzAQDtBGZI4i0y9+VDwB9Mu7 4, 34 -- YOV5tOVBKY4IcdiZgbrWD+IUYpZTSo5msaGcU= 4, 34 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 34 -- d=1e100.net; s=20161025; 4, 34 -- h=x-gm-message-state:mime-version:from:date:message-id:subject:to; 4, 34 -- bh=Qj3/kicE/70sVrrMA6dU8waubZBL4iIMs7+rWbKeS0s=; 4, 34 -- b=Kg4X/o0rTV5GMuwbvaj9ZcfIBgygeP/injyHafawqKdBZNbHZyk5+ANc5MhotLi2tN 4, 34 -- 7bdSlXwEqsbbWnxzSLWzrxragCJt8E4I6/hiMBxT95F7otkMmXTgWfHN1jZ1nvgVF639 4, 34 -- Kty6yHtXxMAJHSieIC/263ksbHZi/Ar9DBhAdkE3BjB5etHjvg2cfD7HVnL3CUYeanGi 4, 34 -- Jqo3LNbosrUTphYYrQyeDj7WjuIqfx8s4FF8atm0uXcH59yKP8vNyYQz+FW0NYt9QZLR 4, 34 -- zkFFy9z06ldJMLizdE5GRLO/hGeZFqkZ9asHe5YEZvCogiAgDSYAKz2ZkIgLKlEP9Ysb 4, 34 -- 8t9A== 4, 34 -- X-Gm-Message-State: AIkVDXJWheMIck3Tgrca/nCBUCYf4+v1tE4Erq52N2GfkgHMB+RAUhCmhioz9KI2EICmZyu9xGNnna5m0ovY5ap4 4, 34 -- X-Received: by 10.223.129.4 with SMTP id 4mr22600514wrm.27.1485784416628; Mon, 4, 34 -- 30 Jan 2017 05:53:36 -0800 (PST) 4, 34 -- MIME-Version: 1.0 4, 34 -- Received: by 10.194.246.161 with HTTP; Mon, 30 Jan 2017 05:53:06 -0800 (PST) 4, 34 -- From: Jim Passmore {james.passmore-at-sealedair.com} 4, 34 -- Date: Mon, 30 Jan 2017 08:53:06 -0500 4, 34 -- Message-ID: {CAGV-iYavoM+mJi-4CFWv_TRvfh0Taypq+RXnq_HCoq+50+Z=Sg-at-mail.gmail.com} 4, 34 -- Subject: XPS/ESCA system has new home 4, 34 -- To: microscopy {Microscopy-at-microscopy.com} 4, 34 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
From smithdiana157-at-gmail.com Mon Jan 30 11:59:01 2017 Return-Path: {smithdiana157-at-gmail.com} Received: from gmail.com (mail.dongnamdc.com [222.97.39.100] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v0UHwxZ0019571 for {microscopylistserverarchive6-at-microscopy.com} ; Mon, 30 Jan 2017 11:59:00 -0600 Message-ID: {AB757720.37CE6E18-at-gmail.com}
X-from: leandro.lemgrubersoares-at-glasgow.ac.uk
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both leandro.lemgrubersoares-at-glasgow.ac.uk as well as the Microscopy Listserver ---------------------------------------------------------------------------
Message: Dear All, does anyone know if there is a membrane permeable dye or probe that recognises HA-tag protein in live imaging?
Thanks!
Login Host: 130.209.127.194 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
} Begin forwarded message: } } From: Nancy Gray {ngray-at-grc.org} } Subject: Re: [3dem] GRC Locations } Date: January 31, 2017 at 19:19:24 GMT+1 } To: "Smith, Phillip R." {smithp01-at-nyumc.org} , Marin van Heel {marin.vanheel-at-googlemail.com} } Cc: Henning Stahlberg {henning.stahlberg-at-unibas.ch} , Microscopy {Microscopy-at-microscopy.com} , 3dem {3dem-at-ncmir.ucsd.edu} , "CCPEM-at-JISCMAIL.AC.UK" {CCPEM-at-JISCMAIL.AC.UK} } } Folks, } Could you kindly forward my response to your network since my attempt was rejected since I am not authorized. I am getting many emails on the issue and would like all to know GRC is formulating a response. Many thanks, } Nancy } } Nancy Ryan Gray, PhD } Gordon Research Conferences, President and Chief Executive Officer } 512 Liberty Lane } West Kingston, RI 02892 } 401-360-1521 } www.grc.org } } } } On Jan 31, 2017, at 11:33 AM, Nancy Gray {ngray-at-grc.org} wrote: } } } } Dear Henning, Ulrike, Stefan, Marin, Ross and members of the 3dem mailing list, } } } } Thank you for your input, it is indeed a difficult situation. I have scheduled a conference call with the GRC Board of Trustees Executive Committee and GRC Legal Counsel to address these concerns and will get back to you shortly. } } Thanks for your support, patience and understanding. } } Nancy } } } } } } } } Nancy Ryan Gray, PhD, President and Chief Executive Officer } } Gordon Research Conferences } } 512 Liberty Lane, West Kingston, RI 02892-1502 } } Phone: 401-360-1521 l Fax: 401-783-7644 } } Email: ngray-at-grc.org l Web: http://www.grc.org
[]
} } } From: Henning Stahlberg {henning.stahlberg-at-unibas.ch} } } } Subject: [3dem] GRC Locations } } } Date: January 30, 2017 at 21:08:10 GMT+1 } } } To: "Nancy R. Gray" {NGray-at-grc.org} } } } Cc: Microscopy {Microscopy-at-microscopy.com} , 3dem {3dem-at-ncmir.ucsd.edu} } } } } } } Dear Nancy, } } } } } } Science is open and international. The US is currently not, unfortunately. } } } Until that situation is resolved, may I ask you to consider moving the upcoming GRC conferences to openly accessible locations, such as international conference sites as in Cancun in Mexico or in other countries. } } } This would allow fair and equal access of scientist from all countries and religions to your conferences, and prevent a boycott movement from forming. } } } Your conferences in Europe and Asia are a great start. } } } } } } With best wishes, } } } } } } Henning Stahlberg. } } } } } } } } } Henning Stahlberg, PhD } } } Prof. for Structural Biology, C-CINA, Biozentrum, University Basel } } } Mattenstrasse 26 | D-BSSE | WRO-1058 | CH-4058 Basel | Switzerland } } } http://c-cina.org | Tel. +41-61-387 32 62 } } }
==============================Original Headers============================== 6, 35 -- From henning.stahlberg-at-unibas.ch Tue Jan 31 14:38:40 2017 6, 35 -- Received: from mx2-priv.urz.unibas.ch (mx2-priv.urz.unibas.ch [131.152.226.165]) 6, 35 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0VKcd6O014824 6, 35 -- for {Microscopy-at-microscopy.com} ; Tue, 31 Jan 2017 14:38:40 -0600 6, 35 -- Received: from localhost (localhost [127.0.0.1]) 6, 35 -- by mx2-priv.urz.unibas.ch (Postfix) with ESMTP id C28DE22019F; 6, 35 -- Tue, 31 Jan 2017 21:44:11 +0100 (CET) 6, 35 -- X-Virus-Scanned: amavisd-new at unibas.ch 6, 35 -- Received: from mx2-priv.urz.unibas.ch ([131.152.226.165]) 6, 35 -- by localhost (mx2-mgnt.urz.unibas.ch [127.0.0.1]) (amavisd-new, port 10024) 6, 35 -- with LMTP id JEJbqur-zeLN; Tue, 31 Jan 2017 21:44:11 +0100 (CET) 6, 35 -- Received: from URZ-HT-CAS-4.urz.unibas.ch (urz-ht-cas-4.urz.unibas.ch [131.152.8.134]) 6, 35 -- by mx2-priv.urz.unibas.ch (Postfix) with ESMTPS id B1686220188; 6, 35 -- Tue, 31 Jan 2017 21:44:11 +0100 (CET) 6, 35 -- Received: from URZ-MBX-1.urz.unibas.ch ([131.152.8.141]) by 6, 35 -- URZ-HT-CAS-4.urz.unibas.ch ([fe80::1c8f:5331:992b:ec37%15]) with mapi id 6, 35 -- 14.03.0319.002; Tue, 31 Jan 2017 21:44:11 +0100 6, 35 -- From: Henning Stahlberg {henning.stahlberg-at-unibas.ch} 6, 35 -- To: 3dem {3dem-at-ncmir.ucsd.edu} , Microscopy {Microscopy-at-microscopy.com} 6, 35 -- Subject: Fwd: [3dem] GRC Locations 6, 35 -- Thread-Topic: [3dem] GRC Locations 6, 35 -- Thread-Index: AQHSezSUjSU5xLihGUqfyWAj4PWHXA== 6, 35 -- Date: Tue, 31 Jan 2017 20:44:11 +0000 6, 35 -- Message-ID: {715A7637-36FD-489D-A5C1-D2C10BACA42D-at-unibas.ch} 6, 35 -- References: {EBA6B95B-6ED4-4414-A50C-CB35C4BFFE48-at-grc.org} 6, 35 -- Accept-Language: en-US, de-CH 6, 35 -- Content-Language: en-US 6, 35 -- X-MS-Has-Attach: 6, 35 -- X-MS-TNEF-Correlator: 6, 35 -- x-originating-ip: [131.152.226.242] 6, 35 -- Content-Type: text/plain; charset="Windows-1252" 6, 35 -- Content-ID: {93EE1C68CEF34441BCD0B44F6BFDC3D4-at-exch.unibas.ch} 6, 35 -- MIME-Version: 1.0 6, 35 -- Content-Transfer-Encoding: 8bit 6, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v0VKcd6O014824 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both erosen-at-mednet.ucla.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: erosen-at-mednet.ucla.edu Name: Eric Rosen Organization: UCLA Medical Center Title-Subject: [Filtered] Histo / EM position open Message: Hi all,
We have a 50% Histo/50% EM position open at UCLA hospital.
See job description here: http://www.uclahealthcareers.org/all-jobs/Histotechnologist-II/H88333 Login Host: 149.142.103.151 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
One day too late, but anyway happy new year to all, as it is my first post of the year !
I'm looking if someone could have soon written a bash script (or else working on linux system) to convert in batch mode EDAX spectra from the .spc file format to .emsa/msa. The "Export" function of the TEAM software has only the .spc format as output, and the "Send_To_Folder" function is far too time consuming for saving a project part with tenth of spectra, in particular as it doesn't keep the original names of the data.
I tried Spectrum Viewer but I didn't bring it to work on linux (via Wine, but I'm very binary with Wine : it works or doesn't work... I've no time to learn to configure it !).
Fortunately, DTSA-II reads the .spc format and can save in emsa, what allows me to work. But DTSA has not the goal to do batch format conversions !
Thanks to all
Jacques
J. Faerber IPCMS-DSI Institut de Physique et Chimie des Matériaux de Strasbourg Département Surfaces et Interfaces 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
The open-source HyperSpy software (www.hyperspy.org) can do what you are looking for. EDAX file reading (both .spd and .spc) has been added in the latest development builds (and should be included in the next minor release -- v1.2). If you cannot wait for that, setting up a copy of the development version isn't too difficult if you have some proficiency with Python and git (http://hyperspy.org/hyperspy-doc/current/user_guide/install.html#development-version).
Good luck!
- Josh
NB: Any mention of commercial products or software is for information only; it does not imply recommendation or endorsement by NIST.
-- Joshua Taillon, Ph.D. Materials Measurement Science Division National Institute of Standards and Technology
-----Original Message----- X-from: jacques.faerber-at-ipcms.unistra.fr [mailto:jacques.faerber-at-ipcms.unistra.fr] Sent: Wednesday, February 1, 2017 4:42 AM To: Taillon, Joshua A. (Fed) {joshua.taillon-at-nist.gov}
Hi all
One day too late, but anyway happy new year to all, as it is my first post of the year !
I'm looking if someone could have soon written a bash script (or else working on linux system) to convert in batch mode EDAX spectra from the .spc file format to .emsa/msa. The "Export" function of the TEAM software has only the .spc format as output, and the "Send_To_Folder" function is far too time consuming for saving a project part with tenth of spectra, in particular as it doesn't keep the original names of the data.
I tried Spectrum Viewer but I didn't bring it to work on linux (via Wine, but I'm very binary with Wine : it works or doesn't work... I've no time to learn to configure it !).
Fortunately, DTSA-II reads the .spc format and can save in emsa, what allows me to work. But DTSA has not the goal to do batch format conversions !
Thanks to all
Jacques
J. Faerber IPCMS-DSI Institut de Physique et Chimie des Matériaux de Strasbourg Département Surfaces et Interfaces 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
Unless someone have already written and would share the script you need for converting .spc to .emsa, it should be possible to automate sequential opening of files within a folder by DTSA-II, saving data in another format, and repeating the operation until all files are processed with one of GUI scripting tools. I am using WinBatch (US$100 from http://www.windowware.com/, no connection just a happy user) for file processing purposes, although in different then yours application. There is also AutoIt which is free from www.autoitscript.com, but I did not try it. Not aware of similar GUI scripting tools for Linux, but would be surprised if they didn't exist as well.
Valery Ray www.linkedin.com/in/valeryray ============================== PBS&T, MEO Engineering Company 290 Broadway, Suite 298 Methuen, MA 01844, USA Phone: +1-978-305-0479 - leave a message Mobie: +1-978-305-0479 - leave a message E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 2/1/2017 4:20 AM, jacques.faerber-at-ipcms.unistra.fr wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi all } } One day too late, but anyway happy new year to all, as it is my first } post of the year ! } } I'm looking if someone could have soon written a bash script (or else } working on linux system) to convert in batch mode EDAX spectra from the } .spc file format to .emsa/msa. } The "Export" function of the TEAM software has only the .spc format as } output, and the "Send_To_Folder" function is far too time consuming for } saving a project part with tenth of spectra, in particular as it doesn't } keep the original names of the data. } } I tried Spectrum Viewer but I didn't bring it to work on linux (via } Wine, but I'm very binary with Wine : it works or doesn't work... I've } no time to learn to configure it !). } } Fortunately, DTSA-II reads the .spc format and can save in emsa, what } allows me to work. But DTSA has not the goal to do batch format } conversions ! } } Thanks to all } } Jacques } } J. Faerber } IPCMS-DSI } Institut de Physique et Chimie des Matériaux de Strasbourg } Département Surfaces et Interfaces } 23, rue de Loess ; BP43 } 67034 Strasbourg CEDEX 2 } France } } Tel 00 33(0)3 88 10 71 01 } Fax 00 33(0)3 88 10 72 48 } E-mail : Jacques.Faerber-at-ipcms.unistra.fr } } } ==============================Original Headers============================== } 10, 26 -- From jacques.faerber-at-ipcms.unistra.fr Wed Feb 1 03:19:35 2017 } 10, 26 -- Received: from webmail.ipcms.fr (webmail.ipcms.fr [130.79.210.2]) } 10, 26 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v119JY3g019013 } 10, 26 -- for {microscopy-at-microscopy.com} ; Wed, 1 Feb 2017 03:19:35 -0600 } 10, 26 -- Received: from localhost (localhost [127.0.0.1]) } 10, 26 -- by webmail.ipcms.fr (Postfix) with ESMTP id A48AFE718E } 10, 26 -- for {microscopy-at-microscopy.com} ; Wed, 1 Feb 2017 10:25:08 +0100 (CET) } 10, 26 -- X-Virus-Scanned: Debian amavisd-new at webmail.ipcms.fr } 10, 26 -- Received: from webmail.ipcms.fr ([127.0.0.1]) } 10, 26 -- by localhost (webmail.ipcms.fr [127.0.0.1]) (amavisd-new, port 10024) } 10, 26 -- with ESMTP id SZ6XiuzgMIwg for {microscopy-at-microscopy.com} ; } 10, 26 -- Wed, 1 Feb 2017 10:25:07 +0100 (CET) } 10, 26 -- Received: from wing.ipcms1.ipcms.unistra.fr (wing.u-strasbg.fr [130.79.153.228]) } 10, 26 -- (Authenticated sender: jfaerber-at-ipcms.unistra.fr) } 10, 26 -- by webmail.ipcms.fr (Postfix) with ESMTPSA id 99B62E70F5 } 10, 26 -- for {microscopy-at-microscopy.com} ; Wed, 1 Feb 2017 10:25:07 +0100 (CET) } 10, 26 -- From: jacques faerber {jacques.faerber-at-ipcms.unistra.fr} } 10, 26 -- Subject: EDAX .spc to .emsa format batch conversion } 10, 26 -- To: microscopy-at-microscopy.com } 10, 26 -- Message-ID: {a7b5d9c4-ce93-8c03-ff5c-48ed6903ec72-at-ipcms.unistra.fr} } 10, 26 -- Date: Wed, 1 Feb 2017 10:25:07 +0100 } 10, 26 -- User-Agent: Mozilla/5.0 (X11; Linux x86_64; rv:45.0) Gecko/20100101 } 10, 26 -- Thunderbird/45.5.1 } 10, 26 -- MIME-Version: 1.0 } 10, 26 -- Content-Type: text/plain; charset=utf-8; format=flowed } 10, 26 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers============================== }
==============================Original Headers============================== 4, 37 -- From vray-at-partbeamsystech.com Wed Feb 1 10:28:11 2017 4, 37 -- Received: from nm31.bullet.mail.gq1.yahoo.com (nm31.bullet.mail.gq1.yahoo.com [98.136.217.14]) 4, 37 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v11GSBXn012214 4, 37 -- for {microscopy-at-microscopy.com} ; Wed, 1 Feb 2017 10:28:11 -0600 4, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1485966826; bh=Nq3d7mdXxW5hEuoMWPME5ggCOt4MTExdXYfZW8KzXvM=; h=Subject:To:References:From:Cc:Date:In-Reply-To:From:Subject; b=OUJMbdQ34+2x91oNmSiKyRkYyRuLgujCYaPkZMTZR4qzCbxo8GSgMno7elxw4Fu5QYAYAL8187krgMZJeFXgJF6zin3WpTi+xH0ef75/m4GuvTCns54IYpemjPU93z49V5cZGe2i50rszon0DDaFX+NYxOe/xEYUiq+3gcTCHcVqh7f1wAV2QdvYTxorZqBB42yHB7nUCKW2VhUZH4jWhp608in3HiyjhFgJkc9PXzjSuvTVAiRrhzaJwP5qpkwyY6Fw0gpIEREpd0C/zKAYVHTl2CNxWs8xNrfhpmTD2rQR7iGJ41iQSZUriotkyBfw65JrAivu7oS9yUZKigXfQw== 4, 37 -- Received: from [127.0.0.1] by nm31.bullet.mail.gq1.yahoo.com with NNFMP; 01 Feb 2017 16:33:46 -0000 4, 37 -- Received: from [98.137.12.62] by nm31.bullet.mail.gq1.yahoo.com with NNFMP; 01 Feb 2017 16:31:00 -0000 4, 37 -- Received: from [98.139.170.182] by tm7.bullet.mail.gq1.yahoo.com with NNFMP; 01 Feb 2017 16:31:00 -0000 4, 37 -- Received: from [68.142.230.76] by tm25.bullet.mail.bf1.yahoo.com with NNFMP; 01 Feb 2017 16:31:00 -0000 4, 37 -- Received: from [127.0.0.1] by smtp233.mail.bf1.yahoo.com with NNFMP; 01 Feb 2017 16:31:00 -0000 4, 37 -- X-Yahoo-Newman-Id: 16718.77480.bm-at-smtp233.mail.bf1.yahoo.com 4, 37 -- X-Yahoo-Newman-Property: ymail-4 4, 37 -- X-YMail-OSG: ooZZ4MMVM1lfEWO_ftr1.OYqw12hW63xZJm86rq8uS1NVZH 4, 37 -- rTvHFCcBqe4eW0HAoG1n0BLC4miV02Le7i2fCfAmn7mHynCQaOXw6Q3Sq836 4, 37 -- sL__wB7kVZ8t5YiFnfnIBj0frk9ToMiC9Y1ZWtAaTdscattcfqhbpheU7uUP 4, 37 -- D6ikZYDZAxpHiBsHp2edv8bYL_h1HA1U5V9CG1oWfeaWHjj7UYWzbk0oab0s 4, 37 -- v1eyVpu..74LK6bu0mhrmj0LA0sX.BCGT70XlYwfeZ.2cyHe6nfx7VfMSzee 4, 37 -- 4z9jXmG5x_kb1pjwrofPeGL2CqGmfkzJAoQX6q69KbLidClhLwHTNuM1JWEw 4, 37 -- .H0Ayf76w6S.VljFFU6Cy07XVcrlZJBMTlUH7b8B6SykNyZ7uaxDOvhh1RKr 4, 37 -- UBdtxtDxLDd5mCxIAPLynn1c4S_dalpbXlqk_eWBwYiaAysbBbm9nBHPATeq 4, 37 -- aNY4JBk5s2ojP_pcdwSVFWH_MzYNrXK4mGM4_OEhDZnan0DRUR88GI6nM_N4 4, 37 -- RHrgrJ9yJEo5O55ObmArN9tWKyOIqDbrzsGW7If88tN3UbKcq6F32OKW2P4i 4, 37 -- NotAaZhrOVRpeUdPZobSq 4, 37 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 4, 37 -- Subject: Re: [Microscopy] EDAX .spc to .emsa format batch conversion 4, 37 -- To: jacques.faerber-at-ipcms.unistra.fr 4, 37 -- References: {201702010920.v119KpQx020159-at-microscopy.com} 4, 37 -- From: Valery Ray {vray-at-partbeamsystech.com} 4, 37 -- Cc: microscopy-at-microscopy.com 4, 37 -- Message-ID: {e64844c1-23be-5627-649d-03bae2544509-at-partbeamsystech.com} 4, 37 -- Date: Wed, 1 Feb 2017 11:28:22 -0500 4, 37 -- User-Agent: Mozilla/5.0 (Windows NT 10.0; WOW64; rv:45.0) Gecko/20100101 4, 37 -- Thunderbird/45.7.0 4, 37 -- MIME-Version: 1.0 4, 37 -- In-Reply-To: {201702010920.v119KpQx020159-at-microscopy.com} 4, 37 -- Content-Type: text/plain; charset=windows-1252; format=flowed 4, 37 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
Jacques Faerber asked about a script for the conversion of EDAX .spc to msa format.
DTSA-II has a nice scripting language (jython) that permits this. One can also apply a DTSA calibrated detector instance to the spectrum. I sent Jacques amd earlier version of this this morning. I want to make certain that the formatting stays correct, so I created a public gist on GitHub. You can obtain the script at:
Hello Everyone, Our 1 meg Gatan Camera (model 780 Duel vision) on our TEM is dying and we want to give it a transplant. I'm looking for a cooling fan.
It's the 780.PL6FA model, if that helps. It's from the turn of the century and if you have a one that's broken and the fan works or you have a spare fan or know of a replacement fan, I sure would like to hear from you.
Thanks in advance........ Frank Karl ARDL 330-794-6600
This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc.
==============================Original Headers============================== 6, 28 -- From frank_karl-at-ardl.com Wed Feb 1 14:04:51 2017 6, 28 -- Received: from cal1-mh745b.smtproutes.com (cal1-mh745b.smtproutes.com [208.70.89.155]) 6, 28 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v11K4oeI031609 6, 28 -- for {microscopy-at-microscopy.com} ; Wed, 1 Feb 2017 14:04:51 -0600 6, 28 -- X-Katharion-ID: 1485979808.49884.cal1-mh745 6, 28 -- Received: from mail.ardl.com ([74.207.215.218]) by 6, 28 -- cal1-mh745.smtproutes.com [(192.69.16.67)] with ESMTP via TCP 6, 28 -- (TLSv1/TLS_RSA_WITH_AES_128_CBC_SHA); 01 Feb 2017 20:10:08 +0000 6, 28 -- Received: from exchange2k7.ad.ardl.com ([fe80::d57a:b38c:8f17:9f73]) by 6, 28 -- exchange2k7.ad.ardl.com ([fe80::d57a:b38c:8f17:9f73%15]) with mapi; Wed, 1 6, 28 -- Feb 2017 15:10:07 -0500 6, 28 -- From: Frank Karl {frank_karl-at-ardl.com} 6, 28 -- To: "Microscopy Listserver (microscopy-at-microscopy.com)" 6, 28 -- {microscopy-at-microscopy.com} 6, 28 -- Date: Wed, 1 Feb 2017 15:10:06 -0500 6, 28 -- Subject: Our TEM needs a cool breeze..... 6, 28 -- Thread-Topic: Our TEM needs a cool breeze..... 6, 28 -- Thread-Index: AdJ8xy5dvYNL9ahiQhCUYpRgYVG00w== 6, 28 -- Message-ID: {0445DA8FFC235F4CBDAF9BACCF79E1212CC3813F86-at-exchange2k7.ad.ardl.com} 6, 28 -- Accept-Language: en-US 6, 28 -- Content-Language: en-US 6, 28 -- X-MS-Has-Attach: 6, 28 -- X-MS-TNEF-Correlator: 6, 28 -- acceptlanguage: en-US 6, 28 -- Content-Type: text/plain; charset="us-ascii" 6, 28 -- MIME-Version: 1.0 6, 28 -- Content-Transfer-Encoding: 8bit 6, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v11K4oeI031609 ==============================End of - Headers==============================
I am an accomplished Electron Microscopy Technician, supervisor, and research specialist with 25 years experience working in both clinical and research environments looking for a position where my acquired skills can be used for professional advancement.
Posting of "looking for a position" emails has never been permitted. Please do not do this.
There are job boards, one specifically hosted and run by the Microscopy Society of America http://jobs.microscopy.org/ that fulfill this role.
Nestor Your Friendly Neighborhood SysCop
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both nlamine6-at-hotmail.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: nlamine6-at-hotmail.com Name: naitbouda
Organization: cdta
Title-Subject: [Filtered] strip
Message: Hello
hello the size of jeol 5600LV and JEOL 6360LV IS THE SAME the Jeol strip apertures 3 holes are/ 20micron -30um-100um
you can use the size of Jeol strip aperture 9.0x2.35x0.10mm (platinium-Iridium)
Part:67001-10-20-100
best regards Login Host: 41.110.187.86 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
Thank you for your efforts to disseminate your communications to a larger audience. This is an important issue from many perspectives and I imagine most subscribers to this list will be very interested in how the GRC responds. I would suggest that scientific societies all ought to have a measured and well thought out position on the current political situation, and that those positions be made abundantly clear world-wide. From my personal perspective, I feel boycotts are unhelpful in resolving larger issues, especially when the boycotting group has little to no true leverage other than to shoot itself in the foot.
Robert J. Palmer Jr., PhD National Institute of Dental and Craniofacial Research National Institutes of Health Building 30, Room 331 Bethesda MD 20892 301-594-0025
On 1/31/17, 4:00 PM, "henning.stahlberg-at-unibas.ch" {henning.stahlberg-at-unibas.ch} wrote:
} } } } -------------------------------------------------------------------------- } -- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both lon.nelson-at-leica-microsystems.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: lon.nelson-at-leica-microsystems.com Name: Lon Nelson
Message: Leica Microsystems is currently seeking a commissioned Sales Agent or Dealer in the Southeast USA for our Electron Microscopy Sample Preparation solutions.
https://tinyurl.com/leicaEMprep
Interested parties should contact Lon Nelson, Director of Sales, at lon.nelson-at-leica-microsystems.com.
Best regards,
Lon Nelson Director of Sales Microscopy lon.nelson-at-leica-microsystems.com M +1 224-628-2467 | F +1 847-607-3160 Leica Microsystems, Inc. 1700 Leider Ln | Buffalo Grove, IL 60089 (USA)
Login Host: 76.231.70.70 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
The deadline for paper submission for M&M 2017 is less than two weeks away and as you are making plans to attend the St. Louis meetings (Aug. 6-10), please don't forget about MSA's student bursary program. Its purpose is to encourage students to attend the meetings by helping to defray some of the costs while giving them an opportunity to meet and interact with the established microscopy community.
The student bursaries will be paid $10 an hour to work for ~20 hours during the meeting or pre-meeting events. The jobs involve such things as providing support in the different symposia, staffing the volunteer office; newsletter distribution, and helping with vendor tutorial sign-up.
Once the program has been finalized, each registered bursary will be contacted and given the chance to choose the times and activities where they would like to help. There is an added bonus of $10 cash for each morning and/or afternoon session worked to assist with meals and a meeting shirt.
If anyone would like to participate in the bursary program, please check the box "I wish to apply for a student bursary" in section 2 of the registration form. Applicants for the bursaries must be members of MSA or MAS, enrolled as students at a recognized educational institution, and pay their registration fee. Bursary space is limited, so sign-up early.
If anyone has any questions about the bursary program, or would like to participate, please contact Amanda (alawrence-at-i2at.msstate.edu).
Also don't forget about the opportunities for meeting awards!
Amanda Lawrence Institute for Imaging and Analytical Technologies Mississippi State University 662-325-7998 alawrence-at-i2at.msstate.edu
==============================Original Headers============================== 9, 32 -- From ALawrence-at-i2at.msstate.edu Thu Feb 2 11:28:22 2017 9, 32 -- Received: from catalpa.its.msstate.edu (catalpa.its.msstate.edu [130.18.2.119]) 9, 32 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v12HSMfZ006970 9, 32 -- for {Microscopy-at-microscopy.com} ; Thu, 2 Feb 2017 11:28:22 -0600 9, 32 -- Received: from mail01.ad.msstate.edu (mail01.ad.msstate.edu [130.18.230.60]) 9, 32 -- by catalpa.its.msstate.edu (8.13.8/8.13.8) with ESMTP id v12HY16S019256 9, 32 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=FAIL) 9, 32 -- for {Microscopy-at-microscopy.com} ; Thu, 2 Feb 2017 11:34:01 -0600 9, 32 -- X-Sender: {} 9, 32 -- Received: from MAIL02.ad.msstate.edu (2620:0:1a30:1230::61) by 9, 32 -- mail01.ad.msstate.edu (2620:0:1a30:1230::60) with Microsoft SMTP Server (TLS) 9, 32 -- id 15.0.1236.3; Thu, 2 Feb 2017 11:34:01 -0600 9, 32 -- Received: from MAIL02.ad.msstate.edu ([fe80::7846:3039:9492:24b0]) by 9, 32 -- mail02.ad.msstate.edu ([fe80::7846:3039:9492:24b0%13]) with mapi id 9, 32 -- 15.00.1236.000; Thu, 2 Feb 2017 11:34:01 -0600 9, 32 -- From: "Lawrence, Amanda" {ALawrence-at-i2at.msstate.edu} 9, 32 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 9, 32 -- Subject: M&M 2017 meeting - Student Bursaries 9, 32 -- Thread-Topic: M&M 2017 meeting - Student Bursaries 9, 32 -- Thread-Index: AdJ9eZ4IMkJzdOivRvW3xlKpqOpYMQ== 9, 32 -- Date: Thu, 2 Feb 2017 17:34:00 +0000 9, 32 -- Message-ID: {e24808588a88462983e02454d3ce081e-at-mail02.ad.msstate.edu} 9, 32 -- Accept-Language: en-US 9, 32 -- Content-Language: en-US 9, 32 -- X-MS-Has-Attach: 9, 32 -- X-MS-TNEF-Correlator: 9, 32 -- x-ms-exchange-transport-fromentityheader: Hosted 9, 32 -- x-originating-ip: [130.18.230.93] 9, 32 -- Content-Type: text/plain; charset="us-ascii" 9, 32 -- MIME-Version: 1.0 9, 32 -- Content-Transfer-Encoding: 8bit 9, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v12HSMfZ006970 ==============================End of - Headers==============================
X-from: owen.neill-at-wsu.edu To: Zaluzec-at-microscopy.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both owen.neill-at-wsu.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: owen.neill-at-wsu.edu Name: Owen Neill
Organization: Peter Hooper GeoAnalytical Lab, Washington State University
Title-Subject: [Filtered] M&M2017 - Symposium A09
Message: Dear colleagues, Apologies for the cross-postings, but I hope 2017 is off to a good start for everyone. Paper submission for the Microscopy and Microanalysis 2017 Annual Meeting (St. Louis, MO, 6-10 August, 2017) is now open, and will close on 15 February. My co-conveners and I would like to draw your attention to: Symposium A09, Standards, Reference Materials, and Their Applications in Quantitative Microanalysis. We are looking for submissions dealing with the synthesis, evaluation, and need for new reference materials; evaluation, distribution, and maintenance of existing reference materials; the use of standards in quantitative microanalysis; and the application of quantitative microanalytical techniques to solving analytical problems. A full description of this session is included below. Papers may be submitted via the M&M2017 website: http://www.microscopy.org/mandm/2017/ We are also pleased to announce the invited speakers for this symposium: John Hanchar, Department of Earth Sciences, Memorial University, Newfoundland William Nachlas, Department of Earth Sciences, Syracuse University Timothy Rose, Department of Mineral Sciences, Smithsonian Institution Stephen Wilson, Reference Materials Program, United States Geological Survey We are very excited to hear from these experts in microanalytical standards and quantitative microanalysis, and we look forward to hearing about your work as well. See you in St. Louis! The organizers of Symposium A09: Julien Allaz, University of Colorado Boulder Anette von der Handt, University of Minnesota Twin Cities Owen Neill, Washington State University Symposium Description: Standards and reference materials are essential for obtaining accurate quantitative compositional data from X-ray microanalysis by EPMA or SEM (WDS/EDS), as well as from other microanalytical techniques (LA-ICP-MS, SIMS, µ-XRF, FTIR, Raman spectroscopy, etc.). These materials must be rigorously evaluated for their reference compositions and homogeneity, must be widely available to the analytical community, and must be properly maintained to avoid contamination or deterioration. We welcome contributions on the synthesis, evaluation, distribution, and maintenance of standards and reference materials, as well as their appropriate use in microanalysis. We further encourage submissions on standard-based applications of quantitative microanalysis, or on the development of new quantitative microanalytical protocols.
Topics of interest include: The use of standards and reference materials in quantitative microanalysis, and the needs of the analytical community for improving such materials. Synthesis, evaluation, distribution, and maintenance of standards and reference materials. Development of new protocols for microanalytical techniques. Applications of standard-based techniques to solving microanalytical problems.
Login Host: 134.121.47.10 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- ============================================= Do no rely to this messaage it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jhyun-at-gatan.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: jhyun-at-gatan.com Name: John Hyun
Organization: Gatan
Title-Subject: [Filtered] dvanced EELS and EFTEM Training School - France 2017
Message: The Advanced EELS and EFTEM Training School hosted by EDF R&D is an intensive 4-day training school that incorporates lectures, computer laboratories, and microscope practical classes to provide participants with a comprehensive, hands-on training on advanced electron energy loss spectroscopy (EELS) and energy-filtered transmission electron microscopy (EFTEM) topics.
The practical sessions on the microscope will be given at the Materials Ageing Institute. This course focuses on the advanced practice of EELS based imaging and analysis in the (S)TEM microscope on advanced based EELS techniques.
A good experience with electron microscopy and EELS is highly recommended. By the end of the course, participants can expect to get the most out of their EELS spectrometer system and know how to best optimize the experimental conditions in order to capture the maximum amount of information out of the TEM samples using EELS.
Online information and registration: http://www.gatan.com/company/events/advanced-eels-and-eftem-training-school-france-2017
Login Host: 50.58.128.11 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- ============================================= Do no rely to this messaage it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
From anittiff04-at-gmail.com Thu Feb 2 16:11:29 2017 Return-Path: {anittiff04-at-gmail.com} Received: from gmail.com ([218.232.94.109]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v12MBOUM002461 for {microscopylistserverarchive6-at-microscopy.com} ; Thu, 2 Feb 2017 16:11:28 -0600 Message-ID: {7CCED538.2E612E5E-at-gmail.com}
X-from: jwsandinod-at-unal.edu.co
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jwsandinod-at-unal.edu.co as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: jwsandinod-at-unal.edu.co Name: John W. Sandino
Organization: Universidad Nacional de Colombia
Title-Subject: [Filtered] Need Gatan 794 Camera Control
Message: Two years ago we received an old 794 retractable camera Gatan as donation from the Tribenberg Lab (closed). But two days ago the camera controller of this camera does not work any more. Today the gatan people confirm the news and told as that the solution would buy a new camera. However we don't have budget for that.
We ask for some who could have a camara contoller that coud give us for free. I know that it is no the best petition but for the moment is the unique solution to work with our tecnai 20. one of the best microscopes in our country Colombia.
Thanks Login Host: 168.176.239.31 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mreedman-at-rms.org.uk as well as the Microscopy Listserver ---------------------------------------------------------------------------
Message: mmc2017 and EMAG 2017 Abstract Submission Deadline - 17 February ---------------------------------------------------
The Microscience Microscopy Congress is returning to Manchester from 3 - 6 July 2017 and now is the time to join us at this great event by submitting an abstract!
www.mmc-series.org.uk/abstracts There is only 2 weeks left to submit an oral abstract as submission closes on Friday 17 February 2017.
The Call for Papers covers a wide variety of topics and techniques as you can see below:
Life Sciences Sessions - Bio Applications: Investigating Biological Structure using Electron Tomography - Imaging in Flow Cytometry - Imaging protein dynamics in living cells - Investigating Biological Structure using Electron Tomography - Bio Applications: Imaging Cells in 3D - matrix, tissue, in vivo - Bio EM Sample Preparation and Analysis - Bio Applications: Imaging Cancer - Correlative Microscopy - Bio-Applications: Long-term imaging using Single Plane Illumination Microscopy - Biological Processes at the Nanoscale (Celtic Session organised by MSI and SMG) - Microbial Imaging - Host-Pathogen Interactions - Bio Applications: Bacterial Ultrastructure - Electron Cryomicroscopy of Biological Macromolecules - Biological Applications of 3D Electron Microscopy Frontiers in Bioimaging Sessions - Bespoke light microscopy for molecular level imaging - Advances in labelling for super-resolution microscopy - Advanced light imaging for addressing longer length scale biological questions - Developing super-resolution methods for functional insight
Physical Sciences Sessions - Earth, Environmental and Planetary Materials - Microscopy of Engineered Surfaces and Tribology - Energy and Energy Storage Materials - Inorganic Nanomaterials (Celtic Session organised by MSI and SMG)
EMAG 2017 Keywords Instrumentation - 3-D microscopy - in-situ microscopy techniques - Low Dose Imaging and Analytical Microscopy - Dynamic TEM - Advances in SEM & FIB (CL, EBSD, beam deceleration, monochromators etc) - Detector technologies and new instrumentation - Vortex Beams and Phase plates - Electron crystallography and diffraction Materials - Biological materials, Biomaterials and Soft matter - Geological microscopy - Catalytic materials - Nanomaterials and 2D materials - Energy and Energy Storage Materials - Functional Materials - Structural Materials and Metallurgy - Surface imaging and modification - Microscopy of interfaces and heterostructures - High spatial resolution chemical and structural analysis
Scanning Probe Microscopy Sessions - Frontiers of Scanning Probe Microscopy - High Resolution SPM - Structures, Interfaces and Mechanics in Life and Health with AFM - SPM nano-mapping of materials properties
Not sure where your work would fit? You can view further details about each conference session at www.mmc-series.org.uk/conference/conference-sessions Submit your abstract at www.mmc-series.org.uk/abstracts Conference Registration is now open, book your place at www.mmc-series.org.uk/registration
Login Host: 86.152.186.215 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both hexi-at-missouri.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: hexi-at-missouri.edu Name: Xiaoqing He
Organization: Universtiy of Missouri
Title-Subject: [Filtered] Oxford INCA EDS detector dead time 100%
Message: Hi all,
We are having issues with our Oxford EDS detector on our F30. With no electron beam on, the dead time stayed at 100% no matter which dispersion rate we choose. Initially we thought the misconnection between Oxford and TEM may be responsible. But the issue persists even after we reboot Microscope PC, Oxford x-stream processor, software. etc.
Any inputs are greatly appreciated!
Thanks.
Xiaoqing He
Login Host: 161.130.188.120 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
One Gatan 794 controller is listed on E*Bay and the seller is willing to ship to Colombia - this is not free, but certainly less expensive then a new camera:
Look really hard for local people who do component level repair of complex electronics - if the problem is in controller and not the camera, then most likely it is repairable! You just need to find someone capable of such repair and willing to do it for the money you are able to pay...
Best Wishes, Valery
Valery Ray www.linkedin.com/in/valeryray ============================== PBS&T, MEO Engineering Company 290 Broadway, Suite 298 Methuen, MA 01844, USA Phone: +1-978-305-0479 - leave a message Mobie: +1-978-305-0479 - leave a message E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 2/3/2017 11:40 PM, microscopy.listserver-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: jwsandinod-at-unal.edu.co } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both jwsandinod-at-unal.edu.co as well as the } Microscopy Listserver } --------------------------------------------------------------------------- } } Email: jwsandinod-at-unal.edu.co Name: John W. Sandino } } Organization: Universidad Nacional de Colombia } } Title-Subject: [Filtered] Need Gatan 794 Camera Control } } Message: Two years ago we received an old 794 retractable camera Gatan } as donation from the Tribenberg Lab (closed). } But two days ago the camera controller of this camera does not work any } more. Today the gatan people confirm the news and told as that the } solution would buy a new camera. However we don't have budget for that. } } We ask for some who could have a camara contoller that coud give us for } free. } I know that it is no the best petition but for the moment is the unique } solution to work with our tecnai 20. one of the best microscopes in our } country Colombia. } } Thanks } Login Host: 168.176.239.31 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } }
==============================Original Headers============================== 7, 35 -- From vray-at-partbeamsystech.com Sat Feb 4 07:32:13 2017 7, 35 -- Received: from nm50-vm4.bullet.mail.bf1.yahoo.com (nm50-vm4.bullet.mail.bf1.yahoo.com [216.109.115.223]) 7, 35 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v14DWDue008165 7, 35 -- for {microscopy-at-microscopy.com} ; Sat, 4 Feb 2017 07:32:13 -0600 7, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1486215479; bh=Kvf3sCTRvNZssax2FMh6A+6/xp5xbJ0jBHlDEyhYwbU=; h=Subject:To:References:Cc:From:Date:In-Reply-To:From:Subject; b=B7tTVfyb8uaGzpNWPWoJ8AW2pnI3rp2wwMUsgRSvnZXDO8wwWhVv022GXD8lc7RGQn0roQv9BPFSkkm6wN3UKIf6LxP+eBM6qNQVh6g92BfGQoTGJmZ1L6T940CeAKBPdBnHaDwsTjptTLVKnNYh8Y7+9rmib6K5iyStTsu0Dviobc0Ry7bzKUGGc/sva2PYMzP4iPP9gP8EIJ0XNE6x99evcHQZxgWonDahTCQaU8tQX59zSxYejh5WnCJke7ffod45XVXljYcOxx69BM3GmCVLVXKqVBpuY2+vqod01ygTqIIl6T452FNKaA3y9ilBO+pUF+sFqqbQK6dnC1kkTQ== 7, 35 -- Received: from [98.139.214.32] by nm50.bullet.mail.bf1.yahoo.com with NNFMP; 04 Feb 2017 13:37:59 -0000 7, 35 -- Received: from [68.142.230.72] by tm15.bullet.mail.bf1.yahoo.com with NNFMP; 04 Feb 2017 13:37:59 -0000 7, 35 -- Received: from [127.0.0.1] by smtp229.mail.bf1.yahoo.com with NNFMP; 04 Feb 2017 13:37:59 -0000 7, 35 -- X-Yahoo-Newman-Id: 328897.38291.bm-at-smtp229.mail.bf1.yahoo.com 7, 35 -- X-Yahoo-Newman-Property: ymail-3 7, 35 -- X-YMail-OSG: Iqa8ro0VM1l3SUOY6n9JFctUwnQfL2FTxNWe8xwurJAkuLv 7, 35 -- 2jClanO7SfQiX2QIMxLHeqev.hI5WQ6Nv4U7YiFyVFKauBSd6ThU0.Prenyu 7, 35 -- WcGwwuSlfJvSPWzwMpFBCl2O9ksFRfrsE0A0Pmf1GL4z6kyXqN0FcMyjCyM7 7, 35 -- hUXwGnGSSxgPRIXq34.IMBSs8Vjmhy8T73yaUOf.8f1f8L20NDzvnjSWjsmP 7, 35 -- gr.R6qsbEiYtR0gw2HksFsZmv9F_ZgyJZ0SvG1sHRirCKMlRr4KgLaaKZmLq 7, 35 -- iIF5r6M9zxGp32bHALADFsyJAHlktplka3CvIqnpXKI.3JiO9NGYrosdjeef 7, 35 -- uaRhb3K0hWBDinO_X0goV_ouFmLFMzrFkDqA2Lr9NTULfsMh3MckiH15Hfwe 7, 35 -- t3YCH6.PHHIBq2o_uVWyj5r.ytassCl1Iv3fyl5JxyqF_AgWBveH3yKtYfiR 7, 35 -- RqcQrAwMBnliZA7zWpnl8ScrJYOUINo9F521dSESZnPcuEjCdw3yvuyDtsQP 7, 35 -- OWjKtKWbpS0TrP_5qUuSs.wVVPIN_qe._xsQRdfMpAdk52kWe8ZVTBkgzm9y 7, 35 -- p6MECMWrS 7, 35 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 7, 35 -- Subject: Re: [Microscopy] viaWWW: Need Gatan 794 Camera Controller 7, 35 -- To: jwsandinod-at-unal.edu.co 7, 35 -- References: {201702040440.v144eAf1022395-at-microscopy.com} 7, 35 -- Cc: microscopy-at-microscopy.com 7, 35 -- From: Valery Ray {vray-at-partbeamsystech.com} 7, 35 -- Message-ID: {209c6b72-69bd-ad5c-047a-60c211cbc756-at-partbeamsystech.com} 7, 35 -- Date: Sat, 4 Feb 2017 08:35:16 -0500 7, 35 -- User-Agent: Mozilla/5.0 (Windows NT 10.0; WOW64; rv:45.0) Gecko/20100101 7, 35 -- Thunderbird/45.7.0 7, 35 -- MIME-Version: 1.0 7, 35 -- In-Reply-To: {201702040440.v144eAf1022395-at-microscopy.com} 7, 35 -- Content-Type: text/plain; charset=windows-1252; format=flowed 7, 35 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Dear All, thank you very much for those who have kindly made some recommendations! I have summarized here the ones that have been recommended
1. Günter Resch (Nexperion, Vienna) 2. TipTemp 3. Extech RH520A temperature/humidity data logger 4. Vernier.com, Go Direct sensors
Hope this will be useful for the rest as well!
Cheers, Yee Yan
On Thursday, 26 January 2017, 16:51, YY YY {rongchigram79-at-yahoo.com.sg} wrote:
Dear All, i would like to ask for your recommendation for a temperature logger. Probably a reasonable sensitivity, acuracy and precision and is able to monitor live using the TEM pc. I am not sure if such system will interfere any performance of the TEM. If you encounter such problem, is it possible to share your experience? I do know some logger has wifi/bluetooth capability, will this interfere the TEM performances well? whiCurrently we have a temperature logger but its not live and we have to extract the data out from time to time which is very troublesome.
Cheers, Yee Yan, Tay FACTS Lab NTU Singapore
==============================Original Headers============================== 14, 31 -- From rongchigram79-at-yahoo.com.sg Sat Feb 4 09:48:05 2017 14, 31 -- Received: from nm4-vm3.bullet.mail.sg3.yahoo.com (nm4-vm3.bullet.mail.sg3.yahoo.com [106.10.148.130]) 14, 31 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v14Fm4qg002152 14, 31 -- for {Microscopy-at-microscopy.com} ; Sat, 4 Feb 2017 09:48:04 -0600 14, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com.sg; s=s2048; t=1486223629; bh=InN6iismgRcLjVRePsrCmyRWCaq6BX9xZsC/YqvrBzU=; h=Date:From:Reply-To:To:In-Reply-To:References:Subject:From:Subject; b=pPoSUpQM1id9HQwuYggD8s/U7Lpn6ndKZmmXExAaMrxHV7tR9adwsmSzfdfq7wgy5LMQG3P5jbtYFCZFg37TTa0V7Ga+D5E91KAweOp8VxJje2hsWZhNCiMg0rEBqnJ/KTCFbKP04kiLSPEs3JA+7dfLOkah0NagkVAEDcSetxnUcrcGnHX9xkxAvFGgKRM2/KamonrvJ7+zNG9jo5fHxoIkeTt7ymMM4eIMN7bTyp8MJMix0x3cnt/AV6Sqg8gEDUGoFeravmGHi4RLz9Vyi5rRk5vElE1FCDRZcNrMPh6nFx6kegrEhEmJet+00F2F4xnZfXjpTzvNDrfuvUcYhw== 14, 31 -- Received: from [106.10.166.126] by nm4.bullet.mail.sg3.yahoo.com with NNFMP; 04 Feb 2017 15:53:49 -0000 14, 31 -- Received: from [106.10.151.235] by tm15.bullet.mail.sg3.yahoo.com with NNFMP; 04 Feb 2017 15:53:49 -0000 14, 31 -- Received: from [127.0.0.1] by omp1019.mail.sg3.yahoo.com with NNFMP; 04 Feb 2017 15:53:49 -0000 14, 31 -- X-Yahoo-Newman-Property: ymail-3 14, 31 -- X-Yahoo-Newman-Id: 815924.4478.bm-at-omp1019.mail.sg3.yahoo.com 14, 31 -- X-YMail-OSG: xxd7uisVM1lP6tiAU0DMME8W61Dbov08AcQINWFu5CGiA_L2lw8OYEExyWl4_Sc 14, 31 -- edyDDkVe4oA6ZTVIG4CVrgBjmqMBFn_2x4EpZgRhBxPHERRqHcaXNa7HkAkdjqUvOD9sAdqNnaXB 14, 31 -- 1y48At6xvZYOlxrM.ojGxuQeCK.lciAZaflk8uhs0a0Uwgj2DRh5Iuoyw0bQ5zna6kaCs_qp0Hsp 14, 31 -- s.ddGYpr0SKxpbHUY4RlZv2GhHxPAbs9xTcp5IByEtLI3VQ.jv8OMqyxGfvca8dx8Xrqegxa6pVm 14, 31 -- tzsMJ6N4ioOXMekldlVPAE2RdalI2xTvFxQLtjHvfXLnU5a9ayjZL8XwK0TEu.ZmHT1ZpWUwQpGc 14, 31 -- gAbEfZ1j4.QL4AKqavK3pq4712puLeWzSXLdEPfknOohmlimO8ihEDnTXgpbgEbWtI._NmdAvNj7 14, 31 -- ndpXnPI1NvhpBXzyPqiNjQC2HF9lkp.V7QZwYFVI_k7ERqX.U9z2KthKfPj1QXXvVSK9PO9oGB2k 14, 31 -- MUFKWjGyccmb6D7SFmgDQzKJlXVhv_ePh8LMRpzqq0J2BiBimrBY- 14, 31 -- Received: from jws600005.mail.sg3.yahoo.com by sendmailws116.mail.sg3.yahoo.com; Sat, 04 Feb 2017 15:53:49 +0000; 1486223629.458 14, 31 -- Date: Sat, 4 Feb 2017 15:53:48 +0000 (UTC) 14, 31 -- From: YY YY {rongchigram79-at-yahoo.com.sg} 14, 31 -- Reply-To: YY YY {rongchigram79-at-yahoo.com.sg} 14, 31 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 14, 31 -- Message-ID: {1376590105.1143844.1486223628989-at-mail.yahoo.com} 14, 31 -- In-Reply-To: {1775741231.1160911.1485420702386-at-mail.yahoo.com} 14, 31 -- References: {1775741231.1160911.1485420702386.ref-at-mail.yahoo.com} {1775741231.1160911.1485420702386-at-mail.yahoo.com} 14, 31 -- Subject: Re: Temperature Logger to Recommend 14, 31 -- MIME-Version: 1.0 14, 31 -- Content-Type: text/plain; charset=UTF-8 14, 31 -- Content-Transfer-Encoding: 8bit 14, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v14Fm4qg002152 ==============================End of - Headers==============================
From stepnoah502-at-gmail.com Sun Feb 5 10:47:09 2017 Return-Path: {stepnoah502-at-gmail.com} Received: from gmail.com ([61.76.233.68]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v15Gl6IX001296 for {microscopylistserverarchive6-at-microscopy.com} ; Sun, 5 Feb 2017 10:47:08 -0600 Message-ID: {F404FFFA.5BD98938-at-gmail.com}
http://www.practicaldesign.com/THUM/thum.html
I have used two of these for over a decade to monitor T/H in microscope rooms. USB interface, so PC should be on, and data stored in a database.
Cheers,
Fred Monson
-----Original Message----- X-from: rongchigram79-at-yahoo.com.sg [mailto:rongchigram79-at-yahoo.com.sg] Sent: Saturday, February 04, 2017 10:59 AM To: Monson, Frederick {FMonson-at-wcupa.edu}
Dear All, thank you very much for those who have kindly made some recommendations! I have summarized here the ones that have been recommended
1. Günter Resch (Nexperion, Vienna) 2. TipTemp 3. Extech RH520A temperature/humidity data logger 4. Vernier.com, Go Direct sensors
Hope this will be useful for the rest as well!
Cheers, Yee Yan
On Thursday, 26 January 2017, 16:51, YY YY {rongchigram79-at-yahoo.com.sg} wrote:
Dear All, i would like to ask for your recommendation for a temperature logger. Probably a reasonable sensitivity, acuracy and precision and is able to monitor live using the TEM pc. I am not sure if such system will interfere any performance of the TEM. If you encounter such problem, is it possible to share your experience? I do know some logger has wifi/bluetooth capability, will this interfere the TEM performances well? whiCurrently we have a temperature logger but its not live and we have to extract the data out from time to time which is very troublesome.
Cheers, Yee Yan, Tay FACTS Lab NTU Singapore
==============================Original Headers============================== 14, 31 -- From rongchigram79-at-yahoo.com.sg Sat Feb 4 09:48:05 2017 14, 31 -- Received: from nm4-vm3.bullet.mail.sg3.yahoo.com (nm4-vm3.bullet.mail.sg3.yahoo.com [106.10.148.130]) 14, 31 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v14Fm4qg002152 14, 31 -- for {Microscopy-at-microscopy.com} ; Sat, 4 Feb 2017 09:48:04 -0600 14, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com.sg; s=s2048; t=1486223629; bh=InN6iismgRcLjVRePsrCmyRWCaq6BX9xZsC/YqvrBzU=; h=Date:From:Reply-To:To:In-Reply-To:References:Subject:From:Subject; b=pPoSUpQM1id9HQwuYggD8s/U7Lpn6ndKZmmXExAaMrxHV7tR9adwsmSzfdfq7wgy5LMQG3P5jbtYFCZFg37TTa0V7Ga+D5E91KAweOp8VxJje2hsWZhNCiMg0rEBqnJ/KTCFbKP04kiLSPEs3JA+7dfLOkah0NagkVAEDcSetxnUcrcGnHX9xkxAvFGgKRM2/KamonrvJ7+zNG9jo5fHxoIkeTt7ymMM4eIMN7bTyp8MJMix0x3cnt/AV6Sqg8gEDUGoFeravmGHi4RLz9Vyi5rRk5vElE1FCDRZcNrMPh6nFx6kegrEhEmJet+00F2F4xnZfXjpTzvNDrfuvUcYhw== 14, 31 -- Received: from [106.10.166.126] by nm4.bullet.mail.sg3.yahoo.com with NNFMP; 04 Feb 2017 15:53:49 -0000 14, 31 -- Received: from [106.10.151.235] by tm15.bullet.mail.sg3.yahoo.com with NNFMP; 04 Feb 2017 15:53:49 -0000 14, 31 -- Received: from [127.0.0.1] by omp1019.mail.sg3.yahoo.com with NNFMP; 04 Feb 2017 15:53:49 -0000 14, 31 -- X-Yahoo-Newman-Property: ymail-3 14, 31 -- X-Yahoo-Newman-Id: 815924.4478.bm-at-omp1019.mail.sg3.yahoo.com 14, 31 -- X-YMail-OSG: xxd7uisVM1lP6tiAU0DMME8W61Dbov08AcQINWFu5CGiA_L2lw8OYEExyWl4_Sc 14, 31 -- edyDDkVe4oA6ZTVIG4CVrgBjmqMBFn_2x4EpZgRhBxPHERRqHcaXNa7HkAkdjqUvOD9sAdqNnaXB 14, 31 -- 1y48At6xvZYOlxrM.ojGxuQeCK.lciAZaflk8uhs0a0Uwgj2DRh5Iuoyw0bQ5zna6kaCs_qp0Hsp 14, 31 -- s.ddGYpr0SKxpbHUY4RlZv2GhHxPAbs9xTcp5IByEtLI3VQ.jv8OMqyxGfvca8dx8Xrqegxa6pVm 14, 31 -- tzsMJ6N4ioOXMekldlVPAE2RdalI2xTvFxQLtjHvfXLnU5a9ayjZL8XwK0TEu.ZmHT1ZpWUwQpGc 14, 31 -- gAbEfZ1j4.QL4AKqavK3pq4712puLeWzSXLdEPfknOohmlimO8ihEDnTXgpbgEbWtI._NmdAvNj7 14, 31 -- ndpXnPI1NvhpBXzyPqiNjQC2HF9lkp.V7QZwYFVI_k7ERqX.U9z2KthKfPj1QXXvVSK9PO9oGB2k 14, 31 -- MUFKWjGyccmb6D7SFmgDQzKJlXVhv_ePh8LMRpzqq0J2BiBimrBY- 14, 31 -- Received: from jws600005.mail.sg3.yahoo.com by sendmailws116.mail.sg3.yahoo.com; Sat, 04 Feb 2017 15:53:49 +0000; 1486223629.458 14, 31 -- Date: Sat, 4 Feb 2017 15:53:48 +0000 (UTC) 14, 31 -- From: YY YY {rongchigram79-at-yahoo.com.sg} 14, 31 -- Reply-To: YY YY {rongchigram79-at-yahoo.com.sg} 14, 31 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 14, 31 -- Message-ID: {1376590105.1143844.1486223628989-at-mail.yahoo.com} 14, 31 -- In-Reply-To: {1775741231.1160911.1485420702386-at-mail.yahoo.com} 14, 31 -- References: {1775741231.1160911.1485420702386.ref-at-mail.yahoo.com} {1775741231.1160911.1485420702386-at-mail.yahoo.com} 14, 31 -- Subject: Re: Temperature Logger to Recommend 14, 31 -- MIME-Version: 1.0 14, 31 -- Content-Type: text/plain; charset=UTF-8 14, 31 -- Content-Transfer-Encoding: 8bit 14, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v14Fm4qg002152 ==============================End of - Headers==============================
==============================Original Headers============================== 24, 41 -- From FMonson-at-wcupa.edu Mon Feb 6 14:57:45 2017 24, 41 -- Received: from MX01.WCUPA.EDU (mx01.wcupa.edu [144.26.63.2]) 24, 41 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v16KvjIF017282 24, 41 -- for {microscopy-at-microscopy.com} ; Mon, 6 Feb 2017 14:57:45 -0600 24, 41 -- X-ASG-Debug-ID: 1486415019-08911c2a59823d60001-4CH8be 24, 41 -- Received: from WCUXCHP02.PASSHE.LCL ([144.26.2.138]) by MX01.WCUPA.EDU with ESMTP id HXluSUbrlLVvkHGs (version=TLSv1.2 cipher=ECDHE-RSA-AES256-SHA384 bits=256 verify=NO); Mon, 06 Feb 2017 16:03:39 -0500 (EST) 24, 41 -- X-Barracuda-Envelope-From: FMonson-at-wcupa.edu 24, 41 -- X-Barracuda-Apparent-Source-IP: 144.26.2.138 24, 41 -- X-ASG-Whitelist: Client 24, 41 -- Received: from WCUXCHP08.PASSHE.LCL (144.26.2.144) by WCUXCHP02.PASSHE.LCL 24, 41 -- (144.26.2.138) with Microsoft SMTP Server (TLS) id 15.0.1178.4; Mon, 6 Feb 24, 41 -- 2017 16:02:56 -0500 24, 41 -- Received: from WCUXCHP08.PASSHE.LCL ([144.26.2.144]) by WCUXCHP08.PASSHE.LCL 24, 41 -- ([144.26.2.144]) with mapi id 15.00.1178.000; Mon, 6 Feb 2017 16:02:56 -0500 24, 41 -- From: "Monson, Frederick" {FMonson-at-wcupa.edu} 24, 41 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 24, 41 -- CC: "rongchigram79-at-yahoo.com.sg" {rongchigram79-at-yahoo.com.sg} 24, 41 -- Subject: RE: [Microscopy] Re: Temperature Logger to Recommend 24, 41 -- Thread-Topic: [Microscopy] Re: Temperature Logger to Recommend 24, 41 -- X-ASG-Orig-Subj: RE: [Microscopy] Re: Temperature Logger to Recommend 24, 41 -- Thread-Index: AQHSfwBcAS2bYFzvIUe8JTzF+vus06FceloQ 24, 41 -- Date: Mon, 6 Feb 2017 21:02:56 +0000 24, 41 -- Message-ID: {9cec4e3d33244e649868e83a17bbf094-at-WCUXCHP08.PASSHE.LCL} 24, 41 -- References: {201702041559.v14FxIjV012791-at-microscopy.com} 24, 41 -- In-Reply-To: {201702041559.v14FxIjV012791-at-microscopy.com} 24, 41 -- Accept-Language: en-US 24, 41 -- Content-Language: en-US 24, 41 -- X-MS-Has-Attach: 24, 41 -- X-MS-TNEF-Correlator: 24, 41 -- x-ms-exchange-transport-fromentityheader: Hosted 24, 41 -- x-originating-ip: [144.26.2.58] 24, 41 -- Content-Type: text/plain; charset="iso-8859-1" 24, 41 -- MIME-Version: 1.0 24, 41 -- X-Barracuda-Connect: UNKNOWN[144.26.2.138] 24, 41 -- X-Barracuda-Start-Time: 1486415019 24, 41 -- X-Barracuda-Encrypted: ECDHE-RSA-AES256-SHA384 24, 41 -- X-Barracuda-URL: https://SPAMCONTROL.WCUPA.EDU:443/cgi-mod/mark.cgi 24, 41 -- X-Virus-Scanned: by bsmtpd at WCUPA.EDU 24, 41 -- X-Barracuda-BRTS-Status: 1 24, 41 -- Content-Transfer-Encoding: 8bit 24, 41 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v16KvjIF017282 ==============================End of - Headers==============================
From marilore504-at-gmail.com Mon Feb 6 18:14:02 2017 Return-Path: {marilore504-at-gmail.com} Received: from gmail.com ([112.121.188.90]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v170DxpG016138 for {microscopylistserverarchive6-at-microscopy.com} ; Mon, 6 Feb 2017 18:14:01 -0600 Message-ID: {9DCA92EA.3D42EAF7-at-gmail.com}
X-from: mikeh-at-ncimicro.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mikeh-at-ncimicro.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: mikeh-at-ncimicro.com Name: mike hehr
Organization: NCI
Title-Subject: [Filtered] EM Sample Preparation Sales Position
Message: Scientific Instrument distributor looking for a Nano Technology Professional to sell Electron Microscopy sample preparation equipment in the Midwest. The ideal candidate will have experience in Electron Micorscopy and knowledge of the various instruments used to perform the sample prep. These instruments include: Scanning Electron Microscopes, Ion Millers, Ultra Microtomes, High Pressure Freezers, Critical Point Dryers, Coaters, Knife Makers and other.
This is a highly consultative sales process that requires extensive knowledge with EM sample preparation but also the winning spirit to win the sale and train users on the equipment / techniques for successful preparation.
Sales Experience preferred, but not needed for the right candidate.
Salary, Commission, Benefits
If interested, Please send you resume to mikeh-at-ncimicro.com
Login Host: 23.25.147.133 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- ============================================= Do no rely to this messaage it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
From ronaldfaust205-at-gmail.com Wed Feb 8 06:27:11 2017 Return-Path: {ronaldfaust205-at-gmail.com} Received: from gmail.com ([218.232.94.109]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v18CR8Eb016233 for {microscopylistserverarchive6-at-microscopy.com} ; Wed, 8 Feb 2017 06:27:10 -0600 Message-ID: {CA32B257.17A4C6A8-at-gmail.com}
Dear Listers,
I am wondering if anyone may have installation/service manual for Jeol JEM 2100F TEM, or any notes related to its installation you would be willing to share. I have full operation manual with the instrument, but need to move it.
Thank you very much beforehand, -- Valery Ray www.linkedin.com/in/valeryray ============================== PBS&T, MEO Engineering Company 290 Broadway, Suite 298 Methuen, MA 01844, USA Phone: +1-978-305-0479 - leave a message Mobie: +1-978-305-0479 - leave a message E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
==============================Original Headers============================== 3, 32 -- From vray-at-partbeamsystech.com Wed Feb 8 11:38:14 2017 3, 32 -- Received: from nm33-vm2.bullet.mail.bf1.yahoo.com (nm33-vm2.bullet.mail.bf1.yahoo.com [72.30.239.202]) 3, 32 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v18HcE2t030983 3, 32 -- for {microscopy-at-microscopy.com} ; Wed, 8 Feb 2017 11:38:14 -0600 3, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1486575855; bh=fvsEZMoYYtRd/522f209rbVhdq+t48xujhCZFPRR7WQ=; h=To:From:Subject:Date:From:Subject; b=iyV1YabkoUNM6osVVKm3+sqQf5Ki9lp161ym4XjCCdv99y31UONo7bQETADFH8Tk+yJ30IoLM5KjM6Jc/uz6cCfasI6M8zC5NvqmYGY4e7tIs+LhMvo6sjdL6hWtD14RBPTb654JuXzDodzI5C10evm48Y/pIFQSJ0Yl2BN76hyN+fJmgnTkijFQF2Vsmn1A+MNTpveSGvIe0IZKqkdgZVTi71FewLHver8hd4PKfNY3GLm4WDiKiL7xQLeEDR6ULhHDacLU//voNSfXi6+myTlWYDXTyKSjuLYeaYAP69xaFQI8KNim22YrqEz4w0OJlDiB9Xk+ynff3hVcfA2bfA== 3, 32 -- Received: from [98.139.170.179] by nm33.bullet.mail.bf1.yahoo.com with NNFMP; 08 Feb 2017 17:44:15 -0000 3, 32 -- Received: from [98.139.213.12] by tm22.bullet.mail.bf1.yahoo.com with NNFMP; 08 Feb 2017 17:44:15 -0000 3, 32 -- Received: from [127.0.0.1] by smtp112.mail.bf1.yahoo.com with NNFMP; 08 Feb 2017 17:44:15 -0000 3, 32 -- X-Yahoo-Newman-Id: 770504.57260.bm-at-smtp112.mail.bf1.yahoo.com 3, 32 -- X-Yahoo-Newman-Property: ymail-3 3, 32 -- X-YMail-OSG: riGx0j0VM1m7Yf2a6kjjeLr1jv2oht9zW6c.q_cxyLSLz52 3, 32 -- 274NDKbndNbR9EC1nmIeRknvN4uKMMKN.qo3yA_VblfKEwe5AYg.T1KfVgMJ 3, 32 -- R1F0hyR.bfUGaGHaJx.Zn_jpqVTqn5LimQcKwaLAla6QTy6xEF4v_ftkJgQl 3, 32 -- nOtxSU1SrYwASFViGyORYYKBAGx2.1c5o0DuVfONnMszvO48UvuMBZYJPEos 3, 32 -- jyMTQLApejwNTbCe3df7cnV82pSLCZH3F8oE9.b0uF4.jIGszfrDAS2AIGT5 3, 32 -- .kiHTL24HyDOVnQxCwcoyLG7mWNwUPOle3_UwYzw.LENrhX8qhKiQCzhJp5J 3, 32 -- zUdN8l5WygP_63AtVeT7yPbbmr0TnkMb0uQBMFOXomMXaY.Gw7Jk_C6KQWOh 3, 32 -- dQdSsFr.K8Ne7s_PxEZPpinl2P1CsYY9liRIYoMkFzB.kJQDZzwPfZVo_BoJ 3, 32 -- AZiS3u6N_54Z.VI07KVegzsgFWNTKSDN.FBHze5rHP25V6CaUk.iqolA6OxE 3, 32 -- 4.bjyggOkkY.8aCyrVCFtCW4K3ZKjdxoB.lBJMDWybJSSctri1OQmLJVFO2l 3, 32 -- _c5Iz5vHS 3, 32 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 3, 32 -- To: microscopy-at-microscopy.com 3, 32 -- From: Valery Ray {vray-at-partbeamsystech.com} 3, 32 -- Subject: JEOL JEM 2100F installation/service manual? 3, 32 -- Message-ID: {b0f3dd83-a62f-6d65-0939-b693462463c9-at-partbeamsystech.com} 3, 32 -- Date: Wed, 8 Feb 2017 12:41:25 -0500 3, 32 -- User-Agent: Mozilla/5.0 (Windows NT 10.0; WOW64; rv:45.0) Gecko/20100101 3, 32 -- Thunderbird/45.7.0 3, 32 -- MIME-Version: 1.0 3, 32 -- Content-Type: text/plain; charset=utf-8; format=flowed 3, 32 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both gavramo-at-fas.harvard.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: gavramo-at-fas.harvard.edu Name: Gil Avramovich
Organization: Harvard University Department of Molecular and Cell Biology
Title-Subject: [Filtered] EM Microwave Tissue Processing Workshop
Message: Message: RSVP now for an advanced Microwave Tissue Processing Workshop for applications in Electron Microscopy, which will be held on the Cambridge campus of Harvard University, room 254 in NW Labs, 52 Oxford St, Cambridge MA 02138. Join us on February 14th and 15th, and enjoy complimentary food and coffee, and enjoy learning about cutting edge technology alongside the foremost experts in Electron Microscopy. The workshop will cover: Þ Microwave Technology in Research, Þ Sample Preparation for Microwave Processing, Þ Microwave Processing for Electron Microscopy, Þ Electron Microscopy Polymerization, and Þ Alternative applications and associated kits. The workshop is totally free, and spots are limited, so please send your RSVP to gavramo-at-fas.harvard.edu by FEBRUARY 11th in order to sign up.
Login Host: 140.247.107.22 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both rms-at-angstrom.us as well as the Microscopy Listserver ---------------------------------------------------------------------------
Message: Position: Technical Sales Representative - Mid Atlantic US Territory: Dependent on the candidate but will include MD, DE, DC, VA, NJ & PA. Products: Sales and support of the following products/companies: Hitachi: Bench-top Scanning Electron Microscopes (SEM) and AFM Kleindiek: Nano-manipulators, Probe Stations and Stages Leica: Electron Microscopy sample prep. equipment EMSIS TEM Cameras Deben Tensile Stages and Microscope AccessoriesJ V/Bruker Semi: X-ray Diffraction (XRD)
Position Description: This is an exciting position selling and supporting a range of products in for a number of leading companies in the electron microscopy and related markets. The ideal candidate will preferably have a demonstrated track record of success (2-3 years) selling technical products into the academic, research and industrial and/or life sciences markets. Optical microscopy or SEM/TEM experience a definite plus. Academic background should be at min. an associates degree in science (BS or masters preferred). The successful candidate will live in one of the above listed states. Angstrom Scientific Inc. is a growing distributor of products targeted to the nanotechnology, research, life science and semiconductor markets with exciting new and novel technology. The recent addition of Leica with its impressive portfolio of EM sample prep. equipment complements and strengthens our position in this region. Angstrom Scientific Inc. offers competitive salary and benefits, commission, car allowance and expenses. We are an equal opportunity employer. Interested candidates should submit their resume to: Bob Sommerville, CEO, Angstrom Scientific Inc. e-mail: rms-at-angstrom.us
Keywords: Scanning Electron Microscope, SEM, TEM, EM Sample Preparation, X-Ray, XRD, Nano-Manipulators, Probing.
Login Host: 47.16.89.121 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- ============================================= Do no rely to this messaage it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
Hello, It could be that you have different grounding points in use, and an electric potential between them causing a ground current being the issue. You might want to check that your ground lines are correctly wired.
The general recommendation is to use the frame of the TEM as the only grounding point for your TEM connected accessories, arranging a grounding bus at the frame and avoiding any ground loops between the accessories. For instance, do not use the house grounding line feeding to the power socket which powers the EDX equipment and EDX computer. The house grounding line stays disconnected from this power socket. This socket's grounding (not the house grounding line) becomes linked to the frame of the TEM, instead. If I remember correctly, then Oxford ships an accordingly manipulated distributor with their equipment for easing such wiring. Don't forget to also connect the chassis of the EDX computer to your grounding point at the frame of the TEM. Consult a certified electrician for assuring electrics security in your setup!
Best greetings from the EM-Labs of CIC biomaGUNE (Spain), Marco Mller
} } Email: hexi-at-missouri.edu Name: Xiaoqing He } } Organization: Universtiy of Missouri } } Title-Subject: [Filtered] Oxford INCA EDS detector dead time 100% } } Message: Hi all, } } We are having issues with our Oxford EDS detector on our F30. With no } electron beam on, the dead time stayed at 100% no matter which } dispersion rate we choose. Initially we thought the misconnection } between Oxford and TEM may be responsible. But the issue persists even } after we reboot Microscope PC, Oxford x-stream processor, software. } etc. } } Any inputs are greatly appreciated! } } Thanks. } } Xiaoqing He
==============================Original Headers============================== 6, 46 -- From mmoller-at-cicbiomagune.es Thu Feb 9 02:21:04 2017 6, 46 -- Received: from webmail.cicbiomagune.es (webmail.cicbiomagune.es [150.241.252.34]) 6, 46 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v198L3Dr031690 6, 46 -- for {Microscopy-at-microscopy.com} ; Thu, 9 Feb 2017 02:21:04 -0600 6, 46 -- X-AuditID: 0a0d0165-ff5ff70000007f79-8f-589c27d7c066 6, 46 -- Received: from cicmgex1.cicbiomagune.int (cicmgex1.cicbiomagune.int [10.10.1.98]) 6, 46 -- by webmail.cicbiomagune.es (Symantec Messaging Gateway) with SMTP id 34.E1.32633.7D72C985; Thu, 9 Feb 2017 09:27:05 +0100 (CET) 6, 46 -- Received: from cicmgex1.cicbiomagune.int (10.10.1.98) by 6, 46 -- cicmgex1.cicbiomagune.int (10.10.1.98) with Microsoft SMTP Server (TLS) id 6, 46 -- 15.0.1210.3; Thu, 9 Feb 2017 09:27:03 +0100 6, 46 -- Received: from CICMGEXCAS.cicbiomagune.int (10.10.1.3) by 6, 46 -- cicmgex1.cicbiomagune.int (10.10.1.98) with Microsoft SMTP Server (TLS) id 6, 46 -- 15.0.1210.3 via Frontend Transport; Thu, 9 Feb 2017 09:27:02 +0100 6, 46 -- Received: from CICMGEXMBX.cicbiomagune.int ([::1]) by 6, 46 -- CICMGEXCAS.cicbiomagune.int ([::1]) with mapi id 14.03.0195.001; Thu, 9 Feb 6, 46 -- 2017 09:27:02 +0100 6, 46 -- From: Marco Moller {mmoller-at-cicbiomagune.es} 6, 46 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} , 6, 46 -- "hexi-at-missouri.edu" {hexi-at-missouri.edu} 6, 46 -- Subject: RE: Oxford INCA EDS detector dead time 100% 6, 46 -- Thread-Topic: Oxford INCA EDS detector dead time 100% 6, 46 -- Thread-Index: AQHSgq5J87TxHrm1zUCxCnJ2dAce0A== 6, 46 -- Date: Thu, 9 Feb 2017 08:27:02 +0000 6, 46 -- Message-ID: {216351DE18DE144BBBFC1996E5FAEBB204E9F2-at-CICMGEXMBX.cicbiomagune.int} 6, 46 -- References: {201702040502.v1452nBu002756-at-microscopy.com} 6, 46 -- Accept-Language: de-DE, es-ES, en-US 6, 46 -- Content-Language: en-US 6, 46 -- X-MS-Has-Attach: 6, 46 -- X-MS-TNEF-Correlator: 6, 46 -- x-originating-ip: [10.10.2.151] 6, 46 -- Content-Type: text/plain; charset="iso-8859-1" 6, 46 -- MIME-Version: 1.0 6, 46 -- X-Brightmail-Tracker: H4sIAAAAAAAAA+NgFuplkeLIzCtJLcpLzFFi42Lh4mJM0r2pPifCYOsPPYvL5+8zWvw53MDu 6, 46 -- wOTxsfs1k0fPzOAApigum5TUnMyy1CJ9uwSujLvnb7MXrOSr+LboCFsD42nuLkZODgkBE4n3 6, 46 -- jy6ydTFycQgJXGGUOHZjIzNIQkhgM6NE09kiiMRpRokNJ86zQDgzGSUa185iAaliE9CReHT6 6, 46 -- OpgtIpAmMen5XLBuYaCxP+7MZ4WIm0o0rZ7DBGHrSdx7vo4NxGYRUJG48H06WC+vQIDEqcbt 6, 46 -- TBCbLSW6H0wBsjk4GIFqNrzSBQkzC4hL3HoynwniagGJJXvOM0PYohIvH/9jBSmXEJCXuDXN 6, 46 -- EaJcT+LG1ClsELa2xLKFr5khNglKnJz5hAVik4rE7jcr2Ccwis1CsmEWkvZZSNpnIWlfwMiy 6, 46 -- ilE6qSgzPaMkNzEzRy85MzkpMz83Mb00L1UPGFGbGEGRxMuYuoNx/0TdQ4wCHIxKPLwVlrMj 6, 46 -- hFgTy4orcw8xSnAwK4nwFirPiRDiTUmsrEotyo8vKs1JLT7EKM3BoiTOm+VTFyEkkJ5Ykpqd 6, 46 -- mlqQWgSTZeLglGpgZA56/0zyrEQK95Xt/Wcv8apsZuy49NUgMij0hdipNzOjE6p/ZeXwzGB6 6, 46 -- FpR++fWrVwU7nn268mhFya2sp241H0qTwnlXuipcZdCcVTTp8ssW8w0nYoT79ly8GbSiWHlK 6, 46 -- WXtxtaxSj33VCuvPdfu4mlLTVmrp7d4ddXWD4H8W+5VXpaurQmxmKrEUZyQaajEXFScCAJj+ 6, 46 -- vRagAgAA 6, 46 -- Content-Transfer-Encoding: 8bit 6, 46 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v198L3Dr031690 ==============================End of - Headers==============================
From pedugjuf5-at-gmail.com Thu Feb 9 05:44:48 2017 Return-Path: {pedugjuf5-at-gmail.com} Received: from gmail.com (lync-mediation.hosted.chinatelecomglobal.com [203.14.188.88]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v19BijCi029503 for {microscopylistserverarchive6-at-microscopy.com} ; Thu, 9 Feb 2017 05:44:47 -0600 Message-ID: {192DB78B.827FA1FC-at-gmail.com}
X-from: thomas.horn-at-bsse.ethz.ch
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both thomas.horn-at-bsse.ethz.ch as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: thomas.horn-at-bsse.ethz.ch Name: Thomas Horn
Organization: ETH Zurich
Title-Subject: [Filtered] Position open in Basel, Switzerland: Microscopy Engineer/Microscopist
Message: Dear all, we have an open position at the Single Cell Facility , ETH, Department of Biosystems Science and Engineering (D-BSSE), Basel: Microscopy Engineer / Microscopist (m/f) Responsibilities are training and support of facility users. This includes microscopy instrument quality control and their maintenance. The microscopy engineer/microscopist will develop and implement customized configurations and the setup of the experimental workflows of experiments on the automated imaging systems. The successful candidate will share responsibilities with our other microscopy expert and work closely together with our software engineer for image analysis and data management. This position is available for 2 years and located in Basel. Requirements are a degree from a technical college or a university with a proven experience in various advanced light microscopy and digital imaging technologies. She/he will have extensive technical experience with state-of-the-art light microscopy platforms such as confocal, live-cell imaging and/or SPIM. Strong knowledge of advanced quantitative imaging technologies as well as their underlying optical principles is required. Scripting experience is a plus. The candidate should have hands-on skills in microscopy component integration. This position demands very good communication skills in English to interact with scientists and students, and the skill to adapt to the service character of the facility to successfully support scientific research and operations at the D-BSSE. Besides having excellent technical abilities in the field of light microscopy, the candidate must be an excellent team player and possess the ability to work in a variety of scientific areas. The Single Cell Facility (SCF) is a central scientific core facility within the ETH Zurich, Department of Biosystems Science and Engineering (D-BSSE), located in Basel. The SCF serves the department by providing technology and support in advanced (light-sheet, automated wide field, confocal) microscopy and flow cytometry. For further information about the position, please contact Dr. Thomas Horn, by email: thomas.horn-at-bsse.ethz.ch (no applications).
Applications are only accepted online, please use the Apply now button at the link below. Please address it to: ETH Zurich, Roland Munz, Human Resources, CH-8092 Zurich.
Further information on our facility: https://www.bsse.ethz.ch/scf
Best regards, Thomas Horn. Login Host: 129.132.229.196 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jhyun-at-gatan.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: jhyun-at-gatan.com Name: John Hyun
Organization: Gatan
Title-Subject: [Filtered] EELS Training School - April 2017, California
Message: EELS & EFTEM Analysis Training School
April 4-7, 2017, Gatan R&D Headquarters, Pleasanton , CA
Electron energy loss spectroscopy (EELS) is a powerful technique that provides both compositional and chemical information from sub-nanometer areas in the sample. As a course attendee, you will learn best practices to set up and optimize your EELS hardware and experimental protocols so you can capture and extract the maximum amount of compositional and chemical information from your TEM samples. Topics include:
Fundamentals of EELS and energy-filtered imaging in TEM Principles of operation of Gatan EFTEM and EELS systems Optimization of EFTEM and EELS data acquisition Quantification of elemental composition Other information provided by EFTEM/EELS and how best to extract it Use of EELS signals to form maps of elemental and chemical composition EFTEM and STEM EELS spectrum imaging techniques Identification of material phases via EELS fine structure mapping Applications to biological and physical science specimens
School details and online registration: http://www.gatan.com/company/events/eels-eftem-analysis-training-school-april-2017
Login Host: 50.58.128.11 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- ============================================= Do no rely to this messaage it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
From shkrsugi29-at-gmail.com Sun Feb 12 11:00:56 2017 Return-Path: {shkrsugi29-at-gmail.com} Received: from gmail.com ([112.121.167.82]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v1CH0r1q017848 for {microscopylistserverarchive6-at-microscopy.com} ; Sun, 12 Feb 2017 11:00:55 -0600 Message-ID: {27532BAF.4AEABBE4-at-gmail.com}
Hi,
We have an old Gauss Maus (Dindima Group/Arlunya) , a hand held device for measuring magnetic fields. We need to work out the magnitude and direction of some stray fields that have just appeared (intermittently of course) in our lab but unfortunately have lost the manual. I have searched on the internet but couldn't find a manual there. If anyone has a copy of the manual they could scan and email to me or, send to me so that I can scan it and send it back it would be much appreciated.
E colin.veitch-at-csiro.au T +61 3 5246 4891 M 0438 538 475 F +61 3 5246 4057 Address CSIRO Manufacturing, 75 Pigdons Road, Waurn Ponds, Vic 3216, Australia. www.csiro.au | http://www.csiro.au/Organisation-Structure/Flagships/Manufacturing.aspx
PLEASE NOTE The information contained in this email may be confidential or privileged. Any unauthorised use or disclosure is prohibited. If you have received this email in error, please delete it immediately and notify the sender by return email. Thank you. To the extent permitted by law, CSIRO does not represent, warrant and/or guarantee that the integrity of this communication has been maintained or that the communication is free of errors, virus, interception or interference.
Please consider the environment before printing this email.
E colin.veitch-at-csiro.au T +61 3 5246 4891 M 0438 538 475 F +61 3 5246 4057 Address CSIRO Manufacturing, 75 Pigdons Road, Waurn Ponds, Vic 3216, Australia. www.csiro.au | http://www.csiro.au/Organisation-Structure/Flagships/Manufacturing.aspx
PLEASE NOTE The information contained in this email may be confidential or privileged. Any unauthorised use or disclosure is prohibited. If you have received this email in error, please delete it immediately and notify the sender by return email. Thank you. To the extent permitted by law, CSIRO does not represent, warrant and/or guarantee that the integrity of this communication has been maintained or that the communication is free of errors, virus, interception or interference.
Please consider the environment before printing this email.
Last spring, a thread was started on the quality/longevity of newer LaB6 cathodes. I looked back through the e-mails and it seemed like the last thing that was mentioned was something about ongoing discussions with the vendors.
I have some grant money left that I need to spend soon and was thinking of switching our CM100 over from W to LaB6. I worry, however, that it may not be worth it since it seemed like others were having problems with the longevity of the cathodes.
I did contact the original person who started the thread on this listserv but have yet to receive a reply. Does anyone know what the status of the cathodes is?
Thank you, -Blanca ----------------------------------------- Blanca Carbajal Gonzalez, M.S. Director of Microscopy Science Center 50 College St Mount Holyoke College Clapp Laboratory 123 Office: 413-538-3118 Cell: 559-905-7138 bicarbaj-at-mtholyoke.edu MHC Microscopy
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both fengxia.liang-at-med.nyu.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: fengxia.liang-at-med.nyu.edu Name: Alice Liang
Organization: New York University Langone Medical Center
Title-Subject: [Filtered] CryoEM manager-NYU
Message: Cryo EM Manager Position at NYU Langone School of Medicine
NYU Langone School of Medicine is seeking a cryo-EM manager to oversee the operation of our expanding, state-of-the- art electron microscopy facility. The facility will include Titan Krios and Talos Arctica microscopes, both with direct detectors, for single particle EM and cryo electron tomography. The facility will be staffed by 3 team members, including the manager (this position) and two junior associates. The facility manager will be responsible for oversight and maintenance of these microscopes as well as training and assisting users, who will be primarily from the NYU research community. The manager will interface with our High Performance Computing team to ensure a seamless interface between data collection and data processing. The manager will also have the opportunity to, and will be encouraged to interact with cryo EM experts in the New York area and to attend key EM conferences (e.g. EM Gordon Conference) in order to integrate NYU with the greater EM community, and to implement cutting-edge techniques and technologies into our facility.
Responsibilities (together with 2 other team members): Maintain Talos Arctica and Krios microscopes. This duty requires interfacing with FEI engineers to ensure optimal performance of the microscopes. Maintain ancillary, cryo-EM related equipment such as Vitrobot, glow discharger etc. Load samples and align microscopes prior to data collection Train and oversee users for data collection Evaluate quality of data obtained by users Interface and collaborate with High Performance Computing team, who will assist with computational needs for optimal data collection, storage and data processing Interface and collaborate with Microscopy Core, which houses electron microscopes for preliminary screening of samples Implement data collection strategies that will benefit our user community Maintain accurate logs for microscope performance and usage
Key element of the job: This facility will be optimized for high throughput data collection. Developing a pipeline that fits well with our users will be a key part of this job. Although the manager is expected to consult with faculty supervisors, he/she will be given flexibility and responsibility to develop a pipeline that generates high quality data in an efficient manner.
Requirements: PhD in structural biology, biochemistry or related field At least 2 years of cryo EM experience, with expertise in handling samples, using microscopes and evaluating images Excellent communication and interpersonal skills Knowledge of the principles of transmission electron microscopy General understanding of single particle cryo EM workflow; experience in cryo electron tomography is a plus Special consideration will be given to candidates with management experience or a record of achievement in collaborative, team-driven environments
The Ideal Candidate: In addition to meeting all the requirements above, the ideal candidate will also bring enthusiasm and passion to developing our new facility. We are looking for a colleague who will be excited about interacting with researchers at all levels and will consistently strive to maintain a state-of-the-art facility. The EM manager will be an key component of our new cryo EM initiative, and as such, will play a crucial role in shaping the new facility. This job is an excellent opportunity for an electron microscopist who is interested in a leadership role within the vibrant research community at NYU School of Medicine.
Salary will be competitive and job title will depend on qualifications of the candidate.
To apply for this position, please email cryoemnyu-at-gmail.com and include: 1) Cover letter 2) CV 3) Contact information for 3 references
Login Host: 216.165.126.103 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
From stepnoah502-at-gmail.com Wed Feb 15 13:56:35 2017 Return-Path: {stepnoah502-at-gmail.com} Received: from gmail.com (static.vdc.vn [113.160.108.75] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v1FJuWcu027444 for {microscopylistserverarchive6-at-microscopy.com} ; Wed, 15 Feb 2017 13:56:34 -0600 Message-ID: {FB6D4791.5BCEEE94-at-gmail.com}
We need to remove the top of our Leica Ultracut S ultramicrotome to check something on the electrical circuit for the overhead light source. I am talking about the top plate that carries the binocular microscope that allows you to view the specimen and cutting action.
It looks like there is a screw at the very back of this plate that can be seen from the underside when the plate is rotated 90 degrees. I am guessing that one removes that screw and then you can used the normal hand driven gear drive to move the carrier forward until it is free of the underlying dovetail. Can anyone confirm this. Additionally, where does one disconnect the electrical to the fluorescent light source? Will I see a plug once I remove this screw and move the carrier forward?
Thanks, Tom
Thomas E. Phillips, Ph.D Curator's Distinguished Teaching Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
Thomas E. Phillips, Ph.D Curator's Distinguished Teaching Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both roger.ristau-at-uconn.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: roger.ristau-at-uconn.edu Name: Roger Ristau
Organization: University of Connecticut
Title-Subject: [Filtered] FIB STEM EDX
Message: A question for anyone with FIB-STEM-EDX system: Our FEI Helios 460F1 has single-sample Flip Stage (the 'turret' type Flip Stage with 180 deg rotation) and STEM detector for imaging TEM liftouts in situ. When attempting to acquire EDX of the lifout, mounted on the Flip Stage, using EDAX Octane Plus SDD EDX, the spectrum is overwhelmed by the Si peak from the STEM detector itself. If the STEM detector is retracted, the Si peak is replaced by a Al peak from the stage area situated below the STEM position.
Obviously the EDX detector is 'seeing' the X-rays generated by the e-beam that passes through the thin liftout sample. However, this problem did not occur in our older Strata FIB with SiLi detector. I am presuming it may have something to do with the larger collection angle of the SDD. I have confirmed with EDAX that the detector is installed correctly,the collimator is in position and the system is operating normally. (There are no problems doing EDX with the sample in the 'bulk stage' position.)
My temporary solution is to affix a strip of carbon tape to the 'underside' of the Flip Stage to absorb the transmitted e-beam, eliminating the spurious Al peak, but this is obviously undesirable as that makes STEM imaging impossible.
Has anyone else encountered this with a similar system?
Cheers Roger Ristau Univ of Connecticut
Login Host: 137.99.20.153 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ravi.thakkar369-at-gmail.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Message: Hi Listeners, I am using Tecnai TEM equiped with Oxford EDS. Whenever I open the shutter to start the EDS analysis, it's not getting open, it shows overload, while it was not even opened for the days. Could you guys help me to fix this issue.
Login Host: 129.130.145.59 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both steve.sem-at-icloud.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: steve.sem-at-icloud.com Name: Steve Perry
Title-Subject: [Filtered] Service Engineer for FEI Tecnai
Message: I would like to hear from a service engineer who is capable and available to perform service work on our Tecnai F30.
Located on the West coast USA. Travel and accommodations can be provided if required.
Reply in confidence,
Steve Perry
Login Host: 174.76.17.36 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
For a quick fix - get Beryllium foil of adequate thickness (0.5mm is widely available) and cover areas *behind* the retractable STEM detector exposed to e-beam transmitted through the sample. You could get STEM image with extended detector and EDS + Se image when detector is retracted.
It is possible that once main Al peak is dealt with, some additional stray signal generated by electrons scattered from/through the sample could become detectable - find out where they come from and shield with Be foil.
Be is considered "hazmat," but that is when it is in dust/powder/fume/solution form which can be ingested, inhaled, or absorbed into the body. If you do not drill/sand/machine Be foil but only attach it by conductive vacuum epoxy it should be safe enough to stay there. Lots of Be windows were (and still are) out there installed in SEMs.
If Beryllium is too much of a scare, use Aquadag paint of adequate thickness to cover area behind STEM detector. Best would be to manufacture a shield from some kind of metal, coat it with Aquadag, anneal in vacuum oven -at- 300 degrees, and then mount into SEM chamber.
Best Wishes :) Valery
Valery Ray www.linkedin.com/in/valeryray ============================== PBS&T, MEO Engineering Company 290 Broadway, Suite 298 Methuen, MA 01844, USA Phone: +1-978-305-0479 - leave a message Mobie: +1-978-305-0479 - leave a message E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 2/18/2017 12:44 PM, microscopy.listserver-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: roger.ristau-at-uconn.edu } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } roger.ristau-at-uconn.edu as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: roger.ristau-at-uconn.edu Name: Roger Ristau } } Organization: University of Connecticut } } Title-Subject: [Filtered] FIB STEM EDX } } Message: A question for anyone with FIB-STEM-EDX system: } Our FEI Helios 460F1 has single-sample Flip Stage (the 'turret' type Flip Stage with 180 deg } rotation) and STEM detector for imaging TEM liftouts in situ. When attempting to acquire EDX of the } lifout, mounted on the Flip Stage, using EDAX Octane Plus SDD EDX, the spectrum is overwhelmed by } the Si peak from the STEM detector itself. If the STEM detector is retracted, the Si peak is } replaced by a Al peak from the stage area situated below the STEM position. } } Obviously the EDX detector is 'seeing' the X-rays generated by the e-beam that passes through the } thin liftout sample. However, this problem did not occur in our older Strata FIB with SiLi detector. } I am presuming it may have something to do with the larger collection angle of the SDD. I have } confirmed with EDAX that the detector is installed correctly,the collimator is in position and the } system is operating normally. (There are no problems doing EDX with the sample in the 'bulk stage' } position.) } } My temporary solution is to affix a strip of carbon tape to the 'underside' of the Flip Stage to } absorb the transmitted e-beam, eliminating the spurious Al peak, but this is obviously undesirable } as that makes STEM imaging impossible. } } Has anyone else encountered this with a similar system? } } Cheers } Roger Ristau } Univ of Connecticut } } Login Host: 137.99.20.153 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } }
==============================Original Headers============================== 8, 35 -- From vray-at-partbeamsystech.com Sat Feb 18 13:14:23 2017 8, 35 -- Received: from nm4.bullet.mail.bf1.yahoo.com (nm4.bullet.mail.bf1.yahoo.com [98.139.212.163]) 8, 35 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v1IJENwS001966 8, 35 -- for {microscopy-at-microscopy.com} ; Sat, 18 Feb 2017 13:14:23 -0600 8, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1487445661; bh=1fe6VgbnnVmF55q7McMXYbAnmrIRKYsWcA7y6GWh7+4=; h=Subject:To:References:Cc:From:Date:In-Reply-To:From:Subject; b=uGMeLUE/3KBwbo80hYRR3qU6HJ08YcrUsFREGYXHtJi2HN9cJ/pClbYk9Ye7bDN3QKWoaD5GVytrUtyVcIsVquKnFN7+m18DpgwXvk70Ecen0srVpLo5IT8Ah5OeAZn832Hf2MZBHP/+FZUuO1xk8nbz6gpQc1kJ2iMzf4lz6BUYdNSlsgfGE4BaEeJSOtU/INVMIMWW/DH+lZs0Ia5NCrPs3xQhD0Df2O+VnjIwKZn98b7srRPYL40yuyhafnZpTuU5aZkz4USHmCt2T5TP81lVDmp5t+OpspSLJ0EjMv2IhXbU5DW+oVl9SbrQDUohnSNHp0kgtLz4xeO5ILi/iw== 8, 35 -- Received: from [66.196.81.172] by nm4.bullet.mail.bf1.yahoo.com with NNFMP; 18 Feb 2017 19:21:01 -0000 8, 35 -- Received: from [98.139.211.196] by tm18.bullet.mail.bf1.yahoo.com with NNFMP; 18 Feb 2017 19:21:01 -0000 8, 35 -- Received: from [127.0.0.1] by smtp205.mail.bf1.yahoo.com with NNFMP; 18 Feb 2017 19:21:01 -0000 8, 35 -- X-Yahoo-Newman-Id: 605872.93412.bm-at-smtp205.mail.bf1.yahoo.com 8, 35 -- X-Yahoo-Newman-Property: ymail-3 8, 35 -- X-YMail-OSG: mMcXsfoVM1lZqQV.3CFdk2J.BBWfj1b7tSwuEim4SB.HUwn 8, 35 -- qMBRntpiQ.i4QVKnB_rl_kWQoIFz3ixhsNfMXQ_Hb7JPPbnUcqBoWtxMPQ9A 8, 35 -- _jCDMphgfujnjq8r6XzQA8nXC_PnX2QylJZQjzBJaosCkYytJ0KSB2rc2Pel 8, 35 -- aASk5yVWBbI_rnaGJvrRoMHftzv0dWwXNp9sTo3n4JB.9QLtKC2vZxfVEqNY 8, 35 -- 3rpMKaphhkhTCofLwsYMzrhU.Bujbbh2ztk7ZE6qUcVwuB95_7d81pPrTZJw 8, 35 -- kaHZMJ4BK.X4s6JCjs1RlBbFGT.ta_.8FO8DTxoPBwMg8JQL9cABp1PMEMqx 8, 35 -- UAvBu9pjseMYVvNWMXSZ1ziEFp.hBxkMySqQTfmV7CcaiI.H1HIttdbaRdiS 8, 35 -- 5s0036JhDKpJZHkIte9dsJe_tuia8jmjWmVpBZ4a.LAFKjIvnpPAkc7Y5ntA 8, 35 -- w.ehvEY1jAHS5VB4CVKI8B8gky0uQ1Rs5UL9zK.QFKSUD.kXPb043uX0WtJC 8, 35 -- VlfQvt61_J7IaEmf.upn2m.flVDYhRjzPnGcWNcEtkOuV2rtg.9gWw7xVMVT 8, 35 -- OywvmZY_h 8, 35 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 8, 35 -- Subject: Re: [Microscopy] viaWWW:FIB STEM EDX 8, 35 -- To: microscopy.listserver-at-gmail.com, "Ristau, Roger" {roger.ristau-at-uconn.edu} 8, 35 -- References: {201702181744.v1IHi58Q032489-at-microscopy.com} 8, 35 -- Cc: microscopy-at-microscopy.com 8, 35 -- From: Valery Ray {vray-at-partbeamsystech.com} 8, 35 -- Message-ID: {091f33e1-83af-a20d-62f8-4c4c404ee112-at-partbeamsystech.com} 8, 35 -- Date: Sat, 18 Feb 2017 14:17:50 -0500 8, 35 -- User-Agent: Mozilla/5.0 (Windows NT 10.0; WOW64; rv:45.0) Gecko/20100101 8, 35 -- Thunderbird/45.7.1 8, 35 -- MIME-Version: 1.0 8, 35 -- In-Reply-To: {201702181744.v1IHi58Q032489-at-microscopy.com} 8, 35 -- Content-Type: text/plain; charset=windows-1252; format=flowed 8, 35 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I have a vintage 1980 ARL-SEMQ microprobe, mostly working, that needs a home. This was the deluxe model with all of the bells and whistles available at the time (including CL imaging), and it was updated to the Advanced Microbeam automation system in the 90's. If you have any interest please contact me. I need to get it out of the lab soon to make room for new equipment. I also have nearly an entire second one in spares. If nobody takes it then it all goes to scrap.
Thanks,
Dan
-- **************************************************************************** A man who lies to himself, and believes his own lies, becomes unable to recognize truth, either in himself or in anyone else, and he ends up losing respect for himself and for others. -Fyodor Dostoevsky, novelist (1821-1881) **************************************************************************** Daniel E. Sampson Instrument Engineer A232 Earth and Marine Sciences University of California Santa Cruz, CA 95064 dsampson-at-ucsc.edu (831) 459-4992 office (831) 359-9075 cell (831) 459-3074 FAX ****************************************************
==============================Original Headers============================== 6, 44 -- From dsampson-at-ucsc.edu Tue Feb 21 10:31:38 2017 6, 44 -- Received: from mail-pg0-f53.google.com (mail-pg0-f53.google.com [74.125.83.53]) 6, 44 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v1LGVb7V014903 6, 44 -- for {microscopy-at-microscopy.com} ; Tue, 21 Feb 2017 10:31:38 -0600 6, 44 -- Received: by mail-pg0-f53.google.com with SMTP id z128so769654pgb.0 6, 44 -- for {microscopy-at-microscopy.com} ; Tue, 21 Feb 2017 08:38:26 -0800 (PST) 6, 44 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 44 -- d=ucsc.edu; s=ucsc-google; 6, 44 -- h=to:from:subject:organization:message-id:date:user-agent 6, 44 -- :mime-version:content-transfer-encoding; 6, 44 -- bh=6lK9VyYrxA1Mf5MAwloCO4ZPYHqrphlsn7RjVCfED78=; 6, 44 -- b=cyztaXke7Vg66ZaxVSk8Zwzzgq2xqKhLiKji6Hh9q+S+hi9sMLXnU4PXN0TvGXbiy7 6, 44 -- VFO1HJErDvU3u0vx1lloFNdRkxRuosfD2VDw0pDiu1b1U+LtS7vv+cFx7MEPdaT0dkJN 6, 44 -- xfG5fHZzKa7xciU3SGwXqLk7gmzIlfQuJ89AU= 6, 44 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 44 -- d=1e100.net; s=20161025; 6, 44 -- h=x-gm-message-state:to:from:subject:organization:message-id:date 6, 44 -- :user-agent:mime-version:content-transfer-encoding; 6, 44 -- bh=6lK9VyYrxA1Mf5MAwloCO4ZPYHqrphlsn7RjVCfED78=; 6, 44 -- b=M7xgdqSO1wxEBplhGGKRAtAKi6X3LfgWFszNpj65rc/xOKNMfotNARsDc3DnSvvG9/ 6, 44 -- hjp0uEAd2GMG1XkHZSnzIMSyuPZpUI4v2zx/+8hdE7a6pw39N+XfmR2dXBnYMK97D4X7 6, 44 -- wc48RzgNv40ScJrWOyItuy7X3vST3tvWeYGVJ7tgQ+NxZY3NTx/0VDIyuTUqQ79u7zrc 6, 44 -- Fkk222EO5mwApKVQ1ohpMlEx+9XLJ0mEB/3xAlm+dzTIxNx0Mr2q+cyePFEuTD3eD0Vw 6, 44 -- WQpGQlUUOlGC68dq3p7WtvrIVyahMz/rY45GF37CoTSQzccYBsW7YqtPMTHYTcMCGHYL 6, 44 -- dVag== 6, 44 -- X-Gm-Message-State: AMke39nwkaXIr+TLjhJHMjhygG9Ar+kW4nc4bvgq+hqrdZm/5YqayE27ddU2+7xLcgsWojUN 6, 44 -- X-Received: by 10.99.231.5 with SMTP id b5mr36242466pgi.80.1487695105765; 6, 44 -- Tue, 21 Feb 2017 08:38:25 -0800 (PST) 6, 44 -- Received: from dhcp-25-184.ucsc.edu (dhcp-25-184.ucsc.edu. [128.114.25.184]) 6, 44 -- by smtp.gmail.com with ESMTPSA id a24sm42096951pfh.33.2017.02.21.08.38.23 6, 44 -- for {microscopy-at-microscopy.com} 6, 44 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 6, 44 -- Tue, 21 Feb 2017 08:38:23 -0800 (PST) 6, 44 -- To: microscopy-at-microscopy.com 6, 44 -- From: Daniel Sampson {dsampson-at-ucsc.edu} 6, 44 -- Subject: Free ARL-SEMQ uProbe 6, 44 -- Organization: UC Santa Cruz 6, 44 -- Message-ID: {ab3df56e-1ccc-2e6f-db2d-9358d645b2a6-at-ucsc.edu} 6, 44 -- Date: Tue, 21 Feb 2017 08:38:24 -0800 6, 44 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.12; rv:45.0) 6, 44 -- Gecko/20100101 Thunderbird/45.7.1 6, 44 -- MIME-Version: 1.0 6, 44 -- Content-Type: text/plain; charset=utf-8; format=flowed 6, 44 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ibarke2-at-uwo.ca as well as the Microscopy Listserver ---------------------------------------------------------------------------
A quick question that I hope someone could help me with.
In our daily routine analyses with our Hitachi SU6600 FE-SEM, we are getting a large component of beam drift. As in, the image seems to migrate while doing an analysis, leading to blurry images and maps. When the SEM initially turns out, we can correct for this drift with the stigmators, but eventually, the range for that tops out, and we cannot get crisp images. This occurs with well grounded samples, while doing routine imaging or EDS analyses. We typically analyze geological thin sections, but we coat with carbon and ground with either carbon or silver paint, or copper tape. I originally thought it was due to my grounding, but it used to be fine, and no matter what I do the images still drift.
However, if I put things into variable pressure mode it is fine.
It has been a while since we've done a "bakeout", so I'm just wondering if it could be something related to tip alignment, or if there are particles in the column? What can I do to check the instrument? I just worry there's something going on with the tip. How can I check that?
Thank you in advance for any info and tips! - Ivan
Login Host: 129.100.37.41 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
Status: This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both krueger.eugene-at-mayo.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Message: Hello microscopists and vendors, I was wondering what commercial software packages people are using for 3D modeling of confocal Z-stacks.I can generate beautifully detailed Z-stacks, but would like to be able to make models from the data that I could use in other applications. Specifically, I would like to generate 3D models that I could manipulate and use in movies and presentations. Any information would be appreciated, and vendors are encouraged to reply.
Thank you, -Eugene
Eugene Krueger Sr. Research Tech II Mayo Clinic
Login Host: 129.176.151.18 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
Although it is hard to diagnose remotely, chances are that problem is not with the gun. If drift was related to electron source, then most likely it would be appearing similarly in both HV and variable pressure modes.
There is a good chance that there is some kind of dielectric contamination, particle, or a piece of fiber stuck at the bottom of objective lens. It is getting charged during high-vac operation and deflects/astigmates the beam. When you switch to variable pressure mode then ADAPT is inserted, shielding contamination from the beam, thus preventing the drift. You would need to open specimen chamber and carefully inspect bottom of the column and opening in the polepiece with mirror and a good flashlight, and try to look inside of the polepiece opening. Also take a look on outside of the insertable variable aperture for any traces of contamination or particles. Make sure that in high-vacuum mode ADAPT is fully retracted. Check resistance from polepiece to ground with ohmmeter, however strange this sounds. Also check grounding of your stage - there is some possibility that stage is electrically floating and thus getting charged in High Vac mode.
Best Wishes,
Valery Ray www.linkedin.com/in/valeryray ============================== PBS&T, MEO Engineering Company 290 Broadway, Suite 298 Methuen, MA 01844, USA Phone: +1-978-305-0479 - leave a message Mobie: +1-978-305-0479 - leave a message E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 2/21/2017 10:09 PM, microscopy.listserver-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: ibarke2-at-uwo.ca } } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } ibarke2-at-uwo.ca as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: ibarke2-at-uwo.ca Name: Ivan Barker } } Organization: Western University } } Title-Subject: [Filtered] Hitachi SU6600 FE-SEM Constant Beam Drift? } } Message: Hi all, } } A quick question that I hope someone could help me with. } } In our daily routine analyses with our Hitachi SU6600 FE-SEM, we are getting a large component of } beam drift. As in, the image seems to migrate while doing an analysis, leading to blurry images and } maps. When the SEM initially turns out, we can correct for this drift with the stigmators, but } eventually, the range for that tops out, and we cannot get crisp images. This occurs with well } grounded samples, while doing routine imaging or EDS analyses. We typically analyze geological thin } sections, but we coat with carbon and ground with either carbon or silver paint, or copper tape. I } originally thought it was due to my grounding, but it used to be fine, and no matter what I do the } images still drift. } } However, if I put things into variable pressure mode it is fine. } } It has been a while since we've done a "bakeout", so I'm just wondering if it could be something } related to tip alignment, or if there are particles in the column? What can I do to check the } instrument? I just worry there's something going on with the tip. How can I check that? } } Thank you in advance for any info and tips! } - Ivan } } Login Host: 129.100.37.41 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } }
==============================Original Headers============================== 6, 35 -- From vray-at-partbeamsystech.com Wed Feb 22 00:04:35 2017 6, 35 -- Received: from nm34-vm8.bullet.mail.bf1.yahoo.com (nm34-vm8.bullet.mail.bf1.yahoo.com [72.30.239.6]) 6, 35 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v1M64YGn003077 6, 35 -- for {microscopy-at-microscopy.com} ; Wed, 22 Feb 2017 00:04:35 -0600 6, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1487743885; bh=8YHFI87oO9r/3xrZjLxAChnbdfsxf5hkEx7i5zvfzuU=; h=Subject:To:References:From:Cc:Date:In-Reply-To:From:Subject; b=Js44PENJrGNwgqdSEb4Guy/mahduy7t7rLskpFFtw7kBjSQL+wv8mX2l60zm0ZgJXjkA8zINUAJbxstg116urJKI0nnh51/o7Pb9nExhJixZ7eSC8yiR+qI2UlH7zjCKhFEdaBVk1KCdQkA9EiHl8ePLGjOLGQ42y39YnLEh7H2u6G+ia3f0b2ifwJ3wJcitQSY7xjjzAducuHXBbNydbBJSykpuaL9NeuuARS0UY+bUx68gvGuc0hzkiIfWHKBMwWv8+bypceoYwW0H1MTr34uYcegq77XKnC8F+z0oiU9hZpDoSqQpUwzMpFaAa8CoUxamGUjBdZ2W9qMnuA84Mg== 6, 35 -- Received: from [98.139.170.178] by nm34.bullet.mail.bf1.yahoo.com with NNFMP; 22 Feb 2017 06:11:25 -0000 6, 35 -- Received: from [68.142.230.72] by tm21.bullet.mail.bf1.yahoo.com with NNFMP; 22 Feb 2017 06:11:25 -0000 6, 35 -- Received: from [127.0.0.1] by smtp229.mail.bf1.yahoo.com with NNFMP; 22 Feb 2017 06:11:25 -0000 6, 35 -- X-Yahoo-Newman-Id: 550371.29157.bm-at-smtp229.mail.bf1.yahoo.com 6, 35 -- X-Yahoo-Newman-Property: ymail-3 6, 35 -- X-YMail-OSG: vw.SJ_IVM1kcKKLBkISsO0z.5Zm6NPo80ORj6zLoy5fpANG 6, 35 -- 4ZHCLlHhEEEPvALqIQWdVVd9znpNH.MGfganbd.lkrzpxe04sxNkGMJfLBCk 6, 35 -- PU3QA3Bs9QAcIQyUhv.0IsuK3PnQnq2Ith_dvGUURtGHWulnDMDEV1QnMGap 6, 35 -- 5EmkYdmWz8XcKdtdoxlTQnrK897r1c5lcTfmQqHIORPwH4hFK.qF3EFihNKS 6, 35 -- SDwPKTxm3zvbaMkIJbuUAV.1L2HTT4dHZ8gKvf9O2_rmTpdoPMo.ssvq4.5k 6, 35 -- 4PnsQVFWTzpJhuSPHp2balPGjqWO7XRl0ZLSPfGI9_28OvT3kalA3yGmmwOg 6, 35 -- U1YzHLmHdua.PPSWdmW9aGBgF0e7ffABesIWraqvk5341sYRmUcTvlGBEgXl 6, 35 -- x1yqivW9O3MZAOSH.BROqbMkeFzN1_P6eg1jecyOtx2Jlnb7SkAosC.7Ypmh 6, 35 -- kMHbwy3NAenSBwMOvbIaugrDPk1qok2Fa3wtxog0m2f8vRTiKUCh7GrMWtTh 6, 35 -- HLF4UcTk5cHULB7bmF1pqPWxbF2Dd05CvL0HMRYrwILf_KgvpGA4ChvX5yrw 6, 35 -- vEidBUS1S 6, 35 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 6, 35 -- Subject: Re: [Microscopy] viaWWW:Hitachi SU6600 FE-SEM Constant Beam Drift? 6, 35 -- To: ibarke2-at-uwo.ca 6, 35 -- References: {201702220309.v1M396IE022755-at-microscopy.com} 6, 35 -- From: Valery Ray {vray-at-partbeamsystech.com} 6, 35 -- Cc: microscopy-at-microscopy.com 6, 35 -- Message-ID: {c23ddbb8-f915-991a-0b22-df5d95f57b3f-at-partbeamsystech.com} 6, 35 -- Date: Wed, 22 Feb 2017 01:08:08 -0500 6, 35 -- User-Agent: Mozilla/5.0 (Windows NT 10.0; WOW64; rv:45.0) Gecko/20100101 6, 35 -- Thunderbird/45.7.1 6, 35 -- MIME-Version: 1.0 6, 35 -- In-Reply-To: {201702220309.v1M396IE022755-at-microscopy.com} 6, 35 -- Content-Type: text/plain; charset=windows-1252; format=flowed 6, 35 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
We had a similar problem with a Hitachi S-2460N many years ago. I don't know how it compares to the 6600.
We had let the PM schedule lapse and got similar drifting. The engineer came in and cleaned out the liner tube, as I recall. It had built up a deposit on one side that took on a charge in hivac mode. Once it was cleaned, things were back to normal with no drift in hivac or lovac mode.
Warren Straszheim Materials Analysis and Research Lab Iowa State University
-----Original Message----- X-from: vray-at-partbeamsystech.com [mailto:vray-at-partbeamsystech.com] Sent: Wednesday, February 22, 2017 12:06 AM To: Straszheim, Warren E [BIOTC]
Mildred Dresselhaus, a professor emerita at the Massachusetts Institute of Technology whose research into the fundamental properties of carbon helped transform it into the superstar of modern materials science and the nanotechnology industry, died on Monday in Cambridge, Mass. She was 86.
A. John Mardinly, Ph.D., P.E. Retired Principal Materials Nanoanalysis Engineer
==============================Original Headers============================== 11, 90 -- From John.Mardinly-at-asu.edu Fri Feb 24 10:21:40 2017 11, 90 -- Received: from bcnetw1.asu.edu (bcnetw1.asu.edu [149.169.2.71]) 11, 90 -- by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v1OGLdja020966 11, 90 -- for {Microscopy-at-Microscopy.com} ; Fri, 24 Feb 2017 10:21:40 -0600 11, 90 -- X-ASG-Debug-ID: 1487953718-0d12c74e4505090001-FOsErg 11, 90 -- Received: from ppnetw4.asu.edu (ppnetw4.asu.edu [10.118.52.71]) by bcnetw1.asu.edu with ESMTP id 9kvOWkAY2tmCZpFV (version=TLSv1.2 cipher=ECDHE-RSA-AES256-GCM-SHA384 bits=256 verify=NO) for {Microscopy-at-Microscopy.com} ; Fri, 24 Feb 2017 09:28:38 -0700 (MST) 11, 90 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu 11, 90 -- Received: from pps.filterd (ppnetw4.asu.edu [127.0.0.1]) 11, 90 -- by ppnetw4.asu.edu (8.16.0.17/8.16.0.17) with SMTP id v1OGQEEO039223 11, 90 -- for {Microscopy-at-Microscopy.com} ; Fri, 24 Feb 2017 09:28:38 -0700 11, 90 -- Received: from exhubw01.asurite.ad.asu.edu (exnswvpx-10-118-52-65.inre.asu.edu [10.118.52.65]) 11, 90 -- by ppnetw4.asu.edu with ESMTP id 28t46ghed9-1 11, 90 -- (version=TLSv1 cipher=ECDHE-RSA-AES256-SHA bits=256 verify=NOT) 11, 90 -- for {Microscopy-at-Microscopy.com} ; Fri, 24 Feb 2017 09:28:37 -0700 11, 90 -- Received: from NAM02-SN1-obe.outbound.protection.outlook.com (129.219.4.240) 11, 90 -- by exhubgslb.asu.edu (129.219.4.199) with Microsoft SMTP Server (TLS) id 11, 90 -- 14.3.266.1; Fri, 24 Feb 2017 09:28:37 -0700 11, 90 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 11, 90 -- d=arizonastateu.onmicrosoft.com; s=selector1-asu-edu; 11, 90 -- h=From:Date:Subject:Message-ID:Content-Type:MIME-Version; 11, 90 -- bh=4s5Qspi0kZ5RLDsNXGllsjqNXs1qV/QwzNWzClfPx74=; 11, 90 -- b=QejntLN/DBo+PwbMKOofO28LWjpI7lRmCQJ8PdKKXXO2BWYl0SQ2jm7G0Zfv7XU8ZM2/b/Eh5NVPUDeUkOfgMDq++GBjn89csD9x8mMUbzgUBPgL+efZNYXRJVpPWN1CcSuye2rjPDhAFmruRT2h+KMeiplPCdlBacXz5H/QiYI= 11, 90 -- X-Barracuda-Effective-Source-IP: exhubw01.asurite.ad.asu.edu[129.219.4.199] 11, 90 -- X-Barracuda-Apparent-Source-IP: 129.219.4.199 11, 90 -- Received: from CY4PR06MB2904.namprd06.prod.outlook.com (10.175.118.144) by 11, 90 -- CY4PR06MB2902.namprd06.prod.outlook.com (10.175.118.142) with Microsoft SMTP 11, 90 -- Server (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_256_CBC_SHA384_P384) id 11, 90 -- 15.1.919.13; Fri, 24 Feb 2017 16:28:36 +0000 11, 90 -- Received: from CY4PR06MB2904.namprd06.prod.outlook.com ([10.175.118.144]) by 11, 90 -- CY4PR06MB2904.namprd06.prod.outlook.com ([10.175.118.144]) with mapi id 11, 90 -- 15.01.0919.018; Fri, 24 Feb 2017 16:28:36 +0000 11, 90 -- From: John Mardinly {John.Mardinly-at-asu.edu} 11, 90 -- To: MSA {Microscopy-at-Microscopy.com} 11, 90 -- Subject: Mildred Dresselhaus died on Monday in Cambridge, Mass. She was 86. 11, 90 -- Thread-Topic: Mildred Dresselhaus died on Monday in Cambridge, Mass. She was 11, 90 -- 86. 11, 90 -- X-ASG-Orig-Subj: Mildred Dresselhaus died on Monday in Cambridge, Mass. She was 86. 11, 90 -- Thread-Index: AQHSjrsMJwOv8lotyE+/YeIx5xOrbQ== 11, 90 -- Date: Fri, 24 Feb 2017 16:28:36 +0000 11, 90 -- Message-ID: {309327FB-3B56-44CD-B87D-89F42B395290-at-asu.edu} 11, 90 -- Accept-Language: en-US 11, 90 -- Content-Language: en-US 11, 90 -- X-MS-Has-Attach: 11, 90 -- X-MS-TNEF-Correlator: 11, 90 -- x-ms-exchange-messagesentrepresentingtype: 1 11, 90 -- x-originating-ip: [68.225.210.79] 11, 90 -- x-ms-office365-filtering-correlation-id: c4892089-c9c4-427d-0760-08d45cd22ece 11, 90 -- x-microsoft-antispam: UriScan:;BCL:0;PCL:0;RULEID:(22001);SRVR:CY4PR06MB2902; 11, 90 -- x-microsoft-exchange-diagnostics: 1;CY4PR06MB2902;7:Cax7yK2cMTO7koYcjt7oSZO3ts/ZHm2qu15C2A5KNp3OwYBbvyZvakO9gPuvutw/UK6aUESjw+DOM0WFmrO6I/559CLhNnWjXshQeVhdyszcGJmSMh8JInIsQ3SRiqpPuSS434cl15sMv2+jj7jSCfEyssdPfzFoWFcMbzeaYpiU5OYd3dxlW4CXh6VAH3PkMv9drdDtmHf7Hcge1D2Bw3x9aPSXtQCQC4tbyqQegsdcmzxlwCTHK6Z5pBN7LAtFdua3vOgFUOrn16kyxwxdJZxgNF16SY4JtqCkAsEz0tv/pMtIa6g6Go7aNfWBkMXAIu1Tk0KytACcF1szo6rV9A== 11, 90 -- x-microsoft-antispam-prvs: {CY4PR06MB29023119829F072C7CB31CFEE2520-at-CY4PR06MB2902.namprd06.prod.outlook.com} 11, 90 -- x-exchange-antispam-report-test: UriScan:; 11, 90 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:(6040375)(601004)(2401047)(8121501046)(5005006)(3002001)(10201501046)(6041248)(20161123555025)(20161123564025)(20161123562025)(20161123560025)(20161123558025)(6072148);SRVR:CY4PR06MB2902;BCL:0;PCL:0;RULEID:;SRVR:CY4PR06MB2902; 11, 90 -- x-forefront-prvs: 0228DDDDD7 11, 90 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10009020)(6009001)(7916002)(39450400003)(199003)(189002)(81166006)(2906002)(86362001)(3660700001)(82746002)(6116002)(110136004)(68736007)(81156014)(33656002)(3280700002)(8676002)(105586002)(88552002)(106116001)(83716003)(66066001)(106356001)(38730400002)(53936002)(7736002)(189998001)(50986999)(54356999)(6512007)(77096006)(99286003)(6436002)(6916009)(6506006)(97736004)(450100001)(2900100001)(305945005)(36756003)(8936002)(92566002)(101416001)(25786008)(102836003)(5660300001)(3846002)(122556002)(6486002)(75432002)(104396002);DIR:OUT;SFP:1101;SCL:1;SRVR:CY4PR06MB2902;H:CY4PR06MB2904.namprd06.prod.outlook.com;FPR:;SPF:None;PTR:InfoNoRecords;A:1;MX:1;LANG:en; 11, 90 -- received-spf: None (protection.outlook.com: asu.edu does not designate 11, 90 -- permitted sender hosts) 11, 90 -- spamdiagnosticoutput: 1:99 11, 90 -- spamdiagnosticmetadata: NSPM 11, 90 -- Content-Type: text/plain; charset="us-ascii" 11, 90 -- Content-ID: {1B99959508F51A4E9026544DB7C652E7-at-namprd06.prod.outlook.com} 11, 90 -- MIME-Version: 1.0 11, 90 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 24 Feb 2017 16:28:36.2763 11, 90 -- (UTC) 11, 90 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted 11, 90 -- X-MS-Exchange-CrossTenant-id: 41f88ecb-ca63-404d-97dd-ab0a169fd138 11, 90 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: CY4PR06MB2902 11, 90 -- X-OriginatorOrg: asu.edu 11, 90 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:,, definitions=2017-02-24_11:,, 11, 90 -- signatures=0 11, 90 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 suspectscore=0 11, 90 -- malwarescore=0 phishscore=0 adultscore=0 bulkscore=0 classifier=spam 11, 90 -- adjust=0 reason=mlx scancount=1 engine=8.0.1-1612050000 11, 90 -- definitions=main-1702240160 11, 90 -- X-Barracuda-Connect: ppnetw4.asu.edu[10.118.52.71] 11, 90 -- X-Barracuda-Start-Time: 1487953718 11, 90 -- X-Barracuda-Encrypted: ECDHE-RSA-AES256-GCM-SHA384 11, 90 -- X-Barracuda-URL: https://149.169.2.71:443/cgi-mod/mark.cgi 11, 90 -- X-Barracuda-Scan-Msg-Size: 391 11, 90 -- X-Virus-Scanned: by bsmtpd at asu.edu 11, 90 -- X-Barracuda-BRTS-Status: 1 11, 90 -- X-Barracuda-Spam-Score: 0.02 11, 90 -- X-Barracuda-Spam-Status: No, SCORE=0.02 using global scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=5.0 tests=THREAD_INDEX, THREAD_TOPIC 11, 90 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.36773 11, 90 -- Rule breakdown below 11, 90 -- pts rule name description 11, 90 -- ---- ---------------------- -------------------------------------------------- 11, 90 -- 0.01 THREAD_INDEX thread-index: AcO7Y8iR61tzADqsRmmc5wNiFHEOig== 11, 90 -- 0.01 THREAD_TOPIC Thread-Topic: ...(Japanese Subject)... 11, 90 -- Content-Transfer-Encoding: 8bit 11, 90 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v1OGLdja020966 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both vakimler-at-oakland.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Organization: Oakland University Eye Research Institute
Title-Subject: [Filtered] Carbon Grains on Formvar Grids or Carbon Film
Message: Sometimes it is very difficult to tell between what is sample and what is carbon grain (either grids coated with compressed carbon) or the carbon on Formvar grids. Can anyone shed light on this when one does protein analysis?
We are working at 100kV and 140Kx mag.
Thanks!
Login Host: 141.210.132.220 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
Microscopy-at-Microscopy.com
============================================
==============================Original Headers============================== 15, 52 -- From microscopy.listserver-at-gmail.com Sat Feb 25 09:49:55 2017 15, 52 -- Received: from mail-it0-f54.google.com (mail-it0-f54.google.com [209.85.214.54]) 15, 52 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v1PFntc8008156 15, 52 -- for {microscopy-at-microscopy.com} ; Sat, 25 Feb 2017 09:49:55 -0600 15, 52 -- Received: by mail-it0-f54.google.com with SMTP id y135so46440367itc.1 15, 52 -- for {microscopy-at-microscopy.com} ; Sat, 25 Feb 2017 07:56:58 -0800 (PST) 15, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 15, 52 -- d=gmail.com; s=20161025; 15, 52 -- h=from:subject:references:reply-to:to:message-id:date:user-agent 15, 52 -- :mime-version:in-reply-to:content-transfer-encoding; 15, 52 -- bh=kBsZxknsLUuSnWdVLT9i7MxTRzmgGk2voPyx5vzdmbg=; 15, 52 -- b=lenWrIpnBlMFh+UtZ7JijNr4Nz019cAZjIaXEnKtbUvJNerx3BA47LTz+r8UXKLEom 15, 52 -- /WsATehO7V+hiKIo15FHkY6qVtoJOl+CfQdGrUoxOceoXcqaXyIT2lMtUCQjVeGqTfmM 15, 52 -- jYlOUNPzYuXN8eYHW2dsxNbIWdhPBn4KAx2fAGbWDAXRRKppsTsR0Vp5aeKr09Km+ppy 15, 52 -- ItC8TjILvwqA5TeJzFXFJ/P5CSXooojCdndi1Q9rBeoXgTuyDCv0Dzyd/kxWTg/MxdUx 15, 52 -- 9+K/OYSg9mdo02J3MX9+vNuB8IL9VFyFjTz6HlakTy3bpZ5laNbb8OIH577Dqbg9TjSn 15, 52 -- Dipg== 15, 52 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 15, 52 -- d=1e100.net; s=20161025; 15, 52 -- h=x-gm-message-state:from:subject:references:reply-to:to:message-id 15, 52 -- :date:user-agent:mime-version:in-reply-to:content-transfer-encoding; 15, 52 -- bh=kBsZxknsLUuSnWdVLT9i7MxTRzmgGk2voPyx5vzdmbg=; 15, 52 -- b=AQYiMGJvMULy6xadD3SpRiaQwpQtPPzhmVySgB2CK42qh54kFivGSa4q5eHrclN9Aq 15, 52 -- FSAQuBAQNET5ND6F3HP2XlctBtfWSEoubnjHiIwvb04fCvRKstava5kswNFzb492QP4l 15, 52 -- 1FILttj6EejUSitQ8pPFQWBo6kP0xF+aRa1h+2Fnilwhs5H76VIpsq4LtIEsgoIvMxR6 15, 52 -- e3hk/qT7BQFyhOVb+PvVM287Vu0ck0t7t3e5KyqR/k4K8E3U/jcH7G99GZBxyqbhte4G 15, 52 -- gHmMQhVqC6LC9gNUR4hcU+bbvABVW3svOT+zN9EdLVbqo9Ahb/JfjKHgs7fkK+/pguj1 15, 52 -- bm+g== 15, 52 -- X-Gm-Message-State: AMke39ko9nvhqUzTeampEip+aGCJoPSDN72TTruYPXMZYYpTPsgCJF0EivHcsf8Rf3nXUw== 15, 52 -- X-Received: by 10.36.2.65 with SMTP id 62mr7592245itu.83.1488038218093; 15, 52 -- Sat, 25 Feb 2017 07:56:58 -0800 (PST) 15, 52 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:9ce4:1fac:9eca:1af9]) 15, 52 -- by smtp.googlemail.com with ESMTPSA id y42sm4517442ioi.21.2017.02.25.07.56.57 15, 52 -- for {microscopy-at-microscopy.com} 15, 52 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 15, 52 -- Sat, 25 Feb 2017 07:56:57 -0800 (PST) 15, 52 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 15, 52 -- X-Google-Original-From: MicroscopyListserver-NoReply 15, 52 -- {microscopylistserver-noreply-at-microscopy.com} 15, 52 -- Subject: viaWWW:Carbon Grains on Formvar Grids or Carbon Film 15, 52 -- References: {201702242122.v1OLMN5g024836-at-microscopy.com} 15, 52 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 15, 52 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 15, 52 -- X-Forwarded-Message-Id: {201702242122.v1OLMN5g024836-at-microscopy.com} 15, 52 -- Message-ID: {7f734a9f-5008-db09-4794-ff2d3c55cf54-at-microscopy.com} 15, 52 -- Date: Sat, 25 Feb 2017 09:56:56 -0600 15, 52 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:45.0) 15, 52 -- Gecko/20100101 Thunderbird/45.7.1 15, 52 -- MIME-Version: 1.0 15, 52 -- In-Reply-To: {201702242122.v1OLMN5g024836-at-microscopy.com} 15, 52 -- Content-Type: text/plain; charset=windows-1252; format=flowed 15, 52 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
carbon films come in different grades one of which is 'ultra-thin' and has areas down to 3nm thick according to the publicity in catalogs. I've never done protein analysis, but you need a supporting substrate and one that is amorphous, so try and look in the holes since they may be covered with thin carbon, the thinner the better.
I have bought some silicon nitride supports that are thin and they can be plasma cleaned between uses, but they are a bit pricey.
Good luck Rob
-- Robert Keyse, DPhil Research Scientist Lehigh University
5, East Packer Avenue, Whitaker Laboratory Bethlehem, PA 18015-3194
Office (610)758-3465 Fax (610)758-4244
==============================Original Headers============================== 8, 33 -- From rok210-at-lehigh.edu Mon Feb 27 10:23:12 2017 8, 33 -- Received: from rain4.cc.lehigh.edu (rain4.cc.lehigh.edu [128.180.3.218]) 8, 33 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v1RGNCpR027453 8, 33 -- for {Microscopy-at-Microscopy.com} ; Mon, 27 Feb 2017 10:23:12 -0600 8, 33 -- Received: from [127.0.0.1] (rok210pc.mat.lehigh.edu [128.180.54.183]) 8, 33 -- (using TLSv1.2 with cipher ECDHE-RSA-AES128-GCM-SHA256 (128/128 bits)) 8, 33 -- (No client certificate requested) 8, 33 -- (Authenticated sender: rok210) 8, 33 -- by rain4.cc.lehigh.edu (Postfix) with ESMTPSA id 158B850393EF; 8, 33 -- Mon, 27 Feb 2017 11:30:21 -0500 (EST) 8, 33 -- To: Microscopy-at-Microscopy.com 8, 33 -- Cc: vakimler-at-oakland.edu 8, 33 -- From: Robert Keyse {rok210-at-lehigh.edu} 8, 33 -- Subject: Re: [Microscopy] viaWWW:Carbon Grains on Formvar Grids or Carbon Film 8, 33 -- Message-ID: {b30b53c4-17e7-5733-752e-23875ea0088d-at-lehigh.edu} 8, 33 -- Date: Mon, 27 Feb 2017 11:30:21 -0500 8, 33 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:45.0) Gecko/20100101 8, 33 -- Thunderbird/45.7.1 8, 33 -- MIME-Version: 1.0 8, 33 -- Content-Type: text/plain; charset=utf-8; format=flowed 8, 33 -- Content-Transfer-Encoding: 7bit 8, 33 -- X-Antivirus: avast! (VPS 170227-0, 02/27/2017), Outbound message 8, 33 -- X-Antivirus-Status: Clean 8, 33 -- X-Virus-Scanned: clamav-milter 0.99.2 at rain4.CC.lehigh.EDU 8, 33 -- X-Virus-Status: Clean 8, 33 -- X-Spam-Status: No, score=-4.0 required=5.0 tests=ALL_TRUSTED,LU_FORGED_SENDER, 8, 33 -- RP_MATCHES_RCVD autolearn=disabled version=3.4.1 8, 33 -- X-Spam-Report: 8, 33 -- * -5.0 ALL_TRUSTED Passed through trusted hosts only via SMTP 8, 33 -- * -0.0 RP_MATCHES_RCVD Envelope sender domain matches handover relay domain 8, 33 -- * 1.0 LU_FORGED_SENDER No description available. 8, 33 -- X-Spam-Checker-Version: SpamAssassin 3.4.1 (2015-04-28) on 8, 33 -- freddie.cc.lehigh.edu ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both allan.mitchell-at-otago.ac.nz as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: allan.mitchell-at-otago.ac.nz Name: Allan Mitchell
Organization: Otago centre for Electron Microscopy, University of Otago
Title-Subject: [Filtered] Ultramicrotomes and gridstainers
Message: We are in the process of trying to obtain funding to replace one of our ultramicrotomes (Ultracut E) and our aged LKB Ultrostainer. We are looking at - RMC Boeckeler ultramicrotme PT-PCZ and the RMC Boeckeler QG-3100 TEM Stainer, or
- Leica Ultracut EM UC7 ultramicrotome and Leica EM AC20 grid-stainer.
I would be keen to hear of peoples experiences with any of these instruments, positives /negatives.
Many thanks
Allan
Login Host: 139.80.40.139 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both duraine-at-bcm.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: duraine-at-bcm.edu Name: Lita Duraine
Organization: Howard Hughes Medical Institute
Title-Subject: [Filtered] Re: viaWWW:Ultramicrotomes and gridstainers
Message: Hi Allan,
I totally support the Leica Ultracut EM UC7 microtome. We bought one in 2013 and it has worked efficiently ever since. It is very user friendly and the program settings are easy to manage. Hardly any break-in time. The thickness settings stay consistent. You also have a wide latitude for cutting speeds. There are several light settings especially helpful when I am hand trimming a block and then want to go immediately to cutting thins. This instrument is so accurate that it can cut silver sections effortlessly even on the 11th floor of our building! We loved it so much that we bought a second one two years later when we expanded our lab. Customer service is another reason why I use Leica. We had a small static shock occurring in the new room where we put the microtome. Not sure what was causing it, I called Leica. Immediately, they sent an engineer to our location in Houston and checked everything on the UC7. Several issues were found within the room actually, and everything turned out great. The UC7 is a workhorse, and never skips a beat. I highly recommend the Leica UC7 Ultramicrotome.
Lita Duraine Certified Electron Microscopist Howard Hughes Medical Institute Houston, TX 77030
Login Host: 128.249.96.34 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both stacie-at-ems-secure.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] The Materials Ultramicrotomy Workshop
Message: Three days of hands-on training for technicians, researchers, and students who want to apply a faster and cleaner preparation method that provides samples with uniform thickness, no embedded contamination, and is cheaper than a FIB.
Details: Wednesday - Friday April 12-14, 2017 8:00 a.m. - 4:30 p.m. EMS Microscopy Academy Hatfield, Pennsylvania, USA
Instructors: Helmut Gnaegi, Diatome Ltd., Switzerland Robert Ranner, Leica, Austria Class size is limited! Register now! For more information, visit: http://www.emsdiasum.com/microscopy/academy/courses/materials_ultramicrotomy.aspx
Login Host: 73.165.127.32 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
Inter/Micro 2017 69th Annual International Microscopy Conference June 12 - 16, 2017 at McCrone Research Institute, 2820 S. Michigan Avenue, Chicago, IL 60616
Call for Papers: Speaker Presentations
June 12-14: McCrone Research Institute cordially invites you to give a presentation of your microscopy research at the 69th annual Inter/Micro conference in Chicago. Join professional and amateur microscopists from around the world as they present new research on techniques and instrumentation, environmental and industrial microscopy, and chemical and forensic microscopy. Speakers receive at $50 registration discount. The abstract submission deadline is March 17, 2017. View abstract submission guidelines at:
Tony .......................... Andrew Anthony "Tony" Havics, CIH, PE Environmental, Health & Safety, Microscopy, Materials Science & Forensic Engineering pH2, LLC 5250 E US Highway 36, Suite 830 Avon, IN 46123 (317) 718-7020 Office (317) 718-7038 Fax (317) 409-3238 Cell aahavics-at-pH2LLC.com www.ph2LLC.com
This message is from pH2. This message and any attachments may contain legally privileged or confidential information, and are intended only for the individual or entity identified above as the addressee. If you are not the addressee, or if this message has been addressed to you in error, you are not authorized to read, copy, or distribute this message and any attachments, and we ask that you please delete this message and attachments (including all copies) and notify the sender by return e-mail or by phone at 317-718-7020. Delivery of this message and any attachments to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or a privilege. All personal messages express views only of the sender, which are not to be attributed to pH2 and may not be copied or distributed without this statement.
==============================Original Headers============================== 11, 27 -- From ph2-at-sprynet.com Thu Mar 2 18:55:26 2017 11, 27 -- Received: from elasmtp-banded.atl.sa.earthlink.net (elasmtp-banded.atl.sa.earthlink.net [209.86.89.70]) 11, 27 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v230tQVJ011830 11, 27 -- for {microscopy-at-microscopy.com} ; Thu, 2 Mar 2017 18:55:26 -0600 11, 27 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 11, 27 -- s=dk20050327; d=sprynet.com; 11, 27 -- b=aTxKaNsXrIxV6gL330jmUFtNcBYxHpn6hMi87Cjw5KSlvfffMaSkTd00fq/Ek4wc; 11, 27 -- h=Received:From:To:Subject:Date:Message-ID:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Mailer:Thread-Index:Content-Language:X-ELNK-Trace:X-Originating-IP; 11, 27 -- Received: from [68.58.112.163] (helo=LAPTOPM9E4LFT9) 11, 27 -- by elasmtp-banded.atl.sa.earthlink.net with esmtpa (Exim 4.67) 11, 27 -- (envelope-from {ph2-at-sprynet.com} ) 11, 27 -- id 1cjbcJ-0002bN-HJ 11, 27 -- for microscopy-at-microscopy.com; Thu, 02 Mar 2017 20:02:43 -0500 11, 27 -- From: "Tony Havics, CIH, PE" {ph2-at-sprynet.com} 11, 27 -- To: "Microscopy Listserve" {microscopy-at-microscopy.com} 11, 27 -- Subject: Inter/Micro 2017, Chicago, IL June 12-16, 2017 11, 27 -- Date: Thu, 2 Mar 2017 20:02:43 -0500 11, 27 -- Message-ID: {011101d293b9$dda07900$98e16b00$-at-sprynet.com} 11, 27 -- MIME-Version: 1.0 11, 27 -- Content-Type: text/plain; 11, 27 -- charset="us-ascii" 11, 27 -- Content-Transfer-Encoding: 7bit 11, 27 -- X-Mailer: Microsoft Outlook 16.0 11, 27 -- Thread-Index: AdKTtcl/BYgFGZW9SOKLZNnq/Or/nw== 11, 27 -- Content-Language: en-us 11, 27 -- X-ELNK-Trace: 6888e50b2be9b4fee5331016acda17f9f98cdfe4d7154e7223dd534c76639a3a350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c 11, 27 -- X-Originating-IP: 68.58.112.163 ==============================End of - Headers==============================
Our Hitachi S-2700 SEM will no longer produce an image. We can only see a series of vertical lines of varying intensity with both secondary electron and backscatter detectors. We do have an electron beam - there are changes in intensity of the vertical lines during adjustment of filament current, beam tilt, beam horizon, changes in the lines if we move the specimen. We do lose the image if the HV is turned off on the secondary electron detector. A very similar pattern is seen with the backscatter detector, so it does not appear to be a detector issue.
We have exchanged circuit boards and even the entire column (!) from a second identical scope used for parts. A commercial technician was unable to correct the problem. His best guess is that one or more of the lenses are not functioning.
Any suggestions as to what the problem is? I can send images off-line.
Jeffrey Thompson, Ph.D. Professor of Biology California State University 5500 University Pkwy San Bernardino, CA 92407 909-537-5315
==============================Original Headers============================== 7, 49 -- From jthompso-at-csusb.edu Thu Mar 2 19:06:25 2017 7, 49 -- Received: from NAM03-BY2-obe.outbound.protection.outlook.com (mail-by2nam03on0075.outbound.protection.outlook.com [104.47.42.75]) 7, 49 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v2316P03021393 7, 49 -- for {Microscopy-at-microscopy.com} ; Thu, 2 Mar 2017 19:06:25 -0600 7, 49 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=csusb.edu; 7, 49 -- s=selector1; h=From:Date:Subject:Message-ID:Content-Type:MIME-Version; 7, 49 -- bh=2DXHl+sdv7lZfoI1ZlAV7dldcoThOfcItUM8cr4e4Yg=; 7, 49 -- b=ItH5GzlWhXr5lMs9/qNc4uSnMCV7/fCa5nYZJSY1jKHojRCLtbNETh/vXuYiUmC/yoCWF1KbpA4Cm5gR5FhCj85Sn0OlkEwjp5nNW2gBMjGiqbU+42e0tzNtfK9lgXGFV+a/wyCFSdayxhfFkLeSEwfcwLizCNu58xUFMvn4Ikw= 7, 49 -- Received: from CY4PR08MB2869.namprd08.prod.outlook.com (10.173.59.147) by 7, 49 -- CY4PR08MB2871.namprd08.prod.outlook.com (10.173.59.149) with Microsoft SMTP 7, 49 -- Server (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_256_CBC_SHA384_P384) id 7, 49 -- 15.1.933.12; Fri, 3 Mar 2017 01:13:47 +0000 7, 49 -- Received: from CY4PR08MB2869.namprd08.prod.outlook.com ([10.173.59.147]) by 7, 49 -- CY4PR08MB2869.namprd08.prod.outlook.com ([10.173.59.147]) with mapi id 7, 49 -- 15.01.0933.020; Fri, 3 Mar 2017 01:13:47 +0000 7, 49 -- From: Jeffrey Thompson {jthompso-at-csusb.edu} 7, 49 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 7, 49 -- Subject: SEM Need help in understanding loss of image generation 7, 49 -- Thread-Topic: SEM Need help in understanding loss of image generation 7, 49 -- Thread-Index: AdKTusYGL9em7MiDQpOEkIYERiIIyQ== 7, 49 -- Date: Fri, 3 Mar 2017 01:13:47 +0000 7, 49 -- Message-ID: {CY4PR08MB286955557359A0BF5875540FCC2B0-at-CY4PR08MB2869.namprd08.prod.outlook.com} 7, 49 -- Accept-Language: en-US 7, 49 -- Content-Language: en-US 7, 49 -- X-MS-Has-Attach: 7, 49 -- X-MS-TNEF-Correlator: 7, 49 -- authentication-results: microscopy.com; dkim=none (message not signed) 7, 49 -- header.d=none;microscopy.com; dmarc=none action=none header.from=csusb.edu; 7, 49 -- x-originating-ip: [2607:f380:a61:142:5583:ac34:b43d:3ef6] 7, 49 -- x-ms-office365-filtering-correlation-id: 26778956-7cca-48ee-dd69-08d461d28b35 7, 49 -- x-microsoft-antispam: UriScan:;BCL:0;PCL:0;RULEID:(22001);SRVR:CY4PR08MB2871; 7, 49 -- x-microsoft-exchange-diagnostics: 1;CY4PR08MB2871;7:rHS23JLozCsvFvo6phag6YemA56+clfV4rUPzDzQFY3NAqQSpVYtmBAOD5jSvxl8S/e2ourHvZApghyjoYRRY8Yq6cBuVKOQ6o8jHQ0tvKSqR9zGPKxCpmcmEGYSYLowGN9xDM0fS8yoHnpVwoH1ByQS0e1K442YN53nI3y/jv2Ur6xkOObcQmAUv+hhLNw0KRFWCxWLKM/zP0uQSui2YG8SOyekfRXzW33lZc1slb0y6obbxDehbW7027o/+FI+Tvrxbir+/dYbH6WHOi/8V+2SQsGEbPhRnlyktrO/TRTq4SNIAw3eWz62GreW3q3DMITuu7GTDqB9TAatF2jBLA==;20:FBEKbZT5i0wXScOUNyIugqVbQUjmHgFIfXKT2lgYME4k+z99AAECHORHR9o907/ivCzudIgqpsG6MDtbIXEhVqR5A7vJJZKpJogcHTCT4DokeVZLB1VDVrvyTTjAOj/BWXIpZwT4HKufvDYPtJMlQkpoyNXEHMcSu5q76tKAgjs= 7, 49 -- x-microsoft-antispam-prvs: {CY4PR08MB2871632F020A7C5B5B32C212CC2B0-at-CY4PR08MB2871.namprd08.prod.outlook.com} 7, 49 -- x-exchange-antispam-report-test: UriScan:; 7, 49 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:(6040375)(601004)(2401047)(8121501046)(5005006)(3002001)(10201501046)(6041248)(20161123558025)(20161123562025)(20161123560025)(20161123564025)(20161123555025)(6072148);SRVR:CY4PR08MB2871;BCL:0;PCL:0;RULEID:;SRVR:CY4PR08MB2871; 7, 49 -- x-forefront-prvs: 0235CBE7D0 7, 49 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10009020)(6009001)(7916002)(39410400002)(39830400002)(39450400003)(81166006)(74316002)(305945005)(122556002)(7736002)(189998001)(92566002)(3280700002)(2906002)(75432002)(88552002)(53936002)(3660700001)(2900100001)(2501003)(450100001)(86362001)(77096006)(8936002)(110136004)(38730400002)(8676002)(6506006)(50986999)(54356999)(7696004)(55016002)(102836003)(6116002)(6436002)(99286003)(5640700003)(2351001)(25786008)(6916009)(33656002)(5660300001)(9686003);DIR:OUT;SFP:1101;SCL:1;SRVR:CY4PR08MB2871;H:CY4PR08MB2869.namprd08.prod.outlook.com;FPR:;SPF:None;MLV:sfv;LANG:en; 7, 49 -- spamdiagnosticoutput: 1:99 7, 49 -- spamdiagnosticmetadata: NSPM 7, 49 -- Content-Type: text/plain; charset="us-ascii" 7, 49 -- MIME-Version: 1.0 7, 49 -- X-OriginatorOrg: csusb.edu 7, 49 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 03 Mar 2017 01:13:47.0310 7, 49 -- (UTC) 7, 49 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted 7, 49 -- X-MS-Exchange-CrossTenant-id: d73b9eaa-07c9-47c4-a6ce-f13bee0e8117 7, 49 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: CY4PR08MB2871 7, 49 -- Content-Transfer-Encoding: 8bit 7, 49 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v2316P03021393 ==============================End of - Headers==============================
Please feel free to send or post images to look at, but most likely you have a simple deflection problem. Remember that in SEM lenses are only used to form the beam, but magnification (and formation of image) comes from ratio between area physicall scanned by electron beam on surface of the sample and area of the CRT screen (or digital image) representing the image. If you see only vertical lines then chances are that primary electron beam is not scanned in Y direction over the sample, so in every line of image you are seeing information from the same exact line physically scanned by electron beam on the sample. Find a local tech who knows how to use oscilloscope and able to figure out amplifiers driving inductive loads (scan coils)....
Valery Ray www.linkedin.com/in/valeryray ============================== PBS&T, MEO Engineering Company 290 Broadway, Suite 298 Methuen, MA 01844, USA Phone: +1-978-296-5063 - leave a message Mobie: +1-978-305-0479 - leave a message E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 3/2/2017 8:07 PM, jthompso-at-csusb.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Our Hitachi S-2700 SEM will no longer produce an image. We can only see a series of vertical lines of varying intensity with both secondary electron and backscatter detectors. We do have an electron beam - there are changes in intensity of the vertical lines during adjustment of filament current, beam tilt, beam horizon, changes in the lines if we move the specimen. We do lose the image if the HV is turned off on the secondary electron detector. A very similar pattern is seen with the backscatter detector, so it does not appear to be a detector issue. } } We have exchanged circuit boards and even the entire column (!) from a second identical scope used for parts. A commercial technician was unable to correct the problem. His best guess is that one or more of the lenses are not functioning. } } Any suggestions as to what the problem is? I can send images off-line. } } } Jeffrey Thompson, Ph.D. } Professor of Biology } California State University } 5500 University Pkwy } San Bernardino, CA 92407 } 909-537-5315 } } } } ==============================Original Headers============================== } 7, 49 -- From jthompso-at-csusb.edu Thu Mar 2 19:06:25 2017 } 7, 49 -- Received: from NAM03-BY2-obe.outbound.protection.outlook.com (mail-by2nam03on0075.outbound.protection.outlook.com [104.47.42.75]) } 7, 49 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v2316P03021393 } 7, 49 -- for {Microscopy-at-microscopy.com} ; Thu, 2 Mar 2017 19:06:25 -0600 } 7, 49 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=csusb.edu; } 7, 49 -- s=selector1; h=From:Date:Subject:Message-ID:Content-Type:MIME-Version; } 7, 49 -- bh=2DXHl+sdv7lZfoI1ZlAV7dldcoThOfcItUM8cr4e4Yg=; } 7, 49 -- b=ItH5GzlWhXr5lMs9/qNc4uSnMCV7/fCa5nYZJSY1jKHojRCLtbNETh/vXuYiUmC/yoCWF1KbpA4Cm5gR5FhCj85Sn0OlkEwjp5nNW2gBMjGiqbU+42e0tzNtfK9lgXGFV+a/wyCFSdayxhfFkLeSEwfcwLizCNu58xUFMvn4Ikw= } 7, 49 -- Received: from CY4PR08MB2869.namprd08.prod.outlook.com (10.173.59.147) by } 7, 49 -- CY4PR08MB2871.namprd08.prod.outlook.com (10.173.59.149) with Microsoft SMTP } 7, 49 -- Server (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_256_CBC_SHA384_P384) id } 7, 49 -- 15.1.933.12; Fri, 3 Mar 2017 01:13:47 +0000 } 7, 49 -- Received: from CY4PR08MB2869.namprd08.prod.outlook.com ([10.173.59.147]) by } 7, 49 -- CY4PR08MB2869.namprd08.prod.outlook.com ([10.173.59.147]) with mapi id } 7, 49 -- 15.01.0933.020; Fri, 3 Mar 2017 01:13:47 +0000 } 7, 49 -- From: Jeffrey Thompson {jthompso-at-csusb.edu} } 7, 49 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} } 7, 49 -- Subject: SEM Need help in understanding loss of image generation } 7, 49 -- Thread-Topic: SEM Need help in understanding loss of image generation } 7, 49 -- Thread-Index: AdKTusYGL9em7MiDQpOEkIYERiIIyQ== } 7, 49 -- Date: Fri, 3 Mar 2017 01:13:47 +0000 } 7, 49 -- Message-ID: {CY4PR08MB286955557359A0BF5875540FCC2B0-at-CY4PR08MB2869.namprd08.prod.outlook.com} } 7, 49 -- Accept-Language: en-US } 7, 49 -- Content-Language: en-US } 7, 49 -- X-MS-Has-Attach: } 7, 49 -- X-MS-TNEF-Correlator: } 7, 49 -- authentication-results: microscopy.com; dkim=none (message not signed) } 7, 49 -- header.d=none;microscopy.com; dmarc=none action=none header.from=csusb.edu; } 7, 49 -- x-originating-ip: [2607:f380:a61:142:5583:ac34:b43d:3ef6] } 7, 49 -- x-ms-office365-filtering-correlation-id: 26778956-7cca-48ee-dd69-08d461d28b35 } 7, 49 -- x-microsoft-antispam: UriScan:;BCL:0;PCL:0;RULEID:(22001);SRVR:CY4PR08MB2871; } 7, 49 -- x-microsoft-exchange-diagnostics: 1;CY4PR08MB2871;7:rHS23JLozCsvFvo6phag6YemA56+clfV4rUPzDzQFY3NAqQSpVYtmBAOD5jSvxl8S/e2ourHvZApghyjoYRRY8Yq6cBuVKOQ6o8jHQ0tvKSqR9zGPKxCpmcmEGYSYLowGN9xDM0fS8yoHnpVwoH1ByQS0e1K442YN53nI3y/jv2Ur6xkOObcQmAUv+hhLNw0KRFWCxWLKM/zP0uQSui2YG8SOyekfRXzW33lZc1slb0y6obbxDehbW7027o/+FI+Tvrxbir+/dYbH6WHOi/8V+2SQsGEbPhRnlyktrO/TRTq4SNIAw3eWz62GreW3q3DMITuu7GTDqB9TAatF2jBLA==;20:FBEKbZT5i0wXScOUNyIugqVbQUjmHgFIfXKT2lgYME4k+z99AAECHORHR9o907/ivCzudIgqpsG6MDtbIXEhVqR5A7vJJZKpJogcHTCT4DokeVZLB1VDVrvyTTjAOj/BWXIpZwT4HKufvDYPtJMlQkpoyNXEHMcSu5q76tKAgjs= } 7, 49 -- x-microsoft-antispam-prvs: {CY4PR08MB2871632F020A7C5B5B32C212CC2B0-at-CY4PR08MB2871.namprd08.prod.outlook.com} } 7, 49 -- x-exchange-antispam-report-test: UriScan:; } 7, 49 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:(6040375)(601004)(2401047)(8121501046)(5005006)(3002001)(10201501046)(6041248)(20161123558025)(20161123562025)(20161123560025)(20161123564025)(20161123555025)(6072148);SRVR:CY4PR08MB2871;BCL:0;PCL:0;RULEID:;SRVR:CY4PR08MB2871; } 7, 49 -- x-forefront-prvs: 0235CBE7D0 } 7, 49 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10009020)(6009001)(7916002)(39410400002)(39830400002)(39450400003)(81166006)(74316002)(305945005)(122556002)(7736002)(189998001)(92566002)(3280700002)(2906002)(75432002)(88552002)(53936002)(3660700001)(2900100001)(2501003)(450100001)(86362001)(77096006)(8936002)(110136004)(38730400002)(8676002)(6506006)(50986999)(54356999)(7696004)(55016002)(102836003)(6116002)(6436002)(99286003)(5640700003)(2351001)(25786008)(6916009)(33656002)(5660300001)(9686003);DIR:OUT;SFP:1101;SCL:1;SRVR:CY4PR08MB2871;H:CY4PR08MB2869.namprd08.prod.outlook.com;FPR:;SPF:None;MLV:sfv;LANG:en; } 7, 49 -- spamdiagnosticoutput: 1:99 } 7, 49 -- spamdiagnosticmetadata: NSPM } 7, 49 -- Content-Type: text/plain; charset="us-ascii" } 7, 49 -- MIME-Version: 1.0 } 7, 49 -- X-OriginatorOrg: csusb.edu } 7, 49 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 03 Mar 2017 01:13:47.0310 } 7, 49 -- (UTC) } 7, 49 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted } 7, 49 -- X-MS-Exchange-CrossTenant-id: d73b9eaa-07c9-47c4-a6ce-f13bee0e8117 } 7, 49 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: CY4PR08MB2871 } 7, 49 -- Content-Transfer-Encoding: 8bit } 7, 49 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v2316P03021393 } ==============================End of - Headers============================== }
==============================Original Headers============================== 4, 35 -- From vray-at-partbeamsystech.com Thu Mar 2 20:48:39 2017 4, 35 -- Received: from nm26-vm0.bullet.mail.bf1.yahoo.com (nm26-vm0.bullet.mail.bf1.yahoo.com [98.139.213.74]) 4, 35 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v232mdq0027214 4, 35 -- for {microscopy-at-microscopy.com} ; Thu, 2 Mar 2017 20:48:39 -0600 4, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1488509762; bh=mQrACGkKDk5yOn+jsNbiKZ8nc58U3hL2mdQoBRyZo9o=; h=Subject:To:References:From:Date:In-Reply-To:From:Subject; b=TW0wXGuxztQ9CuYpgw+j/bBMPZNqRQ9IN+xEYC+srrrkl5oHt2Scs+wJXYPzYYSDTL+P+JiT0cP/45pidG3Q8FIASUV3Emts0KsloFf1SEB1bnx7ip4yCOzIxxDBs65ZVHoG51sqkg4pzw5nb/0U3Ltlj/WFwALV1aJdCu/NahJE98a0BoRwfBdmsvqNk1GEy0B1umObhTH1IyVHmH2gTKFjCWQuakeZdlYx7ctf8Ty5seB1WALVcHNRfnjKu62krVtsSPe9zQOWPKCrK6OqhC2IZDhKTZez+CRTPPF9XDwJALL152WSLSwds8lZ47jpTVuG009GCVMS/f19bpVDvw== 4, 35 -- Received: from [66.196.81.174] by nm26.bullet.mail.bf1.yahoo.com with NNFMP; 03 Mar 2017 02:56:02 -0000 4, 35 -- Received: from [98.139.213.8] by tm20.bullet.mail.bf1.yahoo.com with NNFMP; 03 Mar 2017 02:56:02 -0000 4, 35 -- Received: from [127.0.0.1] by smtp108.mail.bf1.yahoo.com with NNFMP; 03 Mar 2017 02:56:02 -0000 4, 35 -- X-Yahoo-Newman-Id: 136647.76206.bm-at-smtp108.mail.bf1.yahoo.com 4, 35 -- X-Yahoo-Newman-Property: ymail-3 4, 35 -- X-YMail-OSG: J6z.gvsVM1krMH39CRY9cvZGHNMCOzY6Bh7AOLbO745Nvwk 4, 35 -- cutEivUnh.ECfYvAoBCOwQu08KMYGo3V.rACPZoAxgIE67hY9gzAFBqRL6bd 4, 35 -- Xq.AtrYVqzlrk5epFGjlD1J4KJEKtrorvVbWYbWFAVhloWtmkPZ7PkAkeVsR 4, 35 -- 9jWzQi_Kog3QTO8ASSCsN97n0xsH7QbPVlIPkKNi18IbEWviaBxSEzXgG1sC 4, 35 -- PvxounFKM9wtlznGD3xepC5ZSJzoGWQQiBcMuUAHAvzwxc.0ZA05itbcAkEn 4, 35 -- sX_Cc21ucDPM_UeBWlCcpdxLzVo1z9H5HpXMRKquvjK45gWUjxFzQLLiUg9H 4, 35 -- SgMKg9MT5ka3LEJ40mBpFWtXXH.0781TU1A6LXp.eb.SxdWfhS0LDjSmbMXY 4, 35 -- K8cuL2GiYtYws9EXWj5tEFKtxcOPamm1XJgDCt7gZqhK9ps9wphGMIcyUkRG 4, 35 -- 9y7QNYy0NWeviMbhMsk2huFQGw6FqO5On8EmsjSh5h7KSBav_T1V4n7Vo98A 4, 35 -- UDFv_hnUUwJBDu5l1xJbJt9VTMz7zesiSBISRNhNkohGYBGIQOMtHzH7zlkl 4, 35 -- GE9VGv6hMCA-- 4, 35 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 4, 35 -- Subject: Re: [Microscopy] SEM Need help in understanding loss of image 4, 35 -- generation 4, 35 -- To: jthompso-at-csusb.edu, microscopy-at-microscopy.com 4, 35 -- References: {201703030107.v2317uxT023818-at-microscopy.com} 4, 35 -- From: Valery Ray {vray-at-partbeamsystech.com} 4, 35 -- Message-ID: {5df6df2a-21b3-0e8e-beed-d299f7bf89c9-at-partbeamsystech.com} 4, 35 -- Date: Thu, 2 Mar 2017 21:52:12 -0500 4, 35 -- User-Agent: Mozilla/5.0 (Windows NT 10.0; WOW64; rv:45.0) Gecko/20100101 4, 35 -- Thunderbird/45.7.1 4, 35 -- MIME-Version: 1.0 4, 35 -- In-Reply-To: {201703030107.v2317uxT023818-at-microscopy.com} 4, 35 -- Content-Type: text/plain; charset=windows-1252; format=flowed 4, 35 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both erin-at-boeckeler.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Message: 4.5-day workshop with both hands-on and lecture sessions for cryo-sample preparation in the life sciences and material sciences. Participants can bring their own samples for one-on-one assistance.
Details: April 17-21, 2017 RMC Boeckeler Facility Tucson, AZ, USA
Instruments at the facility include a TEM, fume hood & 'wet lab' area, ultramicrotomes, cryosectioning systems, microtomes, automated stainer, optical microscopes, plus all needed accessories.
Class size is limited! Register by March 24, 2017. Cost covers the workshop, plus hotel accommodations, meals, and transportation to and from the hotel.
For information visit http://www.rmcboeckeler.com/download/Cryo_in_the_Sun/CITS_flyer.pdf
Login Host: 68.98.221.238 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
Hi Jeffrey, I'm agree with Valery, a deflection circuit is faulty. A deflection problem is also possible from the screen circuit. This can be check by connecting the video output on an external monitor. This microscope has probably two stages of deflectors to scan the beam and each of them set with X and Y deflection coils. The most common problem is coming from power transistor on horizontal circuit. This component is more sollicited because the scan speed horizontal is faster than the vertical. This electronic is sensitive to temperature of the room and of the cooling water. The fastest is the speed the more those transistors are working, if you can set your microscope to slow scan mode when you don't use, it's safety for deflection circuit. High magnification position is also good.
Nicolas STEPHANT
Universit de Nantes Institut Jean Rouxel Service de microscopie lectronique balayage et microanalyse 2 rue de la Houssinire BP 92208 44322 Nantes cdex 3
Tl : 02 40 37 64 26 Ml : nicolas.stephant-at-univ-nantes.fr Web : http://cnrs-imn.fr
"Le monde n'existe que pour autant que nous sommes capables d'en produire une image" C.G Jung
Le 03/03/2017 02:21, jthompso-at-csusb.edu a crit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Our Hitachi S-2700 SEM will no longer produce an image. We can only see a series of vertical lines of varying intensity with both secondary electron and backscatter detectors. We do have an electron beam - there are changes in intensity of the vertical lines during adjustment of filament current, beam tilt, beam horizon, changes in the lines if we move the specimen. We do lose the image if the HV is turned off on the secondary electron detector. A very similar pattern is seen with the backscatter detector, so it does not appear to be a detector issue. } } We have exchanged circuit boards and even the entire column (!) from a second identical scope used for parts. A commercial technician was unable to correct the problem. His best guess is that one or more of the lenses are not functioning. } } Any suggestions as to what the problem is? I can send images off-line. } } } Jeffrey Thompson, Ph.D. } Professor of Biology } California State University } 5500 University Pkwy } San Bernardino, CA 92407 } 909-537-5315 } } } } ==============================Original Headers============================== } 7, 49 -- From jthompso-at-csusb.edu Thu Mar 2 19:06:25 2017 } 7, 49 -- Received: from NAM03-BY2-obe.outbound.protection.outlook.com (mail-by2nam03on0075.outbound.protection.outlook.com [104.47.42.75]) } 7, 49 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v2316P03021393 } 7, 49 -- for {Microscopy-at-microscopy.com} ; Thu, 2 Mar 2017 19:06:25 -0600 } 7, 49 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=csusb.edu; } 7, 49 -- s=selector1; h=From:Date:Subject:Message-ID:Content-Type:MIME-Version; } 7, 49 -- bh=2DXHl+sdv7lZfoI1ZlAV7dldcoThOfcItUM8cr4e4Yg=; } 7, 49 -- b=ItH5GzlWhXr5lMs9/qNc4uSnMCV7/fCa5nYZJSY1jKHojRCLtbNETh/vXuYiUmC/yoCWF1KbpA4Cm5gR5FhCj85Sn0OlkEwjp5nNW2gBMjGiqbU+42e0tzNtfK9lgXGFV+a/wyCFSdayxhfFkLeSEwfcwLizCNu58xUFMvn4Ikw= } 7, 49 -- Received: from CY4PR08MB2869.namprd08.prod.outlook.com (10.173.59.147) by } 7, 49 -- CY4PR08MB2871.namprd08.prod.outlook.com (10.173.59.149) with Microsoft SMTP } 7, 49 -- Server (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_256_CBC_SHA384_P384) id } 7, 49 -- 15.1.933.12; Fri, 3 Mar 2017 01:13:47 +0000 } 7, 49 -- Received: from CY4PR08MB2869.namprd08.prod.outlook.com ([10.173.59.147]) by } 7, 49 -- CY4PR08MB2869.namprd08.prod.outlook.com ([10.173.59.147]) with mapi id } 7, 49 -- 15.01.0933.020; Fri, 3 Mar 2017 01:13:47 +0000 } 7, 49 -- From: Jeffrey Thompson {jthompso-at-csusb.edu} } 7, 49 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} } 7, 49 -- Subject: SEM Need help in understanding loss of image generation } 7, 49 -- Thread-Topic: SEM Need help in understanding loss of image generation } 7, 49 -- Thread-Index: AdKTusYGL9em7MiDQpOEkIYERiIIyQ== } 7, 49 -- Date: Fri, 3 Mar 2017 01:13:47 +0000 } 7, 49 -- Message-ID: {CY4PR08MB286955557359A0BF5875540FCC2B0-at-CY4PR08MB2869.namprd08.prod.outlook.com} } 7, 49 -- Accept-Language: en-US } 7, 49 -- Content-Language: en-US } 7, 49 -- X-MS-Has-Attach: } 7, 49 -- X-MS-TNEF-Correlator: } 7, 49 -- authentication-results: microscopy.com; dkim=none (message not signed) } 7, 49 -- header.d=none;microscopy.com; dmarc=none action=none header.from=csusb.edu; } 7, 49 -- x-originating-ip: [2607:f380:a61:142:5583:ac34:b43d:3ef6] } 7, 49 -- x-ms-office365-filtering-correlation-id: 26778956-7cca-48ee-dd69-08d461d28b35 } 7, 49 -- x-microsoft-antispam: UriScan:;BCL:0;PCL:0;RULEID:(22001);SRVR:CY4PR08MB2871; } 7, 49 -- x-microsoft-exchange-diagnostics: 1;CY4PR08MB2871;7:rHS23JLozCsvFvo6phag6YemA56+clfV4rUPzDzQFY3NAqQSpVYtmBAOD5jSvxl8S/e2ourHvZApghyjoYRRY8Yq6cBuVKOQ6o8jHQ0tvKSqR9zGPKxCpmcmEGYSYLowGN9xDM0fS8yoHnpVwoH1ByQS0e1K442YN53nI3y/jv2Ur6xkOObcQmAUv+hhLNw0KRFWCxWLKM/zP0uQSui2YG8SOyekfRXzW33lZc1slb0y6obbxDehbW7027o/+FI+Tvrxbir+/dYbH6WHOi/8V+2SQsGEbPhRnlyktrO/TRTq4SNIAw3eWz62GreW3q3DMITuu7GTDqB9TAatF2jBLA==;20:FBEKbZT5i0wXScOUNyIugqVbQUjmHgFIfXKT2lgYME4k+z99AAECHORHR9o907/ivCzudIgqpsG6MDtbIXEhVqR5A7vJJZKpJogcHTCT4DokeVZLB1VDVrvyTTjAOj/BWXIpZwT4HKufvDYPtJMlQkpoyNXEHMcSu5q76tKAgjs= } 7, 49 -- x-microsoft-antispam-prvs: {CY4PR08MB2871632F020A7C5B5B32C212CC2B0-at-CY4PR08MB2871.namprd08.prod.outlook.com} } 7, 49 -- x-exchange-antispam-report-test: UriScan:; } 7, 49 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:(6040375)(601004)(2401047)(8121501046)(5005006)(3002001)(10201501046)(6041248)(20161123558025)(20161123562025)(20161123560025)(20161123564025)(20161123555025)(6072148);SRVR:CY4PR08MB2871;BCL:0;PCL:0;RULEID:;SRVR:CY4PR08MB2871; } 7, 49 -- x-forefront-prvs: 0235CBE7D0 } 7, 49 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10009020)(6009001)(7916002)(39410400002)(39830400002)(39450400003)(81166006)(74316002)(305945005)(122556002)(7736002)(189998001)(92566002)(3280700002)(2906002)(75432002)(88552002)(53936002)(3660700001)(2900100001)(2501003)(450100001)(86362001)(77096006)(8936002)(110136004)(38730400002)(8676002)(6506006)(50986999)(54356999)(7696004)(55016002)(102836003)(6116002)(6436002)(99286003)(5640700003)(2351001)(25786008)(6916009)(33656002)(5660300001)(9686003);DIR:OUT;SFP:1101;SCL:1;SRVR:CY4PR08MB2871;H:CY4PR08MB2869.namprd08.prod.outlook.com;FPR:;SPF:None;MLV:sfv;LANG:en; } 7, 49 -- spamdiagnosticoutput: 1:99 } 7, 49 -- spamdiagnosticmetadata: NSPM } 7, 49 -- Content-Type: text/plain; charset="us-ascii" } 7, 49 -- MIME-Version: 1.0 } 7, 49 -- X-OriginatorOrg: csusb.edu } 7, 49 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 03 Mar 2017 01:13:47.0310 } 7, 49 -- (UTC) } 7, 49 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted } 7, 49 -- X-MS-Exchange-CrossTenant-id: d73b9eaa-07c9-47c4-a6ce-f13bee0e8117 } 7, 49 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: CY4PR08MB2871 } 7, 49 -- Content-Transfer-Encoding: 8bit } 7, 49 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v2316P03021393 } ==============================End of - Headers==============================
==============================Original Headers============================== 7, 26 -- From Nicolas.Stephant-at-univ-nantes.fr Fri Mar 3 02:02:10 2017 7, 26 -- Received: from mail2.cnrs-imn.fr (mail2.cnrs-imn.fr [193.52.97.4]) 7, 26 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v23829Bi015455 7, 26 -- for {microscopy-at-microscopy.com} ; Fri, 3 Mar 2017 02:02:10 -0600 7, 26 -- Received: from pro-smtp.cnrs-imn.fr (smtp.cnrs-imn.fr [194.214.57.230]) 7, 26 -- by mail2.cnrs-imn.fr (8.14.4/8.14.4/DG) with ESMTP id v2389TKR005436; 7, 26 -- Fri, 3 Mar 2017 09:09:29 +0100 7, 26 -- Received: from [10.2.6.11] (pc11.cnrs-imn.fr [10.2.6.11]) 7, 26 -- by pro-smtp.cnrs-imn.fr (8.14.4/8.14.4/Debian-8+deb8u1) with ESMTP id v2389SgX071606 7, 26 -- (version=TLSv1/SSLv3 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128 verify=NOT); 7, 26 -- Fri, 3 Mar 2017 09:09:29 +0100 7, 26 -- Reply-To: Nicolas.Stephant-at-univ-nantes.fr 7, 26 -- Subject: Re: SEM Need help in understanding loss of image generation 7, 26 -- References: {201703030121.v231Llu4014233-at-microscopy.com} 7, 26 -- To: jthompso-at-csusb.edu, microscopy-at-microscopy.com 7, 26 -- From: Nicolas Stephant {Nicolas.Stephant-at-univ-nantes.fr} 7, 26 -- Message-ID: {4f7a1e0d-7a2f-60ea-6120-ba90d0e9fbb3-at-univ-nantes.fr} 7, 26 -- Date: Fri, 3 Mar 2017 09:09:59 +0100 7, 26 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:45.0) Gecko/20100101 7, 26 -- Thunderbird/45.7.0 7, 26 -- MIME-Version: 1.0 7, 26 -- In-Reply-To: {201703030121.v231Llu4014233-at-microscopy.com} 7, 26 -- Content-Type: text/plain; charset=windows-1252; format=flowed 7, 26 -- Content-Transfer-Encoding: 8bit 7, 26 -- X-Miltered: at b-mail2 with ID 58B924B9.000 by Joe's j-chkmail (http : // j-chkmail dot ensmp dot fr)! 7, 26 -- X-j-chkmail-Enveloppe: 58B924B9.000 from smtp.cnrs-imn.fr/smtp.cnrs-imn.fr/194.214.57.230/pro-smtp.cnrs-imn.fr/ {Nicolas.Stephant-at-univ-nantes.fr} ==============================End of - Headers==============================
Dear Listers, a friend of mine is interested in buying a SEM with a LaB6 emitter, for imaging and, above all, analytical applications: EDS and WDS. However, he was told that LaB6 emitters are not a good choice for analytical applications in a SEM, mainly due to stability issues, which - if I got it correctly - would require a long wait time before reliable spectra could be acquired. I have no experience with such a machine, so I can't advice him about this. Maybe some of you can help me. Thank you in advance. Regards
==============================Original Headers============================== 6, 46 -- From dcristofori-at-unive.it Fri Mar 3 10:22:45 2017 6, 46 -- Received: from mail-wr0-f173.google.com (mail-wr0-f173.google.com [209.85.128.173]) 6, 46 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v23GMiPM018377 6, 46 -- for {microscopy-at-microscopy.com} ; Fri, 3 Mar 2017 10:22:45 -0600 6, 46 -- Received: by mail-wr0-f173.google.com with SMTP id u48so77244866wrc.0 6, 46 -- for {microscopy-at-microscopy.com} ; Fri, 03 Mar 2017 08:30:10 -0800 (PST) 6, 46 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 46 -- d=unive-it.20150623.gappssmtp.com; s=20150623; 6, 46 -- h=from:to:subject:message-id:date:user-agent:mime-version 6, 46 -- :content-transfer-encoding; 6, 46 -- bh=QQOwdriEmZR1wQYL8Gco610AhFpwwY9mqzxT0DxSf8M=; 6, 46 -- b=M49XawHtmLofs3N57L/N3GtPKhNdWKKnwVPh6oYDlLbkg34ryQPc3BC3herZ1yGK9I 6, 46 -- e+wrlpyVJFNcrjXwPaOtmvZJHH7F/6/lNmEhtdd3AFOENh9wljyDptP5S+bjQJeSvbm/ 6, 46 -- 6QTw0Nc/selXiqeH1po4zQ3QIOSFR5vaueckyZJzzdCqNSITVLPO2s3A+5TRoFke+QG4 6, 46 -- ZUVykpzfMbKAfqGO+ASJGSv07iDoEU5LFUOHPp175+i1Eh8/PxIuHCNPKZPxGisBHiYY 6, 46 -- M39UKVSDwubwbe2hev5c7FGhW468hmLAh2zfkNaTyDcTDHPw4qZ/xFJbSZZXvgQ17v4I 6, 46 -- Gd9A== 6, 46 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 46 -- d=1e100.net; s=20161025; 6, 46 -- h=x-gm-message-state:from:to:subject:message-id:date:user-agent 6, 46 -- :mime-version:content-transfer-encoding; 6, 46 -- bh=QQOwdriEmZR1wQYL8Gco610AhFpwwY9mqzxT0DxSf8M=; 6, 46 -- b=jvGpQU1r86GyTGoj9JEP6GuJVM6Fr6hzl00gZWgI6knwDUsi49Zh+gQDGUPLcQm6v0 6, 46 -- s3dEiXIkEDtSbDoGvQxU+SZtDZX06iRr6NTr/rP6s9tinSGYOgHUiLKLHJm831xOKiXJ 6, 46 -- B/Q9QwpcU0sZEXWuNx77EZ5h8l3w5QmzlQNhwch3WwAozPCsr+aFF2uW8EK3EBs5Dxhu 6, 46 -- hl/jR7cbomQTPmXJVqomFysTgRwjzG9p04HACpYiWgSLLe0DKAxks9CdUDMhkEj360sx 6, 46 -- Kg2MNVroj4vz9moMa4u7H3qyZCCfJFLtXAih474cQIr+LPeXV96LLLheNNiKjiNauNgn 6, 46 -- /k6A== 6, 46 -- X-Gm-Message-State: AMke39kFDIoB48acGUfpQ1fhWSsUYWTnwUsJSh8w+5Y9SvmFAH/VhdNTvaev9nEd7hKvbagf 6, 46 -- X-Received: by 10.223.153.17 with SMTP id x17mr3372369wrb.55.1488558608506; 6, 46 -- Fri, 03 Mar 2017 08:30:08 -0800 (PST) 6, 46 -- Received: from [157.138.23.17] (battagliarin.dcf.unive.it. [157.138.23.17]) 6, 46 -- by smtp.gmail.com with ESMTPSA id 11sm15805757wrb.10.2017.03.03.08.30.06 6, 46 -- for {microscopy-at-microscopy.com} 6, 46 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 6, 46 -- Fri, 03 Mar 2017 08:30:07 -0800 (PST) 6, 46 -- From: Davide Cristofori {dcristofori-at-unive.it} 6, 46 -- To: microscopy-at-microscopy.com 6, 46 -- Subject: SEM: are LaB6 emitters fit for EDS and WDS? 6, 46 -- Message-ID: {b75d8a9c-5ac7-9147-523f-d53f3c49537f-at-unive.it} 6, 46 -- Date: Fri, 3 Mar 2017 17:30:31 +0100 6, 46 -- User-Agent: Mozilla/5.0 (Windows NT 10.0; WOW64; rv:45.0) Gecko/20100101 6, 46 -- Thunderbird/45.7.1 6, 46 -- MIME-Version: 1.0 6, 46 -- Content-Type: text/plain; charset=iso-8859-15; format=flowed 6, 46 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
From medinaluca1-at-gmail.com Fri Mar 3 16:42:07 2017 Return-Path: {medinaluca1-at-gmail.com} Received: from gmail.com (61-222-131-125.HINET-IP.hinet.net [61.222.131.125]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v23Mg4Dt021132 for {microscopylistserverarchive6-at-microscopy.com} ; Fri, 3 Mar 2017 16:42:06 -0600 Message-ID: {8F6BD8DD.8098AF46-at-gmail.com}
Hi
I know of no problems with LaB6 emitters with regard to stability, provide that the vacuum level in the electron gun is suitable for their use. Could you be confusing the instability problem with cold field emitter instruments, where their natural emission is prone to early and late instabilities during long operating session. Regards
Steve
Steve Chapman FRMS Protrain for Consultancy and Training in Electron Microscopy +44 (0)7711 606967 web www.emcourses.com
-----Original Message----- X-from: dcristofori-at-unive.it [mailto:dcristofori-at-unive.it] Sent: 03 March 2017 16:24 To: protrain-at-emcourses.com
Dear Listers, a friend of mine is interested in buying a SEM with a LaB6 emitter, for imaging and, above all, analytical applications: EDS and WDS. However, he was told that LaB6 emitters are not a good choice for analytical applications in a SEM, mainly due to stability issues, which - if I got it correctly - would require a long wait time before reliable spectra could be acquired. I have no experience with such a machine, so I can't advice him about this. Maybe some of you can help me. Thank you in advance. Regards
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both roseann.csencsits-at-schafercorp.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Northern California Society for Microscopy Meeting March 16
Message: MSA Fellow and Featured speaker: Professor William Landis
"A possible role in vertebrate mineralization for the small, non-collagenous protein, osteocalcin, as determined by immunocytochemistry"
Lecture includes: standard and high voltage TEM, computer simulation, immunocytochemistry, and nucleation and growth of Hydroxyapatite
TEM, biology, and materials science - 3 in one! For what more could one ask?
Hosted by EAG, 810 Kifer Road, Sunnyvale, CA Thursday March 16, 6-9 pm
RSVP: info-at-ncsmicroscopy.org by March 7. More information at www.ncsmicroscopy.org
Login Host: 99.65.181.103 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
Microscopy-at-Microscopy.com
============================================
==============================Original Headers============================== 18, 52 -- From microscopy.listserver-at-gmail.com Sat Mar 4 07:35:25 2017 18, 52 -- Received: from mail-it0-f47.google.com (mail-it0-f47.google.com [209.85.214.47]) 18, 52 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v24DZPJ0003692 18, 52 -- for {microscopy-at-microscopy.com} ; Sat, 4 Mar 2017 07:35:25 -0600 18, 52 -- Received: by mail-it0-f47.google.com with SMTP id 203so28294826ith.0 18, 52 -- for {microscopy-at-microscopy.com} ; Sat, 04 Mar 2017 05:42:54 -0800 (PST) 18, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 18, 52 -- d=gmail.com; s=20161025; 18, 52 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 18, 52 -- :mime-version:in-reply-to:content-transfer-encoding; 18, 52 -- bh=jv/nqZRi+yQxFGWANqWo63qSRVjLQNiZqjY9E+KJaAc=; 18, 52 -- b=XDHyXhULcYQ/yhQNHvdp85V6pcDVGC89v0l5SS7KRU3ZIeF8V04tYJAI+cyFJa7/FS 18, 52 -- 4ERLWalc7S4/yXRLwCA9LzHVHGDc6usKns+DsnGTq3oiWro9iVA+oVugVWjqNLm8jzJc 18, 52 -- TRtPuto1GQgtLpDJITYzOEj0MIR0RTdRP8RUzPhTatBCN8CgNEC3NCypN5Yf40FU2YxB 18, 52 -- i3c9CdGcvOo452pRj/XxBetANhKQ30B/LcqGxGe7nL+RXyvcjbbjHhqvCZWSUC2ekZT+ 18, 52 -- 52WPPc6aLMdMvVOYBWWahy+cSTrqEAq2xKPggSyq3AUVgbGZbRVKWIZs1uI4uuHGulVh 18, 52 -- cPBA== 18, 52 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 18, 52 -- d=1e100.net; s=20161025; 18, 52 -- h=x-gm-message-state:from:subject:references:to:reply-to:message-id 18, 52 -- :date:user-agent:mime-version:in-reply-to:content-transfer-encoding; 18, 52 -- bh=jv/nqZRi+yQxFGWANqWo63qSRVjLQNiZqjY9E+KJaAc=; 18, 52 -- b=RtJ5Kj7KwqKycIFnm3ATgXQz14Wy28HcJBZosr0V7Ge8paXuB76B8BEk8N52GrvkxZ 18, 52 -- a8u51K/ZxtIW/lSDJvvahEnfb/mZ2s/gu+7nDjeyfyhCezDIKqocR9/oCE3Sc+Y7TjvA 18, 52 -- p1tfKwnZfluwBGD+cUDXknb2FQmrtWyhGXknNwzVfsFvD/FBlH5aFCkqFlgtEe/ecx/a 18, 52 -- 6xTqr20GRtsIZ1hhl70gRPKUTP31PhGr07lVIz3Jc2ucy6jFDpJBOmd/T010zgkGvHzR 18, 52 -- Zb64Qf5jHZIUHl9VNCRCl3Ac7gTR7SlbPK2SmzjqjQ3On0fhtSbWZ4W4jK1kFPxVgLWf 18, 52 -- 2weg== 18, 52 -- X-Gm-Message-State: AMke39kNa5lwkBo5xmkij2Wycj8IyLIStv/FuF5fY3C1MxFJu0y5zwDkQ8DI+2xLlJu8xQ== 18, 52 -- X-Received: by 10.36.102.195 with SMTP id k186mr7831255itc.75.1488634973515; 18, 52 -- Sat, 04 Mar 2017 05:42:53 -0800 (PST) 18, 52 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:9f0:b371:2ad9:786f]) 18, 52 -- by smtp.googlemail.com with ESMTPSA id o191sm5848379iod.11.2017.03.04.05.42.52 18, 52 -- for {microscopy-at-microscopy.com} 18, 52 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 18, 52 -- Sat, 04 Mar 2017 05:42:52 -0800 (PST) 18, 52 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 18, 52 -- X-Google-Original-From: MicroscopyListserver-NoReply 18, 52 -- {microscopylistserver-noreply-at-microscopy.com} 18, 52 -- Subject: viaWWW:Northern California Society for Microscopy Meeting March 16 18, 52 -- References: {201703040406.v2446cWG029113-at-microscopy.com} 18, 52 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 18, 52 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 18, 52 -- X-Forwarded-Message-Id: {201703040406.v2446cWG029113-at-microscopy.com} 18, 52 -- Message-ID: {7b14054d-8243-0acb-50bc-596aa9b0a8a7-at-microscopy.com} 18, 52 -- Date: Sat, 4 Mar 2017 07:42:51 -0600 18, 52 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:45.0) 18, 52 -- Gecko/20100101 Thunderbird/45.7.1 18, 52 -- MIME-Version: 1.0 18, 52 -- In-Reply-To: {201703040406.v2446cWG029113-at-microscopy.com} 18, 52 -- Content-Type: text/plain; charset=windows-1252; format=flowed 18, 52 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Dear List I am looking for SEM images of the various types/states of human white blood cells. Does anybody know any accessible source of such images that is scientifically sound? Thanks for your time yorgos
Tel/fax +30 210 8957677 mobile +30 6945 107477 www.eikonika.netwww.aim.cat *************************************
==============================Original Headers============================== 5, 20 -- From eikonika-at-otenet.gr Sun Mar 5 01:52:51 2017 5, 20 -- Received: from echidna.otenet.gr (smtp-out33.otenet.gr [83.235.69.33]) 5, 20 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v257qp7s009600 5, 20 -- for {microscopy-at-microscopy.com} ; Sun, 5 Mar 2017 01:52:51 -0600 5, 20 -- Received: from ozymandias (ppp-2-84-169-220.home.otenet.gr [2.84.169.220]) 5, 20 -- by echidna.otenet.gr (ESMTP) with ESMTPSA 5, 20 -- for {microscopy-at-microscopy.com} ; Sun, 5 Mar 2017 10:00:19 +0200 (EET) 5, 20 -- From: "Yorgos Nikas" {eikonika-at-otenet.gr} 5, 20 -- To: {microscopy-at-microscopy.com} 5, 20 -- Subject: leucocyte types in SEM 5, 20 -- Date: Sun, 5 Mar 2017 10:00:21 +0200 5, 20 -- Message-ID: {000001d29586$89f24d00$9dd6e700$-at-otenet.gr} 5, 20 -- MIME-Version: 1.0 5, 20 -- Content-Type: text/plain; 5, 20 -- charset="iso-8859-1" 5, 20 -- X-Mailer: Microsoft Outlook 14.0 5, 20 -- Thread-Index: AdKVhaNEwTFdRgEUSHmWLgMsN4X4yQ== 5, 20 -- Content-Language: en-us 5, 20 -- Content-Transfer-Encoding: 8bit 5, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v257qp7s009600 ==============================End of - Headers==============================
From donahenr845-at-gmail.com Mon Mar 6 03:48:07 2017 Return-Path: {donahenr845-at-gmail.com} Received: from gmail.com ([220.125.146.235]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v269m4la008671 for {microscopylistserverarchive6-at-microscopy.com} ; Mon, 6 Mar 2017 03:48:06 -0600 Message-ID: {912986DA.0519D8D0-at-gmail.com}
X-from: kuryu-at-rockefeller.edu
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both kuryu-at-rockefeller.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: kuryu-at-rockefeller.edu Name: Hiro Uryu
Organization: rockefeller university
Title-Subject: [Filtered] Do you know about these electon dese bodies in ER?
Message: Dear list,
I was searching for a significance of electron dense bodies that appeared to be spherical and located inside of ER. These may be heavily electron dense with or without an electron opaque core. So far I found one image in the following link, indicting a type of structured that I have seen. http://classes.kumc.edu/som/CellBiology/organelles/smoother/index.html Does anyone have an idea what they might be and what the biological significance might be associated with? I would love to hear your understanding of these dense bodies. I would be happy to share my image if you would connect with me off site. Many thanks,
Login Host: 129.85.110.225 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
Microscopy-at-Microscopy.com
============================================
==============================Original Headers============================== 13, 52 -- From microscopy.listserver-at-gmail.com Tue Mar 7 07:14:33 2017 13, 52 -- Received: from mail-it0-f43.google.com (mail-it0-f43.google.com [209.85.214.43]) 13, 52 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v27DEW4e010261 13, 52 -- for {microscopy-at-microscopy.com} ; Tue, 7 Mar 2017 07:14:32 -0600 13, 52 -- Received: by mail-it0-f43.google.com with SMTP id m27so10828644iti.1 13, 52 -- for {microscopy-at-microscopy.com} ; Tue, 07 Mar 2017 05:22:12 -0800 (PST) 13, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 13, 52 -- d=gmail.com; s=20161025; 13, 52 -- h=from:subject:references:reply-to:to:message-id:date:user-agent 13, 52 -- :mime-version:in-reply-to:content-transfer-encoding; 13, 52 -- bh=v9uxovmvMtjum/IJ3wVAs/8Qkgy2PeSq9zaFDVBhenQ=; 13, 52 -- b=NF+QX8CAGbPXl+qVCTmP2076JfEN9V7Ucn1hXfodg/yE/5dz+tildkliOF1Bpz8VWh 13, 52 -- BtVSfhHwYHOS1hBzNkeRNS1VMYh/x0szVd2F0PmT+oePK4AIS85hsv7b2jG2T7LZy3LT 13, 52 -- 4FVSc+R5rgc14wi9f9vA73+qjqPr1TwsOor7LlAEs3D/Ztm9XTCiyivU/gl6a0bcIKWG 13, 52 -- ZcUb9/sWoeeXVlvPBUPEh8PeyuZfKJoEkOcR3fCTdg4U/BkmMggTiZv9JW/OP82+jAG7 13, 52 -- Cp8Z0M6yAXr4H5UOTSCSHFxCVM0WHlJ8JlbB6rP5PEw5KbVQLMKjeUzdYfIO/b0SXlIl 13, 52 -- A2AA== 13, 52 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 13, 52 -- d=1e100.net; s=20161025; 13, 52 -- h=x-gm-message-state:from:subject:references:reply-to:to:message-id 13, 52 -- :date:user-agent:mime-version:in-reply-to:content-transfer-encoding; 13, 52 -- bh=v9uxovmvMtjum/IJ3wVAs/8Qkgy2PeSq9zaFDVBhenQ=; 13, 52 -- b=mQj0uyxuvjzzMEbt23ifQuIvGKQnVBhYv0WgnRt9MbXGjH6tuWsXHrVV1gVip7Blg3 13, 52 -- mDI2vcYLCSVmJty56HKS8YHy4SRq7Qfw9nysQlJt4u+lkj1Hx6FoVHtxLB8GYwrWIePI 13, 52 -- p0clfBEB1bToku0oyR/HW5Ma0BOVpP0X/ysJ4xOoMxRueZzcG49K1VZoJLKmWT4gW+QD 13, 52 -- CuJvi+h0ZwqmddVnhZ8yukYOHqCp7wbFCdNTBeOvIfM+O7ieiK+yvybFKcu2lKPYQDw8 13, 52 -- FRb63GttxMK3J50PRztqL7j/VthltSGgqOf6+i6wBvQjmMsfKqpEHcYQ3KBcR2BchB6v 13, 52 -- EnQQ== 13, 52 -- X-Gm-Message-State: AMke39ke6wCv7OhkYMwXr3n27I+LkQAtbLJP/5R3+CHCAVG00EpTLm749Yf7MkS0Be8X2g== 13, 52 -- X-Received: by 10.36.152.196 with SMTP id n187mr729576itd.28.1488892931657; 13, 52 -- Tue, 07 Mar 2017 05:22:11 -0800 (PST) 13, 52 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:6c3f:4509:ab21:9590]) 13, 52 -- by smtp.googlemail.com with ESMTPSA id k68sm127790iod.13.2017.03.07.05.22.10 13, 52 -- for {microscopy-at-microscopy.com} 13, 52 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 13, 52 -- Tue, 07 Mar 2017 05:22:11 -0800 (PST) 13, 52 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 13, 52 -- X-Google-Original-From: MicroscopyListserver-NoReply 13, 52 -- {microscopylistserver-noreply-at-microscopy.com} 13, 52 -- Subject: viaWWW:Do you know about these electron dese bodies in ER? 13, 52 -- References: {201703061942.v26JgGmd003198-at-microscopy.com} 13, 52 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 13, 52 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 13, 52 -- X-Forwarded-Message-Id: {201703061942.v26JgGmd003198-at-microscopy.com} 13, 52 -- Message-ID: {8b1b7825-1f60-2a28-aaff-80fde9af9ff4-at-microscopy.com} 13, 52 -- Date: Tue, 7 Mar 2017 07:22:09 -0600 13, 52 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:45.0) 13, 52 -- Gecko/20100101 Thunderbird/45.7.1 13, 52 -- MIME-Version: 1.0 13, 52 -- In-Reply-To: {201703061942.v26JgGmd003198-at-microscopy.com} 13, 52 -- Content-Type: text/plain; charset=windows-1252; format=flowed 13, 52 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
We use a Leica EM UC6 for 12 years now, it is solid as a rock and precise like a swiss clock. I just had to put one drop of oil in the gears mechanism after 10 years (3 screws to unscrew, I was able to do it myself!), which I accomplished very bravely :-D Now I am troubled because I don't know anymore if the most loyal companion of men is the dog or a Leica ultramicrotome!
Regards, Stephane
-------------------------------------------- On Wed, 3/1/17, microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} wrote:
Subject: [Microscopy] viaWWW:Ultramicrotomes and gridstainers To: nizets2-at-yahoo.com Date: Wednesday, March 1, 2017, 1:16 PM
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html ----------------------------------------------------------------------------
X-from: allan.mitchell-at-otago.ac.nz
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both allan.mitchell-at-otago.ac.nz as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: allan.mitchell-at-otago.ac.nz Name: Allan Mitchell
Organization: Otago centre for Electron Microscopy, University of Otago
Title-Subject: [Filtered] Ultramicrotomes and gridstainers
Message: We are in the process of trying to obtain funding to replace one of our ultramicrotomes (Ultracut E) and our aged LKB Ultrostainer. We are looking at - RMC Boeckeler ultramicrotme PT-PCZ and the RMC Boeckeler QG-3100 TEM Stainer, or
- Leica Ultracut EM UC7 ultramicrotome and Leica EM AC20 grid-stainer.
I would be keen to hear of peoples experiences with any of these instruments, positives /negatives.
Many thanks
Allan
Login Host: 139.80.40.139 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
We are in process of decommissioning our JEOL 6100 ( 25 yo), and it will be removed from lab in two weeks to make room for another equipment. The tool was last used over a year ago in good working condition, and had been under PM contract since new. It has an Oxford EDS (ISIS) unit, IR chamber camera, and specimen current meter on it. Please contact me if you are interested in taking it as a whole, or parts of it. You will need to pay for the packing and shipping of the tool from Boston to your location. Thanks.
Anlee Krupp Laboratory Manager Precision Measurement Laboratory Boston University Photonics Center 8 Saint Mary's Street, Boston, MA 02215 Phone: (617) 353-9044 Email: ahk-at-bu.edu
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both lon.nelson-at-leica-microsystems.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: lon.nelson-at-leica-microsystems.com Name: LON NELSON
Leica Microsystems and BitesizeBio have organized a free web-seminar in March that may be of interest to you:
Revealing Cellular Dynamics with Millisecond Precision The New Tool That Turned Electron Micrographs in Motion Picture of Neural Communication
Presenters: Dr. Shigeki Watanabe Johns Hopkins School of Medicine
Dr. Frédéric Leroux Leica Microsystems
What if you can dissect the cellular dynamics with millisecond precision? What if you can unravel the morphological transformation of a neuron millisecond by millisecond using electron microscopy? Could this be even possible?
In this webinar, we will talk about how optogenetics coupled with high-pressure freezing can make this possible. We will discuss how to implement electrical stimulation and why it is superior to light stimulation. We will also discuss the importance of sample processing and the challenges you would face while freezing different types of samples.
Very best regards,
Lon Nelson Director of Sales Microscopy lon.nelson-at-leica-microsystems.com M +1 224-628-2467 | F +1 847-607-3160 Leica Microsystems, Inc. 1700 Leider Ln | Buffalo Grove, IL 60089 (USA)
Login Host: 76.231.70.70 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v2AF5MYb005370 for {MicroscopyListserverArchive6-at-microscopy.com} ; Fri, 10 Mar 2017 09:05:22 -0600 Received: (from mail-at-localhost) by microscopy.com (8.12.11.20060308/8.12.11/Submit) id v2AF5MIU005368; Fri, 10 Mar 2017 09:05:22 -0600
Dear Listers, thanks to all of you who answered my question. LaB6 emitters are unanimously considered stable, yet some of you pointed out that the filament has to be kept heated in order to assure a stable emission, even when not working, e.g. overnight.
I'd like to figure out how common is this approach, and the life of LaB6 emitters operated in this way. It would be very interesting and useful to know your experience also about this point, if you want to share it. Thanks in advance Regards
Compare FEI 12T and FEI Quanta 400 ESEM, both tungsten emitters (exchangeable). Quanta 400 ESEM Average life = 60-80 hr When Quanta vented to change specimen, entire column is vented with AIR (could be better with N2, but not often) Tecnai 12T (120kV) Average life = 1000 hr** When change specimen (single tilt) current condition of filament is maintained until vacuum in specimen chamber has recovered.
Quanta filament is OFF overnight. Tecnai filament is under low current and minimum vacuum pressure 24/7 (10-8 with no break in vacuum in life).
Hope this helps too.
Fred Monson
Frederick C. Monson, PhD Technical Director Center for Microanalysis and Imaging, Research and Training (CMIRT) West Chester University of PA Geology-Astronomy 750 South Church St. West Chester, PA, 19383 fmonson-at-wcupa.edu 610-738-0437(Work)
-----Original Message----- X-from: dcristofori-at-unive.it [mailto:dcristofori-at-unive.it] Sent: Friday, March 10, 2017 10:18 AM To: Monson, Frederick {FMonson-at-wcupa.edu}
Dear Listers, thanks to all of you who answered my question. LaB6 emitters are unanimously considered stable, yet some of you pointed out that the filament has to be kept heated in order to assure a stable emission, even when not working, e.g. overnight.
I'd like to figure out how common is this approach, and the life of LaB6 emitters operated in this way. It would be very interesting and useful to know your experience also about this point, if you want to share it. Thanks in advance Regards
LaB6 emitters, like any other source, outgas when first heated and may then be unstable. A way of getting round this is to run the filament at a very low current, just to keep it warm, even if the instrument is not being used. I do not know if they do it now, but JEOL with LaB6 systems set the filament heating at half value when you "turned it off". At very low emission there is no source evaporation, so the filament life does not suffer.
Steve
Steve Chapman FRMS Protrain for Consultancy and Training in Electron Microscopy +44 (0)7711 606967 web www.emcourses.com
-----Original Message----- X-from: dcristofori-at-unive.it [mailto:dcristofori-at-unive.it] Sent: 10 March 2017 15:07 To: protrain-at-emcourses.com
X-from: PSMnews-at-phillyscope.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both PSMnews-at-phillyscope.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Message: Joint PSM / Drexel Bossone Research Center Symposium on Advances in Microscopy
Wednesday, March 29 Please see the Events Registration page at http://phillyscope.com/events/ to register for the Symposium.
The Philadelphia Society for Microscopy will hold a joint all-day symposium on microscopy with the Drexel University Bossone Research Center in Philadelphia on March 29, 2017. Six talks will be presented on applications of microscopy in both physical and biological subjects. The keynote talks will be given by Jay Jerome of Vanderbilt University and Eric Wieschaus of Princeton University (1995 Nobel Prize in Physiology and Medicine). Jay Jerome will speak on A Microscopists View of Atherosclerosis and Cellular Cholesterol Metabolism. Eric Wieschaus will speak on Light Sheet Imaging of Global Tissue Flows in Living Drosophila Embryos. The entire program is available on http://phillyscope.com/symposium-march-29th/
On-site Registration and check-in will begin at 8:00 am with the talks beginning at 8:40 am. Representatives of many microscopy related manufacturers will be on-hand to discuss latest developments in microscopes and accessories.
The registration fee is $25. This fee also includes PSM membership for 2017. Full time students can register for the reduced cost of $5.00. We have space for approximately 25 student posters. Students who present posters can attend the symposium at no cost. Please feel free to contact us using the Contact page (http://phillyscope.com/contact/) with further questions about posters.
Login Host: 152.51.48.1 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
From glenshan72-at-gmail.com Tue Mar 14 16:34:56 2017 Return-Path: {glenshan72-at-gmail.com} Received: from gmail.com ([211.40.127.194]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v2ELYrV6003999 for {microscopylistserverarchive6-at-microscopy.com} ; Tue, 14 Mar 2017 16:34:55 -0500 Message-ID: {5A0DE88C.98553735-at-gmail.com}
Have not got any messages from the list for a while. Just testing.
==============================Original Headers============================== 1, 33 -- From Aleksandr.Mironov-at-manchester.ac.uk Wed Mar 15 05:04:48 2017 1, 33 -- Received: from probity.mcc.ac.uk (probity.mcc.ac.uk [130.88.200.94]) 1, 33 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v2FA4lU1031784 1, 33 -- for {Microscopy-at-microscopy.com} ; Wed, 15 Mar 2017 05:04:48 -0500 1, 33 -- Received: from asmtp1.its.manchester.ac.uk ([130.88.13.149]) 1, 33 -- by probity.mcc.ac.uk with esmtps (TLSv1:DHE-RSA-AES256-SHA:256) 1, 33 -- (Exim 4.85 (FreeBSD)) 1, 33 -- (envelope-from {Aleksandr.Mironov-at-manchester.ac.uk} ) 1, 33 -- id 1co5vK-000Mfw-Mh 1, 33 -- for Microscopy-at-microscopy.com; Wed, 15 Mar 2017 10:12:54 +0000 1, 33 -- Received: from l-u-mid1527am.it.manchester.ac.uk ([130.88.210.224]:55477) 1, 33 -- by asmtp1.its.manchester.ac.uk with esmtpsa (TLSv1.2:ECDHE-RSA-AES128-GCM-SHA256:128) 1, 33 -- (Exim 4.88) 1, 33 -- (envelope-from {Aleksandr.Mironov-at-manchester.ac.uk} ) 1, 33 -- id 1co5vK-0001Hf-BR 1, 33 -- for Microscopy-at-microscopy.com; Wed, 15 Mar 2017 10:12:54 +0000 1, 33 -- To: Microscopy-at-microscopy.com 1, 33 -- From: Aleksandr Mironov {Aleksandr.Mironov-at-manchester.ac.uk} 1, 33 -- Subject: Test message 1, 33 -- Message-ID: {93c79c9a-1327-8a1f-50b3-36e3af19ae8f-at-manchester.ac.uk} 1, 33 -- Date: Wed, 15 Mar 2017 10:12:54 +0000 1, 33 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:45.0) Gecko/20100101 1, 33 -- Thunderbird/45.8.0 1, 33 -- MIME-Version: 1.0 1, 33 -- Content-Type: text/plain; charset=utf-8; format=flowed 1, 33 -- Content-Transfer-Encoding: 7bit 1, 33 -- X-Authenticated-Sender: Aleksandr Mironov from l-u-mid1527am.it.manchester.ac.uk [130.88.210.224]:55477 1, 33 -- X-Authenticated-From: Aleksandr.Mironov-at-manchester.ac.uk 1, 33 -- X-SA-Exim-Connect-IP: 130.88.13.149 1, 33 -- X-SA-Exim-Mail-From: Aleksandr.Mironov-at-manchester.ac.uk 1, 33 -- X-SA-Exim-Scanned: No (on probity.mcc.ac.uk); SAEximRunCond expanded to false 1, 33 -- X-Spam-Score: -5.0(?) 1, 33 -- X-Spam-Flag: NO ==============================End of - Headers==============================
From voraisai2-at-gmail.com Thu Mar 16 13:04:03 2017 Return-Path: {voraisai2-at-gmail.com} Received: from gmail.com (lync-mediation.hosted.chinatelecomglobal.com [203.14.188.88]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v2GI401w031709 for {microscopylistserverarchive6-at-microscopy.com} ; Thu, 16 Mar 2017 13:04:02 -0500 Message-ID: {91004185.C02BA2C8-at-gmail.com}
Does anybody have floppies from an old Leo 982 Gemini column? I just got one in the shop with the hard drive removed, and have to restore the on-board computer. (If you’ve got an old parts Leo 982 and have a spare drive with the software installed, and would be willing to part with it, that would work, too…)
Thank you,
Justin A. Kraft
==============================Original Headers============================== 3, 46 -- From kraftpiano-at-gmail.com Fri Mar 17 10:27:37 2017 3, 46 -- Received: from mail-qt0-f193.google.com (mail-qt0-f193.google.com [209.85.216.193]) 3, 46 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v2HFRb3F023385 3, 46 -- for {microscopy-at-microscopy.com} ; Fri, 17 Mar 2017 10:27:37 -0500 3, 46 -- Received: by mail-qt0-f193.google.com with SMTP id r5so9925634qtb.2 3, 46 -- for {microscopy-at-microscopy.com} ; Fri, 17 Mar 2017 08:35:53 -0700 (PDT) 3, 46 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 46 -- d=gmail.com; s=20161025; 3, 46 -- h=from:content-transfer-encoding:mime-version:subject:message-id:date 3, 46 -- :to; 3, 46 -- bh=qkfLwJfIw58uMhh8NFiux8SifG2wuY+S5XPQoYhimbg=; 3, 46 -- b=amRPYAhKx7VcwybjRHyuiL9cBE3j7NiBKY53eRUerL8zwPmD89rtCIwqIhLyNn7NQJ 3, 46 -- w3VzYGjDkisKPrsPq1VJL65eAxOpbk/KmMrliPArf7SYJSV55kfDW3hPXaQgmk6Qz+Qg 3, 46 -- 5HXd93eQfeATjLLUxIgtGFEzbiS53PoEWjpZj3X9urWyjqq7P2FHOWTduOpOM+5DXpiE 3, 46 -- v0bkJQMoC+lpBFywXaBU/KX8s4QOzBZa3er2iXmcL5t6mtcMAhihRgv18L3KvPRVj65J 3, 46 -- YFGNcHB58DR0524QZFRLABjxXkXw1juCrREpXu7LokB22mBBpX7s9pPMtKeI6Ei8u63c 3, 46 -- sw+w== 3, 46 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 46 -- d=1e100.net; s=20161025; 3, 46 -- h=x-gm-message-state:from:content-transfer-encoding:mime-version 3, 46 -- :subject:message-id:date:to; 3, 46 -- bh=qkfLwJfIw58uMhh8NFiux8SifG2wuY+S5XPQoYhimbg=; 3, 46 -- b=DvOIO/y2XSJJ08uU1zRwLWD3KxN8dBckBban7YdXbWN2dsLyTqfHqClwfBixHB945M 3, 46 -- OTFTAqvl1WxOA1+pqN0BsSIBEqFqh4EYNq0RyCg4RNU6kpJV6GWlWA79z8Wz1Y9mtBjZ 3, 46 -- 1JQZv2PhNRgldecW4N7NMfdLlFfm2pkVV7KWsvO2bSD6G/DUaLe2BDjeHwnzMJl+LSLE 3, 46 -- fw9Ki8M5MPBK5ewjbecixdYTDxP0gpZu4widPa+0qn5eKw+ZJJkMMfLpqvhxRbhP1ecR 3, 46 -- 7HL0madFiK4jAWq0K4Ujs+IH5PMp8968PLz9qLqsWc7ZFLE61ukMwg/A/vh3GZoNky65 3, 46 -- lnPg== 3, 46 -- X-Gm-Message-State: AFeK/H00Gd4B5pb5bRN1tO+T5O6gYD6i3Rvk8He0y1PVPnVxzR2lIe1JaeHXWt0OhltOoA== 3, 46 -- X-Received: by 10.237.57.169 with SMTP id m38mr15860054qte.196.1489764952990; 3, 46 -- Fri, 17 Mar 2017 08:35:52 -0700 (PDT) 3, 46 -- Received: from justin.jkraft.net (rrcs-208-125-225-220.nys.biz.rr.com. [208.125.225.220]) 3, 46 -- by smtp.gmail.com with ESMTPSA id k36sm6172845qtc.10.2017.03.17.08.35.52 3, 46 -- for {microscopy-at-microscopy.com} 3, 46 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 3, 46 -- Fri, 17 Mar 2017 08:35:52 -0700 (PDT) 3, 46 -- From: Justin Kraft {kraftpiano-at-gmail.com} 3, 46 -- Content-Type: text/plain; charset=utf-8 3, 46 -- Mime-Version: 1.0 (Mac OS X Mail 10.2 \(3259\)) 3, 46 -- Subject: Leo 982 software? 3, 46 -- Message-Id: {E37C8F8C-C379-4B1B-8459-9126324F67BE-at-gmail.com} 3, 46 -- Date: Fri, 17 Mar 2017 11:35:51 -0400 3, 46 -- To: microscopy-at-microscopy.com 3, 46 -- X-Mailer: Apple Mail (2.3259) 3, 46 -- Content-Transfer-Encoding: 8bit 3, 46 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v2HFRb3F023385 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both pscallio-at-dal.ca as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: pscallio-at-dal.ca Name: Pat Scallion
Organization: Institute for Research in Materials Dalhousie University
Title-Subject: [Filtered] S4700 column hot after a power outage
Message: Hi, I have seen odd behavior from the Hitachi S4700, after a power outage. When I arrived at work, the front instrument panel was showing only symbols, not numbers. Also, in addition to other usual things, the column was very hot, and the metal ticking, like what is heard during a bakeout.
I cannot get any image from the upper, TTL detector, and the beam is showing instability, with flashes of brightness and image jumping when in use. The Vext is slower to rise as well, and the IP3 starts at 1x10 -8, then rises to 1x10 -7 while the beam is on. The typical vacuum level for IP3 has been 1x10 -6.
I can send more information if specifics are needed.
Hope someone can help me.
Thanks, Pat Scallion
Login Host: 129.173.116.16 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
Microscopy-at-Microscopy.com
============================================
==============================Original Headers============================== 16, 52 -- From microscopy.listserver-at-gmail.com Sat Mar 18 08:26:42 2017 16, 52 -- Received: from mail-it0-f47.google.com (mail-it0-f47.google.com [209.85.214.47]) 16, 52 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v2IDQgcb000380 16, 52 -- for {microscopy-at-microscopy.com} ; Sat, 18 Mar 2017 08:26:42 -0500 16, 52 -- Received: by mail-it0-f47.google.com with SMTP id m27so55418247iti.1 16, 52 -- for {microscopy-at-microscopy.com} ; Sat, 18 Mar 2017 06:35:02 -0700 (PDT) 16, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 16, 52 -- d=gmail.com; s=20161025; 16, 52 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 16, 52 -- :mime-version:in-reply-to:content-transfer-encoding; 16, 52 -- bh=zDD3hqIEBYzm5IWvpVmfQ4WUuBscXikkHcEc+jgcf80=; 16, 52 -- b=cre8UddTUCKZ5ODlBo/6rDEnj9//oJRwbSS/ZPtnpFrq1E/0isEsBC2ogbtPyvDaDs 16, 52 -- kokF3qCDqlAIN2yKuDA0tu/3U8Noj+Ofmwmzju3/EDEbQs8jbsER2er/VI6zirxWQb0S 16, 52 -- ajBsvPw1MRGJthzOPIYdLSd3MMQ6NeNzDeN4H3auEeFEJVIRxVnkfy/DGJ6gi370yViA 16, 52 -- H2DOwWA1lJESJPYWRl1Hs0ThUPBvNlS5V/dnKfC0s1lQ+WJUpgFuaHNQU7Hbt/ZRHTJa 16, 52 -- fyxnY0pI/zqAFG98rbv3NpHAjAV4OZj8FPm/9BTh4H97aQAoL8BeNQ2NCFQXAS2BfNNw 16, 52 -- OpSA== 16, 52 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 16, 52 -- d=1e100.net; s=20161025; 16, 52 -- h=x-gm-message-state:from:subject:references:to:reply-to:message-id 16, 52 -- :date:user-agent:mime-version:in-reply-to:content-transfer-encoding; 16, 52 -- bh=zDD3hqIEBYzm5IWvpVmfQ4WUuBscXikkHcEc+jgcf80=; 16, 52 -- b=I5rzeF5ZP7BKb92KeFnY3Fkw5/FrCqhwPEvQ8xSzb0Vu2/ksdId0FWR0LZNO68W3el 16, 52 -- te+V+r4ojLWt7CSSXw8lhFtfSkiqnSJaTqBmFHdgQyVRf81LFbhRlXqaN82ZIERUc++7 16, 52 -- K00n3LeqGQigG6/97PXt3eaIkHcgikN2vnR28R7gxmg/Kkz/3o9Rsj7K5q2yfLJ4eU8L 16, 52 -- Bwp7Ev9pyiVJIzEAqgUFYkfv2GY3jdWo7q82bHF2CoGBcFmXQXgjHosbW6l+X4+z0ouQ 16, 52 -- BW1Mii7bxN6b42d2WTECdjnF3Gx3eGAY6oTk6LnEPMUEhl9+z68QZZkIbk5Vk9xRSfAM 16, 52 -- GE9g== 16, 52 -- X-Gm-Message-State: AFeK/H0h1vsTA7sIrPQZStR+Vbi6+dMVUb+wV/jmx+312bZm5oXvYvblUTi3j5OHWl/KDA== 16, 52 -- X-Received: by 10.36.85.148 with SMTP id e142mr3037699itb.106.1489844101481; 16, 52 -- Sat, 18 Mar 2017 06:35:01 -0700 (PDT) 16, 52 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:c8a6:386e:4b4d:2303]) 16, 52 -- by smtp.googlemail.com with ESMTPSA id j14sm4555904itd.0.2017.03.18.06.35.00 16, 52 -- for {microscopy-at-microscopy.com} 16, 52 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 16, 52 -- Sat, 18 Mar 2017 06:35:00 -0700 (PDT) 16, 52 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 16, 52 -- X-Google-Original-From: MicroscopyListserver-NoReply 16, 52 -- {microscopylistserver-noreply-at-microscopy.com} 16, 52 -- Subject: viaWWW:S4700 column hot after a power outage 16, 52 -- References: {201703161602.v2GG23VG027173-at-microscopy.com} 16, 52 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 16, 52 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 16, 52 -- X-Forwarded-Message-Id: {201703161602.v2GG23VG027173-at-microscopy.com} 16, 52 -- Message-ID: {a92e7dc5-33a6-1557-a739-0e8fea2e4fcb-at-microscopy.com} 16, 52 -- Date: Sat, 18 Mar 2017 08:34:59 -0500 16, 52 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:45.0) 16, 52 -- Gecko/20100101 Thunderbird/45.8.0 16, 52 -- MIME-Version: 1.0 16, 52 -- In-Reply-To: {201703161602.v2GG23VG027173-at-microscopy.com} 16, 52 -- Content-Type: text/plain; charset=windows-1252; format=flowed 16, 52 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
From harremme142-at-gmail.com Sun Mar 19 15:03:37 2017 Return-Path: {harremme142-at-gmail.com} Received: from gmail.com (lync.hosted.chinatelecomglobal.com [203.14.188.89]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v2JK3YLe017146 for {microscopylistserverarchive6-at-microscopy.com} ; Sun, 19 Mar 2017 15:03:36 -0500 Message-ID: {FE6C826B.46F47528-at-gmail.com}
Good Morning Allan, I just purchased our second Leica UC7. These are great ultramicrotomes; very compact, easy to use and excellent lighting. I highly recommend them. Greg
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Wednesday, March 1, 2017 7:21 AM To: Hendricks, Gregory {Gregory.Hendricks-at-umassmed.edu}
X-from: allan.mitchell-at-otago.ac.nz
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both allan.mitchell-at-otago.ac.nz as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: allan.mitchell-at-otago.ac.nz Name: Allan Mitchell
Organization: Otago centre for Electron Microscopy, University of Otago
Title-Subject: [Filtered] Ultramicrotomes and gridstainers
Message: We are in the process of trying to obtain funding to replace one of our ultramicrotomes (Ultracut E) and our aged LKB Ultrostainer. We are looking at - RMC Boeckeler ultramicrotme PT-PCZ and the RMC Boeckeler QG-3100 TEM Stainer, or
- Leica Ultracut EM UC7 ultramicrotome and Leica EM AC20 grid-stainer.
I would be keen to hear of peoples experiences with any of these instruments, positives /negatives.
Many thanks
Allan
Login Host: 139.80.40.139 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
Dear Listers, here again to sum up the results of my little survey. It came out that keeping the LaB6 filament heated, even not at a full range, is quite the standard approach for this emitters.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both szou-at-american.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: szou-at-american.edu Name: Shouzhong Zou
Organization: American University
Title-Subject: [Filtered] Cost of adding an e-beam lithography to a JEOL SEM
Message: Hi Everyone. We are interested in adding an e-beam lithography to a JEOL JSM-IT100LA SEM. Do you have any ideas of how much this would cost? We just need a basic EBL to make electrical contact pads for graphene sheets. Thanks.
Login Host: 147.9.74.196 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
Microscopy-at-Microscopy.com
============================================
==============================Original Headers============================== 12, 52 -- From microscopy.listserver-at-gmail.com Tue Mar 21 14:03:32 2017 12, 52 -- Received: from mail-io0-f170.google.com (mail-io0-f170.google.com [209.85.223.170]) 12, 52 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v2LJ3W7i022177 12, 52 -- for {microscopy-at-microscopy.com} ; Tue, 21 Mar 2017 14:03:32 -0500 12, 52 -- Received: by mail-io0-f170.google.com with SMTP id l7so54205766ioe.3 12, 52 -- for {microscopy-at-microscopy.com} ; Tue, 21 Mar 2017 12:12:04 -0700 (PDT) 12, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 12, 52 -- d=gmail.com; s=20161025; 12, 52 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 12, 52 -- :mime-version:in-reply-to:content-transfer-encoding; 12, 52 -- bh=1uLCG/C17hVJnCQiRmmeKgzryGOTdA+arQQkBnnUdpU=; 12, 52 -- b=bu5EUWtbWOlBd6TUTCrLFRTtPcTgBhJFsAItwHTJmpPaaWJHEHZVeFC6PsD3Ieu04w 12, 52 -- QdvUPLK3fOrt5lCA/a+yuP5XsV7CzKoObOn7NjncDO8hMVUMjAMfliid2kCHKP811dW/ 12, 52 -- Pl8sNsspgKBJIADffsUI1bD/dicad0azSLlP7ak1Q8D2rJo+kzR+mGji38v7bxWbOtZH 12, 52 -- NWfoWW/iTSBLVa+kNwkF599/nJYuM91mGkFz8DvRB8hN7tu/POgjhDRkKlpZwURoUkUa 12, 52 -- Fw23A6pT6VKEgB4GprZ0uaJomcVxhw/cWcpFwzoksWu1oOiNnLhN9mPYJ1RYqYJx+t3d 12, 52 -- F4LA== 12, 52 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 12, 52 -- d=1e100.net; s=20161025; 12, 52 -- h=x-gm-message-state:from:subject:references:to:reply-to:message-id 12, 52 -- :date:user-agent:mime-version:in-reply-to:content-transfer-encoding; 12, 52 -- bh=1uLCG/C17hVJnCQiRmmeKgzryGOTdA+arQQkBnnUdpU=; 12, 52 -- b=ZT0SpZg9At1IN98/V5j0Hma2rPzDZ0tQ+JaSu9GcVnYxqyXzCtcEtdpUb9DIQ7s2V3 12, 52 -- N3TldyJc/laYU31KZ4iG5vIHj9+FnQKjwU2HyENKeli3fM9nPAUEVICSWtv4J0AkBOog 12, 52 -- 3MazgFQariU3EoRp5dBz7IJBewSk4wnWbXHnOW+k8Sky1nIi2+oOn8G+0MqgUHmR4cLJ 12, 52 -- QQZ3VwAlMfGj700L6drSxCxIEYDiqYn/zKUT4Ce2Bni2uLLy7xo1E+oh1GKaXi5D5Ehx 12, 52 -- 4Lv/LmO8RvLMCWa1u4YMpppNwUzWejOaVbFVwgkZ2wMYn0nHyLoSV4LQxqc+5sBZs1J1 12, 52 -- scEw== 12, 52 -- X-Gm-Message-State: AFeK/H3vPeSbcADpVTovdraqbHzwD//ogRCjgCdZpSa6at1CbNfm28YhhygW4CZLqJhtXA== 12, 52 -- X-Received: by 10.107.53.88 with SMTP id c85mr10139930ioa.185.1490123523578; 12, 52 -- Tue, 21 Mar 2017 12:12:03 -0700 (PDT) 12, 52 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:2c6d:f7e:efca:a526]) 12, 52 -- by smtp.googlemail.com with ESMTPSA id 62sm9533962itl.1.2017.03.21.12.12.02 12, 52 -- for {microscopy-at-microscopy.com} 12, 52 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 12, 52 -- Tue, 21 Mar 2017 12:12:02 -0700 (PDT) 12, 52 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 12, 52 -- X-Google-Original-From: MicroscopyListserver-NoReply 12, 52 -- {microscopylistserver-noreply-at-microscopy.com} 12, 52 -- Subject: viaWWW: Cost of adding an e-beam lithography to a JEOL SEM 12, 52 -- References: {201703211643.v2LGhrr2016556-at-microscopy.com} 12, 52 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 12, 52 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 12, 52 -- X-Forwarded-Message-Id: {201703211643.v2LGhrr2016556-at-microscopy.com} 12, 52 -- Message-ID: {63723983-eeaf-3d38-fc7b-69ef21b20aca-at-microscopy.com} 12, 52 -- Date: Tue, 21 Mar 2017 14:12:01 -0500 12, 52 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:45.0) 12, 52 -- Gecko/20100101 Thunderbird/45.8.0 12, 52 -- MIME-Version: 1.0 12, 52 -- In-Reply-To: {201703211643.v2LGhrr2016556-at-microscopy.com} 12, 52 -- Content-Type: text/plain; charset=windows-1252; format=flowed 12, 52 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both zhuoli-at-coh.org as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: zhuoli-at-coh.org Name: Zhuo Li
Organization: City of Hope Beckman Research Institute
Title-Subject: [Filtered] Immuno-gold labeled cellular structures on the membrane?
Message: Dear Listers,
We did immunogold labeling on MDA-MB-231 breast cancer cells grown on cover glass to see if the antigen would appear on the cell surface. The cells were fixed in 4% paraformaldehyde and 0.1% glutaraldehyde in 0.1M cacodylate buffer pH7.4. Immunogold labeling was performed before critical point drying and sputter-coating with Au/Pd. Images were taken on a Zeiss Sigma FE-SEM. Besides some scattered signals, a lot of gold particles are localized in one area. An example is here: https://goo.gl/photos/YPXZrwzNAukfsHBw7 Could you tell what the structure it is? Also, there were a lot of gold particles on the background/cover glass? Could you suggest ways of eliminating them.
Thank you in advance!
Zhuo Li
Login Host: 151.152.101.44 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
To evaluate the reliability of any immunolabelling it is required to at least check the negative controls: specimens incubated without primary antibody. Based on those results one can then look at background and what to do about it. If the negative control is clean, you are dealing with labelling based on binding of the primary. Pre-adsorption of the primary can help provide answers as to whether what you observe is specific or non-specific. If the negative control is not clean, the labelling is the result of interaction between gold conjugate and specimen. In that case incubation protocols and specimen conditioning are the first thing to look at.
Bakground, false positives can almost always be controlled. I will be happy to help with detailed suggestions if you would like, but since that might go into products and brands it would be better to do this off-list.
Cheers from sunny New Zealand,
Jan Leunissen Aurion
==============================Original Headers============================== 6, 26 -- From leunissen-at-aurion.nl Thu Mar 23 18:07:29 2017 6, 26 -- Received: from mx1.bbsrv.nl (mx1.bbsrv.nl [84.241.139.140]) 6, 26 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v2NN7TUk024008 6, 26 -- for {microscopy-at-microscopy.com} ; Thu, 23 Mar 2017 18:07:29 -0500 6, 26 -- Received: from localhost (localhost [127.0.0.1]) 6, 26 -- by mx1.bbsrv.nl (Postfix) with ESMTP id 691AF3104; 6, 26 -- Fri, 24 Mar 2017 00:16:05 +0100 (CET) 6, 26 -- Received: from mx1.bbsrv.nl ([127.0.0.1]) 6, 26 -- by localhost (mx1d.bbint [127.0.0.1]) (amavisd-new, port 10024) 6, 26 -- with ESMTP id g5yciCoMvR31; Fri, 24 Mar 2017 00:16:05 +0100 (CET) 6, 26 -- Received: from [192.168.0.5] (122-61-123-118.jetstream.xtra.co.nz [122.61.123.118]) 6, 26 -- by mx1.bbsrv.nl (Postfix) with ESMTPSA id 2D83A30AF; 6, 26 -- Fri, 24 Mar 2017 00:16:03 +0100 (CET) 6, 26 -- Content-Type: text/plain; charset=us-ascii 6, 26 -- Mime-Version: 1.0 (Mac OS X Mail 9.3 \(3124\)) 6, 26 -- Subject: Re: [Microscopy] viaWWW:Immuno-gold labeled cellular structures on the membrane? 6, 26 -- From: Jan Leunissen {leunissen-at-aurion.nl} 6, 26 -- In-Reply-To: {201703232208.v2NM8ibN000943-at-microscopy.com} 6, 26 -- Date: Fri, 24 Mar 2017 12:15:57 +1300 6, 26 -- Cc: zhuoli-at-coh.org 6, 26 -- Message-Id: {651EF269-CDF0-4E8D-87B8-830D4D0D4B9A-at-aurion.nl} 6, 26 -- References: {201703232208.v2NM8ibN000943-at-microscopy.com} 6, 26 -- To: microscopy-at-microscopy.com 6, 26 -- X-Mailer: Apple Mail (2.3124) 6, 26 -- Content-Transfer-Encoding: 8bit 6, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v2NN7TUk024008 ==============================End of - Headers==============================
1. It seems, at least, two possibilities for your picture. One is the structure (with gold particles) could be extracellular matrix, which is usually 'sticky' for gold particles, or could contain the antigen. Another possibility is that it is a broken membrane - you are viewing the inner membrane, which might have more antigen, or being sticky. You may want to do a 'control' with full fixation (Glutaraldehyde + Osmium), which provide you a better resolution of the structure.
2. Aldehyde group, especially that from glutaraldehyde is very 'sticky' to antibodies. Since you are NOT viewing the internal structure, I strongly recommend to avoid using glutaraldehyde, not even 0.1%. It may explain, at least in part, the heavy labeling on your cover glass.
3. Blocking with BSA, and/or serum could reduce the non-specific background.
4. I recommend the sample be coated with carbon, instead of Au/Pd. You can then confirm the gold particles with a backscatter electron detector (here is a reference I published many years ago - Localization of myosin on sperm-cell-associated membranes of tobacco (Nicotiana tabacum L.) - https://link.springer.com/article/10.1007/BF01279082
5. As Jan suggested, control is critical!
Hope this helps and let me know if you need a PDF copy of the reference.
Good Luck,
Zhaojie
Zhaojie Zhang, Ph.D. Director, Jenkins Microscopy Facility University of Wyoming
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Thursday, March 23, 2017 4:14 PM To: Z.J. Zhang {ZZhang-at-uwyo.edu}
X-from: zhuoli-at-coh.org
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both zhuoli-at-coh.org as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: zhuoli-at-coh.org Name: Zhuo Li
Organization: City of Hope Beckman Research Institute
Title-Subject: [Filtered] Immuno-gold labeled cellular structures on the membrane?
Message: Dear Listers,
We did immunogold labeling on MDA-MB-231 breast cancer cells grown on cover glass to see if the antigen would appear on the cell surface. The cells were fixed in 4% paraformaldehyde and 0.1% glutaraldehyde in 0.1M cacodylate buffer pH7.4. Immunogold labeling was performed before critical point drying and sputter-coating with Au/Pd. Images were taken on a Zeiss Sigma FE-SEM. Besides some scattered signals, a lot of gold particles are localized in one area. An example is here: https://goo.gl/photos/YPXZrwzNAukfsHBw7 Could you tell what the structure it is? Also, there were a lot of gold particles on the background/cover glass? Could you suggest ways of eliminating them.
Thank you in advance!
Zhuo Li
Login Host: 151.152.101.44 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
Hi, The Institute of Low Temperature and Structure Research, Polish Academy of Sciences, Wroclaw, Poland has an immediate opening for an Electron Microscopy Technician.
The main responsibilities and duties are: operation and maintenance of FE-SEM (equipped with EDS and EBSD) and ancillary equipment; training and support for users in microscope operation and sample preparation and participation in scientific projects in the field of materials science.
Job requirements: Graduate (B.Sc is a minimum) in physics, material science, chemistry, or similar discipline; experience in operation of SEM and X-ray microanalysis (EDS) (knowledge of EBSD and TEM techniques will be an advantage); good written and oral skills in English.
To view full job posting please visit : http://www.intibs.pl/en/the-institute/news/687
Regards,
Leszek Kepinski
Institute of Low Temperature and Structure Research,
Polish Academy of Sciences,
P.O. Box 1410, 50-950 Wroclaw, Poland
e-mail: L.Kepinski-at-int.pan.wroc.pl
==============================Original Headers============================== 10, 18 -- From l.kepinski-at-int.pan.wroc.pl Fri Mar 24 14:22:48 2017 10, 18 -- Received: from mserv3.int.pan.wroc.pl (mailserver.int.pan.wroc.pl [156.17.85.6]) 10, 18 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v2OJMmT7009132 10, 18 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Mar 2017 14:22:48 -0500 10, 18 -- Received: from poczta.intibs.pl (poczta.int.pan.wroc.pl [156.17.85.10]) 10, 18 -- by mserv3.int.pan.wroc.pl (Postfix) with ESMTPA id 951A9664F7 10, 18 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Mar 2017 20:31:28 +0100 (CET) 10, 18 -- MIME-Version: 1.0 10, 18 -- Content-Type: text/plain; charset=US-ASCII; 10, 18 -- format=flowed 10, 18 -- Content-Transfer-Encoding: 7bit 10, 18 -- Date: Fri, 24 Mar 2017 20:31:28 +0100 10, 18 -- From: "l.kepinski" {l.kepinski-at-int.pan.wroc.pl} 10, 18 -- To: Microscopy-at-microscopy.com 10, 18 -- Subject: Open position SEM 10, 18 -- Message-ID: {6738871a8992cd64e1c03359823477e7-at-int.pan.wroc.pl} 10, 18 -- X-Sender: l.kepinski-at-int.pan.wroc.pl 10, 18 -- User-Agent: Roundcube Webmail/1.0.9 ==============================End of - Headers==============================
The postdoctoral associate is expected to develop and perform TEM characterization of assembly of designed bio-molecular arrays. The research will involve the development of new approaches for cryoEM, cryoET, cryoSTEM, liquidEM characterization of an integration of proteins, peptides and DNA into targeted structures with multiscale organization, and analysis of their structure and assembly processes. The work will be conducted in the Electron Microscopy group in the Center for Functional Nanomaterials which houses five state-of-the-art scanning/ transmission electron microscopes. The majority of the work will be conducted on a FEI Environmental Titan equipped with a K2 direct-electron detector, a FEI Talos F200X, and an aberration-corrected dedicated STEM, first two of which have cryo-transfer, liquid-flow, low-dose, and automated data acquisition capabilities. The successful implementation of the work requires a close interaction with scientists working on bio-inspired self-assembly and advanced characterization methods using x-ray scattering.
Review of applications begins immediately. Applications will be accepted until the position is filled. Research will be under the direction of two well-known electron microscopists at the CFN in close collaboration with PIs and postdocs from the Soft/Bio Group, the Biology Department, and NSLSII.
Required Knowledge, Skills and Abilities:
1. Ph.D. in Physics, Materials Science, Chemistry or Biology 2. Solid background in Transmission Electron Microscopy with rigorous Ph.D. training in a cryoEM or TEM group. 3. Skills in cryoEM/TEM structural characterization at the atomic scale.
Preferred Knowledge, Skills, and Abilities:
Cryo-electron microscopy, single-particle and tomography methods, hands-on experience with TEM sample preparation, such as cryo-plunging and ultramicrotomy
Other Information:
BNL policy states that Research Associate appointments may be made to those who have received their doctoral degrees within the past five years.
The EM facility in the CFN includes 5 transmission electron microscopes, 1 dual-beam FIB, and a collection of specialized holders including liquid and gas flow holders, liquid electrochemical holders, heating holders (single-tilt, double-tilt, and high tilt), cryo-transfer holders (single tilt, double tilt, and high tilt), and nanoindentation holders (single and double tilt).
Interested candidates can apply online by clicking on the link below:
Best Regards, Huolin Xin, Ph.D. Associate Scientist Center for Functional Nanomaterials at Brookhaven National Laboratory and Adjunct Assistant Professor Department of Materials Science and Engineering SUNY Stony Brook University https://sites.google.com/site/xinhuolin/ Email: hxin-at-bnl.gov Office: 631-344-4350
==============================Original Headers============================== 13, 37 -- From xinhuolin-at-gmail.com Fri Mar 24 22:27:47 2017 13, 37 -- Received: from mail-oi0-f52.google.com (mail-oi0-f52.google.com [209.85.218.52]) 13, 37 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v2P3Rlb8010205 13, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Mar 2017 22:27:47 -0500 13, 37 -- Received: by mail-oi0-f52.google.com with SMTP id r203so3475200oib.3 13, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Mar 2017 20:36:31 -0700 (PDT) 13, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 13, 37 -- d=gmail.com; s=20161025; 13, 37 -- h=mime-version:from:date:message-id:subject:to; 13, 37 -- bh=zkfoCNQDFPAbz8HZklpxeEPTFMOtPww2bfwFhkde9TE=; 13, 37 -- b=Uw6xeFR+5MV7Ztr8R0k4ZzxGEwwPGvYcU+oLFWbIotlg1gPrss4CIUz1GIDtxz98Fe 13, 37 -- tWB8ALixRDLxnKR9GsfIogbivI0nXKV168jJlZNutOU63MkPqnt7uEsw8MMpXrMhU42C 13, 37 -- wDHpdBok51F7lZO9aqc38DhZPhU4KZCogkrzW/MhQjQ5dhyZYfF+48HxX1YwcLmGhz91 13, 37 -- EVyf+S2L/dCtMBdz33HX8XCJWX+FJOC8zJ4n8LsFzNc1HR+JRaTOBnBYChzsxCiisKQk 13, 37 -- mqTxoiwbcMwybFW/n2JXhaxasNi1Rww06XJ5O+VWRCnWV/JHVJOYyrRMBa83rIcuzCwd 13, 37 -- bJuQ== 13, 37 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 13, 37 -- d=1e100.net; s=20161025; 13, 37 -- h=x-gm-message-state:mime-version:from:date:message-id:subject:to; 13, 37 -- bh=zkfoCNQDFPAbz8HZklpxeEPTFMOtPww2bfwFhkde9TE=; 13, 37 -- b=QWdWB1SUtNO6L8BDqoEsqUUslhY87RMIhtwuHt5/NTxyL1KvhnoyGF/vvQZw8Mu/0A 13, 37 -- ODkUQMaJaRsfCKS1P7vBrB+ZBR0HL09h0t46KwMp5GoiddUFFdgC+QLXBqPbQQuAJIHE 13, 37 -- IPy8XKc13qf4bpdIsprAOr8pu2656qL3SgKAeLpO4DFgg3b4vc7DGJRw2cBukTkANBWZ 13, 37 -- aDKiKT6d2DkIaMWpXGFmchN9A4klnzdr+p4u2TXQNuKWZtwxnaczFdo/SMjwXp+wTpWC 13, 37 -- e4GcgUhiGKzVNA2EjydIAYanjcnDGV59JofjvM3t6J/Kx/xqPq4WwM6AZ8Fzkf+48uT1 13, 37 -- u4UQ== 13, 37 -- X-Gm-Message-State: AFeK/H11B7DeqOP1jcS7hODpEEvImjRVWpSLiWwBvUl9wQF530dbostZTp1AsZlPap3/Qu7m9Ei689SIEbOcoA== 13, 37 -- X-Received: by 10.202.79.13 with SMTP id d13mr3424oib.1.1490412990497; Fri, 24 13, 37 -- Mar 2017 20:36:30 -0700 (PDT) 13, 37 -- MIME-Version: 1.0 13, 37 -- Received: by 10.74.14.146 with HTTP; Fri, 24 Mar 2017 20:36:10 -0700 (PDT) 13, 37 -- From: Huolin Xin {xinhuolin-at-gmail.com} 13, 37 -- Date: Fri, 24 Mar 2017 22:36:10 -0500 13, 37 -- Message-ID: {CALG6ZvGPWRviXsQ3nE-XBZQ64eX7Ra6jx5Q_XdGXTwXRzf0NsQ-at-mail.gmail.com} 13, 37 -- Subject: CryoEM/TEM Postdoc Position at Brookhaven National Laboratory 13, 37 -- To: Microscopy-at-microscopy.com 13, 37 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
I am looking for a postdoc in the area of STEM and electron tomography. The postdoc will have the opportunity to work with a suite of tools in the Center for Functional Nanomaterials at Brookhaven National Laboratory, including an aberration-corrected FEI Environmental Titan equipped with a K2 direct-electron detector, a FEI Talos F200X equipped with X-FEG, Super-X, and an Enfinium, an aberration-corrected cold-FEG dedicated STEM, and a Nion HERMES (located in Rutgers), first two of which have analytical tomography, cryoET, cryoEM, and liquidEM capabilities. In total, the facility includes 5 transmission electron microscopes, 1 dual-beam FIB, and a collection of specialized holders including liquid and gas flow holders, liquid electrochemical holders, heating holders (single, double, and high tilt), cryogenic holders (single tilt, double tilt, and high tilt), and nanoindentation holders (single and double tilt).
Interested applicants should send a CV, and a one-paragraph description of his/her research/education background. Review of applications begins immediately. Applications will be accepted until the position is filled.
Best Regards, Huolin Xin, Ph.D. Associate Scientist Center for Functional Nanomaterials at Brookhaven National Laboratory and Adjunct Assistant Professor Department of Materials Science and Engineering SUNY Stony Brook University https://sites.google.com/site/xinhuolin/ Email: xinhuolin-at-gmail.com Office: 631-344-4350
==============================Original Headers============================== 4, 37 -- From xinhuolin-at-gmail.com Fri Mar 24 22:32:21 2017 4, 37 -- Received: from mail-ot0-f181.google.com (mail-ot0-f181.google.com [74.125.82.181]) 4, 37 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v2P3WLAK014342 4, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Mar 2017 22:32:21 -0500 4, 37 -- Received: by mail-ot0-f181.google.com with SMTP id a5so4553619oth.1 4, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Mar 2017 20:41:05 -0700 (PDT) 4, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 37 -- d=gmail.com; s=20161025; 4, 37 -- h=mime-version:from:date:message-id:subject:to; 4, 37 -- bh=5Xcxr3NbGwIIN2IbtErTrqEqsNT7Cpoe2qa+2f7w2oI=; 4, 37 -- b=T1MKURD9YeQQjF5bO9Q8+ZAdgMLD9Bo27jeEDoJdLPtacoIxIHpp/hpesfRMBS5vJc 4, 37 -- ROHz23TaFVASZjp+b2/YQVLlXMms6r1PrFrWFUiRuKd9fXoBD48HVsAYfr2xeKLlfND1 4, 37 -- pZ0ls1Y81GNAgD15h/uJGAM38lVjnfx19VuO12hS9aCRfZR7EsD4KgMWrBOb/4QE+sEy 4, 37 -- ySBsjG3EoQC/Pdl+K/x7787ZyhMsLoRc5hxbinKs56R1AneBCFzl+vH0bKDXheeoxvsW 4, 37 -- KV39R/2fgJUz0XibAhOAis69/LrblP/tTkq3ys1JKiHHL8gqnGZx4Dn5rjAh7Da3G5XG 4, 37 -- A87Q== 4, 37 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 37 -- d=1e100.net; s=20161025; 4, 37 -- h=x-gm-message-state:mime-version:from:date:message-id:subject:to; 4, 37 -- bh=5Xcxr3NbGwIIN2IbtErTrqEqsNT7Cpoe2qa+2f7w2oI=; 4, 37 -- b=lGneSSPa9n69oaDgYYXKVeO7B4iQ+bhlhRi/0/6w7uLnf34GxTsV6jhVtunL+zVf3H 4, 37 -- knvUX+3XUkBW/myt/7Oa96g1SdzWIjo7VicOx7KCIlINXkrzd7u+oLNT8TSsNxttz5Qh 4, 37 -- 57n18RnDSdQmO5Q84u6wyjp67ydltElkBfEOUamEPpRG+6Q4s8gESKFjDUVJDFJs/H7r 4, 37 -- RqItgEkM/wCMf68LNwVdeetYS9hVSBgWBVLaZTZBuL5ER0T+anrAG6uZeBdjq8CpXtaQ 4, 37 -- PU4F7bBNuc9RqqK7Cbq3fMJ/5Il4tiMEDa/aSyAX2jxNjsV3vCKBU5n92xt6t5+v5e/3 4, 37 -- 6XHA== 4, 37 -- X-Gm-Message-State: AFeK/H1OEhs4p8fcqT7AekKGWYmxYldJNCE5j16RYLtSabJSUxHqxdtS7qPKOWgJH0sJ45cjaSqPiLNR8Dr91w== 4, 37 -- X-Received: by 10.157.5.1 with SMTP id 1mr6048067otw.162.1490413264651; Fri, 4, 37 -- 24 Mar 2017 20:41:04 -0700 (PDT) 4, 37 -- MIME-Version: 1.0 4, 37 -- Received: by 10.74.14.146 with HTTP; Fri, 24 Mar 2017 20:40:44 -0700 (PDT) 4, 37 -- From: Huolin Xin {xinhuolin-at-gmail.com} 4, 37 -- Date: Fri, 24 Mar 2017 22:40:44 -0500 4, 37 -- Message-ID: {CALG6ZvEosrqdrkGrqa76Zm1eZNRAf3bFPmS7cdZHQtk6NHY45Q-at-mail.gmail.com} 4, 37 -- Subject: STEM/Tomography Postdoc Position at Brookhaven National Laboratory 4, 37 -- To: Microscopy-at-microscopy.com 4, 37 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both zhuoli-at-coh.org as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: zhuoli-at-coh.org Name: Zhuo Li
Organization: City of Hope Beckman Research Institute
Title-Subject: [Filtered] Immuno-gold labeled cellular structures on the membrane?
Message: Dear Listers,
We did immunogold labeling on MDA-MB-231 breast cancer cells grown on cover glass to see if the antigen would appear on the cell surface. The cells were fixed in 4% paraformaldehyde and 0.1% glutaraldehyde in 0.1M cacodylate buffer pH7.4. Immunogold labeling was performed before critical point drying and sputter-coating with Au/Pd. Images were taken on a Zeiss Sigma FE-SEM. Besides some scattered signals, a lot of gold particles are localized in one area. An example is here: https://goo.gl/photos/YPXZrwzNAukfsHBw7 Could you tell what the structure it is? Also, there were a lot of gold particles on the background/cover glass? Could you suggest ways of eliminating them.
Thank you in advance!
Zhuo Li
Login Host: 151.152.101.44 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both anirudha-at-sciencetomorrow.biz as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] The Current Level in ET Detectors After the PRE-AMP stage
Message: Hi,
I have recently started studying SEMs and was looking into the Detector Current value after the Pre-Amplifier stage and before converting it to Digital Voltage.
Can anyone please help me with that
Thank You Anirudha Login Host: 128.163.239.28 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
Microscopy-at-Microscopy.com
============================================
==============================Original Headers============================== 14, 52 -- From microscopy.listserver-at-gmail.com Mon Mar 27 11:45:10 2017 14, 52 -- Received: from mail-it0-f45.google.com (mail-it0-f45.google.com [209.85.214.45]) 14, 52 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v2RGjAdt007089 14, 52 -- for {microscopy-at-microscopy.com} ; Mon, 27 Mar 2017 11:45:10 -0500 14, 52 -- Received: by mail-it0-f45.google.com with SMTP id y18so81754068itc.0 14, 52 -- for {microscopy-at-microscopy.com} ; Mon, 27 Mar 2017 09:54:03 -0700 (PDT) 14, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 14, 52 -- d=gmail.com; s=20161025; 14, 52 -- h=from:subject:references:reply-to:to:message-id:date:user-agent 14, 52 -- :mime-version:in-reply-to:content-transfer-encoding; 14, 52 -- bh=n/9UvBCmNfWzydbj3xBvdKkOYjhzoBiItpKHtIQlqDM=; 14, 52 -- b=C+8QUYzmFZr07ntri7ALxPngIMWslPDby4Gr1u34BnoujBwqhlW1hs80mQkzYBNRT7 14, 52 -- mXXUe0ysZ2fv18LVycD1+Cv3EKhvh+ELha3SadpTrpnwYMD3H6fdiz8ToNEf4L6B3m0c 14, 52 -- uev/xFHVfQtx6Wo9DZIi0U5ibkQiiNm1agrM+RNXzRd7U/mFYhzBJ3HJJOieE8EJFJV3 14, 52 -- h8UEbZ4mCoHGpxyhTxiqGl9vzNg4OPahJ6JuEVy0gBJy//eKd7uR9y31J21bP2APjZbX 14, 52 -- o9aJRU4um+K5iayyeHWlBwOApGzFq+977B8bbXJ5Kq/G3ZJ5Y/Ete75hrdJf4Seg1AkP 14, 52 -- 7X5Q== 14, 52 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 14, 52 -- d=1e100.net; s=20161025; 14, 52 -- h=x-gm-message-state:from:subject:references:reply-to:to:message-id 14, 52 -- :date:user-agent:mime-version:in-reply-to:content-transfer-encoding; 14, 52 -- bh=n/9UvBCmNfWzydbj3xBvdKkOYjhzoBiItpKHtIQlqDM=; 14, 52 -- b=AWm4fGkKt3WBUckliylzSsZFT0UudOi+Q09GGCagfg8IE5fTCkteVkLDkZn2macM1r 14, 52 -- gFTwrPziTurIMIh7kEbOtZU10mMQ1qzMt9r4fZZnA3WzVzrWGH0yzrfIwwTaUMa2GCis 14, 52 -- 0tsopt6bo4L3PNsLjGqsVt+Yy9fiwu3lHotcIHfh7ZwEfOnfMPshkx33sV/IeDlM8sLK 14, 52 -- HlBtmFl8taoPWrQJxNEaY1byrIgi40eel/pCV87tFAOGEuU/foxLO2oEzmgc533sbyHg 14, 52 -- pTYBy2JeLiOAAn+vgj99APxcU55lV6b1n5q0RD7xodbqsPmEZ87bpTHblE2G8+u3Udvu 14, 52 -- QN0A== 14, 52 -- X-Gm-Message-State: AFeK/H0CS1t+a2cZCNOZ7a7ejstozfnRzfcJirM/sPnQ3INP0Qrp/Bav1IgBGPh2lTeF1g== 14, 52 -- X-Received: by 10.107.53.88 with SMTP id c85mr24062931ioa.185.1490633642497; 14, 52 -- Mon, 27 Mar 2017 09:54:02 -0700 (PDT) 14, 52 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:590a:6045:62c8:ca79]) 14, 52 -- by smtp.googlemail.com with ESMTPSA id f127sm64986ite.14.2017.03.27.09.54.01 14, 52 -- for {microscopy-at-microscopy.com} 14, 52 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 14, 52 -- Mon, 27 Mar 2017 09:54:02 -0700 (PDT) 14, 52 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 14, 52 -- X-Google-Original-From: MicroscopyListserver-NoReply 14, 52 -- {microscopylistserver-noreply-at-microscopy.com} 14, 52 -- Subject: viaWWW: The Current Level in ET Detectors After the PRE-AMP stage 14, 52 -- References: {201703271243.v2RChBMN030943-at-microscopy.com} 14, 52 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 52 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 52 -- X-Forwarded-Message-Id: {201703271243.v2RChBMN030943-at-microscopy.com} 14, 52 -- Message-ID: {87e548fa-bc50-a24d-93a5-08c0b8698020-at-microscopy.com} 14, 52 -- Date: Mon, 27 Mar 2017 11:54:01 -0500 14, 52 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:45.0) 14, 52 -- Gecko/20100101 Thunderbird/45.8.0 14, 52 -- MIME-Version: 1.0 14, 52 -- In-Reply-To: {201703271243.v2RChBMN030943-at-microscopy.com} 14, 52 -- Content-Type: text/plain; charset=windows-1252; format=flowed 14, 52 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jotham-at-uchicago.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: jotham-at-uchicago.edu Name: Joe Austin
Organization: The University of Chicago
Title-Subject: [Filtered] EM Technical Director position at University of Chicago
Message: Dear Colleagues,
I would like to call your attention to a Technical Director position in the Advanced Electron Microscopy facility at The University of Chicago (Requisition Number 102322).
For more information about the position and to apply please visit this link: https://jobopportunities.uchicago.edu/applicants/jsp/shared/position/JobDetails_css.jsp?postingId=670183
This position will be open until filled and will be reviewing applications on an ongoing basis. If you have questions about the position please email Joe Austin at jotham-at-uchicago.edu. If you have any difficulty uploading your application or any questions, please email Manuel Carrasquillo at mcarrasquillo-at-bsd.uchicago.edu
The University of Chicago is an Affirmative Action/Equal Opportunity/Disabled/Veterans Employer and does not discriminate on the basis of race, color, religion, sex, sexual orientation, gender identity, national or ethnic origin, age, status as an individual with a disability, protected veteran status, genetic information, or other protected classes under the law. For additional information please see the University's Notice of Nondiscrimination. Staff Job seekers in need of a reasonable accommodation to complete the application process should call 773-834-1841 or email talentacquisition-at-uchicago.edu with their request.
Login Host: 128.135.35.84 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
Microscopy-at-Microscopy.com
============================================
==============================Original Headers============================== 18, 52 -- From microscopy.listserver-at-gmail.com Mon Mar 27 21:51:15 2017 18, 52 -- Received: from mail-it0-f49.google.com (mail-it0-f49.google.com [209.85.214.49]) 18, 52 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v2S2pFql002132 18, 52 -- for {microscopy-at-microscopy.com} ; Mon, 27 Mar 2017 21:51:15 -0500 18, 52 -- Received: by mail-it0-f49.google.com with SMTP id y18so23799597itc.1 18, 52 -- for {microscopy-at-microscopy.com} ; Mon, 27 Mar 2017 20:00:10 -0700 (PDT) 18, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 18, 52 -- d=gmail.com; s=20161025; 18, 52 -- h=from:subject:references:reply-to:to:message-id:date:user-agent 18, 52 -- :mime-version:in-reply-to:content-transfer-encoding; 18, 52 -- bh=GP5yWPfp1n1ruxSvj3nDKIZfAegwc5yJw/mAvnMQGEc=; 18, 52 -- b=gPtiwSqhE0rhLp9+AxmDniPTYDadUTnCDzN/lhuTYvdlZt5UG7ITC5/ZMncnid3OIA 18, 52 -- vbaMs/4ICPn54yWDQAGBwo6tb/LjjFFR0bQzM9ajvRzd2Nx8wWb59SRiSWxHyScjj62s 18, 52 -- xgCxASVB4Rc7h2x6vRLo3LZ2HsrUwPSdtENAeCTPKXJ/B0uxVcMP41/Dem2fNT2Y2icL 18, 52 -- TZdjPHQeGZwmSgogNNHsFVmKpCDZZ6q6SnP2ZzcY0V+9aKsnILjl6hf4ANviZzz21cmA 18, 52 -- qRCLQqLBoAtE7XmnkWaaUkg0ByI50lofdp/zlXgnI1pHCL9JLx6ZdaEhUHIdjacR8K0o 18, 52 -- MRBA== 18, 52 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 18, 52 -- d=1e100.net; s=20161025; 18, 52 -- h=x-gm-message-state:from:subject:references:reply-to:to:message-id 18, 52 -- :date:user-agent:mime-version:in-reply-to:content-transfer-encoding; 18, 52 -- bh=GP5yWPfp1n1ruxSvj3nDKIZfAegwc5yJw/mAvnMQGEc=; 18, 52 -- b=gjlkyDEqDM0zpaNxcEVOsSbMlib/4PDtaTi5TwbA2mQzhxcEnafDu5xV1BIkiDxhLT 18, 52 -- yx1VLZxAEtsDQ4PFOM3k5wKPar+DKpT+UAkfiSgaPcXUcBMStdv2ynjt4td/TMPG3phX 18, 52 -- hBqTNrugj2Y+XHvT2xYeWpdQVxwstzlZhbqakPOU7foyyxrXBZ+wvyIz4W7eWipx/ukJ 18, 52 -- mjK9Q4jE1vIkeZOoFnkvxJz38bL6bMNsNi8fCC9lGIa+lT3+nOyReAR+0WH5yGNs+Xi0 18, 52 -- Y3CYgvlD65QjK1OsfMl2TVjGh/N86J8befMEXjKNf0QWeCgFvIgKFD6TXOn2Qat5RcK8 18, 52 -- O0xw== 18, 52 -- X-Gm-Message-State: AFeK/H3sQ7UwPAE/t+iGxgQx2dp3FsKvegGwB//0p8INk99988gmEbfpNOKGoBTwIJD6Pg== 18, 52 -- X-Received: by 10.36.55.145 with SMTP id r139mr12347824itr.12.1490670009638; 18, 52 -- Mon, 27 Mar 2017 20:00:09 -0700 (PDT) 18, 52 -- Received: from anlvpn001.nst.anl.gov ([130.202.235.1]) 18, 52 -- by smtp.googlemail.com with ESMTPSA id m125sm1250990iom.38.2017.03.27.20.00.08 18, 52 -- for {microscopy-at-microscopy.com} 18, 52 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 18, 52 -- Mon, 27 Mar 2017 20:00:09 -0700 (PDT) 18, 52 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 18, 52 -- X-Google-Original-From: MicroscopyListserver-NoReply 18, 52 -- {microscopylistserver-noreply-at-microscopy.com} 18, 52 -- Subject: viaWWW:EM Technical Director position at University of Chicago 18, 52 -- References: {201703271831.v2RIVvuE020236-at-microscopy.com} 18, 52 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 18, 52 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 18, 52 -- X-Forwarded-Message-Id: {201703271831.v2RIVvuE020236-at-microscopy.com} 18, 52 -- Message-ID: {ff49b7b5-467b-55c0-7bc9-3d2dda212b7b-at-microscopy.com} 18, 52 -- Date: Mon, 27 Mar 2017 22:00:08 -0500 18, 52 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:45.0) 18, 52 -- Gecko/20100101 Thunderbird/45.8.0 18, 52 -- MIME-Version: 1.0 18, 52 -- In-Reply-To: {201703271831.v2RIVvuE020236-at-microscopy.com} 18, 52 -- Content-Type: text/plain; charset=windows-1252; format=flowed 18, 52 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jpshield-at-uga.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: jpshield-at-uga.edu Name: John P Shields
Organization: University of Georgia
Title-Subject: [Filtered] Cathodoluminescence in SEM
Message: We are in the process of adding CL to our FE-SEM. I am not familiar enough with the detectors to know if this is a pain or a positive.
Some questions would be: What is the average lifespan (if there is one) for these detectors? What kind of trouble can you get into with them? Are there any conflicts with other detectors (real or imagined)? How easy is it to use one? Any information that cannot be easily googled would be appreciated.
John S
Login Host: 198.137.20.67 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both j.janssen-at-nki.nl as well as the Microscopy Listserver ---------------------------------------------------------------------------
Message: Does anybody out there have any experience with the Gatan multiple(3)grid cryo holder? We are specifically interested in the grid locking system.
Login Host: 194.171.7.39 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Hasan.Ali-at-angstrom.uu.se as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: Hasan.Ali-at-angstrom.uu.se Name: Hasan Ali
Organization: Uppsala University
Title-Subject: [Filtered] Free Lens Control On JEOL2000FX
Message: Hi,
We have assembled a Gatan energy filter GIF2002 under an old JEOL2000FX. As the GIF doesn't have any communication with the microscope, we don't have a magnification reduction in the microscope when using the GIF and the image/diffraction pattern on the viewing screen is magnified about 19X on the GIF CCD. I want to do angular resolved EELS, but even at the lowest automated camera length (100mm) in the microscope, I cannot get the two beams simultaneously on the CCD for my sample (the collection angle is too low). So I need to go to lower camera lengths using "Free Lens Control" available in the microscpe. I tried Free lens control and obtained the lower camera lengths by changing the strength of intermediate lenses, but when I switch back to image mode, the settings there are changes too and the sample is no more on eucentric height. I don't understand exactly now which lens should I tune in combination with intermediate lenses. Does anyone have the experience of operating the microscope in Free lens control who could suggest me something to follow? Also if someone have the manual to operate the JEOL2000FX in Free Lens Control, that will be very helpful for me. I didn't get information about this in the manuals which I have. Thanks
Login Host: 130.238.23.145 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
From fritschemichael755-at-gmail.com Fri Mar 31 04:20:40 2017 Return-Path: {fritschemichael755-at-gmail.com} Received: from gmail.com (mail.dongnamdc.com [222.97.39.100] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v2V9Kb4Y010736 for {microscopylistserverarchive6-at-microscopy.com} ; Fri, 31 Mar 2017 04:20:39 -0500 Message-ID: {6016F1BB.CABAF2A4-at-gmail.com}
X-from: kmoore-at-vetcor.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both kmoore-at-vetcor.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: kmoore-at-vetcor.com Name: Kasandra Moore
Organization: Geist Station Animal Hospital
Title-Subject: [Filtered] Maintenance Microscope Message: We have a Swift Instrument international SA M3200 microscope. The images are dirty. We think the microscope needs to be cleaned. Can someone provide this service in the Indianapolis area?
Login Host: 108.221.215.45 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
From westelle638-at-gmail.com Sun Apr 2 14:48:15 2017 Return-Path: {westelle638-at-gmail.com} Received: from gmail.com ([221.160.34.117]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v32JmCIC004729 for {microscopylistserverarchive6-at-microscopy.com} ; Sun, 2 Apr 2017 14:48:14 -0500 Message-ID: {AB55A17E.C16BAE9B-at-gmail.com}
The 53rd anniversary meeting of the Southeastern Microscopy Society will be at the Holiday Inn in Athens, GA, on May 24-26, 2017! There is an exciting program planned; Mike Marko of the Wadsworth Center and past president of MSA will be our Invited Speaker. In addition, there will be contributed talks from the members and the Ruska Award student presentations. (Encourage your students to compete in the Ruska Award competition!) A Vendor Social will be held on Wednesday evening, a Banquet on Thursday evening, and a Business Breakfast on Friday morning.
Information is available at http://southeasternmicroscopy.org/2017-2/
Half-day Workshops on Wednesday will include:
RMC and an Array ultramicrotome (at Holiday Inn) FEI and FE-SEM STEM and analysis (at GEM on UGA campus) Negative staining with Sara Miller and Mary Ard (at GEM on UGA campus) Protochips with wet/dry TEM sample holder. (at GEM on UGA campus) Possible Confocal workshop with Zeiss (at the BioImaging Center at UGA)
The abstract deadline is April 14. Please encourage your students to apply for the Ruska Award! http://southeasternmicroscopy.org/ruska-award-student-competition/
The SEMS website is ready for your registration, available at http://southeasternmicroscopy.org/2017-2/ . You can also get to the hotel reservation webpage by clicking on the link on the meeting website.
We hope to see you in Athens!
Terri Bruce (terri-at-clemson.edu), Program Chair John Shields (johnshields-at-gmail.com) and Mary Ard (maryard-at-uga.edu), Local Arrangements Committee
==============================Original Headers============================== 3, 32 -- From ALawrence-at-i2at.msstate.edu Mon Apr 3 12:24:22 2017 3, 32 -- Received: from catalpa.its.msstate.edu (catalpa.its.msstate.edu [130.18.2.119]) 3, 32 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v33HOMEb020873 3, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 3 Apr 2017 12:24:22 -0500 3, 32 -- Received: from mail01.ad.msstate.edu (mail01.ad.msstate.edu [130.18.230.60]) 3, 32 -- by catalpa.its.msstate.edu (8.13.8/8.13.8) with ESMTP id v33HXet3001828 3, 32 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=FAIL) 3, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 3 Apr 2017 12:33:40 -0500 3, 32 -- X-Sender: {} 3, 32 -- Received: from MAIL02.ad.msstate.edu (2620:0:1a30:1230::61) by 3, 32 -- mail01.ad.msstate.edu (2620:0:1a30:1230:85cd:146c:55d4:e430) with Microsoft 3, 32 -- SMTP Server (TLS) id 15.0.1236.3; Mon, 3 Apr 2017 12:33:40 -0500 3, 32 -- Received: from MAIL02.ad.msstate.edu ([fe80::7846:3039:9492:24b0]) by 3, 32 -- mail02.ad.msstate.edu ([fe80::7846:3039:9492:24b0%13]) with mapi id 3, 32 -- 15.00.1236.000; Mon, 3 Apr 2017 12:33:40 -0500 3, 32 -- From: "Lawrence, Amanda" {ALawrence-at-i2at.msstate.edu} 3, 32 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 3, 32 -- Subject: Southeastern Microscopy Meeting, May 24-26 3, 32 -- Thread-Topic: Southeastern Microscopy Meeting, May 24-26 3, 32 -- Thread-Index: AdKsoEJfrTuc/oYFTX6+DsRq0qNfRQ== 3, 32 -- Date: Mon, 3 Apr 2017 17:33:39 +0000 3, 32 -- Message-ID: {80871a69b31449899ee9cb1a087859b3-at-mail02.ad.msstate.edu} 3, 32 -- Accept-Language: en-US 3, 32 -- Content-Language: en-US 3, 32 -- X-MS-Has-Attach: 3, 32 -- X-MS-TNEF-Correlator: 3, 32 -- x-ms-exchange-transport-fromentityheader: Hosted 3, 32 -- x-originating-ip: [130.18.230.93] 3, 32 -- Content-Type: text/plain; charset="iso-8859-1" 3, 32 -- MIME-Version: 1.0 3, 32 -- Content-Transfer-Encoding: 8bit 3, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v33HOMEb020873 ==============================End of - Headers==============================
From operacyjny-at-sezambiuro.pl Mon Apr 3 15:07:31 2017 Return-Path: {operacyjny-at-sezambiuro.pl} Received: from 3580639525.smtp.ogicom.net (3580639525.smtp.ogicom.net [213.108.57.37]) by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v33K7T3k017946; Mon, 3 Apr 2017 15:07:30 -0500 Received: from localhost (localhost.localdomain [127.0.0.1]) by s28-mail.ogicom.net (Postfix) with ESMTP id 598B3A491FD8; Mon, 3 Apr 2017 22:16:47 +0200 (CEST) X-Virus-Scanned: Debian amavisd-new at s28-mail.ogicom.net Received: from s28-mail.ogicom.net ([93.157.100.47]) by localhost (s28-mail.ogicom.net [127.0.0.1]) (amavisd-new, port 10026) with ESMTP id gu4MtyxBOKBp; Mon, 3 Apr 2017 22:16:45 +0200 (CEST) Received: from WS5400D899.PF.local (rrcs-24-172-6-178.midsouth.biz.rr.com [24.172.6.178]) (Authenticated sender: operacyjny.bosezam) by s28-mail.ogicom.net (Postfix) with ESMTPA id 0F014A481449; Mon, 3 Apr 2017 22:16:37 +0200 (CEST) Content-Type: multipart/alternative; boundary="===============1890162325==" MIME-Version: 1.0
X-from: andre.dufresne-at-umanitoba.ca
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both andre.dufresne-at-umanitoba.ca as well as the Microscopy Listserver ---------------------------------------------------------------------------
Message: Hy everyone, I'm looking for a water chiller for a TEM and SEM (H-7000) The unit I'm looking for is a Haskris R075 or equivalent. Options: Water cooled
Message me directly if you have any questions or know the availability of such a unit. Merci /Thank you.
AD
Login Host: 130.179.113.9 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both stacie-at-ems-secure.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] The Aurion Immuno Gold Silver Staining Workshop
Message: Three days of hands-on training for students, researchers, and microscopists who want to learn the most up to date theory and practice in Immuno Gold labeling. Details: Wednesday - Friday May 9-12, 2017 8:00 a.m. - 4:30 p.m. EMS Microscopy Academy Hatfield, Pennsylvania, USA
Instructors: Peter Van De Plas, Aurion, The Netherlands Michael Kostrna, Director, EMS Microscopy Academy Al Coritz, Technical Director, Electron Microscopy Sciences
Class size is limited! For more details, including online registration, visit: http://www.emsdiasum.com/microscopy/academy/courses/immunogold.aspx Login Host: 73.165.127.32 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both gtorraca-at-amgen.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: gtorraca-at-amgen.com Name: Gianni Torraca
Organization: MSA
Title-Subject: [Filtered] Freeze fracture TEM of biologics wanted - service work
Message: Good Day,
I am looking for a lab that can perform freeze fracture analysis of a biologic solution. Kinds regards Gianni Torraca Sr. Scientist Amgen Inc.
Login Host: 198.176.190.243 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
From kimgarr459-at-gmail.com Fri Apr 7 13:02:06 2017 Return-Path: {kimgarr459-at-gmail.com} Received: from gmail.com (59-100-3-189.syd.static-ipl.aapt.com.au [59.100.3.189]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v37I228n024525 for {microscopylistserverarchive6-at-microscopy.com} ; Fri, 7 Apr 2017 13:02:04 -0500 Message-ID: {A014D037.891BAFC7-at-gmail.com}
Bernard J. "Bernie” Kestel the 1994 Microscopy Society of America’s Technologist of the year passed away on April 7.
Bernie was an engineering specialist microscopy technologist for 44 years at Argonne National Laboratory, retiring in 2002. Bernie published numerous articles in microscopy journals and was the recipient of the 1994 Technologist of the Year by the Microscopy Society of America, 1996 Pacesetter Award by Argonne National Laboratory and 1998 Outstanding Service Award by the University of Chicago.
His contributions to the community were in the area of specimen preparation, where he developed new techniques, preparated countless specimens and assisted/trained innumerable students, scientists and colleagues. A compendium of his methodology has been published as a scientific report and is freely available to anyone interested in electropolishing of materials.
Kestel B, (1986) Polishing Methods for Metallic and Ceramic Transmission Electron Microscopy Specimens, ANL-80-120/Rev.1 Report , available from NTIS DE89016686 Issue Number 199005 https://ntrl.ntis.gov/NTRL/dashboard/searchResults.xhtml?searchQuery=ANL-80-120
He was an artist in his field and could always be counted on to find a way to make that critical TEM sample, even when you only had 1small item to work with. He will be remembered here at Argonne and by those he helped in the community, myself included.
=========================================== Dr. Nestor J. Zaluzec Argonne National Laboratory Photon Sciences Division 9700 S. Cass Ave Bldg 212 / A-143 Argonne, Illinois 60439 USA Email: Zaluzec-at-aaem.amc.anl.gov
Tel: 530-NES-TORZ (530-637-8679) has Voice Mail Lab: 630-252-7901 Fax: 630-252-4798
Senior Scientist - Argonne National Laboratory Fellow of the Microscopy Society of America Senior Fellow the Computational Institute - University of Chicago E.P. Wigner Fellow - Oak Ridge National Laboratory Past President Microscopy Society of America Adjunct Professor of Physics - Northern Illinois University & the University of Illinois at Chicago Visiting Professor of Microscopy - Manchester University
*********************************************************************************** Forwarded from "Ask a Microscopist" Please remember that the person asking the question is likely not a member the listserver, and **any reply should go directly to the poster** as well as to the list. Using the "reply" function in your email does *not* send your answer to the person asking the question. Please copy their email address from their question. *********************************************************************************** Name: Richard Gursky School: St. Jude Children’s Research Hospital Grade/Education Level: Graduate Location: Memphis, TN US Email: richard.gursky-at-stjude.org
*********************************************************************************** Forwarded from "Ask a Microscopist" Please remember that the person asking the question is likely not a member the listserver, and **any reply should go directly to the poster** as well as to the list. Using the "reply" function in your email does *not* send your answer to the person asking the question. Please copy their email address from their question. *********************************************************************************** Listers,
This is mostly for those of you on the list who work as applications specialists or service engineers for microscope companies. Perhaps you can send some good advice to Brakel about how to get such a job. Please remember to respond directly to Brakel -- kbrakel91-at-gmail.com
I've already sent some ideas to the Brakel, but since I do not work as an applications person or a service engineer, some pointers from people who do would be more useful.
--------------- Philip Oshel Microscopy Society of America Ask a Microscopist www(dot)microscopy(dot)org/resources/ask(dot)cfm
Name: Kiralyn Brakel School: Texas A&M University Grade/Education Level: Graduate Location: College Station, TX US Email: kbrakel91-at-gmail.com
I'm trying to find the pre-amp pinout designations on an Oxford 7215 Link Pentafet EDS spectrometer. Oxford no longer supports this once highly touted piece of gear and have been unable to help me. Can anyone help with that?
Keeping legacy gear running is made much more difficult by lack of support. I have operated a small electron microprobe lab here in Seattle since 1984 and have useful gear from the entire history of x-ray microanalysis. I'm now using an old ARL SEMQ and a JEOL JXA 8600. I once had the ARL EMX-SM that had the actual moon rocks in it. My computers go all the way back to DEC PDP11s, though that's long gone. The SEMQ still uses MS 6.2. I have thought it might be good to have a used gear forum of some sort. Facebook page ? Wordpress blog ? Any thoughts on that too.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Linda.Davis-at-Vesuvius.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: Linda.Davis-at-Vesuvius.com Name: Linda L Davis
Organization: Vesuvius USA
Title-Subject: [Filtered] celsian or barium feldspar specimens
Message: Hello All,
We have a new chemist in our R&D Analytical Group who needs to do some development work on analyzing barite and celsian using an ICP. I've found nice barite crystals to purchase, but now I need some celsian/barium feldspar. I cannot use eBay at all for work, so I have come to the listserve to see if anyone has any celsian we can purchase or have. I don't need a ton, but numerous crystals/grains to grind up for digestion and analysis. I would greatly appreciate any help.
Sincerely,
Linda L. Davis
Login Host: 199.168.151.35 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both vakimler-at-oakland.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Organization: Oakland University Eye Research Institute
Title-Subject: [Filtered] Whole Cell Mount TEM - Carbon Coating
Message: When one does whole cell mount TEM nowadays on gold-Formvar grids, is it necessary to carbon-coat the samples after preparation (seeding, fixation, staining, dehydration and critical point drying by CO2) or is before the preparation sufficient?
Grids with cells will be stored in a dessicator filled with Drier-Rite.
Thanks, Vickie
Login Host: 141.210.132.220 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
*********************************************************************************** Forwarded from "Ask a Microscopist" Please remember that the person asking the question is likely not a member the listserver, and **any reply should go directly to the poster** as well as to the list. Using the "reply" function in your email does *not* send your answer to the person asking the question. Please copy their email address from their question. ***********************************************************************************
Name: Richard Gursky School: St. Jude Children’s Research Hospital Grade/Education Level: Graduate Location: Memphis, TN US Email: richard.gursky-at-stjude.org
Hello Shouzhong,
I would contact Joe Nabity to see if his Nanometer Pattern Generation System can be added to your 'scope. We use it on our FIB and are happy with the performance. You can find more information here: http://www.jcnabity.com/
I have no ties, financial or otherwise, to Joe or his company.
Thanks, Chris
On Tue, Mar 21, 2017 at 3:15 PM, {microscopy.listserver-at-gmail.com} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: szou-at-american.edu } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } szou-at-american.edu as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: szou-at-american.edu Name: Shouzhong Zou } } Organization: American University } } Title-Subject: [Filtered] Cost of adding an e-beam lithography to a JEOL SEM } } Message: Hi Everyone. We are interested in adding an e-beam lithography to a JEOL JSM-IT100LA SEM. } Do you have any ideas of how much this would cost? We just need a basic EBL to make electrical } contact pads for graphene sheets. Thanks. } } Login Host: 147.9.74.196 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } -- } =========================================== } Do not reply to this message it is from } the Microscopy Listserver NO-REPLY forwarding } system. You should send a new message to } } Microscopy-at-Microscopy.com } } ============================================ } } ==============================Original Headers============================== } 12, 52 -- From microscopy.listserver-at-gmail.com Tue Mar 21 14:03:32 2017 } 12, 52 -- Received: from mail-io0-f170.google.com (mail-io0-f170.google.com [209.85.223.170]) } 12, 52 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v2LJ3W7i022177 } 12, 52 -- for {microscopy-at-microscopy.com} ; Tue, 21 Mar 2017 14:03:32 -0500 } 12, 52 -- Received: by mail-io0-f170.google.com with SMTP id l7so54205766ioe.3 } 12, 52 -- for {microscopy-at-microscopy.com} ; Tue, 21 Mar 2017 12:12:04 -0700 (PDT) } 12, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 12, 52 -- d=gmail.com; s=20161025; } 12, 52 -- h=from:subject:references:to:reply-to:message-id:date:user-agent } 12, 52 -- :mime-version:in-reply-to:content-transfer-encoding; } 12, 52 -- bh=1uLCG/C17hVJnCQiRmmeKgzryGOTdA+arQQkBnnUdpU=; } 12, 52 -- b=bu5EUWtbWOlBd6TUTCrLFRTtPcTgBhJFsAItwHTJmpPaaWJHEHZVeFC6PsD3Ieu04w } 12, 52 -- QdvUPLK3fOrt5lCA/a+yuP5XsV7CzKoObOn7NjncDO8hMVUMjAMfliid2kCHKP811dW/ } 12, 52 -- Pl8sNsspgKBJIADffsUI1bD/dicad0azSLlP7ak1Q8D2rJo+kzR+mGji38v7bxWbOtZH } 12, 52 -- NWfoWW/iTSBLVa+kNwkF599/nJYuM91mGkFz8DvRB8hN7tu/POgjhDRkKlpZwURoUkUa } 12, 52 -- Fw23A6pT6VKEgB4GprZ0uaJomcVxhw/cWcpFwzoksWu1oOiNnLhN9mPYJ1RYqYJx+t3d } 12, 52 -- F4LA== } 12, 52 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 12, 52 -- d=1e100.net; s=20161025; } 12, 52 -- h=x-gm-message-state:from:subject:references:to:reply-to:message-id } 12, 52 -- :date:user-agent:mime-version:in-reply-to:content-transfer-encoding; } 12, 52 -- bh=1uLCG/C17hVJnCQiRmmeKgzryGOTdA+arQQkBnnUdpU=; } 12, 52 -- b=ZT0SpZg9At1IN98/V5j0Hma2rPzDZ0tQ+JaSu9GcVnYxqyXzCtcEtdpUb9DIQ7s2V3 } 12, 52 -- N3TldyJc/laYU31KZ4iG5vIHj9+FnQKjwU2HyENKeli3fM9nPAUEVICSWtv4J0AkBOog } 12, 52 -- 3MazgFQariU3EoRp5dBz7IJBewSk4wnWbXHnOW+k8Sky1nIi2+oOn8G+0MqgUHmR4cLJ } 12, 52 -- QQZ3VwAlMfGj700L6drSxCxIEYDiqYn/zKUT4Ce2Bni2uLLy7xo1E+oh1GKaXi5D5Ehx } 12, 52 -- 4Lv/LmO8RvLMCWa1u4YMpppNwUzWejOaVbFVwgkZ2wMYn0nHyLoSV4LQxqc+5sBZs1J1 } 12, 52 -- scEw== } 12, 52 -- X-Gm-Message-State: AFeK/H3vPeSbcADpVTovdraqbHzwD//ogRCjgCdZpSa6at1CbNfm28YhhygW4CZLqJhtXA== } 12, 52 -- X-Received: by 10.107.53.88 with SMTP id c85mr10139930ioa.185.1490123523578; } 12, 52 -- Tue, 21 Mar 2017 12:12:03 -0700 (PDT) } 12, 52 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:2c6d:f7e:efca:a526]) } 12, 52 -- by smtp.googlemail.com with ESMTPSA id 62sm9533962itl.1.2017.03.21.12.12.02 } 12, 52 -- for {microscopy-at-microscopy.com} } 12, 52 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 12, 52 -- Tue, 21 Mar 2017 12:12:02 -0700 (PDT) } 12, 52 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} } 12, 52 -- X-Google-Original-From: MicroscopyListserver-NoReply } 12, 52 -- {microscopylistserver-noreply-at-microscopy.com} } 12, 52 -- Subject: viaWWW: Cost of adding an e-beam lithography to a JEOL SEM } 12, 52 -- References: {201703211643.v2LGhrr2016556-at-microscopy.com} } 12, 52 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 12, 52 -- Reply-To: microscopylistserver-noreply-at-microscopy.com } 12, 52 -- X-Forwarded-Message-Id: {201703211643.v2LGhrr2016556-at-microscopy.com} } 12, 52 -- Message-ID: {63723983-eeaf-3d38-fc7b-69ef21b20aca-at-microscopy.com} } 12, 52 -- Date: Tue, 21 Mar 2017 14:12:01 -0500 } 12, 52 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:45.0) } 12, 52 -- Gecko/20100101 Thunderbird/45.8.0 } 12, 52 -- MIME-Version: 1.0 } 12, 52 -- In-Reply-To: {201703211643.v2LGhrr2016556-at-microscopy.com} } 12, 52 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 12, 52 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
==============================Original Headers============================== 5, 67 -- From microscopy.listserver-at-gmail.com Fri Apr 14 07:14:34 2017 5, 67 -- Received: from mail-io0-f173.google.com (mail-io0-f173.google.com [209.85.223.173]) 5, 67 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v3ECEXaH026353 5, 67 -- for {microscopy-at-microscopy.com} ; Fri, 14 Apr 2017 07:14:34 -0500 5, 67 -- Received: by mail-io0-f173.google.com with SMTP id l7so108203098ioe.3 5, 67 -- for {microscopy-at-microscopy.com} ; Fri, 14 Apr 2017 05:24:31 -0700 (PDT) 5, 67 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 5, 67 -- d=1e100.net; s=20161025; 5, 67 -- h=x-gm-message-state:resent-from:resent-to:resent-date 5, 67 -- :resent-message-id:resent-user-agent:dkim-signature:mime-version 5, 67 -- :in-reply-to:references:from:date:message-id:subject:to:cc; 5, 67 -- bh=d6xhlXgzRn41mrxxDjdD1urgqZnHk+wQAdSh6NUIycw=; 5, 67 -- b=sVxZFaEFjve6O8wW3YZBIfy2bSrHxvwXCgXYvwdRTTgbJBFA1/mXeSxlkInK8VCViL 5, 67 -- RvCHdqjHVZA4y0pL6HaS8kw2mmCH61OqzR6bUfL9Ph/AYUcn5himpYwqDM5VLxC2VqNY 5, 67 -- k5RhM/2Fkvx1eS3VMc1XAullvV3Q743UFrPv2WHxRQqou/HRCi7HQwoeeSyNcxh4G0CH 5, 67 -- XbMBVT5HJ5i6bNTUTfodpFjmAnUf9PYqqjhCiODex8ZUXvdcGZYGPyyJXwTwF+dNqFa0 5, 67 -- 0fLFZt6R4orebLtwaVN+f4wCfdeGbHxp/L4bITDKxZ2V9fFQx+deVGTMYETqfmtCD4aF 5, 67 -- 61+w== 5, 67 -- X-Gm-Message-State: AN3rC/4PJQwZU1lYqMwmCO6g9jZIlmFzVgNRe7kYNVqoB1wfWvJTuwle 5, 67 -- VEcE/IsQuPF1njbKSEE= 5, 67 -- X-Received: by 10.107.168.90 with SMTP id r87mr9652234ioe.45.1492172670703; 5, 67 -- Fri, 14 Apr 2017 05:24:30 -0700 (PDT) 5, 67 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:a58c:e87a:34da:c5cc]) 5, 67 -- by smtp.googlemail.com with ESMTPSA id 141sm963580ioe.47.2017.04.14.05.24.30 5, 67 -- for {microscopy-at-microscopy.com} 5, 67 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 5, 67 -- Fri, 14 Apr 2017 05:24:30 -0700 (PDT) 5, 67 -- Resent-From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} 5, 67 -- Resent-To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 5, 67 -- Resent-Date: Fri, 14 Apr 2017 07:24:29 -0500 5, 67 -- Resent-Message-ID: {39e60e24-f5c9-c544-9248-c29d34bf5993-at-gmail.com} 5, 67 -- Resent-User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:45.0) Gecko/20100101 Thunderbird/45.8.0 5, 67 -- Received: by 10.103.138.8 with SMTP id m8csp662021vsd; 5, 67 -- Tue, 21 Mar 2017 12:28:40 -0700 (PDT) 5, 67 -- X-Received: by 10.200.41.33 with SMTP id y30mr32620624qty.47.1490124520050; 5, 67 -- Tue, 21 Mar 2017 12:28:40 -0700 (PDT) 5, 67 -- Received: from mail-sor-f41.google.com (mail-sor-f41.google.com. [209.85.220.41]) 5, 67 -- by mx.google.com with SMTPS id a6sor872005qta.18.1969.12.31.16.00.00 5, 67 -- for {microscopy.listserver-at-gmail.com} 5, 67 -- (Google Transport Security); 5, 67 -- Tue, 21 Mar 2017 12:28:40 -0700 (PDT) 5, 67 -- Received-SPF: pass (google.com: domain of microwink-at-gmail.com designates 209.85.220.41 as permitted sender) client-ip=209.85.220.41; 5, 67 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 5, 67 -- d=gmail.com; s=20161025; 5, 67 -- h=mime-version:in-reply-to:references:from:date:message-id:subject:to 5, 67 -- :cc; 5, 67 -- bh=d6xhlXgzRn41mrxxDjdD1urgqZnHk+wQAdSh6NUIycw=; 5, 67 -- b=IDDmrx7zvlIEsi8lxalhV9QI3ypT3h1jaI9usJa+Ehsirq3Skv75W4a7DhjFucNC8v 5, 67 -- y0oPYEqIjy9es/H3rvVI+HgygqkpJF4KSGEep3c73XDNzjp7Ys8d5vHywD55E3C66J7r 5, 67 -- h/LLYO24rI2UdpXV805mpswcSK/+5LXHdsbUcyjEwLVU7Bz4+QARU5322P8vEx7rYDUw 5, 67 -- wNyA++KqLAktlxQu7kcBvS13lXpXHlm6fZwIN3CP01i8WtjVzFM1P2w/y+kh7YUWKopu 5, 67 -- 7din7bNtlFKy2mDW/VD0yxkVvBcUIMxNY94yaiyQcoleku8TpIfeGL8W37b5kdxdZ0PB 5, 67 -- vqYg== 5, 67 -- X-Received: by 10.200.53.99 with SMTP id z32mr37896060qtb.106.1490124519617; 5, 67 -- Tue, 21 Mar 2017 12:28:39 -0700 (PDT) 5, 67 -- MIME-Version: 1.0 5, 67 -- Received: by 10.12.133.131 with HTTP; Tue, 21 Mar 2017 12:28:39 -0700 (PDT) 5, 67 -- In-Reply-To: {201703211915.v2LJF7Kv031852-at-microscopy.com} 5, 67 -- References: {201703211915.v2LJF7Kv031852-at-microscopy.com} 5, 67 -- From: Christopher Winkler {microwink-at-gmail.com} 5, 67 -- Date: Tue, 21 Mar 2017 15:28:39 -0400 5, 67 -- Message-ID: {CAA8T2PN4nNSuVq_ixTJfKXxQBPVD+9BLJeepd8M5wGbfxppFBg-at-mail.gmail.com} 5, 67 -- Subject: Re: [Microscopy] viaWWW: Cost of adding an e-beam lithography to a 5, 67 -- JEOL SEM 5, 67 -- To: szou-at-american.edu 5, 67 -- Cc: microscopy.listserver-at-gmail.com 5, 67 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
From fritschemichael755-at-gmail.com Sat Apr 15 10:08:54 2017 Return-Path: {fritschemichael755-at-gmail.com} Received: from gmail.com ([182.16.70.114]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v3FF8pL6028002 for {microscopylistserverarchive6-at-microscopy.com} ; Sat, 15 Apr 2017 10:08:53 -0500 Message-ID: {7ED2CF8A.D62BFD55-at-gmail.com}
X-from: mryan-at-cshl.edu
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mryan-at-cshl.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: mryan-at-cshl.edu Name: Marjorie Ryan
Organization: Cold Spring Harbor Laboratory
Title-Subject: [Filtered] Searching for an Electron Microscopy Technologist
Message: Cold Spring Harbor Laboratory seeks a highly motivated dedicated individual to work in a state-of-the-art Microscopy Shared Resource. The individual should have extensive practical expertise in biological sample preparation for transmission and scanning electron microscopy. Hands-on knowledge of confocal and widefield fluorescence microscopy would also be a plus. The candidate will help users design innovative experiments and they will carry out sample preparation and imaging as well as assist in data interpretation. Excellent verbal and written communication skills, ability to work with multiple users in a supporting role, and ability to work independently and proactively with limited supervision are essential. To Apply:
Interested individuals should send their resume, including a description of their practical expertise and the names and email addresses of 3 references to: Marjorie Ryan, Cold Spring Harbor Laboratory, One Bungtown Road, Cold Spring Harbor, New York 11724, email: mryan-at-cshl.edu Cold Spring Harbor Laboratory is a world-renowned research and educational institution recognized internationally for its excellence in ground-breaking research programs in cancer, neuroscience, plant biology, genomics, and bioinformatics and broad educational mission. For more information about CSHL, please visit us at www.cshl.edu CSHL is an EO/AA Employer. All qualified applicants will receive consideration for employment and will not be discriminated against on the basis of race, color, religion, sex, sexual orientation, gender identity, national origin, age, disability or protected veteran status. VEVRAA Federal Contractor
Login Host: 96.65.115.77 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
From wandjoan59-at-gmail.com Mon Apr 17 04:05:20 2017 Return-Path: {wandjoan59-at-gmail.com} Received: from gmail.com ([182.16.74.250]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v3H95Ibu026977 for {microscopylistserverarchive6-at-microscopy.com} ; Mon, 17 Apr 2017 04:05:19 -0500 Message-ID: {0584B3E5.EBD7DE94-at-gmail.com}
X-from: ravi.thakkar369-at-gmail.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ravi.thakkar369-at-gmail.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: ravi.thakkar369-at-gmail.com Name: Ravi
Organization: Ravi Thakkar
Title-Subject: [Filtered] Source of Standard zinc oxide and iron oxide sample for EDS reference.
Message: Hi All, One of the user for our EM facility is looking for Zinc Oxide and Iron Oxide as standard reference for EDS analysis. If any one can help. Thanks in advance. Login Host: 129.130.145.60 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
We may need more information before we can answer the question well - at least, I would need more.
Is this EDS in conjunction with TEM or SEM? The form of the material would different for the two applications. - If TEM, I don't have much to suggest since I do SEM. It seems they would want a thin film or powder. - If SEM, they would probably want a homogenous, bulk sample. They can get bulk samples of iron oxide. Does it matter if it is Fe2O3 or Fe3O4? I am used to zinc oxide being a powder. It might be challenging to find it in bulk form.
What do they ultimately want to know? Do they want to know if their material matches? Do they want to quantify the oxide? Do they want to see if they have excess oxygen? I often find users coming in with too narrow a question. When I found out the true issue, there is usually much more freedom in suggesting a solution.
Why do they want the oxides? Most EDS systems will have standards built-in for the elements. They are often quite good. I wouldn't think that they need the oxides of the metals.
What form is their sample in, bulk, powder, film? If it is not flat, polished, thick material, then accurate quant will be out of the question.
Hopefully the answer is not simply "Because". I find it much easier to help someone who is forthcoming with information rather than one who is dead-set on a single course of action.
Regards, Warren Straszheim
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Monday, April 17, 2017 9:20 PM To: Straszheim, Warren E [BIOTC]
X-from: ravi.thakkar369-at-gmail.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ravi.thakkar369-at-gmail.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: ravi.thakkar369-at-gmail.com Name: Ravi
Organization: Ravi Thakkar
Title-Subject: [Filtered] Source of Standard zinc oxide and iron oxide sample for EDS reference.
Message: Hi All, One of the user for our EM facility is looking for Zinc Oxide and Iron Oxide as standard reference for EDS analysis. If any one can help. Thanks in advance. Login Host: 129.130.145.60 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
I'm struggling with Williams and Carter (2nd edition, I think) understanding HRTEM. I generally find their text approachable and easy to read, but not the HRTEM material, other than the math.
Is their a text or article that is more detailed? Outside of the Supposedly HRTEM sources that I have found have lengthy introductions to the basic, non-HR microscope, then brief descriptions of the math of HRTEM.
What's a good Read?
I'm a microscopist, with a B.S., so technical is okay, but I want a deep focus on HRTEM, theory and instrumentation.
Thanks!
Kleo (Kathleen) Pullin Moraga, CA 209-610-0555 kleopullin-at-email.arizona.edu https://www.linkedin.com/in/kleopullin https://twitter.com/resolvingdust
On 4/17/2017 7:28 PM, microscopy.listserver-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: ravi.thakkar369-at-gmail.com } } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } ravi.thakkar369-at-gmail.com as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: ravi.thakkar369-at-gmail.com Name: Ravi } } Organization: Ravi Thakkar } } Title-Subject: [Filtered] Source of Standard zinc oxide and iron oxide sample for EDS reference. } } Message: Hi All, } One of the user for our EM facility is looking for Zinc Oxide and Iron Oxide as standard reference } for EDS analysis. If any one can help. Thanks in advance. } Login Host: 129.130.145.60 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } }
-- John J. Donovan donovan-at-uoregon.edu University of Oregon (541) 346-4632 (office) 1443 E. 13th Ave (541) 346-4655 (probe) Eugene, OR (541) 346-6854 (FAX) 97403-1241
On Tue, Apr 18, 2017 at 7:17 AM, {wesaia-at-iastate.edu {mailto:wesaia-at-iastate.edu} } wrote:
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver On-Line {http://www.microscopy.com/MicroscopyListserver%0AOn-Line} Help http://www.microscopy.com/MicroscopyListserver/FAQ.html {http://www.microscopy.com/MicroscopyListserver/FAQ.html} ----------------------------------------------------------------------------
We may need more information before we can answer the question well - at least, I would need more.
Is this EDS in conjunction with TEM or SEM? The form of the material would different for the two applications. - If TEM, I don't have much to suggest since I do SEM. It seems they would want a thin film or powder. - If SEM, they would probably want a homogenous, bulk sample. They can get bulk samples of iron oxide. Does it matter if it is Fe2O3 or Fe3O4? I am used to zinc oxide being a powder. It might be challenging to find it in bulk form.
What do they ultimately want to know? Do they want to know if their material matches? Do they want to quantify the oxide? Do they want to see if they have excess oxygen? I often find users coming in with too narrow a question. When I found out the true issue, there is usually much more freedom in suggesting a solution.
Why do they want the oxides? Most EDS systems will have standards built-in for the elements. They are often quite good. I wouldn't think that they need the oxides of the metals.
What form is their sample in, bulk, powder, film? If it is not flat, polished, thick material, then accurate quant will be out of the question.
Hopefully the answer is not simply "Because". I find it much easier to help someone who is forthcoming with information rather than one who is dead-set on a single course of action.
Regards, Warren Straszheim
-----Original Message----- X-from: microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} [mailto:microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} ] Sent: Monday, April 17, 2017 9:20 PM To: Straszheim, Warren E [BIOTC] Subject: [Microscopy] viaWWW:Source of Standard zinc oxide and iron oxide sample for EDS
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver On-Line {http://www.microscopy.com/MicroscopyListserver%0AOn-Line} Help http://www.microscopy.com/MicroscopyListserver/FAQ.html {http://www.microscopy.com/MicroscopyListserver/FAQ.html} ----------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html {http://microscopy.com/MLFormMail.html} --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ravi.thakkar369-at-gmail.com {mailto:ravi.thakkar369-at-gmail.com} as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: ravi.thakkar369-at-gmail.com {mailto:ravi.thakkar369-at-gmail.com} Name: Ravi
Organization: Ravi Thakkar
Title-Subject: [Filtered] Source of Standard zinc oxide and iron oxide sample for EDS reference.
Message: Hi All, One of the user for our EM facility is looking for Zinc Oxide and Iron Oxide as standard reference for EDS analysis. If any one can help. Thanks in advance. Login Host: 129.130.145.60 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both michael.cammer-at-med.nyu.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: michael.cammer-at-med.nyu.edu Name: Michael Cammer
Organization: NYULMC
Title-Subject: [Filtered] need 700-800 nm excitation for Zeiss AxioObserver
Message: We need to excite in the 700 to 800 nm range and the Zeiss HPX-120 lamp we have is filtered to block wavelengths above 660 nm. Rather than customize the filter inside the lamp house, we are looking for an alternative light source for the 700-800 nm range with a liquid light guide or fiber that we could swap for the Zeiss HPX-120 on the days we need to image infra-red.
Are there any simple light sources that fit this description that cost less than $3k? If not, how about ones that dont cost much more than this?
Thank you!
Login Host: 216.165.126.103 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
I'd say it depends on how stable you want the line (frequency) to be.
CD-ROM lasers are 780nm, and super cheap thanks to economies of scale. Getting one coupled to the fiber of your choice would then be your challenge.
On Tue, Apr 18, 2017 at 6:08 PM, {microscopy.listserver-at-gmail.com} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: michael.cammer-at-med.nyu.edu } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } michael.cammer-at-med.nyu.edu as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: michael.cammer-at-med.nyu.edu Name: Michael Cammer } } Organization: NYULMC } } Title-Subject: [Filtered] need 700-800 nm excitation for Zeiss AxioObserver } } Message: We need to excite in the 700 to 800 nm range and the Zeiss HPX-120 lamp we have is filtered } to block wavelengths above 660 nm. Rather than customize the filter inside the lamp house, we are } looking for an alternative light source for the 700-800 nm range with a liquid light guide or fiber } that we could swap for the Zeiss HPX-120 on the days we need to image infra-red. } } Are there any simple light sources that fit this description that cost less than $3k? If not, how } about ones that don’t cost much more than this? } } Thank you!
*********************************************************************************** Forwarded from "Ask a Microscopist" Please remember that the person asking the question is likely not a member the listserver, and **any reply should go directly to the poster** as well as to the list. Using the "reply" function in your email does *not* send your answer to the person asking the question. Please copy their email address from their question. ***********************************************************************************
It should be possible to import .spx file into this software and then export the data in cvs format. Unfortunately I do not have any spx file at hand, but I have tested it on msa and spc files and it works well.
Best regards from Prague
Oldrich
-- Oldřich Benada Institute of Microbiology CAS, v.v.i. Laboratory of Molecular Structure Characterization Vídeňská 1083 142 20 Prague 4 Czech Republic
On Wed, 19 Apr 2017 06:59:11 -0500, oshel1pe-at-cmich.edu wrote : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } *********************************************************************************** } Forwarded from "Ask a Microscopist" } Please remember that the person asking the question is likely } not a member the listserver, and } **any reply should go directly to the poster** } as well as to the list. } Using the "reply" function in your email does *not* send your answer } to the person asking the question. } Please copy their email address from their question. } *********************************************************************************** } } -----Original Message----- } X-from: "AssociationManagement-at-microscopy.org" } {associationmanagement-at-microscopy.org} Date: Wednesday, 19April, } 2017 at 01:08 To: "AssociationManagement-at-microscopy.org" } {associationmanagement-at-microscopy.org} , Philip Oshel } {oshel1pe-at-cmich.edu} Subject: Ask a Microscopist } } Name:Anuja Bhalkikar } School:University of Nebraska Lincoln } Grade/Education Level:Graduate } Location:Lincoln, NE } Email:anuja.bhalkikar-at-huskers.unl.edu } Subject:Convert .spx data to .txt or .csvYour } Question:Hello, I recently used an FEI Tecnai Osiris S/TEM to obtain } EDS of my sample. I believe we have a Super-X EDX detection system in } conjunction with Bruker Esprit software. The data was unfortunately } saved as .spx. Is there any way I could convert it into .txt or .csv } format? Thanks, Anuja } } } } ==============================Original } Headers============================== 5, 52 -- From } oshel1pe-at-cmich.edu Wed Apr 19 06:56:00 2017 5, 52 -- Received: from } NAM03-DM3-obe.outbound.protection.outlook.com } (mail-dm3nam03on0101.outbound.protection.outlook.com [104.47.41.101]) } 5, 52 -- by microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id v3JBtx6R013907 5, 52 -- for } {microscopy-at-microscopy.com} ; Wed, 19 Apr 2017 06:56:00 -0500 5, 52 -- } DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 5, 52 -- } d=CentralMichigan.onmicrosoft.com; s=selector1-cmich-edu; 5, 52 -- } h=From:Date:Subject:Message-ID:Content-Type:MIME-Version; 5, 52 -- } bh=hcWmMrleacIGpyJQ25MfEWO1TNCzVbdd+Pms167S/fo=; 5, 52 -- } b=jyVpLMrxjUjopCT91rLar2F8CZDjvnzKPX4nzyP4uwDOaMrnnrnDVYV7m05pcxC6GP0BpDfmwFUgWgiQOs7LMXCICvVH5rd05MNai6RVzpiqWdb0inAFKfjf97aSjLKyaaS2SpTe5Yz2YVerBK1zzJF0yLSsgPuWr2ElssP+G58= } 5, 52 -- Received: from DM2PR05MB768.namprd05.prod.outlook.com } (10.141.179.27) by 5, 52 -- DM2PR05MB766.namprd05.prod.outlook.com } (10.141.179.21) with Microsoft SMTP 5, 52 -- Server (version=TLS1_2, } cipher=TLS_ECDHE_RSA_WITH_AES_128_CBC_SHA256_P256) id 5, 52 -- } 15.1.1034.5; Wed, 19 Apr 2017 12:06:14 +0000 5, 52 -- Received: from } DM2PR05MB768.namprd05.prod.outlook.com ([10.141.179.27]) by 5, 52 -- } DM2PR05MB768.namprd05.prod.outlook.com ([10.141.179.27]) with mapi id } 5, 52 -- 15.01.1034.018; Wed, 19 Apr 2017 12:06:13 +0000 5, 52 -- } From: "Oshel, Philip Eugene" {oshel1pe-at-cmich.edu} 5, 52 -- To: } "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 5, 52 -- } Subject: FW: Ask a Microscopist- converting EDS files in .spx format } 5, 52 -- Thread-Topic: Ask a Microscopist- converting EDS files } in .spx format 5, 52 -- Thread-Index: } AQHSuQVXzz7A+NwUlkmEfW6nF4OSKA== 5, 52 -- Date: Wed, 19 Apr 2017 } 12:06:13 +0000 5, 52 -- Message-ID: } {8AC6DB5C-2711-4C23-AEEA-003385C90AC3-at-cmich.edu} 5, 52 -- } Accept-Language: en-US 5, 52 -- Content-Language: en-US 5, 52 -- } X-MS-Has-Attach: 5, 52 -- X-MS-TNEF-Correlator: 5, 52 -- } authentication-results: microscopy.com; dkim=none (message not } signed) 5, 52 -- header.d=none;microscopy.com; dmarc=none } action=none header.from=cmich.edu; 5, 52 -- x-originating-ip: } [141.209.133.243] 5, 52 -- x-ms-publictraffictype: Email 5, 52 -- } x-microsoft-exchange-diagnostics: } 1;DM2PR05MB766;7:aKtia4607Ia0/apKvHbE5M7tSh3pbTA4woj2TDyLpEwnfL8CExwXmAgTFAb/2NkLmtz5MhlE+85x03Mm9WUfC8tT/po6mxLZPig4qmN/z+yur4mGsAUmb5rImEUD8EtiVg6i23QNUbyvPFgeYS/IKke8Hf7MMBnA16z7P7Wq02C7k2K29alNzCgDl9GP5KOpHy2Fe/j0UD4SSOMr0YNrj77nZoHXLiWTefUzfTNzCRvLcccSYJor1sO83wjkbrK16MCd+PMaN9XbdvsmIyyj7JZbCbnicXGt2wpKsM9wCbznCxdCWr+dRVwVi/lwqAGrHCGs0d5W8uC8eH/BvvrRUg== } 5, 52 -- x-ms-office365-filtering-correlation-id: } 1ba13578-c167-4b25-cd66-08d4871c79df 5, 52 -- x-microsoft-antispam: } UriScan:;BCL:0;PCL:0;RULEID:(22001)(2017030254075)(201703131423075)(201703031133081);SRVR:DM2PR05MB766; } 5, 52 -- x-microsoft-antispam-prvs: } {DM2PR05MB766E273163D50CB13FB006895180-at-DM2PR05MB766.namprd05.prod.outlook.com} } 5, 52 -- x-exchange-antispam-report-test: UriScan:; 5, 52 -- } x-exchange-antispam-report-cfa-test: } BCL:0;PCL:0;RULEID:(11241501159)(6040450)(601004)(2401047)(8121501046)(5005006)(10201501046)(93006095)(93001095)(3002001)(6041248)(201703131423075)(201702281529075)(201702281528075)(201703061421075)(20161123564025)(20161123560025)(20161123555025)(20161123562025)(6072148);SRVR:DM2PR05MB766;BCL:0;PCL:0;RULEID:;SRVR:DM2PR05MB766; } 5, 52 -- x-forefront-prvs: 028256169F 5, 52 -- } x-forefront-antispam-report: } SFV:NSPM;SFS:(10019020)(6009001)(39450400003)(39840400002)(39850400002)(39410400002)(39400400002)(13464003)(33656002)(305945005)(7736002)(54356999)(50986999)(66066001)(86362001)(2501003)(2900100001)(38730400002)(6116002)(122556002)(2351001)(102836003)(3846002)(110136004)(53546009)(189998001)(25786009)(1730700003)(81166006)(53936002)(229853002)(2473003)(6512007)(99286003)(5660300001)(6916009)(8676002)(8936002)(3660700001)(83716003)(82746002)(3280700002)(36756003)(6506006)(5640700003)(6436002)(88552002)(77096006)(6486002)(2906002)(142923001);DIR:OUT;SFP:1102;SCL:1;SRVR:DM2PR05MB766;H:DM2PR05MB768.namprd05.prod.outlook.com;FPR:;SPF:None;MLV:sfv;LANG:en; } 5, 52 -- spamdiagnosticoutput: 1:99 5, 52 -- spamdiagnosticmetadata: } NSPM 5, 52 -- Content-Type: text/plain; charset="utf-8" 5, 52 -- } Content-ID: } {F1C8A67B7EC0064D8ECA4B92CA8A49BF-at-namprd05.prod.outlook.com} 5, 52 -- } MIME-Version: 1.0 5, 52 -- X-OriginatorOrg: cmich.edu 5, 52 -- } X-MS-Exchange-CrossTenant-originalarrivaltime: 19 Apr 2017 } 12:06:13.7104 5, 52 -- (UTC) 5, 52 -- } X-MS-Exchange-CrossTenant-fromentityheader: Hosted 5, 52 -- } X-MS-Exchange-CrossTenant-id: c871bc6e-7cc6-4a57-a4eb-22309fc34963 5, } 52 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: DM2PR05MB766 } 5, 52 -- Content-Transfer-Encoding: 8bit 5, 52 -- } X-MIME-Autoconverted: from base64 to 8bit by microscopy.com id } v3JBtx6R013907 ==============================End of - } Headers==============================
==============================Original Headers============================== 8, 50 -- From benada-at-biomed.cas.cz Wed Apr 19 09:52:11 2017 8, 50 -- Received: from barracuda.biomed.cas.cz (barracuda.biomed.cas.cz [147.231.40.11]) 8, 50 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v3JEqAHD007526 8, 50 -- for {microscopy-at-microscopy.com} ; Wed, 19 Apr 2017 09:52:11 -0500 8, 50 -- X-ASG-Debug-ID: 1492614144-05011e10cb1abd10001-4CH8be 8, 50 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) by barracuda.biomed.cas.cz with ESMTP id sAGGIttF1QvZSiIg; Wed, 19 Apr 2017 17:02:24 +0200 (CEST) 8, 50 -- X-Barracuda-Envelope-From: benada-at-biomed.cas.cz 8, 50 -- X-Barracuda-Effective-Source-IP: mail2.biomed.cas.cz[147.231.40.32] 8, 50 -- X-Barracuda-Apparent-Source-IP: 147.231.40.32 8, 50 -- Received: from u117ob02 (nb170ph.mbu.cas.cz [147.231.44.133]) 8, 50 -- (using TLSv1.2 with cipher ECDHE-RSA-AES128-GCM-SHA256 (128/128 bits)) 8, 50 -- (No client certificate requested) 8, 50 -- by mail2.biomed.cas.cz (Postfix) with ESMTPSA id 57C90D015B6; 8, 50 -- Wed, 19 Apr 2017 17:02:23 +0200 (CEST) 8, 50 -- Date: Wed, 19 Apr 2017 17:02:22 +0200 8, 50 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 8, 50 -- To: anuja.bhalkikar-at-huskers.unl.edu, {microscopy-at-microscopy.com} 8, 50 -- Subject: Re: [Microscopy] FW: Ask a Microscopist- converting EDS files in 8, 50 -- .spx format 8, 50 -- Message-ID: {20170419170222.6b20441e-at-u117ob02} 8, 50 -- X-ASG-Orig-Subj: Re: [Microscopy] FW: Ask a Microscopist- converting EDS files in 8, 50 -- .spx format 8, 50 -- In-Reply-To: {201704191159.v3JBxB2v016688-at-microscopy.com} 8, 50 -- References: {201704191159.v3JBxB2v016688-at-microscopy.com} 8, 50 -- Organization: =?UTF-8?B?TWlrcm9iaW9sb2dpY2vDvSDDunN0YXY=?= AV 8, 50 -- =?UTF-8?B?xIxS?= 8, 50 -- X-Mailer: Claws Mail 3.14.1 (GTK+ 2.24.25; i586-pc-linux-gnu) 8, 50 -- MIME-Version: 1.0 8, 50 -- Content-Type: text/plain; charset=UTF-8 8, 50 -- X-IoP-CAS-MailScanner-Information: Please contact the ISP for more information 8, 50 -- X-IoP-CAS-MailScanner-ID: 57C90D015B6.A97F8 8, 50 -- X-IoP-CAS-MailScanner: Processed 8, 50 -- X-Spam-Status: No 8, 50 -- X-Barracuda-Connect: mail2.biomed.cas.cz[147.231.40.32] 8, 50 -- X-Barracuda-Start-Time: 1492614144 8, 50 -- X-Barracuda-URL: https://barracuda.biomed.cas.cz:443/cgi-mod/mark.cgi 8, 50 -- X-Barracuda-Scan-Msg-Size: 7539 8, 50 -- X-Virus-Scanned: by bsmtpd at biomed.cas.cz 8, 50 -- X-Barracuda-BRTS-Status: 1 8, 50 -- X-Barracuda-Spam-Score: 1.10 8, 50 -- X-Barracuda-Spam-Status: No, SCORE=1.10 using per-user scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=3.0 tests=BSF_RULE7568M, BSF_RULE_7582B, FB_LETTERS_21B 8, 50 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.38191 8, 50 -- Rule breakdown below 8, 50 -- pts rule name description 8, 50 -- ---- ---------------------- -------------------------------------------------- 8, 50 -- 0.10 FB_LETTERS_21B BODY: Special people leave special signs! 8, 50 -- 0.50 BSF_RULE_7582B Custom Rule 7582B 8, 50 -- 0.50 BSF_RULE7568M Custom Rule 7568M 8, 50 -- Content-Transfer-Encoding: 8bit 8, 50 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v3JEqAHD007526 ==============================End of - Headers==============================
I’m trying to round out my understanding of different embedding media and need the latest opinions on Spurr’s LVEM.
I have used it in the past, it was OK, and never worried about it.
Now I see that some workers avoid it due to more toxic ingredients, more difficulty staining, and problems with brittle blocks. Also, like Epon 812 before, I see that some of the original components have been replaced with newer substitutes.
I teach students how to embed tissue and want to be able to tell them the latest opinions from other experts in case I am missing something.
Thanks
Jon
Jonathan Krupp ASB&T San Joaquin Delta College 5151Pacific Ave. Stockton, CA 95207 (209) 954-5284 jkrupp-at-deltacollege.edu
==============================Original Headers============================== 10, 40 -- From prvs=1275be5884=jkrupp-at-deltacollege.edu Wed Apr 19 15:29:05 2017 10, 40 -- Received: from mailin2.deltacollege.edu (mailin2.deltacollege.edu [207.62.175.116]) 10, 40 -- by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v3JKT46R005744 10, 40 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Apr 2017 15:29:05 -0500 10, 40 -- Received: from mailin2.deltacollege.edu (localhost.localdomain [127.0.0.1]) 10, 40 -- by localhost (Email Security Appliance) with SMTP id 89BE8175650_8F7CAF9B 10, 40 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Apr 2017 20:39:21 +0000 (GMT) 10, 40 -- Received: from zmail.deltacollege.edu (zmail.deltacampus.net [10.200.68.180]) 10, 40 -- (using TLSv1.2 with cipher ECDHE-RSA-AES256-GCM-SHA384 (256/256 bits)) 10, 40 -- (Client did not present a certificate) 10, 40 -- by mailin2.deltacollege.edu (Sophos Email Appliance) with ESMTPS id 6C90D173A9C_8F7CAF9F 10, 40 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Apr 2017 20:39:21 +0000 (GMT) 10, 40 -- Received: from localhost (localhost [127.0.0.1]) 10, 40 -- by zmail.deltacollege.edu (Postfix) with ESMTP id CF2666D1E741 10, 40 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Apr 2017 13:30:39 -0700 (PDT) 10, 40 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 10, 40 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10032) 10, 40 -- with ESMTP id XOMOuOHh5UxU for {Microscopy-at-microscopy.com} ; 10, 40 -- Wed, 19 Apr 2017 13:30:39 -0700 (PDT) 10, 40 -- Received: from localhost (localhost [127.0.0.1]) 10, 40 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 62C1C6D1E743 10, 40 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Apr 2017 13:30:39 -0700 (PDT) 10, 40 -- X-Virus-Scanned: amavisd-new at zmail.deltacollege.edu 10, 40 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 10, 40 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10026) 10, 40 -- with ESMTP id 10_cxiZXKJ3k for {Microscopy-at-microscopy.com} ; 10, 40 -- Wed, 19 Apr 2017 13:30:39 -0700 (PDT) 10, 40 -- Received: from [10.246.128.227] (unknown [10.246.128.227]) 10, 40 -- by zmail.deltacollege.edu (Postfix) with ESMTPSA id 41F186D1E741 10, 40 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Apr 2017 13:30:39 -0700 (PDT) 10, 40 -- From: Jonathan Krupp {jkrupp-at-deltacollege.edu} 10, 40 -- Content-Type: text/plain; charset=utf-8 10, 40 -- Subject: Update on Spurr's Plastic Please 10, 40 -- Message-Id: {0F894793-1B18-4B1B-9E76-75ED78A9F395-at-deltacollege.edu} 10, 40 -- Date: Wed, 19 Apr 2017 13:39:20 -0700 10, 40 -- To: Microscopy-at-microscopy.com 10, 40 -- Mime-Version: 1.0 (Mac OS X Mail 9.3 \(3124\)) 10, 40 -- X-Mailer: Apple Mail (2.3124) 10, 40 -- Content-Transfer-Encoding: 8bit 10, 40 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v3JKT46R005744 ==============================End of - Headers==============================
Check out the Oxford text: high Resolution Electron Microscopy by Spence.
A close second and less Graduate level math is found in "Transmission Electron Microscopy and Diffractory Materials" Springer by Fultz and Howe.
You are looking for a discussion of "Pendellosung". Page 631 Fultz and Howe. To explain it to Students at U of O that I taught, I built a coupled pendulum, where the one pendulum represents the non diffracted beam and the other s Bragg reflected beam. The coupling represents the lattice. The pendulum will stop and start and that time can represent thickness of da sample. And so white spots in HRTEM , nodes, could be channels between atoms or atoms depending on how thick the sample is ( how much time pendulum swings )
Pete Eschbach Oregon State University
Sent from my iPhone
} On Apr 18, 2017, at 4:47 PM, kleopullin-at-email.arizona.edu wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I'm struggling with Williams and Carter (2nd edition, I think) } understanding HRTEM. I generally find their text approachable and easy } to read, but not the HRTEM material, other than the math. } } Is their a text or article that is more detailed? Outside of the } Supposedly HRTEM sources that I have found have lengthy introductions } to the basic, non-HR microscope, then brief descriptions of the math } of HRTEM. } } What's a good Read? } } I'm a microscopist, with a B.S., so technical is okay, but I want a } deep focus on HRTEM, theory and instrumentation. } } Thanks! } } Kleo (Kathleen) Pullin } Moraga, CA } 209-610-0555 } kleopullin-at-email.arizona.edu } https://www.linkedin.com/in/kleopullin } https://twitter.com/resolvingdust } } ==============================Original Headers============================== } 6, 38 -- From kleopullin-at-email.arizona.edu Tue Apr 18 18:31:43 2017 } 6, 38 -- Received: from mail-lf0-f45.google.com (mail-lf0-f45.google.com [209.85.215.45]) } 6, 38 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v3INVhXS006989 } 6, 38 -- for {Microscopy-at-microscopy.com} ; Tue, 18 Apr 2017 18:31:43 -0500 } 6, 38 -- Received: by mail-lf0-f45.google.com with SMTP id c80so3945116lfh.3 } 6, 38 -- for {Microscopy-at-microscopy.com} ; Tue, 18 Apr 2017 16:41:57 -0700 (PDT) } 6, 38 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 6, 38 -- d=email-arizona-edu.20150623.gappssmtp.com; s=20150623; } 6, 38 -- h=mime-version:from:date:message-id:subject:to; } 6, 38 -- bh=YfdPdRMwFgmXu5BxNvOKsAkoyo+FwZmkn/NoYBoF2y4=; } 6, 38 -- b=BoDK4h27IShxyrd1enChdx9i3aRM10mCT1+5WFi/eEOnTpWZyIGh0pE1g84f1prfv6 } 6, 38 -- dd7Rgexw/nxcbCGZHzVeM9m7zS3TG08A25Zcyrr4SG38tzHvMJn2IcEN/yK2A9KGMo4P } 6, 38 -- ppt4fo5K4EujC2IjSYzDaAwgDo993PSlFfmK354jP8+6biDy5P5Re769GHGlXNxaRFoO } 6, 38 -- vIffa07ko2UYxW2ZtSk32ndCYjGInF7YWeJj0fMr/Q2QH9d8xqGKYlecyBZ9icvq8Gf3 } 6, 38 -- COcKwvK2ekJjd5OclkFdZV12L1iS2TvnPKVY1jdmaSw2KcIeP4hRHaapfyPeZbudzl/9 } 6, 38 -- zQ8A== } 6, 38 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 6, 38 -- d=1e100.net; s=20161025; } 6, 38 -- h=x-gm-message-state:mime-version:from:date:message-id:subject:to; } 6, 38 -- bh=YfdPdRMwFgmXu5BxNvOKsAkoyo+FwZmkn/NoYBoF2y4=; } 6, 38 -- b=gVQL5HThM8f9zp8rMP2VBkjlLysPC5OKKkRq6+r111mxDyJYKFz6j/50hS0G5iuJS8 } 6, 38 -- MESmhhiLeJ+oJWp+J4KzPLvKtJVKZF+UCBPtso7MarAMj0n38n7GA47PVWdntUqiBEnq } 6, 38 -- 4mxXvPxZLYeVkVQ5ovp2l06e7yv1YAkWKT83CZCGmnuOblirQ2NMAoueFWZlB0cPOaxO } 6, 38 -- axGH1jvd2Bvk9Vf/wNhjkEB2Emtm6bH/9THQAheemIXeUpN1xwUi3jDDwcPdZIZqcNDK } 6, 38 -- +by3rgzzHYHLcMa6iaf5kdbcaaTwJcN2NEGXxh9qxW8ajqyyaWNdErCRGL2fn6I8RlRh } 6, 38 -- gM7w== } 6, 38 -- X-Gm-Message-State: AN3rC/4yqfN7txLl177I6Cvh7OECVqoJ5gau5WT9Q4TZXj97g1Ql8ycD } 6, 38 -- qTWclIj/HA1/CdwHeA1RMfP9aM0Ktf8M } 6, 38 -- X-Received: by 10.46.6.17 with SMTP id 17mr2449244ljg.125.1492558916687; Tue, } 6, 38 -- 18 Apr 2017 16:41:56 -0700 (PDT) } 6, 38 -- MIME-Version: 1.0 } 6, 38 -- Received: by 10.25.166.145 with HTTP; Tue, 18 Apr 2017 16:41:56 -0700 (PDT) } 6, 38 -- From: Kathleen Pullin {kleopullin-at-email.arizona.edu} } 6, 38 -- Date: Tue, 18 Apr 2017 16:41:56 -0700 } 6, 38 -- Message-ID: {CAKUce=RSMa2JP0Q3M_9d8zTaBvp_GOx3zQEYmN9FVnRtxLuVXA-at-mail.gmail.com} } 6, 38 -- Subject: HRTEM } 6, 38 -- To: Microscopy-at-microscopy.com } 6, 38 -- Content-Type: text/plain; charset=UTF-8 } ==============================End of - Headers==============================
After trying Epon, I’ve never felt the need to use Spurr's again. For some reason the Spurr’s recipe proportions vary between some vendors. I’ve been really happy with the LX-112 (Epon) from LaddResearch, their mixing instruction are right on point, and I can store the 2 components in syringes in the fridge for about 6 months and just mix them with the accelerator prior to use. Epon seem to section better than Spurr's, and is more stable under the beam. I've also heard good things about the Epon-Araldite mixture.
-Joe
Joe Mowery | Biologist Electron and Confocal Microscopy Unit USDA, Agricultural Research Service Beltsville, MD
-----Original Message----- X-from: jkrupp-at-deltacollege.edu [mailto:jkrupp-at-deltacollege.edu] Sent: Wednesday, April 19, 2017 4:49 PM To: Mowery, Joseph
Greetings
I’m trying to round out my understanding of different embedding media and need the latest opinions on Spurr’s LVEM.
I have used it in the past, it was OK, and never worried about it.
Now I see that some workers avoid it due to more toxic ingredients, more difficulty staining, and problems with brittle blocks. Also, like Epon 812 before, I see that some of the original components have been replaced with newer substitutes.
I teach students how to embed tissue and want to be able to tell them the latest opinions from other experts in case I am missing something.
Thanks
Jon
Jonathan Krupp ASB&T San Joaquin Delta College 5151Pacific Ave. Stockton, CA 95207 (209) 954-5284 jkrupp-at-deltacollege.edu
==============================Original Headers============================== 10, 40 -- From prvs=1275be5884=jkrupp-at-deltacollege.edu Wed Apr 19 15:29:05 2017 10, 40 -- Received: from mailin2.deltacollege.edu (mailin2.deltacollege.edu [207.62.175.116]) 10, 40 -- by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v3JKT46R005744 10, 40 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Apr 2017 15:29:05 -0500 10, 40 -- Received: from mailin2.deltacollege.edu (localhost.localdomain [127.0.0.1]) 10, 40 -- by localhost (Email Security Appliance) with SMTP id 89BE8175650_8F7CAF9B 10, 40 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Apr 2017 20:39:21 +0000 (GMT) 10, 40 -- Received: from zmail.deltacollege.edu (zmail.deltacampus.net [10.200.68.180]) 10, 40 -- (using TLSv1.2 with cipher ECDHE-RSA-AES256-GCM-SHA384 (256/256 bits)) 10, 40 -- (Client did not present a certificate) 10, 40 -- by mailin2.deltacollege.edu (Sophos Email Appliance) with ESMTPS id 6C90D173A9C_8F7CAF9F 10, 40 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Apr 2017 20:39:21 +0000 (GMT) 10, 40 -- Received: from localhost (localhost [127.0.0.1]) 10, 40 -- by zmail.deltacollege.edu (Postfix) with ESMTP id CF2666D1E741 10, 40 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Apr 2017 13:30:39 -0700 (PDT) 10, 40 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 10, 40 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10032) 10, 40 -- with ESMTP id XOMOuOHh5UxU for {Microscopy-at-microscopy.com} ; 10, 40 -- Wed, 19 Apr 2017 13:30:39 -0700 (PDT) 10, 40 -- Received: from localhost (localhost [127.0.0.1]) 10, 40 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 62C1C6D1E743 10, 40 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Apr 2017 13:30:39 -0700 (PDT) 10, 40 -- X-Virus-Scanned: amavisd-new at zmail.deltacollege.edu 10, 40 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 10, 40 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10026) 10, 40 -- with ESMTP id 10_cxiZXKJ3k for {Microscopy-at-microscopy.com} ; 10, 40 -- Wed, 19 Apr 2017 13:30:39 -0700 (PDT) 10, 40 -- Received: from [10.246.128.227] (unknown [10.246.128.227]) 10, 40 -- by zmail.deltacollege.edu (Postfix) with ESMTPSA id 41F186D1E741 10, 40 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Apr 2017 13:30:39 -0700 (PDT) 10, 40 -- From: Jonathan Krupp {jkrupp-at-deltacollege.edu} 10, 40 -- Content-Type: text/plain; charset=utf-8 10, 40 -- Subject: Update on Spurr's Plastic Please 10, 40 -- Message-Id: {0F894793-1B18-4B1B-9E76-75ED78A9F395-at-deltacollege.edu} 10, 40 -- Date: Wed, 19 Apr 2017 13:39:20 -0700 10, 40 -- To: Microscopy-at-microscopy.com 10, 40 -- Mime-Version: 1.0 (Mac OS X Mail 9.3 \(3124\)) 10, 40 -- X-Mailer: Apple Mail (2.3124) 10, 40 -- Content-Transfer-Encoding: 8bit 10, 40 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v3JKT46R005744 ==============================End of - Headers==============================
This electronic message contains information generated by the USDA solely for the intended recipients. Any unauthorized interception of this message or the use or disclosure of the information it contains may violate the law and subject the violator to civil or criminal penalties. If you believe you have received this message in error, please notify the sender and delete the email immediately.
Dear All, We are interested in anyone having an old Gatan GIF 200, or a more recent EEL spectrometer for a Tecnai 30, who needs to have it taken off their hands. Thanks,
Ken
Johns Hopkins University
==============================Original Headers============================== 3, 34 -- From prvs=276f74c40=klivi-at-jhu.edu Thu Apr 20 13:38:21 2017 3, 34 -- Received: from IronEB5.johnshopkins.edu (ironeb5.johnshopkins.edu [162.129.199.150]) 3, 34 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v3KIcLkC006899 3, 34 -- for {microscopy-at-microscopy.com} ; Thu, 20 Apr 2017 13:38:21 -0500 3, 34 -- X-IronPort-AV: E=Sophos;i="5.37,225,1488862800"; 3, 34 -- d="scan'208";a="91725755" 3, 34 -- Received: from esgebex1.win.ad.jhu.edu ([10.15.89.60]) 3, 34 -- by IronEB5.johnshopkins.edu with ESMTP/TLS/AES256-SHA; 20 Apr 2017 14:48:41 -0400 3, 34 -- Received: from ESGMTWEX8.win.ad.jhu.edu (10.181.25.143) by 3, 34 -- ESGEBEX1.win.ad.jhu.edu (10.15.89.60) with Microsoft SMTP Server (TLS) id 3, 34 -- 15.0.1210.3; Thu, 20 Apr 2017 14:48:41 -0400 3, 34 -- Received: from ESGMTWEX8.win.ad.jhu.edu ([fe80::8c05:28af:ae73:d0eb]) by 3, 34 -- ESGMTWEX8.win.ad.jhu.edu ([fe80::8c05:28af:ae73:d0eb%24]) with mapi id 3, 34 -- 15.00.1210.000; Thu, 20 Apr 2017 14:48:41 -0400 3, 34 -- From: KEN Livi {klivi-at-jhu.edu} 3, 34 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 3, 34 -- CC: KEN Livi {klivi-at-jhu.edu} 3, 34 -- Subject: EELS 3, 34 -- Thread-Topic: EELS 3, 34 -- Thread-Index: AQHSuga6/Zn9V/eXtke2jVnlXsUD/Q== 3, 34 -- Date: Thu, 20 Apr 2017 18:48:40 +0000 3, 34 -- Message-ID: {EF2A1D19-DA44-49E2-AA1F-D8F9631910A3-at-jhu.edu} 3, 34 -- Accept-Language: en-US 3, 34 -- Content-Language: en-US 3, 34 -- X-MS-Has-Attach: 3, 34 -- X-MS-TNEF-Correlator: 3, 34 -- x-ms-exchange-messagesentrepresentingtype: 1 3, 34 -- x-ms-exchange-transport-fromentityheader: Hosted 3, 34 -- x-originating-ip: [10.181.198.109] 3, 34 -- Content-Type: text/plain; charset="us-ascii" 3, 34 -- Content-ID: {7B5D5C3250E66D4883B2F6D70A84D5CF-at-exchange.johnshopkins.edu} 3, 34 -- MIME-Version: 1.0 3, 34 -- Content-Transfer-Encoding: 8bit 3, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v3KIcLkC006899 ==============================End of - Headers==============================
From watkinsmelanie58-at-gmail.com Mon Apr 24 19:52:57 2017 Return-Path: {watkinsmelanie58-at-gmail.com} Received: from gmail.com ([218.232.94.109]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v3P0qs2Q014216 for {microscopylistserverarchive6-at-microscopy.com} ; Mon, 24 Apr 2017 19:52:56 -0500 Message-ID: {7000395D.B9311C48-at-gmail.com}
Here at UC Riverside we have a Philips XL30-FEG SEM that we are planning to remove from service. If anyone is interested in acquiring this system please contact us.
The SEM is in perfect fully operational condition. It was installed in 1996 and it has been under continuous parts and labor maintenance contract with the FEI Co. since. Here is the configuration of the system:
FEG Schottky field emitter Continuously adjustable accelerating voltage from 200 V to 30 kV Resolution 2.0 nm at 30 kV 5.0 nm at 1 kV Detectors Everhard-Thornley SE/BSE (ETD) Solid-state BSED Manually-controilled specimen stage. IGP and diffusion pump vacuum system specimen chamber with 52-pin electrical feedthrough for electrical measurements the original Windows 3.11 PC has been ungraded to Windows 2000 PC. Digital image acquisition as well as Polaroid camera.
Analytical System EDAX Inc. Phoenix/Genesis EDX system liquid N2 cooled Si(Li) EDX detector 10mm2 Resolution Mn Kα - 129 eV SATW window for detection of elements from Beryllium up EBSD system - Hamamatsu video camera and HKL Channel5 software package for EBSD data acquisition and analysis
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both stacie-at-ems-secure.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Aurion and Bio SEM Workshops
Message: Electron Microscopy Sciences welcomes Peter van de Plas of Aurion to its Microscopy Academy May 10-12 for Immuno Gold Silver Staining Workshop - contact me for more information or register here: http://www.emsdiasum.com/microscopy/academy/courses/immunogold.aspx
Also June 6-8, we are holding a Biological SEM Workshop! This course will introduce participants to methods of sample preparation and SEM parameters and operation needed for accurate analysis. You can find out more about it here:
EMS Microscopy Academy hopes to provide a cost-effective means of personalized instruction so you can learn what you need to get the job done in as short a time possible. It's our way of giving back to a community that has supported us for over 40 years.
Login Host: 73.165.127.32 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
Technically you can heat the solution to try and depolymerize the presumptive paraformaldehyde oligomers. I do *not* recommend trying this - it works occasionally, but hot formalin is almost never a good idea even in a hood.
Option 1: Send it to Haz Waste and buy another bottle. Option 2: Filter it and use it anyway.
I've needed to use option 2 a few times over the years - mostly due to reagent shipping issues and/or "surprise!" samples. In my hands, filtered PF works okay for straight histology-level work, so-so (at best) for immuno work, and leads to a range of....interesting results for EM samples.
I'd still recommend option 1 - formalin is { $10/liter from Fisher. In a pinch, filtering for H&E or other optical microscopy will probably be okay.
Aaron
----- Aaron Barnes, MD, PhD Clinical Pathology Resident (PGY1) | Dept of Lab Medicine & Pathology University of Minnesota Medical School Dunny Lab | Dept of Microbiology & Immunology
On Wed, Apr 26, 2017 at 3:33 PM, {jkrupp-at-deltacollege.edu} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I have a bottle of Formalin that has a white precipitate, probably polymerized formaldehyde from being stored in the refrigerator. } } Is there anything I can do to return the solution to normal? } } Thanks } } Jon } } Jonathan Krupp } ASB&T } San Joaquin Delta College } 5151Pacific Ave. } Stockton, CA 95207 } (209) 954-5284 } jkrupp-at-deltacollege.edu } } } } ==============================Original Headers============================== } 7, 40 -- From prvs=1282bc8008=jkrupp-at-deltacollege.edu Wed Apr 26 15:23:13 2017 } 7, 40 -- Received: from mailin2.deltacollege.edu (mailin2.deltacollege.edu [207.62.175.116]) } 7, 40 -- by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v3QKNCj6024425 } 7, 40 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Apr 2017 15:23:13 -0500 } 7, 40 -- Received: from mailin2.deltacollege.edu (localhost.localdomain [127.0.0.1]) } 7, 40 -- by localhost (Email Security Appliance) with SMTP id 11C471756CB_9010433B } 7, 40 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Apr 2017 20:33:55 +0000 (GMT) } 7, 40 -- Received: from zmail.deltacollege.edu (zmail.deltacampus.net [10.200.68.180]) } 7, 40 -- (using TLSv1.2 with cipher ECDHE-RSA-AES256-GCM-SHA384 (256/256 bits)) } 7, 40 -- (Client did not present a certificate) } 7, 40 -- by mailin2.deltacollege.edu (Sophos Email Appliance) with ESMTPS id EA0591731B3_9010432F } 7, 40 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Apr 2017 20:33:54 +0000 (GMT) } 7, 40 -- Received: from localhost (localhost [127.0.0.1]) } 7, 40 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 5CECD6D1E742 } 7, 40 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Apr 2017 13:24:40 -0700 (PDT) } 7, 40 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) } 7, 40 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10032) } 7, 40 -- with ESMTP id l5ZvxTGGZea6 for {Microscopy-at-microscopy.com} ; } 7, 40 -- Wed, 26 Apr 2017 13:24:40 -0700 (PDT) } 7, 40 -- Received: from localhost (localhost [127.0.0.1]) } 7, 40 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 072236D1E743 } 7, 40 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Apr 2017 13:24:40 -0700 (PDT) } 7, 40 -- X-Virus-Scanned: amavisd-new at zmail.deltacollege.edu } 7, 40 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) } 7, 40 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10026) } 7, 40 -- with ESMTP id uVlGwa77gaUj for {Microscopy-at-microscopy.com} ; } 7, 40 -- Wed, 26 Apr 2017 13:24:39 -0700 (PDT) } 7, 40 -- Received: from [10.246.133.69] (unknown [10.246.133.69]) } 7, 40 -- by zmail.deltacollege.edu (Postfix) with ESMTPSA id D4FDB6D1E741 } 7, 40 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Apr 2017 13:24:39 -0700 (PDT) } 7, 40 -- From: Jonathan Krupp {jkrupp-at-deltacollege.edu} } 7, 40 -- Content-Type: text/plain; charset=us-ascii } 7, 40 -- Subject: Formalin } 7, 40 -- Message-Id: {3DF87366-FB86-4401-87C2-D9A31DA433AB-at-deltacollege.edu} } 7, 40 -- Date: Wed, 26 Apr 2017 13:33:54 -0700 } 7, 40 -- To: Microscopy-at-microscopy.com } 7, 40 -- Mime-Version: 1.0 (Mac OS X Mail 9.3 \(3124\)) } 7, 40 -- X-Mailer: Apple Mail (2.3124) } 7, 40 -- Content-Transfer-Encoding: 8bit } 7, 40 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v3QKNCj6024425 } ==============================End of - Headers==============================
==============================Original Headers============================== 9, 61 -- From barnesa-at-umn.edu Wed Apr 26 15:49:32 2017 9, 61 -- Received: from mta-p5.oit.umn.edu (mta-p5.oit.umn.edu [134.84.196.205]) 9, 61 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v3QKnW0D015195 9, 61 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Apr 2017 15:49:32 -0500 9, 61 -- Received: from localhost (unknown [127.0.0.1]) 9, 61 -- by mta-p5.oit.umn.edu (Postfix) with ESMTP id 1DF9541C 9, 61 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Apr 2017 21:00:15 +0000 (UTC) 9, 61 -- X-Virus-Scanned: amavisd-new at umn.edu 9, 61 -- Received: from mta-p5.oit.umn.edu ([127.0.0.1]) 9, 61 -- by localhost (mta-p5.oit.umn.edu [127.0.0.1]) (amavisd-new, port 10024) 9, 61 -- with ESMTP id 4d-dCRblKeaI for {Microscopy-at-microscopy.com} ; 9, 61 -- Wed, 26 Apr 2017 16:00:14 -0500 (CDT) 9, 61 -- Received: from dlp-agent-p5.oit.umn.edu (dlp-agent-p5.oit.umn.edu [134.84.189.205]) 9, 61 -- (using TLSv1.2 with cipher AES256-SHA (256/256 bits)) 9, 61 -- (No client certificate requested) 9, 61 -- by mta-p5.oit.umn.edu (Postfix) with ESMTPS id D9412A96 9, 61 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Apr 2017 16:00:14 -0500 (CDT) 9, 61 -- Received: from pps.filterd (dlp-agent-p5.oit.umn.edu [127.0.0.1]) 9, 61 -- by dlp-agent-p5.oit.umn.edu (8.16.0.17/8.16.0.17) with SMTP id v3QBJZbP022032 9, 61 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Apr 2017 16:00:14 -0500 9, 61 -- Received: from mail-lf0-f71.google.com (mail-lf0-f71.google.com [209.85.215.71]) 9, 61 -- by dlp-agent-p5.oit.umn.edu with ESMTP id 2a019a1kr1-1 9, 61 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128 verify=NOT) 9, 61 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Apr 2017 16:00:14 -0500 9, 61 -- Received: by mail-lf0-f71.google.com with SMTP id d127so2053967lfe.10 9, 61 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Apr 2017 14:00:14 -0700 (PDT) 9, 61 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 9, 61 -- d=umn.edu; s=google; 9, 61 -- h=mime-version:from:date:message-id:subject:to; 9, 61 -- bh=JUc3ShqyOyLhcuhl/KCPREK97kH+LF+g61c5XJNBqk4=; 9, 61 -- b=mDJRRNzFqJA2f56yNLvbLsWDtQ6H64MTQNK6jPrw9bMINZj9gSIdQ+fBYOK6kRy4Mq 9, 61 -- EyB/A0LYrMLm6bBzQu1BGPfy/tJDSvVL6NPjBtZTaVNYFRpu8/NCtGQgFV0rdwCBjXG2 9, 61 -- bHEf1oYT42OcxoiBGI6J7bOwn2+dFa7MnCj8ol8eyjvZDi4JSmNd8e1UBiAH0aXAmKH+ 9, 61 -- gOP2ieDB1mPt5epEwxaSWDfwtBiUJ0IynIvfBDzUjqTWQ4gqKCZLUvZ9hM+z+dQCtZhL 9, 61 -- ngwFR9XJRzrZBG1xLJ5mUokar67qLtsgl67RwR/k6+GvME+gdbQHbngTqjiI31ePUEW7 9, 61 -- bm+g== 9, 61 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 9, 61 -- d=1e100.net; s=20161025; 9, 61 -- h=x-gm-message-state:mime-version:from:date:message-id:subject:to; 9, 61 -- bh=JUc3ShqyOyLhcuhl/KCPREK97kH+LF+g61c5XJNBqk4=; 9, 61 -- b=O7I/2GHAhFuKBxOxVvF4JhzpDoFb3pLp/rZvqAxSthIuBaEss8/1gjxv/gxz5f0XrY 9, 61 -- KoiY25XpnnuVaSVmWAibsWWkORxnjdNyADngxUqs9Y3APKe4y8oHWk0fGMLA9NClOY1V 9, 61 -- XS+gI9dVQsAyHbs2XquWdJkUmiC0F1ubwgWoeXMxq5QxqkkBei6V854rjRsr9iYjxFxe 9, 61 -- /m/yjnJqcmnHwR7nfT5SZk9FBO8OJtuyKS81PAJ7a8MiPPalD1LnkqcyfrQztLTyKT++ 9, 61 -- mCJmQdzmX9tjKpeKuBQRbrL4P9Y5eXbTF2vBdesGBLxDJPI86cwHdJv5oWt8PSVOcknZ 9, 61 -- L7uw== 9, 61 -- X-Gm-Message-State: AN3rC/59qQcvuUdmn4iW+YS/KiY4OP/aS58Gl4RcWBBBqVi7e7M22m+g 9, 61 -- R8WwlaeX59UdTMeTFTKj4r2SMZgtBZFoodRBz1ggrYWmKQYnw5w1wbzKr8c6ZI8PKuol5hkthz9 9, 61 -- 14cSo22s45MbBIsfaAerhdegFLRlfA8Qzecy7 9, 61 -- X-Received: by 10.25.18.77 with SMTP id h74mr648048lfi.63.1493240412601; 9, 61 -- Wed, 26 Apr 2017 14:00:12 -0700 (PDT) 9, 61 -- X-Received: by 10.25.18.77 with SMTP id h74mr648036lfi.63.1493240412275; Wed, 9, 61 -- 26 Apr 2017 14:00:12 -0700 (PDT) 9, 61 -- MIME-Version: 1.0 9, 61 -- Received: by 10.46.5.71 with HTTP; Wed, 26 Apr 2017 14:00:11 -0700 (PDT) 9, 61 -- From: Aaron Barnes {barnesa-at-umn.edu} 9, 61 -- Date: Wed, 26 Apr 2017 16:00:11 -0500 9, 61 -- Message-ID: {CAEZZkLja1HXEMvhjgh=KkNr9gZdOa8K=NOk6wuFsoFzHv5K0mg-at-mail.gmail.com} 9, 61 -- Subject: Re: [Microscopy] Formalin 9, 61 -- To: Microscopy-at-microscopy.com 9, 61 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
Dear Jonathan (not knowing whether you got additional personal replies which were not sent via the public listserver):
it might depend:
Is that bottle of Formalin 'labelled$B!F(B ( e. g. adhesive label indicating the producing company like MERCK, Fisher or whatever company$B!D(B, content, cat.no, etc.etc. ) and has high percentage of CH2O or is it only a lower concentrated, "home-made$B!H(B formalin solution made from concentrated Formaldehyde solution? Has it been prepared with buffer or without buffer (= hydrous only) solution?
If the former: usually if there has been included a 'stabilisator$B!F(B (besides 10% MetOH usually calcium carbonate these days, in former days: "dolomite powder$B!H(B) the white precipitate originates possibly from the stabilizer;
If the latter: it might be part of stabilizer (if the solution had not been filtered when it was mixed up) OR it might be in fact deteriorating FA-oligo- polymers (as Aaron Barnes pointed out) or even precipitated phosphate or other ions (if the FA-solution was prepared with a buffer). In that case:
If it is only 1 bottle (1 L or eventually also 1 gallon) I would do the following:
Either filter or take out most of the solution by a pipet without disturbing the precipitate and use only for "normal$B!H(B Histology (as Aaron Barnes pointed out already). The problem arises here only that you don't know about the real concentration of the fixative.
Or: dispose of (according to your legal & national safety concepts) after "neutralizing$B!H(B (decomposing) the solution with e.g. concentrated NaOH (which decomposes FA by means of the CANIZARRO-reaction to produce formic acid (HCOOH) and methanol (CH3OH) . Since the blocking of free aldehyde groups (in fixation of tissues for ICH or even ultrastructural ICH/IC-chemistry ) is done with glycine, sodium-borohydride or even ammoniumchloride-solutions (or addition of these substances to FA-solution) you could try to deactivate the FA-solution prior to (+/- safe) disposal by converting F-aldehyde to alcohol(s). Also addition of bisulfite has been reported to be efficient. If you would like to look for other possibilities to dispose of old (perhaps deteriorated and of uncertain origin ) FA-solutions you might search by Googleing for: | formaldehyde OR formalin AND deactiv* AND disposal | (also perhaps read my blog on FA-safe disposal on: https://www.researchgate. net/post/How_do_you_neutralize_formaldehyde )
Another possibility: Aldehydes Many aldehydes are respiratory irritants, and some, such as formaldehyde and acrolein, are quite toxic. There is sometimes merit in oxidation of aldehydes to the corresponding carboxylic acids, which are usually less toxic and less volatile. Procedure for Permanganate Oxidation of 0.1 mol of Aldehyde 3RCHO + 2KMnO4 $B"*(B 2RCO2K + RCO2H + 2MnO2 + H2O A mixture of 100 mL of water and 0.1 mol of aldehyde is stirred in a 1-L round-bottomed flask equipped with a thermometer, dropping funnel, stirrer, steam bath, and, if the aldehyde boils below 100 $B!k(BC, a condenser. Approximately 30 mL of a solution of 12.6 g (0.08 mol, 20% excess) of potassium permanganate in 250 mL of water is added over a period of 10 minutes. If the temperature rises above 45 $B!k(BC, the solution should be cooled. If this addition is not accompanied by a rise in temperature and loss of the purple permanganate color, the mixture is heated by the steam bath until a temperature is reached at which the color is discharged. The rest of the permanganate solution is added slowly at within 10 $B!k(BC of this temperature. The temperature is then raised to 70 to 80 $B!k(BC, and stirring continued for 1 hour or until the purple color has disappeared, whichever occurs first. The mixture is cooled to room temperature and acidified with 6 N sulfuric acid. (CAUTION: Do not add concentrated sulfuric acid to permanganate solution because explosive manganese oxide (Mn2O7) may precipitate.) Enough solid sodium hydrogen sulfite (at least 8.3 g, 0.08 mol) is added with stirring at 20 to 40 $B!k(BC to reduce all the manganese, as indicated by loss of purple color and dissolution of the solid manganese dioxide. The mixture is washed down the drain with a large volume of water. If the aldehyde contains a carbon-carbon double bond, as in the case of the highly toxic acrolein, 4 mol (20% excess) of permanganate per mol of aldehyde is required to oxidize the alkene bond and the aldehyde group.
PROCEDURES FOR THE LABORATORY-SCALE TREATMENT OF SURPLUS AND WASTE CHEMICALS (from an article launched to web by University of Geneva and really helpful): Formaldehyde is oxidized conveniently to formic acid and carbon dioxide by sodium hypochlorite. Thus 10 mL of formalin (37% formaldehyde) in 100 mL of water is stirred into 250 mL of hypochlorite laundry bleach (5.25% NaOC1) at room temperature and allowed to stand for 20 minutes before being flushed down the drain. This procedure is not recommended for other aliphatic aldehydes because it leads to chloro acids, which are more toxic and less biodegradable than corresponding unchlorinated acids. https://www.unige.ch/sciences/chiorg/matile/12-ProceduresforLabTreatmentofWa steChemicals.pdf
Let us know how your problem was solved$B!D(B. Thank you, best regards, Wolfgang, Salzburg-Austria
Von: barnesa-at-umn.edu [mailto:barnesa-at-umn.edu] Gesendet: Mittwoch, 26. April 2017 23:10 An: wij.muss-at-aon.at Betreff: [Microscopy] Re: Formalin This post has been answered too to the Listserver
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Technically you can heat the solution to try and depolymerize the presumptive paraformaldehyde oligomers. I do *not* recommend trying this - it works occasionally, but hot formalin is almost never a good idea even in a hood.
Option 1: Send it to Haz Waste and buy another bottle. Option 2: Filter it and use it anyway.
I've needed to use option 2 a few times over the years - mostly due to reagent shipping issues and/or "surprise!" samples. In my hands, filtered PF works okay for straight histology-level work, so-so (at best) for immuno work, and leads to a range of....interesting results for EM samples.
I'd still recommend option 1 - formalin is { $10/liter from Fisher. In a pinch, filtering for H&E or other optical microscopy will probably be okay.
Aaron
----- Aaron Barnes, MD, PhD Clinical Pathology Resident (PGY1) | Dept of Lab Medicine & Pathology University of Minnesota Medical School Dunny Lab | Dept of Microbiology & Immunology
On Wed, Apr 26, 2017 at 3:33 PM, {jkrupp-at-deltacollege.edu} wrote: } ----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 26, 20 -- From wij.muss-at-aon.at Thu Apr 27 06:14:52 2017 26, 20 -- Received: from bsmtp1.bon.at (bsmtp1.bon.at [213.33.87.15]) 26, 20 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v3RBEpRS026066 26, 20 -- for {microscopy-at-microscopy.com} ; Thu, 27 Apr 2017 06:14:52 -0500 26, 20 -- Received: from MussTHINK (unknown [93.83.25.98]) 26, 20 -- by bsmtp1.bon.at (Postfix) with ESMTPSA id 3wDF4J0Vwxz5tlK; 26, 20 -- Thu, 27 Apr 2017 13:25:35 +0200 (CEST) 26, 20 -- From: "MUSS Wolfgang \(SCUR Board Member\)" {wij.muss-at-aon.at} 26, 20 -- To: {microscopy-at-microscopy.com} 26, 20 -- Cc: {barnesa-at-umn.edu} 26, 20 -- Subject: [Microscopy] Re: Formalin 26, 20 -- Date: Thu, 27 Apr 2017 13:25:36 +0200 26, 20 -- Message-ID: {001001d2bf48$fe5ddb00$fb199100$-at-aon.at} 26, 20 -- MIME-Version: 1.0 26, 20 -- Content-Type: text/plain; 26, 20 -- charset="iso-2022-jp" 26, 20 -- Content-Transfer-Encoding: 7bit 26, 20 -- X-Mailer: Microsoft Outlook 15.0 26, 20 -- Thread-Index: AdK/SJtGh5ndRM2MSdyk4loJwNDeUg== 26, 20 -- Content-Language: de ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both hexi-at-missouri.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: hexi-at-missouri.edu Name: Xiaoqing He
Organization: Electron microscopy core at University of Missouri
Title-Subject: [Filtered] Bruker EDS on Tecnai TEM
Message: Dear Listener,
I am wondering anyone on the list has a Bruker TEM acceptance checklist that you are kindly to share. Any feedback on the performance of Bruker EDS on FEI Tecnai TEM would be greatly appreciated!
Please send me email privately.
Thanks. Xiaoqing He
http://emc.missouri.edu/
Login Host: 161.130.188.220 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
a customer of mine has an ISO certification of the EM lab coming.
One of the problems is to reliably demonstrate the thickness of the 50nm UM slices and the semi-thick 500 nm slices in clinical workflow.
Normally this is done in the field by using the interference color of the slices in the water tray which is not very accurate and depends also on the color spectra of the illumination system, the resin system, the accuracy of the ultramicrotome, the user, room situation; what shall I say more...?
Did anyone of you had this problem and how did you solve it?
This question is also posed to the manufacturers of ultramicrotomes, RMC and LEICA. How do you handle this?
Did anyone measure the thickness of the slices and how? Through-focus-series in TEM? Some setup in SEM?
Best wishes,
Stefan
--
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Another thing to consider, is do you care about the thickness of the section (on water, grid, slide), or the thickness of the material removed from the block; i.e. the block advance (assuming the ultramicrotome is cutting all the material off after each advance).
For our studies, the block advance, which can be related back to the original volume of tissue, is what we like to know. If you minimise compression ( ultrasonic diamond knifes, different angles of knife and/or harder resin), then the block advance and the section thickness on grid begin to converge.
Best regards,
Ben
-- Research Support Manager (Imaging and IT Systems) MRC Brain Network Dynamics Unit, University of Oxford Department of Pharmacology, Mansfield Road, Oxford, OX1 3TH, United Kingdom. Telephone: 01865 271897 {http://www.mrcbndu.ox.ac.uk/}
-----Original Message----- X-from: stefan.diller-at-t-online.de [mailto:stefan.diller-at-t-online.de] Sent: 28 April 2017 09:33 To: Ben Micklem {ben.micklem-at-pharm.ox.ac.uk}
Stefan,
I thought the original method to determine section thickness accurately was to shadow sections, at a known angle with the sections on mica (or similar), and then calculate the height (thickness) of the seciton by the length of the shadowed metal deposit and the known angle. Admittedly, this is a lot of prep work and not something one would want to introduce into a "routine procedure".
Please correct me, gently, if I am wrong ;-)
Lorenzo
On 4/28/17, stefan.diller-at-t-online.de {stefan.diller-at-t-online.de} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear All, } } a customer of mine has an ISO certification of the EM lab coming. } } One of the problems is to reliably demonstrate the thickness of the 50nm UM } slices and the semi-thick 500 nm slices in clinical } workflow. } } Normally this is done in the field by using the interference color of the } slices in the water tray which is not very accurate and } depends also on the color spectra of the illumination system, the resin } system, the accuracy of the ultramicrotome, the user, room } situation; what shall I say more...? } } Did anyone of you had this problem and how did you solve it? } } This question is also posed to the manufacturers of ultramicrotomes, RMC and } LEICA. How do you handle this? } } Did anyone measure the thickness of the slices and how? Through-focus-series } in TEM? Some setup in SEM? } } } Best wishes, } } Stefan } } } -- } } } ----------------------------------------------------- } Stefan Diller - Scientific Photography } Arndtstrasse 22 } D - 97072 Wuerzburg Germany } ++49-931-7848700 Phone } ++49-931-7848701 Fax } ++49-175-7177051 Mobile } } Websites: } www.nanoflight.info } www.stefan-diller.com } www.electronmicroscopy.info } www.elektronenmikroskopie.info } www.zwillingsprojekt.de } www.assisi.de } Anfahrt: http://Mail.map24.com/Stefan.Diller } ----------------------------------------------------- } } } ==============================Original } Headers============================== } 16, 22 -- From stefan.diller-at-t-online.de Fri Apr 28 03:23:22 2017 } 16, 22 -- Received: from mailout11.t-online.de (mailout11.t-online.de } [194.25.134.85]) } 16, 22 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } v3S8NLwg023883 } 16, 22 -- for {microscopy-at-microscopy.com} ; Fri, 28 Apr 2017 03:23:21 -0500 } 16, 22 -- Received: from fwd25.aul.t-online.de (fwd25.aul.t-online.de } [172.20.26.130]) } 16, 22 -- by mailout11.t-online.de (Postfix) with SMTP id 27853421586C } 16, 22 -- for {microscopy-at-microscopy.com} ; Fri, 28 Apr 2017 10:34:09 +0200 } (CEST) } 16, 22 -- Received: from mac-pro.local } (Xje4XiZe8hUg5A+7b1H-RU5yxmCDfCxk2HhwMYiucqqUOhNNU6VdkajmIjgbvXDQHN-at-[93.203.66.207]) } by fwd25.t-online.de } 16, 22 -- with (TLSv1.2:ECDHE-RSA-AES256-SHA encrypted) } 16, 22 -- esmtp id 1d41Lm-1Prfu40; Fri, 28 Apr 2017 10:34:02 +0200 } 16, 22 -- To: "Microscopy-at-microscopy.com" {microscopy-at-microscopy.com} } 16, 22 -- From: Stefan Diller {stefan.diller-at-t-online.de} } 16, 22 -- Subject: Certified thickness of ultramicrotome cuts - advice } needed } 16, 22 -- Message-ID: {6140f078-b27e-6584-1786-33d9c9d26bae-at-t-online.de} } 16, 22 -- Date: Fri, 28 Apr 2017 10:34:02 +0200 } 16, 22 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; } rv:45.0) } 16, 22 -- Gecko/20100101 Thunderbird/45.8.0 } 16, 22 -- MIME-Version: 1.0 } 16, 22 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 16, 22 -- Content-Transfer-Encoding: 7bit } 16, 22 -- X-ID: } Xje4XiZe8hUg5A+7b1H-RU5yxmCDfCxk2HhwMYiucqqUOhNNU6VdkajmIjgbvXDQHN } 16, 22 -- X-TOI-MSGID: bc093b1a-2ed2-449a-b333-08f5c5090799 } ==============================End of - } Headers============================== }
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jfrechette-at-luc.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Operational Manager Position
Message: The Imaging Core Facility at Stritch School of Medicine, Loyola University Chicago, is currently looking for an Operational Manager to facilitate the day to day operations of the facility. The Operational Manager will work with the executive director of the facility to operate the imaging resources associated with the imaging facility. Key duties associated with this position are familiarity with sample preparation and data acquisition using Transmission Electron Microscopy sufficient to work with institutional investigators to incorporate TEM into their research.
Minimum Education: -Required: Bachelors Degree -Strongly Preferred: Masters Degree or Ph.D. Specific Degree(s): Molecular Biology, Biology, Biochemistry or Chemistry. Bachelor's prepared candidates must have experience equivalent to a master's program. Minimum Experience: -Required: 4-10 years of previous experience with EM sample preparation and data acquisition -Familiarity with fluorescence microscopy also preferred but not required. For more details, or to apply online please visit: www.careers.luc.edu/applicants/Central?quickFind=59639
Login Host: 147.126.51.4 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both bethrichardson-at-uga.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] support films for TEM - anyone making silicon nitride or graphene films?
Message: Hi all, I'd like to make good support films for TEM. I use to be able to make films without holes in them but my tried-and-true method using 0.25% Formvar in ethylene dichloride hasn't been working (purchased solution or made in-house). Holey support films have become a way of life and one of the weakest links in my productivity. I'd like to learn more about making silicon nitride or graphene films (they are so expensive to buy). Can anyone share a protocol? If necessary I have access to a nice chemist and a clean room. thanks in advance for any advice, Beth
Login Host: 198.137.20.215 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
Work has been done to determine section thickness in a more exact way than using interference colors alone.
One method is to use the minimal folds method using the TEM (Small, 1968). Imagine crinkling a piece of paper so a fold forms in the middle in the Z axis, which will be twice the thickness of the paper. This fold can be measured on the TEM to get a relatively accurate thickness measurement. Ideally, measure multiple folds and get an average thickness. This method is addressed nicely in the following paper:
When using samples with mitochondria, you can also use the cylindrical shape of those to estimate section thickness (Fiala and Harris, 2001). This is ideal if you have sections with very few wrinkles and was found to have an average thickness measurement very similar to that of the minimal folds method.
Yet another method is to reembed some sections you've cut and section them again at 90 degrees to measure the thickness (Bedi, 1987).
Finally, laser confocal microscopes can be used to measure the section thickness with a reported accuracy of 1 nm (see Kubota et al. 2009).
Hope this helps,
Connon
Connon Thomas Max Planck Florida Institute for Neuroscience Electron Microscopy Technician One Max Planck Way Jupiter, FL 33458 Email: Connon.Thomas-at-mpfi.org
Formalin is historically ~40% formaldehyde in water and laced with 5-10% MeOH to inhibit polymerization(?). Thus, 10% "Formalin" is 4% in Formaldehyde. That is the historically recommended concentration of the gas as a fixative. 4% paraformaldehyde at 4DegC (buffered or not) is also a very good treatment (10-20 min) for 'freezing,' muscle in thin tissues that are under various degrees of tension (e.g., in a partially to maximally distended urinary bladder), before they are immersed (or filled) with with the same fixative. Fixation at 4DegC is a very good fixative for both routine and special histology.
I stopped using Formalin in the mid 1970's, and substituted as follows.
I usually make 20% HCHO from Paraformaldehyde, and I can refrigerate it in 100ml aliquots for over a decade without any polymerization.
Formalin ought not be used for anything in biological histology, unless the MeOH is absent - which wolution should not be called "Formalin."
Cheers,
Fred Monson
Frederick C. Monson, PhD Technical Director - until the end of 16 June, 2017. Center for Microanalysis and Imaging, Research and Training (CMIRT) West Chester University of PA Geology-Astronomy 750 South Church St. West Chester, PA, 19383 fmonson-at-wcupa.edu 610-738-0437(Work)
-----Original Message----- X-from: barnesa-at-umn.edu [mailto:barnesa-at-umn.edu] Sent: Wednesday, April 26, 2017 5:01 PM To: Monson, Frederick {FMonson-at-wcupa.edu}
Technically you can heat the solution to try and depolymerize the presumptive paraformaldehyde oligomers. I do *not* recommend trying this - it works occasionally, but hot formalin is almost never a good idea even in a hood.
Option 1: Send it to Haz Waste and buy another bottle. Option 2: Filter it and use it anyway.
I've needed to use option 2 a few times over the years - mostly due to reagent shipping issues and/or "surprise!" samples. In my hands, filtered PF works okay for straight histology-level work, so-so (at best) for immuno work, and leads to a range of....interesting results for EM samples.
I'd still recommend option 1 - formalin is { $10/liter from Fisher. In a pinch, filtering for H&E or other optical microscopy will probably be okay.
Aaron
----- Aaron Barnes, MD, PhD Clinical Pathology Resident (PGY1) | Dept of Lab Medicine & Pathology University of Minnesota Medical School Dunny Lab | Dept of Microbiology & Immunology
On Wed, Apr 26, 2017 at 3:33 PM, {jkrupp-at-deltacollege.edu} wrote: } } } } ---------------------------------------------------------------------- } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } I have a bottle of Formalin that has a white precipitate, probably polymerized formaldehyde from being stored in the refrigerator. } } Is there anything I can do to return the solution to normal? } } Thanks } } Jon } } Jonathan Krupp } ASB&T } San Joaquin Delta College } 5151Pacific Ave. } Stockton, CA 95207 } (209) 954-5284 } jkrupp-at-deltacollege.edu } }
From devlinrobb1-at-gmail.com Fri Apr 28 21:19:42 2017 Return-Path: {devlinrobb1-at-gmail.com} Received: from gmail.com ([218.232.94.109]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v3T2Jd46032327 for {microscopylistserverarchive6-at-microscopy.com} ; Fri, 28 Apr 2017 21:19:41 -0500 Message-ID: {C0874B91.A59EA789-at-gmail.com}
} On Apr 28, 2017, at 10:06 AM, microscopy.listserver-at-gmail.com wrote: } } I'd like to make good support films for TEM. I use to be able to make films without holes in them } but my tried-and-true method using 0.25% Formvar in ethylene dichloride hasn't been working } (purchased solution or made in-house).
Dear Beth, I cannot respond to the other films mentioned in your post, but as to formvar, ethylene dichloride decomposes to release HCl, which impairs formvar films, so be sure your—or your formvar supplier’s—is fresh. Also, it has been my experience that high-humidity environments make film-making problematic, and GA can be very humid this time of year. Yours, Bill
==============================Original Headers============================== 7, 34 -- From wtivol-at-sbcglobal.net Sun Apr 30 18:55:11 2017 7, 34 -- Received: from nm26-vm9.access.bullet.mail.bf1.yahoo.com (nm26-vm9.access.bullet.mail.bf1.yahoo.com [216.109.115.216]) 7, 34 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v3UNtBfL030131 7, 34 -- for {microscopy-at-microscopy.com} ; Sun, 30 Apr 2017 18:55:11 -0500 7, 34 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=sbcglobal.net; s=s2048; t=1493597169; bh=Xquvjf67Apc0e1OhOi2vjKbaYmkEa2BhgS1qimsjRqU=; h=Subject:From:In-Reply-To:Date:References:To:From:Subject; b=JyKYEp1FufbAwhFzpR4SKX2lnOnbmBho1/KAjCTDE/+WvDvbytcElXbIl5pN+YfHcoJhll3BFWJZj3PHtrFV615jb7yjMfbHuu05nHi4aNa3ZA/Sxq5fvakffXkzRjaEItpD4mU5DpcvSgBT9FCeLOZNA3J41zInqnGUnyQdFwr/civR+e+V1C1AWbLCG/7+1bYu4SbGWMRwONJtQUESPfH41+DFmRlcR6ejq9PUcWONbcrEJWm++RJ/kRgpPJXK2uShuT38RwBVJzcEV+kH01gEcsasONWiVeYq+RGOfxZ8lwHR7IhABTsItbptvNE7/GpGEAZXM1bixJrbS0CTJQ== 7, 34 -- Received: from [66.196.81.160] by nm26.access.bullet.mail.bf1.yahoo.com with NNFMP; 01 May 2017 00:06:09 -0000 7, 34 -- Received: from [98.138.104.97] by tm6.access.bullet.mail.bf1.yahoo.com with NNFMP; 01 May 2017 00:06:09 -0000 7, 34 -- Received: from [127.0.0.1] by smtp117.sbc.mail.ne1.yahoo.com with NNFMP; 01 May 2017 00:06:09 -0000 7, 34 -- X-Yahoo-Newman-Id: 256218.86480.bm-at-smtp117.sbc.mail.ne1.yahoo.com 7, 34 -- X-Yahoo-Newman-Property: ymail-3 7, 34 -- X-YMail-OSG: kyxHpt4VM1nbfHracwDUT3H.Ct.u.OLeW.2NN.IeSmUaHcQ 7, 34 -- 61eK8wg5llQsuuFsSSNnRFFrsaMU7wRc4WszxRL2F0zckRv527owlBalEy1C 7, 34 -- aZkWmX0Zs1CiEip8WhbbWfgz2HEokV964QwX_Fp0J_Sg2GZhMFx.DNPRkRTJ 7, 34 -- io6ufzMlvNgKXcR0GUyhjfR4xRFQulmjeEm6YXDEYI10Ihj9lABxOMNEosHy 7, 34 -- mxLBLcdwPRQRcDIbF2Ay7y3msRxe.b9AX2jUsxnfAKBQIZYuuokJzVvooov9 7, 34 -- Adn_fCtPpW1QHqCi7brkW3_9oieohuL5UJhjPVQd_3S2uj0IRuJI7nwrSZqk 7, 34 -- 2aSb8pLJMUB2uGIDMYKu2_JewRulxBCXvxUhF3B9UFq60qyPgbT49nPpD7Yf 7, 34 -- SARY3jsX.e1styTreCu5.kbuFdHZqTPrDDfhy84axYOvAztJrUUOaouWkrVC 7, 34 -- tzbkP96GT77nZhdBVqk2UsP1f3K2QdJV_awIVeJdWgI0lpm8ZEPSSr6RHgQN 7, 34 -- nFGdyZ8M0aNPTEO67TpgHG31ET3jizoYqa09bD02cs.OImTSycras.y3fq_3 7, 34 -- gdv_I 7, 34 -- X-Yahoo-SMTP: 2C4lFAKswBB2zWDtHIVLYPvHWQTcLYoM2wWnLTebSN_t 7, 34 -- Content-Type: text/plain; charset=utf-8 7, 34 -- Mime-Version: 1.0 (Mac OS X Mail 9.3 \(3124\)) 7, 34 -- Subject: Re: [Microscopy] viaWWW:support films for TEM - anyone making silicon nitride or 7, 34 -- From: Bill & Sue Tivol {wtivol-at-sbcglobal.net} 7, 34 -- In-Reply-To: {201704281706.v3SH6IrZ020583-at-microscopy.com} 7, 34 -- Date: Sun, 30 Apr 2017 17:06:07 -0700 7, 34 -- Message-Id: {415CF08B-1591-4604-A228-1BB1141A9247-at-sbcglobal.net} 7, 34 -- References: {201704281706.v3SH6IrZ020583-at-microscopy.com} 7, 34 -- To: bethrichardson-at-uga.edu, microscopy-at-microscopy.com 7, 34 -- X-Mailer: Apple Mail (2.3124) 7, 34 -- Content-Transfer-Encoding: 8bit 7, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v3UNtBfL030131 ==============================End of - Headers==============================
The Central Facility for Advanced Microscopy and Microanalysis (CFAMM) at the University of California at Riveside has an open position for electron microscopy laboratory technician. Position responsibilities include operation and routine maintenance of the electron microscopes, FIB, ancillary equipment, and all phases of sample preparation and analysis of various types of materials and tissue. This position includes assisting and training facility users in specimen preparation, instrument operation, and related techniques.
We are looking to purchase a used (but working) Reichert-Jung Model FC-4E cryo stage and controller for our Ultracut E ultramicrotome. Please let me know if you have one you are interested in selling.
Thank you,
Melissa Holman Laboratory Manager 3300 Breckinridge Blvd Suite 400 Duluth, GA 30096 770.662.8509
Visit us at: www.mvainc.com Connect with me on: LinkedIn
--------------------------------------------------------------------------------------------------------------------------- The information in this email is confidential and may be legally privileged. It is intended solely for the addressee. If you are not the intended recipient, please delete the email and notify MVA Scientific Consultants of the transmission error.
From voraisai2-at-gmail.com Tue May 2 23:49:54 2017 Return-Path: {voraisai2-at-gmail.com} Received: from gmail.com ([182.16.57.234]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v434nqVf029539 for {microscopylistserverarchive6-at-microscopy.com} ; Tue, 2 May 2017 23:49:53 -0500 Message-ID: {901CE4DE.5C2255A2-at-gmail.com}
X-from: stefano-at-soquelec.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both stefano-at-soquelec.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] TEM open position at INRS near Montreal, QC: Research Assistant in ultrafast TEM
Message: OPEN RESEARCH-ASSISTANT POSITION Institut National de la Recherche Scientifique (INRS) Université du Québec Centre Énergie Matériaux Télécommunications at Varennes, Canada
INRS-EMT is undertaking a unique project to image materials with combined spatial and temporal resolutions that were not possible before. In this context, INRS will hire a full-time research assistant to develop, use and maintain several instruments that are housed in the new Infrastructure for Advanced Imaging (IAI). These instruments include: modified transmission electron microscopes (TEM), sample preparation tools and pulsed lasers. In addition to these duties, the research assistant is expected to train users and manage the facility at the user level. The assistant will also have opportunities to participate to the ongoing academic research in IAI. INRS will provide all the necessary trainings to the research assistant to use and maintain the equipment. Therefore, candidates with enthusiasm to learn new technologies are particularly encouraged to apply.
The research assistant will report to the professor responsible for the Infrastructure for Advanced Imaging.
Requirements
Education Minimum of a Bachelors degree in Physics, Applied and Engineering Physics, Materials Science, Electrical Engineering, and related disciplines. Experience Experience with transmission electron microscopes, lasers and optical components, and sample preparation instruments. Other Ability and enthusiasm to work in a research team. Good communication skills.
Location
Institut national de la recherche scientifique Centre Énergie Matériaux Télécommunications 1650, boulevard Lionel-Boulet Varennes (Québec) J3X 1S2 (work location is 30 kms away from downtown Montréal)
Salary/Benefits
The salary and benefits will be determined according to the qualifications of the candidate. How to Apply
Interested candidates should apply by submitting their CV online (http://www.inrs.ca/english/career-opportunities). Further information can be obtained by writing to: recrutement-at-adm.inrs.ca.
INRS is a graduate level university active in fundamental and applied research. It brings together 150 professors and nearly 700 graduate-level students and postdoctoral fellows in four centers located in Quebec City, Montréal, Varennes and Laval. It is consistently ranked as one of the top universities in Canada in terms of research intensity (grants per professors). The Infrastructure for Advanced Imaging is a $15M project funded by the Canadian and Quebec governments through the Canadian Foundation for Innovation. INRS is committed to employment equality program. The University invites women, visible minorities, ethnic minorities, Aboriginal people and persons with disabilities to apply.
Login Host: 207.81.2.16 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
We would like to draw your attention to the 2017 Yale Microscopy Workshop.
We invite you to participate in a two day workshop. This free annual event is a combination of symposium, demonstrations, technical lectures and small group practicals. It is open to the international research community and only requires your free registration.
Time: June 6 - 7th, 2017 Location: Yale University School of Medicine, The Anlyan Center (TAC), New Haven, CT Cost: Free on-line or on-site registration Information: https://medicine.yale.edu/lab/microscopy/ {http://microscopy.med.yale.edu/index.aspx} {https://medicine.yale.edu/lab/microscopy/%3Chttp://microscopy.med.yale.edu/index.aspx%3E}
Features: - Symposia on image analysis and high-dimensional imaging - Concurrent workshop on correlative live-cell STED and cryo-electron microscopy, and cryo EM techniques - Demonstrations, technical lectures, and small group practicals.
Please see the website for free registration, list of accessible equipment, and the schedule of events. Additional technical lectures are likely to be added soon. http://medicine.yale.edu/lab/microscopy/schedule/
We look forward to seeing you there,
The organizers, Ann Haberman ann.haberman-at-yale.edu {mailto:ann.haberman-at-yale.edu} Derek Toomre derek.toomre-at-yale.edu {mailto:derek.toomre-at-yale.edu} Joerg Bewersdorf joerg.bewersdorf-at-yale.edu {mailto:joerg.bewersdorf-at-yale.edu} Xinran Liu xinran.liu-at-yale.edu {mailto:xinran.liu-at-yale.edu}
From sawijama5-at-gmail.com Wed May 3 21:01:18 2017 Return-Path: {sawijama5-at-gmail.com} Received: from gmail.com (mail.dongnamdc.com [222.97.39.100] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v4421BEp031177 for {microscopylistserverarchive6-at-microscopy.com} ; Wed, 3 May 2017 21:01:13 -0500 Message-ID: {7649EAE9.693E3010-at-gmail.com}
I was looking at some pond water in a light microscope, and managed to capture this guy. Being a physicist, I have absolutely no idea what it might be, but I’m pretty sure that someone on this list will know what it is.
Here is a video that I took of it: http://www.jkraft.net/unknown-microbe.mp4
Also, if anyone knows of a good internet source of information on how to identify the random little slimy bits in pond water, I would appreciate it.
Thanks in advance,
Justin A. Kraft
==============================Original Headers============================== 5, 47 -- From kraftpiano-at-gmail.com Thu May 4 19:22:01 2017 5, 47 -- Received: from mail-qt0-f196.google.com (mail-qt0-f196.google.com [209.85.216.196]) 5, 47 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v450M1nU026775 5, 47 -- for {microscopy-at-microscopy.com} ; Thu, 4 May 2017 19:22:01 -0500 5, 47 -- Received: by mail-qt0-f196.google.com with SMTP id a46so384938qte.0 5, 47 -- for {microscopy-at-microscopy.com} ; Thu, 04 May 2017 17:33:14 -0700 (PDT) 5, 47 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 5, 47 -- d=gmail.com; s=20161025; 5, 47 -- h=from:content-transfer-encoding:mime-version:subject:message-id:date 5, 47 -- :to; 5, 47 -- bh=jottZit2bdx8/O5Ck+HDjHfzCIAiTsZCrsd9ZJ9p5TI=; 5, 47 -- b=Uoftj1aDCCE2u5BN/zh75w28QEqU3dngsp1Uyu//NmIQhO47Pih1FimMyKp6IXaUMq 5, 47 -- fCaa741xU8lkRVi97Q8UH4S+LLrYCyiv/wrPWecVKiws9lJ+gCXTFZW3wW6+ucbiWJcQ 5, 47 -- WsHHMV6am08/zTvHHYe3uou8r1fdbwW3pNKq9777oaYZji8xFramLwxbfs8N/8J7UGUp 5, 47 -- sZKf1LakCqOvv3KLVE05RR1D8jABM3CgtYn+EcOh1RiUOVNfdLf2hjfsSfkPevZEcZr2 5, 47 -- Y1EjYkDcuC6LlysSugExyr2z1X5oNRtxJc35HNH18CO6N5UEsPrEAsl2fQrrp0zD8Qwq 5, 47 -- Rmrw== 5, 47 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 5, 47 -- d=1e100.net; s=20161025; 5, 47 -- h=x-gm-message-state:from:content-transfer-encoding:mime-version 5, 47 -- :subject:message-id:date:to; 5, 47 -- bh=jottZit2bdx8/O5Ck+HDjHfzCIAiTsZCrsd9ZJ9p5TI=; 5, 47 -- b=SO0vFB7npCdoThmmEdaBpBENsVYlFZwRCgN0wbkeBp7RUM6C4tOURD0gMmmjpMYabC 5, 47 -- Ak9ZBydf3vmUW6YguBTCNF6zO8FTEP/4Hlu79riVI7IDXgRsu1OnvT3izVmPS3pYpIcx 5, 47 -- zcKt0RBDyXGZwnPCNbyrSIgXlEdxUYX6YuiyFsWt6AeCl82UDczYG5WgJBeWlFathIqg 5, 47 -- 0J63ygyl8Rfa5mQ2uuCV3n0V45vtDSsdVhH+5laWC2NICbC8PVGPoKWX04Q+BkkjepQ4 5, 47 -- 2WStSSRVNt/3bLXilKkObz862hdyB6nzo4JT9/UBk5zGY27u9JRwXL+IXy5pU37W6x7n 5, 47 -- RZHQ== 5, 47 -- X-Gm-Message-State: AN3rC/6PTVUH8X/2gR7+Hzc9ivUdGNiQCW2yq7TcGq8qrDmdS+w7uWCN 5, 47 -- 6cyn954FGAvGR9SZJ6I= 5, 47 -- X-Received: by 10.200.55.86 with SMTP id p22mr37680511qtb.167.1493944393819; 5, 47 -- Thu, 04 May 2017 17:33:13 -0700 (PDT) 5, 47 -- Received: from ?IPv6:2604:6000:7480:3200:6129:54d9:2011:85d5? ([2604:6000:7480:3200:6129:54d9:2011:85d5]) 5, 47 -- by smtp.gmail.com with ESMTPSA id s31sm2373511qtb.64.2017.05.04.17.33.13 5, 47 -- for {microscopy-at-microscopy.com} 5, 47 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 5, 47 -- Thu, 04 May 2017 17:33:13 -0700 (PDT) 5, 47 -- From: Justin Kraft {kraftpiano-at-gmail.com} 5, 47 -- Content-Type: text/plain; charset=utf-8 5, 47 -- Mime-Version: 1.0 (Mac OS X Mail 10.3 \(3273\)) 5, 47 -- Subject: Unknown microbe- does anyone know what this is? 5, 47 -- Message-Id: {B98D49CE-15AE-4C3B-B015-407AEAFD9FC1-at-gmail.com} 5, 47 -- Date: Thu, 4 May 2017 20:33:10 -0400 5, 47 -- To: microscopy-at-microscopy.com 5, 47 -- X-Mailer: Apple Mail (2.3273) 5, 47 -- Content-Transfer-Encoding: 8bit 5, 47 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v450M1nU026775 ==============================End of - Headers==============================
Contact the folks in Seth Tyler's lab at University of Maine – they run a "global worming" website about metazoan microfauna, but they're mostly marine. http://globalworming.umaine-biology.net/ Pennak's "Freshwater Invertebrates of North America" is a good reference. After that, you need to get into the literature for each group, or google the group name, then click "images".
But. Squished by the coverslip and without a scale, it's hard to tell. The squishedness prevents the critter from exhibiting its true form. There are several sets of somethings … 2 near the anterior end, just behind the mouth (on the right), one about ½ way along the body, on top of the animal and hard to see (about where the gut makes the sharp bend), and another 2 spaced equally behind this, on the "top" of the animal as it sits. This makes me think it's a seriously uncomfortable tardigrade. Too flattened by the coverslip to really show its true form. Common animals, but you need to put feet on your coverslips to give critters like this some room.
Great darkfield subjects as well as DIC. Through in a green filter below your condenser, and use the 40X DIC annulus with the 10X objective, and you'll have darkfield (100X annulus and 20/40X objective will work on some 'scopes).
Given how poorly studied these groups are, it could be a new species.
Phil ------------- Philip Oshel Imaging Facility Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576 office (989) 774-7567 lab
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
I was looking at some pond water in a light microscope, and managed to capture this guy. Being a physicist, I have absolutely no idea what it might be, but I’m pretty sure that someone on this list will know what it is.
Here is a video that I took of it: http://www.jkraft.net/unknown-microbe.mp4
Also, if anyone knows of a good internet source of information on how to identify the random little slimy bits in pond water, I would appreciate it.
Thanks in advance,
Justin A. Kraft
==============================Original Headers============================== 15, 54 -- From oshel1pe-at-cmich.edu Fri May 5 07:45:58 2017 15, 54 -- Received: from NAM03-BY2-obe.outbound.protection.outlook.com (mail-by2nam03on0113.outbound.protection.outlook.com [104.47.42.113]) 15, 54 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v45Cjvxs014918 15, 54 -- for {microscopy-at-microscopy.com} ; Fri, 5 May 2017 07:45:57 -0500 15, 54 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 15, 54 -- d=CentralMichigan.onmicrosoft.com; s=selector1-cmich-edu; 15, 54 -- h=From:Date:Subject:Message-ID:Content-Type:MIME-Version; 15, 54 -- bh=D8LooICBpda2JomoADXvnxmwSPUN4NYl1xevmj7sh7Y=; 15, 54 -- b=bI/aqeKWO9DQLdlPevihrJqHEsEF8tKiUyrWGd8c8BAF8Y0fL3zl5IhXnTcKks9hH/BGOq6TXd1MxiwLDGdyNlYeR0xSEhpVfMMK7K9wFzNdKqJ1UAdfEovSXm3bkQc2GriGIO44dyfNPHVrhaY4KF1KWMQ3Cn1dlLFOhCNgwT8= 15, 54 -- Received: from DM2PR05MB768.namprd05.prod.outlook.com (10.141.179.27) by 15, 54 -- DM2PR05MB765.namprd05.prod.outlook.com (10.141.179.14) with Microsoft SMTP 15, 54 -- Server (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_128_CBC_SHA256_P256) id 15, 54 -- 15.1.1075.1; Fri, 5 May 2017 12:57:10 +0000 15, 54 -- Received: from DM2PR05MB768.namprd05.prod.outlook.com ([10.141.179.27]) by 15, 54 -- DM2PR05MB768.namprd05.prod.outlook.com ([10.141.179.27]) with mapi id 15, 54 -- 15.01.1075.010; Fri, 5 May 2017 12:57:10 +0000 15, 54 -- From: "Oshel, Philip Eugene" {oshel1pe-at-cmich.edu} 15, 54 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 15, 54 -- Subject: Re: [Microscopy] Unknown microbe- does anyone know what this is? 15, 54 -- Thread-Topic: [Microscopy] Unknown microbe- does anyone know what this is? 15, 54 -- Thread-Index: AQHSxTmBRkoGh6R0DEW6nP+JnAmmKaHlcLMA 15, 54 -- Date: Fri, 5 May 2017 12:57:09 +0000 15, 54 -- Message-ID: {5C8FADC6-D17F-4351-8107-3E3FD7F38C2A-at-cmich.edu} 15, 54 -- References: {201705050038.v450cbBB011248-at-microscopy.com} 15, 54 -- In-Reply-To: {201705050038.v450cbBB011248-at-microscopy.com} 15, 54 -- Accept-Language: en-US 15, 54 -- Content-Language: en-US 15, 54 -- X-MS-Has-Attach: 15, 54 -- X-MS-TNEF-Correlator: 15, 54 -- authentication-results: microscopy.com; dkim=none (message not signed) 15, 54 -- header.d=none;microscopy.com; dmarc=none action=none header.from=cmich.edu; 15, 54 -- x-originating-ip: [141.209.133.243] 15, 54 -- x-ms-publictraffictype: Email 15, 54 -- x-microsoft-exchange-diagnostics: 1;DM2PR05MB765;7:JtUfVI1hsfjUwzFaOo6y/8tpOvQv30hJ0WxXmyHaEfna5uQQgouIOUsrVZeKJ92AUcdXevt4JF+kbxhtoYdpjvCygdI7Wnjy0PhTTxU5xw+mvUhVpA8nUs91axOV4Soh/BmBjO+laufJ9rSa1QoB1qz1Q4rfbinfWuvpyt3PUApXZolfyBlPJBDyNYy8bpULNb7M7UjezH/taHlaAS4lzgz+OrNsNRnjzgP20vKMPefb/NnaRrL//d3GCbACfM56wMGEe4G5jwyOPRps3bQP6lf9Yc6WuNMYYI3IkyD7ekWeHJIe/0o+fNh9fR9rkp1vNm/5jsIgiw80A5vqDp3g+w== 15, 54 -- x-ms-office365-filtering-correlation-id: 58f07f42-90e5-46d3-89ea-08d493b63e12 15, 54 -- x-microsoft-antispam: UriScan:;BCL:0;PCL:0;RULEID:(22001)(2017030254075)(201703131423075)(201703031133081);SRVR:DM2PR05MB765; 15, 54 -- x-microsoft-antispam-prvs: {DM2PR05MB76581CE43C40FD7E11A125295EB0-at-DM2PR05MB765.namprd05.prod.outlook.com} 15, 54 -- x-exchange-antispam-report-test: UriScan:; 15, 54 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:(11241501159)(6040450)(601004)(2401047)(5005006)(8121501046)(10201501046)(93006095)(93001095)(3002001)(6041248)(20161123564025)(20161123560025)(20161123558100)(20161123555025)(201703131423075)(201702281529075)(201702281528075)(201703061421075)(201703061406153)(20161123562025)(6072148);SRVR:DM2PR05MB765;BCL:0;PCL:0;RULEID:;SRVR:DM2PR05MB765; 15, 54 -- x-forefront-prvs: 02981BE340 15, 54 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10019020)(6009001)(39450400003)(39400400002)(39410400002)(39850400002)(39840400002)(51874003)(189998001)(66066001)(6116002)(102836003)(75432002)(3846002)(7736002)(305945005)(36756003)(86362001)(122556002)(50986999)(81166006)(8936002)(54356999)(2351001)(76176999)(5660300001)(8676002)(1730700003)(82746002)(33656002)(2900100001)(6246003)(110136004)(83716003)(38730400002)(229853002)(2501003)(478600001)(966004)(1720100001)(25786009)(6436002)(5640700003)(88552002)(3660700001)(99286003)(6512007)(6506006)(77096006)(2950100002)(6916009)(6486002)(53936002)(3280700002)(2906002)(6306002);DIR:OUT;SFP:1102;SCL:1;SRVR:DM2PR05MB765;H:DM2PR05MB768.namprd05.prod.outlook.com;FPR:;SPF:None;MLV:sfv;LANG:en; 15, 54 -- spamdiagnosticoutput: 1:99 15, 54 -- spamdiagnosticmetadata: NSPM 15, 54 -- Content-Type: text/plain; charset="utf-8" 15, 54 -- Content-ID: {903E0237E6FAB7409A76B0B7255A6379-at-namprd05.prod.outlook.com} 15, 54 -- MIME-Version: 1.0 15, 54 -- X-OriginatorOrg: cmich.edu 15, 54 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 05 May 2017 12:57:09.8664 15, 54 -- (UTC) 15, 54 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted 15, 54 -- X-MS-Exchange-CrossTenant-id: c871bc6e-7cc6-4a57-a4eb-22309fc34963 15, 54 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: DM2PR05MB765 15, 54 -- Content-Transfer-Encoding: 8bit 15, 54 -- X-MIME-Autoconverted: from base64 to 8bit by microscopy.com id v45Cjvxs014918 ==============================End of - Headers==============================
Looks like it may be a freshwater Oligochaete, a type of Annelid worm (think earthworms). Couldn't tell you what species it is though, that would take some careful keying as there are hundreds of them in North America (if that's where you are!). If you look closely, it has what are called chaetae (bristles or hairs) in bunches on its body. There are quite a few keys to freshwater creatures, here are just a few--
Stroud Water Research Center Identification Guide to Freshwater Macroinvertebrates (a nice, very basic visual guide to more common groups): http://www.stroudcenter.org/education/MacroKey_Complete.pdf
Guide to the Freshwater Aquatic Microdrile Oligochaetes of North America (more specific to these worms): http://www.dfo-mpo.gc.ca/Library/33909.pdf
Guide to freshwater microorganisms: https://www.msnucleus.org/watersheds/mission/plankton.pdf
Freshwater Macroinvertebrates of Northeastern North America (A book I used in my undergrad to identify slimy bits in pond water) https://www.amazon.com/Freshwater-Macroinvertebrates-Northeastern-North-America/dp/0801496888/ref=pd_lpo_sbs_14_t_1?_encoding=UTF8&psc=1&refRID=GE75176NY822DJV8BM3Y
As was previously stated, it may be helpful to reach out to a freshwater invertebrate lab if you are still curious. Hope this helps!!!!
Cheers,
Connon Thomas Max Planck Florida Institute for Neuroscience Electron Microscopy Technician One Max Planck Way Jupiter, FL 33458 Email: Connon.Thomas-at-mpfi.org
==============================Original Headers============================== 11, 50 -- From Connon.Thomas-at-mpfi.org Fri May 5 08:41:26 2017 11, 50 -- Received: from NAM03-CO1-obe.outbound.protection.outlook.com (mail-co1nam03on0139.outbound.protection.outlook.com [104.47.40.139]) 11, 50 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v45DfQeF006884 11, 50 -- for {Microscopy-at-microscopy.com} ; Fri, 5 May 2017 08:41:26 -0500 11, 50 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=mpfi.org; s=selector1; 11, 50 -- h=From:Date:Subject:Message-ID:Content-Type:MIME-Version; 11, 50 -- bh=ORgYcY/osWefAIiauDtoRRRu+3K0xFsZVS/wEa/RT0w=; 11, 50 -- b=mONWQpdTdtWtTynswuhdzvfAyAeNnLfNmiIwtwrxwe5pWuvt/DsQ02ZLaUy6TsIwPTI4/GeMDrYpPYxj60vegov/PfXn6Ckf3Hqu12l/s8MbygP1JM+N5m8UWwhZfJCRgP/wo4Dc7Fz1DnWqw3pbTdWGARxAM59mTTqMcvRFKWs= 11, 50 -- Received: from BLUPR03MB520.namprd03.prod.outlook.com (10.141.80.154) by 11, 50 -- BLUPR03MB518.namprd03.prod.outlook.com (10.141.80.143) with Microsoft SMTP 11, 50 -- Server (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_128_CBC_SHA256_P256) id 11, 50 -- 15.1.1061.12; Fri, 5 May 2017 13:52:38 +0000 11, 50 -- Received: from BLUPR03MB520.namprd03.prod.outlook.com ([10.141.80.154]) by 11, 50 -- BLUPR03MB520.namprd03.prod.outlook.com ([10.141.80.154]) with mapi id 11, 50 -- 15.01.1061.022; Fri, 5 May 2017 13:52:37 +0000 11, 50 -- From: "Connon.Thomas-at-mpfi.org" {Connon.Thomas-at-mpfi.org} 11, 50 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 11, 50 -- Subject: Re: Unknown microbe- does anyone know what this is? 11, 50 -- Thread-Topic: Unknown microbe- does anyone know what this is? 11, 50 -- Thread-Index: AQHSxaO9oUMWalXB/kiu558InoQxlA== 11, 50 -- Date: Fri, 5 May 2017 13:52:37 +0000 11, 50 -- Message-ID: {BLUPR03MB5200E82E9FF0C713572A56C93EB0-at-BLUPR03MB520.namprd03.prod.outlook.com} 11, 50 -- Accept-Language: en-US 11, 50 -- Content-Language: en-US 11, 50 -- X-MS-Has-Attach: 11, 50 -- X-MS-TNEF-Correlator: 11, 50 -- authentication-results: microscopy.com; dkim=none (message not signed) 11, 50 -- header.d=none;microscopy.com; dmarc=none action=none header.from=mpfi.org; 11, 50 -- x-originating-ip: [25.162.191.4] 11, 50 -- x-ms-publictraffictype: Email 11, 50 -- x-microsoft-exchange-diagnostics: 1;BLUPR03MB518;7:BDpwnQf5oxddJNMfABT49e7kARysxv0jdweLsF41s0VnkqsLKWWPhDEE7R7RoiwjcdnTinYn5ndy6g1hKO/fOeEZTUGK3FTXODh1GuWuCtU5UJf3BWvr/kGPOvY2pKBSLs0vpVyVaSgm/UaPdDUB7bSDgcp4SvK3oEwrTke0UZIJxnbT3mYFbWAbwrWBlNLOkAjRW34PEhcynu0DUf0y5gAAgy/2N4yjjUpnMG+mVO3Ek0Il3qVZ4DdkKGue5xPR8vB5hmmo67Ej5EsUES118q2mdQdQtzWACzg8YjkEg14t0OLEJdcWvLpMGWuy9l3g4EnXy4MZ3ujk2vSTFY5fVg== 11, 50 -- x-ms-office365-filtering-correlation-id: dc242d0e-58ee-4b6a-33ed-08d493bdfd91 11, 50 -- x-microsoft-antispam: UriScan:;BCL:0;PCL:0;RULEID:(22001)(2017030254075)(201703131423075)(201703031133081)(201702281549075);SRVR:BLUPR03MB518; 11, 50 -- x-microsoft-antispam-prvs: {BLUPR03MB5185823804C5C8ADCDE12FF93EB0-at-BLUPR03MB518.namprd03.prod.outlook.com} 11, 50 -- x-exchange-antispam-report-test: UriScan:(220697853802784)(47284530071512); 11, 50 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:(6040450)(601004)(2401047)(5005006)(8121501046)(93006095)(93001095)(10201501046)(3002001)(6041248)(20161123564025)(20161123562025)(20161123555025)(20161123560025)(20161123558100)(201703131423075)(201702281528075)(201703061421075)(201703061406153)(6072148);SRVR:BLUPR03MB518;BCL:0;PCL:0;RULEID:;SRVR:BLUPR03MB518; 11, 50 -- x-forefront-prvs: 02981BE340 11, 50 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10019020)(6009001)(39410400002)(39400400002)(39450400003)(252514010)(6916009)(6246003)(9686003)(7736002)(478600001)(966004)(50986999)(122556002)(25786009)(99286003)(55016002)(54356999)(2351001)(5640700003)(6306002)(6436002)(6506006)(38730400002)(77096006)(110136004)(5660300001)(2900100001)(189998001)(305945005)(86582002)(53936002)(7696004)(74316002)(2906002)(8936002)(6116002)(2501003)(3660700001)(3280700002)(8676002)(102836003)(81166006)(3846002)(86362001)(66066001)(229853002)(33656002);DIR:OUT;SFP:1102;SCL:1;SRVR:BLUPR03MB518;H:BLUPR03MB520.namprd03.prod.outlook.com;FPR:;SPF:None;MLV:sfv;LANG:en; 11, 50 -- spamdiagnosticoutput: 1:99 11, 50 -- spamdiagnosticmetadata: NSPM 11, 50 -- Content-Type: text/plain; charset="iso-8859-1" 11, 50 -- MIME-Version: 1.0 11, 50 -- X-OriginatorOrg: mpfi.org 11, 50 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 05 May 2017 13:52:37.6114 11, 50 -- (UTC) 11, 50 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted 11, 50 -- X-MS-Exchange-CrossTenant-id: 947b4551-7db4-4636-a5fd-1bdcad603ed0 11, 50 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: BLUPR03MB518 11, 50 -- Content-Transfer-Encoding: 8bit 11, 50 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v45DfQeF006884 ==============================End of - Headers==============================
From wandaver2-at-gmail.com Sat May 6 17:46:48 2017 Return-Path: {wandaver2-at-gmail.com} Received: from gmail.com ([203.255.31.195]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v46MkfrP024266 for {microscopylistserverarchive6-at-microscopy.com} ; Sat, 6 May 2017 17:46:47 -0500 Message-ID: {D5D9E4A8.78AFD7A1-at-gmail.com}
X-from: mikegf-at-hmamail.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mikegf-at-hmamail.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: mikegf-at-hmamail.com Name: Mike
Title-Subject: [Filtered] HBO 100 light leak
Message: I am searching on literature about the potential hazards of having light leaking out of an HBO 100 illuminator. There is plenty about how dangerous a mercury discharge can be if a lamp explodes. Can someone direct me or share with me information concerning the dangers of users being exposed to light leaking from a lamp housing?
Login Host: 78.22.73.101 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
Mike, We used HBO 100 lamps in out Reichert MeF3 metallograph for several decades. Reflected light out of the illuminator cooling vents or leaking light at the illuminator/microscope joint was never cautioned against; this was 30+ years ago, but I suppose the explanation from the factory trainer was that the internal surfaces were sufficiently poor optical and UV reflectors that once the light was reflected it wasn't a concern. Of course, the potential for permanent retina damage made direct viewing of the lamp an absolute prohibition - never ignite the bulb without the protective cover in place or look directly into the optical path. That's somewhat obvious to us, but the general public doesn't comprehend the extreme brightness of these lamps. Rick
Richard A. Ross Sr. Metallurgist, Materials Engineering Allison Transmission Inc.
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Sunday, May 07, 2017 10:24 AM To: Richard A. Ross
X-from: mikegf-at-hmamail.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mikegf-at-hmamail.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: mikegf-at-hmamail.com Name: Mike
Title-Subject: [Filtered] HBO 100 light leak
Message: I am searching on literature about the potential hazards of having light leaking out of an HBO 100 illuminator. There is plenty about how dangerous a mercury discharge can be if a lamp explodes. Can someone direct me or share with me information concerning the dangers of users being exposed to light leaking from a lamp housing?
Login Host: 78.22.73.101 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
Has anyone experienced problems with LaB6 cathodes in their TEM e.g. high dark current, beam instability? I have a vague recollection of historic issues mentioned on the List server.
The two LaB6 that we purchased in 2014 and recently installed in our CM10 FEI TEM are extremely problematic. The first one caused an array of issues notably high dark current, beam instability flashing/discharging. Much trouble shooting searching was done by lab staff and FEI service engineers, cleaning of anode, wehnelts and insulator, replenishing gun insulating oil to rule out other potential causes. We also tried the spare LaB6 cathode which demonstrated the same issues. Installing a tungsten as a last resort resulted in the EM quickly returning back to usable status with none of these issues.
I have used Lab6 from this manufacturer for years without any problems. Perhaps it is time to move on to another.
I would be interested to hear from others experiencing these or similar issues.
Levina
This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender.
Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities.
==============================Original Headers============================== 12, 36 -- From Levina.Dear-at-health.nsw.gov.au Mon May 8 18:07:52 2017 12, 36 -- Received: from smtp1.health.nsw.gov.au (smtp1.health.nsw.gov.au [203.5.104.28]) 12, 36 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v48N7pxi026249 12, 36 -- for {Microscopy-at-microscopy.com} ; Mon, 8 May 2017 18:07:52 -0500 12, 36 -- X-IronPort-Anti-Spam-Filtered: true 12, 36 -- X-IronPort-Anti-Spam-Result: A2BoCQAG/BBZ/xYjFgpcHAEBBAEBCgEBFwEBBAEBCgEBg3yBI7dRhiQChSgUAQEBAQEBAQEBAQGBBwuCMyCDAxk4ASoUQiYBBBsMwCCLKYZfhEWFARiDNoIxBYc4BYIPlC2jP4FFlD42gSsmCQIeHV+Gc4hUgQ0BAQE 12, 36 -- X-IPAS-Result: A2BoCQAG/BBZ/xYjFgpcHAEBBAEBCgEBFwEBBAEBCgEBg3yBI7dRhiQChSgUAQEBAQEBAQEBAQGBBwuCMyCDAxk4ASoUQiYBBBsMwCCLKYZfhEWFARiDNoIxBYc4BYIPlC2jP4FFlD42gSsmCQIeHV+Gc4hUgQ0BAQE 12, 36 -- X-IronPort-AV: E=Sophos;i="5.38,311,1491228000"; 12, 36 -- d="scan'208";a="143236240" 12, 36 -- Received: from cmdcipr-esa002.nswhealth.net ([10.22.35.22]) 12, 36 -- by smtp1.health.nsw.gov.au with ESMTP; 09 May 2017 09:18:58 +1000 12, 36 -- X-IronPort-Anti-Spam-Filtered: true 12, 36 -- X-IronPort-Anti-Spam-Result: A2BqCQBb/BBZ/4N0FApcHAEBBAEBCgEBhCeBI7dRhiQChSgUAQIBAQEBAQEBgQgLhVYZOAEqFEImAQQbDMAbiymGX4RFhQEYgzaCMQWHOAWCD5Qtoz+BRZQ+NoErJgkCHh1fhnOIVIENAQEB 12, 36 -- X-IronPort-AV: E=Sophos;i="5.38,311,1491228000"; 12, 36 -- d="scan'208";a="111000404" 12, 36 -- Received: from lvdchub-mex001.nswhealth.net ([10.20.116.131]) 12, 36 -- by cmdcipr-esa002-prv.nswhealth.net with ESMTP; 09 May 2017 09:18:41 +1000 12, 36 -- Received: from LVDCMBX-MEX001.nswhealth.net ([fe80::bd15:7a8:52fd:4a2c]) by 12, 36 -- LVDCHUB-MEX001.nswhealth.net ([fe80::d970:d96:15a:9fb4%17]) with mapi id 12, 36 -- 14.03.0224.002; Tue, 9 May 2017 09:18:40 +1000 12, 36 -- From: Levina Dear {Levina.Dear-at-health.nsw.gov.au} 12, 36 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 12, 36 -- Subject: LaB6 filaments/ cathode problems 12, 36 -- Thread-Topic: LaB6 filaments/ cathode problems 12, 36 -- Thread-Index: AdLIUPsBusSrvK/JQfyNtnbE+5bpow== 12, 36 -- Date: Mon, 8 May 2017 23:18:40 +0000 12, 36 -- Message-ID: {388DB4F50D9F574793BCE3F4C1923EEE6791C0F0-at-LVDCMBX-MEX001.nswhealth.net} 12, 36 -- Accept-Language: en-AU, en-US 12, 36 -- Content-Language: en-US 12, 36 -- X-MS-Has-Attach: 12, 36 -- X-MS-TNEF-Correlator: 12, 36 -- x-originating-ip: [10.20.59.160] 12, 36 -- Content-Type: text/plain; charset="us-ascii" 12, 36 -- MIME-Version: 1.0 12, 36 -- Content-Transfer-Encoding: 8bit 12, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v48N7pxi026249 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both pchapman-at-latech.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] postion - for Microsocopy, Metrology, Microfabrication - Louisiana Tech University
Message: Institute for Micromanufacturing is looking for Research Assistant Professor (non-tenure track)for Microscopy, Metrology, and Microfabrication.
Applicants must have a Ph.D. degree or equivalent in chemistry, physics, material science, engineering, or related technical field.
For details and application instructions please go to the Louisiana Tech University employment web site or directly to the posting at: http://finance.latech.edu/hr/vacan2561.php
Login Host: 138.47.56.81 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
LaB6 units are extremely dependant on vacuum level for their stability. Looking at the tests you have run I would be interested in the vacuum in the gun, not as given by the microscope but by monitoring as near as possible to the gun area, perhaps moving a microscope gauge nearer to that area if possible?
Steve
Steve Chapman FRMS Protrain for Consultancy and Training in Electron Microscopy +44 (0)7711 606967 web www.emcourses.com
-----Original Message----- X-from: Levina.Dear-at-health.nsw.gov.au [mailto:Levina.Dear-at-health.nsw.gov.au] Sent: 09 May 2017 00:09 To: protrain-at-emcourses.com
Hello microscopy community,
Regarding LaB6 filaments/ cathodes-
Has anyone experienced problems with LaB6 cathodes in their TEM e.g. high dark current, beam instability? I have a vague recollection of historic issues mentioned on the List server.
The two LaB6 that we purchased in 2014 and recently installed in our CM10 FEI TEM are extremely problematic. The first one caused an array of issues notably high dark current, beam instability flashing/discharging. Much trouble shooting searching was done by lab staff and FEI service engineers, cleaning of anode, wehnelts and insulator, replenishing gun insulating oil to rule out other potential causes. We also tried the spare LaB6 cathode which demonstrated the same issues. Installing a tungsten as a last resort resulted in the EM quickly returning back to usable status with none of these issues.
I have used Lab6 from this manufacturer for years without any problems. Perhaps it is time to move on to another.
I would be interested to hear from others experiencing these or similar issues.
Levina
This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender.
Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities.
==============================Original Headers============================== 12, 36 -- From Levina.Dear-at-health.nsw.gov.au Mon May 8 18:07:52 2017 12, 36 -- Received: from smtp1.health.nsw.gov.au (smtp1.health.nsw.gov.au [203.5.104.28]) 12, 36 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v48N7pxi026249 12, 36 -- for {Microscopy-at-microscopy.com} ; Mon, 8 May 2017 18:07:52 -0500 12, 36 -- X-IronPort-Anti-Spam-Filtered: true 12, 36 -- X-IronPort-Anti-Spam-Result: A2BoCQAG/BBZ/xYjFgpcHAEBBAEBCgEBFwEBBAEBCgEBg3yBI7dRhiQChSgUAQEBAQEBAQEBAQGB BwuCMyCDAxk4ASoUQiYBBBsMwCCLKYZfhEWFARiDNoIxBYc4BYIPlC2jP4FFlD42gSsmCQIeHV+G c4hUgQ0BAQE 12, 36 -- X-IPAS-Result: A2BoCQAG/BBZ/xYjFgpcHAEBBAEBCgEBFwEBBAEBCgEBg3yBI7dRhiQChSgUAQEBAQEBAQEBAQGB BwuCMyCDAxk4ASoUQiYBBBsMwCCLKYZfhEWFARiDNoIxBYc4BYIPlC2jP4FFlD42gSsmCQIeHV+G c4hUgQ0BAQE 12, 36 -- X-IronPort-AV: E=Sophos;i="5.38,311,1491228000"; 12, 36 -- d="scan'208";a="143236240" 12, 36 -- Received: from cmdcipr-esa002.nswhealth.net ([10.22.35.22]) 12, 36 -- by smtp1.health.nsw.gov.au with ESMTP; 09 May 2017 09:18:58 +1000 12, 36 -- X-IronPort-Anti-Spam-Filtered: true 12, 36 -- X-IronPort-Anti-Spam-Result: A2BqCQBb/BBZ/4N0FApcHAEBBAEBCgEBhCeBI7dRhiQChSgUAQIBAQEBAQEBgQgLhVYZOAEqFEIm AQQbDMAbiymGX4RFhQEYgzaCMQWHOAWCD5Qtoz+BRZQ+NoErJgkCHh1fhnOIVIENAQEB 12, 36 -- X-IronPort-AV: E=Sophos;i="5.38,311,1491228000"; 12, 36 -- d="scan'208";a="111000404" 12, 36 -- Received: from lvdchub-mex001.nswhealth.net ([10.20.116.131]) 12, 36 -- by cmdcipr-esa002-prv.nswhealth.net with ESMTP; 09 May 2017 09:18:41 +1000 12, 36 -- Received: from LVDCMBX-MEX001.nswhealth.net ([fe80::bd15:7a8:52fd:4a2c]) by 12, 36 -- LVDCHUB-MEX001.nswhealth.net ([fe80::d970:d96:15a:9fb4%17]) with mapi id 12, 36 -- 14.03.0224.002; Tue, 9 May 2017 09:18:40 +1000 12, 36 -- From: Levina Dear {Levina.Dear-at-health.nsw.gov.au} 12, 36 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 12, 36 -- Subject: LaB6 filaments/ cathode problems 12, 36 -- Thread-Topic: LaB6 filaments/ cathode problems 12, 36 -- Thread-Index: AdLIUPsBusSrvK/JQfyNtnbE+5bpow== 12, 36 -- Date: Mon, 8 May 2017 23:18:40 +0000 12, 36 -- Message-ID: {388DB4F50D9F574793BCE3F4C1923EEE6791C0F0-at-LVDCMBX-MEX001.nswhealth.net} 12, 36 -- Accept-Language: en-AU, en-US 12, 36 -- Content-Language: en-US 12, 36 -- X-MS-Has-Attach: 12, 36 -- X-MS-TNEF-Correlator: 12, 36 -- x-originating-ip: [10.20.59.160] 12, 36 -- Content-Type: text/plain; charset="us-ascii" 12, 36 -- MIME-Version: 1.0 12, 36 -- Content-Transfer-Encoding: 8bit 12, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v48N7pxi026249 ==============================End of - Headers==============================
==============================Original Headers============================== 24, 22 -- From protrain-at-emcourses.com Tue May 9 04:12:01 2017 24, 22 -- Received: from mail.esentra.net (mail.esentra.net [185.17.175.100]) 24, 22 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v499C0hs002945 24, 22 -- for {microscopy-at-microscopy.com} ; Tue, 9 May 2017 04:12:01 -0500 24, 22 -- Received: from mail.clientmail.net (Not Verified[172.16.2.101]) by mail.esentra.net with Esentra Mail Gateway 24, 22 -- id {B59118a880000} ; Tue, 09 May 2017 10:23:20 +0100 24, 22 -- Received: from ([127.0.0.1]) with MailEnable ESMTP; Tue, 9 May 2017 10:23:19 +0100 24, 22 -- From: "Steve Chapman" {protrain-at-emcourses.com} 24, 22 -- To: {Levina.Dear-at-health.nsw.gov.au} 24, 22 -- Cc: {microscopy-at-microscopy.com} 24, 22 -- References: {201705082309.v48N95eI027313-at-microscopy.com} 24, 22 -- In-Reply-To: {201705082309.v48N95eI027313-at-microscopy.com} 24, 22 -- Subject: RE: [Microscopy] LaB6 filaments/ cathode problems 24, 22 -- Date: Tue, 9 May 2017 10:23:04 +0100 24, 22 -- Message-ID: {003101d2c8a5$dd4129c0$97c37d40$-at-com} 24, 22 -- MIME-Version: 1.0 24, 22 -- Content-Type: text/plain; 24, 22 -- charset="us-ascii" 24, 22 -- Content-Transfer-Encoding: 7bit 24, 22 -- X-Mailer: Microsoft Office Outlook 12.0 24, 22 -- Thread-Index: AdLIUayNYlHnWG0gRuWoj+U+5jPrkgAU85tQ 24, 22 -- Content-Language: en-gb ==============================End of - Headers==============================
We would like to draw your attention to a new software system called FOCUS, which we find quite handy to work together with automated cryo-EM data collection, as done for example with SerialEM.
FOCUS is a C++ / Qt front end, running own and third-party softwares in the background. It features a GUI that allows you to define and edit parameters and C-shell or Python scripts, which are then executed in queues. Users can add or edit their own scripts, to adapt FOCUS to specific workflows or tasks. FOCUS comes with a set of default scripts, ready to run. Installation of FOCUS requires also the installation of any third-party programs that you want FOCUS to utilise, such as IMOD, EMAN, FREALIGN, or MotionCor2, UNBLUR, Zorro, CTFFIND4, gCTF, gAutomatch, or others.
If you install FOCUS on a strong Linux machine on a computer adjacent to the automated SerialEM run, then FOCUS can for example be used to: - monitor the file system of the SerialEM run - fetch newly recorded movies from the SerialEM computer, together with the pixel-defect-list and gain reference files, - unpack the compressed movies (TIFF ZLW), gain correct, - Fourier crop 8k to 4k, or to any pixel size you want (optionally) - drift-correct (with ZORRO, MotionCor2, or Unblur) - computer 2D averages of the movie, and FFTs of all files - measure defocus (gCTF or CTFFIND4) - pick particles (gAutomatch) - present the last recorded image and its FFT (before and after drift correction), and the drift trajectory plot and CTF Thon ring plot on a website that you would have to setup (ours is at https://status.c-cina.unibas.ch. Ours is open and shows blurred images, but you can protect it with a password and then display the crisp images) - present the statistics of each recorded image on that web site (as plots over time) - organize all recorded movies in a large table (similar to an Excel sheet), which can be sorted by various parameters such as -- grey value (which images are too dark (contaminated, beam lost) or too white (empty hole?) -- defocus (which images are in focus or totally out of focus?) -- CTF resolution (for which images is CTF determination difficult?) -- iciness (which images have a too high ratio of crystalline ice, or show devitrified sample?) -- drift (which images have too long or too short or too jittery drift trajectories? which images have too high remaining interframe drift?) -- and various other parameters. This table can be sorted by any of such parameters, and images can be moved into a TRASH folder with one click. - remaining images can be exported in folder structures and STAR files ready for subsequent processing in, e.g., Relion.
FOCUS can also be used for tomography sessions, to: - recognize parameters from the file name (e.g., specimen number and tilt angle) and organize files accordingly - drift-correct each recorded movie at a certain tilt angle, while taking the current electron dose and the prior electron dose from earlier tilt angle recordings on the same specimen into account for electron-dose-dependent B-factor resolution filtering - compute one 2D image per tilt angle movie - re-organize the tilt angle images by tilt angle (-60,-59,…,0,…,59,60) when recorded in the Hagen scheme (0,1,-1,-2,2,3,-3,-4,4,5,…60,-60) - as above: monitor data collection progress on a web site, sort data, assist in manually pruning of data, - export data in MRC stacks with one frame per tilt angle. - write out metadata for the stacks into STAR files
FOCUS can also be used for 2D crystal sessions, where it can do the entire 2D crystal processing automatically (the full functionality of the 2dx software package is included in FOCUS). This allows direct 3D reconstructions during the 2D crystal data collection session.
FOCUS maintains a batch queue, with which is runs all processes above in parallel.
We have installed this on an Ubuntu PC with 2x12 cores (= 48 threads) 256 GB RAM (of which 96GB are used as a RAM disk. More would make life easier.) 70 TB HD (RAID5) 2 x GTX1080 GPU We also use a 40” 4k monitor, which is great to display the images full-screen in full quality. We have one such system per automated cryo-EM microscope, which is able to keep up with single particle data collection of 40-frame 8k movies at a rate of 90 movies per hour (Fourier cropping, drift correction wth MotionCor2 on 5x5 patches, CTFFIND4, gautomatch, etc.), and is thereby on par with data collection.
FOCUS is described in Biyani et al., J. Struct. Biol., 2017 (https://www.ncbi.nlm.nih.gov/pubmed/28344036), also available on bioRxiv at https://doi.org/10.1101/105452. The software is open-source and freely available in precompiled versions for Linux and OS X. A manual in wiki form is available, all at http://focus-em.org.
Nikhil Biyani, Ricardo D. Righetto, Robert McLeod, Daniel Caujolle-Bert, Daniel Castano-Diez, Kenneth N. Goldie, and Henning Stahlberg
Henning Stahlberg, PhD Prof. for Structural Biology, C-CINA, Biozentrum, University Basel Mattenstrasse 26 | D-BSSE | WRO-1058 | CH-4058 Basel | Switzerland http://c-cina.org | Tel. +41-61-387 32 62
Not having seen any replies so far, I will jump in.
I had an IXRF system for over ten years; indeed, we were one of the early customers. However, I cannot recall the specifics of their MDL numbers.
MDL should mean the actual lower detectable concentration given the parameters of the collection. Therefore, it should drop with additional counts because the relative noise in the background is being reduced. If it does not change over time, then it could be a defined parameter which would not be correct in my opinion.
You may also want to look into the practicalities of their system and its calculations. (Remember, my information is several years out of date.) An old version of their software fitted the background and subtracted it and then rectified the data. That is, all of the negative counts in the spectrum were set to zero. That resulted in a positive integral and result for virtually any element even when none was present. The reported amount diminished over time (approached zero) for those cases where the element was not present. It may have been that the reported level was less than the reported MDL. I should have compared the number to the MDL and ignored results less than MDL. (Many systems flag results which are less than statistically significant, but some do it more clearly than others.) However, I think it would have been better if they had let the result wander around zero (both positive and negative) and settled in closer and closer to zero with time. I have heard from various software people that negative results are incomprehensible for many users so they (the developers) take steps which end up biasing the results.
For the record, I reported this matter to IXRF on at least two occasions and it had not been resolved the last time I checked which was several years back. I was told it was going to be addressed. I certainly hope it has been fixed by now, but we may all be able to cite software issues that languish on the do-do list. Maybe one of their engineers is following the list and can clarify the matter.
Warren Straszheim
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Sunday, May 07, 2017 9:09 AM To: Straszheim, Warren E [BIOTC]
-------- Forwarded Message --------
X-from: andrew.kloiber-at-crl.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both andrew.kloiber-at-crl.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: andrew.kloiber-at-crl.com Name: Andrew Kloiber
Organization: Charles River Laboratories Title-Subject: [Filtered] Job Posting-- Electron Microscopy Technician II
Message: DEPARTMENT NAME: EM Laboratory POSITION TYPE: Full Time POSITION TITLE: Electron Microscopy Technician II
COMPANY SUMMARY: The Durham, NC Electron Microscopy Lab supports Charles River Laboratories, globally. The electron microscopy technician will join our EM staff of 7 and assist with sample preparation and ultrastructural examination of animal tissue and cell culture: processing, embedding, thin and ultrathin sectioning, negative staining, operating a Transmission Electron Microscope, and darkroom. Will also assist with inventory, maintenance of equipment, report writing, QC. Our work is performed under FDAs GLPs and accurate documentation is mandatory. Candidate should be comfortable working in a fast-paced environment. BS in biological science
Working knowledge of design, theory and operation of TEMs, including skill and a minimum of 1 full year experience in their operation. Proficient preparing solutions/reagents, sectioning on an ultramicrotome, staining slides and grids, developing film negatives, processing prints, and operating basic laboratory equipment, e.g., balance, pH meter.
Proficient with MS Word and Excel Excellent written and verbal communication skills. Able to interact daily with other members of the lab as well as the support staff, pathologists and management. Meticulous data recording required. Experience in ultrastructure of mammalian tissue and digital image acquisition a plus.
Login Host: 96.10.85.74 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both pchapman-at-latech.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] postion - for Microsocopy, Metrology, Microfabrication - Louisiana Tech University
Message: Institute for Micromanufacturing is looking for Research Assistant Professor (non-tenure track)for Microscopy, Metrology, and Microfabrication.
Applicants must have a Ph.D. degree or equivalent in chemistry, physics, material science, engineering, or related technical field.
For details and application instructions please go to the Louisiana Tech University employment web site or directly to the posting at: http://finance.latech.edu/hr/vacan2561.php
Login Host: 138.47.56.81 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both desertrw1-at-gmail.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: desertrw1-at-gmail.com Name: Russ Winstead
Organization: Self, Sandia Scientific, Inc
Title-Subject: [Filtered] Newby- recently acquired Jeol 5310 SEM needs schematics
Message: Hi- I am new to the microscopy list server, and I am excited about getting involved in this arena!
I recently purchased a surplus Jeol 5310 SEM that I am currently learning to use, I previously helped restore an older Jeol SEM about 15 years ago. The 5310 had an issue with the HT (High Tension enable) button not working after initial vacuum pumpdown. After tracing the button wires I found that somebody had spilled some coffee (appearance anyway) onto the circuit boards beneath the console. I unsoldered several components, cleaned the boards, and re-installed the components- HT has worked perfectly since then, I have been enjoying lots of close-ups of various watches, tiny filaments, etc. I am also planning to replace the original polaroid camera with a digital camera system, for now I simply take pictures of the main CRT display. Has anybody done this?
I am fearful that I will need the electrical schematics someday as this microscope becomes older. I do not have any of the original documentation, it was somehow lost before I purchased it! I am hoping that somebody PLEASE PLEASE can make a copy of their operation manuals and schematics for the Jeol 5310?? I would be happy to compensate or purchase... Any documentation that is associated with the Jeol 5310 SEM would be appreciated!
I also would like to see if anybody has a sputter coater or carbon coater that needs repair or can be purchased for a reasonable price- I do not have either right now and I think bugs and other subjects are in my future!
I also have about 20 extra "K type" filaments (some used, some new) for the Jeol, maybe some type of trade or if anybody needs a few...
Comments, suggestions, and ideas are welcome- thanks in advance!
Russ
Login Host: 67.164.129.230 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both areplogle-at-pugetsound.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: areplogle-at-pugetsound.edu
Name: Amy Replogle
Organization: University of Puget Sound
Title-Subject: [Filtered] TEM Repair Recommendations
Message: Hi everyone,
We have a Zeiss CEM 902 Transmission Electron Microscope that is currently displaying the following error code:
3-1: Output voltage of high-voltage measuring tube below 0.1V (broken measuring line, defective measuring instrument, or contaminated measuring tube does not ignite). Zeiss no longer supports this instrument so we are looking for recommendations for maintenance and repair. Any suggestions? Thanks, Amy
--------------------------------------------------- Amy Replogle '05 Biology Department Adjunct Assistant Professor Science Core Facility Technician
Login Host: 207.207.127.230 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
when does the error code light up? only after quite a while when the EM was running?
I heard of a problem that in rare cases the vacuum becomes too good ( e.g. better than 10 minus 7) and thus the voltage exceeds the minimal value which is necessary. The people helped themselves via a needle valve continuously admitting a smallest amount of air.
good luck,
Peter ( happy user of Zeiss EM109 TEM)
++++++++++++++++++++++++++++++++++++++++++++++++
Am 13.05.2017 um 00:26 schrieb microscopy.listserver-at-gmail.com: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: areplogle-at-pugetsound.edu } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } ---------------------------------------------------------------------------w } Remember this posting is most likely not from a Subscriber, so when replying please copy both } areplogle-at-pugetsound.edu as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: areplogle-at-pugetsound.edu } } Name: Amy Replogle } } Organization: University of Puget Sound } } Title-Subject: [Filtered] TEM Repair Recommendations } } Message: Hi everyone, } } We have a Zeiss CEM 902 Transmission Electron Microscope that is currently displaying the following } error code: } } 3-1: Output voltage of high-voltage measuring tube below 0.1V (broken measuring line, defective } measuring instrument, or contaminated measuring tube does not ignite). } Zeiss no longer supports this instrument so we are looking for recommendations for maintenance and } repair. Any suggestions? } Thanks, } Amy } } --------------------------------------------------- } Amy Replogle '05 } Biology Department } Adjunct Assistant Professor } Science Core Facility Technician } } } Login Host: 207.207.127.230 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } }
==============================Original Headers============================== 9, 25 -- From peter.heimann-at-uni-bielefeld.de Sat May 13 06:19:06 2017 9, 25 -- Received: from unibi-smtp-a.hrz.uni-bielefeld.de (unibi-smtp-a.hrz.uni-bielefeld.de [129.70.208.12]) 9, 25 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v4DBIFtm005058 9, 25 -- for {Microscopy-at-microscopy.com} ; Sat, 13 May 2017 06:19:05 -0500 9, 25 -- MIME-version: 1.0 9, 25 -- Content-transfer-encoding: 7BIT 9, 25 -- Content-type: text/plain; CHARSET=US-ASCII; format=flowed 9, 25 -- Received: from [192.168.178.21] ([79.255.239.47]) 9, 25 -- by unibi-smtp-a.hrz.uni-bielefeld.de 9, 25 -- (Oracle Communications Messaging Server 7.0.5.37.0 64bit (built Jan 25 2016)) 9, 25 -- with ESMTPPA id {0OPW00GVK2LP7A70-at-unibi-smtp-a.hrz.uni-bielefeld.de} for 9, 25 -- Microscopy-at-microscopy.com; Sat, 13 May 2017 13:29:54 +0200 (CEST) 9, 25 -- X-Connecting-IP: [79.255.239.47] 9, 25 -- X-PMX-Version: 6.3.1.2588712, Antispam-Engine: 2.7.2.2107409, 9, 25 -- Antispam-Data: 2017.5.13.112415, pmx12 9, 25 -- X-EnvFrom: peter.heimann-at-uni-bielefeld.de 9, 25 -- Subject: Re: Zeiss CEM 902 TEM Repair Recommendations 9, 25 -- To: Microscopy-at-microscopy.com 9, 25 -- References: {201705122226.v4CMQ4iY017065-at-microscopy.com} 9, 25 -- From: Peter Heimann {peter.heimann-at-uni-bielefeld.de} 9, 25 -- Message-id: {828d13a7-4e2a-3070-a58d-f7998cb2e98f-at-uni-bielefeld.de} 9, 25 -- Date: Sat, 13 May 2017 13:29:49 +0200 9, 25 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:45.0) Gecko/20100101 9, 25 -- Thunderbird/45.8.0 9, 25 -- In-reply-to: {201705122226.v4CMQ4iY017065-at-microscopy.com} ==============================End of - Headers==============================
first it would be useful to know if the error comes from a Pirani or a Penning tube. Can you be more specific? Can you relate the error code with the location / kind of the measuring tube?
If Penning, I would first try to clean it. If the Penning is too contaminated, it looks like the vacuum is ultra-good ;-)
Procedure would be to set the part of the vacuum system the tube is fixed at on air, then switch off the mic completely, since there might be HIGH voltage on the tube.
You can send me an image of the measuring tube(s) and I can check if I have a working one to send over.
Best,
Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Am 13.05.17 um 02:50 schrieb microscopy.listserver-at-gmail.com: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: areplogle-at-pugetsound.edu } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } areplogle-at-pugetsound.edu as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: areplogle-at-pugetsound.edu } } Name: Amy Replogle } } Organization: University of Puget Sound } } Title-Subject: [Filtered] TEM Repair Recommendations } } Message: Hi everyone, } } We have a Zeiss CEM 902 Transmission Electron Microscope that is currently displaying the following } error code: } } 3-1: Output voltage of high-voltage measuring tube below 0.1V (broken measuring line, defective } measuring instrument, or contaminated measuring tube does not ignite). } Zeiss no longer supports this instrument so we are looking for recommendations for maintenance and } repair. Any suggestions? } Thanks, } Amy } } --------------------------------------------------- } Amy Replogle '05 } Biology Department } Adjunct Assistant Professor } Science Core Facility Technician } } } Login Host: 207.207.127.230 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } }
==============================Original Headers============================== 12, 28 -- From diller-at-stefan-diller.com Sat May 13 13:58:57 2017 12, 28 -- Received: from mailout013.rox.net (mailout013.rox.net [212.63.85.213]) 12, 28 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v4DIwrNV011257 12, 28 -- for {microscopy-at-microscopy.com} ; Sat, 13 May 2017 13:58:57 -0500 12, 28 -- Received: from localhost ([127.0.0.1]) 12, 28 -- by mailout01.rox.net with esmtp (Exim 4.80) 12, 28 -- (envelope-from {diller-at-stefan-diller.com} ) 12, 28 -- id 1d9cQu-00028B-0D; Sat, 13 May 2017 21:10:28 +0200 12, 28 -- Received: from p5dcb482e.dip0.t-ipconnect.de ([93.203.72.46] helo=mac-pro.local) 12, 28 -- by mailout01.rox.net with esmtpsa (TLSv1.2:DHE-RSA-AES128-SHA:128) 12, 28 -- (Exim 4.80) 12, 28 -- (envelope-from {diller-at-stefan-diller.com} ) 12, 28 -- id 1d9cQt-000285-SO; Sat, 13 May 2017 21:10:27 +0200 12, 28 -- Subject: Re: [Microscopy] viaWWW:Zeiss CEM 902 TEM Repair Recommendations 12, 28 -- To: "Microscopy-at-microscopy.com" {microscopy-at-microscopy.com} , 12, 28 -- areplogle-at-pugetsound.edu 12, 28 -- References: {201705130050.v4D0ohPH011416-at-microscopy.com} 12, 28 -- From: stefan diller {diller-at-stefan-diller.com} 12, 28 -- Message-ID: {4b3cbae8-7835-e687-f69d-741ec6bcbbf6-at-stefan-diller.com} 12, 28 -- Date: Sat, 13 May 2017 21:10:25 +0200 12, 28 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:45.0) 12, 28 -- Gecko/20100101 Thunderbird/45.8.0 12, 28 -- MIME-Version: 1.0 12, 28 -- In-Reply-To: {201705130050.v4D0ohPH011416-at-microscopy.com} 12, 28 -- Content-Type: text/plain; charset=windows-1252; format=flowed 12, 28 -- Content-Transfer-Encoding: 7bit 12, 28 -- X-Envelope-From: {diller-at-stefan-diller.com} 12, 28 -- X-Scanned-By: rockenstein AG ==============================End of - Headers==============================
It is with a heavy heart that we have to announce the passing of an advisory board member, and pioneer in SPM analysis, Dr. Scott W. Maclaren. He will be sorely missed as a fellow scientist and a close friend.
A picture of Scott, one of his popular SPM images, and the following article can be found here: https://www.linkedin.com/pulse/popular-inspiring-university-illinois-scientist-pioneer-allen-hall
One of the most popular and beloved scientists in the Midwest, who trained more than a thousand researchers and inspired creativity in thousands more, has died. Dr. Scott Maclaren, of the University of Illinois at Urbana–Champaign (UIUC), succumbed to complications related to diabetes on Tuesday, May 9th. He was 57.
Maclaren served as Senior Staff Scientist of UIUC’s Frederick Seitz Material Research Laboratory. The son of a General Electric executive, born in Syracuse, New York and raised in Chagrin Falls, Ohio, Maclaren was a childhood prodigy who showed an early aptitude in science and began taking university courses while still in high school. He attended the prestigious Massachusetts Institute of Technology (MIT) where he mastered several disciplines; taking on a double major in Physics and Planetary Science. While at MIT, Maclaren also taught physics to underclassmen and earned a reputation for his inspiring instruction style. He graduated in 1982 after writing a thesis on laser beam weaponry that several of his teachers were concerned might have to be classified.
Shortly after leaving MIT, Maclaren moved to UIUC where he remained for the rest of his life. Although he was considered an expert in many scientific disciplines, the field Maclaren was most famous in was Scanning Probe Microscopy, or SPM. Basically, a form of imaging of materials at the atomic level and used extensively in the emerging field of nanotechnology.
Maclaren’s knowledge and experience commanded global respect and his expertise was much sought after by many foreign institutions. Maclaren also worked as an advisor to several major companies in the United States and aboard. Among them were US-based Asylum Research and BudgetSensors of Bulgaria. Maclaren was also an advisor and mentor to Illinois-based Prairie Nanotechnology. Aside from giving lectures and tutorials on SPM at many national workshops and conferences, Maclaren was also involved in several outreach programs promoting nanotechnology in science education.
Even though Maclaren was plagued by numerous health issues in the latter part of his life, he still had a work ethic those around him sometimes found intimidating. Every weekend, Maclaren could be found in MRL’s Atomic Force Microscopy lab investigating any material he found interesting and juggling multiple projects.
After being checked into Presence Convent Medical Center in Urbana on May 3rd for what seemed to be a minor skin condition, his health quickly deteriorated. He died shortly thereafter.
Funeral arrangements are currently being finalized and will be private. A traditional memorial service unique to UIUC will be held later this year. In lieu of flowers, the family requests that donations be made in his memory to the Orpheum Children’s Science Museum (orpheumkids.net) or the Vasculitis Foundation (vasculitisfoundation.org).
With my sincere condolences to all of Scott's friends and colleagues, he will be sorely missed. -Allen J. Hall http://www.prairienanotech.com/
==============================Original Headers============================== 11, 48 -- From ajhall-at-prairienanotech.com Sat May 13 22:59:46 2017 11, 48 -- Received: from mail-it0-f46.google.com (mail-it0-f46.google.com [209.85.214.46]) 11, 48 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v4E3xUxK018699 11, 48 -- for {Microscopy-at-microscopy.com} ; Sat, 13 May 2017 22:59:45 -0500 11, 48 -- Received: by mail-it0-f46.google.com with SMTP id w68so7092495itc.0 11, 48 -- for {Microscopy-at-microscopy.com} ; Sat, 13 May 2017 21:11:16 -0700 (PDT) 11, 48 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 11, 48 -- d=prairienanotech-com.20150623.gappssmtp.com; s=20150623; 11, 48 -- h=message-id:date:from:user-agent:mime-version:to:subject 11, 48 -- :content-transfer-encoding; 11, 48 -- bh=u5KP3tOMSWGCXeZbFK6X08KKVAGsjFbyG05giibWVG4=; 11, 48 -- b=HfiY88HhC1xGBrj+H4Rqa4QswPaOdorFelXvXU5Fy7UjvkNA4vZr/OvxokaLVOt4HV 11, 48 -- vM36WTffTxrsZhYDFFM43tmZioWyN3vOoNd3kqPhubihx6oS3fxRnVg9sc75h8GTSBkO 11, 48 -- /Rq7QYYTXmgI5uDWizfA5i8Kn5KeilpV1O19BLp4CJwTcaWOXsUnPATYj92qGdkGNwGO 11, 48 -- /Iyf5dfOYXfMcivgFmI3nRmIrQtAMQtuXS2/ru9N9vQvlYVqgm7kxx10wZJ/ZPL4+6rE 11, 48 -- 8STWyr4MwLp6YbxwhylWSphCgXb7ryc6xuB9UQvY9y+3IwE4Zn+vUdu59mHMYsI6HNWI 11, 48 -- 0mBw== 11, 48 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 11, 48 -- d=1e100.net; s=20161025; 11, 48 -- h=x-gm-message-state:message-id:date:from:user-agent:mime-version:to 11, 48 -- :subject:content-transfer-encoding; 11, 48 -- bh=u5KP3tOMSWGCXeZbFK6X08KKVAGsjFbyG05giibWVG4=; 11, 48 -- b=B8tuc2/tVUWb+69n4B7+yAWfvmwpxGyj1PNAnMuLDJWfdRvVvTxe7m8ry/JQSXhj4c 11, 48 -- sC74hz/jOrvufUqg905aaBcVAo28jdGTDDVRSEu/CnL5Bv2gr7JeTeWkgkLHLd6qPeII 11, 48 -- 3DG2klvlhjblWaD6hMRRnEQnxYaGk9fRHNCpTHDw35igSh0tKD75mQepVSy0QEqmOKVo 11, 48 -- UuuHX6pC6s8DXHSKbe7dn3K+LfyKwwCti9o58LNRKLV7G7dHFILDkeseRO6ZtXtiCpkD 11, 48 -- /DFfUMl8mtMQYdg/y+F3x4yJhBm5HP340/ZRagbqYwTs0bG+d149UOvfkISykHkiZtKq 11, 48 -- QDVw== 11, 48 -- X-Gm-Message-State: AODbwcBfydcr2u9ovbLVy1Akyl75BKziUhLBJInIuDXEK7oJVwh6c8zz 11, 48 -- JxpDii8zQuSb823lg9pcsQ== 11, 48 -- X-Received: by 10.36.121.22 with SMTP id z22mr3912969itc.59.1494735071450; 11, 48 -- Sat, 13 May 2017 21:11:11 -0700 (PDT) 11, 48 -- Received: from AllenH.local (mobile-130-126-255-81.near.illinois.edu. [130.126.255.81]) 11, 48 -- by smtp.googlemail.com with ESMTPSA id y7sm2944257itc.27.2017.05.13.21.11.10 11, 48 -- for {Microscopy-at-microscopy.com} 11, 48 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 11, 48 -- Sat, 13 May 2017 21:11:10 -0700 (PDT) 11, 48 -- Message-ID: {5917D8DC.2060102-at-prairienanotech.com} 11, 48 -- Date: Sat, 13 May 2017 23:11:08 -0500 11, 48 -- From: Allen Hall {ajhall-at-prairienanotech.com} 11, 48 -- User-Agent: Postbox 5.0.13 (Macintosh/20170501) 11, 48 -- MIME-Version: 1.0 11, 48 -- To: Microscopy-at-microscopy.com 11, 48 -- Subject: Popular, Inspiring University of Illinois Scientist and Nanotechnology 11, 48 -- Pioneer Scott Maclaren Dies at 57 11, 48 -- X-Enigmail-Version: 1.2.3 11, 48 -- Content-Type: text/plain; charset=UTF-8 11, 48 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
My original message; Has anyone experienced problems with LaB6 cathodes in their TEM e.g. high dark current, beam instability? I have a vague recollection of historic issues mentioned on the List server.
The two LaB6 that we purchased in 2014 and recently installed in our CM10 FEI TEM are extremely problematic. The first one caused an array of issues notably high dark current, beam instability flashing/discharging. Much trouble shooting searching was done by lab staff and FEI service engineers, cleaning of anode, wehnelts and insulator, replenishing gun insulating oil to rule out other potential causes. We also tried the spare LaB6 cathode which demonstrated the same issues. Installing a tungsten as a last resort resulted in the EM quickly returning back to usable status with none of these issues.
I have used Lab6 from this manufacturer for years without any problems. Perhaps it is time to move on to another.
I would be interested to hear from others experiencing these or similar issues. ---------------------------------------------------------------------------------------------------------------------------------
Summary of some of the responses; -cleaned the legs of the filament (emery paper then washed with acetone) resolved the problems. -that LaB6 filaments have a limited shelf life. (I have not seen a use by date on any that we have purchased) -extremely dependant on vacuum level for their stability. -LaB6 filaments, from crystals cleaving to bases coming loose - and it seems from multiple suppliers.
Result; A new LaB6 was purchased from the same manufacturer, it was installed and is working beautifully, so good to see such a bright green glow again - problem fixed!
I am waiting to hear back from the manufacturer regarding the two LaB6 that were returned.
Kind regards Levina
This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender.
Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities.
==============================Original Headers============================== 15, 37 -- From Levina.Dear-at-health.nsw.gov.au Mon May 15 01:34:21 2017 15, 37 -- Received: from smtp2.health.nsw.gov.au (smtp2.health.nsw.gov.au [203.5.105.29]) 15, 37 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v4F6YJTl022901 15, 37 -- for {Microscopy-at-microscopy.com} ; Mon, 15 May 2017 01:34:20 -0500 15, 37 -- X-IronPort-Anti-Spam-Filtered: true 15, 37 -- X-IronPort-Anti-Spam-Result: A2AJGgBuThlZ/xY7FApcHQEFAQsBGQYMg3yBI7ddhiQChWETAQEBAQEBAQEBAQGBEkIOgWMigx04ASpWJgEEGxG5Q4p4hl+ERYRzDhiDP4IxBYc6BYIFCocXjSWjQYFFlEM3gSomCQIeHV+GdoUrgW2BMIENAQEB 15, 37 -- X-IPAS-Result: A2AJGgBuThlZ/xY7FApcHQEFAQsBGQYMg3yBI7ddhiQChWETAQEBAQEBAQEBAQGBEkIOgWMigx04ASpWJgEEGxG5Q4p4hl+ERYRzDhiDP4IxBYc6BYIFCocXjSWjQYFFlEM3gSomCQIeHV+GdoUrgW2BMIENAQEB 15, 37 -- X-IronPort-AV: E=Sophos;i="5.38,343,1491228000"; 15, 37 -- d="scan'208";a="139657197" 15, 37 -- Received: from lvdcipr-esa002.nswhealth.net ([10.20.59.22]) 15, 37 -- by smtp2.health.nsw.gov.au with ESMTP; 15 May 2017 16:45:56 +1000 15, 37 -- X-IronPort-Anti-Spam-Filtered: true 15, 37 -- X-IronPort-Anti-Spam-Result: A2AEGgCBTRlZ/4x0FApcHQEFAQsBhCeBI7ddhiQChWETAQIBAQEBAQEBgRNCDoUiOAEqViYBBBsRuUKKeIZfhEWEcw4Ygz+CMQWHOgWCBQqHF40lo0GBRZRDN4EqJgkCHh1fhnaFK4FtgTCBDQEBAQ 15, 37 -- X-IronPort-AV: E=Sophos;i="5.38,343,1491228000"; 15, 37 -- d="scan'208";a="42909328" 15, 37 -- Received: from lvdchub-mex002.nswhealth.net ([10.20.116.140]) 15, 37 -- by lvdcipr-esa002-prv.nswhealth.net with ESMTP; 15 May 2017 16:45:56 +1000 15, 37 -- Received: from LVDCMBX-MEX001.nswhealth.net ([fe80::bd15:7a8:52fd:4a2c]) by 15, 37 -- LVDCHUB-MEX002.nswhealth.net ([fe80::b14f:cc31:e072:908c%17]) with mapi id 15, 37 -- 14.03.0224.002; Mon, 15 May 2017 16:45:56 +1000 15, 37 -- From: Levina Dear {Levina.Dear-at-health.nsw.gov.au} 15, 37 -- To: "Listserver (plain text only) (Microscopy-at-microscopy.com)" 15, 37 -- {Microscopy-at-microscopy.com} 15, 37 -- Subject: LaB6 filament issues 15, 37 -- Thread-Topic: LaB6 filament issues 15, 37 -- Thread-Index: AdLNRRHRmgJPRQL4QR+Wd0sYigzW+Q== 15, 37 -- Date: Mon, 15 May 2017 06:45:55 +0000 15, 37 -- Message-ID: {388DB4F50D9F574793BCE3F4C1923EEE6791C9AF-at-LVDCMBX-MEX001.nswhealth.net} 15, 37 -- Accept-Language: en-AU, en-US 15, 37 -- Content-Language: en-US 15, 37 -- X-MS-Has-Attach: 15, 37 -- X-MS-TNEF-Correlator: 15, 37 -- x-originating-ip: [10.20.59.160] 15, 37 -- Content-Type: text/plain; charset="iso-8859-1" 15, 37 -- MIME-Version: 1.0 15, 37 -- Content-Transfer-Encoding: 8bit 15, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v4F6YJTl022901 ==============================End of - Headers==============================
From wandaver2-at-gmail.com Mon May 15 11:19:32 2017 Return-Path: {wandaver2-at-gmail.com} Received: from gmail.com ([211.233.57.227]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v4FGJQj4004667; Mon, 15 May 2017 11:19:29 -0500 Message-ID: {13622DA8.E52C697C-at-gmail.com}
X-from: ling.xie-at-angstrom.uu.se
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ling.xie-at-angstrom.uu.se as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: ling.xie-at-angstrom.uu.se Name: Ling Xie
Organization: Uppsala University
Title-Subject: [Filtered] 3rd International workshop on TEM spectroscopy in materials science Message: Dear Microscopists,
We organize the 3rd international workshop on TEM spectroscopy in materials science in Uppsala (19th-22th June), Sweden. We have invited a number of colleagues with a long and excellent background from the field of TEM spectroscopy and have made strong efforts to keep the registration fees very low (150 euros for Students, 200 Euros for Researchers). We believe that this could make the workshop interesting for some of your co-workers or students. Uppsala is the closest city to Stockholm airport (18min) and the airport is easy to reach for national and international flights. Early bird registration deadline is 19th of May, this friday! Please, would you be so kind and forward the attached flyer and hang it up in your corridor? We would be very pleased, if we could meet you and your group members on the workshop!
If you have any questions, please send an e-mail to: tem-workshop-at-angstrom.uu.se
Best regards, Organization committee
Login Host: 130.238.22.149 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both hasan.ali-at-angstrom.uu.se as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: hasan.ali-at-angstrom.uu.se Name: Hasan Ali
Organization: Uppsala University
Title-Subject: [Filtered] How to change the EELS acquisition area on CCD
Message: In electron energy loss spectroscopy, only a small central part of the CCD, vertical to the energy dispersion axis, is used to acquire the spectrum. If I acquire the camera image of EELS, I get such kind of spectrum on the CCD. For my application, I want to extend the vertical range of EELS on the CCD. I changed the camera coordinates in the "EELS acquire" setup, but it doesn't change the acquisition area of EELS on CCD. Can someone help me to know how can I change the area of the CCD used for EELS acquisition? I am usin a GIF2002. Thanks
Login Host: 185.113.96.230 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
This is a shock to me. I always enjoyed talking with Scott and visiting his lab. regards, Don ============================================= Don Chernoff, Ph.D., President Advanced Surface Microscopy, Inc.; 3250 N. Post Rd., Suite 120; Indianapolis IN 46226 USA E-Mail: donc-at-asmicro.com www.asmicro.com Voice: +1-317-895-5630 Toll free: +1-800-374-8557 (in USA & Canada) Fax: +1-317-895-5652 [business activities since 1990: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.] ============================================= ----- Original Message ----- From: ajhall-at-prairienanotech.com To: donc-at-asmicro.com Sent: Sunday, May 14, 2017 1:17 AM Subject: [a] [Microscopy] Popular, Inspiring University of Illinois Scientist and Nanotechnology
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html ----------------------------------------------------------------------------
It is with a heavy heart that we have to announce the passing of an advisory board member, and pioneer in SPM analysis, Dr. Scott W. Maclaren. He will be sorely missed as a fellow scientist and a close friend.
A picture of Scott, one of his popular SPM images, and the following article can be found here: https://www.linkedin.com/pulse/popular-inspiring-university-illinois-scientist-pioneer-allen-hall
One of the most popular and beloved scientists in the Midwest, who trained more than a thousand researchers and inspired creativity in thousands more, has died. Dr. Scott Maclaren, of the University of Illinois at UrbanabChampaign (UIUC), succumbed to complications related to diabetes on Tuesday, May 9th. He was 57.
Maclaren served as Senior Staff Scientist of UIUCbs Frederick Seitz Material Research Laboratory. The son of a General Electric executive, born in Syracuse, New York and raised in Chagrin Falls, Ohio, Maclaren was a childhood prodigy who showed an early aptitude in science and began taking university courses while still in high school. He attended the prestigious Massachusetts Institute of Technology (MIT) where he mastered several disciplines; taking on a double major in Physics and Planetary Science. While at MIT, Maclaren also taught physics to underclassmen and earned a reputation for his inspiring instruction style. He graduated in 1982 after writing a thesis on laser beam weaponry that several of his teachers were concerned might have to be classified.
Shortly after leaving MIT, Maclaren moved to UIUC where he remained for the rest of his life. Although he was considered an expert in many scientific disciplines, the field Maclaren was most famous in was Scanning Probe Microscopy, or SPM. Basically, a form of imaging of materials at the atomic level and used extensively in the emerging field of nanotechnology.
Maclarenbs knowledge and experience commanded global respect and his expertise was much sought after by many foreign institutions. Maclaren also worked as an advisor to several major companies in the United States and aboard. Among them were US-based Asylum Research and BudgetSensors of Bulgaria. Maclaren was also an advisor and mentor to Illinois-based Prairie Nanotechnology. Aside from giving lectures and tutorials on SPM at many national workshops and conferences, Maclaren was also involved in several outreach programs promoting nanotechnology in science education.
Even though Maclaren was plagued by numerous health issues in the latter part of his life, he still had a work ethic those around him sometimes found intimidating. Every weekend, Maclaren could be found in MRLbs Atomic Force Microscopy lab investigating any material he found interesting and juggling multiple projects.
After being checked into Presence Convent Medical Center in Urbana on May 3rd for what seemed to be a minor skin condition, his health quickly deteriorated. He died shortly thereafter.
Funeral arrangements are currently being finalized and will be private. A traditional memorial service unique to UIUC will be held later this year. In lieu of flowers, the family requests that donations be made in his memory to the Orpheum Childrenbs Science Museum (orpheumkids.net) or the Vasculitis Foundation (vasculitisfoundation.org).
With my sincere condolences to all of Scott's friends and colleagues, he will be sorely missed. -Allen J. Hall http://www.prairienanotech.com/
==============================Original Headers============================== 11, 48 -- From ajhall-at-prairienanotech.com Sat May 13 22:59:46 2017 11, 48 -- Received: from mail-it0-f46.google.com (mail-it0-f46.google.com [209.85.214.46]) 11, 48 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v4E3xUxK018699 11, 48 -- for {Microscopy-at-microscopy.com} ; Sat, 13 May 2017 22:59:45 -0500 11, 48 -- Received: by mail-it0-f46.google.com with SMTP id w68so7092495itc.0 11, 48 -- for {Microscopy-at-microscopy.com} ; Sat, 13 May 2017 21:11:16 -0700 (PDT) 11, 48 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 11, 48 -- d=prairienanotech-com.20150623.gappssmtp.com; s=20150623; 11, 48 -- h=message-id:date:from:user-agent:mime-version:to:subject 11, 48 -- :content-transfer-encoding; 11, 48 -- bh=u5KP3tOMSWGCXeZbFK6X08KKVAGsjFbyG05giibWVG4=; 11, 48 -- b=HfiY88HhC1xGBrj+H4Rqa4QswPaOdorFelXvXU5Fy7UjvkNA4vZr/OvxokaLVOt4HV 11, 48 -- vM36WTffTxrsZhYDFFM43tmZioWyN3vOoNd3kqPhubihx6oS3fxRnVg9sc75h8GTSBkO 11, 48 -- /Rq7QYYTXmgI5uDWizfA5i8Kn5KeilpV1O19BLp4CJwTcaWOXsUnPATYj92qGdkGNwGO 11, 48 -- /Iyf5dfOYXfMcivgFmI3nRmIrQtAMQtuXS2/ru9N9vQvlYVqgm7kxx10wZJ/ZPL4+6rE 11, 48 -- 8STWyr4MwLp6YbxwhylWSphCgXb7ryc6xuB9UQvY9y+3IwE4Zn+vUdu59mHMYsI6HNWI 11, 48 -- 0mBw== 11, 48 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 11, 48 -- d=1e100.net; s=20161025; 11, 48 -- h=x-gm-message-state:message-id:date:from:user-agent:mime-version:to 11, 48 -- :subject:content-transfer-encoding; 11, 48 -- bh=u5KP3tOMSWGCXeZbFK6X08KKVAGsjFbyG05giibWVG4=; 11, 48 -- b=B8tuc2/tVUWb+69n4B7+yAWfvmwpxGyj1PNAnMuLDJWfdRvVvTxe7m8ry/JQSXhj4c 11, 48 -- sC74hz/jOrvufUqg905aaBcVAo28jdGTDDVRSEu/CnL5Bv2gr7JeTeWkgkLHLd6qPeII 11, 48 -- 3DG2klvlhjblWaD6hMRRnEQnxYaGk9fRHNCpTHDw35igSh0tKD75mQepVSy0QEqmOKVo 11, 48 -- UuuHX6pC6s8DXHSKbe7dn3K+LfyKwwCti9o58LNRKLV7G7dHFILDkeseRO6ZtXtiCpkD 11, 48 -- /DFfUMl8mtMQYdg/y+F3x4yJhBm5HP340/ZRagbqYwTs0bG+d149UOvfkISykHkiZtKq 11, 48 -- QDVw== 11, 48 -- X-Gm-Message-State: AODbwcBfydcr2u9ovbLVy1Akyl75BKziUhLBJInIuDXEK7oJVwh6c8zz 11, 48 -- JxpDii8zQuSb823lg9pcsQ== 11, 48 -- X-Received: by 10.36.121.22 with SMTP id z22mr3912969itc.59.1494735071450; 11, 48 -- Sat, 13 May 2017 21:11:11 -0700 (PDT) 11, 48 -- Received: from AllenH.local (mobile-130-126-255-81.near.illinois.edu. [130.126.255.81]) 11, 48 -- by smtp.googlemail.com with ESMTPSA id y7sm2944257itc.27.2017.05.13.21.11.10 11, 48 -- for {Microscopy-at-microscopy.com} 11, 48 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 11, 48 -- Sat, 13 May 2017 21:11:10 -0700 (PDT) 11, 48 -- Message-ID: {5917D8DC.2060102-at-prairienanotech.com} 11, 48 -- Date: Sat, 13 May 2017 23:11:08 -0500 11, 48 -- From: Allen Hall {ajhall-at-prairienanotech.com} 11, 48 -- User-Agent: Postbox 5.0.13 (Macintosh/20170501) 11, 48 -- MIME-Version: 1.0 11, 48 -- To: Microscopy-at-microscopy.com 11, 48 -- Subject: Popular, Inspiring University of Illinois Scientist and Nanotechnology 11, 48 -- Pioneer Scott Maclaren Dies at 57 11, 48 -- X-Enigmail-Version: 1.2.3 11, 48 -- Content-Type: text/plain; charset=UTF-8 11, 48 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
==============================Original Headers============================== 21, 29 -- From donc-at-asmicro.com Tue May 16 15:22:40 2017 21, 29 -- Received: from resqmta-ch2-01v.sys.comcast.net (resqmta-ch2-01v.sys.comcast.net [69.252.207.33]) 21, 29 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v4GKMe6C000789 21, 29 -- for {microscopy-at-microscopy.com} ; Tue, 16 May 2017 15:22:40 -0500 21, 29 -- Received: from resomta-ch2-09v.sys.comcast.net ([69.252.207.105]) 21, 29 -- by resqmta-ch2-01v.sys.comcast.net with SMTP 21, 29 -- id Aj9ldh3eCO3QoAjAyd30eU; Tue, 16 May 2017 20:34:36 +0000 21, 29 -- Received: from asm20 ([68.58.83.137]) 21, 29 -- by resomta-ch2-09v.sys.comcast.net with SMTP 21, 29 -- id AjAwdMyN1jCS0AjAxdIn7u; Tue, 16 May 2017 20:34:36 +0000 21, 29 -- Message-ID: {A86A680FBF2B45228B859AC44AF9A6E1-at-asm20} 21, 29 -- From: "Don Chernoff at ASM" {donc-at-asmicro.com} 21, 29 -- To: "Microscopy List" {microscopy-at-microscopy.com} 21, 29 -- Cc: {ajhall-at-prairienanotech.com} , "Jon Goldman at ASM" {jon-at-asmicro.com} 21, 29 -- References: {201705140517.v4E5HERd024143-at-microscopy.com} 21, 29 -- Subject: Re: [a] [Microscopy] Popular, Inspiring University of Illinois Scientist and Nanotechnology 21, 29 -- Date: Tue, 16 May 2017 16:34:39 -0400 21, 29 -- MIME-Version: 1.0 21, 29 -- Content-Type: text/plain; 21, 29 -- format=flowed; 21, 29 -- charset="iso-8859-1"; 21, 29 -- reply-type=original 21, 29 -- Content-Transfer-Encoding: 8bit 21, 29 -- X-Priority: 3 21, 29 -- X-MSMail-Priority: Normal 21, 29 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5931 21, 29 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6157 21, 29 -- X-CMAE-Envelope: MS4wfO5svSPfMEyc/hGJd5nW+zvB+LMKlLOlRflppLJDfas3WZJVyAkz+y2qqj6dwL86RHU0PV9gC+JDKCPMIscdDafh4JZWIobF0ix6N7gL8TpwbVKtGSE8 21, 29 -- rcoeUaF2/rNlNeuzk56ISub2ljwlpiOZKBJWzuKrhHS3YMjVCNvArPhpfHtYL58SybX2Nq3vazkSlBaoLdiO2yVIFcDyOCHrauHXaE0dY37sdIr+CWey5Opj ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both zluo-at-uncfsu.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: zluo-at-uncfsu.edu Name: Zhiping Luo
Organization: Fayetteville State University
Title-Subject: [Filtered] EPMA/SEM staff position available
Message: A permanent staff position in EPMA/SEM is immediately available at Fayetteville State University, North Carolina.
Application should be submitted at https://jobs.uncfsu.edu/postings/15287.
More info can be found at http://www.uncfsu.edu/sencr-mic.
Login Host: 152.6.250.5 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
From irapier044-at-gmail.com Wed May 17 11:08:34 2017 Return-Path: {irapier044-at-gmail.com} Received: from gmail.com ([182.16.13.242]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v4HG8WDc025716 for {microscopylistserverarchive6-at-microscopy.com} ; Wed, 17 May 2017 11:08:33 -0500 Message-ID: {DB564461.A09262EF-at-gmail.com}
X-from: Gail.Phillips-at-umassmed.edu
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Gail.Phillips-at-umassmed.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Organization: University of Massachusetts Medical School
Title-Subject: [Filtered] Research Associate I - electron microscopy
Message: The Department of Neurobiology is seeking a research associate I with electron microscopy experience. Interested candidates should go to the link below and apply:
https://www.ummsjobs.com/job/2298/
Login Host: 146.189.55.18 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both tobi-at-stanford.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: tobi-at-stanford.edu Name: Tobi Beetz
Organization: Stanford University
Title-Subject: [Filtered] Job opening at Stanford University: Transmission Electron Microscopy Scientist, Stanford Nano Shared Facilities
Message: Transmission Electron Microscopy Scientist, Stanford Nano Shared Facilities
The Stanford Nano Shared Facilities (SNSF) is seeking a Transmission Electron Microscopy (TEM) scientist to lead the operations of the facilities FEI Titan Environmental TEM with spherical aberration corrector. The TEM scientist will operate, maintain, and optimize the microscope. He/She will provide training and support to researchers, develop and implement advanced techniques, and engage in research activities.
Stanfords shared nanofacilities offer a comprehensive array of advanced nanofabrication and nanocharacterization tools. Over 1,000 researchers make use of the shared facilities each year in order to further their research programs. The goal of the shared facilities at Stanford University is to provide open, cost-effective access to state-of-the-art nanofabrication and nanocharacterization facilities for scientists and engineers from academia, small and large companies, and government laboratories. The FEI Titan TEM is organized under SNSF in the Electron & Ion Microscopy Suite which currently features a FEI Tecnai TEM as well as two FIB/SEMs and two SEMs. The TEM scientist will report to the Faculty Director of SNSF. For more information about SNSF, visit http://snsf.stanford.edu.
Also still accepting applications for Scanning Probe Microscopy Laboratory Manager, Stanford Nano Shared Facilities, https://stanfordcareers.stanford.edu/job-search?jobId=74618
Login Host: 76.21.113.221 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both leandro.lemgrubersoares-at-glasgow.ac.uk as well as the Microscopy Listserver ---------------------------------------------------------------------------
Organization: Wellcome Centre for Molecular Parasitology - University of Glasgow
Title-Subject: [Filtered] ImageStream course
Message: Dear All
on August we will have a symposium here in Glasgow on the use of ImageStream in the fields of immunology and parasitology. To register and information about the event - https://www.eventbrite.co.uk/e/imagestream-symposium-tickets-32725310284
Feel free to advertise within your institutions.
Best
Leandro
Login Host: 130.209.127.194 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ravithakkar-at-vet.k-state.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Message: Hi Listeners, If any body here has used K-kit to analyse liquid sample under TEM. Is k-kit useful for liposome too? What kind of specimen holder needed? Will FEI Double tilt holder works?
Ravi.
Login Host: 129.130.145.42 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
(1) If anybody here has used K-kit to analyze liquid sample under TEM.
We have worked with the K-kit system in our facility as well as working with others who are using the K-kit for a number of liquid systems.
(2) Is k-kit useful for liposome too?
In general, if a k-kit loaded with organic substance that without staining, the imaging results mostly would suffer low contrast under TEM. The liposome is just one of the examples.
A liposome has an aqueous solution core surrounded by a thin hydrophobic membrane, in the form of a lipid bilayer. Due to the contrast issue, the TEM images for some smaller sizes of liposomes are easily looked blurry, and poor for being clearly identified. Per our actual test results, we suggest that Cryo-TEM can be a better option for those smaller liposomes. However, one also can use K-kits for the observations of some larger liposomes under TEM.
(3) What kind of specimen holder needed?
Since K-kit is mounted on a standard copper grid for TEM observation, it's sure compatible with most kinds of TEM Holder.
(4) Will FEI Double tilt holder works?
Some kinds of FEI double tilt holders require using a small screwed cover ring to lock the copper grid fixed at the holder's front stage. It usually needs to use a dedicated tool which with a small hexagonal head to fasten them together. In this case, due to K-kit body on the copper grid is too thick, it will make the tool failed for screwing down the cover ring.
I hope this information has been of some help.
Regards
Gene Rodek
Eugene Rodek Vice President SPI Supplies 206 Garfield Ave, West Chester, PA 19380 610-436-5400 (P), 610-436-5755 (F) erodek-at-2spi.com www.2spi.com
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Friday, May 19, 2017 7:22 PM To: Gene Rodek {erodek-at-2spi.com}
X-from: ravithakkar-at-vet.k-state.edu
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ravithakkar-at-vet.k-state.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Message: Hi Listeners, If any body here has used K-kit to analyse liquid sample under TEM. Is k-kit useful for liposome too? What kind of specimen holder needed? Will FEI Double tilt holder works?
Ravi.
Login Host: 129.130.145.42 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
I defended my Ph.D. thesis in October 2016 and now I am looking for a postdoctoral position in microscopy (AFM, TEM, SEM) and biophysics of microorganisms(especially, viruses, I like them :)). If there is an open position in your lab, please write me.
Profile in ResearchGate https://www.researchgate.net/profile/Denis_Korneev
Profile in Google Scholar http://scholar.google.ch/citations?user=xW-kLNoAAAAJ&hl
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both dcristofori-at-unive.it as well as the Microscopy Listserver ---------------------------------------------------------------------------
Login Host: 133.16.169.10 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both fitzp-at-wustl.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: fitzp-at-wustl.edu Name: James Fitzpatrick
Organization: Washington University School of Medicine
I have a new position open in my group for a Cryo-Electron Microscopy Specialist. This role will support the operation of my new Titan Krios G3 Cryo-EM which is equipped with a K2 Direct Electron Detector for single particle averaging and cellular tomography studies. The position will work with other EM staff under my supervision in operation of the electron microscopy component of my group - Wash U Center for Cellular Imaging (http://wucci.wustl.edu/). The Cryo-EM Specialist will also have the opportunity to work on correlative microscopy technology development projects with other members of the group.
PRIMARY DUTIES AND RESPONSIBILITIES:
User Training - train users in sample preparation and imaging techniques for single-particle cryo-EM.
Cryo-EM Service - Prepare samples for single particle cryo-EM and collect and reconstruct images for University researchers to help to prepare data for publication and presentations.
Cryo-EM Equipment Maintenance - maintenance of the cryo-EM and sample preparation equipment.
QUALIFICATIONS
At least 2-5 years of cryo-EM experience, with expertise in sample freezing, sample handling, microscope use, and data evaluation and analysis.
Firm understanding of the principles of Transmission Electron Microscopy (TEM)
Demonstrated skill and experience in the high-resolution operation of cryo electron microscopes
General understanding of single-particle cryo-EM workflow, experience in cryo-electron tomography, Cs correction and Phase contrast systems would be added benefits.
Proven organizational and time management skills to successfully set priorities, meet established deadlines and recognize new problems with constantly changing priorities and frequent interruptions.
Strong communication skills (both verbal and written) needed to interact professionally and effectively in the work environment. Ability to read, comprehend, and discuss research materials. Proven ability to write, edit and proofread research results. Skill at explaining difficult concepts and training users and students.
Ability to diplomatically and professionally interact with all levels of University faculty and staff and external contacts.
Proven ability to perform minor maintenance and alignment of electron microscopes.
APPLICATION INSTRUCTIONS
Please go to: jobs.wustl.edu and search for job ID 36721 Research Specialist (Cryo-Electron Microscopy) - Center for Cellular Imaging
Login Host: 128.252.79.225 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both steve.sem-at-icloud.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: steve.sem-at-icloud.com Name: Steve Perry
Organization: SEM Lab support
Title-Subject: [Filtered] Pemtron Pemscan SEM technical Message: I'm looking for any service information for the Pemscan PS-230 SEM. Anything like the engineer login, schematics or parts diagrams would be helpful. This SEM looks and acts like an ISI sold under a new name.
Thanks in Advance,
Steve Perry
Login Host: 174.76.17.36 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both stefano-at-soquelec.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Job opportunity as electron microscopy sales and service representative
Message: Start date: ASAP Hourly/yearly wage: To be discussed Job type: Permanent Relocation/travel requirements: up to 80% travelling Soquelec Limited, founded in 1974, is an established distributor of high-end imaging analysis equipment, sample preparation equipment, and consumables for life and material science laboratories across Canada. Job Description We are looking for an industrious and self-driven sales and service representative to represent our company in Ontario. You will be responsible for the sales and service of scientific equipment from a variety of suppliers (TESCAN, Gatan, Bruker, Quorum, etc.), as well as establish and maintain strong business relationships with our customers. You will be working with the rest of the sales team and the administrative staff closely to achieve your sales goals. The ideal candidate should have at least a Bachelors Degree in Science, Engineering or related field, a drivers license and a passport for international travel. You will also need to have excellent communication skills in English; a working knowledge of French would be preferred. The position entails frequent travels to our customers locations in Canada and to supplier meetings and training sessions in the USA or Europe. Key Responsibilities Initializing contacts with potential customers and qualifying sales opportunities On-site presentations and/or demonstrations of scientific equipment Following up and closing sales opportunities in a timely manner Installation and/or servicing instruments Training end users of said instruments Updating CRM according to the sales pipeline Participating in applicable trade shows and conferences Requirements Bachelors Degree in Science or Engineering Excellent communication skills in English Drivers license Willing and ready to travel frequently Ability to identify customers needs and follow up accordingly Self-motivation and enthusiasm Team player Assets Experience in electron microscopy or x-ray imaging Previous sales/retail/customer service experience Familiarity with Microsoft Dynamics CRM Working knowledge of French
Login Host: 38.121.70.205 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
From kipemilf56-at-gmail.com Fri May 26 23:49:40 2017 Return-Path: {kipemilf56-at-gmail.com} Received: from gmail.com ([222.97.39.119]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v4R4nbn2014324 for {microscopylistserverarchive6-at-microscopy.com} ; Fri, 26 May 2017 23:49:39 -0500 Message-ID: {57AF2145.6330572C-at-gmail.com}
X-from: mibuckett-at-mmm.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mibuckett-at-mmm.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: mibuckett-at-mmm.com Name: Mary Buckett
Organization: 3M Co.
Title-Subject: [Filtered] Job Opening - TEM Specialist
Message: 3M is seeking a TEM Research Specialist for the Corporate Research Analytical Laboratory (CRAL) located in Maplewood, MN. Job Summary: The person hired for the position of Research Specialist (Transmission Electron Microscopy) will partner with corporate laboratories as well as division R&D and manufacturing in the development of technologies and products.
Preferred Qualifications: Ph.D. in Materials Science, Polymer Science, Chemistry, Chemical Engineering, Physics or related discipline from an accredited university.
More information and job application can be found at: https://3m.wd1.myworkdayjobs.com/en-US/Search/job/US-Minnesota-Maplewood/Research-Specialist-Transmission-Electron-Microscopy---Maplewood--MN-_R00031146-1
Login Host: 108.171.131.181 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both stacie-at-ems-secure.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Message: EMS Microscopy Academy announces the Cryo SEM Workshop in July. This course will cover the process of rapid freezing, fracturing, coating and imaging of a variety of samples. For more information, see http://ow.ly/WzPe30bmk8v
Login Host: 73.165.127.32 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both YaqiaoWu-at-boisestate.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: YaqiaoWu-at-boisestate.edu Name: Yaqiao Wu
Organization: Boise State University/Center for Advanced Energy Studies
Title-Subject: [Filtered] Job opening: Senior Research Associate - Electron Microscopy
Message: Dear colleagues,
Micron School of Materials, Science and Engineering at Boise State University is looking for candidates for the position of Senior Research Associate in electron microscopy.
To apply online, please go to https://boisestate.taleo.net/careersection/ex/jobdetail.ftl?job=170442&tz=GMT-06:00
Job description details:
Position Overview
Boise State University, powered by creativity and innovation, stands uniquely positioned in the Northwest as a metropolitan research university of distinction. The Center for Advanced Energy Studies (CAES) seeks candidates for the position of Senior Research Associate.
This position works in a multi-collaborative environment at the Center for Advanced Energy Studies in Idaho Falls, Idaho, and assists Boise State University, Idaho State University, University of Idaho, University of Wyoming, Idaho National Laboratories, and external customers with conducting research in both the Microstructure and Characterization Suite (MaCS) and Advanced Materials Laboratory (AML). Responsibilities include sample preparation, microstructure characterization and chemical analysis utilizing Focused-Ion Beam (FIB), Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM), involvement in materials research projects; handle radioactive materials.
You will have the opportunity to: Assist with sample preparation on the bench top and inside of a glovebox using traditional cutting and polishing methods as well as advanced techniques including FIB, ion milling and coating deposition; Perform characterization of materials including electron microscopy (FIB/SEM, TEM) and chemical analysis; train new users on the equipment as needed; Operate equipment and conduct experiments in the Advanced Materials Laboratory including spark plasma sintering, heat treatments, and mechanical testing; Interact with customers, assessing problems, and assisting in solving materials related problems; Safely handle, store, and dispose of radioactive materials on a daily basis; Maintain compliance with safety, security, and environmental regulations on the safe handling of chemicals and radioactive materials; Trouble shoot issues and coordinate the repair of equipment/tools as needed. Qualifications At a minimum you should have: A Bachelors degree in materials science or related discipline and 8 years of experience in a similar type of work that includes the following: An understanding of energy materials and high temperature processing equipment; Experience in sample preparation techniques; Experience in electron microscopy techniques; Ability to translate, adapt and apply academic and/or practical knowledge to engineering research; Ability to instruct others on instrument use, scientific principles, and safety; Excellent oral and written communication skills; Ability to work with a diverse group of people; Knowledge of good safety practices. Preferentially, you will have: Masters degree in materials science or related discipline, with a minimum of 6 years of experience in a materials research laboratory that includes the following: Experience in safe handling and managing of radioactive materials; Experience in working in a glovebox environment; Experience in advanced characterization/chemical analysis techniques including FIB/SEM, TEM, EBSD and EDS; Proficiency in the maintenance and use of advanced materials processing techniques.
Salary and benefits: Salary is commensurate with experience. An excellent benefits package is available for eligible employees, for more information visit: http://hrs.boisestate.edu/careers/benefits/. If you are interested in this position: Please apply online and upload a cover letter and resume (including dates of employment) and the names of three professional references with contact information. Review of documents will begin immediately. The deadline to apply is June 16, 2017.
Login Host: 8.23.154.5 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both pscallio-at-dal.ca as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: pscallio-at-dal.ca Name: Pat Scallion
Organization: Dalhousie University
Title-Subject: [Filtered] Hitachi S4700: air sensitive sample
Message: Hi, I have a user with a sample that must be kept under an Argon atmosphere until placed in the SEM exchange chamber, and I have never had to deal with this situation before.I am wondering if the following procedure will work, or if there are any issues with it.
Our SEM has a separate exchange chamber prior to the Sample chamber. We propose to tape a flexible glove bag around the frame of the SEC, containing the samples and mounts. We would first evacuate the bag using the rotary pumps, then seal the exchange chamber. Then, we will flush the glove bag several times with argon using the glove bag ports only.
Once the argon atmosphere is present, the samples will be removed from their carrier and placed on the SEM mount in the glove bag, then into the exchange chamber and evacuated. Once vacuum is reached the sample will be put into the sample chamber. Hopefully all the argon will be gone by then.
After viewing, there is no current plan to try and keep the samples from the atmosphere.
Also, our SEM is used by over 90 researchers from several faculties. Would a temporary set-up like this with the argon cause any problems or concerns down the road?
As I said, this is the first time for me trying something like this, and I don't want any surprises.
Thanks in advance for your help!
Pat Scallion
Login Host: 129.173.116.16 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both miketoalson-at-gmail.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: miketoalson-at-gmail.com Name: Mike Toalson
Organization: NanoImages, LLC
Title-Subject: [Filtered] viaWWW:Hitachi S4700: air sensitive sample
Message: Hello Pat,
Your concept sounds feasible but I'll let the experts on here comment further.
I thought you might want to have a look at 2 other solutions that I have seen:
1) VacuShut sample transfer device - this was sold by Agar Scientific but I don't see it on their website now. It was invented at Karlsruhe Inst for Tech. Here is a flyer PDF showing how it works. Maybe the inventors listed on this know where you can acquire it now: http://www.int.kit.edu/downloads/INT_Research/Flyervacushut.pdf 2) Transfer Module from Kammrath Weiss - a more elegant solution (and probably more expensive) https://www.kammrath-weiss.com/en/products/materials/transfer-module.html Good Luck!!
Mike Toalson NanoImages, LLC t 866.601.6266 m 530.691.3180 e mtoalson-at-nanoimages.com web http://www.nanoimages.com/ skype mike.toalson
} From: mailto:microscopy.listserver-at-gmail.com Sent: Tuesday, May 30, 2017 6:31 PM To: mailto:miketoalson-at-gmail.com
X-from: mailto:pscallio-at-dal.ca
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mailto:pscallio-at-dal.ca as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: mailto:pscallio-at-dal.ca Name: Pat Scallion
Organization: Dalhousie University
Title-Subject: [Filtered] Hitachi S4700: air sensitive sample
Message: Hi, I have a user with a sample that must be kept under an Argon atmosphere until placed in the SEM exchange chamber, and I have never had to deal with this situation before.I am wondering if the following procedure will work, or if there are any issues with it.
Our SEM has a separate exchange chamber prior to the Sample chamber. We propose to tape a flexible glove bag around the frame of the SEC, containing the samples and mounts. We would first evacuate the bag using the rotary pumps, then seal the exchange chamber. Then, we will flush the glove bag several times with argon using the glove bag ports only.
Once the argon atmosphere is present, the samples will be removed from their carrier and placed on the SEM mount in the glove bag, then into the exchange chamber and evacuated. Once vacuum is reached the sample will be put into the sample chamber. Hopefully all the argon will be gone by then.
After viewing, there is no current plan to try and keep the samples from the atmosphere.
Also, our SEM is used by over 90 researchers from several faculties. Would a temporary set-up like this with the argon cause any problems or concerns down the road?
As I said, this is the first time for me trying something like this, and I don't want any surprises.
Thanks in advance for your help!
Pat Scallion
Login Host: 129.173.116.16 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
Login Host: 47.208.204.209 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
It sounds like something I did back in 2002 on a similar SEM back at the Electron Microscopy Center at Argonne National Lab. Should work just fine and an inert gas like argon will not cause any problems. Science and research are all about having fun and trying new things.
Enjoy! Roseann
Roseann Csencsits President Northern California Society for Microscopy Group Leader-Microbeam Analyses Schafer Vallecitos Laboratories, Inc. a Belcan company 6705 Vallecitos Rd. Sunol, CA 94586 925-862-4345 roseann.csencsits-at-schafercorp.com
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
X-from: pscallio-at-dal.ca
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both pscallio-at-dal.ca as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: pscallio-at-dal.ca Name: Pat Scallion
Organization: Dalhousie University
Title-Subject: [Filtered] Hitachi S4700: air sensitive sample
Message: Hi, I have a user with a sample that must be kept under an Argon atmosphere until placed in the SEM exchange chamber, and I have never had to deal with this situation before.I am wondering if the following procedure will work, or if there are any issues with it.
Our SEM has a separate exchange chamber prior to the Sample chamber. We propose to tape a flexible glove bag around the frame of the SEC, containing the samples and mounts. We would first evacuate the bag using the rotary pumps, then seal the exchange chamber. Then, we will flush the glove bag several times with argon using the glove bag ports only.
Once the argon atmosphere is present, the samples will be removed from their carrier and placed on the SEM mount in the glove bag, then into the exchange chamber and evacuated. Once vacuum is reached the sample will be put into the sample chamber. Hopefully all the argon will be gone by then.
After viewing, there is no current plan to try and keep the samples from the atmosphere.
Also, our SEM is used by over 90 researchers from several faculties. Would a temporary set-up like this with the argon cause any problems or concerns down the road?
As I said, this is the first time for me trying something like this, and I don't want any surprises.
Thanks in advance for your help!
Pat Scallion
Login Host: 129.173.116.16 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
=========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
Microscopy-at-Microscopy.com
============================================
==============================Original Headers============================== 26, 59 -- From roseann.csencsits-at-schafercorp.com Wed May 31 07:05:41 2017 26, 59 -- Received: from server559.appriver.com (server559b.appriver.com [8.19.118.63]) 26, 59 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v4VC5fGo006509 26, 59 -- for {microscopy-at-microscopy.com} ; Wed, 31 May 2017 07:05:41 -0500 26, 59 -- X-Note: This Email was scanned by AppRiver SecureTide 26, 59 -- X-Note-AR-ScanTimeLocal: 05/31/2017 7:18:31 AM 26, 59 -- X-Note: SecureTide Build: 4/27/2017 5:11:48 PM UTC (2.6.18.8) 26, 59 -- X-Note: Filtered by 10.87.0.103 26, 59 -- X-Policy: schafercorp.com - schafercorp.com 26, 59 -- X-Policy: schafercorp.com - schafercorp.com 26, 59 -- X-Primary: roseann.csencsits-at-schafercorp.com 26, 59 -- X-Virus-Scan: V- 26, 59 -- X-Note: ICH-CT/SI:0-875/SG:1 5/31/2017 7:18:28 AM 26, 59 -- X-Note-SnifferID: 0 26, 59 -- X-Note: TCH-CT/SI:0-94/SG:2 5/31/2017 7:18:28 AM 26, 59 -- X-GBUdb-Analysis: 0, 104.130.106.14, Ugly c=0.550035 p=-0.932692 Source Normal 26, 59 -- X-Signature-Violations: 26, 59 -- 0-0-0-7965-c 26, 59 -- X-Note-419: 31.2723 ms. Fail:0 Chk:1364 of 1364 total 26, 59 -- X-Note: VSCH-CT/SI: 0-1364/SG:1 5/31/2017 7:18:28 AM 26, 59 -- X-Note: Spam Tests Failed: OPTOUT 26, 59 -- X-Country-Path: PRIVATE-} -} United States-} 26, 59 -- X-Note-Sending-IP: 104.130.106.14 26, 59 -- X-Note-Reverse-DNS: mail3.schafercorp.com 26, 59 -- X-Note-Return-Path: roseann.csencsits-at-schafercorp.com 26, 59 -- X-Note: User Rule Hits: 26, 59 -- X-Note: Global Rule Hits: G269 G270 G271 G272 G276 G277 G372 G405 26, 59 -- X-Note: Encrypt Rule Hits: 26, 59 -- X-Note: Mail Class: VALID 26, 59 -- X-Note: Headers Injected 26, 59 -- Received: from [104.130.106.14] (HELO mail.schafercorp.com) 26, 59 -- by server559.appriver.com (CommuniGate Pro SMTP 6.1.7) 26, 59 -- with ESMTPS id 244534982; Wed, 31 May 2017 07:18:31 -0500 26, 59 -- Received: from 822060-exch4.schafer.lan (192.168.100.15) by 26, 59 -- 822017-exch3.schafer.lan (192.168.100.14) with Microsoft SMTP Server (TLS) id 26, 59 -- 15.0.1210.3; Wed, 31 May 2017 07:18:30 -0500 26, 59 -- Received: from 822060-exch4.schafer.lan ([192.168.100.15]) by 26, 59 -- 822060-exch4.schafer.lan ([192.168.100.15]) with mapi id 15.00.1210.000; Wed, 26, 59 -- 31 May 2017 07:18:30 -0500 26, 59 -- From: "Csencsits, Roseann" {roseann.csencsits-at-schafercorp.com} 26, 59 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 26, 59 -- CC: "pscallio-at-dal.ca" {pscallio-at-dal.ca} 26, 59 -- Subject: RE: [Microscopy] viaWWW:Hitachi S4700: air sensitive sample 26, 59 -- Thread-Topic: [Microscopy] viaWWW:Hitachi S4700: air sensitive sample 26, 59 -- Thread-Index: AQHS2a1viU9ejK5TL0+oA+jJ3dM9o6IOWh9A 26, 59 -- Date: Wed, 31 May 2017 12:18:30 +0000 26, 59 -- Message-ID: {6b9f7c021b124befa0e86356ebb2362f-at-822060-exch4.schafer.lan} 26, 59 -- References: {201705310117.v4V1HGDU018926-at-microscopy.com} 26, 59 -- In-Reply-To: {201705310117.v4V1HGDU018926-at-microscopy.com} 26, 59 -- Accept-Language: en-US 26, 59 -- Content-Language: en-US 26, 59 -- X-MS-Has-Attach: 26, 59 -- X-MS-TNEF-Correlator: 26, 59 -- x-ms-exchange-transport-fromentityheader: Hosted 26, 59 -- x-originating-ip: [192.168.99.192] 26, 59 -- Content-Type: text/plain; charset="iso-8859-1" 26, 59 -- MIME-Version: 1.0 26, 59 -- Content-Transfer-Encoding: 8bit 26, 59 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v4VC5fGo006509 ==============================End of - Headers==============================
Zach L. Rowland Director Institute for Imaging and Analytical Technologies (I2AT) Mississippi State University 200 Research Blvd, Starkville, MS 39759
P.O. Box 5405 Mississippi State. MS 39762
Off - 662-325-1607 Cell 662-341-0397 Fax -662-325-5433 zrowland-at-i2at.msstate.edu
==============================Original Headers============================== 9, 32 -- From zrowland-at-i2at.msstate.edu Wed May 31 17:17:05 2017 9, 32 -- Received: from catalpa.its.msstate.edu (catalpa.its.msstate.edu [130.18.2.119]) 9, 32 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v4VMH4uJ023365 9, 32 -- for {Microscopy-at-Microscopy.Com} ; Wed, 31 May 2017 17:17:04 -0500 9, 32 -- Received: from mail01.ad.msstate.edu (mail01.ad.msstate.edu [130.18.230.60]) 9, 32 -- by catalpa.its.msstate.edu (8.13.8/8.13.8) with ESMTP id v4VMTuCB006806 9, 32 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=FAIL) 9, 32 -- for {Microscopy-at-Microscopy.Com} ; Wed, 31 May 2017 17:29:56 -0500 9, 32 -- X-Sender: {} 9, 32 -- Received: from MAIL01.ad.msstate.edu (2620:0:1a30:1230:85cd:146c:55d4:e430) by 9, 32 -- mail01.ad.msstate.edu (2620:0:1a30:1230:85cd:146c:55d4:e430) with Microsoft 9, 32 -- SMTP Server (TLS) id 15.0.1236.3; Wed, 31 May 2017 17:29:55 -0500 9, 32 -- Received: from MAIL01.ad.msstate.edu ([fe80::85cd:146c:55d4:e430]) by 9, 32 -- mail01.ad.msstate.edu ([fe80::85cd:146c:55d4:e430%14]) with mapi id 9, 32 -- 15.00.1236.000; Wed, 31 May 2017 17:29:55 -0500 9, 32 -- From: "Rowland, Zach" {zrowland-at-i2at.msstate.edu} 9, 32 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com} 9, 32 -- Subject: Job Opportunity - Mississippi State University 9, 32 -- Thread-Topic: Job Opportunity - Mississippi State University 9, 32 -- Thread-Index: AdLaXGC4QpLa21j1R3iKsThfBzFGNA== 9, 32 -- Date: Wed, 31 May 2017 22:29:54 +0000 9, 32 -- Message-ID: {a3775f14732d4ba88b9b72b5184e3758-at-mail01.ad.msstate.edu} 9, 32 -- Accept-Language: en-US 9, 32 -- Content-Language: en-US 9, 32 -- X-MS-Has-Attach: 9, 32 -- X-MS-TNEF-Correlator: 9, 32 -- x-ms-exchange-transport-fromentityheader: Hosted 9, 32 -- x-originating-ip: [130.18.230.93] 9, 32 -- Content-Type: text/plain; charset="iso-8859-1" 9, 32 -- MIME-Version: 1.0 9, 32 -- Content-Transfer-Encoding: 8bit 9, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v4VMH4uJ023365 ==============================End of - Headers==============================
From whitejoye45-at-gmail.com Wed May 31 18:53:17 2017 Return-Path: {whitejoye45-at-gmail.com} Received: from gmail.com ([182.16.37.130]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v4VNrEK6017333 for {microscopylistserverarchive6-at-microscopy.com} ; Wed, 31 May 2017 18:53:16 -0500 Message-ID: {79C4AB01.C20B0056-at-gmail.com}
I'll have to replace my LMIS soon and was just wondering what they start as. Seems like the less pure, the less ideal your focused beam spot would become, more 'chromatic' aberration probably. Just looking around I find 99.99% pure through 99.99995% available... so where would these be relative to what precision FIB would commonly use?
I'd guess that for some applications, a custom ion mill could tolerate a wide range of purity (not that I can imagine one at the moment). It leads to the question of, do all impurities ionize, or would some remain as 'slag' that prevents transport (flow) of clean metal to the emission point.
When I was at Drexel, I remember the FIB scientist discovered (with the help of another scientist at Applied Beams) a solution as to why all of our ion beam images and milling patterns had these ghostly shadows present. For example, if you tried to drill a very small hole into a silicon nitride membrane, instead of single hole you would get two! This turned out to be because our ion source had two different isotopes of gallium present, and each isotope experienced a slightly different path through the optics and was focused to a crossover that was slightly laterally displaced with respect to each other. The purity of the source is certainly one thing to consider but apparently the isotopic distribution of even a pure gallium source is another. This doesn't answer your question directly but I thought you might find it interesting. I imagine the FIB source suppliers go to some extra lengths the ion source is isotopically pure Ga-69...
Cheers, Chris
On Thu, Jun 1, 2017 at 1:55 PM, {nmz787-at-gmail.com} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I'll have to replace my LMIS soon and was just wondering what they } start as. Seems like the less pure, the less ideal your focused beam } spot would become, more 'chromatic' aberration probably. } Just looking around I find 99.99% pure through 99.99995% available... } so where would these be relative to what precision FIB would commonly } use? } } I'd guess that for some applications, a custom ion mill could tolerate } a wide range of purity (not that I can imagine one at the moment). It } leads to the question of, do all impurities ionize, or would some } remain as 'slag' that prevents transport (flow) of clean metal to the } emission point. } } -- } -Nathan } } ==============================Original Headers============================== } 3, 38 -- From nmz787-at-gmail.com Thu Jun 1 12:45:17 2017 } 3, 38 -- Received: from mail-pf0-f178.google.com (mail-pf0-f178.google.com [209.85.192.178]) } 3, 38 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v51HjHnE012745 } 3, 38 -- for {Microscopy-at-microscopy.com} ; Thu, 1 Jun 2017 12:45:17 -0500 } 3, 38 -- Received: by mail-pf0-f178.google.com with SMTP id m17so33630647pfg.3 } 3, 38 -- for {Microscopy-at-microscopy.com} ; Thu, 01 Jun 2017 10:58:12 -0700 (PDT) } 3, 38 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 3, 38 -- d=gmail.com; s=20161025; } 3, 38 -- h=mime-version:from:date:message-id:subject:to; } 3, 38 -- bh=b63n8IsrRB4iEn0ibUFgxzF312k7QNITfPoc9ZI+65I=; } 3, 38 -- b=dnDOhzDZX7PT/hj7Fqp0TOsQwm9jgQhlTYuaCVh1Pc33EVN3TiOVo5SRfffx9SEoG1 } 3, 38 -- q9GQ0sdwAjW80nrcON9rtlrKurFZcaMVSUXJXGOUOZLzVqnLv5AsvkSESenAPW44S5Cs } 3, 38 -- s1rOBf/qw79d9NsyYCZ3bK2etHqjV0KoOrCuEIt/rzeQeBanVAzo9/RA4JEZY9Zt4E1F } 3, 38 -- IuWxUcaZsKQZZnNSMQsXITyxzPiv2OI+jd35WFDREi5LnHNZJxCGsKhMYueyONZAa2B3 } 3, 38 -- oFrL4S7ApPUI7eNYIYSXLzTifu/gCndJfMIvWpDNFTSQfiLHZ/iwQ0aVUQUuzAe3rnrN } 3, 38 -- lzWA== } 3, 38 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 3, 38 -- d=1e100.net; s=20161025; } 3, 38 -- h=x-gm-message-state:mime-version:from:date:message-id:subject:to; } 3, 38 -- bh=b63n8IsrRB4iEn0ibUFgxzF312k7QNITfPoc9ZI+65I=; } 3, 38 -- b=Pnq6SMvOogAUxD8xLOt2YuW41SDmC7HCp1jxiHTVOzRYwrEjrVncZDcvt1SdjYseIn } 3, 38 -- QMe0dAznrlmp1mlS61X8oisNKwvfyh2rEpX6VhDK9P/sDtiOqzPgoQCbkp4qXuolbykR } 3, 38 -- okxWLZqH4eaUgl53PgDcpIegmEnt+R3V7pgVxYN/NKelPV1CUUhe/1YelEzTJDvkBPL5 } 3, 38 -- Gtjd6dHn8xYaDlyDXNVYzBZul1/2l8i6ujnI8vMocFLv+UjEpk3ITaisAnYAtRmAlgQE } 3, 38 -- caiCWXTOWz71U2O067c8g7mSrC6ymC0S8I+KoVaTafL0fHSkQ6qjfVMlF86WU6w8tN2l } 3, 38 -- klPg== } 3, 38 -- X-Gm-Message-State: AODbwcBAjKwnNW9a3jdiRvxr0ADHwnDQMJX+5jlpMIurt0QG6n0tLdgk } 3, 38 -- hrD3FenW9ENSBbfBfNZEJkgP2/MNXA== } 3, 38 -- X-Received: by 10.84.193.129 with SMTP id f1mr97894164pld.129.1496339891072; } 3, 38 -- Thu, 01 Jun 2017 10:58:11 -0700 (PDT) } 3, 38 -- MIME-Version: 1.0 } 3, 38 -- Received: by 10.100.164.234 with HTTP; Thu, 1 Jun 2017 10:57:49 -0700 (PDT) } 3, 38 -- From: Nathan McCorkle {nmz787-at-gmail.com} } 3, 38 -- Date: Thu, 1 Jun 2017 10:57:49 -0700 } 3, 38 -- Message-ID: {CA+82U9KijoKasdHuoai3jRi-sZe6UPNpWpALnUO1gDc_vNGTpA-at-mail.gmail.com} } 3, 38 -- Subject: Typical metallic purity for FIB source? } 3, 38 -- To: Microscopy-at-microscopy.com } 3, 38 -- Content-Type: text/plain; charset="UTF-8" } ==============================End of - Headers==============================
From bjzhengqs-at-ruihesoft.com Thu Jun 1 22:00:57 2017 Return-Path: {bjzhengqs-at-ruihesoft.com} Received: from mail.ruihesoft.com (mail.ruihesoft.com [112.65.245.173]) by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v5230uAA021811; Thu, 1 Jun 2017 22:00:57 -0500 Message-Id: {201706020300.v5230uAA021811-at-microscopy.com} Received: from User (unknown [103.79.141.38]) by mail.ruihesoft.com (EMOS V1.6 (Postfix)) with ESMTPA id 1BC3A22D5B; Fri, 2 Jun 2017 06:36:23 +0800 (CST) Reply-To: {sarahadams212-at-yandex.com}
Dear colleagues,
We would like to remind you the Yale Microscopy Workshop which will be held on June 6 & 7, 2017.
We invite you to participate in a two day workshop. This free annual event is a combination of symposium, demonstrations, technical lectures and small group practicals. It is open to the international research community and only requires your free registration.
Time: June 6 - 7th, 2017 Location: Yale University School of Medicine, The Anlyan Center (TAC), New Haven, CT Cost: Free on-line or on-site registration Information: https://medicine.yale.edu/lab/microscopy.
Features: - Symposia on image analysis and high-dimensional imaging - Concurrent workshop on correlative live-cell STED and cryo-electron microscopy, and cryo EM techniques - Demonstrations, technical lectures, and small group practicals.
Please see the website for free registration, list of accessible equipment, and the schedule of events.
We look forward to seeing you there,
The organizers, Ann Haberman ann.haberman-at-yale.edu {mailto:ann.haberman-at-yale.edu} Derek Toomre derek.toomre-at-yale.edu {mailto:derek.toomre-at-yale.edu} Joerg Bewersdorf joerg.bewersdorf-at-yale.edu {mailto:joerg.bewersdorf-at-yale.edu} Xinran Liu xinran.liu-at-yale.edu {mailto:xinran.liu-at-yale.edu}
From mail.3185.educanet2.ch-at-smtp.bestone.cz Fri Jun 2 15:43:35 2017 Return-Path: {mail.3185.educanet2.ch-at-smtp.bestone.cz} Received: from smtp.bestone.cz (smtp.bestone.cz [193.19.176.35]) by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v52KhYvS013931; Fri, 2 Jun 2017 15:43:35 -0500 Message-Id: {201706022043.v52KhYvS013931-at-microscopy.com} Received: from TerminalKampen.post.lokaal (dsl-083-247-060-087.solcon.nl [83.247.60.87]) (Authenticated sender: test-at-nativ.cz) by smtp.bestone.cz (Postfix) with ESMTPA id 9E5B3222AB; Fri, 2 Jun 2017 22:16:25 +0200 (CEST) Content-Type: text/plain; charset="iso-8859-1" MIME-Version: 1.0 Content-Description: Mail message body
Hello,
I am trying to figure out what part of the cellular organelle or molecules, the ethanolic phosphotungstic acid (E-PTA) binds to show the electron dense staining? I have found that its commonly used for visualizing phospholipids or nerve ending. However, I dont know if E-PTA binds to lipids molecules or membranes. Does anyone know exactly what E-PTA binds to?
Thank you!
Sincerely,
Kyoung Jo
==============================Original Headers============================== 6, 48 -- From Kyoung.Jo-at-rockets.utoledo.edu Mon Jun 5 17:07:51 2017 6, 48 -- Received: from NAM03-CO1-obe.outbound.protection.outlook.com (mail-co1nam03on0056.outbound.protection.outlook.com [104.47.40.56]) 6, 48 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v55M7ot1006755 6, 48 -- for {Microscopy-at-microscopy.com} ; Mon, 5 Jun 2017 17:07:51 -0500 6, 48 -- Received: from BN6PR01MB2449.prod.exchangelabs.com (10.173.139.147) by 6, 48 -- BN6PR01MB2449.prod.exchangelabs.com (10.173.139.147) with Microsoft SMTP 6, 48 -- Server (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_128_CBC_SHA256_P256) id 6, 48 -- 15.1.1143.10; Mon, 5 Jun 2017 22:21:00 +0000 6, 48 -- Received: from BN6PR01MB2449.prod.exchangelabs.com ([10.173.139.147]) by 6, 48 -- BN6PR01MB2449.prod.exchangelabs.com ([10.173.139.147]) with mapi id 6, 48 -- 15.01.1143.018; Mon, 5 Jun 2017 22:21:00 +0000 6, 48 -- From: "Jo, Kyoung Ha" {Kyoung.Jo-at-rockets.utoledo.edu} 6, 48 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 6, 48 -- Subject: TEM need help on staining methods 6, 48 -- Thread-Topic: TEM need help on staining methods 6, 48 -- Thread-Index: AQHS3klYTql8PBAXJUCSiC7iSH6a9w== 6, 48 -- Date: Mon, 5 Jun 2017 22:21:00 +0000 6, 48 -- Message-ID: {BN6PR01MB24494491BB2CFF5EB6CF5120BFCA0-at-BN6PR01MB2449.prod.exchangelabs.com} 6, 48 -- Accept-Language: en-US 6, 48 -- Content-Language: en-US 6, 48 -- X-MS-Has-Attach: 6, 48 -- X-MS-TNEF-Correlator: 6, 48 -- authentication-results: microscopy.com; dkim=none (message not signed) 6, 48 -- header.d=none;microscopy.com; dmarc=none action=none 6, 48 -- header.from=rockets.utoledo.edu; 6, 48 -- x-originating-ip: [2603:10b6:400:4:cafe::a9] 6, 48 -- x-ms-publictraffictype: Email 6, 48 -- x-microsoft-exchange-diagnostics: 1;BN6PR01MB2449;7:04iNxAVf4CQ0l81i63BUh86PGeAiHsSRrWhAUHYb8EXkwjsxDOIepXmKd7dlPFqEIkWAPPQurfKWBs4oFh/j2gk1TLwBivny8SP80X97Ow8HN5Ta8jejnhPxH2ql3wRl0LSSIPKZPSCo09+jxeqeist6N+0NPiTzrhH9+qOxE1bbaGjHQOYOwYqdKBTfZUGmdcy5i3jU7Cf5Q0HOMyLDXkIdsNVlZL6+2/bYuA5ymdfzX9KIsU/adRLngB4++s6G/Fcyr/8xO0gJOM3z8tNtedBCrDIKwZti1qU96wsv+KEDalls6hpyOmtf1J8NktKvSOvrOzkPmhx3+vlOU6jU8w== 6, 48 -- x-ms-traffictypediagnostic: BN6PR01MB2449: 6, 48 -- x-ms-office365-filtering-correlation-id: fa554be2-cbb8-429f-7591-08d4ac61253f 6, 48 -- x-microsoft-antispam: UriScan:;BCL:0;PCL:0;RULEID:(22001)(2017030254075)(201703131423075)(201703031133081)(201702281549075);SRVR:BN6PR01MB2449; 6, 48 -- x-microsoft-antispam-prvs: {BN6PR01MB2449A8189533EF39CFBB766BBFCA0-at-BN6PR01MB2449.prod.exchangelabs.com} 6, 48 -- x-exchange-antispam-report-test: UriScan:; 6, 48 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:(100000700101)(100105000095)(100000701101)(100105300095)(100000702101)(100105100095)(6040450)(2401047)(8121501046)(5005006)(93006095)(93001095)(3002001)(10201501046)(100000703101)(100105400095)(6041248)(20161123564025)(20161123555025)(20161123562025)(20161123558100)(20161123560025)(201703131423075)(201702281529075)(201702281528075)(201703061421075)(201703061406153)(6072148)(100000704101)(100105200095)(100000705101)(100105500095);SRVR:BN6PR01MB2449;BCL:0;PCL:0;RULEID:(100000800101)(100110000095)(100000801101)(100110300095)(100000802101)(100110100095)(100000803101)(100110400095)(100000804101)(100110200095)(100000805101)(100110500095);SRVR:BN6PR01MB2449; 6, 48 -- x-forefront-prvs: 0329B15C8A 6, 48 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10009020)(6009001)(39410400002)(39850400002)(39400400002)(39450400003)(39840400002)(39860400002)(2351001)(3660700001)(6436002)(110136004)(6916009)(38730400002)(122556002)(72206003)(99286003)(75432002)(305945005)(74316002)(7736002)(14454004)(53936002)(478600001)(7696004)(9686003)(55016002)(189998001)(5660300001)(86362001)(5640700003)(2906002)(3280700002)(8936002)(33656002)(102836003)(2900100001)(25786009)(42882006)(88552002)(77096006)(54356999)(6506006)(8676002)(81166006)(6116002)(50986999);DIR:OUT;SFP:1101;SCL:1;SRVR:BN6PR01MB2449;H:BN6PR01MB2449.prod.exchangelabs.com;FPR:;SPF:None;MLV:sfv;LANG:en; 6, 48 -- spamdiagnosticoutput: 1:99 6, 48 -- spamdiagnosticmetadata: NSPM 6, 48 -- Content-Type: text/plain; charset="iso-8859-1" 6, 48 -- MIME-Version: 1.0 6, 48 -- X-OriginatorOrg: rockets.utoledo.edu 6, 48 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 05 Jun 2017 22:21:00.0982 6, 48 -- (UTC) 6, 48 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted 6, 48 -- X-MS-Exchange-CrossTenant-id: 1d6b1707-baa9-4a3d-a8f8-deabfb3d467b 6, 48 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: BN6PR01MB2449 6, 48 -- Content-Transfer-Encoding: 8bit 6, 48 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v55M7ot1006755 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both tagided-at-umbc.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Ultracut E or S control box
Message: I'm looking for both a Reichert Ultracut E and S control box. If anyone has a box or would know where I could find one to purchase, please let me know. Thanks! Tagide deCarvalho
Login Host: 130.85.116.160 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- ============================================= Do no rely to this messaage it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both lavoie-at-uw.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: lavoie-at-uw.edu Name: Ellen Lavoie
Organization: UW - MAF
Title-Subject: [Filtered] FEI single tilt holder
Message: Hello all,
I'm trying to narrow down some odd contamination on our FEI ST holder but am posing the the following question:
Exactly what elements should show up in a case of a pristine new ST holder? Obviously, yes, I know there is naturally some contamination and variation with wear and age. Have not contacted tech at FEI yet because I thought surely someone out here in microscopy land has tested this??
Cheers, Ellen Login Host: 128.95.174.10 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- ============================================= Do no rely to this messaage it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
I guess you are referring to the Ethanolic Phosphotungstic Acid / Ethanolic PTA (E-PTA) staining technique which was introduced by Gray (1959) [cf: Stains and Cytochemical Methods, M.A. Hayat(ed) 1993] Ethanolic Phosphotungstic Acid, Chapter 8, p 284, where you can find a collection of stained and unstained material/tissue components, predominantly of/regarding nerve synapse(s)
Perhaps you can find some or more of the information you are looking for: in: Assembly of Proteins to Postsynaptic Densities after Transient Cerebral Ischemia Bing-Ren Hu, Minkyu Park, Maryann E. Martone, Wolfgang H. Fischer, Mark H. Ellisman, and Justin A. Zivin In: J. Neurosci., January 15, 1998, 18(2):625633 X-from: Materials and Methods Electron microscopic studies Tissue sections from experimental and control animals were stained with 1% ethanolic phosphotungstic acid (E-PTA) by the method of Bloom and Aghajanian (1966, 1968). Coronal brain sections were cut to a thickness of 200 mm with a Vibratome through the level of the dorsal hippocampus and post-fixed for 1 hr with 4% glutaraldehyde in 0.1 M cacodylate buffer, pH 7.4. Sections then were dehydrated in an ascending series of ethanol to 100% and stained for 1 hr with 1% phosphotungstic acid prepared by dissolving 0.1 gm of PTA in 10 ml of 100 ethanol and then adding four drops of 95% ethanol from a Pasteur pipette. Sections were embedded in Durcopan ACM. Isolated PSDs were fixed with 4% glutaraldehyde in 0.1 M cacodylate buffer, pH 7.4, osmicated for 60 min in 1% osmium tetroxide, stained en bloc in 1% aqueous uranyl acetate, dehydrated, and embedded in Durcopan ACM. Ultrathin and semithin (1 mm) sections of parietal cortex (layers IIV) were cut and evaluated with a JEOL 100CX electron microscope or a JEOL 4000EX intermediate high-voltage electron microscope without additional staining. Sections of isolated PSDs were counterstained with lead citrate before examination in the electron microscope. [not knowing whether your methodological approach is similar to the described one in the study]: "The E-PTA method selectively stains the postsynaptic density, the presynaptic grid, and material in the synaptic cleft but leaves most other structures less stained (Bloom and Aghajanian, 1966, 1968)." X-from References: Bloom FE, Aghajanian GK (1966) Cytochemistry of synapses: a selective staining method for electron microscopy. Science 154:15751577. Bloom FE, Aghajanian GK (1968) Fine structural and cytochemical analysis of staining of synaptic junctions with phosphotungstic acid. J Ultrastruct Res 22:361375.
Also, you can find hints on the nature of material which is stained (or less or not stained) by E-PTA in: R A Horowitz and C L Woodcock: Alternative staining methods for Lowicryl sections J Histochem Cytochem 1992 40: 123; DOI: 10.1177/40.1.1370308, -at-http://jhc.sagepub.com/content/40/1/123
Perhaps a further reading: OBSERVATIONS ON THE KINETICS OF URANYL ACETATE AND PHOSPHOTUNGSTIC ACID STAINING OF CHROMATIN IN THIN SECTIONS FOR ELECTRON MICROSCOPY P. A. CATTINI and H. G. DAVIES STAIN TECHNOLOGY Vol. 59. No. 5 pp291-304 Copyright(C) 1984 by The Williams & Wilkins Co.
Many articles more can be found Googleing for | e-PTA OR "ethanolic PTA" specific* staining | Googleing | e-PTA OR "ethanolic PTA" binding specific* staining | reduces the number of results and finds some rapid info about: { Ethanolic phosphotungstic acid staining of glutaraldehyde fixed cells specifically contrasts basic proteins, and reveals structural details that are not easily discerned in uranyl acetate stained or osmicated sections (Bloom and Aghajanian, 1966; 1968). .... procyclic T. brucei cell stained in this manner. The nucleus and kinetoplast are strongly contrasted and the cytoplasm shows a granular structure of intermediate contrast. The mitochondrial matrix is not contrasted. ...... Strong binding of E-PTA to basic residues of histone proteins (Sheridan and Barrnett, 1969) accounts for the strong contrast observed in the nucleus. Lysine and arginine residues of proteins are believed to be the binding sites for the PTA, a suggestion supported by the finding that acetylation reduced PTA binding to isolated histones (Sheridan and Barrnett, 1969). Cited from: Structural asymmetry and discrete nucleic acid subdomains in the Trypanosoma brucei kinetoplast GLUENZ et al, 2007: Mol Microbiol. 2007 Jun 1; 64(6): 15291539. doi: 10.1111/j.1365-2958.2007.05749.x; PMCID: PMC1974780; cf. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1974780/
Hope this helps anyway in a first attempt,
Best wishes and regards Wolfgang MUSS, PhD Retired MSA-Member SALZBURG, AUSTRIA
-----Ursprngliche Nachricht----- Von: Kyoung.Jo-at-rockets.utoledo.edu [mailto:Kyoung.Jo-at-rockets.utoledo.edu] Gesendet: Dienstag, 6. Juni 2017 00:28 An: wij.muss-at-aon.at Betreff: [Microscopy] TEM need help on staining methods ---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 6, 48 -- From Kyoung.Jo-at-rockets.utoledo.edu Mon Jun 5 17:07:51 2017 6, 48 -- Received: from NAM03-CO1-obe.outbound.protection.outlook.com (mail-co1nam03on0056.outbound.protection.outlook.com [104.47.40.56]) 6, 48 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v55M7ot1006755 6, 48 -- for {Microscopy-at-microscopy.com} ; Mon, 5 Jun 2017 17:07:51 -0500 6, 48 -- Received: from BN6PR01MB2449.prod.exchangelabs.com (10.173.139.147) by 6, 48 -- BN6PR01MB2449.prod.exchangelabs.com (10.173.139.147) with
I'll assume most of the noise in an image of an ice-embedded bio-molecule is due to variations in the ice, so called structural noise. The CTF describes the contrast transfer for both signal and noise in the same way. So, what is the point of changing the CTF by defocusing and/or using a phase plate. The SNR should be unchanged.
I can think of one explanation: If the signal spectrum is very different from the noise spectrum one could chose a CTF that enhances the resolution bands where the difference is big.
Else: Is there some other factor that affects visibility of the molecule than SNR?
All the best,
Philip
==============================Original Headers============================== 8, 23 -- From Philip.Koeck-at-ki.se Wed Jun 7 03:11:32 2017 8, 23 -- Received: from KIMSX10.user.ki.se (kimsx10.user.ki.se [130.229.20.27]) 8, 23 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v578BWgV004179 8, 23 -- for {microscopy-at-microscopy.com} ; Wed, 7 Jun 2017 03:11:32 -0500 8, 23 -- Received: from KIMSX03.user.ki.se ([fe80::3046:45c2:90af:a23]) by 8, 23 -- KIMSX10.user.ki.se ([fe80::c823:84e7:ce2b:9967%11]) with mapi id 8, 23 -- 14.03.0248.002; Wed, 7 Jun 2017 10:24:45 +0200 8, 23 -- From: Philip Koeck {Philip.Koeck-at-ki.se} 8, 23 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 8, 23 -- Subject: [TEM] CTF and SNR 8, 23 -- Thread-Topic: [TEM] CTF and SNR 8, 23 -- Thread-Index: AdLfZfPNGa9HTVUfSmGuOYeCI6H0wAAAXn3Q 8, 23 -- Date: Wed, 7 Jun 2017 08:24:45 +0000 8, 23 -- Message-ID: {C8D24D7E19AEE543942A826EE910B1C201810440C3-at-KIMSX03.user.ki.se} 8, 23 -- Accept-Language: en-GB, sv-SE, en-US 8, 23 -- Content-Language: sv-SE 8, 23 -- X-MS-Has-Attach: 8, 23 -- X-MS-TNEF-Correlator: 8, 23 -- x-originating-ip: [130.229.20.43] 8, 23 -- Content-Type: text/plain; charset="us-ascii" 8, 23 -- MIME-Version: 1.0 8, 23 -- Content-Transfer-Encoding: 8bit 8, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v578BWgV004179 ==============================End of - Headers==============================
Dear All, there are news from Wuerzburg and the nanoflight system: I finally bought a very nice new FE-SEM, a MIRA3 from TESCAN.
Have a look at some trials:
T-Cells: https://vimeo.com/216668452
This is the first extra-small field-of-view test of my new MIRA3 FE-SEM on some T-cells supplied by Bruce Levine, Purdue University. The single cell is ca. 12 micron wide, the round iron beads five micron. In my opinion this is the first complete 360 curve ever "flown" actually around a T-cell in the scanning electron microscope. Field-of-view at the end of the second sequence is ca. 45 micron wide. These sequences had been a first test for the accuracy of both the SEM electronics and the SmarAct 8 axes piezostage. Both came out very good.
Think about the accuracy needed to succeed twice: first more than 30 waypoints for the sequences are set up, then overlooked at a "pre-flight", then going back to the beginning and starting sending out the interpolated frame positions, more than 500... I ended up in a voxel of ca. 5x5x5 micron inaccuracy.
Here is a second nanoflight on a very small 3d printed elephant (150x150x80 micron size): https://vimeo.com/216811056
I hope that in a few weeks I will be able to do 3D nanoflights in SEM down to cell size.
I am looking for interesting specimen - preferably single cells on glass - to be imaged as nanoflights or to be reconstructed as 3d models with SFM software.
Anybody willing to join the project: feel free to contact me.
Best wishes, Stefan
--
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
==============================Original Headers============================== 19, 22 -- From stefan.diller-at-t-online.de Wed Jun 7 04:47:00 2017 19, 22 -- Received: from mailout04.t-online.de (mailout04.t-online.de [194.25.134.18]) 19, 22 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v579kx7E028295 19, 22 -- for {microscopy-at-microscopy.com} ; Wed, 7 Jun 2017 04:47:00 -0500 19, 22 -- Received: from fwd27.aul.t-online.de (fwd27.aul.t-online.de [172.20.26.132]) 19, 22 -- by mailout04.t-online.de (Postfix) with SMTP id 6EFEF41B9477 19, 22 -- for {microscopy-at-microscopy.com} ; Wed, 7 Jun 2017 12:00:14 +0200 (CEST) 19, 22 -- Received: from mac-pro.local (X7Yo-iZSghijZhuMRPhw5b0A3USntENay+Y-SMsY9PcJpkLfCM1xeA7dvqaC7JBgFn-at-[80.130.172.167]) by fwd27.t-online.de 19, 22 -- with (TLSv1.2:ECDHE-RSA-AES256-SHA encrypted) 19, 22 -- esmtp id 1dIXl3-1r17JY0; Wed, 7 Jun 2017 12:00:09 +0200 19, 22 -- To: "Microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 19, 22 -- From: Stefan Diller {stefan.diller-at-t-online.de} 19, 22 -- Subject: High-end projects in SEM ? 19, 22 -- Message-ID: {e3c6ef85-0859-f239-bd12-ee4bdeca71ee-at-t-online.de} 19, 22 -- Date: Wed, 7 Jun 2017 12:00:08 +0200 19, 22 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:45.0) 19, 22 -- Gecko/20100101 Thunderbird/45.8.0 19, 22 -- MIME-Version: 1.0 19, 22 -- Content-Type: text/plain; charset=windows-1252; format=flowed 19, 22 -- Content-Transfer-Encoding: 7bit 19, 22 -- X-ID: X7Yo-iZSghijZhuMRPhw5b0A3USntENay+Y-SMsY9PcJpkLfCM1xeA7dvqaC7JBgFn 19, 22 -- X-TOI-MSGID: a5b47788-6012-4218-bd09-5796d9707c47 ==============================End of - Headers==============================
From johnsonloretta061-at-gmail.com Wed Jun 7 10:06:59 2017 Return-Path: {johnsonloretta061-at-gmail.com} Received: from gmail.com ([182.16.8.146]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v57F6vo1024695 for {microscopylistserverarchive6-at-microscopy.com} ; Wed, 7 Jun 2017 10:06:58 -0500 Message-ID: {F323A353.354842AF-at-gmail.com}
X-from: mryan-at-cshl.edu
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mryan-at-cshl.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: mryan-at-cshl.edu Name: Marjorie Ryan
Organization: Cold Spring Harbor Laboratory
Title-Subject: [Filtered] Microscopy Research Associate position.
Message: Thank you very much for your assistance.
Sincerely,
Marjorie Ryan Cold Spring Harbor Laboratory mryan-at-cshl.edu
Research Associate Electron Microscopy Technologist
Cold Spring Harbor Laboratory seeks a highly motivated dedicated individual to work in a state-of-the-art Microscopy Shared Resource.
The individual should have extensive practical expertise in biological sample preparation for transmission and scanning electron microscopy. Hands-on knowledge of confocal and widefield fluorescence microscopy would also be a plus. The candidate will help users design innovative experiments and they will carry out sample preparation and imaging as well as assist in data interpretation.
Excellent verbal and written communication skills, ability to work with multiple users in a supporting role, and ability to work independently and proactively with limited supervision are essential. A Bachelors degree in biology or related discipline is required. One to three years of experience working in a Microscopy Shared Resource is preferred.
How to Apply:
Email: mryan-at-cshl.edu Interested individuals should also apply for this position via the CSHL careers website at https://cshl.peopleadmin.com/postings/11688
Position Number 01779-R
Applicants should include a resume along with a description of their practical expertise and the names as well as email addresses of 3 references.
Cold Spring Harbor Laboratory is a world-renowned research and educational institution recognized internationally for its excellence in ground-breaking research programs in cancer, neuroscience, plant biology, genomics, and bioinformatics and broad educational mission.
For more information about CSHL, please visit us at www.cshl.edu
CSHL is an EO/AA Employer. All qualified applicants will receive consideration for employment and will not be discriminated against on the basis of race, color, religion, sex, sexual orientation, gender identity, national origin, age, disability or protected veteran status.
VEVRAA Federal Contractor
Login Host: 143.48.14.89 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both stacie-at-ems-secure.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Message: EMS Microscopy Academy announces our X-Ray Microanalysis Workshop: A Complete Picture. This course covers qualitative and semi quantitative analysis beginning with the generation of background and characteristic of x-rays, nomenclature, and peak family ratios.
September 13 - 15, 2017 8:00 a.m. - 4:30 p.m. Hatfield, Pennsylvania, USA
For more information, visit http://ow.ly/Zb9F30cmyuf
Login Host: 73.165.127.32 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both kmicheva-at-stanford.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Organization: Stanford University School of Medicine
Title-Subject: [Filtered] Job opening at Stanford: Life Science Technician II
Message: Job Description Life Science Technician II - 75238 Description The Department of Molecular and Cellular Physiology is seeking a Life Science Technician II to assist with preparation of laboratory biological tissue specimens for Array Tomography, and for general laboratory maintenance and organization.
Duties include preparation of specimens and reagents for immunostaining, light and electron microscopy, tissue thin and ultra-thin sectioning, handling of experimental animals (rats and mice), including whisker trimming and perfusion, fluorescent image acquisition, and analysis of volumetric image data, basic statistical analysis, experimental record-keeping, general laboratory compliance and record-keeping tasks, and general laboratory maintenance and organization.
Applicants already having experience in desired skills are preferred, but training in specific procedures will be provided. Qualifications Experience in the skills/duties listed above is preferred, but aptitude is required and training will be provided. Training, if necessary, in general laboratory tasks will be provided.
In addition: 2+ year of relevant laboratory experience or relevant college coursework. A strong interest in cellular neuroscience. Meticulous attention to detail and record keeping in laboratory procedures. Excellent manual dexterity. Preferred: Experience working in a biology laboratory. Preferred: Ultrathin sectioning. Preferred: Understanding of basic principles of light microscopy. Preferred: Experience with animal handling (rodents) and perfusion fixation. Ability to work independently and within a collaborative environment. Proficiency with Microsoft Office software (Word, Excel, PowerPoint). Aptitude for learning and use of scientific software (ImageJ). Job : Research Location : School of Medicine Schedule : Full-time Grade: A24 Job Code: 5644
Please apply at http://stanfordcareers.stanford.edu/job-search Job #75238
Login Host: 171.66.212.131 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
We are pleased to announce that the Electron Microscopy Core Imaging Facility (EMCIF) at the University of Maryland Baltimore will be offering a Biological EM Sample Processing mini-course on July 13th and 14th, 2017 http://www.dental.umaryland.edu/UMBEMProcessingCourse/ .
The course is designed to teach any individual who wishes to learn biological sample processing for electron microscopy. No experience is required. The course will be limited to six participants.
--Course format: lectures, demonstrations, and hands-on practice.
--Topics: Chemical fixation, cryo-immobilization, resin embedding, conventional and modern rapid processing methods, microwave assisted processing, automated sample processing, SEM biological sample processing and Negative staining of particulate specimen.
--Instrument Demonstration: high pressure freezer, automated freeze substitution, critical point dryer and automated EM sample processing
--Other related courses: EMCIF also offers room temperature ultramicrotomy and immunogold labeling mini courses in September and October, this year. The minicourses follow a similar format covering principles, practices, and troubleshooting with an emphasis on hands-on practice and instrument demonstration.
--More information: Email coreimaging-at-umaryland.edu or visit our website http://www.dental.umaryland.edu/core-imaging/workshops-and-courses/
Thanks.
Sincerely,
Ru-ching Hsia, Ph.D. Associate Professor and Director Electron Microscopy Core Imaging Facility University of Maryland, Baltimore Rm 696B, Howard Hall, 660 W. Redwood St. Baltimore, MD 21201
==============================Original Headers============================== 11, 59 -- From hsia627-at-hotmail.com Tue Jun 13 19:01:10 2017 11, 59 -- Received: from NAM01-BN3-obe.outbound.protection.outlook.com (mail-oln040092000109.outbound.protection.outlook.com [40.92.0.109]) 11, 59 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v5E01AV2014057 11, 59 -- for {microscopy-at-microscopy.com} ; Tue, 13 Jun 2017 19:01:10 -0500 11, 59 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=hotmail.com; 11, 59 -- s=selector1; h=From:Date:Subject:Message-ID:Content-Type:MIME-Version; 11, 59 -- bh=G6xsGEHmbdO3eX0erKN8dGBsquHcnBEBFh+khSFmEIA=; 11, 59 -- b=ehC5y+TDelNVFMWQ06VK8tWe4lLt3Nlm7F1Tnof2B6Fh6DR4/xjZS7159LGohdU+WLkYO3E50Rtl9pAodRq5P9LUiS6yvjaKxXVqX/ToWvw4fq6NsmLV/2rjpDuiV7leTRkXQRxGG3NxH/dAqXc1HmUVtUovtCRJMZhSPl27ajgAOgi6nGkVnu5K54q36zqHSZoFi4W7hrYQprV2vD2ano0mGvtCm+6hf2QkPpZZyud80VU4Uslxjvslq8EPQM1yVxGvm4ThbHOTi3SXXuqTQOqNRlxUzUbVZkjx/p1G4tsRS/3xBSfk7TcS0hV70IqkLG2MBNyqcN0mhVB+cKs7zA== 11, 59 -- Received: from SN1NAM01FT023.eop-nam01.prod.protection.outlook.com 11, 59 -- (10.152.64.59) by SN1NAM01HT197.eop-nam01.prod.protection.outlook.com 11, 59 -- (10.152.65.49) with Microsoft SMTP Server (version=TLS1_2, 11, 59 -- cipher=TLS_ECDHE_RSA_WITH_AES_256_CBC_SHA384_P384) id 15.1.1143.11; Wed, 14 11, 59 -- Jun 2017 00:14:49 +0000 11, 59 -- Received: from BLUPR04MB660.namprd04.prod.outlook.com (10.152.64.60) by 11, 59 -- SN1NAM01FT023.mail.protection.outlook.com (10.152.64.168) with Microsoft SMTP 11, 59 -- Server (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_128_CBC_SHA256_P256) id 11, 59 -- 15.1.1157.12 via Frontend Transport; Wed, 14 Jun 2017 00:14:49 +0000 11, 59 -- Received: from BLUPR04MB660.namprd04.prod.outlook.com ([10.141.204.155]) by 11, 59 -- BLUPR04MB660.namprd04.prod.outlook.com ([10.141.204.155]) with mapi id 11, 59 -- 15.01.1157.012; Wed, 14 Jun 2017 00:14:50 +0000 11, 59 -- From: Ruching hsia {hsia627-at-hotmail.com} 11, 59 -- To: "Microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 11, 59 -- Subject: Course announcement: Biological EM Sample Processing 07/13-14, 2017 11, 59 -- Thread-Topic: Course announcement: Biological EM Sample Processing 07/13-14, 11, 59 -- 2017 11, 59 -- Thread-Index: AQHS5KMHM2yXRICSlk6PzxzUbzoLlw== 11, 59 -- Date: Wed, 14 Jun 2017 00:14:50 +0000 11, 59 -- Message-ID: {BLUPR04MB6601452AE24298E6CB94AD4EFC30-at-BLUPR04MB660.namprd04.prod.outlook.com} 11, 59 -- Accept-Language: en-US 11, 59 -- Content-Language: en-US 11, 59 -- X-MS-Has-Attach: 11, 59 -- X-MS-TNEF-Correlator: 11, 59 -- authentication-results: microscopy.com; dkim=none (message not signed) 11, 59 -- header.d=none;microscopy.com; dmarc=none action=none header.from=hotmail.com; 11, 59 -- x-incomingtopheadermarker: OriginalChecksum:69B7E9FAC03931DC90BF5967C9566D493A2767162CFA572F94CC9569DFFF1434;UpperCasedChecksum:B165499130BB4C0F8B7CAC96A3259B434CB3FDC699B6B6A112440B38ABB502E1;SizeAsReceived:7104;Count:43 11, 59 -- x-ms-exchange-messagesentrepresentingtype: 1 11, 59 -- x-tmn: [lFYiXgYPY4+UIEIaI5o7/1LNmZwswwVk] 11, 59 -- x-ms-publictraffictype: Email 11, 59 -- x-microsoft-exchange-diagnostics: 1;SN1NAM01HT197;24:XVFpLQ4/3VmEo3N5qhiWVxSLXJg+DMM0QiDI9plggcPvXrpJl/zv2EInMxRQCx/4HUcuHDfyFHuntit+G09D+GRy7HoDb7qEIX5GivFiDU0=;7:Wkrqcgpkm8kooNv8/sY8uZcPJ8JnIKY5XCOmwqrX7ik1tyAFlCFN3qjIhpzVWfO8B9msQjZ8P67/1llmYmTlIiGax/8bhJaC/U1LSM+rYs8G1UCak9LB1f03G/aTKRXmCNX0cSFw2kfpmG9cGeh6AGqIjMMOlfp8UN23RVakSH2XY3hboFkyxm70F6KcUdLicnj45fBgMFzXH1TOKwijCfmaopej9RiD4AV9vAY2inbeQmGiOhm8Y3I5+WkLBoF59gHy8KS9LnxUuT4SN9w+jkFFgClpCMusbD7xqDuN/hA6yMuXjcfnVQ3rl154Kg9u 11, 59 -- x-incomingheadercount: 43 11, 59 -- x-eopattributedmessage: 0 11, 59 -- x-forefront-antispam-report: EFV:NLI;SFV:NSPM;SFS:(7070007)(98901004);DIR:OUT;SFP:1901;SCL:1;SRVR:SN1NAM01HT197;H:BLUPR04MB660.namprd04.prod.outlook.com;FPR:;SPF:None;LANG:en; 11, 59 -- x-ms-traffictypediagnostic: SN1NAM01HT197: 11, 59 -- x-ms-office365-filtering-correlation-id: 51484003-25e3-42be-f2ca-08d4b2ba5f76 11, 59 -- x-microsoft-antispam: UriScan:;BCL:0;PCL:0;RULEID:(22001)(201702061074)(5061506573)(5061507331)(1603103135)(2017031320274)(2017031324274)(2017031323274)(2017031322274)(1601125374)(1603101448)(1701031045);SRVR:SN1NAM01HT197; 11, 59 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:(100000700101)(100105000095)(100000701101)(100105300095)(100000702101)(100105100095)(444000031);SRVR:SN1NAM01HT197;BCL:0;PCL:0;RULEID:(100000800101)(100110000095)(100000801101)(100110300095)(100000802101)(100110100095)(100000803101)(100110400095)(100000804101)(100110200095)(100000805101)(100110500095);SRVR:SN1NAM01HT197; 11, 59 -- x-forefront-prvs: 033857D0BD 11, 59 -- spamdiagnosticoutput: 1:99 11, 59 -- spamdiagnosticmetadata: NSPM 11, 59 -- Content-Type: text/plain; charset="iso-8859-1" 11, 59 -- MIME-Version: 1.0 11, 59 -- X-OriginatorOrg: hotmail.com 11, 59 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 14 Jun 2017 00:14:50.0123 11, 59 -- (UTC) 11, 59 -- X-MS-Exchange-CrossTenant-fromentityheader: Internet 11, 59 -- X-MS-Exchange-CrossTenant-id: 84df9e7f-e9f6-40af-b435-aaaaaaaaaaaa 11, 59 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: SN1NAM01HT197 11, 59 -- Content-Transfer-Encoding: 8bit 11, 59 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v5E01AV2014057 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both eduardo.isoppo-at-ufsc.br as well as the Microscopy Listserver ---------------------------------------------------------------------------
Organization: Federal University of Santa Catarina
Title-Subject: [Filtered] TEM - Camera-head eletronics for Gatan model 794
Message: Hello All, My name is Eduardo and Im working in a electron microscopy facility in Florianopolis - Brazil. Our CCD camera (Gatan 794) installed in the TEM (Jeol 2100) is down due problems in the camera-head eletronics. The circuit has to be replaced. Gatan (By Jeol - Brazil) said that this model is no longer available for maintenance service (it is too old). Does anyone here knows where I can get support for this camera or where I can find parts for this camera? thanks
-- Eduardo de Almeida Isoppo, Ph.D Physicist Federal University of Santa Catarina - UFSC Florianpolis/SC - Brazil
Login Host: 150.162.111.28 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both apakzad-at-gatan.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: apakzad-at-gatan.com Name: Ana Pakzad
Organization: Gatan Inc.
Title-Subject: [Filtered] Job Opening: Summer Internship at Gatan Message: We are seeking candidates for a summer internship position at Gatan, CA office. This role will support our R&D team by conducting electron microscopy on various types of materials, analysis of results and preparing reports. For more details please go to: http://www.gatan.com/company/careers/summer-internship-imaging
Login Host: 50.58.128.11 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both vitha-at-tamu.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: vitha-at-tamu.edu Name: Stanislav Vitha
Organization: Texas A&M University
Title-Subject: [Filtered] TEM/Cryo-TEM: Research Scientist position available
Message: The Microscopy and Imaging Center (MIC) at Texas A&M University is seeking to hire a staff research scientist. The new hire will provide service and user training in TEM/cryo-TEM imaging, sample preparation, and in situ elemental/molecular analyses of biological and soft matter material science samples. Required Education and Experience: A doctoral degree in a life science related field plus six years of experience in electron microscopy.
For additional details, please see http://microscopy.tamu.edu/whats-new Instructions for Applicants: Please submit a resume/CV and cover letter to joeashworth-at-tamu edu to apply.
Login Host: 128.194.151.93 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
Hi We are trying to pull out the stage of our JSM5600LV machine and separate it completely from the chamber. Can anybody help us to tell what screws we have to remove? Thanks in advance for your time yorgos
Does anyone have experience with post-fixing Tokuyasu cryosections with osmium tetroxide? The purpose would be to gain better and more “standard” membrane contrast. I tried to fix the grids on a drop of 2% OsO4, but there was very little (if any) change. I am sure people tried before, but do not remember reading about it. Maybe OTO enhancement or...?
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both lbochtler-at-protonmail.ch as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: lbochtler-at-protonmail.ch Name: Lukas B.
Title-Subject: [Filtered] Hitachi S800 Manual and Service manual Request
Message: Hello,
I just scored a Hitachi S800 electron microscope, sadly most cables between the main console (with the electron column) and the control desk have been cut. I can replace most if not all cables, so that isn't much of a problem.
However, i did not get any documentation with it, since i don't know if the original owner will ever manage to find any documentation for it, i decided to ask you guys.
Ideally i would need both the user and service manual for the microscope, possibly with the computer software (since im not sure if i can get the computer for it as well, since the microscope and the other parts are now in 2 different locations)
Ideally id also need the power requirements for the microscope, what voltage dose it need, how much power dose it draw, and how many power phases dose it need.
Best regards,
Lukas B.
Login Host: 62.245.208.5 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both M.Simonelli-at-nottingham.ac.uk as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: M.Simonelli-at-nottingham.ac.uk Name: Marco Simonelli
Organization: University of Nottingham
Title-Subject: [Filtered] light elements measurement in metals
Message: Dear Fellow Microscopists,
I am looking for an advice on a reliable technique to measure the concentration of light elements (an in particular C) in metal/alloys. I would be very grateful if you can share with me your experience and comments.
Many thanks, Regards, Marco
Dr Marco Simonelli Centre for Additive Manufacturing The University of Nottingham University Park Nottingham, UK
Login Host: 128.243.2.29 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
I just wondering about nobody seems to have answered to your interesting question until now (but it could be that you, dear Michal, have gotten some personal replies due to so many unnecessary OOO-[NOT ‚OTO‘- (☺) ]messages from the list itself).
If the latter (some personal Re’s) is fact, would you mind to post the result(s) or a summary of personally received answers, if the former is true, it is to regret that obviously nobody has an answer or it is no more interesting.
As of my "memories" [legacy of ancillary knowledge] searching the web it might be there are solutions but prior to be a „ know it all“ I would like to ask you, dear Michal, whether your problem with enhancing the contrast in your cryosections (if possible: which tissue / specimens - biological, materials?, polymers? How your cryosections are collected from the knife edge [i.e. dry, wet by allowing to thaw on the grid] ? ) persists or / and what – if ever – answers to your original questions were. For using OTO-method it is not too late but perhaps there are thinkable methods you should try in the first place....
Thanking in advance for your understanding, best wishes and Season's Greetings
sincerely yours,
MUSS Wolfgang PhD (Dr. phil.) [OR i. R. / en retraite / private / retired] FRMS, MSA-member, Emeritus status Ignaz-Rieder-Kai 19/6 A-5020 SALZBURG Österreich-AUSTRIA Mobil-Tel.: 0043(0)676 5 369 456 E-mail: wij.muss-at-aon.at E-Mail altern.: womuss-at-gmail.com
==================================================================================== On Mi 14.06.2017 21:23 Michal Jarnik / NIH { michal.jarnik-at-nih.gov} wrote: ---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Dear listers,
Does anyone have experience with post-fixing Tokuyasu cryosections with osmium tetroxide? The purpose would be to gain better and more “standard†membrane contrast. I tried to fix the grids on a drop of 2% OsO4, but there was very little (if any) change. I am sure people tried before, but do not remember reading about it. Maybe OTO enhancement or...?
Thanks for ideas,
Michal Jarnik NIH ====================================================================================
==============================Original Headers============================== 23, 21 -- From wij.muss-at-aon.at Mon Jun 19 06:49:47 2017 23, 21 -- Received: from bsmtp7.bon.at (bsmtp7.bon.at [213.33.87.19]) 23, 21 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v5JBnlWh000646 23, 21 -- for {microscopy-at-microscopy.com} ; Mon, 19 Jun 2017 06:49:47 -0500 23, 21 -- Received: from MussTHINK (unknown [93.83.25.98]) 23, 21 -- by bsmtp7.bon.at (Postfix) with ESMTPSA id 3wrqPv2Js6z5tlV; 23, 21 -- Mon, 19 Jun 2017 14:03:47 +0200 (CEST) 23, 21 -- From: "MUSS Wolfgang Dr. phil./PhD \(OR i.R,retired\)" {wij.muss-at-aon.at} 23, 21 -- To: {microscopy-at-microscopy.com} 23, 21 -- Cc: {michal.jarnik-at-nih.gov} 23, 21 -- Subject: just to be sure about replies from MSA-Listserver....and Season's Greetings / good wishes for the summertime 23, 21 -- Date: Mon, 19 Jun 2017 14:03:48 +0200 23, 21 -- Message-ID: {000001d2e8f4$1c813620$5583a260$-at-aon.at} 23, 21 -- MIME-Version: 1.0 23, 21 -- Content-Type: text/plain; 23, 21 -- charset="utf-8" 23, 21 -- X-Mailer: Microsoft Outlook 15.0 23, 21 -- Thread-Index: AdLo8Lyk8Sz2YaLLREWHjhXt6OSePg== 23, 21 -- Content-Language: de 23, 21 -- Content-Transfer-Encoding: 8bit 23, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v5JBnlWh000646 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mgriffit-at-lakeheadu.ca as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: mgriffit-at-lakeheadu.ca Name: Martin Griffith
Organization: Lakehead University
Title-Subject: [Filtered] E.F. Fullam sputter coater head
Message: Does anyone know how I can procure a sputter coater head for an Ernest F. Fullam EFFA sputter coater?
Login Host: 216.211.111.216 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- ============================================= Do no rely to this messaage it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mgriffit-at-lakeheadu.ca as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: mgriffit-at-lakeheadu.ca Name: Martin Griffith
Organization: Lakehead University
Title-Subject: [Filtered] E.F. Fullam sputter coater head
Message: Does anyone know how I can procure a sputter coater head for an Ernest F. Fullam EFFA sputter coater?
Login Host: 216.211.111.216 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- ============================================= Do no rely to this messaage it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mgriffit-at-lakeheadu.ca as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: mgriffit-at-lakeheadu.ca Name: Martin Griffith
Organization: Lakehead University
Title-Subject: [Filtered] E.F. Fullam sputter coater head
Message: Does anyone know how I can procure a sputter coater head for an Ernest F. Fullam EFFA sputter coater?
Login Host: 216.211.111.216 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- ============================================= Do no rely to this messaage it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
The early registration deadline for M&M 2017 is just a few days away and as you are making plans to attend, please don't forget about MSA's student bursary program. Its purpose is to encourage students to attend the meetings by helping to defray some of the cost and giving them an opportunity to meet and interact with the established microscopy community.
The students will be paid $10 an hour to work for ~15-20 hours during the meeting or pre-meeting events (paid by check at end of meetings). The jobs involve providing support in the different symposia, staffing the volunteer office; newsletter distribution, or helping with vendor tutorial sign-up. There is an added bonus of a meeting t-shirt and $10 cash for each morning and/or afternoon session worked to assist with meals.
Volunteers are also needed to help with the above mentioned meeting tasks since not all will be filled by bursaries. Although not paid the hourly rate as the students, volunteers are given the same $10 cash to assist with meals and meeting t-shirt. They also have the opportunity to interact more with the microscopy community while assisting with the meeting.
If anyone would like to participate in the bursary program, would be willing to volunteer, or has any questions please contact Amanda Lawrence (alawrence-at-i2at.msstate.edu).
Don't forget the early registration deadline is June 26. Meetings are Aug. 6-10 in St. Louis, MO.
Amanda Lawrence Institute for Imaging and Analytical Technologies Mississippi State University mailto:alawrence-at-i2at.msstate.edu
==============================Original Headers============================== 9, 32 -- From ALawrence-at-i2at.msstate.edu Tue Jun 20 09:48:02 2017 9, 32 -- Received: from chokecherry.its.msstate.edu (chokecherry.its.msstate.edu [130.18.2.120]) 9, 32 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v5KElxLu030786 9, 32 -- for {Microscopy-at-microscopy.com} ; Tue, 20 Jun 2017 09:47:59 -0500 9, 32 -- Received: from mail04.ad.msstate.edu (mail04.ad.msstate.edu [130.18.230.63]) 9, 32 -- by chokecherry.its.msstate.edu (8.13.8/8.13.8) with ESMTP id v5KF23GA013664 9, 32 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=FAIL) 9, 32 -- for {Microscopy-at-microscopy.com} ; Tue, 20 Jun 2017 10:02:04 -0500 9, 32 -- X-Sender: {} 9, 32 -- Received: from MAIL02.ad.msstate.edu (2620:0:1a30:1230::61) by 9, 32 -- mail04.ad.msstate.edu (2620:0:1a30:1230::63) with Microsoft SMTP Server (TLS) 9, 32 -- id 15.0.1236.3; Tue, 20 Jun 2017 10:02:03 -0500 9, 32 -- Received: from MAIL02.ad.msstate.edu ([fe80::7846:3039:9492:24b0]) by 9, 32 -- mail02.ad.msstate.edu ([fe80::7846:3039:9492:24b0%13]) with mapi id 9, 32 -- 15.00.1236.000; Tue, 20 Jun 2017 10:02:03 -0500 9, 32 -- From: "Lawrence, Amanda" {ALawrence-at-i2at.msstate.edu} 9, 32 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 9, 32 -- Subject: Assisting with M&M 2017 meetings 9, 32 -- Thread-Topic: Assisting with M&M 2017 meetings 9, 32 -- Thread-Index: AdLp1A2LsJsgQolbRpajHBt0VOaFPQ== 9, 32 -- Date: Tue, 20 Jun 2017 15:02:03 +0000 9, 32 -- Message-ID: {23d473a805234d4bb9bc0da8633bf5b5-at-mail02.ad.msstate.edu} 9, 32 -- Accept-Language: en-US 9, 32 -- Content-Language: en-US 9, 32 -- X-MS-Has-Attach: 9, 32 -- X-MS-TNEF-Correlator: 9, 32 -- x-ms-exchange-transport-fromentityheader: Hosted 9, 32 -- x-originating-ip: [130.18.230.93] 9, 32 -- Content-Type: text/plain; charset="us-ascii" 9, 32 -- MIME-Version: 1.0 9, 32 -- Content-Transfer-Encoding: 8bit 9, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v5KElxLu030786 ==============================End of - Headers==============================
From kimgarr459-at-gmail.com Tue Jun 20 12:42:48 2017 Return-Path: {kimgarr459-at-gmail.com} Received: from gmail.com ([182.16.74.250]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v5KHgkmM023941 for {microscopylistserverarchive6-at-microscopy.com} ; Tue, 20 Jun 2017 12:42:47 -0500 Message-ID: {B8A6C3F8.3E25F814-at-gmail.com}
I have a client who will be submitting outer surface proteins (OSPs) to resolve by TEM. He plans to use gold conjugated to the primary antibody which will express an antigen on these OSPs. Does anyone have a protocol or know of any older articles related to this? Some concerns and questions I have are: attachment of the OSPs onto a formvar (or parlodian) grid; using a routine IEM procedure or a revised protocol; using a routine negative stain (UA or PTA) or would low angle shadowing work more efficiently? Thank you for any responses to this project! Mary Ard, EM Lab Coordinator University of Georgia, Georgia Electron Microscopy Facility 151 Barrow Hall 115 DW Brooks Drive, UGA Campus Athens, GA 30602 maryard-at-uga.edu
==============================Original Headers============================== 3, 47 -- From maryard-at-uga.edu Wed Jun 21 09:38:54 2017 3, 47 -- Received: from NAM03-BY2-obe.outbound.protection.outlook.com (mail-by2nam03on0127.outbound.protection.outlook.com [104.47.42.127]) 3, 47 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v5LEcrZC020072 3, 47 -- for {Microscopy-at-microscopy.com} ; Wed, 21 Jun 2017 09:38:53 -0500 3, 47 -- Received: from CY1PR02MB2027.namprd02.prod.outlook.com (10.166.190.11) by 3, 47 -- CY1PR02MB2028.namprd02.prod.outlook.com (10.166.190.12) with Microsoft SMTP 3, 47 -- Server (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_128_CBC_SHA256_P256) id 3, 47 -- 15.1.1178.14; Wed, 21 Jun 2017 14:53:00 +0000 3, 47 -- Received: from CY1PR02MB2027.namprd02.prod.outlook.com ([10.166.190.11]) by 3, 47 -- CY1PR02MB2027.namprd02.prod.outlook.com ([10.166.190.11]) with mapi id 3, 47 -- 15.01.1178.023; Wed, 21 Jun 2017 14:53:00 +0000 3, 47 -- From: Mary Brown Ard {maryard-at-uga.edu} 3, 47 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 3, 47 -- Subject: [Microscopy] TEM - gold labeling protein complex 3, 47 -- Thread-Topic: [Microscopy] TEM - gold labeling protein complex 3, 47 -- Thread-Index: AdLqnBrsZZUU/UabR5+jx5Cu1rimrg== 3, 47 -- Date: Wed, 21 Jun 2017 14:53:00 +0000 3, 47 -- Message-ID: {CY1PR02MB2027DAC5729034AC7DC78BB0A3DA0-at-CY1PR02MB2027.namprd02.prod.outlook.com} 3, 47 -- Accept-Language: en-US 3, 47 -- Content-Language: en-US 3, 47 -- X-MS-Has-Attach: 3, 47 -- X-MS-TNEF-Correlator: 3, 47 -- authentication-results: microscopy.com; dkim=none (message not signed) 3, 47 -- header.d=none;microscopy.com; dmarc=none action=none header.from=uga.edu; 3, 47 -- x-originating-ip: [198.137.20.215] 3, 47 -- x-ms-publictraffictype: Email 3, 47 -- x-microsoft-exchange-diagnostics: 1;CY1PR02MB2028;7: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 3, 47 -- x-ms-office365-filtering-correlation-id: f4dc0ea8-1560-4a00-fcec-08d4b8b53660 3, 47 -- x-microsoft-antispam: UriScan:;BCL:0;PCL:0;RULEID:(22001)(2017030254075)(201703131423075)(201703031133081);SRVR:CY1PR02MB2028; 3, 47 -- x-ms-traffictypediagnostic: CY1PR02MB2028: 3, 47 -- x-microsoft-antispam-prvs: {CY1PR02MB2028190899DD6395C7CCC2B6A3DA0-at-CY1PR02MB2028.namprd02.prod.outlook.com} 3, 47 -- x-exchange-antispam-report-test: UriScan:(171495303765807); 3, 47 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:(100000700101)(100105000095)(100000701101)(100105300095)(100000702101)(100105100095)(6040450)(601004)(2401047)(5005006)(8121501046)(93006095)(93001095)(3002001)(10201501046)(100000703101)(100105400095)(6041248)(201703131423075)(201702281529075)(201702281528075)(201703061421075)(201703061406153)(20161123558100)(20161123560025)(20161123562025)(20161123564025)(20161123555025)(6072148)(100000704101)(100105200095)(100000705101)(100105500095);SRVR:CY1PR02MB2028;BCL:0;PCL:0;RULEID:(100000800101)(100110000095)(100000801101)(100110300095)(100000802101)(100110100095)(100000803101)(100110400095)(100000804101)(100110200095)(100000805101)(100110500095);SRVR:CY1PR02MB2028; 3, 47 -- x-forefront-prvs: 0345CFD558 3, 47 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10019020)(6009001)(39450400003)(39840400002)(39410400002)(39850400002)(39400400002)(50986999)(54356999)(6436002)(122556002)(3846002)(81166006)(6116002)(102836003)(6506006)(7736002)(8936002)(2900100001)(8676002)(53936002)(33656002)(75432002)(2906002)(110136004)(38730400002)(88552002)(345774005)(86362001)(2351001)(77096006)(74316002)(5660300001)(6916009)(7696004)(3280700002)(5890100001)(478600001)(2501003)(66066001)(14454004)(25786009)(55016002)(5640700003)(99286003)(189998001)(9686003)(305945005)(3660700001);DIR:OUT;SFP:1102;SCL:1;SRVR:CY1PR02MB2028;H:CY1PR02MB2027.namprd02.prod.outlook.com;FPR:;SPF:None;MLV:sfv;LANG:en; 3, 47 -- spamdiagnosticoutput: 1:99 3, 47 -- spamdiagnosticmetadata: NSPM 3, 47 -- Content-Type: text/plain; charset="us-ascii" 3, 47 -- MIME-Version: 1.0 3, 47 -- X-OriginatorOrg: uga.edu 3, 47 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 21 Jun 2017 14:53:00.6218 3, 47 -- (UTC) 3, 47 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted 3, 47 -- X-MS-Exchange-CrossTenant-id: a8216c1e-4d63-4352-8c3b-50fa1f1475b1 3, 47 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: CY1PR02MB2028 3, 47 -- Content-Transfer-Encoding: 8bit 3, 47 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v5LEcrZC020072 ==============================End of - Headers==============================
For uniform spreading of protein particles on the grid, it is best to have glow-discharged carbon film on top of your formvar or parlodion. If that is not an option, bacitracin solution often helps, or even BSA. Then you can use any basic immunoEM protocol -- which you can obtain from any major supplier or perhaps from community members here. Use minimum blocking first, to establish some binding, then you can include extra blocking steps or higher concentrations, if there is an objectionable background. This usually works quite well for a protein laying on a grid, with no obstacles, like when it is somewhere deep in the cell.
What species is the primary antibody from? If possible, I always preferred to use Protein A -gold as a secondary probe. Much cleaner and more precise. It is best with rabbit polyclonals and will not work with most mouse monoclonals.
Finally, the contrasting step - go with PTA (or ammonium molybdate) over UA: not as dark and spreads easier.
Let me know if you need a sample protocol.
Best wishes, Vlad
Vlad Speransky, PhD Life Sciences Product Specialist Ted Pella, Inc. http://www.tedpella.com/ 530-243-2200 ext. 266 Cell 530-768-3953 vlad_speransky-at-tedpella.com
-----Original Message----- X-from: maryard-at-uga.edu [mailto:maryard-at-uga.edu] Sent: Wednesday, June 21, 2017 7:59 AM To: vlad_speransky-at-tedpella.com
I have a client who will be submitting outer surface proteins (OSPs) to resolve by TEM. He plans to use gold conjugated to the primary antibody which will express an antigen on these OSPs. Does anyone have a protocol or know of any older articles related to this? Some concerns and questions I have are: attachment of the OSPs onto a formvar (or parlodian) grid; using a routine IEM procedure or a revised protocol; using a routine negative stain (UA or PTA) or would low angle shadowing work more efficiently? Thank you for any responses to this project! Mary Ard, EM Lab Coordinator University of Georgia, Georgia Electron Microscopy Facility 151 Barrow Hall 115 DW Brooks Drive, UGA Campus Athens, GA 30602 maryard-at-uga.edu
==============================Original Headers============================== 3, 47 -- From maryard-at-uga.edu Wed Jun 21 09:38:54 2017 3, 47 -- Received: from NAM03-BY2-obe.outbound.protection.outlook.com (mail-by2nam03on0127.outbound.protection.outlook.com [104.47.42.127]) 3, 47 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v5LEcrZC020072 3, 47 -- for {Microscopy-at-microscopy.com} ; Wed, 21 Jun 2017 09:38:53 -0500 3, 47 -- Received: from CY1PR02MB2027.namprd02.prod.outlook.com (10.166.190.11) by 3, 47 -- CY1PR02MB2028.namprd02.prod.outlook.com (10.166.190.12) with Microsoft SMTP 3, 47 -- Server (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_128_CBC_SHA256_P256) id 3, 47 -- 15.1.1178.14; Wed, 21 Jun 2017 14:53:00 +0000 3, 47 -- Received: from CY1PR02MB2027.namprd02.prod.outlook.com ([10.166.190.11]) by 3, 47 -- CY1PR02MB2027.namprd02.prod.outlook.com ([10.166.190.11]) with mapi id 3, 47 -- 15.01.1178.023; Wed, 21 Jun 2017 14:53:00 +0000 3, 47 -- From: Mary Brown Ard {maryard-at-uga.edu} 3, 47 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 3, 47 -- Subject: [Microscopy] TEM - gold labeling protein complex 3, 47 -- Thread-Topic: [Microscopy] TEM - gold labeling protein complex 3, 47 -- Thread-Index: AdLqnBrsZZUU/UabR5+jx5Cu1rimrg== 3, 47 -- Date: Wed, 21 Jun 2017 14:53:00 +0000 3, 47 -- Message-ID: {CY1PR02MB2027DAC5729034AC7DC78BB0A3DA0-at-CY1PR02MB2027.namprd02.prod.outlook. com} 3, 47 -- Accept-Language: en-US 3, 47 -- Content-Language: en-US 3, 47 -- X-MS-Has-Attach: 3, 47 -- X-MS-TNEF-Correlator: 3, 47 -- authentication-results: microscopy.com; dkim=none (message not signed) 3, 47 -- header.d=none;microscopy.com; dmarc=none action=none header.from=uga.edu; 3, 47 -- x-originating-ip: [198.137.20.215] 3, 47 -- x-ms-publictraffictype: Email 3, 47 -- x-microsoft-exchange-diagnostics: 1;CY1PR02MB2028;7:kjMIar6CPk15617CIlFU4UujV6pMed6AC+3jePxXC9ZM9M3epdNGbMNKUW BDWDg3x20EMWRS6xE9JH6gkT5/5f+r9L6URXhpyKyaQuVu/Y10u4AVB/0apJM74W2AY4DQXNMrbl hB5cAQGfdvOis9sgxPCw+tqUbJ972+FnbvrK7dn2ZFu2Bc1BavxxxPVxLvL4ZA4oCk0yjM16rnGe 5ILpsBo4JRYi5xY7/AS/7BBikZrVZxc/ChMC5LPCqdFvo6yQdTG42Hl2r4InPur+btJSUmmBRrsL 6RnNz4UH6ohahylRi+rFjAiBi2sBvWeP5y8smebbs69qCZLujR6xkzrqm+6n5w2qvVKRq0UmSaAO mablhRPYY3BnjS3A2S8Aykls6FQolAucUAG836tvzW7S8tyzLudKOZV7VShDZfOuDaPAi08p40K+ hjIl16n2mfXML6kJFiaFZ6t6AX+fiHYpJSVRGs/sr+70UD9Dg4ICIzRL1axMX1TFQ/VAsNI6zxBw I5zYh2YiR3e0MRgPuohxXIfDkXFh3z0GB0MCZmgdX0LcSkfcJrmHTRF4vSFkKXcqRncSSht/SuQV azdJPCpzFPuK5w2rM8bpe1m7wlFyuVz14LoTVu7pL45hNO6700P+EEsFEXoKEiqa/8cruS6K26+N 3m/MeRojtDzBx70TH4QQNOVGFoJ2kzfnghJxLv8zWcvHPYLsyLMrSs1wp6WfeVTzG0ak+JuESOG6 4CT/tPEhGpt0sx8EMOhSdABLyhOp3wMzqBLhghDFLZnBNvfwfHK14cOFMDGEa4vjrEFyI= 3, 47 -- x-ms-office365-filtering-correlation-id: f4dc0ea8-1560-4a00-fcec-08d4b8b53660 3, 47 -- x-microsoft-antispam: UriScan:;BCL:0;PCL:0;RULEID:(22001)(2017030254075)(201703131423075)(20170303 1133081);SRVR:CY1PR02MB2028; 3, 47 -- x-ms-traffictypediagnostic: CY1PR02MB2028: 3, 47 -- x-microsoft-antispam-prvs: {CY1PR02MB2028190899DD6395C7CCC2B6A3DA0-at-CY1PR02MB2028.namprd02.prod.outlook. com} 3, 47 -- x-exchange-antispam-report-test: UriScan:(171495303765807); 3, 47 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:(100000700101)(100105000095)(100000701101)(100105300095)( 100000702101)(100105100095)(6040450)(601004)(2401047)(5005006)(8121501046)(9 3006095)(93001095)(3002001)(10201501046)(100000703101)(100105400095)(6041248 )(201703131423075)(201702281529075)(201702281528075)(201703061421075)(201703 061406153)(20161123558100)(20161123560025)(20161123562025)(20161123564025)(2 0161123555025)(6072148)(100000704101)(100105200095)(100000705101)(1001055000 95);SRVR:CY1PR02MB2028;BCL:0;PCL:0;RULEID:(100000800101)(100110000095)(10000 0801101)(100110300095)(100000802101)(100110100095)(100000803101)(10011040009 5)(100000804101)(100110200095)(100000805101)(100110500095);SRVR:CY1PR02MB202 8; 3, 47 -- x-forefront-prvs: 0345CFD558 3, 47 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10019020)(6009001)(39450400003)(39840400002)(39410400002)(3985 0400002)(39400400002)(50986999)(54356999)(6436002)(122556002)(3846002)(81166 006)(6116002)(102836003)(6506006)(7736002)(8936002)(2900100001)(8676002)(539 36002)(33656002)(75432002)(2906002)(110136004)(38730400002)(88552002)(345774 005)(86362001)(2351001)(77096006)(74316002)(5660300001)(6916009)(7696004)(32 80700002)(5890100001)(478600001)(2501003)(66066001)(14454004)(25786009)(5501 6002)(5640700003)(99286003)(189998001)(9686003)(305945005)(3660700001);DIR:O UT;SFP:1102;SCL:1;SRVR:CY1PR02MB2028;H:CY1PR02MB2027.namprd02.prod.outlook.c om;FPR:;SPF:None;MLV:sfv;LANG:en; 3, 47 -- spamdiagnosticoutput: 1:99 3, 47 -- spamdiagnosticmetadata: NSPM 3, 47 -- Content-Type: text/plain; charset="us-ascii" 3, 47 -- MIME-Version: 1.0 3, 47 -- X-OriginatorOrg: uga.edu 3, 47 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 21 Jun 2017 14:53:00.6218 3, 47 -- (UTC) 3, 47 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted 3, 47 -- X-MS-Exchange-CrossTenant-id: a8216c1e-4d63-4352-8c3b-50fa1f1475b1 3, 47 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: CY1PR02MB2028 3, 47 -- Content-Transfer-Encoding: 8bit 3, 47 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v5LEcrZC020072 ==============================End of - Headers==============================
==============================Original Headers============================== 21, 22 -- From vlad_speransky-at-tedpella.com Wed Jun 21 14:14:58 2017 21, 22 -- Received: from asmx01.boltonsmith.com (asmx01.boltonsmith.com [69.90.54.3]) 21, 22 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v5LJEwNY015245 21, 22 -- for {Microscopy-at-microscopy.com} ; Wed, 21 Jun 2017 14:14:58 -0500 21, 22 -- X-Virus-Scanned: by SpamTitan at boltonsmith.net 21, 22 -- Received: from PELLAMS266 [12.7.209.242] by intown.net with ESMTP 21, 22 -- (SMTPD-12.0.0.384) id fbfa00079c5d3669; Wed, 21 Jun 2017 15:29:01 -0400 21, 22 -- From: "Vlad Speransky" {vlad_speransky-at-tedpella.com} 21, 22 -- To: {Microscopy-at-microscopy.com} 21, 22 -- Cc: {maryard-at-uga.edu} 21, 22 -- References: {201706211459.v5LEx9jf007464-at-microscopy.com} 21, 22 -- In-Reply-To: {201706211459.v5LEx9jf007464-at-microscopy.com} 21, 22 -- Subject: RE: [Microscopy] TEM - gold labeling protein complex 21, 22 -- Date: Wed, 21 Jun 2017 12:29:05 -0700 21, 22 -- Message-ID: {007901d2eac4$a5e6c660$f1b45320$-at-tedpella.com} 21, 22 -- MIME-Version: 1.0 21, 22 -- Content-Type: text/plain; 21, 22 -- charset="us-ascii" 21, 22 -- Content-Transfer-Encoding: 7bit 21, 22 -- X-Mailer: Microsoft Outlook 14.0 21, 22 -- Thread-Index: AQJ9Rns3P176UniaygJej6r47Xg6e6Da+M2Q 21, 22 -- Content-Language: en-us ==============================End of - Headers==============================
In the process of cleaning the lab, I came across a what appears to be a complete MT-2 instruction manual. Any takers? Let me know within a week or into the dumpster she goes.
Jim
--
James M. Ehrman Digital Microscopy Facility Mount Allison University 63B York St. Sackville, NB E4L 1G7 CANADA
Two silk worms had a race. They ended up in a tie.
==============================Original Headers============================== 8, 21 -- From jehrman-at-mta.ca Thu Jun 22 06:52:56 2017 8, 21 -- Received: from smtpy.mta.ca (smtpx.mta.ca [198.164.44.42]) 8, 21 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v5MBqueg029124 8, 21 -- for {Microscopy-at-microscopy.com} ; Thu, 22 Jun 2017 06:52:56 -0500 8, 21 -- Received: from [138.73.22.234] (port=56195) 8, 21 -- by smtpy.mta.ca with esmtpsa (TLSv1.2:ECDHE-RSA-AES128-GCM-SHA256:128) 8, 21 -- (Exim 4.87_1) 8, 21 -- (envelope-from {jehrman-at-mta.ca} ) 8, 21 -- id 1dO0to-0003ll-Lt 8, 21 -- for Microscopy-at-microscopy.com; Thu, 22 Jun 2017 09:07:48 -0300 8, 21 -- To: Microscopy Listserv {Microscopy-at-microscopy.com} 8, 21 -- From: "James M. Ehrman" {jehrman-at-mta.ca} 8, 21 -- Subject: Free! Operating Instructions for Sorvall MT-2 8, 21 -- Message-ID: {a8f89d10-b053-54f9-35fd-bade826d5480-at-mta.ca} 8, 21 -- Date: Thu, 22 Jun 2017 09:06:57 -0300 8, 21 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:52.0) Gecko/20100101 8, 21 -- Thunderbird/52.2.0 8, 21 -- MIME-Version: 1.0 8, 21 -- Content-Type: text/plain; charset=utf-8; format=flowed 8, 21 -- Content-Transfer-Encoding: 7bit 8, 21 -- Content-Language: en-US ==============================End of - Headers==============================
From thivisid08-at-gmail.com Fri Jun 23 19:34:15 2017 Return-Path: {thivisid08-at-gmail.com} Received: from gmail.com ([103.194.187.218]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v5O0YDDZ013370 for {microscopylistserverarchive6-at-microscopy.com} ; Fri, 23 Jun 2017 19:34:14 -0500 Message-ID: {1AB27A09.23044D63-at-gmail.com}
Dear All,
From the lectures and discussions at the recent 3DEM GRC (Les Diablerets, 2017), I noticed there are still serious misunderstandings – even among distinguished professors in Physics and in Biology - on what “resolution” actually means. So allow me to go over the basic principles:
1) The instrumental resolution of an imaging system is given by the physical properties of the microscope, telescope, photographic camera or whatever your favorite 1D-, 2D-, 3D-, 4D-imaging device is. The classical case would be that of a light microscope where the numerical aperture (NA) of the objective lens (https://en.wikipedia.org/wiki/Numerical_aperture) determines the “instrumental resolution” of the microscope (https://en.wikipedia.org/wiki/Angular_resolution). In the case of a diffraction-limited telescope it is the diameter of the main lens that determines the instrumental resolution. In the old days of Electron Microscopy one would often see the first zero of the CTF being used to define the instrumental resolution of the microscope.
2) The resolution achieved for the results based on the images collected is a very different issue! Suppose, for example, you forget to switch on the illumination of your light microscope! What good will then the high-resolution (high NA) properties of your expensive instrument do you? If, on the other hand, you do switch on the illumination but only use a very low dose of ~ 10,000 photons to generate an image, that image will be very noisy. How much better will the image of your object be if the image is created accumulating a total of 10,000,000,000 photons? The underlying question is: how do I define a results-oriented quality metric that reflects the image information I have collected in an experiment rather than what a specific instrument can potentially collect?
The basic idea is to take TWO images of the same object rather than just ONE. Both images will contain the same signal (the object) but a different realization of the random noise so we can then compare the two images to each other in Fourier space using the FRC (Fourier Ring Correlation). This suggestion first emerged in single-particle EM in the early 1980s (https://en.wikipedia.org/wiki/Fourier_shell_correlation).
Strangely enough it took decades for the rest of the imaging scientists to realize what they were missing. Only very recently “everybody” suddenly started using the results-oriented FRC and FSC metrics in many other imaging fields, including X-ray microscopy, X-ray crystallography, light-microscopy, X-ray tomography, scanning microscopy, astronomical imaging, etc.
Instead of claiming “super resolution” by showing some nice images from a given microscope, one can now just prove it through an FRC/FSC curve. I never understood why it took everybody so long to adapt to this straightforward gold-standard metric.
Take home lesson: the “INSTRUMENTAL RESOLUTION” is the intrinsic resolution that the instrument is capable of, whether you actually use it or whether you leave it in the cupboard. The statistically significant “RESULTS RESOLUTION”, on the other hand, reflects the quality of the final results achieved within a given data-collection experiment. These are TWO very different concepts!
My TWO cents,
Marin
=====================================
Prof Dr Ir Marin van Heel Research Professor at: Laboratório Nacional de Nanotecnologia - LNNano CNPEM/LNNano, Campinas, Brazil Emeritus Professor, Imperial College London Emeritus Professor, Leiden University Skype: marin.van.heel
From aaronlittle895-at-gmail.com Sun Jun 25 08:12:46 2017 Return-Path: {aaronlittle895-at-gmail.com} Received: from gmail.com ([221.160.34.117]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v5PDCifY030345 for {microscopylistserverarchive6-at-microscopy.com} ; Sun, 25 Jun 2017 08:12:45 -0500 Message-ID: {D1F5A874.ECCF41FB-at-gmail.com}
We are thinking about buying a cryostat for our core facility. Some clients really want the cryojane add on kit. Do any of you use this in a core setting? What do you charge for consumables?
How important do you think a motorized drive is? One or two clients want this expensive upgrade - does it get used by the average person? Is it worth the money?
Leica has a vacuum assist in their 1950 model that aids in sectioning. I haven't seen the demo yet but intend to. Any fans of this technology?
Thanks. Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Curator's Distinguished Teaching Professor Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
Hi, we have ordered one with Cryojane tape transfer system on Leica CM3050S 2 -3 years ago but in vain as the company was not able to do the successful DEMO even after multiple efforts finally it had to be used without the cryojane system also the accessories will cost you lot, I think only LEICA only claims to have this technology
thanks,
Pundir (TO) National Brain Research Centre Manesar, Gurgaon HARYANA INDIA
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
We are thinking about buying a cryostat for our core facility. Some clients really want the cryojane add on kit. Do any of you use this in a core setting? What do you charge for consumables?
How important do you think a motorized drive is? One or two clients want this expensive upgrade - does it get used by the average person? Is it worth the money?
Leica has a vacuum assist in their 1950 model that aids in sectioning. I haven't seen the demo yet but intend to. Any fans of this technology?
Thanks. Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Curator's Distinguished Teaching Professor Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
RMC-Boeckeler makes a tape-transfer system for their microtomes with cryo.
Phil ------------- Philip Oshel Imaging Facility Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 989 774-3576 office 989 774-7567 lab
Hi, we have ordered one with Cryojane tape transfer system on Leica CM3050S 2 -3 years ago but in vain as the company was not able to do the successful DEMO even after multiple efforts finally it had to be used without the cryojane system also the accessories will cost you lot, I think only LEICA only claims to have this technology
thanks,
Pundir (TO) National Brain Research Centre Manesar, Gurgaon HARYANA INDIA
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
We are thinking about buying a cryostat for our core facility. Some clients really want the cryojane add on kit. Do any of you use this in a core setting? What do you charge for consumables?
How important do you think a motorized drive is? One or two clients want this expensive upgrade - does it get used by the average person? Is it worth the money?
Leica has a vacuum assist in their 1950 model that aids in sectioning. I haven't seen the demo yet but intend to. Any fans of this technology?
Thanks. Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Curator's Distinguished Teaching Professor Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
From otiscatr45-at-gmail.com Sat Jul 1 23:44:58 2017 Return-Path: {otiscatr45-at-gmail.com} Received: from gmail.com (59-100-3-189.syd.static-ipl.aapt.com.au [59.100.3.189]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v624ismW024978 for {microscopylistserverarchive6-at-microscopy.com} ; Sat, 1 Jul 2017 23:44:57 -0500 Message-ID: {E0E0153E.DF077792-at-gmail.com}
Has anyone found a source for this dehydration agent? Can you share the source ID with the List? I need this agent to prep some bio specimens the right way.
Your help and insight is much appreciated.
gary g.
--- This email has been checked for viruses by Avast antivirus software. https://www.avast.com/antivirus
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both lbochtler-at-protonmail.ch as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: lbochtler-at-protonmail.ch Name: Lukas B.
Title-Subject: [Filtered] Hitachi S800 Manual and Service manual Request
Message: Hello,
I just scored a Hitachi S800 electron microscope, sadly most cables between the main console (with the electron column) and the control desk have been cut. I can replace most if not all cables, so that isn't much of a problem.
However, i did not get any documentation with it, since i don't know if the original owner will ever manage to find any documentation for it, i decided to ask you guys.
Ideally i would need both the user and service manual for the microscope, possibly with the computer software (since im not sure if i can get the computer for it as well, since the microscope and the other parts are now in 2 different locations)
Ideally id also need the power requirements for the microscope, what voltage dose it need, how much power dose it draw, and how many power phases dose it need.
Best regards,
Lukas B.
Login Host: 62.245.208.5 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
just to inform (knowing there might have been meanwhile a lot of personal replies to you): hope to have understood your request correctly. almost all bigger chemical sellers*) have DMP-Dimethoxypropane in stock (I guess youll use it for rapid dehydration of biological specimens avoiding the common dehydration by ascending ethanol or acetone steps ( acidified DMP will dehydrate small biological specimens by cleaving tissular water molecules into methanol and acetone).
----------------------------------------------- Science Lab: www.sciencelab.com/msds.php?msdsId=9923794 Sciencelab.com, Inc. 14025 Smith Rd. Houston, Texas 77396 US Sales: 1-800-901-7247 International Sales: 1-281-441-4400 Order Online: ScienceLab.com
----------------------------------------------- SIGMA-ALDRICH (for Austria: http://www.sigmaaldrich.com/catalog/product/sial/d136808?lang=de®ion=AT&g clid=CMKbk-yD7dQCFdAV0wodKHwCnA Globally: www.sigmaaldrich.com Austria Home D136808 Sigma-Aldrich 2,2-Dimethoxypropane reagent grade, 98% Synonym: Acetone dimethyl acetal - CAS Number 77-76-9 - Linear Formula (CH3)2C(OCH3)2 - Molecular Weight 104.15 - Beilstein Registry Number 635678 - EC Number 201-056-0 - MDL number MFCD00008479 - PubChem Substance ID 24893272
Related Categories Analytical/Chromatography, Applications, Chemical Synthesis, Derivatization of Fatty Acids to FAMEs, Fats (fatty acids, FAMEs, glycerides), Edible Oils, Sterols, More... Grade: reagent grade vapor density: 3.59 (vs air) vapor pressure: 60 mmHg ( 15.8 C) InChI Key: HEWZVZIVELJPQZ-UHFFFAOYSA-N Assay: 98% expl. lim. 31%, 58F 6%, 27F
Packaging 18 L in steel drum 25, 500 mL in glass bottle 2.5 L in glass bottle Application Reagent for the preparation of 1,2-diols as acetonides.[3] General description 2,2-Dimethoxypropane (DMP) is an organic building block commonly employed as a precursor to generate 2-methoxypropene (MPP). The degradation study of DMP in ionic liquids showed the formation of MPP and 2-ethoxypropene (EPP) in an identical ratio due to the tunneling effect.[4] Conformational analysis of DMP based on ab initio calculations and matrix isolation infrared spectroscopy has been reported.[1] DMP reacts with water to produce methanol and acetone. This reaction has been employed in a method for the quantification of water in natural products by gas-liquid chromatography.[5] Acidified DMP has been employed for the dehydration of biological samples.[2]
----------------------------------------------- http://www.merckmillipore.com/AT/de/product/2%2C2-Dimethoxypropane,MDA_CHEM- 802936?ReferrerURL=https%3A%2F%2Fwww.google.com%2F 802936 | 2,2-Dimethoxypropan - Download - Zoom 2,2-Dimethoxypropane for synthesis. CAS 77-76-9, molar mass 104.15g/mol. 2,2-Dimethoxypropan MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
----------------------------------------------- ALFA AESAR: https://www.alfa.com/en/catalog/A13810/ A13810 2,2-Dimethoxypropane, 98% Use: 2,2-Dimethoxypropane acts as a dehydrating agent. It also serves as an intermediate in the synthesis of vitamin E, vitamin A and various carotenoids such as astaxanthin. It is used as a reagent for the preparation of 1,2-diols, acetonides, isopropylidene derivatives of sugars, nucleosides, methyl esters of amino acids and enol ethers. Notice: Incompatible with oxidizing agents and acids.
============================================================================ ========== Disclaimer: Just to be honest: no affiliation with / no financial interest in any of the mentioned companies. I have used acidified DMP in/for the rapid dehydration of small biological (human) tissue specimens in diagnostic TEM for nearly 30 years with really good success (some points may be critical and perhaps need to be mentioned at least) and do have some (routine/standard recipes for that)
Literature (only a selection out of many): CONWAY K 1999, https://www.ncbi.nlm.nih.gov/pubmed/10190257 DeRUITER A, VanBANNING P& WILLEMSE JJ (1981): Brief technical note Rapid histological results in aquaculture research by using the time-saving embedding procedure with 2,2-dimethoxypropane ( Aquaculture, Volume 25, Issues 23, August 1981, Pages 293-297) see: http://www.sciencedirect.com/science/article/pii/0044848681901939 KAESER W. 1989. Freeze substitution of plant tissues with a new medium containing dimethoxypropane. J. Microsc. 154: 273-278 LIN C.H., Falk R.H. and Stocking C.R. 1977. Rapid chemical Ddehydration of plant material for light and electron microscopy with 2,2-dimethoxypropane and 2,2-diethoxypropane. Am. J. Bot.64: 602-605 MASER D.M. and TRIMBLE III, J.J. 1977. Rapid chemical dehydration of biologic samples for scanning electron microscopy using 2,2-dimethoxypropane. J. Histochem. Cytochem. 25:247-251 MASON M, MACKIE RM (1985): Comparative study of three methods of plastic embedding in diagnostic dermatopathology JClinPathol1985;38:1397-1399,
(https://www.ncbi.nlm.nih.gov/pmc/articles/PMC499499/pdf/jclinpath00195-0077 .pdf) MLLER W. and MLLER G. 1994. Chemical dehydration for rapid paraffin embedding. Biotech. & Histochem. 69: 289-290 MULLER L.L. and JACKS T.J. 1975. Rapid chemical dehydration of samples for electronmicroscopic examinations. J. Histochem. Cytochem. 23: 107-110 A Pernstich, H W Krenn, G Pass(2003): Preparation of serial sections of arthropods using 2,2-dimethoxypropane dehydration and epoxy resin embedding under vacuum. Biotechnic & Histochemistry, Jan 2003, Vol. 78, No. 1, Pages 5-9. ( http://www.tandfonline.com/doi/abs/10.1080/10520290312120002 )
POSTEK M. T. and TUCKER S. C. 1976. A new short chemical dehydration method for light microscopy preparations of plant material. Can. J. Bot. 54, 872-875
Best wishes and regards,
MUSS Wolfgang Dr.phil. (PhD) [OR i. R. / en retraite / retired] Ignaz-Rieder-Kai 19/6 A-5020 SALZBURG sterreich-AUSTRIA Mobil-Tel.: 0043(0)676 5 369 456 FN-Tel. m. Anrufbeantw.: 004(0)662 62 91 46 E-mail: wij.muss-at-aon.at E-Mail altern.: womuss-at-gmail.com
FRMS, Retired Member of MSA
Scientific Profile at ResearchGate: http://www.researchgate.net/profile/Wolfgang_MUSS Former Head of Electron Microscopy Lab at Institute of Pathology SALK-LKH / Salzburger Landeskliniken | General Hospital and PMU (private) PARACELSUS MEDICAL UNIVERSITY of SALZBURG
Former Secretary and (until June2017) Board Member of the
SCUR {The Society for Cutaneous Ultrastructure Research} The Skin Imaging Society { www.scur.org }
==============================Original Headers============================== 33, 21 -- From wij.muss-at-aon.at Mon Jul 3 08:55:25 2017 33, 21 -- Received: from bsmtp7.bon.at (bsmtp7.bon.at [213.33.87.19]) 33, 21 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v63DtOOa019171 33, 21 -- for {microscopy-at-microscopy.com} ; Mon, 3 Jul 2017 08:55:25 -0500 33, 21 -- Received: from MussTHINK (unknown [93.83.25.98]) 33, 21 -- by bsmtp7.bon.at (Postfix) with ESMTPSA id 3x1TYP0SLzz5tlS; 33, 21 -- Mon, 3 Jul 2017 16:10:16 +0200 (CEST) 33, 21 -- From: "MUSS Wolfgang Dr. phil./PhD \(OR i.R, retired\)" {wij.muss-at-aon.at} 33, 21 -- To: {microscopy-at-microscopy.com} 33, 21 -- Cc: {gary-at-gaugler.com} 33, 21 -- Subject: [Microscopy] Re: Dimethoxypropane source 33, 21 -- Date: Mon, 3 Jul 2017 16:10:17 +0200 33, 21 -- Message-ID: {000d01d2f406$1986a000$4c93e000$-at-aon.at} 33, 21 -- MIME-Version: 1.0 33, 21 -- Content-Type: text/plain; 33, 21 -- charset="iso-8859-1" 33, 21 -- X-Mailer: Microsoft Outlook 15.0 33, 21 -- Thread-Index: AdL0BhibKy+BU+DJTXCjNSWoYLOmtw== 33, 21 -- Content-Language: de 33, 21 -- Content-Transfer-Encoding: 8bit 33, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v63DtOOa019171 ==============================End of - Headers==============================
From wandjoan59-at-gmail.com Mon Jul 3 23:21:54 2017 Return-Path: {wandjoan59-at-gmail.com} Received: from gmail.com ([218.232.94.109]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v644LpVd004691 for {microscopylistserverarchive6-at-microscopy.com} ; Mon, 3 Jul 2017 23:21:53 -0500 Message-ID: {2D5269CB.D662C823-at-gmail.com}
X-from: thomas.glaeser-at-leica-microsystems.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both thomas.glaeser-at-leica-microsystems.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: thomas.glaeser-at-leica-microsystems.com Name: Thomas Glaeser
Message: For our Product Management team in Vienna we are looking for a strong personality as
Product Manager Nanotechnologie (f/m)
Key Responsibilities:
Manage the product life cycle for assigned products within the Biological portfolio Project management of internal efforts required for product commercialization, including interfacing with Quality Management Systems, R&D, and related roles Organize and drive activities cross functionally related to global product launch, including developing positioning, sales training, pricing and collateral development to ensure product launch execution Interact with key internal and external customers globally to understand problems, obtain feedback on new and existing products, and identify solutions Specify market requirements from all major geographic regions supported by on-going market research with customers and non-customers Monitors financial performance of the (revenue, profitability) market, and establishes KPIs for the product lines financial performance and develops countermeasures when performance is not met Key Requirements:
Bachelor's degree in Science, Engineering, Marketing, Business Administration, or equivalent experience is required. PhD or MBA is preferred Minimum 3-5 years of experience in research / sample preparation of biological samples Strong knowledge of EM and EM sample preparation with cryo methods Experience in product management, marketing preferred Proficient in English and German (spoken & written), other languages (is a plus)
Are you interested to work independently in a team orientated environment, do you want to go new ways and solve innovative issues? Please apply directly at https://danaher.taleo.net/careersection/jobdetail.ftl?job=NAN000025&lang=en or send me an email.
Login Host: 195.63.85.50 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
-- =========================================== Do not reply to this message it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to
I have an assortment of new apertures for a JEOL 100CX II. They will also fit a 100CX. The assortment includes fixed condenser apertures and condenser and objective aperture strips. They are yours for the asking. Just pay shipping.
Frank Macaluso Senior Associate in Anatomy and Structural Biology Administrative Director and Director of Electron Microscopy Analytical Imaging Facility Albert Einstein College of Medicine 1300 Morris Park Avenue Bronx, NY 10461
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both rcsencsits-at-belcan.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Organization: Schafer Corporation-A Belcan Company
Title-Subject: [Filtered] Aspex Controller box and Stage
Message: Does anyone have an Aspex 3025 joystick controller box and/or stage? I am looking for the Aspex Explorer 80 x 100 stage and controller box. the stage is motorized x,y and manual z. I believe many have been decommissioned and I am interested in parts. Thanks, Roseann
Login Host: 12.49.221.194 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mtg2003-at-med.cornell.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: mtg2003-at-med.cornell.edu Name: Michael Ganger
Organization: Weill Cornell Medicine
Title-Subject: [Filtered] EMBED 812 Bubbles
Message: Hi quick question for everyone. I usually cure my EMBED 812 resin in a vacuum oven at 15mmHg at 60C. The oven had failed facilitating a new purchase. After balancing the temperature, I put some test blocks in without tissue to just test the polymerization and cutting post cure.
To my dismay, I have been having bubbles form in the capsules (BEEM 00), and they occur along the edges from the bottom to the top of capsule. It does not matter where I put the trays, or the number of capsules to polymerize. I have varied the vacuum from 10mmHg (bubbles are finer and worse) to 20 mmHg (bubbles form at the bevel to the tip and are slightly larger in size.)I have yet to vary the temp.
I've been using vacuum ovens for over 16 years and have never had any problems before and I'm a bit stumped. Any help would be most appreciated.
Thanks in advance,
Mike Weill Cornell Medicine NY, NY
Login Host: 140.251.32.88 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
From skarbnik-at-ugdl.pl Sun Jul 9 03:10:56 2017 Return-Path: {skarbnik-at-ugdl.pl} Received: from mail.joeclinic.jp (mail.joeclinic.jp [203.137.91.215]) by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v698AsJJ010878; Sun, 9 Jul 2017 03:10:55 -0500 Received: from server.corpsupport.com (tsc-ded-csi-cs-1.ded.bright.net [216.255.16.146]) (authenticated (0 bits)) by mail.joeclinic.jp (8.14.3/8.11.3) with ESMTP id v698I1Qi019909; Sun, 9 Jul 2017 17:22:43 +0900 Message-Id: {201707090822.v698I1Qi019909-at-mail.joeclinic.jp} Content-Type: multipart/alternative; boundary="===============1509147478==" MIME-Version: 1.0
X-from: D.Strachan-at-Beatson.gla.ac.uk
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both D.Strachan-at-Beatson.gla.ac.uk as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: D.Strachan-at-Beatson.gla.ac.uk Name: David Strachan
Organization: Beatson Institute for Cancer Research
Title-Subject: [Filtered] Alternatives to Essen BioScience Incucyte
Message: Dear List,
I was wondering if anyone has any thoughts on alternatives to the Essen Bioscience Incucyte system. Essentially we would like a system that can accommodate multi-well plates, image for up to one week, so probably placed inside an incubator or have its own specialised environmental control. Be capable of phase contrast or other I.C. technique and have a minimum of two colour fluorescence.
The new incucyte S3 now has an over-inflated price hike, at least in the U.K., so is no longer looking like something we will be considering.
Any thoughts greatly appreciated.
regards
David Strachan Senior Scientific Officer Beatson Institute for Cancer Research UK
Login Host: 130.209.6.42 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
Dear All During SEM examination of human endometrium I found a filamentous structure resembling bacteria. The way it contacts the microvilli of the epithelial cells looks like this object was there before fixation, and I can see constrictions (sulci) along the filament. So I think it is a colony but I would like to ask your opinion, as some of you are very strong in observing bacteria with SEM. I could not take higher resolution images because the sample has difficulties and the electron beam was drifting. Please have a look:
Tel/fax +30 210 8957677 mobile +30 6945 107477 www.eikonika.netwww.aim.cat *************************************
==============================Original Headers============================== 8, 20 -- From eikonika-at-otenet.gr Mon Jul 10 14:38:39 2017 8, 20 -- Received: from chimaera.otenet.gr (smtp-out32.otenet.gr [83.235.69.32]) 8, 20 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v6AJcd6B026903 8, 20 -- for {microscopy-at-microscopy.com} ; Mon, 10 Jul 2017 14:38:39 -0500 8, 20 -- Received: from ozymandias (ppp-94-64-220-7.home.otenet.gr [94.64.220.7]) 8, 20 -- by chimaera.otenet.gr (ESMTP) with ESMTPSA 8, 20 -- for {microscopy-at-microscopy.com} ; Mon, 10 Jul 2017 22:53:56 +0300 (EEST) 8, 20 -- From: "Yorgos Nikas" {eikonika-at-otenet.gr} 8, 20 -- To: {microscopy-at-microscopy.com} 8, 20 -- Subject: filamentous bacteria? 8, 20 -- Date: Mon, 10 Jul 2017 22:53:54 +0300 8, 20 -- Message-ID: {000001d2f9b6$4299b760$c7cd2620$-at-otenet.gr} 8, 20 -- MIME-Version: 1.0 8, 20 -- Content-Type: text/plain; 8, 20 -- charset="iso-8859-1" 8, 20 -- X-Mailer: Microsoft Outlook 14.0 8, 20 -- Thread-Index: AdL5s78sWmEwqlwwTByswDSUlwNVzg== 8, 20 -- Content-Language: en-us 8, 20 -- Content-Transfer-Encoding: 8bit 8, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v6AJcd6B026903 ==============================End of - Headers==============================
From ebeldong6-at-gmail.com Mon Jul 10 15:01:25 2017 Return-Path: {ebeldong6-at-gmail.com} Received: from gmail.com ([180.178.40.50]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v6AK1MMr017691 for {microscopylistserverarchive6-at-microscopy.com} ; Mon, 10 Jul 2017 15:01:23 -0500 Message-ID: {6875F14E.882AB665-at-gmail.com}
Dear Colleagues,
If anyone has expertise on the EM-300 TEM, please contact me offline. Specifically, I believe I have a water flow issue (the vacuum ON light remains illuminated) and a vacuum problem related to the camera airlock (possibly one of the microswitches that interface with the airlock mechanism/rotary knob).
Thanks,
Steve
Stephen J. Beck Professor Coordinator, Bio-Imaging Center Electron Microscopy Department of Biology Nassau Community College Garden City, NY 11530
SMECC Museum project in Arizona is looking for Manuals, Parts, photos of installations and people using them and peoples memories of the RCA EMT (EMT-3)
Greetings, in our many year effort to document the RCA EMT Entry level Electron Microscope we are looking basically for anything related to it including memories and folklore.
Please drop us a line off list. if you have any of the above to share.
Many thanks in advance.... Ed Sharpe Archivist for SMECC www.smecc.org
==============================Original Headers============================== 4, 29 -- From COURYHOUSE-at-aol.com Mon Jul 10 15:57:58 2017 4, 29 -- Received: from omr-a010e.mx.aol.com (omr-a010e.mx.aol.com [204.29.186.54]) 4, 29 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v6AKvw8T010770 4, 29 -- for {microscopy-at-microscopy.com} ; Mon, 10 Jul 2017 15:57:58 -0500 4, 29 -- Received: from mtaomg-aag01.mx.aol.com (mtaomg-aag01.mx.aol.com [172.26.126.79]) 4, 29 -- by omr-a010e.mx.aol.com (Outbound Mail Relay) with ESMTP id D0FFF38000BE 4, 29 -- for {microscopy-at-microscopy.com} ; Mon, 10 Jul 2017 17:13:18 -0400 (EDT) 4, 29 -- Received: from core-aba01b.mail.aol.com (core-aba01.mail.aol.com [172.27.22.1]) 4, 29 -- by mtaomg-aag01.mx.aol.com (OMAG/Core Interface) with ESMTP id 7E00B38000084 4, 29 -- for {microscopy-at-microscopy.com} ; Mon, 10 Jul 2017 17:13:18 -0400 (EDT) 4, 29 -- From: COURYHOUSE-at-aol.com 4, 29 -- Message-ID: {1918e6.5f1f5018.469547ee-at-aol.com} 4, 29 -- Date: Mon, 10 Jul 2017 17:13:18 -0400 4, 29 -- Subject: Looking for Manuals, Parts and peoples memories of the RCA EMT (EMT-3) 4, 29 -- To: microscopy-at-microscopy.com 4, 29 -- MIME-Version: 1.0 4, 29 -- Content-Type: text/plain; charset="US-ASCII" 4, 29 -- Content-Transfer-Encoding: 7bit 4, 29 -- X-Mailer: AOL 9.8 sub 2024 4, 29 -- X-Originating-IP: [72.222.165.60] 4, 29 -- x-aol-global-disposition: G 4, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=mx.aol.com; 4, 29 -- s=20150623; t=1499721198; 4, 29 -- bh=M8JM8I8JNCvtLq5rVfF3yXBidSAdgZEnt1pRSFw7Blo=; 4, 29 -- h=From:To:Subject:Message-ID:Date:MIME-Version:Content-Type; 4, 29 -- b=M01rAvw9e2CwJYxQARO+FGO69cnyQtoXLldNH5/0nC+HY0PcEBGRAMrBaXHQ3MFhF 4, 29 -- G71HrE/1Wr+MIaNK/gNTzG6iDFWmptG7tAH68n6zD8gBQ4dVhoSeC3hjlWNhXe7wHx 4, 29 -- dc8fwih01dkho2qcoxbp82LvTlBNozFIkQkYKwk4= 4, 29 -- x-aol-sid: 3039ac1a7e4f5963edee44fb ==============================End of - Headers==============================
We would like to draw your attention to a new Master of Science course starting in National University of Ireland Galway. This is an exciting new course and will benefit from the cutting edge facilities available from the Centre for Microscopy and Imaging at NUIG. At a time when we can view and control the very actions of cells using light, follow the development of cells in vivo and even visualise individual molecular interactions in a biological context this course will equip students with invaluable skills for a multitude of careers and research applications.
NUIG is ranked among the top 1% of universities globally in the QS World University Ranking 2017/18, and this ranking has been consistently rising for the past 5 years. Galway as a city is a bustling hub of festivals and culture it was voted "Most Charming" by the NY Times, "Friendliest" city by The US Travel & Leisure Magazine, "Europe's Micro City of the Year" by The Financial Times and the European Capital of Culture for 2020.
Applications are being taken on a rolling basis. Please see link below and circulate to any parties you feel may be interested in applying..
MSc in Microscopy & Imaging http://www.nuigalway.ie/microscopy-imaging/
Many thanks
Dr Kerry Thompson
Kerry Thompson PhD, Lecturer, Anatomy, School of Medicine, NUI Galway. P: +353(0)91495704
Secretary, Microscopy Society of Ireland - www.microscopy.ie Fellow, Outreach & Education Committee, Royal Microscopical Society - www.rms.org.uk
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both stacie-at-ems-secure.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Biological TEM Workshop, Personal Short Courses and more
Message: The EMS Microscopy Academy is pleased to announce the Biological TEM Workshop will be held November 7-9.
This course offers a complete picture of proper chemical processing of biological samples for TEM observation, teaching both theory and hands-on preparation using buffers, fixatives, and other solutions. Participants are encouraged to bring their own samples to get expert advice on the work they need to do back in their own lab.
Additional classes are forming for: Pharmaceutical Microscopy X-Ray Microanalysis Cryosectioning/ImmunoGold and more!
Don't see the topic you're interested in? Fill out our online form to request a topic, or schedule a 1-2 day Personal Short Course! Equipment Demos are also available for you to try before you buy.
For more information, visit our website at www.emsdiasum.com and follow the link to EMS Microscopy Academy to learn more!
Thanks, Stacie Kirsch
Login Host: 73.165.127.32 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ravi.thakkar369-at-gmail.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: ravi.thakkar369-at-gmail.com Name: Ravi
Title-Subject: [Filtered] Elemental analysis by SEM-EDS.
Message: Hi listeners, I have a few questions for EDS analysis using Electron microscope. I did one soil sample for one user, the composition of soil was unknown. So, they did SEM-EDS to know the composition of sample. 1. What's the difference between weight% & atomic% value in EDS report?
The answer to your stated question is simple. The answer to your unstated question is more complicated.
Mass fraction or weight fraction is just that. How many grams of that element would you find in 100 grams of samples. Atomic fraction converts that over to moles so you might determine stoichiometry and the formula, if appropriate. For example, pyrite should be 46.6 wt% Fe and 53.4 wt% S. If convert that to moles, you would find it is 33.3 at% Fe and 66.7 at% S which tells you there are two atoms of S for every atom of Fe. The formula is FeS2.
Now the unstated questions should be "Was the analysis done properly?" and "How accurate was the analysis?" The analysis was probably done on a rough powder preparation. How well did that represent the original sample? What effect did that have on the accuracy of the analysis? The best analyses are from flat, homogenous samples. I don't suppose the soil was only one phase. How did you handle oxygen in the sample; how well does your detector measure oxygen?
In short, the analysis will give a ballpark figure at best. I would not necessarily expect the same answer from two different preparations of the same sample. Caution your user about pushing the results too far.
Warren Straszheim
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Friday, July 14, 2017 8:46 AM To: Straszheim, Warren E [BIOTC]
X-from: ravi.thakkar369-at-gmail.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ravi.thakkar369-at-gmail.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: ravi.thakkar369-at-gmail.com Name: Ravi
Title-Subject: [Filtered] Elemental analysis by SEM-EDS.
Message: Hi listeners, I have a few questions for EDS analysis using Electron microscope. I did one soil sample for one user, the composition of soil was unknown. So, they did SEM-EDS to know the composition of sample. 1. What's the difference between weight% & atomic% value in EDS report?
From husechristena45-at-gmail.com Sun Jul 16 00:33:54 2017 Return-Path: {husechristena45-at-gmail.com} Received: from gmail.com (mail.dongnamdc.com [222.97.39.100] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v6G5Xp5h006986 for {microscopylistserverarchive6-at-microscopy.com} ; Sun, 16 Jul 2017 00:33:53 -0500 Message-ID: {996F56A8.A13A8D9C-at-gmail.com}
X-from: Ferenc Molnar {ferenc.l.molnar-at-googlemail.com}
X-from: Murowchick, James {MurowchickJ-at-umkc.edu} To: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com}
X-from: ravi.thakkar369-at-gmail.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ravi.thakkar369-at-gmail.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: ravi.thakkar369-at-gmail.com Name: Ravi
Title-Subject: [Filtered] Elemental analysis by SEM-EDS.
Message: Hi listeners, I have a few questions for EDS analysis using Electron microscope. I did one soil sample for one user, the composition of soil was unknown. So, they did SEM-EDS to know the composition of sample. 1. What's the difference between weight% & atomic% value in EDS report?
X-from: Madary, Joseph N (Nick) CIV DHA NCR MEDICAL DIR JPC (US) {joseph.n.madary.civ-at-mail.mil} To: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com}
Please note that for an EDS analysis you also have the option to normalize the results (so that the sum of the element you are looking for is always 100%).
Regards, Stefano
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: July 14, 2017 7:09 AM To: Stefano Rubino {stefano-at-soquelec.com}
X-from: ravi.thakkar369-at-gmail.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ravi.thakkar369-at-gmail.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: ravi.thakkar369-at-gmail.com Name: Ravi
Title-Subject: [Filtered] Elemental analysis by SEM-EDS.
Message: Hi listeners, I have a few questions for EDS analysis using Electron microscope. I did one soil sample for one user, the composition of soil was unknown. So, they did SEM-EDS to know the composition of sample. 1. What's the difference between weight% & atomic% value in EDS report?
Please note that for an EDS analysis you also have the option to normalize the results (so that the sum of the element you are looking for is always 100%).
Regards, Stefano
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: July 14, 2017 7:09 AM To: Stefano Rubino {stefano-at-soquelec.com}
X-from: ravi.thakkar369-at-gmail.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ravi.thakkar369-at-gmail.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: ravi.thakkar369-at-gmail.com Name: Ravi
Title-Subject: [Filtered] Elemental analysis by SEM-EDS.
Message: Hi listeners, I have a few questions for EDS analysis using Electron microscope. I did one soil sample for one user, the composition of soil was unknown. So, they did SEM-EDS to know the composition of sample. 1. What's the difference between weight% & atomic% value in EDS report?
X-from: Murowchick, James {MurowchickJ-at-umkc.edu}
Ravi, For individual mineral grain analyses, the atomic % is useful for identifying some minerals based on their stoichiometry since it gives the number of atoms of a particular element per 100 atoms total. Calcite, CaCO3 has 5 atoms, so it has 20 at. % Ca, 20 at % C, and 60 at. % O. Weight % is the mass of each element measured per the total mass. Calcite has molecular mass of (40.08 + 12.011+3*15.999) = 100.088g/mol, so it has 40.08/100.088 % Ca = 40.04 wt % Ca, and so on. For a soil or other mixture, the EDS spectrum should be collected over a large enough area that the results are representative of the bulk composition. Atomic % might not be very useful unless the numbers of each element are needed, but such results are often given in terms of wt %. Conversion from elemental wt % to atomic % or wt % as oxides (common for rock analyses) is relatively straightforward and described on most mineralogy textbooks.
I hope this helps.
Jim Dr. James B. Murowchick Professor, Geochemistry & Mineralogy Principal Graduate Advisor & IPhD Coordinator, Geosciences Department of Geosciences University of Missouri-Kansas City 420 Flarsheim Hall 5110 Rockhill Road Kansas City, MO 64110 Office: 816 235-2979 Department Office: 816 235-1334 Fax: 816 235-5535 murowchickj-at-umkc.edu
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Friday, July 14, 2017 9:02 AM To: Murowchick, James {MurowchickJ-at-umkc.edu}
X-from: ravi.thakkar369-at-gmail.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ravi.thakkar369-at-gmail.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: ravi.thakkar369-at-gmail.com Name: Ravi
Title-Subject: [Filtered] Elemental analysis by SEM-EDS.
Message: Hi listeners, I have a few questions for EDS analysis using Electron microscope. I did one soil sample for one user, the composition of soil was unknown. So, they did SEM-EDS to know the composition of sample. 1. What's the difference between weight% & atomic% value in EDS report?
The basic difference between wt% and at% in the EDS report the way the software calculates the composition. In soil, there are several Si-, Al-, and Fe- (hydr)oxide phases in its composition. So, be careful with the EDS quantification report, as you cannot quantify light elements (Z {11) by using EDS. There's always delocalized electrons contributing to the X-ray emission for light elements. For oxides, it's better to quantify oxygen in such samples indirectly, by running a quantification model that takes into account the charge balance. Regarding the w% of at%, it's up to you.
Cheers,
On Fri, Jul 14, 2017 at 11:06 AM, {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote:
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver On-Line {http://www.microscopy.com/MicroscopyListserver On-Line} Help http://www.microscopy.com/MicroscopyListserver/FAQ.html {http://www.microscopy.com/MicroscopyListserver/FAQ.html} ----------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html {http://microscopy.com/MLFormMail.html} --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ravi.thakkar369-at-gmail.com {mailto:ravi.thakkar369-at-gmail.com} as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: ravi.thakkar369-at-gmail.com {mailto:ravi.thakkar369-at-gmail.com} Name: Ravi
Title-Subject: [Filtered] Elemental analysis by SEM-EDS.
Message: Hi listeners, I have a few questions for EDS analysis using Electron microscope. I did one soil sample for one user, the composition of soil was unknown. So, they did SEM-EDS to know the composition of sample. 1. What's the difference between weight% & atomic% value in EDS report?
-- Erico Freitas Físico - Centro de Microscopia da UFMG Currículo Lattes: *http://lattes.cnpq.br/8786127123101199* {https://wwws.cnpq.br/cvlattesweb/PKG_MENU.menu?f_cod=DE6B009EAB5F41052FDE9CDAAECDEB36#}
From medinaluca1-at-gmail.com Tue Jul 18 04:00:29 2017 Return-Path: {medinaluca1-at-gmail.com} Received: from gmail.com ([182.16.3.90]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v6I90R4p002187 for {microscopylistserverarchive6-at-microscopy.com} ; Tue, 18 Jul 2017 04:00:28 -0500 Message-ID: {4F08F003.F69AC153-at-gmail.com}
X-from: kuryu-at-rockefeller.edu
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both kuryu-at-rockefeller.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Organization: The Rockefeller University, Electron Microscopy Resource Center
Title-Subject: [Filtered] Assistant Position available
Message: Dear List,
The Rockefeller University, New York, NY, seeks outstanding candidates for Electron Microscopy Resource Center (EMRC). The EMRC provides state of the art electron microscopy support for analysis of a wide variety of biological samples, including virus, bacteria, insects, animal tissue as well as cultured cell and isolated cellular components for structural analyses or immuno-electron microscopy. The EMRC is equipped with two TEMs and one SEM as well as a high pressure freezing and a free substitution unit. Please visit our website for more detail. (http://www.rockefeller.edu/emrc/)
Job Title: Electron Microscopists: Research Support Assistant Employment status: Full time
Department Description: The Electron Microscopy Resource Center (EMRC) is one of the Universitys scientific core facilities, providing expert electron microscopy services and training to researchers for The Rockefeller University and neighboring institutions. The EMRC provides state- of-the-art electron microscopy support for analysis of a wide variety of biological samples, including virus, bacteria, insects, animal tissue as well as cultured cell and isolated cellular components. It conducts structural analyses, immuno-electron microscopy, CLEM, SEM serial block face imaging, montage, and tomography. The EMRC is equipped with three TEMs and two SEM as well as a high pressure freezing and other ambient and cryo EM sample preparation devices. Job Responsibilities: Will support the Electron Microscopy Resource Center's (EMRC) daily operations, including bench work, sample preparation for transmission and scanning electron microscopy and maintenance of the center. Will be responsible for conventional sample preparation, including processing samples, cutting ultrathin sections, data collection, ordering and receiving supplies, and managing chemical waste compliance and administrative support for office duties. Job Requirements: Bachelor's degree in science required. Educational emphasis in biology, cell biology, bioengineering or a related field preferred. Must have strong communication skills, ability to work as part of a team, and flexibility to interact with a diverse group of researchers. Must be detail-oriented, focused, highly motivated, and able to work in a team atmosphere for general lab duties, as well as a mentored atmosphere for wide variety research projects. EM hands on experience preferred, but not required. Competency in use of computers is plus, including functional command of Word, Excel, PowerPoint, and Photoshop.
Please visit the following site to submit your application: Job: IRC20260
The Rockefeller University is an Equal Opportunity Employer with a policy that forbids discrimination in employment (which includes hiring, terms and conditions, promotion, and termination) on the basis of race, color, religion, sex, age, national origin, citizenship status, marital status, sexual orientation, military status, veteran status, or disability. The Administration has an Affirmative Action Program to increase the employment of women and members of protected classes in all areas of the University's activities.
Login Host: 149.86.181.159 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both semy66-at-yahoo.fr as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: semy66-at-yahoo.fr Name: Sami ZEKRI
Organization: TEM Lab. Faculty of Medicine- Tunis- Tunisia
Title-Subject: [Filtered] JEOL JEM 1010
Message: Hi every one,
I'm looking for a refurbished / old electronic " Panel ITF PB " card for JEOL JEM 1010 microscope.
Thanks
Login Host: 41.224.185.153 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both gary-at-cermetmaterials.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: gary-at-cermetmaterials.com Name: Gary Castelow
Organization: Cermet Materials
Title-Subject: [Filtered] JEOL 840A
Message: Hello all! I got some good help from here before so we decided to try again. We recently had a power blip/surge/loss? last Friday. We noticed late Monday afternoon our SEM was shut down. It would not turn back on with the key, so we checked the power box in the other room and the reset was tripped. We flipped it back on the tried the key again and it fired right up...for about 25-30secs. In the manual it says after about 30 secs the DP power supply will turn on. I reset the instrument and tried to fire it up again and watched the light panel, sure enough as soon as the DP light turns on the SEM shuts down. Bad power supply? Maybe shorted or fried? If so where is this power supply and how could I check it in house? Any other ideas of what it could be?
Login Host: 173.161.208.9 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
Did you check all the fuses at the power supply (the big yelloish block) ?
If you suspect the heater plate of the diffusion pump, take away the panels, unplug the heater and measure the resistance. I think it should be ca. 30 - 50 ohm.
When the SEM shuts down at start of DP heating, you might have a faulty heater plate. You will need to contact Jeol to get a fitting one (ca. 400 ...).
You can try unplug the heater and start the SEM. If it starts then you know; but: it will switch off later since the thermo-switch at the DP will not close after a certain time (normally 20 minutes)
What can also be a reason for the Jeol to switch off is:
- not reaching the pre-vacuum trip point (check your pre-vac pump suction pressure), faulty vacuum lines (I once had a leak just in between the vibration dampening weight...)
- no sufficient air pressure at the SEM (4 bar...)
Best,
Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Am 19.07.17 um 15:16 schrieb microscopy.listserver-at-gmail.com: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: gary-at-cermetmaterials.com } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } gary-at-cermetmaterials.com as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: gary-at-cermetmaterials.com Name: Gary Castelow } } Organization: Cermet Materials } } Title-Subject: [Filtered] JEOL 840A } } Message: Hello all! I got some good help from here before so we decided to try again. We recently } had a power blip/surge/loss? last Friday. We noticed late Monday afternoon our SEM was shut down. } It would not turn back on with the key, so we checked the power box in the other room and the reset } was tripped. We flipped it back on the tried the key again and it fired right up...for about } 25-30secs. In the manual it says after about 30 secs the DP power supply will turn on. I reset the } instrument and tried to fire it up again and watched the light panel, sure enough as soon as the DP } light turns on the SEM shuts down. Bad power supply? Maybe shorted or fried? If so where is this } power supply and how could I check it in house? Any other ideas of what it could be? } } Login Host: 173.161.208.9 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 9, 52 -- From microscopy.listserver-at-gmail.com Wed Jul 19 07:53:58 2017 } 9, 52 -- Received: from mail-io0-f175.google.com (mail-io0-f175.google.com [209.85.223.175]) } 9, 52 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v6JCrwmQ031894 } 9, 52 -- for {microscopy-at-microscopy.com} ; Wed, 19 Jul 2017 07:53:58 -0500 } 9, 52 -- Received: by mail-io0-f175.google.com with SMTP id g13so1487195ioj.5 } 9, 52 -- for {microscopy-at-microscopy.com} ; Wed, 19 Jul 2017 05:54:08 -0700 (PDT) } 9, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 9, 52 -- d=gmail.com; s=20161025; } 9, 52 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 9, 52 -- :in-reply-to:content-language:content-transfer-encoding; } 9, 52 -- bh=cDTPbCAN0xwTyK6ibCXQ/kI+3E3YDAfOMcsRKLHXH+8=; } 9, 52 -- b=uie8e4bpDaMkHsXhgsEQK/Z2oD/jw99+InVjR/XNSQBTb1PKUZUVhh4la7AWaN56K1 } 9, 52 -- kygfJcKhP43/Wo+bKIdX3lNdSE8dpQQuJnun19llvYrJ7EM4qIviONXLQ2I5vw4yHClX } 9, 52 -- WVttfAyA8voQZu1XlJqKvopBcBsQ+HSle/s8hTPRY7e6HBpivlnpzr1FX3zcy2SIqXf7 } 9, 52 -- KlAvClT6UD8VpsrlfOWsuKgo7YVVUQ7Ij8WzcTGF2e9sbHhGWPSabetVH/58VRxCte3w } 9, 52 -- TK7M81HNDgbtAAqdX1Noy/udLlskErAJK4rcf7xvmM5/knlUNBXdJ0UHeuC1zTo3oWNa } 9, 52 -- nWLw== } 9, 52 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 9, 52 -- d=1e100.net; s=20161025; } 9, 52 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 9, 52 -- :user-agent:mime-version:in-reply-to:content-language } 9, 52 -- :content-transfer-encoding; } 9, 52 -- bh=cDTPbCAN0xwTyK6ibCXQ/kI+3E3YDAfOMcsRKLHXH+8=; } 9, 52 -- b=qZcYNz0V0KLbSjanGNIbJj67IwCCWAOSOTioGipZ5im/fpHFKCDEDs6MgdLBb8KERH } 9, 52 -- QHn9+Ll7hf51iseHcF2Ckgz+12/SuVbHs9+rmWJCa+yS/vSoTTR+rivnzblLfHD78YPb } 9, 52 -- sfqxfm8jHKMebJc6jK/SUDzDmmqskdTbMFNZMeF/rAjANaeKr89wqomByMjiXDQ6ouat } 9, 52 -- Lgig/JKshUzjyr9tvGsMUXaAkvQioe0V2CrGVucrStrhlha7IsG5qGomAG9nuK/z3Tjt } 9, 52 -- QPGWugWKgjqGJxMXbh9SWra1AsLVgIqFk0v7vlnnbATp4zhkMIXY8e4Qdaxlm49qJpFL } 9, 52 -- s/Ig== } 9, 52 -- X-Gm-Message-State: AIVw110V8CubwzCeec+r39rHAk1ypXS+rZDxw4BztFw7abgyn8pHnRXX } 9, 52 -- by1MR4iGtTxyI+PxyKI= } 9, 52 -- X-Received: by 10.107.132.148 with SMTP id o20mr450905ioi.296.1500468848262; } 9, 52 -- Wed, 19 Jul 2017 05:54:08 -0700 (PDT) } 9, 52 -- Received: from anlvpn001.nst.anl.gov ([130.202.235.1]) } 9, 52 -- by smtp.googlemail.com with ESMTPSA id b24sm2816818iod.11.2017.07.19.05.54.06 } 9, 52 -- for {microscopy-at-microscopy.com} } 9, 52 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 9, 52 -- Wed, 19 Jul 2017 05:54:07 -0700 (PDT) } 9, 52 -- Subject: viaWWW:JEOL 840A help needed } 9, 52 -- References: {201707191114.v6JBEMRi029854-at-microscopy.com} } 9, 52 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 9, 52 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 9, 52 -- X-Forwarded-Message-Id: {201707191114.v6JBEMRi029854-at-microscopy.com} } 9, 52 -- Message-ID: {7159ef8a-117d-61e7-e984-a6823e11f2d8-at-gmail.com} } 9, 52 -- Date: Wed, 19 Jul 2017 07:54:06 -0500 } 9, 52 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) } 9, 52 -- Gecko/20100101 Thunderbird/52.2.1 } 9, 52 -- MIME-Version: 1.0 } 9, 52 -- In-Reply-To: {201707191114.v6JBEMRi029854-at-microscopy.com} } 9, 52 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 9, 52 -- Content-Language: en-US } 9, 52 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
==============================Original Headers============================== 18, 30 -- From diller-at-stefan-diller.com Wed Jul 19 11:16:06 2017 18, 30 -- Received: from mailout020.rox.net (mailout020.rox.net [212.63.85.220]) 18, 30 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v6JGG54H029746 18, 30 -- for {microscopy-at-microscopy.com} ; Wed, 19 Jul 2017 11:16:06 -0500 18, 30 -- Received: from localhost ([127.0.0.1]) 18, 30 -- by mailout02.rox.net with esmtp (Exim 4.80) 18, 30 -- (envelope-from {diller-at-stefan-diller.com} ) 18, 30 -- id 1dXre4-000DPm-0w; Wed, 19 Jul 2017 18:16:16 +0200 18, 30 -- Received: from p5dcb4fae.dip0.t-ipconnect.de ([93.203.79.174] helo=mac-pro.local) 18, 30 -- by mailout02.rox.net with esmtpsa (TLSv1.2:DHE-RSA-AES128-SHA:128) 18, 30 -- (Exim 4.80) 18, 30 -- (envelope-from {diller-at-stefan-diller.com} ) 18, 30 -- id 1dXre3-000DPe-SM; Wed, 19 Jul 2017 18:16:15 +0200 18, 30 -- Subject: Re: [Microscopy] viaWWW:JEOL 840A help needed 18, 30 -- To: "Microscopy-at-microscopy.com" {microscopy-at-microscopy.com} , 18, 30 -- gary-at-cermetmaterials.com 18, 30 -- References: {201707191316.v6JDGOx1021675-at-microscopy.com} 18, 30 -- From: stefan diller {diller-at-stefan-diller.com} 18, 30 -- Message-ID: {4f3c9b08-0143-2d2d-7cab-9c07999d4a7c-at-stefan-diller.com} 18, 30 -- Date: Wed, 19 Jul 2017 18:16:14 +0200 18, 30 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) 18, 30 -- Gecko/20100101 Thunderbird/52.2.1 18, 30 -- MIME-Version: 1.0 18, 30 -- In-Reply-To: {201707191316.v6JDGOx1021675-at-microscopy.com} 18, 30 -- Content-Type: text/plain; charset=windows-1252; format=flowed 18, 30 -- Content-Language: en-GB 18, 30 -- X-Envelope-From: {diller-at-stefan-diller.com} 18, 30 -- X-Scanned-By: rockenstein AG 18, 30 -- Content-Transfer-Encoding: 8bit 18, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v6JGG54H029746 ==============================End of - Headers==============================
Mississippi State University is seeking a Research Associate - Microscopy
Mississippi State University's (MSU), Institute for Imaging & Analytical Technologies (I2AT) is a university level research institute and core facility, meeting MSU's missions in research, education, and service. In order to properly serve the needs of the life-sciences and engineering research community, the I2AT requires a Research Associate to assist with diverse applications in biological-sample preparation, imaging, characterization and research. This individual will function at the professional level and will collaborate with researchers involved in experimental design, implementation, and analysis of a wide variety of life-science samples (e.g. micro-organisms in culture, in tissues and bio-films plus animal and plant tissues).
ESSENTIAL DUTIES & RESPONSIBILITIES The individual to be hired will be expected to have significant experience in biological materials sample preparation, imaging, and characterization. In particular, we are seeking an individual with experience in light, electron (both scanning and transmission), and confocal laser scanning microscopies. Preference will be given to individuals with experience with these techniques as they relate to biological-materials research.
MINIMUM QUALIFICATIONS The successful candidate must have, at a minimum, a MS in a life-sciences related field with emphasis and experience in biological research to include sample preparation for both scanning and transmission electron microscopies, as well as imaging and characterization.
Strong communication and writing skills and the ability to function effectively in a team environment is required. As an I2AT Research Associate, this person will have the opportunity to publish collaboratively and independently in his/her area of expertise. The Research Associate will also be given the opportunity to be involved in grant proposal development and implementation. The I2AT Research Associate will work with the Director to implement performance/career development planning and review.
PREFERRED QUALIFICATIONS * PhD in Life-Sciences field, minimum 2 years of experience in biological electron microscopy * MS in Life-Sciences field, 3-5 year of experience in biological electron microscopy * Cryo sample preparation * Cryo electron microscopy experience
If interested click or copy and paste this link: http://explore.msujobs.msstate.edu/cw/en-us/job/495702/research-associate-iiiiii-or-senior
==============================Original Headers============================== 9, 32 -- From zrowland-at-i2at.msstate.edu Wed Jul 19 11:29:37 2017 9, 32 -- Received: from chokecherry.its.msstate.edu (chokecherry.its.msstate.edu [130.18.2.120]) 9, 32 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v6JGTaXk010855 9, 32 -- for {Microscopy-at-Microscopy.Com} ; Wed, 19 Jul 2017 11:29:37 -0500 9, 32 -- Received: from mail01.ad.msstate.edu (mail01.ad.msstate.edu [130.18.230.60]) 9, 32 -- by chokecherry.its.msstate.edu (8.13.8/8.13.8) with ESMTP id v6JGTlbj009008 9, 32 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=FAIL) 9, 32 -- for {Microscopy-at-Microscopy.Com} ; Wed, 19 Jul 2017 11:29:47 -0500 9, 32 -- X-Sender: {} 9, 32 -- Received: from MAIL01.ad.msstate.edu (2620:0:1a30:1230::60) by 9, 32 -- mail01.ad.msstate.edu (2620:0:1a30:1230::60) with Microsoft SMTP Server (TLS) 9, 32 -- id 15.0.1236.3; Wed, 19 Jul 2017 11:29:47 -0500 9, 32 -- Received: from MAIL01.ad.msstate.edu ([fe80::85cd:146c:55d4:e430]) by 9, 32 -- mail01.ad.msstate.edu ([fe80::85cd:146c:55d4:e430%14]) with mapi id 9, 32 -- 15.00.1236.000; Wed, 19 Jul 2017 11:29:47 -0500 9, 32 -- From: "Rowland, Zach" {zrowland-at-i2at.msstate.edu} 9, 32 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com} 9, 32 -- Subject: Job Opportunity - Mississippi State University 9, 32 -- Thread-Topic: Job Opportunity - Mississippi State University 9, 32 -- Thread-Index: AdMAqn2ZlxUcNoo0TU2Osu/NyKu/Yw== 9, 32 -- Date: Wed, 19 Jul 2017 16:29:47 +0000 9, 32 -- Message-ID: {c8cac93df9c748ff9adeddd70e5dceee-at-mail01.ad.msstate.edu} 9, 32 -- Accept-Language: en-US 9, 32 -- Content-Language: en-US 9, 32 -- X-MS-Has-Attach: 9, 32 -- X-MS-TNEF-Correlator: 9, 32 -- x-ms-exchange-transport-fromentityheader: Hosted 9, 32 -- x-originating-ip: [130.18.230.93] 9, 32 -- Content-Type: text/plain; charset="us-ascii" 9, 32 -- MIME-Version: 1.0 9, 32 -- Content-Transfer-Encoding: 8bit 9, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v6JGTaXk010855 ==============================End of - Headers==============================
From rosaronald70-at-gmail.com Wed Jul 19 19:12:35 2017 Return-Path: {rosaronald70-at-gmail.com} Received: from gmail.com (mail.dongnamdc.com [222.97.39.100] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v6K0CWQE024679 for {microscopylistserverarchive6-at-microscopy.com} ; Wed, 19 Jul 2017 19:12:34 -0500 Message-ID: {076E67CA.21355118-at-gmail.com}
Hello, I was wondering if there are any labs out there that use molecular sieved 200 proof ethanol for dehydrating TEM samples? Do you prewash them in ethanol and then bake out before use? I used to do this but switched to the pint sized bottles (opening up a new one for each new exp). The problem of course is generating too many bottles of unused ethanol. All comments are welcome. If you do use it please forward a catalog number and vendor.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both kattymansouri-at-gmail.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Message: Hello, We have an old Hummer X sputter coater/plasma cleaner that needs to be repaired. Does anyone know or know of somebody that could do that for us? We are at Duke University, and prefer someone local. Thanks
Katayoun
Login Host: 152.16.15.224 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
To: microscopy.listserver-at-gmail.com, gary-at-cermetmaterials.com
Hello Gary, It sounds like DP heater problem. There is two diffusion pumps under the column of your SEM, a small one and a bigger one ; The heaters are at the bottom and are connected to 200V by a ceramic plug. Shutdown the SEM, unplug the ceramic plug and check if the two pins are still isolated from the ground. If not, there is a short circuit and you may buy another heater. Such problem appears often when the SEM shutdown and when the water still flow in the pump hoses. Humidity of the room and temperature gap between water and air act together to condensate water on the body of the pumps. This water fall on the heater... Hope this is help.
Nicolas STEPHANT
Université de Nantes Institut Jean Rouxel Service de microscopie électronique à balayage et microanalyse 2 rue de la Houssinière BP 92208 44322 Nantes cédex 3
"Le monde n'existe que pour autant que nous sommes capables d'en produire une image" C.G Jung
Le 19/07/2017 à 15:07, microscopy.listserver-at-gmail.com a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: gary-at-cermetmaterials.com } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } gary-at-cermetmaterials.com as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: gary-at-cermetmaterials.com Name: Gary Castelow } } Organization: Cermet Materials } } Title-Subject: [Filtered] JEOL 840A } } Message: Hello all! I got some good help from here before so we decided to try again. We recently } had a power blip/surge/loss? last Friday. We noticed late Monday afternoon our SEM was shut down. } It would not turn back on with the key, so we checked the power box in the other room and the reset } was tripped. We flipped it back on the tried the key again and it fired right up...for about } 25-30secs. In the manual it says after about 30 secs the DP power supply will turn on. I reset the } instrument and tried to fire it up again and watched the light panel, sure enough as soon as the DP } light turns on the SEM shuts down. Bad power supply? Maybe shorted or fried? If so where is this } power supply and how could I check it in house? Any other ideas of what it could be? } } Login Host: 173.161.208.9 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 9, 52 -- From microscopy.listserver-at-gmail.com Wed Jul 19 07:53:58 2017 } 9, 52 -- Received: from mail-io0-f175.google.com (mail-io0-f175.google.com [209.85.223.175]) } 9, 52 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v6JCrwmQ031894 } 9, 52 -- for {microscopy-at-microscopy.com} ; Wed, 19 Jul 2017 07:53:58 -0500 } 9, 52 -- Received: by mail-io0-f175.google.com with SMTP id g13so1487195ioj.5 } 9, 52 -- for {microscopy-at-microscopy.com} ; Wed, 19 Jul 2017 05:54:08 -0700 (PDT) } 9, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 9, 52 -- d=gmail.com; s=20161025; } 9, 52 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 9, 52 -- :in-reply-to:content-language:content-transfer-encoding; } 9, 52 -- bh=cDTPbCAN0xwTyK6ibCXQ/kI+3E3YDAfOMcsRKLHXH+8=; } 9, 52 -- b=uie8e4bpDaMkHsXhgsEQK/Z2oD/jw99+InVjR/XNSQBTb1PKUZUVhh4la7AWaN56K1 } 9, 52 -- kygfJcKhP43/Wo+bKIdX3lNdSE8dpQQuJnun19llvYrJ7EM4qIviONXLQ2I5vw4yHClX } 9, 52 -- WVttfAyA8voQZu1XlJqKvopBcBsQ+HSle/s8hTPRY7e6HBpivlnpzr1FX3zcy2SIqXf7 } 9, 52 -- KlAvClT6UD8VpsrlfOWsuKgo7YVVUQ7Ij8WzcTGF2e9sbHhGWPSabetVH/58VRxCte3w } 9, 52 -- TK7M81HNDgbtAAqdX1Noy/udLlskErAJK4rcf7xvmM5/knlUNBXdJ0UHeuC1zTo3oWNa } 9, 52 -- nWLw== } 9, 52 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 9, 52 -- d=1e100.net; s=20161025; } 9, 52 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 9, 52 -- :user-agent:mime-version:in-reply-to:content-language } 9, 52 -- :content-transfer-encoding; } 9, 52 -- bh=cDTPbCAN0xwTyK6ibCXQ/kI+3E3YDAfOMcsRKLHXH+8=; } 9, 52 -- b=qZcYNz0V0KLbSjanGNIbJj67IwCCWAOSOTioGipZ5im/fpHFKCDEDs6MgdLBb8KERH } 9, 52 -- QHn9+Ll7hf51iseHcF2Ckgz+12/SuVbHs9+rmWJCa+yS/vSoTTR+rivnzblLfHD78YPb } 9, 52 -- sfqxfm8jHKMebJc6jK/SUDzDmmqskdTbMFNZMeF/rAjANaeKr89wqomByMjiXDQ6ouat } 9, 52 -- Lgig/JKshUzjyr9tvGsMUXaAkvQioe0V2CrGVucrStrhlha7IsG5qGomAG9nuK/z3Tjt } 9, 52 -- QPGWugWKgjqGJxMXbh9SWra1AsLVgIqFk0v7vlnnbATp4zhkMIXY8e4Qdaxlm49qJpFL } 9, 52 -- s/Ig== } 9, 52 -- X-Gm-Message-State: AIVw110V8CubwzCeec+r39rHAk1ypXS+rZDxw4BztFw7abgyn8pHnRXX } 9, 52 -- by1MR4iGtTxyI+PxyKI= } 9, 52 -- X-Received: by 10.107.132.148 with SMTP id o20mr450905ioi.296.1500468848262; } 9, 52 -- Wed, 19 Jul 2017 05:54:08 -0700 (PDT) } 9, 52 -- Received: from anlvpn001.nst.anl.gov ([130.202.235.1]) } 9, 52 -- by smtp.googlemail.com with ESMTPSA id b24sm2816818iod.11.2017.07.19.05.54.06 } 9, 52 -- for {microscopy-at-microscopy.com} } 9, 52 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 9, 52 -- Wed, 19 Jul 2017 05:54:07 -0700 (PDT) } 9, 52 -- Subject: viaWWW:JEOL 840A help needed } 9, 52 -- References: {201707191114.v6JBEMRi029854-at-microscopy.com} } 9, 52 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 9, 52 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 9, 52 -- X-Forwarded-Message-Id: {201707191114.v6JBEMRi029854-at-microscopy.com} } 9, 52 -- Message-ID: {7159ef8a-117d-61e7-e984-a6823e11f2d8-at-gmail.com} } 9, 52 -- Date: Wed, 19 Jul 2017 07:54:06 -0500 } 9, 52 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) } 9, 52 -- Gecko/20100101 Thunderbird/52.2.1 } 9, 52 -- MIME-Version: 1.0 } 9, 52 -- In-Reply-To: {201707191114.v6JBEMRi029854-at-microscopy.com} } 9, 52 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 9, 52 -- Content-Language: en-US } 9, 52 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
X-from: Hank Beebe {HBeebe-at-rjleegroup.com} To: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com}
Gary, Check the indicators on the RP. Check the thermo stw on the DP
Hank Beebe Manager, Instrument Services RJ Lee Group
724.325.1776 Office 724.387.1826 Direct 412.897.8402 Mobile HBeebe-at-rjleegroup.com
Website | Lab Services | Shop | Pay My Bill
350 Hochberg Road | Monroeville, PA 15146
How are we doing? Take the Customer Survey.
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Wednesday, July 19, 2017 8:55 AM To: Hank Beebe {HBeebe-at-rjleegroup.com}
X-from: gary-at-cermetmaterials.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both gary-at-cermetmaterials.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: gary-at-cermetmaterials.com Name: Gary Castelow
Organization: Cermet Materials
Title-Subject: [Filtered] JEOL 840A
Message: Hello all! I got some good help from here before so we decided to try again. We recently had a power blip/surge/loss? last Friday. We noticed late Monday afternoon our SEM was shut down. It would not turn back on with the key, so we checked the power box in the other room and the reset was tripped. We flipped it back on the tried the key again and it fired right up...for about 25-30secs. In the manual it says after about 30 secs the DP power supply will turn on. I reset the instrument and tried to fire it up again and watched the light panel, sure enough as soon as the DP light turns on the SEM shuts down. Bad power supply? Maybe shorted or fried? If so where is this power supply and how could I check it in house? Any other ideas of what it could be?
Login Host: 173.161.208.9 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
==============================Original Headers============================== 9, 52 -- From microscopy.listserver-at-gmail.com Wed Jul 19 07:53:58 2017 9, 52 -- Received: from mail-io0-f175.google.com (mail-io0-f175.google.com [209.85.223.175]) 9, 52 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v6JCrwmQ031894 9, 52 -- for {microscopy-at-microscopy.com} ; Wed, 19 Jul 2017 07:53:58 -0500 9, 52 -- Received: by mail-io0-f175.google.com with SMTP id g13so1487195ioj.5 9, 52 -- for {microscopy-at-microscopy.com} ; Wed, 19 Jul 2017 05:54:08 -0700 (PDT) 9, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 9, 52 -- d=gmail.com; s=20161025; 9, 52 -- h=subject:references:to:from:message-id:date:user-agent:mime-version 9, 52 -- :in-reply-to:content-language:content-transfer-encoding; 9, 52 -- bh=cDTPbCAN0xwTyK6ibCXQ/kI+3E3YDAfOMcsRKLHXH+8=; 9, 52 -- b=uie8e4bpDaMkHsXhgsEQK/Z2oD/jw99+InVjR/XNSQBTb1PKUZUVhh4la7AWaN56K1 9, 52 -- kygfJcKhP43/Wo+bKIdX3lNdSE8dpQQuJnun19llvYrJ7EM4qIviONXLQ2I5vw4yHClX 9, 52 -- WVttfAyA8voQZu1XlJqKvopBcBsQ+HSle/s8hTPRY7e6HBpivlnpzr1FX3zcy2SIqXf7 9, 52 -- KlAvClT6UD8VpsrlfOWsuKgo7YVVUQ7Ij8WzcTGF2e9sbHhGWPSabetVH/58VRxCte3w 9, 52 -- TK7M81HNDgbtAAqdX1Noy/udLlskErAJK4rcf7xvmM5/knlUNBXdJ0UHeuC1zTo3oWNa 9, 52 -- nWLw== 9, 52 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 9, 52 -- d=1e100.net; s=20161025; 9, 52 -- h=x-gm-message-state:subject:references:to:from:message-id:date 9, 52 -- :user-agent:mime-version:in-reply-to:content-language 9, 52 -- :content-transfer-encoding; 9, 52 -- bh=cDTPbCAN0xwTyK6ibCXQ/kI+3E3YDAfOMcsRKLHXH+8=; 9, 52 -- b=qZcYNz0V0KLbSjanGNIbJj67IwCCWAOSOTioGipZ5im/fpHFKCDEDs6MgdLBb8KERH 9, 52 -- QHn9+Ll7hf51iseHcF2Ckgz+12/SuVbHs9+rmWJCa+yS/vSoTTR+rivnzblLfHD78YPb 9, 52 -- sfqxfm8jHKMebJc6jK/SUDzDmmqskdTbMFNZMeF/rAjANaeKr89wqomByMjiXDQ6ouat 9, 52 -- Lgig/JKshUzjyr9tvGsMUXaAkvQioe0V2CrGVucrStrhlha7IsG5qGomAG9nuK/z3Tjt 9, 52 -- QPGWugWKgjqGJxMXbh9SWra1AsLVgIqFk0v7vlnnbATp4zhkMIXY8e4Qdaxlm49qJpFL 9, 52 -- s/Ig== 9, 52 -- X-Gm-Message-State: AIVw110V8CubwzCeec+r39rHAk1ypXS+rZDxw4BztFw7abgyn8pHnRXX 9, 52 -- by1MR4iGtTxyI+PxyKI= 9, 52 -- X-Received: by 10.107.132.148 with SMTP id o20mr450905ioi.296.1500468848262; 9, 52 -- Wed, 19 Jul 2017 05:54:08 -0700 (PDT) 9, 52 -- Received: from anlvpn001.nst.anl.gov ([130.202.235.1]) 9, 52 -- by smtp.googlemail.com with ESMTPSA id b24sm2816818iod.11.2017.07.19.05.54.06 9, 52 -- for {microscopy-at-microscopy.com} 9, 52 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 9, 52 -- Wed, 19 Jul 2017 05:54:07 -0700 (PDT) 9, 52 -- Subject: viaWWW:JEOL 840A help needed 9, 52 -- References: {201707191114.v6JBEMRi029854-at-microscopy.com} 9, 52 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 9, 52 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} 9, 52 -- X-Forwarded-Message-Id: {201707191114.v6JBEMRi029854-at-microscopy.com} 9, 52 -- Message-ID: {7159ef8a-117d-61e7-e984-a6823e11f2d8-at-gmail.com} 9, 52 -- Date: Wed, 19 Jul 2017 07:54:06 -0500 9, 52 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) 9, 52 -- Gecko/20100101 Thunderbird/52.2.1 9, 52 -- MIME-Version: 1.0 9, 52 -- In-Reply-To: {201707191114.v6JBEMRi029854-at-microscopy.com} 9, 52 -- Content-Type: text/plain; charset=windows-1252; format=flowed 9, 52 -- Content-Language: en-US 9, 52 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers============================== The information contained in this e-mail message, together with any attachments thereto, is intended only for the personal and confidential use of the addressee[s] named above. The message and the attachments are or may be an attorney-client or other privileged or protected communication. If you are not the intended recipient of this message, or authorized to receive it for the intended recipient, you have received this message in error. You are not to review, use, disseminate, distribute or copy this message, any attachments thereto, or their contents. If you have received this message in error, please immediately notify us by return e-mail message, and delete the original message. Thank you for your cooperation.
I have used molecular sieve in 100% EtOH for years, it works well. I use mSorb sieves, and they are packaged to be used as received - that is, no pre-baking before use, nor do they need washing in ethanol before use. Which is good, because unless the ethanol is absolutely anhydrous, some of the water capacity of the sieve would be used up in the ethanol wash, and then you would have to pre-bake. Just watch the dust - be careful getting EtOH out of the bottle with sieve in it, or put the sieve in dialysis tubing.
I ordered these from Delta Absorbents, Cat #MS3AEDG05 in 5 gal pails (*much* cheaper than buying from lab supply or EM supply companies) and MSBI4A4801 for the blue indicating sieve (1 lb packets). The indicating sieve is *much* more expensive, so buy a separate packet and mix it 1:5 or 1:10 with the non-indicating sieve.
Phil ------------- Philip Oshel Imaging Facility Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 989 774-3576 office 989 774-7567 lab
Hello, I was wondering if there are any labs out there that use molecular sieved 200 proof ethanol for dehydrating TEM samples? Do you prewash them in ethanol and then bake out before use? I used to do this but switched to the pint sized bottles (opening up a new one for each new exp). The problem of course is generating too many bottles of unused ethanol. All comments are welcome. If you do use it please forward a catalog number and vendor.
Thank you, Michael Delannoy
------------- Philip Oshel Imaging Facility Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 989 774-3576 office 989 774-7567 lab
Hello, Many thanks to all your quick responses to my molecular sieve questions. I think we will give Phil Oshel's protocol a shot: Order a 5 1b sieve and 1 lb indicator sieve, mix 1:5. From Delta adsorbents. Ready to use no washing or baking required (I like that).
Residual molecular sieves will kill your diamond knife if it makes it into your block. Known problem with using this to dry ETOH.
Al Coritz Applications & Service Manager Electron Microscopy Sciences 1560 Industry Road Hatfield, PA 19440 acoritz-at-emsdiasum.com 800-523-5874
-----Original Message----- X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu] Sent: Thursday, July 20, 2017 11:35 AM To: Al Coritz {acoritz-at-emsdiasum.com}
Michael,
I have used molecular sieve in 100% EtOH for years, it works well. I use mSorb sieves, and they are packaged to be used as received - that is, no pre-baking before use, nor do they need washing in ethanol before use. Which is good, because unless the ethanol is absolutely anhydrous, some of the water capacity of the sieve would be used up in the ethanol wash, and then you would have to pre-bake. Just watch the dust - be careful getting EtOH out of the bottle with sieve in it, or put the sieve in dialysis tubing.
I ordered these from Delta Absorbents, Cat #MS3AEDG05 in 5 gal pails (*much* cheaper than buying from lab supply or EM supply companies) and MSBI4A4801 for the blue indicating sieve (1 lb packets). The indicating sieve is *much* more expensive, so buy a separate packet and mix it 1:5 or 1:10 with the non-indicating sieve.
Phil ------------- Philip Oshel Imaging Facility Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 989 774-3576 office 989 774-7567 lab
Hello, I was wondering if there are any labs out there that use molecular sieved 200 proof ethanol for dehydrating TEM samples? Do you prewash them in ethanol and then bake out before use? I used to do this but switched to the pint sized bottles (opening up a new one for each new exp). The problem of course is generating too many bottles of unused ethanol. All comments are welcome. If you do use it please forward a catalog number and vendor.
Thank you, Michael Delannoy
------------- Philip Oshel Imaging Facility Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 989 774-3576 office 989 774-7567 lab
Very true! Not a problem I’ve had, but then it’s just a matter of being careful to not stir up the sieve when in ethanol - or of putting the sieve in dialysis tubing.
Phil ------------- Philip Oshel Imaging Facility Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 989 774-3576 office 989 774-7567 lab
-----Original Message----- X-from: Al Coritz {acoritz-at-emsdiasum.com}
Michael,
I have used molecular sieve in 100% EtOH for years, it works well. I use mSorb sieves, and they are packaged to be used as received - that is, no pre-baking before use, nor do they need washing in ethanol before use. Which is good, because unless the ethanol is absolutely anhydrous, some of the water capacity of the sieve would be used up in the ethanol wash, and then you would have to pre-bake. Just watch the dust - be careful getting EtOH out of the bottle with sieve in it, or put the sieve in dialysis tubing.
I ordered these from Delta Absorbents, Cat #MS3AEDG05 in 5 gal pails (*much* cheaper than buying from lab supply or EM supply companies) and MSBI4A4801 for the blue indicating sieve (1 lb packets). The indicating sieve is *much* more expensive, so buy a separate packet and mix it 1:5 or 1:10 with the non-indicating sieve.
Phil ------------- Philip Oshel Imaging Facility Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 989 774-3576 office 989 774-7567 lab
Hello, I was wondering if there are any labs out there that use molecular sieved 200 proof ethanol for dehydrating TEM samples? Do you prewash them in ethanol and then bake out before use? I used to do this but switched to the pint sized bottles (opening up a new one for each new exp). The problem of course is generating too many bottles of unused ethanol. All comments are welcome. If you do use it please forward a catalog number and vendor.
Thank you, Michael Delannoy
------------- Philip Oshel Imaging Facility Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 989 774-3576 office 989 774-7567 lab
Most likely your DPs are full of water due to condensation from the cooling water. If you dry them out, they may work but most likely need replacing.
On Thu, Jul 20, 2017 at 9:49 AM, {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote:
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver On-Line {http://www.microscopy.com/MicroscopyListserver On-Line} Help http://www.microscopy.com/MicroscopyListserver/FAQ.html {http://www.microscopy.com/MicroscopyListserver/FAQ.html} ----------------------------------------------------------------------------
To: microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} , gary-at-cermetmaterials.com {mailto:gary-at-cermetmaterials.com}
Hello Gary, It sounds like DP heater problem. There is two diffusion pumps under the column of your SEM, a small one and a bigger one ; The heaters are at the bottom and are connected to 200V by a ceramic plug. Shutdown the SEM, unplug the ceramic plug and check if the two pins are still isolated from the ground. If not, there is a short circuit and you may buy another heater. Such problem appears often when the SEM shutdown and when the water still flow in the pump hoses. Humidity of the room and temperature gap between water and air act together to condensate water on the body of the pumps. This water fall on the heater... Hope this is help.
Nicolas STEPHANT
Université de Nantes Institut Jean Rouxel Service de microscopie électronique à balayage et microanalyse 2 rue de la Houssinière BP 92208 44322 Nantes cédex 3
"Le monde n'existe que pour autant que nous sommes capables d'en produire une image" C.G Jung
Le 19/07/2017 à 15:07, microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver {http://www.microscopy.com/MicroscopyListserver} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html {http://www.microscopy.com/MicroscopyListserver/FAQ.html} } ---------------------------------------------------------------------------- } } X-from: gary-at-cermetmaterials.com {mailto:gary-at-cermetmaterials.com} } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html {http://www.microscopy.com/MLFormMail.html} } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } gary-at-cermetmaterials.com {mailto:gary-at-cermetmaterials.com} as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: gary-at-cermetmaterials.com {mailto:gary-at-cermetmaterials.com} Name: Gary Castelow } } Organization: Cermet Materials } } Title-Subject: [Filtered] JEOL 840A } } Message: Hello all! I got some good help from here before so we decided to try again. We recently } had a power blip/surge/loss? last Friday. We noticed late Monday afternoon our SEM was shut down. } It would not turn back on with the key, so we checked the power box in the other room and the reset } was tripped. We flipped it back on the tried the key again and it fired right up...for about } 25-30secs. In the manual it says after about 30 secs the DP power supply will turn on. I reset the } instrument and tried to fire it up again and watched the light panel, sure enough as soon as the DP } light turns on the SEM shuts down. Bad power supply? Maybe shorted or fried? If so where is this } power supply and how could I check it in house? Any other ideas of what it could be? } } Login Host: 173.161.208.9 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 9, 52 -- From microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} Wed Jul 19 07:53:58 2017 } 9, 52 -- Received: from mail-io0-f175.google.com {http://mail-io0-f175.google.com} (mail-io0-f175.google.com {http://mail-io0-f175.google.com} [209.85.223.175]) } 9, 52 -- by microscopy.com {http://microscopy.com} (8.12.11.20060308/8.12.8) with ESMTP id v6JCrwmQ031894 } 9, 52 -- for {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } ; Wed, 19 Jul 2017 07:53:58 -0500 } 9, 52 -- Received: by mail-io0-f175.google.com {http://mail-io0-f175.google.com} with SMTP id g13so1487195ioj.5 } 9, 52 -- for {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } ; Wed, 19 Jul 2017 05:54:08 -0700 (PDT) } 9, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 9, 52 -- d=gmail.com {http://gmail.com} ; s=20161025; } 9, 52 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 9, 52 -- :in-reply-to:content-language:content-transfer-encoding; } 9, 52 -- bh=cDTPbCAN0xwTyK6ibCXQ/kI+3E3YDAfOMcsRKLHXH+8=; } 9, 52 -- b=uie8e4bpDaMkHsXhgsEQK/Z2oD/jw99+InVjR/XNSQBTb1PKUZUVhh4la7AWaN56K1 } 9, 52 -- kygfJcKhP43/Wo+bKIdX3lNdSE8dpQQuJnun19llvYrJ7EM4qIviONXLQ2I5vw4yHClX } 9, 52 -- WVttfAyA8voQZu1XlJqKvopBcBsQ+HSle/s8hTPRY7e6HBpivlnpzr1FX3zcy2SIqXf7 } 9, 52 -- KlAvClT6UD8VpsrlfOWsuKgo7YVVUQ7Ij8WzcTGF2e9sbHhGWPSabetVH/58VRxCte3w } 9, 52 -- TK7M81HNDgbtAAqdX1Noy/udLlskErAJK4rcf7xvmM5/knlUNBXdJ0UHeuC1zTo3oWNa } 9, 52 -- nWLw== } 9, 52 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 9, 52 -- d=1e100.net {http://1e100.net} ; s=20161025; } 9, 52 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 9, 52 -- :user-agent:mime-version:in-reply-to:content-language } 9, 52 -- :content-transfer-encoding; } 9, 52 -- bh=cDTPbCAN0xwTyK6ibCXQ/kI+3E3YDAfOMcsRKLHXH+8=; } 9, 52 -- b=qZcYNz0V0KLbSjanGNIbJj67IwCCWAOSOTioGipZ5im/fpHFKCDEDs6MgdLBb8KERH } 9, 52 -- QHn9+Ll7hf51iseHcF2Ckgz+12/SuVbHs9+rmWJCa+yS/vSoTTR+rivnzblLfHD78YPb } 9, 52 -- sfqxfm8jHKMebJc6jK/SUDzDmmqskdTbMFNZMeF/rAjANaeKr89wqomByMjiXDQ6ouat } 9, 52 -- Lgig/JKshUzjyr9tvGsMUXaAkvQioe0V2CrGVucrStrhlha7IsG5qGomAG9nuK/z3Tjt } 9, 52 -- QPGWugWKgjqGJxMXbh9SWra1AsLVgIqFk0v7vlnnbATp4zhkMIXY8e4Qdaxlm49qJpFL } 9, 52 -- s/Ig== } 9, 52 -- X-Gm-Message-State: AIVw110V8CubwzCeec+r39rHAk1ypXS+rZDxw4BztFw7abgyn8pHnRXX } 9, 52 -- by1MR4iGtTxyI+PxyKI= } 9, 52 -- X-Received: by 10.107.132.148 with SMTP id o20mr450905ioi.296.1500468848262; } 9, 52 -- Wed, 19 Jul 2017 05:54:08 -0700 (PDT) } 9, 52 -- Received: from anlvpn001.nst.anl.gov {http://anlvpn001.nst.anl.gov} ([130.202.235.1]) } 9, 52 -- by smtp.googlemail.com {http://smtp.googlemail.com} with ESMTPSA id b24sm2816818iod.11.2017.07.19.05.54.06 } 9, 52 -- for {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } } 9, 52 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 9, 52 -- Wed, 19 Jul 2017 05:54:07 -0700 (PDT) } 9, 52 -- Subject: viaWWW:JEOL 840A help needed } 9, 52 -- References: {201707191114.v6JBEMRi029854-at-microscopy.com {mailto:201707191114.v6JBEMRi029854-at-microscopy.com} } } 9, 52 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } } 9, 52 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } } 9, 52 -- X-Forwarded-Message-Id: {201707191114.v6JBEMRi029854-at-microscopy.com {mailto:201707191114.v6JBEMRi029854-at-microscopy.com} } } 9, 52 -- Message-ID: {7159ef8a-117d-61e7-e984-a6823e11f2d8-at-gmail.com {mailto:7159ef8a-117d-61e7-e984-a6823e11f2d8-at-gmail.com} } } 9, 52 -- Date: Wed, 19 Jul 2017 07:54:06 -0500 } 9, 52 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) } 9, 52 -- Gecko/20100101 Thunderbird/52.2.1 } 9, 52 -- MIME-Version: 1.0 } 9, 52 -- In-Reply-To: {201707191114.v6JBEMRi029854-at-microscopy.com {mailto:201707191114.v6JBEMRi029854-at-microscopy.com} } } 9, 52 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 9, 52 -- Content-Language: en-US } 9, 52 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both bassimn-at-mcmaster.ca as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: bassimn-at-mcmaster.ca Name: Nabil Bassim
Organization: McMaster University
Title-Subject: [Filtered] Postdoctoral Fellow/Research Associate Position Available
Message: The Bassim group at McMaster University is seeking a Research Associate or Postdoctoral Fellow in Microscopy
McMaster University has a strong electron microscopy effort, centered around the Canadian Centre for Electron Microscopy, which is a state-of-the-art national facility located on the campus of McMaster University. This position would leverage the individual's skills with electron microscopy methods in materials science to use advanced aberration-corrected instruments to research a variety of materials systems, including traditional metallurgical alloys, nanomaterials, and electronic thin films.
ESSENTIAL DUTIES & RESPONSIBILITIES The individual to be hired will be expected to have significant experience in materials characterization using transmission electron microscopy and such techniques as diffration-methods, TEM, STEM, EDS and EELS. In particular, we seek an individual with experience in using aberration-corrected (S)TEM methods, and with a versatile background.
MINIMUM QUALIFICATIONS The successful candidate must have a Ph.D in a materials-sciences related field with emphasis and experience in transmission electron microscopy, with experience in imaging, diffraction and spectroscopy. Strong communication and writing skills and the ability to function effectively in a team environment is required. This person will have the opportunity to publish collaboratively and independently in his/her area of expertise with the balance worked out depending on available funding and time. The Research Associate will also be given the opportunity to be involved in grant proposal development and implementation. PREFERRED QUALIFICATIONS * Aberration-corrected TEM experience * Sample preparation capabilities * Familiarity with metallurgical microstructures * willingness to mentor younger graduate students.
If you are interested, please email Prof. Nabil Bassim (bassimn-at-mcmaster.ca). If you are onsite at M&M in August, please mention this in your email of interest as there is an opportunity for a 1-1 interview in St. Louis.
Login Host: 130.113.74.83 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
X-from: David Strachan {d.strachan-at-beatson.gla.ac.uk} To: Jonathan Cherry {jpcherry.gtd-at-gmail.com} CC: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com}
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html {http://www.microscopy.com/MLFormMail.html} --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy bothD.Strachan-at-Beatson.gla.ac.uk {mailto:D.Strachan-at-Beatson.gla.ac.uk} as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email:D.Strachan-at-Beatson.gla.ac.uk {mailto:D.Strachan-at-Beatson.gla.ac.uk} Name: David Strachan
Organization: Beatson Institute for Cancer Research
Title-Subject: [Filtered] Alternatives to Essen BioScience Incucyte
Message: Dear List,
I was wondering if anyone has any thoughts on alternatives to the Essen Bioscience Incucyte system. Essentially we would like a system that can accommodate multi-well plates, image for up to one week, so probably placed inside an incubator or have its own specialised environmental control. Be capable of phase contrast or other I.C. technique and have a minimum of two colour fluorescence.
The new incucyte S3 now has an over-inflated price hike, at least in the U.K., so is no longer looking like something we will be considering.
Any thoughts greatly appreciated.
regards
David Strachan Senior Scientific Officer Beatson Institute for Cancer Research UK
Login Host: 130.209.6.42 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v6OElih5004281 for {MicroscopyListserverArchive6-at-microscopy.com} ; Mon, 24 Jul 2017 09:47:45 -0500 Received: (from mail-at-localhost) by microscopy.com (8.12.11.20060308/8.12.11/Submit) id v6OEliTb004279; Mon, 24 Jul 2017 09:47:44 -0500
Hi everyone, If anyone is using a Cressington 308R for rotary shadowing I would love to hear from you offline to talk about methods. Chris
Christopher J. Gilpin Ph.D. Director, Life Science Microscopy Facility Campus-wide Coordinator for Electron Microscopy Purdue University Whistler Hall of Agriculture Research, Room S052 170 S. University St West Lafayette, IN 47907 765-494-7750 gilpin-at-purdue.edu lsmf-at-purdue.edu reaches everyone in the facility. http://ag.purdue.edu/arp/Microscopy/Pages/default.aspx
==============================Original Headers============================== 5, 32 -- From gilpin-at-purdue.edu Mon Jul 24 09:47:44 2017 5, 32 -- Received: from xppmailspam04.itap.purdue.edu (xppmailspam04.itap.purdue.edu [128.210.5.15]) 5, 32 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v6OEli3W004276 5, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 24 Jul 2017 09:47:44 -0500 5, 32 -- X-IronPort-Anti-Spam-Filtered: true 5, 32 -- X-IronPort-AV: E=Sophos;i="5.40,407,1496116800"; 5, 32 -- d="scan'208";a="13074027" 5, 32 -- Received: from exchange.purdue.edu ([128.210.1.29]) 5, 32 -- by xppmailspam04.itap.purdue.edu with ESMTP/TLS/AES256-SHA; 24 Jul 2017 10:48:05 -0400 5, 32 -- Received: from wppexc03.purdue.lcl (172.30.136.176) by wppexc01.purdue.lcl 5, 32 -- (172.30.136.174) with Microsoft SMTP Server (TLS) id 15.0.1178.4; Mon, 24 Jul 5, 32 -- 2017 10:48:05 -0400 5, 32 -- Received: from wppexc03.purdue.lcl ([fe80::3c86:dcb8:fd99:f174]) by 5, 32 -- wppexc03.purdue.lcl ([fe80::3c86:dcb8:fd99:f174%15]) with mapi id 5, 32 -- 15.00.1178.000; Mon, 24 Jul 2017 10:48:05 -0400 5, 32 -- From: "Gilpin, Christopher J" {gilpin-at-purdue.edu} 5, 32 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 5, 32 -- Subject: rotary shadowing with cressington 308R 5, 32 -- Thread-Topic: rotary shadowing with cressington 308R 5, 32 -- Thread-Index: AdMEi8cv80nYRTvrShSBcE+7XtWarA== 5, 32 -- Date: Mon, 24 Jul 2017 14:48:04 +0000 5, 32 -- Message-ID: {4d083a95fcb8417f9f387e9dc7c0650a-at-wppexc03.purdue.lcl} 5, 32 -- Accept-Language: en-US 5, 32 -- Content-Language: en-US 5, 32 -- X-MS-Has-Attach: 5, 32 -- X-MS-TNEF-Correlator: 5, 32 -- x-ms-exchange-transport-fromentityheader: Hosted 5, 32 -- x-originating-ip: [10.163.23.200] 5, 32 -- Content-Type: text/plain; charset="utf-8" 5, 32 -- MIME-Version: 1.0 5, 32 -- Content-Transfer-Encoding: 8bit 5, 32 -- X-MIME-Autoconverted: from base64 to 8bit by microscopy.com id v6OEli3W004276 ==============================End of - Headers==============================
A technical position is available in the Core Microscopy Facility of The Scripps Research Institute in La Jolla, California. The facility houses electron microscopes (TEM & SEM), as well as confocal (laser scanning & spinning disc), multi-photon, TIRF / STORM and widefield systems. The successful candidate will assist with the operations of the electron microscopy resources (TEM and SEM) of the TSRI Core Microscopy Facility. Duties will include the undertaking electron microscopy projects, assisting users of the facility, providing basic instruction in the use of the electron microscopes, sample preparation (TEM & SEM), minor equipment maintenance (e.g. filament changes) and some administrative work (ordering of supplies and monthly billing). Applicants should have excellent communication and organizational skills, an understanding of laboratory procedures, and the ability to manage a large and varied workload. Minimum qualifications include a bachelor’s degree in Science (with a concentration in Biology), at least 5 years of hands-on experience with electron microscopy, and sample preparation (including sectioning). Computer skills are essential.
To apply, go to the listing on TSRI HR careers website at: https://careers.scripps.edu/postings/11052
or the search the general employments listings (https://careers.scripps.edu/postings/search), using requisition number 04063
-----------------------
Scott Henderson, Ph.D. Professor, Dept. Molecular Medicine Director, TSRI Microscopy Core MB-22 The Scripps Research Institute 10550 North Torrey Pines Rd. La Jolla, CA 92037
From gregw-at-gsk.com Tue Jul 25 02:56:13 2017 Return-Path: {gregw-at-gsk.com} Received: from c03 (host36-228-211-80.serverdedicati.aruba.it [80.211.228.36] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v6P7uCAl017006; Tue, 25 Jul 2017 02:56:13 -0500 Received: from 80.211.228.36 (localhost [IPv6:::1]) by c03 (Postfix) with ESMTPA id 6D60F201B; Mon, 24 Jul 2017 22:33:56 -0400 (EDT) MIME-Version: 1.0 Content-Type: multipart/mixed; boundary="=_fbe3fc2f6eaeb78497b2d114351fe46d"
X-from: stacie-at-ems-secure.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both stacie-at-ems-secure.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Discover the Endless Possibilities... at EMS Microscopy Academy
Message: EMS Microscopy Academy is pleased to announce the Automated and Rapid Specimen Processing Course, which introduces participants to the use of equipment for processing tissue samples for TEM. It is scheduled for Monday and Tuesday, September 11 and 12, in Hatfield, PA.
The preparation of samples for EM requires many steps, with extended wait times in between, requiring an entire day of a technicians time. This process consists of several fixative, wash, dehydration, and resin infiltration steps, which are tedious and prone to temporal variability between runs. With the use of an automated tissue processor or microwave, a technicians time is reduced and better continuity between processing runs is obtained.
To learn more about the class, please copy and paste this webpage into your browser. http://www.emsdiasum.com/microscopy/academy/courses/rapid_processing.aspx
Hope to have you join us! Stacie Kirsch
Login Host: 73.165.127.32 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
*********************************************************************************** Forwarded from "Ask a Microscopist" Please remember that the person asking the question is likely not a member the listserver, and **any reply should go directly to the poster** as well as to the list. Using the "reply" function in your email does *not* send your answer to the person asking the question. Please copy their email address from their question. ***********************************************************************************
Name:Ryan Wilmington
School:College of William & Mary
Grade/Education Level:Undergraduate
US Email:rlwilmington-at-email.wm.edu
What is the relationship between the size of the aperture used before the objective, and the ultimate spot size and spatial resolution of an IR microscope?
If you have a spare SEM filament assembly you are willing to part with please reply to me. I am building an electron flood gun for a high vacuum (thermal) metal evaporator.
From ronaldfaust205-at-gmail.com Wed Jul 26 08:38:31 2017 Return-Path: {ronaldfaust205-at-gmail.com} Received: from gmail.com (mail.dongnamdc.com [222.97.39.100] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v6QDcSJ7014775 for {microscopylistserverarchive6-at-microscopy.com} ; Wed, 26 Jul 2017 08:38:30 -0500 Message-ID: {BCED09EE.71C63C0B-at-gmail.com}
X-from: kuryu-at-rockefeller.edu
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both kuryu-at-rockefeller.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Organization: The Rockefeller University, Electron Microscopy Resource Center
Title-Subject: [Filtered] Assistant Position available
Message: Dear List,
The Rockefeller University, New York, NY, seeks outstanding candidates for Electron Microscopy Resource Center (EMRC). The EMRC provides state of the art electron microscopy support for analysis of a wide variety of biological samples, including virus, bacteria, insects, animal tissue as well as cultured cell and isolated cellular components for structural analyses or immuno-electron microscopy. The EMRC is equipped with two TEMs and one SEM as well as a high pressure freezing and a free substitution unit. Please visit our website for more detail. (http://www.rockefeller.edu/emrc/)
Job Title: Electron Microscopists: Research Support Assistant Employment status: Full time
Department Description: The Electron Microscopy Resource Center (EMRC) is one of the UniversityÂs scientific core facilities, providing expert electron microscopy services and training to researchers for The Rockefeller University and neighboring institutions. The EMRC provides state- of-the-art electron microscopy support for analysis of a wide variety of biological samples, including virus, bacteria, insects, animal tissue as well as cultured cell and isolated cellular components. It conducts structural analyses, immuno-electron microscopy, CLEM, SEM serial block face imaging, montage, and tomography. The EMRC is equipped with three TEMs and two SEM as well as a high pressure freezing and other ambient and cryo EM sample preparation devices.
Job Responsibilities: Will support the Electron Microscopy Resource Center's (EMRC) daily operations, including bench work, sample preparation for transmission and scanning electron microscopy and maintenance of the center. Will be responsible for conventional sample preparation, including processing samples, cutting ultrathin sections, data collection, ordering and receiving supplies, and managing chemical waste compliance and administrative support for office duties.
Job Requirements: Bachelor's degree in science required. Educational emphasis in biology, cell biology, bioengineering or a related field preferred. Must have strong communication skills, ability to work as part of a team, and flexibility to interact with a diverse group of researchers. Must be detail-oriented, focused, highly motivated, and able to work in a team atmosphere for general lab duties, as well as a mentored atmosphere for wide variety research projects. EM hands on experience preferred, but not required. Competency in use of computers is plus, including functional command of Word, Excel,PowerPoint and Photoshop.
Please visit the following site to submit your application: Job: IRC20260
The Rockefeller University is an Equal Opportunity Employer with a policy that forbids discrimination in employment (which includes hiring, terms and conditions, promotion, and termination) on the basis of race, color, religion, sex, age, national origin, citizenship status, marital status, sexual orientation, military status, veteran status, or disability. The Administration has an Affirmative Action Program to increase the employment of women and members of protected classes in all areas of the University's activities.
Login Host: 129.85.110.225 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both rhonda.stroud-at-nrl.navy.mil as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Permanent Position EM Research / Lab management
Message: Permanent Federal Employee Position Available--- The Nanoscale Materials Section at the US Naval Research Laboratory in Washington, DC has an immediate opening for a full-time research scientist / EM lab manager. The Nanoscale Materials Section maintains the DoDs premier electron microscopy facilities for basic research, including a Nion UltraSTEM 200-X for imaging and spectroscopy at scales down to the single atom, a JEOL 2200FS for in situ analysis, and a fully accessorized FEI Helios G3 FIB-SEM. The ideal candidate for this position has aberration-corrected STEM experience, and enjoys pushing the instrumentation to its performance limits. The job duties will be split between collaborative research on nanoscale materials development, and microscope maintenance / trouble-shooting / technique development. A Ph.D. in physics, materials science, chemistry, geology or related field, and US citizenship are required. Interested candidates should email a CV and publication list to rhonda.stroud-at-nrl.navy.mil.
Login Host: 132.250.22.13 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ming_j_zhang-at-amat.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: ming_j_zhang-at-amat.com Name: Ming Zhang
Organization: Applied Materials Company
Title-Subject: [Filtered] FIB Technician Opening
Message: Applied Materials Company has an immediate opening for an FIB technician in Portland, Oregon.
Applied Materials Company is the world's leading semiconductor equipment manufacturing company. It is one of top 500 U.S. Companies and has repeatedly been honored as "Worlds Most Ethical Company" and "100 Best Places to Work in Information Technology".
The successful candidate will cover night shift and mainly focus on TEM sample preparations by hands-on operations of ex-situ and in-situ lift-out systems. He or she must be able to fabricate {100 nm thin lamella with high success rate for TEM observations. A minimum of Bachelor degree or equivalent combination of education and experience in Materials Science, Engineering, Chemistry, or Physics, etc. is required.
US citizen preferred.
Interested individuals should send their resumes to Drew_Goettler-at-amat.com
Login Host: 152.135.235.188 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both pmccurdy-at-colostate.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: pmccurdy-at-colostate.edu Name: Patrick R McCurdy
Organization: Colorado State University
Title-Subject: [Filtered] WetSEM Capsules
Message: I am curious if anyone has had experience with the WetSEM Capsules being marketed by EMS? If anyone with positive or negative experience could email me off-line that would be greatly appreciated.
Thanks, Pat McCurdy
Login Host: 129.82.78.70 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
From buviregi380-at-gmail.com Sun Jul 30 06:00:14 2017 Return-Path: {buviregi380-at-gmail.com} Received: from gmail.com ([221.160.34.117]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v6UB0BSn027143 for {microscopylistserverarchive6-at-microscopy.com} ; Sun, 30 Jul 2017 06:00:13 -0500 Message-ID: {2637AE52.81C76684-at-gmail.com}
X-from: andy.stewart-at-ul.ie
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both andy.stewart-at-ul.ie as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: andy.stewart-at-ul.ie Name: Andy Stewart
Organization: University of Limerick
Title-Subject: [Filtered] Amended advert
Message: Apologies for reposting, the direct weblink in the previous posting does not work. Title: Postdoctoral position in electron microscopy of pharmaceutical crystals. A 3-year postdoctoral position in TEM is available at the University of Limerick, Ireland As part of the H2020 award MagnaPharm (https://www.magnapharm.com) the aim of this research project is to control polymorphism in pharmaceutical crystal growth via magnetic fields, and to solve crystal structure and morphology of these compounds using electron imaging and diffraction methods as well as to build a matrix of machine conditions for data evaluation on organic crystals. Work will be carried out using a double corrected, monochromated Titan Themis, equipped with EDS and EELS, K2-IS and Oneview detectors, as well as in-situ holders (DENS lightning double tilt , DENS ocean, DENS atmosphere, Fischione cryo-transfer holder.) Please apply here: ul.ie Vacancies Job ID: 023368
Closing date is the 11th of August 2017. For further information please contact Ursel.Bangert-at-ul.ie or Andy.Stewart-at-ul.ie
Login Host: 193.1.100.65 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both leandro.lemgrubersoares-at-glasgow.ac.uk as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Problem with sectioning Flat embedded sample
Message: Dear All,
I'm having a specific problem while trying to section a Flat embedded sample (in glass bottom petri dishes) in Epoxy resin. When the section hits the water, the resin expands, and keep expanding until it reaches almost 3-4 times the size of the block face. And the sections are quite sticky as well, making impossible to collect them (they stay glued to the eyelash). I have already processed samples before without a problem, but for the past months it has been the same problem. I don't think its the resin because cell pellets embedded in the same resin can be sectioned normally. Any advise would be great.
Thanks!
Leandro
Login Host: 130.209.127.194 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
X-from: Michael Delannoy {mdelann1-at-jhmi.edu} To: microscopy.listserver-at-gmail.com, delannoy-at-jhmi.edu
Leandro, It sounds like an incomplete infiltration of your resin. Normally for cells on coverslips, we do an overnight in pure epon (after complete a dehydration series), no ethanol:epon parts. The next day we do 3 -2 hr changes of fresh resin (rock, 15 psi vacuum, rock), then cure the resin/coverslip to a pre-filled beem capsules and bake 60C overnight. The following morning we detach the coverslip from the polymerized block by submerging in liquid nitrogen. The coverslips fall off within 5-10 min. Cells are at the beem capsule surface.
Sincerely, Michael Delannoy
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Tuesday, August 01, 2017 11:42 AM To: delannoy-at-jhmi.edu
X-from: leandro.lemgrubersoares-at-glasgow.ac.uk
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both leandro.lemgrubersoares-at-glasgow.ac.uk as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Problem with sectioning Flat embedded sample
Message: Dear All,
I'm having a specific problem while trying to section a Flat embedded sample (in glass bottom petri dishes) in Epoxy resin. When the section hits the water, the resin expands, and keep expanding until it reaches almost 3-4 times the size of the block face. And the sections are quite sticky as well, making impossible to collect them (they stay glued to the eyelash). I have already processed samples before without a problem, but for the past months it has been the same problem. I don't think its the resin because cell pellets embedded in the same resin can be sectioned normally. Any advise would be great.
Thanks!
Leandro
Login Host: 130.209.127.194 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
We are pleased to announce that the Electron Microscopy Core Imaging Facility (EMCIF) at the University of Maryland Baltimore will be offering a Basic Ultramicrotomy mini-course on September 14th and 15th, 2017. This course is designed to teach novice users of ultramicrotome operation at room temperature, trimming and sectioning of biological specimen embedded in epoxy or acrylic resin. It can also serve as a refresher course for current ultramicrotome operators as the course covers the instrumentation, handling and choosing diamond knives and troubleshooting.
The course will include lectures, demonstration and (lots of) hands on practice. Registration will be limited to a maximum of six participants. More information regarding to the course and registration can be found inour website
Ru-ching Hsia Associate Professor and Director rhsia-at-umaryland.edu Electron Microscopy Core Imaging Facility University of Maryland, Baltimore http://www.dental.umaryland.edu/Core-imaging
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both tayl1238-at-vandals.uidaho.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: tayl1238-at-vandals.uidaho.edu Name: Martin Taylor
Organization: University of Idaho
Title-Subject: [Filtered] EBSD
Message: Regarding a metallic sample. I read that the sample should have the Normal direction facing the EBSD detector (at 70 degrees) for pole figures, but does the normal direction need to be facing the detector for Euler, Schmid, Normal, Transverse, and Rolling maps? Login Host: 129.101.212.210 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
From normanatlas54-at-gmail.com Thu Aug 3 04:06:45 2017 Return-Path: {normanatlas54-at-gmail.com} Received: from gmail.com (mail.dongnamdc.com [222.97.39.100] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v7396g70024317 for {microscopylistserverarchive6-at-microscopy.com} ; Thu, 3 Aug 2017 04:06:44 -0500 Message-ID: {9c7801d30bf2$78970270$b1b93524-at-normanatlas54} Reply-To: "Perry Fox" {normanatlas54-at-gmail.com}
Hi Taylor,
The coordinate systems are a matter of convention rather than any particular technical need. A lot of samples that have EBSD performed on them aren't rolled. The important thing when making pole figures and maps is that you can relate the data supplied (and any anisotropy observed) back to the greater context of your sample in some way. If you don't care about how the maps relate to that greater context for whatever reason, then the directions won't really matter to you.
-Jacob
On 2017-08-02 07:36 PM, microscopy.listserver-at-gmail.com wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: tayl1238-at-vandals.uidaho.edu } } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } tayl1238-at-vandals.uidaho.edu as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: tayl1238-at-vandals.uidaho.edu Name: Martin Taylor } } Organization: University of Idaho } } Title-Subject: [Filtered] EBSD } } Message: Regarding a metallic sample. I read that the sample should have the Normal direction facing } the EBSD detector (at 70 degrees) for pole figures, but does the normal direction need to be facing } the detector for Euler, Schmid, Normal, Transverse, and Rolling maps? } Login Host: 129.101.212.210 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 9, 52 -- From microscopy.listserver-at-gmail.com Wed Aug 2 21:15:03 2017 } 9, 52 -- Received: from mail-it0-f51.google.com (mail-it0-f51.google.com [209.85.214.51]) } 9, 52 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v732F3Q7024259 } 9, 52 -- for {microscopy-at-microscopy.com} ; Wed, 2 Aug 2017 21:15:03 -0500 } 9, 52 -- Received: by mail-it0-f51.google.com with SMTP id m34so613767iti.1 } 9, 52 -- for {microscopy-at-microscopy.com} ; Wed, 02 Aug 2017 19:16:07 -0700 (PDT) } 9, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 9, 52 -- d=gmail.com; s=20161025; } 9, 52 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 9, 52 -- :in-reply-to:content-language:content-transfer-encoding; } 9, 52 -- bh=RSt+MZd45SuwCH66n4Z1Gkj13RrpSNKkmvLsoDPsMak=; } 9, 52 -- b=FL7wrw/DyvY76G67O5TZzskMhCMymhysB/xaCuUnCcoK1fCGM6/YGrSRJuv6zM8m5v } 9, 52 -- YdGJZQbNRX0u3Y07nNnURBlIA/h0e/6VrMnwM+4SyvJgz3khSd8ipYYqUdFtx1NK0Cdu } 9, 52 -- GtHW3u0xdy/nylpXZxtO1kkby1010GDGcXyN3g18nDwelUoAmqCblHGct4AtZ4T5cWTg } 9, 52 -- EtVBWnHTAjv48HSh+gBYndQ1LvbGlF7nKUBPnB8N/qE6INpHvr08wKo/Rg4iggvNQiHw } 9, 52 -- kBdQEhPiopCsOr5PbnPOSpGwtBXBEn7SfqPcmAaZBvwrvwaYgnDkFOxjYOvzsY4+BeAn } 9, 52 -- fyLQ== } 9, 52 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 9, 52 -- d=1e100.net; s=20161025; } 9, 52 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 9, 52 -- :user-agent:mime-version:in-reply-to:content-language } 9, 52 -- :content-transfer-encoding; } 9, 52 -- bh=RSt+MZd45SuwCH66n4Z1Gkj13RrpSNKkmvLsoDPsMak=; } 9, 52 -- b=MZtmvRJcvHyjjxxKOmacL67/7tnEfNuzDSE+6lJfXuTV/8E6JzJ8E9QWlb7zdnr4JS } 9, 52 -- 23zSkReffFDAUfzlDlljSYjMsEFyQlPwbaYfB13yt90i6twuMwggXwkVzDoD1bg5LBIS } 9, 52 -- OHSM9F2TOYAhWkLm/O/kd9O1F5iSvfhyzcBwApj55RrFyK/rU7PA9vmGVbTxPiQDlyEt } 9, 52 -- TdjIV3jFTOFHxuDVFWa3Tw16cdiKw0XKBtBtix2neclCNeB6pw4omgleI11c/D4Kh8cE } 9, 52 -- GXe1Q1XlBiLRDpWkToBIt5D2OwTaAPoF4YdqIr5S8/bimy93HHHZuB0xcoM7Jc8lujkt } 9, 52 -- nJEQ== } 9, 52 -- X-Gm-Message-State: AIVw112sZ69JKtYCleECiO3SLFe0LdDqStkPdiGvXQo6YC91R+UodDt4 } 9, 52 -- M6ZmJh2LpuU0k0/gw6U= } 9, 52 -- X-Received: by 10.36.152.198 with SMTP id n189mr141227itd.79.1501726567251; } 9, 52 -- Wed, 02 Aug 2017 19:16:07 -0700 (PDT) } 9, 52 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:c1cb:d7ba:ac75:afba]) } 9, 52 -- by smtp.googlemail.com with ESMTPSA id e16sm2779242itc.10.2017.08.02.19.16.06 } 9, 52 -- for {microscopy-at-microscopy.com} } 9, 52 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 9, 52 -- Wed, 02 Aug 2017 19:16:06 -0700 (PDT) } 9, 52 -- Subject: viaWWW:EBSD Question } 9, 52 -- References: {201708021752.v72HqKsw032250-at-microscopy.com} } 9, 52 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 9, 52 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 9, 52 -- X-Forwarded-Message-Id: {201708021752.v72HqKsw032250-at-microscopy.com} } 9, 52 -- Message-ID: {00379186-0f1d-6b48-f491-4de653c8a145-at-gmail.com} } 9, 52 -- Date: Wed, 2 Aug 2017 21:16:04 -0500 } 9, 52 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) } 9, 52 -- Gecko/20100101 Thunderbird/52.2.1 } 9, 52 -- MIME-Version: 1.0 } 9, 52 -- In-Reply-To: {201708021752.v72HqKsw032250-at-microscopy.com} } 9, 52 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 9, 52 -- Content-Language: en-US } 9, 52 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
-- Jacob Kabel Electron Microscopist Department of Materials Engineering The University of British Columbia 6350 Stores Road, Room 419 Vancouver, British Columbia Canada V6T 1Z4 Phone: (604) 822-5648 Fax: (604) 822-3619 http://emlab.mtrl.ubc.ca/
==============================Original Headers============================== 6, 34 -- From jacob.kabel-at-ubc.ca Thu Aug 3 10:53:11 2017 6, 34 -- Received: from vmaprod5.mail-relay.ubc.ca (vmaprod5.mail-relay.ubc.ca [142.103.117.139]) 6, 34 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v73FrBHE003497 6, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 3 Aug 2017 10:53:11 -0500 6, 34 -- Received: from vmaprod5.mail-relay.ubc.ca (localhost.localdomain [127.0.0.1]) 6, 34 -- by localhost (Email Security Appliance) with SMTP id B61B12D4338_9834729B; 6, 34 -- Thu, 3 Aug 2017 15:54:17 +0000 (GMT) 6, 34 -- Received: from mx2.mail-relay.ubc.ca (lbpglfsc01gstp01-ents01-f5-vrfglue-float.systems.it.ubc.ca [10.45.24.97]) 6, 34 -- (using TLSv1.2 with cipher ECDHE-RSA-AES256-GCM-SHA384 (256/256 bits)) 6, 34 -- (Client CN "mx2.mail-relay.ubc.ca", Issuer "mx2.mail-relay.ubc.ca" (not verified)) 6, 34 -- by vmaprod5.mail-relay.ubc.ca (Sophos Email Appliance) with ESMTPS id 645642D1544_9834729F; 6, 34 -- Thu, 3 Aug 2017 15:54:17 +0000 (GMT) 6, 34 -- Received: from smtp.mail.ubc.ca (s-itsv-hub02p.ead.ubc.ca [137.82.151.71]) 6, 34 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 6, 34 -- (Client CN "rpc.mail.ubc.ca", Issuer "Entrust Certification Authority - L1K" (not verified)) 6, 34 -- by mx2.mail-relay.ubc.ca (Postfix) with ESMTPS id 52EC215F922; 6, 34 -- Thu, 3 Aug 2017 08:54:17 -0700 (PDT) 6, 34 -- Received: from [137.82.16.30] (10.19.170.1) by smtp.mail.ubc.ca 6, 34 -- (137.82.151.71) with Microsoft SMTP Server id 14.3.319.2; Thu, 3 Aug 2017 6, 34 -- 08:54:16 -0700 6, 34 -- Subject: Re: [Microscopy] viaWWW:EBSD Question 6, 34 -- To: {tayl1238-at-vandals.uidaho.edu} , {Microscopy-at-microscopy.com} 6, 34 -- References: {201708030236.v732aSeg012573-at-microscopy.com} 6, 34 -- From: Jacob Kabel {jacob.kabel-at-ubc.ca} 6, 34 -- Message-ID: {b228e688-250c-914b-cf27-402022b87b1c-at-ubc.ca} 6, 34 -- Date: Thu, 3 Aug 2017 08:54:16 -0700 6, 34 -- User-Agent: Mozilla/5.0 (X11; Linux i686; rv:52.0) Gecko/20100101 6, 34 -- Thunderbird/52.2.1 6, 34 -- MIME-Version: 1.0 6, 34 -- In-Reply-To: {201708030236.v732aSeg012573-at-microscopy.com} 6, 34 -- Content-Type: text/plain; charset="utf-8"; format=flowed 6, 34 -- Content-Transfer-Encoding: 7bit 6, 34 -- Content-Language: en-US 6, 34 -- X-Originating-IP: [10.19.170.1] ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both na2661-at-columbia.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: na2661-at-columbia.edu Name: Nava Ariel-Sternberg
Organization: Columbia University
Title-Subject: [Filtered] EM Lab Director
Message: The Columbia Nano Initiative (CNI) at The Fu Foundation School of Engineering & Applied Science of Columbia University in the City of New York invites applications for a Staff Associate or Senior Staff Associate position, serving as an Electron Microscopy Lab Director. Reporting to the Shared Facilities Director, the Electron Microscopy lab director will be responsible for all aspects of the Electron Microscopy Facility, including: implementing safety regulations and procedures, monitoring the performance of equipment; performing routine maintenance, calibration, and repairs and coordinating service calls; assisting in the development and upgrade of instruments including benchmarking and negotiating with vendors; assisting in writing proposals and raising funds for instrument acquisition and upgrade and other microscopy-related projects and equipment; preparation of usage forecasts and assistance with strategic planning; supervising lab assistants. The EM Lab Director will prepare user training documents; perform training of new users; monitor usage and maintain user records; assist in teaching classes, modules and short courses that address the needs of facility users; assist in preparation and teaching of specialized course materials and Electron Microscopy courses. The EM director also will provide training, technical assistance, and advice to users on specimen preparation, operation of instruments and interpretation of data; provide advice and assistance and/or assign an appropriate subordinate to meet requests of users; operate instruments, obtain and interpret data in accordance with specific requests from users; provide tours and demonstrations of the Electron Microscopy Facility; develop a vibrant external users program. Required: Bachelor'ss degree or higher in an engineering or science field. Minimum of four years' hands-on experience in operation and/or maintenance of scanning and transmission electron microscopes. Preference given to candidates with electron microscopy related thesis projects. Application link: https://academicjobs.columbia.edu/applicants/jsp/shared/frameset/Frameset.jsp?time=1501788707343
Login Host: 128.59.87.64 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both miketoalson-at-gmail.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: miketoalson-at-gmail.com Name: Mike Toalson
Organization: NanoImages, LLC
Title-Subject: [Filtered] SEM in Glovebox with ARGON
Message: Kindly asking the community for some guidance on the use of an SEM or any electronic instrument for that matter, in a glovebox having an Argon atmosphere.
After hours of extensive searching, I have not located any experience with 2 reported concerns:
1) That there can be issues with arcing of circuit boards or exposed electrical components.
2) That motors are subject to overheating - probably only for open motors that are cooled by the air, but how would a sealed Turbo Molecular Pump fare?
3) Argon's breakdown voltage is 20% of that of Air. What effect would this have inside an electronic device like a instrument or SEM?
Thank you so much for any advice!!
Login Host: 47.208.204.209 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both miketoalson-at-gmail.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: miketoalson-at-gmail.com Name: Mike Toalson
Organization: NanoImages, LLC
Title-Subject: [Filtered] Question to Users of Tungsten Filament SEM
Message: Please help me formulate a stance on a debate about the effects of an SEM's Tungsten filament breaking when they fail or "burn out".
One vendor is raising the alarm that if a user waits until a tungsten filament breaks or fails prior to replacing it, that they run the risk of experiencing a much bigger cleanup issue and even having "shards" from the filament passing over to the Turbo Pump turbines and causing damage.
I've seen plenty of burned out Tungsten filaments but never experienced these issues. Of course, the Wehnelt or Anode can often need a good cleaning which is why most labs have some Pikal or Wenol paste handy.
Other than the interruption to replace a filament, has anybody with a Thermonic Tungsten Filament SEM ever have a major problem from the filament burning out?
Any thoughts on this matter are much appreciated!!
Mike Toalson NanoImages, LLC
Login Host: 47.208.204.209 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both miketoalson-at-gmail.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: miketoalson-at-gmail.com Name: Mike Toalson
Organization: NanoImages, LLC
Title-Subject: [Filtered] SEM in Glovebox with ARGON
Message: Kindly asking the community for some guidance on the use of an SEM or any electronic instrument for that matter, in a glovebox having an Argon atmosphere.
After hours of extensive searching, I have not located any experience with 2 reported concerns:
1) That there can be issues with arcing of circuit boards or exposed electrical components.
2) That motors are subject to overheating - probably only for open motors that are cooled by the air, but how would a sealed Turbo Molecular Pump fare?
3) Argon's breakdown voltage is 20% of that of Air. What effect would this have inside an electronic device like a instrument or SEM?
Thank you so much for any advice!!
Login Host: 47.208.204.209 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
1) heat transfer - Argon has about 67% of thermal conductivity of air at normal pressure / T making overheating of electrical components quite possible. Watch your thermocouple and pirani vacuum gauges for starts. Forced cooling (as in motors with fans) won't help much because specific heat of Ar is about half of that of air.
2) breakdown voltage of Argon is 0.2 of air. Circuits with voltages above low ones (5 / 12 / 24 V) will be at risk.
more points?
Vitaly Feingold SIA 2773 Heath Lane Duluth GA 30096 Ph. 770-232-7785 Fax 770-232-1791 www.sia-cam.com vitaly-at-sia-cam.com
On 8/3/2017 9:26 PM, microscopy.listserver-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: miketoalson-at-gmail.com } } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } miketoalson-at-gmail.com as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: miketoalson-at-gmail.com Name: Mike Toalson } } Organization: NanoImages, LLC } } Title-Subject: [Filtered] SEM in Glovebox with ARGON } } Message: Kindly asking the community for some guidance on the use of an SEM or any electronic } instrument for that matter, in a glovebox having an Argon atmosphere. } } After hours of extensive searching, I have not located any experience with 2 reported concerns: } } 1) That there can be issues with arcing of circuit boards or exposed electrical components. } } 2) That motors are subject to overheating - probably only for open motors that are cooled by the } air, but how would a sealed Turbo Molecular Pump fare? } } 3) Argon's breakdown voltage is 20% of that of Air. What effect would this have inside an electronic } device like a instrument or SEM? } } Thank you so much for any advice!! } } Login Host: 47.208.204.209 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 15, 52 -- From microscopy.listserver-at-gmail.com Thu Aug 3 20:24:08 2017 } 15, 52 -- Received: from mail-io0-f178.google.com (mail-io0-f178.google.com [209.85.223.178]) } 15, 52 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v741O72m002585 } 15, 52 -- for {microscopy-at-microscopy.com} ; Thu, 3 Aug 2017 20:24:08 -0500 } 15, 52 -- Received: by mail-io0-f178.google.com with SMTP id m88so1143435iod.2 } 15, 52 -- for {microscopy-at-microscopy.com} ; Thu, 03 Aug 2017 18:25:16 -0700 (PDT) } 15, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 15, 52 -- d=gmail.com; s=20161025; } 15, 52 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 15, 52 -- :in-reply-to:content-language:content-transfer-encoding; } 15, 52 -- bh=TFprenRbZnADAynI5ecA+hWLUbBFtyA+tEIsNErRRJY=; } 15, 52 -- b=M0vymHds3X9p5BnwEI3Mkt1Sj8nIlWNUIhQlLRq8KkWx1DBzEV4kgGOzabafVofOR3 } 15, 52 -- NDlL3O6UjeKqAgKe5DmNRzhx4PEaNcrr4kWwyNsBq80wUZfchEpRAxDFfqXTlTBi7MZ0 } 15, 52 -- LkbQwW0hW/OtYUV1ST/N+t22eZ7ur7Xpjpa2nhbvH2g2wrDkaVmSBwsI4pUcKZR4TceR } 15, 52 -- KzuKUn/bsVmjVVqlekNU/8Q5Khr07Ew2HDLhsPalT8VxZzvoltJsf3He18mQE6vMw0AI } 15, 52 -- QAs+2HJL7kUj7ktRLVcOCrteTPh6kffsw3aABbbMHBTX6RspXLLnPE8hT8eaRXOWBbP0 } 15, 52 -- MUAQ== } 15, 52 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 15, 52 -- d=1e100.net; s=20161025; } 15, 52 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 15, 52 -- :user-agent:mime-version:in-reply-to:content-language } 15, 52 -- :content-transfer-encoding; } 15, 52 -- bh=TFprenRbZnADAynI5ecA+hWLUbBFtyA+tEIsNErRRJY=; } 15, 52 -- b=epDaDhc/fbvZoa4d36d+PDOtn3p0PKqMsr4fkIBctAmwlWG3OXu619jupgwKlXj0bQ } 15, 52 -- ul423STHtxUBVotGyDTvuazFgUhGSX+qRmpMfURoflBb7RCcVE4b3a4KwRmZpgiJc8tN } 15, 52 -- B8+1jUEJgCQBtH2tu9z+zCRotk2cNJJAxkRaFOg6Z27sWvOXVjt8Mhgj9La0wuyd45Ay } 15, 52 -- rDOfOsd4vYcRocR/ZBok/nKlk1WHWcnN9jDLbYH0TL2dHcMb4MgDmhWRQfcRIOTeA0GI } 15, 52 -- 5Tal7qqAOjQ1zPOuVk2UAXsLYt2wZ6fsVAXNyXBdUkdJ6piXLc+a3pTQ3kUBaCubWgNs } 15, 52 -- f0Vg== } 15, 52 -- X-Gm-Message-State: AIVw110fVC4tsuI8cidkKXIcl2WfDgiDacXP/rD/mkh08YwR2fVS9o7K } 15, 52 -- zcmgea68rMqTmkw7xAc= } 15, 52 -- X-Received: by 10.107.40.212 with SMTP id o203mr798026ioo.181.1501809915494; } 15, 52 -- Thu, 03 Aug 2017 18:25:15 -0700 (PDT) } 15, 52 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:9148:ca52:ad23:948c]) } 15, 52 -- by smtp.googlemail.com with ESMTPSA id b14sm153878itb.18.2017.08.03.18.25.14 } 15, 52 -- for {microscopy-at-microscopy.com} } 15, 52 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 15, 52 -- Thu, 03 Aug 2017 18:25:15 -0700 (PDT) } 15, 52 -- Subject: viaWWW:SEM in Glovebox with ARGON } 15, 52 -- References: {201708040059.v740xXsf031443-at-microscopy.com} } 15, 52 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 15, 52 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 15, 52 -- X-Forwarded-Message-Id: {201708040059.v740xXsf031443-at-microscopy.com} } 15, 52 -- Message-ID: {8e0000d6-ccfc-8c6e-df7d-1061fc4a8335-at-gmail.com} } 15, 52 -- Date: Thu, 3 Aug 2017 20:25:14 -0500 } 15, 52 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) } 15, 52 -- Gecko/20100101 Thunderbird/52.2.1 } 15, 52 -- MIME-Version: 1.0 } 15, 52 -- In-Reply-To: {201708040059.v740xXsf031443-at-microscopy.com} } 15, 52 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 15, 52 -- Content-Language: en-US } 15, 52 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
==============================Original Headers============================== 7, 33 -- From vitalylazar-at-att.net Thu Aug 3 21:05:17 2017 7, 33 -- Received: from nm2-vm10.access.bullet.mail.bf1.yahoo.com (nm2-vm10.access.bullet.mail.bf1.yahoo.com [216.109.114.83]) 7, 33 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v7425HZN002733 7, 33 -- for {Microscopy-at-microscopy.com} ; Thu, 3 Aug 2017 21:05:17 -0500 7, 33 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=att.net; s=s1024; t=1501812385; bh=ciXrwMzdDCMvPGgGIIy6WDCuW5sa8isOu0cPI/7g840=; h=Date:From:To:Subject:References:In-Reply-To:From:Subject; b=IJE+tpicIunxKEnt0C/H5XC1vBGeJAehI1zQO1HvQhhyVP3GSUBK5ahC6NnYIRRO+HgEP+D07TWR5brpvhI/WVoddfnauR1VzemF1KHTvBZzhXFcOHoh/LCOlmbaP8S3RFEToPNv0UxOqhFjqimg4/zuWRsad1nLKeTtKc2uiDM= 7, 33 -- Received: from [66.196.81.161] by nm2.access.bullet.mail.bf1.yahoo.com with NNFMP; 04 Aug 2017 02:06:25 -0000 7, 33 -- Received: from [98.138.226.244] by tm7.access.bullet.mail.bf1.yahoo.com with NNFMP; 04 Aug 2017 02:06:25 -0000 7, 33 -- Received: from [127.0.0.1] by smtp115.sbc.mail.ne1.yahoo.com with NNFMP; 04 Aug 2017 02:06:25 -0000 7, 33 -- X-Yahoo-Newman-Id: 124932.16482.bm-at-smtp115.sbc.mail.ne1.yahoo.com 7, 33 -- X-Yahoo-Newman-Property: ymail-3 7, 33 -- X-YMail-OSG: J_QLaTAVM1mcz2QMyNVVEGBXWJGki8KR8TFGrkjREXM0fUA 7, 33 -- odS2VO15aHsNmUVXVuOdBn1GruFSsynUIQe5Nyi7Av8aq.HSP5mv66osT0vg 7, 33 -- hCxdSUoKZ6EZVE20i5vsfjTwImFcq17TdNg6w3.T3e_kMlfJfes94dHy46L7 7, 33 -- gOOl2a0eicwYrYqWuRrsQJbe.K7Mno_SV3AqXbHxDJmhJMUc91m2.qeIpv4A 7, 33 -- SSvu.1EgcCJ31uIX7B8z0CfWoNWKYHBi3_wUgp4ATircBr0j5RZ.HV_7yDI1 7, 33 -- p2NTEqIX_GXHHPCi_ERFPC27kU9xP8cb2xITq_RVq2mGqRxbijiPydviRxRy 7, 33 -- TQ7g_sP_cyTJ_KVVp7TnnfMf73qcmo.OFUTPU1IZGIWhRa9DLh.DgToJhfA. 7, 33 -- yW8koczw28T9J46FYjNnLYurNcnPoa2_7GwLObVjKZC8YJdxrsiLTzaSpmiz 7, 33 -- WOfZHIxWWyLrYditB4lIiHaH3qI4BFKOJwjgAFxf7EmFIkGB5QGcDW.tPEOn 7, 33 -- n4zRubbzWLcnBY.9UW4qblM860aVWB49v_GrCuyMdHsM- 7, 33 -- X-Yahoo-SMTP: gj.qIISswBA.S8Qm9l.Bw9ouZ8hEcT1EKbhSAYjFDs8- 7, 33 -- Message-ID: {5983D6A1.7040306-at-att.net} 7, 33 -- Date: Thu, 03 Aug 2017 22:06:25 -0400 7, 33 -- From: Vitaly Feingold {vitalylazar-at-att.net} 7, 33 -- User-Agent: Mozilla/5.0 (Windows NT 6.3; WOW64; rv:31.0) Gecko/20100101 Thunderbird/31.4.0 7, 33 -- MIME-Version: 1.0 7, 33 -- To: microscopy.listserver-at-gmail.com, 7, 33 -- Microscopy Listserver {Microscopy-at-microscopy.com} 7, 33 -- Subject: Re: [Microscopy] viaWWW:SEM in Glovebox with ARGON 7, 33 -- References: {201708040126.v741Q8dX006208-at-microscopy.com} 7, 33 -- In-Reply-To: {201708040126.v741Q8dX006208-at-microscopy.com} 7, 33 -- Content-Type: text/plain; charset=windows-1252; format=flowed 7, 33 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Tungsten filament won't cause any problems beyond periodic routine maintenance (cleaning) of gun components unless electrical malfunction turns it into a photo-flash all the time - which is extremely unlikely. Wouldn't worry about that.
Vitaly Feingold SIA 2773 Heath Lane Duluth GA 30096 Ph. 770-232-7785 Fax 770-232-1791 www.sia-cam.com vitaly-at-sia-cam.com
On 8/3/2017 9:24 PM, microscopy.listserver-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: miketoalson-at-gmail.com } } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } miketoalson-at-gmail.com as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: miketoalson-at-gmail.com Name: Mike Toalson } } Organization: NanoImages, LLC } } Title-Subject: [Filtered] Question to Users of Tungsten Filament SEM } } Message: Please help me formulate a stance on a debate about the effects of an SEM's Tungsten } filament breaking when they fail or "burn out". } } One vendor is raising the alarm that if a user waits until a tungsten filament breaks or fails prior } to replacing it, that they run the risk of experiencing a much bigger cleanup issue and even having } "shards" from the filament passing over to the Turbo Pump turbines and causing damage. } } I've seen plenty of burned out Tungsten filaments but never experienced these issues. Of course, } the Wehnelt or Anode can often need a good cleaning which is why most labs have some Pikal or Wenol } paste handy. } } Other than the interruption to replace a filament, has anybody with a Thermonic Tungsten Filament } SEM ever have a major problem from the filament burning out? } } Any thoughts on this matter are much appreciated!! } } Mike Toalson } NanoImages, LLC } } Login Host: 47.208.204.209 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 15, 52 -- From microscopy.listserver-at-gmail.com Thu Aug 3 20:22:33 2017 } 15, 52 -- Received: from mail-io0-f180.google.com (mail-io0-f180.google.com [209.85.223.180]) } 15, 52 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v741MWUr000546 } 15, 52 -- for {microscopy-at-microscopy.com} ; Thu, 3 Aug 2017 20:22:32 -0500 } 15, 52 -- Received: by mail-io0-f180.google.com with SMTP id g71so1066556ioe.5 } 15, 52 -- for {microscopy-at-microscopy.com} ; Thu, 03 Aug 2017 18:23:40 -0700 (PDT) } 15, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 15, 52 -- d=gmail.com; s=20161025; } 15, 52 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 15, 52 -- :in-reply-to:content-language:content-transfer-encoding; } 15, 52 -- bh=v4FPnX2sFhva5ns+3Hr4n+H9Ljr9ZfgXSe6JVTCR2ek=; } 15, 52 -- b=ivDQULcUle3PAXgxH7h47j3LN+ylbBwdydF8FGvvCgIQ5AjQ/Vy4rK0ghOPEYCDAIY } 15, 52 -- Th8zv2McvOjro52JRIIo/5GSX/UehsAgQbgrk030qo0mFEKm7SNdJMArNUSVOl5e3c2B } 15, 52 -- iwoAKX3lbdwcO9At2TEcsZgwnAhCXs8t7p2Soo0sczWcBSUeY1TFLK++lEqvt+F3WxCB } 15, 52 -- VcVBMKo0cIXm5kJSJZC0PvUNBzTSoryubE42ff04awNkPln7tHngYeIzuF4oQYLfFR9W } 15, 52 -- Z7lF3nV059iNN64OpbVrtJd+AqR1AT5Ge2NERe621JQXKGP4VpM7kkxA31yZTYd75ZkM } 15, 52 -- C/tw== } 15, 52 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 15, 52 -- d=1e100.net; s=20161025; } 15, 52 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 15, 52 -- :user-agent:mime-version:in-reply-to:content-language } 15, 52 -- :content-transfer-encoding; } 15, 52 -- bh=v4FPnX2sFhva5ns+3Hr4n+H9Ljr9ZfgXSe6JVTCR2ek=; } 15, 52 -- b=qThQeH2Fqv7cgD/a23MwN0xX2wMnSrsWgAgRVYPyj20dhY19IkWLM07PKzclXf8O3C } 15, 52 -- 3uDGYqZrNhy0Me4YiiIUdpLEekXMPJrp0s8y3tUe9yT/T2/sy9CgSQ3NKvLuy7QSkUD1 } 15, 52 -- d9baf5GRgnZxtwZkJHpuWgJCnclZwlBiYeb3Gb8RVj/Xgqy0SS0JTmD+pQCUkM4PZf7J } 15, 52 -- YhZZf1SVp1dK3FpmTJU1Cjf/fFFGejmGdVMrSofwTQydX60g+50pPjeVWb/+XX6mAcV9 } 15, 52 -- 3nUPQv1huR+CLnEukJUgBihgwH/iV4LirMwM4nswnmwHJdRX65tqeI7i97PJHWC3Oj8E } 15, 52 -- ntaw== } 15, 52 -- X-Gm-Message-State: AHYfb5jKDw2zBzebk0GsPpsTV5h97i2+GrauSDK6lqPY5PWKX62bst1M } 15, 52 -- QFJeo+MIqQwgtSwzk9w= } 15, 52 -- X-Received: by 10.107.170.39 with SMTP id t39mr839237ioe.9.1501809819771; } 15, 52 -- Thu, 03 Aug 2017 18:23:39 -0700 (PDT) } 15, 52 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:28c9:71b1:9c5f:2932]) } 15, 52 -- by smtp.googlemail.com with ESMTPSA id i190sm1319668itb.3.2017.08.03.18.23.38 } 15, 52 -- for {microscopy-at-microscopy.com} } 15, 52 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 15, 52 -- Thu, 03 Aug 2017 18:23:39 -0700 (PDT) } 15, 52 -- Subject: viaWWW:Question to Users of Tungsten Filament SEM } 15, 52 -- References: {201708040114.v741EPTq031824-at-microscopy.com} } 15, 52 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 15, 52 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 15, 52 -- X-Forwarded-Message-Id: {201708040114.v741EPTq031824-at-microscopy.com} } 15, 52 -- Message-ID: {f76964ae-7e9a-2d0b-2997-eb40eaeb5207-at-gmail.com} } 15, 52 -- Date: Thu, 3 Aug 2017 20:23:38 -0500 } 15, 52 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) } 15, 52 -- Gecko/20100101 Thunderbird/52.2.1 } 15, 52 -- MIME-Version: 1.0 } 15, 52 -- In-Reply-To: {201708040114.v741EPTq031824-at-microscopy.com} } 15, 52 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 15, 52 -- Content-Language: en-US } 15, 52 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
==============================Original Headers============================== 4, 33 -- From vitalylazar-at-att.net Thu Aug 3 21:18:49 2017 4, 33 -- Received: from nm23-vm9.access.bullet.mail.bf1.yahoo.com (nm23-vm9.access.bullet.mail.bf1.yahoo.com [216.109.115.168]) 4, 33 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v742ImEV014712 4, 33 -- for {Microscopy-at-microscopy.com} ; Thu, 3 Aug 2017 21:18:48 -0500 4, 33 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=att.net; s=s1024; t=1501813197; bh=NZziRSn9Ne/JkQ+095XTbmqDR5ghf7r7ajsbQmP3uiM=; h=Date:From:To:Subject:References:In-Reply-To:From:Subject; b=BuLs5ruuXWI2HrojeXVquzM3Cx/hiI4CfV9vABwP1WUz0mvj2jqkqwKKVeDMozcxnzPiAEGUW0LYXJDAlF8zb8UpZGBKp8pfG32KOf7+ZY9b+wY8UpIiRfbwNpDq5u7I8C8+aqiw3aaNUmkKmjF6o8oAzER5lL7qZbMHm0is9RE= 4, 33 -- Received: from [66.196.81.164] by nm23.access.bullet.mail.bf1.yahoo.com with NNFMP; 04 Aug 2017 02:19:57 -0000 4, 33 -- Received: from [98.138.226.244] by tm10.access.bullet.mail.bf1.yahoo.com with NNFMP; 04 Aug 2017 02:19:57 -0000 4, 33 -- Received: from [127.0.0.1] by smtp115.sbc.mail.ne1.yahoo.com with NNFMP; 04 Aug 2017 02:19:56 -0000 4, 33 -- X-Yahoo-Newman-Id: 965537.16480.bm-at-smtp115.sbc.mail.ne1.yahoo.com 4, 33 -- X-Yahoo-Newman-Property: ymail-3 4, 33 -- X-YMail-OSG: p_sf3qwVM1l4.ZoKVwUZjedFTD4EA2XBPpIxZ5Gk9dgUdR_ 4, 33 -- 11ilDl8Yni93Yf6X2CreFiSY1zYOT9cxDUPWR4pLlvwpI2Pel8FypYn25p65 4, 33 -- YJzd4N5N0HOtES9XraKxUkl.j1vwM8Vln0_KtqMdy7H.cQ6LOG0en19tIelh 4, 33 -- LSUwBJkCPUJo1buJVdDRwZYzTIWD2qp.k6kFO87N1zNbQl5P9WUA5Nx7roUs 4, 33 -- 2GETGSDKEKfhUA9rr.dz4XAfLWzT8F_o66B_QMOmGieWy1WbQeA5o095DCN3 4, 33 -- xxCPEUktKMuDZnxKeOOTr.neScjCrioR7IWyPzjAA_qnbFBxmHfpae6q6XvJ 4, 33 -- C.yp7D0QhdwdMZrNRWhrItwuDNr4VY..HQ2nirwVXviYJSgfw4KHNlJnTVLI 4, 33 -- RyqBslq5LRVtJOrZhR7PJC3eltLYQueS1djEl5jnl0U05754OqVMsQBDR_7h 4, 33 -- EW38CkExqFggE0IanEw8KxkTLJ5J_Q0A1pH5xN_Li.0xfE0KLOEDyDdNMJ_Z 4, 33 -- 1y8IWd58ulrqCYs.nW3UjvXT6O.RiJpOR2tme7N8AYDs- 4, 33 -- X-Yahoo-SMTP: gj.qIISswBA.S8Qm9l.Bw9ouZ8hEcT1EKbhSAYjFDs8- 4, 33 -- Message-ID: {5983D9CD.7040101-at-att.net} 4, 33 -- Date: Thu, 03 Aug 2017 22:19:57 -0400 4, 33 -- From: Vitaly Feingold {vitalylazar-at-att.net} 4, 33 -- User-Agent: Mozilla/5.0 (Windows NT 6.3; WOW64; rv:31.0) Gecko/20100101 Thunderbird/31.4.0 4, 33 -- MIME-Version: 1.0 4, 33 -- To: microscopy.listserver-at-gmail.com, 4, 33 -- Microscopy Listserver {Microscopy-at-microscopy.com} 4, 33 -- Subject: Re: [Microscopy] viaWWW:Question to Users of Tungsten Filament SEM 4, 33 -- References: {201708040124.v741OkX2003567-at-microscopy.com} 4, 33 -- In-Reply-To: {201708040124.v741OkX2003567-at-microscopy.com} 4, 33 -- Content-Type: text/plain; charset=windows-1252; format=flowed 4, 33 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Would this vendor by any chance be a vendor of filaments that he wants to sell you? This sounds like a scam. Tungsten evaporates continuously from the filament during its operation. If it is overheated, there can be some melting, but the liquid coalesces into little balls on the ends of the ‘gap’ in the filament. There is nothing that would migrate from the gun to the turbo pump that would cause turbo failure. Pieces of specimen falling off your mounts are the bigger concern.
John Mardinly
} } Remember this posting is most likely not from a Subscriber, so when replying please copy both } miketoalson-at-gmail.com as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: miketoalson-at-gmail.com Name: Mike Toalson } } Organization: NanoImages, LLC } } Title-Subject: [Filtered] Question to Users of Tungsten Filament SEM } } Message: Please help me formulate a stance on a debate about the effects of an SEM's Tungsten } filament breaking when they fail or "burn out". } } One vendor is raising the alarm that if a user waits until a tungsten filament breaks or fails prior } to replacing it, that they run the risk of experiencing a much bigger cleanup issue and even having } "shards" from the filament passing over to the Turbo Pump turbines and causing damage. } } I've seen plenty of burned out Tungsten filaments but never experienced these issues. Of course, } the Wehnelt or Anode can often need a good cleaning which is why most labs have some Pikal or Wenol } paste handy. } } Other than the interruption to replace a filament, has anybody with a Thermonic Tungsten Filament } SEM ever have a major problem from the filament burning out? } } Any thoughts on this matter are much appreciated!! } } Mike Toalson } NanoImages, LLC } }
==============================Original Headers============================== 6, 92 -- From John.Mardinly-at-asu.edu Thu Aug 3 23:08:37 2017 6, 92 -- Received: from bcnete03.asu.edu (bcnete03.asu.edu [13.58.183.106]) 6, 92 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v7448bXt017344 6, 92 -- for {Microscopy-at-Microscopy.com} ; Thu, 3 Aug 2017 23:08:37 -0500 6, 92 -- X-ASG-Debug-ID: 1501819784-0df5cc77ef67d00001-FOsErg 6, 92 -- Received: from mx0b-001d2c01.pphosted.com ([10.120.91.91]) by bcnete03.asu.edu with ESMTP id qYy61Y0GPd4mNla2 (version=TLSv1.2 cipher=ECDHE-RSA-AES256-GCM-SHA384 bits=256 verify=NO); Thu, 03 Aug 2017 21:09:44 -0700 (MST) 6, 92 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu 6, 92 -- X-Barracuda-RBL-Trusted-Forwarder: 10.120.91.91 6, 92 -- Received: from pps.filterd (m0116090.ppops.net [127.0.0.1]) 6, 92 -- by mx0b-001d2c01.pphosted.com (8.16.0.21/8.16.0.21) with SMTP id v7448Gde032700; 6, 92 -- Thu, 3 Aug 2017 21:09:44 -0700 6, 92 -- Received: from nam02-sn1-obe.outbound.protection.outlook.com (mail-sn1nam02lp0022.outbound.protection.outlook.com [216.32.180.22]) 6, 92 -- by mx0b-001d2c01.pphosted.com with ESMTP id 2c4evf85xv-1 6, 92 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES256-SHA384 bits=256 verify=NOT); 6, 92 -- Thu, 03 Aug 2017 21:09:44 -0700 6, 92 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 92 -- d=arizonastateu.onmicrosoft.com; s=selector1-asu-edu; 6, 92 -- h=From:Date:Subject:Message-ID:Content-Type:MIME-Version; 6, 92 -- bh=/ZTU5H233MQVT1Dss5Td+AF3jVIvnCmthjydeY91DIE=; 6, 92 -- b=EHzHclN4lJBzmo7h20EGniWIF56hY3faRMkvxEqumwSZS1WnA14GNW0biaQoP3rXwL6YCd9JYzWhJSVBqybJ4EN2g0BAMho/3ws34eUWk+tBSuGL5ggXvIMyJfK5ZC61wnJuu2aXFxhZgS2OCWw87GlkzMTqjNKrnAdvWTPJpFQ= 6, 92 -- X-Barracuda-RBL-IP: 216.32.180.22 6, 92 -- X-Barracuda-Effective-Source-IP: mail-sn1nam02lp0022.outbound.protection.outlook.com[216.32.180.22] 6, 92 -- X-Barracuda-Apparent-Source-IP: 216.32.180.22 6, 92 -- Received: from CY4PR06MB2904.namprd06.prod.outlook.com (10.175.118.144) by 6, 92 -- CY4PR06MB2902.namprd06.prod.outlook.com (10.175.118.142) with Microsoft SMTP 6, 92 -- Server (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_256_CBC_SHA384_P256) id 6, 92 -- 15.1.1304.22; Fri, 4 Aug 2017 04:09:43 +0000 6, 92 -- Received: from CY4PR06MB2904.namprd06.prod.outlook.com ([10.175.118.144]) by 6, 92 -- CY4PR06MB2904.namprd06.prod.outlook.com ([10.175.118.144]) with mapi id 6, 92 -- 15.01.1304.025; Fri, 4 Aug 2017 04:09:42 +0000 6, 92 -- From: John Mardinly {John.Mardinly-at-asu.edu} 6, 92 -- To: "miketoalson-at-gmail.com" {miketoalson-at-gmail.com} , 6, 92 -- MSA 6, 92 -- {Microscopy-at-Microscopy.com} 6, 92 -- Subject: Re: [Microscopy] viaWWW:Question to Users of Tungsten Filament SEM 6, 92 -- Thread-Topic: [Microscopy] viaWWW:Question to Users of Tungsten Filament SEM 6, 92 -- X-ASG-Orig-Subj: Re: [Microscopy] viaWWW:Question to Users of Tungsten Filament SEM 6, 92 -- Thread-Index: AQHTDML6pkfjUZJ4nkywDcB9O7zR0KJzlXeA 6, 92 -- Date: Fri, 4 Aug 2017 04:09:42 +0000 6, 92 -- Message-ID: {6B6ED2D9-1887-4CF3-AEE4-98A45A66D176-at-asu.edu} 6, 92 -- References: {201708040141.v741fUR8005249-at-microscopy.com} 6, 92 -- In-Reply-To: {201708040141.v741fUR8005249-at-microscopy.com} 6, 92 -- Accept-Language: en-US 6, 92 -- Content-Language: en-US 6, 92 -- X-MS-Has-Attach: 6, 92 -- X-MS-TNEF-Correlator: 6, 92 -- x-originating-ip: [2600:8800:1680:557:f156:3cdb:2aee:fb6d] 6, 92 -- x-ms-publictraffictype: Email 6, 92 -- x-microsoft-exchange-diagnostics: 1;CY4PR06MB2902;7: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 6, 92 -- x-ms-office365-filtering-correlation-id: 253e2689-5944-401b-0274-08d4daeea27a 6, 92 -- x-microsoft-antispam: UriScan:;BCL:0;PCL:0;RULEID:(300000500095)(300135000095)(300000501095)(300135300095)(22001)(300000502095)(300135100095)(2017030254152)(300000503095)(300135400095)(2017052603031)(201703131423075)(201703031133081)(201702281549075)(300000504095)(300135200095)(300000505095)(300135600095)(300000506095)(300135500095);SRVR:CY4PR06MB2902; 6, 92 -- x-ms-traffictypediagnostic: CY4PR06MB2902: 6, 92 -- x-exchange-antispam-report-test: UriScan:(788757137089); 6, 92 -- x-microsoft-antispam-prvs: {CY4PR06MB2902C4C2CBFE8ED13E2785F5E2B60-at-CY4PR06MB2902.namprd06.prod.outlook.com} 6, 92 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:(100000700101)(100105000095)(100000701101)(100105300095)(100000702101)(100105100095)(6040450)(601004)(2401047)(5005006)(8121501046)(93006095)(93001095)(10201501046)(100000703101)(100105400095)(3002001)(6041248)(20161123555025)(20161123558100)(201703131423075)(201702281529075)(201702281528075)(201703061421075)(201703061406153)(20161123564025)(20161123560025)(20161123562025)(6072148)(100000704101)(100105200095)(100000705101)(100105500095);SRVR:CY4PR06MB2902;BCL:0;PCL:0;RULEID:(100000800101)(100110000095)(100000801101)(100110300095)(100000802101)(100110100095)(100000803101)(100110400095)(100000804101)(100110200095)(100000805101)(100110500095);SRVR:CY4PR06MB2902; 6, 92 -- x-forefront-prvs: 0389EDA07F 6, 92 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10009020)(6009001)(39450400003)(39850400002)(39840400002)(39400400002)(39410400002)(39860400002)(189002)(53474002)(199003)(478694002)(6246003)(2906002)(86362001)(25786009)(83716003)(39060400002)(6436002)(97736004)(101416001)(189998001)(75432002)(102836003)(478600001)(38730400002)(3280700002)(53936002)(3660700001)(105586002)(99286003)(106356001)(6116002)(81166006)(2900100001)(6512007)(82746002)(7736002)(305945005)(2950100002)(14454004)(2501003)(88552002)(72206003)(50986999)(8676002)(229853002)(54356999)(77096006)(8936002)(36756003)(5660300001)(81156014)(33656002)(6506006)(6486002)(68736007)(325944009)(76176999);DIR:OUT;SFP:1101;SCL:1;SRVR:CY4PR06MB2902;H:CY4PR06MB2904.namprd06.prod.outlook.com;FPR:;SPF:None;PTR:InfoNoRecords;MX:1;A:1;LANG:en; 6, 92 -- received-spf: None (protection.outlook.com: asu.edu does not designate 6, 92 -- permitted sender hosts) 6, 92 -- spamdiagnosticoutput: 1:99 6, 92 -- spamdiagnosticmetadata: NSPM 6, 92 -- Content-Type: text/plain; charset="utf-8" 6, 92 -- Content-ID: {313AA1F80B194A478F0C1FDBD5FC01DE-at-namprd06.prod.outlook.com} 6, 92 -- MIME-Version: 1.0 6, 92 -- X-OriginatorOrg: asu.edu 6, 92 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 04 Aug 2017 04:09:42.7496 6, 92 -- (UTC) 6, 92 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted 6, 92 -- X-MS-Exchange-CrossTenant-id: 41f88ecb-ca63-404d-97dd-ab0a169fd138 6, 92 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: CY4PR06MB2902 6, 92 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:,, definitions=2017-08-04_01:,, 6, 92 -- signatures=0 6, 92 -- X-Proofpoint-Spam-Details: rule=outbound_notspam policy=outbound score=0 priorityscore=1501 6, 92 -- malwarescore=0 suspectscore=0 phishscore=0 bulkscore=0 spamscore=0 6, 92 -- clxscore=1015 lowpriorityscore=0 impostorscore=0 adultscore=0 6, 92 -- classifier=spam adjust=0 reason=mlx scancount=1 engine=8.0.1-1706020000 6, 92 -- definitions=main-1708040061 6, 92 -- X-Barracuda-Connect: UNKNOWN[10.120.91.91] 6, 92 -- X-Barracuda-Start-Time: 1501819784 6, 92 -- X-Barracuda-Encrypted: ECDHE-RSA-AES256-GCM-SHA384 6, 92 -- X-Barracuda-URL: https://10.120.88.150:443/cgi-mod/mark.cgi 6, 92 -- X-Virus-Scanned: by bsmtpd at asu.edu 6, 92 -- X-Barracuda-Scan-Msg-Size: 1881 6, 92 -- X-Barracuda-BRTS-Status: 1 6, 92 -- X-Barracuda-Spam-Score: 0.00 6, 92 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using global scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=5.5 tests= 6, 92 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.41635 6, 92 -- Rule breakdown below 6, 92 -- pts rule name description 6, 92 -- ---- ---------------------- -------------------------------------------------- 6, 92 -- Content-Transfer-Encoding: 8bit 6, 92 -- X-MIME-Autoconverted: from base64 to 8bit by microscopy.com id v7448bXt017344 ==============================End of - Headers==============================
I have been using Tungsten filaments in a variety of SEM's and an EPMA for over 35 years and I have never heard of this issue being raised before and never experienced such a phenomenon. My feedback would be similar to what John Mardinly has written below.
Regards
Ray Cantrill
-----Original Message----- X-from: John.Mardinly-at-asu.edu [mailto:John.Mardinly-at-asu.edu] Sent: Friday, 4 August 2017 2:16 PM To: Cantrill, Ray K
Would this vendor by any chance be a vendor of filaments that he wants to sell you? This sounds like a scam. Tungsten evaporates continuously from the filament during its operation. If it is overheated, there can be some melting, but the liquid coalesces into little balls on the ends of the ‘gap’ in the filament. There is nothing that would migrate from the gun to the turbo pump that would cause turbo failure. Pieces of specimen falling off your mounts are the bigger concern.
John Mardinly
} } Remember this posting is most likely not from a Subscriber, so when replying please copy both } miketoalson-at-gmail.com as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: miketoalson-at-gmail.com Name: Mike Toalson } } Organization: NanoImages, LLC } } Title-Subject: [Filtered] Question to Users of Tungsten Filament SEM } } Message: Please help me formulate a stance on a debate about the effects of an SEM's Tungsten } filament breaking when they fail or "burn out". } } One vendor is raising the alarm that if a user waits until a tungsten filament breaks or fails prior } to replacing it, that they run the risk of experiencing a much bigger cleanup issue and even having } "shards" from the filament passing over to the Turbo Pump turbines and causing damage. } } I've seen plenty of burned out Tungsten filaments but never experienced these issues. Of course, } the Wehnelt or Anode can often need a good cleaning which is why most labs have some Pikal or Wenol } paste handy. } } Other than the interruption to replace a filament, has anybody with a Thermonic Tungsten Filament } SEM ever have a major problem from the filament burning out? } } Any thoughts on this matter are much appreciated!! } } Mike Toalson } NanoImages, LLC } }
==============================Original Headers============================== 6, 92 -- From John.Mardinly-at-asu.edu Thu Aug 3 23:08:37 2017 6, 92 -- Received: from bcnete03.asu.edu (bcnete03.asu.edu [13.58.183.106]) 6, 92 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v7448bXt017344 6, 92 -- for {Microscopy-at-Microscopy.com} ; Thu, 3 Aug 2017 23:08:37 -0500 6, 92 -- X-ASG-Debug-ID: 1501819784-0df5cc77ef67d00001-FOsErg 6, 92 -- Received: from mx0b-001d2c01.pphosted.com ([10.120.91.91]) by bcnete03.asu.edu with ESMTP id qYy61Y0GPd4mNla2 (version=TLSv1.2 cipher=ECDHE-RSA-AES256-GCM-SHA384 bits=256 verify=NO); Thu, 03 Aug 2017 21:09:44 -0700 (MST) 6, 92 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu 6, 92 -- X-Barracuda-RBL-Trusted-Forwarder: 10.120.91.91 6, 92 -- Received: from pps.filterd (m0116090.ppops.net [127.0.0.1]) 6, 92 -- by mx0b-001d2c01.pphosted.com (8.16.0.21/8.16.0.21) with SMTP id v7448Gde032700; 6, 92 -- Thu, 3 Aug 2017 21:09:44 -0700 6, 92 -- Received: from nam02-sn1-obe.outbound.protection.outlook.com (mail-sn1nam02lp0022.outbound.protection.outlook.com [216.32.180.22]) 6, 92 -- by mx0b-001d2c01.pphosted.com with ESMTP id 2c4evf85xv-1 6, 92 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES256-SHA384 bits=256 verify=NOT); 6, 92 -- Thu, 03 Aug 2017 21:09:44 -0700 6, 92 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 92 -- d=arizonastateu.onmicrosoft.com; s=selector1-asu-edu; 6, 92 -- h=From:Date:Subject:Message-ID:Content-Type:MIME-Version; 6, 92 -- bh=/ZTU5H233MQVT1Dss5Td+AF3jVIvnCmthjydeY91DIE=; 6, 92 -- b=EHzHclN4lJBzmo7h20EGniWIF56hY3faRMkvxEqumwSZS1WnA14GNW0biaQoP3rXwL6YCd9JYzWhJSVBqybJ4EN2g0BAMho/3ws34eUWk+tBSuGL5ggXvIMyJfK5ZC61wnJuu2aXFxhZgS2OCWw87GlkzMTqjNKrnAdvWTPJpFQ= 6, 92 -- X-Barracuda-RBL-IP: 216.32.180.22 6, 92 -- X-Barracuda-Effective-Source-IP: mail-sn1nam02lp0022.outbound.protection.outlook.com[216.32.180.22] 6, 92 -- X-Barracuda-Apparent-Source-IP: 216.32.180.22 6, 92 -- Received: from CY4PR06MB2904.namprd06.prod.outlook.com (10.175.118.144) by 6, 92 -- CY4PR06MB2902.namprd06.prod.outlook.com (10.175.118.142) with Microsoft SMTP 6, 92 -- Server (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_256_CBC_SHA384_P256) id 6, 92 -- 15.1.1304.22; Fri, 4 Aug 2017 04:09:43 +0000 6, 92 -- Received: from CY4PR06MB2904.namprd06.prod.outlook.com ([10.175.118.144]) by 6, 92 -- CY4PR06MB2904.namprd06.prod.outlook.com ([10.175.118.144]) with mapi id 6, 92 -- 15.01.1304.025; Fri, 4 Aug 2017 04:09:42 +0000 6, 92 -- From: John Mardinly {John.Mardinly-at-asu.edu} 6, 92 -- To: "miketoalson-at-gmail.com" {miketoalson-at-gmail.com} , 6, 92 -- MSA 6, 92 -- {Microscopy-at-Microscopy.com} 6, 92 -- Subject: Re: [Microscopy] viaWWW:Question to Users of Tungsten Filament SEM 6, 92 -- Thread-Topic: [Microscopy] viaWWW:Question to Users of Tungsten Filament SEM 6, 92 -- X-ASG-Orig-Subj: Re: [Microscopy] viaWWW:Question to Users of Tungsten Filament SEM 6, 92 -- Thread-Index: AQHTDML6pkfjUZJ4nkywDcB9O7zR0KJzlXeA 6, 92 -- Date: Fri, 4 Aug 2017 04:09:42 +0000 6, 92 -- Message-ID: {6B6ED2D9-1887-4CF3-AEE4-98A45A66D176-at-asu.edu} 6, 92 -- References: {201708040141.v741fUR8005249-at-microscopy.com} 6, 92 -- In-Reply-To: {201708040141.v741fUR8005249-at-microscopy.com} 6, 92 -- Accept-Language: en-US 6, 92 -- Content-Language: en-US 6, 92 -- X-MS-Has-Attach: 6, 92 -- X-MS-TNEF-Correlator: 6, 92 -- x-originating-ip: [2600:8800:1680:557:f156:3cdb:2aee:fb6d] 6, 92 -- x-ms-publictraffictype: Email 6, 92 -- x-microsoft-exchange-diagnostics: 1;CY4PR06MB2902;7: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 6, 92 -- x-ms-office365-filtering-correlation-id: 253e2689-5944-401b-0274-08d4daeea27a 6, 92 -- x-microsoft-antispam: UriScan:;BCL:0;PCL:0;RULEID:(300000500095)(300135000095)(300000501095)(300135300095)(22001)(300000502095)(300135100095)(2017030254152)(300000503095)(300135400095)(2017052603031)(201703131423075)(201703031133081)(201702281549075)(300000504095)(300135200095)(300000505095)(300135600095)(300000506095)(300135500095);SRVR:CY4PR06MB2902; 6, 92 -- x-ms-traffictypediagnostic: CY4PR06MB2902: 6, 92 -- x-exchange-antispam-report-test: UriScan:(788757137089); 6, 92 -- x-microsoft-antispam-prvs: {CY4PR06MB2902C4C2CBFE8ED13E2785F5E2B60-at-CY4PR06MB2902.namprd06.prod.outlook.com} 6, 92 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:(100000700101)(100105000095)(100000701101)(100105300095)(100000702101)(100105100095)(6040450)(601004)(2401047)(5005006)(8121501046)(93006095)(93001095)(10201501046)(100000703101)(100105400095)(3002001)(6041248)(20161123555025)(20161123558100)(201703131423075)(201702281529075)(201702281528075)(201703061421075)(201703061406153)(20161123564025)(20161123560025)(20161123562025)(6072148)(100000704101)(100105200095)(100000705101)(100105500095);SRVR:CY4PR06MB2902;BCL:0;PCL:0;RULEID:(100000800101)(100110000095)(100000801101)(100110300095)(100000802101)(100110100095)(100000803101)(100110400095)(100000804101)(100110200095)(100000805101)(100110500095);SRVR:CY4PR06MB2902; 6, 92 -- x-forefront-prvs: 0389EDA07F 6, 92 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10009020)(6009001)(39450400003)(39850400002)(39840400002)(39400400002)(39410400002)(39860400002)(189002)(53474002)(199003)(478694002)(6246003)(2906002)(86362001)(25786009)(83716003)(39060400002)(6436002)(97736004)(101416001)(189998001)(75432002)(102836003)(478600001)(38730400002)(3280700002)(53936002)(3660700001)(105586002)(99286003)(106356001)(6116002)(81166006)(2900100001)(6512007)(82746002)(7736002)(305945005)(2950100002)(14454004)(2501003)(88552002)(72206003)(50986999)(8676002)(229853002)(54356999)(77096006)(8936002)(36756003)(5660300001)(81156014)(33656002)(6506006)(6486002)(68736007)(325944009)(76176999);DIR:OUT;SFP:1101;SCL:1;SRVR:CY4PR06MB2902;H:CY4PR06MB2904.namprd06.prod.outlook.com;FPR:;SPF:None;PTR:InfoNoRecords;MX:1;A:1;LANG:en; 6, 92 -- received-spf: None (protection.outlook.com: asu.edu does not designate 6, 92 -- permitted sender hosts) 6, 92 -- spamdiagnosticoutput: 1:99 6, 92 -- spamdiagnosticmetadata: NSPM 6, 92 -- Content-Type: text/plain; charset="utf-8" 6, 92 -- Content-ID: {313AA1F80B194A478F0C1FDBD5FC01DE-at-namprd06.prod.outlook.com} 6, 92 -- MIME-Version: 1.0 6, 92 -- X-OriginatorOrg: asu.edu 6, 92 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 04 Aug 2017 04:09:42.7496 6, 92 -- (UTC) 6, 92 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted 6, 92 -- X-MS-Exchange-CrossTenant-id: 41f88ecb-ca63-404d-97dd-ab0a169fd138 6, 92 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: CY4PR06MB2902 6, 92 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:,, definitions=2017-08-04_01:,, 6, 92 -- signatures=0 6, 92 -- X-Proofpoint-Spam-Details: rule=outbound_notspam policy=outbound score=0 priorityscore=1501 6, 92 -- malwarescore=0 suspectscore=0 phishscore=0 bulkscore=0 spamscore=0 6, 92 -- clxscore=1015 lowpriorityscore=0 impostorscore=0 adultscore=0 6, 92 -- classifier=spam adjust=0 reason=mlx scancount=1 engine=8.0.1-1706020000 6, 92 -- definitions=main-1708040061 6, 92 -- X-Barracuda-Connect: UNKNOWN[10.120.91.91] 6, 92 -- X-Barracuda-Start-Time: 1501819784 6, 92 -- X-Barracuda-Encrypted: ECDHE-RSA-AES256-GCM-SHA384 6, 92 -- X-Barracuda-URL: https://10.120.88.150:443/cgi-mod/mark.cgi 6, 92 -- X-Virus-Scanned: by bsmtpd at asu.edu 6, 92 -- X-Barracuda-Scan-Msg-Size: 1881 6, 92 -- X-Barracuda-BRTS-Status: 1 6, 92 -- X-Barracuda-Spam-Score: 0.00 6, 92 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using global scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=5.5 tests= 6, 92 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.41635 6, 92 -- Rule breakdown below 6, 92 -- pts rule name description 6, 92 -- ---- ---------------------- -------------------------------------------------- 6, 92 -- Content-Transfer-Encoding: 8bit 6, 92 -- X-MIME-Autoconverted: from base64 to 8bit by microscopy.com id v7448bXt017344 ==============================End of - Headers==============================
________________________________
NOTICE - This message and any attached files may contain information that is confidential, legally privileged or proprietary. It is intended only for use by the intended recipient. If you are not the intended recipient or the person responsible for delivering the message to the intended recipient, be advised that you have received this message in error. Any dissemination, copying, use or re-transmission of this message or attachment, or the disclosure of any information therein, is strictly forbidden. BlueScope Steel Limited does not represent or guarantee that this message or attachment is free of errors, virus or interference.
If you have received this message in error please notify the sender immediately and delete the message. Any views expressed in this email are not necessarily the views of BlueScope Steel Limited.
==============================Original Headers============================== 23, 39 -- From Ray.Cantrill-at-bluescopesteel.com Fri Aug 4 00:00:25 2017 23, 39 -- Received: from bslpyrmirn02.vcsc.net.au (bslpyrmirn02.vcsc.net.au [202.53.203.165]) 23, 39 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v7450MwY030181 23, 39 -- for {Microscopy-at-microscopy.com} ; Fri, 4 Aug 2017 00:00:23 -0500 23, 39 -- X-IronPort-AV: E=Sophos;i="5.41,319,1498485600"; 23, 39 -- d="scan'208";a="51156697" 23, 39 -- Received: from STLSYDXCH131.steel.bhpsteel.net (10.88.64.71) by 23, 39 -- STLSYDXCH10.steel.bhpsteel.net (10.88.64.68) with Microsoft SMTP Server (TLS) 23, 39 -- id 14.3.319.2; Fri, 4 Aug 2017 15:00:03 +1000 23, 39 -- Received: from STLSYDXCH131.steel.bhpsteel.net (10.88.64.71) by 23, 39 -- STLSYDXCH131.steel.bhpsteel.net (10.88.64.71) with Microsoft SMTP Server 23, 39 -- (TLS) id 15.0.1178.4; Fri, 4 Aug 2017 15:00:03 +1000 23, 39 -- Received: from STLWOLXCH09.steel.bhpsteel.net (10.81.52.69) by 23, 39 -- STLSYDXCH131.steel.bhpsteel.net (10.88.64.71) with Microsoft SMTP Server 23, 39 -- (TLS) id 15.0.1178.4 via Frontend Transport; Fri, 4 Aug 2017 15:00:03 +1000 23, 39 -- Received: from STLWOLXCH05.steel.bhpsteel.net ([169.254.1.167]) by 23, 39 -- STLWOLXCH09.steel.bhpsteel.net ([10.81.52.69]) with mapi id 14.03.0319.002; 23, 39 -- Fri, 4 Aug 2017 15:00:02 +1000 23, 39 -- From: "Cantrill, Ray K" {Ray.Cantrill-at-bluescopesteel.com} 23, 39 -- To: "Microscopy-at-Microscopy.com" {Microscopy-at-microscopy.com} 23, 39 -- CC: "raycantrill-at-bigpond.com" {raycantrill-at-bigpond.com} 23, 39 -- Subject: RE: [Microscopy] Re: viaWWW:Question to Users of Tungsten Filament 23, 39 -- SEM 23, 39 -- Thread-Topic: [Microscopy] Re: viaWWW:Question to Users of Tungsten Filament 23, 39 -- SEM 23, 39 -- Thread-Index: AQHTDNiFfrkvoZ/Q00ODmFV4QVMbPaJzmWgw 23, 39 -- Date: Fri, 4 Aug 2017 05:00:02 +0000 23, 39 -- Message-ID: {D828204E63B9C446B4E1B4759E509AB613B78594-at-STLWOLXCH05.steel.bhpsteel.net} 23, 39 -- References: {201708040415.v744FZId023718-at-microscopy.com} 23, 39 -- In-Reply-To: {201708040415.v744FZId023718-at-microscopy.com} 23, 39 -- Accept-Language: en-AU, en-US 23, 39 -- Content-Language: en-US 23, 39 -- X-MS-Has-Attach: 23, 39 -- X-MS-TNEF-Correlator: 23, 39 -- x-originating-ip: [10.81.134.131] 23, 39 -- Content-Type: text/plain; charset="utf-8" 23, 39 -- MIME-Version: 1.0 23, 39 -- Content-Transfer-Encoding: 8bit 23, 39 -- X-MIME-Autoconverted: from base64 to 8bit by microscopy.com id v7450MwY030181 ==============================End of - Headers==============================
There would definitely be problems with high voltages in an argon setting, but the question I’ve got is why put the SEM in an argon glove box, instead of using a gas-chamber transfer system between the glovebox and the SEM? The specimen itself is in a vacuum in the SEM, so the environmental sensitivity shouldn’t be an issue.
Justin A. Kraft J. Kraft Microscopy Services, Inc. www.jkraftmicro.com
} On Aug 3, 2017, at 8:33 PM, microscopy.listserver-at-gmail.com wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: miketoalson-at-gmail.com } } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } miketoalson-at-gmail.com as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: miketoalson-at-gmail.com Name: Mike Toalson } } Organization: NanoImages, LLC } } Title-Subject: [Filtered] SEM in Glovebox with ARGON } } Message: Kindly asking the community for some guidance on the use of an SEM or any electronic } instrument for that matter, in a glovebox having an Argon atmosphere. } } After hours of extensive searching, I have not located any experience with 2 reported concerns: } } 1) That there can be issues with arcing of circuit boards or exposed electrical components. } } 2) That motors are subject to overheating - probably only for open motors that are cooled by the } air, but how would a sealed Turbo Molecular Pump fare? } } 3) Argon's breakdown voltage is 20% of that of Air. What effect would this have inside an electronic } device like a instrument or SEM? } } Thank you so much for any advice!! } } Login Host: 47.208.204.209 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 15, 52 -- From microscopy.listserver-at-gmail.com Thu Aug 3 20:24:08 2017 } 15, 52 -- Received: from mail-io0-f178.google.com (mail-io0-f178.google.com [209.85.223.178]) } 15, 52 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v741O72m002585 } 15, 52 -- for {microscopy-at-microscopy.com} ; Thu, 3 Aug 2017 20:24:08 -0500 } 15, 52 -- Received: by mail-io0-f178.google.com with SMTP id m88so1143435iod.2 } 15, 52 -- for {microscopy-at-microscopy.com} ; Thu, 03 Aug 2017 18:25:16 -0700 (PDT) } 15, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 15, 52 -- d=gmail.com; s=20161025; } 15, 52 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 15, 52 -- :in-reply-to:content-language:content-transfer-encoding; } 15, 52 -- bh=TFprenRbZnADAynI5ecA+hWLUbBFtyA+tEIsNErRRJY=; } 15, 52 -- b=M0vymHds3X9p5BnwEI3Mkt1Sj8nIlWNUIhQlLRq8KkWx1DBzEV4kgGOzabafVofOR3 } 15, 52 -- NDlL3O6UjeKqAgKe5DmNRzhx4PEaNcrr4kWwyNsBq80wUZfchEpRAxDFfqXTlTBi7MZ0 } 15, 52 -- LkbQwW0hW/OtYUV1ST/N+t22eZ7ur7Xpjpa2nhbvH2g2wrDkaVmSBwsI4pUcKZR4TceR } 15, 52 -- KzuKUn/bsVmjVVqlekNU/8Q5Khr07Ew2HDLhsPalT8VxZzvoltJsf3He18mQE6vMw0AI } 15, 52 -- QAs+2HJL7kUj7ktRLVcOCrteTPh6kffsw3aABbbMHBTX6RspXLLnPE8hT8eaRXOWBbP0 } 15, 52 -- MUAQ== } 15, 52 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 15, 52 -- d=1e100.net; s=20161025; } 15, 52 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 15, 52 -- :user-agent:mime-version:in-reply-to:content-language } 15, 52 -- :content-transfer-encoding; } 15, 52 -- bh=TFprenRbZnADAynI5ecA+hWLUbBFtyA+tEIsNErRRJY=; } 15, 52 -- b=epDaDhc/fbvZoa4d36d+PDOtn3p0PKqMsr4fkIBctAmwlWG3OXu619jupgwKlXj0bQ } 15, 52 -- ul423STHtxUBVotGyDTvuazFgUhGSX+qRmpMfURoflBb7RCcVE4b3a4KwRmZpgiJc8tN } 15, 52 -- B8+1jUEJgCQBtH2tu9z+zCRotk2cNJJAxkRaFOg6Z27sWvOXVjt8Mhgj9La0wuyd45Ay } 15, 52 -- rDOfOsd4vYcRocR/ZBok/nKlk1WHWcnN9jDLbYH0TL2dHcMb4MgDmhWRQfcRIOTeA0GI } 15, 52 -- 5Tal7qqAOjQ1zPOuVk2UAXsLYt2wZ6fsVAXNyXBdUkdJ6piXLc+a3pTQ3kUBaCubWgNs } 15, 52 -- f0Vg== } 15, 52 -- X-Gm-Message-State: AIVw110fVC4tsuI8cidkKXIcl2WfDgiDacXP/rD/mkh08YwR2fVS9o7K } 15, 52 -- zcmgea68rMqTmkw7xAc= } 15, 52 -- X-Received: by 10.107.40.212 with SMTP id o203mr798026ioo.181.1501809915494; } 15, 52 -- Thu, 03 Aug 2017 18:25:15 -0700 (PDT) } 15, 52 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:9148:ca52:ad23:948c]) } 15, 52 -- by smtp.googlemail.com with ESMTPSA id b14sm153878itb.18.2017.08.03.18.25.14 } 15, 52 -- for {microscopy-at-microscopy.com} } 15, 52 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 15, 52 -- Thu, 03 Aug 2017 18:25:15 -0700 (PDT) } 15, 52 -- Subject: viaWWW:SEM in Glovebox with ARGON } 15, 52 -- References: {201708040059.v740xXsf031443-at-microscopy.com} } 15, 52 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 15, 52 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 15, 52 -- X-Forwarded-Message-Id: {201708040059.v740xXsf031443-at-microscopy.com} } 15, 52 -- Message-ID: {8e0000d6-ccfc-8c6e-df7d-1061fc4a8335-at-gmail.com} } 15, 52 -- Date: Thu, 3 Aug 2017 20:25:14 -0500 } 15, 52 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) } 15, 52 -- Gecko/20100101 Thunderbird/52.2.1 } 15, 52 -- MIME-Version: 1.0 } 15, 52 -- In-Reply-To: {201708040059.v740xXsf031443-at-microscopy.com} } 15, 52 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 15, 52 -- Content-Language: en-US } 15, 52 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
X-from: Jerry Biehler {jerry.biehler-at-gmail.com}
This will probably not end well if you try it. Anything HV will not be happy. I don't think the motors will care too much but the turbo pump will probably need a water cooling loop added. I would look at getting/building a load lock for the SEM and only having that exposes to the inside of the glovebox. -Jerry
} On Aug 3, 2017, at 6:48 PM, microscopy.listserver-at-gmail.com wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: miketoalson-at-gmail.com } } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } miketoalson-at-gmail.com as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: miketoalson-at-gmail.com Name: Mike Toalson } } Organization: NanoImages, LLC } } Title-Subject: [Filtered] SEM in Glovebox with ARGON } } Message: Kindly asking the community for some guidance on the use of an SEM or any electronic } instrument for that matter, in a glovebox having an Argon atmosphere. } } After hours of extensive searching, I have not located any experience with 2 reported concerns: } } 1) That there can be issues with arcing of circuit boards or exposed electrical components. } } 2) That motors are subject to overheating - probably only for open motors that are cooled by the } air, but how would a sealed Turbo Molecular Pump fare? } } 3) Argon's breakdown voltage is 20% of that of Air. What effect would this have inside an electronic } device like a instrument or SEM? } } Thank you so much for any advice!! } } Login Host: 47.208.204.209 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 15, 52 -- From microscopy.listserver-at-gmail.com Thu Aug 3 20:24:08 2017 } 15, 52 -- Received: from mail-io0-f178.google.com (mail-io0-f178.google.com [209.85.223.178]) } 15, 52 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v741O72m002585 } 15, 52 -- for {microscopy-at-microscopy.com} ; Thu, 3 Aug 2017 20:24:08 -0500 } 15, 52 -- Received: by mail-io0-f178.google.com with SMTP id m88so1143435iod.2 } 15, 52 -- for {microscopy-at-microscopy.com} ; Thu, 03 Aug 2017 18:25:16 -0700 (PDT) } 15, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 15, 52 -- d=gmail.com; s=20161025; } 15, 52 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 15, 52 -- :in-reply-to:content-language:content-transfer-encoding; } 15, 52 -- bh=TFprenRbZnADAynI5ecA+hWLUbBFtyA+tEIsNErRRJY=; } 15, 52 -- b=M0vymHds3X9p5BnwEI3Mkt1Sj8nIlWNUIhQlLRq8KkWx1DBzEV4kgGOzabafVofOR3 } 15, 52 -- NDlL3O6UjeKqAgKe5DmNRzhx4PEaNcrr4kWwyNsBq80wUZfchEpRAxDFfqXTlTBi7MZ0 } 15, 52 -- LkbQwW0hW/OtYUV1ST/N+t22eZ7ur7Xpjpa2nhbvH2g2wrDkaVmSBwsI4pUcKZR4TceR } 15, 52 -- KzuKUn/bsVmjVVqlekNU/8Q5Khr07Ew2HDLhsPalT8VxZzvoltJsf3He18mQE6vMw0AI } 15, 52 -- QAs+2HJL7kUj7ktRLVcOCrteTPh6kffsw3aABbbMHBTX6RspXLLnPE8hT8eaRXOWBbP0 } 15, 52 -- MUAQ== } 15, 52 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 15, 52 -- d=1e100.net; s=20161025; } 15, 52 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 15, 52 -- :user-agent:mime-version:in-reply-to:content-language } 15, 52 -- :content-transfer-encoding; } 15, 52 -- bh=TFprenRbZnADAynI5ecA+hWLUbBFtyA+tEIsNErRRJY=; } 15, 52 -- b=epDaDhc/fbvZoa4d36d+PDOtn3p0PKqMsr4fkIBctAmwlWG3OXu619jupgwKlXj0bQ } 15, 52 -- ul423STHtxUBVotGyDTvuazFgUhGSX+qRmpMfURoflBb7RCcVE4b3a4KwRmZpgiJc8tN } 15, 52 -- B8+1jUEJgCQBtH2tu9z+zCRotk2cNJJAxkRaFOg6Z27sWvOXVjt8Mhgj9La0wuyd45Ay } 15, 52 -- rDOfOsd4vYcRocR/ZBok/nKlk1WHWcnN9jDLbYH0TL2dHcMb4MgDmhWRQfcRIOTeA0GI } 15, 52 -- 5Tal7qqAOjQ1zPOuVk2UAXsLYt2wZ6fsVAXNyXBdUkdJ6piXLc+a3pTQ3kUBaCubWgNs } 15, 52 -- f0Vg== } 15, 52 -- X-Gm-Message-State: AIVw110fVC4tsuI8cidkKXIcl2WfDgiDacXP/rD/mkh08YwR2fVS9o7K } 15, 52 -- zcmgea68rMqTmkw7xAc= } 15, 52 -- X-Received: by 10.107.40.212 with SMTP id o203mr798026ioo.181.1501809915494; } 15, 52 -- Thu, 03 Aug 2017 18:25:15 -0700 (PDT) } 15, 52 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:9148:ca52:ad23:948c]) } 15, 52 -- by smtp.googlemail.com with ESMTPSA id b14sm153878itb.18.2017.08.03.18.25.14 } 15, 52 -- for {microscopy-at-microscopy.com} } 15, 52 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 15, 52 -- Thu, 03 Aug 2017 18:25:15 -0700 (PDT) } 15, 52 -- Subject: viaWWW:SEM in Glovebox with ARGON } 15, 52 -- References: {201708040059.v740xXsf031443-at-microscopy.com} } 15, 52 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 15, 52 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 15, 52 -- X-Forwarded-Message-Id: {201708040059.v740xXsf031443-at-microscopy.com} } 15, 52 -- Message-ID: {8e0000d6-ccfc-8c6e-df7d-1061fc4a8335-at-gmail.com} } 15, 52 -- Date: Thu, 3 Aug 2017 20:25:14 -0500 } 15, 52 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) } 15, 52 -- Gecko/20100101 Thunderbird/52.2.1 } 15, 52 -- MIME-Version: 1.0 } 15, 52 -- In-Reply-To: {201708040059.v740xXsf031443-at-microscopy.com} } 15, 52 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 15, 52 -- Content-Language: en-US } 15, 52 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
X-from: Jerry Biehler {jerry.biehler-at-gmail.com}
Filaments are tiny and they tend to "burn out" because tungsten has evaporated away. Only is something catastrophic happened would a filament blow with a lot of debris. None of that debris would ever get to the turbo and even if it did it is so small it would pass right through. Your vendor just wants to sell you a lot of consumables.
-Jerry
} On Aug 3, 2017, at 6:48 PM, microscopy.listserver-at-gmail.com wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: miketoalson-at-gmail.com } } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } miketoalson-at-gmail.com as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: miketoalson-at-gmail.com Name: Mike Toalson } } Organization: NanoImages, LLC } } Title-Subject: [Filtered] Question to Users of Tungsten Filament SEM } } Message: Please help me formulate a stance on a debate about the effects of an SEM's Tungsten } filament breaking when they fail or "burn out". } } One vendor is raising the alarm that if a user waits until a tungsten filament breaks or fails prior } to replacing it, that they run the risk of experiencing a much bigger cleanup issue and even having } "shards" from the filament passing over to the Turbo Pump turbines and causing damage. } } I've seen plenty of burned out Tungsten filaments but never experienced these issues. Of course, } the Wehnelt or Anode can often need a good cleaning which is why most labs have some Pikal or Wenol } paste handy. } } Other than the interruption to replace a filament, has anybody with a Thermonic Tungsten Filament } SEM ever have a major problem from the filament burning out? } } Any thoughts on this matter are much appreciated!! } } Mike Toalson } NanoImages, LLC } } Login Host: 47.208.204.209 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 15, 52 -- From microscopy.listserver-at-gmail.com Thu Aug 3 20:22:33 2017 } 15, 52 -- Received: from mail-io0-f180.google.com (mail-io0-f180.google.com [209.85.223.180]) } 15, 52 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v741MWUr000546 } 15, 52 -- for {microscopy-at-microscopy.com} ; Thu, 3 Aug 2017 20:22:32 -0500 } 15, 52 -- Received: by mail-io0-f180.google.com with SMTP id g71so1066556ioe.5 } 15, 52 -- for {microscopy-at-microscopy.com} ; Thu, 03 Aug 2017 18:23:40 -0700 (PDT) } 15, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 15, 52 -- d=gmail.com; s=20161025; } 15, 52 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 15, 52 -- :in-reply-to:content-language:content-transfer-encoding; } 15, 52 -- bh=v4FPnX2sFhva5ns+3Hr4n+H9Ljr9ZfgXSe6JVTCR2ek=; } 15, 52 -- b=ivDQULcUle3PAXgxH7h47j3LN+ylbBwdydF8FGvvCgIQ5AjQ/Vy4rK0ghOPEYCDAIY } 15, 52 -- Th8zv2McvOjro52JRIIo/5GSX/UehsAgQbgrk030qo0mFEKm7SNdJMArNUSVOl5e3c2B } 15, 52 -- iwoAKX3lbdwcO9At2TEcsZgwnAhCXs8t7p2Soo0sczWcBSUeY1TFLK++lEqvt+F3WxCB } 15, 52 -- VcVBMKo0cIXm5kJSJZC0PvUNBzTSoryubE42ff04awNkPln7tHngYeIzuF4oQYLfFR9W } 15, 52 -- Z7lF3nV059iNN64OpbVrtJd+AqR1AT5Ge2NERe621JQXKGP4VpM7kkxA31yZTYd75ZkM } 15, 52 -- C/tw== } 15, 52 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 15, 52 -- d=1e100.net; s=20161025; } 15, 52 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 15, 52 -- :user-agent:mime-version:in-reply-to:content-language } 15, 52 -- :content-transfer-encoding; } 15, 52 -- bh=v4FPnX2sFhva5ns+3Hr4n+H9Ljr9ZfgXSe6JVTCR2ek=; } 15, 52 -- b=qThQeH2Fqv7cgD/a23MwN0xX2wMnSrsWgAgRVYPyj20dhY19IkWLM07PKzclXf8O3C } 15, 52 -- 3uDGYqZrNhy0Me4YiiIUdpLEekXMPJrp0s8y3tUe9yT/T2/sy9CgSQ3NKvLuy7QSkUD1 } 15, 52 -- d9baf5GRgnZxtwZkJHpuWgJCnclZwlBiYeb3Gb8RVj/Xgqy0SS0JTmD+pQCUkM4PZf7J } 15, 52 -- YhZZf1SVp1dK3FpmTJU1Cjf/fFFGejmGdVMrSofwTQydX60g+50pPjeVWb/+XX6mAcV9 } 15, 52 -- 3nUPQv1huR+CLnEukJUgBihgwH/iV4LirMwM4nswnmwHJdRX65tqeI7i97PJHWC3Oj8E } 15, 52 -- ntaw== } 15, 52 -- X-Gm-Message-State: AHYfb5jKDw2zBzebk0GsPpsTV5h97i2+GrauSDK6lqPY5PWKX62bst1M } 15, 52 -- QFJeo+MIqQwgtSwzk9w= } 15, 52 -- X-Received: by 10.107.170.39 with SMTP id t39mr839237ioe.9.1501809819771; } 15, 52 -- Thu, 03 Aug 2017 18:23:39 -0700 (PDT) } 15, 52 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:28c9:71b1:9c5f:2932]) } 15, 52 -- by smtp.googlemail.com with ESMTPSA id i190sm1319668itb.3.2017.08.03.18.23.38 } 15, 52 -- for {microscopy-at-microscopy.com} } 15, 52 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 15, 52 -- Thu, 03 Aug 2017 18:23:39 -0700 (PDT) } 15, 52 -- Subject: viaWWW:Question to Users of Tungsten Filament SEM } 15, 52 -- References: {201708040114.v741EPTq031824-at-microscopy.com} } 15, 52 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 15, 52 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 15, 52 -- X-Forwarded-Message-Id: {201708040114.v741EPTq031824-at-microscopy.com} } 15, 52 -- Message-ID: {f76964ae-7e9a-2d0b-2997-eb40eaeb5207-at-gmail.com} } 15, 52 -- Date: Thu, 3 Aug 2017 20:23:38 -0500 } 15, 52 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) } 15, 52 -- Gecko/20100101 Thunderbird/52.2.1 } 15, 52 -- MIME-Version: 1.0 } 15, 52 -- In-Reply-To: {201708040114.v741EPTq031824-at-microscopy.com} } 15, 52 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 15, 52 -- Content-Language: en-US } 15, 52 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both maxim.a.beliaev-at-gmail.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] FIB-SEM sample preparation of mature bivalve
Message: What is the best way to prepare bivalve mollusk for the FIB-SEM experiment? We do not want to cut the tissue before fixation in order to preserve internal structure. So, the thickness of the tissue can be up to 2 cm. Additional obstacle for the fixation process is that the tissue is occluded by the shell from one of the sides that we also want to preserve and image. Can prolonged fixation (for 48 h) in glutaraldehyde in cacodylate buffer and sucrose at 4 degrees C be successful? Or if chemical fixation of such big sample would not work, can we use snap-freezing or rapid freeze and freeze-substitution? Can we skip embedding step?
Login Host: 141.76.96.98 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
Hi all, I recently started a postdoc in Europe, and I've just learned that there's some kind of (100 nm-scale) contamination or some other localized inhomogenieties of varying densities in or on the silicon nitride sold by a few manufacturers (e.g. TedPella, SIMPore). In my PhD, I always worked with SPI membranes, which are quite clean. However, it takes quite a while to fill an order for SPI membranes in Europe, as they go through a UK reseller who doesn't keep any SPI products in stock.
I'd like to find a European manufacturer that produces very clean membranes so that I can quickly send new packs to collaborators when necessary (and know that any contamination I see isn't my fault). Does anybody have any experience with any particularly clean membranes that are made (or at least kept in stock) in Europe? Agar Scientific and Micro to Nano both carry grids with the membrane thickess and window size I'm looking for--has anybody noticed any contamination on their grids?
Thanks, Tyler
==============================Original Headers============================== 3, 30 -- From trh-at-uoregon.edu Fri Aug 4 06:33:40 2017 3, 30 -- Received: from smtp.uoregon.edu (cc-smtp4.uoregon.edu [184.171.108.232]) 3, 30 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v74BXdpB016095 3, 30 -- for {microscopy-at-microscopy.com} ; Fri, 4 Aug 2017 06:33:39 -0500 3, 30 -- Received: from mail-io0-f173.google.com (mail-io0-f173.google.com [209.85.223.173]) 3, 30 -- (authenticated bits=0) 3, 30 -- by smtp.uoregon.edu (8.14.4/8.14.4) with ESMTP id v74BYmfC005845 3, 30 -- (version=TLSv1/SSLv3 cipher=AES128-GCM-SHA256 bits=128 verify=NOT) 3, 30 -- for {microscopy-at-microscopy.com} ; Fri, 4 Aug 2017 04:34:48 -0700 3, 30 -- Received: by mail-io0-f173.google.com with SMTP id m88so4903988iod.2 3, 30 -- for {microscopy-at-microscopy.com} ; Fri, 04 Aug 2017 04:34:48 -0700 (PDT) 3, 30 -- X-Gm-Message-State: AHYfb5ivlgsb1h/vUECjRO5uzIGGjss7743DikrfUMTondckIW6Kvu1M 3, 30 -- KPlhAQ89IWJTsTDw1o3+sTJ+NGOiUDp5 3, 30 -- X-Received: by 10.107.15.16 with SMTP id x16mr2082012ioi.288.1501846487967; 3, 30 -- Fri, 04 Aug 2017 04:34:47 -0700 (PDT) 3, 30 -- MIME-Version: 1.0 3, 30 -- Received: by 10.79.129.148 with HTTP; Fri, 4 Aug 2017 04:34:27 -0700 (PDT) 3, 30 -- From: Tyler Harvey {trh-at-uoregon.edu} 3, 30 -- Date: Fri, 4 Aug 2017 13:34:27 +0200 3, 30 -- X-Gmail-Original-Message-ID: {CAPgw9wo1LoC5kXyu3+KmpW1DLjsjnuTZE_Hrb4uDiHTAaq2joA-at-mail.gmail.com} 3, 30 -- Message-ID: {CAPgw9wo1LoC5kXyu3+KmpW1DLjsjnuTZE_Hrb4uDiHTAaq2joA-at-mail.gmail.com} 3, 30 -- Subject: Manufacturers of clean SiN grids in Europe 3, 30 -- To: microscopy-at-microscopy.com 3, 30 -- Content-Type: text/plain; charset="UTF-8" 3, 30 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:,, definitions=2017-08-04_06:,, 3, 30 -- signatures=0 3, 30 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 suspectscore=3 3, 30 -- malwarescore=0 phishscore=0 adultscore=0 bulkscore=0 classifier=spam 3, 30 -- adjust=-40 reason=mlx scancount=1 engine=8.0.1-1706020000 3, 30 -- definitions=main-1708040174 ==============================End of - Headers==============================
Would this vendor happen to have any real estate interest in bridges? Maybe the one in Brooklyn?
More seriously, unless the person who is spreading "alternate facts" is the owner of the company, you might check with the company to see if they know he/she is saying this. I doubt they'd be happy about it.
Philip Oshel Imaging Core Central Michigan University
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both miketoalson-at-gmail.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: miketoalson-at-gmail.com Name: Mike Toalson
Organization: NanoImages, LLC
Title-Subject: [Filtered] Question to Users of Tungsten Filament SEM
Message: Please help me formulate a stance on a debate about the effects of an SEM's Tungsten filament breaking when they fail or "burn out".
One vendor is raising the alarm that if a user waits until a tungsten filament breaks or fails prior to replacing it, that they run the risk of experiencing a much bigger cleanup issue and even having "shards" from the filament passing over to the Turbo Pump turbines and causing damage.
I've seen plenty of burned out Tungsten filaments but never experienced these issues. Of course, the Wehnelt or Anode can often need a good cleaning which is why most labs have some Pikal or Wenol paste handy.
Other than the interruption to replace a filament, has anybody with a Thermonic Tungsten Filament SEM ever have a major problem from the filament burning out?
Any thoughts on this matter are much appreciated!!
Mike Toalson NanoImages, LLC
Login Host: 47.208.204.209 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
All, Did I miss the original post as to why this needs to be done? Does there need to be manipulation of the sample in the glove box whilst imaging? Why add an SEM to a glove box?
My thoughts are heading towards using an ESEM with Argon gas in the chamber. There are nano manipulators too
Chris
Christopher J. Gilpin Ph.D. Director, Life Science Microscopy Facility Campus-wide Coordinator for Electron Microscopy Purdue University Whistler Hall of Agriculture Research, Room S052 170 S. University St West Lafayette, IN 47907 765-494-7750 gilpin-at-purdue.edu lsmf-at-purdue.edu reaches everyone in the facility. http://ag.purdue.edu/arp/Microscopy/Pages/default.aspx
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Friday, August 04, 2017 7:18 AM To: Gilpin, Christopher J {gilpin-at-purdue.edu}
X-from: Jerry Biehler {jerry.biehler-at-gmail.com}
This will probably not end well if you try it. Anything HV will not be happy. I don't think the motors will care too much but the turbo pump will probably need a water cooling loop added. I would look at getting/building a load lock for the SEM and only having that exposes to the inside of the glovebox. -Jerry
} On Aug 3, 2017, at 6:48 PM, microscopy.listserver-at-gmail.com wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://secure-web.cisco.com/16aXH-3dLGhb7Gs7EVaDNwp4EFMSFGxZCvKIMqhkrZIAsvdRx5p2Wb1DHqOqaLZ_uBgdMDkRvCRJ2L59h5S6Y6e-QT7zbjlSR_lBsLq1l0cDxvLoAOgfvrimkai1FSZpbAyafoO2egAR4WCFgwkyF8i5fTfsr3A7Yc9GM2VAniM8jjU_xDKTlWKkit0TFLwrQIOsS5JebdpXqiDLf7g7DrufElsPHcc5Ue5xk4A6IVOIEMGXe_FbbAj0oVYFj7u_MedtUILhArsrdS3XzexZfYmTVItURcla1b1yBNE83ntsLYRFvMnLFXpyVgdnT-e_Iluwj2AD-fr24rKgvwyDbnw/http%3A%2F%2Fwww.microscopy.com%2FMicroscopyListserver } On-Line Help http://secure-web.cisco.com/1UDV0zpyquBE3PSwdJjwEu9DrB5V-Q-YTmdKgBnlJvEnY0hafObZRjywT3IDhX6v2mjLXaMaJ-yfWLJwOOBctne3mGpNDNwo8nDJO3loGD0HDV5wRb93hgAjQm1XYunUiFcmyFJ8d5o7XsRrlw1jpFV-VpM2BOv9qM9DFuh7tvbaADecJND7FuBmIetU5HZ53ishYAYqT3QQOKs_0BMjZOoblKIYL_yY5cn6czUMVCxBjyV_aipalYyyskPGihyUgrhzBPw50CHy_SuDD2yIfj5yfuKfDNcU0ixCQ62hu8u2o-pDKxL0LTFOFcGirGknT3Kq-kpeAtX5BzdE_GggLfw/http%3A%2F%2Fwww.microscopy.com%2FMicroscopyListserver%2FFAQ.html } ---------------------------------------------------------------------------- } } X-from: miketoalson-at-gmail.com } } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://secure-web.cisco.com/1M2z7GdYoFmn5vshYfWTs_Zj4x2jXB_rH5sFE_dUIPgnKYLsGO7RmebANiGsuG9DCjAKU2T7ob-HCbQqTynTzAj77_PDutvStRwpLaYGMWNQDnzNIj0FNj5tAYUpP9OWhf_7U_yiE1EXoLjRM3IcLfCxCoLemdD80ZHXsFp6Xe3Z3lqko8A04JddxZ8t4XSysLoCd-LneArzfNO83TPRY_ygRJvzjeseekXHBXw5dJxOuWUdnqAz-jUmdOitcg6ILs9nzqjI4I6D4QP2iD9JrvesnX7V3hs2AIo80zUzjk5hM9fWfZnpY_RiY48ScXR4e5s5Z0UfAR67O4ywWE63uMw/http%3A%2F%2Fwww.microscopy.com%2FMLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } miketoalson-at-gmail.com as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: miketoalson-at-gmail.com Name: Mike Toalson } } Organization: NanoImages, LLC } } Title-Subject: [Filtered] SEM in Glovebox with ARGON } } Message: Kindly asking the community for some guidance on the use of an SEM or any electronic } instrument for that matter, in a glovebox having an Argon atmosphere. } } After hours of extensive searching, I have not located any experience with 2 reported concerns: } } 1) That there can be issues with arcing of circuit boards or exposed electrical components. } } 2) That motors are subject to overheating - probably only for open motors that are cooled by the } air, but how would a sealed Turbo Molecular Pump fare? } } 3) Argon's breakdown voltage is 20% of that of Air. What effect would this have inside an electronic } device like a instrument or SEM? } } Thank you so much for any advice!! } } Login Host: 47.208.204.209 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 15, 52 -- From microscopy.listserver-at-gmail.com Thu Aug 3 20:24:08 2017 } 15, 52 -- Received: from mail-io0-f178.google.com (mail-io0-f178.google.com [209.85.223.178]) } 15, 52 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v741O72m002585 } 15, 52 -- for {microscopy-at-microscopy.com} ; Thu, 3 Aug 2017 20:24:08 -0500 } 15, 52 -- Received: by mail-io0-f178.google.com with SMTP id m88so1143435iod.2 } 15, 52 -- for {microscopy-at-microscopy.com} ; Thu, 03 Aug 2017 18:25:16 -0700 (PDT) } 15, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 15, 52 -- d=gmail.com; s=20161025; } 15, 52 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 15, 52 -- :in-reply-to:content-language:content-transfer-encoding; } 15, 52 -- bh=TFprenRbZnADAynI5ecA+hWLUbBFtyA+tEIsNErRRJY=; } 15, 52 -- b=M0vymHds3X9p5BnwEI3Mkt1Sj8nIlWNUIhQlLRq8KkWx1DBzEV4kgGOzabafVofOR3 } 15, 52 -- NDlL3O6UjeKqAgKe5DmNRzhx4PEaNcrr4kWwyNsBq80wUZfchEpRAxDFfqXTlTBi7MZ0 } 15, 52 -- LkbQwW0hW/OtYUV1ST/N+t22eZ7ur7Xpjpa2nhbvH2g2wrDkaVmSBwsI4pUcKZR4TceR } 15, 52 -- KzuKUn/bsVmjVVqlekNU/8Q5Khr07Ew2HDLhsPalT8VxZzvoltJsf3He18mQE6vMw0AI } 15, 52 -- QAs+2HJL7kUj7ktRLVcOCrteTPh6kffsw3aABbbMHBTX6RspXLLnPE8hT8eaRXOWBbP0 } 15, 52 -- MUAQ== } 15, 52 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 15, 52 -- d=1e100.net; s=20161025; } 15, 52 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 15, 52 -- :user-agent:mime-version:in-reply-to:content-language } 15, 52 -- :content-transfer-encoding; } 15, 52 -- bh=TFprenRbZnADAynI5ecA+hWLUbBFtyA+tEIsNErRRJY=; } 15, 52 -- b=epDaDhc/fbvZoa4d36d+PDOtn3p0PKqMsr4fkIBctAmwlWG3OXu619jupgwKlXj0bQ } 15, 52 -- ul423STHtxUBVotGyDTvuazFgUhGSX+qRmpMfURoflBb7RCcVE4b3a4KwRmZpgiJc8tN } 15, 52 -- B8+1jUEJgCQBtH2tu9z+zCRotk2cNJJAxkRaFOg6Z27sWvOXVjt8Mhgj9La0wuyd45Ay } 15, 52 -- rDOfOsd4vYcRocR/ZBok/nKlk1WHWcnN9jDLbYH0TL2dHcMb4MgDmhWRQfcRIOTeA0GI } 15, 52 -- 5Tal7qqAOjQ1zPOuVk2UAXsLYt2wZ6fsVAXNyXBdUkdJ6piXLc+a3pTQ3kUBaCubWgNs } 15, 52 -- f0Vg== } 15, 52 -- X-Gm-Message-State: AIVw110fVC4tsuI8cidkKXIcl2WfDgiDacXP/rD/mkh08YwR2fVS9o7K } 15, 52 -- zcmgea68rMqTmkw7xAc= } 15, 52 -- X-Received: by 10.107.40.212 with SMTP id o203mr798026ioo.181.1501809915494; } 15, 52 -- Thu, 03 Aug 2017 18:25:15 -0700 (PDT) } 15, 52 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:9148:ca52:ad23:948c]) } 15, 52 -- by smtp.googlemail.com with ESMTPSA id b14sm153878itb.18.2017.08.03.18.25.14 } 15, 52 -- for {microscopy-at-microscopy.com} } 15, 52 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 15, 52 -- Thu, 03 Aug 2017 18:25:15 -0700 (PDT) } 15, 52 -- Subject: viaWWW:SEM in Glovebox with ARGON } 15, 52 -- References: {201708040059.v740xXsf031443-at-microscopy.com} } 15, 52 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 15, 52 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 15, 52 -- X-Forwarded-Message-Id: {201708040059.v740xXsf031443-at-microscopy.com} } 15, 52 -- Message-ID: {8e0000d6-ccfc-8c6e-df7d-1061fc4a8335-at-gmail.com} } 15, 52 -- Date: Thu, 3 Aug 2017 20:25:14 -0500 } 15, 52 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) } 15, 52 -- Gecko/20100101 Thunderbird/52.2.1 } 15, 52 -- MIME-Version: 1.0 } 15, 52 -- In-Reply-To: {201708040059.v740xXsf031443-at-microscopy.com} } 15, 52 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 15, 52 -- Content-Language: en-US } 15, 52 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Tim_Thomas-at-tkd-inc.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: Tim_Thomas-at-tkd-inc.com Name: Tim Thomas
Organization: PDX Photonics
Title-Subject: [Filtered] Help Requested, HV Issue on Philips 525M SEM
Message: Dear All,
I am the owner of a very nice Philips model 525M SEM. It has a LAB6 source and EDAX. It has been leaking silicon oil from the rubber sealed box containing capacitors that is attached to the side panel inside the instrument for many years. The instrument ran fine until recently. The detector signal started displaying severe noise, then there was a popping sound. The noise went pop, pop, pop, at about 2 or 3 times a second coming from under the instrument. I assume the noise is coming from the HV box under the instrument and not coming from the capacitor box. I shut the instrument down. The ion pump is still running to keep the vacuum up near the source. Your guidance, recommendations, instructions, questions, etc., for helping me bring back to life this wonderful workhorse of an instrument are appreciated.
One additional detail, there are a few small holes on the exterior of the metal around the column. These are starting to shown some kind of foam being extruded from them. The foam is bulging out of the holes by 2 or 3 mm. Not sure if that is significant or not.
Thank you, Tim Thomas Login Host: 73.96.100.81 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
==============================Original Headers============================== 11, 50 -- From microscopy.listserver-at-gmail.com Sun Aug 6 17:59:51 2017 11, 50 -- Received: from mail-it0-f48.google.com (mail-it0-f48.google.com [209.85.214.48]) 11, 50 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v76MxpVW024476 11, 50 -- for {microscopy-at-microscopy.com} ; Sun, 6 Aug 2017 17:59:51 -0500 11, 50 -- Received: by mail-it0-f48.google.com with SMTP id 76so11162402ith.0 11, 50 -- for {microscopy-at-microscopy.com} ; Sun, 06 Aug 2017 16:01:10 -0700 (PDT) 11, 50 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 11, 50 -- d=gmail.com; s=20161025; 11, 50 -- h=subject:references:to:from:message-id:date:user-agent:mime-version 11, 50 -- :in-reply-to:content-transfer-encoding; 11, 50 -- bh=TpyfTUaQ6JiMI5knJ+0udwsi2sPhaTAjqdT+zT2qAYo=; 11, 50 -- b=cL5LzJ/c0+ybhf5V9u7+RtNYpRxvWCZCJvPF5y40iZeo7dYcOu8RtOlIWZO0D4wLMD 11, 50 -- wNhcIVmJYBNq6BrVUXK5kPdNKvVdKMovLleqBHfq+vtEIKj5KDai6vHL5WAQsD5gbrXz 11, 50 -- d1GiZ8WbO4NFB8FGG+TYfMz27clDR9/K4WnGfgBdvYWzkarLLJOn0qfIpryAUmVHNaIU 11, 50 -- fWhFjMlWabsf3RP+S5Cj7r2IpBPWnFxDMKwmKbcOrnUwdnIcnpOMyJ/zp1hPTXV3Dk3Y 11, 50 -- HD0+lU7mZoltBAC6w4tDNbWwees9tL5huNi67vm2DBezrsxEFSx0XJDM4nD7h8Ja1T1R 11, 50 -- Ilcw== 11, 50 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 11, 50 -- d=1e100.net; s=20161025; 11, 50 -- h=x-gm-message-state:subject:references:to:from:message-id:date 11, 50 -- :user-agent:mime-version:in-reply-to:content-transfer-encoding; 11, 50 -- bh=TpyfTUaQ6JiMI5knJ+0udwsi2sPhaTAjqdT+zT2qAYo=; 11, 50 -- b=C44o5wZ06fY4/HILG1c+nXn3wmgT5yu0rGNJgkWN1y63uHVX+2FPqFRRxHxDL4In68 11, 50 -- pi+j63fIy4k3THq94AcSmW+99Y0as9ygEHuZxmKsvmSNQclaN0MheIgGaXmELQVtyjov 11, 50 -- G2Qi1KOls/e6XfNE0Lp2UczJTqPulYV982M0F4YCl6aG8/3DYNIgbxYsFinTPtcqC2Cs 11, 50 -- r2eB3XnTSIcHHzfxeuyJ4Rrd5NJbWpL9TRFafKcx73CK/2c+WnMnsg0kwS24+XF1vSDP 11, 50 -- 8I87R+zjOzUbrrs8C3Ml5aE3Onqmk5xP1WBBpYMEtXcloivGjSic30CY85jA6LTlS11F 11, 50 -- P2ZA== 11, 50 -- X-Gm-Message-State: AIVw1124EH00A/9ux3lZ8oXQZP184UwYUFC5ak8YUYzPz1d5m/CxNq3N 11, 50 -- sLLqlYd3mV+T8vdZ 11, 50 -- X-Received: by 10.36.236.195 with SMTP id g186mr10669804ith.1.1502060469683; 11, 50 -- Sun, 06 Aug 2017 16:01:09 -0700 (PDT) 11, 50 -- Received: from Nestors-MacBookAir-Pro-2014-ElCapitan-OSX-1011.local ([12.226.178.249]) 11, 50 -- by smtp.googlemail.com with ESMTPSA id e92sm2817275itd.0.2017.08.06.16.01.07 11, 50 -- for {microscopy-at-microscopy.com} 11, 50 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 11, 50 -- Sun, 06 Aug 2017 16:01:08 -0700 (PDT) 11, 50 -- Subject: viaWWW:Help Requested, HV Issue on Philips 525M SEM 11, 50 -- References: {201708060152.v761q9RY007240-at-microscopy.com} 11, 50 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 11, 50 -- From: Microscopy Listserver {microscopy.listserver-at-gmail.com} 11, 50 -- X-Forwarded-Message-Id: {201708060152.v761q9RY007240-at-microscopy.com} 11, 50 -- Message-ID: {0204a2a1-d7b8-79a5-e41f-1f86d7816602-at-gmail.com} 11, 50 -- Date: Sun, 6 Aug 2017 18:01:07 -0500 11, 50 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:45.0) 11, 50 -- Gecko/20100101 Thunderbird/45.8.0 11, 50 -- MIME-Version: 1.0 11, 50 -- In-Reply-To: {201708060152.v761q9RY007240-at-microscopy.com} 11, 50 -- Content-Type: text/plain; charset=windows-1252; format=flowed 11, 50 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both book-at-mail.huji.ac.il as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: book-at-mail.huji.ac.il Name: Naomi Book
Organization: Bio-Imaging Unit, The Alexander Silberman Institute of Life Science Edmond Safra Campus (G Ram) The Hebrew University Jerusalem, Israel
Title-Subject: [Filtered] Tecnai 12 TEM Message: We have a FEI Tecnai 12 TEM that is malfunctioning. We suspect one of the power supplies that claims to be a Cherokee PE4130 5V 30A supply, and plugs into a backplane. Cherokee is long gone, and we have no technical info on the TEM, so we now turn to the list for help.
1. Does anyone who has this unit have a service manual that they can share with us?
2 .Is there a retired Tecnai 12 out there whose owners would be willing to provide/sell modules from?
3. Can anyone share experiences (perhaps off-list) concerning experience with service people who are really good with these instruments?
Thanks in advance. Naomi Melamed-Book, Ph.D Bio-Imaging Unit-Director
Login Host: 132.64.60.201 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
X-from: Smith, III, Julian P.S {smithj-at-winthrop.edu}
?? I've never heard of this. Certainly, my JEOL service engineer doesn't recommend anything but replacing the filament after it fails, and our 'scope has a turbopump.
As for "shards"--I doubt it, but don't know.
As for "more cleanup"--well yes, I suppose in that final "bye, cruel world" flash, you get more deposited tungsten. But you're going to clean the wehnelt and anode anyway at each filament change.
Julian
------------------------------------------------------------------------ *From:* microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} *Sent:* Thursday, August 3, 2017 9:35:39 PM *To:* Smith, III, Julian P.S *Subject:* [Microscopy] viaWWW:Question to Users of Tungsten Filament SEM
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
X-from: miketoalson-at-gmail.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both miketoalson-at-gmail.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: miketoalson-at-gmail.com Name: Mike Toalson
Organization: NanoImages, LLC
Title-Subject: [Filtered] Question to Users of Tungsten Filament SEM
Message: Please help me formulate a stance on a debate about the effects of an SEM's Tungsten filament breaking when they fail or "burn out".
One vendor is raising the alarm that if a user waits until a tungsten filament breaks or fails prior to replacing it, that they run the risk of experiencing a much bigger cleanup issue and even having "shards" from the filament passing over to the Turbo Pump turbines and causing damage.
I've seen plenty of burned out Tungsten filaments but never experienced these issues. Of course, the Wehnelt or Anode can often need a good cleaning which is why most labs have some Pikal or Wenol paste handy.
Other than the interruption to replace a filament, has anybody with a Thermonic Tungsten Filament SEM ever have a major problem from the filament burning out?
Any thoughts on this matter are much appreciated!!
Mike Toalson NanoImages, LLC
Login Host: 47.208.204.209 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
It is absolutely correct that putting a microscope in an argon glove box is generally a bad idea due to the low dielectric breakdown voltage of argon. It is, however, of enough interest that multiple vendors of desktop SEMs have investigated it. For instance: https://www.phenom-world.com/accessories/phenom-series/glove-box-compatibility-kit
There are a couple of benefits in addition to reduced complexity associated with multiple load locks and purge chambers.
Argon has a higher pumping speed than house air resulting in reduced pressures and nominally improved service lifetimes of many components. The complete lack of water in the chamber, in principle, allows for the window protecting an SDD (from condensed water) to be removed making lithium detectable by EDS.
-Matt
On Fri, Aug 4, 2017 at 7:26 AM, {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote:
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver On-Line {http://www.microscopy.com/MicroscopyListserverOn-Line} Help http://www.microscopy.com/MicroscopyListserver/FAQ.html {http://www.microscopy.com/MicroscopyListserver/FAQ.html}
X-from: Jerry Biehler {jerry.biehler-at-gmail.com {mailto:jerry.biehler-at-gmail.com} }
This will probably not end well if you try it. Anything HV will not be happy. I don't think the motors will care too much but the turbo pump will probably need a water cooling loop added. I would look at getting/building a load lock for the SEM and only having that exposes to the inside of the glovebox. -Jerry
} On Aug 3, 2017, at 6:48 PM, microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} wrote: } } } } }
---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver {http://www.microscopy.com/MicroscopyListserver} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html {http://www.microscopy.com/MicroscopyListserver/FAQ.html} }
---------------------------------------------------------------------------- } } X-from: miketoalson-at-gmail.com {mailto:miketoalson-at-gmail.com} } } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html {http://www.microscopy.com/MLFormMail.html} }
--------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } miketoalson-at-gmail.com {mailto:miketoalson-at-gmail.com} as well as the Microscopy Listserver }
--------------------------------------------------------------------------- } } Email: miketoalson-at-gmail.com {mailto:miketoalson-at-gmail.com} Name: Mike Toalson } } Organization: NanoImages, LLC } } Title-Subject: [Filtered] SEM in Glovebox with ARGON } } Message: Kindly asking the community for some guidance on the use of an SEM or any electronic } instrument for that matter, in a glovebox having an Argon atmosphere. } } After hours of extensive searching, I have not located any experience with 2 reported concerns: } } 1) That there can be issues with arcing of circuit boards or exposed electrical components. } } 2) That motors are subject to overheating - probably only for open motors that are cooled by the } air, but how would a sealed Turbo Molecular Pump fare? } } 3) Argon's breakdown voltage is 20% of that of Air. What effect would this have inside an electronic } device like a instrument or SEM? } } Thank you so much for any advice!! } } Login Host: 47.208.204.209 } Listserver Email Form V - 20120416 }
the leaking of silicon-oil in older versions of the high voltage parts (like gun, HV main supply and the side-mounted HV box to deliver the HV needed for the cathode tubes for viewing / recording) is a well-known issue at 5x5 electronics.
It even happens at the HV cascade to produce the lower voltages needed for the SE detector cage (used in the SE module on the operator console).
The best you can do is looking for a 5x5 SEM to break down for parts and hope you get a newer (black, not white) version of silicon used in those parts.
If your monitors are still working showing an undisturbed image, the box sitting left-side back down in the electronics might still be OK.
If at small acceleration voltages (ca. 5kV) you get a stable image and the image gets noisy going up in voltage (happens mostly at 20 to 30 kV) your main HV supply (shoebox-size...) is shortening and you need a replacement.
You can try clean the cascades from silicon residue and use a new high-voltage isolating silicon to newly isolate the parts but if there had been a lot of discharging happening in the past in the cascades you might have burned a carbon layer which makes it impossible to use / refurbish these parts.
The "foam" you mentioned is coming from the upper part of the column (containing the cathode assembly) ? There is also silicon used for the heating transformer isolation. If it`s the old version (whitish) it will come out with times... You can only try cleaning the holes with Peth-Ether / Acetone and glue them shut two-component resin. The problem will get worse, since you need to tilt the gun 90 when changing the filament (or you dismount the upper column part and keep it upright all the times during filament change...).
...
The best way would be to look around for parts and exchange the faulty ones. Ask here at the listserver...
If you don`t find parts near you, come back to me and I look around in Germany.
Best wishes,
Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Am 07.08.17 um 01:33 schrieb microscopy.listserver-at-gmail.com: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: Tim_Thomas-at-tkd-inc.com } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both Tim_Thomas-at-tkd-inc.com as well as the } Microscopy Listserver } --------------------------------------------------------------------------- } } Email: Tim_Thomas-at-tkd-inc.com Name: Tim Thomas } } Organization: PDX Photonics } } Title-Subject: [Filtered] Help Requested, HV Issue on Philips 525M SEM } } Message: Dear All, } } I am the owner of a very nice Philips model 525M SEM. It has a LAB6 } source and EDAX. It has been leaking silicon oil from the rubber sealed } box containing capacitors that is attached to the side panel inside the } instrument for many years. The instrument ran fine until recently. The } detector signal started displaying severe noise, then there was a } popping sound. The noise went pop, pop, pop, at about 2 or 3 times a } second coming from under the instrument. I assume the noise is coming } from the HV box under the instrument and not coming from the capacitor } box. I shut the instrument down. The ion pump is still running to keep } the vacuum up near the source. Your guidance, recommendations, } instructions, questions, etc., for helping me bring back to life this } wonderful workhorse of an instrument are appreciated. } } One additional detail, there are a few small holes on the exterior of } the metal around the column. These are starting to shown some kind of } foam being extruded from them. The foam is bulging out of the holes by } 2 or 3 mm. Not sure if that is significant or not. } } Thank you, } Tim Thomas } Login Host: 73.96.100.81 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 11, 50 -- From microscopy.listserver-at-gmail.com Sun Aug 6 17:59:51 2017 } 11, 50 -- Received: from mail-it0-f48.google.com (mail-it0-f48.google.com [209.85.214.48]) } 11, 50 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v76MxpVW024476 } 11, 50 -- for {microscopy-at-microscopy.com} ; Sun, 6 Aug 2017 17:59:51 -0500 } 11, 50 -- Received: by mail-it0-f48.google.com with SMTP id 76so11162402ith.0 } 11, 50 -- for {microscopy-at-microscopy.com} ; Sun, 06 Aug 2017 16:01:10 -0700 (PDT) } 11, 50 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 11, 50 -- d=gmail.com; s=20161025; } 11, 50 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 11, 50 -- :in-reply-to:content-transfer-encoding; } 11, 50 -- bh=TpyfTUaQ6JiMI5knJ+0udwsi2sPhaTAjqdT+zT2qAYo=; } 11, 50 -- b=cL5LzJ/c0+ybhf5V9u7+RtNYpRxvWCZCJvPF5y40iZeo7dYcOu8RtOlIWZO0D4wLMD } 11, 50 -- wNhcIVmJYBNq6BrVUXK5kPdNKvVdKMovLleqBHfq+vtEIKj5KDai6vHL5WAQsD5gbrXz } 11, 50 -- d1GiZ8WbO4NFB8FGG+TYfMz27clDR9/K4WnGfgBdvYWzkarLLJOn0qfIpryAUmVHNaIU } 11, 50 -- fWhFjMlWabsf3RP+S5Cj7r2IpBPWnFxDMKwmKbcOrnUwdnIcnpOMyJ/zp1hPTXV3Dk3Y } 11, 50 -- HD0+lU7mZoltBAC6w4tDNbWwees9tL5huNi67vm2DBezrsxEFSx0XJDM4nD7h8Ja1T1R } 11, 50 -- Ilcw== } 11, 50 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 11, 50 -- d=1e100.net; s=20161025; } 11, 50 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 11, 50 -- :user-agent:mime-version:in-reply-to:content-transfer-encoding; } 11, 50 -- bh=TpyfTUaQ6JiMI5knJ+0udwsi2sPhaTAjqdT+zT2qAYo=; } 11, 50 -- b=C44o5wZ06fY4/HILG1c+nXn3wmgT5yu0rGNJgkWN1y63uHVX+2FPqFRRxHxDL4In68 } 11, 50 -- pi+j63fIy4k3THq94AcSmW+99Y0as9ygEHuZxmKsvmSNQclaN0MheIgGaXmELQVtyjov } 11, 50 -- G2Qi1KOls/e6XfNE0Lp2UczJTqPulYV982M0F4YCl6aG8/3DYNIgbxYsFinTPtcqC2Cs } 11, 50 -- r2eB3XnTSIcHHzfxeuyJ4Rrd5NJbWpL9TRFafKcx73CK/2c+WnMnsg0kwS24+XF1vSDP } 11, 50 -- 8I87R+zjOzUbrrs8C3Ml5aE3Onqmk5xP1WBBpYMEtXcloivGjSic30CY85jA6LTlS11F } 11, 50 -- P2ZA== } 11, 50 -- X-Gm-Message-State: AIVw1124EH00A/9ux3lZ8oXQZP184UwYUFC5ak8YUYzPz1d5m/CxNq3N } 11, 50 -- sLLqlYd3mV+T8vdZ } 11, 50 -- X-Received: by 10.36.236.195 with SMTP id g186mr10669804ith.1.1502060469683; } 11, 50 -- Sun, 06 Aug 2017 16:01:09 -0700 (PDT) } 11, 50 -- Received: from Nestors-MacBookAir-Pro-2014-ElCapitan-OSX-1011.local ([12.226.178.249]) } 11, 50 -- by smtp.googlemail.com with ESMTPSA id e92sm2817275itd.0.2017.08.06.16.01.07 } 11, 50 -- for {microscopy-at-microscopy.com} } 11, 50 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 11, 50 -- Sun, 06 Aug 2017 16:01:08 -0700 (PDT) } 11, 50 -- Subject: viaWWW:Help Requested, HV Issue on Philips 525M SEM } 11, 50 -- References: {201708060152.v761q9RY007240-at-microscopy.com} } 11, 50 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 11, 50 -- From: Microscopy Listserver {microscopy.listserver-at-gmail.com} } 11, 50 -- X-Forwarded-Message-Id: {201708060152.v761q9RY007240-at-microscopy.com} } 11, 50 -- Message-ID: {0204a2a1-d7b8-79a5-e41f-1f86d7816602-at-gmail.com} } 11, 50 -- Date: Sun, 6 Aug 2017 18:01:07 -0500 } 11, 50 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:45.0) } 11, 50 -- Gecko/20100101 Thunderbird/45.8.0 } 11, 50 -- MIME-Version: 1.0 } 11, 50 -- In-Reply-To: {201708060152.v761q9RY007240-at-microscopy.com} } 11, 50 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 11, 50 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
==============================Original Headers============================== 21, 30 -- From diller-at-stefan-diller.com Mon Aug 7 04:14:47 2017 21, 30 -- Received: from mailout022.rox.net (mailout022.rox.net [212.63.85.222]) 21, 30 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v779EkqH000784 21, 30 -- for {microscopy-at-microscopy.com} ; Mon, 7 Aug 2017 04:14:47 -0500 21, 30 -- Received: from localhost ([127.0.0.1]) 21, 30 -- by mailout02.rox.net with esmtp (Exim 4.80) 21, 30 -- (envelope-from {diller-at-stefan-diller.com} ) 21, 30 -- id 1dee8s-000F5h-2B; Mon, 07 Aug 2017 11:16:06 +0200 21, 30 -- Received: from p5082a6b5.dip0.t-ipconnect.de ([80.130.166.181] helo=mac-pro.local) 21, 30 -- by mailout02.rox.net with esmtpsa (TLSv1.2:DHE-RSA-AES128-SHA:128) 21, 30 -- (Exim 4.80) 21, 30 -- (envelope-from {diller-at-stefan-diller.com} ) 21, 30 -- id 1dee8r-000F1g-E5; Mon, 07 Aug 2017 11:16:05 +0200 21, 30 -- Subject: Re: [Microscopy] viaWWW:Help Requested, HV Issue on Philips 525M SEM 21, 30 -- To: "Microscopy-at-microscopy.com" {microscopy-at-microscopy.com} , 21, 30 -- Tim_Thomas-at-tkd-inc.com 21, 30 -- References: {201708062333.v76NXjmS004958-at-microscopy.com} 21, 30 -- From: stefan diller {diller-at-stefan-diller.com} 21, 30 -- Message-ID: {ba8bb1d2-4a17-9ea4-cea9-8fe54d54b7ec-at-stefan-diller.com} 21, 30 -- Date: Mon, 7 Aug 2017 11:16:03 +0200 21, 30 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) 21, 30 -- Gecko/20100101 Thunderbird/52.2.1 21, 30 -- MIME-Version: 1.0 21, 30 -- In-Reply-To: {201708062333.v76NXjmS004958-at-microscopy.com} 21, 30 -- Content-Type: text/plain; charset=windows-1252; format=flowed 21, 30 -- Content-Language: en-GB 21, 30 -- X-Envelope-From: {diller-at-stefan-diller.com} 21, 30 -- X-Scanned-By: rockenstein AG 21, 30 -- Content-Transfer-Encoding: 8bit 21, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v779EkqH000784 ==============================End of - Headers==============================
Thank you so much to all in the community for providing some great comments and suggestions.
Some have asked why was this being asked about? Our company is a distributor of tabletop SEM and we have a project where a customer with another type SEM wishes to have a system inside an argon filled glovebox for analyzing samples that cannot be exposed to air.Like many of you, my first thought was “why not use a transfer device like the Quorum accessory?”.However the type of SEM being considered (not ours) has no external port so the solution for them is to put the entire compact SEM in the glovebox with a special modification kit.
Our system has 2 “boxes” with extendable cables to the electronics box so only the column and HV supply would be in the glovebox. Thus,I was curious about experiences with this.I do like the one idea of using a cold plate to keep the components from overheating.More study is needed though and the use of a transfer system seems less complicated for sure however, our system only has one side port that is normally used for an EDS but we are looking into modifications.
Curious if any of you have looked at using something like the small transfer device shown here with the “rupture” film?My first concern would be does the film stay intact after splitting open but the paper describes using elastic films that are not prone to this.It seems like a simple and elegant way to move a sample from glovebox to SEM without the need of an Airlock or complex shuttle.
On Sun, Aug 6, 2017 at 4:38 PM, {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote:
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver On-Line {http://www.microscopy.com/MicroscopyListserver On-Line} Help http://www.microscopy.com/MicroscopyListserver/FAQ.html {http://www.microscopy.com/MicroscopyListserver/FAQ.html}
X-from: Matt Jobbins {mmjobbins-at-gmail.com {mailto:mmjobbins-at-gmail.com} }
It is absolutely correct that putting a microscope in an argon glove box is generally a bad idea due to the low dielectric breakdown voltage of argon. It is, however, of enough interest that multiple vendors of desktop SEMs have investigated it. For instance:
There are a couple of benefits in addition to reduced complexity associated with multiple load locks and purge chambers.
Argon has a higher pumping speed than house air resulting in reduced pressures and nominally improved service lifetimes of many components. The complete lack of water in the chamber, in principle, allows for the window protecting an SDD (from condensed water) to be removed making lithium detectable by EDS.
-Matt
On Fri, Aug 4, 2017 at 7:26 AM, {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} {mailto:microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } } wrote:
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver {http://www.microscopy.com/MicroscopyListserver} On-Line {http://www.microscopy.com/MicroscopyListserverOn-Line {http://www.microscopy.com/MicroscopyListserverOn-Line} } Help http://www.microscopy.com/MicroscopyListserver/FAQ.html {http://www.microscopy.com/MicroscopyListserver/FAQ.html} {http://www.microscopy.com/MicroscopyListserver/FAQ.html {http://www.microscopy.com/MicroscopyListserver/FAQ.html} }
X-from: Jerry Biehler {jerry.biehler-at-gmail.com {mailto:jerry.biehler-at-gmail.com} {mailto:jerry.biehler-at-gmail.com {mailto:jerry.biehler-at-gmail.com} } }
This will probably not end well if you try it. Anything HV will not be happy. I don't think the motors will care too much but the turbo pump will probably need a water cooling loop added. I would look at getting/building a load lock for the SEM and only having that exposes to the inside of the glovebox. -Jerry
} On Aug 3, 2017, at 6:48 PM, microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} {mailto:microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote: } } } } }
---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver {http://www.microscopy.com/MicroscopyListserver} {http://www.microscopy.com/MicroscopyListserver {http://www.microscopy.com/MicroscopyListserver} } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html {http://www.microscopy.com/MicroscopyListserver/FAQ.html} {http://www.microscopy.com/MicroscopyListserver/FAQ.html {http://www.microscopy.com/MicroscopyListserver/FAQ.html} } }
---------------------------------------------------------------------------- } } X-from: miketoalson-at-gmail.com {mailto:miketoalson-at-gmail.com} {mailto:miketoalson-at-gmail.com {mailto:miketoalson-at-gmail.com} } } } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html {http://www.microscopy.com/MLFormMail.html} {http://www.microscopy.com/MLFormMail.html {http://www.microscopy.com/MLFormMail.html} } }
--------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } miketoalson-at-gmail.com {mailto:miketoalson-at-gmail.com} {mailto:miketoalson-at-gmail.com {mailto:miketoalson-at-gmail.com} } as well as the Microscopy Listserver }
--------------------------------------------------------------------------- } } Email: miketoalson-at-gmail.com {mailto:miketoalson-at-gmail.com} {mailto:miketoalson-at-gmail.com {mailto:miketoalson-at-gmail.com} } Name: Mike Toalson } } Organization: NanoImages, LLC } } Title-Subject: [Filtered] SEM in Glovebox with ARGON } } Message: Kindly asking the community for some guidance on the use of an SEM or any electronic } instrument for that matter, in a glovebox having an Argon atmosphere. } } After hours of extensive searching, I have not located any experience with 2 reported concerns: } } 1) That there can be issues with arcing of circuit boards or exposed electrical components. } } 2) That motors are subject to overheating - probably only for open motors that are cooled by the } air, but how would a sealed Turbo Molecular Pump fare? } } 3) Argon's breakdown voltage is 20% of that of Air. What effect would this have inside an electronic } device like a instrument or SEM? } } Thank you so much for any advice!! } } Login Host: 47.208.204.209 } Listserver Email Form V - 20120416 }
That said, I have heard of and seen this. While visiting a lab running a TEM, I was shown a filament with the top blown off and told it happened because there had been a high voltage surge to the instrument, and tungsten filament debris was found in the scope. TEM, not SEM. I had just talked about this with a lab mate the might before this question was posted on the list server.
Never otherwise heard of it.
KP
On Sunday, August 6, 2017, {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote:
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver On-Line {http://www.microscopy.com/MicroscopyListserver On-Line} Help http://www.microscopy.com/MicroscopyListserver/FAQ.html {http://www.microscopy.com/MicroscopyListserver/FAQ.html}
X-from: Smith, III, Julian P.S {smithj-at-winthrop.edu {javascript:;} }
?? I've never heard of this. Certainly, my JEOL service engineer doesn't recommend anything but replacing the filament after it fails, and our 'scope has a turbopump.
As for "shards"--I doubt it, but don't know.
As for "more cleanup"--well yes, I suppose in that final "bye, cruel world" flash, you get more deposited tungsten. But you're going to clean the wehnelt and anode anyway at each filament change.
Julian
------------------------------------------------------------------------ *From:* microscopy.listserver-at-gmail.com {javascript:;} {microscopy.listserver-at-gmail.com {javascript:;} } *Sent:* Thursday, August 3, 2017 9:35:39 PM *To:* Smith, III, Julian P.S *Subject:* [Microscopy] viaWWW:Question to Users of Tungsten Filament SEM
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver On-Line {http://www.microscopy.com/MicroscopyListserver On-Line} Help http://www.microscopy.com/MicroscopyListserver/FAQ.html {http://www.microscopy.com/MicroscopyListserver/FAQ.html}
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html {http://www.microscopy.com/MLFormMail.html}
--------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both miketoalson-at-gmail.com {javascript:;} as well as the Microscopy Listserver
Email: miketoalson-at-gmail.com {javascript:;} Name: Mike Toalson
Organization: NanoImages, LLC
Title-Subject: [Filtered] Question to Users of Tungsten Filament SEM
Message: Please help me formulate a stance on a debate about the effects of an SEM's Tungsten filament breaking when they fail or "burn out".
One vendor is raising the alarm that if a user waits until a tungsten filament breaks or fails prior to replacing it, that they run the risk of experiencing a much bigger cleanup issue and even having "shards" from the filament passing over to the Turbo Pump turbines and causing damage.
I've seen plenty of burned out Tungsten filaments but never experienced these issues. Of course, the Wehnelt or Anode can often need a good cleaning which is why most labs have some Pikal or Wenol paste handy.
Other than the interruption to replace a filament, has anybody with a Thermonic Tungsten Filament SEM ever have a major problem from the filament burning out?
Any thoughts on this matter are much appreciated!!
Mike Toalson NanoImages, LLC
Login Host: 47.208.204.209 Listserver Email Form V - 20120416
From jeverett397-at-gmail.com Tue Aug 8 16:49:17 2017 Return-Path: {jeverett397-at-gmail.com} Received: from gmail.com (mail.dongnamdc.com [222.97.39.100] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v78LnEof003582 for {microscopylistserverarchive6-at-microscopy.com} ; Tue, 8 Aug 2017 16:49:16 -0500 Message-ID: {309301d3109f$ba39a1e0$138f65be-at-jeverett397} Reply-To: "Miles Molina " {jeverett397-at-gmail.com}
X-from: annett.thogersen-at-sintef.no
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both annett.thogersen-at-sintef.no as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] TEM cross-sectional glue
Message: Dear Colleagues,
I am looking for a TEM cross-sectional glue that do not need any heat for hardening, is easy to mix, and will have minimal contamination in the TEM. I have one that I use now, but unfortunately it softens in acetone. Does anyone have some good ideas on what glue I can use? I cannot expose my samples to very much heat. Thank you, Annett Thgersen
Login Host: 78.91.103.180 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both bonnie_salyer-at-tedpella.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Microscopy Career Opportunities
Message: Ted Pella, Inc. is a worldwide leader in the microscopy supplies and specimen preparation instrumentation field seeking qualified applicants for: Materials Science Product Specialist Microscopy International Sales Manager
For more information visit our web site at www.tedpella.com. To apply please send a letter of interest and resume to human_resources-at-tedpella.com Live and work in beautiful Redding, California surrounded by some of Northern Californias most beautiful natural attractions, and in the midst of small city cultural entertainment, history, dining and shopping. No more long commutes to work, and only minutes away from amazing waterfall hikes, cycling trails and nationally-ranked fishing. www.visitredding.com
Login Host: 12.7.209.242 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mj_iqbal-at-yahoo as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: mj_iqbal-at-yahoo Name: M Javed Iqbal
Organization: NIBGE Title-Subject: [Filtered] Electron Beam Brightness issue Jeol JEM-1010 after filament change, suggestions required
Message: Hello Dear all suggestions/ help is required regarding the electron microscope filament beam which is not sharp/bright enough (shadow of the fixed aperture) after I replaced the fused filament with new one, in past we have done this practice multiple times without any issue. I have done all the possible filament distance adjusting options with the wehnelt cap/cover but no major difference in results more over obtaining filament saturation point did not yield a converged single very sharp point and when we De-saturate the filament by turning the filament knob counter clockwise its not producing the typical image of two balanced half crescents holing a round spot inside.without engaging any lens aperture the shadow of fixed aperture in having a hallow zone like double spot with slight missing the full overlap Is it possible to post an image or video clip of the same for better understanding ?
Muhammad javed Iqbal TEM Core Facilities Lab NIBGE Pakistan
Login Host: 202.59.89.36 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both andy.stewart-at-ul.ie as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: andy.stewart-at-ul.ie Name: Andy Stewart
Organization: University of Limerick
Title-Subject: [Filtered] 3 year postdoctoral position available
Message: Postdoctoral position in electron microscopy of pharmaceutical crystals. A 3-year postdoctoral position in TEM is available at the University of Limerick, Ireland, as part of the H2020 award MagnaPharm (https://www.magnapharm.com) the aim of this researchproject is to control polymorphism in pharmaceutical crystal growth via magnetic fields, and to solve crystal structure and morphology of these compounds using electron imaging and diffraction methods as well as to build a matrix of machine conditions for data evaluation on organic crystals.
Work will be carried out using a double corrected, monochromated Titan Themis, equipped with EDS and EELS, K2-IS and Oneview detectors, as well as in-situ holders (DENS lightning double tilt , DENS ocean, DENS atmosphere, Fischione cryo-transfer holder.) Please apply here: http://www.ul.ie Vacancies Job ID: 023368
Closing date is the 31th of August 2017. For further information please contact Ursel.Bangert-at-ul.ie or Andy.Stewart-at-ul.ie
Login Host: 193.1.100.65 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
==============================Original Headers============================== 12, 51 -- From microscopy.listserver-at-gmail.com Fri Aug 11 06:29:31 2017 12, 51 -- Received: from mail-it0-f52.google.com (mail-it0-f52.google.com [209.85.214.52]) 12, 51 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v7BBTV3W003513 12, 51 -- for {microscopy-at-microscopy.com} ; Fri, 11 Aug 2017 06:29:31 -0500 12, 51 -- Received: by mail-it0-f52.google.com with SMTP id m34so25784936iti.1 12, 51 -- for {microscopy-at-microscopy.com} ; Fri, 11 Aug 2017 04:31:07 -0700 (PDT) 12, 51 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 12, 51 -- d=gmail.com; s=20161025; 12, 51 -- h=subject:references:to:from:message-id:date:user-agent:mime-version 12, 51 -- :in-reply-to:content-transfer-encoding; 12, 51 -- bh=R6fcDWcqlwhp/k2acxz5sya4fAiavz5wU0xB6XzxaHg=; 12, 51 -- b=d764ucVe+uLSDiNmebjAFeZi1HlpcshTH77n+xxAkxhT8I2CSzfZ2+Hf+8EJW7clXs 12, 51 -- 5VYoNtQi1lTv6Ot7wAK2FqKVWF1CzwL8jtTii6+Iph9lq7dCx/ZXhKRNntk4oGWvSxNX 12, 51 -- Tmfb7aNT/1v4X7GamPvlVki3woqPnAwMGgOvBQo7SUI5FNhGypYfitNPlvhO3OFhdxfi 12, 51 -- 1exxTEY25ffnvet0Q6jaAs0Jq0+Ayk9TotGPTIwSLujA5YR1TixpOCvLAOzZp9AA6JQK 12, 51 -- oyMwrnR5lixsbAXGRcCXFwli5g3rxNvyxV/lNOyvPFuQ94lnLga2lC6Cl9VgqPQjD6EY 12, 51 -- 3M8Q== 12, 51 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 12, 51 -- d=1e100.net; s=20161025; 12, 51 -- h=x-gm-message-state:subject:references:to:from:message-id:date 12, 51 -- :user-agent:mime-version:in-reply-to:content-transfer-encoding; 12, 51 -- bh=R6fcDWcqlwhp/k2acxz5sya4fAiavz5wU0xB6XzxaHg=; 12, 51 -- b=F8lD7KjRiwdkKA814MfTBc4H9R0TvBX5RuzNRsIYzOetxI2s0gvc4/3E2zQ4xxvcaT 12, 51 -- rl+DPE1FEtU56eBArYYUlLnweH6Fj3HS7SaASgdEVwPJrzQQ4WFY7/AdhhVeQU144xaS 12, 51 -- JjR7GB02Qzdd55UfrNKPm3L3riwiWV2aeRFwPqHc28uphb2UEHCzanCq1N4Zlyd4jsPL 12, 51 -- U6Ul/78kTYBADGXN8Ckyr7Q/D2T5uehYOThPkBHqEqsQ01fWTwD+NRL4zZqCPMYStCZO 12, 51 -- /pXutkh0aWLz26MUWPtVgi1X+/AiwbE215784Rp1VRBx44zKfAvTKn1mg+AiWugZNftY 12, 51 -- edCg== 12, 51 -- X-Gm-Message-State: AHYfb5hgl0NlLTmNOiHfQ1CYpAiPwyk9KbSKuw0t2IkVhq+GTHGvWqYv 12, 51 -- j86WWWrRZvTynHme 12, 51 -- X-Received: by 10.36.155.136 with SMTP id o130mr14539467itd.104.1502451066729; 12, 51 -- Fri, 11 Aug 2017 04:31:06 -0700 (PDT) 12, 51 -- Received: from Nestors-MacBookAir-Pro-2014-ElCapitan-OSX-1011.local ([12.226.178.249]) 12, 51 -- by smtp.googlemail.com with ESMTPSA id f70sm350977ita.1.2017.08.11.04.31.06 12, 51 -- for {microscopy-at-microscopy.com} 12, 51 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 12, 51 -- Fri, 11 Aug 2017 04:31:06 -0700 (PDT) 12, 51 -- Subject: viaWWW:3 year postdoctoral position available University of Limerick, 12, 51 -- Ireland 12, 51 -- References: {201708101126.v7ABQanr027416-at-microscopy.com} 12, 51 -- To: microscopy-at-microscopy.com 12, 51 -- From: Microscopy Listserver {microscopy.listserver-at-gmail.com} 12, 51 -- X-Forwarded-Message-Id: {201708101126.v7ABQanr027416-at-microscopy.com} 12, 51 -- Message-ID: {f07276f2-9a04-dc97-b99c-f07c12ee4c9f-at-gmail.com} 12, 51 -- Date: Fri, 11 Aug 2017 06:31:05 -0500 12, 51 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:45.0) 12, 51 -- Gecko/20100101 Thunderbird/45.8.0 12, 51 -- MIME-Version: 1.0 12, 51 -- In-Reply-To: {201708101126.v7ABQanr027416-at-microscopy.com} 12, 51 -- Content-Type: text/plain; charset=windows-1252; format=flowed 12, 51 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jshields-at-uga.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: jshields-at-uga.edu Name: John P Shields
Organization: University of Georgia
Title-Subject: [Filtered] October Biological TEM workshop
Message: Biological TEM Workshop
This intensive, three-day workshop will provide a practical and basic theoretical introduction to the Transmission Electron Microscope and biological sample preparation techniques. Each day will consist of lecture, discussion and hands-on training led by GEM staff. What: Anyone requiring training on TEM and biological sample preparation. The workshop will be limited to 6 participants based on the availability of equipment. When: Monday through Wednesday, October 25-27, 2017, 8am-5pm each day (lunch is provided)
Where: 154 Barrow Hall, University of Georgia, Athens, GA 30602
Registration: Contact John Shields (jpshield-at-uga.edu) for more information and to sign up. Registration requires iLab account through the GEM website. https://uga.ilabsolutions.com/account/login
Deadline: October 15, 2017
Login Host: 198.137.20.67 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
There is an epoxy designed for plugging leaks inUHV systems called “Torr Seal” that is incredible. The only drawback is that it is quite viscous and does not form thin joints. However, it is filled with ceramic particles so it does not mill preferentially. It cures at room temperature, has very strong adhesion and does not contaminate. According to web, still manufactured by Varian and available through Kurt Lesker and others.
John Mardinly
} On Aug 11, 2017, at 4:48 AM, microscopy.listserver-at-gmail.com wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.com_MicroscopyListserver&d=DwIB-g&c=l45AxH-kUV29SRQusp9vYR0n1GycN4_2jInuKy6zbqQ&r=VLPJ8OE-c_C6joGeE1ftlvxMmQPq9N6mpKZONBRt90E&m=Xqo77yEPl5uebwcYQX6kXPeB3QatmpGEdAQFd-p21pw&s=YasAbG-7_cd1NUviH5V5bDXoSK1UZGj-hW66Pedyl28&e= } On-Line Help https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.com_MicroscopyListserver_FAQ.html&d=DwIB-g&c=l45AxH-kUV29SRQusp9vYR0n1GycN4_2jInuKy6zbqQ&r=VLPJ8OE-c_C6joGeE1ftlvxMmQPq9N6mpKZONBRt90E&m=Xqo77yEPl5uebwcYQX6kXPeB3QatmpGEdAQFd-p21pw&s=7j57uMg2tmLpVRw7B_OXNbgWQYryOb2wY2mtKHLf9WU&e= } ---------------------------------------------------------------------------- } } X-from: annett.thogersen-at-sintef.no } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } https://urldefense.proofpoint.com/v2/url?u=http-3A__microscopy.com_MicroscopyListserver_MLFormMail.html&d=DwIB-g&c=l45AxH-kUV29SRQusp9vYR0n1GycN4_2jInuKy6zbqQ&r=VLPJ8OE-c_C6joGeE1ftlvxMmQPq9N6mpKZONBRt90E&m=Xqo77yEPl5uebwcYQX6kXPeB3QatmpGEdAQFd-p21pw&s=FbYafrxEtkqI7FOGIcit4TM8yPWjzZHtxrY8TvkRP8g&e= } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both annett.thogersen-at-sintef.no as well as the } Microscopy Listserver } --------------------------------------------------------------------------- } } Email: annett.thogersen-at-sintef.no Name: Annett Thøgersen } } Organization: SINTEF } } Title-Subject: [Filtered] TEM cross-sectional glue } } Message: Dear Colleagues, } } I am looking for a TEM cross-sectional glue that do not need any heat } for hardening, is easy to mix, and will have minimal contamination in } the TEM. I have one that I use now, but unfortunately it softens in } acetone. Does anyone have some good ideas on what glue I can use? I } cannot expose my samples to very much heat. } Thank you, } Annett Thøgersen } } Login Host: 78.91.103.180 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 10, 50 -- From microscopy.listserver-at-gmail.com Fri Aug 11 06:27:21 2017 } 10, 50 -- Received: from mail-it0-f44.google.com (mail-it0-f44.google.com [209.85.214.44]) } 10, 50 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v7BBRLmq000450 } 10, 50 -- for {microscopy-at-microscopy.com} ; Fri, 11 Aug 2017 06:27:21 -0500 } 10, 50 -- Received: by mail-it0-f44.google.com with SMTP id 76so25760458ith.0 } 10, 50 -- for {microscopy-at-microscopy.com} ; Fri, 11 Aug 2017 04:28:57 -0700 (PDT) } 10, 50 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 10, 50 -- d=gmail.com; s=20161025; } 10, 50 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 10, 50 -- :in-reply-to:content-transfer-encoding; } 10, 50 -- bh=l7YWH6wy+lp7x5P7btPs8KynWsXUUHyLl9Ztba8UZ44=; } 10, 50 -- b=abcP64/KXD6U48G9L1KU+FZNDNf049Nyec0YKvCrWyCCMcG7XXIG1CQoAUSWX+vKqt } 10, 50 -- 45UcmjyRUpNdFaaxkdjsIBc/2B/n1roo6uPp5Q+vkqf34480FWGtklR01cauee52HoyE } 10, 50 -- grDOyZa2cCtb8M4jALvVgbX/HeQy5B/3K8r2EwyHkYsSuUD0OkDf2C30a/DHYgzlA74X } 10, 50 -- 15ri/y453b0D5FE3vzPPWL+fdjOoDdbjDhDy5OvePq/PgrctFt2+QEj3qGJM5DvQEX9x } 10, 50 -- Bc2lBY8S4ZUXBsqrCTMSDyDc0X4kMhyWxPpSmYy4B7TWkggbGZu3xDGKxLFcHzi3ff0s } 10, 50 -- wfCA== } 10, 50 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 10, 50 -- d=1e100.net; s=20161025; } 10, 50 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 10, 50 -- :user-agent:mime-version:in-reply-to:content-transfer-encoding; } 10, 50 -- bh=l7YWH6wy+lp7x5P7btPs8KynWsXUUHyLl9Ztba8UZ44=; } 10, 50 -- b=WiK+4nJPPK5SSYVsDuSwAtyStr3Tq8C5g+R2gVQB+HcmA9IOpROrxszaxo2zW1FFdg } 10, 50 -- ExCEW62QNbLjCHsPCxOb/faCgAODtrT2r85isFno0iybiFodLhOi40uqvGS5h0hvOi6E } 10, 50 -- rdMtyMDCQr+WM6ofxSF1CZHwT+NI39plEmvJwOwfJ4qUPVVHk3jMTU2Jxe81zaLKnBEo } 10, 50 -- /kf7XtgFHUs8Tspg2sZjDUIwq5j6gCTHGCU+iTiUE/8b9l8Ftts+zD/1J2L7YL30Hy86 } 10, 50 -- i/GIvK2n8nCEzbSPpDjrcLZwYTkqAcDGWR86TqTK/cYR9QzLm20G22wLB5qWN19R6rAz } 10, 50 -- bz+A== } 10, 50 -- X-Gm-Message-State: AIVw111Dkurehf1z5o9nzzT/1Bt1sufEq/kxjlJDonwCV0g/j8Pb8w1U } 10, 50 -- nNsNqK62+fKLZKZR } 10, 50 -- X-Received: by 10.36.19.138 with SMTP id 132mr13040856itz.65.1502450937060; } 10, 50 -- Fri, 11 Aug 2017 04:28:57 -0700 (PDT) } 10, 50 -- Received: from Nestors-MacBookAir-Pro-2014-ElCapitan-OSX-1011.local ([12.226.178.249]) } 10, 50 -- by smtp.googlemail.com with ESMTPSA id 63sm259530ith.4.2017.08.11.04.28.54 } 10, 50 -- for {microscopy-at-microscopy.com} } 10, 50 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 10, 50 -- Fri, 11 Aug 2017 04:28:56 -0700 (PDT) } 10, 50 -- Subject: viaWWW:TEM cross-sectional glue } 10, 50 -- References: {201708081111.v78BBHLk029596-at-microscopy.com} } 10, 50 -- To: microscopy-at-microscopy.com } 10, 50 -- From: Microscopy Listserver {microscopy.listserver-at-gmail.com} } 10, 50 -- X-Forwarded-Message-Id: {201708081111.v78BBHLk029596-at-microscopy.com} } 10, 50 -- Message-ID: {8497280d-98f1-c78e-cbb6-020b4a18a6c2-at-gmail.com} } 10, 50 -- Date: Fri, 11 Aug 2017 06:28:53 -0500 } 10, 50 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:45.0) } 10, 50 -- Gecko/20100101 Thunderbird/45.8.0 } 10, 50 -- MIME-Version: 1.0 } 10, 50 -- In-Reply-To: {201708081111.v78BBHLk029596-at-microscopy.com} } 10, 50 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 10, 50 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers==============================
X-from: Ribardire Michel {m.ribardiere-at-jeol.fr}
Th main thing is to adjust gun tilt to get Good beam image mission With filament at 100kv must be around 20uA add to 66uA of HT
Filament knob around 9 o'clock With bias adjusted around 5 to 7 Adjust gun tilt x and y to get max brightness
Regards Michel
Envoy depuis mon Sony Xperia T3 d'Orange
---- microscopy.listserver-at-gmail.com a crit ----
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
X-from: mj_iqbal-at-yahoo.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mj_iqbal-at-yahoo as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: mj_iqbal-at-yahoo Name: M Javed Iqbal
Organization: NIBGE Title-Subject: [Filtered] Electron Beam Brightness issue Jeol JEM-1010 after filament change, suggestions required
Message: Hello Dear all suggestions/ help is required regarding the electron microscope filament beam which is not sharp/bright enough (shadow of the fixed aperture) after I replaced the fused filament with new one, in past we have done this practice multiple times without any issue. I have done all the possible filament distance adjusting options with the wehnelt cap/cover but no major difference in results more over obtaining filament saturation point did not yield a converged single very sharp point and when we De-saturate the filament by turning the filament knob counter clockwise its not producing the typical image of two balanced half crescents holing a round spot inside.without engaging any lens aperture the shadow of fixed aperture in having a hallow zone like double spot with slight missing the full overlap Is it possible to post an image or video clip of the same for better understanding ?
Muhammad javed Iqbal TEM Core Facilities Lab NIBGE Pakistan
Login Host: 202.59.89.36 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
I am now retired from ExxonMobil Chemical Company after working 33 in a polymer microscopy lab. I embedded and sectioned samples for TEM/STEM over the last 25 years.
I found that EpoFix, from Ted Pella did a great job for room temperature and heat curing (https://www.emsdiasum.com/microscopy/products/embedding/kits.aspx)
My notes on the use of EpoFix are given below in italics: The issues regarding phase separation of the epoxy-curative mixtures early during the curing process are very real and, if ignored, generally caused significant problems with curing at room temperature. The solution that I developed worked every time when applied correctly.
Polymer samples (films, fabrics, fibers, etc.) are embedded at room temperature or 50C in EpoFix epoxy resin with accelerator (25:3 wt/wt resin:accelerator) according to the manufacturer's directions. (EpoFix is available from Electron Microscopy Sciences although Ted Pella and others may sell a similar resin.)
It is very important to note that the resin-accelerator mixture cures in about 90 minutes but the two phases separate upon standing during the initial 45 minutes or so of curing. To prevent this, the mixture must be manually stirred using a stirring rod, wooden splint, or equivalent for at least two minutes (timed) and continued stirring using a magnetic stir bar/stir plate until the resin-accelerator mixture is stable after 45 to 60 minutes curing; about 60 to 90 minutes, the viscosity of the curing resin gradually increases beyond a usable point.
The resin-accelerator mixture may be cured at 50C for several hours or at room temperature overnight. Blocks cured at ambient temperature may be a little soft after curing overnight; an additional day or more of curing improves cutting performance. Heat curing is preferred if the sample can tolerate 50C temperature without annealing or melting. For example, high density polyethylenes and polypropylenes (melting temperatures ~130C and 155-160C, respectively) were cured at 50C but softer; lower density polyethylenes and elastomers containing low melting polymer fractions that melt or reorganize at or just above room temperature may change the morphology even at these curing temperatures.
For use as a glue, I would cure the EpoFix at room temperature until it is the viscosity you want for a glue.
I hope this helps. I found it to work under all types of polymer samples.
Feel free to contact me with any questions or comments.
Cheers,
Gary M Brown Polymer Microscopist
On Fri, Aug 11, 2017 at 6:55 AM, {microscopy.listserver-at-gmail.com} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: annett.thogersen-at-sintef.no } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both annett.thogersen-at-sintef.no as well as the } Microscopy Listserver } --------------------------------------------------------------------------- } } Email: annett.thogersen-at-sintef.no Name: Annett Thøgersen } } Organization: SINTEF } } Title-Subject: [Filtered] TEM cross-sectional glue } } Message: Dear Colleagues, } } I am looking for a TEM cross-sectional glue that do not need any heat } for hardening, is easy to mix, and will have minimal contamination in } the TEM. I have one that I use now, but unfortunately it softens in } acetone. Does anyone have some good ideas on what glue I can use? I } cannot expose my samples to very much heat. } Thank you, } Annett Thøgersen } } Login Host: 78.91.103.180 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 10, 50 -- From microscopy.listserver-at-gmail.com Fri Aug 11 06:27:21 2017 } 10, 50 -- Received: from mail-it0-f44.google.com (mail-it0-f44.google.com [209.85.214.44]) } 10, 50 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v7BBRLmq000450 } 10, 50 -- for {microscopy-at-microscopy.com} ; Fri, 11 Aug 2017 06:27:21 -0500 } 10, 50 -- Received: by mail-it0-f44.google.com with SMTP id 76so25760458ith.0 } 10, 50 -- for {microscopy-at-microscopy.com} ; Fri, 11 Aug 2017 04:28:57 -0700 (PDT) } 10, 50 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 10, 50 -- d=gmail.com; s=20161025; } 10, 50 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 10, 50 -- :in-reply-to:content-transfer-encoding; } 10, 50 -- bh=l7YWH6wy+lp7x5P7btPs8KynWsXUUHyLl9Ztba8UZ44=; } 10, 50 -- b=abcP64/KXD6U48G9L1KU+FZNDNf049Nyec0YKvCrWyCCMcG7XXIG1CQoAUSWX+vKqt } 10, 50 -- 45UcmjyRUpNdFaaxkdjsIBc/2B/n1roo6uPp5Q+vkqf34480FWGtklR01cauee52HoyE } 10, 50 -- grDOyZa2cCtb8M4jALvVgbX/HeQy5B/3K8r2EwyHkYsSuUD0OkDf2C30a/DHYgzlA74X } 10, 50 -- 15ri/y453b0D5FE3vzPPWL+fdjOoDdbjDhDy5OvePq/PgrctFt2+QEj3qGJM5DvQEX9x } 10, 50 -- Bc2lBY8S4ZUXBsqrCTMSDyDc0X4kMhyWxPpSmYy4B7TWkggbGZu3xDGKxLFcHzi3ff0s } 10, 50 -- wfCA== } 10, 50 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 10, 50 -- d=1e100.net; s=20161025; } 10, 50 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 10, 50 -- :user-agent:mime-version:in-reply-to:content-transfer-encoding; } 10, 50 -- bh=l7YWH6wy+lp7x5P7btPs8KynWsXUUHyLl9Ztba8UZ44=; } 10, 50 -- b=WiK+4nJPPK5SSYVsDuSwAtyStr3Tq8C5g+R2gVQB+HcmA9IOpROrxszaxo2zW1FFdg } 10, 50 -- ExCEW62QNbLjCHsPCxOb/faCgAODtrT2r85isFno0iybiFodLhOi40uqvGS5h0hvOi6E } 10, 50 -- rdMtyMDCQr+WM6ofxSF1CZHwT+NI39plEmvJwOwfJ4qUPVVHk3jMTU2Jxe81zaLKnBEo } 10, 50 -- /kf7XtgFHUs8Tspg2sZjDUIwq5j6gCTHGCU+iTiUE/8b9l8Ftts+zD/1J2L7YL30Hy86 } 10, 50 -- i/GIvK2n8nCEzbSPpDjrcLZwYTkqAcDGWR86TqTK/cYR9QzLm20G22wLB5qWN19R6rAz } 10, 50 -- bz+A== } 10, 50 -- X-Gm-Message-State: AIVw111Dkurehf1z5o9nzzT/1Bt1sufEq/kxjlJDonwCV0g/j8Pb8w1U } 10, 50 -- nNsNqK62+fKLZKZR } 10, 50 -- X-Received: by 10.36.19.138 with SMTP id 132mr13040856itz.65.1502450937060; } 10, 50 -- Fri, 11 Aug 2017 04:28:57 -0700 (PDT) } 10, 50 -- Received: from Nestors-MacBookAir-Pro-2014-ElCapitan-OSX-1011.local ([12.226.178.249]) } 10, 50 -- by smtp.googlemail.com with ESMTPSA id 63sm259530ith.4.2017.08.11.04.28.54 } 10, 50 -- for {microscopy-at-microscopy.com} } 10, 50 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 10, 50 -- Fri, 11 Aug 2017 04:28:56 -0700 (PDT) } 10, 50 -- Subject: viaWWW:TEM cross-sectional glue } 10, 50 -- References: {201708081111.v78BBHLk029596-at-microscopy.com} } 10, 50 -- To: microscopy-at-microscopy.com } 10, 50 -- From: Microscopy Listserver {microscopy.listserver-at-gmail.com} } 10, 50 -- X-Forwarded-Message-Id: {201708081111.v78BBHLk029596-at-microscopy.com} } 10, 50 -- Message-ID: {8497280d-98f1-c78e-cbb6-020b4a18a6c2-at-gmail.com} } 10, 50 -- Date: Fri, 11 Aug 2017 06:28:53 -0500 } 10, 50 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:45.0) } 10, 50 -- Gecko/20100101 Thunderbird/45.8.0 } 10, 50 -- MIME-Version: 1.0 } 10, 50 -- In-Reply-To: {201708081111.v78BBHLk029596-at-microscopy.com} } 10, 50 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 10, 50 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers==============================
--
"An expert is a person who has made all the mistakes which can be made in a very narrow field.” and "Prediction is very difficult, especially if it's about the future." Niels Bohr
Hi Annett, I have used MBond 610 which has to be stored in a refrigerator and sets without any heat in a few hours. It is pretty resistant to acetone.
Debangshu Mukherjee PhD Candidate MatSE, Penn State
On Aug 11, 2017 07:52, {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote:
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver On-Line {http://www.microscopy.com/MicroscopyListserver On-Line} Help http://www.microscopy.com/MicroscopyListserver/FAQ.html {http://www.microscopy.com/MicroscopyListserver/FAQ.html} ----------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html {http://microscopy.com/MicroscopyListserver/MLFormMail.html} --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both annett.thogersen-at-sintef.no {mailto:annett.thogersen-at-sintef.no} as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] TEM cross-sectional glue
Message: Dear Colleagues,
I am looking for a TEM cross-sectional glue that do not need any heat for hardening, is easy to mix, and will have minimal contamination in the TEM. I have one that I use now, but unfortunately it softens in acetone. Does anyone have some good ideas on what glue I can use? I cannot expose my samples to very much heat. Thank you, Annett Thøgersen
Login Host: 78.91.103.180 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Bilinmadison-at-yahoo.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: Bilinmadison-at-yahoo.com Name: Bil Schneider
Organization: UW Madison
Title-Subject: [Filtered] Chamberscope
Message: Our KE GW 25 Infrared Chamberscope died. Looking at options to replace it. Does anyone have suggestions for acquiring a usable GW 25? It fits onto a Hitachi S3400 SEM, or, has anyone upgraded their Chamberscope and could recommend a reliable replacement?
Thanks, Bil Schneider UW Geosciences
Login Host: 144.92.206.210 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
From "www."-at-galaxy.ocn.ne.jp Fri Aug 11 20:43:52 2017 Return-Path: {"www."-at-galaxy.ocn.ne.jp} Received: from mbkd0206.ocn.ad.jp (mbkd0206.ocn.ad.jp [153.149.233.7]) by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v7C1hpZ4030767 for {microscopylistserverarchive6-at-microscopy.com} ; Fri, 11 Aug 2017 20:43:52 -0500 Received: from mf-smf-ucb005.ocn.ad.jp (mf-smf-ucb005.ocn.ad.jp [153.149.231.4]) by mbkd0206.ocn.ad.jp (Postfix) with ESMTP id 8712783B5B; Sat, 12 Aug 2017 10:42:50 +0900 (JST) Received: from mf-smf-ucb005.ocn.ad.jp (mf-smf-ucb005 [153.149.231.4]) by mf-smf-ucb005.ocn.ad.jp (Postfix) with ESMTP id 6B23D6070A; Sat, 12 Aug 2017 10:42:50 +0900 (JST) Received: from msgw008-01.ocn.ad.jp (msgw008-01.ocn.ad.jp [180.37.203.152]) by mf-smf-ucb005.ocn.ad.jp (Switch-3.3.4/Switch-3.3.4) with ESMTP id v7C1gnT2040780; Sat, 12 Aug 2017 10:42:49 +0900 Received: from User (p1269142-ipngn201003fukuokachu.fukuoka.ocn.ne.jp [153.181.81.142]) by msgw008-01.ocn.ad.jp (Postfix) with SMTP id 455F436E4D2; Sat, 12 Aug 2017 10:42:44 +0900 (JST) Reply-To: {Presidency.offiice-at-presidency.com}
X-from: Steve Chapman {protrain-at-emcourses.com}
Hi I see your problem which you described very clearly. The error that you are making is due to making a standard error when aligning an instrument. When we start learning to operate a TEM we worry about the gun alignment and we often make our own problems. The tendency is to see a spot of light and to work on that spot of light, but if it does not respond correctly, this is a shadow! You need to forget the spot of light that does not behave correctly and find the true beam which will behave correctly; your gun is out of alignment when it displays this shadow.
Use your gun alignment shift to find the beam and then gun alignment tilt to bring up the spot and halo that you are familiar with. I suggest you reset all of your gun alignment controls and start again. Good luck
Steve
Steve Chapman FRMS Retired from Protrain for Consultancy and Training in Electron Microscopy +44 (0)7711 606967 web www.emcourses.com
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: 11 August 2017 19:19 To: protrain-at-emcourses.com
X-from: Ribardire Michel {m.ribardiere-at-jeol.fr}
Th main thing is to adjust gun tilt to get Good beam image mission With filament at 100kv must be around 20uA add to 66uA of HT
Filament knob around 9 o'clock With bias adjusted around 5 to 7 Adjust gun tilt x and y to get max brightness
Regards Michel
Envoy depuis mon Sony Xperia T3 d'Orange
---- microscopy.listserver-at-gmail.com a crit ----
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
X-from: mj_iqbal-at-yahoo.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mj_iqbal-at-yahoo as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: mj_iqbal-at-yahoo Name: M Javed Iqbal
Organization: NIBGE Title-Subject: [Filtered] Electron Beam Brightness issue Jeol JEM-1010 after filament change, suggestions required
Message: Hello Dear all suggestions/ help is required regarding the electron microscope filament beam which is not sharp/bright enough (shadow of the fixed aperture) after I replaced the fused filament with new one, in past we have done this practice multiple times without any issue. I have done all the possible filament distance adjusting options with the wehnelt cap/cover but no major difference in results more over obtaining filament saturation point did not yield a converged single very sharp point and when we De-saturate the filament by turning the filament knob counter clockwise its not producing the typical image of two balanced half crescents holing a round spot inside.without engaging any lens aperture the shadow of fixed aperture in having a hallow zone like double spot with slight missing the full overlap Is it possible to post an image or video clip of the same for better understanding ?
Muhammad javed Iqbal TEM Core Facilities Lab NIBGE Pakistan
Login Host: 202.59.89.36 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
From janiceking866-at-gmail.com Sat Aug 12 16:34:06 2017 Return-Path: {janiceking866-at-gmail.com} Received: from gmail.com (mail.dongnamdc.com [222.97.39.100] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v7CLY3Gc031173 for {microscopylistserverarchive6-at-microscopy.com} ; Sat, 12 Aug 2017 16:34:05 -0500 Message-ID: {eec701d313f8$d168f490$18bbdccd-at-janiceking866} Reply-To: "Perry Fox" {janiceking866-at-gmail.com}
X-from: Michael Nesta {mnesta.ebs-at-gmail.com}
Mike,
In my 17 years with EBS, I've never seen or heard of a situation where the failure of one of our Tungsten FIlaments has had a catasrophic effect on a Customer's SEM or TEM. This is not say it is impossible, but given the hundreds of thousands of FIlaments we have manufactured during my tenure, one would think that if it were truly a problem that users need to be concerned about, we would have heard about it happening once or twice...
Sincerely, Mike Nesta President Energy Beam Sciences, Inc. www.ebsciences.com {http://www.ebsciences.com}
On Thu, Aug 3, 2017 at 9:51 PM, {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote:
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver On-Line {http://www.microscopy.com/MicroscopyListserver On-Line} Help http://www.microscopy.com/MicroscopyListserver/FAQ.html {http://www.microscopy.com/MicroscopyListserver/FAQ.html} ----------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html {http://www.microscopy.com/MLFormMail.html} --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both miketoalson-at-gmail.com {mailto:miketoalson-at-gmail.com} as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: miketoalson-at-gmail.com {mailto:miketoalson-at-gmail.com} Name: Mike Toalson
Organization: NanoImages, LLC
Title-Subject: [Filtered] Question to Users of Tungsten Filament SEM
Message: Please help me formulate a stance on a debate about the effects of an SEM's Tungsten filament breaking when they fail or "burn out".
One vendor is raising the alarm that if a user waits until a tungsten filament breaks or fails prior to replacing it, that they run the risk of experiencing a much bigger cleanup issue and even having "shards" from the filament passing over to the Turbo Pump turbines and causing damage.
I've seen plenty of burned out Tungsten filaments but never experienced these issues. Of course, the Wehnelt or Anode can often need a good cleaning which is why most labs have some Pikal or Wenol paste handy.
Other than the interruption to replace a filament, has anybody with a Thermonic Tungsten Filament SEM ever have a major problem from the filament burning out?
Any thoughts on this matter are much appreciated!!
Mike Toalson NanoImages, LLC
Login Host: 47.208.204.209 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Tiffany.Kimble-at-kraftfoods.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Message: Our laboratory is in the market for a stage with both heat and cryo capabilities for our Leica Axiophot Light Microscope. Does anyone have recommendations for stage or stage system? The system I worked on years ago was a dedicated microscope with a system specifically for that purpose. I don't remember the name of the system. Login Host: 108.254.173.144 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
From johnsonloretta061yilke-at-gmail.com Sun Aug 13 18:26:21 2017 Return-Path: {johnsonloretta061yilke-at-gmail.com} Received: from gmail.com ([112.121.163.10]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v7DNQIo9015574 for {microscopylistserverarchive6-at-microscopy.com} ; Sun, 13 Aug 2017 18:26:20 -0500 Received: from [100.78.224.2] by smtp4.cyberemailings.com with ESMTP; Mon, 14 Aug 2017 03:09:03 +0500 Received: from unknown (202.223.96.39) by smtp4.cyberemailings.com with NNFMP; Mon, 14 Aug 2017 02:51:01 +0500 Received: from relay-x.misswldrs.com ([124.154.154.8]) by qnx.mdrost.com with SMTP; Mon, 14 Aug 2017 02:45:10 +0500 Message-ID: {3C3EADDC.86EC09ED-at-gmail.com}
Dear all.
Is there any agreement on the cause of amplitude contrast when imaging proteins in cryo-TEM?
Is it the second order term in the expansion of a weak phase object or is it due to inelastic scattering or the aperture or something else?
All the best.
Philip
==============================Original Headers============================== 6, 23 -- From Philip.Koeck-at-ki.se Tue Aug 15 05:58:03 2017 6, 23 -- Received: from KIMSX09.user.ki.se (kimsx09.user.ki.se [130.229.20.26]) 6, 23 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v7FAw2iL000331 6, 23 -- for {microscopy-at-microscopy.com} ; Tue, 15 Aug 2017 05:58:03 -0500 6, 23 -- Received: from KIMSX02.user.ki.se ([fe80::9419:2c16:a06b:f058]) by 6, 23 -- KIMSX09.user.ki.se ([fe80::18b4:b3da:89f5:13fe%11]) with mapi id 6, 23 -- 14.03.0248.002; Tue, 15 Aug 2017 12:59:51 +0200 6, 23 -- From: Philip Koeck {Philip.Koeck-at-ki.se} 6, 23 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 6, 23 -- Subject: [TEM] amplitude contrast 6, 23 -- Thread-Topic: [TEM] amplitude contrast 6, 23 -- Thread-Index: AdMVtQkPBKAoOm61QjiWDPwk1wqglQAAHzfg 6, 23 -- Date: Tue, 15 Aug 2017 10:59:50 +0000 6, 23 -- Message-ID: {C8D24D7E19AEE543942A826EE910B1C201930C0E44-at-KIMSX02.user.ki.se} 6, 23 -- Accept-Language: en-GB, sv-SE, en-US 6, 23 -- Content-Language: sv-SE 6, 23 -- X-MS-Has-Attach: 6, 23 -- X-MS-TNEF-Correlator: 6, 23 -- x-originating-ip: [130.237.110.133] 6, 23 -- Content-Type: text/plain; charset="us-ascii" 6, 23 -- MIME-Version: 1.0 6, 23 -- Content-Transfer-Encoding: 8bit 6, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v7FAw2iL000331 ==============================End of - Headers==============================
I’m looking for a good reference the describes the rapid increase in data being generated in the microscopy field. With the advent of direct detection, we’re now generating vast amounts of data that require new approaches to handle them. I’m wondering, is there an article that describes these trends over time?
Thank you!
______________________________________ Steven R. Spurgeon, Ph.D. Postdoctoral Research Associate Physical and Computational Sciences Directorate
Pacific Northwest National Laboratory 902 Battelle Boulevard P.O. Box 999 MSIN:K8-87 Richland, WA 99352
This would indeed be a very timely topic. For scanning probe microscopy, there are papers: Big, Deep, and Smart Data in Scanning Probe Microscopy, ACS Nano, 2016, 10 (10), pp 90689086 and Big data and deep data in scanning and electron microscopies: deriving functionality from multidimensional data setsin Advances for Structural and Chemical imaging, 2015 1:6
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Buchsmith-at-gmail.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Has anyone tried Sure Cut Surfactant?
Message: Our lab is cutting large numbers of serial sections. We are having trouble with debris building up on the diamond knife edge so that we need to stop the microtome and clean it. This is problematic for several reasons. I am wondering if anyone has tried Sure Cut added to the resin to improve sectioning? We are embedding in Embed 812 or Hard Plus resin. Thanks JoAnn Buchanan
Login Host: 63.237.233.8 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
Our facility has received a project inquiry in which the investigator would like to study microtubule disruption in cell cultures after various drug treatments. They are also interested in the co-localization of the microtubules with the Golgi and ER. Does anyone have experience with studying microtubules in plastic sections? What is the ideal fixation protocol to preserve the microtubules? Any advice on the subject is much appreciated!
Thank you! -Nick
Nicholas Conoan Electron Microscopy Specialist Wittson Hall 2014 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7292
The information in this e-mail may be privileged and confidential, intended only for the use of the addressee(s) above. Any unauthorized use or disclosure of this information is prohibited. If you have received this e-mail by mistake, please delete it and immediately contact the sender.
==============================Original Headers============================== 7, 65 -- From nicholas.conoan-at-unmc.edu Thu Aug 17 10:47:00 2017 7, 65 -- Received: from mx0b-0026b901.pphosted.com (mx0b-0026b901.pphosted.com [67.231.152.210]) 7, 65 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v7HFl09F020653 7, 65 -- for {Microscopy-at-microscopy.com} ; Thu, 17 Aug 2017 10:47:00 -0500 7, 65 -- Received: from pps.filterd (m0105563.ppops.net [127.0.0.1]) 7, 65 -- by mx0a-0026b901.pphosted.com (8.16.0.21/8.16.0.21) with SMTP id v7HFllJ5006680 7, 65 -- for {Microscopy-at-microscopy.com} ; Thu, 17 Aug 2017 10:48:59 -0500 7, 65 -- Received: from nam03-co1-obe.outbound.protection.outlook.com (mail-co1nam03lp0017.outbound.protection.outlook.com [216.32.181.17]) 7, 65 -- by mx0a-0026b901.pphosted.com with ESMTP id 2cc6e89cwv-1 7, 65 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES256-SHA384 bits=256 verify=OK) 7, 65 -- for {Microscopy-at-microscopy.com} ; Thu, 17 Aug 2017 10:48:59 -0500 7, 65 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 7, 65 -- d=univnebrmedcntr.onmicrosoft.com; s=selector1-unmc-edu; 7, 65 -- h=From:Date:Subject:Message-ID:Content-Type:MIME-Version; 7, 65 -- bh=H2xMtCj/R0Fchfyx3yjoFCtTkrx3dCHChEUaBxpG3jM=; 7, 65 -- b=K1xeXi3OixnWTNHDzq2M77tQBL7sphObGWMB6wkCQmBxEsnz5ZK9oiUPwpkLSc9IA/oW9DIWq7UBsa6upd3DJz/xy1LrFKL1fbKQSnA9Eq5FV+EeMGvB9PcKGiWmWFmao5z26on1jmxwD/OQmvJQ3nZDahZnyDMnDJ0S8ELFx5A= 7, 65 -- Received: from BLUPR0501MB2113.namprd05.prod.outlook.com (10.164.23.143) by 7, 65 -- BLUPR0501MB2020.namprd05.prod.outlook.com (10.164.23.14) with Microsoft SMTP 7, 65 -- Server (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_256_CBC_SHA384_P256) id 7, 65 -- 15.1.1362.12; Thu, 17 Aug 2017 15:48:56 +0000 7, 65 -- Received: from BLUPR0501MB2113.namprd05.prod.outlook.com ([10.164.23.143]) by 7, 65 -- BLUPR0501MB2113.namprd05.prod.outlook.com ([10.164.23.143]) with mapi id 7, 65 -- 15.01.1362.019; Thu, 17 Aug 2017 15:48:56 +0000 7, 65 -- From: "Conoan, Nicholas H" {nicholas.conoan-at-unmc.edu} 7, 65 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 7, 65 -- Subject: Preservation of microtubules 7, 65 -- Thread-Topic: Preservation of microtubules 7, 65 -- Thread-Index: AdMXb1MmXqTsgq8pTMi3mJm5ZuBtjg== 7, 65 -- Date: Thu, 17 Aug 2017 15:48:56 +0000 7, 65 -- Message-ID: {BLUPR0501MB2113F24084052580337D432684830-at-BLUPR0501MB2113.namprd05.prod.outlook.com} 7, 65 -- Accept-Language: en-US 7, 65 -- Content-Language: en-US 7, 65 -- X-MS-Has-Attach: 7, 65 -- X-MS-TNEF-Correlator: 7, 65 -- x-originating-ip: [192.94.102.4] 7, 65 -- x-ms-publictraffictype: Email 7, 65 -- x-microsoft-exchange-diagnostics: 1;BLUPR0501MB2020;6:taHVriqHAsv6uyuE5cEzGAf16GWUwr2E6sE1at0upf6r0g1dkuyp9A5r3Fp7ssRhmS19CKonAkbXFErX+G5MYDrZBbPTOYT4XfxJZ/hbTxSkEfOExpoduoBfKowTMbht32iD025SspAznp6BNE7VGDt1QrjuPWuT3lphb/vkw6XmDN7bIjTM+j+8TG+2l/UIAj4F7adh68PNPtuXjL6fpnrjiKtcW4v0WvxAj5YX2wTVx26bchmwh9p69jzUw5j9qxui4bd59WRtUZ3Rg3Dk3ug33g/Q1OYYzSYApOwXBCYJ++02xnt7FpWitrcVuTWrxenbbNCWAJph1JJmXHvCrQ==;5:mXm78nmAfn7DRQbhJCgf0R8PS9Eb2+6rrq4Ah4+ZVxOXEpvVHQQT5gfxdQeAiph7IkPvuDtL1Xih2mDfQk+c7IsWNyFFFUPrQyxAzqbbAStzIjWnNx/4/jXakaJO9v30V+qMvm3qxwpdZyRux0vYVA==;24:8/hxKXiM+6nNu+5fc2HkUYOJL8lbS35dsjOzMkg72DlOfJTtnrn8OF9FNAAMcwGO8+CjwUPBEeQzCznSPgDJogL41H2UUbz1sNwJvDQwCyw=;7:ac5ixe7cWiQ8qWOKpCQJj8faE2w0pHKrWUjMmcJFEE5HAaAITMEemCSNIoO5oZWfXsA1kbqaoeE52/c28ReJXqFTMmDLLxQPh9DDeNhNW27B8gTfwPb26dcZ9GEGxgmwTAcF7KgTq87PYaQZqLvWEh/ddNAMJJ3wKzXrjQPyt3wT9xXbvniA9TklDhITIfwVFNylRP1a0aEhgw9i2WWyJ8Nq/jIaDSBDTItgL/Es95I= 7, 65 -- x-ms-office365-filtering-correlation-id: 9b767070-3328-4e8f-e024-08d4e5877827 7, 65 -- x-microsoft-antispam: UriScan:;BCL:0;PCL:0;RULEID:(300000500095)(300135000095)(300000501095)(300135300095)(22001)(300000502095)(300135100095)(2017030254152)(300000503095)(300135400095)(2017052603031)(201703131423075)(201703031133081)(201702281549075)(300000504095)(300135200095)(300000505095)(300135600095)(300000506095)(300135500095);SRVR:BLUPR0501MB2020; 7, 65 -- x-ms-traffictypediagnostic: BLUPR0501MB2020: 7, 65 -- x-exchange-antispam-report-test: UriScan:; 7, 65 -- x-microsoft-antispam-prvs: {BLUPR0501MB20204DA697C63F678F93612C84830-at-BLUPR0501MB2020.namprd05.prod.outlook.com} 7, 65 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:(100000700101)(100105000095)(100000701101)(100105300095)(100000702101)(100105100095)(6040450)(601004)(2401047)(5005006)(8121501046)(100000703101)(100105400095)(93006095)(93001095)(10201501046)(3002001)(6041248)(20161123560025)(20161123558100)(20161123555025)(20161123564025)(201703131423075)(201702281529075)(201702281528075)(201703061421075)(201703061406153)(20161123562025)(6072148)(201708071742011)(100000704101)(100105200095)(100000705101)(100105500095);SRVR:BLUPR0501MB2020;BCL:0;PCL:0;RULEID:(100000800101)(100110000095)(100000801101)(100110300095)(100000802101)(100110100095)(100000803101)(100110400095)(100000804101)(100110200095)(100000805101)(100110500095);SRVR:BLUPR0501MB2020; 7, 65 -- x-forefront-prvs: 0402872DA1 7, 65 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10019020)(6009001)(199003)(53754006)(189002)(110136004)(53936002)(7696004)(50986999)(77096006)(6436002)(6506006)(2351001)(2900100001)(189998001)(54356999)(74316002)(66066001)(75432002)(5640700003)(5660300001)(55016002)(105586002)(99286003)(6916009)(9686003)(7116003)(106356001)(33656002)(68736007)(305945005)(3480700004)(7736002)(478600001)(8936002)(8676002)(81166006)(3280700002)(81156014)(97736004)(86362001)(88552002)(2501003)(3660700001)(2906002)(14454004)(101416001)(3846002)(102836003)(6116002)(25786009)(572794001);DIR:OUT;SFP:1102;SCL:1;SRVR:BLUPR0501MB2020;H:BLUPR0501MB2113.namprd05.prod.outlook.com;FPR:;SPF:None;PTR:InfoNoRecords;A:1;MX:1;LANG:en; 7, 65 -- received-spf: None (protection.outlook.com: unmc.edu does not designate 7, 65 -- permitted sender hosts) 7, 65 -- spamdiagnosticoutput: 1:99 7, 65 -- spamdiagnosticmetadata: NSPM 7, 65 -- Content-Type: text/plain; charset="us-ascii" 7, 65 -- MIME-Version: 1.0 7, 65 -- X-OriginatorOrg: unmc.edu 7, 65 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 17 Aug 2017 15:48:56.2604 7, 65 -- (UTC) 7, 65 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted 7, 65 -- X-MS-Exchange-CrossTenant-id: 84a28940-b464-41c3-ba3b-b4fa6665bc05 7, 65 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: BLUPR0501MB2020 7, 65 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:,, definitions=2017-08-17_08:,, 7, 65 -- signatures=0 7, 65 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 suspectscore=0 7, 65 -- malwarescore=0 phishscore=0 adultscore=0 bulkscore=0 classifier=donotscan 7, 65 -- adjust=0 reason=safe scancount=1 engine=8.0.1-1707230000 7, 65 -- definitions=main-1708170261 7, 65 -- Content-Transfer-Encoding: 8bit 7, 65 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v7HFl09F020653 ==============================End of - Headers==============================
Microtubules are not particularly tricky to preserve, just make sure the GA fixative is *not* cold, as that promotes disassembly. There was a lot of such work done in early 80s, I would rather not give just one name and thus leave others out... So called PHEM buffer (originally from Jan de Mey, I think?..) proved particularly good for cytoskeleton, for light and EM. It does result in rather dense (meaning better preserved) cytoplasm, but that should be less of an issue these days with the good digital cameras. Many folks in those old days would rely on more extractive buffers to improve visibility of the microtubules, sometimes even resorting to a mild osmotic shock.
Feel welcome to respond to me directly for the names and more detail.
Best wishes, Vlad
Vlad Speransky, PhD Life Sciences Product Specialist Ted Pella, Inc. http://www.tedpella.com/ 530-243-2200 ext. 266 Cell 530-768-3953 vlad_speransky-at-tedpella.com
---------------------------------------------------------------------------- ---------------------------------- ---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Hello All:
Our facility has received a project inquiry in which the investigator would like to study microtubule disruption in cell cultures after various drug treatments. They are also interested in the co-localization of the microtubules with the Golgi and ER. Does anyone have experience with studying microtubules in plastic sections? What is the ideal fixation protocol to preserve the microtubules? Any advice on the subject is much appreciated!
Thank you! -Nick
Nicholas Conoan Electron Microscopy Specialist Wittson Hall 2014 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7292
The information in this e-mail may be privileged and confidential, intended only for the use of the addressee(s) above. Any unauthorized use or disclosure of this information is prohibited. If you have received this e-mail by mistake, please delete it and immediately contact the sender.
Dear Fellow Microscopists, How do you index electron diffraction patterns recorded by TEM/SAED? Especially for those likely to be of hexagonal, orthorhombic or tetragonal etc. complex crystal structure? I'm looking for some software to aid pattern indexing, commercial or free as long as they are reliable and easy to use. Any suggestions would be highly appreciated please... Zhou
Dr Z Zhou Research Fellow Loughborough Materials Characterisation Centre Department of Materials Loughborough University Loughborough LE11 3TU UK 01509 223163
==============================Original Headers============================== 4, 79 -- From Z.Zhou-at-lboro.ac.uk Fri Aug 18 04:17:04 2017 4, 79 -- Received: from mta-2.lboro.ac.uk (mta-2.lut.ac.uk [158.125.160.48]) 4, 79 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v7I9H33x004524 4, 79 -- for {Microscopy-at-microscopy.com} ; Fri, 18 Aug 2017 04:17:04 -0500 4, 79 -- Received: from [158.125.160.47] (helo=mta-1.lboro.ac.uk) 4, 79 -- by mta-2.lboro.ac.uk with esmtps (TLSv1.2:ECDHE-RSA-AES256-GCM-SHA384:256) 4, 79 -- (Exim 4.89) 4, 79 -- id 1didPN-0004T0-Te 4, 79 -- for Microscopy-at-microscopy.com; Fri, 18 Aug 2017 10:17:37 +0100 4, 79 -- Received: from [158.125.160.17] (helo=itsex-2.lunet.lboro.ac.uk) 4, 79 -- by mta-1.lboro.ac.uk with esmtps (TLSv1.2:ECDHE-RSA-AES256-SHA384:256) 4, 79 -- (Exim 4.89) 4, 79 -- id 1didPL-00077J-DC 4, 79 -- for Microscopy-at-microscopy.com; Fri, 18 Aug 2017 10:17:35 +0100 4, 79 -- Received: from itsex-2.lunet.lboro.ac.uk 4, 79 -- (2001:630:301:d314:580f:144d:cf5e:c130) by itsex-2.lunet.lboro.ac.uk 4, 79 -- (2001:630:301:d314:580f:144d:cf5e:c130) with Microsoft SMTP Server 4, 79 -- (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_256_CBC_SHA384_P384) id 4, 79 -- 15.1.669.32; Fri, 18 Aug 2017 10:17:35 +0100 4, 79 -- Received: from EUR02-AM5-obe.outbound.protection.outlook.com (213.199.180.146) 4, 79 -- by itsex-2.lunet.lboro.ac.uk (158.125.160.17) with Microsoft SMTP Server 4, 79 -- (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_256_CBC_SHA384_P384) id 4, 79 -- 15.1.669.32 via Frontend Transport; Fri, 18 Aug 2017 10:17:35 +0100 4, 79 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=lunet.onmicrosoft.com; 4, 79 -- s=selector1-lboro-ac-uk; 4, 79 -- h=From:Date:Subject:Message-ID:Content-Type:MIME-Version; 4, 79 -- bh=INmiBzyU4DXdy8AZUWC/bLI3qkU6ji3TUqt7IumQmI0=; 4, 79 -- b=WCVIoSuWPtf97kNZYulwiQMa+Ilw1G62i5/jQ6ql/3GjYS6ya4+1nG37df5jAuMjPo749hytEAhrb4yoqBPo26IhrosTelltSDn5cxIgK3Vq1GPCxAkprPn8VhIPjxPAPOPCv1V9WhdasjZy5vpEZzZwETCY1FMYZOZPTrQ9SBc= 4, 79 -- Received: from AM5PR0402MB2771.eurprd04.prod.outlook.com (10.175.41.13) by 4, 79 -- AM5PR0402MB2818.eurprd04.prod.outlook.com (10.175.41.137) with Microsoft SMTP 4, 79 -- Server (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_256_CBC_SHA384_P256) id 4, 79 -- 15.1.1362.18; Fri, 18 Aug 2017 09:17:34 +0000 4, 79 -- Received: from AM5PR0402MB2771.eurprd04.prod.outlook.com 4, 79 -- ([fe80::6d61:7809:2554:a36d]) by AM5PR0402MB2771.eurprd04.prod.outlook.com 4, 79 -- ([fe80::6d61:7809:2554:a36d%14]) with mapi id 15.01.1362.019; Fri, 18 Aug 4, 79 -- 2017 09:17:34 +0000 4, 79 -- From: Zhaoxia Zhou {Z.Zhou-at-lboro.ac.uk} 4, 79 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 4, 79 -- Subject: TEM: softwares to aid selected area diffraction patterns indexing 4, 79 -- Thread-Topic: TEM: softwares to aid selected area diffraction patterns 4, 79 -- indexing 4, 79 -- Thread-Index: AdMYAp0O9n76atmCSimcCjkfTvRjXA== 4, 79 -- Date: Fri, 18 Aug 2017 09:17:33 +0000 4, 79 -- Message-ID: {AM5PR0402MB277141619E829AA6ED4F68E6C6800-at-AM5PR0402MB2771.eurprd04.prod.outlook.com} 4, 79 -- Accept-Language: en-GB, en-US 4, 79 -- Content-Language: en-US 4, 79 -- X-MS-Has-Attach: 4, 79 -- X-MS-TNEF-Correlator: 4, 79 -- authentication-results: spf=none (sender IP is ) 4, 79 -- smtp.mailfrom=Z.Zhou-at-lboro.ac.uk; 4, 79 -- x-originating-ip: [2001:630:301:3064:30ed:151e:2c0f:b363] 4, 79 -- x-ms-publictraffictype: Email 4, 79 -- x-microsoft-exchange-diagnostics: 1;AM5PR0402MB2818;6:cIrG2+7LuFYf5glgB7Brb0/nyar8iITPKsoruCNpz8q+3kgaLV0z9305ZUZ/vmlZUqZsUAL7J5M9azk4JvtI93/hkhirBjvC1mc5JfPL/0ni6dTsLWYJ0g5yqNL/EEICsy5qn/NoPxn//qfKZiSBiKKt9BCYRs+sHsrv39rqIE2DnjHqDBJRPsDsPzgr1V0N8TNVR3JHNbv56z8nRIASlq4sCKdHI2eExBUZxkdneC/l3KnHKKW7PKfn119OMHRUP4DL7AUuQMnJvX8dY9gBqNqzxDzJUjhf1X8/CQMEA9clDiq3hJRj3K24wY6ohDacbPyxKwevICjDrqL9LgDOgQ==;5:mjBwToSnO4+wstv2LJ8c1Ya7KlB/39Wlb5L4Md75fRuQsYuqiQF1LpK9A9OfsG0B4DCl0drghO1O7H6wPlKwL9pGJZZG1FaqpOgc8MycCb8k/jM5Lr1N8EEhALQogeWnz6XTbPIH7MTw8KR3H8PKnw==;24:hl952HHCfAlH4QyQujfyViMHbziqybTouwj9IFgoGsNptJtXceQjOReSZN0Mt/LD5SFhdoj9DFZzmYcRd+j2B2VUFpMm5TTZCbJ0D2rsLK0=;7:Psw7vp4IlqFXEVjIXcmikGfPvvBbEM5O3wZj1SZPK6z4ODsfuca7AnhSOHZLCyRY7nmz29KtKBJ8V1NVqnPC/EjLeGwyv5g3jVMybG38j2IQOD+ZtZ7K9GOwSK3+iAFOz9SbVVC+jthEG3jaiIAIcDwpt0rRDDBm2YaLENfy+mB0ih8aH9/5cMDj/Di8sbt11NwbrYwx9romInSQ81DCEuIYU2f3nFtd32ntvZe8lsM= 4, 79 -- x-ms-exchange-antispam-srfa-diagnostics: SSOS; 4, 79 -- x-ms-office365-filtering-correlation-id: d1beb9a8-53ad-423b-137d-08d4e619f60a 4, 79 -- x-microsoft-antispam: UriScan:;BCL:0;PCL:0;RULEID:(300000500095)(300135000095)(300000501095)(300135300095)(22001)(300000502095)(300135100095)(2017030254152)(300000503095)(300135400095)(2017052603031)(201703131423075)(201703031133081)(201702281549075)(300000504095)(300135200095)(300000505095)(300135600095)(300000506095)(300135500095);SRVR:AM5PR0402MB2818; 4, 79 -- x-ms-traffictypediagnostic: AM5PR0402MB2818: 4, 79 -- x-exchange-antispam-report-test: UriScan:; 4, 79 -- x-microsoft-antispam-prvs: {AM5PR0402MB281896214DB28C747906426AC6800-at-AM5PR0402MB2818.eurprd04.prod.outlook.com} 4, 79 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:(100000700101)(100105000095)(100000701101)(100105300095)(100000702101)(100105100095)(6040450)(601004)(2401047)(8121501046)(5005006)(10201501046)(100000703101)(100105400095)(93006095)(93001095)(3002001)(6041248)(20161123560025)(201703131423075)(201702281529075)(201702281528075)(201703061421075)(201703061406153)(20161123564025)(20161123562025)(20161123555025)(20161123558100)(6072148)(201708071742011)(100000704101)(100105200095)(100000705101)(100105500095);SRVR:AM5PR0402MB2818;BCL:0;PCL:0;RULEID:(100000800101)(100110000095)(100000801101)(100110300095)(100000802101)(100110100095)(100000803101)(100110400095)(100000804101)(100110200095)(100000805101)(100110500095);SRVR:AM5PR0402MB2818; 4, 79 -- x-forefront-prvs: 040359335D 4, 79 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10019020)(6009001)(199003)(189002)(25786009)(101416001)(2906002)(99286003)(8936002)(2900100001)(81156014)(54356999)(7736002)(14454004)(50986999)(8676002)(9686003)(81166006)(3280700002)(33656002)(106356001)(6506006)(74316002)(5660300001)(305945005)(189998001)(6116002)(102836003)(74482002)(97736004)(413944005)(105586002)(72206003)(478600001)(3660700001)(6436002)(68736007)(7696004)(110136004)(5250100002)(42882006)(55016002)(6916009)(53936002)(86362001)(491001);DIR:OUT;SFP:1102;SCL:1;SRVR:AM5PR0402MB2818;H:AM5PR0402MB2771.eurprd04.prod.outlook.com;FPR:;SPF:None;PTR:InfoNoRecords;A:1;MX:1;LANG:en; 4, 79 -- received-spf: None (protection.outlook.com: lboro.ac.uk does not designate 4, 79 -- permitted sender hosts) 4, 79 -- spamdiagnosticoutput: 1:99 4, 79 -- spamdiagnosticmetadata: NSPM 4, 79 -- Content-Type: text/plain; charset="utf-8" 4, 79 -- MIME-Version: 1.0 4, 79 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 18 Aug 2017 09:17:34.1258 4, 79 -- (UTC) 4, 79 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted 4, 79 -- X-MS-Exchange-CrossTenant-id: cf264fc0-aeb8-449f-9054-82ce4454084b 4, 79 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: AM5PR0402MB2818 4, 79 -- X-OriginatorOrg: lboro.ac.uk 4, 79 -- X-Scan-Signature: d0da4f47a0cd8b32bfcc0216a12a6f6e 4, 79 -- X-Lboro-Creds: scanned on mta-1.lboro.ac.uk 4, 79 -- X-Lboro-Filtered: mta-1.lboro.ac.uk, Fri, 18 Aug 2017 10:17:35 +0100 4, 79 -- Content-Transfer-Encoding: 8bit 4, 79 -- X-MIME-Autoconverted: from base64 to 8bit by microscopy.com id v7I9H33x004524 ==============================End of - Headers==============================
Dr Z Zhou asked: How do you index electron diffraction patterns recorded by TEM/SAED?
You may consider JEMS from P. Stadelmann. Have a look to http://www.jems-saas.ch/ that also contain the access to the limited "Student version" for demo on Mac, PC and Linux.
This software is a comprehensive program for (HR) TEM+STEM image simulation and electron diffraction (SAED, nano-diff, CBED, Kikuchi, precession, powder patterns) interpretation and simulation based on Bloch waves and multislice approaches.
In the present case, feeding JEMS with files containing the crystal parameters for all suspected phases allows an automatic match with the experimental patterns for phase identification.
Disclaimer: P. Stadelmann and myself were working in the same laboratory for long though on different subjects.
P. Buffat
==============================Original Headers============================== 7, 22 -- From philippe.buffat-at-epfl.ch Fri Aug 18 07:55:59 2017 7, 22 -- Received: from smtp5.epfl.ch (smtp5.epfl.ch [128.178.224.8]) 7, 22 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v7ICtwvg001840 7, 22 -- for {microscopy-at-microscopy.com} ; Fri, 18 Aug 2017 07:55:58 -0500 7, 22 -- Received: (qmail 30134 invoked by uid 107); 18 Aug 2017 12:57:59 -0000 7, 22 -- X-Virus-Scanned: ClamAV 7, 22 -- Received: from 133.93.198.178.dynamic.wline.res.cust.swisscom.ch (HELO cimepc22.home) (178.198.93.133) (TLS, ECDHE-RSA-AES256-GCM-SHA384 (X25519 curve) cipher) (authenticated) 7, 22 -- by mail.epfl.ch (AngelmatoPhylax SMTP proxy) with ESMTPSA; Fri, 18 Aug 2017 14:57:59 +0200 7, 22 -- X-EPFL-Auth: uGuFJeSWI5fynBn8zaffRvlpYA/tffNLfQSLtsxFCKIq5Kjxfnw= 7, 22 -- Reply-To: philippe.buffat-at-epfl.ch 7, 22 -- To: Microscopy-at-microscopy.com 7, 22 -- From: Philippe Buffat {philippe.buffat-at-epfl.ch} 7, 22 -- Organization: EPFL 7, 22 -- Subject: TEM: softwares to aid selected area diffraction patterns indexing 7, 22 -- Message-ID: {4b078e8c-7ca4-543c-ec88-f5ac88a41de2-at-epfl.ch} 7, 22 -- Date: Fri, 18 Aug 2017 14:57:58 +0200 7, 22 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.10; rv:52.0) 7, 22 -- Gecko/20100101 Thunderbird/52.2.1 7, 22 -- MIME-Version: 1.0 7, 22 -- Content-Type: text/plain; charset=utf-8; format=flowed 7, 22 -- Content-Transfer-Encoding: 7bit 7, 22 -- Content-Language: en-US ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ilchat.sabirov-at-imdea.org as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: ilchat.sabirov-at-imdea.org Name: Ilchat
Organization: IMDEA Materials Institute
Title-Subject: [Filtered] used TEM holder
Message: Dear Colleagues,
We would like to buy a used low background double tilt TEM holder compatible with TALOS. We were searching with Google, but we could not find so far on sale such a holder. Could you recommend any web-site or any company which can potentially sale such equipment? Thank you in advance!
With best regards, Ilchat
Login Host: 88.87.132.251 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both brazda-at-fzu.cz as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: brazda-at-fzu.cz Name: Petr Brazda
Organization: Institute of Physics of the Czech Academy of Science (Prague)
Title-Subject: [Filtered] Dewar for cold trap (CM 120)
Message: Hello,
We just broke our Dewar for the cold trap on our CM 120. Anyone has a spare one to sell?
Best regards,
Petr
Login Host: 147.231.126.120 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
From jeverett397-at-gmail.com Fri Aug 18 21:31:42 2017 Return-Path: {jeverett397-at-gmail.com} Received: from gmail.com (mail.dongnamdc.com [222.97.39.100] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v7J2VdEB005308 for {microscopylistserverarchive6-at-microscopy.com} ; Fri, 18 Aug 2017 21:31:41 -0500 Message-ID: {6c5501d31834$b81b01a0$dacecdb5-at-jeverett397} Reply-To: "Perry Fox" {jeverett397-at-gmail.com}
X-from: Bargar, Tom W {tbargar-at-unmc.edu}
Dear Listers,
Does anyone out there know how the TEM grids we all use are made? I've always been curious, but have not been able to find any information.
Tom Bargar UNMC Electron Microscopy Core Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
The information in this e-mail may be privileged and confidential, intended only for the use of the addressee(s) above. Any unauthorized use or disclosure of this information is prohibited. If you have received this e-mail by mistake, please delete it and immediately contact the sender.
==============================Original Headers============================== 6, 50 -- From microscopy.listserver-at-gmail.com Sat Aug 19 06:05:43 2017 6, 50 -- Received: from mail-io0-f171.google.com (mail-io0-f171.google.com [209.85.223.171]) 6, 50 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v7JB5hKs020959 6, 50 -- for {microscopy-at-microscopy.com} ; Sat, 19 Aug 2017 06:05:43 -0500 6, 50 -- Received: by mail-io0-f171.google.com with SMTP id g35so40067565ioi.3 6, 50 -- for {microscopy-at-microscopy.com} ; Sat, 19 Aug 2017 04:07:49 -0700 (PDT) 6, 50 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 50 -- d=gmail.com; s=20161025; 6, 50 -- h=subject:references:to:from:message-id:date:user-agent:mime-version 6, 50 -- :in-reply-to:content-transfer-encoding; 6, 50 -- bh=xdPyhSuucj0P/gyKPnMX6Sv5gn+Nw4j7cHBPpt5AbGc=; 6, 50 -- b=W6y9OCv7pLZkkj0nU9JjDpqZAEFQNrNaMoSXNDqme4CCsrt808CGCBkg37sPIkYivj 6, 50 -- ySUa7JG0gTRKAIySfV4uZLrPJ8JQfupUvHFZOO2Sq99lUhumQQR5j3ZhwZPRGIshFKdh 6, 50 -- ZreUxOzsjNOxt86Y1Xq5w43hOc7emIcz2M2v9ify/frUFybqn5rHyfriflDInx2W/xAW 6, 50 -- KprOnHPwY0D4Rl+qhtTupe1ujX5iXtY7p4HOO8+kNsP8nz5gDf2bsl39RdRMI9w5eQux 6, 50 -- rLj7Cydb1TLncNF5fnkqT21GZAcn9cjzD3zcid3ENhBl0Vyshg6jtS/VJTqmf42SQmOd 6, 50 -- gAvA== 6, 50 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 50 -- d=1e100.net; s=20161025; 6, 50 -- h=x-gm-message-state:subject:references:to:from:message-id:date 6, 50 -- :user-agent:mime-version:in-reply-to:content-transfer-encoding; 6, 50 -- bh=xdPyhSuucj0P/gyKPnMX6Sv5gn+Nw4j7cHBPpt5AbGc=; 6, 50 -- b=rbq1SioK4KV4ys2wvdI/HJBnlaQXLIIOWh0mR/DX0bUWLdgpegWk1ByDYWksAQvmwM 6, 50 -- vUWhor3JmAQgFHQdBjQROy8Sjl9DmJtFEell3smf0Qnq+aaFikBVKKEmsIC7LmjvY+qP 6, 50 -- lgQox29SK/cSkMKM7aG5FRPCIhQqLoMomVXlGl3+5caPZAYWjGz7p1bsyvtacuF9yaE9 6, 50 -- soTEs+ioNkbFvoF2SWwD1Ogur6QCl/tJje+SE9nEWZyk6PUpGZHR3DgX6r7KdoTYGWax 6, 50 -- 0ebImelFME7ejD/xJHe5W9xicHROVuvEiZ26TPQxuRUclNHWqF7kQriOalFXkW4FYxlh 6, 50 -- Uwaw== 6, 50 -- X-Gm-Message-State: AHYfb5jqcMgjYbqbmlqsELm2NkNwNkSsGLY5YNyfwgdcp1BYiKh7skmL 6, 50 -- S9FAOey3p1mVG16WviA= 6, 50 -- X-Received: by 10.107.184.4 with SMTP id i4mr2312208iof.30.1503140868914; 6, 50 -- Sat, 19 Aug 2017 04:07:48 -0700 (PDT) 6, 50 -- Received: from NestorsnOSX1011.hsd1.il.comcast.net ([2601:248:4201:9d99:2c96:44cd:ec21:4a5f]) 6, 50 -- by smtp.googlemail.com with ESMTPSA id k18sm1690316itb.40.2017.08.19.04.07.47 6, 50 -- for {microscopy-at-microscopy.com} 6, 50 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 6, 50 -- Sat, 19 Aug 2017 04:07:48 -0700 (PDT) 6, 50 -- Subject: Fwd: How are grids made 6, 50 -- References: {BY1PR0501MB131894A52177DE77A31E4D91C4800-at-BY1PR0501MB1318.namprd05.prod.outlook.com} 6, 50 -- To: microscopy-at-microscopy.com 6, 50 -- From: Microscopy Listserver {microscopy.listserver-at-gmail.com} 6, 50 -- X-Forwarded-Message-Id: {BY1PR0501MB131894A52177DE77A31E4D91C4800-at-BY1PR0501MB1318.namprd05.prod.outlook.com} 6, 50 -- Message-ID: {8f5b180c-ddf8-57c0-b542-693f7bb474d1-at-gmail.com} 6, 50 -- Date: Sat, 19 Aug 2017 06:08:04 -0500 6, 50 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:45.0) 6, 50 -- Gecko/20100101 Thunderbird/45.8.0 6, 50 -- MIME-Version: 1.0 6, 50 -- In-Reply-To: {BY1PR0501MB131894A52177DE77A31E4D91C4800-at-BY1PR0501MB1318.namprd05.prod.outlook.com} 6, 50 -- Content-Type: text/plain; charset=windows-1252; format=flowed 6, 50 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I wouldn't know how were made grids you buy from distributors as everyone keeps this "highly confidential," but I've had custom grids produced by process called "Photochemical Etching" or "Photochemical machining:"
It is either one-sided process which can only cut the parts loose, or a two-sided process with exposure and etching happening from both sides of the metal sheet. With two-sided process recesses are easily produced by exposing one side only and etching 50% of the metal sheet thickness. There are plenty of OEMs in US, Europe, and China, who would happily produce custom grids to your design within the limitations of available to them process, if you could fork up one-time tooling charge (usually couple hundred dollars) and put up with minimal order requirement which varies between vendors from 1 or 2 sheet sized from 8/5"x11" to 1mx1m.
Stay curious :) Valery
Valery Ray ============================== PBS&T, MEO Engineering Company 290 Broadway, Suite 298 Methuen, MA 01844, USA Phone: +1-978-305-0479 - leave a message Mobie: +1-978-305-0479 - leave a message E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
-------------------------------------------- On Sat, 8/19/17, {microscopy.listserver-at-gmail.com} wrote:
Subject: [Microscopy] Fwd: How are grids made To: vray-at-partbeamsystech.com Date: Saturday, August 19, 2017, 7:07 AM
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html ----------------------------------------------------------------------------
X-from: Bargar, Tom W {tbargar-at-unmc.edu}
Dear Listers,
Does anyone out there know how the TEM grids we all use are made? I've always been curious, but have not been able to find any information.
Tom Bargar UNMC Electron Microscopy Core Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
The information in this e-mail may be privileged and confidential, intended only for the use of the addressee(s) above. Any unauthorized use or disclosure of this information is prohibited. If you have received this e-mail by mistake, please delete it and immediately contact the sender.
==============================Original Headers============================== 6, 50 -- From microscopy.listserver-at-gmail.com Sat Aug 19 06:05:43 2017 6, 50 -- Received: from mail-io0-f171.google.com (mail-io0-f171.google.com [209.85.223.171]) 6, 50 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v7JB5hKs020959 6, 50 -- for {microscopy-at-microscopy.com} ; Sat, 19 Aug 2017 06:05:43 -0500 6, 50 -- Received: by mail-io0-f171.google.com with SMTP id g35so40067565ioi.3 6, 50 -- for {microscopy-at-microscopy.com} ; Sat, 19 Aug 2017 04:07:49 -0700 (PDT) 6, 50 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 50 -- d=gmail.com; s=20161025; 6, 50 -- h=subject:references:to:from:message-id:date:user-agent:mime-version 6, 50 -- :in-reply-to:content-transfer-encoding; 6, 50 -- bh=xdPyhSuucj0P/gyKPnMX6Sv5gn+Nw4j7cHBPpt5AbGc=; 6, 50 -- b=W6y9OCv7pLZkkj0nU9JjDpqZAEFQNrNaMoSXNDqme4CCsrt808CGCBkg37sPIkYivj 6, 50 --
X-from: Bill & Sue Tivol {wtivol-at-sbcglobal.net}
Hi Tom, I think some grids, including the standard copper ones, are made by electrodeposition. I am pretty sure that different processes are used just by looking at the edges of the grid bars. Molybdenum grids, for example, have much rougher grid bars than copper or titanium grids. Yours, Bill
==============================Original Headers============================== 5, 50 -- From microscopy.listserver-at-gmail.com Sun Aug 20 16:26:02 2017 5, 50 -- Received: from mail-io0-f182.google.com (mail-io0-f182.google.com [209.85.223.182]) 5, 50 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v7KLQ2oo002343 5, 50 -- for {microscopy-at-microscopy.com} ; Sun, 20 Aug 2017 16:26:02 -0500 5, 50 -- Received: by mail-io0-f182.google.com with SMTP id 1so11794969ioy.2 5, 50 -- for {microscopy-at-microscopy.com} ; Sun, 20 Aug 2017 14:28:13 -0700 (PDT) 5, 50 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 5, 50 -- d=gmail.com; s=20161025; 5, 50 -- h=subject:references:to:from:message-id:date:user-agent:mime-version 5, 50 -- :in-reply-to:content-transfer-encoding; 5, 50 -- bh=pk0hIFrEHGmPVXABTqUF7qVmjvorV3QWkE9yvJBcBYU=; 5, 50 -- b=l5SgWirGLdaPIAX48nY20KUAZIF6dDc3DGG3DF/KvDIIVmn/CP1jee6qxsPGZ4JZ/S 5, 50 -- POscjDt06Odkytnjz1OjDedxg3UnFs9ZjaYIvb1XWhzmKfzT0uaTsiYX4rWnmTK9o0jW 5, 50 -- xh4ALyPFS6xqn7hkf094vv0wSUe7hfqFTp3adUwmYUCZfeOYcMlAPyvRMs0cA7YwQn3A 5, 50 -- 7AJ4VHm3N+w/FK+b8hLLgkEogAgzmV+FZgCNtGtSM9O/bIdy+cWBx8eKAGMkMMBw+mBE 5, 50 -- 2YDZC+aXRI05il7yFinziDUGlvbu5MHBGPXGE26Z1ke1+MI59csgyOPDJieWZMNs/xFd 5, 50 -- 9Oqw== 5, 50 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 5, 50 -- d=1e100.net; s=20161025; 5, 50 -- h=x-gm-message-state:subject:references:to:from:message-id:date 5, 50 -- :user-agent:mime-version:in-reply-to:content-transfer-encoding; 5, 50 -- bh=pk0hIFrEHGmPVXABTqUF7qVmjvorV3QWkE9yvJBcBYU=; 5, 50 -- b=ZXh5z/S6dhwIxuRog1rg/HVjD/iAPA7Pmg+lpgI7VVfkoF1dnLWnnxVHsUBa1ieJdK 5, 50 -- aZMPeXJDmyykLV8AVHw5+sEmn8oZ4RvJR2PaW7K+OJXn04maclOxaqirIfTcZZIUPgBu 5, 50 -- uSPZQM61HaQMxwH3qrfzYb5C/3T4mQZAENu8gAp0EiCN8/OeQEUHw0vX91ESbrKMVvPh 5, 50 -- amNwtYkSt+G2NHKEVVbfCzEMTuW9CtHaB/AA9RJT6QyAlwLh7To/q6f8xyAqavTtOmN+ 5, 50 -- 29tJnKZWq5rnXlapvu9pc/cydP8KNWaTsY3rKYYKdpJWQ7KdmCxIenMqVqwmF4mHircI 5, 50 -- zQKQ== 5, 50 -- X-Gm-Message-State: AHYfb5gu9LJVMOSdevaJsH/CnoTQRDn8tumEX7NZf2GAFK0c4NXqpdOg 5, 50 -- T5lTHW8vGCD5No03 5, 50 -- X-Received: by 10.107.131.10 with SMTP id f10mr7092411iod.141.1503264493068; 5, 50 -- Sun, 20 Aug 2017 14:28:13 -0700 (PDT) 5, 50 -- Received: from eduroam062-077.wl.anl-external.org (eduroam062-077.wl.anl-external.org. [130.202.62.77]) 5, 50 -- by smtp.googlemail.com with ESMTPSA id g200sm1148754itb.3.2017.08.20.14.28.11 5, 50 -- for {microscopy-at-microscopy.com} 5, 50 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 5, 50 -- Sun, 20 Aug 2017 14:28:12 -0700 (PDT) 5, 50 -- Subject: Re: [Microscopy] Fwd: How are grids made 5, 50 -- References: {83461A76-FFE2-4445-9B28-5297932C5D2A-at-sbcglobal.net} 5, 50 -- To: microscopy-at-microscopy.com 5, 50 -- From: Microscopy Listserver {microscopy.listserver-at-gmail.com} 5, 50 -- X-Forwarded-Message-Id: {83461A76-FFE2-4445-9B28-5297932C5D2A-at-sbcglobal.net} 5, 50 -- Message-ID: {9708d4d6-544d-f315-2d94-27b43228f64e-at-gmail.com} 5, 50 -- Date: Sun, 20 Aug 2017 16:28:35 -0500 5, 50 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:45.0) 5, 50 -- Gecko/20100101 Thunderbird/45.8.0 5, 50 -- MIME-Version: 1.0 5, 50 -- In-Reply-To: {83461A76-FFE2-4445-9B28-5297932C5D2A-at-sbcglobal.net} 5, 50 -- Content-Type: text/plain; charset=windows-1252; format=flowed 5, 50 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Hello, I've just replied to Petr in Czech off-line.
Here is a summary: The producer of Dewar for cold trap used in Philips CM microscopes is also offering Glass Refills: {http://www.kgw-isotherm.com/produkte/dewar/00.html}
Best regards
Oldrich
-- Oldřich Benada Institute of Microbiology CAS, v.v.i. Laboratory of Molecular Structure Characterization Vídeňská 1083 142 20 Prague 4 Czech Republic
On Fri, 18 Aug 2017 20:03:11 -0500, microscopy.listserver-at-gmail.com wrote : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: brazda-at-fzu.cz } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both brazda-at-fzu.cz as well as the Microscopy } Listserver } --------------------------------------------------------------------------- } } Email: brazda-at-fzu.cz Name: Petr Brazda } } Organization: Institute of Physics of the Czech Academy of Science } (Prague) } } Title-Subject: [Filtered] Dewar for cold trap (CM 120) } } Message: Hello, } } We just broke our Dewar for the cold trap on our CM 120. Anyone has a } spare one to sell? } } Best regards, } } Petr } } Login Host: 147.231.126.120 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original } Headers============================== 12, 52 -- From } microscopy.listserver-at-gmail.com Fri Aug 18 20:00:07 2017 12, 52 -- } Received: from mail-it0-f42.google.com (mail-it0-f42.google.com } [209.85.214.42]) 12, 52 -- by microscopy.com } (8.12.11.20060308/8.12.8) with ESMTP id v7J107xt024457 12, 52 -- } for {microscopy-at-microscopy.com} ; Fri, 18 Aug 2017 20:00:07 } -0500 12, 52 -- Received: by mail-it0-f42.google.com with SMTP id } 77so13772082itj.1 12, 52 -- for {microscopy-at-microscopy.com} ; } Fri, 18 Aug 2017 18:02:11 -0700 (PDT) 12, 52 -- DKIM-Signature: v=1; } a=rsa-sha256; c=relaxed/relaxed; 12, 52 -- d=gmail.com; } s=20161025; 12, 52 -- } h=subject:references:to:from:message-id:date:user-agent:mime-version } 12, 52 } -- :in-reply-to:content-language:content-transfer-encoding; } 12, 52 -- bh=mctyki6adWWjtNkiSQsvl/ao8DLUFeHFDoszUCrbRYI=; } 12, 52 -- } b=nOfsBffOuvnpicYgO8Dh58sycyux8/qVCA2/Ea+xOoPFSBk6IGCmFvE1DEpJ0hw8W0 } 12, 52 -- } v+qtEPjnb6sRyK5eCd/3ZzOWYK8clwA6euch/LfNvpWpvL9ivWWK2Nu9u+OeUsyLQIEP } 12, 52 -- } tBoN0lNC4kyB0tQN2hwI32YhnqleEdF8OXh7zMHpnrAg6vLw5VrDGK7WtseyZpe8ukUr } 12, 52 -- } KOhkfMunzvtHcvjPrNdMiggmpQwuvdFXrbpLiCOtwYDMVe6v9NCRPeDJQCXxsvu2ej82 } 12, 52 -- } 1QTHhZTccadrHhgE796v4KlklCsLP0xLLbZuhsttE97fXjbyPRjOikQA2n4PTo3IyeGR } 12, 52 -- ndJw== 12, 52 -- X-Google-DKIM-Signature: v=1; } a=rsa-sha256; c=relaxed/relaxed; 12, 52 -- d=1e100.net; } s=20161025; 12, 52 -- } h=x-gm-message-state:subject:references:to:from:message-id:date 12, } 52 -- :user-agent:mime-version:in-reply-to:content-language } 12, 52 -- :content-transfer-encoding; 12, 52 -- } bh=mctyki6adWWjtNkiSQsvl/ao8DLUFeHFDoszUCrbRYI=; 12, 52 -- } b=GnzKhKoQEPOQQsY+APrQmgqNHZCVNqX522H6FHMvVTeJCPkcZVJ4XYccHuiwjlNHVl } 12, 52 -- } OsHhcbRTMDd6DJZrtMZ6sUcMWfOTCLx3mccnjDcBjDU0j+dis+/Mh8bo2Ax1aZsAAfMx } 12, 52 -- } ojYX3o7da2jKEEGQmXQtvMIFXAB4qTrNtWZNIlKduPuIgnL6gRzSoeFOhL8m8Bhbjeiq } 12, 52 -- } jwDEn40OiwyjisTGzS2/nX1qgcpkZoNjTJHyPScf4p4dsVaqgRkYkoBdKY52nqtkkJDd } 12, 52 -- } CpUwEVBCxc5glldyXy65q3dXtYfIyKw9gG7pQJpfeJIjdKVrh4STqT/Nb9tzJcpwbiDY } 12, 52 -- cQ5g== 12, 52 -- X-Gm-Message-State: } AHYfb5gRTwOEVr/m6GfeLlWwe8rx8IDK2ci1VPxkYoFHdQA7l8KVTDNC 12, 52 -- } r78pIGnadG2TeR55+xk= 12, 52 -- X-Received: by 10.36.9.75 with
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both f.long-at-queensu.ca as well as the Microscopy Listserver ---------------------------------------------------------------------------
I am looking for a good method in fitting the EELS plasmon peak for identification phases with subtle hydrogen concentration differences. The NLLS gaussian fitting function in the Gatan GMS software seems not able to detect the very small energy shift. If any EELS expert can give suggestions in analyzing the plasmon peak that will be great.
Best regards,
Fei
Login Host: 74.14.231.44 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
Hyperspy has an excellent library of EELS functions that can help you do this in an automated fashion. It’s written in Python, so the syntax isn’t too difficult to learn.
Here’s a link: http://hyperspy.org/hyperspy-doc/current/user_guide/eels.html
______________________________________ Steven R. Spurgeon, Ph.D. Postdoctoral Research Associate Physical and Computational Sciences Directorate
Pacific Northwest National Laboratory 902 Battelle Boulevard P.O. Box 999 MSIN:K8-87 Richland, WA 99352
On 8/21/17, 8:49 AM, "microscopy.listserver-at-gmail.com" {microscopy.listserver-at-gmail.com} wrote:
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html ----------------------------------------------------------------------------
X-from: f.long-at-queensu.ca
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both f.long-at-queensu.ca as well as the Microscopy Listserver ---------------------------------------------------------------------------
I am looking for a good method in fitting the EELS plasmon peak for identification phases with subtle hydrogen concentration differences. The NLLS gaussian fitting function in the Gatan GMS software seems not able to detect the very small energy shift. If any EELS expert can give suggestions in analyzing the plasmon peak that will be great.
Best regards,
Fei
Login Host: 74.14.231.44 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
Hello Our Jeol SEM JSM5600LV doesn't get vacuum ready. Normally pre-evacuation and evacuation phases last 2' each, now pre-evacuation lasts 2' but evacuation phase seems endless After 1h no vacuum ready appeared neither any message from the software. Can be mechanical or electronic failure or both? Any help to tackle the p[problem will be greatly appreciated yorgos
Personally I do not have any experiences with SEM JSM5600LV. However, a long time ago we had a similar problem on Balzer BAF 301 Freeze etching device. The problem was in a pirani gauge. It was heavily contaminated. Therefore the vacuum level readout was completely wrong. In the Balzers manual there was a procedure how to clean the pirani gauge and it was working well. The cleaning of the pirani gauge solved our problem. So, I would suggest to check pirani gauge in your SEM. It might help you.
Best regards
Oldrich
-- Oldřich Benada Institute of Microbiology CAS, v.v.i. Laboratory of Molecular Structure Characterization Vídeňská 1083 142 20 Prague 4 Czech Republic
On Tue, 22 Aug 2017 05:56:25 -0500, eikonika-at-otenet.gr wrote : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello } Our Jeol SEM JSM5600LV doesn't get vacuum ready. Normally } pre-evacuation and evacuation phases last 2' each, now pre-evacuation } lasts 2' but evacuation phase seems endless After 1h no vacuum ready } appeared neither any message from the software. } Can be mechanical or electronic failure or both? } Any help to tackle the p[problem will be greatly appreciated } yorgos } } } Dr Yorgos Nikas } Athens_ Innovative_ Microscopy } Skra 36 Voula 16673 GREECE } _ } Tel/fax +30 210 8957677 } mobile +30 6945 107477 } www.eikonika.net_www.aim.cat } ************************************* } } } } } ==============================Original } Headers============================== 5, 20 -- From } eikonika-at-otenet.gr Tue Aug 22 05:52:42 2017 5, 20 -- Received: from } medusa.otenet.gr (smtp-out31.otenet.gr [83.235.69.31]) 5, 20 -- } by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } v7MAqfvO001680 5, 20 -- for {microscopy-at-microscopy.com} ; Tue, } 22 Aug 2017 05:52:42 -0500 5, 20 -- Received: from ozymandias } (ppp-2-84-164-38.home.otenet.gr [2.84.164.38]) 5, 20 -- by } medusa.otenet.gr (ESMTP) with ESMTPSA 5, 20 -- for } {microscopy-at-microscopy.com} ; Tue, 22 Aug 2017 14:40:13 +0300 (EEST) } 5, 20 -- From: "Yorgos Nikas" {eikonika-at-otenet.gr} 5, 20 -- To: } {microscopy-at-microscopy.com} 5, 20 -- Subject: JSM5600LV evacuation } arrest 5, 20 -- Date: Tue, 22 Aug 2017 14:40:10 +0300 5, 20 -- } Message-ID: {000001d31b3b$69206ef0$3b614cd0$-at-otenet.gr} 5, 20 -- } MIME-Version: 1.0 5, 20 -- Content-Type: text/plain; } 5, 20 -- charset="iso-8859-1" } 5, 20 -- X-Mailer: Microsoft Outlook 14.0 } 5, 20 -- Thread-Index: AdMbOj30K+/dNsvSQ9O6oecqI2b1xw== } 5, 20 -- Content-Language: en-us } 5, 20 -- Content-Transfer-Encoding: 8bit } 5, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } microscopy.com id v7MAqfvO001680 ==============================End of } - Headers==============================
Upozorneni: Neni-li v teto zprave vyslovne uvedeno jinak, ma tato E-mailova zprava nebo jeji prilohy pouze informativni charakter. Tato zprava ani jeji prilohy v zadnem ohledu ustavy AV CR, v.v.i. k nicemu nezavazuji. Text teto zpravy nebo jejich priloh neni navrhem na uzavreni smlouvy, ani prijetim pripadneho navrhu na uzavreni smlouvy, ani jinym pravnim jednanim smerujicim k uzavreni jakekoliv smlouvy a nezaklada predsmluvni odpovednost ustavu AV CR, v.v.i.
Disclaimer: If not expressly stated otherwise, this e-mail message (including any attached files) is intended purely for informational purposes and does not represent a binding agreement on the part of Institutes of CAS. The text of this message and its attachments cannot be considered as a proposal to conclude a contract, neither the acceptance of a proposal to conclude a contract, nor any other legal act leading to concluding any contract; nor does it create any pre-contractual liability on the part of Institutes of CAS.
==============================Original Headers============================== 11, 46 -- From benada-at-biomed.cas.cz Tue Aug 22 06:30:01 2017 11, 46 -- Received: from barracuda.biomed.cas.cz (barracuda.biomed.cas.cz [147.231.40.11]) 11, 46 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v7MBU0pJ024805 11, 46 -- for {microscopy-at-microscopy.com} ; Tue, 22 Aug 2017 06:30:01 -0500 11, 46 -- X-ASG-Debug-ID: 1503405225-05011e15a01c5690001-4CH8be 11, 46 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) by barracuda.biomed.cas.cz with ESMTP id je6AQlBFEWk0V7xV; Tue, 22 Aug 2017 14:33:45 +0200 (CEST) 11, 46 -- X-Barracuda-Envelope-From: benada-at-biomed.cas.cz 11, 46 -- X-Barracuda-Effective-Source-IP: mail2.biomed.cas.cz[147.231.40.32] 11, 46 -- X-Barracuda-Apparent-Source-IP: 147.231.40.32 11, 46 -- Received: from u117ob02 (nb170ph.mbu.cas.cz [147.231.44.133]) 11, 46 -- (using TLSv1.2 with cipher ECDHE-RSA-AES128-GCM-SHA256 (128/128 bits)) 11, 46 -- (No client certificate requested) 11, 46 -- by mail2.biomed.cas.cz (Postfix) with ESMTPSA id 6F7F8D01945; 11, 46 -- Tue, 22 Aug 2017 14:33:41 +0200 (CEST) 11, 46 -- Date: Tue, 22 Aug 2017 14:33:41 +0200 11, 46 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 11, 46 -- To: eikonika-at-otenet.gr 11, 46 -- Cc: {microscopy-at-microscopy.com} 11, 46 -- Subject: Re: [Microscopy] JSM5600LV evacuation arrest 11, 46 -- Message-ID: {20170822143341.32e40d81-at-u117ob02} 11, 46 -- X-ASG-Orig-Subj: Re: [Microscopy] JSM5600LV evacuation arrest 11, 46 -- In-Reply-To: {201708221056.v7MAuP1M004540-at-microscopy.com} 11, 46 -- References: {201708221056.v7MAuP1M004540-at-microscopy.com} 11, 46 -- Organization: =?UTF-8?B?TWlrcm9iaW9sb2dpY2vDvSDDunN0YXY=?= AV 11, 46 -- =?UTF-8?B?xIxS?= 11, 46 -- X-Mailer: Claws Mail 3.14.1 (GTK+ 2.24.25; i586-pc-linux-gnu) 11, 46 -- MIME-Version: 1.0 11, 46 -- Content-Type: text/plain; charset=UTF-8 11, 46 -- X-IoP-CAS-MailScanner-Information: Please contact the ISP for more information 11, 46 -- X-IoP-CAS-MailScanner-ID: 6F7F8D01945.A81FB 11, 46 -- X-IoP-CAS-MailScanner: Processed 11, 46 -- X-Spam-Status: No 11, 46 -- X-Barracuda-Connect: mail2.biomed.cas.cz[147.231.40.32] 11, 46 -- X-Barracuda-Start-Time: 1503405225 11, 46 -- X-Barracuda-URL: https://barracuda.biomed.cas.cz:443/cgi-mod/mark.cgi 11, 46 -- X-Virus-Scanned: by bsmtpd at biomed.cas.cz 11, 46 -- X-Barracuda-Scan-Msg-Size: 4158 11, 46 -- X-Barracuda-BRTS-Status: 1 11, 46 -- X-Barracuda-Spam-Score: 0.00 11, 46 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using per-user scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=7.0 KILL_LEVEL=1000.0 tests= 11, 46 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.42195 11, 46 -- Rule breakdown below 11, 46 -- pts rule name description 11, 46 -- ---- ---------------------- -------------------------------------------------- 11, 46 -- Content-Transfer-Encoding: 8bit 11, 46 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v7MBU0pJ024805 ==============================End of - Headers==============================
Our facility provides EM support to several research projects studying ultrastructural changes of the brain, optic nerve, and other neural tissues using large animals such as dogs and monkeys. We often see various degrees of myelin sheet bubbling and occasional interstitial edema of mitochondria even in control (untreated) samples. The distortions are often inconsistent and the degree can vary from one specimen block to another. We suspect it is a fixation problem as it is difficult to do perfusion fix with large animals and sometimes impossible as the researchers often need to collect samples for biochemical analysis as well. I am just wondering if anyone has some insights or a workaround to minimize the myelin distortion. I would also appreciate any tips on preserving myelin ultrastructure during TEM embedding. Thanks.
Best regards, Ru-ching Hsia Electron Microscopy Core Imaging Facility University of Maryland Baltimore
==============================Original Headers============================== 5, 60 -- From hsia627-at-hotmail.com Tue Aug 22 10:57:15 2017 5, 60 -- Received: from NAM02-CY1-obe.outbound.protection.outlook.com (mail-oln040092004039.outbound.protection.outlook.com [40.92.4.39]) 5, 60 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v7MFvEZI023306 5, 60 -- for {Microscopy-at-microscopy.com} ; Tue, 22 Aug 2017 10:57:14 -0500 5, 60 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=hotmail.com; 5, 60 -- s=selector1; h=From:Date:Subject:Message-ID:Content-Type:MIME-Version; 5, 60 -- bh=iT32yLMiC7mIC7+bdkCGzcP9Ok4eOevi21ybbpOtEHk=; 5, 60 -- b=D3jgwRLQq8SU974c6zdedzJQfcPYPLZ3yN+4O8OlpCi5VfxNTBWof/maWBWrYo54vKqdXV1ofXvni0d9OGj1eBX73sqeZKY+eBvIbVnOPM1lho3q4WFK9yzwBFvX5JrG/+nDQy4V8UTzwWsCS+oUvLk1Q9EV1TNF5LQw9P2TYSpXDmqDNHswy9y3tW0rjmsM0jarFQ9TchpIuCCQ6IXFLUFARUVie7avylpP/0LS0y/DJNtdHszcCrowPQcaaxaLl6iXMeXSeQv1no67iwuVvIOXFZL6QyNeVcTJc/Ax82bmHFNqjuGYFrWTA1NuiPxqJta5yGPLWGy89j/uzhpdYw== 5, 60 -- Received: from CY1NAM02FT013.eop-nam02.prod.protection.outlook.com 5, 60 -- (10.152.74.56) by CY1NAM02HT209.eop-nam02.prod.protection.outlook.com 5, 60 -- (10.152.74.92) with Microsoft SMTP Server (version=TLS1_2, 5, 60 -- cipher=TLS_ECDHE_RSA_WITH_AES_256_CBC_SHA384_P384) id 15.1.1341.15; Tue, 22 5, 60 -- Aug 2017 17:01:02 +0000 5, 60 -- Received: from BN1PR04MB842.namprd04.prod.outlook.com (10.152.74.51) by 5, 60 -- CY1NAM02FT013.mail.protection.outlook.com (10.152.75.162) with Microsoft SMTP 5, 60 -- Server (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_128_CBC_SHA256_P256) id 5, 60 -- 15.1.1341.15 via Frontend Transport; Tue, 22 Aug 2017 17:01:02 +0000 5, 60 -- Received: from BN1PR04MB842.namprd04.prod.outlook.com 5, 60 -- ([fe80::91f:ba59:8471:7719]) by BN1PR04MB842.namprd04.prod.outlook.com 5, 60 -- ([fe80::91f:ba59:8471:7719%18]) with mapi id 15.01.1362.019; Tue, 22 Aug 2017 5, 60 -- 17:01:02 +0000 5, 60 -- From: Ruching hsia {hsia627-at-hotmail.com} 5, 60 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 5, 60 -- Subject: Preservation of myelin ultrastructure in TEM embedding 5, 60 -- Thread-Topic: Preservation of myelin ultrastructure in TEM embedding 5, 60 -- Thread-Index: AQHTG2futSE7jr1b9kyQaX6v/Q/DVA== 5, 60 -- Date: Tue, 22 Aug 2017 17:01:02 +0000 5, 60 -- Message-ID: {BN1PR04MB84282C2D884D398B5DDC230EF840-at-BN1PR04MB842.namprd04.prod.outlook.com} 5, 60 -- Accept-Language: en-US 5, 60 -- Content-Language: en-US 5, 60 -- X-MS-Has-Attach: 5, 60 -- X-MS-TNEF-Correlator: 5, 60 -- authentication-results: microscopy.com; dkim=none (message not signed) 5, 60 -- header.d=none;microscopy.com; dmarc=none action=none header.from=hotmail.com; 5, 60 -- x-incomingtopheadermarker: OriginalChecksum:C5DAEFB6D5352916BD31D2334469C0F091637A71CBA68E13B63B5909CB9801A0;UpperCasedChecksum:2AE08542673A79EA09B072E45E34374443388E32312DC530DC1C3379F92D0C9D;SizeAsReceived:6909;Count:44 5, 60 -- x-ms-exchange-messagesentrepresentingtype: 1 5, 60 -- x-tmn: [niWsbDnQU99s/QXy5dlDyE/XThNkCtUCs1FMz1lWIVo=] 5, 60 -- x-ms-publictraffictype: Email 5, 60 -- x-microsoft-exchange-diagnostics: 1;CY1NAM02HT209;6:2Qi0u5Z6yJczSAkODtfk9gKeX63iSETLcONsTLLzBhSMMbzV7jvImLBQAEsmmTUS15BXjsJfgiuQTTKqnohkilImJczD7pvwIvJSakq0NQxNAS0/jS4dL/PvnKVbZqBD2HORcxEUHKQVQQ7tE6TOBT4ntTr9AwDtV1V2m77mMd6wlyewtVIkvLpWwFLRceQsygTjYCTMFdI8dH/OonYwWFlnqS3GqydJfvv81tqXN0D2qFigyUbjOFwZoETq1U8eRJstJfNpY5GQVCf8V9FesA3nx6qfpVP1Yu7FgtPaIEvRNr27kW2M8qhx6OQeWzvLBYKJj9tA0yM/lW4IHAzN2w==;5:cwUzUheNfGlOOq4vvYOwsTYZLRUld3EcT6IbpG/hIu8DMgJoFpZWCavlXGPAN9l6xxKOpI/iLyjAOSh5ni/uhYbmf/D8bePsRMLCFqWRAl878wh5hGbmnB9tQq5TVx8OP8KyIrAfDCA1rjoJdIE/dg==;24:O8jlxCtkR8Lj6bSBNraxnccmOnBgFW6K/L+4plTRi9cr+lo67torvNaXIGcKlsuqE82eHGMMT2IKt0f5FkICcFijsvYbVrRVl07bEQopHE8=;7:pZZAwc5LxO5kku0GCs04CQ0aLrEhF70aI/iOtHRV5x6ilt30lpJ3KckNgScbNRwY37I7WRi0KS/86SXL+w9d4WDUjfr5jytVVqOiq4equdPGIm+tBm/pXjm/tJFo9n3C7SqW8EFygbbfAu6agt/GcpL992SimM8pJ//tCCs5FI/Kn1eEJ18hpfJsawVNK4KsCyU+sljlOGt49nbWhwNZLKsCogWT5+baOB/cp/+I7NE= 5, 60 -- x-incomingheadercount: 44 5, 60 -- x-eopattributedmessage: 0 5, 60 -- x-forefront-antispam-report: EFV:NLI;SFV:NSPM;SFS:(7070007)(98901004);DIR:OUT;SFP:1901;SCL:1;SRVR:CY1NAM02HT209;H:BN1PR04MB842.namprd04.prod.outlook.com;FPR:;SPF:None;LANG:en; 5, 60 -- x-ms-office365-filtering-correlation-id: 5e516467-baa5-4412-8778-08d4e97f5ea1 5, 60 -- x-microsoft-antispam: UriScan:;BCL:0;PCL:0;RULEID:(300000500095)(300135000095)(300000501095)(300135300095)(22001)(300000502095)(300135100095)(300000503095)(300135400095)(201702061074)(5061506573)(5061507331)(1603103135)(2017031320274)(2017031324274)(2017031323274)(2017031322377)(1603101448)(1601125374)(1701031045)(300000504095)(300135200095)(300000505095)(300135600095)(300000506095)(300135500095);SRVR:CY1NAM02HT209; 5, 60 -- x-ms-traffictypediagnostic: CY1NAM02HT209: 5, 60 -- x-exchange-antispam-report-test: UriScan:(5213294742642)(148717330147763); 5, 60 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:(100000700101)(100105000095)(100000701101)(100105300095)(100000702101)(100105100095)(444000031);SRVR:CY1NAM02HT209;BCL:0;PCL:0;RULEID:(100000800101)(100110000095)(100000801101)(100110300095)(100000802101)(100110100095)(100000803101)(100110400095)(100000804101)(100110200095)(100000805101)(100110500095);SRVR:CY1NAM02HT209; 5, 60 -- x-forefront-prvs: 04073E895A 5, 60 -- spamdiagnosticoutput: 1:99 5, 60 -- spamdiagnosticmetadata: NSPM 5, 60 -- Content-Type: text/plain; charset="iso-8859-1" 5, 60 -- MIME-Version: 1.0 5, 60 -- X-OriginatorOrg: hotmail.com 5, 60 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 22 Aug 2017 17:01:02.2126 5, 60 -- (UTC) 5, 60 -- X-MS-Exchange-CrossTenant-fromentityheader: Internet 5, 60 -- X-MS-Exchange-CrossTenant-id: 84df9e7f-e9f6-40af-b435-aaaaaaaaaaaa 5, 60 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: CY1NAM02HT209 5, 60 -- Content-Transfer-Encoding: 8bit 5, 60 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v7MFvEZI023306 ==============================End of - Headers==============================
Hi, Sorry for not providing details in my original message. There are several research groups working on various projects with us. The best myelin preservation we have gotten so far is when the researcher used 4% PFA in phosphate buffer for perfusion. As soon as the sample is delivered to us (perhaps one to two hours later), we tried to trim down the specimen (~5mm x2mm) in PFA and transfer to 2% PFA/2.5% glutaraldehyde fixative and leave it overnight before processing. We have been using 1% osmium for 30 min and then reduced osmium for 30 min both on ice and 1% UA in water at room temp, gradually dehydrate up to 100% ETOH, then 100% acetone. and embed in AralditeEopon resin. We still see mild bubbling in 25 to 50% of myelin under this condition. I am wondering whether it would be better if we asked the researchers to transfer the specimen directly in a fixative containing glutaraldehyde immediately after dissecting out the tissue. Surgeries involving large animals are often very involved and costly. The researchers often need to collect a large number of tissue samples for multiple tests in limited time. We also do not have the luxuaries to perform trial experiments to test and optimize the best collecting and processing workflow. I hope some members of the list may have similar experience and can recommend best practice for fixation and sample collection under these constrained conditions.
Thank you for your input.
Ru-ching Hsia
________________________________________ X-from: Bentley, Karen {Karen_Bentley-at-URMC.Rochester.edu} Sent: Tuesday, August 22, 2017 1:54 PM To: hsia627-at-hotmail.com
X-from: jhyun-at-gatan.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jhyun-at-gatan.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: jhyun-at-gatan.com Name: John Hyun
Organization: Gatan
Title-Subject: [Filtered] EELS & EFTEM Analysis Training School - October 2017
Message: October 17-20, 2017 Pleasanton, CA, US
Electron energy loss spectroscopy (EELS) is a powerful technique that provides both compositional and chemical information from sub-nanometer areas in the sample. As a course attendee, you will learn best practices to set up and optimize your EELS hardware and experimental protocols so you can capture and extract the maximum amount of compositional and chemical information from your TEM samples. Topics include:
-Fundamentals of EELS and energy-filtered imaging in TEM -Principles of operation of EFTEM and EELS systems -Optimization of EFTEM and EELS data acquisition -Quantification of elemental composition -Other information provided by EFTEM/EELS and how best to extract it -Use of EELS signals to form maps of elemental and chemical composition -EFTEM and STEM EELS spectrum imaging techniques -Identification of material phases via EELS fine structure mapping -Applications to biological and physical science specimens
Thanks for your responses. As you suggested, I checked the diffusion pump heater and the pirani gauge, both looked fine. It turned out that the problem was a tiny bundle of dust fibers situated at the o ring sealing the specimen chamber. After some cleaning the scope goes vacuum ready but needs considerably more time, so I guess more cleaning may help further. Best regards yorgos
Hello Our Jeol SEM JSM5600LV doesn't get vacuum ready. Normally pre-evacuation and evacuation phases last 2' each, now pre-evacuation lasts 2' but evacuation phase seems endless After 1h no vacuum ready appeared neither any message from the software. Can be mechanical or electronic failure or both? Any help to tackle the p[problem will be greatly appreciated yorgos
In addition to JEMS, which Philippe Buffat described, there are also CSpot and Electron Diffraction. CSpot is commercial software that can help you index spot patterns, Kikuchi patterns, and ring patterns. There is a downloadable demo version. Electron Diffraction was written by Jean Paul Morniroli and can be downloaded for free. It does have problems with Rhombohedral crystal structure and he recommended that I use JEMS for B4C.
-Scott
-----Original Message----- X-from: Z.Zhou-at-lboro.ac.uk [mailto:Z.Zhou-at-lboro.ac.uk] Sent: Friday, August 18, 2017 5:34 AM To: S.Walck-at-comcast.net
Dear Fellow Microscopists, How do you index electron diffraction patterns recorded by TEM/SAED? Especially for those likely to be of hexagonal, orthorhombic or tetragonal etc. complex crystal structure? I'm looking for some software to aid pattern indexing, commercial or free as long as they are reliable and easy to use. Any suggestions would be highly appreciated please... Zhou
Dr Z Zhou Research Fellow Loughborough Materials Characterisation Centre Department of Materials Loughborough University Loughborough LE11 3TU UK 01509 223163
==============================Original Headers============================== 4, 79 -- From Z.Zhou-at-lboro.ac.uk Fri Aug 18 04:17:04 2017 4, 79 -- Received: from mta-2.lboro.ac.uk (mta-2.lut.ac.uk [158.125.160.48]) 4, 79 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v7I9H33x004524 4, 79 -- for {Microscopy-at-microscopy.com} ; Fri, 18 Aug 2017 04:17:04 -0500 4, 79 -- Received: from [158.125.160.47] (helo=mta-1.lboro.ac.uk) 4, 79 -- by mta-2.lboro.ac.uk with esmtps (TLSv1.2:ECDHE-RSA-AES256-GCM-SHA384:256) 4, 79 -- (Exim 4.89) 4, 79 -- id 1didPN-0004T0-Te 4, 79 -- for Microscopy-at-microscopy.com; Fri, 18 Aug 2017 10:17:37 +0100 4, 79 -- Received: from [158.125.160.17] (helo=itsex-2.lunet.lboro.ac.uk) 4, 79 -- by mta-1.lboro.ac.uk with esmtps (TLSv1.2:ECDHE-RSA-AES256-SHA384:256) 4, 79 -- (Exim 4.89) 4, 79 -- id 1didPL-00077J-DC 4, 79 -- for Microscopy-at-microscopy.com; Fri, 18 Aug 2017 10:17:35 +0100 4, 79 -- Received: from itsex-2.lunet.lboro.ac.uk 4, 79 -- (2001:630:301:d314:580f:144d:cf5e:c130) by itsex-2.lunet.lboro.ac.uk 4, 79 -- (2001:630:301:d314:580f:144d:cf5e:c130) with Microsoft SMTP Server 4, 79 -- (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_256_CBC_SHA384_P384) id 4, 79 -- 15.1.669.32; Fri, 18 Aug 2017 10:17:35 +0100 4, 79 -- Received: from EUR02-AM5-obe.outbound.protection.outlook.com (213.199.180.146) 4, 79 -- by itsex-2.lunet.lboro.ac.uk (158.125.160.17) with Microsoft SMTP Server 4, 79 -- (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_256_CBC_SHA384_P384) id 4, 79 -- 15.1.669.32 via Frontend Transport; Fri, 18 Aug 2017 10:17:35 +0100 4, 79 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=lunet.onmicrosoft.com; 4, 79 -- s=selector1-lboro-ac-uk; 4, 79 -- h=From:Date:Subject:Message-ID:Content-Type:MIME-Version; 4, 79 -- bh=INmiBzyU4DXdy8AZUWC/bLI3qkU6ji3TUqt7IumQmI0=; 4, 79 -- b=WCVIoSuWPtf97kNZYulwiQMa+Ilw1G62i5/jQ6ql/3GjYS6ya4+1nG37df5jAuMjPo749hytEA hrb4yoqBPo26IhrosTelltSDn5cxIgK3Vq1GPCxAkprPn8VhIPjxPAPOPCv1V9WhdasjZy5vpEZz ZwETCY1FMYZOZPTrQ9SBc= 4, 79 -- Received: from AM5PR0402MB2771.eurprd04.prod.outlook.com (10.175.41.13) by 4, 79 -- AM5PR0402MB2818.eurprd04.prod.outlook.com (10.175.41.137) with
There is another available software that TEM users might be interested.
Landyne software suit can used for simulation and index for spot and ring SAED patterns (http://www.unl.edu\ncmn-cfem/xzli\computer-programs).
Yan Xin NHMFL/FSU
-----Original Message----- X-from: philippe.buffat-at-epfl.ch [mailto:philippe.buffat-at-epfl.ch] Sent: Friday, August 18, 2017 8:57 AM To: xin-at-magnet.fsu.edu
Dr Z Zhou asked: How do you index electron diffraction patterns recorded by TEM/SAED?
You may consider JEMS from P. Stadelmann. Have a look to http://www.jems-saas.ch/ that also contain the access to the limited "Student version" for demo on Mac, PC and Linux.
This software is a comprehensive program for (HR) TEM+STEM image simulation and electron diffraction (SAED, nano-diff, CBED, Kikuchi, precession, powder patterns) interpretation and simulation based on Bloch waves and multislice approaches.
In the present case, feeding JEMS with files containing the crystal parameters for all suspected phases allows an automatic match with the experimental patterns for phase identification.
Disclaimer: P. Stadelmann and myself were working in the same laboratory for long though on different subjects.
P. Buffat
==============================Original Headers============================== 7, 22 -- From philippe.buffat-at-epfl.ch Fri Aug 18 07:55:59 2017 7, 22 -- Received: from smtp5.epfl.ch (smtp5.epfl.ch [128.178.224.8]) 7, 22 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v7ICtwvg001840 7, 22 -- for {microscopy-at-microscopy.com} ; Fri, 18 Aug 2017 07:55:58 -0500 7, 22 -- Received: (qmail 30134 invoked by uid 107); 18 Aug 2017 12:57:59 -0000 7, 22 -- X-Virus-Scanned: ClamAV 7, 22 -- Received: from 133.93.198.178.dynamic.wline.res.cust.swisscom.ch (HELO cimepc22.home) (178.198.93.133) (TLS, ECDHE-RSA-AES256-GCM-SHA384 (X25519 curve) cipher) (authenticated) 7, 22 -- by mail.epfl.ch (AngelmatoPhylax SMTP proxy) with ESMTPSA; Fri, 18 Aug 2017 14:57:59 +0200 7, 22 -- X-EPFL-Auth: uGuFJeSWI5fynBn8zaffRvlpYA/tffNLfQSLtsxFCKIq5Kjxfnw= 7, 22 -- Reply-To: philippe.buffat-at-epfl.ch 7, 22 -- To: Microscopy-at-microscopy.com 7, 22 -- From: Philippe Buffat {philippe.buffat-at-epfl.ch} 7, 22 -- Organization: EPFL 7, 22 -- Subject: TEM: softwares to aid selected area diffraction patterns indexing 7, 22 -- Message-ID: {4b078e8c-7ca4-543c-ec88-f5ac88a41de2-at-epfl.ch} 7, 22 -- Date: Fri, 18 Aug 2017 14:57:58 +0200 7, 22 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.10; rv:52.0) 7, 22 -- Gecko/20100101 Thunderbird/52.2.1 7, 22 -- MIME-Version: 1.0 7, 22 -- Content-Type: text/plain; charset=utf-8; format=flowed 7, 22 -- Content-Transfer-Encoding: 7bit 7, 22 -- Content-Language: en-US ==============================End of - Headers==============================
==============================Original Headers============================== 15, 116 -- From xin-at-magnet.fsu.edu Wed Aug 23 09:36:22 2017 15, 116 -- Received: from NAM01-BY2-obe.outbound.protection.outlook.com (mail-by2nam01on0070.outbound.protection.outlook.com [104.47.34.70]) 15, 116 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v7NEaKN1012124 15, 116 -- for {Microscopy-at-microscopy.com} ; Wed, 23 Aug 2017 09:36:21 -0500 15, 116 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=fsu.onmicrosoft.com; 15, 116 -- s=selector1-magnet-fsu-edu; 15, 116 -- h=From:Date:Subject:Message-ID:Content-Type:MIME-Version; 15, 116 -- bh=yTEZ+HRRuk9NXgkZD9fyalMrZPMwcKRiuDq6IOlFLUk=; 15, 116 -- b=Ylv++EHjC4EwJG8aPT1h3ZyNfUPMlnXO5boadAZvgmnXCVxqkM2nf//IrOBQfADROQQDIgwwj3IdFT6rptXX56scIjF6C01jxO1JlG9wgwNpxVNYPO8hLRPLdz23xcXQLXnS15J68Hne07ftlqRwUZJhVAhcL0FHqBRXiE1fsvM= 15, 116 -- Received: from MWHPR02CA0060.namprd02.prod.outlook.com (10.164.133.49) by 15, 116 -- CY4PR02MB3303.namprd02.prod.outlook.com (10.165.88.162) with Microsoft SMTP 15, 116 -- Server (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_128_CBC_SHA256_P256) id 15, 116 -- 15.1.1362.18; Wed, 23 Aug 2017 15:39:58 +0000 15, 116 -- Received: from BN1BFFO11OLC004.protection.gbl (2a01:111:f400:7c10::1:173) by 15, 116 -- MWHPR02CA0060.outlook.office365.com (2603:10b6:301:60::49) with Microsoft 15, 116 -- SMTP Server (version=TLS1_2, 15, 116 -- cipher=TLS_ECDHE_RSA_WITH_AES_128_CBC_SHA256_P256) id 15.1.1362.18 via 15, 116 -- Frontend Transport; Wed, 23 Aug 2017 15:39:58 +0000 15, 116 -- Authentication-Results: spf=neutral (sender IP is 146.201.107.249) 15, 116 -- smtp.mailfrom=magnet.fsu.edu; microscopy.com; dkim=none (message not signed) 15, 116 -- header.d=none;microscopy.com; dmarc=none action=none 15, 116 -- header.from=magnet.fsu.edu; 15, 116 -- Received-SPF: Neutral (protection.outlook.com: 146.201.107.249 is neither 15, 116 -- permitted nor denied by domain of magnet.fsu.edu) 15, 116 -- Received: from mailrelay04.its.fsu.edu (146.201.107.249) by 15, 116 -- BN1BFFO11OLC004.mail.protection.outlook.com (10.58.145.15) with Microsoft 15, 116 -- SMTP Server (version=TLS1_2, 15, 116 -- cipher=TLS_ECDHE_RSA_WITH_AES_256_CBC_SHA384_P384) id 15.1.1341.15 via 15, 116 -- Frontend Transport; Wed, 23 Aug 2017 15:39:57 +0000 15, 116 -- Received: from zm1.magnet.fsu.edu (mail.magnet.fsu.edu [146.201.250.62]) 15, 116 -- by mailrelay04.its.fsu.edu (8.14.7/8.14.7) with ESMTP id v7NFduP0018871; 15, 116 -- Wed, 23 Aug 2017 11:39:56 -0400 15, 116 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 15, 116 -- by zm1.magnet.fsu.edu (Postfix) with ESMTP id B40DE30BA088; 15, 116 -- Wed, 23 Aug 2017 11:39:56 -0400 (EDT) 15, 116 -- Received: from zm1.magnet.fsu.edu ([127.0.0.1]) 15, 116 -- by localhost (zm1.magnet.fsu.edu [127.0.0.1]) (amavisd-new, port 10032) 15, 116 -- with ESMTP id FgYtmEvL3wpn; Wed, 23 Aug 2017 11:39:56 -0400 (EDT) 15, 116 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 15, 116 -- by zm1.magnet.fsu.edu (Postfix) with ESMTP id 943D930BA08B; 15, 116 -- Wed, 23 Aug 2017 11:39:56 -0400 (EDT) 15, 116 -- Received: from zm1.magnet.fsu.edu ([127.0.0.1]) 15, 116 -- by localhost (zm1.magnet.fsu.edu [127.0.0.1]) (amavisd-new, port 10026) 15, 116 -- with ESMTP id vhmj5BJSbvPC; Wed, 23 Aug 2017 11:39:56 -0400 (EDT) 15, 116 -- Received: from zm1.magnet.fsu.edu (zm1.magnet.fsu.edu [146.201.250.62]) 15, 116 -- by zm1.magnet.fsu.edu (Postfix) with ESMTP id 77E8E30BA088; 15, 116 -- Wed, 23 Aug 2017 11:39:56 -0400 (EDT) 15, 116 -- From: Yan Xin {xin-at-magnet.fsu.edu} 15, 116 -- To: {philippe.buffat-at-epfl.ch} , {Microscopy-at-microscopy.com} 15, 116 -- References: {201708181256.v7ICujVR002464-at-microscopy.com} 15, 116 -- In-Reply-To: {201708181256.v7ICujVR002464-at-microscopy.com} 15, 116 -- Subject: RE: [Microscopy] TEM: softwares to aid selected area diffraction patterns indexing 15, 116 -- Date: Wed, 23 Aug 2017 11:39:55 -0400 15, 116 -- Message-ID: {7aaea007.00000e14.0000000c-at-xin-980.ad.magnet.fsu.edu} 15, 116 -- MIME-Version: 1.0 15, 116 -- Content-Type: text/plain; charset="us-ascii" 15, 116 -- Content-Transfer-Encoding: 7bit 15, 116 -- X-Mailer: Microsoft Office Outlook 12.0 15, 116 -- X-Mailer: Zimbra 8.6.0_GA_1200 (ZimbraConnectorForOutlook/7.2.1.530) 15, 116 -- Thread-Index: eGFKewLU4k7dbGAddJtwzU7KPl0b/Q== 15, 116 -- Content-Language: en-us 15, 116 -- Thread-Topic: softwares to aid selected area diffraction patterns indexing 15, 116 -- X-EOPAttributedMessage: 0 15, 116 -- X-Forefront-Antispam-Report: 15, 116 -- CIP:146.201.107.249;IPV:CAL;SCL:-1;CTRY:US;EFV:NLI;SFV:NSPM;SFS:(10009020)(6009001)(39860400002)(2980300002)(199003)(13464003)(189002)(377454003)(53546010)(7596002)(189998001)(966005)(23726003)(102836003)(88526003)(8676002)(305945005)(1720100001)(478600001)(8936002)(229853002)(26826003)(50226002)(47776003)(2906002)(230700001)(5660300001)(50986999)(76176999)(109096001)(4980500002)(33646002)(46406003)(2950100002)(42882006)(86362001)(14726001)(105586002)(246002)(97756001)(88552002)(6306002)(61296003)(75432002)(6266002)(6246003)(626005)(106466001)(356003)(50466002)(2690400003)(18906007);DIR:OUT;SFP:1101;SCL:1;SRVR:CY4PR02MB3303;H:mailrelay04.its.fsu.edu;FPR:;SPF:Neutral;PTR:mailrelay04.its.fsu.edu;A:1;MX:1;LANG:en; 15, 116 -- X-Microsoft-Exchange-Diagnostics: 1;BN1BFFO11OLC004;1:iMw6YJQbXyYRPxuuPljK0v58CDixpobQSpAW33cojQxLabtaj1rtE9BaJ1b8PHfRmKG6c2XrrSPH2y63GUbFBdiyNVpUM8BWA1oAa4DNC3lAFDQnREbVuEs7MR1eHkzB 15, 116 -- X-MS-PublicTrafficType: Email 15, 116 -- X-MS-Office365-Filtering-Correlation-Id: cbd78c9a-84a5-4222-4229-08d4ea3d3580 15, 116 -- X-Microsoft-Antispam: 15, 116 -- UriScan:;BCL:0;PCL:0;RULEID:(300000500095)(300135000095)(300000501095)(300135300095)(22001)(300000502095)(300135100095)(2017030254152)(300000503095)(300135400095)(2017052603031)(201703131423075)(201703031133081)(201702281549075)(300000504095)(300135200095)(300000505095)(300135600095)(300000506095)(300135500095);SRVR:CY4PR02MB3303; 15, 116 -- X-Microsoft-Exchange-Diagnostics: 15, 116 -- 1;CY4PR02MB3303;3:eeL23ZKhTbV9SAs97TJHqyf+aWsSsF7ypgE6GjvBAXOni71QMMYRK4jouE8hl2slOt3kkGlrS2KpbbGwnXzCaGd9KYHOJDEykOBXZXw+m8AcXvb7hpZVI+U/6xWTjQ8Dx8N+M9lI+3MwyCIaxPkb4maH6N5Mtko92LROAjaFmlhzQLwZwBPR0zI4trSpiHVwTSaAUKW+hvMyVr7F6nz0S1D8I+SYtQ/qcyMIIrrf5XUXmq5ygg9usZoj12sngs2AKIBOXY64rG3paupPaPZsVbmnLhCsy0vqW+7azt3ylaGrmP7laYoM52uYXcDz0yHBGZmFz6JCCD0LrPTAQlfaHQpfeAtZZkvC+g5Oudoo/U8=;25:KeJKF35SOGsimNJpc8AazCFYf03+O3koa56pXQ4WNkzppLNMdxatykm19g0krvvNaBAP9ceXil971bTtIPKc+X5NDjXqgpDG0uehzySqyTPB1sSs/PxqM0C7LzVXt8WiVE7t6L7UbX3ygXqYjTM2K7V91oce0HWVY6HZxV8wiHuH0Nk6bSnD5hIKXWn+SG4oHn5QxSG97Y3QKXNy+qs0jiHykRJWgXTkYvjqBTDDUKSRpFzOYrhCSsg4n/wwVvF9yFD7rBEKyEuzpCMtJEDZ1CWq211NROYx7MNMSnZKMdXs/T9Q22j1AHAGEp8oIjNTe02cN+kkAiJsb8pcSc0bmA== 15, 116 -- X-MS-TrafficTypeDiagnostic: CY4PR02MB3303: 15, 116 -- X-Microsoft-Exchange-Diagnostics: 15, 116 -- 1;CY4PR02MB3303;31:gEa9VIz+OtWrvDJgzw/K3/XVom2mq/XP8nnsWpP3Gcs/bJilxgF1eWVOHS6gDXPh8GFfes34TLgFLAf7Fka+6W5Q5GDF8A7Ru3v0OAweFS4lfIGTAiCs+2vjr/SasWWW62RExSQ9JiXOT42ydKMxGUWXaW/vmU/yb7cCjashVV5y9P3EvMxl6tffMmA49/DnpjhXDyGLa6WDBOO+4wYQaZbTL/OyIWASYDtG8FDfVjI=;20: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 15, 116 -- X-Exchange-Antispam-Report-Test: UriScan:(129945304726314)(180861224563641); 15, 116 -- X-Microsoft-Antispam-PRVS: 15, 116 -- {CY4PR02MB33031C06AF9B4C39BCCFFB8C85850-at-CY4PR02MB3303.namprd02.prod.outlook.com} 15, 116 -- X-Exchange-Antispam-Report-CFA-Test: 15, 116 -- BCL:0;PCL:0;RULEID:(100000700101)(100105000095)(100000701101)(100105300095)(100000702101)(100105100095)(6040450)(601004)(2401047)(13013025)(5005006)(8121501046)(13021025)(100000703101)(100105400095)(93006095)(93003095)(10201501046)(3002001)(6041248)(20161123564025)(20161123558100)(20161123562025)(20161123555025)(201703131423075)(201702281529075)(201702281528075)(201703061421075)(201703061406153)(20161123560025)(6072148)(201708071742011)(100000704101)(100105200095)(100000705101)(100105500095);SRVR:CY4PR02MB3303;BCL:0;PCL:0;RULEID:(100000800101)(100110000095)(100000801101)(100110300095)(100000802101)(100110100095)(100000803101)(100110400095)(100000804101)(100110200095)(100000805101)(100110500095);SRVR:CY4PR02MB3303; 15, 116 -- X-Microsoft-Exchange-Diagnostics: 15, 116 -- 1;CY4PR02MB3303;4:7JlZoQUcPet2Tl+6t+JqKhfXAbNTrR5vBIPqMKB0hrCayu4gRwWm+BlptqPbIBuhEntp0drB00Ou3xXxLeBN0QwMWO9Ot3vczBSReOAHGaTIrWx8rvRb1HyXEdWCrLSnBdLl1CLgK+fEk97kqYBhysbDeZSPTiNdzFTqSYT2RsMJWAI+OQaQfD7EX6ozOzqhqVddICQA6QUb5YnyMhtr6lXzUE91GA8wkOYVzlThTXIaczSRW2kmRy/fXzLZk4vHTG+OCKHmOxntJ1WdqaCM+udPTYzyeX7BCO/iBde3HSkhvRYE6zmeUGkn/Z8q2BCwvKx2QlZ5DjLUaEeAh3t+tg== 15, 116 -- X-Forefront-PRVS: 040866B734 15, 116 -- X-Microsoft-Exchange-Diagnostics: 15, 116 -- =?us-ascii?Q?1;CY4PR02MB3303;23:qtaP6woZnE3vFWGaWFKpLA9D52NTxnkr+CJze++jm?= 15, 116 -- =?us-ascii?Q?Rc04XcVjR6uo8oUOT5lqMBXFgGZXCp72+fr/HnQq7Nltd+Bj82huR/GacaBc?= 15, 116 -- =?us-ascii?Q?KtS2+2MulCiRpbIfkDrB4nyVG5IMF1f36A3QJek4RGDI9h61Gu47QHCxeTz2?= 15, 116 -- =?us-ascii?Q?K9Lhj6ZFzcxohhFi/rSITJ19nWyZjzSpLu6u0x1BDiavX+ENzlWiphbqYf0q?= 15, 116 -- =?us-ascii?Q?OW5eUaowHx6n9ut8/obwpZPzpV4U9H5/D2eflXIUkpeR6uUOo9dqGYrC7CoR?= 15, 116 -- =?us-ascii?Q?ou/9XthUrKC+TCGZc7kZAMHf9tS9ISGKI9JB1EMSzjum/sPgpDFidERSPJ4Y?= 15, 116 -- =?us-ascii?Q?QqDtw+yLIDjYL73yIpWoelHP54Pz9hftPEo5n86AA/5YXUjQ2qjpgC+7OKLD?= 15, 116 -- =?us-ascii?Q?zBU9tdbQN1pHxjZ8L2MFqYtl65XWi5Etq3eiw668Ifwz76DgJ2xX1P9kPFEs?= 15, 116 -- =?us-ascii?Q?2DW2gXd6k7nK/9ssEK5fpuFJvW8Lo/j5AGu3jlzgidDWHX47sKn1BwFRS4pv?= 15, 116 -- =?us-ascii?Q?DeLbqgZV0NEF/TBzRT7Sesbobi9uPkA6UJ12Mg33cRS4rMO65srYSFk0zDmL?= 15, 116 -- =?us-ascii?Q?EkzbDWWZR1nAbmzlMXmI1CaMlBXH922VkJ3gSakqd0s1DNxwlsqmCeHrNSUk?= 15, 116 -- =?us-ascii?Q?3gwIRhsH1EUmSq7B449h1opRg/jqHRfnNdxUX73vC5MmiEA4hX7zE8UTi4hi?= 15, 116 -- =?us-ascii?Q?v3GYKmV8Ff/vM1mECLAs32i2P+GsBEP+Yqvb3HKFTyqoj7fPOjAEfhXGD3Uz?= 15, 116 -- =?us-ascii?Q?TgFi6YdETi8ii6dGKZ7twbGq7lvQbs7k1D6k9Ce6GQVz2RNFuFMiVpsLoK65?= 15, 116 -- =?us-ascii?Q?FmxSRG8my/4bYE8IVRFMT2hmSCUr5LT0n112tEABci+PIM9RNlmPp18diTPb?= 15, 116 -- =?us-ascii?Q?LnDhEIFmAXrHz4rQiAOoN1dr8HBgermp2r/6H0m8bhHuvR5/HoFJCyOvw+Jn?= 15, 116 -- =?us-ascii?Q?KXvU4jla6Jh0g0VK8K4WyiqrSokYeX9Tr5Gtuvqn5b1ZudlXclcZwRIj/1by?= 15, 116 -- =?us-ascii?Q?wY66k5Cn8aC/4AqnOMozuIq9caaDosf4APM5kQVsaNalPvZ9P9Pq8FPGCGIK?= 15, 116 -- =?us-ascii?Q?OEtfMqJKQ04CjNN8BgQ3VhpLMtHPD8M31TAst2xNLqanp+sMeFeCtr69FQLO?= 15, 116 -- =?us-ascii?Q?vMUHU3AraV3HtImWy09w4m0xZF8gfHaArGuCHff9t1fBqPK5U0LxSl9vdjjy?= 15, 116 -- =?us-ascii?Q?Y2BwrRzgW807tii9be0kfcrv9R8FYKg7GJs?= 15, 116 -- X-Microsoft-Exchange-Diagnostics: 15, 116 -- 1;CY4PR02MB3303;6:/rWtX1QEgnIxdpMK8fSBfPVDZChaMPJJLJZX/ge/4i7B6JW+k59AQKn1fDJ7kRrucY91CrFTB7wQtdJLBlRjCxWMkKojUUmk5HpG2B8D1S9QLZWKdM13eus2SIDj3S7V4lLv9KC4JwOkeKISHHVwytgrnatY4XqQPgUBGkeVU7YLtlihPOTyeNfKR3N9PuzBulwh5ELg59cQF2ZvBbznqG4QmXtshKqSTBPBIKIXe4LCSvL/syOzPz/7+EIIsahLJGxAWvuGOwgLxk16SHG8s9ybf70EMPmX6j5Uflp68bzL34v1sl9vUPo7/CUkowDWiSPf87U4kTY4K7tSjUjYyw==;5:/p2VK1ataPf0pe155GnuzkHp4PwY+YlSsDMHW6V7il+CllSNutPTJ/rVpLwQ5bpij/rtQVZe5kWx2q9AjUk5EGWttUMX30s1XhKeaO0yltY1b+guBafm/b5SkbLeqR7gmzhkWOLQnL52C9j9GIRFwA==;24:MAcC2+4wxKm7VRAF0nB/ctE8oLi20TP5F8Zd0o4+fbdyRQ4YuX3EbI1A+G8sXifXqHGa2EY6cdpDkI9ZgSkqFPkPL3xE7sAwEg96eTZm5yM=;7:SEUcY3atoRek70B6d4IkTL6onoFvxQ+dUXmEzoexwRyRviTL/SRY7n6w1suwLIm3LlDNRnDyYRu5yGUbhPz3HrsFeUfJavpM7GtIcg3FdD6U5RDdOTJIe0gcNY5EbiSplqTZQqpXm+oDHHitbcdiK2CJQzupqApRRWLZCBLdRaD67yEjR2ip/uYPUc0bAp7/mC2++xPA0t8Juc37VGlmjNLeDsaZO2GXndrDs6M7hec= 15, 116 -- SpamDiagnosticOutput: 1:99 15, 116 -- SpamDiagnosticMetadata: NSPM 15, 116 -- X-OriginatorOrg: magnet.fsu.edu 15, 116 -- X-MS-Exchange-CrossTenant-OriginalArrivalTime: 23 Aug 2017 15:39:57.6337 15, 116 -- (UTC) 15, 116 -- X-MS-Exchange-CrossTenant-Id: a36450eb-db06-42a7-8d1b-026719f701e3 15, 116 -- X-MS-Exchange-CrossTenant-OriginalAttributedTenantConnectingIp: TenantId=a36450eb-db06-42a7-8d1b-026719f701e3;Ip=[146.201.107.249];Helo=[mailrelay04.its.fsu.edu] 15, 116 -- X-MS-Exchange-CrossTenant-FromEntityHeader: HybridOnPrem 15, 116 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: CY4PR02MB3303 ==============================End of - Headers==============================
There is another available software that TEM users might be interested.
Landyne software suit can used for simulation and index for spot and ring SAED patterns (http://www.unl.edu\ncmn-cfem/xzli\computer-programs).
Yan Xin NHMFL/FSU
-----Original Message----- X-from: philippe.buffat-at-epfl.ch [mailto:philippe.buffat-at-epfl.ch] Sent: Friday, August 18, 2017 8:57 AM To: xin-at-magnet.fsu.edu
Dr Z Zhou asked: How do you index electron diffraction patterns recorded by TEM/SAED?
You may consider JEMS from P. Stadelmann. Have a look to http://www.jems-saas.ch/ that also contain the access to the limited "Student version" for demo on Mac, PC and Linux.
This software is a comprehensive program for (HR) TEM+STEM image simulation and electron diffraction (SAED, nano-diff, CBED, Kikuchi, precession, powder patterns) interpretation and simulation based on Bloch waves and multislice approaches.
In the present case, feeding JEMS with files containing the crystal parameters for all suspected phases allows an automatic match with the experimental patterns for phase identification.
Disclaimer: P. Stadelmann and myself were working in the same laboratory for long though on different subjects.
P. Buffat
==============================Original Headers============================== 16, 116 -- From xin-at-magnet.fsu.edu Wed Aug 23 09:42:53 2017 16, 116 -- Received: from NAM03-DM3-obe.outbound.protection.outlook.com (mail-dm3nam03on0060.outbound.protection.outlook.com [104.47.41.60]) 16, 116 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v7NEgr7Z017522 16, 116 -- for {Microscopy-at-microscopy.com} ; Wed, 23 Aug 2017 09:42:53 -0500 16, 116 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=fsu.onmicrosoft.com; 16, 116 -- s=selector1-magnet-fsu-edu; 16, 116 -- h=From:Date:Subject:Message-ID:Content-Type:MIME-Version; 16, 116 -- bh=wLeLAMeWodhwO+OS7VEj48D/AI/ILhvw/rzjImlnYvM=; 16, 116 -- b=f6vnURe8yMJpx+R1QZzxm2MJFhuWht+cAye7oJXQUXNFK5rf+2k9A9ifWMU637KdvVCcUthZCENs3rhuhE0xlTNGEsD1fHxXOedeT/clLgJME6ig1NpgNUHIBzcHybU2gg+/cOb3/7kS3IM/O2rIs7z1nbrnONRmsOI33/+GPuo= 16, 116 -- Received: from CY4PR02CA0040.namprd02.prod.outlook.com (10.175.57.154) by 16, 116 -- CY4PR02MB3304.namprd02.prod.outlook.com (10.165.88.163) with Microsoft SMTP 16, 116 -- Server (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_256_CBC_SHA384_P256) id 16, 116 -- 15.1.1362.18; Wed, 23 Aug 2017 15:46:43 +0000 16, 116 -- Received: from BL2FFO11FD022.protection.gbl (2a01:111:f400:7c09::126) by 16, 116 -- CY4PR02CA0040.outlook.office365.com (2603:10b6:903:117::26) with Microsoft 16, 116 -- SMTP Server (version=TLS1_2, 16, 116 -- cipher=TLS_ECDHE_RSA_WITH_AES_256_CBC_SHA384_P256) id 15.1.1385.8 via 16, 116 -- Frontend Transport; Wed, 23 Aug 2017 15:46:43 +0000 16, 116 -- Authentication-Results: spf=neutral (sender IP is 146.201.107.249) 16, 116 -- smtp.mailfrom=magnet.fsu.edu; microscopy.com; dkim=none (message not signed) 16, 116 -- header.d=none;microscopy.com; dmarc=none action=none 16, 116 -- header.from=magnet.fsu.edu; 16, 116 -- Received-SPF: Neutral (protection.outlook.com: 146.201.107.249 is neither 16, 116 -- permitted nor denied by domain of magnet.fsu.edu) 16, 116 -- Received: from mailrelay04.its.fsu.edu (146.201.107.249) by 16, 116 -- BL2FFO11FD022.mail.protection.outlook.com (10.173.161.101) with Microsoft 16, 116 -- SMTP Server (version=TLS1_2, 16, 116 -- cipher=TLS_ECDHE_RSA_WITH_AES_256_CBC_SHA384_P384) id 15.1.1341.15 via 16, 116 -- Frontend Transport; Wed, 23 Aug 2017 15:46:43 +0000 16, 116 -- Received: from zm1.magnet.fsu.edu (mail.magnet.fsu.edu [146.201.250.62]) 16, 116 -- by mailrelay04.its.fsu.edu (8.14.7/8.14.7) with ESMTP id v7NFkgwf019687 16, 116 -- for {Microscopy-at-microscopy.com} ; Wed, 23 Aug 2017 11:46:42 -0400 16, 116 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 16, 116 -- by zm1.magnet.fsu.edu (Postfix) with ESMTP id 9E9EF30BA088 16, 116 -- for {Microscopy-at-microscopy.com} ; Wed, 23 Aug 2017 11:46:42 -0400 (EDT) 16, 116 -- Received: from zm1.magnet.fsu.edu ([127.0.0.1]) 16, 116 -- by localhost (zm1.magnet.fsu.edu [127.0.0.1]) (amavisd-new, port 10032) 16, 116 -- with ESMTP id il_XhzMlsCNk for {Microscopy-at-microscopy.com} ; 16, 116 -- Wed, 23 Aug 2017 11:46:42 -0400 (EDT) 16, 116 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 16, 116 -- by zm1.magnet.fsu.edu (Postfix) with ESMTP id 7F83530BA08B 16, 116 -- for {Microscopy-at-microscopy.com} ; Wed, 23 Aug 2017 11:46:42 -0400 (EDT) 16, 116 -- Received: from zm1.magnet.fsu.edu ([127.0.0.1]) 16, 116 -- by localhost (zm1.magnet.fsu.edu [127.0.0.1]) (amavisd-new, port 10026) 16, 116 -- with ESMTP id ztTA1bxproVR for {Microscopy-at-microscopy.com} ; 16, 116 -- Wed, 23 Aug 2017 11:46:42 -0400 (EDT) 16, 116 -- Received: from zm1.magnet.fsu.edu (zm1.magnet.fsu.edu [146.201.250.62]) 16, 116 -- by zm1.magnet.fsu.edu (Postfix) with ESMTP id 68D9E30BA088 16, 116 -- for {Microscopy-at-microscopy.com} ; Wed, 23 Aug 2017 11:46:42 -0400 (EDT) 16, 116 -- From: Yan Xin {xin-at-magnet.fsu.edu} 16, 116 -- To: {Microscopy-at-microscopy.com} 16, 116 -- Subject: RE: TEM: softwares to aid selected area diffraction patterns indexing 16, 116 -- Date: Wed, 23 Aug 2017 11:46:42 -0400 16, 116 -- Message-ID: {d7e83b11.00000e14.00000014-at-xin-980.ad.magnet.fsu.edu} 16, 116 -- MIME-Version: 1.0 16, 116 -- Content-Type: text/plain; charset="us-ascii" 16, 116 -- Content-Transfer-Encoding: 7bit 16, 116 -- X-Mailer: Microsoft Office Outlook 12.0 16, 116 -- X-Mailer: Zimbra 8.6.0_GA_1200 (ZimbraConnectorForOutlook/7.2.1.530) 16, 116 -- Thread-Index: pRlbTQojDxLq6xdFKztwTfOGSr0qDw== 16, 116 -- Content-Language: en-us 16, 116 -- Thread-Topic: softwares to aid selected area diffraction patterns indexing 16, 116 -- X-EOPAttributedMessage: 0 16, 116 -- X-Forefront-Antispam-Report: 16, 116 -- CIP:146.201.107.249;IPV:CAL;SCL:-1;CTRY:US;EFV:NLI;SFV:NSPM;SFS:(10009020)(6009001)(39860400002)(2980300002)(189002)(13464003)(199003)(377454003)(246002)(75432002)(7596002)(229853002)(88552002)(14726001)(2906002)(5660300001)(102836003)(33646002)(105586002)(478600001)(26826003)(305945005)(109096001)(4980500002)(2351001)(97756001)(47776003)(356003)(61296003)(50986999)(6266002)(106466001)(50466002)(1720100001)(42882006)(86362001)(23726003)(966005)(230700001)(6916009)(8676002)(8936002)(110136004)(6246003)(626005)(50226002)(6306002)(46406003)(189998001)(88526003)(2690400003);DIR:OUT;SFP:1101;SCL:1;SRVR:CY4PR02MB3304;H:mailrelay04.its.fsu.edu;FPR:;SPF:Neutral;PTR:mailrelay04.its.fsu.edu;MX:1;A:1;LANG:en; 16, 116 -- X-Microsoft-Exchange-Diagnostics: 1;BL2FFO11FD022;1:7+X/QTYqvz+Nbh0EVgwZU6vIRveDQDbb5j67SMp4dJ8N1XGtOXO6y8oZYFU9K6usupaIqcTRUEu+Ki0ZSIv1bWT8D2pIMgtpGd3rb3twLDmhrbYQknVTTIFBBiPUMG6N 16, 116 -- X-MS-PublicTrafficType: Email 16, 116 -- X-MS-Office365-Filtering-Correlation-Id: a07465c6-92dc-4056-68b3-08d4ea3e272b 16, 116 -- X-Microsoft-Antispam: 16, 116 -- UriScan:;BCL:0;PCL:0;RULEID:(300000500095)(300135000095)(300000501095)(300135300095)(22001)(300000502095)(300135100095)(2017030254152)(300000503095)(300135400095)(2017052603031)(201703131423075)(201703031133081)(201702281549075)(300000504095)(300135200095)(300000505095)(300135600095)(300000506095)(300135500095);SRVR:CY4PR02MB3304; 16, 116 -- X-Microsoft-Exchange-Diagnostics: 16, 116 -- 1;CY4PR02MB3304;3:5wTUYGbreDFQL2fAq7FwfeOGjplGzhciQocK0NywJ7iLmg/9NQvty/PzE4V0LhaW4LSRf494kzjJuVZnqmXsmoNBtA1HX+t4J/l4tJakMmrctOvaFea14Z4f548Aoiv2bP5F0ByYl35DwkF+dRtRVjRJFUDRLrjzP6RPA9Ix85y6qeZly1qEmw5qidsXggi/dyK9+HWtXBpCgmzPVjsN3eXgETE/JOF72ujtEYEwPUyv6wKjNBTV8TDS+MqsRtHWHHGEHMDrUOa51LaY8zhp8yF2FkqPNV27nLVsZbeLdLL5LwHM+hka6DVSnRiVMREHl+1T3aaMZsrICLdfF+MhDOYzJSjwU89UltdPTXSrvnU=;25:4txeSkYCTGD1dYKooCXl3EEyEhxqb2ymnwD464rxCmjiwUL+ntEh5wEK8PQljWkxYx60TpLu8L0TCOY44o6cG7Gy77XQaaeCerjPptESUKPnRxAYRHBXe7ZFc5ymi9dHyWATsB/xmn3BGvJf1MGaDJlfRlxYP1iv37Sf4htdNDkaUq9J1WGuoFzWVd2uwiNWk6+HCARvvuesknvbbUfF3X5l9NMk4rIBdRyjlrXw+YDkC3rbulndwxmI4uM4IH2x2FQ0nRn7EEXqDBC5LRKiZtG1NkOO0/4ZjtilAyoI7jadYzaL5ZJgCdPTcagmSMlEEWM9s/gCbzI29NzHgpX1CA== 16, 116 -- X-MS-TrafficTypeDiagnostic: CY4PR02MB3304: 16, 116 -- X-Microsoft-Exchange-Diagnostics: 16, 116 -- 1;CY4PR02MB3304;31:1lOD/F/33n6qmbCrrSvagwV6eUZJo19CIwGzx+lxO13Hi/XE6iwukIa+xlEyTJDenDucoEGDgWNPuahCXMNWQWPu8Jfaf74UMKrlGAR8JtEHeFxDZIvirDPEZTd0rM19P3FnybrhCsMfW71M3u27oHzN1uiajwwOU+DiqRsoaXIILii9lPvRHy5P2pU3yNlYIgwd6lxXkAd/qd158qih3T+LzUkPHXhpn4qDETvLLTU=;20: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 16, 116 -- X-Exchange-Antispam-Report-Test: UriScan:(129945304726314)(180861224563641); 16, 116 -- X-Microsoft-Antispam-PRVS: 16, 116 -- {CY4PR02MB330417DA32D6EF27195A5C4785850-at-CY4PR02MB3304.namprd02.prod.outlook.com} 16, 116 -- X-Exchange-Antispam-Report-CFA-Test: 16, 116 -- BCL:0;PCL:0;RULEID:(100000700101)(100105000095)(100000701101)(100105300095)(100000702101)(100105100095)(6040450)(601004)(2401047)(13013025)(5005006)(8121501046)(13021025)(10201501046)(93006095)(93003095)(100000703101)(100105400095)(3002001)(6041248)(20161123562025)(20161123564025)(201703131423075)(201702281529075)(201702281528075)(201703061421075)(201703061406153)(20161123560025)(20161123555025)(20161123558100)(6072148)(201708071742011)(100000704101)(100105200095)(100000705101)(100105500095);SRVR:CY4PR02MB3304;BCL:0;PCL:0;RULEID:(100000800101)(100110000095)(100000801101)(100110300095)(100000802101)(100110100095)(100000803101)(100110400095)(100000804101)(100110200095)(100000805101)(100110500095);SRVR:CY4PR02MB3304; 16, 116 -- X-Microsoft-Exchange-Diagnostics: 16, 116 -- 1;CY4PR02MB3304;4:uIHOp065pRXXpO+rDsisshk2znEQQoT2+YHP4975zz26LUGJGShiieLGOe8AC9qFNXFI8bKxsWOeS9X/cyVsntdJsbCthWbKPF87DaErEJW0/+9n2BEsnDJ/kOIcbdJsiLclA3T01se914zPX7LrdvR7m7H3zEtdZZvjafjcTcwJ8vhCNjXAOIsNqWDkVwpKhA8hSvwKKqiQCQEnytLlJnJvf66yVL0DyL3RphiUnUF9KlV5DL6gntnoyU3MvTwv4Zo96WfKEVmRfp3CG5JlUK11yEQW+Nr/8jG2CWbSTJs94MZrTX3TRHirZ0meowP2JnQjeO8dC8bu5T2iqg9jKg== 16, 116 -- X-Forefront-PRVS: 040866B734 16, 116 -- X-Microsoft-Exchange-Diagnostics: 16, 116 -- =?us-ascii?Q?1;CY4PR02MB3304;23:mnxXFUnmFfItgRoQKt4cj2Yrpn9zjPe53AjvCAKqL?= 16, 116 -- =?us-ascii?Q?ZQ2003LcNIMPKHyoOJL5TFmzHZJQ5Aat8Rue2maiV09kTYLMlYdS7QHcBHNZ?= 16, 116 -- =?us-ascii?Q?+qBHkRKJHFh1q34O7xCGqfl6QR3RO+42OMGwQ9rKMroPR2fYXy8VmXq+1AJU?= 16, 116 -- =?us-ascii?Q?LxXJawDhlukgX66fYlF3mrjZyTMrnp31Ba1IAvUfCg5JDoBpxPrmhM2aP/np?= 16, 116 -- =?us-ascii?Q?p+R6pIRlWb3gKn/tyS8DTCcKDKBHLv9qVEQo5uWbCaDE/dE6cimcMjNTz5Pj?= 16, 116 -- =?us-ascii?Q?9Y+xPRQJPVtlvyjuX7VwoP6DUFWdGgYN1NvfLlxLmP+q3wK/+ULdIiOEdnBt?= 16, 116 -- =?us-ascii?Q?4dFOqCcqgAZ7pPbaA3dtnYeIUzr3/72dvQUq/p4gOIiinrPnHJrXYf3rvb6u?= 16, 116 -- =?us-ascii?Q?Dr3v5/sWc/HFHXNgkHVYInHNblm1yfLuNx8vYZSYwK82Fd+MeC1uIcMBrmYJ?= 16, 116 -- =?us-ascii?Q?Mc2GJS1RfV5eN7+zmh4IclgylBd0Pz6tDn+zUAXV02j/pIjzPmVd3A6gowOy?= 16, 116 -- =?us-ascii?Q?tCWkhgoIPMYX1VrxaeyOkEjJ11ZGAG5zSO7j39VelWDl+GfrMA/i1qc+ExMz?= 16, 116 -- =?us-ascii?Q?T8LYmrzg+JNTfFSdH2SxQ8q0DrP8Vu+fGvpIMIl6TzaIFJfdJRUQE+eDMIq8?= 16, 116 -- =?us-ascii?Q?QAkf7DumqWh7ReKyy0F4Bo6U59RT8wrN7UQ1s/ZS0kNejkdQuVXftdVdC4Dv?= 16, 116 -- =?us-ascii?Q?bYQ8/JJbdOjcD0hQ13Ho73ljeeoQzdR6BYmTLK/6/q4HxT/qofe/ZcafhAJp?= 16, 116 -- =?us-ascii?Q?dyLMnZSMYqY2o/0w7wiY9vJ05SNyNRF1EuGc6OTgDiizs5yWYE1IGt4UQzwm?= 16, 116 -- =?us-ascii?Q?ffeoymuQeinO7h+cHCVsnlNUY/6Fv4xmCqgLWu22g7PHrSCmTPe7p5PjlN30?= 16, 116 -- =?us-ascii?Q?fAxzAutuzOigp+pwodJSPfxVUC1Z/qnjh1bZ2+rU+vvidEbGWWZrQzgXPiWB?= 16, 116 -- =?us-ascii?Q?JmqcXpe3N7t8DwPnBJHFV/yp3gbkv6lZE+ylAwXygLyrWyyOrFxhdm05itcf?= 16, 116 -- =?us-ascii?Q?PUqfCB+k6Xdpvsc/k4aZVIi8ZB3HDYHQcpOTTmxarbEvI4h+4x96nj8dwlnX?= 16, 116 -- =?us-ascii?Q?baxofS/8xeARMb5XqacMJ3GOqRS6zlJTmkfWDh9zeAZ2a10WblPa3wB8vxSE?= 16, 116 -- =?us-ascii?Q?F31laz+MEuRUeq10z6AcqUFsZOOjuVOjAzIP/r9xuKaD9pUN6+Xeq6qn/oB0?= 16, 116 -- =?us-ascii?Q?4Nd+LBx+qc++HY=3D?= 16, 116 -- X-Microsoft-Exchange-Diagnostics: 16, 116 -- 1;CY4PR02MB3304;6:W+yb2gLY8NSBDhdWwXjXbf1iScwysQ34D4hc+om8DSL2BLRoPjl7jLIkuamxqG9j1ksjlv5FdYkhvxc9CbHwtZJ4c1fbTfQvwTsvOdURU4ew0bfH7x1okStNMNT9twDJNpcGDEK1azVu3zbEYuW0MLJw2BQiyVxP3GL7xwLRRnzFIogK1tiQY15D7PhTDOXtJZNWjRm4lcV20XO0Ei4o6+xAsJMCWHgcmKQFNrpcUnOqbRoXn7SA3pU7NZb3unO5qsZEc87P5+DPRm14waPGy4eGp4yw0LGXFqbdQUb2mo6EsCzD7+IY5q5il02WfV1zcBgBVZvtEEsr/tnEe/QYSA==;5:+phFTpUczHbC+I2UKY0X4QZ89XmZado8XyoXHy7wfm1knIZ7oriKA2pTnuoqzNJ70kvaUh5ckM7hr7AHFUIUJ4Z6t1GAikWTw14al4jO2vTDwQeVp0bNmmm1T6TaLK0HY23OQl9MZwqn3gTXUGWZnw==;24:0aa9uRVl8O/OW1MSq3fSOr/CbFW1ufFV7ypKOMZKAw8yoSxQtO2oo+Ff0Dx3LyGXoijDzs+SSXDi6pNI8pzIqiLZj11mM9FUEBSX/zdRMck=;7:nAMwIAzRdDXOgEvF5dlWJEMAyQKRf5I/eeAYQr17XdzUctUA5yKYQ7yv45oG82pa12PZkB9Tzp1WICY74y4cUFOhTP21mzVG2oN8v9EIlDUgHLkEBvJyOjrRec3mpJMVWMe1kdZ9fXE8718rvXjHqODlnacPtSbkbnlRafrWGLG8PhYW4K3auFYmjX5CZzuhAc2npiWSmxckCyxkGLK4LggcMztiW5weK4blltBwJN0= 16, 116 -- SpamDiagnosticOutput: 1:99 16, 116 -- SpamDiagnosticMetadata: NSPM 16, 116 -- X-OriginatorOrg: magnet.fsu.edu 16, 116 -- X-MS-Exchange-CrossTenant-OriginalArrivalTime: 23 Aug 2017 15:46:43.0862 16, 116 -- (UTC) 16, 116 -- X-MS-Exchange-CrossTenant-Id: a36450eb-db06-42a7-8d1b-026719f701e3 16, 116 -- X-MS-Exchange-CrossTenant-OriginalAttributedTenantConnectingIp: TenantId=a36450eb-db06-42a7-8d1b-026719f701e3;Ip=[146.201.107.249];Helo=[mailrelay04.its.fsu.edu] 16, 116 -- X-MS-Exchange-CrossTenant-FromEntityHeader: HybridOnPrem 16, 116 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: CY4PR02MB3304 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both nikd-at-vsl.cua.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: nikd-at-vsl.cua.edu Name: Nik Deems
Organization: VSL
Title-Subject: [Filtered] Backscatter detector
Message: Might anyone have a lead on a used BSE detector for a JEOL-5910?
Cheers, Nik Deems Electron Microscopy Lab Technician Vitreous State Laboratory
Login Host: 136.242.188.2 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
From kelnanc57-at-gmail.com Thu Aug 24 17:44:29 2017 Return-Path: {kelnanc57-at-gmail.com} Received: from gmail.com ([103.194.186.178]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v7OMiQfs031727 for {microscopylistserverarchive6-at-microscopy.com} ; Thu, 24 Aug 2017 17:44:28 -0500 Message-ID: {D83EB019.A7C4BBC3-at-gmail.com}
X-from: cni-at-udel.edu
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both cni-at-udel.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: cni-at-udel.edu Name: Chaoying Ni
Organization: University of Delaware
Title-Subject: [Filtered] Open position: Research Associate I (28N) - Electron Microscopy
Message: Research Associate I - (Grade 28N) Materials Science and Engineering
Deadline: September 7, 2017
CONTEXT OF THE JOB:
Under limited supervision, performs a variety of complex technical laboratory duties including the design and construction of test equipment. Supervises and trains personnel. The principal emphasis is on working independently and exercising personal initiative in conducting experiments using complex electron and light microscopes, scanning probe microscopes and associated sample preparation equipment and experiment devices.
QUALIFICATIONS:
Requires a minimum of a Bachelor's degree and one year of experience in laboratory work in a relevant field as well as experience with the necessary physical, electronic and chemical systems. Supervisory experience preferred.
Related progressive experience beyond a high school diploma or GED may be substituted for required education or additional related education may be substituted for required experience. Requires knowledge of laboratory techniques, procedures and instrumentation.
Requires abilities to read and interpret complex operations manuals, drawings and design, interpret problems and design equipment for the particular tasks, assume responsibility and take initiative to perform tasks, work independently, train and instruct others, and communicate effectively and interact well with people of all ages and diverse backgrounds.
OCCUPATIONAL EXPOSURES:
May be required to use personal protective equipment to prevent exposure to hazardous materials.
Login Host: 128.4.177.210 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
I recently got a second hand Noran Quest EDX system with a SiLi detector and was wondering if anyone on this list has technical information to share.
The system is currently cooling down with its Dewar filled with LN2. In the "Quest Status" application I get various voltage measurements including a voltage for the LN sensor (currently at 4.33V and slowly decreasing) and a voltage for the detector temperature (currently at 0V). The application also displays a warning "LN error - Bias off".
While I believe that the system has not cooled down sufficiently yet (and hence the bias cannot be turned on without causing damage to the detector), I'm a bit puzzled that the detector temperature voltage is constantly at 0V. I would rather expect a voltage } 0V here that reflects on the current temperature of the detector.
In addition, it would be great to get an idea how those measurement voltages can be transformed into actual temperatures.
From donahenr845uz-at-gmail.com Fri Aug 25 08:15:17 2017 Return-Path: {donahenr845uz-at-gmail.com} Received: from gmail.com ([222.97.39.119]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v7PDFEeH003599 for {microscopylistserverarchive6-at-microscopy.com} ; Fri, 25 Aug 2017 08:15:16 -0500 Received: from unknown (146.249.164.17) by smtp.doneohx.com with LOCAL; Fri, 25 Aug 2017 20:57:06 +0700 Received: from unknown (HELO mx.reskind.net) (Fri, 25 Aug 2017 20:50:19 +0700) by mx03.listsystemsf.net with ESMTP; Fri, 25 Aug 2017 20:50:19 +0700 Received: from unknown (HELO relay-x.misswldrs.com) (Fri, 25 Aug 2017 20:36:29 +0700) by nntp.pinxodet.net with LOCAL; Fri, 25 Aug 2017 20:36:29 +0700 Message-ID: {91583378.77DEF6BA-at-gmail.com}
Dear Colleagues, Plate-like nanoparticles (2-5micron diameter, 20-100nm thick) have been prepared. Plan view was examined by the usual powder dispersion drop cast on a holey-C film method. But, we now would like to look at the cross sections of these particles to study how the atomic layers stack and grow into a plate. The sample comes in water suspension. Two questions: 1, sample prep for cross sections HREM. How do we prepare TEM specimens for high-reso atomic imaging of the cross sections of these thin nano-plates? 2, Good statistics. How can we maximise the number (~30 or more) of the nano-plates in one specimen? Many thanks for your help. Zhou Dr Z Zhou, Loughborough University, UK
==============================Original Headers============================== 2, 80 -- From Z.Zhou-at-lboro.ac.uk Wed Aug 30 09:31:21 2017 2, 80 -- Received: from mta-1.lboro.ac.uk (mta-1.lut.ac.uk [158.125.160.47]) 2, 80 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v7UEVLJF027632 2, 80 -- for {Microscopy-at-microscopy.com} ; Wed, 30 Aug 2017 09:31:21 -0500 2, 80 -- Received: from [158.125.160.17] (helo=itsex-2.lunet.lboro.ac.uk) 2, 80 -- by mta-1.lboro.ac.uk with esmtps (TLSv1.2:ECDHE-RSA-AES256-SHA384:256) 2, 80 -- (Exim 4.89) 2, 80 -- id 1dn51E-0002tk-4n 2, 80 -- for Microscopy-at-microscopy.com; Wed, 30 Aug 2017 16:35:04 +0100 2, 80 -- Received: from itsex-4.lunet.lboro.ac.uk 2, 80 -- (2001:630:301:d314:90c6:812d:223a:24f) by itsex-2.lunet.lboro.ac.uk 2, 80 -- (2001:630:301:d314:580f:144d:cf5e:c130) with Microsoft SMTP Server 2, 80 -- (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_128_CBC_SHA256_P256) id 2, 80 -- 15.1.669.32; Wed, 30 Aug 2017 16:35:03 +0100 2, 80 -- Received: from itsex-1.lunet.lboro.ac.uk 2, 80 -- (2001:630:301:d314:b8fc:8e06:90b0:b933) by itsex-4.lunet.lboro.ac.uk 2, 80 -- (2001:630:301:d314:90c6:812d:223a:24f) with Microsoft SMTP Server 2, 80 -- (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_256_CBC_SHA384_P256) id 2, 80 -- 15.1.845.34; Wed, 30 Aug 2017 16:35:03 +0100 2, 80 -- Received: from EUR03-DB5-obe.outbound.protection.outlook.com (94.245.120.82) 2, 80 -- by itsex-1.lunet.lboro.ac.uk (158.125.160.16) with Microsoft SMTP Server 2, 80 -- (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_256_CBC_SHA384_P384) id 2, 80 -- 15.1.669.32 via Frontend Transport; Wed, 30 Aug 2017 16:35:03 +0100 2, 80 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=lunet.onmicrosoft.com; 2, 80 -- s=selector1-lboro-ac-uk; 2, 80 -- h=From:Date:Subject:Message-ID:Content-Type:MIME-Version; 2, 80 -- bh=LyQsNXXNRMUvonl10W5ic+M/mi1COSAn9BZNAdVACAI=; 2, 80 -- b=g+nWDhMIv6rnU2bkLjZEdgw+bXJika9d4/JrNGnDvFiVXxM1o+iZTPYsPfd+xeyeCNniznm2rLBad8cF5bRk+LGC8C6vhwrlBabrlCa7OM88dD4dHih1jWJ5Vrkv9IubTQYwgsY9ArLU19rkNDo7/EZHDEEe3CgqeiZvG541amo= 2, 80 -- Received: from AM5PR0402MB2771.eurprd04.prod.outlook.com (10.175.41.13) by 2, 80 -- AM5PR0402MB2867.eurprd04.prod.outlook.com (10.175.41.150) with Microsoft SMTP 2, 80 -- Server (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_256_CBC_SHA384_P256) id 2, 80 -- 15.1.1385.9; Wed, 30 Aug 2017 15:35:02 +0000 2, 80 -- Received: from AM5PR0402MB2771.eurprd04.prod.outlook.com 2, 80 -- ([fe80::9dd6:e961:75ed:7ecf]) by AM5PR0402MB2771.eurprd04.prod.outlook.com 2, 80 -- ([fe80::9dd6:e961:75ed:7ecf%13]) with mapi id 15.20.0013.011; Wed, 30 Aug 2, 80 -- 2017 15:35:02 +0000 2, 80 -- From: Zhaoxia Zhou {Z.Zhou-at-lboro.ac.uk} 2, 80 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 2, 80 -- Subject: HRTEM sample prep for cross section imaging of plate-like 2, 80 -- nanoparticles 2, 80 -- Thread-Topic: HRTEM sample prep for cross section imaging of plate-like 2, 80 -- nanoparticles 2, 80 -- Thread-Index: AdMhoWqYlKbPRNWyQGqRbFhw/X1Ung== 2, 80 -- Date: Wed, 30 Aug 2017 15:35:02 +0000 2, 80 -- Message-ID: {AM5PR0402MB2771498696174BD177E8D1DBC69C0-at-AM5PR0402MB2771.eurprd04.prod.outlook.com} 2, 80 -- Accept-Language: en-GB, en-US 2, 80 -- Content-Language: en-US 2, 80 -- X-MS-Has-Attach: 2, 80 -- X-MS-TNEF-Correlator: 2, 80 -- authentication-results: spf=none (sender IP is ) 2, 80 -- smtp.mailfrom=Z.Zhou-at-lboro.ac.uk; 2, 80 -- x-originating-ip: [2001:630:301:3064:94d5:55e3:8a63:d66b] 2, 80 -- x-ms-publictraffictype: Email 2, 80 -- x-microsoft-exchange-diagnostics: 1;AM5PR0402MB2867;6:MvUrxGMU3TsmOYYlM0vEl5Q1kLB3egPn6MDjir4XOVogxdmHirpgJu0hwNMyk6VjTWjWktQ0wy/nrRH62GP265g4Upfr6IBr1TW2yzi/2amb+diyWJeWOHAQmnkUz4ks8aAqcsxeCbeWaMe58nwOIpHk4OYgVItOF/Jbl7+wyYxltsPfIZcZjnc3pI3f1PpHCwEEt9g/JSMPMvqn8yJvKTC9FXW3gyenj8nrpWj3pqY/tz9a414YX14J2wk0H3K0ZyVVYI8dAe+m1Q19yvh6JG2i8Ep83TfvUUGg+oFRX4IgygmAbhqF1KcEasrBfjKhO2SreroCQV9q1fPMl7dMDA==;5:2Aw0VNoSdmo2XUYtXC58G8CZWpspHnC21gKrwCfS3wC/BsLIGWjqPkz+hu9oEpqeG9wxbebo6PXR/v18jFWMvX++8GUjkIORDVuCCDh5vFgO2CRsHVPQYHeQN9nM01pkm6ErhrJolDEfAaTUXbPfAA==;24:XmaV+8863N7tbLY6w0KLPApyDG5AOHpEtVBbuRZgq3BhJw71TXJ0QocqWSQOX60DEYQD7Op7RTk4oU4Un7E4wafHcWwwmjHFnAOoXzbIayc=;7:QfeM+1Rc5fsXY0lzT+qa8Y5XXUgNAJ7KDQ+qqqpw/IMYWjNBaX3B6ejm5JetklzmVh75Zx97Xh5tRa3MMk0YGkd4jSgptR8/WgaYhAQByuLS5wreTlTTybQtf3iF2c2ovyZ752R2NLJZqSOK8pwVhl3hp2PkW1kyPGhB5sTYjZhDb5eqW9RMD9CF2M7FyYpjpCQG9fskZsxxPxXrkcR7B0oVozMLxzT4OTphIBEdNUE= 2, 80 -- x-ms-exchange-antispam-srfa-diagnostics: SSOS; 2, 80 -- x-ms-office365-filtering-correlation-id: 5b2053b0-73e4-4b5d-fb5d-08d4efbcae6a 2, 80 -- x-microsoft-antispam: UriScan:;BCL:0;PCL:0;RULEID:(300000500095)(300135000095)(300000501095)(300135300095)(22001)(300000502095)(300135100095)(2017030254152)(300000503095)(300135400095)(2017052603199)(201703131423075)(201703031133081)(201702281549075)(300000504095)(300135200095)(300000505095)(300135600095)(300000506095)(300135500095);SRVR:AM5PR0402MB2867; 2, 80 -- x-ms-traffictypediagnostic: AM5PR0402MB2867: 2, 80 -- x-exchange-antispam-report-test: UriScan:; 2, 80 -- x-microsoft-antispam-prvs: {AM5PR0402MB2867E8AE15EB1320F4760714C69C0-at-AM5PR0402MB2867.eurprd04.prod.outlook.com} 2, 80 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:(100000700101)(100105000095)(100000701101)(100105300095)(100000702101)(100105100095)(6040450)(601004)(2401047)(8121501046)(5005006)(100000703101)(100105400095)(10201501046)(93006095)(93001095)(3002001)(6041248)(20161123558100)(20161123560025)(201703131423075)(201702281529075)(201702281528075)(201703061421075)(201703061406153)(20161123564025)(20161123562025)(20161123555025)(6072148)(201708071742011)(100000704101)(100105200095)(100000705101)(100105500095);SRVR:AM5PR0402MB2867;BCL:0;PCL:0;RULEID:(100000800101)(100110000095)(100000801101)(100110300095)(100000802101)(100110100095)(100000803101)(100110400095)(100000804101)(100110200095)(100000805101)(100110500095);SRVR:AM5PR0402MB2867; 2, 80 -- x-forefront-prvs: 041517DFAB 2, 80 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10019020)(6009001)(199003)(23363002)(189002)(66654002)(40764003)(8936002)(97736004)(3280700002)(189998001)(110136004)(42882006)(8676002)(81166006)(81156014)(2906002)(6506006)(5660300001)(413944005)(7696004)(2900100001)(3660700001)(6916009)(86362001)(68736007)(72206003)(33656002)(6116002)(102836003)(478600001)(105586002)(14454004)(101416001)(106356001)(50986999)(54356999)(74316002)(5250100002)(55016002)(7736002)(9686003)(99286003)(74482002)(305945005)(6436002)(53936002)(25786009)(491001);DIR:OUT;SFP:1102;SCL:1;SRVR:AM5PR0402MB2867;H:AM5PR0402MB2771.eurprd04.prod.outlook.com;FPR:;SPF:None;PTR:InfoNoRecords;A:1;MX:1;LANG:en; 2, 80 -- received-spf: None (protection.outlook.com: lboro.ac.uk does not designate 2, 80 -- permitted sender hosts) 2, 80 -- spamdiagnosticoutput: 1:99 2, 80 -- spamdiagnosticmetadata: NSPM 2, 80 -- Content-Type: text/plain; charset="utf-8" 2, 80 -- MIME-Version: 1.0 2, 80 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 30 Aug 2017 15:35:02.4594 2, 80 -- (UTC) 2, 80 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted 2, 80 -- X-MS-Exchange-CrossTenant-id: cf264fc0-aeb8-449f-9054-82ce4454084b 2, 80 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: AM5PR0402MB2867 2, 80 -- X-OriginatorOrg: lboro.ac.uk 2, 80 -- X-Scan-Signature: 5a36bda3904d05fd053fd54b64d98d6d 2, 80 -- X-Lboro-Creds: scanned on mta-1.lboro.ac.uk 2, 80 -- X-Lboro-Filtered: mta-1.lboro.ac.uk, Wed, 30 Aug 2017 16:35:04 +0100 2, 80 -- Content-Transfer-Encoding: 8bit 2, 80 -- X-MIME-Autoconverted: from base64 to 8bit by microscopy.com id v7UEVLJF027632 ==============================End of - Headers==============================
What are these plates like - brittle or ductile? Electrically conductive? Magnetic? Hard? What are they like to ion mill?
My instinct says embed them in epoxy, squash it as it sets so they want to line up perpendicular to the compression, then turn the glued block round 90 degrees, take a section, and mechanically thin and ion mill it. But if they are resistant to ion milling the block might just fall apart as the epoxy mills away instead, or you will get thin epoxy and thick useless bits of plates. If you encapsulate them in some soft metal and do a similar thing they won't be aligned so well and if they are brittle they will break up and end up mostly the wrong way.
Looking at the size, you could *almost* pick them up one by one with a micromanipulator, stick them to something parallel, and FIB the whole stack, given that you are meticulous and persistent. But this may be just a fancy way of having a terrible week at work.
Jo
On 30 August 2017 at 15:47, {Z.Zhou-at-lboro.ac.uk} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Colleagues, } Plate-like nanoparticles (2-5micron diameter, 20-100nm thick) have been prepared. Plan view was examined by the usual powder dispersion drop cast on a holey-C film method. But, we now would like to look at the cross sections of these particles to study how the atomic layers stack and grow into a plate. The sample comes in water suspension. } Two questions: } 1, sample prep for cross sections HREM. How do we prepare TEM specimens for high-reso atomic imaging of the cross sections of these thin nano-plates? } 2, Good statistics. How can we maximise the number (~30 or more) of the nano-plates in one specimen? } Many thanks for your help. } Zhou } Dr Z Zhou, Loughborough University, UK } } } ==============================Original Headers============================== } 2, 80 -- From Z.Zhou-at-lboro.ac.uk Wed Aug 30 09:31:21 2017 } 2, 80 -- Received: from mta-1.lboro.ac.uk (mta-1.lut.ac.uk [158.125.160.47]) } 2, 80 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v7UEVLJF027632 } 2, 80 -- for {Microscopy-at-microscopy.com} ; Wed, 30 Aug 2017 09:31:21 -0500 } 2, 80 -- Received: from [158.125.160.17] (helo=itsex-2.lunet.lboro.ac.uk) } 2, 80 -- by mta-1.lboro.ac.uk with esmtps (TLSv1.2:ECDHE-RSA-AES256-SHA384:256) } 2, 80 -- (Exim 4.89) } 2, 80 -- id 1dn51E-0002tk-4n } 2, 80 -- for Microscopy-at-microscopy.com; Wed, 30 Aug 2017 16:35:04 +0100 } 2, 80 -- Received: from itsex-4.lunet.lboro.ac.uk } 2, 80 -- (2001:630:301:d314:90c6:812d:223a:24f) by itsex-2.lunet.lboro.ac.uk } 2, 80 -- (2001:630:301:d314:580f:144d:cf5e:c130) with Microsoft SMTP Server } 2, 80 -- (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_128_CBC_SHA256_P256) id } 2, 80 -- 15.1.669.32; Wed, 30 Aug 2017 16:35:03 +0100 } 2, 80 -- Received: from itsex-1.lunet.lboro.ac.uk } 2, 80 -- (2001:630:301:d314:b8fc:8e06:90b0:b933) by itsex-4.lunet.lboro.ac.uk } 2, 80 -- (2001:630:301:d314:90c6:812d:223a:24f) with Microsoft SMTP Server } 2, 80 -- (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_256_CBC_SHA384_P256) id } 2, 80 -- 15.1.845.34; Wed, 30 Aug 2017 16:35:03 +0100 } 2, 80 -- Received: from EUR03-DB5-obe.outbound.protection.outlook.com (94.245.120.82) } 2, 80 -- by itsex-1.lunet.lboro.ac.uk (158.125.160.16) with Microsoft SMTP Server } 2, 80 -- (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_256_CBC_SHA384_P384) id } 2, 80 -- 15.1.669.32 via Frontend Transport; Wed, 30 Aug 2017 16:35:03 +0100 } 2, 80 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=lunet.onmicrosoft.com; } 2, 80 -- s=selector1-lboro-ac-uk; } 2, 80 -- h=From:Date:Subject:Message-ID:Content-Type:MIME-Version; } 2, 80 -- bh=LyQsNXXNRMUvonl10W5ic+M/mi1COSAn9BZNAdVACAI=; } 2, 80 -- b=g+nWDhMIv6rnU2bkLjZEdgw+bXJika9d4/JrNGnDvFiVXxM1o+iZTPYsPfd+xeyeCNniznm2rLBad8cF5bRk+LGC8C6vhwrlBabrlCa7OM88dD4dHih1jWJ5Vrkv9IubTQYwgsY9ArLU19rkNDo7/EZHDEEe3CgqeiZvG541amo= } 2, 80 -- Received: from AM5PR0402MB2771.eurprd04.prod.outlook.com (10.175.41.13) by } 2, 80 -- AM5PR0402MB2867.eurprd04.prod.outlook.com (10.175.41.150) with Microsoft SMTP } 2, 80 -- Server (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_256_CBC_SHA384_P256) id } 2, 80 -- 15.1.1385.9; Wed, 30 Aug 2017 15:35:02 +0000 } 2, 80 -- Received: from AM5PR0402MB2771.eurprd04.prod.outlook.com } 2, 80 -- ([fe80::9dd6:e961:75ed:7ecf]) by AM5PR0402MB2771.eurprd04.prod.outlook.com } 2, 80 -- ([fe80::9dd6:e961:75ed:7ecf%13]) with mapi id 15.20.0013.011; Wed, 30 Aug } 2, 80 -- 2017 15:35:02 +0000 } 2, 80 -- From: Zhaoxia Zhou {Z.Zhou-at-lboro.ac.uk} } 2, 80 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} } 2, 80 -- Subject: HRTEM sample prep for cross section imaging of plate-like } 2, 80 -- nanoparticles } 2, 80 -- Thread-Topic: HRTEM sample prep for cross section imaging of plate-like } 2, 80 -- nanoparticles } 2, 80 -- Thread-Index: AdMhoWqYlKbPRNWyQGqRbFhw/X1Ung== } 2, 80 -- Date: Wed, 30 Aug 2017 15:35:02 +0000 } 2, 80 -- Message-ID: {AM5PR0402MB2771498696174BD177E8D1DBC69C0-at-AM5PR0402MB2771.eurprd04.prod.outlook.com} } 2, 80 -- Accept-Language: en-GB, en-US } 2, 80 -- Content-Language: en-US } 2, 80 -- X-MS-Has-Attach: } 2, 80 -- X-MS-TNEF-Correlator: } 2, 80 -- authentication-results: spf=none (sender IP is ) } 2, 80 -- smtp.mailfrom=Z.Zhou-at-lboro.ac.uk; } 2, 80 -- x-originating-ip: [2001:630:301:3064:94d5:55e3:8a63:d66b] } 2, 80 -- x-ms-publictraffictype: Email } 2, 80 -- x-microsoft-exchange-diagnostics: 1;AM5PR0402MB2867;6:MvUrxGMU3TsmOYYlM0vEl5Q1kLB3egPn6MDjir4XOVogxdmHirpgJu0hwNMyk6VjTWjWktQ0wy/nrRH62GP265g4Upfr6IBr1TW2yzi/2amb+diyWJeWOHAQmnkUz4ks8aAqcsxeCbeWaMe58nwOIpHk4OYgVItOF/Jbl7+wyYxltsPfIZcZjnc3pI3f1PpHCwEEt9g/JSMPMvqn8yJvKTC9FXW3gyenj8nrpWj3pqY/tz9a414YX14J2wk0H3K0ZyVVYI8dAe+m1Q19yvh6JG2i8Ep83TfvUUGg+oFRX4IgygmAbhqF1KcEasrBfjKhO2SreroCQV9q1fPMl7dMDA==;5:2Aw0VNoSdmo2XUYtXC58G8CZWpspHnC21gKrwCfS3wC/BsLIGWjqPkz+hu9oEpqeG9wxbebo6PXR/v18jFWMvX++8GUjkIORDVuCCDh5vFgO2CRsHVPQYHeQN9nM01pkm6ErhrJolDEfAaTUXbPfAA==;24:XmaV+8863N7tbLY6w0KLPApyDG5AOHpEtVBbuRZgq3BhJw71TXJ0QocqWSQOX60DEYQD7Op7RTk4oU4Un7E4wafHcWwwmjHFnAOoXzbIayc=;7:QfeM+1Rc5fsXY0lzT+qa8Y5XXUgNAJ7KDQ+qqqpw/IMYWjNBaX3B6ejm5JetklzmVh75Zx97Xh5tRa3MMk0YGkd4jSgptR8/WgaYhAQByuLS5wreTlTTybQtf3iF2c2ovyZ752R2NLJZqSOK8pwVhl3hp2PkW1kyPGhB5sTYjZhDb5eqW9RMD9CF2M7FyYpjpCQG9fskZsxxPxXrkcR7B0oVozMLxzT4OTphIBEdNUE= } 2, 80 -- x-ms-exchange-antispam-srfa-diagnostics: SSOS; } 2, 80 -- x-ms-office365-filtering-correlation-id: 5b2053b0-73e4-4b5d-fb5d-08d4efbcae6a } 2, 80 -- x-microsoft-antispam: UriScan:;BCL:0;PCL:0;RULEID:(300000500095)(300135000095)(300000501095)(300135300095)(22001)(300000502095)(300135100095)(2017030254152)(300000503095)(300135400095)(2017052603199)(201703131423075)(201703031133081)(201702281549075)(300000504095)(300135200095)(300000505095)(300135600095)(300000506095)(300135500095);SRVR:AM5PR0402MB2867; } 2, 80 -- x-ms-traffictypediagnostic: AM5PR0402MB2867: } 2, 80 -- x-exchange-antispam-report-test: UriScan:; } 2, 80 -- x-microsoft-antispam-prvs: {AM5PR0402MB2867E8AE15EB1320F4760714C69C0-at-AM5PR0402MB2867.eurprd04.prod.outlook.com} } 2, 80 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:(100000700101)(100105000095)(100000701101)(100105300095)(100000702101)(100105100095)(6040450)(601004)(2401047)(8121501046)(5005006)(100000703101)(100105400095)(10201501046)(93006095)(93001095)(3002001)(6041248)(20161123558100)(20161123560025)(201703131423075)(201702281529075)(201702281528075)(201703061421075)(201703061406153)(20161123564025)(20161123562025)(20161123555025)(6072148)(201708071742011)(100000704101)(100105200095)(100000705101)(100105500095);SRVR:AM5PR0402MB2867;BCL:0;PCL:0;RULEID:(100000800101)(100110000095)(100000801101)(100110300095)(100000802101)(100110100095)(100000803101)(100110400095)(100000804101)(100110200095)(100000805101)(100110500095);SRVR:AM5PR0402MB2867; } 2, 80 -- x-forefront-prvs: 041517DFAB } 2, 80 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10019020)(6009001)(199003)(23363002)(189002)(66654002)(40764003)(8936002)(97736004)(3280700002)(189998001)(110136004)(42882006)(8676002)(81166006)(81156014)(2906002)(6506006)(5660300001)(413944005)(7696004)(2900100001)(3660700001)(6916009)(86362001)(68736007)(72206003)(33656002)(6116002)(102836003)(478600001)(105586002)(14454004)(101416001)(106356001)(50986999)(54356999)(74316002)(5250100002)(55016002)(7736002)(9686003)(99286003)(74482002)(305945005)(6436002)(53936002)(25786009)(491001);DIR:OUT;SFP:1102;SCL:1;SRVR:AM5PR0402MB2867;H:AM5PR0402MB2771.eurprd04.prod.outlook.com;FPR:;SPF:None;PTR:InfoNoRecords;A:1;MX:1;LANG:en; } 2, 80 -- received-spf: None (protection.outlook.com: lboro.ac.uk does not designate } 2, 80 -- permitted sender hosts) } 2, 80 -- spamdiagnosticoutput: 1:99 } 2, 80 -- spamdiagnosticmetadata: NSPM } 2, 80 -- Content-Type: text/plain; charset="utf-8" } 2, 80 -- MIME-Version: 1.0 } 2, 80 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 30 Aug 2017 15:35:02.4594 } 2, 80 -- (UTC) } 2, 80 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted } 2, 80 -- X-MS-Exchange-CrossTenant-id: cf264fc0-aeb8-449f-9054-82ce4454084b } 2, 80 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: AM5PR0402MB2867 } 2, 80 -- X-OriginatorOrg: lboro.ac.uk } 2, 80 -- X-Scan-Signature: 5a36bda3904d05fd053fd54b64d98d6d } 2, 80 -- X-Lboro-Creds: scanned on mta-1.lboro.ac.uk } 2, 80 -- X-Lboro-Filtered: mta-1.lboro.ac.uk, Wed, 30 Aug 2017 16:35:04 +0100 } 2, 80 -- Content-Transfer-Encoding: 8bit } 2, 80 -- X-MIME-Autoconverted: from base64 to 8bit by microscopy.com id v7UEVLJF027632 } ==============================End of - Headers==============================
Jo mentioned something similar to this already, but Schreiber et al. have a nice paper showing correlated plan view and x-section imaging of nanostructures via FIB preparation. The reference is here:
This probably won't satisfy your requirement of having tens of platelets in a single cross section sample, but I suppose you could deposit a high concentration on a support film and hunt around until you find a area with many stacked like shingles. Or as Jo mentioned, this all may be a great way to punish a graduate student!
Good luck, Chris
On Wed, Aug 30, 2017 at 10:43 AM, {Z.Zhou-at-lboro.ac.uk} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Colleagues, } Plate-like nanoparticles (2-5micron diameter, 20-100nm thick) have been prepared. Plan view was examined by the usual powder dispersion drop cast on a holey-C film method. But, we now would like to look at the cross sections of these particles to study how the atomic layers stack and grow into a plate. The sample comes in water suspension. } Two questions: } 1, sample prep for cross sections HREM. How do we prepare TEM specimens for high-reso atomic imaging of the cross sections of these thin nano-plates? } 2, Good statistics. How can we maximise the number (~30 or more) of the nano-plates in one specimen? } Many thanks for your help. } Zhou } Dr Z Zhou, Loughborough University, UK } } } ==============================Original Headers============================== } 2, 80 -- From Z.Zhou-at-lboro.ac.uk Wed Aug 30 09:31:21 2017 } 2, 80 -- Received: from mta-1.lboro.ac.uk (mta-1.lut.ac.uk [158.125.160.47]) } 2, 80 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v7UEVLJF027632 } 2, 80 -- for {Microscopy-at-microscopy.com} ; Wed, 30 Aug 2017 09:31:21 -0500 } 2, 80 -- Received: from [158.125.160.17] (helo=itsex-2.lunet.lboro.ac.uk) } 2, 80 -- by mta-1.lboro.ac.uk with esmtps (TLSv1.2:ECDHE-RSA-AES256-SHA384:256) } 2, 80 -- (Exim 4.89) } 2, 80 -- id 1dn51E-0002tk-4n } 2, 80 -- for Microscopy-at-microscopy.com; Wed, 30 Aug 2017 16:35:04 +0100 } 2, 80 -- Received: from itsex-4.lunet.lboro.ac.uk } 2, 80 -- (2001:630:301:d314:90c6:812d:223a:24f) by itsex-2.lunet.lboro.ac.uk } 2, 80 -- (2001:630:301:d314:580f:144d:cf5e:c130) with Microsoft SMTP Server } 2, 80 -- (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_128_CBC_SHA256_P256) id } 2, 80 -- 15.1.669.32; Wed, 30 Aug 2017 16:35:03 +0100 } 2, 80 -- Received: from itsex-1.lunet.lboro.ac.uk } 2, 80 -- (2001:630:301:d314:b8fc:8e06:90b0:b933) by itsex-4.lunet.lboro.ac.uk } 2, 80 -- (2001:630:301:d314:90c6:812d:223a:24f) with Microsoft SMTP Server } 2, 80 -- (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_256_CBC_SHA384_P256) id } 2, 80 -- 15.1.845.34; Wed, 30 Aug 2017 16:35:03 +0100 } 2, 80 -- Received: from EUR03-DB5-obe.outbound.protection.outlook.com (94.245.120.82) } 2, 80 -- by itsex-1.lunet.lboro.ac.uk (158.125.160.16) with Microsoft SMTP Server } 2, 80 -- (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_256_CBC_SHA384_P384) id } 2, 80 -- 15.1.669.32 via Frontend Transport; Wed, 30 Aug 2017 16:35:03 +0100 } 2, 80 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=lunet.onmicrosoft.com; } 2, 80 -- s=selector1-lboro-ac-uk; } 2, 80 -- h=From:Date:Subject:Message-ID:Content-Type:MIME-Version; } 2, 80 -- bh=LyQsNXXNRMUvonl10W5ic+M/mi1COSAn9BZNAdVACAI=; } 2, 80 -- b=g+nWDhMIv6rnU2bkLjZEdgw+bXJika9d4/JrNGnDvFiVXxM1o+iZTPYsPfd+xeyeCNniznm2rLBad8cF5bRk+LGC8C6vhwrlBabrlCa7OM88dD4dHih1jWJ5Vrkv9IubTQYwgsY9ArLU19rkNDo7/EZHDEEe3CgqeiZvG541amo= } 2, 80 -- Received: from AM5PR0402MB2771.eurprd04.prod.outlook.com (10.175.41.13) by } 2, 80 -- AM5PR0402MB2867.eurprd04.prod.outlook.com (10.175.41.150) with Microsoft SMTP } 2, 80 -- Server (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_256_CBC_SHA384_P256) id } 2, 80 -- 15.1.1385.9; Wed, 30 Aug 2017 15:35:02 +0000 } 2, 80 -- Received: from AM5PR0402MB2771.eurprd04.prod.outlook.com } 2, 80 -- ([fe80::9dd6:e961:75ed:7ecf]) by AM5PR0402MB2771.eurprd04.prod.outlook.com } 2, 80 -- ([fe80::9dd6:e961:75ed:7ecf%13]) with mapi id 15.20.0013.011; Wed, 30 Aug } 2, 80 -- 2017 15:35:02 +0000 } 2, 80 -- From: Zhaoxia Zhou {Z.Zhou-at-lboro.ac.uk} } 2, 80 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} } 2, 80 -- Subject: HRTEM sample prep for cross section imaging of plate-like } 2, 80 -- nanoparticles } 2, 80 -- Thread-Topic: HRTEM sample prep for cross section imaging of plate-like } 2, 80 -- nanoparticles } 2, 80 -- Thread-Index: AdMhoWqYlKbPRNWyQGqRbFhw/X1Ung== } 2, 80 -- Date: Wed, 30 Aug 2017 15:35:02 +0000 } 2, 80 -- Message-ID: {AM5PR0402MB2771498696174BD177E8D1DBC69C0-at-AM5PR0402MB2771.eurprd04.prod.outlook.com} } 2, 80 -- Accept-Language: en-GB, en-US } 2, 80 -- Content-Language: en-US } 2, 80 -- X-MS-Has-Attach: } 2, 80 -- X-MS-TNEF-Correlator: } 2, 80 -- authentication-results: spf=none (sender IP is ) } 2, 80 -- smtp.mailfrom=Z.Zhou-at-lboro.ac.uk; } 2, 80 -- x-originating-ip: [2001:630:301:3064:94d5:55e3:8a63:d66b] } 2, 80 -- x-ms-publictraffictype: Email } 2, 80 -- x-microsoft-exchange-diagnostics: 1;AM5PR0402MB2867;6:MvUrxGMU3TsmOYYlM0vEl5Q1kLB3egPn6MDjir4XOVogxdmHirpgJu0hwNMyk6VjTWjWktQ0wy/nrRH62GP265g4Upfr6IBr1TW2yzi/2amb+diyWJeWOHAQmnkUz4ks8aAqcsxeCbeWaMe58nwOIpHk4OYgVItOF/Jbl7+wyYxltsPfIZcZjnc3pI3f1PpHCwEEt9g/JSMPMvqn8yJvKTC9FXW3gyenj8nrpWj3pqY/tz9a414YX14J2wk0H3K0ZyVVYI8dAe+m1Q19yvh6JG2i8Ep83TfvUUGg+oFRX4IgygmAbhqF1KcEasrBfjKhO2SreroCQV9q1fPMl7dMDA==;5:2Aw0VNoSdmo2XUYtXC58G8CZWpspHnC21gKrwCfS3wC/BsLIGWjqPkz+hu9oEpqeG9wxbebo6PXR/v18jFWMvX++8GUjkIORDVuCCDh5vFgO2CRsHVPQYHeQN9nM01pkm6ErhrJolDEfAaTUXbPfAA==;24:XmaV+8863N7tbLY6w0KLPApyDG5AOHpEtVBbuRZgq3BhJw71TXJ0QocqWSQOX60DEYQD7Op7RTk4oU4Un7E4wafHcWwwmjHFnAOoXzbIayc=;7:QfeM+1Rc5fsXY0lzT+qa8Y5XXUgNAJ7KDQ+qqqpw/IMYWjNBaX3B6ejm5JetklzmVh75Zx97Xh5tRa3MMk0YGkd4jSgptR8/WgaYhAQByuLS5wreTlTTybQtf3iF2c2ovyZ752R2NLJZqSOK8pwVhl3hp2PkW1kyPGhB5sTYjZhDb5eqW9RMD9CF2M7FyYpjpCQG9fskZsxxPxXrkcR7B0oVozMLxzT4OTphIBEdNUE= } 2, 80 -- x-ms-exchange-antispam-srfa-diagnostics: SSOS; } 2, 80 -- x-ms-office365-filtering-correlation-id: 5b2053b0-73e4-4b5d-fb5d-08d4efbcae6a } 2, 80 -- x-microsoft-antispam: UriScan:;BCL:0;PCL:0;RULEID:(300000500095)(300135000095)(300000501095)(300135300095)(22001)(300000502095)(300135100095)(2017030254152)(300000503095)(300135400095)(2017052603199)(201703131423075)(201703031133081)(201702281549075)(300000504095)(300135200095)(300000505095)(300135600095)(300000506095)(300135500095);SRVR:AM5PR0402MB2867; } 2, 80 -- x-ms-traffictypediagnostic: AM5PR0402MB2867: } 2, 80 -- x-exchange-antispam-report-test: UriScan:; } 2, 80 -- x-microsoft-antispam-prvs: {AM5PR0402MB2867E8AE15EB1320F4760714C69C0-at-AM5PR0402MB2867.eurprd04.prod.outlook.com} } 2, 80 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:(100000700101)(100105000095)(100000701101)(100105300095)(100000702101)(100105100095)(6040450)(601004)(2401047)(8121501046)(5005006)(100000703101)(100105400095)(10201501046)(93006095)(93001095)(3002001)(6041248)(20161123558100)(20161123560025)(201703131423075)(201702281529075)(201702281528075)(201703061421075)(201703061406153)(20161123564025)(20161123562025)(20161123555025)(6072148)(201708071742011)(100000704101)(100105200095)(100000705101)(100105500095);SRVR:AM5PR0402MB2867;BCL:0;PCL:0;RULEID:(100000800101)(100110000095)(100000801101)(100110300095)(100000802101)(100110100095)(100000803101)(100110400095)(100000804101)(100110200095)(100000805101)(100110500095);SRVR:AM5PR0402MB2867; } 2, 80 -- x-forefront-prvs: 041517DFAB } 2, 80 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10019020)(6009001)(199003)(23363002)(189002)(66654002)(40764003)(8936002)(97736004)(3280700002)(189998001)(110136004)(42882006)(8676002)(81166006)(81156014)(2906002)(6506006)(5660300001)(413944005)(7696004)(2900100001)(3660700001)(6916009)(86362001)(68736007)(72206003)(33656002)(6116002)(102836003)(478600001)(105586002)(14454004)(101416001)(106356001)(50986999)(54356999)(74316002)(5250100002)(55016002)(7736002)(9686003)(99286003)(74482002)(305945005)(6436002)(53936002)(25786009)(491001);DIR:OUT;SFP:1102;SCL:1;SRVR:AM5PR0402MB2867;H:AM5PR0402MB2771.eurprd04.prod.outlook.com;FPR:;SPF:None;PTR:InfoNoRecords;A:1;MX:1;LANG:en; } 2, 80 -- received-spf: None (protection.outlook.com: lboro.ac.uk does not designate } 2, 80 -- permitted sender hosts) } 2, 80 -- spamdiagnosticoutput: 1:99 } 2, 80 -- spamdiagnosticmetadata: NSPM } 2, 80 -- Content-Type: text/plain; charset="utf-8" } 2, 80 -- MIME-Version: 1.0 } 2, 80 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 30 Aug 2017 15:35:02.4594 } 2, 80 -- (UTC) } 2, 80 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted } 2, 80 -- X-MS-Exchange-CrossTenant-id: cf264fc0-aeb8-449f-9054-82ce4454084b } 2, 80 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: AM5PR0402MB2867 } 2, 80 -- X-OriginatorOrg: lboro.ac.uk } 2, 80 -- X-Scan-Signature: 5a36bda3904d05fd053fd54b64d98d6d } 2, 80 -- X-Lboro-Creds: scanned on mta-1.lboro.ac.uk } 2, 80 -- X-Lboro-Filtered: mta-1.lboro.ac.uk, Wed, 30 Aug 2017 16:35:04 +0100 } 2, 80 -- Content-Transfer-Encoding: 8bit } 2, 80 -- X-MIME-Autoconverted: from base64 to 8bit by microscopy.com id v7UEVLJF027632 } ==============================End of - Headers==============================
==============================Original Headers============================== 7, 44 -- From microwink-at-gmail.com Wed Aug 30 11:29:34 2017 7, 44 -- Received: from mail-wm0-f65.google.com (mail-wm0-f65.google.com [74.125.82.65]) 7, 44 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v7UGTYWn015159 7, 44 -- for {Microscopy-at-microscopy.com} ; Wed, 30 Aug 2017 11:29:34 -0500 7, 44 -- Received: by mail-wm0-f65.google.com with SMTP id i76so2492906wme.3 7, 44 -- for {Microscopy-at-microscopy.com} ; Wed, 30 Aug 2017 10:33:52 -0700 (PDT) 7, 44 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 7, 44 -- d=gmail.com; s=20161025; 7, 44 -- h=mime-version:in-reply-to:references:from:date:message-id:subject:to 7, 44 -- :content-transfer-encoding; 7, 44 -- bh=GHEEOyKtHVanFIlRORIWWjJmF2RODqZOAZc9Odi6Fo0=; 7, 44 -- b=MPNk4dgR+ME7sWk0Gut8K3IIINbgG9Qd1kZ4tQ9Ti0I9XVyrE5F5I8W5aqNG1ibecf 7, 44 -- hfLyiuWJ4ZHLfpBWSpJNe9i3qgxqBv8J+FBt7ttMat4lvDDikEnXxO44YOYz8X1S2/4L 7, 44 -- YYylf8LsnefvkLzUty+9KDWChMe/3e5XbO3B5jX/FfS8phW3cWD5GNYwAasNLmZNDOzn 7, 44 -- m7xKv5SywIsG1G71C1jD9vw7y8xTb87Ncp7Uk4xq/a9eQg/jmgyon+dhMQPP3HtOjntT 7, 44 -- rzqLAg6lGi/T9UOYFytvSb1xcSc8YZ7UOngoZYSvD+pgExJg/aIcd8E0xQNRCtvfNGZ4 7, 44 -- qpJQ== 7, 44 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 7, 44 -- d=1e100.net; s=20161025; 7, 44 -- h=x-gm-message-state:mime-version:in-reply-to:references:from:date 7, 44 -- :message-id:subject:to:content-transfer-encoding; 7, 44 -- bh=GHEEOyKtHVanFIlRORIWWjJmF2RODqZOAZc9Odi6Fo0=; 7, 44 -- b=T9vwgPQIZMtyOYMbGwxzXrkuJUN3lvZIVhl95oXgB3d2PBLWj2/3YDeq1LwGenFyfx 7, 44 -- oLDlFfLnUHaQ+MgiGqd9vvwHayCKq8NO5AeZIzSjnWbpx4br6aiMf0deYd8IuapBcGWl 7, 44 -- Dte60LNILzQtREi2jgjqKjNTgzvXu+1p92anBocWMdPvEyvLe/prpFi6BfXwkzSUFoDi 7, 44 -- aZSTp6NSUgu2BVCw2X2rI6LioY4caG8Z+K63mS6nM8PagFsv7IZgGI7n97Wu7tX4NGRu 7, 44 -- knDOKW23ohcI2Fdm/qsWdCgs2gYdBiC1/1tUEl0bYybCzn1jGJ5PGDgiHVfXhcagCnRq 7, 44 -- L+Lg== 7, 44 -- X-Gm-Message-State: AHYfb5ju0szAwG442xirldPUeeJdtErr8r5tpYJygSz6hR8Kg6khh1hq 7, 44 -- E7eC0xCpfBiY6+KwSST8Zrf3k5nYlRtyK0I= 7, 44 -- X-Received: by 10.28.211.77 with SMTP id k74mr1597719wmg.93.1504114431143; 7, 44 -- Wed, 30 Aug 2017 10:33:51 -0700 (PDT) 7, 44 -- MIME-Version: 1.0 7, 44 -- Received: by 10.28.71.221 with HTTP; Wed, 30 Aug 2017 10:33:50 -0700 (PDT) 7, 44 -- In-Reply-To: {201708301443.v7UEhNep005127-at-microscopy.com} 7, 44 -- References: {201708301443.v7UEhNep005127-at-microscopy.com} 7, 44 -- From: Christopher Winkler {microwink-at-gmail.com} 7, 44 -- Date: Wed, 30 Aug 2017 13:33:50 -0400 7, 44 -- Message-ID: {CAA8T2PMmh4WJy1ny3Wth4xS6xzYhz392r8M_ub5g7qtKbtQj7w-at-mail.gmail.com} 7, 44 -- Subject: Re: [Microscopy] HRTEM sample prep for cross section imaging of plate-like 7, 44 -- To: Microscopy-at-microscopy.com 7, 44 -- Content-Type: text/plain; charset="UTF-8" 7, 44 -- Content-Transfer-Encoding: 8bit 7, 44 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v7UGTYWn015159 ==============================End of - Headers==============================
From annepenn4-at-gmail.com Wed Aug 30 13:37:01 2017 Return-Path: {annepenn4-at-gmail.com} Received: from gmail.com ([220.125.146.235]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v7UIawB8016105 for {microscopylistserverarchive6-at-microscopy.com} ; Wed, 30 Aug 2017 13:37:00 -0500 Message-ID: {5B87926A.F755785F-at-gmail.com}
Hello everyone,
We’re in the process of installing a new aberration-corrected STEM and we’re trying to diagnose some noise in the 100-400 Hz range. In particular, our room has a rather high ceiling and we’re concerned about issues due to the room volume.
I’ve seen that some other laboratories use sound-dampening drapes and I’m wondering if these are common for rooms with high ceilings. Fire code issues make it difficult for us to set up these drapes, so it would help to know what experiences others have had.
Does anyone have any suggestions? Thanks!
______________________________________ Steven R. Spurgeon, Ph.D. Postdoctoral Research Associate Physical and Computational Sciences Directorate
Pacific Northwest National Laboratory 902 Battelle Boulevard P.O. Box 999 MSIN:K8-87 Richland, WA 99352
We faced a similar problem trying to look at the cross sectional structure of hard nanowire heterostructures (See Jiang et al Nanoletters, 13, 5135 2013 and Zheng et al. nanoletters 13, 3742 2013) In the end we borrowed a page from our biological colleagues and embedded and microtomed them! Surprisingly successful, you will have to do this very carefully at liq nitrogen temperatures with a diamond knife. If you want the details of microtoming hard materials look up the papers by Selwyn Glanville. The biggest struggle you will have is getting your plates to line up and disperse for the embedding (easy with epitaxially grown samples). This will also give you MANY plates in x-section, so plenty of statistics.
Regards
Matthew
} Dear Colleagues, } Plate-like nanoparticles (2-5micron diameter, 20-100nm thick) have } been prepared. Plan view was examined by the usual powder dispersion } drop cast on a holey-C film method. But, we now would like to look at } the cross sections of these particles to study how the atomic layers } stack and grow into a plate. The sample comes in water suspension. } Two questions: } 1, sample prep for cross sections HREM. How do we prepare TEM } specimens for high-reso atomic imaging of the cross sections of these } thin nano-plates? } 2, Good statistics. How can we maximise the number (~30 or more) of } the nano-plates in one specimen? } Many thanks for your help. } Zhou } Dr Z Zhou, Loughborough University, UK
--
*Dr Matthew Weyland * Associate Professor
*Monash Centre for Electron Microscopy and Department of Materials Science and Engineering* 10 Innovation Walk, Clayton Campus Monash University Clayton VIC 3800 Australia
I thought it would be helpful to summarize the emails I received. The responses were mixed, but most people said that curtains will not help in the 100-400 Hz frequency range.
There were a few suggestions that the source might be the air diffusers or somewhere else in the ventilation system, so we will look into this in more detail. As far as solutions, some people suggested acoustic traps and various insulation options, but it seems that this frequency range is difficult to block out.
We’ll keep hunting!
______________________________________ Steven R. Spurgeon, Ph.D. Postdoctoral Research Associate Physical and Computational Sciences Directorate
Pacific Northwest National Laboratory 902 Battelle Boulevard P.O. Box 999 MSIN:K8-87 Richland, WA 99352
On 8/30/17, 3:45 PM, "Spurgeon, Steven R" {steven.spurgeon-at-pnnl.gov} wrote:
Hello everyone,
We’re in the process of installing a new aberration-corrected STEM and we’re trying to diagnose some noise in the 100-400 Hz range. In particular, our room has a rather high ceiling and we’re concerned about issues due to the room volume.
I’ve seen that some other laboratories use sound-dampening drapes and I’m wondering if these are common for rooms with high ceilings. Fire code issues make it difficult for us to set up these drapes, so it would help to know what experiences others have had.
Does anyone have any suggestions? Thanks!
______________________________________ Steven R. Spurgeon, Ph.D. Postdoctoral Research Associate Physical and Computational Sciences Directorate
Pacific Northwest National Laboratory 902 Battelle Boulevard P.O. Box 999 MSIN:K8-87 Richland, WA 99352
we are going to buy a routine 120 kV TEM, mainly as a plastic sections, negative staining etc. workhorse. Nevertheless, one part of the tender will be also a cryoholder for the very preliminary screening of the SPA grids and a little bit of CEMOVIS. As I relatively understand to most of the microscope parameters (there are more or less 3 potential guys - Jeol 1400+, FEI Talos 120 kV and Hitachi HT7800), I really don’t know how to choose the camera. It should be bottom-mounted and more sensitive than the basic cameras. I am fully aware that we cannot afford a direct detector by far (we don’t even reach the other CCD cameras from Gatan…), but - could somebody tell me what features and parameters I should follow, which numbers are describing what? There is actually almost no materials, leaflets and brochures about the camera of the above-mentioned producers (or their third-party suppliers). Please advise, even the tips for the actual camera type.
Best regards
Adam Schrofel Faculty of Nature, Charles University, Prague
==============================Original Headers============================== 4, 48 -- From adam.schrofel-at-gmail.com Thu Aug 31 11:01:02 2017 4, 48 -- Received: from mail-wm0-f44.google.com (mail-wm0-f44.google.com [74.125.82.44]) 4, 48 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v7VG12C4004924 4, 48 -- for {Microscopy-at-microscopy.com} ; Thu, 31 Aug 2017 11:01:02 -0500 4, 48 -- Received: by mail-wm0-f44.google.com with SMTP id r202so573900wmd.0 4, 48 -- for {Microscopy-at-microscopy.com} ; Thu, 31 Aug 2017 10:05:23 -0700 (PDT) 4, 48 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 48 -- d=gmail.com; s=20161025; 4, 48 -- h=from:content-transfer-encoding:mime-version:subject:message-id:date 4, 48 -- :to; 4, 48 -- bh=+yS0ryFMGldUHOslI2fOvEWSutYAUSAz2sjp0MowG6k=; 4, 48 -- b=szmiM+X+WhRQArHaKJkWLuHHbsP4oPDKceSxnoJFeyRWWbNyRPxpg4IAgg39yrswTh 4, 48 -- 1zGCAEEJmQcYfxT30jDpIughrEXK5WjrmePmt9MjIV4mXgaxnKyF+KPUtjet3s8mFWyD 4, 48 -- DwOJvQtOpWjs6uzUH4xLENzO8Ujmm9ZEilave9WOqvJUB/a96QkYj/Caa/7DXKPa4KNU 4, 48 -- QF4JSJRQ03c0lnrYkQGyBkOfoNFiVuSm4b2daLFIEmx1NYulRiBH9b8PJEUdQwKRj+a1 4, 48 -- IWHO/ZZh/6mLLmLY0pc1hP2fvnMrjl6Yn5tcKrZuUKb7u6tZMuCWx3bRML7StQLEykC5 4, 48 -- eoqA== 4, 48 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 48 -- d=1e100.net; s=20161025; 4, 48 -- h=x-gm-message-state:from:content-transfer-encoding:mime-version 4, 48 -- :subject:message-id:date:to; 4, 48 -- bh=+yS0ryFMGldUHOslI2fOvEWSutYAUSAz2sjp0MowG6k=; 4, 48 -- b=RXUmdVljW/kS4P6JLeGeTfLY1net21YCwD9NO+CiMGagb5wRrfnhBplMNXQa78kxfa 4, 48 -- KHH4TEdwClzdAdLzxYTsEbB12jNl6GMQccpGO4rGCzncTMZ7w73EM8Uwj54o0QWoJEI6 4, 48 -- ri2ES0Y4nJyxlW7yRANCl1zDuEEC9kfDJe+ykL5vJbQ3ZVEsjZcRW8s0unjIBD+PtrwP 4, 48 -- AUQ2BbIsjyiy+L/bfm9VgEtGYYIxDnN2J2yEn7rC3m6iJ7h5yBPPo4lZkabNGPXdUIWx 4, 48 -- IusbA69pVb09aI6oJ9M/jevSNmlUUwB3smWB7mfJptUPY/jznTsijPvHpc/Wb/XOtnDj 4, 48 -- vOyg== 4, 48 -- X-Gm-Message-State: AHYfb5gw9+MLCffbok3l+d5iqrVavXd0LcD5MkF80z0PaDiA/sHmviNN 4, 48 -- 40MV2uHErRNlP5SKvGE= 4, 48 -- X-Google-Smtp-Source: ADKCNb4wW0i+6u29HfIG6ivvOZdebl8/6f6dBN9oJaLXq4ELos2u8rV5M9A8yPADefkxyi3KCpl1Rw== 4, 48 -- X-Received: by 10.80.181.78 with SMTP id z14mr497627edd.105.1504199123149; 4, 48 -- Thu, 31 Aug 2017 10:05:23 -0700 (PDT) 4, 48 -- Received: from [10.2.101.177] (cbg-alt-client.mpi-cbg.de. [141.5.11.6]) 4, 48 -- by smtp.gmail.com with ESMTPSA id w13sm265596edb.16.2017.08.31.10.05.22 4, 48 -- for {Microscopy-at-microscopy.com} 4, 48 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 4, 48 -- Thu, 31 Aug 2017 10:05:22 -0700 (PDT) 4, 48 -- From: =?utf-8?Q?Adam_Schr=C3=B6fel?= {adam.schrofel-at-gmail.com} 4, 48 -- Content-Type: text/plain; charset=utf-8 4, 48 -- Mime-Version: 1.0 (Mac OS X Mail 10.3 \(3273\)) 4, 48 -- Subject: Biology TEM camera 4, 48 -- Message-Id: {03E59EB4-6C97-4A9B-A72B-BC64A1AF1238-at-gmail.com} 4, 48 -- Date: Thu, 31 Aug 2017 19:05:24 +0200 4, 48 -- To: Microscopy-at-microscopy.com 4, 48 -- X-Mailer: Apple Mail (2.3273) 4, 48 -- Content-Transfer-Encoding: 8bit 4, 48 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v7VG12C4004924 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both wagneran-at-uwec.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: wagneran-at-uwec.edu Name: Tony Wagner
Organization: University of Wisconsin - Eau Claire
Title-Subject: [Filtered] Desktop Carbon Coater
Message: Hello Everyone
We are looking to upgrade our carbon coater from an old Edwards Evaporator using rods to a carbon filament in a desktop unit. I have no experience with the carbon fiber systems and am looking for feedback from anyone who has used a carbon fiber based carbon coater in a desktop unit. I am currently looking at the the Denton Desk V HP with tilt and rotation. Have you been happy with the conductive coatings produced and if not what have the issues been.
Thank you in advance for you response.
Login Host: 137.28.229.141 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both zhiqiang-at-pdx.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: zhiqiang-at-pdx.edu Name: Zhiqiang Chen
Organization: Center for Electron Microscopy and Nanofabrication
Title-Subject: [Filtered] Job opening: Research Analyst 3 (TEM)
Message: Center for Electron Microscopy and Nanofabrication at Portland State University has an open position of Research Analyst 3 (TEM). Online application is available at https://jobs.hrc.pdx.edu/postings/24142. Thank you for your attention.
Login Host: 131.252.125.44 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both tamarps-at-technion.ac.il as well as the Microscopy Listserver ---------------------------------------------------------------------------
Organization: Technion- Israel Institute of Technology
Title-Subject: [Filtered] Postdoctoral position Message: Postdoc Position in Functional Nanoparticles and Emulsion Imaging
A postdoctoral position is available in a fascinating project on understanding the formation, dynamics, and properties of new-type emulsions and bijels. The researcher will use the state-of-the-art cryo-SEM and cryo-TEM facilities in the Electron Microscopy Center for Soft Matter in the Department of Chemical Engineering, Technion, to study the relations between functionalized nanoparticles and emulsion and bijels structure and behavior. The postdoc will be part of a collaboration between the Technion-Israel Institute of Technology (Asst. Prof. Tamar Segal-Peretzs group and Asst. Prof. Ofer Manors group) and the University of Calgary, Canada (Asst. Prof. Milana Trifkovics group and Prof. Steven L. Bryants group). The postdoc will work mainly at the Technion, Haifa, Israel, with travel opportunities to Calgary.
Applicants should have a strong background in electron microscopy, preferably with cryo-imaging experience. Additional background in colloidal science is an advantage. Ability to work in a team, as well as excellent verbal and written communication skills are expected.
To apply, please send a cover letter, curriculum vitae, and 1-2 relevant published papers to the e-mail below. The title of the email should read: Postdoctoral positionץ
Contact: Tamar Segal-Peretz Department of Chemical Engineering Technion - Israel Institute of Technology Email: tamarps-at-technion.ac.il Website: http://fnai.technion.ac.il/
Login Host: 79.180.12.171 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
I need to compensate magnetic stray field influences on high working distances in a field emission SEM. On my old SEM I had three pairs of Helmholtz coils but now I intend to go to three single X / Y / Z room-installed coils.
Without compensation the actual stray fields in a distance of ca. one meter are ca. 20 nano-Tesla, near the specimen position on the outside chamber wall ca. 30 nano-Tesla, with Z being a little higher than X and Y.
What I read is that room-installed coils will produce a more uniform compensation field and Helmholtz coils might deal better with stronger stray field influence.
I am grateful for any hints before deciding...
Best,
Stefan
-- ----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
==============================Original Headers============================== 12, 23 -- From stefan.diller-at-t-online.de Sun Sep 3 10:17:07 2017 12, 23 -- Received: from mailout08.t-online.de (mailout08.t-online.de [194.25.134.20]) 12, 23 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v83FH7sK007666 12, 23 -- for {microscopy-at-microscopy.com} ; Sun, 3 Sep 2017 10:17:07 -0500 12, 23 -- Received: from fwd16.aul.t-online.de (fwd16.aul.t-online.de [172.20.26.243]) 12, 23 -- by mailout08.t-online.de (Postfix) with SMTP id 33DDA41C59D9 12, 23 -- for {microscopy-at-microscopy.com} ; Sun, 3 Sep 2017 18:21:39 +0200 (CEST) 12, 23 -- Received: from [192.168.2.115] (rX-nxrZlQhSAz-Tmm4U2bPCKyHzjXrb-VsbV4wpsGNJjhObm4ALMFTaxp-HxLkfgMM-at-[80.130.162.153]) by fwd16.t-online.de 12, 23 -- with (TLSv1.2:ECDHE-RSA-AES256-GCM-SHA384 encrypted) 12, 23 -- esmtp id 1doXeT-4Kr9iS0; Sun, 3 Sep 2017 18:21:37 +0200 12, 23 -- To: microscopy-at-microscopy.com 12, 23 -- From: Stefan Diller {stefan.diller-at-t-online.de} 12, 23 -- Subject: Helmholtz coils versus room coil installation to compensate stray 12, 23 -- fields 12, 23 -- Message-ID: {4e69fec8-11f2-9efc-9e3b-b0cf9ed64536-at-t-online.de} 12, 23 -- Date: Sun, 3 Sep 2017 18:21:29 +0200 12, 23 -- User-Agent: Mozilla/5.0 (Windows NT 10.0; WOW64; rv:52.0) Gecko/20100101 12, 23 -- Thunderbird/52.3.0 12, 23 -- MIME-Version: 1.0 12, 23 -- Content-Type: text/plain; charset=utf-8; format=flowed 12, 23 -- Content-Transfer-Encoding: 7bit 12, 23 -- X-ID: rX-nxrZlQhSAz-Tmm4U2bPCKyHzjXrb-VsbV4wpsGNJjhObm4ALMFTaxp-HxLkfgMM 12, 23 -- X-TOI-MSGID: 9b5b363c-a259-4a3e-9bf4-701286b40a85 ==============================End of - Headers==============================
From smithejoseph18k-at-gmail.com Mon Sep 4 22:47:20 2017 Return-Path: {smithejoseph18k-at-gmail.com} Received: from gmail.com (mail.dongnamdc.com [222.97.39.100] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v853lHfh013091 for {microscopylistserverarchive6-at-microscopy.com} ; Mon, 4 Sep 2017 22:47:19 -0500 Received: from relay-x.misswldrs.com [200.213.235.156] by qrx.quickslick.com with QMQP; Mon, 04 Sep 2017 20:32:32 -0700 Received: from smtp4.cyberemailings.com ([Mon, 04 Sep 2017 20:14:24 -0700]) by asx121.turbo-inline.com with SMTP; Mon, 04 Sep 2017 20:14:24 -0700 Message-ID: {AD49D893.491F620E-at-gmail.com}
Dear List,
We're buying a new TEM, and it is hard to come to a conclusion as to what sort of vacuum pump combination would be best for us.
We do 120kV biological TEM of resin-impregnated sections, no cryo work. Our specimens are beam stable, and we've not been using a cold finger anti-contamination design on our previous TEMs going back 30 years; we plan to continue without one. We vent the specimen airlock with air from the room- should we move to nitrogen? We will exclusively use LaB6 filaments.
If you could choose an ideal vacuum system combination to achieve the following ordered priorities, what pumps would it consist of?
1) Reliability- low maintenance, long lifetime 2) Speed- fast pump down from specimen changes, and from filament changes. 3) Clean- probably more for the cooled (-20 C) camera than anything else, but also to extend filament life and reduce contamination of specimen 4) Good ultimate vacuum- for increased lifetime of LaB6 filament 5) Quiet
My main questions are: Is an Ion Getter pump needed, if we aren't doing cryo, so there will be comparatively little water vapour pumping? Is there much difference in our application for turbo molecular pumps that are oil-lubricated vs magnetic levitation bearings? Should we really be using a cold finger? Should we be venting the specimen airlock with dry nitrogen?
Possible system 1: Rotary pump, 2x oil diffusion pumps
Possible system 2: Scroll pump, turbo molecular pump
Possible system 3: Diaphragm pump, turbo molecular pump, large IGP on column, small IGP on gun.
Best regards,
Ben
-- Research Support Manager (Imaging and IT Systems) MRC Brain Network Dynamics Unit, University of Oxford Department of Pharmacology, Mansfield Road, Oxford, OX1 3TH, United Kingdom. Telephone: 01865 271897 {http://www.mrcbndu.ox.ac.uk/}
==============================Original Headers============================== 13, 29 -- From ben.micklem-at-pharm.ox.ac.uk Tue Sep 5 04:32:28 2017 13, 29 -- Received: from relay12.mail.ox.ac.uk (relay12.mail.ox.ac.uk [129.67.1.163]) 13, 29 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v859WR5h022884 13, 29 -- for {microscopy-at-microscopy.com} ; Tue, 5 Sep 2017 04:32:28 -0500 13, 29 -- Received: from hub05.nexus.ox.ac.uk ([163.1.154.231] helo=HUB05.ad.oak.ox.ac.uk) 13, 29 -- by relay12.mail.ox.ac.uk with esmtp (Exim 4.89) 13, 29 -- (envelope-from {ben.micklem-at-pharm.ox.ac.uk} ) 13, 29 -- id 1dpBE9-0002ny-fD 13, 29 -- for microscopy-at-microscopy.com; Tue, 05 Sep 2017 11:37:06 +0100 13, 29 -- Received: from MBX03.ad.oak.ox.ac.uk ([169.254.3.69]) by HUB05.ad.oak.ox.ac.uk 13, 29 -- ([163.1.154.96]) with mapi id 14.03.0248.002; Tue, 5 Sep 2017 11:36:40 +0100 13, 29 -- From: Ben Micklem {ben.micklem-at-pharm.ox.ac.uk} 13, 29 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 13, 29 -- Subject: vacuum for bio TEM non-cryo 120kV 13, 29 -- Thread-Topic: vacuum for bio TEM non-cryo 120kV 13, 29 -- Thread-Index: AdMmMFqeQcECTM6BSFWbSk5Jr8oBaw== 13, 29 -- Date: Tue, 5 Sep 2017 10:36:39 +0000 13, 29 -- Message-ID: {168A63C1140FEB4EB61870A513446A4A18A54FA5-at-MBX03.ad.oak.ox.ac.uk} 13, 29 -- Accept-Language: en-GB, en-US 13, 29 -- Content-Language: en-US 13, 29 -- X-MS-Has-Attach: 13, 29 -- X-MS-TNEF-Correlator: 13, 29 -- x-originating-ip: [172.16.150.240] 13, 29 -- Content-Type: text/plain; charset="us-ascii" 13, 29 -- MIME-Version: 1.0 13, 29 -- X-Oxmail-Spam-Status: score=0.0 tests=none 13, 29 -- X-Oxmail-Spam-Level: / 13, 29 -- Content-Transfer-Encoding: 8bit 13, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v859WR5h022884 ==============================End of - Headers==============================
I've worked with Zeiss Raw Materials to organize the first general short course on microscopy and spectroscopy for the 2017 Geological Society of America Annual Meeting in Seattle. Short courses run right before the meeting and are offered to both attendees and the public.
I'd love for you to join us there to look at imaging and characterizing rocks with photons, electrons, ions, and x-rays.
High Resolution and Correlative Microscopy and Spectroscopy for the Geosciences
Saturday, October 21, 2017, from 8 AM to 5 PM
Washington State Convention Center, Meeting Room 212
The cost is $100 if you sign up by September 18th and you're registered for the meeting, plus $40 for non-registrants and $30 after early registration. The course is worth 0.8 CEUs, and lunch and coffee breaks are provided.
Instructors: K. Leo Pullin, Consultant; Matthew Andrew, Carl Zeiss X-Ray Microscopy; Sreenivas Bhattiprolu, Carl Zeiss X-Ray Microscopy.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Jen.Fendler-at-utas.edu.au as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: Jen.Fendler-at-utas.edu.au Name: Jen Fendler
Organization: University of Tasmania
Title-Subject: [Filtered] air table for ultramicrotome
Message: Dear TEM experts
Are there any cons to purchasing an air table rather than a passive antivibration table for an ultramicrotome?
Our granite table is inadequate most days (although it was built pseudo in-house rather than commercial purpose built for ultramicrotome, and was sufficient before major hospital renovations nearby), and our situation is that our TEM facility is on the 5th floor, and we have numerous major ongoing building works on the blocks adjacent to our building, so I doubt even a move to the basement would help for the next 1-2 years.
I wondered why air tables are not commonly used for ultramicrotomes and if there's any rationale against this, and would like to know before purchasing one. I'm trialing one at the moment and it works well (other than minor annoyance of how easily it is bumped by the slightest brush of eyes on oculars etc)
Kind regards Jen
Login Host: 131.217.255.26 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
} On Sep 5, 2017, at 2:49 AM, ben.micklem-at-pharm.ox.ac.uk wrote: } } } Dear List, } } We're buying a new TEM, and it is hard to come to a conclusion as to what sort of vacuum pump combination would be best for us. } } We do 120kV biological TEM of resin-impregnated sections, no cryo work. Our specimens are beam stable, and we've not been using a cold finger anti-contamination design on our previous TEMs going back 30 years; we plan to continue without one. We vent the specimen airlock with air from the room- should we move to nitrogen? We will exclusively use LaB6 filaments. } } If you could choose an ideal vacuum system combination to achieve the following ordered priorities, what pumps would it consist of? } } 1) Reliability- low maintenance, long lifetime } 2) Speed- fast pump down from specimen changes, and from filament changes. } 3) Clean- probably more for the cooled (-20 C) camera than anything else, but also to extend filament life and reduce contamination of specimen } 4) Good ultimate vacuum- for increased lifetime of LaB6 filament } 5) Quiet } } My main questions are: } Is an Ion Getter pump needed, if we aren't doing cryo, so there will be comparatively little water vapour pumping? } Is there much difference in our application for turbo molecular pumps that are oil-lubricated vs magnetic levitation bearings? } Should we really be using a cold finger? } Should we be venting the specimen airlock with dry nitrogen? } } Possible system 1: } Rotary pump, 2x oil diffusion pumps } } Possible system 2: } Scroll pump, turbo molecular pump } } Possible system 3: } Diaphragm pump, turbo molecular pump, large IGP on column, small IGP on gun. } } Best regards, } } Ben } } -- } Research Support Manager (Imaging and IT Systems) } MRC Brain Network Dynamics Unit, } University of Oxford Department of Pharmacology, } Mansfield Road, Oxford, OX1 3TH, United Kingdom. } Telephone: 01865 271897 {http://www.mrcbndu.ox.ac.uk/} } Dear Ben, We had a Techni 12 for scanning grids and doing routine work when I managed the facility at Caltech. The scope was the most reliable and trouble-free I’ve worked with. I must also add the disclaimer that I worked for about a year for FEI. The vacuum system that came with the scope was excellent, and it included ion pumps. I definitely recommend a cold finger. When the beam hits a resin specimen, organic fragments are produced that a cold finger will trap. This will prevent the column from getting contaminated and give you longer trouble-free service. I do not think you need to vent the airlock with nitrogen, but I was working in a place where the air was usually very dry. If Oxford is humid, dry nitrogen may be an advantage. Since the airlock has a small volume, a nitrogen tank should last a very long time and not be very expensive. Yours, Bill
==============================Original Headers============================== 6, 33 -- From wtivol-at-sbcglobal.net Tue Sep 5 18:09:47 2017 6, 33 -- Received: from nm13-vm4.access.bullet.mail.bf1.yahoo.com (nm13-vm4.access.bullet.mail.bf1.yahoo.com [216.109.115.4]) 6, 33 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v85N9lu4011448 6, 33 -- for {microscopy-at-microscopy.com} ; Tue, 5 Sep 2017 18:09:47 -0500 6, 33 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=sbcglobal.net; s=s2048; t=1504656868; bh=cNKsXgYL822cGGoRvTxTkP8eB1Sphv5cVZE3vs6c4cQ=; h=Subject:From:In-Reply-To:Date:References:To:From:Subject; b=Ah5V52okDMJucTx3lHwiv+g9XketzAzhJasyxQHGqWHUsfT60prBe+OhIUCmeDpOGvKnVw1p83gwA4agb8LPXDNO1D9xlTnkANm73YPI/wmTDNgxmgAmLhO7/EPLEQjbrAeMlhENPS4igdUEGirv6VaB7EtkPctlGgkiJeobc/sJPQt4Ujm1TKjVjN8dgsSsksSA2uudPnV2dajRkw1tbvmcfvKcmnpvSkbdnGBTSYHycqOazgaPo9jRK9kw/vjTzgYk6wm0BcAyYKOi6leOkhyqL8WV4QwqW4cc/eIYNibIrGeFaJEBUfC/8JhJbevwmuBg4reCvaM+2DsYnobTVg== 6, 33 -- Received: from [66.196.81.156] by nm13.access.bullet.mail.bf1.yahoo.com with NNFMP; 06 Sep 2017 00:14:28 -0000 6, 33 -- Received: from [98.138.226.243] by tm2.access.bullet.mail.bf1.yahoo.com with NNFMP; 06 Sep 2017 00:14:28 -0000 6, 33 -- Received: from [127.0.0.1] by smtp114.sbc.mail.ne1.yahoo.com with NNFMP; 06 Sep 2017 00:14:27 -0000 6, 33 -- X-Yahoo-Newman-Id: 975092.64643.bm-at-smtp114.sbc.mail.ne1.yahoo.com 6, 33 -- X-Yahoo-Newman-Property: ymail-3 6, 33 -- X-YMail-OSG: Cuit1ksVM1n6..FZSrwuPgzYCNb9RR3l5DB.4RWmG9mc_Ez 6, 33 -- Lf6vfDcEZWtrg2_Y4jJI97Rbzo0O4CobXU4NlZqdgODeCTjdxWFOn1MoI_Y4 6, 33 -- KVhM2jiuVpFF.NGSFq8rOEIc6.sFppBuVV9.d.UcPDQkfvsBKDT5JW6HHNnT 6, 33 -- tqin5Ku68INGQQKJJXK2.WlG_hbvN8_SgxaF9hCD5rTsqKpWu3jBTGq35cVG 6, 33 -- 627kjR42pfbnTzfkHvWQ9Coi8w0xybWSWKHsvgskAd1lqA1xnjob6UyAbHto 6, 33 -- 629sNCL0ZoW_KhmQiRBsndIl6yKgF42UZK3hr6tvCLeAlJ6xR.b632G8czzp 6, 33 -- Pv2nqRENHJxbL5R_VrasiVlltL8CHZg6TAqRnfM3_GtPcFZVfohmAvkFESIF 6, 33 -- 0U.9TerByCd.QOX_4P7e5BRzwhdjS90xkkZS_BBfKKH58yoprQ.IsYQ2kCEy 6, 33 -- iBM0P62gYmrmI1075xMMtCxnD5bsBUam6KQMMA8F4d6JkKdbv_cnLy7RAD0Z 6, 33 -- F6KSYjTt_EfY- 6, 33 -- X-Yahoo-SMTP: 2C4lFAKswBB2zWDtHIVLYPvHWQTcLYoM2wWnLTebSN_t 6, 33 -- Content-Type: text/plain; charset=utf-8 6, 33 -- Mime-Version: 1.0 (Mac OS X Mail 9.3 \(3124\)) 6, 33 -- Subject: Re: [Microscopy] vacuum for bio TEM non-cryo 120kV 6, 33 -- From: Bill & Sue Tivol {wtivol-at-sbcglobal.net} 6, 33 -- In-Reply-To: {201709050949.v859nlWP004770-at-microscopy.com} 6, 33 -- Date: Tue, 5 Sep 2017 17:14:25 -0700 6, 33 -- Message-Id: {BA23C344-E47F-4D74-BDE0-F9D08F5A15D8-at-sbcglobal.net} 6, 33 -- References: {201709050949.v859nlWP004770-at-microscopy.com} 6, 33 -- To: ben.micklem-at-pharm.ox.ac.uk, microscopy-at-microscopy.com 6, 33 -- X-Mailer: Apple Mail (2.3124) 6, 33 -- Content-Transfer-Encoding: 8bit 6, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v85N9lu4011448 ==============================End of - Headers==============================
Please join us for the first “International Workshop and Short Course on the Frontiers of Electron Tomography in the Physical Sciences” (www.electron-tomo.com) at Berkeley, CA, USA, on Oct. 23 - 26, 2017.This four-day event will consist of a two-day scientific workshop on advanced electron tomography with invited and contributed talks followed by a two-day short course on nanometer to atomic-resolution electron tomography for graduate and postdoc level scientists.
--- Registration deadline is September 15th, 2017 ---
Please visit the conference website to register for the workshop, submit a contributed talk/poster or request to attend the short course. Space is especially limited for the short course so please register as soon as possible if you are interested to attend.
The two-day workshop will bring together leading figures in electron tomography and related fields to disseminate results and exchange ideas. Topics to be covered include:
- Atomic Electron Tomography (AET) - Electron Tomography at the Nanoscale - Electron Holography - Scanning Confocal Electron Microscopy - Other 3D Imaging / Diffraction Methods - Applications in the Physical Sciences
We expect about 100 participants to attend this workshop, which will consist of invited talks, contributed talks and posters.
The subsequent Short Course is designed to teach the basic theory, implementation and application of electron tomography for nanometer- and atomic-resolution structural analysis. We will cover:
- Electron tomography theory - Tilt-series pre-processing - Basic and advanced reconstruction algorithms - Three-dimensional data analysis and visualization
The goal of the short course is to train graduate students and postdocs to be the future leaders in the field of high-resolution electron tomography and to pursue its broad applications across different disciplines.
Best regards,
Peter Ercius, Mary Scott, Colin Ophus, & John Miao
Members of the Organizing Committee of FET 2017
==============================Original Headers============================== 13, 47 -- From percius-at-lbl.gov Thu Sep 7 14:01:52 2017 13, 47 -- Received: from fe4.lbl.gov (fe4.lbl.gov [128.3.41.71]) 13, 47 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v87J1pVK027017 13, 47 -- for {microscopy-at-microscopy.com} ; Thu, 7 Sep 2017 14:01:51 -0500 13, 47 -- X-Ironport-SBRS: 2.7 13, 47 -- X-IronPort-Anti-Spam-Filtered: true 13, 47 -- X-IronPort-Anti-Spam-Result: =?us-ascii?q?A2GlCgByprFZf8bZVdFcHgYMGQYMgwICU?= 13, 47 -- =?us-ascii?q?D9uJweDcJpChAVaAZVLKYERiAwHQhUBAQEBAQEBAQEBAQIQAQEJCwsIJjGCMyS?= 13, 47 -- =?us-ascii?q?BK1tlRTcPAiYCJBIBBQGIRIIiBZ0og0VAjAuCJ4ttEnuCHYICgU6EWoR/c4JHg?= 13, 47 -- =?us-ascii?q?mEFkjeOPYFtkmSCE4VnineRSYNOGR+BFTWBMCgKAh8IIg9jBoFGDxeCPAEBCAE?= 13, 47 -- =?us-ascii?q?BAQGCZSA2h2iCQQEBAQ?= 13, 47 -- X-IronPort-AV: E=Sophos;i="5.42,360,1500966000"; 13, 47 -- d="scan'208";a="87639819" 13, 47 -- Received: from mail-ua0-f198.google.com ([209.85.217.198]) 13, 47 -- by fe4.lbl.gov with ESMTP; 07 Sep 2017 13:06:24 -0700 13, 47 -- Received: by mail-ua0-f198.google.com with SMTP id k23so355680uaf.0 13, 47 -- for {microscopy-at-microscopy.com} ; Thu, 07 Sep 2017 13:06:24 -0700 (PDT) 13, 47 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 13, 47 -- d=1e100.net; s=20161025; 13, 47 -- h=x-gm-message-state:mime-version:from:date:message-id:subject:to 13, 47 -- :content-transfer-encoding; 13, 47 -- bh=RVgUD3bIxi1Oqm8kjIKTO4WrCqVHyk4n7GbTCcZCUS8=; 13, 47 -- b=YoHIzbsSYNuPBoCaa1AIxU3ul65wQVJO6UiTqcp1QlLRxO4+MeDcETQDjcxyZO76bj 13, 47 -- pMZDMDEvwbGLPn9bvebrfPa/Zh2eOWSP3MEr7i5hYIoxgdx0iVVhy14S94yT8DVu42bC 13, 47 -- Cbq11uvODXgnaqKGHIRf58/RsS1dSqT/wP6QM37uBUNo/qOoqDas2xLV4oGtGagKWtZ7 13, 47 -- b+4U8vnWVfUsk10O2ojCMCG+aPdKzXIV5wxDCKdK9RJ9CuaTInVaW7ZWWn9C1Og46LA4 13, 47 -- O/No6ZwPOF8bxgAW6IpVP9tsOU47hHI6B0fgQ/Wlv3tM5k/3WM3vgm4RZZpPFgxr82yg 13, 47 -- uWcA== 13, 47 -- X-Gm-Message-State: AHPjjUh/BvtBgHFYR58dmJJXRle0WrLGBCn9SpneUDSmINmge7wt06iz 13, 47 -- gXg5ul83JlAr7Uu/QfNRJyppQXXkzio0AXsYuzp4piRdj5G5dzQuUcM/t/U72Waq95iPm+mebGz 13, 47 -- aBWjF5g9FWxrudvrhki8lT6qAwMq5 13, 47 -- X-Received: by 10.31.153.4 with SMTP id b4mr355743vke.46.1504814783726; 13, 47 -- Thu, 07 Sep 2017 13:06:23 -0700 (PDT) 13, 47 -- X-Google-Smtp-Source: AOwi7QBndXSl+UbkKAi4fyO+kPVc4XzD7XnotFnoTcwhcNljuqHApLQ4SxJbrHc6Q4/a9E+Q59iU80O/ZkcW7PFOc8w= 13, 47 -- X-Received: by 10.31.153.4 with SMTP id b4mr355735vke.46.1504814783438; Thu, 13, 47 -- 07 Sep 2017 13:06:23 -0700 (PDT) 13, 47 -- MIME-Version: 1.0 13, 47 -- Received: by 10.159.48.27 with HTTP; Thu, 7 Sep 2017 13:06:03 -0700 (PDT) 13, 47 -- From: Peter Ercius {percius-at-lbl.gov} 13, 47 -- Date: Thu, 7 Sep 2017 13:06:03 -0700 13, 47 -- Message-ID: {CAHdm6ZsZjgpLhJ=r=yjT-wmsqDYkF9HWgYD8u2iJqAv5RRpjsw-at-mail.gmail.com} 13, 47 -- Subject: Frontiers of Electron Tomography Workshop and Short Course in 13, 47 -- Berkeley, CA, USA 13, 47 -- To: microscopy-at-microscopy.com 13, 47 -- Content-Type: text/plain; charset="UTF-8" 13, 47 -- Content-Transfer-Encoding: 8bit 13, 47 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v87J1pVK027017 ==============================End of - Headers==============================
The New England Society for Microscopy invites you to join us at JEOL in Peabody, MA for our annual fall meeting on Sept 28th.
To learn more about our speakers and to register, please visit our website at http://nesmicroscopy.org/upcoming-meetings/.
We hope to see you there!
Cheers, -Blanca 2017 NESM President
----------------------------------------- Blanca Carbajal Gonzalez, M.S. Director of Microscopy Science Center 50 College St Mount Holyoke College Clapp Laboratory 123 Office: 413-538-3118 Cell: 559-905-7138 bicarbaj-at-mtholyoke.edu MHC Microscopy
X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu}
Jen,
Do you have partially-deflated (old) tennis balls, raquet balls, or the like between the base of your granite table and the top? That should help at least with the vibration.
If not … maybe a magician who knows how to levitate things? Or an air table.
Phil ------------- Philip Oshel Imaging Facility Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 989 774-3576 office 989 774-7567 lab
Email: Jen.Fendler-at-utas.edu.au Name: Jen Fendler
Organization: University of Tasmania
Title-Subject: [Filtered] air table for ultramicrotome
Message: Dear TEM experts
Are there any cons to purchasing an air table rather than a passive antivibration table for an ultramicrotome?
Our granite table is inadequate most days (although it was built pseudo in-house rather than commercial purpose built for ultramicrotome, and was sufficient before major hospital renovations nearby), and our situation is that our TEM facility is on the 5th floor, and we have numerous major ongoing building works on the blocks adjacent to our building, so I doubt even a move to the basement would help for the next 1-2 years.
I wondered why air tables are not commonly used for ultramicrotomes and if there's any rationale against this, and would like to know before purchasing one. I'm trialing one at the moment and it works well (other than minor annoyance of how easily it is bumped by the slightest brush of eyes on oculars etc)
What in the world is your TEM doing on the 5th floor? How do you handle vibration for the TEM? A. John Mardinly, Ph.D., P.E. Retired, ASU
} } Message: Dear TEM experts } } Are there any cons to purchasing an air table rather than a passive } antivibration table for an ultramicrotome? } } Our granite table is inadequate most days (although it was built pseudo } in-house rather than commercial purpose built for ultramicrotome, and } was sufficient before major hospital renovations nearby), and our } situation is that our TEM facility is on the 5th floor, and we have } numerous major ongoing building works on the blocks adjacent to our } building, so I doubt even a move to the basement would help for the next } 1-2 years. } } I wondered why air tables are not commonly used for ultramicrotomes and } if there's any rationale against this, and would like to know before } purchasing one. I'm trialing one at the moment and it works well (other } than minor annoyance of how easily it is bumped by the slightest brush } of eyes on oculars etc) } } Kind regards } Jen } } } } ==============================Original Headers============================== } 19, 53 -- From microscopy.listserver-at-gmail.com Sun Sep 10 23:41:01 2017 } 19, 53 -- Received: from mail-wm0-f48.google.com (mail-wm0-f48.google.com [74.125.82.48]) } 19, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v8B4f1es000891 } 19, 53 -- for {microscopy-at-microscopy.com} ; Sun, 10 Sep 2017 23:41:01 -0500 } 19, 53 -- Received: by mail-wm0-f48.google.com with SMTP id f199so30991322wme.0 } 19, 53 -- for {microscopy-at-microscopy.com} ; Sun, 10 Sep 2017 22:46:01 -0700 (PDT) } 19, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 19, 53 -- d=gmail.com; s=20161025; } 19, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 19, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 19, 53 -- bh=mMISr17c45+HxKA6htDQWDJH0Z+vg7iKrjYy2l1RHNE=; } 19, 53 -- b=GRJ9ww8fEGv7ghF2TmxPBRWkzPrTMaA9Xu3B87icznE5LV+mu9imtLSai7v4vggj08 } 19, 53 -- Rs7qrojfLdb4RzjNXUBY1I84NqpPjHj726e6JxsczMfwgrGDIEqh1dHrryTse6rabwJ6 } 19, 53 -- j3fVqfBJBWHNCWthHoc6tnpWKFlrCgbp4Df2sYAVv5BICMHNGLRtZkf3rIL6hkdDDhLO } 19, 53 -- qWo7mPNJdLSM+F3EMjeuOK1Qda8B/wjvxkl0qX8opW6SvjhGlg6tk3duBNp8d2nKWIeI } 19, 53 -- qX3NHmxgOBHTYV2d0TCONioZxxkfCu+buH2sRtuRWyzhobzvkyS3WXf3I655baFi+BSZ } 19, 53 -- H/IQ== } 19, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 19, 53 -- d=1e100.net; s=20161025; } 19, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 19, 53 -- :user-agent:mime-version:in-reply-to:content-language } 19, 53 -- :content-transfer-encoding; } 19, 53 -- bh=mMISr17c45+HxKA6htDQWDJH0Z+vg7iKrjYy2l1RHNE=; } 19, 53 -- b=nooKsUBR+ww7eDKfbpRn+81v9NLrPan//r/5O+buhz9oeRtSewK4R9QUN06+Fo2h0z } 19, 53 -- GKQbUjLBx7RHKstW76W2pW9cxwqH9brPugs9me6S2uxVGPDO94SMaaLBP3I7bAJmG9Na } 19, 53 -- fgDSlctJ4BZtlnnKsIPIlGChm0wHKboasxZdWx08l4L91sdFTG74lcrBwMa1NLLZeWUi } 19, 53 -- RnAuNxRUHCS4Go/K8yVHvrXXJ59G3dJepGCWyxQ86JEbVomX1wthS/rMYn/Gl/GuYZzs } 19, 53 -- jHWFzC7MptLfk2ec3G3eTQgrEbfdE4AAkEOPTap3lIUc3R4+jGZk+QfGprx3DEt7RA5S } 19, 53 -- O+lg== } 19, 53 -- X-Gm-Message-State: AHPjjUhQQIrpn3ssPRlvME1swv2uD9adp6VQCi/vmyJwCA+fUEXA+8qm } 19, 53 -- Zh1+LfJefmUvwJYa } 19, 53 -- X-Google-Smtp-Source: ADKCNb6doJr3P1rqfKHy1JzZ8d94bHblWreIwEgDHerp8+SnFvfF5/ySGMeZEiEQe9HiSAxhDIgwyQ== } 19, 53 -- X-Received: by 10.80.177.141 with SMTP id m13mr4053669edd.114.1505108757708; } 19, 53 -- Sun, 10 Sep 2017 22:45:57 -0700 (PDT) } 19, 53 -- Received: from Nestors-MacBookAir-Pro-2014-ElCapitan-OSX-1011.local ([77.60.103.234]) } 19, 53 -- by smtp.googlemail.com with ESMTPSA id x40sm4550877edb.48.2017.09.10.22.45.56 } 19, 53 -- for {microscopy-at-microscopy.com} } 19, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 19, 53 -- Sun, 10 Sep 2017 22:45:56 -0700 (PDT) } 19, 53 -- Subject: Fwd: Re: [Microscopy] viaWWW:air table for ultramicrotome } 19, 53 -- References: {4ECD6627-7D04-4A49-9D84-A7AAB560C88F-at-cmich.edu} } 19, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 19, 53 -- From: Microscopy Listserver {microscopy.listserver-at-gmail.com} } 19, 53 -- X-Forwarded-Message-Id: {4ECD6627-7D04-4A49-9D84-A7AAB560C88F-at-cmich.edu} } 19, 53 -- Message-ID: {82227c06-d829-9902-e7f0-32d33b4a9d0e-at-gmail.com} } 19, 53 -- Date: Mon, 11 Sep 2017 07:45:55 +0200 } 19, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) } 19, 53 -- Gecko/20100101 Thunderbird/52.3.0 } 19, 53 -- MIME-Version: 1.0 } 19, 53 -- In-Reply-To: {4ECD6627-7D04-4A49-9D84-A7AAB560C88F-at-cmich.edu} } 19, 53 -- Content-Type: text/plain; charset=utf-8; format=flowed } 19, 53 -- Content-Language: en-US } 19, 53 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both researchers4u-at-gmail.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: researchers4u-at-gmail.com Name: S. Shobeikeh
Organization: SHIRAZ university
Title-Subject: [Filtered] ultrasonic bath vs probe sonicator Message: Dear colleagues: We recently decided to purchase a sonicator for our TEM laboratory so we can use it to disperse nanoparticles (like: CNT, Au, Ag, ZnO and so on) in aqueous mediums. As you all know some laboratories use ultrasonic bath and others use probe sonicator. I was wondering based on your experience which of the above mentioned sonicators give the best result for dispersion of nanoparticles. Any input would be appreciated. Best regards
Login Host: 151.232.123.23 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
From traialet662f-at-gmail.com Tue Sep 12 16:57:38 2017 Return-Path: {traialet662f-at-gmail.com} Received: from gmail.com (lync-mediation.hosted.chinatelecomglobal.com [203.14.188.88]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v8CLvZIY032510 for {microscopylistserverarchive6-at-microscopy.com} ; Tue, 12 Sep 2017 16:57:37 -0500 Received: from rsmail.alkoholic.net ([Wed, 13 Sep 2017 05:48:51 +0700]) by external.newsubdomain.com with ASMTP; Wed, 13 Sep 2017 05:48:51 +0700 Message-ID: {084BD048.79319C47-at-gmail.com}
*********************************************************************************** Forwarded from "Ask a Microscopist" Please remember that the person asking the question is likely not a member the listserver, and **any reply should go directly to the poster** as well as to the list. Using the "reply" function in your email does *not* send your answer to the person asking the question. Please copy their email address from their question. ***********************************************************************************
Name:Avinash Both School:University of Nebraska-lincoln Grade/Education Level:Graduate Location:Lincoln, NE US Email:avinash.both-at-huskers.unl.edu
Subject:Temperature controlled Stage for Microscope
Your Question:I want to do study adsorption of gases by nanomaterials using microscopy. For carrying out this experiment I need a temperature controlled sample stage where I can monitor the temperature as well ensure that there is no leakage. Could you please help me with suggestions in what kind of temperature controlled stage should I use for doing this study ? Thanks in advance.
*********************************************************************************** Forwarded from "Ask a Microscopist" Please remember that the person asking the question is likely not a member the listserver, and **any reply should go directly to the poster** as well as to the list. Using the "reply" function in your email does *not* send your answer to the person asking the question. Please copy their email address from their question. ***********************************************************************************
Name:Avinash Both School:University of Nebraska-lincoln Grade/Education Level:Graduate Location:Lincoln, NE US Email:avinash.both-at-huskers.unl.edu
Subject:Temperature controlled Stage for Microscope
Your Question:I want to do study adsorption of gases by nanomaterials using microscopy. For carrying out this experiment I need a temperature controlled sample stage where I can monitor the temperature as well ensure that there is no leakage. Could you please help me with suggestions in what kind of temperature controlled stage should I use for doing this study ? Thanks in advance.
From nyshin-at-daelimcorp.co.kr Fri Sep 15 08:44:42 2017 Return-Path: {nyshin-at-daelimcorp.co.kr} Received: from c06 (host124-231-211-80.serverdedicati.aruba.it [80.211.231.124] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v8FDicS2029690; Fri, 15 Sep 2017 08:44:39 -0500 Received: from 80.211.231.124 (localhost [IPv6:::1]) by c06 (Postfix) with ESMTPA id 7BDA31B3A; Fri, 15 Sep 2017 05:12:13 -0400 (EDT) MIME-Version: 1.0 Content-Type: multipart/mixed; boundary="=_8e646402a1092b73e76018d90295e04d"
I have heard that Dow Corning #3140 RTV Silicone glue can be thinned to paintable consistency with acetic acid(vinegar). It can then be applied as a very thin coating to provide a water-tight seal in a small space. What is the recipe to thin the glue?
regards, Don ============================================= Don Chernoff, Ph.D., President Advanced Surface Microscopy, Inc.; 3250 N. Post Rd., Suite 120; Indianapolis IN 46226 USA E-Mail: donc-at-asmicro.com www.asmicro.com Voice: +1-317-895-5630 Toll free: +1-800-374-8557 (in USA & Canada) Fax: +1-317-895-5652 [business activities since 1990: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.] =============================================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both rim-at-drumagog.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: rim-at-drumagog.com Name: Rim Buntinas
Title-Subject: [Filtered] High Resolution Display on Cambridge S-360
Message: Hi,
Does anyone have experience with adding a high resolution display to an S-360? Our SEM didn't come with one standard, but I have a spare high resolution mixer board and the PLL box from another scope. I tried installing them, and added another store card (4 total now), but I can't seem to get an image. Any help would be appreciated!
I wonder if this option needs changes to the options file on the disk to make it work?
Login Host: 73.73.102.208 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both chrismillertx89-at-gmail.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: chrismillertx89-at-gmail.com Name: Chris Miller
Message: Does anyone know the cost of this hyperspectral microscopy setup and more importantly what microscopes it can be used with? Our lab is interested in whether it can be installed on microscopes we already have.
You will need to define what you mean by hyperspectral microscopy.
That is a spectroscopic data acquisition technique and depends upon the excitation source and the detected signal (light, x-rays, electrons...)
I use electrons in TEM/STEM and it is possible to measure light, x-rays, and electrons, but others wil use as the source SEM, X-rays , Ions, and least we not forget visible photons.
Nestor
=========================================== Dr. Nestor J. Zaluzec Argonne National Laboratory Photon Sciences Division 9700 S. Cass Ave Bldg 212 / A-143 Argonne, Illinois 60439 USA Email: Zaluzec-at-aaem.amc.anl.gov
Tel: 530-NES-TORZ (530-637-8679) has Voice Mail Lab: 630-252-7901 Fax: 630-252-4798
Senior Scientist - Argonne National Laboratory Fellow of the Microscopy Society of America Senior Fellow the Computational Institute - University of Chicago E.P. Wigner Fellow - Oak Ridge National Laboratory Past President Microscopy Society of America Adjunct Professor of Physics - Northern Illinois University & the University of Illinois at Chicago Visiting Professor of Microscopy - Manchester University
From ronaldfaust205xw-at-gmail.com Sun Sep 17 19:19:26 2017 Return-Path: {ronaldfaust205xw-at-gmail.com} Received: from gmail.com (dialin.hosted.chinatelecomglobal.com [203.14.188.89]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v8I0JNkC030136 for {microscopylistserverarchive6-at-microscopy.com} ; Sun, 17 Sep 2017 19:19:25 -0500 Received: from qrx.quickslick.com [125.57.219.148] by qnx.mdrost.com with ASMTP; Mon, 18 Sep 2017 05:45:21 +0500 Received: from rly04.hottestmile.com ([Mon, 18 Sep 2017 05:35:38 +0500]) by smtp18.yenddx.com with NNFMP; Mon, 18 Sep 2017 05:35:38 +0500 Received: from [25.232.170.227] by mxs.perenter.com with QMQP; Mon, 18 Sep 2017 05:31:09 +0500 Message-ID: {0CA9CED4.1ED96A41-at-gmail.com}
Nicklas, Okay got it
MD
-----Original Message----- X-from: Niklas Hoffmann [mailto:nhoffm14-at-jhmi.edu] Sent: Saturday, September 16, 2017 3:19 PM To: Michael Delannoy
X-from: Silverj-at-miamioh.edu
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Silverj-at-miamioh.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Message: I am reaching out to the community to initiate a collaboration with a facility capable of preparing alloy materials for TEM imaging. My facility does not have a dual beam, FIB or electro-chemical polisher. Any of these would be sufficient. I am in Cincinnati, Ohio. Contact me at silverj-at-miamioh.edu. Cheers, Josh Login Host: 184.54.80.104 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both adjerid-at-vt.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: adjerid-at-vt.edu Name: Khaled Adjerid
Organization: Virginia Tech
Title-Subject: [Filtered] Modulus Calculation
Message: Hello Everyone, I was wondering if anyone had an efficient way of taking large amounts of AFM indentation data and quickly calculating modulus (either a matlab script or with Bruker software). I am running a Veeco Bioscope II AFM and taking indents on soft materials (~0.5 gPa). I have 100's of files with Z height and deflection error (Volts) I would like to process and curve fit using the Sneddon-Hertz cone model is tedious to do on each individual file. I have attempted to contact Bruker but they have not gotten back to me either. Is their software capable of doing such a calculation? Best, Khaled Adjerid Virginia Tech Login Host: 73.99.89.78 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
From watkinsmelanie58qyzu-at-gmail.com Tue Sep 19 22:06:41 2017 Return-Path: {watkinsmelanie58qyzu-at-gmail.com} Received: from gmail.com ([182.16.112.202]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v8K36djx030540 for {microscopylistserverarchive6-at-microscopy.com} ; Tue, 19 Sep 2017 22:06:40 -0500 Received: from [130.126.202.222] by mmx09.tilkbans.com with LOCAL; Wed, 20 Sep 2017 03:53:42 +0100 Received: from unknown (181.218.167.242) by smtp.endend.nl with ESMTP; Wed, 20 Sep 2017 03:37:20 +0100 Received: from unknown (HELO mts.locks.grgtween.net) (Wed, 20 Sep 2017 03:23:33 +0100) by mail.webhostings4u.com with ASMTP; Wed, 20 Sep 2017 03:23:33 +0100 Received: from unknown (HELO mts.locks.grgtween.net) (Wed, 20 Sep 2017 03:12:04 +0100) by mail.gimmicc.net with NNFMP; Wed, 20 Sep 2017 03:12:04 +0100 Received: from smtp18.yenddx.com ([Wed, 20 Sep 2017 03:04:07 +0100]) by rly04.hottestmile.com with LOCAL; Wed, 20 Sep 2017 03:04:07 +0100 Message-ID: {660EDF16.83B72949-at-gmail.com}
X-from: jhyun-at-gatan.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jhyun-at-gatan.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: jhyun-at-gatan.com Name: John Hyun
Organization: Gatan
Title-Subject: [Filtered] Advances in In-Situ Microscopy Workshop
Message: Addressing In-Situ TEM Challenges Using Integrated Hardware and Software
Drexel University, Bossone Research Center, Philadelphia , PA 8:00 am - 5:00 pm, Wednesday, October 4, 2017
This intensive workshop at Drexel University, Bossone Research Center, will present and demonstrate state-of-the-art instruments and software for in-situ EM applications. Lectures and hands-on lab sessions will show how a complete in-situ system can optimize data management and reveal critical details in reaction processes. Specifically, temperature induced transistions in both complex oxide materials and 2-dimentional MXene will be studied. Chemical and electronic transistions will be measured with direct detection EELS and correlated with the sample IV behavior.
The workshop is complimentary to all confirmed registrants. Space is limited. Please register by October 2, 2017. Register online: http://info.gatan.com/acton/form/11413/01b5:d-0001/0/-/-/-/-/index.htm?sid=TV2:5xipvspXZ
Login Host: 50.58.128.11 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
There is a TEM (Siemens Elmiskop 102) being decommissioned in Chicago. Its operating status is not known, however, the unit represents historical value as I understand both due to the research that it made possible in addition to its age.
Whether it could be brought online again is another question entirely -- such an endeavor would not be for the faint of heart. Perhaps the best outcome would be historical preservation in a museum or otherwise.
In any event, there is little time before the unit ends up disposed of. Even interest in components for someone operating a similar instrument would be a better fate than it is currently slated for. Additionally, there is little time left before the equipment is dismantled (approximately one week).
If you have any interested in the unit, please feel free to reach out via phone or email.
Regards, Peter Zieba 312-285-3794
==============================Original Headers============================== 6, 42 -- From peter-at-analyticslounge.org Wed Sep 20 11:38:53 2017 6, 42 -- Received: from mail.wickerparkdata.com (mail.wickerparkdata.com [66.90.94.245]) 6, 42 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v8KGcrRl017538 6, 42 -- for {microscopy-at-microscopy.com} ; Wed, 20 Sep 2017 11:38:53 -0500 6, 42 -- Received: from localhost (localhost [127.0.0.1]) 6, 42 -- by mail.wickerparkdata.com (Postfix) with ESMTP id 3FC288156A33 6, 42 -- for {microscopy-at-microscopy.com} ; Wed, 20 Sep 2017 12:33:10 -0500 (CDT) 6, 42 -- Received: from mail.wickerparkdata.com ([127.0.0.1]) 6, 42 -- by localhost (mail.wickerparkdata.com [127.0.0.1]) (amavisd-new, port 10032) 6, 42 -- with ESMTP id 1Z6r-Sof8HqS for {microscopy-at-microscopy.com} ; 6, 42 -- Wed, 20 Sep 2017 12:33:09 -0500 (CDT) 6, 42 -- Received: from localhost (localhost [127.0.0.1]) 6, 42 -- by mail.wickerparkdata.com (Postfix) with ESMTP id 31B858156A36 6, 42 -- for {microscopy-at-microscopy.com} ; Wed, 20 Sep 2017 12:33:09 -0500 (CDT) 6, 42 -- DKIM-Filter: OpenDKIM Filter v2.10.3 mail.wickerparkdata.com 31B858156A36 6, 42 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=analyticslounge.org; 6, 42 -- s=9726369C-8DB4-11E5-9B49-A1F2971FB84A; t=1505928789; 6, 42 -- bh=R4+YuTLkyGuRdLZBOfy9aq/02mLNo4sgD4uo8yd3yas=; 6, 42 -- h=Date:From:To:Message-ID:MIME-Version; 6, 42 -- b=vS3JhQi5L+SsX7sgoKtdQ5MfT+jVnJH7VLGl4bStZUMwXfFc+138hsF+H3c7cr7yr 6, 42 -- nQzcwxiFHKnp8Qk9pCW6ycjOTtJo8brsL2BX3TOPuCfL/p2W8hsFNnVYyKEg73h7nA 6, 42 -- cnEw1bwvCgJWpyyCIMUkUcxb7ZvoFLpxgQfpQTyQ= 6, 42 -- X-Virus-Scanned: amavisd-new at mail.wickerparkdata.com 6, 42 -- Received: from mail.wickerparkdata.com ([127.0.0.1]) 6, 42 -- by localhost (mail.wickerparkdata.com [127.0.0.1]) (amavisd-new, port 10026) 6, 42 -- with ESMTP id Asm3eKQ8GfLJ for {microscopy-at-microscopy.com} ; 6, 42 -- Wed, 20 Sep 2017 12:33:09 -0500 (CDT) 6, 42 -- Received: from mail.wickerparkdata.com (mail.wickerparkdata.com [66.90.94.245]) 6, 42 -- by mail.wickerparkdata.com (Postfix) with ESMTP id DC3338156A33 6, 42 -- for {microscopy-at-microscopy.com} ; Wed, 20 Sep 2017 12:33:08 -0500 (CDT) 6, 42 -- Date: Wed, 20 Sep 2017 12:33:07 -0500 (CDT) 6, 42 -- From: Peter Zieba {peter-at-analyticslounge.org} 6, 42 -- To: microscopy-at-microscopy.com 6, 42 -- Message-ID: {1239401350.41061.1505928787111.JavaMail.zimbra-at-analyticslounge.org} 6, 42 -- Subject: Decomissioned TEM / Siemens Elmiskop 102 6, 42 -- MIME-Version: 1.0 6, 42 -- Content-Type: text/plain; charset=utf-8 6, 42 -- Content-Transfer-Encoding: 7bit 6, 42 -- X-Originating-IP: [66.90.94.245] 6, 42 -- X-Mailer: Zimbra 8.7.1_GA_1670 (ZimbraWebClient - GC60 (Win)/8.7.1_GA_1670) 6, 42 -- Thread-Index: TVEV+AHrLf7GHOi0uYNCeAj8JIplYQ== 6, 42 -- Thread-Topic: Decomissioned TEM / Siemens Elmiskop 102 ==============================End of - Headers==============================
at some time I owned an Elmiskop 101 which is nearly the same like the 101. I still have lots of plans, special gaskets, O-rings, tubes, HV cables etc.
I surely will not be for those faint of heart to get it up to vacuum condition and align it but if anybody needs help doing so, ask me. There are some O-rings where you might never think of, and they might be broken, if the TEM is on air since some years.
It will take ca. two days to get the TEM on pallets (ca. 4 of them ;-) ) and the cross weight will be ca. 4500 pounds...
Best wishes,
Stefan
Am 20.09.2017 um 19:02 schrieb peter-at-analyticslounge.org: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi all, } } There is a TEM (Siemens Elmiskop 102) being decommissioned in Chicago. Its operating status is not known, however, the unit represents historical value as I understand both due to the research that it made possible in addition to its age. } } Whether it could be brought online again is another question entirely -- such an endeavor would not be for the faint of heart. Perhaps the best outcome would be historical preservation in a museum or otherwise. } } In any event, there is little time before the unit ends up disposed of. Even interest in components for someone operating a similar instrument would be a better fate than it is currently slated for. Additionally, there is little time left before the equipment is dismantled (approximately one week). } } If you have any interested in the unit, please feel free to reach out via phone or email. } } Regards, } Peter Zieba } 312-285-3794 } } ==============================Original Headers============================== } 6, 42 -- From peter-at-analyticslounge.org Wed Sep 20 11:38:53 2017 } 6, 42 -- Received: from mail.wickerparkdata.com (mail.wickerparkdata.com [66.90.94.245]) } 6, 42 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v8KGcrRl017538 } 6, 42 -- for {microscopy-at-microscopy.com} ; Wed, 20 Sep 2017 11:38:53 -0500 } 6, 42 -- Received: from localhost (localhost [127.0.0.1]) } 6, 42 -- by mail.wickerparkdata.com (Postfix) with ESMTP id 3FC288156A33 } 6, 42 -- for {microscopy-at-microscopy.com} ; Wed, 20 Sep 2017 12:33:10 -0500 (CDT) } 6, 42 -- Received: from mail.wickerparkdata.com ([127.0.0.1]) } 6, 42 -- by localhost (mail.wickerparkdata.com [127.0.0.1]) (amavisd-new, port 10032) } 6, 42 -- with ESMTP id 1Z6r-Sof8HqS for {microscopy-at-microscopy.com} ; } 6, 42 -- Wed, 20 Sep 2017 12:33:09 -0500 (CDT) } 6, 42 -- Received: from localhost (localhost [127.0.0.1]) } 6, 42 -- by mail.wickerparkdata.com (Postfix) with ESMTP id 31B858156A36 } 6, 42 -- for {microscopy-at-microscopy.com} ; Wed, 20 Sep 2017 12:33:09 -0500 (CDT) } 6, 42 -- DKIM-Filter: OpenDKIM Filter v2.10.3 mail.wickerparkdata.com 31B858156A36 } 6, 42 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=analyticslounge.org; } 6, 42 -- s=9726369C-8DB4-11E5-9B49-A1F2971FB84A; t=1505928789; } 6, 42 -- bh=R4+YuTLkyGuRdLZBOfy9aq/02mLNo4sgD4uo8yd3yas=; } 6, 42 -- h=Date:From:To:Message-ID:MIME-Version; } 6, 42 -- b=vS3JhQi5L+SsX7sgoKtdQ5MfT+jVnJH7VLGl4bStZUMwXfFc+138hsF+H3c7cr7yr } 6, 42 -- nQzcwxiFHKnp8Qk9pCW6ycjOTtJo8brsL2BX3TOPuCfL/p2W8hsFNnVYyKEg73h7nA } 6, 42 -- cnEw1bwvCgJWpyyCIMUkUcxb7ZvoFLpxgQfpQTyQ= } 6, 42 -- X-Virus-Scanned: amavisd-new at mail.wickerparkdata.com } 6, 42 -- Received: from mail.wickerparkdata.com ([127.0.0.1]) } 6, 42 -- by localhost (mail.wickerparkdata.com [127.0.0.1]) (amavisd-new, port 10026) } 6, 42 -- with ESMTP id Asm3eKQ8GfLJ for {microscopy-at-microscopy.com} ; } 6, 42 -- Wed, 20 Sep 2017 12:33:09 -0500 (CDT) } 6, 42 -- Received: from mail.wickerparkdata.com (mail.wickerparkdata.com [66.90.94.245]) } 6, 42 -- by mail.wickerparkdata.com (Postfix) with ESMTP id DC3338156A33 } 6, 42 -- for {microscopy-at-microscopy.com} ; Wed, 20 Sep 2017 12:33:08 -0500 (CDT) } 6, 42 -- Date: Wed, 20 Sep 2017 12:33:07 -0500 (CDT) } 6, 42 -- From: Peter Zieba {peter-at-analyticslounge.org} } 6, 42 -- To: microscopy-at-microscopy.com } 6, 42 -- Message-ID: {1239401350.41061.1505928787111.JavaMail.zimbra-at-analyticslounge.org} } 6, 42 -- Subject: Decomissioned TEM / Siemens Elmiskop 102 } 6, 42 -- MIME-Version: 1.0 } 6, 42 -- Content-Type: text/plain; charset=utf-8 } 6, 42 -- Content-Transfer-Encoding: 7bit } 6, 42 -- X-Originating-IP: [66.90.94.245] } 6, 42 -- X-Mailer: Zimbra 8.7.1_GA_1670 (ZimbraWebClient - GC60 (Win)/8.7.1_GA_1670) } 6, 42 -- Thread-Index: TVEV+AHrLf7GHOi0uYNCeAj8JIplYQ== } 6, 42 -- Thread-Topic: Decomissioned TEM / Siemens Elmiskop 102 } ==============================End of - Headers==============================
-- ----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Is there anybody in this community having a Ziess Libera TEM? How do you get service for repairs and maintenance form Ziess given that Ziess stops manufacturing TEMs?
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mjaklewi-at-yorku.ca as well as the Microscopy Listserver ---------------------------------------------------------------------------
does anybody collect images of unsuccessfully coated grids? I have polymeric substrate carbon activated grids (purchased) - I have difficulty to understand what is on them... looks like I do not even need a specimen, there is plenty to analyze as it is .... I will be returning them - but I would love to take a look at some typical (and atypical) failures during the grid preparation,
have a lovely day
Magdalena
Login Host: 130.63.104.250 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
From donnedua0sc-at-gmail.com Fri Sep 22 22:35:53 2017 Return-Path: {donnedua0sc-at-gmail.com} Received: from gmail.com ([222.97.39.119]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v8N3Zojr023678 for {microscopylistserverarchive6-at-microscopy.com} ; Fri, 22 Sep 2017 22:35:52 -0500 Received: from relay-x.misswldrs.com [47.33.159.112] by mxs.perenter.com with ESMTP; Fri, 22 Sep 2017 18:38:24 -0900 Received: from rly04.hottestmile.com ([182.32.229.69]) by rsmail.alkoholic.net with SMTP; Fri, 22 Sep 2017 18:28:17 -0900 Received: from relay37.vosimerkam.net ([Fri, 22 Sep 2017 18:20:00 -0900]) by smtp.mixedthings.net with NNFMP; Fri, 22 Sep 2017 18:20:00 -0900 Received: from [82.105.219.31] by asx121.turbo-inline.com with QMQP; Fri, 22 Sep 2017 18:14:59 -0900 Message-ID: {E7B18F43.6E4B5F6D-at-gmail.com}
X-from: chrisbrantner-at-gwu.edu
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both chrisbrantner-at-gwu.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: chrisbrantner-at-gwu.edu Name: Chris Brantner
Message: The George Washington University Nanofabrication and Imaging Center will be hosting a Correlative Microscopy workshop November 7-10 in Washington DC. If you are interested in more details, please visit our site.
https://nic.gwu.edu/microscopy-workshop
Chris Brantner
Login Host: 173.167.206.130 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
Is there anybody in this community having a Ziess Libera TEM? How do you get service for repairs and maintenance form Ziess given that Ziess stops manufacturing TEMs?
I also had one. It was an excellent TEM. I also still have some parts, including the desk/panel units and the complete roll-out pumping system.
You should contact Alan Hawk (alan.j.hawk.civ-at-mail.mil) at the National Museum of the History of Medicine, which is located in Silver Spring, MD.
NMHM has one of nearly every Siemens TEM in their collection (among many other TEMs), and Alan might be interested.
BTW: I have three Siemens Elmiskop TEMs, one of which is a Ia from 1964, and is back in operating condition. I ask the list if anyone knows of another Siemens TEM still in operation.
Mike Marko MSA Archivist
On 9/20/2017 1:29 PM, diller-at-stefan-diller.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Peter, } } at some time I owned an Elmiskop 101 which is nearly the same like the } 101. I still have lots of plans, special gaskets, O-rings, tubes, HV } cables etc. } } I surely will not be for those faint of heart to get it up to vacuum } condition and align it but if anybody needs help doing so, ask me. There } are some O-rings where you might never think of, and they might be } broken, if the TEM is on air since some years. } } It will take ca. two days to get the TEM on pallets (ca. 4 of them ;-)Â } ) and the cross weight will be ca. 4500 pounds... } } } Best wishes, } } Stefan } } } } Am 20.09.2017 um 19:02 schrieb peter-at-analyticslounge.org: } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Hi all, } } } } There is a TEM (Siemens Elmiskop 102) being decommissioned in Chicago. Its operating status is not known, however, the unit represents historical value as I understand both due to the research that it made possible in addition to its age. } } } } Whether it could be brought online again is another question entirely -- such an endeavor would not be for the faint of heart. Perhaps the best outcome would be historical preservation in a museum or otherwise. } } } } In any event, there is little time before the unit ends up disposed of. Even interest in components for someone operating a similar instrument would be a better fate than it is currently slated for. Additionally, there is little time left before the equipment is dismantled (approximately one week). } } } } If you have any interested in the unit, please feel free to reach out via phone or email. } } } } Regards, } } Peter Zieba } } 312-285-3794 } } } } ==============================Original Headers============================== } } 6, 42 -- From peter-at-analyticslounge.org Wed Sep 20 11:38:53 2017 } } 6, 42 -- Received: from mail.wickerparkdata.com (mail.wickerparkdata.com [66.90.94.245]) } } 6, 42 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v8KGcrRl017538 } } 6, 42 -- for {microscopy-at-microscopy.com} ; Wed, 20 Sep 2017 11:38:53 -0500 } } 6, 42 -- Received: from localhost (localhost [127.0.0.1]) } } 6, 42 -- by mail.wickerparkdata.com (Postfix) with ESMTP id 3FC288156A33 } } 6, 42 -- for {microscopy-at-microscopy.com} ; Wed, 20 Sep 2017 12:33:10 -0500 (CDT) } } 6, 42 -- Received: from mail.wickerparkdata.com ([127.0.0.1]) } } 6, 42 -- by localhost (mail.wickerparkdata.com [127.0.0.1]) (amavisd-new, port 10032) } } 6, 42 -- with ESMTP id 1Z6r-Sof8HqS for {microscopy-at-microscopy.com} ; } } 6, 42 -- Wed, 20 Sep 2017 12:33:09 -0500 (CDT) } } 6, 42 -- Received: from localhost (localhost [127.0.0.1]) } } 6, 42 -- by mail.wickerparkdata.com (Postfix) with ESMTP id 31B858156A36 } } 6, 42 -- for {microscopy-at-microscopy.com} ; Wed, 20 Sep 2017 12:33:09 -0500 (CDT) } } 6, 42 -- DKIM-Filter: OpenDKIM Filter v2.10.3 mail.wickerparkdata.com 31B858156A36 } } 6, 42 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=analyticslounge.org; } } 6, 42 -- s=9726369C-8DB4-11E5-9B49-A1F2971FB84A; t=1505928789; } } 6, 42 -- bh=R4+YuTLkyGuRdLZBOfy9aq/02mLNo4sgD4uo8yd3yas=; } } 6, 42 -- h=Date:From:To:Message-ID:MIME-Version; } } 6, 42 -- b=vS3JhQi5L+SsX7sgoKtdQ5MfT+jVnJH7VLGl4bStZUMwXfFc+138hsF+H3c7cr7yr } } 6, 42 -- nQzcwxiFHKnp8Qk9pCW6ycjOTtJo8brsL2BX3TOPuCfL/p2W8hsFNnVYyKEg73h7nA } } 6, 42 -- cnEw1bwvCgJWpyyCIMUkUcxb7ZvoFLpxgQfpQTyQ= } } 6, 42 -- X-Virus-Scanned: amavisd-new at mail.wickerparkdata.com } } 6, 42 -- Received: from mail.wickerparkdata.com ([127.0.0.1]) } } 6, 42 -- by localhost (mail.wickerparkdata.com [127.0.0.1]) (amavisd-new, port 10026) } } 6, 42 -- with ESMTP id Asm3eKQ8GfLJ for {microscopy-at-microscopy.com} ; } } 6, 42 -- Wed, 20 Sep 2017 12:33:09 -0500 (CDT) } } 6, 42 -- Received: from mail.wickerparkdata.com (mail.wickerparkdata.com [66.90.94.245]) } } 6, 42 -- by mail.wickerparkdata.com (Postfix) with ESMTP id DC3338156A33 } } 6, 42 -- for {microscopy-at-microscopy.com} ; Wed, 20 Sep 2017 12:33:08 -0500 (CDT) } } 6, 42 -- Date: Wed, 20 Sep 2017 12:33:07 -0500 (CDT) } } 6, 42 -- From: Peter Zieba {peter-at-analyticslounge.org} } } 6, 42 -- To: microscopy-at-microscopy.com } } 6, 42 -- Message-ID: {1239401350.41061.1505928787111.JavaMail.zimbra-at-analyticslounge.org} } } 6, 42 -- Subject: Decomissioned TEM / Siemens Elmiskop 102 } } 6, 42 -- MIME-Version: 1.0 } } 6, 42 -- Content-Type: text/plain; charset=utf-8 } } 6, 42 -- Content-Transfer-Encoding: 7bit } } 6, 42 -- X-Originating-IP: [66.90.94.245] } } 6, 42 -- X-Mailer: Zimbra 8.7.1_GA_1670 (ZimbraWebClient - GC60 (Win)/8.7.1_GA_1670) } } 6, 42 -- Thread-Index: TVEV+AHrLf7GHOi0uYNCeAj8JIplYQ== } } 6, 42 -- Thread-Topic: Decomissioned TEM / Siemens Elmiskop 102 } } ==============================End of - Headers============================== }
As a general rule, once the OEM drops support of your instrument for one reason or another you go to one of third-party support organizations. Try reaching out to service engineers who were servicing your instrument while it was supported - even if they are not allowed to help you, they could know who can. Network with used equipment dealers and speak to other TEM users in your region to find out support options in your geographic location. Google for similar instrument around the world and contact tool owners to find out where they obtain support. You could also try posting similar request in relevant LinkedIn user groups.
Keep in mind that decent third-party service is *not* cheaper then OEM support. Depending on your geographical location and also age, condition, and degree of neglect of your instrument, cost of T&M service call for bringing it back to service could be out of reach...
Good luck!
Valery Ray =========================== PBS&T, MEO Engineering Company 290 Broadway, Suite 298 Methuen, MA 01844, USA Phone: +1-978-305-0479 - leave a message Mobie: +1-978-305-0479 - leave a message E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
} From: "microscopy.listserver-at-gmail.com" } |--microscopy.listserver-at-gmail.= } com--| } To: vray-at-partbeamsystech.com=20 } Sent: Saturday, September 23, 2017 } 10:07 AM } Subject: [Microscopy] Fwd: Asking for } help for Ziess Libera TEM } =20 } } } } ---------------------------------------------------------------------------= } - } The Microscopy ListServer -- } CoSponsor:=C2=A0 The Microscopy Society of Ame= } rica } To=C2=A0 Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyLists= } erver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------= } - } } X-from: =C2=A0=C2=A0=C2=A0 Shahrzad } Hosseini |--shahrzad.hosseini-at-gmail.com--| } } } } } Hi All, } } Is there anybody in this community } having a Ziess Libera TEM? How do you ge= } t service for repairs and=20 } maintenance form Ziess given that Ziess } stops manufacturing TEMs? } } Thanks, } Shahrzad
==============================Original Headers============================== 7, 26 -- From vray-at-partbeamsystech.com Sat Sep 23 14:07:37 2017 7, 26 -- Received: from sonic316-47.consmr.mail.bf2.yahoo.com (sonic316-47.consmr.mail.bf2.yahoo.com [74.6.130.221]) 7, 26 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v8NJ7aTc005336 7, 26 -- for {microscopy-at-microscopy.com} ; Sat, 23 Sep 2017 14:07:36 -0500 7, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1506197511; bh=vk83Jxve030Uix8kiA7pcqusfATGjSqAHvu2myqY1ak=; h=Date:From:Reply-To:To:Subject:References:From:Subject; b=q4f8+5KacLulV8Jid+FvC0goLxJSW/ogJWZuKd7i95J8X1YmZVOSYr07UY3zjZ98vtJx4mJOJ2Q5cCgGfdhu3WgbHYWME5ZDPSBFJKlulHPZ2gYnfe3PzEojPTnFSimQvID9yQfE2LctE02MB3Am2K5tAqaL3Z7au7InmLgXjIQrhOC/QPk8NtaKtz+tCR+t5vkG+BfkqD1boAV7LRn526byKpxHy9Umu06CJ6WBR8udKs7T6QTD7uIEG2a2udOrSDUUtIloHmneelpjgIpVxXsIAisBtflgYjKVal77saLt9CVF9MnlA5oTvW4jdB1+JNjaO79DqzPRQs5WOpqqIQ== 7, 26 -- X-YMail-OSG: lrV1zq0VM1mWAVMTv4WyTHQuhQiuXUh_5mJCcHq2aFqAzJMboacbLc5NMECpvfK 7, 26 -- Yr67blh6mblSTggVpmfuShpxpq4ma0g2eT8vI_rl_EW.lm47WrPYLG0Kj_wK7UOZFc.5N05kFKmK 7, 26 -- IvrnUTFwPkrEDlyLevKCRfqKljiN7y9k_Tr56VkqQH8FmDqNIMhy3Px1zs2kcVWjflkaUm8pjCQ5 7, 26 -- IECQEeGCTnUTm6leSXmhrJVGxpvPwxkSe7hcDOXsPSr5sbtbXMo4VFZ1NKRv3H85TguBFIVwvxfP 7, 26 -- XJ9h9oUTuqZpxVqzBMB4qgUk6cfz8bpFwLqzRMLNo1rBAAQL5wKhXSQ6T0QWdLpEXKX71YVhVkD0 7, 26 -- 0VOO92zIJ8IkLjxRiXS_n1YIK0kyTdsBdADd69HOwfzcmB1rA_jSh3G7vHIMDz0FNCDyUEMGlYK9 7, 26 -- Ff7.QqHZ6zlBLNMt9MJlRMIgbIcfXxCl.IF96NjwrfCSrn6nZTLkNCcd0x5xd_hPITJ3Gf77N8m0 7, 26 -- SgOzeDtjnwz7FJg-- 7, 26 -- Received: from sonic.gate.mail.ne1.yahoo.com by sonic316.consmr.mail.bf2.yahoo.com with HTTP; Sat, 23 Sep 2017 20:11:51 +0000 7, 26 -- Date: Sat, 23 Sep 2017 20:07:50 +0000 (UTC) 7, 26 -- From: {vray-at-partbeamsystech.com} 7, 26 -- Reply-To: {vray-at-partbeamsystech.com} 7, 26 -- To: {microscopy-at-microscopy.com} 7, 26 -- Message-ID: {648843848.1996691.1506197270687-at-mail.yahoo.com} 7, 26 -- Subject: Re: Fwd: Asking for help for Ziess Libera TEM 7, 26 -- MIME-Version: 1.0 7, 26 -- Content-Type: text/plain; charset=UTF-8 7, 26 -- References: {648843848.1996691.1506197270687.ref-at-mail.yahoo.com} 7, 26 -- X-Mailer: WebService/1.1.10495 YahooMailBasic Mozilla/5.0 (Windows NT 10.0; WOW64; rv:55.0) Gecko/20100101 Firefox/55.0 7, 26 -- Content-Transfer-Encoding: 8bit 7, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v8NJ7aTc005336 ==============================End of - Headers==============================
From janiceking866vig-at-gmail.com Sun Sep 24 06:42:58 2017 Return-Path: {janiceking866vig-at-gmail.com} Received: from gmail.com ([211.218.42.141]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v8OBgtUp011837 for {microscopylistserverarchive6-at-microscopy.com} ; Sun, 24 Sep 2017 06:42:57 -0500 Received: from unknown (HELO mx.reskind.net) (Mon, 25 Sep 2017 00:27:12 +1200) by public.micromail.com.au with SMTP; Mon, 25 Sep 2017 00:27:12 +1200 Received: from mmx09.tilkbans.com [36.9.83.234] by smtp.mixedthings.net with ESMTP; Mon, 25 Sep 2017 00:13:16 +1200 Received: from smtp.mixedthings.net ([24.54.252.101]) by asx121.turbo-inline.com with LOCAL; Mon, 25 Sep 2017 00:11:35 +1200 Received: from [141.122.220.148] by external.newsubdomain.com with ESMTP; Sun, 24 Sep 2017 23:59:14 +1200 Message-ID: {F2C35608.EFDDB5A3-at-gmail.com}
X-from: krspba-at-elkem.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both krspba-at-elkem.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: krspba-at-elkem.com Name: Pl Baggethun
Organization: Elkem
Title-Subject: [Filtered] Embedding powder for SEM - alternatives to epoxy
Message: Purpose: Embed powders for studies in plane section by SEM. Powder particles range in size typically 1 um to 100 um.
Problem: Standard epoxy for metallographic specimen preparation has high basicity and reacts with some acidic mineral powders such as Si. Question: Are there alternatives to epoxy that are also viscous enough to make random sections, that is, the particles must not be allowed to migrate in the resin after mixing?
Login Host: 144.127.1.1 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
Does anyone have information on how to align the lenses on a Cambridge S-360? We just starting using this SEM and would love to get the column aligned. It seems that Optibeam isn’t doing what it should and I wonder if the condenser lenses need alignment. I appreciate the help!
Dear Colleagues, Please share the enclosed position announcement with anyone you know who may be interested. Thank you, Stefanie
Assistant professor position in Paleoclimatology/Geochemistry
The Department of Earth and Environmental Studies at Montclair State University (New Jersey) invites applications for a full-time (10-month) tenure-track Assistant Professor position in Paleoclimatology/Geochemistry. We seek individuals who work with geological archives (corals, speleothems, ocean sediments, lake sediments, ice cores, glacial deposits, tree rings) to understand Earth’s climate processes such as atmospheric and ocean circulation, the global carbon cycle, cryosphere dynamics and how these processes affect biological and/or human systems, over a range of time scales. The successful candidate will develop a vigorous externally funded research program, engage in student mentoring, and have a strong commitment to excellence in teaching. The candidate will play a critical role in our bachelors, masters, and doctoral programs in Earth and Environmental Science, Geography, Sustainability Science, and Environmental Management. Teaching responsibilities will include introductory courses in Earth and Environmental Science, as well as upper-level undergraduate and graduate courses within the applicant’s areas of expertise. Service to the department, university, and larger professional community is required.
Qualifications & Requirements Applicants are expected to have a Ph.D. in Earth and Environmental Science, Earth System Science, Geology, Geochemistry, Geography, or other appropriate field, a record of peer-reviewed scholarship in paleoclimatology and geochemistry, and evidence of potential for success in grant activity. Post-doctoral experience is desirable.
Anticipated Start Date: September 1, 2018 Apply: Electronic applications assembled into one combined .pdf or .doc file, and containing a cover letter, CV, statements of teaching and research interests, and the names and contact information for three professional references should be sent to EAESsearch-at-montclair.edu. Please include position number V-F24.
Apply By: October 20, 2017
********************* Dr. Stefanie Brachfeld Acting Associate Dean for Academic Affairs, College of Science and Mathematics Montclair State University Montclair, NJ 07043 phone: (973) 655-5129 brachfelds-at-mail.montclair.edu *********************
I am looking for a solution to replace our Olympus Morada camera on a Tecnai Biotwin (80kV). We have been happy with this camera for past 10 years, but the CCD is deteriorating and there is no hard/software support available any more.
It would be very helpful if you can let me know your thoughts on similar kind of cameras currently available on the market with reasonable price. Users are cell biologists, so a large CCD chip and fast searching mode is necessary, the high resolution is not required. I am also thinking to add a similar one on our Tecnai F20 for the similar purpose.
Thanks very much in advance.
Xinran N. Liu ________________________________________
Xinran Nick Liu, M.D. & Ph.D. Director of Center for Cellular & Molecular Imaging Bio & Cryo Electron Microscopy Core Facility Yale University School of Medicine Office: 203.785.4050 Lab: 203.785.5390 http://medicine.yale.edu/ccmi/em
I've been running my Cambridge S200 (pretty much the same column) for almost 30 years. If the instrument has been sitting for a while, you should pull out, disassemble and thoroughly clean the column liners, gun assembly and apertures. The alignment is a repetitive "tweaking" of aperture position and electronic alignment controls throughout a wide range of current settings.
We had an old S360 just for parts. We may have some circuit boards that we were going to dispose of. If we still have them, they are yours for the taking.
Al Stone WJE
*Alan Stone** *Principal
*Wiss, Janney, Elstner Associates, Inc. *** /Engineers /|/Architects / |/Materials Scientists/ 200 Larkin Drive, Suite A, Wheeling, Illinois 60090
tel 847.353.8100 | fax 847.353.8204 direct 847.753.6352 | mobile 847.636.1300 www.wje.com {http://www.wje.com/} __
_astone-at-wje.com {mailto:astone-at-wje.com} _
*/Formerly with ASTON Metallurgical Services Company/* www.astonmet.com {http://www.astonmet.com}
This confidential message is for the private use of the recipient as shown. If this has been delivered to you in error, then please return the message and delete from your files. Thank you.
On Tue, Sep 26, 2017 at 6:03 AM, {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote:
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver On-Line {http://www.microscopy.com/MicroscopyListserver On-Line} Help http://www.microscopy.com/MicroscopyListserver/FAQ.html {http://www.microscopy.com/MicroscopyListserver/FAQ.html} ----------------------------------------------------------------------------
X-from: Rim Buntinas {rim-at-drumagog.com {mailto:rim-at-drumagog.com} }
Hi,
Does anyone have information on how to align the lenses on a Cambridge S-360? We just starting using this SEM and would love to get the column aligned. It seems that Optibeam isn’t doing what it should and I wonder if the condenser lenses need alignment. I appreciate the help!
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both tom.strader-at-microscopyinnovations.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: tom.strader-at-microscopyinnovations.com Name: Tom Strader
Presented by: Midwest Microscopy and Microanalysis Society (M3S) A local affiliate of the Microscopy Society of America and the Microanalysis Society
Hosted by the Medical College of Wisconsin
Friday, October 13th, 2017 Medical College of Wisconsin 8701 W Watertown Plank Rd, Milwaukee, WI 53226 Meeting location: Building A Parking is free Located in Visitor's lot
Onsite Registration Fee: Meeting Free for M3S members, $20.00 for non-members, $5.00 for students (Fee includes M3S membership for 2017/2018) Please RSVP by Tuesday, October 10th to: Secretary-at-midwestmicroscopy.org
*********************************************
8:00 8:45a Registration 9:00 9:10 a Welcome and Opening Remarks 9:10 9:55a Recent advances in confocal imaging (SIM, TIRF) including tissue clearing & co-localization Robert Price - Research Professor of Cell Biology & Anatomy, Director of the Instrumentation Resource Facility (IRF), University of South Carolina School of Medicine. (http://dba.med.sc.edu/price.asp) 9:55 10:40a Cryo-EM tomography of RNA virus genome replication organelles Paul Ahlquist - Paul J. Kaesberg Professor of Molecular Virology and Oncology at the University of Wisconsin, Madison, and John and Jeanne Rowe Chair in Virology at the Morgridge Institute for Research. (http://www.hhmi.org/scientists/paul-ahlquist) presented by 10:40 11:10a Break Visit with Vendors 11:10 11:55a - Multiscale imaging of the cellular microenvironment Kevin Eliceiri - Director, Laboratory for Optical and Computational Instrumentation (LOCI Center) UW Madison (https://loci.wisc.edu/people/kevin-eliceiri) 11:55 - 1:10p Lunch Visit with Vendors 1:10 1:30p Microtubules and the ciliary photoreceptor Tylor Lewis - Graduate Student, Medical College of Wisconsin, Department of Ophthalmology & Visual Sciences 1:30 1:50p Imaging and quantifying mitochondrial morphology Megan C. Harwig - Research Scientist, Medical College of Wisconsin, Department of Biochemistry 1:50 2:10p In vivo and ex vivo imaging of the ground squirrel retina Benjamin S. Sajdak - Graduate Student, Medical College of Wisconsin, Department of Neuroscience, Advanced Ocular Imaging Program 2:10 2:30p Total Internal Reflection Fluorescence Microscopy - TIRF Chen Chen - Postdoctoral Fellow, Medical College of Wisconsin, Barbieri Lab
Login Host: 76.250.40.37 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
From michcons458rypah-at-gmail.com Mon Oct 2 14:40:05 2017 Return-Path: {michcons458rypah-at-gmail.com} Received: from gmail.com ([222.243.203.234]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v92Je25W017337 for {microscopylistserverarchive6-at-microscopy.com} ; Mon, 2 Oct 2017 14:40:04 -0500 Received: from rly04.hottestmile.com ([187.220.20.122]) by mx03.listsystemsf.net with LOCAL; Mon, 02 Oct 2017 14:38:25 -0600 Received: from smtp.mixedthings.net [72.233.199.107] by mail.gimmicc.net with LOCAL; Mon, 02 Oct 2017 14:20:21 -0600 Received: from [63.48.152.220] by mail.webhostings4u.com with QMQP; Mon, 02 Oct 2017 14:08:25 -0600 Received: from [200.103.211.156] by smtp-server1.cfdenselr.com with ESMTP; Mon, 02 Oct 2017 14:00:30 -0600 Message-ID: {489D601E.BD2B873A-at-gmail.com}
X-from: rz2371-at-columbia.edu
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both rz2371-at-columbia.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: rz2371-at-columbia.edu Name: Rui Zu
Organization: Columbia University
Title-Subject: [Filtered] How to deblur STEM images
Message: Hi, all. I got the STEM images from Talos F200X, the parameters of images are 200kv, condenser aperture 50, 10Mx, condenser lens 205mm, spot size 9, acquisition time 20s. I have tried low-pass filter, and currently, I am coding wiener deconvolution in MATLAB, but it requires Point Spread Function. How do you estimate the point spread function or how to make your images clearer? Login Host: 128.59.149.215 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
The Annual Meeting of Michigan Microscopy & Microanalysis Society and Michigan Center for Materials Characterization Open House Wednesday, November 1st 2017
Abstracts may be submitted via: http://www.michmicroscopy.org/paper.php Deadline for submission: September 29th, 2017 (now extended to October 10th 2017)
Location: University of Michigan North Campus ResearchComplex, Dining Hall Building 18, 2800 Plymouth Rd, Ann Arbor MI 48109-2800
Schedule: 8:30 - 9:00 Registration and breakfast 9:00 - 9:05 Welcome and introduction 9:05 - 10:35 Keynote speakers 10:50 - 12:20 Contributed presentations 12:20 - 14:30 Luncheon Guided tours of (MC)2, poster session, micrograph contest, visiting vendors 14:30 - 16:45 Further presentations 16:45 - 17:00 Poster and micrograph competition awards 17:00 - 17:30 MMMS Business meeting
Keynote presentations include:
Determining atomic structure across scale & dimensions with highly convergent electron beams Prof. Robert Hovden, Materials Science and Engineering, University of Michigan
Using cryo-electron microscopy to visualize the function of PMP22, a major cause of Charcot-Marie-Tooth disease (CMTD) Prof. Melanie Ohi, Life Sciences Institute, University of Michigan Shaping (and reshaping) biological membrane architecture for vertebrate photoreceptor health (and disease) Prof. Andrew Goldberg, Biomedical Sciences, Oakland University
Ultrasonic Fatigue and its role in Materials Centric Design Dr. Jason W. Carroll, Eaton Corporation
For details and questions contact John Mansfield, jfmjfm-at-umich.edu ____ John Mansfield PhD Cphys MInstP Director of Education & Engagement Michigan Center for Materials Characterization Building 28, Room 3045W, University of Michigan 2800 Plymouth Road, Ann Arbor MI 48109-2800 USA Phone: (734) 936-3352 Cell. Phone: (734) 834-3913 Email: jfmjfm-at-umich.edu
==============================Original Headers============================== 13, 54 -- From microscopy.listserver-at-gmail.com Wed Oct 4 06:14:22 2017 13, 54 -- Received: from mail-io0-f195.google.com (mail-io0-f195.google.com [209.85.223.195]) 13, 54 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v94BELMT003036 13, 54 -- for {microscopy-at-microscopy.com} ; Wed, 4 Oct 2017 06:14:21 -0500 13, 54 -- Received: by mail-io0-f195.google.com with SMTP id f202so1216997ioe.3 13, 54 -- for {microscopy-at-microscopy.com} ; Wed, 04 Oct 2017 05:19:16 -0700 (PDT) 13, 54 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 13, 54 -- d=gmail.com; s=20161025; 13, 54 -- h=subject:references:to:from:message-id:date:user-agent:mime-version 13, 54 -- :in-reply-to:content-language:content-transfer-encoding; 13, 54 -- bh=Jz3UNOgHmtr5tjSeUSVzPL9dYXcxkCBXO+dQwBdfADY=; 13, 54 -- b=aylbZ5uaHKz/CVHC/fxIn0FkQNoGmAuQEEahQmdtfUZNtIB1ihomHCByYKMlgLgBIi 13, 54 -- HTpx6Aebwl4wY54AdXmJXpcCTqQcrWC4Sh99BuiKYfrQyh3V004sMmeoR8sKR8jr/Szz 13, 54 -- TTH2oPI9jfTozTKgIFiATleOpwyb9Bctewu6lDT+k51gKwa6kzN6/5DEz9Rn6SdG2XBf 13, 54 -- JqHYGimAQdxlDh9zCCeu1gLwL250mFUYy7bIw9hSi/DqTZ9C4fWxqDdPO76XmP5dmKGf 13, 54 -- JelboBevBkcrt8pAh7jZPVA14FbBIjMRHH9uXJbFbNGnGGLBeqxb95kUkjenZXxvT3Ju 13, 54 -- cxFA== 13, 54 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 13, 54 -- d=1e100.net; s=20161025; 13, 54 -- h=x-gm-message-state:subject:references:to:from:message-id:date 13, 54 -- :user-agent:mime-version:in-reply-to:content-language 13, 54 -- :content-transfer-encoding; 13, 54 -- bh=Jz3UNOgHmtr5tjSeUSVzPL9dYXcxkCBXO+dQwBdfADY=; 13, 54 -- b=bBOmCnuD3cQcohbARX1sK5U/oLIJqbx67X+wfLvRn1+oBWuGQD+tFlRoHWak52K9gG 13, 54 -- NrJhs7F3BEPuz+yDIl2xKrUf4flfqFqbI1h5gg7Pf+mtyZ4acGZt6hQEbsHm2TqmdKUX 13, 54 -- eRfA1VSIE21ETH/zUQ4KS75NRinXg3U4I4v/fq8Qu//CvPIu6ASBFJuFPHarEMVk+yjC 13, 54 -- o0nouQ5xHP6nxbS61NUSS2dDt9xTrPWxWm3Ap4lVJVTpFCddgH3sulZEMUWQe+7T7p16 13, 54 -- FfixDZR8jUTgVsboWd0r+6iZ30GT562CdO+/6hwaaMryMd8f+9NrOjzckDmHPp0IdR51 13, 54 -- ldWA== 13, 54 -- X-Gm-Message-State: AMCzsaVLYuGzg47hNOB8vRZhntkdvFPD56ca9E5uKDAm71NFKMUBxk1u 13, 54 -- oGGiVn5A0eWS49jLnSxqciuH4AOf 13, 54 -- X-Google-Smtp-Source: AOwi7QC/SVVto3O4ObuPsLDlvzIUmG5P7x8PgHQnhxmHH9cPqe/xB3BhmJ+oJzvK8EmW653kpq6NMw== 13, 54 -- X-Received: by 10.107.11.139 with SMTP id 11mr1141831iol.271.1507119556118; 13, 54 -- Wed, 04 Oct 2017 05:19:16 -0700 (PDT) 13, 54 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:8c40:e263:bcc6:837b]) 13, 54 -- by smtp.googlemail.com with ESMTPSA id 79sm3295557itl.34.2017.10.04.05.19.15 13, 54 -- for {microscopy-at-microscopy.com} 13, 54 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 13, 54 -- Wed, 04 Oct 2017 05:19:15 -0700 (PDT) 13, 54 -- Subject: Fwd: Annual Meeting Michigan Microscopy & Microanalysis Society and 13, 54 -- Michigan Center for Materials Characterization Open House 13, 54 -- References: {6EC78216-8A71-4A3A-B649-6685E55830ED-at-me.com} 13, 54 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 13, 54 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} 13, 54 -- X-Forwarded-Message-Id: {6EC78216-8A71-4A3A-B649-6685E55830ED-at-me.com} 13, 54 -- Message-ID: {5c84d74d-7606-76ac-2f53-e6e82c604d07-at-gmail.com} 13, 54 -- Date: Wed, 4 Oct 2017 07:19:14 -0500 13, 54 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) 13, 54 -- Gecko/20100101 Thunderbird/52.3.0 13, 54 -- MIME-Version: 1.0 13, 54 -- In-Reply-To: {6EC78216-8A71-4A3A-B649-6685E55830ED-at-me.com} 13, 54 -- Content-Type: text/plain; charset=windows-1252; format=flowed 13, 54 -- Content-Language: en-US 13, 54 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I would like to image water-based pigment dyes for inkjet printing.
There might be some tricks to spread the dyes as thin as possible on a specially prepared glass substrate. Should I use Polysine-coated slides or will the Van der Waals forces to their thing on the nano-sized particles on every smooth surface?
Thanks,
Stefan
--
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both bonnie_salyer-at-tedpella.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Material Science Professional
Message: We are looking for qualified candidates to be considered for Materials Science Product Specialist with Ted Pella, Inc. located in beautiful Redding, California. If you have a degree in Material Science and have hands-on SEM and TEM experience, and have been considering a career move, please read on.
This is an exciting opportunity for a Material Science expert to be involved in the product development and growth of our MS, Forensic, AFM and SEM product lines. We have been serving the microscopy community for nearly 50 years, we have a great team, and we are located in an amazing rural city, free from the hassles of big city life. If any of this sounds good to you, or someone you know, please take a moment to review this opportunity at www.tedpella.com Bonnie Salyer bonnie_salyer-at-tedpella.com Ted Pella, Inc.
Login Host: 12.7.209.242 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
From johnsonloretta061loyiu-at-gmail.com Mon Oct 9 08:14:26 2017 Return-Path: {johnsonloretta061loyiu-at-gmail.com} Received: from gmail.com ([27.50.138.67]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v99DEMsk011568 for {microscopylistserverarchive6-at-microscopy.com} ; Mon, 9 Oct 2017 08:14:24 -0500 Received: from [80.228.132.123] by relay37.vosimerkam.net with ESMTP; Mon, 09 Oct 2017 18:59:55 +0600 Received: from public.micromail.com.au ([Mon, 09 Oct 2017 18:48:53 +0600]) by public.micromail.com.au with LOCAL; Mon, 09 Oct 2017 18:48:53 +0600 Received: from [37.82.171.243] by relay37.vosimerkam.net with ASMTP; Mon, 09 Oct 2017 18:33:45 +0600 Message-ID: {05A2B7FA.D43BF3E2-at-gmail.com}
Hi all
I'm preparing some documentation for a SEM course and I want to give a sort of glossary I begin some time ago, of "all" (or more modestly, a typical sampling of...) SEM detector acronyms used on the different manufacturers / machines, like Upper/Lower, Top, SE1/SE2, ETD, BSD, Compo, etc.
It's sometime a puzzle for beginner (and trained people too) to find what type of electrons does the YWK detector from the SEM-999 collect, or what are the differences between the YWK-a and the YWK-u detector !
So may I ask if some SEM users could send me the following informations : -manufacturer, model, and age of your SEM -acronym of the different detectors avaible on it, with their full meaning -the kind of signal they collect and the type of informations they give.
If I get such return for only 15-20 machines, and with what I've soon collected, it can give a usefull list !
Thanks in advance, and I'll send a feadback when it is done.
Best regards Jacques
--
J. Faerber IPCMS-DSI Institut de Physique et Chimie des Matériaux de Strasbourg Département Surfaces et Interfaces 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
*Company Description:*This highly respected privately held, multi-disciplined environmental laboratory testing firm with a national presence has been around for several decades, working with high end, loyal clientele across the gamut of Fortune 500, industrial, commercial, institutions, government, engineering and consulting firms.
*Job Description: *The core competencies would be the full gamut of Laboratory Management duties in relation to managing, mentoring, developing programs, etc... that will drive the lab to its' highest success.
This exciting opportunity combines the best of working with the resources of an established national firm and the nimbleness of a privately held firm where you could still "put your stamp on it". **
**
This Laboratory Manager will:
*
Manage all aspects of the environmental laboratory operations, analysis, preparation, QA/QC, etc.
*
Preferred experience related to microbial, industrial hygiene, indoor air quality, asbestos and Polarized Light Microscopy ( PLM )
*
Must be experienced with quality control, quality assurance (QA/QC) and daily operations of a laboratory responsible for testing environmental samples
*
Supervise and train new staff
*
Ensure QA/QC policies and proceduresand compliance
*
Ensure data is high quality, clients are serviced, and lab operations are smooth and efficient
*
Conduct reviews and audits to ensure overall program efficiency which includes examination of all testing data, policies and procedures
*
*Requirements: *BS in Life Sciences - Microbiology or Biology or related. Minimum 3 years of supervisory experience with laboratory knowledge and 5 years of analytical experience
#
#
*
**
Sincerely,
Paul Palazzolo Senior Managing Partner
/One of the nation's leading Environmental Search Firms/
//
(Toll Free) 866.712.1810
Paul-at-JPAmri.com {mailto:PaPaul-at-JPAmri.com}
www.JPAmri.com {http://www.JPAmri.com}
Invite me to LinkedIn at:
http://www.linkedin.com/in/paulpalazzolo
* *P* Please consider the environment before printing my e-mail***//**//**//*
From trojlita384boyq-at-gmail.com Tue Oct 10 11:38:47 2017 Return-Path: {trojlita384boyq-at-gmail.com} Received: from gmail.com ([120.210.204.27]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v9AGcf4k005082 for {microscopylistserverarchive6-at-microscopy.com} ; Tue, 10 Oct 2017 11:38:46 -0500 Received: from [21.168.12.192] by relay-x.misswldrs.com with ASMTP; Tue, 10 Oct 2017 22:18:53 +0700 Received: from qrx.quickslick.com ([Tue, 10 Oct 2017 22:09:23 +0700]) by m1.gns.snv.thisdomainl.com with QMQP; Tue, 10 Oct 2017 22:09:23 +0700 Received: from m1.gns.snv.thisdomainl.com ([Tue, 10 Oct 2017 21:57:21 +0700]) by rsmail.alkoholic.net with ASMTP; Tue, 10 Oct 2017 21:57:21 +0700 Received: from unknown (HELO rsmail.alkoholic.net) (Tue, 10 Oct 2017 21:54:37 +0700) by mx.reskind.net with LOCAL; Tue, 10 Oct 2017 21:54:37 +0700 Received: from unknown (66.74.142.50) by qnx.mdrost.com with NNFMP; Tue, 10 Oct 2017 21:36:41 +0700 Message-ID: {50C23DEE.DB25EDF9-at-gmail.com}
X-from: Wendy Salmon {wsalmon-at-wi.mit.edu}
Dear Fellow Microscopists,
New England Society of Microscopy is excited to announce our Special 50^th Anniversary of NESM Fall Symposium and Workshops/Business Meeting, which will be held at the University of Massachusetts, Amherst on November 8th and 9th, 2017. Please find the linked flyer.
The symposium will be a two day program including workshops by Nikon on November 8th and technical talks by New England researchers on the 9th.
Information about meetings details and registration can be found at: http://nesmicroscopy.org/upcoming-meetings/ {https://nesmicroscopy.wildapricot.org/EmailTracker/LinkTracker.ashx?linkAndRecipientCode=jqqgwubZ1tB9%2bDl%2f3DEjKn7qMym9OYqCa9t6M21dagevHFmZs24uMCg2em%2fudFr9O9fJb%2bGzZ6yMYmGaRpQ68LBydqCFO%2b4RjSrsZqxxiVM%3d}
This year we have a great selection of technical talks and poster session.
If you have any questions regarding the poster session, please contact info-at-nesmicroscopy.org {mailto:info-at-nesmicroscopy.org} .
We hope to see you in November at UMass, Amherst!
Cheers,
NESM Board
NESM fall symposium.pdf {https://nesmicroscopy.wildapricot.org/EmailTracker/LinkTracker.ashx?linkAndRecipientCode=a0qQJ8xGDNKzPonorGjrw0we5nMSSzTWwxTu%2f8JHlVPRuj5%2bnFRFosetssaNe3keMh5%2bzHAqZIpH8ScfpDEhpzJl13geoBaGrn85FMhthg0%3d}
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both bassimn-at-mcmaster.ca as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: bassimn-at-mcmaster.ca Name: Nabil Bassim
Organization: McMaster University
Title-Subject: [Filtered] Postdoc position in Advanced Microscopy of Steel Microstructure and Thin Films
Message: A postdoctoral position is available in the performance of two distinct projects. The researcher will use the state-of-the-art aberration-corrected TEM's at the Canadian Centre for Electron Microscopy (ccem.mcmaster.ca) located at McMaster University, Hamilton Canada. The CCEM is a world-class imaging facility and is equipped with 4 TEM's, including two aberation-corrected microscopes (FEI Titans). The first project is to study precipitation mechanisms of Nb-rich steels after hot rolling. This work requires knowledge of chemical imaging, diffraction methods, and an understanding of strain measurement. The second project is the study of the thin film growth process, from 2-D materials up to ALD-grown films. This requires high-resolution imaging (TEM and STEM), EELS, and EDS. The postdoc will be part of the Bassim research group, with a strong collaboration with Prof. Zurob's research group.
Applicants should have a strong background in electron microscopy, preferably with aberration-corrected (S)TEM experience. Additional background in metallurgy or thin films is a bonus. Ability to work in a team, mentor graduate students, as well as excellent verbal and written communication skills are expected.
McMaster University is located in Hamilton, Ontario, within a 45 minute drive from downtown Toronto, which a world class city and 45 minute drive to Niagara Falls.
To apply, please send a cover letter, curriculum vitae including references, and 1-2 relevant published papers to the e-mail below.
Nabil Bassim Associate Professor Department of Materials Science and Engineering, JHE 258 McMaster University 1280 Main Street West Hamilton, ON L8S 4L8 CANADA bassimn-at-mcmaster.ca
Login Host: 130.113.74.83 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
==============================Original Headers============================== 13, 54 -- From microscopy.listserver-at-gmail.com Wed Oct 11 08:32:31 2017 13, 54 -- Received: from mail-io0-f170.google.com (mail-io0-f170.google.com [209.85.223.170]) 13, 54 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v9BDWVs5030942 13, 54 -- for {microscopy-at-microscopy.com} ; Wed, 11 Oct 2017 08:32:31 -0500 13, 54 -- Received: by mail-io0-f170.google.com with SMTP id 97so1904566iok.7 13, 54 -- for {microscopy-at-microscopy.com} ; Wed, 11 Oct 2017 06:27:03 -0700 (PDT) 13, 54 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 13, 54 -- d=gmail.com; s=20161025; 13, 54 -- h=subject:references:to:from:message-id:date:user-agent:mime-version 13, 54 -- :in-reply-to:content-language:content-transfer-encoding; 13, 54 -- bh=EuwkPMBfdh6aI4nGrGXjHH2aWReu1D90MCN1iu5FpMU=; 13, 54 -- b=LE+/ub+vjFtHK/NzK1/+PS0iMrpI0Yy0ji/4M8yywOQUAqPfaTChrRMkc7Lz3aJdzG 13, 54 -- W7S+X09Ke9VNFO4AOPIg4+iioOSJ1XPP9a4rAEeJoGbm8Q90tJ42uj2VpmSbBzweKORd 13, 54 -- sZMHd927uDP+/MvtFF83zyWHzzZVbnQ/lnx4bp5uHoecwXzxSZ+oVI7i5UsDfKdk6gi/ 13, 54 -- OTRdTEz29QC+Pocm6BkapI7H/5ZpXN9oIHDSogM2+TSqtmtABCetbpE8s6oEUoZGm0QI 13, 54 -- G1UiX7WnjC6nvzmn/1kJGHJakuwpOwvfpLDTuwXXaQBt4DAy22As8aLsOv7gMKcqYIBU 13, 54 -- AzDw== 13, 54 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 13, 54 -- d=1e100.net; s=20161025; 13, 54 -- h=x-gm-message-state:subject:references:to:from:message-id:date 13, 54 -- :user-agent:mime-version:in-reply-to:content-language 13, 54 -- :content-transfer-encoding; 13, 54 -- bh=EuwkPMBfdh6aI4nGrGXjHH2aWReu1D90MCN1iu5FpMU=; 13, 54 -- b=S6Dj0kEps9HREEDGpOb7faW9CNYsVIi9fbKWseBFouvnu5TACc6bOliGEYBQkZ/btH 13, 54 -- NXKAMZwJ3OFWbclnjcAEHla2U3V/bGh43baeB5GMBhDfNzTnBIRvUCKrde2eBAftq2Vf 13, 54 -- VA/cmeUZIzPDYVDelJXvU4hoUv4Re/5AImu4K0hlHX7PjOmW9XA8HaqUOPvEDYGrLZS0 13, 54 -- y9CX+WuDhfIkytVyGFGmn31qAhDs/OD+JG+ilHnvRCaWXFklHTHajUOtl5od74GlPB+K 13, 54 -- XOPTxZ33Dc8rf8cX6YRper8KKLyr8oLQnhpv+J2/VpavNcUb86Rhikpm0hbP2+TE+s2+ 13, 54 -- N3lw== 13, 54 -- X-Gm-Message-State: AMCzsaW+Vq41o+gIRWAWfoBk+O1gAiRnqistltoIH61/ioRQLbDMwn1u 13, 54 -- 0pSD+4XNAwX4DgCXrOEXlEo1QVx1 13, 54 -- X-Google-Smtp-Source: AOwi7QBvn+Ts403duKgwSF/370fk0RYc1n3MHwg4Q3qpszOidc6y97tY4KFiTISTuojUew6mIW8k6g== 13, 54 -- X-Received: by 10.107.201.204 with SMTP id z195mr13485224iof.16.1507728422632; 13, 54 -- Wed, 11 Oct 2017 06:27:02 -0700 (PDT) 13, 54 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:93d:26df:b76c:3369]) 13, 54 -- by smtp.googlemail.com with ESMTPSA id j5sm6488023ita.34.2017.10.11.06.27.02 13, 54 -- for {microscopy-at-microscopy.com} 13, 54 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 13, 54 -- Wed, 11 Oct 2017 06:27:02 -0700 (PDT) 13, 54 -- Subject: viaWWW: Postdoc position in Advanced Microscopy of Steel 13, 54 -- Microstructure and Thin Films 13, 54 -- References: {201710101452.v9AEq4UU002902-at-microscopy.com} 13, 54 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 13, 54 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} 13, 54 -- X-Forwarded-Message-Id: {201710101452.v9AEq4UU002902-at-microscopy.com} 13, 54 -- Message-ID: {3c148eeb-60b9-7d76-dc71-7c00b5764192-at-gmail.com} 13, 54 -- Date: Wed, 11 Oct 2017 08:27:01 -0500 13, 54 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) 13, 54 -- Gecko/20100101 Thunderbird/52.3.0 13, 54 -- MIME-Version: 1.0 13, 54 -- In-Reply-To: {201710101452.v9AEq4UU002902-at-microscopy.com} 13, 54 -- Content-Type: text/plain; charset=windows-1252; format=flowed 13, 54 -- Content-Language: en-US 13, 54 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Does anyone know a lab that want to be paid to look for wollastonite in rubber stock? We have a client that want a quote for this service. The problem, as it's been explained to me, is they want to know more than if there are particles of Ca silicate present. They want to know is it wollastonite. We don't have PLM or X-ray diffraction, so ARDL can't do it.
Any comments or suggestions would be helpful.
Thanks and advance!
Stay safe...........
Frank Karl Microscopist Akron Rubber Development Laboratory 2887 Gilchrist Road Akron, Ohio 44305
==============================Original Headers============================== 8, 56 -- From frank_karl-at-ardl.com Wed Oct 11 09:52:32 2017 8, 56 -- Received: from NAM02-SN1-obe.outbound.protection.outlook.com (mail-sn1nam02on0059.outbound.protection.outlook.com [104.47.36.59]) 8, 56 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v9BEqWqh010304 8, 56 -- for {microscopy-at-microscopy.com} ; Wed, 11 Oct 2017 09:52:32 -0500 8, 56 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 8, 56 -- d=ardlcorp.onmicrosoft.com; s=selector1-ardl-com; 8, 56 -- h=From:Date:Subject:Message-ID:Content-Type:MIME-Version; 8, 56 -- bh=bJ0YhoW4wb4St8tqOUHbAZQFvsPhFKtl/h/qSYQtxBg=; 8, 56 -- b=K3CyK88lqmgqWhIU8tL+XYwKZPGFqEA5g3OIPym1HMVSV+ygqdkuuVmM49ZsU0O2PvRGF9jWXDqmVWwh9YIfi11W1D22/FK0F2wJx9ucgMmHyQZD0vkSbljKkKsjYgjvqY5cnsB21QXdXhd1y2UMDUMHyAu7GrR4cnRbd+wEjZ8= 8, 56 -- Received: from MWHPR15MB1152.namprd15.prod.outlook.com (10.175.2.18) by 8, 56 -- MWHPR15MB1151.namprd15.prod.outlook.com (10.175.2.17) with Microsoft SMTP 8, 56 -- Server (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_256_CBC_SHA384_P256) id 8, 56 -- 15.20.77.7; Wed, 11 Oct 2017 14:47:03 +0000 8, 56 -- Received: from MWHPR15MB1152.namprd15.prod.outlook.com ([10.175.2.18]) by 8, 56 -- MWHPR15MB1152.namprd15.prod.outlook.com ([10.175.2.18]) with mapi id 8, 56 -- 15.20.0077.020; Wed, 11 Oct 2017 14:47:03 +0000 8, 56 -- From: Frank Karl {frank_karl-at-ardl.com} 8, 56 -- To: "'Microscopy Listserver (microscopy-at-microscopy.com)'" 8, 56 -- {microscopy-at-microscopy.com} 8, 56 -- Subject: Wollastonite in rubber 8, 56 -- Thread-Topic: Wollastonite in rubber 8, 56 -- Thread-Index: AdNCn5WrOBCaYdJ0SnWURrW2jI+1mw== 8, 56 -- Date: Wed, 11 Oct 2017 14:47:02 +0000 8, 56 -- Message-ID: {MWHPR15MB115241D0513D3CA01AE658FC954A0-at-MWHPR15MB1152.namprd15.prod.outlook.com} 8, 56 -- Accept-Language: en-US 8, 56 -- Content-Language: en-US 8, 56 -- X-MS-Has-Attach: 8, 56 -- X-MS-TNEF-Correlator: 8, 56 -- authentication-results: spf=none (sender IP is ) 8, 56 -- smtp.mailfrom=frank_karl-at-ardl.com; 8, 56 -- x-originating-ip: [74.207.215.218] 8, 56 -- x-ms-publictraffictype: Email 8, 56 -- x-microsoft-exchange-diagnostics: 1;MWHPR15MB1151;6:yZKmQTLkr+s3zms47QQzNMGpKn1ZbAvuE7Ko+NqW+1tZyTi/E+1khEirs/9uxBCahyz5SF0IukWf4NBqF4oKqKQLLwjKZjUSUh2Gmk0s+dBEt3wIAWEWFZecsbNmwW2eB7tECLl++wGMRBhJk6QYZ6MflgDkMUzZje41qgJwmiAsVU00OgYBa2LBZ/4HKwMfVx1TOjhHAbPiDMKm2TYfYPsIKvjjAlpUUZkDP+zVQyb2YjHp4i2fS1I4LGhO8PzjI/+Rc1j++e3vx2R0rPaIkX0b7bO1BS/jw+IlARtBnJCiEersk76IQaZP36ll5E1FshSkhF9Qw4TU6H5EiCSD1A==;5:VtfuAiYoZ9WokKTc/v3vbPRvcU+X+9Izgs3+dJvnTLfO4jz2n/D6/gWAXWOhLLmHwQGzZ9asc+W2iwB59fqRXf5DP55JaJrI6lLuLFB+Uf99HBqIcoUK+wKfyTzpuzJDvQ19EXSJvl04dJPNADQQuw==;24:uzMh1cAnQYM0vU3jRZLGKNOVk7fKNKoqPGdyjwbxeumOctLxxMggA4RHlUvCdbqHiENJeKCQGnxtfH8b2P9TfD+r0TOL6bUAb4n4moj0vK0=;7:6DCns7eG8ZpHuN3zBPi+t0LlRmzTIogU5B/hF+IYuKclDW2g1JHiX7//MkNVo/+DhrlTjODDrz4Y/W6lpUGw0Wgcb5EsWqZRWj39Ycjxc5uVlvmG77hK/Hmzf1yikv7IgEoARpzczAssppvJFlhPKzWJgd/KvOW1nFa30w/6RGvPJiDHa7Yqs34Ucea4nA/2Elt+AEy2t3+n4YV8//oe1SnT3Nm0rag72avXNc2/LPk= 8, 56 -- x-ms-exchange-antispam-srfa-diagnostics: SSOS; 8, 56 -- x-ms-office365-filtering-correlation-id: 0a714b8f-ca20-4093-68e6-08d510b6ef91 8, 56 -- x-microsoft-antispam: UriScan:;BCL:0;PCL:0;RULEID:(22001)(2017030254152)(2017082002075)(2017052603199)(201703131423075)(201702281549075);SRVR:MWHPR15MB1151; 8, 56 -- x-ms-traffictypediagnostic: MWHPR15MB1151: 8, 56 -- x-exchange-antispam-report-test: UriScan:; 8, 56 -- x-microsoft-antispam-prvs: {MWHPR15MB115141B53A362A931545EDBA954A0-at-MWHPR15MB1151.namprd15.prod.outlook.com} 8, 56 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:(100000700101)(100105000095)(100000701101)(100105300095)(100000702101)(100105100095)(6040450)(2401047)(5005006)(8121501046)(93006095)(93001095)(100000703101)(100105400095)(3002001)(10201501046)(6041248)(20161123560025)(201703131423075)(201702281528075)(201703061421075)(201703061406153)(20161123564025)(2016111802025)(20161123558100)(20161123555025)(20161123562025)(6043046)(6072148)(201708071742011)(100000704101)(100105200095)(100000705101)(100105500095);SRVR:MWHPR15MB1151;BCL:0;PCL:0;RULEID:(100000800101)(100110000095)(100000801101)(100110300095)(100000802101)(100110100095)(100000803101)(100110400095)(100000804101)(100110200095)(100000805101)(100110500095);SRVR:MWHPR15MB1151; 8, 56 -- x-forefront-prvs: 0457F11EAF 8, 56 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10009020)(6009001)(376002)(346002)(39830400002)(199003)(189002)(7116003)(316002)(5660300001)(25786009)(9686003)(97736004)(189998001)(7696004)(14454004)(53936002)(3480700004)(3280700002)(68736007)(77096006)(3660700001)(54356999)(6916009)(105586002)(2906002)(3846002)(7736002)(8936002)(8676002)(86362001)(55016002)(101416001)(74316002)(305945005)(33656002)(106356001)(66066001)(50986999)(81156014)(6506006)(6436002)(81166006)(2900100001)(478600001)(99286003)(102836003)(6116002);DIR:OUT;SFP:1101;SCL:1;SRVR:MWHPR15MB1151;H:MWHPR15MB1152.namprd15.prod.outlook.com;FPR:;SPF:None;PTR:InfoNoRecords;A:1;MX:1;LANG:en; 8, 56 -- received-spf: None (protection.outlook.com: ardl.com does not designate 8, 56 -- permitted sender hosts) 8, 56 -- spamdiagnosticoutput: 1:99 8, 56 -- spamdiagnosticmetadata: NSPM 8, 56 -- Content-Type: text/plain; charset="us-ascii" 8, 56 -- MIME-Version: 1.0 8, 56 -- X-OriginatorOrg: ardl.com 8, 56 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 11 Oct 2017 14:47:03.0763 8, 56 -- (UTC) 8, 56 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted 8, 56 -- X-MS-Exchange-CrossTenant-id: f7eefedd-c7bd-4ea4-91b9-09668a66d0be 8, 56 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: MWHPR15MB1151 8, 56 -- Content-Transfer-Encoding: 8bit 8, 56 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v9BEqWqh010304 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both pscallio-at-dal.ca as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: pscallio-at-dal.ca Name: Pat Scallion
Organization: Dalhousie University
Title-Subject: [Filtered] wearing a pacemaker while using field Emission SEMs or FIBs
Message: Hello all,
I was wondering what experience you have had, of a user with an MRI compatible pacemaker and leads, using and maintaining an electron microscope with ion pumps for the column vacuums? We have a Hitachi S-4700 and a Hitachi FB-2000A from 2003.
Is it a big NO, can it be done with a distance from the system maintained, is there any shielding that can be worn, or any other concerns that I am forgetting? What issues may be encountered?
Regards, Pat Scallion
Login Host: 142.134.154.95 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
==============================Original Headers============================== 12, 53 -- From microscopy.listserver-at-gmail.com Fri Oct 13 20:52:19 2017 12, 53 -- Received: from mail-it0-f43.google.com (mail-it0-f43.google.com [209.85.214.43]) 12, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v9E1qJ9d023454 12, 53 -- for {microscopy-at-microscopy.com} ; Fri, 13 Oct 2017 20:52:19 -0500 12, 53 -- Received: by mail-it0-f43.google.com with SMTP id l196so12498940itl.4 12, 53 -- for {microscopy-at-microscopy.com} ; Fri, 13 Oct 2017 18:46:59 -0700 (PDT) 12, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 12, 53 -- d=gmail.com; s=20161025; 12, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version 12, 53 -- :in-reply-to:content-language:content-transfer-encoding; 12, 53 -- bh=jzfvVu+6a8tUTLRccKHl88nNAhUOM+AbZApBLLQT3Zo=; 12, 53 -- b=XaLbk4mLs/8CLBpky9Aa9pS6TxykZgGK/vwseTY1t8QMxutKXsdeieWi9ebPmH3PHr 12, 53 -- tK2dbZdISq8JcBGJciOTpqcMkgsxtQPjasHVttuKHXosKZhdME0Q89vw80lg6rI4kSYB 12, 53 -- it41FvZdzNtRzotGe+uFaPpK2wru2m2g206KK/ZwExrkJ47f8xGPffVPYiuj1TUhasFg 12, 53 -- iv2MWAoUV/dlAJ8w1QEe4w0Frx8zQDwZRxCIOOpgrbLweChb4XsvQxHnX0MuBBlFiSou 12, 53 -- YAVOtiXlECjr6P016VzVDnpYl91rWKIvAIcFBeeBSgLqMNfYHBqk/49pD8iB31JnOBAJ 12, 53 -- nG/Q== 12, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 12, 53 -- d=1e100.net; s=20161025; 12, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date 12, 53 -- :user-agent:mime-version:in-reply-to:content-language 12, 53 -- :content-transfer-encoding; 12, 53 -- bh=jzfvVu+6a8tUTLRccKHl88nNAhUOM+AbZApBLLQT3Zo=; 12, 53 -- b=QRnZXl/7FwGgrm+nC87tz//7OCq5xGuasj/du4GhJQArWPyjbhk76QDJ8uEVpjsjHp 12, 53 -- YBxZ/5xEItOFh9oTuB3QBJ/EFi0KWojjHmYLvtnrsprezdAaD9Jj93W89hWCh2LBmF+7 12, 53 -- XhA/voY+QCwNIJADhzYGakmOSlppiisrg0hkQgWKXsSZ0eSvJwqc5BJCjEdWW+GZRvlf 12, 53 -- /n1ctq6LFqyxnx/Eu+K1vniu5Vzjxasbwmlq91DNlBfMh1JvsZKjry2ELs4VoObBEhmP 12, 53 -- gPpYDrxTccJcc53vaYAANGr6ahLoyZXhI7OOMNGJFJSj7ZBGalatzMSwmyHT0MhXD2YA 12, 53 -- RhHw== 12, 53 -- X-Gm-Message-State: AMCzsaXXsHLRycCvr52N9Qyz7TaOu++D5BJ67FnKt26vsaEHUTVmODOI 12, 53 -- jUaj0PfLIFkK/D5/vGQDktZLKiEP 12, 53 -- X-Google-Smtp-Source: AOwi7QB8LnDhEPFi9To/kA1x96NSFmryz5cvkk4GNj+AUQKPTCDHA+A2jO9jzK5HwLVKYn9p5IXwpQ== 12, 53 -- X-Received: by 10.36.3.141 with SMTP id e135mr3238697ite.1.1507945618950; 12, 53 -- Fri, 13 Oct 2017 18:46:58 -0700 (PDT) 12, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:91e1:346e:6341:ee46]) 12, 53 -- by smtp.googlemail.com with ESMTPSA id d1sm1145253ioc.29.2017.10.13.18.46.57 12, 53 -- for {microscopy-at-microscopy.com} 12, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 12, 53 -- Fri, 13 Oct 2017 18:46:58 -0700 (PDT) 12, 53 -- Subject: viaWWW:wearing a pacemaker while using field Emission SEMs or FIBs? 12, 53 -- References: {201710131211.v9DCBnPM017174-at-microscopy.com} 12, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 12, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} 12, 53 -- X-Forwarded-Message-Id: {201710131211.v9DCBnPM017174-at-microscopy.com} 12, 53 -- Message-ID: {c9a0832f-8d5d-35a5-feab-4bb957b53df8-at-gmail.com} 12, 53 -- Date: Fri, 13 Oct 2017 20:46:57 -0500 12, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) 12, 53 -- Gecko/20100101 Thunderbird/52.4.0 12, 53 -- MIME-Version: 1.0 12, 53 -- In-Reply-To: {201710131211.v9DCBnPM017174-at-microscopy.com} 12, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed 12, 53 -- Content-Language: en-US 12, 53 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I assume that you are worried about the user and not the imaging. The magnetic fields around (i.e. outside) the instrument would come primarily from the ion pumps and even those are pretty well contained. Also any stray fields are static i.e. not time varying. The field levels the user would encounter in an MRI *FAR* exceed what you will find around an SEM plus they are repetitive so they would have a much stronger effect on the user's pacemaker.
If anything I'd be more worried about the user affecting the imaging rather than the SEM affecting the user.
Cheers, Henk
--------------------
Hendrik O. Colijn Center for Electron Microscopy and AnalysiS The Ohio State University 1305 Kinnear Rd, Suite 100, Columbus, OH 43212
colijn.1-at-osu.edu 614/643-3458 cemas.osu.edu
"Time is that quality of nature which keeps things from happening all at once." (Ray Cummings - 1922) Lately it doesn't seem to be working.
------ Original Message ------ X-from: microscopy.listserver-at-gmail.com To: colijn.1-at-osu.edu Sent: 10/13/2017 9:54:10 PM
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both leandro.lemgrubersoares-at-glasgow.ac.uk as well as the Microscopy Listserver ---------------------------------------------------------------------------
Organization: Wellcome Centre for Molecular Parasitology - University of Glasgow
Title-Subject: [Filtered] LSM5 Live
Message: Hi everyone
We are interested in a Zeiss LSM5 Live module for some in vivo imaging. Does anyone has this module that we could purchase from? Or know where we could get one?
Thanks!
Leandro
Login Host: 130.209.127.194 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
I want to thank everyone who responded to my request for information on analysis and identification of wollastonite. I've forwarded the information and had a lot of fun deleting the out of town messages. Looks like I'll get to do that again.....
Stay safe...........
Frank Karl Microscopist Akron Rubber Development Laboratory 2887 Gilchrist Road Akron, Ohio 44305
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both j-hornick-at-northwestern.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: j-hornick-at-northwestern.edu Name: Jessica E Hornick
Organization: Biological Imaging Facility/Northwestern University
Title-Subject: [Filtered] Imaging Specialist position open at NU
Message: Hello,
The Biological Imaging Facility at Northwestern's Evanston campus is currently hiring for an Imaging Specialist.
The job posting is available here: https://careers.northwestern.edu/psp/hr92prod_er/EMPLOYEE/HRMS/c/HRS_HRAM.HRS_APP_SCHJOB.GBL?Page=HRS_APP_SCHJOB&Action=U&FOCUS=Applicant&SiteId=1
Or contact Jessica Hornick at j-hornick-at-northwestern.edu for more details.
Login Host: 165.124.144.96 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Lett.j.m-at-wustl.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Message: We have two TMC MICRO-g Series 65 vibration isolation tables available immediately at no charge. They have already been dismantled in order to remove the electron microscopes they supported. Photos and other general information can be sent upon request.
Small table (custom-built with custom-placed supports) -- Platform: 55 in x 35 in (3 in thick) -- Four supports: each approximately 15 in x 7 in x 7 in
Large table (TMC MICRO-g model #65-29604-0) -- Platform: 84 in x 56 in at widest points (5 in thick) -- Four supports: each approximately 16 in x 9 in x 9 in
The recipient will be responsible for all moving and shipping costs. (We can put you in contact with a company already familiar with the equipment.) Removal is required by Wednesday, November 15th.
Please let me know of any interest. Thank you.
Jaclynn Lett Senior Research Technician Department of Otolaryngology Washington University School of Medicine 4523 Clayton Avenue, Campus Box 8115 St. Louis, MO 63110 Office: 314-747-7257 Fax: 314-747-7230 Email: lett.j.m-at-wustl.edu Website: http://oto.wustl.edu/Research/Research-Home
Login Host: 128.252.79.225 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both amelia.liu-at-monash.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: amelia.liu-at-monash.edu Name: Amelia Liu
Organization: Monash University
Title-Subject: [Filtered] cryo-FIBSEM position at Monash University
Message: The application closing date for a staff member / Senior Research Officer position to run our newly acquired cryo-Helios G4 UX FIBSEM and FIBSEM-related projects within the Monash Ramaciotti Centre for Cryo-Electron Microscopy has been extended to November 12th.
The Ramaciotti Centre houses a suite of microscopes including a Titan Krios (Gatan K2, energy filter, Volta phase plate, Falcon 3), a Talos Arctica (Volta phase plate, Falcon 3), Tecnai Spririt, Jeol 1400Plus, and Nova NanoSEM. The centre supports and collaborates on EM projects ranging from single particle cryo EM, cryo-tomography, immuno EM to correlative light and electron microscopy.
Monash University is located next to the Australian Synchrotron and hosts excellent infrastructure and expertise in microscopy and imaging through the Monash Centre for Electron Microscopy, the Monash Ramaciotti Centre for Cryo-EM, Monash Micro Imaging, the Melbourne Centre for Nanofabrication, Monash Biomedical Imaging and the ARC Centre of Excellence in Advanced Molecular Imaging.
Senior Research Officer - Cryo-FIBSEM Remuneration: AU$96,230 - AU$106,221 pa HEW Level 08 (plus 17% employer superannuation) Closing date: Sunday 12 November 2017, 11.55pm AEDT Duration: Initial three year fixed-term appointment Enquiries: georg.ramm-at-monash.edu or +61 3 99051280 Link to the job advertisement and application: http://careers.pageuppeople.com/513/cw/en/job/566030/senior-research-officer-cryoem
Login Host: 130.194.141.81 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
From isendana0wveyb-at-gmail.com Fri Oct 20 04:16:13 2017 Return-Path: {isendana0wveyb-at-gmail.com} Received: from gmail.com ([103.231.105.246]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v9K9GB7K023935 for {microscopylistserverarchive6-at-microscopy.com} ; Fri, 20 Oct 2017 04:16:12 -0500 Received: from rsmail.alkoholic.net [167.91.135.251] by public.micromail.com.au with ESMTP; Thu, 19 Oct 2017 21:52:47 -1100 Received: from [186.72.116.149] by mtu67.syds.piswix.net with QMQP; Thu, 19 Oct 2017 21:38:46 -1100 Received: from unknown (137.35.101.2) by mxs.perenter.com with SMTP; Thu, 19 Oct 2017 21:23:39 -1100 Message-ID: {514EF5A5.DF705D26-at-gmail.com}
X-from: jpshield-at-uga.edu
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jpshield-at-uga.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: jpshield-at-uga.edu Name: John Shields
Organization: University of Georgia
Title-Subject: [Filtered] Consulting for HREM architectural workspaces
Message: We are looking for some suggestions of reputable engineering/architectural firms that can advise and consult with university-contracted architectural firms on design of a new high resolution EM space that will potentially hold four HREMs and ancillary equipment.
Please respond directly to jpshield-at-uga.edu
Thanks! John S UGA Athens
Login Host: 198.137.20.67 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
Assistant or Associate Professor-Nanoscience and Nanoengineering
The South Dakota School of Mines & Technology invites applications for a 9-month tenure-track faculty position in Nanoscience and Nanoengineering at the Assistant or Associate Professor level in the area of computational nanoscience, imaging informatics and bioinformatics. Candidates with expertise in other areas relating to bio-nanoscience will also be considered.
The position is associated with a statewide research center: BioSystems Networks / Translational Research (BioSNTR). BioSNTR is focused on advanced fluorescence and optical imaging methods to leverage bioinformatics towards the understanding of signaling networks and their regulation in living systems. BioSNTR is a collaboration between research university partners South Dakota State University, the University of South Dakota, and South Dakota Mines, with multiple industrial partners, including Sanford Research.
The successful candidate will be provided a competitive salary and access to center staff and shared resources, including high performance computing infrastructure. The selected candidate will be expected to contribute to the South Dakota Mines Nanoscience and Nanoengineering PhD program by developing a robust extramurally funded research program, teaching and developing relevant graduate courses, mentoring and advising PhD students, and building strong collaborative research teams.
Specific interests for this position are candidates who could contribute to multiscale modeling, analyzing high volume microscopy data generated by our newly constructed lattice light sheet microscope, and connecting this data with computational biology and next-generation sequencing. Individuals whose research emphasis can complement on-going efforts in biophotonics, cellular level biophysics, super-resolution imaging methods, mechanobiology, and the combination of AFM and fluorescence microscopy for correlative imaging of bio-systems are particularly encouraged to apply.
Applicants must possess a PhD in a science or engineering discipline closely aligned with Nanoscience and Nanoengineering and/or one of the above-mentioned research emphases. Candidates from underrepresented groups are encouraged to apply. The successful candidate will become a faculty member in an interdisciplinary doctoral program in Nanoscience and Nanoengineering, with an anticipated start date of August 20, 2018. Email Steve.Smith-at-sdsmt.edu for further information regarding this position.
Individuals interested in this position must apply online at http://www.sdsmt.edu/employment. Human Resources can provide accommodation to the online application process and may be reached at (605) 394-1203. Review of applications will begin December 1, 2017, and will continue until the position is filled. Employment is contingent upon completion of a satisfactory background investigation. ------------------------------------------ Phil Ahrenkiel, PhD Nanoscience and Nanoengineering South Dakota School of Mines and Technology 501 E. Saint Joseph St. Rapid City, SD 57701 U.S.A. Office: EP 221 Phone: 605-394-5238, Cell: 720-988-6627 Phil.Ahrenkiel-at-sdsmt.edu http://ahrenkiel.sdsmt.edu/
==============================Original Headers============================== 9, 39 -- From Phil.Ahrenkiel-at-sdsmt.edu Sun Oct 22 09:55:03 2017 9, 39 -- Received: from SDSMT-email.sdsmt.edu (sdsmt-email.sdsmt.edu [151.159.3.19]) 9, 39 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v9MEt3Qp005892 9, 39 -- for {Microscopy-at-Microscopy.Com} ; Sun, 22 Oct 2017 09:55:03 -0500 9, 39 -- Received: from sdsmt-ex13.sdsmt.edu (sdsmt-ex13.sdsmt.edu [IPv6:2607:f558:1000:f:0:0:0:6d]) 9, 39 -- by SDSMT-email.sdsmt.edu (8.15.2/8.15.2) with ESMTPS id v9MEo9so001436 9, 39 -- (version=TLSv1.2 cipher=AES256-SHA bits=256 verify=OK) 9, 39 -- for {Microscopy-at-Microscopy.Com} ; Sun, 22 Oct 2017 08:50:09 -0600 9, 39 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=sdsmt.edu; 9, 39 -- s=sdsmtdkim; t=1508683809; 9, 39 -- bh=ae/AvS+ZBFy6MEglqhpMktvKwDHrcNm/CoupEYk5zpY=; 9, 39 -- h=From:To:Subject:Date; 9, 39 -- b=E3Xo9C4NrV928KNS2CIT8sm2L8w384ScBOdRvjuTBBwdHBBKD2Xt2P1pgNkHb3wc3 9, 39 -- IAuzp1rPgAIsGmfvbvP1NFXefPBsh8ks6lR4FZWQVclu5TBWROVCCFoeHEQLTQmzxO 9, 39 -- UPET4b3t/lyjaC2mnHdEgBmaaE25Yziz/dgrGxqg= 9, 39 -- Received: from SDSMT-ex13.sdsmt.edu (2607:f558:1000:f::6d) by 9, 39 -- SDSMT-ex13.sdsmt.edu (2607:f558:1000:f::6d) with Microsoft SMTP Server (TLS) 9, 39 -- id 15.0.1347.2; Sun, 22 Oct 2017 08:50:09 -0600 9, 39 -- Received: from SDSMT-ex13.sdsmt.edu ([fe80::1492:416b:26a0:40e8]) by 9, 39 -- SDSMT-ex13.sdsmt.edu ([fe80::1492:416b:26a0:40e8%15]) with mapi id 9, 39 -- 15.00.1347.000; Sun, 22 Oct 2017 08:50:09 -0600 9, 39 -- From: "Ahrenkiel, Phil" {Phil.Ahrenkiel-at-sdsmt.edu} 9, 39 -- To: "'Microscopy-at-Microscopy.Com' (Microscopy-at-Microscopy.Com)" 9, 39 -- {Microscopy-at-Microscopy.Com} 9, 39 -- Subject: Faculty Position-Nanoscience and Nanoengineering 9, 39 -- Thread-Topic: Faculty Position-Nanoscience and Nanoengineering 9, 39 -- Thread-Index: AdNLRQM37IHRDLqnQKeFbiCoFE/keQ== 9, 39 -- Date: Sun, 22 Oct 2017 14:50:08 +0000 9, 39 -- Message-ID: {56a4e091005d4bd6b758add6fe8deaec-at-SDSMT-ex13.sdsmt.edu} 9, 39 -- Accept-Language: en-US 9, 39 -- Content-Language: en-US 9, 39 -- X-MS-Has-Attach: 9, 39 -- X-MS-TNEF-Correlator: 9, 39 -- x-ms-exchange-transport-fromentityheader: Hosted 9, 39 -- x-originating-ip: [2607:f558:1000:14:8812:624b:b83d:c7fd] 9, 39 -- Content-Type: text/plain; charset="iso-8859-1" 9, 39 -- MIME-Version: 1.0 9, 39 -- Content-Transfer-Encoding: 8bit 9, 39 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v9MEt3Qp005892 ==============================End of - Headers==============================
Hi everyone, I have the opportunity to change away from individual chiller units attached to SEMs and TEMs and to work with our physical facilities to design, build and run something new. I am looking at a single system which will cover multiple microscopes. I am aware of the need for strict temperature control, no vibration, cooling and pumping redundancy etc. I wondered if anyone else had gone down this path?
Chris
Christopher J. Gilpin Ph.D. Director, Life Science Microscopy Facility Campus-wide Coordinator for Electron Microscopy Purdue University Whistler Hall of Agriculture Research, Room S052 170 S. University St West Lafayette, IN 47907 765-494-7750 gilpin-at-purdue.edu lsmf-at-purdue.edu reaches everyone in the facility. http://ag.purdue.edu/arp/Microscopy/Pages/default.aspx
==============================Original Headers============================== 7, 32 -- From gilpin-at-purdue.edu Mon Oct 23 09:43:11 2017 7, 32 -- Received: from xppmailspam04.itap.purdue.edu (xppmailspam04.itap.purdue.edu [128.210.5.15]) 7, 32 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v9NEhB1a017666 7, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Oct 2017 09:43:11 -0500 7, 32 -- X-IronPort-Anti-Spam-Filtered: true 7, 32 -- X-IronPort-AV: E=Sophos;i="5.43,423,1503374400"; 7, 32 -- d="scan'208";a="53891616" 7, 32 -- Received: from exchange.purdue.edu ([128.210.1.29]) 7, 32 -- by xppmailspam04.itap.purdue.edu with ESMTP/TLS/AES256-SHA; 23 Oct 2017 10:38:22 -0400 7, 32 -- Received: from wppexc03.purdue.lcl (172.30.136.176) by wppexc08.purdue.lcl 7, 32 -- (172.30.136.181) with Microsoft SMTP Server (TLS) id 15.0.1178.4; Mon, 23 Oct 7, 32 -- 2017 10:38:22 -0400 7, 32 -- Received: from wppexc03.purdue.lcl ([fe80::3c86:dcb8:fd99:f174]) by 7, 32 -- wppexc03.purdue.lcl ([fe80::3c86:dcb8:fd99:f174%15]) with mapi id 7, 32 -- 15.00.1178.000; Mon, 23 Oct 2017 10:38:22 -0400 7, 32 -- From: "Gilpin, Christopher J" {gilpin-at-purdue.edu} 7, 32 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 7, 32 -- Subject: novel chilled water circulator systems 7, 32 -- Thread-Topic: novel chilled water circulator systems 7, 32 -- Thread-Index: AdNMDJK6buGxezYqSHCaJWTqaxkiHw== 7, 32 -- Date: Mon, 23 Oct 2017 14:38:21 +0000 7, 32 -- Message-ID: {437c283aab224a0ca1c929b57208d9ee-at-wppexc03.purdue.lcl} 7, 32 -- Accept-Language: en-US 7, 32 -- Content-Language: en-US 7, 32 -- X-MS-Has-Attach: 7, 32 -- X-MS-TNEF-Correlator: 7, 32 -- x-ms-exchange-transport-fromentityheader: Hosted 7, 32 -- x-originating-ip: [10.163.23.200] 7, 32 -- Content-Type: text/plain; charset="utf-8" 7, 32 -- MIME-Version: 1.0 7, 32 -- Content-Transfer-Encoding: 8bit 7, 32 -- X-MIME-Autoconverted: from base64 to 8bit by microscopy.com id v9NEhB1a017666 ==============================End of - Headers==============================
Christopher; When I visited the JEOL factory, I learned that they had a large water tank on the roof to feed all of the microscopes by gravity. The only pumping was to pump the ‘used’ water back up to the tank. Because of the large size of the tank, the water temperature had good stability. There was no turbulence in the water flow, and the overall cost of the system was quite low.
A. John Mardinly, Ph.D., P.E., retired } On Oct 23, 2017, at 7:56 AM, gilpin-at-purdue.edu wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.com_MicroscopyListserver&d=DwIBaQ&c=l45AxH-kUV29SRQusp9vYR0n1GycN4_2jInuKy6zbqQ&r=VLPJ8OE-c_C6joGeE1ftlvxMmQPq9N6mpKZONBRt90E&m=LgYf1ilTFnw-UUphomi4qg7aBDAK8HkNIwYlTiToZ70&s=FcwLKuNJb5QhNE-oUANBL12FF533jOs76DS0L0S8864&e= } On-Line Help https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.com_MicroscopyListserver_FAQ.html&d=DwIBaQ&c=l45AxH-kUV29SRQusp9vYR0n1GycN4_2jInuKy6zbqQ&r=VLPJ8OE-c_C6joGeE1ftlvxMmQPq9N6mpKZONBRt90E&m=LgYf1ilTFnw-UUphomi4qg7aBDAK8HkNIwYlTiToZ70&s=EZlga-TtT8e-4o6i9BJv57FMksPNhquiK4kV9UFFpX8&e= } ---------------------------------------------------------------------------- } } } Hi everyone, } I have the opportunity to change away from individual chiller units attached to SEMs and TEMs and to work with our physical facilities to design, build and run something new. I am looking at a single system which will cover multiple microscopes. I am aware of the need for strict temperature control, no vibration, cooling and pumping redundancy etc. I wondered if anyone else had gone down this path? } } } Chris } } Christopher J. Gilpin Ph.D. } Director, Life Science Microscopy Facility } Campus-wide Coordinator for Electron Microscopy } Purdue University } Whistler Hall of Agriculture Research, Room S052 } 170 S. University St } West Lafayette, IN 47907 } 765-494-7750 } gilpin-at-purdue.edu } lsmf-at-purdue.edu reaches everyone in the facility. } https://urldefense.proofpoint.com/v2/url?u=http-3A__ag.purdue.edu_arp_Microscopy_Pages_default.aspx&d=DwIBaQ&c=l45AxH-kUV29SRQusp9vYR0n1GycN4_2jInuKy6zbqQ&r=VLPJ8OE-c_C6joGeE1ftlvxMmQPq9N6mpKZONBRt90E&m=LgYf1ilTFnw-UUphomi4qg7aBDAK8HkNIwYlTiToZ70&s=13aOqumsN3kWhQfnTCtdnyC16JGs89Hoo4O8P4LTkvs&e= } } } } } ==============================Original Headers============================== } 7, 32 -- From gilpin-at-purdue.edu Mon Oct 23 09:43:11 2017 } 7, 32 -- Received: from xppmailspam04.itap.purdue.edu (xppmailspam04.itap.purdue.edu [128.210.5.15]) } 7, 32 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v9NEhB1a017666 } 7, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Oct 2017 09:43:11 -0500 } 7, 32 -- X-IronPort-Anti-Spam-Filtered: true } 7, 32 -- X-IronPort-AV: E=Sophos;i="5.43,423,1503374400"; } 7, 32 -- d="scan'208";a="53891616" } 7, 32 -- Received: from exchange.purdue.edu ([128.210.1.29]) } 7, 32 -- by xppmailspam04.itap.purdue.edu with ESMTP/TLS/AES256-SHA; 23 Oct 2017 10:38:22 -0400 } 7, 32 -- Received: from wppexc03.purdue.lcl (172.30.136.176) by wppexc08.purdue.lcl } 7, 32 -- (172.30.136.181) with Microsoft SMTP Server (TLS) id 15.0.1178.4; Mon, 23 Oct } 7, 32 -- 2017 10:38:22 -0400 } 7, 32 -- Received: from wppexc03.purdue.lcl ([fe80::3c86:dcb8:fd99:f174]) by } 7, 32 -- wppexc03.purdue.lcl ([fe80::3c86:dcb8:fd99:f174%15]) with mapi id } 7, 32 -- 15.00.1178.000; Mon, 23 Oct 2017 10:38:22 -0400 } 7, 32 -- From: "Gilpin, Christopher J" {gilpin-at-purdue.edu} } 7, 32 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} } 7, 32 -- Subject: novel chilled water circulator systems } 7, 32 -- Thread-Topic: novel chilled water circulator systems } 7, 32 -- Thread-Index: AdNMDJK6buGxezYqSHCaJWTqaxkiHw== } 7, 32 -- Date: Mon, 23 Oct 2017 14:38:21 +0000 } 7, 32 -- Message-ID: {437c283aab224a0ca1c929b57208d9ee-at-wppexc03.purdue.lcl} } 7, 32 -- Accept-Language: en-US } 7, 32 -- Content-Language: en-US } 7, 32 -- X-MS-Has-Attach: } 7, 32 -- X-MS-TNEF-Correlator: } 7, 32 -- x-ms-exchange-transport-fromentityheader: Hosted } 7, 32 -- x-originating-ip: [10.163.23.200] } 7, 32 -- Content-Type: text/plain; charset="utf-8" } 7, 32 -- MIME-Version: 1.0 } 7, 32 -- Content-Transfer-Encoding: 8bit } 7, 32 -- X-MIME-Autoconverted: from base64 to 8bit by microscopy.com id v9NEhB1a017666 } ==============================End of - Headers==============================
We have a building chiller that supplies chilled water for the building temperature control and for heat exchangers used on the microscopes to give us the temperature stability we need. Unless it is oversized you will not get the temperature stability you require from a building chiller (ours has a 5 degree F range). Ours is outside so needs to be a 30% glycol/water mixture to survive the midwest winters.
This has worked reasonably well for us since 1998, however if (or rather when) the building chiller goes down all of the microscopes go down as well.
Alan
On 10/23/2017 9:44 AM, gilpin-at-purdue.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } Hi everyone, } I have the opportunity to change away from individual chiller units attached to SEMs and TEMs and to work with our physical facilities to design, build and run something new. I am looking at a single system which will cover multiple microscopes. I am aware of the need for strict temperature control, no vibration, cooling and pumping redundancy etc. I wondered if anyone else had gone down this path? } } ​​​​​ } Chris } } Christopher J. Gilpin Ph.D. } Director, Life Science Microscopy Facility } Campus-wide Coordinator for Electron Microscopy } Purdue University } Whistler Hall of Agriculture Research, Room S052 } 170 S. University St } West Lafayette, IN 47907 } 765-494-7750 } gilpin-at-purdue.edu } lsmf-at-purdue.edu reaches everyone in the facility. } http://ag.purdue.edu/arp/Microscopy/Pages/default.aspx } } } } } ==============================Original Headers============================== } 7, 32 -- From gilpin-at-purdue.edu Mon Oct 23 09:43:11 2017 } 7, 32 -- Received: from xppmailspam04.itap.purdue.edu (xppmailspam04.itap.purdue.edu [128.210.5.15]) } 7, 32 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v9NEhB1a017666 } 7, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Oct 2017 09:43:11 -0500 } 7, 32 -- X-IronPort-Anti-Spam-Filtered: true } 7, 32 -- X-IronPort-AV: E=Sophos;i="5.43,423,1503374400"; } 7, 32 -- d="scan'208";a="53891616" } 7, 32 -- Received: from exchange.purdue.edu ([128.210.1.29]) } 7, 32 -- by xppmailspam04.itap.purdue.edu with ESMTP/TLS/AES256-SHA; 23 Oct 2017 10:38:22 -0400 } 7, 32 -- Received: from wppexc03.purdue.lcl (172.30.136.176) by wppexc08.purdue.lcl } 7, 32 -- (172.30.136.181) with Microsoft SMTP Server (TLS) id 15.0.1178.4; Mon, 23 Oct } 7, 32 -- 2017 10:38:22 -0400 } 7, 32 -- Received: from wppexc03.purdue.lcl ([fe80::3c86:dcb8:fd99:f174]) by } 7, 32 -- wppexc03.purdue.lcl ([fe80::3c86:dcb8:fd99:f174%15]) with mapi id } 7, 32 -- 15.00.1178.000; Mon, 23 Oct 2017 10:38:22 -0400 } 7, 32 -- From: "Gilpin, Christopher J" {gilpin-at-purdue.edu} } 7, 32 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} } 7, 32 -- Subject: novel chilled water circulator systems } 7, 32 -- Thread-Topic: novel chilled water circulator systems } 7, 32 -- Thread-Index: AdNMDJK6buGxezYqSHCaJWTqaxkiHw== } 7, 32 -- Date: Mon, 23 Oct 2017 14:38:21 +0000 } 7, 32 -- Message-ID: {437c283aab224a0ca1c929b57208d9ee-at-wppexc03.purdue.lcl} } 7, 32 -- Accept-Language: en-US } 7, 32 -- Content-Language: en-US } 7, 32 -- X-MS-Has-Attach: } 7, 32 -- X-MS-TNEF-Correlator: } 7, 32 -- x-ms-exchange-transport-fromentityheader: Hosted } 7, 32 -- x-originating-ip: [10.163.23.200] } 7, 32 -- Content-Type: text/plain; charset="utf-8" } 7, 32 -- MIME-Version: 1.0 } 7, 32 -- Content-Transfer-Encoding: 8bit } 7, 32 -- X-MIME-Autoconverted: from base64 to 8bit by microscopy.com id v9NEhB1a017666 } ==============================End of - Headers============================== } }
-- Alan W Nicholls, PhD Associate Director, RRC Director of Research Service Facility (Electron Microscopy) Research Resources Center - East (M/C 337) Room 110, Science and Engineering South Building The University of Illinois at Chicago 845 West Taylor St Chicago, IL 60607-7058
Tel: 312 996 1227
Web site http://www.rrc.uic.edu/ems Wiki site https://wiki.rrc.uic.edu/wiki/EMS
==============================Original Headers============================== 9, 22 -- From nicholls-at-uic.edu Mon Oct 23 11:46:15 2017 9, 22 -- Received: from mail-1.cc.uic.edu (mail-1-456.cc.uic.edu [128.248.156.182]) 9, 22 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v9NGkFjp031513 9, 22 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Oct 2017 11:46:15 -0500 9, 22 -- Received: from [131.193.156.230] (p30633.gh306.uic.edu [131.193.156.230]) 9, 22 -- (authenticated bits=0) 9, 22 -- by mail-1.cc.uic.edu (8.14.4/8.14.4) with ESMTP id v9NGfQQp004451 9, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES128-SHA bits=128 verify=NOT); 9, 22 -- Mon, 23 Oct 2017 11:41:26 -0500 9, 22 -- Subject: Re: [Microscopy] novel chilled water circulator systems 9, 22 -- To: gilpin-at-purdue.edu, Microscopy-at-microscopy.com 9, 22 -- References: {201710231444.v9NEi5hq018514-at-microscopy.com} 9, 22 -- From: Alan Nicholls {nicholls-at-uic.edu} 9, 22 -- Message-ID: {0dc15938-fe57-4de5-947a-0f875d11aa0d-at-uic.edu} 9, 22 -- Date: Mon, 23 Oct 2017 11:41:27 -0500 9, 22 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:52.0) Gecko/20100101 9, 22 -- Thunderbird/52.4.0 9, 22 -- MIME-Version: 1.0 9, 22 -- In-Reply-To: {201710231444.v9NEi5hq018514-at-microscopy.com} 9, 22 -- Content-Type: text/plain; charset=utf-8; format=flowed 9, 22 -- Content-Transfer-Encoding: 8bit 9, 22 -- Content-Language: en-US ==============================End of - Headers==============================
This is exactly the type of system I am trying to set up in our building- do you have any more information you can share on the heat exchanger, and how the regulation of temperature is achieved?
Best regards,
Ben
-- Research Support Manager (Imaging and IT Systems) MRC Brain Network Dynamics Unit, University of Oxford Department of Pharmacology, Mansfield Road, Oxford, OX1 3TH, United Kingdom. Telephone: 01865 271897 {http://www.mrcbndu.ox.ac.uk/}
-----Original Message----- X-from: nicholls-at-uic.edu [mailto:nicholls-at-uic.edu] Sent: 23 October 2017 17:56
Hi Chris,
We have such a "shared" cooling system in our CAMCOR facility that supports around 10 beam instruments.
http://camcor.uoregon.edu/
We still have each instrument on its own dedicated water cooled chiller but each chiller is cooled by the shared cooling system. That way we don't have huge air cooling requirements. There is a short discussion of cooling systems here:
On 10/23/2017 7:49 AM, gilpin-at-purdue.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } Hi everyone, } I have the opportunity to change away from individual chiller units attached to SEMs and TEMs and to work with our physical facilities to design, build and run something new. I am looking at a single system which will cover multiple microscopes. I am aware of the need for strict temperature control, no vibration, cooling and pumping redundancy etc. I wondered if anyone else had gone down this path? } } ​​​​​ } Chris } } Christopher J. Gilpin Ph.D. } Director, Life Science Microscopy Facility } Campus-wide Coordinator for Electron Microscopy } Purdue University } Whistler Hall of Agriculture Research, Room S052 } 170 S. University St } West Lafayette, IN 47907 } 765-494-7750 } gilpin-at-purdue.edu } lsmf-at-purdue.edu reaches everyone in the facility. } http://ag.purdue.edu/arp/Microscopy/Pages/default.aspx } } } } } ==============================Original Headers============================== } 7, 32 -- From gilpin-at-purdue.edu Mon Oct 23 09:43:11 2017 } 7, 32 -- Received: from xppmailspam04.itap.purdue.edu (xppmailspam04.itap.purdue.edu [128.210.5.15]) } 7, 32 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v9NEhB1a017666 } 7, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Oct 2017 09:43:11 -0500 } 7, 32 -- X-IronPort-Anti-Spam-Filtered: true } 7, 32 -- X-IronPort-AV: E=Sophos;i="5.43,423,1503374400"; } 7, 32 -- d="scan'208";a="53891616" } 7, 32 -- Received: from exchange.purdue.edu ([128.210.1.29]) } 7, 32 -- by xppmailspam04.itap.purdue.edu with ESMTP/TLS/AES256-SHA; 23 Oct 2017 10:38:22 -0400 } 7, 32 -- Received: from wppexc03.purdue.lcl (172.30.136.176) by wppexc08.purdue.lcl } 7, 32 -- (172.30.136.181) with Microsoft SMTP Server (TLS) id 15.0.1178.4; Mon, 23 Oct } 7, 32 -- 2017 10:38:22 -0400 } 7, 32 -- Received: from wppexc03.purdue.lcl ([fe80::3c86:dcb8:fd99:f174]) by } 7, 32 -- wppexc03.purdue.lcl ([fe80::3c86:dcb8:fd99:f174%15]) with mapi id } 7, 32 -- 15.00.1178.000; Mon, 23 Oct 2017 10:38:22 -0400 } 7, 32 -- From: "Gilpin, Christopher J" {gilpin-at-purdue.edu} } 7, 32 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} } 7, 32 -- Subject: novel chilled water circulator systems } 7, 32 -- Thread-Topic: novel chilled water circulator systems } 7, 32 -- Thread-Index: AdNMDJK6buGxezYqSHCaJWTqaxkiHw== } 7, 32 -- Date: Mon, 23 Oct 2017 14:38:21 +0000 } 7, 32 -- Message-ID: {437c283aab224a0ca1c929b57208d9ee-at-wppexc03.purdue.lcl} } 7, 32 -- Accept-Language: en-US } 7, 32 -- Content-Language: en-US } 7, 32 -- X-MS-Has-Attach: } 7, 32 -- X-MS-TNEF-Correlator: } 7, 32 -- x-ms-exchange-transport-fromentityheader: Hosted } 7, 32 -- x-originating-ip: [10.163.23.200] } 7, 32 -- Content-Type: text/plain; charset="utf-8" } 7, 32 -- MIME-Version: 1.0 } 7, 32 -- Content-Transfer-Encoding: 8bit } 7, 32 -- X-MIME-Autoconverted: from base64 to 8bit by microscopy.com id v9NEhB1a017666 } ==============================End of - Headers==============================
-- John J. Donovan donovan-at-uoregon.edu University of Oregon (541) 346-4632 (office) 1443 E. 13th Ave (541) 346-4655 (probe) Eugene, OR (541) 346-6854 (FAX) 97403-1241
For those of you interested in a centralized chiller system, our company can design and deliver an application specific unit that will provide temperature control and redundancy. Please contact me directly if you would like to find out more.
On Mon, Oct 23, 2017 at 1:25 PM, {ben.micklem-at-pharm.ox.ac.uk} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Alan, } } This is exactly the type of system I am trying to set up in our building- do you have any more information you can share on the heat exchanger, and how the regulation of temperature is achieved? } } Best regards, } } Ben } } } -- } Research Support Manager (Imaging and IT Systems) } MRC Brain Network Dynamics Unit, } University of Oxford Department of Pharmacology, } Mansfield Road, Oxford, OX1 3TH, United Kingdom. } Telephone: 01865 271897 {http://www.mrcbndu.ox.ac.uk/} } } } } -----Original Message----- } X-from: nicholls-at-uic.edu [mailto:nicholls-at-uic.edu] } Sent: 23 October 2017 17:56 } Subject: [Microscopy] Re: novel chilled water circulator systems } } Chris } } We have a building chiller that supplies chilled water for the building } temperature control and for heat exchangers used on the microscopes to } give us the temperature stability we need. Unless it is oversized you } will not get the temperature stability you require from a building } chiller (ours has a 5 degree F range). Ours is outside so needs to be a } 30% glycol/water mixture to survive the midwest winters. } } This has worked reasonably well for us since 1998, however if (or rather } when) the building chiller goes down all of the microscopes go down as well. } } Alan } } } } ==============================Original Headers============================== } 15, 34 -- From ben.micklem-at-pharm.ox.ac.uk Mon Oct 23 12:02:42 2017 } 15, 34 -- Received: from relay14.mail.ox.ac.uk (relay14.mail.ox.ac.uk [163.1.2.162]) } 15, 34 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v9NH2fO5014926 } 15, 34 -- for {microscopy-at-microscopy.com} ; Mon, 23 Oct 2017 12:02:42 -0500 } 15, 34 -- Received: from hub05.nexus.ox.ac.uk ([163.1.154.231] helo=HUB05.ad.oak.ox.ac.uk) } 15, 34 -- by relay14.mail.ox.ac.uk with esmtps (TLS1.0:ECDHE_RSA_AES_256_CBC_SHA1:256) } 15, 34 -- (Exim 4.89) } 15, 34 -- (envelope-from {ben.micklem-at-pharm.ox.ac.uk} ) } 15, 34 -- id 1e6g2q-0006fn-lO; Mon, 23 Oct 2017 17:57:53 +0100 } 15, 34 -- Received: from MBX03.ad.oak.ox.ac.uk ([169.254.3.200]) by } 15, 34 -- HUB05.ad.oak.ox.ac.uk ([163.1.154.96]) with mapi id 14.03.0248.002; Mon, 23 } 15, 34 -- Oct 2017 17:56:20 +0100 } 15, 34 -- From: Ben Micklem {ben.micklem-at-pharm.ox.ac.uk} } 15, 34 -- To: "nicholls-at-uic.edu" {nicholls-at-uic.edu} , } 15, 34 -- "microscopy-at-microscopy.com" } 15, 34 -- {microscopy-at-microscopy.com} } 15, 34 -- Subject: RE: [Microscopy] Re: novel chilled water circulator systems } 15, 34 -- Thread-Topic: [Microscopy] Re: novel chilled water circulator systems } 15, 34 -- Thread-Index: AQHTTB8yTNU60b812k6rdFMuVIX1n6Lxpv8Q } 15, 34 -- Date: Mon, 23 Oct 2017 16:56:18 +0000 } 15, 34 -- Message-ID: {168A63C1140FEB4EB61870A513446A4A18A84238-at-MBX03.ad.oak.ox.ac.uk} } 15, 34 -- References: {201710231656.v9NGuNJl008337-at-microscopy.com} } 15, 34 -- In-Reply-To: {201710231656.v9NGuNJl008337-at-microscopy.com} } 15, 34 -- Accept-Language: en-GB, en-US } 15, 34 -- Content-Language: en-US } 15, 34 -- X-MS-Has-Attach: } 15, 34 -- X-MS-TNEF-Correlator: } 15, 34 -- x-originating-ip: [172.16.150.238] } 15, 34 -- Content-Type: text/plain; charset="utf-8" } 15, 34 -- MIME-Version: 1.0 } 15, 34 -- X-Oxmail-Spam-Status: score=0.0 tests=none } 15, 34 -- X-Oxmail-Spam-Level: / } 15, 34 -- Content-Transfer-Encoding: 8bit } 15, 34 -- X-MIME-Autoconverted: from base64 to 8bit by microscopy.com id v9NH2fO5014926 } ==============================End of - Headers==============================
-- Thank You, Douglas D. Roffle
U.S. 716-592-4402 | FRANCE +330975181013 | FAX 716-592-4407 W jkraftmicro.com | A 243 W Main St | PO Box 386 | Springville, NY 14141
The units were supplied by Haskris (Model WW2 - water to water, non-refrigerated heat exchanger). They all have electronic close temperature control which has an immersion heater which is used with a modulating water control valve. The modulating valve varies the flow of secondary cooling water to maintain a relatively constant supply water temperature. The electronically controlled immersion heater switches on and off to compensate for any remaining fluctuations in the supply water temperature which results in extremely close temperature control.
Regards
Alan
On 10/23/2017 11:56 AM, Ben Micklem wrote: } Dear Alan, } } This is exactly the type of system I am trying to set up in our building- do you have any more information you can share on the heat exchanger, and how the regulation of temperature is achieved? } } Best regards, } } Ben } } } -- } Research Support Manager (Imaging and IT Systems) } MRC Brain Network Dynamics Unit, } University of Oxford Department of Pharmacology, } Mansfield Road, Oxford, OX1 3TH, United Kingdom. } Telephone: 01865 271897 {http://www.mrcbndu.ox.ac.uk/} } } } } -----Original Message----- } From: nicholls-at-uic.edu [mailto:nicholls-at-uic.edu] } Sent: 23 October 2017 17:56 } Subject: [Microscopy] Re: novel chilled water circulator systems } } Chris } } We have a building chiller that supplies chilled water for the building } temperature control and for heat exchangers used on the microscopes to } give us the temperature stability we need. Unless it is oversized you } will not get the temperature stability you require from a building } chiller (ours has a 5 degree F range). Ours is outside so needs to be a } 30% glycol/water mixture to survive the midwest winters. } } This has worked reasonably well for us since 1998, however if (or rather } when) the building chiller goes down all of the microscopes go down as well. } } Alan }
-- Alan W Nicholls, PhD Associate Director, RRC Director of Research Service Facility (Electron Microscopy) Research Resources Center - East (M/C 337) Room 110, Science and Engineering South Building The University of Illinois at Chicago 845 West Taylor St Chicago, IL 60607-7058
Tel: 312 996 1227
Web site http://www.rrc.uic.edu/ems Wiki site https://wiki.rrc.uic.edu/wiki/EMS
==============================Original Headers============================== 9, 24 -- From nicholls-at-uic.edu Mon Oct 23 13:53:23 2017 9, 24 -- Received: from mail-1.cc.uic.edu (mail-1-456.cc.uic.edu [128.248.156.182]) 9, 24 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v9NIrNbO025432 9, 24 -- for {microscopy-at-microscopy.com} ; Mon, 23 Oct 2017 13:53:23 -0500 9, 24 -- Received: from [131.193.156.230] (p30633.gh306.uic.edu [131.193.156.230]) 9, 24 -- (authenticated bits=0) 9, 24 -- by mail-1.cc.uic.edu (8.14.4/8.14.4) with ESMTP id v9NImYt9016544 9, 24 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES128-SHA bits=128 verify=NOT); 9, 24 -- Mon, 23 Oct 2017 13:48:34 -0500 9, 24 -- Subject: Re: [Microscopy] Re: novel chilled water circulator systems 9, 24 -- To: Ben Micklem {ben.micklem-at-pharm.ox.ac.uk} , 9, 24 -- "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 9, 24 -- References: {201710231656.v9NGuNJl008337-at-microscopy.com} 9, 24 -- {168A63C1140FEB4EB61870A513446A4A18A84238-at-MBX03.ad.oak.ox.ac.uk} 9, 24 -- From: Alan Nicholls {nicholls-at-uic.edu} 9, 24 -- Message-ID: {e493668d-f034-581b-79b3-1ebcc6075e62-at-uic.edu} 9, 24 -- Date: Mon, 23 Oct 2017 13:48:35 -0500 9, 24 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:52.0) Gecko/20100101 9, 24 -- Thunderbird/52.4.0 9, 24 -- MIME-Version: 1.0 9, 24 -- In-Reply-To: {168A63C1140FEB4EB61870A513446A4A18A84238-at-MBX03.ad.oak.ox.ac.uk} 9, 24 -- Content-Type: text/plain; charset=utf-8; format=flowed 9, 24 -- Content-Transfer-Encoding: 8bit 9, 24 -- Content-Language: en-US ==============================End of - Headers==============================
The units were supplied by Haskris (Model WW2 - water to water, non-refrigerated heat exchanger). They all have electronic close temperature control which has an immersion heater which is used with a modulating water control valve. The modulating valve varies the flow of secondary cooling water to maintain a relatively constant supply water temperature. The electronically controlled immersion heater switches on and off to compensate for any remaining fluctuations in the supply water temperature which results in extremely close temperature control.
Regards
Alan
On 10/23/2017 11:56 AM, Ben Micklem wrote: } Dear Alan, } } This is exactly the type of system I am trying to set up in our building- do you have any more information you can share on the heat exchanger, and how the regulation of temperature is achieved? } } Best regards, } } Ben } } } -- } Research Support Manager (Imaging and IT Systems) } MRC Brain Network Dynamics Unit, } University of Oxford Department of Pharmacology, } Mansfield Road, Oxford, OX1 3TH, United Kingdom. } Telephone: 01865 271897 {http://www.mrcbndu.ox.ac.uk/} } } } } -----Original Message----- } From: nicholls-at-uic.edu [mailto:nicholls-at-uic.edu] } Sent: 23 October 2017 17:56 } Subject: [Microscopy] Re: novel chilled water circulator systems } } Chris } } We have a building chiller that supplies chilled water for the building } temperature control and for heat exchangers used on the microscopes to } give us the temperature stability we need. Unless it is oversized you } will not get the temperature stability you require from a building } chiller (ours has a 5 degree F range). Ours is outside so needs to be a } 30% glycol/water mixture to survive the midwest winters. } } This has worked reasonably well for us since 1998, however if (or rather } when) the building chiller goes down all of the microscopes go down as well. } } Alan }
-- Alan W Nicholls, PhD Associate Director, RRC Director of Research Service Facility (Electron Microscopy) Research Resources Center - East (M/C 337) Room 110, Science and Engineering South Building The University of Illinois at Chicago 845 West Taylor St Chicago, IL 60607-7058
Tel: 312 996 1227
Web site http://www.rrc.uic.edu/ems Wiki site https://wiki.rrc.uic.edu/wiki/EMS
==============================Original Headers============================== 9, 24 -- From nicholls-at-uic.edu Mon Oct 23 13:53:23 2017 9, 24 -- Received: from mail-1.cc.uic.edu (mail-1-456.cc.uic.edu [128.248.156.182]) 9, 24 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v9NIrNbO025432 9, 24 -- for {microscopy-at-microscopy.com} ; Mon, 23 Oct 2017 13:53:23 -0500 9, 24 -- Received: from [131.193.156.230] (p30633.gh306.uic.edu [131.193.156.230]) 9, 24 -- (authenticated bits=0) 9, 24 -- by mail-1.cc.uic.edu (8.14.4/8.14.4) with ESMTP id v9NImYt9016544 9, 24 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES128-SHA bits=128 verify=NOT); 9, 24 -- Mon, 23 Oct 2017 13:48:34 -0500 9, 24 -- Subject: Re: [Microscopy] Re: novel chilled water circulator systems 9, 24 -- To: Ben Micklem {ben.micklem-at-pharm.ox.ac.uk} , 9, 24 -- "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 9, 24 -- References: {201710231656.v9NGuNJl008337-at-microscopy.com} 9, 24 -- {168A63C1140FEB4EB61870A513446A4A18A84238-at-MBX03.ad.oak.ox.ac.uk} 9, 24 -- From: Alan Nicholls {nicholls-at-uic.edu} 9, 24 -- Message-ID: {e493668d-f034-581b-79b3-1ebcc6075e62-at-uic.edu} 9, 24 -- Date: Mon, 23 Oct 2017 13:48:35 -0500 9, 24 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:52.0) Gecko/20100101 9, 24 -- Thunderbird/52.4.0 9, 24 -- MIME-Version: 1.0 9, 24 -- In-Reply-To: {168A63C1140FEB4EB61870A513446A4A18A84238-at-MBX03.ad.oak.ox.ac.uk} 9, 24 -- Content-Type: text/plain; charset=utf-8; format=flowed 9, 24 -- Content-Transfer-Encoding: 8bit 9, 24 -- Content-Language: en-US ==============================End of - Headers==============================
==============================Original Headers============================== 11, 22 -- From nicholls-at-uic.edu Mon Oct 23 14:28:06 2017 11, 22 -- Received: from mail-1.cc.uic.edu (mail-1-456.cc.uic.edu [128.248.156.182]) 11, 22 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v9NJS6Hi015729 11, 22 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Oct 2017 14:28:06 -0500 11, 22 -- Received: from [131.193.156.230] (p30633.gh306.uic.edu [131.193.156.230]) 11, 22 -- (authenticated bits=0) 11, 22 -- by mail-1.cc.uic.edu (8.14.4/8.14.4) with ESMTP id v9NJNI1T003532 11, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES128-SHA bits=128 verify=NOT); 11, 22 -- Mon, 23 Oct 2017 14:23:18 -0500 11, 22 -- From: Alan Nicholls {nicholls-at-uic.edu} 11, 22 -- Subject: [Microscopy] novel chilled water circulator systems 11, 22 -- Reply-To: nicholls-at-uic.edu 11, 22 -- To: Ben Micklem {ben.micklem-at-pharm.ox.ac.uk} 11, 22 -- Cc: "microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 11, 22 -- Message-ID: {2b8cf150-aede-92d9-d4cd-d5e3d4e705c9-at-uic.edu} 11, 22 -- Date: Mon, 23 Oct 2017 14:23:19 -0500 11, 22 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:52.0) Gecko/20100101 11, 22 -- Thunderbird/52.4.0 11, 22 -- MIME-Version: 1.0 11, 22 -- Content-Type: text/plain; charset=windows-1252; format=flowed 11, 22 -- Content-Language: en-US 11, 22 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both bonnie_salyer-at-tedpella.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Message: Ted Pella, Inc. has new product lines coming and is seeking a Material Science Product Specialist to help us continue to grow. Candidates must have BS in Material Science or related, with a minimum of 5 years SEM and 2 years TEM, beyond academic environment. EDX and WDS experience desired, AFM experience a plus. Experience in semi-conductor industry is preferred, experience in forensic science field is a plus. Contact bonnie_salyer-at-tedpella.com for complete job posting or to submit resume. www.tedpella.com Spread the good word. Login Host: 12.7.209.242 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
X-from: Gary Laevsky {glaevsky.lists-at-gmail.com}
Chris
Don't do this. We tried it at ANL, had a special commerical high capacity unit designed and built to run all the instruments.
Individual units on each instrument, all of them cooled by a central cooling system is better.
When your SINGLE cooling water system goes down (and it will) then all instruments are dead in the water. However, if the central chiller which services the small units goes down, it can still be replaced by a emergency house water line. Since the house water line is simply taking away the heat from the regulated individual units (all of which have their own controllers) you can keep all the instruments running.
This also holds true for routine servicing. If you need to change a filter on your water system, all instruments must be shut down!
It was, in my opinion, our only mistake in SAMMLab and we now essentially use that large single system to cool individual units, which is huge overkill.
Two other points:
Contamination of the cooling water from all instruments (new and old) gets mixed in the large single system. If one instrument has a problem and contaminates the water then all instruments get contaminated.
Not all instruments run best at the "same temperature". We have had to tune the water temperature of some units to minimize drift and that temperature is a few degree's different than the other microscopes. This won't likely be an issue with SEM's, but it can be for HREM/AEM's.
Nestor Your Friendly Neighborhood SysOp
On 10/23/17 10:03 AM CDT, gilpin-at-purdue.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } Hi everyone, } I have the opportunity to change away from individual chiller units attached to SEMs and TEMs and to work with our physical facilities to design, build and run something new. I am looking at a single system which will cover multiple microscopes. I am aware of the need for strict temperature control, no vibration, cooling and pumping redundancy etc. I wondered if anyone else had gone down this path? } } ​​​​​ } Chris } } Christopher J. Gilpin Ph.D. } Director, Life Science Microscopy Facility } Campus-wide Coordinator for Electron Microscopy } Purdue University } Whistler Hall of Agriculture Research, Room S052 } 170 S. University St } West Lafayette, IN 47907 } 765-494-7750 } gilpin-at-purdue.edu } lsmf-at-purdue.edu reaches everyone in the facility. } http://ag.purdue.edu/arp/Microscopy/Pages/default.aspx } } } } } ==============================Original Headers============================== } 7, 32 -- From gilpin-at-purdue.edu Mon Oct 23 09:43:11 2017 } 7, 32 -- Received: from xppmailspam04.itap.purdue.edu (xppmailspam04.itap.purdue.edu [128.210.5.15]) } 7, 32 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v9NEhB1a017666 } 7, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Oct 2017 09:43:11 -0500 } 7, 32 -- X-IronPort-Anti-Spam-Filtered: true } 7, 32 -- X-IronPort-AV: E=Sophos;i="5.43,423,1503374400"; } 7, 32 -- d="scan'208";a="53891616" } 7, 32 -- Received: from exchange.purdue.edu ([128.210.1.29]) } 7, 32 -- by xppmailspam04.itap.purdue.edu with ESMTP/TLS/AES256-SHA; 23 Oct 2017 10:38:22 -0400 } 7, 32 -- Received: from wppexc03.purdue.lcl (172.30.136.176) by wppexc08.purdue.lcl } 7, 32 -- (172.30.136.181) with Microsoft SMTP Server (TLS) id 15.0.1178.4; Mon, 23 Oct } 7, 32 -- 2017 10:38:22 -0400 } 7, 32 -- Received: from wppexc03.purdue.lcl ([fe80::3c86:dcb8:fd99:f174]) by } 7, 32 -- wppexc03.purdue.lcl ([fe80::3c86:dcb8:fd99:f174%15]) with mapi id } 7, 32 -- 15.00.1178.000; Mon, 23 Oct 2017 10:38:22 -0400 } 7, 32 -- From: "Gilpin, Christopher J" {gilpin-at-purdue.edu} } 7, 32 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} } 7, 32 -- Subject: novel chilled water circulator systems } 7, 32 -- Thread-Topic: novel chilled water circulator systems } 7, 32 -- Thread-Index: AdNMDJK6buGxezYqSHCaJWTqaxkiHw== } 7, 32 -- Date: Mon, 23 Oct 2017 14:38:21 +0000 } 7, 32 -- Message-ID: {437c283aab224a0ca1c929b57208d9ee-at-wppexc03.purdue.lcl} } 7, 32 -- Accept-Language: en-US } 7, 32 -- Content-Language: en-US } 7, 32 -- X-MS-Has-Attach: } 7, 32 -- X-MS-TNEF-Correlator: } 7, 32 -- x-ms-exchange-transport-fromentityheader: Hosted } 7, 32 -- x-originating-ip: [10.163.23.200] } 7, 32 -- Content-Type: text/plain; charset="utf-8" } 7, 32 -- MIME-Version: 1.0 } 7, 32 -- Content-Transfer-Encoding: 8bit } 7, 32 -- X-MIME-Autoconverted: from base64 to 8bit by microscopy.com id v9NEhB1a017666 } ==============================End of - Headers============================== }
Nestor; I second this. ASU had a building-wide recirculation system to separate the chillers from the campus chilled water. When it went down, and it did multiple times, every microscope in the building went down. All FEG’s, Real pain.
A. John Mardinly, Ph.D., P.E., Retired, ASU Now, Classical Guitarist/Lutenist YouTube Channel: https://www.youtube.com/user/jmardinly/videos
} On Oct 24, 2017, at 4:30 AM, microscopy.listserver-at-gmail.com wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.com_MicroscopyListserver&d=DwIBaQ&c=l45AxH-kUV29SRQusp9vYR0n1GycN4_2jInuKy6zbqQ&r=VLPJ8OE-c_C6joGeE1ftlvxMmQPq9N6mpKZONBRt90E&m=csytqarbd0icv1ZERyabHXpB9dtx1JfpHEGZ6SNqRJg&s=WMMb0rfSW2CoRcZfE2GM4GY1BJo8IjZu8E8Sz4BgcqM&e= } On-Line Help https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.com_MicroscopyListserver_FAQ.html&d=DwIBaQ&c=l45AxH-kUV29SRQusp9vYR0n1GycN4_2jInuKy6zbqQ&r=VLPJ8OE-c_C6joGeE1ftlvxMmQPq9N6mpKZONBRt90E&m=csytqarbd0icv1ZERyabHXpB9dtx1JfpHEGZ6SNqRJg&s=x0UuIwETV0Wlq29x-c5aUH_CP1ziTKuyA6EPqWn7_uE&e= } ---------------------------------------------------------------------------- } } Chris } } Don't do this. We tried it at ANL, had a special commerical high capacity unit designed and built to } run all the instruments. } } Individual units on each instrument, all of them cooled by a central cooling system is better. } } } When your SINGLE cooling water system goes down (and it will) then all instruments } are dead in the water. However, if the central chiller which services the small units goes down, } it can still be replaced by a emergency house water line. Since the house water line is simply } taking away the heat from the regulated individual units (all of which have their own controllers) } you can keep all the instruments running. } } This also holds true for routine servicing. If you need to change a filter on your water system, } all instruments must be shut down! } } It was, in my opinion, our only mistake in SAMMLab and we now essentially use that large single } system to cool individual units, which is huge overkill. } } Two other points: } } Contamination of the cooling water from all instruments (new and old) gets mixed in the large } single system. If one instrument has a problem and contaminates the water then all instruments } get contaminated. } } Not all instruments run best at the "same temperature". We have had to tune the water temperature of } some units to minimize drift and that temperature is a few degree's different than the other } microscopes. This won't likely be an issue with SEM's, but it can be for HREM/AEM's. } } Nestor } Your Friendly Neighborhood SysOp } } } } On 10/23/17 10:03 AM CDT, gilpin-at-purdue.edu wrote: } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.com_MicroscopyListserver&d=DwIBaQ&c=l45AxH-kUV29SRQusp9vYR0n1GycN4_2jInuKy6zbqQ&r=VLPJ8OE-c_C6joGeE1ftlvxMmQPq9N6mpKZONBRt90E&m=csytqarbd0icv1ZERyabHXpB9dtx1JfpHEGZ6SNqRJg&s=WMMb0rfSW2CoRcZfE2GM4GY1BJo8IjZu8E8Sz4BgcqM&e= } } On-Line Help https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.com_MicroscopyListserver_FAQ.html&d=DwIBaQ&c=l45AxH-kUV29SRQusp9vYR0n1GycN4_2jInuKy6zbqQ&r=VLPJ8OE-c_C6joGeE1ftlvxMmQPq9N6mpKZONBRt90E&m=csytqarbd0icv1ZERyabHXpB9dtx1JfpHEGZ6SNqRJg&s=x0UuIwETV0Wlq29x-c5aUH_CP1ziTKuyA6EPqWn7_uE&e= } } ---------------------------------------------------------------------------- } } } } } } Hi everyone, } } I have the opportunity to change away from individual chiller units attached to SEMs and TEMs and to work with our physical facilities to design, build and run something new. I am looking at a single system which will cover multiple microscopes. I am aware of the need for strict temperature control, no vibration, cooling and pumping redundancy etc. I wondered if anyone else had gone down this path? } } } } ​​​​​ } } Chris } } } } Christopher J. Gilpin Ph.D. } } Director, Life Science Microscopy Facility } } Campus-wide Coordinator for Electron Microscopy } } Purdue University } } Whistler Hall of Agriculture Research, Room S052 } } 170 S. University St } } West Lafayette, IN 47907 } } 765-494-7750 } } gilpin-at-purdue.edu } } lsmf-at-purdue.edu reaches everyone in the facility. } } https://urldefense.proofpoint.com/v2/url?u=http-3A__ag.purdue.edu_arp_Microscopy_Pages_default.aspx&d=DwIBaQ&c=l45AxH-kUV29SRQusp9vYR0n1GycN4_2jInuKy6zbqQ&r=VLPJ8OE-c_C6joGeE1ftlvxMmQPq9N6mpKZONBRt90E&m=csytqarbd0icv1ZERyabHXpB9dtx1JfpHEGZ6SNqRJg&s=MDs2cqGkuLixuFwuHYFtHvZWGEE0SPGhME5zLqwAnZk&e= } } } } } } } } } } ==============================Original Headers============================== } } 7, 32 -- From gilpin-at-purdue.edu Mon Oct 23 09:43:11 2017 } } 7, 32 -- Received: from xppmailspam04.itap.purdue.edu (xppmailspam04.itap.purdue.edu [128.210.5.15]) } } 7, 32 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v9NEhB1a017666 } } 7, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Oct 2017 09:43:11 -0500 } } 7, 32 -- X-IronPort-Anti-Spam-Filtered: true } } 7, 32 -- X-IronPort-AV: E=Sophos;i="5.43,423,1503374400"; } } 7, 32 -- d="scan'208";a="53891616" } } 7, 32 -- Received: from exchange.purdue.edu ([128.210.1.29]) } } 7, 32 -- by xppmailspam04.itap.purdue.edu with ESMTP/TLS/AES256-SHA; 23 Oct 2017 10:38:22 -0400 } } 7, 32 -- Received: from wppexc03.purdue.lcl (172.30.136.176) by wppexc08.purdue.lcl } } 7, 32 -- (172.30.136.181) with Microsoft SMTP Server (TLS) id 15.0.1178.4; Mon, 23 Oct } } 7, 32 -- 2017 10:38:22 -0400 } } 7, 32 -- Received: from wppexc03.purdue.lcl ([fe80::3c86:dcb8:fd99:f174]) by } } 7, 32 -- wppexc03.purdue.lcl ([fe80::3c86:dcb8:fd99:f174%15]) with mapi id } } 7, 32 -- 15.00.1178.000; Mon, 23 Oct 2017 10:38:22 -0400 } } 7, 32 -- From: "Gilpin, Christopher J" {gilpin-at-purdue.edu} } } 7, 32 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} } } 7, 32 -- Subject: novel chilled water circulator systems } } 7, 32 -- Thread-Topic: novel chilled water circulator systems } } 7, 32 -- Thread-Index: AdNMDJK6buGxezYqSHCaJWTqaxkiHw== } } 7, 32 -- Date: Mon, 23 Oct 2017 14:38:21 +0000 } } 7, 32 -- Message-ID: {437c283aab224a0ca1c929b57208d9ee-at-wppexc03.purdue.lcl} } } 7, 32 -- Accept-Language: en-US } } 7, 32 -- Content-Language: en-US } } 7, 32 -- X-MS-Has-Attach: } } 7, 32 -- X-MS-TNEF-Correlator: } } 7, 32 -- x-ms-exchange-transport-fromentityheader: Hosted } } 7, 32 -- x-originating-ip: [10.163.23.200] } } 7, 32 -- Content-Type: text/plain; charset="utf-8" } } 7, 32 -- MIME-Version: 1.0 } } 7, 32 -- Content-Transfer-Encoding: 8bit } } 7, 32 -- X-MIME-Autoconverted: from base64 to 8bit by microscopy.com id v9NEhB1a017666 } } ==============================End of - Headers============================== } } } } -- } =============================================== } } Do not reply to this messages it is from } the Microscopy Listserver NO-REPLY forwarding } system. You should send a new message to } } Microscopy-at-Microscopy.com } } ============================================= } } ==============================Original Headers============================== } 18, 52 -- From microscopy.listserver-at-gmail.com Tue Oct 24 06:17:19 2017 } 18, 52 -- Received: from mail-io0-f180.google.com (mail-io0-f180.google.com [209.85.223.180]) } 18, 52 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v9OBHJVZ027076 } 18, 52 -- for {microscopy-at-microscopy.com} ; Tue, 24 Oct 2017 06:17:19 -0500 } 18, 52 -- Received: by mail-io0-f180.google.com with SMTP id m16so23471122iod.1 } 18, 52 -- for {microscopy-at-microscopy.com} ; Tue, 24 Oct 2017 04:12:34 -0700 (PDT) } 18, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 18, 52 -- d=gmail.com; s=20161025; } 18, 52 -- h=subject:to:references:from:message-id:date:user-agent:mime-version } 18, 52 -- :in-reply-to:content-language:content-transfer-encoding; } 18, 52 -- bh=iADeRoPxVLZHMLOAZjJ1T4d1YD7+1qV2lE88SMYr/7U=; } 18, 52 -- b=HwcxeSDwRisAiKEx5GxVlxHGUNiQRGkx6vRAs6bYft5Nspiyglz5J5R2sPz3m/EMiy } 18, 52 -- R0gdKHeH//xhRkSlPKkUsWg/jk/n30ly+IRhjU+Z6PQmb9Iz7kk68oL9KaWEF/cL8+ny } 18, 52 -- bUHGNwWKPLbZWctiy1XsxzzOAE7llWq8eWOTdVv4MbdKFOBUUhgrDYAOKdCBgii+seHX } 18, 52 -- jOIkixk7NXCRr6JmtDanooxnHrjvxndNS9P2E+TTeUuzY235Klu37/HJcObwKnnvUuuS } 18, 52 -- HCHGphxVIhDwAV6Pqgyz+IN+v7sWk88M9BJzGcsbCv5VCP+Zfl2hNCQMocKbSuPBTuHX } 18, 52 -- LZ3g== } 18, 52 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 18, 52 -- d=1e100.net; s=20161025; } 18, 52 -- h=x-gm-message-state:subject:to:references:from:message-id:date } 18, 52 -- :user-agent:mime-version:in-reply-to:content-language } 18, 52 -- :content-transfer-encoding; } 18, 52 -- bh=iADeRoPxVLZHMLOAZjJ1T4d1YD7+1qV2lE88SMYr/7U=; } 18, 52 -- b=aCv4ywN+4RathLmlEVEe5nc/yv5d5SDwqGVbCmL3Q048xUNn3d2Bnk4NoF4Ad51S/u } 18, 52 -- GYDGaw3wsbhITiqnuiSLmT/6E4++q2G63eNIb0fFeMvj51TN344Q6o9OQXBRed7HiyGV } 18, 52 -- OB+eZVUA8Ibe+qBP046I41wRIQHL+o3RiLWIlFEJ9AcosF1AFmt81XwyJbMWQ1z+0dJ9 } 18, 52 -- Ajohcczg049IGzAfcs4FreYQ187Hml3v25TcHJa3daNXTwN3Ohue4hFoRb19IsyWN3ZM } 18, 52 -- Kh08KEPgZFiF5OJsBXAteXekGa/eOSNDDosGgnTZ4M8N4HyFQ0EE604WZQL+WZ5RHTdI } 18, 52 -- VtDw== } 18, 52 -- X-Gm-Message-State: AMCzsaUKAEZcH0QtZJtZxhJ3ly4970YjNLzGk81gn4Yju2lI7AFuEUyi } 18, 52 -- 9NksZ89USRSRRrHIjTouR+V2fsv9 } 18, 52 -- X-Google-Smtp-Source: ABhQp+SBtCwuiBWpBD9OC46m2qKP5GIVxR9eLpFbQUcFgq74zT/7DLR8sRgFrJWIIhawP6f/W655JA== } 18, 52 -- X-Received: by 10.107.198.12 with SMTP id w12mr21333736iof.151.1508843553471; } 18, 52 -- Tue, 24 Oct 2017 04:12:33 -0700 (PDT) } 18, 52 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:64fc:5200:1154:c57b]) } 18, 52 -- by smtp.googlemail.com with ESMTPSA id s5sm37821its.1.2017.10.24.04.12.32 } 18, 52 -- for {microscopy-at-microscopy.com} } 18, 52 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 18, 52 -- Tue, 24 Oct 2017 04:12:33 -0700 (PDT) } 18, 52 -- Subject: Re: [Microscopy] novel chilled water circulator systems } 18, 52 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 18, 52 -- References: {201710231503.v9NF3fcI005549-at-microscopy.com} } 18, 52 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 18, 52 -- Message-ID: {33c542b8-c9fb-9d26-4cc4-72a1ad58d57e-at-gmail.com} } 18, 52 -- Date: Tue, 24 Oct 2017 06:12:32 -0500 } 18, 52 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) } 18, 52 -- Gecko/20100101 Thunderbird/52.4.0 } 18, 52 -- MIME-Version: 1.0 } 18, 52 -- In-Reply-To: {201710231503.v9NF3fcI005549-at-microscopy.com} } 18, 52 -- Content-Type: text/plain; charset=utf-8; format=flowed } 18, 52 -- Content-Language: en-US } 18, 52 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers==============================
Unless setup includes instant switching to city water in emergency by simply turning a few valves. Assuming cooling by city water is suitable (policy, water quality/temperature, etc.).
Vitaly Feingold SIA 2773 Heath Lane Duluth GA 30096 Ph. 770-232-7785 Fax 770-232-1791 www.sia-cam.com vitaly-at-sia-cam.com
On 10/24/2017 7:19 AM, microscopy.listserver-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Chris } } Don't do this. We tried it at ANL, had a special commerical high capacity unit designed and built to } run all the instruments. } } Individual units on each instrument, all of them cooled by a central cooling system is better. } } } When your SINGLE cooling water system goes down (and it will) then all instruments } are dead in the water. However, if the central chiller which services the small units goes down, } it can still be replaced by a emergency house water line. Since the house water line is simply } taking away the heat from the regulated individual units (all of which have their own controllers) } you can keep all the instruments running. } } This also holds true for routine servicing. If you need to change a filter on your water system, } all instruments must be shut down! } } It was, in my opinion, our only mistake in SAMMLab and we now essentially use that large single } system to cool individual units, which is huge overkill. } } Two other points: } } Contamination of the cooling water from all instruments (new and old) gets mixed in the large } single system. If one instrument has a problem and contaminates the water then all instruments } get contaminated. } } Not all instruments run best at the "same temperature". We have had to tune the water temperature of } some units to minimize drift and that temperature is a few degree's different than the other } microscopes. This won't likely be an issue with SEM's, but it can be for HREM/AEM's. } } Nestor } Your Friendly Neighborhood SysOp } } } } On 10/23/17 10:03 AM CDT, gilpin-at-purdue.edu wrote: } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } } } Hi everyone, } } I have the opportunity to change away from individual chiller units attached to SEMs and TEMs and to work with our physical facilities to design, build and run something new. I am looking at a single system which will cover multiple microscopes. I am aware of the need for strict temperature control, no vibration, cooling and pumping redundancy etc. I wondered if anyone else had gone down this path? } } } } ​​​​​ } } Chris } } } } Christopher J. Gilpin Ph.D. } } Director, Life Science Microscopy Facility } } Campus-wide Coordinator for Electron Microscopy } } Purdue University } } Whistler Hall of Agriculture Research, Room S052 } } 170 S. University St } } West Lafayette, IN 47907 } } 765-494-7750 } } gilpin-at-purdue.edu } } lsmf-at-purdue.edu reaches everyone in the facility. } } http://ag.purdue.edu/arp/Microscopy/Pages/default.aspx } } } } } } } } } } ==============================Original Headers============================== } } 7, 32 -- From gilpin-at-purdue.edu Mon Oct 23 09:43:11 2017 } } 7, 32 -- Received: from xppmailspam04.itap.purdue.edu (xppmailspam04.itap.purdue.edu [128.210.5.15]) } } 7, 32 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v9NEhB1a017666 } } 7, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Oct 2017 09:43:11 -0500 } } 7, 32 -- X-IronPort-Anti-Spam-Filtered: true } } 7, 32 -- X-IronPort-AV: E=Sophos;i="5.43,423,1503374400"; } } 7, 32 -- d="scan'208";a="53891616" } } 7, 32 -- Received: from exchange.purdue.edu ([128.210.1.29]) } } 7, 32 -- by xppmailspam04.itap.purdue.edu with ESMTP/TLS/AES256-SHA; 23 Oct 2017 10:38:22 -0400 } } 7, 32 -- Received: from wppexc03.purdue.lcl (172.30.136.176) by wppexc08.purdue.lcl } } 7, 32 -- (172.30.136.181) with Microsoft SMTP Server (TLS) id 15.0.1178.4; Mon, 23 Oct } } 7, 32 -- 2017 10:38:22 -0400 } } 7, 32 -- Received: from wppexc03.purdue.lcl ([fe80::3c86:dcb8:fd99:f174]) by } } 7, 32 -- wppexc03.purdue.lcl ([fe80::3c86:dcb8:fd99:f174%15]) with mapi id } } 7, 32 -- 15.00.1178.000; Mon, 23 Oct 2017 10:38:22 -0400 } } 7, 32 -- From: "Gilpin, Christopher J" {gilpin-at-purdue.edu} } } 7, 32 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} } } 7, 32 -- Subject: novel chilled water circulator systems } } 7, 32 -- Thread-Topic: novel chilled water circulator systems } } 7, 32 -- Thread-Index: AdNMDJK6buGxezYqSHCaJWTqaxkiHw== } } 7, 32 -- Date: Mon, 23 Oct 2017 14:38:21 +0000 } } 7, 32 -- Message-ID: {437c283aab224a0ca1c929b57208d9ee-at-wppexc03.purdue.lcl} } } 7, 32 -- Accept-Language: en-US } } 7, 32 -- Content-Language: en-US } } 7, 32 -- X-MS-Has-Attach: } } 7, 32 -- X-MS-TNEF-Correlator: } } 7, 32 -- x-ms-exchange-transport-fromentityheader: Hosted } } 7, 32 -- x-originating-ip: [10.163.23.200] } } 7, 32 -- Content-Type: text/plain; charset="utf-8" } } 7, 32 -- MIME-Version: 1.0 } } 7, 32 -- Content-Transfer-Encoding: 8bit } } 7, 32 -- X-MIME-Autoconverted: from base64 to 8bit by microscopy.com id v9NEhB1a017666 } } ==============================End of - Headers============================== } }
Name:Lita Drain School:Howard Hughes Medical Institute
US Email:duraine-at-bcm.edu {mailto:duraine-at-bcm.eduSubject}
Subject {mailto:duraine-at-bcm.eduSubject} :[Microscopy] Re: novel chilled water circulator systems Your Question:I absolutely agree with Nestor. We have a JEOL 1010 TEM and a JEOL 1400 TEM, both on the same larger chiller. To me it is a pain. When the chiller goes out, both TEMs have to be turned off. We just had a power failure in the one TEM room, but since the one chiller is connected to that room then the chiller went out too. Because of that I had to run to turn off the other TEM in the next separate room. Both instruments were down until I could get power back on. Because of the power failure, the compressor tripped in the chiller and both TEMs were down another day until JEOL could come out and fix it for us. If I had separate chillers, then I would still be in operation with the one TEM. There are many other stories but I won't digress...... Don't do it.....buy separate chillers for each instrument, you will be happier for it.
From kimgarr459eaus-at-gmail.com Tue Oct 24 17:19:19 2017 Return-Path: {kimgarr459eaus-at-gmail.com} Received: from gmail.com ([45.115.242.241]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v9OMJIuf005796 for {microscopylistserverarchive6-at-microscopy.com} ; Tue, 24 Oct 2017 17:19:19 -0500 Received: from external.newsubdomain.com ([Tue, 24 Oct 2017 14:57:34 -0700]) by mxs.perenter.com with SMTP; Tue, 24 Oct 2017 14:57:34 -0700 Message-ID: {C88C3900.AF87B873-at-gmail.com}
X-from: John Donovan {donovan-at-uoregon.edu}
I agree with Chris. We have the domestic water tied into the process water system so they can be switched over for maintenance, while as he said, the individual chillers still run each instrument.
Best to have an over temp and flow alarm on the process water system with an alert to your facilities people so they know when it goes down.
john
On 10/24/2017 4:24 AM, microscopy.listserver-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Chris } } Don't do this. We tried it at ANL, had a special commerical high capacity unit designed and built to } run all the instruments. } } Individual units on each instrument, all of them cooled by a central cooling system is better. } } } When your SINGLE cooling water system goes down (and it will) then all instruments } are dead in the water. However, if the central chiller which services the small units goes down, } it can still be replaced by a emergency house water line. Since the house water line is simply } taking away the heat from the regulated individual units (all of which have their own controllers) } you can keep all the instruments running. } } This also holds true for routine servicing. If you need to change a filter on your water system, } all instruments must be shut down! } } It was, in my opinion, our only mistake in SAMMLab and we now essentially use that large single } system to cool individual units, which is huge overkill. } } Two other points: } } Contamination of the cooling water from all instruments (new and old) gets mixed in the large } single system. If one instrument has a problem and contaminates the water then all instruments } get contaminated. } } Not all instruments run best at the "same temperature". We have had to tune the water temperature of } some units to minimize drift and that temperature is a few degree's different than the other } microscopes. This won't likely be an issue with SEM's, but it can be for HREM/AEM's. } } Nestor } Your Friendly Neighborhood SysOp } } } } On 10/23/17 10:03 AM CDT, gilpin-at-purdue.edu wrote: } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } } } Hi everyone, } } I have the opportunity to change away from individual chiller units attached to SEMs and TEMs and to work with our physical facilities to design, build and run something new. I am looking at a single system which will cover multiple microscopes. I am aware of the need for strict temperature control, no vibration, cooling and pumping redundancy etc. I wondered if anyone else had gone down this path? } } } } ​​​​​ } } Chris } } } } Christopher J. Gilpin Ph.D. } } Director, Life Science Microscopy Facility } } Campus-wide Coordinator for Electron Microscopy } } Purdue University } } Whistler Hall of Agriculture Research, Room S052 } } 170 S. University St } } West Lafayette, IN 47907 } } 765-494-7750 } } gilpin-at-purdue.edu } } lsmf-at-purdue.edu reaches everyone in the facility. } } http://ag.purdue.edu/arp/Microscopy/Pages/default.aspx } } } } } } } } } } ==============================Original Headers============================== } } 7, 32 -- From gilpin-at-purdue.edu Mon Oct 23 09:43:11 2017 } } 7, 32 -- Received: from xppmailspam04.itap.purdue.edu (xppmailspam04.itap.purdue.edu [128.210.5.15]) } } 7, 32 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v9NEhB1a017666 } } 7, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Oct 2017 09:43:11 -0500 } } 7, 32 -- X-IronPort-Anti-Spam-Filtered: true } } 7, 32 -- X-IronPort-AV: E=Sophos;i="5.43,423,1503374400"; } } 7, 32 -- d="scan'208";a="53891616" } } 7, 32 -- Received: from exchange.purdue.edu ([128.210.1.29]) } } 7, 32 -- by xppmailspam04.itap.purdue.edu with ESMTP/TLS/AES256-SHA; 23 Oct 2017 10:38:22 -0400 } } 7, 32 -- Received: from wppexc03.purdue.lcl (172.30.136.176) by wppexc08.purdue.lcl } } 7, 32 -- (172.30.136.181) with Microsoft SMTP Server (TLS) id 15.0.1178.4; Mon, 23 Oct } } 7, 32 -- 2017 10:38:22 -0400 } } 7, 32 -- Received: from wppexc03.purdue.lcl ([fe80::3c86:dcb8:fd99:f174]) by } } 7, 32 -- wppexc03.purdue.lcl ([fe80::3c86:dcb8:fd99:f174%15]) with mapi id } } 7, 32 -- 15.00.1178.000; Mon, 23 Oct 2017 10:38:22 -0400 } } 7, 32 -- From: "Gilpin, Christopher J" {gilpin-at-purdue.edu} } } 7, 32 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} } } 7, 32 -- Subject: novel chilled water circulator systems } } 7, 32 -- Thread-Topic: novel chilled water circulator systems } } 7, 32 -- Thread-Index: AdNMDJK6buGxezYqSHCaJWTqaxkiHw== } } 7, 32 -- Date: Mon, 23 Oct 2017 14:38:21 +0000 } } 7, 32 -- Message-ID: {437c283aab224a0ca1c929b57208d9ee-at-wppexc03.purdue.lcl} } } 7, 32 -- Accept-Language: en-US } } 7, 32 -- Content-Language: en-US } } 7, 32 -- X-MS-Has-Attach: } } 7, 32 -- X-MS-TNEF-Correlator: } } 7, 32 -- x-ms-exchange-transport-fromentityheader: Hosted } } 7, 32 -- x-originating-ip: [10.163.23.200] } } 7, 32 -- Content-Type: text/plain; charset="utf-8" } } 7, 32 -- MIME-Version: 1.0 } } 7, 32 -- Content-Transfer-Encoding: 8bit } } 7, 32 -- X-MIME-Autoconverted: from base64 to 8bit by microscopy.com id v9NEhB1a017666 } } ==============================End of - Headers============================== } }
For water filtration, it helps to install the plumbing and canisters for a redundant pair of parallel filters with valves on both sides of each filter unit. That way a single filter can be changed without shutting down the water supply.
If you're really stingy, you can even use this to squeeze a little extra life of a filter which no longer provides enough flow on its own. Just use one filter alone at first, and when the flow/pressure begins to drop below acceptable levels, open the valves on the second filter just a little.
- Steve Kuehn
microscopy.listserver-at-gmail.com wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Chris } } Don't do this. We tried it at ANL, had a special commerical high capacity unit designed and built to } run all the instruments. } } Individual units on each instrument, all of them cooled by a central cooling system is better. } } } When your SINGLE cooling water system goes down (and it will) then all instruments } are dead in the water. However, if the central chiller which services the small units goes down, } it can still be replaced by a emergency house water line. Since the house water line is simply } taking away the heat from the regulated individual units (all of which have their own controllers) } you can keep all the instruments running. } } This also holds true for routine servicing. If you need to change a filter on your water system, } all instruments must be shut down! } } It was, in my opinion, our only mistake in SAMMLab and we now essentially use that large single } system to cool individual units, which is huge overkill. } } Two other points: } } Contamination of the cooling water from all instruments (new and old) gets mixed in the large } single system. If one instrument has a problem and contaminates the water then all instruments } get contaminated. } } Not all instruments run best at the "same temperature". We have had to tune the water temperature of } some units to minimize drift and that temperature is a few degree's different than the other } microscopes. This won't likely be an issue with SEM's, but it can be for HREM/AEM's. } } Nestor } Your Friendly Neighborhood SysOp } } } } On 10/23/17 10:03 AM CDT, gilpin-at-purdue.edu wrote: } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } } } Hi everyone, } } I have the opportunity to change away from individual chiller units attached to SEMs and TEMs and to work with our physical facilities to design, build and run something new. I am looking at a single system which will cover multiple microscopes. I am aware of the need for strict temperature control, no vibration, cooling and pumping redundancy etc. I wondered if anyone else had gone down this path? } } } } ââ¬â¹Ã¢â¬â¹Ã¢â¬â¹Ã¢â¬â¹Ã¢â¬â¹ } } Chris } } } } Christopher J. Gilpin Ph.D. } } Director, Life Science Microscopy Facility } } Campus-wide Coordinator for Electron Microscopy } } Purdue University } } Whistler Hall of Agriculture Research, Room S052 } } 170 S. University St } } West Lafayette, IN 47907 } } 765-494-7750 } } gilpin-at-purdue.edu } } lsmf-at-purdue.edu reaches everyone in the facility. } } http://ag.purdue.edu/arp/Microscopy/Pages/default.aspx } } } } } } } } } } ==============================Original Headers============================== } } 7, 32 -- From gilpin-at-purdue.edu Mon Oct 23 09:43:11 2017 } } 7, 32 -- Received: from xppmailspam04.itap.purdue.edu (xppmailspam04.itap.purdue.edu [128.210.5.15]) } } 7, 32 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v9NEhB1a017666 } } 7, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Oct 2017 09:43:11 -0500 } } 7, 32 -- X-IronPort-Anti-Spam-Filtered: true } } 7, 32 -- X-IronPort-AV: E=Sophos;i="5.43,423,1503374400"; } } 7, 32 -- d="scan'208";a="53891616" } } 7, 32 -- Received: from exchange.purdue.edu ([128.210.1.29]) } } 7, 32 -- by xppmailspam04.itap.purdue.edu with ESMTP/TLS/AES256-SHA; 23 Oct 2017 10:38:22 -0400 } } 7, 32 -- Received: from wppexc03.purdue.lcl (172.30.136.176) by wppexc08.purdue.lcl } } 7, 32 -- (172.30.136.181) with Microsoft SMTP Server (TLS) id 15.0.1178.4; Mon, 23 Oct } } 7, 32 -- 2017 10:38:22 -0400 } } 7, 32 -- Received: from wppexc03.purdue.lcl ([fe80::3c86:dcb8:fd99:f174]) by } } 7, 32 -- wppexc03.purdue.lcl ([fe80::3c86:dcb8:fd99:f174%15]) with mapi id } } 7, 32 -- 15.00.1178.000; Mon, 23 Oct 2017 10:38:22 -0400 } } 7, 32 -- From: "Gilpin, Christopher J" {gilpin-at-purdue.edu} } } 7, 32 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} } } 7, 32 -- Subject: novel chilled water circulator systems } } 7, 32 -- Thread-Topic: novel chilled water circulator systems } } 7, 32 -- Thread-Index: AdNMDJK6buGxezYqSHCaJWTqaxkiHw== } } 7, 32 -- Date: Mon, 23 Oct 2017 14:38:21 +0000 } } 7, 32 -- Message-ID: {437c283aab224a0ca1c929b57208d9ee-at-wppexc03.purdue.lcl} } } 7, 32 -- Accept-Language: en-US } } 7, 32 -- Content-Language: en-US } } 7, 32 -- X-MS-Has-Attach: } } 7, 32 -- X-MS-TNEF-Correlator: } } 7, 32 -- x-ms-exchange-transport-fromentityheader: Hosted } } 7, 32 -- x-originating-ip: [10.163.23.200] } } 7, 32 -- Content-Type: text/plain; charset="utf-8" } } 7, 32 -- MIME-Version: 1.0 } } 7, 32 -- Content-Transfer-Encoding: 8bit } } 7, 32 -- X-MIME-Autoconverted: from base64 to 8bit by microscopy.com id v9NEhB1a017666 } } ==============================End of - Headers============================== } }
--
---------------- Dr. Stephen C. Kuehn Associate Professor Manager, Electron Microprobe Facility & Tephra Lab Science building, Room 106
Concord University 1000 Vermillion St PO Box 1000, Campus Box F20 Athens, WV 24712-1000
I totally agree. We have individual chillers for each instrument and all chillers are connected to the building chilled processed water. So it is much easier to service individual chillers without shutting down all scopes.
On Tue, Oct 24, 2017 at 7:27 AM, {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote:
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver On-Line {http://www.microscopy.com/MicroscopyListserver On-Line} Help http://www.microscopy.com/MicroscopyListserver/FAQ.html {http://www.microscopy.com/MicroscopyListserver/FAQ.html} ----------------------------------------------------------------------------
Chris
Don't do this. We tried it at ANL, had a special commerical high capacity unit designed and built to run all the instruments.
Individual units on each instrument, all of them cooled by a central cooling system is better.
When your SINGLE cooling water system goes down (and it will) then all instruments are dead in the water. However, if the central chiller which services the small units goes down, it can still be replaced by a emergency house water line. Since the house water line is simply taking away the heat from the regulated individual units (all of which have their own controllers) you can keep all the instruments running.
This also holds true for routine servicing. If you need to change a filter on your water system, all instruments must be shut down!
It was, in my opinion, our only mistake in SAMMLab and we now essentially use that large single system to cool individual units, which is huge overkill.
Two other points:
Contamination of the cooling water from all instruments (new and old) gets mixed in the large single system. If one instrument has a problem and contaminates the water then all instruments get contaminated.
Not all instruments run best at the "same temperature". We have had to tune the water temperature of some units to minimize drift and that temperature is a few degree's different than the other microscopes. This won't likely be an issue with SEM's, but it can be for HREM/AEM's.
Nestor Your Friendly Neighborhood SysOp
On 10/23/17 10:03 AM CDT, gilpin-at-purdue.edu {mailto:gilpin-at-purdue.edu} wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver {http://www.microscopy.com/MicroscopyListserver} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html {http://www.microscopy.com/MicroscopyListserver/FAQ.html} } ---------------------------------------------------------------------------- } } } Hi everyone, } I have the opportunity to change away from individual chiller units attached to SEMs and TEMs and to work with our physical facilities to design, build and run something new. I am looking at a single system which will cover multiple microscopes. I am aware of the need for strict temperature control, no vibration, cooling and pumping redundancy etc. I wondered if anyone else had gone down this path? } } ​​​​​ } Chris } } Christopher J. Gilpin Ph.D. } Director, Life Science Microscopy Facility } Campus-wide Coordinator for Electron Microscopy } Purdue University } Whistler Hall of Agriculture Research, Room S052 } 170 S. University St } West Lafayette, IN 47907 } 765-494-7750 {tel:765-494-7750} } gilpin-at-purdue.edu {mailto:gilpin-at-purdue.edu} } lsmf-at-purdue.edu {mailto:lsmf-at-purdue.edu} reaches everyone in the facility. } http://ag.purdue.edu/arp/Microscopy/Pages/default.aspx {http://ag.purdue.edu/arp/Microscopy/Pages/default.aspx} } } } } } ==============================Original Headers============================== } 7, 32 -- From gilpin-at-purdue.edu {mailto:gilpin-at-purdue.edu} Mon Oct 23 09:43:11 2017 } 7, 32 -- Received: from xppmailspam04.itap.purdue.edu {http://xppmailspam04.itap.purdue.edu} (xppmailspam04.itap.purdue.edu {http://xppmailspam04.itap.purdue.edu} [128.210.5.15]) } 7, 32 -- by microscopy.com {http://microscopy.com} (8.12.11.20060308/8.12.8) with ESMTP id v9NEhB1a017666 } 7, 32 -- for {Microscopy-at-microscopy.com {mailto:Microscopy-at-microscopy.com} } ; Mon, 23 Oct 2017 09:43:11 -0500 } 7, 32 -- X-IronPort-Anti-Spam-Filtered: true } 7, 32 -- X-IronPort-AV: E=Sophos;i="5.43,423,1503374400"; } 7, 32 -- d="scan'208";a="53891616" } 7, 32 -- Received: from exchange.purdue.edu {http://exchange.purdue.edu} ([128.210.1.29]) } 7, 32 -- by xppmailspam04.itap.purdue.edu {http://xppmailspam04.itap.purdue.edu} with ESMTP/TLS/AES256-SHA; 23 Oct 2017 10:38:22 -0400 } 7, 32 -- Received: from wppexc03.purdue.lcl (172.30.136.176) by wppexc08.purdue.lcl } 7, 32 -- (172.30.136.181) with Microsoft SMTP Server (TLS) id 15.0.1178.4; Mon, 23 Oct } 7, 32 -- 2017 10:38:22 -0400 } 7, 32 -- Received: from wppexc03.purdue.lcl ([fe80::3c86:dcb8:fd99:f174]) by } 7, 32 -- wppexc03.purdue.lcl ([fe80::3c86:dcb8:fd99:f174%15]) with mapi id } 7, 32 -- 15.00.1178.000; Mon, 23 Oct 2017 10:38:22 -0400 } 7, 32 -- From: "Gilpin, Christopher J" {gilpin-at-purdue.edu {mailto:gilpin-at-purdue.edu} } } 7, 32 -- To: "Microscopy-at-microscopy.com {mailto:Microscopy-at-microscopy.com} " {Microscopy-at-microscopy.com {mailto:Microscopy-at-microscopy.com} } } 7, 32 -- Subject: novel chilled water circulator systems } 7, 32 -- Thread-Topic: novel chilled water circulator systems } 7, 32 -- Thread-Index: AdNMDJK6buGxezYqSHCaJWTqaxkiHw== } 7, 32 -- Date: Mon, 23 Oct 2017 14:38:21 +0000 } 7, 32 -- Message-ID: {437c283aab224a0ca1c929b57208d9ee-at-wppexc03.purdue.lcl} } 7, 32 -- Accept-Language: en-US } 7, 32 -- Content-Language: en-US } 7, 32 -- X-MS-Has-Attach: } 7, 32 -- X-MS-TNEF-Correlator: } 7, 32 -- x-ms-exchange-transport-fromentityheader: Hosted } 7, 32 -- x-originating-ip: [10.163.23.200] } 7, 32 -- Content-Type: text/plain; charset="utf-8" } 7, 32 -- MIME-Version: 1.0 } 7, 32 -- Content-Transfer-Encoding: 8bit } 7, 32 -- X-MIME-Autoconverted: from base64 to 8bit by microscopy.com {http://microscopy.com} id v9NEhB1a017666 } ==============================End of - Headers============================== }
On Oct 24, 2017, at 7:29 AM, microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Chris } } Don't do this. We tried it at ANL, had a special commerical high capacity unit designed and built to } run all the instruments. } } Individual units on each instrument, all of them cooled by a central cooling system is better. } } } When your SINGLE cooling water system goes down (and it will) then all instruments } are dead in the water. However, if the central chiller which services the small units goes down, } it can still be replaced by a emergency house water line. Since the house water line is simply } taking away the heat from the regulated individual units (all of which have their own controllers) } you can keep all the instruments running. } } This also holds true for routine servicing. If you need to change a filter on your water system, } all instruments must be shut down! } } It was, in my opinion, our only mistake in SAMMLab and we now essentially use that large single } system to cool individual units, which is huge overkill. } } Two other points: } } Contamination of the cooling water from all instruments (new and old) gets mixed in the large } single system. If one instrument has a problem and contaminates the water then all instruments } get contaminated. } } Not all instruments run best at the "same temperature". We have had to tune the water temperature of } some units to minimize drift and that temperature is a few degree's different than the other } microscopes. This won't likely be an issue with SEM's, but it can be for HREM/AEM's. } } Nestor } Your Friendly Neighborhood SysOp } } } } On 10/23/17 10:03 AM CDT, gilpin-at-purdue.edu {mailto:gilpin-at-purdue.edu} wrote: } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } } } Hi everyone, } } I have the opportunity to change away from individual chiller units attached to SEMs and TEMs and } } to work with our physical facilities to design, build and run something new. I am looking at a } } single system which will cover multiple microscopes. I am aware of the need for strict } } temperature control, no vibration, cooling and pumping redundancy etc. I wondered if anyone else } } had gone down this path? } } } } ​​​​​ } } Chris } } } } Christopher J. Gilpin Ph.D. } } Director, Life Science Microscopy Facility } } Campus-wide Coordinator for Electron Microscopy } } Purdue University } } Whistler Hall of Agriculture Research, Room S052 } } 170 S. University St } } West Lafayette, IN 47907 } } 765-494-7750 } } gilpin-at-purdue.edu {mailto:gilpin-at-purdue.edu} } } lsmf-at-purdue.edu {mailto:lsmf-at-purdue.edu} reaches everyone in the facility. } } http://ag.purdue.edu/arp/Microscopy/Pages/default.aspx } } } } } } } } } } ==============================Original Headers============================== } } 7, 32 -- From gilpin-at-purdue.edu {mailto:gilpin-at-purdue.edu} Mon Oct 23 09:43:11 2017 } } 7, 32 -- Received: from xppmailspam04.itap.purdue.edu {http://xppmailspam04.itap.purdue.edu} } } (xppmailspam04.itap.purdue.edu {http://xppmailspam04.itap.purdue.edu} [128.210.5.15]) } } 7, 32 -- by microscopy.com {http://microscopy.com} (8.12.11.20060308/8.12.8) with ESMTP id } } v9NEhB1a017666 } } 7, 32 -- for {Microscopy-at-microscopy.com {mailto:Microscopy-at-microscopy.com} } ; Mon, 23 Oct 2017 } } 09:43:11 -0500 } } 7, 32 -- X-IronPort-Anti-Spam-Filtered: true } } 7, 32 -- X-IronPort-AV: E=Sophos;i="5.43,423,1503374400"; } } 7, 32 -- d="scan'208";a="53891616" } } 7, 32 -- Received: from exchange.purdue.edu {http://exchange.purdue.edu} ([128.210.1.29]) } } 7, 32 -- by xppmailspam04.itap.purdue.edu {http://xppmailspam04.itap.purdue.edu} with } } ESMTP/TLS/AES256-SHA; 23 Oct 2017 10:38:22 -0400 } } 7, 32 -- Received: from wppexc03.purdue.lcl (172.30.136.176) by wppexc08.purdue.lcl } } 7, 32 -- (172.30.136.181) with Microsoft SMTP Server (TLS) id 15.0.1178.4; Mon, 23 Oct } } 7, 32 -- 2017 10:38:22 -0400 } } 7, 32 -- Received: from wppexc03.purdue.lcl ([fe80::3c86:dcb8:fd99:f174]) by } } 7, 32 -- wppexc03.purdue.lcl ([fe80::3c86:dcb8:fd99:f174%15]) with mapi id } } 7, 32 -- 15.00.1178.000; Mon, 23 Oct 2017 10:38:22 -0400 } } 7, 32 -- From: "Gilpin, Christopher J" {gilpin-at-purdue.edu {mailto:gilpin-at-purdue.edu} } } } 7, 32 -- To: "Microscopy-at-microscopy.com {mailto:Microscopy-at-microscopy.com} " } } {Microscopy-at-microscopy.com {mailto:Microscopy-at-microscopy.com} } } } 7, 32 -- Subject: novel chilled water circulator systems } } 7, 32 -- Thread-Topic: novel chilled water circulator systems } } 7, 32 -- Thread-Index: AdNMDJK6buGxezYqSHCaJWTqaxkiHw== } } 7, 32 -- Date: Mon, 23 Oct 2017 14:38:21 +0000 } } 7, 32 -- Message-ID: {437c283aab224a0ca1c929b57208d9ee-at-wppexc03.purdue.lcl } } {mailto:437c283aab224a0ca1c929b57208d9ee-at-wppexc03.purdue.lcl} } } } 7, 32 -- Accept-Language: en-US } } 7, 32 -- Content-Language: en-US } } 7, 32 -- X-MS-Has-Attach: } } 7, 32 -- X-MS-TNEF-Correlator: } } 7, 32 -- x-ms-exchange-transport-fromentityheader: Hosted } } 7, 32 -- x-originating-ip: [10.163.23.200] } } 7, 32 -- Content-Type: text/plain; charset="utf-8" } } 7, 32 -- MIME-Version: 1.0 } } 7, 32 -- Content-Transfer-Encoding: 8bit } } 7, 32 -- X-MIME-Autoconverted: from base64 to 8bit by microscopy.com {http://microscopy.com} id } } v9NEhB1a017666 } } ==============================End of - Headers============================== } } } } -- } =============================================== } } Do not reply to this messages it is from } the Microscopy Listserver NO-REPLY forwarding } system. You should send a new message to } } Microscopy-at-Microscopy.com {mailto:Microscopy-at-Microscopy.com} } } ============================================= } } ==============================Original Headers============================== } 18, 52 -- From microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} Tue Oct 24 } 06:17:19 2017 } 18, 52 -- Received: from mail-io0-f180.google.com {http://mail-io0-f180.google.com} } (mail-io0-f180.google.com {http://mail-io0-f180.google.com} [209.85.223.180]) } 18, 52 -- by microscopy.com {http://microscopy.com} (8.12.11.20060308/8.12.8) with ESMTP id } v9OBHJVZ027076 } 18, 52 -- for {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } ; Tue, 24 Oct 2017 } 06:17:19 -0500 } 18, 52 -- Received: by mail-io0-f180.google.com {http://mail-io0-f180.google.com} with SMTP id } m16so23471122iod.1 } 18, 52 -- for {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } ; Tue, 24 Oct } 2017 04:12:34 -0700 (PDT) } 18, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 18, 52 -- d=gmail.com {http://gmail.com} ; s=20161025; } 18, 52 -- h=subject:to:references:from:message-id:date:user-agent:mime-version } 18, 52 -- :in-reply-to:content-language:content-transfer-encoding; } 18, 52 -- bh=iADeRoPxVLZHMLOAZjJ1T4d1YD7+1qV2lE88SMYr/7U=; } 18, 52 -- b=HwcxeSDwRisAiKEx5GxVlxHGUNiQRGkx6vRAs6bYft5Nspiyglz5J5R2sPz3m/EMiy } 18, 52 -- R0gdKHeH//xhRkSlPKkUsWg/jk/n30ly+IRhjU+Z6PQmb9Iz7kk68oL9KaWEF/cL8+ny } 18, 52 -- bUHGNwWKPLbZWctiy1XsxzzOAE7llWq8eWOTdVv4MbdKFOBUUhgrDYAOKdCBgii+seHX } 18, 52 -- jOIkixk7NXCRr6JmtDanooxnHrjvxndNS9P2E+TTeUuzY235Klu37/HJcObwKnnvUuuS } 18, 52 -- HCHGphxVIhDwAV6Pqgyz+IN+v7sWk88M9BJzGcsbCv5VCP+Zfl2hNCQMocKbSuPBTuHX } 18, 52 -- LZ3g== } 18, 52 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 18, 52 -- d=1e100.net {http://1e100.net} ; s=20161025; } 18, 52 -- h=x-gm-message-state:subject:to:references:from:message-id:date } 18, 52 -- :user-agent:mime-version:in-reply-to:content-language } 18, 52 -- :content-transfer-encoding; } 18, 52 -- bh=iADeRoPxVLZHMLOAZjJ1T4d1YD7+1qV2lE88SMYr/7U=; } 18, 52 -- b=aCv4ywN+4RathLmlEVEe5nc/yv5d5SDwqGVbCmL3Q048xUNn3d2Bnk4NoF4Ad51S/u } 18, 52 -- GYDGaw3wsbhITiqnuiSLmT/6E4++q2G63eNIb0fFeMvj51TN344Q6o9OQXBRed7HiyGV } 18, 52 -- OB+eZVUA8Ibe+qBP046I41wRIQHL+o3RiLWIlFEJ9AcosF1AFmt81XwyJbMWQ1z+0dJ9 } 18, 52 -- Ajohcczg049IGzAfcs4FreYQ187Hml3v25TcHJa3daNXTwN3Ohue4hFoRb19IsyWN3ZM } 18, 52 -- Kh08KEPgZFiF5OJsBXAteXekGa/eOSNDDosGgnTZ4M8N4HyFQ0EE604WZQL+WZ5RHTdI } 18, 52 -- VtDw== } 18, 52 -- X-Gm-Message-State: AMCzsaUKAEZcH0QtZJtZxhJ3ly4970YjNLzGk81gn4Yju2lI7AFuEUyi } 18, 52 -- 9NksZ89USRSRRrHIjTouR+V2fsv9 } 18, 52 -- X-Google-Smtp-Source: } ABhQp+SBtCwuiBWpBD9OC46m2qKP5GIVxR9eLpFbQUcFgq74zT/7DLR8sRgFrJWIIhawP6f/W655JA== } 18, 52 -- X-Received: by 10.107.198.12 with SMTP id w12mr21333736iof.151.1508843553471; } 18, 52 -- Tue, 24 Oct 2017 04:12:33 -0700 (PDT) } 18, 52 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net } {http://96-65-115-77-static.hfc.comcastbusiness.net} ([2603:300a:f04:7100:64fc:5200:1154:c57b]) } 18, 52 -- by smtp.googlemail.com {http://smtp.googlemail.com} with ESMTPSA id } s5sm37821its.1.2017.10.24.04.12.32 } 18, 52 -- for {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } } 18, 52 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 18, 52 -- Tue, 24 Oct 2017 04:12:33 -0700 (PDT) } 18, 52 -- Subject: Re: [Microscopy] novel chilled water circulator systems } 18, 52 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com } {mailto:microscopy-at-microscopy.com} } } 18, 52 -- References: {201710231503.v9NF3fcI005549-at-microscopy.com } {mailto:201710231503.v9NF3fcI005549-at-microscopy.com} } } 18, 52 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com } {mailto:microscopy.listserver-at-gmail.com} } } 18, 52 -- Message-ID: {33c542b8-c9fb-9d26-4cc4-72a1ad58d57e-at-gmail.com } {mailto:33c542b8-c9fb-9d26-4cc4-72a1ad58d57e-at-gmail.com} } } 18, 52 -- Date: Tue, 24 Oct 2017 06:12:32 -0500 } 18, 52 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) } 18, 52 -- Gecko/20100101 Thunderbird/52.4.0 } 18, 52 -- MIME-Version: 1.0 } 18, 52 -- In-Reply-To: {201710231503.v9NF3fcI005549-at-microscopy.com } {mailto:201710231503.v9NF3fcI005549-at-microscopy.com} } } 18, 52 -- Content-Type: text/plain; charset=utf-8; format=flowed } 18, 52 -- Content-Language: en-US } 18, 52 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers==============================
Princeton University and the Philadelphia Society for Microscopy will hold a Symposium on Thursday November 2^nd . We will have 3 talks in the morning and early afternoon, followed by tours of Princeton’s microscopy facilities.
You can get more information and register at the following:
We run a main chiller to cool individual chillers. When main chiller goes down, there is an automatic switch to city water to provide cooling.
John
-----Original Message----- X-from: gilpin-at-purdue.edu [mailto:gilpin-at-purdue.edu] Sent: Monday, October 23, 2017 10:45 AM To: John W. Catino {john.catino-at-mineralstech.com}
Hi everyone, I have the opportunity to change away from individual chiller units attached to SEMs and TEMs and to work with our physical facilities to design, build and run something new. I am looking at a single system which will cover multiple microscopes. I am aware of the need for strict temperature control, no vibration, cooling and pumping redundancy etc. I wondered if anyone else had gone down this path?
Chris
Christopher J. Gilpin Ph.D. Director, Life Science Microscopy Facility Campus-wide Coordinator for Electron Microscopy Purdue University Whistler Hall of Agriculture Research, Room S052 170 S. University St West Lafayette, IN 47907 765-494-7750 gilpin-at-purdue.edu lsmf-at-purdue.edu reaches everyone in the facility. http://ag.purdue.edu/arp/Microscopy/Pages/default.aspx
==============================Original Headers============================== 7, 32 -- From gilpin-at-purdue.edu Mon Oct 23 09:43:11 2017 7, 32 -- Received: from xppmailspam04.itap.purdue.edu (xppmailspam04.itap.purdue.edu [128.210.5.15]) 7, 32 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v9NEhB1a017666 7, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Oct 2017 09:43:11 -0500 7, 32 -- X-IronPort-Anti-Spam-Filtered: true 7, 32 -- X-IronPort-AV: E=Sophos;i="5.43,423,1503374400"; 7, 32 -- d="scan'208";a="53891616" 7, 32 -- Received: from exchange.purdue.edu ([128.210.1.29]) 7, 32 -- by xppmailspam04.itap.purdue.edu with ESMTP/TLS/AES256-SHA; 23 Oct 2017 10:38:22 -0400 7, 32 -- Received: from wppexc03.purdue.lcl (172.30.136.176) by wppexc08.purdue.lcl 7, 32 -- (172.30.136.181) with Microsoft SMTP Server (TLS) id 15.0.1178.4; Mon, 23 Oct 7, 32 -- 2017 10:38:22 -0400 7, 32 -- Received: from wppexc03.purdue.lcl ([fe80::3c86:dcb8:fd99:f174]) by 7, 32 -- wppexc03.purdue.lcl ([fe80::3c86:dcb8:fd99:f174%15]) with mapi id 7, 32 -- 15.00.1178.000; Mon, 23 Oct 2017 10:38:22 -0400 7, 32 -- From: "Gilpin, Christopher J" {gilpin-at-purdue.edu} 7, 32 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 7, 32 -- Subject: novel chilled water circulator systems 7, 32 -- Thread-Topic: novel chilled water circulator systems 7, 32 -- Thread-Index: AdNMDJK6buGxezYqSHCaJWTqaxkiHw== 7, 32 -- Date: Mon, 23 Oct 2017 14:38:21 +0000 7, 32 -- Message-ID: {437c283aab224a0ca1c929b57208d9ee-at-wppexc03.purdue.lcl} 7, 32 -- Accept-Language: en-US 7, 32 -- Content-Language: en-US 7, 32 -- X-MS-Has-Attach: 7, 32 -- X-MS-TNEF-Correlator: 7, 32 -- x-ms-exchange-transport-fromentityheader: Hosted 7, 32 -- x-originating-ip: [10.163.23.200] 7, 32 -- Content-Type: text/plain; charset="utf-8" 7, 32 -- MIME-Version: 1.0 7, 32 -- Content-Transfer-Encoding: 8bit 7, 32 -- X-MIME-Autoconverted: from base64 to 8bit by microscopy.com id v9NEhB1a017666 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both frederick.meisenkothen-at-nist.gov as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: frederick.meisenkothen-at-nist.gov Name: Fred Meisenkothen
Organization: NIST
Title-Subject: [Filtered] Atom Probe Tomography and Microscopy 2018 (APT&M 2018)
Message: The Call for Papers is out for the APT&M 2018 Meeting to be held at NIST, Gaithersburg MD on June 10-15, 2018. {https://www.nist.gov/news-events/events/2018/06/atom-probe-tomography-and-microscopy-2018-aptm-2018}
Login Host: 129.6.126.220 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both carsten.sachse-at-embl.de as well as the Microscopy Listserver ---------------------------------------------------------------------------
In my lab, a position for in situ cryo-EM in the area of structural cell biology is available. I am looking for a candidate with experience in cryo-ET/EM and/or FIB-SEM for the following project: http://s.embl.org/HD01191
In case you have further questions, please do not hesitate to contact me. Deadline for the position is November 3rd.
Best wishes,
Carsten ____________________________________________________________________________________ Dr. Carsten Sachse Group Leader European Molecular Biology Laboratory Meyerhofstr. 1 69117 Heidelberg Germany email: carsten.sachse-at-embl.de http://www.embl.de/research/units/scb/sachse/ http://www.sachse.embl.de
Login Host: 194.94.44.220 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
A colleague of mine encountered a talk at a recent meeting in which a physician was treating cells or tissues with acridine orange and then bombarding with X-rays and the AO stained cells were killed, while the non-treated cells were not. Their target was osteoclasts to try to prevent bone loss in cancer patients. They seem to have no data on the mechanism, but two hypotheses jump to mind: 1. The AO actually has an absorption/excitation spectrum which includes the wavelength of X-rays (0.01-10nm) and that absorption causes toxicity (free radicals?) 2. The AO is doing something to the cells to make them more sensitive to X-rays (neutralizing endosomes and lysosomes) and it has nothing to do with light absorption by the dye. My question is whether anyone knows if there is data on the behavior of fluorescent dyes like AO in this very short wavelength light spectrum.
Thanks- Dave
Dr. David Knecht Professor , Department of Molecular and Cell Biology University of Connecticut 91 N. Eagleville Rd. U-3125 Storrs, CT 06269-3125 860-486-2200
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Secretary-at-midwestmicroscopy.org as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Winter Meeting of MMMS - Nov, 2017
Message: The winter meeting of the Midwest Microscopy and Microanalysis Society will be held on Nov. 17, 2017 at the Baxter Corporate Headquarters in Deerfield Illinois.
The meeting is Free for M3S members, $20.00 for non-members, $5.00 for students
The theme for this meeting is "Other M: Innovative Microanalysis". Six speakers as listed in the program outline below will discuss a range of contemporary topics in analysis.
Additional information and directions to Baxter is available at the MMMS WWW site.
http://www.midwestmicroscopy.org
MMMS November 17 Program.
8:00 9:00AM Registration, Continental Breakfast will be served
9:00 9:10AM Welcome and Opening Remarks
9:10 10:00AM Surface Microscopy and Microanalysis in an Industrial Research and Development Laboratory: General Electric Global Research Center Vincent Smentkowski, General Electric
10:00 - 10:30AM Mid-IR QCL Spectral Microscopy: Bringing Label-free, Video Rate Chemical Imaging Modalities to the Materials and Life Sciences Jeremy Rowlette, Daylight Solutions
10:30 11:00AM Break & Visit with Vendors
11:00 - 11:45AM From Imaging to Analytics: Getting the Most out of Your X-Rays Will Harris, Zeiss
11:45AM 12:10PM MMMS Business Meeting
12:10 1:30PM Lunch & Visit with Vendors
1:30PM 2:15PM SXES Soft X-ray Emission Spectroscopy Coming Close To Doing the Impossible Vern Robertson, JEOL USA
2:15 2:45PM Renishaw inVia: the Swiss Army Knife of Confocal Raman Systems Tim Prusnick, Renishaw
2:45 - 3:15PM Accelerating Materials Characterization with Machine Learning Karl Husjak, Dravid Group, Northwestern University
3:15 - 3:20PM Closing Remarks
Login Host: 96.65.115.77 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
From otiscatr45k-at-gmail.com Sat Oct 28 02:39:54 2017 Return-Path: {otiscatr45k-at-gmail.com} Received: from gmail.com ([45.115.242.247]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v9S7dr3i021821 for {microscopylistserverarchive6-at-microscopy.com} ; Sat, 28 Oct 2017 02:39:53 -0500 Received: from unknown (165.118.218.216) by mxs.perenter.com with ASMTP; Sat, 28 Oct 2017 02:24:35 -0500 Received: from qrx.quickslick.com ([109.197.211.245]) by mail.gimmicc.net with NNFMP; Sat, 28 Oct 2017 02:22:54 -0500 Received: from unknown (HELO smtp-server1.cfdenselr.com) (Sat, 28 Oct 2017 02:10:53 -0500) by relay-x.misswldrs.com with ASMTP; Sat, 28 Oct 2017 02:10:53 -0500 Received: from smtp.endend.nl ([Sat, 28 Oct 2017 01:55:33 -0500]) by group21.345mail.com with NNFMP; Sat, 28 Oct 2017 01:55:33 -0500 Message-ID: {079175EE.D18CBB73-at-gmail.com}
One of my former histology students sent me this link of "cats hiding in histological specimens". I hope you will forgive me for sharing: http://thebiogeek.com/2016/03/histocats-when-cats-hide-in-histological-specimens/
Thomas E. Phillips, Ph.D Professor of Biological Sciences Curator's Distinguished Teaching Professor Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both unocicrr-at-ornl.gov as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: unocicrr-at-ornl.gov Name: Raymond Unocic
Organization: Oak Ridge National Laboratory
Title-Subject: [Filtered] Postdoctoral Position at ORNL on in situ microscopy and data analytics
Message: There is a postdoc position open in the microscopy group at the Center for Nanophase Materials Sciences Division at Oak Ridge National Laboratory.
If interested please go to ornl.gov/careers and apply for the position number NB50644036 "Gas Cell Microscopy to Probe Interfacial Chemical Reactions"
Brief description is below:
A multidisciplinary research team has been assembled at ORNL to develop and optimize novel electron microscopy imaging techniques and quantitative data analytic methods to understand catalytic processes in situ at the atomic and molecular level. As a Postdoctoral Research Associate, you will design and perform in situ/operando microscopy experiments to determine factors that govern the atomic-scale restructuring of catalysts during gas reactions. You will work with this team to develop advanced and correlative electron microscopy imaging and spectroscopy techniques (e.g., 4D STEM) and quantitative data analytic methods.This post-doc position resides within the Electron and Atom Probe Microscopy Group at the Center for Nanophase Materials Sciences (CNMS). As a core team member, you will work with a diverse group of experimentalists, theorists, and data scientists as part of a project funded through ORNLs Laboratory Directed Research Development (LDRD) Program. Your research will emphasize the characterization of structurally and chemically evolving catalyst nanoparticles using in situ gas cell microscopy, and will be directed toward the correlation of atomic-scale structural changes with catalytic activity. Your work will focus on the use of aberration-corrected scanning transmission electron microscopy (STEM) imaging, 4D STEM, ptycography, and analytical STEM techniques, including electron energy loss spectroscopy (EELS) and energy X-ray dispersive spectroscopy (EDS). You will also advance microscopy data analytic methods to quantify time-resolved images for atomic displacements and atomistic restructuring measurements using ORNLs high performance computing resources.
Best Regards
Ray
Raymond R. Unocic, Ph.D. Oak Ridge National Laboratory Center for Nanophase Materials Sciences One Bethel Valley Road Oak Ridge, TN 37831 unocicrr-at-ornl.gov 865-574-0096
Login Host: 128.219.49.14 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
==============================Original Headers============================== 17, 54 -- From microscopy.listserver-at-gmail.com Tue Oct 31 19:25:42 2017 17, 54 -- Received: from mail-io0-f178.google.com (mail-io0-f178.google.com [209.85.223.178]) 17, 54 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id vA10PgSG031401 17, 54 -- for {microscopy-at-microscopy.com} ; Tue, 31 Oct 2017 19:25:42 -0500 17, 54 -- Received: by mail-io0-f178.google.com with SMTP id n137so2673206iod.6 17, 54 -- for {microscopy-at-microscopy.com} ; Tue, 31 Oct 2017 17:21:21 -0700 (PDT) 17, 54 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 17, 54 -- d=gmail.com; s=20161025; 17, 54 -- h=subject:references:to:from:message-id:date:user-agent:mime-version 17, 54 -- :in-reply-to:content-language:content-transfer-encoding; 17, 54 -- bh=8HfrY32xH0EvVaZCB8FrNjLLYjKkVejEZyeAwIl+NXA=; 17, 54 -- b=Pmp/bN2D8mxqrgFBLbpv6e+EEe+xeNwDlWdUL3GBPnybL39fEt7WAlKT/OXU/fDzWt 17, 54 -- tHsC3wyoJJHgV1UIOCg7pOLIkj3BYAQcy1otlKN9UB0YUm2WWc5i2og8Ax07Si/xQilQ 17, 54 -- LbWq5oHSJEzTC9O/hRF8XzoXDBCskpi8NuQf4Znw4RWIa4z3W/ia7Bypjd/6xrVIXjtl 17, 54 -- iqldQsqrEGd2XzDJ3cQCvVPEQ5QZ9Ys1xUihP2gUOjAQyAcMHXZC1eU2ScLddYacPzj/ 17, 54 -- o4q2Ab8PzokQzlxKsqsr4uDS+jl96zhtGI89BGOOlrE/sObLxSKNeiPQ6IVt0g8hAoit 17, 54 -- W3EA== 17, 54 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 17, 54 -- d=1e100.net; s=20161025; 17, 54 -- h=x-gm-message-state:subject:references:to:from:message-id:date 17, 54 -- :user-agent:mime-version:in-reply-to:content-language 17, 54 -- :content-transfer-encoding; 17, 54 -- bh=8HfrY32xH0EvVaZCB8FrNjLLYjKkVejEZyeAwIl+NXA=; 17, 54 -- b=ddIGDhJV4LeJ7YrfGlooRR4nU9LNGh9sCADkDHeiONIcLwNj2lDLsGUscu75w+Sykm 17, 54 -- vKwGz8i2n/pMPRjtUi2TL3ptZBMSDrctOuQfeVCUQfwEI8B8imtsH0lnVqvFcbjnQ1zs 17, 54 -- WlzvsTRHr4paPPQO+p8tvr2SJLmuI2bz0C2vNo1M/LaCjWrXQ0MzYocyb3/bxF20/mG9 17, 54 -- 5UmjQP2dRpn3mJ87Z1RNxCZiEwDndwxucuep8rsJUttzOCdUEKoCQkaFRxggWCcPPWau 17, 54 -- v/YVn4qVwr5QHUnG7L25j5Fj9B7dJqgSoHPbx992kmALhjcIeHsAKxM2i2dz/byNk5I9 17, 54 -- GKRw== 17, 54 -- X-Gm-Message-State: AMCzsaWWLjyytIsSqSYNRPbSHqiO4gbsLMkk+FJQB2JZQ3SfIaATg0Fg 17, 54 -- vTN8yDg76HF3T68Z63TAhHnTLJxw 17, 54 -- X-Google-Smtp-Source: ABhQp+SMswIb1V3ubDWGXrj9/BM6IQulY0wj2Wy8nSLr58JKprTPFz9Xegcxc7jtRdCzx8sLTZ1htw== 17, 54 -- X-Received: by 10.36.179.11 with SMTP id e11mr5626540itf.136.1509495680878; 17, 54 -- Tue, 31 Oct 2017 17:21:20 -0700 (PDT) 17, 54 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:1ab:a3df:2515:1d7d]) 17, 54 -- by smtp.googlemail.com with ESMTPSA id i63sm1295225ioi.68.2017.10.31.17.21.19 17, 54 -- for {microscopy-at-microscopy.com} 17, 54 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 17, 54 -- Tue, 31 Oct 2017 17:21:20 -0700 (PDT) 17, 54 -- Subject: viaWWW: Postdoctoral Position at ORNL on in situ microscopy and data 17, 54 -- analytics 17, 54 -- References: {201710311327.v9VDRkV9008022-at-microscopy.com} 17, 54 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 17, 54 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} 17, 54 -- X-Forwarded-Message-Id: {201710311327.v9VDRkV9008022-at-microscopy.com} 17, 54 -- Message-ID: {f600d037-83b1-8905-0342-caec61038fe8-at-gmail.com} 17, 54 -- Date: Tue, 31 Oct 2017 19:21:19 -0500 17, 54 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) 17, 54 -- Gecko/20100101 Thunderbird/52.4.0 17, 54 -- MIME-Version: 1.0 17, 54 -- In-Reply-To: {201710311327.v9VDRkV9008022-at-microscopy.com} 17, 54 -- Content-Type: text/plain; charset=UTF-8; format=flowed 17, 54 -- Content-Language: en-US 17, 54 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both stacie-at-ems-secure.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] 2018 EMS Microscopy Academy New Course Schedule Announced
Message: http://www.emsdiasum.com/microscopy/academy/courses/courses.aspx We've updated our calendar for 2018! We have some exciting classes at the EMS Microscopy Academy, including: 1. January 22-26, 2018. Pharmaceutical Microscopy 2. January 30-February 1, 2018 Materials Ultramicrotomy 3. February 13-15, 2018 Automated Rapid Processing 4. February 20-22, 2018 ImmunoGold Silver Staining 5. March 6-8, 2018 Biological SEM 6. March 20-22, 2018 Biological TEM 7. April 3-5, 2018 Material Ultramicrotomy 8. April 10-12, 2018 Cryo SEM 9. April 17-18, 2018 Pharmaceutical Chemical Imaging 10. May 8-10, 2018 Introduction to Microscopy Techniques 11. May 15-17, 2018 X-Ray Microanalysis 12. June 4-8 Cryo Immune and Cryo Sectioning 13. October 8-12, 2018 Cryosectioning and Immunogold 14. October 23-25, 2018 Cryo SEM 15. November 6-8, 2018 Biological TEM 16. November 13-15, 2018 Biological SEM .
Starting Soon Pharmaceutical Microscopy, featuring expert Robert Carlton, January 22 26
Materials Ultramicrotomy, featuring expert Helmut Gnaegi, January 30 February 1
Automated and Rapid Specimen Processing, featuring expert Michael Kostrna, February 13 - 14
We look forward to seeing you in 2018!
Login Host: 73.165.127.32 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
From tammyhoward072ga-at-gmail.com Thu Nov 2 00:20:11 2017 Return-Path: {tammyhoward072ga-at-gmail.com} Received: from gmail.com ([27.50.138.121]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id vA25K8Wl012540 for {microscopylistserverarchive6-at-microscopy.com} ; Thu, 2 Nov 2017 00:20:10 -0500 Received: from unknown (HELO mailout.endmonthnow.com) (Thu, 02 Nov 2017 03:51:59 +0000) by group21.345mail.com with ASMTP; Thu, 02 Nov 2017 03:51:59 +0000 Received: from unknown (HELO snmp.otwaloow.com) (Thu, 02 Nov 2017 03:50:42 +0000) by relay37.vosimerkam.net with NNFMP; Thu, 02 Nov 2017 03:50:42 +0000 Received: from [196.182.171.114] by mmx09.tilkbans.com with QMQP; Thu, 02 Nov 2017 03:48:14 +0000 Message-ID: {97FC2846.1AD25EAA-at-gmail.com}
X-from: erwrigh-at-emory.edu
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both erwrigh-at-emory.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: erwrigh-at-emory.edu Name: Elizabeth Wright
Organization: Emory University
Title-Subject: [Filtered] Postdoctoral fellow positions - cryo-EM of host-pathogen systems
Message: NIH-funded postdoctoral positions to study either virus assembly or bacterial appendages and secretion systems are available in the Wright lab (http://electronmicroscopy.emory.edu). Major research efforts in the lab are focused on 3D structure/function relationships between host cells and viruses or bacteria. Areas of particular interest include cryo-electron tomography of bacteria, bacteria bacteriophage interactions, paramyxoviruses, and HIV-1. Technology development interests are in the areas of correlative microscopy and phase plate cryo-microscopy.
Representative publications applicable to the projects associated with the position(s): 1. C. M. Hampton, J. D. Strauss, Z. Ke, R. S. Dillard, J. E. Hammonds, E. Alonas, T. M. Desai, M. Marin, R. E. Storms, F. Leon, G. B. Melikyan, P. J. Santangelo, P. W. Spearman, and E. R. Wright. Correlated Fluorescence Microscopy And Cryo-Electron Tomography Of Virus-Infected And Transfected Mammalian Cells. Nature Protocols. 2017: 12 (1): 150-167. 2. C. C. Stobart, C. A. Rostad, Z. Ke, R. S. Dillard, C. M. Hampton, J. D. Strauss, H. Yi, A. L. Hotard, J. Meng, R. J. Pickles, K. Sakamoto, S. Lee, M. G. Currier, S. M. Moin, B. S. Graham, M. S. Boukhvalova, B. E. Gilbert, J. C. G. Blanco, P. A. Piedra, E. R. Wright, and M. L. Moore. "A Live-Attenuated RSV Vaccine With Increased Incorporation Of Pre-Fusion F Exhibits Enhanced Thermal Stability And Immunogenicity." Nature Communications. 2016: 7: 13916. 3. C. K. Ellison, J. Kan, R. S. Dillard, D. T. Kysela, C. M. Hampton, Z. Ke, E. R. Wright, N. Biais, A. B. Dalia, and Y. V. Brun. Obstruction Of Pilus Retraction Stimulates Bacterial Surface Sensing. Science. 2017: 358 (6362): 535-538. 4. R. C. Guerrero-Ferreira, P. H. Viollier, B. Ely, J. S. Poindexter, M. Georgieva, G. J. Jensen, and E. R. Wright. Alternative Mechanism For Bacteriophage Adsorption To The Motile Bacterium Caulobacter crescentus. Proc Natl Acad Sci U.S.A. 2011: 108(24): 9963-9968. The wet laboratory is within the Division of Pediatric Infectious Diseases and is fully equipped for all aspects of microbiology, molecular biology, molecular virology, and protein biochemistry. The Wright lab is equipped with a JEOL JEM-2200FS 200 kV FEG TEM with an in-column energy filter, phase plate system, Direct Electron DE-20 direct electron detector, and Gatan 4kx4k CCD camera. This instrument will also serve as a feeder microscope to FSU and other cryo-EM consortia with Titan Krios cryo-microscopes. For cryo-CLEM development and experiments, there is a Leica cryo-CLEM system and access to multiple live-cell imaging platforms. The laboratory also has an FEI Vitrobot, a Gatan CP3 and a manual grid plunge freezer; and a Leica cryo-ultramicrotome. The group has access to all the resources available in the Emory University EM Core Facility, to include: a JEOL JEM-1400 120 kV TEM with Gatan 2kx2k CCD camera; a Bal-Tec high-pressure freezer; and Leica freeze substitution system.
Qualifications: Candidates should have a PhD in microbiology, virology, structural biology, or other related scientific discipline. Candidates should have an interest in structural biology and microbiology; excellent communication skills; and enjoy working as part of a dynamic and collaborative research team. Applicants should include a cover letter describing research experience and interests, curriculum vitae, and names and contact information for three references. Applications will be reviewed as they are received and until the position is filled.
Best,
Liz ---- Elizabeth R. Wright, PhD Associate Professor Emory University (404) 727-4665 erwrigh-at-emory.edu
Login Host: 170.140.104.11 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Sergio.Sanchez5-at-Honeywell.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Message: All, My group is in the process of decommissioning our JEM-840A SEM but would like to see if anyone is in need of it (parts or the entire instrument) before we will be obligated to dispose of it since it takes up valuable lab space.
If interested, please contact me:
Sergio I. Sanchez R&D Manager Microscopy and Metallurgy Honeywell UOP 25 E. Algonquin Rd., Des Plaines, IL 60017 Office: +1 847.391.1196 Sergio.Sanchez5-at-Honeywell.com
Login Host: 199.64.6.149 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
From gunnclar77m-at-gmail.com Fri Nov 3 07:30:40 2017 Return-Path: {gunnclar77m-at-gmail.com} Received: from gmail.com ([45.115.242.246]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id vA3CUcep022792 for {microscopylistserverarchive6-at-microscopy.com} ; Fri, 3 Nov 2017 07:30:39 -0500 Received: from relay.2yahoo.com ([86.68.59.2]) by mts.locks.grgtween.net with LOCAL; Fri, 03 Nov 2017 14:17:23 +0200 Message-ID: {728E20F4.05D9D84D-at-gmail.com}
X-from: andy.stewart-at-ul.ie
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both andy.stewart-at-ul.ie as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: andy.stewart-at-ul.ie Name: Andrew Stewart
Organization: University of Limerick
Title-Subject: [Filtered] Electron crystallography School
Message: Dear all,
We would like to bring to your attention the deadline (30th of November 2017) for the International school of Crystallography 2018 on the topic of Electron Crystallography.
The Course intends to review the traditional as well as the modern methods of electron crystallography; it will be divided into three major fields: 1. provide a strong background on crystallography in general and electron crystallography in particular. 2. introduce students to state-of-art techniques of electron crystallography including experimental techniques, data acquisition, and data processing as well as to supporting technologies such as spectroscopy. 3. cover different approaches for structure analysis.
The school will take place in Erice, Italy, at the Ettore Majorana Foundation and center for scientific culture. Please apply here: http://crystalerice.org/2018/.
Application Deadline: 30th of November 2017
With kind regards from the course directors, Joke Hadermann, Lukas Palatinus, and Andy Stewart.
Login Host: 193.1.100.60 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
From pedugjuf5b-at-gmail.com Mon Nov 6 18:03:22 2017 Return-Path: {pedugjuf5b-at-gmail.com} Received: from gmail.com ([45.115.242.244]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id vA703Lg4003720 for {microscopylistserverarchive6-at-microscopy.com} ; Mon, 6 Nov 2017 18:03:21 -0600 Received: from mail.gimmicc.net ([173.147.207.141]) by qrx.quickslick.com with ASMTP; Tue, 07 Nov 2017 01:46:54 +0200 Received: from unknown (HELO relay-x.misswldrs.com) (Tue, 07 Nov 2017 01:34:15 +0200) by mx03.listsystemsf.net with QMQP; Tue, 07 Nov 2017 01:34:15 +0200 Received: from mx03.listsystemsf.net ([172.220.144.78]) by relay.2yahoo.com with SMTP; Tue, 07 Nov 2017 01:14:37 +0200 Received: from unknown (61.143.201.177) by webmail.halftomorrow.com with QMQP; Tue, 07 Nov 2017 00:54:54 +0200 Received: from unknown (HELO mailout.endmonthnow.com) (Tue, 07 Nov 2017 00:35:14 +0200) by mx.reskind.net with QMQP; Tue, 07 Nov 2017 00:35:14 +0200 Message-ID: {C1313177.43E8C70C-at-gmail.com}
Hi, all
I know that more and more of us are working with nanomaterials. DeGruyter just published a new textbook (also great for those of you in industry) for which a colleague of mine was editor: "Nano-safety: What We Need to Know to Protect Workers" (ISBN-10: 3110373750)
I was honored enough to be asked to write the intro chapter on Nanotech, Role of the government in Nanosafety, a discussion of the Market, and Educational programs related to nanosafety. (Caveat: No commercial interest). The more detailed chapters were written by a number of pioneers in Nanosafety, including Dr. Walt Trybula and our own Deb Newbury.
Details are at Amazon.com
Good hunting! Barbara Foster, President & Chief Consultant Microscopy/Microscopy Education ... "Education, not JustTraining" 7101 Royal Glen Trail, Suite A - McKinney, TX 75070 P: 972-924-5310 W: www.MicroscopyEducation.com Microscopy/Microscopy Education is a division of The Microscopy & Imaging Place, Inc.
NEW! Getting involved in Raman or FTIR? MME is now offering courses in these areas specifically for microscopists! Call today for details.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both gary-at-gaugler.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: gary-at-gaugler.com Name: Gary Gaugler
Organization: Microtechnics
Title-Subject: [Filtered] BSD for Zeiss Supra 40 - again
Message: I'm giving one last attempt to find a decent backscatter detector for Supra 40. This has been going on for two years with no success.
Zeiss sent a nice Deben Centaurus scintillator unit but it did not fit the chamber--total length was 2cm too short to reach the pole piece. No reasonable option to fix this.
Zeiss won't send a demo QBSD AsB detector to examine without an installation purchase order. Declined. No pictures nor any instructions to figure out where the thing will go and which port would be used.
Anyone have any thoughts about how to get a BSD for Supra 40 and retire the KE Type 211 QBSD? Integration into SmartSEM would be highly desirable but if not available, some fashion of external input might work.
Gary Gaugler, PhD Microtechnics
Login Host: 104.220.49.227 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both bonnie_salyer-at-tedpella.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Message: Live and work in one of the most beautiful areas in California Redding, CA. Ted Pella Inc. is seeking a Materials Science Product Specialist, who will be responsible product development and sales/marketing activities related to our SEM, Materials Science, Forensic and AFM product lines. Exciting times for this position as we recently acquired the assets of a product lines for semi-conductor industry. Must have BS in Materials Science, 5 years hands on SEM instrument use and specimen prep experience, and 2 years TEM instrument use and specimen prep experience. To apply email letter of interest, resume and salary requirements. For complete job posting see our website www.tedpella.com or LinkedIn.
Login Host: 12.7.209.242 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both kuryu-at-rockefeller.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Subptimal HPF cooling rates and reparation?
Message: Dear List,
We have been experiencing suboptimal cooling rates of High Pressure Freezer (HFP). It used to be easily 20,000 K degree/sec or higher. Now it is ~18,000 K degree/sec at the best. I couldn't find a written specification of the cooling rates. Done anyone have it? Our experience indicates that quality of data become questionable, if it gets 17,000 K degree/sec or lower. Has anyone experienced something similar? Has anyone been able to repair HPF from the condition like this and get 20,000 K degree/sec or higher again? If so, can you share how you did it? Many thanks,
Regards, Hiro
Login Host: 129.85.110.225 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both lavoie-at-uw.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: lavoie-at-uw.edu Name: Ellen Lavoie
Organization: Univ Washington
Title-Subject: [Filtered] Gatan Solarus Plasma Cleaner Message: Although I think I already know the answer to this question thought I'd get some more thoughts. I've been trying to get hold of someone at Gatan with no luck so to you all I ask...I have a student who would like to do a "hydrogen passivation" experiment which is basically bombarding a diamond sample with H2 only in a vacuum. I'm trying to determine if the Solarus can be used for H2 gas only (typically a mixture only is used) and if any longer than a couple of minutes might be a problem. Thoughts?? My, and a chemistry colleague are guessing, no, it will be a problem. Please send me a personal email. Cheers, Ellen Login Host: 128.95.174.10 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Ursel.Bangert-at-ul.ie as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: Ursel.Bangert-at-ul.ie Name: Ursel Bangert
Organization: Bernal Institute, University of Limerick, Ireland
Title-Subject: [Filtered] Post Doctoral Researcher in Electron Microscopy
Message: A position for a Post Doctoral Researcher in Electron Microscopy is available in the Bernal Institute at the University of Limerick (UL), Ireland, as part of a US-Ireland partnership project in collaboration with groups at the University of Nebraska, USA and Queens University Belfast, Northern Ireland. The overall aim of the project is to develop routes for engineering and control of ferroelectric domain walls and exploring their structure and electronic properties with the intention to exploit them in advanced nanoelectronic devices. ULs role is to carry out high-resolution electron microscopy and spectroscopy studies. An FEI Titan Themis TEM (double corrected, monochromated, with analytical, i.e., Super-EDX and Quantum EELS capacities, and equipped with in-situ holders and a Gatan K2 detector) will be used to assess and reveal nano- and electronic-structure of ferroelectric materials grown in this project (including in-situ studies). For further details of the position please follow the link http://www.ul.ie/hrvacancies/; the job ID is 024648.
Login Host: 193.1.100.69 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both kuryu-at-rockefeller.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Subptimal HPF cooling rates and reparation
Message: Dear List,
Apology for typo in the earlier communication.
We have been experiencing suboptimal cooling rates of High Pressure Freezer (HFP). It used to be easily 20,000 K degree/sec or higher. Now it is ~18,000 K degree/sec at the best. I have been searching for a written specification of the cooling rate, but to no avail. I wonder anyone has it. Our experience indicates that quality of data became questionable if it got 17,000 K degree/sec or lower. Has anyone experienced something similar? Has anyone been able to repair HPF from the condition like this to 20,000 K degree/sec or higher again? If so, would you mind sharing how you did it?
Many thanks,
Regards, Hiro Uryu
Login Host: 129.85.110.225 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
Hooke College of Applied Sciences, located in Westmont, IL, is offering a Scanning Electron Microscopy short course December 10-15, 2017. In addition to lectures, this course emphasizes hands-on training using state-of-the-art equipment.
For further SEM training details and registration information, please follow the link below:
We are advertising for 2-year postdoctoral position in LA-ICPMS at University of Tasmania. The advertisement is below. If you know of anyone who may be interested, please pass it on. Please contact Leonid for any questions, see his contact details in the ad below.
Many thanks, Karsten
The position is a fixed-term 2-year appointment funded jointly by Laurin Technic and CODES Analytical Laboratories. The position will involve conducting research aimed at developing new LA-ICPMS techniques and understanding the fundamentals of laser ablation, assisting with operating one LA-ICPMS unit within CODES Analytical Laboratories (a quadrupole ICPMS and an excimer laser microprobe) and assisting with performing analyses for external users. To be considered, you will have: PhD, or equivalent, in geochemistry. Demonstrated ability to conduct research and publish research outcomes in scholarly peer-review journals Demonstrated experience working with LA-ICPMS instrumentation. Demonstrated ability to write reports and deliver scientific presentations Demonstrated high-level written and oral communication skills. Appointment to this role will be at Academic Level A and will have a total remuneration package of up to $102,848 comprising base salary within the range of $65,798 to $87,905 plus 17% superannuation. For further information about this position please contact Professor Leonid Danyushevsky, Head of Earth Science, L.Dan-at-utas.edu.au / 03 6226 2469. To apply, please visit https://jobs.utas.edu.au/psp/ps/EMPLOYEE/HRMS/c/HRS_HRAM.HRS_APP_SCHJOB.GBL?FOCUS=Applicant&SiteId=1 and search for position No. 1476. Your application must as a minimum include your resume, a cover letter and your responses to the position selection criteria. The position description, including the selection criteria, is available on the above website.
Dr Karsten Goemann Senior Research Fellow, Scanning Electron Microscopy & X-Ray Microanalysis Central Science Laboratory, University of Tasmania Mail: Private Bag 74, Hobart TAS 7001, Australia Deliveries: Chemistry Building Loading Bay, Dobson Road, Sandy Bay TAS 7005, Australia Location: Rooms 254-256, Chemistry Building, Dobson Road, Sandy Bay TAS 7005, Australia T: +61 (0)3 6226 2146 | F: +61 (0)3 6226 2494 | M: +61 (0)407 101 990 www.utas.edu.au/research/central-science-laboratory CRICOS 00586B
University of Tasmania Electronic Communications Policy (December, 2014). This email is confidential, and is for the intended recipient only. Access, disclosure, copying, distribution, or reliance on any of it by anyone outside the intended recipient organisation is prohibited and may be a criminal offence. Please delete if obtained in error and email confirmation to the sender. The views expressed in this email are not necessarily the views of the University of Tasmania, unless clearly intended otherwise.
==============================Original Headers============================== 11, 46 -- From karsten.goemann-at-utas.edu.au Fri Nov 10 20:37:19 2017 11, 46 -- Received: from au-smtp-delivery-109.mimecast.com (au-smtp-delivery-109.mimecast.com [180.189.28.109]) 11, 46 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id vAB2bHV2006381 11, 46 -- for {microscopy-at-microscopy.com} ; Fri, 10 Nov 2017 20:37:19 -0600 11, 46 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=utas.edu.au; s=mimecast20170703; t=1510367609; h=from:subject:date:message-id:to:cc:mime-version:content-type:content-transfer-encoding; bh=h7GCN29xMQjAGcUrdq4uoPjzbiBbax3Mf9onAwf1I7U=; b=PhiDfPBNpaxyIuYkyuTosKqk2vVCfNH9WSocQC4TV+IKT4K7lP4qjmWxn+TAhq6tOGj2+wx9VvMDgwdML4UF6qCsk++d1IGUAc1c1UMEW7CZvw53utsnA5IoVBYC8IoSInInrjcqeoJ+oyY3STEHZYxJzr8mzVnDHh8jqcKWFl1syLaYfPj7D0Ay44rkAcTJx/G90/oWu/7ZPYQQ/fhrurud85LRTgQehpbc9nmvnnf6JkCo4U/mlLAasRfNTNUR8p+p/EJXVWutRP16j0kQdBxguSfhH3bEy1qGc41p4hM/Y3h4AMpvFbM7UUZelS5M8GkLSrTZCqWsxKHjooN84A== 11, 46 -- X-IronPort-AV: E=Sophos;i="5.44,377,1505743200"; 11, 46 -- d="scan'208";a="85061673" 11, 46 -- Received: from mx4.utas.edu.au (mx.utas.edu.au [131.217.6.14]) by 11, 46 -- au-smtp-1.mimecast.com with ESMTP id au-mta-23-yncxqW0yN92N2yMA0FLugw-1; 11, 46 -- Sat, 11 Nov 2017 13:33:26 +1100 11, 46 -- Received: from unknown (HELO smtp-node2.its.utas.edu.au) ([10.10.3.73]) 11, 46 -- by ironport4-inside.its.utas.edu.au with ESMTP; 11 Nov 2017 13:33:14 +1100 11, 46 -- Received: from MBX7.utas.ad.internal (MBX7.its.utas.edu.au [10.10.5.234]) 11, 46 -- by smtp-node2.its.utas.edu.au (8.14.4/8.14.9/0) with ESMTP id vAB2XEJd032054 11, 46 -- (version=TLSv1/SSLv3 cipher=AES256-SHA256 bits=256 verify=FAIL) 11, 46 -- for {Microscopy-at-microscopy.com} ; Sat, 11 Nov 2017 13:33:14 +1100 11, 46 -- Received: from MBX12.utas.ad.internal (10.10.5.229) by MBX7.utas.ad.internal 11, 46 -- (10.10.5.234) with Microsoft SMTP Server (TLS) id 15.0.1320.4; Sat, 11 Nov 11, 46 -- 2017 13:33:14 +1100 11, 46 -- Received: from MBX12.utas.ad.internal ([fe80::a07e:f53a:919e:60fb]) by 11, 46 -- MBX12.utas.ad.internal ([fe80::a07e:f53a:919e:60fb%17]) with mapi id 11, 46 -- 15.00.1320.000; Sat, 11 Nov 2017 13:33:14 +1100 11, 46 -- From: Karsten Goemann {karsten.goemann-at-utas.edu.au} 11, 46 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 11, 46 -- CC: Leonid Danyushevsky {l.dan-at-utas.edu.au} 11, 46 -- Subject: 2-year postdoctoral position in LA-ICPMS at University of Tasmania 11, 46 -- Thread-Topic: 2-year postdoctoral position in LA-ICPMS at University of 11, 46 -- Tasmania 11, 46 -- Thread-Index: AQHTWpVsQShl7L8tr0GdO5j4FAaTMA== 11, 46 -- Date: Sat, 11 Nov 2017 02:33:13 +0000 11, 46 -- Message-ID: {2CC6816C-99CC-4E9D-9A3B-21D942EAD02B-at-utas.edu.au} 11, 46 -- Accept-Language: en-AU, en-US 11, 46 -- Content-Language: en-US 11, 46 -- X-MS-Has-Attach: 11, 46 -- X-MS-TNEF-Correlator: 11, 46 -- x-mailer: Apple Mail (2.3445.4.7) 11, 46 -- x-ms-exchange-messagesentrepresentingtype: 1 11, 46 -- x-ms-exchange-transport-fromentityheader: Hosted 11, 46 -- x-originating-ip: [118.208.211.229] 11, 46 -- x-utas-disclaimer: True 11, 46 -- Content-ID: {A6360DF335FF804BA2BFEDBCA8A723B5-at-tempsmtp.utas.edu.au} 11, 46 -- MIME-Version: 1.0 11, 46 -- X-MC-Unique: yncxqW0yN92N2yMA0FLugw-1 11, 46 -- Content-Type: text/plain; charset=WINDOWS-1252 11, 46 -- Content-Transfer-Encoding: 8bit 11, 46 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id vAB2bHV2006381 ==============================End of - Headers==============================
From doripama101ov-at-gmail.com Sat Nov 11 06:19:43 2017 Return-Path: {doripama101ov-at-gmail.com} Received: from gmail.com ([45.115.242.244]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id vABCJghp028538 for {microscopylistserverarchive6-at-microscopy.com} ; Sat, 11 Nov 2017 06:19:43 -0600 Received: from unknown (34.30.88.92) by webmail.halftomorrow.com with ASMTP; Sat, 11 Nov 2017 11:07:12 -0100 Received: from mail.naihautsui.co.kr ([Sat, 11 Nov 2017 10:50:52 -0100]) by mail.webhostings4u.com with ASMTP; Sat, 11 Nov 2017 10:50:52 -0100 Message-ID: {417A9EC5.A052BE1C-at-gmail.com}
X-from: christopher.bleck-at-nih.gov
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both christopher.bleck-at-nih.gov as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: christopher.bleck-at-nih.gov Name: Christopher Bleck
Organization: National Institutes of Health, NHLBI Electron Microscopy Core Facility Title-Subject: [Filtered] Job Opening / Electron Microscopy Contractor
Message: This position is within the National Heart, Lung, and Blood Institute at The National Institutes of Health (NIH), a part of the U.S. Department of Health and Human Services.
The Electron Microscopy core places a strong emphasis on creating an environment where scientists and physician-scientists can work together on disease-specific issues using the most appropriate approaches available. There are great interactions with a wide range of independent research groups, and the position offers exceptional opportunities for interdisciplinary collaboration within and outside of the NIH.
If interested, see the full job description and apply at the following location: https://www.idealist.org/en/government-job/1277d9e4dee1437d9a52c7cc3511d09d-electron-microscopy-contractor-national-heart-lung-and-blood-institute-bethesda?
Login Host: 156.40.233.194 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jhyun-at-gatan.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: jhyun-at-gatan.com Name: John Hyun
Organization: Gatan
Title-Subject: [Filtered] European EELS & EFTEM School at Graz University
Message: European EELS & EFTEM School at Graz University
Graz University of Technology, Graz, Austria 6 9 February 2018
This four-day, hands-on laboratory school will take you step-by-step through the acquisition and analysis of EFTEM and STEM-EELS data. The school will utilize the state-of-the-art facilities and faculty of EELS experts at FELMI-ZFE. The featured microscope system that will be used is a monochromated probe-corrected (S)TEM with a DualEELS system.
EELS experts will train you in the latest EELS and EFTEM instruments and software and present and demonstrate fundamental principles and methods essential to acquire optimal EELS spectra, STEM-EELS spectrum images, energy-filtered images, and elemental maps.
The ultimate goal is to provide you with the training and knowledge needed to acquire your best EELS and EFTEM results.
Seating is very limited. We encourage you to register now. If you have any questions, please contact Dr. Gerald Kothleitner, Graz University: gerald.kothleitner-at-felmi-zfe.at.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both wfschneider-at-wisc.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: wfschneider-at-wisc.edu Name: Bil Schneider
Message: I'm searching for a spare filter housing assembly for our Gatan PanaCL detector for the SEM. I've called Gatan a few times in an attempt to procure the part but they never get back to me. If you have a new or used filter housing assembly for the PanaCl detector or know where I might find one please contact me. I cannot find a part number, but it is the housing that has four one inch openings for standard circular optical filters.
Thank You, Bil Schneider SEM Manager UW Geosciences University of Wisconsin, Madison wfschneider-at-wisc.edu
Login Host: 144.92.207.84 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both raffaella.carzaniga-at-crick.ac.uk as well as the Microscopy Listserver ---------------------------------------------------------------------------
Organization: The Francis Crick Institute London (UK)
Title-Subject: [Filtered] few days left to apply for a position at the Electron Microscopy Science Technology platform - The Francis Crick Institute - London (UK)
Message: Dear colleagues,
Last few days to apply to be part of the EM stp team at The Francis Crick Institute in London. Role title: Senior Laboratory Research Scientist (Electron Microscopy) Location: The Francis Crick Institute, Midland Road, London Contract: Fixed-term (4 years), Full time Salary: Competitive with benefits, subject to skills and experience Vacancy ID: 6204 The Role To take on this position, you will have a degree and PhD in the biological sciences, expertise in electron microscopy (EM) and the application of EM to biomedical research questions, and enthusiasm for working in a facility environment. Expertise in standard resin-embedding of cells and tissues, ultramicrotomy, scanning and transmission EM are essential. Expertise in advanced sample preparation techniques (such as cryo-preparation and correlative workflows), advanced electron microscopes (Serial Block Face SEM, Focused Ion Beam SEM, cryo-EM), other imaging modalities (light and X-ray microscopy), and image analysis are desirable. This post offers an unparalleled opportunity to apply your microscopy skills to exciting and innovative research across biomedical disciplines, with access to cutting edge instrumentation, and the chance to develop completely new imaging techniques and technologies for direct application to biomedical research. The role reports to the Deputy Head of the EM STP. If you are interested in applying for this role, please apply via our website https://www.jobs.crick.ac.uk The closing date for applications is 16 November 2017 at 23:30 pm.
Best wishes Raffa Raffaella Carzaniga PhD Deputy Head of Electron Microscopy The Francis Crick Institute 1 Midland Road London NW1 1AT UK T: +44 (0) 20379 61732 E: Raffaella.carzaniga-at-crick.ac.uk W: www.crick.ac.uk
Login Host: 90.209.15.62 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
==============================Original Headers============================== 17, 54 -- From microscopy.listserver-at-gmail.com Sun Nov 12 13:39:30 2017 17, 54 -- Received: from mail-pg0-f68.google.com (mail-pg0-f68.google.com [74.125.83.68]) 17, 54 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id vACJdUDp019831 17, 54 -- for {microscopy-at-microscopy.com} ; Sun, 12 Nov 2017 13:39:30 -0600 17, 54 -- Received: by mail-pg0-f68.google.com with SMTP id s2so11107958pge.10 17, 54 -- for {microscopy-at-microscopy.com} ; Sun, 12 Nov 2017 11:35:47 -0800 (PST) 17, 54 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 17, 54 -- d=gmail.com; s=20161025; 17, 54 -- h=subject:references:to:from:message-id:date:user-agent:mime-version 17, 54 -- :in-reply-to:content-language:content-transfer-encoding; 17, 54 -- bh=bacYNsW2m5uORBzsUhRrdox/HxgGk0SXDGRI+o1aWNA=; 17, 54 -- b=Y5B0jRzDPUAeejA0sXVjlQv4p1+ds7he30doCiZ/wsHBR6Wexhtp+NHy1drp9iz9ev 17, 54 -- UXGK7iICfUwEqTWhLwiMo8G1hGekMoS50++KiLt8O+NAOJwEK4ZZXA/qIvXTyA9aOMPS 17, 54 -- HI3uf9jlrMP74LBdVGHMzUUXBTic2Hg5dBwRYQVECsdoBYJ/p2W3aYVcRVaqbnfXXbyT 17, 54 -- j5tJ92OT9ChB6n3fmOxO33eL0qkx4RW263Zc2ZsLNL9S/3KhXfUGgnROsx6/AJPvKKil 17, 54 -- /Ssxmsz1giIQgJuijxjgNSKPI2DMH34V3ZAvtjj1nFAMtmDcEE4cGCz6sTI+nK9mQFCB 17, 54 -- XsmQ== 17, 54 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 17, 54 -- d=1e100.net; s=20161025; 17, 54 -- h=x-gm-message-state:subject:references:to:from:message-id:date 17, 54 -- :user-agent:mime-version:in-reply-to:content-language 17, 54 -- :content-transfer-encoding; 17, 54 -- bh=bacYNsW2m5uORBzsUhRrdox/HxgGk0SXDGRI+o1aWNA=; 17, 54 -- b=bZfvF7qVSUgPJn7IBeUurKbAH9Pt4DhNAuxIW+5L8zpvrsej50/Kds5fWByUIn6nCC 17, 54 -- b5mp5sexJuj5k+jEuXGRIik9nvICNpNZ+N7C2gX2blUFOPcdpUqIcAHykpqtU/SWfJZf 17, 54 -- EkW/A6agHPmvLGoy5iAdsFviFHXRUJArXGPe1bo9K9rPBnu/xvAdFZ0ZSKDffScBS4om 17, 54 -- GzJnFCrczn7FVz5T5JL8lr5dLNwVzsFzBBfk5EBVrGn4+TFyVRsZZNwGk5+AOGPLkwPo 17, 54 -- jm3/5a9kJsLa+qK0jJIeiyY43DfEfPtCMbgleXQ3yJ+qwBUtWQj1HkUVNEqxMpWcuIxL 17, 54 -- tg8g== 17, 54 -- X-Gm-Message-State: AJaThX6fIfa/9XGAsHY9b7fKsg6wv/P1UwKcnLnvgLww1qa4NBuqvQQf 17, 54 -- gtDe08qF5YA7dCVC0nc0x7md8w== 17, 54 -- X-Google-Smtp-Source: AGs4zMZOfhQRhmELYIa5YUHiNLCCVQk40At7U8MzhbPztU8zmpYL6QJR0pBIl2du491nDj/oOGa5gg== 17, 54 -- X-Received: by 10.98.87.138 with SMTP id i10mr7395028pfj.185.1510515346866; 17, 54 -- Sun, 12 Nov 2017 11:35:46 -0800 (PST) 17, 54 -- Received: from Nestors-MacBookAir-Pro-2014-ElCapitan-OSX-1163.local ([118.189.143.35]) 17, 54 -- by smtp.googlemail.com with ESMTPSA id u76sm29871765pfk.181.2017.11.12.11.35.45 17, 54 -- for {microscopy-at-microscopy.com} 17, 54 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 17, 54 -- Sun, 12 Nov 2017 11:35:46 -0800 (PST) 17, 54 -- Subject: viaWWW:position at the Electron Microscopy Science Technology 17, 54 -- platform - The Francis Crick Institute - London (UK) 17, 54 -- References: {201711121701.vACH13Dd013808-at-microscopy.com} 17, 54 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 17, 54 -- From: Microscopy Listserver {microscopy.listserver-at-gmail.com} 17, 54 -- X-Forwarded-Message-Id: {201711121701.vACH13Dd013808-at-microscopy.com} 17, 54 -- Message-ID: {9c09de57-11f9-d0a0-5925-52caa6e8eb9e-at-gmail.com} 17, 54 -- Date: Mon, 13 Nov 2017 03:35:43 +0800 17, 54 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) 17, 54 -- Gecko/20100101 Thunderbird/52.4.0 17, 54 -- MIME-Version: 1.0 17, 54 -- In-Reply-To: {201711121701.vACH13Dd013808-at-microscopy.com} 17, 54 -- Content-Type: text/plain; charset=windows-1252; format=flowed 17, 54 -- Content-Language: en-US 17, 54 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both kuryu-at-rockefeller.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Electron Microscopist Position Available
Message: Dear List,
The Rockefeller University, a premier biomedical research institution, seeks a Research Support-at-Associate or Specialist to join our Electron Microscopy Resource Center (EMRC).
The EMRC provides state-of-the-art electron microscopy support for analysis of a wide variety of biological samples, including viruses, bacteria, insects, animal tissue as well as cultured cells and isolated cellular components for structural analyses or immuno-electron microscopy. The EMRC is equipped with three transmission electron microscopes, a conventional and a serial block-face imaging scanning electron microscope, and a high-pressure freezing and a freeze-substitution unit. (http://www.rockefeller.edu/emrc/)
The Research Support Associate/Specialist will participate in all of the EMRCfs daily operations, including maintenance, upkeep and use of the electron microscopes and associated equipment, ordering supplies, interacting with vendors, and administrative support for office duties, including center billing. The position also entails specimen preparation, including negative staining, ultrathin sectioning, and immunolabeling, operation of the microscopes and associated equipment, training users, as well as consulting scientists on the design of experiments, data processing/analysis, interpretation of results, and informing users on the latest methodology through familiarity with relevant literature.
The successful candidate will have an M.S./Ph.D. degree or equivalent background in biology, bioengineering or a related field and must have a minimum of 5 years of hands-on experience in electron microscopy. A strong background in computation would be a plus. Must have strong communication skills, and the ability to work collaboratively in a team as well as independently on a wide variety of research projects. Must be detail-oriented, focused, and highly motivated.
We offer a competitive salary, comprehensive benefits, and a collegial work environment. To apply to this job, click the following URL, click on 'staff opportunitiesf and enter keyword eIRC20449f or 'Electron Microscopist': http://www.rockefeller.edu/hr/career.php
The Rockefeller University is an Affirmative Action/Equal Employment Opportunity/VEVRAA employer.
Kunihiro Uryu, Ph.D. Director, Electron Microscopy Resource Center
Login Host: 129.85.110.225 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
Two positions are available in the electron microscopy facility at the MRC Laboratory of Molecular Biology in Cambridge
One position is for an EM engineer, the other for a senior research support assistant. The two positions are part of the team that manage, maintains and develops the facility, supports users, and gets involved in collaborative projects aimed at improving cryo-EM.
The LMB has a vibrant cryo-electron microscopy community. The facility will soon have three Titan Krios, two Polara, two other FEG microscopes, a Scios dual-beam and lower-end microscopes. It has approximately 150 active users applying electron cryomicroscopy and cryo-electron tomography to study important biological problems. There are research programmes aimed at the development of new hardware and software for cryo-electron microscopy.
Further details are available here:
EM engineer position: https://mrc.tal.net/vx/mobile-0/appcentre-ext/brand-3/candidate/so/pm/4/pl/1/opp/702-Electron-Microscopy-EM-Engineer-Structural-Studies-EM-Facility-LMB-702/en-GB
EM research support assistant position: https://mrc.tal.net/vx/mobile-0/appcentre-ext/brand-3/candidate/so/pm/4/pl/1/opp/691-Research-Support-Officer-Structural-Studies-Electron-Microscopy-Facility-LMB-691/en-GB
==============================Original Headers============================== 6, 19 -- From john.briggs-at-embl.de Mon Nov 13 06:16:02 2017 6, 19 -- Received: from ironport1.embl.de (ironport1.embl.de [194.94.44.73]) 6, 19 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id vADCG14A006345 6, 19 -- for {Microscopy-at-microscopy.com} ; Mon, 13 Nov 2017 06:16:02 -0600 6, 19 -- Received: from lxmail01-vm.embl.de (HELO mail.embl.de) ([10.11.5.101]) 6, 19 -- by ironport1.embl.de with ESMTP; 13 Nov 2017 13:12:20 +0100 6, 19 -- Received: (qmail 4956 invoked by uid 107); 13 Nov 2017 12:12:19 -0000 6, 19 -- Received: from unknown (HELO jbriggs-tdock-1.lmb.internal) (briggs-at-131.111.85.79) 6, 19 -- by lxmail01-vm.embl.de with ESMTPA; 13 Nov 2017 12:12:19 -0000 6, 19 -- From: John Briggs {john.briggs-at-embl.de} 6, 19 -- Content-Type: text/plain; charset=us-ascii 6, 19 -- Mime-Version: 1.0 (Mac OS X Mail 10.3 \(3273\)) 6, 19 -- Subject: Two job openings in Electron Microsopy 6, 19 -- Message-Id: {5A00A020-C38F-4CAE-9B05-E497EC9717D0-at-embl.de} 6, 19 -- Date: Mon, 13 Nov 2017 12:12:19 +0000 6, 19 -- To: Microscopy-at-microscopy.com 6, 19 -- X-Mailer: Apple Mail (2.3273) 6, 19 -- Content-Transfer-Encoding: 8bit 6, 19 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id vADCG14A006345 ==============================End of - Headers==============================
From prvs=484267805=laism-at-ascpl.com.my Tue Nov 14 00:04:39 2017 Return-Path: {prvs=484267805=laism-at-ascpl.com.my} Received: from BKDC-DMZ-ESA-02.idc.bsc.rw ([197.243.16.10]) by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id vAE64UT8003799; Tue, 14 Nov 2017 00:04:32 -0600 Authentication-Results: BKDC-DMZ-ESA-02.idc.bsc.rw; dkim=neutral (message not signed) header.i=none Received-SPF: None (BKDC-DMZ-ESA-02.idc.bsc.rw: no sender authenticity information available from domain of laism-at-ascpl.com.my) identity=pra; client-ip=197.243.16.234; receiver=BKDC-DMZ-ESA-02.idc.bsc.rw; envelope-from="laism-at-ascpl.com.my"; x-sender="laism-at-ascpl.com.my"; x-conformance=sidf_compatible Received-SPF: Fail (BKDC-DMZ-ESA-02.idc.bsc.rw: domain of laism-at-ascpl.com.my does not designate 197.243.16.234 as permitted sender) identity=mailfrom; client-ip=197.243.16.234; receiver=BKDC-DMZ-ESA-02.idc.bsc.rw; envelope-from="laism-at-ascpl.com.my"; x-sender="laism-at-ascpl.com.my"; x-conformance=sidf_compatible; x-record-type="v=spf1" Received-SPF: None (BKDC-DMZ-ESA-02.idc.bsc.rw: no sender authenticity information available from domain of postmaster-at-gahinga-whm.idc.bsc.rw) identity=helo; client-ip=197.243.16.234; receiver=BKDC-DMZ-ESA-02.idc.bsc.rw; envelope-from="laism-at-ascpl.com.my"; x-sender="postmaster-at-gahinga-whm.idc.bsc.rw"; x-conformance=sidf_compatible X-IronPort-Anti-Spam-Filtered: true X-IronPort-Anti-Spam-Result: AgEAAMEeUlfF8xDql2dsb2JhbABeHQGCT4IghG+gAxyOLgmEP4RbKIVqAoE0TAEBAQEBARMBAQEBAQgWB08BhD0JAQEBAgEjWwsEBw01AgICVQwBDAiFaoI1BgqxRoYjinYMFw6JcYEDgSIXglkRAYMdEIJJBY5vhm2CaYMtgTuCC4IIgXODLwdogQGET4MJF4VEbIFtgT2BCYozgmgEgVY8MohdgTUBAQE X-IronPort-AV: E=Sophos;i="5.22,559,1449525600"; d="arj'48?exe'48,96?scan'48,96,48,217,208,96";a="115761796" Received: from unknown (HELO gahinga-whm.idc.bsc.rw) ([197.243.16.234]) by BKDC-DMZ-ESA-02.idc.bsc.rw with ESMTP; 14 Nov 2017 08:00:49 +0200 Received: from [127.0.0.1] (port=30537 helo=webmail.rsb.gov.rw) by gahinga-whm.idc.bsc.rw with esmtpa (Exim 4.89) (envelope-from {laism-at-ascpl.com.my} ) id 1eEUF2-00050v-JP; Tue, 14 Nov 2017 03:58:36 -0200 MIME-Version: 1.0 Content-Type: multipart/mixed; boundary="=_310c312b30c961a7db1ba577ca912292"
X-from: marcello.serracino-at-uniroma1.it
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both marcello.serracino-at-uniroma1.it as well as the Microscopy Listserver ---------------------------------------------------------------------------
Message: Dear all, we are in trouble with our SEM FEI Quanta 400, It is not possible to have HV because till the messages listed below appear the hv is halted. we have the following messages: - The gun is in preoperation state and after few seconds - The internal gun communication has been restored.
Every two minutes we have the same alert.
We remove the Wehnelt cylinder, close the gun, repumping, but the problem remain. The vacuum it is ok. Have you any suggestions? Every suggestion will be appreciated
Best regards Marcello
Login Host: 151.100.51.167 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
From ninabuck952u-at-gmail.com Tue Nov 14 23:23:17 2017 Return-Path: {ninabuck952u-at-gmail.com} Received: from gmail.com ([45.115.242.248]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id vAF5NGSN020593 for {microscopylistserverarchive6-at-microscopy.com} ; Tue, 14 Nov 2017 23:23:17 -0600 Received: from unknown (HELO mail.gimmicc.net) (Wed, 15 Nov 2017 14:01:23 +0900) by mail.webhostings4u.com with NNFMP; Wed, 15 Nov 2017 14:01:23 +0900 Received: from mailout.endmonthnow.com [145.101.191.15] by mx03.listsystemsf.net with ASMTP; Wed, 15 Nov 2017 13:42:09 +0900 Received: from unknown (HELO relay.2yahoo.com) (Wed, 15 Nov 2017 13:38:40 +0900) by qrx.quickslick.com with SMTP; Wed, 15 Nov 2017 13:38:40 +0900 Received: from mx.reskind.net ([193.150.9.58]) by qnx.mdrost.com with NNFMP; Wed, 15 Nov 2017 13:25:31 +0900 Message-ID: {02852A22.6A1D1373-at-gmail.com}
Institute of Materials Science, University of Connecticut Position ID: UConn-IMS-2018203 [#10300, 2018203] Position Title: Assistant/Associate/Full Professor, Electron Microscopy Position Type: Tenured/Tenure-track faculty Position Location: Storrs, Connecticut 06269, United States Subject Area: Materials Science / Engineering Appl Deadline: none (posted 2017/10/25) To apply: https://academicjobsonline.org/ajo/jobs/10300
Position Description:
The Institute of Materials Science (IMS) http://www.ims.uconn.edu/ at the University of Connecticut (UConn) seeks qualified candidates for an Electron Microscopy faculty position (Assistant/Associate/Full Professor) experienced with the advanced electron microscopy methods. The IMS consists of faculty members in several different departments of the College of Liberal Arts and Sciences and the School of Engineering. The Department appointment will be based on the expertise of the successful candidate. UConn is in the midst of a transformational period of growth, supported by the $1.7B Next Generation Connecticut (http://nextgenct.uconn.edu/ ), the Tech Park initiative (http://innovation.uconn.edu/tech-park/ ) and a bold new Academic Plan (http://issuu.com/uconnprovost/docs/academic-plan-single-hi-optimized_1 ). We are pleased to continue these investments by inviting applications from eminent scholars who can engage with our IMS faculty members.
DUTIES AND RESPONSIBILITES The successful candidate will be expected to contribute to research and scholarship through extramural funding (in disciplines where applicable), high quality publications, impact as measured through citations, performances, and exhibits (in disciplines where applicable), and national recognition as through honorific awards. In the area of teaching, the successful candidate will share a deep commitment to effective instruction at the undergraduate and graduate levels, development of innovative courses and mentoring of students in research, outreach, and professional development. Successful candidates will also be expected to broaden participation among members of under-represented groups; demonstrate through their research, teaching, and/or public engagement the richness of diversity in the learning experience; integrate multicultural experiences into instructional methods and research tools; and provide leadership in developing pedagogical techniques designed to meet the needs of diverse learning styles and intellectual interests. Successful candidates will teach courses at both the undergraduate and graduate levels, develop internationally recognized, externally-funded research programs, and contribute to the operation and promotion of the Institute, University, and profession through service.
MINIMUM QUALIFICATIONS 1 Applicants must have a Ph.D. in materials science, engineering, physics, chemistry or a related field. 2 Outstanding record of peer-reviewed publications. 3 A history of strong extramurally funded research programs. 4 Experience with teaching courses at the undergraduate and graduate levels. 5 Excellent oral and written communication skills.
PREFERRED QUALIFICATIONS 1 Demonstrated ability to obtain sustained extramural support for research programs. 2 Demonstrated ability to conduct and lead collaborative interdisciplinary research in materials science. 3 Demonstrated ability to work within a research cluster. 4 Excellence in teaching at the undergraduate and graduate levels. 5 Proven commitment to working within a diverse environment.
APPOINTMENT TERMS This is a full-time (9-month) tenure track position within the IMS. The successful candidate’s primary academic appointment will be at the UConn main campus in Storrs, CT, with the possibility of work at UConn’s regional campuses across the state. Salary and rank will be commensurate with qualifications and experience.
TO APPLY Select "Apply Now" to be redirected to Academic Jobs Online to complete your application. Please submit a cover letter; curriculum vitae, including a full list of publications; teaching statement (teaching philosophy, teaching experience, commitment to effective learning, concepts for new course development, etc.); research and scholarship statement (innovative concepts, experience in proposal development, mentorship of post-graduate residents, fellows, and/or graduate students, etc.); and a commitment to diversity statement (including broadening participation, integrating multicultural experiences in instruction and research and pedagogical techniques to meet the needs of diverse learning styles, etc.). Additionally, please follow the instructions on Academic Jobs Online to direct three (3) reference writers to submit letters of reference referencing Electron Microscopy Search # 2018203. Evaluation of applicants will begin immediately and continue until the position is filled. Employment of the successful candidate will be contingent upon the successful completion of a pre-employment criminal background check. (Search # 2018203) All employees are subject to adherence to the State Code of Ethics, which may be found at http://www.ct.gov/ethics/site/default.asp.
The University of Connecticut is committed to building and supporting a multicultural and diverse community of students, faculty, and staff. The diversity of students, faculty, and staff continues to increase, as does the number of honors students, valedictorians and salutatorians who consistently make UConn their top choice. More than 100 research centers and institutes serve the University’s teaching, research, diversity, and outreach missions, leading to UConn’s ranking as one of the nation’s top research universities. UConn’s faculty and staff are the critical link to fostering and expanding our vibrant, multicultural and diverse University community. As an Affirmative Action/Equal Employment Opportunity employer, UConn encourages applications from women, veterans, people with disabilities and members of traditionally underrepresented populations.
Application Materials Required: Submit the following items online at this website to complete your application: • Cover Letter • Curriculum Vitae • Teaching Statement • Research and Scholarship Statement • Commitment to Diversity Statement • Three Reference Letters (to be submitted by the reference writers at https://academicjobsonline.org/ajo/jobs/10300) And anything else requested in the position description.
Further Info: http://www.ims.uconn.edu/
Institute of Materials Science (IMS) 97 North Eagleville Road University of Connecticut Storrs, CT 06269-3136
==============================Original Headers============================== 16, 58 -- From m.aindow-at-uconn.edu Thu Nov 16 12:53:44 2017 16, 58 -- Received: from NAM03-BY2-obe.outbound.protection.outlook.com (mail-by2nam03on0095.outbound.protection.outlook.com [104.47.42.95]) 16, 58 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id vAGIriaD031424 16, 58 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Nov 2017 12:53:44 -0600 16, 58 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=uconn.onmicrosoft.com; 16, 58 -- s=selector1-uconn-edu; 16, 58 -- h=From:Date:Subject:Message-ID:Content-Type:MIME-Version; 16, 58 -- bh=nqc5k0Y9BooqZvPWBc1vTw2/Srng+jZ5Z0qzl3A21XI=; 16, 58 -- b=NZ5gpQGEQsPrmnwXwv2JlZF/zFnu1g1ltESXQ2xJqM/Xxa2gKlWKK2EfwzS4xATe9reeso+KoFAyanRxCHJNeHXDP7dUNNh35RJKC9IDu1bUt3GiWwH8RaIF6dQBOD0VouKXTMDTEfzkkQtvIWD+K8tB5uJonXURpJPchMZZJYs= 16, 58 -- Received: from CY1PR05MB2730.namprd05.prod.outlook.com (10.167.18.12) by 16, 58 -- CY1PR05MB2729.namprd05.prod.outlook.com (10.167.18.11) with Microsoft SMTP 16, 58 -- Server (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_256_CBC_SHA384_P384) id 16, 58 -- 15.20.239.4; Thu, 16 Nov 2017 18:50:13 +0000 16, 58 -- Received: from CY1PR05MB2730.namprd05.prod.outlook.com ([10.167.18.12]) by 16, 58 -- CY1PR05MB2730.namprd05.prod.outlook.com ([10.167.18.12]) with mapi id 16, 58 -- 15.20.0239.005; Thu, 16 Nov 2017 18:50:13 +0000 16, 58 -- From: "Aindow, Mark" {m.aindow-at-uconn.edu} 16, 58 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 16, 58 -- Subject: Faculty Position in Electron Microscopy 16, 58 -- Thread-Topic: Faculty Position in Electron Microscopy 16, 58 -- Thread-Index: AQHTXwu8O85oIDWgjUafUbrcghnjhw== 16, 58 -- Date: Thu, 16 Nov 2017 18:50:13 +0000 16, 58 -- Message-ID: {96E0028C-16D7-43AB-9B79-2177CCD6200A-at-uconn.edu} 16, 58 -- Accept-Language: en-US 16, 58 -- Content-Language: en-US 16, 58 -- X-MS-Has-Attach: 16, 58 -- X-MS-TNEF-Correlator: 16, 58 -- user-agent: Microsoft-MacOutlook/f.28.0.171108 16, 58 -- authentication-results: spf=none (sender IP is ) 16, 58 -- smtp.mailfrom=m.aindow-at-uconn.edu; 16, 58 -- x-originating-ip: [137.99.20.89] 16, 58 -- x-ms-publictraffictype: Email 16, 58 -- x-microsoft-exchange-diagnostics: 1;CY1PR05MB2729;6:xEDvQ+qgY3lM8At4I87l/1l3pGGX+VSiJWyR3vOjIy8dXsSXvFBfw6zcL+qlBlh8z/a3XnN/eR8yxeYuekVnNJEISef+LUeecsOpWfCs0CRni9gPAvWwOHck+cbbUmNsLIl7stOpQSRuOZdszwqpIhsAmbYcxVyQLbcUYyK54Y9GEQh4zFJdm6Q0OKDqHtihVnH4a18k1HzeYYItwCtHurdz1sLt4iQDCS2sj40ItZllkxtGTxb+YMFPyuJCegOUr+/MgndB4A6gIhG7b0rOk2OMnnZ7dEM1rgC/NDnNrzy7/y/CKCMliH7tqdUh4whok926NiIEifuRsvrjATB12LkdmGxu38q96G+yViAs6m8=;5:4nsnQodSotouqhIKCsGh+2Q6nCGWC7NBxZtwLiiJOYl3AD/zg+qhouIyNLHhM85PqZ6IU2dkfrqSIvY867kO8U56xTyNOpdoW6IPpDuLhhXBnl+V+Fd5RNEPJEP2mHUrMBZDfk6R6DshKGvVCRqJpAEKJ8Uln92ztai+YbI6Yl4=;24:So9tTLpP6/zRJvdDF62+nYS8y81yCavaxd9paD79d0d4hU37ZLifxfEzxeNjUhCAok2VaYQjHP62YQBFQxcP8H7Al+7vo/WjC2bgjOAsdnE=;7:fAgIzFGyUKqusFmk/MDETTYjmKPlxdha0oVkwxU8MGyB21q6Yaiqr2luHDeEWzuCssO58gJaQgatVUiDDpIvHCVkzmVLt1Q7CulSCo9cZDuCnyHuxzQRcoxsMG0gZI4lTKNiqz65GApTSsM8peJhaJxDNVK9PAASbyQ203Cz3xpkvyPw/G3UJ0dNaW33HN1iW0XlNCwU6Oy95Q9R7t1Id5DCk/CdpBboN0BtuHpC6fKjRBeL9BVB/s36mJEjQ4F3 16, 58 -- x-ms-exchange-antispam-srfa-diagnostics: SSOS; 16, 58 -- x-ms-office365-filtering-correlation-id: 31f1fa33-c005-4400-c5de-08d52d22df02 16, 58 -- x-microsoft-antispam: UriScan:;BCL:0;PCL:0;RULEID:(22001)(4534020)(4602075)(4627115)(201703031133081)(201702281549075)(2017052603199);SRVR:CY1PR05MB2729; 16, 58 -- x-ms-traffictypediagnostic: CY1PR05MB2729: 16, 58 -- x-microsoft-antispam-prvs: {CY1PR05MB2729EC152D3C3DF8B16A662B802E0-at-CY1PR05MB2729.namprd05.prod.outlook.com} 16, 58 -- x-exchange-antispam-report-test: UriScan:(99511279444120)(278428928389397)(209352067349851)(144335513081603)(82855064376076); 16, 58 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:(100000700101)(100105000095)(100000701101)(100105300095)(100000702101)(100105100095)(6040450)(2401047)(5005006)(8121501046)(93006095)(93001095)(100000703101)(100105400095)(10201501046)(3231022)(3002001)(6041248)(20161123555025)(20161123562025)(20161123560025)(20161123564025)(20161123558100)(201703131423075)(201702281529075)(201702281528075)(201703061421075)(201703061406153)(6072148)(201708071742011)(100000704101)(100105200095)(100000705101)(100105500095);SRVR:CY1PR05MB2729;BCL:0;PCL:0;RULEID:(100000800101)(100110000095)(100000801101)(100110300095)(100000802101)(100110100095)(100000803101)(100110400095)(100000804101)(100110200095)(100000805101)(100110500095);SRVR:CY1PR05MB2729; 16, 58 -- x-forefront-prvs: 0493852DA9 16, 58 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10019020)(6009001)(346002)(376002)(189002)(199003)(50944005)(106356001)(25786009)(105586002)(33656002)(66066001)(3660700001)(97736004)(6486002)(2351001)(99286004)(81156014)(81166006)(4743002)(101416001)(561944003)(8676002)(6506006)(5640700003)(7736002)(88552002)(77096006)(3280700002)(2906002)(6916009)(478600001)(5660300001)(2501003)(86362001)(6116002)(6436002)(316002)(966005)(305945005)(36756003)(50986999)(14454004)(75432002)(58126008)(53376002)(54356999)(1720100001)(83506002)(2900100001)(82746002)(6512007)(53936002)(189998001)(3846002)(68736007)(786003)(102836003)(6306002)(8936002)(83716003);DIR:OUT;SFP:1102;SCL:1;SRVR:CY1PR05MB2729;H:CY1PR05MB2730.namprd05.prod.outlook.com;FPR:;SPF:None;PTR:InfoNoRecords;MX:1;A:1;LANG:en; 16, 58 -- received-spf: None (protection.outlook.com: uconn.edu does not designate 16, 58 -- permitted sender hosts) 16, 58 -- spamdiagnosticoutput: 1:99 16, 58 -- spamdiagnosticmetadata: NSPM 16, 58 -- Content-Type: text/plain; charset="utf-8" 16, 58 -- Content-ID: {02CE428DDCF3B045BC35165B23976280-at-namprd05.prod.outlook.com} 16, 58 -- MIME-Version: 1.0 16, 58 -- X-OriginatorOrg: uconn.edu 16, 58 -- X-MS-Exchange-CrossTenant-Network-Message-Id: 31f1fa33-c005-4400-c5de-08d52d22df02 16, 58 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 16 Nov 2017 18:50:13.4088 16, 58 -- (UTC) 16, 58 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted 16, 58 -- X-MS-Exchange-CrossTenant-id: 17f1a87e-2a25-4eaa-b9df-9d439034b080 16, 58 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: CY1PR05MB2729 16, 58 -- Content-Transfer-Encoding: 8bit 16, 58 -- X-MIME-Autoconverted: from base64 to 8bit by microscopy.com id vAGIriaD031424 ==============================End of - Headers==============================
From ralpzand8seine-at-gmail.com Thu Nov 16 17:45:32 2017 Return-Path: {ralpzand8seine-at-gmail.com} Received: from gmail.com ([45.115.242.244]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id vAGNjV8o027438 for {microscopylistserverarchive6-at-microscopy.com} ; Thu, 16 Nov 2017 17:45:32 -0600 Received: from smtp.endend.nl ([210.133.154.25]) by external.newsubdomain.com with SMTP; Thu, 16 Nov 2017 14:25:15 -0900 Received: from snmp.otwaloow.com ([Thu, 16 Nov 2017 14:10:17 -0900]) by asx121.turbo-inline.com with SMTP; Thu, 16 Nov 2017 14:10:17 -0900 Received: from mx.reskind.net [27.203.178.164] by asx121.turbo-inline.com with SMTP; Thu, 16 Nov 2017 14:03:52 -0900 Received: from smtp4.cyberemailings.com ([67.202.109.179]) by relay37.vosimerkam.net with LOCAL; Thu, 16 Nov 2017 13:52:02 -0900 Received: from unknown (19.53.65.177) by mmx09.tilkbans.com with ESMTP; Thu, 16 Nov 2017 13:41:03 -0900 Message-ID: {43AA4063.36E18423-at-gmail.com}
Dear List Our SEM JSM5600LV burns filaments immediately upon turning high tension ON. There was a thunderstorm here and after a big thunder the microscope went completely OFF while there was no power cut. Very soon the microscopes power came back and worked normally but I noticed the image was bit noisy and definition less high. Then I switched it off overnight and today I started and realized the problem (after I burned a couple of filaments). Any comment or suggestion will be greatly appreciated
Dear colleagues - we are opening postdoctoral position(s) in the field of direct atom-by-atom fabrication by sub-atomically focused beam of Scanning Transmission Electron Microscope. The recent examples of the work the candidate will be involved in are available in recent publications (arXiv:1711.05810 {https://arxiv.org/abs/1711.05810} , arXiv:1710.10338 {https://arxiv.org/abs/1710.10338} , arXiv:1710.09416 {https://arxiv.org/abs/1710.09416} , arXiv:1708.01523 {https://arxiv.org/abs/1708.01523} , arXiv:1709.00470 {https://arxiv.org/abs/1709.00470} ). The ideal candidate will have expertise in atomically resolved STEM, image simulation, and Python programming. Please contact Sergei Kalinin and Stephen Jesse directly (sergei2-at-ornl.gov and sjz-at-ornl.gov) if interested. Sergei
-- Sergei V. Kalinin
Director, Institute for Functional Imaging of Materials
Fellow MRS, APS, AVS
Oak Ridge National Laboratory
Phone: (865) 241-0236
==============================Original Headers============================== 6, 37 -- From sergei2-at-ornl.gov Fri Nov 17 09:02:33 2017 6, 37 -- Received: from mta02.ornl.gov (mta02.ornl.gov [128.219.177.136]) 6, 37 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id vAHF2XV5022421 6, 37 -- for {microscopy-at-microscopy.com} ; Fri, 17 Nov 2017 09:02:33 -0600 6, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 37 -- d=ornl.gov; i=-at-ornl.gov; q=dns/txt; s=p20151116; 6, 37 -- t=1510930747; x=1542466747; 6, 37 -- h=to:from:subject:message-id:date:mime-version: 6, 37 -- content-transfer-encoding; 6, 37 -- bh=/AeYleAAJ16CJPwVTfp2aC6k3yZHPMcJdJscEI8AC24=; 6, 37 -- b=TOZeP8lEJWHTYZnqYJZJudAuaHRY6wsaEqUiB0uxc/j7oPVXTaolIcpp 6, 37 -- noJnCd3psu1j2S1DANh76/UdShlerf+KlL7IbQIiXXi9xnfhXR58fbe5m 6, 37 -- HN6miJMnsYx1eJDha49oQtyKtoQFUu8XeRBLFB132u6q6YT4R8Fhk9OCX 6, 37 -- zTo8Ekyn7dvcdh3pubFKuKT6lyEk9PKpn4An1x0AJHD0LPwRZ2beJooNh 6, 37 -- LGiYf+7lVvitmmLzUQymT6643m2jJ5D0FNkUdA+MiGoxS/5yxAihFIog8 6, 37 -- zyE9AsprFgOnfSUUBE5hR+ygMZjtAeAKdOtmYQtB8UZAbmh3aYV1mRruM 6, 37 -- A==; 6, 37 -- X-SG: RELAYLIST 6, 37 -- X-IronPort-AV: E=Sophos;i="5.44,410,1505793600"; 6, 37 -- d="scan'208";a="26139111" 6, 37 -- Received: from emgwy1.ornl.gov ([160.91.254.9]) 6, 37 -- by iron2.ornl.gov with ESMTP/TLS/DHE-RSA-AES256-GCM-SHA384; 17 Nov 2017 09:59:06 -0500 6, 37 -- Received: from [128.219.192.116] (pc95228.ornl.gov [128.219.192.116]) 6, 37 -- (using TLSv1.2 with cipher ECDHE-RSA-AES128-GCM-SHA256 (128/128 bits)) 6, 37 -- (No client certificate requested) 6, 37 -- by emgwy1.ornl.gov (Postfix) with ESMTPS id 3ydh8V3FVmz2T6bp 6, 37 -- for {microscopy-at-microscopy.com} ; Fri, 17 Nov 2017 09:59:06 -0500 (EST) 6, 37 -- To: microscopy-at-microscopy.com 6, 37 -- From: "Sergei V. Kalinin" {sergei2-at-ornl.gov} 6, 37 -- Subject: Postdoctoral position: Atom-by-atom fabrication by e-beam in STEM 6, 37 -- Message-ID: {5A0EF93A.6070404-at-ornl.gov} 6, 37 -- Date: Fri, 17 Nov 2017 09:59:06 -0500 6, 37 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:38.0) Gecko/20100101 6, 37 -- Thunderbird/38.0.1 6, 37 -- MIME-Version: 1.0 6, 37 -- Content-Type: text/plain; charset=utf-8; format=flowed 6, 37 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both fay1-at-email.arizona.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] SEM-CL missing red wavelengths?
Message: Dear Microscopy Community:
Does anybody know why an SEM-CL instrument could be missing the red-to-orange wavelengths of the cathodoluminescence spectrum?
I run a JEOL 6010LA benchtop SEM wit a recently acquired Gatan MonoCL4 cathodoluminescence spectrometer. It works great for zircons, quartz, etc., but picks up little to no signal in the red to orange part of the spectrum even on samples that glow brilliant red under our ancient optical cold-cathode Luminoscope. I checked the same samples out on a ChromaCL hooked up to a Hitachi S3400 in another lab, and there is a similar lack of red signal. I've checked and the accelerating voltages on the Luminoscope are about the same as on the SEM, although the Luminoscope has higher beam current, so I'm not sure why the SEM-CL instruments don't pick up the red luminescence. Ive read that filters placed in front of a detector can eliminate red-orange luminescence and phosphorescence (Reed and Milliken, Journal of Sedimentary Research 73.2: 328-332). There was no mention of such a filter in the Gatan datasheets for either the ChromaCL or the MonoCL4, but that seems to me to be one possible explanation for the lack of red luminescence in SEM-CL compared to optical cold-cathode CL. I've left a message with Gatan but haven't heard back yet. Has anybody else with SEM-CL encountered a similar problem, or know of any fixes? We got the MonoCL4 partly in order to look at carbonate cements in rocks, so its pretty important that we figure out how to get good spectra and images in the red and orange zones.
Any help would be appreciated. Thanks,
Isabel Barton
Login Host: 68.106.21.127 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both annie.muske-dukes-driggs-at-lonza.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] EDS and SEM projector for Outreach
Message: Hi everyone,
My company runs a community outreach program for local elementary school students (8-10 years old). They tour our facility and see quick demonstrations/lectures on a variety of topics. We have had an SEM demo for years and I would like to incorporate our EDS but I'm having trouble coming up with samples that kids that age understand, especially since I only have 15 minutes per group and we try to go through at least four samples.
I have generated a map of different elements in a vitamin tablet which went over well and gives them a good understanding of what the system is doing. Any recommendations for samples that generate interesting maps or have unexpected elements? I'll be doing the prep/high quality maps ahead of time so that doesn't need to be done in the 15 minutes.
I'd also like to get a wireless projector to use with our Hitachi SU3500 to project a live image of the screen onto the wall. Our service rep said some models can cause driver issues so if anyone here has a model that works please let me know. We need to stay under US$500, but under $300 would be ideal.
Thanks, Annie (I am a list subscriber but our required corporate attachments prevent me from sending the usual way)
Login Host: 64.47.109.63 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
X-from: walbrid-at-cshl.edu This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both walbrid-at-cshl.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: walbrid-at-cshl.edu Name: Samantha
Organization: Cold Spring Harbor Laboratory
Title-Subject: [Filtered] Electron Microscopy Position Available
Message: Hello,
Cold Spring Harbor Laboratory is currently seeking an electron microscopy technician. Please see below for details.
Research Associate Electron Microscopy Technologist
Cold Spring Harbor Laboratory seeks a highly motivated dedicated individual to work in a state-of-the-art Microscopy Shared Resource.
The individual should have extensive practical expertise in biological sample preparation for transmission and scanning electron microscopy. Hands-on knowledge of confocal and widefield fluorescence microscopy would also be a plus. The candidate will help users design innovative experiments and they will carry out sample preparation and imaging as well as assist in data interpretation.
Excellent verbal and written communication skills, ability to work with multiple users in a supporting role, and ability to work independently and proactively with limited supervision are essential. A Bachelors degree in biology or related discipline is required. One to three years of experience working in a Microscopy Shared Resource is preferred.
How to Apply
Interested individuals should apply for this position via the CSHL careers website at https://www.cshl.edu/careers.
Position Number 01779-R
Applicants should include a resume along with a description of their practical expertise and the names as well as email addresses of 3 references.
Cold Spring Harbor Laboratory is a world-renowned research and educational institution recognized internationally for its excellence in ground-breaking research programs in cancer, neuroscience, plant biology, genomics, and bioinformatics and broad educational mission.
For more information about CSHL, please visit us at www.cshl.edu
CSHL is an EO/AA Employer. All qualified applicants will receive consideration for employment and will not be discriminated against on the basis of race, color, religion, sex, sexual orientation, gender identity, national origin, age, disability or protected veteran status. VEVRAA Federal Contractor
Login Host: 143.48.14.89 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mholman-at-mvainc.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: mholman-at-mvainc.com Name: Melissa Holman
Organization: MVA Scientific Consultants
Title-Subject: [Filtered] Job Opening - microscopist with Raman & IR experience
Message: MVA Scientific Consultants, a premier microscopy and microanalysis laboratory and consulting group located in Atlanta, GA, is looking for a microscopist with experience in the preparation and analysis of microscopic samples by confocal Raman microscopy and infrared microspectroscopy with an emphasis on materials and pharmaceutical samples. The ideal candidate will have a minimum of a Bachelors degree in a science field and several years of experience.
Please contact Melissa Holman at mholman-at-mvainc.com for more information.
Login Host: 47.44.47.92 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
I’m retiring from the University of Washington and would like to thank the members of this listserv for the informative discussions and helpful answers to my questions over the many years that I’ve been subscribed. An especially big thanks to Nestor for getting it started and keeping it running for so long.
My email address will remain active. But, any inquiries regarding the Digital Microscopy Center should be directed to my replacement, Kim Miller, kimiline-at-uw.edu; especially if you are interested in using the resources :-)
Regards, Glen Glen MacDonald Digital Microscopy Center Box 357923 University of Washington Seattle, WA 98195-7923 USA (206) 616-4156 depts.washington.edu/digmicro glenmac-at-uw.edu
I’ve had good results showing young people fossils, shells or dinosaur bone fragments. Kids love those! Also, teeth, and hair can be interesting. Hair is especially captivating because they can donate it from their own head and it becomes personal. It also contains S which is a surprise to most people.
Cheers,
Zack
} On Nov 17, 2017, at 3:39 PM, microscopy.listserver-at-gmail.com wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: annie.muske-dukes-driggs-at-lonza.com } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both annie.muske-dukes-driggs-at-lonza.com as well } as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: annie.muske-dukes-driggs-at-lonza.com Name: Annie Muske-Dukes } } Organization: Bend Research } } Title-Subject: [Filtered] EDS and SEM projector for Outreach } } Message: Hi everyone, } } My company runs a community outreach program for local elementary school } students (8-10 years old). They tour our facility and see quick } demonstrations/lectures on a variety of topics. } We have had an SEM demo for years and I would like to incorporate our } EDS but I'm having trouble coming up with samples that kids that age } understand, especially since I only have 15 minutes per group and we try } to go through at least four samples. } } I have generated a map of different elements in a vitamin tablet which } went over well and gives them a good understanding of what the system is } doing. Any recommendations for samples that generate interesting maps or } have unexpected elements? I'll be doing the prep/high quality maps ahead } of time so that doesn't need to be done in the 15 minutes. } } I'd also like to get a wireless projector to use with our Hitachi SU3500 } to project a live image of the screen onto the wall. Our service rep } said some models can cause driver issues so if anyone here has a model } that works please let me know. We need to stay under US$500, but under } $300 would be ideal. } } Thanks, } Annie (I am a list subscriber but our required corporate attachments } prevent me from sending the usual way) } } Login Host: 64.47.109.63 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 13, 53 -- From microscopy.listserver-at-gmail.com Fri Nov 17 17:18:56 2017 } 13, 53 -- Received: from mail-pf0-f175.google.com (mail-pf0-f175.google.com [209.85.192.175]) } 13, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id vAHNItoN009038 } 13, 53 -- for {microscopy-at-microscopy.com} ; Fri, 17 Nov 2017 17:18:55 -0600 } 13, 53 -- Received: by mail-pf0-f175.google.com with SMTP id x7so2994822pfa.1 } 13, 53 -- for {microscopy-at-microscopy.com} ; Fri, 17 Nov 2017 15:15:30 -0800 (PST) } 13, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 13, 53 -- d=gmail.com; s=20161025; } 13, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 13, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 13, 53 -- bh=ajHXaowS7Xr4ulq5L7g6cSgXpHzLsIrp0ixP0W269Yg=; } 13, 53 -- b=eAW4Xc2CZ5KfsTKhl5W7M/p2Y/WXg6iJXwJQo9hPbmsciy3Vck/Ax2o7BirKvnpdNX } 13, 53 -- OHTQaVaboEyxaXc7fXVUBMmkkmN+iV6G180P48hIK3TBBvUpZT2G4LwsRl/uSiTcn+nz } 13, 53 -- x5fEA9ygmixMEaa6gk6e/kYBbOHHJY91XyIfC5mpUS8mYQd9W03hYSd9j4792grF6hYh } 13, 53 -- Y6iq/B2scCSA1R0hxix7wW4L+fJUpFRTNthPfi3uuMdf9+Nmi1yAEC+3Pi9kr69xnL4U } 13, 53 -- 8rmapnMno5eW0z7bA3tUYDUxmCzw9TSppsLLY02yiL+Qzvt+7X6TZQGvW3MeDZynUCL4 } 13, 53 -- Jd5Q== } 13, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 13, 53 -- d=1e100.net; s=20161025; } 13, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 13, 53 -- :user-agent:mime-version:in-reply-to:content-language } 13, 53 -- :content-transfer-encoding; } 13, 53 -- bh=ajHXaowS7Xr4ulq5L7g6cSgXpHzLsIrp0ixP0W269Yg=; } 13, 53 -- b=UE5U+qloOyYI02cv3X62Aen7ygRVtpC9be1euW6ToL9RWOUNDzJuYicaiYHteGM+Qd } 13, 53 -- qtJ39xxJ5fCRtWOA/2craX4llZDjvyEvBu8qvgFEu0rF1s6Iq6mVP22f81Ue/sr9d/IR } 13, 53 -- 6fP5utPLZ77wf8zE03W8OF7aAh6jcs2CqcLdDOKut29d29W/tXo2uc/6fvFGZfrm0KC4 } 13, 53 -- Y8escGfPPpC2PjflO0YYjziQs6DRvEKHz2P6szOnnILH98LcBZW5PuiKEXBwpNSTgFMj } 13, 53 -- Fq8fQCSelKePgB2xQ4HTqJJWfkTSxsRX+YTD+e8VukuWKoI90w/IYGW2+8/ep8UuppN7 } 13, 53 -- bMQA== } 13, 53 -- X-Gm-Message-State: AJaThX6HbDIzxLHVVc7CeFhyQ7NEofsBNhRwjrnchA1onNOMwoQ/xU4k } 13, 53 -- GlNT9VefdH3phrwGEcxoF+YUtwv8 } 13, 53 -- X-Google-Smtp-Source: AGs4zMa17Mah5ciiqmYbjFsgBJIMp4Q9FQSWDIx5LCnxz99Kp3+HdN2/YzJUDJMfrLF91MumAUhpXQ== } 13, 53 -- X-Received: by 10.84.193.129 with SMTP id f1mr6807886pld.317.1510960529479; } 13, 53 -- Fri, 17 Nov 2017 15:15:29 -0800 (PST) } 13, 53 -- Received: from Nestors-MacBookAir-Pro-2014-ElCapitan-OSX-1163.local ([182.16.234.14]) } 13, 53 -- by smtp.googlemail.com with ESMTPSA id p19sm9352707pfj.140.2017.11.17.15.15.24 } 13, 53 -- for {microscopy-at-microscopy.com} } 13, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 13, 53 -- Fri, 17 Nov 2017 15:15:24 -0800 (PST) } 13, 53 -- Subject: viaWWW:EDS and SEM projector for Outreach } 13, 53 -- References: {201711162229.vAGMTWp6026431-at-microscopy.com} } 13, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 13, 53 -- From: Microscopy Listserver {microscopy.listserver-at-gmail.com} } 13, 53 -- X-Forwarded-Message-Id: {201711162229.vAGMTWp6026431-at-microscopy.com} } 13, 53 -- Message-ID: {4230175d-0ecf-bad3-c4f0-4f12f99a8f95-at-gmail.com} } 13, 53 -- Date: Sat, 18 Nov 2017 07:15:22 +0800 } 13, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) } 13, 53 -- Gecko/20100101 Thunderbird/52.4.0 } 13, 53 -- MIME-Version: 1.0 } 13, 53 -- In-Reply-To: {201711162229.vAGMTWp6026431-at-microscopy.com} } 13, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 13, 53 -- Content-Language: en-US } 13, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
Our EDS is showing a high intensify peak at about 0.2 keV even with beam on vacuum. We're not getting C signal anymore and the energy resolution is getting worse. There'is been an energy shift also. The issue remains after run an automatic callibraion. Does anybody know what more could be done? Or is it a sign that our detector is almost dead?
Regards,
Erico Freitas Físico/Physicist Centro de Microscopia Universidade Federal de Minas Gerais
Coordenador do Lab. de Microscopia Eletrônica dr Transmissão
Dear colleagues, Does anyone have experience with companies such as LBR Scientific, Cryostar, or other 3rd party providers of service contracts for lab equipment (fume hoods, autoclaves, cold rooms, etc)?
I would appreciate any insight you can share, both positive and negative. Stefanie
********************* Dr. Stefanie Brachfeld Acting Associate Dean for Academic Affairs, College of Science and Mathematics Montclair State University Montclair, NJ 07043 phone: (973) 655-5129 brachfelds-at-mail.montclair.edu *********************
==============================Original Headers============================== 3, 39 -- From brachfelds-at-montclair.edu Sun Nov 19 11:33:08 2017 3, 39 -- Received: from mail-oi0-f51.google.com (mail-oi0-f51.google.com [209.85.218.51]) 3, 39 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id vAJHX73p000549 3, 39 -- for {Microscopy-at-microscopy.com} ; Sun, 19 Nov 2017 11:33:07 -0600 3, 39 -- Received: by mail-oi0-f51.google.com with SMTP id y206so4780210oiy.4 3, 39 -- for {Microscopy-at-microscopy.com} ; Sun, 19 Nov 2017 09:29:47 -0800 (PST) 3, 39 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 39 -- d=mail-montclair-edu.20150623.gappssmtp.com; s=20150623; 3, 39 -- h=mime-version:from:date:message-id:subject:to; 3, 39 -- bh=QZJEQvCw0tMI44k3wYfI8DaEHy+jIDEF36H1XOnnuEY=; 3, 39 -- b=PvqxI0qqRWLpXx7ULKjUMnjyefcP00Z/v8hSBLk6GGte1W0MGmicwTCZqbys/2h8Up 3, 39 -- ZEArOX7Ri/oirb0q1yK830BCWI/9Pg+J5gp+96XkFRlHJErKcNtKMNZ+Lc3UArKrSnP0 3, 39 -- QllyWMfFp7hJdVf3k2VyLztbS0z23N4urWUNmoeJGMtgITS8y6aAv1xRCxLaV3opsluS 3, 39 -- ychhyHPRCirqGZWdF9LT/pbgqw3veMFzP9P8KsSWFOdv2rq9pqL2ZI3rZzyQ9ZVqXJNO 3, 39 -- L+fJHR0ZabhecM8TYxwvzXAymMImz7I6mujHjoOcfC+C9FHk1kIaesm8GAzskMiRAyfQ 3, 39 -- Lwfg== 3, 39 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 39 -- d=1e100.net; s=20161025; 3, 39 -- h=x-gm-message-state:mime-version:from:date:message-id:subject:to; 3, 39 -- bh=QZJEQvCw0tMI44k3wYfI8DaEHy+jIDEF36H1XOnnuEY=; 3, 39 -- b=eOBtaeqk2lIaX1KnbsHDWtD7hKZNCvOcKFUZ9cFQ9eymZleT/25l00QKpI0ymNU/SL 3, 39 -- HuD0DrhYKIuAtXJkNiHFQ0+ej/zU2smWyv23+TNWfS6KYY3+fBK6McPiYxZ/8Iag/jR4 3, 39 -- z6Sn2FzvHoxOuBVVW78VHZ7D+m8ySmQIvrMrUKJidOwB1/NvMNOYDCTc22nKGGmUx5l/ 3, 39 -- IDKPNdCoEj1/DcUzhjCu27R5cODXvKmQpKlZt1Z67dufNOmJXUhYqaMz/7Ba17no3U5n 3, 39 -- lppwhKME0l8CaA/FGsZQuh1tjTI4UJ92T9GZcMfTZGm2G6BEygdANA930L94EWcmwJiS 3, 39 -- WvDg== 3, 39 -- X-Gm-Message-State: AJaThX5s58vgJAe9TLrFCT3ioEIwWSnmsHwiVn/kK5nXpv1rj3p7ioi3 3, 39 -- rgtLdy6mrZaO8NwdoyqZiNb84a3egTH6mORjMaXtYAop 3, 39 -- X-Google-Smtp-Source: AGs4zMZNcHUnKq920SkuXt6IuCpmY2UqWIEtc2sv9VHAI5dSnlgi4Y0aLhccm1Y/rPd5UQwRAMrokpr3oi8QN9aCgsA= 3, 39 -- X-Received: by 10.202.171.196 with SMTP id u187mr5101253oie.255.1511112587190; 3, 39 -- Sun, 19 Nov 2017 09:29:47 -0800 (PST) 3, 39 -- MIME-Version: 1.0 3, 39 -- Received: by 10.74.176.138 with HTTP; Sun, 19 Nov 2017 09:29:06 -0800 (PST) 3, 39 -- From: Stefanie Brachfeld {brachfelds-at-mail.montclair.edu} 3, 39 -- Date: Sun, 19 Nov 2017 12:29:06 -0500 3, 39 -- Message-ID: {CACysd-01Gy1wO6BriMEtxzoBLg4mVbEyscftHfhYbo9ThBR52Q-at-mail.gmail.com} 3, 39 -- Subject: Service contract providers for laboratory equipment 3, 39 -- To: Microscopy-at-microscopy.com 3, 39 -- Content-Type: text/plain; charset="UTF-8" ==============================End of - Headers==============================
X-from: jacques faerber {jacques.faerber-at-ipcms.unistra.fr}
Hi
I'got once a nice paper towel with Christmas decorations, gold, silver and black patterns on the blue towel. It is particulary stiff and looks very close to a real woven towel. So I kept one for SEM practicals with students. You schould be able to find something similar. I didn't try it with joung chidren but I imagine it could do the job. They can have the tovel in their hands, and see in Se/BSe a synthetic/cellulose fiber network, in BSe a compositionnal contraste image and by EDS in spectrum mode schowing that the "gold" is brass, the "silver" is aluminum and the black is carbon... All what shines is not gold...
Best regards Jacques
Le 18/11/2017 à 00:49, microscopy.listserver-at-gmail.com a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: annie.muske-dukes-driggs-at-lonza.com } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both annie.muske-dukes-driggs-at-lonza.com as well } as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: annie.muske-dukes-driggs-at-lonza.com Name: Annie Muske-Dukes } } Organization: Bend Research } } Title-Subject: [Filtered] EDS and SEM projector for Outreach } } Message: Hi everyone, } } My company runs a community outreach program for local elementary school } students (8-10 years old). They tour our facility and see quick } demonstrations/lectures on a variety of topics. } We have had an SEM demo for years and I would like to incorporate our } EDS but I'm having trouble coming up with samples that kids that age } understand, especially since I only have 15 minutes per group and we try } to go through at least four samples. } } I have generated a map of different elements in a vitamin tablet which } went over well and gives them a good understanding of what the system is } doing. Any recommendations for samples that generate interesting maps or } have unexpected elements? I'll be doing the prep/high quality maps ahead } of time so that doesn't need to be done in the 15 minutes. } } I'd also like to get a wireless projector to use with our Hitachi SU3500 } to project a live image of the screen onto the wall. Our service rep } said some models can cause driver issues so if anyone here has a model } that works please let me know. We need to stay under US$500, but under } $300 would be ideal. } } Thanks, } Annie (I am a list subscriber but our required corporate attachments } prevent me from sending the usual way) } } Login Host: 64.47.109.63 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 13, 53 -- From microscopy.listserver-at-gmail.com Fri Nov 17 17:18:56 2017 } 13, 53 -- Received: from mail-pf0-f175.google.com (mail-pf0-f175.google.com [209.85.192.175]) } 13, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id vAHNItoN009038 } 13, 53 -- for {microscopy-at-microscopy.com} ; Fri, 17 Nov 2017 17:18:55 -0600 } 13, 53 -- Received: by mail-pf0-f175.google.com with SMTP id x7so2994822pfa.1 } 13, 53 -- for {microscopy-at-microscopy.com} ; Fri, 17 Nov 2017 15:15:30 -0800 (PST) } 13, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 13, 53 -- d=gmail.com; s=20161025; } 13, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 13, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 13, 53 -- bh=ajHXaowS7Xr4ulq5L7g6cSgXpHzLsIrp0ixP0W269Yg=; } 13, 53 -- b=eAW4Xc2CZ5KfsTKhl5W7M/p2Y/WXg6iJXwJQo9hPbmsciy3Vck/Ax2o7BirKvnpdNX } 13, 53 -- OHTQaVaboEyxaXc7fXVUBMmkkmN+iV6G180P48hIK3TBBvUpZT2G4LwsRl/uSiTcn+nz } 13, 53 -- x5fEA9ygmixMEaa6gk6e/kYBbOHHJY91XyIfC5mpUS8mYQd9W03hYSd9j4792grF6hYh } 13, 53 -- Y6iq/B2scCSA1R0hxix7wW4L+fJUpFRTNthPfi3uuMdf9+Nmi1yAEC+3Pi9kr69xnL4U } 13, 53 -- 8rmapnMno5eW0z7bA3tUYDUxmCzw9TSppsLLY02yiL+Qzvt+7X6TZQGvW3MeDZynUCL4 } 13, 53 -- Jd5Q== } 13, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 13, 53 -- d=1e100.net; s=20161025; } 13, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 13, 53 -- :user-agent:mime-version:in-reply-to:content-language } 13, 53 -- :content-transfer-encoding; } 13, 53 -- bh=ajHXaowS7Xr4ulq5L7g6cSgXpHzLsIrp0ixP0W269Yg=; } 13, 53 -- b=UE5U+qloOyYI02cv3X62Aen7ygRVtpC9be1euW6ToL9RWOUNDzJuYicaiYHteGM+Qd } 13, 53 -- qtJ39xxJ5fCRtWOA/2craX4llZDjvyEvBu8qvgFEu0rF1s6Iq6mVP22f81Ue/sr9d/IR } 13, 53 -- 6fP5utPLZ77wf8zE03W8OF7aAh6jcs2CqcLdDOKut29d29W/tXo2uc/6fvFGZfrm0KC4 } 13, 53 -- Y8escGfPPpC2PjflO0YYjziQs6DRvEKHz2P6szOnnILH98LcBZW5PuiKEXBwpNSTgFMj } 13, 53 -- Fq8fQCSelKePgB2xQ4HTqJJWfkTSxsRX+YTD+e8VukuWKoI90w/IYGW2+8/ep8UuppN7 } 13, 53 -- bMQA== } 13, 53 -- X-Gm-Message-State: AJaThX6HbDIzxLHVVc7CeFhyQ7NEofsBNhRwjrnchA1onNOMwoQ/xU4k } 13, 53 -- GlNT9VefdH3phrwGEcxoF+YUtwv8 } 13, 53 -- X-Google-Smtp-Source: AGs4zMa17Mah5ciiqmYbjFsgBJIMp4Q9FQSWDIx5LCnxz99Kp3+HdN2/YzJUDJMfrLF91MumAUhpXQ== } 13, 53 -- X-Received: by 10.84.193.129 with SMTP id f1mr6807886pld.317.1510960529479; } 13, 53 -- Fri, 17 Nov 2017 15:15:29 -0800 (PST) } 13, 53 -- Received: from Nestors-MacBookAir-Pro-2014-ElCapitan-OSX-1163.local ([182.16.234.14]) } 13, 53 -- by smtp.googlemail.com with ESMTPSA id p19sm9352707pfj.140.2017.11.17.15.15.24 } 13, 53 -- for {microscopy-at-microscopy.com} } 13, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 13, 53 -- Fri, 17 Nov 2017 15:15:24 -0800 (PST) } 13, 53 -- Subject: viaWWW:EDS and SEM projector for Outreach } 13, 53 -- References: {201711162229.vAGMTWp6026431-at-microscopy.com} } 13, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 13, 53 -- From: Microscopy Listserver {microscopy.listserver-at-gmail.com} } 13, 53 -- X-Forwarded-Message-Id: {201711162229.vAGMTWp6026431-at-microscopy.com} } 13, 53 -- Message-ID: {4230175d-0ecf-bad3-c4f0-4f12f99a8f95-at-gmail.com} } 13, 53 -- Date: Sat, 18 Nov 2017 07:15:22 +0800 } 13, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) } 13, 53 -- Gecko/20100101 Thunderbird/52.4.0 } 13, 53 -- MIME-Version: 1.0 } 13, 53 -- In-Reply-To: {201711162229.vAGMTWp6026431-at-microscopy.com} } 13, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 13, 53 -- Content-Language: en-US } 13, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
--
J. Faerber IPCMS-DSI Institut de Physique et Chimie des Matériaux de Strasbourg Département Surfaces et Interfaces 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
The Gatan MonoCL uses a mirror arrangement to direct the light to the optics. It may be that the longer wavelengths (red-orange) need a different geometry for optimisation of the collection. I would try and move the sample height and see if the spectra sensitivity changes with respect to the red end of the spectrum. The MonoCl systems typically have a PMT detector to detect the light and these usually have adjustable gain. Since the longer wavelengths are energetically weak you may have to increase the gain to detect these less energetic wavelengths.
Regards Colin
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Saturday, 18 November 2017 10:49 AM To: MacRae, Colin (Mineral Resources, Clayton) {Colin.Macrae-at-csiro.au}
X-from: fay1-at-email.arizona.edu
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both fay1-at-email.arizona.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] SEM-CL missing red wavelengths?
Message: Dear Microscopy Community:
Does anybody know why an SEM-CL instrument could be missing the red-to-orange wavelengths of the cathodoluminescence spectrum?
I run a JEOL 6010LA benchtop SEM wit a recently acquired Gatan MonoCL4 cathodoluminescence spectrometer. It works great for zircons, quartz, etc., but picks up little to no signal in the red to orange part of the spectrum even on samples that glow brilliant red under our ancient optical cold-cathode Luminoscope. I checked the same samples out on a ChromaCL hooked up to a Hitachi S3400 in another lab, and there is a similar lack of red signal. I've checked and the accelerating voltages on the Luminoscope are about the same as on the SEM, although the Luminoscope has higher beam current, so I'm not sure why the SEM-CL instruments don't pick up the red luminescence. I've read that filters placed in front of a detector can eliminate red-orange luminescence and phosphorescence (Reed and Milliken, Journal of Sedimentary Research 73.2: 328-332). There was no mention of such a filter in the Gatan datasheets for either the ChromaCL or the MonoCL4, but that seems to me to be one possible explanation for the lack of red luminescence in SEM-CL compared to optical cold-cathode CL. I've left a message with Gatan but haven't heard back yet. Has anybody else with SEM-CL encountered a similar problem, or know of any fixes? We got the MonoCL4 partly in order to look at carbonate cements in rocks, so it's pretty important that we figure out how to get good spectra and images in the red and orange zones.
Any help would be appreciated. Thanks,
Isabel Barton
Login Host: 68.106.21.127 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
It is always fun to present science to young minds!
I would suggest table salt. Nice crystal structure for morphology and an easy spectrum. I've also done a comparison with table salt and natural salts (Sea or Himalayan) which can show differences in both morphology and spectra.
Have fun!
Gene Eugene Rodek Vice President 206 Garfield Ave West Chester, PA 19380 610-436-5400 x109 erodek-at-2spi.com www.2spi.com ________________________________________ X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Friday, November 17, 2017 6:46 PM To: Gene Rodek
X-from: annie.muske-dukes-driggs-at-lonza.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both annie.muske-dukes-driggs-at-lonza.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] EDS and SEM projector for Outreach
Message: Hi everyone,
My company runs a community outreach program for local elementary school students (8-10 years old). They tour our facility and see quick demonstrations/lectures on a variety of topics. We have had an SEM demo for years and I would like to incorporate our EDS but I'm having trouble coming up with samples that kids that age understand, especially since I only have 15 minutes per group and we try to go through at least four samples.
I have generated a map of different elements in a vitamin tablet which went over well and gives them a good understanding of what the system is doing. Any recommendations for samples that generate interesting maps or have unexpected elements? I'll be doing the prep/high quality maps ahead of time so that doesn't need to be done in the 15 minutes.
I'd also like to get a wireless projector to use with our Hitachi SU3500 to project a live image of the screen onto the wall. Our service rep said some models can cause driver issues so if anyone here has a model that works please let me know. We need to stay under US$500, but under $300 would be ideal.
Thanks, Annie (I am a list subscriber but our required corporate attachments prevent me from sending the usual way)
Login Host: 64.47.109.63 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
On Friday, November 17, 2017, {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote:
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver On-Line {http://www.microscopy.com/MicroscopyListserver On-Line} Help http://www.microscopy.com/MicroscopyListserver/FAQ.html {http://www.microscopy.com/MicroscopyListserver/FAQ.html} ----------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html {http://www.microscopy.com/MLFormMail.html} --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both annie.muske-dukes-driggs-at-lonza.com {javascript:;} as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] EDS and SEM projector for Outreach
Message: Hi everyone,
My company runs a community outreach program for local elementary school students (8-10 years old). They tour our facility and see quick demonstrations/lectures on a variety of topics. We have had an SEM demo for years and I would like to incorporate our EDS but I'm having trouble coming up with samples that kids that age understand, especially since I only have 15 minutes per group and we try to go through at least four samples.
I have generated a map of different elements in a vitamin tablet which went over well and gives them a good understanding of what the system is doing. Any recommendations for samples that generate interesting maps or have unexpected elements? I'll be doing the prep/high quality maps ahead of time so that doesn't need to be done in the 15 minutes.
I'd also like to get a wireless projector to use with our Hitachi SU3500 to project a live image of the screen onto the wall. Our service rep said some models can cause driver issues so if anyone here has a model that works please let me know. We need to stay under US$500, but under $300 would be ideal.
Thanks, Annie (I am a list subscriber but our required corporate attachments prevent me from sending the usual way)
Login Host: 64.47.109.63 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
I believe Colin is correct about finding the right focal point with the GatanCL. We also have this detector on a Hitachi 3400, and have no problems with the red signal. However, finding the right focal point can be essential. 0.5 mm adjustment in the Z axis or less can make a big difference in signal strength.
Phil ------------- Philip Oshel Imaging Facility Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 989 774-3576 office 989 774-7567 lab
The Gatan MonoCL uses a mirror arrangement to direct the light to the optics. It may be that the longer wavelengths (red-orange) need a different geometry for optimisation of the collection. I would try and move the sample height and see if the spectra sensitivity changes with respect to the red end of the spectrum. The MonoCl systems typically have a PMT detector to detect the light and these usually have adjustable gain. Since the longer wavelengths are energetically weak you may have to increase the gain to detect these less energetic wavelengths.
Regards Colin
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Saturday, 18 November 2017 10:49 AM To: MacRae, Colin (Mineral Resources, Clayton) {Colin.Macrae-at-csiro.au}
X-from: fay1-at-email.arizona.edu
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both fay1-at-email.arizona.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] SEM-CL missing red wavelengths?
Message: Dear Microscopy Community:
Does anybody know why an SEM-CL instrument could be missing the red-to-orange wavelengths of the cathodoluminescence spectrum?
I run a JEOL 6010LA benchtop SEM wit a recently acquired Gatan MonoCL4 cathodoluminescence spectrometer. It works great for zircons, quartz, etc., but picks up little to no signal in the red to orange part of the spectrum even on samples that glow brilliant red under our ancient optical cold-cathode Luminoscope. I checked the same samples out on a ChromaCL hooked up to a Hitachi S3400 in another lab, and there is a similar lack of red signal. I've checked and the accelerating voltages on the Luminoscope are about the same as on the SEM, although the Luminoscope has higher beam current, so I'm not sure why the SEM-CL instruments don't pick up the red luminescence. IÂ've read that filters placed in front of a detector can eliminate red-orange luminescence and phosphorescence (Reed and Milliken, Journal of Sedimentary Research 73.2: 328-332). There was no mention of such a filter in the Gatan datasheets for either the ChromaCL or the MonoCL4, but that seems to me to be one possible explanation for the lack of red luminescence in SEM-CL compared to optical cold-cathode CL. I've left a message with Gatan but haven't heard back yet. Has anybody else with SEM-CL encountered a similar problem, or know of any fixes? We got the MonoCL4 partly in order to look at carbonate cements in rocks, so itÂ's pretty important that we figure out how to get good spectra and images in the red and orange zones.
Any help would be appreciated. Thanks,
Isabel Barton
Login Host: 68.106.21.127 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
Hi Annie, I usually show coins and matches (new and burnt) for similar purpose. Coins for oxidation (Copper coin) and metal allergies (Ni alloy). Matches produce nice elemental maps of Cl, K, Na.
Regards,
Elio
2017-11-18 0:53 GMT+01:00 {microscopy.listserver-at-gmail.com} : } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: annie.muske-dukes-driggs-at-lonza.com } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both annie.muske-dukes-driggs-at-lonza.com as well } as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: annie.muske-dukes-driggs-at-lonza.com Name: Annie Muske-Dukes } } Organization: Bend Research } } Title-Subject: [Filtered] EDS and SEM projector for Outreach } } Message: Hi everyone, } } My company runs a community outreach program for local elementary school } students (8-10 years old). They tour our facility and see quick } demonstrations/lectures on a variety of topics. } We have had an SEM demo for years and I would like to incorporate our } EDS but I'm having trouble coming up with samples that kids that age } understand, especially since I only have 15 minutes per group and we try } to go through at least four samples. } } I have generated a map of different elements in a vitamin tablet which } went over well and gives them a good understanding of what the system is } doing. Any recommendations for samples that generate interesting maps or } have unexpected elements? I'll be doing the prep/high quality maps ahead } of time so that doesn't need to be done in the 15 minutes. } } I'd also like to get a wireless projector to use with our Hitachi SU3500 } to project a live image of the screen onto the wall. Our service rep } said some models can cause driver issues so if anyone here has a model } that works please let me know. We need to stay under US$500, but under } $300 would be ideal. } } Thanks, } Annie (I am a list subscriber but our required corporate attachments } prevent me from sending the usual way) } } Login Host: 64.47.109.63 } Listserver Email Form V - 20120416 } ---------------------------------------------------------------------------
==============================Original Headers============================== 6, 39 -- From sem.nattabg-at-gmail.com Mon Nov 20 14:54:40 2017 6, 39 -- Received: from mail-it0-f46.google.com (mail-it0-f46.google.com [209.85.214.46]) 6, 39 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id vAKKsewN018282 6, 39 -- for {microscopy-at-microscopy.com} ; Mon, 20 Nov 2017 14:54:40 -0600 6, 39 -- Received: by mail-it0-f46.google.com with SMTP id m191so13377725itg.2 6, 39 -- for {microscopy-at-microscopy.com} ; Mon, 20 Nov 2017 12:51:24 -0800 (PST) 6, 39 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 39 -- d=gmail.com; s=20161025; 6, 39 -- h=mime-version:from:date:message-id:subject:to; 6, 39 -- bh=SagId9kG/v7nDLasn4CiOLTb+Q1wTnGRA9sWAcvSLUQ=; 6, 39 -- b=XclDXYzfq/vz1IvIkR4yEF2BUAWClI4Yi7BDi5agU2+F7hUD1+Xutu1gVfBaspTuru 6, 39 -- /5cNNkt2fK6pYcx81Hw5uihGXdDEBZY9+0Zn5kRkVDBeY/0eXe4uzWWsLGynTWsnEPoY 6, 39 -- my8pbZh6gFL6Sc8xh+Ojg8lJsIKJwgidTdSoCEAUdsPehvGQHZMrhuQgCYNGzyC0kGqX 6, 39 -- qT4w+sadQcFg5GKPxKi6h70vAVHq9aBHe5AHTK8Fvc9/yBbAFy4tK4PhlVBgo/fO+NsP 6, 39 -- phrIaJD8bsPY5Pq7/r0auAlVugEC6fRKUCFcUJBlwsyjboCDshg5jfCkHZnSBSuwKJjQ 6, 39 -- KY6w== 6, 39 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 39 -- d=1e100.net; s=20161025; 6, 39 -- h=x-gm-message-state:mime-version:from:date:message-id:subject:to; 6, 39 -- bh=SagId9kG/v7nDLasn4CiOLTb+Q1wTnGRA9sWAcvSLUQ=; 6, 39 -- b=T6k8fGh4Cq9SFgxofAM+wu6wcGXWjrCHDj3evaDLnGQCTbc2maReud5pObboUfRcvI 6, 39 -- lt0j8rkeI9Pnhq5Xg4nO1ugCo6KRj3cK2j/ZLr8TVjYhfqNoVDbcElBwCWVkYB+qy1au 6, 39 -- DCpDMwBeTE1FtZEZAkXjI+L5ge/eA+3+VDqu88yHn/q2fGUm/UPwJJpXMTBNAR+PWH1E 6, 39 -- gu3PdgtWqfH39e3CrgpLPnJVM/Kex2D1/if3ubi9KEfbE3RaUCKuZdNC6IxyWwmHsI0L 6, 39 -- d5b5l7otypdmA6JfTYdGY4KRss0Hpt/KN68RyGYVaghOtnKg9q16HSiFsOxUfMCy/XGs 6, 39 -- uvgQ== 6, 39 -- X-Gm-Message-State: AJaThX4gkvc4/ODq+DtC90zEcY8zWfis2oKuAw7rDW/+ZsGNaIAVOiQ1 6, 39 -- nXfDm7w72xUbAV3aKy2o1MKHItgi0EIaCmS05Ic3iw== 6, 39 -- X-Google-Smtp-Source: AGs4zMbfJ+f2dMZDLBWMwPJH8zBmMOciJJUq04YvUnzU+lQQ/utcjBUrMT/6eNMoksS7WrOcIFIU6e8xGtxpyekdVWY= 6, 39 -- X-Received: by 10.36.76.7 with SMTP id a7mr20738248itb.35.1511211083940; Mon, 6, 39 -- 20 Nov 2017 12:51:23 -0800 (PST) 6, 39 -- MIME-Version: 1.0 6, 39 -- Received: by 10.2.163.153 with HTTP; Mon, 20 Nov 2017 12:51:23 -0800 (PST) 6, 39 -- From: Sem Natta {sem.nattabg-at-gmail.com} 6, 39 -- Date: Mon, 20 Nov 2017 21:51:23 +0100 6, 39 -- Message-ID: {CAMkPEzgar3u6SmZbrncrGGRJ_swemhSxCCiuxm6_EwPQo8Fq=A-at-mail.gmail.com} 6, 39 -- Subject: [Microscopy] viaWWW:EDS and SEM projector for Outreach 6, 39 -- To: microscopy-at-microscopy.com 6, 39 -- Content-Type: text/plain; charset="UTF-8" ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jerry.biehler-at-gmail.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: jerry.biehler-at-gmail.com Name: Jerry Biehler
Message: This is a bit off topic but Im thowing this out there hoping someone might have some leads. I have a QE system from SITe that I am trying to work some bugs out of. It is composed of a Optronic Laboratories 740A/D Monochromater controlled with a 740-1C wavelength drive and a 730A Radiometer amp/readout.
Trying to find the manuals or at least the controller/BCD port specs for the wavelength drive and radiometer.
Gooch & Housego took them over but it is looking like they may not have any info on these old units anymore. Thanks
-Jerry
Login Host: 204.153.43.31 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
I usually deal with kids in the 12 - 18 YO range for outreach. In the past we offered a week long half day experience in the lab. In my experience it is critical to find samples that kids can relate to.
Insects can be a good for imaging. My favorites are honey bees, house fly's and fruit fly's. Kids really like the structure of the eye.
Epsom salt has a nice structure and is useful for EDX; however, it can take a long time to get vacuum since it is usually hydrated. Best to only use a few crystals or prepump.
Geological samples. I prefer a thin section to reduce pump time, but bulk sections work too. If you need a thin section I suspect Spectrum Petrographics could help. I have no financial connection to them, but I know several of our geology Professors have Spectrum do their mounts.
I had one student bring hair and feathers from the farm she lives on. There is a significant difference between animals.
House hold dust can be a good sample. Just pick a spot like a window sill and don't clean it for a week or two. Collect the sample with C tape on a stub. I have seen pollen, insect parts and silicate minerals.
Greg
On Fri, Nov 17, 2017 at 3:49 PM, {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote:
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver On-Line {http://www.microscopy.com/MicroscopyListserver On-Line} Help http://www.microscopy.com/MicroscopyListserver/FAQ.html {http://www.microscopy.com/MicroscopyListserver/FAQ.html} ----------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html {http://www.microscopy.com/MLFormMail.html} --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both annie.muske-dukes-driggs-at-lonza.com {mailto:annie.muske-dukes-driggs-at-lonza.com} as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] EDS and SEM projector for Outreach
Message: Hi everyone,
My company runs a community outreach program for local elementary school students (8-10 years old). They tour our facility and see quick demonstrations/lectures on a variety of topics. We have had an SEM demo for years and I would like to incorporate our EDS but I'm having trouble coming up with samples that kids that age understand, especially since I only have 15 minutes per group and we try to go through at least four samples.
I have generated a map of different elements in a vitamin tablet which went over well and gives them a good understanding of what the system is doing. Any recommendations for samples that generate interesting maps or have unexpected elements? I'll be doing the prep/high quality maps ahead of time so that doesn't need to be done in the 15 minutes.
I'd also like to get a wireless projector to use with our Hitachi SU3500 to project a live image of the screen onto the wall. Our service rep said some models can cause driver issues so if anyone here has a model that works please let me know. We need to stay under US$500, but under $300 would be ideal.
Thanks, Annie (I am a list subscriber but our required corporate attachments prevent me from sending the usual way)
Login Host: 64.47.109.63 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
X-from: Ferenc Molnar {ferenc.l.molnar-at-googlemail.com}
Dear Erico,
this sounds to me as there is an issue with additional light in the chamber. Make sure that all light sources - especially chamber scope - are turned off. A little bit tricky might be a touch alarm: on some SEM (do we talk about SEM?) the stage alarm triggers some safety light barrier in order to prevent another collision. To turn these lights off, you have to initialize the stage.
If you can exclude additional light in the chamber, there is maybe an issue with the detector: it can be the cooling system or some issue with the preamp of the SDD/SiLi.
I hope that helps. In general, EDS detectors are very sensible to all kind of light, not only the Xray you want to see.
With best regards, Ferenc
{microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } schrieb am So., 19. Nov. 2017 um 16:38 Uhr:
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver On-Line {http://www.microscopy.com/MicroscopyListserverOn-Line} Help http://www.microscopy.com/MicroscopyListserver/FAQ.html ----------------------------------------------------------------------------
Our EDS is showing a high intensify peak at about 0.2 keV even with beam on vacuum. We're not getting C signal anymore and the energy resolution is getting worse. There'is been an energy shift also. The issue remains after run an automatic callibraion. Does anybody know what more could be done? Or is it a sign that our detector is almost dead?
Regards,
Erico Freitas Físico/Physicist Centro de Microscopia Universidade Federal de Minas Gerais
Coordenador do Lab. de Microscopia Eletrônica dr Transmissão
I did a CSI type demo using light bulb filaments. The scenario was that there was a traffic accident in the dark and each driver claimed the others headlights were not on. The headlamps in both cars were broken. The 2nd driver claims that the 1st driver turned on his headlights after the bulbs were broken in order to cover his fault. The officer collected the light bulb filaments from the cars and you are comparing them in the SEM. (You can spin out the story as much as you want!)
I took 2 small incandescent bulbs (15 watt if I remember), broke the glass on one and then applied power so that it burned out. I took the 2nd and broke the glass while it was running. I then mounted the 2 filaments on an SEM sticky tab and put them in the scope.
Note: be careful about breaking the glass. Do it safely! I wore safety glasses and leather gloves and used a pair of channel pliers to hold the bulb inside a mostly closed cardboard box to contain the glass fragments.
On examination, the student will find small spherical globs on one filament and none on the other. Doing EDX shows that the spherical globs are Si and O (i.e. glass that melted). The inference is that the filament was hot when the glass was broken, hence the light bulb was on. The other bulb is just oxidized and doesn't show any melted glass. Hence it was not running when the glass broke and was burned out afterwards.
They seemed to enjoy the "real life" problem solving.
Cheers, Henk
--------------------
Hendrik O. Colijn Center for Electron Microscopy and AnalysiS The Ohio State University 1305 Kinnear Rd, Suite 100, Columbus, OH 43212
colijn.1-at-osu.edu 614/643-3458 cemas.osu.edu
"Time is that quality of nature which keeps things from happening all at once." (Ray Cummings - 1922) Lately it doesn't seem to be working.
------ Original Message ------ X-from: microscopy.listserver-at-gmail.com To: colijn.1-at-osu.edu Sent: 11/20/2017 8:38:57 PM
I just found these on Ebay- is there anyone out there with the passion/free time/garage space to rescue these before they get scrapped? I just hate seeing these old scopes go to scrappers…
==============================Original Headers============================== 3, 49 -- From kraftpiano-at-gmail.com Tue Nov 21 09:40:49 2017 3, 49 -- Received: from mail-qk0-f194.google.com (mail-qk0-f194.google.com [209.85.220.194]) 3, 49 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id vALFenQR006368 3, 49 -- for {microscopy-at-microscopy.com} ; Tue, 21 Nov 2017 09:40:49 -0600 3, 49 -- Received: by mail-qk0-f194.google.com with SMTP id b85so12580397qkc.13 3, 49 -- for {microscopy-at-microscopy.com} ; Tue, 21 Nov 2017 07:37:35 -0800 (PST) 3, 49 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 49 -- d=gmail.com; s=20161025; 3, 49 -- h=from:content-transfer-encoding:mime-version:subject:message-id:date 3, 49 -- :to; 3, 49 -- bh=CRe3mzA+e92pDhuMw/yEiOSpyK/S3tn1dd9TQz1ZpdY=; 3, 49 -- b=IS574AoiOorhrEY98GoL8SwP2rMNuqDBn/3X/dXY8x29uavdtNp6txy1ZZJhb03dBc 3, 49 -- NXvEaBd7S5YDqCw2Eg3y6D2cTts9d6bsZdtREPb6uzXxBSKFgegahENoX2WyuDkdAhJh 3, 49 -- O0l4eOV56oqjHzm2k8VyHZqMxSAJgLNhfChrjv6oZ2hpjLSHw2ybh936p5MNzbUq2PCq 3, 49 -- arZe+LTIbMlgIGdVsk0HcCunlD4ZpT5g23Coc7wXyexcm/YMjVlM8lkna8X7FuvI6iqk 3, 49 -- 1fhGKgxw9y7kzFt9FIlJei9C8mcXb+beT/Kzj/XkdwdDIdQ1p1+045zJjV5ieZvqAmQG 3, 49 -- p9iw== 3, 49 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 49 -- d=1e100.net; s=20161025; 3, 49 -- h=x-gm-message-state:from:content-transfer-encoding:mime-version 3, 49 -- :subject:message-id:date:to; 3, 49 -- bh=CRe3mzA+e92pDhuMw/yEiOSpyK/S3tn1dd9TQz1ZpdY=; 3, 49 -- b=eRHYl3Y31bjcuSBxaxUtBj8pCc8JpNbFU/QgM82raK/RXz915BKlwsdIvBV6Wp7zhp 3, 49 -- 3Z/eXCSwywUfrrzNttCAUGCaC9OeFUP6e3AiQHXpk8EJbmJjXjYk2nD5HbD77c4CnjjG 3, 49 -- dHqrmrQeW9+kc7P1RE2rbvDKnqcX6XdA2AO0oqEh94fU9gj9SyZzJNr35rlAsBTLZIkg 3, 49 -- ffHVI6hHoetsjwPVTYU2myMhHDque3vwpwENKGsaDcehVU5yHRqKN3mgSdUNMw2O5G2N 3, 49 -- z8q0/uXvlHMo9UF2V7W1ZizS22K4Sc9ZsFYM3aozIzI0eV8nHFsUHmTzEyOZHK3tYxlz 3, 49 -- nw0w== 3, 49 -- X-Gm-Message-State: AJaThX4YtCH3fr0OoM4WarJvMdd3CDm3bstuv6xaRRIgOZdKhLK+aDTu 3, 49 -- TVRBCNqGv3gG2x/rW/Ra9qpMc1Je 3, 49 -- X-Google-Smtp-Source: AGs4zMa6k6Dd7wKSXNIWYpnlzLF8m3/uowFQ5DaVKFmdrZ9kyqnZoiW9D6edf2oZjewwGV2TT+65wg== 3, 49 -- X-Received: by 10.55.22.138 with SMTP id 10mr27553098qkw.16.1511278654735; 3, 49 -- Tue, 21 Nov 2017 07:37:34 -0800 (PST) 3, 49 -- Received: from justin.jkraft.net (rrcs-208-125-225-220.nys.biz.rr.com. [208.125.225.220]) 3, 49 -- by smtp.gmail.com with ESMTPSA id i42sm9227319qtf.61.2017.11.21.07.37.34 3, 49 -- for {microscopy-at-microscopy.com} 3, 49 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 3, 49 -- Tue, 21 Nov 2017 07:37:34 -0800 (PST) 3, 49 -- From: Justin Kraft {kraftpiano-at-gmail.com} 3, 49 -- Content-Type: text/plain; 3, 49 -- charset=utf-8 3, 49 -- Mime-Version: 1.0 (Mac OS X Mail 11.1 \(3445.4.7\)) 3, 49 -- Subject: Vintage rescue on eBay? 3, 49 -- Message-Id: {93D7EA47-174B-4FD7-8F4C-E6B3E273F321-at-gmail.com} 3, 49 -- Date: Tue, 21 Nov 2017 10:37:33 -0500 3, 49 -- To: microscopy-at-microscopy.com 3, 49 -- X-Mailer: Apple Mail (2.3445.4.7) 3, 49 -- Content-Transfer-Encoding: 8bit 3, 49 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id vALFenQR006368 ==============================End of - Headers==============================
From carlemau296wvuy-at-gmail.com Wed Nov 22 04:33:03 2017 Return-Path: {carlemau296wvuy-at-gmail.com} Received: from gmail.com ([45.115.242.244]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id vAMAX2Et022864 for {microscopylistserverarchive6-at-microscopy.com} ; Wed, 22 Nov 2017 04:33:03 -0600 Received: from external.newsubdomain.com ([213.142.107.125]) by mail.webhostings4u.com with SMTP; Wed, 22 Nov 2017 07:24:11 -0300 Received: from unknown (90.246.26.219) by webmail.halftomorrow.com with NNFMP; Wed, 22 Nov 2017 07:17:53 -0300 Received: from mail.naihautsui.co.kr ([60.28.7.69]) by qnx.mdrost.com with LOCAL; Wed, 22 Nov 2017 07:05:12 -0300 Received: from mail.webhostings4u.com ([142.12.69.200]) by mailout.endmonthnow.com with ASMTP; Wed, 22 Nov 2017 06:49:20 -0300 Message-ID: {87632980.17F6B084-at-gmail.com}
X-from: shiree.thomas-at-unsw.edu.au
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both shiree.thomas-at-unsw.edu.au as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: shiree.thomas-at-unsw.edu.au Name: Shiree Thomas
Organization: UNSW Sydney
Title-Subject: [Filtered] Job Vacancy - Associate Director, Electron Microcope Unit - Sydney, Australia
Message: Academic and Associate Director level position for candidate with electron microscopy expertise and independent research portfolio (materials science preferred)at UNSW Sydney. Associate Professor/Senior Lecture level five-year appointment with options for renewal Make a real impact! Influence and support advancements in electron microscopy Enjoy state-of-the-art facilities and collaboration with interdisciplinary researchers
For further information and to view the position description please click on the link below: https://applicant.cghrm.unsw.edu.au/psp/hrm/NS_CAREERS/HRMS/c/HRS_HRAM.HRS_APP_SCHJOB.GBL?Page=HRS_APP_JBPST&Action=U&FOCUS=Applicant&SiteId=1&JobOpeningId=58234&PostingSeq=1
Contact: Professor Richard Tilley Director Electron Microscope Unit and AMMRF node Mark Wainwright Analytical Centre, Division of Research T +61 (2) 9385 4435 E r.tilley-at-unsw.edu.au
Login Host: 129.94.40.90 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jhyun-at-gatan.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Message: Gatan, Inc. is currently seeking a skilled CL Application Scientist who will be responsible for supporting the sales and marketing efforts of Gatans highly successful Cathodoluminescence (CL) product line. The position is based at Gatans Pleasanton, CA headquarters but will support CL products worldwide.
Primary duties of this role will include:
Providing pre-sales applications support with presentations and demonstrations of Gatan products on a range of SEM and TEM models. Driving the development of new applications by working closely with key customers, presenting at scientific conferences and writing scientific articles. Providing post-sales customer support and on-site customer training. Tasks include: general applications support, phone/email support, and limited hardware and software troubleshooting. Proposing design enhancements and improvements to Gatan hardware and software. Working with Gatan R&D in the development and testing of new products and applications. The successful incumbent will have: Ability to interface effectively with customers at all experience levels while projecting a strong client service attitude. Excellent verbal, written, and interpersonal communication skills in English are essential. Ability to work on complex learning and development problems as well as teach highly technical information is essential.
Requirements: Advanced degree in science or engineering (or equivalent experience) is required. Strong background in electron microscopy (SEM and/or TEM), specifically in relation to the acquisition, application, interpretation and, discussion of CL. Hands-on, post-graduate level experience in advanced CL applications with a proven publication record. Ability to manage approximately 50% both domestic and international travel required. To apply to this opportunity or to view our other current job openings, please visit www.gatan.com/careers. Equal Opportunity Employer/Protected Veterans/Individuals with Disabilities
Login Host: 50.58.128.11 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
There is a permanent TEM specialist position opening at the Imaging and Characterization Core Lab of King Abdullah University of Science and Technology (KAUST) in Saudi Arabia. Please find the detailed information below. KASUT offers competitive package and balanced work/life environment. If interested, please send your CV to kun.li-at-kaust.edu.sa
Regards,
Kun Li
King Abdullah University of Science and Technology is an international graduate-level, merit-based research university located on the shores of the Red Sea in Saudi Arabia. Our state-of-the-art campus, globally renowned faculty and cutting-edge facilities come together to provide the ideal setting for significant, high-impact research. KAUST is dedicated to inspiring a new age of scientific achievement in the Kingdom that will also benefit the region and the world.
*Major Responsibilities * ·Perform standardized protocols for histology ·Handle research specimens based on sound knowledge of histological/immunochemical processes. ·TEM sample preparation and TEM imaging with focus in the field of biology ·Maintain and run EM preparation lab ·Engage in KAUST research projects with senior staff to provide value-added service ·Ensure maintenance status and maximum uptime of assigned instruments (plan, perform and oversee preventative and corrective maintenance, purchase consumables, etc.) ·Keep safe working procedure, in compliance with KAUST HSE regulations. ·Other duties as assigned by the team lead and lab director *Competencies * ·Good knowledge of cell biology and cell structure ·General understanding and expertise in cell/tissue fixation, processing, embedding, sectioning and staining. ·Ability to operate common sample preparation tools independently ·Hands-on experience on EM sample preparation ·Hands-on experience on Ultramicrotome ·Working experience with cell culture ·Experience with cryo electron microscopy preferred ·Good communication and inter-personal skills *Qualifications* ·A master’s degree in the life science or biochemistry-related field
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Ziqiang.Huang-at-cruk.cam.ac.uk as well as the Microscopy Listserver ---------------------------------------------------------------------------
Does any of you have experience (or just an idea will be also helpful) using Zeiss Orion in biology (or life science) researches, preferably in cancer research? I used the Zeiss Auriga system before to quantify synapses in a small brain volume, but not very familiar with the Orion system. I will be very interested in knowing what kind of biology projects the Orion system can potentially help.
Best Regards. Ziqiang
Bioimage Analyst of Light Microscopy Facility CRUK Cambridge Institute
Login Host: 131.111.84.208 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
From jeverett397rxqi-at-gmail.com Sat Nov 25 16:26:06 2017 Return-Path: {jeverett397rxqi-at-gmail.com} Received: from gmail.com ([121.201.38.118]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id vAPMQ4un026907 for {microscopylistserverarchive6-at-microscopy.com} ; Sat, 25 Nov 2017 16:26:05 -0600 Received: from snmp.otwaloow.com ([22.144.65.170]) by mtu67.syds.piswix.net with ASMTP; Sat, 25 Nov 2017 15:59:53 -0500 Received: from relay37.vosimerkam.net ([Sat, 25 Nov 2017 15:51:25 -0500]) by smtp-server1.cfdenselr.com with NNFMP; Sat, 25 Nov 2017 15:51:25 -0500 Received: from [143.149.145.121] by smtp4.cyberemailings.com with QMQP; Sat, 25 Nov 2017 15:40:11 -0500 Received: from unknown (47.49.100.138) by mts.locks.grgtween.net with ASMTP; Sat, 25 Nov 2017 15:31:02 -0500 Message-ID: {22F5BA07.A9EB7B40-at-gmail.com}
Dear List Thanks to everybody provided ideas about the problem. We found a faulty voltage regulator in the HT I/O board. This regulator provides a 39V power rail to the HT Drive board, used by the filament current generating circuit. The regulator had failed short resulting in an overvoltage of some 70V going to the filament circuitry, probably causing more damage there (we replaced the regulator but it was destroyed instantly). Finally we replaced the faulty voltage regulator, the HT drive board and the HV power supply in the oil tank. I was lucky to have spares of the last two. The microscope now works fine.
Only thing that we still try to solve is that the readings on the SEM menu regarding gun alignment values seem to be incorrect. Also when we turn HT on, the value of the current fluctuates for several seconds before stabilizing to the expected value. Since the HT board and power supply are coming from another microscope, it may need some sort of reset that we haven't found yet. Has anybody any clue?
Best regards yorgos
Dear List Our SEM JSM5600LV burns filaments immediately upon turning high tension ON. There was a thunderstorm here and after a big thunder the microscope went completely OFF while there was no power cut. Very soon the microscope's power came back and worked normally but I noticed the image was bit noisy and definition less high. Then I switched it off overnight and today I started and realized the problem (after I burned a couple of filaments). Any comment or suggestion will be greatly appreciated
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both sgkcck-at-aol.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Message: Electron Microscopy Sciences is pleased to offer the Aurion Immuno Gold Silver Staining Workshop, led by expert Peter van de Plas, at our new facility - the EMS Microscopy Academy!
The objective of the course is to provide researchers with the opportunity to learn the theory and practice of Immuno Gold labeling. Participants will process their own samples under the expert guidance of our tutors, who are experts in Immuno Gold Silver Staining techniques.
During the workshop attendees will receive theory, including but not limited to immune detection, in situ hybridization, silver enhancement, as well as background issues. There will be time for practice, as well. Attendees will be able to work with their own specimens, as well as ones we will have prepared. A full review of incubation methods, testing of antigenicity and reactivity, complete principles of Immuno Gold labeling, and preparation of conjugates for EM and LM will be covered. For more information, copy and paste this link into your browser! http://www.emsdiasum.com/microscopy/academy/courses/immunogold2.aspx
or contact me at sgkcck-at-aol.com
Thank you for your support!
Stacie Kirsch
Login Host: 73.233.226.182 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both walbrid-at-cshl.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: walbrid-at-cshl.edu Name: Samantha
Organization: Cold Spring Harbor Laboratory
Title-Subject: [Filtered] Research Associate - Electron Microscopy Technologist
Message: Cold Spring Harbor Laboratory seeks a highly motivated dedicated individual to work in a state-of-the-art Microscopy Shared Resource.
The individual should have extensive practical expertise in biological sample preparation for transmission and scanning electron microscopy of animal tissues and mammalian cell lines. Hands-on knowledge of confocal and widefield fluorescence microscopy would also be a plus.
The candidate will help users design innovative experiments and they will carry out sample preparation and imaging as well as assist in data interpretation.
Excellent verbal and written communication skills, ability to work with multiple users in a supporting role, and ability to work independently and proactively with limited supervision are essential. A Bachelors degree in biology or related discipline is required. One to three years of experience working in a Microscopy Shared Resource is preferred.
How to Apply Interested individuals should apply for this position via the CSHL careers website at http://cshl.peopleadmin.com/postings/11688
Position Number 01779-R
Applicants should include a resume along with a description of their practical expertise and the names as well as email addresses of 3 references.
Cold Spring Harbor Laboratory is a world-renowned research and educational institution recognized internationally for its excellence in ground-breaking research programs in cancer, neuroscience, plant biology, genomics, and bioinformatics and broad educational mission.
For more information about CSHL, please visit us at www.cshl.edu
CSHL is an EO/AA Employer. All qualified applicants will receive consideration for employment and will not be discriminated against on the basis of race, color, religion, sex, sexual orientation, gender identity, national origin, age, disability or protected veteran status.
VEVRAA Federal Contractor
Login Host: 143.48.14.89 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
We’re currently in the process of setting up a new JEOL GrandARM instrument and we are looking at spray on foam insulation as a possible way to dampen sound inside the scope room. I have found several different manufacturers focused on reducing sound in recording studios, auditoriums, etc.
Does anyone have experience with the use of such insulation? How effective is it and how does it compare to sound dampening ceiling tiles (e.g. tectum)?
Thanks for your help! ______________________________________ Steven R. Spurgeon, Ph.D. Staff Scientist Energy and Environment Directorate
Pacific Northwest National Laboratory 902 Battelle Boulevard P.O. Box 999 MSIN:P7-25 Richland, WA 99352
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both keith.prater-at-solvay.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: keith.prater-at-solvay.com Name: Keith Prater
Does anyone have a detailed procedure that you'd be willing and allowed to share for pre-thinning specimens using the Gatan Disc Grinder 623? It would be very much appreciated as I have found only limited descriptions online.
Thank you! Login Host: 150.251.3.1 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both bonnie_salyer-at-tedpella.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Join our Team! Message: Live and work in one of the most beautiful areas in California Redding. Ted Pella Inc. is seeking a Materials Science Product Specialist to be responsible product development and sales/marketing activities related to our SEM, Materials Science, Forensic and AFM product lines. This is an exciting time for this position as we recently acquired additional product lines associated with the semi-conductor industry. Must have BS in Materials Science or related, 5 years hands on SEM instrument use and specimen prep experience, and 2 years TEM instrument use and specimen prep experience. To apply email letter of interest, resume and salary requirements to human_resources-at-tedpella.com. See our full job posting at www.tedpella.com Login Host: 12.7.209.242 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
The position at Delta College, Stockton, California, has been posted:
Associate Professor/Professor of Electron Microscopy
Salary: $51,912.00 - $106,386.00 Annually Location: Stockton, CA Job Type: Full Time Department: Applied Science, Business & Technology Job Number: 11-30-17 Closing: 12/8/2017 5:00 PM Pacific
Description Under the general supervision of the Division Dean of Applied Science, Business and Technology, the Associate Professor/Professor of Electron Microscopy teaches classes primarily in the electron microscopy discipline; the primary responsibility is to teach 15 units (per term) in the assigned discipline.
This is a one year temporary position, full time. Start date: January 2018
For more info follow this link:
https://www.governmentjobs.com/careers/deltacollege . It’s on page 4
NB: This is a one year position. The HR guys waited until the last minute and couldn’t get it together to do a full search for a permanent appointment. That is supposed to happen during next year. This closes in a week, so do not delay if you are interested. I can answer any questions via email.
Jon
Jonathan Krupp ASB&T San Joaquin Delta College 5151Pacific Ave. Stockton, CA 95207 (209) 954-5284 jkrupp-at-deltacollege.edu
After 12/30/17 my email will be: jkrupp267-at-gmail.com
==============================Original Headers============================== 13, 40 -- From prvs=1500be3cae=jkrupp-at-deltacollege.edu Thu Nov 30 15:19:19 2017 13, 40 -- Received: from mailin2.deltacollege.edu (mailin2.deltacollege.edu [207.62.175.116]) 13, 40 -- by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id vAULJJ3C014109 13, 40 -- for {Microscopy-at-microscopy.com} ; Thu, 30 Nov 2017 15:19:19 -0600 13, 40 -- Received: from mailin2.deltacollege.edu (localhost.localdomain [127.0.0.1]) 13, 40 -- by localhost (Email Security Appliance) with SMTP id 05C4217592E_A207533B 13, 40 -- for {Microscopy-at-microscopy.com} ; Thu, 30 Nov 2017 21:16:35 +0000 (GMT) 13, 40 -- Received: from zmail.deltacollege.edu (zmail.deltacampus.net [10.200.68.180]) 13, 40 -- (using TLSv1.2 with cipher ECDHE-RSA-AES256-GCM-SHA384 (256/256 bits)) 13, 40 -- (Client did not present a certificate) 13, 40 -- by mailin2.deltacollege.edu (Sophos Email Appliance) with ESMTPS id DE7F017374A_A207532F 13, 40 -- for {Microscopy-at-microscopy.com} ; Thu, 30 Nov 2017 21:16:34 +0000 (GMT) 13, 40 -- Received: from localhost (localhost [127.0.0.1]) 13, 40 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 2C5506D1E747 13, 40 -- for {Microscopy-at-microscopy.com} ; Thu, 30 Nov 2017 13:16:30 -0800 (PST) 13, 40 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 13, 40 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10032) 13, 40 -- with ESMTP id gBklVa7l53Pn for {Microscopy-at-microscopy.com} ; 13, 40 -- Thu, 30 Nov 2017 13:16:27 -0800 (PST) 13, 40 -- Received: from localhost (localhost [127.0.0.1]) 13, 40 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 919EA6D1E74F 13, 40 -- for {Microscopy-at-microscopy.com} ; Thu, 30 Nov 2017 13:16:25 -0800 (PST) 13, 40 -- X-Virus-Scanned: amavisd-new at zmail.deltacollege.edu 13, 40 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 13, 40 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10026) 13, 40 -- with ESMTP id AyNUbCKYkFq5 for {Microscopy-at-microscopy.com} ; 13, 40 -- Thu, 30 Nov 2017 13:16:25 -0800 (PST) 13, 40 -- Received: from [10.246.140.143] (unknown [10.246.140.143]) 13, 40 -- by zmail.deltacollege.edu (Postfix) with ESMTPSA id 53CC16D1E75C 13, 40 -- for {Microscopy-at-microscopy.com} ; Thu, 30 Nov 2017 13:16:18 -0800 (PST) 13, 40 -- From: Jonathan Krupp {jkrupp-at-deltacollege.edu} 13, 40 -- Content-Type: text/plain; charset=utf-8 13, 40 -- Subject: Instructor at Delta College 13, 40 -- Message-Id: {9C91EC88-B8E0-4F5F-8A5B-0840377D81F7-at-deltacollege.edu} 13, 40 -- Date: Thu, 30 Nov 2017 13:16:20 -0800 13, 40 -- To: Microscopy-at-microscopy.com 13, 40 -- Mime-Version: 1.0 (Mac OS X Mail 9.3 \(3124\)) 13, 40 -- X-Mailer: Apple Mail (2.3124) 13, 40 -- Content-Transfer-Encoding: 8bit 13, 40 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id vAULJJ3C014109 ==============================End of - Headers==============================
X-from: Elaine Humphrey {ech-at-uvic.ca} To: annie.muske-dukes-driggs-at-lonza.com {annie.muske-dukes-driggs-at-lonza.com}
Hi Annie and Henk Thank you so much for this question. I am always in need of good ideas.
We usually have a Solve the Mystery in the Family Affair, which is changing its name this year to Microscopic Explorations, for the delegates families and friends at the M&M conference.
The pattern has developed whereby in a session on the Wednesday afternoon, it typically starts with an introduction and then a lot of hands on activities using loupes and light microscopes adapted from the GEMS Microscopical Explorations activities. At least three of the stations have a “solve the mystery.” At the next meeting in Baltimore we are developing one step further and we hope to have Fold scopes and micro meteorites.
Then the participants go to the vendor hall where we usually have access to four sems to “Solve the Mystery”.
I have tried to correlate the mystery with the place and change it each year. For instance the CSI Albuquerque story was: The Governor of New Mexico is coming to town. He is a baseball fan and his favorite team is the Albuquerque Isotopes. He was going to have his photograph taken with the cup the Isotopes just won! But disaster struck! Someone stole the cup! It is very important we get the cup back. We have to find out who did it. The thief left several clues at the scene of the crime: Some pollen Some hair Some sand Some fibres
He/she wasn’t a very good thief!
There were the six suspects. We decided we could not use real people as suspects so we chose to go with Harry Potter villains Delores Umbridge When she was interviewed Delores Umbridge was wearing a red flower. She has brown straight hair; She had sandy shoes because she played golf and her golf ball went into a sandy bunker; She was dressed in pink clothes. Bellatrix Lestrange
When she was interviewed: Bellatrix Lestrange was wearing a pink flower; she has brown straight hair; she had sandy shoes because she was in the garden; she was wearing blue clothes. Draco Malfoy When he was interviewed Draco Malfoy was wearing a lily flower; he has blond straight hair; he had sandy shoes because he took a short cut through the sand box in the garden; he was wearing grey clothes. Voldemort
When he was interviewed: Voldemort was wearing a red flower; no hair; he had sandy shoes because he went hiking along a trail in California; he was wearing grey clothes. Lucius Malfoy
When he was interviewed: Lucius Malfoy was wearing a pink flower; he has blond straight hair; he had sandy shoes because he went hiking along a trail in California; he was wearing black clothes Severus Snape
When he was interviewed: Severus Snape was wearing a lily flower; he has straight grey hair; he had sandy shoes because he played golf and his golf ball went into a sandy bunker; he was wearing black clothes
Mostly the clues fit whatever materials we had, such as what three flowers with lots of pollen were available to us that day. Two suspects for each clue.
We didn’t want one clue to give the suspect away. It had to be two clues. Each group took on one type of material. On the stub there were three possibles and the actual to compare. So we had a flipchart at headquarters (the Outreach Booth) with the suspects down one side .and clues along the top. As each of the four groups got their answer they put a tick in the right box. It worked just fine.
In Portland we had access to an X-ray analysis sem. The story was:
Someone came to the desk in the Museum and handed an envelope over to the cashier. Written on the envelope was “ Put all the cash in the envelope and don’t say anything. I’ve got a gun!” She complied but followed him to see where he went. He took a bicycle and went through a construction site (sand), shaped the bike along a wall (metal for X-ray analysis), over a flower bed (pollen) which had thorny rose bushes (fibres). By that evening the police had four suspects each with sandy shoes and bike wheels, different materials making up the bikes etc etc.
In Nashville: Someone stole a very valuable Elvis guitar and put it up for sale clandestinely. One of the potential buyers wanted it authenticated by identifying the strings by X-ray analysis. I went to our local music store and asked if they had any strings from 1960s. They gave me one and I bought a new one. The composition is very different and easy to identify by X-ray analysis.
Now I need a story for Baltimore. I’ve never been to Baltimore. Is there something special we can use in a story? Best wishes
Elaine
Dr. Elaine C. Humphrey Advanced Microscopy Facility Bob Wright Science Centre A015 University of Victoria, Canada Lab: 250-853-3968 cell: 250-886-2068 website: http://www.stehm.uvic.ca
} Hi Annie, et al. } } I did a CSI type demo using light bulb filaments. The scenario was that } there was a traffic accident in the dark and each driver claimed the } others headlights were not on. The headlamps in both cars were broken. } The 2nd driver claims that the 1st driver turned on his headlights after } the bulbs were broken in order to cover his fault. The officer collected } the light bulb filaments from the cars and you are comparing them in the } SEM. (You can spin out the story as much as you want!) } } I took 2 small incandescent bulbs (15 watt if I remember), broke the } glass on one and then applied power so that it burned out. I took the } 2nd and broke the glass while it was running. I then mounted the 2 } filaments on an SEM sticky tab and put them in the scope. } } Note: be careful about breaking the glass. Do it safely! I wore } safety glasses and leather gloves and used a pair of channel pliers to } hold the bulb inside a mostly closed cardboard box to contain the glass } fragments. } } On examination, the student will find small spherical globs on one } filament and none on the other. Doing EDX shows that the spherical } globs are Si and O (i.e. glass that melted). The inference is that the } filament was hot when the glass was broken, hence the light bulb was on. } The other bulb is just oxidized and doesn't show any melted glass. } Hence it was not running when the glass broke and was burned out } afterwards. } } They seemed to enjoy the "real life" problem solving. } } Cheers, } Henk } } } } } -------------------- } } Hendrik O. Colijn } Center for Electron Microscopy and AnalysiS } The Ohio State University } 1305 Kinnear Rd, Suite 100, Columbus, OH 43212 } } colijn.1-at-osu.edu 614/643-3458 } cemas.osu.edu } } "Time is that quality of nature which keeps things from happening all at } once." (Ray Cummings - 1922) } Lately it doesn't seem to be working. } } ------ Original Message ------ } X-from: microscopy.listserver-at-gmail.com } To: colijn.1-at-osu.edu } Sent: 11/20/2017 8:38:57 PM } Subject: [Microscopy] viaWWW:EDS and SEM projector for Outreach } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America
Edgar Allan Poe theme? The Murders in the Rue Morgue? Probably not "the pit and the pendulum" or "the cask of amontillado"
Not sure what you could do with crab cakes! I believe that McCormick's has a large spice facility near the harbor front. We could smell it the last time we were there.
Cheers, Henk
Sent from my Verizon 4G LTE Smartphone
------ Original message------ *From: *microscopy.listserver-at-gmail.com *Date: *Thu, Nov 30, 2017 9:12 PM *To: *Colijn, Hendrik; *Cc: * *Subject:*[Microscopy] Re: viaWWW:EDS and SEM projector for Outreach
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
X-from: Elaine Humphrey {ech-at-uvic.ca} To: annie.muske-dukes-driggs-at-lonza.com {annie.muske-dukes-driggs-at-lonza.com}
Hi Annie and Henk Thank you so much for this question. I am always in need of good ideas.
We usually have a Solve the Mystery in the Family Affair, which is changing its name this year to Microscopic Explorations, for the delegates families and friends at the M&M conference.
The pattern has developed whereby in a session on the Wednesday afternoon, it typically starts with an introduction and then a lot of hands on activities using loupes and light microscopes adapted from the GEMS Microscopical Explorations activities. At least three of the stations have a solve the mystery. At the next meeting in Baltimore we are developing one step further and we hope to have Fold scopes and micro meteorites.
Then the participants go to the vendor hall where we usually have access to four sems to Solve the Mystery.
I have tried to correlate the mystery with the place and change it each year. For instance the CSI Albuquerque story was: The Governor of New Mexico is coming to town. He is a baseball fan and his favorite team is the Albuquerque Isotopes. He was going to have his photograph taken with the cup the Isotopes just won! But disaster struck! Someone stole the cup! It is very important we get the cup back. We have to find out who did it. The thief left several clues at the scene of the crime: Some pollen Some hair Some sand Some fibres
He/she wasnt a very good thief!
There were the six suspects. We decided we could not use real people as suspects so we chose to go with Harry Potter villains Delores Umbridge When she was interviewed Delores Umbridge was wearing a red flower. She has brown straight hair; She had sandy shoes because she played golf and her golf ball went into a sandy bunker; She was dressed in pink clothes. Bellatrix Lestrange
When she was interviewed: Bellatrix Lestrange was wearing a pink flower; she has brown straight hair; she had sandy shoes because she was in the garden; she was wearing blue clothes. Draco Malfoy When he was interviewed Draco Malfoy was wearing a lily flower; he has blond straight hair; he had sandy shoes because he took a short cut through the sand box in the garden; he was wearing grey clothes. Voldemort
When he was interviewed: Voldemort was wearing a red flower; no hair; he had sandy shoes because he went hiking along a trail in California; he was wearing grey clothes. Lucius Malfoy
When he was interviewed: Lucius Malfoy was wearing a pink flower; he has blond straight hair; he had sandy shoes because he went hiking along a trail in California; he was wearing black clothes Severus Snape
When he was interviewed: Severus Snape was wearing a lily flower; he has straight grey hair; he had sandy shoes because he played golf and his golf ball went into a sandy bunker; he was wearing black clothes
Mostly the clues fit whatever materials we had, such as what three flowers with lots of pollen were available to us that day. Two suspects for each clue.
We didnt want one clue to give the suspect away. It had to be two clues. Each group took on one type of material. On the stub there were three possibles and the actual to compare. So we had a flipchart at headquarters (the Outreach Booth) with the suspects down one side .and clues along the top. As each of the four groups got their answer they put a tick in the right box. It worked just fine.
In Portland we had access to an X-ray analysis sem. The story was:
Someone came to the desk in the Museum and handed an envelope over to the cashier. Written on the envelope was Put all the cash in the envelope and dont say anything. Ive got a gun! She complied but followed him to see where he went. He took a bicycle and went through a construction site (sand), shaped the bike along a wall (metal for X-ray analysis), over a flower bed (pollen) which had thorny rose bushes (fibres). By that evening the police had four suspects each with sandy shoes and bike wheels, different materials making up the bikes etc etc.
In Nashville: Someone stole a very valuable Elvis guitar and put it up for sale clandestinely. One of the potential buyers wanted it authenticated by identifying the strings by X-ray analysis. I went to our local music store and asked if they had any strings from 1960s. They gave me one and I bought a new one. The composition is very different and easy to identify by X-ray analysis.
Now I need a story for Baltimore. Ive never been to Baltimore. Is there something special we can use in a story? Best wishes
Elaine
Dr. Elaine C. Humphrey Advanced Microscopy Facility Bob Wright Science Centre A015 University of Victoria, Canada Lab: 250-853-3968 cell: 250-886-2068 website: http://www.stehm.uvic.ca
} Hi Annie, et al. } } I did a CSI type demo using light bulb filaments. The scenario was that } there was a traffic accident in the dark and each driver claimed the } others headlights were not on. The headlamps in both cars were broken. } The 2nd driver claims that the 1st driver turned on his headlights after } the bulbs were broken in order to cover his fault. The officer collected } the light bulb filaments from the cars and you are comparing them in the } SEM. (You can spin out the story as much as you want!) } } I took 2 small incandescent bulbs (15 watt if I remember), broke the } glass on one and then applied power so that it burned out. I took the } 2nd and broke the glass while it was running. I then mounted the 2 } filaments on an SEM sticky tab and put them in the scope. } } Note: be careful about breaking the glass. Do it safely! I wore } safety glasses and leather gloves and used a pair of channel pliers to } hold the bulb inside a mostly closed cardboard box to contain the glass } fragments. } } On examination, the student will find small spherical globs on one } filament and none on the other. Doing EDX shows that the spherical } globs are Si and O (i.e. glass that melted). The inference is that the } filament was hot when the glass was broken, hence the light bulb was on. } The other bulb is just oxidized and doesn't show any melted glass. } Hence it was not running when the glass broke and was burned out } afterwards. } } They seemed to enjoy the "real life" problem solving. } } Cheers, } Henk } } } } } -------------------- } } Hendrik O. Colijn } Center for Electron Microscopy and AnalysiS } The Ohio State University } 1305 Kinnear Rd, Suite 100, Columbus, OH 43212 } } colijn.1-at-osu.edu 614/643-3458 } cemas.osu.edu } } "Time is that quality of nature which keeps things from happening all at } once." (Ray Cummings - 1922) } Lately it doesn't seem to be working. } } ------ Original Message ------ } X-from: microscopy.listserver-at-gmail.com } To: colijn.1-at-osu.edu } Sent: 11/20/2017 8:38:57 PM } Subject: [Microscopy] viaWWW:EDS and SEM projector for Outreach } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America
Micheal Phelps, the American sports champion, was born in Baltimore. You can make some plans related to his medals!
A number of metallic medals with surface patterns can be designed, cut, and painted in yellow or silver or bronze colours, similar to those awarded in sports competitions.
The question can be "which medal belongs to Michael Phelps (if any) and which one is fake?"
Details can be designed in a way that participants need to do microscopy work and chemical analysis to find out the answer!
Best regards,
Nima, Queen's University
-------- Original Message --------
X-from: Elaine Humphrey {ech-at-uvic.ca} To: annie.muske-dukes-driggs-at-lonza.com {annie.muske-dukes-driggs-at-lonza.com}
Hi Annie and Henk Thank you so much for this question. I am always in need of good ideas.
We usually have a Solve the Mystery in the Family Affair, which is changing its name this year to Microscopic Explorations, for the delegates families and friends at the M&M conference.
The pattern has developed whereby in a session on the Wednesday afternoon, it typically starts with an introduction and then a lot of hands on activities using loupes and light microscopes adapted from the GEMS Microscopical Explorations activities. At least three of the stations have a solve the mystery. At the next meeting in Baltimore we are developing one step further and we hope to have Fold scopes and micro meteorites.
Then the participants go to the vendor hall where we usually have access to four sems to Solve the Mystery.
I have tried to correlate the mystery with the place and change it each year. For instance the CSI Albuquerque story was: The Governor of New Mexico is coming to town. He is a baseball fan and his favorite team is the Albuquerque Isotopes. He was going to have his photograph taken with the cup the Isotopes just won! But disaster struck! Someone stole the cup! It is very important we get the cup back. We have to find out who did it. The thief left several clues at the scene of the crime: Some pollen Some hair Some sand Some fibres
He/she wasnt a very good thief!
There were the six suspects. We decided we could not use real people as suspects so we chose to go with Harry Potter villains Delores Umbridge When she was interviewed Delores Umbridge was wearing a red flower. She has brown straight hair; She had sandy shoes because she played golf and her golf ball went into a sandy bunker; She was dressed in pink clothes. Bellatrix Lestrange
When she was interviewed: Bellatrix Lestrange was wearing a pink flower; she has brown straight hair; she had sandy shoes because she was in the garden; she was wearing blue clothes. Draco Malfoy When he was interviewed Draco Malfoy was wearing a lily flower; he has blond straight hair; he had sandy shoes because he took a short cut through the sand box in the garden; he was wearing grey clothes. Voldemort
When he was interviewed: Voldemort was wearing a red flower; no hair; he had sandy shoes because he went hiking along a trail in California; he was wearing grey clothes. Lucius Malfoy
When he was interviewed: Lucius Malfoy was wearing a pink flower; he has blond straight hair; he had sandy shoes because he went hiking along a trail in California; he was wearing black clothes Severus Snape
When he was interviewed: Severus Snape was wearing a lily flower; he has straight grey hair; he had sandy shoes because he played golf and his golf ball went into a sandy bunker; he was wearing black clothes
Mostly the clues fit whatever materials we had, such as what three flowers with lots of pollen were available to us that day. Two suspects for each clue.
We didnt want one clue to give the suspect away. It had to be two clues. Each group took on one type of material. On the stub there were three possibles and the actual to compare. So we had a flipchart at headquarters (the Outreach Booth) with the suspects down one side .and clues along the top. As each of the four groups got their answer they put a tick in the right box. It worked just fine.
In Portland we had access to an X-ray analysis sem. The story was:
Someone came to the desk in the Museum and handed an envelope over to the cashier. Written on the envelope was Put all the cash in the envelope and dont say anything. Ive got a gun! She complied but followed him to see where he went. He took a bicycle and went through a construction site (sand), shaped the bike along a wall (metal for X-ray analysis), over a flower bed (pollen) which had thorny rose bushes (fibres). By that evening the police had four suspects each with sandy shoes and bike wheels, different materials making up the bikes etc etc.
In Nashville: Someone stole a very valuable Elvis guitar and put it up for sale clandestinely. One of the potential buyers wanted it authenticated by identifying the strings by X-ray analysis. I went to our local music store and asked if they had any strings from 1960s. They gave me one and I bought a new one. The composition is very different and easy to identify by X-ray analysis.
Now I need a story for Baltimore. Ive never been to Baltimore. Is there something special we can use in a story? Best wishes
Elaine
Dr. Elaine C. Humphrey Advanced Microscopy Facility Bob Wright Science Centre A015 University of Victoria, Canada Lab: 250-853-3968 cell: 250-886-2068 website: https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.stehm.uvic.ca&data=02%7C01%7C14nnb1%40queensu.ca%7Cac6f78509dae425740e608d538640940%7Cd61ecb3b38b142d582c4efb2838b925c%7C1%7C0%7C636476924664745889&sdata=UWIs%2Fvo0E9DK4svQBPfEGop0Hshl1QbVrv9qy9usxoE%3D&reserved=0
} Hi Annie, et al. } } I did a CSI type demo using light bulb filaments. The scenario was that } there was a traffic accident in the dark and each driver claimed the } others headlights were not on. The headlamps in both cars were broken. } The 2nd driver claims that the 1st driver turned on his headlights after } the bulbs were broken in order to cover his fault. The officer collected } the light bulb filaments from the cars and you are comparing them in the } SEM. (You can spin out the story as much as you want!) } } I took 2 small incandescent bulbs (15 watt if I remember), broke the } glass on one and then applied power so that it burned out. I took the } 2nd and broke the glass while it was running. I then mounted the 2 } filaments on an SEM sticky tab and put them in the scope. } } Note: be careful about breaking the glass. Do it safely! I wore } safety glasses and leather gloves and used a pair of channel pliers to } hold the bulb inside a mostly closed cardboard box to contain the glass } fragments. } } On examination, the student will find small spherical globs on one } filament and none on the other. Doing EDX shows that the spherical } globs are Si and O (i.e. glass that melted). The inference is that the } filament was hot when the glass was broken, hence the light bulb was on. } The other bulb is just oxidized and doesn't show any melted glass. } Hence it was not running when the glass broke and was burned out } afterwards. } } They seemed to enjoy the "real life" problem solving. } } Cheers, } Henk } } } } } -------------------- } } Hendrik O. Colijn } Center for Electron Microscopy and AnalysiS } The Ohio State University } 1305 Kinnear Rd, Suite 100, Columbus, OH 43212 } } colijn.1-at-osu.edu 614/643-3458 } cemas.osu.edu } } "Time is that quality of nature which keeps things from happening all at } once." (Ray Cummings - 1922) } Lately it doesn't seem to be working. } } ------ Original Message ------ } X-from: microscopy.listserver-at-gmail.com } To: colijn.1-at-osu.edu } Sent: 11/20/2017 8:38:57 PM } Subject: [Microscopy] viaWWW:EDS and SEM projector for Outreach } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America
I've been helping a wildlife disease specialist at the USGS try to figure out what's wrong with a population of endangered crows that are dying off at an alarming rate. If you are interested and think you can offer an opinion, please look at the images on this server (the link is only good for one week):
These photomicrographs are from the lung of a critically endangered crow whose populations are declining probably because of pneumonia and hepatitis of unknown origin. On histology, crows manifest mild-to-marked non-suppurative interstitial pneumonia with varying degrees of vasculitis. No known organisms (protozoa, bacteria, fungi, viruses) are seen either on light or ultrastructural microscopy, and all laboratory tests for infectious agents are so far negative. On TEM, we are consistently seeing a similar pattern in lungs with filamentous tubular structures ca. 10-20 um diameter surrounding blood vessels. Might anyone have ideas of what these structure could be??
Aloha from rainy and gloomy Honolulu, Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 6, 20 -- From tina-at-pbrc.hawaii.edu Fri Dec 1 14:44:25 2017 6, 20 -- Received: from b1000.pbrc.hawaii.edu (b1000.pbrc.hawaii.edu [128.171.22.30]) 6, 20 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id vB1KiOxM013738 6, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 1 Dec 2017 14:44:25 -0600 6, 20 -- Received: from b1000.pbrc.hawaii.edu (localhost [127.0.0.1]) 6, 20 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id vB1Kfhq7028141 6, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 6, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 1 Dec 2017 10:41:43 -1000 (HST) 6, 20 -- Received: from localhost (tina-at-localhost) 6, 20 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP id vB1KfgiK028136 6, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 1 Dec 2017 10:41:43 -1000 (HST) 6, 20 -- X-Authentication-Warning: b1000.pbrc.hawaii.edu: tina owned process doing -bs 6, 20 -- Date: Fri, 1 Dec 2017 10:41:42 -1000 (HST) 6, 20 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 6, 20 -- X-X-Sender: tina-at-b1000 6, 20 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 6, 20 -- Subject: Need help from patho/histo types 6, 20 -- Message-ID: {Pine.GSO.4.64.1712011037310.28026-at-b1000} 6, 20 -- MIME-Version: 1.0 6, 20 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 4, 20 -- From tina-at-pbrc.hawaii.edu Fri Dec 1 15:15:04 2017 4, 20 -- Received: from b1000.pbrc.hawaii.edu (b1000.pbrc.hawaii.edu [128.171.22.30]) 4, 20 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id vB1LF3U1004659 4, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 1 Dec 2017 15:15:04 -0600 4, 20 -- Received: from b1000.pbrc.hawaii.edu (localhost [127.0.0.1]) 4, 20 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id vB1LCMPb028509 4, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 4, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 1 Dec 2017 11:12:22 -1000 (HST) 4, 20 -- Received: from localhost (tina-at-localhost) 4, 20 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP id vB1LCMQ8028505 4, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 1 Dec 2017 11:12:22 -1000 (HST) 4, 20 -- X-Authentication-Warning: b1000.pbrc.hawaii.edu: tina owned process doing -bs 4, 20 -- Date: Fri, 1 Dec 2017 11:12:22 -1000 (HST) 4, 20 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 4, 20 -- X-X-Sender: tina-at-b1000 4, 20 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 4, 20 -- Subject: Crow Lung link 4, 20 -- Message-ID: {Pine.GSO.4.64.1712011111020.28493-at-b1000} 4, 20 -- MIME-Version: 1.0 4, 20 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
Again, any opinions, musings, or speculations appreciated!
Aloha, Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 5, 20 -- From tina-at-pbrc.hawaii.edu Fri Dec 1 17:18:03 2017 5, 20 -- Received: from b1000.pbrc.hawaii.edu (b1000.pbrc.hawaii.edu [128.171.22.30]) 5, 20 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id vB1NI3Vd032393 5, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 1 Dec 2017 17:18:03 -0600 5, 20 -- Received: from b1000.pbrc.hawaii.edu (localhost [127.0.0.1]) 5, 20 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id vB1NFL5W029179 5, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 5, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 1 Dec 2017 13:15:22 -1000 (HST) 5, 20 -- Received: from localhost (tina-at-localhost) 5, 20 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP id vB1NFLTR029175 5, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 1 Dec 2017 13:15:21 -1000 (HST) 5, 20 -- X-Authentication-Warning: b1000.pbrc.hawaii.edu: tina owned process doing -bs 5, 20 -- Date: Fri, 1 Dec 2017 13:15:21 -1000 (HST) 5, 20 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 5, 20 -- X-X-Sender: tina-at-b1000 5, 20 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 5, 20 -- Subject: Another crow link 5, 20 -- Message-ID: {Pine.GSO.4.64.1712011313110.28493-at-b1000} 5, 20 -- MIME-Version: 1.0 5, 20 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
Unfortunately the McCormick warehouses moved up to the north side of the city many years ago. I miss the smells that were there by the Inner Harbor. There is also a railroad theme in that the first railroad station (B&O) is situated just west of Baltimore. There could be steel, coal, and slag involved. There is the Johns Hopkins Hospital with a plethora of medical samples (blood?). The Chesapeake Bay has lots of biota. There is the infamous Chromium smelting talus that the Inner Harbor is partially built on (maybe you don’t want to have samples of Hexavalent Chromium around). Also, the Babe Ruth Museum for a baseball theme (including the Orioles). The Poe theme can have both raven’s feather and Ravens leather. To add a twist to that John Astin (Gomez Addams from the Addam Family) was born here and teaches at Johns Hopkins.
Then there is history of Frederick Douglas and the Underground Railroad.
Fort McHenry where the Star Spangled Banner was penned by Francis Scott Key and the flag sewn by Mary Pickersgill in 1813 that resides in the Smithsonian. There are cloth fibers (red and blue wool and white cotton) and gun powder themes here.
Enoch Pratt Free Library — one of the oldest libraries in the US, Peabody Library focused on music (paper, vinyl, tape).
There’s much more but I have to do some work today.
Ken
Kenneth JT Livi, PhD Director, Materials Characterization and Processing Center Materials Science and Engineering 3400 N Charles Street Johns Hopkins University Baltimore, Maryland 21218 USA
} On Dec 1, 2017, at 7:55 AM, microscopy.listserver-at-gmail.com wrote: } } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: Colijn, Hendrik {colijn.1-at-osu.edu} } } } } Edgar Allan Poe theme? } The Murders in the Rue Morgue? } Probably not "the pit and the pendulum" or "the cask of amontillado" } } Not sure what you could do with crab cakes! I believe that McCormick's has a large spice facility } near the harbor front. We could smell it the last time we were there. } } Cheers, } Henk } } Sent from my Verizon 4G LTE Smartphone } } ------ Original message------ } *From: *microscopy.listserver-at-gmail.com } *Date: *Thu, Nov 30, 2017 9:12 PM } *To: *Colijn, Hendrik; } *Cc: * } *Subject:*[Microscopy] Re: viaWWW:EDS and SEM projector for Outreach } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: Elaine Humphrey {ech-at-uvic.ca} } To: annie.muske-dukes-driggs-at-lonza.com {annie.muske-dukes-driggs-at-lonza.com} } } Hi Annie and Henk } Thank you so much for this question. I am always in need of good ideas. } } We usually have a Solve the Mystery in the Family Affair, which is changing its name this year to } Microscopic Explorations, for the delegates families and friends at the M&M conference. } } The pattern has developed whereby in a session on the Wednesday afternoon, it typically starts with } an introduction and then a lot of hands on activities using loupes and light microscopes adapted } from the GEMS Microscopical Explorations activities. At least three of the stations have a solve } the mystery. At the next meeting in Baltimore we are developing one step further and we hope to } have Fold scopes and micro meteorites. } } Then the participants go to the vendor hall where we usually have access to four sems to Solve the } Mystery. } } I have tried to correlate the mystery with the place and change it each year. For instance the CSI } Albuquerque story was: The Governor of New Mexico is coming to town. He is a baseball fan and his } favorite team is the Albuquerque Isotopes. } He was going to have his photograph taken with the cup the Isotopes just won! But disaster struck! } Someone stole the cup! It is very important we get the cup back. We have to find out who did it. } The thief left several clues at the scene of the crime: } Some pollen } Some hair } Some sand } Some fibres } } He/she wasnt a very good thief! } } } } There were the six suspects. We decided we could not use real people as suspects so we chose to go } with Harry Potter villains } Delores Umbridge } When she was interviewed Delores Umbridge was wearing a red flower. She has brown straight hair; She } had sandy shoes because she played golf and her golf ball went into a sandy bunker; She was dressed } in pink clothes. } Bellatrix Lestrange } } When she was interviewed: Bellatrix Lestrange was wearing a pink flower; she has brown straight } hair; she had sandy shoes because she was in the garden; she was wearing blue clothes. } Draco Malfoy } When he was interviewed Draco Malfoy was wearing a lily flower; he has blond straight hair; he had } sandy shoes because he took a short cut through the sand box in the garden; he was wearing grey clothes. } Voldemort } } When he was interviewed: Voldemort was wearing a red flower; no hair; he had sandy shoes because he } went hiking along a trail in California; he was wearing grey clothes. } Lucius Malfoy } } When he was interviewed: Lucius Malfoy was wearing a pink flower; he has blond straight hair; he had } sandy shoes because he went hiking along a trail in California; he was wearing black clothes } Severus Snape } } When he was interviewed: Severus Snape was wearing a lily flower; he has straight grey hair; he } had sandy shoes because he played golf and his golf ball went into a sandy bunker; he was wearing } black clothes } } } } Mostly the clues fit whatever materials we had, such as what three flowers with lots of pollen were } available to us that day. Two suspects for each clue. } } } We didnt want one clue to give the suspect away. It had to be two clues. Each group took on one } type of material. On the stub there were three possibles and the actual to compare. So we had a } flipchart at headquarters (the Outreach Booth) with the suspects down one side .and clues along the } top. As each of the four groups got their answer they put a tick in the right box. It worked just fine. } } In Portland we had access to an X-ray analysis sem. The story was: } } Someone came to the desk in the Museum and handed an envelope over to the cashier. Written on the } envelope was Put all the cash in the envelope and dont say anything. Ive got a gun! } She complied but followed him to see where he went. He took a bicycle and went through a } construction site (sand), shaped the bike along a wall (metal for X-ray analysis), over a flower bed } (pollen) which had thorny rose bushes (fibres). By that evening the police had four suspects each } with sandy shoes and bike wheels, different materials making up the bikes etc etc. } } In Nashville: Someone stole a very valuable Elvis guitar and put it up for sale clandestinely. One } of the potential buyers wanted it authenticated by identifying the strings by X-ray analysis. I went } to our local music store and asked if they had any strings from 1960s. They gave me one and I bought } a new one. The composition is very different and easy to identify by X-ray analysis. } } Now I need a story for Baltimore. Ive never been to Baltimore. Is there something special we can } use in a story? } Best wishes } } Elaine } } Dr. Elaine C. Humphrey } Advanced Microscopy Facility } Bob Wright Science Centre A015 } University of Victoria, Canada } Lab: 250-853-3968 } cell: 250-886-2068 } website: http://www.stehm.uvic.ca } } } } } } } } } } } Hi Annie, et al. } } } } I did a CSI type demo using light bulb filaments. The scenario was that } } there was a traffic accident in the dark and each driver claimed the } } others headlights were not on. The headlamps in both cars were broken. } } The 2nd driver claims that the 1st driver turned on his headlights after } } the bulbs were broken in order to cover his fault. The officer collected } } the light bulb filaments from the cars and you are comparing them in the } } SEM. (You can spin out the story as much as you want!) } } } } I took 2 small incandescent bulbs (15 watt if I remember), broke the } } glass on one and then applied power so that it burned out. I took the } } 2nd and broke the glass while it was running. I then mounted the 2 } } filaments on an SEM sticky tab and put them in the scope. } } } } Note: be careful about breaking the glass. Do it safely! I wore } } safety glasses and leather gloves and used a pair of channel pliers to } } hold the bulb inside a mostly closed cardboard box to contain the glass } } fragments. } } } } On examination, the student will find small spherical globs on one } } filament and none on the other. Doing EDX shows that the spherical } } globs are Si and O (i.e. glass that melted). The inference is that the } } filament was hot when the glass was broken, hence the light bulb was on. } } The other bulb is just oxidized and doesn't show any melted glass. } } Hence it was not running when the glass broke and was burned out } } afterwards. } } } } They seemed to enjoy the "real life" problem solving. } } } } Cheers, } } Henk } } } } } } } } } } -------------------- } } } } Hendrik O. Colijn } } Center for Electron Microscopy and AnalysiS } } The Ohio State University } } 1305 Kinnear Rd, Suite 100, Columbus, OH 43212 } } } } colijn.1-at-osu.edu 614/643-3458 } } cemas.osu.edu } } } } "Time is that quality of nature which keeps things from happening all at } } once." (Ray Cummings - 1922) } } Lately it doesn't seem to be working. } } } } ------ Original Message ------ } } X-from: microscopy.listserver-at-gmail.com } } To: colijn.1-at-osu.edu } } Sent: 11/20/2017 8:38:57 PM } } Subject: [Microscopy] viaWWW:EDS and SEM projector for Outreach } } } } } } } } } } } } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } } America } } } To Subscribe/Unsubscribe -- } } } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } } } X-from: Greg Baty {gbaty-at-pdx.edu} } } } } } } } } } Annie, } } } } } } I usually deal with kids in the 12 - 18 YO range for outreach.ย In the } } } past we offered a week long } } } half day experience in the lab.ย In my experience it is critical to } } } find samples that kids can } } } relate to. } } } } } } Insects can be a good for imaging.ย My favorites are honey bees, house } } } fly's and fruit fly's.ย Kids } } } really like the structure of the eye. } } } } } } Epsom salt has a nice structure and is useful for EDX; however, it can } } } take a long time to get } } } vacuum since it is usually hydrated.ย Best to only use a few crystals } } } or prepump. } } } } } } Geological samples.ย I prefer a thin section to reduce pump time, but } } } bulk sections work too.ย If } } } you need a thin section I suspect Spectrum Petrographics could help. I } } } have no financial connection } } } to them, but I know several of our geology Professors have Spectrum do } } } their mounts. } } } } } } I had one student bring hair and feathers from the farm she lives on.ย } } } There is a significant } } } difference between animals. } } } } } } House hold dust can be a good sample.ย Just pick a spot like a window } } } sill and don't clean it for a } } } week or two.ย Collect the sample with C tape on a stub.ย I have seen } } } pollen, insect parts and } } } silicate minerals. } } } } } } Greg } } } } } } On Fri, Nov 17, 2017 at 3:49 PM, {microscopy.listserver-at-gmail.com } } } {mailto:microscopy.listserver-at-gmail.com} } wrote: } } } } } } } } } } } } } } } } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor:ย The Microscopy Society of } } } America } } } Toย Subscribe/Unsubscribe -- } } } http://www.microscopy.com/MicroscopyListserver } } } On-Line {http://www.microscopy.com/MicroscopyListserver On-Line} } } } Help } } } http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } {http://www.microscopy.com/MicroscopyListserver/FAQ.html} } } } } } } ---------------------------------------------------------------------------- } } } } } } X-from: annie.muske-dukes-driggs-at-lonza.com } } } {mailto:annie.muske-dukes-driggs-at-lonza.com} } } } } } } This Question/Comment was submitted to the Microscopy Listserver } } } using the WWW based Form at } } } http://www.microscopy.com/MLFormMail.html } } } {http://www.microscopy.com/MLFormMail.html} } } } } } } --------------------------------------------------------------------------- } } } Remember this posting is most likely not from a Subscriber, so when } } } replying pleaseย copyย both annie.muske-dukes-driggs-at-lonza.com } } } {mailto:annie.muske-dukes-driggs-at-lonza.com} as well } } } asย ย the Microscopy Listserver } } } } } } --------------------------------------------------------------------------- } } } } } } Email: annie.muske-dukes-driggs-at-lonza.com } } } {mailto:annie.muske-dukes-driggs-at-lonza.com} Name: } } } Annie Muske-Dukes } } } } } } Organization: Bend Research } } } } } } Title-Subject: [Filtered] EDS and SEM projector for Outreach } } } } } } Message: Hi everyone, } } } } } } My company runs a community outreach program for local elementary } } } school } } } students (8-10 years old). They tour our facility and see quick } } } demonstrations/lectures on a variety of topics. } } } We have had an SEM demo for years and I would like to incorporate } } } our } } } EDS but I'm having trouble coming up with samples that kids that } } } age } } } understand, especially since I only have 15 minutes per group and } } } we try } } } to go through at least four samples. } } } } } } I have generated a map of different elements in a vitamin tablet } } } which } } } went over well and gives them a good understanding of what the } } } system is } } } doing. Any recommendations for samples that generate interesting } } } maps or } } } have unexpected elements? I'll be doing the prep/high quality maps } } } ahead } } } of time so that doesn't need to be done in the 15 minutes. } } } } } } I'd also like to get a wireless projector to use with our Hitachi } } } SU3500 } } } to project a live image of the screen onto the wall. Our service } } } rep } } } said some models can cause driver issues so if anyone here has a } } } model } } } that works please let me know. We need to stay under US$500, but } } } under } } } $300 would be ideal. } } } } } } Thanks, } } } Annie (I am a list subscriber but our required corporate } } } attachments } } } prevent me from sending the usual way) } } } } } } ย Login Host: 64.47.109.63 } } } ย Listserver Email Form V - 20120416 } } } } } } --------------------------------------------------------------------------- } } } } } } } } } } } } ==============================Original } } } Headers============================== } } } 13, 53 -- From microscopy.listserver-at-gmail.com } } } {mailto:microscopy.listserver-at-gmail.com} Fri Nov } } } 17 17:18:56 2017 } } } 13, 53 -- Received: from mail-pf0-f175.google.com } } } {http://mail-pf0-f175.google.com} } } } (mail-pf0-f175.google.com {http://mail-pf0-f175.google.com} } } } [209.85.192.175]) } } } 13, 53 --ย ย ย ย by microscopy.com {http://microscopy.com} } } } (8.12.11.20060308/8.12.8) with ESMTP } } } id vAHNItoN009038 } } } 13, 53 --ย ย ย ย for {microscopy-at-microscopy.com } } } {mailto:microscopy-at-microscopy.com} } ; Fri, 17 Nov } } } 2017 17:18:55 -0600 } } } 13, 53 -- Received: by mail-pf0-f175.google.com } } } {http://mail-pf0-f175.google.com} with SMTP id } } } x7so2994822pfa.1 } } } 13, 53 --ย ย ย ย ย for {microscopy-at-microscopy.com } } } {mailto:microscopy-at-microscopy.com} } ; Fri, 17 } } } Nov 2017 15:15:30 -0800 (PST) } } } 13, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } } } 13, 53 --ย ย ย ย ย d=gmail.com {http://gmail.com} ; s=20161025; } } } 13, 53 --ย ย ย ย ย } } } h=subject:references:to:from:message-id:date:user-agent:mime-version } } } 13, 53 --ย ย ย ย ย } } } :in-reply-to:content-language:content-transfer-encoding; } } } 13, 53 --ย ย ย ย ย } } } bh=ajHXaowS7Xr4ulq5L7g6cSgXpHzLsIrp0ixP0W269Yg=; } } } 13, 53 --ย ย ย ย ย } } } b=eAW4Xc2CZ5KfsTKhl5W7M/p2Y/WXg6iJXwJQo9hPbmsciy3Vck/Ax2o7BirKvnpdNX } } } 13, 53 --ย ย ย ย ย } } } OHTQaVaboEyxaXc7fXVUBMmkkmN+iV6G180P48hIK3TBBvUpZT2G4LwsRl/uSiTcn+nz } } } 13, 53 --ย ย ย ย ย } } } x5fEA9ygmixMEaa6gk6e/kYBbOHHJY91XyIfC5mpUS8mYQd9W03hYSd9j4792grF6hYh } } } 13, 53 --ย ย ย ย ย } } } Y6iq/B2scCSA1R0hxix7wW4L+fJUpFRTNthPfi3uuMdf9+Nmi1yAEC+3Pi9kr69xnL4U } } } 13, 53 --ย ย ย ย ย } } } 8rmapnMno5eW0z7bA3tUYDUxmCzw9TSppsLLY02yiL+Qzvt+7X6TZQGvW3MeDZynUCL4 } } } 13, 53 --ย ย ย ย ย Jd5Q== } } } 13, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; } } } c=relaxed/relaxed; } } } 13, 53 --ย ย ย ย ย d=1e100.net {http://1e100.net} ; s=20161025; } } } 13, 53 --ย ย ย ย ย } } } h=x-gm-message-state:subject:references:to:from:message-id:date } } } 13, 53 --ย ย ย ย ย } } } :user-agent:mime-version:in-reply-to:content-language } } } 13, 53 --ย ย ย ย ย :content-transfer-encoding; } } } 13, 53 --ย ย ย ย ย } } } bh=ajHXaowS7Xr4ulq5L7g6cSgXpHzLsIrp0ixP0W269Yg=; } } } 13, 53 --ย ย ย ย ย } } } b=UE5U+qloOyYI02cv3X62Aen7ygRVtpC9be1euW6ToL9RWOUNDzJuYicaiYHteGM+Qd } } } 13, 53 --ย ย ย ย ย } } } qtJ39xxJ5fCRtWOA/2craX4llZDjvyEvBu8qvgFEu0rF1s6Iq6mVP22f81Ue/sr9d/IR } } } 13, 53 --ย ย ย ย ย } } } 6fP5utPLZ77wf8zE03W8OF7aAh6jcs2CqcLdDOKut29d29W/tXo2uc/6fvFGZfrm0KC4 } } } 13, 53 --ย ย ย ย ย } } } Y8escGfPPpC2PjflO0YYjziQs6DRvEKHz2P6szOnnILH98LcBZW5PuiKEXBwpNSTgFMj } } } 13, 53 --ย ย ย ย ย } } } Fq8fQCSelKePgB2xQ4HTqJJWfkTSxsRX+YTD+e8VukuWKoI90w/IYGW2+8/ep8UuppN7 } } } 13, 53 --ย ย ย ย ย bMQA== } } } 13, 53 -- X-Gm-Message-State: } } } AJaThX6HbDIzxLHVVc7CeFhyQ7NEofsBNhRwjrnchA1onNOMwoQ/xU4k } } } 13, 53 --ย ย ย ย GlNT9VefdH3phrwGEcxoF+YUtwv8 } } } 13, 53 -- X-Google-Smtp-Source: } } } } } } AGs4zMa17Mah5ciiqmYbjFsgBJIMp4Q9FQSWDIx5LCnxz99Kp3+HdN2/YzJUDJMfrLF91MumAUhpXQ== } } } 13, 53 -- X-Received: by 10.84.193.129 with SMTP id } } } f1mr6807886pld.317.1510960529479; } } } 13, 53 --ย ย ย ย ย Fri, 17 Nov 2017 15:15:29 -0800 (PST) } } } 13, 53 -- Received: from } } } Nestors-MacBookAir-Pro-2014-ElCapitan-OSX-1163.local ([182.16.234.14]) } } } 13, 53 --ย ย ย ย ย by smtp.googlemail.com } } } {http://smtp.googlemail.com} with ESMTPSA id } } } p19sm9352707pfj.140.2017.11.17.15.15.24 } } } 13, 53 --ย ย ย ย ย for {microscopy-at-microscopy.com } } } {mailto:microscopy-at-microscopy.com} } } } } 13, 53 --ย ย ย ย ย (version=TLS1_2 } } } cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } } } 13, 53 --ย ย ย ย ย Fri, 17 Nov 2017 15:15:24 -0800 (PST) } } } 13, 53 -- Subject: viaWWW:EDS and SEM projector for Outreach } } } 13, 53 -- References: {201711162229.vAGMTWp6026431-at-microscopy.com } } } {mailto:201711162229.vAGMTWp6026431-at-microscopy.com} } } } } 13, 53 -- To: MicroscopyListServer-Forward } } } {microscopy-at-microscopy.com } } } {mailto:microscopy-at-microscopy.com} } } } } 13, 53 -- From: Microscopy Listserver } } } {microscopy.listserver-at-gmail.com } } } {mailto:microscopy.listserver-at-gmail.com} } } } } 13, 53 -- X-Forwarded-Message-Id: } } } {201711162229.vAGMTWp6026431-at-microscopy.com } } } {mailto:201711162229.vAGMTWp6026431-at-microscopy.com} } } } } 13, 53 -- Message-ID: } } } {4230175d-0ecf-bad3-c4f0-4f12f99a8f95-at-gmail.com } } } {mailto:4230175d-0ecf-bad3-c4f0-4f12f99a8f95-at-gmail.com} } } } } 13, 53 -- Date: Sat, 18 Nov 2017 07:15:22 +0800 } } } 13, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; } } } rv:52.0) } } } 13, 53 --ย Gecko/20100101 Thunderbird/52.4.0 } } } 13, 53 -- MIME-Version: 1.0 } } } 13, 53 -- In-Reply-To: {201711162229.vAGMTWp6026431-at-microscopy.com } } } {mailto:201711162229.vAGMTWp6026431-at-microscopy.com} } } } } 13, 53 -- Content-Type: text/plain; charset=windows-1252; } } } format=flowed } } } 13, 53 -- Content-Language: en-US } } } 13, 53 -- Content-Transfer-Encoding: 7bit } } } ==============================End of - } } } Headers============================== } } } } } } } } } } } } } } } -- } } } Greg Baty } } } Center for Electron Microscopy and Nanofabrication } } } Portland State University } } } 503-725-2867 } } } www.pdx.edu/cemn {http://www.pdx.edu/cemn} {http://www.pdx.edu/cemn} } } } } } } ==============================Original } } } Headers============================== } } } 33, 53 -- From microscopy.listserver-at-gmail.com Mon Nov 20 19:36:44 2017 } } } 33, 53 -- Received: from mail-it0-f44.google.com } } } (mail-it0-f44.google.com [209.85.214.44]) } } } 33, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } } } vAL1aix6015707 } } } 33, 53 -- for {microscopy-at-microscopy.com} ; Mon, 20 Nov 2017 19:36:44 } } } -0600 } } } 33, 53 -- Received: by mail-it0-f44.google.com with SMTP id } } } m191so94474itg.2 } } } 33, 53 -- for {microscopy-at-microscopy.com} ; Mon, 20 Nov 2017 } } } 17:33:29 -0800 (PST) } } } 33, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } } } 33, 53 -- d=gmail.com; s=20161025; } } } 33, 53 -- } } } h=subject:references:to:from:message-id:date:user-agent:mime-version } } } 33, 53 -- } } } :in-reply-to:content-language:content-transfer-encoding; } } } 33, 53 -- bh=/F/T7b2ateC2XsLEfi9RkPQP3HXTOGoFcz53PnVgcoI=; } } } 33, 53 -- } } } b=OUO/SyStT9xPZ4r89GrT6EjUPBuRFC9l8vd8xkOc1Wayxalbb9C+mqPP8/NWgzg4dr } } } 33, 53 -- } } } OFqE4alBS0KMSSZJWmfohnFbhtSIysENTeR0FE7ee0HlBbRx8g3OtefAKNBRtvFIuOLo } } } 33, 53 -- } } } /LEFzS+mh07DGmii2Nt4o4wD7ne032P5IgmULSN20Q3n5Lovj1MV6YxhrnND8UYy7a7W } } } 33, 53 -- } } } 7otkQyD2rFUKvJbM+25NDXNpqX5Ed3I0q/Iqe4T4WHOILvz9t+gaK8WJcEEMalcez8Uv } } } 33, 53 -- } } } 7s6JnCML7lc2Q460Kw0ERmhJxcMA2WVkOlh6oTL7c0w3uxgFYqWLiXmK0Nj2yePWqpq2 } } } 33, 53 -- 1OoQ== } } } 33, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; } } } c=relaxed/relaxed; } } } 33, 53 -- d=1e100.net; s=20161025; } } } 33, 53 -- } } } h=x-gm-message-state:subject:references:to:from:message-id:date } } } 33, 53 -- } } } :user-agent:mime-version:in-reply-to:content-language } } } 33, 53 -- :content-transfer-encoding; } } } 33, 53 -- bh=/F/T7b2ateC2XsLEfi9RkPQP3HXTOGoFcz53PnVgcoI=; } } } 33, 53 -- } } } b=OWSg9XzDy3+XzUaRg0F2VSbucdrW8e7H0aNa/LfSDSy6Lji8rYEwlhaTkyAmYxyQUv } } } 33, 53 -- } } } t9x4ytLuAQE0LM4zJ9up24/aP6n9NQXyVFuaBV1VFQsuyuNSS+rM63qSYTBUm01lbOUZ } } } 33, 53 -- } } } BfCvXhOorCKmqW88mAREkaAni+B/Bmzib/EU1iJ0p4h+byUgHZTBV2gESUKNUnJmZXEc } } } 33, 53 -- } } } DOY9s/4V8S+0KRIEhnFMvFNg1fzgs38kW+mxZPfJQuVLGCqhvm35HDopnwUYUJABHAF6 } } } 33, 53 -- } } } /8lDAfgbFMXibj91tzreRatHG4nnRL+JsC4vHNp6CLzWFixF/rcWJrKKytOpX3Q5j425 } } } 33, 53 -- ZiyA== } } } 33, 53 -- X-Gm-Message-State: } } } AJaThX7iBJBYRYoJTq5wwwtvFSYsD7iJMYdUhjmpliq/ss7rDLBowdE7 } } } 33, 53 -- 530duzpT5Kmv1fRzwmKDnbkMmt+h } } } 33, 53 -- X-Google-Smtp-Source: } } } AGs4zMb2N1wqKXFXLro1KFdZ3viGm6+WXwfo6jNXY9+C4sI9x1sJRj3kGV2cdvLHk/pj0f5pDIeFaQ== } } } 33, 53 -- X-Received: by 10.36.9.146 with SMTP id } } } 140mr21499245itm.54.1511228008678; } } } 33, 53 -- Mon, 20 Nov 2017 17:33:28 -0800 (PST) } } } 33, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net } } } ([2603:300a:f04:7100:c1ce:57a0:e8a:603e]) } } } 33, 53 -- by smtp.googlemail.com with ESMTPSA id } } } e203sm23529itb.32.2017.11.20.17.33.28 } } } 33, 53 -- for {microscopy-at-microscopy.com} } } } 33, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 } } } bits=128/128); } } } 33, 53 -- Mon, 20 Nov 2017 17:33:28 -0800 (PST) } } } 33, 53 -- Subject: viaWWW:EDS and SEM projector for Outreach } } } 33, 53 -- References: } } } {CAEw=MCG18nxj3Qta+5PRcScAHuM5OXTuwrPkMo=iRUfziAeVAA-at-mail.gmail.com} } } } 33, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } } } 33, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } } } 33, 53 -- X-Forwarded-Message-Id: } } } {CAEw=MCG18nxj3Qta+5PRcScAHuM5OXTuwrPkMo=iRUfziAeVAA-at-mail.gmail.com} } } } 33, 53 -- Message-ID: {eaf57f96-804f-e18c-b045-52f84b411735-at-gmail.com} } } } 33, 53 -- Date: Mon, 20 Nov 2017 19:33:27 -0600 } } } 33, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; } } } rv:52.0) } } } 33, 53 -- Gecko/20100101 Thunderbird/52.4.0 } } } 33, 53 -- MIME-Version: 1.0 } } } 33, 53 -- In-Reply-To: } } } {CAEw=MCG18nxj3Qta+5PRcScAHuM5OXTuwrPkMo=iRUfziAeVAA-at-mail.gmail.com} } } } 33, 53 -- Content-Type: text/plain; charset=utf-8; format=flowed } } } 33, 53 -- Content-Language: en-US } } } 33, 53 -- Content-Transfer-Encoding: 8bit } } } ==============================End of - } } } Headers============================== } } } } } } } } ==============================Original Headers============================== } } 18, 125 -- From colijn.1-at-osu.edu Tue Nov 21 07:44:53 2017 } } 18, 125 -- Received: from NAM03-BY2-obe.outbound.protection.outlook.com (mail-by2nam03on0105.outbound.protection.outlook.com [104.47.42.105]) } } 18, 125 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id vALDiqTJ011892 } } 18, 125 -- for {microscopy-at-microscopy.com} ; Tue, 21 Nov 2017 07:44:52 -0600 } } 18, 125 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=osu.edu; s=selector1; } } 18, 125 -- h=From:Date:Subject:Message-ID:Content-Type:MIME-Version; } } 18, 125 -- bh=3WsifJvqT6wI5Pu9/VzI8h1WplGD0FvIV60sJNucWtk=; } } 18, 125 -- b=KYRk1AVBhtQ17yHz9NT9edF/qobbjxnrToXpplk1Q6YROWqi9IkEVvSLqhjGJAgGgoN7ybS2yCMhB2qAPFQ7BKSV8OlyH9d4w1fyAUCAkZ1KRFeZbhTfEhb/n1622Q0YZ1Rz4QEYsSpVK6X9xegX9Esga9dBDYKwTM1n1jBSVIU= } } 18, 125 -- Received: from DM5PR0101CA0027.prod.exchangelabs.com (2603:10b6:4:28::40) by } } 18, 125 -- CO1PR01MB304.prod.exchangelabs.com (2a01:111:e400:1026::26) with Microsoft } } 18, 125 -- SMTP Server (version=TLS1_2, } } 18, 125 -- cipher=TLS_ECDHE_RSA_WITH_AES_256_CBC_SHA384_P256) id 15.20.218.12; Tue, 21 } } 18, 125 -- Nov 2017 13:41:36 +0000 } } 18, 125 -- Received: from DM3NAM05FT053.eop-nam05.prod.protection.outlook.com } } 18, 125 -- (2a01:111:f400:7e51::206) by DM5PR0101CA0027.outlook.office365.com } } 18, 125 -- (2603:10b6:4:28::40) with Microsoft SMTP Server (version=TLS1_2, } } 18, 125 -- cipher=TLS_ECDHE_RSA_WITH_AES_256_CBC_SHA384) id 15.20.239.5 via Frontend } } 18, 125 -- Transport; Tue, 21 Nov 2017 13:41:36 +0000 } } 18, 125 -- Authentication-Results: spf=pass (sender IP is 128.146.138.9) } } 18, 125 -- smtp.mailfrom=osu.edu; microscopy.com; dkim=none (message not signed) } } 18, 125 -- header.d=none;microscopy.com; dmarc=pass action=none header.from=osu.edu; } } 18, 125 -- Received-SPF: Pass (protection.outlook.com: domain of osu.edu designates } } 18, 125 -- 128.146.138.9 as permitted sender) receiver=protection.outlook.com; } } 18, 125 -- client-ip=128.146.138.9; helo=cio-socc-esr03.osuad.osu.edu; } } 18, 125 -- Received: from cio-socc-esr03.osuad.osu.edu (128.146.138.9) by } } 18, 125 -- DM3NAM05FT053.mail.protection.outlook.com (10.152.98.167) with Microsoft SMTP } } 18, 125 -- Server (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_256_CBC_SHA384_P256) id } } 18, 125 -- 15.20.239.4 via Frontend Transport; Tue, 21 Nov 2017 13:41:36 +0000 } } 18, 125 -- Received: from CIO-TNC-HT06.osuad.osu.edu (localhost [127.0.0.1]) } } 18, 125 -- (using TLSv1.2 with cipher AES256-SHA256 (256/256 bits)) } } 18, 125 -- (No client certificate requested) } } 18, 125 -- by cio-socc-esr03.osuad.osu.edu (Postfix) with ESMTPS id 93E5FA0 } } 18, 125 -- for {microscopy-at-microscopy.com} ; Tue, 21 Nov 2017 08:41:34 -0500 (EST) } } 18, 125 -- Received: from [192.168.10.60] (199.18.22.26) by smtp.service.osu.edu } } 18, 125 -- (164.107.81.254) with Microsoft SMTP Server (TLS) id 14.3.319.2; Tue, 21 Nov } } 18, 125 -- 2017 08:41:34 -0500 } } 18, 125 -- From: Henk Colijn {colijn.1-at-osu.edu} } } 18, 125 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} } } 18, 125 -- Subject: Re: [Microscopy] viaWWW:EDS and SEM projector for Outreach } } 18, 125 -- Date: Tue, 21 Nov 2017 03:04:53 +0000 } } 18, 125 -- Message-ID: {em4ab7558a-a8b1-4069-adf0-d64fad009cee-at-hoc-asus} } } 18, 125 -- In-Reply-To: {201711210138.vAL1cvN6018239-at-microscopy.com} } } 18, 125 -- References: {201711210138.vAL1cvN6018239-at-microscopy.com} } } 18, 125 -- Reply-To: Henk Colijn {colijn.1-at-osu.edu} } } 18, 125 -- User-Agent: eM_Client/7.1.30794.0 } } 18, 125 -- MIME-Version: 1.0 } } 18, 125 -- Content-Type: text/plain; format=flowed; charset="utf-8" } } 18, 125 -- X-Originating-IP: [199.18.22.26] } } 18, 125 -- x-header-sapphire: true } } 18, 125 -- X-CFilter-Loop: Reflected } } 18, 125 -- X-EOPAttributedMessage: 0 } } 18, 125 -- X-Forefront-Antispam-Report: } } 18, 125 -- CIP:128.146.138.9;IPV:NLI;CTRY:US;EFV:NLI;SFV:NSPM;SFS:(10019020)(6009001)(7916004)(346002)(376002)(39860400002)(2980300002)(438002)(288314003)(189002)(199003)(53754006)(24454002)(13464003)(33646002)(117156002)(33716001)(5890100001)(88552002)(77096006)(106466001)(575784001)(246002)(86362001)(50466002)(2351001)(6916009)(109096001)(1730700003)(3846002)(8746002)(6116002)(3450700001)(2501003)(2950100002)(66066001)(5640700003)(6246003)(54356999)(76176999)(50986999)(8676002)(53546010)(16576012)(58126008)(786003)(316002)(83506002)(43066004)(106002)(8936002)(6306002)(9686003)(16526018)(34040400001)(189998001)(7596002)(966005)(305945005)(23676003)(6666003)(356003)(1720100001)(67846002)(47776003)(5660300001)(229853002)(2906002)(478600001)(75432002)(7736002);DIR:OUT;SFP:1102;SCL:1;SRVR:CO1PR01MB304;H:cio-socc-esr03.osuad.osu.edu;FPR:;SPF:Pass;PTR:cio-socc-esr03.osuad.osu.edu;MX:1;A:1;LANG:en; } } 18, 125 -- X-Microsoft-Exchange-Diagnostics: 1;DM3NAM05FT053;1:OZ6nQJGHB08g9VQoLWHLfuGzACtdjIwllZdysTkNjop4hM7hn6fgYrLIQ2haCikIWgQWVddN2GeA7zDbHEs1baX4ajRFuWLPpLuE0ntu88NO3epz1Stfe95TzO2WeGJI } } 18, 125 -- X-MS-PublicTrafficType: Email } } 18, 125 -- X-MS-Office365-Filtering-Correlation-Id: 6ef26a1f-c1dd-4e92-be0c-08d530e5960a } } 18, 125 -- X-Microsoft-Antispam: } } 18, 125 -- UriScan:;BCL:0;PCL:0;RULEID:(22001)(8251501002)(4534020)(4602075)(4627115)(201703031133081)(201702281549075)(2017052603258);SRVR:CO1PR01MB304; } } 18, 125 -- X-Microsoft-Exchange-Diagnostics: } } 18, 125 -- 1;CO1PR01MB304;3:qrx11n36GXcbXVKUfjNzKMQc+/4FZrkBj51uRKnsHbDAS2+/3xPX8JvJ61zB/xrN8I4L2Y89yRcuBDZMrBKDEOaUKKS4Ttl6XKLPl3CnDc3nvmtModb2OzXLE5wPHJldeYVIlfG73pSX1Nnep9VaX51y3OwR+WyOWDvCaSgcjLQsvD5DmQJ+ZV6f63R1jZMzYc9O0ay/t+avEKspzK0LI3smSiRr9qiDnygRFa7yxPiVLAWkL0kfjTXziePGvqgy+duNUYydoJZcA/ayNmm4vhGHRLpEy8gQvsewIBHvZv7CJND7oxgYKFpilcbVHOaxb4s/CrNgHsO9NJOjtlt06PtEy1g41FK1GG4RoxdKiII=;25:cQtN8tx4rGQoGi+dbFEgL699BqZblp8Glou4E6fUG92Nf4VNs6E/+uqmmcfE59EVHGIlg8uysA0T7PFSNPfCwY4MKjjsuMaoeW7oCNHVAnIJFdI4subPMZiiQ2iRDZWE3AoMRtqlrognorzKq8Rf/Rfkj4cBOgF4TO53vXE2b/bjDvQuATmowbrYIDtj6RsGaSzxj5GX2o1T0c5pZSLS5XemuIj1hNAnXw++zTB1VE3996kZu+P75TqMC+itnWDNoMcJgfySrcu12G61+lpAnOZhHUtH4zMGhS1BRVyf3qxSg7gEHwmw5o0h7WyMS3dFdZiHpEDOqcEwZP1ChyRIHQ== } } 18, 125 -- X-MS-TrafficTypeDiagnostic: CO1PR01MB304: } } 18, 125 -- X-Microsoft-Exchange-Diagnostics: } } 18, 125 -- 1;CO1PR01MB304;31:mOXzVi796wIIpc5SNHlDTokJTu4YiX4rEwPgBd6qEmUlQtsAR7TW65etFlnw2AiiY2QmhbxjzFJZryTGXsPKht2oFOIo89RV1D01QI1ntZX1jhZM1kHWDwLvsTzRrTuhuVWofR40TQ9rI1cVaG3fqTOJND+MS3ElYa+NKq8TkDXB6kfx6GLcb+qC0vxBv7p/398klHZv4lzjDDZB2PJ76NEsT2x6eyUrFIurSppDf2o=;20:z/qtoYyTUKzBIlxgBlVBcmbSDV1Z4rnjx0jP6oUIlzgDKLEt5qkaJZXoN3BpEJt/q5PdkmYc3ITLH76ly9hzl4SqmaH8mKWB1FUzoW4UwL6cDeOZ0QLqpr3B8WhzUVxK/FiowVMhjz8+IwGM+LPKy+fgqIB02uLtL78tG61givRkm3RCWlAiLyBMrn1N+zDnNl6aYF6rBi18eqE/RmOLzSYjcACCWI0HvG2nKdVviZ/MmOQpmV5nCgsAbcy/0sdnTUNhUsqwUtpuIu9xMTV47WRDO57+/LcjfjtRSgFFl2AXb4ejMtDk3YYlVfvfGJguZ5Z6g09kznpfWu9qviuebHAuKR0GPCFOoJUiiEGT3g8i1sN07kX0R66n0TlehCmSrM+vffs2D0sD9ugrx40d7t1tFxAwWETCUtrArc0yk46rr5gROpALD08Tq3Hex15ZhsazKHQUWYERSUt8j4aRe8paKc4aY/y804+6/6whoa5T4JBQTjytHiyc9G2BZ//o } } 18, 125 -- X-Microsoft-Antispam-PRVS: } } 18, 125 -- {CO1PR01MB30400734546897205B0F172DB230-at-CO1PR01MB304.prod.exchangelabs.com} } } 18, 125 -- X-Exchange-Antispam-Report-Test: } } 18, 125 -- UriScan:(181823750723407)(240347492878265)(188497347124033)(131327999870524)(211936372134217)(153496737603132)(8415204561270)(92004823454550)(17755550239193); } } 18, 125 -- X-Exchange-Antispam-Report-CFA-Test: } } 18, 125 -- BCL:0;PCL:0;RULEID:(100000700101)(100105000095)(100000701101)(100105300095)(100000702101)(100105100095)(6040450)(2401047)(8121501046)(5005006)(3231022)(920507027)(93006095)(93004095)(3002001)(100000703101)(100105400095)(10201501046)(6041248)(20161123562025)(201703131423075)(201702281529075)(201702281528075)(201703061421075)(201703061406153)(20161123564025)(20161123558100)(20161123555025)(20161123560025)(6072148)(201708071742011)(100000704101)(100105200095)(100000705101)(100105500095);SRVR:CO1PR01MB304;BCL:0;PCL:0;RULEID:(100000800101)(100110000095)(100000801101)(100110300095)(100000802101)(100110100095)(100000803101)(100110400095)(100000804101)(100110200095)(100000805101)(100110500095);SRVR:CO1PR01MB304; } } 18, 125 -- X-Microsoft-Exchange-Diagnostics: } } 18, 125 -- 1;CO1PR01MB304;4: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 } } 18, 125 -- X-Forefront-PRVS: 049897979A } } 18, 125 -- X-Microsoft-Exchange-Diagnostics: } } 18, 125 -- =?utf-8?B?MTtDTzFQUjAxTUIzMDQ7MjM6cFQvNzVNNXIzdkNRTEpkV1lTVENCaysrS0xt?= } } 18, 125 -- =?utf-8?B?TS9Kb0ZOWkVIcEJDZk1uZGZLVTVia0prVEE0dUIyL1pWbFNDUUV1NzEwUGhX?= } } 18, 125 -- =?utf-8?B?Ri9MOG5WVEVwMDVIcDMveGVCazloYnRRbTdmcjlOL2RKaG43U2w2cTFwVVlN?= } } 18, 125 -- =?utf-8?B?VVpOYVJOSXJqNWtnaUI1Tysxc0FVVUY0NEI2WUNRRTA0eDcxVndUaGE3eVBQ?= } } 18, 125 -- =?utf-8?B?enoxVVIySlpEVGRmTVVMSFIzUkhONmdnNVR0SXdlV3RySlN6L3A4c3RoQVU5?= } } 18, 125 -- =?utf-8?B?c3NGUU9CZUEvMUhLWFJ3VHRTTlVMak9Kd05VV09NTlMra01JNmszNWIxRlBk?= } } 18, 125 -- =?utf-8?B?WDdNYVF5aFpuL014K0prQlU2QllENkg3Q2RLaVVUTjEzaU1oSlVuNGFMUHJq?= } } 18, 125 -- =?utf-8?B?WU1yUUgzbk5ZU21PYXpwanI2WkNOOHd0YWpiUmZhUGkwcG9xaGhBQ1lLMXFW?= } } 18, 125 -- =?utf-8?B?SE43dEdLUUJ6S2kyMktJUEJoMFVMbmhqVEdaTGREMFA4SG1LQ25pS3lTbGRV?= } } 18, 125 -- =?utf-8?B?SkprSUhsbFB3azRJeDVQUEM1UTNlOFZlVFZkbGVkVStEbHVYOHZQZUt1Y05s?= } } 18, 125 -- =?utf-8?B?NXovdVhGWTJOSVdoLzlJRlhLQnJZdElrYWZqWU9nVGdHTER4OWJPUU9zSm43?= } } 18, 125 -- =?utf-8?B?TTR2NWwvK2pXSFg5N0dwamF3Uzc2OWRNM0NDTGJzYUQwdEROTkg1R1FablpD?= } } 18, 125 -- =?utf-8?B?b0dvODZPR2orMFY0ZUtVa2lrdXVPdlVyV05kelMrVlhWcFI4anFJLzd2OGo1?= } } 18, 125 -- =?utf-8?B?NHpJakE2TmNoUFRDNjlnUW9CZXRYVTdzWGIyWCtNaGFFUlc4MkhmTnI1OVZY?= } } 18, 125 -- =?utf-8?B?SDdjSFR6d0dJclB0Z29YUUwvY0w2S3JraXF4dHc3MThjRHdGd2NZL0ZaaVdG?= } } 18, 125 -- =?utf-8?B?WGlrZ0lvL1g4aUNycWozZVdtcUJNa2gxREFqemZFelN1dFViRzYrR0VLY1I4?= } } 18, 125 -- =?utf-8?B?QnlrQjFjTWR1MUdSTTRreitzZG5EVXRiRFZLOGlIaDdIMVo5cTVmMUJGWEZa?= } } 18, 125 -- =?utf-8?B?VFZ5TkltN01xTWtDR3pKUUhmUXdqUWFjMTMyZ0lxQWJUazM4aldEcldiZzUz?= } } 18, 125 -- =?utf-8?B?VDA3SmVmWllDRDhyS1JmTlp3SUNJUnZnM2V3L0ZhQTZSZXMvd1B3aE9qK1o3?= } } 18, 125 -- =?utf-8?B?eVhwcVlVeXNvNlNya1Fya2hTdE92Z3ZVaHR0cVRybUg2QUVhSlVTaExpdXFx?= } } 18, 125 -- =?utf-8?B?ZGo3ZzhwNmtobDc2QVkrTjd6UXNQVGp0M0ZUeTJjcnhaYWRHSHlCazFkYTZZ?= } } 18, 125 -- =?utf-8?B?cjdIVERpbnNDWlNLNy83RTJ2T2MrTXd6cko5Y1Z3UVdlK0l5WEtzWjBLazBZ?= } } 18, 125 -- =?utf-8?B?aTMrTTdFbEFBSTQ0RW0zRC9PaVpCcmsrQis1QUlodWtJQ0hwRVNvcEV6dDFG?= } } 18, 125 -- =?utf-8?B?bkRibkNKZC9BUjlMTHN4RmYvUEtKa1F6dlZ3L1lvRlZRamdLYlZvM0lnN1E1?= } } 18, 125 -- =?utf-8?B?Qi9EOWhPT0ROT0d2b01TWFVTV2FSTzJKR25vM28yYTUwWHQ4K3Nuc25wMy81?= } } 18, 125 -- =?utf-8?B?L3pVR2ZOMnVWcEM4VDlVdU5mUWpFMFdNL3FsVi9LNkpTa0lxcXpxUVZWaHJJ?= } } 18, 125 -- =?utf-8?B?Q1Y3TnJQYlZRY0M0bGczWmJLM0liQy92N1RFWXRVdkVKbUxSdWYwTk0vK0Zr?= } } 18, 125 -- =?utf-8?B?OU9OdXBLUHVIVGNWeW5WMjU4OEUxTExMbEtHRGFYS0o3SHFxNHBDTEhaZkp3?= } } 18, 125 -- =?utf-8?B?S2tRYkwvOVZ2M0RZZkJiY281NmMzOGpRMEtQdUIwRjF6aXJ5eFQraEhvSTRW?= } } 18, 125 -- =?utf-8?B?Zm4yNitlRytJUnlzdGFpK0xXZmwvRFEvNTRXOXgxNUl2TFdVbGFzcTRNNlJW?= } } 18, 125 -- =?utf-8?B?bThub1JYU2ZHbk9DRlI3Qi9SeFArSER3Y0M5c0Uvc2IyUW4wVy91OVZDNEQ5?= } } 18, 125 -- =?utf-8?B?VFpraDUwYzBpQkxMeHBlVjRxcm5mb0kybEYvZDJiNVY0QjJNTlQybzJIZVBB?= } } 18, 125 -- =?utf-8?B?RUc1SGxaWUtTSmJpNE1GSUllU2p6OFFEZXJLL2svU2RCaE90b3h0dHpyL3Jz?= } } 18, 125 -- =?utf-8?B?SzVWWFJaTXVsQWJNblRmVllVL3NMN2lTbmRseEg3M0RzS2dvYU1PVis3eWgw?= } } 18, 125 -- =?utf-8?B?Q3dWNXA5ajY5eHlFUlZKcXpHRHIwbFlDeHR5bHR5SXJHRkNGQ0tSdkxxSG9m?= } } 18, 125 -- =?utf-8?Q?3jivf2l4ImGXqBzTqBVogCDmQCzzAvyuSEXg05K3mfT?= } } 18, 125 -- X-Microsoft-Exchange-Diagnostics: } } 18, 125 -- 1;CO1PR01MB304;6:Jw6UWg+fQEFRqTByyCZfAs9fN5eK2joTEYlnhJEVnm13xi444dfVwajMYh03sb+LtWue6bKdcdn1mYKhr7mh0clUp5yOiifCL/mI1gdXl3VkDMHYotjjoo7zkBcmUwyeye5zbUWJApN8R4uvYxBaIVnK91pY0uDXwkyoJhi50E0uUoRmJaQHI1iEhnHUVXfw3yVmXHIZDwndMswTkuAsmBYZM5adl36nAV0ePUflE8iQA6pazK4Cc5+IgqHfIAq0JtvnTTVnq2vubl5aRgVZL9wSVG0Aexr26dGLVWockk09hu82WjrbmKmhqS7BCI1E32dGysgpnMDcHDyFcKv/qQBwIOli41JexjB0cOOtwHw=;5:/N/3upyWwMmgGaMws5obc5GV6fjZKBOLy9tt6Cqv7YM4cGYqqcB1KDCaLfnxFEE+t84B2XgZH0yu4hhTzegZDDM6w80oaoLx0OMx+FXLfWa2R9osO0AzgwTS6yAr87uHwgmRzyRu7Akb7RkisDZgpX1HuIeFELtQhKXNQKJsw6k=;24:109RAj66EeJEQFTr808Z5G8dIFG31jUQejKmlJeqsfjm52JFPT/gPC19yXt7tyiQk50UbG16S84zXEQbEnfiQGt9bcnJyrNL7vi4HyAngCY=;7:0js6Zo2JLBzKVbQgJ+yl+Dpzdp5+DwEwVMpcThe4vvS4fNhVC0H3lrr+TrLbvf3HDRJjoYEQPiV5uhzXu9Amb/EwFQaYf7Zcua9BILzn+mzydSTJyNfvrHmSTTymRIrikqpC0ns4I41oQ+8OdFoAUCbFOTsqHIU/ssAXXEFgHl1D/GEqCiJsJj/CthwnAx7RgLMZA0fjZhC4tBoNIQqpqhU6Js0G8tOgMdpoQCMQFy5ImWUHoq5lG4q4WMpPoGpx } } 18, 125 -- SpamDiagnosticOutput: 1:99 } } 18, 125 -- SpamDiagnosticMetadata: NSPM } } 18, 125 -- X-Microsoft-Exchange-Diagnostics: } } 18, 125 -- 1;CO1PR01MB304;20:UJDSJ79aNobTh/2+EhidyklLDe/ue+utb0idt87Zm2sG8MVMaPY2d8vO5xeb88dukg3GqV4mD7hFLpWY0ZY3dAkYnO4qJ4Klh/LlJeLHjrQKrvwuzEXeEStzNf3ynx5D0RBpdVpMPCvy6GPcn88P4IioqjywVIFCwGtLopZHxfM= } } 18, 125 -- X-OriginatorOrg: osu.edu } } 18, 125 -- X-MS-Exchange-CrossTenant-OriginalArrivalTime: 21 Nov 2017 13:41:36.4786 } } 18, 125 -- (UTC) } } 18, 125 -- X-MS-Exchange-CrossTenant-Network-Message-Id: 6ef26a1f-c1dd-4e92-be0c-08d530e5960a } } 18, 125 -- X-MS-Exchange-CrossTenant-Id: eb095636-1052-4895-952b-1ff9df1d1121 } } 18, 125 -- X-MS-Exchange-CrossTenant-OriginalAttributedTenantConnectingIp: TenantId=eb095636-1052-4895-952b-1ff9df1d1121;Ip=[128.146.138.9];Helo=[cio-socc-esr03.osuad.osu.edu] } } 18, 125 -- X-MS-Exchange-CrossTenant-FromEntityHeader: HybridOnPrem } } 18, 125 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: CO1PR01MB304 } } 18, 125 -- Content-Transfer-Encoding: 8bit } } 18, 125 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id vALDiqTJ011892 } } ==============================End of - Headers============================== } } ==============================Original Headers============================== } 34, 53 -- From microscopy.listserver-at-gmail.com Thu Nov 30 20:12:45 2017 } 34, 53 -- Received: from mail-it0-f45.google.com (mail-it0-f45.google.com [209.85.214.45]) } 34, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id vB12CjUe013727 } 34, 53 -- for {microscopy-at-microscopy.com} ; Thu, 30 Nov 2017 20:12:45 -0600 } 34, 53 -- Received: by mail-it0-f45.google.com with SMTP id r6so836560itr.3 } 34, 53 -- for {microscopy-at-microscopy.com} ; Thu, 30 Nov 2017 18:10:02 -0800 (PST) } 34, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 34, 53 -- d=gmail.com; s=20161025; } 34, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 34, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 34, 53 -- bh=WA0GrLFZfvFY6b0xpN54I0Zcz8NoECfiLdCAnLFQnEE=; } 34, 53 -- b=t9ffjTUzpAfwT8wch8XTGmtUdYffPQ8XMe4+PfRl8nNXiIm1p/6xXVqsMl2/UkIXgD } 34, 53 -- FZFoBM8EARPjPSjXhyuMHvwcagM8tkYFnPouKn/rkr8Y2GisZ4oY6aIjJzc5Ix5ZpnHE } 34, 53 -- C3MiA5TeRCwZJiWYD3FVyriePFS4kFiAhREmXpKmAXJHopJWpY7Z/CEZNE6o6HvnbntR } 34, 53 -- CMs8Pr8I/aFnm2GTo1x6lHa/hpGdmPahNfJzXaxX+n/EKlsCWzaMEWP19WvMLQfgxP8C } 34, 53 -- yrhuMXGV7VQx341ZENOg30WrUTv2q+pUcNZ9dvTiWJMA84sGD/yCBIoQ8IDW8EKhnPSy } 34, 53 -- hGQA== } 34, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 34, 53 -- d=1e100.net; s=20161025; } 34, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 34, 53 -- :user-agent:mime-version:in-reply-to:content-language } 34, 53 -- :content-transfer-encoding; } 34, 53 -- bh=WA0GrLFZfvFY6b0xpN54I0Zcz8NoECfiLdCAnLFQnEE=; } 34, 53 -- b=pZaOCughlZdenDpnMX+UfjEWNP4ZHednFTs2N3dRz9ZAdMeuvfziaVXRvF2UvYblN6 } 34, 53 -- xEFCH038XYExN15qiO77PDrGajEJuJ551HEIisCLDr8wbohjTq9TmJ/Z7b8kw579cTKg } 34, 53 -- NSkEhGWdBv90xmbH18yAuVpr8dIz1cL5SGtV9Bj1fiRuDcYhMHkDJWtibE5i2kaQAMka } 34, 53 -- AY8hhuYJnkr5J6gukD2vAUKurAI2pSNlI6F2I4ZJgZOVnKmXuWoqLH0VU58lDLNWnoPp } 34, 53 -- C0MmxSKT4NORosIQuRpOySLGVaI+xk33FtfG4Nv0o2QJ+6ceY9Ws4QilKfYNjsH0bylu } 34, 53 -- q1/A== } 34, 53 -- X-Gm-Message-State: AJaThX62poO4cRQwn00cQlOuYxfGbSOEerHZM5v///CWbrhUgNr+QWjF } 34, 53 -- ThO7Wh1j0iQQcb/lqdN/7bkvbA5u } 34, 53 -- X-Google-Smtp-Source: } AGs4zMaG9WMRsDAS9C1nUOh4Ap2J71VPcsiEX3RdjiC6Q54LChMm0xiCKz0XPKtTJCOKK/54lVmLnA== } 34, 53 -- X-Received: by 10.36.37.138 with SMTP id g132mr6512374itg.72.1512094201594; } 34, 53 -- Thu, 30 Nov 2017 18:10:01 -0800 (PST) } 34, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net } ([2603:300a:f04:7100:51c1:3512:20bc:e75f]) } 34, 53 -- by smtp.googlemail.com with ESMTPSA id k23sm3004748iti.22.2017.11.30.18.10.00 } 34, 53 -- for {microscopy-at-microscopy.com} } 34, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 34, 53 -- Thu, 30 Nov 2017 18:10:00 -0800 (PST) } 34, 53 -- Subject: Re: viaWWW:EDS and SEM projector for Outreach } 34, 53 -- References: {815C6AA8-4983-41C4-8E30-BA47AF70CDFE-at-uvic.ca} } 34, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 34, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 34, 53 -- X-Forwarded-Message-Id: {815C6AA8-4983-41C4-8E30-BA47AF70CDFE-at-uvic.ca} } 34, 53 -- Message-ID: {67ebbc40-9f7b-0546-f167-18e65e5929df-at-gmail.com} } 34, 53 -- Date: Thu, 30 Nov 2017 20:09:59 -0600 } 34, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) } 34, 53 -- Gecko/20100101 Thunderbird/52.5.0 } 34, 53 -- MIME-Version: 1.0 } 34, 53 -- In-Reply-To: {815C6AA8-4983-41C4-8E30-BA47AF70CDFE-at-uvic.ca} } 34, 53 -- Content-Type: text/plain; charset=utf-8; format=flowed } 34, 53 -- Content-Language: en-US } 34, 53 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers============================== } } ==============================Original Headers============================== } 47, 53 -- From microscopy.listserver-at-gmail.com Fri Dec 1 06:50:06 2017 } 47, 53 -- Received: from mail-io0-f176.google.com (mail-io0-f176.google.com [209.85.223.176]) } 47, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id vB1Co6xJ009130 } 47, 53 -- for {microscopy-at-microscopy.com} ; Fri, 1 Dec 2017 06:50:06 -0600 } 47, 53 -- Received: by mail-io0-f176.google.com with SMTP id s37so11061382ioe.10 } 47, 53 -- for {microscopy-at-microscopy.com} ; Fri, 01 Dec 2017 04:47:25 -0800 (PST) } 47, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 47, 53 -- d=gmail.com; s=20161025; } 47, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 47, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 47, 53 -- bh=9sBgIK0iVE/6FdyGLDvm5fkhoVyn9k//aoYLjuD9GzE=; } 47, 53 -- b=V2Cukgtq84H1b3lkm4eVnnHXKxMRXUOrPRs7OFTFUytY8z7Ug5uwWHw68dcqjRhBZP } 47, 53 -- CNnEkR8JfybSEcFVo67w7d5nyuE5Qv+s6ggBhnqVjoayNRaV8ddT/kacND51R4Sf/qKp } 47, 53 -- k150H+CDwtpTpuP1pDCCb6Cgb5QxcoPrPye2D7dnhFW8m/a7yInRP1/hZ7YfjZ+tD834 } 47, 53 -- 7B90YmcCfmuWVwUOC6Auc0Y91xfG0QrT0ZYtfHS6WmyN6x/SlsQx/JQTRvZc0+PTwNxM } 47, 53 -- E1HUU3MU0VM5jj9dTsrluMDd5qqKzoG99wI1TE5Xg9qOlm9+8AaBL984eoKZGVqufE+u } 47, 53 -- xooQ== } 47, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 47, 53 -- d=1e100.net; s=20161025; } 47, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 47, 53 -- :user-agent:mime-version:in-reply-to:content-language } 47, 53 -- :content-transfer-encoding; } 47, 53 -- bh=9sBgIK0iVE/6FdyGLDvm5fkhoVyn9k//aoYLjuD9GzE=; } 47, 53 -- b=ZxPmsAZz8GkVT2G6fR6KiUqJV8qqkMNMEX3gOvcIQdk1K3Gi47Xky3sVNErNea1QmE } 47, 53 -- pfVCX7IwRCHZ3Q1j8JkGASQkiVOTcEaDibW70RTz+RBb9tD2HAlbEQeIJyLs1vDncfNn } 47, 53 -- 4Pzvr9SYfR4HqXdbqt25MdjjgpZldNi+pfcTW4kz+HtCtG7cK62RZlLsiWyp44/VcyPV } 47, 53 -- ELDgbMpylzKyp25x0kTrhOSAzInAnnrUnYTGiDFcpsThVoqLFLu5A+6vEDodWfxJ7x+c } 47, 53 -- NlVzhp2lRpjLwahip/lXGZQ6xtaoQfWZKAimzDLP/4oIGwgpv4CbvHsCeZyO0j/Ft5vc } 47, 53 -- zbiQ== } 47, 53 -- X-Gm-Message-State: AJaThX6aNomZiAiHNt6VuHul/OqAB2xah8VQ8x+FZP6VbslXRT+NxDIm } 47, 53 -- THvY1eGrH17Ze++kaqqIivmwMdCY } 47, 53 -- X-Google-Smtp-Source: AGs4zMZs8qd/Uy8GnX7KzlgxZXkS17v5G0Ot/cyjbSSqPm+qawGBl2Q1KzlKTSG9GS9v7DX8i8D8Lg== } 47, 53 -- X-Received: by 10.107.168.216 with SMTP id e85mr12578971ioj.266.1512132443868; } 47, 53 -- Fri, 01 Dec 2017 04:47:23 -0800 (PST) } 47, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:61fd:aa9f:ced8:8289]) } 47, 53 -- by smtp.googlemail.com with ESMTPSA id 202sm3047176iou.72.2017.12.01.04.47.22 } 47, 53 -- for {microscopy-at-microscopy.com} } 47, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 47, 53 -- Fri, 01 Dec 2017 04:47:23 -0800 (PST) } 47, 53 -- Subject: Fwd: Re: [Microscopy] Re: viaWWW:EDS and SEM projector for Outreach } 47, 53 -- References: {8ds8sv60lrj33u9rv5l9f85l.1512095799901-at-email.lge.com} } 47, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 47, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 47, 53 -- X-Forwarded-Message-Id: {8ds8sv60lrj33u9rv5l9f85l.1512095799901-at-email.lge.com} } 47, 53 -- Message-ID: {c1a8686f-bdd7-9810-e43c-3061d1a81745-at-gmail.com} } 47, 53 -- Date: Fri, 1 Dec 2017 06:47:22 -0600 } 47, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) } 47, 53 -- Gecko/20100101 Thunderbird/52.5.0 } 47, 53 -- MIME-Version: 1.0 } 47, 53 -- In-Reply-To: {8ds8sv60lrj33u9rv5l9f85l.1512095799901-at-email.lge.com} } 47, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 47, 53 -- Content-Language: en-US } 47, 53 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers============================== }
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both kevin-at-semionco.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: kevin-at-semionco.com Name: Kevin Filter
Organization: Semion llc
Title-Subject: [Filtered] LEO 1450 VP SEM users
Message: Hello Listserver community. I would like to connect with other users of LEO 1450 VP SEMs and similar LEOs and Tungsten Zeiss instruments. I need some help learning the quirks of this machine and maybe getting some hints to optimize performance. If you use a LEO 1450 VP or similar instrument and are willing to take some questions please contact me. Thanks, Kevin
Login Host: 73.157.144.155 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both duleyml-at-miamioh.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: duleyml-at-miamioh.edu Name: matthew duley
Organization: Center for Advanced Microscopy & Imaging / Miami University
Title-Subject: [Filtered] coating for Carbon evaporator bell jar
Message: Afternoon all,
In a previous post I asked what everyone was doing for coating the bell jar on your carbon evaporator, now that Bell Bright wasnt going to be available. It seemed the general consensus was to use dish soap as a replacement. Well, Ive exhausted my hoard and like a dummy I didnt ask for how you did that. So the obvious questions are what are you using instead of Bell Bright and how do you apply it?
TIA
Matthew L. Duley
Microscopy Specialist Center for Advanced Microscopy & Imaging 9B Upham Hall Miami University Oxford, Ohio 45056 513.529.4164 FAX 513.529.4243 Duleyml-at-muohio.edu
Login Host: 134.53.235.185 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
From irapier044xiuea-at-gmail.com Sun Dec 3 08:35:15 2017 Return-Path: {irapier044xiuea-at-gmail.com} Received: from gmail.com (hn.kd.pix [219.155.154.242] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id vB3EZEvV014254 for {microscopylistserverarchive6-at-microscopy.com} ; Sun, 3 Dec 2017 08:35:14 -0600 Received: from rly04.hottestmile.com ([Sun, 03 Dec 2017 22:31:14 +0800]) by snmp.otwaloow.com with ESMTP; Sun, 03 Dec 2017 22:31:14 +0800 Received: from mailout.endmonthnow.com ([Sun, 03 Dec 2017 22:21:08 +0800]) by mtu67.syds.piswix.net with LOCAL; Sun, 03 Dec 2017 22:21:08 +0800 Received: from unknown (HELO snmp.otwaloow.com) (Sun, 03 Dec 2017 22:20:02 +0800) by mts.locks.grgtween.net with ESMTP; Sun, 03 Dec 2017 22:20:02 +0800 Received: from group21.345mail.com ([190.3.177.23]) by relay37.vosimerkam.net with NNFMP; Sun, 03 Dec 2017 22:05:56 +0800 Received: from mtu23.bigping.com ([Sun, 03 Dec 2017 22:01:05 +0800]) by smtp4.cyberemailings.com with ESMTP; Sun, 03 Dec 2017 22:01:05 +0800 Message-ID: {71E17AFA.61A4CB94-at-gmail.com}
X-from: John Nailon {jvnailon-at-gmail.com}
G'day Matthew,
It is very simple and inexpensive, clean your bell jar, electrodes, and insulators as best you can then wipe the inside with a piece of paper towel that has had 5-10ml of dishwashing detergent applied to it. Wipe the whole of the glass, metal and insulator surfaces so that there is a very thin smear of detergent applied. Pump down the bell jar to dry the system out. The system is ready to use! Next time you need to clean the system all you need do is wash all surfaces with warm water, dry them off with paper towel, then apply the detergent again.
Regards John V Nailon Retired Electron Microscopist
John V Nailon Mob: 0423 020 680 Email: jvnailon-at-gmail.com {mailto:jvnailon-at-gmail.com}
On Sat, Dec 2, 2017 at 12:11 PM, {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote:
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver On-Line {http://www.microscopy.com/MicroscopyListserver On-Line} Help http://www.microscopy.com/MicroscopyListserver/FAQ.html {http://www.microscopy.com/MicroscopyListserver/FAQ.html} ----------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html {http://www.microscopy.com/MLFormMail.html} --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both duleyml-at-miamioh.edu {mailto:duleyml-at-miamioh.edu} as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: duleyml-at-miamioh.edu {mailto:duleyml-at-miamioh.edu} Name: matthew duley
Organization: Center for Advanced Microscopy & Imaging / Miami University
Title-Subject: [Filtered] coating for Carbon evaporator bell jar
Message: Afternoon all,
In a previous post I asked what everyone was doing for coating the bell jar on your carbon evaporator, now that Bell Bright wasn’t going to be available. It seemed the general consensus was to use dish soap as a replacement. Well, I’ve exhausted my hoard and like a dummy I didn’t ask for how you did that. So the obvious questions are what are you using instead of Bell Bright and how do you apply it?
TIA
Matthew L. Duley
Microscopy Specialist Center for Advanced Microscopy & Imaging 9B Upham Hall Miami University Oxford, Ohio 45056 513.529.4164 {tel:513.529.4164} FAX 513.529.4243 {tel:513.529.4243} Duleyml-at-muohio.edu {mailto:Duleyml-at-muohio.edu}
Login Host: 134.53.235.185 {tel:134.53.235.185} Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
X-from: Ambrose, Wallace W {Wallace_Ambrose-at-unc.edu}
Listers, Baltimore is a great city, some faults notwithstanding. It has a wealth of old buildings and structures that have built up many layers of paint. I collected a nice thick paint sample (many layers) from a lamp post near Fell's Point to compare with some samples I collected on our campus. This was used in the SEM/EDS section of a graduate instrumentation class. I called it " The Doorway, The Downspout and the Lamp Post". It was a great introduction to examining various colors and constituents in paint layers, as well as to restoration processes. Lead paints could be dated as pre late 1950s. Wallace Ambrose Applied Physical Sciences University or North Carolina
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Friday, December 01, 2017 8:16 PM To: Ambrose, Wallace W {Wallace_Ambrose-at-unc.edu}
X-from: KEN Livi {klivi-at-jhu.edu}
Unfortunately the McCormick warehouses moved up to the north side of the city many years ago. I miss the smells that were there by the Inner Harbor. There is also a railroad theme in that the first railroad station (B&O) is situated just west of Baltimore. There could be steel, coal, and slag involved. There is the Johns Hopkins Hospital with a plethora of medical samples (blood?). The Chesapeake Bay has lots of biota. There is the infamous Chromium smelting talus that the Inner Harbor is partially built on (maybe you don’t want to have samples of Hexavalent Chromium around). Also, the Babe Ruth Museum for a baseball theme (including the Orioles). The Poe theme can have both raven’s feather and Ravens leather. To add a twist to that John Astin (Gomez Addams from the Addam Family) was born here and teaches at Johns Hopkins.
Then there is history of Frederick Douglas and the Underground Railroad.
Fort McHenry where the Star Spangled Banner was penned by Francis Scott Key and the flag sewn by Mary Pickersgill in 1813 that resides in the Smithsonian. There are cloth fibers (red and blue wool and white cotton) and gun powder themes here.
Enoch Pratt Free Library — one of the oldest libraries in the US, Peabody Library focused on music (paper, vinyl, tape).
There’s much more but I have to do some work today.
Ken
Kenneth JT Livi, PhD Director, Materials Characterization and Processing Center Materials Science and Engineering 3400 N Charles Street Johns Hopkins University Baltimore, Maryland 21218 USA
} On Dec 1, 2017, at 7:55 AM, microscopy.listserver-at-gmail.com wrote: } } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: Colijn, Hendrik {colijn.1-at-osu.edu} } } } } Edgar Allan Poe theme? } The Murders in the Rue Morgue? } Probably not "the pit and the pendulum" or "the cask of amontillado" } } Not sure what you could do with crab cakes! I believe that McCormick's has a large spice facility } near the harbor front. We could smell it the last time we were there. } } Cheers, } Henk } } Sent from my Verizon 4G LTE Smartphone } } ------ Original message------ } *From: *microscopy.listserver-at-gmail.com } *Date: *Thu, Nov 30, 2017 9:12 PM } *To: *Colijn, Hendrik; } *Cc: * } *Subject:*[Microscopy] Re: viaWWW:EDS and SEM projector for Outreach } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: Elaine Humphrey {ech-at-uvic.ca} } To: annie.muske-dukes-driggs-at-lonza.com {annie.muske-dukes-driggs-at-lonza.com} } } Hi Annie and Henk } Thank you so much for this question. I am always in need of good ideas. } } We usually have a Solve the Mystery in the Family Affair, which is changing its name this year to } Microscopic Explorations, for the delegates families and friends at the M&M conference. } } The pattern has developed whereby in a session on the Wednesday afternoon, it typically starts with } an introduction and then a lot of hands on activities using loupes and light microscopes adapted } from the GEMS Microscopical Explorations activities. At least three of the stations have a solve } the mystery. At the next meeting in Baltimore we are developing one step further and we hope to } have Fold scopes and micro meteorites. } } Then the participants go to the vendor hall where we usually have access to four sems to Solve the } Mystery. } } I have tried to correlate the mystery with the place and change it each year. For instance the CSI } Albuquerque story was: The Governor of New Mexico is coming to town. He is a baseball fan and his } favorite team is the Albuquerque Isotopes. } He was going to have his photograph taken with the cup the Isotopes just won! But disaster struck! } Someone stole the cup! It is very important we get the cup back. We have to find out who did it. } The thief left several clues at the scene of the crime: } Some pollen } Some hair } Some sand } Some fibres } } He/she wasnt a very good thief! } } } } There were the six suspects. We decided we could not use real people as suspects so we chose to go } with Harry Potter villains } Delores Umbridge } When she was interviewed Delores Umbridge was wearing a red flower. She has brown straight hair; She } had sandy shoes because she played golf and her golf ball went into a sandy bunker; She was dressed } in pink clothes. } Bellatrix Lestrange } } When she was interviewed: Bellatrix Lestrange was wearing a pink flower; she has brown straight } hair; she had sandy shoes because she was in the garden; she was wearing blue clothes. } Draco Malfoy } When he was interviewed Draco Malfoy was wearing a lily flower; he has blond straight hair; he had } sandy shoes because he took a short cut through the sand box in the garden; he was wearing grey clothes. } Voldemort } } When he was interviewed: Voldemort was wearing a red flower; no hair; he had sandy shoes because he } went hiking along a trail in California; he was wearing grey clothes. } Lucius Malfoy } } When he was interviewed: Lucius Malfoy was wearing a pink flower; he has blond straight hair; he had } sandy shoes because he went hiking along a trail in California; he was wearing black clothes } Severus Snape } } When he was interviewed: Severus Snape was wearing a lily flower; he has straight grey hair; he } had sandy shoes because he played golf and his golf ball went into a sandy bunker; he was wearing } black clothes } } } } Mostly the clues fit whatever materials we had, such as what three flowers with lots of pollen were } available to us that day. Two suspects for each clue. } } } We didnt want one clue to give the suspect away. It had to be two clues. Each group took on one } type of material. On the stub there were three possibles and the actual to compare. So we had a } flipchart at headquarters (the Outreach Booth) with the suspects down one side .and clues along the } top. As each of the four groups got their answer they put a tick in the right box. It worked just fine. } } In Portland we had access to an X-ray analysis sem. The story was: } } Someone came to the desk in the Museum and handed an envelope over to the cashier. Written on the } envelope was Put all the cash in the envelope and dont say anything. Ive got a gun! } She complied but followed him to see where he went. He took a bicycle and went through a } construction site (sand), shaped the bike along a wall (metal for X-ray analysis), over a flower bed } (pollen) which had thorny rose bushes (fibres). By that evening the police had four suspects each } with sandy shoes and bike wheels, different materials making up the bikes etc etc. } } In Nashville: Someone stole a very valuable Elvis guitar and put it up for sale clandestinely. One } of the potential buyers wanted it authenticated by identifying the strings by X-ray analysis. I went } to our local music store and asked if they had any strings from 1960s. They gave me one and I bought } a new one. The composition is very different and easy to identify by X-ray analysis. } } Now I need a story for Baltimore. Ive never been to Baltimore. Is there something special we can } use in a story? } Best wishes } } Elaine } } Dr. Elaine C. Humphrey } Advanced Microscopy Facility } Bob Wright Science Centre A015 } University of Victoria, Canada } Lab: 250-853-3968 } cell: 250-886-2068 } website: http://www.stehm.uvic.ca } } } } } } } } } } } Hi Annie, et al. } } } } I did a CSI type demo using light bulb filaments. The scenario was that } } there was a traffic accident in the dark and each driver claimed the } } others headlights were not on. The headlamps in both cars were broken. } } The 2nd driver claims that the 1st driver turned on his headlights after } } the bulbs were broken in order to cover his fault. The officer collected } } the light bulb filaments from the cars and you are comparing them in the } } SEM. (You can spin out the story as much as you want!) } } } } I took 2 small incandescent bulbs (15 watt if I remember), broke the } } glass on one and then applied power so that it burned out. I took the } } 2nd and broke the glass while it was running. I then mounted the 2 } } filaments on an SEM sticky tab and put them in the scope. } } } } Note: be careful about breaking the glass. Do it safely! I wore } } safety glasses and leather gloves and used a pair of channel pliers to } } hold the bulb inside a mostly closed cardboard box to contain the glass } } fragments. } } } } On examination, the student will find small spherical globs on one } } filament and none on the other. Doing EDX shows that the spherical } } globs are Si and O (i.e. glass that melted). The inference is that the } } filament was hot when the glass was broken, hence the light bulb was on. } } The other bulb is just oxidized and doesn't show any melted glass. } } Hence it was not running when the glass broke and was burned out } } afterwards. } } } } They seemed to enjoy the "real life" problem solving. } } } } Cheers, } } Henk } } } } } } } } } } -------------------- } } } } Hendrik O. Colijn } } Center for Electron Microscopy and AnalysiS } } The Ohio State University } } 1305 Kinnear Rd, Suite 100, Columbus, OH 43212 } } } } colijn.1-at-osu.edu 614/643-3458 } } cemas.osu.edu } } } } "Time is that quality of nature which keeps things from happening all at } } once." (Ray Cummings - 1922) } } Lately it doesn't seem to be working. } } } } ------ Original Message ------ } } X-from: microscopy.listserver-at-gmail.com } } To: colijn.1-at-osu.edu } } Sent: 11/20/2017 8:38:57 PM } } Subject: [Microscopy] viaWWW:EDS and SEM projector for Outreach } } } } } } } } } } } } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } } America } } } To Subscribe/Unsubscribe -- } } } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } } } X-from: Greg Baty {gbaty-at-pdx.edu} } } } } } } } } } Annie, } } } } } } I usually deal with kids in the 12 - 18 YO range for outreach.ย In the } } } past we offered a week long } } } half day experience in the lab.ย In my experience it is critical to } } } find samples that kids can } } } relate to. } } } } } } Insects can be a good for imaging.ย My favorites are honey bees, house } } } fly's and fruit fly's.ย Kids } } } really like the structure of the eye. } } } } } } Epsom salt has a nice structure and is useful for EDX; however, it can } } } take a long time to get } } } vacuum since it is usually hydrated.ย Best to only use a few crystals } } } or prepump. } } } } } } Geological samples.ย I prefer a thin section to reduce pump time, but } } } bulk sections work too.ย If } } } you need a thin section I suspect Spectrum Petrographics could help. I } } } have no financial connection } } } to them, but I know several of our geology Professors have Spectrum do } } } their mounts. } } } } } } I had one student bring hair and feathers from the farm she lives on.ย } } } There is a significant } } } difference between animals. } } } } } } House hold dust can be a good sample.ย Just pick a spot like a window } } } sill and don't clean it for a } } } week or two.ย Collect the sample with C tape on a stub.ย I have seen } } } pollen, insect parts and } } } silicate minerals. } } } } } } Greg } } } } } } On Fri, Nov 17, 2017 at 3:49 PM, {microscopy.listserver-at-gmail.com } } } {mailto:microscopy.listserver-at-gmail.com} } wrote: } } } } } } } } } } } } } } } } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor:ย The Microscopy Society of } } } America } } } Toย Subscribe/Unsubscribe -- } } } http://www.microscopy.com/MicroscopyListserver } } } On-Line {http://www.microscopy.com/MicroscopyListserver On-Line} } } } Help } } } http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } {http://www.microscopy.com/MicroscopyListserver/FAQ.html} } } } } } } ---------------------------------------------------------------------------- } } } } } } X-from: annie.muske-dukes-driggs-at-lonza.com } } } {mailto:annie.muske-dukes-driggs-at-lonza.com} } } } } } } This Question/Comment was submitted to the Microscopy Listserver } } } using the WWW based Form at } } } http://www.microscopy.com/MLFormMail.html } } } {http://www.microscopy.com/MLFormMail.html} } } } } } } --------------------------------------------------------------------------- } } } Remember this posting is most likely not from a Subscriber, so when } } } replying pleaseย copyย both annie.muske-dukes-driggs-at-lonza.com } } } {mailto:annie.muske-dukes-driggs-at-lonza.com} as well } } } asย ย the Microscopy Listserver } } } } } } --------------------------------------------------------------------------- } } } } } } Email: annie.muske-dukes-driggs-at-lonza.com } } } {mailto:annie.muske-dukes-driggs-at-lonza.com} Name: } } } Annie Muske-Dukes } } } } } } Organization: Bend Research } } } } } } Title-Subject: [Filtered] EDS and SEM projector for Outreach } } } } } } Message: Hi everyone, } } } } } } My company runs a community outreach program for local elementary } } } school } } } students (8-10 years old). They tour our facility and see quick } } } demonstrations/lectures on a variety of topics. } } } We have had an SEM demo for years and I would like to incorporate } } } our } } } EDS but I'm having trouble coming up with samples that kids that } } } age } } } understand, especially since I only have 15 minutes per group and } } } we try } } } to go through at least four samples. } } } } } } I have generated a map of different elements in a vitamin tablet } } } which } } } went over well and gives them a good understanding of what the } } } system is } } } doing. Any recommendations for samples that generate interesting } } } maps or } } } have unexpected elements? I'll be doing the prep/high quality maps } } } ahead } } } of time so that doesn't need to be done in the 15 minutes. } } } } } } I'd also like to get a wireless projector to use with our Hitachi } } } SU3500 } } } to project a live image of the screen onto the wall. Our service } } } rep } } } said some models can cause driver issues so if anyone here has a } } } model } } } that works please let me know. We need to stay under US$500, but } } } under } } } $300 would be ideal. } } } } } } Thanks, } } } Annie (I am a list subscriber but our required corporate } } } attachments } } } prevent me from sending the usual way) } } } } } } ย Login Host: 64.47.109.63 } } } ย Listserver Email Form V - 20120416 } } } } } } --------------------------------------------------------------------------- } } } } } } } } } } } } ==============================Original } } } Headers============================== } } } 13, 53 -- From microscopy.listserver-at-gmail.com } } } {mailto:microscopy.listserver-at-gmail.com} Fri Nov } } } 17 17:18:56 2017 } } } 13, 53 -- Received: from mail-pf0-f175.google.com } } } {http://mail-pf0-f175.google.com} } } } (mail-pf0-f175.google.com {http://mail-pf0-f175.google.com} } } } [209.85.192.175]) } } } 13, 53 --ย ย ย ย by microscopy.com {http://microscopy.com} } } } (8.12.11.20060308/8.12.8) with ESMTP } } } id vAHNItoN009038 } } } 13, 53 --ย ย ย ย for {microscopy-at-microscopy.com } } } {mailto:microscopy-at-microscopy.com} } ; Fri, 17 Nov } } } 2017 17:18:55 -0600 } } } 13, 53 -- Received: by mail-pf0-f175.google.com } } } {http://mail-pf0-f175.google.com} with SMTP id } } } x7so2994822pfa.1 } } } 13, 53 --ย ย ย ย ย for {microscopy-at-microscopy.com } } } {mailto:microscopy-at-microscopy.com} } ; Fri, 17 } } } Nov 2017 15:15:30 -0800 (PST) } } } 13, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } } } 13, 53 --ย ย ย ย ย d=gmail.com {http://gmail.com} ; s=20161025; } } } 13, 53 --ย ย ย ย ย } } } h=subject:references:to:from:message-id:date:user-agent:mime-version } } } 13, 53 --ย ย ย ย ย } } } :in-reply-to:content-language:content-transfer-encoding; } } } 13, 53 --ย ย ย ย ย } } } bh=ajHXaowS7Xr4ulq5L7g6cSgXpHzLsIrp0ixP0W269Yg=; } } } 13, 53 --ย ย ย ย ย } } } b=eAW4Xc2CZ5KfsTKhl5W7M/p2Y/WXg6iJXwJQo9hPbmsciy3Vck/Ax2o7BirKvnpdNX } } } 13, 53 --ย ย ย ย ย } } } OHTQaVaboEyxaXc7fXVUBMmkkmN+iV6G180P48hIK3TBBvUpZT2G4LwsRl/uSiTcn+nz } } } 13, 53 --ย ย ย ย ย } } } x5fEA9ygmixMEaa6gk6e/kYBbOHHJY91XyIfC5mpUS8mYQd9W03hYSd9j4792grF6hYh } } } 13, 53 --ย ย ย ย ย } } } Y6iq/B2scCSA1R0hxix7wW4L+fJUpFRTNthPfi3uuMdf9+Nmi1yAEC+3Pi9kr69xnL4U } } } 13, 53 --ย ย ย ย ย } } } 8rmapnMno5eW0z7bA3tUYDUxmCzw9TSppsLLY02yiL+Qzvt+7X6TZQGvW3MeDZynUCL4 } } } 13, 53 --ย ย ย ย ย Jd5Q== } } } 13, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; } } } c=relaxed/relaxed; } } } 13, 53 --ย ย ย ย ย d=1e100.net {http://1e100.net} ; s=20161025; } } } 13, 53 --ย ย ย ย ย } } } h=x-gm-message-state:subject:references:to:from:message-id:date } } } 13, 53 --ย ย ย ย ย } } } :user-agent:mime-version:in-reply-to:content-language } } } 13, 53 --ย ย ย ย ย :content-transfer-encoding; } } } 13, 53 --ย ย ย ย ย } } } bh=ajHXaowS7Xr4ulq5L7g6cSgXpHzLsIrp0ixP0W269Yg=; } } } 13, 53 --ย ย ย ย ย } } } b=UE5U+qloOyYI02cv3X62Aen7ygRVtpC9be1euW6ToL9RWOUNDzJuYicaiYHteGM+Qd } } } 13, 53 --ย ย ย ย ย } } } qtJ39xxJ5fCRtWOA/2craX4llZDjvyEvBu8qvgFEu0rF1s6Iq6mVP22f81Ue/sr9d/IR } } } 13, 53 --ย ย ย ย ย } } } 6fP5utPLZ77wf8zE03W8OF7aAh6jcs2CqcLdDOKut29d29W/tXo2uc/6fvFGZfrm0KC4 } } } 13, 53 --ย ย ย ย ย } } } Y8escGfPPpC2PjflO0YYjziQs6DRvEKHz2P6szOnnILH98LcBZW5PuiKEXBwpNSTgFMj } } } 13, 53 --ย ย ย ย ย } } } Fq8fQCSelKePgB2xQ4HTqJJWfkTSxsRX+YTD+e8VukuWKoI90w/IYGW2+8/ep8UuppN7 } } } 13, 53 --ย ย ย ย ย bMQA== } } } 13, 53 -- X-Gm-Message-State: } } } AJaThX6HbDIzxLHVVc7CeFhyQ7NEofsBNhRwjrnchA1onNOMwoQ/xU4k } } } 13, 53 --ย ย ย ย GlNT9VefdH3phrwGEcxoF+YUtwv8 } } } 13, 53 -- X-Google-Smtp-Source: } } } } } } AGs4zMa17Mah5ciiqmYbjFsgBJIMp4Q9FQSWDIx5LCnxz99Kp3+HdN2/YzJUDJMfrLF91MumAUhpXQ== } } } 13, 53 -- X-Received: by 10.84.193.129 with SMTP id } } } f1mr6807886pld.317.1510960529479; } } } 13, 53 --ย ย ย ย ย Fri, 17 Nov 2017 15:15:29 -0800 (PST) } } } 13, 53 -- Received: from } } } Nestors-MacBookAir-Pro-2014-ElCapitan-OSX-1163.local ([182.16.234.14]) } } } 13, 53 --ย ย ย ย ย by smtp.googlemail.com } } } {http://smtp.googlemail.com} with ESMTPSA id } } } p19sm9352707pfj.140.2017.11.17.15.15.24 } } } 13, 53 --ย ย ย ย ย for {microscopy-at-microscopy.com } } } {mailto:microscopy-at-microscopy.com} } } } } 13, 53 --ย ย ย ย ย (version=TLS1_2 } } } cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } } } 13, 53 --ย ย ย ย ย Fri, 17 Nov 2017 15:15:24 -0800 (PST) } } } 13, 53 -- Subject: viaWWW:EDS and SEM projector for Outreach } } } 13, 53 -- References: {201711162229.vAGMTWp6026431-at-microscopy.com } } } {mailto:201711162229.vAGMTWp6026431-at-microscopy.com} } } } } 13, 53 -- To: MicroscopyListServer-Forward } } } {microscopy-at-microscopy.com } } } {mailto:microscopy-at-microscopy.com} } } } } 13, 53 -- From: Microscopy Listserver } } } {microscopy.listserver-at-gmail.com } } } {mailto:microscopy.listserver-at-gmail.com} } } } } 13, 53 -- X-Forwarded-Message-Id: } } } {201711162229.vAGMTWp6026431-at-microscopy.com } } } {mailto:201711162229.vAGMTWp6026431-at-microscopy.com} } } } } 13, 53 -- Message-ID: } } } {4230175d-0ecf-bad3-c4f0-4f12f99a8f95-at-gmail.com } } } {mailto:4230175d-0ecf-bad3-c4f0-4f12f99a8f95-at-gmail.com} } } } } 13, 53 -- Date: Sat, 18 Nov 2017 07:15:22 +0800 } } } 13, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; } } } rv:52.0) } } } 13, 53 --ย Gecko/20100101 Thunderbird/52.4.0 } } } 13, 53 -- MIME-Version: 1.0 } } } 13, 53 -- In-Reply-To: {201711162229.vAGMTWp6026431-at-microscopy.com } } } {mailto:201711162229.vAGMTWp6026431-at-microscopy.com} } } } } 13, 53 -- Content-Type: text/plain; charset=windows-1252; } } } format=flowed } } } 13, 53 -- Content-Language: en-US } } } 13, 53 -- Content-Transfer-Encoding: 7bit } } } ==============================End of - } } } Headers============================== } } } } } } } } } } } } } } } -- } } } Greg Baty } } } Center for Electron Microscopy and Nanofabrication } } } Portland State University } } } 503-725-2867 } } } www.pdx.edu/cemn {http://www.pdx.edu/cemn} {http://www.pdx.edu/cemn} } } } } } } ==============================Original } } } Headers============================== } } } 33, 53 -- From microscopy.listserver-at-gmail.com Mon Nov 20 19:36:44 2017 } } } 33, 53 -- Received: from mail-it0-f44.google.com } } } (mail-it0-f44.google.com [209.85.214.44]) } } } 33, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } } } vAL1aix6015707 } } } 33, 53 -- for {microscopy-at-microscopy.com} ; Mon, 20 Nov 2017 19:36:44 } } } -0600 } } } 33, 53 -- Received: by mail-it0-f44.google.com with SMTP id } } } m191so94474itg.2 } } } 33, 53 -- for {microscopy-at-microscopy.com} ; Mon, 20 Nov 2017 } } } 17:33:29 -0800 (PST) } } } 33, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } } } 33, 53 -- d=gmail.com; s=20161025; } } } 33, 53 -- } } } h=subject:references:to:from:message-id:date:user-agent:mime-version } } } 33, 53 -- } } } :in-reply-to:content-language:content-transfer-encoding; } } } 33, 53 -- bh=/F/T7b2ateC2XsLEfi9RkPQP3HXTOGoFcz53PnVgcoI=; } } } 33, 53 -- } } } b=OUO/SyStT9xPZ4r89GrT6EjUPBuRFC9l8vd8xkOc1Wayxalbb9C+mqPP8/NWgzg4dr } } } 33, 53 -- } } } OFqE4alBS0KMSSZJWmfohnFbhtSIysENTeR0FE7ee0HlBbRx8g3OtefAKNBRtvFIuOLo } } } 33, 53 -- } } } /LEFzS+mh07DGmii2Nt4o4wD7ne032P5IgmULSN20Q3n5Lovj1MV6YxhrnND8UYy7a7W } } } 33, 53 -- } } } 7otkQyD2rFUKvJbM+25NDXNpqX5Ed3I0q/Iqe4T4WHOILvz9t+gaK8WJcEEMalcez8Uv } } } 33, 53 -- } } } 7s6JnCML7lc2Q460Kw0ERmhJxcMA2WVkOlh6oTL7c0w3uxgFYqWLiXmK0Nj2yePWqpq2 } } } 33, 53 -- 1OoQ== } } } 33, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; } } } c=relaxed/relaxed; } } } 33, 53 -- d=1e100.net; s=20161025; } } } 33, 53 -- } } } h=x-gm-message-state:subject:references:to:from:message-id:date } } } 33, 53 -- } } } :user-agent:mime-version:in-reply-to:content-language } } } 33, 53 -- :content-transfer-encoding; } } } 33, 53 -- bh=/F/T7b2ateC2XsLEfi9RkPQP3HXTOGoFcz53PnVgcoI=; } } } 33, 53 -- } } } b=OWSg9XzDy3+XzUaRg0F2VSbucdrW8e7H0aNa/LfSDSy6Lji8rYEwlhaTkyAmYxyQUv } } } 33, 53 -- } } } t9x4ytLuAQE0LM4zJ9up24/aP6n9NQXyVFuaBV1VFQsuyuNSS+rM63qSYTBUm01lbOUZ } } } 33, 53 -- } } } BfCvXhOorCKmqW88mAREkaAni+B/Bmzib/EU1iJ0p4h+byUgHZTBV2gESUKNUnJmZXEc } } } 33, 53 -- } } } DOY9s/4V8S+0KRIEhnFMvFNg1fzgs38kW+mxZPfJQuVLGCqhvm35HDopnwUYUJABHAF6 } } } 33, 53 -- } } } /8lDAfgbFMXibj91tzreRatHG4nnRL+JsC4vHNp6CLzWFixF/rcWJrKKytOpX3Q5j425 } } } 33, 53 -- ZiyA== } } } 33, 53 -- X-Gm-Message-State: } } } AJaThX7iBJBYRYoJTq5wwwtvFSYsD7iJMYdUhjmpliq/ss7rDLBowdE7 } } } 33, 53 -- 530duzpT5Kmv1fRzwmKDnbkMmt+h } } } 33, 53 -- X-Google-Smtp-Source: } } } AGs4zMb2N1wqKXFXLro1KFdZ3viGm6+WXwfo6jNXY9+C4sI9x1sJRj3kGV2cdvLHk/pj0f5pDIeFaQ== } } } 33, 53 -- X-Received: by 10.36.9.146 with SMTP id } } } 140mr21499245itm.54.1511228008678; } } } 33, 53 -- Mon, 20 Nov 2017 17:33:28 -0800 (PST) } } } 33, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net } } } ([2603:300a:f04:7100:c1ce:57a0:e8a:603e]) } } } 33, 53 -- by smtp.googlemail.com with ESMTPSA id } } } e203sm23529itb.32.2017.11.20.17.33.28 } } } 33, 53 -- for {microscopy-at-microscopy.com} } } } 33, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 } } } bits=128/128); } } } 33, 53 -- Mon, 20 Nov 2017 17:33:28 -0800 (PST) } } } 33, 53 -- Subject: viaWWW:EDS and SEM projector for Outreach } } } 33, 53 -- References: } } } {CAEw=MCG18nxj3Qta+5PRcScAHuM5OXTuwrPkMo=iRUfziAeVAA-at-mail.gmail.com} } } } 33, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } } } 33, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } } } 33, 53 -- X-Forwarded-Message-Id: } } } {CAEw=MCG18nxj3Qta+5PRcScAHuM5OXTuwrPkMo=iRUfziAeVAA-at-mail.gmail.com} } } } 33, 53 -- Message-ID: {eaf57f96-804f-e18c-b045-52f84b411735-at-gmail.com} } } } 33, 53 -- Date: Mon, 20 Nov 2017 19:33:27 -0600 } } } 33, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; } } } rv:52.0) } } } 33, 53 -- Gecko/20100101 Thunderbird/52.4.0 } } } 33, 53 -- MIME-Version: 1.0 } } } 33, 53 -- In-Reply-To: } } } {CAEw=MCG18nxj3Qta+5PRcScAHuM5OXTuwrPkMo=iRUfziAeVAA-at-mail.gmail.com} } } } 33, 53 -- Content-Type: text/plain; charset=utf-8; format=flowed } } } 33, 53 -- Content-Language: en-US } } } 33, 53 -- Content-Transfer-Encoding: 8bit } } } ==============================End of - } } } Headers============================== } } } } } } } } ==============================Original Headers============================== } } 18, 125 -- From colijn.1-at-osu.edu Tue Nov 21 07:44:53 2017 } } 18, 125 -- Received: from NAM03-BY2-obe.outbound.protection.outlook.com (mail-by2nam03on0105.outbound.protection.outlook.com [104.47.42.105]) } } 18, 125 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id vALDiqTJ011892 } } 18, 125 -- for {microscopy-at-microscopy.com} ; Tue, 21 Nov 2017 07:44:52 -0600 } } 18, 125 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=osu.edu; s=selector1; } } 18, 125 -- h=From:Date:Subject:Message-ID:Content-Type:MIME-Version; } } 18, 125 -- bh=3WsifJvqT6wI5Pu9/VzI8h1WplGD0FvIV60sJNucWtk=; } } 18, 125 -- b=KYRk1AVBhtQ17yHz9NT9edF/qobbjxnrToXpplk1Q6YROWqi9IkEVvSLqhjGJAgGgoN7ybS2yCMhB2qAPFQ7BKSV8OlyH9d4w1fyAUCAkZ1KRFeZbhTfEhb/n1622Q0YZ1Rz4QEYsSpVK6X9xegX9Esga9dBDYKwTM1n1jBSVIU= } } 18, 125 -- Received: from DM5PR0101CA0027.prod.exchangelabs.com (2603:10b6:4:28::40) by } } 18, 125 -- CO1PR01MB304.prod.exchangelabs.com (2a01:111:e400:1026::26) with Microsoft } } 18, 125 -- SMTP Server (version=TLS1_2, } } 18, 125 -- cipher=TLS_ECDHE_RSA_WITH_AES_256_CBC_SHA384_P256) id 15.20.218.12; Tue, 21 } } 18, 125 -- Nov 2017 13:41:36 +0000 } } 18, 125 -- Received: from DM3NAM05FT053.eop-nam05.prod.protection.outlook.com } } 18, 125 -- (2a01:111:f400:7e51::206) by DM5PR0101CA0027.outlook.office365.com } } 18, 125 -- (2603:10b6:4:28::40) with Microsoft SMTP Server (version=TLS1_2, } } 18, 125 -- cipher=TLS_ECDHE_RSA_WITH_AES_256_CBC_SHA384) id 15.20.239.5 via Frontend } } 18, 125 -- Transport; Tue, 21 Nov 2017 13:41:36 +0000 } } 18, 125 -- Authentication-Results: spf=pass (sender IP is 128.146.138.9) } } 18, 125 -- smtp.mailfrom=osu.edu; microscopy.com; dkim=none (message not signed) } } 18, 125 -- header.d=none;microscopy.com; dmarc=pass action=none header.from=osu.edu; } } 18, 125 -- Received-SPF: Pass (protection.outlook.com: domain of osu.edu designates } } 18, 125 -- 128.146.138.9 as permitted sender) receiver=protection.outlook.com; } } 18, 125 -- client-ip=128.146.138.9; helo=cio-socc-esr03.osuad.osu.edu; } } 18, 125 -- Received: from cio-socc-esr03.osuad.osu.edu (128.146.138.9) by } } 18, 125 -- DM3NAM05FT053.mail.protection.outlook.com (10.152.98.167) with Microsoft SMTP } } 18, 125 -- Server (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_256_CBC_SHA384_P256) id } } 18, 125 -- 15.20.239.4 via Frontend Transport; Tue, 21 Nov 2017 13:41:36 +0000 } } 18, 125 -- Received: from CIO-TNC-HT06.osuad.osu.edu (localhost [127.0.0.1]) } } 18, 125 -- (using TLSv1.2 with cipher AES256-SHA256 (256/256 bits)) } } 18, 125 -- (No client certificate requested) } } 18, 125 -- by cio-socc-esr03.osuad.osu.edu (Postfix) with ESMTPS id 93E5FA0 } } 18, 125 -- for {microscopy-at-microscopy.com} ; Tue, 21 Nov 2017 08:41:34 -0500 (EST) } } 18, 125 -- Received: from [192.168.10.60] (199.18.22.26) by smtp.service.osu.edu } } 18, 125 -- (164.107.81.254) with Microsoft SMTP Server (TLS) id 14.3.319.2; Tue, 21 Nov } } 18, 125 -- 2017 08:41:34 -0500 } } 18, 125 -- From: Henk Colijn {colijn.1-at-osu.edu} } } 18, 125 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} } } 18, 125 -- Subject: Re: [Microscopy] viaWWW:EDS and SEM projector for Outreach } } 18, 125 -- Date: Tue, 21 Nov 2017 03:04:53 +0000 } } 18, 125 -- Message-ID: {em4ab7558a-a8b1-4069-adf0-d64fad009cee-at-hoc-asus} } } 18, 125 -- In-Reply-To: {201711210138.vAL1cvN6018239-at-microscopy.com} } } 18, 125 -- References: {201711210138.vAL1cvN6018239-at-microscopy.com} } } 18, 125 -- Reply-To: Henk Colijn {colijn.1-at-osu.edu} } } 18, 125 -- User-Agent: eM_Client/7.1.30794.0 } } 18, 125 -- MIME-Version: 1.0 } } 18, 125 -- Content-Type: text/plain; format=flowed; charset="utf-8" } } 18, 125 -- X-Originating-IP: [199.18.22.26] } } 18, 125 -- x-header-sapphire: true } } 18, 125 -- X-CFilter-Loop: Reflected } } 18, 125 -- X-EOPAttributedMessage: 0 } } 18, 125 -- X-Forefront-Antispam-Report: } } 18, 125 -- CIP:128.146.138.9;IPV:NLI;CTRY:US;EFV:NLI;SFV:NSPM;SFS:(10019020)(6009001)(7916004)(346002)(376002)(39860400002)(2980300002)(438002)(288314003)(189002)(199003)(53754006)(24454002)(13464003)(33646002)(117156002)(33716001)(5890100001)(88552002)(77096006)(106466001)(575784001)(246002)(86362001)(50466002)(2351001)(6916009)(109096001)(1730700003)(3846002)(8746002)(6116002)(3450700001)(2501003)(2950100002)(66066001)(5640700003)(6246003)(54356999)(76176999)(50986999)(8676002)(53546010)(16576012)(58126008)(786003)(316002)(83506002)(43066004)(106002)(8936002)(6306002)(9686003)(16526018)(34040400001)(189998001)(7596002)(966005)(305945005)(23676003)(6666003)(356003)(1720100001)(67846002)(47776003)(5660300001)(229853002)(2906002)(478600001)(75432002)(7736002);DIR:OUT;SFP:1102;SCL:1;SRVR:CO1PR01MB304;H:cio-socc-esr03.osuad.osu.edu;FPR:;SPF:Pass;PTR:cio-socc-esr03.osuad.osu.edu;MX:1;A:1;LANG:en; } } 18, 125 -- X-Microsoft-Exchange-Diagnostics: 1;DM3NAM05FT053;1:OZ6nQJGHB08g9VQoLWHLfuGzACtdjIwllZdysTkNjop4hM7hn6fgYrLIQ2haCikIWgQWVddN2GeA7zDbHEs1baX4ajRFuWLPpLuE0ntu88NO3epz1Stfe95TzO2WeGJI } } 18, 125 -- X-MS-PublicTrafficType: Email } } 18, 125 -- X-MS-Office365-Filtering-Correlation-Id: 6ef26a1f-c1dd-4e92-be0c-08d530e5960a } } 18, 125 -- X-Microsoft-Antispam: } } 18, 125 -- UriScan:;BCL:0;PCL:0;RULEID:(22001)(8251501002)(4534020)(4602075)(4627115)(201703031133081)(201702281549075)(2017052603258);SRVR:CO1PR01MB304; } } 18, 125 -- X-Microsoft-Exchange-Diagnostics: } } 18, 125 -- 1;CO1PR01MB304;3:qrx11n36GXcbXVKUfjNzKMQc+/4FZrkBj51uRKnsHbDAS2+/3xPX8JvJ61zB/xrN8I4L2Y89yRcuBDZMrBKDEOaUKKS4Ttl6XKLPl3CnDc3nvmtModb2OzXLE5wPHJldeYVIlfG73pSX1Nnep9VaX51y3OwR+WyOWDvCaSgcjLQsvD5DmQJ+ZV6f63R1jZMzYc9O0ay/t+avEKspzK0LI3smSiRr9qiDnygRFa7yxPiVLAWkL0kfjTXziePGvqgy+duNUYydoJZcA/ayNmm4vhGHRLpEy8gQvsewIBHvZv7CJND7oxgYKFpilcbVHOaxb4s/CrNgHsO9NJOjtlt06PtEy1g41FK1GG4RoxdKiII=;25:cQtN8tx4rGQoGi+dbFEgL699BqZblp8Glou4E6fUG92Nf4VNs6E/+uqmmcfE59EVHGIlg8uysA0T7PFSNPfCwY4MKjjsuMaoeW7oCNHVAnIJFdI4subPMZiiQ2iRDZWE3AoMRtqlrognorzKq8Rf/Rfkj4cBOgF4TO53vXE2b/bjDvQuATmowbrYIDtj6RsGaSzxj5GX2o1T0c5pZSLS5XemuIj1hNAnXw++zTB1VE3996kZu+P75TqMC+itnWDNoMcJgfySrcu12G61+lpAnOZhHUtH4zMGhS1BRVyf3qxSg7gEHwmw5o0h7WyMS3dFdZiHpEDOqcEwZP1ChyRIHQ== } } 18, 125 -- X-MS-TrafficTypeDiagnostic: CO1PR01MB304: } } 18, 125 -- X-Microsoft-Exchange-Diagnostics: } } 18, 125 -- 1;CO1PR01MB304;31:mOXzVi796wIIpc5SNHlDTokJTu4YiX4rEwPgBd6qEmUlQtsAR7TW65etFlnw2AiiY2QmhbxjzFJZryTGXsPKht2oFOIo89RV1D01QI1ntZX1jhZM1kHWDwLvsTzRrTuhuVWofR40TQ9rI1cVaG3fqTOJND+MS3ElYa+NKq8TkDXB6kfx6GLcb+qC0vxBv7p/398klHZv4lzjDDZB2PJ76NEsT2x6eyUrFIurSppDf2o=;20: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 } } 18, 125 -- X-Microsoft-Antispam-PRVS: } } 18, 125 -- {CO1PR01MB30400734546897205B0F172DB230-at-CO1PR01MB304.prod.exchangelabs.com} } } 18, 125 -- X-Exchange-Antispam-Report-Test: } } 18, 125 -- UriScan:(181823750723407)(240347492878265)(188497347124033)(131327999870524)(211936372134217)(153496737603132)(8415204561270)(92004823454550)(17755550239193); } } 18, 125 -- X-Exchange-Antispam-Report-CFA-Test: } } 18, 125 -- BCL:0;PCL:0;RULEID:(100000700101)(100105000095)(100000701101)(100105300095)(100000702101)(100105100095)(6040450)(2401047)(8121501046)(5005006)(3231022)(920507027)(93006095)(93004095)(3002001)(100000703101)(100105400095)(10201501046)(6041248)(20161123562025)(201703131423075)(201702281529075)(201702281528075)(201703061421075)(201703061406153)(20161123564025)(20161123558100)(20161123555025)(20161123560025)(6072148)(201708071742011)(100000704101)(100105200095)(100000705101)(100105500095);SRVR:CO1PR01MB304;BCL:0;PCL:0;RULEID:(100000800101)(100110000095)(100000801101)(100110300095)(100000802101)(100110100095)(100000803101)(100110400095)(100000804101)(100110200095)(100000805101)(100110500095);SRVR:CO1PR01MB304; } } 18, 125 -- X-Microsoft-Exchange-Diagnostics: } } 18, 125 -- 1;CO1PR01MB304;4: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 } } 18, 125 -- X-Forefront-PRVS: 049897979A } } 18, 125 -- X-Microsoft-Exchange-Diagnostics: } } 18, 125 -- =?utf-8?B?MTtDTzFQUjAxTUIzMDQ7MjM6cFQvNzVNNXIzdkNRTEpkV1lTVENCaysrS0xt?= } } 18, 125 -- =?utf-8?B?TS9Kb0ZOWkVIcEJDZk1uZGZLVTVia0prVEE0dUIyL1pWbFNDUUV1NzEwUGhX?= } } 18, 125 -- =?utf-8?B?Ri9MOG5WVEVwMDVIcDMveGVCazloYnRRbTdmcjlOL2RKaG43U2w2cTFwVVlN?= } } 18, 125 -- =?utf-8?B?VVpOYVJOSXJqNWtnaUI1Tysxc0FVVUY0NEI2WUNRRTA0eDcxVndUaGE3eVBQ?= } } 18, 125 -- =?utf-8?B?enoxVVIySlpEVGRmTVVMSFIzUkhONmdnNVR0SXdlV3RySlN6L3A4c3RoQVU5?= } } 18, 125 -- =?utf-8?B?c3NGUU9CZUEvMUhLWFJ3VHRTTlVMak9Kd05VV09NTlMra01JNmszNWIxRlBk?= } } 18, 125 -- =?utf-8?B?WDdNYVF5aFpuL014K0prQlU2QllENkg3Q2RLaVVUTjEzaU1oSlVuNGFMUHJq?= } } 18, 125 -- =?utf-8?B?WU1yUUgzbk5ZU21PYXpwanI2WkNOOHd0YWpiUmZhUGkwcG9xaGhBQ1lLMXFW?= } } 18, 125 -- =?utf-8?B?SE43dEdLUUJ6S2kyMktJUEJoMFVMbmhqVEdaTGREMFA4SG1LQ25pS3lTbGRV?= } } 18, 125 -- =?utf-8?B?SkprSUhsbFB3azRJeDVQUEM1UTNlOFZlVFZkbGVkVStEbHVYOHZQZUt1Y05s?= } } 18, 125 -- =?utf-8?B?NXovdVhGWTJOSVdoLzlJRlhLQnJZdElrYWZqWU9nVGdHTER4OWJPUU9zSm43?= } } 18, 125 -- =?utf-8?B?TTR2NWwvK2pXSFg5N0dwamF3Uzc2OWRNM0NDTGJzYUQwdEROTkg1R1FablpD?= } } 18, 125 -- =?utf-8?B?b0dvODZPR2orMFY0ZUtVa2lrdXVPdlVyV05kelMrVlhWcFI4anFJLzd2OGo1?= } } 18, 125 -- =?utf-8?B?NHpJakE2TmNoUFRDNjlnUW9CZXRYVTdzWGIyWCtNaGFFUlc4MkhmTnI1OVZY?= } } 18, 125 -- =?utf-8?B?SDdjSFR6d0dJclB0Z29YUUwvY0w2S3JraXF4dHc3MThjRHdGd2NZL0ZaaVdG?= } } 18, 125 -- =?utf-8?B?WGlrZ0lvL1g4aUNycWozZVdtcUJNa2gxREFqemZFelN1dFViRzYrR0VLY1I4?= } } 18, 125 -- =?utf-8?B?QnlrQjFjTWR1MUdSTTRreitzZG5EVXRiRFZLOGlIaDdIMVo5cTVmMUJGWEZa?= } } 18, 125 -- =?utf-8?B?VFZ5TkltN01xTWtDR3pKUUhmUXdqUWFjMTMyZ0lxQWJUazM4aldEcldiZzUz?= } } 18, 125 -- =?utf-8?B?VDA3SmVmWllDRDhyS1JmTlp3SUNJUnZnM2V3L0ZhQTZSZXMvd1B3aE9qK1o3?= } } 18, 125 -- =?utf-8?B?eVhwcVlVeXNvNlNya1Fya2hTdE92Z3ZVaHR0cVRybUg2QUVhSlVTaExpdXFx?= } } 18, 125 -- =?utf-8?B?ZGo3ZzhwNmtobDc2QVkrTjd6UXNQVGp0M0ZUeTJjcnhaYWRHSHlCazFkYTZZ?= } } 18, 125 -- =?utf-8?B?cjdIVERpbnNDWlNLNy83RTJ2T2MrTXd6cko5Y1Z3UVdlK0l5WEtzWjBLazBZ?= } } 18, 125 -- =?utf-8?B?aTMrTTdFbEFBSTQ0RW0zRC9PaVpCcmsrQis1QUlodWtJQ0hwRVNvcEV6dDFG?= } } 18, 125 -- =?utf-8?B?bkRibkNKZC9BUjlMTHN4RmYvUEtKa1F6dlZ3L1lvRlZRamdLYlZvM0lnN1E1?= } } 18, 125 -- =?utf-8?B?Qi9EOWhPT0ROT0d2b01TWFVTV2FSTzJKR25vM28yYTUwWHQ4K3Nuc25wMy81?= } } 18, 125 -- =?utf-8?B?L3pVR2ZOMnVWcEM4VDlVdU5mUWpFMFdNL3FsVi9LNkpTa0lxcXpxUVZWaHJJ?= } } 18, 125 -- =?utf-8?B?Q1Y3TnJQYlZRY0M0bGczWmJLM0liQy92N1RFWXRVdkVKbUxSdWYwTk0vK0Zr?= } } 18, 125 -- =?utf-8?B?OU9OdXBLUHVIVGNWeW5WMjU4OEUxTExMbEtHRGFYS0o3SHFxNHBDTEhaZkp3?= } } 18, 125 -- =?utf-8?B?S2tRYkwvOVZ2M0RZZkJiY281NmMzOGpRMEtQdUIwRjF6aXJ5eFQraEhvSTRW?= } } 18, 125 -- =?utf-8?B?Zm4yNitlRytJUnlzdGFpK0xXZmwvRFEvNTRXOXgxNUl2TFdVbGFzcTRNNlJW?= } } 18, 125 -- =?utf-8?B?bThub1JYU2ZHbk9DRlI3Qi9SeFArSER3Y0M5c0Uvc2IyUW4wVy91OVZDNEQ5?= } } 18, 125 -- =?utf-8?B?VFpraDUwYzBpQkxMeHBlVjRxcm5mb0kybEYvZDJiNVY0QjJNTlQybzJIZVBB?= } } 18, 125 -- =?utf-8?B?RUc1SGxaWUtTSmJpNE1GSUllU2p6OFFEZXJLL2svU2RCaE90b3h0dHpyL3Jz?= } } 18, 125 -- =?utf-8?B?SzVWWFJaTXVsQWJNblRmVllVL3NMN2lTbmRseEg3M0RzS2dvYU1PVis3eWgw?= } } 18, 125 -- =?utf-8?B?Q3dWNXA5ajY5eHlFUlZKcXpHRHIwbFlDeHR5bHR5SXJHRkNGQ0tSdkxxSG9m?= } } 18, 125 -- =?utf-8?Q?3jivf2l4ImGXqBzTqBVogCDmQCzzAvyuSEXg05K3mfT?= } } 18, 125 -- X-Microsoft-Exchange-Diagnostics: } } 18, 125 -- 1;CO1PR01MB304;6:Jw6UWg+fQEFRqTByyCZfAs9fN5eK2joTEYlnhJEVnm13xi444dfVwajMYh03sb+LtWue6bKdcdn1mYKhr7mh0clUp5yOiifCL/mI1gdXl3VkDMHYotjjoo7zkBcmUwyeye5zbUWJApN8R4uvYxBaIVnK91pY0uDXwkyoJhi50E0uUoRmJaQHI1iEhnHUVXfw3yVmXHIZDwndMswTkuAsmBYZM5adl36nAV0ePUflE8iQA6pazK4Cc5+IgqHfIAq0JtvnTTVnq2vubl5aRgVZL9wSVG0Aexr26dGLVWockk09hu82WjrbmKmhqS7BCI1E32dGysgpnMDcHDyFcKv/qQBwIOli41JexjB0cOOtwHw=;5:/N/3upyWwMmgGaMws5obc5GV6fjZKBOLy9tt6Cqv7YM4cGYqqcB1KDCaLfnxFEE+t84B2XgZH0yu4hhTzegZDDM6w80oaoLx0OMx+FXLfWa2R9osO0AzgwTS6yAr87uHwgmRzyRu7Akb7RkisDZgpX1HuIeFELtQhKXNQKJsw6k=;24:109RAj66EeJEQFTr808Z5G8dIFG31jUQejKmlJeqsfjm52JFPT/gPC19yXt7tyiQk50UbG16S84zXEQbEnfiQGt9bcnJyrNL7vi4HyAngCY=;7:0js6Zo2JLBzKVbQgJ+yl+Dpzdp5+DwEwVMpcThe4vvS4fNhVC0H3lrr+TrLbvf3HDRJjoYEQPiV5uhzXu9Amb/EwFQaYf7Zcua9BILzn+mzydSTJyNfvrHmSTTymRIrikqpC0ns4I41oQ+8OdFoAUCbFOTsqHIU/ssAXXEFgHl1D/GEqCiJsJj/CthwnAx7RgLMZA0fjZhC4tBoNIQqpqhU6Js0G8tOgMdpoQCMQFy5ImWUHoq5lG4q4WMpPoGpx } } 18, 125 -- SpamDiagnosticOutput: 1:99 } } 18, 125 -- SpamDiagnosticMetadata: NSPM } } 18, 125 -- X-Microsoft-Exchange-Diagnostics: } } 18, 125 -- 1;CO1PR01MB304;20:UJDSJ79aNobTh/2+EhidyklLDe/ue+utb0idt87Zm2sG8MVMaPY2d8vO5xeb88dukg3GqV4mD7hFLpWY0ZY3dAkYnO4qJ4Klh/LlJeLHjrQKrvwuzEXeEStzNf3ynx5D0RBpdVpMPCvy6GPcn88P4IioqjywVIFCwGtLopZHxfM= } } 18, 125 -- X-OriginatorOrg: osu.edu } } 18, 125 -- X-MS-Exchange-CrossTenant-OriginalArrivalTime: 21 Nov 2017 13:41:36.4786 } } 18, 125 -- (UTC) } } 18, 125 -- X-MS-Exchange-CrossTenant-Network-Message-Id: 6ef26a1f-c1dd-4e92-be0c-08d530e5960a } } 18, 125 -- X-MS-Exchange-CrossTenant-Id: eb095636-1052-4895-952b-1ff9df1d1121 } } 18, 125 -- X-MS-Exchange-CrossTenant-OriginalAttributedTenantConnectingIp: TenantId=eb095636-1052-4895-952b-1ff9df1d1121;Ip=[128.146.138.9];Helo=[cio-socc-esr03.osuad.osu.edu] } } 18, 125 -- X-MS-Exchange-CrossTenant-FromEntityHeader: HybridOnPrem } } 18, 125 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: CO1PR01MB304 } } 18, 125 -- Content-Transfer-Encoding: 8bit } } 18, 125 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id vALDiqTJ011892 } } ==============================End of - Headers============================== } } ==============================Original Headers============================== } 34, 53 -- From microscopy.listserver-at-gmail.com Thu Nov 30 20:12:45 2017 } 34, 53 -- Received: from mail-it0-f45.google.com (mail-it0-f45.google.com [209.85.214.45]) } 34, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id vB12CjUe013727 } 34, 53 -- for {microscopy-at-microscopy.com} ; Thu, 30 Nov 2017 20:12:45 -0600 } 34, 53 -- Received: by mail-it0-f45.google.com with SMTP id r6so836560itr.3 } 34, 53 -- for {microscopy-at-microscopy.com} ; Thu, 30 Nov 2017 18:10:02 -0800 (PST) } 34, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 34, 53 -- d=gmail.com; s=20161025; } 34, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 34, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 34, 53 -- bh=WA0GrLFZfvFY6b0xpN54I0Zcz8NoECfiLdCAnLFQnEE=; } 34, 53 -- b=t9ffjTUzpAfwT8wch8XTGmtUdYffPQ8XMe4+PfRl8nNXiIm1p/6xXVqsMl2/UkIXgD } 34, 53 -- FZFoBM8EARPjPSjXhyuMHvwcagM8tkYFnPouKn/rkr8Y2GisZ4oY6aIjJzc5Ix5ZpnHE } 34, 53 -- C3MiA5TeRCwZJiWYD3FVyriePFS4kFiAhREmXpKmAXJHopJWpY7Z/CEZNE6o6HvnbntR } 34, 53 -- CMs8Pr8I/aFnm2GTo1x6lHa/hpGdmPahNfJzXaxX+n/EKlsCWzaMEWP19WvMLQfgxP8C } 34, 53 -- yrhuMXGV7VQx341ZENOg30WrUTv2q+pUcNZ9dvTiWJMA84sGD/yCBIoQ8IDW8EKhnPSy } 34, 53 -- hGQA== } 34, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 34, 53 -- d=1e100.net; s=20161025; } 34, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 34, 53 -- :user-agent:mime-version:in-reply-to:content-language } 34, 53 -- :content-transfer-encoding; } 34, 53 -- bh=WA0GrLFZfvFY6b0xpN54I0Zcz8NoECfiLdCAnLFQnEE=; } 34, 53 -- b=pZaOCughlZdenDpnMX+UfjEWNP4ZHednFTs2N3dRz9ZAdMeuvfziaVXRvF2UvYblN6 } 34, 53 -- xEFCH038XYExN15qiO77PDrGajEJuJ551HEIisCLDr8wbohjTq9TmJ/Z7b8kw579cTKg } 34, 53 -- NSkEhGWdBv90xmbH18yAuVpr8dIz1cL5SGtV9Bj1fiRuDcYhMHkDJWtibE5i2kaQAMka } 34, 53 -- AY8hhuYJnkr5J6gukD2vAUKurAI2pSNlI6F2I4ZJgZOVnKmXuWoqLH0VU58lDLNWnoPp } 34, 53 -- C0MmxSKT4NORosIQuRpOySLGVaI+xk33FtfG4Nv0o2QJ+6ceY9Ws4QilKfYNjsH0bylu } 34, 53 -- q1/A== } 34, 53 -- X-Gm-Message-State: AJaThX62poO4cRQwn00cQlOuYxfGbSOEerHZM5v///CWbrhUgNr+QWjF } 34, 53 -- ThO7Wh1j0iQQcb/lqdN/7bkvbA5u } 34, 53 -- X-Google-Smtp-Source: } AGs4zMaG9WMRsDAS9C1nUOh4Ap2J71VPcsiEX3RdjiC6Q54LChMm0xiCKz0XPKtTJCOKK/54lVmLnA== } 34, 53 -- X-Received: by 10.36.37.138 with SMTP id g132mr6512374itg.72.1512094201594; } 34, 53 -- Thu, 30 Nov 2017 18:10:01 -0800 (PST) } 34, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net } ([2603:300a:f04:7100:51c1:3512:20bc:e75f]) } 34, 53 -- by smtp.googlemail.com with ESMTPSA id k23sm3004748iti.22.2017.11.30.18.10.00 } 34, 53 -- for {microscopy-at-microscopy.com} } 34, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 34, 53 -- Thu, 30 Nov 2017 18:10:00 -0800 (PST) } 34, 53 -- Subject: Re: viaWWW:EDS and SEM projector for Outreach } 34, 53 -- References: {815C6AA8-4983-41C4-8E30-BA47AF70CDFE-at-uvic.ca} } 34, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 34, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 34, 53 -- X-Forwarded-Message-Id: {815C6AA8-4983-41C4-8E30-BA47AF70CDFE-at-uvic.ca} } 34, 53 -- Message-ID: {67ebbc40-9f7b-0546-f167-18e65e5929df-at-gmail.com} } 34, 53 -- Date: Thu, 30 Nov 2017 20:09:59 -0600 } 34, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) } 34, 53 -- Gecko/20100101 Thunderbird/52.5.0 } 34, 53 -- MIME-Version: 1.0 } 34, 53 -- In-Reply-To: {815C6AA8-4983-41C4-8E30-BA47AF70CDFE-at-uvic.ca} } 34, 53 -- Content-Type: text/plain; charset=utf-8; format=flowed } 34, 53 -- Content-Language: en-US } 34, 53 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers============================== } } ==============================Original Headers============================== } 47, 53 -- From microscopy.listserver-at-gmail.com Fri Dec 1 06:50:06 2017 } 47, 53 -- Received: from mail-io0-f176.google.com (mail-io0-f176.google.com [209.85.223.176]) } 47, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id vB1Co6xJ009130 } 47, 53 -- for {microscopy-at-microscopy.com} ; Fri, 1 Dec 2017 06:50:06 -0600 } 47, 53 -- Received: by mail-io0-f176.google.com with SMTP id s37so11061382ioe.10 } 47, 53 -- for {microscopy-at-microscopy.com} ; Fri, 01 Dec 2017 04:47:25 -0800 (PST) } 47, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 47, 53 -- d=gmail.com; s=20161025; } 47, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 47, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 47, 53 -- bh=9sBgIK0iVE/6FdyGLDvm5fkhoVyn9k//aoYLjuD9GzE=; } 47, 53 -- b=V2Cukgtq84H1b3lkm4eVnnHXKxMRXUOrPRs7OFTFUytY8z7Ug5uwWHw68dcqjRhBZP } 47, 53 -- CNnEkR8JfybSEcFVo67w7d5nyuE5Qv+s6ggBhnqVjoayNRaV8ddT/kacND51R4Sf/qKp } 47, 53 -- k150H+CDwtpTpuP1pDCCb6Cgb5QxcoPrPye2D7dnhFW8m/a7yInRP1/hZ7YfjZ+tD834 } 47, 53 -- 7B90YmcCfmuWVwUOC6Auc0Y91xfG0QrT0ZYtfHS6WmyN6x/SlsQx/JQTRvZc0+PTwNxM } 47, 53 -- E1HUU3MU0VM5jj9dTsrluMDd5qqKzoG99wI1TE5Xg9qOlm9+8AaBL984eoKZGVqufE+u } 47, 53 -- xooQ== } 47, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 47, 53 -- d=1e100.net; s=20161025; } 47, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 47, 53 -- :user-agent:mime-version:in-reply-to:content-language } 47, 53 -- :content-transfer-encoding; } 47, 53 -- bh=9sBgIK0iVE/6FdyGLDvm5fkhoVyn9k//aoYLjuD9GzE=; } 47, 53 -- b=ZxPmsAZz8GkVT2G6fR6KiUqJV8qqkMNMEX3gOvcIQdk1K3Gi47Xky3sVNErNea1QmE } 47, 53 -- pfVCX7IwRCHZ3Q1j8JkGASQkiVOTcEaDibW70RTz+RBb9tD2HAlbEQeIJyLs1vDncfNn } 47, 53 -- 4Pzvr9SYfR4HqXdbqt25MdjjgpZldNi+pfcTW4kz+HtCtG7cK62RZlLsiWyp44/VcyPV } 47, 53 -- ELDgbMpylzKyp25x0kTrhOSAzInAnnrUnYTGiDFcpsThVoqLFLu5A+6vEDodWfxJ7x+c } 47, 53 -- NlVzhp2lRpjLwahip/lXGZQ6xtaoQfWZKAimzDLP/4oIGwgpv4CbvHsCeZyO0j/Ft5vc } 47, 53 -- zbiQ== } 47, 53 -- X-Gm-Message-State: AJaThX6aNomZiAiHNt6VuHul/OqAB2xah8VQ8x+FZP6VbslXRT+NxDIm } 47, 53 -- THvY1eGrH17Ze++kaqqIivmwMdCY } 47, 53 -- X-Google-Smtp-Source: AGs4zMZs8qd/Uy8GnX7KzlgxZXkS17v5G0Ot/cyjbSSqPm+qawGBl2Q1KzlKTSG9GS9v7DX8i8D8Lg== } 47, 53 -- X-Received: by 10.107.168.216 with SMTP id e85mr12578971ioj.266.1512132443868; } 47, 53 -- Fri, 01 Dec 2017 04:47:23 -0800 (PST) } 47, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:61fd:aa9f:ced8:8289]) } 47, 53 -- by smtp.googlemail.com with ESMTPSA id 202sm3047176iou.72.2017.12.01.04.47.22 } 47, 53 -- for {microscopy-at-microscopy.com} } 47, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 47, 53 -- Fri, 01 Dec 2017 04:47:23 -0800 (PST) } 47, 53 -- Subject: Fwd: Re: [Microscopy] Re: viaWWW:EDS and SEM projector for Outreach } 47, 53 -- References: {8ds8sv60lrj33u9rv5l9f85l.1512095799901-at-email.lge.com} } 47, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 47, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 47, 53 -- X-Forwarded-Message-Id: {8ds8sv60lrj33u9rv5l9f85l.1512095799901-at-email.lge.com} } 47, 53 -- Message-ID: {c1a8686f-bdd7-9810-e43c-3061d1a81745-at-gmail.com} } 47, 53 -- Date: Fri, 1 Dec 2017 06:47:22 -0600 } 47, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) } 47, 53 -- Gecko/20100101 Thunderbird/52.5.0 } 47, 53 -- MIME-Version: 1.0 } 47, 53 -- In-Reply-To: {8ds8sv60lrj33u9rv5l9f85l.1512095799901-at-email.lge.com} } 47, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 47, 53 -- Content-Language: en-US } 47, 53 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers============================== }
that should work for you. Call and ask for Mike Bouchard if you want to discuss it.
Thanks,
JD Arnott
President
Disclaimer: Ladd research sells this product and other products for use in Microscopy Labs.
Join us on Facebook at https://www.facebook.com/pages/Ladd-Research
And Twitter at https://twitter.com/laddresearch
Ladd Research 83 Holly Court Williston, VT 05495
T- 802-658-4961 F- 802-660-8859
www.laddresearch.com
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Friday, December 01, 2017 7:58 PM To: jd-at-laddresearch.com
X-from: duleyml-at-miamioh.edu
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both duleyml-at-miamioh.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: duleyml-at-miamioh.edu Name: matthew duley
Organization: Center for Advanced Microscopy & Imaging / Miami University
Title-Subject: [Filtered] coating for Carbon evaporator bell jar
Message: Afternoon all,
In a previous post I asked what everyone was doing for coating the bell jar on your carbon evaporator, now that Bell Bright wasnÂt going to be available. It seemed the general consensus was to use dish soap as a replacement. Well, IÂve exhausted my hoard and like a dummy I didnÂt ask for how you did that. So the obvious questions are what are you using instead of Bell Bright and how do you apply it?
TIA
Matthew L. Duley
Microscopy Specialist Center for Advanced Microscopy & Imaging 9B Upham Hall Miami University Oxford, Ohio 45056 513.529.4164 FAX 513.529.4243 Duleyml-at-muohio.edu
Login Host: 134.53.235.185 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both kuryu-at-rockefeller.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Electron Microscopist Position Available at The Rockefeller University
Message: Dear list,
The Rockefeller University, a premier biomedical research institution, seeks a Research Support-at-Associate or Specialist to join our Electron Microscopy Resource Center (EMRC).
The EMRC provides state-of-the-art electron microscopy support for analysis of a wide variety of biological samples, including viruses, bacteria, insects, animal tissue as well as cultured cells and isolated cellular components for structural analyses or immuno-electron microscopy. The EMRC is equipped with three transmission electron microscopes, a conventional and a serial block-face imaging scanning electron microscope, and a high-pressure freezing and a freeze-substitution unit. (http://www.rockefeller.edu/emrc/) The Research Support Associate/Specialist will participate in all of the EMRCfs daily operations, including maintenance, upkeep and use of the electron microscopes and associated equipment, ordering supplies, interacting with vendors, and administrative support for office duties, including center billing. The position also entails specimen preparation, including negative staining, ultrathin sectioning, and immunolabeling, operation of the microscopes and associated equipment, training users, as well as consulting scientists on the design of experiments, data processing/analysis, interpretation of results, and informing users on the latest methodology through familiarity with relevant literature.
The successful candidate will have an M.S./Ph.D. degree or equivalent background in biology, bioengineering or a related field and must have a minimum of 5 years of hands-on experience in electron microscopy. A strong background in computation would be a plus. Must have strong communication skills, and the ability to work collaboratively in a team as well as independently on a wide variety of research projects. Must be detail-oriented, focused, and highly motivated.
We offer a competitive salary, comprehensive benefits, and a collegial work environment. To apply to this job, click the following URL, click on 'staff opportunitiesf and enter keyword eIRC20449f or eElectron Microscopistf: http://www.rockefeller.edu/hr/career.php
The Rockefeller University is an Affirmative Action/Equal Employment Opportunity/VEVRAA employer.
Regards,
Hiro ------
Kunihiro Uryu, Ph.D., Research Assistant Professor Director of Electron Microscopy Resource Center (EMRC) RRB Rm120 The Rockefeller University 1230 York Ave., Box 230 New York, NY 10065
Login Host: 129.85.110.225 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
==============================Original Headers============================== 18, 54 -- From microscopy.listserver-at-gmail.com Mon Dec 4 10:05:19 2017 18, 54 -- Received: from mail-it0-f45.google.com (mail-it0-f45.google.com [209.85.214.45]) 18, 54 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id vB4G5Juv015067 18, 54 -- for {microscopy-at-microscopy.com} ; Mon, 4 Dec 2017 10:05:19 -0600 18, 54 -- Received: by mail-it0-f45.google.com with SMTP id p139so13907748itb.1 18, 54 -- for {microscopy-at-microscopy.com} ; Mon, 04 Dec 2017 08:02:48 -0800 (PST) 18, 54 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 18, 54 -- d=gmail.com; s=20161025; 18, 54 -- h=subject:references:to:from:message-id:date:user-agent:mime-version 18, 54 -- :in-reply-to:content-language:content-transfer-encoding; 18, 54 -- bh=ZbgRyIctCM7EugpImgna6CvrHnwOzb3f6Zn4kTWCDTc=; 18, 54 -- b=ULpCxS6MFlJ2um17yhp84x9fH5L9mjAe8ZktUC1IfzY4AuRV1LZm1OWw5qhGKWAr4H 18, 54 -- 8SrFM5D+nLGjDwYjCcFK7ZYGtvqOKxxqMgZehKaARhMiV2BlMwED7ryT6Aokwp+IZebL 18, 54 -- WZyaYLNq2pA+2YKvtMWGtphU9Hoba7p9VZsEwJppKHw6WDZJfE6AtPpwiVvI6W1mkmLR 18, 54 -- CCyMF8ia/onJPpJgk9AL5RmBygzFpyTnt83fM9OZs3EObcJ/U1WiCdIlib/BWNg0849z 18, 54 -- PMqjy09QJyMvsRklivJxwBNa+4ojX2/V0AUEUjbwRwJl8jOUUnDMgREGXJnbjlUPFVgq 18, 54 -- 14EA== 18, 54 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 18, 54 -- d=1e100.net; s=20161025; 18, 54 -- h=x-gm-message-state:subject:references:to:from:message-id:date 18, 54 -- :user-agent:mime-version:in-reply-to:content-language 18, 54 -- :content-transfer-encoding; 18, 54 -- bh=ZbgRyIctCM7EugpImgna6CvrHnwOzb3f6Zn4kTWCDTc=; 18, 54 -- b=biEcTk0pILNIxEyxfJ7mP5sJjuto8fKJS9y2/JNd7Orfp4XR87+wIzzCwXihxI4jAH 18, 54 -- QJ9fottsWshAABdmyE7Vgg4IihhcUIknXKrR2dcY5L6yv8zulD8LIbiM1rXKxauqWpwd 18, 54 -- AIwbA+6Y7Bt/ZOkf6S/tXQRRTJL762ZMzcA0dX8Pp36oFCJbXe3m+xOYxwm1j3oT+nOt 18, 54 -- L8r1vX1kEQbJKRfXKfO/rdqFE+sq4OmWFjvdf75RACrG+5gdeI+HA41tdM1HNrx/FQAf 18, 54 -- 1eacYU2zDYkBV0Zd2cZPIpTDQ8X3X7B4NN14Vnzd8SiZKbWldpNpthoMLEwLWEGcMsNL 18, 54 -- 87Qg== 18, 54 -- X-Gm-Message-State: AJaThX4Q78GkmLCCN+hsVhL8Dysgf1pV6mlhbhIRnzcRiSjtRg8qEFGm 18, 54 -- ecpk63Zvwm2UQDG4eYzrXwhpDWiK 18, 54 -- X-Google-Smtp-Source: AGs4zMb85y5pyd5C3XQ5iFuWOqKZ6Vh2v92Y8Zho50NUZbtsB5GV4UrP1gnPLTuyxY7TUSTmBmlTIw== 18, 54 -- X-Received: by 10.107.104.18 with SMTP id d18mr22594889ioc.136.1512403367806; 18, 54 -- Mon, 04 Dec 2017 08:02:47 -0800 (PST) 18, 54 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:ed3f:36b:35d9:bd27]) 18, 54 -- by smtp.googlemail.com with ESMTPSA id f124sm1031798ioe.20.2017.12.04.08.02.46 18, 54 -- for {microscopy-at-microscopy.com} 18, 54 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 18, 54 -- Mon, 04 Dec 2017 08:02:47 -0800 (PST) 18, 54 -- Subject: viaWWW: Electron Microscopist Position Available at The Rockefeller 18, 54 -- University 18, 54 -- References: {201712041417.vB4EHcCe027594-at-microscopy.com} 18, 54 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 18, 54 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} 18, 54 -- X-Forwarded-Message-Id: {201712041417.vB4EHcCe027594-at-microscopy.com} 18, 54 -- Message-ID: {0a83416f-0d1d-1c33-89b4-242bc38d7e2c-at-gmail.com} 18, 54 -- Date: Mon, 4 Dec 2017 10:02:46 -0600 18, 54 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) 18, 54 -- Gecko/20100101 Thunderbird/52.5.0 18, 54 -- MIME-Version: 1.0 18, 54 -- In-Reply-To: {201712041417.vB4EHcCe027594-at-microscopy.com} 18, 54 -- Content-Type: text/plain; charset=windows-1252; format=flowed 18, 54 -- Content-Language: en-US 18, 54 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
I have the denton floor model carbon evaporator and I use a very small dab of metal polish on a paper towel, within a small area of the jar. I immediately chase with 100% ethanolthen move to another small region o f the jar. I have never had vac issues cleaning the bell jar like this.
Good luck, Michael Delannoy
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Friday, December 01, 2017 7:58 PM To: delannoy-at-jhmi.edu
X-from: duleyml-at-miamioh.edu
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both duleyml-at-miamioh.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: duleyml-at-miamioh.edu Name: matthew duley
Organization: Center for Advanced Microscopy & Imaging / Miami University
Title-Subject: [Filtered] coating for Carbon evaporator bell jar
Message: Afternoon all,
In a previous post I asked what everyone was doing for coating the bell jar on your carbon evaporator, now that Bell Bright wasnBt going to be available. It seemed the general consensus was to use dish soap as a replacement. Well, IBve exhausted my hoard and like a dummy I didnBt ask for how you did that. So the obvious questions are what are you using instead of Bell Bright and how do you apply it?
TIA
Matthew L. Duley
Microscopy Specialist Center for Advanced Microscopy & Imaging 9B Upham Hall Miami University Oxford, Ohio 45056 513.529.4164 FAX 513.529.4243 Duleyml-at-muohio.edu
Login Host: 134.53.235.185 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
From samjax3132bik-at-gmail.com Mon Dec 4 14:16:11 2017 Return-Path: {samjax3132bik-at-gmail.com} Received: from gmail.com ([60.172.229.94]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id vB4KG8Zm016230 for {microscopylistserverarchive6-at-microscopy.com} ; Mon, 4 Dec 2017 14:16:10 -0600 Received: from unknown (166.224.36.128) by mts.locks.grgtween.net with ESMTP; Tue, 05 Dec 2017 05:11:58 +0900 Received: from unknown (HELO mtu23.bigping.com) (Tue, 05 Dec 2017 04:55:11 +0900) by mxs.perenter.com with ESMTP; Tue, 05 Dec 2017 04:55:11 +0900 Message-ID: {D3AEDC2E.BE4E5972-at-gmail.com}
X-from: pveril-at-uth.gr
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both pveril-at-uth.gr as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: pveril-at-uth.gr Name: Panagiotis Berillis
Organization: University of Thessaly
Title-Subject: [Filtered] CM10 ODP water message
Message: Hi all Today I tried to switch on our Philips CM10 TEM. After an hour the message "ODP water" appeared on the monitor. Reading the manual I figured out that the message was about high water temperature (} 60 oC)on the ODP cooling system. There is the S30 switch that checks that temperature. Our water cooling system seems to work fine (temperature about 15oC). Any ideas how to fix the problem?
Panagiotis
Login Host: 109.242.105.72 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both driscollg2-at-winthrop.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Embedding Chick Brain (E7-E10) for Vibratome Sectioning
Message: I am doing brain electroporations with an RCASB plasmid and I wanted to visualize how efficient my electroporations were. Currently, I am embedding my heads in 3% agarose at 37 degrees between 15-20 minutes, cutting the block fairly close to the brain, and a final embedding in 6% agarose at 4 degrees for at least 20 minutes. The smallest sections I have been able to slice is 300 microns; however, I would like to get down to 200 microns. When I have attempted to go below 300 microns, the tissue gets destroyed, which I can clearly see that it's lacking structural support from how I am embedding. My question: What would be a more efficient way to embed my samples so I can section between 100-200 microns?
Login Host: 206.74.212.199 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
Check the thermal sensor on the ODP, it may need replacing. This happened on my CM200.
Nestor
} On Dec 5, 2017, at 12:13 PM CST, {microscopy.listserver-at-gmail.com} {microscopy.listserver-at-gmail.com} wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: pveril-at-uth.gr } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } pveril-at-uth.gr as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: pveril-at-uth.gr Name: Panagiotis Berillis } } Organization: University of Thessaly } } Title-Subject: [Filtered] CM10 ODP water message } } Message: Hi all } Today I tried to switch on our Philips CM10 TEM. After an hour the message "ODP water" appeared on } the monitor. Reading the manual I figured out that the message was about high water temperature (} 60 } oC)on the ODP cooling system. There is the S30 switch that checks that temperature. Our water } cooling system seems to work fine (temperature about 15oC). Any ideas how to fix the problem? } } Panagiotis } } Login Host: 109.242.105.72 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 10, 53 -- From microscopy.listserver-at-gmail.com Tue Dec 5 12:13:21 2017 } 10, 53 -- Received: from mail-it0-f47.google.com (mail-it0-f47.google.com [209.85.214.47]) } 10, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id vB5IDLsK014458 } 10, 53 -- for {microscopy-at-microscopy.com} ; Tue, 5 Dec 2017 12:13:21 -0600 } 10, 53 -- Received: by mail-it0-f47.google.com with SMTP id d16so3686235itj.1 } 10, 53 -- for {microscopy-at-microscopy.com} ; Tue, 05 Dec 2017 10:10:53 -0800 (PST) } 10, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 10, 53 -- d=gmail.com; s=20161025; } 10, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 10, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 10, 53 -- bh=Xf/zqQLl/B0GZwwQ9wGXkM8eAyBKnzS/K46zly8l7e8=; } 10, 53 -- b=LLl/YJUP8iVhs5gJ/xZvRQItsEC51phllOSsl9AS/dVJQm1pBU5WCEfRgukGHy3z6N } 10, 53 -- Xa4jvszz1jmFPCc5JVgLvzqkouYgIzPoHIGK7bnBi0CdPjapEfOur+jcDLHGzEBQR8Ud } 10, 53 -- 4mMbaQc/kCN5oWhkBSxJMMVWpimqtW6GpJ6Kat262P8Synp/bhcFMR9UmhIK/Rnr5pKa } 10, 53 -- 4vYGGZeLfZRmKQuwMZMIO63Z7vxxUAQbvsAklKWBeBKqluDggXDTtUq28S2X1j0AzEUq } 10, 53 -- WSyu3myVgR7Xpfk0l1cvvgXzgSgA62ETHFpHlZcaofA6N4CGnaSa/Pv3JYqTTmOxYNV8 } 10, 53 -- Dmkg== } 10, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 10, 53 -- d=1e100.net; s=20161025; } 10, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 10, 53 -- :user-agent:mime-version:in-reply-to:content-language } 10, 53 -- :content-transfer-encoding; } 10, 53 -- bh=Xf/zqQLl/B0GZwwQ9wGXkM8eAyBKnzS/K46zly8l7e8=; } 10, 53 -- b=p8Uw2vTQWI70cAAjQIveNpWb5djgwzAvHWPYKnXD2XZKADWh92smrF4nKpL8rZxrX1 } 10, 53 -- fG8CZusDUAt6ywpvIZn98hnurcN8jK46Vag7nj5uEtF5FhS5NtSKcJ2KppBhwxXyx3NP } 10, 53 -- D4BeW/GQyNqSavNusSZABNBeWUlVFek6vTFP9ncfDuwZAZS8EbIDtoTthd/qsuxxdqFC } 10, 53 -- Y3MEjLWgWbOIBTU45WNs+N/sTUhA7KnyXZI/Zbqnx1+LPgvV7XjaDx/cPPpYGIM6y2f9 } 10, 53 -- pLRcoPIdhWoRyuLkOnHKMaDE956ST0SpfeVs4Zt0UK+txtiioRxwXAf4zsNl0GERicq/ } 10, 53 -- yaBQ== } 10, 53 -- X-Gm-Message-State: AKGB3mL7E7gQqMVDPLP5wJhxtgpR7H5lU6FpVPaIsQmW/ybfAAUxbPbQ } 10, 53 -- 1HepvcDqOgkprNX0qEJA/Le0gEtE } 10, 53 -- X-Google-Smtp-Source: AGs4zMZ96phVLhjOHO7o23rm7Ab0u2gxy+vUNG5aE9coCZEmA3X5vCMDjXBAomJ+Me39NBFsgDIrmA== } 10, 53 -- X-Received: by 10.107.14.213 with SMTP id 204mr3688967ioo.127.1512497452509; } 10, 53 -- Tue, 05 Dec 2017 10:10:52 -0800 (PST) } 10, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:9cd6:c96d:d37c:9538]) } 10, 53 -- by smtp.googlemail.com with ESMTPSA id r17sm268773ioe.88.2017.12.05.10.10.49 } 10, 53 -- for {microscopy-at-microscopy.com} } 10, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 10, 53 -- Tue, 05 Dec 2017 10:10:50 -0800 (PST) } 10, 53 -- Subject: viaWWW: } 10, 53 -- References: {201712051615.vB5GFOLG008536-at-microscopy.com} } 10, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 10, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 10, 53 -- X-Forwarded-Message-Id: {201712051615.vB5GFOLG008536-at-microscopy.com} } 10, 53 -- Message-ID: {f2846afd-96d5-aad4-049a-0816b1462604-at-gmail.com} } 10, 53 -- Date: Tue, 5 Dec 2017 12:10:48 -0600 } 10, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) } 10, 53 -- Gecko/20100101 Thunderbird/52.5.0 } 10, 53 -- MIME-Version: 1.0 } 10, 53 -- In-Reply-To: {201712051615.vB5GFOLG008536-at-microscopy.com} } 10, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 10, 53 -- Content-Language: en-US } 10, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
=========================================== Dr. Nestor J. Zaluzec Argonne National Laboratory Photon Sciences Division 9700 S. Cass Ave Bldg 212 / A-143 Argonne, Illinois 60439 USA Email: Zaluzec-at-aaem.amc.anl.gov
Tel: 530-NES-TORZ (530-637-8679) has Voice Mail Lab: 630-252-7901 Fax: 630-252-4798
Senior Scientist - Argonne National Laboratory Fellow of the Microscopy Society of America Senior Fellow the Computational Institute - University of Chicago E.P. Wigner Fellow - Oak Ridge National Laboratory Past President Microscopy Society of America Adjunct Professor of Physics - Northern Illinois University & the University of Illinois at Chicago Visiting Professor of Microscopy - Manchester University
I had this issue with our old CM-10 for either of two reasons: either somehow the temperature sensor on the side on the ODP was out of position, or the cooling water line was clogged, and didn’t have sufficient flow. The sensor got jogged out of position once during maintenance, so that it wasn’t making proper contact with the pump and read wrong. This shut down the diffusion pump.
The clogged cooling line was commonplace, and easily fixed by running a bottle of CLR (Calcium Lime Rust), a US brand of scale remover, through the lines. The line could also be cleaned with a liter of 3% H2O2 or 100mL of 30% H2O2. In our case, the TEM was on a Haskris chiller, so I just added the solution to the chiller’s water tank and let the cooling water circulate for 4 hours - overnight (depending on how bad things were), then several changes of water until crud quit coming out of the cooling line.
If you’re on city water and not a cooler … hm … try removing the in-line filter from the housing and adding the H2O2 to the filter housing. This worked on an old ISI EM — except at that lab, we had a problem with biofilm, not corrosion, and used bleach.
Note: the cooling water coming in gets split into 2 lines in the CM-10, one to the ODP and one to the electronics. If there is a line clog at or after the split, the pump can overheat without affecting the electronics, and vice-versa.
Phil ------------- Philip Oshel Imaging Facility Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 989 774-3576 office 989 774-7567 lab
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both pveril-at-uth.gr as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: pveril-at-uth.gr Name: Panagiotis Berillis
Organization: University of Thessaly
Title-Subject: [Filtered] CM10 ODP water message
Message: Hi all Today I tried to switch on our Philips CM10 TEM. After an hour the message "ODP water" appeared on the monitor. Reading the manual I figured out that the message was about high water temperature (} 60 oC)on the ODP cooling system. There is the S30 switch that checks that temperature. Our water cooling system seems to work fine (temperature about 15oC). Any ideas how to fix the problem?
First, are you removing the meninges before embedding the brains? They can interfere with the agarose.
Second, this may/may not help — I embedded mormyrid brains, not vertebrate. But. Try 18% (wt:vol) 100 — 175 bloom gelatin instead of agarose. Liquify the gelatin and place tissue in the gelatin in a 55-60 deg C oven for 1.5 — 2 hours. Remove from oven, blot, place in fresh gelatin & put in oven for 15 — 30 minutes, remove from oven, blot, embed in fresh gelatin but do not put back in oven. Let gelatin set completely.
Mormyrids have a ginormous cerebellum that sits over the cerebrum and causes issues with the embedding medium getting to the rest of the brain. This gelatin method worked for them, so it should work for chick brains. I hope.
Phil ------------- Philip Oshel Imaging Facility Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 989 774-3576 office 989 774-7567 lab
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both driscollg2-at-winthrop.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Embedding Chick Brain (E7-E10) for Vibratome Sectioning
Message: I am doing brain electroporations with an RCASB plasmid and I wanted to visualize how efficient my electroporations were. Currently, I am embedding my heads in 3% agarose at 37 degrees between 15-20 minutes, cutting the block fairly close to the brain, and a final embedding in 6% agarose at 4 degrees for at least 20 minutes. The smallest sections I have been able to slice is 300 microns; however, I would like to get down to 200 microns. When I have attempted to go below 300 microns, the tissue gets destroyed, which I can clearly see that it's lacking structural support from how I am embedding. My question: What would be a more efficient way to embed my samples so I can section between 100-200 microns?
From shkrsugi29udma-at-gmail.com Wed Dec 6 22:56:32 2017 Return-Path: {shkrsugi29udma-at-gmail.com} Received: from gmail.com ([117.34.71.33]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id vB74uUkp023628 for {microscopylistserverarchive6-at-microscopy.com} ; Wed, 6 Dec 2017 22:56:31 -0600 Received: from rsmail.alkoholic.net ([93.213.168.168]) by external.newsubdomain.com with QMQP; Thu, 07 Dec 2017 08:43:46 +0500 Received: from mtu23.bigping.com ([Thu, 07 Dec 2017 08:33:49 +0500]) by mail.gimmicc.net with NNFMP; Thu, 07 Dec 2017 08:33:49 +0500 Message-ID: {2E6D6B4D.EE0481C1-at-gmail.com}
X-from: mtatham-at-ucmerced.edu
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mtatham-at-ucmerced.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jhyun-at-gatan.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: jhyun-at-gatan.com Name: John Hyun
Organization: Gatan
Title-Subject: [Filtered] EELS Training School April 2018, Pleasanton, CA USA
Message: EELS & EFTEM Analysis Training School - April 2018
Electron energy loss spectroscopy (EELS) is a powerful technique that provides both compositional and chemical information from sub-nanometer areas in the sample. As a course attendee, you will learn best practices to set up and optimize your EELS hardware and experimental protocols so you can capture and extract the maximum amount of compositional and chemical information from your TEM samples. Topics include:
Fundamentals of EELS and energy-filtered imaging in TEM Principles of operation of EFTEM and EELS systems Optimization of EFTEM and EELS data acquisition Quantification of elemental composition Other information provided by EFTEM/EELS and how best to extract it Use of EELS signals to form maps of elemental and chemical composition EFTEM and STEM EELS spectrum imaging techniques Identification of material phases via EELS fine structure mapping Applications to biological and physical science specimens
Login Host: 50.58.128.11 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
==============================Original Headers============================== 14, 53 -- From microscopy.listserver-at-gmail.com Thu Dec 7 07:01:38 2017 14, 53 -- Received: from mail-it0-f68.google.com (mail-it0-f68.google.com [209.85.214.68]) 14, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id vB7D1ckI014791 14, 53 -- for {microscopy-at-microscopy.com} ; Thu, 7 Dec 2017 07:01:38 -0600 14, 53 -- Received: by mail-it0-f68.google.com with SMTP id d137so14128993itc.2 14, 53 -- for {microscopy-at-microscopy.com} ; Thu, 07 Dec 2017 04:59:16 -0800 (PST) 14, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 14, 53 -- d=gmail.com; s=20161025; 14, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version 14, 53 -- :in-reply-to:content-language:content-transfer-encoding; 14, 53 -- bh=Ca9rJgyAcq73lS+zHaFuhmW1SvIQZLGA7pTZPtK8Gv4=; 14, 53 -- b=t3NGMf3yCvk9XzuUckQ6eFoQUGMyaNQjx0DpJeVMwfv59wfxkPYZfOnIXq3o0ULzeo 14, 53 -- i0xhx6bIzGB9G/ZVSvZUlk0AHxrYsGitkRUU9UJD4ChsR9qXYGNd1Y9584qHiObGma55 14, 53 -- gIiNmUAM9u4boOPfFmF2VbQ3hHnjit8aOV/g0l+Io6AZtTi0b2eqftZW+5jgh6sLV4jL 14, 53 -- GrJCOU01Nhi0oIePOvAk73KCD82w8DjyAYxzHLfdtV87leQ6XPDdMw6IjGuC9xoOJGfu 14, 53 -- 99v4V08rqYe/h/ifEJeejBy1UuHI4zfcLsUDBJ8H8ne1FdqaGkv+bfb8exgizzM6ZAdX 14, 53 -- qpXg== 14, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 14, 53 -- d=1e100.net; s=20161025; 14, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date 14, 53 -- :user-agent:mime-version:in-reply-to:content-language 14, 53 -- :content-transfer-encoding; 14, 53 -- bh=Ca9rJgyAcq73lS+zHaFuhmW1SvIQZLGA7pTZPtK8Gv4=; 14, 53 -- b=pDNLJHfgcDC10xSX1cNia8gxyfOC6rlQXx/E8uzQqAbznkvYoPDT5u8CDZWMCD9l8U 14, 53 -- Hp9JXXrp3f5cbm6qifEtjKn2521yS7972EOS8eP9L4QiWX16YIrpua5tKwy3DA/dvpHX 14, 53 -- zlCcMFaZLtuNaETsXu4US3pJpum5jzKhygdt4rET4hhTj9ygD0eoSgtVwQYBXeZL9oEb 14, 53 -- oMBrtQMJnhfghyPPx1W6ffispXNEIxO6Ey2zFOgiA2AXZSq6KWh0cF+Txyo9O+ignvMn 14, 53 -- Cbgw3YsMJSP5/ve7+jL4Loiaa6cGIDVnfX69/E6sTvxinw9tNzaeKocowXJNmseK9InV 14, 53 -- kgZg== 14, 53 -- X-Gm-Message-State: AKGB3mKVGb3ZJP7KspOfTh+3xQhp70r7EtFGI75C1nXN9wxAqcDVYIfi 14, 53 -- u0cq5roKReZgWBEwps3DBNWTXgDh 14, 53 -- X-Google-Smtp-Source: AGs4zMZaCZIyd9yQ3Y7o+J++fPbkZ0/wcVZRwMdHZAYYCfTIfykCt/QCKCIQU9ZMbx2vQsG5/xJm0Q== 14, 53 -- X-Received: by 10.36.181.80 with SMTP id j16mr1375444iti.118.1512651556032; 14, 53 -- Thu, 07 Dec 2017 04:59:16 -0800 (PST) 14, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:a5a6:99a0:ba92:c848]) 14, 53 -- by smtp.googlemail.com with ESMTPSA id w75sm2416639iod.66.2017.12.07.04.59.15 14, 53 -- for {microscopy-at-microscopy.com} 14, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 14, 53 -- Thu, 07 Dec 2017 04:59:15 -0800 (PST) 14, 53 -- Subject: viaWWW:EELS Training School April 2018, Pleasanton, CA USA 14, 53 -- References: {201712062254.vB6MsMXH013984-at-microscopy.com} 14, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} 14, 53 -- X-Forwarded-Message-Id: {201712062254.vB6MsMXH013984-at-microscopy.com} 14, 53 -- Message-ID: {986b69ee-7d87-1d99-bcb0-3ae38702f60f-at-gmail.com} 14, 53 -- Date: Thu, 7 Dec 2017 06:59:14 -0600 14, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) 14, 53 -- Gecko/20100101 Thunderbird/52.5.0 14, 53 -- MIME-Version: 1.0 14, 53 -- In-Reply-To: {201712062254.vB6MsMXH013984-at-microscopy.com} 14, 53 -- Content-Type: text/plain; charset=UTF-8; format=flowed 14, 53 -- Content-Language: en-US 14, 53 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
From davishal182bomv-at-gmail.com Sat Dec 9 11:31:36 2017 Return-Path: {davishal182bomv-at-gmail.com} Received: from gmail.com (hn.kd.pix [219.155.154.245] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id vB9HVP5R005852 for {microscopylistserverarchive6-at-microscopy.com} ; Sat, 9 Dec 2017 11:31:35 -0600 Received: from relay37.vosimerkam.net ([Sat, 09 Dec 2017 12:18:14 -0500]) by asx121.turbo-inline.com with NNFMP; Sat, 09 Dec 2017 12:18:14 -0500 Received: from smtp.doneohx.com ([Sat, 09 Dec 2017 12:14:06 -0500]) by smtp-server1.cfdenselr.com with NNFMP; Sat, 09 Dec 2017 12:14:06 -0500 Received: from rly04.hottestmile.com ([Sat, 09 Dec 2017 12:14:01 -0500]) by smtp.doneohx.com with NNFMP; Sat, 09 Dec 2017 12:14:01 -0500 Message-ID: {B4426A94.52A573BF-at-gmail.com}
X-from: michelle.plue-at-duke.edu
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both michelle.plue-at-duke.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Message: Duke Universitys Share Materials Instrumentation Facility (SMiF, http://smif.pratt.duke.edu/ ) is looking for a Cryo Electron Microscopist. The applicant should have at least 2 years of biological or cryo TEM experience. Experience operating a Krios or Artica system would be ideal. A minimum of a BS degree in a biological science or engineering is required. Good interpersonal and communication skills are essential. This is a full time, permanent position. For more information please see the posting - https://sjobs.brassring.com/TGnewUI/Search/Home/Home?partnerid=25017&siteid=5172#jobDetails=1217139_5172
Cheers, Michelle
Login Host: 152.3.34.5 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
==============================Original Headers============================== 10, 53 -- From microscopy.listserver-at-gmail.com Sun Dec 10 10:06:24 2017 10, 53 -- Received: from mail-it0-f68.google.com (mail-it0-f68.google.com [209.85.214.68]) 10, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id vBAG6NeY009681 10, 53 -- for {microscopy-at-microscopy.com} ; Sun, 10 Dec 2017 10:06:24 -0600 10, 53 -- Received: by mail-it0-f68.google.com with SMTP id z6so10700625iti.4 10, 53 -- for {microscopy-at-microscopy.com} ; Sun, 10 Dec 2017 08:04:12 -0800 (PST) 10, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 10, 53 -- d=gmail.com; s=20161025; 10, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version 10, 53 -- :in-reply-to:content-language:content-transfer-encoding; 10, 53 -- bh=qdBAqAtIv3uo/U2r30PsgU93MU8NIK07bAr1gb99yds=; 10, 53 -- b=QOJ5DMoaQKUsj14aA8BSd5SHAwk9ta7Nw8T2/trAuov70tXLvsEXuG6p8rW1OoBJjA 10, 53 -- DUMLfF/GuQxOkXuqWbVA0mOmqp+DEaWhwpl/ybs/dpXfTNGzBnzicXjsdVps4llIFcjG 10, 53 -- e9vHNa8bR3eUd5u1rFZab8u/UOMKtmOXOK+Mcfq4KBuFwzJe2w7qAXLdJljAOWNvgrN9 10, 53 -- JUBpvo/WD3BlXyZcXZc8l3rKY+bkNF2XZtS5bDWVmwHm+/urr1sim6WfED9C4FfRalUH 10, 53 -- x9r6VuWvX8iDY0skq6hJY/D3CcuqZFL2nZU2QcQLffMbgpRHHQsh6QRNRaZ6I8bFJ0bL 10, 53 -- URIA== 10, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 10, 53 -- d=1e100.net; s=20161025; 10, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date 10, 53 -- :user-agent:mime-version:in-reply-to:content-language 10, 53 -- :content-transfer-encoding; 10, 53 -- bh=qdBAqAtIv3uo/U2r30PsgU93MU8NIK07bAr1gb99yds=; 10, 53 -- b=nRW/nQVG2YqDv13lb2bikUr4g5whg5VNrMGe4bTUqEA8EFPnoJWiKyp8hje+hCL+pC 10, 53 -- cC0qpw1OWhyhnukYC9ro+vv+K5L+LDAoG0DX+5jH7HExS8hKkZVyGAd3b0niDsqwVn9c 10, 53 -- reuFvK3yY2ckBQyT/aldtjvlBKG/RnDVsG5+bLDC0wdn67sJnCgE+YACbN00WOcbYuXq 10, 53 -- ooJ1x2RolxgjDkoMWQj0pNRRfjXePiFAt0dt1+U8oJVgoPjHP705oJjYN9qsznEOXdua 10, 53 -- ZjUK2aWs4xxq4rxnfIWWc5KS2Dr/34RBTUIRdm1V219Dz4P071HXW4VnPi6OLr+PuLLH 10, 53 -- 0A3A== 10, 53 -- X-Gm-Message-State: AKGB3mLlwWzKrZQfzZmTRloVYRy2Jg8qhoFSLSAlljUTVeTkPTg5ZFcR 10, 53 -- eF2l3EHOJTKhxpeCU3pCsIdP8MRF 10, 53 -- X-Google-Smtp-Source: ACJfBov/e/XdSN11gPRVa2OqMQNBh+6tDqofQNxrMAJOsgQbwqFPYU9C7xvQFTobPhzrwx9CwqOlHg== 10, 53 -- X-Received: by 10.36.239.195 with SMTP id i186mr6979241ith.29.1512921851523; 10, 53 -- Sun, 10 Dec 2017 08:04:11 -0800 (PST) 10, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:1c1:5eab:3890:173f]) 10, 53 -- by smtp.googlemail.com with ESMTPSA id i62sm5446496ioe.50.2017.12.10.08.04.10 10, 53 -- for {microscopy-at-microscopy.com} 10, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 10, 53 -- Sun, 10 Dec 2017 08:04:10 -0800 (PST) 10, 53 -- Subject: viaWWW:Cryo-EM Position Open at Duke 10, 53 -- References: {201712071841.vB7IfhCo010829-at-microscopy.com} 10, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 10, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} 10, 53 -- X-Forwarded-Message-Id: {201712071841.vB7IfhCo010829-at-microscopy.com} 10, 53 -- Message-ID: {3de56be0-0806-b9ef-5267-afd9fe71c2ba-at-gmail.com} 10, 53 -- Date: Sun, 10 Dec 2017 10:04:08 -0600 10, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) 10, 53 -- Gecko/20100101 Thunderbird/52.5.0 10, 53 -- MIME-Version: 1.0 10, 53 -- In-Reply-To: {201712071841.vB7IfhCo010829-at-microscopy.com} 10, 53 -- Content-Type: text/plain; charset=UTF-8; format=flowed 10, 53 -- Content-Language: en-US 10, 53 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Dear Garrett, There are some missing details. What is your fixative or is this live? Are these expressing a fluorescent protein or are you planning on immunolabeling? what instrument are you using to section and how are you attaching the sample to the sample holder.
I’ve found that embryonic avian brain requires slow advance speed and high amplitude, whether live or fixed. It is also very sensitive to knife angle. How are you attaching to the specimen holder? Not all cyanoacryates work with wet samples. Iv’e generally had the the best results orienting the tissue with the ventral surface facing the knife. there is a tendency for the sectioning shear force to push the brain apart when cut from the dorsal surface. As Phil mentions, surrounding membranes can cause shear forces that displace the sample. Or they drape around the razor’s edge and get dragged through the tissue. I’ve cut live embryonic brain down to 200 um by gluing it to the holder and just using an agarose block to “backstop” the brain. Such a backstop can also help with any fragile tissue that does not offer much internal strength. In another project, embryonic avian brain was sectioned after blocking the tissue to give a flat base and gluing to the stub. Then low melting point agarose was ladled over it and allowed to harden. Another approach for small floppy tissues, like early embryonic brain or with spinal cord, is to cut the rounded end from a gelatin capsule or BEEM capsule and use the capsule as a mold for embedding with agarose. If embedded with the cap in place, remove the cap to expose the tissue. Or trim down the upper end to expose the tissue for slicing. Regards Glen MacDonald depts.washington.edu/digmicro glenmac-at-uw.edu
} On Dec 5, 2017, at 10:16 AM, microscopy.listserver-at-gmail.com wrote: } } Email: driscollg2-at-winthrop.edu Name: Garrett Driscoll } } Organization: Winthrop University } } Title-Subject: [Filtered] Embedding Chick Brain (E7-E10) for Vibratome Sectioning } } Message: I am doing brain electroporations with an RCASB plasmid and I wanted to visualize how } efficient my electroporations were. Currently, I am embedding my heads in 3% agarose at 37 degrees } between 15-20 minutes, cutting the block fairly close to the brain, and a final embedding in 6% } agarose at 4 degrees for at least 20 minutes. The smallest sections I have been able to slice is 300 } microns; however, I would like to get down to 200 microns. When I have attempted to go below 300 } microns, the tissue gets destroyed, which I can clearly see that it's lacking structural support } from how I am embedding. } My question: What would be a more efficient way to embed my samples so I can section between 100-200 } microns? }
Hi, you can try Celloidin embedding media, with that you can section as thin as 15 micron I had used it for embedding Golgi stained human and mouse brain tissue.
here is the link for detailed description hope it helps you
Arvind Singh Pundir National Brain Research Centre, Manesar, Gurgaon Haryana- INDIA
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
X-from: driscollg2-at-winthrop.edu
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both driscollg2-at-winthrop.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Embedding Chick Brain (E7-E10) for Vibratome Sectioning
Message: I am doing brain electroporations with an RCASB plasmid and I wanted to visualize how efficient my electroporations were. Currently, I am embedding my heads in 3% agarose at 37 degrees between 15-20 minutes, cutting the block fairly close to the brain, and a final embedding in 6% agarose at 4 degrees for at least 20 minutes. The smallest sections I have been able to slice is 300 microns; however, I would like to get down to 200 microns. When I have attempted to go below 300 microns, the tissue gets destroyed, which I can clearly see that it's lacking structural support from how I am embedding. My question: What would be a more efficient way to embed my samples so I can section between 100-200 microns?
Login Host: 206.74.212.199 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
X-from: Reuschle, David (D) {DReuschle-at-dow.com}
Email: dreuschle-at-dow.com {mailto:dreuschle-at-dow.com} Name: David Reuschle
Organization: Dow Chemical
Title-Subject: Inert Sample Transfer for SEM
Message: I would like to get some advice/input from folks that have worked with air sensitive and moisture sensitive samples for SEM-EDS. What are some of the methods and best practices to get the sample from the glovebox into the SEM without exposing, and thus modifying, the sample? Thanks in advance!
==============================Original Headers============================== 6, 53 -- From microscopy.listserver-at-gmail.com Sun Dec 10 10:19:48 2017 6, 53 -- Received: from mail-it0-f54.google.com (mail-it0-f54.google.com [209.85.214.54]) 6, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id vBAGJmS4021027 6, 53 -- for {microscopy-at-microscopy.com} ; Sun, 10 Dec 2017 10:19:48 -0600 6, 53 -- Received: by mail-it0-f54.google.com with SMTP id f143so10642407itb.0 6, 53 -- for {microscopy-at-microscopy.com} ; Sun, 10 Dec 2017 08:17:36 -0800 (PST) 6, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 53 -- d=gmail.com; s=20161025; 6, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version 6, 53 -- :in-reply-to:content-language:content-transfer-encoding; 6, 53 -- bh=Vm6Q3zkxjOG2wutfLFkienPL6/RQQZI6Tctta1yRJCc=; 6, 53 -- b=BmiK1UyH+LkL2dMEsQ2WRjXGODFuVpEwn1fMtX+9BS+w+XEdPa6flsPeQcsMeszzsa 6, 53 -- nl2/UPvsFwy6PVYKIFXJE84b3QoP+P2OGvu20pEDXj2agbfUhVvYEel62pI73jAS/MLS 6, 53 -- uNHE7YeNf96D5NPFiRcy8q7zwzuwoIZuFjubMVfkAzoh5emUZ65EgZ1FADxDFXox4cb9 6, 53 -- Bz1MmlWPmdXN/MzJhU+Y31luwsSglk1N+c+oqvn1iGqGDkvr/vn7QCoRHy2Nk2SAtsYp 6, 53 -- gsKCn/SnEqqg254a7o6fJlKdjmyHa6HmKPzWMVeQStfQEj/mWc74NFOB2DhM78Fe52/J 6, 53 -- a5kg== 6, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 53 -- d=1e100.net; s=20161025; 6, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date 6, 53 -- :user-agent:mime-version:in-reply-to:content-language 6, 53 -- :content-transfer-encoding; 6, 53 -- bh=Vm6Q3zkxjOG2wutfLFkienPL6/RQQZI6Tctta1yRJCc=; 6, 53 -- b=R3Iyjl7cjffXExzdOU3c6UmLAWKpp7Fza3VQ2BYtzT/V/NDxHmC8B3Z2+hgYjlCZC5 6, 53 -- iTkNXfTLw3jLRa56+nEIlxDnjWL8WtLx7C2bJZ8eqi+AdaJsqalWE1UNF0x/Hw0CIIb0 6, 53 -- Z82g1sfq4LGfM18XtnBPPXX/FeS4l5la4RCwc+KA+Ms+hsO542tDc8ePikx9vwFtmBd4 6, 53 -- kOfLSrw8PZByns2J/dUO5fRS6Ig4jV3bC7h2D5xYAl0JH8qjl+3w2EpTeCEHj+AdqDWB 6, 53 -- UsN167Vk9Rzk2P1qD74kcejpNMDvNIL1zUOyvvPhRjv12gCNZfVQ/mNMkrxVKscN0BpB 6, 53 -- oYTw== 6, 53 -- X-Gm-Message-State: AKGB3mKmuLMDR0bJ6+NYo/VraRaAp4wEiIkTIJWEZ6H6cz7tTvA9gzzD 6, 53 -- vsKxsGgF0jfEkLvFwrW5RkIuK+Cv 6, 53 -- X-Google-Smtp-Source: AGs4zMbXYKhEkKNT8YcNmIkJBOqA0cwDWrezPlDBSAyDi80kpy+4bmSHBL7qFiOqVtJ96A8ff4DmvA== 6, 53 -- X-Received: by 10.36.222.68 with SMTP id d65mr15382648itg.67.1512922655881; 6, 53 -- Sun, 10 Dec 2017 08:17:35 -0800 (PST) 6, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:d9cd:838a:961c:491]) 6, 53 -- by smtp.googlemail.com with ESMTPSA id i203sm2842682itb.40.2017.12.10.08.17.35 6, 53 -- for {microscopy-at-microscopy.com} 6, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 6, 53 -- Sun, 10 Dec 2017 08:17:35 -0800 (PST) 6, 53 -- Subject: Inert Sample Transfer for SEM 6, 53 -- References: {BN6P164MB006875F5F92452F74499B287BE330-at-BN6P164MB0068.NAMP164.PROD.OUTLOOK.COM} 6, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 6, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} 6, 53 -- X-Forwarded-Message-Id: {BN6P164MB006875F5F92452F74499B287BE330-at-BN6P164MB0068.NAMP164.PROD.OUTLOOK.COM} 6, 53 -- Message-ID: {ad3a2c56-4fd7-3aed-c96d-937382251936-at-gmail.com} 6, 53 -- Date: Sun, 10 Dec 2017 10:17:35 -0600 6, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) 6, 53 -- Gecko/20100101 Thunderbird/52.5.0 6, 53 -- MIME-Version: 1.0 6, 53 -- In-Reply-To: {BN6P164MB006875F5F92452F74499B287BE330-at-BN6P164MB0068.NAMP164.PROD.OUTLOOK.COM} 6, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed 6, 53 -- Content-Language: en-US 6, 53 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
X-from: Calabrese, Michael {Michael.Calabrese-at-Teledyne.com} Is there adequate water flow in the DP site glass? Are the top of the cooling coils on the DP cool to the touch? Occasionally the reducer in the water line gets clogged with debris.
Mike
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Tuesday, December 05, 2017 10:48 AM To: Calabrese, Michael
X-from: pveril-at-uth.gr
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both pveril-at-uth.gr as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: pveril-at-uth.gr Name: Panagiotis Berillis
Organization: University of Thessaly
Title-Subject: [Filtered] CM10 ODP water message
Message: Hi all Today I tried to switch on our Philips CM10 TEM. After an hour the message "ODP water" appeared on the monitor. Reading the manual I figured out that the message was about high water temperature (} 60 oC)on the ODP cooling system. There is the S30 switch that checks that temperature. Our water cooling system seems to work fine (temperature about 15oC). Any ideas how to fix the problem?
Panagiotis
Login Host: 109.242.105.72 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
We got our EDAX system fixed. At the end we needed to replace the data processing board. Fortunately we had one available in out lab from another EDAX system.
Thanks for the suggestions anyway
Regards,
Erico Freitas Físico/Physicist Centro de Microscopia Universidade Federal de Minas Gerais
Coordenador do Lab. de Microscopia Eletrônica dr Transmissão
On Nov 21, 2017 12:45 AM, {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote:
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver On-Line {http://www.microscopy.com/MicroscopyListserver On-Line} Help http://www.microscopy.com/MicroscopyListserver/FAQ.html {http://www.microscopy.com/MicroscopyListserver/FAQ.html} ----------------------------------------------------------------------------
X-from: Ferenc Molnar {ferenc.l.molnar-at-googlemail.com {mailto:ferenc.l.molnar-at-googlemail.com} }
Dear Erico,
this sounds to me as there is an issue with additional light in the chamber. Make sure that all light sources - especially chamber scope - are turned off. A little bit tricky might be a touch alarm: on some SEM (do we talk about SEM?) the stage alarm triggers some safety light barrier in order to prevent another collision. To turn these lights off, you have to initialize the stage.
If you can exclude additional light in the chamber, there is maybe an issue with the detector: it can be the cooling system or some issue with the preamp of the SDD/SiLi.
I hope that helps. In general, EDS detectors are very sensible to all kind of light, not only the Xray you want to see.
With best regards, Ferenc
{microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} {mailto:microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } } schrieb am So., 19. Nov. 2017 um 16:38 Uhr:
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver {http://www.microscopy.com/MicroscopyListserver} On-Line {http://www.microscopy.com/MicroscopyListserverOn-Line {http://www.microscopy.com/MicroscopyListserverOn-Line} } Help http://www.microscopy.com/MicroscopyListserver/FAQ.html {http://www.microscopy.com/MicroscopyListserver/FAQ.html} ----------------------------------------------------------------------------
Our EDS is showing a high intensify peak at about 0.2 keV even with beam on vacuum. We're not getting C signal anymore and the energy resolution is getting worse. There'is been an energy shift also. The issue remains after run an automatic callibraion. Does anybody know what more could be done? Or is it a sign that our detector is almost dead?
Regards,
Erico Freitas Físico/Physicist Centro de Microscopia Universidade Federal de Minas Gerais
Coordenador do Lab. de Microscopia Eletrônica dr Transmissão
Have you fixed the tissue in paraformaldehyde? Karen Bentley
Karen Bentley, M.S. Director Electron Microscope Shared Resource Laboratory Pathology & Laboratory Medicine University of Rochester Medical Center 575 Elmwood Avenue Rochester, NY 14642 585-275-1954
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Tuesday, December 05, 2017 1:23 PM To: Bentley, Karen {Karen_Bentley-at-URMC.Rochester.edu}
X-from: John Nailon {jvnailon-at-gmail.com}
Hi Sylvian,
Every time you use the evaporator to coat a sample with carbon or metal everything gets coated i eluding the inside of the glass bell jar, all of the electrodes and all of the insulated connections. If you do not clean the system periodically then you will start to have slow pump down times, inability to see inside the bell jar, and potential short circuits across the insulated connections within the bell jar.
Regards John V Nailon Retired Electron Microscopist
John V Nailon Mob: 0423 020 680 Email: jvnailon-at-gmail.com {mailto:jvnailon-at-gmail.com}
On Tue, Dec 5, 2017 at 3:22 AM, {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote:
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver On-Line {http://www.microscopy.com/MicroscopyListserver On-Line} Help http://www.microscopy.com/MicroscopyListserver/FAQ.html {http://www.microscopy.com/MicroscopyListserver/FAQ.html} ----------------------------------------------------------------------------
X-from: Ribardire Michel {m.ribardiere-at-jeol.fr}
Panagiotis,
It could .be the sensor itself problem, but mostly Can come from the water Flow , or pressure too low To get the necessary Flow rate Regards Michel
Envoy depuis mon appareil mobile Samsung.
-------- Message d'origine -------- De : microscopy.listserver-at-gmail.com Date : 05/12/2017 19:11 (GMT+01:00) : Ribardire Michel {m.ribardiere-at-jeol.fr} Objet : [Microscopy] viaWWW:
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
X-from: pveril-at-uth.gr
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both pveril-at-uth.gr as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: pveril-at-uth.gr Name: Panagiotis Berillis
Organization: University of Thessaly
Title-Subject: [Filtered] CM10 ODP water message
Message: Hi all Today I tried to switch on our Philips CM10 TEM. After an hour the message "ODP water" appeared on the monitor. Reading the manual I figured out that the message was about high water temperature (} 60 oC)on the ODP cooling system. There is the S30 switch that checks that temperature. Our water cooling system seems to work fine (temperature about 15oC). Any ideas how to fix the problem?
Panagiotis
Login Host: 109.242.105.72 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
I have designed specimen rods that protect specimens from air during transfer between a glove box and the microscope. If I can be of help let me know.
Wil Bigelow
-- Wilbur C. Bigelow, PhD, LCDR USNR (Ret) Professor Emeritus of Materials Engineering The University of Michigan 2911 Whittier Court, Ann Arbor MI 48104
==============================Original Headers============================== 4, 47 -- From bigelow-at-umich.edu Sun Dec 10 22:07:30 2017 4, 47 -- Received: from mail-it0-f68.google.com (mail-it0-f68.google.com [209.85.214.68]) 4, 47 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id vBB47Uxh006296 4, 47 -- for {microscopy-at-microscopy.com} ; Sun, 10 Dec 2017 22:07:30 -0600 4, 47 -- Received: by mail-it0-f68.google.com with SMTP id x28so12870595ita.0 4, 47 -- for {microscopy-at-microscopy.com} ; Sun, 10 Dec 2017 20:05:20 -0800 (PST) 4, 47 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 47 -- d=umich.edu; s=google-2016-06-03; 4, 47 -- h=message-id:date:from:user-agent:mime-version:to:subject 4, 47 -- :content-transfer-encoding; 4, 47 -- bh=UQJNuKJizndcAVcp5LYB8POxnTPPRr7zprGCEZrOv+w=; 4, 47 -- b=jtmInwKPVBH4kGfwob+r3wsRyR3MatsJ4E4um6BMZkMYNQJTnKydPv5ADfDxxqyTsp 4, 47 -- Mb2HslBWcdvlzi40wrYcoRgTbcSO0sEKmmHw4S1O9pceOUoa+7iDuwHQkjv0+jRB1uv3 4, 47 -- IfNqvtgmMDYgt1ISA5yxWwdOO+Yn0lfHgbScLDjikdfcqOSi3h1s0InBdyxdHpiNbOF+ 4, 47 -- koDwFmLEUuPf0G69moxRtRHgaDxFthU0TKxl4Sx2geb8zQ1aMeb2Z4twxZhJvHN03srd 4, 47 -- T+j56r7opzb1wZtb/2Xu0SK6AlmFtTQcEqujaWOQ4kVj9fXyqwX9HG2esHAfp1GySojH 4, 47 -- ahXQ== 4, 47 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 47 -- d=1e100.net; s=20161025; 4, 47 -- h=x-gm-message-state:message-id:date:from:user-agent:mime-version:to 4, 47 -- :subject:content-transfer-encoding; 4, 47 -- bh=UQJNuKJizndcAVcp5LYB8POxnTPPRr7zprGCEZrOv+w=; 4, 47 -- b=McZVT6KPHpEun7z4BJaHHMneUP29lWcKXPQhEIVLeb8kOs6z283GCAa92SpOitQ2Ou 4, 47 -- P1RJNOgvIFlEKdE7zC+8URGLL6ulHZUw+Cmvx6mlDYjScy5uG6Hsnre/8EXPUZPY2yXH 4, 47 -- vGvgw6CKje5lV/MT+vGShx56WEmq7JV2NgmstAeTo9kR6B5Mur+Zwp5P4W4n0lYnVxb8 4, 47 -- 5gZ82Y7Xv2KcxK2aB4TAWk8a9JQIr2NOL3pqWZ/qClbEyi9odfbPe3LlMlFJrRPGdKvd 4, 47 -- 1Tryvc8VwjFqucLRKzv9qNDc+C4P4JwaPGhzzi6hUIRDpSNxb8VwaKC0ZksWjiDGhJ+s 4, 47 -- JbtA== 4, 47 -- X-Gm-Message-State: AKGB3mKMDtX2ZoFluX0jOx7Ms5dQ9WzrvmSsRZskh4oL+fugnzZ28rxr 4, 47 -- pBdtFCXPS/+P8PodcOiZG3Xfl1ZK 4, 47 -- X-Google-Smtp-Source: AGs4zMacv6xPCbctOvRKqWuGxFjcwEe6FtmFPO+HUERwPu9DBy7PVn2nRzvVAtH+RSz+atMTXRdrMg== 4, 47 -- X-Received: by 10.36.0.73 with SMTP id 70mr17091799ita.8.1512965119470; 4, 47 -- Sun, 10 Dec 2017 20:05:19 -0800 (PST) 4, 47 -- Received: from ?IPv6:2602:306:3b93:aba0:230:65ff:fe6b:155c? ([2602:306:3b93:aba0:230:65ff:fe6b:155c]) 4, 47 -- by smtp.gmail.com with ESMTPSA id q6sm3572889ita.38.2017.12.10.20.05.18 4, 47 -- for {microscopy-at-microscopy.com} 4, 47 -- (version=TLS1 cipher=ECDHE-RSA-AES128-SHA bits=128/128); 4, 47 -- Sun, 10 Dec 2017 20:05:18 -0800 (PST) 4, 47 -- Message-ID: {5A2E03FC.20105-at-umich.edu} 4, 47 -- Date: Sun, 10 Dec 2017 23:05:16 -0500 4, 47 -- From: Wil Bigelow {bigelow-at-umich.edu} 4, 47 -- User-Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X 10.4; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 4, 47 -- MIME-Version: 1.0 4, 47 -- To: microscopy-at-microscopy.com 4, 47 -- Subject: Re: Specimen transfer rod 4, 47 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 47 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
X-from: bigelow-at-umich.edu ---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
I have designed specimen rods that protect specimens from air during transfer between a glove box and the microscope. If I can be of help let me know.
Wil Bigelow
-- Wilbur C. Bigelow, PhD, LCDR USNR (Ret) Professor Emeritus of Materials Engineering The University of Michigan 2911 Whittier Court, Ann Arbor MI 48104
==============================Original Headers============================== 4, 47 -- From bigelow-at-umich.edu Sun Dec 10 22:07:30 2017 4, 47 -- Received: from mail-it0-f68.google.com (mail-it0-f68.google.com [209.85.214.68]) 4, 47 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id vBB47Uxh006296 4, 47 -- for {microscopy-at-microscopy.com} ; Sun, 10 Dec 2017 22:07:30 -0600 4, 47 -- Received: by mail-it0-f68.google.com with SMTP id x28so12870595ita.0 4, 47 -- for {microscopy-at-microscopy.com} ; Sun, 10 Dec 2017 20:05:20 -0800 (PST) 4, 47 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 47 -- d=umich.edu; s=google-2016-06-03; 4, 47 -- h=message-id:date:from:user-agent:mime-version:to:subject 4, 47 -- :content-transfer-encoding; 4, 47 -- bh=UQJNuKJizndcAVcp5LYB8POxnTPPRr7zprGCEZrOv+w=; 4, 47 -- b=jtmInwKPVBH4kGfwob+r3wsRyR3MatsJ4E4um6BMZkMYNQJTnKydPv5ADfDxxqyTsp 4, 47 -- Mb2HslBWcdvlzi40wrYcoRgTbcSO0sEKmmHw4S1O9pceOUoa+7iDuwHQkjv0+jRB1uv3 4, 47 -- IfNqvtgmMDYgt1ISA5yxWwdOO+Yn0lfHgbScLDjikdfcqOSi3h1s0InBdyxdHpiNbOF+ 4, 47 -- koDwFmLEUuPf0G69moxRtRHgaDxFthU0TKxl4Sx2geb8zQ1aMeb2Z4twxZhJvHN03srd 4, 47 -- T+j56r7opzb1wZtb/2Xu0SK6AlmFtTQcEqujaWOQ4kVj9fXyqwX9HG2esHAfp1GySojH 4, 47 -- ahXQ== 4, 47 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 47 -- d=1e100.net; s=20161025; 4, 47 -- h=x-gm-message-state:message-id:date:from:user-agent:mime-version:to 4, 47 -- :subject:content-transfer-encoding; 4, 47 -- bh=UQJNuKJizndcAVcp5LYB8POxnTPPRr7zprGCEZrOv+w=; 4, 47 -- b=McZVT6KPHpEun7z4BJaHHMneUP29lWcKXPQhEIVLeb8kOs6z283GCAa92SpOitQ2Ou 4, 47 -- P1RJNOgvIFlEKdE7zC+8URGLL6ulHZUw+Cmvx6mlDYjScy5uG6Hsnre/8EXPUZPY2yXH 4, 47 -- vGvgw6CKje5lV/MT+vGShx56WEmq7JV2NgmstAeTo9kR6B5Mur+Zwp5P4W4n0lYnVxb8 4, 47 -- 5gZ82Y7Xv2KcxK2aB4TAWk8a9JQIr2NOL3pqWZ/qClbEyi9odfbPe3LlMlFJrRPGdKvd 4, 47 -- 1Tryvc8VwjFqucLRKzv9qNDc+C4P4JwaPGhzzi6hUIRDpSNxb8VwaKC0ZksWjiDGhJ+s 4, 47 -- JbtA== 4, 47 -- X-Gm-Message-State: AKGB3mKMDtX2ZoFluX0jOx7Ms5dQ9WzrvmSsRZskh4oL+fugnzZ28rxr 4, 47 -- pBdtFCXPS/+P8PodcOiZG3Xfl1ZK 4, 47 -- X-Google-Smtp-Source: AGs4zMacv6xPCbctOvRKqWuGxFjcwEe6FtmFPO+HUERwPu9DBy7PVn2nRzvVAtH+RSz+atMTXRdrMg== 4, 47 -- X-Received: by 10.36.0.73 with SMTP id 70mr17091799ita.8.1512965119470; 4, 47 -- Sun, 10 Dec 2017 20:05:19 -0800 (PST) 4, 47 -- Received: from ?IPv6:2602:306:3b93:aba0:230:65ff:fe6b:155c? ([2602:306:3b93:aba0:230:65ff:fe6b:155c]) 4, 47 -- by smtp.gmail.com with ESMTPSA id q6sm3572889ita.38.2017.12.10.20.05.18 4, 47 -- for {microscopy-at-microscopy.com} 4, 47 -- (version=TLS1 cipher=ECDHE-RSA-AES128-SHA bits=128/128); 4, 47 -- Sun, 10 Dec 2017 20:05:18 -0800 (PST) 4, 47 -- Message-ID: {5A2E03FC.20105-at-umich.edu} 4, 47 -- Date: Sun, 10 Dec 2017 23:05:16 -0500 4, 47 -- From: Wil Bigelow {bigelow-at-umich.edu} 4, 47 -- User-Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X 10.4; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 4, 47 -- MIME-Version: 1.0 4, 47 -- To: microscopy-at-microscopy.com 4, 47 -- Subject: [Filtered] Re: Specimen transfer rod 4, 47 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 47 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
-------- Forwarded Message -------- X-from: Johnson, Bradley R {Bradley.Johnson-at-pnnl.gov}
Hello All, We do some work with Li-ion batteries. I built an airlock chamber for our SEM that I would purge with N2 or Ar; and then we used the South Bay Technologies Sample Savers to transfer the specimen from the glove box to the airlock chamber on the SEM. It was adequate.
-Brad
On 12/10/17, 9:01 AM, "microscopy.listserver-at-gmail.com" {microscopy.listserver-at-gmail.com} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Quantomix capsules from the EM suppliers might work. You can seal them in the glovebox and easily transport them to the SEM.
Rich
On 12/10/2017 11:41 AM, microscopy.listserver-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: Reuschle, David (D) {DReuschle-at-dow.com} } } Email: dreuschle-at-dow.com {mailto:dreuschle-at-dow.com} Name: David Reuschle } } Organization: Dow Chemical } } Title-Subject: Inert Sample Transfer for SEM } } Message: I would like to get some advice/input from folks that have worked with air sensitive and } moisture sensitive samples for SEM-EDS. What are some of the methods and best practices to get the } sample from the glovebox into the SEM without exposing, and thus modifying, the sample? Thanks in } advance! } } } ==============================Original Headers============================== } 6, 53 -- From microscopy.listserver-at-gmail.com Sun Dec 10 10:19:48 2017 } 6, 53 -- Received: from mail-it0-f54.google.com (mail-it0-f54.google.com [209.85.214.54]) } 6, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id vBAGJmS4021027 } 6, 53 -- for {microscopy-at-microscopy.com} ; Sun, 10 Dec 2017 10:19:48 -0600 } 6, 53 -- Received: by mail-it0-f54.google.com with SMTP id f143so10642407itb.0 } 6, 53 -- for {microscopy-at-microscopy.com} ; Sun, 10 Dec 2017 08:17:36 -0800 (PST) } 6, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 6, 53 -- d=gmail.com; s=20161025; } 6, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 6, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 6, 53 -- bh=Vm6Q3zkxjOG2wutfLFkienPL6/RQQZI6Tctta1yRJCc=; } 6, 53 -- b=BmiK1UyH+LkL2dMEsQ2WRjXGODFuVpEwn1fMtX+9BS+w+XEdPa6flsPeQcsMeszzsa } 6, 53 -- nl2/UPvsFwy6PVYKIFXJE84b3QoP+P2OGvu20pEDXj2agbfUhVvYEel62pI73jAS/MLS } 6, 53 -- uNHE7YeNf96D5NPFiRcy8q7zwzuwoIZuFjubMVfkAzoh5emUZ65EgZ1FADxDFXox4cb9 } 6, 53 -- Bz1MmlWPmdXN/MzJhU+Y31luwsSglk1N+c+oqvn1iGqGDkvr/vn7QCoRHy2Nk2SAtsYp } 6, 53 -- gsKCn/SnEqqg254a7o6fJlKdjmyHa6HmKPzWMVeQStfQEj/mWc74NFOB2DhM78Fe52/J } 6, 53 -- a5kg== } 6, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 6, 53 -- d=1e100.net; s=20161025; } 6, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 6, 53 -- :user-agent:mime-version:in-reply-to:content-language } 6, 53 -- :content-transfer-encoding; } 6, 53 -- bh=Vm6Q3zkxjOG2wutfLFkienPL6/RQQZI6Tctta1yRJCc=; } 6, 53 -- b=R3Iyjl7cjffXExzdOU3c6UmLAWKpp7Fza3VQ2BYtzT/V/NDxHmC8B3Z2+hgYjlCZC5 } 6, 53 -- iTkNXfTLw3jLRa56+nEIlxDnjWL8WtLx7C2bJZ8eqi+AdaJsqalWE1UNF0x/Hw0CIIb0 } 6, 53 -- Z82g1sfq4LGfM18XtnBPPXX/FeS4l5la4RCwc+KA+Ms+hsO542tDc8ePikx9vwFtmBd4 } 6, 53 -- kOfLSrw8PZByns2J/dUO5fRS6Ig4jV3bC7h2D5xYAl0JH8qjl+3w2EpTeCEHj+AdqDWB } 6, 53 -- UsN167Vk9Rzk2P1qD74kcejpNMDvNIL1zUOyvvPhRjv12gCNZfVQ/mNMkrxVKscN0BpB } 6, 53 -- oYTw== } 6, 53 -- X-Gm-Message-State: AKGB3mKmuLMDR0bJ6+NYo/VraRaAp4wEiIkTIJWEZ6H6cz7tTvA9gzzD } 6, 53 -- vsKxsGgF0jfEkLvFwrW5RkIuK+Cv } 6, 53 -- X-Google-Smtp-Source: AGs4zMbXYKhEkKNT8YcNmIkJBOqA0cwDWrezPlDBSAyDi80kpy+4bmSHBL7qFiOqVtJ96A8ff4DmvA== } 6, 53 -- X-Received: by 10.36.222.68 with SMTP id d65mr15382648itg.67.1512922655881; } 6, 53 -- Sun, 10 Dec 2017 08:17:35 -0800 (PST) } 6, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:d9cd:838a:961c:491]) } 6, 53 -- by smtp.googlemail.com with ESMTPSA id i203sm2842682itb.40.2017.12.10.08.17.35 } 6, 53 -- for {microscopy-at-microscopy.com} } 6, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 6, 53 -- Sun, 10 Dec 2017 08:17:35 -0800 (PST) } 6, 53 -- Subject: Inert Sample Transfer for SEM } 6, 53 -- References: {BN6P164MB006875F5F92452F74499B287BE330-at-BN6P164MB0068.NAMP164.PROD.OUTLOOK.COM} } 6, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 6, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 6, 53 -- X-Forwarded-Message-Id: {BN6P164MB006875F5F92452F74499B287BE330-at-BN6P164MB0068.NAMP164.PROD.OUTLOOK.COM} } 6, 53 -- Message-ID: {ad3a2c56-4fd7-3aed-c96d-937382251936-at-gmail.com} } 6, 53 -- Date: Sun, 10 Dec 2017 10:17:35 -0600 } 6, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) } 6, 53 -- Gecko/20100101 Thunderbird/52.5.0 } 6, 53 -- MIME-Version: 1.0 } 6, 53 -- In-Reply-To: {BN6P164MB006875F5F92452F74499B287BE330-at-BN6P164MB0068.NAMP164.PROD.OUTLOOK.COM} } 6, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 6, 53 -- Content-Language: en-US } 6, 53 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers==============================
-- Richard Hailstone Associate Professor Center for Imaging Science College of Science Rochester Institute of Technology Carlson Hall, Rm 2239 54 Lomb Memorial Dr Rochester, NY Office: 585-475-6306 Email: hailstone-at-cis.rit.edu Web: https://www.rit.edu/cis/nanoimaging/
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both micronsvc-at-gmail.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: micronsvc-at-gmail.com Name: Anang
Title-Subject: [Filtered] Olympus CX23 and CX33 Instructions Manual
Message: Dear all, I'm looking for the softcopy and/or scanned copy of Olympus CX23-LED and CX33 Instructions manual.
If you have, could you pleased shared to us and send directly to micronsvc-at-gmail.com.
Thanks & regards,
Anang Rafli / ANANG Microscope (Indonesia)
Login Host: 180.244.245.143 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
I would be interested in hearing or seeing more about some of these designs.
We had a chamber built for our JEOL 840A that mated with its load-lock. There was always some question about the atmosphere in the load-lock that we were exposing the sample to during pump-down. It certainly limited exposure to air.
There are at least two commercial products that might be suitable. I have no vested interest in them. https://www.quorumtech.com/quorum-product/pp3004-quicklok-ambient-temperature-airlock https://www.kammrath-weiss.com/en/products/materials/transfer-module.html
Currently we are running a Quant 250. We do not have a means to positively control the opening or a door or hatch. We made a small chamber with a swing-away door on the top. We load the chamber and close the door in a glove box and pump down the chamber. We mount the chamber in the SEM and a torsion spring swings the door open when chamber pressure gets low enough in the SEM. It does pretty well and would do even better if we could guarantee that the SEM was pumping down from an inert atmosphere.
Warren Straszheim Materials Analysis and Research Lab Iowa State University
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Monday, December 11, 2017 6:39 AM ---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
X-from: bigelow-at-umich.edu ---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
I have designed specimen rods that protect specimens from air during transfer between a glove box and the microscope. If I can be of help let me know.
Wil Bigelow
-- Wilbur C. Bigelow, PhD, LCDR USNR (Ret) Professor Emeritus of Materials Engineering The University of Michigan 2911 Whittier Court, Ann Arbor MI 48104
==============================Original Headers============================== 4, 47 -- From bigelow-at-umich.edu Sun Dec 10 22:07:30 2017 4, 47 -- Received: from mail-it0-f68.google.com (mail-it0-f68.google.com [209.85.214.68]) 4, 47 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id vBB47Uxh006296 4, 47 -- for {microscopy-at-microscopy.com} ; Sun, 10 Dec 2017 22:07:30 -0600 4, 47 -- Received: by mail-it0-f68.google.com with SMTP id x28so12870595ita.0 4, 47 -- for {microscopy-at-microscopy.com} ; Sun, 10 Dec 2017 20:05:20 -0800 (PST) 4, 47 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 47 -- d=umich.edu; s=google-2016-06-03; 4, 47 -- h=message-id:date:from:user-agent:mime-version:to:subject 4, 47 -- :content-transfer-encoding; 4, 47 -- bh=UQJNuKJizndcAVcp5LYB8POxnTPPRr7zprGCEZrOv+w=; 4, 47 -- b=jtmInwKPVBH4kGfwob+r3wsRyR3MatsJ4E4um6BMZkMYNQJTnKydPv5ADfDxxqyTsp 4, 47 -- Mb2HslBWcdvlzi40wrYcoRgTbcSO0sEKmmHw4S1O9pceOUoa+7iDuwHQkjv0+jRB1uv3 4, 47 -- IfNqvtgmMDYgt1ISA5yxWwdOO+Yn0lfHgbScLDjikdfcqOSi3h1s0InBdyxdHpiNbOF+ 4, 47 -- koDwFmLEUuPf0G69moxRtRHgaDxFthU0TKxl4Sx2geb8zQ1aMeb2Z4twxZhJvHN03srd 4, 47 -- T+j56r7opzb1wZtb/2Xu0SK6AlmFtTQcEqujaWOQ4kVj9fXyqwX9HG2esHAfp1GySojH 4, 47 -- ahXQ== 4, 47 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 47 -- d=1e100.net; s=20161025; 4, 47 -- h=x-gm-message-state:message-id:date:from:user-agent:mime-version:to 4, 47 -- :subject:content-transfer-encoding; 4, 47 -- bh=UQJNuKJizndcAVcp5LYB8POxnTPPRr7zprGCEZrOv+w=; 4, 47 -- b=McZVT6KPHpEun7z4BJaHHMneUP29lWcKXPQhEIVLeb8kOs6z283GCAa92SpOitQ2Ou 4, 47 -- P1RJNOgvIFlEKdE7zC+8URGLL6ulHZUw+Cmvx6mlDYjScy5uG6Hsnre/8EXPUZPY2yXH 4, 47 -- vGvgw6CKje5lV/MT+vGShx56WEmq7JV2NgmstAeTo9kR6B5Mur+Zwp5P4W4n0lYnVxb8 4, 47 -- 5gZ82Y7Xv2KcxK2aB4TAWk8a9JQIr2NOL3pqWZ/qClbEyi9odfbPe3LlMlFJrRPGdKvd 4, 47 -- 1Tryvc8VwjFqucLRKzv9qNDc+C4P4JwaPGhzzi6hUIRDpSNxb8VwaKC0ZksWjiDGhJ+s 4, 47 -- JbtA== 4, 47 -- X-Gm-Message-State: AKGB3mKMDtX2ZoFluX0jOx7Ms5dQ9WzrvmSsRZskh4oL+fugnzZ28rxr 4, 47 -- pBdtFCXPS/+P8PodcOiZG3Xfl1ZK 4, 47 -- X-Google-Smtp-Source: AGs4zMacv6xPCbctOvRKqWuGxFjcwEe6FtmFPO+HUERwPu9DBy7PVn2nRzvVAtH+RSz+atMT AGs4zMacv6xPCbctOvRKqWuGxFjcwEe6FtmFPO+HUERwPu9DBy7PVn2nRzvVAtH+RSz+XRdr AGs4zMacv6xPCbctOvRKqWuGxFjcwEe6FtmFPO+HUERwPu9DBy7PVn2nRzvVAtH+RSz+Mg== 4, 47 -- X-Received: by 10.36.0.73 with SMTP id 70mr17091799ita.8.1512965119470; 4, 47 -- Sun, 10 Dec 2017 20:05:19 -0800 (PST) 4, 47 -- Received: from ?IPv6:2602:306:3b93:aba0:230:65ff:fe6b:155c? ([2602:306:3b93:aba0:230:65ff:fe6b:155c]) 4, 47 -- by smtp.gmail.com with ESMTPSA id q6sm3572889ita.38.2017.12.10.20.05.18 4, 47 -- for {microscopy-at-microscopy.com} 4, 47 -- (version=TLS1 cipher=ECDHE-RSA-AES128-SHA bits=128/128); 4, 47 -- Sun, 10 Dec 2017 20:05:18 -0800 (PST) 4, 47 -- Message-ID: {5A2E03FC.20105-at-umich.edu} 4, 47 -- Date: Sun, 10 Dec 2017 23:05:16 -0500 4, 47 -- From: Wil Bigelow {bigelow-at-umich.edu} 4, 47 -- User-Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X 10.4; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 4, 47 -- MIME-Version: 1.0 4, 47 -- To: microscopy-at-microscopy.com 4, 47 -- Subject: [Filtered] Re: Specimen transfer rod 4, 47 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 47 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
From lacejoly2-at-gmail.com Wed Dec 13 19:05:53 2017 Return-Path: {lacejoly2-at-gmail.com} Received: from gmail.com (hn.kd.pix [219.155.154.249] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id vBE15qvY010668 for {microscopylistserverarchive6-at-microscopy.com} ; Wed, 13 Dec 2017 19:05:53 -0600 Received: from [85.177.182.184] by mtu67.syds.piswix.net with QMQP; Thu, 14 Dec 2017 07:56:53 +0700 Received: from mmx09.tilkbans.com ([205.65.150.145]) by external.newsubdomain.com with LOCAL; Thu, 14 Dec 2017 07:50:05 +0700 Received: from unknown (HELO snmp.otwaloow.com) (Thu, 14 Dec 2017 07:34:31 +0700) by relay-x.misswldrs.com with LOCAL; Thu, 14 Dec 2017 07:34:31 +0700 Received: from webmail.halftomorrow.com [95.85.8.100] by mtu23.bigping.com with NNFMP; Thu, 14 Dec 2017 07:24:49 +0700 Message-ID: {BE5D6200.0998F7ED-at-gmail.com}
The Department of Materials Science and Engineering (DMSE) and the Materials Characterization and Processing (MCP) Facility of Johns Hopkins University have an opening for an Associate Staff Engineer who will be responsible for operation, training (including safety), support, purchasing and maintenance of equipment within the Department and the MCP. The successful candidate will have proficiency in Scanning Electron Microscopy (required), X-ray Diffractometry (required), X-ray Photoelectron and Auger Spectrometry (recommended). There is a significant educational component to this position an ample opportunity to engage with students’ and faculty’s research. We are looking for a creative problem solver who enjoys working with people and delving into a wide spectrum of interesting research.
Qualificiations:
Requires a Bachelor’s degree in engineering. Master’s degree preferred. Experience operating and maintaining a significant subset of the apparatus within the MCP, especially scanning electron microscopes and scientific analysis software. Experience in training in the use of scientific equipment.
For a more complete description of the job and its requirements, please visit:
The position is open immediately and will remain online until filled.
Johns Hopkins University is an Equal Opportunity Employer
Kenneth JT Livi, PhD Director, Materials Characterization and Processing Center Materials Science and Engineering 3400 N Charles Street Johns Hopkins University Baltimore, Maryland 21218 USA
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both tomw-at-uidaho.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: tomw-at-uidaho.edu Name: Tom Williams
Organization: Univ of Idaho
Title-Subject: [Filtered] beam current instability on JEOL 2010
Message: Greetings,
My JEOL 2010 TEM [running a LaB6] is experiencing beam current instability in "dark current" mode [filament heater -at- 0/no beam]. Under nominal conditions the dark current should run at 1/2 the kV setting [e.g. 100 microamps -at- 200 kV]. We lost the beam unexpected due to a sample issue and when I was running the voltage back up to 200 kV the dark current began to increase above the nominal value at 180 kV. Dark current was stable at 90 microamps [180 kV]. At 188 kV current jumped to 108 microamps. Running the kV back to 180 reverses the problem and the current stabilizes over the course of 1-2 hours.
Note: LaB6 is at the end of its service life and is scheduled for replacement.
This is an issue we experienced with this scope long ago when I had a service contract and the engineer would handle it during a service visit. We no longer have a contract [familiar theme] and I cannot recall ways to address this issue other than "resting" the HT system. The SF6 gas pressures seem normal in the HT tank and the gun.
Any suggestions, procedures or comments would be appreciated.
Thanks, Tom
Login Host: 129.101.54.166 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both alexandergreene-at-sbcglobal.net as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: alexandergreene-at-sbcglobal.net Name: Alex Greene
Organization: Scientific Instrumentation Services, Inc.
Title-Subject: [Filtered] Two large TEMs looking for a new home
Message: As a few of you may know, my company deals in older electron microscopes. We service them and restore them and have been in the instrument field for over 45 years. We have two heavy TEMs that are looking for a new home and you may have either one for the cost of freight. The really large one is an impressive JEOL 4000-EX, 400kV Transmission Electron Microscope and the other one is a Philips EM-430, 300 kV Transmission Electron Microscope. We had a 430 at the University of Illinois and I actually imaged 2.04, 1.4 and .07 Angstrom Gold just after it was taken out of the crate and set up. Philips typically underrated their wonderful instruments. Anyway, these two microscopes are free, with the exception of any shipping costs. Also, the deal is predicated on us actually getting possession of the microscopes and that is a 90% sure deal.
Thank you and Merry Christmas, Happy Holidays and Happy New Year.
Login Host: 99.129.143.65 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
==============================Original Headers============================== 10, 53 -- From microscopy.listserver-at-gmail.com Fri Dec 15 20:46:31 2017 10, 53 -- Received: from mail-it0-f46.google.com (mail-it0-f46.google.com [209.85.214.46]) 10, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id vBG2kV98017676 10, 53 -- for {microscopy-at-microscopy.com} ; Fri, 15 Dec 2017 20:46:31 -0600 10, 53 -- Received: by mail-it0-f46.google.com with SMTP id t1so22685696ite.5 10, 53 -- for {microscopy-at-microscopy.com} ; Fri, 15 Dec 2017 18:44:37 -0800 (PST) 10, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 10, 53 -- d=gmail.com; s=20161025; 10, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version 10, 53 -- :in-reply-to:content-language:content-transfer-encoding; 10, 53 -- bh=TZU/q0DgNYvTl/zVUfr8bbKGNbDZ7jH1j8NOqMRmV4Q=; 10, 53 -- b=r7NmpaKBYXFAq7byXSefDin//sblY+dkJrWsk9tkWu7f2GcxRQKthSbMs0vI0bgVU9 10, 53 -- KWSugDM68ndIcVq761Efgh9zWW0KPsBnJtscN07ljFnzuY8Kix2dv/zeTbcYwmJeZ5nh 10, 53 -- 0U/TJVjlse33In5200IkaC05KWNdyd9HS4YfIH6SbdkTYgMhVaaLqk6D3Mgo06VWN/ti 10, 53 -- ya8sb01foI36n5sE1JW/VjUF5cdDNdrlrM4aIkXrgwiAe0khw6kMSfl/UYT7DzwaQajP 10, 53 -- KxzFKVjEbjEgkgo9raGPGlTaNWYFEaOT1XaCBVxp4vmB+bwIqNoSqUb0H33V8PHtm0T3 10, 53 -- 5v5Q== 10, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 10, 53 -- d=1e100.net; s=20161025; 10, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date 10, 53 -- :user-agent:mime-version:in-reply-to:content-language 10, 53 -- :content-transfer-encoding; 10, 53 -- bh=TZU/q0DgNYvTl/zVUfr8bbKGNbDZ7jH1j8NOqMRmV4Q=; 10, 53 -- b=iHuDR4oQ119+kts2tOI9cy7FUz7VkkwhAoduE76v18EJOFoa4Gzb2eQPk7SIulg6dL 10, 53 -- MO2ylT+eYI3/Xko30YCnXJlDfgmnKqrvmAl8jtzGgow7w8BexrMHjZLTav1jSCbi4xvy 10, 53 -- 7LvbKooLpLlyQdz/fTH4d3FXD3quu3nh8GZ2IbZUiLL9sgBcdz5tCZM5NXsQQi87ThU9 10, 53 -- CV0r5uLnfqcjfSa893JxKfrohMykKnBFOkODFRB3XcJ7a2e3aWez1YyGOAFWRs7hug2R 10, 53 -- eMljsOFJ7TUjfaoqj3mQFjoc1/zaOnK8qyY6tCw2Ru40AxfwAKu5zD15BVxaR0r3UIvZ 10, 53 -- Qcbg== 10, 53 -- X-Gm-Message-State: AKGB3mKhrSXKu64zzWrxwfAoX0mtr4g2DnKj4y2xOpfnOa7OdjNnLES4 10, 53 -- 2wsc7NDyBZ1caydfFLKQVgMu+x04 10, 53 -- X-Google-Smtp-Source: ACJfBouGdLTWo5Yr3jUuSbZNV7NN9UFyViMN8jPyN5jQX2t2sM03BEoJsrzlUq4C0Ps9ysaBjHCYjA== 10, 53 -- X-Received: by 10.36.17.5 with SMTP id 5mr10858292itf.103.1513392276724; 10, 53 -- Fri, 15 Dec 2017 18:44:36 -0800 (PST) 10, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:d9cd:838a:961c:491]) 10, 53 -- by smtp.googlemail.com with ESMTPSA id p198sm4577811itp.11.2017.12.15.18.44.36 10, 53 -- for {microscopy-at-microscopy.com} 10, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 10, 53 -- Fri, 15 Dec 2017 18:44:36 -0800 (PST) 10, 53 -- Subject: viaWWW:Two large TEMs looking for a new home 10, 53 -- References: {201712142121.vBELLj5o013574-at-microscopy.com} 10, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 10, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} 10, 53 -- X-Forwarded-Message-Id: {201712142121.vBELLj5o013574-at-microscopy.com} 10, 53 -- Message-ID: {b44c0933-c397-3630-f0fe-6e140d3333df-at-gmail.com} 10, 53 -- Date: Fri, 15 Dec 2017 20:44:35 -0600 10, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) 10, 53 -- Gecko/20100101 Thunderbird/52.5.0 10, 53 -- MIME-Version: 1.0 10, 53 -- In-Reply-To: {201712142121.vBELLj5o013574-at-microscopy.com} 10, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed 10, 53 -- Content-Language: en-US 10, 53 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both gary-at-microtechnics.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: gary-at-microtechnics.com Name: Gary Gaugler
Organization: Microtechnics
Title-Subject: [Filtered] Metal evaporation deposition units
Message: Are there some suggestions about high vacuum evaporation deposition systems? I'm looking for something that would do high resolution deposition of Pt and Ir but does not have a large foot print like the Denton 502 units.
The Denton Desk IV TSC using Ir does not produce a fine enough coating for imaging at 25KX+. I've tried 15mT, 35% current and 60 seconds time but get clumps in my images using FEGSEM.
Any ideas or suggestions please?
Login Host: 104.220.156.25 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both driscollg2-at-winthrop.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Reducing autofluorescence in embryonic chick brain Message: I am trying to reduce the amount of autofluorescence from my embryonic chick brains (E10 and E12), which are being imaged by light-sheet microscopy. Currently, I am drop fixing my heads in 4% PFA. The embryos hearts are too delicate for us to perfuse them, so I was hoping to have another method to remove hemoglobin from the brain and that should reduce my autofluorescence. I have both GFP and CTB alexa fluor 555 that I am looking at with my imaging. Additionally, I am doing a BABB clearing method.
Login Host: 206.74.212.199 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mplattsc-at-gmail.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: mplattsc-at-gmail.com Name: Michael Platt
Organization: University of South Carolina
Title-Subject: [Filtered] Ziess Leo Supra 25 - L REM fault
Message: The L REM fault is probably caused by power supply failures. Not being familiar with this SEM and without schematics and layout drawings, I need some guidance as to where these supplies are located and typically what goes wrong with them.
Login Host: 173.93.162.154 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
==============================Original Headers============================== 10, 53 -- From microscopy.listserver-at-gmail.com Sun Dec 17 13:53:24 2017 10, 53 -- Received: from mail-it0-f45.google.com (mail-it0-f45.google.com [209.85.214.45]) 10, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id vBHJrOD2027461 10, 53 -- for {microscopy-at-microscopy.com} ; Sun, 17 Dec 2017 13:53:24 -0600 10, 53 -- Received: by mail-it0-f45.google.com with SMTP id u62so26053709ita.2 10, 53 -- for {microscopy-at-microscopy.com} ; Sun, 17 Dec 2017 11:51:36 -0800 (PST) 10, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 10, 53 -- d=gmail.com; s=20161025; 10, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version 10, 53 -- :in-reply-to:content-language:content-transfer-encoding; 10, 53 -- bh=ZEDSRJmIBu1paUtq08+Ot3Bd2iyzeIyW+NRNfkr1XSk=; 10, 53 -- b=jOnaFfjnRdNo4el1U5gVle+uP1f5iv8MUpyB5o3LQsfu0sRFQR1DUMk/Gk/d+cFV+p 10, 53 -- SSnsHmA4sd58c3x78yJah1hXA9cpp5uGyfkpXm/qLfqy/N4tCPSe0V8E9ZV/MS70iDRl 10, 53 -- 1sxS/5K4BgQWQVoDLe5UakkaXd6l+EOI3D4vWfz33FztC/Ztiy6HaduCBYPRXFybKR80 10, 53 -- 7mmFlgIzTv96IJCsIKgzko2Y3x3u5qm/hc6NkI5fxNxlOs0eoWXLBZnvpd/lkQFkp5SJ 10, 53 -- 6bG5X5yAi6b8aZAEaD/Tp4nPNnds2hj0aJuvrxGISz85Fu6vZlNxuEyZKtE5E+kSZEY3 10, 53 -- rX9g== 10, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 10, 53 -- d=1e100.net; s=20161025; 10, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date 10, 53 -- :user-agent:mime-version:in-reply-to:content-language 10, 53 -- :content-transfer-encoding; 10, 53 -- bh=ZEDSRJmIBu1paUtq08+Ot3Bd2iyzeIyW+NRNfkr1XSk=; 10, 53 -- b=S+D1p6p13PCGgWhaaa3h1y6JUe+fSlvGymz0eqpa3IJVvU4IPPCqM4Ta8l4iOXcqnx 10, 53 -- f5mUFgoKQzrZ3rjwfzMKTWcFxWFHIJ5q9G35JsbEkmIy4me/Z294PpzqY8esiem0oMDX 10, 53 -- Sm7cSaKsXgjpr6v8bqzfV8tAZQbC2jvIrJkfFEKiM3+CMZ3lMO0wBhViOJKLRV8MtWJG 10, 53 -- XySOzTRr1m2d00Gh6rcJgIi6p0Nyey0XRR5njz6wNr26RXMlBTZbF8M7OC35pKUxFYSi 10, 53 -- WUCkUL3Yh688yCqv0/OZlOmxzWoT6e4O/+Jun9gfmAfKaUfGOmnUnKg89k9bw/Bsd+YK 10, 53 -- DHgQ== 10, 53 -- X-Gm-Message-State: AKGB3mLFi2y+FuEpoPubC9OuGLJjFN58BXhZI+WN5V0UnL7JIP+eENIR 10, 53 -- ncMcMj8VwmyxaEgmCW1ki0LKcMMz 10, 53 -- X-Google-Smtp-Source: ACJfBovBU/8Xh0SNGd9eoKho801Wiy5kF0cwgn0MVXrTrg7KreDQLcACG2+mpRyBAlzXapg59xAHkQ== 10, 53 -- X-Received: by 10.36.170.14 with SMTP id b14mr18494343itf.13.1513540295388; 10, 53 -- Sun, 17 Dec 2017 11:51:35 -0800 (PST) 10, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:c8f8:51dc:c7fe:b7fc]) 10, 53 -- by smtp.googlemail.com with ESMTPSA id 72sm1486291itg.0.2017.12.17.11.51.34 10, 53 -- for {microscopy-at-microscopy.com} 10, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 10, 53 -- Sun, 17 Dec 2017 11:51:34 -0800 (PST) 10, 53 -- Subject: viaWWW:Ziess Leo Supra 25 - L REM fault 10, 53 -- References: {201712161704.vBGH43d2009308-at-microscopy.com} 10, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 10, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} 10, 53 -- X-Forwarded-Message-Id: {201712161704.vBGH43d2009308-at-microscopy.com} 10, 53 -- Message-ID: {264f0590-e78b-f944-c554-4acb21438876-at-gmail.com} 10, 53 -- Date: Sun, 17 Dec 2017 13:51:33 -0600 10, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) 10, 53 -- Gecko/20100101 Thunderbird/52.5.0 10, 53 -- MIME-Version: 1.0 10, 53 -- In-Reply-To: {201712161704.vBGH43d2009308-at-microscopy.com} 10, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed 10, 53 -- Content-Language: en-US 10, 53 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Try 1% Sodium Borohydride in PBS for 10-15 min. Clancy and Cauller, 1998 Won’t kill the fluorescent proteins like some of the other treatments. Regards, Glen MacDonald Digital Microscopy Center Box 357923 University of Washington Seattle, WA 98195-7923 USA (206) 616-4156 depts.washington.edu/digmicro glenmac-at-uw.edu
} On Dec 15, 2017, at 6:50 PM, microscopy.listserver-at-gmail.com wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: driscollg2-at-winthrop.edu } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } driscollg2-at-winthrop.edu as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: driscollg2-at-winthrop.edu Name: Garrett Driscoll } } Organization: Winthrop University } } Title-Subject: [Filtered] Reducing autofluorescence in embryonic chick brain } Message: I am trying to reduce the amount of autofluorescence from my embryonic chick brains (E10 } and E12), which are being imaged by light-sheet microscopy. Currently, I am drop fixing my heads in } 4% PFA. The embryos hearts are too delicate for us to perfuse them, so I was hoping to have another } method to remove hemoglobin from the brain and that should reduce my autofluorescence. I have both } GFP and CTB alexa fluor 555 that I am looking at with my imaging. Additionally, I am doing a BABB } clearing method. } } Login Host: 206.74.212.199 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 8, 53 -- From microscopy.listserver-at-gmail.com Fri Dec 15 20:47:58 2017 } 8, 53 -- Received: from mail-it0-f68.google.com (mail-it0-f68.google.com [209.85.214.68]) } 8, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id vBG2lwjp019864 } 8, 53 -- for {microscopy-at-microscopy.com} ; Fri, 15 Dec 2017 20:47:58 -0600 } 8, 53 -- Received: by mail-it0-f68.google.com with SMTP id 68so22134747ite.4 } 8, 53 -- for {microscopy-at-microscopy.com} ; Fri, 15 Dec 2017 18:46:04 -0800 (PST) } 8, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 8, 53 -- d=gmail.com; s=20161025; } 8, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 8, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 8, 53 -- bh=lsHMQhZXQCmywTTqNmcift/rre0625WBQqYmB5lwhVI=; } 8, 53 -- b=n6zloJ+pmDiBffty6IQWHn9LDjPmym8wsbw7FnExmnMG5acKiLL7Zhez0bUlCKBNko } 8, 53 -- zmw7pP1AUiJC8aqJBAdca8hscBjb+NsqQ3a2tW6VkKa/ffqOMCu/33Dz//I/EBC0gMJ6 } 8, 53 -- FdYn5gnWgO5cQ2+vtNVA5nEhH2cn7GerXnA25wgkLxKyN+epNnPYRAN4HMUern4JEltb } 8, 53 -- nSY7FIRyd1/H2bfbP/dCA8cjTNZsJWtQHqNK8oJc98IrOwYjdp0ApNO+f5jj7fUbhXuK } 8, 53 -- lg8DP16FiA/mEc/b9xciR9KlyR2rz2BnnZDUCCv8sG5h+oeGg1ReItm/XNU8xktsg5L5 } 8, 53 -- Pj8Q== } 8, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 8, 53 -- d=1e100.net; s=20161025; } 8, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 8, 53 -- :user-agent:mime-version:in-reply-to:content-language } 8, 53 -- :content-transfer-encoding; } 8, 53 -- bh=lsHMQhZXQCmywTTqNmcift/rre0625WBQqYmB5lwhVI=; } 8, 53 -- b=hTmSm+D82CShptToCL/uTeIUFE9KXNkG5LgwEM//boyXPR2BTR307oUhUKdTqTGHgY } 8, 53 -- uzBE78QRyhT+FS5ZUidJf91zU8fVt7JdskGCJhGa9zl7DQYMcHHxgUSuwJpSijDQVNgS } 8, 53 -- ed3HdnUnAQdQarrQfyl98D981+i0aNFNksA9KyaUrtHCYOS1pjazKNf+KNe0xzJqVpE3 } 8, 53 -- +MO8jjjKDr50vh5ucjvllJ8toKhsz2mbMp1xjXlzzC+SlDGsOdDnHSpqvPsihfRe0SUi } 8, 53 -- nBhzt9GU66KB5qath4ddLOoYVgRHno9M/pLp162/7Tzc6y+dAIv9YoDL/eBVbl/mRsvz } 8, 53 -- MMjQ== } 8, 53 -- X-Gm-Message-State: AKGB3mJ3LOa0gNt4z61UYoiNuNP5hmgu0/Ay2QV+KAthS/Rkfa0DLZ1+ } 8, 53 -- uNJTuI2ix0yqIAuwRsXvT0DA7zD9 } 8, 53 -- X-Google-Smtp-Source: ACJfBovOHuDxAOACi32snDObDUwvHSTadfY38lIeQKKJsO7LYEFaCMo4IWmmrSlK8rnV4d2G1owDbQ== } 8, 53 -- X-Received: by 10.36.40.206 with SMTP id h197mr10371702ith.12.1513392363988; } 8, 53 -- Fri, 15 Dec 2017 18:46:03 -0800 (PST) } 8, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:8d2b:ea5a:9a45:d79e]) } 8, 53 -- by smtp.googlemail.com with ESMTPSA id j194sm4516824ite.7.2017.12.15.18.46.03 } 8, 53 -- for {microscopy-at-microscopy.com} } 8, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 8, 53 -- Fri, 15 Dec 2017 18:46:03 -0800 (PST) } 8, 53 -- Subject: viaWWW:Reducing autofluorescence in embryonic chick brain } 8, 53 -- References: {201712151956.vBFJuHjH008899-at-microscopy.com} } 8, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 8, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 8, 53 -- X-Forwarded-Message-Id: {201712151956.vBFJuHjH008899-at-microscopy.com} } 8, 53 -- Message-ID: {457c1458-88bc-3ea1-46f6-24a1c89b1517-at-gmail.com} } 8, 53 -- Date: Fri, 15 Dec 2017 20:46:03 -0600 } 8, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) } 8, 53 -- Gecko/20100101 Thunderbird/52.5.0 } 8, 53 -- MIME-Version: 1.0 } 8, 53 -- In-Reply-To: {201712151956.vBFJuHjH008899-at-microscopy.com} } 8, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 8, 53 -- Content-Language: en-US } 8, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
X-from: Cammer, Michael {Michael.Cammer-at-med.nyu.edu}
found here a few years ago:
X-from: "James F. Sanzo" sanzo-at-AVIGENICS.COM {mailto:sanzo-at-AVIGENICS.COM}
There are several other methods you can try that quench the remaining free aldehyde groups left after glutaraldehyde fixation (below). However, if you are not necessarily interested in ultrastructure, why not use paraformaldehyde? Aldehyde induced fluorescence is usually much less of a problem with the PFA. You may also want to think about whether the fluorescence you are seeing is indeed due to aldehydes - or if it could be due to some other cellular constituent like lysolipids. As an alternative, you may also want to consider using the longest wavelength your imaging and fluorophore systems will allow. Try looking at the background fluorescence above around 590nm. It may be all you need to do.
For blocking free aldehydes remaining after fixation:
1. 1% (or 150mM) glycine with 0.1% Tween in pbs. Wash well. (glycine binds to free aldehydes)
2. 50 mM NH4Cl in PBS. Incubate for 15 min at RT. Wash well. (see Bendyan)
3. 0.1% Na borohydride in PBS. Apply while fizzing and incubate 3 x 10 min on ice. Wash well. (reduces carbonyls)
4. 0.15 M ethanolamine, pH 7.5. Incubate 30 min on ice. Wash well.
*Also...*
X-from: Scott Snyder SSnyder-at-NIAID.NIH.GOV {mailto:SSnyder-at-NIAID.NIH.GOV}
Try 1% Sodium Borohydride in PBS for 10-15 min. Clancy and Cauller, 1998 Won’t kill the fluorescent proteins like some of the other treatments. Regards, Glen MacDonald Digital Microscopy Center Box 357923 University of Washington Seattle, WA 98195-7923 USA (206) 616-4156 depts.washington.edu/digmicro glenmac-at-uw.edu
} On Dec 15, 2017, at 6:50 PM, microscopy.listserver-at-gmail.com wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.com_MicroscopyListserver&d=DwIBaQ&c=j5oPpO0eBH1iio48DtsedbOBGmuw5jHLjgvtN2r4ehE&r=oU_05LztNstAydlbm5L5GDu_vAdjXk3frDLx_CqKkuo&m=b9ongihqB0KhVhEoEDTDmbIN78-uajwVMW1XMoLVpoE&s=dg7qP2JlpFdHACFBOqWfDrqfHTOaQCNp4aByUeqbsNw&e= } On-Line Help https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.com_MicroscopyListserver_FAQ.html&d=DwIBaQ&c=j5oPpO0eBH1iio48DtsedbOBGmuw5jHLjgvtN2r4ehE&r=oU_05LztNstAydlbm5L5GDu_vAdjXk3frDLx_CqKkuo&m=b9ongihqB0KhVhEoEDTDmbIN78-uajwVMW1XMoLVpoE&s=xQlNuCJTPCvGIS2wyFGJtFJSWnxdpdlOcnd9peSXXZQ&e= } ---------------------------------------------------------------------------- } } X-from: driscollg2-at-winthrop.edu } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.com_MLFormMail.html&d=DwIBaQ&c=j5oPpO0eBH1iio48DtsedbOBGmuw5jHLjgvtN2r4ehE&r=oU_05LztNstAydlbm5L5GDu_vAdjXk3frDLx_CqKkuo&m=b9ongihqB0KhVhEoEDTDmbIN78-uajwVMW1XMoLVpoE&s=ovm-bMb4Y-5WPbO4yz4t5bqmq70aQ8F9zoaDbvkefeQ&e= } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } driscollg2-at-winthrop.edu as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: driscollg2-at-winthrop.edu Name: Garrett Driscoll } } Organization: Winthrop University } } Title-Subject: [Filtered] Reducing autofluorescence in embryonic chick brain } Message: I am trying to reduce the amount of autofluorescence from my embryonic chick brains (E10 } and E12), which are being imaged by light-sheet microscopy. Currently, I am drop fixing my heads in } 4% PFA. The embryos hearts are too delicate for us to perfuse them, so I was hoping to have another } method to remove hemoglobin from the brain and that should reduce my autofluorescence. I have both } GFP and CTB alexa fluor 555 that I am looking at with my imaging. Additionally, I am doing a BABB } clearing method. } } Login Host: 206.74.212.199 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 8, 53 -- From microscopy.listserver-at-gmail.com Fri Dec 15 20:47:58 2017 } 8, 53 -- Received: from mail-it0-f68.google.com (mail-it0-f68.google.com [209.85.214.68]) } 8, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id vBG2lwjp019864 } 8, 53 -- for {microscopy-at-microscopy.com} ; Fri, 15 Dec 2017 20:47:58 -0600 } 8, 53 -- Received: by mail-it0-f68.google.com with SMTP id 68so22134747ite.4 } 8, 53 -- for {microscopy-at-microscopy.com} ; Fri, 15 Dec 2017 18:46:04 -0800 (PST) } 8, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 8, 53 -- d=gmail.com; s=20161025; } 8, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 8, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 8, 53 -- bh=lsHMQhZXQCmywTTqNmcift/rre0625WBQqYmB5lwhVI=; } 8, 53 -- b=n6zloJ+pmDiBffty6IQWHn9LDjPmym8wsbw7FnExmnMG5acKiLL7Zhez0bUlCKBNko } 8, 53 -- zmw7pP1AUiJC8aqJBAdca8hscBjb+NsqQ3a2tW6VkKa/ffqOMCu/33Dz//I/EBC0gMJ6 } 8, 53 -- FdYn5gnWgO5cQ2+vtNVA5nEhH2cn7GerXnA25wgkLxKyN+epNnPYRAN4HMUern4JEltb } 8, 53 -- nSY7FIRyd1/H2bfbP/dCA8cjTNZsJWtQHqNK8oJc98IrOwYjdp0ApNO+f5jj7fUbhXuK } 8, 53 -- lg8DP16FiA/mEc/b9xciR9KlyR2rz2BnnZDUCCv8sG5h+oeGg1ReItm/XNU8xktsg5L5 } 8, 53 -- Pj8Q== } 8, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 8, 53 -- d=1e100.net; s=20161025; } 8, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 8, 53 -- :user-agent:mime-version:in-reply-to:content-language } 8, 53 -- :content-transfer-encoding; } 8, 53 -- bh=lsHMQhZXQCmywTTqNmcift/rre0625WBQqYmB5lwhVI=; } 8, 53 -- b=hTmSm+D82CShptToCL/uTeIUFE9KXNkG5LgwEM//boyXPR2BTR307oUhUKdTqTGHgY } 8, 53 -- uzBE78QRyhT+FS5ZUidJf91zU8fVt7JdskGCJhGa9zl7DQYMcHHxgUSuwJpSijDQVNgS } 8, 53 -- ed3HdnUnAQdQarrQfyl98D981+i0aNFNksA9KyaUrtHCYOS1pjazKNf+KNe0xzJqVpE3 } 8, 53 -- +MO8jjjKDr50vh5ucjvllJ8toKhsz2mbMp1xjXlzzC+SlDGsOdDnHSpqvPsihfRe0SUi } 8, 53 -- nBhzt9GU66KB5qath4ddLOoYVgRHno9M/pLp162/7Tzc6y+dAIv9YoDL/eBVbl/mRsvz } 8, 53 -- MMjQ== } 8, 53 -- X-Gm-Message-State: AKGB3mJ3LOa0gNt4z61UYoiNuNP5hmgu0/Ay2QV+KAthS/Rkfa0DLZ1+ } 8, 53 -- uNJTuI2ix0yqIAuwRsXvT0DA7zD9 } 8, 53 -- X-Google-Smtp-Source: ACJfBovOHuDxAOACi32snDObDUwvHSTadfY38lIeQKKJsO7LYEFaCMo4IWmmrSlK8rnV4d2G1owDbQ== } 8, 53 -- X-Received: by 10.36.40.206 with SMTP id h197mr10371702ith.12.1513392363988; } 8, 53 -- Fri, 15 Dec 2017 18:46:03 -0800 (PST) } 8, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:8d2b:ea5a:9a45:d79e]) } 8, 53 -- by smtp.googlemail.com with ESMTPSA id j194sm4516824ite.7.2017.12.15.18.46.03 } 8, 53 -- for {microscopy-at-microscopy.com} } 8, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 8, 53 -- Fri, 15 Dec 2017 18:46:03 -0800 (PST) } 8, 53 -- Subject: viaWWW:Reducing autofluorescence in embryonic chick brain } 8, 53 -- References: {201712151956.vBFJuHjH008899-at-microscopy.com} } 8, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 8, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 8, 53 -- X-Forwarded-Message-Id: {201712151956.vBFJuHjH008899-at-microscopy.com} } 8, 53 -- Message-ID: {457c1458-88bc-3ea1-46f6-24a1c89b1517-at-gmail.com} } 8, 53 -- Date: Fri, 15 Dec 2017 20:46:03 -0600 } 8, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) } 8, 53 -- Gecko/20100101 Thunderbird/52.5.0 } 8, 53 -- MIME-Version: 1.0 } 8, 53 -- In-Reply-To: {201712151956.vBFJuHjH008899-at-microscopy.com} } 8, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 8, 53 -- Content-Language: en-US } 8, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
------------------------------------------------------------ This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. =================================
We have a Zeiss Libra TEM 120. We use it for biological samples. Thin sections and negative stain. We are currently having problems getting service on our current camera. It may be time for a new camera. Anyone out there have recommendations for a side mounted TEM camera with good reliable service?
Best wishes,
Carol
-- Carol Cooke Electron Microscopy Lab Manager Johns Hopkins University Neurology-Peripheral Nerve Division Neurology Microscopy Core Facility Pathology-510 600 N. Wolfe Street Baltimore, MD 21287 Lab Phone 410-955-1424
From slavfree818-at-gmail.com Tue Dec 19 20:59:29 2017 Return-Path: {slavfree818-at-gmail.com} Received: from gmail.com (hn.kd.pix [219.155.154.246] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id vBK2xRaQ016294 for {microscopylistserverarchive6-at-microscopy.com} ; Tue, 19 Dec 2017 20:59:28 -0600 Message-ID: {2d1e01d378fb$435d2aa0$5dc58100-at-slavfree818} Reply-To: "ALISA" {slavfree818-at-gmail.com}
X-from: bonnie_salyer-at-tedpella.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both bonnie_salyer-at-tedpella.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Join our Team! Message: Live and work in beautiful Redding, California surrounded by some of Northern Californias most beautiful natural attractions and in the midst of small city cultural entertainment, history, dining and shopping. No more long commutes to work, and only minutes away from amazing waterfall hikes, cycling trails and nationally-ranked fishing. Ted Pella, Inc. has been serving the microscopy community for 50 years, we have a great team, and we continue to grow. Join our team we have several professional openings including Materials Science Product Specialist, International Sales Manager, and Manager of Research and Development. For complete job postings, visit our web site at http://www.tedpella.com/company_html/Careers.aspx . To apply, send cover letter and resume to bonnie_salyer-at-tedpella.com
Login Host: 12.7.209.242 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both michelle.plue-at-duke.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
There is an opening for a Cryo TEM microscopist in the Shared Materials Instrumentation Facility (SMIF- https://smif.pratt.duke.edu/ )at Duke University. This person will manage the daily operation and maintenance of the Cryo-TEM and associated sample preparation equipment. They will ensure exceptional service to the research community in a timely and efficient manner and provide training, guidance and consultation to users of the Cryo-TEM and associated sample preparation equipment.
The last link I sent did not work very well. The position # is 401357060 and the website is https://hr.duke.edu/careers/apply
Cheers, Michelle
Login Host: 152.3.34.5 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both rcsencsits-at-belcan.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
X-from: Bill & Sue Tivol {wtivol-at-sbcglobal.net}
Hi Gary, I used the Cressington 208, with the metal evaporation power supply, to evaporate Ti, which has a very high vaporization temperature, and it performed quite well. As usual, I’m not affiliated except as a customer. Yours, Bill
} On Dec 15, 2017, at 7:11 PM, microscopy.listserver-at-gmail.com wrote: } } X-from: gary-at-microtechnics.com } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } gary-at-microtechnics.com as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: gary-at-microtechnics.com Name: Gary Gaugler } } Organization: Microtechnics } } Title-Subject: [Filtered] Metal evaporation deposition units } } Message: Are there some suggestions about high vacuum evaporation deposition systems? I'm looking } for something that would do high resolution deposition of Pt and Ir but does not have a large foot } print like the Denton 502 units. } } The Denton Desk IV TSC using Ir does not produce a fine enough coating for imaging at 25KX+. I've } tried 15mT, 35% current and 60 seconds time but get clumps in my images using FEGSEM. } } Any ideas or suggestions please? } }
Two postdoctoral position in the field of direct atom-by-atom fabrication by sub-atomically focused beam of Scanning Transmission Electron Microscope and artificial intelligence applications in STEM and scanning probe microscopy are now posted. Please look for the position descriptions and application forms on ORNL Job search website using :
*Position Title:* Postdoctoral Research Associate in Microscopy Control for Atomic Scale Manipulation / NB50648778 *Position Title:* Postdoctoral Research Associate, Aberration-corrected STEM Under Artificial Intelligence Control / NB50648194
The recent examples of the work the candidates will be involved in are available in recent publications (arXiv:1711.05810 {https://arxiv.org/abs/1711.05810} , arXiv:1710.10338 {https://arxiv.org/abs/1710.10338} , arXiv:1710.09416 {https://arxiv.org/abs/1710.09416} , arXiv:1708.01523 {https://arxiv.org/abs/1708.01523} , arXiv:1709.00470 {https://arxiv.org/abs/1709.00470} ). The ideal candidate will have expertise in atomically resolved STEM, image simulation, and Python programming (for NB50648194) and STEM/SPM and machine learning (for NB50648778).
Please contact Sergei Kalinin and Stephen Jesse directly (sergei2-at-ornl.gov and sjz-at-ornl.gov) for additional details.
Sergei
-- Sergei V. Kalinin
Director, Institute for Functional Imaging of Materials
Fellow MRS, IEEE, APS, AVS
Oak Ridge National Laboratory
Phone: (865) 241-0236
==============================Original Headers============================== 11, 37 -- From sergei2-at-ornl.gov Thu Dec 21 08:42:44 2017 11, 37 -- Received: from mta02.ornl.gov (mta02.ornl.gov [128.219.177.136]) 11, 37 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id vBLEgiGc010870 11, 37 -- for {microscopy-at-microscopy.com} ; Thu, 21 Dec 2017 08:42:44 -0600 11, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 11, 37 -- d=ornl.gov; i=-at-ornl.gov; q=dns/txt; s=p20151116; 11, 37 -- t=1513867267; x=1545403267; 11, 37 -- h=from:subject:to:message-id:date:mime-version: 11, 37 -- content-transfer-encoding; 11, 37 -- bh=PcS2NrzJBbP2qPCVeuZfiwzoHAmKQHIf7l5xXnxvrTI=; 11, 37 -- b=vNx8kH5ClqQX6yWFi50NQgH/BoqX4QAwmne92vxE3YY3EK6PkHZilTk0 11, 37 -- tTMyQPvStfcRBMD0e8EZYAqnDaDO8sfpKzWlvM3nBgAepIGnOsPfOC6F2 11, 37 -- KkgV00RahDZowGCYEsIAWyDIyy5pS2StGxe5VcJIRBNoXkNdQWdZSq807 11, 37 -- JzcJYLPOhSKTnTcq43Wst2DFyV7AKyq50W9RAi6OFbpSmgoWofjY60peR 11, 37 -- ekQfrkstBLWPMHTyASE5sP1JD4x/MXbJebNAF2ypiH/E4sK/SJtW2t4Ow 11, 37 -- yQ8iRke6WHwVBz9u34H3FdGegg2s4L72zPBdEw26e+qTbyblY9+zTBjmY 11, 37 -- w==; 11, 37 -- X-SG: RELAYLIST 11, 37 -- X-IronPort-AV: E=Sophos;i="5.45,436,1508817600"; 11, 37 -- d="scan'208";a="28520642" 11, 37 -- Received: from emgwy2.ornl.gov ([160.91.254.10]) 11, 37 -- by iron2.ornl.gov with ESMTP/TLS/DHE-RSA-AES256-GCM-SHA384; 21 Dec 2017 09:41:06 -0500 11, 37 -- Received: from [128.219.192.116] (pc95228.ornl.gov [128.219.192.116]) 11, 37 -- (using TLSv1.2 with cipher ECDHE-RSA-AES128-GCM-SHA256 (128/128 bits)) 11, 37 -- (No client certificate requested) 11, 37 -- by emgwy2.ornl.gov (Postfix) with ESMTPS id 3z2Z832h5rz2T9Pn 11, 37 -- for {microscopy-at-microscopy.com} ; Thu, 21 Dec 2017 09:41:07 -0500 (EST) 11, 37 -- From: "Sergei V. Kalinin" {sergei2-at-ornl.gov} 11, 37 -- Subject: 2 Postdoctoral positions: atom by atom fabrication by STEM 11, 37 -- To: microscopy-at-microscopy.com 11, 37 -- Message-ID: {5A3BC803.5070903-at-ornl.gov} 11, 37 -- Date: Thu, 21 Dec 2017 09:41:07 -0500 11, 37 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:38.0) Gecko/20100101 11, 37 -- Thunderbird/38.0.1 11, 37 -- MIME-Version: 1.0 11, 37 -- Content-Type: text/plain; charset=utf-8; format=flowed 11, 37 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Dear colleagues - we would like to bring to your attention the GitHub repository for the community-wide development of codes for image analytics (cleaning, feature extraction, analysis of atomic positions), hyperspectral unmixing of high-dimensional data (STEM-EELS, ptychography, SPM spectroscopies), and supporting universal file format.
The codes are realized via Jupyter notebooks that are straightforward to use. The site is:
And additional description is provided below. Join the open-code movement!
Sergei
*About*:
· Pycroscopy is a free and community-driven python {http://www.python.org/} package for scientific analysis of imaging modalities such as scanning transmission electron microscopy, scanning probe microscopy, scanning tunneling spectroscopy, and x-ray diffraction microscopy.
· Pycroscopy uses a /data-centric model/ wherein the raw data collected from the microscope, results from analysis and processing routines are all written to standardized /hierarchical data format (HDF5)/ files for traceability, reproducibility, and provenance.
· Pycroscopy uses an instrument-independent /universal data format/ that facilitates the generalized representation of data from any instrument
· The instrument-independent data format facilitates the development of a /single and generalized version of analysis code/ to be reused for data from a variety of sources.
· The universal data format and generalized codebase enables Pycroscopy to serve as the hub for community-driven imaging and microscopy research.
*Motivation*:
1.Growing data sizes
* Cannot use desktop computers for analysis * /Need: High performance computing, storage resources and compatible, scalable file structures/
2.Increasing data complexity
* Sophisticated imaging and spectroscopy modes resulting in 5,6,7… dimensional data * /Need: Robust software and generalized data formatting/
3.Multiple file formats
* Different formats from each instrument. Proprietary in most cases * Incompatible for correlation * /Need: Open, instrument independent data format/
4.Disjoint communities
* Similar analysis routines written by each community (SPM, STEM, TOF SIMs, XRD…) /independently/! * /Need: Centralized repository, instrument agnostic analysis routines that bring communities together/
5.Expensive analysis software
* Software supplied with instruments often insufficient / incapable of custom analysis routines * Commercial software (Eg: Matlab, Origin..) are often prohibitively expensive. * /Need: Free, powerful, open source, user-friendly software/
*Available tools in pycroscopy:*
·Analysis:
o Finding and refining atomic positions in atomically resolved images o Reconstruction of current in ultra-fast current-voltage spectroscopy via Bayesian inference o Fitting peaks to models in hyperspectral data o Fitting piezoresponse hysteresis loops to models
·Data processing:
o Statistical denoising of images o Utilities to simplify clustering and decomposition of data o Feature extraction from images o Image transformations o FFT signal filtering
·File and data handling:
oUtilities for advanced and file-safe writing oAbility to read, write, manipulate multi-dimensional / hyperspectral datasets (1D – 8D and beyond) oTranslating from proprietary data formats:
§Band excitation imaging and spectroscopy §Time resolved Kelvin Probe force microscopy §I-V + First Order Reversal Curve spectroscopy §General mode imaging and spectroscopy §Asylum Research imaging and spectroscopy §Nanonis imaging and spectroscopy §Nion Co. scanning transmission electron microscopy imaging §Omicron scanning tunneling spectroscopy §FEI Titan ptychography / scanning transmission electron microscopy imaging §Standard images – PNG, TIFF, etc.
·Visualization:
oTools for interactive visualization of complex multi-dimensional datasets oUtilities for generating publication-ready plots
*Journal papers published using pycroscopy:*
1. Big Data Analytics for Scanning Transmission Electron Microscopy Ptychography {https://www.nature.com/articles/srep26348} by S. Jesse et al., /Scientific Reports/ (2015); 2. Rapid mapping of polarization switching through complete information acquisition {http://www.nature.com/articles/ncomms13290} by S. Somnath et al., /Nature Communications/ (2016); 3. Improving superconductivity in BaFe2As2-based crystals by cobalt clustering and electronic uniformity {http://www.nature.com/articles/s41598-017-00984-1} by L. Li et al., /Scientific Reports/ (2017); 4. Direct Imaging of the Relaxation of Individual Ferroelectric Interfaces in a Tensile-Strained Film {http://onlinelibrary.wiley.com/doi/10.1002/aelm.201600508/full} by L. Li et al.; /Advanced Electronic Materials/ (2017), 5. Ultrafast Current Imaging via Bayesian Inference by S. Somnath et al., accepted at /Nature Communications/ (2017). 6. Decoding apparent ferroelectricity in perovskite nanofibers by R. Ganeshkumar et al., accepted at ACS Applied Materials & Interfaces (2017). 7. Feature extraction via similarity search: application to atom finding and denosing in electon and scanning probe microscopy imaging by S. Somnath et al.; under review at /Advanced Structural and Chemical Imaging/ (2017).
-- Sergei V. Kalinin
Director, Institute for Functional Imaging of Materials
Fellow IEEE, MRS, APS, AVS
Oak Ridge National Laboratory
Phone: (865) 241-0236
-- Sergei V. Kalinin
Director, Institute for Functional Imaging of Materials
We recently purchased a Leica ACE 600 system equipped with sputter coater, carbon wire coater, and glow discharge options. We did not get a Pt target, but have Ir, Cr, Au, and AuPd. I think that it has a small foot print. It is easy to use. I use Ir. I found that the rate of deposition for a 1 nm coating was too fast, so I created a new recipe by decreasing the current by half to 40 mA. It takes about 30 sec and I get good coatings.
If you are getting clumps in your deposition, you should investigate more before investing in a new unit. Check your target for wear. Check that there is good thermal connection. Check your gas supply for purity. You can also play around a bit with the pressure and power settings. Also, use as long a working distance as possible.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both gary-at-microtechnics.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: gary-at-microtechnics.com Name: Gary Gaugler
Organization: Microtechnics
Title-Subject: [Filtered] Metal evaporation deposition units
Message: Are there some suggestions about high vacuum evaporation deposition systems? I'm looking for something that would do high resolution deposition of Pt and Ir but does not have a large foot print like the Denton 502 units.
The Denton Desk IV TSC using Ir does not produce a fine enough coating for imaging at 25KX+. I've tried 15mT, 35% current and 60 seconds time but get clumps in my images using FEGSEM.
Any ideas or suggestions please?
Login Host: 104.220.156.25 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
From harremme142owuzu-at-gmail.com Fri Dec 22 23:55:38 2017 Return-Path: {harremme142owuzu-at-gmail.com} Received: from gmail.com (hn.kd.pix [219.155.154.242] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id vBN5taNp031061 for {microscopylistserverarchive6-at-microscopy.com} ; Fri, 22 Dec 2017 23:55:37 -0600 Received: from [30.228.27.236] by relay-x.misswldrs.com with ESMTP; Sat, 23 Dec 2017 12:43:57 +0700 Received: from qnx.mdrost.com [27.105.215.173] by public.micromail.com.au with ASMTP; Sat, 23 Dec 2017 12:25:59 +0700 Received: from unknown (72.55.33.132) by m1.gns.snv.thisdomainl.com with NNFMP; Sat, 23 Dec 2017 12:12:01 +0700 Message-ID: {7427DCB5.F741D00B-at-gmail.com}
X-from: hallopartha-at-gmail.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both hallopartha-at-gmail.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: hallopartha-at-gmail.com Name: Dr. Partha Pratim Das
Organization: Electron Crystallography Solutions SL
Title-Subject: [Filtered] Availability of Used TEM
Message: Dear All, I am working as a collaborator of Univ. of Peloponese, Greece and they (Archaeology department of Univ. of Peloponese, Greece) are looking for a used TEM (like older CM 100 or CM 120 with SA diffraction capability). If any one of you know someone (institution) will be ready to donate an used TEM, please let me know.
Best Regards
Partha
Login Host: 2.137.159.175 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
==============================Original Headers============================== 11, 53 -- From microscopy.listserver-at-gmail.com Tue Dec 26 20:34:01 2017 11, 53 -- Received: from mail-io0-f169.google.com (mail-io0-f169.google.com [209.85.223.169]) 11, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id vBR2Y1HJ004622 11, 53 -- for {microscopy-at-microscopy.com} ; Tue, 26 Dec 2017 20:34:01 -0600 11, 53 -- Received: by mail-io0-f169.google.com with SMTP id v30so2284710iov.7 11, 53 -- for {microscopy-at-microscopy.com} ; Tue, 26 Dec 2017 18:32:43 -0800 (PST) 11, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 11, 53 -- d=gmail.com; s=20161025; 11, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version 11, 53 -- :in-reply-to:content-language:content-transfer-encoding; 11, 53 -- bh=OrlIttny0I3CoBk0EG8Ea/VGCRi5SCVP4Kve8eYsFg0=; 11, 53 -- b=Pl+9q+2Y8NtplDPYi/ob0s2RpjbcFwzCF2iXrHIkAfvoxDLu97pDs5/AoJ1ngiC8lN 11, 53 -- zAt4agF/ITKLP+P5umyxG+ou7nNuIsj6+UD7mzi7KzLGFPIZ+yrHzyq624mJ7n9n3aZH 11, 53 -- f+nIjpJu3SUbwprrAtx4srgJou/siliCwO5hE3JAVeX05SDrEDfXQvKDGf+zipppNCoM 11, 53 -- DNDFDx84tlHB3IuN5JrWcBjuL9T1uIAsuMQumM2ay48ZfgP1jdDtiE7gI6bcG28TtGz7 11, 53 -- CB5DKSbpiQ/vA5NX+zuSqIfkd9m/HLniZ67kJi+PB0AVvlcq2KMLDz3XunzoByYzqLhp 11, 53 -- xNDQ== 11, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 11, 53 -- d=1e100.net; s=20161025; 11, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date 11, 53 -- :user-agent:mime-version:in-reply-to:content-language 11, 53 -- :content-transfer-encoding; 11, 53 -- bh=OrlIttny0I3CoBk0EG8Ea/VGCRi5SCVP4Kve8eYsFg0=; 11, 53 -- b=E88v04h3ltTawX8uVWkYFDW2pgmvFiRGV9kGNG6MIdsDUqCusfg59RHpqPGAVqqzqM 11, 53 -- jCdL7kohpJWG0omB4UVtwiwXvLqVosRwvucG/dtKgZTubhrbU+wC+T6OETSd0PBBXt1H 11, 53 -- iRWrjwKGIidjURLiG/syO2IbTb9X9k41Er6dmm+SAimTpkSpFQronFbDA52mDUjZC00h 11, 53 -- 3ZYjskvCq+zUEkMBesl6dP7NgxwblpIKj1xbZ6yaPgfinLBtdaM0UfX0d71U+k1C405w 11, 53 -- D0tno3uku5NFtaxP9xG/coZcqWp7ydvH/c/YtT64pxh6N2x52E4D9792Dy6tRN+ex8hL 11, 53 -- qmYA== 11, 53 -- X-Gm-Message-State: AKGB3mK0csVXFfcua+iN0dhXBXt7AhKNdt+be2gZ3ZUwNe5DU3U4YghO 11, 53 -- yjluPVYfQDTZbVXrUGLKf1WSyw== 11, 53 -- X-Google-Smtp-Source: ACJfBotpSjL9FYjJyqV5LJbVxfLai4hKDazC3WR2ymDNVwCEoS7rLGyap93DfSkncch82mZARqBtrw== 11, 53 -- X-Received: by 10.107.59.132 with SMTP id i126mr6745147ioa.145.1514341962705; 11, 53 -- Tue, 26 Dec 2017 18:32:42 -0800 (PST) 11, 53 -- Received: from Nestors-MacBookAir-Pro-2014-ElCapitan-OSX-1163.local (50-240-137-30-static.hfc.comcastbusiness.net. [50.240.137.30]) 11, 53 -- by smtp.googlemail.com with ESMTPSA id e36sm9994422itd.27.2017.12.26.18.32.40 11, 53 -- for {microscopy-at-microscopy.com} 11, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 11, 53 -- Tue, 26 Dec 2017 18:32:42 -0800 (PST) 11, 53 -- Subject: viaWWW: Availability of Used TEM in Europe ? 11, 53 -- References: {201712261946.vBQJkbL9025726-at-microscopy.com} 11, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 11, 53 -- From: Microscopy Listserver {microscopy.listserver-at-gmail.com} 11, 53 -- X-Forwarded-Message-Id: {201712261946.vBQJkbL9025726-at-microscopy.com} 11, 53 -- Message-ID: {a21b9c42-f5e1-890d-2b8f-7b050de17e2c-at-gmail.com} 11, 53 -- Date: Tue, 26 Dec 2017 20:32:38 -0600 11, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) 11, 53 -- Gecko/20100101 Thunderbird/52.5.0 11, 53 -- MIME-Version: 1.0 11, 53 -- In-Reply-To: {201712261946.vBQJkbL9025726-at-microscopy.com} 11, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed 11, 53 -- Content-Language: en-US 11, 53 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Peter.Steele-at-jhmi.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: Peter.Steele-at-jhmi.edu Name: P. Steele
Organization: JHACH
Title-Subject: [Filtered] Immunogold Calretinin
Message: I have a researcher looking for a lab to do immunogold for calretinin on existing clinical epon812 blocks (Karnovskys, cacodylate buffer, osmium fixation, blocks cured at 70C).
Please reply to me directly (Peter.Steele-at-jhmi.edu).
Thank you.
Login Host: 198.57.41.129 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
Never say it won’t work before trying, but chances that this labeling would work would be slim in my opinion. I would suggest the researcher has a chat with people versed in this technique (there are quite a few that contribute to the list, may I recommend Hong Yi at Emory?). It is better to start off with specimens that might have a better chance of success. I would be happy to help as well.
And to all of you, have a great new year!
Jan Leunissen Aurion
} On 30/12/2017, at 12:06, microscopy.listserver-at-gmail.com wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: Peter.Steele-at-jhmi.edu } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } Peter.Steele-at-jhmi.edu as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: Peter.Steele-at-jhmi.edu Name: P. Steele } } Organization: JHACH } } Title-Subject: [Filtered] Immunogold Calretinin } } Message: I have a researcher looking for a lab to do immunogold for calretinin on existing clinical } epon812 blocks (Karnovskys, cacodylate buffer, osmium fixation, blocks cured at 70C). } } Please reply to me directly (Peter.Steele-at-jhmi.edu). } } Thank you. } } } } Login Host: 198.57.41.129 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 13, 53 -- From microscopy.listserver-at-gmail.com Fri Dec 29 17:05:56 2017 } 13, 53 -- Received: from mail-io0-f181.google.com (mail-io0-f181.google.com [209.85.223.181]) } 13, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id vBTN5uOU013633 } 13, 53 -- for {microscopy-at-microscopy.com} ; Fri, 29 Dec 2017 17:05:56 -0600 } 13, 53 -- Received: by mail-io0-f181.google.com with SMTP id x67so36265850ioi.9 } 13, 53 -- for {microscopy-at-microscopy.com} ; Fri, 29 Dec 2017 15:04:47 -0800 (PST) } 13, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 13, 53 -- d=gmail.com; s=20161025; } 13, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 13, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 13, 53 -- bh=TXcL5ha0564nMJ+8ZCajI9fZVXZ4wJ7LdB/4GpzMFt0=; } 13, 53 -- b=FMhCXTit+jAIVRavMek5Gw1LCzYncQxXL/bu6mWVQQ0zJqnA2KVtm5iHXCaSTQaR2M } 13, 53 -- QaP/F7MsLZErmKl2T6qyQVBVM/sqI7zHKJ15zLLPMO2oMskUYN4GxupKtfPkdLvud/7y } 13, 53 -- wiogQlarQMm8FvhQJwEgEJOqjh3EJ+HESF7HRMDEZ5AWfZugv2fzRP90HQEvfAnKyPWK } 13, 53 -- ok1pGS4ulXwNY+sFvfRV3+dQjYvKSY+8jDeGfpF7C8Oa+KdjccdDPzwTHOtuk+nYJKnQ } 13, 53 -- lUm3SZxT1tvMYHHPuaKuI0WWrFgDcPUwZ7/Hsb4FCLZ+cw16M/lKtxBnCt4LE6T3Cd1k } 13, 53 -- TeUg== } 13, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 13, 53 -- d=1e100.net; s=20161025; } 13, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 13, 53 -- :user-agent:mime-version:in-reply-to:content-language } 13, 53 -- :content-transfer-encoding; } 13, 53 -- bh=TXcL5ha0564nMJ+8ZCajI9fZVXZ4wJ7LdB/4GpzMFt0=; } 13, 53 -- b=gPzaJurM4G7ztGGyEWMG1Dkv+RsHdOzGDY411s33RzGh0www/zsPrfNmDi6+lBOOwV } 13, 53 -- /+clqiLhN+axbu2xrhH+VM4eGQ0oQi/VrP7k6yOvZTjpKM1HjIHdlz5OWsoGySAEAmr+ } 13, 53 -- 0MAl86E6S0zoJ4VQzXYtb7bTDcY6kPBF0PhJacQ6AksLSftBn73K+3pdRSaq4TfvIp62 } 13, 53 -- jmYBwY2G03UA9GlAXGFJHkcUGhZwaf22LWVdhYW3Bo2cX6Wb2nkX1b0BZ05WP0uM3bOi } 13, 53 -- XywVEkVegBkAB4A1tovYO0XYr/RXP1ehOPzveacdaigf553u9oAiZjrF4oi874av2G2t } 13, 53 -- YsgQ== } 13, 53 -- X-Gm-Message-State: AKGB3mIypg7AbSSlrmBoMBd0EiimeYrK1l/qI72VSCSCr3qJQ7bDIlVr } 13, 53 -- SNp63H91NH9NKpAQY1PzEkO1yzVv } 13, 53 -- X-Google-Smtp-Source: ACJfBotFiwzyztm4/6YcGZYr8d57EnExB4c15wYYcnO0yRSIj6vT9gzA2iUYYk3T1Ki56mSor22Pag== } 13, 53 -- X-Received: by 10.107.29.21 with SMTP id d21mr44000860iod.201.1514588686550; } 13, 53 -- Fri, 29 Dec 2017 15:04:46 -0800 (PST) } 13, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:2c8a:31c5:6676:7854]) } 13, 53 -- by smtp.googlemail.com with ESMTPSA id y35sm13501951ita.20.2017.12.29.15.04.43 } 13, 53 -- for {microscopy-at-microscopy.com} } 13, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 13, 53 -- Fri, 29 Dec 2017 15:04:44 -0800 (PST) } 13, 53 -- Subject: viaWWW:Immunogold Calretinin } 13, 53 -- References: {201712291833.vBTIXsFF031280-at-microscopy.com} } 13, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 13, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 13, 53 -- X-Forwarded-Message-Id: {201712291833.vBTIXsFF031280-at-microscopy.com} } 13, 53 -- Message-ID: {de4c8150-aedc-86f2-2178-fe1cde6fccb0-at-gmail.com} } 13, 53 -- Date: Fri, 29 Dec 2017 17:04:42 -0600 } 13, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) } 13, 53 -- Gecko/20100101 Thunderbird/52.5.2 } 13, 53 -- MIME-Version: 1.0 } 13, 53 -- In-Reply-To: {201712291833.vBTIXsFF031280-at-microscopy.com} } 13, 53 -- Content-Type: text/plain; charset=UTF-8; format=flowed } 13, 53 -- Content-Language: en-US } 13, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
From westelle638-at-gmail.com Sat Dec 30 12:22:35 2017 Return-Path: {westelle638-at-gmail.com} Received: from gmail.com (hn.kd.pix [219.155.154.249] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id vBUIMYG5013044 for {microscopylistserverarchive6-at-microscopy.com} ; Sat, 30 Dec 2017 12:22:35 -0600 Message-ID: {C78EFB49.6A9CE052-at-gmail.com}
MicroscopyListserver Archive Email Extraction Software Version NJZ07060908