Welcome to the another year of operation of the Microscopy ListServer a free service to the world wide microscopy community, sponsored jointly by your Friendly Neighborhood SysOp and the Microscopy Society of America.
During 2016, the ListServer delivered your messages to more than 4200 subscribers around the world, with minimal hassels (that I know about). For those of you that are statistics junkies this year you generated ~ 171+ Gbits of Email traffic and ~ 2.1 Million Email messages were sent out this year by my tired and old little server. Down abit from previous years, but still a steady flow.
This year I will be migrating the Listserver to a new server and ISP configuration so you might see a short outages during testing and transistions.
As usual you don't want to know how much Junk Mail and spam has been filtered out far more than you might expect. Apologies to those that have problems with my filters.
The complete Microscopy ListServer Archives for 2016-1994 (~ are on-line at
http://www.microscopy.com.
A couple of IMPORTANT reminders:
If you leave on vacation/holiday use the on-line form to UNSUBSCRIBE your Email address from the listserver. The out-of-office / on-vacation autoreply messages are a real nuisance to posters.
Do not reply to message with the return address of:
MicroscopyListserver-noreply-at-microscopy.com
these are messages forwarded usually from the WWW posting form. They do not go back to the poster but rather into a black hole, which I rarely check. If you see a message that has this "No-Reply" return address please post your reply/comment/answer to:
Microscopy-at-microscopy.com
or if you wish to reply privately, look at the username in the body of the message the originators Email address is usually listed therein.
As always if you have questions about suitability of postings or are having problems, feel free to contact me at (zaluzec-at-microscopy.com)
Cheers,
Nestor Your Friendly Neighborhood SysOp
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Does this go to archives now that I have fixed the forwarding system???
=========================================== Dr. Nestor J. Zaluzec Argonne National Laboratory Photon Sciences Division 9700 S. Cass Ave Bldg 212 / A-143 Argonne, Illinois 60439 USA Email: Zaluzec-at-aaem.amc.anl.gov
Tel: 530-NES-TORZ (530-637-8679) has Voice Mail Lab: 630-252-7901 Fax: 630-252-4798
Senior Scientist - Argonne National Laboratory Fellow of the Microscopy Society of America Senior Fellow the Computational Institute - University of Chicago E.P. Wigner Fellow - Oak Ridge National Laboratory Past President Microscopy Society of America Adjunct Professor of Physics - Northern Illinois University & the University of Illinois at Chicago Visiting Professor of Microscopy - Manchester University
Well my worst nightmares were realized on Monday Night (Jan 2) when I upgraded my server. Lots of my finely tuned filters and spam killers "broke". As such the Listserver has been down for ~ 2 days.
I believe all is fixed now. If you find/identify a problem please let me know.
Cheers,
Nestor Your Friendly Neighborhood SysOp
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Hi all,
Happy New Year!
Does anyone have a manual for a Sorvall Porter-Blum microtome, Type JB-4? If yes, can you please send me a copy?
best regards,
Beth Richardson
Georgia Electron Microscopy Lab
University of Georgia
Athens, GA
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both sbernstein-at-mail.sdsu.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: EM facility director position at SDSU
Message: Job Announcement: Electron Microscope Facility Lead (Instructional Support Technician III), College of Sciences - Department of Biology
Job ID:6232; San Diego State University Main Campus; Full-Time Regular
Please note: A completed application is required in order to be considered for this position.
About SDSU: San Diego State University is the oldest and largest higher education institution in the San Diego region. Since its founding in 1897, SDSU has grown to offer bachelor's degrees in 91 areas, master's degrees in 78 areas and doctorates in 22 areas. SDSU's approximately 35,000 students participate in an academic curriculum distinguished by direct contact with faculty and an increasing international emphasis that prepares them for a global future.
SDSU is a large, diverse, urban university and Hispanic-Serving Institution with a commitment to diversity, equity and inclusive excellence. Our campus community is diverse in many ways, including in terms of race, religion, color, sex, age, disability, marital status, sexual orientation, gender identity and expression, national origin, pregnancy, medical condition, and covered veteran status. We strive to build and sustain a welcoming environment for all. SDSU is an equal opportunity employer. SDSU is seeking applicants with demonstrated experience and/or commitment to teaching and working effectively with individuals from diverse backgrounds and members of underrepresented groups.
Position Information
Full-time, permanent (probationary) position. The incumbent is responsible for the day-to-day management of the SDSU Electron Microscope Facility, oversight of Biology common-use/shared research microscopes (other than EMs), collaborate with and train users of Biology research microscopes, keep EM Facility and Biology research microscope equipment funded and operational, order supplies, maintain EM Facility website. The incumbent also provides input and guidance on the microscopic needs of Biology lab courses.
Salary Range: Anticipated Hiring Salary Range: $4,107 - $6,473 per month (CSU Classification Salary Range: $4,107 - $6,667 per month). The competitive salary is determined by the education, experience, and qualifications the candidate brings to the position, internal equity, and the hiring department's fiscal resources.
Responsibilities: Training Biology and other SDSU faculty, staff and students in the theory and operation of EM Facility equipment and other Biology research microscopes Consulting with Biology and other SDSU faculty and students on research projects using light microscopy, transmission electron microscopy, and scanning electron microscopy Oversee external users of the facility and manage the fee-for-service accounting Collaborating and sharing expertise with Biology faculty and staff to incorporate EM Facility equipment and other Departmental microscope resources into the Biology curriculum Maintain equipment associated with EM Facility and Biology research microscopes, coordinate with repair providers; routine servicing of light microscopes used in biology courses Acquiring additional instrumentation to enlarge or improve the capabilities of the EM Facility and Biology research microscopes. This includes submitting grants, or assisting others in the preparation of grants, to obtain funds for new or updated instrumentation. Expanding the menu of microscopic techniques the Facility offers, through training and self-study Maintaining appropriate purchasing procedures, and tracking and billing users of facility Establishing outreach efforts to area schools and to the community, including designing and publishing the Facility Web site Representing the EM Facility and SDSU through service within professional organizations
Knowledge, Skills & Abilities: Knowledge of the principles and methods related to performing support services. Knowledge of the principles, information, methods and techniques related to discipline to which assigned. Knowledge of the materials and supplies related to the curriculum, their characteristics, and uses. Ability to plan, organize and schedule work; ability to operate and repair technical and scientific equipment. Ability to coordinate support service to meet a comprehensive variety of needs. Ability to develop off-campus resources related to the discipline for obtaining materials or equipment. Experience and Education
Equivalent to four years of experience providing instructional support services for a related unit or discipline, or in producing materials or supplies or repairing equipment in a discipline related to specialty area to which assigned.
OR
Equivalent to two years of college with 16 semester units in courses involving extensive use of materials, supplies, or equipment and in a discipline related to the area to which assigned may be substituted for one year of the required experience.
OR
Equivalent to four years of college with 16 semester units in courses involving extensive use of materials, supplies, or equipment and in a discipline related to the specialty area to which assigned may be substituted for two years of the required experience.
Specialized Requirements: Knowledge of the principles and methods related to light and electron microscopy Knowledge of the principles, information, methods and techniques related to biological sciences. Ability to coordinate support service to meet a comprehensive variety of needs of users. Ability to develop off-campus resources related to the operation, maintenance, and upgrading of Electron Microscopy Facility equipment Preferred Qualifications
Training/experience in operational design and theory of confocal/epifluorescent microscopy, light microscopy, scanning and transmission electron microscopy Experience in preparing samples for confocal/light microscopy, scanning and transmission electron microscopy Training/experience operating confocal/light microscopy, scanning and transmission electron microscopy Experience collecting and processing digital images Experience purchasing scientific equipment Relevant Masters degree Ph.D. in biological science Proven abilities in TEM and SEM operations as well as sample preparation techniques for these microscopes In addition to knowledge of the principles, information, methods and techniques related to biological sciences, it is preferable that individual also has knowledge in electron microscopy use in other scientific and engineering fields.
Application Procedures: Review of applications will begin on Wednesday, January 31, 2017; position will remain open until filled. The on-line application should be completed in detail. COMPLETION OF THE ONLINE APPLICATION IS REQUIRED FOR CONSIDERATION, A RESUME ALONE WILL NOT SUFFICE. Website for applying through the PeopleSoft system: https://cmsweb.cms.sdsu.edu/psp/HSDPRDF/EMPLOYEE/HRMS/c/HRS_HRAM.HRS_CE.GBL?Page=HRS_CE_HM_PRE&Action=A&SiteId=1
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As you start this new year, a reminder that this year's run of Analytical and Quantitative Light Microscopy, held at the Marine Biological Laboratory, Woods Hole, MA, USA, will run May 3 - May 12, 2017. Applications are due January 27, 2017.
The application is accessible via the course web site is at http://www.mbl.edu/aqlm. Financial assistance is available.
AQLM is a comprehensive and intensive course in light microscopy for researchers in biology, medicine, and material sciences. This course provides a systematic and in-depth examination of the theory of image formation and application of digital methods for exploring subtle interactions between light and the specimen. This course emphasizes the quantitative issues that are critical to the proper interpretation of images obtained with modern wide-field, confocal microscopes and new emerging technologies.
Laboratory exercises, demonstrations, and discussions include: • geometrical and physical optics of microscope image formation including Abbe's theory of the microscope and Fourier optics; • phase contrast, polarization and interference microscopy; • fluorescence microscopy, quantification of fluorescence, and fluorescent proteins; • principles and application of digital imaging and quantitative digital image deconvolution; • digital image processing and object identification and tracking; • live cell and ratiometric imaging for FRET and ion concentration imaging; • confocal microscopy and specialized methods such as TIRF and FLIM; and • new advances in light microscopy such as FCS, PALM, STORM, SIM and Light Sheet.
Within the course, we often refer to AQLM as "Microscopy Boot Camp". We cover every topic with a lecture and an in-depth hands-on lab. It's intense, it's hardcore, and it's really fun.
Why not join us for AQLM (or recommend one of your lab members or core facility users)? You'll never forget it.
Directors: Justin Taraska (National Institutes of Health), Jagesh Shah (Harvard Medical School) Course Laboratory Director: Wendy Salmon (Whitehead Institute/MIT)
Again, applications due January 27, 2017 and can be access through course web site at http://www.mbl.edu/aqlm.
Cheers, Jagesh, Justin and Wendy
Visit our Facebook page: http://www.facebook.com/aqlmcourse Follow us on Twitter: -at-BeadsinMay
Wendy
~~~~~~~~~~~~~~~~~~~~~~~ Wendy Salmon Light Microscopy Specialist Whitehead Institute for Biomedical Research W.M. Keck Imaging Facility **STREET ADDRESS CHANGE** 455 Main St, Rm 447 Cambridge, MA 02142 c: 617-429-0158 e: wsalmon-at-wi.mit.edu w: http://staffa.wi.mit.edu/microscopy/
==============================Original Headers============================== 16, 41 -- From wsalmon-at-wi.mit.edu Thu Jan 5 07:08:11 2017 16, 41 -- Received: from ptolemy.wi.mit.edu (ptolemy.wi.mit.edu [18.4.1.120]) 16, 41 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v05D8BfU027039 16, 41 -- for {microscopy-at-microscopy.com} ; Thu, 5 Jan 2017 07:08:11 -0600 16, 41 -- X-IronPort-AV: E=Sophos;i="5.33,321,1477972800"; 16, 41 -- d="scan'208";a="11308408" 16, 41 -- Received: from unknown (HELO mars.wi.mit.edu) ([10.9.4.20]) 16, 41 -- by ptolemy.wi.mit.edu with ESMTP; 05 Jan 2017 08:12:34 -0500 16, 41 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 16, 41 -- by mars.wi.mit.edu (Postfix) with ESMTP id C0BA431EDCE 16, 41 -- for {microscopy-at-microscopy.com} ; Thu, 5 Jan 2017 08:12:34 -0500 (EST) 16, 41 -- Received: from mars.wi.mit.edu ([127.0.0.1]) 16, 41 -- by localhost (mars.wi.mit.edu [127.0.0.1]) (amavisd-new, port 10032) 16, 41 -- with ESMTP id 2_j5h33ilqTW for {microscopy-at-microscopy.com} ; 16, 41 -- Thu, 5 Jan 2017 08:12:34 -0500 (EST) 16, 41 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 16, 41 -- by mars.wi.mit.edu (Postfix) with ESMTP id 3C06D31EDD2 16, 41 -- for {microscopy-at-microscopy.com} ; Thu, 5 Jan 2017 08:12:34 -0500 (EST) 16, 41 -- X-Virus-Scanned: amavisd-new at mars.wi.mit.edu 16, 41 -- Received: from mars.wi.mit.edu ([127.0.0.1]) 16, 41 -- by localhost (mars.wi.mit.edu [127.0.0.1]) (amavisd-new, port 10026) 16, 41 -- with ESMTP id hR_rowKutR4r for {microscopy-at-microscopy.com} ; 16, 41 -- Thu, 5 Jan 2017 08:12:34 -0500 (EST) 16, 41 -- Received: from io.wi.mit.edu (io.wi.mit.edu [10.9.4.21]) 16, 41 -- by mars.wi.mit.edu (Postfix) with ESMTP id 2250B31EDCE 16, 41 -- for {microscopy-at-microscopy.com} ; Thu, 5 Jan 2017 08:12:34 -0500 (EST) 16, 41 -- Date: Thu, 5 Jan 2017 08:12:33 -0500 (EST) 16, 41 -- From: Wendy Salmon {wsalmon-at-wi.mit.edu} 16, 41 -- To: microscopy {microscopy-at-microscopy.com} 16, 41 -- Message-ID: {2076351021.11730088.1483621953838.JavaMail.zimbra-at-wi.mit.edu} 16, 41 -- In-Reply-To: {1563420676.11456151.1483569237344.JavaMail.zimbra-at-wi.mit.edu} 16, 41 -- References: {1563420676.11456151.1483569237344.JavaMail.zimbra-at-wi.mit.edu} 16, 41 -- Subject: 2nd Announcement: Analytical and Quantitative Light Microscopy 2017 16, 41 -- MIME-Version: 1.0 16, 41 -- Content-Type: text/plain; charset=utf-8 16, 41 -- X-Originating-IP: [10.9.41.56] 16, 41 -- X-Mailer: Zimbra 8.6.0_GA_1191 (ZimbraWebClient - GC55 (Mac)/8.6.0_GA_1191) 16, 41 -- Thread-Topic: 2nd Announcement: Analytical and Quantitative Light Microscopy 2017 16, 41 -- Thread-Index: YkApMdUfqeWYfhn8ozABZ9snK8fNPH2VKVPL 16, 41 -- Content-Transfer-Encoding: 8bit 16, 41 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v05D8BfU027039 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ravi.thakkar369-at-gmail.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: ravi.thakkar369-at-gmail.com Name: Ravi
Title-Subject: [Filtered] EDS report shows Boron, even its Boron peak is not present in spectrum.
Message: Hi Listeners, I am using Oxford EDS (SiLi) on Hitachi S 3500N SEM. Surprisingly, I am getting false Boron atomic% count.(some times values are in negative) in spectrum report without any detectable peak in the spectrum. Here I am using Inca 5.05 I am not able to remove the Boron from the element count table by confirm elements options because absence of boron peak.
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both gary-at-microtechnics.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: gary-at-microtechnics.com Name: Dr Gary Gaugler
Organization: Microtechnics
Title-Subject: [Filtered] BSD for Zeiss Supra 40
Message: Hi All:
Does anyone know of BSD options (scintillator or YAG) for the Zeiss Supra 40? Robinson has retired. A standard Deben Centaurus does not fit a chamber port without a large extra cost.
Any ideas or suggestions are appreciated.
gary g.
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ravi.thakkar369-at-gmail.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: ravi.thakkar369-at-gmail.com Name: Rav
Title-Subject: [Microscopy] viaWWW: EDS report shows Boron, even its Boron peak is not present.
Message: I am having boron counts even its absent in confirm elements. Here, I am using Oxford EDS on Hitachi SEM using Inca 5.05 Hope that image file linked to following link will helpful to you to understand the issue.
At AgResearch, NZ's Government owned agricultural research institute, we have two microscopy related positions with closing dates in the next few weeks (27th Jan for scientist position, and 20th Jan for PhD scholarship).
These should be of interest to people interested the structure/biology of hair and wool (hard-keratin materials).
The scientist position is aimed at an early career scientist who has completed a PhD and has good team skill with a track record in innovative science.
The PhD scholarship focuses on location of hair-specific proteins within the developing fibre using high-pressure freezing and immunolabelling.
Full details of the two positions can be found on AgResearch's website (www.agresearch.co.nz/careers) and there are also links from AgResearch's Linked In jobs page.
Duane
____________________________ Dr Duane P Harland Senior Scientist T +64 3 321 8710 E duane.harland-at-agresearch.co.nz AgResearch Limited Lincoln Research Centre Cnr Springs Rd & Gerald Street, Lincoln Private Bag 4749 Christchurch 8140, New Zealand T +64 3 325 9900 F +64 3 325 9946 www.agresearch.co.nz ======================================================================= Attention: The information contained in this message and/or attachments from AgResearch Limited is intended only for the persons or entities to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipients is prohibited by AgResearch Limited. If you have received this message in error, please notify the sender immediately. =======================================================================
==============================Original Headers============================== 9, 37 -- From Duane.Harland-at-agresearch.co.nz Sun Jan 8 15:31:37 2017 9, 37 -- Received: from out1103.nz.smxemail.com (out1103.nz.smxemail.com [203.84.134.34]) 9, 37 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v08LVaww010502 9, 37 -- for {microscopy-at-microscopy.com} ; Sun, 8 Jan 2017 15:31:36 -0600 9, 37 -- DKIM-Signature: v=1; a=rsa-sha256; d=nz.smxemail.com; s=alpha; c=relaxed/relaxed; 9, 37 -- q=dns/txt; i=-at-nz.smxemail.com; t=1483911371; 9, 37 -- h=From:Sender:Reply-To:Subject:Date:Message-ID:To:Cc; 9, 37 -- bh=RC1+BGNpD3idI0P9C8PWETtzsGO4AvHoIP2vg/yZqZ0=; 9, 37 -- b=htfz+2+qoNdPKgBlgVEXSxDWcnWGJyeKxr0zYfF1E6QYdTk5KomQLo4EcymU1nWh 9, 37 -- tJnBdZzY8eEYvd8Cx/2zEOKmL5QsAnRZs1sKebcDsLdyL2a9Z1exCdSWxT7Ze2hU 9, 37 -- ApMBA9qNUOqrIYEUatWeQd0Njyf1QLSYtiPpKWFZbVs=; 9, 37 -- Received: from invexcpv02.agresearch.co.nz ([202.20.102.50]) 9, 37 -- by omr.nz.smxemail.com with ESMTP (using TLSv1 9, 37 -- with cipher AES128-SHA (128/128 bits)) 9, 37 -- id 5872B0CB-135A0D6A-at-mta1104.omr; 9, 37 -- Sun, 08 Jan 2017 21:36:11 +0000 9, 37 -- Received: from INVEXCPV01.agresearch.co.nz ([147.158.128.23]) by 9, 37 -- invexcpv02.agresearch.co.nz ([147.158.130.77]) with mapi id 14.03.0294.000; 9, 37 -- Mon, 9 Jan 2017 10:36:07 +1300 9, 37 -- From: "Harland, Duane" {Duane.Harland-at-agresearch.co.nz} 9, 37 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 9, 37 -- Subject: EM scientist and PhD scholarship on immunolabelling TEM in New 9, 37 -- Zealand 9, 37 -- Thread-Topic: EM scientist and PhD scholarship on immunolabelling TEM in New 9, 37 -- Zealand 9, 37 -- Thread-Index: AQHSafWipn+d/TiqikWdZ6kPnec4rA== 9, 37 -- Date: Sun, 8 Jan 2017 21:36:07 +0000 9, 37 -- Message-ID: {C1CB2BCA7689064DA55A366147C8BEF1013DB42233-at-invexcpv01.agresearch.co.nz} 9, 37 -- Accept-Language: en-GB, en-NZ, en-US 9, 37 -- Content-Language: en-GB 9, 37 -- X-MS-Has-Attach: 9, 37 -- X-MS-TNEF-Correlator: 9, 37 -- x-originating-ip: [202.20.103.209] 9, 37 -- Content-Type: text/plain; charset="iso-8859-1" 9, 37 -- MIME-Version: 1.0 9, 37 -- Content-Transfer-Encoding: 8bit 9, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v08LVaww010502 ==============================End of - Headers==============================
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Email: m.aindow-at-uconn.edu Name: Mark Aindow
Organization: University of Connecticut
Title-Subject: [Filtered] Postdoctoral Positions in the UConn-FEI Center for Advanced Microscopy and Materials Analysis
Message: The University of Connecticut (UConn) has partnered with FEI to establish the Center for Advanced Microscopy and Materials Analysis (CAMMA), which includes a suite of seven new electron- and ion-beam instruments. CAMMA will form part of the state-of-the-art Advanced Characterization Laboratories in the UConn Technology Park, which is currently under construction and will open later this year. Five of the new instruments are currently housed in the Institute of Materials Science at UConn and will be relocated to the new Technology Park facility later in 2017. There are two CAMMA postdoctoral positions available immediately: 1. 3D studies of complex microstructures using Xe plasma FIB. This position will involve the development and exploitation of serial sectioning and data reconstruction strategies using the FEI Helios plasma FIB. Candidates should have a good working knowledge of FIB and electron microscopy techniques, including EDXS and EBSD. A clear understanding of microstructural issues in metallic alloys, ceramics and coatings is important for this post. 2. Microstructural development in aerospace alloys. This position will involve working with local aerospace industries on a range of projects relating to microstructural issues in Ni, Ti and Al alloys, and in thermal barrier coatings. Candidates should have extensive experience with relevant materials systems and with a broad range of electron microscopy techniques. Due to the nature of some of the projects, only US citizens or permanent residents will be considered for this second position.
Both of these positions are for one year in the first instance, with the possibility of renewal. To apply, please send a resume, together with a full list of publications, a statement of relevant experience, and contact details for three references to Prof. Mark Aindow (m.aindow-at-uconn.edu). The University of Connecticuts commitment to excellence is complemented by our commitment to building a culturally diverse community. We encourage applications from under-represented groups, including minorities, women, and people with disabilities. The University of Connecticut is an equal opportunity employer and program provider. Screening of applications will begin immediately and will continue until the positions are filled.
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From steven.345345.f-at-gmail.com Tue Jan 10 11:19:33 2017 Return-Path: {steven.345345.f-at-gmail.com} Received: from gmail.com ([106.249.239.99]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v0AHJUvT029050 for {microscopylistserverarchive6-at-microscopy.com} ; Tue, 10 Jan 2017 11:19:32 -0600 Message-ID: {6C886BB8.CC131669-at-gmail.com}
X-from: vakimler-at-oakland.edu
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Organization: Oakland University Eye Research Institute
Title-Subject: [Filtered] LR White Ultrathin Sections Prepared for ImmunoGold
Message: How long (in terms of days) in advance can I cut ultrathin sections (usually 70-90 nm) in preparation for immunogold applications? The resin was medium hardness with catalyst, and I polymerized it in a 55 degree oven overnight.
I was wondering if the surface of the ultrathin section would be altered over a few days after cutting and antibodies would be somewhat hindered from access to epitopes.
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*********************************************************************************** Forwarded from "Ask a Microscopist" Please remember that the person asking the question is likely not a member the listserver, and **any reply should go directly to the poster** as well as to the list. Using the "reply" function in your email does *not* send your answer to the person asking the question. Please copy their email address from their question. *********************************************************************************** Ask a Microscopist
The following Ask a Microscopist form submission was received from your website.
Name: Jacar Brocker School: Delft University of Technology Grade/Education Level: Graduate Location: Delft, NL Email: j.h.brocker-at-student.tudelft.nl
X-from: jpshield-at-uga.edu
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Email: jpshield-at-uga.edu Name: John Shields
Organization: University of Georgia
Title-Subject: [Filtered] Bio TEM Workshop -
Message: Biological TEM Workshop
This intensive, three-day workshop will provide a practical and basic theoretical introduction to the Transmission Electron Microscope and biological sample preparation techniques. Each day will consist of lecture, discussion and hands-on training led by GEM staff. What: Anyone requiring training on TEM and biological sample preparation. The workshop will be limited to 6 participants based on the availability of equipment. When: Monday through Wednesday, March 8-10, 2017, 8am-5pm each day (lunch is provided)
Where: 115 D.E. Brooks Drive, 154 Barrow Hall, University of Georgia, Athens, GA 30602
Registration: Contact John Shields (jpshield-at-uga.edu) for more information and to sign up. Registration requires iLab account through the GEM website. https://uga.ilabsolutions.com/account/login
Deadline: February 27, 2017
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Email: Ursel.Bangert-at-ul.ie Name: Ursel Bangert
Organization: University of Limerick
Title-Subject: [Filtered] Invitation to submit abstracts to the Celtic Sessions at mmc2017
Message: Microcscience Microscopy Congress 2017 Celtic Sessions
The Microscopy Society of Ireland (MSI) is co-organising two sessions (together with the Scottish Microscopy Group, SMG) at the Microscience Microscopy Congress (mmc2017), organised by the Royal Microscopical Society. The event will take place in Manchester, UK, from July 3rd-6th. The international mmc2017 conference will comprise over 30 symposia, with excellent speakers and vibrant supporting poster sessions. Further to presenting new research in Ireland and Scotland, the Celtic Sessions are aimed at showcasing and encouraging collaborative research, and all members of the international microscopy community are sincerely invited to contribute to the Celtic Sessions, which will include invited and regular talks, as well as flash presentations and poster sessions. The abstract submission for mmc2017 is now open. We are inviting abstracts for the Celtic Sessions: Biological Processes at the Nanoscale Inorganic Nanomaterials For more information about how to participate and submit abstracts, please go to the MSI website http://microscopy.ie/ or http://microscopy.ie/2017.php, or submit your abstract directly through the mmc website http://mmc-series.org.uk/about/news/abstract-submission-now-open or through the official mmc2017 flyer http://microscopy.ie/Doc/MSI_Call_for_Papers_Flyer2.pdf.
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} Dear sir/madam, For a masters course we have to find the market size for a planar positioning stage. We are having trouble finding sales numbers or estimating how many microscopists use an automatic positioning stage with their microscope or how many would like to use one if the price is right. Hopefully you could provide us with some global estimates. Kind regards, Jacar Brocker (4079450) }
Global estimates?
Does asking someone else to do the research work for one's degree allow one to earn it, I wonder?
And a Masters in marketing at that.
Ask A Microscopist may be a reasonable start, though asking around the University would probably work even better.
Find someone who may use or be interested in product and talk with them. When you have learned what you and they want to learn, ask if they know someone else with whom you can talk. Repeat the above until you have learned enough.
No number of degrees, doctorates or whatever will avoid that. If you want to succeed at sales, marketing, engineering, science and a number of other disciplines, you must ignore the knot in your stomach, ignore the fear of failure, embarrassment or humiliation, get out there and talk to people. In practice, most of the time there will not be failure, embarrassment or humiliation. Generally, people will be pleased you take an interest. For many people, a coffee, croissant and 20 minute break from work makes a nice change, too.
An ex colleague of mine used sometimes to come back from meetings with potential or actual customers, suppliers, partners or whatever and use the term "WoFT". If one meets with people in those circumstances it is for each party to learn, even if what one learns is that nobody wants the product. IMHO, the only party to whom "WoFT" can apply is oneself.
Gordon.
==============================Original Headers============================== 13, 20 -- From gordon-at-gscott.co.uk Thu Jan 12 04:52:39 2017 13, 20 -- Received: from osmia.gscott.co.uk (87-127-155-63.static.enta.net [87.127.155.63]) 13, 20 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0CAqc5k027778 13, 20 -- for {microscopy-at-microscopy.com} ; Thu, 12 Jan 2017 04:52:39 -0600 13, 20 -- Received: from [10.0.0.101] (CoolLED-PC.gscott.co.uk [10.0.0.101]) 13, 20 -- by osmia.gscott.co.uk (Postfix) with ESMTPS id 9CCFBDF9EA; 13, 20 -- Thu, 12 Jan 2017 10:57:00 +0000 (GMT) 13, 20 -- Subject: Re: [Microscopy] Ask a Microscopist - marketing question re: planar 13, 20 -- positioning stages 13, 20 -- References: {201701112208.v0BM8bSj015503-at-microscopy.com} 13, 20 -- To: microscopy-at-microscopy.com, j.h.brocker-at-student.tudelft.nl 13, 20 -- From: Gordon Scott {gordon-at-gscott.co.uk} 13, 20 -- Message-ID: {522dd9b3-bfb3-9cd4-441d-7836eccd4e46-at-gscott.co.uk} 13, 20 -- Date: Thu, 12 Jan 2017 10:57:00 +0000 13, 20 -- User-Agent: Mozilla/5.0 (X11; Linux x86_64; rv:45.0) Gecko/20100101 13, 20 -- Thunderbird/45.5.1 13, 20 -- MIME-Version: 1.0 13, 20 -- In-Reply-To: {201701112208.v0BM8bSj015503-at-microscopy.com} 13, 20 -- Content-Type: text/plain; charset=windows-1252; format=flowed 13, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
From conversion.seo-at-gmail.com Thu Jan 12 06:26:21 2017 Return-Path: {conversion.seo-at-gmail.com} Received: from gmail.com ([106.249.239.99]) by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0CCQHPP006958 for {microscopylistserverarchive6-at-microscopy.com} ; Thu, 12 Jan 2017 06:26:19 -0600 Reply-To: conversion.seo-at-gmail.com charset="utf-8" Content-Transfer-Encoding: 8bit X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v0CCQHPP006958
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Organization: Florida State University
Title-Subject: Postdoc position available
Job Title: Post-Doctoral Associate - Electron Microscopist
Summary Description:
Florida State University (FSU) and National High Magnetic Field Laboratory (NHMFL) invite applications for a post-doctoral research associate position to undertake research projects led by Prof. Fumitake Kametani. The research projects aim to understand the nano- and atomic-structural correlations to the material properties by using the advanced electron microscopes including aberration-corrected atomic resolution (scanning) transmission electron microscope (S/TEM) and focused ion beam (FIB). A person who has an experience in the atomic and nano-structural analyses of complicated materials such as high temperature superconductors, carbon nanotube composites, perovskite thin films or energy-related materials is encouraged to apply. This position requires expertise in atomic resolution imaging in a TEM/STEM and nano-fabrication by using a FIB. As listed below, we already have the state-of-art electron microscopes here, so he/she is expected to start working material problems right away.
This position is a full time position with an anticipated starting salary at a minimum of $48,000 and includes health benefits.
Major microscope-related instruments at FSU and NHMFL: * JEOL ARM200cF Cs-corrected S/TEM with Electron Energy Loss Spectroscopy (EELS) and Energy-dispersive X-ray Spectroscopy (EDS) * Carl Zeiss 1540EsB field emission scanning electron microscope and FIB equipped with Gas Injection System and OmniProbe in-situ lift-out tool * Gatan PIPS ion mill * Micro Support Axis Pro SS ex-situ lift-out tool * JEOL IB-19500 CP ion cross section polisher
Requirements: * Ph.D. in materials science, physics, engineering, chemistry, or related field. * Must have experience to operate TEM. * Must have experience preparing specimens using FIB. * Preferred to have experience operating Cs-corrected STEMs. * Preferred to have experience in EELS.
Please directly send CV including 2 references to:
Fumitake Kametani Associate Professor Department of Mechanical Engineering, Florida State University, and Applied Superconductivity Center, National High Magnetic Field Laboratory 2031 E. Paul Dirac Dr., Tallahassee, FL 32310 Tel: (850) 645-7491 E-mail: fkametani-at-fsu.edu
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From rosaronald70-at-gmail.com Sat Jan 14 23:53:38 2017 Return-Path: {rosaronald70-at-gmail.com} Received: from gmail.com ([183.111.66.21]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v0F5rZW6022865 for {microscopylistserverarchive6-at-microscopy.com} ; Sat, 14 Jan 2017 23:53:36 -0600 Message-ID: {EF2986AB.D798EF4F-at-gmail.com}
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Title-Subject: [Filtered] LR White Ultrathin Sections Prepared for ImmunoGold
Message: Hello, Second Request - Not sure if the first message was transmitted... What is the maximum time I need to wait in order to apply immunogold reagents (buffers, etc.) to ultrathin sections of LR White blocks (Medium hardness with catalyst? Thanks much, Vickie
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I do not think there is such a thing as a maximum time you have to wait before which LR white sections would be suited for immuno. Perhaps you are wondering if there is a minimum time before sections are suited? As far as our experience goes LR-white and Lowicryl sections seem to keep pretty much indefinitely. Perhaps it would be wise if they were kept in a dessicator. I do not think there is a minimum time one should consider having to wait. If things change post-sectioning I would expect epitopes to change (oxidize?) and that hardly ever is beneficial. And how would ‘buffers etc’ relate to that, I do not understand?
Are you getting results, or not getting results, that make you wonder whether section age plays a role?
If you like, please feel free to get in touch off-list. I will be happy to help.
Jan Leunissen - - - Aurion - ImmunoGold Reagents www.aurion.nl
==============================Original Headers============================== 9, 35 -- From leunissen-at-aurion.nl Mon Jan 16 18:20:18 2017 9, 35 -- Received: from nm11-vm2.access.bullet.mail.bf1.yahoo.com (nm11-vm2.access.bullet.mail.bf1.yahoo.com [216.109.114.225]) 9, 35 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0H0KH0V020760 9, 35 -- for {microscopy-at-microscopy.com} ; Mon, 16 Jan 2017 18:20:18 -0600 9, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1484612696; bh=6gxrqluLWgQ7XyCrQJ7BDd0QDfMuuFqjUzs7pf/1nxE=; h=Subject:From:In-Reply-To:Date:Cc:References:To:From:Subject; b=kMdPBGVy6G+HOV9lPFVb4PgI48Yaf1zMZs/qhVJlhe1mYsj8SgGrv4R+9VFWhxTl4AXTntPEEyuX7jcLbMZDz0uBKcyknpEt0eqTTeJqqi04gD/Y5oMmdUE2SkEG10xPAP8ydlaMFThXqkGxWxilnXTOdSCxB497xPPtiiW2Uagsgl8jK05bcIejeYoVBrq+a2Ur5WQ4wZk3r12QScSPcyaXI93FeJBroLyQEAZLD7Lez8C0IT1j5MtA/p859/AcUqMf5Qr6Z628vrVEkP/1GxZUhTjUlkICXmZnQD6/2JFf/K2K82pbz3w9EqhHONkCxt5zrU4jjiiEQ6mahRAYdA== 9, 35 -- Received: from [66.196.81.159] by nm11.access.bullet.mail.bf1.yahoo.com with NNFMP; 17 Jan 2017 00:24:56 -0000 9, 35 -- Received: from [98.138.226.240] by tm5.access.bullet.mail.bf1.yahoo.com with NNFMP; 17 Jan 2017 00:24:56 -0000 9, 35 -- Received: from [127.0.0.1] by smtp111.sbc.mail.ne1.yahoo.com with NNFMP; 17 Jan 2017 00:24:55 -0000 9, 35 -- X-Yahoo-Newman-Id: 942867.29936.bm-at-smtp111.sbc.mail.ne1.yahoo.com 9, 35 -- X-Yahoo-Newman-Property: ymail-3 9, 35 -- X-YMail-OSG: iqgaVIAVM1ldH49kVDcXX4XZXLCeGJtjQATeJsFWXvO.JBY 9, 35 -- 1SWioi7Ux9IAYWBNZqP1eW.VEzqa9y1fQOkwZ9xiBnTAmwD6RFFzwZtXT9dp 9, 35 -- sxi1eOwiR5d32oC5pOMSvBq_Prb_xPw89Nhrr7VfQNPyD2UHaJg36KHtS1OT 9, 35 -- FTz5NwM9mNIGgQM0cihMy1QYy9ERz6b8R5LNYIWS729LnB0KtkqBvqqkLnQs 9, 35 -- AxUvAfTXvBAegyZDlSjf_pXwyZGUSX0.dK6rH9luGqD_agTJF69X33dZH.lU 9, 35 -- 5r4jN9cSEGwX.6D7uHdsm040PfzEEF.4IgZNLsAYkWsTdDeEJznGmrdbEQZi 9, 35 -- fr8mq3wxmmBpyZgLo6Zzy0TMYphcd0q8vgF3hSvhiDzaD0rtmQQUXIsrr8h8 9, 35 -- 6DZgZiQqwzwOpPRmiHjrLV1IviBUxx0rm9S810tg_LCdvHbaP5YXJXNTe9lo 9, 35 -- _mcoVFPwgbuJM_GTbyV2aYhxaUqbe4sU4lbT7t_7BwMSaQQDRmcUnhgvh0Ul 9, 35 -- LpYRd7Rykj9X2noDo3y_QbI6ozWBMxccf55limj0e8UW9WKCmemPgYkhDaDa 9, 35 -- 1I6uBWazirBg1Pw-- 9, 35 -- X-Yahoo-SMTP: E1TINkOswBCOniPaNnp23qBGQpLN4KsU.cr0KdlJlWhF 9, 35 -- Content-Type: text/plain; charset=utf-8 9, 35 -- Mime-Version: 1.0 (Mac OS X Mail 9.3 \(3124\)) 9, 35 -- Subject: Re: [Microscopy] viaWWW:LR White Ultrathin Sections Prepared for ImmunoGold 9, 35 -- From: Jan Leunissen {leunissen-at-aurion.nl} 9, 35 -- In-Reply-To: {201701162359.v0GNxklD002178-at-microscopy.com} 9, 35 -- Date: Tue, 17 Jan 2017 13:24:49 +1300 9, 35 -- Cc: microscopy-at-microscopy.com 9, 35 -- Message-Id: {50900377-4A4C-4BB3-9AF6-608B6A1B2058-at-aurion.nl} 9, 35 -- References: {201701162359.v0GNxklD002178-at-microscopy.com} 9, 35 -- To: vakimler-at-oakland.edu 9, 35 -- X-Mailer: Apple Mail (2.3124) 9, 35 -- Content-Transfer-Encoding: 8bit 9, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v0H0KH0V020760 ==============================End of - Headers==============================
Here's a story: Someone once had LR-White sections that were more than two years old, from another institution. They went ahead and applied their antibody, secondary antibody and gold, and seemed to have every expectation that it would work (whether based on experience or blind faith, I do not know). However, they needed images immediately for a grant proposal, or at least they needed to know if a particular protein was on a particular organ in the squid. My TEM (the only one in the state) was down. In a panic, we stuck them in the SEM wehere we could see the gold particles on the surface of the section, and we could also see outlines of the ultrastructure because parts of the tissue had bulged out of the resin enough to give some relief. In fact, it looked really cool. And they were able to state that the protein was in that organ. If you've ever sectioned a block and then let it sit for some time (hours, years?), and than sectioned again you've probably noticed that some tissues expand out further than others over time.
Anyway, in this case I'm sure their antibody would have worked right away on freshly-cut sections as well as on sections that had been sitting on grids for more than two years. The resin may flow somewhat over time. Your mileage may vary. Perform a bunch of timing experiments and report back!
Aloha, Tina
} Email: vakimler-at-oakland.edu Name: Vickie Kimler } } Organization: Eye Research Institute } } Title-Subject: [Filtered] LR White Ultrathin Sections Prepared for ImmunoGold } } Message: Hello, } Second Request - Not sure if the first message was transmitted... } What is the maximum time I need to wait in order to apply immunogold reagents } (buffers, etc.) to } ultrathin sections of LR White blocks (Medium hardness with catalyst? } Thanks much, } Vickie }
==============================Original Headers============================== 6, 21 -- From tina-at-pbrc.hawaii.edu Mon Jan 16 19:27:16 2017 6, 21 -- Received: from b1000.pbrc.hawaii.edu (b1000.pbrc.hawaii.edu [128.171.22.30]) 6, 21 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0H1RFg6016186 6, 21 -- for {Microscopy-at-microscopy.com} ; Mon, 16 Jan 2017 19:27:16 -0600 6, 21 -- Received: from b1000.pbrc.hawaii.edu (localhost [127.0.0.1]) 6, 21 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id v0H1VqXn012379 6, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 6, 21 -- for {Microscopy-at-microscopy.com} ; Mon, 16 Jan 2017 15:31:53 -1000 (HST) 6, 21 -- Received: from localhost (tina-at-localhost) 6, 21 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP id v0H1Vqft012375 6, 21 -- for {Microscopy-at-microscopy.com} ; Mon, 16 Jan 2017 15:31:52 -1000 (HST) 6, 21 -- X-Authentication-Warning: b1000.pbrc.hawaii.edu: tina owned process doing -bs 6, 21 -- Date: Mon, 16 Jan 2017 15:31:52 -1000 (HST) 6, 21 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 6, 21 -- X-X-Sender: tina-at-b1000 6, 21 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 6, 21 -- Subject: Re: [Microscopy] viaWWW:LR White Ultrathin Sections Prepared for 6, 21 -- ImmunoGold 6, 21 -- Message-ID: {Pine.GSO.4.64.1701161529260.12266-at-b1000} 6, 21 -- MIME-Version: 1.0 6, 21 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
Hello, I wanted to know if anyone has had experience using curtain wall systems to segment a space for microscopes without building hard walls. I want to make a somewhat-temporary home for an instrument. Laser safety curtaining is common but way too expensive and unnecessary, since we have no lasers. Has anyone used any of the industrial-type curtain systems to divide up a space? Thanks for any information.
Regards, Larry Scipioni ZS Genetics
==============================Original Headers============================== 3, 52 -- From les-at-zsgenetics.com Tue Jan 17 13:27:06 2017 3, 52 -- Received: from gateway21.websitewelcome.com (gateway21.websitewelcome.com [192.185.45.155]) 3, 52 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0HJR5Mm018712 3, 52 -- for {microscopy-at-microscopy.com} ; Tue, 17 Jan 2017 13:27:06 -0600 3, 52 -- Received: from cm3.websitewelcome.com (unknown [108.167.139.23]) 3, 52 -- by gateway21.websitewelcome.com (Postfix) with ESMTP id 3EC58400D9595 3, 52 -- for {microscopy-at-microscopy.com} ; Tue, 17 Jan 2017 13:31:45 -0600 (CST) 3, 52 -- Received: from gator2013.hostgator.com ([50.87.144.13]) 3, 52 -- by cm3.websitewelcome.com with 3, 52 -- id ZXXj1u00y0HZY1u01XXkjj; Tue, 17 Jan 2017 13:31:45 -0600 3, 52 -- DKIM-Signature: v=1; a=rsa-sha256; q=dns/txt; c=relaxed/relaxed; 3, 52 -- d=zsgenetics.com; s=default; h=Content-Transfer-Encoding:Content-Type: 3, 52 -- MIME-Version:Message-ID:Date:Subject:To:From:Sender:Reply-To:Cc:Content-ID: 3, 52 -- Content-Description:Resent-Date:Resent-From:Resent-Sender:Resent-To:Resent-Cc 3, 52 -- :Resent-Message-ID:In-Reply-To:References:List-Id:List-Help:List-Unsubscribe: 3, 52 -- List-Subscribe:List-Post:List-Owner:List-Archive; 3, 52 -- bh=w1HMFSQ3qjgKsehRQSv57Jy5dMB1VmzALc2p8oqZTzI=; b=mM2CBSECySlrLyx65O6NEROl40 3, 52 -- xlUFwWR0RFfRGCO1lE9WexzfrjiaOYpqtYp9VLI/KVf11VpcvmN/8NdAUz66Q9H376bm4cm41EFol 3, 52 -- oe+oqQ9bn9Iqv1MUjZAA9be0gTdfGIilVRE4PBAh6KewVtKF6rO0ZxXXwZO3Z2KZi+BA=; 3, 52 -- Received: from zsgenetics03.z.subnet.rcn.com ([146.115.5.114]:50951 helo=LarryPC) 3, 52 -- by gator2013.hostgator.com with esmtpsa (TLSv1.2:ECDHE-RSA-AES256-GCM-SHA384:256) 3, 52 -- (Exim 4.87) 3, 52 -- (envelope-from {les-at-zsgenetics.com} ) 3, 52 -- id 1cTZTr-000KOX-3Z 3, 52 -- for microscopy-at-microscopy.com; Tue, 17 Jan 2017 13:31:43 -0600 3, 52 -- From: {les-at-zsgenetics.com} 3, 52 -- To: {microscopy-at-microscopy.com} 3, 52 -- Subject: curtain systems 3, 52 -- Date: Tue, 17 Jan 2017 14:31:41 -0500 3, 52 -- Message-ID: {042501d270f8$5522a5a0$ff67f0e0$-at-zsgenetics.com} 3, 52 -- MIME-Version: 1.0 3, 52 -- Content-Type: text/plain; 3, 52 -- charset="us-ascii" 3, 52 -- Content-Transfer-Encoding: 7bit 3, 52 -- X-Mailer: Microsoft Outlook 16.0 3, 52 -- Thread-Index: AdJw9zrv+yTN6KMwQkegivozoJS38w== 3, 52 -- Content-Language: en-us 3, 52 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report 3, 52 -- X-AntiAbuse: Primary Hostname - gator2013.hostgator.com 3, 52 -- X-AntiAbuse: Original Domain - microscopy.com 3, 52 -- X-AntiAbuse: Originator/Caller UID/GID - [47 12] / [47 12] 3, 52 -- X-AntiAbuse: Sender Address Domain - zsgenetics.com 3, 52 -- X-BWhitelist: no 3, 52 -- X-Source-IP: 146.115.5.114 3, 52 -- X-Exim-ID: 1cTZTr-000KOX-3Z 3, 52 -- X-Source: 3, 52 -- X-Source-Args: 3, 52 -- X-Source-Dir: 3, 52 -- X-Source-Sender: zsgenetics03.z.subnet.rcn.com (LarryPC) [146.115.5.114]:50951 3, 52 -- X-Source-Auth: les-at-zsgenetics.com 3, 52 -- X-Email-Count: 9 3, 52 -- X-Source-Cap: enNnYWRtMDE7enNnYWRtMDE7Z2F0b3IyMDEzLmhvc3RnYXRvci5jb20= ==============================End of - Headers==============================
I am considering purchasing a wall-mounted fume extractor for my microwave tissue processor which I will use for EM and paraffin work (to save space in my fume hood).
I have found some filters that have been rated for use with PFA and GA, but I am waiting to hear from the manufacturer regarding their use for OSO4, acetone, resins, and PO (doubt that info is available). I'd like to assume that the filters ("special carbon-treated") effectively filter out all of these, but I need to be as certain as possible. I will have undergraduates preparing samples and hope to use the microwave in my TEM and SEM courses, so I am very concerned about student safety.
Do any of you have any experience using these systems? Are they safe? Should I stop being so worried and just buy it? :)
Thank you! -Blanca ----------------------------------------- Blanca Carbajal Gonzalez, M.S. Director of Microscopy Science Center 50 College St Mount Holyoke College Clapp Laboratory 123 Office: 413-538-3118 Cell: 559-905-7138 bicarbaj-at-mtholyoke.edu MHC Microscopy
Why don't you run HVAC flex pipe from your wall-mounted fume extractor and plug it into the exhaust system? Failing that - modify one frame in the nearest window and exhaust filtered air outside. Decent carpenter or HVAC tech should be able to make such setup. Be safe :)
Valery Ray www.linkedin.com/in/valeryray ============================== PBS&T, MEO Engineering Company 290 Broadway, Suite 298 Methuen, MA 01844, USA Phone: +1-978-305-0479 - leave a message Mobie: +1-978-305-0479 - leave a message E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 1/17/2017 2:54 PM, bicarbaj-at-mtholyoke.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello everyone, } } I am considering purchasing a wall-mounted fume extractor for my } microwave tissue processor which I will use for EM and paraffin work } (to save space in my fume hood). } } I have found some filters that have been rated for use with PFA and } GA, but I am waiting to hear from the manufacturer regarding their use } for OSO4, acetone, resins, and PO (doubt that info is available). I'd } like to assume that the filters ("special carbon-treated") effectively } filter out all of these, but I need to be as certain as possible. I } will have undergraduates preparing samples and hope to use the } microwave in my TEM and SEM courses, so I am very concerned about } student safety. } } Do any of you have any experience using these systems? Are they safe? } Should I stop being so worried and just buy it? :) } } Thank you! } -Blanca } ----------------------------------------- } Blanca Carbajal Gonzalez, M.S. } Director of Microscopy } Science Center } 50 College St } Mount Holyoke College } Clapp Laboratory 123 } Office: 413-538-3118 } Cell: 559-905-7138 } bicarbaj-at-mtholyoke.edu } MHC Microscopy } } ==============================Original Headers============================== } 5, 37 -- From bicarbaj-at-mtholyoke.edu Tue Jan 17 13:53:10 2017 } 5, 37 -- Received: from mail-wm0-f41.google.com (mail-wm0-f41.google.com [74.125.82.41]) } 5, 37 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0HJr9bt008517 } 5, 37 -- for {microscopy-at-microscopy.com} ; Tue, 17 Jan 2017 13:53:10 -0600 } 5, 37 -- Received: by mail-wm0-f41.google.com with SMTP id c85so215510471wmi.1 } 5, 37 -- for {microscopy-at-microscopy.com} ; Tue, 17 Jan 2017 11:57:51 -0800 (PST) } 5, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 5, 37 -- d=mtholyoke-edu.20150623.gappssmtp.com; s=20150623; } 5, 37 -- h=mime-version:from:date:message-id:subject:to; } 5, 37 -- bh=i3zQfqAWrVLrQ5OUBetjF0orNpnlF8fG58FEU3N/gjQ=; } 5, 37 -- b=Ih3Zu1DKmSBwIjOXXfvaD8wUKZXMgQ/Skrs0fTiPrbLAfKUerCcDj+5TSipuaUEUqx } 5, 37 -- 6rWxgY+FIWULJTlWNwwnFQ+61mDDb576pKic9ky34Y7rush3Cy7xcAX7mDbO73czvICI } 5, 37 -- 8XJfquhBDMfUcWLVkUN0l26lz+Vcmy+GWNTN6gX0WsA8JtN+28afC98r7yqIERyOTXNi } 5, 37 -- 48JBpQ792PFU93GcGpaSayimqUw9d4waQrlsVczixHkDPDQY0TxTg/AUhROb/5MyQ0gN } 5, 37 -- +RyztwlnvLIs7ew+vcd8T/mcNp0BxICSby0xopSCHbczUtYJFjYhVGW/huiMC5rXggkx } 5, 37 -- LHWg== } 5, 37 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 5, 37 -- d=1e100.net; s=20161025; } 5, 37 -- h=x-gm-message-state:mime-version:from:date:message-id:subject:to; } 5, 37 -- bh=i3zQfqAWrVLrQ5OUBetjF0orNpnlF8fG58FEU3N/gjQ=; } 5, 37 -- b=U+if/Ewuf4u/L6Lf69/jPJQi+VP7M6dgS3AnWYc73In4vdpleVJMFKN8EQ9ibye14t } 5, 37 -- +eTfGWbyXpxUWJo26y36P+2e+bRaupqPbr3RiZAZ6MsW/s0PUJLzOsbPj8Tki8Vool5U } 5, 37 -- pQZZWFvS5u9qJv4+l7LPQr/YqktywVMpR3Gw08mTxu9CVZd5Zm9M8Rru53tsNbVuTMcD } 5, 37 -- kFrY9BbZ1tavtjN9JIX6jJZ04RvueA3HCT6Z6La9W71KPWuhv3LgcjtVLWXy33Ds/kZB } 5, 37 -- wERNwdwNtVDqNlufsR7LiW7QVxSTsOmzSTxRtjfE/Mft7uL1+v0V8x96rDGEkffvIFbQ } 5, 37 -- J+pg== } 5, 37 -- X-Gm-Message-State: AIkVDXJp+xHBYk0P2sZbfDj2eWFyJ5jIXAorGOhwBY8Ghqh+9Wi1U1OPT1bo/dn/X56HF7CQdZUcwARDFJBz4Z+O } 5, 37 -- X-Received: by 10.223.139.213 with SMTP id w21mr4712590wra.108.1484683070809; } 5, 37 -- Tue, 17 Jan 2017 11:57:50 -0800 (PST) } 5, 37 -- MIME-Version: 1.0 } 5, 37 -- Received: by 10.80.143.225 with HTTP; Tue, 17 Jan 2017 11:57:50 -0800 (PST) } 5, 37 -- From: Blanca Carbajal Gonzalez {bicarbaj-at-mtholyoke.edu} } 5, 37 -- Date: Tue, 17 Jan 2017 14:57:50 -0500 } 5, 37 -- Message-ID: {CAO94sumJEJEG0mw+2yoewmQSQRDodJE5CkOazdHvbhxzZB9vvg-at-mail.gmail.com} } 5, 37 -- Subject: EM: wall-mounted fume extractors } 5, 37 -- To: microscopy-at-microscopy.com } 5, 37 -- Content-Type: text/plain; charset=UTF-8 } ==============================End of - Headers============================== }
==============================Original Headers============================== 5, 35 -- From vray-at-partbeamsystech.com Tue Jan 17 15:06:27 2017 5, 35 -- Received: from nm7.bullet.mail.bf1.yahoo.com (nm7.bullet.mail.bf1.yahoo.com [98.139.212.166]) 5, 35 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0HL6RCd002096 5, 35 -- for {microscopy-at-microscopy.com} ; Tue, 17 Jan 2017 15:06:27 -0600 5, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1484687468; bh=l5cpaqW3TdyfzEAegATFvdK6g7ed2lq3S1IZPoJbhgc=; h=Subject:To:References:From:Cc:Date:In-Reply-To:From:Subject; b=IVa0gsZI7mBPw8PtaRUUdOtP1S0P1qEKvN2QjFLJRLXL3EJVOKlw0rstOfQ7T/SpQiqyoACLK5BUzGuHThzncXaeHJbbTyxX/YdqBZ4ZZRW3AAlD8B4fmptjqfViSGa8AV4hAiZup3nMkLdw0UBJ0df6zS1SE0tdVJ0ZH7So7ZX1qpiEfMo0GlFCArlX3yI7yrBdcSn518iLd6hWelE+ahU4oE7mvILSaOwygHC52F6O2KTFt5CuRHQEfDcThBvIXsrTKwRV+3ZEKTxKKMVQUOv9mKnJzE8b5OLgVwvjREkwU6IjHBCnsQoLkZyhEYw+LNnpn0cjKpqOrsnDAmnQNA== 5, 35 -- Received: from [98.139.215.143] by nm7.bullet.mail.bf1.yahoo.com with NNFMP; 17 Jan 2017 21:11:08 -0000 5, 35 -- Received: from [68.142.230.72] by tm14.bullet.mail.bf1.yahoo.com with NNFMP; 17 Jan 2017 21:11:08 -0000 5, 35 -- Received: from [127.0.0.1] by smtp229.mail.bf1.yahoo.com with NNFMP; 17 Jan 2017 21:11:08 -0000 5, 35 -- X-Yahoo-Newman-Id: 847699.48392.bm-at-smtp229.mail.bf1.yahoo.com 5, 35 -- X-Yahoo-Newman-Property: ymail-3 5, 35 -- X-YMail-OSG: YNOyXGEVM1lbZCj8QReoC7sPFpS.m7LNDCSzzp1zqIkfXyN 5, 35 -- PZfbaGJ.wrLJ6dxiP9vUeQQFqp2wp05lCT.SbNNWXA.IZClvNminro5.SgWv 5, 35 -- uQnZ39Lhi5781s8dBHIiBalFlcVWnhJ8296hAVCjczfjQtf_GMWq1oT4nsj5 5, 35 -- IdHlIWlXlArpkRG5krr8tik8ou9iC3V6ANZ6M62NbtZG4OOFqAMGglM8tjWN 5, 35 -- vEfogsc_WHzKUq.DHoik55BDAtLBpimLPHwlDybBQYEITpp4uo.mHr0vqW5w 5, 35 -- obkyV71bshLRhqQt.eX3Jedeh0FuB1Sg9OER4PnUyPpTy3cfGBibXCATHz8k 5, 35 -- a65Svg0.swVj8gfPBMzEUan4OtznTQJg0tt8qjqtRnLInYrdCllGayoaBwzz 5, 35 -- lhXbbMv1macilkjzFdeyEnyWA2bWlfzk6jjm.3vA1rY7wGauDOzpPGQJ0pI4 5, 35 -- m5cI1cHs4NjhTwlaCh9UFVF01spMVNK5_1hKKBtRnVyEroZHBr1wR358ywWF 5, 35 -- U1xbcdP6iKwQeAidkvS9ABwoqo6uVetgAoQcGyybMbDcrLXsxcBD1Ou2g.Ql 5, 35 -- R_ioR0MSA 5, 35 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 5, 35 -- Subject: Re: [Microscopy] EM: wall-mounted fume extractors 5, 35 -- To: bicarbaj-at-mtholyoke.edu 5, 35 -- References: {201701171954.v0HJsT6C009676-at-microscopy.com} 5, 35 -- From: Valery Ray {vray-at-partbeamsystech.com} 5, 35 -- Cc: microscopy-at-microscopy.com 5, 35 -- Message-ID: {7f10c025-790f-3b31-b7c4-ed6b6df19eff-at-partbeamsystech.com} 5, 35 -- Date: Tue, 17 Jan 2017 16:09:05 -0500 5, 35 -- User-Agent: Mozilla/5.0 (Windows NT 10.0; WOW64; rv:45.0) Gecko/20100101 5, 35 -- Thunderbird/45.6.0 5, 35 -- MIME-Version: 1.0 5, 35 -- In-Reply-To: {201701171954.v0HJsT6C009676-at-microscopy.com} 5, 35 -- Content-Type: text/plain; charset=windows-1252; format=flowed 5, 35 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: mreedman-at-rms.org.uk Name: Mel Reedman
Organization: Royal Microscopical Society
Title-Subject: [Filtered] MSM XX Abstract Submission Extended - Microscopy of Semi Conducting Materials
Message: Abstract deadline extended to Sunday 22 January and Registration now open! Microscopy of Semiconducting Materials XX (MSM XX) 9-13 April 2017 Lady Margaret Hall, University of Oxford
www.rms.org.uk/msm-xx
Scientific Organiser: Thomas Walther The conference will focus on the most recent advances in the study of the structural and electronic properties of semiconducting materials by the application of transmission and scanning electron microscopy. The latest developments in the use of other important micro-characterisation techniques including scanning probe microscopy, X-ray topography and diffraction will also be featured. Developments in materials science and technology covering the complete range of elemental and compound semiconductors will be described. Submit your abstract before midnight on Sunday 22 January 2017. We are accepting abstracts for both submitted talks and poster presentations at MSM XX on the following topics: - Analytical TEM - CL - Lattice Defects - Poly and Nano Crystals - Thin Films - Strained Layers and QWs - Nanowires - SPM & APFIM - SEM & FIB - Advanced Devices
Registration Registration for this meeting is now open. To register your place, please visit www.rms.org.uk/msm-xx. The registration fees are as follows:
Standard rate 575 RMS/IoP Member rate 475 Student rate 325 RMS/IoP Student member rate 280
To book en-suite accommodation for 75 a night directly with Lady Margaret Hall, please visit http://conference.lmh.ox.ac.uk/accommodation/. Use the code MSM2017, to show availability and take advantage of the preferential room rate. Find out more, submit an abstract and register at www.rms.org.uk/msm-xx
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From ronasyre474-at-gmail.com Wed Jan 18 09:59:01 2017 Return-Path: {ronasyre474-at-gmail.com} Received: from gmail.com ([183.111.66.21]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v0IFwwPS018121 for {microscopylistserverarchive6-at-microscopy.com} ; Wed, 18 Jan 2017 09:59:00 -0600 Message-ID: {1C255798.5C7C2740-at-gmail.com}
X-from: erica-at-scripps.edu
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Title-Subject: [Filtered] Position available in San Diego, California
Message: Position available for an experienced staff scientist (or postdoc) to perform single particle cryoEM studies on proteins relevant for viral hemorrhagic fever pathogenesis and medical defense.
You will have primary responsibility for data collection and oversight of a dedicated 300 keV Titan Halo with a Falcon 2 direct detector and for training of incoming students to the lab. In particular, we are looking for someone with strong interest and expertise in instrumentation, good communication skills, and a collaborative spirit who could pursue their own projects and assist those of others.
Our lab studies the proteins of viruses like Ebola, Marburg and Lassa, using structural biology and cellular analysis to understand how the very few proteins encoded by these viruses achieve a tremendous array of functions through the virus life cycle. Although we are primarily a structural biology lab ourselves, we direct a five-continent collaboration of academic, industrial and government scientists and clinicians. Hence, there is the opportunity here to see how the structures fit into the cellular, organismal and population contexts and to use structural biology as a roadmap to devlop much-needed vaccines and therapeutics.
Applications should be sent via email to erica-at-scripps.edu and should include a c.v., list of publications, names of references and a letter of interest. If you have any questions, just email me!
Erica Ollmann Saphire, Ph.D. Professor, Immunology & Microbial Science Co-Director, Center of Excellence, The Global Virus Network Director, Viral Hemorrhagic Fever Immunotherapeutic Consortium
The Scripps Research Institute 10550 North Torrey Pines Road, IMM-21 La Jolla, CA 92037 Tel: (858)784-8602 erica-at-scripps.edu
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Email: jhyun-at-gatan.com Name: John Hyun
Organization: Gatan
Title-Subject: [Filtered] TEM Application Scientist Opening
Message: Location: Pleasanton, CA (remote locations considered)
Position Summary: This position is responsible for supporting the sales and marketing efforts of Gatans highly successful Analytical TEM product line (GIF and EELS systems). The position is based at Gatans Pleasanton, CA headquarters but will support analytical products worldwide.
Job Responsibilities: Provide pre-sales applications support with presentations and demonstrations of Gatan products on a range of TEM models Provide post-sales customer support and on-site customer training Provide on-site advanced customer training and telephone/email support Provide general applications support, telephone/email support, and limited hardware and software troubleshooting Propose design enhancements and improvements to Gatan hardware and software Work with Gatan R&D in the development and testing of new products and applications Represent Gatan at scientific conferences
Education/Experience: Advanced degree in science or engineering (or equivalent experience) is required Strong background in transmission electron microscopy, specifically in relation to the acquisition, application, interpretation, and discussion of analytical TEM techniques including EELS, EDS, EFTEM, and STEM techniques Hands-on, post-graduate level experience in advanced analytical TEM applications with a proven publication record Qualifications: Ability to interface effectively with customers at all experience levels while projecting a strong client service attitude Excellent verbal, written, and interpersonal communication skills in English are essential Ability to work on complex learning and development problems as well as teach highly technical information is essential Significant domestic and international travel required- 50% or higher at times.
To Apply: Interested candidates should submit a resume to hrus-at-gatan.com. Gatan is an equal opportunity employer. All qualified applicants will receive consideration for employment without regard to race, religion, color, national origin, sex, sexual orientation, gender identity, age, status as a protected veteran, among other things, or status as a qualified individual with disability.
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Email: heckman-at-bgsu.edu Name: Carol Heckman
Organization: Bowling Green State University
Title-Subject: [Filtered] Gatan ChromaCL
Message: We bought a GATAN cathodoluminescence detector some years ago, and had it installed on our Hitachi 2700S SEM. It breaks vacuum sometimes when it is entered into the column. We need cathodoluminescence mode for several projects.
Does anybody have the same problem? If so, what did you do and did you solve it?
Carol Heckman
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Since the problem is intermittent and occurs during insertion, chances are you have either crack in vacuum bellows, or warn-out O-ring somewhere in feedthrough. You would probably need to call in either OEM or third-party service, or find someone local who can bring in a leak detector and troubleshoot this on site.
Best Wishes :)
Valery Ray www.linkedin.com/in/valeryray ============================== PBS&T, MEO Engineering Company 290 Broadway, Suite 298 Methuen, MA 01844, USA Phone: +1-978-305-0479 - leave a message Mobie: +1-978-305-0479 - leave a message E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 1/21/2017 3:53 PM, microscopy.listserver-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both heckman-at-bgsu.edu as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: heckman-at-bgsu.edu } Name: Carol Heckman } } Organization: Bowling Green State University } } Title-Subject: [Filtered] Gatan ChromaCL } } Message: We bought a GATAN cathodoluminescence detector some years ago, and had it installed on our } Hitachi 2700S SEM. It breaks vacuum sometimes when it is entered into the column. We need } cathodoluminescence mode for several projects. } } Does anybody have the same problem? If so, what did you do and did you solve it? } } } Carol Heckman } } } Login Host: 129.1.85.73 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } }
==============================Original Headers============================== 5, 35 -- From vray-at-partbeamsystech.com Sat Jan 21 16:57:54 2017 5, 35 -- Received: from nm17-vm1.bullet.mail.bf1.yahoo.com (nm17-vm1.bullet.mail.bf1.yahoo.com [98.139.213.55]) 5, 35 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0LMvr5W004903 5, 35 -- for {microscopy-at-microscopy.com} ; Sat, 21 Jan 2017 16:57:54 -0600 5, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1485039770; bh=190jyd0D/6uDYtpxJWYjPSZROobRZ5hN53vjca3ckd4=; h=Subject:To:References:Cc:From:Date:In-Reply-To:From:Subject; b=J4AAeJARsZgWxhgsCMqbnHTUFZpS27ftaQIoqGwU5n0Qg4XljClwS68NqMJZ40b9i83m3bKWCCEUgMSIPL4otei91LEXPTUCmJeh0t2ZipI7jUe3gTcsO/tdBT4VhSP/za6D8diPtWoq33nmmSxX/sr2g5WSt2F9gDtdtUwuPaXq/2mNVNDVDVPTdCpdp+eRoV4bztzRS2dXYvbqnhk/9R0m/EXupXjWW3zI+zyiNCIPVSBPfLt1yPuFQslbEcsipc3+1UQCILwqU10oGSFm0gBlQ6J+TCtVolOpXY2nq05GEsRjneTeCC5aGCTxiNTeVaHY1aRXAP64VNd/hl5liQ== 5, 35 -- Received: from [98.139.215.142] by nm17.bullet.mail.bf1.yahoo.com with NNFMP; 21 Jan 2017 23:02:50 -0000 5, 35 -- Received: from [98.139.211.203] by tm13.bullet.mail.bf1.yahoo.com with NNFMP; 21 Jan 2017 23:02:50 -0000 5, 35 -- Received: from [127.0.0.1] by smtp212.mail.bf1.yahoo.com with NNFMP; 21 Jan 2017 23:02:50 -0000 5, 35 -- X-Yahoo-Newman-Id: 271930.99062.bm-at-smtp212.mail.bf1.yahoo.com 5, 35 -- X-Yahoo-Newman-Property: ymail-3 5, 35 -- X-YMail-OSG: gjHUqHkVM1k8KApY7QGPOWJUzZ.RBYwDDsu73Xm.0hcMFgY 5, 35 -- XheruY2ddVZ8sRXDt3qe6VukrUSQRnGWM3579WAy9Me0uNKe5OVl_1OggmgW 5, 35 -- yb4I9UoVJBeuZT4se7sFoukN08xHeBkhL8XXMb7d.tMf2Heqd.KqV9ULrRjI 5, 35 -- mBWGoEfICnw6Vavtp_U4hYOrRW0o6spIGHOVccdytFmi73PAnVGcst1nes.v 5, 35 -- o9wdXHQspbFq_.36oSbNkKMIJpG6uMpC4hBCFyUF6pyJXXt83EODLk8DFAUg 5, 35 -- jsNUVNdq8lGgQxIH.jykaLZIaQm7pwjCfU7LM33RNVrACnf6jXadDroCaHq2 5, 35 -- 9waWCa5cPn2oZdFFyma.Y7tXEUoOnX7tLsxbx5tNAQSktMikrCFstVayoYy1 5, 35 -- 1TQ.1w30Tv_kJVAHclW2Bu1WkqkvHg6LVwXzPfu86ndGEwol8u9joIM.rkha 5, 35 -- BPnDm1eMSwEBhcv5u4ZT5l5FrOQdocAnBPr5O1fV5EYgsIFYCx9T9zkSuO3n 5, 35 -- DbruOdBrD9bvzs4a85man.08wC0a39.Jt1z0qUzioBgOq7DCdjmd3usZRr3i 5, 35 -- M45HlTJc4 5, 35 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 5, 35 -- Subject: Re: [Microscopy] viaWWW: Gatan ChromaCL Help Needed 5, 35 -- To: heckman-at-bgsu.edu 5, 35 -- References: {201701212053.v0LKrG0Q012609-at-microscopy.com} 5, 35 -- Cc: microscopy-at-microscopy.com 5, 35 -- From: Valery Ray {vray-at-partbeamsystech.com} 5, 35 -- Message-ID: {69cb53e9-760a-5666-5610-062fd2d21155-at-partbeamsystech.com} 5, 35 -- Date: Sat, 21 Jan 2017 18:00:38 -0500 5, 35 -- User-Agent: Mozilla/5.0 (Windows NT 10.0; WOW64; rv:45.0) Gecko/20100101 5, 35 -- Thunderbird/45.6.0 5, 35 -- MIME-Version: 1.0 5, 35 -- In-Reply-To: {201701212053.v0LKrG0Q012609-at-microscopy.com} 5, 35 -- Content-Type: text/plain; charset=windows-1252; format=flowed 5, 35 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
From sawijama5-at-gmail.com Sat Jan 21 19:21:49 2017 Return-Path: {sawijama5-at-gmail.com} Received: from gmail.com ([210.92.18.86]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v0M1LkoN032594 for {microscopylistserverarchive6-at-microscopy.com} ; Sat, 21 Jan 2017 19:21:48 -0600 Message-ID: {18CCB40A.A1DE92E8-at-gmail.com}
All, For those interested in the microanalysis side of things, I have available to donate a Physical Electronics Quantum 2000 Scanning ESCA Microprobe. The Quantum is the predecessor to the PHI Quantera model.
This X-ray Photoelectron Spectroscopy/Electron Spectroscopy for Chemical Analysis system has been sitting powered down but connected to clean nitrogen for a little over a year, after developing a problem in a small power supply. That power supply has been priced at about $300. Prior to that, it had a noise issue, so I believe there is a problem with the detector or in the detector electronics that would need to be addressed. The unit was purchased in 1999 for approximately $600,000, and used for several years with very clean samples. In 2006 it was upgraded to a Windows XP data system.
My company is relocating R&D facilities from Duncan, SC (the current instrument location) and no longer has pressing need for the technique. Rather than relocate the instrument, we would like to donate it to a university or other non-profit. Recipient would be responsible for transportation and related costs. As part of an effort to give back to our local community, preference would be given to schools in closer proximity to our new headquarters in Charlotte, NC, but all interested parties are encouraged to inquire by emailing me at james.passmore+xps-at-sealedair.com.
Regards, Jim
-- Jim Passmore Principal Scientist, R&D
Sealed Air Corporation P: 864.433.2927 100 Rogers Bridge Rd., Bldg A Duncan, SC 29334
==============================Original Headers============================== 6, 34 -- From james.passmore-at-sealedair.com Mon Jan 23 08:10:43 2017 6, 34 -- Received: from mail-wm0-f48.google.com (mail-wm0-f48.google.com [74.125.82.48]) 6, 34 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0NEAg9I016510 6, 34 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Jan 2017 08:10:42 -0600 6, 34 -- Received: by mail-wm0-f48.google.com with SMTP id c85so134635118wmi.1 6, 34 -- for {Microscopy-at-microscopy.com} ; Mon, 23 Jan 2017 06:15:45 -0800 (PST) 6, 34 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 34 -- d=sealedair.com; s=google; 6, 34 -- h=mime-version:from:date:message-id:subject:to; 6, 34 -- bh=6VLh4FtX3pQY8h/IfK0aO6Hi3FYP7s+9gDIC1IuQkFc=; 6, 34 -- b=bV1xRioQ4lu3ejK9LWwtia2P1OKG1kdfmusDLvrL3cSkGqheV7RcVh9XoADmTFSWPK 6, 34 -- K1LPOhu40KfscbS+LGIwFIxBYDT0HGFxSis3onRahs9zxME2Yu6+gYejsV/VtCLISrE5 6, 34 -- bDNWG2JR7LNgKLkiSDbKT/OGxF7Q9IOCO/OYM= 6, 34 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 34 -- d=1e100.net; s=20161025; 6, 34 -- h=x-gm-message-state:mime-version:from:date:message-id:subject:to; 6, 34 -- bh=6VLh4FtX3pQY8h/IfK0aO6Hi3FYP7s+9gDIC1IuQkFc=; 6, 34 -- b=Q2LuygXFF8CYahuQdGfUbxDR61fcnXr1ti0+MNynWAfuR4jorZBOWOu4gVej/OTqPp 6, 34 -- PLd5JYfTz+SpvaJOKyAu/YpYS+R4M9U0zoAN5cPw666iNoiEBrB2Mbb5jUse+5jXrqoZ 6, 34 -- 5LrvSmWY9vJ/bgKxve2jgYNbai2XXGlULLmyWQxqAt/D+Hnyq6KIHcCnNEhMdy3nF/i8 6, 34 -- Sgq5v7FmoUXTRhWOSMk0rAEJalt6TwtpsbAZw//xvUhb6xvtcVl7rjTwl+YbWOMIx8V1 6, 34 -- PCVv4SyaO69pcOsCoLQhIFBV1uFlRelEcUBsXvtEqeLc82hbdE5yQ4c1Nr2LcfpEgkSD 6, 34 -- xBQA== 6, 34 -- X-Gm-Message-State: AIkVDXLcejNUpUufk0JEvmMi+q+jA59jQqOw584Eyl4JRBTUsVszJUkpKmb6slBb0RBz/SGgBFOyBsfhpXoRv7zG 6, 34 -- X-Received: by 10.223.136.155 with SMTP id f27mr23112431wrf.98.1485180944288; 6, 34 -- Mon, 23 Jan 2017 06:15:44 -0800 (PST) 6, 34 -- MIME-Version: 1.0 6, 34 -- Received: by 10.194.239.234 with HTTP; Mon, 23 Jan 2017 06:15:13 -0800 (PST) 6, 34 -- From: Jim Passmore {james.passmore-at-sealedair.com} 6, 34 -- Date: Mon, 23 Jan 2017 09:15:13 -0500 6, 34 -- Message-ID: {CAGV-iYZ78u+nQiejE1a46KNBRXz=8cW7pMToEGDphbW-L7V0-g-at-mail.gmail.com} 6, 34 -- Subject: XPS/ESCA system for donation 6, 34 -- To: microscopy {Microscopy-at-microscopy.com} 6, 34 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
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Email: cni-at-udel.edu Name: Chaoying Ni
Organization: University of Delaware
Title-Subject: [Filtered] Research Associate I (28N) - Electron Microscopy
Message: Research Associate I - (Grade 28N) Materials Science and Engineering
Deadline: January 26, 2017
CONTEXT OF THE JOB:
Under limited supervision, performs a variety of complex technical laboratory duties including the design and construction of test equipment. Supervises and trains personnel. The principal emphasis is on working independently and exercising personal initiative in conducting experiments using complex electron and light microscopes, scanning probe microscopes and associated sample preparation equipment and experiment devices.
QUALIFICATIONS:
Requires a minimum of a Bachelor's degree and one year of experience in laboratory work in a relevant field as well as experience with the necessary physical, electronic and chemical systems. Supervisory experience preferred. Related progressive experience beyond a high school diploma or GED may be substituted for required education or additional related education may be substituted for required experience. Requires knowledge of laboratory techniques, procedures and instrumentation.
Requires abilities to read and interpret complex operations manuals, drawings and design, interpret problems and design equipment for the particular tasks, assume responsibility and take initiative to perform tasks, work independently, train and instruct others, and communicate effectively and interact well with people of all ages and diverse backgrounds.
OCCUPATIONAL EXPOSURES:
May be required to use personal protective equipment to prevent exposure to hazardous materials.
More details: https://udjobs.nss.udel.edu:4450/psc/RESUME/EMPLOYEE/HRMS/s/WEBLIB_UDEL.ISCRIPT1.FieldFormula.IScript_Careers?Page=HRS_CE_HM_PRE&Action=A&SiteId=888&
or
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Email: mikeh-at-ncimicro.com Name: Mike Hehr
Organization: NCI
Title-Subject: [Filtered] Nano Technology Sales Position
Message: We are currently seeking an individual with EM Sample Prep experience for a new technical sales position. This includes, Ion Milling, Ultra Microtomy, CLEM and High Pressure Freezing. This role will be to support customers in the vast Midwest. If interested, please submit you resume to mikeh-at-ncimicro.com
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Those of you actively working with FIB in either device or analytical applications could find the forwarded below message of interest:
--- On Tue, 1/24/17, Hugo Bender (imec) {Hugo.Bender-at-imec.be} wrote:
} From: Hugo Bender (imec) {Hugo.Bender-at-imec.be} } Subject: new link to EFUG website : http://efug.imec.be/ } To: "Hugo Bender (imec)" {Hugo.Bender-at-imec.be} } Date: Tuesday, January 24, 2017, 1:18 AM } } Dear EFUG’ers, } FIB’ers, } } } Please note that the link to the } EFUG website is changed to : http://efug.imec.be/ } } The old link will soon not be } operational anymore. So update your bookmarks } now! } } } There will be again 2 FIB meetings } in Europe in 2017: } } 1st EUFN } workshop } (Former DACH } Workshop) : general FIB and FIB } applications } 4-5 July 2017, Graz, Austria. } } Abstract : deadline 30 April 2017 } http://www.eu-f-n.org/ } } } 21st } EFUG meeting } during ESREF : } semiconductor and device applications of } FIB } Week 25-29 September 2017, } Bordeaux France } http://efug.imec.be/ } } } Please forward to your FIB } colleagues ! } } } Best regards } } Hugo } } } Hugo } Bender } Principal Member } Technical Staff } T +32 16 28 } 1304 } hugo.bender-at-imec.be } I } www.imec.be } Kapeldreef 75 } } I 3001 } Leuven } I } Belgium } } imec e-mail } disclaimer } } } } } }
==============================Original Headers============================== 5, 32 -- From vray-at-partbeamsystech.com Tue Jan 24 15:01:19 2017 5, 32 -- Received: from nm36-vm9.bullet.mail.gq1.yahoo.com (nm36-vm9.bullet.mail.gq1.yahoo.com [98.136.216.170]) 5, 32 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0OL1Jv9003076 5, 32 -- for {microscopy-at-microscopy.com} ; Tue, 24 Jan 2017 15:01:19 -0600 5, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1485291986; bh=dFUfiK1R0Nwm1ASBGNFmLH70A5tNNwLm2p8kH/0EcFQ=; h=Date:From:Reply-To:To:Subject:References:From:Subject; b=Im9wVUndp0XW1PhTD0+X7vFPshTWlSH74EbOHWLANUuSM6Wg/kFVbfIVhVEFw1KHZRRjBZ2s2N6CihsqyTOlm5+Azw6rohBJMPGOqBUu0zAVLc8+rBca9j+HlGOxTrOZwYAaomrij9Kn/KsfG734i2Xr+5IBtW6gmS+O3Mm6bbC4I/omkN6E8qgRtH9tOsPttvA2FegxppTf4hIRvhinpj0y5YrQxRjgJPdhVZomNn6JzZcGIwt53Y8Ed+tfdGaZXG/bK2AJHbvbqqqkdJ6FoXw0HwsAaOuypzIt6bdXhgi2obHSaIJyjXzBo9zwYh1xcGQG1BoODncXRNb//t9Mxg== 5, 32 -- Received: from [127.0.0.1] by nm36.bullet.mail.gq1.yahoo.com with NNFMP; 24 Jan 2017 21:06:26 -0000 5, 32 -- Received: from [216.39.60.183] by nm36.bullet.mail.gq1.yahoo.com with NNFMP; 24 Jan 2017 21:03:13 -0000 5, 32 -- Received: from [98.139.170.180] by tm19.bullet.mail.gq1.yahoo.com with NNFMP; 24 Jan 2017 21:03:13 -0000 5, 32 -- Received: from [98.139.215.229] by tm23.bullet.mail.bf1.yahoo.com with NNFMP; 24 Jan 2017 21:03:13 -0000 5, 32 -- Received: from [127.0.0.1] by omp1069.mail.bf1.yahoo.com with NNFMP; 24 Jan 2017 21:03:13 -0000 5, 32 -- X-Yahoo-Newman-Property: ymail-4 5, 32 -- X-Yahoo-Newman-Id: 420308.34066.bm-at-omp1069.mail.bf1.yahoo.com 5, 32 -- X-YMail-OSG: wLtB7pUVM1kVpu5oGhSOQ05wi1KTgP762ggHuisxFiTI.sSES33ux5T8Cf3yeP2 5, 32 -- OP67hpheJqYAXzxYIvNEOXW27zfVt.RbuM8C7Ctxuo3dZbNXOcfQkcmtjfCjRmrxq6ASu3tjR8mZ 5, 32 -- RUcqoW4XWViD6w.TNsNcUuyqhKkFkAe7D41EVMaI9rxK1bk861zvkJ2IiK6CKe4cQCavxS7WYUQC 5, 32 -- 8ceR7jvD37v7n6Osc6Y4sAJrduKH1e.IgUiSRwHgkkIzdoAFeIBpFCPaNCM8kCrHPxEHQinX1o.Q 5, 32 -- rDJYROriwwLTS5b_ib0uxM0oSrPb0vWPRXe1z9DXh01_dwLTxrQSuUbgMLD1qcX1N.3VIR86E6fZ 5, 32 -- 4yKhmse1lCjcaDc8vTTO6djp6fOceRFiJDeeSXLSb.ELwpdBbM7bX7QU08JXk_WJ6ipauk886ej6 5, 32 -- _10.DQqwf.llmH2uMEU91ps9bGpUgPHVFUjZMBpKdSXuSF1IO2g2gUcephHmT3KNuMDuK3sDU9Uw 5, 32 -- 2oWPAJNWRigRUJubS2RtRtGJ5m2Tlh80YcFLybA7qFHOkte8FtF9Hv0PPsUsMIOlIrA-- 5, 32 -- Received: from jws400105.mail.bf2.yahoo.com by sendmailws106.mail.bf1.yahoo.com; Tue, 24 Jan 2017 21:03:13 +0000; 1485291793.041 5, 32 -- Date: Tue, 24 Jan 2017 21:03:12 +0000 (UTC) 5, 32 -- From: {vray-at-partbeamsystech.com} 5, 32 -- Reply-To: {vray-at-partbeamsystech.com} 5, 32 -- To: {microscopy-at-microscopy.com} 5, 32 -- Message-ID: {1986517122.3093933.1485291792844-at-mail.yahoo.com} 5, 32 -- Subject: Fw: New link to EFUG website and EFUG/EUFN meeting announcements 5, 32 -- MIME-Version: 1.0 5, 32 -- Content-Type: text/plain; charset=UTF-8 5, 32 -- References: {1986517122.3093933.1485291792844.ref-at-mail.yahoo.com} 5, 32 -- Content-Transfer-Encoding: 8bit 5, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v0OL1Jv9003076 ==============================End of - Headers==============================
From michcons458-at-gmail.com Tue Jan 24 16:35:33 2017 Return-Path: {michcons458-at-gmail.com} Received: from gmail.com ([210.92.18.86]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v0OMZUvo027260 for {microscopylistserverarchive6-at-microscopy.com} ; Tue, 24 Jan 2017 16:35:32 -0600 Message-ID: {4149520D.C242A3C7-at-gmail.com}
I am a relatively inexperienced TEM tech and am having problems with my PTA. Each time I make fresh PTA, I end up with an image full of stain blobs. Obviously this is useless. I have tried purchasing fresh PTA, but that didn't make any difference. I have also used BSA, which helps some, but not a lot. Help! Any ideas are welcome.
Jami
Jami Carroll Lab Tech II - Virology Breathitt Veterinary Center Hopkinsville, KY 270-886-3959 jcarroll-at-murraystate.edu
==============================Original Headers============================== 3, 41 -- From jcarroll-at-murraystate.edu Wed Jan 25 14:29:39 2017 3, 41 -- Received: from isenguard1.murraystate.edu (isenguard1.murraystate.edu [216.249.159.66]) 3, 41 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0PKTbsW003097 3, 41 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Jan 2017 14:29:38 -0600 3, 41 -- Received: (qmail 9596 invoked by uid 514); 25 Jan 2017 14:36:00 -0600 3, 41 -- Received: from mail-io0-f200.google.com (209.85.223.200) 3, 41 -- by isenguard1.murraystate.edu with AES128-SHA encrypted SMTP; 25 Jan 2017 14:36:00 -0600 3, 41 -- Received: by mail-io0-f200.google.com with SMTP id j13so22886139iod.6 3, 41 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Jan 2017 12:34:48 -0800 (PST) 3, 41 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 41 -- d=murraystate.edu; s=google; 3, 41 -- h=from:mime-version:thread-index:date:message-id:subject:to; 3, 41 -- bh=TbOf5vsspNPFILyzcNL/dvwC+HvU+9H0+31YCGmgl/E=; 3, 41 -- b=WZ6VhdSIqrdc5oOkZa7X1Rmj+5334BWEDO33FudPIC3aJovIJXOWOrdWfyhmfHMaUJ 3, 41 -- 3lyo64W+gSTWA9EkIT/48KPnSDGAbk8beOzVQcqGvxuV5ygp/Q1N6BEuM5EHhIrlpQSb 3, 41 -- Ls9EbdTtw7y4Kjg32hUlIzYyg9SCW9KYjMLsc= 3, 41 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 41 -- d=1e100.net; s=20161025; 3, 41 -- h=x-gm-message-state:from:mime-version:thread-index:date:message-id 3, 41 -- :subject:to; 3, 41 -- bh=TbOf5vsspNPFILyzcNL/dvwC+HvU+9H0+31YCGmgl/E=; 3, 41 -- b=Jn648cp78H0CzTgjnBLa8maTkzAHTTnAjlUfEZ/A0n8eozRDZ1HZRy2ReC6H6SPo56 3, 41 -- bprdzy1+8O1V1+5s6Fi3E13J0Ad4Hrgfm5OviMFW3WOqsc3bBvxpWgTf4DGwsVfDnha+ 3, 41 -- QgpdEVd3DTrzyhlnkoYN0PjkunxisCYDWKWSyMEg2Wx1W613he2omkJzb2+PzIAJPdCJ 3, 41 -- +Xcmtjb26k25Y2bYIF/KvVqah+R1NAqLabeL5mHeoUhTzzRz96TTTutM1UWcU298LDCn 3, 41 -- 6qBeB5JCKN3XtvNe7W+qQq0b3+5GWoPEK0MUR610ZX8KhzPMlowdnpl+zMM+058oyUhf 3, 41 -- oVQQ== 3, 41 -- X-Gm-Message-State: AIkVDXJEKzPrp/T8tSiVuZJre5xfryNuP0448Sa+UFXWZ4MC9fvb9dc6VeOi4qI+6KSXlDOH48VQv+wBIH8OkDdlGeluC39/fcXLBOUu0V7jOSAG6EmBQfFpOu2Ju50ZUGfZAWmRG2h7rjRFAz3WXU7rbu6r5YzvzAI= 3, 41 -- X-Received: by 10.107.10.24 with SMTP id u24mr34419759ioi.94.1485376487879; 3, 41 -- Wed, 25 Jan 2017 12:34:47 -0800 (PST) 3, 41 -- X-Received: by 10.107.10.24 with SMTP id u24mr34419752ioi.94.1485376487758; 3, 41 -- Wed, 25 Jan 2017 12:34:47 -0800 (PST) 3, 41 -- From: Jami Carroll {jcarroll-at-murraystate.edu} 3, 41 -- MIME-Version: 1.0 3, 41 -- X-Mailer: Microsoft Outlook 15.0 3, 41 -- Thread-Index: AdJ3SlzXhP0ZYrc6S9+CcE8EBSgGPw== 3, 41 -- Date: Wed, 25 Jan 2017 14:34:46 -0600 3, 41 -- Message-ID: {316b19cf1154b769f967a2c0a91d4a27-at-mail.gmail.com} 3, 41 -- Subject: TEM negative staining issues 3, 41 -- To: Microscopy-at-microscopy.com 3, 41 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
I do hope that you have consulted Google with: "PTA negative staining" to try to find some troubleshooting advice from experts. What I am about to offer is all true and necessary for me. Thus, necessary for everyone else - if I don't think about your problem.
I can state with absolutely strictly personal experience, that one - Me! - throws time away all the time if the water one - Me, again - starts with has not been deionized, glass distilled, filtered with the 22um ['micro' or 'Meuw' meter] 57mm Millipore filter, and, if to be used in MolBio, laced with RNAse Inhibitor [https://en.wikipedia.org/wiki/Ribonuclease_inhibitor ]. Once this 'Monkish' procedure has been completed with both song and dance, I can be assured of a good experiment.
If the 'blobs' are larger than .22um, then I recommend that you run your PTA thru a 0.22um 12mm Millipore filter using a small Swinney holder with a 1ml disposable syringe. You could also try a dose of high frequency sonic jewelry cleaner borrowed from your Mom or your 'gal.'
I have given you both mystic and sound recommendations to attack your problem. These are things I have tried in the past, but, I admit, nowhere near Kentucky. [In the old days, histologists who Wintered in Maine and Summered in Florida, had two types of paraffin: one for Maine; and a second for Florida - each with a 'Climatized' paraffin recipe!
I do humarize when the subject permits, so, please understand that I am old (77), sometime called "Grumpy39," and cannot restrain myself any more.
Cheers, and good luck,
Fred Monson
P.S. If you come to a moment where you simply have to change brains, sit down and answer this simple question: "How many digital cameras do you have with you right now? After you answer, please take a few minutes to determine why some old guy would ask such a silly question to take my mind of my problem with PTA. There you go. My answer is that 98% of educated people give the wrong answer. You should know that I only gave ONE multiple answer test in the 12 years that I instructed in biology in development and electron microscopy. In histology, I felt obligated to remind students that all they would learn in histology would be about dead stuff. Cheers, again.
P.S. With that cheerful business done, please remember that life/death of virions is still under misunderstanding. Be careful - always. P.S. In the creek, doped with E. Coli, for each of them there are 100 phage. P.S. Oh! Yes! If you have a need of concentrating the sample, consider a Beckman Airfuge with a TEM head.
Frederick C. Monson, PhD Technical Director Center for Microanalysis and Imaging, Research and Training (CMIRT) West Chester University of PA Geology-Astronomy 750 South Church St. West Chester, PA, 19383 fmonson-at-wcupa.edu 610-738-0437(Work) Fc.monson-at-gmail.com for more help if you need it: i.e., refereces, and other stuff.
-----Original Message----- X-from: jcarroll-at-murraystate.edu [mailto:jcarroll-at-murraystate.edu] Sent: Wednesday, January 25, 2017 3:41 PM To: Monson, Frederick {FMonson-at-wcupa.edu}
I am a relatively inexperienced TEM tech and am having problems with my PTA. Each time I make fresh PTA, I end up with an image full of stain blobs. Obviously this is useless. I have tried purchasing fresh PTA, but that didn't make any difference. I have also used BSA, which helps some, but not a lot. Help! Any ideas are welcome.
Jami
Jami Carroll Lab Tech II - Virology Breathitt Veterinary Center Hopkinsville, KY 270-886-3959 jcarroll-at-murraystate.edu
==============================Original Headers============================== 3, 41 -- From jcarroll-at-murraystate.edu Wed Jan 25 14:29:39 2017 3, 41 -- Received: from isenguard1.murraystate.edu (isenguard1.murraystate.edu [216.249.159.66]) 3, 41 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0PKTbsW003097 3, 41 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Jan 2017 14:29:38 -0600 3, 41 -- Received: (qmail 9596 invoked by uid 514); 25 Jan 2017 14:36:00 -0600 3, 41 -- Received: from mail-io0-f200.google.com (209.85.223.200) 3, 41 -- by isenguard1.murraystate.edu with AES128-SHA encrypted SMTP; 25 Jan 2017 14:36:00 -0600 3, 41 -- Received: by mail-io0-f200.google.com with SMTP id j13so22886139iod.6 3, 41 -- for {Microscopy-at-microscopy.com} ; Wed, 25 Jan 2017 12:34:48 -0800 (PST) 3, 41 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 41 -- d=murraystate.edu; s=google; 3, 41 -- h=from:mime-version:thread-index:date:message-id:subject:to; 3, 41 -- bh=TbOf5vsspNPFILyzcNL/dvwC+HvU+9H0+31YCGmgl/E=; 3, 41 -- b=WZ6VhdSIqrdc5oOkZa7X1Rmj+5334BWEDO33FudPIC3aJovIJXOWOrdWfyhmfHMaUJ 3, 41 -- 3lyo64W+gSTWA9EkIT/48KPnSDGAbk8beOzVQcqGvxuV5ygp/Q1N6BEuM5EHhIrlpQSb 3, 41 -- Ls9EbdTtw7y4Kjg32hUlIzYyg9SCW9KYjMLsc= 3, 41 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 41 -- d=1e100.net; s=20161025; 3, 41 -- h=x-gm-message-state:from:mime-version:thread-index:date:message-id 3, 41 -- :subject:to; 3, 41 -- bh=TbOf5vsspNPFILyzcNL/dvwC+HvU+9H0+31YCGmgl/E=; 3, 41 -- b=Jn648cp78H0CzTgjnBLa8maTkzAHTTnAjlUfEZ/A0n8eozRDZ1HZRy2ReC6H6SPo56 3, 41 -- bprdzy1+8O1V1+5s6Fi3E13J0Ad4Hrgfm5OviMFW3WOqsc3bBvxpWgTf4DGwsVfDnha+ 3, 41 -- QgpdEVd3DTrzyhlnkoYN0PjkunxisCYDWKWSyMEg2Wx1W613he2omkJzb2+PzIAJPdCJ 3, 41 -- +Xcmtjb26k25Y2bYIF/KvVqah+R1NAqLabeL5mHeoUhTzzRz96TTTutM1UWcU298LDCn 3, 41 -- 6qBeB5JCKN3XtvNe7W+qQq0b3+5GWoPEK0MUR610ZX8KhzPMlowdnpl+zMM+058oyUhf 3, 41 -- oVQQ== 3, 41 -- X-Gm-Message-State: AIkVDXJEKzPrp/T8tSiVuZJre5xfryNuP0448Sa+UFXWZ4MC9fvb9dc6VeOi4qI+6KSXlDOH48VQv+wBIH8OkDdlGeluC39/fcXLBOUu0V7jOSAG6EmBQfFpOu2Ju50ZUGfZAWmRG2h7rjRFAz3WXU7rbu6r5YzvzAI= 3, 41 -- X-Received: by 10.107.10.24 with SMTP id u24mr34419759ioi.94.1485376487879; 3, 41 -- Wed, 25 Jan 2017 12:34:47 -0800 (PST) 3, 41 -- X-Received: by 10.107.10.24 with SMTP id u24mr34419752ioi.94.1485376487758; 3, 41 -- Wed, 25 Jan 2017 12:34:47 -0800 (PST) 3, 41 -- From: Jami Carroll {jcarroll-at-murraystate.edu} 3, 41 -- MIME-Version: 1.0 3, 41 -- X-Mailer: Microsoft Outlook 15.0 3, 41 -- Thread-Index: AdJ3SlzXhP0ZYrc6S9+CcE8EBSgGPw== 3, 41 -- Date: Wed, 25 Jan 2017 14:34:46 -0600 3, 41 -- Message-ID: {316b19cf1154b769f967a2c0a91d4a27-at-mail.gmail.com} 3, 41 -- Subject: TEM negative staining issues 3, 41 -- To: Microscopy-at-microscopy.com 3, 41 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
==============================Original Headers============================== 24, 41 -- From FMonson-at-wcupa.edu Wed Jan 25 15:42:06 2017 24, 41 -- Received: from MX01.WCUPA.EDU (mx01.wcupa.edu [144.26.63.2]) 24, 41 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0PLg6JN026497 24, 41 -- for {microscopy-at-microscopy.com} ; Wed, 25 Jan 2017 15:42:06 -0600 24, 41 -- X-ASG-Debug-ID: 1485380837-08911c2a5835b0a0001-4CH8be 24, 41 -- Received: from WCUXCHP01.PASSHE.LCL ([144.26.2.137]) by MX01.WCUPA.EDU with ESMTP id qF5wgjG9ogHROS2a (version=TLSv1.2 cipher=ECDHE-RSA-AES256-SHA384 bits=256 verify=NO); Wed, 25 Jan 2017 16:47:17 -0500 (EST) 24, 41 -- X-Barracuda-Envelope-From: FMonson-at-wcupa.edu 24, 41 -- X-Barracuda-Apparent-Source-IP: 144.26.2.137 24, 41 -- X-ASG-Whitelist: Client 24, 41 -- Received: from WCUXCHP08.PASSHE.LCL (144.26.2.144) by WCUXCHP01.PASSHE.LCL 24, 41 -- (144.26.2.137) with Microsoft SMTP Server (TLS) id 15.0.1178.4; Wed, 25 Jan 24, 41 -- 2017 16:46:45 -0500 24, 41 -- Received: from WCUXCHP08.PASSHE.LCL ([144.26.2.144]) by WCUXCHP08.PASSHE.LCL 24, 41 -- ([144.26.2.144]) with mapi id 15.00.1178.000; Wed, 25 Jan 2017 16:46:45 -0500 24, 41 -- From: "Monson, Frederick" {FMonson-at-wcupa.edu} 24, 41 -- To: "jcarroll-at-murraystate.edu" {jcarroll-at-murraystate.edu} 24, 41 -- CC: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 24, 41 -- Subject: RE: [Microscopy] TEM negative staining issues 24, 41 -- Thread-Topic: [Microscopy] TEM negative staining issues 24, 41 -- X-ASG-Orig-Subj: RE: [Microscopy] TEM negative staining issues 24, 41 -- Thread-Index: AQHSd0v9UudivfJbB0253DI8Or0XKaFJr4Dw 24, 41 -- Date: Wed, 25 Jan 2017 21:46:44 +0000 24, 41 -- Message-ID: {d3698c21c4ed40f0989836c0a643a53d-at-WCUXCHP08.PASSHE.LCL} 24, 41 -- References: {201701252040.v0PKexdF013610-at-microscopy.com} 24, 41 -- In-Reply-To: {201701252040.v0PKexdF013610-at-microscopy.com} 24, 41 -- Accept-Language: en-US 24, 41 -- Content-Language: en-US 24, 41 -- X-MS-Has-Attach: 24, 41 -- X-MS-TNEF-Correlator: 24, 41 -- x-ms-exchange-transport-fromentityheader: Hosted 24, 41 -- x-originating-ip: [144.26.2.59] 24, 41 -- Content-Type: text/plain; charset="us-ascii" 24, 41 -- MIME-Version: 1.0 24, 41 -- X-Barracuda-Connect: UNKNOWN[144.26.2.137] 24, 41 -- X-Barracuda-Start-Time: 1485380837 24, 41 -- X-Barracuda-Encrypted: ECDHE-RSA-AES256-SHA384 24, 41 -- X-Barracuda-URL: https://SPAMCONTROL.WCUPA.EDU:443/cgi-mod/mark.cgi 24, 41 -- X-Virus-Scanned: by bsmtpd at WCUPA.EDU 24, 41 -- X-Barracuda-BRTS-Status: 1 24, 41 -- Content-Transfer-Encoding: 8bit 24, 41 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v0PLg6JN026497 ==============================End of - Headers==============================
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From rotator-at-mg-style.cn Wed Jan 25 23:29:13 2017 Return-Path: {rotator-at-mg-style.cn} Received: from mg-style.cn ([43.225.199.130]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v0Q5TBkA017975 for {microscopylistserverarchive6-at-microscopy.com} ; Wed, 25 Jan 2017 23:29:12 -0600 DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; s=key79; d=mg-style.cn; h=Message-ID:Date:Reply-To:From:User-Agent:MIME-Version:To:Subject:Content-Type:Content-Transfer-Encoding; b=ZZCGIFIsrQT++VG8fU4afLjmO34GlRzpL+0iy9cnSpBdogAFeDfpwruXFb6SiwpvY0JYWit9+IA6HA11F+uXaJb0k3QO345/Fl3STl5FYhsQomQ/i636BCasolH0prCal7UUbIy+rXeuSmpm3GoHPgoXVJWcttIw/YfkEnuLv0I=; Message-ID: {075C9ED5.7ECDC7CF-at-mg-style.cn}
Dear All, i would like to ask for your recommendation for a temperature logger. Probably a reasonable sensitivity, acuracy and precision and is able to monitor live using the TEM pc. I am not sure if such system will interfere any performance of the TEM. If you encounter such problem, is it possible to share your experience? I do know some logger has wifi/bluetooth capability, will this interfere the TEM performances well? whiCurrently we have a temperature logger but its not live and we have to extract the data out from time to time which is very troublesome.
Cheers, Yee Yan, Tay FACTS Lab NTU Singapore
==============================Original Headers============================== 2, 29 -- From rongchigram79-at-yahoo.com.sg Thu Jan 26 02:46:34 2017 2, 29 -- Received: from nm11-vm7.bullet.mail.sg3.yahoo.com (nm11-vm7.bullet.mail.sg3.yahoo.com [106.10.148.246]) 2, 29 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0Q8kWah020307 2, 29 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Jan 2017 02:46:33 -0600 2, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com.sg; s=s2048; t=1485420703; bh=6qN0PhRcJB09HCGFUyxPVhRd9guTg7iqD/EvlCLXJnc=; h=Date:From:Reply-To:To:Subject:References:From:Subject; b=c6Tt98oAxObfHMm7NLdKppiPxSprMYMCNhxNxQTkQv+xr75cFypmnoyMLKl8r30x18LEw9GLUhMnPW82J25ERJs/ajq1vyc1WlPmtfvPjCS8k4OlIJbNZ3quSlKM6Od4dO7HyIKBzYURwOBFQeRMlFve0yWRJawwZtmqO5UDxGPXQvEuaH6evssrmmhauYKeQTMn77OFwg3MGY7e8Brz6J9WW6DmXVi2B1pLUxJLUO2mEKl3ONcYuPAwyCFVlyCQoiWKi0/iyq/tm5VT2OYwdXQaQysl/no1tEdu992hoP8+mgck+PRFCfnAgPVWEQi1UhQiGTDysMMfulSsOUn2WA== 2, 29 -- Received: from [106.10.166.120] by nm11.bullet.mail.sg3.yahoo.com with NNFMP; 26 Jan 2017 08:51:43 -0000 2, 29 -- Received: from [106.10.151.250] by tm9.bullet.mail.sg3.yahoo.com with NNFMP; 26 Jan 2017 08:51:43 -0000 2, 29 -- Received: from [127.0.0.1] by omp1021.mail.sg3.yahoo.com with NNFMP; 26 Jan 2017 08:51:43 -0000 2, 29 -- X-Yahoo-Newman-Property: ymail-3 2, 29 -- X-Yahoo-Newman-Id: 394027.14600.bm-at-omp1021.mail.sg3.yahoo.com 2, 29 -- X-YMail-OSG: T0dUrMgVM1n6BVCBg3fxDjF5_oRmAxZT6IbMR7dm9HNOUedWZEnHbUKQk1J9kcB 2, 29 -- gvD6_0N0LbFHB20r4LPwA3jRlCbL1ChtHEJRgE2xSH2zYxjhFoYLGyMR3iQ7A2t1Jv.Wvs1LU.oW 2, 29 -- f._lJX8Zn1IsrG78tyRBPMDYndQYUl738yddERkQ4BCSJIXemHd2BVMGQGO74NOAWhwqhfWDck4Q 2, 29 -- 2ERUrDJdT0NOY8LrPgQIV7_T17Czr68KLBW2AdmAxu8y9romJ3W86r1ZZyO7m68VN4H8JUOXPjZ4 2, 29 -- fPbqB9et10u_YcROp6cw5MKXIuZHjEOeeiZJDqgDDFePjah7mBM26amiwMmaTzdMChJaJCtag4Fb 2, 29 -- cD87sLIE4.rkQe7brzkrpB3vZUz2kOdUcd9eR4qNjguBOmX4wdoWCeAz67M.blc0b8i9czGQbt_z 2, 29 -- OTYSbAyRzz1HnoeHLfWA7yh_SVKjzMPItsWYOD5hjD4JONHi4QTXTWoz5f.IkXhMMePMV1xDsOdI 2, 29 -- savgutwlmG_GjZL..H5GIkLrK6SsGUnDzQgW_EYMZeOc3zg-- 2, 29 -- Received: from jws600002.mail.sg3.yahoo.com by sendmailws118.mail.sg3.yahoo.com; Thu, 26 Jan 2017 08:51:42 +0000; 1485420702.563 2, 29 -- Date: Thu, 26 Jan 2017 08:51:42 +0000 (UTC) 2, 29 -- From: YY YY {rongchigram79-at-yahoo.com.sg} 2, 29 -- Reply-To: YY YY {rongchigram79-at-yahoo.com.sg} 2, 29 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 2, 29 -- Message-ID: {1775741231.1160911.1485420702386-at-mail.yahoo.com} 2, 29 -- Subject: Temperature Logger to Recommend 2, 29 -- MIME-Version: 1.0 2, 29 -- Content-Type: text/plain; charset=UTF-8 2, 29 -- Content-Transfer-Encoding: 7bit 2, 29 -- References: {1775741231.1160911.1485420702386.ref-at-mail.yahoo.com} ==============================End of - Headers==============================
you may want to talk to Guenter Resch of Nexperion. He might be able to help. His email address: guenter.resch-at-nexperion.net
Cheers,
Jan Leunissen
} On 26/01/2017, at 21:46, rongchigram79-at-yahoo.com.sg wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear All, i would like to ask for your recommendation for a temperature logger. Probably a reasonable sensitivity, acuracy and precision and is able to monitor live using the TEM pc. I am not sure if such system will interfere any performance of the TEM. If you encounter such problem, is it possible to share your experience? I do know some logger has wifi/bluetooth capability, will this interfere the TEM performances well? whiCurrently we have a temperature logger but its not live and we have to extract the data out from time to time which is very troublesome. } } Cheers, } Yee Yan, Tay } FACTS Lab } NTU } Singapore } } ==============================Original Headers============================== } 2, 29 -- From rongchigram79-at-yahoo.com.sg Thu Jan 26 02:46:34 2017 } 2, 29 -- Received: from nm11-vm7.bullet.mail.sg3.yahoo.com (nm11-vm7.bullet.mail.sg3.yahoo.com [106.10.148.246]) } 2, 29 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0Q8kWah020307 } 2, 29 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Jan 2017 02:46:33 -0600 } 2, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com.sg; s=s2048; t=1485420703; bh=6qN0PhRcJB09HCGFUyxPVhRd9guTg7iqD/EvlCLXJnc=; h=Date:From:Reply-To:To:Subject:References:From:Subject; b=c6Tt98oAxObfHMm7NLdKppiPxSprMYMCNhxNxQTkQv+xr75cFypmnoyMLKl8r30x18LEw9GLUhMnPW82J25ERJs/ajq1vyc1WlPmtfvPjCS8k4OlIJbNZ3quSlKM6Od4dO7HyIKBzYURwOBFQeRMlFve0yWRJawwZtmqO5UDxGPXQvEuaH6evssrmmhauYKeQTMn77OFwg3MGY7e8Brz6J9WW6DmXVi2B1pLUxJLUO2mEKl3ONcYuPAwyCFVlyCQoiWKi0/iyq/tm5VT2OYwdXQaQysl/no1tEdu992hoP8+mgck+PRFCfnAgPVWEQi1UhQiGTDysMMfulSsOUn2WA== } 2, 29 -- Received: from [106.10.166.120] by nm11.bullet.mail.sg3.yahoo.com with NNFMP; 26 Jan 2017 08:51:43 -0000 } 2, 29 -- Received: from [106.10.151.250] by tm9.bullet.mail.sg3.yahoo.com with NNFMP; 26 Jan 2017 08:51:43 -0000 } 2, 29 -- Received: from [127.0.0.1] by omp1021.mail.sg3.yahoo.com with NNFMP; 26 Jan 2017 08:51:43 -0000 } 2, 29 -- X-Yahoo-Newman-Property: ymail-3 } 2, 29 -- X-Yahoo-Newman-Id: 394027.14600.bm-at-omp1021.mail.sg3.yahoo.com } 2, 29 -- X-YMail-OSG: T0dUrMgVM1n6BVCBg3fxDjF5_oRmAxZT6IbMR7dm9HNOUedWZEnHbUKQk1J9kcB } 2, 29 -- gvD6_0N0LbFHB20r4LPwA3jRlCbL1ChtHEJRgE2xSH2zYxjhFoYLGyMR3iQ7A2t1Jv.Wvs1LU.oW } 2, 29 -- f._lJX8Zn1IsrG78tyRBPMDYndQYUl738yddERkQ4BCSJIXemHd2BVMGQGO74NOAWhwqhfWDck4Q } 2, 29 -- 2ERUrDJdT0NOY8LrPgQIV7_T17Czr68KLBW2AdmAxu8y9romJ3W86r1ZZyO7m68VN4H8JUOXPjZ4 } 2, 29 -- fPbqB9et10u_YcROp6cw5MKXIuZHjEOeeiZJDqgDDFePjah7mBM26amiwMmaTzdMChJaJCtag4Fb } 2, 29 -- cD87sLIE4.rkQe7brzkrpB3vZUz2kOdUcd9eR4qNjguBOmX4wdoWCeAz67M.blc0b8i9czGQbt_z } 2, 29 -- OTYSbAyRzz1HnoeHLfWA7yh_SVKjzMPItsWYOD5hjD4JONHi4QTXTWoz5f.IkXhMMePMV1xDsOdI } 2, 29 -- savgutwlmG_GjZL..H5GIkLrK6SsGUnDzQgW_EYMZeOc3zg-- } 2, 29 -- Received: from jws600002.mail.sg3.yahoo.com by sendmailws118.mail.sg3.yahoo.com; Thu, 26 Jan 2017 08:51:42 +0000; 1485420702.563 } 2, 29 -- Date: Thu, 26 Jan 2017 08:51:42 +0000 (UTC) } 2, 29 -- From: YY YY {rongchigram79-at-yahoo.com.sg} } 2, 29 -- Reply-To: YY YY {rongchigram79-at-yahoo.com.sg} } 2, 29 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} } 2, 29 -- Message-ID: {1775741231.1160911.1485420702386-at-mail.yahoo.com} } 2, 29 -- Subject: Temperature Logger to Recommend } 2, 29 -- MIME-Version: 1.0 } 2, 29 -- Content-Type: text/plain; charset=UTF-8 } 2, 29 -- Content-Transfer-Encoding: 7bit } 2, 29 -- References: {1775741231.1160911.1485420702386.ref-at-mail.yahoo.com} } ==============================End of - Headers==============================
==============================Original Headers============================== 7, 35 -- From leunissen-at-aurion.nl Thu Jan 26 03:04:45 2017 7, 35 -- Received: from nm14-vm5.access.bullet.mail.bf1.yahoo.com (nm14-vm5.access.bullet.mail.bf1.yahoo.com [216.109.115.20]) 7, 35 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0Q94i60006442 7, 35 -- for {microscopy-at-microscopy.com} ; Thu, 26 Jan 2017 03:04:45 -0600 7, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1485421797; bh=MH/TbUKNWbFVdYQai4502bsM24Lj+D9xdpYMa9OG9Jk=; h=Subject:From:In-Reply-To:Date:Cc:References:To:From:Subject; b=qJkC1rNRU9AIz+CPjXW9fwKkebRbAY/w8z73vGeDFazB1Td7JrPOawGBsAqTC27lZPIhKBfaZgzT7qL7ufLadUnJ3aMVl8dCr4/rVt1zhZdZBd5c7sGGLm3PXPXwvL9lDOwun/+IyMuer+lEDFYpkmuvclW3uX0g32lgKBOQ9BY7MO5kbZYSAFW/5WJ4q1gNW6bBZbeVbxSJRpqqgOYStehDZE/gsOnDv/BiAXQB/h4m2iOLEnRdIEj7i0m4Y2OAYYlJ0b1vSYuq4EQ+YV423ltnPjUxRdLmBgjlQkkpUxdpFJ4bvjXtHxgwrargUNuWM7L/ZbvZ/pVZuz6uavmeuw== 7, 35 -- Received: from [66.196.81.166] by nm14.access.bullet.mail.bf1.yahoo.com with NNFMP; 26 Jan 2017 09:09:57 -0000 7, 35 -- Received: from [98.138.226.241] by tm12.access.bullet.mail.bf1.yahoo.com with NNFMP; 26 Jan 2017 09:09:57 -0000 7, 35 -- Received: from [127.0.0.1] by smtp112.sbc.mail.ne1.yahoo.com with NNFMP; 26 Jan 2017 09:09:57 -0000 7, 35 -- X-Yahoo-Newman-Id: 357734.1479.bm-at-smtp112.sbc.mail.ne1.yahoo.com 7, 35 -- X-Yahoo-Newman-Property: ymail-3 7, 35 -- X-YMail-OSG: 0F1VpCEVM1nIpyLz.CM3YAYJV9VYBi9E1dByjbdxnVmvA8O 7, 35 -- tibdXHrRAJI.EPAH23y1f0daPVQcKBnktrcIP1XAVXfpl2pRJbFSquJPOk5R 7, 35 -- YJqETXq_KG5Cs0zagyx1o.E4sgbVlsmxeVcUIjlqw.yMTR92vSwl8Uz_83uM 7, 35 -- BPmtnGQcIvniVIESNvma3WiPS1aUbUxpPsufOZY7maZ59fAo.9_90kbr0WhN 7, 35 -- MFRBitUXxidgZPtRCn.9dYbK3qanp9_FQtvVsAzAZ8cNRn6LnwurRbcdYKS1 7, 35 -- iwSVZS6.tnWojLetAZSa.nTkcT9uvd7ZEfwlZ6OMNIpi3G1Ybo0hE4F1TBY4 7, 35 -- TZdiiQJcdROJ2N9NkkPUWm4tDXJ6Eijml8f3_sTbkKZHR4cBXTAWQn75FRFa 7, 35 -- bwIDBfT97AZ0vcttYNiHr.k99IseROoCBEJ8oHISfkb3f22RUY5qyg8TXtZA 7, 35 -- c6yqzQ3FmYP3fITOTcgP5wUz3av9YH_BFyCioy6wgCxs4yvFx7.hRsxxGFV5 7, 35 -- _2Q6EYo0RFQBwUpLhCbu70OUUpGl.UBfHpRkrBXm1JYYCdoIHAhTyfZ1An9k 7, 35 -- VedqP.7PnQA_bKQ-- 7, 35 -- X-Yahoo-SMTP: E1TINkOswBCOniPaNnp23qBGQpLN4KsU.cr0KdlJlWhF 7, 35 -- Subject: Re: [Microscopy] Temperature Logger to Recommend 7, 35 -- Mime-Version: 1.0 (Mac OS X Mail 9.3 \(3124\)) 7, 35 -- Content-Type: text/plain; charset=us-ascii 7, 35 -- From: Jan Leunissen {leunissen-at-aurion.nl} 7, 35 -- In-Reply-To: {201701260846.v0Q8kpIx020562-at-microscopy.com} 7, 35 -- Date: Thu, 26 Jan 2017 22:09:50 +1300 7, 35 -- Cc: microscopy-at-microscopy.com 7, 35 -- Message-Id: {9C33975F-2ABE-41B8-B3B9-67FCDAF1EBEB-at-aurion.nl} 7, 35 -- References: {201701260846.v0Q8kpIx020562-at-microscopy.com} 7, 35 -- To: rongchigram79-at-yahoo.com.sg 7, 35 -- X-Mailer: Apple Mail (2.3124) 7, 35 -- Content-Transfer-Encoding: 8bit 7, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v0Q94i60006442 ==============================End of - Headers==============================
I have used Extech RH520A temperature/humidity data logger in FIB room and was very pleased with it in all respects. Initial setup required some programming, but features and display/download capabilities were excellent:
http://www.extech.com/display/?id=14702
Valery Ray www.linkedin.com/in/valeryray {http://www.linkedin.com/in/valeryray} ============================== PBS&T, MEO Engineering Company 290 Broadway, Suite 298 Methuen, MA 01844, USA Phone: +1-978-305-0479 {tel:%2B1-978-305-0479} - leave a message Mobie: +1-978-305-0479 {tel:%2B1-978-305-0479} - leave a message E-mail: vray-at-partbeamsystech.com {mailto:vray-at-partbeamsystech.com} Web: www.partbeamsystech.com {http://www.partbeamsystech.com} Web: www.freudlabs.com {http://www.freudlabs.com}
On 1/26/2017 4:06 AM, leunissen-at-aurion.nl wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe --http://www.microscopy.com/MicroscopyListserver } On-Line Helphttp://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello, } } you may want to talk to Guenter Resch of Nexperion. He might be able to help. } His email address:guenter.resch-at-nexperion.net } } Cheers, } } Jan Leunissen } } } On 26/01/2017, at 21:46,rongchigram79-at-yahoo.com.sg wrote: } } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe --http://www.microscopy.com/MicroscopyListserver } } On-Line Helphttp://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Dear All, i would like to ask for your recommendation for a temperature logger. Probably a reasonable sensitivity, acuracy and precision and is able to monitor live using the TEM pc. I am not sure if such system will interfere any performance of the TEM. If you encounter such problem, is it possible to share your experience? I do know some logger has wifi/bluetooth capability, will this interfere the TEM performances well? whiCurrently we have a temperature logger but its not live and we have to extract the data out from time to time which is very troublesome. } } } } Cheers, } } Yee Yan, Tay } } FACTS Lab } } NTU } } Singapore } } } } ==============================Original Headers============================== } } 2, 29 -- Fromrongchigram79-at-yahoo.com.sg Thu Jan 26 02:46:34 2017 } } 2, 29 -- Received: from nm11-vm7.bullet.mail.sg3.yahoo.com (nm11-vm7.bullet.mail.sg3.yahoo.com [106.10.148.246]) } } 2, 29 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0Q8kWah020307 } } 2, 29 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Jan 2017 02:46:33 -0600 } } 2, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com.sg; s=s2048; t=1485420703; bh=6qN0PhRcJB09HCGFUyxPVhRd9guTg7iqD/EvlCLXJnc=; h=Date:From:Reply-To:To:Subject:References:From:Subject; b=c6Tt98oAxObfHMm7NLdKppiPxSprMYMCNhxNxQTkQv+xr75cFypmnoyMLKl8r30x18LEw9GLUhMnPW82J25ERJs/ajq1vyc1WlPmtfvPjCS8k4OlIJbNZ3quSlKM6Od4dO7HyIKBzYURwOBFQeRMlFve0yWRJawwZtmqO5UDxGPXQvEuaH6evssrmmhauYKeQTMn77OFwg3MGY7e8Brz6J9WW6DmXVi2B1pLUxJLUO2mEKl3ONcYuPAwyCFVlyCQoiWKi0/iyq/tm5VT2OYwdXQaQysl/no1tEdu992hoP8+mgck+PRFCfnAgPVWEQi1UhQiGTDysMMfulSsOUn2WA== } } 2, 29 -- Received: from [106.10.166.120] by nm11.bullet.mail.sg3.yahoo.com with NNFMP; 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Thu, 26 Jan 2017 08:51:42 +0000; 1485420702.563 } } 2, 29 -- Date: Thu, 26 Jan 2017 08:51:42 +0000 (UTC) } } 2, 29 -- From: YY YY {rongchigram79-at-yahoo.com.sg} } } 2, 29 -- Reply-To: YY YY {rongchigram79-at-yahoo.com.sg} } } 2, 29 -- To:"Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} } } 2, 29 -- Message-ID: {1775741231.1160911.1485420702386-at-mail.yahoo.com} } } 2, 29 -- Subject: Temperature Logger to Recommend } } 2, 29 -- MIME-Version: 1.0 } } 2, 29 -- Content-Type: text/plain; charset=UTF-8 } } 2, 29 -- Content-Transfer-Encoding: 7bit } } 2, 29 -- References: {1775741231.1160911.1485420702386.ref-at-mail.yahoo.com} } } ==============================End of - Headers============================== } } } ==============================Original Headers============================== } 7, 35 -- Fromleunissen-at-aurion.nl Thu Jan 26 03:04:45 2017 } 7, 35 -- Received: from nm14-vm5.access.bullet.mail.bf1.yahoo.com (nm14-vm5.access.bullet.mail.bf1.yahoo.com [216.109.115.20]) } 7, 35 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0Q94i60006442 } 7, 35 -- for {microscopy-at-microscopy.com} ; 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charset=us-ascii } 7, 35 -- From: Jan Leunissen {leunissen-at-aurion.nl} } 7, 35 -- In-Reply-To: {201701260846.v0Q8kpIx020562-at-microscopy.com} } 7, 35 -- Date: Thu, 26 Jan 2017 22:09:50 +1300 } 7, 35 -- Cc:microscopy-at-microscopy.com } 7, 35 -- Message-Id: {9C33975F-2ABE-41B8-B3B9-67FCDAF1EBEB-at-aurion.nl} } 7, 35 -- References: {201701260846.v0Q8kpIx020562-at-microscopy.com} } 7, 35 -- To:rongchigram79-at-yahoo.com.sg } 7, 35 -- X-Mailer: Apple Mail (2.3124) } 7, 35 -- Content-Transfer-Encoding: 8bit } 7, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v0Q94i60006442 } ==============================End of - Headers==============================
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==============================Original Headers============================== 8, 20 -- From eikonika-at-otenet.gr Fri Jan 27 03:23:23 2017 8, 20 -- Received: from chimaera.otenet.gr (smtp-out32.otenet.gr [83.235.69.32]) 8, 20 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0R9NMZg031024 8, 20 -- for {microscopy-at-microscopy.com} ; Fri, 27 Jan 2017 03:23:22 -0600 8, 20 -- Received: from ozymandias (ppp-2-87-177-161.home.otenet.gr [2.87.177.161]) 8, 20 -- by chimaera.otenet.gr (ESMTP) with ESMTPSA 8, 20 -- for {microscopy-at-microscopy.com} ; Fri, 27 Jan 2017 11:28:36 +0200 (EET) 8, 20 -- From: "Yorgos Nikas" {eikonika-at-otenet.gr} 8, 20 -- To: {microscopy-at-microscopy.com} 8, 20 -- Subject: Jeol 5600LV strip aperture sizes 8, 20 -- Date: Fri, 27 Jan 2017 11:28:37 +0200 8, 20 -- Message-ID: {000301d2787f$bd396280$37ac2780$-at-otenet.gr} 8, 20 -- MIME-Version: 1.0 8, 20 -- Content-Type: text/plain; 8, 20 -- charset="iso-8859-1" 8, 20 -- X-Mailer: Microsoft Outlook 14.0 8, 20 -- Thread-Index: AdJ4f10Kl1+ws/8YRteDf2k9m9yX8w== 8, 20 -- Content-Language: en-us 8, 20 -- Content-Transfer-Encoding: 8bit 8, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v0R9NMZg031024 ==============================End of - Headers==============================
From annepenn4-at-gmail.com Fri Jan 27 06:05:02 2017 Return-Path: {annepenn4-at-gmail.com} Received: from gmail.com ([61.76.233.68]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v0RC50j2030377 for {microscopylistserverarchive6-at-microscopy.com} ; Fri, 27 Jan 2017 06:05:01 -0600 Message-ID: {7E9065EF.1F21205C-at-gmail.com}
} I recommend a temperature sensor from Vernier.com. Their line of Go Direct sensors connect to a laptop by USB, are dirt cheap ($39 for the one I have), and come with a very nice free software package (I have never seen it crash!) for recording your measurements. I see now they have options for wireless with iOS devices. Have fun! } } } } John Mardinly, Ph.D., P.E., Retired ASU } } } } } On Jan 26, 2017, at 1:59 AM, rongchigram79-at-yahoo.com.sg wrote: } } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.com_MicroscopyListserver&d=DQIBAg&c=AGbYxfJbXK67KfXyGqyv2Ejiz41FqQuZFk4A-1IxfAU&r=MAuGvnWTcVQkxORgQD0QS50ZicPM3Nw-61ygSK-LNEQ&m=w7SxKtx8upRjcBdFpASg01gW7K2Na0pxXE9_phnExns&s=3kp26tRvY-UtqU1X55VcwWuqjnnEqOcxRc3Jhq-UwKw&e= } } On-Line Help https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.com_MicroscopyListserver_FAQ.html&d=DQIBAg&c=AGbYxfJbXK67KfXyGqyv2Ejiz41FqQuZFk4A-1IxfAU&r=MAuGvnWTcVQkxORgQD0QS50ZicPM3Nw-61ygSK-LNEQ&m=w7SxKtx8upRjcBdFpASg01gW7K2Na0pxXE9_phnExns&s=WCAC1lAnxktP17l4ddVAVdqEv21AGNCZxrUNpilB-XM&e= } } ---------------------------------------------------------------------------- } } } } Dear All, i would like to ask for your recommendation for a temperature logger. Probably a reasonable sensitivity, acuracy and precision and is able to monitor live using the TEM pc. I am not sure if such system will interfere any performance of the TEM. If you encounter such problem, is it possible to share your experience? I do know some logger has wifi/bluetooth capability, will this interfere the TEM performances well? whiCurrently we have a temperature logger but its not live and we have to extract the data out from time to time which is very troublesome. } } } } Cheers, } } Yee Yan, Tay } } FACTS Lab } } NTU } } Singapore } } } } ==============================Original Headers============================== } } 2, 29 -- From rongchigram79-at-yahoo.com.sg Thu Jan 26 02:46:34 2017 } } 2, 29 -- Received: from nm11-vm7.bullet.mail.sg3.yahoo.com (nm11-vm7.bullet.mail.sg3.yahoo.com [106.10.148.246]) } } 2, 29 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0Q8kWah020307 } } 2, 29 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Jan 2017 02:46:33 -0600 } } 2, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com.sg; s=s2048; t=1485420703; bh=6qN0PhRcJB09HCGFUyxPVhRd9guTg7iqD/EvlCLXJnc=; h=Date:From:Reply-To:To:Subject:References:From:Subject; b=c6Tt98oAxObfHMm7NLdKppiPxSprMYMCNhxNxQTkQv+xr75cFypmnoyMLKl8r30x18LEw9GLUhMnPW82J25ERJs/ajq1vyc1WlPmtfvPjCS8k4OlIJbNZ3quSlKM6Od4dO7HyIKBzYURwOBFQeRMlFve0yWRJawwZtmqO5UDxGPXQvEuaH6evssrmmhauYKeQTMn77OFwg3MGY7e8Brz6J9WW6DmXVi2B1pLUxJLUO2mEKl3ONcYuPAwyCFVlyCQoiWKi0/iyq/tm5VT2OYwdXQaQysl/no1tEdu992hoP8+mgck+PRFCfnAgPVWEQi1UhQiGTDysMMfulSsOUn2WA== } } 2, 29 -- Received: from [106.10.166.120] by nm11.bullet.mail.sg3.yahoo.com with NNFMP; 26 Jan 2017 08:51:43 -0000 } } 2, 29 -- Received: from [106.10.151.250] by tm9.bullet.mail.sg3.yahoo.com with NNFMP; 26 Jan 2017 08:51:43 -0000 } } 2, 29 -- Received: from [127.0.0.1] by omp1021.mail.sg3.yahoo.com with NNFMP; 26 Jan 2017 08:51:43 -0000 } } 2, 29 -- X-Yahoo-Newman-Property: ymail-3 } } 2, 29 -- X-Yahoo-Newman-Id: 394027.14600.bm-at-omp1021.mail.sg3.yahoo.com } } 2, 29 -- X-YMail-OSG: T0dUrMgVM1n6BVCBg3fxDjF5_oRmAxZT6IbMR7dm9HNOUedWZEnHbUKQk1J9kcB } } 2, 29 -- gvD6_0N0LbFHB20r4LPwA3jRlCbL1ChtHEJRgE2xSH2zYxjhFoYLGyMR3iQ7A2t1Jv.Wvs1LU.oW } } 2, 29 -- f._lJX8Zn1IsrG78tyRBPMDYndQYUl738yddERkQ4BCSJIXemHd2BVMGQGO74NOAWhwqhfWDck4Q } } 2, 29 -- 2ERUrDJdT0NOY8LrPgQIV7_T17Czr68KLBW2AdmAxu8y9romJ3W86r1ZZyO7m68VN4H8JUOXPjZ4 } } 2, 29 -- fPbqB9et10u_YcROp6cw5MKXIuZHjEOeeiZJDqgDDFePjah7mBM26amiwMmaTzdMChJaJCtag4Fb } } 2, 29 -- cD87sLIE4.rkQe7brzkrpB3vZUz2kOdUcd9eR4qNjguBOmX4wdoWCeAz67M.blc0b8i9czGQbt_z } } 2, 29 -- OTYSbAyRzz1HnoeHLfWA7yh_SVKjzMPItsWYOD5hjD4JONHi4QTXTWoz5f.IkXhMMePMV1xDsOdI } } 2, 29 -- savgutwlmG_GjZL..H5GIkLrK6SsGUnDzQgW_EYMZeOc3zg-- } } 2, 29 -- Received: from jws600002.mail.sg3.yahoo.com by sendmailws118.mail.sg3.yahoo.com; Thu, 26 Jan 2017 08:51:42 +0000; 1485420702.563 } } 2, 29 -- Date: Thu, 26 Jan 2017 08:51:42 +0000 (UTC) } } 2, 29 -- From: YY YY {rongchigram79-at-yahoo.com.sg} } } 2, 29 -- Reply-To: YY YY {rongchigram79-at-yahoo.com.sg} } } 2, 29 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} } } 2, 29 -- Message-ID: {1775741231.1160911.1485420702386-at-mail.yahoo.com} } } 2, 29 -- Subject: Temperature Logger to Recommend } } 2, 29 -- MIME-Version: 1.0 } } 2, 29 -- Content-Type: text/plain; charset=UTF-8 } } 2, 29 -- Content-Transfer-Encoding: 7bit } } 2, 29 -- References: {1775741231.1160911.1485420702386.ref-at-mail.yahoo.com} } } ==============================End of - Headers============================== }
==============================Original Headers============================== 4, 96 -- From John.Mardinly-at-asu.edu Fri Jan 27 10:30:47 2017 4, 96 -- Received: from bcnetw1.asu.edu (bcnetw1.asu.edu [149.169.2.71]) 4, 96 -- by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v0RGUkGD009204 4, 96 -- for {Microscopy-at-Microscopy.com} ; Fri, 27 Jan 2017 10:30:46 -0600 4, 96 -- X-ASG-Debug-ID: 1485534963-0d12c77a271103e0001-FOsErg 4, 96 -- Received: from ppnetw4.asu.edu (ppnetw4.asu.edu [10.118.52.71]) by bcnetw1.asu.edu with ESMTP id WVTnEuBXs2QOD2Jm (version=TLSv1.2 cipher=ECDHE-RSA-AES256-GCM-SHA384 bits=256 verify=NO); Fri, 27 Jan 2017 09:36:03 -0700 (MST) 4, 96 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu 4, 96 -- Received: from pps.filterd (ppnetw4.asu.edu [127.0.0.1]) 4, 96 -- by ppnetw4.asu.edu (8.16.0.17/8.16.0.17) with SMTP id v0RGZc1V021245; 4, 96 -- Fri, 27 Jan 2017 09:36:03 -0700 4, 96 -- Received: from exhubw02.asurite.ad.asu.edu (exnswvpx-10-118-52-65.inre.asu.edu [10.118.52.65]) 4, 96 -- by ppnetw4.asu.edu with ESMTP id 28893d03g7-1 4, 96 -- (version=TLSv1 cipher=ECDHE-RSA-AES256-SHA bits=256 verify=NOT); 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I have several units from ITWatchdogs. Never had any problem and they email and text me and the building managers when there is a thermal issue out of range. Interface is a standard web browser.
Scott Whittaker Lab Manager Imaging P.O. Box 37012 MRC-104 Room W150 Washington, DC 20013-7012 w 202.633.0891 whittaks-at-si.edu
SMITHSONIAN INSTITUTION NATIONAL MUSEUM OF NATURAL HISTORY
-----Original Message----- X-from: John.Mardinly-at-asu.edu [mailto:John.Mardinly-at-asu.edu] Sent: Friday, January 27, 2017 11:38 AM To: Whittaker, Scott {WHITTAKS-at-si.edu}
} I recommend a temperature sensor from Vernier.com. Their line of Go Direct sensors connect to a laptop by USB, are dirt cheap ($39 for the one I have), and come with a very nice free software package (I have never seen it crash!) for recording your measurements. I see now they have options for wireless with iOS devices. Have fun! } } } } John Mardinly, Ph.D., P.E., Retired ASU } } } } } On Jan 26, 2017, at 1:59 AM, rongchigram79-at-yahoo.com.sg wrote: } } } } } } } } } } --------------------------------------------------------------------- } } ------- The Microscopy ListServer -- CoSponsor: The Microscopy } } Society of America To Subscribe/Unsubscribe -- } } https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.co } } m_MicroscopyListserver&d=DQIBAg&c=AGbYxfJbXK67KfXyGqyv2Ejiz41FqQuZFk4 } } A-1IxfAU&r=MAuGvnWTcVQkxORgQD0QS50ZicPM3Nw-61ygSK-LNEQ&m=w7SxKtx8upRj } } cBdFpASg01gW7K2Na0pxXE9_phnExns&s=3kp26tRvY-UtqU1X55VcwWuqjnnEqOcxRc3 } } Jhq-UwKw&e= On-Line Help } } https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.co } } m_MicroscopyListserver_FAQ.html&d=DQIBAg&c=AGbYxfJbXK67KfXyGqyv2Ejiz4 } } 1FqQuZFk4A-1IxfAU&r=MAuGvnWTcVQkxORgQD0QS50ZicPM3Nw-61ygSK-LNEQ&m=w7S } } xKtx8upRjcBdFpASg01gW7K2Na0pxXE9_phnExns&s=WCAC1lAnxktP17l4ddVAVdqEv2 } } 1AGNCZxrUNpilB-XM&e= } } --------------------------------------------------------------------- } } ------- } } } } Dear All, i would like to ask for your recommendation for a temperature logger. Probably a reasonable sensitivity, acuracy and precision and is able to monitor live using the TEM pc. I am not sure if such system will interfere any performance of the TEM. If you encounter such problem, is it possible to share your experience? I do know some logger has wifi/bluetooth capability, will this interfere the TEM performances well? whiCurrently we have a temperature logger but its not live and we have to extract the data out from time to time which is very troublesome. } } } } Cheers, } } Yee Yan, Tay } } FACTS Lab } } NTU } } Singapore } } } } ==============================Original } } Headers============================== } } 2, 29 -- From rongchigram79-at-yahoo.com.sg Thu Jan 26 02:46:34 2017 2, } } 29 -- Received: from nm11-vm7.bullet.mail.sg3.yahoo.com (nm11-vm7.bullet.mail.sg3.yahoo.com [106.10.148.246]) } } 2, 29 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0Q8kWah020307 } } 2, 29 -- for {Microscopy-at-microscopy.com} ; Thu, 26 Jan 2017 02:46:33 -0600 } } 2, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } } d=yahoo.com.sg; s=s2048; t=1485420703; } } bh=6qN0PhRcJB09HCGFUyxPVhRd9guTg7iqD/EvlCLXJnc=; } } h=Date:From:Reply-To:To:Subject:References:From:Subject; } } b=c6Tt98oAxObfHMm7NLdKppiPxSprMYMCNhxNxQTkQv+xr75cFypmnoyMLKl8r30x18L } } Ew9GLUhMnPW82J25ERJs/ajq1vyc1WlPmtfvPjCS8k4OlIJbNZ3quSlKM6Od4dO7HyIKB } } zYURwOBFQeRMlFve0yWRJawwZtmqO5UDxGPXQvEuaH6evssrmmhauYKeQTMn77OFwg3MG } } Y7e8Brz6J9WW6DmXVi2B1pLUxJLUO2mEKl3ONcYuPAwyCFVlyCQoiWKi0/iyq/tm5VT2O } } YwdXQaQysl/no1tEdu992hoP8+mgck+PRFCfnAgPVWEQi1UhQiGTDysMMfulSsOUn2WA= } } = 2, 29 -- Received: from [106.10.166.120] by } } nm11.bullet.mail.sg3.yahoo.com with NNFMP; 26 Jan 2017 08:51:43 -0000 } } 2, 29 -- Received: from [106.10.151.250] by } } tm9.bullet.mail.sg3.yahoo.com with NNFMP; 26 Jan 2017 08:51:43 -0000 } } 2, 29 -- Received: from [127.0.0.1] by omp1021.mail.sg3.yahoo.com } } with NNFMP; 26 Jan 2017 08:51:43 -0000 2, 29 -- } } X-Yahoo-Newman-Property: ymail-3 2, 29 -- X-Yahoo-Newman-Id: } } 394027.14600.bm-at-omp1021.mail.sg3.yahoo.com } } 2, 29 -- X-YMail-OSG: } } T0dUrMgVM1n6BVCBg3fxDjF5_oRmAxZT6IbMR7dm9HNOUedWZEnHbUKQk1J9kcB } } 2, 29 -- } } gvD6_0N0LbFHB20r4LPwA3jRlCbL1ChtHEJRgE2xSH2zYxjhFoYLGyMR3iQ7A2t1Jv.Wv } } s1LU.oW 2, 29 -- } } f._lJX8Zn1IsrG78tyRBPMDYndQYUl738yddERkQ4BCSJIXemHd2BVMGQGO74NOAWhwqh } } fWDck4Q 2, 29 -- } } 2ERUrDJdT0NOY8LrPgQIV7_T17Czr68KLBW2AdmAxu8y9romJ3W86r1ZZyO7m68VN4H8J } } UOXPjZ4 2, 29 -- } } fPbqB9et10u_YcROp6cw5MKXIuZHjEOeeiZJDqgDDFePjah7mBM26amiwMmaTzdMChJaJ } } Ctag4Fb 2, 29 -- } } cD87sLIE4.rkQe7brzkrpB3vZUz2kOdUcd9eR4qNjguBOmX4wdoWCeAz67M.blc0b8i9c } } zGQbt_z 2, 29 -- } } OTYSbAyRzz1HnoeHLfWA7yh_SVKjzMPItsWYOD5hjD4JONHi4QTXTWoz5f.IkXhMMePMV } } 1xDsOdI 2, 29 -- savgutwlmG_GjZL..H5GIkLrK6SsGUnDzQgW_EYMZeOc3zg-- } } 2, 29 -- Received: from jws600002.mail.sg3.yahoo.com by } } sendmailws118.mail.sg3.yahoo.com; Thu, 26 Jan 2017 08:51:42 +0000; } } 1485420702.563 2, 29 -- Date: Thu, 26 Jan 2017 08:51:42 +0000 (UTC) } } 2, 29 -- From: YY YY {rongchigram79-at-yahoo.com.sg} 2, 29 -- Reply-To: } } YY YY {rongchigram79-at-yahoo.com.sg} 2, 29 -- To: } } "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 2, 29 -- } } Message-ID: {1775741231.1160911.1485420702386-at-mail.yahoo.com} } } 2, 29 -- Subject: Temperature Logger to Recommend 2, 29 -- } } MIME-Version: 1.0 2, 29 -- Content-Type: text/plain; charset=UTF-8 2, } } 29 -- Content-Transfer-Encoding: 7bit 2, 29 -- References: } } {1775741231.1160911.1485420702386.ref-at-mail.yahoo.com} } } ==============================End of - } } Headers============================== }
==============================Original Headers============================== 4, 96 -- From John.Mardinly-at-asu.edu Fri Jan 27 10:30:47 2017 4, 96 -- Received: from bcnetw1.asu.edu (bcnetw1.asu.edu [149.169.2.71]) 4, 96 -- by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v0RGUkGD009204 4, 96 -- for {Microscopy-at-Microscopy.com} ; Fri, 27 Jan 2017 10:30:46 -0600 4, 96 -- X-ASG-Debug-ID: 1485534963-0d12c77a271103e0001-FOsErg 4, 96 -- Received: from ppnetw4.asu.edu (ppnetw4.asu.edu [10.118.52.71]) by bcnetw1.asu.edu with ESMTP id WVTnEuBXs2QOD2Jm (version=TLSv1.2 cipher=ECDHE-RSA-AES256-GCM-SHA384 bits=256 verify=NO); Fri, 27 Jan 2017 09:36:03 -0700 (MST) 4, 96 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu 4, 96 -- Received: from pps.filterd (ppnetw4.asu.edu [127.0.0.1]) 4, 96 -- by ppnetw4.asu.edu (8.16.0.17/8.16.0.17) with SMTP id v0RGZc1V021245; 4, 96 -- Fri, 27 Jan 2017 09:36:03 -0700 4, 96 -- Received: from exhubw02.asurite.ad.asu.edu (exnswvpx-10-118-52-65.inre.asu.edu [10.118.52.65]) 4, 96 -- by ppnetw4.asu.edu with ESMTP id 28893d03g7-1 4, 96 -- (version=TLSv1 cipher=ECDHE-RSA-AES256-SHA bits=256 verify=NOT); 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==============================Original Headers============================== 16, 28 -- From WHITTAKS-at-si.edu Fri Jan 27 10:42:14 2017 16, 28 -- Received: from si-mailout04.si.edu (si-mailout04.si.edu [160.111.103.178]) 16, 28 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0RGgDWY019647 16, 28 -- for {microscopy-at-microscopy.com} ; Fri, 27 Jan 2017 10:42:14 -0600 16, 28 -- Received: from SI-MSECHT03.US.SINET.SI.EDU (si-msecht03.us.sinet.si.edu [160.111.49.155]) 16, 28 -- by si-mailout04.si.edu (Postfix) with ESMTP id 5DE89E63AC 16, 28 -- for {microscopy-at-microscopy.com} ; Fri, 27 Jan 2017 11:47:31 -0500 (EST) 16, 28 -- Received: from SI-MSEDAG04.US.SINET.SI.EDU ([169.254.4.29]) by 16, 28 -- SI-MSECHT03.US.SINET.SI.EDU ([160.111.49.149]) with mapi id 14.03.0319.002; 16, 28 -- Fri, 27 Jan 2017 11:47:30 -0500 16, 28 -- From: "Whittaker, Scott" {WHITTAKS-at-si.edu} 16, 28 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 16, 28 -- Subject: RE: [Microscopy] Re: Temperature Logger to Recommend 16, 28 -- Thread-Topic: [Microscopy] Re: Temperature Logger to Recommend 16, 28 -- Thread-Index: AQHSeLu+b9YsafiZcEa7ntj9NbEuk6FMhzNw 16, 28 -- Date: Fri, 27 Jan 2017 16:47:29 +0000 16, 28 -- Message-ID: {5D4F637E5C599649A11693E3B6EAC6293FC5CA1E-at-si-msedag04.US.SINET.SI.EDU} 16, 28 -- References: {201701271632.v0RGWo8p010904-at-microscopy.com} 16, 28 -- In-Reply-To: {201701271632.v0RGWo8p010904-at-microscopy.com} 16, 28 -- Accept-Language: en-US 16, 28 -- Content-Language: en-US 16, 28 -- X-MS-Has-Attach: 16, 28 -- X-MS-TNEF-Correlator: 16, 28 -- x-originating-ip: [160.111.49.170] 16, 28 -- Content-Type: text/plain; charset="us-ascii" 16, 28 -- MIME-Version: 1.0 16, 28 -- Content-Transfer-Encoding: 8bit 16, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v0RGgDWY019647 ==============================End of - Headers==============================
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Message: Colleagues, we regret to note the passing of Sterling Newberry a long time member of the microscopy community whose passion for microscopy and education will be missed.
https://en.wikipedia.org/wiki/Sterling_Newberry
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From amazon-at-boronamore.org Sun Jan 29 15:42:49 2017 Return-Path: {amazon-at-boronamore.org} Received: from bor.boronamore.org (bor.boronamore.org [192.232.196.16] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0TLgmMe000693 for {microscopylistserverarchive6-at-microscopy.com} ; Sun, 29 Jan 2017 15:42:49 -0600 DKIM-Signature: v=1; a=rsa-sha256; q=dns/txt; c=relaxed/relaxed; d=boronamore.org; s=default; h=Content-Transfer-Encoding:Content-Type: MIME-Version:Message-ID:From:Date:Subject:To:Sender:Reply-To:Cc:Content-ID: Content-Description:Resent-Date:Resent-From:Resent-Sender:Resent-To:Resent-Cc :Resent-Message-ID:In-Reply-To:References:List-Id:List-Help:List-Unsubscribe: List-Subscribe:List-Post:List-Owner:List-Archive; bh=LN7r7oyJsuymYL6J3iYMfus2P3D/RNe4SauojXPvtbQ=; b=mNYrtOzP4f1LRcYSkK7ARwYjN+ jV7aLhaCclxn/BsgycDUpCzs03CedcYjoROL5Zb1j83tkdMLpcal4atXPc4/Kwr/46OW1CbKlmUqa 3hjFecC50wVJGXItw4xmm9hnf6Ny0DTFQymDRBnIiFnlwMlSzXGVSk4sMRLUX3rIqo1YKa8Q/2FsG oJZvJwAYqs+WTlqba/dRLLSt6icohqVSW0V9vZwE3mrkzJhl2bTx+CBDULZDlI8LIZo3co1eDVhv6 Kfgrw3zq5N48j3UVrS1CeRT0kE76Pfaq1nsfOOTr01TBOBRXsHPb4BhrZkrHIhCb4JKi8fh1tuPsY skBL678g==; Received: from boss by bor.boronamore.org with local (Exim 4.87) (envelope-from {amazon-at-boronamore.org} ) id 1cXxKX-00080D-QJ for microscopylistserverarchive6-at-microscopy.com; Sun, 29 Jan 2017 15:48:13 -0600 To: microscopylistserverarchive6-at-microscopy.com
Thanks to all who responded. We have found a new home for this system. If arrangements fall through, we will select another choice from the previous inquiries.
Regards, Jim
-- Jim Passmore Principal Scientist, R&D
Sealed Air Corporation P: 864.433.2927 100 Rogers Bridge Rd., Bldg A Duncan, SC 29334
==============================Original Headers============================== 4, 34 -- From james.passmore-at-sealedair.com Mon Jan 30 07:48:09 2017 4, 34 -- Received: from mail-wm0-f42.google.com (mail-wm0-f42.google.com [74.125.82.42]) 4, 34 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0UDm92g025482 4, 34 -- for {Microscopy-at-microscopy.com} ; Mon, 30 Jan 2017 07:48:09 -0600 4, 34 -- Received: by mail-wm0-f42.google.com with SMTP id v77so48478912wmv.0 4, 34 -- for {Microscopy-at-microscopy.com} ; Mon, 30 Jan 2017 05:53:37 -0800 (PST) 4, 34 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 34 -- d=sealedair.com; s=google; 4, 34 -- h=mime-version:from:date:message-id:subject:to; 4, 34 -- bh=Qj3/kicE/70sVrrMA6dU8waubZBL4iIMs7+rWbKeS0s=; 4, 34 -- b=A78d4rAgimFjQcXCSi7tdLJZCLNLxSLLxP/9U1X2RtP+H3Mgze44ysZunEDOG4FPBu 4, 34 -- L5y2hA+rb3zdjj09nE2nt7f+9htjeRJHkUScJJIZRR00KzAQDtBGZI4i0y9+VDwB9Mu7 4, 34 -- YOV5tOVBKY4IcdiZgbrWD+IUYpZTSo5msaGcU= 4, 34 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 34 -- d=1e100.net; s=20161025; 4, 34 -- h=x-gm-message-state:mime-version:from:date:message-id:subject:to; 4, 34 -- bh=Qj3/kicE/70sVrrMA6dU8waubZBL4iIMs7+rWbKeS0s=; 4, 34 -- b=Kg4X/o0rTV5GMuwbvaj9ZcfIBgygeP/injyHafawqKdBZNbHZyk5+ANc5MhotLi2tN 4, 34 -- 7bdSlXwEqsbbWnxzSLWzrxragCJt8E4I6/hiMBxT95F7otkMmXTgWfHN1jZ1nvgVF639 4, 34 -- Kty6yHtXxMAJHSieIC/263ksbHZi/Ar9DBhAdkE3BjB5etHjvg2cfD7HVnL3CUYeanGi 4, 34 -- Jqo3LNbosrUTphYYrQyeDj7WjuIqfx8s4FF8atm0uXcH59yKP8vNyYQz+FW0NYt9QZLR 4, 34 -- zkFFy9z06ldJMLizdE5GRLO/hGeZFqkZ9asHe5YEZvCogiAgDSYAKz2ZkIgLKlEP9Ysb 4, 34 -- 8t9A== 4, 34 -- X-Gm-Message-State: AIkVDXJWheMIck3Tgrca/nCBUCYf4+v1tE4Erq52N2GfkgHMB+RAUhCmhioz9KI2EICmZyu9xGNnna5m0ovY5ap4 4, 34 -- X-Received: by 10.223.129.4 with SMTP id 4mr22600514wrm.27.1485784416628; Mon, 4, 34 -- 30 Jan 2017 05:53:36 -0800 (PST) 4, 34 -- MIME-Version: 1.0 4, 34 -- Received: by 10.194.246.161 with HTTP; Mon, 30 Jan 2017 05:53:06 -0800 (PST) 4, 34 -- From: Jim Passmore {james.passmore-at-sealedair.com} 4, 34 -- Date: Mon, 30 Jan 2017 08:53:06 -0500 4, 34 -- Message-ID: {CAGV-iYavoM+mJi-4CFWv_TRvfh0Taypq+RXnq_HCoq+50+Z=Sg-at-mail.gmail.com} 4, 34 -- Subject: XPS/ESCA system has new home 4, 34 -- To: microscopy {Microscopy-at-microscopy.com} 4, 34 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
From smithdiana157-at-gmail.com Mon Jan 30 11:59:01 2017 Return-Path: {smithdiana157-at-gmail.com} Received: from gmail.com (mail.dongnamdc.com [222.97.39.100] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v0UHwxZ0019571 for {microscopylistserverarchive6-at-microscopy.com} ; Mon, 30 Jan 2017 11:59:00 -0600 Message-ID: {AB757720.37CE6E18-at-gmail.com}
X-from: leandro.lemgrubersoares-at-glasgow.ac.uk
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Message: Dear All, does anyone know if there is a membrane permeable dye or probe that recognises HA-tag protein in live imaging?
Thanks!
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} Begin forwarded message: } } From: Nancy Gray {ngray-at-grc.org} } Subject: Re: [3dem] GRC Locations } Date: January 31, 2017 at 19:19:24 GMT+1 } To: "Smith, Phillip R." {smithp01-at-nyumc.org} , Marin van Heel {marin.vanheel-at-googlemail.com} } Cc: Henning Stahlberg {henning.stahlberg-at-unibas.ch} , Microscopy {Microscopy-at-microscopy.com} , 3dem {3dem-at-ncmir.ucsd.edu} , "CCPEM-at-JISCMAIL.AC.UK" {CCPEM-at-JISCMAIL.AC.UK} } } Folks, } Could you kindly forward my response to your network since my attempt was rejected since I am not authorized. I am getting many emails on the issue and would like all to know GRC is formulating a response. Many thanks, } Nancy } } Nancy Ryan Gray, PhD } Gordon Research Conferences, President and Chief Executive Officer } 512 Liberty Lane } West Kingston, RI 02892 } 401-360-1521 } www.grc.org } } } } On Jan 31, 2017, at 11:33 AM, Nancy Gray {ngray-at-grc.org} wrote: } } } } Dear Henning, Ulrike, Stefan, Marin, Ross and members of the 3dem mailing list, } } } } Thank you for your input, it is indeed a difficult situation. I have scheduled a conference call with the GRC Board of Trustees Executive Committee and GRC Legal Counsel to address these concerns and will get back to you shortly. } } Thanks for your support, patience and understanding. } } Nancy } } } } } } } } Nancy Ryan Gray, PhD, President and Chief Executive Officer } } Gordon Research Conferences } } 512 Liberty Lane, West Kingston, RI 02892-1502 } } Phone: 401-360-1521 l Fax: 401-783-7644 } } Email: ngray-at-grc.org l Web: http://www.grc.org
[]
} } } From: Henning Stahlberg {henning.stahlberg-at-unibas.ch} } } } Subject: [3dem] GRC Locations } } } Date: January 30, 2017 at 21:08:10 GMT+1 } } } To: "Nancy R. Gray" {NGray-at-grc.org} } } } Cc: Microscopy {Microscopy-at-microscopy.com} , 3dem {3dem-at-ncmir.ucsd.edu} } } } } } } Dear Nancy, } } } } } } Science is open and international. The US is currently not, unfortunately. } } } Until that situation is resolved, may I ask you to consider moving the upcoming GRC conferences to openly accessible locations, such as international conference sites as in Cancun in Mexico or in other countries. } } } This would allow fair and equal access of scientist from all countries and religions to your conferences, and prevent a boycott movement from forming. } } } Your conferences in Europe and Asia are a great start. } } } } } } With best wishes, } } } } } } Henning Stahlberg. } } } } } } } } } Henning Stahlberg, PhD } } } Prof. for Structural Biology, C-CINA, Biozentrum, University Basel } } } Mattenstrasse 26 | D-BSSE | WRO-1058 | CH-4058 Basel | Switzerland } } } http://c-cina.org | Tel. +41-61-387 32 62 } } }
==============================Original Headers============================== 6, 35 -- From henning.stahlberg-at-unibas.ch Tue Jan 31 14:38:40 2017 6, 35 -- Received: from mx2-priv.urz.unibas.ch (mx2-priv.urz.unibas.ch [131.152.226.165]) 6, 35 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v0VKcd6O014824 6, 35 -- for {Microscopy-at-microscopy.com} ; Tue, 31 Jan 2017 14:38:40 -0600 6, 35 -- Received: from localhost (localhost [127.0.0.1]) 6, 35 -- by mx2-priv.urz.unibas.ch (Postfix) with ESMTP id C28DE22019F; 6, 35 -- Tue, 31 Jan 2017 21:44:11 +0100 (CET) 6, 35 -- X-Virus-Scanned: amavisd-new at unibas.ch 6, 35 -- Received: from mx2-priv.urz.unibas.ch ([131.152.226.165]) 6, 35 -- by localhost (mx2-mgnt.urz.unibas.ch [127.0.0.1]) (amavisd-new, port 10024) 6, 35 -- with LMTP id JEJbqur-zeLN; Tue, 31 Jan 2017 21:44:11 +0100 (CET) 6, 35 -- Received: from URZ-HT-CAS-4.urz.unibas.ch (urz-ht-cas-4.urz.unibas.ch [131.152.8.134]) 6, 35 -- by mx2-priv.urz.unibas.ch (Postfix) with ESMTPS id B1686220188; 6, 35 -- Tue, 31 Jan 2017 21:44:11 +0100 (CET) 6, 35 -- Received: from URZ-MBX-1.urz.unibas.ch ([131.152.8.141]) by 6, 35 -- URZ-HT-CAS-4.urz.unibas.ch ([fe80::1c8f:5331:992b:ec37%15]) with mapi id 6, 35 -- 14.03.0319.002; Tue, 31 Jan 2017 21:44:11 +0100 6, 35 -- From: Henning Stahlberg {henning.stahlberg-at-unibas.ch} 6, 35 -- To: 3dem {3dem-at-ncmir.ucsd.edu} , Microscopy {Microscopy-at-microscopy.com} 6, 35 -- Subject: Fwd: [3dem] GRC Locations 6, 35 -- Thread-Topic: [3dem] GRC Locations 6, 35 -- Thread-Index: AQHSezSUjSU5xLihGUqfyWAj4PWHXA== 6, 35 -- Date: Tue, 31 Jan 2017 20:44:11 +0000 6, 35 -- Message-ID: {715A7637-36FD-489D-A5C1-D2C10BACA42D-at-unibas.ch} 6, 35 -- References: {EBA6B95B-6ED4-4414-A50C-CB35C4BFFE48-at-grc.org} 6, 35 -- Accept-Language: en-US, de-CH 6, 35 -- Content-Language: en-US 6, 35 -- X-MS-Has-Attach: 6, 35 -- X-MS-TNEF-Correlator: 6, 35 -- x-originating-ip: [131.152.226.242] 6, 35 -- Content-Type: text/plain; charset="Windows-1252" 6, 35 -- Content-ID: {93EE1C68CEF34441BCD0B44F6BFDC3D4-at-exch.unibas.ch} 6, 35 -- MIME-Version: 1.0 6, 35 -- Content-Transfer-Encoding: 8bit 6, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v0VKcd6O014824 ==============================End of - Headers==============================
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Email: erosen-at-mednet.ucla.edu Name: Eric Rosen Organization: UCLA Medical Center Title-Subject: [Filtered] Histo / EM position open Message: Hi all,
We have a 50% Histo/50% EM position open at UCLA hospital.
See job description here: http://www.uclahealthcareers.org/all-jobs/Histotechnologist-II/H88333 Login Host: 149.142.103.151 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
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One day too late, but anyway happy new year to all, as it is my first post of the year !
I'm looking if someone could have soon written a bash script (or else working on linux system) to convert in batch mode EDAX spectra from the .spc file format to .emsa/msa. The "Export" function of the TEAM software has only the .spc format as output, and the "Send_To_Folder" function is far too time consuming for saving a project part with tenth of spectra, in particular as it doesn't keep the original names of the data.
I tried Spectrum Viewer but I didn't bring it to work on linux (via Wine, but I'm very binary with Wine : it works or doesn't work... I've no time to learn to configure it !).
Fortunately, DTSA-II reads the .spc format and can save in emsa, what allows me to work. But DTSA has not the goal to do batch format conversions !
Thanks to all
Jacques
J. Faerber IPCMS-DSI Institut de Physique et Chimie des Matériaux de Strasbourg Département Surfaces et Interfaces 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
The open-source HyperSpy software (www.hyperspy.org) can do what you are looking for. EDAX file reading (both .spd and .spc) has been added in the latest development builds (and should be included in the next minor release -- v1.2). If you cannot wait for that, setting up a copy of the development version isn't too difficult if you have some proficiency with Python and git (http://hyperspy.org/hyperspy-doc/current/user_guide/install.html#development-version).
Good luck!
- Josh
NB: Any mention of commercial products or software is for information only; it does not imply recommendation or endorsement by NIST.
-- Joshua Taillon, Ph.D. Materials Measurement Science Division National Institute of Standards and Technology
-----Original Message----- X-from: jacques.faerber-at-ipcms.unistra.fr [mailto:jacques.faerber-at-ipcms.unistra.fr] Sent: Wednesday, February 1, 2017 4:42 AM To: Taillon, Joshua A. (Fed) {joshua.taillon-at-nist.gov}
Hi all
One day too late, but anyway happy new year to all, as it is my first post of the year !
I'm looking if someone could have soon written a bash script (or else working on linux system) to convert in batch mode EDAX spectra from the .spc file format to .emsa/msa. The "Export" function of the TEAM software has only the .spc format as output, and the "Send_To_Folder" function is far too time consuming for saving a project part with tenth of spectra, in particular as it doesn't keep the original names of the data.
I tried Spectrum Viewer but I didn't bring it to work on linux (via Wine, but I'm very binary with Wine : it works or doesn't work... I've no time to learn to configure it !).
Fortunately, DTSA-II reads the .spc format and can save in emsa, what allows me to work. But DTSA has not the goal to do batch format conversions !
Thanks to all
Jacques
J. Faerber IPCMS-DSI Institut de Physique et Chimie des Matériaux de Strasbourg Département Surfaces et Interfaces 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
Unless someone have already written and would share the script you need for converting .spc to .emsa, it should be possible to automate sequential opening of files within a folder by DTSA-II, saving data in another format, and repeating the operation until all files are processed with one of GUI scripting tools. I am using WinBatch (US$100 from http://www.windowware.com/, no connection just a happy user) for file processing purposes, although in different then yours application. There is also AutoIt which is free from www.autoitscript.com, but I did not try it. Not aware of similar GUI scripting tools for Linux, but would be surprised if they didn't exist as well.
Valery Ray www.linkedin.com/in/valeryray ============================== PBS&T, MEO Engineering Company 290 Broadway, Suite 298 Methuen, MA 01844, USA Phone: +1-978-305-0479 - leave a message Mobie: +1-978-305-0479 - leave a message E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 2/1/2017 4:20 AM, jacques.faerber-at-ipcms.unistra.fr wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi all } } One day too late, but anyway happy new year to all, as it is my first } post of the year ! } } I'm looking if someone could have soon written a bash script (or else } working on linux system) to convert in batch mode EDAX spectra from the } .spc file format to .emsa/msa. } The "Export" function of the TEAM software has only the .spc format as } output, and the "Send_To_Folder" function is far too time consuming for } saving a project part with tenth of spectra, in particular as it doesn't } keep the original names of the data. } } I tried Spectrum Viewer but I didn't bring it to work on linux (via } Wine, but I'm very binary with Wine : it works or doesn't work... I've } no time to learn to configure it !). } } Fortunately, DTSA-II reads the .spc format and can save in emsa, what } allows me to work. But DTSA has not the goal to do batch format } conversions ! } } Thanks to all } } Jacques } } J. Faerber } IPCMS-DSI } Institut de Physique et Chimie des Matériaux de Strasbourg } Département Surfaces et Interfaces } 23, rue de Loess ; BP43 } 67034 Strasbourg CEDEX 2 } France } } Tel 00 33(0)3 88 10 71 01 } Fax 00 33(0)3 88 10 72 48 } E-mail : Jacques.Faerber-at-ipcms.unistra.fr } } } ==============================Original Headers============================== } 10, 26 -- From jacques.faerber-at-ipcms.unistra.fr Wed Feb 1 03:19:35 2017 } 10, 26 -- Received: from webmail.ipcms.fr (webmail.ipcms.fr [130.79.210.2]) } 10, 26 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v119JY3g019013 } 10, 26 -- for {microscopy-at-microscopy.com} ; Wed, 1 Feb 2017 03:19:35 -0600 } 10, 26 -- Received: from localhost (localhost [127.0.0.1]) } 10, 26 -- by webmail.ipcms.fr (Postfix) with ESMTP id A48AFE718E } 10, 26 -- for {microscopy-at-microscopy.com} ; Wed, 1 Feb 2017 10:25:08 +0100 (CET) } 10, 26 -- X-Virus-Scanned: Debian amavisd-new at webmail.ipcms.fr } 10, 26 -- Received: from webmail.ipcms.fr ([127.0.0.1]) } 10, 26 -- by localhost (webmail.ipcms.fr [127.0.0.1]) (amavisd-new, port 10024) } 10, 26 -- with ESMTP id SZ6XiuzgMIwg for {microscopy-at-microscopy.com} ; } 10, 26 -- Wed, 1 Feb 2017 10:25:07 +0100 (CET) } 10, 26 -- Received: from wing.ipcms1.ipcms.unistra.fr (wing.u-strasbg.fr [130.79.153.228]) } 10, 26 -- (Authenticated sender: jfaerber-at-ipcms.unistra.fr) } 10, 26 -- by webmail.ipcms.fr (Postfix) with ESMTPSA id 99B62E70F5 } 10, 26 -- for {microscopy-at-microscopy.com} ; Wed, 1 Feb 2017 10:25:07 +0100 (CET) } 10, 26 -- From: jacques faerber {jacques.faerber-at-ipcms.unistra.fr} } 10, 26 -- Subject: EDAX .spc to .emsa format batch conversion } 10, 26 -- To: microscopy-at-microscopy.com } 10, 26 -- Message-ID: {a7b5d9c4-ce93-8c03-ff5c-48ed6903ec72-at-ipcms.unistra.fr} } 10, 26 -- Date: Wed, 1 Feb 2017 10:25:07 +0100 } 10, 26 -- User-Agent: Mozilla/5.0 (X11; Linux x86_64; rv:45.0) Gecko/20100101 } 10, 26 -- Thunderbird/45.5.1 } 10, 26 -- MIME-Version: 1.0 } 10, 26 -- Content-Type: text/plain; charset=utf-8; format=flowed } 10, 26 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers============================== }
==============================Original Headers============================== 4, 37 -- From vray-at-partbeamsystech.com Wed Feb 1 10:28:11 2017 4, 37 -- Received: from nm31.bullet.mail.gq1.yahoo.com (nm31.bullet.mail.gq1.yahoo.com [98.136.217.14]) 4, 37 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v11GSBXn012214 4, 37 -- for {microscopy-at-microscopy.com} ; Wed, 1 Feb 2017 10:28:11 -0600 4, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1485966826; bh=Nq3d7mdXxW5hEuoMWPME5ggCOt4MTExdXYfZW8KzXvM=; h=Subject:To:References:From:Cc:Date:In-Reply-To:From:Subject; b=OUJMbdQ34+2x91oNmSiKyRkYyRuLgujCYaPkZMTZR4qzCbxo8GSgMno7elxw4Fu5QYAYAL8187krgMZJeFXgJF6zin3WpTi+xH0ef75/m4GuvTCns54IYpemjPU93z49V5cZGe2i50rszon0DDaFX+NYxOe/xEYUiq+3gcTCHcVqh7f1wAV2QdvYTxorZqBB42yHB7nUCKW2VhUZH4jWhp608in3HiyjhFgJkc9PXzjSuvTVAiRrhzaJwP5qpkwyY6Fw0gpIEREpd0C/zKAYVHTl2CNxWs8xNrfhpmTD2rQR7iGJ41iQSZUriotkyBfw65JrAivu7oS9yUZKigXfQw== 4, 37 -- Received: from [127.0.0.1] by nm31.bullet.mail.gq1.yahoo.com with NNFMP; 01 Feb 2017 16:33:46 -0000 4, 37 -- Received: from [98.137.12.62] by nm31.bullet.mail.gq1.yahoo.com with NNFMP; 01 Feb 2017 16:31:00 -0000 4, 37 -- Received: from [98.139.170.182] by tm7.bullet.mail.gq1.yahoo.com with NNFMP; 01 Feb 2017 16:31:00 -0000 4, 37 -- Received: from [68.142.230.76] by tm25.bullet.mail.bf1.yahoo.com with NNFMP; 01 Feb 2017 16:31:00 -0000 4, 37 -- Received: from [127.0.0.1] by smtp233.mail.bf1.yahoo.com with NNFMP; 01 Feb 2017 16:31:00 -0000 4, 37 -- X-Yahoo-Newman-Id: 16718.77480.bm-at-smtp233.mail.bf1.yahoo.com 4, 37 -- X-Yahoo-Newman-Property: ymail-4 4, 37 -- X-YMail-OSG: ooZZ4MMVM1lfEWO_ftr1.OYqw12hW63xZJm86rq8uS1NVZH 4, 37 -- rTvHFCcBqe4eW0HAoG1n0BLC4miV02Le7i2fCfAmn7mHynCQaOXw6Q3Sq836 4, 37 -- sL__wB7kVZ8t5YiFnfnIBj0frk9ToMiC9Y1ZWtAaTdscattcfqhbpheU7uUP 4, 37 -- D6ikZYDZAxpHiBsHp2edv8bYL_h1HA1U5V9CG1oWfeaWHjj7UYWzbk0oab0s 4, 37 -- v1eyVpu..74LK6bu0mhrmj0LA0sX.BCGT70XlYwfeZ.2cyHe6nfx7VfMSzee 4, 37 -- 4z9jXmG5x_kb1pjwrofPeGL2CqGmfkzJAoQX6q69KbLidClhLwHTNuM1JWEw 4, 37 -- .H0Ayf76w6S.VljFFU6Cy07XVcrlZJBMTlUH7b8B6SykNyZ7uaxDOvhh1RKr 4, 37 -- UBdtxtDxLDd5mCxIAPLynn1c4S_dalpbXlqk_eWBwYiaAysbBbm9nBHPATeq 4, 37 -- aNY4JBk5s2ojP_pcdwSVFWH_MzYNrXK4mGM4_OEhDZnan0DRUR88GI6nM_N4 4, 37 -- RHrgrJ9yJEo5O55ObmArN9tWKyOIqDbrzsGW7If88tN3UbKcq6F32OKW2P4i 4, 37 -- NotAaZhrOVRpeUdPZobSq 4, 37 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 4, 37 -- Subject: Re: [Microscopy] EDAX .spc to .emsa format batch conversion 4, 37 -- To: jacques.faerber-at-ipcms.unistra.fr 4, 37 -- References: {201702010920.v119KpQx020159-at-microscopy.com} 4, 37 -- From: Valery Ray {vray-at-partbeamsystech.com} 4, 37 -- Cc: microscopy-at-microscopy.com 4, 37 -- Message-ID: {e64844c1-23be-5627-649d-03bae2544509-at-partbeamsystech.com} 4, 37 -- Date: Wed, 1 Feb 2017 11:28:22 -0500 4, 37 -- User-Agent: Mozilla/5.0 (Windows NT 10.0; WOW64; rv:45.0) Gecko/20100101 4, 37 -- Thunderbird/45.7.0 4, 37 -- MIME-Version: 1.0 4, 37 -- In-Reply-To: {201702010920.v119KpQx020159-at-microscopy.com} 4, 37 -- Content-Type: text/plain; charset=windows-1252; format=flowed 4, 37 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
Jacques Faerber asked about a script for the conversion of EDAX .spc to msa format.
DTSA-II has a nice scripting language (jython) that permits this. One can also apply a DTSA calibrated detector instance to the spectrum. I sent Jacques amd earlier version of this this morning. I want to make certain that the formatting stays correct, so I created a public gist on GitHub. You can obtain the script at:
Hello Everyone, Our 1 meg Gatan Camera (model 780 Duel vision) on our TEM is dying and we want to give it a transplant. I'm looking for a cooling fan.
It's the 780.PL6FA model, if that helps. It's from the turn of the century and if you have a one that's broken and the fan works or you have a spare fan or know of a replacement fan, I sure would like to hear from you.
Thanks in advance........ Frank Karl ARDL 330-794-6600
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==============================Original Headers============================== 6, 28 -- From frank_karl-at-ardl.com Wed Feb 1 14:04:51 2017 6, 28 -- Received: from cal1-mh745b.smtproutes.com (cal1-mh745b.smtproutes.com [208.70.89.155]) 6, 28 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v11K4oeI031609 6, 28 -- for {microscopy-at-microscopy.com} ; Wed, 1 Feb 2017 14:04:51 -0600 6, 28 -- X-Katharion-ID: 1485979808.49884.cal1-mh745 6, 28 -- Received: from mail.ardl.com ([74.207.215.218]) by 6, 28 -- cal1-mh745.smtproutes.com [(192.69.16.67)] with ESMTP via TCP 6, 28 -- (TLSv1/TLS_RSA_WITH_AES_128_CBC_SHA); 01 Feb 2017 20:10:08 +0000 6, 28 -- Received: from exchange2k7.ad.ardl.com ([fe80::d57a:b38c:8f17:9f73]) by 6, 28 -- exchange2k7.ad.ardl.com ([fe80::d57a:b38c:8f17:9f73%15]) with mapi; Wed, 1 6, 28 -- Feb 2017 15:10:07 -0500 6, 28 -- From: Frank Karl {frank_karl-at-ardl.com} 6, 28 -- To: "Microscopy Listserver (microscopy-at-microscopy.com)" 6, 28 -- {microscopy-at-microscopy.com} 6, 28 -- Date: Wed, 1 Feb 2017 15:10:06 -0500 6, 28 -- Subject: Our TEM needs a cool breeze..... 6, 28 -- Thread-Topic: Our TEM needs a cool breeze..... 6, 28 -- Thread-Index: AdJ8xy5dvYNL9ahiQhCUYpRgYVG00w== 6, 28 -- Message-ID: {0445DA8FFC235F4CBDAF9BACCF79E1212CC3813F86-at-exchange2k7.ad.ardl.com} 6, 28 -- Accept-Language: en-US 6, 28 -- Content-Language: en-US 6, 28 -- X-MS-Has-Attach: 6, 28 -- X-MS-TNEF-Correlator: 6, 28 -- acceptlanguage: en-US 6, 28 -- Content-Type: text/plain; charset="us-ascii" 6, 28 -- MIME-Version: 1.0 6, 28 -- Content-Transfer-Encoding: 8bit 6, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v11K4oeI031609 ==============================End of - Headers==============================
I am an accomplished Electron Microscopy Technician, supervisor, and research specialist with 25 years experience working in both clinical and research environments looking for a position where my acquired skills can be used for professional advancement.
Posting of "looking for a position" emails has never been permitted. Please do not do this.
There are job boards, one specifically hosted and run by the Microscopy Society of America http://jobs.microscopy.org/ that fulfill this role.
Nestor Your Friendly Neighborhood SysCop
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Email: nlamine6-at-hotmail.com Name: naitbouda
Organization: cdta
Title-Subject: [Filtered] strip
Message: Hello
hello the size of jeol 5600LV and JEOL 6360LV IS THE SAME the Jeol strip apertures 3 holes are/ 20micron -30um-100um
you can use the size of Jeol strip aperture 9.0x2.35x0.10mm (platinium-Iridium)
Part:67001-10-20-100
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Thank you for your efforts to disseminate your communications to a larger audience. This is an important issue from many perspectives and I imagine most subscribers to this list will be very interested in how the GRC responds. I would suggest that scientific societies all ought to have a measured and well thought out position on the current political situation, and that those positions be made abundantly clear world-wide. From my personal perspective, I feel boycotts are unhelpful in resolving larger issues, especially when the boycotting group has little to no true leverage other than to shoot itself in the foot.
Robert J. Palmer Jr., PhD National Institute of Dental and Craniofacial Research National Institutes of Health Building 30, Room 331 Bethesda MD 20892 301-594-0025
On 1/31/17, 4:00 PM, "henning.stahlberg-at-unibas.ch" {henning.stahlberg-at-unibas.ch} wrote:
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Email: lon.nelson-at-leica-microsystems.com Name: Lon Nelson
Message: Leica Microsystems is currently seeking a commissioned Sales Agent or Dealer in the Southeast USA for our Electron Microscopy Sample Preparation solutions.
https://tinyurl.com/leicaEMprep
Interested parties should contact Lon Nelson, Director of Sales, at lon.nelson-at-leica-microsystems.com.
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Lon Nelson Director of Sales Microscopy lon.nelson-at-leica-microsystems.com M +1 224-628-2467 | F +1 847-607-3160 Leica Microsystems, Inc. 1700 Leider Ln | Buffalo Grove, IL 60089 (USA)
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The deadline for paper submission for M&M 2017 is less than two weeks away and as you are making plans to attend the St. Louis meetings (Aug. 6-10), please don't forget about MSA's student bursary program. Its purpose is to encourage students to attend the meetings by helping to defray some of the costs while giving them an opportunity to meet and interact with the established microscopy community.
The student bursaries will be paid $10 an hour to work for ~20 hours during the meeting or pre-meeting events. The jobs involve such things as providing support in the different symposia, staffing the volunteer office; newsletter distribution, and helping with vendor tutorial sign-up.
Once the program has been finalized, each registered bursary will be contacted and given the chance to choose the times and activities where they would like to help. There is an added bonus of $10 cash for each morning and/or afternoon session worked to assist with meals and a meeting shirt.
If anyone would like to participate in the bursary program, please check the box "I wish to apply for a student bursary" in section 2 of the registration form. Applicants for the bursaries must be members of MSA or MAS, enrolled as students at a recognized educational institution, and pay their registration fee. Bursary space is limited, so sign-up early.
If anyone has any questions about the bursary program, or would like to participate, please contact Amanda (alawrence-at-i2at.msstate.edu).
Also don't forget about the opportunities for meeting awards!
Amanda Lawrence Institute for Imaging and Analytical Technologies Mississippi State University 662-325-7998 alawrence-at-i2at.msstate.edu
==============================Original Headers============================== 9, 32 -- From ALawrence-at-i2at.msstate.edu Thu Feb 2 11:28:22 2017 9, 32 -- Received: from catalpa.its.msstate.edu (catalpa.its.msstate.edu [130.18.2.119]) 9, 32 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v12HSMfZ006970 9, 32 -- for {Microscopy-at-microscopy.com} ; Thu, 2 Feb 2017 11:28:22 -0600 9, 32 -- Received: from mail01.ad.msstate.edu (mail01.ad.msstate.edu [130.18.230.60]) 9, 32 -- by catalpa.its.msstate.edu (8.13.8/8.13.8) with ESMTP id v12HY16S019256 9, 32 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=FAIL) 9, 32 -- for {Microscopy-at-microscopy.com} ; Thu, 2 Feb 2017 11:34:01 -0600 9, 32 -- X-Sender: {} 9, 32 -- Received: from MAIL02.ad.msstate.edu (2620:0:1a30:1230::61) by 9, 32 -- mail01.ad.msstate.edu (2620:0:1a30:1230::60) with Microsoft SMTP Server (TLS) 9, 32 -- id 15.0.1236.3; Thu, 2 Feb 2017 11:34:01 -0600 9, 32 -- Received: from MAIL02.ad.msstate.edu ([fe80::7846:3039:9492:24b0]) by 9, 32 -- mail02.ad.msstate.edu ([fe80::7846:3039:9492:24b0%13]) with mapi id 9, 32 -- 15.00.1236.000; Thu, 2 Feb 2017 11:34:01 -0600 9, 32 -- From: "Lawrence, Amanda" {ALawrence-at-i2at.msstate.edu} 9, 32 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 9, 32 -- Subject: M&M 2017 meeting - Student Bursaries 9, 32 -- Thread-Topic: M&M 2017 meeting - Student Bursaries 9, 32 -- Thread-Index: AdJ9eZ4IMkJzdOivRvW3xlKpqOpYMQ== 9, 32 -- Date: Thu, 2 Feb 2017 17:34:00 +0000 9, 32 -- Message-ID: {e24808588a88462983e02454d3ce081e-at-mail02.ad.msstate.edu} 9, 32 -- Accept-Language: en-US 9, 32 -- Content-Language: en-US 9, 32 -- X-MS-Has-Attach: 9, 32 -- X-MS-TNEF-Correlator: 9, 32 -- x-ms-exchange-transport-fromentityheader: Hosted 9, 32 -- x-originating-ip: [130.18.230.93] 9, 32 -- Content-Type: text/plain; charset="us-ascii" 9, 32 -- MIME-Version: 1.0 9, 32 -- Content-Transfer-Encoding: 8bit 9, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v12HSMfZ006970 ==============================End of - Headers==============================
X-from: owen.neill-at-wsu.edu To: Zaluzec-at-microscopy.com
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Email: owen.neill-at-wsu.edu Name: Owen Neill
Organization: Peter Hooper GeoAnalytical Lab, Washington State University
Title-Subject: [Filtered] M&M2017 - Symposium A09
Message: Dear colleagues, Apologies for the cross-postings, but I hope 2017 is off to a good start for everyone. Paper submission for the Microscopy and Microanalysis 2017 Annual Meeting (St. Louis, MO, 6-10 August, 2017) is now open, and will close on 15 February. My co-conveners and I would like to draw your attention to: Symposium A09, Standards, Reference Materials, and Their Applications in Quantitative Microanalysis. We are looking for submissions dealing with the synthesis, evaluation, and need for new reference materials; evaluation, distribution, and maintenance of existing reference materials; the use of standards in quantitative microanalysis; and the application of quantitative microanalytical techniques to solving analytical problems. A full description of this session is included below. Papers may be submitted via the M&M2017 website: http://www.microscopy.org/mandm/2017/ We are also pleased to announce the invited speakers for this symposium: John Hanchar, Department of Earth Sciences, Memorial University, Newfoundland William Nachlas, Department of Earth Sciences, Syracuse University Timothy Rose, Department of Mineral Sciences, Smithsonian Institution Stephen Wilson, Reference Materials Program, United States Geological Survey We are very excited to hear from these experts in microanalytical standards and quantitative microanalysis, and we look forward to hearing about your work as well. See you in St. Louis! The organizers of Symposium A09: Julien Allaz, University of Colorado Boulder Anette von der Handt, University of Minnesota Twin Cities Owen Neill, Washington State University Symposium Description: Standards and reference materials are essential for obtaining accurate quantitative compositional data from X-ray microanalysis by EPMA or SEM (WDS/EDS), as well as from other microanalytical techniques (LA-ICP-MS, SIMS, µ-XRF, FTIR, Raman spectroscopy, etc.). These materials must be rigorously evaluated for their reference compositions and homogeneity, must be widely available to the analytical community, and must be properly maintained to avoid contamination or deterioration. We welcome contributions on the synthesis, evaluation, distribution, and maintenance of standards and reference materials, as well as their appropriate use in microanalysis. We further encourage submissions on standard-based applications of quantitative microanalysis, or on the development of new quantitative microanalytical protocols.
Topics of interest include: The use of standards and reference materials in quantitative microanalysis, and the needs of the analytical community for improving such materials. Synthesis, evaluation, distribution, and maintenance of standards and reference materials. Development of new protocols for microanalytical techniques. Applications of standard-based techniques to solving microanalytical problems.
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Email: jhyun-at-gatan.com Name: John Hyun
Organization: Gatan
Title-Subject: [Filtered] dvanced EELS and EFTEM Training School - France 2017
Message: The Advanced EELS and EFTEM Training School hosted by EDF R&D is an intensive 4-day training school that incorporates lectures, computer laboratories, and microscope practical classes to provide participants with a comprehensive, hands-on training on advanced electron energy loss spectroscopy (EELS) and energy-filtered transmission electron microscopy (EFTEM) topics.
The practical sessions on the microscope will be given at the Materials Ageing Institute. This course focuses on the advanced practice of EELS based imaging and analysis in the (S)TEM microscope on advanced based EELS techniques.
A good experience with electron microscopy and EELS is highly recommended. By the end of the course, participants can expect to get the most out of their EELS spectrometer system and know how to best optimize the experimental conditions in order to capture the maximum amount of information out of the TEM samples using EELS.
Online information and registration: http://www.gatan.com/company/events/advanced-eels-and-eftem-training-school-france-2017
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Email: jwsandinod-at-unal.edu.co Name: John W. Sandino
Organization: Universidad Nacional de Colombia
Title-Subject: [Filtered] Need Gatan 794 Camera Control
Message: Two years ago we received an old 794 retractable camera Gatan as donation from the Tribenberg Lab (closed). But two days ago the camera controller of this camera does not work any more. Today the gatan people confirm the news and told as that the solution would buy a new camera. However we don't have budget for that.
We ask for some who could have a camara contoller that coud give us for free. I know that it is no the best petition but for the moment is the unique solution to work with our tecnai 20. one of the best microscopes in our country Colombia.
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Message: mmc2017 and EMAG 2017 Abstract Submission Deadline - 17 February ---------------------------------------------------
The Microscience Microscopy Congress is returning to Manchester from 3 - 6 July 2017 and now is the time to join us at this great event by submitting an abstract!
www.mmc-series.org.uk/abstracts There is only 2 weeks left to submit an oral abstract as submission closes on Friday 17 February 2017.
The Call for Papers covers a wide variety of topics and techniques as you can see below:
Life Sciences Sessions - Bio Applications: Investigating Biological Structure using Electron Tomography - Imaging in Flow Cytometry - Imaging protein dynamics in living cells - Investigating Biological Structure using Electron Tomography - Bio Applications: Imaging Cells in 3D - matrix, tissue, in vivo - Bio EM Sample Preparation and Analysis - Bio Applications: Imaging Cancer - Correlative Microscopy - Bio-Applications: Long-term imaging using Single Plane Illumination Microscopy - Biological Processes at the Nanoscale (Celtic Session organised by MSI and SMG) - Microbial Imaging - Host-Pathogen Interactions - Bio Applications: Bacterial Ultrastructure - Electron Cryomicroscopy of Biological Macromolecules - Biological Applications of 3D Electron Microscopy Frontiers in Bioimaging Sessions - Bespoke light microscopy for molecular level imaging - Advances in labelling for super-resolution microscopy - Advanced light imaging for addressing longer length scale biological questions - Developing super-resolution methods for functional insight
Physical Sciences Sessions - Earth, Environmental and Planetary Materials - Microscopy of Engineered Surfaces and Tribology - Energy and Energy Storage Materials - Inorganic Nanomaterials (Celtic Session organised by MSI and SMG)
EMAG 2017 Keywords Instrumentation - 3-D microscopy - in-situ microscopy techniques - Low Dose Imaging and Analytical Microscopy - Dynamic TEM - Advances in SEM & FIB (CL, EBSD, beam deceleration, monochromators etc) - Detector technologies and new instrumentation - Vortex Beams and Phase plates - Electron crystallography and diffraction Materials - Biological materials, Biomaterials and Soft matter - Geological microscopy - Catalytic materials - Nanomaterials and 2D materials - Energy and Energy Storage Materials - Functional Materials - Structural Materials and Metallurgy - Surface imaging and modification - Microscopy of interfaces and heterostructures - High spatial resolution chemical and structural analysis
Scanning Probe Microscopy Sessions - Frontiers of Scanning Probe Microscopy - High Resolution SPM - Structures, Interfaces and Mechanics in Life and Health with AFM - SPM nano-mapping of materials properties
Not sure where your work would fit? You can view further details about each conference session at www.mmc-series.org.uk/conference/conference-sessions Submit your abstract at www.mmc-series.org.uk/abstracts Conference Registration is now open, book your place at www.mmc-series.org.uk/registration
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Email: hexi-at-missouri.edu Name: Xiaoqing He
Organization: Universtiy of Missouri
Title-Subject: [Filtered] Oxford INCA EDS detector dead time 100%
Message: Hi all,
We are having issues with our Oxford EDS detector on our F30. With no electron beam on, the dead time stayed at 100% no matter which dispersion rate we choose. Initially we thought the misconnection between Oxford and TEM may be responsible. But the issue persists even after we reboot Microscope PC, Oxford x-stream processor, software. etc.
Any inputs are greatly appreciated!
Thanks.
Xiaoqing He
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One Gatan 794 controller is listed on E*Bay and the seller is willing to ship to Colombia - this is not free, but certainly less expensive then a new camera:
Look really hard for local people who do component level repair of complex electronics - if the problem is in controller and not the camera, then most likely it is repairable! You just need to find someone capable of such repair and willing to do it for the money you are able to pay...
Best Wishes, Valery
Valery Ray www.linkedin.com/in/valeryray ============================== PBS&T, MEO Engineering Company 290 Broadway, Suite 298 Methuen, MA 01844, USA Phone: +1-978-305-0479 - leave a message Mobie: +1-978-305-0479 - leave a message E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 2/3/2017 11:40 PM, microscopy.listserver-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: jwsandinod-at-unal.edu.co } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both jwsandinod-at-unal.edu.co as well as the } Microscopy Listserver } --------------------------------------------------------------------------- } } Email: jwsandinod-at-unal.edu.co Name: John W. Sandino } } Organization: Universidad Nacional de Colombia } } Title-Subject: [Filtered] Need Gatan 794 Camera Control } } Message: Two years ago we received an old 794 retractable camera Gatan } as donation from the Tribenberg Lab (closed). } But two days ago the camera controller of this camera does not work any } more. Today the gatan people confirm the news and told as that the } solution would buy a new camera. However we don't have budget for that. } } We ask for some who could have a camara contoller that coud give us for } free. } I know that it is no the best petition but for the moment is the unique } solution to work with our tecnai 20. one of the best microscopes in our } country Colombia. } } Thanks } Login Host: 168.176.239.31 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } }
==============================Original Headers============================== 7, 35 -- From vray-at-partbeamsystech.com Sat Feb 4 07:32:13 2017 7, 35 -- Received: from nm50-vm4.bullet.mail.bf1.yahoo.com (nm50-vm4.bullet.mail.bf1.yahoo.com [216.109.115.223]) 7, 35 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v14DWDue008165 7, 35 -- for {microscopy-at-microscopy.com} ; Sat, 4 Feb 2017 07:32:13 -0600 7, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1486215479; bh=Kvf3sCTRvNZssax2FMh6A+6/xp5xbJ0jBHlDEyhYwbU=; h=Subject:To:References:Cc:From:Date:In-Reply-To:From:Subject; b=B7tTVfyb8uaGzpNWPWoJ8AW2pnI3rp2wwMUsgRSvnZXDO8wwWhVv022GXD8lc7RGQn0roQv9BPFSkkm6wN3UKIf6LxP+eBM6qNQVh6g92BfGQoTGJmZ1L6T940CeAKBPdBnHaDwsTjptTLVKnNYh8Y7+9rmib6K5iyStTsu0Dviobc0Ry7bzKUGGc/sva2PYMzP4iPP9gP8EIJ0XNE6x99evcHQZxgWonDahTCQaU8tQX59zSxYejh5WnCJke7ffod45XVXljYcOxx69BM3GmCVLVXKqVBpuY2+vqod01ygTqIIl6T452FNKaA3y9ilBO+pUF+sFqqbQK6dnC1kkTQ== 7, 35 -- Received: from [98.139.214.32] by nm50.bullet.mail.bf1.yahoo.com with NNFMP; 04 Feb 2017 13:37:59 -0000 7, 35 -- Received: from [68.142.230.72] by tm15.bullet.mail.bf1.yahoo.com with NNFMP; 04 Feb 2017 13:37:59 -0000 7, 35 -- Received: from [127.0.0.1] by smtp229.mail.bf1.yahoo.com with NNFMP; 04 Feb 2017 13:37:59 -0000 7, 35 -- X-Yahoo-Newman-Id: 328897.38291.bm-at-smtp229.mail.bf1.yahoo.com 7, 35 -- X-Yahoo-Newman-Property: ymail-3 7, 35 -- X-YMail-OSG: Iqa8ro0VM1l3SUOY6n9JFctUwnQfL2FTxNWe8xwurJAkuLv 7, 35 -- 2jClanO7SfQiX2QIMxLHeqev.hI5WQ6Nv4U7YiFyVFKauBSd6ThU0.Prenyu 7, 35 -- WcGwwuSlfJvSPWzwMpFBCl2O9ksFRfrsE0A0Pmf1GL4z6kyXqN0FcMyjCyM7 7, 35 -- hUXwGnGSSxgPRIXq34.IMBSs8Vjmhy8T73yaUOf.8f1f8L20NDzvnjSWjsmP 7, 35 -- gr.R6qsbEiYtR0gw2HksFsZmv9F_ZgyJZ0SvG1sHRirCKMlRr4KgLaaKZmLq 7, 35 -- iIF5r6M9zxGp32bHALADFsyJAHlktplka3CvIqnpXKI.3JiO9NGYrosdjeef 7, 35 -- uaRhb3K0hWBDinO_X0goV_ouFmLFMzrFkDqA2Lr9NTULfsMh3MckiH15Hfwe 7, 35 -- t3YCH6.PHHIBq2o_uVWyj5r.ytassCl1Iv3fyl5JxyqF_AgWBveH3yKtYfiR 7, 35 -- RqcQrAwMBnliZA7zWpnl8ScrJYOUINo9F521dSESZnPcuEjCdw3yvuyDtsQP 7, 35 -- OWjKtKWbpS0TrP_5qUuSs.wVVPIN_qe._xsQRdfMpAdk52kWe8ZVTBkgzm9y 7, 35 -- p6MECMWrS 7, 35 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 7, 35 -- Subject: Re: [Microscopy] viaWWW: Need Gatan 794 Camera Controller 7, 35 -- To: jwsandinod-at-unal.edu.co 7, 35 -- References: {201702040440.v144eAf1022395-at-microscopy.com} 7, 35 -- Cc: microscopy-at-microscopy.com 7, 35 -- From: Valery Ray {vray-at-partbeamsystech.com} 7, 35 -- Message-ID: {209c6b72-69bd-ad5c-047a-60c211cbc756-at-partbeamsystech.com} 7, 35 -- Date: Sat, 4 Feb 2017 08:35:16 -0500 7, 35 -- User-Agent: Mozilla/5.0 (Windows NT 10.0; WOW64; rv:45.0) Gecko/20100101 7, 35 -- Thunderbird/45.7.0 7, 35 -- MIME-Version: 1.0 7, 35 -- In-Reply-To: {201702040440.v144eAf1022395-at-microscopy.com} 7, 35 -- Content-Type: text/plain; charset=windows-1252; format=flowed 7, 35 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Dear All, thank you very much for those who have kindly made some recommendations! I have summarized here the ones that have been recommended
1. Günter Resch (Nexperion, Vienna) 2. TipTemp 3. Extech RH520A temperature/humidity data logger 4. Vernier.com, Go Direct sensors
Hope this will be useful for the rest as well!
Cheers, Yee Yan
On Thursday, 26 January 2017, 16:51, YY YY {rongchigram79-at-yahoo.com.sg} wrote:
Dear All, i would like to ask for your recommendation for a temperature logger. Probably a reasonable sensitivity, acuracy and precision and is able to monitor live using the TEM pc. I am not sure if such system will interfere any performance of the TEM. If you encounter such problem, is it possible to share your experience? I do know some logger has wifi/bluetooth capability, will this interfere the TEM performances well? whiCurrently we have a temperature logger but its not live and we have to extract the data out from time to time which is very troublesome.
Cheers, Yee Yan, Tay FACTS Lab NTU Singapore
==============================Original Headers============================== 14, 31 -- From rongchigram79-at-yahoo.com.sg Sat Feb 4 09:48:05 2017 14, 31 -- Received: from nm4-vm3.bullet.mail.sg3.yahoo.com (nm4-vm3.bullet.mail.sg3.yahoo.com [106.10.148.130]) 14, 31 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v14Fm4qg002152 14, 31 -- for {Microscopy-at-microscopy.com} ; Sat, 4 Feb 2017 09:48:04 -0600 14, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com.sg; s=s2048; t=1486223629; bh=InN6iismgRcLjVRePsrCmyRWCaq6BX9xZsC/YqvrBzU=; h=Date:From:Reply-To:To:In-Reply-To:References:Subject:From:Subject; b=pPoSUpQM1id9HQwuYggD8s/U7Lpn6ndKZmmXExAaMrxHV7tR9adwsmSzfdfq7wgy5LMQG3P5jbtYFCZFg37TTa0V7Ga+D5E91KAweOp8VxJje2hsWZhNCiMg0rEBqnJ/KTCFbKP04kiLSPEs3JA+7dfLOkah0NagkVAEDcSetxnUcrcGnHX9xkxAvFGgKRM2/KamonrvJ7+zNG9jo5fHxoIkeTt7ymMM4eIMN7bTyp8MJMix0x3cnt/AV6Sqg8gEDUGoFeravmGHi4RLz9Vyi5rRk5vElE1FCDRZcNrMPh6nFx6kegrEhEmJet+00F2F4xnZfXjpTzvNDrfuvUcYhw== 14, 31 -- Received: from [106.10.166.126] by nm4.bullet.mail.sg3.yahoo.com with NNFMP; 04 Feb 2017 15:53:49 -0000 14, 31 -- Received: from [106.10.151.235] by tm15.bullet.mail.sg3.yahoo.com with NNFMP; 04 Feb 2017 15:53:49 -0000 14, 31 -- Received: from [127.0.0.1] by omp1019.mail.sg3.yahoo.com with NNFMP; 04 Feb 2017 15:53:49 -0000 14, 31 -- X-Yahoo-Newman-Property: ymail-3 14, 31 -- X-Yahoo-Newman-Id: 815924.4478.bm-at-omp1019.mail.sg3.yahoo.com 14, 31 -- X-YMail-OSG: xxd7uisVM1lP6tiAU0DMME8W61Dbov08AcQINWFu5CGiA_L2lw8OYEExyWl4_Sc 14, 31 -- edyDDkVe4oA6ZTVIG4CVrgBjmqMBFn_2x4EpZgRhBxPHERRqHcaXNa7HkAkdjqUvOD9sAdqNnaXB 14, 31 -- 1y48At6xvZYOlxrM.ojGxuQeCK.lciAZaflk8uhs0a0Uwgj2DRh5Iuoyw0bQ5zna6kaCs_qp0Hsp 14, 31 -- s.ddGYpr0SKxpbHUY4RlZv2GhHxPAbs9xTcp5IByEtLI3VQ.jv8OMqyxGfvca8dx8Xrqegxa6pVm 14, 31 -- tzsMJ6N4ioOXMekldlVPAE2RdalI2xTvFxQLtjHvfXLnU5a9ayjZL8XwK0TEu.ZmHT1ZpWUwQpGc 14, 31 -- gAbEfZ1j4.QL4AKqavK3pq4712puLeWzSXLdEPfknOohmlimO8ihEDnTXgpbgEbWtI._NmdAvNj7 14, 31 -- ndpXnPI1NvhpBXzyPqiNjQC2HF9lkp.V7QZwYFVI_k7ERqX.U9z2KthKfPj1QXXvVSK9PO9oGB2k 14, 31 -- MUFKWjGyccmb6D7SFmgDQzKJlXVhv_ePh8LMRpzqq0J2BiBimrBY- 14, 31 -- Received: from jws600005.mail.sg3.yahoo.com by sendmailws116.mail.sg3.yahoo.com; Sat, 04 Feb 2017 15:53:49 +0000; 1486223629.458 14, 31 -- Date: Sat, 4 Feb 2017 15:53:48 +0000 (UTC) 14, 31 -- From: YY YY {rongchigram79-at-yahoo.com.sg} 14, 31 -- Reply-To: YY YY {rongchigram79-at-yahoo.com.sg} 14, 31 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 14, 31 -- Message-ID: {1376590105.1143844.1486223628989-at-mail.yahoo.com} 14, 31 -- In-Reply-To: {1775741231.1160911.1485420702386-at-mail.yahoo.com} 14, 31 -- References: {1775741231.1160911.1485420702386.ref-at-mail.yahoo.com} {1775741231.1160911.1485420702386-at-mail.yahoo.com} 14, 31 -- Subject: Re: Temperature Logger to Recommend 14, 31 -- MIME-Version: 1.0 14, 31 -- Content-Type: text/plain; charset=UTF-8 14, 31 -- Content-Transfer-Encoding: 8bit 14, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v14Fm4qg002152 ==============================End of - Headers==============================
From stepnoah502-at-gmail.com Sun Feb 5 10:47:09 2017 Return-Path: {stepnoah502-at-gmail.com} Received: from gmail.com ([61.76.233.68]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v15Gl6IX001296 for {microscopylistserverarchive6-at-microscopy.com} ; Sun, 5 Feb 2017 10:47:08 -0600 Message-ID: {F404FFFA.5BD98938-at-gmail.com}
http://www.practicaldesign.com/THUM/thum.html
I have used two of these for over a decade to monitor T/H in microscope rooms. USB interface, so PC should be on, and data stored in a database.
Cheers,
Fred Monson
-----Original Message----- X-from: rongchigram79-at-yahoo.com.sg [mailto:rongchigram79-at-yahoo.com.sg] Sent: Saturday, February 04, 2017 10:59 AM To: Monson, Frederick {FMonson-at-wcupa.edu}
Dear All, thank you very much for those who have kindly made some recommendations! I have summarized here the ones that have been recommended
1. Günter Resch (Nexperion, Vienna) 2. TipTemp 3. Extech RH520A temperature/humidity data logger 4. Vernier.com, Go Direct sensors
Hope this will be useful for the rest as well!
Cheers, Yee Yan
On Thursday, 26 January 2017, 16:51, YY YY {rongchigram79-at-yahoo.com.sg} wrote:
Dear All, i would like to ask for your recommendation for a temperature logger. Probably a reasonable sensitivity, acuracy and precision and is able to monitor live using the TEM pc. I am not sure if such system will interfere any performance of the TEM. If you encounter such problem, is it possible to share your experience? I do know some logger has wifi/bluetooth capability, will this interfere the TEM performances well? whiCurrently we have a temperature logger but its not live and we have to extract the data out from time to time which is very troublesome.
Cheers, Yee Yan, Tay FACTS Lab NTU Singapore
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==============================Original Headers============================== 24, 41 -- From FMonson-at-wcupa.edu Mon Feb 6 14:57:45 2017 24, 41 -- Received: from MX01.WCUPA.EDU (mx01.wcupa.edu [144.26.63.2]) 24, 41 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v16KvjIF017282 24, 41 -- for {microscopy-at-microscopy.com} ; Mon, 6 Feb 2017 14:57:45 -0600 24, 41 -- X-ASG-Debug-ID: 1486415019-08911c2a59823d60001-4CH8be 24, 41 -- Received: from WCUXCHP02.PASSHE.LCL ([144.26.2.138]) by MX01.WCUPA.EDU with ESMTP id HXluSUbrlLVvkHGs (version=TLSv1.2 cipher=ECDHE-RSA-AES256-SHA384 bits=256 verify=NO); Mon, 06 Feb 2017 16:03:39 -0500 (EST) 24, 41 -- X-Barracuda-Envelope-From: FMonson-at-wcupa.edu 24, 41 -- X-Barracuda-Apparent-Source-IP: 144.26.2.138 24, 41 -- X-ASG-Whitelist: Client 24, 41 -- Received: from WCUXCHP08.PASSHE.LCL (144.26.2.144) by WCUXCHP02.PASSHE.LCL 24, 41 -- (144.26.2.138) with Microsoft SMTP Server (TLS) id 15.0.1178.4; Mon, 6 Feb 24, 41 -- 2017 16:02:56 -0500 24, 41 -- Received: from WCUXCHP08.PASSHE.LCL ([144.26.2.144]) by WCUXCHP08.PASSHE.LCL 24, 41 -- ([144.26.2.144]) with mapi id 15.00.1178.000; Mon, 6 Feb 2017 16:02:56 -0500 24, 41 -- From: "Monson, Frederick" {FMonson-at-wcupa.edu} 24, 41 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 24, 41 -- CC: "rongchigram79-at-yahoo.com.sg" {rongchigram79-at-yahoo.com.sg} 24, 41 -- Subject: RE: [Microscopy] Re: Temperature Logger to Recommend 24, 41 -- Thread-Topic: [Microscopy] Re: Temperature Logger to Recommend 24, 41 -- X-ASG-Orig-Subj: RE: [Microscopy] Re: Temperature Logger to Recommend 24, 41 -- Thread-Index: AQHSfwBcAS2bYFzvIUe8JTzF+vus06FceloQ 24, 41 -- Date: Mon, 6 Feb 2017 21:02:56 +0000 24, 41 -- Message-ID: {9cec4e3d33244e649868e83a17bbf094-at-WCUXCHP08.PASSHE.LCL} 24, 41 -- References: {201702041559.v14FxIjV012791-at-microscopy.com} 24, 41 -- In-Reply-To: {201702041559.v14FxIjV012791-at-microscopy.com} 24, 41 -- Accept-Language: en-US 24, 41 -- Content-Language: en-US 24, 41 -- X-MS-Has-Attach: 24, 41 -- X-MS-TNEF-Correlator: 24, 41 -- x-ms-exchange-transport-fromentityheader: Hosted 24, 41 -- x-originating-ip: [144.26.2.58] 24, 41 -- Content-Type: text/plain; charset="iso-8859-1" 24, 41 -- MIME-Version: 1.0 24, 41 -- X-Barracuda-Connect: UNKNOWN[144.26.2.138] 24, 41 -- X-Barracuda-Start-Time: 1486415019 24, 41 -- X-Barracuda-Encrypted: ECDHE-RSA-AES256-SHA384 24, 41 -- X-Barracuda-URL: https://SPAMCONTROL.WCUPA.EDU:443/cgi-mod/mark.cgi 24, 41 -- X-Virus-Scanned: by bsmtpd at WCUPA.EDU 24, 41 -- X-Barracuda-BRTS-Status: 1 24, 41 -- Content-Transfer-Encoding: 8bit 24, 41 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v16KvjIF017282 ==============================End of - Headers==============================
From marilore504-at-gmail.com Mon Feb 6 18:14:02 2017 Return-Path: {marilore504-at-gmail.com} Received: from gmail.com ([112.121.188.90]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v170DxpG016138 for {microscopylistserverarchive6-at-microscopy.com} ; Mon, 6 Feb 2017 18:14:01 -0600 Message-ID: {9DCA92EA.3D42EAF7-at-gmail.com}
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From ronaldfaust205-at-gmail.com Wed Feb 8 06:27:11 2017 Return-Path: {ronaldfaust205-at-gmail.com} Received: from gmail.com ([218.232.94.109]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v18CR8Eb016233 for {microscopylistserverarchive6-at-microscopy.com} ; Wed, 8 Feb 2017 06:27:10 -0600 Message-ID: {CA32B257.17A4C6A8-at-gmail.com}
Dear Listers,
I am wondering if anyone may have installation/service manual for Jeol JEM 2100F TEM, or any notes related to its installation you would be willing to share. I have full operation manual with the instrument, but need to move it.
Thank you very much beforehand, -- Valery Ray www.linkedin.com/in/valeryray ============================== PBS&T, MEO Engineering Company 290 Broadway, Suite 298 Methuen, MA 01844, USA Phone: +1-978-305-0479 - leave a message Mobie: +1-978-305-0479 - leave a message E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
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Message: Position: Technical Sales Representative - Mid Atlantic US Territory: Dependent on the candidate but will include MD, DE, DC, VA, NJ & PA. Products: Sales and support of the following products/companies: Hitachi: Bench-top Scanning Electron Microscopes (SEM) and AFM Kleindiek: Nano-manipulators, Probe Stations and Stages Leica: Electron Microscopy sample prep. equipment EMSIS TEM Cameras Deben Tensile Stages and Microscope AccessoriesJ V/Bruker Semi: X-ray Diffraction (XRD)
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Hello, It could be that you have different grounding points in use, and an electric potential between them causing a ground current being the issue. You might want to check that your ground lines are correctly wired.
The general recommendation is to use the frame of the TEM as the only grounding point for your TEM connected accessories, arranging a grounding bus at the frame and avoiding any ground loops between the accessories. For instance, do not use the house grounding line feeding to the power socket which powers the EDX equipment and EDX computer. The house grounding line stays disconnected from this power socket. This socket's grounding (not the house grounding line) becomes linked to the frame of the TEM, instead. If I remember correctly, then Oxford ships an accordingly manipulated distributor with their equipment for easing such wiring. Don't forget to also connect the chassis of the EDX computer to your grounding point at the frame of the TEM. Consult a certified electrician for assuring electrics security in your setup!
Best greetings from the EM-Labs of CIC biomaGUNE (Spain), Marco Mller
} } Email: hexi-at-missouri.edu Name: Xiaoqing He } } Organization: Universtiy of Missouri } } Title-Subject: [Filtered] Oxford INCA EDS detector dead time 100% } } Message: Hi all, } } We are having issues with our Oxford EDS detector on our F30. With no } electron beam on, the dead time stayed at 100% no matter which } dispersion rate we choose. Initially we thought the misconnection } between Oxford and TEM may be responsible. But the issue persists even } after we reboot Microscope PC, Oxford x-stream processor, software. } etc. } } Any inputs are greatly appreciated! } } Thanks. } } Xiaoqing He
==============================Original Headers============================== 6, 46 -- From mmoller-at-cicbiomagune.es Thu Feb 9 02:21:04 2017 6, 46 -- Received: from webmail.cicbiomagune.es (webmail.cicbiomagune.es [150.241.252.34]) 6, 46 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v198L3Dr031690 6, 46 -- for {Microscopy-at-microscopy.com} ; Thu, 9 Feb 2017 02:21:04 -0600 6, 46 -- X-AuditID: 0a0d0165-ff5ff70000007f79-8f-589c27d7c066 6, 46 -- Received: from cicmgex1.cicbiomagune.int (cicmgex1.cicbiomagune.int [10.10.1.98]) 6, 46 -- by webmail.cicbiomagune.es (Symantec Messaging Gateway) with SMTP id 34.E1.32633.7D72C985; Thu, 9 Feb 2017 09:27:05 +0100 (CET) 6, 46 -- Received: from cicmgex1.cicbiomagune.int (10.10.1.98) by 6, 46 -- cicmgex1.cicbiomagune.int (10.10.1.98) with Microsoft SMTP Server (TLS) id 6, 46 -- 15.0.1210.3; Thu, 9 Feb 2017 09:27:03 +0100 6, 46 -- Received: from CICMGEXCAS.cicbiomagune.int (10.10.1.3) by 6, 46 -- cicmgex1.cicbiomagune.int (10.10.1.98) with Microsoft SMTP Server (TLS) id 6, 46 -- 15.0.1210.3 via Frontend Transport; Thu, 9 Feb 2017 09:27:02 +0100 6, 46 -- Received: from CICMGEXMBX.cicbiomagune.int ([::1]) by 6, 46 -- CICMGEXCAS.cicbiomagune.int ([::1]) with mapi id 14.03.0195.001; Thu, 9 Feb 6, 46 -- 2017 09:27:02 +0100 6, 46 -- From: Marco Moller {mmoller-at-cicbiomagune.es} 6, 46 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} , 6, 46 -- "hexi-at-missouri.edu" {hexi-at-missouri.edu} 6, 46 -- Subject: RE: Oxford INCA EDS detector dead time 100% 6, 46 -- Thread-Topic: Oxford INCA EDS detector dead time 100% 6, 46 -- Thread-Index: AQHSgq5J87TxHrm1zUCxCnJ2dAce0A== 6, 46 -- Date: Thu, 9 Feb 2017 08:27:02 +0000 6, 46 -- Message-ID: {216351DE18DE144BBBFC1996E5FAEBB204E9F2-at-CICMGEXMBX.cicbiomagune.int} 6, 46 -- References: {201702040502.v1452nBu002756-at-microscopy.com} 6, 46 -- Accept-Language: de-DE, es-ES, en-US 6, 46 -- Content-Language: en-US 6, 46 -- X-MS-Has-Attach: 6, 46 -- X-MS-TNEF-Correlator: 6, 46 -- x-originating-ip: [10.10.2.151] 6, 46 -- Content-Type: text/plain; charset="iso-8859-1" 6, 46 -- MIME-Version: 1.0 6, 46 -- X-Brightmail-Tracker: H4sIAAAAAAAAA+NgFuplkeLIzCtJLcpLzFFi42Lh4mJM0r2pPifCYOsPPYvL5+8zWvw53MDu 6, 46 -- wOTxsfs1k0fPzOAApigum5TUnMyy1CJ9uwSujLvnb7MXrOSr+LboCFsD42nuLkZODgkBE4n3 6, 46 -- jy6ydTFycQgJXGGUOHZjIzNIQkhgM6NE09kiiMRpRokNJ86zQDgzGSUa185iAaliE9CReHT6 6, 46 -- OpgtIpAmMen5XLBuYaCxP+7MZ4WIm0o0rZ7DBGHrSdx7vo4NxGYRUJG48H06WC+vQIDEqcbt 6, 46 -- TBCbLSW6H0wBsjk4GIFqNrzSBQkzC4hL3HoynwniagGJJXvOM0PYohIvH/9jBSmXEJCXuDXN 6, 46 -- EaJcT+LG1ClsELa2xLKFr5khNglKnJz5hAVik4rE7jcr2Ccwis1CsmEWkvZZSNpnIWlfwMiy 6, 46 -- ilE6qSgzPaMkNzEzRy85MzkpMz83Mb00L1UPGFGbGEGRxMuYuoNx/0TdQ4wCHIxKPLwVlrMj 6, 46 -- hFgTy4orcw8xSnAwK4nwFirPiRDiTUmsrEotyo8vKs1JLT7EKM3BoiTOm+VTFyEkkJ5Ykpqd 6, 46 -- mlqQWgSTZeLglGpgZA56/0zyrEQK95Xt/Wcv8apsZuy49NUgMij0hdipNzOjE6p/ZeXwzGB6 6, 46 -- FpR++fWrVwU7nn268mhFya2sp241H0qTwnlXuipcZdCcVTTp8ssW8w0nYoT79ly8GbSiWHlK 6, 46 -- WXtxtaxSj33VCuvPdfu4mlLTVmrp7d4ddXWD4H8W+5VXpaurQmxmKrEUZyQaajEXFScCAJj+ 6, 46 -- vRagAgAA 6, 46 -- Content-Transfer-Encoding: 8bit 6, 46 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v198L3Dr031690 ==============================End of - Headers==============================
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Email: thomas.horn-at-bsse.ethz.ch Name: Thomas Horn
Organization: ETH Zurich
Title-Subject: [Filtered] Position open in Basel, Switzerland: Microscopy Engineer/Microscopist
Message: Dear all, we have an open position at the Single Cell Facility , ETH, Department of Biosystems Science and Engineering (D-BSSE), Basel: Microscopy Engineer / Microscopist (m/f) Responsibilities are training and support of facility users. This includes microscopy instrument quality control and their maintenance. The microscopy engineer/microscopist will develop and implement customized configurations and the setup of the experimental workflows of experiments on the automated imaging systems. The successful candidate will share responsibilities with our other microscopy expert and work closely together with our software engineer for image analysis and data management. This position is available for 2 years and located in Basel. Requirements are a degree from a technical college or a university with a proven experience in various advanced light microscopy and digital imaging technologies. She/he will have extensive technical experience with state-of-the-art light microscopy platforms such as confocal, live-cell imaging and/or SPIM. Strong knowledge of advanced quantitative imaging technologies as well as their underlying optical principles is required. Scripting experience is a plus. The candidate should have hands-on skills in microscopy component integration. This position demands very good communication skills in English to interact with scientists and students, and the skill to adapt to the service character of the facility to successfully support scientific research and operations at the D-BSSE. Besides having excellent technical abilities in the field of light microscopy, the candidate must be an excellent team player and possess the ability to work in a variety of scientific areas. The Single Cell Facility (SCF) is a central scientific core facility within the ETH Zurich, Department of Biosystems Science and Engineering (D-BSSE), located in Basel. The SCF serves the department by providing technology and support in advanced (light-sheet, automated wide field, confocal) microscopy and flow cytometry. For further information about the position, please contact Dr. Thomas Horn, by email: thomas.horn-at-bsse.ethz.ch (no applications).
Applications are only accepted online, please use the Apply now button at the link below. Please address it to: ETH Zurich, Roland Munz, Human Resources, CH-8092 Zurich.
Further information on our facility: https://www.bsse.ethz.ch/scf
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Email: jhyun-at-gatan.com Name: John Hyun
Organization: Gatan
Title-Subject: [Filtered] EELS Training School - April 2017, California
Message: EELS & EFTEM Analysis Training School
April 4-7, 2017, Gatan R&D Headquarters, Pleasanton , CA
Electron energy loss spectroscopy (EELS) is a powerful technique that provides both compositional and chemical information from sub-nanometer areas in the sample. As a course attendee, you will learn best practices to set up and optimize your EELS hardware and experimental protocols so you can capture and extract the maximum amount of compositional and chemical information from your TEM samples. Topics include:
Fundamentals of EELS and energy-filtered imaging in TEM Principles of operation of Gatan EFTEM and EELS systems Optimization of EFTEM and EELS data acquisition Quantification of elemental composition Other information provided by EFTEM/EELS and how best to extract it Use of EELS signals to form maps of elemental and chemical composition EFTEM and STEM EELS spectrum imaging techniques Identification of material phases via EELS fine structure mapping Applications to biological and physical science specimens
School details and online registration: http://www.gatan.com/company/events/eels-eftem-analysis-training-school-april-2017
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From shkrsugi29-at-gmail.com Sun Feb 12 11:00:56 2017 Return-Path: {shkrsugi29-at-gmail.com} Received: from gmail.com ([112.121.167.82]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v1CH0r1q017848 for {microscopylistserverarchive6-at-microscopy.com} ; Sun, 12 Feb 2017 11:00:55 -0600 Message-ID: {27532BAF.4AEABBE4-at-gmail.com}
Hi,
We have an old Gauss Maus (Dindima Group/Arlunya) , a hand held device for measuring magnetic fields. We need to work out the magnitude and direction of some stray fields that have just appeared (intermittently of course) in our lab but unfortunately have lost the manual. I have searched on the internet but couldn't find a manual there. If anyone has a copy of the manual they could scan and email to me or, send to me so that I can scan it and send it back it would be much appreciated.
E colin.veitch-at-csiro.au T +61 3 5246 4891 M 0438 538 475 F +61 3 5246 4057 Address CSIRO Manufacturing, 75 Pigdons Road, Waurn Ponds, Vic 3216, Australia. www.csiro.au | http://www.csiro.au/Organisation-Structure/Flagships/Manufacturing.aspx
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E colin.veitch-at-csiro.au T +61 3 5246 4891 M 0438 538 475 F +61 3 5246 4057 Address CSIRO Manufacturing, 75 Pigdons Road, Waurn Ponds, Vic 3216, Australia. www.csiro.au | http://www.csiro.au/Organisation-Structure/Flagships/Manufacturing.aspx
PLEASE NOTE The information contained in this email may be confidential or privileged. Any unauthorised use or disclosure is prohibited. If you have received this email in error, please delete it immediately and notify the sender by return email. Thank you. To the extent permitted by law, CSIRO does not represent, warrant and/or guarantee that the integrity of this communication has been maintained or that the communication is free of errors, virus, interception or interference.
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Last spring, a thread was started on the quality/longevity of newer LaB6 cathodes. I looked back through the e-mails and it seemed like the last thing that was mentioned was something about ongoing discussions with the vendors.
I have some grant money left that I need to spend soon and was thinking of switching our CM100 over from W to LaB6. I worry, however, that it may not be worth it since it seemed like others were having problems with the longevity of the cathodes.
I did contact the original person who started the thread on this listserv but have yet to receive a reply. Does anyone know what the status of the cathodes is?
Thank you, -Blanca ----------------------------------------- Blanca Carbajal Gonzalez, M.S. Director of Microscopy Science Center 50 College St Mount Holyoke College Clapp Laboratory 123 Office: 413-538-3118 Cell: 559-905-7138 bicarbaj-at-mtholyoke.edu MHC Microscopy
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Email: fengxia.liang-at-med.nyu.edu Name: Alice Liang
Organization: New York University Langone Medical Center
Title-Subject: [Filtered] CryoEM manager-NYU
Message: Cryo EM Manager Position at NYU Langone School of Medicine
NYU Langone School of Medicine is seeking a cryo-EM manager to oversee the operation of our expanding, state-of-the- art electron microscopy facility. The facility will include Titan Krios and Talos Arctica microscopes, both with direct detectors, for single particle EM and cryo electron tomography. The facility will be staffed by 3 team members, including the manager (this position) and two junior associates. The facility manager will be responsible for oversight and maintenance of these microscopes as well as training and assisting users, who will be primarily from the NYU research community. The manager will interface with our High Performance Computing team to ensure a seamless interface between data collection and data processing. The manager will also have the opportunity to, and will be encouraged to interact with cryo EM experts in the New York area and to attend key EM conferences (e.g. EM Gordon Conference) in order to integrate NYU with the greater EM community, and to implement cutting-edge techniques and technologies into our facility.
Responsibilities (together with 2 other team members): Maintain Talos Arctica and Krios microscopes. This duty requires interfacing with FEI engineers to ensure optimal performance of the microscopes. Maintain ancillary, cryo-EM related equipment such as Vitrobot, glow discharger etc. Load samples and align microscopes prior to data collection Train and oversee users for data collection Evaluate quality of data obtained by users Interface and collaborate with High Performance Computing team, who will assist with computational needs for optimal data collection, storage and data processing Interface and collaborate with Microscopy Core, which houses electron microscopes for preliminary screening of samples Implement data collection strategies that will benefit our user community Maintain accurate logs for microscope performance and usage
Key element of the job: This facility will be optimized for high throughput data collection. Developing a pipeline that fits well with our users will be a key part of this job. Although the manager is expected to consult with faculty supervisors, he/she will be given flexibility and responsibility to develop a pipeline that generates high quality data in an efficient manner.
Requirements: PhD in structural biology, biochemistry or related field At least 2 years of cryo EM experience, with expertise in handling samples, using microscopes and evaluating images Excellent communication and interpersonal skills Knowledge of the principles of transmission electron microscopy General understanding of single particle cryo EM workflow; experience in cryo electron tomography is a plus Special consideration will be given to candidates with management experience or a record of achievement in collaborative, team-driven environments
The Ideal Candidate: In addition to meeting all the requirements above, the ideal candidate will also bring enthusiasm and passion to developing our new facility. We are looking for a colleague who will be excited about interacting with researchers at all levels and will consistently strive to maintain a state-of-the-art facility. The EM manager will be an key component of our new cryo EM initiative, and as such, will play a crucial role in shaping the new facility. This job is an excellent opportunity for an electron microscopist who is interested in a leadership role within the vibrant research community at NYU School of Medicine.
Salary will be competitive and job title will depend on qualifications of the candidate.
To apply for this position, please email cryoemnyu-at-gmail.com and include: 1) Cover letter 2) CV 3) Contact information for 3 references
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From stepnoah502-at-gmail.com Wed Feb 15 13:56:35 2017 Return-Path: {stepnoah502-at-gmail.com} Received: from gmail.com (static.vdc.vn [113.160.108.75] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v1FJuWcu027444 for {microscopylistserverarchive6-at-microscopy.com} ; Wed, 15 Feb 2017 13:56:34 -0600 Message-ID: {FB6D4791.5BCEEE94-at-gmail.com}
We need to remove the top of our Leica Ultracut S ultramicrotome to check something on the electrical circuit for the overhead light source. I am talking about the top plate that carries the binocular microscope that allows you to view the specimen and cutting action.
It looks like there is a screw at the very back of this plate that can be seen from the underside when the plate is rotated 90 degrees. I am guessing that one removes that screw and then you can used the normal hand driven gear drive to move the carrier forward until it is free of the underlying dovetail. Can anyone confirm this. Additionally, where does one disconnect the electrical to the fluorescent light source? Will I see a plug once I remove this screw and move the carrier forward?
Thanks, Tom
Thomas E. Phillips, Ph.D Curator's Distinguished Teaching Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
Thomas E. Phillips, Ph.D Curator's Distinguished Teaching Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
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Email: roger.ristau-at-uconn.edu Name: Roger Ristau
Organization: University of Connecticut
Title-Subject: [Filtered] FIB STEM EDX
Message: A question for anyone with FIB-STEM-EDX system: Our FEI Helios 460F1 has single-sample Flip Stage (the 'turret' type Flip Stage with 180 deg rotation) and STEM detector for imaging TEM liftouts in situ. When attempting to acquire EDX of the lifout, mounted on the Flip Stage, using EDAX Octane Plus SDD EDX, the spectrum is overwhelmed by the Si peak from the STEM detector itself. If the STEM detector is retracted, the Si peak is replaced by a Al peak from the stage area situated below the STEM position.
Obviously the EDX detector is 'seeing' the X-rays generated by the e-beam that passes through the thin liftout sample. However, this problem did not occur in our older Strata FIB with SiLi detector. I am presuming it may have something to do with the larger collection angle of the SDD. I have confirmed with EDAX that the detector is installed correctly,the collimator is in position and the system is operating normally. (There are no problems doing EDX with the sample in the 'bulk stage' position.)
My temporary solution is to affix a strip of carbon tape to the 'underside' of the Flip Stage to absorb the transmitted e-beam, eliminating the spurious Al peak, but this is obviously undesirable as that makes STEM imaging impossible.
Has anyone else encountered this with a similar system?
Cheers Roger Ristau Univ of Connecticut
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Message: Hi Listeners, I am using Tecnai TEM equiped with Oxford EDS. Whenever I open the shutter to start the EDS analysis, it's not getting open, it shows overload, while it was not even opened for the days. Could you guys help me to fix this issue.
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Email: steve.sem-at-icloud.com Name: Steve Perry
Title-Subject: [Filtered] Service Engineer for FEI Tecnai
Message: I would like to hear from a service engineer who is capable and available to perform service work on our Tecnai F30.
Located on the West coast USA. Travel and accommodations can be provided if required.
Reply in confidence,
Steve Perry
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For a quick fix - get Beryllium foil of adequate thickness (0.5mm is widely available) and cover areas *behind* the retractable STEM detector exposed to e-beam transmitted through the sample. You could get STEM image with extended detector and EDS + Se image when detector is retracted.
It is possible that once main Al peak is dealt with, some additional stray signal generated by electrons scattered from/through the sample could become detectable - find out where they come from and shield with Be foil.
Be is considered "hazmat," but that is when it is in dust/powder/fume/solution form which can be ingested, inhaled, or absorbed into the body. If you do not drill/sand/machine Be foil but only attach it by conductive vacuum epoxy it should be safe enough to stay there. Lots of Be windows were (and still are) out there installed in SEMs.
If Beryllium is too much of a scare, use Aquadag paint of adequate thickness to cover area behind STEM detector. Best would be to manufacture a shield from some kind of metal, coat it with Aquadag, anneal in vacuum oven -at- 300 degrees, and then mount into SEM chamber.
Best Wishes :) Valery
Valery Ray www.linkedin.com/in/valeryray ============================== PBS&T, MEO Engineering Company 290 Broadway, Suite 298 Methuen, MA 01844, USA Phone: +1-978-305-0479 - leave a message Mobie: +1-978-305-0479 - leave a message E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 2/18/2017 12:44 PM, microscopy.listserver-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: roger.ristau-at-uconn.edu } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } roger.ristau-at-uconn.edu as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: roger.ristau-at-uconn.edu Name: Roger Ristau } } Organization: University of Connecticut } } Title-Subject: [Filtered] FIB STEM EDX } } Message: A question for anyone with FIB-STEM-EDX system: } Our FEI Helios 460F1 has single-sample Flip Stage (the 'turret' type Flip Stage with 180 deg } rotation) and STEM detector for imaging TEM liftouts in situ. When attempting to acquire EDX of the } lifout, mounted on the Flip Stage, using EDAX Octane Plus SDD EDX, the spectrum is overwhelmed by } the Si peak from the STEM detector itself. If the STEM detector is retracted, the Si peak is } replaced by a Al peak from the stage area situated below the STEM position. } } Obviously the EDX detector is 'seeing' the X-rays generated by the e-beam that passes through the } thin liftout sample. However, this problem did not occur in our older Strata FIB with SiLi detector. } I am presuming it may have something to do with the larger collection angle of the SDD. I have } confirmed with EDAX that the detector is installed correctly,the collimator is in position and the } system is operating normally. (There are no problems doing EDX with the sample in the 'bulk stage' } position.) } } My temporary solution is to affix a strip of carbon tape to the 'underside' of the Flip Stage to } absorb the transmitted e-beam, eliminating the spurious Al peak, but this is obviously undesirable } as that makes STEM imaging impossible. } } Has anyone else encountered this with a similar system? } } Cheers } Roger Ristau } Univ of Connecticut } } Login Host: 137.99.20.153 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } }
==============================Original Headers============================== 8, 35 -- From vray-at-partbeamsystech.com Sat Feb 18 13:14:23 2017 8, 35 -- Received: from nm4.bullet.mail.bf1.yahoo.com (nm4.bullet.mail.bf1.yahoo.com [98.139.212.163]) 8, 35 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v1IJENwS001966 8, 35 -- for {microscopy-at-microscopy.com} ; Sat, 18 Feb 2017 13:14:23 -0600 8, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1487445661; bh=1fe6VgbnnVmF55q7McMXYbAnmrIRKYsWcA7y6GWh7+4=; h=Subject:To:References:Cc:From:Date:In-Reply-To:From:Subject; b=uGMeLUE/3KBwbo80hYRR3qU6HJ08YcrUsFREGYXHtJi2HN9cJ/pClbYk9Ye7bDN3QKWoaD5GVytrUtyVcIsVquKnFN7+m18DpgwXvk70Ecen0srVpLo5IT8Ah5OeAZn832Hf2MZBHP/+FZUuO1xk8nbz6gpQc1kJ2iMzf4lz6BUYdNSlsgfGE4BaEeJSOtU/INVMIMWW/DH+lZs0Ia5NCrPs3xQhD0Df2O+VnjIwKZn98b7srRPYL40yuyhafnZpTuU5aZkz4USHmCt2T5TP81lVDmp5t+OpspSLJ0EjMv2IhXbU5DW+oVl9SbrQDUohnSNHp0kgtLz4xeO5ILi/iw== 8, 35 -- Received: from [66.196.81.172] by nm4.bullet.mail.bf1.yahoo.com with NNFMP; 18 Feb 2017 19:21:01 -0000 8, 35 -- Received: from [98.139.211.196] by tm18.bullet.mail.bf1.yahoo.com with NNFMP; 18 Feb 2017 19:21:01 -0000 8, 35 -- Received: from [127.0.0.1] by smtp205.mail.bf1.yahoo.com with NNFMP; 18 Feb 2017 19:21:01 -0000 8, 35 -- X-Yahoo-Newman-Id: 605872.93412.bm-at-smtp205.mail.bf1.yahoo.com 8, 35 -- X-Yahoo-Newman-Property: ymail-3 8, 35 -- X-YMail-OSG: mMcXsfoVM1lZqQV.3CFdk2J.BBWfj1b7tSwuEim4SB.HUwn 8, 35 -- qMBRntpiQ.i4QVKnB_rl_kWQoIFz3ixhsNfMXQ_Hb7JPPbnUcqBoWtxMPQ9A 8, 35 -- _jCDMphgfujnjq8r6XzQA8nXC_PnX2QylJZQjzBJaosCkYytJ0KSB2rc2Pel 8, 35 -- aASk5yVWBbI_rnaGJvrRoMHftzv0dWwXNp9sTo3n4JB.9QLtKC2vZxfVEqNY 8, 35 -- 3rpMKaphhkhTCofLwsYMzrhU.Bujbbh2ztk7ZE6qUcVwuB95_7d81pPrTZJw 8, 35 -- kaHZMJ4BK.X4s6JCjs1RlBbFGT.ta_.8FO8DTxoPBwMg8JQL9cABp1PMEMqx 8, 35 -- UAvBu9pjseMYVvNWMXSZ1ziEFp.hBxkMySqQTfmV7CcaiI.H1HIttdbaRdiS 8, 35 -- 5s0036JhDKpJZHkIte9dsJe_tuia8jmjWmVpBZ4a.LAFKjIvnpPAkc7Y5ntA 8, 35 -- w.ehvEY1jAHS5VB4CVKI8B8gky0uQ1Rs5UL9zK.QFKSUD.kXPb043uX0WtJC 8, 35 -- VlfQvt61_J7IaEmf.upn2m.flVDYhRjzPnGcWNcEtkOuV2rtg.9gWw7xVMVT 8, 35 -- OywvmZY_h 8, 35 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 8, 35 -- Subject: Re: [Microscopy] viaWWW:FIB STEM EDX 8, 35 -- To: microscopy.listserver-at-gmail.com, "Ristau, Roger" {roger.ristau-at-uconn.edu} 8, 35 -- References: {201702181744.v1IHi58Q032489-at-microscopy.com} 8, 35 -- Cc: microscopy-at-microscopy.com 8, 35 -- From: Valery Ray {vray-at-partbeamsystech.com} 8, 35 -- Message-ID: {091f33e1-83af-a20d-62f8-4c4c404ee112-at-partbeamsystech.com} 8, 35 -- Date: Sat, 18 Feb 2017 14:17:50 -0500 8, 35 -- User-Agent: Mozilla/5.0 (Windows NT 10.0; WOW64; rv:45.0) Gecko/20100101 8, 35 -- Thunderbird/45.7.1 8, 35 -- MIME-Version: 1.0 8, 35 -- In-Reply-To: {201702181744.v1IHi58Q032489-at-microscopy.com} 8, 35 -- Content-Type: text/plain; charset=windows-1252; format=flowed 8, 35 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I have a vintage 1980 ARL-SEMQ microprobe, mostly working, that needs a home. This was the deluxe model with all of the bells and whistles available at the time (including CL imaging), and it was updated to the Advanced Microbeam automation system in the 90's. If you have any interest please contact me. I need to get it out of the lab soon to make room for new equipment. I also have nearly an entire second one in spares. If nobody takes it then it all goes to scrap.
Thanks,
Dan
-- **************************************************************************** A man who lies to himself, and believes his own lies, becomes unable to recognize truth, either in himself or in anyone else, and he ends up losing respect for himself and for others. -Fyodor Dostoevsky, novelist (1821-1881) **************************************************************************** Daniel E. Sampson Instrument Engineer A232 Earth and Marine Sciences University of California Santa Cruz, CA 95064 dsampson-at-ucsc.edu (831) 459-4992 office (831) 359-9075 cell (831) 459-3074 FAX ****************************************************
==============================Original Headers============================== 6, 44 -- From dsampson-at-ucsc.edu Tue Feb 21 10:31:38 2017 6, 44 -- Received: from mail-pg0-f53.google.com (mail-pg0-f53.google.com [74.125.83.53]) 6, 44 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v1LGVb7V014903 6, 44 -- for {microscopy-at-microscopy.com} ; Tue, 21 Feb 2017 10:31:38 -0600 6, 44 -- Received: by mail-pg0-f53.google.com with SMTP id z128so769654pgb.0 6, 44 -- for {microscopy-at-microscopy.com} ; Tue, 21 Feb 2017 08:38:26 -0800 (PST) 6, 44 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 44 -- d=ucsc.edu; s=ucsc-google; 6, 44 -- h=to:from:subject:organization:message-id:date:user-agent 6, 44 -- :mime-version:content-transfer-encoding; 6, 44 -- bh=6lK9VyYrxA1Mf5MAwloCO4ZPYHqrphlsn7RjVCfED78=; 6, 44 -- b=cyztaXke7Vg66ZaxVSk8Zwzzgq2xqKhLiKji6Hh9q+S+hi9sMLXnU4PXN0TvGXbiy7 6, 44 -- VFO1HJErDvU3u0vx1lloFNdRkxRuosfD2VDw0pDiu1b1U+LtS7vv+cFx7MEPdaT0dkJN 6, 44 -- xfG5fHZzKa7xciU3SGwXqLk7gmzIlfQuJ89AU= 6, 44 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 44 -- d=1e100.net; s=20161025; 6, 44 -- h=x-gm-message-state:to:from:subject:organization:message-id:date 6, 44 -- :user-agent:mime-version:content-transfer-encoding; 6, 44 -- bh=6lK9VyYrxA1Mf5MAwloCO4ZPYHqrphlsn7RjVCfED78=; 6, 44 -- b=M7xgdqSO1wxEBplhGGKRAtAKi6X3LfgWFszNpj65rc/xOKNMfotNARsDc3DnSvvG9/ 6, 44 -- hjp0uEAd2GMG1XkHZSnzIMSyuPZpUI4v2zx/+8hdE7a6pw39N+XfmR2dXBnYMK97D4X7 6, 44 -- wc48RzgNv40ScJrWOyItuy7X3vST3tvWeYGVJ7tgQ+NxZY3NTx/0VDIyuTUqQ79u7zrc 6, 44 -- Fkk222EO5mwApKVQ1ohpMlEx+9XLJ0mEB/3xAlm+dzTIxNx0Mr2q+cyePFEuTD3eD0Vw 6, 44 -- WQpGQlUUOlGC68dq3p7WtvrIVyahMz/rY45GF37CoTSQzccYBsW7YqtPMTHYTcMCGHYL 6, 44 -- dVag== 6, 44 -- X-Gm-Message-State: AMke39nwkaXIr+TLjhJHMjhygG9Ar+kW4nc4bvgq+hqrdZm/5YqayE27ddU2+7xLcgsWojUN 6, 44 -- X-Received: by 10.99.231.5 with SMTP id b5mr36242466pgi.80.1487695105765; 6, 44 -- Tue, 21 Feb 2017 08:38:25 -0800 (PST) 6, 44 -- Received: from dhcp-25-184.ucsc.edu (dhcp-25-184.ucsc.edu. [128.114.25.184]) 6, 44 -- by smtp.gmail.com with ESMTPSA id a24sm42096951pfh.33.2017.02.21.08.38.23 6, 44 -- for {microscopy-at-microscopy.com} 6, 44 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 6, 44 -- Tue, 21 Feb 2017 08:38:23 -0800 (PST) 6, 44 -- To: microscopy-at-microscopy.com 6, 44 -- From: Daniel Sampson {dsampson-at-ucsc.edu} 6, 44 -- Subject: Free ARL-SEMQ uProbe 6, 44 -- Organization: UC Santa Cruz 6, 44 -- Message-ID: {ab3df56e-1ccc-2e6f-db2d-9358d645b2a6-at-ucsc.edu} 6, 44 -- Date: Tue, 21 Feb 2017 08:38:24 -0800 6, 44 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.12; rv:45.0) 6, 44 -- Gecko/20100101 Thunderbird/45.7.1 6, 44 -- MIME-Version: 1.0 6, 44 -- Content-Type: text/plain; charset=utf-8; format=flowed 6, 44 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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A quick question that I hope someone could help me with.
In our daily routine analyses with our Hitachi SU6600 FE-SEM, we are getting a large component of beam drift. As in, the image seems to migrate while doing an analysis, leading to blurry images and maps. When the SEM initially turns out, we can correct for this drift with the stigmators, but eventually, the range for that tops out, and we cannot get crisp images. This occurs with well grounded samples, while doing routine imaging or EDS analyses. We typically analyze geological thin sections, but we coat with carbon and ground with either carbon or silver paint, or copper tape. I originally thought it was due to my grounding, but it used to be fine, and no matter what I do the images still drift.
However, if I put things into variable pressure mode it is fine.
It has been a while since we've done a "bakeout", so I'm just wondering if it could be something related to tip alignment, or if there are particles in the column? What can I do to check the instrument? I just worry there's something going on with the tip. How can I check that?
Thank you in advance for any info and tips! - Ivan
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Message: Hello microscopists and vendors, I was wondering what commercial software packages people are using for 3D modeling of confocal Z-stacks.I can generate beautifully detailed Z-stacks, but would like to be able to make models from the data that I could use in other applications. Specifically, I would like to generate 3D models that I could manipulate and use in movies and presentations. Any information would be appreciated, and vendors are encouraged to reply.
Thank you, -Eugene
Eugene Krueger Sr. Research Tech II Mayo Clinic
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Although it is hard to diagnose remotely, chances are that problem is not with the gun. If drift was related to electron source, then most likely it would be appearing similarly in both HV and variable pressure modes.
There is a good chance that there is some kind of dielectric contamination, particle, or a piece of fiber stuck at the bottom of objective lens. It is getting charged during high-vac operation and deflects/astigmates the beam. When you switch to variable pressure mode then ADAPT is inserted, shielding contamination from the beam, thus preventing the drift. You would need to open specimen chamber and carefully inspect bottom of the column and opening in the polepiece with mirror and a good flashlight, and try to look inside of the polepiece opening. Also take a look on outside of the insertable variable aperture for any traces of contamination or particles. Make sure that in high-vacuum mode ADAPT is fully retracted. Check resistance from polepiece to ground with ohmmeter, however strange this sounds. Also check grounding of your stage - there is some possibility that stage is electrically floating and thus getting charged in High Vac mode.
Best Wishes,
Valery Ray www.linkedin.com/in/valeryray ============================== PBS&T, MEO Engineering Company 290 Broadway, Suite 298 Methuen, MA 01844, USA Phone: +1-978-305-0479 - leave a message Mobie: +1-978-305-0479 - leave a message E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 2/21/2017 10:09 PM, microscopy.listserver-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: ibarke2-at-uwo.ca } } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } ibarke2-at-uwo.ca as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: ibarke2-at-uwo.ca Name: Ivan Barker } } Organization: Western University } } Title-Subject: [Filtered] Hitachi SU6600 FE-SEM Constant Beam Drift? } } Message: Hi all, } } A quick question that I hope someone could help me with. } } In our daily routine analyses with our Hitachi SU6600 FE-SEM, we are getting a large component of } beam drift. As in, the image seems to migrate while doing an analysis, leading to blurry images and } maps. When the SEM initially turns out, we can correct for this drift with the stigmators, but } eventually, the range for that tops out, and we cannot get crisp images. This occurs with well } grounded samples, while doing routine imaging or EDS analyses. We typically analyze geological thin } sections, but we coat with carbon and ground with either carbon or silver paint, or copper tape. I } originally thought it was due to my grounding, but it used to be fine, and no matter what I do the } images still drift. } } However, if I put things into variable pressure mode it is fine. } } It has been a while since we've done a "bakeout", so I'm just wondering if it could be something } related to tip alignment, or if there are particles in the column? What can I do to check the } instrument? I just worry there's something going on with the tip. How can I check that? } } Thank you in advance for any info and tips! } - Ivan } } Login Host: 129.100.37.41 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } }
==============================Original Headers============================== 6, 35 -- From vray-at-partbeamsystech.com Wed Feb 22 00:04:35 2017 6, 35 -- Received: from nm34-vm8.bullet.mail.bf1.yahoo.com (nm34-vm8.bullet.mail.bf1.yahoo.com [72.30.239.6]) 6, 35 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v1M64YGn003077 6, 35 -- for {microscopy-at-microscopy.com} ; Wed, 22 Feb 2017 00:04:35 -0600 6, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1487743885; bh=8YHFI87oO9r/3xrZjLxAChnbdfsxf5hkEx7i5zvfzuU=; h=Subject:To:References:From:Cc:Date:In-Reply-To:From:Subject; b=Js44PENJrGNwgqdSEb4Guy/mahduy7t7rLskpFFtw7kBjSQL+wv8mX2l60zm0ZgJXjkA8zINUAJbxstg116urJKI0nnh51/o7Pb9nExhJixZ7eSC8yiR+qI2UlH7zjCKhFEdaBVk1KCdQkA9EiHl8ePLGjOLGQ42y39YnLEh7H2u6G+ia3f0b2ifwJ3wJcitQSY7xjjzAducuHXBbNydbBJSykpuaL9NeuuARS0UY+bUx68gvGuc0hzkiIfWHKBMwWv8+bypceoYwW0H1MTr34uYcegq77XKnC8F+z0oiU9hZpDoSqQpUwzMpFaAa8CoUxamGUjBdZ2W9qMnuA84Mg== 6, 35 -- Received: from [98.139.170.178] by nm34.bullet.mail.bf1.yahoo.com with NNFMP; 22 Feb 2017 06:11:25 -0000 6, 35 -- Received: from [68.142.230.72] by tm21.bullet.mail.bf1.yahoo.com with NNFMP; 22 Feb 2017 06:11:25 -0000 6, 35 -- Received: from [127.0.0.1] by smtp229.mail.bf1.yahoo.com with NNFMP; 22 Feb 2017 06:11:25 -0000 6, 35 -- X-Yahoo-Newman-Id: 550371.29157.bm-at-smtp229.mail.bf1.yahoo.com 6, 35 -- X-Yahoo-Newman-Property: ymail-3 6, 35 -- X-YMail-OSG: vw.SJ_IVM1kcKKLBkISsO0z.5Zm6NPo80ORj6zLoy5fpANG 6, 35 -- 4ZHCLlHhEEEPvALqIQWdVVd9znpNH.MGfganbd.lkrzpxe04sxNkGMJfLBCk 6, 35 -- PU3QA3Bs9QAcIQyUhv.0IsuK3PnQnq2Ith_dvGUURtGHWulnDMDEV1QnMGap 6, 35 -- 5EmkYdmWz8XcKdtdoxlTQnrK897r1c5lcTfmQqHIORPwH4hFK.qF3EFihNKS 6, 35 -- SDwPKTxm3zvbaMkIJbuUAV.1L2HTT4dHZ8gKvf9O2_rmTpdoPMo.ssvq4.5k 6, 35 -- 4PnsQVFWTzpJhuSPHp2balPGjqWO7XRl0ZLSPfGI9_28OvT3kalA3yGmmwOg 6, 35 -- U1YzHLmHdua.PPSWdmW9aGBgF0e7ffABesIWraqvk5341sYRmUcTvlGBEgXl 6, 35 -- x1yqivW9O3MZAOSH.BROqbMkeFzN1_P6eg1jecyOtx2Jlnb7SkAosC.7Ypmh 6, 35 -- kMHbwy3NAenSBwMOvbIaugrDPk1qok2Fa3wtxog0m2f8vRTiKUCh7GrMWtTh 6, 35 -- HLF4UcTk5cHULB7bmF1pqPWxbF2Dd05CvL0HMRYrwILf_KgvpGA4ChvX5yrw 6, 35 -- vEidBUS1S 6, 35 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 6, 35 -- Subject: Re: [Microscopy] viaWWW:Hitachi SU6600 FE-SEM Constant Beam Drift? 6, 35 -- To: ibarke2-at-uwo.ca 6, 35 -- References: {201702220309.v1M396IE022755-at-microscopy.com} 6, 35 -- From: Valery Ray {vray-at-partbeamsystech.com} 6, 35 -- Cc: microscopy-at-microscopy.com 6, 35 -- Message-ID: {c23ddbb8-f915-991a-0b22-df5d95f57b3f-at-partbeamsystech.com} 6, 35 -- Date: Wed, 22 Feb 2017 01:08:08 -0500 6, 35 -- User-Agent: Mozilla/5.0 (Windows NT 10.0; WOW64; rv:45.0) Gecko/20100101 6, 35 -- Thunderbird/45.7.1 6, 35 -- MIME-Version: 1.0 6, 35 -- In-Reply-To: {201702220309.v1M396IE022755-at-microscopy.com} 6, 35 -- Content-Type: text/plain; charset=windows-1252; format=flowed 6, 35 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
We had a similar problem with a Hitachi S-2460N many years ago. I don't know how it compares to the 6600.
We had let the PM schedule lapse and got similar drifting. The engineer came in and cleaned out the liner tube, as I recall. It had built up a deposit on one side that took on a charge in hivac mode. Once it was cleaned, things were back to normal with no drift in hivac or lovac mode.
Warren Straszheim Materials Analysis and Research Lab Iowa State University
-----Original Message----- X-from: vray-at-partbeamsystech.com [mailto:vray-at-partbeamsystech.com] Sent: Wednesday, February 22, 2017 12:06 AM To: Straszheim, Warren E [BIOTC]
Mildred Dresselhaus, a professor emerita at the Massachusetts Institute of Technology whose research into the fundamental properties of carbon helped transform it into the superstar of modern materials science and the nanotechnology industry, died on Monday in Cambridge, Mass. She was 86.
A. John Mardinly, Ph.D., P.E. Retired Principal Materials Nanoanalysis Engineer
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Organization: Oakland University Eye Research Institute
Title-Subject: [Filtered] Carbon Grains on Formvar Grids or Carbon Film
Message: Sometimes it is very difficult to tell between what is sample and what is carbon grain (either grids coated with compressed carbon) or the carbon on Formvar grids. Can anyone shed light on this when one does protein analysis?
We are working at 100kV and 140Kx mag.
Thanks!
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==============================Original Headers============================== 15, 52 -- From microscopy.listserver-at-gmail.com Sat Feb 25 09:49:55 2017 15, 52 -- Received: from mail-it0-f54.google.com (mail-it0-f54.google.com [209.85.214.54]) 15, 52 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v1PFntc8008156 15, 52 -- for {microscopy-at-microscopy.com} ; Sat, 25 Feb 2017 09:49:55 -0600 15, 52 -- Received: by mail-it0-f54.google.com with SMTP id y135so46440367itc.1 15, 52 -- for {microscopy-at-microscopy.com} ; Sat, 25 Feb 2017 07:56:58 -0800 (PST) 15, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 15, 52 -- d=gmail.com; s=20161025; 15, 52 -- h=from:subject:references:reply-to:to:message-id:date:user-agent 15, 52 -- :mime-version:in-reply-to:content-transfer-encoding; 15, 52 -- bh=kBsZxknsLUuSnWdVLT9i7MxTRzmgGk2voPyx5vzdmbg=; 15, 52 -- b=lenWrIpnBlMFh+UtZ7JijNr4Nz019cAZjIaXEnKtbUvJNerx3BA47LTz+r8UXKLEom 15, 52 -- /WsATehO7V+hiKIo15FHkY6qVtoJOl+CfQdGrUoxOceoXcqaXyIT2lMtUCQjVeGqTfmM 15, 52 -- jYlOUNPzYuXN8eYHW2dsxNbIWdhPBn4KAx2fAGbWDAXRRKppsTsR0Vp5aeKr09Km+ppy 15, 52 -- ItC8TjILvwqA5TeJzFXFJ/P5CSXooojCdndi1Q9rBeoXgTuyDCv0Dzyd/kxWTg/MxdUx 15, 52 -- 9+K/OYSg9mdo02J3MX9+vNuB8IL9VFyFjTz6HlakTy3bpZ5laNbb8OIH577Dqbg9TjSn 15, 52 -- Dipg== 15, 52 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 15, 52 -- d=1e100.net; s=20161025; 15, 52 -- h=x-gm-message-state:from:subject:references:reply-to:to:message-id 15, 52 -- :date:user-agent:mime-version:in-reply-to:content-transfer-encoding; 15, 52 -- bh=kBsZxknsLUuSnWdVLT9i7MxTRzmgGk2voPyx5vzdmbg=; 15, 52 -- b=AQYiMGJvMULy6xadD3SpRiaQwpQtPPzhmVySgB2CK42qh54kFivGSa4q5eHrclN9Aq 15, 52 -- FSAQuBAQNET5ND6F3HP2XlctBtfWSEoubnjHiIwvb04fCvRKstava5kswNFzb492QP4l 15, 52 -- 1FILttj6EejUSitQ8pPFQWBo6kP0xF+aRa1h+2Fnilwhs5H76VIpsq4LtIEsgoIvMxR6 15, 52 -- e3hk/qT7BQFyhOVb+PvVM287Vu0ck0t7t3e5KyqR/k4K8E3U/jcH7G99GZBxyqbhte4G 15, 52 -- gHmMQhVqC6LC9gNUR4hcU+bbvABVW3svOT+zN9EdLVbqo9Ahb/JfjKHgs7fkK+/pguj1 15, 52 -- bm+g== 15, 52 -- X-Gm-Message-State: AMke39ko9nvhqUzTeampEip+aGCJoPSDN72TTruYPXMZYYpTPsgCJF0EivHcsf8Rf3nXUw== 15, 52 -- X-Received: by 10.36.2.65 with SMTP id 62mr7592245itu.83.1488038218093; 15, 52 -- Sat, 25 Feb 2017 07:56:58 -0800 (PST) 15, 52 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:9ce4:1fac:9eca:1af9]) 15, 52 -- by smtp.googlemail.com with ESMTPSA id y42sm4517442ioi.21.2017.02.25.07.56.57 15, 52 -- for {microscopy-at-microscopy.com} 15, 52 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 15, 52 -- Sat, 25 Feb 2017 07:56:57 -0800 (PST) 15, 52 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 15, 52 -- X-Google-Original-From: MicroscopyListserver-NoReply 15, 52 -- {microscopylistserver-noreply-at-microscopy.com} 15, 52 -- Subject: viaWWW:Carbon Grains on Formvar Grids or Carbon Film 15, 52 -- References: {201702242122.v1OLMN5g024836-at-microscopy.com} 15, 52 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 15, 52 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 15, 52 -- X-Forwarded-Message-Id: {201702242122.v1OLMN5g024836-at-microscopy.com} 15, 52 -- Message-ID: {7f734a9f-5008-db09-4794-ff2d3c55cf54-at-microscopy.com} 15, 52 -- Date: Sat, 25 Feb 2017 09:56:56 -0600 15, 52 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:45.0) 15, 52 -- Gecko/20100101 Thunderbird/45.7.1 15, 52 -- MIME-Version: 1.0 15, 52 -- In-Reply-To: {201702242122.v1OLMN5g024836-at-microscopy.com} 15, 52 -- Content-Type: text/plain; charset=windows-1252; format=flowed 15, 52 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
carbon films come in different grades one of which is 'ultra-thin' and has areas down to 3nm thick according to the publicity in catalogs. I've never done protein analysis, but you need a supporting substrate and one that is amorphous, so try and look in the holes since they may be covered with thin carbon, the thinner the better.
I have bought some silicon nitride supports that are thin and they can be plasma cleaned between uses, but they are a bit pricey.
Good luck Rob
-- Robert Keyse, DPhil Research Scientist Lehigh University
5, East Packer Avenue, Whitaker Laboratory Bethlehem, PA 18015-3194
Office (610)758-3465 Fax (610)758-4244
==============================Original Headers============================== 8, 33 -- From rok210-at-lehigh.edu Mon Feb 27 10:23:12 2017 8, 33 -- Received: from rain4.cc.lehigh.edu (rain4.cc.lehigh.edu [128.180.3.218]) 8, 33 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v1RGNCpR027453 8, 33 -- for {Microscopy-at-Microscopy.com} ; Mon, 27 Feb 2017 10:23:12 -0600 8, 33 -- Received: from [127.0.0.1] (rok210pc.mat.lehigh.edu [128.180.54.183]) 8, 33 -- (using TLSv1.2 with cipher ECDHE-RSA-AES128-GCM-SHA256 (128/128 bits)) 8, 33 -- (No client certificate requested) 8, 33 -- (Authenticated sender: rok210) 8, 33 -- by rain4.cc.lehigh.edu (Postfix) with ESMTPSA id 158B850393EF; 8, 33 -- Mon, 27 Feb 2017 11:30:21 -0500 (EST) 8, 33 -- To: Microscopy-at-Microscopy.com 8, 33 -- Cc: vakimler-at-oakland.edu 8, 33 -- From: Robert Keyse {rok210-at-lehigh.edu} 8, 33 -- Subject: Re: [Microscopy] viaWWW:Carbon Grains on Formvar Grids or Carbon Film 8, 33 -- Message-ID: {b30b53c4-17e7-5733-752e-23875ea0088d-at-lehigh.edu} 8, 33 -- Date: Mon, 27 Feb 2017 11:30:21 -0500 8, 33 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:45.0) Gecko/20100101 8, 33 -- Thunderbird/45.7.1 8, 33 -- MIME-Version: 1.0 8, 33 -- Content-Type: text/plain; charset=utf-8; format=flowed 8, 33 -- Content-Transfer-Encoding: 7bit 8, 33 -- X-Antivirus: avast! (VPS 170227-0, 02/27/2017), Outbound message 8, 33 -- X-Antivirus-Status: Clean 8, 33 -- X-Virus-Scanned: clamav-milter 0.99.2 at rain4.CC.lehigh.EDU 8, 33 -- X-Virus-Status: Clean 8, 33 -- X-Spam-Status: No, score=-4.0 required=5.0 tests=ALL_TRUSTED,LU_FORGED_SENDER, 8, 33 -- RP_MATCHES_RCVD autolearn=disabled version=3.4.1 8, 33 -- X-Spam-Report: 8, 33 -- * -5.0 ALL_TRUSTED Passed through trusted hosts only via SMTP 8, 33 -- * -0.0 RP_MATCHES_RCVD Envelope sender domain matches handover relay domain 8, 33 -- * 1.0 LU_FORGED_SENDER No description available. 8, 33 -- X-Spam-Checker-Version: SpamAssassin 3.4.1 (2015-04-28) on 8, 33 -- freddie.cc.lehigh.edu ==============================End of - Headers==============================
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Email: allan.mitchell-at-otago.ac.nz Name: Allan Mitchell
Organization: Otago centre for Electron Microscopy, University of Otago
Title-Subject: [Filtered] Ultramicrotomes and gridstainers
Message: We are in the process of trying to obtain funding to replace one of our ultramicrotomes (Ultracut E) and our aged LKB Ultrostainer. We are looking at - RMC Boeckeler ultramicrotme PT-PCZ and the RMC Boeckeler QG-3100 TEM Stainer, or
- Leica Ultracut EM UC7 ultramicrotome and Leica EM AC20 grid-stainer.
I would be keen to hear of peoples experiences with any of these instruments, positives /negatives.
Many thanks
Allan
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Email: duraine-at-bcm.edu Name: Lita Duraine
Organization: Howard Hughes Medical Institute
Title-Subject: [Filtered] Re: viaWWW:Ultramicrotomes and gridstainers
Message: Hi Allan,
I totally support the Leica Ultracut EM UC7 microtome. We bought one in 2013 and it has worked efficiently ever since. It is very user friendly and the program settings are easy to manage. Hardly any break-in time. The thickness settings stay consistent. You also have a wide latitude for cutting speeds. There are several light settings especially helpful when I am hand trimming a block and then want to go immediately to cutting thins. This instrument is so accurate that it can cut silver sections effortlessly even on the 11th floor of our building! We loved it so much that we bought a second one two years later when we expanded our lab. Customer service is another reason why I use Leica. We had a small static shock occurring in the new room where we put the microtome. Not sure what was causing it, I called Leica. Immediately, they sent an engineer to our location in Houston and checked everything on the UC7. Several issues were found within the room actually, and everything turned out great. The UC7 is a workhorse, and never skips a beat. I highly recommend the Leica UC7 Ultramicrotome.
Lita Duraine Certified Electron Microscopist Howard Hughes Medical Institute Houston, TX 77030
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Title-Subject: [Filtered] The Materials Ultramicrotomy Workshop
Message: Three days of hands-on training for technicians, researchers, and students who want to apply a faster and cleaner preparation method that provides samples with uniform thickness, no embedded contamination, and is cheaper than a FIB.
Details: Wednesday - Friday April 12-14, 2017 8:00 a.m. - 4:30 p.m. EMS Microscopy Academy Hatfield, Pennsylvania, USA
Instructors: Helmut Gnaegi, Diatome Ltd., Switzerland Robert Ranner, Leica, Austria Class size is limited! Register now! For more information, visit: http://www.emsdiasum.com/microscopy/academy/courses/materials_ultramicrotomy.aspx
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Our Hitachi S-2700 SEM will no longer produce an image. We can only see a series of vertical lines of varying intensity with both secondary electron and backscatter detectors. We do have an electron beam - there are changes in intensity of the vertical lines during adjustment of filament current, beam tilt, beam horizon, changes in the lines if we move the specimen. We do lose the image if the HV is turned off on the secondary electron detector. A very similar pattern is seen with the backscatter detector, so it does not appear to be a detector issue.
We have exchanged circuit boards and even the entire column (!) from a second identical scope used for parts. A commercial technician was unable to correct the problem. His best guess is that one or more of the lenses are not functioning.
Any suggestions as to what the problem is? I can send images off-line.
Jeffrey Thompson, Ph.D. Professor of Biology California State University 5500 University Pkwy San Bernardino, CA 92407 909-537-5315
==============================Original Headers============================== 7, 49 -- From jthompso-at-csusb.edu Thu Mar 2 19:06:25 2017 7, 49 -- Received: from NAM03-BY2-obe.outbound.protection.outlook.com (mail-by2nam03on0075.outbound.protection.outlook.com [104.47.42.75]) 7, 49 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v2316P03021393 7, 49 -- for {Microscopy-at-microscopy.com} ; Thu, 2 Mar 2017 19:06:25 -0600 7, 49 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=csusb.edu; 7, 49 -- s=selector1; h=From:Date:Subject:Message-ID:Content-Type:MIME-Version; 7, 49 -- bh=2DXHl+sdv7lZfoI1ZlAV7dldcoThOfcItUM8cr4e4Yg=; 7, 49 -- b=ItH5GzlWhXr5lMs9/qNc4uSnMCV7/fCa5nYZJSY1jKHojRCLtbNETh/vXuYiUmC/yoCWF1KbpA4Cm5gR5FhCj85Sn0OlkEwjp5nNW2gBMjGiqbU+42e0tzNtfK9lgXGFV+a/wyCFSdayxhfFkLeSEwfcwLizCNu58xUFMvn4Ikw= 7, 49 -- Received: from CY4PR08MB2869.namprd08.prod.outlook.com (10.173.59.147) by 7, 49 -- CY4PR08MB2871.namprd08.prod.outlook.com (10.173.59.149) with Microsoft SMTP 7, 49 -- Server (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_256_CBC_SHA384_P384) id 7, 49 -- 15.1.933.12; Fri, 3 Mar 2017 01:13:47 +0000 7, 49 -- Received: from CY4PR08MB2869.namprd08.prod.outlook.com ([10.173.59.147]) by 7, 49 -- CY4PR08MB2869.namprd08.prod.outlook.com ([10.173.59.147]) with mapi id 7, 49 -- 15.01.0933.020; Fri, 3 Mar 2017 01:13:47 +0000 7, 49 -- From: Jeffrey Thompson {jthompso-at-csusb.edu} 7, 49 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 7, 49 -- Subject: SEM Need help in understanding loss of image generation 7, 49 -- Thread-Topic: SEM Need help in understanding loss of image generation 7, 49 -- Thread-Index: AdKTusYGL9em7MiDQpOEkIYERiIIyQ== 7, 49 -- Date: Fri, 3 Mar 2017 01:13:47 +0000 7, 49 -- Message-ID: {CY4PR08MB286955557359A0BF5875540FCC2B0-at-CY4PR08MB2869.namprd08.prod.outlook.com} 7, 49 -- Accept-Language: en-US 7, 49 -- Content-Language: en-US 7, 49 -- X-MS-Has-Attach: 7, 49 -- X-MS-TNEF-Correlator: 7, 49 -- authentication-results: microscopy.com; dkim=none (message not signed) 7, 49 -- header.d=none;microscopy.com; dmarc=none action=none header.from=csusb.edu; 7, 49 -- x-originating-ip: [2607:f380:a61:142:5583:ac34:b43d:3ef6] 7, 49 -- x-ms-office365-filtering-correlation-id: 26778956-7cca-48ee-dd69-08d461d28b35 7, 49 -- x-microsoft-antispam: UriScan:;BCL:0;PCL:0;RULEID:(22001);SRVR:CY4PR08MB2871; 7, 49 -- x-microsoft-exchange-diagnostics: 1;CY4PR08MB2871;7:rHS23JLozCsvFvo6phag6YemA56+clfV4rUPzDzQFY3NAqQSpVYtmBAOD5jSvxl8S/e2ourHvZApghyjoYRRY8Yq6cBuVKOQ6o8jHQ0tvKSqR9zGPKxCpmcmEGYSYLowGN9xDM0fS8yoHnpVwoH1ByQS0e1K442YN53nI3y/jv2Ur6xkOObcQmAUv+hhLNw0KRFWCxWLKM/zP0uQSui2YG8SOyekfRXzW33lZc1slb0y6obbxDehbW7027o/+FI+Tvrxbir+/dYbH6WHOi/8V+2SQsGEbPhRnlyktrO/TRTq4SNIAw3eWz62GreW3q3DMITuu7GTDqB9TAatF2jBLA==;20:FBEKbZT5i0wXScOUNyIugqVbQUjmHgFIfXKT2lgYME4k+z99AAECHORHR9o907/ivCzudIgqpsG6MDtbIXEhVqR5A7vJJZKpJogcHTCT4DokeVZLB1VDVrvyTTjAOj/BWXIpZwT4HKufvDYPtJMlQkpoyNXEHMcSu5q76tKAgjs= 7, 49 -- x-microsoft-antispam-prvs: {CY4PR08MB2871632F020A7C5B5B32C212CC2B0-at-CY4PR08MB2871.namprd08.prod.outlook.com} 7, 49 -- x-exchange-antispam-report-test: UriScan:; 7, 49 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:(6040375)(601004)(2401047)(8121501046)(5005006)(3002001)(10201501046)(6041248)(20161123558025)(20161123562025)(20161123560025)(20161123564025)(20161123555025)(6072148);SRVR:CY4PR08MB2871;BCL:0;PCL:0;RULEID:;SRVR:CY4PR08MB2871; 7, 49 -- x-forefront-prvs: 0235CBE7D0 7, 49 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10009020)(6009001)(7916002)(39410400002)(39830400002)(39450400003)(81166006)(74316002)(305945005)(122556002)(7736002)(189998001)(92566002)(3280700002)(2906002)(75432002)(88552002)(53936002)(3660700001)(2900100001)(2501003)(450100001)(86362001)(77096006)(8936002)(110136004)(38730400002)(8676002)(6506006)(50986999)(54356999)(7696004)(55016002)(102836003)(6116002)(6436002)(99286003)(5640700003)(2351001)(25786008)(6916009)(33656002)(5660300001)(9686003);DIR:OUT;SFP:1101;SCL:1;SRVR:CY4PR08MB2871;H:CY4PR08MB2869.namprd08.prod.outlook.com;FPR:;SPF:None;MLV:sfv;LANG:en; 7, 49 -- spamdiagnosticoutput: 1:99 7, 49 -- spamdiagnosticmetadata: NSPM 7, 49 -- Content-Type: text/plain; charset="us-ascii" 7, 49 -- MIME-Version: 1.0 7, 49 -- X-OriginatorOrg: csusb.edu 7, 49 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 03 Mar 2017 01:13:47.0310 7, 49 -- (UTC) 7, 49 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted 7, 49 -- X-MS-Exchange-CrossTenant-id: d73b9eaa-07c9-47c4-a6ce-f13bee0e8117 7, 49 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: CY4PR08MB2871 7, 49 -- Content-Transfer-Encoding: 8bit 7, 49 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v2316P03021393 ==============================End of - Headers==============================
Please feel free to send or post images to look at, but most likely you have a simple deflection problem. Remember that in SEM lenses are only used to form the beam, but magnification (and formation of image) comes from ratio between area physicall scanned by electron beam on surface of the sample and area of the CRT screen (or digital image) representing the image. If you see only vertical lines then chances are that primary electron beam is not scanned in Y direction over the sample, so in every line of image you are seeing information from the same exact line physically scanned by electron beam on the sample. Find a local tech who knows how to use oscilloscope and able to figure out amplifiers driving inductive loads (scan coils)....
Valery Ray www.linkedin.com/in/valeryray ============================== PBS&T, MEO Engineering Company 290 Broadway, Suite 298 Methuen, MA 01844, USA Phone: +1-978-296-5063 - leave a message Mobie: +1-978-305-0479 - leave a message E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 3/2/2017 8:07 PM, jthompso-at-csusb.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Our Hitachi S-2700 SEM will no longer produce an image. We can only see a series of vertical lines of varying intensity with both secondary electron and backscatter detectors. We do have an electron beam - there are changes in intensity of the vertical lines during adjustment of filament current, beam tilt, beam horizon, changes in the lines if we move the specimen. We do lose the image if the HV is turned off on the secondary electron detector. A very similar pattern is seen with the backscatter detector, so it does not appear to be a detector issue. } } We have exchanged circuit boards and even the entire column (!) from a second identical scope used for parts. A commercial technician was unable to correct the problem. His best guess is that one or more of the lenses are not functioning. } } Any suggestions as to what the problem is? I can send images off-line. } } } Jeffrey Thompson, Ph.D. } Professor of Biology } California State University } 5500 University Pkwy } San Bernardino, CA 92407 } 909-537-5315 } } } } ==============================Original Headers============================== } 7, 49 -- From jthompso-at-csusb.edu Thu Mar 2 19:06:25 2017 } 7, 49 -- Received: from NAM03-BY2-obe.outbound.protection.outlook.com (mail-by2nam03on0075.outbound.protection.outlook.com [104.47.42.75]) } 7, 49 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v2316P03021393 } 7, 49 -- for {Microscopy-at-microscopy.com} ; Thu, 2 Mar 2017 19:06:25 -0600 } 7, 49 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=csusb.edu; } 7, 49 -- s=selector1; h=From:Date:Subject:Message-ID:Content-Type:MIME-Version; } 7, 49 -- bh=2DXHl+sdv7lZfoI1ZlAV7dldcoThOfcItUM8cr4e4Yg=; } 7, 49 -- b=ItH5GzlWhXr5lMs9/qNc4uSnMCV7/fCa5nYZJSY1jKHojRCLtbNETh/vXuYiUmC/yoCWF1KbpA4Cm5gR5FhCj85Sn0OlkEwjp5nNW2gBMjGiqbU+42e0tzNtfK9lgXGFV+a/wyCFSdayxhfFkLeSEwfcwLizCNu58xUFMvn4Ikw= } 7, 49 -- Received: from CY4PR08MB2869.namprd08.prod.outlook.com (10.173.59.147) by } 7, 49 -- CY4PR08MB2871.namprd08.prod.outlook.com (10.173.59.149) with Microsoft SMTP } 7, 49 -- Server (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_256_CBC_SHA384_P384) id } 7, 49 -- 15.1.933.12; Fri, 3 Mar 2017 01:13:47 +0000 } 7, 49 -- Received: from CY4PR08MB2869.namprd08.prod.outlook.com ([10.173.59.147]) by } 7, 49 -- CY4PR08MB2869.namprd08.prod.outlook.com ([10.173.59.147]) with mapi id } 7, 49 -- 15.01.0933.020; Fri, 3 Mar 2017 01:13:47 +0000 } 7, 49 -- From: Jeffrey Thompson {jthompso-at-csusb.edu} } 7, 49 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} } 7, 49 -- Subject: SEM Need help in understanding loss of image generation } 7, 49 -- Thread-Topic: SEM Need help in understanding loss of image generation } 7, 49 -- Thread-Index: AdKTusYGL9em7MiDQpOEkIYERiIIyQ== } 7, 49 -- Date: Fri, 3 Mar 2017 01:13:47 +0000 } 7, 49 -- Message-ID: {CY4PR08MB286955557359A0BF5875540FCC2B0-at-CY4PR08MB2869.namprd08.prod.outlook.com} } 7, 49 -- Accept-Language: en-US } 7, 49 -- Content-Language: en-US } 7, 49 -- X-MS-Has-Attach: } 7, 49 -- X-MS-TNEF-Correlator: } 7, 49 -- authentication-results: microscopy.com; dkim=none (message not signed) } 7, 49 -- header.d=none;microscopy.com; 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Hi Jeffrey, I'm agree with Valery, a deflection circuit is faulty. A deflection problem is also possible from the screen circuit. This can be check by connecting the video output on an external monitor. This microscope has probably two stages of deflectors to scan the beam and each of them set with X and Y deflection coils. The most common problem is coming from power transistor on horizontal circuit. This component is more sollicited because the scan speed horizontal is faster than the vertical. This electronic is sensitive to temperature of the room and of the cooling water. The fastest is the speed the more those transistors are working, if you can set your microscope to slow scan mode when you don't use, it's safety for deflection circuit. High magnification position is also good.
Nicolas STEPHANT
Universit de Nantes Institut Jean Rouxel Service de microscopie lectronique balayage et microanalyse 2 rue de la Houssinire BP 92208 44322 Nantes cdex 3
Tl : 02 40 37 64 26 Ml : nicolas.stephant-at-univ-nantes.fr Web : http://cnrs-imn.fr
"Le monde n'existe que pour autant que nous sommes capables d'en produire une image" C.G Jung
Le 03/03/2017 02:21, jthompso-at-csusb.edu a crit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Our Hitachi S-2700 SEM will no longer produce an image. We can only see a series of vertical lines of varying intensity with both secondary electron and backscatter detectors. We do have an electron beam - there are changes in intensity of the vertical lines during adjustment of filament current, beam tilt, beam horizon, changes in the lines if we move the specimen. We do lose the image if the HV is turned off on the secondary electron detector. A very similar pattern is seen with the backscatter detector, so it does not appear to be a detector issue. } } We have exchanged circuit boards and even the entire column (!) from a second identical scope used for parts. A commercial technician was unable to correct the problem. His best guess is that one or more of the lenses are not functioning. } } Any suggestions as to what the problem is? I can send images off-line. } } } Jeffrey Thompson, Ph.D. } Professor of Biology } California State University } 5500 University Pkwy } San Bernardino, CA 92407 } 909-537-5315 } } } } ==============================Original Headers============================== } 7, 49 -- From jthompso-at-csusb.edu Thu Mar 2 19:06:25 2017 } 7, 49 -- Received: from NAM03-BY2-obe.outbound.protection.outlook.com (mail-by2nam03on0075.outbound.protection.outlook.com [104.47.42.75]) } 7, 49 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v2316P03021393 } 7, 49 -- for {Microscopy-at-microscopy.com} ; Thu, 2 Mar 2017 19:06:25 -0600 } 7, 49 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=csusb.edu; } 7, 49 -- s=selector1; h=From:Date:Subject:Message-ID:Content-Type:MIME-Version; } 7, 49 -- bh=2DXHl+sdv7lZfoI1ZlAV7dldcoThOfcItUM8cr4e4Yg=; } 7, 49 -- b=ItH5GzlWhXr5lMs9/qNc4uSnMCV7/fCa5nYZJSY1jKHojRCLtbNETh/vXuYiUmC/yoCWF1KbpA4Cm5gR5FhCj85Sn0OlkEwjp5nNW2gBMjGiqbU+42e0tzNtfK9lgXGFV+a/wyCFSdayxhfFkLeSEwfcwLizCNu58xUFMvn4Ikw= } 7, 49 -- Received: from CY4PR08MB2869.namprd08.prod.outlook.com (10.173.59.147) by } 7, 49 -- CY4PR08MB2871.namprd08.prod.outlook.com (10.173.59.149) with Microsoft SMTP } 7, 49 -- Server (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_256_CBC_SHA384_P384) id } 7, 49 -- 15.1.933.12; 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==============================Original Headers============================== 7, 26 -- From Nicolas.Stephant-at-univ-nantes.fr Fri Mar 3 02:02:10 2017 7, 26 -- Received: from mail2.cnrs-imn.fr (mail2.cnrs-imn.fr [193.52.97.4]) 7, 26 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v23829Bi015455 7, 26 -- for {microscopy-at-microscopy.com} ; Fri, 3 Mar 2017 02:02:10 -0600 7, 26 -- Received: from pro-smtp.cnrs-imn.fr (smtp.cnrs-imn.fr [194.214.57.230]) 7, 26 -- by mail2.cnrs-imn.fr (8.14.4/8.14.4/DG) with ESMTP id v2389TKR005436; 7, 26 -- Fri, 3 Mar 2017 09:09:29 +0100 7, 26 -- Received: from [10.2.6.11] (pc11.cnrs-imn.fr [10.2.6.11]) 7, 26 -- by pro-smtp.cnrs-imn.fr (8.14.4/8.14.4/Debian-8+deb8u1) with ESMTP id v2389SgX071606 7, 26 -- (version=TLSv1/SSLv3 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128 verify=NOT); 7, 26 -- Fri, 3 Mar 2017 09:09:29 +0100 7, 26 -- Reply-To: Nicolas.Stephant-at-univ-nantes.fr 7, 26 -- Subject: Re: SEM Need help in understanding loss of image generation 7, 26 -- References: {201703030121.v231Llu4014233-at-microscopy.com} 7, 26 -- To: jthompso-at-csusb.edu, microscopy-at-microscopy.com 7, 26 -- From: Nicolas Stephant {Nicolas.Stephant-at-univ-nantes.fr} 7, 26 -- Message-ID: {4f7a1e0d-7a2f-60ea-6120-ba90d0e9fbb3-at-univ-nantes.fr} 7, 26 -- Date: Fri, 3 Mar 2017 09:09:59 +0100 7, 26 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:45.0) Gecko/20100101 7, 26 -- Thunderbird/45.7.0 7, 26 -- MIME-Version: 1.0 7, 26 -- In-Reply-To: {201703030121.v231Llu4014233-at-microscopy.com} 7, 26 -- Content-Type: text/plain; charset=windows-1252; format=flowed 7, 26 -- Content-Transfer-Encoding: 8bit 7, 26 -- X-Miltered: at b-mail2 with ID 58B924B9.000 by Joe's j-chkmail (http : // j-chkmail dot ensmp dot fr)! 7, 26 -- X-j-chkmail-Enveloppe: 58B924B9.000 from smtp.cnrs-imn.fr/smtp.cnrs-imn.fr/194.214.57.230/pro-smtp.cnrs-imn.fr/ {Nicolas.Stephant-at-univ-nantes.fr} ==============================End of - Headers==============================
Dear Listers, a friend of mine is interested in buying a SEM with a LaB6 emitter, for imaging and, above all, analytical applications: EDS and WDS. However, he was told that LaB6 emitters are not a good choice for analytical applications in a SEM, mainly due to stability issues, which - if I got it correctly - would require a long wait time before reliable spectra could be acquired. I have no experience with such a machine, so I can't advice him about this. Maybe some of you can help me. Thank you in advance. Regards
==============================Original Headers============================== 6, 46 -- From dcristofori-at-unive.it Fri Mar 3 10:22:45 2017 6, 46 -- Received: from mail-wr0-f173.google.com (mail-wr0-f173.google.com [209.85.128.173]) 6, 46 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v23GMiPM018377 6, 46 -- for {microscopy-at-microscopy.com} ; Fri, 3 Mar 2017 10:22:45 -0600 6, 46 -- Received: by mail-wr0-f173.google.com with SMTP id u48so77244866wrc.0 6, 46 -- for {microscopy-at-microscopy.com} ; Fri, 03 Mar 2017 08:30:10 -0800 (PST) 6, 46 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 46 -- d=unive-it.20150623.gappssmtp.com; s=20150623; 6, 46 -- h=from:to:subject:message-id:date:user-agent:mime-version 6, 46 -- :content-transfer-encoding; 6, 46 -- bh=QQOwdriEmZR1wQYL8Gco610AhFpwwY9mqzxT0DxSf8M=; 6, 46 -- b=M49XawHtmLofs3N57L/N3GtPKhNdWKKnwVPh6oYDlLbkg34ryQPc3BC3herZ1yGK9I 6, 46 -- e+wrlpyVJFNcrjXwPaOtmvZJHH7F/6/lNmEhtdd3AFOENh9wljyDptP5S+bjQJeSvbm/ 6, 46 -- 6QTw0Nc/selXiqeH1po4zQ3QIOSFR5vaueckyZJzzdCqNSITVLPO2s3A+5TRoFke+QG4 6, 46 -- ZUVykpzfMbKAfqGO+ASJGSv07iDoEU5LFUOHPp175+i1Eh8/PxIuHCNPKZPxGisBHiYY 6, 46 -- M39UKVSDwubwbe2hev5c7FGhW468hmLAh2zfkNaTyDcTDHPw4qZ/xFJbSZZXvgQ17v4I 6, 46 -- Gd9A== 6, 46 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 46 -- d=1e100.net; s=20161025; 6, 46 -- h=x-gm-message-state:from:to:subject:message-id:date:user-agent 6, 46 -- :mime-version:content-transfer-encoding; 6, 46 -- bh=QQOwdriEmZR1wQYL8Gco610AhFpwwY9mqzxT0DxSf8M=; 6, 46 -- b=jvGpQU1r86GyTGoj9JEP6GuJVM6Fr6hzl00gZWgI6knwDUsi49Zh+gQDGUPLcQm6v0 6, 46 -- s3dEiXIkEDtSbDoGvQxU+SZtDZX06iRr6NTr/rP6s9tinSGYOgHUiLKLHJm831xOKiXJ 6, 46 -- B/Q9QwpcU0sZEXWuNx77EZ5h8l3w5QmzlQNhwch3WwAozPCsr+aFF2uW8EK3EBs5Dxhu 6, 46 -- hl/jR7cbomQTPmXJVqomFysTgRwjzG9p04HACpYiWgSLLe0DKAxks9CdUDMhkEj360sx 6, 46 -- Kg2MNVroj4vz9moMa4u7H3qyZCCfJFLtXAih474cQIr+LPeXV96LLLheNNiKjiNauNgn 6, 46 -- /k6A== 6, 46 -- X-Gm-Message-State: AMke39kFDIoB48acGUfpQ1fhWSsUYWTnwUsJSh8w+5Y9SvmFAH/VhdNTvaev9nEd7hKvbagf 6, 46 -- X-Received: by 10.223.153.17 with SMTP id x17mr3372369wrb.55.1488558608506; 6, 46 -- Fri, 03 Mar 2017 08:30:08 -0800 (PST) 6, 46 -- Received: from [157.138.23.17] (battagliarin.dcf.unive.it. [157.138.23.17]) 6, 46 -- by smtp.gmail.com with ESMTPSA id 11sm15805757wrb.10.2017.03.03.08.30.06 6, 46 -- for {microscopy-at-microscopy.com} 6, 46 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 6, 46 -- Fri, 03 Mar 2017 08:30:07 -0800 (PST) 6, 46 -- From: Davide Cristofori {dcristofori-at-unive.it} 6, 46 -- To: microscopy-at-microscopy.com 6, 46 -- Subject: SEM: are LaB6 emitters fit for EDS and WDS? 6, 46 -- Message-ID: {b75d8a9c-5ac7-9147-523f-d53f3c49537f-at-unive.it} 6, 46 -- Date: Fri, 3 Mar 2017 17:30:31 +0100 6, 46 -- User-Agent: Mozilla/5.0 (Windows NT 10.0; WOW64; rv:45.0) Gecko/20100101 6, 46 -- Thunderbird/45.7.1 6, 46 -- MIME-Version: 1.0 6, 46 -- Content-Type: text/plain; charset=iso-8859-15; format=flowed 6, 46 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
From medinaluca1-at-gmail.com Fri Mar 3 16:42:07 2017 Return-Path: {medinaluca1-at-gmail.com} Received: from gmail.com (61-222-131-125.HINET-IP.hinet.net [61.222.131.125]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v23Mg4Dt021132 for {microscopylistserverarchive6-at-microscopy.com} ; Fri, 3 Mar 2017 16:42:06 -0600 Message-ID: {8F6BD8DD.8098AF46-at-gmail.com}
Hi
I know of no problems with LaB6 emitters with regard to stability, provide that the vacuum level in the electron gun is suitable for their use. Could you be confusing the instability problem with cold field emitter instruments, where their natural emission is prone to early and late instabilities during long operating session. Regards
Steve
Steve Chapman FRMS Protrain for Consultancy and Training in Electron Microscopy +44 (0)7711 606967 web www.emcourses.com
-----Original Message----- X-from: dcristofori-at-unive.it [mailto:dcristofori-at-unive.it] Sent: 03 March 2017 16:24 To: protrain-at-emcourses.com
Dear Listers, a friend of mine is interested in buying a SEM with a LaB6 emitter, for imaging and, above all, analytical applications: EDS and WDS. However, he was told that LaB6 emitters are not a good choice for analytical applications in a SEM, mainly due to stability issues, which - if I got it correctly - would require a long wait time before reliable spectra could be acquired. I have no experience with such a machine, so I can't advice him about this. Maybe some of you can help me. Thank you in advance. Regards
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Dear List I am looking for SEM images of the various types/states of human white blood cells. Does anybody know any accessible source of such images that is scientifically sound? Thanks for your time yorgos
Tel/fax +30 210 8957677 mobile +30 6945 107477 www.eikonika.netwww.aim.cat *************************************
==============================Original Headers============================== 5, 20 -- From eikonika-at-otenet.gr Sun Mar 5 01:52:51 2017 5, 20 -- Received: from echidna.otenet.gr (smtp-out33.otenet.gr [83.235.69.33]) 5, 20 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v257qp7s009600 5, 20 -- for {microscopy-at-microscopy.com} ; Sun, 5 Mar 2017 01:52:51 -0600 5, 20 -- Received: from ozymandias (ppp-2-84-169-220.home.otenet.gr [2.84.169.220]) 5, 20 -- by echidna.otenet.gr (ESMTP) with ESMTPSA 5, 20 -- for {microscopy-at-microscopy.com} ; Sun, 5 Mar 2017 10:00:19 +0200 (EET) 5, 20 -- From: "Yorgos Nikas" {eikonika-at-otenet.gr} 5, 20 -- To: {microscopy-at-microscopy.com} 5, 20 -- Subject: leucocyte types in SEM 5, 20 -- Date: Sun, 5 Mar 2017 10:00:21 +0200 5, 20 -- Message-ID: {000001d29586$89f24d00$9dd6e700$-at-otenet.gr} 5, 20 -- MIME-Version: 1.0 5, 20 -- Content-Type: text/plain; 5, 20 -- charset="iso-8859-1" 5, 20 -- X-Mailer: Microsoft Outlook 14.0 5, 20 -- Thread-Index: AdKVhaNEwTFdRgEUSHmWLgMsN4X4yQ== 5, 20 -- Content-Language: en-us 5, 20 -- Content-Transfer-Encoding: 8bit 5, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v257qp7s009600 ==============================End of - Headers==============================
From donahenr845-at-gmail.com Mon Mar 6 03:48:07 2017 Return-Path: {donahenr845-at-gmail.com} Received: from gmail.com ([220.125.146.235]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v269m4la008671 for {microscopylistserverarchive6-at-microscopy.com} ; Mon, 6 Mar 2017 03:48:06 -0600 Message-ID: {912986DA.0519D8D0-at-gmail.com}
X-from: kuryu-at-rockefeller.edu
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Email: kuryu-at-rockefeller.edu Name: Hiro Uryu
Organization: rockefeller university
Title-Subject: [Filtered] Do you know about these electon dese bodies in ER?
Message: Dear list,
I was searching for a significance of electron dense bodies that appeared to be spherical and located inside of ER. These may be heavily electron dense with or without an electron opaque core. So far I found one image in the following link, indicting a type of structured that I have seen. http://classes.kumc.edu/som/CellBiology/organelles/smoother/index.html Does anyone have an idea what they might be and what the biological significance might be associated with? I would love to hear your understanding of these dense bodies. I would be happy to share my image if you would connect with me off site. Many thanks,
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We use a Leica EM UC6 for 12 years now, it is solid as a rock and precise like a swiss clock. I just had to put one drop of oil in the gears mechanism after 10 years (3 screws to unscrew, I was able to do it myself!), which I accomplished very bravely :-D Now I am troubled because I don't know anymore if the most loyal companion of men is the dog or a Leica ultramicrotome!
Regards, Stephane
-------------------------------------------- On Wed, 3/1/17, microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} wrote:
Subject: [Microscopy] viaWWW:Ultramicrotomes and gridstainers To: nizets2-at-yahoo.com Date: Wednesday, March 1, 2017, 1:16 PM
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X-from: allan.mitchell-at-otago.ac.nz
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Email: allan.mitchell-at-otago.ac.nz Name: Allan Mitchell
Organization: Otago centre for Electron Microscopy, University of Otago
Title-Subject: [Filtered] Ultramicrotomes and gridstainers
Message: We are in the process of trying to obtain funding to replace one of our ultramicrotomes (Ultracut E) and our aged LKB Ultrostainer. We are looking at - RMC Boeckeler ultramicrotme PT-PCZ and the RMC Boeckeler QG-3100 TEM Stainer, or
- Leica Ultracut EM UC7 ultramicrotome and Leica EM AC20 grid-stainer.
I would be keen to hear of peoples experiences with any of these instruments, positives /negatives.
Many thanks
Allan
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We are in process of decommissioning our JEOL 6100 ( 25 yo), and it will be removed from lab in two weeks to make room for another equipment. The tool was last used over a year ago in good working condition, and had been under PM contract since new. It has an Oxford EDS (ISIS) unit, IR chamber camera, and specimen current meter on it. Please contact me if you are interested in taking it as a whole, or parts of it. You will need to pay for the packing and shipping of the tool from Boston to your location. Thanks.
Anlee Krupp Laboratory Manager Precision Measurement Laboratory Boston University Photonics Center 8 Saint Mary's Street, Boston, MA 02215 Phone: (617) 353-9044 Email: ahk-at-bu.edu
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Email: lon.nelson-at-leica-microsystems.com Name: LON NELSON
Leica Microsystems and BitesizeBio have organized a free web-seminar in March that may be of interest to you:
Revealing Cellular Dynamics with Millisecond Precision The New Tool That Turned Electron Micrographs in Motion Picture of Neural Communication
Presenters: Dr. Shigeki Watanabe Johns Hopkins School of Medicine
Dr. Frédéric Leroux Leica Microsystems
What if you can dissect the cellular dynamics with millisecond precision? What if you can unravel the morphological transformation of a neuron millisecond by millisecond using electron microscopy? Could this be even possible?
In this webinar, we will talk about how optogenetics coupled with high-pressure freezing can make this possible. We will discuss how to implement electrical stimulation and why it is superior to light stimulation. We will also discuss the importance of sample processing and the challenges you would face while freezing different types of samples.
Very best regards,
Lon Nelson Director of Sales Microscopy lon.nelson-at-leica-microsystems.com M +1 224-628-2467 | F +1 847-607-3160 Leica Microsystems, Inc. 1700 Leider Ln | Buffalo Grove, IL 60089 (USA)
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Dear Listers, thanks to all of you who answered my question. LaB6 emitters are unanimously considered stable, yet some of you pointed out that the filament has to be kept heated in order to assure a stable emission, even when not working, e.g. overnight.
I'd like to figure out how common is this approach, and the life of LaB6 emitters operated in this way. It would be very interesting and useful to know your experience also about this point, if you want to share it. Thanks in advance Regards
Compare FEI 12T and FEI Quanta 400 ESEM, both tungsten emitters (exchangeable). Quanta 400 ESEM Average life = 60-80 hr When Quanta vented to change specimen, entire column is vented with AIR (could be better with N2, but not often) Tecnai 12T (120kV) Average life = 1000 hr** When change specimen (single tilt) current condition of filament is maintained until vacuum in specimen chamber has recovered.
Quanta filament is OFF overnight. Tecnai filament is under low current and minimum vacuum pressure 24/7 (10-8 with no break in vacuum in life).
Hope this helps too.
Fred Monson
Frederick C. Monson, PhD Technical Director Center for Microanalysis and Imaging, Research and Training (CMIRT) West Chester University of PA Geology-Astronomy 750 South Church St. West Chester, PA, 19383 fmonson-at-wcupa.edu 610-738-0437(Work)
-----Original Message----- X-from: dcristofori-at-unive.it [mailto:dcristofori-at-unive.it] Sent: Friday, March 10, 2017 10:18 AM To: Monson, Frederick {FMonson-at-wcupa.edu}
Dear Listers, thanks to all of you who answered my question. LaB6 emitters are unanimously considered stable, yet some of you pointed out that the filament has to be kept heated in order to assure a stable emission, even when not working, e.g. overnight.
I'd like to figure out how common is this approach, and the life of LaB6 emitters operated in this way. It would be very interesting and useful to know your experience also about this point, if you want to share it. Thanks in advance Regards
LaB6 emitters, like any other source, outgas when first heated and may then be unstable. A way of getting round this is to run the filament at a very low current, just to keep it warm, even if the instrument is not being used. I do not know if they do it now, but JEOL with LaB6 systems set the filament heating at half value when you "turned it off". At very low emission there is no source evaporation, so the filament life does not suffer.
Steve
Steve Chapman FRMS Protrain for Consultancy and Training in Electron Microscopy +44 (0)7711 606967 web www.emcourses.com
-----Original Message----- X-from: dcristofori-at-unive.it [mailto:dcristofori-at-unive.it] Sent: 10 March 2017 15:07 To: protrain-at-emcourses.com
X-from: PSMnews-at-phillyscope.com
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Message: Joint PSM / Drexel Bossone Research Center Symposium on Advances in Microscopy
Wednesday, March 29 Please see the Events Registration page at http://phillyscope.com/events/ to register for the Symposium.
The Philadelphia Society for Microscopy will hold a joint all-day symposium on microscopy with the Drexel University Bossone Research Center in Philadelphia on March 29, 2017. Six talks will be presented on applications of microscopy in both physical and biological subjects. The keynote talks will be given by Jay Jerome of Vanderbilt University and Eric Wieschaus of Princeton University (1995 Nobel Prize in Physiology and Medicine). Jay Jerome will speak on A Microscopists View of Atherosclerosis and Cellular Cholesterol Metabolism. Eric Wieschaus will speak on Light Sheet Imaging of Global Tissue Flows in Living Drosophila Embryos. The entire program is available on http://phillyscope.com/symposium-march-29th/
On-site Registration and check-in will begin at 8:00 am with the talks beginning at 8:40 am. Representatives of many microscopy related manufacturers will be on-hand to discuss latest developments in microscopes and accessories.
The registration fee is $25. This fee also includes PSM membership for 2017. Full time students can register for the reduced cost of $5.00. We have space for approximately 25 student posters. Students who present posters can attend the symposium at no cost. Please feel free to contact us using the Contact page (http://phillyscope.com/contact/) with further questions about posters.
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From glenshan72-at-gmail.com Tue Mar 14 16:34:56 2017 Return-Path: {glenshan72-at-gmail.com} Received: from gmail.com ([211.40.127.194]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v2ELYrV6003999 for {microscopylistserverarchive6-at-microscopy.com} ; Tue, 14 Mar 2017 16:34:55 -0500 Message-ID: {5A0DE88C.98553735-at-gmail.com}
Have not got any messages from the list for a while. Just testing.
==============================Original Headers============================== 1, 33 -- From Aleksandr.Mironov-at-manchester.ac.uk Wed Mar 15 05:04:48 2017 1, 33 -- Received: from probity.mcc.ac.uk (probity.mcc.ac.uk [130.88.200.94]) 1, 33 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v2FA4lU1031784 1, 33 -- for {Microscopy-at-microscopy.com} ; Wed, 15 Mar 2017 05:04:48 -0500 1, 33 -- Received: from asmtp1.its.manchester.ac.uk ([130.88.13.149]) 1, 33 -- by probity.mcc.ac.uk with esmtps (TLSv1:DHE-RSA-AES256-SHA:256) 1, 33 -- (Exim 4.85 (FreeBSD)) 1, 33 -- (envelope-from {Aleksandr.Mironov-at-manchester.ac.uk} ) 1, 33 -- id 1co5vK-000Mfw-Mh 1, 33 -- for Microscopy-at-microscopy.com; Wed, 15 Mar 2017 10:12:54 +0000 1, 33 -- Received: from l-u-mid1527am.it.manchester.ac.uk ([130.88.210.224]:55477) 1, 33 -- by asmtp1.its.manchester.ac.uk with esmtpsa (TLSv1.2:ECDHE-RSA-AES128-GCM-SHA256:128) 1, 33 -- (Exim 4.88) 1, 33 -- (envelope-from {Aleksandr.Mironov-at-manchester.ac.uk} ) 1, 33 -- id 1co5vK-0001Hf-BR 1, 33 -- for Microscopy-at-microscopy.com; Wed, 15 Mar 2017 10:12:54 +0000 1, 33 -- To: Microscopy-at-microscopy.com 1, 33 -- From: Aleksandr Mironov {Aleksandr.Mironov-at-manchester.ac.uk} 1, 33 -- Subject: Test message 1, 33 -- Message-ID: {93c79c9a-1327-8a1f-50b3-36e3af19ae8f-at-manchester.ac.uk} 1, 33 -- Date: Wed, 15 Mar 2017 10:12:54 +0000 1, 33 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:45.0) Gecko/20100101 1, 33 -- Thunderbird/45.8.0 1, 33 -- MIME-Version: 1.0 1, 33 -- Content-Type: text/plain; charset=utf-8; format=flowed 1, 33 -- Content-Transfer-Encoding: 7bit 1, 33 -- X-Authenticated-Sender: Aleksandr Mironov from l-u-mid1527am.it.manchester.ac.uk [130.88.210.224]:55477 1, 33 -- X-Authenticated-From: Aleksandr.Mironov-at-manchester.ac.uk 1, 33 -- X-SA-Exim-Connect-IP: 130.88.13.149 1, 33 -- X-SA-Exim-Mail-From: Aleksandr.Mironov-at-manchester.ac.uk 1, 33 -- X-SA-Exim-Scanned: No (on probity.mcc.ac.uk); SAEximRunCond expanded to false 1, 33 -- X-Spam-Score: -5.0(?) 1, 33 -- X-Spam-Flag: NO ==============================End of - Headers==============================
From voraisai2-at-gmail.com Thu Mar 16 13:04:03 2017 Return-Path: {voraisai2-at-gmail.com} Received: from gmail.com (lync-mediation.hosted.chinatelecomglobal.com [203.14.188.88]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v2GI401w031709 for {microscopylistserverarchive6-at-microscopy.com} ; Thu, 16 Mar 2017 13:04:02 -0500 Message-ID: {91004185.C02BA2C8-at-gmail.com}
Does anybody have floppies from an old Leo 982 Gemini column? I just got one in the shop with the hard drive removed, and have to restore the on-board computer. (If you’ve got an old parts Leo 982 and have a spare drive with the software installed, and would be willing to part with it, that would work, too…)
Thank you,
Justin A. Kraft
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Email: pscallio-at-dal.ca Name: Pat Scallion
Organization: Institute for Research in Materials Dalhousie University
Title-Subject: [Filtered] S4700 column hot after a power outage
Message: Hi, I have seen odd behavior from the Hitachi S4700, after a power outage. When I arrived at work, the front instrument panel was showing only symbols, not numbers. Also, in addition to other usual things, the column was very hot, and the metal ticking, like what is heard during a bakeout.
I cannot get any image from the upper, TTL detector, and the beam is showing instability, with flashes of brightness and image jumping when in use. The Vext is slower to rise as well, and the IP3 starts at 1x10 -8, then rises to 1x10 -7 while the beam is on. The typical vacuum level for IP3 has been 1x10 -6.
I can send more information if specifics are needed.
Hope someone can help me.
Thanks, Pat Scallion
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From harremme142-at-gmail.com Sun Mar 19 15:03:37 2017 Return-Path: {harremme142-at-gmail.com} Received: from gmail.com (lync.hosted.chinatelecomglobal.com [203.14.188.89]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v2JK3YLe017146 for {microscopylistserverarchive6-at-microscopy.com} ; Sun, 19 Mar 2017 15:03:36 -0500 Message-ID: {FE6C826B.46F47528-at-gmail.com}
Good Morning Allan, I just purchased our second Leica UC7. These are great ultramicrotomes; very compact, easy to use and excellent lighting. I highly recommend them. Greg
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Wednesday, March 1, 2017 7:21 AM To: Hendricks, Gregory {Gregory.Hendricks-at-umassmed.edu}
X-from: allan.mitchell-at-otago.ac.nz
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Email: allan.mitchell-at-otago.ac.nz Name: Allan Mitchell
Organization: Otago centre for Electron Microscopy, University of Otago
Title-Subject: [Filtered] Ultramicrotomes and gridstainers
Message: We are in the process of trying to obtain funding to replace one of our ultramicrotomes (Ultracut E) and our aged LKB Ultrostainer. We are looking at - RMC Boeckeler ultramicrotme PT-PCZ and the RMC Boeckeler QG-3100 TEM Stainer, or
- Leica Ultracut EM UC7 ultramicrotome and Leica EM AC20 grid-stainer.
I would be keen to hear of peoples experiences with any of these instruments, positives /negatives.
Many thanks
Allan
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Dear Listers, here again to sum up the results of my little survey. It came out that keeping the LaB6 filament heated, even not at a full range, is quite the standard approach for this emitters.
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Email: szou-at-american.edu Name: Shouzhong Zou
Organization: American University
Title-Subject: [Filtered] Cost of adding an e-beam lithography to a JEOL SEM
Message: Hi Everyone. We are interested in adding an e-beam lithography to a JEOL JSM-IT100LA SEM. Do you have any ideas of how much this would cost? We just need a basic EBL to make electrical contact pads for graphene sheets. Thanks.
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Email: zhuoli-at-coh.org Name: Zhuo Li
Organization: City of Hope Beckman Research Institute
Title-Subject: [Filtered] Immuno-gold labeled cellular structures on the membrane?
Message: Dear Listers,
We did immunogold labeling on MDA-MB-231 breast cancer cells grown on cover glass to see if the antigen would appear on the cell surface. The cells were fixed in 4% paraformaldehyde and 0.1% glutaraldehyde in 0.1M cacodylate buffer pH7.4. Immunogold labeling was performed before critical point drying and sputter-coating with Au/Pd. Images were taken on a Zeiss Sigma FE-SEM. Besides some scattered signals, a lot of gold particles are localized in one area. An example is here: https://goo.gl/photos/YPXZrwzNAukfsHBw7 Could you tell what the structure it is? Also, there were a lot of gold particles on the background/cover glass? Could you suggest ways of eliminating them.
Thank you in advance!
Zhuo Li
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To evaluate the reliability of any immunolabelling it is required to at least check the negative controls: specimens incubated without primary antibody. Based on those results one can then look at background and what to do about it. If the negative control is clean, you are dealing with labelling based on binding of the primary. Pre-adsorption of the primary can help provide answers as to whether what you observe is specific or non-specific. If the negative control is not clean, the labelling is the result of interaction between gold conjugate and specimen. In that case incubation protocols and specimen conditioning are the first thing to look at.
Bakground, false positives can almost always be controlled. I will be happy to help with detailed suggestions if you would like, but since that might go into products and brands it would be better to do this off-list.
Cheers from sunny New Zealand,
Jan Leunissen Aurion
==============================Original Headers============================== 6, 26 -- From leunissen-at-aurion.nl Thu Mar 23 18:07:29 2017 6, 26 -- Received: from mx1.bbsrv.nl (mx1.bbsrv.nl [84.241.139.140]) 6, 26 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v2NN7TUk024008 6, 26 -- for {microscopy-at-microscopy.com} ; Thu, 23 Mar 2017 18:07:29 -0500 6, 26 -- Received: from localhost (localhost [127.0.0.1]) 6, 26 -- by mx1.bbsrv.nl (Postfix) with ESMTP id 691AF3104; 6, 26 -- Fri, 24 Mar 2017 00:16:05 +0100 (CET) 6, 26 -- Received: from mx1.bbsrv.nl ([127.0.0.1]) 6, 26 -- by localhost (mx1d.bbint [127.0.0.1]) (amavisd-new, port 10024) 6, 26 -- with ESMTP id g5yciCoMvR31; Fri, 24 Mar 2017 00:16:05 +0100 (CET) 6, 26 -- Received: from [192.168.0.5] (122-61-123-118.jetstream.xtra.co.nz [122.61.123.118]) 6, 26 -- by mx1.bbsrv.nl (Postfix) with ESMTPSA id 2D83A30AF; 6, 26 -- Fri, 24 Mar 2017 00:16:03 +0100 (CET) 6, 26 -- Content-Type: text/plain; charset=us-ascii 6, 26 -- Mime-Version: 1.0 (Mac OS X Mail 9.3 \(3124\)) 6, 26 -- Subject: Re: [Microscopy] viaWWW:Immuno-gold labeled cellular structures on the membrane? 6, 26 -- From: Jan Leunissen {leunissen-at-aurion.nl} 6, 26 -- In-Reply-To: {201703232208.v2NM8ibN000943-at-microscopy.com} 6, 26 -- Date: Fri, 24 Mar 2017 12:15:57 +1300 6, 26 -- Cc: zhuoli-at-coh.org 6, 26 -- Message-Id: {651EF269-CDF0-4E8D-87B8-830D4D0D4B9A-at-aurion.nl} 6, 26 -- References: {201703232208.v2NM8ibN000943-at-microscopy.com} 6, 26 -- To: microscopy-at-microscopy.com 6, 26 -- X-Mailer: Apple Mail (2.3124) 6, 26 -- Content-Transfer-Encoding: 8bit 6, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v2NN7TUk024008 ==============================End of - Headers==============================
1. It seems, at least, two possibilities for your picture. One is the structure (with gold particles) could be extracellular matrix, which is usually 'sticky' for gold particles, or could contain the antigen. Another possibility is that it is a broken membrane - you are viewing the inner membrane, which might have more antigen, or being sticky. You may want to do a 'control' with full fixation (Glutaraldehyde + Osmium), which provide you a better resolution of the structure.
2. Aldehyde group, especially that from glutaraldehyde is very 'sticky' to antibodies. Since you are NOT viewing the internal structure, I strongly recommend to avoid using glutaraldehyde, not even 0.1%. It may explain, at least in part, the heavy labeling on your cover glass.
3. Blocking with BSA, and/or serum could reduce the non-specific background.
4. I recommend the sample be coated with carbon, instead of Au/Pd. You can then confirm the gold particles with a backscatter electron detector (here is a reference I published many years ago - Localization of myosin on sperm-cell-associated membranes of tobacco (Nicotiana tabacum L.) - https://link.springer.com/article/10.1007/BF01279082
5. As Jan suggested, control is critical!
Hope this helps and let me know if you need a PDF copy of the reference.
Good Luck,
Zhaojie
Zhaojie Zhang, Ph.D. Director, Jenkins Microscopy Facility University of Wyoming
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Thursday, March 23, 2017 4:14 PM To: Z.J. Zhang {ZZhang-at-uwyo.edu}
X-from: zhuoli-at-coh.org
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Email: zhuoli-at-coh.org Name: Zhuo Li
Organization: City of Hope Beckman Research Institute
Title-Subject: [Filtered] Immuno-gold labeled cellular structures on the membrane?
Message: Dear Listers,
We did immunogold labeling on MDA-MB-231 breast cancer cells grown on cover glass to see if the antigen would appear on the cell surface. The cells were fixed in 4% paraformaldehyde and 0.1% glutaraldehyde in 0.1M cacodylate buffer pH7.4. Immunogold labeling was performed before critical point drying and sputter-coating with Au/Pd. Images were taken on a Zeiss Sigma FE-SEM. Besides some scattered signals, a lot of gold particles are localized in one area. An example is here: https://goo.gl/photos/YPXZrwzNAukfsHBw7 Could you tell what the structure it is? Also, there were a lot of gold particles on the background/cover glass? Could you suggest ways of eliminating them.
Thank you in advance!
Zhuo Li
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Hi, The Institute of Low Temperature and Structure Research, Polish Academy of Sciences, Wroclaw, Poland has an immediate opening for an Electron Microscopy Technician.
The main responsibilities and duties are: operation and maintenance of FE-SEM (equipped with EDS and EBSD) and ancillary equipment; training and support for users in microscope operation and sample preparation and participation in scientific projects in the field of materials science.
Job requirements: Graduate (B.Sc is a minimum) in physics, material science, chemistry, or similar discipline; experience in operation of SEM and X-ray microanalysis (EDS) (knowledge of EBSD and TEM techniques will be an advantage); good written and oral skills in English.
To view full job posting please visit : http://www.intibs.pl/en/the-institute/news/687
Regards,
Leszek Kepinski
Institute of Low Temperature and Structure Research,
Polish Academy of Sciences,
P.O. Box 1410, 50-950 Wroclaw, Poland
e-mail: L.Kepinski-at-int.pan.wroc.pl
==============================Original Headers============================== 10, 18 -- From l.kepinski-at-int.pan.wroc.pl Fri Mar 24 14:22:48 2017 10, 18 -- Received: from mserv3.int.pan.wroc.pl (mailserver.int.pan.wroc.pl [156.17.85.6]) 10, 18 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v2OJMmT7009132 10, 18 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Mar 2017 14:22:48 -0500 10, 18 -- Received: from poczta.intibs.pl (poczta.int.pan.wroc.pl [156.17.85.10]) 10, 18 -- by mserv3.int.pan.wroc.pl (Postfix) with ESMTPA id 951A9664F7 10, 18 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Mar 2017 20:31:28 +0100 (CET) 10, 18 -- MIME-Version: 1.0 10, 18 -- Content-Type: text/plain; charset=US-ASCII; 10, 18 -- format=flowed 10, 18 -- Content-Transfer-Encoding: 7bit 10, 18 -- Date: Fri, 24 Mar 2017 20:31:28 +0100 10, 18 -- From: "l.kepinski" {l.kepinski-at-int.pan.wroc.pl} 10, 18 -- To: Microscopy-at-microscopy.com 10, 18 -- Subject: Open position SEM 10, 18 -- Message-ID: {6738871a8992cd64e1c03359823477e7-at-int.pan.wroc.pl} 10, 18 -- X-Sender: l.kepinski-at-int.pan.wroc.pl 10, 18 -- User-Agent: Roundcube Webmail/1.0.9 ==============================End of - Headers==============================
The postdoctoral associate is expected to develop and perform TEM characterization of assembly of designed bio-molecular arrays. The research will involve the development of new approaches for cryoEM, cryoET, cryoSTEM, liquidEM characterization of an integration of proteins, peptides and DNA into targeted structures with multiscale organization, and analysis of their structure and assembly processes. The work will be conducted in the Electron Microscopy group in the Center for Functional Nanomaterials which houses five state-of-the-art scanning/ transmission electron microscopes. The majority of the work will be conducted on a FEI Environmental Titan equipped with a K2 direct-electron detector, a FEI Talos F200X, and an aberration-corrected dedicated STEM, first two of which have cryo-transfer, liquid-flow, low-dose, and automated data acquisition capabilities. The successful implementation of the work requires a close interaction with scientists working on bio-inspired self-assembly and advanced characterization methods using x-ray scattering.
Review of applications begins immediately. Applications will be accepted until the position is filled. Research will be under the direction of two well-known electron microscopists at the CFN in close collaboration with PIs and postdocs from the Soft/Bio Group, the Biology Department, and NSLSII.
Required Knowledge, Skills and Abilities:
1. Ph.D. in Physics, Materials Science, Chemistry or Biology 2. Solid background in Transmission Electron Microscopy with rigorous Ph.D. training in a cryoEM or TEM group. 3. Skills in cryoEM/TEM structural characterization at the atomic scale.
Preferred Knowledge, Skills, and Abilities:
Cryo-electron microscopy, single-particle and tomography methods, hands-on experience with TEM sample preparation, such as cryo-plunging and ultramicrotomy
Other Information:
BNL policy states that Research Associate appointments may be made to those who have received their doctoral degrees within the past five years.
The EM facility in the CFN includes 5 transmission electron microscopes, 1 dual-beam FIB, and a collection of specialized holders including liquid and gas flow holders, liquid electrochemical holders, heating holders (single-tilt, double-tilt, and high tilt), cryo-transfer holders (single tilt, double tilt, and high tilt), and nanoindentation holders (single and double tilt).
Interested candidates can apply online by clicking on the link below:
Best Regards, Huolin Xin, Ph.D. Associate Scientist Center for Functional Nanomaterials at Brookhaven National Laboratory and Adjunct Assistant Professor Department of Materials Science and Engineering SUNY Stony Brook University https://sites.google.com/site/xinhuolin/ Email: hxin-at-bnl.gov Office: 631-344-4350
==============================Original Headers============================== 13, 37 -- From xinhuolin-at-gmail.com Fri Mar 24 22:27:47 2017 13, 37 -- Received: from mail-oi0-f52.google.com (mail-oi0-f52.google.com [209.85.218.52]) 13, 37 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v2P3Rlb8010205 13, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Mar 2017 22:27:47 -0500 13, 37 -- Received: by mail-oi0-f52.google.com with SMTP id r203so3475200oib.3 13, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Mar 2017 20:36:31 -0700 (PDT) 13, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 13, 37 -- d=gmail.com; s=20161025; 13, 37 -- h=mime-version:from:date:message-id:subject:to; 13, 37 -- bh=zkfoCNQDFPAbz8HZklpxeEPTFMOtPww2bfwFhkde9TE=; 13, 37 -- b=Uw6xeFR+5MV7Ztr8R0k4ZzxGEwwPGvYcU+oLFWbIotlg1gPrss4CIUz1GIDtxz98Fe 13, 37 -- tWB8ALixRDLxnKR9GsfIogbivI0nXKV168jJlZNutOU63MkPqnt7uEsw8MMpXrMhU42C 13, 37 -- wDHpdBok51F7lZO9aqc38DhZPhU4KZCogkrzW/MhQjQ5dhyZYfF+48HxX1YwcLmGhz91 13, 37 -- EVyf+S2L/dCtMBdz33HX8XCJWX+FJOC8zJ4n8LsFzNc1HR+JRaTOBnBYChzsxCiisKQk 13, 37 -- mqTxoiwbcMwybFW/n2JXhaxasNi1Rww06XJ5O+VWRCnWV/JHVJOYyrRMBa83rIcuzCwd 13, 37 -- bJuQ== 13, 37 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 13, 37 -- d=1e100.net; s=20161025; 13, 37 -- h=x-gm-message-state:mime-version:from:date:message-id:subject:to; 13, 37 -- bh=zkfoCNQDFPAbz8HZklpxeEPTFMOtPww2bfwFhkde9TE=; 13, 37 -- b=QWdWB1SUtNO6L8BDqoEsqUUslhY87RMIhtwuHt5/NTxyL1KvhnoyGF/vvQZw8Mu/0A 13, 37 -- ODkUQMaJaRsfCKS1P7vBrB+ZBR0HL09h0t46KwMp5GoiddUFFdgC+QLXBqPbQQuAJIHE 13, 37 -- IPy8XKc13qf4bpdIsprAOr8pu2656qL3SgKAeLpO4DFgg3b4vc7DGJRw2cBukTkANBWZ 13, 37 -- aDKiKT6d2DkIaMWpXGFmchN9A4klnzdr+p4u2TXQNuKWZtwxnaczFdo/SMjwXp+wTpWC 13, 37 -- e4GcgUhiGKzVNA2EjydIAYanjcnDGV59JofjvM3t6J/Kx/xqPq4WwM6AZ8Fzkf+48uT1 13, 37 -- u4UQ== 13, 37 -- X-Gm-Message-State: AFeK/H11B7DeqOP1jcS7hODpEEvImjRVWpSLiWwBvUl9wQF530dbostZTp1AsZlPap3/Qu7m9Ei689SIEbOcoA== 13, 37 -- X-Received: by 10.202.79.13 with SMTP id d13mr3424oib.1.1490412990497; Fri, 24 13, 37 -- Mar 2017 20:36:30 -0700 (PDT) 13, 37 -- MIME-Version: 1.0 13, 37 -- Received: by 10.74.14.146 with HTTP; Fri, 24 Mar 2017 20:36:10 -0700 (PDT) 13, 37 -- From: Huolin Xin {xinhuolin-at-gmail.com} 13, 37 -- Date: Fri, 24 Mar 2017 22:36:10 -0500 13, 37 -- Message-ID: {CALG6ZvGPWRviXsQ3nE-XBZQ64eX7Ra6jx5Q_XdGXTwXRzf0NsQ-at-mail.gmail.com} 13, 37 -- Subject: CryoEM/TEM Postdoc Position at Brookhaven National Laboratory 13, 37 -- To: Microscopy-at-microscopy.com 13, 37 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
I am looking for a postdoc in the area of STEM and electron tomography. The postdoc will have the opportunity to work with a suite of tools in the Center for Functional Nanomaterials at Brookhaven National Laboratory, including an aberration-corrected FEI Environmental Titan equipped with a K2 direct-electron detector, a FEI Talos F200X equipped with X-FEG, Super-X, and an Enfinium, an aberration-corrected cold-FEG dedicated STEM, and a Nion HERMES (located in Rutgers), first two of which have analytical tomography, cryoET, cryoEM, and liquidEM capabilities. In total, the facility includes 5 transmission electron microscopes, 1 dual-beam FIB, and a collection of specialized holders including liquid and gas flow holders, liquid electrochemical holders, heating holders (single, double, and high tilt), cryogenic holders (single tilt, double tilt, and high tilt), and nanoindentation holders (single and double tilt).
Interested applicants should send a CV, and a one-paragraph description of his/her research/education background. Review of applications begins immediately. Applications will be accepted until the position is filled.
Best Regards, Huolin Xin, Ph.D. Associate Scientist Center for Functional Nanomaterials at Brookhaven National Laboratory and Adjunct Assistant Professor Department of Materials Science and Engineering SUNY Stony Brook University https://sites.google.com/site/xinhuolin/ Email: xinhuolin-at-gmail.com Office: 631-344-4350
==============================Original Headers============================== 4, 37 -- From xinhuolin-at-gmail.com Fri Mar 24 22:32:21 2017 4, 37 -- Received: from mail-ot0-f181.google.com (mail-ot0-f181.google.com [74.125.82.181]) 4, 37 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v2P3WLAK014342 4, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Mar 2017 22:32:21 -0500 4, 37 -- Received: by mail-ot0-f181.google.com with SMTP id a5so4553619oth.1 4, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Mar 2017 20:41:05 -0700 (PDT) 4, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 37 -- d=gmail.com; s=20161025; 4, 37 -- h=mime-version:from:date:message-id:subject:to; 4, 37 -- bh=5Xcxr3NbGwIIN2IbtErTrqEqsNT7Cpoe2qa+2f7w2oI=; 4, 37 -- b=T1MKURD9YeQQjF5bO9Q8+ZAdgMLD9Bo27jeEDoJdLPtacoIxIHpp/hpesfRMBS5vJc 4, 37 -- ROHz23TaFVASZjp+b2/YQVLlXMms6r1PrFrWFUiRuKd9fXoBD48HVsAYfr2xeKLlfND1 4, 37 -- pZ0ls1Y81GNAgD15h/uJGAM38lVjnfx19VuO12hS9aCRfZR7EsD4KgMWrBOb/4QE+sEy 4, 37 -- ySBsjG3EoQC/Pdl+K/x7787ZyhMsLoRc5hxbinKs56R1AneBCFzl+vH0bKDXheeoxvsW 4, 37 -- KV39R/2fgJUz0XibAhOAis69/LrblP/tTkq3ys1JKiHHL8gqnGZx4Dn5rjAh7Da3G5XG 4, 37 -- A87Q== 4, 37 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 37 -- d=1e100.net; s=20161025; 4, 37 -- h=x-gm-message-state:mime-version:from:date:message-id:subject:to; 4, 37 -- bh=5Xcxr3NbGwIIN2IbtErTrqEqsNT7Cpoe2qa+2f7w2oI=; 4, 37 -- b=lGneSSPa9n69oaDgYYXKVeO7B4iQ+bhlhRi/0/6w7uLnf34GxTsV6jhVtunL+zVf3H 4, 37 -- knvUX+3XUkBW/myt/7Oa96g1SdzWIjo7VicOx7KCIlINXkrzd7u+oLNT8TSsNxttz5Qh 4, 37 -- 57n18RnDSdQmO5Q84u6wyjp67ydltElkBfEOUamEPpRG+6Q4s8gESKFjDUVJDFJs/H7r 4, 37 -- RqItgEkM/wCMf68LNwVdeetYS9hVSBgWBVLaZTZBuL5ER0T+anrAG6uZeBdjq8CpXtaQ 4, 37 -- PU4F7bBNuc9RqqK7Cbq3fMJ/5Il4tiMEDa/aSyAX2jxNjsV3vCKBU5n92xt6t5+v5e/3 4, 37 -- 6XHA== 4, 37 -- X-Gm-Message-State: AFeK/H1OEhs4p8fcqT7AekKGWYmxYldJNCE5j16RYLtSabJSUxHqxdtS7qPKOWgJH0sJ45cjaSqPiLNR8Dr91w== 4, 37 -- X-Received: by 10.157.5.1 with SMTP id 1mr6048067otw.162.1490413264651; Fri, 4, 37 -- 24 Mar 2017 20:41:04 -0700 (PDT) 4, 37 -- MIME-Version: 1.0 4, 37 -- Received: by 10.74.14.146 with HTTP; Fri, 24 Mar 2017 20:40:44 -0700 (PDT) 4, 37 -- From: Huolin Xin {xinhuolin-at-gmail.com} 4, 37 -- Date: Fri, 24 Mar 2017 22:40:44 -0500 4, 37 -- Message-ID: {CALG6ZvEosrqdrkGrqa76Zm1eZNRAf3bFPmS7cdZHQtk6NHY45Q-at-mail.gmail.com} 4, 37 -- Subject: STEM/Tomography Postdoc Position at Brookhaven National Laboratory 4, 37 -- To: Microscopy-at-microscopy.com 4, 37 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both zhuoli-at-coh.org as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: zhuoli-at-coh.org Name: Zhuo Li
Organization: City of Hope Beckman Research Institute
Title-Subject: [Filtered] Immuno-gold labeled cellular structures on the membrane?
Message: Dear Listers,
We did immunogold labeling on MDA-MB-231 breast cancer cells grown on cover glass to see if the antigen would appear on the cell surface. The cells were fixed in 4% paraformaldehyde and 0.1% glutaraldehyde in 0.1M cacodylate buffer pH7.4. Immunogold labeling was performed before critical point drying and sputter-coating with Au/Pd. Images were taken on a Zeiss Sigma FE-SEM. Besides some scattered signals, a lot of gold particles are localized in one area. An example is here: https://goo.gl/photos/YPXZrwzNAukfsHBw7 Could you tell what the structure it is? Also, there were a lot of gold particles on the background/cover glass? Could you suggest ways of eliminating them.
Thank you in advance!
Zhuo Li
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Title-Subject: [Filtered] The Current Level in ET Detectors After the PRE-AMP stage
Message: Hi,
I have recently started studying SEMs and was looking into the Detector Current value after the Pre-Amplifier stage and before converting it to Digital Voltage.
Can anyone please help me with that
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==============================Original Headers============================== 14, 52 -- From microscopy.listserver-at-gmail.com Mon Mar 27 11:45:10 2017 14, 52 -- Received: from mail-it0-f45.google.com (mail-it0-f45.google.com [209.85.214.45]) 14, 52 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v2RGjAdt007089 14, 52 -- for {microscopy-at-microscopy.com} ; Mon, 27 Mar 2017 11:45:10 -0500 14, 52 -- Received: by mail-it0-f45.google.com with SMTP id y18so81754068itc.0 14, 52 -- for {microscopy-at-microscopy.com} ; Mon, 27 Mar 2017 09:54:03 -0700 (PDT) 14, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 14, 52 -- d=gmail.com; s=20161025; 14, 52 -- h=from:subject:references:reply-to:to:message-id:date:user-agent 14, 52 -- :mime-version:in-reply-to:content-transfer-encoding; 14, 52 -- bh=n/9UvBCmNfWzydbj3xBvdKkOYjhzoBiItpKHtIQlqDM=; 14, 52 -- b=C+8QUYzmFZr07ntri7ALxPngIMWslPDby4Gr1u34BnoujBwqhlW1hs80mQkzYBNRT7 14, 52 -- mXXUe0ysZ2fv18LVycD1+Cv3EKhvh+ELha3SadpTrpnwYMD3H6fdiz8ToNEf4L6B3m0c 14, 52 -- uev/xFHVfQtx6Wo9DZIi0U5ibkQiiNm1agrM+RNXzRd7U/mFYhzBJ3HJJOieE8EJFJV3 14, 52 -- h8UEbZ4mCoHGpxyhTxiqGl9vzNg4OPahJ6JuEVy0gBJy//eKd7uR9y31J21bP2APjZbX 14, 52 -- o9aJRU4um+K5iayyeHWlBwOApGzFq+977B8bbXJ5Kq/G3ZJ5Y/Ete75hrdJf4Seg1AkP 14, 52 -- 7X5Q== 14, 52 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 14, 52 -- d=1e100.net; s=20161025; 14, 52 -- h=x-gm-message-state:from:subject:references:reply-to:to:message-id 14, 52 -- :date:user-agent:mime-version:in-reply-to:content-transfer-encoding; 14, 52 -- bh=n/9UvBCmNfWzydbj3xBvdKkOYjhzoBiItpKHtIQlqDM=; 14, 52 -- b=AWm4fGkKt3WBUckliylzSsZFT0UudOi+Q09GGCagfg8IE5fTCkteVkLDkZn2macM1r 14, 52 -- gFTwrPziTurIMIh7kEbOtZU10mMQ1qzMt9r4fZZnA3WzVzrWGH0yzrfIwwTaUMa2GCis 14, 52 -- 0tsopt6bo4L3PNsLjGqsVt+Yy9fiwu3lHotcIHfh7ZwEfOnfMPshkx33sV/IeDlM8sLK 14, 52 -- HlBtmFl8taoPWrQJxNEaY1byrIgi40eel/pCV87tFAOGEuU/foxLO2oEzmgc533sbyHg 14, 52 -- pTYBy2JeLiOAAn+vgj99APxcU55lV6b1n5q0RD7xodbqsPmEZ87bpTHblE2G8+u3Udvu 14, 52 -- QN0A== 14, 52 -- X-Gm-Message-State: AFeK/H0CS1t+a2cZCNOZ7a7ejstozfnRzfcJirM/sPnQ3INP0Qrp/Bav1IgBGPh2lTeF1g== 14, 52 -- X-Received: by 10.107.53.88 with SMTP id c85mr24062931ioa.185.1490633642497; 14, 52 -- Mon, 27 Mar 2017 09:54:02 -0700 (PDT) 14, 52 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:590a:6045:62c8:ca79]) 14, 52 -- by smtp.googlemail.com with ESMTPSA id f127sm64986ite.14.2017.03.27.09.54.01 14, 52 -- for {microscopy-at-microscopy.com} 14, 52 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 14, 52 -- Mon, 27 Mar 2017 09:54:02 -0700 (PDT) 14, 52 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 14, 52 -- X-Google-Original-From: MicroscopyListserver-NoReply 14, 52 -- {microscopylistserver-noreply-at-microscopy.com} 14, 52 -- Subject: viaWWW: The Current Level in ET Detectors After the PRE-AMP stage 14, 52 -- References: {201703271243.v2RChBMN030943-at-microscopy.com} 14, 52 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 14, 52 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 52 -- X-Forwarded-Message-Id: {201703271243.v2RChBMN030943-at-microscopy.com} 14, 52 -- Message-ID: {87e548fa-bc50-a24d-93a5-08c0b8698020-at-microscopy.com} 14, 52 -- Date: Mon, 27 Mar 2017 11:54:01 -0500 14, 52 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:45.0) 14, 52 -- Gecko/20100101 Thunderbird/45.8.0 14, 52 -- MIME-Version: 1.0 14, 52 -- In-Reply-To: {201703271243.v2RChBMN030943-at-microscopy.com} 14, 52 -- Content-Type: text/plain; charset=windows-1252; format=flowed 14, 52 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: jotham-at-uchicago.edu Name: Joe Austin
Organization: The University of Chicago
Title-Subject: [Filtered] EM Technical Director position at University of Chicago
Message: Dear Colleagues,
I would like to call your attention to a Technical Director position in the Advanced Electron Microscopy facility at The University of Chicago (Requisition Number 102322).
For more information about the position and to apply please visit this link: https://jobopportunities.uchicago.edu/applicants/jsp/shared/position/JobDetails_css.jsp?postingId=670183
This position will be open until filled and will be reviewing applications on an ongoing basis. If you have questions about the position please email Joe Austin at jotham-at-uchicago.edu. If you have any difficulty uploading your application or any questions, please email Manuel Carrasquillo at mcarrasquillo-at-bsd.uchicago.edu
The University of Chicago is an Affirmative Action/Equal Opportunity/Disabled/Veterans Employer and does not discriminate on the basis of race, color, religion, sex, sexual orientation, gender identity, national or ethnic origin, age, status as an individual with a disability, protected veteran status, genetic information, or other protected classes under the law. For additional information please see the University's Notice of Nondiscrimination. Staff Job seekers in need of a reasonable accommodation to complete the application process should call 773-834-1841 or email talentacquisition-at-uchicago.edu with their request.
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Email: jpshield-at-uga.edu Name: John P Shields
Organization: University of Georgia
Title-Subject: [Filtered] Cathodoluminescence in SEM
Message: We are in the process of adding CL to our FE-SEM. I am not familiar enough with the detectors to know if this is a pain or a positive.
Some questions would be: What is the average lifespan (if there is one) for these detectors? What kind of trouble can you get into with them? Are there any conflicts with other detectors (real or imagined)? How easy is it to use one? Any information that cannot be easily googled would be appreciated.
John S
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Message: Does anybody out there have any experience with the Gatan multiple(3)grid cryo holder? We are specifically interested in the grid locking system.
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Email: Hasan.Ali-at-angstrom.uu.se Name: Hasan Ali
Organization: Uppsala University
Title-Subject: [Filtered] Free Lens Control On JEOL2000FX
Message: Hi,
We have assembled a Gatan energy filter GIF2002 under an old JEOL2000FX. As the GIF doesn't have any communication with the microscope, we don't have a magnification reduction in the microscope when using the GIF and the image/diffraction pattern on the viewing screen is magnified about 19X on the GIF CCD. I want to do angular resolved EELS, but even at the lowest automated camera length (100mm) in the microscope, I cannot get the two beams simultaneously on the CCD for my sample (the collection angle is too low). So I need to go to lower camera lengths using "Free Lens Control" available in the microscpe. I tried Free lens control and obtained the lower camera lengths by changing the strength of intermediate lenses, but when I switch back to image mode, the settings there are changes too and the sample is no more on eucentric height. I don't understand exactly now which lens should I tune in combination with intermediate lenses. Does anyone have the experience of operating the microscope in Free lens control who could suggest me something to follow? Also if someone have the manual to operate the JEOL2000FX in Free Lens Control, that will be very helpful for me. I didn't get information about this in the manuals which I have. Thanks
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From fritschemichael755-at-gmail.com Fri Mar 31 04:20:40 2017 Return-Path: {fritschemichael755-at-gmail.com} Received: from gmail.com (mail.dongnamdc.com [222.97.39.100] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v2V9Kb4Y010736 for {microscopylistserverarchive6-at-microscopy.com} ; Fri, 31 Mar 2017 04:20:39 -0500 Message-ID: {6016F1BB.CABAF2A4-at-gmail.com}
X-from: kmoore-at-vetcor.com
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Email: kmoore-at-vetcor.com Name: Kasandra Moore
Organization: Geist Station Animal Hospital
Title-Subject: [Filtered] Maintenance Microscope Message: We have a Swift Instrument international SA M3200 microscope. The images are dirty. We think the microscope needs to be cleaned. Can someone provide this service in the Indianapolis area?
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From westelle638-at-gmail.com Sun Apr 2 14:48:15 2017 Return-Path: {westelle638-at-gmail.com} Received: from gmail.com ([221.160.34.117]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v32JmCIC004729 for {microscopylistserverarchive6-at-microscopy.com} ; Sun, 2 Apr 2017 14:48:14 -0500 Message-ID: {AB55A17E.C16BAE9B-at-gmail.com}
The 53rd anniversary meeting of the Southeastern Microscopy Society will be at the Holiday Inn in Athens, GA, on May 24-26, 2017! There is an exciting program planned; Mike Marko of the Wadsworth Center and past president of MSA will be our Invited Speaker. In addition, there will be contributed talks from the members and the Ruska Award student presentations. (Encourage your students to compete in the Ruska Award competition!) A Vendor Social will be held on Wednesday evening, a Banquet on Thursday evening, and a Business Breakfast on Friday morning.
Information is available at http://southeasternmicroscopy.org/2017-2/
Half-day Workshops on Wednesday will include:
RMC and an Array ultramicrotome (at Holiday Inn) FEI and FE-SEM STEM and analysis (at GEM on UGA campus) Negative staining with Sara Miller and Mary Ard (at GEM on UGA campus) Protochips with wet/dry TEM sample holder. (at GEM on UGA campus) Possible Confocal workshop with Zeiss (at the BioImaging Center at UGA)
The abstract deadline is April 14. Please encourage your students to apply for the Ruska Award! http://southeasternmicroscopy.org/ruska-award-student-competition/
The SEMS website is ready for your registration, available at http://southeasternmicroscopy.org/2017-2/ . You can also get to the hotel reservation webpage by clicking on the link on the meeting website.
We hope to see you in Athens!
Terri Bruce (terri-at-clemson.edu), Program Chair John Shields (johnshields-at-gmail.com) and Mary Ard (maryard-at-uga.edu), Local Arrangements Committee
==============================Original Headers============================== 3, 32 -- From ALawrence-at-i2at.msstate.edu Mon Apr 3 12:24:22 2017 3, 32 -- Received: from catalpa.its.msstate.edu (catalpa.its.msstate.edu [130.18.2.119]) 3, 32 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v33HOMEb020873 3, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 3 Apr 2017 12:24:22 -0500 3, 32 -- Received: from mail01.ad.msstate.edu (mail01.ad.msstate.edu [130.18.230.60]) 3, 32 -- by catalpa.its.msstate.edu (8.13.8/8.13.8) with ESMTP id v33HXet3001828 3, 32 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=FAIL) 3, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 3 Apr 2017 12:33:40 -0500 3, 32 -- X-Sender: {} 3, 32 -- Received: from MAIL02.ad.msstate.edu (2620:0:1a30:1230::61) by 3, 32 -- mail01.ad.msstate.edu (2620:0:1a30:1230:85cd:146c:55d4:e430) with Microsoft 3, 32 -- SMTP Server (TLS) id 15.0.1236.3; Mon, 3 Apr 2017 12:33:40 -0500 3, 32 -- Received: from MAIL02.ad.msstate.edu ([fe80::7846:3039:9492:24b0]) by 3, 32 -- mail02.ad.msstate.edu ([fe80::7846:3039:9492:24b0%13]) with mapi id 3, 32 -- 15.00.1236.000; Mon, 3 Apr 2017 12:33:40 -0500 3, 32 -- From: "Lawrence, Amanda" {ALawrence-at-i2at.msstate.edu} 3, 32 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 3, 32 -- Subject: Southeastern Microscopy Meeting, May 24-26 3, 32 -- Thread-Topic: Southeastern Microscopy Meeting, May 24-26 3, 32 -- Thread-Index: AdKsoEJfrTuc/oYFTX6+DsRq0qNfRQ== 3, 32 -- Date: Mon, 3 Apr 2017 17:33:39 +0000 3, 32 -- Message-ID: {80871a69b31449899ee9cb1a087859b3-at-mail02.ad.msstate.edu} 3, 32 -- Accept-Language: en-US 3, 32 -- Content-Language: en-US 3, 32 -- X-MS-Has-Attach: 3, 32 -- X-MS-TNEF-Correlator: 3, 32 -- x-ms-exchange-transport-fromentityheader: Hosted 3, 32 -- x-originating-ip: [130.18.230.93] 3, 32 -- Content-Type: text/plain; charset="iso-8859-1" 3, 32 -- MIME-Version: 1.0 3, 32 -- Content-Transfer-Encoding: 8bit 3, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v33HOMEb020873 ==============================End of - Headers==============================
From operacyjny-at-sezambiuro.pl Mon Apr 3 15:07:31 2017 Return-Path: {operacyjny-at-sezambiuro.pl} Received: from 3580639525.smtp.ogicom.net (3580639525.smtp.ogicom.net [213.108.57.37]) by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v33K7T3k017946; Mon, 3 Apr 2017 15:07:30 -0500 Received: from localhost (localhost.localdomain [127.0.0.1]) by s28-mail.ogicom.net (Postfix) with ESMTP id 598B3A491FD8; Mon, 3 Apr 2017 22:16:47 +0200 (CEST) X-Virus-Scanned: Debian amavisd-new at s28-mail.ogicom.net Received: from s28-mail.ogicom.net ([93.157.100.47]) by localhost (s28-mail.ogicom.net [127.0.0.1]) (amavisd-new, port 10026) with ESMTP id gu4MtyxBOKBp; Mon, 3 Apr 2017 22:16:45 +0200 (CEST) Received: from WS5400D899.PF.local (rrcs-24-172-6-178.midsouth.biz.rr.com [24.172.6.178]) (Authenticated sender: operacyjny.bosezam) by s28-mail.ogicom.net (Postfix) with ESMTPA id 0F014A481449; Mon, 3 Apr 2017 22:16:37 +0200 (CEST) Content-Type: multipart/alternative; boundary="===============1890162325==" MIME-Version: 1.0
X-from: andre.dufresne-at-umanitoba.ca
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Message: Hy everyone, I'm looking for a water chiller for a TEM and SEM (H-7000) The unit I'm looking for is a Haskris R075 or equivalent. Options: Water cooled
Message me directly if you have any questions or know the availability of such a unit. Merci /Thank you.
AD
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Title-Subject: [Filtered] The Aurion Immuno Gold Silver Staining Workshop
Message: Three days of hands-on training for students, researchers, and microscopists who want to learn the most up to date theory and practice in Immuno Gold labeling. Details: Wednesday - Friday May 9-12, 2017 8:00 a.m. - 4:30 p.m. EMS Microscopy Academy Hatfield, Pennsylvania, USA
Instructors: Peter Van De Plas, Aurion, The Netherlands Michael Kostrna, Director, EMS Microscopy Academy Al Coritz, Technical Director, Electron Microscopy Sciences
Class size is limited! For more details, including online registration, visit: http://www.emsdiasum.com/microscopy/academy/courses/immunogold.aspx Login Host: 73.165.127.32 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
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Email: gtorraca-at-amgen.com Name: Gianni Torraca
Organization: MSA
Title-Subject: [Filtered] Freeze fracture TEM of biologics wanted - service work
Message: Good Day,
I am looking for a lab that can perform freeze fracture analysis of a biologic solution. Kinds regards Gianni Torraca Sr. Scientist Amgen Inc.
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From kimgarr459-at-gmail.com Fri Apr 7 13:02:06 2017 Return-Path: {kimgarr459-at-gmail.com} Received: from gmail.com (59-100-3-189.syd.static-ipl.aapt.com.au [59.100.3.189]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v37I228n024525 for {microscopylistserverarchive6-at-microscopy.com} ; Fri, 7 Apr 2017 13:02:04 -0500 Message-ID: {A014D037.891BAFC7-at-gmail.com}
Bernard J. "Bernie” Kestel the 1994 Microscopy Society of America’s Technologist of the year passed away on April 7.
Bernie was an engineering specialist microscopy technologist for 44 years at Argonne National Laboratory, retiring in 2002. Bernie published numerous articles in microscopy journals and was the recipient of the 1994 Technologist of the Year by the Microscopy Society of America, 1996 Pacesetter Award by Argonne National Laboratory and 1998 Outstanding Service Award by the University of Chicago.
His contributions to the community were in the area of specimen preparation, where he developed new techniques, preparated countless specimens and assisted/trained innumerable students, scientists and colleagues. A compendium of his methodology has been published as a scientific report and is freely available to anyone interested in electropolishing of materials.
Kestel B, (1986) Polishing Methods for Metallic and Ceramic Transmission Electron Microscopy Specimens, ANL-80-120/Rev.1 Report , available from NTIS DE89016686 Issue Number 199005 https://ntrl.ntis.gov/NTRL/dashboard/searchResults.xhtml?searchQuery=ANL-80-120
He was an artist in his field and could always be counted on to find a way to make that critical TEM sample, even when you only had 1small item to work with. He will be remembered here at Argonne and by those he helped in the community, myself included.
=========================================== Dr. Nestor J. Zaluzec Argonne National Laboratory Photon Sciences Division 9700 S. Cass Ave Bldg 212 / A-143 Argonne, Illinois 60439 USA Email: Zaluzec-at-aaem.amc.anl.gov
Tel: 530-NES-TORZ (530-637-8679) has Voice Mail Lab: 630-252-7901 Fax: 630-252-4798
Senior Scientist - Argonne National Laboratory Fellow of the Microscopy Society of America Senior Fellow the Computational Institute - University of Chicago E.P. Wigner Fellow - Oak Ridge National Laboratory Past President Microscopy Society of America Adjunct Professor of Physics - Northern Illinois University & the University of Illinois at Chicago Visiting Professor of Microscopy - Manchester University
*********************************************************************************** Forwarded from "Ask a Microscopist" Please remember that the person asking the question is likely not a member the listserver, and **any reply should go directly to the poster** as well as to the list. Using the "reply" function in your email does *not* send your answer to the person asking the question. Please copy their email address from their question. *********************************************************************************** Name: Richard Gursky School: St. Jude Children’s Research Hospital Grade/Education Level: Graduate Location: Memphis, TN US Email: richard.gursky-at-stjude.org
*********************************************************************************** Forwarded from "Ask a Microscopist" Please remember that the person asking the question is likely not a member the listserver, and **any reply should go directly to the poster** as well as to the list. Using the "reply" function in your email does *not* send your answer to the person asking the question. Please copy their email address from their question. *********************************************************************************** Listers,
This is mostly for those of you on the list who work as applications specialists or service engineers for microscope companies. Perhaps you can send some good advice to Brakel about how to get such a job. Please remember to respond directly to Brakel -- kbrakel91-at-gmail.com
I've already sent some ideas to the Brakel, but since I do not work as an applications person or a service engineer, some pointers from people who do would be more useful.
--------------- Philip Oshel Microscopy Society of America Ask a Microscopist www(dot)microscopy(dot)org/resources/ask(dot)cfm
Name: Kiralyn Brakel School: Texas A&M University Grade/Education Level: Graduate Location: College Station, TX US Email: kbrakel91-at-gmail.com
I'm trying to find the pre-amp pinout designations on an Oxford 7215 Link Pentafet EDS spectrometer. Oxford no longer supports this once highly touted piece of gear and have been unable to help me. Can anyone help with that?
Keeping legacy gear running is made much more difficult by lack of support. I have operated a small electron microprobe lab here in Seattle since 1984 and have useful gear from the entire history of x-ray microanalysis. I'm now using an old ARL SEMQ and a JEOL JXA 8600. I once had the ARL EMX-SM that had the actual moon rocks in it. My computers go all the way back to DEC PDP11s, though that's long gone. The SEMQ still uses MS 6.2. I have thought it might be good to have a used gear forum of some sort. Facebook page ? Wordpress blog ? Any thoughts on that too.
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Email: Linda.Davis-at-Vesuvius.com Name: Linda L Davis
Organization: Vesuvius USA
Title-Subject: [Filtered] celsian or barium feldspar specimens
Message: Hello All,
We have a new chemist in our R&D Analytical Group who needs to do some development work on analyzing barite and celsian using an ICP. I've found nice barite crystals to purchase, but now I need some celsian/barium feldspar. I cannot use eBay at all for work, so I have come to the listserve to see if anyone has any celsian we can purchase or have. I don't need a ton, but numerous crystals/grains to grind up for digestion and analysis. I would greatly appreciate any help.
Sincerely,
Linda L. Davis
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Organization: Oakland University Eye Research Institute
Title-Subject: [Filtered] Whole Cell Mount TEM - Carbon Coating
Message: When one does whole cell mount TEM nowadays on gold-Formvar grids, is it necessary to carbon-coat the samples after preparation (seeding, fixation, staining, dehydration and critical point drying by CO2) or is before the preparation sufficient?
Grids with cells will be stored in a dessicator filled with Drier-Rite.
Thanks, Vickie
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Name: Richard Gursky School: St. Jude Children’s Research Hospital Grade/Education Level: Graduate Location: Memphis, TN US Email: richard.gursky-at-stjude.org
Hello Shouzhong,
I would contact Joe Nabity to see if his Nanometer Pattern Generation System can be added to your 'scope. We use it on our FIB and are happy with the performance. You can find more information here: http://www.jcnabity.com/
I have no ties, financial or otherwise, to Joe or his company.
Thanks, Chris
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From fritschemichael755-at-gmail.com Sat Apr 15 10:08:54 2017 Return-Path: {fritschemichael755-at-gmail.com} Received: from gmail.com ([182.16.70.114]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v3FF8pL6028002 for {microscopylistserverarchive6-at-microscopy.com} ; Sat, 15 Apr 2017 10:08:53 -0500 Message-ID: {7ED2CF8A.D62BFD55-at-gmail.com}
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Email: mryan-at-cshl.edu Name: Marjorie Ryan
Organization: Cold Spring Harbor Laboratory
Title-Subject: [Filtered] Searching for an Electron Microscopy Technologist
Message: Cold Spring Harbor Laboratory seeks a highly motivated dedicated individual to work in a state-of-the-art Microscopy Shared Resource. The individual should have extensive practical expertise in biological sample preparation for transmission and scanning electron microscopy. Hands-on knowledge of confocal and widefield fluorescence microscopy would also be a plus. The candidate will help users design innovative experiments and they will carry out sample preparation and imaging as well as assist in data interpretation. Excellent verbal and written communication skills, ability to work with multiple users in a supporting role, and ability to work independently and proactively with limited supervision are essential. To Apply:
Interested individuals should send their resume, including a description of their practical expertise and the names and email addresses of 3 references to: Marjorie Ryan, Cold Spring Harbor Laboratory, One Bungtown Road, Cold Spring Harbor, New York 11724, email: mryan-at-cshl.edu Cold Spring Harbor Laboratory is a world-renowned research and educational institution recognized internationally for its excellence in ground-breaking research programs in cancer, neuroscience, plant biology, genomics, and bioinformatics and broad educational mission. For more information about CSHL, please visit us at www.cshl.edu CSHL is an EO/AA Employer. All qualified applicants will receive consideration for employment and will not be discriminated against on the basis of race, color, religion, sex, sexual orientation, gender identity, national origin, age, disability or protected veteran status. VEVRAA Federal Contractor
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From wandjoan59-at-gmail.com Mon Apr 17 04:05:20 2017 Return-Path: {wandjoan59-at-gmail.com} Received: from gmail.com ([182.16.74.250]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v3H95Ibu026977 for {microscopylistserverarchive6-at-microscopy.com} ; Mon, 17 Apr 2017 04:05:19 -0500 Message-ID: {0584B3E5.EBD7DE94-at-gmail.com}
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Email: ravi.thakkar369-at-gmail.com Name: Ravi
Organization: Ravi Thakkar
Title-Subject: [Filtered] Source of Standard zinc oxide and iron oxide sample for EDS reference.
Message: Hi All, One of the user for our EM facility is looking for Zinc Oxide and Iron Oxide as standard reference for EDS analysis. If any one can help. Thanks in advance. Login Host: 129.130.145.60 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
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We may need more information before we can answer the question well - at least, I would need more.
Is this EDS in conjunction with TEM or SEM? The form of the material would different for the two applications. - If TEM, I don't have much to suggest since I do SEM. It seems they would want a thin film or powder. - If SEM, they would probably want a homogenous, bulk sample. They can get bulk samples of iron oxide. Does it matter if it is Fe2O3 or Fe3O4? I am used to zinc oxide being a powder. It might be challenging to find it in bulk form.
What do they ultimately want to know? Do they want to know if their material matches? Do they want to quantify the oxide? Do they want to see if they have excess oxygen? I often find users coming in with too narrow a question. When I found out the true issue, there is usually much more freedom in suggesting a solution.
Why do they want the oxides? Most EDS systems will have standards built-in for the elements. They are often quite good. I wouldn't think that they need the oxides of the metals.
What form is their sample in, bulk, powder, film? If it is not flat, polished, thick material, then accurate quant will be out of the question.
Hopefully the answer is not simply "Because". I find it much easier to help someone who is forthcoming with information rather than one who is dead-set on a single course of action.
Regards, Warren Straszheim
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Monday, April 17, 2017 9:20 PM To: Straszheim, Warren E [BIOTC]
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Email: ravi.thakkar369-at-gmail.com Name: Ravi
Organization: Ravi Thakkar
Title-Subject: [Filtered] Source of Standard zinc oxide and iron oxide sample for EDS reference.
Message: Hi All, One of the user for our EM facility is looking for Zinc Oxide and Iron Oxide as standard reference for EDS analysis. If any one can help. Thanks in advance. Login Host: 129.130.145.60 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
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I'm struggling with Williams and Carter (2nd edition, I think) understanding HRTEM. I generally find their text approachable and easy to read, but not the HRTEM material, other than the math.
Is their a text or article that is more detailed? Outside of the Supposedly HRTEM sources that I have found have lengthy introductions to the basic, non-HR microscope, then brief descriptions of the math of HRTEM.
What's a good Read?
I'm a microscopist, with a B.S., so technical is okay, but I want a deep focus on HRTEM, theory and instrumentation.
Thanks!
Kleo (Kathleen) Pullin Moraga, CA 209-610-0555 kleopullin-at-email.arizona.edu https://www.linkedin.com/in/kleopullin https://twitter.com/resolvingdust
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We may need more information before we can answer the question well - at least, I would need more.
Is this EDS in conjunction with TEM or SEM? The form of the material would different for the two applications. - If TEM, I don't have much to suggest since I do SEM. It seems they would want a thin film or powder. - If SEM, they would probably want a homogenous, bulk sample. They can get bulk samples of iron oxide. Does it matter if it is Fe2O3 or Fe3O4? I am used to zinc oxide being a powder. It might be challenging to find it in bulk form.
What do they ultimately want to know? Do they want to know if their material matches? Do they want to quantify the oxide? Do they want to see if they have excess oxygen? I often find users coming in with too narrow a question. When I found out the true issue, there is usually much more freedom in suggesting a solution.
Why do they want the oxides? Most EDS systems will have standards built-in for the elements. They are often quite good. I wouldn't think that they need the oxides of the metals.
What form is their sample in, bulk, powder, film? If it is not flat, polished, thick material, then accurate quant will be out of the question.
Hopefully the answer is not simply "Because". I find it much easier to help someone who is forthcoming with information rather than one who is dead-set on a single course of action.
Regards, Warren Straszheim
-----Original Message----- X-from: microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} [mailto:microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} ] Sent: Monday, April 17, 2017 9:20 PM To: Straszheim, Warren E [BIOTC] Subject: [Microscopy] viaWWW:Source of Standard zinc oxide and iron oxide sample for EDS
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Email: ravi.thakkar369-at-gmail.com {mailto:ravi.thakkar369-at-gmail.com} Name: Ravi
Organization: Ravi Thakkar
Title-Subject: [Filtered] Source of Standard zinc oxide and iron oxide sample for EDS reference.
Message: Hi All, One of the user for our EM facility is looking for Zinc Oxide and Iron Oxide as standard reference for EDS analysis. If any one can help. Thanks in advance. Login Host: 129.130.145.60 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
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Email: michael.cammer-at-med.nyu.edu Name: Michael Cammer
Organization: NYULMC
Title-Subject: [Filtered] need 700-800 nm excitation for Zeiss AxioObserver
Message: We need to excite in the 700 to 800 nm range and the Zeiss HPX-120 lamp we have is filtered to block wavelengths above 660 nm. Rather than customize the filter inside the lamp house, we are looking for an alternative light source for the 700-800 nm range with a liquid light guide or fiber that we could swap for the Zeiss HPX-120 on the days we need to image infra-red.
Are there any simple light sources that fit this description that cost less than $3k? If not, how about ones that dont cost much more than this?
Thank you!
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I'd say it depends on how stable you want the line (frequency) to be.
CD-ROM lasers are 780nm, and super cheap thanks to economies of scale. Getting one coupled to the fiber of your choice would then be your challenge.
On Tue, Apr 18, 2017 at 6:08 PM, {microscopy.listserver-at-gmail.com} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: michael.cammer-at-med.nyu.edu } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } michael.cammer-at-med.nyu.edu as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: michael.cammer-at-med.nyu.edu Name: Michael Cammer } } Organization: NYULMC } } Title-Subject: [Filtered] need 700-800 nm excitation for Zeiss AxioObserver } } Message: We need to excite in the 700 to 800 nm range and the Zeiss HPX-120 lamp we have is filtered } to block wavelengths above 660 nm. Rather than customize the filter inside the lamp house, we are } looking for an alternative light source for the 700-800 nm range with a liquid light guide or fiber } that we could swap for the Zeiss HPX-120 on the days we need to image infra-red. } } Are there any simple light sources that fit this description that cost less than $3k? If not, how } about ones that don’t cost much more than this? } } Thank you!
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It should be possible to import .spx file into this software and then export the data in cvs format. Unfortunately I do not have any spx file at hand, but I have tested it on msa and spc files and it works well.
Best regards from Prague
Oldrich
-- Oldřich Benada Institute of Microbiology CAS, v.v.i. Laboratory of Molecular Structure Characterization Vídeňská 1083 142 20 Prague 4 Czech Republic
On Wed, 19 Apr 2017 06:59:11 -0500, oshel1pe-at-cmich.edu wrote : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } *********************************************************************************** } Forwarded from "Ask a Microscopist" } Please remember that the person asking the question is likely } not a member the listserver, and } **any reply should go directly to the poster** } as well as to the list. } Using the "reply" function in your email does *not* send your answer } to the person asking the question. } Please copy their email address from their question. } *********************************************************************************** } } -----Original Message----- } X-from: "AssociationManagement-at-microscopy.org" } {associationmanagement-at-microscopy.org} Date: Wednesday, 19April, } 2017 at 01:08 To: "AssociationManagement-at-microscopy.org" } {associationmanagement-at-microscopy.org} , Philip Oshel } {oshel1pe-at-cmich.edu} Subject: Ask a Microscopist } } Name:Anuja Bhalkikar } School:University of Nebraska Lincoln } Grade/Education Level:Graduate } Location:Lincoln, NE } Email:anuja.bhalkikar-at-huskers.unl.edu } Subject:Convert .spx data to .txt or .csvYour } Question:Hello, I recently used an FEI Tecnai Osiris S/TEM to obtain } EDS of my sample. I believe we have a Super-X EDX detection system in } conjunction with Bruker Esprit software. The data was unfortunately } saved as .spx. Is there any way I could convert it into .txt or .csv } format? 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==============================Original Headers============================== 8, 50 -- From benada-at-biomed.cas.cz Wed Apr 19 09:52:11 2017 8, 50 -- Received: from barracuda.biomed.cas.cz (barracuda.biomed.cas.cz [147.231.40.11]) 8, 50 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v3JEqAHD007526 8, 50 -- for {microscopy-at-microscopy.com} ; Wed, 19 Apr 2017 09:52:11 -0500 8, 50 -- X-ASG-Debug-ID: 1492614144-05011e10cb1abd10001-4CH8be 8, 50 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) by barracuda.biomed.cas.cz with ESMTP id sAGGIttF1QvZSiIg; Wed, 19 Apr 2017 17:02:24 +0200 (CEST) 8, 50 -- X-Barracuda-Envelope-From: benada-at-biomed.cas.cz 8, 50 -- X-Barracuda-Effective-Source-IP: mail2.biomed.cas.cz[147.231.40.32] 8, 50 -- X-Barracuda-Apparent-Source-IP: 147.231.40.32 8, 50 -- Received: from u117ob02 (nb170ph.mbu.cas.cz [147.231.44.133]) 8, 50 -- (using TLSv1.2 with cipher ECDHE-RSA-AES128-GCM-SHA256 (128/128 bits)) 8, 50 -- (No client certificate requested) 8, 50 -- by mail2.biomed.cas.cz (Postfix) with ESMTPSA id 57C90D015B6; 8, 50 -- Wed, 19 Apr 2017 17:02:23 +0200 (CEST) 8, 50 -- Date: Wed, 19 Apr 2017 17:02:22 +0200 8, 50 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 8, 50 -- To: anuja.bhalkikar-at-huskers.unl.edu, {microscopy-at-microscopy.com} 8, 50 -- Subject: Re: [Microscopy] FW: Ask a Microscopist- converting EDS files in 8, 50 -- .spx format 8, 50 -- Message-ID: {20170419170222.6b20441e-at-u117ob02} 8, 50 -- X-ASG-Orig-Subj: Re: [Microscopy] FW: Ask a Microscopist- converting EDS files in 8, 50 -- .spx format 8, 50 -- In-Reply-To: {201704191159.v3JBxB2v016688-at-microscopy.com} 8, 50 -- References: {201704191159.v3JBxB2v016688-at-microscopy.com} 8, 50 -- Organization: =?UTF-8?B?TWlrcm9iaW9sb2dpY2vDvSDDunN0YXY=?= AV 8, 50 -- =?UTF-8?B?xIxS?= 8, 50 -- X-Mailer: Claws Mail 3.14.1 (GTK+ 2.24.25; i586-pc-linux-gnu) 8, 50 -- MIME-Version: 1.0 8, 50 -- Content-Type: text/plain; charset=UTF-8 8, 50 -- X-IoP-CAS-MailScanner-Information: Please contact the ISP for more information 8, 50 -- X-IoP-CAS-MailScanner-ID: 57C90D015B6.A97F8 8, 50 -- X-IoP-CAS-MailScanner: Processed 8, 50 -- X-Spam-Status: No 8, 50 -- X-Barracuda-Connect: mail2.biomed.cas.cz[147.231.40.32] 8, 50 -- X-Barracuda-Start-Time: 1492614144 8, 50 -- X-Barracuda-URL: https://barracuda.biomed.cas.cz:443/cgi-mod/mark.cgi 8, 50 -- X-Barracuda-Scan-Msg-Size: 7539 8, 50 -- X-Virus-Scanned: by bsmtpd at biomed.cas.cz 8, 50 -- X-Barracuda-BRTS-Status: 1 8, 50 -- X-Barracuda-Spam-Score: 1.10 8, 50 -- X-Barracuda-Spam-Status: No, SCORE=1.10 using per-user scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=3.0 tests=BSF_RULE7568M, BSF_RULE_7582B, FB_LETTERS_21B 8, 50 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.38191 8, 50 -- Rule breakdown below 8, 50 -- pts rule name description 8, 50 -- ---- ---------------------- -------------------------------------------------- 8, 50 -- 0.10 FB_LETTERS_21B BODY: Special people leave special signs! 8, 50 -- 0.50 BSF_RULE_7582B Custom Rule 7582B 8, 50 -- 0.50 BSF_RULE7568M Custom Rule 7568M 8, 50 -- Content-Transfer-Encoding: 8bit 8, 50 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v3JEqAHD007526 ==============================End of - Headers==============================
I’m trying to round out my understanding of different embedding media and need the latest opinions on Spurr’s LVEM.
I have used it in the past, it was OK, and never worried about it.
Now I see that some workers avoid it due to more toxic ingredients, more difficulty staining, and problems with brittle blocks. Also, like Epon 812 before, I see that some of the original components have been replaced with newer substitutes.
I teach students how to embed tissue and want to be able to tell them the latest opinions from other experts in case I am missing something.
Thanks
Jon
Jonathan Krupp ASB&T San Joaquin Delta College 5151Pacific Ave. Stockton, CA 95207 (209) 954-5284 jkrupp-at-deltacollege.edu
==============================Original Headers============================== 10, 40 -- From prvs=1275be5884=jkrupp-at-deltacollege.edu Wed Apr 19 15:29:05 2017 10, 40 -- Received: from mailin2.deltacollege.edu (mailin2.deltacollege.edu [207.62.175.116]) 10, 40 -- by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v3JKT46R005744 10, 40 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Apr 2017 15:29:05 -0500 10, 40 -- Received: from mailin2.deltacollege.edu (localhost.localdomain [127.0.0.1]) 10, 40 -- by localhost (Email Security Appliance) with SMTP id 89BE8175650_8F7CAF9B 10, 40 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Apr 2017 20:39:21 +0000 (GMT) 10, 40 -- Received: from zmail.deltacollege.edu (zmail.deltacampus.net [10.200.68.180]) 10, 40 -- (using TLSv1.2 with cipher ECDHE-RSA-AES256-GCM-SHA384 (256/256 bits)) 10, 40 -- (Client did not present a certificate) 10, 40 -- by mailin2.deltacollege.edu (Sophos Email Appliance) with ESMTPS id 6C90D173A9C_8F7CAF9F 10, 40 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Apr 2017 20:39:21 +0000 (GMT) 10, 40 -- Received: from localhost (localhost [127.0.0.1]) 10, 40 -- by zmail.deltacollege.edu (Postfix) with ESMTP id CF2666D1E741 10, 40 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Apr 2017 13:30:39 -0700 (PDT) 10, 40 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 10, 40 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10032) 10, 40 -- with ESMTP id XOMOuOHh5UxU for {Microscopy-at-microscopy.com} ; 10, 40 -- Wed, 19 Apr 2017 13:30:39 -0700 (PDT) 10, 40 -- Received: from localhost (localhost [127.0.0.1]) 10, 40 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 62C1C6D1E743 10, 40 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Apr 2017 13:30:39 -0700 (PDT) 10, 40 -- X-Virus-Scanned: amavisd-new at zmail.deltacollege.edu 10, 40 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 10, 40 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10026) 10, 40 -- with ESMTP id 10_cxiZXKJ3k for {Microscopy-at-microscopy.com} ; 10, 40 -- Wed, 19 Apr 2017 13:30:39 -0700 (PDT) 10, 40 -- Received: from [10.246.128.227] (unknown [10.246.128.227]) 10, 40 -- by zmail.deltacollege.edu (Postfix) with ESMTPSA id 41F186D1E741 10, 40 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Apr 2017 13:30:39 -0700 (PDT) 10, 40 -- From: Jonathan Krupp {jkrupp-at-deltacollege.edu} 10, 40 -- Content-Type: text/plain; charset=utf-8 10, 40 -- Subject: Update on Spurr's Plastic Please 10, 40 -- Message-Id: {0F894793-1B18-4B1B-9E76-75ED78A9F395-at-deltacollege.edu} 10, 40 -- Date: Wed, 19 Apr 2017 13:39:20 -0700 10, 40 -- To: Microscopy-at-microscopy.com 10, 40 -- Mime-Version: 1.0 (Mac OS X Mail 9.3 \(3124\)) 10, 40 -- X-Mailer: Apple Mail (2.3124) 10, 40 -- Content-Transfer-Encoding: 8bit 10, 40 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id v3JKT46R005744 ==============================End of - Headers==============================
Check out the Oxford text: high Resolution Electron Microscopy by Spence.
A close second and less Graduate level math is found in "Transmission Electron Microscopy and Diffractory Materials" Springer by Fultz and Howe.
You are looking for a discussion of "Pendellosung". Page 631 Fultz and Howe. To explain it to Students at U of O that I taught, I built a coupled pendulum, where the one pendulum represents the non diffracted beam and the other s Bragg reflected beam. The coupling represents the lattice. The pendulum will stop and start and that time can represent thickness of da sample. And so white spots in HRTEM , nodes, could be channels between atoms or atoms depending on how thick the sample is ( how much time pendulum swings )
Pete Eschbach Oregon State University
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} On Apr 18, 2017, at 4:47 PM, kleopullin-at-email.arizona.edu wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I'm struggling with Williams and Carter (2nd edition, I think) } understanding HRTEM. I generally find their text approachable and easy } to read, but not the HRTEM material, other than the math. } } Is their a text or article that is more detailed? Outside of the } Supposedly HRTEM sources that I have found have lengthy introductions } to the basic, non-HR microscope, then brief descriptions of the math } of HRTEM. } } What's a good Read? } } I'm a microscopist, with a B.S., so technical is okay, but I want a } deep focus on HRTEM, theory and instrumentation. } } Thanks! } } Kleo (Kathleen) Pullin } Moraga, CA } 209-610-0555 } kleopullin-at-email.arizona.edu } https://www.linkedin.com/in/kleopullin } https://twitter.com/resolvingdust } } ==============================Original Headers============================== } 6, 38 -- From kleopullin-at-email.arizona.edu Tue Apr 18 18:31:43 2017 } 6, 38 -- Received: from mail-lf0-f45.google.com (mail-lf0-f45.google.com [209.85.215.45]) } 6, 38 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id v3INVhXS006989 } 6, 38 -- for {Microscopy-at-microscopy.com} ; Tue, 18 Apr 2017 18:31:43 -0500 } 6, 38 -- Received: by mail-lf0-f45.google.com with SMTP id c80so3945116lfh.3 } 6, 38 -- for {Microscopy-at-microscopy.com} ; Tue, 18 Apr 2017 16:41:57 -0700 (PDT) } 6, 38 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 6, 38 -- d=email-arizona-edu.20150623.gappssmtp.com; s=20150623; } 6, 38 -- h=mime-version:from:date:message-id:subject:to; } 6, 38 -- bh=YfdPdRMwFgmXu5BxNvOKsAkoyo+FwZmkn/NoYBoF2y4=; } 6, 38 -- b=BoDK4h27IShxyrd1enChdx9i3aRM10mCT1+5WFi/eEOnTpWZyIGh0pE1g84f1prfv6 } 6, 38 -- dd7Rgexw/nxcbCGZHzVeM9m7zS3TG08A25Zcyrr4SG38tzHvMJn2IcEN/yK2A9KGMo4P } 6, 38 -- ppt4fo5K4EujC2IjSYzDaAwgDo993PSlFfmK354jP8+6biDy5P5Re769GHGlXNxaRFoO } 6, 38 -- vIffa07ko2UYxW2ZtSk32ndCYjGInF7YWeJj0fMr/Q2QH9d8xqGKYlecyBZ9icvq8Gf3 } 6, 38 -- COcKwvK2ekJjd5OclkFdZV12L1iS2TvnPKVY1jdmaSw2KcIeP4hRHaapfyPeZbudzl/9 } 6, 38 -- zQ8A== } 6, 38 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 6, 38 -- d=1e100.net; s=20161025; } 6, 38 -- h=x-gm-message-state:mime-version:from:date:message-id:subject:to; } 6, 38 -- bh=YfdPdRMwFgmXu5BxNvOKsAkoyo+FwZmkn/NoYBoF2y4=; } 6, 38 -- b=gVQL5HThM8f9zp8rMP2VBkjlLysPC5OKKkRq6+r111mxDyJYKFz6j/50hS0G5iuJS8 } 6, 38 -- MESmhhiLeJ+oJWp+J4KzPLvKtJVKZF+UCBPtso7MarAMj0n38n7GA47PVWdntUqiBEnq } 6, 38 -- 4mxXvPxZLYeVkVQ5ovp2l06e7yv1YAkWKT83CZCGmnuOblirQ2NMAoueFWZlB0cPOaxO } 6, 38 -- axGH1jvd2Bvk9Vf/wNhjkEB2Emtm6bH/9THQAheemIXeUpN1xwUi3jDDwcPdZIZqcNDK } 6, 38 -- +by3rgzzHYHLcMa6iaf5kdbcaaTwJcN2NEGXxh9qxW8ajqyyaWNdErCRGL2fn6I8RlRh } 6, 38 -- gM7w== } 6, 38 -- X-Gm-Message-State: AN3rC/4yqfN7txLl177I6Cvh7OECVqoJ5gau5WT9Q4TZXj97g1Ql8ycD } 6, 38 -- qTWclIj/HA1/CdwHeA1RMfP9aM0Ktf8M } 6, 38 -- X-Received: by 10.46.6.17 with SMTP id 17mr2449244ljg.125.1492558916687; Tue, } 6, 38 -- 18 Apr 2017 16:41:56 -0700 (PDT) } 6, 38 -- MIME-Version: 1.0 } 6, 38 -- Received: by 10.25.166.145 with HTTP; Tue, 18 Apr 2017 16:41:56 -0700 (PDT) } 6, 38 -- From: Kathleen Pullin {kleopullin-at-email.arizona.edu} } 6, 38 -- Date: Tue, 18 Apr 2017 16:41:56 -0700 } 6, 38 -- Message-ID: {CAKUce=RSMa2JP0Q3M_9d8zTaBvp_GOx3zQEYmN9FVnRtxLuVXA-at-mail.gmail.com} } 6, 38 -- Subject: HRTEM } 6, 38 -- To: Microscopy-at-microscopy.com } 6, 38 -- Content-Type: text/plain; charset=UTF-8 } ==============================End of - Headers==============================
After trying Epon, I’ve never felt the need to use Spurr's again. For some reason the Spurr’s recipe proportions vary between some vendors. I’ve been really happy with the LX-112 (Epon) from LaddResearch, their mixing instruction are right on point, and I can store the 2 components in syringes in the fridge for about 6 months and just mix them with the accelerator prior to use. Epon seem to section better than Spurr's, and is more stable under the beam. I've also heard good things about the Epon-Araldite mixture.
-Joe
Joe Mowery | Biologist Electron and Confocal Microscopy Unit USDA, Agricultural Research Service Beltsville, MD
-----Original Message----- X-from: jkrupp-at-deltacollege.edu [mailto:jkrupp-at-deltacollege.edu] Sent: Wednesday, April 19, 2017 4:49 PM To: Mowery, Joseph
Greetings
I’m trying to round out my understanding of different embedding media and need the latest opinions on Spurr’s LVEM.
I have used it in the past, it was OK, and never worried about it.
Now I see that some workers avoid it due to more toxic ingredients, more difficulty staining, and problems with brittle blocks. Also, like Epon 812 before, I see that some of the original components have been replaced with newer substitutes.
I teach students how to embed tissue and want to be able to tell them the latest opinions from other experts in case I am missing something.
Thanks
Jon
Jonathan Krupp ASB&T San Joaquin Delta College 5151Pacific Ave. Stockton, CA 95207 (209) 954-5284 jkrupp-at-deltacollege.edu
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Dear All, We are interested in anyone having an old Gatan GIF 200, or a more recent EEL spectrometer for a Tecnai 30, who needs to have it taken off their hands. Thanks,
Ken
Johns Hopkins University
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