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From: microscopy.listserver-at-gmail.com
Date: Sun, 1 Jan 2017 16:27:07 -0600
Subject: [Microscopy] Administrivia: Happy New Year 2017

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Happy New Year Colleagues;

Welcome to the another year of operation of the Microscopy ListServer
a free service to the world wide microscopy community, sponsored
jointly by your Friendly Neighborhood SysOp and the Microscopy Society
of America.

During 2016, the ListServer delivered your messages to more than 4200 subscribers
around the world, with minimal hassels (that I know about). For those of you that are
statistics junkies this year you generated ~ 171+ Gbits of Email traffic and ~ 2.1 Million
Email messages were sent out this year by my tired and old little server. Down abit
from previous years, but still a steady flow.

This year I will be migrating the Listserver to a new server and ISP configuration so
you might see a short outages during testing and transistions.

As usual you don't want to know how much Junk Mail and spam has been filtered out
far more than you might expect. Apologies to those that have problems with
my filters.


The complete Microscopy ListServer Archives for 2016-1994 (~ are on-line at

http://www.microscopy.com.

A couple of IMPORTANT reminders:

If you leave on vacation/holiday use the on-line form to UNSUBSCRIBE
your Email address from the listserver. The out-of-office / on-vacation
autoreply messages are a real nuisance to posters.

Do not reply to message with the return address of:

MicroscopyListserver-noreply-at-microscopy.com

these are messages forwarded usually from the WWW posting form. They do not
go back to the poster but rather into a black hole, which I rarely check.
If you see a message that has this "No-Reply" return address please post your
reply/comment/answer to:

Microscopy-at-microscopy.com

or if you wish to reply privately, look at the username in the body of
the message the originators Email address is usually listed therein.

As always if you have questions about suitability of postings or
are having problems, feel free to contact me at (zaluzec-at-microscopy.com)

Cheers,

Nestor
Your Friendly Neighborhood SysOp


--
===========================================
Do not reply to this message it is from
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============================================

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22, 49 -- Subject: Administrivia: Happy New Year 2017
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From: zaluzec-at-aaem.amc.anl.gov
Date: Wed, 4 Jan 2017 08:22:42 -0600
Subject: [Microscopy] Testing MListserver Forwarding to select Test File 8:26 AM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

8:26 AM

Does this go to archives now that I have fixed the forwarding system???

===========================================
Dr. Nestor J. Zaluzec
Argonne National Laboratory
Photon Sciences Division
9700 S. Cass Ave
Bldg 212 / A-143
Argonne, Illinois 60439 USA
Email: Zaluzec-at-aaem.amc.anl.gov


Tel: 530-NES-TORZ (530-637-8679) has Voice Mail
Lab: 630-252-7901
Fax: 630-252-4798

iChat: Zaluzec-at-AIM.com
Skype: Zaluzec
Polycom: 146.139.72.119
TPM: http://tpm.amc.anl.gov


Senior Scientist - Argonne National Laboratory
Fellow of the Microscopy Society of America
Senior Fellow the Computational Institute - University of Chicago
E.P. Wigner Fellow - Oak Ridge National Laboratory
Past President Microscopy Society of America
Adjunct Professor of Physics - Northern Illinois University &
the University of Illinois at Chicago
Visiting Professor of Microscopy - Manchester University

===========================================
TPMLab: http://tpm.amc.anl.gov
MMSite: http://www.amc.anl.gov
===========================================

The box said ...
"This program requires Win 95/98/NT or better..."
So I bought a Mac !

===========================================
LLAP
=========================================+=====


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From: microscopy.listserver-at-gmail.com
Date: Wed, 4 Jan 2017 08:41:39 -0600
Subject: [Microscopy] Administrivia: Microscopy Listserver Back on-line

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Good Morning Colleagues...

Well my worst nightmares were realized on Monday Night (Jan 2)
when I upgraded my server. Lots of my finely tuned filters
and spam killers "broke". As such the Listserver has been
down for ~ 2 days.

I believe all is fixed now. If you find/identify a problem
please let me know.

Cheers,

Nestor
Your Friendly Neighborhood SysOp



--
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Do not reply to this message it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

============================================

==============================Original Headers==============================
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From: microscopy.listserver-at-gmail.com
Date: Wed, 4 Jan 2017 08:44:33 -0600
Subject: [Microscopy] JB-4 microtime manual

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


Hi all,

Happy New Year!

Does anyone have a manual for a Sorvall Porter-Blum microtome, Type JB-4? If yes, can you please
send me a copy?

best regards,

Beth Richardson

Georgia Electron Microscopy Lab

University of Georgia

Athens, GA


--
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Do not reply to this message it is from
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============================================

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From: microscopy.listserver-at-gmail.com
Date: Wed, 4 Jan 2017 17:48:39 -0600
Subject: [Microscopy] viaWWW:EM facility director position at SDSU

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Email: sbernstein-at-mail.sdsu.edu Name: Sanford Bernstein

Organization: San Diego State University

Title-Subject: EM facility director position at SDSU

Message: Job Announcement: Electron Microscope Facility Lead (Instructional Support Technician III),
College of Sciences - Department of Biology

Job ID:6232; San Diego State University Main Campus; Full-Time Regular

Please note: A completed application is required in order to be considered for this position.

About SDSU: San Diego State University is the oldest and largest higher education institution in the
San Diego region. Since its founding in 1897, SDSU has grown to offer bachelor's degrees in 91
areas, master's degrees in 78 areas and doctorates in 22 areas. SDSU's approximately 35,000
students participate in an academic curriculum distinguished by direct contact with faculty and an
increasing international emphasis that prepares them for a global future.

SDSU is a large, diverse, urban university and Hispanic-Serving Institution with a commitment to
diversity, equity and inclusive excellence. Our campus community is diverse in many ways, including
in terms of race, religion, color, sex, age, disability, marital status, sexual orientation, gender
identity and expression, national origin, pregnancy, medical condition, and covered veteran status.
We strive to build and sustain a welcoming environment for all. SDSU is an equal opportunity employer.
SDSU is seeking applicants with demonstrated experience and/or commitment to teaching and working
effectively with individuals from diverse backgrounds and members of underrepresented groups.

Position Information

Full-time, permanent (probationary) position.
The incumbent is responsible for the day-to-day management of the SDSU Electron Microscope Facility,
oversight of Biology common-use/shared research microscopes (other than EMs), collaborate with and
train users of Biology research microscopes, keep EM Facility and Biology research microscope
equipment funded and operational, order supplies, maintain EM Facility website. The incumbent also
provides input and guidance on the microscopic needs of Biology lab courses.

Salary Range: Anticipated Hiring Salary Range: $4,107 - $6,473 per month (CSU Classification Salary
Range: $4,107 - $6,667 per month). The competitive salary is determined by the education,
experience, and qualifications the candidate brings to the position, internal equity, and the hiring
department's fiscal resources.

Responsibilities:
Training Biology and other SDSU faculty, staff and students in the theory and operation of EM
Facility equipment and other Biology research microscopes
Consulting with Biology and other SDSU faculty and students on research projects using light
microscopy, transmission electron microscopy, and scanning electron microscopy
Oversee external users of the facility and manage the fee-for-service accounting
Collaborating and sharing expertise with Biology faculty and staff to incorporate EM Facility
equipment and other Departmental microscope resources into the Biology curriculum
Maintain equipment associated with EM Facility and Biology research microscopes, coordinate with
repair providers; routine servicing of light microscopes used in biology courses
Acquiring additional instrumentation to enlarge or improve the capabilities of the EM Facility and
Biology research microscopes. This includes submitting grants, or assisting others in the
preparation of grants, to obtain funds for new or updated instrumentation.
Expanding the menu of microscopic techniques the Facility offers, through training and self-study
Maintaining appropriate purchasing procedures, and tracking and billing users of facility
Establishing outreach efforts to area schools and to the community, including designing and
publishing the Facility Web site
Representing the EM Facility and SDSU through service within professional organizations

Knowledge, Skills & Abilities:
Knowledge of the principles and methods related to performing support services.
Knowledge of the principles, information, methods and techniques related to discipline to which
assigned.
Knowledge of the materials and supplies related to the curriculum, their characteristics, and uses.
Ability to plan, organize and schedule work; ability to operate and repair technical and scientific
equipment.
Ability to coordinate support service to meet a comprehensive variety of needs.
Ability to develop off-campus resources related to the discipline for obtaining materials or equipment.
Experience and Education

Equivalent to four years of experience providing instructional support services for a related unit
or discipline, or in producing materials or supplies or repairing equipment in a discipline related
to specialty area to which assigned.

OR

Equivalent to two years of college with 16 semester units in courses involving extensive use of
materials, supplies, or equipment and in a discipline related to the area to which assigned may be
substituted for one year of the required experience.

OR

Equivalent to four years of college with 16 semester units in courses involving extensive use of
materials, supplies, or equipment and in a discipline related to the specialty area to which
assigned may be substituted for two years of the required experience.

Specialized Requirements:
Knowledge of the principles and methods related to light and electron microscopy
Knowledge of the principles, information, methods and techniques related to biological sciences.
Ability to coordinate support service to meet a comprehensive variety of needs of users.
Ability to develop off-campus resources related to the operation, maintenance, and upgrading of
Electron Microscopy Facility equipment Preferred Qualifications

Training/experience in operational design and theory of confocal/epifluorescent microscopy, light
microscopy, scanning and transmission electron microscopy
Experience in preparing samples for confocal/light microscopy, scanning and transmission electron
microscopy
Training/experience operating confocal/light microscopy, scanning and transmission electron microscopy
Experience collecting and processing digital images
Experience purchasing scientific equipment
Relevant Master’s degree
Ph.D. in biological science
Proven abilities in TEM and SEM operations as well as sample preparation techniques for these
microscopes
In addition to knowledge of the principles, information, methods and techniques related to
biological sciences, it is preferable that individual also has knowledge in electron microscopy use
in other scientific and engineering fields.

Application Procedures: Review of applications will begin on Wednesday, January 31, 2017; position
will remain open until filled. The on-line application should be completed in detail. COMPLETION
OF THE ONLINE APPLICATION IS REQUIRED FOR CONSIDERATION, A RESUME ALONE WILL NOT SUFFICE.
Website for applying through the PeopleSoft system:
https://cmsweb.cms.sdsu.edu/psp/HSDPRDF/EMPLOYEE/HRMS/c/HRS_HRAM.HRS_CE.GBL?Page=HRS_CE_HM_PRE&Action=A&SiteId=1

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From: wsalmon-at-wi.mit.edu
Date: Thu, 5 Jan 2017 07:08:12 -0600
Subject: [Microscopy] 2nd Announcement: Analytical and Quantitative Light Microscopy 2017

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues,

As you start this new year, a reminder that this year's run of Analytical and Quantitative Light Microscopy, held at the Marine Biological Laboratory, Woods Hole, MA, USA, will run May 3 - May 12, 2017. Applications are due January 27, 2017.

The application is accessible via the course web site is at http://www.mbl.edu/aqlm. Financial assistance is available.

AQLM is a comprehensive and intensive course in light microscopy for researchers in biology, medicine, and material sciences. This course provides a systematic and in-depth examination of the theory of image formation and application of digital methods for exploring subtle interactions between light and the specimen. This course emphasizes the quantitative issues that are critical to the proper interpretation of images obtained with modern wide-field, confocal microscopes and new emerging technologies.

Laboratory exercises, demonstrations, and discussions include:
• geometrical and physical optics of microscope image formation including Abbe's theory of the microscope and Fourier optics;
• phase contrast, polarization and interference microscopy;
• fluorescence microscopy, quantification of fluorescence, and fluorescent proteins;
• principles and application of digital imaging and quantitative digital image deconvolution;
• digital image processing and object identification and tracking;
• live cell and ratiometric imaging for FRET and ion concentration imaging;
• confocal microscopy and specialized methods such as TIRF and FLIM; and
• new advances in light microscopy such as FCS, PALM, STORM, SIM and Light Sheet.

Within the course, we often refer to AQLM as "Microscopy Boot Camp". We cover every topic with a lecture and an in-depth hands-on lab. It's intense, it's hardcore, and it's really fun.

Why not join us for AQLM (or recommend one of your lab members or core facility users)? You'll never forget it.

Directors: Justin Taraska (National Institutes of Health), Jagesh Shah (Harvard Medical School)
Course Laboratory Director: Wendy Salmon (Whitehead Institute/MIT)

Again, applications due January 27, 2017 and can be access through course web site at http://www.mbl.edu/aqlm.

Cheers,
Jagesh, Justin and Wendy

Visit our Facebook page: http://www.facebook.com/aqlmcourse
Follow us on Twitter: -at-BeadsinMay



Wendy

~~~~~~~~~~~~~~~~~~~~~~~
Wendy Salmon
Light Microscopy Specialist
Whitehead Institute for Biomedical Research
W.M. Keck Imaging Facility
**STREET ADDRESS CHANGE**
455 Main St, Rm 447
Cambridge, MA 02142
c: 617-429-0158
e: wsalmon-at-wi.mit.edu
w: http://staffa.wi.mit.edu/microscopy/


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From: microscopy.listserver-at-gmail.com
Date: Fri, 6 Jan 2017 07:53:26 -0600
Subject: [Microscopy] viaWWW: EDS report shows Boron, even its Boron peak is not present in

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Email: ravi.thakkar369-at-gmail.com Name: Ravi

Title-Subject: [Filtered] EDS report shows Boron, even its Boron peak is
not present in spectrum.

Message: Hi Listeners,
I am using Oxford EDS (SiLi) on Hitachi S 3500N SEM. Surprisingly, I am
getting false Boron atomic% count.(some times values are in negative) in
spectrum report without any detectable peak in the spectrum. Here I am
using Inca 5.05 I am not able to remove the Boron from the element
count table by confirm elements options because absence of boron peak.


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From: microscopy.listserver-at-gmail.com
Date: Fri, 6 Jan 2017 07:54:24 -0600
Subject: [Microscopy] viaWWW:BSD for Zeiss Supra 40

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Email: gary-at-microtechnics.com Name: Dr Gary Gaugler

Organization: Microtechnics

Title-Subject: [Filtered] BSD for Zeiss Supra 40

Message: Hi All:

Does anyone know of BSD options (scintillator or YAG) for the Zeiss
Supra 40? Robinson has retired. A standard Deben Centaurus does not fit
a chamber port without a large extra cost.

Any ideas or suggestions are appreciated.

gary g.


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From: microscopy.listserver-at-gmail.com
Date: Fri, 6 Jan 2017 22:46:04 -0600
Subject: [Microscopy] viaWWW:EDS report shows Boron, even its Boron peak is not present.

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Email: ravi.thakkar369-at-gmail.com Name: Rav

Title-Subject: [Microscopy] viaWWW: EDS report shows Boron, even its
Boron peak is not present.

Message: I am having boron counts even its absent in confirm elements.
Here, I am using Oxford EDS on Hitachi SEM using Inca 5.05
Hope that image file linked to following link will helpful to you to
understand the issue.

https://www.flickr.com/photos/97321550-at-N08/31998854482/in/dateposted-public/

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From: Unique.VisitsCheap-at-mg-style.cn
Date: 09 Jan 2017 17:49:21 -0800
Subject: re: I need Google Analytics Traffic

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New Year's greetings,

At AgResearch, NZ's Government owned agricultural research institute, we have two microscopy related positions with closing dates in the next few weeks (27th Jan for scientist position, and 20th Jan for PhD scholarship).

These should be of interest to people interested the structure/biology of hair and wool (hard-keratin materials).

The scientist position is aimed at an early career scientist who has completed a PhD and has good team skill with a track record in innovative science.

The PhD scholarship focuses on location of hair-specific proteins within the developing fibre using high-pressure freezing and immunolabelling.

Full details of the two positions can be found on AgResearch's website (www.agresearch.co.nz/careers) and there are also links from AgResearch's Linked In jobs page.

Duane

____________________________
Dr Duane P Harland
Senior Scientist
T +64 3 321 8710
E duane.harland-at-agresearch.co.nz
AgResearch Limited
Lincoln Research Centre
Cnr Springs Rd & Gerald Street, Lincoln
Private Bag 4749 Christchurch 8140, New Zealand
T +64 3 325 9900 F +64 3 325 9946 www.agresearch.co.nz
=======================================================================
Attention: The information contained in this message and/or attachments from AgResearch Limited is intended only for the persons or entities to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipients is prohibited by AgResearch Limited. If you have received this message in error, please notify the sender immediately.
=======================================================================


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From Unique.VisitsCheap-at-mg-style.cn Mon Jan 9 19:44:54 2017
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From: microscopy.listserver-at-gmail.com
Date: Wed, 11 Jan 2017 06:46:32 -0600
Subject: [Microscopy] viaWWW:LR White Ultrathin Sections Prepared for ImmunoGold

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Email: m.aindow-at-uconn.edu Name: Mark Aindow

Organization: University of Connecticut

Title-Subject: [Filtered] Postdoctoral Positions in the UConn-FEI Center for Advanced Microscopy and
Materials Analysis

Message: The University of Connecticut (UConn) has partnered with FEI to establish the Center for
Advanced Microscopy and Materials Analysis (CAMMA), which includes a suite of seven new electron-
and ion-beam instruments. CAMMA will form part of the state-of-the-art Advanced Characterization
Laboratories in the UConn Technology Park, which is currently under construction and will open later
this year. Five of the new instruments are currently housed in the Institute of Materials Science at
UConn and will be relocated to the new Technology Park facility later in 2017.
There are two CAMMA postdoctoral positions available immediately:
1. 3D studies of complex microstructures using Xe plasma FIB. This position will involve the
development and exploitation of serial sectioning and data reconstruction strategies using the FEI
Helios plasma FIB. Candidates should have a good working knowledge of FIB and electron microscopy
techniques, including EDXS and EBSD. A clear understanding of microstructural issues in metallic
alloys, ceramics and coatings is important for this post.
2. Microstructural development in aerospace alloys. This position will involve working with local
aerospace industries on a range of projects relating to microstructural issues in Ni, Ti and Al
alloys, and in thermal barrier coatings. Candidates should have extensive experience with relevant
materials systems and with a broad range of electron microscopy techniques. Due to the nature of
some of the projects, only US citizens or permanent residents will be considered for this second
position.

Both of these positions are for one year in the first instance, with the possibility of renewal. To
apply, please send a resume, together with a full list of publications, a statement of relevant
experience, and contact details for three references to Prof. Mark Aindow (m.aindow-at-uconn.edu).
The University of Connecticut’s commitment to excellence is complemented by our commitment to
building a culturally diverse community. We encourage applications from under-represented groups,
including minorities, women, and people with disabilities. The University of Connecticut is an equal
opportunity employer and program provider. Screening of applications will begin immediately and will
continue until the positions are filled.


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From steven.345345.f-at-gmail.com Tue Jan 10 11:19:33 2017
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Email: vakimler-at-oakland.edu Name: Vickie Kimler

Organization: Oakland University Eye Research Institute

Title-Subject: [Filtered] LR White Ultrathin Sections Prepared for ImmunoGold

Message: How long (in terms of days) in advance can I cut ultrathin sections (usually 70-90 nm) in
preparation for immunogold applications? The resin was medium hardness with catalyst, and I
polymerized it in a 55 degree oven overnight.

I was wondering if the surface of the ultrathin section would be altered over a few days after
cutting and antibodies would be somewhat hindered from access to epitopes.

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From: microscopy.listserver-at-gmail.com
Date: Wed, 11 Jan 2017 18:05:05 -0600
Subject: [Microscopy] viaWWW:Bio TEM Workshop University of Georgia

Contents Retrieved from Microscopy Listserver Archives
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Name:
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School:
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Grade/Education Level:
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Email:
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Email: jpshield-at-uga.edu Name: John Shields

Organization: University of Georgia

Title-Subject: [Filtered] Bio TEM Workshop -

Message: Biological TEM Workshop

This intensive, three-day workshop will provide a practical and basic theoretical introduction to
the Transmission Electron Microscope and biological sample preparation techniques. Each day will
consist of lecture, discussion and hands-on training led by GEM staff.
What: Anyone requiring training on TEM and biological sample preparation. The workshop will be
limited to 6 participants based on the availability of equipment.
When: Monday through Wednesday, March 8-10, 2017, 8am-5pm each day (lunch is provided)

Where: 115 D.E. Brooks Drive, 154 Barrow Hall, University of Georgia, Athens, GA 30602

Registration: Contact John Shields (jpshield-at-uga.edu) for more information and to sign up.
Registration requires iLab account through the GEM website. https://uga.ilabsolutions.com/account/login

Deadline: February 27, 2017


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From: microscopy.listserver-at-gmail.com
Date: Wed, 11 Jan 2017 18:07:06 -0600
Subject: [Microscopy] viaWWW:Invitation to submit abstracts to the Celtic Sessions at

Contents Retrieved from Microscopy Listserver Archives
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X-from: Ursel.Bangert-at-ul.ie


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Email: Ursel.Bangert-at-ul.ie Name: Ursel Bangert

Organization: University of Limerick

Title-Subject: [Filtered] Invitation to submit abstracts to the Celtic Sessions at mmc2017

Message: Microcscience Microscopy Congress 2017 – Celtic Sessions

The Microscopy Society of Ireland (MSI) is co-organising two sessions (together with the Scottish
Microscopy Group, SMG) at the Microscience Microscopy Congress (mmc2017), organised by the Royal
Microscopical Society. The event will take place in Manchester, UK, from July 3rd-6th. The
international mmc2017 conference will comprise over 30 symposia, with excellent speakers and vibrant
supporting poster sessions. Further to presenting new research in Ireland and Scotland, the Celtic
Sessions are aimed at showcasing and encouraging collaborative research, and all members of the
international microscopy community are sincerely invited to contribute to the Celtic Sessions, which
will include invited and regular talks, as well as flash presentations and poster sessions. The
abstract submission for mmc2017 is now open. We are inviting abstracts for the Celtic Sessions:
•Biological Processes at the Nanoscale •Inorganic Nanomaterials
For more information about how to participate and submit abstracts, please go to the MSI website
http://microscopy.ie/ or http://microscopy.ie/2017.php, or submit your abstract directly through
the mmc website http://mmc-series.org.uk/about/news/abstract-submission-now-open or through the
official mmc2017 flyer http://microscopy.ie/Doc/MSI_Call_for_Papers_Flyer2.pdf.

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From: conversion.seo-at-gmail.com
Date: 12 Jan 2017 04:30:22 -0800
Subject: re: Boost Sales with Social Media Marketing

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Hmm...

} Dear sir/madam, For a masters course we have to find the market size for a planar positioning stage. We are having trouble finding sales numbers or estimating how many microscopists use an automatic positioning stage with their microscope or how many would like to use one if the price is right. Hopefully you could provide us with some global estimates. Kind regards, Jacar Brocker (4079450)
}

Global estimates?

Does asking someone else to do the research work for one's degree allow
one to earn it, I wonder?

And a Masters in marketing at that.

Ask A Microscopist may be a reasonable start, though asking around the
University would probably work even better.


Find someone who may use or be interested in product and talk with them.
When you have learned what you and they want to learn, ask if they know
someone else with whom you can talk.
Repeat the above until you have learned enough.


No number of degrees, doctorates or whatever will avoid that. If you
want to succeed at sales, marketing, engineering, science and a number
of other disciplines, you must ignore the knot in your stomach, ignore
the fear of failure, embarrassment or humiliation, get out there and
talk to people. In practice, most of the time there will not be
failure, embarrassment or humiliation. Generally, people will be pleased
you take an interest. For many people, a coffee, croissant and 20
minute break from work makes a nice change, too.

An ex colleague of mine used sometimes to come back from meetings with
potential or actual customers, suppliers, partners or whatever and use
the term "WoFT". If one meets with people in those circumstances it is
for each party to learn, even if what one learns is that nobody wants
the product. IMHO, the only party to whom "WoFT" can apply is oneself.

Gordon.

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From: microscopy.listserver-at-gmail.com
Date: Mon, 16 Jan 2017 17:59:28 -0600
Subject: [Microscopy] viaWWW:LR White Ultrathin Sections Prepared for ImmunoGold

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Organization: Florida State University

Title-Subject: Postdoc position available

Job Title: Post-Doctoral Associate - Electron Microscopist

Summary Description:

Florida State University (FSU) and National High Magnetic Field Laboratory (NHMFL) invite applications for a post-doctoral research associate position to undertake research projects led by Prof. Fumitake Kametani. The research projects aim to understand the nano- and atomic-structural correlations to the material properties by using the advanced electron microscopes including aberration-corrected atomic resolution (scanning) transmission electron microscope (S/TEM) and focused ion beam (FIB). A person who has an experience in the atomic and nano-structural analyses of complicated materials such as high temperature superconductors, carbon nanotube composites, perovskite thin films or energy-related materials is encouraged to apply. This position requires expertise in atomic resolution imaging in a TEM/STEM and nano-fabrication by using a FIB. As listed below, we already have the state-of-art electron microscopes here, so he/she is expected to start working material problems right away.

This position is a full time position with an anticipated starting salary at a minimum of $48,000 and includes health benefits.

Major microscope-related instruments at FSU and NHMFL:
* JEOL ARM200cF Cs-corrected S/TEM with Electron Energy Loss Spectroscopy (EELS) and Energy-dispersive X-ray Spectroscopy (EDS)
* Carl Zeiss 1540EsB field emission scanning electron microscope and FIB equipped with Gas Injection System and OmniProbe in-situ lift-out tool
* Gatan PIPS ion mill
* Micro Support Axis Pro SS ex-situ lift-out tool
* JEOL IB-19500 CP ion cross section polisher

Requirements:
* Ph.D. in materials science, physics, engineering, chemistry, or related field.
* Must have experience to operate TEM.
* Must have experience preparing specimens using FIB.
* Preferred to have experience operating Cs-corrected STEMs.
* Preferred to have experience in EELS.

Please directly send CV including 2 references to:

Fumitake Kametani
Associate Professor
Department of Mechanical Engineering, Florida State University,
and Applied Superconductivity Center, National High Magnetic Field Laboratory
2031 E. Paul Dirac Dr., Tallahassee, FL 32310
Tel: (850) 645-7491
E-mail: fkametani-at-fsu.edu


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Email: vakimler-at-oakland.edu Name: Vickie Kimler

Organization: Eye Research Institute

Title-Subject: [Filtered] LR White Ultrathin Sections Prepared for ImmunoGold

Message: Hello,
Second Request - Not sure if the first message was transmitted...
What is the maximum time I need to wait in order to apply immunogold reagents (buffers, etc.) to
ultrathin sections of LR White blocks (Medium hardness with catalyst?
Thanks much,
Vickie

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From: leunissen-at-aurion.nl
Date: Mon, 16 Jan 2017 18:20:18 -0600
Subject: [Microscopy] Re: viaWWW:LR White Ultrathin Sections Prepared for ImmunoGold

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Good day Vickie,

I do not think there is such a thing as a maximum time you have to wait before which LR white sections would be suited for immuno. Perhaps you are wondering if there is a minimum time before sections are suited? As far as our experience goes LR-white and Lowicryl sections seem to keep pretty much indefinitely. Perhaps it would be wise if they were kept in a dessicator. I do not think there is a minimum time one should consider having to wait. If things change post-sectioning I would expect epitopes to change (oxidize?) and that hardly ever is beneficial. And how would ‘buffers etc’ relate to that, I do not understand?

Are you getting results, or not getting results, that make you wonder whether section age plays a role?

If you like, please feel free to get in touch off-list. I will be happy to help.

Jan Leunissen
- - -
Aurion - ImmunoGold Reagents
www.aurion.nl





==============================Original Headers==============================
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From: tina-at-pbrc.hawaii.edu
Date: Mon, 16 Jan 2017 19:27:16 -0600
Subject: [Microscopy] Re: viaWWW:LR White Ultrathin Sections Prepared for

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi, Vickie-

I'm sure it all depends on the epitope.

Here's a story: Someone once had LR-White sections that were more than two
years old, from another institution. They went ahead and applied their
antibody, secondary antibody and gold, and seemed to have every expectation
that it would work (whether based on experience or blind faith, I do not know).
However, they needed images immediately for a grant proposal, or at least they
needed to know if a particular protein was on a particular organ in the squid.
My TEM (the only one in the state) was down. In a panic, we stuck them in the
SEM wehere we could see the gold particles on the surface of the section, and
we could also see outlines of the ultrastructure because parts of the tissue
had bulged out of the resin enough to give some relief. In fact, it looked
really cool. And they were able to state that the protein was in that organ. If
you've ever sectioned a block and then let it sit for some time (hours,
years?), and than sectioned again you've probably noticed that some tissues
expand out further than others over time.

Anyway, in this case I'm sure their antibody would have worked right away on
freshly-cut sections as well as on sections that had been sitting on grids for
more than two years. The resin may flow somewhat over time. Your mileage
may vary. Perform a bunch of timing experiments and report back!

Aloha,
Tina

} Email: vakimler-at-oakland.edu Name: Vickie Kimler
}
} Organization: Eye Research Institute
}
} Title-Subject: [Filtered] LR White Ultrathin Sections Prepared for ImmunoGold
}
} Message: Hello,
} Second Request - Not sure if the first message was transmitted...
} What is the maximum time I need to wait in order to apply immunogold reagents
} (buffers, etc.) to
} ultrathin sections of LR White blocks (Medium hardness with catalyst?
} Thanks much,
} Vickie
}

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From: les-at-zsgenetics.com
Date: Tue, 17 Jan 2017 13:27:06 -0600
Subject: [Microscopy] curtain systems

Contents Retrieved from Microscopy Listserver Archives
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Hello,
I wanted to know if anyone has had experience using curtain wall systems to
segment a space for microscopes without building hard walls. I want to make
a somewhat-temporary home for an instrument. Laser safety curtaining is
common but way too expensive and unnecessary, since we have no lasers. Has
anyone used any of the industrial-type curtain systems to divide up a space?
Thanks for any information.

Regards,
Larry Scipioni
ZS Genetics


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From: bicarbaj-at-mtholyoke.edu
Date: Tue, 17 Jan 2017 13:53:10 -0600
Subject: [Microscopy] EM: wall-mounted fume extractors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello everyone,

I am considering purchasing a wall-mounted fume extractor for my
microwave tissue processor which I will use for EM and paraffin work
(to save space in my fume hood).

I have found some filters that have been rated for use with PFA and
GA, but I am waiting to hear from the manufacturer regarding their use
for OSO4, acetone, resins, and PO (doubt that info is available). I'd
like to assume that the filters ("special carbon-treated") effectively
filter out all of these, but I need to be as certain as possible. I
will have undergraduates preparing samples and hope to use the
microwave in my TEM and SEM courses, so I am very concerned about
student safety.

Do any of you have any experience using these systems? Are they safe?
Should I stop being so worried and just buy it? :)

Thank you!
-Blanca
-----------------------------------------
Blanca Carbajal Gonzalez, M.S.
Director of Microscopy
Science Center
50 College St
Mount Holyoke College
Clapp Laboratory 123
Office: 413-538-3118
Cell: 559-905-7138
bicarbaj-at-mtholyoke.edu
MHC Microscopy

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From: vray-at-partbeamsystech.com
Date: Tue, 17 Jan 2017 15:06:28 -0600
Subject: [Microscopy] Re: EM: wall-mounted fume extractors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Blanca,

Why don't you run HVAC flex pipe from your wall-mounted fume extractor
and plug it into the exhaust system? Failing that - modify one frame in
the nearest window and exhaust filtered air outside. Decent carpenter or
HVAC tech should be able to make such setup. Be safe :)


Valery Ray
www.linkedin.com/in/valeryray
==============================
PBS&T, MEO Engineering Company
290 Broadway, Suite 298
Methuen, MA 01844, USA
Phone: +1-978-305-0479 - leave a message
Mobie: +1-978-305-0479 - leave a message
E-mail: vray-at-partbeamsystech.com
Web: www.partbeamsystech.com
Web: www.freudlabs.com

On 1/17/2017 2:54 PM, bicarbaj-at-mtholyoke.edu wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Hello everyone,
}
} I am considering purchasing a wall-mounted fume extractor for my
} microwave tissue processor which I will use for EM and paraffin work
} (to save space in my fume hood).
}
} I have found some filters that have been rated for use with PFA and
} GA, but I am waiting to hear from the manufacturer regarding their use
} for OSO4, acetone, resins, and PO (doubt that info is available). I'd
} like to assume that the filters ("special carbon-treated") effectively
} filter out all of these, but I need to be as certain as possible. I
} will have undergraduates preparing samples and hope to use the
} microwave in my TEM and SEM courses, so I am very concerned about
} student safety.
}
} Do any of you have any experience using these systems? Are they safe?
} Should I stop being so worried and just buy it? :)
}
} Thank you!
} -Blanca
} -----------------------------------------
} Blanca Carbajal Gonzalez, M.S.
} Director of Microscopy
} Science Center
} 50 College St
} Mount Holyoke College
} Clapp Laboratory 123
} Office: 413-538-3118
} Cell: 559-905-7138
} bicarbaj-at-mtholyoke.edu
} MHC Microscopy
}
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} 5, 37 -- From: Blanca Carbajal Gonzalez {bicarbaj-at-mtholyoke.edu}
} 5, 37 -- Date: Tue, 17 Jan 2017 14:57:50 -0500
} 5, 37 -- Message-ID: {CAO94sumJEJEG0mw+2yoewmQSQRDodJE5CkOazdHvbhxzZB9vvg-at-mail.gmail.com}
} 5, 37 -- Subject: EM: wall-mounted fume extractors
} 5, 37 -- To: microscopy-at-microscopy.com
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5, 35 -- From vray-at-partbeamsystech.com Tue Jan 17 15:06:27 2017
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5, 35 -- Subject: Re: [Microscopy] EM: wall-mounted fume extractors
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5, 35 -- References: {201701171954.v0HJsT6C009676-at-microscopy.com}
5, 35 -- From: Valery Ray {vray-at-partbeamsystech.com}
5, 35 -- Cc: microscopy-at-microscopy.com
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From: microscopy.listserver-at-gmail.com
Date: Thu, 19 Jan 2017 18:08:32 -0600
Subject: [Microscopy] viaWWW CryoEM :Position available in San Diego, California

Contents Retrieved from Microscopy Listserver Archives
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X-from: mreedman-at-rms.org.uk

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Email: mreedman-at-rms.org.uk Name: Mel Reedman

Organization: Royal Microscopical Society

Title-Subject: [Filtered] MSM XX Abstract Submission Extended - Microscopy of Semi Conducting Materials

Message: Abstract deadline extended to Sunday 22 January and Registration now open!
Microscopy of Semiconducting Materials XX (MSM XX)
9-13 April 2017
Lady Margaret Hall, University of Oxford

www.rms.org.uk/msm-xx

Scientific Organiser: Thomas Walther
The conference will focus on the most recent advances in the study of the structural and electronic
properties of semiconducting materials by the application of transmission and scanning electron
microscopy. The latest developments in the use of other important micro-characterisation techniques
including scanning probe microscopy, X-ray topography and diffraction will also be featured.
Developments in materials science and technology covering the complete range of elemental and
compound semiconductors will be described.
Submit your abstract before midnight on Sunday 22 January 2017.
We are accepting abstracts for both submitted talks and poster presentations at MSM XX on the
following topics:
- Analytical TEM
- CL
- Lattice Defects
- Poly and Nano Crystals
- Thin Films
- Strained Layers and QWs
- Nanowires
- SPM & APFIM
- SEM & FIB
- Advanced Devices

Registration
Registration for this meeting is now open. To register your place, please visit www.rms.org.uk/msm-xx.
The registration fees are as follows:

Standard rate 575
RMS/IoP Member rate 475
Student rate 325
RMS/IoP Student member rate 280

To book en-suite accommodation for 75 a night directly with Lady Margaret Hall, please visit
http://conference.lmh.ox.ac.uk/accommodation/. Use the code MSM2017, to show availability and take
advantage of the preferential room rate.
Find out more, submit an abstract and register at www.rms.org.uk/msm-xx


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18, 53 -- Subject: viaWWW:MSM XX Abstract Submission Extended - Microscopy of Semi
18, 53 -- Conducting Materials
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From ronasyre474-at-gmail.com Wed Jan 18 09:59:01 2017
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Email: erica-at-scripps.edu Name: Erica Ollmann Saphire

Organization: The Scripps Research Institute

Title-Subject: [Filtered] Position available in San Diego, California

Message: Position available for an experienced staff scientist (or postdoc) to perform single
particle cryoEM studies on proteins relevant for viral hemorrhagic fever pathogenesis and medical
defense.

You will have primary responsibility for data collection and oversight of a dedicated 300 keV Titan
Halo with a Falcon 2 direct detector and for training of incoming students to the lab.
In particular, we are looking for someone with strong interest and expertise in instrumentation,
good communication skills, and a collaborative spirit who could pursue their own projects and assist
those of others.

Our lab studies the proteins of viruses like Ebola, Marburg and Lassa, using structural biology and
cellular analysis to understand how the very few proteins encoded by these viruses achieve a
tremendous array of functions through the virus life cycle. Although we are primarily a structural
biology lab ourselves, we direct a five-continent collaboration of academic, industrial and
government scientists and clinicians. Hence, there is the opportunity here to see how the structures
fit into the cellular, organismal and population contexts and to use structural biology as a roadmap
to devlop much-needed vaccines and therapeutics.

Applications should be sent via email to erica-at-scripps.edu and should include a c.v., list of
publications, names of references and a letter of interest. If you have any questions, just email me!

Erica Ollmann Saphire, Ph.D.
Professor, Immunology & Microbial Science
Co-Director, Center of Excellence, The Global Virus Network
Director, Viral Hemorrhagic Fever Immunotherapeutic Consortium

The Scripps Research Institute
10550 North Torrey Pines Road, IMM-21
La Jolla, CA 92037
Tel: (858)784-8602
erica-at-scripps.edu

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17, 52 -- Subject: viaWWW CryoEM :Position available in San Diego, California
17, 52 -- References: {201701192020.v0JKKk56030157-at-microscopy.com}
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From: microscopy.listserver-at-gmail.com
Date: Thu, 19 Jan 2017 18:10:12 -0600
Subject: [Microscopy] viaWWW:TEM Application Scientist Opening

Contents Retrieved from Microscopy Listserver Archives
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Email: jhyun-at-gatan.com Name: John Hyun

Organization: Gatan

Title-Subject: [Filtered] TEM Application Scientist Opening

Message: Location: Pleasanton, CA (remote locations considered)

Position Summary:
This position is responsible for supporting the sales and marketing efforts of Gatan’s highly
successful Analytical TEM product line (GIF and EELS systems). The position is based at Gatan’s
Pleasanton, CA headquarters but will support analytical products worldwide.

Job Responsibilities:
•Provide pre-sales applications support with presentations and demonstrations of Gatan products on a
range of TEM models
•Provide post-sales customer support and on-site customer training
•Provide on-site advanced customer training and telephone/email support
•Provide general applications support, telephone/email support, and limited hardware and software
troubleshooting
•Propose design enhancements and improvements to Gatan hardware and software
•Work with Gatan R&D in the development and testing of new products and applications
•Represent Gatan at scientific conferences

Education/Experience:
•Advanced degree in science or engineering (or equivalent experience) is required
•Strong background in transmission electron microscopy, specifically in relation to the acquisition,
application, interpretation, and discussion of analytical TEM techniques including EELS, EDS, EFTEM,
and STEM techniques
•Hands-on, post-graduate level experience in advanced analytical TEM applications with a proven
publication record Qualifications:
•Ability to interface effectively with customers at all experience levels while projecting a strong
client service attitude
•Excellent verbal, written, and interpersonal communication skills in English are essential
•Ability to work on complex learning and development problems as well as teach highly technical
information is essential
•Significant domestic and international travel required- 50% or higher at times.

To Apply:
Interested candidates should submit a resume to hrus-at-gatan.com.
Gatan is an equal opportunity employer. All qualified applicants will receive consideration for
employment without regard to race, religion, color, national origin, sex, sexual orientation, gender
identity, age, status as a protected veteran, among other things, or status as a qualified
individual with disability.

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From: microscopy.listserver-at-gmail.com
Date: Sat, 21 Jan 2017 14:51:53 -0600
Subject: [Microscopy] viaWWW: Gatan ChromaCL Help Needed

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Email: heckman-at-bgsu.edu
Name: Carol Heckman

Organization: Bowling Green State University

Title-Subject: [Filtered] Gatan ChromaCL

Message: We bought a GATAN cathodoluminescence detector some years ago, and had it installed on our
Hitachi 2700S SEM. It breaks vacuum sometimes when it is entered into the column. We need
cathodoluminescence mode for several projects.

Does anybody have the same problem? If so, what did you do and did you solve it?


Carol Heckman


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From: james.passmore-at-sealedair.com
Date: Mon, 23 Jan 2017 08:10:43 -0600
Subject: [Microscopy] XPS/ESCA system for donation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Carol,

Since the problem is intermittent and occurs during insertion, chances
are you have either crack in vacuum bellows, or warn-out O-ring
somewhere in feedthrough. You would probably need to call in either OEM
or third-party service, or find someone local who can bring in a leak
detector and troubleshoot this on site.

Best Wishes :)

Valery Ray
www.linkedin.com/in/valeryray
==============================
PBS&T, MEO Engineering Company
290 Broadway, Suite 298
Methuen, MA 01844, USA
Phone: +1-978-305-0479 - leave a message
Mobie: +1-978-305-0479 - leave a message
E-mail: vray-at-partbeamsystech.com
Web: www.partbeamsystech.com
Web: www.freudlabs.com

On 1/21/2017 3:53 PM, microscopy.listserver-at-gmail.com wrote:
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}
} Title-Subject: [Filtered] Gatan ChromaCL
}
} Message: We bought a GATAN cathodoluminescence detector some years ago, and had it installed on our
} Hitachi 2700S SEM. It breaks vacuum sometimes when it is entered into the column. We need
} cathodoluminescence mode for several projects.
}
} Does anybody have the same problem? If so, what did you do and did you solve it?
}
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} Carol Heckman
}
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5, 35 -- From vray-at-partbeamsystech.com Sat Jan 21 16:57:54 2017
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5, 35 -- To: heckman-at-bgsu.edu
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5, 35 -- Cc: microscopy-at-microscopy.com
5, 35 -- From: Valery Ray {vray-at-partbeamsystech.com}
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From sawijama5-at-gmail.com Sat Jan 21 19:21:49 2017
Return-Path: {sawijama5-at-gmail.com}
Received: from gmail.com ([210.92.18.86])
by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v0M1LkoN032594
for {microscopylistserverarchive6-at-microscopy.com} ; Sat, 21 Jan 2017 19:21:48 -0600
Message-ID: {18CCB40A.A1DE92E8-at-gmail.com}

All,
For those interested in the microanalysis side of things, I have
available to donate a Physical Electronics Quantum 2000 Scanning ESCA
Microprobe. The Quantum is the predecessor to the PHI Quantera model.

This X-ray Photoelectron Spectroscopy/Electron Spectroscopy for
Chemical Analysis system has been sitting powered down but connected
to clean nitrogen for a little over a year, after developing a problem
in a small power supply. That power supply has been priced at about
$300. Prior to that, it had a noise issue, so I believe there is a
problem with the detector or in the detector electronics that would
need to be addressed. The unit was purchased in 1999 for
approximately $600,000, and used for several years with very clean
samples. In 2006 it was upgraded to a Windows XP data system.

My company is relocating R&D facilities from Duncan, SC (the current
instrument location) and no longer has pressing need for the
technique. Rather than relocate the instrument, we would like to
donate it to a university or other non-profit. Recipient would be
responsible for transportation and related costs. As part of an
effort to give back to our local community, preference would be given
to schools in closer proximity to our new headquarters in Charlotte,
NC, but all interested parties are encouraged to inquire by emailing
me at james.passmore+xps-at-sealedair.com.

Regards,
Jim

--
Jim Passmore
Principal Scientist, R&D

Sealed Air Corporation
P: 864.433.2927
100 Rogers Bridge Rd., Bldg A
Duncan, SC 29334

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From: microscopy.listserver-at-gmail.com
Date: Mon, 23 Jan 2017 18:14:02 -0600
Subject: [Microscopy] viaWWW: Position Open : Research Associate I (28N) - Electron

Contents Retrieved from Microscopy Listserver Archives
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Email: cni-at-udel.edu Name: Chaoying Ni

Organization: University of Delaware

Title-Subject: [Filtered] Research Associate I (28N) - Electron Microscopy

Message: Research Associate I - (Grade 28N) Materials Science and Engineering

Deadline: January 26, 2017

CONTEXT OF THE JOB:

Under limited supervision, performs a variety of complex technical laboratory duties including the
design and construction of test equipment. Supervises and trains personnel. The principal emphasis
is on working independently and exercising personal initiative in conducting experiments using
complex electron and light microscopes, scanning probe microscopes and associated sample preparation
equipment and experiment devices.

QUALIFICATIONS:

Requires a minimum of a Bachelor's degree and one year of experience in laboratory work in a
relevant field as well as experience with the necessary physical, electronic and chemical systems.
Supervisory experience preferred.
Related progressive experience beyond a high school diploma or GED may be substituted for required
education or additional related education may be substituted for required experience. Requires
knowledge of laboratory techniques, procedures and instrumentation.

Requires abilities to read and interpret complex operations manuals, drawings and design, interpret
problems and design equipment for the particular tasks, assume responsibility and take initiative to
perform tasks, work independently, train and instruct others, and communicate effectively and
interact well with people of all ages and diverse backgrounds.

OCCUPATIONAL EXPOSURES:

May be required to use personal protective equipment to prevent exposure to hazardous materials.


More details:
https://udjobs.nss.udel.edu:4450/psc/RESUME/EMPLOYEE/HRMS/s/WEBLIB_UDEL.ISCRIPT1.FieldFormula.IScript_Careers?Page=HRS_CE_HM_PRE&Action=A&SiteId=888&

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From: microscopy.listserver-at-gmail.com
Date: Mon, 23 Jan 2017 18:15:00 -0600
Subject: [Microscopy] viaWWW:Nano Technology Sales Position

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Email: mikeh-at-ncimicro.com Name: Mike Hehr

Organization: NCI

Title-Subject: [Filtered] Nano Technology Sales Position

Message: We are currently seeking an individual with EM Sample Prep experience for a new technical
sales position. This includes, Ion Milling, Ultra Microtomy, CLEM and High Pressure Freezing. This
role will be to support customers in the vast Midwest. If interested, please submit you resume to
mikeh-at-ncimicro.com

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From: jcarroll-at-murraystate.edu
Date: Wed, 25 Jan 2017 14:29:42 -0600
Subject: [Microscopy] TEM negative staining issues

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,

Those of you actively working with FIB in either device or analytical applications could find the forwarded below message of interest:

--- On Tue, 1/24/17, Hugo Bender (imec) {Hugo.Bender-at-imec.be} wrote:

} From: Hugo Bender (imec) {Hugo.Bender-at-imec.be}
} Subject: new link to EFUG website : http://efug.imec.be/
} To: "Hugo Bender (imec)" {Hugo.Bender-at-imec.be}
} Date: Tuesday, January 24, 2017, 1:18 AM
}
} Dear EFUG’ers,
} FIB’ers,
}  
}  
} Please note that the link to the
} EFUG website is changed to : http://efug.imec.be/
}
} The old link will soon not be
} operational anymore.  So update your bookmarks
} now!
}  
}  
} There will be again 2 FIB meetings
} in Europe in 2017:
}  
} 1st  EUFN
} workshop
} (Former DACH
} Workshop) : general FIB and FIB
} applications
} 4-5 July 2017, Graz, Austria.
}
} Abstract : deadline 30 April 2017
} http://www.eu-f-n.org/
}
}
} 21st
} EFUG meeting
} during ESREF        :
} semiconductor and device applications of
} FIB
} Week 25-29 September 2017,
} Bordeaux France
} http://efug.imec.be/
}  
}  
} Please forward to your FIB
} colleagues !
}  
}  
} Best regards
}  
} Hugo
}  
}  
} Hugo
} Bender
} Principal Member
} Technical Staff
} T +32 16 28
} 1304
} hugo.bender-at-imec.be 
} I 
} www.imec.be
} Kapeldreef 75 
}
} I  3001
} Leuven 
} I 
} Belgium
}
} imec e-mail
} disclaimer
}  
}
}  
}
}
}


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From michcons458-at-gmail.com Tue Jan 24 16:35:33 2017
Return-Path: {michcons458-at-gmail.com}
Received: from gmail.com ([210.92.18.86])
by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v0OMZUvo027260
for {microscopylistserverarchive6-at-microscopy.com} ; Tue, 24 Jan 2017 16:35:32 -0600
Message-ID: {4149520D.C242A3C7-at-gmail.com}

I am a relatively inexperienced TEM tech and am having problems with my
PTA. Each time I make fresh PTA, I end up with an image full of stain
blobs. Obviously this is useless. I have tried purchasing fresh PTA, but
that didn't make any difference. I have also used BSA, which helps some,
but not a lot. Help! Any ideas are welcome.

Jami

Jami Carroll
Lab Tech II - Virology
Breathitt Veterinary Center
Hopkinsville, KY
270-886-3959
jcarroll-at-murraystate.edu

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From: FMonson-at-wcupa.edu
Date: Wed, 25 Jan 2017 15:42:07 -0600
Subject: [Microscopy] TEM negative staining issues

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Jami,

I do hope that you have consulted Google with: "PTA negative staining" to try to find some troubleshooting advice from experts. What I am about to offer is all true and necessary for me. Thus, necessary for everyone else - if I don't think about your problem.

I can state with absolutely strictly personal experience, that one - Me! - throws time away all the time if the water one - Me, again - starts with has not been deionized, glass distilled, filtered with the 22um ['micro' or 'Meuw' meter] 57mm Millipore filter, and, if to be used in MolBio, laced with RNAse Inhibitor [https://en.wikipedia.org/wiki/Ribonuclease_inhibitor ]. Once this 'Monkish' procedure has been completed with both song and dance, I can be assured of a good experiment.

If the 'blobs' are larger than .22um, then I recommend that you run your PTA thru a 0.22um 12mm Millipore filter using a small Swinney holder with a 1ml disposable syringe. You could also try a dose of high frequency sonic jewelry cleaner borrowed from your Mom or your 'gal.'

I have given you both mystic and sound recommendations to attack your problem. These are things I have tried in the past, but, I admit, nowhere near Kentucky. [In the old days, histologists who Wintered in Maine and Summered in Florida, had two types of paraffin: one for Maine; and a second for Florida - each with a 'Climatized' paraffin recipe!

I do humarize when the subject permits, so, please understand that I am old (77), sometime called "Grumpy39," and cannot restrain myself any more.

Cheers, and good luck,

Fred Monson

P.S. If you come to a moment where you simply have to change brains, sit down and answer this simple question: "How many digital cameras do you have with you right now? After you answer, please take a few minutes to determine why some old guy would ask such a silly question to take my mind of my problem with PTA. There you go. My answer is that 98% of educated people give the wrong answer. You should know that I only gave ONE multiple answer test in the 12 years that I instructed in biology in development and electron microscopy. In histology, I felt obligated to remind students that all they would learn in histology would be about dead stuff. Cheers, again.

P.S. With that cheerful business done, please remember that life/death of virions is still under misunderstanding. Be careful - always.
P.S. In the creek, doped with E. Coli, for each of them there are 100 phage.
P.S. Oh! Yes! If you have a need of concentrating the sample, consider a Beckman Airfuge with a TEM head.

Frederick C. Monson, PhD
Technical Director
Center for Microanalysis and Imaging, Research and Training (CMIRT)
West Chester University of PA
Geology-Astronomy
750 South Church St.
West Chester, PA, 19383
fmonson-at-wcupa.edu
610-738-0437(Work)
Fc.monson-at-gmail.com for more help if you need it: i.e., refereces, and other stuff.






-----Original Message-----
X-from: jcarroll-at-murraystate.edu [mailto:jcarroll-at-murraystate.edu]
Sent: Wednesday, January 25, 2017 3:41 PM
To: Monson, Frederick {FMonson-at-wcupa.edu}

I am a relatively inexperienced TEM tech and am having problems with my PTA. Each time I make fresh PTA, I end up with an image full of stain blobs. Obviously this is useless. I have tried purchasing fresh PTA, but that didn't make any difference. I have also used BSA, which helps some, but not a lot. Help! Any ideas are welcome.

Jami

Jami Carroll
Lab Tech II - Virology
Breathitt Veterinary Center
Hopkinsville, KY
270-886-3959
jcarroll-at-murraystate.edu

==============================Original Headers==============================
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From: rongchigram79-at-yahoo.com.sg
Date: Thu, 26 Jan 2017 02:46:34 -0600
Subject: [Microscopy] Temperature Logger to Recommend

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Dear All, i would like to ask for your recommendation for a temperature logger. Probably a reasonable sensitivity, acuracy and precision and is able to monitor live using the TEM pc. I am not sure if such system will interfere any performance of the TEM. If you encounter such problem, is it possible to share your experience? I do know some logger has wifi/bluetooth capability, will this interfere the TEM performances well? whiCurrently we have a temperature logger but its not live and we have to extract the data out from time to time which is very troublesome.

Cheers,
Yee Yan, Tay
FACTS Lab
NTU
Singapore

==============================Original Headers==============================
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From: leunissen-at-aurion.nl
Date: Thu, 26 Jan 2017 03:04:45 -0600
Subject: [Microscopy] Re: Temperature Logger to Recommend

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,

you may want to talk to Guenter Resch of Nexperion. He might be able to help.
His email address: guenter.resch-at-nexperion.net

Cheers,

Jan Leunissen

} On 26/01/2017, at 21:46, rongchigram79-at-yahoo.com.sg wrote:
}
}
}
}
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} Dear All, i would like to ask for your recommendation for a temperature logger. Probably a reasonable sensitivity, acuracy and precision and is able to monitor live using the TEM pc. I am not sure if such system will interfere any performance of the TEM. If you encounter such problem, is it possible to share your experience? I do know some logger has wifi/bluetooth capability, will this interfere the TEM performances well? whiCurrently we have a temperature logger but its not live and we have to extract the data out from time to time which is very troublesome.
}
} Cheers,
} Yee Yan, Tay
} FACTS Lab
} NTU
} Singapore
}
} ==============================Original Headers==============================
} 2, 29 -- From rongchigram79-at-yahoo.com.sg Thu Jan 26 02:46:34 2017
} 2, 29 -- Received: from nm11-vm7.bullet.mail.sg3.yahoo.com (nm11-vm7.bullet.mail.sg3.yahoo.com [106.10.148.246])
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} 2, 29 -- From: YY YY {rongchigram79-at-yahoo.com.sg}
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From: vray-at-partbeamsystech.com
Date: Thu, 26 Jan 2017 10:45:11 -0600
Subject: [Microscopy] Fwd: Temperature Logger to Recommend

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have used Extech RH520A temperature/humidity data logger in FIB room
and was very pleased with it in all respects. Initial setup required
some programming, but features and display/download capabilities were
excellent:

http://www.extech.com/display/?id=14702

Valery Ray
www.linkedin.com/in/valeryray {http://www.linkedin.com/in/valeryray}
==============================
PBS&T, MEO Engineering Company
290 Broadway, Suite 298
Methuen, MA 01844, USA
Phone: +1-978-305-0479 {tel:%2B1-978-305-0479} - leave a message
Mobie: +1-978-305-0479 {tel:%2B1-978-305-0479} - leave a message
E-mail: vray-at-partbeamsystech.com {mailto:vray-at-partbeamsystech.com}
Web: www.partbeamsystech.com {http://www.partbeamsystech.com}
Web: www.freudlabs.com {http://www.freudlabs.com}


On 1/26/2017 4:06 AM, leunissen-at-aurion.nl wrote:
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} Hello,
}
} you may want to talk to Guenter Resch of Nexperion. He might be able to help.
} His email address:guenter.resch-at-nexperion.net
}
} Cheers,
}
} Jan Leunissen
}
} } On 26/01/2017, at 21:46,rongchigram79-at-yahoo.com.sg wrote:
} }
} }
} }
} }
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} }
} } Dear All, i would like to ask for your recommendation for a temperature logger. Probably a reasonable sensitivity, acuracy and precision and is able to monitor live using the TEM pc. I am not sure if such system will interfere any performance of the TEM. If you encounter such problem, is it possible to share your experience? I do know some logger has wifi/bluetooth capability, will this interfere the TEM performances well? whiCurrently we have a temperature logger but its not live and we have to extract the data out from time to time which is very troublesome.
} }
} } Cheers,
} } Yee Yan, Tay
} } FACTS Lab
} } NTU
} } Singapore
} }
} } ==============================Original Headers==============================
} } 2, 29 -- Fromrongchigram79-at-yahoo.com.sg Thu Jan 26 02:46:34 2017
} } 2, 29 -- Received: from nm11-vm7.bullet.mail.sg3.yahoo.com (nm11-vm7.bullet.mail.sg3.yahoo.com [106.10.148.246])
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} } 2, 29 -- From: YY YY {rongchigram79-at-yahoo.com.sg}
} } 2, 29 -- Reply-To: YY YY {rongchigram79-at-yahoo.com.sg}
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} 7, 35 -- Fromleunissen-at-aurion.nl Thu Jan 26 03:04:45 2017
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From: John.Mardinly-at-asu.edu
Date: Fri, 27 Jan 2017 10:30:47 -0600
Subject: [Microscopy] Re: Temperature Logger to Recommend

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello
Anybody knows what are the sizes of the 3 holes of the strip aperture of the
Jeol 5600LV?
Thanks for your time!
yorgos

Dr Yorgos Nikas
Athens Innovative Microscopy
Skra 36 Voula 16673 GREECE

Tel/fax +30 210 8957677
mobile +30 6945 107477
www.eikonika.netwww.aim.cat
*************************************







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8, 20 -- From eikonika-at-otenet.gr Fri Jan 27 03:23:23 2017
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From annepenn4-at-gmail.com Fri Jan 27 06:05:02 2017
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} I recommend a temperature sensor from Vernier.com. Their line of Go Direct sensors connect to a laptop by USB, are dirt cheap ($39 for the one I have), and come with a very nice free software package (I have never seen it crash!) for recording your measurements. I see now they have options for wireless with iOS devices. Have fun!
}
}
}
} John Mardinly, Ph.D., P.E., Retired ASU
}
}
}
} } On Jan 26, 2017, at 1:59 AM, rongchigram79-at-yahoo.com.sg wrote:
} }
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} }
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} } Dear All, i would like to ask for your recommendation for a temperature logger. Probably a reasonable sensitivity, acuracy and precision and is able to monitor live using the TEM pc. I am not sure if such system will interfere any performance of the TEM. If you encounter such problem, is it possible to share your experience? I do know some logger has wifi/bluetooth capability, will this interfere the TEM performances well? whiCurrently we have a temperature logger but its not live and we have to extract the data out from time to time which is very troublesome.
} }
} } Cheers,
} } Yee Yan, Tay
} } FACTS Lab
} } NTU
} } Singapore
} }
} } ==============================Original Headers==============================
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From: WHITTAKS-at-si.edu
Date: Fri, 27 Jan 2017 10:42:14 -0600
Subject: [Microscopy] Re: Temperature Logger to Recommend

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have several units from ITWatchdogs. Never had any problem and they email and text me and the building managers when there is a thermal issue out of range. Interface is a standard web browser.

Scott Whittaker
Lab Manager
Imaging
P.O. Box 37012 MRC-104 Room W150
Washington, DC 20013-7012
w 202.633.0891 whittaks-at-si.edu

SMITHSONIAN INSTITUTION
NATIONAL MUSEUM OF NATURAL HISTORY



-----Original Message-----
X-from: John.Mardinly-at-asu.edu [mailto:John.Mardinly-at-asu.edu]
Sent: Friday, January 27, 2017 11:38 AM
To: Whittaker, Scott {WHITTAKS-at-si.edu}


} I recommend a temperature sensor from Vernier.com. Their line of Go Direct sensors connect to a laptop by USB, are dirt cheap ($39 for the one I have), and come with a very nice free software package (I have never seen it crash!) for recording your measurements. I see now they have options for wireless with iOS devices. Have fun!
}
}
}
} John Mardinly, Ph.D., P.E., Retired ASU
}
}
}
} } On Jan 26, 2017, at 1:59 AM, rongchigram79-at-yahoo.com.sg wrote:
} }
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} }
} } Dear All, i would like to ask for your recommendation for a temperature logger. Probably a reasonable sensitivity, acuracy and precision and is able to monitor live using the TEM pc. I am not sure if such system will interfere any performance of the TEM. If you encounter such problem, is it possible to share your experience? I do know some logger has wifi/bluetooth capability, will this interfere the TEM performances well? whiCurrently we have a temperature logger but its not live and we have to extract the data out from time to time which is very troublesome.
} }
} } Cheers,
} } Yee Yan, Tay
} } FACTS Lab
} } NTU
} } Singapore
} }
} } ==============================Original
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From: microscopy.listserver-at-gmail.com
Date: Tue, 31 Jan 2017 07:37:58 -0600
Subject: [Microscopy] viaWWW:HA tag - live imaging

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Email: stirling.newberry-at-gmail.com Name: Stirling Newberry

Organization: MIT

Title-Subject: [Filtered] Sterling P Newberry

Message: Colleagues, we regret to note the passing of
Sterling Newberry a long time member of the microscopy
community whose passion for microscopy and education
will be missed.


https://en.wikipedia.org/wiki/Sterling_Newberry

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To: microscopylistserverarchive6-at-microscopy.com

Thanks to all who responded. We have found a new home for this
system. If arrangements fall through, we will select another choice
from the previous inquiries.

Regards,
Jim

--
Jim Passmore
Principal Scientist, R&D

Sealed Air Corporation
P: 864.433.2927
100 Rogers Bridge Rd., Bldg A
Duncan, SC 29334

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From smithdiana157-at-gmail.com Mon Jan 30 11:59:01 2017
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X-from: leandro.lemgrubersoares-at-glasgow.ac.uk

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Email: leandro.lemgrubersoares-at-glasgow.ac.uk Name: Leandro Lemgruber

Organization: University of Glasgow

Title-Subject: [Filtered] HA tag - live imaging

Message: Dear All,
does anyone know if there is a membrane permeable dye or probe that recognises HA-tag protein in
live imaging?

Thanks!

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From: henning.stahlberg-at-unibas.ch
Date: Tue, 31 Jan 2017 14:38:41 -0600
Subject: [Microscopy] Fwd: [3dem] GRC Locations

Contents Retrieved from Microscopy Listserver Archives
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} Begin forwarded message:
}
} From: Nancy Gray {ngray-at-grc.org}
} Subject: Re: [3dem] GRC Locations
} Date: January 31, 2017 at 19:19:24 GMT+1
} To: "Smith, Phillip R." {smithp01-at-nyumc.org} , Marin van Heel {marin.vanheel-at-googlemail.com}
} Cc: Henning Stahlberg {henning.stahlberg-at-unibas.ch} , Microscopy {Microscopy-at-microscopy.com} , 3dem {3dem-at-ncmir.ucsd.edu} , "CCPEM-at-JISCMAIL.AC.UK" {CCPEM-at-JISCMAIL.AC.UK}
}
} Folks,
} Could you kindly forward my response to your network since my attempt was rejected since I am not authorized. I am getting many emails on the issue and would like all to know GRC is formulating a response. Many thanks,
} Nancy
}
} Nancy Ryan Gray, PhD
} Gordon Research Conferences, President and Chief Executive Officer
} 512 Liberty Lane
} West Kingston, RI 02892
} 401-360-1521
} www.grc.org
}
}
} } On Jan 31, 2017, at 11:33 AM, Nancy Gray {ngray-at-grc.org} wrote:
} }
} } Dear Henning, Ulrike, Stefan, Marin, Ross and members of the 3dem mailing list,
} }
} } Thank you for your input, it is indeed a difficult situation. I have scheduled a conference call with the GRC Board of Trustees Executive Committee and GRC Legal Counsel to address these concerns and will get back to you shortly.
} } Thanks for your support, patience and understanding.
} } Nancy
} }
} }
} }
} } Nancy Ryan Gray, PhD, President and Chief Executive Officer
} } Gordon Research Conferences
} } 512 Liberty Lane, West Kingston, RI 02892-1502
} } Phone: 401-360-1521 l Fax: 401-783-7644
} } Email: ngray-at-grc.org l Web: http://www.grc.org

[]


} } } From: Henning Stahlberg {henning.stahlberg-at-unibas.ch}
} } } Subject: [3dem] GRC Locations
} } } Date: January 30, 2017 at 21:08:10 GMT+1
} } } To: "Nancy R. Gray" {NGray-at-grc.org}
} } } Cc: Microscopy {Microscopy-at-microscopy.com} , 3dem {3dem-at-ncmir.ucsd.edu}
} } }
} } } Dear Nancy,
} } }
} } } Science is open and international. The US is currently not, unfortunately.
} } } Until that situation is resolved, may I ask you to consider moving the upcoming GRC conferences to openly accessible locations, such as international conference sites as in Cancun in Mexico or in other countries.
} } } This would allow fair and equal access of scientist from all countries and religions to your conferences, and prevent a boycott movement from forming.
} } } Your conferences in Europe and Asia are a great start.
} } }
} } } With best wishes,
} } }
} } } Henning Stahlberg.
} } }
} } }
} } } Henning Stahlberg, PhD
} } } Prof. for Structural Biology, C-CINA, Biozentrum, University Basel
} } } Mattenstrasse 26 | D-BSSE | WRO-1058 | CH-4058 Basel | Switzerland
} } } http://c-cina.org | Tel. +41-61-387 32 62
} } }


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From: microscopy.listserver-at-gmail.com
Date: Tue, 31 Jan 2017 16:58:43 -0600
Subject: [Microscopy] viaWWW:Histo / EM position open

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X-from: erosen-at-mednet.ucla.edu

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Email: erosen-at-mednet.ucla.edu Name: Eric Rosen
Organization: UCLA Medical Center
Title-Subject: [Filtered] Histo / EM position open
Message: Hi all,

We have a 50% Histo/50% EM position open at UCLA hospital.

See job description here:
http://www.uclahealthcareers.org/all-jobs/Histotechnologist-II/H88333
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From: jacques.faerber-at-ipcms.unistra.fr
Date: Wed, 1 Feb 2017 03:19:35 -0600
Subject: [Microscopy] EDAX .spc to .emsa format batch conversion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all

One day too late, but anyway happy new year to all, as it is my first
post of the year !

I'm looking if someone could have soon written a bash script (or else
working on linux system) to convert in batch mode EDAX spectra from the
.spc file format to .emsa/msa.
The "Export" function of the TEAM software has only the .spc format as
output, and the "Send_To_Folder" function is far too time consuming for
saving a project part with tenth of spectra, in particular as it doesn't
keep the original names of the data.

I tried Spectrum Viewer but I didn't bring it to work on linux (via
Wine, but I'm very binary with Wine : it works or doesn't work... I've
no time to learn to configure it !).

Fortunately, DTSA-II reads the .spc format and can save in emsa, what
allows me to work. But DTSA has not the goal to do batch format
conversions !

Thanks to all

Jacques

J. Faerber
IPCMS-DSI
Institut de Physique et Chimie des Matériaux de Strasbourg
Département Surfaces et Interfaces
23, rue de Loess ; BP43
67034 Strasbourg CEDEX 2
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)3 88 10 72 48
E-mail : Jacques.Faerber-at-ipcms.unistra.fr


==============================Original Headers==============================
10, 26 -- From jacques.faerber-at-ipcms.unistra.fr Wed Feb 1 03:19:35 2017
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From: joshua.taillon-at-nist.gov
Date: Wed, 1 Feb 2017 08:39:38 -0600
Subject: [Microscopy] EDAX .spc to .emsa format batch conversion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Jacques,

The open-source HyperSpy software (www.hyperspy.org) can do what you are looking for.
EDAX file reading (both .spd and .spc) has been added in the latest development builds (and should be included in the next minor release -- v1.2). If you cannot wait for that, setting up a copy of the development version isn't too difficult if you have some proficiency with Python and git (http://hyperspy.org/hyperspy-doc/current/user_guide/install.html#development-version).

Good luck!

- Josh

NB: Any mention of commercial products or software is for information only; it does not imply recommendation or endorsement by NIST.

--
Joshua Taillon, Ph.D.
Materials Measurement Science Division
National Institute of Standards and Technology

100 Bureau Drive, MS-8372
Gaithersburg, MD 20899
W: (301) 975-2913
E: joshua.taillon-at-nist.gov



-----Original Message-----
X-from: jacques.faerber-at-ipcms.unistra.fr [mailto:jacques.faerber-at-ipcms.unistra.fr]
Sent: Wednesday, February 1, 2017 4:42 AM
To: Taillon, Joshua A. (Fed) {joshua.taillon-at-nist.gov}

Hi all

One day too late, but anyway happy new year to all, as it is my first post of the year !

I'm looking if someone could have soon written a bash script (or else working on linux system) to convert in batch mode EDAX spectra from the .spc file format to .emsa/msa.
The "Export" function of the TEAM software has only the .spc format as output, and the "Send_To_Folder" function is far too time consuming for saving a project part with tenth of spectra, in particular as it doesn't keep the original names of the data.

I tried Spectrum Viewer but I didn't bring it to work on linux (via Wine, but I'm very binary with Wine : it works or doesn't work... I've no time to learn to configure it !).

Fortunately, DTSA-II reads the .spc format and can save in emsa, what allows me to work. But DTSA has not the goal to do batch format conversions !

Thanks to all

Jacques

J. Faerber
IPCMS-DSI
Institut de Physique et Chimie des Matériaux de Strasbourg Département Surfaces et Interfaces 23, rue de Loess ; BP43
67034 Strasbourg CEDEX 2
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)3 88 10 72 48
E-mail : Jacques.Faerber-at-ipcms.unistra.fr


==============================Original Headers==============================
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==============================Original Headers==============================
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26, 56 -- From: "Taillon, Joshua A. (Fed)" {joshua.taillon-at-nist.gov}
26, 56 -- To: "jacques.faerber-at-ipcms.unistra.fr" {jacques.faerber-at-ipcms.unistra.fr}
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26, 56 -- Subject: RE: [Microscopy] EDAX .spc to .emsa format batch conversion
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From: vray-at-partbeamsystech.com
Date: Wed, 1 Feb 2017 10:28:12 -0600
Subject: [Microscopy] Re: EDAX .spc to .emsa format batch conversion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Jacques,

Unless someone have already written and would share the script you need
for converting .spc to .emsa, it should be possible to automate
sequential opening of files within a folder by DTSA-II, saving data in
another format, and repeating the operation until all files are
processed with one of GUI scripting tools. I am using WinBatch (US$100
from http://www.windowware.com/, no connection just a happy user) for
file processing purposes, although in different then yours application.
There is also AutoIt which is free from www.autoitscript.com, but I did
not try it. Not aware of similar GUI scripting tools for Linux, but
would be surprised if they didn't exist as well.

Valery Ray
www.linkedin.com/in/valeryray
==============================
PBS&T, MEO Engineering Company
290 Broadway, Suite 298
Methuen, MA 01844, USA
Phone: +1-978-305-0479 - leave a message
Mobie: +1-978-305-0479 - leave a message
E-mail: vray-at-partbeamsystech.com
Web: www.partbeamsystech.com
Web: www.freudlabs.com

On 2/1/2017 4:20 AM, jacques.faerber-at-ipcms.unistra.fr wrote:
} ----------------------------------------------------------------------------
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}
} Hi all
}
} One day too late, but anyway happy new year to all, as it is my first
} post of the year !
}
} I'm looking if someone could have soon written a bash script (or else
} working on linux system) to convert in batch mode EDAX spectra from the
} .spc file format to .emsa/msa.
} The "Export" function of the TEAM software has only the .spc format as
} output, and the "Send_To_Folder" function is far too time consuming for
} saving a project part with tenth of spectra, in particular as it doesn't
} keep the original names of the data.
}
} I tried Spectrum Viewer but I didn't bring it to work on linux (via
} Wine, but I'm very binary with Wine : it works or doesn't work... I've
} no time to learn to configure it !).
}
} Fortunately, DTSA-II reads the .spc format and can save in emsa, what
} allows me to work. But DTSA has not the goal to do batch format
} conversions !
}
} Thanks to all
}
} Jacques
}
} J. Faerber
} IPCMS-DSI
} Institut de Physique et Chimie des Matériaux de Strasbourg
} Département Surfaces et Interfaces
} 23, rue de Loess ; BP43
} 67034 Strasbourg CEDEX 2
} France
}
} Tel 00 33(0)3 88 10 71 01
} Fax 00 33(0)3 88 10 72 48
} E-mail : Jacques.Faerber-at-ipcms.unistra.fr
}
}
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4, 37 -- References: {201702010920.v119KpQx020159-at-microscopy.com}
4, 37 -- From: Valery Ray {vray-at-partbeamsystech.com}
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From: jrminter-at-gmail.com
Date: Wed, 1 Feb 2017 12:00:07 -0600
Subject: [Microscopy] 1 of 2 Print all In new window RE: EDAX .spc to msa spectrum conversion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Jacques Faerber asked about a script for the conversion of EDAX .spc to msa
format.

DTSA-II has a nice scripting language (jython) that permits this. One can
also apply a DTSA calibrated detector instance to the spectrum. I sent Jacques
amd earlier version of this this morning. I want to make certain that the
formatting stays correct, so I created a public gist on GitHub. You can
obtain the script at:

https://gist.github.com/jrminter/a697f83bec3f37dfb824fb7126542c41


Hope this helps.

John Minter

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From: frank_karl-at-ardl.com
Date: Wed, 1 Feb 2017 14:04:53 -0600
Subject: [Microscopy] Our TEM needs a cool breeze.....

Contents Retrieved from Microscopy Listserver Archives
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Hello Everyone,
Our 1 meg Gatan Camera (model 780 Duel vision) on our TEM is dying and we want to give it a transplant. I'm looking for a cooling fan.

It's the 780.PL6FA model, if that helps. It's from the turn of the century and if you have a one that's broken and the fan works or you have a spare fan or know of a replacement fan, I sure would like to hear from you.

Thanks in advance........
Frank Karl
ARDL
330-794-6600

This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc.



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From: mikepidg-at-gmail.com
Date: Wed, 1 Feb 2017 23:11:28 -0600
Subject: [Microscopy] job search

Contents Retrieved from Microscopy Listserver Archives
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I am an accomplished Electron Microscopy Technician, supervisor, and
research specialist with 25 years experience working in both clinical
and research environments looking for a position where my acquired
skills can be used for professional advancement.

Thank you,

Michael Pidgeon

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From: microscopy.listserver-at-gmail.com
Date: Wed, 1 Feb 2017 23:22:41 -0600
Subject: [Microscopy] Administrivia: Please follow the ML Rules

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues:

Can I remind everyone about the Microscopy Listserver Rules which can be found at:

http://www.microscopy.com/MicroscopyListserver/FAQ.html

Posting of "looking for a position" emails has never been permitted. Please
do not do this.

There are job boards, one specifically hosted and run by the Microscopy Society
of America http://jobs.microscopy.org/ that fulfill this role.


Nestor
Your Friendly Neighborhood SysCop


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From: microscopy.listserver-at-gmail.com
Date: Thu, 2 Feb 2017 06:53:17 -0600
Subject: [Microscopy] viaWWW:Jeol strip aperture sizes

Contents Retrieved from Microscopy Listserver Archives
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X-from: nlamine6-at-hotmail.com

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MLFormMail.html
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Email: nlamine6-at-hotmail.com Name: naitbouda

Organization: cdta

Title-Subject: [Filtered] strip

Message: Hello

hello the size of jeol 5600LV and JEOL 6360LV
IS THE SAME the Jeol strip apertures 3 holes are/
20micron -30um-100um

you can use the size of
Jeol strip aperture 9.0x2.35x0.10mm (platinium-Iridium)

Part:67001-10-20-100


best regards
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From: rjpalmer-at-dir.nidcr.nih.gov
Date: Thu, 2 Feb 2017 07:48:41 -0600
Subject: [Microscopy] FW: Fwd: [3dem] GRC Locations

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Dear Ms. Ryan -

Thank you for your efforts to disseminate your communications to a larger
audience. This is an important issue from many perspectives and I imagine
most subscribers to this list will be very interested in how the GRC
responds. I would suggest that scientific societies all ought to have a
measured and well thought out position on the current political situation,
and that those positions be made abundantly clear world-wide. From my
personal perspective, I feel boycotts are unhelpful in resolving larger
issues, especially when the boycotting group has little to no true
leverage other than to shoot itself in the foot.

Robert J. Palmer Jr., PhD
National Institute of Dental and Craniofacial Research
National Institutes of Health
Building 30, Room 331
Bethesda MD 20892
301-594-0025




On 1/31/17, 4:00 PM, "henning.stahlberg-at-unibas.ch"
{henning.stahlberg-at-unibas.ch} wrote:

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From: microscopy.listserver-at-gmail.com
Date: Thu, 2 Feb 2017 11:24:15 -0600
Subject: [Microscopy] viaWWW:Leica Sales Opportunity - Southeast USA

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Email: lon.nelson-at-leica-microsystems.com Name: Lon Nelson

Organization: Leica Microsystems

Title-Subject: [Filtered] Leica Sales Agent / Dealer Opportunity - Southeast USA

Message: Leica Microsystems is currently seeking a commissioned Sales Agent or Dealer in the
Southeast USA for our Electron Microscopy Sample Preparation solutions.

https://tinyurl.com/leicaEMprep

Interested parties should contact Lon Nelson, Director of Sales, at lon.nelson-at-leica-microsystems.com.

Best regards,

Lon Nelson
Director of Sales – Microscopy
lon.nelson-at-leica-microsystems.com
M +1 224-628-2467 | F +1 847-607-3160
Leica Microsystems, Inc.
1700 Leider Ln | Buffalo Grove, IL 60089 (USA)


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From: ALawrence-at-i2at.msstate.edu
Date: Thu, 2 Feb 2017 11:28:23 -0600
Subject: [Microscopy] M&M 2017 meeting - Student Bursaries

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The deadline for paper submission for M&M 2017 is less than two weeks away and as you are making plans to attend the St.
Louis meetings (Aug. 6-10), please don't forget about MSA's student bursary program. Its purpose is to encourage students to
attend the meetings by helping to defray some of the costs while giving them an opportunity to meet and interact with the
established microscopy community.

The student bursaries will be paid $10 an hour to work for ~20 hours during the meeting or pre-meeting events. The jobs involve
such things as providing support in the different symposia, staffing the volunteer office; newsletter distribution, and helping
with vendor tutorial sign-up.

Once the program has been finalized, each registered bursary will be contacted and given the chance to choose the times and
activities where they would like to help. There is an added bonus of $10 cash for each morning and/or afternoon session worked
to assist with meals and a meeting shirt.

If anyone would like to participate in the bursary program, please check the box "I wish to apply for a student bursary" in section
2 of the registration form. Applicants for the bursaries must be members of MSA or MAS, enrolled as students at a recognized
educational institution, and pay their registration fee. Bursary space is limited, so sign-up early.

If anyone has any questions about the bursary program, or would like to participate, please contact Amanda
(alawrence-at-i2at.msstate.edu).

Also don't forget about the opportunities for meeting awards!


Amanda Lawrence
Institute for Imaging and Analytical Technologies
Mississippi State University
662-325-7998
alawrence-at-i2at.msstate.edu


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9, 32 -- Subject: M&M 2017 meeting - Student Bursaries
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From: zaluzec-at-microscopy.com
Date: Thu, 2 Feb 2017 16:09:59 -0600
Subject: [Microscopy] viaWWW:M&M2017 - Symposium A09 Standards, Reference Materials, and

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: owen.neill-at-wsu.edu
To: Zaluzec-at-microscopy.com

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Email: owen.neill-at-wsu.edu Name: Owen Neill

Organization: Peter Hooper GeoAnalytical Lab, Washington State University

Title-Subject: [Filtered] M&M2017 - Symposium A09

Message: Dear colleagues,
Apologies for the cross-postings, but I hope 2017 is off to a good
start for everyone. Paper submission for the Microscopy and
Microanalysis 2017 Annual Meeting (St. Louis, MO, 6-10 August, 2017) is
now open, and will close on 15 February. My co-conveners and I would
like to draw your attention to:
Symposium A09, Standards, Reference Materials, and Their Applications
in Quantitative Microanalysis.
We are looking for submissions dealing with the synthesis, evaluation,
and need for new reference materials; evaluation, distribution, and
maintenance of existing reference materials; the use of standards in
quantitative microanalysis; and the application of quantitative
microanalytical techniques to solving analytical problems. A full
description of this session is included below. Papers may be submitted
via the M&M2017 website: http://www.microscopy.org/mandm/2017/
We are also pleased to announce the invited speakers for this symposium:
• John Hanchar, Department of Earth Sciences, Memorial University,
Newfoundland
• William Nachlas, Department of Earth Sciences, Syracuse University
• Timothy Rose, Department of Mineral Sciences, Smithsonian Institution
• Stephen Wilson, Reference Materials Program, United States Geological
Survey
We are very excited to hear from these experts in microanalytical
standards and quantitative microanalysis, and we look forward to hearing
about your work as well. See you in St. Louis!
The organizers of Symposium A09:
Julien Allaz, University of Colorado – Boulder
Anette von der Handt, University of Minnesota – Twin Cities
Owen Neill, Washington State University
Symposium Description:
Standards and reference materials are essential for obtaining accurate
quantitative compositional data from X-ray microanalysis by EPMA or SEM
(WDS/EDS), as well as from other microanalytical techniques (LA-ICP-MS,
SIMS, µ-XRF, FTIR, Raman spectroscopy, etc.). These materials must be
rigorously evaluated for their reference compositions and homogeneity,
must be widely available to the analytical community, and must be
properly maintained to avoid contamination or deterioration. We welcome
contributions on the synthesis, evaluation, distribution, and
maintenance of standards and reference materials, as well as their
appropriate use in microanalysis. We further encourage submissions on
standard-based applications of quantitative microanalysis, or on the
development of new quantitative microanalytical protocols.

Topics of interest include:
• The use of standards and reference materials in quantitative
microanalysis, and the needs of the analytical community for improving
such materials.
• Synthesis, evaluation, distribution, and maintenance of standards and
reference materials.
• Development of new protocols for microanalytical techniques. •
Applications of standard-based techniques to solving microanalytical
problems.


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From: microscopy.listserver-at-gmail.com
Date: Fri, 3 Feb 2017 22:38:57 -0600
Subject: [Microscopy] viaWWW: Need Gatan 794 Camera Controller

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Email: jhyun-at-gatan.com Name: John Hyun

Organization: Gatan

Title-Subject: [Filtered] dvanced EELS and EFTEM Training School -
France 2017

Message: The Advanced EELS and EFTEM Training School hosted by EDF R&D
is an intensive 4-day training school that incorporates lectures,
computer laboratories, and microscope practical classes to provide
participants with a comprehensive, hands-on training on advanced
electron energy loss spectroscopy (EELS) and energy-filtered
transmission electron microscopy (EFTEM) topics.

The practical sessions on the microscope will be given at the Materials
Ageing Institute. This course focuses on the advanced practice of EELS
based imaging and analysis in the (S)TEM microscope on advanced based
EELS techniques.

A good experience with electron microscopy and EELS is highly
recommended. By the end of the course, participants can expect to get
the most out of their EELS spectrometer system and know how to best
optimize the experimental conditions in order to capture the maximum
amount of information out of the TEM samples using EELS.

Online information and registration:
http://www.gatan.com/company/events/advanced-eels-and-eftem-training-school-france-2017


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From anittiff04-at-gmail.com Thu Feb 2 16:11:29 2017
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Email: jwsandinod-at-unal.edu.co Name: John W. Sandino

Organization: Universidad Nacional de Colombia

Title-Subject: [Filtered] Need Gatan 794 Camera Control

Message: Two years ago we received an old 794 retractable camera Gatan
as donation from the Tribenberg Lab (closed).
But two days ago the camera controller of this camera does not work any
more. Today the gatan people confirm the news and told as that the
solution would buy a new camera. However we don't have budget for that.

We ask for some who could have a camara contoller that coud give us for
free.
I know that it is no the best petition but for the moment is the unique
solution to work with our tecnai 20. one of the best microscopes in our
country Colombia.

Thanks
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From: microscopy.listserver-at-gmail.com
Date: Fri, 3 Feb 2017 22:43:21 -0600
Subject: [Microscopy] viaWWW:mmc2017 and EMAG 2017 Abstract Submission Deadline - 17

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Email: mreedman-at-rms.org.uk Name: Mel Reedman

Organization: Royal Microscopical Society

Title-Subject: [Filtered] mmc2017 Abstract Deadline Approaching

Message: mmc2017 and EMAG 2017 Abstract Submission Deadline - 17 February
---------------------------------------------------

The Microscience Microscopy Congress is returning to Manchester from 3 -
6 July 2017 and now is the time to join us at this great event by
submitting an abstract!

www.mmc-series.org.uk/abstracts
There is only 2 weeks left to submit an oral abstract as submission
closes on Friday 17 February 2017.

The Call for Papers covers a wide variety of topics and techniques as
you can see below:

Life Sciences Sessions
- Bio Applications: Investigating Biological Structure using Electron
Tomography
- Imaging in Flow Cytometry
- Imaging protein dynamics in living cells
- Investigating Biological Structure using Electron Tomography
- Bio Applications: Imaging Cells in 3D - matrix, tissue, in vivo
- Bio EM Sample Preparation and Analysis
- Bio Applications: Imaging Cancer
- Correlative Microscopy
- Bio-Applications: Long-term imaging using Single Plane Illumination
Microscopy
- Biological Processes at the Nanoscale (Celtic Session organised by MSI
and SMG)
- Microbial Imaging
- Host-Pathogen Interactions
- Bio Applications: Bacterial Ultrastructure
- Electron Cryomicroscopy of Biological Macromolecules
- Biological Applications of 3D Electron Microscopy
Frontiers in Bioimaging Sessions
- Bespoke light microscopy for molecular level imaging
- Advances in labelling for super-resolution microscopy
- Advanced light imaging for addressing longer length scale biological
questions
- Developing super-resolution methods for functional insight

Physical Sciences Sessions
- Earth, Environmental and Planetary Materials
- Microscopy of Engineered Surfaces and Tribology
- Energy and Energy Storage Materials
- Inorganic Nanomaterials (Celtic Session organised by MSI and SMG)

EMAG 2017 Keywords
Instrumentation
- 3-D microscopy
- in-situ microscopy techniques
- Low Dose Imaging and Analytical Microscopy
- Dynamic TEM
- Advances in SEM & FIB (CL, EBSD, beam deceleration, monochromators etc)
- Detector technologies and new instrumentation
- Vortex Beams and Phase plates
- Electron crystallography and diffraction
Materials
- Biological materials, Biomaterials and Soft matter
- Geological microscopy
- Catalytic materials
- Nanomaterials and 2D materials
- Energy and Energy Storage Materials
- Functional Materials
- Structural Materials and Metallurgy
- Surface imaging and modification
- Microscopy of interfaces and heterostructures
- High spatial resolution chemical and structural analysis

Scanning Probe Microscopy Sessions
- Frontiers of Scanning Probe Microscopy
- High Resolution SPM
- Structures, Interfaces and Mechanics in Life and Health with AFM
- SPM nano-mapping of materials properties

Not sure where your work would fit? You can view further details about
each conference session at
www.mmc-series.org.uk/conference/conference-sessions
Submit your abstract at www.mmc-series.org.uk/abstracts
Conference Registration is now open, book your place at
www.mmc-series.org.uk/registration

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From: microscopy.listserver-at-gmail.com
Date: Fri, 3 Feb 2017 22:44:09 -0600
Subject: [Microscopy] viaWWW:Oxford INCA EDS detector dead time 100%

Contents Retrieved from Microscopy Listserver Archives
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Email: hexi-at-missouri.edu Name: Xiaoqing He

Organization: Universtiy of Missouri

Title-Subject: [Filtered] Oxford INCA EDS detector dead time 100%

Message: Hi all,

We are having issues with our Oxford EDS detector on our F30. With no
electron beam on, the dead time stayed at 100% no matter which
dispersion rate we choose. Initially we thought the misconnection
between Oxford and TEM may be responsible. But the issue persists even
after we reboot Microscope PC, Oxford x-stream processor, software. etc.

Any inputs are greatly appreciated!

Thanks.

Xiaoqing He

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From: vray-at-partbeamsystech.com
Date: Sat, 4 Feb 2017 07:32:13 -0600
Subject: [Microscopy] Re: viaWWW: Need Gatan 794 Camera Controller

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi John,

One Gatan 794 controller is listed on E*Bay and the seller is willing to
ship to Colombia - this is not free, but certainly less expensive then a
new camera:

http://www.ebay.com/itm/GATAN-Retractable-Multiscan-Camera-Controller-Model-794-/361863685735?hash=item5440c1a667:g:K5cAAOSw5cNYYejS#shpCntId

Look really hard for local people who do component level repair of
complex electronics - if the problem is in controller and not the
camera, then most likely it is repairable! You just need to find someone
capable of such repair and willing to do it for the money you are able
to pay...

Best Wishes,
Valery

Valery Ray
www.linkedin.com/in/valeryray
==============================
PBS&T, MEO Engineering Company
290 Broadway, Suite 298
Methuen, MA 01844, USA
Phone: +1-978-305-0479 - leave a message
Mobie: +1-978-305-0479 - leave a message
E-mail: vray-at-partbeamsystech.com
Web: www.partbeamsystech.com
Web: www.freudlabs.com

On 2/3/2017 11:40 PM, microscopy.listserver-at-gmail.com wrote:
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} Email: jwsandinod-at-unal.edu.co Name: John W. Sandino
}
} Organization: Universidad Nacional de Colombia
}
} Title-Subject: [Filtered] Need Gatan 794 Camera Control
}
} Message: Two years ago we received an old 794 retractable camera Gatan
} as donation from the Tribenberg Lab (closed).
} But two days ago the camera controller of this camera does not work any
} more. Today the gatan people confirm the news and told as that the
} solution would buy a new camera. However we don't have budget for that.
}
} We ask for some who could have a camara contoller that coud give us for
} free.
} I know that it is no the best petition but for the moment is the unique
} solution to work with our tecnai 20. one of the best microscopes in our
} country Colombia.
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From: vray-at-partbeamsystech.com
Date: Wed, 8 Feb 2017 11:38:15 -0600
Subject: [Microscopy] JEOL JEM 2100F installation/service manual?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All, thank you very much for those who have kindly made some recommendations! I have summarized here the ones that have been recommended


1. Günter Resch (Nexperion, Vienna)
2. TipTemp
3. Extech RH520A temperature/humidity data logger
4. Vernier.com, Go Direct sensors

Hope this will be useful for the rest as well!

Cheers,
Yee Yan




On Thursday, 26 January 2017, 16:51, YY YY {rongchigram79-at-yahoo.com.sg} wrote:



Dear All, i would like to ask for your recommendation for a temperature logger. Probably a reasonable sensitivity, acuracy and precision and is able to monitor live using the TEM pc. I am not sure if such system will interfere any performance of the TEM. If you encounter such problem, is it possible to share your experience? I do know some logger has wifi/bluetooth capability, will this interfere the TEM performances well? whiCurrently we have a temperature logger but its not live and we have to extract the data out from time to time which is very troublesome.

Cheers,
Yee Yan, Tay
FACTS Lab
NTU
Singapore


==============================Original Headers==============================
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14, 31 -- From: YY YY {rongchigram79-at-yahoo.com.sg}
14, 31 -- Reply-To: YY YY {rongchigram79-at-yahoo.com.sg}
14, 31 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
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14, 31 -- Subject: Re: Temperature Logger to Recommend
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From stepnoah502-at-gmail.com Sun Feb 5 10:47:09 2017
Return-Path: {stepnoah502-at-gmail.com}
Received: from gmail.com ([61.76.233.68])
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Message-ID: {F404FFFA.5BD98938-at-gmail.com}

http://www.practicaldesign.com/THUM/thum.html

I have used two of these for over a decade to monitor T/H in microscope rooms.
USB interface, so PC should be on, and data stored in a database.

Cheers,

Fred Monson

-----Original Message-----
X-from: rongchigram79-at-yahoo.com.sg [mailto:rongchigram79-at-yahoo.com.sg]
Sent: Saturday, February 04, 2017 10:59 AM
To: Monson, Frederick {FMonson-at-wcupa.edu}

Dear All, thank you very much for those who have kindly made some recommendations! I have summarized here the ones that have been recommended


1. Günter Resch (Nexperion, Vienna)
2. TipTemp
3. Extech RH520A temperature/humidity data logger
4. Vernier.com, Go Direct sensors

Hope this will be useful for the rest as well!

Cheers,
Yee Yan




On Thursday, 26 January 2017, 16:51, YY YY {rongchigram79-at-yahoo.com.sg} wrote:



Dear All, i would like to ask for your recommendation for a temperature logger. Probably a reasonable sensitivity, acuracy and precision and is able to monitor live using the TEM pc. I am not sure if such system will interfere any performance of the TEM. If you encounter such problem, is it possible to share your experience? I do know some logger has wifi/bluetooth capability, will this interfere the TEM performances well? whiCurrently we have a temperature logger but its not live and we have to extract the data out from time to time which is very troublesome.

Cheers,
Yee Yan, Tay
FACTS Lab
NTU
Singapore


==============================Original Headers==============================
14, 31 -- From rongchigram79-at-yahoo.com.sg Sat Feb 4 09:48:05 2017
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From marilore504-at-gmail.com Mon Feb 6 18:14:02 2017
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Email: mikeh-at-ncimicro.com Name: mike hehr

Organization: NCI

Title-Subject: [Filtered] EM Sample Preparation Sales Position

Message: Scientific Instrument distributor looking for a Nano Technology
Professional to sell Electron Microscopy sample preparation equipment in
the Midwest. The ideal candidate will have experience in Electron
Micorscopy and knowledge of the various instruments used to perform the
sample prep. These instruments include: Scanning Electron Microscopes,
Ion Millers, Ultra Microtomes, High Pressure Freezers, Critical Point
Dryers, Coaters, Knife Makers and other.

This is a highly consultative sales process that requires extensive
knowledge with EM sample preparation but also the winning spirit to win
the sale and train users on the equipment / techniques for successful
preparation.

Sales Experience preferred, but not needed for the right candidate.

Salary, Commission, Benefits

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From ronaldfaust205-at-gmail.com Wed Feb 8 06:27:11 2017
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Message-ID: {CA32B257.17A4C6A8-at-gmail.com}

Dear Listers,

I am wondering if anyone may have installation/service manual for Jeol
JEM 2100F TEM, or any notes related to its installation you would be
willing to share. I have full operation manual with the instrument, but
need to move it.

Thank you very much beforehand,
--
Valery Ray
www.linkedin.com/in/valeryray
==============================
PBS&T, MEO Engineering Company
290 Broadway, Suite 298
Methuen, MA 01844, USA
Phone: +1-978-305-0479 - leave a message
Mobie: +1-978-305-0479 - leave a message
E-mail: vray-at-partbeamsystech.com
Web: www.partbeamsystech.com
Web: www.freudlabs.com

==============================Original Headers==============================
3, 32 -- From vray-at-partbeamsystech.com Wed Feb 8 11:38:14 2017
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3, 32 -- From: Valery Ray {vray-at-partbeamsystech.com}
3, 32 -- Subject: JEOL JEM 2100F installation/service manual?
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From: microscopy.listserver-at-gmail.com
Date: Wed, 8 Feb 2017 14:13:27 -0600
Subject: [Microscopy] viaWWW:EM Microwave Tissue Processing Workshop February 14th and 15th

Contents Retrieved from Microscopy Listserver Archives
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Email: gavramo-at-fas.harvard.edu Name: Gil Avramovich

Organization: Harvard University Department of Molecular and Cell Biology

Title-Subject: [Filtered] EM Microwave Tissue Processing Workshop

Message: Message: RSVP now for an advanced Microwave Tissue Processing
Workshop for applications in Electron Microscopy, which will be held on
the Cambridge campus of Harvard University, room 254 in NW Labs, 52
Oxford St, Cambridge MA 02138. Join us on February 14th and 15th, and
enjoy complimentary food and coffee, and enjoy learning about cutting
edge technology alongside the foremost experts in Electron Microscopy.
The workshop will cover:
ƒÞ Microwave Technology in Research, ƒÞ Sample Preparation for Microwave
Processing,
ƒÞ Microwave Processing for Electron Microscopy,
ƒÞ Electron Microscopy Polymerization, and
ƒÞ Alternative applications and associated kits. The workshop is totally
free, and spots are limited, so please send your RSVP to
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From: zaluzec-at-microscopy.com
Date: Wed, 8 Feb 2017 14:13:40 -0600
Subject: [Microscopy] viaWWW:Technical Sales Position: Mid Atlantic

Contents Retrieved from Microscopy Listserver Archives
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X-from: rms-at-angstrom.us

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Email: rms-at-angstrom.us Name: Bob Sommerville

Organization: Angstrom Scientific Inc

Title-Subject: [Filtered] Technical Sales Position: Mid Atlantic

Message: Position: Technical Sales Representative - Mid Atlantic US
Territory: Dependent on the candidate but will include MD, DE, DC, VA,
NJ & PA. Products: Sales and support of the following
products/companies: Hitachi: Bench-top Scanning Electron
Microscopes (SEM) and AFM Kleindiek: Nano-manipulators, Probe
Stations and Stages Leica: Electron Microscopy sample prep.
equipment
EMSIS TEM Cameras Deben Tensile Stages and Microscope
AccessoriesJ
V/Bruker Semi: X-ray Diffraction (XRD)


Position Description: This is an exciting position selling and
supporting a range of products in for a number of leading companies in
the electron microscopy and related markets. The ideal candidate will
preferably have a demonstrated track record of success (2-3 years)
selling technical products into the academic, research and industrial
and/or life sciences markets. Optical microscopy or SEM/TEM experience a
definite plus. Academic background should be at min. an associate’s
degree in science (BS or masters preferred). The successful candidate
will live in one of the above listed states. Angstrom Scientific Inc.
is a growing distributor of products targeted to the nanotechnology,
research, life science and semiconductor markets with exciting new and
novel technology. The recent addition of Leica with its impressive
portfolio of EM sample prep. equipment complements and strengthens our
position in this region. Angstrom Scientific Inc. offers competitive
salary and benefits, commission, car allowance and expenses. We are an
equal opportunity employer.
Interested candidates should submit their resume to: Bob
Sommerville, CEO, Angstrom Scientific Inc. e-mail: rms-at-angstrom.us

Keywords: Scanning Electron Microscope, SEM, TEM, EM Sample Preparation,
X-Ray, XRD, Nano-Manipulators, Probing.


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From: microscopy.listserver-at-gmail.com
Date: Thu, 9 Feb 2017 16:24:15 -0600
Subject: [Microscopy] viaWWW:Position open in Basel, Switzerland: Microscopy

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Hello,
It could be that you have different grounding points in use, and an electric potential between them causing a ground current being the issue. You might want to check that your ground lines are correctly wired.

The general recommendation is to use the frame of the TEM as the only grounding point for your TEM connected accessories, arranging a grounding bus at the frame and avoiding any ground loops between the accessories.
For instance, do not use the house grounding line feeding to the power socket which powers the EDX equipment and EDX computer. The house grounding line stays disconnected from this power socket. This socket's grounding (not the house grounding line) becomes linked to the frame of the TEM, instead. If I remember correctly, then Oxford ships an accordingly manipulated distributor with their equipment for easing such wiring. Don't forget to also connect the chassis of the EDX computer to your grounding point at the frame of the TEM.
Consult a certified electrician for assuring electrics security in your setup!

Best greetings from the EM-Labs of CIC biomaGUNE (Spain),
Marco Mller


}
} Email: hexi-at-missouri.edu Name: Xiaoqing He
}
} Organization: Universtiy of Missouri
}
} Title-Subject: [Filtered] Oxford INCA EDS detector dead time 100%
}
} Message: Hi all,
}
} We are having issues with our Oxford EDS detector on our F30. With no
} electron beam on, the dead time stayed at 100% no matter which
} dispersion rate we choose. Initially we thought the misconnection
} between Oxford and TEM may be responsible. But the issue persists even
} after we reboot Microscope PC, Oxford x-stream processor, software.
} etc.
}
} Any inputs are greatly appreciated!
}
} Thanks.
}
} Xiaoqing He


==============================Original Headers==============================
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6, 46 -- Subject: RE: Oxford INCA EDS detector dead time 100%
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From pedugjuf5-at-gmail.com Thu Feb 9 05:44:48 2017
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Email: thomas.horn-at-bsse.ethz.ch Name: Thomas Horn

Organization: ETH Zurich

Title-Subject: [Filtered] Position open in Basel, Switzerland:
Microscopy Engineer/Microscopist

Message: Dear all, we have an open position at the Single Cell Facility
, ETH, Department of Biosystems Science and Engineering (D-BSSE), Basel:
Microscopy Engineer / Microscopist (m/f)
Responsibilities are training and support of facility users. This
includes microscopy instrument quality control and their maintenance.
The microscopy engineer/microscopist will develop and implement
customized configurations and the setup of the experimental workflows of
experiments on the automated imaging systems. The successful candidate
will share responsibilities with our other microscopy expert and work
closely together with our software engineer for image analysis and data
management. This position is available for 2 years and located in Basel.
Requirements are a degree from a technical college or a university with
a proven experience in various advanced light microscopy and digital
imaging technologies. She/he will have extensive technical experience
with state-of-the-art light microscopy platforms such as confocal,
live-cell imaging and/or SPIM. Strong knowledge of advanced quantitative
imaging technologies as well as their underlying optical principles is
required. Scripting experience is a plus. The candidate should have
hands-on skills in microscopy component integration. This position
demands very good communication skills in English to interact with
scientists and students, and the skill to adapt to the service character
of the facility to successfully support scientific research and
operations at the D-BSSE. Besides having excellent technical abilities
in the field of light microscopy, the candidate must be an excellent
team player and possess the ability to work in a variety of scientific
areas. The Single Cell Facility (SCF) is a central scientific core
facility within the ETH Zurich, Department of Biosystems Science and
Engineering (D-BSSE), located in Basel. The SCF serves the department by
providing technology and support in advanced (light-sheet, automated
wide field, confocal) microscopy and flow cytometry.
For further information about the position, please contact Dr. Thomas
Horn, by email: thomas.horn-at-bsse.ethz.ch (no applications).

Applications are only accepted online, please use the “Apply now” button
at the link below. Please address it to: ETH Zurich, Roland Munz, Human
Resources, CH-8092 Zurich.

https://apply.refline.ch/845721/5177/pub/en/index.html

Further information on our facility: https://www.bsse.ethz.ch/scf

Best regards, Thomas Horn.
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From: Colin.Veitch-at-csiro.au
Date: Sun, 12 Feb 2017 20:28:37 -0600
Subject: [Microscopy] Gauss Maus manual

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Organization: Gatan

Title-Subject: [Filtered] EELS Training School - April 2017, California

Message: EELS & EFTEM Analysis Training School

April 4-7, 2017, Gatan R&D Headquarters, Pleasanton , CA

Electron energy loss spectroscopy (EELS) is a powerful technique that
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From shkrsugi29-at-gmail.com Sun Feb 12 11:00:56 2017
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Message-ID: {27532BAF.4AEABBE4-at-gmail.com}

Hi,

We have an old Gauss Maus (Dindima Group/Arlunya) , a hand held device for measuring magnetic fields. We need to work out the magnitude and direction of some stray fields that have just appeared (intermittently of course) in our lab but unfortunately have lost the manual. I have searched on the internet but couldn't find a manual there. If anyone has a copy of the manual they could scan and email to me or, send to me so that I can scan it and send it back it would be much appreciated.

Thank you very much.

Colin Veitch

Manager, Microscopy - Waurn Ponds Laboratory
CSIRO Manufacturing

E colin.veitch-at-csiro.au T +61 3 5246 4891 M 0438 538 475 F +61 3 5246 4057
Address CSIRO Manufacturing, 75 Pigdons Road, Waurn Ponds, Vic 3216, Australia.
www.csiro.au | http://www.csiro.au/Organisation-Structure/Flagships/Manufacturing.aspx


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From: Colin.Veitch-at-csiro.au
Date: Sun, 12 Feb 2017 23:10:22 -0600
Subject: [Microscopy] Gauss Maus manual found.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

With many thanks to Richard Easingwood of Otago University in the "Land of the Long White Cloud" the manual for the Gauss Maus has been found.

Cheers


Colin Veitch

Manager, Microscopy - Waurn Ponds Laboratory
CSIRO Manufacturing

E colin.veitch-at-csiro.au T +61 3 5246 4891 M 0438 538 475 F +61 3 5246 4057
Address CSIRO Manufacturing, 75 Pigdons Road, Waurn Ponds, Vic 3216, Australia.
www.csiro.au | http://www.csiro.au/Organisation-Structure/Flagships/Manufacturing.aspx


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From: bicarbaj-at-mtholyoke.edu
Date: Tue, 14 Feb 2017 10:42:06 -0600
Subject: [Microscopy] TEM: LaB6 cathodes--status?

Contents Retrieved from Microscopy Listserver Archives
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Hello everyone,

Last spring, a thread was started on the quality/longevity of newer
LaB6 cathodes. I looked back through the e-mails and it seemed like
the last thing that was mentioned was something about ongoing
discussions with the vendors.

I have some grant money left that I need to spend soon and was
thinking of switching our CM100 over from W to LaB6. I worry, however,
that it may not be worth it since it seemed like others were having
problems with the longevity of the cathodes.

I did contact the original person who started the thread on this
listserv but have yet to receive a reply. Does anyone know what the
status of the cathodes is?

Thank you,
-Blanca
-----------------------------------------
Blanca Carbajal Gonzalez, M.S.
Director of Microscopy
Science Center
50 College St
Mount Holyoke College
Clapp Laboratory 123
Office: 413-538-3118
Cell: 559-905-7138
bicarbaj-at-mtholyoke.edu
MHC Microscopy

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From: PhillipsT-at-missouri.edu
Date: Fri, 17 Feb 2017 12:05:14 -0600
Subject: [Microscopy] Ultracut S ultramicrotome repair question

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Email: fengxia.liang-at-med.nyu.edu Name: Alice Liang

Organization: New York University Langone Medical Center

Title-Subject: [Filtered] CryoEM manager-NYU

Message: Cryo EM Manager Position at NYU Langone School of Medicine

NYU Langone School of Medicine is seeking a cryo-EM manager to oversee
the operation of our expanding, state-of-the- art electron microscopy
facility. The facility will include Titan Krios and Talos Arctica
microscopes, both with direct detectors, for single particle EM and cryo
electron tomography. The facility will be staffed by 3 team members,
including the manager (this position) and two junior associates. The
facility manager will be responsible for oversight and maintenance of
these microscopes as well as training and assisting users, who will be
primarily from the NYU research community. The manager will interface
with our High Performance Computing team to ensure a seamless interface
between data collection and data processing. The manager will also have
the opportunity to, and will be encouraged to interact with cryo EM
experts in the New York area and to attend key EM conferences (e.g. EM
Gordon Conference) in order to integrate NYU with the greater EM
community, and to implement cutting-edge techniques and technologies
into our facility.

Responsibilities (together with 2 other team members):
Maintain Talos Arctica and Krios microscopes. This duty requires
interfacing with FEI engineers to ensure optimal performance of the
microscopes.
Maintain ancillary, cryo-EM related equipment such as Vitrobot, glow
discharger etc.
Load samples and align microscopes prior to data collection
Train and oversee users for data collection Evaluate quality of data
obtained by users
Interface and collaborate with High Performance Computing team, who will
assist with computational needs for optimal data collection, storage and
data processing
Interface and collaborate with Microscopy Core, which houses electron
microscopes for preliminary screening of samples
Implement data collection strategies that will benefit our user
community Maintain accurate logs for microscope performance and usage

Key element of the job:
This facility will be optimized for high throughput data collection.
Developing a pipeline that fits well with our users will be a key part
of this job. Although the manager is expected to consult with faculty
supervisors, he/she will be given flexibility and responsibility to
develop a pipeline that generates high quality data in an efficient manner.

Requirements:
PhD in structural biology, biochemistry or related field
At least 2 years of cryo EM experience, with expertise in handling
samples, using microscopes and evaluating images
Excellent communication and interpersonal skills
Knowledge of the principles of transmission electron microscopy
General understanding of single particle cryo EM workflow; experience in
cryo electron tomography is a plus Special consideration will be given
to candidates with management experience or a record of achievement in
collaborative, team-driven environments

The Ideal Candidate:
In addition to meeting all the requirements above, the ideal candidate
will also bring enthusiasm and passion to developing our new facility.
We are looking for a colleague who will be excited about interacting
with researchers at all levels and will consistently strive to maintain
a state-of-the-art facility. The EM manager will be an key component of
our new cryo EM initiative, and as such, will play a crucial role in
shaping the new facility. This job is an excellent opportunity for an
electron microscopist who is interested in a leadership role within the
vibrant research community at NYU School of Medicine.

Salary will be competitive and job title will depend on qualifications
of the candidate.

To apply for this position, please email cryoemnyu-at-gmail.com and
include: 1) Cover letter
2) CV
3) Contact information for 3 references

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From stepnoah502-at-gmail.com Wed Feb 15 13:56:35 2017
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Message-ID: {FB6D4791.5BCEEE94-at-gmail.com}

We need to remove the top of our Leica Ultracut S ultramicrotome to check something on the electrical circuit for the overhead light source. I am talking about the top plate that carries the binocular microscope that allows you to view the specimen and cutting action.

It looks like there is a screw at the very back of this plate that can be seen from the underside when the plate is rotated 90 degrees. I am guessing that one removes that screw and then you can used the normal hand driven gear drive to move the carrier forward until it is free of the underlying dovetail. Can anyone confirm this. Additionally, where does one disconnect the electrical to the fluorescent light source? Will I see a plug once I remove this screw and move the carrier forward?

Thanks, Tom

Thomas E. Phillips, Ph.D
Curator's Distinguished Teaching Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400
573-882-4712 (office)
573-882-0123 (fax)
phillipst-at-missouri.edu

http://www.biology.missouri.edu/faculty/phillips.html
http://microscopy.missouri.edu



Thomas E. Phillips, Ph.D
Curator's Distinguished Teaching Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400
573-882-4712 (office)
573-882-0123 (fax)
phillipst-at-missouri.edu

http://www.biology.missouri.edu/faculty/phillips.html
http://microscopy.missouri.edu



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From: microscopy.listserver-at-gmail.com
Date: Sat, 18 Feb 2017 11:42:37 -0600
Subject: [Microscopy] viaWWW:FIB STEM EDX

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Email: roger.ristau-at-uconn.edu Name: Roger Ristau

Organization: University of Connecticut

Title-Subject: [Filtered] FIB STEM EDX

Message: A question for anyone with FIB-STEM-EDX system:
Our FEI Helios 460F1 has single-sample Flip Stage (the 'turret' type Flip Stage with 180 deg
rotation) and STEM detector for imaging TEM liftouts in situ. When attempting to acquire EDX of the
lifout, mounted on the Flip Stage, using EDAX Octane Plus SDD EDX, the spectrum is overwhelmed by
the Si peak from the STEM detector itself. If the STEM detector is retracted, the Si peak is
replaced by a Al peak from the stage area situated below the STEM position.

Obviously the EDX detector is 'seeing' the X-rays generated by the e-beam that passes through the
thin liftout sample. However, this problem did not occur in our older Strata FIB with SiLi detector.
I am presuming it may have something to do with the larger collection angle of the SDD. I have
confirmed with EDAX that the detector is installed correctly,the collimator is in position and the
system is operating normally. (There are no problems doing EDX with the sample in the 'bulk stage'
position.)

My temporary solution is to affix a strip of carbon tape to the 'underside' of the Flip Stage to
absorb the transmitted e-beam, eliminating the spurious Al peak, but this is obviously undesirable
as that makes STEM imaging impossible.

Has anyone else encountered this with a similar system?

Cheers
Roger Ristau
Univ of Connecticut

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From: microscopy.listserver-at-gmail.com
Date: Sat, 18 Feb 2017 11:43:23 -0600
Subject: [Microscopy] rviaWWW:EDS detector shutter issue

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Email: ravi.thakkar369-at-gmail.com Name: Ravi

Title-Subject: [Filtered] EDS detector shutter issue.

Message: Hi Listeners,
I am using Tecnai TEM equiped with Oxford EDS. Whenever I open the shutter to start the EDS
analysis, it's not getting open, it shows overload, while it was not even opened for the days. Could
you guys help me to fix this issue.


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From: microscopy.listserver-at-gmail.com
Date: Sat, 18 Feb 2017 11:51:19 -0600
Subject: [Microscopy] viaWWW:Service Engineer for FEI Tecnai

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Email: steve.sem-at-icloud.com
Name: Steve Perry

Title-Subject: [Filtered] Service Engineer for FEI Tecnai

Message: I would like to hear from a service engineer who is capable and available to perform
service work on our Tecnai F30.

Located on the West coast USA. Travel and accommodations can be provided if required.

Reply in confidence,



Steve Perry


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From: vray-at-partbeamsystech.com
Date: Sat, 18 Feb 2017 13:14:24 -0600
Subject: [Microscopy] Re: viaWWW:FIB STEM EDX

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Hi Roger,

For a quick fix - get Beryllium foil of adequate thickness (0.5mm is
widely available) and cover areas *behind* the retractable STEM detector
exposed to e-beam transmitted through the sample. You could get STEM
image with extended detector and EDS + Se image when detector is retracted.

It is possible that once main Al peak is dealt with, some additional
stray signal generated by electrons scattered from/through the sample
could become detectable - find out where they come from and shield with
Be foil.

Be is considered "hazmat," but that is when it is in
dust/powder/fume/solution form which can be ingested, inhaled, or
absorbed into the body. If you do not drill/sand/machine Be foil but
only attach it by conductive vacuum epoxy it should be safe enough to
stay there. Lots of Be windows were (and still are) out there installed
in SEMs.

If Beryllium is too much of a scare, use Aquadag paint of adequate
thickness to cover area behind STEM detector. Best would be to
manufacture a shield from some kind of metal, coat it with Aquadag,
anneal in vacuum oven -at- 300 degrees, and then mount into SEM chamber.

Best Wishes :)
Valery

Valery Ray
www.linkedin.com/in/valeryray
==============================
PBS&T, MEO Engineering Company
290 Broadway, Suite 298
Methuen, MA 01844, USA
Phone: +1-978-305-0479 - leave a message
Mobie: +1-978-305-0479 - leave a message
E-mail: vray-at-partbeamsystech.com
Web: www.partbeamsystech.com
Web: www.freudlabs.com

On 2/18/2017 12:44 PM, microscopy.listserver-at-gmail.com wrote:
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} Email: roger.ristau-at-uconn.edu Name: Roger Ristau
}
} Organization: University of Connecticut
}
} Title-Subject: [Filtered] FIB STEM EDX
}
} Message: A question for anyone with FIB-STEM-EDX system:
} Our FEI Helios 460F1 has single-sample Flip Stage (the 'turret' type Flip Stage with 180 deg
} rotation) and STEM detector for imaging TEM liftouts in situ. When attempting to acquire EDX of the
} lifout, mounted on the Flip Stage, using EDAX Octane Plus SDD EDX, the spectrum is overwhelmed by
} the Si peak from the STEM detector itself. If the STEM detector is retracted, the Si peak is
} replaced by a Al peak from the stage area situated below the STEM position.
}
} Obviously the EDX detector is 'seeing' the X-rays generated by the e-beam that passes through the
} thin liftout sample. However, this problem did not occur in our older Strata FIB with SiLi detector.
} I am presuming it may have something to do with the larger collection angle of the SDD. I have
} confirmed with EDAX that the detector is installed correctly,the collimator is in position and the
} system is operating normally. (There are no problems doing EDX with the sample in the 'bulk stage'
} position.)
}
} My temporary solution is to affix a strip of carbon tape to the 'underside' of the Flip Stage to
} absorb the transmitted e-beam, eliminating the spurious Al peak, but this is obviously undesirable
} as that makes STEM imaging impossible.
}
} Has anyone else encountered this with a similar system?
}
} Cheers
} Roger Ristau
} Univ of Connecticut
}
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From: dsampson-at-ucsc.edu
Date: Tue, 21 Feb 2017 10:31:38 -0600
Subject: [Microscopy] Free ARL-SEMQ uProbe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,

I have a vintage 1980 ARL-SEMQ microprobe, mostly working, that needs a
home. This was the deluxe model with all of the bells and whistles
available at the time (including CL imaging), and it was updated to the
Advanced Microbeam automation system in the 90's. If you have any
interest please contact me. I need to get it out of the lab soon to make
room for new equipment. I also have nearly an entire second one in
spares. If nobody takes it then it all goes to scrap.

Thanks,

Dan

--
****************************************************************************
A man who lies to himself, and believes his own lies, becomes unable to
recognize truth, either in himself or in anyone else, and he ends up losing
respect for himself and for others. -Fyodor Dostoevsky, novelist (1821-1881)
****************************************************************************
Daniel E. Sampson
Instrument Engineer
A232 Earth and Marine Sciences
University of California
Santa Cruz, CA 95064
dsampson-at-ucsc.edu
(831) 459-4992 office
(831) 359-9075 cell
(831) 459-3074 FAX
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From: microscopy.listserver-at-gmail.com
Date: Tue, 21 Feb 2017 21:06:15 -0600
Subject: [Microscopy] viaWWW:Hitachi SU6600 FE-SEM Constant Beam Drift?

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Email: ibarke2-at-uwo.ca Name: Ivan Barker

Organization: Western University

Title-Subject: [Filtered] Hitachi SU6600 FE-SEM Constant Beam Drift?

Message: Hi all,

A quick question that I hope someone could help me with.

In our daily routine analyses with our Hitachi SU6600 FE-SEM, we are getting a large component of
beam drift. As in, the image seems to migrate while doing an analysis, leading to blurry images and
maps. When the SEM initially turns out, we can correct for this drift with the stigmators, but
eventually, the range for that tops out, and we cannot get crisp images. This occurs with well
grounded samples, while doing routine imaging or EDS analyses. We typically analyze geological thin
sections, but we coat with carbon and ground with either carbon or silver paint, or copper tape. I
originally thought it was due to my grounding, but it used to be fine, and no matter what I do the
images still drift.

However, if I put things into variable pressure mode it is fine.

It has been a while since we've done a "bakeout", so I'm just wondering if it could be something
related to tip alignment, or if there are particles in the column? What can I do to check the
instrument? I just worry there's something going on with the tip. How can I check that?

Thank you in advance for any info and tips!
- Ivan

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From: microscopy.listserver-at-gmail.com
Date: Tue, 21 Feb 2017 21:07:04 -0600
Subject: [Microscopy] viaWWW:3D modeling software

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Email: krueger.eugene-at-mayo.edu Name: Eugene Krueger

Organization: Mayo Clinic, Rochester, MN

Title-Subject: [Filtered] 3D modeling software

Message: Hello microscopists and vendors,
I was wondering what commercial software packages people are using for 3D modeling of confocal
Z-stacks.I can generate beautifully detailed Z-stacks, but would like to be able to make models from
the data that I could use in other applications. Specifically, I would like to generate 3D models
that I could manipulate and use in movies and presentations. Any information would be appreciated,
and vendors are encouraged to reply.

Thank you,
-Eugene

Eugene Krueger
Sr. Research Tech II
Mayo Clinic

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From: vray-at-partbeamsystech.com
Date: Wed, 22 Feb 2017 00:04:35 -0600
Subject: [Microscopy] Re: viaWWW:Hitachi SU6600 FE-SEM Constant Beam Drift?

Contents Retrieved from Microscopy Listserver Archives
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Hi Ivan,

Although it is hard to diagnose remotely, chances are that problem is
not with the gun. If drift was related to electron source, then most
likely it would be appearing similarly in both HV and variable pressure
modes.

There is a good chance that there is some kind of dielectric
contamination, particle, or a piece of fiber stuck at the bottom of
objective lens. It is getting charged during high-vac operation and
deflects/astigmates the beam. When you switch to variable pressure mode
then ADAPT is inserted, shielding contamination from the beam, thus
preventing the drift. You would need to open specimen chamber and
carefully inspect bottom of the column and opening in the polepiece with
mirror and a good flashlight, and try to look inside of the polepiece
opening. Also take a look on outside of the insertable variable aperture
for any traces of contamination or particles. Make sure that in
high-vacuum mode ADAPT is fully retracted. Check resistance from
polepiece to ground with ohmmeter, however strange this sounds. Also
check grounding of your stage - there is some possibility that stage is
electrically floating and thus getting charged in High Vac mode.

Best Wishes,

Valery Ray
www.linkedin.com/in/valeryray
==============================
PBS&T, MEO Engineering Company
290 Broadway, Suite 298
Methuen, MA 01844, USA
Phone: +1-978-305-0479 - leave a message
Mobie: +1-978-305-0479 - leave a message
E-mail: vray-at-partbeamsystech.com
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Web: www.freudlabs.com

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} Email: ibarke2-at-uwo.ca Name: Ivan Barker
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} Organization: Western University
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} Title-Subject: [Filtered] Hitachi SU6600 FE-SEM Constant Beam Drift?
}
} Message: Hi all,
}
} A quick question that I hope someone could help me with.
}
} In our daily routine analyses with our Hitachi SU6600 FE-SEM, we are getting a large component of
} beam drift. As in, the image seems to migrate while doing an analysis, leading to blurry images and
} maps. When the SEM initially turns out, we can correct for this drift with the stigmators, but
} eventually, the range for that tops out, and we cannot get crisp images. This occurs with well
} grounded samples, while doing routine imaging or EDS analyses. We typically analyze geological thin
} sections, but we coat with carbon and ground with either carbon or silver paint, or copper tape. I
} originally thought it was due to my grounding, but it used to be fine, and no matter what I do the
} images still drift.
}
} However, if I put things into variable pressure mode it is fine.
}
} It has been a while since we've done a "bakeout", so I'm just wondering if it could be something
} related to tip alignment, or if there are particles in the column? What can I do to check the
} instrument? I just worry there's something going on with the tip. How can I check that?
}
} Thank you in advance for any info and tips!
} - Ivan
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From: John.Mardinly-at-asu.edu
Date: Fri, 24 Feb 2017 10:21:40 -0600
Subject: [Microscopy] Mildred Dresselhaus died on Monday in Cambridge, Mass. She was 86.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We had a similar problem with a Hitachi S-2460N many years ago. I don't know how it compares to the 6600.

We had let the PM schedule lapse and got similar drifting. The engineer came in and cleaned out the liner tube, as I recall. It had built up a deposit on one side that took on a charge in hivac mode. Once it was cleaned, things were back to normal with no drift in hivac or lovac mode.

Warren Straszheim
Materials Analysis and Research Lab
Iowa State University

-----Original Message-----
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Sent: Wednesday, February 22, 2017 12:06 AM
To: Straszheim, Warren E [BIOTC]

Mildred Dresselhaus, a professor emerita at the Massachusetts Institute of Technology whose research into the fundamental properties of carbon helped transform it into the superstar of modern materials science and the nanotechnology industry, died on Monday in Cambridge, Mass. She was 86.

A. John Mardinly, Ph.D., P.E.
Retired Principal Materials Nanoanalysis Engineer










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From: amit.welcomes.u-at-gmail.com
Date: Sat, 25 Feb 2017 04:39:52 -0600
Subject: [Microscopy] Is this paper available anywhere?

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Rose H (1990) Outline of a spherically corrected semi-aplanatic
medium-voltage TEM. Optik 85: 19–24.

I cannot find it anywhere on internet. Optik journal somehow only show
issues till 112. Is it obtainable from anywhere?


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From: microscopy.listserver-at-gmail.com
Date: Sat, 25 Feb 2017 09:49:55 -0600
Subject: [Microscopy] viaWWW:Carbon Grains on Formvar Grids or Carbon Film

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Email: vakimler-at-oakland.edu Name: Vickie Kimler

Organization: Oakland University Eye Research Institute

Title-Subject: [Filtered] Carbon Grains on Formvar Grids or Carbon Film

Message: Sometimes it is very difficult to tell between what is sample and what is carbon grain
(either grids coated with compressed carbon) or the carbon on Formvar grids. Can anyone shed light
on this when one does protein analysis?

We are working at 100kV and 140Kx mag.

Thanks!

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From: rok210-at-lehigh.edu
Date: Mon, 27 Feb 2017 10:23:13 -0600
Subject: [Microscopy] Re: viaWWW:Carbon Grains on Formvar Grids or Carbon Film

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Hi Vickie,

carbon films come in different grades one of which is 'ultra-thin' and
has areas down to 3nm thick according to the publicity in catalogs.
I've never done protein analysis, but you need a supporting substrate
and one that is amorphous, so try and look in the holes since they may
be covered with thin carbon, the thinner the better.

I have bought some silicon nitride supports that are thin and they can
be plasma cleaned between uses, but they are a bit pricey.

Good luck
Rob

--
Robert Keyse, DPhil
Research Scientist
Lehigh University

5, East Packer Avenue,
Whitaker Laboratory
Bethlehem,
PA 18015-3194

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Fax (610)758-4244


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From: bicarbaj-at-mtholyoke.edu
Date: Mon, 27 Feb 2017 13:49:17 -0600
Subject: [Microscopy] NESM Spring Meeting March 2nd

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Hello everyone,

The New England Society for Microscopy's Spring Meeting this year will
be held at GE Healthcare in Marlborough, MA this week, March 2nd from
5pm-8pm.

For more information and to register, please visit our website:
http://nesmicroscopy.org/upcoming-meetings/

We hope to see you there!

Cheers,
-NESM board

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From: microscopy.listserver-at-gmail.com
Date: Wed, 1 Mar 2017 06:10:39 -0600
Subject: [Microscopy] viaWWW:Ultramicrotomes and gridstainers

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Email: allan.mitchell-at-otago.ac.nz Name: Allan Mitchell

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Title-Subject: [Filtered] Ultramicrotomes and gridstainers

Message: We are in the process of trying to obtain funding to replace one of our ultramicrotomes
(Ultracut E) and our aged LKB Ultrostainer. We are looking at
- RMC Boeckeler ultramicrotme PT-PCZ and the RMC Boeckeler QG-3100 TEM Stainer, or

- Leica Ultracut EM UC7 ultramicrotome and Leica EM AC20 grid-stainer.

I would be keen to hear of peoples experiences with any of these instruments, positives /negatives.

Many thanks

Allan

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From: microscopy.listserver-at-gmail.com
Date: Thu, 2 Mar 2017 06:49:17 -0600
Subject: [Microscopy] viaWWW: Ultramicrotomes and gridstainers

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Email: duraine-at-bcm.edu Name: Lita Duraine

Organization: Howard Hughes Medical Institute

Title-Subject: [Filtered] Re: viaWWW:Ultramicrotomes and gridstainers

Message: Hi Allan,

I totally support the Leica Ultracut EM UC7 microtome. We bought one in 2013 and it has worked
efficiently ever since. It is very user friendly and the program settings are easy to manage.
Hardly any break-in time. The thickness settings stay consistent. You also have a wide latitude
for cutting speeds. There are several light settings especially helpful when I am hand trimming a
block and then want to go immediately to cutting thins. This instrument is so accurate that it can
cut silver sections effortlessly even on the 11th floor of our building! We loved it so much that
we bought a second one two years later when we expanded our lab.
Customer service is another reason why I use Leica. We had a small static shock occurring in the
new room where we put the microtome. Not sure what was causing it, I called Leica. Immediately,
they sent an engineer to our location in Houston and checked everything on the UC7. Several issues
were found within the room actually, and everything turned out great. The UC7 is a workhorse, and
never skips a beat. I highly recommend the Leica UC7 Ultramicrotome.

Lita Duraine
Certified Electron Microscopist
Howard Hughes Medical Institute
Houston, TX 77030


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From: microscopy.listserver-at-gmail.com
Date: Thu, 2 Mar 2017 06:50:14 -0600
Subject: [Microscopy] viaWWW:The Materials Ultramicrotomy Workshop

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Title-Subject: [Filtered] The Materials Ultramicrotomy Workshop

Message: Three days of hands-on training for technicians, researchers, and students who want to
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embedded contamination, and is cheaper than a FIB.

Details:
Wednesday - Friday
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EMS Microscopy Academy
Hatfield, Pennsylvania, USA

Instructors:
Helmut Gnaegi, Diatome Ltd., Switzerland Robert Ranner, Leica, Austria
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From: ph2-at-sprynet.com
Date: Thu, 2 Mar 2017 18:55:26 -0600
Subject: [Microscopy] Inter/Micro 2017, Chicago, IL June 12-16, 2017

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FYI


Inter/Micro 2017
69th Annual International Microscopy Conference
June 12 - 16, 2017
at
McCrone Research Institute, 2820 S. Michigan Avenue, Chicago, IL 60616

Call for Papers: Speaker Presentations

June 12-14: McCrone Research Institute cordially invites you to give a
presentation of your microscopy research at the 69th annual Inter/Micro
conference in Chicago. Join professional and amateur microscopists from
around the world as they present new research on techniques and
instrumentation, environmental and industrial microscopy, and chemical and
forensic microscopy. Speakers receive at $50 registration discount. The
abstract submission deadline is March 17, 2017. View abstract submission
guidelines at:

https://www.mcri.org/v/1199/Call-for-Papers-Abstract-Submission-Guidelines


Tony
..........................
Andrew Anthony "Tony" Havics, CIH, PE
Environmental, Health & Safety, Microscopy, Materials Science & Forensic
Engineering
pH2, LLC
5250 E US Highway 36, Suite 830
Avon, IN 46123
(317) 718-7020 Office
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aahavics-at-pH2LLC.com
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From: jthompso-at-csusb.edu
Date: Thu, 2 Mar 2017 19:06:26 -0600
Subject: [Microscopy] SEM Need help in understanding loss of image generation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Our Hitachi S-2700 SEM will no longer produce an image. We can only see a series of vertical lines of varying intensity with both secondary electron and backscatter detectors. We do have an electron beam - there are changes in intensity of the vertical lines during adjustment of filament current, beam tilt, beam horizon, changes in the lines if we move the specimen. We do lose the image if the HV is turned off on the secondary electron detector. A very similar pattern is seen with the backscatter detector, so it does not appear to be a detector issue.

We have exchanged circuit boards and even the entire column (!) from a second identical scope used for parts. A commercial technician was unable to correct the problem. His best guess is that one or more of the lenses are not functioning.

Any suggestions as to what the problem is? I can send images off-line.


Jeffrey Thompson, Ph.D.
Professor of Biology
California State University
5500 University Pkwy
San Bernardino, CA 92407
909-537-5315



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7, 49 -- Subject: SEM Need help in understanding loss of image generation
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From: vray-at-partbeamsystech.com
Date: Thu, 2 Mar 2017 20:48:40 -0600
Subject: [Microscopy] Re: SEM Need help in understanding loss of image

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Jeffrey,

Please feel free to send or post images to look at, but most likely you
have a simple deflection problem. Remember that in SEM lenses are only
used to form the beam, but magnification (and formation of image) comes
from ratio between area physicall scanned by electron beam on surface of
the sample and area of the CRT screen (or digital image) representing
the image. If you see only vertical lines then chances are that primary
electron beam is not scanned in Y direction over the sample, so in every
line of image you are seeing information from the same exact line
physically scanned by electron beam on the sample. Find a local tech who
knows how to use oscilloscope and able to figure out amplifiers driving
inductive loads (scan coils)....

Valery Ray
www.linkedin.com/in/valeryray
==============================
PBS&T, MEO Engineering Company
290 Broadway, Suite 298
Methuen, MA 01844, USA
Phone: +1-978-296-5063 - leave a message
Mobie: +1-978-305-0479 - leave a message
E-mail: vray-at-partbeamsystech.com
Web: www.partbeamsystech.com
Web: www.freudlabs.com

On 3/2/2017 8:07 PM, jthompso-at-csusb.edu wrote:
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} Our Hitachi S-2700 SEM will no longer produce an image. We can only see a series of vertical lines of varying intensity with both secondary electron and backscatter detectors. We do have an electron beam - there are changes in intensity of the vertical lines during adjustment of filament current, beam tilt, beam horizon, changes in the lines if we move the specimen. We do lose the image if the HV is turned off on the secondary electron detector. A very similar pattern is seen with the backscatter detector, so it does not appear to be a detector issue.
}
} We have exchanged circuit boards and even the entire column (!) from a second identical scope used for parts. A commercial technician was unable to correct the problem. His best guess is that one or more of the lenses are not functioning.
}
} Any suggestions as to what the problem is? I can send images off-line.
}
}
} Jeffrey Thompson, Ph.D.
} Professor of Biology
} California State University
} 5500 University Pkwy
} San Bernardino, CA 92407
} 909-537-5315
}
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} 7, 49 -- Subject: SEM Need help in understanding loss of image generation
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4, 35 -- generation
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From: microscopy.listserver-at-gmail.com
Date: Thu, 2 Mar 2017 23:06:20 -0600
Subject: [Microscopy] viaWWW:Cryo-in-the-Sun Workshop

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From: Nicolas.Stephant-at-univ-nantes.fr
Date: Fri, 3 Mar 2017 02:02:11 -0600
Subject: [Microscopy] Re: SEM Need help in understanding loss of image generation

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Hi Jeffrey,
I'm agree with Valery, a deflection circuit is faulty. A deflection
problem is also possible from the screen circuit. This can be check by
connecting the video output on an external monitor. This microscope has
probably two stages of deflectors to scan the beam and each of them set
with X and Y deflection coils. The most common problem is coming from
power transistor on horizontal circuit. This component is more
sollicited because the scan speed horizontal is faster than the
vertical. This electronic is sensitive to temperature of the room and of
the cooling water. The fastest is the speed the more those transistors
are working, if you can set your microscope to slow scan mode when you
don't use, it's safety for deflection circuit. High magnification
position is also good.

Nicolas STEPHANT

Universit de Nantes
Institut Jean Rouxel
Service de microscopie lectronique balayage et microanalyse
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"Le monde n'existe que pour autant que nous sommes capables d'en produire une image"
C.G Jung

Le 03/03/2017 02:21, jthompso-at-csusb.edu a crit :
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} Our Hitachi S-2700 SEM will no longer produce an image. We can only see a series of vertical lines of varying intensity with both secondary electron and backscatter detectors. We do have an electron beam - there are changes in intensity of the vertical lines during adjustment of filament current, beam tilt, beam horizon, changes in the lines if we move the specimen. We do lose the image if the HV is turned off on the secondary electron detector. A very similar pattern is seen with the backscatter detector, so it does not appear to be a detector issue.
}
} We have exchanged circuit boards and even the entire column (!) from a second identical scope used for parts. A commercial technician was unable to correct the problem. His best guess is that one or more of the lenses are not functioning.
}
} Any suggestions as to what the problem is? I can send images off-line.
}
}
} Jeffrey Thompson, Ph.D.
} Professor of Biology
} California State University
} 5500 University Pkwy
} San Bernardino, CA 92407
} 909-537-5315
}
}
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} 7, 49 -- Subject: SEM Need help in understanding loss of image generation
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7, 26 -- From Nicolas.Stephant-at-univ-nantes.fr Fri Mar 3 02:02:10 2017
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From: protrain-at-emcourses.com
Date: Sat, 4 Mar 2017 03:46:47 -0600
Subject: [Microscopy] SEM: are LaB6 emitters fit for EDS and WDS?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,
a friend of mine is interested in buying a SEM with a LaB6 emitter, for
imaging and, above all, analytical applications: EDS and WDS. However,
he was told that LaB6 emitters are not a good choice for analytical
applications in a SEM, mainly due to stability issues, which - if I got
it correctly - would require a long wait time before reliable spectra
could be acquired.
I have no experience with such a machine, so I can't advice him about
this. Maybe some of you can help me.
Thank you in advance.
Regards

Davide

~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~
Davide Cristofori

direct phone = 041.234.67.26
e-mail = dcristofori[at]unive[dot]it

Centro di Microscopia Elettronica "Giovanni Stevanato" e
Dipartimento di Sc. Molecolari e Nanosistemi
Universit Ca' Foscari Venezia

Campus Scientifico, Edificio ETA
Via Torino 155 I-30172 Mestre (VE) Italy
~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~

==============================Original Headers==============================
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6, 46 -- From: Davide Cristofori {dcristofori-at-unive.it}
6, 46 -- To: microscopy-at-microscopy.com
6, 46 -- Subject: SEM: are LaB6 emitters fit for EDS and WDS?
6, 46 -- Message-ID: {b75d8a9c-5ac7-9147-523f-d53f3c49537f-at-unive.it}
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From medinaluca1-at-gmail.com Fri Mar 3 16:42:07 2017
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Message-ID: {8F6BD8DD.8098AF46-at-gmail.com}

Hi

I know of no problems with LaB6 emitters with regard to stability, provide
that the vacuum level in the electron gun is suitable for their use. Could
you be confusing the instability problem with cold field emitter
instruments, where their natural emission is prone to early and late
instabilities during long operating session.
Regards

Steve

Steve Chapman FRMS
Protrain for Consultancy and Training in Electron Microscopy
+44 (0)7711 606967
web www.emcourses.com



-----Original Message-----
X-from: dcristofori-at-unive.it [mailto:dcristofori-at-unive.it]
Sent: 03 March 2017 16:24
To: protrain-at-emcourses.com

Dear Listers,
a friend of mine is interested in buying a SEM with a LaB6 emitter, for
imaging and, above all, analytical applications: EDS and WDS. However, he
was told that LaB6 emitters are not a good choice for analytical
applications in a SEM, mainly due to stability issues, which - if I got it
correctly - would require a long wait time before reliable spectra could be
acquired.
I have no experience with such a machine, so I can't advice him about this.
Maybe some of you can help me.
Thank you in advance.
Regards

Davide

~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~ Davide Cristofori

direct phone = 041.234.67.26
e-mail = dcristofori[at]unive[dot]it

Centro di Microscopia Elettronica "Giovanni Stevanato" e Dipartimento di Sc.
Molecolari e Nanosistemi Universit Ca' Foscari Venezia

Campus Scientifico, Edificio ETA
Via Torino 155 I-30172 Mestre (VE) Italy ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~
~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~

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microscopy-at-microscopy.com 6, 46 -- Subject: SEM: are LaB6 emitters fit for
EDS and WDS?
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From: microscopy.listserver-at-gmail.com
Date: Sat, 4 Mar 2017 07:35:26 -0600
Subject: [Microscopy] viaWWW:Northern California Society for Microscopy Meeting March 16

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Email: roseann.csencsits-at-schafercorp.com Name: Roseann Csencsits

Organization: Schafer Laboratory

Title-Subject: [Filtered] Northern California Society for Microscopy Meeting March 16

Message: MSA Fellow and Featured speaker: Professor William Landis

"A possible role in vertebrate mineralization for the small, non-collagenous protein, osteocalcin,
as determined by immunocytochemistry"

Lecture includes: standard and high voltage TEM, computer simulation, immunocytochemistry, and
nucleation and growth of Hydroxyapatite

TEM, biology, and materials science - 3 in one! For what more could one ask?

Hosted by EAG, 810 Kifer Road, Sunnyvale, CA
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From: microscopy.listserver-at-gmail.com
Date: Tue, 7 Mar 2017 07:14:33 -0600
Subject: [Microscopy] viaWWW:Do you know about these electron dese bodies in ER?

Contents Retrieved from Microscopy Listserver Archives
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Dear List
I am looking for SEM images of the various types/states of human white blood
cells. Does anybody know any accessible source of such images that is
scientifically sound?
Thanks for your time
yorgos

Dr Yorgos Nikas
Athens Innovative Microscopy
Skra 36 Voula 16673 GREECE

Tel/fax +30 210 8957677
mobile +30 6945 107477
www.eikonika.netwww.aim.cat
*************************************





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5, 20 -- From eikonika-at-otenet.gr Sun Mar 5 01:52:51 2017
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5, 20 -- Subject: leucocyte types in SEM
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From donahenr845-at-gmail.com Mon Mar 6 03:48:07 2017
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Email: kuryu-at-rockefeller.edu Name: Hiro Uryu

Organization: rockefeller university

Title-Subject: [Filtered] Do you know about these electon dese bodies in ER?

Message: Dear list,

I was searching for a significance of electron dense bodies that appeared to be spherical and
located inside of ER. These may be heavily electron dense with or without an electron opaque core.
So far I found one image in the following link, indicting a type of structured that I have seen.
http://classes.kumc.edu/som/CellBiology/organelles/smoother/index.html
Does anyone have an idea what they might be and what the biological significance might be associated
with? I would love to hear your understanding of these dense bodies. I would be happy to share my
image if you would connect with me off site. Many thanks,

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From: nizets2-at-yahoo.com
Date: Tue, 7 Mar 2017 07:20:34 -0600
Subject: [Microscopy] Re: viaWWW:Ultramicrotomes and gridstainers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Allan!

We use a Leica EM UC6 for 12 years now, it is solid as a rock and precise like a swiss clock.
I just had to put one drop of oil in the gears mechanism after 10 years (3 screws to unscrew, I was able to do it myself!), which I accomplished very bravely :-D
Now I am troubled because I don't know anymore if the most loyal companion of men is the dog or a Leica ultramicrotome!

Regards,
Stephane



--------------------------------------------
On Wed, 3/1/17, microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} wrote:

Subject: [Microscopy] viaWWW:Ultramicrotomes and gridstainers
To: nizets2-at-yahoo.com
Date: Wednesday, March 1, 2017, 1:16 PM




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Email: allan.mitchell-at-otago.ac.nz
Name: Allan Mitchell

Organization: Otago centre for Electron Microscopy,
University of Otago

Title-Subject: [Filtered] Ultramicrotomes and gridstainers

Message: We are in the process of trying to obtain funding
to replace one of our ultramicrotomes
(Ultracut E) and our aged LKB Ultrostainer.  We are
looking at
- RMC Boeckeler ultramicrotme PT-PCZ and the RMC Boeckeler
QG-3100 TEM Stainer, or

- Leica Ultracut EM UC7 ultramicrotome and Leica EM AC20
grid-stainer.

I would be keen to hear of peoples experiences with any of
these instruments, positives /negatives.

Many thanks

Allan

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From: ahk-at-bu.edu
Date: Wed, 8 Mar 2017 14:13:50 -0600
Subject: [Microscopy] JEOL 6100 SEM available

Contents Retrieved from Microscopy Listserver Archives
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Hello all:

We are in process of decommissioning our JEOL 6100 ( 25 yo), and it will be removed from lab in two weeks to make room for another equipment. The tool was last used over a year ago in good working condition, and had been under PM contract since new. It has an Oxford EDS (ISIS) unit, IR chamber camera, and specimen current meter on it. Please contact me if you are interested in taking it as a whole, or parts of it. You will need to pay for the packing and shipping of the tool from Boston to your location. Thanks.

Anlee Krupp
Laboratory Manager
Precision Measurement Laboratory
Boston University Photonics Center
8 Saint Mary's Street, Boston, MA 02215
Phone: (617) 353-9044
Email: ahk-at-bu.edu



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From: microscopy.listserver-at-gmail.com
Date: 29th March 2017
Subject: [Microscopy] viaWWW:Free Webinar - Revealing Cellular Dynamics with Millisecond

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Email: lon.nelson-at-leica-microsystems.com Name: LON NELSON

Organization: Leica Microsystems

Title-Subject: [Filtered] Free Webinar - Revealing Cellular Dynamics with Millisecond Precision
*Commercial Posting*

Message: **Commercial Posting**

Leica Microsystems and BitesizeBio have organized a free web-seminar in March that may be of
interest to you:

Revealing Cellular Dynamics with Millisecond Precision – The New Tool That Turned Electron
Micrographs in Motion Picture of Neural Communication


Presenters:
Dr. Shigeki Watanabe
Johns Hopkins School of Medicine

Dr. Frédéric Leroux
Leica Microsystems

What if you can dissect the cellular dynamics with millisecond precision?
What if you can unravel the morphological transformation of a neuron millisecond by millisecond
using electron microscopy?
Could this be even possible?

In this webinar, we will talk about how optogenetics coupled with high-pressure freezing can make
this possible.
We will discuss how to implement electrical stimulation and why it is superior to light stimulation.
We will also discuss the importance of sample processing and the challenges you would face while
freezing different types of samples.

Very best regards,

Lon Nelson
Director of Sales – Microscopy
lon.nelson-at-leica-microsystems.com
M +1 224-628-2467 | F +1 847-607-3160
Leica Microsystems, Inc.
1700 Leider Ln | Buffalo Grove, IL 60089 (USA)


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From: dcristofori-at-unive.it
Date: Fri, 10 Mar 2017 09:05:22 -0600
Subject: [Microscopy] SEM: are LaB6 emitters fit for EDS and WDS? - moving to LaB6 heating

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Dear Listers,
thanks to all of you who answered my question.
LaB6 emitters are unanimously considered stable, yet some of you pointed
out that the filament has to be kept heated in order to assure a stable
emission, even when not working, e.g. overnight.

I'd like to figure out how common is this approach, and the life of LaB6
emitters operated in this way. It would be very interesting and useful
to know your experience also about this point, if you want to share it.
Thanks in advance
Regards

Davide

~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~
Davide Cristofori

direct phone = 041.234.67.26
e-mail = dcristofori[at]unive[dot]it

Centro di Microscopia Elettronica "Giovanni Stevanato" e
Dipartimento di Sc. Molecolari e Nanosistemi
Universit Ca' Foscari Venezia

Campus Scientifico, Edificio ETA
Via Torino 155 I-30172 Mestre (VE) Italy
~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~

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7, 47 -- Subject: SEM: are LaB6 emitters fit for EDS and WDS? - moving to LaB6 heating
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From: FMonson-at-wcupa.edu
Date: Fri, 10 Mar 2017 09:42:03 -0600
Subject: [Microscopy] SEM: are LaB6 emitters fit for EDS and WDS? - moving to LaB6 heating

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Compare FEI 12T and FEI Quanta 400 ESEM, both tungsten emitters (exchangeable).
Quanta 400 ESEM Average life = 60-80 hr
When Quanta vented to change specimen, entire column is vented with AIR (could be better with N2, but not often)
Tecnai 12T (120kV) Average life = 1000 hr**
When change specimen (single tilt) current condition of filament is maintained until vacuum in specimen chamber has recovered.

Quanta filament is OFF overnight.
Tecnai filament is under low current and minimum vacuum pressure 24/7 (10-8 with no break in vacuum in life).

Hope this helps too.

Fred Monson

Frederick C. Monson, PhD
Technical Director
Center for Microanalysis and Imaging, Research and Training (CMIRT)
West Chester University of PA
Geology-Astronomy
750 South Church St.
West Chester, PA, 19383
fmonson-at-wcupa.edu
610-738-0437(Work)


-----Original Message-----
X-from: dcristofori-at-unive.it [mailto:dcristofori-at-unive.it]
Sent: Friday, March 10, 2017 10:18 AM
To: Monson, Frederick {FMonson-at-wcupa.edu}

Dear Listers,
thanks to all of you who answered my question.
LaB6 emitters are unanimously considered stable, yet some of you pointed out that the filament has to be kept heated in order to assure a stable emission, even when not working, e.g. overnight.

I'd like to figure out how common is this approach, and the life of LaB6 emitters operated in this way. It would be very interesting and useful to know your experience also about this point, if you want to share it.
Thanks in advance
Regards

Davide

~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~ Davide Cristofori

direct phone = 041.234.67.26
e-mail = dcristofori[at]unive[dot]it

Centro di Microscopia Elettronica "Giovanni Stevanato" e Dipartimento di Sc. Molecolari e Nanosistemi Universit Ca' Foscari Venezia

Campus Scientifico, Edificio ETA
Via Torino 155 I-30172 Mestre (VE) Italy ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~

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7, 47 -- From: Davide Cristofori {dcristofori-at-unive.it} 7, 47 -- To: microscopy-at-microscopy.com 7, 47 -- Subject: SEM: are LaB6 emitters fit for EDS and WDS? - moving to LaB6 heating 7, 47 -- issue 7, 47 -- Message-ID: {406156fe-7675-14cf-b0d7-306b164a74cc-at-unive.it}
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18, 44 -- Subject: RE: [Microscopy] SEM: are LaB6 emitters fit for EDS and WDS? - moving
18, 44 -- to LaB6 heating
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From: kraftpiano-at-gmail.com
Date: Fri, 17 Mar 2017 10:27:37 -0500
Subject: [Microscopy] Leo 982 software?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

LaB6 emitters, like any other source, outgas when first heated and may then
be unstable. A way of getting round this is to run the filament at a very
low current, just to keep it warm, even if the instrument is not being used.
I do not know if they do it now, but JEOL with LaB6 systems set the filament
heating at half value when you "turned it off".
At very low emission there is no source evaporation, so the filament life
does not suffer.

Steve

Steve Chapman FRMS
Protrain for Consultancy and Training in Electron Microscopy
+44 (0)7711 606967
web www.emcourses.com


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From glenshan72-at-gmail.com Tue Mar 14 16:34:56 2017
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Have not got any messages from the list for a while. Just testing.

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From voraisai2-at-gmail.com Thu Mar 16 13:04:03 2017
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Message-ID: {91004185.C02BA2C8-at-gmail.com}

Does anybody have floppies from an old Leo 982 Gemini column? I just got one in the shop with the hard drive removed, and have to restore the on-board computer. (If you’ve got an old parts Leo 982 and have a spare drive with the software installed, and would be willing to part with it, that would work, too…)

Thank you,

Justin A. Kraft

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From: Gregory.Hendricks-at-umassmed.edu
Date: Sun, 19 Mar 2017 20:28:12 -0500
Subject: [Microscopy] viaWWW:Ultramicrotomes and gridstainers

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Email: pscallio-at-dal.ca Name: Pat Scallion

Organization: Institute for Research in Materials Dalhousie University

Title-Subject: [Filtered] S4700 column hot after a power outage

Message: Hi,
I have seen odd behavior from the Hitachi S4700, after a power outage. When I arrived at work, the
front instrument panel was showing only symbols, not numbers. Also, in addition to other usual
things, the column was very hot, and the metal ticking, like what is heard during a bakeout.

I cannot get any image from the upper, TTL detector, and the beam is showing instability, with
flashes of brightness and image jumping when in use. The Vext is slower to rise as well, and the IP3
starts at 1x10 -8, then rises to 1x10 -7 while the beam is on. The typical vacuum level for IP3 has
been 1x10 -6.

I can send more information if specifics are needed.

Hope someone can help me.

Thanks,
Pat Scallion

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From harremme142-at-gmail.com Sun Mar 19 15:03:37 2017
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Message-ID: {FE6C826B.46F47528-at-gmail.com}

Good Morning Allan,
I just purchased our second Leica UC7. These are great ultramicrotomes; very compact, easy to use and excellent lighting.
I highly recommend them.
Greg

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Email: allan.mitchell-at-otago.ac.nz Name: Allan Mitchell

Organization: Otago centre for Electron Microscopy, University of Otago

Title-Subject: [Filtered] Ultramicrotomes and gridstainers

Message: We are in the process of trying to obtain funding to replace one of our ultramicrotomes (Ultracut E) and our aged LKB Ultrostainer. We are looking at
- RMC Boeckeler ultramicrotme PT-PCZ and the RMC Boeckeler QG-3100 TEM Stainer, or

- Leica Ultracut EM UC7 ultramicrotome and Leica EM AC20 grid-stainer.

I would be keen to hear of peoples experiences with any of these instruments, positives /negatives.

Many thanks

Allan

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From: dcristofori-at-unive.it
Date: Mon, 20 Mar 2017 13:24:24 -0500
Subject: [Microscopy] Re: SEM: are LaB6 emitters fit for EDS and WDS? - moving

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Dear Listers,
here again to sum up the results of my little survey.
It came out that keeping the LaB6 filament heated, even not at a full
range, is quite the standard approach for this emitters.

Many thanks to all the contributors.
Regards

Davide

~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~
Davide Cristofori

direct phone = 041.234.67.26
e-mail = dcristofori[at]unive[dot]it

Centro di Microscopia Elettronica "Giovanni Stevanato" e
Dipartimento di Sc. Molecolari e Nanosistemi
Universit Ca' Foscari Venezia

Campus Scientifico, Edificio ETA
Via Torino 155 I-30172 Mestre (VE) Italy
~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~

Il 10/03/2017 16:14, dcristofori-at-unive.it ha scritto:
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} Dear Listers,
} thanks to all of you who answered my question.
} LaB6 emitters are unanimously considered stable, yet some of you pointed
} out that the filament has to be kept heated in order to assure a stable
} emission, even when not working, e.g. overnight.
}
} I'd like to figure out how common is this approach, and the life of LaB6
} emitters operated in this way. It would be very interesting and useful
} to know your experience also about this point, if you want to share it.
} Thanks in advance
} Regards
}
} Davide
}
} ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~
} Davide Cristofori
}
} direct phone = 041.234.67.26
} e-mail = dcristofori[at]unive[dot]it
}
} Centro di Microscopia Elettronica "Giovanni Stevanato" e
} Dipartimento di Sc. Molecolari e Nanosistemi
} Universit Ca' Foscari Venezia
}
} Campus Scientifico, Edificio ETA
} Via Torino 155 I-30172 Mestre (VE) Italy
} ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~
}
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} 7, 47 -- From: Davide Cristofori {dcristofori-at-unive.it}
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} 7, 47 -- Subject: SEM: are LaB6 emitters fit for EDS and WDS? - moving to LaB6 heating
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8, 49 -- Subject: Re: [Microscopy] SEM: are LaB6 emitters fit for EDS and WDS? - moving
8, 49 -- to LaB6 heating
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From: microscopy.listserver-at-gmail.com
Date: Tue, 21 Mar 2017 14:03:33 -0500
Subject: [Microscopy] viaWWW: Cost of adding an e-beam lithography to a JEOL SEM

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Email: szou-at-american.edu Name: Shouzhong Zou

Organization: American University

Title-Subject: [Filtered] Cost of adding an e-beam lithography to a JEOL SEM

Message: Hi Everyone. We are interested in adding an e-beam lithography to a JEOL JSM-IT100LA SEM.
Do you have any ideas of how much this would cost? We just need a basic EBL to make electrical
contact pads for graphene sheets. Thanks.

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From: microscopy.listserver-at-gmail.com
Date: Thu, 23 Mar 2017 17:08:21 -0500
Subject: [Microscopy] viaWWW:Immuno-gold labeled cellular structures on the membrane?

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Email: zhuoli-at-coh.org Name: Zhuo Li

Organization: City of Hope Beckman Research Institute

Title-Subject: [Filtered] Immuno-gold labeled cellular structures on the
membrane?

Message: Dear Listers,

We did immunogold labeling on MDA-MB-231 breast cancer cells grown on
cover glass to see if the antigen would appear on the cell surface. The
cells were fixed in 4% paraformaldehyde and 0.1% glutaraldehyde in 0.1M
cacodylate buffer pH7.4. Immunogold labeling was performed before
critical point drying and sputter-coating with Au/Pd. Images were taken
on a Zeiss Sigma FE-SEM. Besides some scattered signals, a lot of gold
particles are localized in one area. An example is here:
https://goo.gl/photos/YPXZrwzNAukfsHBw7
Could you tell what the structure it is? Also, there were a lot of gold
particles on the background/cover glass? Could you suggest ways of
eliminating them.

Thank you in advance!

Zhuo Li

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From: leunissen-at-aurion.nl
Date: Thu, 23 Mar 2017 18:07:31 -0500
Subject: [Microscopy] Re: viaWWW:Immuno-gold labeled cellular structures on the membrane?

Contents Retrieved from Microscopy Listserver Archives
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Good morning,

This is not easy to answer in just a few words.

To evaluate the reliability of any immunolabelling it is required to at least check the negative controls: specimens incubated without primary antibody. Based on those results one can then look at background and what to do about it.
If the negative control is clean, you are dealing with labelling based on binding of the primary. Pre-adsorption of the primary can help provide answers as to whether what you observe is specific or non-specific.
If the negative control is not clean, the labelling is the result of interaction between gold conjugate and specimen. In that case incubation protocols and specimen conditioning are the first thing to look at.

Bakground, false positives can almost always be controlled. I will be happy to help with detailed suggestions if you would like, but since that might go into products and brands it would be better to do this off-list.

Cheers from sunny New Zealand,

Jan Leunissen
Aurion

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From: ZZhang-at-uwyo.edu
Date: Thu, 23 Mar 2017 21:19:10 -0500
Subject: [Microscopy] viaWWW:Immuno-gold labeled cellular structures on the membrane?

Contents Retrieved from Microscopy Listserver Archives
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Dear Zhuo Li:

1. It seems, at least, two possibilities for your picture. One is the structure (with gold particles) could be extracellular matrix, which is usually 'sticky' for gold particles, or could contain the antigen. Another possibility is that it is a broken membrane - you are viewing the inner membrane, which might have more antigen, or being sticky. You may want to do a 'control' with full fixation (Glutaraldehyde + Osmium), which provide you a better resolution of the structure.

2. Aldehyde group, especially that from glutaraldehyde is very 'sticky' to antibodies. Since you are NOT viewing the internal structure, I strongly recommend to avoid using glutaraldehyde, not even 0.1%. It may explain, at least in part, the heavy labeling on your cover glass.

3. Blocking with BSA, and/or serum could reduce the non-specific background.

4. I recommend the sample be coated with carbon, instead of Au/Pd. You can then confirm the gold particles with a backscatter electron detector (here is a reference I published many years ago - Localization of myosin on sperm-cell-associated membranes of tobacco (Nicotiana tabacum L.) - https://link.springer.com/article/10.1007/BF01279082

5. As Jan suggested, control is critical!

Hope this helps and let me know if you need a PDF copy of the reference.

Good Luck,

Zhaojie

Zhaojie Zhang, Ph.D.
Director, Jenkins Microscopy Facility
University of Wyoming


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Email: zhuoli-at-coh.org Name: Zhuo Li

Organization: City of Hope Beckman Research Institute

Title-Subject: [Filtered] Immuno-gold labeled cellular structures on the membrane?

Message: Dear Listers,

We did immunogold labeling on MDA-MB-231 breast cancer cells grown on cover glass to see if the antigen would appear on the cell surface. The cells were fixed in 4% paraformaldehyde and 0.1% glutaraldehyde in 0.1M cacodylate buffer pH7.4. Immunogold labeling was performed before critical point drying and sputter-coating with Au/Pd. Images were taken on a Zeiss Sigma FE-SEM. Besides some scattered signals, a lot of gold particles are localized in one area. An example is here:
https://goo.gl/photos/YPXZrwzNAukfsHBw7
Could you tell what the structure it is? Also, there were a lot of gold particles on the background/cover glass? Could you suggest ways of eliminating them.

Thank you in advance!

Zhuo Li

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From: l.kepinski-at-int.pan.wroc.pl
Date: Fri, 24 Mar 2017 14:22:50 -0500
Subject: [Microscopy] Open position SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,
The Institute of Low Temperature and Structure Research, Polish Academy
of Sciences, Wroclaw, Poland has an immediate opening for an Electron
Microscopy Technician.

The main responsibilities and duties are: operation and maintenance of
FE-SEM (equipped with EDS and EBSD) and ancillary equipment; training
and support for users in microscope operation and sample preparation and
participation in scientific projects in the field of materials science.

Job requirements: Graduate (B.Sc is a minimum) in physics, material
science, chemistry, or similar discipline; experience in operation of
SEM and X-ray microanalysis (EDS) (knowledge of EBSD and TEM techniques
will be an advantage); good written and oral skills in English.

To view full job posting please visit :
http://www.intibs.pl/en/the-institute/news/687

Regards,

Leszek Kepinski

Institute of Low Temperature and Structure Research,

Polish Academy of Sciences,

P.O. Box 1410, 50-950 Wroclaw, Poland

e-mail: L.Kepinski-at-int.pan.wroc.pl

==============================Original Headers==============================
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10, 18 -- From: "l.kepinski" {l.kepinski-at-int.pan.wroc.pl}
10, 18 -- To: Microscopy-at-microscopy.com
10, 18 -- Subject: Open position SEM
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From: xinhuolin-at-gmail.com
Date: Fri, 24 Mar 2017 22:27:48 -0500
Subject: [Microscopy] CryoEM/TEM Postdoc Position at Brookhaven National Laboratory

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The postdoctoral associate is expected to develop and perform TEM
characterization of assembly of designed bio-molecular arrays. The
research will involve the development of new approaches for cryoEM,
cryoET, cryoSTEM, liquidEM characterization of an integration of
proteins, peptides and DNA into targeted structures with multiscale
organization, and analysis of their structure and assembly processes.
The work will be conducted in the Electron Microscopy group in the
Center for Functional Nanomaterials which houses five state-of-the-art
scanning/ transmission electron microscopes. The majority of the work
will be conducted on a FEI Environmental Titan equipped with a K2
direct-electron detector, a FEI Talos F200X, and an
aberration-corrected dedicated STEM, first two of which have
cryo-transfer, liquid-flow, low-dose, and automated data acquisition
capabilities. The successful implementation of the work requires a
close interaction with scientists working on bio-inspired
self-assembly and advanced characterization methods using x-ray
scattering.

Review of applications begins immediately. Applications will be
accepted until the position is filled. Research will be under the
direction of two well-known electron microscopists at the CFN in close
collaboration with PIs and postdocs from the Soft/Bio Group, the
Biology Department, and NSLSII.

Required Knowledge, Skills and Abilities:

1. Ph.D. in Physics, Materials Science, Chemistry or Biology
2. Solid background in Transmission Electron Microscopy with rigorous
Ph.D. training in a cryoEM or TEM group.
3. Skills in cryoEM/TEM structural characterization at the atomic scale.

Preferred Knowledge, Skills, and Abilities:

Cryo-electron microscopy, single-particle and tomography methods,
hands-on experience with TEM sample preparation, such as cryo-plunging
and ultramicrotomy

Other Information:

BNL policy states that Research Associate appointments may be made to
those who have received their doctoral degrees within the past five
years.

The EM facility in the CFN includes 5 transmission electron
microscopes, 1 dual-beam FIB, and a collection of specialized holders
including liquid and gas flow holders, liquid electrochemical holders,
heating holders (single-tilt, double-tilt, and high tilt),
cryo-transfer holders (single tilt, double tilt, and high tilt), and
nanoindentation holders (single and double tilt).

Interested candidates can apply online by clicking on the link below:

https://jobs.bnl.gov/job/upton/postdoctoral-research-associate-cryo-em-tem/3437/4240079


Best Regards,
Huolin Xin, Ph.D.
Associate Scientist
Center for Functional Nanomaterials at Brookhaven National Laboratory
and
Adjunct Assistant Professor
Department of Materials Science and Engineering
SUNY Stony Brook University
https://sites.google.com/site/xinhuolin/
Email: hxin-at-bnl.gov
Office: 631-344-4350

==============================Original Headers==============================
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13, 37 -- Subject: CryoEM/TEM Postdoc Position at Brookhaven National Laboratory
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From: xinhuolin-at-gmail.com
Date: Fri, 24 Mar 2017 22:32:21 -0500
Subject: [Microscopy] STEM/Tomography Postdoc Position at Brookhaven National Laboratory

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear TEMers,

I am looking for a postdoc in the area of STEM and electron
tomography. The postdoc will have the opportunity to work with a suite
of tools in the Center for Functional Nanomaterials at Brookhaven
National Laboratory, including an aberration-corrected FEI
Environmental Titan equipped with a K2 direct-electron detector, a FEI
Talos F200X equipped with X-FEG, Super-X, and an Enfinium, an
aberration-corrected cold-FEG dedicated STEM, and a Nion HERMES
(located in Rutgers), first two of which have analytical tomography,
cryoET, cryoEM, and liquidEM capabilities. In total, the facility
includes 5 transmission electron microscopes, 1 dual-beam FIB, and a
collection of specialized holders including liquid and gas flow
holders, liquid electrochemical holders, heating holders (single,
double, and high tilt), cryogenic holders (single tilt, double tilt,
and high tilt), and nanoindentation holders (single and double tilt).

Interested applicants should send a CV, and a one-paragraph
description of his/her research/education background. Review of
applications begins immediately. Applications will be accepted until
the position is filled.

Best Regards,
Huolin Xin, Ph.D.
Associate Scientist
Center for Functional Nanomaterials at Brookhaven National Laboratory
and
Adjunct Assistant Professor
Department of Materials Science and Engineering
SUNY Stony Brook University
https://sites.google.com/site/xinhuolin/
Email: xinhuolin-at-gmail.com
Office: 631-344-4350

==============================Original Headers==============================
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From: microscopy.listserver-at-gmail.com
Date: Mon, 27 Mar 2017 11:44:26 -0500
Subject: [Microscopy] viaWWW:Immuno-gold labeled cellular structures on the membrane?

Contents Retrieved from Microscopy Listserver Archives
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This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
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Email: zhuoli-at-coh.org Name: Zhuo Li

Organization: City of Hope Beckman Research Institute

Title-Subject: [Filtered] Immuno-gold labeled cellular structures on the membrane?

Message: Dear Listers,

We did immunogold labeling on MDA-MB-231 breast cancer cells grown on cover glass to see if the
antigen would appear on the cell surface. The cells were fixed in 4% paraformaldehyde and 0.1%
glutaraldehyde in 0.1M cacodylate buffer pH7.4. Immunogold labeling was performed before critical
point drying and sputter-coating with Au/Pd. Images were taken on a Zeiss Sigma FE-SEM. Besides some
scattered signals, a lot of gold particles are localized in one area. An example is here:
https://goo.gl/photos/YPXZrwzNAukfsHBw7
Could you tell what the structure it is? Also, there were a lot of gold particles on the
background/cover glass? Could you suggest ways of eliminating them.

Thank you in advance!

Zhuo Li

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From: microscopy.listserver-at-gmail.com
Date: Mon, 27 Mar 2017 11:45:11 -0500
Subject: [Microscopy] viaWWW: The Current Level in ET Detectors After the PRE-AMP stage

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Email: anirudha-at-sciencetomorrow.biz Name: Anirudha Borse

Organization: ScienceTomorrow LLC

Title-Subject: [Filtered] The Current Level in ET Detectors After the PRE-AMP stage

Message: Hi,

I have recently started studying SEMs and was looking into the Detector Current value after the
Pre-Amplifier stage and before converting it to Digital Voltage.

Can anyone please help me with that

Thank You
Anirudha
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From: microscopy.listserver-at-gmail.com
Date: Mon, 27 Mar 2017 21:51:16 -0500
Subject: [Microscopy] viaWWW:EM Technical Director position at University of Chicago

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Email: jotham-at-uchicago.edu Name: Joe Austin

Organization: The University of Chicago

Title-Subject: [Filtered] EM Technical Director position at University of Chicago

Message: Dear Colleagues,

I would like to call your attention to a Technical Director position in the Advanced Electron
Microscopy facility at The University of Chicago (Requisition Number 102322).

For more information about the position and to apply please visit this link:
https://jobopportunities.uchicago.edu/applicants/jsp/shared/position/JobDetails_css.jsp?postingId=670183

This position will be open until filled and will be reviewing applications on an ongoing basis.
If you have questions about the position please email Joe Austin at jotham-at-uchicago.edu.
If you have any difficulty uploading your application or any questions, please email Manuel
Carrasquillo at mcarrasquillo-at-bsd.uchicago.edu


The University of Chicago is an Affirmative Action/Equal Opportunity/Disabled/Veterans Employer and
does not discriminate on the basis of race, color, religion, sex, sexual orientation, gender
identity, national or ethnic origin, age, status as an individual with a disability, protected
veteran status, genetic information, or other protected classes under the law. For additional
information please see the University's Notice of Nondiscrimination.
Staff Job seekers in need of a reasonable accommodation to complete the application process should
call 773-834-1841 or email talentacquisition-at-uchicago.edu with their request.

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From: microscopy.listserver-at-gmail.com
Date: Tue, 28 Mar 2017 19:01:35 -0500
Subject: [Microscopy] viaWWW:Cathodoluminescence in SEM

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Email: jpshield-at-uga.edu Name: John P Shields

Organization: University of Georgia

Title-Subject: [Filtered] Cathodoluminescence in SEM

Message: We are in the process of adding CL to our FE-SEM. I am not familiar enough with the
detectors to know if this is a pain or a positive.

Some questions would be: What is the average lifespan (if there is one) for these detectors? What
kind of trouble can you get into with them? Are there any conflicts with other detectors (real or
imagined)? How easy is it to use one?
Any information that cannot be easily googled would be appreciated.

John S

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From: microscopy.listserver-at-gmail.com
Date: Wed, 29 Mar 2017 07:56:07 -0500
Subject: [Microscopy] viaWWW:multiple grid cryo holder

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Email: j.janssen-at-nki.nl Name: Hans Janssen

Organization: The Netherlands Cancer Institute

Title-Subject: [Filtered] multiple grid cryo holder

Message: Does anybody out there have any experience with the Gatan multiple(3)grid cryo holder? We
are specifically interested in the grid locking system.

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From: microscopy.listserver-at-gmail.com
Date: Tue, 4 Apr 2017 06:59:20 -0500
Subject: [Microscopy] viaWWW:Haskris Chiller R075

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Email: Hasan.Ali-at-angstrom.uu.se Name: Hasan Ali

Organization: Uppsala University

Title-Subject: [Filtered] Free Lens Control On JEOL2000FX

Message: Hi,

We have assembled a Gatan energy filter GIF2002 under an old JEOL2000FX. As the GIF doesn't have any
communication with the microscope, we don't have a magnification reduction in the microscope when
using the GIF and the image/diffraction pattern on the viewing screen is magnified about 19X on the
GIF CCD. I want to do angular resolved EELS, but even at the lowest automated camera length (100mm)
in the microscope, I cannot get the two beams simultaneously on the CCD for my sample (the
collection angle is too low). So I need to go to lower camera lengths using "Free Lens Control"
available in the microscpe. I tried Free lens control and obtained the lower camera lengths by
changing the strength of intermediate lenses, but when I switch back to image mode, the settings
there are changes too and the sample is no more on eucentric height. I don't understand exactly now
which lens should I tune in combination with intermediate lenses.
Does anyone have the experience of operating the microscope in Free lens control who could suggest
me something to follow? Also if someone have the manual to operate the JEOL2000FX in Free Lens
Control, that will be very helpful for me. I didn't get information about this in the manuals which
I have.
Thanks

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From fritschemichael755-at-gmail.com Fri Mar 31 04:20:40 2017
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Email: kmoore-at-vetcor.com Name: Kasandra Moore

Organization: Geist Station Animal Hospital

Title-Subject: [Filtered] Maintenance Microscope
Message: We have a Swift Instrument international SA M3200 microscope.
The images are dirty.
We think the microscope needs to be cleaned.
Can someone provide this service in the Indianapolis area?

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From westelle638-at-gmail.com Sun Apr 2 14:48:15 2017
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Message-ID: {AB55A17E.C16BAE9B-at-gmail.com}

The 53rd anniversary meeting of the Southeastern Microscopy Society will be at the Holiday Inn in Athens, GA, on May 24-26, 2017! There is an exciting program planned; Mike Marko of the Wadsworth Center and past president of MSA will be our Invited Speaker. In addition, there will be contributed talks from the members and the Ruska Award student presentations. (Encourage your students to compete in the Ruska Award competition!) A Vendor Social will be held on Wednesday evening, a Banquet on Thursday evening, and a Business Breakfast on Friday morning.

Information is available at http://southeasternmicroscopy.org/2017-2/

Half-day Workshops on Wednesday will include:

RMC and an Array ultramicrotome (at Holiday Inn)
FEI and FE-SEM STEM and analysis (at GEM on UGA campus)
Negative staining with Sara Miller and Mary Ard (at GEM on UGA campus)
Protochips with wet/dry TEM sample holder. (at GEM on UGA campus)
Possible Confocal workshop with Zeiss (at the BioImaging Center at UGA)

The abstract deadline is April 14. Please encourage your students to apply for the Ruska Award! http://southeasternmicroscopy.org/ruska-award-student-competition/

The SEMS website is ready for your registration, available at http://southeasternmicroscopy.org/2017-2/ . You can also get to the hotel reservation webpage by clicking on the link on the meeting website.

We hope to see you in Athens!

Terri Bruce (terri-at-clemson.edu), Program Chair
John Shields (johnshields-at-gmail.com) and Mary Ard (maryard-at-uga.edu), Local Arrangements Committee



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3, 32 -- Subject: Southeastern Microscopy Meeting, May 24-26
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Email: andre.dufresne-at-umanitoba.ca Name: Andr Dufresne

Organization: University of Manitoba Canada

Title-Subject: [Filtered] Haskris Chiller R075

Message: Hy everyone,
I'm looking for a water chiller for a TEM and SEM (H-7000)
The unit I'm looking for is a Haskris R075 or equivalent.
Options:
Water cooled

Message me directly if you have any questions or know the availability of such a unit.
Merci /Thank you.

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From: microscopy.listserver-at-gmail.com
Date: Tue, 4 Apr 2017 07:00:16 -0500
Subject: [Microscopy] viaWWW:Aurion Immuno Gold Silver Staining Workshop

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Email: stacie-at-ems-secure.com Name: Stacie Kirsch

Organization: Electron Microscopy Sciences

Title-Subject: [Filtered] The Aurion Immuno Gold Silver Staining Workshop

Message: Three days of hands-on training for students, researchers, and microscopists who want to
learn the most up to date theory and practice in Immuno Gold labeling.
Details:
Wednesday - Friday
May 9-12, 2017
8:00 a.m. - 4:30 p.m.
EMS Microscopy Academy
Hatfield, Pennsylvania, USA

Instructors:
Peter Van De Plas, Aurion, The Netherlands
Michael Kostrna, Director, EMS Microscopy Academy
Al Coritz, Technical Director, Electron Microscopy Sciences

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From: zaluzec-at-aaem.amc.anl.gov
Date: Tue, 11 Apr 2017 07:15:49 -0500
Subject: [Microscopy] In Memoriam: B. Kestel MSA 1994 Technologist of the Year

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Email: gtorraca-at-amgen.com Name: Gianni Torraca

Organization: MSA

Title-Subject: [Filtered] Freeze fracture TEM of biologics wanted - service work

Message: Good Day,

I am looking for a lab that can perform freeze fracture analysis of a biologic solution.
Kinds regards
Gianni Torraca
Sr. Scientist
Amgen Inc.

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From kimgarr459-at-gmail.com Fri Apr 7 13:02:06 2017
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Message-ID: {A014D037.891BAFC7-at-gmail.com}

Bernard J. "Bernie” Kestel the 1994 Microscopy Society of America’s Technologist of the year passed away
on April 7.

Bernie was an engineering specialist microscopy technologist for 44 years at Argonne National Laboratory, retiring in 2002. Bernie published numerous articles in microscopy journals and was the recipient of the 1994 Technologist of the Year by the Microscopy Society of America, 1996 Pacesetter Award by Argonne National Laboratory and 1998 Outstanding Service Award by the University of Chicago.

His contributions to the community were in the area of specimen preparation, where he developed new techniques, preparated countless specimens and assisted/trained innumerable students, scientists and colleagues. A compendium of his methodology has been published as a scientific report and is freely available to anyone interested in electropolishing of materials.

Kestel B, (1986) Polishing Methods for Metallic and Ceramic Transmission Electron Microscopy Specimens, ANL-80-120/Rev.1 Report , available from NTIS DE89016686 Issue Number 199005 https://ntrl.ntis.gov/NTRL/dashboard/searchResults.xhtml?searchQuery=ANL-80-120

He was an artist in his field and could always be counted on to find a way to make that critical TEM sample, even when you only had 1small item to work with. He will be remembered here at Argonne and by those he helped in the community, myself included.


http://www.friedrichjones.com/obits/obituaries.php/obitID/985197?utm_source=Argonne+Today&utm_campaign=fd54afd940-ATS_2017_04_11&utm_medium=email&utm_term=0_91cdd2aa04-fd54afd940-237434973

===========================================
Dr. Nestor J. Zaluzec
Argonne National Laboratory
Photon Sciences Division
9700 S. Cass Ave
Bldg 212 / A-143
Argonne, Illinois 60439 USA
Email: Zaluzec-at-aaem.amc.anl.gov


Tel: 530-NES-TORZ (530-637-8679) has Voice Mail
Lab: 630-252-7901
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iChat: Zaluzec-at-AIM.com
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Senior Scientist - Argonne National Laboratory
Fellow of the Microscopy Society of America
Senior Fellow the Computational Institute - University of Chicago
E.P. Wigner Fellow - Oak Ridge National Laboratory
Past President Microscopy Society of America
Adjunct Professor of Physics - Northern Illinois University &
the University of Illinois at Chicago
Visiting Professor of Microscopy - Manchester University

===========================================
TPMLab: http://tpm.amc.anl.gov
MMSite: http://www.amc.anl.gov
===========================================

The box said ...
"This program requires Win 95/98/NT or better..."
So I bought a Mac !

===========================================
LLAP
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From: cannonmp-at-comcast.net
Date: Thu, 13 Apr 2017 02:19:50 -0500
Subject: [Microscopy] Pinouts for Oxford Pentafet Pre-Amp

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***********************************************************************************
Forwarded from "Ask a Microscopist"
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not a member the listserver, and
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Name: Richard Gursky
School: St. Jude Children’s Research Hospital
Grade/Education Level: Graduate
Location: Memphis, TN
US
Email: richard.gursky-at-stjude.org

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Listers,

This is mostly for those of you on the list who work as applications specialists or service engineers for microscope companies. Perhaps you can send some good advice to Brakel about how to get such a job.
Please remember to respond directly to Brakel -- kbrakel91-at-gmail.com

I've already sent some ideas to the Brakel, but since I do not work as an applications person or a service engineer, some pointers from people who do would be more useful.

---------------
Philip Oshel
Microscopy Society of America
Ask a Microscopist
www(dot)microscopy(dot)org/resources/ask(dot)cfm

Name: Kiralyn Brakel
School: Texas A&M University
Grade/Education Level: Graduate
Location: College Station, TX
US
Email: kbrakel91-at-gmail.com

I'm trying to find the pre-amp pinout designations on an Oxford 7215 Link
Pentafet EDS spectrometer. Oxford no longer supports this once highly
touted piece of gear and have been unable to help me. Can anyone help with
that?

Keeping legacy gear running is made much more difficult by lack of support.
I have operated a small electron microprobe lab here in Seattle since 1984
and have useful gear from the entire history of x-ray microanalysis. I'm
now using an old ARL SEMQ and a JEOL JXA 8600. I once had the ARL EMX-SM
that had the actual moon rocks in it. My computers go all the way back to
DEC PDP11s, though that's long gone. The SEMQ still uses MS 6.2. I have
thought it might be good to have a used gear forum of some sort. Facebook
page ? Wordpress blog ? Any thoughts on that too.

Bart Cannon / Cannon Microprobe


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From: microscopy.listserver-at-gmail.com
Date: Fri, 14 Apr 2017 06:38:42 -0500
Subject: [Microscopy] viaWWW:celsian or barium feldspar specimens

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Email: Linda.Davis-at-Vesuvius.com Name: Linda L Davis

Organization: Vesuvius USA

Title-Subject: [Filtered] celsian or barium feldspar specimens

Message: Hello All,

We have a new chemist in our R&D Analytical Group who needs to do some development work on analyzing
barite and celsian using an ICP. I've found nice barite crystals to purchase, but now I need some
celsian/barium feldspar. I cannot use eBay at all for work, so I have come to the listserve to see
if anyone has any celsian we can purchase or have. I don't need a ton, but numerous crystals/grains
to grind up for digestion and analysis.
I would greatly appreciate any help.

Sincerely,

Linda L. Davis

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From: microscopy.listserver-at-gmail.com
Date: Fri, 14 Apr 2017 06:39:32 -0500
Subject: [Microscopy] viaWWW: Whole Cell Mount TEM - Carbon Coating

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Email: vakimler-at-oakland.edu Name: Vickie Kimler

Organization: Oakland University Eye Research Institute

Title-Subject: [Filtered] Whole Cell Mount TEM - Carbon Coating

Message: When one does whole cell mount TEM nowadays on gold-Formvar grids, is it necessary to
carbon-coat the samples after preparation (seeding, fixation, staining, dehydration and critical
point drying by CO2) or is before the preparation sufficient?

Grids with cells will be stored in a dessicator filled with Drier-Rite.

Thanks,
Vickie

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From: microscopy.listserver-at-gmail.com
Date: Sun, 16 Apr 2017 08:58:39 -0500
Subject: [Microscopy] viaWWW:Electron Microscopy Technologist Position Open

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Name: Richard Gursky
School: St. Jude Children’s Research Hospital
Grade/Education Level: Graduate
Location: Memphis, TN
US
Email: richard.gursky-at-stjude.org

Hello Shouzhong,

I would contact Joe Nabity to see if his Nanometer Pattern Generation
System can be added to your 'scope. We use it on our FIB and are happy
with the performance. You can find more information here:
http://www.jcnabity.com/

I have no ties, financial or otherwise, to Joe or his company.

Thanks,
Chris

On Tue, Mar 21, 2017 at 3:15 PM, {microscopy.listserver-at-gmail.com} wrote:
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} Title-Subject: [Filtered] Cost of adding an e-beam lithography to a JEOL SEM
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} Message: Hi Everyone. We are interested in adding an e-beam lithography to a JEOL JSM-IT100LA SEM.
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Email: mryan-at-cshl.edu Name: Marjorie Ryan

Organization: Cold Spring Harbor Laboratory

Title-Subject: [Filtered] Searching for an Electron Microscopy Technologist

Message: Cold Spring Harbor Laboratory seeks a highly motivated dedicated individual to work in a
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From: microscopy.listserver-at-gmail.com
Date: Mon, 17 Apr 2017 21:18:48 -0500
Subject: [Microscopy] viaWWW:Source of Standard zinc oxide and iron oxide sample for EDS

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On Apr 14, 2017, at 7:39 AM, microscopy.listserver-at-gmail.com
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} Title-Subject: [Filtered] celsian or barium feldspar specimens
}
} Message: Hello All,
}
} We have a new chemist in our R&D Analytical Group who needs to do some development work on analyzing
} barite and celsian using an ICP. I've found nice barite crystals to purchase, but now I need some
} celsian/barium feldspar. I cannot use eBay at all for work, so I have come to the listserve to see
} if anyone has any celsian we can purchase or have. I don't need a ton, but numerous crystals/grains
} to grind up for digestion and analysis.
} I would greatly appreciate any help.
}
} Sincerely,
}
} Linda L. Davis
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From wandjoan59-at-gmail.com Mon Apr 17 04:05:20 2017
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Email: ravi.thakkar369-at-gmail.com Name: Ravi

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Title-Subject: [Filtered] Source of Standard zinc oxide and iron oxide sample for EDS reference.

Message: Hi All,
One of the user for our EM facility is looking for Zinc Oxide and Iron Oxide as standard reference
for EDS analysis. If any one can help. Thanks in advance.
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From: wesaia-at-iastate.edu
Date: Tue, 18 Apr 2017 05:55:43 -0500
Subject: [Microscopy] viaWWW:Source of Standard zinc oxide and iron oxide sample for EDS

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We may need more information before we can answer the question well - at least, I would need more.

Is this EDS in conjunction with TEM or SEM? The form of the material would different for the two applications.
- If TEM, I don't have much to suggest since I do SEM. It seems they would want a thin film or powder.
- If SEM, they would probably want a homogenous, bulk sample. They can get bulk samples of iron oxide. Does it matter if it is Fe2O3 or Fe3O4? I am used to zinc oxide being a powder. It might be challenging to find it in bulk form.

What do they ultimately want to know? Do they want to know if their material matches? Do they want to quantify the oxide? Do they want to see if they have excess oxygen?
I often find users coming in with too narrow a question. When I found out the true issue, there is usually much more freedom in suggesting a solution.

Why do they want the oxides? Most EDS systems will have standards built-in for the elements. They are often quite good. I wouldn't think that they need the oxides of the metals.

What form is their sample in, bulk, powder, film? If it is not flat, polished, thick material, then accurate quant will be out of the question.

Hopefully the answer is not simply "Because". I find it much easier to help someone who is forthcoming with information rather than one who is dead-set on a single course of action.

Regards,
Warren Straszheim

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Title-Subject: [Filtered] Source of Standard zinc oxide and iron oxide sample for EDS reference.

Message: Hi All,
One of the user for our EM facility is looking for Zinc Oxide and Iron Oxide as standard reference for EDS analysis. If any one can help. Thanks in advance.
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25, 57 -- Subject: RE: [Microscopy] viaWWW:Source of Standard zinc oxide and iron oxide
25, 57 -- sample for EDS
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From: kleopullin-at-email.arizona.edu
Date: Tue, 18 Apr 2017 18:31:44 -0500
Subject: [Microscopy] HRTEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I'm struggling with Williams and Carter (2nd edition, I think)
understanding HRTEM. I generally find their text approachable and easy
to read, but not the HRTEM material, other than the math.

Is their a text or article that is more detailed? Outside of the
Supposedly HRTEM sources that I have found have lengthy introductions
to the basic, non-HR microscope, then brief descriptions of the math
of HRTEM.

What's a good Read?

I'm a microscopist, with a B.S., so technical is okay, but I want a
deep focus on HRTEM, theory and instrumentation.

Thanks!

Kleo (Kathleen) Pullin
Moraga, CA
209-610-0555
kleopullin-at-email.arizona.edu
https://www.linkedin.com/in/kleopullin
https://twitter.com/resolvingdust

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From: microscopy.listserver-at-gmail.com
Date: Tue, 18 Apr 2017 19:31:21 -0500
Subject: [Microscopy] Re: viaWWW:Source of Standard zinc oxide and iron oxide

Contents Retrieved from Microscopy Listserver Archives
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Hi Ravi,

There are some sources for standards mentioned in this thread:

http://probesoftware.com/smf/index.php?topic=889.msg5674#msg5674

john


On 4/17/2017 7:28 PM, microscopy.listserver-at-gmail.com wrote:
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} Title-Subject: [Filtered] Source of Standard zinc oxide and iron oxide sample for EDS reference.
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10, 51 -- Subject: Re: [Microscopy] viaWWW:Source of Standard zinc oxide and iron oxide
10, 51 -- sample for EDS
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From: microscopy.listserver-at-gmail.com
Date: Tue, 18 Apr 2017 19:31:51 -0500
Subject: [Microscopy] viaWWW:Source of Standard zinc oxide and iron

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I am not affiliated with Ted Pella, but they do sell EDS/WDS mineral/oxide standards.

http://www.tedpella.com/calibration_html/UHV-EL_Reference_Standards_for_EDS_WDS.htm



On Tue, Apr 18, 2017 at 7:17 AM, {wesaia-at-iastate.edu {mailto:wesaia-at-iastate.edu} } wrote:




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We may need more information before we can answer the question well - at least, I would need more.

Is this EDS in conjunction with TEM or SEM? The form of the material would different for the two
applications.
- If TEM, I don't have much to suggest since I do SEM. It seems they would want a thin film or
powder.
- If SEM, they would probably want a homogenous, bulk sample. They can get bulk samples of iron
oxide. Does it matter if it is Fe2O3 or Fe3O4? I am used to zinc oxide being a powder. It might
be challenging to find it in bulk form.

What do they ultimately want to know? Do they want to know if their material matches? Do they
want to quantify the oxide? Do they want to see if they have excess oxygen?
I often find users coming in with too narrow a question. When I found out the true issue, there
is usually much more freedom in suggesting a solution.

Why do they want the oxides? Most EDS systems will have standards built-in for the elements.
They are often quite good. I wouldn't think that they need the oxides of the metals.

What form is their sample in, bulk, powder, film? If it is not flat, polished, thick material,
then accurate quant will be out of the question.

Hopefully the answer is not simply "Because". I find it much easier to help someone who is
forthcoming with information rather than one who is dead-set on a single course of action.

Regards,
Warren Straszheim

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Subject: [Microscopy] viaWWW:Source of Standard zinc oxide and iron oxide sample for EDS




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Title-Subject: [Filtered] Source of Standard zinc oxide and iron oxide sample for EDS reference.

Message: Hi All,
One of the user for our EM facility is looking for Zinc Oxide and Iron Oxide as standard
reference for EDS analysis. If any one can help. Thanks in advance.
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25, 57 -- From: "Straszheim, Warren E [BIOTC]" {wesaia-at-iastate.edu {mailto:wesaia-at-iastate.edu} }
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25, 57 -- Subject: RE: [Microscopy] viaWWW:Source of Standard zinc oxide and iron oxide
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From: microscopy.listserver-at-gmail.com
Date: Tue, 18 Apr 2017 19:46:09 -0500
Subject: [Microscopy] viaWWW:need 700-800 nm excitation for Zeiss AxioObserver

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Email: michael.cammer-at-med.nyu.edu Name: Michael Cammer

Organization: NYULMC

Title-Subject: [Filtered] need 700-800 nm excitation for Zeiss AxioObserver

Message: We need to excite in the 700 to 800 nm range and the Zeiss HPX-120 lamp we have is filtered
to block wavelengths above 660 nm. Rather than customize the filter inside the lamp house, we are
looking for an alternative light source for the 700-800 nm range with a liquid light guide or fiber
that we could swap for the Zeiss HPX-120 on the days we need to image infra-red.

Are there any simple light sources that fit this description that cost less than $3k? If not, how
about ones that don’t cost much more than this?

Thank you!


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From: nmz787-at-gmail.com
Date: Tue, 18 Apr 2017 20:51:45 -0500
Subject: [Microscopy] Re: viaWWW:need 700-800 nm excitation for Zeiss AxioObserver

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I'd say it depends on how stable you want the line (frequency) to be.

CD-ROM lasers are 780nm, and super cheap thanks to economies of scale.
Getting one coupled to the fiber of your choice would then be your
challenge.

On Tue, Apr 18, 2017 at 6:08 PM, {microscopy.listserver-at-gmail.com} wrote:
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} Email: michael.cammer-at-med.nyu.edu Name: Michael Cammer
}
} Organization: NYULMC
}
} Title-Subject: [Filtered] need 700-800 nm excitation for Zeiss AxioObserver
}
} Message: We need to excite in the 700 to 800 nm range and the Zeiss HPX-120 lamp we have is filtered
} to block wavelengths above 660 nm. Rather than customize the filter inside the lamp house, we are
} looking for an alternative light source for the 700-800 nm range with a liquid light guide or fiber
} that we could swap for the Zeiss HPX-120 on the days we need to image infra-red.
}
} Are there any simple light sources that fit this description that cost less than $3k? If not, how
} about ones that don’t cost much more than this?
}
} Thank you!


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From: benada-at-biomed.cas.cz
Date: Wed, 19 Apr 2017 09:52:13 -0500
Subject: [Microscopy] Re: FW: Ask a Microscopist- converting EDS files in

Contents Retrieved from Microscopy Listserver Archives
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***********************************************************************************
Forwarded from "Ask a Microscopist"
Please remember that the person asking the question is likely
not a member the listserver, and
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-----Original Message-----
X-from: "AssociationManagement-at-microscopy.org" {associationmanagement-at-microscopy.org}

Hello Anuja,
Please look at the WEB page of NIST DTSA II software.

{http://www.cstl.nist.gov/div837/837.02/epq/dtsa2/}

It should be possible to import .spx file into this software and then export the data in cvs format.
Unfortunately I do not have any spx file at hand, but I have tested it on msa and spc files and it works well.

Best regards from Prague

Oldrich

--
Oldřich Benada
Institute of Microbiology CAS, v.v.i.
Laboratory of Molecular Structure Characterization
Vídeňská 1083
142 20 Prague 4
Czech Republic

On Wed, 19 Apr 2017 06:59:11 -0500, oshel1pe-at-cmich.edu wrote :
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} -----Original Message-----
} X-from: "AssociationManagement-at-microscopy.org"
} {associationmanagement-at-microscopy.org} Date: Wednesday, 19April,
} 2017 at 01:08 To: "AssociationManagement-at-microscopy.org"
} {associationmanagement-at-microscopy.org} , Philip Oshel
} {oshel1pe-at-cmich.edu} Subject: Ask a Microscopist
}
} Name:Anuja Bhalkikar
} School:University of Nebraska Lincoln
} Grade/Education Level:Graduate
} Location:Lincoln, NE
} Email:anuja.bhalkikar-at-huskers.unl.edu
} Subject:Convert .spx data to .txt or .csvYour
} Question:Hello, I recently used an FEI Tecnai Osiris S/TEM to obtain
} EDS of my sample. I believe we have a Super-X EDX detection system in
} conjunction with Bruker Esprit software. The data was unfortunately
} saved as .spx. Is there any way I could convert it into .txt or .csv
} format? Thanks, Anuja
}
}
}
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From: jkrupp-at-deltacollege.edu
Date: Wed, 19 Apr 2017 15:29:05 -0500
Subject: [Microscopy] Update on Spurr's Plastic Please

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings

I’m trying to round out my understanding of different embedding media and need the latest opinions on Spurr’s LVEM.

I have used it in the past, it was OK, and never worried about it.

Now I see that some workers avoid it due to more toxic ingredients, more difficulty staining, and problems with brittle blocks. Also, like Epon 812 before, I see that some of the original components have been replaced with newer substitutes.

I teach students how to embed tissue and want to be able to tell them the latest opinions from other experts in case I am missing something.

Thanks

Jon

Jonathan Krupp
ASB&T
San Joaquin Delta College
5151Pacific Ave.
Stockton, CA 95207
(209) 954-5284
jkrupp-at-deltacollege.edu



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From: peter.eschbach-at-comcast.net
Date: Thu, 20 Apr 2017 00:02:50 -0500
Subject: [Microscopy] Re: HRTEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Kleo

Check out the Oxford text: high Resolution Electron Microscopy by Spence.

A close second and less Graduate level math is found in "Transmission Electron Microscopy and Diffractory Materials" Springer by Fultz and Howe.

You are looking for a discussion of "Pendellosung". Page 631 Fultz and Howe. To explain it to Students at U of O that I taught, I built a coupled pendulum, where the one pendulum represents the non diffracted beam and the other s Bragg reflected beam. The coupling represents the lattice. The pendulum will stop and start and that time can represent thickness of da sample. And so white spots in HRTEM , nodes, could be channels between atoms or atoms depending on how thick the sample is ( how much time pendulum swings )


Pete Eschbach
Oregon State University

Sent from my iPhone

} On Apr 18, 2017, at 4:47 PM, kleopullin-at-email.arizona.edu wrote:
}
}
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} I'm struggling with Williams and Carter (2nd edition, I think)
} understanding HRTEM. I generally find their text approachable and easy
} to read, but not the HRTEM material, other than the math.
}
} Is their a text or article that is more detailed? Outside of the
} Supposedly HRTEM sources that I have found have lengthy introductions
} to the basic, non-HR microscope, then brief descriptions of the math
} of HRTEM.
}
} What's a good Read?
}
} I'm a microscopist, with a B.S., so technical is okay, but I want a
} deep focus on HRTEM, theory and instrumentation.
}
} Thanks!
}
} Kleo (Kathleen) Pullin
} Moraga, CA
} 209-610-0555
} kleopullin-at-email.arizona.edu
} https://www.linkedin.com/in/kleopullin
} https://twitter.com/resolvingdust
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From: Joseph.Mowery-at-ARS.USDA.GOV
Date: Thu, 20 Apr 2017 09:41:54 -0500
Subject: [Microscopy] Update on Spurr's Plastic Please

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Good Morning,

After trying Epon, I’ve never felt the need to use Spurr's again. For some reason the Spurr’s recipe proportions vary between some vendors. I’ve been really happy with the LX-112 (Epon) from LaddResearch, their mixing instruction are right on point, and I can store the 2 components in syringes in the fridge for about 6 months and just mix them with the accelerator prior to use. Epon seem to section better than Spurr's, and is more stable under the beam. I've also heard good things about the Epon-Araldite mixture.

-Joe

Joe Mowery | Biologist
Electron and Confocal Microscopy Unit
USDA, Agricultural Research Service
Beltsville, MD



-----Original Message-----
X-from: jkrupp-at-deltacollege.edu [mailto:jkrupp-at-deltacollege.edu]
Sent: Wednesday, April 19, 2017 4:49 PM
To: Mowery, Joseph

Greetings

I’m trying to round out my understanding of different embedding media and need the latest opinions on Spurr’s LVEM.

I have used it in the past, it was OK, and never worried about it.

Now I see that some workers avoid it due to more toxic ingredients, more difficulty staining, and problems with brittle blocks. Also, like Epon 812 before, I see that some of the original components have been replaced with newer substitutes.

I teach students how to embed tissue and want to be able to tell them the latest opinions from other experts in case I am missing something.

Thanks

Jon

Jonathan Krupp
ASB&T
San Joaquin Delta College
5151Pacific Ave.
Stockton, CA 95207
(209) 954-5284
jkrupp-at-deltacollege.edu



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27, 123 -- From: "Mowery, Joseph" {Joseph.Mowery-at-ARS.USDA.GOV}
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From: bozhilov-at-ucr.edu
Date: Tue, 25 Apr 2017 13:03:18 -0500
Subject: [Microscopy] XL30-FEG SEM

Contents Retrieved from Microscopy Listserver Archives
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Dear All,
We are interested in anyone having an old Gatan GIF 200, or a more recent EEL spectrometer for a Tecnai 30, who needs to have it taken off their hands.
Thanks,

Ken

Johns Hopkins University

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From watkinsmelanie58-at-gmail.com Mon Apr 24 19:52:57 2017
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Message-ID: {7000395D.B9311C48-at-gmail.com}

Here at UC Riverside we have a Philips XL30-FEG SEM that we are planning to remove from service.
If anyone is interested in acquiring this system please contact us.

The SEM is in perfect fully operational condition.
It was installed in 1996 and it has been under continuous parts and labor maintenance contract with the FEI Co. since.
Here is the configuration of the system:

FEG Schottky field emitter
Continuously adjustable accelerating voltage from 200 V to 30 kV
Resolution
2.0 nm at 30 kV
5.0 nm at 1 kV
Detectors
Everhard-Thornley SE/BSE (ETD)
Solid-state BSED
Manually-controilled specimen stage.
IGP and diffusion pump vacuum system
specimen chamber with 52-pin electrical feedthrough for electrical measurements
the original Windows 3.11 PC has been ungraded to Windows 2000 PC.
Digital image acquisition as well as Polaroid camera.

Analytical System
EDAX Inc. Phoenix/Genesis EDX system
liquid N2 cooled Si(Li) EDX detector 10mm2
Resolution Mn Kα - 129 eV
SATW window for detection of elements from Beryllium up
EBSD system - Hamamatsu video camera and HKL Channel5 software package for EBSD data acquisition and analysis

Krassimir Bozhilov

phone 951 312 8296
fax 951 827 2489
bozhilov-at-ucr.edu

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From: microscopy.listserver-at-gmail.com
Date: Tue, 25 Apr 2017 22:34:03 -0500
Subject: [Microscopy] viaWWW:Aurion and Bio SEM Workshops

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From: jkrupp-at-deltacollege.edu
Date: Wed, 26 Apr 2017 15:23:14 -0500
Subject: [Microscopy] Formalin

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I have a bottle of Formalin that has a white precipitate, probably polymerized formaldehyde from being stored in the refrigerator.

Is there anything I can do to return the solution to normal?

Thanks

Jon

Jonathan Krupp
ASB&T
San Joaquin Delta College
5151Pacific Ave.
Stockton, CA 95207
(209) 954-5284
jkrupp-at-deltacollege.edu



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From: barnesa-at-umn.edu
Date: Wed, 26 Apr 2017 15:49:33 -0500
Subject: [Microscopy] Re: Formalin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Technically you can heat the solution to try and depolymerize the
presumptive paraformaldehyde oligomers. I do *not* recommend trying
this - it works occasionally, but hot formalin is almost never a good
idea even in a hood.

Option 1: Send it to Haz Waste and buy another bottle.
Option 2: Filter it and use it anyway.

I've needed to use option 2 a few times over the years - mostly due to
reagent shipping issues and/or "surprise!" samples. In my hands,
filtered PF works okay for straight histology-level work, so-so (at
best) for immuno work, and leads to a range of....interesting results
for EM samples.

I'd still recommend option 1 - formalin is { $10/liter from Fisher. In
a pinch, filtering for H&E or other optical microscopy will probably
be okay.

Aaron


-----
Aaron Barnes, MD, PhD
Clinical Pathology Resident (PGY1) | Dept of Lab Medicine & Pathology
University of Minnesota Medical School
Dunny Lab | Dept of Microbiology & Immunology


On Wed, Apr 26, 2017 at 3:33 PM, {jkrupp-at-deltacollege.edu} wrote:
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} I have a bottle of Formalin that has a white precipitate, probably polymerized formaldehyde from being stored in the refrigerator.
}
} Is there anything I can do to return the solution to normal?
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} Thanks
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} Jon
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From: wij.muss-at-aon.at
Date: Thu, 27 Apr 2017 06:14:53 -0500
Subject: [Microscopy] Re: Formalin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Jonathan (not knowing whether you got additional personal replies which
were not sent via the public listserver):

it might depend:

Is that bottle of Formalin 'labelled$B!F(B ( e. g. adhesive label indicating the
producing company like MERCK, Fisher or whatever company$B!D(B, content, cat.no,
etc.etc. ) and has high percentage of CH2O or is it only a lower
concentrated, "home-made$B!H(B formalin solution made from concentrated
Formaldehyde solution? Has it been prepared with buffer or without buffer (=
hydrous only) solution?

If the former: usually if there has been included a 'stabilisator$B!F(B
(besides 10% MetOH usually calcium carbonate these days, in former days:
"dolomite powder$B!H(B) the white precipitate originates possibly from the
stabilizer;

If the latter: it might be part of stabilizer (if the solution had not
been filtered when it was mixed up) OR it might be in fact deteriorating
FA-oligo- polymers (as Aaron Barnes pointed out) or even precipitated
phosphate or other ions (if the FA-solution was prepared with a buffer). In
that case:

If it is only 1 bottle (1 L or eventually also 1 gallon) I would do the
following:

Either filter or take out most of the solution by a pipet without disturbing
the precipitate and use only for "normal$B!H(B Histology (as Aaron Barnes
pointed out already). The problem arises here only that you don't know about
the real concentration of the fixative.

Or: dispose of (according to your legal & national safety concepts) after
"neutralizing$B!H(B (decomposing) the solution with e.g. concentrated NaOH
(which decomposes FA by means of the CANIZARRO-reaction to produce formic
acid (HCOOH) and methanol (CH3OH) . Since the blocking of free aldehyde
groups (in fixation of tissues for ICH or even ultrastructural
ICH/IC-chemistry ) is done with glycine, sodium-borohydride or even
ammoniumchloride-solutions (or addition of these substances to FA-solution)
you could try to deactivate the FA-solution prior to (+/- safe) disposal by
converting F-aldehyde to alcohol(s). Also addition of bisulfite has been
reported to be efficient.
If you would like to look for other possibilities to dispose of old (perhaps
deteriorated and of uncertain origin ) FA-solutions you might search by
Googleing for: | formaldehyde OR formalin AND deactiv* AND disposal |
(also perhaps read my blog on FA-safe disposal on: https://www.researchgate.
net/post/How_do_you_neutralize_formaldehyde )

Another possibility:
Aldehydes
Many aldehydes are respiratory irritants, and some, such as formaldehyde and
acrolein, are quite toxic. There is sometimes merit in oxidation of
aldehydes to the corresponding carboxylic acids, which are usually less
toxic and less volatile. Procedure for Permanganate Oxidation of 0.1 mol of
Aldehyde 3RCHO + 2KMnO4 $B"*(B 2RCO2K + RCO2H + 2MnO2 + H2O A mixture of 100 mL
of water and 0.1 mol of aldehyde is stirred in a 1-L round-bottomed flask
equipped with a thermometer, dropping funnel, stirrer, steam bath, and, if
the aldehyde boils below 100 $B!k(BC, a condenser. Approximately 30 mL of a
solution of 12.6 g (0.08 mol, 20% excess) of potassium permanganate in 250
mL of water is added over a period of 10 minutes. If the temperature rises
above 45 $B!k(BC, the solution should be cooled. If this addition is not
accompanied by a rise in temperature and loss of the purple permanganate
color, the mixture is heated by the steam bath until a temperature is
reached at which the color is discharged. The rest of the permanganate
solution is added slowly at within 10 $B!k(BC of this temperature. The
temperature is then raised to 70 to 80 $B!k(BC, and stirring continued for 1
hour or until the purple color has disappeared, whichever occurs first. The
mixture is cooled to room temperature and acidified with 6 N sulfuric acid.
(CAUTION: Do not add concentrated sulfuric acid to permanganate solution
because explosive manganese oxide (Mn2O7) may precipitate.) Enough solid
sodium hydrogen sulfite (at least 8.3 g, 0.08 mol) is added with stirring at
20 to 40 $B!k(BC to reduce all the manganese, as indicated by loss of purple
color and dissolution of the solid manganese dioxide. The mixture is washed
down the drain with a large volume of water. If the aldehyde contains a
carbon-carbon double bond, as in the case of the highly toxic acrolein, 4
mol (20% excess) of permanganate per mol of aldehyde is required to oxidize
the alkene bond and the aldehyde group.

PROCEDURES FOR THE LABORATORY-SCALE TREATMENT OF SURPLUS AND WASTE CHEMICALS
(from an article launched to web by University of Geneva and really
helpful):
Formaldehyde is oxidized conveniently to formic acid and carbon dioxide by
sodium hypochlorite. Thus 10 mL of formalin (37% formaldehyde) in 100 mL of
water is stirred into 250 mL of hypochlorite laundry bleach (5.25% NaOC1) at
room temperature and allowed to stand for 20 minutes before being flushed
down the drain. This procedure is not recommended for other aliphatic
aldehydes because it leads to chloro acids, which are more toxic and less
biodegradable than corresponding unchlorinated acids.
https://www.unige.ch/sciences/chiorg/matile/12-ProceduresforLabTreatmentofWa
steChemicals.pdf


Let us know how your problem was solved$B!D(B. Thank you, best regards,
Wolfgang, Salzburg-Austria


============================================================================
===============

Von: barnesa-at-umn.edu [mailto:barnesa-at-umn.edu]
Gesendet: Mittwoch, 26. April 2017 23:10
An: wij.muss-at-aon.at
Betreff: [Microscopy] Re: Formalin This post has been answered too to the
Listserver

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Technically you can heat the solution to try and depolymerize the
presumptive paraformaldehyde oligomers. I do *not* recommend trying this -
it works occasionally, but hot formalin is almost never a good idea even in
a hood.

Option 1: Send it to Haz Waste and buy another bottle.
Option 2: Filter it and use it anyway.

I've needed to use option 2 a few times over the years - mostly due to
reagent shipping issues and/or "surprise!" samples.
In my hands, filtered PF works okay for straight histology-level work, so-so
(at best) for immuno work, and leads to a range of....interesting results
for EM samples.

I'd still recommend option 1 - formalin is { $10/liter from Fisher. In a
pinch, filtering for H&E or other optical microscopy will probably be okay.

Aaron


-----
Aaron Barnes, MD, PhD
Clinical Pathology Resident (PGY1) | Dept of Lab Medicine & Pathology
University of Minnesota Medical School Dunny Lab | Dept of Microbiology &
Immunology

On Wed, Apr 26, 2017 at 3:33 PM, {jkrupp-at-deltacollege.edu} wrote:
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From: microscopy.listserver-at-gmail.com
Date: Thu, 27 Apr 2017 06:53:15 -0500
Subject: [Microscopy] viaWWW: Bruker EDS on Tecnai TEM

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Email: hexi-at-missouri.edu Name: Xiaoqing He

Organization: Electron microscopy core at University of Missouri

Title-Subject: [Filtered] Bruker EDS on Tecnai TEM

Message: Dear Listener,

I am wondering anyone on the list has a Bruker TEM acceptance checklist that you are kindly to
share. Any feedback on the performance of Bruker EDS on FEI Tecnai TEM would be greatly appreciated!

Please send me email privately.

Thanks.
Xiaoqing He

http://emc.missouri.edu/

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From: stefan.diller-at-t-online.de
Date: Fri, 28 Apr 2017 03:23:22 -0500
Subject: [Microscopy] Certified thickness of ultramicrotome cuts - advice needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,

a customer of mine has an ISO certification of the EM lab coming.

One of the problems is to reliably demonstrate the thickness of the 50nm UM slices and the semi-thick 500 nm slices in clinical
workflow.

Normally this is done in the field by using the interference color of the slices in the water tray which is not very accurate and
depends also on the color spectra of the illumination system, the resin system, the accuracy of the ultramicrotome, the user, room
situation; what shall I say more...?

Did anyone of you had this problem and how did you solve it?

This question is also posed to the manufacturers of ultramicrotomes, RMC and LEICA. How do you handle this?

Did anyone measure the thickness of the slices and how? Through-focus-series in TEM? Some setup in SEM?


Best wishes,

Stefan


--


-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.nanoflight.info
www.stefan-diller.com
www.electronmicroscopy.info
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www.zwillingsprojekt.de
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Anfahrt: http://Mail.map24.com/Stefan.Diller
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16, 22 -- From: Stefan Diller {stefan.diller-at-t-online.de}
16, 22 -- Subject: Certified thickness of ultramicrotome cuts - advice needed
16, 22 -- Message-ID: {6140f078-b27e-6584-1786-33d9c9d26bae-at-t-online.de}
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From: lorenzo.menzel-at-fiu.edu
Date: Fri, 28 Apr 2017 10:04:24 -0500
Subject: [Microscopy] Re: Certified thickness of ultramicrotome cuts - advice needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Stefan,

Another thing to consider, is do you care about the thickness of the section (on water, grid, slide), or the thickness of the material removed from the block; i.e. the block advance (assuming the ultramicrotome is cutting all the material off after each advance).

For our studies, the block advance, which can be related back to the original volume of tissue, is what we like to know. If you minimise compression ( ultrasonic diamond knifes, different angles of knife and/or harder resin), then the block advance and the section thickness on grid begin to converge.

Best regards,

Ben


--
Research Support Manager (Imaging and IT Systems)
MRC Brain Network Dynamics Unit,
University of Oxford Department of Pharmacology,
Mansfield Road, Oxford, OX1 3TH, United Kingdom.
Telephone: 01865 271897 {http://www.mrcbndu.ox.ac.uk/}




-----Original Message-----
X-from: stefan.diller-at-t-online.de [mailto:stefan.diller-at-t-online.de]
Sent: 28 April 2017 09:33
To: Ben Micklem {ben.micklem-at-pharm.ox.ac.uk}

Stefan,

I thought the original method to determine section thickness
accurately was to shadow sections, at a known angle with the sections
on mica (or similar), and then calculate the height (thickness) of the
seciton by the length of the shadowed metal deposit and the known
angle. Admittedly, this is a lot of prep work and not something one
would want to introduce into a "routine procedure".

Please correct me, gently, if I am wrong ;-)


Lorenzo

On 4/28/17, stefan.diller-at-t-online.de {stefan.diller-at-t-online.de} wrote:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
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} ----------------------------------------------------------------------------
}
} Dear All,
}
} a customer of mine has an ISO certification of the EM lab coming.
}
} One of the problems is to reliably demonstrate the thickness of the 50nm UM
} slices and the semi-thick 500 nm slices in clinical
} workflow.
}
} Normally this is done in the field by using the interference color of the
} slices in the water tray which is not very accurate and
} depends also on the color spectra of the illumination system, the resin
} system, the accuracy of the ultramicrotome, the user, room
} situation; what shall I say more...?
}
} Did anyone of you had this problem and how did you solve it?
}
} This question is also posed to the manufacturers of ultramicrotomes, RMC and
} LEICA. How do you handle this?
}
} Did anyone measure the thickness of the slices and how? Through-focus-series
} in TEM? Some setup in SEM?
}
}
} Best wishes,
}
} Stefan
}
}
} --
}
}
} -----------------------------------------------------
} Stefan Diller - Scientific Photography
} Arndtstrasse 22
} D - 97072 Wuerzburg Germany
} ++49-931-7848700 Phone
} ++49-931-7848701 Fax
} ++49-175-7177051 Mobile
}
} Websites:
} www.nanoflight.info
} www.stefan-diller.com
} www.electronmicroscopy.info
} www.elektronenmikroskopie.info
} www.zwillingsprojekt.de
} www.assisi.de
} Anfahrt: http://Mail.map24.com/Stefan.Diller
} -----------------------------------------------------
}
}
} ==============================Original
} Headers==============================
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} 16, 22 -- From: Stefan Diller {stefan.diller-at-t-online.de}
} 16, 22 -- Subject: Certified thickness of ultramicrotome cuts - advice
} needed
} 16, 22 -- Message-ID: {6140f078-b27e-6584-1786-33d9c9d26bae-at-t-online.de}
} 16, 22 -- Date: Fri, 28 Apr 2017 10:34:02 +0200
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From: microscopy.listserver-at-gmail.com
Date: Fri, 28 Apr 2017 11:50:29 -0500
Subject: [Microscopy] viaWWW:support films for TEM - anyone making silicon nitride or

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Email: bethrichardson-at-uga.edu Name: Beth Richardson

Organization: University of Georgia

Title-Subject: [Filtered] support films for TEM - anyone making silicon nitride or graphene films?

Message: Hi all,
I'd like to make good support films for TEM. I use to be able to make films without holes in them
but my tried-and-true method using 0.25% Formvar in ethylene dichloride hasn't been working
(purchased solution or made in-house). Holey support films have become a way of life and one of the
weakest links in my productivity. I'd like to learn more about making silicon nitride or graphene
films (they are so expensive to buy). Can anyone share a protocol? If necessary I have access to a
nice chemist and a clean room. thanks in advance for any advice,
Beth


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From: Connon.Thomas-at-mpfi.org
Date: Fri, 28 Apr 2017 14:03:46 -0500
Subject: [Microscopy] Re: Certified thickness of ultramicrotome cuts - advice needed

Contents Retrieved from Microscopy Listserver Archives
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Hi Stefan,

Work has been done to determine section thickness in a more exact way than using interference colors alone.

One method is to use the minimal folds method using the TEM (Small, 1968). Imagine crinkling a piece of paper so a fold forms in the middle in the Z axis, which will be twice the thickness of the paper. This fold can be measured on the TEM to get a relatively accurate thickness measurement. Ideally, measure multiple folds and get an average thickness. This method is addressed nicely in the following paper:

When using samples with mitochondria, you can also use the cylindrical shape of those to estimate section thickness (Fiala and Harris, 2001). This is ideal if you have sections with very few wrinkles and was found to have an average thickness measurement very similar to that of the minimal folds method.

Yet another method is to reembed some sections you've cut and section them again at 90 degrees to measure the thickness (Bedi, 1987).

Finally, laser confocal microscopes can be used to measure the section thickness with a reported accuracy of 1 nm (see Kubota et al. 2009).

Hope this helps,

Connon



Connon Thomas
Max Planck Florida Institute for Neuroscience
Electron Microscopy Technician
One Max Planck Way
Jupiter, FL 33458
Email: Connon.Thomas-at-mpfi.org


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From: wtivol-at-sbcglobal.net
Date: Sun, 30 Apr 2017 18:55:11 -0500
Subject: [Microscopy] Re: viaWWW:support films for TEM - anyone making silicon nitride or

Contents Retrieved from Microscopy Listserver Archives
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Formalin is historically ~40% formaldehyde in water and laced with 5-10% MeOH to inhibit polymerization(?).
Thus, 10% "Formalin" is 4% in Formaldehyde. That is the historically recommended concentration of the gas as a fixative. 4% paraformaldehyde at 4DegC (buffered or not) is also a very good treatment (10-20 min) for 'freezing,' muscle in thin tissues that are under various degrees of tension (e.g., in a partially to maximally distended urinary bladder), before they are immersed (or filled) with with the same fixative. Fixation at 4DegC is a very good fixative for both routine and special histology.

I stopped using Formalin in the mid 1970's, and substituted as follows.

I usually make 20% HCHO from Paraformaldehyde, and I can refrigerate it in 100ml aliquots for over a decade without any polymerization.

Formalin ought not be used for anything in biological histology, unless the MeOH is absent - which wolution should not be called "Formalin."

Cheers,

Fred Monson

Frederick C. Monson, PhD
Technical Director - until the end of 16 June, 2017.
Center for Microanalysis and Imaging, Research and Training (CMIRT)
West Chester University of PA
Geology-Astronomy
750 South Church St.
West Chester, PA, 19383
fmonson-at-wcupa.edu
610-738-0437(Work)



-----Original Message-----
X-from: barnesa-at-umn.edu [mailto:barnesa-at-umn.edu]
Sent: Wednesday, April 26, 2017 5:01 PM
To: Monson, Frederick {FMonson-at-wcupa.edu}

Technically you can heat the solution to try and depolymerize the presumptive paraformaldehyde oligomers. I do *not* recommend trying this - it works occasionally, but hot formalin is almost never a good idea even in a hood.

Option 1: Send it to Haz Waste and buy another bottle.
Option 2: Filter it and use it anyway.

I've needed to use option 2 a few times over the years - mostly due to reagent shipping issues and/or "surprise!" samples. In my hands, filtered PF works okay for straight histology-level work, so-so (at
best) for immuno work, and leads to a range of....interesting results for EM samples.

I'd still recommend option 1 - formalin is { $10/liter from Fisher. In a pinch, filtering for H&E or other optical microscopy will probably be okay.

Aaron


-----
Aaron Barnes, MD, PhD
Clinical Pathology Resident (PGY1) | Dept of Lab Medicine & Pathology University of Minnesota Medical School Dunny Lab | Dept of Microbiology & Immunology


On Wed, Apr 26, 2017 at 3:33 PM, {jkrupp-at-deltacollege.edu} wrote:
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} I have a bottle of Formalin that has a white precipitate, probably polymerized formaldehyde from being stored in the refrigerator.
}
} Is there anything I can do to return the solution to normal?
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} Thanks
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} Jon
}
} Jonathan Krupp
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From devlinrobb1-at-gmail.com Fri Apr 28 21:19:42 2017
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} On Apr 28, 2017, at 10:06 AM, microscopy.listserver-at-gmail.com wrote:
}
} I'd like to make good support films for TEM. I use to be able to make films without holes in them
} but my tried-and-true method using 0.25% Formvar in ethylene dichloride hasn't been working
} (purchased solution or made in-house).

Dear Beth,
I cannot respond to the other films mentioned in your post, but as to formvar, ethylene dichloride decomposes to release HCl, which impairs formvar films, so be sure your—or your formvar supplier’s—is fresh. Also, it has been my experience that high-humidity environments make film-making problematic, and GA can be very humid this time of year.
Yours,
Bill





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From: bozhilov-at-ucr.edu
Date: Mon, 1 May 2017 10:35:01 -0500
Subject: [Microscopy] open position for EM laboratory technician

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The Central Facility for Advanced Microscopy and Microanalysis (CFAMM) at the University of California at Riveside has an open position for electron microscopy laboratory technician. Position responsibilities include operation and routine maintenance of the electron microscopes, FIB, ancillary equipment, and all phases of sample preparation and analysis of various types of materials and tissue. This position includes assisting and training facility users in specimen preparation, instrument operation, and related techniques.

https://irecruitportal.ucr.edu/irecruit/!Controller?action=jobs_webui.show_page&page=jobs_detail&requisition_id=201704267242&profile_id=&module=jobs

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From: microscopy.listserver-at-gmail.com
Date: Wed, 3 May 2017 06:45:46 -0500
Subject: [Microscopy] viaWWW:TEM open position at INRS near Montreal, QC: Research

Contents Retrieved from Microscopy Listserver Archives
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Hi all,

We are looking to purchase a used (but working) Reichert-Jung Model FC-4E cryo stage and controller for our Ultracut E ultramicrotome. Please let me know if you have one you are interested in selling.

Thank you,

Melissa Holman
Laboratory Manager
3300 Breckinridge Blvd Suite 400
Duluth, GA 30096 770.662.8509

Visit us at: www.mvainc.com
Connect with me on: LinkedIn

---------------------------------------------------------------------------------------------------------------------------
The information in this email is confidential and may be legally privileged. It is intended solely for the addressee. If you are not the intended recipient, please delete the email and notify MVA Scientific Consultants of the transmission error.




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Email: stefano-at-soquelec.com Name: Stefano Rubino

Organization: Soquelec Limited

Title-Subject: [Filtered] TEM open position at INRS near Montreal, QC:
Research Assistant in ultrafast TEM

Message: OPEN RESEARCH-ASSISTANT POSITION
Institut National de la Recherche Scientifique (INRS)
Université du Québec
Centre Énergie Matériaux Télécommunications at Varennes, Canada


INRS-EMT is undertaking a unique project to image materials with
combined spatial and temporal resolutions that were not possible before.
In this context, INRS will hire a full-time research assistant to
develop, use and maintain several instruments that are housed in the new
Infrastructure for Advanced Imaging (IAI). These instruments include:
modified transmission electron microscopes (TEM), sample preparation
tools and pulsed lasers. In addition to these duties, the research
assistant is expected to train users and manage the facility at the user
level. The assistant will also have opportunities to participate to the
ongoing academic research in IAI.
INRS will provide all the necessary trainings to the research assistant
to use and maintain the equipment. Therefore, candidates with enthusiasm
to learn new technologies are particularly encouraged to apply.

The research assistant will report to the professor responsible for the
Infrastructure for Advanced Imaging.

Requirements

Education
• Minimum of a Bachelor’s degree in Physics, Applied and Engineering
Physics, Materials Science, Electrical Engineering, and related
disciplines. Experience
• Experience with transmission electron microscopes, lasers and optical
components, and sample preparation instruments.
Other • Ability and enthusiasm to work in a research team. • Good
communication skills.

Location

Institut national de la recherche scientifique
Centre Énergie Matériaux Télécommunications
1650, boulevard Lionel-Boulet
Varennes (Québec) J3X 1S2
(work location is 30 km’s away from downtown Montréal)


Salary/Benefits

The salary and benefits will be determined according to the
qualifications of the candidate.
How to Apply

Interested candidates should apply by submitting their CV online
(http://www.inrs.ca/english/career-opportunities). Further information
can be obtained by writing to: recrutement-at-adm.inrs.ca.


INRS is a graduate level university active in fundamental and applied
research. It brings together 150 professors and nearly 700
graduate-level students and postdoctoral fellows in four centers located
in Quebec City, Montréal, Varennes and Laval. It is consistently ranked
as one of the top universities in Canada in terms of research intensity
(grants per professors). The Infrastructure for Advanced Imaging is a
$15M project funded by the Canadian and Quebec governments through the
Canadian Foundation for Innovation.
INRS is committed to employment equality program. The University invites
women, visible minorities, ethnic minorities, Aboriginal people and
persons with disabilities to apply.


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From: kraftpiano-at-gmail.com
Date: Thu, 4 May 2017 19:22:01 -0500
Subject: [Microscopy] Unknown microbe- does anyone know what this is?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues,

We would like to draw your attention to the 2017 Yale Microscopy Workshop.

We invite you to participate in a two day workshop. This free annual event is a combination of symposium, demonstrations, technical lectures and small group practicals. It is open to the international research community and only requires your free registration.

Time: June 6 - 7th, 2017
Location: Yale University School of Medicine, The Anlyan Center (TAC), New Haven, CT
Cost: Free on-line or on-site registration
Information: https://medicine.yale.edu/lab/microscopy/ {http://microscopy.med.yale.edu/index.aspx} {https://medicine.yale.edu/lab/microscopy/%3Chttp://microscopy.med.yale.edu/index.aspx%3E}

Features:
- Symposia on image analysis and high-dimensional imaging
- Concurrent workshop on correlative live-cell STED and cryo-electron microscopy, and cryo EM techniques
- Demonstrations, technical lectures, and small group practicals.

Please see the website for free registration, list of accessible equipment, and the schedule of events.
Additional technical lectures are likely to be added soon. http://medicine.yale.edu/lab/microscopy/schedule/

We look forward to seeing you there,

The organizers,
Ann Haberman ann.haberman-at-yale.edu {mailto:ann.haberman-at-yale.edu}
Derek Toomre derek.toomre-at-yale.edu {mailto:derek.toomre-at-yale.edu}
Joerg Bewersdorf joerg.bewersdorf-at-yale.edu {mailto:joerg.bewersdorf-at-yale.edu}
Xinran Liu xinran.liu-at-yale.edu {mailto:xinran.liu-at-yale.edu}



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From sawijama5-at-gmail.com Wed May 3 21:01:18 2017
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Message-ID: {7649EAE9.693E3010-at-gmail.com}

I was looking at some pond water in a light microscope, and managed to capture this guy. Being a physicist, I have absolutely no idea what it might be, but I’m pretty sure that someone on this list will know what it is.

Here is a video that I took of it: http://www.jkraft.net/unknown-microbe.mp4

Also, if anyone knows of a good internet source of information on how to identify the random little slimy bits in pond water, I would appreciate it.

Thanks in advance,

Justin A. Kraft

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From: oshel1pe-at-cmich.edu
Date: Fri, 5 May 2017 07:45:59 -0500
Subject: [Microscopy] Re: Unknown microbe- does anyone know what this is?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Justin,

Contact the folks in Seth Tyler's lab at University of Maine – they run a "global worming" website about metazoan microfauna, but they're mostly marine.
http://globalworming.umaine-biology.net/
Pennak's "Freshwater Invertebrates of North America" is a good reference. After that, you need to get into the literature for each group, or google the group name, then click "images".

But. Squished by the coverslip and without a scale, it's hard to tell. The squishedness prevents the critter from exhibiting its true form.
There are several sets of somethings … 2 near the anterior end, just behind the mouth (on the right), one about ½ way along the body, on top of the animal and hard to see (about where the gut makes the sharp bend), and another 2 spaced equally behind this, on the "top" of the animal as it sits. This makes me think it's a seriously uncomfortable tardigrade. Too flattened by the coverslip to really show its true form. Common animals, but you need to put feet on your coverslips to give critters like this some room.

Great darkfield subjects as well as DIC. Through in a green filter below your condenser, and use the 40X DIC annulus with the 10X objective, and you'll have darkfield (100X annulus and 20/40X objective will work on some 'scopes).

Given how poorly studied these groups are, it could be a new species.

Phil
-------------
Philip Oshel
Imaging Facility Director
Biology Department
1304 Biosciences
1455 Calumet Ct.
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576 office
(989) 774-7567 lab

----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

I was looking at some pond water in a light microscope, and managed to capture this guy. Being a physicist, I have absolutely no idea what it might be, but I’m pretty sure that someone on this list will know what it is.

Here is a video that I took of it: http://www.jkraft.net/unknown-microbe.mp4

Also, if anyone knows of a good internet source of information on how to identify the random little slimy bits in pond water, I would appreciate it.

Thanks in advance,

Justin A. Kraft





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15, 54 -- From: "Oshel, Philip Eugene" {oshel1pe-at-cmich.edu}
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15, 54 -- Subject: Re: [Microscopy] Unknown microbe- does anyone know what this is?
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From: microscopy.listserver-at-gmail.com
Date: Sun, 7 May 2017 09:02:02 -0500
Subject: [Microscopy] viaWWW:HBO 100 light leak

Contents Retrieved from Microscopy Listserver Archives
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Looks like it may be a freshwater Oligochaete, a type of Annelid worm (think earthworms). Couldn't tell you what species it is though, that would take some careful keying as there are hundreds of them in North America (if that's where you are!). If you look closely, it has what are called chaetae (bristles or hairs) in bunches on its body. There are quite a few keys to freshwater creatures, here are just a few--


Stroud Water Research Center Identification Guide to Freshwater Macroinvertebrates (a nice, very basic visual guide to more common groups):
http://www.stroudcenter.org/education/MacroKey_Complete.pdf

Guide to the Freshwater Aquatic Microdrile Oligochaetes of North America (more specific to these worms):
http://www.dfo-mpo.gc.ca/Library/33909.pdf

Guide to freshwater microorganisms:
https://www.msnucleus.org/watersheds/mission/plankton.pdf

Freshwater Macroinvertebrates of Northeastern North America (A book I used in my undergrad to identify slimy bits in pond water)
https://www.amazon.com/Freshwater-Macroinvertebrates-Northeastern-North-America/dp/0801496888/ref=pd_lpo_sbs_14_t_1?_encoding=UTF8&psc=1&refRID=GE75176NY822DJV8BM3Y


As was previously stated, it may be helpful to reach out to a freshwater invertebrate lab if you are still curious. Hope this helps!!!!

Cheers,

Connon Thomas
Max Planck Florida Institute for Neuroscience
Electron Microscopy Technician
One Max Planck Way
Jupiter, FL 33458
Email: Connon.Thomas-at-mpfi.org


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Title-Subject: [Filtered] HBO 100 light leak

Message: I am searching on literature about the potential hazards of
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From: richard.ross-at-allisontransmission.com
Date: Mon, 8 May 2017 09:50:06 -0500
Subject: [Microscopy] viaWWW:HBO 100 light leak

Contents Retrieved from Microscopy Listserver Archives
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-------- Forwarded Message --------

Mike,
We used HBO 100 lamps in out Reichert MeF3 metallograph for several decades. Reflected light out of the illuminator cooling vents or leaking light at the illuminator/microscope joint was never cautioned against; this was 30+ years ago, but I suppose the explanation from the factory trainer was that the internal surfaces were sufficiently poor optical and UV reflectors that once the light was reflected it wasn't a concern. Of course, the potential for permanent retina damage made direct viewing of the lamp an absolute prohibition - never ignite the bulb without the protective cover in place or look directly into the optical path. That's somewhat obvious to us, but the general public doesn't comprehend the extreme brightness of these lamps.
Rick

Richard A. Ross
Sr. Metallurgist, Materials Engineering
Allison Transmission Inc.

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Message: I am searching on literature about the potential hazards of having light leaking out of an HBO 100 illuminator. There is plenty about how dangerous a mercury discharge can be if a lamp explodes.
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From: Levina.Dear-at-health.nsw.gov.au
Date: Mon, 8 May 2017 18:07:53 -0500
Subject: [Microscopy] LaB6 filaments/ cathode problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello microscopy community,

Regarding LaB6 filaments/ cathodes-

Has anyone experienced problems with LaB6 cathodes in their TEM e.g. high dark current, beam instability? I have a vague recollection of historic issues mentioned on the List server.

The two LaB6 that we purchased in 2014 and recently installed in our CM10 FEI TEM are extremely problematic. The first one caused an array of issues notably high dark current, beam instability flashing/discharging. Much trouble shooting searching was done by lab staff and FEI service engineers, cleaning of anode, wehnelts and insulator, replenishing gun insulating oil to rule out other potential causes. We also tried the spare LaB6 cathode which demonstrated the same issues.
Installing a tungsten as a last resort resulted in the EM quickly returning back to usable status with none of these issues.

I have used Lab6 from this manufacturer for years without any problems. Perhaps it is time to move on to another.

I would be interested to hear from others experiencing these or similar issues.

Levina


This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender.

Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities.



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From: microscopy.listserver-at-gmail.com
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Subject: [Microscopy] viaWWW:postion - for Microsocopy, Metrology, Microfabrication -

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From: protrain-at-emcourses.com
Date: Tue, 9 May 2017 04:12:02 -0500
Subject: [Microscopy] LaB6 filaments/ cathode problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

LaB6 units are extremely dependant on vacuum level for their stability.
Looking at the tests you have run I would be interested in the vacuum in the
gun, not as given by the microscope but by monitoring as near as possible to
the gun area, perhaps moving a microscope gauge nearer to that area if
possible?

Steve

Steve Chapman FRMS
Protrain for Consultancy and Training in Electron Microscopy
+44 (0)7711 606967
web www.emcourses.com



-----Original Message-----
X-from: Levina.Dear-at-health.nsw.gov.au [mailto:Levina.Dear-at-health.nsw.gov.au]
Sent: 09 May 2017 00:09
To: protrain-at-emcourses.com

Hello microscopy community,

Regarding LaB6 filaments/ cathodes-

Has anyone experienced problems with LaB6 cathodes in their TEM e.g. high
dark current, beam instability? I have a vague recollection of historic
issues mentioned on the List server.

The two LaB6 that we purchased in 2014 and recently installed in our CM10
FEI TEM are extremely problematic. The first one caused an array of issues
notably high dark current, beam instability flashing/discharging. Much
trouble shooting searching was done by lab staff and FEI service engineers,
cleaning of anode, wehnelts and insulator, replenishing gun insulating oil
to rule out other potential causes. We also tried the spare LaB6 cathode
which demonstrated the same issues.
Installing a tungsten as a last resort resulted in the EM quickly returning
back to usable status with none of these issues.

I have used Lab6 from this manufacturer for years without any problems.
Perhaps it is time to move on to another.

I would be interested to hear from others experiencing these or similar
issues.

Levina


This message is intended for the addressee named and may contain
confidential information. If you are not the intended recipient, please
delete it and notify the sender.

Views expressed in this message are those of the individual sender, and are
not necessarily the views of NSW Health or any of its entities.



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From: henning.stahlberg-at-unibas.ch
Date: Wed, 10 May 2017 05:06:47 -0500
Subject: [Microscopy] Introducing FOCUS: The interface between data collection and data

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Cryo-EM Users,

We would like to draw your attention to a new software system called FOCUS, which we find quite handy to work together with automated cryo-EM data collection, as done for example with SerialEM.

FOCUS is a C++ / Qt front end, running own and third-party softwares in the background. It features a GUI that allows you to define and edit parameters and C-shell or Python scripts, which are then executed in queues. Users can add or edit their own scripts, to adapt FOCUS to specific workflows or tasks. FOCUS comes with a set of default scripts, ready to run.
Installation of FOCUS requires also the installation of any third-party programs that you want FOCUS to utilise, such as IMOD, EMAN, FREALIGN, or MotionCor2, UNBLUR, Zorro, CTFFIND4, gCTF, gAutomatch, or others.

If you install FOCUS on a strong Linux machine on a computer adjacent to the automated SerialEM run, then FOCUS can for example be used to:
- monitor the file system of the SerialEM run
- fetch newly recorded movies from the SerialEM computer, together with the pixel-defect-list and gain reference files,
- unpack the compressed movies (TIFF ZLW), gain correct,
- Fourier crop 8k to 4k, or to any pixel size you want (optionally)
- drift-correct (with ZORRO, MotionCor2, or Unblur)
- computer 2D averages of the movie, and FFTs of all files
- measure defocus (gCTF or CTFFIND4)
- pick particles (gAutomatch)
- present the last recorded image and its FFT (before and after drift correction), and the drift trajectory plot and CTF Thon ring plot on a website that you would have to setup (ours is at https://status.c-cina.unibas.ch. Ours is open and shows blurred images, but you can protect it with a password and then display the crisp images)
- present the statistics of each recorded image on that web site (as plots over time)
- organize all recorded movies in a large table (similar to an Excel sheet), which can be sorted by various parameters such as
-- grey value (which images are too dark (contaminated, beam lost) or too white (empty hole?)
-- defocus (which images are in focus or totally out of focus?)
-- CTF resolution (for which images is CTF determination difficult?)
-- iciness (which images have a too high ratio of crystalline ice, or show devitrified sample?)
-- drift (which images have too long or too short or too jittery drift trajectories? which images have too high remaining interframe drift?)
-- and various other parameters.
This table can be sorted by any of such parameters, and images can be moved into a TRASH folder with one click.
- remaining images can be exported in folder structures and STAR files ready for subsequent processing in, e.g., Relion.

FOCUS can also be used for tomography sessions, to:
- recognize parameters from the file name (e.g., specimen number and tilt angle) and organize files accordingly
- drift-correct each recorded movie at a certain tilt angle, while taking the current electron dose and the prior electron dose from earlier tilt angle recordings on the same specimen into account for electron-dose-dependent B-factor resolution filtering
- compute one 2D image per tilt angle movie
- re-organize the tilt angle images by tilt angle (-60,-59,…,0,…,59,60) when recorded in the Hagen scheme (0,1,-1,-2,2,3,-3,-4,4,5,…60,-60)
- as above: monitor data collection progress on a web site, sort data, assist in manually pruning of data,
- export data in MRC stacks with one frame per tilt angle.
- write out metadata for the stacks into STAR files

FOCUS can also be used for 2D crystal sessions, where it can do the entire 2D crystal processing automatically (the full functionality of the 2dx software package is included in FOCUS). This allows direct 3D reconstructions during the 2D crystal data collection session.

FOCUS maintains a batch queue, with which is runs all processes above in parallel.

We have installed this on an Ubuntu PC with
2x12 cores (= 48 threads)
256 GB RAM (of which 96GB are used as a RAM disk. More would make life easier.)
70 TB HD (RAID5)
2 x GTX1080 GPU
We also use a 40” 4k monitor, which is great to display the images full-screen in full quality.
We have one such system per automated cryo-EM microscope, which is able to keep up with single particle data collection of 40-frame 8k movies at a rate of 90 movies per hour (Fourier cropping, drift correction wth MotionCor2 on 5x5 patches, CTFFIND4, gautomatch, etc.), and is thereby on par with data collection.

FOCUS is described in Biyani et al., J. Struct. Biol., 2017 (https://www.ncbi.nlm.nih.gov/pubmed/28344036), also available on bioRxiv at https://doi.org/10.1101/105452.
The software is open-source and freely available in precompiled versions for Linux and OS X. A manual in wiki form is available, all at http://focus-em.org.

Nikhil Biyani, Ricardo D. Righetto, Robert McLeod, Daniel Caujolle-Bert, Daniel Castano-Diez, Kenneth N. Goldie, and Henning Stahlberg

Henning Stahlberg, PhD
Prof. for Structural Biology, C-CINA, Biozentrum, University Basel
Mattenstrasse 26 | D-BSSE | WRO-1058 | CH-4058 Basel | Switzerland
http://c-cina.org | Tel. +41-61-387 32 62





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15, 67 -- Subject: Introducing FOCUS: The interface between data collection and data
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From: microscopy.listserver-at-gmail.com
Date: Fri, 12 May 2017 06:46:10 -0500
Subject: [Microscopy] viaWWW:Job Posting-- Electron Microscopy Technician II

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Not having seen any replies so far, I will jump in.

I had an IXRF system for over ten years; indeed, we were one of the early customers. However, I cannot recall the specifics of their MDL numbers.

MDL should mean the actual lower detectable concentration given the parameters of the collection. Therefore, it should drop with additional counts because the relative noise in the background is being reduced. If it does not change over time, then it could be a defined parameter which would not be correct in my opinion.

You may also want to look into the practicalities of their system and its calculations. (Remember, my information is several years out of date.) An old version of their software fitted the background and subtracted it and then rectified the data. That is, all of the negative counts in the spectrum were set to zero. That resulted in a positive integral and result for virtually any element even when none was present. The reported amount diminished over time (approached zero) for those cases where the element was not present. It may have been that the reported level was less than the reported MDL. I should have compared the number to the MDL and ignored results less than MDL. (Many systems flag results which are less than statistically significant, but some do it more clearly than others.) However, I think it would have been better if they had let the result wander around zero (both positive and negative) and settled in closer and closer to zero with time. I have heard from various software people that negative results are incomprehensible for many users so they (the developers) take steps which end up biasing the results.

For the record, I reported this matter to IXRF on at least two occasions and it had not been resolved the last time I checked which was several years back. I was told it was going to be addressed. I certainly hope it has been fixed by now, but we may all be able to cite software issues that languish on the do-do list. Maybe one of their engineers is following the list and can clarify the matter.

Warren Straszheim

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Organization: Charles River Laboratories
Title-Subject: [Filtered] Job Posting-- Electron Microscopy Technician II

Message: DEPARTMENT NAME: EM Laboratory
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COMPANY SUMMARY:
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Will also assist with inventory, maintenance of equipment, report writing, QC. Our work is
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From: microscopy.listserver-at-gmail.com
Date: Fri, 12 May 2017 06:47:02 -0500
Subject: [Microscopy] viaWWW:postion - for Microsocopy, Metrology, Microfabrication -

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Email: pchapman-at-latech.edu Name: Phillip Chapman

Organization: Louisiana Tech University

Title-Subject: [Filtered] postion - for Microsocopy, Metrology, Microfabrication - Louisiana Tech
University

Message: Institute for Micromanufacturing is looking for Research Assistant Professor (non-tenure
track)for Microscopy, Metrology, and Microfabrication.

Applicants must have a Ph.D. degree or equivalent in chemistry, physics, material science,
engineering, or related technical field.

For details and application instructions please go to the Louisiana Tech University employment web
site or directly to the posting at: http://finance.latech.edu/hr/vacan2561.php


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From: microscopy.listserver-at-gmail.com
Date: Fri, 12 May 2017 06:47:38 -0500
Subject: [Microscopy] viaWWW:Newby- recently acquired Jeol 5310 SEM needs schematics

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Email: desertrw1-at-gmail.com
Name: Russ Winstead

Organization: Self, Sandia Scientific, Inc

Title-Subject: [Filtered] Newby- recently acquired Jeol 5310 SEM needs schematics

Message: Hi- I am new to the microscopy list server, and I am excited about getting involved in this
arena!

I recently purchased a surplus Jeol 5310 SEM that I am currently learning to use, I previously
helped restore an older Jeol SEM about 15 years ago. The 5310 had an issue with the HT (High Tension
enable) button not working after initial vacuum pumpdown. After tracing the button wires I found
that somebody had spilled some coffee (appearance anyway) onto the circuit boards beneath the
console. I unsoldered several components, cleaned the boards, and re-installed the components- HT
has worked perfectly since then, I have been enjoying lots of close-ups of various watches, tiny
filaments, etc.
I am also planning to replace the original polaroid camera with a digital camera system, for now I
simply take pictures of the main CRT display. Has anybody done this?

I am fearful that I will need the electrical schematics someday as this microscope becomes older. I
do not have any of the original documentation, it was somehow lost before I purchased it! I am
hoping that somebody PLEASE PLEASE can make a copy of their operation manuals and schematics for the
Jeol 5310?? I would be happy to compensate or purchase... Any documentation that is associated with
the Jeol 5310 SEM would be appreciated!

I also would like to see if anybody has a sputter coater or carbon coater that needs repair or can
be purchased for a reasonable price- I do not have either right now and I think bugs and other
subjects are in my future!

I also have about 20 extra "K type" filaments (some used, some new) for the Jeol, maybe some type of
trade or if anybody needs a few...

Comments, suggestions, and ideas are welcome- thanks in advance!

Russ

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From: microscopy.listserver-at-gmail.com
Date: Fri, 12 May 2017 17:20:28 -0500
Subject: [Microscopy] viaWWW:Zeiss CEM 902 TEM Repair Recommendations

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Email: areplogle-at-pugetsound.edu

Name: Amy Replogle

Organization: University of Puget Sound

Title-Subject: [Filtered] TEM Repair Recommendations

Message: Hi everyone,

We have a Zeiss CEM 902 Transmission Electron Microscope that is currently displaying the following
error code:

3-1: Output voltage of high-voltage measuring tube below 0.1V (broken measuring line, defective
measuring instrument, or contaminated measuring tube does not ignite).
Zeiss no longer supports this instrument so we are looking for recommendations for maintenance and
repair. Any suggestions?
Thanks,
Amy

---------------------------------------------------
Amy Replogle '05
Biology Department
Adjunct Assistant Professor
Science Core Facility Technician


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From: peter.heimann-at-uni-bielefeld.de
Date: Sat, 13 May 2017 06:19:13 -0500
Subject: [Microscopy] Re: Zeiss CEM 902 TEM Repair Recommendations

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Amy,

when does the error code light up? only after quite a while when the EM
was running?

I heard of a problem that in rare cases the vacuum becomes too good (
e.g. better than 10 minus 7) and thus the voltage exceeds the minimal
value which is necessary. The people helped themselves via a needle
valve continuously admitting a smallest amount of air.

good luck,

Peter ( happy user of Zeiss EM109 TEM)

++++++++++++++++++++++++++++++++++++++++++++++++


Am 13.05.2017 um 00:26 schrieb microscopy.listserver-at-gmail.com:
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} Email: areplogle-at-pugetsound.edu
}
} Name: Amy Replogle
}
} Organization: University of Puget Sound
}
} Title-Subject: [Filtered] TEM Repair Recommendations
}
} Message: Hi everyone,
}
} We have a Zeiss CEM 902 Transmission Electron Microscope that is currently displaying the following
} error code:
}
} 3-1: Output voltage of high-voltage measuring tube below 0.1V (broken measuring line, defective
} measuring instrument, or contaminated measuring tube does not ignite).
} Zeiss no longer supports this instrument so we are looking for recommendations for maintenance and
} repair. Any suggestions?
} Thanks,
} Amy
}
} ---------------------------------------------------
} Amy Replogle '05
} Biology Department
} Adjunct Assistant Professor
} Science Core Facility Technician
}
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} Login Host: 207.207.127.230
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9, 25 -- From peter.heimann-at-uni-bielefeld.de Sat May 13 06:19:06 2017
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From: diller-at-stefan-diller.com
Date: Sat, 13 May 2017 13:58:58 -0500
Subject: [Microscopy] Re: viaWWW:Zeiss CEM 902 TEM Repair Recommendations

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Ami,

first it would be useful to know if the error comes from a Pirani or a Penning tube. Can you be more specific? Can you relate the
error code with the location / kind of the measuring tube?

If Penning, I would first try to clean it. If the Penning is too contaminated, it looks like the vacuum is ultra-good ;-)

Procedure would be to set the part of the vacuum system the tube is fixed at on air, then switch off the mic completely, since
there might be HIGH voltage on the tube.

You can send me an image of the measuring tube(s) and I can check if I have a working one to send over.


Best,

Stefan

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} We have a Zeiss CEM 902 Transmission Electron Microscope that is currently displaying the following
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From: ajhall-at-prairienanotech.com
Date: Sat, 13 May 2017 22:59:47 -0500
Subject: [Microscopy] Popular, Inspiring University of Illinois Scientist and Nanotechnology

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

It is with a heavy heart that we have to announce the passing of an
advisory board member, and pioneer in SPM analysis, Dr. Scott W.
Maclaren. He will be sorely missed as a fellow scientist and a close friend.

A picture of Scott, one of his popular SPM images, and the following
article can be found here:
https://www.linkedin.com/pulse/popular-inspiring-university-illinois-scientist-pioneer-allen-hall

One of the most popular and beloved scientists in the Midwest, who
trained more than a thousand researchers and inspired creativity in
thousands more, has died. Dr. Scott Maclaren, of the University of
Illinois at Urbana–Champaign (UIUC), succumbed to complications related
to diabetes on Tuesday, May 9th. He was 57.

Maclaren served as Senior Staff Scientist of UIUC’s Frederick Seitz
Material Research Laboratory. The son of a General Electric executive,
born in Syracuse, New York and raised in Chagrin Falls, Ohio, Maclaren
was a childhood prodigy who showed an early aptitude in science and
began taking university courses while still in high school. He attended
the prestigious Massachusetts Institute of Technology (MIT) where he
mastered several disciplines; taking on a double major in Physics and
Planetary Science. While at MIT, Maclaren also taught physics to
underclassmen and earned a reputation for his inspiring instruction
style. He graduated in 1982 after writing a thesis on laser beam
weaponry that several of his teachers were concerned might have to be
classified.

Shortly after leaving MIT, Maclaren moved to UIUC where he remained for
the rest of his life. Although he was considered an expert in many
scientific disciplines, the field Maclaren was most famous in was
Scanning Probe Microscopy, or SPM. Basically, a form of imaging of
materials at the atomic level and used extensively in the emerging field
of nanotechnology.

Maclaren’s knowledge and experience commanded global respect and his
expertise was much sought after by many foreign institutions. Maclaren
also worked as an advisor to several major companies in the United
States and aboard. Among them were US-based Asylum Research and
BudgetSensors of Bulgaria. Maclaren was also an advisor and mentor to
Illinois-based Prairie Nanotechnology. Aside from giving lectures and
tutorials on SPM at many national workshops and conferences, Maclaren
was also involved in several outreach programs promoting nanotechnology
in science education.

Even though Maclaren was plagued by numerous health issues in the latter
part of his life, he still had a work ethic those around him sometimes
found intimidating. Every weekend, Maclaren could be found in MRL’s
Atomic Force Microscopy lab investigating any material he found
interesting and juggling multiple projects.

After being checked into Presence Convent Medical Center in Urbana on
May 3rd for what seemed to be a minor skin condition, his health quickly
deteriorated. He died shortly thereafter.

Funeral arrangements are currently being finalized and will be private.
A traditional memorial service unique to UIUC will be held later this
year. In lieu of flowers, the family requests that donations be made in
his memory to the Orpheum Children’s Science Museum (orpheumkids.net) or
the Vasculitis Foundation (vasculitisfoundation.org).

With my sincere condolences to all of Scott's friends and colleagues, he
will be sorely missed.
-Allen J. Hall
http://www.prairienanotech.com/


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From: microscopy.listserver-at-gmail.com
Date: Mon, 15 May 2017 15:40:12 -0500
Subject: [Microscopy] viaWWW:3rd International workshop on TEM spectroscopy in materials

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Thank you for all the advice re LaB6 filaments.

My original message;
Has anyone experienced problems with LaB6 cathodes in their TEM e.g. high dark current, beam instability? I have a vague recollection of historic issues mentioned on the List server.

The two LaB6 that we purchased in 2014 and recently installed in our CM10 FEI TEM are extremely problematic. The first one caused an array of issues notably high dark current, beam instability flashing/discharging. Much trouble shooting searching was done by lab staff and FEI service engineers, cleaning of anode, wehnelts and insulator, replenishing gun insulating oil to rule out other potential causes. We also tried the spare LaB6 cathode which demonstrated the same issues.
Installing a tungsten as a last resort resulted in the EM quickly returning back to usable status with none of these issues.

I have used Lab6 from this manufacturer for years without any problems. Perhaps it is time to move on to another.

I would be interested to hear from others experiencing these or similar issues.
---------------------------------------------------------------------------------------------------------------------------------


Summary of some of the responses;
-cleaned the legs of the filament (emery paper then washed with acetone) resolved the problems.
-that LaB6 filaments have a limited shelf life. (I have not seen a use by date on any that we have purchased)
-extremely dependant on vacuum level for their stability.
-LaB6 filaments, from crystals cleaving to bases coming loose - and it seems from multiple suppliers.

Result; A new LaB6 was purchased from the same manufacturer, it was installed and is working beautifully, so good to see such a bright green glow again - problem fixed!

I am waiting to hear back from the manufacturer regarding the two LaB6 that were returned.

Kind regards
Levina



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Email: ling.xie-at-angstrom.uu.se Name: Ling Xie

Organization: Uppsala University

Title-Subject: [Filtered] 3rd International workshop on “TEM
spectroscopy in materials science”
Message: Dear Microscopists,

We organize the 3rd international workshop on “TEM spectroscopy in
materials science” in Uppsala (19th-22th June), Sweden. We have invited
a number of colleagues with a long and excellent background from the
field of TEM spectroscopy and have made strong efforts to keep the
registration fees very low (150 euros for Students, 200 Euros for
Researchers). We believe that this could make the workshop interesting
for some of your co-workers or students. Uppsala is the closest city to
Stockholm airport (18min) and the airport is easy to reach for national
and international flights.
Early bird registration deadline is 19th of May, this friday!
Please, would you be so kind and forward the attached flyer and hang it
up in your corridor? We would be very pleased, if we could meet you and
your group members on the workshop!

If you have any questions, please send an e-mail to:
tem-workshop-at-angstrom.uu.se

Best regards,
Organization committee


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From: microscopy.listserver-at-gmail.com
Date: Mon, 15 May 2017 17:36:51 -0500
Subject: [Microscopy] viaWWW:How to change the EELS acquisition area on CCD

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Email: hasan.ali-at-angstrom.uu.se Name: Hasan Ali

Organization: Uppsala University

Title-Subject: [Filtered] How to change the EELS acquisition area on CCD

Message: In electron energy loss spectroscopy, only a small central part of the CCD, vertical to the
energy dispersion axis, is used to acquire the spectrum. If I acquire the camera image of EELS, I
get such kind of spectrum on the CCD. For my application, I want to extend the vertical range of
EELS on the CCD. I changed the camera coordinates in the "EELS acquire" setup, but it doesn't change
the acquisition area of EELS on CCD. Can someone help me to know how can I change the area of the
CCD used for EELS acquisition? I am usin a GIF2002.
Thanks

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From: donc-at-asmicro.com
Date: Tue, 16 May 2017 15:22:41 -0500
Subject: [Microscopy] Re: [a] Popular, Inspiring University of Illinois Scientist and Nanotechnology

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This is a shock to me. I always enjoyed talking with Scott and visiting his
lab.
regards,
Don
=============================================
Don Chernoff, Ph.D., President
Advanced Surface Microscopy, Inc.; 3250 N. Post Rd., Suite 120;
Indianapolis IN 46226 USA
E-Mail: donc-at-asmicro.com www.asmicro.com
Voice: +1-317-895-5630 Toll free: +1-800-374-8557 (in USA & Canada)
Fax: +1-317-895-5652
[business activities since 1990: analytical services in AFM, AFM probes,
consulting, training, calibration and test specimens,
calibration and measurement software, used NanoScope equipment.]
=============================================
----- Original Message -----
From: ajhall-at-prairienanotech.com
To: donc-at-asmicro.com
Sent: Sunday, May 14, 2017 1:17 AM
Subject: [a] [Microscopy] Popular, Inspiring University of Illinois
Scientist and Nanotechnology





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It is with a heavy heart that we have to announce the passing of an
advisory board member, and pioneer in SPM analysis, Dr. Scott W.
Maclaren. He will be sorely missed as a fellow scientist and a close
friend.

A picture of Scott, one of his popular SPM images, and the following
article can be found here:
https://www.linkedin.com/pulse/popular-inspiring-university-illinois-scientist-pioneer-allen-hall

One of the most popular and beloved scientists in the Midwest, who
trained more than a thousand researchers and inspired creativity in
thousands more, has died. Dr. Scott Maclaren, of the University of
Illinois at UrbanabChampaign (UIUC), succumbed to complications related
to diabetes on Tuesday, May 9th. He was 57.

Maclaren served as Senior Staff Scientist of UIUCbs Frederick Seitz
Material Research Laboratory. The son of a General Electric executive,
born in Syracuse, New York and raised in Chagrin Falls, Ohio, Maclaren
was a childhood prodigy who showed an early aptitude in science and
began taking university courses while still in high school. He attended
the prestigious Massachusetts Institute of Technology (MIT) where he
mastered several disciplines; taking on a double major in Physics and
Planetary Science. While at MIT, Maclaren also taught physics to
underclassmen and earned a reputation for his inspiring instruction
style. He graduated in 1982 after writing a thesis on laser beam
weaponry that several of his teachers were concerned might have to be
classified.

Shortly after leaving MIT, Maclaren moved to UIUC where he remained for
the rest of his life. Although he was considered an expert in many
scientific disciplines, the field Maclaren was most famous in was
Scanning Probe Microscopy, or SPM. Basically, a form of imaging of
materials at the atomic level and used extensively in the emerging field
of nanotechnology.

Maclarenbs knowledge and experience commanded global respect and his
expertise was much sought after by many foreign institutions. Maclaren
also worked as an advisor to several major companies in the United
States and aboard. Among them were US-based Asylum Research and
BudgetSensors of Bulgaria. Maclaren was also an advisor and mentor to
Illinois-based Prairie Nanotechnology. Aside from giving lectures and
tutorials on SPM at many national workshops and conferences, Maclaren
was also involved in several outreach programs promoting nanotechnology
in science education.

Even though Maclaren was plagued by numerous health issues in the latter
part of his life, he still had a work ethic those around him sometimes
found intimidating. Every weekend, Maclaren could be found in MRLbs
Atomic Force Microscopy lab investigating any material he found
interesting and juggling multiple projects.

After being checked into Presence Convent Medical Center in Urbana on
May 3rd for what seemed to be a minor skin condition, his health quickly
deteriorated. He died shortly thereafter.

Funeral arrangements are currently being finalized and will be private.
A traditional memorial service unique to UIUC will be held later this
year. In lieu of flowers, the family requests that donations be made in
his memory to the Orpheum Childrenbs Science Museum (orpheumkids.net) or
the Vasculitis Foundation (vasculitisfoundation.org).

With my sincere condolences to all of Scott's friends and colleagues, he
will be sorely missed.
-Allen J. Hall
http://www.prairienanotech.com/


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From: microscopy.listserver-at-gmail.com
Date: Thu, 18 May 2017 07:30:20 -0500
Subject: [Microscopy] viaWWW:Research Associate I - electron microscopy

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Email: zluo-at-uncfsu.edu Name: Zhiping Luo

Organization: Fayetteville State University

Title-Subject: [Filtered] EPMA/SEM staff position available

Message: A permanent staff position in EPMA/SEM is immediately available at Fayetteville State
University, North Carolina.

Application should be submitted at https://jobs.uncfsu.edu/postings/15287.

More info can be found at http://www.uncfsu.edu/sencr-mic.

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From irapier044-at-gmail.com Wed May 17 11:08:34 2017
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Email: Gail.Phillips-at-umassmed.edu
Name: Gail Phillips

Organization: University of Massachusetts Medical School

Title-Subject: [Filtered] Research Associate I - electron microscopy

Message: The Department of Neurobiology is seeking a research associate I with electron microscopy
experience. Interested candidates should go to the link below and apply:

https://www.ummsjobs.com/job/2298/



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From: microscopy.listserver-at-gmail.com
Date: Fri, 19 May 2017 07:45:37 -0500
Subject: [Microscopy] viaWWW:Job opening at Stanford University: Transmission Electron

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: tobi-at-stanford.edu

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Email: tobi-at-stanford.edu
Name: Tobi Beetz

Organization: Stanford University

Title-Subject: [Filtered] Job opening at Stanford University: Transmission Electron Microscopy
Scientist, Stanford Nano Shared Facilities

Message: Transmission Electron Microscopy Scientist, Stanford Nano Shared Facilities

The Stanford Nano Shared Facilities (SNSF) is seeking a Transmission Electron Microscopy (TEM)
scientist to lead the operations of the facilities’ FEI Titan Environmental TEM with spherical
aberration corrector. The TEM scientist will operate, maintain, and optimize the microscope. He/She
will provide training and support to researchers, develop and implement advanced techniques, and
engage in research activities.

Stanford’s shared nanofacilities offer a comprehensive array of advanced nanofabrication and
nanocharacterization tools. Over 1,000 researchers make use of the shared facilities each year in
order to further their research programs. The goal of the shared facilities at Stanford University
is to provide open, cost-effective access to state-of-the-art nanofabrication and
nanocharacterization facilities for scientists and engineers from academia, small and large
companies, and government laboratories. The FEI Titan TEM is organized under SNSF in the Electron &
Ion Microscopy Suite which currently features a FEI Tecnai TEM as well as two FIB/SEMs and two SEMs.
The TEM scientist will report to the Faculty Director of SNSF. For more information about SNSF,
visit http://snsf.stanford.edu.

https://stanfordcareers.stanford.edu/job-search?jobId=75014


Also still accepting applications for Scanning Probe Microscopy Laboratory Manager, Stanford Nano
Shared Facilities, https://stanfordcareers.stanford.edu/job-search?jobId=74618



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From: microscopy.listserver-at-gmail.com
Date: Fri, 19 May 2017 18:01:07 -0500
Subject: [Microscopy] viaWWW:ImageStream course

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: leandro.lemgrubersoares-at-glasgow.ac.uk


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Email: leandro.lemgrubersoares-at-glasgow.ac.uk Name: Leandro Lemgruber

Organization: Wellcome Centre for Molecular Parasitology - University of Glasgow

Title-Subject: [Filtered] ImageStream course

Message: Dear All

on August we will have a symposium here in Glasgow on the use of ImageStream in the fields of
immunology and parasitology. To register and information about the event -
https://www.eventbrite.co.uk/e/imagestream-symposium-tickets-32725310284

Feel free to advertise within your institutions.

Best

Leandro

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From: microscopy.listserver-at-gmail.com
Date: Fri, 19 May 2017 18:02:03 -0500
Subject: [Microscopy] viaWWW:question regarding; K-kit fior TEM?

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Email: ravithakkar-at-vet.k-state.edu Name: Ravindra Thakkar

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Title-Subject: [Filtered] question regarding; K-kit

Message: Hi Listeners,
If any body here has used K-kit to analyse liquid sample under TEM. Is k-kit useful for liposome
too? What kind of specimen holder needed? Will FEI Double tilt holder works?

Ravi.

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From: erodek-at-2spi.com
Date: Mon, 22 May 2017 10:43:51 -0500
Subject: [Microscopy] viaWWW:question regarding; K-kit fior TEM?

Contents Retrieved from Microscopy Listserver Archives
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Hello Ravi:

(1) If anybody here has used K-kit to analyze liquid sample under TEM.

We have worked with the K-kit system in our facility as well as working with others who are using the K-kit for a number of liquid systems.

(2) Is k-kit useful for liposome too?

In general, if a k-kit loaded with organic substance that without staining, the imaging results mostly would suffer low contrast under TEM. The liposome is just one of the examples.

A liposome has an aqueous solution core surrounded by a thin hydrophobic membrane, in the form of a lipid bilayer. Due to the contrast issue, the TEM images for some smaller sizes of liposomes are easily looked blurry, and poor for being clearly identified. Per our actual test results, we suggest that Cryo-TEM can be a better option for those smaller liposomes. However, one also can use K-kits for the observations of some larger liposomes under TEM.

(3) What kind of specimen holder needed?

Since K-kit is mounted on a standard copper grid for TEM observation, it's sure compatible with most kinds of TEM Holder.

(4) Will FEI Double tilt holder works?

Some kinds of FEI double tilt holders require using a small screwed cover ring to lock the copper grid fixed at the holder's front stage. It usually needs to use a dedicated tool which with a small hexagonal head to fasten them together. In this case, due to K-kit body on the copper grid is too thick, it will make the tool failed for screwing down the cover ring.

I hope this information has been of some help.

Regards

Gene Rodek

Eugene Rodek
Vice President
SPI Supplies
206 Garfield Ave, West Chester, PA 19380
610-436-5400 (P), 610-436-5755 (F)
erodek-at-2spi.com
www.2spi.com


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Title-Subject: [Filtered] question regarding; K-kit

Message: Hi Listeners,
If any body here has used K-kit to analyse liquid sample under TEM. Is k-kit useful for liposome too? What kind of specimen holder needed? Will FEI Double tilt holder works?

Ravi.

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From: d.v.korneev-at-ngs.ru
Date: Mon, 22 May 2017 11:26:15 -0500
Subject: [Microscopy] looking for a postdoctoral position in physics and microscopy

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Dear colleagues,

I defended my Ph.D. thesis in October 2016 and now I am
looking for a postdoctoral position in microscopy (AFM,
TEM, SEM) and biophysics of microorganisms(especially,
viruses, I like them :)).
If there is an open position in your lab, please write me.

Profile in ResearchGate
https://www.researchgate.net/profile/Denis_Korneev

Profile in Google Scholar
http://scholar.google.ch/citations?user=xW-kLNoAAAAJ&hl

Thank you in advance!
Denis

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From: microscopy.listserver-at-gmail.com
Date: Tue, 23 May 2017 07:59:29 -0500
Subject: [Microscopy] viaWWW:Hitachi FIB and STEM English manuals

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Email: dcristofori-at-unive.it Name: Davide Cristofori

Organization: "Ca' Foscari" Ujiversity of Venice

Title-Subject: [Filtered] Hitachi FIB and STEM English manuals

Message: Dear Listers,
Due to some stupid promotional image automatically attached to mails by our mail system, I'm bound
to write you via web form.

Can anyone provide me the English manuals of the two following machines?

FIB-SEM Hitachi FB2200
STEM/SEM Hitachi HD-2700

Thank you in advance for your help.
Regards

Davide

~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~
Davide Cristofori

direct phone = 041.234.67.26
e-mail = dcristofori[at]unive[dot]it

Centro di Microscopia Elettronica "Giovanni Stevanato" e
Dipartimento di Sc. Molecolari e Nanosistemi
Universit Ca' Foscari Venezia

Campus Scientifico, Edificio ETA
Via Torino 155 I-30172 Mestre (VE) Italy
~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~

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From: microscopy.listserver-at-gmail.com
Date: Thu, 25 May 2017 08:35:35 -0500
Subject: [Microscopy] viaWWW:Job Opening - Cryo-EM Specialist

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Email: fitzp-at-wustl.edu Name: James Fitzpatrick

Organization: Washington University School of Medicine

Title-Subject: [Filtered] Job Opening - Cryo-EM Specialist

Message: Dear List.

I have a new position open in my group for a Cryo-Electron Microscopy Specialist. This role will
support the operation of my new Titan Krios G3 Cryo-EM which is equipped with a K2 Direct Electron
Detector for single particle averaging and cellular tomography studies.
The position will work with other EM staff under my supervision in operation of the electron
microscopy component of my group - Wash U Center for Cellular Imaging (http://wucci.wustl.edu/). The
Cryo-EM Specialist will also have the opportunity to work on correlative microscopy technology
development projects with other members of the group.


PRIMARY DUTIES AND RESPONSIBILITIES:

User Training - train users in sample preparation and imaging techniques for single-particle cryo-EM.

Cryo-EM Service - Prepare samples for single particle cryo-EM and collect and reconstruct images for
University researchers to help to prepare data for publication and presentations.

Cryo-EM Equipment Maintenance - maintenance of the cryo-EM and sample preparation equipment.


QUALIFICATIONS

At least 2-5 years of cryo-EM experience, with expertise in sample freezing, sample handling,
microscope use, and data evaluation and analysis.

Firm understanding of the principles of Transmission Electron Microscopy (TEM)

Demonstrated skill and experience in the high-resolution operation of cryo electron microscopes

General understanding of single-particle cryo-EM workflow, experience in cryo-electron tomography,
Cs correction and Phase contrast systems would be added benefits.

Proven organizational and time management skills to successfully set priorities, meet established
deadlines and recognize new problems with constantly changing priorities and frequent interruptions.

Strong communication skills (both verbal and written) needed to interact professionally and
effectively in the work environment. Ability to read, comprehend, and discuss research materials.
Proven ability to write, edit and proofread research results. Skill at explaining difficult concepts
and training users and students.

Ability to diplomatically and professionally interact with all levels of University faculty and
staff and external contacts.

Proven ability to perform minor maintenance and alignment of electron microscopes.


APPLICATION INSTRUCTIONS

Please go to: jobs.wustl.edu and search for job ID 36721
Research Specialist (Cryo-Electron Microscopy) - Center for Cellular Imaging

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From: microscopy.listserver-at-gmail.com
Date: Fri, 26 May 2017 07:53:33 -0500
Subject: [Microscopy] viaWWW:Pemtron Pemscan SEM technical

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Email: steve.sem-at-icloud.com Name: Steve Perry

Organization: SEM Lab support

Title-Subject: [Filtered] Pemtron Pemscan SEM technical
Message: I'm looking for any service information for the Pemscan PS-230 SEM. Anything like the
engineer login, schematics or parts diagrams would be helpful. This SEM looks and acts like an ISI
sold under a new name.

Thanks in Advance,

Steve Perry

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From: microscopy.listserver-at-gmail.com
Date: Sat, 27 May 2017 12:58:09 -0500
Subject: [Microscopy] viaWWW:Job Opening - TEM Specialist

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X-from: stefano-at-soquelec.com

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Email: stefano-at-soquelec.com Name: Stefano Rubino

Organization: Soquelec Limited

Title-Subject: [Filtered] Job opportunity as electron microscopy sales and service representative

Message: Start date: ASAP
Hourly/yearly wage: To be discussed
Job type: Permanent
Relocation/travel requirements: up to 80% travelling
Soquelec Limited, founded in 1974, is an established distributor of high-end imaging analysis
equipment, sample preparation equipment, and consumables for life and material science laboratories
across Canada.
Job Description We are looking for an industrious and self-driven sales and service representative
to represent our company in Ontario. You will be responsible for the sales and service of scientific
equipment from a variety of suppliers (TESCAN, Gatan, Bruker, Quorum, etc.), as well as establish
and maintain strong business relationships with our customers. You will be working with the rest of
the sales team and the administrative staff closely to achieve your sales goals. The ideal candidate
should have at least a Bachelor’s Degree in Science, Engineering or related field, a driver’s
license and a passport for international travel. You will also need to have excellent communication
skills in English; a working knowledge of French would be preferred. The position entails frequent
travels to our customers’ locations in Canada and to supplier meetings and training sessions in the
USA or Europe.
Key Responsibilities
• Initializing contacts with potential customers and qualifying sales opportunities
• On-site presentations and/or demonstrations of scientific equipment
• Following up and closing sales opportunities in a timely manner
• Installation and/or servicing instruments
• Training end users of said instruments
• Updating CRM according to the sales pipeline
• Participating in applicable trade shows and conferences
Requirements
• Bachelor’s Degree in Science or Engineering
• Excellent communication skills in English
• Driver’s license
• Willing and ready to travel frequently
• Ability to identify customers’ needs and follow up accordingly
• Self-motivation and enthusiasm
• Team player
Assets
• Experience in electron microscopy or x-ray imaging
• Previous sales/retail/customer service experience
• Familiarity with Microsoft Dynamics CRM
• Working knowledge of French


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Email: mibuckett-at-mmm.com Name: Mary Buckett

Organization: 3M Co.

Title-Subject: [Filtered] Job Opening - TEM Specialist

Message: 3M is seeking a TEM Research Specialist for the Corporate Research Analytical Laboratory
(CRAL) located in Maplewood, MN.
Job Summary:
The person hired for the position of Research Specialist (Transmission Electron Microscopy) will
partner with corporate laboratories as well as division R&D and manufacturing in the development of
technologies and products.

Preferred Qualifications:
Ph.D. in Materials Science, Polymer Science, Chemistry, Chemical Engineering, Physics or related
discipline from an accredited university.

More information and job application can be found at:
https://3m.wd1.myworkdayjobs.com/en-US/Search/job/US-Minnesota-Maplewood/Research-Specialist-Transmission-Electron-Microscopy---Maplewood--MN-_R00031146-1

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From: microscopy.listserver-at-gmail.com
Date: Sat, 27 May 2017 12:59:03 -0500
Subject: [Microscopy] viaWWW:Cryo SEM Workshop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

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Email: stacie-at-ems-secure.com Name: Stacie Kirsch

Organization: Electron Microscopy Sciences

Title-Subject: [Filtered] Cryo SEM Workshop

Message: EMS Microscopy Academy announces the Cryo SEM Workshop in July. This course will cover the
process of rapid freezing, fracturing, coating and imaging of a variety of samples. For more
information, see http://ow.ly/WzPe30bmk8v

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From: microscopy.listserver-at-gmail.com
Date: Sat, 27 May 2017 13:02:57 -0500
Subject: [Microscopy] viaWWW:Job opening: Senior Research Associate - Electron Microscopy

Contents Retrieved from Microscopy Listserver Archives
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X-from: YaqiaoWu-at-boisestate.edu

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Email: YaqiaoWu-at-boisestate.edu Name: Yaqiao Wu

Organization: Boise State University/Center for Advanced Energy Studies

Title-Subject: [Filtered] Job opening: Senior Research Associate - Electron Microscopy

Message: Dear colleagues,

Micron School of Materials, Science and Engineering at Boise State University is looking for
candidates for the position of Senior Research Associate in electron microscopy.

To apply online, please go to
https://boisestate.taleo.net/careersection/ex/jobdetail.ftl?job=170442&tz=GMT-06:00

Job description details:

Position Overview

Boise State University, powered by creativity and innovation, stands uniquely positioned in the
Northwest as a metropolitan research university of distinction. The Center for Advanced Energy
Studies (CAES) seeks candidates for the position of Senior Research Associate.

This position works in a multi-collaborative environment at the Center for Advanced Energy Studies
in Idaho Falls, Idaho, and assists Boise State University, Idaho State University, University of
Idaho, University of Wyoming, Idaho National Laboratories, and external customers with conducting
research in both the Microstructure and Characterization Suite (MaCS) and Advanced Materials
Laboratory (AML). Responsibilities include sample preparation, microstructure characterization and
chemical analysis utilizing Focused-Ion Beam (FIB), Scanning Electron Microscopy (SEM) and
Transmission Electron Microscopy (TEM), involvement in materials research projects; handle
radioactive materials.

You will have the opportunity to: • Assist with sample preparation on the bench top and inside of a
glovebox using traditional cutting and polishing methods as well as advanced techniques including
FIB, ion milling and coating deposition; • Perform characterization of materials including electron
microscopy (FIB/SEM, TEM) and chemical analysis; train new users on the equipment as needed;
• Operate equipment and conduct experiments in the Advanced Materials Laboratory including spark
plasma sintering, heat treatments, and mechanical testing; • Interact with customers, assessing
problems, and assisting in solving materials related problems;
• Safely handle, store, and dispose of radioactive materials on a daily basis; • Maintain compliance
with safety, security, and environmental regulations on the safe handling of chemicals and
radioactive materials; • Trouble shoot issues and coordinate the repair of equipment/tools as needed.
Qualifications
At a minimum you should have: A Bachelor’s degree in materials science or related discipline and 8
years of experience in a similar type of work that includes the following:
• An understanding of energy materials and high temperature processing equipment;
• Experience in sample preparation techniques;
• Experience in electron microscopy techniques;
• Ability to translate, adapt and apply academic and/or practical knowledge to engineering research;
• Ability to instruct others on instrument use, scientific principles, and safety;
• Excellent oral and written communication skills;
• Ability to work with a diverse group of people; Knowledge of good safety practices.
Preferentially, you will have: Master’s degree in materials science or related discipline, with a
minimum of 6 years of experience in a materials research laboratory that includes the following:
• Experience in safe handling and managing of radioactive materials;
• Experience in working in a glovebox environment;
• Experience in advanced characterization/chemical analysis techniques including FIB/SEM, TEM, EBSD
and EDS;
• Proficiency in the maintenance and use of advanced materials processing techniques.

Salary and benefits: Salary is commensurate with experience. An excellent benefits package is
available for eligible employees, for more information visit:
http://hrs.boisestate.edu/careers/benefits/.
If you are interested in this position: Please apply online and upload a cover letter and resume
(including dates of employment) and the names of three professional references with contact
information.
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From: microscopy.listserver-at-gmail.com
Date: Tue, 30 May 2017 19:55:01 -0500
Subject: [Microscopy] viaWWW:Hitachi S4700: air sensitive sample

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Email: pscallio-at-dal.ca Name: Pat Scallion

Organization: Dalhousie University

Title-Subject: [Filtered] Hitachi S4700: air sensitive sample

Message: Hi,
I have a user with a sample that must be kept under an Argon atmosphere until placed in the SEM
exchange chamber, and I have never had to deal with this situation before.I am wondering if the
following procedure will work, or if there are any issues with it.

Our SEM has a separate exchange chamber prior to the Sample chamber. We propose to tape a flexible
glove bag around the frame of the SEC, containing the samples and mounts. We would first evacuate
the bag using the rotary pumps, then seal the exchange chamber. Then, we will flush the glove bag
several times with argon using the glove bag ports only.

Once the argon atmosphere is present, the samples will be removed from their carrier and placed on
the SEM mount in the glove bag, then into the exchange chamber and evacuated. Once vacuum is reached
the sample will be put into the sample chamber. Hopefully all the argon will be gone by then.

After viewing, there is no current plan to try and keep the samples from the atmosphere.

Also, our SEM is used by over 90 researchers from several faculties. Would a temporary set-up like
this with the argon cause any problems or concerns down the road?

As I said, this is the first time for me trying something like this, and I don't want any surprises.

Thanks in advance for your help!

Pat Scallion


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From: microscopy.listserver-at-gmail.com
Date: Tue, 30 May 2017 22:19:48 -0500
Subject: [Microscopy] viaWWW:Hitachi S4700: air sensitive sample

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Email: miketoalson-at-gmail.com Name: Mike Toalson

Organization: NanoImages, LLC

Title-Subject: [Filtered] viaWWW:Hitachi S4700: air sensitive sample

Message: Hello Pat,

Your concept sounds feasible but I'll let the experts on here comment further.

I thought you might want to have a look at 2 other solutions that I have seen:

1) VacuShut sample transfer device - this was sold by Agar Scientific but I don't see it on their
website now. It was invented at Karlsruhe Inst for Tech. Here is a flyer PDF showing how it works.
Maybe the inventors listed on this know where you can acquire it now:
http://www.int.kit.edu/downloads/INT_Research/Flyervacushut.pdf
2) Transfer Module from Kammrath Weiss - a more elegant solution (and probably more expensive)
https://www.kammrath-weiss.com/en/products/materials/transfer-module.html
Good Luck!!

Mike Toalson
NanoImages, LLC
t • 866.601.6266 m • 530.691.3180
e • mtoalson-at-nanoimages.com
web • http://www.nanoimages.com/
skype • mike.toalson


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Email: mailto:pscallio-at-dal.ca Name: Pat Scallion

Organization: Dalhousie University

Title-Subject: [Filtered] Hitachi S4700: air sensitive sample

Message: Hi,
I have a user with a sample that must be kept under an Argon atmosphere until placed in the SEM
exchange chamber, and I have never had to deal with this situation before.I am wondering if the
following procedure will work, or if there are any issues with it.

Our SEM has a separate exchange chamber prior to the Sample chamber. We propose to tape a flexible
glove bag around the frame of the SEC, containing the samples and mounts. We would first evacuate
the bag using the rotary pumps, then seal the exchange chamber. Then, we will flush the glove bag
several times with argon using the glove bag ports only.

Once the argon atmosphere is present, the samples will be removed from their carrier and placed on
the SEM mount in the glove bag, then into the exchange chamber and evacuated. Once vacuum is reached
the sample will be put into the sample chamber. Hopefully all the argon will be gone by then.

After viewing, there is no current plan to try and keep the samples from the atmosphere.

Also, our SEM is used by over 90 researchers from several faculties. Would a temporary set-up like
this with the argon cause any problems or concerns down the road?

As I said, this is the first time for me trying something like this, and I don't want any surprises.

Thanks in advance for your help!

Pat Scallion


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From: roseann.csencsits-at-schafercorp.com
Date: Wed, 31 May 2017 07:05:42 -0500
Subject: [Microscopy] RE: viaWWW:Hitachi S4700: air sensitive sample

Contents Retrieved from Microscopy Listserver Archives
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Hi Pat,

It sounds like something I did back in 2002 on a similar SEM back at the Electron Microscopy Center at Argonne National Lab. Should work just fine and an inert gas like argon will not cause any problems. Science and research are all about having fun and trying new things.

Enjoy!
Roseann

Roseann Csencsits
President Northern California Society for Microscopy
Group Leader-Microbeam Analyses
Schafer Vallecitos Laboratories, Inc.
a Belcan company
6705 Vallecitos Rd.
Sunol, CA 94586
925-862-4345
roseann.csencsits-at-schafercorp.com


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Email: pscallio-at-dal.ca Name: Pat Scallion

Organization: Dalhousie University

Title-Subject: [Filtered] Hitachi S4700: air sensitive sample

Message: Hi,
I have a user with a sample that must be kept under an Argon atmosphere until placed in the SEM exchange chamber, and I have never had to deal with this situation before.I am wondering if the following procedure will work, or if there are any issues with it.

Our SEM has a separate exchange chamber prior to the Sample chamber. We propose to tape a flexible glove bag around the frame of the SEC, containing the samples and mounts. We would first evacuate the bag using the rotary pumps, then seal the exchange chamber. Then, we will flush the glove bag several times with argon using the glove bag ports only.

Once the argon atmosphere is present, the samples will be removed from their carrier and placed on the SEM mount in the glove bag, then into the exchange chamber and evacuated. Once vacuum is reached the sample will be put into the sample chamber. Hopefully all the argon will be gone by then.

After viewing, there is no current plan to try and keep the samples from the atmosphere.

Also, our SEM is used by over 90 researchers from several faculties. Would a temporary set-up like this with the argon cause any problems or concerns down the road?

As I said, this is the first time for me trying something like this, and I don't want any surprises.

Thanks in advance for your help!

Pat Scallion


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From: nmz787-at-gmail.com
Date: Thu, 1 Jun 2017 12:45:17 -0500
Subject: [Microscopy] Typical metallic purity for FIB source?

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Mississippi State is seeking a Research Associate - Microscopy

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From whitejoye45-at-gmail.com Wed May 31 18:53:17 2017
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I'll have to replace my LMIS soon and was just wondering what they
start as. Seems like the less pure, the less ideal your focused beam
spot would become, more 'chromatic' aberration probably.
Just looking around I find 99.99% pure through 99.99995% available...
so where would these be relative to what precision FIB would commonly
use?

I'd guess that for some applications, a custom ion mill could tolerate
a wide range of purity (not that I can imagine one at the moment). It
leads to the question of, do all impurities ionize, or would some
remain as 'slag' that prevents transport (flow) of clean metal to the
emission point.

--
-Nathan

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From: Kyoung.Jo-at-rockets.utoledo.edu
Date: Mon, 5 Jun 2017 17:07:51 -0500
Subject: [Microscopy] TEM need help on staining methods

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Hello Nathan,

When I was at Drexel, I remember the FIB scientist discovered (with
the help of another scientist at Applied Beams) a solution as to why
all of our ion beam images and milling patterns had these ghostly
shadows present. For example, if you tried to drill a very small hole
into a silicon nitride membrane, instead of single hole you would get
two! This turned out to be because our ion source had two different
isotopes of gallium present, and each isotope experienced a slightly
different path through the optics and was focused to a crossover that
was slightly laterally displaced with respect to each other. The
purity of the source is certainly one thing to consider but apparently
the isotopic distribution of even a pure gallium source is another.
This doesn't answer your question directly but I thought you might
find it interesting. I imagine the FIB source suppliers go to some
extra lengths the ion source is isotopically pure Ga-69...

Cheers,
Chris

On Thu, Jun 1, 2017 at 1:55 PM, {nmz787-at-gmail.com} wrote:
}
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} I'll have to replace my LMIS soon and was just wondering what they
} start as. Seems like the less pure, the less ideal your focused beam
} spot would become, more 'chromatic' aberration probably.
} Just looking around I find 99.99% pure through 99.99995% available...
} so where would these be relative to what precision FIB would commonly
} use?
}
} I'd guess that for some applications, a custom ion mill could tolerate
} a wide range of purity (not that I can imagine one at the moment). It
} leads to the question of, do all impurities ionize, or would some
} remain as 'slag' that prevents transport (flow) of clean metal to the
} emission point.
}
} --
} -Nathan
}
} ==============================Original Headers==============================
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From bjzhengqs-at-ruihesoft.com Thu Jun 1 22:00:57 2017
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Reply-To: {sarahadams212-at-yandex.com}

Dear colleagues,

We would like to remind you the Yale Microscopy Workshop which will be held on June 6 & 7, 2017.

We invite you to participate in a two day workshop. This free annual event is a combination of symposium, demonstrations, technical lectures and small group practicals. It is open to the international research community and only requires your free registration.

Time: June 6 - 7th, 2017
Location: Yale University School of Medicine, The Anlyan Center (TAC), New Haven, CT
Cost: Free on-line or on-site registration
Information: https://medicine.yale.edu/lab/microscopy.

Features:
- Symposia on image analysis and high-dimensional imaging
- Concurrent workshop on correlative live-cell STED and cryo-electron microscopy, and cryo EM techniques
- Demonstrations, technical lectures, and small group practicals.

Please see the website for free registration, list of accessible equipment, and the schedule of events.

We look forward to seeing you there,

The organizers,
Ann Haberman ann.haberman-at-yale.edu {mailto:ann.haberman-at-yale.edu}
Derek Toomre derek.toomre-at-yale.edu {mailto:derek.toomre-at-yale.edu}
Joerg Bewersdorf joerg.bewersdorf-at-yale.edu {mailto:joerg.bewersdorf-at-yale.edu}
Xinran Liu xinran.liu-at-yale.edu {mailto:xinran.liu-at-yale.edu}




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From mail.3185.educanet2.ch-at-smtp.bestone.cz Fri Jun 2 15:43:35 2017
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Hello,

I am trying to figure out what part of the cellular organelle or molecules, the ethanolic phosphotungstic
acid (E-PTA) binds to show the electron dense staining? I have found that its commonly used for visualizing phospholipids or nerve ending. However, I dont know if E-PTA binds to lipids molecules or membranes. Does anyone know exactly what E-PTA binds to?

Thank you!

Sincerely,

Kyoung Jo


==============================Original Headers==============================
6, 48 -- From Kyoung.Jo-at-rockets.utoledo.edu Mon Jun 5 17:07:51 2017
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Date: Tue, 6 Jun 2017 20:01:11 -0500
Subject: [Microscopy] viaWWW:Ultracut E or S control box

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From: zaluzec-at-microscopy.com
Date: Tue, 6 Jun 2017 20:02:14 -0500
Subject: [Microscopy] viaWWW:FEI single tilt holder contamination

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Email: lavoie-at-uw.edu Name: Ellen Lavoie

Organization: UW - MAF

Title-Subject: [Filtered] FEI single tilt holder

Message: Hello all,

I'm trying to narrow down some odd contamination on our FEI ST holder
but am posing the the following question:

Exactly what elements should show up in a case of a pristine new ST
holder? Obviously, yes, I know there is naturally some contamination
and variation with wear and age. Have not contacted tech at FEI yet
because I thought surely someone out here in microscopy land has tested
this??

Cheers,
Ellen
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From: wij.muss-at-aon.at
Date: Tue, 6 Jun 2017 20:03:42 -0500
Subject: [Microscopy] Re: TEM need help on staining methods

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Dear Kyoung Jo,

I guess you are referring to the Ethanolic Phosphotungstic Acid / Ethanolic
PTA (E-PTA) staining technique which was introduced by Gray (1959)
[cf: Stains and Cytochemical Methods, M.A. Hayat(ed) 1993] Ethanolic
Phosphotungstic Acid, Chapter 8, p 284, where you can find a collection of
stained and unstained material/tissue components, predominantly of/regarding
nerve synapse(s)

Perhaps you can find some or more of the information you are looking for:
in:
Assembly of Proteins to Postsynaptic Densities after Transient Cerebral
Ischemia
Bing-Ren Hu, Minkyu Park, Maryann E. Martone, Wolfgang H. Fischer, Mark H.
Ellisman, and Justin A. Zivin
In: J. Neurosci., January 15, 1998, 18(2):625633
X-from: Materials and Methods
Electron microscopic studies
Tissue sections from experimental and control animals were stained with 1%
ethanolic phosphotungstic acid (E-PTA) by the method of Bloom and Aghajanian
(1966, 1968). Coronal brain sections were cut to a thickness of 200 mm
with a Vibratome through the level of the dorsal hippocampus and post-fixed
for 1 hr with 4% glutaraldehyde in 0.1 M cacodylate buffer, pH 7.4. Sections
then were dehydrated in an ascending series of ethanol to 100% and stained
for 1 hr with 1% phosphotungstic acid prepared by dissolving 0.1 gm of PTA
in 10 ml of 100 ethanol and then adding four drops of 95% ethanol from a
Pasteur pipette. Sections were embedded in Durcopan ACM. Isolated PSDs were
fixed with 4% glutaraldehyde in 0.1
M cacodylate buffer, pH 7.4, osmicated for 60 min in 1% osmium tetroxide,
stained en bloc in 1% aqueous uranyl acetate, dehydrated, and embedded in
Durcopan ACM. Ultrathin and semithin (1 mm) sections of parietal cortex
(layers IIV) were cut and evaluated with a JEOL 100CX electron microscope
or a JEOL 4000EX intermediate high-voltage electron microscope without
additional staining. Sections of isolated PSDs were counterstained with lead
citrate before examination in the electron
microscope. [not knowing whether your methodological approach is similar to
the described one in the study]:
"The E-PTA method selectively stains the postsynaptic density, the
presynaptic grid, and material in the synaptic cleft but leaves most other
structures less stained (Bloom and Aghajanian, 1966, 1968)."
X-from References:
Bloom FE, Aghajanian GK (1966) Cytochemistry of synapses: a selective
staining method for electron microscopy. Science 154:15751577.
Bloom FE, Aghajanian GK (1968) Fine structural and cytochemical analysis of
staining of synaptic junctions with phosphotungstic acid.
J Ultrastruct Res 22:361375.


Also, you can find hints on the nature of material which is stained (or less
or not stained) by E-PTA in:
R A Horowitz and C L Woodcock: Alternative staining methods for Lowicryl
sections
J Histochem Cytochem 1992 40: 123; DOI: 10.1177/40.1.1370308,
-at-http://jhc.sagepub.com/content/40/1/123

Perhaps a further reading:
OBSERVATIONS ON THE KINETICS OF URANYL ACETATE AND PHOSPHOTUNGSTIC ACID
STAINING OF CHROMATIN IN THIN SECTIONS FOR ELECTRON MICROSCOPY
P. A. CATTINI and H. G. DAVIES
STAIN TECHNOLOGY Vol. 59. No. 5 pp291-304 Copyright(C) 1984 by The
Williams & Wilkins Co.

Many articles more can be found Googleing for | e-PTA OR "ethanolic PTA"
specific* staining |
Googleing | e-PTA OR "ethanolic PTA" binding specific* staining | reduces
the number of results and finds some rapid info about:
{ Ethanolic phosphotungstic acid staining of glutaraldehyde fixed cells
specifically contrasts basic proteins, and reveals structural details that
are not easily discerned in uranyl acetate stained or osmicated sections
(Bloom and Aghajanian, 1966; 1968).
.... procyclic T. brucei cell stained in this manner. The nucleus and
kinetoplast are strongly contrasted and the cytoplasm shows a granular
structure of intermediate contrast. The mitochondrial matrix is not
contrasted. ......
Strong binding of E-PTA to basic residues of histone proteins (Sheridan and
Barrnett, 1969) accounts for the strong contrast observed in the nucleus.
Lysine and arginine residues of proteins are believed to be the binding
sites for the PTA, a suggestion supported by the finding that acetylation
reduced PTA binding to isolated histones (Sheridan and Barrnett, 1969).
Cited from:
Structural asymmetry and discrete nucleic acid subdomains in the Trypanosoma
brucei kinetoplast
GLUENZ et al, 2007: Mol Microbiol. 2007 Jun 1; 64(6): 15291539.
doi: 10.1111/j.1365-2958.2007.05749.x; PMCID: PMC1974780; cf.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1974780/

Hope this helps anyway in a first attempt,

Best wishes and regards
Wolfgang MUSS, PhD
Retired MSA-Member
SALZBURG, AUSTRIA


-----Ursprngliche Nachricht-----
Von: Kyoung.Jo-at-rockets.utoledo.edu [mailto:Kyoung.Jo-at-rockets.utoledo.edu]
Gesendet: Dienstag, 6. Juni 2017 00:28
An: wij.muss-at-aon.at
Betreff: [Microscopy] TEM need help on staining methods
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From: Philip.Koeck-at-ki.se
Date: Wed, 7 Jun 2017 03:11:33 -0500
Subject: [Microscopy] [TEM] CTF and SNR

Contents Retrieved from Microscopy Listserver Archives
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Hi.

I've recently encountered a bit of a conundrum:

I'll assume most of the noise in an image of an ice-embedded bio-molecule is due to variations in the ice, so called structural noise.
The CTF describes the contrast transfer for both signal and noise in the same way.
So, what is the point of changing the CTF by defocusing and/or using a phase plate.
The SNR should be unchanged.

I can think of one explanation: If the signal spectrum is very different from the noise spectrum one could chose a CTF that enhances the
resolution bands where the difference is big.

Else: Is there some other factor that affects visibility of the molecule than SNR?

All the best,

Philip


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From: microscopy.listserver-at-gmail.com
Date: Mon, 12 Jun 2017 18:08:52 -0500
Subject: [Microscopy] viaWWW: Microscopy Research Associate position

Contents Retrieved from Microscopy Listserver Archives
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Dear All,
there are news from Wuerzburg and the nanoflight system:
I finally bought a very nice new FE-SEM, a MIRA3 from TESCAN.

Have a look at some trials:

T-Cells:
https://vimeo.com/216668452

This is the first extra-small field-of-view test of my new MIRA3 FE-SEM on some T-cells supplied by Bruce Levine, Purdue University.
The single cell is ca. 12 micron wide, the round iron beads five micron.
In my opinion this is the first complete 360 curve ever "flown" actually around a T-cell in the scanning electron microscope.
Field-of-view at the end of the second sequence is ca. 45 micron wide.
These sequences had been a first test for the accuracy of both the SEM electronics and the SmarAct 8 axes piezostage. Both came
out very good.

Think about the accuracy needed to succeed twice: first more than 30 waypoints for the sequences are set up, then overlooked at a
"pre-flight", then going back to the beginning and starting sending out the interpolated frame positions, more than 500... I ended
up in a voxel of ca. 5x5x5 micron inaccuracy.

Here is a second nanoflight on a very small 3d printed elephant (150x150x80 micron size):
https://vimeo.com/216811056

I hope that in a few weeks I will be able to do 3D nanoflights in SEM down to cell size.

I am looking for interesting specimen - preferably single cells on glass - to be imaged as nanoflights or to be reconstructed as
3d models with SFM software.

Anybody willing to join the project: feel free to contact me.



Best wishes,
Stefan



--


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Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
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++49-175-7177051 Mobile

Websites:
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www.stefan-diller.com
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Anfahrt: http://Mail.map24.com/Stefan.Diller
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Email: mryan-at-cshl.edu Name: Marjorie Ryan

Organization: Cold Spring Harbor Laboratory

Title-Subject: [Filtered] Microscopy Research Associate position.

Message:
Thank you very much for your assistance.

Sincerely,

Marjorie Ryan
Cold Spring Harbor Laboratory
mryan-at-cshl.edu

Research Associate – Electron Microscopy Technologist

Cold Spring Harbor Laboratory seeks a highly motivated dedicated individual to work in a
state-of-the-art Microscopy Shared Resource.

The individual should have extensive practical expertise in biological sample preparation for
transmission and scanning electron microscopy. Hands-on knowledge of confocal and widefield
fluorescence microscopy would also be a plus. The candidate will help users design innovative
experiments and they will carry out sample preparation and imaging as well as assist in data
interpretation.

Excellent verbal and written communication skills, ability to work with multiple users in a
supporting role, and ability to work independently and proactively with limited supervision are
essential. A Bachelor’s degree in biology or related discipline is required. One to three years of
experience working in a Microscopy Shared Resource is preferred.

How to Apply:

Email: mryan-at-cshl.edu
Interested individuals should also apply for this position via the CSHL careers website at
https://cshl.peopleadmin.com/postings/11688

Position Number 01779-R

Applicants should include a resume along with a description of their practical expertise and the
names as well as email addresses of 3 references.

Cold Spring Harbor Laboratory is a world-renowned research and educational institution recognized
internationally for its excellence in ground-breaking research programs in cancer, neuroscience,
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From: microscopy.listserver-at-gmail.com
Date: Mon, 12 Jun 2017 18:09:30 -0500
Subject: [Microscopy] viaWWW: X-Ray Microanalysis Workshop

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Email: stacie-at-ems-secure.com Name: Stacie Kirsch

Organization: Electron Microscopy Sciences

Title-Subject: [Filtered] X-Ray Microanalysis Workshop

Message: EMS Microscopy Academy announces our X-Ray Microanalysis Workshop: A Complete Picture. This
course covers qualitative and semi quantitative analysis beginning with the generation of background
and characteristic of x-rays, nomenclature, and peak family ratios.

September 13 - 15, 2017
8:00 a.m. - 4:30 p.m.
Hatfield, Pennsylvania, USA

For more information, visit http://ow.ly/Zb9F30cmyuf



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From: microscopy.listserver-at-gmail.com
Date: Tue, 13 Jun 2017 07:59:53 -0500
Subject: [Microscopy] viaWWW:Job opening at Stanford: Life Science Technician II

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Email: kmicheva-at-stanford.edu Name: Kristina Micheva

Organization: Stanford University School of Medicine

Title-Subject: [Filtered] Job opening at Stanford: Life Science Technician II

Message: Job Description
Life Science Technician II - 75238
Description
The Department of Molecular and Cellular Physiology is seeking a Life Science Technician II to
assist with preparation of laboratory biological tissue specimens for Array Tomography, and for
general laboratory maintenance and organization.

Duties include preparation of specimens and reagents for immunostaining, light and electron
microscopy, tissue thin and ultra-thin sectioning, handling of experimental animals (rats and mice),
including whisker trimming and perfusion, fluorescent image acquisition, and analysis of volumetric
image data, basic statistical analysis, experimental record-keeping, general laboratory compliance
and record-keeping tasks, and general laboratory maintenance and organization.

Applicants already having experience in desired skills are preferred, but training in specific
procedures will be provided.
Qualifications
Experience in the skills/duties listed above is preferred, but aptitude is required and training
will be provided. Training, if necessary, in general laboratory tasks will be provided.

In addition:
2+ year of relevant laboratory experience or relevant college coursework.
A strong interest in cellular neuroscience.
Meticulous attention to detail and record keeping in laboratory procedures.
Excellent manual dexterity.
Preferred: Experience working in a biology laboratory.
Preferred: Ultrathin sectioning.
Preferred: Understanding of basic principles of light microscopy.
Preferred: Experience with animal handling (rodents) and perfusion fixation. Ability to work
independently and within a collaborative environment.
Proficiency with Microsoft Office software (Word, Excel, PowerPoint).
Aptitude for learning and use of scientific software (ImageJ).
Job : Research
Location : School of Medicine
Schedule : Full-time
Grade: A24
Job Code: 5644

Please apply at http://stanfordcareers.stanford.edu/job-search
Job #75238

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From: hsia627-at-hotmail.com
Date: Tue, 13 Jun 2017 19:01:10 -0500
Subject: [Microscopy] Course announcement: Biological EM Sample Processing 07/13-14, 2017

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues,

We are pleased to announce that the Electron Microscopy Core Imaging Facility (EMCIF) at the University of Maryland Baltimore will be offering a Biological EM Sample Processing mini-course on July 13th and 14th, 2017 http://www.dental.umaryland.edu/UMBEMProcessingCourse/ .

The course is designed to teach any individual who wishes to learn biological sample processing for electron microscopy. No experience is required. The course will be limited to six participants.

--Course format: lectures, demonstrations, and hands-on practice.

--Topics: Chemical fixation, cryo-immobilization, resin embedding, conventional and modern rapid processing methods, microwave assisted processing, automated sample processing, SEM biological sample processing and Negative staining of particulate specimen.

--Instrument Demonstration: high pressure freezer, automated freeze substitution, critical point dryer and automated EM sample processing

--Other related courses: EMCIF also offers room temperature ultramicrotomy and immunogold labeling mini courses in September and October, this year. The minicourses follow a similar format covering principles, practices, and troubleshooting with an emphasis on hands-on practice and instrument demonstration.

--More information: Email coreimaging-at-umaryland.edu or visit our website
http://www.dental.umaryland.edu/core-imaging/workshops-and-courses/

Thanks.

Sincerely,

Ru-ching Hsia, Ph.D.
Associate Professor and Director
Electron Microscopy Core Imaging Facility
University of Maryland, Baltimore
Rm 696B, Howard Hall, 660 W. Redwood St. Baltimore, MD 21201


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11, 59 -- From: Ruching hsia {hsia627-at-hotmail.com}
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11, 59 -- Subject: Course announcement: Biological EM Sample Processing 07/13-14, 2017
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11, 59 -- 2017
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From: microscopy.listserver-at-gmail.com
Date: Tue, 13 Jun 2017 19:56:14 -0500
Subject: [Microscopy] viaWWW:TEM - Camera-head eletronics for Gatan model 794

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Email: eduardo.isoppo-at-ufsc.br Name: EDUARDO ISOPPO

Organization: Federal University of Santa Catarina

Title-Subject: [Filtered] TEM - Camera-head eletronics for Gatan model 794

Message: Hello All,
My name is Eduardo and Im working in a electron microscopy facility in Florianopolis -
Brazil. Our CCD camera (Gatan 794) installed in the TEM (Jeol 2100) is down due problems in the
camera-head eletronics. The
circuit has to be replaced. Gatan (By Jeol - Brazil) said that this model is no longer
available for maintenance service (it is too old). Does anyone here knows where I can get support
for this camera or where I can find parts for this camera?
thanks

--
Eduardo de Almeida Isoppo, Ph.D
Physicist Federal University of Santa Catarina - UFSC Florianpolis/SC - Brazil

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From: microscopy.listserver-at-gmail.com
Date: Tue, 13 Jun 2017 19:56:54 -0500
Subject: [Microscopy] viaWWW:Job Opening: Summer Internship at Gatan

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Email: apakzad-at-gatan.com Name: Ana Pakzad

Organization: Gatan Inc.

Title-Subject: [Filtered] Job Opening: Summer Internship at Gatan
Message: We are seeking candidates for a summer internship position at Gatan, CA office. This role
will support our R&D team by conducting electron microscopy on various types of materials, analysis
of results and preparing reports. For more details please go to:
http://www.gatan.com/company/careers/summer-internship-imaging


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From: microscopy.listserver-at-gmail.com
Date: Tue, 13 Jun 2017 19:57:47 -0500
Subject: [Microscopy] viaWWW: TEM/Cryo-TEM: Research Scientist position available

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Email: vitha-at-tamu.edu Name: Stanislav Vitha

Organization: Texas A&M University

Title-Subject: [Filtered] TEM/Cryo-TEM: Research Scientist position available

Message: The Microscopy and Imaging Center (MIC) at Texas A&M University is seeking to hire a staff
research scientist. The new hire will provide service and user training in TEM/cryo-TEM imaging,
sample preparation, and in situ elemental/molecular analyses of biological and soft matter material
science samples.
Required Education and Experience: A doctoral degree in a life science related field plus six years
of experience in electron microscopy.

For additional details, please see http://microscopy.tamu.edu/whats-new
Instructions for Applicants: Please submit a resume/CV and cover letter to joeashworth-at-tamu edu to
apply.





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From: eikonika-at-otenet.gr
Date: Wed, 14 Jun 2017 05:01:16 -0500
Subject: [Microscopy] removing the stage from a jeol JSM5600LV

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Hi
We are trying to pull out the stage of our JSM5600LV machine and separate it
completely from the chamber. Can anybody help us to tell what screws we
have to remove?
Thanks in advance for your time
yorgos

Dr Yorgos Nikas
Athens Innovative Microscopy
Skra 36 Voula 16673 GREECE

Tel/fax +30 210 8957677
mobile +30 6945 107477
www.eikonika.netwww.aim.cat
*************************************





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6, 20 -- Subject: removing the stage from a jeol JSM5600LV
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From: michal.jarnik-at-nih.gov
Date: Wed, 14 Jun 2017 14:02:24 -0500
Subject: [Microscopy] Osmicating cryosections

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Dear listers,

Does anyone have experience with post-fixing Tokuyasu cryosections with osmium tetroxide? The purpose would be to gain better and more “standard” membrane contrast. I tried to fix the grids on a drop of 2% OsO4, but there was very little (if any) change. I am sure people tried before, but do not remember reading about it. Maybe OTO enhancement or...?

Thanks for ideas,

Michal Jarnik
NIH






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From: microscopy.listserver-at-gmail.com
Date: Wed, 14 Jun 2017 17:44:42 -0500
Subject: [Microscopy] viaWWW:Hitachi S800 Manual and Service manual Request

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Email: lbochtler-at-protonmail.ch Name: Lukas B.

Title-Subject: [Filtered] Hitachi S800 Manual and Service manual Request

Message: Hello,

I just scored a Hitachi S800 electron microscope, sadly most cables between the main console (with
the electron column) and the control desk have been cut. I can replace most if not all cables, so
that isn't much of a problem.

However, i did not get any documentation with it, since i don't know if the original owner will ever
manage to find any documentation for it, i decided to ask you guys.

Ideally i would need both the user and service manual for the microscope, possibly with the computer
software (since im not sure if i can get the computer for it as well, since the microscope and the
other parts are now in 2 different locations)

Ideally id also need the power requirements for the microscope, what voltage dose it need, how much
power dose it draw, and how many power phases dose it need.


Best regards,

Lukas B.

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From: microscopy.listserver-at-gmail.com
Date: Fri, 16 Jun 2017 07:56:45 -0500
Subject: [Microscopy] viaWWW:light elements measurement in metals

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Email: M.Simonelli-at-nottingham.ac.uk Name: Marco Simonelli

Organization: University of Nottingham

Title-Subject: [Filtered] light elements measurement in metals

Message: Dear Fellow Microscopists,

I am looking for an advice on a reliable technique to measure the concentration of light elements
(an in particular C) in metal/alloys. I would be very grateful if you can share with me your
experience and comments.


Many thanks,
Regards,
Marco

Dr Marco Simonelli
Centre for Additive Manufacturing
The University of Nottingham
University Park
Nottingham, UK



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From: wij.muss-at-aon.at
Date: Mon, 19 Jun 2017 06:49:48 -0500
Subject: [Microscopy] just to be sure about replies from MSA-Listserver....and Season's Greetings / good wishes for the summertime

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Dear all,
dear Michal,

I just wondering about nobody seems to have answered to your interesting question until now (but it could be that you, dear Michal, have gotten some personal replies due to so many unnecessary OOO-[NOT ‚OTO‘- (☺) ]messages from the list itself).

If the latter (some personal Re’s) is fact, would you mind to post the result(s) or a summary of personally received answers, if the former is true, it is to regret that obviously nobody has an answer or it is no more interesting.

As of my "memories" [legacy of ancillary knowledge] searching the web it might be there are solutions but prior to be a „ know it all“ I would like to ask you, dear Michal, whether your problem with enhancing the contrast in your cryosections (if possible: which tissue / specimens - biological, materials?, polymers? How your cryosections are collected from the knife edge [i.e. dry, wet by allowing to thaw on the grid] ? ) persists or / and what – if ever – answers to your original questions were.
For using OTO-method it is not too late but perhaps there are thinkable methods you should try in the first place....

Thanking in advance for your understanding,
best wishes and Season's Greetings

sincerely yours,



MUSS Wolfgang PhD (Dr. phil.)
[OR i. R. / en retraite / private / retired]
FRMS, MSA-member, Emeritus status
Ignaz-Rieder-Kai 19/6
A-5020 SALZBURG
Österreich-AUSTRIA
Mobil-Tel.: 0043(0)676 5 369 456
E-mail: wij.muss-at-aon.at
E-Mail altern.: womuss-at-gmail.com

====================================================================================
On Mi 14.06.2017 21:23 Michal Jarnik / NIH { michal.jarnik-at-nih.gov} wrote:
----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Dear listers,

Does anyone have experience with post-fixing Tokuyasu cryosections with osmium tetroxide? The purpose would be to gain better and more “standard” membrane contrast.
I tried to fix the grids on a drop of 2% OsO4, but there was very little (if any) change. I am sure people tried before, but do not remember reading about it. Maybe OTO enhancement or...?

Thanks for ideas,

Michal Jarnik
NIH
====================================================================================










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From: zaluzec-at-microscopy.com
Date: Mon, 19 Jun 2017 06:50:46 -0500
Subject: [Microscopy] viaWWW:

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Title-Subject: [Filtered] E.F. Fullam sputter coater head

Message: Does anyone know how I can procure a sputter coater head for an
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From: microscopy.listserver-at-gmail.com
Date: Mon, 19 Jun 2017 07:08:47 -0500
Subject: [Microscopy] viaWWW:E.F. Fullam sputter coater head

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Title-Subject: [Filtered] E.F. Fullam sputter coater head

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From: microscopy.listserver-at-gmail.com
Date: Mon, 19 Jun 2017 07:18:44 -0500
Subject: [Microscopy] viaWWW:E.F. Fullam sputter coater head

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Title-Subject: [Filtered] E.F. Fullam sputter coater head

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From: maryard-at-uga.edu
Date: Wed, 21 Jun 2017 09:38:54 -0500
Subject: [Microscopy] TEM - gold labeling protein complex

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The early registration deadline for M&M 2017 is just a few days away and as you are making plans to attend, please don't forget about MSA's student bursary program. Its purpose is to encourage students to attend the meetings by helping to defray some of the cost and giving them an opportunity to meet and interact with the established microscopy community.

The students will be paid $10 an hour to work for ~15-20 hours during the meeting or pre-meeting events (paid by check at end of meetings). The jobs involve providing support in the different symposia, staffing the volunteer office; newsletter distribution, or helping with vendor tutorial sign-up. There is an added bonus of a meeting t-shirt and $10 cash for each morning and/or afternoon session worked to assist with meals.

Volunteers are also needed to help with the above mentioned meeting tasks since not all will be filled by bursaries. Although not paid the hourly rate as the students, volunteers are given the same $10 cash to assist with meals and meeting t-shirt. They also have the opportunity to interact more with the microscopy community while assisting with the meeting.

If anyone would like to participate in the bursary program, would be willing to volunteer, or has any questions please contact Amanda Lawrence (alawrence-at-i2at.msstate.edu).

Don't forget the early registration deadline is June 26. Meetings are Aug. 6-10 in St. Louis, MO.

Amanda Lawrence
Institute for Imaging and Analytical Technologies
Mississippi State University
mailto:alawrence-at-i2at.msstate.edu




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From kimgarr459-at-gmail.com Tue Jun 20 12:42:48 2017
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I have a client who will be submitting outer surface proteins (OSPs) to resolve by TEM. He plans to use gold conjugated to the primary antibody which will express an antigen on these OSPs. Does anyone have a protocol or know of any older articles related to this? Some concerns and questions I have are: attachment of the OSPs onto a formvar (or parlodian) grid; using a routine IEM procedure or a revised protocol; using a routine negative stain (UA or PTA) or would low angle shadowing work more efficiently?
Thank you for any responses to this project!
Mary Ard, EM Lab Coordinator
University of Georgia, Georgia Electron Microscopy Facility
151 Barrow Hall
115 DW Brooks Drive, UGA Campus
Athens, GA 30602
maryard-at-uga.edu



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From: vlad_speransky-at-tedpella.com
Date: Wed, 21 Jun 2017 14:14:59 -0500
Subject: [Microscopy] TEM - gold labeling protein complex

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Mary Ad,

For uniform spreading of protein particles on the grid, it is best to have
glow-discharged carbon film on top of your formvar or parlodion. If that is
not an option, bacitracin solution often helps, or even BSA. Then you can
use any basic immunoEM protocol -- which you can obtain from any major
supplier or perhaps from community members here. Use minimum blocking first,
to establish some binding, then you can include extra blocking steps or
higher concentrations, if there is an objectionable background. This usually
works quite well for a protein laying on a grid, with no obstacles, like
when it is somewhere deep in the cell.

What species is the primary antibody from? If possible, I always preferred
to use Protein A -gold as a secondary probe. Much cleaner and more precise.
It is best with rabbit polyclonals and will not work with most mouse
monoclonals.

Finally, the contrasting step - go with PTA (or ammonium molybdate) over UA:
not as dark and spreads easier.

Let me know if you need a sample protocol.

Best wishes,
Vlad

Vlad Speransky, PhD
Life Sciences Product Specialist
Ted Pella, Inc.
http://www.tedpella.com/
530-243-2200 ext. 266
Cell 530-768-3953
vlad_speransky-at-tedpella.com





-----Original Message-----
X-from: maryard-at-uga.edu [mailto:maryard-at-uga.edu]
Sent: Wednesday, June 21, 2017 7:59 AM
To: vlad_speransky-at-tedpella.com

I have a client who will be submitting outer surface proteins (OSPs) to
resolve by TEM. He plans to use gold conjugated to the primary antibody
which will express an antigen on these OSPs. Does anyone have a protocol or
know of any older articles related to this? Some concerns and questions I
have are: attachment of the OSPs onto a formvar (or parlodian) grid; using
a routine IEM procedure or a revised protocol; using a routine negative
stain (UA or PTA) or would low angle shadowing work more efficiently?
Thank you for any responses to this project!
Mary Ard, EM Lab Coordinator
University of Georgia, Georgia Electron Microscopy Facility
151 Barrow Hall
115 DW Brooks Drive, UGA Campus
Athens, GA 30602
maryard-at-uga.edu



==============================Original Headers==============================
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From: PhillipsT-at-missouri.edu
Date: Wed, 28 Jun 2017 09:07:56 -0500
Subject: [Microscopy] cryostat suggestions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Good morning listers,

In the process of cleaning the lab, I came across a what appears to be a
complete MT-2 instruction manual. Any takers? Let me know within a week
or into the dumpster she goes.

Jim

--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
63B York St.
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/dmf

Two silk worms had a race.
They ended up in a tie.


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From thivisid08-at-gmail.com Fri Jun 23 19:34:15 2017
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Dear All,

From the lectures and discussions at the recent 3DEM GRC (Les
Diablerets, 2017), I noticed there are still serious misunderstandings –
even among distinguished professors in Physics and in Biology - on what
“resolution” actually means. So allow me to go over the basic principles:

1) The instrumental resolution of an imaging system is given by the
physical properties of the microscope, telescope, photographic camera or
whatever your favorite 1D-, 2D-, 3D-, 4D-imaging device is. The
classical case would be that of a light microscope where the numerical
aperture (NA) of the objective lens
(https://en.wikipedia.org/wiki/Numerical_aperture) determines the
“instrumental resolution” of the microscope
(https://en.wikipedia.org/wiki/Angular_resolution).
In the case of a diffraction-limited telescope it is the diameter of the
main lens that determines the instrumental resolution. In the old days
of Electron Microscopy one would often see the first zero of the CTF
being used to define the instrumental resolution of the microscope.

2) The resolution achieved for the results based on the images collected
is a very different issue! Suppose, for example, you forget to switch on
the illumination of your light microscope! What good will then the
high-resolution (high NA) properties of your expensive instrument do
you? If, on the other hand, you do switch on the illumination but only
use a very low dose of ~ 10,000 photons to generate an image, that image
will be very noisy. How much better will the image of your object be if
the image is created accumulating a total of 10,000,000,000 photons? The
underlying question is: how do I define a results-oriented quality
metric that reflects the image information I have collected in an
experiment rather than what a specific instrument can potentially collect?

The basic idea is to take TWO images of the same object rather than just
ONE. Both images will contain the same signal (the object) but a
different realization of the random noise so we can then compare the two
images to each other in Fourier space using the FRC (Fourier Ring
Correlation). This suggestion first emerged in single-particle EM in the
early 1980s (https://en.wikipedia.org/wiki/Fourier_shell_correlation).

Strangely enough it took decades for the rest of the imaging scientists
to realize what they were missing. Only very recently “everybody”
suddenly started using the results-oriented FRC and FSC metrics in many
other imaging fields, including X-ray microscopy, X-ray crystallography,
light-microscopy, X-ray tomography, scanning microscopy, astronomical
imaging, etc.

Instead of claiming “super resolution” by showing some nice images from
a given microscope, one can now just prove it through an FRC/FSC curve.
I never understood why it took everybody so long to adapt to this
straightforward gold-standard metric.

Take home lesson: the “INSTRUMENTAL RESOLUTION” is the intrinsic
resolution that the instrument is capable of, whether you actually use
it or whether you leave it in the cupboard. The statistically
significant “RESULTS RESOLUTION”, on the other hand, reflects the
quality of the final results achieved within a given data-collection
experiment. These are TWO very different concepts!

My TWO cents,

Marin

=====================================

Prof Dr Ir Marin van Heel
Research Professor at:
Laboratório Nacional de Nanotecnologia - LNNano
CNPEM/LNNano, Campinas, Brazil
Emeritus Professor, Imperial College London
Emeritus Professor, Leiden University
Skype: marin.van.heel






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From aaronlittle895-at-gmail.com Sun Jun 25 08:12:46 2017
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Message-ID: {D1F5A874.ECCF41FB-at-gmail.com}

We are thinking about buying a cryostat for our core facility. Some clients really want the cryojane add on kit. Do any of you use this in a core setting? What do you charge for consumables?

How important do you think a motorized drive is? One or two clients want this expensive upgrade - does it get used by the average person? Is it worth the money?

Leica has a vacuum assist in their 1950 model that aids in sectioning. I haven't seen the demo yet but intend to. Any fans of this technology?

Thanks. Tom



Thomas E. Phillips, Ph.D
Professor of Biological Sciences
Curator's Distinguished Teaching Professor
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400
573-882-4712 (office)
573-882-0123 (fax)
phillipst-at-missouri.edu

http://www.biology.missouri.edu/faculty/phillips.html
http://microscopy.missouri.edu



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From: arvind-at-nbrc.ac.in
Date: Thu, 29 Jun 2017 06:49:00 -0500
Subject: [Microscopy] Re: cryostat suggestions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,
we have ordered one with Cryojane tape transfer system on Leica CM3050S 2
-3 years ago but in vain as the company was not able to do the successful
DEMO even after multiple efforts finally it had to be used without the
cryojane system also the accessories will cost you lot, I think only LEICA
only claims to have this technology

thanks,

Pundir (TO)
National Brain Research Centre
Manesar, Gurgaon
HARYANA
INDIA



----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

We are thinking about buying a cryostat for our core facility. Some
clients really want the cryojane add on kit. Do any of you use this in a
core setting? What do you charge for consumables?

How important do you think a motorized drive is? One or two clients want
this expensive upgrade - does it get used by the average person? Is it
worth the money?

Leica has a vacuum assist in their 1950 model that aids in sectioning. I
haven't seen the demo yet but intend to. Any fans of this technology?

Thanks. Tom



Thomas E. Phillips, Ph.D
Professor of Biological Sciences
Curator's Distinguished Teaching Professor
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400
573-882-4712 (office)
573-882-0123 (fax)
phillipst-at-missouri.edu

http://www.biology.missouri.edu/faculty/phillips.html
http://microscopy.missouri.edu



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From: gary-at-gaugler.com
Date: Mon, 3 Jul 2017 02:54:40 -0500
Subject: [Microscopy] Dimethoxypropane source

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

RMC-Boeckeler makes a tape-transfer system for their microtomes with cryo.

Phil
-------------
Philip Oshel
Imaging Facility Director
Biology Department
1304 Biosciences
1455 Calumet Ct.
Central Michigan University
Mt. Pleasant, MI 48859
989 774-3576 office
989 774-7567 lab









-----Original Message-----
X-from: "arvind-at-nbrc.ac.in" {arvind-at-nbrc.ac.in}
Reply-To: "arvind-at-nbrc.ac.in" {arvind-at-nbrc.ac.in}

Hi,
we have ordered one with Cryojane tape transfer system on Leica CM3050S 2
-3 years ago but in vain as the company was not able to do the successful
DEMO even after multiple efforts finally it had to be used without the
cryojane system also the accessories will cost you lot, I think only LEICA
only claims to have this technology

thanks,

Pundir (TO)
National Brain Research Centre
Manesar, Gurgaon
HARYANA
INDIA



----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

We are thinking about buying a cryostat for our core facility. Some
clients really want the cryojane add on kit. Do any of you use this in a
core setting? What do you charge for consumables?

How important do you think a motorized drive is? One or two clients want
this expensive upgrade - does it get used by the average person? Is it
worth the money?

Leica has a vacuum assist in their 1950 model that aids in sectioning. I
haven't seen the demo yet but intend to. Any fans of this technology?

Thanks. Tom



Thomas E. Phillips, Ph.D
Professor of Biological Sciences
Curator's Distinguished Teaching Professor
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400
573-882-4712 (office)
573-882-0123 (fax)
phillipst-at-missouri.edu

http://www.biology.missouri.edu/faculty/phillips.html
http://microscopy.missouri.edu



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From otiscatr45-at-gmail.com Sat Jul 1 23:44:58 2017
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Has anyone found a source for this dehydration agent? Can
you share the source ID with the List? I need this agent to prep
some bio specimens the right way.

Your help and insight is much appreciated.

gary g.


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Date: Mon, 3 Jul 2017 07:30:58 -0500
Subject: [Microscopy] viaWWW:Hitachi S800 Manual and Service manual Request

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Email: lbochtler-at-protonmail.ch Name: Lukas B.

Title-Subject: [Filtered] Hitachi S800 Manual and Service manual Request

Message: Hello,

I just scored a Hitachi S800 electron microscope, sadly most cables between the main console (with
the electron column) and the control desk have been cut. I can replace most if not all cables, so
that isn't much of a problem.

However, i did not get any documentation with it, since i don't know if the original owner will ever
manage to find any documentation for it, i decided to ask you guys.

Ideally i would need both the user and service manual for the microscope, possibly with the computer
software (since im not sure if i can get the computer for it as well, since the microscope and the
other parts are now in 2 different locations)

Ideally id also need the power requirements for the microscope, what voltage dose it need, how much
power dose it draw, and how many power phases dose it need.


Best regards,

Lukas B.

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From: microscopy.listserver-at-gmail.com
Date: Wed, 5 Jul 2017 18:18:30 -0500
Subject: [Microscopy] viaWWW:Job offering - Project Manager Nanotechnology (f/m)

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Dear Gary,

just to inform (knowing there might have been meanwhile a lot of personal
replies to you):
hope to have understood your request correctly.
almost all bigger chemical sellers*) have DMP-Dimethoxypropane in stock (I
guess youll use it for rapid dehydration of biological specimens avoiding
the common dehydration by ascending ethanol or acetone steps ( acidified DMP
will dehydrate small biological specimens by cleaving tissular water
molecules into methanol and acetone).

==============================================
*) Google search: | dimethoxypropane msds |
==============================================
Companies (as examples, disclaimer see below):
Science Lab, SIGMA-ALDRICH; MILLIPORE(MERCK); ChemSpider, ACROS-FISHER
SCIENTIFIC; ALFA AESAR

-----------------------------------------------
Science Lab:
www.sciencelab.com/msds.php?msdsId=9923794
Sciencelab.com, Inc.
14025 Smith Rd.
Houston, Texas 77396
US Sales:
1-800-901-7247
International Sales:
1-281-441-4400
Order Online:
ScienceLab.com

-----------------------------------------------
SIGMA-ALDRICH
(for Austria:
http://www.sigmaaldrich.com/catalog/product/sial/d136808?lang=de®ion=AT&g
clid=CMKbk-yD7dQCFdAV0wodKHwCnA
Globally: www.sigmaaldrich.com
Austria Home
D136808 Sigma-Aldrich 2,2-Dimethoxypropane reagent grade, 98%
Synonym: Acetone dimethyl acetal
- CAS Number 77-76-9
- Linear Formula (CH3)2C(OCH3)2
- Molecular Weight 104.15
- Beilstein Registry Number 635678
- EC Number 201-056-0
- MDL number MFCD00008479
- PubChem Substance ID 24893272

Related Categories Analytical/Chromatography, Applications, Chemical
Synthesis, Derivatization of Fatty Acids to FAMEs, Fats (fatty acids, FAMEs,
glycerides), Edible Oils, Sterols,
More...
Grade: reagent grade
vapor density: 3.59 (vs air)
vapor pressure: 60 mmHg ( 15.8 C)
InChI Key: HEWZVZIVELJPQZ-UHFFFAOYSA-N
Assay: 98%
expl. lim. 31%, 58F
6%, 27F

Packaging
18 L in steel drum
25, 500 mL in glass bottle
2.5 L in glass bottle
Application
Reagent for the preparation of 1,2-diols as acetonides.[3]
General description
2,2-Dimethoxypropane (DMP) is an organic building block commonly employed as
a precursor to generate 2-methoxypropene (MPP). The degradation study of DMP
in ionic liquids showed the formation of MPP and 2-ethoxypropene (EPP) in an
identical ratio due to the tunneling effect.[4] Conformational analysis of
DMP based on ab initio calculations and matrix isolation infrared
spectroscopy has been reported.[1] DMP reacts with water to produce methanol
and acetone. This reaction has been employed in a method for the
quantification of water in natural products by gas-liquid chromatography.[5]
Acidified DMP has been employed for the dehydration of biological
samples.[2]

-----------------------------------------------
http://www.merckmillipore.com/AT/de/product/2%2C2-Dimethoxypropane,MDA_CHEM-
802936?ReferrerURL=https%3A%2F%2Fwww.google.com%2F
802936 | 2,2-Dimethoxypropan
- Download
- Zoom
2,2-Dimethoxypropane for synthesis. CAS 77-76-9, molar mass 104.15g/mol.
2,2-Dimethoxypropan MSDS (material safety data sheet) or SDS, CoA and CoQ,
dossiers, brochures and other available documents.

-----------------------------------------------
2,2-Dimethoxypropane | C5H12O2 | ChemSpider
www.chemspider.com/Chemical-Structure.21106033.html

-----------------------------------------------
ACROS, Fisher Scientific:
https://www.fishersci.com/shop/products/2-2-dimethoxypropane-98-acros-organi
cs-4/p-4258969


-----------------------------------------------
ALFA AESAR:
https://www.alfa.com/en/catalog/A13810/
A13810 2,2-Dimethoxypropane, 98%
Use: 2,2-Dimethoxypropane acts as a dehydrating agent. It also serves as an
intermediate in the synthesis of vitamin E, vitamin A and various
carotenoids such as astaxanthin. It is used as a reagent for the preparation
of 1,2-diols, acetonides, isopropylidene derivatives of sugars, nucleosides,
methyl esters of amino acids and enol ethers.
Notice:
Incompatible with oxidizing agents and acids.

============================================================================
==========
Disclaimer:
Just to be honest: no affiliation with / no financial interest in any of
the mentioned companies.
I have used acidified DMP in/for the rapid dehydration of small biological
(human) tissue specimens in diagnostic TEM for nearly 30 years with really
good success (some points may be critical and perhaps need to be mentioned
at least) and do have some (routine/standard recipes for that)

Literature (only a selection out of many):
CONWAY K 1999, https://www.ncbi.nlm.nih.gov/pubmed/10190257
DeRUITER A, VanBANNING P& WILLEMSE JJ (1981): Brief technical note
Rapid histological results in aquaculture research by using the time-saving
embedding procedure with 2,2-dimethoxypropane
( Aquaculture, Volume 25, Issues 23, August 1981, Pages 293-297) see:
http://www.sciencedirect.com/science/article/pii/0044848681901939
KAESER W. 1989. Freeze substitution of plant tissues with a new medium
containing dimethoxypropane. J. Microsc. 154: 273-278
LIN C.H., Falk R.H. and Stocking C.R. 1977. Rapid chemical Ddehydration of
plant material for light and electron microscopy with 2,2-dimethoxypropane
and 2,2-diethoxypropane. Am. J. Bot.64: 602-605
MASER D.M. and TRIMBLE III, J.J. 1977. Rapid chemical dehydration of
biologic samples for scanning electron microscopy using
2,2-dimethoxypropane. J. Histochem. Cytochem. 25:247-251
MASON M, MACKIE RM (1985): Comparative study of three methods of plastic
embedding in diagnostic dermatopathology JClinPathol1985;38:1397-1399,


(https://www.ncbi.nlm.nih.gov/pmc/articles/PMC499499/pdf/jclinpath00195-0077
.pdf)
MLLER W. and MLLER G. 1994. Chemical dehydration for rapid paraffin
embedding. Biotech. & Histochem. 69: 289-290
MULLER L.L. and JACKS T.J. 1975. Rapid chemical dehydration of samples for
electronmicroscopic examinations. J. Histochem. Cytochem. 23: 107-110
A Pernstich, H W Krenn, G Pass(2003): Preparation of serial sections of
arthropods using 2,2-dimethoxypropane dehydration and epoxy resin embedding
under vacuum. Biotechnic & Histochemistry, Jan 2003, Vol. 78, No. 1, Pages
5-9.
( http://www.tandfonline.com/doi/abs/10.1080/10520290312120002 )

POSTEK M. T. and TUCKER S. C. 1976. A new short chemical dehydration method
for light microscopy preparations of plant material. Can. J. Bot. 54,
872-875



Best wishes and regards,

MUSS Wolfgang Dr.phil. (PhD)
[OR i. R. / en retraite / retired]
Ignaz-Rieder-Kai 19/6
A-5020 SALZBURG
sterreich-AUSTRIA
Mobil-Tel.: 0043(0)676 5 369 456
FN-Tel. m.
Anrufbeantw.: 004(0)662 62 91 46
E-mail: wij.muss-at-aon.at
E-Mail altern.: womuss-at-gmail.com

FRMS, Retired Member of MSA

Scientific Profile at ResearchGate:
http://www.researchgate.net/profile/Wolfgang_MUSS
Former Head of Electron Microscopy Lab at Institute of Pathology
SALK-LKH / Salzburger Landeskliniken | General Hospital and
PMU (private) PARACELSUS MEDICAL UNIVERSITY of SALZBURG

Former Secretary and (until June2017) Board Member of the

SCUR
{The Society for Cutaneous Ultrastructure Research}
The Skin Imaging Society { www.scur.org }

Von: gary-at-gaugler.com [mailto:gary-at-gaugler.com]
Gesendet: Montag, 3. Juli 2017 10:14
An: wij.muss-at-aon.at
Betreff: [Microscopy] Dimethoxypropane source

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Your help and insight is much appreciated.

gary g.


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Email: thomas.glaeser-at-leica-microsystems.com Name: Thomas Glaeser

Organization: Leica Microsystems GmbH

Title-Subject: [Filtered] Job offering - Project Manager Nanotechnology (f/m)

Message: For our Product Management team in Vienna we are looking for a strong personality as

Product Manager Nanotechnologie (f/m)

Key Responsibilities:

• Manage the product life cycle for assigned products within the Biological portfolio
• Project management of internal efforts required for product commercialization, including
interfacing with Quality Management Systems, R&D, and related roles
• Organize and drive activities cross functionally related to global product launch, including
developing positioning, sales training, pricing and collateral development to ensure product launch
execution
• Interact with key internal and external customers globally to understand problems, obtain feedback
on new and existing products, and identify solutions
• Specify market requirements from all major geographic regions supported by on-going market
research with customers and non-customers
• Monitors financial performance of the (revenue, profitability) market, and establishes KPI’s for
the product line’s financial performance and develops countermeasures when performance is not met
Key Requirements:

• Bachelor's degree in Science, Engineering, Marketing, Business Administration, or equivalent
experience is required. PhD or MBA is preferred
• Minimum 3-5 years of experience in research / sample preparation of biological samples Strong
knowledge of EM and EM sample preparation with cryo methods
• Experience in product management, marketing preferred
• Proficient in English and German (spoken & written), other languages (is a plus)

Are you interested to work independently in a team orientated environment, do you want to go new
ways and solve innovative issues? Please apply directly at
https://danaher.taleo.net/careersection/jobdetail.ftl?job=NAN000025&lang=en
or send me an email.


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From: frank.macaluso-at-einstein.yu.edu
Date: Thu, 6 Jul 2017 12:34:38 -0500
Subject: [Microscopy] JEOL 100CX apertures

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have an assortment of new apertures for a JEOL 100CX II. They will also fit a 100CX.
The assortment includes fixed condenser apertures and condenser and objective aperture strips. They are yours for the asking. Just pay shipping.

Frank Macaluso
Senior Associate in Anatomy and Structural Biology
Administrative Director and Director of Electron Microscopy
Analytical Imaging Facility
Albert Einstein College of Medicine
1300 Morris Park Avenue
Bronx, NY 10461

(718) 430-3547
frank.macaluso-at-einstein.yu.edu
http://www.einstein.yu.edu/aif

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3, 157 -- Subject: JEOL 100CX apertures
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From: microscopy.listserver-at-gmail.com
Date: Thu, 6 Jul 2017 16:02:59 -0500
Subject: [Microscopy] viaWWW:Aspex Controller box and Stage

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Email: rcsencsits-at-belcan.com Name: Roseann Csencsits

Organization: Schafer Corporation-A Belcan Company

Title-Subject: [Filtered] Aspex Controller box and Stage

Message: Does anyone have an Aspex 3025 joystick controller box and/or
stage? I am looking for the Aspex Explorer 80 x 100 stage and controller
box. the stage is motorized x,y and manual z. I believe many have been
decommissioned and I am interested in parts. Thanks, Roseann

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9, 50 -- Subject: viaWWW:Aspex Controller box and Stage
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From: microscopy.listserver-at-gmail.com
Date: Mon, 10 Jul 2017 08:55:21 -0500
Subject: [Microscopy] viaWWW:Alternatives to Essen BioScience Incucyte

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Email: mtg2003-at-med.cornell.edu Name: Michael Ganger

Organization: Weill Cornell Medicine

Title-Subject: [Filtered] EMBED 812 Bubbles

Message: Hi quick question for everyone. I usually cure my EMBED 812
resin in a vacuum oven at 15mmHg at 60C. The oven had failed
facilitating a new purchase. After balancing the temperature, I put
some test blocks in without tissue to just test the polymerization and
cutting post cure.

To my dismay, I have been having bubbles form in the capsules (BEEM 00),
and they occur along the edges from the bottom to the top of capsule.
It does not matter where I put the trays, or the number of capsules to
polymerize. I have varied the vacuum from 10mmHg (bubbles are finer and
worse) to 20 mmHg (bubbles form at the bevel to the tip and are slightly
larger in size.)I have yet to vary the temp.

I've been using vacuum ovens for over 16 years and have never had any
problems before and I'm a bit stumped. Any help would be most appreciated.

Thanks in advance,

Mike
Weill Cornell Medicine
NY, NY

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From skarbnik-at-ugdl.pl Sun Jul 9 03:10:56 2017
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Email: D.Strachan-at-Beatson.gla.ac.uk Name: David Strachan

Organization: Beatson Institute for Cancer Research

Title-Subject: [Filtered] Alternatives to Essen BioScience Incucyte

Message: Dear List,

I was wondering if anyone has any thoughts on alternatives to the Essen
Bioscience Incucyte system.
Essentially we would like a system that can accommodate multi-well
plates, image for up to one week, so probably placed inside an incubator
or have its own specialised environmental control. Be capable of phase
contrast or other I.C. technique and have a minimum of two colour
fluorescence.

The new incucyte S3 now has an over-inflated price hike, at least in the
U.K., so is no longer looking like something we will be considering.

Any thoughts greatly appreciated.

regards

David Strachan
Senior Scientific Officer
Beatson Institute for Cancer Research
UK



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From: Stephen.Beck-at-ncc.edu
Date: Mon, 10 Jul 2017 15:16:04 -0500
Subject: [Microscopy] Philips EM-300

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html









Dear All
During SEM examination of human endometrium I found a filamentous structure
resembling bacteria. The way it contacts the microvilli of the epithelial
cells looks like this object was there before fixation, and I can see
constrictions (sulci) along the filament. So I think it is a colony but I
would like to ask your opinion, as some of you are very strong in observing
bacteria with SEM. I could not take higher resolution images because the
sample has difficulties and the electron beam was drifting.
Please have a look:

http://www.eikonika.net/yorgos.html

Thank you for your time
yorgos

Dr Yorgos Nikas
Athens Innovative Microscopy
Skra 36 Voula 16673 GREECE

Tel/fax +30 210 8957677
mobile +30 6945 107477
www.eikonika.netwww.aim.cat
*************************************





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8, 20 -- Subject: filamentous bacteria?
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From ebeldong6-at-gmail.com Mon Jul 10 15:01:25 2017
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Message-ID: {6875F14E.882AB665-at-gmail.com}

Dear Colleagues,

If anyone has expertise on the EM-300 TEM, please contact me offline. Specifically, I believe I have a water flow issue (the vacuum ON light remains illuminated) and a vacuum problem related to the camera airlock (possibly one of the microswitches that interface with the airlock mechanism/rotary knob).

Thanks,

Steve

Stephen J. Beck
Professor
Coordinator, Bio-Imaging Center
Electron Microscopy
Department of Biology
Nassau Community College
Garden City, NY 11530

Sent from my iPhone - thanks Steve (1955-2011)!

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From: COURYHOUSE-at-aol.com
Date: Mon, 10 Jul 2017 15:57:59 -0500
Subject: [Microscopy] Looking for Manuals, Parts and peoples memories of the RCA EMT (EMT-3)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

SMECC Museum project in Arizona is looking for Manuals, Parts, photos
of installations and people using them and peoples memories of the RCA EMT
(EMT-3)

Greetings, in our many year effort to document the RCA EMT Entry level
Electron Microscope we are looking basically for anything related to it
including memories and folklore.

Please drop us a line off list. if you have any of the above to share.

Many thanks in advance....
Ed Sharpe Archivist for SMECC www.smecc.org

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From: kerry.thompson-at-nuigalway.ie
Date: Thu, 13 Jul 2017 09:10:03 -0500
Subject: [Microscopy] New MSc in Microscopy & Imaging - National University of Ireland

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Dear all,

We would like to draw your attention to a new Master of Science course starting in National University of Ireland Galway. This is an exciting new course and will benefit from the cutting edge facilities available from the Centre for Microscopy and Imaging at NUIG. At a time when we can view and control the very actions of cells using light, follow the development of cells in vivo and even visualise individual molecular interactions in a biological context this course will equip students with invaluable skills for a multitude of careers and research applications.

NUIG is ranked among the top 1% of universities globally in the QS World University Ranking 2017/18, and this ranking has been consistently rising for the past 5 years. Galway as a city is a bustling hub of festivals and culture it was voted "Most Charming" by the NY Times, "Friendliest" city by The US Travel & Leisure Magazine, "Europe's Micro City of the Year" by The Financial Times and the European Capital of Culture for 2020.

Applications are being taken on a rolling basis. Please see link below and circulate to any parties you feel may be interested in applying..

MSc in Microscopy & Imaging
http://www.nuigalway.ie/microscopy-imaging/

Many thanks

Dr Kerry Thompson

Kerry Thompson PhD,
Lecturer,
Anatomy,
School of Medicine,
NUI Galway.
P: +353(0)91495704

Secretary, Microscopy Society of Ireland - www.microscopy.ie
Fellow, Outreach & Education Committee, Royal Microscopical Society - www.rms.org.uk



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10, 58 -- Subject: New MSc in Microscopy & Imaging - National University of Ireland
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From: microscopy.listserver-at-gmail.com
Date: Fri, 14 Jul 2017 08:44:00 -0500
Subject: [Microscopy] viaWWW: EMS Microscopy Academy is pleased to announce the Biological

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Email: stacie-at-ems-secure.com Name: Stacie Kirsch

Organization: Electron Microscopy Sciences

Title-Subject: [Filtered] Biological TEM Workshop, Personal Short Courses and more

Message: The EMS Microscopy Academy is pleased to announce the Biological TEM Workshop will be held
November 7-9.

This course offers a complete picture of proper chemical processing of biological samples for TEM
observation, teaching both theory and hands-on preparation using buffers, fixatives, and other
solutions. Participants are encouraged to bring their own samples to get expert advice on the work
they need to do back in their own lab.

Additional classes are forming for:
Pharmaceutical Microscopy
X-Ray Microanalysis
Cryosectioning/ImmunoGold
and more!

Don't see the topic you're interested in? Fill out our online form to request a topic, or schedule
a 1-2 day Personal Short Course! Equipment Demos are also available for you to try before you buy.


For more information, visit our website at www.emsdiasum.com and follow the link to EMS Microscopy
Academy to learn more!

Thanks,
Stacie Kirsch

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From: microscopy.listserver-at-gmail.com
Date: Fri, 14 Jul 2017 08:45:11 -0500
Subject: [Microscopy] viaWWW:Elemental analysis by SEM-EDS

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Email: ravi.thakkar369-at-gmail.com Name: Ravi

Title-Subject: [Filtered] Elemental analysis by SEM-EDS.

Message: Hi listeners,
I have a few questions for EDS analysis using Electron microscope. I did one soil sample for one
user, the composition of soil was unknown. So, they did SEM-EDS to know the composition of sample.
1. What's the difference between weight% & atomic% value in EDS report?


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From: microscopy.listserver-at-gmail.com
Date: Fri, 14 Jul 2017 15:02:27 +0000
Subject: [Microscopy] viaWWW:Elemental analysis by SEM-EDS

Contents Retrieved from Microscopy Listserver Archives
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The answer to your stated question is simple. The answer to your unstated question is more complicated.

Mass fraction or weight fraction is just that. How many grams of that element would you find in 100 grams of samples.
Atomic fraction converts that over to moles so you might determine stoichiometry and the formula, if appropriate.
For example, pyrite should be 46.6 wt% Fe and 53.4 wt% S. If convert that to moles, you would find it is 33.3 at% Fe and 66.7 at% S which tells you there are two atoms of S for every atom of Fe. The formula is FeS2.

Now the unstated questions should be "Was the analysis done properly?" and "How accurate was the analysis?"
The analysis was probably done on a rough powder preparation. How well did that represent the original sample? What effect did that have on the accuracy of the analysis? The best analyses are from flat, homogenous samples. I don't suppose the soil was only one phase. How did you handle oxygen in the sample; how well does your detector measure oxygen?

In short, the analysis will give a ballpark figure at best. I would not necessarily expect the same answer from two different preparations of the same sample. Caution your user about pushing the results too far.

Warren Straszheim

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Title-Subject: [Filtered] Elemental analysis by SEM-EDS.

Message: Hi listeners,
I have a few questions for EDS analysis using Electron microscope. I did one soil sample for one user, the composition of soil was unknown. So, they did SEM-EDS to know the composition of sample.
1. What's the difference between weight% & atomic% value in EDS report?


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Title-Subject: [Filtered] Elemental analysis by SEM-EDS.

Message: Hi listeners,
I have a few questions for EDS analysis using Electron microscope. I did one soil sample for one user, the composition of soil was unknown. So, they did SEM-EDS to know the composition of sample.
1. What's the difference between weight% & atomic% value in EDS report?


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Subject: [Microscopy] viaWWW:Elemental analysis by SEM-EDS

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Hi Ravi,

Atomic percentage (or ratio) and weight percentage are two ways of describing the chemical composition of a compound.
For example, for water:

the atomic ratio is 2 H for each O (67% H and 33% O)
the weight percentage is (total weight of water molecule is 18) 2/18= 11% H and 16/18= 89% O

As you can see, it is very important to know which one you are using because the percentage values are very different.

You can find more information here:

https://en.wikipedia.org/wiki/Atomic_ratio

https://en.wikipedia.org/wiki/Mass_fraction_(chemistry)

Please note that for an EDS analysis you also have the option to normalize the results (so that the sum of the element you are looking for is always 100%).

Regards,
Stefano

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Email: ravi.thakkar369-at-gmail.com Name: Ravi

Title-Subject: [Filtered] Elemental analysis by SEM-EDS.

Message: Hi listeners,
I have a few questions for EDS analysis using Electron microscope. I did one soil sample for one user, the composition of soil was unknown. So, they did SEM-EDS to know the composition of sample.
1. What's the difference between weight% & atomic% value in EDS report?


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From: microscopy.listserver-at-gmail.com
Date: Sun, 16 Jul 2017 11:51:39 -0500
Subject: [Microscopy] viaWWW:Elemental analysis by SEM-EDS

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X-from: Stefano Rubino {stefano-at-soquelec.com}

Hi Ravi,

Atomic percentage (or ratio) and weight percentage are two ways of describing the chemical
composition of a compound.
For example, for water:

the atomic ratio is 2 H for each O (67% H and 33% O)
the weight percentage is (total weight of water molecule is 18) 2/18= 11% H and 16/18= 89% O

As you can see, it is very important to know which one you are using because the percentage values
are very different.

You can find more information here:

https://en.wikipedia.org/wiki/Atomic_ratio

https://en.wikipedia.org/wiki/Mass_fraction_(chemistry)

Please note that for an EDS analysis you also have the option to normalize the results (so that the
sum of the element you are looking for is always 100%).

Regards,
Stefano

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Title-Subject: [Filtered] Elemental analysis by SEM-EDS.

Message: Hi listeners,
I have a few questions for EDS analysis using Electron microscope. I did one soil sample for one
user, the composition of soil was unknown. So, they did SEM-EDS to know the composition of sample.
1. What's the difference between weight% & atomic% value in EDS report?

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From: microscopy.listserver-at-gmail.com
Date: Sun, 16 Jul 2017 11:52:15 -0500
Subject: [Microscopy] viaWWW:Elemental analysis by SEM-EDS

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X-from: Murowchick, James {MurowchickJ-at-umkc.edu}


Ravi,
For individual mineral grain analyses, the atomic % is useful for identifying some minerals based
on their stoichiometry since it gives the number of atoms of a particular element per 100 atoms
total. Calcite, CaCO3 has 5 atoms, so it has 20 at. % Ca, 20 at % C, and 60 at. % O. Weight % is
the mass of each element measured per the total mass. Calcite has molecular mass of (40.08 +
12.011+3*15.999) = 100.088g/mol, so it has 40.08/100.088 % Ca = 40.04 wt % Ca, and so on. For a
soil or other mixture, the EDS spectrum should be collected over a large enough area that the
results are representative of the bulk composition. Atomic % might not be very useful unless the
numbers of each element are needed, but such results are often given in terms of wt %. Conversion
from elemental wt % to atomic % or wt % as oxides (common for rock analyses) is relatively
straightforward and described on most mineralogy textbooks.

I hope this helps.

Jim
Dr. James B. Murowchick
Professor, Geochemistry & Mineralogy
Principal Graduate Advisor & IPhD Coordinator, Geosciences
Department of Geosciences
University of Missouri-Kansas City
420 Flarsheim Hall 5110 Rockhill Road Kansas City, MO 64110
Office: 816 235-2979
Department Office: 816 235-1334
Fax: 816 235-5535
murowchickj-at-umkc.edu



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Title-Subject: [Filtered] Elemental analysis by SEM-EDS.

Message: Hi listeners,
I have a few questions for EDS analysis using Electron microscope. I did one soil sample for one
user, the composition of soil was unknown. So, they did SEM-EDS to know the composition of sample.
1. What's the difference between weight% & atomic% value in EDS report?

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From: microscopy.listserver-at-gmail.com
Date: Tue, 18 Jul 2017 07:07:25 -0500
Subject: [Microscopy] viaWWW:Assistant Position available-Rockefeller University

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X-from: Erico Freitas {freitas.erico-at-gmail.com}




Hi Ravi,


The basic difference between wt% and at% in the EDS report the way the software calculates the
composition.
In soil, there are several Si-, Al-, and Fe- (hydr)oxide phases in its composition. So, be careful
with the EDS quantification report, as you cannot quantify light elements (Z {11) by using EDS.
There's always delocalized electrons contributing to the X-ray emission for light elements. For
oxides, it's better to quantify oxygen in such samples indirectly, by running a quantification model
that takes into account the charge balance.
Regarding the w% of at%, it's up to you.

Cheers,

On Fri, Jul 14, 2017 at 11:06 AM, {microscopy.listserver-at-gmail.com
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Email: ravi.thakkar369-at-gmail.com {mailto:ravi.thakkar369-at-gmail.com} Name: Ravi

Title-Subject: [Filtered] Elemental analysis by SEM-EDS.

Message: Hi listeners,
I have a few questions for EDS analysis using Electron microscope. I did one soil sample for one
user, the composition of soil was unknown. So, they did SEM-EDS to know the composition of sample.
1. What's the difference between weight% & atomic% value in EDS report?


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--
Erico Freitas
Físico - Centro de Microscopia da UFMG
Currículo Lattes: *http://lattes.cnpq.br/8786127123101199*
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Email: kuryu-at-rockefeller.edu Name: Kunihiro Uryu

Organization: The Rockefeller University, Electron Microscopy Resource Center

Title-Subject: [Filtered] Assistant Position available

Message: Dear List,

The Rockefeller University, New York, NY, seeks outstanding candidates for Electron Microscopy
Resource Center (EMRC). The EMRC provides state of the art electron microscopy support for analysis
of a wide variety of biological samples, including virus, bacteria, insects, animal tissue as well
as cultured cell and isolated cellular components for structural analyses or immuno-electron
microscopy. The EMRC is equipped with two TEMs and one SEM as well as a high pressure freezing and a
free substitution unit. Please visit our website for more detail. (http://www.rockefeller.edu/emrc/)

Job Title: Electron Microscopists: Research Support Assistant
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Department Description:
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facilities, providing expert electron microscopy services and training to researchers for The
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Job Responsibilities:
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work, sample preparation for transmission and scanning electron microscopy and maintenance of the
center. Will be responsible for conventional sample preparation, including processing samples,
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Please visit the following site to submit your application: Job: IRC20260

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From: microscopy.listserver-at-gmail.com
Date: Tue, 18 Jul 2017 07:12:31 -0500
Subject: [Microscopy] viaWWW:JEOL JEM 1010 parts wanted

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Email: semy66-at-yahoo.fr Name: Sami ZEKRI

Organization: TEM Lab. Faculty of Medicine- Tunis- Tunisia

Title-Subject: [Filtered] JEOL JEM 1010

Message: Hi every one,

I'm looking for a refurbished / old electronic " Panel ITF PB " card for JEOL JEM 1010 microscope.


Thanks

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From: microscopy.listserver-at-gmail.com
Date: Wed, 19 Jul 2017 07:53:58 -0500
Subject: [Microscopy] viaWWW:JEOL 840A help needed

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Email: gary-at-cermetmaterials.com Name: Gary Castelow

Organization: Cermet Materials

Title-Subject: [Filtered] JEOL 840A

Message: Hello all! I got some good help from here before so we decided to try again. We recently
had a power blip/surge/loss? last Friday. We noticed late Monday afternoon our SEM was shut down.
It would not turn back on with the key, so we checked the power box in the other room and the reset
was tripped. We flipped it back on the tried the key again and it fired right up...for about
25-30secs. In the manual it says after about 30 secs the DP power supply will turn on. I reset the
instrument and tried to fire it up again and watched the light panel, sure enough as soon as the DP
light turns on the SEM shuts down. Bad power supply? Maybe shorted or fried? If so where is this
power supply and how could I check it in house? Any other ideas of what it could be?

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From: diller-at-stefan-diller.com
Date: Wed, 19 Jul 2017 11:16:06 -0500
Subject: [Microscopy] Re: viaWWW:JEOL 840A help needed

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Hi Gary,


Did you check all the fuses at the power supply (the big yelloish block) ?

If you suspect the heater plate of the diffusion pump, take away the panels, unplug the heater and measure the resistance. I think
it should be ca. 30 - 50 ohm.

When the SEM shuts down at start of DP heating, you might have a faulty heater plate. You will need to contact Jeol to get a
fitting one (ca. 400 ...).

You can try unplug the heater and start the SEM. If it starts then you know; but: it will switch off later since the thermo-switch
at the DP will not close after a certain time (normally 20 minutes)

What can also be a reason for the Jeol to switch off is:

- not reaching the pre-vacuum trip point (check your pre-vac pump suction pressure), faulty vacuum lines (I once had a leak just
in between the vibration dampening weight...)

- no sufficient air pressure at the SEM (4 bar...)


Best,

Stefan

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Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
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Websites:
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Anfahrt: http://Mail.map24.com/Stefan.Diller
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Am 19.07.17 um 15:16 schrieb microscopy.listserver-at-gmail.com:
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} Title-Subject: [Filtered] JEOL 840A
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} Message: Hello all! I got some good help from here before so we decided to try again. We recently
} had a power blip/surge/loss? last Friday. We noticed late Monday afternoon our SEM was shut down.
} It would not turn back on with the key, so we checked the power box in the other room and the reset
} was tripped. We flipped it back on the tried the key again and it fired right up...for about
} 25-30secs. In the manual it says after about 30 secs the DP power supply will turn on. I reset the
} instrument and tried to fire it up again and watched the light panel, sure enough as soon as the DP
} light turns on the SEM shuts down. Bad power supply? Maybe shorted or fried? If so where is this
} power supply and how could I check it in house? Any other ideas of what it could be?
}
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18, 30 -- From diller-at-stefan-diller.com Wed Jul 19 11:16:06 2017
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From: mdelann1-at-jhmi.edu
Date: Thu, 20 Jul 2017 08:36:12 -0500
Subject: [Microscopy] molecular sieve

Contents Retrieved from Microscopy Listserver Archives
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Mississippi State University is seeking a Research Associate - Microscopy

Mississippi State University's (MSU), Institute for Imaging & Analytical Technologies (I2AT) is a university level research institute and core facility, meeting MSU's missions in research, education, and service. In order to properly serve the needs of the life-sciences and engineering research community, the I2AT requires a Research Associate to assist with diverse applications in biological-sample preparation, imaging, characterization and research. This individual will function at the professional level and will collaborate with researchers involved in experimental design, implementation, and analysis of a wide variety of life-science samples (e.g. micro-organisms in culture, in tissues and bio-films plus animal and plant tissues).

ESSENTIAL DUTIES & RESPONSIBILITIES
The individual to be hired will be expected to have significant experience in biological materials sample preparation, imaging, and characterization. In particular, we are seeking an individual with experience in light, electron (both scanning and transmission), and confocal laser scanning microscopies. Preference will be given to individuals with experience with these techniques as they relate to biological-materials research.

MINIMUM QUALIFICATIONS
The successful candidate must have, at a minimum, a MS in a life-sciences related field with emphasis and experience in biological research to include sample preparation for both scanning and transmission electron microscopies, as well as imaging and characterization.

Strong communication and writing skills and the ability to function effectively in a team environment is required. As an I2AT Research Associate, this person will have the opportunity to publish collaboratively and independently in his/her area of expertise. The Research Associate will also be given the opportunity to be involved in grant proposal development and implementation. The I2AT Research Associate will work with the Director to implement performance/career development planning and review.

PREFERRED QUALIFICATIONS
* PhD in Life-Sciences field, minimum 2 years of experience in biological electron microscopy
* MS in Life-Sciences field, 3-5 year of experience in biological electron microscopy
* Cryo sample preparation
* Cryo electron microscopy experience

If interested click or copy and paste this link: http://explore.msujobs.msstate.edu/cw/en-us/job/495702/research-associate-iiiiii-or-senior



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9, 32 -- From zrowland-at-i2at.msstate.edu Wed Jul 19 11:29:37 2017
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From rosaronald70-at-gmail.com Wed Jul 19 19:12:35 2017
Return-Path: {rosaronald70-at-gmail.com}
Received: from gmail.com (mail.dongnamdc.com [222.97.39.100] (may be forged))
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Message-ID: {076E67CA.21355118-at-gmail.com}

Hello,
I was wondering if there are any labs out there that use molecular sieved
200 proof ethanol for dehydrating TEM samples? Do you prewash them in
ethanol and then bake out before use? I used to do this but switched to
the pint sized bottles (opening up a new one for each new exp). The problem
of course is generating too many bottles of unused ethanol. All comments
are welcome. If you do use it please forward a catalog number and vendor.

Thank you,
Michael Delannoy


==============================Original Headers==============================
3, 20 -- From prvs=36781e466=mdelann1-at-jhmi.edu Thu Jul 20 08:36:11 2017
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3, 20 -- To: {Microscopy-at-microscopy.com} , {microscopy-at-msa.microscopy.com}
3, 20 -- Subject: molecular sieve
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From: microscopy.listserver-at-gmail.com
Date: Thu, 20 Jul 2017 08:39:44 -0500
Subject: [Microscopy] viaWWW:Hummer X, Plasma cleaner repair

Contents Retrieved from Microscopy Listserver Archives
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X-from: kattymansouri-at-gmail.com

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Email: kattymansouri-at-gmail.com Name: Katayoun Mansouri

Organization: Duke University

Title-Subject: [Filtered] Hummer X, Plasma cleaner repair

Message: Hello,
We have an old Hummer X sputter coater/plasma cleaner that needs to be repaired. Does anyone know or
know of somebody that could do that for us? We are at Duke University, and prefer someone local.
Thanks

Katayoun

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From: microscopy.listserver-at-gmail.com
Date: Thu, 20 Jul 2017 08:46:03 -0500
Subject: [Microscopy] viaWWW:JEOL 840A help needed

Contents Retrieved from Microscopy Listserver Archives
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To: microscopy.listserver-at-gmail.com, gary-at-cermetmaterials.com

Hello Gary,
It sounds like DP heater problem. There is two diffusion pumps under the column of your SEM, a small
one and a bigger one ; The heaters are at the bottom and are connected to 200V by a ceramic plug.
Shutdown the SEM, unplug the ceramic plug and check if the two pins are still isolated from the
ground. If not, there is a short circuit and you may buy another heater. Such problem appears often
when the SEM shutdown and when the water still flow in the pump hoses. Humidity of the room and
temperature gap between water and air act together to condensate water on the body of the pumps.
This water fall on the heater...
Hope this is help.

Nicolas STEPHANT

Université de Nantes
Institut Jean Rouxel
Service de microscopie électronique à balayage et microanalyse
2 rue de la Houssinière
BP 92208
44322 Nantes cédex 3

Tél : 02 40 37 64 26
Mél : nicolas.stephant-at-univ-nantes.fr
Web : http://cnrs-imn.fr

"Le monde n'existe que pour autant que nous sommes capables d'en produire une image"
C.G Jung

Le 19/07/2017 à 15:07, microscopy.listserver-at-gmail.com a écrit :
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} Title-Subject: [Filtered] JEOL 840A
}
} Message: Hello all! I got some good help from here before so we decided to try again. We recently
} had a power blip/surge/loss? last Friday. We noticed late Monday afternoon our SEM was shut down.
} It would not turn back on with the key, so we checked the power box in the other room and the reset
} was tripped. We flipped it back on the tried the key again and it fired right up...for about
} 25-30secs. In the manual it says after about 30 secs the DP power supply will turn on. I reset the
} instrument and tried to fire it up again and watched the light panel, sure enough as soon as the DP
} light turns on the SEM shuts down. Bad power supply? Maybe shorted or fried? If so where is this
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From: microscopy.listserver-at-gmail.com
Date: Thu, 20 Jul 2017 08:46:48 -0500
Subject: [Microscopy] viaWWW:JEOL 840A help needed

Contents Retrieved from Microscopy Listserver Archives
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X-from: Hank Beebe {HBeebe-at-rjleegroup.com}
To: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com}

Gary,
Check the indicators on the RP.
Check the thermo stw on the DP



Hank Beebe
Manager, Instrument Services
RJ Lee Group

724.325.1776 Office
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HBeebe-at-rjleegroup.com

Website | Lab Services | Shop | Pay My Bill

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Email: gary-at-cermetmaterials.com Name: Gary Castelow

Organization: Cermet Materials

Title-Subject: [Filtered] JEOL 840A

Message: Hello all! I got some good help from here before so we decided to try again. We recently
had a power blip/surge/loss? last Friday. We noticed late Monday afternoon our SEM was shut down.
It would not turn back on with the key, so we checked the power box in the other room and the reset
was tripped. We flipped it back on the tried the key again and it fired right up...for about
25-30secs. In the manual it says after about 30 secs the DP power supply will turn on. I reset the
instrument and tried to fire it up again and watched the light panel, sure enough as soon as the DP
light turns on the SEM shuts down. Bad power supply? Maybe shorted or fried? If so where is this
power supply and how could I check it in house? Any other ideas of what it could be?

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From: oshel1pe-at-cmich.edu
Date: Thursday, 20July, 2017 at 09:59
Subject: [Microscopy] molecular sieve

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Michael,

I have used molecular sieve in 100% EtOH for years, it works well.
I use mSorb sieves, and they are packaged to be used as received - that is, no pre-baking before use, nor do they need washing in ethanol before use. Which is good, because unless the ethanol is absolutely anhydrous, some of the water capacity of the sieve would be used up in the ethanol wash, and then you would have to pre-bake.
Just watch the dust - be careful getting EtOH out of the bottle with sieve in it, or put the sieve in dialysis tubing.

I ordered these from Delta Absorbents, Cat #MS3AEDG05 in 5 gal pails (*much* cheaper than buying from lab supply or EM supply companies) and MSBI4A4801 for the blue indicating sieve (1 lb packets). The indicating sieve is *much* more expensive, so buy a separate packet and mix it 1:5 or 1:10 with the non-indicating sieve.

Phil
-------------
Philip Oshel
Imaging Facility Director
Biology Department
1304 Biosciences
1455 Calumet Ct.
Central Michigan University
Mt. Pleasant, MI 48859
989 774-3576 office
989 774-7567 lab



microscopy-at-microscopy.com {microscropy-at-microscopy.com}




-----Original Message-----
X-from: "mdelann1-at-jhmi.edu" {mdelann1-at-jhmi.edu}
Reply-To: "mdelann1-at-jhmi.edu" {mdelann1-at-jhmi.edu}

Hello,
I was wondering if there are any labs out there that use molecular sieved
200 proof ethanol for dehydrating TEM samples? Do you prewash them in
ethanol and then bake out before use? I used to do this but switched to
the pint sized bottles (opening up a new one for each new exp). The problem
of course is generating too many bottles of unused ethanol. All comments
are welcome. If you do use it please forward a catalog number and vendor.

Thank you,
Michael Delannoy




-------------
Philip Oshel
Imaging Facility Director
Biology Department
1304 Biosciences
1455 Calumet Ct.
Central Michigan University
Mt. Pleasant, MI 48859
989 774-3576 office
989 774-7567 lab






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26, 55 -- From: "Oshel, Philip Eugene" {oshel1pe-at-cmich.edu}
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26, 55 -- CC: "mdelann1-at-jhmi.edu" {mdelann1-at-jhmi.edu}
26, 55 -- Subject: Re: [Microscopy] molecular sieve
26, 55 -- Thread-Topic: [Microscopy] molecular sieve
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From: mdelann1-at-jhmi.edu
Date: Thu, 20 Jul 2017 12:20:16 -0500
Subject: [Microscopy] molecular sieve

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,
Many thanks to all your quick responses to my molecular sieve questions. I
think we will give Phil Oshel's protocol a shot:
Order a 5 1b sieve and 1 lb indicator sieve, mix 1:5. From Delta
adsorbents. Ready to use no washing or baking required (I like that).

Again many thanks,
Michael Delannoy


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3, 21 -- Subject: molecular sieve
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From: acoritz-at-emsdiasum.com
Date: Thursday, 20July, 2017 at 09:59
Subject: [Microscopy] molecular sieve

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Residual molecular sieves will kill your diamond knife if it makes it into your block. Known problem with using this to dry ETOH.


Al Coritz
Applications & Service Manager
Electron Microscopy Sciences
1560 Industry Road
Hatfield, PA 19440
acoritz-at-emsdiasum.com
800-523-5874





-----Original Message-----
X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu]
Sent: Thursday, July 20, 2017 11:35 AM
To: Al Coritz {acoritz-at-emsdiasum.com}

Michael,

I have used molecular sieve in 100% EtOH for years, it works well.
I use mSorb sieves, and they are packaged to be used as received - that is, no pre-baking before use, nor do they need washing in ethanol before use. Which is good, because unless the ethanol is absolutely anhydrous, some of the water capacity of the sieve would be used up in the ethanol wash, and then you would have to pre-bake.
Just watch the dust - be careful getting EtOH out of the bottle with sieve in it, or put the sieve in dialysis tubing.

I ordered these from Delta Absorbents, Cat #MS3AEDG05 in 5 gal pails (*much* cheaper than buying from lab supply or EM supply companies) and MSBI4A4801 for the blue indicating sieve (1 lb packets). The indicating sieve is *much* more expensive, so buy a separate packet and mix it 1:5 or 1:10 with the non-indicating sieve.

Phil
-------------
Philip Oshel
Imaging Facility Director
Biology Department
1304 Biosciences
1455 Calumet Ct.
Central Michigan University
Mt. Pleasant, MI 48859
989 774-3576 office
989 774-7567 lab



microscopy-at-microscopy.com {microscropy-at-microscopy.com}




-----Original Message-----
X-from: "mdelann1-at-jhmi.edu" {mdelann1-at-jhmi.edu}
Reply-To: "mdelann1-at-jhmi.edu" {mdelann1-at-jhmi.edu}

Hello,
I was wondering if there are any labs out there that use molecular sieved
200 proof ethanol for dehydrating TEM samples? Do you prewash them in
ethanol and then bake out before use? I used to do this but switched to
the pint sized bottles (opening up a new one for each new exp). The problem
of course is generating too many bottles of unused ethanol. All comments
are welcome. If you do use it please forward a catalog number and vendor.

Thank you,
Michael Delannoy




-------------
Philip Oshel
Imaging Facility Director
Biology Department
1304 Biosciences
1455 Calumet Ct.
Central Michigan University
Mt. Pleasant, MI 48859
989 774-3576 office
989 774-7567 lab






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From: oshel1pe-at-cmich.edu
Date: Thursday, 20July, 2017 at 09:59
Subject: [Microscopy] molecular sieve

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Very true!
Not a problem I’ve had, but then it’s just a matter of being careful to not stir up the sieve when in ethanol - or of putting the sieve in dialysis tubing.

Phil
-------------
Philip Oshel
Imaging Facility Director
Biology Department
1304 Biosciences
1455 Calumet Ct.
Central Michigan University
Mt. Pleasant, MI 48859
989 774-3576 office
989 774-7567 lab









-----Original Message-----
X-from: Al Coritz {acoritz-at-emsdiasum.com}

Michael,

I have used molecular sieve in 100% EtOH for years, it works well.
I use mSorb sieves, and they are packaged to be used as received - that is, no pre-baking before use, nor do they need washing in ethanol before use. Which is good, because unless the ethanol is absolutely anhydrous, some of the water capacity of the sieve would be used up in the ethanol wash, and then you would have to pre-bake.
Just watch the dust - be careful getting EtOH out of the bottle with sieve in it, or put the sieve in dialysis tubing.

I ordered these from Delta Absorbents, Cat #MS3AEDG05 in 5 gal pails (*much* cheaper than buying from lab supply or EM supply companies) and MSBI4A4801 for the blue indicating sieve (1 lb packets). The indicating sieve is *much* more expensive, so buy a separate packet and mix it 1:5 or 1:10 with the non-indicating sieve.

Phil
-------------
Philip Oshel
Imaging Facility Director
Biology Department
1304 Biosciences
1455 Calumet Ct.
Central Michigan University
Mt. Pleasant, MI 48859
989 774-3576 office
989 774-7567 lab



microscopy-at-microscopy.com {microscropy-at-microscopy.com}




-----Original Message-----
X-from: "mdelann1-at-jhmi.edu" {mdelann1-at-jhmi.edu}
Reply-To: "mdelann1-at-jhmi.edu" {mdelann1-at-jhmi.edu}

Hello,
I was wondering if there are any labs out there that use molecular sieved
200 proof ethanol for dehydrating TEM samples? Do you prewash them in
ethanol and then bake out before use? I used to do this but switched to
the pint sized bottles (opening up a new one for each new exp). The problem
of course is generating too many bottles of unused ethanol. All comments
are welcome. If you do use it please forward a catalog number and vendor.

Thank you,
Michael Delannoy




-------------
Philip Oshel
Imaging Facility Director
Biology Department
1304 Biosciences
1455 Calumet Ct.
Central Michigan University
Mt. Pleasant, MI 48859
989 774-3576 office
989 774-7567 lab






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From: microscopy.listserver-at-gmail.com
Date: Thu, 20 Jul 2017 15:57:57 -0500
Subject: [Microscopy] viaWWW:JEOL 840A help needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Bill Mushock {wim5-at-lehigh.edu}


Most likely your DPs are full of water due to condensation from the cooling water. If you dry them
out, they may work but most likely need replacing.

On Thu, Jul 20, 2017 at 9:49 AM, {microscopy.listserver-at-gmail.com
{mailto:microscopy.listserver-at-gmail.com} } wrote:




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To: microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} ,
gary-at-cermetmaterials.com {mailto:gary-at-cermetmaterials.com}

Hello Gary,
It sounds like DP heater problem. There is two diffusion pumps under the column of your SEM, a
small
one and a bigger one ; The heaters are at the bottom and are connected to 200V by a ceramic plug.
Shutdown the SEM, unplug the ceramic plug and check if the two pins are still isolated from the
ground. If not, there is a short circuit and you may buy another heater. Such problem appears
often
when the SEM shutdown and when the water still flow in the pump hoses. Humidity of the room and
temperature gap between water and air act together to condensate water on the body of the pumps.
This water fall on the heater...
Hope this is help.

Nicolas STEPHANT

Université de Nantes
Institut Jean Rouxel
Service de microscopie électronique à balayage et microanalyse
2 rue de la Houssinière
BP 92208
44322 Nantes cédex 3

Tél : 02 40 37 64 26
Mél : nicolas.stephant-at-univ-nantes.fr {mailto:nicolas.stephant-at-univ-nantes.fr}
Web : http://cnrs-imn.fr

"Le monde n'existe que pour autant que nous sommes capables d'en produire une image"
C.G Jung

Le 19/07/2017 à 15:07, microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com}
a écrit :
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} Email: gary-at-cermetmaterials.com {mailto:gary-at-cermetmaterials.com} Name: Gary Castelow
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} Organization: Cermet Materials
}
} Title-Subject: [Filtered] JEOL 840A
}
} Message: Hello all! I got some good help from here before so we decided to try again. We
recently
} had a power blip/surge/loss? last Friday. We noticed late Monday afternoon our SEM was shut
down.
} It would not turn back on with the key, so we checked the power box in the other room and the
reset
} was tripped. We flipped it back on the tried the key again and it fired right up...for about
} 25-30secs. In the manual it says after about 30 secs the DP power supply will turn on. I
reset the
} instrument and tried to fire it up again and watched the light panel, sure enough as soon as
the DP
} light turns on the SEM shuts down. Bad power supply? Maybe shorted or fried? If so where is
this
} power supply and how could I check it in house? Any other ideas of what it could be?
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--
William J Mushock
SEM Manager
Lehigh University
Whitaker Laboratory
5 East Packer Ave.
Bethlehem, PA 18015
610-758-4283

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From: microscopy.listserver-at-gmail.com
Date: Fri, 21 Jul 2017 17:56:55 -0500
Subject: [Microscopy] viaWWW:Postdoctoral Fellow/Research Associate Position Available

Contents Retrieved from Microscopy Listserver Archives
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X-from: bassimn-at-mcmaster.ca


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Email: bassimn-at-mcmaster.ca Name: Nabil Bassim

Organization: McMaster University

Title-Subject: [Filtered] Postdoctoral Fellow/Research Associate Position Available

Message: The Bassim group at McMaster University is seeking a Research Associate or Postdoctoral
Fellow in Microscopy

McMaster University has a strong electron microscopy effort, centered around the Canadian Centre for
Electron Microscopy, which is a state-of-the-art national facility located on the campus of McMaster
University. This position would leverage the individual's skills with electron microscopy methods in
materials science to use advanced aberration-corrected instruments to research a variety of
materials systems, including traditional metallurgical alloys, nanomaterials, and electronic thin films.

ESSENTIAL DUTIES & RESPONSIBILITIES
The individual to be hired will be expected to have significant experience in materials
characterization using transmission electron microscopy and such techniques as diffration-methods,
TEM, STEM, EDS and EELS. In particular, we seek an individual with experience in using
aberration-corrected (S)TEM methods, and with a versatile background.

MINIMUM QUALIFICATIONS
The successful candidate must have a Ph.D in a materials-sciences related field with emphasis and
experience in transmission electron microscopy, with experience in imaging, diffraction and
spectroscopy.
Strong communication and writing skills and the ability to function effectively in a team
environment is required. This person will have the opportunity to publish collaboratively and
independently in his/her area of expertise with the balance worked out depending on available
funding and time. The Research Associate will also be given the opportunity to be involved in grant
proposal development and implementation.
PREFERRED QUALIFICATIONS
* Aberration-corrected TEM experience
* Sample preparation capabilities
* Familiarity with metallurgical microstructures
* willingness to mentor younger graduate students.


If you are interested, please email Prof. Nabil Bassim (bassimn-at-mcmaster.ca). If you are onsite at
M&M in August, please mention this in your email of interest as there is an opportunity for a 1-1
interview in St. Louis.

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From: microscopy.listserver-at-gmail.com
Date: Jul 10, 2017 10:31 AM
Subject: [Microscopy] viaWWW:Alternatives to Essen BioScience Incucyte

Contents Retrieved from Microscopy Listserver Archives
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X-from: David Strachan {d.strachan-at-beatson.gla.ac.uk}
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Email:D.Strachan-at-Beatson.gla.ac.uk {mailto:D.Strachan-at-Beatson.gla.ac.uk} Name: David Strachan

Organization: Beatson Institute for Cancer Research

Title-Subject: [Filtered] Alternatives to Essen BioScience Incucyte

Message: Dear List,

I was wondering if anyone has any thoughts on alternatives to the Essen
Bioscience Incucyte system.
Essentially we would like a system that can accommodate multi-well
plates, image for up to one week, so probably placed inside an incubator
or have its own specialised environmental control. Be capable of phase
contrast or other I.C. technique and have a minimum of two colour
fluorescence.

The new incucyte S3 now has an over-inflated price hike, at least in the
U.K., so is no longer looking like something we will be considering.

Any thoughts greatly appreciated.

regards

David Strachan
Senior Scientific Officer
Beatson Institute for Cancer Research
UK

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From: gilpin-at-purdue.edu
Date: Mon, 24 Jul 2017 09:47:44 -0500
Subject: [Microscopy] rotary shadowing with cressington 308R

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Hi everyone,
If anyone is using a Cressington 308R for rotary shadowing I would love to hear from you offline to talk about methods.
​​​​​
Chris

Christopher J. Gilpin Ph.D.
Director, Life Science Microscopy Facility
Campus-wide Coordinator for Electron Microscopy
Purdue University
Whistler Hall of Agriculture Research, Room S052
170 S. University St
West Lafayette, IN 47907
765-494-7750
gilpin-at-purdue.edu
lsmf-at-purdue.edu reaches everyone in the facility.
http://ag.purdue.edu/arp/Microscopy/Pages/default.aspx




==============================Original Headers==============================
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From: microscopy.listserver-at-gmail.com
Date: Tue, 25 Jul 2017 07:57:15 -0500
Subject: [Microscopy] viaWWW:Automated and Rapid Specimen Processing Course Sept 11-12,

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Electron Microscopy Technician

A technical position is available in the Core Microscopy Facility of The Scripps Research Institute in La Jolla, California. The facility houses electron microscopes (TEM & SEM), as well as confocal (laser scanning & spinning disc), multi-photon, TIRF / STORM and widefield systems. The successful candidate will assist with the operations of the electron microscopy resources (TEM and SEM) of the TSRI Core Microscopy Facility. Duties will include the undertaking electron microscopy projects, assisting users of the facility, providing basic instruction in the use of the electron microscopes, sample preparation (TEM & SEM), minor equipment maintenance (e.g. filament changes) and some administrative work (ordering of supplies and monthly billing). Applicants should have excellent communication and organizational skills, an understanding of laboratory procedures, and the ability to manage a large and varied workload. Minimum qualifications include a bachelor’s degree in Science (with a concentration in Biology), at least 5 years of hands-on experience with electron microscopy, and sample preparation (including sectioning). Computer skills are essential.

To apply, go to the listing on TSRI HR careers website at: https://careers.scripps.edu/postings/11052

or the search the general employments listings (https://careers.scripps.edu/postings/search), using requisition number 04063

-----------------------

Scott Henderson, Ph.D.
Professor, Dept. Molecular Medicine
Director, TSRI Microscopy Core
MB-22
The Scripps Research Institute
10550 North Torrey Pines Rd.
La Jolla, CA 92037


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From gregw-at-gsk.com Tue Jul 25 02:56:13 2017
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Email: stacie-at-ems-secure.com Name: Stacie Kirsch

Organization: Electron Microscopy Sciences

Title-Subject: [Filtered] Discover the Endless Possibilities... at EMS Microscopy Academy

Message: EMS Microscopy Academy is pleased to announce the Automated and Rapid Specimen Processing
Course, which introduces participants to the use of equipment for processing tissue samples for TEM.
It is scheduled for Monday and Tuesday, September 11 and 12, in Hatfield, PA.

The preparation of samples for EM requires many steps, with extended wait times in between,
requiring an entire day of a technician’s time. This process consists of several fixative, wash,
dehydration, and resin infiltration steps, which are tedious and prone to temporal variability
between runs. With the use of an automated tissue processor or microwave, a technician’s time is
reduced and better continuity between processing runs is obtained.

To learn more about the class, please copy and paste this webpage into your browser.
http://www.emsdiasum.com/microscopy/academy/courses/rapid_processing.aspx

Hope to have you join us!
Stacie Kirsch

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From: oshel1pe-at-cmich.edu
Date: Tue, 25 Jul 2017 09:06:36 -0500
Subject: [Microscopy] FW: Ask a Microscopist Aperture vs Spot Size vs Spatial Resolution

Contents Retrieved from Microscopy Listserver Archives
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***********************************************************************************
Forwarded from "Ask a Microscopist"
Please remember that the person asking the question is likely
not a member the listserver, and
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as well as to the list.
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Name:Ryan Wilmington

School:College of William & Mary

Grade/Education Level:Undergraduate

US Email:rlwilmington-at-email.wm.edu


What is the relationship between the size of the aperture used before the objective, and the ultimate spot size and spatial resolution of an IR microscope?





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From: microscopy.listserver-at-gmail.com
Date: Fri, 28 Jul 2017 11:23:28 -0500
Subject: [Microscopy] viaWWW:Research Support Assistant Position available Rockefeller

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,

If you have a spare SEM filament assembly you are willing to part with please reply to me. I am building an electron flood gun for a high vacuum (thermal) metal evaporator.

Tungsten filament type preferred.

Thank you!

John Elzey
UNSW Physics

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From ronaldfaust205-at-gmail.com Wed Jul 26 08:38:31 2017
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Email: kuryu-at-rockefeller.edu Name: Kunihiro Uryu

Organization: The Rockefeller University, Electron Microscopy Resource Center

Title-Subject: [Filtered] Assistant Position available

Message: Dear List,

The Rockefeller University, New York, NY, seeks outstanding candidates for Electron Microscopy
Resource Center (EMRC). The EMRC provides state of the art electron microscopy support for analysis
of a wide variety of biological samples, including virus, bacteria, insects, animal tissue as well
as cultured cell and isolated cellular components for structural analyses or immuno-electron
microscopy. The EMRC is equipped with two TEMs and one SEM as well as a high pressure freezing and a
free substitution unit. Please visit our website for more detail. (http://www.rockefeller.edu/emrc/)


Job Title: Electron Microscopists: Research Support Assistant
Employment status: Full time

Department Description:
The Electron Microscopy Resource Center (EMRC) is one of the University’s scientific core
facilities, providing expert electron microscopy services and training to researchers for The
Rockefeller University and neighboring institutions. The EMRC provides state- of-the-art electron
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structural analyses, immuno-electron microscopy, CLEM, SEM serial block face imaging, montage, and
tomography. The EMRC is equipped with three TEMs and two SEM as well as a high pressure freezing and
other ambient and cryo EM sample preparation devices.

Job Responsibilities:
Will support the Electron Microscopy Resource Center's (EMRC) daily operations, including bench
work, sample preparation for transmission and scanning electron microscopy and maintenance of the
center. Will be responsible for conventional sample preparation, including processing samples,
cutting ultrathin sections, data collection, ordering and receiving supplies, and managing chemical
waste compliance and administrative support for office duties.


Job Requirements:
Bachelor's degree in science required. Educational emphasis in biology, cell biology, bioengineering
or a related field preferred. Must have strong communication skills, ability to work as part of a
team, and flexibility to interact with a diverse group of researchers. Must be detail-oriented,
focused, highly motivated, and able to work in a team atmosphere for general lab duties, as well as
a mentored atmosphere for wide variety research projects. EM hands on experience preferred, but not
required. Competency in use of computers is plus, including functional command of Word,
Excel,PowerPoint and Photoshop.

Please visit the following site to submit your application: Job: IRC20260

https://recruit.rockefeller.edu/OA_HTML/OA.jsp?page=/oracle/apps/irc/candidateSelfService/webui/VisVacDispPG&OAHP=IRC_EXT_SITE_VISITOR_APPL&OASF=IRC_VIS_VAC_DISPLAY&akRegionApplicationId=821&transactionid=1559920286&retainAM=N&addBreadCrumb=RP&p_svid=20260&p_spid=725778&oapc=5&oas=nrAX5ZlxoR5W4jSauqbKYg

The Rockefeller University is an Equal Opportunity Employer with a policy that forbids
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termination) on the basis of race, color, religion, sex, age, national origin, citizenship status,
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20, 53 -- Subject: viaWWW:Research Support Assistant Position available Rockefeller
20, 53 -- University
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From: microscopy.listserver-at-gmail.com
Date: Fri, 28 Jul 2017 11:24:21 -0500
Subject: [Microscopy] viaWWW:Permanent Position EM Research / Lab management NRL

Contents Retrieved from Microscopy Listserver Archives
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Email: rhonda.stroud-at-nrl.navy.mil Name: Rhonda Stroud

Organization: NRL

Title-Subject: [Filtered] Permanent Position EM Research / Lab management

Message: Permanent Federal Employee Position Available--- The Nanoscale Materials Section at the US
Naval Research Laboratory in Washington, DC has an immediate opening for a full-time research
scientist / EM lab manager. The Nanoscale Materials Section maintains the DoD’s premier electron
microscopy facilities for basic research, including a Nion UltraSTEM 200-X for imaging and
spectroscopy at scales down to the single atom, a JEOL 2200FS for in situ analysis, and a fully
accessorized FEI Helios G3 FIB-SEM. The ideal candidate for this position has aberration-corrected
STEM experience, and enjoys pushing the instrumentation to its performance limits. The job duties
will be split between collaborative research on nanoscale materials development, and microscope
maintenance / trouble-shooting / technique development. A Ph.D. in physics, materials science,
chemistry, geology or related field, and US citizenship are required. Interested candidates should
email a CV and publication list to rhonda.stroud-at-nrl.navy.mil.

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From: microscopy.listserver-at-gmail.com
Date: Fri, 28 Jul 2017 11:25:22 -0500
Subject: [Microscopy] viaWWW: FIB Technician Opening

Contents Retrieved from Microscopy Listserver Archives
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Email: ming_j_zhang-at-amat.com Name: Ming Zhang

Organization: Applied Materials Company

Title-Subject: [Filtered] FIB Technician Opening

Message: Applied Materials Company has an immediate opening for an FIB technician in Portland, Oregon.

Applied Materials Company is the world's leading semiconductor equipment manufacturing company. It
is one of top 500 U.S. Companies and has repeatedly been honored as "World’s Most Ethical Company"
and "100 Best Places to Work in Information Technology".

The successful candidate will cover night shift and mainly focus on TEM sample preparations by
hands-on operations of ex-situ and in-situ lift-out systems. He or she must be able to fabricate
{100 nm thin lamella with high success rate for TEM observations.
A minimum of Bachelor degree or equivalent combination of education and experience in Materials
Science, Engineering, Chemistry, or Physics, etc. is required.

US citizen preferred.

Interested individuals should send their resumes to Drew_Goettler-at-amat.com

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From: microscopy.listserver-at-gmail.com
Date: Mon, 31 Jul 2017 18:30:35 -0500
Subject: [Microscopy] viaWWW: postdoctoral position in TEM is available at the University

Contents Retrieved from Microscopy Listserver Archives
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X-from: pmccurdy-at-colostate.edu

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Email: pmccurdy-at-colostate.edu Name: Patrick R McCurdy

Organization: Colorado State University

Title-Subject: [Filtered] WetSEM Capsules

Message: I am curious if anyone has had experience with the WetSEM Capsules being marketed by EMS?
If anyone with positive or negative experience could email me off-line that would be greatly
appreciated.

Thanks,
Pat McCurdy

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From buviregi380-at-gmail.com Sun Jul 30 06:00:14 2017
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Email: andy.stewart-at-ul.ie Name: Andy Stewart

Organization: University of Limerick

Title-Subject: [Filtered] Amended advert

Message: Apologies for reposting, the direct weblink in the previous posting does not work.
Title: Postdoctoral position in electron microscopy of pharmaceutical crystals.
A 3-year postdoctoral position in TEM is available at the University of Limerick, Ireland
As part of the H2020 award MagnaPharm (https://www.magnapharm.com) the aim of this research
project is to control polymorphism in pharmaceutical crystal growth via magnetic fields, and to
solve crystal structure and morphology of these compounds using electron imaging and diffraction
methods as well as to build a matrix of machine conditions for data evaluation on organic crystals.
Work will be carried out using a double corrected, monochromated Titan Themis, equipped with EDS
and EELS, K2-IS and Oneview detectors, as well as in-situ holders (DENS lightning double tilt , DENS
ocean, DENS atmosphere, Fischione cryo-transfer holder.) Please apply here:
ul.ie
Vacancies
Job ID: 023368

Closing date is the 11th of August 2017.
For further information please contact Ursel.Bangert-at-ul.ie or Andy.Stewart-at-ul.ie

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From: microscopy.listserver-at-gmail.com
Date: Tue, 1 Aug 2017 10:34:05 -0500
Subject: [Microscopy] viaWWW:Problem with sectioning Flat embedded sample

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Email: leandro.lemgrubersoares-at-glasgow.ac.uk Name: Leandro Lemgruber

Organization: University of Glasgow

Title-Subject: [Filtered] Problem with sectioning Flat embedded sample

Message: Dear All,

I'm having a specific problem while trying to section a Flat embedded
sample (in glass bottom petri dishes) in Epoxy resin.
When the section hits the water, the resin expands, and keep expanding
until it reaches almost 3-4 times the size of the block face. And the
sections are quite sticky as well, making impossible to collect them (they
stay glued to the eyelash).
I have already processed samples before without a problem, but for the past
months it has been the same problem. I don't think its the resin because
cell pellets embedded in the same resin can be sectioned normally.
Any advise would be great.

Thanks!

Leandro

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From: microscopy.listserver-at-gmail.com
Date: Tue, 1 Aug 2017 19:11:25 -0500
Subject: [Microscopy] viaWWW:Problem with sectioning Flat embedded sample

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Michael Delannoy {mdelann1-at-jhmi.edu}
To: microscopy.listserver-at-gmail.com, delannoy-at-jhmi.edu

Leandro,
It sounds like an incomplete infiltration of your resin. Normally for cells
on coverslips, we do an overnight in pure epon (after complete a dehydration
series), no ethanol:epon parts. The next day we do 3 -2 hr changes of fresh
resin (rock, 15 psi vacuum, rock), then cure the resin/coverslip to a
pre-filled beem capsules and bake 60C overnight. The following morning we
detach the coverslip from the polymerized block by submerging in liquid
nitrogen. The coverslips fall off within 5-10 min. Cells are at the beem
capsule surface.


Sincerely,
Michael Delannoy

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Email: leandro.lemgrubersoares-at-glasgow.ac.uk Name: Leandro Lemgruber

Organization: University of Glasgow

Title-Subject: [Filtered] Problem with sectioning Flat embedded sample

Message: Dear All,

I'm having a specific problem while trying to section a Flat embedded sample
(in glass bottom petri dishes) in Epoxy resin.
When the section hits the water, the resin expands, and keep expanding until
it reaches almost 3-4 times the size of the block face. And the sections are
quite sticky as well, making impossible to collect them (they stay glued to
the eyelash).
I have already processed samples before without a problem, but for the past
months it has been the same problem. I don't think its the resin because
cell pellets embedded in the same resin can be sectioned normally.
Any advise would be great.

Thanks!

Leandro

Login Host: 130.209.127.194
Listserver Email Form V - 20120416
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From: hsia627-at-hotmail.com
Date: Wed, 2 Aug 2017 15:49:36 -0500
Subject: [Microscopy] Basic ultramicrotomy course announcement - 09/14 to 09/15 2017

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues,

We are pleased to announce that the Electron Microscopy Core Imaging Facility (EMCIF) at the University of Maryland Baltimore will be offering a Basic Ultramicrotomy mini-course on September 14th and 15th, 2017. This course is designed to teach novice users of ultramicrotome operation at room temperature, trimming and sectioning of biological specimen embedded in epoxy or acrylic resin. It can also serve as a refresher course for current ultramicrotome operators as the course covers the instrumentation, handling and choosing diamond knives and troubleshooting.

The course will include lectures, demonstration and (lots of) hands on practice. Registration will be limited to a maximum of six participants. More information regarding to the course and registration can be found inour website

http://www.dental.umaryland.edu/core-imaging/workshops-and-courses/umbultramicrotomycourse/

Best regards,

Ru-ching Hsia
Associate Professor and Director
rhsia-at-umaryland.edu
Electron Microscopy Core Imaging Facility
University of Maryland, Baltimore
http://www.dental.umaryland.edu/Core-imaging



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From: jacob.kabel-at-ubc.ca
Date: Thu, 3 Aug 2017 10:53:12 -0500
Subject: [Microscopy] Re: viaWWW:EBSD Question

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Organization: University of Idaho

Title-Subject: [Filtered] EBSD

Message: Regarding a metallic sample. I read that the sample should have the Normal direction facing
the EBSD detector (at 70 degrees) for pole figures, but does the normal direction need to be facing
the detector for Euler, Schmid, Normal, Transverse, and Rolling maps?
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From normanatlas54-at-gmail.com Thu Aug 3 04:06:45 2017
Return-Path: {normanatlas54-at-gmail.com}
Received: from gmail.com (mail.dongnamdc.com [222.97.39.100] (may be forged))
by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v7396g70024317
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Message-ID: {9c7801d30bf2$78970270$b1b93524-at-normanatlas54}
Reply-To: "Perry Fox" {normanatlas54-at-gmail.com}

Hi Taylor,

The coordinate systems are a matter of convention rather than any
particular technical need. A lot of samples that have EBSD performed on
them aren't rolled. The important thing when making pole figures and
maps is that you can relate the data supplied (and any anisotropy
observed) back to the greater context of your sample in some way. If
you don't care about how the maps relate to that greater context for
whatever reason, then the directions won't really matter to you.

-Jacob

On 2017-08-02 07:36 PM, microscopy.listserver-at-gmail.com wrote:
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} Email: tayl1238-at-vandals.uidaho.edu Name: Martin Taylor
}
} Organization: University of Idaho
}
} Title-Subject: [Filtered] EBSD
}
} Message: Regarding a metallic sample. I read that the sample should have the Normal direction facing
} the EBSD detector (at 70 degrees) for pole figures, but does the normal direction need to be facing
} the detector for Euler, Schmid, Normal, Transverse, and Rolling maps?
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--
Jacob Kabel
Electron Microscopist
Department of Materials Engineering
The University of British Columbia
6350 Stores Road, Room 419
Vancouver, British Columbia
Canada
V6T 1Z4
Phone: (604) 822-5648
Fax: (604) 822-3619
http://emlab.mtrl.ubc.ca/


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From: microscopy.listserver-at-gmail.com
Date: Thu, 3 Aug 2017 17:00:36 -0500
Subject: [Microscopy] viaWWW:EM Lab Director opening

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Organization: Columbia University

Title-Subject: [Filtered] EM Lab Director

Message: The Columbia Nano Initiative (CNI) at The Fu Foundation School of Engineering & Applied
Science of Columbia University in the City of New York invites applications for a Staff Associate or
Senior Staff Associate position, serving as an Electron Microscopy Lab Director.
Reporting to the Shared Facilities Director, the Electron Microscopy lab director will be
responsible for all aspects of the Electron Microscopy Facility, including: implementing safety
regulations and procedures, monitoring the performance of equipment; performing routine maintenance,
calibration, and repairs and coordinating service calls; assisting in the development and upgrade of
instruments including benchmarking and negotiating with vendors; assisting in writing proposals and
raising funds for instrument acquisition and upgrade and other microscopy-related projects and
equipment; preparation of usage forecasts and assistance with strategic planning; supervising lab
assistants.
The EM Lab Director will prepare user training documents; perform training of new users; monitor
usage and maintain user records; assist in teaching classes, modules and short courses that address
the needs of facility users; assist in preparation and teaching of specialized course materials and
Electron Microscopy courses.
The EM director also will provide training, technical assistance, and advice to users on specimen
preparation, operation of instruments and interpretation of data; provide advice and assistance
and/or assign an appropriate subordinate to meet requests of users; operate instruments, obtain and
interpret data in accordance with specific requests from users; provide tours and demonstrations of
the Electron Microscopy Facility; develop a vibrant external users program. Required: Bachelor'ss
degree or higher in an engineering or science field. Minimum of four years' hands-on experience in
operation and/or maintenance of scanning and transmission electron microscopes. Preference given to
candidates with electron microscopy related thesis projects. Application link:
https://academicjobs.columbia.edu/applicants/jsp/shared/frameset/Frameset.jsp?time=1501788707343

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From: zaluzec-at-microscopy.com
Date: Thu, 3 Aug 2017 20:21:23 -0500
Subject: [Microscopy] viaWWW:SEM in Glovebox with ARGON

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Email: miketoalson-at-gmail.com Name: Mike Toalson

Organization: NanoImages, LLC

Title-Subject: [Filtered] SEM in Glovebox with ARGON

Message: Kindly asking the community for some guidance on the use of an SEM or any electronic
instrument for that matter, in a glovebox having an Argon atmosphere.

After hours of extensive searching, I have not located any experience with 2 reported concerns:

1) That there can be issues with arcing of circuit boards or exposed electrical components.

2) That motors are subject to overheating - probably only for open motors that are cooled by the
air, but how would a sealed Turbo Molecular Pump fare?

3) Argon's breakdown voltage is 20% of that of Air. What effect would this have inside an electronic
device like a instrument or SEM?

Thank you so much for any advice!!

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From: microscopy.listserver-at-gmail.com
Date: Thu, 3 Aug 2017 20:22:35 -0500
Subject: [Microscopy] viaWWW:Question to Users of Tungsten Filament SEM

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Email: miketoalson-at-gmail.com Name: Mike Toalson

Organization: NanoImages, LLC

Title-Subject: [Filtered] Question to Users of Tungsten Filament SEM

Message: Please help me formulate a stance on a debate about the effects of an SEM's Tungsten
filament breaking when they fail or "burn out".

One vendor is raising the alarm that if a user waits until a tungsten filament breaks or fails prior
to replacing it, that they run the risk of experiencing a much bigger cleanup issue and even having
"shards" from the filament passing over to the Turbo Pump turbines and causing damage.

I've seen plenty of burned out Tungsten filaments but never experienced these issues. Of course,
the Wehnelt or Anode can often need a good cleaning which is why most labs have some Pikal or Wenol
paste handy.

Other than the interruption to replace a filament, has anybody with a Thermonic Tungsten Filament
SEM ever have a major problem from the filament burning out?

Any thoughts on this matter are much appreciated!!

Mike Toalson
NanoImages, LLC

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From: microscopy.listserver-at-gmail.com
Date: Thu, 3 Aug 2017 20:24:08 -0500
Subject: [Microscopy] viaWWW:SEM in Glovebox with ARGON

Contents Retrieved from Microscopy Listserver Archives
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Organization: NanoImages, LLC

Title-Subject: [Filtered] SEM in Glovebox with ARGON

Message: Kindly asking the community for some guidance on the use of an SEM or any electronic
instrument for that matter, in a glovebox having an Argon atmosphere.

After hours of extensive searching, I have not located any experience with 2 reported concerns:

1) That there can be issues with arcing of circuit boards or exposed electrical components.

2) That motors are subject to overheating - probably only for open motors that are cooled by the
air, but how would a sealed Turbo Molecular Pump fare?

3) Argon's breakdown voltage is 20% of that of Air. What effect would this have inside an electronic
device like a instrument or SEM?

Thank you so much for any advice!!

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Listserver Email Form V - 20120416
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From: vitalylazar-at-att.net
Date: Thu, 3 Aug 2017 21:05:18 -0500
Subject: [Microscopy] Re: viaWWW:SEM in Glovebox with ARGON

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

2 problems:

1) heat transfer - Argon has about 67% of thermal conductivity of air at
normal pressure / T making overheating of electrical components quite
possible. Watch your thermocouple and pirani vacuum gauges for starts.
Forced cooling (as in motors with fans) won't help much because specific
heat of Ar is about half of that of air.

2) breakdown voltage of Argon is 0.2 of air. Circuits with voltages
above low ones (5 / 12 / 24 V) will be at risk.

more points?

Vitaly Feingold
SIA
2773 Heath Lane
Duluth GA 30096
Ph. 770-232-7785
Fax 770-232-1791
www.sia-cam.com
vitaly-at-sia-cam.com

On 8/3/2017 9:26 PM, microscopy.listserver-at-gmail.com wrote:
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}
} Organization: NanoImages, LLC
}
} Title-Subject: [Filtered] SEM in Glovebox with ARGON
}
} Message: Kindly asking the community for some guidance on the use of an SEM or any electronic
} instrument for that matter, in a glovebox having an Argon atmosphere.
}
} After hours of extensive searching, I have not located any experience with 2 reported concerns:
}
} 1) That there can be issues with arcing of circuit boards or exposed electrical components.
}
} 2) That motors are subject to overheating - probably only for open motors that are cooled by the
} air, but how would a sealed Turbo Molecular Pump fare?
}
} 3) Argon's breakdown voltage is 20% of that of Air. What effect would this have inside an electronic
} device like a instrument or SEM?
}
} Thank you so much for any advice!!
}
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From: vitalylazar-at-att.net
Date: Thu, 3 Aug 2017 21:18:49 -0500
Subject: [Microscopy] Re: viaWWW:Question to Users of Tungsten Filament SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Tungsten filament won't cause any problems beyond periodic routine
maintenance (cleaning) of gun components unless electrical malfunction
turns it into a photo-flash all the time - which is extremely unlikely.
Wouldn't worry about that.

Vitaly Feingold
SIA
2773 Heath Lane
Duluth GA 30096
Ph. 770-232-7785
Fax 770-232-1791
www.sia-cam.com
vitaly-at-sia-cam.com

On 8/3/2017 9:24 PM, microscopy.listserver-at-gmail.com wrote:
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}
} Title-Subject: [Filtered] Question to Users of Tungsten Filament SEM
}
} Message: Please help me formulate a stance on a debate about the effects of an SEM's Tungsten
} filament breaking when they fail or "burn out".
}
} One vendor is raising the alarm that if a user waits until a tungsten filament breaks or fails prior
} to replacing it, that they run the risk of experiencing a much bigger cleanup issue and even having
} "shards" from the filament passing over to the Turbo Pump turbines and causing damage.
}
} I've seen plenty of burned out Tungsten filaments but never experienced these issues. Of course,
} the Wehnelt or Anode can often need a good cleaning which is why most labs have some Pikal or Wenol
} paste handy.
}
} Other than the interruption to replace a filament, has anybody with a Thermonic Tungsten Filament
} SEM ever have a major problem from the filament burning out?
}
} Any thoughts on this matter are much appreciated!!
}
} Mike Toalson
} NanoImages, LLC
}
} Login Host: 47.208.204.209
} Listserver Email Form V - 20120416
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From: John.Mardinly-at-asu.edu
Date: Thu, 3 Aug 2017 23:08:37 -0500
Subject: [Microscopy] Re: viaWWW:Question to Users of Tungsten Filament SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Would this vendor by any chance be a vendor of filaments that he wants to sell you? This sounds like a scam. Tungsten evaporates continuously from the filament during its operation. If it is overheated, there can be some melting, but the liquid coalesces into little balls on the ends of the ‘gap’ in the filament. There is nothing that would migrate from the gun to the turbo pump that would cause turbo failure. Pieces of specimen falling off your mounts are the bigger concern.

John Mardinly

}
} Remember this posting is most likely not from a Subscriber, so when replying please copy both
} miketoalson-at-gmail.com as well as the Microscopy Listserver
} ---------------------------------------------------------------------------
}
} Email: miketoalson-at-gmail.com Name: Mike Toalson
}
} Organization: NanoImages, LLC
}
} Title-Subject: [Filtered] Question to Users of Tungsten Filament SEM
}
} Message: Please help me formulate a stance on a debate about the effects of an SEM's Tungsten
} filament breaking when they fail or "burn out".
}
} One vendor is raising the alarm that if a user waits until a tungsten filament breaks or fails prior
} to replacing it, that they run the risk of experiencing a much bigger cleanup issue and even having
} "shards" from the filament passing over to the Turbo Pump turbines and causing damage.
}
} I've seen plenty of burned out Tungsten filaments but never experienced these issues. Of course,
} the Wehnelt or Anode can often need a good cleaning which is why most labs have some Pikal or Wenol
} paste handy.
}
} Other than the interruption to replace a filament, has anybody with a Thermonic Tungsten Filament
} SEM ever have a major problem from the filament burning out?
}
} Any thoughts on this matter are much appreciated!!
}
} Mike Toalson
} NanoImages, LLC
}
}




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From: vitalylazar-at-att.net
Date: Thu, 3 Aug 2017 23:34:13 -0500
Subject: [Microscopy] viaWWW:Question to Users of Tungsten Filament

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

actually way less likely than asteroid hitting the Earth. Asteroids did
that many times. Fragments of EM filament hitting turbo pump? not so
much...

Vitaly Feingold
SIA
2773 Heath Lane
Duluth GA 30096
Ph. 770-232-7785
Fax 770-232-1791
www.sia-cam.com
vitaly-at-sia-cam.com

On 8/4/2017 12:05 AM, Pierre Bustanoby wrote:
} Silly vendor......
} Exploding filaments are always caused by operators applying too much filament heat/current.
} A properly saturated filament slowly thins at the tip as the tungsten sputters away over its life time and as the tip narrows it will take less and less current to saturate. The possibility of an "exploding" filament destroying the turbo is about as likely as an asteroid hitting the earth.
} Pierre Bustanoby
} Specialized
} Electron
} Microscope
} Service
}
} Sent from my iPhone
}
} } On Aug 3, 2017, at 7:37 PM, vitalylazar-at-att.net wrote:
} }
} }
} }
} }
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
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} }
} } Tungsten filament won't cause any problems beyond periodic routine
} } maintenance (cleaning) of gun components unless electrical malfunction
} } turns it into a photo-flash all the time - which is extremely unlikely.
} } Wouldn't worry about that.
} }
} } Vitaly Feingold
} } SIA
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} } } Title-Subject: [Filtered] Question to Users of Tungsten Filament SEM
} } }
} } } Message: Please help me formulate a stance on a debate about the effects of an SEM's Tungsten
} } } filament breaking when they fail or "burn out".
} } }
} } } One vendor is raising the alarm that if a user waits until a tungsten filament breaks or fails prior
} } } to replacing it, that they run the risk of experiencing a much bigger cleanup issue and even having
} } } "shards" from the filament passing over to the Turbo Pump turbines and causing damage.
} } }
} } } I've seen plenty of burned out Tungsten filaments but never experienced these issues. Of course,
} } } the Wehnelt or Anode can often need a good cleaning which is why most labs have some Pikal or Wenol
} } } paste handy.
} } }
} } } Other than the interruption to replace a filament, has anybody with a Thermonic Tungsten Filament
} } } SEM ever have a major problem from the filament burning out?
} } }
} } } Any thoughts on this matter are much appreciated!!
} } }
} } } Mike Toalson
} } } NanoImages, LLC
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} } 4, 33 -- Subject: Re: [Microscopy] viaWWW:Question to Users of Tungsten Filament SEM
} } 4, 33 -- References: {201708040124.v741OkX2003567-at-microscopy.com}
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==============================Original Headers==============================
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4, 33 -- SEM
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From: Ray.Cantrill-at-bluescopesteel.com
Date: Fri, 4 Aug 2017 00:00:25 -0500
Subject: [Microscopy] Re: viaWWW:Question to Users of Tungsten Filament SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have been using Tungsten filaments in a variety of SEM's and an EPMA for over 35 years and I have never heard of this issue being raised before and never experienced such a phenomenon. My feedback would be similar to what John Mardinly has written below.

Regards

Ray Cantrill



-----Original Message-----
X-from: John.Mardinly-at-asu.edu [mailto:John.Mardinly-at-asu.edu]
Sent: Friday, 4 August 2017 2:16 PM
To: Cantrill, Ray K

Would this vendor by any chance be a vendor of filaments that he wants to sell you? This sounds like a scam. Tungsten evaporates continuously from the filament during its operation. If it is overheated, there can be some melting, but the liquid coalesces into little balls on the ends of the ‘gap’ in the filament. There is nothing that would migrate from the gun to the turbo pump that would cause turbo failure. Pieces of specimen falling off your mounts are the bigger concern.

John Mardinly

}
} Remember this posting is most likely not from a Subscriber, so when replying please copy both
} miketoalson-at-gmail.com as well as the Microscopy Listserver
} ---------------------------------------------------------------------------
}
} Email: miketoalson-at-gmail.com Name: Mike Toalson
}
} Organization: NanoImages, LLC
}
} Title-Subject: [Filtered] Question to Users of Tungsten Filament SEM
}
} Message: Please help me formulate a stance on a debate about the effects of an SEM's Tungsten
} filament breaking when they fail or "burn out".
}
} One vendor is raising the alarm that if a user waits until a tungsten filament breaks or fails prior
} to replacing it, that they run the risk of experiencing a much bigger cleanup issue and even having
} "shards" from the filament passing over to the Turbo Pump turbines and causing damage.
}
} I've seen plenty of burned out Tungsten filaments but never experienced these issues. Of course,
} the Wehnelt or Anode can often need a good cleaning which is why most labs have some Pikal or Wenol
} paste handy.
}
} Other than the interruption to replace a filament, has anybody with a Thermonic Tungsten Filament
} SEM ever have a major problem from the filament burning out?
}
} Any thoughts on this matter are much appreciated!!
}
} Mike Toalson
} NanoImages, LLC
}
}




==============================Original Headers==============================
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6, 92 -- Subject: Re: [Microscopy] viaWWW:Question to Users of Tungsten Filament SEM
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23, 39 -- Subject: RE: [Microscopy] Re: viaWWW:Question to Users of Tungsten Filament
23, 39 -- SEM
23, 39 -- Thread-Topic: [Microscopy] Re: viaWWW:Question to Users of Tungsten Filament
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From: microscopy.listserver-at-gmail.com
Date: Fri, 4 Aug 2017 05:54:18 -0500
Subject: [Microscopy] Fwd: Re: viaWWW:SEM in Glovebox with ARGON

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Justin Kraft {kraftpiano-at-gmail.com}

Hi Mike,

There would definitely be problems with high voltages in an argon setting, but the question I’ve got
is why put the SEM in an argon glove box, instead of using a gas-chamber transfer system between the
glovebox and the SEM? The specimen itself is in a vacuum in the SEM, so the environmental
sensitivity shouldn’t be an issue.

Justin A. Kraft
J. Kraft Microscopy Services, Inc.
www.jkraftmicro.com


} On Aug 3, 2017, at 8:33 PM, microscopy.listserver-at-gmail.com wrote:
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} Email: miketoalson-at-gmail.com Name: Mike Toalson
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} Organization: NanoImages, LLC
}
} Title-Subject: [Filtered] SEM in Glovebox with ARGON
}
} Message: Kindly asking the community for some guidance on the use of an SEM or any electronic
} instrument for that matter, in a glovebox having an Argon atmosphere.
}
} After hours of extensive searching, I have not located any experience with 2 reported concerns:
}
} 1) That there can be issues with arcing of circuit boards or exposed electrical components.
}
} 2) That motors are subject to overheating - probably only for open motors that are cooled by the
} air, but how would a sealed Turbo Molecular Pump fare?
}
} 3) Argon's breakdown voltage is 20% of that of Air. What effect would this have inside an electronic
} device like a instrument or SEM?
}
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From: microscopy.listserver-at-gmail.com
Date: Fri, 4 Aug 2017 05:55:00 -0500
Subject: [Microscopy] Fwd: Re: viaWWW:SEM in Glovebox with ARGON

Contents Retrieved from Microscopy Listserver Archives
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X-from: Jerry Biehler {jerry.biehler-at-gmail.com}

This will probably not end well if you try it. Anything HV will not be happy. I don't think the
motors will care too much but the turbo pump will probably need a water cooling loop added.
I would look at getting/building a load lock for the SEM and only having that exposes to the inside
of the glovebox.
-Jerry

} On Aug 3, 2017, at 6:48 PM, microscopy.listserver-at-gmail.com wrote:
}
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} Organization: NanoImages, LLC
}
} Title-Subject: [Filtered] SEM in Glovebox with ARGON
}
} Message: Kindly asking the community for some guidance on the use of an SEM or any electronic
} instrument for that matter, in a glovebox having an Argon atmosphere.
}
} After hours of extensive searching, I have not located any experience with 2 reported concerns:
}
} 1) That there can be issues with arcing of circuit boards or exposed electrical components.
}
} 2) That motors are subject to overheating - probably only for open motors that are cooled by the
} air, but how would a sealed Turbo Molecular Pump fare?
}
} 3) Argon's breakdown voltage is 20% of that of Air. What effect would this have inside an electronic
} device like a instrument or SEM?
}
} Thank you so much for any advice!!
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From: microscopy.listserver-at-gmail.com
Date: Fri, 4 Aug 2017 05:55:31 -0500
Subject: [Microscopy] Fwd: Re: viaWWW:Question to Users of Tungsten Filament

Contents Retrieved from Microscopy Listserver Archives
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X-from: Jerry Biehler {jerry.biehler-at-gmail.com}

Filaments are tiny and they tend to "burn out" because tungsten has evaporated away. Only is
something catastrophic happened would a filament blow with a lot of debris.
None of that debris would ever get to the turbo and even if it did it is so small it would pass
right through.
Your vendor just wants to sell you a lot of consumables.


-Jerry

} On Aug 3, 2017, at 6:48 PM, microscopy.listserver-at-gmail.com wrote:
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}
} Title-Subject: [Filtered] Question to Users of Tungsten Filament SEM
}
} Message: Please help me formulate a stance on a debate about the effects of an SEM's Tungsten
} filament breaking when they fail or "burn out".
}
} One vendor is raising the alarm that if a user waits until a tungsten filament breaks or fails prior
} to replacing it, that they run the risk of experiencing a much bigger cleanup issue and even having
} "shards" from the filament passing over to the Turbo Pump turbines and causing damage.
}
} I've seen plenty of burned out Tungsten filaments but never experienced these issues. Of course,
} the Wehnelt or Anode can often need a good cleaning which is why most labs have some Pikal or Wenol
} paste handy.
}
} Other than the interruption to replace a filament, has anybody with a Thermonic Tungsten Filament
} SEM ever have a major problem from the filament burning out?
}
} Any thoughts on this matter are much appreciated!!
}
} Mike Toalson
} NanoImages, LLC
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From: microscopy.listserver-at-gmail.com
Date: Fri, 4 Aug 2017 05:57:33 -0500
Subject: [Microscopy] viaWWW:FIB-SEM sample preparation of mature bivalve

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X-from: maxim.a.beliaev-at-gmail.com


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Email: maxim.a.beliaev-at-gmail.com Name: Maxim Belyaev

Organization: TU Dresden

Title-Subject: [Filtered] FIB-SEM sample preparation of mature bivalve

Message: What is the best way to prepare bivalve mollusk for the FIB-SEM experiment? We do not want
to cut the tissue before fixation in order to preserve internal structure. So, the thickness of the
tissue can be up to 2 cm. Additional obstacle for the fixation process is that the tissue is
occluded by the shell from one of the sides that we also want to preserve and image. Can prolonged
fixation (for 48 h) in glutaraldehyde in cacodylate buffer and sucrose at 4 degrees C be successful?
Or if chemical fixation of such big sample would not work, can we use snap-freezing or rapid freeze
and freeze-substitution? Can we skip embedding step?


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From: trh-at-uoregon.edu
Date: Fri, 4 Aug 2017 06:33:40 -0500
Subject: [Microscopy] Manufacturers of clean SiN grids in Europe

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Hi all,
I recently started a postdoc in Europe, and I've just learned that
there's some kind of (100 nm-scale) contamination or some other
localized inhomogenieties of varying densities in or on the silicon
nitride sold by a few manufacturers (e.g. TedPella, SIMPore). In my
PhD, I always worked with SPI membranes, which are quite clean.
However, it takes quite a while to fill an order for SPI membranes in
Europe, as they go through a UK reseller who doesn't keep any SPI
products in stock.

I'd like to find a European manufacturer that produces very clean
membranes so that I can quickly send new packs to collaborators when
necessary (and know that any contamination I see isn't my fault). Does
anybody have any experience with any particularly clean membranes that
are made (or at least kept in stock) in Europe? Agar Scientific and
Micro to Nano both carry grids with the membrane thickess and window
size I'm looking for--has anybody noticed any contamination on their
grids?

Thanks,
Tyler

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From: oshel1pe-at-cmich.edu
Date: Fri, 4 Aug 2017 06:34:49 -0500
Subject: [Microscopy] Re: viaWWW:Question to Users of Tungsten Filament SEM

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Would this vendor happen to have any real estate interest in bridges? Maybe the one in Brooklyn?

More seriously, unless the person who is spreading "alternate facts" is the owner of the company, you might check with the company to see if they know he/she is saying this.
I doubt they'd be happy about it.

Philip Oshel
Imaging Core
Central Michigan University

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Email: miketoalson-at-gmail.com Name: Mike Toalson

Organization: NanoImages, LLC

Title-Subject: [Filtered] Question to Users of Tungsten Filament SEM

Message: Please help me formulate a stance on a debate about the effects of an SEM's Tungsten
filament breaking when they fail or "burn out".

One vendor is raising the alarm that if a user waits until a tungsten filament breaks or fails prior
to replacing it, that they run the risk of experiencing a much bigger cleanup issue and even having
"shards" from the filament passing over to the Turbo Pump turbines and causing damage.

I've seen plenty of burned out Tungsten filaments but never experienced these issues. Of course,
the Wehnelt or Anode can often need a good cleaning which is why most labs have some Pikal or Wenol
paste handy.

Other than the interruption to replace a filament, has anybody with a Thermonic Tungsten Filament
SEM ever have a major problem from the filament burning out?

Any thoughts on this matter are much appreciated!!

Mike Toalson
NanoImages, LLC

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From: gilpin-at-purdue.edu
Date: Fri, 4 Aug 2017 14:27:26 -0500
Subject: [Microscopy] Fwd: Re: viaWWW:SEM in Glovebox with ARGON

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

All,
Did I miss the original post as to why this needs to be done? Does there need to be manipulation of the sample in the glove box whilst imaging? Why add an SEM to a glove box?

My thoughts are heading towards using an ESEM with Argon gas in the chamber. There are nano manipulators too

​​​​​
Chris

Christopher J. Gilpin Ph.D.
Director, Life Science Microscopy Facility
Campus-wide Coordinator for Electron Microscopy
Purdue University
Whistler Hall of Agriculture Research, Room S052
170 S. University St
West Lafayette, IN 47907
765-494-7750
gilpin-at-purdue.edu
lsmf-at-purdue.edu reaches everyone in the facility.
http://ag.purdue.edu/arp/Microscopy/Pages/default.aspx


-----Original Message-----
X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com]
Sent: Friday, August 04, 2017 7:18 AM
To: Gilpin, Christopher J {gilpin-at-purdue.edu}

X-from: Jerry Biehler {jerry.biehler-at-gmail.com}

This will probably not end well if you try it. Anything HV will not be happy. I don't think the
motors will care too much but the turbo pump will probably need a water cooling loop added.
I would look at getting/building a load lock for the SEM and only having that exposes to the inside
of the glovebox.
-Jerry

} On Aug 3, 2017, at 6:48 PM, microscopy.listserver-at-gmail.com wrote:
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} Email: miketoalson-at-gmail.com Name: Mike Toalson
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} Organization: NanoImages, LLC
}
} Title-Subject: [Filtered] SEM in Glovebox with ARGON
}
} Message: Kindly asking the community for some guidance on the use of an SEM or any electronic
} instrument for that matter, in a glovebox having an Argon atmosphere.
}
} After hours of extensive searching, I have not located any experience with 2 reported concerns:
}
} 1) That there can be issues with arcing of circuit boards or exposed electrical components.
}
} 2) That motors are subject to overheating - probably only for open motors that are cooled by the
} air, but how would a sealed Turbo Molecular Pump fare?
}
} 3) Argon's breakdown voltage is 20% of that of Air. What effect would this have inside an electronic
} device like a instrument or SEM?
}
} Thank you so much for any advice!!
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From: microscopy.listserver-at-gmail.com
Date: Sun, 6 Aug 2017 17:59:51 -0500
Subject: [Microscopy] viaWWW:Help Requested, HV Issue on Philips 525M SEM

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Email: Tim_Thomas-at-tkd-inc.com Name: Tim Thomas

Organization: PDX Photonics

Title-Subject: [Filtered] Help Requested, HV Issue on Philips 525M SEM

Message: Dear All,

I am the owner of a very nice Philips model 525M SEM. It has a LAB6
source and EDAX. It has been leaking silicon oil from the rubber sealed
box containing capacitors that is attached to the side panel inside the
instrument for many years. The instrument ran fine until recently. The
detector signal started displaying severe noise, then there was a
popping sound. The noise went pop, pop, pop, at about 2 or 3 times a
second coming from under the instrument. I assume the noise is coming
from the HV box under the instrument and not coming from the capacitor
box. I shut the instrument down. The ion pump is still running to keep
the vacuum up near the source. Your guidance, recommendations,
instructions, questions, etc., for helping me bring back to life this
wonderful workhorse of an instrument are appreciated.

One additional detail, there are a few small holes on the exterior of
the metal around the column. These are starting to shown some kind of
foam being extruded from them. The foam is bulging out of the holes by
2 or 3 mm. Not sure if that is significant or not.

Thank you,
Tim Thomas
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From: microscopy.listserver-at-gmail.com
Date: Sun, 6 Aug 2017 18:01:12 -0500
Subject: [Microscopy] viaWWW: Tecnai 12 TEM help.

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Email: book-at-mail.huji.ac.il Name: Naomi Book

Organization: Bio-Imaging Unit, The Alexander Silberman Institute of
Life Science Edmond Safra Campus (G Ram) The Hebrew University
Jerusalem, Israel

Title-Subject: [Filtered] Tecnai 12 TEM
Message: We have a FEI Tecnai 12 TEM that is malfunctioning. We suspect
one of the power supplies that claims to be a Cherokee PE4130 5V 30A
supply, and plugs into a backplane. Cherokee is long gone, and we have
no technical info on the TEM, so we now turn to the list for help.

1. Does anyone who has this unit have a service manual that they can
share with us?

2 .Is there a retired Tecnai 12 out there whose owners would be willing
to provide/sell modules from?

3. Can anyone share experiences (perhaps off-list) concerning
experience with service people who are really good with these instruments?

Thanks in advance.
Naomi Melamed-Book, Ph.D
Bio-Imaging Unit-Director




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From: microscopy.listserver-at-gmail.com
Date: Sun, 6 Aug 2017 18:07:25 -0500
Subject: [Microscopy] viaWWW:Question to Users of Tungsten Filament SEM

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X-from: Smith, III, Julian P.S {smithj-at-winthrop.edu}


?? I've never heard of this. Certainly, my JEOL service engineer
doesn't recommend anything but replacing the filament after it fails,
and our 'scope has a turbopump.

As for "shards"--I doubt it, but don't know.

As for "more cleanup"--well yes, I suppose in that final "bye, cruel
world" flash, you get more deposited tungsten. But you're going to
clean the wehnelt and anode anyway at each filament change.

Julian

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*Sent:* Thursday, August 3, 2017 9:35:39 PM
*To:* Smith, III, Julian P.S
*Subject:* [Microscopy] viaWWW:Question to Users of Tungsten Filament SEM



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Email: miketoalson-at-gmail.com Name: Mike Toalson

Organization: NanoImages, LLC

Title-Subject: [Filtered] Question to Users of Tungsten Filament SEM

Message: Please help me formulate a stance on a debate about the effects
of an SEM's Tungsten
filament breaking when they fail or "burn out".

One vendor is raising the alarm that if a user waits until a tungsten
filament breaks or fails prior
to replacing it, that they run the risk of experiencing a much bigger
cleanup issue and even having
"shards" from the filament passing over to the Turbo Pump turbines and
causing damage.

I've seen plenty of burned out Tungsten filaments but never experienced
these issues. Of course,
the Wehnelt or Anode can often need a good cleaning which is why most
labs have some Pikal or Wenol
paste handy.

Other than the interruption to replace a filament, has anybody with a
Thermonic Tungsten Filament
SEM ever have a major problem from the filament burning out?

Any thoughts on this matter are much appreciated!!

Mike Toalson
NanoImages, LLC

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From: microscopy.listserver-at-gmail.com
Date: Sun, 6 Aug 2017 18:08:27 -0500
Subject: [Microscopy] viaWWW:SEM in Glovebox with ARGON

Contents Retrieved from Microscopy Listserver Archives
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X-from: Matt Jobbins {mmjobbins-at-gmail.com}


It is absolutely correct that putting a microscope in an argon glove box
is generally a bad idea due to the low dielectric breakdown voltage of
argon. It is, however, of enough interest that multiple vendors of
desktop SEMs have investigated it. For instance:
https://www.phenom-world.com/accessories/phenom-series/glove-box-compatibility-kit

There are a couple of benefits in addition to reduced complexity
associated with multiple load locks and purge chambers.

Argon has a higher pumping speed than house air resulting in reduced
pressures and nominally improved service lifetimes of many components.
The complete lack of water in the chamber, in principle, allows for the
window protecting an SDD (from condensed water) to be removed making
lithium detectable by EDS.

-Matt



On Fri, Aug 4, 2017 at 7:26 AM, {microscopy.listserver-at-gmail.com
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X-from: Jerry Biehler {jerry.biehler-at-gmail.com
{mailto:jerry.biehler-at-gmail.com} }

This will probably not end well if you try it. Anything HV will not
be happy. I don't think the
motors will care too much but the turbo pump will probably need a
water cooling loop added.
I would look at getting/building a load lock for the SEM and only
having that exposes to the inside
of the glovebox.
-Jerry

} On Aug 3, 2017, at 6:48 PM, microscopy.listserver-at-gmail.com
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} Email: miketoalson-at-gmail.com {mailto:miketoalson-at-gmail.com} Name:
Mike Toalson
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} Organization: NanoImages, LLC
}
} Title-Subject: [Filtered] SEM in Glovebox with ARGON
}
} Message: Kindly asking the community for some guidance on the use
of an SEM or any electronic
} instrument for that matter, in a glovebox having an Argon
atmosphere.
}
} After hours of extensive searching, I have not located any
experience with 2 reported concerns:
}
} 1) That there can be issues with arcing of circuit boards or
exposed electrical components.
}
} 2) That motors are subject to overheating - probably only for
open motors that are cooled by the
} air, but how would a sealed Turbo Molecular Pump fare?
}
} 3) Argon's breakdown voltage is 20% of that of Air. What effect
would this have inside an electronic
} device like a instrument or SEM?
}
} Thank you so much for any advice!!
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From: diller-at-stefan-diller.com
Date: Mon, 7 Aug 2017 04:14:48 -0500
Subject: [Microscopy] Re: viaWWW:Help Requested, HV Issue on Philips 525M SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Tim,

the leaking of silicon-oil in older versions of the high voltage parts (like gun, HV main supply and the side-mounted HV box to
deliver the HV needed for the cathode tubes for viewing / recording) is a well-known issue at 5x5 electronics.

It even happens at the HV cascade to produce the lower voltages needed for the SE detector cage (used in the SE module on the
operator console).

The best you can do is looking for a 5x5 SEM to break down for parts and hope you get a newer (black, not white) version of
silicon used in those parts.

If your monitors are still working showing an undisturbed image, the box sitting left-side back down in the electronics might
still be OK.

If at small acceleration voltages (ca. 5kV) you get a stable image and the image gets noisy going up in voltage (happens mostly at
20 to 30 kV) your main HV supply (shoebox-size...) is shortening and you need a replacement.

You can try clean the cascades from silicon residue and use a new high-voltage isolating silicon to newly isolate the parts but if
there had been a lot of discharging happening in the past in the cascades you might have burned a carbon layer which makes it
impossible to use / refurbish these parts.

The "foam" you mentioned is coming from the upper part of the column (containing the cathode assembly) ? There is also silicon
used for the heating transformer isolation. If it`s the old version (whitish) it will come out with times... You can only try
cleaning the holes with Peth-Ether / Acetone and glue them shut two-component resin. The problem will get worse, since you need to
tilt the gun 90 when changing the filament (or you dismount the upper column part and keep it upright all the times during
filament change...).

...

The best way would be to look around for parts and exchange the faulty ones. Ask here at the listserver...

If you don`t find parts near you, come back to me and I look around in Germany.


Best wishes,

Stefan


-----------------------------------------------------
Stefan Diller - Scientific Photography
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D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
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Websites:
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Am 07.08.17 um 01:33 schrieb microscopy.listserver-at-gmail.com:
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} Title-Subject: [Filtered] Help Requested, HV Issue on Philips 525M SEM
}
} Message: Dear All,
}
} I am the owner of a very nice Philips model 525M SEM. It has a LAB6
} source and EDAX. It has been leaking silicon oil from the rubber sealed
} box containing capacitors that is attached to the side panel inside the
} instrument for many years. The instrument ran fine until recently. The
} detector signal started displaying severe noise, then there was a
} popping sound. The noise went pop, pop, pop, at about 2 or 3 times a
} second coming from under the instrument. I assume the noise is coming
} from the HV box under the instrument and not coming from the capacitor
} box. I shut the instrument down. The ion pump is still running to keep
} the vacuum up near the source. Your guidance, recommendations,
} instructions, questions, etc., for helping me bring back to life this
} wonderful workhorse of an instrument are appreciated.
}
} One additional detail, there are a few small holes on the exterior of
} the metal around the column. These are starting to shown some kind of
} foam being extruded from them. The foam is bulging out of the holes by
} 2 or 3 mm. Not sure if that is significant or not.
}
} Thank you,
} Tim Thomas
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From: microscopy.listserver-at-gmail.com
Date: Mon, 7 Aug 2017 06:41:45 -0500
Subject: [Microscopy] viaWWW:SEM in Glovebox with ARGON

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Mike Toalson {miketoalson-at-gmail.com}



Thank you so much to all in the community for providing some great
comments and suggestions.

Some have asked why was this being asked about? Our company is a
distributor of tabletop SEM and we have a project where a customer with
another type SEM wishes to have a system inside an argon filled glovebox
for analyzing samples that cannot be exposed to air.Like many of you, my
first thought was “why not use a transfer device like the Quorum
accessory?”.However the type of SEM being considered (not ours) has no
external port so the solution for them is to put the entire compact SEM
in the glovebox with a special modification kit.

Our system has 2 “boxes” with extendable cables to the electronics box
so only the column and HV supply would be in the glovebox. Thus,I was
curious about experiences with this.I do like the one idea of using a
cold plate to keep the components from overheating.More study is needed
though and the use of a transfer system seems less complicated for sure
however, our system only has one side port that is normally used for an
EDS but we are looking into modifications.

Curious if any of you have looked at using something like the small
transfer device shown here with the “rupture” film?My first concern
would be does the film stay intact after splitting open but the paper
describes using elastic films that are not prone to this.It seems like a
simple and elegant way to move a sample from glovebox to SEM without the
need of an Airlock or complex shuttle.

www.creol.ucf.edu/research/publications/5296.pdf
{http://www.creol.ucf.edu/research/publications/5296.pdf}

Thank You!!

Mike Toalson

NanoImages, LLC

t •866.601.6266

m•530.691.3180

e •mtoalson-at-nanoimages.com

web•www.nanoimages.com {http://www.nanoimages.com/}

skype •mike.toalson


On Sun, Aug 6, 2017 at 4:38 PM, {microscopy.listserver-at-gmail.com
{mailto:microscopy.listserver-at-gmail.com} } wrote:





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X-from: Matt Jobbins {mmjobbins-at-gmail.com
{mailto:mmjobbins-at-gmail.com} }


It is absolutely correct that putting a microscope in an argon
glove box
is generally a bad idea due to the low dielectric breakdown voltage of
argon. It is, however, of enough interest that multiple vendors of
desktop SEMs have investigated it. For instance:

https://www.phenom-world.com/accessories/phenom-series/glove-box-compatibility-kit

{https://www.phenom-world.com/accessories/phenom-series/glove-box-compatibility-kit}

There are a couple of benefits in addition to reduced complexity
associated with multiple load locks and purge chambers.

Argon has a higher pumping speed than house air resulting in reduced
pressures and nominally improved service lifetimes of many components.
The complete lack of water in the chamber, in principle, allows for the
window protecting an SDD (from condensed water) to be removed making
lithium detectable by EDS.

-Matt



On Fri, Aug 4, 2017 at 7:26 AM, {microscopy.listserver-at-gmail.com
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X-from: Jerry Biehler {jerry.biehler-at-gmail.com
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This will probably not end well if you try it. Anything HV
will not
be happy. I don't think the
motors will care too much but the turbo pump will probably need a
water cooling loop added.
I would look at getting/building a load lock for the SEM and only
having that exposes to the inside
of the glovebox.
-Jerry

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} Organization: NanoImages, LLC
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} Title-Subject: [Filtered] SEM in Glovebox with ARGON
}
} Message: Kindly asking the community for some guidance on
the use
of an SEM or any electronic
} instrument for that matter, in a glovebox having an Argon
atmosphere.
}
} After hours of extensive searching, I have not located any
experience with 2 reported concerns:
}
} 1) That there can be issues with arcing of circuit boards or
exposed electrical components.
}
} 2) That motors are subject to overheating - probably only for
open motors that are cooled by the
} air, but how would a sealed Turbo Molecular Pump fare?
}
} 3) Argon's breakdown voltage is 20% of that of Air. What
effect
would this have inside an electronic
} device like a instrument or SEM?
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From: microscopy.listserver-at-gmail.com
Date: Mon, 7 Aug 2017 06:42:32 -0500
Subject: [Microscopy] Re: viaWWW:Question to Users of Tungsten Filament SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Kathleen Pullin {kleopullin-at-email.arizona.edu}



I might get a different vendor.

That said, I have heard of and seen this. While visiting a lab running a
TEM, I was shown a filament with the top blown off and told it happened
because there had been a high voltage surge to the instrument, and
tungsten filament debris was found in the scope. TEM, not SEM. I had
just talked about this with a lab mate the might before this question
was posted on the list server.

Never otherwise heard of it.

KP





On Sunday, August 6, 2017, {microscopy.listserver-at-gmail.com
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X-from: Smith, III, Julian P.S {smithj-at-winthrop.edu
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?? I've never heard of this. Certainly, my JEOL service engineer
doesn't recommend anything but replacing the filament after it fails,
and our 'scope has a turbopump.

As for "shards"--I doubt it, but don't know.

As for "more cleanup"--well yes, I suppose in that final "bye, cruel
world" flash, you get more deposited tungsten. But you're going to
clean the wehnelt and anode anyway at each filament change.

Julian


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Filament SEM




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Email: miketoalson-at-gmail.com {javascript:;} Name: Mike Toalson

Organization: NanoImages, LLC

Title-Subject: [Filtered] Question to Users of Tungsten Filament SEM

Message: Please help me formulate a stance on a debate about the
effects
of an SEM's Tungsten
filament breaking when they fail or "burn out".

One vendor is raising the alarm that if a user waits until a tungsten
filament breaks or fails prior
to replacing it, that they run the risk of experiencing a much bigger
cleanup issue and even having
"shards" from the filament passing over to the Turbo Pump turbines and
causing damage.

I've seen plenty of burned out Tungsten filaments but never experienced
these issues. Of course,
the Wehnelt or Anode can often need a good cleaning which is why most
labs have some Pikal or Wenol
paste handy.

Other than the interruption to replace a filament, has anybody with a
Thermonic Tungsten Filament
SEM ever have a major problem from the filament burning out?

Any thoughts on this matter are much appreciated!!

Mike Toalson
NanoImages, LLC

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--
Kleo Pullin
kleopullin-at-email.arizona.edu {mailto:kleopullin-at-email.arizona.edu}
Moraga, CA

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From: microscopy.listserver-at-gmail.com
Date: Fri, 11 Aug 2017 06:27:23 -0500
Subject: [Microscopy] viaWWW:TEM cross-sectional glue

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Mike and company -

Here are two other alternatives to a SEM in a glove-box:


http://www.int.kit.edu/downloads/INT_Research/Flyervacushut.pdf

and

https://www.kammrath-weiss.com/en/products/materials/transfer-module.html

No financial interest.

Looking forward to those OoO messages.

regards,
- Jim

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From jeverett397-at-gmail.com Tue Aug 8 16:49:17 2017
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Email: annett.thogersen-at-sintef.no Name: Annett Thgersen

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Title-Subject: [Filtered] TEM cross-sectional glue

Message: Dear Colleagues,

I am looking for a TEM cross-sectional glue that do not need any heat
for hardening, is easy to mix, and will have minimal contamination in
the TEM. I have one that I use now, but unfortunately it softens in
acetone. Does anyone have some good ideas on what glue I can use? I
cannot expose my samples to very much heat.
Thank you,
Annett Thgersen

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From: microscopy.listserver-at-gmail.com
Date: Fri, 11 Aug 2017 06:28:12 -0500
Subject: [Microscopy] viaWWW:Microscopy Career Opportunities

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From: microscopy.listserver-at-gmail.com
Date: Fri, 11 Aug 2017 06:28:44 -0500
Subject: [Microscopy] viaWWW:Electron Beam Brightness issue Jeol JEM-1010 after filament

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Email: mj_iqbal-at-yahoo Name: M Javed Iqbal

Organization: NIBGE
Title-Subject: [Filtered] Electron Beam Brightness issue Jeol JEM-1010
after filament change, suggestions required

Message: Hello Dear all
suggestions/ help is required regarding the electron microscope filament
beam which is not sharp/bright enough (shadow of the fixed aperture)
after I replaced the fused filament with new one, in past we have done
this practice multiple times without any issue. I have done all the
possible filament distance adjusting options with the wehnelt cap/cover
but no major difference in results more over obtaining filament
saturation point did not yield a converged single very sharp point and
when we De-saturate the filament by turning the filament knob counter
clockwise its not producing the typical image of two balanced half
crescents holing a round spot inside.without engaging any lens aperture
the shadow of fixed aperture in having a hallow zone like double spot
with slight missing the full overlap
Is it possible to post an image or video clip of the same for better
understanding ?

Muhammad javed Iqbal TEM Core Facilities Lab
NIBGE Pakistan

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From: microscopy.listserver-at-gmail.com
Date: Fri, 11 Aug 2017 06:29:31 -0500
Subject: [Microscopy] viaWWW:3 year postdoctoral position available University of Limerick,

Contents Retrieved from Microscopy Listserver Archives
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Email: andy.stewart-at-ul.ie Name: Andy Stewart

Organization: University of Limerick

Title-Subject: [Filtered] 3 year postdoctoral position available

Message: Postdoctoral position in electron microscopy of pharmaceutical
crystals.
A 3-year postdoctoral position in TEM is available at the University of
Limerick, Ireland, as part of the H2020 award MagnaPharm
(https://www.magnapharm.com) the aim of this researchproject is to
control polymorphism in pharmaceutical crystal growth via magnetic
fields, and to solve crystal structure and morphology of these compounds
using electron imaging and diffraction methods as well as to build a
matrix of machine conditions for data evaluation on organic crystals.

Work will be carried out using a double corrected, monochromated Titan
Themis, equipped with EDS and EELS, K2-IS and Oneview detectors, as well
as in-situ holders (DENS lightning double tilt , DENS ocean, DENS
atmosphere, Fischione cryo-transfer holder.)
Please apply here:
http://www.ul.ie
Vacancies
Job ID: 023368

Closing date is the 31th of August 2017.
For further information please contact Ursel.Bangert-at-ul.ie or
Andy.Stewart-at-ul.ie


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From: microscopy.listserver-at-gmail.com
Date: Fri, 11 Aug 2017 06:30:16 -0500
Subject: [Microscopy] viaWWW:October Biological TEM workshop

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Email: jshields-at-uga.edu Name: John P Shields

Organization: University of Georgia

Title-Subject: [Filtered] October Biological TEM workshop

Message: Biological TEM Workshop

This intensive, three-day workshop will provide a practical and basic
theoretical introduction to the Transmission Electron Microscope and
biological sample preparation techniques. Each day will consist of
lecture, discussion and hands-on training led by GEM staff.
What: Anyone requiring training on TEM and biological sample
preparation. The workshop will be limited to 6 participants based on the
availability of equipment.
When: Monday through Wednesday, October 25-27, 2017, 8am-5pm each day
(lunch is provided)

Where: 154 Barrow Hall, University of Georgia, Athens, GA 30602

Registration: Contact John Shields (jpshield-at-uga.edu) for more
information and to sign up. Registration requires iLab account through
the GEM website. https://uga.ilabsolutions.com/account/login

Deadline: October 15, 2017


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From: John.Mardinly-at-asu.edu
Date: Fri, 11 Aug 2017 10:57:37 -0500
Subject: [Microscopy] Re: viaWWW:TEM cross-sectional glue

Contents Retrieved from Microscopy Listserver Archives
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There is an epoxy designed for plugging leaks inUHV systems called “Torr Seal” that is incredible. The only drawback is that it is quite viscous and does not form thin joints. However, it is filled with ceramic particles so it does not mill preferentially. It cures at room temperature, has very strong adhesion and does not contaminate. According to web, still manufactured by Varian and available through Kurt Lesker and others.

John Mardinly


} On Aug 11, 2017, at 4:48 AM, microscopy.listserver-at-gmail.com wrote:
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} Email: annett.thogersen-at-sintef.no Name: Annett Thøgersen
}
} Organization: SINTEF
}
} Title-Subject: [Filtered] TEM cross-sectional glue
}
} Message: Dear Colleagues,
}
} I am looking for a TEM cross-sectional glue that do not need any heat
} for hardening, is easy to mix, and will have minimal contamination in
} the TEM. I have one that I use now, but unfortunately it softens in
} acetone. Does anyone have some good ideas on what glue I can use? I
} cannot expose my samples to very much heat.
} Thank you,
} Annett Thøgersen
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From: microscopy.listserver-at-gmail.com
Date: Fri, 11 Aug 2017 13:17:40 -0500
Subject: [Microscopy] Re: viaWWW:Electron Beam Brightness issue Jeol JEM-1010

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Ribardire Michel {m.ribardiere-at-jeol.fr}



Th main thing is to adjust gun tilt to get Good beam image mission With filament at 100kv must be
around 20uA add to 66uA of HT

Filament knob around 9 o'clock With bias adjusted around 5 to 7
Adjust gun tilt x and y to get max brightness

Regards
Michel

Envoy depuis mon Sony Xperia T3 d'Orange



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Email: mj_iqbal-at-yahoo Name: M Javed Iqbal

Organization: NIBGE
Title-Subject: [Filtered] Electron Beam Brightness issue Jeol JEM-1010
after filament change, suggestions required

Message: Hello Dear all
suggestions/ help is required regarding the electron microscope filament
beam which is not sharp/bright enough (shadow of the fixed aperture)
after I replaced the fused filament with new one, in past we have done
this practice multiple times without any issue. I have done all the
possible filament distance adjusting options with the wehnelt cap/cover
but no major difference in results more over obtaining filament
saturation point did not yield a converged single very sharp point and
when we De-saturate the filament by turning the filament knob counter
clockwise its not producing the typical image of two balanced half
crescents holing a round spot inside.without engaging any lens aperture
the shadow of fixed aperture in having a hallow zone like double spot
with slight missing the full overlap
Is it possible to post an image or video clip of the same for better
understanding ?

Muhammad javed Iqbal TEM Core Facilities Lab
NIBGE Pakistan

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From: microscopy.listserver-at-gmail.com
Date: Fri, 11 Aug 2017 13:18:25 -0500
Subject: [Microscopy] Re: viaWWW:TEM cross-sectional glue

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X-from: Gary Brown {microscopy.gmb-at-gmail.com}


Annett,

I am now retired from ExxonMobil Chemical Company after working 33 in
a polymer microscopy lab. I embedded and sectioned samples for
TEM/STEM over the last 25 years.

I found that EpoFix, from Ted Pella did a great job for room
temperature and heat curing
(https://www.emsdiasum.com/microscopy/products/embedding/kits.aspx)

My notes on the use of EpoFix are given below in italics: The issues
regarding phase separation of the epoxy-curative mixtures early during
the curing process are very real and, if ignored, generally caused
significant problems with curing at room temperature. The solution
that I developed worked every time when applied correctly.


Polymer samples (films, fabrics, fibers, etc.) are embedded at room
temperature or 50C in EpoFix epoxy resin with accelerator (25:3 wt/wt
resin:accelerator) according to the manufacturer's directions.
(EpoFix is available from Electron Microscopy Sciences although Ted
Pella and others may sell a similar resin.)


It is very important to note that the resin-accelerator mixture cures
in about 90 minutes but the two phases separate upon standing during
the initial 45 minutes or so of curing. To prevent this, the mixture
must be manually stirred using a stirring rod, wooden splint, or
equivalent for at least two minutes (timed) and continued stirring
using a magnetic stir bar/stir plate until the resin-accelerator
mixture is stable after 45 to 60 minutes curing; about 60 to 90
minutes, the viscosity of the curing resin gradually increases beyond
a usable point.


The resin-accelerator mixture may be cured at 50C for several hours or
at room temperature overnight. Blocks cured at ambient temperature
may be a little soft after curing overnight; an additional day or more
of curing improves cutting performance. Heat curing is preferred if
the sample can tolerate 50C temperature without annealing or melting.
For example, high density polyethylenes and polypropylenes (melting
temperatures ~130C and 155-160C, respectively) were cured at 50C but
softer; lower density polyethylenes and elastomers containing low
melting polymer fractions that melt or reorganize at or just above
room temperature may change the morphology even at these curing
temperatures.

For use as a glue, I would cure the EpoFix at room temperature until
it is the viscosity you want for a glue.

I hope this helps. I found it to work under all types of polymer samples.

Feel free to contact me with any questions or comments.

Cheers,

Gary M Brown
Polymer Microscopist

On Fri, Aug 11, 2017 at 6:55 AM, {microscopy.listserver-at-gmail.com} wrote:
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} Title-Subject: [Filtered] TEM cross-sectional glue
}
} Message: Dear Colleagues,
}
} I am looking for a TEM cross-sectional glue that do not need any heat
} for hardening, is easy to mix, and will have minimal contamination in
} the TEM. I have one that I use now, but unfortunately it softens in
} acetone. Does anyone have some good ideas on what glue I can use? I
} cannot expose my samples to very much heat.
} Thank you,
} Annett Thøgersen
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--

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in a very narrow field.” and "Prediction is very difficult, especially
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From: microscopy.listserver-at-gmail.com
Date: Fri, 11 Aug 2017 13:19:12 -0500
Subject: [Microscopy] Re: viaWWW:TEM cross-sectional glue

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X-from: Debangshu Mukherjee {debangshu-at-psu.edu}



Hi Annett,
I have used MBond 610 which has to be stored in a refrigerator and sets without any heat in a few
hours. It is pretty resistant to acetone.

Debangshu Mukherjee
PhD Candidate
MatSE, Penn State

On Aug 11, 2017 07:52, {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote:




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Organization: SINTEF

Title-Subject: [Filtered] TEM cross-sectional glue

Message: Dear Colleagues,

I am looking for a TEM cross-sectional glue that do not need any heat
for hardening, is easy to mix, and will have minimal contamination in
the TEM. I have one that I use now, but unfortunately it softens in
acetone. Does anyone have some good ideas on what glue I can use? I
cannot expose my samples to very much heat.
Thank you,
Annett Thøgersen

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From: microscopy.listserver-at-gmail.com
Date: Sun, 13 Aug 2017 08:36:05 -0500
Subject: [Microscopy] viaWWW:Question to Users of Tungsten Filament SEM

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Email: Bilinmadison-at-yahoo.com Name: Bil Schneider

Organization: UW Madison

Title-Subject: [Filtered] Chamberscope

Message: Our KE GW 25 Infrared Chamberscope died. Looking at options to replace it. Does anyone have
suggestions for acquiring a usable GW 25? It fits onto a Hitachi S3400 SEM, or, has anyone upgraded
their Chamberscope and could recommend a reliable replacement?

Thanks,
Bil Schneider
UW Geosciences

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From "www."-at-galaxy.ocn.ne.jp Fri Aug 11 20:43:52 2017
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Reply-To: {Presidency.offiice-at-presidency.com}

X-from: Steve Chapman {protrain-at-emcourses.com}

Hi
I see your problem which you described very clearly. The error that you are
making is due to making a standard error when aligning an instrument. When
we start learning to operate a TEM we worry about the gun alignment and we
often make our own problems. The tendency is to see a spot of light and to
work on that spot of light, but if it does not respond correctly, this is a
shadow! You need to forget the spot of light that does not behave correctly
and find the true beam which will behave correctly; your gun is out of
alignment when it displays this shadow.

Use your gun alignment shift to find the beam and then gun alignment tilt to
bring up the spot and halo that you are familiar with. I suggest you reset
all of your gun alignment controls and start again.
Good luck

Steve

Steve Chapman FRMS
Retired from Protrain for Consultancy and Training in Electron Microscopy
+44 (0)7711 606967
web www.emcourses.com


-----Original Message-----
X-from: microscopy.listserver-at-gmail.com
[mailto:microscopy.listserver-at-gmail.com] Sent: 11 August 2017 19:19
To: protrain-at-emcourses.com

X-from: Ribardire Michel {m.ribardiere-at-jeol.fr}



Th main thing is to adjust gun tilt to get Good beam image mission With
filament at 100kv must be around 20uA add to 66uA of HT

Filament knob around 9 o'clock With bias adjusted around 5 to 7 Adjust gun
tilt x and y to get max brightness

Regards
Michel

Envoy depuis mon Sony Xperia T3 d'Orange



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Email: mj_iqbal-at-yahoo Name: M Javed Iqbal

Organization: NIBGE
Title-Subject: [Filtered] Electron Beam Brightness issue Jeol JEM-1010 after
filament change, suggestions required

Message: Hello Dear all
suggestions/ help is required regarding the electron microscope filament
beam which is not sharp/bright enough (shadow of the fixed aperture) after I
replaced the fused filament with new one, in past we have done this practice
multiple times without any issue. I have done all the possible filament
distance adjusting options with the wehnelt cap/cover but no major
difference in results more over obtaining filament saturation point did not
yield a converged single very sharp point and when we De-saturate the
filament by turning the filament knob counter clockwise its not producing
the typical image of two balanced half crescents holing a round spot
inside.without engaging any lens aperture the shadow of fixed aperture in
having a hallow zone like double spot with slight missing the full overlap
Is it possible to post an image or video clip of the same for better
understanding ?

Muhammad javed Iqbal TEM Core Facilities Lab NIBGE Pakistan

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From janiceking866-at-gmail.com Sat Aug 12 16:34:06 2017
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Reply-To: "Perry Fox" {janiceking866-at-gmail.com}

X-from: Michael Nesta {mnesta.ebs-at-gmail.com}



Mike,

In my 17 years with EBS, I've never seen or heard of a situation where the failure of one of our
Tungsten FIlaments has had a catasrophic effect on a Customer's SEM or TEM. This is not say it is
impossible, but given the hundreds of thousands of FIlaments we have manufactured during my tenure,
one would think that if it were truly a problem that users need to be concerned about, we would have
heard about it happening once or twice...

Sincerely,
Mike Nesta
President
Energy Beam Sciences, Inc.
www.ebsciences.com {http://www.ebsciences.com}

On Thu, Aug 3, 2017 at 9:51 PM, {microscopy.listserver-at-gmail.com
{mailto:microscopy.listserver-at-gmail.com} } wrote:




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Email: miketoalson-at-gmail.com {mailto:miketoalson-at-gmail.com} Name: Mike Toalson

Organization: NanoImages, LLC

Title-Subject: [Filtered] Question to Users of Tungsten Filament SEM

Message: Please help me formulate a stance on a debate about the effects of an SEM's Tungsten
filament breaking when they fail or "burn out".

One vendor is raising the alarm that if a user waits until a tungsten filament breaks or fails
prior
to replacing it, that they run the risk of experiencing a much bigger cleanup issue and even having
"shards" from the filament passing over to the Turbo Pump turbines and causing damage.

I've seen plenty of burned out Tungsten filaments but never experienced these issues. Of course,
the Wehnelt or Anode can often need a good cleaning which is why most labs have some Pikal or Wenol
paste handy.

Other than the interruption to replace a filament, has anybody with a Thermonic Tungsten Filament
SEM ever have a major problem from the filament burning out?

Any thoughts on this matter are much appreciated!!

Mike Toalson
NanoImages, LLC

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From: Philip.Koeck-at-ki.se
Date: Tue, 15 Aug 2017 05:58:03 -0500
Subject: [Microscopy] [TEM] amplitude contrast

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Title-Subject: [Filtered] Cryo Stage

Message: Our laboratory is in the market for a stage with both heat and cryo capabilities for our
Leica Axiophot Light Microscope. Does anyone have recommendations for stage or stage system? The
system I worked on years ago was a dedicated microscope with a system specifically for that purpose.
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From johnsonloretta061yilke-at-gmail.com Sun Aug 13 18:26:21 2017
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Dear all.

Is there any agreement on the cause of amplitude contrast when imaging proteins in cryo-TEM?

Is it the second order term in the expansion of a weak phase object or is it due to inelastic scattering or the aperture or something else?

All the best.

Philip


==============================Original Headers==============================
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From: steven.spurgeon-at-pnnl.gov
Date: Tue, 15 Aug 2017 12:33:14 -0500
Subject: [Microscopy] Data generation trends in microscopy

Contents Retrieved from Microscopy Listserver Archives
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Hello everyone,

I’m looking for a good reference the describes the rapid increase in data being generated in the microscopy field. With the advent of direct detection, we’re now generating vast amounts of data that require new approaches to handle them. I’m wondering, is there an article that describes these trends over time?

Thank you!

______________________________________
Steven R. Spurgeon, Ph.D.
Postdoctoral Research Associate
Physical and Computational Sciences Directorate

Pacific Northwest National Laboratory
902 Battelle Boulevard
P.O. Box 999 MSIN:K8-87
Richland, WA 99352

Tel: +1-509-371-7123
steven.spurgeon-at-pnnl.gov
www.stevenspurgeon.com {http://www.stevenspurgeon.com/}
www.pnnl.gov {http://www.pnnl.gov/}




==============================Original Headers==============================
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From: sergei2-at-ornl.gov
Date: Tue, 15 Aug 2017 13:02:56 -0500
Subject: [Microscopy] Re: Data generation trends in microscopy

Contents Retrieved from Microscopy Listserver Archives
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This would indeed be a very timely topic. For scanning probe microscopy,
there are papers:
Big, Deep, and Smart Data in Scanning Probe Microscopy, ACS Nano, 2016,
10 (10), pp 90689086
and
Big data and deep data in scanning and electron microscopies: deriving
functionality from multidimensional data setsin Advances for Structural
and Chemical imaging, 2015 1:6

There are also some DOE documents, for example

https://science.energy.gov/~/media/ascr/pdf/programdocuments/docs/ascr-eod-workshop-2015-report_160524.pdf

That partially address data generation in physical sciences including
electron microscopy.

Sergei

--
Sergei V. Kalinin
Director, Institute for Functional Imaging of Materials
Fellow MRS, APS, AVS
Oak Ridge National Laboratory
Phone: (865) 241-0236


On 8/15/2017 1:33 PM, steven.spurgeon-at-pnnl.gov wrote:
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} Hello everyone,
}
} I’m looking for a good reference the describes the rapid increase in data being generated in the microscopy field. With the advent of direct detection, we’re now generating vast amounts of data that require new approaches to handle them. I’m wondering, is there an article that describes these trends over time?
}
} Thank you!
}
} ______________________________________
} Steven R. Spurgeon, Ph.D.
} Postdoctoral Research Associate
} Physical and Computational Sciences Directorate
}
} Pacific Northwest National Laboratory
} 902 Battelle Boulevard
} P.O. Box 999 MSIN:K8-87
} Richland, WA 99352
}
} Tel: +1-509-371-7123
} steven.spurgeon-at-pnnl.gov
} www.stevenspurgeon.com {http://www.stevenspurgeon.com/}
} www.pnnl.gov {http://www.pnnl.gov/}
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--
Sergei V. Kalinin

Director,
Institute for Functional Imaging of Materials

Fellow MRS, APS, AVS

Oak Ridge National Laboratory

Phone: (865) 241-0236


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From: microscopy.listserver-at-gmail.com
Date: Wed, 16 Aug 2017 06:42:12 -0500
Subject: [Microscopy] viaWWW:Has anyone tried Sure Cut Surfactant?

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Email: Buchsmith-at-gmail.com Name: JoAnn Buchanan

Organization: Allen Institute for Brain Science

Title-Subject: [Filtered] Has anyone tried Sure Cut Surfactant?

Message: Our lab is cutting large numbers of serial sections. We are having trouble with debris
building up on the diamond knife edge so that we need to stop the microtome and clean it. This is
problematic for several reasons.
I am wondering if anyone has tried Sure Cut added to the resin to improve sectioning?
We are embedding in Embed 812 or Hard Plus resin.
Thanks
JoAnn Buchanan

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From: nicholas.conoan-at-unmc.edu
Date: Thu, 17 Aug 2017 10:47:01 -0500
Subject: [Microscopy] Preservation of microtubules

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello All:

Our facility has received a project inquiry in which the investigator would like to study microtubule disruption in cell cultures after various drug treatments. They are also interested in the co-localization of the microtubules with the Golgi and ER. Does anyone have experience with studying microtubules in plastic sections? What is the ideal fixation protocol to preserve the microtubules? Any advice on the subject is much appreciated!

Thank you!
-Nick

Nicholas Conoan
Electron Microscopy Specialist
Wittson Hall 2014
986395 Nebraska Medical Center
Omaha, NE 68198-6395
402-559-7292


The information in this e-mail may be privileged and confidential, intended only for the use of the addressee(s) above. Any unauthorized use or disclosure of this information is prohibited. If you have received this e-mail by mistake, please delete it and immediately contact the sender.


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From: vlad_speransky-at-tedpella.com
Date: Thu, 17 Aug 2017 13:04:04 -0500
Subject: [Microscopy] RE: Preservation of microtubules

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Nick,

Microtubules are not particularly tricky to preserve, just make sure the GA
fixative is *not* cold, as that promotes disassembly. There was a lot of
such work done in early 80s, I would rather not give just one name and thus
leave others out... So called PHEM buffer (originally from Jan de Mey, I
think?..) proved particularly good for cytoskeleton, for light and EM. It
does result in rather dense (meaning better preserved) cytoplasm, but that
should be less of an issue these days with the good digital cameras. Many
folks in those old days would rely on more extractive buffers to improve
visibility of the microtubules, sometimes even resorting to a mild osmotic
shock.

Feel welcome to respond to me directly for the names and more detail.

Best wishes,
Vlad

Vlad Speransky, PhD
Life Sciences Product Specialist
Ted Pella, Inc.
http://www.tedpella.com/
530-243-2200 ext. 266
Cell 530-768-3953
vlad_speransky-at-tedpella.com



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Hello All:

Our facility has received a project inquiry in which the investigator would
like to study microtubule disruption in cell cultures after various drug
treatments. They are also interested in the co-localization of the
microtubules with the Golgi and ER. Does anyone have experience with
studying microtubules in plastic sections? What is the ideal fixation
protocol to preserve the microtubules? Any advice on the subject is much
appreciated!

Thank you!
-Nick

Nicholas Conoan
Electron Microscopy Specialist
Wittson Hall 2014
986395 Nebraska Medical Center
Omaha, NE 68198-6395
402-559-7292


The information in this e-mail may be privileged and confidential, intended
only for the use of the addressee(s) above. Any unauthorized use or
disclosure of this information is prohibited. If you have received this
e-mail by mistake, please delete it and immediately contact the sender.


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From: Z.Zhou-at-lboro.ac.uk
Date: Fri, 18 Aug 2017 04:17:04 -0500
Subject: [Microscopy] TEM: softwares to aid selected area diffraction patterns indexing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Fellow Microscopists,
How do you index electron diffraction patterns recorded by TEM/SAED? Especially for those likely to be of hexagonal, orthorhombic or tetragonal etc. complex crystal structure? I'm looking for some software to aid pattern indexing, commercial or free as long as they are reliable and easy to use. Any suggestions would be highly appreciated please...
Zhou

Dr Z Zhou
Research Fellow
Loughborough Materials Characterisation Centre
Department of Materials
Loughborough University
Loughborough LE11 3TU
UK
01509 223163



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4, 79 -- Subject: TEM: softwares to aid selected area diffraction patterns indexing
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From: philippe.buffat-at-epfl.ch
Date: Fri, 18 Aug 2017 07:55:59 -0500
Subject: [Microscopy] TEM: softwares to aid selected area diffraction patterns indexing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dr Z Zhou asked: How do you index electron diffraction patterns recorded
by TEM/SAED?

You may consider JEMS from P. Stadelmann. Have a look to
http://www.jems-saas.ch/ that also contain the access to the limited
"Student version" for demo on Mac, PC and Linux.

This software is a comprehensive program for (HR) TEM+STEM image
simulation and electron diffraction (SAED, nano-diff, CBED, Kikuchi,
precession, powder patterns) interpretation and simulation based on
Bloch waves and multislice approaches.

In the present case, feeding JEMS with files containing the crystal
parameters for all suspected phases allows an automatic match with the
experimental patterns for phase identification.

Disclaimer: P. Stadelmann and myself were working in the same laboratory
for long though on different subjects.

P. Buffat


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From: microscopy.listserver-at-gmail.com
Date: Fri, 18 Aug 2017 19:59:24 -0500
Subject: [Microscopy] viaWWW:used TEM holder Talos

Contents Retrieved from Microscopy Listserver Archives
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X-from: ilchat.sabirov-at-imdea.org

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Email: ilchat.sabirov-at-imdea.org Name: Ilchat

Organization: IMDEA Materials Institute

Title-Subject: [Filtered] used TEM holder

Message: Dear Colleagues,

We would like to buy a used low background double tilt TEM holder compatible with TALOS. We were
searching with Google, but we could not find so far on sale such a holder.
Could you recommend any web-site or any company which can potentially sale such equipment? Thank you
in advance!

With best regards,
Ilchat

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From: microscopy.listserver-at-gmail.com
Date: Sat, 19 Aug 2017 06:05:43 -0500
Subject: [Microscopy] Fwd: How are grids made

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Email: brazda-at-fzu.cz Name: Petr Brazda

Organization: Institute of Physics of the Czech Academy of Science (Prague)

Title-Subject: [Filtered] Dewar for cold trap (CM 120)

Message: Hello,

We just broke our Dewar for the cold trap on our CM 120. Anyone has a spare one to sell?

Best regards,

Petr

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From jeverett397-at-gmail.com Fri Aug 18 21:31:42 2017
Return-Path: {jeverett397-at-gmail.com}
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X-from: Bargar, Tom W {tbargar-at-unmc.edu}

Dear Listers,

Does anyone out there know how the TEM grids we all use are made? I've
always been curious, but have not been able to find any information.

Tom Bargar
UNMC
Electron Microscopy Core Facility
986395 Nebraska Medical Center
Omaha, NE 68198-6395
402-559-7347
tbargar-at-unmc.edu


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From: microscopy.listserver-at-gmail.com
Date: Sat, 19 Aug 2017 06:07:05 -0500
Subject: [Microscopy] Fwd: Re: viaWWW:used TEM holder Talos

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X-from: joel paul {joel-at-vibeng.com}

Contact TSS Microscopy in Beaverton Oregon.

Very best regards,

Joel

Sent from my iPhone

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} Email: ilchat.sabirov-at-imdea.org Name: Ilchat
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} Title-Subject: [Filtered] used TEM holder
}
} Message: Dear Colleagues,
}
} We would like to buy a used low background double tilt TEM holder compatible with TALOS. We were
} searching with Google, but we could not find so far on sale such a holder.
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From: vray-at-partbeamsystech.com
Date: Sat, 19 Aug 2017 08:24:44 -0500
Subject: [Microscopy] Re: Fwd: How are grids made

Contents Retrieved from Microscopy Listserver Archives
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Hi Tom,

I wouldn't know how were made grids you buy from distributors as everyone keeps this "highly confidential," but I've had custom grids produced by process called "Photochemical Etching" or "Photochemical machining:"

https://en.wikipedia.org/wiki/Photochemical_machining

It is either one-sided process which can only cut the parts loose, or a two-sided process with exposure and etching happening from both sides of the metal sheet. With two-sided process recesses are easily produced by exposing one side only and etching 50% of the metal sheet thickness. There are plenty of OEMs in US, Europe, and China, who would happily produce custom grids to your design within the limitations of available to them process, if you could fork up one-time tooling charge (usually couple hundred dollars) and put up with minimal order requirement which varies between vendors from 1 or 2 sheet sized from 8/5"x11" to 1mx1m.

Stay curious :)
Valery

Valery Ray
==============================
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Methuen, MA 01844, USA
Phone: +1-978-305-0479 - leave a message
Mobie: +1-978-305-0479 - leave a message
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Web: www.freudlabs.com

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On Sat, 8/19/17, {microscopy.listserver-at-gmail.com} wrote:

Subject: [Microscopy] Fwd: How are grids made
To: vray-at-partbeamsystech.com
Date: Saturday, August 19, 2017, 7:07 AM




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X-from: Bargar, Tom W {tbargar-at-unmc.edu}

Dear Listers,

Does anyone out there know how the TEM
grids we all use are made?  I've
always been curious, but have not been
able to find any information.

Tom Bargar
UNMC
Electron Microscopy Core Facility
986395 Nebraska Medical Center
Omaha, NE 68198-6395
402-559-7347
tbargar-at-unmc.edu


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From: microscopy.listserver-at-gmail.com
Date: Sun, 20 Aug 2017 16:26:03 -0500
Subject: [Microscopy] Re: Fwd: How are grids made

Contents Retrieved from Microscopy Listserver Archives
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X-from: Bill & Sue Tivol {wtivol-at-sbcglobal.net}

Hi Tom,
I think some grids, including the standard copper ones, are made by
electrodeposition. I am pretty sure that different processes are used
just by looking at the edges of the grid bars. Molybdenum grids, for
example, have much rougher grid bars than copper or titanium grids.
Yours,
Bill




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From: benada-at-biomed.cas.cz
Date: Mon, 21 Aug 2017 06:56:25 -0500
Subject: [Microscopy] Re: viaWWW:Dewar for cold trap (CM 120)

Contents Retrieved from Microscopy Listserver Archives
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Hello,
I've just replied to Petr in Czech off-line.

Here is a summary:
The producer of Dewar for cold trap used in Philips CM microscopes is also offering Glass Refills:
{http://www.kgw-isotherm.com/produkte/dewar/00.html}

Best regards

Oldrich

--
Oldřich Benada
Institute of Microbiology CAS, v.v.i.
Laboratory of Molecular Structure Characterization
Vídeňská 1083
142 20 Prague 4
Czech Republic

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From: microscopy.listserver-at-gmail.com
Date: Mon, 21 Aug 2017 10:26:59 -0500
Subject: [Microscopy] viaWWW:EELS plasmon peak analysis

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Email: f.long-at-queensu.ca Name: Fei Long

Organization: Queen's University

Title-Subject: [Filtered] EELS plasmon peak analysis

Message: Hi there,

I am looking for a good method in fitting the EELS plasmon peak for
identification phases with subtle hydrogen concentration differences.
The NLLS gaussian fitting function in the Gatan GMS software seems not
able to detect the very small energy shift. If any EELS expert can give
suggestions in analyzing the plasmon peak that will be great.

Best regards,

Fei


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From: steven.spurgeon-at-pnnl.gov
Date: Mon, 21 Aug 2017 13:11:03 -0500
Subject: [Microscopy] Re: viaWWW:EELS plasmon peak analysis

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Hi Fei,

Hyperspy has an excellent library of EELS functions that can help you do this in an automated fashion. It’s written in Python, so the syntax isn’t too difficult to learn.

Here’s a link: http://hyperspy.org/hyperspy-doc/current/user_guide/eels.html

______________________________________
Steven R. Spurgeon, Ph.D.
Postdoctoral Research Associate
Physical and Computational Sciences Directorate

Pacific Northwest National Laboratory
902 Battelle Boulevard
P.O. Box 999 MSIN:K8-87
Richland, WA 99352

Tel: +1-509-371-7123
steven.spurgeon-at-pnnl.gov
www.stevenspurgeon.com {http://www.stevenspurgeon.com/}
www.pnnl.gov {http://www.pnnl.gov/}


On 8/21/17, 8:49 AM, "microscopy.listserver-at-gmail.com" {microscopy.listserver-at-gmail.com} wrote:




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Email: f.long-at-queensu.ca Name: Fei Long

Organization: Queen's University

Title-Subject: [Filtered] EELS plasmon peak analysis

Message: Hi there,

I am looking for a good method in fitting the EELS plasmon peak for
identification phases with subtle hydrogen concentration differences.
The NLLS gaussian fitting function in the Gatan GMS software seems not
able to detect the very small energy shift. If any EELS expert can give
suggestions in analyzing the plasmon peak that will be great.

Best regards,

Fei


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From: eikonika-at-otenet.gr
Date: Tue, 22 Aug 2017 05:52:43 -0500
Subject: [Microscopy] JSM5600LV evacuation arrest

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Hello
Our Jeol SEM JSM5600LV doesn't get vacuum ready. Normally pre-evacuation and
evacuation phases last 2' each, now pre-evacuation lasts 2' but evacuation
phase seems endless After 1h no vacuum ready appeared neither any message
from the software.
Can be mechanical or electronic failure or both?
Any help to tackle the p[problem will be greatly appreciated
yorgos


Dr Yorgos Nikas
Athens Innovative Microscopy
Skra 36 Voula 16673 GREECE

Tel/fax +30 210 8957677
mobile +30 6945 107477
www.eikonika.netwww.aim.cat
*************************************




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From: benada-at-biomed.cas.cz
Date: Tue, 22 Aug 2017 06:30:02 -0500
Subject: [Microscopy] Re: JSM5600LV evacuation arrest

Contents Retrieved from Microscopy Listserver Archives
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Hello Yorgos,

Personally I do not have any experiences with SEM JSM5600LV. However, a long time ago we had a similar problem on Balzer BAF 301 Freeze etching device. The problem was in a pirani gauge. It was heavily contaminated. Therefore the vacuum level readout was completely wrong.
In the Balzers manual there was a procedure how to clean the pirani gauge and it was working well. The cleaning of the pirani gauge solved our problem. So, I would suggest to check pirani gauge in your SEM. It might help you.

Best regards

Oldrich

--
Oldřich Benada
Institute of Microbiology CAS, v.v.i.
Laboratory of Molecular Structure Characterization
Vídeňská 1083
142 20 Prague 4
Czech Republic

On Tue, 22 Aug 2017 05:56:25 -0500, eikonika-at-otenet.gr wrote :
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} Hello
} Our Jeol SEM JSM5600LV doesn't get vacuum ready. Normally
} pre-evacuation and evacuation phases last 2' each, now pre-evacuation
} lasts 2' but evacuation phase seems endless After 1h no vacuum ready
} appeared neither any message from the software.
} Can be mechanical or electronic failure or both?
} Any help to tackle the p[problem will be greatly appreciated
} yorgos
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From: hsia627-at-hotmail.com
Date: Tue, 22 Aug 2017 10:57:15 -0500
Subject: [Microscopy] Preservation of myelin ultrastructure in TEM embedding

Contents Retrieved from Microscopy Listserver Archives
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Hi, everyone,

Our facility provides EM support to several research projects studying ultrastructural changes of the brain, optic nerve, and other neural tissues using large animals such as dogs and monkeys. We often see various degrees of myelin sheet bubbling and occasional interstitial edema of mitochondria even in control (untreated) samples. The distortions are often inconsistent and the degree can vary from one specimen block to another. We suspect it is a fixation problem as it is difficult to do perfusion fix with large animals and sometimes impossible as the researchers often need to collect samples for biochemical analysis as well. I am just wondering if anyone has some insights or a workaround to minimize the myelin distortion. I would also appreciate any tips on preserving myelin ultrastructure during TEM embedding.
Thanks.

Best regards,
Ru-ching Hsia
Electron Microscopy Core Imaging Facility
University of Maryland Baltimore



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From: microscopy.listserver-at-gmail.com
Date: Wed, 23 Aug 2017 05:34:31 -0500
Subject: [Microscopy] viaWWW: EELS & EFTEM Analysis Training School - October 2017

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi, Sorry for not providing details in my original message. There are several research groups working on various projects with us. The best myelin preservation we have gotten so far is when the researcher used 4% PFA in phosphate buffer for perfusion. As soon as the sample is delivered to us (perhaps one to two hours later), we tried to trim down the specimen (~5mm x2mm) in PFA and transfer to 2% PFA/2.5% glutaraldehyde fixative and leave it overnight before processing. We have been using 1% osmium for 30 min and then reduced osmium for 30 min both on ice and 1% UA in water at room temp, gradually dehydrate up to 100% ETOH, then 100% acetone. and embed in AralditeEopon resin. We still see mild bubbling in 25 to 50% of myelin under this condition. I am wondering whether it would be better if we asked the researchers to transfer the specimen directly in a fixative containing glutaraldehyde immediately after dissecting out the tissue. Surgeries involving large animals are often very involved and costly. The researchers often need to collect a large number of tissue samples for multiple tests in limited time. We also do not have the luxuaries to perform trial experiments to test and optimize the best collecting and processing workflow.
I hope some members of the list may have similar experience and can recommend best practice for fixation and sample collection under these constrained conditions.

Thank you for your input.


Ru-ching Hsia

________________________________________
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Email: jhyun-at-gatan.com Name: John Hyun

Organization: Gatan

Title-Subject: [Filtered] EELS & EFTEM Analysis Training School - October 2017

Message: October 17-20, 2017
Pleasanton, CA, US

Electron energy loss spectroscopy (EELS) is a powerful technique that provides both compositional
and chemical information from sub-nanometer areas in the sample. As a course attendee, you will
learn best practices to set up and optimize your EELS hardware and experimental protocols so you can
capture and extract the maximum amount of compositional and chemical information from your TEM
samples. Topics include:

-Fundamentals of EELS and energy-filtered imaging in TEM
-Principles of operation of EFTEM and EELS systems
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-Quantification of elemental composition
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-Use of EELS signals to form maps of elemental and chemical composition
-EFTEM and STEM EELS spectrum imaging techniques
-Identification of material phases via EELS fine structure mapping
-Applications to biological and physical science specimens

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From: eikonika-at-otenet.gr
Date: Wed, 23 Aug 2017 07:02:37 -0500
Subject: [Microscopy] RE: JSM5600LV evacuation arrest

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi again

Thanks for your responses. As you suggested, I checked the diffusion pump
heater and the pirani gauge, both looked fine. It turned out that the
problem was a tiny bundle of dust fibers situated at the o ring sealing the
specimen chamber. After some cleaning the scope goes vacuum ready but needs
considerably more time, so I guess more cleaning may help further.
Best regards
yorgos


Hello
Our Jeol SEM JSM5600LV doesn't get vacuum ready. Normally pre-evacuation and
evacuation phases last 2' each, now pre-evacuation lasts 2' but evacuation
phase seems endless After 1h no vacuum ready appeared neither any message
from the software.
Can be mechanical or electronic failure or both?
Any help to tackle the p[problem will be greatly appreciated yorgos


Dr Yorgos Nikas
Athens Innovative Microscopy
Skra 36 Voula 16673 GREECE

Tel/fax +30 210 8957677
mobile +30 6945 107477
www.eikonika.net www.aim.cat
*************************************



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From: microscopy.listserver-at-gmail.com
Date: Wed, 23 Aug 2017 07:31:10 -0500
Subject: [Microscopy] TEM: softwares to aid selected area diffraction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Scott Walck {S.Walck-at-comcast.net}

In addition to JEMS, which Philippe Buffat described, there are also CSpot
and Electron Diffraction. CSpot is commercial software that can help you
index spot patterns, Kikuchi patterns, and ring patterns. There is a
downloadable demo version. Electron Diffraction was written by Jean Paul
Morniroli and can be downloaded for free. It does have problems with
Rhombohedral crystal structure and he recommended that I use JEMS for B4C.

-Scott

-----Original Message-----
X-from: Z.Zhou-at-lboro.ac.uk [mailto:Z.Zhou-at-lboro.ac.uk] Sent: Friday, August 18, 2017 5:34 AM
To: S.Walck-at-comcast.net

Dear Fellow Microscopists,
How do you index electron diffraction patterns recorded by TEM/SAED?
Especially for those likely to be of hexagonal, orthorhombic or tetragonal
etc. complex crystal structure? I'm looking for some software to aid pattern
indexing, commercial or free as long as they are reliable and easy to use.
Any suggestions would be highly appreciated please...
Zhou

Dr Z Zhou
Research Fellow
Loughborough Materials Characterisation Centre Department of Materials
Loughborough University Loughborough LE11 3TU UK
01509 223163


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From: xin-at-magnet.fsu.edu
Date: Wed, 23 Aug 2017 09:36:22 -0500
Subject: [Microscopy] TEM: softwares to aid selected area diffraction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

There is another available software that TEM users might be interested.

Landyne software suit can used for simulation and index for spot and ring
SAED patterns (http://www.unl.edu\ncmn-cfem/xzli\computer-programs).


Yan Xin
NHMFL/FSU

-----Original Message-----
X-from: philippe.buffat-at-epfl.ch [mailto:philippe.buffat-at-epfl.ch]
Sent: Friday, August 18, 2017 8:57 AM
To: xin-at-magnet.fsu.edu

Dr Z Zhou asked: How do you index electron diffraction patterns recorded
by TEM/SAED?

You may consider JEMS from P. Stadelmann. Have a look to
http://www.jems-saas.ch/ that also contain the access to the limited
"Student version" for demo on Mac, PC and Linux.

This software is a comprehensive program for (HR) TEM+STEM image
simulation and electron diffraction (SAED, nano-diff, CBED, Kikuchi,
precession, powder patterns) interpretation and simulation based on Bloch
waves and multislice approaches.

In the present case, feeding JEMS with files containing the crystal
parameters for all suspected phases allows an automatic match with the
experimental patterns for phase identification.

Disclaimer: P. Stadelmann and myself were working in the same laboratory
for long though on different subjects.

P. Buffat


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From: xin-at-magnet.fsu.edu
Date: Wed, 23 Aug 2017 09:42:53 -0500
Subject: [Microscopy] TEM: softwares to aid selected area diffraction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

There is another available software that TEM users might be interested.

Landyne software suit can used for simulation and index for spot and ring
SAED patterns (http://www.unl.edu\ncmn-cfem/xzli\computer-programs).


Yan Xin
NHMFL/FSU

-----Original Message-----
X-from: philippe.buffat-at-epfl.ch [mailto:philippe.buffat-at-epfl.ch]
Sent: Friday, August 18, 2017 8:57 AM
To: xin-at-magnet.fsu.edu

Dr Z Zhou asked: How do you index electron diffraction patterns recorded
by TEM/SAED?

You may consider JEMS from P. Stadelmann. Have a look to
http://www.jems-saas.ch/ that also contain the access to the limited
"Student version" for demo on Mac, PC and Linux.

This software is a comprehensive program for (HR) TEM+STEM image
simulation and electron diffraction (SAED, nano-diff, CBED, Kikuchi,
precession, powder patterns) interpretation and simulation based on Bloch
waves and multislice approaches.

In the present case, feeding JEMS with files containing the crystal
parameters for all suspected phases allows an automatic match with the
experimental patterns for phase identification.

Disclaimer: P. Stadelmann and myself were working in the same laboratory
for long though on different subjects.

P. Buffat


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16, 116 -- Subject: RE: TEM: softwares to aid selected area diffraction patterns indexing
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From: microscopy.listserver-at-gmail.com
Date: Thu, 24 Aug 2017 18:21:55 -0500
Subject: [Microscopy] viaWWW:Open position: Research Associate I (28N) - Electron

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Email: nikd-at-vsl.cua.edu Name: Nik Deems

Organization: VSL

Title-Subject: [Filtered] Backscatter detector

Message: Might anyone have a lead on a used BSE detector for a JEOL-5910?

Cheers,
Nik Deems
Electron Microscopy Lab Technician
Vitreous State Laboratory

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10, 52 -- Subject: viaWWW:Backscatter detector
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From kelnanc57-at-gmail.com Thu Aug 24 17:44:29 2017
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Email: cni-at-udel.edu Name: Chaoying Ni

Organization: University of Delaware

Title-Subject: [Filtered] Open position: Research Associate I (28N) - Electron Microscopy

Message: Research Associate I - (Grade 28N) Materials Science and Engineering

Deadline: September 7, 2017

CONTEXT OF THE JOB:

Under limited supervision, performs a variety of complex technical laboratory duties including the
design and construction of test equipment. Supervises and trains personnel. The principal emphasis
is on working independently and exercising personal initiative in conducting experiments using
complex electron and light microscopes, scanning probe microscopes and associated sample preparation
equipment and experiment devices.

QUALIFICATIONS:

Requires a minimum of a Bachelor's degree and one year of experience in laboratory work in a
relevant field as well as experience with the necessary physical, electronic and chemical systems.
Supervisory experience preferred.

Related progressive experience beyond a high school diploma or GED may be substituted for required
education or additional related education may be substituted for required experience.
Requires knowledge of laboratory techniques, procedures and instrumentation.

Requires abilities to read and interpret complex operations manuals, drawings and design, interpret
problems and design equipment for the particular tasks, assume responsibility and take initiative to
perform tasks, work independently, train and instruct others, and communicate effectively and
interact well with people of all ages and diverse backgrounds.

OCCUPATIONAL EXPOSURES:

May be required to use personal protective equipment to prevent exposure to hazardous materials.


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From: Z.Zhou-at-lboro.ac.uk
Date: Wed, 30 Aug 2017 09:31:22 -0500
Subject: [Microscopy] HRTEM sample prep for cross section imaging of plate-like

Contents Retrieved from Microscopy Listserver Archives
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Hello,

I recently got a second hand Noran Quest EDX system with a SiLi detector
and was wondering if anyone on this list has technical information to share.

The system is currently cooling down with its Dewar filled with LN2.
In the "Quest Status" application I get various voltage measurements
including a voltage for the LN sensor (currently at 4.33V and slowly
decreasing) and a voltage for the detector temperature (currently at
0V). The application also displays a warning "LN error - Bias off".

While I believe that the system has not cooled down sufficiently yet
(and hence the bias cannot be turned on without causing damage to the
detector), I'm a bit puzzled that the detector temperature voltage is
constantly at 0V. I would rather expect a voltage } 0V here that reflects
on the current temperature of the detector.

In addition, it would be great to get an idea how those measurement
voltages can be transformed into actual temperatures.


Thank you,
Stefan

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From donahenr845uz-at-gmail.com Fri Aug 25 08:15:17 2017
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Dear Colleagues,
Plate-like nanoparticles (2-5micron diameter, 20-100nm thick) have been prepared. Plan view was examined by the usual powder dispersion drop cast on a holey-C film method. But, we now would like to look at the cross sections of these particles to study how the atomic layers stack and grow into a plate. The sample comes in water suspension.
Two questions:
1, sample prep for cross sections HREM. How do we prepare TEM specimens for high-reso atomic imaging of the cross sections of these thin nano-plates?
2, Good statistics. How can we maximise the number (~30 or more) of the nano-plates in one specimen?
Many thanks for your help.
Zhou
Dr Z Zhou, Loughborough University, UK


==============================Original Headers==============================
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From: j.sharp-at-sheffield.ac.uk
Date: Wed, 30 Aug 2017 10:26:17 -0500
Subject: [Microscopy] Re: HRTEM sample prep for cross section imaging of plate-like

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

What are these plates like - brittle or ductile? Electrically
conductive? Magnetic? Hard? What are they like to ion mill?

My instinct says embed them in epoxy, squash it as it sets so they
want to line up perpendicular to the compression, then turn the glued
block round 90 degrees, take a section, and mechanically thin and ion
mill it. But if they are resistant to ion milling the block might just
fall apart as the epoxy mills away instead, or you will get thin epoxy
and thick useless bits of plates. If you encapsulate them in some soft
metal and do a similar thing they won't be aligned so well and if they
are brittle they will break up and end up mostly the wrong way.

Looking at the size, you could *almost* pick them up one by one with a
micromanipulator, stick them to something parallel, and FIB the whole
stack, given that you are meticulous and persistent. But this may be
just a fancy way of having a terrible week at work.

Jo

On 30 August 2017 at 15:47, {Z.Zhou-at-lboro.ac.uk} wrote:
}
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} Dear Colleagues,
} Plate-like nanoparticles (2-5micron diameter, 20-100nm thick) have been prepared. Plan view was examined by the usual powder dispersion drop cast on a holey-C film method. But, we now would like to look at the cross sections of these particles to study how the atomic layers stack and grow into a plate. The sample comes in water suspension.
} Two questions:
} 1, sample prep for cross sections HREM. How do we prepare TEM specimens for high-reso atomic imaging of the cross sections of these thin nano-plates?
} 2, Good statistics. How can we maximise the number (~30 or more) of the nano-plates in one specimen?
} Many thanks for your help.
} Zhou
} Dr Z Zhou, Loughborough University, UK
}
}
} ==============================Original Headers==============================
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} 2, 80 -- From: Zhaoxia Zhou {Z.Zhou-at-lboro.ac.uk}
} 2, 80 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com}
} 2, 80 -- Subject: HRTEM sample prep for cross section imaging of plate-like
} 2, 80 -- nanoparticles
} 2, 80 -- Thread-Topic: HRTEM sample prep for cross section imaging of plate-like
} 2, 80 -- nanoparticles
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From: steven.spurgeon-at-pnnl.gov
Date: Wed, 30 Aug 2017 16:41:13 -0500
Subject: [Microscopy] Sound proofing in TEM lab

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Zhou,

Jo mentioned something similar to this already, but Schreiber et al.
have a nice paper showing correlated plan view and x-section imaging
of nanostructures via FIB preparation. The reference is here:

https://www.cambridge.org/core/journals/microscopy-and-microanalysis/article/div-classtitlea-method-for-directly-correlating-site-specific-cross-sectional-and-plan-view-transmission-electron-microscopy-of-individual-nanostructuresdiv/943F93922F6E080BECE62285FF81BEAB

This probably won't satisfy your requirement of having tens of
platelets in a single cross section sample, but I suppose you could
deposit a high concentration on a support film and hunt around until
you find a area with many stacked like shingles. Or as Jo mentioned,
this all may be a great way to punish a graduate student!

Good luck,
Chris

On Wed, Aug 30, 2017 at 10:43 AM, {Z.Zhou-at-lboro.ac.uk} wrote:
}
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} Dear Colleagues,
} Plate-like nanoparticles (2-5micron diameter, 20-100nm thick) have been prepared. Plan view was examined by the usual powder dispersion drop cast on a holey-C film method. But, we now would like to look at the cross sections of these particles to study how the atomic layers stack and grow into a plate. The sample comes in water suspension.
} Two questions:
} 1, sample prep for cross sections HREM. How do we prepare TEM specimens for high-reso atomic imaging of the cross sections of these thin nano-plates?
} 2, Good statistics. How can we maximise the number (~30 or more) of the nano-plates in one specimen?
} Many thanks for your help.
} Zhou
} Dr Z Zhou, Loughborough University, UK
}
}
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From annepenn4-at-gmail.com Wed Aug 30 13:37:01 2017
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Message-ID: {5B87926A.F755785F-at-gmail.com}

Hello everyone,

We’re in the process of installing a new aberration-corrected STEM and we’re trying to diagnose some noise in the 100-400 Hz range. In particular, our room has a rather high ceiling and we’re concerned about issues due to the room volume.

I’ve seen that some other laboratories use sound-dampening drapes and I’m wondering if these are common for rooms with high ceilings. Fire code issues make it difficult for us to set up these drapes, so it would help to know what experiences others have had.

Does anyone have any suggestions? Thanks!

______________________________________
Steven R. Spurgeon, Ph.D.
Postdoctoral Research Associate
Physical and Computational Sciences Directorate

Pacific Northwest National Laboratory
902 Battelle Boulevard
P.O. Box 999 MSIN:K8-87
Richland, WA 99352

Tel: +1-509-371-7123
steven.spurgeon-at-pnnl.gov
www.stevenspurgeon.com {http://www.stevenspurgeon.com/}
www.pnnl.gov {http://www.pnnl.gov/}




==============================Original Headers==============================
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8, 26 -- Subject: Sound proofing in TEM lab
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From: matthew.weyland-at-monash.edu
Date: Wed, 30 Aug 2017 17:15:55 -0500
Subject: [Microscopy] Re: HRTEM sample prep for cross section imaging of plate-like

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Zhou,

We faced a similar problem trying to look at the cross sectional
structure of hard nanowire heterostructures (See Jiang et al
Nanoletters, 13, 5135 2013 and Zheng et al. nanoletters 13, 3742 2013)
In the end we borrowed a page from our biological colleagues and
embedded and microtomed them! Surprisingly successful, you will have to
do this very carefully at liq nitrogen temperatures with a diamond
knife. If you want the details of microtoming hard materials look up the
papers by Selwyn Glanville. The biggest struggle you will have is
getting your plates to line up and disperse for the embedding (easy with
epitaxially grown samples). This will also give you MANY plates in
x-section, so plenty of statistics.

Regards

Matthew

} Dear Colleagues,
} Plate-like nanoparticles (2-5micron diameter, 20-100nm thick) have
} been prepared. Plan view was examined by the usual powder dispersion
} drop cast on a holey-C film method. But, we now would like to look at
} the cross sections of these particles to study how the atomic layers
} stack and grow into a plate. The sample comes in water suspension.
} Two questions:
} 1, sample prep for cross sections HREM. How do we prepare TEM
} specimens for high-reso atomic imaging of the cross sections of these
} thin nano-plates?
} 2, Good statistics. How can we maximise the number (~30 or more) of
} the nano-plates in one specimen?
} Many thanks for your help.
} Zhou
} Dr Z Zhou, Loughborough University, UK

--


*Dr Matthew Weyland *
Associate Professor

*Monash Centre for Electron Microscopy and Department of Materials
Science and Engineering*
10 Innovation Walk, Clayton Campus
Monash University
Clayton VIC 3800 Australia

T: +61 3 9905 9026
E: matthew.weyland-at-monash.edu {mailto:name.surname-at-monash.edu}
mcem.monash.edu {http://mcem.monash.edu/}

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From: steven.spurgeon-at-pnnl.gov
Date: Thu, 31 Aug 2017 10:31:38 -0500
Subject: [Microscopy] Re: Sound proofing in TEM lab

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi everyone,

Thank you all for your responses.

I thought it would be helpful to summarize the emails I received. The responses were mixed, but most people said that curtains will not help in the 100-400 Hz frequency range.

There were a few suggestions that the source might be the air diffusers or somewhere else in the ventilation system, so we will look into this in more detail. As far as solutions, some people suggested acoustic traps and various insulation options, but it seems that this frequency range is difficult to block out.

We’ll keep hunting!

______________________________________
Steven R. Spurgeon, Ph.D.
Postdoctoral Research Associate
Physical and Computational Sciences Directorate

Pacific Northwest National Laboratory
902 Battelle Boulevard
P.O. Box 999 MSIN:K8-87
Richland, WA 99352

Tel: +1-509-371-7123
steven.spurgeon-at-pnnl.gov
www.stevenspurgeon.com {http://www.stevenspurgeon.com/}
www.pnnl.gov {http://www.pnnl.gov/}


On 8/30/17, 3:45 PM, "Spurgeon, Steven R" {steven.spurgeon-at-pnnl.gov} wrote:

Hello everyone,

We’re in the process of installing a new aberration-corrected STEM and we’re trying to diagnose some noise in the 100-400 Hz range. In particular, our room has a rather high ceiling and we’re concerned about issues due to the room volume.

I’ve seen that some other laboratories use sound-dampening drapes and I’m wondering if these are common for rooms with high ceilings. Fire code issues make it difficult for us to set up these drapes, so it would help to know what experiences others have had.

Does anyone have any suggestions? Thanks!

______________________________________
Steven R. Spurgeon, Ph.D.
Postdoctoral Research Associate
Physical and Computational Sciences Directorate

Pacific Northwest National Laboratory
902 Battelle Boulevard
P.O. Box 999 MSIN:K8-87
Richland, WA 99352

Tel: +1-509-371-7123
steven.spurgeon-at-pnnl.gov
www.stevenspurgeon.com {http://www.stevenspurgeon.com/}
www.pnnl.gov {http://www.pnnl.gov/}






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10, 28 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
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From: adam.schrofel-at-gmail.com
Date: Thu, 31 Aug 2017 11:01:03 -0500
Subject: [Microscopy] Biology TEM camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hallo everybody,

we are going to buy a routine 120 kV TEM, mainly as a plastic sections, negative staining etc. workhorse. Nevertheless, one part of the tender will be also a cryoholder for the very preliminary screening of the SPA grids and a little bit of CEMOVIS. As I relatively understand to most of the microscope parameters (there are more or less 3 potential guys - Jeol 1400+, FEI Talos 120 kV and Hitachi HT7800), I really don’t know how to choose the camera. It should be bottom-mounted and more sensitive than the basic cameras. I am fully aware that we cannot afford a direct detector by far (we don’t even reach the other CCD cameras from Gatan…), but - could somebody tell me what features and parameters I should follow, which numbers are describing what? There is actually almost no materials, leaflets and brochures about the camera of the above-mentioned producers (or their third-party suppliers). Please advise, even the tips for the actual camera type.

Best regards

Adam Schrofel
Faculty of Nature, Charles University, Prague

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From: microscopy.listserver-at-gmail.com
Date: Fri, 1 Sep 2017 05:16:54 -0500
Subject: [Microscopy] viaWWW: Desktop Carbon Coater

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Email: wagneran-at-uwec.edu Name: Tony Wagner

Organization: University of Wisconsin - Eau Claire

Title-Subject: [Filtered] Desktop Carbon Coater

Message: Hello Everyone

We are looking to upgrade our carbon coater from an old Edwards Evaporator using rods to a carbon
filament in a desktop unit. I have no experience with the carbon fiber systems and am looking for
feedback from anyone who has used a carbon fiber based carbon coater in a desktop unit. I am
currently looking at the the Denton Desk V HP with tilt and rotation. Have you been happy with the
conductive coatings produced and if not what have the issues been.

Thank you in advance for you response.


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From: microscopy.listserver-at-gmail.com
Date: Sat, 2 Sep 2017 06:51:49 -0500
Subject: [Microscopy] viaWWW:Job opening: Research Analyst 3 (TEM)

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Email: zhiqiang-at-pdx.edu Name: Zhiqiang Chen

Organization: Center for Electron Microscopy and Nanofabrication

Title-Subject: [Filtered] Job opening: Research Analyst 3 (TEM)

Message: Center for Electron Microscopy and Nanofabrication at Portland State University has an open
position of Research Analyst 3 (TEM). Online application is available at
https://jobs.hrc.pdx.edu/postings/24142. Thank you for your attention.

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From: microscopy.listserver-at-gmail.com
Date: Sun, 3 Sep 2017 06:34:36 -0500
Subject: [Microscopy] viaWWW:Postdoc Position in Functional Nanoparticles and Emulsion

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Email: tamarps-at-technion.ac.il Name: Tamar Segal-Peretz

Organization: Technion- Israel Institute of Technology

Title-Subject: [Filtered] Postdoctoral position
Message: Postdoc Position in Functional Nanoparticles and Emulsion Imaging

A postdoctoral position is available in a fascinating project on understanding the formation,
dynamics, and properties of new-type emulsions and bijels. The researcher will use the
state-of-the-art cryo-SEM and cryo-TEM facilities in the Electron Microscopy Center for Soft Matter
in the Department of Chemical Engineering, Technion, to study the relations between functionalized
nanoparticles and emulsion and bijels structure and behavior. The postdoc will be part of a
collaboration between the Technion-Israel Institute of Technology (Asst. Prof. Tamar Segal-Peretz’s
group and Asst. Prof. Ofer Manor’s group) and the University of Calgary, Canada (Asst. Prof. Milana
Trifkovic’s group and Prof. Steven L. Bryant’s group). The postdoc will work mainly at the Technion,
Haifa, Israel, with travel opportunities to Calgary.

Applicants should have a strong background in electron microscopy, preferably with cryo-imaging
experience. Additional background in colloidal science is an advantage. Ability to work in a
team, as well as excellent verbal and written communication skills are expected.

To apply, please send a cover letter, curriculum vitae, and 1-2 relevant published papers to the
e-mail below. The title of the email should read: Postdoctoral positionץ

Contact:
Tamar Segal-Peretz
Department of Chemical Engineering
Technion - Israel Institute of Technology
Email: tamarps-at-technion.ac.il
Website: http://fnai.technion.ac.il/


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From: ben.micklem-at-pharm.ox.ac.uk
Date: Tue, 5 Sep 2017 04:32:28 -0500
Subject: [Microscopy] vacuum for bio TEM non-cryo 120kV

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Dear All,

I need to compensate magnetic stray field influences on high working
distances in a field emission SEM. On my old SEM I had three pairs of
Helmholtz coils but now I intend to go to three single X / Y / Z
room-installed coils.

Without compensation the actual stray fields in a distance of ca. one
meter are ca. 20 nano-Tesla, near the specimen position on the outside
chamber wall ca. 30 nano-Tesla, with Z being a little higher than X and Y.

What I read is that room-installed coils will produce a more uniform
compensation field and Helmholtz coils might deal better with stronger
stray field influence.

I am grateful for any hints before deciding...


Best,

Stefan


--
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Stefan Diller - Scientific Photography
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D - 97072 Wuerzburg Germany
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Websites:
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Anfahrt: http://Mail.map24.com/Stefan.Diller
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From smithejoseph18k-at-gmail.com Mon Sep 4 22:47:20 2017
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Dear List,

We're buying a new TEM, and it is hard to come to a conclusion as to what sort of vacuum pump combination would be best for us.

We do 120kV biological TEM of resin-impregnated sections, no cryo work. Our specimens are beam stable, and we've not been using a cold finger anti-contamination design on our previous TEMs going back 30 years; we plan to continue without one. We vent the specimen airlock with air from the room- should we move to nitrogen? We will exclusively use LaB6 filaments.

If you could choose an ideal vacuum system combination to achieve the following ordered priorities, what pumps would it consist of?

1) Reliability- low maintenance, long lifetime
2) Speed- fast pump down from specimen changes, and from filament changes.
3) Clean- probably more for the cooled (-20 C) camera than anything else, but also to extend filament life and reduce contamination of specimen
4) Good ultimate vacuum- for increased lifetime of LaB6 filament
5) Quiet

My main questions are:
Is an Ion Getter pump needed, if we aren't doing cryo, so there will be comparatively little water vapour pumping?
Is there much difference in our application for turbo molecular pumps that are oil-lubricated vs magnetic levitation bearings?
Should we really be using a cold finger?
Should we be venting the specimen airlock with dry nitrogen?

Possible system 1:
Rotary pump, 2x oil diffusion pumps

Possible system 2:
Scroll pump, turbo molecular pump

Possible system 3:
Diaphragm pump, turbo molecular pump, large IGP on column, small IGP on gun.

Best regards,

Ben

--
Research Support Manager (Imaging and IT Systems)
MRC Brain Network Dynamics Unit,
University of Oxford Department of Pharmacology,
Mansfield Road, Oxford, OX1 3TH, United Kingdom.
Telephone: 01865 271897 {http://www.mrcbndu.ox.ac.uk/}


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From: kleopullin-at-email.arizona.edu
Date: Tue, 5 Sep 2017 10:35:45 -0500
Subject: [Microscopy] High-resolution and correlative microscopy for geologists

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Dear Microscopy Colleagues,


I've worked with Zeiss Raw Materials to organize the first general
short course on microscopy and spectroscopy for the 2017 Geological
Society of America Annual Meeting in Seattle. Short courses run right
before the meeting and are offered to both attendees and the public.


I'd love for you to join us there to look at imaging and
characterizing rocks with photons, electrons, ions, and x-rays.


High Resolution and Correlative Microscopy and Spectroscopy for the Geosciences


Saturday, October 21, 2017, from 8 AM to 5 PM


Washington State Convention Center, Meeting Room 212


The cost is $100 if you sign up by September 18th and you're
registered for the meeting, plus $40 for non-registrants and $30 after
early registration. The course is worth 0.8 CEUs, and lunch and coffee
breaks are provided.


Instructors: K. Leo Pullin, Consultant; Matthew Andrew, Carl Zeiss
X-Ray Microscopy; Sreenivas Bhattiprolu, Carl Zeiss X-Ray Microscopy.


http://community.geosociety.org/gsa2017/science-careers/courses


Please contact me via email if you have any questions.


Thanks, Kleo Pullin


------------------------------------------------------------------------------------------------------------------

K. Leo Pullin
San Pablo, CA
209-610-0555
kleopullin-at-email.arizona.edu
https://www.linkedin.com/in/kleopullin
https://twitter.com/resolvingdust

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From: microscopy.listserver-at-gmail.com
Date: Tue, 5 Sep 2017 15:35:21 -0500
Subject: [Microscopy] viaWWW:air table for ultramicrotome

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X-from: Jen.Fendler-at-utas.edu.au

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Email: Jen.Fendler-at-utas.edu.au Name: Jen Fendler

Organization: University of Tasmania

Title-Subject: [Filtered] air table for ultramicrotome

Message: Dear TEM experts

Are there any cons to purchasing an air table rather than a passive
antivibration table for an ultramicrotome?

Our granite table is inadequate most days (although it was built pseudo
in-house rather than commercial purpose built for ultramicrotome, and
was sufficient before major hospital renovations nearby), and our
situation is that our TEM facility is on the 5th floor, and we have
numerous major ongoing building works on the blocks adjacent to our
building, so I doubt even a move to the basement would help for the next
1-2 years.

I wondered why air tables are not commonly used for ultramicrotomes and
if there's any rationale against this, and would like to know before
purchasing one. I'm trialing one at the moment and it works well (other
than minor annoyance of how easily it is bumped by the slightest brush
of eyes on oculars etc)

Kind regards
Jen

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From: wtivol-at-sbcglobal.net
Date: Tue, 5 Sep 2017 18:09:47 -0500
Subject: [Microscopy] Re: vacuum for bio TEM non-cryo 120kV

Contents Retrieved from Microscopy Listserver Archives
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} On Sep 5, 2017, at 2:49 AM, ben.micklem-at-pharm.ox.ac.uk wrote:
}
}
} Dear List,
}
} We're buying a new TEM, and it is hard to come to a conclusion as to what sort of vacuum pump combination would be best for us.
}
} We do 120kV biological TEM of resin-impregnated sections, no cryo work. Our specimens are beam stable, and we've not been using a cold finger anti-contamination design on our previous TEMs going back 30 years; we plan to continue without one. We vent the specimen airlock with air from the room- should we move to nitrogen? We will exclusively use LaB6 filaments.
}
} If you could choose an ideal vacuum system combination to achieve the following ordered priorities, what pumps would it consist of?
}
} 1) Reliability- low maintenance, long lifetime
} 2) Speed- fast pump down from specimen changes, and from filament changes.
} 3) Clean- probably more for the cooled (-20 C) camera than anything else, but also to extend filament life and reduce contamination of specimen
} 4) Good ultimate vacuum- for increased lifetime of LaB6 filament
} 5) Quiet
}
} My main questions are:
} Is an Ion Getter pump needed, if we aren't doing cryo, so there will be comparatively little water vapour pumping?
} Is there much difference in our application for turbo molecular pumps that are oil-lubricated vs magnetic levitation bearings?
} Should we really be using a cold finger?
} Should we be venting the specimen airlock with dry nitrogen?
}
} Possible system 1:
} Rotary pump, 2x oil diffusion pumps
}
} Possible system 2:
} Scroll pump, turbo molecular pump
}
} Possible system 3:
} Diaphragm pump, turbo molecular pump, large IGP on column, small IGP on gun.
}
} Best regards,
}
} Ben
}
} --
} Research Support Manager (Imaging and IT Systems)
} MRC Brain Network Dynamics Unit,
} University of Oxford Department of Pharmacology,
} Mansfield Road, Oxford, OX1 3TH, United Kingdom.
} Telephone: 01865 271897 {http://www.mrcbndu.ox.ac.uk/}
}
Dear Ben,
We had a Techni 12 for scanning grids and doing routine work when I managed the facility at Caltech. The scope was the most reliable and trouble-free I’ve worked with. I must also add the disclaimer that I worked for about a year for FEI. The vacuum system that came with the scope was excellent, and it included ion pumps. I definitely recommend a cold finger. When the beam hits a resin specimen, organic fragments are produced that a cold finger will trap. This will prevent the column from getting contaminated and give you longer trouble-free service. I do not think you need to vent the airlock with nitrogen, but I was working in a place where the air was usually very dry. If Oxford is humid, dry nitrogen may be an advantage. Since the airlock has a small volume, a nitrogen tank should last a very long time and not be very expensive.
Yours,
Bill





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From: percius-at-lbl.gov
Date: Thu, 7 Sep 2017 14:01:53 -0500
Subject: [Microscopy] Frontiers of Electron Tomography Workshop and Short Course in

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Please join us for the first “International Workshop and Short Course
on the Frontiers of Electron Tomography in the Physical Sciences”
(www.electron-tomo.com) at Berkeley, CA, USA, on Oct. 23 - 26,
2017.This four-day event will consist of a two-day scientific workshop
on advanced electron tomography with invited and contributed talks
followed by a two-day short course on nanometer to atomic-resolution
electron tomography for graduate and postdoc level scientists.

--- Registration deadline is September 15th, 2017 ---

Please visit the conference website to register for the workshop,
submit a contributed talk/poster or request to attend the short
course. Space is especially limited for the short course so please
register as soon as possible if you are interested to attend.

The two-day workshop will bring together leading figures in electron
tomography and related fields to disseminate results and exchange
ideas. Topics to be covered include:

- Atomic Electron Tomography (AET)
- Electron Tomography at the Nanoscale
- Electron Holography
- Scanning Confocal Electron Microscopy
- Other 3D Imaging / Diffraction Methods
- Applications in the Physical Sciences

We expect about 100 participants to attend this workshop, which will
consist of invited talks, contributed talks and posters.

The subsequent Short Course is designed to teach the basic theory,
implementation and application of electron tomography for nanometer-
and atomic-resolution structural analysis. We will cover:

- Electron tomography theory
- Tilt-series pre-processing
- Basic and advanced reconstruction algorithms
- Three-dimensional data analysis and visualization

The goal of the short course is to train graduate students and
postdocs to be the future leaders in the field of high-resolution
electron tomography and to pursue its broad applications across
different disciplines.

Best regards,

Peter Ercius, Mary Scott, Colin Ophus, & John Miao

Members of the Organizing Committee of FET 2017


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From: bicarbaj-at-mtholyoke.edu
Date: Fri, 8 Sep 2017 10:49:53 -0500
Subject: [Microscopy] NESM Fall Meeting Registration OPEN

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello everyone,

The New England Society for Microscopy invites you to join us at JEOL
in Peabody, MA for our annual fall meeting on Sept 28th.

To learn more about our speakers and to register, please visit our
website at http://nesmicroscopy.org/upcoming-meetings/.

We hope to see you there!

Cheers,
-Blanca
2017 NESM President

-----------------------------------------
Blanca Carbajal Gonzalez, M.S.
Director of Microscopy
Science Center
50 College St
Mount Holyoke College
Clapp Laboratory 123
Office: 413-538-3118
Cell: 559-905-7138
bicarbaj-at-mtholyoke.edu
MHC Microscopy

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From: microscopy.listserver-at-gmail.com
Date: Sun, 10 Sep 2017 23:41:01 -0500
Subject: [Microscopy] Fwd: Re: viaWWW:air table for ultramicrotome

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu}

Jen,

Do you have partially-deflated (old) tennis balls, raquet balls, or the
like between the base of your granite table and the top?
That should help at least with the vibration.

If not … maybe a magician who knows how to levitate things? Or an air table.

Phil
------------- Philip Oshel Imaging Facility Director
Biology Department
1304 Biosciences
1455 Calumet Ct.
Central Michigan University
Mt. Pleasant, MI 48859
989 774-3576 office
989 774-7567 lab





Email: Jen.Fendler-at-utas.edu.au Name: Jen Fendler

Organization: University of Tasmania

Title-Subject: [Filtered] air table for ultramicrotome

Message: Dear TEM experts

Are there any cons to purchasing an air table rather than a passive
antivibration table for an ultramicrotome?

Our granite table is inadequate most days (although it was built pseudo
in-house rather than commercial purpose built for ultramicrotome, and
was sufficient before major hospital renovations nearby), and our
situation is that our TEM facility is on the 5th floor, and we have
numerous major ongoing building works on the blocks adjacent to our
building, so I doubt even a move to the basement would help for the next
1-2 years.

I wondered why air tables are not commonly used for ultramicrotomes and
if there's any rationale against this, and would like to know before
purchasing one. I'm trialing one at the moment and it works well (other
than minor annoyance of how easily it is bumped by the slightest brush
of eyes on oculars etc)

Kind regards
Jen



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From: John.Mardinly-at-asu.edu
Date: Mon, 11 Sep 2017 09:39:48 -0500
Subject: [Microscopy] Re: viaWWW:air table for ultramicrotome

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

What in the world is your TEM doing on the 5th floor? How do you handle vibration for the TEM?
A. John Mardinly, Ph.D., P.E.
Retired, ASU

}
} Message: Dear TEM experts
}
} Are there any cons to purchasing an air table rather than a passive
} antivibration table for an ultramicrotome?
}
} Our granite table is inadequate most days (although it was built pseudo
} in-house rather than commercial purpose built for ultramicrotome, and
} was sufficient before major hospital renovations nearby), and our
} situation is that our TEM facility is on the 5th floor, and we have
} numerous major ongoing building works on the blocks adjacent to our
} building, so I doubt even a move to the basement would help for the next
} 1-2 years.
}
} I wondered why air tables are not commonly used for ultramicrotomes and
} if there's any rationale against this, and would like to know before
} purchasing one. I'm trialing one at the moment and it works well (other
} than minor annoyance of how easily it is bumped by the slightest brush
} of eyes on oculars etc)
}
} Kind regards
} Jen
}
}
}
} ==============================Original Headers==============================
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From: oshel1pe-at-cmich.edu
Date: Fri, 15 Sep 2017 06:57:20 -0500
Subject: [Microscopy] Sources for temperature-controlled stages for gas adsorption studies

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Email: researchers4u-at-gmail.com Name: S. Shobeikeh

Organization: SHIRAZ university

Title-Subject: [Filtered] ultrasonic bath vs probe sonicator
Message: Dear colleagues:
We recently decided to purchase a sonicator for our TEM laboratory so we
can use it to disperse nanoparticles (like: CNT, Au, Ag, ZnO and so on)
in aqueous mediums. As you all know some laboratories use ultrasonic
bath and others use probe sonicator. I was wondering based on your
experience which of the above mentioned sonicators give the best result
for dispersion of nanoparticles. Any input would be appreciated.
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Name:Avinash Both
School:University of Nebraska-lincoln
Grade/Education Level:Graduate
Location:Lincoln, NE US
Email:avinash.both-at-huskers.unl.edu

Subject:Temperature controlled Stage for Microscope

Your Question:I want to do study adsorption of gases by nanomaterials using microscopy. For carrying out this experiment I need a temperature controlled sample stage where I can monitor the temperature as well ensure that there is no leakage. Could you please help me with suggestions in what kind of temperature controlled stage should I use for doing this study ? Thanks in advance.




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7, 59 -- Subject: Sources for temperature-controlled stages for gas adsorption studies
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From: oshel1pe-at-cmich.edu
Date: Fri, 15 Sep 2017 07:01:29 -0500
Subject: [Microscopy] Sources for temperature-controlled stages for gas adsorption studies

Contents Retrieved from Microscopy Listserver Archives
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***********************************************************************************
Forwarded from "Ask a Microscopist"
Please remember that the person asking the question is likely
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***********************************************************************************

Name:Avinash Both
School:University of Nebraska-lincoln
Grade/Education Level:Graduate
Location:Lincoln, NE US
Email:avinash.both-at-huskers.unl.edu

Subject:Temperature controlled Stage for Microscope

Your Question:I want to do study adsorption of gases by nanomaterials using microscopy. For carrying out this experiment I need a temperature controlled sample stage where I can monitor the temperature as well ensure that there is no leakage. Could you please help me with suggestions in what kind of temperature controlled stage should I use for doing this study ? Thanks in advance.




==============================Original Headers==============================
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7, 62 -- Subject: Sources for temperature-controlled stages for gas adsorption studies
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From: donc-at-asmicro.com
Date: Sat, 16 Sep 2017 09:55:20 -0500
Subject: [Microscopy] RTV silicone

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello:

I'm looking for a service engineer that specializes servicing vintage Zeiss 10 TEM's in the Northeastern USA. Please contact me off list.

Cheers!

Al Coritz
Applications & Service Manager
Electron Microscopy Sciences
1560 Industry Road
Hatfield, PA 19440
acoritz-at-emsdiasum.com
800-523-5874






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From nyshin-at-daelimcorp.co.kr Fri Sep 15 08:44:42 2017
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I have heard that Dow Corning #3140 RTV Silicone glue can be thinned to
paintable consistency with acetic acid(vinegar). It can then be applied as
a very thin coating to provide a water-tight seal in a small space.
What is the recipe to thin the glue?

regards,
Don
=============================================
Don Chernoff, Ph.D., President
Advanced Surface Microscopy, Inc.; 3250 N. Post Rd., Suite 120;
Indianapolis IN 46226 USA
E-Mail: donc-at-asmicro.com www.asmicro.com
Voice: +1-317-895-5630 Toll free: +1-800-374-8557 (in USA & Canada)
Fax: +1-317-895-5652
[business activities since 1990: analytical services in AFM, AFM probes,
consulting, training, calibration and test specimens,
calibration and measurement software, used NanoScope equipment.]
=============================================



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From: microscopy.listserver-at-gmail.com
Date: Sun, 17 Sep 2017 06:27:00 -0500
Subject: [Microscopy] viaWWW:High Resolution Display on Cambridge S-360

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Email: rim-at-drumagog.com Name: Rim Buntinas

Title-Subject: [Filtered] High Resolution Display on Cambridge S-360

Message: Hi,

Does anyone have experience with adding a high resolution display to an S-360? Our SEM didn't come
with one standard, but I have a spare high resolution mixer board and the PLL box from another
scope. I tried installing them, and added another store card (4 total now), but I can't seem to get
an image. Any help would be appreciated!

I wonder if this option needs changes to the options file on the disk to make it work?

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From: microscopy.listserver-at-gmail.com
Date: Sun, 17 Sep 2017 06:27:51 -0500
Subject: [Microscopy] viaWWW: hyperspectral microscopy

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Email: chrismillertx89-at-gmail.com Name: Chris Miller

Organization: UC Davis

Title-Subject: [Filtered] hyperspectral microscopy

Message: Does anyone know the cost of this hyperspectral microscopy setup and more importantly what
microscopes it can be used with? Our lab is interested in whether it can be installed on microscopes
we already have.

http://cytoviva.com/wp-content/uploads/2017/01/Spec-Sheet-CytoViva-VNIR-Standard_r011017.pdf

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From: microscopy.listserver-at-gmail.com
Date: Tue, 19 Sep 2017 05:46:16 -0500
Subject: [Microscopy] viaWWW:TEM imaging of Metals

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Chris

You will need to define what you mean by hyperspectral microscopy.

That is a spectroscopic data acquisition technique and depends upon
the excitation source and the detected signal (light, x-rays, electrons...)

I use electrons in TEM/STEM and it is possible to measure light, x-rays, and electrons,
but others wil use as the source SEM, X-rays , Ions, and least we not forget visible photons.


Nestor

===========================================
Dr. Nestor J. Zaluzec
Argonne National Laboratory
Photon Sciences Division
9700 S. Cass Ave
Bldg 212 / A-143
Argonne, Illinois 60439 USA
Email: Zaluzec-at-aaem.amc.anl.gov


Tel: 530-NES-TORZ (530-637-8679) has Voice Mail
Lab: 630-252-7901
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iChat: Zaluzec-at-AIM.com
Skype: Zaluzec
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Senior Scientist - Argonne National Laboratory
Fellow of the Microscopy Society of America
Senior Fellow the Computational Institute - University of Chicago
E.P. Wigner Fellow - Oak Ridge National Laboratory
Past President Microscopy Society of America
Adjunct Professor of Physics - Northern Illinois University &
the University of Illinois at Chicago
Visiting Professor of Microscopy - Manchester University

===========================================
TPMLab: http://tpm.amc.anl.gov
MMSite: http://www.amc.anl.gov
===========================================

The box said ...
"This program requires Win 95/98/NT or better..."
So I bought a Mac !

===========================================
LLAP
=========================================+=====



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From ronaldfaust205xw-at-gmail.com Sun Sep 17 19:19:26 2017
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Nicklas,
Okay got it

MD

-----Original Message-----
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Sent: Saturday, September 16, 2017 3:19 PM
To: Michael Delannoy

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Email: Silverj-at-miamioh.edu Name: Joshua Silverstein

Title-Subject: [Filtered] TEM imaging of Metals

Message: I am reaching out to the community to initiate a collaboration with a facility capable of
preparing alloy materials for TEM imaging. My facility does not have a dual beam, FIB or
electro-chemical polisher. Any of these would be sufficient. I am in Cincinnati, Ohio. Contact me at
silverj-at-miamioh.edu.
Cheers,
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From: microscopy.listserver-at-gmail.com
Date: Wed, 20 Sep 2017 05:57:06 -0500
Subject: [Microscopy] viaWWW:Advances in In-Situ Microscopy Workshop Drexel University

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Email: adjerid-at-vt.edu Name: Khaled Adjerid

Organization: Virginia Tech

Title-Subject: [Filtered] Modulus Calculation

Message: Hello Everyone,
I was wondering if anyone had an efficient way of taking large amounts of AFM indentation data and
quickly calculating modulus (either a matlab script or with Bruker software). I am running a Veeco
Bioscope II AFM and taking indents on soft materials (~0.5 gPa). I have 100's of files with Z height
and deflection error (Volts) I would like to process and curve fit using the Sneddon-Hertz cone
model is tedious to do on each individual file.
I have attempted to contact Bruker but they have not gotten back to me either. Is their software
capable of doing such a calculation?
Best,
Khaled Adjerid Virginia Tech
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Title-Subject: [Filtered] Advances in In-Situ Microscopy Workshop

Message: Addressing In-Situ TEM Challenges Using Integrated Hardware and Software

Drexel University, Bossone Research Center, Philadelphia , PA
8:00 am - 5:00 pm, Wednesday, October 4, 2017

This intensive workshop at Drexel University, Bossone Research Center, will present and demonstrate
state-of-the-art instruments and software for in-situ EM applications. Lectures and hands-on lab
sessions will show how a complete in-situ system can optimize data management and reveal critical
details in reaction processes. Specifically, temperature induced transistions in both complex oxide
materials and 2-dimentional MXene will be studied. Chemical and electronic transistions will be
measured with direct detection EELS and correlated with the sample IV behavior.

The workshop is complimentary to all confirmed registrants. Space is limited. Please register by
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From: peter-at-analyticslounge.org
Date: Wed, 20 Sep 2017 11:38:54 -0500
Subject: [Microscopy] Decomissioned TEM / Siemens Elmiskop 102

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,

There is a TEM (Siemens Elmiskop 102) being decommissioned in Chicago. Its operating status is not known, however, the unit represents historical value as I understand both due to the research that it made possible in addition to its age.

Whether it could be brought online again is another question entirely -- such an endeavor would not be for the faint of heart. Perhaps the best outcome would be historical preservation in a museum or otherwise.

In any event, there is little time before the unit ends up disposed of. Even interest in components for someone operating a similar instrument would be a better fate than it is currently slated for. Additionally, there is little time left before the equipment is dismantled (approximately one week).

If you have any interested in the unit, please feel free to reach out via phone or email.

Regards,
Peter Zieba
312-285-3794

==============================Original Headers==============================
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==============================End of - Headers==============================




From: microscopy.listserver-at-gmail.com
Date: Thu, 21 Sep 2017 06:26:02 -0500
Subject: [Microscopy] Fwd: Asking for help for Ziess Libera TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Peter,

at some time I owned an Elmiskop 101 which is nearly the same like the
101. I still have lots of plans, special gaskets, O-rings, tubes, HV
cables etc.

I surely will not be for those faint of heart to get it up to vacuum
condition and align it but if anybody needs help doing so, ask me. There
are some O-rings where you might never think of, and they might be
broken, if the TEM is on air since some years.

It will take ca. two days to get the TEM on pallets (ca. 4 of them ;-) 
) and the cross weight will be ca. 4500 pounds...


Best wishes,

Stefan



Am 20.09.2017 um 19:02 schrieb peter-at-analyticslounge.org:
} ----------------------------------------------------------------------------
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} Hi all,
}
} There is a TEM (Siemens Elmiskop 102) being decommissioned in Chicago. Its operating status is not known, however, the unit represents historical value as I understand both due to the research that it made possible in addition to its age.
}
} Whether it could be brought online again is another question entirely -- such an endeavor would not be for the faint of heart. Perhaps the best outcome would be historical preservation in a museum or otherwise.
}
} In any event, there is little time before the unit ends up disposed of. Even interest in components for someone operating a similar instrument would be a better fate than it is currently slated for. Additionally, there is little time left before the equipment is dismantled (approximately one week).
}
} If you have any interested in the unit, please feel free to reach out via phone or email.
}
} Regards,
} Peter Zieba
} 312-285-3794
}
} ==============================Original Headers==============================
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} 6, 42 -- From: Peter Zieba {peter-at-analyticslounge.org}
} 6, 42 -- To: microscopy-at-microscopy.com
} 6, 42 -- Message-ID: {1239401350.41061.1505928787111.JavaMail.zimbra-at-analyticslounge.org}
} 6, 42 -- Subject: Decomissioned TEM / Siemens Elmiskop 102
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} 6, 42 -- Thread-Topic: Decomissioned TEM / Siemens Elmiskop 102
} ==============================End of - Headers==============================

--
-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.nanoflight.info
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www.elektronenmikroskopie.info
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www.assisi.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------


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From watkinsmelanie58niryh-at-gmail.com Thu Sep 21 00:46:15 2017
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X-from: Shahrzad Hosseini {shahrzad.hosseini-at-gmail.com}

Hi All,

Is there anybody in this community having a Ziess Libera TEM? How do you get service for repairs and
maintenance form Ziess given that Ziess stops manufacturing TEMs?

Thanks,
Shahrzad

==============================Original Headers==============================
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From: microscopy.listserver-at-gmail.com
Date: Sat, 23 Sep 2017 07:56:06 -0500
Subject: [Microscopy] viaWWW:GWNIC Correlative Microscopy Workshop

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Email: mjaklewi-at-yorku.ca Name: Magdalena Jaklewicz

Organization: York University

Title-Subject: [Filtered] TEM coated grids

Message: Hello Microscopists:

does anybody collect images of unsuccessfully coated grids?
I have polymeric substrate carbon activated grids (purchased) - I have difficulty to understand what
is on them... looks like I do not even need a specimen, there is plenty to analyze as it is .... I
will be returning them - but I would love to take a look at some typical (and atypical) failures
during the grid preparation,

have a lovely day

Magdalena




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Email: chrisbrantner-at-gwu.edu Name: Chris Brantner

Organization: The George Washington University

Title-Subject: [Filtered] GWNIC Correlative Microscopy Workshop

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From: microscopy.listserver-at-gmail.com
Date: Sat, 23 Sep 2017 08:01:19 -0500
Subject: [Microscopy] Fwd: Asking for help for Ziess Libera TEM

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X-from: Shahrzad Hosseini {shahrzad.hosseini-at-gmail.com}




Hi All,

Is there anybody in this community having a Ziess Libera TEM? How do you get service for repairs and
maintenance form Ziess given that Ziess stops manufacturing TEMs?

Thanks,
Shahrzad

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From: mike.marko.em-at-gmail.com
Date: Sat, 23 Sep 2017 13:49:24 -0500
Subject: [Microscopy] Decomissioned TEM / Siemens Elmiskop 102

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Stefan and Peter,

I also had one. It was an excellent TEM. I also still have some parts,
including the desk/panel units and the complete roll-out pumping system.

You should contact Alan Hawk (alan.j.hawk.civ-at-mail.mil) at the National
Museum of the History of Medicine, which is located in Silver Spring, MD.

NMHM has one of nearly every Siemens TEM in their collection (among many
other TEMs), and Alan might be interested.

BTW: I have three Siemens Elmiskop TEMs, one of which is a Ia from 1964,
and is back in operating condition. I ask the list if anyone knows of
another Siemens TEM still in operation.

Mike Marko
MSA Archivist

On 9/20/2017 1:29 PM, diller-at-stefan-diller.com wrote:
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}
} Hi Peter,
}
} at some time I owned an Elmiskop 101 which is nearly the same like the
} 101. I still have lots of plans, special gaskets, O-rings, tubes, HV
} cables etc.
}
} I surely will not be for those faint of heart to get it up to vacuum
} condition and align it but if anybody needs help doing so, ask me. There
} are some O-rings where you might never think of, and they might be
} broken, if the TEM is on air since some years.
}
} It will take ca. two days to get the TEM on pallets (ca. 4 of them ;-)Â
} ) and the cross weight will be ca. 4500 pounds...
}
}
} Best wishes,
}
} Stefan
}
}
}
} Am 20.09.2017 um 19:02 schrieb peter-at-analyticslounge.org:
} } ----------------------------------------------------------------------------
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} }
} } Hi all,
} }
} } There is a TEM (Siemens Elmiskop 102) being decommissioned in Chicago. Its operating status is not known, however, the unit represents historical value as I understand both due to the research that it made possible in addition to its age.
} }
} } Whether it could be brought online again is another question entirely -- such an endeavor would not be for the faint of heart. Perhaps the best outcome would be historical preservation in a museum or otherwise.
} }
} } In any event, there is little time before the unit ends up disposed of. Even interest in components for someone operating a similar instrument would be a better fate than it is currently slated for. Additionally, there is little time left before the equipment is dismantled (approximately one week).
} }
} } If you have any interested in the unit, please feel free to reach out via phone or email.
} }
} } Regards,
} } Peter Zieba
} } 312-285-3794
} }
} } ==============================Original Headers==============================
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From: microscopy.listserver-at-gmail.com
Date: Tue, 26 Sep 2017 05:59:12 -0500
Subject: [Microscopy] viaWWW:Embedding powder for SEM - alternatives to epoxy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Shahrzad,

As a general rule, once the OEM drops support of your instrument for one reason or another you go to one of third-party support organizations. Try reaching out to service engineers who were servicing your instrument while it was supported - even if they are not allowed to help you, they could know who can. Network with used equipment dealers and speak to other TEM users in your region to find out support options in your geographic location. Google for similar instrument around the world and contact tool owners to find out where they obtain support. You could also try posting similar request in relevant LinkedIn user groups.

Keep in mind that decent third-party service is *not* cheaper then OEM support. Depending on your geographical location and also age, condition, and degree of neglect of your
instrument, cost of T&M service call for bringing it back to service could be out of reach...

Good luck!

Valery Ray
===========================
PBS&T, MEO Engineering Company
290 Broadway, Suite 298
Methuen, MA 01844, USA
Phone: +1-978-305-0479 - leave a message
Mobie: +1-978-305-0479 - leave a message
E-mail: vray-at-partbeamsystech.com
Web: www.partbeamsystech.com
Web: www.freudlabs.com

}   From: "microscopy.listserver-at-gmail.com"
} |--microscopy.listserver-at-gmail.=
} com--|
} To: vray-at-partbeamsystech.com=20
} Sent: Saturday, September 23, 2017
} 10:07 AM
} Subject: [Microscopy] Fwd: Asking for
} help for Ziess Libera TEM
}   =20
}
}
}
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} X-from: =C2=A0=C2=A0=C2=A0 Shahrzad
} Hosseini |--shahrzad.hosseini-at-gmail.com--|
}
}
}
}
} Hi All,
}
} Is there anybody in this community
} having a Ziess Libera TEM? How do you ge=
} t service for repairs and=20
} maintenance form Ziess given that Ziess
} stops manufacturing TEMs?
}
} Thanks,
} Shahrzad


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Name: Pl Baggethun

Organization: Elkem

Title-Subject: [Filtered] Embedding powder for SEM - alternatives to epoxy

Message: Purpose: Embed powders for studies in plane section by SEM. Powder particles range in size
typically 1 um to 100 um.

Problem: Standard epoxy for metallographic specimen preparation has high basicity and reacts with
some acidic mineral powders such as Si.
Question: Are there alternatives to epoxy that are also viscous enough to make random sections, that
is, the particles must not be allowed to migrate in the resin after mixing?

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From: microscopy.listserver-at-gmail.com
Date: Tue, 26 Sep 2017 06:01:44 -0500
Subject: [Microscopy] Fwd: Re: Fwd: Asking for help for Ziess Libera TEM

Contents Retrieved from Microscopy Listserver Archives
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X-from: Juan Luis Ribas {jlribas-at-us.es}



Hello Shahrzad,

In Spain, Zeiss is still giving us support. Indeed, they have to do it at least till 2024 since we
bought the last unit a few years ago.

Sometimes you can get support from the community, so if you have some problem you can post it here.

Best regards

Juan Luis




El 23/09/2017 a las 15:18, microscopy.listserver-at-gmail.com escribió:
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} X-from: Shahrzad Hosseini {shahrzad.hosseini-at-gmail.com}
}
}
}
}
} Hi All,
}
} Is there anybody in this community having a Ziess Libera TEM? How do you get service for repairs and
} maintenance form Ziess given that Ziess stops manufacturing TEMs?
}
} Thanks,
} Shahrzad
}
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From: microscopy.listserver-at-gmail.com
Date: Tue, 26 Sep 2017 06:02:23 -0500
Subject: [Microscopy] Fwd: Lens alignment on a Cambridge S-360 SEM

Contents Retrieved from Microscopy Listserver Archives
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X-from: Rim Buntinas {rim-at-drumagog.com}


Hi,

Does anyone have information on how to align the lenses on a Cambridge S-360? We just starting
using this SEM and would love to get the column aligned. It seems that Optibeam isn’t doing what it
should and I wonder if the condenser lenses need alignment. I appreciate the help!

Rim

==============================Original Headers==============================
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From: brachfelds-at-mail.montclair.edu
Date: Wed, 27 Sep 2017 19:49:06 -0500
Subject: [Microscopy] Assistant professor position in Paleoclimatology/Geochemistry

Contents Retrieved from Microscopy Listserver Archives
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Dear Colleagues,
Please share the enclosed position announcement with anyone you know
who may be interested.
Thank you,
Stefanie

Assistant professor position in Paleoclimatology/Geochemistry

The Department of Earth and Environmental Studies at Montclair State
University (New Jersey) invites applications for a full-time
(10-month) tenure-track Assistant Professor position in
Paleoclimatology/Geochemistry. We seek individuals who work with
geological archives (corals, speleothems, ocean sediments, lake
sediments, ice cores, glacial deposits, tree rings) to understand
Earth’s climate processes such as atmospheric and ocean circulation,
the global carbon cycle, cryosphere dynamics and how these processes
affect biological and/or human systems, over a range of time scales.
The successful candidate will develop a vigorous externally funded
research program, engage in student mentoring, and have a strong
commitment to excellence in teaching. The candidate will play a
critical role in our bachelors, masters, and doctoral programs in
Earth and Environmental Science, Geography, Sustainability Science,
and Environmental Management. Teaching responsibilities will include
introductory courses in Earth and Environmental Science, as well as
upper-level undergraduate and graduate courses within the applicant’s
areas of expertise. Service to the department, university, and larger
professional community is required.

Qualifications & Requirements
Applicants are expected to have a Ph.D. in Earth and Environmental
Science, Earth System Science, Geology, Geochemistry, Geography, or
other appropriate field, a record of peer-reviewed scholarship in
paleoclimatology and geochemistry, and evidence of potential for
success in grant activity. Post-doctoral experience is desirable.

Anticipated Start Date: September 1, 2018
Apply: Electronic applications assembled into one combined .pdf or
.doc file, and containing a cover letter, CV, statements of teaching
and research interests, and the names and contact information for
three professional references should be sent to
EAESsearch-at-montclair.edu. Please include position number V-F24.

Apply By: October 20, 2017


*********************
Dr. Stefanie Brachfeld
Acting Associate Dean for Academic Affairs, College of Science and Mathematics
Montclair State University
Montclair, NJ 07043
phone: (973) 655-5129
brachfelds-at-mail.montclair.edu
*********************


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From: xinran.liu-at-yale.edu
Date: Fri, 29 Sep 2017 11:51:01 -0500
Subject: [Microscopy] Fast scan TEM camera

Contents Retrieved from Microscopy Listserver Archives
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Dear Colleagues,

I am looking for a solution to replace our Olympus Morada camera on a Tecnai Biotwin (80kV). We have been happy with this camera for past 10 years, but the CCD is deteriorating and there is no hard/software support available any more.

It would be very helpful if you can let me know your thoughts on similar kind of cameras currently available on the market with reasonable price. Users are cell biologists, so a large CCD chip and fast searching mode is necessary, the high resolution is not required. I am also thinking to add a similar one on our Tecnai F20 for the similar purpose.

Thanks very much in advance.

Xinran N. Liu
________________________________________

Xinran Nick Liu, M.D. & Ph.D.
Director of Center for Cellular & Molecular Imaging
Bio & Cryo Electron Microscopy Core Facility
Yale University School of Medicine
Office: 203.785.4050
Lab: 203.785.5390
http://medicine.yale.edu/ccmi/em




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9, 70 -- Subject: Fast scan TEM camera
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From: microscopy.listserver-at-gmail.com
Date: Sat, 30 Sep 2017 07:16:39 -0500
Subject: [Microscopy] Fwd: Lens alignment on a Cambridge S-360 SEM

Contents Retrieved from Microscopy Listserver Archives
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X-from: alan stone {alan.astonmet-at-gmail.com}



Hi Rim,

I've been running my Cambridge S200 (pretty much the same column) for almost 30 years. If the
instrument has been sitting for a while, you should pull out, disassemble and thoroughly clean the
column liners, gun assembly and apertures. The alignment is a repetitive "tweaking" of aperture
position and electronic alignment controls throughout a wide range of current settings.

We had an old S360 just for parts. We may have some circuit boards that we were going to dispose of.
If we still have them, they are yours for the taking.



Al Stone
WJE

*Alan Stone**
*Principal

*Wiss, Janney, Elstner Associates, Inc. ***
/Engineers /|/Architects / |/Materials Scientists/
200 Larkin Drive, Suite A, Wheeling, Illinois 60090

tel 847.353.8100 | fax 847.353.8204
direct 847.753.6352 | mobile 847.636.1300
www.wje.com {http://www.wje.com/} __

_astone-at-wje.com {mailto:astone-at-wje.com} _

*/Formerly with ASTON Metallurgical Services Company/*
www.astonmet.com {http://www.astonmet.com}

This confidential message is for the private use of the recipient as shown. If this has been
delivered to you in error, then please return the message and delete from your files. Thank you.


On Tue, Sep 26, 2017 at 6:03 AM, {microscopy.listserver-at-gmail.com
{mailto:microscopy.listserver-at-gmail.com} } wrote:




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X-from: Rim Buntinas {rim-at-drumagog.com {mailto:rim-at-drumagog.com} }


Hi,

Does anyone have information on how to align the lenses on a Cambridge S-360?  We just starting
using this SEM and would love to get the column aligned.  It seems that Optibeam isn’t doing
what it
should and I wonder if the condenser lenses need alignment.  I appreciate the help!

Rim

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From: microscopy.listserver-at-gmail.com
Date: Wed, 4 Oct 2017 05:58:13 -0500
Subject: [Microscopy] viaWWW:How to deblur STEM images

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Email: tom.strader-at-microscopyinnovations.com Name: Tom Strader

Organization: Microscopy Innovations

Title-Subject: [Filtered] Midwest Microscopy & Microanalysis meeting Oct. 13th at MCW

Message: Topics in BioMedical Microscopy

Presented by:
Midwest Microscopy and Microanalysis Society (M3S)
A local affiliate of the Microscopy Society of America and the Microanalysis Society

Hosted by the Medical College of Wisconsin

Friday, October 13th, 2017
Medical College of Wisconsin
8701 W Watertown Plank Rd, Milwaukee, WI 53226
Meeting location: Building A
Parking is free
Located in Visitor's lot

Onsite Registration Fee: Meeting Free for M3S members, $20.00 for non-members, $5.00 for students
(Fee includes M3S membership for 2017/2018)
Please RSVP by Tuesday, October 10th to: Secretary-at-midwestmicroscopy.org

*********************************************

8:00 – 8:45a Registration
9:00 – 9:10 a Welcome and Opening Remarks
9:10 – 9:55a Recent advances in confocal imaging (SIM, TIRF) including tissue clearing & co-localization
Robert Price - Research Professor of Cell Biology & Anatomy, Director of the Instrumentation
Resource Facility (IRF), University of South Carolina School of Medicine.
(http://dba.med.sc.edu/price.asp)
9:55 – 10:40a Cryo-EM tomography of RNA virus genome replication organelles
Paul Ahlquist - Paul J. Kaesberg Professor of Molecular Virology and Oncology at the University of
Wisconsin, Madison, and John and Jeanne Rowe Chair in Virology at the Morgridge Institute for
Research. (http://www.hhmi.org/scientists/paul-ahlquist)
presented by
10:40 – 11:10a Break – Visit with Vendors
11:10 – 11:55a - Multiscale imaging of the cellular microenvironment
Kevin Eliceiri - Director, Laboratory for Optical and Computational Instrumentation (LOCI Center) UW
Madison (https://loci.wisc.edu/people/kevin-eliceiri)
11:55 - 1:10p Lunch – Visit with Vendors
1:10 – 1:30p Microtubules and the ciliary photoreceptor
Tylor Lewis - Graduate Student, Medical College of Wisconsin, Department of Ophthalmology & Visual
Sciences
1:30 – 1:50p Imaging and quantifying mitochondrial morphology
Megan C. Harwig - Research Scientist, Medical College of Wisconsin, Department of Biochemistry
1:50 – 2:10p In vivo and ex vivo imaging of the ground squirrel retina
Benjamin S. Sajdak - Graduate Student, Medical College of Wisconsin, Department of Neuroscience,
Advanced Ocular Imaging Program
2:10 – 2:30p Total Internal Reflection Fluorescence Microscopy - TIRF
Chen Chen - Postdoctoral Fellow, Medical College of Wisconsin, Barbieri Lab

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Email: rz2371-at-columbia.edu Name: Rui Zu

Organization: Columbia University

Title-Subject: [Filtered] How to deblur STEM images

Message: Hi, all. I got the STEM images from Talos F200X, the parameters of images are 200kv,
condenser aperture 50, 10Mx, condenser lens 205mm, spot size 9, acquisition time 20s. I have tried
low-pass filter, and currently, I am coding wiener deconvolution in MATLAB, but it requires Point
Spread Function. How do you estimate the point spread function or how to make your images clearer?
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From: microscopy.listserver-at-gmail.com
Date: Wed, 4 Oct 2017 06:14:22 -0500
Subject: [Microscopy] Fwd: Annual Meeting Michigan Microscopy & Microanalysis Society and

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X-from: John Mansfield {thejfmjfm-at-me.com}

Announcing:

The Annual Meeting of Michigan Microscopy & Microanalysis Society and Michigan Center for Materials
Characterization
Open House
Wednesday, November 1st 2017

Abstracts may be submitted via:
http://www.michmicroscopy.org/paper.php
Deadline for submission: September 29th, 2017 (now extended to October 10th 2017)

Location:
University of Michigan
North Campus ResearchComplex, Dining Hall
Building 18, 2800 Plymouth Rd,
Ann Arbor MI 48109-2800

Schedule:
8:30 - 9:00 Registration and breakfast
9:00 - 9:05 Welcome and introduction
9:05 - 10:35 Keynote speakers
10:50 - 12:20 Contributed presentations 12:20 - 14:30 Luncheon
Guided tours of (MC)2, poster session, micrograph contest, visiting vendors
14:30 - 16:45 Further presentations 16:45 - 17:00 Poster and micrograph competition awards
17:00 - 17:30 MMMS Business meeting

Keynote presentations include:

Determining atomic structure across scale & dimensions with highly convergent electron beams
Prof. Robert Hovden, Materials Science and Engineering, University of Michigan

Using cryo-electron microscopy to visualize the function of PMP22, a major cause of
Charcot-Marie-Tooth disease (CMTD)
Prof. Melanie Ohi, Life Sciences Institute, University of Michigan
Shaping (and reshaping) biological membrane architecture for vertebrate photoreceptor health (and
disease)
Prof. Andrew Goldberg, Biomedical Sciences, Oakland University

Ultrasonic Fatigue and its role in Materials Centric Design
Dr. Jason W. Carroll, Eaton Corporation

For details and questions contact John Mansfield, jfmjfm-at-umich.edu
____
John Mansfield PhD Cphys MInstP
Director of Education & Engagement
Michigan Center for Materials Characterization
Building 28, Room 3045W, University of Michigan
2800 Plymouth Road, Ann Arbor MI 48109-2800 USA
Phone: (734) 936-3352 Cell. Phone: (734) 834-3913
Email: jfmjfm-at-umich.edu


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13, 54 -- Subject: Fwd: Annual Meeting Michigan Microscopy & Microanalysis Society and
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From: microscopy.listserver-at-gmail.com
Date: Wed, 4 Oct 2017 06:20:31 -0500
Subject: [Microscopy] Jacques Dubochet, Joachim Frank and Richard Henderson win the 2017

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For those few that might not have heard the news late yesterday
The Nobel Prize in Chemistry was awarded to

Jacques Dubochet, Joachim Frank and Richard Henderson

for their work in Cryo-EM

http://www.cnn.com/2017/10/04/world/nobel-prize-chemistry-2017-cryo-electron-microsopy/index.html


On behalf of the Microscopy Listserver community, I will offer Congratulations to them from all
of us, a well deserved honor.

Your Friendly Neighborhood SysOp

Nestor


===============================================

Do not reply to this messages it is from
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From: stefan.diller-at-t-online.de
Date: Thu, 5 Oct 2017 09:22:12 -0500
Subject: [Microscopy] FEG-Imaging of inkjet dyes

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Dear All,

I would like to image water-based pigment dyes for inkjet printing.

There might be some tricks to spread the dyes as thin as possible on a specially prepared glass substrate. Should I use
Polysine-coated slides or will the Van der Waals forces to their thing on the nano-sized particles on every smooth surface?


Thanks,

Stefan


--


-----------------------------------------------------
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Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
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www.assisi.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------


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From: jacques.faerber-at-ipcms.unistra.fr
Date: Tue, 10 Oct 2017 02:16:42 -0500
Subject: [Microscopy] SEM detetctors acronyms !

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From johnsonloretta061loyiu-at-gmail.com Mon Oct 9 08:14:26 2017
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Hi all

I'm preparing some documentation for a SEM course and I want to give a
sort of glossary I begin some time ago, of "all" (or more modestly, a
typical sampling of...) SEM detector acronyms used on the different
manufacturers / machines, like Upper/Lower, Top, SE1/SE2, ETD, BSD,
Compo, etc.

It's sometime a puzzle for beginner (and trained people too) to find
what type of electrons does the YWK detector from the SEM-999 collect,
or what are the differences between the YWK-a and the YWK-u detector !

So may I ask if some SEM users could send me the following informations :
    -manufacturer, model, and age of your SEM
    -acronym of the different detectors avaible on it, with their full
meaning
    -the kind of signal they collect and  the type of informations they
give.

If I get such return for only 15-20 machines, and with what I've soon
collected, it can give a usefull list !

Thanks in advance, and I'll send a feadback when it is done.

Best regards
Jacques


--

J. Faerber
IPCMS-DSI
Institut de Physique et Chimie des Matériaux de Strasbourg
Département Surfaces et Interfaces
23, rue de Loess ; BP43
67034 Strasbourg CEDEX 2
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)3 88 10 72 48
E-mail : Jacques.Faerber-at-ipcms.unistra.fr


==============================Original Headers==============================
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From: microscopy.listserver-at-gmail.com
Date: Wed, 11 Oct 2017 08:30:52 -0500
Subject: [Microscopy] Fwd: Fwd: NESM 50th Anniversary special meeting at UMass, Amherst;

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Paul Palazzolo {paul-at-jpamri.com}


*Position Title:*Environmental Laboratory Manager

**

*Salary Range:*Commensurate with experience

*Location:*Greater Chicago Area

*Company Description:*This highly respected privately held, multi-disciplined environmental
laboratory testing firm with a national presence has been around for several decades, working with
high end, loyal clientele across the gamut of Fortune 500, industrial, commercial, institutions,
government, engineering and consulting firms.

*Job Description: *The core competencies would be the full gamut of Laboratory Management duties in
relation to managing, mentoring, developing programs, etc... that will drive the lab to its' highest
success.

This exciting opportunity combines the best of working with the resources of an established national
firm and the nimbleness of a privately held firm where you could still "put your stamp on it". **

**

This Laboratory Manager will:

*

Manage all aspects of the environmental laboratory operations, analysis, preparation, QA/QC, etc.

*

Preferred experience related to microbial, industrial hygiene, indoor air quality, asbestos and
Polarized Light Microscopy ( PLM )

*

Must be experienced with quality control, quality assurance (QA/QC) and daily operations of a
laboratory responsible for testing environmental samples

*

Supervise and train new staff

*

Ensure QA/QC policies and proceduresand compliance

*

Ensure data is high quality, clients are serviced, and lab operations are smooth and efficient

*

Conduct reviews and audits to ensure overall program efficiency which includes examination of
all testing data, policies and procedures

*

*Requirements: *BS in Life Sciences - Microbiology or Biology or related. Minimum 3 years of
supervisory experience with laboratory knowledge and 5 years of analytical experience


#

#

*

**

Sincerely,

Paul Palazzolo
Senior Managing Partner

/One of the nation's leading Environmental Search Firms/

//

(Toll Free) 866.712.1810

Paul-at-JPAmri.com {mailto:PaPaul-at-JPAmri.com}

www.JPAmri.com {http://www.JPAmri.com}

Invite me to LinkedIn at:

http://www.linkedin.com/in/paulpalazzolo

*
*P*
Please consider the environment before printing my e-mail***//**//**//*

*


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From trojlita384boyq-at-gmail.com Tue Oct 10 11:38:47 2017
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X-from: Wendy Salmon {wsalmon-at-wi.mit.edu}



Dear Fellow Microscopists,

New England Society of Microscopy is excited to announce our Special 50^th Anniversary of NESM
Fall Symposium and Workshops/Business Meeting, which will be held at the University of
Massachusetts, Amherst on November 8th and 9th, 2017.  Please find the linked flyer.

The symposium will be a two day program including workshops by Nikon on November 8th and technical
talks by New England researchers on the 9th.

Information about meetings details and registration can be found at:
http://nesmicroscopy.org/upcoming-meetings/
{https://nesmicroscopy.wildapricot.org/EmailTracker/LinkTracker.ashx?linkAndRecipientCode=jqqgwubZ1tB9%2bDl%2f3DEjKn7qMym9OYqCa9t6M21dagevHFmZs24uMCg2em%2fudFr9O9fJb%2bGzZ6yMYmGaRpQ68LBydqCFO%2b4RjSrsZqxxiVM%3d}

This year we have a great selection of technical talks and poster session.

If you have any questions regarding the poster session, please contact info-at-nesmicroscopy.org
{mailto:info-at-nesmicroscopy.org} .

We hope to see you in November at UMass, Amherst!

Cheers,

NESM Board

NESM fall symposium.pdf
{https://nesmicroscopy.wildapricot.org/EmailTracker/LinkTracker.ashx?linkAndRecipientCode=a0qQJ8xGDNKzPonorGjrw0we5nMSSzTWwxTu%2f8JHlVPRuj5%2bnFRFosetssaNe3keMh5%2bzHAqZIpH8ScfpDEhpzJl13geoBaGrn85FMhthg0%3d}





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From: microscopy.listserver-at-gmail.com
Date: Wed, 11 Oct 2017 08:32:31 -0500
Subject: [Microscopy] viaWWW: Postdoc position in Advanced Microscopy of Steel

Contents Retrieved from Microscopy Listserver Archives
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X-from: bassimn-at-mcmaster.ca

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Email: bassimn-at-mcmaster.ca Name: Nabil Bassim

Organization: McMaster University

Title-Subject: [Filtered] Postdoc position in Advanced Microscopy of Steel Microstructure and Thin Films

Message: A postdoctoral position is available in the performance of two distinct projects. The
researcher will use the state-of-the-art aberration-corrected TEM's at the Canadian Centre for
Electron Microscopy (ccem.mcmaster.ca) located at McMaster University, Hamilton Canada. The CCEM is
a world-class imaging facility and is equipped with 4 TEM's, including two aberation-corrected
microscopes (FEI Titans).
The first project is to study precipitation mechanisms of Nb-rich steels after hot rolling. This
work requires knowledge of chemical imaging, diffraction methods, and an understanding of strain
measurement. The second project is the study of the thin film growth process, from 2-D materials up
to ALD-grown films. This requires high-resolution imaging (TEM and STEM), EELS, and EDS.
The postdoc will be part of the Bassim research group, with a strong collaboration with Prof.
Zurob's research group.

Applicants should have a strong background in electron microscopy, preferably with
aberration-corrected (S)TEM experience. Additional background in metallurgy or thin films is a
bonus. Ability to work in a team, mentor graduate students, as well as excellent verbal and written
communication skills are expected.

McMaster University is located in Hamilton, Ontario, within a 45 minute drive from downtown Toronto,
which a world class city and 45 minute drive to Niagara Falls.

To apply, please send a cover letter, curriculum vitae including references, and 1-2 relevant
published papers to the e-mail below.

Nabil Bassim
Associate Professor
Department of Materials Science and Engineering, JHE 258
McMaster University
1280 Main Street West
Hamilton, ON L8S 4L8
CANADA
bassimn-at-mcmaster.ca

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From: frank_karl-at-ardl.com
Date: Wed, 11 Oct 2017 09:52:33 -0500
Subject: [Microscopy] Wollastonite in rubber

Contents Retrieved from Microscopy Listserver Archives
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Does anyone know a lab that want to be paid to look for wollastonite in rubber stock? We have a client that want a quote for this service. The problem, as it's been explained to me, is they want to know more than if there are particles of Ca silicate present. They want to know is it wollastonite. We don't have PLM or X-ray diffraction, so ARDL can't do it.

Any comments or suggestions would be helpful.

Thanks and advance!


Stay safe...........

Frank Karl
Microscopist
Akron Rubber Development Laboratory
2887 Gilchrist Road
Akron, Ohio 44305



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From: microscopy.listserver-at-gmail.com
Date: Fri, 13 Oct 2017 20:52:20 -0500
Subject: [Microscopy] viaWWW:wearing a pacemaker while using field Emission SEMs or FIBs?

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Email: pscallio-at-dal.ca Name: Pat Scallion

Organization: Dalhousie University

Title-Subject: [Filtered] wearing a pacemaker while using field Emission SEMs or FIBs

Message: Hello all,

I was wondering what experience you have had, of a user with an MRI compatible pacemaker and leads,
using and maintaining an electron microscope with ion pumps for the column vacuums? We have a
Hitachi S-4700 and a Hitachi FB-2000A from 2003.

Is it a big NO, can it be done with a distance from the system maintained, is there any shielding
that can be worn, or any other concerns that I am forgetting? What issues may be encountered?

Regards,
Pat Scallion

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From: colijn.1-at-osu.edu
Date: Sun, 15 Oct 2017 20:30:29 -0500
Subject: [Microscopy] viaWWW:wearing a pacemaker while using field

Contents Retrieved from Microscopy Listserver Archives
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Hi Pat,

I assume that you are worried about the user and not the imaging. The
magnetic fields around (i.e. outside) the instrument would come
primarily from the ion pumps and even those are pretty well contained.
Also any stray fields are static i.e. not time varying. The field
levels the user would encounter in an MRI *FAR* exceed what you will
find around an SEM plus they are repetitive so they would have a much
stronger effect on the user's pacemaker.

If anything I'd be more worried about the user affecting the imaging
rather than the SEM affecting the user.

Cheers,
Henk


--------------------

Hendrik O. Colijn
Center for Electron Microscopy and AnalysiS
The Ohio State University
1305 Kinnear Rd, Suite 100, Columbus, OH 43212

colijn.1-at-osu.edu 614/643-3458
cemas.osu.edu

"Time is that quality of nature which keeps things from happening all at
once." (Ray Cummings - 1922)
Lately it doesn't seem to be working.

------ Original Message ------
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To: colijn.1-at-osu.edu
Sent: 10/13/2017 9:54:10 PM



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13, 123 -- Subject: Re: [Microscopy] viaWWW:wearing a pacemaker while using field Emission SEMs or
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From: microscopy.listserver-at-gmail.com
Date: Mon, 16 Oct 2017 07:38:05 -0500
Subject: [Microscopy] viaWWW: Zeiss LSM5 Live module

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Email: leandro.lemgrubersoares-at-glasgow.ac.uk Name: Leandro Lemgruber

Organization: Wellcome Centre for Molecular Parasitology - University of Glasgow

Title-Subject: [Filtered] LSM5 Live

Message: Hi everyone

We are interested in a Zeiss LSM5 Live module for some in vivo imaging. Does anyone has this module
that we could purchase from? Or know where we could get one?

Thanks!

Leandro

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From: frank_karl-at-ardl.com
Date: Mon, 16 Oct 2017 12:11:06 -0500
Subject: [Microscopy] Thanks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I want to thank everyone who responded to my request for information on analysis and identification of wollastonite. I've forwarded the information and had a lot of fun deleting the out of town messages. Looks like I'll get to do that again.....


Stay safe...........

Frank Karl
Microscopist
Akron Rubber Development Laboratory
2887 Gilchrist Road
Akron, Ohio 44305



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From: microscopy.listserver-at-gmail.com
Date: Wed, 18 Oct 2017 15:05:00 -0500
Subject: [Microscopy] viaWWW: Imaging Specialist position open at NU

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

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Email: j-hornick-at-northwestern.edu Name: Jessica E Hornick

Organization: Biological Imaging Facility/Northwestern University

Title-Subject: [Filtered] Imaging Specialist position open at NU

Message: Hello,

The Biological Imaging Facility at Northwestern's Evanston campus is
currently hiring for an Imaging Specialist.

The job posting is available here:
https://careers.northwestern.edu/psp/hr92prod_er/EMPLOYEE/HRMS/c/HRS_HRAM.HRS_APP_SCHJOB.GBL?Page=HRS_APP_SCHJOB&Action=U&FOCUS=Applicant&SiteId=1

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From: microscopy.listserver-at-gmail.com
Date: Wed, 18 Oct 2017 15:06:03 -0500
Subject: [Microscopy] viaWWW:AVAILABLE: Vibration Isolation Platforms

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Lett.j.m-at-wustl.edu

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Email: Lett.j.m-at-wustl.edu Name: Jaclynn Lett

Organization: Washington University in St. Louis

Title-Subject: [Filtered] AVAILABLE: Vibration Isolation Platforms

Message: We have two TMC MICRO-g Series 65 vibration isolation tables
available immediately at no charge. They have already been dismantled in
order to remove the electron microscopes they supported. Photos and
other general information can be sent upon request.

Small table (custom-built with custom-placed supports)
-- Platform: 55 in x 35 in (3 in thick)
-- Four supports: each approximately 15 in x 7 in x 7 in

Large table (TMC MICRO-g model #65-29604-0)
-- Platform: 84 in x 56 in at widest points (5 in thick)
-- Four supports: each approximately 16 in x 9 in x 9 in

The recipient will be responsible for all moving and shipping costs. (We
can put you in contact with a company already familiar with the
equipment.) Removal is required by Wednesday, November 15th.

Please let me know of any interest. Thank you.

Jaclynn Lett Senior Research Technician
Department of Otolaryngology Washington University School of Medicine
4523 Clayton Avenue, Campus Box 8115 St. Louis, MO 63110 Office:
314-747-7257
Fax: 314-747-7230 Email: lett.j.m-at-wustl.edu
Website: http://oto.wustl.edu/Research/Research-Home


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From: microscopy.listserver-at-gmail.com
Date: Sat, 21 Oct 2017 08:26:26 -0500
Subject: [Microscopy] viaWWW: Consulting for HREM architectural workspaces

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Email: amelia.liu-at-monash.edu Name: Amelia Liu

Organization: Monash University

Title-Subject: [Filtered] cryo-FIBSEM position at Monash University

Message: The application closing date for a staff member / Senior Research Officer position to run
our newly acquired cryo-Helios G4 UX FIBSEM and FIBSEM-related projects within the Monash Ramaciotti
Centre for Cryo-Electron Microscopy has been extended to November 12th.

The Ramaciotti Centre houses a suite of microscopes including a Titan Krios (Gatan K2, energy
filter, Volta phase plate, Falcon 3), a Talos Arctica (Volta phase plate, Falcon 3), Tecnai Spririt,
Jeol 1400Plus, and Nova NanoSEM. The centre supports and collaborates on EM projects ranging from
single particle cryo EM, cryo-tomography, immuno EM to correlative light and electron microscopy.

Monash University is located next to the Australian Synchrotron and hosts excellent infrastructure
and expertise in microscopy and imaging through the Monash Centre for Electron Microscopy, the
Monash Ramaciotti Centre for Cryo-EM, Monash Micro Imaging, the Melbourne Centre for
Nanofabrication, Monash Biomedical Imaging and the ARC Centre of Excellence in Advanced Molecular
Imaging.

Senior Research Officer - Cryo-FIBSEM
Remuneration: AU$96,230 - AU$106,221 pa HEW Level 08 (plus 17% employer superannuation)
Closing date: Sunday 12 November 2017, 11.55pm AEDT
Duration: Initial three year fixed-term appointment
Enquiries: georg.ramm-at-monash.edu or +61 3 99051280
Link to the job advertisement and application:
http://careers.pageuppeople.com/513/cw/en/job/566030/senior-research-officer-cryoem


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From isendana0wveyb-at-gmail.com Fri Oct 20 04:16:13 2017
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Email: jpshield-at-uga.edu Name: John Shields

Organization: University of Georgia

Title-Subject: [Filtered] Consulting for HREM architectural workspaces

Message: We are looking for some suggestions of reputable engineering/architectural firms that can
advise and consult with university-contracted architectural firms on design of a new high resolution
EM space that will potentially hold four HREMs and ancillary equipment.

Please respond directly to jpshield-at-uga.edu

Thanks!
John S
UGA Athens

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From: Phil.Ahrenkiel-at-sdsmt.edu
Date: Sun, 22 Oct 2017 09:55:04 -0500
Subject: [Microscopy] Faculty Position-Nanoscience and Nanoengineering

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Assistant or Associate Professor-Nanoscience and Nanoengineering

The South Dakota School of Mines & Technology invites applications for a 9-month tenure-track faculty position in Nanoscience and Nanoengineering at the Assistant or Associate Professor level in the area of computational nanoscience, imaging informatics and bioinformatics. Candidates with expertise in other areas relating to bio-nanoscience will also be considered.

The position is associated with a statewide research center: BioSystems Networks / Translational Research (BioSNTR). BioSNTR is focused on advanced fluorescence and optical imaging methods to leverage bioinformatics towards the understanding of signaling networks and their regulation in living systems. BioSNTR is a collaboration between research university partners South Dakota State University, the University of South Dakota, and South Dakota Mines, with multiple industrial partners, including Sanford Research.

The successful candidate will be provided a competitive salary and access to center staff and shared resources, including high performance computing infrastructure. The selected candidate will be expected to contribute to the South Dakota Mines Nanoscience and Nanoengineering PhD program by developing a robust extramurally funded research program, teaching and developing relevant graduate courses, mentoring and advising PhD students, and building strong collaborative research teams.

Specific interests for this position are candidates who could contribute to multiscale modeling, analyzing high volume microscopy data generated by our newly constructed lattice light sheet microscope, and connecting this data with computational biology and next-generation sequencing. Individuals whose research emphasis can complement on-going efforts in biophotonics, cellular level biophysics, super-resolution imaging methods, mechanobiology, and the combination of AFM and fluorescence microscopy for correlative imaging of bio-systems are particularly encouraged to apply.

Applicants must possess a PhD in a science or engineering discipline closely aligned with Nanoscience and Nanoengineering and/or one of the above-mentioned research emphases. Candidates from underrepresented groups are encouraged to apply. The successful candidate will become a faculty member in an interdisciplinary doctoral program in Nanoscience and Nanoengineering, with an anticipated start date of August 20, 2018. Email Steve.Smith-at-sdsmt.edu for further information regarding this position.

Individuals interested in this position must apply online at http://www.sdsmt.edu/employment. Human Resources can provide accommodation to the online application process and may be reached at (605) 394-1203. Review of applications will begin December 1, 2017, and will continue until the position is filled. Employment is contingent upon completion of a satisfactory background investigation.
------------------------------------------
Phil Ahrenkiel, PhD
Nanoscience and Nanoengineering
South Dakota School of Mines and Technology
501 E. Saint Joseph St.
Rapid City, SD 57701 U.S.A.
Office: EP 221
Phone: 605-394-5238, Cell: 720-988-6627
Phil.Ahrenkiel-at-sdsmt.edu
http://ahrenkiel.sdsmt.edu/



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From: gilpin-at-purdue.edu
Date: Mon, 23 Oct 2017 09:43:11 -0500
Subject: [Microscopy] novel chilled water circulator systems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi everyone,
I have the opportunity to change away from individual chiller units attached to SEMs and TEMs and to work with our physical facilities to design, build and run something new. I am looking at a single system which will cover multiple microscopes. I am aware of the need for strict temperature control, no vibration, cooling and pumping redundancy etc. I wondered if anyone else had gone down this path?

​​​​​
Chris

Christopher J. Gilpin Ph.D.
Director, Life Science Microscopy Facility
Campus-wide Coordinator for Electron Microscopy
Purdue University
Whistler Hall of Agriculture Research, Room S052
170 S. University St
West Lafayette, IN 47907
765-494-7750
gilpin-at-purdue.edu
lsmf-at-purdue.edu reaches everyone in the facility.
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From: John.Mardinly-at-asu.edu
Date: Mon, 23 Oct 2017 10:46:20 -0500
Subject: [Microscopy] Re: novel chilled water circulator systems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Christopher;
When I visited the JEOL factory, I learned that they had a large water tank on the roof to feed all of the microscopes by gravity. The only pumping was to pump the ‘used’ water back up to the tank. Because of the large size of the tank, the water temperature had good stability. There was no turbulence in the water flow, and the overall cost of the system was quite low.

A. John Mardinly, Ph.D., P.E., retired
} On Oct 23, 2017, at 7:56 AM, gilpin-at-purdue.edu wrote:
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} Hi everyone,
} I have the opportunity to change away from individual chiller units attached to SEMs and TEMs and to work with our physical facilities to design, build and run something new. I am looking at a single system which will cover multiple microscopes. I am aware of the need for strict temperature control, no vibration, cooling and pumping redundancy etc. I wondered if anyone else had gone down this path?
}
} ​​​​​
} Chris
}
} Christopher J. Gilpin Ph.D.
} Director, Life Science Microscopy Facility
} Campus-wide Coordinator for Electron Microscopy
} Purdue University
} Whistler Hall of Agriculture Research, Room S052
} 170 S. University St
} West Lafayette, IN 47907
} 765-494-7750
} gilpin-at-purdue.edu
} lsmf-at-purdue.edu reaches everyone in the facility.
} https://urldefense.proofpoint.com/v2/url?u=http-3A__ag.purdue.edu_arp_Microscopy_Pages_default.aspx&d=DwIBaQ&c=l45AxH-kUV29SRQusp9vYR0n1GycN4_2jInuKy6zbqQ&r=VLPJ8OE-c_C6joGeE1ftlvxMmQPq9N6mpKZONBRt90E&m=LgYf1ilTFnw-UUphomi4qg7aBDAK8HkNIwYlTiToZ70&s=13aOqumsN3kWhQfnTCtdnyC16JGs89Hoo4O8P4LTkvs&e=
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From: nicholls-at-uic.edu
Date: Mon, 23 Oct 2017 11:46:15 -0500
Subject: [Microscopy] Re: novel chilled water circulator systems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Chris

We have a building chiller that supplies chilled water for the building
temperature control and for heat exchangers used on the microscopes to
give us the temperature stability we need. Unless it is oversized you
will not get the temperature stability you require from a building
chiller (ours has a 5 degree F range). Ours is outside so needs to be a
30% glycol/water mixture to survive the midwest winters.

This has worked reasonably well for us since 1998, however if (or rather
when) the building chiller goes down all of the microscopes go down as well.

Alan

On 10/23/2017 9:44 AM, gilpin-at-purdue.edu wrote:
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} Hi everyone,
} I have the opportunity to change away from individual chiller units attached to SEMs and TEMs and to work with our physical facilities to design, build and run something new. I am looking at a single system which will cover multiple microscopes. I am aware of the need for strict temperature control, no vibration, cooling and pumping redundancy etc. I wondered if anyone else had gone down this path?
}
} ​​​​​
} Chris
}
} Christopher J. Gilpin Ph.D.
} Director, Life Science Microscopy Facility
} Campus-wide Coordinator for Electron Microscopy
} Purdue University
} Whistler Hall of Agriculture Research, Room S052
} 170 S. University St
} West Lafayette, IN 47907
} 765-494-7750
} gilpin-at-purdue.edu
} lsmf-at-purdue.edu reaches everyone in the facility.
} http://ag.purdue.edu/arp/Microscopy/Pages/default.aspx
}
}
}
}
} ==============================Original Headers==============================
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} 7, 32 -- From: "Gilpin, Christopher J" {gilpin-at-purdue.edu}
} 7, 32 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
} 7, 32 -- Subject: novel chilled water circulator systems
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}

--
Alan W Nicholls, PhD
Associate Director, RRC
Director of Research Service Facility (Electron Microscopy)
Research Resources Center - East (M/C 337)
Room 110, Science and Engineering South Building
The University of Illinois at Chicago
845 West Taylor St
Chicago, IL 60607-7058

Tel:  312 996 1227

Web site http://www.rrc.uic.edu/ems
Wiki site https://wiki.rrc.uic.edu/wiki/EMS


==============================Original Headers==============================
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From: donovan-at-uoregon.edu
Date: Mon, 23 Oct 2017 13:00:12 -0500
Subject: [Microscopy] Re: novel chilled water circulator systems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Alan,

This is exactly the type of system I am trying to set up in our building- do you have any more information you can share on the heat exchanger, and how the regulation of temperature is achieved?

Best regards,

Ben


--
Research Support Manager (Imaging and IT Systems)
MRC Brain Network Dynamics Unit,
University of Oxford Department of Pharmacology,
Mansfield Road, Oxford, OX1 3TH, United Kingdom.
Telephone: 01865 271897 {http://www.mrcbndu.ox.ac.uk/}



-----Original Message-----
X-from: nicholls-at-uic.edu [mailto:nicholls-at-uic.edu]
Sent: 23 October 2017 17:56

Hi Chris,

We have such a "shared" cooling system in our CAMCOR facility that
supports around 10 beam instruments.

http://camcor.uoregon.edu/

We still have each instrument on its own dedicated water cooled chiller
but each chiller is cooled by the shared cooling system. That way we
don't have huge air cooling requirements.  There is a short discussion
of cooling systems here:

http://probesoftware.com/smf/index.php?topic=951.0

john


On 10/23/2017 7:49 AM, gilpin-at-purdue.edu wrote:
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} ----------------------------------------------------------------------------
}
}
} Hi everyone,
} I have the opportunity to change away from individual chiller units attached to SEMs and TEMs and to work with our physical facilities to design, build and run something new. I am looking at a single system which will cover multiple microscopes. I am aware of the need for strict temperature control, no vibration, cooling and pumping redundancy etc. I wondered if anyone else had gone down this path?
}
} ​​​​​
} Chris
}
} Christopher J. Gilpin Ph.D.
} Director, Life Science Microscopy Facility
} Campus-wide Coordinator for Electron Microscopy
} Purdue University
} Whistler Hall of Agriculture Research, Room S052
} 170 S. University St
} West Lafayette, IN 47907
} 765-494-7750
} gilpin-at-purdue.edu
} lsmf-at-purdue.edu reaches everyone in the facility.
} http://ag.purdue.edu/arp/Microscopy/Pages/default.aspx
}
}
}
}
} ==============================Original Headers==============================
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--
John J. Donovan donovan-at-uoregon.edu
University of Oregon (541) 346-4632 (office)
1443 E. 13th Ave (541) 346-4655 (probe)
Eugene, OR (541) 346-6854 (FAX)
97403-1241

CAMCOR Web: http://camcor.uoregon.edu/
Facility Web: http://epmalab.uoregon.edu/
Cameca SX100 Schedule: http://camcor.uoregon.edu/wp/calendar/sx100
Cameca SX50 Schedule: http://camcor.uoregon.edu/wp/calendar/epma
FEI Quanta Schedule: http://camcor.uoregon.edu/wp/calendar/sem


==============================Original Headers==============================
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From: droffle-at-jkraftmicro.com
Date: Mon, 23 Oct 2017 13:00:15 -0500
Subject: [Microscopy] Re: novel chilled water circulator systems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

For those of you interested in a centralized chiller system, our
company can design and deliver an application specific unit that will
provide temperature control and redundancy. Please contact me
directly if you would like to find out more.

On Mon, Oct 23, 2017 at 1:25 PM, {ben.micklem-at-pharm.ox.ac.uk} wrote:
}
}
}
} ----------------------------------------------------------------------------
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} Dear Alan,
}
} This is exactly the type of system I am trying to set up in our building- do you have any more information you can share on the heat exchanger, and how the regulation of temperature is achieved?
}
} Best regards,
}
} Ben
}
}
} --
} Research Support Manager (Imaging and IT Systems)
} MRC Brain Network Dynamics Unit,
} University of Oxford Department of Pharmacology,
} Mansfield Road, Oxford, OX1 3TH, United Kingdom.
} Telephone: 01865 271897 {http://www.mrcbndu.ox.ac.uk/}
}
}
}
} -----Original Message-----
} X-from: nicholls-at-uic.edu [mailto:nicholls-at-uic.edu]
} Sent: 23 October 2017 17:56
} Subject: [Microscopy] Re: novel chilled water circulator systems
}
} Chris
}
} We have a building chiller that supplies chilled water for the building
} temperature control and for heat exchangers used on the microscopes to
} give us the temperature stability we need. Unless it is oversized you
} will not get the temperature stability you require from a building
} chiller (ours has a 5 degree F range). Ours is outside so needs to be a
} 30% glycol/water mixture to survive the midwest winters.
}
} This has worked reasonably well for us since 1998, however if (or rather
} when) the building chiller goes down all of the microscopes go down as well.
}
} Alan
}
}
}
} ==============================Original Headers==============================
} 15, 34 -- From ben.micklem-at-pharm.ox.ac.uk Mon Oct 23 12:02:42 2017
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} 15, 34 -- From: Ben Micklem {ben.micklem-at-pharm.ox.ac.uk}
} 15, 34 -- To: "nicholls-at-uic.edu" {nicholls-at-uic.edu} ,
} 15, 34 -- "microscopy-at-microscopy.com"
} 15, 34 -- {microscopy-at-microscopy.com}
} 15, 34 -- Subject: RE: [Microscopy] Re: novel chilled water circulator systems
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--
Thank You,
Douglas D. Roffle





U.S. 716-592-4402 | FRANCE +330975181013 | FAX 716-592-4407
W jkraftmicro.com | A 243 W Main St | PO Box 386 | Springville, NY 14141

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From: nicholls-at-uic.edu
Date: Mon, 23 Oct 2017 13:53:23 -0500
Subject: [Microscopy] novel chilled water circulator systems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ben

The units were supplied by Haskris (Model WW2 - water to water,
non-refrigerated heat exchanger). They all have electronic close
temperature control which has an immersion heater which is used with a
modulating water control valve. The modulating valve varies the flow of
secondary cooling water to maintain a relatively constant supply water
temperature. The electronically controlled immersion heater switches on
and off to compensate for any remaining fluctuations in the supply water
temperature which results in extremely close temperature control.

Regards

Alan

On 10/23/2017 11:56 AM, Ben Micklem wrote:
} Dear Alan,
}
} This is exactly the type of system I am trying to set up in our building- do you have any more information you can share on the heat exchanger, and how the regulation of temperature is achieved?
}
} Best regards,
}
} Ben
}
}
} --
} Research Support Manager (Imaging and IT Systems)
} MRC Brain Network Dynamics Unit,
} University of Oxford Department of Pharmacology,
} Mansfield Road, Oxford, OX1 3TH, United Kingdom.
} Telephone: 01865 271897 {http://www.mrcbndu.ox.ac.uk/}
}
}
}
} -----Original Message-----
} From: nicholls-at-uic.edu [mailto:nicholls-at-uic.edu]
} Sent: 23 October 2017 17:56
} Subject: [Microscopy] Re: novel chilled water circulator systems
}
} Chris
}
} We have a building chiller that supplies chilled water for the building
} temperature control and for heat exchangers used on the microscopes to
} give us the temperature stability we need. Unless it is oversized you
} will not get the temperature stability you require from a building
} chiller (ours has a 5 degree F range). Ours is outside so needs to be a
} 30% glycol/water mixture to survive the midwest winters.
}
} This has worked reasonably well for us since 1998, however if (or rather
} when) the building chiller goes down all of the microscopes go down as well.
}
} Alan
}

--
Alan W Nicholls, PhD
Associate Director, RRC
Director of Research Service Facility (Electron Microscopy)
Research Resources Center - East (M/C 337)
Room 110, Science and Engineering South Building
The University of Illinois at Chicago
845 West Taylor St
Chicago, IL 60607-7058

Tel:  312 996 1227

Web site http://www.rrc.uic.edu/ems
Wiki site https://wiki.rrc.uic.edu/wiki/EMS


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9, 24 -- From nicholls-at-uic.edu Mon Oct 23 13:53:23 2017
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9, 24 -- Mon, 23 Oct 2017 13:48:34 -0500
9, 24 -- Subject: Re: [Microscopy] Re: novel chilled water circulator systems
9, 24 -- To: Ben Micklem {ben.micklem-at-pharm.ox.ac.uk} ,
9, 24 -- "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
9, 24 -- References: {201710231656.v9NGuNJl008337-at-microscopy.com}
9, 24 -- {168A63C1140FEB4EB61870A513446A4A18A84238-at-MBX03.ad.oak.ox.ac.uk}
9, 24 -- From: Alan Nicholls {nicholls-at-uic.edu}
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From: nicholls-at-uic.edu
Date: Mon, 23 Oct 2017 14:28:06 -0500
Subject: [Microscopy] novel chilled water circulator systems

Contents Retrieved from Microscopy Listserver Archives
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Ben

The units were supplied by Haskris (Model WW2 - water to water,
non-refrigerated heat exchanger). They all have electronic close
temperature control which has an immersion heater which is used with a
modulating water control valve. The modulating valve varies the flow of
secondary cooling water to maintain a relatively constant supply water
temperature. The electronically controlled immersion heater switches on
and off to compensate for any remaining fluctuations in the supply water
temperature which results in extremely close temperature control.

Regards

Alan

On 10/23/2017 11:56 AM, Ben Micklem wrote:
} Dear Alan,
}
} This is exactly the type of system I am trying to set up in our building- do you have any more information you can share on the heat exchanger, and how the regulation of temperature is achieved?
}
} Best regards,
}
} Ben
}
}
} --
} Research Support Manager (Imaging and IT Systems)
} MRC Brain Network Dynamics Unit,
} University of Oxford Department of Pharmacology,
} Mansfield Road, Oxford, OX1 3TH, United Kingdom.
} Telephone: 01865 271897 {http://www.mrcbndu.ox.ac.uk/}
}
}
}
} -----Original Message-----
} From: nicholls-at-uic.edu [mailto:nicholls-at-uic.edu]
} Sent: 23 October 2017 17:56
} Subject: [Microscopy] Re: novel chilled water circulator systems
}
} Chris
}
} We have a building chiller that supplies chilled water for the building
} temperature control and for heat exchangers used on the microscopes to
} give us the temperature stability we need. Unless it is oversized you
} will not get the temperature stability you require from a building
} chiller (ours has a 5 degree F range). Ours is outside so needs to be a
} 30% glycol/water mixture to survive the midwest winters.
}
} This has worked reasonably well for us since 1998, however if (or rather
} when) the building chiller goes down all of the microscopes go down as well.
}
} Alan
}

--
Alan W Nicholls, PhD
Associate Director, RRC
Director of Research Service Facility (Electron Microscopy)
Research Resources Center - East (M/C 337)
Room 110, Science and Engineering South Building
The University of Illinois at Chicago
845 West Taylor St
Chicago, IL 60607-7058

Tel:  312 996 1227

Web site http://www.rrc.uic.edu/ems
Wiki site https://wiki.rrc.uic.edu/wiki/EMS

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9, 24 -- Mon, 23 Oct 2017 13:48:34 -0500
9, 24 -- Subject: Re: [Microscopy] Re: novel chilled water circulator
systems
9, 24 -- To: Ben Micklem {ben.micklem-at-pharm.ox.ac.uk} ,
9, 24 -- "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
9, 24 -- References: {201710231656.v9NGuNJl008337-at-microscopy.com}
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From: microscopy.listserver-at-gmail.com
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Subject: [Microscopy] viaWWW: Job Opportunity - Ted Pella Inc.

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From: microscopy.listserver-at-gmail.com
Date: Tue, 24 Oct 2017 06:17:20 -0500
Subject: [Microscopy] Re: novel chilled water circulator systems

Contents Retrieved from Microscopy Listserver Archives
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X-from: Gary Laevsky {glaevsky.lists-at-gmail.com}

Chris

Don't do this. We tried it at ANL, had a special commerical high capacity unit designed and built to
run all the instruments.

Individual units on each instrument, all of them cooled by a central cooling system is better.


When your SINGLE cooling water system goes down (and it will) then all instruments
are dead in the water. However, if the central chiller which services the small units goes down,
it can still be replaced by a emergency house water line. Since the house water line is simply
taking away the heat from the regulated individual units (all of which have their own controllers)
you can keep all the instruments running.

This also holds true for routine servicing. If you need to change a filter on your water system,
all instruments must be shut down!

It was, in my opinion, our only mistake in SAMMLab and we now essentially use that large single
system to cool individual units, which is huge overkill.

Two other points:

Contamination of the cooling water from all instruments (new and old) gets mixed in the large
single system. If one instrument has a problem and contaminates the water then all instruments
get contaminated.

Not all instruments run best at the "same temperature". We have had to tune the water temperature of
some units to minimize drift and that temperature is a few degree's different than the other
microscopes. This won't likely be an issue with SEM's, but it can be for HREM/AEM's.

Nestor
Your Friendly Neighborhood SysOp



On 10/23/17 10:03 AM CDT, gilpin-at-purdue.edu wrote:
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} Hi everyone,
} I have the opportunity to change away from individual chiller units attached to SEMs and TEMs and to work with our physical facilities to design, build and run something new. I am looking at a single system which will cover multiple microscopes. I am aware of the need for strict temperature control, no vibration, cooling and pumping redundancy etc. I wondered if anyone else had gone down this path?
}
} ​​​​​
} Chris
}
} Christopher J. Gilpin Ph.D.
} Director, Life Science Microscopy Facility
} Campus-wide Coordinator for Electron Microscopy
} Purdue University
} Whistler Hall of Agriculture Research, Room S052
} 170 S. University St
} West Lafayette, IN 47907
} 765-494-7750
} gilpin-at-purdue.edu
} lsmf-at-purdue.edu reaches everyone in the facility.
} http://ag.purdue.edu/arp/Microscopy/Pages/default.aspx
}
}
}
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From: John.Mardinly-at-asu.edu
Date: Tue, 24 Oct 2017 11:08:15 -0500
Subject: [Microscopy] Re: novel chilled water circulator systems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Nestor;
I second this. ASU had a building-wide recirculation system to separate the chillers from the campus chilled water. When it went down, and it did multiple times, every microscope in the building went down. All FEG’s, Real pain.

A. John Mardinly, Ph.D., P.E., Retired, ASU
Now, Classical Guitarist/Lutenist
YouTube Channel:
https://www.youtube.com/user/jmardinly/videos

} On Oct 24, 2017, at 4:30 AM, microscopy.listserver-at-gmail.com wrote:
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} Chris
}
} Don't do this. We tried it at ANL, had a special commerical high capacity unit designed and built to
} run all the instruments.
}
} Individual units on each instrument, all of them cooled by a central cooling system is better.
}
}
} When your SINGLE cooling water system goes down (and it will) then all instruments
} are dead in the water. However, if the central chiller which services the small units goes down,
} it can still be replaced by a emergency house water line. Since the house water line is simply
} taking away the heat from the regulated individual units (all of which have their own controllers)
} you can keep all the instruments running.
}
} This also holds true for routine servicing. If you need to change a filter on your water system,
} all instruments must be shut down!
}
} It was, in my opinion, our only mistake in SAMMLab and we now essentially use that large single
} system to cool individual units, which is huge overkill.
}
} Two other points:
}
} Contamination of the cooling water from all instruments (new and old) gets mixed in the large
} single system. If one instrument has a problem and contaminates the water then all instruments
} get contaminated.
}
} Not all instruments run best at the "same temperature". We have had to tune the water temperature of
} some units to minimize drift and that temperature is a few degree's different than the other
} microscopes. This won't likely be an issue with SEM's, but it can be for HREM/AEM's.
}
} Nestor
} Your Friendly Neighborhood SysOp
}
}
}
} On 10/23/17 10:03 AM CDT, gilpin-at-purdue.edu wrote:
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} }
} } Hi everyone,
} } I have the opportunity to change away from individual chiller units attached to SEMs and TEMs and to work with our physical facilities to design, build and run something new. I am looking at a single system which will cover multiple microscopes. I am aware of the need for strict temperature control, no vibration, cooling and pumping redundancy etc. I wondered if anyone else had gone down this path?
} }
} } ​​​​​
} } Chris
} }
} } Christopher J. Gilpin Ph.D.
} } Director, Life Science Microscopy Facility
} } Campus-wide Coordinator for Electron Microscopy
} } Purdue University
} } Whistler Hall of Agriculture Research, Room S052
} } 170 S. University St
} } West Lafayette, IN 47907
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From: vitalylazar-at-att.net
Date: Tue, 24 Oct 2017 15:12:46 -0500
Subject: [Microscopy] novel chilled water circulator systems

Contents Retrieved from Microscopy Listserver Archives
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Ditto that and very much so.

Unless setup includes instant switching to city water in emergency by
simply turning a few valves. Assuming cooling by city water is suitable
(policy, water quality/temperature, etc.).

Vitaly Feingold
SIA
2773 Heath Lane
Duluth GA 30096
Ph. 770-232-7785
Fax 770-232-1791
www.sia-cam.com
vitaly-at-sia-cam.com

On 10/24/2017 7:19 AM, microscopy.listserver-at-gmail.com wrote:
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} Chris
}
} Don't do this. We tried it at ANL, had a special commerical high capacity unit designed and built to
} run all the instruments.
}
} Individual units on each instrument, all of them cooled by a central cooling system is better.
}
}
} When your SINGLE cooling water system goes down (and it will) then all instruments
} are dead in the water. However, if the central chiller which services the small units goes down,
} it can still be replaced by a emergency house water line. Since the house water line is simply
} taking away the heat from the regulated individual units (all of which have their own controllers)
} you can keep all the instruments running.
}
} This also holds true for routine servicing. If you need to change a filter on your water system,
} all instruments must be shut down!
}
} It was, in my opinion, our only mistake in SAMMLab and we now essentially use that large single
} system to cool individual units, which is huge overkill.
}
} Two other points:
}
} Contamination of the cooling water from all instruments (new and old) gets mixed in the large
} single system. If one instrument has a problem and contaminates the water then all instruments
} get contaminated.
}
} Not all instruments run best at the "same temperature". We have had to tune the water temperature of
} some units to minimize drift and that temperature is a few degree's different than the other
} microscopes. This won't likely be an issue with SEM's, but it can be for HREM/AEM's.
}
} Nestor
} Your Friendly Neighborhood SysOp
}
}
}
} On 10/23/17 10:03 AM CDT, gilpin-at-purdue.edu wrote:
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} }
} } Hi everyone,
} } I have the opportunity to change away from individual chiller units attached to SEMs and TEMs and to work with our physical facilities to design, build and run something new. I am looking at a single system which will cover multiple microscopes. I am aware of the need for strict temperature control, no vibration, cooling and pumping redundancy etc. I wondered if anyone else had gone down this path?
} }
} } ​​​​​
} } Chris
} }
} } Christopher J. Gilpin Ph.D.
} } Director, Life Science Microscopy Facility
} } Campus-wide Coordinator for Electron Microscopy
} } Purdue University
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From: microscopy.listserver-at-gmail.com
Date: Wed, 25 Oct 2017 06:53:53 -0500
Subject: [Microscopy] Fwd: novel chilled water circulator systems

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Name:Lita Drain
School:Howard Hughes Medical Institute


US Email:duraine-at-bcm.edu {mailto:duraine-at-bcm.eduSubject}

Subject {mailto:duraine-at-bcm.eduSubject} :[Microscopy] Re: novel chilled water circulator systems
Your Question:I absolutely agree with Nestor. We have a JEOL 1010 TEM and a JEOL 1400 TEM, both on the same larger chiller. To me it is a pain. When the chiller goes out, both TEMs have to be turned off. We just had a power failure in the one TEM room, but since the one chiller is connected to that room then the chiller went out too. Because of that I had to run to turn off the other TEM in the next separate room. Both instruments were down until I could get power back on. Because of the power failure, the compressor tripped in the chiller and both TEMs were down another day until JEOL could come out and fix it for us. If I had separate chillers, then I would still be in operation with the one TEM. There are many other stories but I won't digress...... Don't do it.....buy separate chillers for each instrument, you will be happier for it.






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From kimgarr459eaus-at-gmail.com Tue Oct 24 17:19:19 2017
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X-from: John Donovan {donovan-at-uoregon.edu}

I agree with Chris. We have the domestic water tied into the process water system so they can be
switched over for maintenance, while as he said, the individual chillers still run each instrument.

Best to have an over temp and flow alarm on the process water system with an alert to your
facilities people so they know when it goes down.

john


On 10/24/2017 4:24 AM, microscopy.listserver-at-gmail.com wrote:
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} Chris
}
} Don't do this. We tried it at ANL, had a special commerical high capacity unit designed and built to
} run all the instruments.
}
} Individual units on each instrument, all of them cooled by a central cooling system is better.
}
}
} When your SINGLE cooling water system goes down (and it will) then all instruments
} are dead in the water. However, if the central chiller which services the small units goes down,
} it can still be replaced by a emergency house water line. Since the house water line is simply
} taking away the heat from the regulated individual units (all of which have their own controllers)
} you can keep all the instruments running.
}
} This also holds true for routine servicing. If you need to change a filter on your water system,
} all instruments must be shut down!
}
} It was, in my opinion, our only mistake in SAMMLab and we now essentially use that large single
} system to cool individual units, which is huge overkill.
}
} Two other points:
}
} Contamination of the cooling water from all instruments (new and old) gets mixed in the large
} single system. If one instrument has a problem and contaminates the water then all instruments
} get contaminated.
}
} Not all instruments run best at the "same temperature". We have had to tune the water temperature of
} some units to minimize drift and that temperature is a few degree's different than the other
} microscopes. This won't likely be an issue with SEM's, but it can be for HREM/AEM's.
}
} Nestor
} Your Friendly Neighborhood SysOp
}
}
}
} On 10/23/17 10:03 AM CDT, gilpin-at-purdue.edu wrote:
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
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} }
} } Hi everyone,
} } I have the opportunity to change away from individual chiller units attached to SEMs and TEMs and to work with our physical facilities to design, build and run something new. I am looking at a single system which will cover multiple microscopes. I am aware of the need for strict temperature control, no vibration, cooling and pumping redundancy etc. I wondered if anyone else had gone down this path?
} }
} } ​​​​​
} } Chris
} }
} } Christopher J. Gilpin Ph.D.
} } Director, Life Science Microscopy Facility
} } Campus-wide Coordinator for Electron Microscopy
} } Purdue University
} } Whistler Hall of Agriculture Research, Room S052
} } 170 S. University St
} } West Lafayette, IN 47907
} } 765-494-7750
} } gilpin-at-purdue.edu
} } lsmf-at-purdue.edu reaches everyone in the facility.
} } http://ag.purdue.edu/arp/Microscopy/Pages/default.aspx
} }
} }
} }
} }
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From: microscopy.listserver-at-gmail.com
Date: Wed, 25 Oct 2017 06:54:48 -0500
Subject: [Microscopy] Fwd: novel chilled water circulator systems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Stephen Kuehn {sckuehn-at-concord.edu}

For water filtration, it helps to install the plumbing and canisters for a redundant pair of
parallel filters with valves on both sides of each filter unit. That way a single filter can be
changed without shutting down the water supply.

If you're really stingy, you can even use this to squeeze a little extra life of a filter which no
longer provides enough flow on its own. Just use one filter alone at first, and when the
flow/pressure begins to drop below acceptable levels, open the valves on the second filter just a
little.

- Steve Kuehn



microscopy.listserver-at-gmail.com wrote:
}
}
} ----------------------------------------------------------------------------
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} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
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} ----------------------------------------------------------------------------
}
} Chris
}
} Don't do this. We tried it at ANL, had a special commerical high capacity unit designed and built to
} run all the instruments.
}
} Individual units on each instrument, all of them cooled by a central cooling system is better.
}
}
} When your SINGLE cooling water system goes down (and it will) then all instruments
} are dead in the water. However, if the central chiller which services the small units goes down,
} it can still be replaced by a emergency house water line. Since the house water line is simply
} taking away the heat from the regulated individual units (all of which have their own controllers)
} you can keep all the instruments running.
}
} This also holds true for routine servicing. If you need to change a filter on your water system,
} all instruments must be shut down!
}
} It was, in my opinion, our only mistake in SAMMLab and we now essentially use that large single
} system to cool individual units, which is huge overkill.
}
} Two other points:
}
} Contamination of the cooling water from all instruments (new and old) gets mixed in the large
} single system. If one instrument has a problem and contaminates the water then all instruments
} get contaminated.
}
} Not all instruments run best at the "same temperature". We have had to tune the water temperature of
} some units to minimize drift and that temperature is a few degree's different than the other
} microscopes. This won't likely be an issue with SEM's, but it can be for HREM/AEM's.
}
} Nestor
} Your Friendly Neighborhood SysOp
}
}
}
} On 10/23/17 10:03 AM CDT, gilpin-at-purdue.edu wrote:
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} }
} } Hi everyone,
} } I have the opportunity to change away from individual chiller units attached to SEMs and TEMs and to work with our physical facilities to design, build and run something new. I am looking at a single system which will cover multiple microscopes. I am aware of the need for strict temperature control, no vibration, cooling and pumping redundancy etc. I wondered if anyone else had gone down this path?
} }
} } ​​​​​
} } Chris
} }
} } Christopher J. Gilpin Ph.D.
} } Director, Life Science Microscopy Facility
} } Campus-wide Coordinator for Electron Microscopy
} } Purdue University
} } Whistler Hall of Agriculture Research, Room S052
} } 170 S. University St
} } West Lafayette, IN 47907
} } 765-494-7750
} } gilpin-at-purdue.edu
} } lsmf-at-purdue.edu reaches everyone in the facility.
} } http://ag.purdue.edu/arp/Microscopy/Pages/default.aspx
} }
} }
} }
} }
} } ==============================Original Headers==============================
} } 7, 32 -- From gilpin-at-purdue.edu Mon Oct 23 09:43:11 2017
} } 7, 32 -- Received: from xppmailspam04.itap.purdue.edu (xppmailspam04.itap.purdue.edu [128.210.5.15])
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} } 7, 32 -- From: "Gilpin, Christopher J" {gilpin-at-purdue.edu}
} } 7, 32 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
} } 7, 32 -- Subject: novel chilled water circulator systems
} } 7, 32 -- Thread-Topic: novel chilled water circulator systems
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} } 7, 32 -- Date: Mon, 23 Oct 2017 14:38:21 +0000
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--

----------------
Dr. Stephen C. Kuehn
Associate Professor
Manager, Electron Microprobe Facility & Tephra Lab
Science building, Room 106

Concord University
1000 Vermillion St
PO Box 1000, Campus Box F20
Athens, WV 24712-1000

sckuehn-at-concord.edu kuehnsc-at-gmail.com
304-384-6322

http://academics.concord.edu/sckuehn/
http://academics.concord.edu/microanalysis/
https://www.researchgate.net/profile/Stephen_Kuehn
ORCID ID orcid.org/0000-0002-2918-980X
ResearcherID A-4946-2016


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From: microscopy.listserver-at-gmail.com
Date: Wed, 25 Oct 2017 06:55:24 -0500
Subject: [Microscopy] Fwd: novel chilled water circulator systems

Contents Retrieved from Microscopy Listserver Archives
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X-from: Soumitra Ghoshroy {rintugr-at-gmail.com}



Chris,

I totally agree. We have individual chillers for each instrument and all chillers are connected to
the building chilled processed water. So it is much easier to service individual chillers without
shutting down all scopes.

Thanks,

Soumitra

/**************************************************/

/Soumitra Ghoshroy/

/Director, Electron Microscopy Center/

/Research Professor, Biological Sciences/

/University of South Carolina/

/Columbia, SC 29208/

/Tel: 803-777-7085/

/Fax: 803-777-8908/

/URL: //http://www.emc.sc.edu/ {http://www.emc.sc.edu/}


On Tue, Oct 24, 2017 at 7:27 AM, {microscopy.listserver-at-gmail.com
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Chris

Don't do this. We tried it at ANL, had a special commerical high capacity unit designed and
built to
run all the instruments.

Individual units on each instrument, all of them cooled by a central cooling system is better.


When your SINGLE cooling water system goes down (and it will) then all instruments
are dead in the water.    However, if the central chiller which services the small units goes down,
it can still be replaced by a emergency house water line. Since the house water line is simply
taking away the heat from the regulated individual units (all of which have their own controllers)
you can keep all  the instruments running.

This also holds true for routine servicing.  If you need to change a filter on your water system,
all instruments must be shut down!

It was, in my opinion, our only  mistake in SAMMLab and we now essentially use that large single
system to cool individual units, which is huge overkill.

Two other points:

Contamination of the cooling water from all instruments (new and old) gets mixed in the large
single system.  If one instrument has a problem and contaminates the water then all instruments
get contaminated.

Not all instruments run best at the "same temperature". We have had to tune the water
temperature of
some units  to minimize drift and that temperature is a few degree's different than the other
microscopes.  This won't likely be an issue with SEM's, but it can be  for HREM/AEM's.

Nestor
Your Friendly Neighborhood SysOp



On 10/23/17 10:03 AM CDT, gilpin-at-purdue.edu {mailto:gilpin-at-purdue.edu} wrote:
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} Hi everyone,
} I have the opportunity to change away from individual chiller units attached to SEMs and TEMs
and to work with our physical facilities to design, build and run something new. I am looking at
a single system which will cover multiple microscopes. I am aware of the need for strict
temperature control, no vibration, cooling and pumping redundancy etc. I wondered if anyone else
had gone down this path?
}
} ​​​​​
} Chris
}
} Christopher J. Gilpin Ph.D.
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From: microscopy.listserver-at-gmail.com
Date: Wed, 25 Oct 2017 06:55:52 -0500
Subject: [Microscopy] Fwd: novel chilled water circulator systems

Contents Retrieved from Microscopy Listserver Archives
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X-from: John Mansfield {jfmjfm-at-umich.edu}


I completely agree with Nestor.
Out current system is single units cooled by a building system.  There is a back up for the building
system I believe.

John Mansfield

Phone: (734) 936-3352

Cell. Phone: (734) 834-3913

Email: jfmjfm-at-umich.edu {mailto:jfmjfm-at-umich.edu}

iMessage: thejfmjfm

Skype: thejfmjfm


On Oct 24, 2017, at 7:29 AM, microscopy.listserver-at-gmail.com
{mailto:microscopy.listserver-at-gmail.com} wrote:

}
}
}
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} Chris
}
} Don't do this. We tried it at ANL, had a special commerical high capacity unit designed and built to
} run all the instruments.
}
} Individual units on each instrument, all of them cooled by a central cooling system is better.
}
}
} When your SINGLE cooling water system goes down (and it will) then all instruments
} are dead in the water.    However, if the central chiller which services the small units goes down,
} it can still be replaced by a emergency house water line. Since the house water line is simply
} taking away the heat from the regulated individual units (all of which have their own controllers)
} you can keep all  the instruments running.
}
} This also holds true for routine servicing.  If you need to change a filter on your water system,
} all instruments must be shut down!
}
} It was, in my opinion, our only  mistake in SAMMLab and we now essentially use that large single
} system to cool individual units, which is huge overkill.
}
} Two other points:
}
} Contamination of the cooling water from all instruments (new and old) gets mixed in the large
} single system.  If one instrument has a problem and contaminates the water then all instruments
} get contaminated.
}
} Not all instruments run best at the "same temperature". We have had to tune the water temperature of
} some units  to minimize drift and that temperature is a few degree's different than the other
} microscopes.  This won't likely be an issue with SEM's, but it can be  for HREM/AEM's.
}
} Nestor
} Your Friendly Neighborhood SysOp
}
}
}
} On 10/23/17 10:03 AM CDT, gilpin-at-purdue.edu {mailto:gilpin-at-purdue.edu} wrote:
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} }
} } Hi everyone,
} } I have the opportunity to change away from individual chiller units attached to SEMs and TEMs and
} } to work with our physical facilities to design, build and run something new. I am looking at a
} } single system which will cover multiple microscopes. I am aware of the need for strict
} } temperature control, no vibration, cooling and pumping redundancy etc. I wondered if anyone else
} } had gone down this path?
} }
} } ​​​​​
} } Chris
} }
} } Christopher J. Gilpin Ph.D.
} } Director, Life Science Microscopy Facility
} } Campus-wide Coordinator for Electron Microscopy
} } Purdue University
} } Whistler Hall of Agriculture Research, Room S052
} } 170 S. University St
} } West Lafayette, IN 47907
} } 765-494-7750
} } gilpin-at-purdue.edu {mailto:gilpin-at-purdue.edu}
} } lsmf-at-purdue.edu {mailto:lsmf-at-purdue.edu} reaches everyone in the facility.
} } http://ag.purdue.edu/arp/Microscopy/Pages/default.aspx
} }
} }
} }
} }
} } ==============================Original Headers==============================
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From: microscopy.listserver-at-gmail.com
Date: Wed, 25 Oct 2017 06:56:33 -0500
Subject: [Microscopy] Fwd: Joint Princeton/Philadelphia Society for Microscopy Symposium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Robert {carltra-at-aol.com}


To all,

Princeton University and the Philadelphia Society for Microscopy will hold a Symposium on Thursday
November 2^nd .  We will have 3 talks in the morning and early afternoon, followed by tours of
Princeton’s microscopy facilities.

You can get more information and register at the following:

http://phillyscope.com/princeton-symposium-2-november-2017/

Thanks

Robert Carlton

President Philadelphia Society for Microscopy

http://phillyscope.com/

https://www.facebook.com/PhiladelphiaSocietyforMicroscopy/?ref=aymt_homepage_panel

carltra-at-aol.com {mailto:carltra-at-aol.com}


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From: john.catino-at-mineralstech.com
Date: Wed, 25 Oct 2017 06:57:07 -0500
Subject: [Microscopy] novel chilled water circulator systems

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We run a main chiller to cool individual chillers. When main chiller goes down, there is an automatic switch to city water to provide cooling.

John

-----Original Message-----
X-from: gilpin-at-purdue.edu [mailto:gilpin-at-purdue.edu]
Sent: Monday, October 23, 2017 10:45 AM
To: John W. Catino {john.catino-at-mineralstech.com}


Hi everyone,
I have the opportunity to change away from individual chiller units attached to SEMs and TEMs and to work with our physical facilities to design, build and run something new. I am looking at a single system which will cover multiple microscopes. I am aware of the need for strict temperature control, no vibration, cooling and pumping redundancy etc. I wondered if anyone else had gone down this path?

​​​​​
Chris

Christopher J. Gilpin Ph.D.
Director, Life Science Microscopy Facility Campus-wide Coordinator for Electron Microscopy Purdue University Whistler Hall of Agriculture Research, Room S052
170 S. University St
West Lafayette, IN 47907
765-494-7750
gilpin-at-purdue.edu
lsmf-at-purdue.edu reaches everyone in the facility.
http://ag.purdue.edu/arp/Microscopy/Pages/default.aspx




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Email: carsten.sachse-at-embl.de Name: Carsten Sachse

Organization: EMBL
Title-Subject: [Filtered] Postdoctoral Fellow – In situ cryo-EM structural cell biology

Message: Dear list members,

In my lab, a position for in situ cryo-EM in the area of structural cell biology is available. I am
looking for a candidate with experience in cryo-ET/EM and/or FIB-SEM for the following project:
http://s.embl.org/HD01191

In case you have further questions, please do not hesitate to contact me. Deadline for the position
is November 3rd.

Best wishes,


Carsten
____________________________________________________________________________________
Dr. Carsten Sachse Group Leader
European Molecular Biology Laboratory Meyerhofstr. 1
69117 Heidelberg
Germany
email: carsten.sachse-at-embl.de
http://www.embl.de/research/units/scb/sachse/
http://www.sachse.embl.de

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From: jkrupp-at-deltacollege.edu
Date: Wed, 25 Oct 2017 16:01:43 -0500
Subject: [Microscopy] Position at Delta College

Contents Retrieved from Microscopy Listserver Archives
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Keep your eyes peeled for the announcement of a teaching position at San Joaquin Delta College.

I retire in Dec. announcement should be out soon.

I can give more info if you are interested.

Jon

Jonathan Krupp
ASB&T
San Joaquin Delta College
5151Pacific Ave.
Stockton, CA 95207
(209) 954-5284
jkrupp-at-deltacollege.edu

After 12/30/17 my email will be:
jkrupp267-at-gmail.com




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From: jkrupp-at-deltacollege.edu
Date: Wed, 25 Oct 2017 16:09:53 -0500
Subject: [Microscopy] Perfect Loop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Looking for practical advice about using the ‘Perfect Loop’ for picking up thin sections.

Does it work for you?

Do you need to clean it before use or between pick ups?

Can it be flamed for cleaning? (They are expensive, so if you have already fried one let me know so I can avoid vaporizing mine)

Anything else worth mentioning?

Thanks

Jon

ps - I will be retiring soon (again), will someone give me permission to recycle all my old papers and journals or suggest an alternative.


Jonathan Krupp
ASB&T
San Joaquin Delta College
5151Pacific Ave.
Stockton, CA 95207
(209) 954-5284
jkrupp-at-deltacollege.edu

After 12/30/17 my email will be:
jkrupp267-at-gmail.com




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From: microscopy.listserver-at-gmail.com
Date: Wed, 25 Oct 2017 21:06:32 -0500
Subject: [Microscopy] Fwd: X-ray interaction with fluorescent dyes

Contents Retrieved from Microscopy Listserver Archives
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X-from: Knecht, David {david.knecht-at-uconn.edu}


A colleague of mine encountered a talk at a recent meeting in which a physician was treating cells
or tissues with acridine orange and then bombarding with X-rays and the AO stained cells were
killed, while the non-treated cells were not. Their target was osteoclasts to try to prevent bone
loss in cancer patients. They seem to have no data on the mechanism, but two hypotheses jump to mind:
1. The AO actually has an absorption/excitation spectrum which includes the wavelength of X-rays
(0.01-10nm) and that absorption causes toxicity (free radicals?)
2. The AO is doing something to the cells to make them more sensitive to X-rays (neutralizing
endosomes and lysosomes) and it has nothing to do with light absorption by the dye.
My question is whether anyone knows if there is data on the behavior of fluorescent dyes like AO in
this very short wavelength light spectrum.

Thanks- Dave

Dr. David Knecht
Professor , Department of Molecular and Cell Biology
University of Connecticut
91 N. Eagleville Rd.
U-3125
Storrs, CT 06269-3125
860-486-2200


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From: PhillipsT-at-missouri.edu
Date: Tue, 31 Oct 2017 10:30:48 -0500
Subject: [Microscopy] microscopy - humor hidden with histology slides

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X-from: Secretary-at-midwestmicroscopy.org

This Question/Comment was submitted to the Microscopy Listserver
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Email: Secretary-at-midwestmicroscopy.org Name: midwestmicroscopy.org

Organization: MMMS

Title-Subject: [Filtered] Winter Meeting of MMMS - Nov, 2017

Message: The winter meeting of the Midwest Microscopy and Microanalysis Society will be held on Nov.
17, 2017 at the Baxter Corporate Headquarters in Deerfield Illinois.

The meeting is Free for M3S members, $20.00 for non-members, $5.00 for students

The theme for this meeting is "Other M: Innovative Microanalysis". Six speakers as listed in the
program outline below will discuss a range of contemporary topics in analysis.

Additional information and directions to Baxter is available at the MMMS WWW site.

http://www.midwestmicroscopy.org

MMMS November 17 Program.

8:00 – 9:00AM Registration, Continental Breakfast will be served

9:00 – 9:10AM Welcome and Opening Remarks

9:10 – 10:00AM Surface Microscopy and Microanalysis in an Industrial Research and Development
Laboratory: General Electric Global Research Center
Vincent Smentkowski, General Electric

10:00 - 10:30AM Mid-IR QCL Spectral Microscopy: Bringing Label-free, Video Rate Chemical Imaging
Modalities to the Materials and Life Sciences
Jeremy Rowlette, Daylight Solutions

10:30 – 11:00AM Break & Visit with Vendors

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11:45AM – 12:10PM MMMS Business Meeting

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1:30PM – 2:15PM SXES Soft X-ray Emission Spectroscopy… Coming Close To Doing the Impossible Vern
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2:15 – 2:45PM Renishaw inVia: the Swiss Army Knife of Confocal Raman Systems
Tim Prusnick, Renishaw

2:45 - 3:15PM Accelerating Materials Characterization with Machine Learning
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From otiscatr45k-at-gmail.com Sat Oct 28 02:39:54 2017
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One of my former histology students sent me this link of "cats hiding in histological specimens". I hope you will forgive me for sharing: http://thebiogeek.com/2016/03/histocats-when-cats-hide-in-histological-specimens/


Thomas E. Phillips, Ph.D
Professor of Biological Sciences
Curator's Distinguished Teaching Professor
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400
573-882-4712 (office)
573-882-0123 (fax)
phillipst-at-missouri.edu

http://www.biology.missouri.edu/faculty/phillips.html
http://microscopy.missouri.edu



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From: microscopy.listserver-at-gmail.com
Date: Tue, 31 Oct 2017 19:25:43 -0500
Subject: [Microscopy] viaWWW: Postdoctoral Position at ORNL on in situ microscopy and data

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Email: unocicrr-at-ornl.gov Name: Raymond Unocic

Organization: Oak Ridge National Laboratory

Title-Subject: [Filtered] Postdoctoral Position at ORNL on in situ microscopy and data analytics

Message: There is a postdoc position open in the microscopy group at the Center for Nanophase
Materials Sciences Division at Oak Ridge National Laboratory.

If interested please go to ornl.gov/careers and apply for the position number NB50644036 "Gas Cell
Microscopy to Probe Interfacial Chemical Reactions"

Brief description is below:

A multidisciplinary research team has been assembled at ORNL to develop and optimize novel electron
microscopy imaging techniques and quantitative data analytic methods to understand catalytic
processes in situ at the atomic and molecular level. As a Postdoctoral Research Associate, you will
design and perform in situ/operando microscopy experiments to determine factors that govern the
atomic-scale restructuring of catalysts during gas reactions. You will work with this team to
develop advanced and correlative electron microscopy imaging and spectroscopy techniques (e.g., 4D
STEM) and quantitative data analytic methods.This post-doc position resides within the Electron and
Atom Probe Microscopy Group at the Center for Nanophase Materials Sciences (CNMS). As a core team
member, you will work with a diverse group of experimentalists, theorists, and data scientists as
part of a project funded through ORNL’s Laboratory Directed Research Development (LDRD) Program.
Your research will emphasize the characterization of structurally and chemically evolving catalyst
nanoparticles using in situ gas cell microscopy, and will be directed toward the correlation of
atomic-scale structural changes with catalytic activity. Your work will focus on the use of
aberration-corrected scanning transmission electron microscopy (STEM) imaging, 4D STEM, ptycography,
and analytical STEM techniques, including electron energy loss spectroscopy (EELS) and energy X-ray
dispersive spectroscopy (EDS). You will also advance microscopy data analytic methods to quantify
time-resolved images for atomic displacements and atomistic restructuring measurements using ORNL’s
high performance computing resources.​

Best Regards

Ray


Raymond R. Unocic, Ph.D.
Oak Ridge National Laboratory
Center for Nanophase Materials Sciences
One Bethel Valley Road
Oak Ridge, TN 37831
unocicrr-at-ornl.gov
865-574-0096

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From: microscopy.listserver-at-gmail.com
Date: Thu, 2 Nov 2017 06:49:58 -0500
Subject: [Microscopy] viaWWW:Postdoctoral fellow positions - cryo-EM of host-pathogen

Contents Retrieved from Microscopy Listserver Archives
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Email: stacie-at-ems-secure.com Name: Stacie Kirsch

Organization: Electron Microscopy Sciences

Title-Subject: [Filtered] 2018 EMS Microscopy Academy New Course Schedule Announced

Message: http://www.emsdiasum.com/microscopy/academy/courses/courses.aspx
We've updated our calendar for 2018! We have some exciting classes at the EMS Microscopy Academy,
including: 1. January 22-26, 2018. Pharmaceutical Microscopy
2. January 30-February 1, 2018 Materials Ultramicrotomy
3. February 13-15, 2018 Automated Rapid Processing
4. February 20-22, 2018 ImmunoGold Silver Staining
5. March 6-8, 2018 Biological SEM
6. March 20-22, 2018 Biological TEM
7. April 3-5, 2018 Material Ultramicrotomy 8. April 10-12, 2018
Cryo SEM
9. April 17-18, 2018 Pharmaceutical Chemical Imaging
10. May 8-10, 2018 Introduction to Microscopy Techniques
11. May 15-17, 2018 X-Ray Microanalysis
12. June 4-8 Cryo Immune and Cryo Sectioning
13. October 8-12, 2018 Cryosectioning and Immunogold
14. October 23-25, 2018 Cryo SEM
15. November 6-8, 2018 Biological TEM
16. November 13-15, 2018 Biological SEM .

Starting Soon – Pharmaceutical Microscopy, featuring expert Robert Carlton, January 22 – 26

Materials Ultramicrotomy, featuring expert Helmut Gnaegi, January 30 – February 1

Automated and Rapid Specimen Processing, featuring expert Michael Kostrna, February 13 - 14

We look forward to seeing you in 2018!

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From tammyhoward072ga-at-gmail.com Thu Nov 2 00:20:11 2017
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Email: erwrigh-at-emory.edu Name: Elizabeth Wright

Organization: Emory University

Title-Subject: [Filtered] Postdoctoral fellow positions - cryo-EM of host-pathogen systems

Message: NIH-funded postdoctoral positions to study either virus assembly or bacterial appendages
and secretion systems are available in the Wright lab (http://electronmicroscopy.emory.edu). Major
research efforts in the lab are focused on 3D structure/function relationships between host cells
and viruses or bacteria. Areas of particular interest include cryo-electron tomography of bacteria,
bacteria – bacteriophage interactions, paramyxoviruses, and HIV-1. Technology development interests
are in the areas of correlative microscopy and phase plate cryo-microscopy.

Representative publications applicable to the projects associated with the position(s): 1. C. M.
Hampton, J. D. Strauss, Z. Ke, R. S. Dillard, J. E. Hammonds, E. Alonas, T. M. Desai, M. Marin, R.
E. Storms, F. Leon, G. B. Melikyan, P. J. Santangelo, P. W. Spearman, and E. R. Wright. “Correlated
Fluorescence Microscopy And Cryo-Electron Tomography Of Virus-Infected And Transfected Mammalian
Cells.” Nature Protocols. 2017: 12 (1): 150-167.
2. C. C. Stobart, C. A. Rostad, Z. Ke, R. S. Dillard, C. M. Hampton, J. D. Strauss, H. Yi, A. L.
Hotard, J. Meng, R. J. Pickles, K. Sakamoto, S. Lee, M. G. Currier, S. M. Moin, B. S. Graham, M. S.
Boukhvalova, B. E. Gilbert, J. C. G. Blanco, P. A. Piedra, E. R. Wright, and M. L. Moore. "A
Live-Attenuated RSV Vaccine With Increased Incorporation Of Pre-Fusion F Exhibits Enhanced Thermal
Stability And Immunogenicity." Nature Communications. 2016: 7: 13916.
3. C. K. Ellison, J. Kan, R. S. Dillard, D. T. Kysela, C. M. Hampton, Z. Ke, E. R. Wright, N. Biais,
A. B. Dalia, and Y. V. Brun. “Obstruction Of Pilus Retraction Stimulates Bacterial Surface Sensing.”
Science. 2017: 358 (6362): 535-538.
4. R. C. Guerrero-Ferreira, P. H. Viollier, B. Ely, J. S. Poindexter, M. Georgieva, G. J. Jensen,
and E. R. Wright. “Alternative Mechanism For Bacteriophage Adsorption To The Motile Bacterium
Caulobacter crescentus.” Proc Natl Acad Sci U.S.A. 2011: 108(24): 9963-9968.
The wet laboratory is within the Division of Pediatric Infectious Diseases and is fully equipped for
all aspects of microbiology, molecular biology, molecular virology, and protein biochemistry. The
Wright lab is equipped with a JEOL JEM-2200FS 200 kV FEG TEM with an in-column energy filter, phase
plate system, Direct Electron DE-20 direct electron detector, and Gatan 4kx4k CCD camera. This
instrument will also serve as a feeder microscope to FSU and other cryo-EM consortia with Titan
Krios cryo-microscopes. For cryo-CLEM development and experiments, there is a Leica cryo-CLEM system
and access to multiple live-cell imaging platforms. The laboratory also has an FEI Vitrobot, a Gatan
CP3 and a manual grid plunge freezer; and a Leica cryo-ultramicrotome. The group has access to all
the resources available in the Emory University EM Core Facility, to include: a JEOL JEM-1400 120 kV
TEM with Gatan 2kx2k CCD camera; a Bal-Tec high-pressure freezer; and Leica freeze substitution system.

Qualifications: Candidates should have a PhD in microbiology, virology, structural biology, or other
related scientific discipline. Candidates should have an interest in structural biology and
microbiology; excellent communication skills; and enjoy working as part of a dynamic and
collaborative research team.
Applicants should include a cover letter describing research experience and interests, curriculum
vitae, and names and contact information for three references. Applications will be reviewed as they
are received and until the position is filled.

Best,

Liz
----
Elizabeth R. Wright, PhD
Associate Professor
Emory University
(404) 727-4665
erwrigh-at-emory.edu

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From: bfoster-at-the-mip.com
Date: Tue, 7 Nov 2017 15:22:08 -0600
Subject: [Microscopy] New book on Nanosafety

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

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Email: Sergio.Sanchez5-at-Honeywell.com Name: Sergio Sanchez

Organization: Honeywell

Title-Subject: [Filtered] JEM-840A Up for grabs

Message: All,
My group is in the process of decommissioning our JEM-840A SEM but would like to see if anyone is in
need of it (parts or the entire instrument) before we will be obligated to dispose of it since it
takes up valuable lab space.

If interested, please contact me:

Sergio I. Sanchez
R&D Manager – Microscopy and Metallurgy
Honeywell UOP
25 E. Algonquin Rd., Des Plaines, IL 60017
Office: +1 847.391.1196
Sergio.Sanchez5-at-Honeywell.com


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From gunnclar77m-at-gmail.com Fri Nov 3 07:30:40 2017
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Email: andy.stewart-at-ul.ie Name: Andrew Stewart

Organization: University of Limerick

Title-Subject: [Filtered] Electron crystallography School

Message: Dear all,

We would like to bring to your attention the deadline (30th of November 2017) for the International
school of Crystallography 2018 on the topic of Electron Crystallography.

The Course intends to review the traditional as well as the modern methods of electron
crystallography; it will be divided into three major fields:
1. provide a strong background on crystallography in general and electron crystallography in particular.
2. introduce students to state-of-art techniques of electron crystallography including experimental
techniques, data acquisition, and data processing as well as to supporting technologies such as
spectroscopy.
3. cover different approaches for structure analysis.

The school will take place in Erice, Italy, at the Ettore Majorana Foundation and center for
scientific culture.
Please apply here: http://crystalerice.org/2018/.

Application Deadline: 30th of November 2017

With kind regards from the course directors, Joke Hadermann, Lukas Palatinus, and Andy Stewart.

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From pedugjuf5b-at-gmail.com Mon Nov 6 18:03:22 2017
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Hi, all

I know that more and more of us are working with
nanomaterials. DeGruyter just published a new textbook (also great
for those of you in industry) for which a colleague of mine was editor:
"Nano-safety: What We Need to Know to Protect Workers" (ISBN-10: 3110373750)

I was honored enough to be asked to write the intro chapter on
Nanotech, Role of the government in Nanosafety, a discussion of the
Market, and Educational programs related to nanosafety. (Caveat: No
commercial interest). The more detailed chapters were written by a
number of pioneers in Nanosafety, including Dr. Walt Trybula and our
own Deb Newbury.

Details are at Amazon.com

Good hunting!
Barbara Foster, President & Chief Consultant
Microscopy/Microscopy Education ... "Education, not JustTraining"
7101 Royal Glen Trail, Suite A - McKinney, TX 75070
P: 972-924-5310 W: www.MicroscopyEducation.com
Microscopy/Microscopy Education is a division of The Microscopy &
Imaging Place, Inc.

NEW! Getting involved in Raman or FTIR?
MME is now offering courses in these areas specifically for
microscopists! Call today for details.




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From: microscopy.listserver-at-gmail.com
Date: Tue, 7 Nov 2017 19:43:30 -0600
Subject: [Microscopy] viaWWW: BSD for Zeiss Supra 40 - again

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Email: gary-at-gaugler.com Name: Gary Gaugler

Organization: Microtechnics

Title-Subject: [Filtered] BSD for Zeiss Supra 40 - again

Message: I'm giving one last attempt to find a decent backscatter detector for Supra 40. This has
been going on for two years with no success.

Zeiss sent a nice Deben Centaurus scintillator unit but it did not fit the chamber--total length was
2cm too short to reach the pole piece. No reasonable option to fix this.

Zeiss won't send a demo QBSD AsB detector to examine without an installation purchase order.
Declined. No pictures nor any instructions to figure out where the thing will go and which port
would be used.

Anyone have any thoughts about how to get a BSD for Supra 40 and retire the KE Type 211 QBSD?
Integration into SmartSEM would be highly desirable but if not available, some fashion of external
input might work.

Gary Gaugler, PhD
Microtechnics


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From: microscopy.listserver-at-gmail.com
Date: Tue, 7 Nov 2017 19:44:14 -0600
Subject: [Microscopy] viaWWW:Seeking Materials Science Product Specialist

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Title-Subject: [Filtered] Seeking Materials Science Product Specialist

Message: Live and work in one of the most beautiful areas in California – Redding, CA. Ted Pella
Inc. is seeking a Materials Science Product Specialist, who will be responsible product development
and sales/marketing activities related to our SEM, Materials Science, Forensic and AFM product
lines. Exciting times for this position as we recently acquired the assets of a product lines for
semi-conductor industry. Must have BS in Materials Science, 5 years hands on SEM instrument use and
specimen prep experience, and 2 years TEM instrument use and specimen prep experience. To apply
email letter of interest, resume and salary requirements. For complete job posting see our website
www.tedpella.com or LinkedIn.


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From: microscopy.listserver-at-gmail.com
Date: Wed, 8 Nov 2017 22:03:07 -0600
Subject: [Microscopy] viaWWW:Suboptimal HPF cooling rates and reparation?

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Email: kuryu-at-rockefeller.edu Name: Kunihiro Uryu

Organization: The Rockefeller University

Title-Subject: [Filtered] Subptimal HPF cooling rates and reparation?

Message: Dear List,

We have been experiencing suboptimal cooling rates of High Pressure Freezer (HFP). It used to be
easily 20,000 K degree/sec or higher. Now it is ~18,000 K degree/sec at the best. I couldn't find a
written specification of the cooling rates. Done anyone have it? Our experience indicates that
quality of data become questionable, if it gets 17,000 K degree/sec or lower. Has anyone experienced
something similar? Has anyone been able to repair HPF from the condition like this and get 20,000 K
degree/sec or higher again? If so, can you share how you did it?
Many thanks,

Regards,
Hiro

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From: microscopy.listserver-at-gmail.com
Date: Wed, 8 Nov 2017 22:04:33 -0600
Subject: [Microscopy] viaWWW:Gatan Solarus Plasma Cleaner -hydrogen passivation

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Email: lavoie-at-uw.edu Name: Ellen Lavoie

Organization: Univ Washington

Title-Subject: [Filtered] Gatan Solarus Plasma Cleaner
Message: Although I think I already know the answer to this question thought I'd get some more
thoughts. I've been trying to get hold of someone at Gatan with no luck so to you all I ask...I
have a student who would like to do a "hydrogen passivation" experiment which is basically
bombarding a diamond sample with H2 only in a vacuum. I'm trying to determine if the Solarus can be
used for H2 gas only (typically a mixture only is used) and if any longer than a couple of minutes
might be a problem. Thoughts?? My, and a chemistry colleague are guessing, no, it will be a
problem. Please send me a personal email.
Cheers,
Ellen
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From: microscopy.listserver-at-gmail.com
Date: Thu, 9 Nov 2017 07:17:06 -0600
Subject: [Microscopy] viaWWW:Post Doctoral Researcher in Electron Microscopy - University

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Email: Ursel.Bangert-at-ul.ie Name: Ursel Bangert

Organization: Bernal Institute, University of Limerick, Ireland

Title-Subject: [Filtered] Post Doctoral Researcher in Electron Microscopy

Message: A position for a Post Doctoral Researcher in Electron Microscopy is available in the Bernal
Institute at the University of Limerick (UL), Ireland, as part of a US-Ireland partnership project
in collaboration with groups at the University of Nebraska, USA and Queen’s University Belfast,
Northern Ireland. The overall aim of the project is to develop routes for engineering and control of
ferroelectric domain walls and exploring their structure and electronic properties with the
intention to exploit them in advanced nanoelectronic devices. UL’s role is to carry out
high-resolution electron microscopy and spectroscopy studies. An FEI Titan Themis TEM (double
corrected, monochromated, with analytical, i.e., Super-EDX and Quantum EELS capacities, and equipped
with in-situ holders and a Gatan K2 detector) will be used to assess and reveal nano- and
electronic-structure of ferroelectric materials grown in this project (including in-situ studies).
For further details of the position please follow the link http://www.ul.ie/hrvacancies/; the job ID
is 024648.


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From: microscopy.listserver-at-gmail.com
Date: Thu, 9 Nov 2017 08:51:25 -0600
Subject: [Microscopy] viaWWW:SubOptimal HPF cooling rates and reparation-CORRECTION

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Email: kuryu-at-rockefeller.edu Name: Kunihiro Uryu

Organization: The Rockefeller University

Title-Subject: [Filtered] Subptimal HPF cooling rates and reparation

Message: Dear List,

Apology for typo in the earlier communication.

We have been experiencing suboptimal cooling rates of High Pressure Freezer (HFP). It used to be
easily 20,000 K degree/sec or higher. Now it is ~18,000 K degree/sec at the best. I have been
searching for a written specification of the cooling rate, but to no avail. I wonder anyone has it.
Our experience indicates that quality of data became questionable if it got 17,000 K degree/sec or
lower. Has anyone experienced something similar?
Has anyone been able to repair HPF from the condition like this to 20,000 K degree/sec or higher
again? If so, would you mind sharing how you did it?

Many thanks,

Regards,
Hiro Uryu


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From: CGorman-at-hookecollege.com
Date: Fri, 10 Nov 2017 12:10:58 -0600
Subject: [Microscopy] Scanning Electron Microscopy Short Course

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings,

Hooke College of Applied Sciences, located in Westmont, IL, is offering a Scanning Electron Microscopy short course December 10-15, 2017. In addition to lectures, this course emphasizes hands-on training using state-of-the-art equipment.

For further SEM training details and registration information, please follow the link below:

https://www.mccrone.com/scanning-electron-microscopy-course

Best regards-
__________________________________________________
Chris



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From: microscopy.listserver-at-gmail.com
Date: Sat, 11 Nov 2017 18:53:47 -0600
Subject: [Microscopy] viaWWW:Job Opening / Electron Microscopy Contractor NIH

Contents Retrieved from Microscopy Listserver Archives
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Dear List Members,

We are advertising for 2-year postdoctoral position in LA-ICPMS at University of Tasmania. The advertisement is below. If you know of anyone who may be interested, please pass it on. Please contact Leonid for any questions, see his contact details in the ad below.

Many thanks,
Karsten


The position is a fixed-term 2-year appointment funded jointly by Laurin Technic and CODES Analytical Laboratories. The position will involve conducting research aimed at developing new LA-ICPMS techniques and understanding the fundamentals of laser ablation, assisting with operating one LA-ICPMS unit within CODES Analytical Laboratories (a quadrupole ICPMS and an excimer laser microprobe) and assisting with performing analyses for external users.
To be considered, you will have:
PhD, or equivalent, in geochemistry.
Demonstrated ability to conduct research and publish research outcomes in scholarly peer-review journals
Demonstrated experience working with LA-ICPMS instrumentation.
Demonstrated ability to write reports and deliver scientific presentations
Demonstrated high-level written and oral communication skills.
Appointment to this role will be at Academic Level A and will have a total remuneration package of up to $102,848 comprising base salary within the range of $65,798 to $87,905 plus 17% superannuation.
For further information about this position please contact Professor Leonid Danyushevsky, Head of Earth Science, L.Dan-at-utas.edu.au / 03 6226 2469.
To apply, please visit https://jobs.utas.edu.au/psp/ps/EMPLOYEE/HRMS/c/HRS_HRAM.HRS_APP_SCHJOB.GBL?FOCUS=Applicant&SiteId=1 and search for position No. 1476. Your application must as a minimum include your resume, a cover letter and your responses to the position selection criteria. The position description, including the selection criteria, is available on the above website.

Dr Karsten Goemann
Senior Research Fellow, Scanning Electron Microscopy & X-Ray Microanalysis
Central Science Laboratory, University of Tasmania
Mail: Private Bag 74, Hobart TAS 7001, Australia
Deliveries: Chemistry Building Loading Bay, Dobson Road, Sandy Bay TAS 7005, Australia
Location: Rooms 254-256, Chemistry Building, Dobson Road, Sandy Bay TAS 7005, Australia
T: +61 (0)3 6226 2146 | F: +61 (0)3 6226 2494 | M: +61 (0)407 101 990
www.utas.edu.au/research/central-science-laboratory
CRICOS 00586B



University of Tasmania Electronic Communications Policy (December, 2014).
This email is confidential, and is for the intended recipient only. Access, disclosure, copying, distribution, or reliance on any of it by anyone outside the intended recipient organisation is prohibited and may be a criminal offence. Please delete if obtained in error and email confirmation to the sender. The views expressed in this email are not necessarily the views of the University of Tasmania, unless clearly intended otherwise.



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Email: christopher.bleck-at-nih.gov Name: Christopher Bleck

Organization: National Institutes of Health, NHLBI Electron Microscopy
Core Facility
Title-Subject: [Filtered] Job Opening / Electron Microscopy Contractor

Message: This position is within the National Heart, Lung, and Blood
Institute at The National Institutes of Health (NIH), a part of the U.S.
Department of Health and Human Services.

The Electron Microscopy core places a strong emphasis on creating an
environment where scientists and physician-scientists can work together
on disease-specific issues using the most appropriate approaches
available. There are great interactions with a wide range of independent
research groups, and the position offers exceptional opportunities for
interdisciplinary collaboration within and outside of the NIH.

If interested, see the full job description and apply at the following
location:
https://www.idealist.org/en/government-job/1277d9e4dee1437d9a52c7cc3511d09d-electron-microscopy-contractor-national-heart-lung-and-blood-institute-bethesda?

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From: microscopy.listserver-at-gmail.com
Date: Sat, 11 Nov 2017 18:54:34 -0600
Subject: [Microscopy] viaWWW:European EELS & EFTEM School at Graz University

Contents Retrieved from Microscopy Listserver Archives
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Email: jhyun-at-gatan.com Name: John Hyun

Organization: Gatan

Title-Subject: [Filtered] European EELS & EFTEM School at Graz University

Message: European EELS & EFTEM School at Graz University

Graz University of Technology, Graz, Austria
6 – 9 February 2018

This four-day, hands-on laboratory school will take you step-by-step
through the acquisition and analysis of EFTEM and STEM-EELS data. The
school will utilize the state-of-the-art facilities and faculty of EELS
experts at FELMI-ZFE. The featured microscope system that will be used
is a monochromated probe-corrected (S)TEM with a DualEELS system.

EELS experts will train you in the latest EELS and EFTEM instruments and
software and present and demonstrate fundamental principles and methods
essential to acquire optimal EELS spectra, STEM-EELS spectrum images,
energy-filtered images, and elemental maps.

The ultimate goal is to provide you with the training and knowledge
needed to acquire your best EELS and EFTEM results.

Seating is very limited. We encourage you to register now. If you have
any questions, please contact Dr. Gerald Kothleitner, Graz University:
gerald.kothleitner-at-felmi-zfe.at.

Registration:
https://www.felmi-zfe.at/teaching/lll-courses/european-eels-eftem-school/


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From: microscopy.listserver-at-gmail.com
Date: Sat, 11 Nov 2017 18:55:55 -0600
Subject: [Microscopy] viaWWW: Gatan PanaCL Filter Housing

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Email: wfschneider-at-wisc.edu Name: Bil Schneider

Organization: UW Madison Geosciences

Title-Subject: [Filtered] Gatan PanaCL Filter Housing

Message: I'm searching for a spare filter housing assembly for our Gatan
PanaCL detector for the SEM. I've called Gatan a few times in an attempt
to procure the part but they never get back to me. If you have a new or
used filter housing assembly for the PanaCl detector or know where I
might find one please contact me. I cannot find a part number, but it is
the housing that has four one inch openings for standard circular
optical filters.

Thank You,
Bil Schneider SEM Manager UW Geosciences
University of Wisconsin, Madison
wfschneider-at-wisc.edu

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From: microscopy.listserver-at-gmail.com
Date: Sun, 12 Nov 2017 13:39:31 -0600
Subject: [Microscopy] viaWWW:position at the Electron Microscopy Science Technology

Contents Retrieved from Microscopy Listserver Archives
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X-from: raffaella.carzaniga-at-crick.ac.uk

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Email: raffaella.carzaniga-at-crick.ac.uk Name: raffaella carzaniga

Organization: The Francis Crick Institute London (UK)

Title-Subject: [Filtered] few days left to apply for a position at the
Electron Microscopy Science Technology platform - The Francis Crick
Institute - London (UK)

Message: Dear colleagues,

Last few days to apply to be part of the EM stp team at The Francis
Crick Institute in London.
Role title: Senior Laboratory Research Scientist (Electron
Microscopy) Location: The Francis Crick Institute, Midland Road, London
Contract: Fixed-term (4 years), Full time
Salary: Competitive with benefits, subject to skills and experience
Vacancy ID: 6204
The Role To take on this position, you will have a degree and PhD in
the biological sciences, expertise in electron microscopy (EM) and the
application of EM to biomedical research questions, and enthusiasm for
working in a facility environment. Expertise in standard resin-embedding
of cells and tissues, ultramicrotomy, scanning and transmission EM are
essential. Expertise in advanced sample preparation techniques (such as
cryo-preparation and correlative workflows), advanced electron
microscopes (Serial Block Face SEM, Focused Ion Beam SEM, cryo-EM),
other imaging modalities (light and X-ray microscopy), and image
analysis are desirable. This post offers an unparalleled opportunity to
apply your microscopy skills to exciting and innovative research across
biomedical disciplines, with access to cutting edge instrumentation, and
the chance to develop completely new imaging techniques and technologies
for direct application to biomedical research. The role reports to the
Deputy Head of the EM STP.
If you are interested in applying for this role, please apply via our
website https://www.jobs.crick.ac.uk
The closing date for applications is 16 November 2017 at 23:30 pm.

------------------------------------------------------------

Best wishes
Raffa
Raffaella Carzaniga PhD
Deputy Head of Electron Microscopy
The Francis Crick Institute
1 Midland Road
London
NW1 1AT
UK
T: +44 (0) 20379 61732
E: Raffaella.carzaniga-at-crick.ac.uk
W: www.crick.ac.uk






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From: microscopy.listserver-at-gmail.com
Date: Mon, 13 Nov 2017 05:48:24 -0600
Subject: [Microscopy] viaWWW:Electron Microscopist Position Available-Rockefeller

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Email: kuryu-at-rockefeller.edu Name: Kunihiro Uryu

Organization: The Rockefeller University

Title-Subject: [Filtered] Electron Microscopist Position Available

Message: Dear List,

The Rockefeller University, a premier biomedical research institution,
seeks a Research Support-at-Associate or Specialist to join our Electron
Microscopy Resource Center (EMRC).

The EMRC provides state-of-the-art electron microscopy support for
analysis of a wide variety of biological samples, including viruses,
bacteria, insects, animal tissue as well as cultured cells and isolated
cellular components for structural analyses or immuno-electron
microscopy. The EMRC is equipped with three transmission electron
microscopes, a conventional and a serial block-face imaging scanning
electron microscope, and a high-pressure freezing and a
freeze-substitution unit. (http://www.rockefeller.edu/emrc/)

The Research Support Associate/Specialist will participate in all of the
EMRCfs daily operations, including maintenance, upkeep and use of the
electron microscopes and associated equipment, ordering supplies,
interacting with vendors, and administrative support for office duties,
including center billing. The position also entails specimen
preparation, including negative staining, ultrathin sectioning, and
immunolabeling, operation of the microscopes and associated equipment,
training users, as well as consulting scientists on the design of
experiments, data processing/analysis, interpretation of results, and
informing users on the latest methodology through familiarity with
relevant literature.

The successful candidate will have an M.S./Ph.D. degree or equivalent
background in biology, bioengineering or a related field and must have a
minimum of 5 years of hands-on experience in electron microscopy. A
strong background in computation would be a plus. Must have strong
communication skills, and the ability to work collaboratively in a team
as well as independently on a wide variety of research projects. Must be
detail-oriented, focused, and highly motivated.

We offer a competitive salary, comprehensive benefits, and a collegial
work environment. To apply to this job, click the following URL, click
on 'staff opportunitiesf and enter keyword eIRC20449f or 'Electron
Microscopist': http://www.rockefeller.edu/hr/career.php

The Rockefeller University is an Affirmative Action/Equal Employment
Opportunity/VEVRAA employer.

Kunihiro Uryu, Ph.D.
Director, Electron Microscopy Resource Center





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From: eikonika-at-otenet.gr
Date: Fri, 17 Nov 2017 04:39:54 -0600
Subject: [Microscopy] JSM5600LV burns filaments immediately

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Two positions are available in the electron microscopy facility at the MRC Laboratory of Molecular Biology in Cambridge

One position is for an EM engineer, the other for a senior research support assistant. The two positions are part of the team that manage, maintains and develops the facility, supports users, and gets involved in collaborative projects aimed at improving cryo-EM.

The LMB has a vibrant cryo-electron microscopy community. The facility will soon have three Titan Krios, two Polara, two other FEG microscopes, a Scios dual-beam and lower-end microscopes. It has approximately 150 active users applying electron cryomicroscopy and cryo-electron tomography to study important biological problems. There are research programmes aimed at the development of new hardware and software for cryo-electron microscopy.

Further details are available here:

EM engineer position: https://mrc.tal.net/vx/mobile-0/appcentre-ext/brand-3/candidate/so/pm/4/pl/1/opp/702-Electron-Microscopy-EM-Engineer-Structural-Studies-EM-Facility-LMB-702/en-GB

EM research support assistant position: https://mrc.tal.net/vx/mobile-0/appcentre-ext/brand-3/candidate/so/pm/4/pl/1/opp/691-Research-Support-Officer-Structural-Studies-Electron-Microscopy-Facility-LMB-691/en-GB

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Email: marcello.serracino-at-uniroma1.it Name: Marcello Serracino

Organization: Sapienza University Rome Italy

Title-Subject: [Filtered] FEI Quanta 400 problem

Message: Dear all,
we are in trouble with our SEM FEI Quanta 400,
It is not possible to have HV because till the messages listed below
appear the hv is halted.
we have the following messages:
- The gun is in preoperation state and after few seconds
- The internal gun communication has been restored.

Every two minutes we have the same alert.

We remove the Wehnelt cylinder, close the gun, repumping, but the
problem remain.
The vacuum it is ok.
Have you any suggestions?
Every suggestion will be appreciated

Best regards Marcello



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Institute of Materials Science, University of Connecticut 
Position ID: UConn-IMS-2018203 [#10300, 2018203]
Position Title:  Assistant/Associate/Full Professor, Electron Microscopy
Position Type: Tenured/Tenure-track faculty
Position Location: Storrs, Connecticut 06269, United States
Subject Area: Materials Science / Engineering
Appl Deadline: none (posted 2017/10/25)
To apply: https://academicjobsonline.org/ajo/jobs/10300

Position Description:

The Institute of Materials Science (IMS) http://www.ims.uconn.edu/ at the University of Connecticut (UConn) seeks qualified candidates for an Electron Microscopy faculty position (Assistant/Associate/Full Professor) experienced with the advanced electron microscopy methods. The IMS consists of faculty members in several different departments of the College of Liberal Arts and Sciences and the School of Engineering.  The Department appointment will be based on the expertise of the successful candidate.
UConn is in the midst of a transformational period of growth, supported by the $1.7B Next Generation Connecticut (http://nextgenct.uconn.edu/ ), the Tech Park initiative (http://innovation.uconn.edu/tech-park/ ) and a bold new Academic Plan (http://issuu.com/uconnprovost/docs/academic-plan-single-hi-optimized_1 ). We are pleased to continue these investments by inviting applications from eminent scholars who can engage with our IMS faculty members.

DUTIES AND RESPONSIBILITES
The successful candidate will be expected to contribute to research and scholarship through extramural funding (in disciplines where applicable), high quality publications, impact as measured through citations, performances, and exhibits (in disciplines where applicable), and national recognition as through honorific awards. In the area of teaching, the successful candidate will share a deep commitment to effective instruction at the undergraduate and graduate levels, development of innovative courses and mentoring of students in research, outreach, and professional development. Successful candidates will also be expected to broaden participation among members of under-represented groups; demonstrate through their research, teaching, and/or public engagement the richness of diversity in the learning experience; integrate multicultural experiences into instructional methods and research tools; and provide leadership in developing pedagogical techniques designed to meet the needs of diverse learning styles and intellectual interests. 
Successful candidates will teach courses at both the undergraduate and graduate levels, develop internationally recognized, externally-funded research programs, and contribute to the operation and promotion of the Institute, University, and profession through service.

MINIMUM QUALIFICATIONS
1 Applicants must have a Ph.D. in materials science, engineering, physics, chemistry or a related field.
2 Outstanding record of peer-reviewed publications.
3 A history of strong extramurally funded research programs.
4 Experience with teaching courses at the undergraduate and graduate levels.
5 Excellent oral and written communication skills.

PREFERRED QUALIFICATIONS
1 Demonstrated ability to obtain sustained extramural support for research programs.
2 Demonstrated ability to conduct and lead collaborative interdisciplinary research in materials science.
3 Demonstrated ability to work within a research cluster.
4 Excellence in teaching at the undergraduate and graduate levels.
5 Proven commitment to working within a diverse environment.

APPOINTMENT TERMS
This is a full-time (9-month) tenure track position within the IMS. The successful candidate’s primary academic appointment will be at the UConn main campus in Storrs, CT, with the possibility of work at UConn’s regional campuses across the state. Salary and rank will be commensurate with qualifications and experience.

TO APPLY
Select "Apply Now" to be redirected to Academic Jobs Online to complete your application. Please submit a cover letter; curriculum vitae, including a full list of publications; teaching statement (teaching philosophy, teaching experience, commitment to effective learning, concepts for new course development, etc.); research and scholarship statement (innovative concepts, experience in proposal development, mentorship of post-graduate residents, fellows, and/or graduate students, etc.); and a commitment to diversity statement (including broadening participation, integrating multicultural experiences in instruction and research and pedagogical techniques to meet the needs of diverse learning styles, etc.).  Additionally, please follow the instructions on Academic Jobs Online to direct three (3) reference writers to submit letters of reference referencing Electron Microscopy Search # 2018203.
Evaluation of applicants will begin immediately and continue until the position is filled. Employment of the successful candidate will be contingent upon the successful completion of a pre-employment criminal background check. (Search # 2018203)
All employees are subject to adherence to the State Code of Ethics, which may be found at http://www.ct.gov/ethics/site/default.asp.

The University of Connecticut is committed to building and supporting a multicultural and diverse community of students, faculty, and staff. The diversity of students, faculty, and staff continues to increase, as does the number of honors students, valedictorians and salutatorians who consistently make UConn their top choice. More than 100 research centers and institutes serve the University’s teaching, research, diversity, and outreach missions, leading to UConn’s ranking as one of the nation’s top research universities. UConn’s faculty and staff are the critical link to fostering and expanding our vibrant, multicultural and diverse University community. As an Affirmative Action/Equal Employment Opportunity employer, UConn encourages applications from women, veterans, people with disabilities and members of traditionally underrepresented populations.



Application Materials Required:
Submit the following items online at this website to complete your application:
• Cover Letter
• Curriculum Vitae
• Teaching Statement
• Research and Scholarship Statement
• Commitment to Diversity Statement
• Three Reference Letters (to be submitted by the reference writers at https://academicjobsonline.org/ajo/jobs/10300)
And anything else requested in the position description.

Further Info:
http://www.ims.uconn.edu/
 
Institute of Materials Science (IMS)
97 North Eagleville Road
University of Connecticut
Storrs, CT 06269-3136




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From ralpzand8seine-at-gmail.com Thu Nov 16 17:45:32 2017
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Dear List
Our SEM JSM5600LV burns filaments immediately upon turning high tension ON.
There was a thunderstorm here and after a big thunder the microscope went
completely OFF while there was no power cut. Very soon the microscopes
power came back and worked normally but I noticed the image was bit noisy
and definition less high. Then I switched it off overnight and today I
started and realized the problem (after I burned a couple of filaments). Any
comment or suggestion will be greatly appreciated

yorgos

Dr Yorgos Nikas
Athens Innovative Microscopy
Skra 36 Voula 16673 GREECE

Tel/fax +30 210 8957677
mobile +30 6945 107477
www.eikonika.netwww.aim.cat
*************************************




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6, 20 -- From eikonika-at-otenet.gr Fri Nov 17 04:39:54 2017
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6, 20 -- Subject: JSM5600LV burns filaments immediately
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From: sergei2-at-ornl.gov
Date: Fri, 17 Nov 2017 09:02:34 -0600
Subject: [Microscopy] Postdoctoral position: Atom-by-atom fabrication by e-beam in STEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues - we are opening postdoctoral position(s) in the field
of direct atom-by-atom fabrication by sub-atomically focused beam of
Scanning Transmission Electron Microscope. The recent examples of the
work the candidate will be involved in are available in recent
publications (arXiv:1711.05810 {https://arxiv.org/abs/1711.05810} ,
arXiv:1710.10338 {https://arxiv.org/abs/1710.10338} , arXiv:1710.09416
{https://arxiv.org/abs/1710.09416} , arXiv:1708.01523
{https://arxiv.org/abs/1708.01523} , arXiv:1709.00470
{https://arxiv.org/abs/1709.00470} ). The ideal candidate will have
expertise in atomically resolved STEM, image simulation, and Python
programming. Please contact Sergei Kalinin and Stephen Jesse directly
(sergei2-at-ornl.gov and sjz-at-ornl.gov) if interested.
Sergei

--
Sergei V. Kalinin

Director,
Institute for Functional Imaging of Materials

Fellow MRS, APS, AVS

Oak Ridge National Laboratory

Phone: (865) 241-0236


==============================Original Headers==============================
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From: microscopy.listserver-at-gmail.com
Date: Fri, 17 Nov 2017 17:18:04 -0600
Subject: [Microscopy] viaWWW:SEM-CL missing red wavelengths?

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X-from: fay1-at-email.arizona.edu

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Email: fay1-at-email.arizona.edu Name: Isabel Barton

Organization: University of Arizona

Title-Subject: [Filtered] SEM-CL missing red wavelengths?

Message: Dear Microscopy Community:

Does anybody know why an SEM-CL instrument could be missing the
red-to-orange wavelengths of the cathodoluminescence spectrum?

I run a JEOL 6010LA benchtop SEM wit a recently acquired Gatan MonoCL4
cathodoluminescence spectrometer. It works great for zircons, quartz,
etc., but picks up little to no signal in the red to orange part of the
spectrum even on samples that glow brilliant red under our ancient
optical cold-cathode Luminoscope. I checked the same samples out on a
ChromaCL hooked up to a Hitachi S3400 in another lab, and there is a
similar lack of red signal.
I've checked and the accelerating voltages on the Luminoscope are about
the same as on the SEM, although the Luminoscope has higher beam
current, so I'm not sure why the SEM-CL instruments don't pick up the
red luminescence. I’ve read that filters placed in front of a detector
can eliminate red-orange luminescence and phosphorescence (Reed and
Milliken, Journal of Sedimentary Research 73.2: 328-332). There was no
mention of such a filter in the Gatan datasheets for either the ChromaCL
or the MonoCL4, but that seems to me to be one possible explanation for
the lack of red luminescence in SEM-CL compared to optical cold-cathode
CL. I've left a message with Gatan but haven't heard back yet.
Has anybody else with SEM-CL encountered a similar problem, or know of
any fixes? We got the MonoCL4 partly in order to look at carbonate
cements in rocks, so it’s pretty important that we figure out how to get
good spectra and images in the red and orange zones.

Any help would be appreciated.
Thanks,

Isabel Barton

Login Host: 68.106.21.127
Listserver Email Form V - 20120416
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From: microscopy.listserver-at-gmail.com
Date: Fri, 17 Nov 2017 17:18:56 -0600
Subject: [Microscopy] viaWWW:EDS and SEM projector for Outreach

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Email: annie.muske-dukes-driggs-at-lonza.com Name: Annie Muske-Dukes

Organization: Bend Research

Title-Subject: [Filtered] EDS and SEM projector for Outreach

Message: Hi everyone,

My company runs a community outreach program for local elementary school
students (8-10 years old). They tour our facility and see quick
demonstrations/lectures on a variety of topics.
We have had an SEM demo for years and I would like to incorporate our
EDS but I'm having trouble coming up with samples that kids that age
understand, especially since I only have 15 minutes per group and we try
to go through at least four samples.

I have generated a map of different elements in a vitamin tablet which
went over well and gives them a good understanding of what the system is
doing. Any recommendations for samples that generate interesting maps or
have unexpected elements? I'll be doing the prep/high quality maps ahead
of time so that doesn't need to be done in the 15 minutes.

I'd also like to get a wireless projector to use with our Hitachi SU3500
to project a live image of the screen onto the wall. Our service rep
said some models can cause driver issues so if anyone here has a model
that works please let me know. We need to stay under US$500, but under
$300 would be ideal.

Thanks,
Annie (I am a list subscriber but our required corporate attachments
prevent me from sending the usual way)

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From: microscopy.listserver-at-gmail.com
Date: Fri, 17 Nov 2017 17:19:29 -0600
Subject: [Microscopy] viaWWW:Electron Microscopy Position Available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: walbrid-at-cshl.edu
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Email: walbrid-at-cshl.edu Name: Samantha

Organization: Cold Spring Harbor Laboratory

Title-Subject: [Filtered] Electron Microscopy Position Available

Message: Hello,

Cold Spring Harbor Laboratory is currently seeking an electron
microscopy technician. Please see below for details.


Research Associate – Electron Microscopy Technologist

Cold Spring Harbor Laboratory seeks a highly motivated dedicated
individual to work in a state-of-the-art Microscopy Shared Resource.

The individual should have extensive practical expertise in biological
sample preparation for transmission and scanning electron microscopy.
Hands-on knowledge of confocal and widefield fluorescence microscopy
would also be a plus. The candidate will help users design innovative
experiments and they will carry out sample preparation and imaging as
well as assist in data interpretation.

Excellent verbal and written communication skills, ability to work with
multiple users in a supporting role, and ability to work independently
and proactively with limited supervision are essential. A Bachelor’s
degree in biology or related discipline is required. One to three years
of experience working in a Microscopy Shared Resource is preferred.

How to Apply

Interested individuals should apply for this position via the CSHL
careers website at https://www.cshl.edu/careers.

Position Number 01779-R

Applicants should include a resume along with a description of their
practical expertise and the names as well as email addresses of 3
references.

Cold Spring Harbor Laboratory is a world-renowned research and
educational institution recognized internationally for its excellence in
ground-breaking research programs in cancer, neuroscience, plant
biology, genomics, and bioinformatics and broad educational mission.

For more information about CSHL, please visit us at www.cshl.edu

CSHL is an EO/AA Employer. All qualified applicants will receive
consideration for employment and will not be discriminated against on
the basis of race, color, religion, sex, sexual orientation, gender
identity, national origin, age, disability or protected veteran status.
VEVRAA Federal Contractor

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From: microscopy.listserver-at-gmail.com
Date: Fri, 17 Nov 2017 17:20:11 -0600
Subject: [Microscopy] viaWWW:Job Opening - microscopist with Raman & IR experience

Contents Retrieved from Microscopy Listserver Archives
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Email: mholman-at-mvainc.com Name: Melissa Holman

Organization: MVA Scientific Consultants

Title-Subject: [Filtered] Job Opening - microscopist with Raman & IR
experience

Message: MVA Scientific Consultants, a premier microscopy and
microanalysis laboratory and consulting group located in Atlanta, GA, is
looking for a microscopist with experience in the preparation and
analysis of microscopic samples by confocal Raman microscopy and
infrared microspectroscopy with an emphasis on materials and
pharmaceutical samples. The ideal candidate will have a minimum of a
Bachelor’s degree in a science field and several years of experience.

Please contact Melissa Holman at mholman-at-mvainc.com for more information.

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From: microscopy.listserver-at-gmail.com
Date: Sat, 18 Nov 2017 20:26:52 -0600
Subject: [Microscopy] Fwd: Retirement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Glen MacDonald {glenmac-at-u.washington.edu}

Hello

I’m retiring from the University of Washington and would like to thank the members of this listserv
for the informative discussions and helpful answers to my questions over the many years that I’ve
been subscribed. An especially big thanks to Nestor for getting it started and keeping it running
for so long.

My email address will remain active. But, any inquiries regarding the Digital Microscopy Center
should be directed to my replacement, Kim Miller, kimiline-at-uw.edu; especially if you are interested
in using the resources :-)

Regards,
Glen
Glen MacDonald
Digital Microscopy Center
Box 357923
University of Washington
Seattle, WA 98195-7923 USA
(206) 616-4156
depts.washington.edu/digmicro
glenmac-at-uw.edu










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From: microscopy.listserver-at-gmail.com
Date: Sat, 18 Nov 2017 20:27:34 -0600
Subject: [Microscopy] Fwd: Re: viaWWW:EDS and SEM projector for Outreach

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Zack Gainsforth {zackg-at-berkeley.edu}

I’ve had good results showing young people fossils, shells or dinosaur bone fragments. Kids love
those! Also, teeth, and hair can be interesting. Hair is especially captivating because they can
donate it from their own head and it becomes personal. It also contains S which is a surprise to
most people.

Cheers,

Zack

} On Nov 17, 2017, at 3:39 PM, microscopy.listserver-at-gmail.com wrote:
}
}
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} Email: annie.muske-dukes-driggs-at-lonza.com Name: Annie Muske-Dukes
}
} Organization: Bend Research
}
} Title-Subject: [Filtered] EDS and SEM projector for Outreach
}
} Message: Hi everyone,
}
} My company runs a community outreach program for local elementary school
} students (8-10 years old). They tour our facility and see quick
} demonstrations/lectures on a variety of topics.
} We have had an SEM demo for years and I would like to incorporate our
} EDS but I'm having trouble coming up with samples that kids that age
} understand, especially since I only have 15 minutes per group and we try
} to go through at least four samples.
}
} I have generated a map of different elements in a vitamin tablet which
} went over well and gives them a good understanding of what the system is
} doing. Any recommendations for samples that generate interesting maps or
} have unexpected elements? I'll be doing the prep/high quality maps ahead
} of time so that doesn't need to be done in the 15 minutes.
}
} I'd also like to get a wireless projector to use with our Hitachi SU3500
} to project a live image of the screen onto the wall. Our service rep
} said some models can cause driver issues so if anyone here has a model
} that works please let me know. We need to stay under US$500, but under
} $300 would be ideal.
}
} Thanks,
} Annie (I am a list subscriber but our required corporate attachments
} prevent me from sending the usual way)
}
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From: microscopy.listserver-at-gmail.com
Date: Sun, 19 Nov 2017 08:36:12 -0600
Subject: [Microscopy] Fwd: [Filtered] EDS issue high intensity low energy peak

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Erico Freitas {freitas.erico-at-gmail.com}


Dear,


Our EDS is showing a high intensify peak at about 0.2 keV even with beam on vacuum. We're not
getting C signal anymore and the energy resolution is getting worse. There'is been an energy shift also.
The issue remains after run an automatic callibraion.
Does anybody know what more could be done? Or is it a sign that our detector is almost dead?

Regards,

Erico Freitas
Físico/Physicist
Centro de Microscopia
Universidade Federal de Minas Gerais

Coordenador do Lab. de Microscopia Eletrônica dr Transmissão

==============================Original Headers==============================
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From: brachfelds-at-mail.montclair.edu
Date: Sun, 19 Nov 2017 11:33:08 -0600
Subject: [Microscopy] Service contract providers for laboratory equipment

Contents Retrieved from Microscopy Listserver Archives
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Dear colleagues,
Does anyone have experience with companies such as LBR Scientific,
Cryostar, or other 3rd party providers of service contracts for lab
equipment (fume hoods, autoclaves, cold rooms, etc)?

I would appreciate any insight you can share, both positive and negative.
Stefanie

*********************
Dr. Stefanie Brachfeld
Acting Associate Dean for Academic Affairs, College of Science and Mathematics
Montclair State University
Montclair, NJ 07043
phone: (973) 655-5129
brachfelds-at-mail.montclair.edu
*********************

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From: microscopy.listserver-at-gmail.com
Date: Mon, 20 Nov 2017 08:10:28 -0600
Subject: [Microscopy] Fwd: viaWWW:EDS and SEM projector for Outreach

Contents Retrieved from Microscopy Listserver Archives
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X-from: jacques faerber {jacques.faerber-at-ipcms.unistra.fr}

Hi

I'got once a nice paper towel with Christmas decorations, gold, silver and black patterns on  the
blue towel. It is particulary stiff and looks very close to a real woven towel. So I kept one for
SEM practicals with students. You schould be able to find something similar.
I didn't try it with joung chidren but I imagine it could do the job. They can have the tovel in
their hands, and see in Se/BSe a synthetic/cellulose fiber network, in BSe a compositionnal
contraste image and by EDS in spectrum mode schowing that the "gold" is brass, the "silver" is
aluminum and the black is carbon... All what shines is not gold...

Best regards
Jacques


Le 18/11/2017 à 00:49, microscopy.listserver-at-gmail.com a écrit :
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} Email: annie.muske-dukes-driggs-at-lonza.com Name: Annie Muske-Dukes
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} Organization: Bend Research
}
} Title-Subject: [Filtered] EDS and SEM projector for Outreach
}
} Message: Hi everyone,
}
} My company runs a community outreach program for local elementary school
} students (8-10 years old). They tour our facility and see quick
} demonstrations/lectures on a variety of topics.
} We have had an SEM demo for years and I would like to incorporate our
} EDS but I'm having trouble coming up with samples that kids that age
} understand, especially since I only have 15 minutes per group and we try
} to go through at least four samples.
}
} I have generated a map of different elements in a vitamin tablet which
} went over well and gives them a good understanding of what the system is
} doing. Any recommendations for samples that generate interesting maps or
} have unexpected elements? I'll be doing the prep/high quality maps ahead
} of time so that doesn't need to be done in the 15 minutes.
}
} I'd also like to get a wireless projector to use with our Hitachi SU3500
} to project a live image of the screen onto the wall. Our service rep
} said some models can cause driver issues so if anyone here has a model
} that works please let me know. We need to stay under US$500, but under
} $300 would be ideal.
}
} Thanks,
} Annie (I am a list subscriber but our required corporate attachments
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--

J. Faerber
IPCMS-DSI
Institut de Physique et Chimie des Matériaux de Strasbourg
Département Surfaces et Interfaces
23, rue de Loess ; BP43
67034 Strasbourg CEDEX 2
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)3 88 10 72 48
E-mail : Jacques.Faerber-at-ipcms.unistra.fr


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From: microscopy.listserver-at-gmail.com
Date: Mon, 20 Nov 2017 08:11:56 -0600
Subject: [Microscopy] viaWWW:SEM-CL missing red wavelengths?

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X-from: Colin.Macrae-at-csiro.au

Hi Isabel;

The Gatan MonoCL uses a mirror arrangement to direct the light to the optics. It may be that the
longer wavelengths (red-orange) need a different geometry for optimisation of the collection. I
would try and move the sample height and see if the spectra sensitivity changes with respect to the
red end of the spectrum.
The MonoCl systems typically have a PMT detector to detect the light and these usually have
adjustable gain. Since the longer wavelengths are energetically weak you may have to increase the
gain to detect these less energetic wavelengths.

Regards
Colin

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To: MacRae, Colin (Mineral Resources, Clayton) {Colin.Macrae-at-csiro.au}

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Email: fay1-at-email.arizona.edu Name: Isabel Barton

Organization: University of Arizona

Title-Subject: [Filtered] SEM-CL missing red wavelengths?

Message: Dear Microscopy Community:

Does anybody know why an SEM-CL instrument could be missing the red-to-orange wavelengths of the
cathodoluminescence spectrum?

I run a JEOL 6010LA benchtop SEM wit a recently acquired Gatan MonoCL4 cathodoluminescence
spectrometer. It works great for zircons, quartz, etc., but picks up little to no signal in the red
to orange part of the spectrum even on samples that glow brilliant red under our ancient optical
cold-cathode Luminoscope. I checked the same samples out on a ChromaCL hooked up to a Hitachi S3400
in another lab, and there is a similar lack of red signal.
I've checked and the accelerating voltages on the Luminoscope are about the same as on the SEM,
although the Luminoscope has higher beam current, so I'm not sure why the SEM-CL instruments don't
pick up the red luminescence. I've read that filters placed in front of a detector can eliminate
red-orange luminescence and phosphorescence (Reed and Milliken, Journal of Sedimentary Research
73.2: 328-332). There was no mention of such a filter in the Gatan datasheets for either the
ChromaCL or the MonoCL4, but that seems to me to be one possible explanation for the lack of red
luminescence in SEM-CL compared to optical cold-cathode CL. I've left a message with Gatan but
haven't heard back yet.
Has anybody else with SEM-CL encountered a similar problem, or know of any fixes? We got the MonoCL4
partly in order to look at carbonate cements in rocks, so it's pretty important that we figure out
how to get good spectra and images in the red and orange zones.

Any help would be appreciated.
Thanks,

Isabel Barton

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From: microscopy.listserver-at-gmail.com
Date: Mon, 20 Nov 2017 08:12:19 -0600
Subject: [Microscopy] viaWWW:EDS and SEM projector for Outreach

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X-from: Gene Rodek {erodek-at-2spi.com}

Hi Annie:

It is always fun to present science to young minds!

I would suggest table salt. Nice crystal structure for morphology and an easy spectrum. I've also
done a comparison with table salt and natural salts (Sea or Himalayan) which can show differences in
both morphology and spectra.

Have fun!

Gene
Eugene Rodek
Vice President
206 Garfield Ave
West Chester, PA 19380
610-436-5400 x109
erodek-at-2spi.com
www.2spi.com
________________________________________
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Sent: Friday, November 17, 2017 6:46 PM
To: Gene Rodek

X-from: annie.muske-dukes-driggs-at-lonza.com

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Email: annie.muske-dukes-driggs-at-lonza.com Name: Annie Muske-Dukes

Organization: Bend Research

Title-Subject: [Filtered] EDS and SEM projector for Outreach

Message: Hi everyone,

My company runs a community outreach program for local elementary school
students (8-10 years old). They tour our facility and see quick
demonstrations/lectures on a variety of topics.
We have had an SEM demo for years and I would like to incorporate our
EDS but I'm having trouble coming up with samples that kids that age
understand, especially since I only have 15 minutes per group and we try
to go through at least four samples.

I have generated a map of different elements in a vitamin tablet which
went over well and gives them a good understanding of what the system is
doing. Any recommendations for samples that generate interesting maps or
have unexpected elements? I'll be doing the prep/high quality maps ahead
of time so that doesn't need to be done in the 15 minutes.

I'd also like to get a wireless projector to use with our Hitachi SU3500
to project a live image of the screen onto the wall. Our service rep
said some models can cause driver issues so if anyone here has a model
that works please let me know. We need to stay under US$500, but under
$300 would be ideal.

Thanks,
Annie (I am a list subscriber but our required corporate attachments
prevent me from sending the usual way)

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From: microscopy.listserver-at-gmail.com
Date: Mon, 20 Nov 2017 08:13:18 -0600
Subject: [Microscopy] Fwd: Re: viaWWW:EDS and SEM projector for Outreach

Contents Retrieved from Microscopy Listserver Archives
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X-from: Kathleen Pullin {kleopullin-at-email.arizona.edu}


Teeth, rocks (granite).

On Friday, November 17, 2017, {microscopy.listserver-at-gmail.com
{mailto:microscopy.listserver-at-gmail.com} } wrote:




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Email: annie.muske-dukes-driggs-at-lonza.com {javascript:;} Name: Annie Muske-Dukes

Organization: Bend Research

Title-Subject: [Filtered] EDS and SEM projector for Outreach

Message: Hi everyone,

My company runs a community outreach program for local elementary school
students (8-10 years old). They tour our facility and see quick
demonstrations/lectures on a variety of topics.
We have had an SEM demo for years and I would like to incorporate our
EDS but I'm having trouble coming up with samples that kids that age
understand, especially since I only have 15 minutes per group and we try
to go through at least four samples.

I have generated a map of different elements in a vitamin tablet which
went over well and gives them a good understanding of what the system is
doing. Any recommendations for samples that generate interesting maps or
have unexpected elements? I'll be doing the prep/high quality maps ahead
of time so that doesn't need to be done in the 15 minutes.

I'd also like to get a wireless projector to use with our Hitachi SU3500
to project a live image of the screen onto the wall. Our service rep
said some models can cause driver issues so if anyone here has a model
that works please let me know. We need to stay under US$500, but under
$300 would be ideal.

Thanks,
Annie (I am a list subscriber but our required corporate attachments
prevent me from sending the usual way)

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--
Kleo Pullin
kleopullin-at-email.arizona.edu {mailto:kleopullin-at-email.arizona.edu}
Moraga, CA

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From: oshel1pe-at-cmich.edu
Date: Monday, 20November, 2017 at 09:36
Subject: [Microscopy] viaWWW:SEM-CL missing red wavelengths?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I believe Colin is correct about finding the right focal point with the GatanCL.
We also have this detector on a Hitachi 3400, and have no problems with the red signal. However, finding the right focal point can be essential. 0.5 mm adjustment in the Z axis or less can make a big difference in signal strength.

Phil
-------------
Philip Oshel
Imaging Facility Director
Biology Department
1304 Biosciences
1455 Calumet Ct.
Central Michigan University
Mt. Pleasant, MI 48859
989 774-3576 office
989 774-7567 lab









-----Original Message-----
X-from: "microscopy.listserver-at-gmail.com" {microscopy.listserver-at-gmail.com}
Reply-To: "microscopy.listserver-at-gmail.com" {microscopy.listserver-at-gmail.com}

X-from: Colin.Macrae-at-csiro.au

Hi Isabel;

The Gatan MonoCL uses a mirror arrangement to direct the light to the optics. It may be that the
longer wavelengths (red-orange) need a different geometry for optimisation of the collection. I
would try and move the sample height and see if the spectra sensitivity changes with respect to the
red end of the spectrum.
The MonoCl systems typically have a PMT detector to detect the light and these usually have
adjustable gain. Since the longer wavelengths are energetically weak you may have to increase the
gain to detect these less energetic wavelengths.

Regards
Colin

-----Original Message-----
X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Saturday, 18
November 2017 10:49 AM
To: MacRae, Colin (Mineral Resources, Clayton) {Colin.Macrae-at-csiro.au}

X-from: fay1-at-email.arizona.edu

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Email: fay1-at-email.arizona.edu Name: Isabel Barton

Organization: University of Arizona

Title-Subject: [Filtered] SEM-CL missing red wavelengths?

Message: Dear Microscopy Community:

Does anybody know why an SEM-CL instrument could be missing the red-to-orange wavelengths of the
cathodoluminescence spectrum?

I run a JEOL 6010LA benchtop SEM wit a recently acquired Gatan MonoCL4 cathodoluminescence
spectrometer. It works great for zircons, quartz, etc., but picks up little to no signal in the red
to orange part of the spectrum even on samples that glow brilliant red under our ancient optical
cold-cathode Luminoscope. I checked the same samples out on a ChromaCL hooked up to a Hitachi S3400
in another lab, and there is a similar lack of red signal.
I've checked and the accelerating voltages on the Luminoscope are about the same as on the SEM,
although the Luminoscope has higher beam current, so I'm not sure why the SEM-CL instruments don't
pick up the red luminescence. IÂ've read that filters placed in front of a detector can eliminate
red-orange luminescence and phosphorescence (Reed and Milliken, Journal of Sedimentary Research
73.2: 328-332). There was no mention of such a filter in the Gatan datasheets for either the
ChromaCL or the MonoCL4, but that seems to me to be one possible explanation for the lack of red
luminescence in SEM-CL compared to optical cold-cathode CL. I've left a message with Gatan but
haven't heard back yet.
Has anybody else with SEM-CL encountered a similar problem, or know of any fixes? We got the MonoCL4
partly in order to look at carbonate cements in rocks, so itÂ's pretty important that we figure out
how to get good spectra and images in the red and orange zones.

Any help would be appreciated.
Thanks,

Isabel Barton

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From: sem.nattabg-at-gmail.com
Date: Mon, 20 Nov 2017 14:54:41 -0600
Subject: [Microscopy] viaWWW:EDS and SEM projector for Outreach

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Annie,
I usually show coins and matches (new and burnt) for similar purpose.
Coins for oxidation (Copper coin) and metal allergies (Ni alloy).
Matches produce nice elemental maps of Cl, K, Na.

Regards,

Elio



2017-11-18 0:53 GMT+01:00 {microscopy.listserver-at-gmail.com} :
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} Email: annie.muske-dukes-driggs-at-lonza.com Name: Annie Muske-Dukes
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} Organization: Bend Research
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} Title-Subject: [Filtered] EDS and SEM projector for Outreach
}
} Message: Hi everyone,
}
} My company runs a community outreach program for local elementary school
} students (8-10 years old). They tour our facility and see quick
} demonstrations/lectures on a variety of topics.
} We have had an SEM demo for years and I would like to incorporate our
} EDS but I'm having trouble coming up with samples that kids that age
} understand, especially since I only have 15 minutes per group and we try
} to go through at least four samples.
}
} I have generated a map of different elements in a vitamin tablet which
} went over well and gives them a good understanding of what the system is
} doing. Any recommendations for samples that generate interesting maps or
} have unexpected elements? I'll be doing the prep/high quality maps ahead
} of time so that doesn't need to be done in the 15 minutes.
}
} I'd also like to get a wireless projector to use with our Hitachi SU3500
} to project a live image of the screen onto the wall. Our service rep
} said some models can cause driver issues so if anyone here has a model
} that works please let me know. We need to stay under US$500, but under
} $300 would be ideal.
}
} Thanks,
} Annie (I am a list subscriber but our required corporate attachments
} prevent me from sending the usual way)
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From: microscopy.listserver-at-gmail.com
Date: Mon, 20 Nov 2017 19:10:32 -0600
Subject: [Microscopy] viaWWW: Optronics Labratories Manuals

Contents Retrieved from Microscopy Listserver Archives
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Email: jerry.biehler-at-gmail.com Name: Jerry Biehler

Organization: ESI

Title-Subject: [Filtered] Optronics Labratories Manuals

Message: This is a bit off topic but Im thowing this out there hoping someone might have some leads.
I have a QE system from SITe that I am trying to work some bugs out of. It is composed of a Optronic
Laboratories 740A/D Monochromater controlled with a 740-1C wavelength drive and a 730A Radiometer
amp/readout.

Trying to find the manuals or at least the controller/BCD port specs for the wavelength drive and
radiometer.

Gooch & Housego took them over but it is looking like they may not have any info on these old units
anymore.
Thanks

-Jerry

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From: microscopy.listserver-at-gmail.com
Date: Mon, 20 Nov 2017 19:36:45 -0600
Subject: [Microscopy] viaWWW:EDS and SEM projector for Outreach

Contents Retrieved from Microscopy Listserver Archives
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X-from: Greg Baty {gbaty-at-pdx.edu}


Annie,

I usually deal with kids in the 12 - 18 YO range for outreach.  In the past we offered a week long
half day experience in the lab.  In my experience it is critical to find samples that kids can
relate to.

Insects can be a good for imaging.  My favorites are honey bees, house fly's and fruit fly's.  Kids
really like the structure of the eye.

Epsom salt has a nice structure and is useful for EDX; however, it can take a long time to get
vacuum since it is usually hydrated.  Best to only use a few crystals or prepump.

Geological samples.  I prefer a thin section to reduce pump time, but bulk sections work too.  If
you need a thin section I suspect Spectrum Petrographics could help. I have no financial connection
to them, but I know several of our geology Professors have Spectrum do their mounts.

I had one student bring hair and feathers from the farm she lives on.  There is a significant
difference between animals.

House hold dust can be a good sample.  Just pick a spot like a window sill and don't clean it for a
week or two.  Collect the sample with C tape on a stub.  I have seen pollen, insect parts and
silicate minerals.

Greg

On Fri, Nov 17, 2017 at 3:49 PM, {microscopy.listserver-at-gmail.com
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Email: annie.muske-dukes-driggs-at-lonza.com {mailto:annie.muske-dukes-driggs-at-lonza.com} Name:
Annie Muske-Dukes

Organization: Bend Research

Title-Subject: [Filtered] EDS and SEM projector for Outreach

Message: Hi everyone,

My company runs a community outreach program for local elementary school
students (8-10 years old). They tour our facility and see quick
demonstrations/lectures on a variety of topics.
We have had an SEM demo for years and I would like to incorporate our
EDS but I'm having trouble coming up with samples that kids that age
understand, especially since I only have 15 minutes per group and we try
to go through at least four samples.

I have generated a map of different elements in a vitamin tablet which
went over well and gives them a good understanding of what the system is
doing. Any recommendations for samples that generate interesting maps or
have unexpected elements? I'll be doing the prep/high quality maps ahead
of time so that doesn't need to be done in the 15 minutes.

I'd also like to get a wireless projector to use with our Hitachi SU3500
to project a live image of the screen onto the wall. Our service rep
said some models can cause driver issues so if anyone here has a model
that works please let me know. We need to stay under US$500, but under
$300 would be ideal.

Thanks,
Annie (I am a list subscriber but our required corporate attachments
prevent me from sending the usual way)

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--
Greg Baty
Center for Electron Microscopy and Nanofabrication
Portland State University
503-725-2867
www.pdx.edu/cemn {http://www.pdx.edu/cemn}

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From: microscopy.listserver-at-gmail.com
Date: Mon, 20 Nov 2017 20:26:24 -0600
Subject: [Microscopy] Fwd: Re: EDS issue high intensity low energy peak

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Julian Smith III {smithj-at-winthrop.edu}

X-from: Ferenc Molnar {ferenc.l.molnar-at-googlemail.com}


Dear Erico,

this sounds to me as there is an issue with additional light in the chamber. Make sure that all
light sources - especially chamber scope - are turned off. A little bit tricky might be a touch
alarm: on some SEM (do we talk about SEM?) the stage alarm triggers some safety light barrier in
order to prevent another collision. To turn these lights off, you have to initialize the stage.

If you can exclude additional light in the chamber, there is maybe an issue with the detector: it
can be the cooling system or some issue with the preamp of the SDD/SiLi.

I hope that helps. In general, EDS detectors are very sensible to all kind of light, not only the
Xray you want to see.


With best regards,
Ferenc

{microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } schrieb am So., 19. Nov.
2017 um 16:38 Uhr:




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X-from:         Erico Freitas {freitas.erico-at-gmail.com {mailto:freitas.erico-at-gmail.com} }


Dear,


Our EDS is showing a high intensify peak at about 0.2 keV even with beam on vacuum. We're not
getting C signal anymore and the energy resolution is getting worse. There'is been an energy
shift also.
The issue remains after run an automatic callibraion.
Does anybody know what more could be done? Or is it a sign that our detector is almost dead?

Regards,

Erico Freitas
Físico/Physicist
Centro de Microscopia
Universidade Federal de Minas Gerais

Coordenador do Lab. de Microscopia Eletrônica dr Transmissão

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--
Ferenc Molnar
Physiker / physicist
Schwetzingen (Germany)

https://de.linkedin.com/in/ferenc-molnar-5792b7105
https://www.xing.com/profile/Ferenc_Molnar4?sc_o=mxb_p

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From: microscopy.listserver-at-gmail.com
Date: Mon, 20 Nov 2017 20:27:30 -0600
Subject: [Microscopy] Resource needed Electron beam lithography and Ion Beam Lithography

Contents Retrieved from Microscopy Listserver Archives
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X-from: Mainak Palit {mainakpalit-at-gmail.com}


Hi everyone!

Just a simple help-

Please suggest a good resource for Electron beam lithography and Ion Beam Lithography!
In the form of a book or a website or a document repository!

Regards.
Mainak

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From: colijn.1-at-osu.edu
Date: Tue, 21 Nov 2017 07:44:53 -0600
Subject: [Microscopy] viaWWW:EDS and SEM projector for Outreach

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Annie, et al.

I did a CSI type demo using light bulb filaments. The scenario was that
there was a traffic accident in the dark and each driver claimed the
others headlights were not on. The headlamps in both cars were broken.
The 2nd driver claims that the 1st driver turned on his headlights after
the bulbs were broken in order to cover his fault. The officer collected
the light bulb filaments from the cars and you are comparing them in the
SEM. (You can spin out the story as much as you want!)

I took 2 small incandescent bulbs (15 watt if I remember), broke the
glass on one and then applied power so that it burned out. I took the
2nd and broke the glass while it was running. I then mounted the 2
filaments on an SEM sticky tab and put them in the scope.

Note: be careful about breaking the glass. Do it safely! I wore
safety glasses and leather gloves and used a pair of channel pliers to
hold the bulb inside a mostly closed cardboard box to contain the glass
fragments.

On examination, the student will find small spherical globs on one
filament and none on the other. Doing EDX shows that the spherical
globs are Si and O (i.e. glass that melted). The inference is that the
filament was hot when the glass was broken, hence the light bulb was on.
The other bulb is just oxidized and doesn't show any melted glass.
Hence it was not running when the glass broke and was burned out
afterwards.

They seemed to enjoy the "real life" problem solving.

Cheers,
Henk




--------------------

Hendrik O. Colijn
Center for Electron Microscopy and AnalysiS
The Ohio State University
1305 Kinnear Rd, Suite 100, Columbus, OH 43212

colijn.1-at-osu.edu 614/643-3458
cemas.osu.edu

"Time is that quality of nature which keeps things from happening all at
once." (Ray Cummings - 1922)
Lately it doesn't seem to be working.

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18, 125 -- Subject: Re: [Microscopy] viaWWW:EDS and SEM projector for Outreach
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From: microscopy.listserver-at-gmail.com
Date: Wed, 22 Nov 2017 06:37:22 -0600
Subject: [Microscopy] viaWWW:Job Vacancy - Associate Director, Electron Microcope Unit -

Contents Retrieved from Microscopy Listserver Archives
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I just found these on Ebay- is there anyone out there with the passion/free time/garage space to rescue these before they get scrapped? I just hate seeing these old scopes go to scrappers…

https://www.ebay.com/itm/Vintage-RCA-Electron-Microscopes-EMU-3-EMU-4/332447246760?hash=item4d676669a8:g:JGQAAOSwz~paCKhQ

—Justin A. Kraft

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From carlemau296wvuy-at-gmail.com Wed Nov 22 04:33:03 2017
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Email: shiree.thomas-at-unsw.edu.au Name: Shiree Thomas

Organization: UNSW Sydney

Title-Subject: [Filtered] Job Vacancy - Associate Director, Electron Microcope Unit - Sydney, Australia

Message: Academic and Associate Director level position for candidate with electron microscopy
expertise and independent research portfolio (materials science preferred)at UNSW Sydney.
• Associate Professor/Senior Lecture level five-year appointment with options for renewal
• Make a real impact! Influence and support advancements in electron microscopy
• Enjoy state-of-the-art facilities and collaboration with interdisciplinary researchers

For further information and to view the position description please click on the link below:
https://applicant.cghrm.unsw.edu.au/psp/hrm/NS_CAREERS/HRMS/c/HRS_HRAM.HRS_APP_SCHJOB.GBL?Page=HRS_APP_JBPST&Action=U&FOCUS=Applicant&SiteId=1&JobOpeningId=58234&PostingSeq=1

Contact:
Professor Richard Tilley
Director – Electron Microscope Unit and AMMRF node
Mark Wainwright Analytical Centre, Division of Research T +61 (2) 9385 4435
E r.tilley-at-unsw.edu.au



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From: microscopy.listserver-at-gmail.com
Date: Wed, 22 Nov 2017 18:55:11 -0600
Subject: [Microscopy] viaWWW:Application Scientist - Cathodoluminescence (CL) Job Opening

Contents Retrieved from Microscopy Listserver Archives
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X-from: jhyun-at-gatan.com

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Email: jhyun-at-gatan.com Name: John Hyun

Organization: Gatan

Title-Subject: [Filtered] Application Scientist - Cathodoluminescence (CL) Job Opening

Message: Gatan, Inc. is currently seeking a skilled CL Application Scientist who will be responsible
for supporting the sales and marketing efforts of Gatan’s highly successful Cathodoluminescence (CL)
product line. The position is based at Gatan’s Pleasanton, CA headquarters but will support CL
products worldwide.

Primary duties of this role will include:

• Providing pre-sales applications support with presentations and demonstrations of Gatan products
on a range of SEM and TEM models.
• Driving the development of new applications by working closely with key customers, presenting at
scientific conferences and writing scientific articles.
• Providing post-sales customer support and on-site customer training. Tasks include: general
applications support, phone/email support, and limited hardware and software troubleshooting.
• Proposing design enhancements and improvements to Gatan hardware and software.
• Working with Gatan R&D in the development and testing of new products and applications.
The successful incumbent will have:
• Ability to interface effectively with customers at all experience levels while projecting a strong
client service attitude.
• Excellent verbal, written, and interpersonal communication skills in English are essential.
• Ability to work on complex learning and development problems as well as teach highly technical
information is essential.

Requirements:
• Advanced degree in science or engineering (or equivalent experience) is required.
• Strong background in electron microscopy (SEM and/or TEM), specifically in relation to the
acquisition, application, interpretation and, discussion of CL.
• Hands-on, post-graduate level experience in advanced CL applications with a proven publication record.
• Ability to manage approximately 50% both domestic and international travel required.
To apply to this opportunity or to view our other current job openings, please visit
www.gatan.com/careers.
Equal Opportunity Employer/Protected Veterans/Individuals with Disabilities


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From: microscopy.listserver-at-gmail.com
Date: Thu, 23 Nov 2017 07:57:42 -0600
Subject: [Microscopy] Fwd: Biological TEM specialist positon at KAUST

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X-from: simon lee {kunli218-at-yahoo.com.sg}




There is a permanent TEM specialist position opening at the Imaging and Characterization Core Lab of
King Abdullah University of Science and Technology (KAUST) in Saudi Arabia. Please find the detailed
information below. KASUT offers competitive package and balanced work/life environment. If
interested, please send your CV to kun.li-at-kaust.edu.sa

Regards,

Kun Li

King Abdullah University of Science and Technology is an international graduate-level, merit-based
research university located on the shores of the Red Sea in Saudi Arabia. Our state-of-the-art
campus, globally renowned faculty and cutting-edge facilities come together to provide the ideal
setting for significant, high-impact research. KAUST is dedicated to inspiring a new age of
scientific achievement in the Kingdom that will also benefit the region and the world.

*Major Responsibilities *
·Perform standardized protocols for histology
·Handle research specimens based on sound knowledge of histological/immunochemical processes.
·TEM sample preparation and TEM imaging with focus in the field of biology
·Maintain and run EM preparation lab
·Engage in KAUST research projects with senior staff to provide value-added service
·Ensure maintenance status and maximum uptime of assigned instruments (plan, perform and oversee
preventative and corrective maintenance, purchase consumables, etc.)
·Keep safe working procedure, in compliance with KAUST HSE regulations.
·Other duties as assigned by the team lead and lab director
*Competencies *
·Good knowledge of cell biology and cell structure
·General understanding and expertise in cell/tissue fixation, processing, embedding, sectioning and
staining.
·Ability to operate common sample preparation tools independently
·Hands-on experience on EM sample preparation
·Hands-on experience on Ultramicrotome
·Working experience with cell culture
·Experience with cryo electron microscopy preferred
·Good communication and inter-personal skills
*Qualifications*
·A master’s degree in the life science or biochemistry-related field

==============================Original Headers==============================
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From: eikonika-at-otenet.gr
Date: Wed, 29 Nov 2017 02:57:57 -0600
Subject: [Microscopy] JSM5600LV burns filaments immediately -solved

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Email: Ziqiang.Huang-at-cruk.cam.ac.uk Name: Ziqiang Huang

Organization: CRUK Cambridge Institute

Title-Subject: [Filtered] FIBSEM Zeiss Orion biological applications

Message: Hi, everyone,

Does any of you have experience (or just an idea will be also helpful) using Zeiss Orion in biology
(or life science) researches, preferably in
cancer research? I used the Zeiss Auriga system before to quantify synapses in a small brain volume,
but not very familiar with the Orion system.
I will be very interested in knowing what kind of biology projects the Orion system can potentially
help.

Best Regards.
Ziqiang

Bioimage Analyst of Light Microscopy Facility
CRUK Cambridge Institute

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From jeverett397rxqi-at-gmail.com Sat Nov 25 16:26:06 2017
Return-Path: {jeverett397rxqi-at-gmail.com}
Received: from gmail.com ([121.201.38.118])
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Dear List
Thanks to everybody provided ideas about the problem. We found a faulty
voltage regulator in the HT I/O board. This regulator provides a 39V power
rail to the HT Drive board, used by the filament current generating circuit.
The regulator had failed short resulting in an overvoltage of some 70V going
to the filament circuitry, probably causing more damage there (we replaced
the regulator but it was destroyed instantly). Finally we replaced the
faulty voltage regulator, the HT drive board and the HV power supply in the
oil tank. I was lucky to have spares of the last two. The microscope now
works fine.

Only thing that we still try to solve is that the readings on the SEM menu
regarding gun alignment values seem to be incorrect. Also when we turn HT
on, the value of the current fluctuates for several seconds before
stabilizing to the expected value. Since the HT board and power supply are
coming from another microscope, it may need some sort of reset that we
haven't found yet. Has anybody any clue?

Best regards
yorgos



Dear List
Our SEM JSM5600LV burns filaments immediately upon turning high tension ON.
There was a thunderstorm here and after a big thunder the microscope went
completely OFF while there was no power cut. Very soon the microscope's
power came back and worked normally but I noticed the image was bit noisy
and definition less high. Then I switched it off overnight and today I
started and realized the problem (after I burned a couple of filaments). Any
comment or suggestion will be greatly appreciated

yorgos

Dr Yorgos Nikas
Athens Innovative Microscopy
Skra 36 Voula 16673 GREECE

Tel/fax +30 210 8957677
mobile +30 6945 107477
www.eikonika.net www.aim.cat
*************************************



==============================Original Headers==============================
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From: zaluzec-at-microscopy.com
Date: Wed, 29 Nov 2017 09:29:02 -0600
Subject: [Microscopy] viaWWW:Aurion Immuno Gold Silver Staining Workshop

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X-from: sgkcck-at-aol.com


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Email: sgkcck-at-aol.com Name: Stacie Kirsch

Organization: Electron Microscopy Sciences

Title-Subject: [Filtered] Aurion Immuno Gold Silver Staining Workshop

Message: Electron Microscopy Sciences is pleased to offer the Aurion Immuno Gold Silver Staining
Workshop, led by expert Peter van de Plas, at our new facility - the EMS Microscopy Academy!

Details:
Aurion Immuno Gold Silver Staining Workshop
Tuesday - Thursday
February 20 - 22, 2018
8:00 a.m. - 4:30 p.m.
Hatfield, Pennsylvania, USA

The objective of the course is to provide researchers with the opportunity to learn the theory and
practice of Immuno Gold labeling. Participants will process their own samples under the expert
guidance of our tutors, who are experts in Immuno Gold Silver Staining techniques.

During the workshop attendees will receive theory, including but not limited to immune detection, in
situ hybridization, silver enhancement, as well as background issues. There will be time for
practice, as well. Attendees will be able to work with their own specimens, as well as ones we will
have prepared. A full review of incubation methods, testing of antigenicity and reactivity, complete
principles of Immuno Gold labeling, and preparation of conjugates for EM and LM will be covered.
For more information, copy and paste this link into your browser!
http://www.emsdiasum.com/microscopy/academy/courses/immunogold2.aspx

or contact me at sgkcck-at-aol.com

Thank you for your support!

Stacie Kirsch

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From: microscopy.listserver-at-gmail.com
Date: Wed, 29 Nov 2017 09:29:50 -0600
Subject: [Microscopy] viaWWW:Research Associate - Electron Microscopy Technologist

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Email: walbrid-at-cshl.edu Name: Samantha

Organization: Cold Spring Harbor Laboratory

Title-Subject: [Filtered] Research Associate - Electron Microscopy Technologist

Message: Cold Spring Harbor Laboratory seeks a highly motivated dedicated individual to work in a
state-of-the-art Microscopy Shared Resource.

The individual should have extensive practical expertise in biological sample preparation for
transmission and scanning electron microscopy of animal tissues and mammalian cell lines. Hands-on
knowledge of confocal and widefield fluorescence microscopy would also be a plus.

The candidate will help users design innovative experiments and they will carry out sample
preparation and imaging as well as assist in data interpretation.

Excellent verbal and written communication skills, ability to work with multiple users in a
supporting role, and ability to work independently and proactively with limited supervision are
essential. A Bachelor’s degree in biology or related discipline is required. One to three years of
experience working in a Microscopy Shared Resource is preferred.

How to Apply
Interested individuals should apply for this position via the CSHL careers website at
http://cshl.peopleadmin.com/postings/11688

Position Number 01779-R

Applicants should include a resume along with a description of their practical expertise and the
names as well as email addresses of 3 references.

Cold Spring Harbor Laboratory is a world-renowned research and educational institution recognized
internationally for its excellence in ground-breaking research programs in cancer, neuroscience,
plant biology, genomics, and bioinformatics and broad educational mission.

For more information about CSHL, please visit us at www.cshl.edu

CSHL is an EO/AA Employer. All qualified applicants will receive consideration for employment and
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From: steven.spurgeon-at-pnnl.gov
Date: Wed, 29 Nov 2017 19:17:36 -0600
Subject: [Microscopy] Spray on foam sound insulation?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello everyone,

We’re currently in the process of setting up a new JEOL GrandARM instrument and we are looking at spray on foam insulation as a possible way to dampen sound inside the scope room. I have found several different manufacturers focused on reducing sound in recording studios, auditoriums, etc.

Does anyone have experience with the use of such insulation? How effective is it and how does it compare to sound dampening ceiling tiles (e.g. tectum)?

Thanks for your help!
______________________________________
Steven R. Spurgeon, Ph.D.
Staff Scientist
Energy and Environment Directorate

Pacific Northwest National Laboratory
902 Battelle Boulevard
P.O. Box 999 MSIN:P7-25
Richland, WA 99352

Tel: +1-509-371-7709
steven.spurgeon-at-pnnl.gov
www.stevenspurgeon.com {http://www.stevenspurgeon.com/}
www.pnnl.gov {http://www.pnnl.gov/}



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From: microscopy.listserver-at-gmail.com
Date: Thu, 30 Nov 2017 07:42:16 -0600
Subject: [Microscopy] viaWWW:Gatan Disc Grinder 623 Procedure?

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Email: keith.prater-at-solvay.com Name: Keith Prater

Organization: Solvay Specialty Polymers

Title-Subject: [Filtered] Gatan Disc Grinder 623 Procedure?

Message: Dear Listers,

Does anyone have a detailed procedure that you'd be willing and allowed to share for pre-thinning
specimens using the Gatan Disc Grinder 623? It would be very much appreciated as I have found only
limited descriptions online.

Thank you!
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From: microscopy.listserver-at-gmail.com
Date: Thu, 30 Nov 2017 07:43:58 -0600
Subject: [Microscopy] viaWWW: Join our Team! Ted Pella, Inc.

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Email: bonnie_salyer-at-tedpella.com
Name: Bonnie Salyer

Organization: Ted Pella, Inc.

Title-Subject: [Filtered] Join our Team!
Message: Live and work in one of the most beautiful areas in California – Redding. Ted Pella Inc. is
seeking a Materials Science Product Specialist to be responsible product development and
sales/marketing activities related to our SEM, Materials Science, Forensic and AFM product lines.
This is an exciting time for this position as we recently acquired additional product lines
associated with the semi-conductor industry. Must have BS in Materials Science or related, 5 years
hands on SEM instrument use and specimen prep experience, and 2 years TEM instrument use and
specimen prep experience. To apply email letter of interest, resume and salary requirements to
human_resources-at-tedpella.com. See our full job posting at www.tedpella.com
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From: jkrupp-at-deltacollege.edu
Date: Thu, 30 Nov 2017 15:19:20 -0600
Subject: [Microscopy] Instructor at Delta College

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The position at Delta College, Stockton, California, has been posted:

Associate Professor/Professor of Electron Microscopy

Salary: $51,912.00 - $106,386.00 Annually
Location: Stockton, CA
Job Type: Full Time
Department: Applied Science, Business & Technology
Job Number: 11-30-17
Closing: 12/8/2017 5:00 PM Pacific

Description
Under the general supervision of the Division Dean of Applied Science, Business and Technology, the Associate Professor/Professor of Electron Microscopy teaches classes primarily in the electron microscopy discipline; the primary responsibility is to teach 15 units (per term) in the assigned discipline.

This is a one year temporary position, full time. Start date: January 2018

For more info follow this link:

https://www.governmentjobs.com/careers/deltacollege . It’s on page 4

NB: This is a one year position. The HR guys waited until the last minute and couldn’t get it together to do a full search for a permanent appointment. That is supposed to happen during next year. This closes in a week, so do not delay if you are interested. I can answer any questions via email.

Jon

Jonathan Krupp
ASB&T
San Joaquin Delta College
5151Pacific Ave.
Stockton, CA 95207
(209) 954-5284
jkrupp-at-deltacollege.edu

After 12/30/17 my email will be:
jkrupp267-at-gmail.com




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From: microscopy.listserver-at-gmail.com
Date: Thu, 30 Nov 2017 20:12:46 -0600
Subject: [Microscopy] Re: viaWWW:EDS and SEM projector for Outreach

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Elaine Humphrey {ech-at-uvic.ca}
To: annie.muske-dukes-driggs-at-lonza.com {annie.muske-dukes-driggs-at-lonza.com}

Hi Annie and Henk
Thank you so much for this question. I am always in need of good ideas.

We usually have a Solve the Mystery in the Family Affair, which is changing its name this year to
Microscopic Explorations, for the delegates families and friends at the M&M conference.

The pattern has developed whereby in a session on the Wednesday afternoon, it typically starts with
an introduction and then a lot of hands on activities using loupes and light microscopes adapted
from the GEMS Microscopical Explorations activities. At least three of the stations have a “solve
the mystery.” At the next meeting in Baltimore we are developing one step further and we hope to
have Fold scopes and micro meteorites.

Then the participants go to the vendor hall where we usually have access to four sems to “Solve the
Mystery”.

I have tried to correlate the mystery with the place and change it each year. For instance the CSI
Albuquerque story was: The Governor of New Mexico is coming to town. He is a baseball fan and his
favorite team is the Albuquerque Isotopes.
He was going to have his photograph taken with the cup the Isotopes just won! But disaster struck!
Someone stole the cup! It is very important we get the cup back. We have to find out who did it.
The thief left several clues at the scene of the crime:
Some pollen
Some hair
Some sand
Some fibres

He/she wasn’t a very good thief!



There were the six suspects. We decided we could not use real people as suspects so we chose to go
with Harry Potter villains
Delores Umbridge
When she was interviewed Delores Umbridge was wearing a red flower. She has brown straight hair; She
had sandy shoes because she played golf and her golf ball went into a sandy bunker; She was dressed
in pink clothes.
Bellatrix Lestrange

When she was interviewed: Bellatrix Lestrange was wearing a pink flower; she has brown straight
hair; she had sandy shoes because she was in the garden; she was wearing blue clothes.
Draco Malfoy
When he was interviewed Draco Malfoy was wearing a lily flower; he has blond straight hair; he had
sandy shoes because he took a short cut through the sand box in the garden; he was wearing grey clothes.
Voldemort

When he was interviewed: Voldemort was wearing a red flower; no hair; he had sandy shoes because he
went hiking along a trail in California; he was wearing grey clothes.
Lucius Malfoy

When he was interviewed: Lucius Malfoy was wearing a pink flower; he has blond straight hair; he had
sandy shoes because he went hiking along a trail in California; he was wearing black clothes
Severus Snape

When he was interviewed: Severus Snape was wearing a lily flower; he has straight grey hair; he
had sandy shoes because he played golf and his golf ball went into a sandy bunker; he was wearing
black clothes



Mostly the clues fit whatever materials we had, such as what three flowers with lots of pollen were
available to us that day. Two suspects for each clue.


We didn’t want one clue to give the suspect away. It had to be two clues. Each group took on one
type of material. On the stub there were three possibles and the actual to compare. So we had a
flipchart at headquarters (the Outreach Booth) with the suspects down one side .and clues along the
top. As each of the four groups got their answer they put a tick in the right box. It worked just fine.

In Portland we had access to an X-ray analysis sem. The story was:

Someone came to the desk in the Museum and handed an envelope over to the cashier. Written on the
envelope was “ Put all the cash in the envelope and don’t say anything. I’ve got a gun!”
She complied but followed him to see where he went. He took a bicycle and went through a
construction site (sand), shaped the bike along a wall (metal for X-ray analysis), over a flower bed
(pollen) which had thorny rose bushes (fibres). By that evening the police had four suspects each
with sandy shoes and bike wheels, different materials making up the bikes etc etc.

In Nashville: Someone stole a very valuable Elvis guitar and put it up for sale clandestinely. One
of the potential buyers wanted it authenticated by identifying the strings by X-ray analysis. I went
to our local music store and asked if they had any strings from 1960s. They gave me one and I bought
a new one. The composition is very different and easy to identify by X-ray analysis.

Now I need a story for Baltimore. I’ve never been to Baltimore. Is there something special we can
use in a story?
Best wishes

Elaine

Dr. Elaine C. Humphrey
Advanced Microscopy Facility
Bob Wright Science Centre A015
University of Victoria, Canada
Lab: 250-853-3968
cell: 250-886-2068
website: http://www.stehm.uvic.ca









} Hi Annie, et al.
}
} I did a CSI type demo using light bulb filaments. The scenario was that
} there was a traffic accident in the dark and each driver claimed the
} others headlights were not on. The headlamps in both cars were broken.
} The 2nd driver claims that the 1st driver turned on his headlights after
} the bulbs were broken in order to cover his fault. The officer collected
} the light bulb filaments from the cars and you are comparing them in the
} SEM. (You can spin out the story as much as you want!)
}
} I took 2 small incandescent bulbs (15 watt if I remember), broke the
} glass on one and then applied power so that it burned out. I took the
} 2nd and broke the glass while it was running. I then mounted the 2
} filaments on an SEM sticky tab and put them in the scope.
}
} Note: be careful about breaking the glass. Do it safely! I wore
} safety glasses and leather gloves and used a pair of channel pliers to
} hold the bulb inside a mostly closed cardboard box to contain the glass
} fragments.
}
} On examination, the student will find small spherical globs on one
} filament and none on the other. Doing EDX shows that the spherical
} globs are Si and O (i.e. glass that melted). The inference is that the
} filament was hot when the glass was broken, hence the light bulb was on.
} The other bulb is just oxidized and doesn't show any melted glass.
} Hence it was not running when the glass broke and was burned out
} afterwards.
}
} They seemed to enjoy the "real life" problem solving.
}
} Cheers,
} Henk
}
}
}
}
} --------------------
}
} Hendrik O. Colijn
} Center for Electron Microscopy and AnalysiS
} The Ohio State University
} 1305 Kinnear Rd, Suite 100, Columbus, OH 43212
}
} colijn.1-at-osu.edu 614/643-3458
} cemas.osu.edu
}
} "Time is that quality of nature which keeps things from happening all at
} once." (Ray Cummings - 1922)
} Lately it doesn't seem to be working.
}
} ------ Original Message ------
} X-from: microscopy.listserver-at-gmail.com
} To: colijn.1-at-osu.edu
} Sent: 11/20/2017 8:38:57 PM
} Subject: [Microscopy] viaWWW:EDS and SEM projector for Outreach
}
} }
} }
} }
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } America

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From: microscopy.listserver-at-gmail.com
Date: Fri, 1 Dec 2017 06:50:06 -0600
Subject: [Microscopy] Fwd: viaWWW:EDS and SEM projector for Outreach

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Colijn, Hendrik {colijn.1-at-osu.edu}



Edgar Allan Poe theme?
The Murders in the Rue Morgue?
Probably not "the pit and the pendulum" or "the cask of amontillado"

Not sure what you could do with crab cakes! I believe that McCormick's has a large spice facility
near the harbor front. We could smell it the last time we were there.

Cheers,
Henk

Sent from my Verizon 4G LTE Smartphone

------ Original message------
*From: *microscopy.listserver-at-gmail.com
*Date: *Thu, Nov 30, 2017 9:12 PM
*To: *Colijn, Hendrik;
*Cc: *
*Subject:*[Microscopy] Re: viaWWW:EDS and SEM projector for Outreach




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X-from: Elaine Humphrey {ech-at-uvic.ca}
To: annie.muske-dukes-driggs-at-lonza.com {annie.muske-dukes-driggs-at-lonza.com}

Hi Annie and Henk
Thank you so much for this question. I am always in need of good ideas.

We usually have a Solve the Mystery in the Family Affair, which is changing its name this year to
Microscopic Explorations, for the delegates families and friends at the M&M conference.

The pattern has developed whereby in a session on the Wednesday afternoon, it typically starts with
an introduction and then a lot of hands on activities using loupes and light microscopes adapted
from the GEMS Microscopical Explorations activities. At least three of the stations have a solve
the mystery. At the next meeting in Baltimore we are developing one step further and we hope to
have Fold scopes and micro meteorites.

Then the participants go to the vendor hall where we usually have access to four sems to Solve the
Mystery.

I have tried to correlate the mystery with the place and change it each year. For instance the CSI
Albuquerque story was: The Governor of New Mexico is coming to town. He is a baseball fan and his
favorite team is the Albuquerque Isotopes.
He was going to have his photograph taken with the cup the Isotopes just won! But disaster struck!
Someone stole the cup! It is very important we get the cup back. We have to find out who did it.
The thief left several clues at the scene of the crime:
Some pollen
Some hair
Some sand
Some fibres

He/she wasnt a very good thief!



There were the six suspects. We decided we could not use real people as suspects so we chose to go
with Harry Potter villains
Delores Umbridge
When she was interviewed Delores Umbridge was wearing a red flower. She has brown straight hair; She
had sandy shoes because she played golf and her golf ball went into a sandy bunker; She was dressed
in pink clothes.
Bellatrix Lestrange

When she was interviewed: Bellatrix Lestrange was wearing a pink flower; she has brown straight
hair; she had sandy shoes because she was in the garden; she was wearing blue clothes.
Draco Malfoy
When he was interviewed Draco Malfoy was wearing a lily flower; he has blond straight hair; he had
sandy shoes because he took a short cut through the sand box in the garden; he was wearing grey clothes.
Voldemort

When he was interviewed: Voldemort was wearing a red flower; no hair; he had sandy shoes because he
went hiking along a trail in California; he was wearing grey clothes.
Lucius Malfoy

When he was interviewed: Lucius Malfoy was wearing a pink flower; he has blond straight hair; he had
sandy shoes because he went hiking along a trail in California; he was wearing black clothes
Severus Snape

When he was interviewed: Severus Snape was wearing a lily flower; he has straight grey hair; he
had sandy shoes because he played golf and his golf ball went into a sandy bunker; he was wearing
black clothes



Mostly the clues fit whatever materials we had, such as what three flowers with lots of pollen were
available to us that day. Two suspects for each clue.


We didnt want one clue to give the suspect away. It had to be two clues. Each group took on one
type of material. On the stub there were three possibles and the actual to compare. So we had a
flipchart at headquarters (the Outreach Booth) with the suspects down one side .and clues along the
top. As each of the four groups got their answer they put a tick in the right box. It worked just fine.

In Portland we had access to an X-ray analysis sem. The story was:

Someone came to the desk in the Museum and handed an envelope over to the cashier. Written on the
envelope was Put all the cash in the envelope and dont say anything. Ive got a gun!
She complied but followed him to see where he went. He took a bicycle and went through a
construction site (sand), shaped the bike along a wall (metal for X-ray analysis), over a flower bed
(pollen) which had thorny rose bushes (fibres). By that evening the police had four suspects each
with sandy shoes and bike wheels, different materials making up the bikes etc etc.

In Nashville: Someone stole a very valuable Elvis guitar and put it up for sale clandestinely. One
of the potential buyers wanted it authenticated by identifying the strings by X-ray analysis. I went
to our local music store and asked if they had any strings from 1960s. They gave me one and I bought
a new one. The composition is very different and easy to identify by X-ray analysis.

Now I need a story for Baltimore. Ive never been to Baltimore. Is there something special we can
use in a story?
Best wishes

Elaine

Dr. Elaine C. Humphrey
Advanced Microscopy Facility
Bob Wright Science Centre A015
University of Victoria, Canada
Lab: 250-853-3968
cell: 250-886-2068
website: http://www.stehm.uvic.ca









} Hi Annie, et al.
}
} I did a CSI type demo using light bulb filaments. The scenario was that
} there was a traffic accident in the dark and each driver claimed the
} others headlights were not on. The headlamps in both cars were broken.
} The 2nd driver claims that the 1st driver turned on his headlights after
} the bulbs were broken in order to cover his fault. The officer collected
} the light bulb filaments from the cars and you are comparing them in the
} SEM. (You can spin out the story as much as you want!)
}
} I took 2 small incandescent bulbs (15 watt if I remember), broke the
} glass on one and then applied power so that it burned out. I took the
} 2nd and broke the glass while it was running. I then mounted the 2
} filaments on an SEM sticky tab and put them in the scope.
}
} Note: be careful about breaking the glass. Do it safely! I wore
} safety glasses and leather gloves and used a pair of channel pliers to
} hold the bulb inside a mostly closed cardboard box to contain the glass
} fragments.
}
} On examination, the student will find small spherical globs on one
} filament and none on the other. Doing EDX shows that the spherical
} globs are Si and O (i.e. glass that melted). The inference is that the
} filament was hot when the glass was broken, hence the light bulb was on.
} The other bulb is just oxidized and doesn't show any melted glass.
} Hence it was not running when the glass broke and was burned out
} afterwards.
}
} They seemed to enjoy the "real life" problem solving.
}
} Cheers,
} Henk
}
}
}
}
} --------------------
}
} Hendrik O. Colijn
} Center for Electron Microscopy and AnalysiS
} The Ohio State University
} 1305 Kinnear Rd, Suite 100, Columbus, OH 43212
}
} colijn.1-at-osu.edu 614/643-3458
} cemas.osu.edu
}
} "Time is that quality of nature which keeps things from happening all at
} once." (Ray Cummings - 1922)
} Lately it doesn't seem to be working.
}
} ------ Original Message ------
} X-from: microscopy.listserver-at-gmail.com
} To: colijn.1-at-osu.edu
} Sent: 11/20/2017 8:38:57 PM
} Subject: [Microscopy] viaWWW:EDS and SEM projector for Outreach
}
} }
} }
} }
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } America

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From: microscopy.listserver-at-gmail.com
Date: Fri, 1 Dec 2017 06:51:09 -0600
Subject: [Microscopy] Re: viaWWW:EDS and SEM projector for Outreach

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Nima Nikpoor Badr {14nnb1-at-queensu.ca}



Hello Dr. Humphrey,

Micheal Phelps, the American sports champion, was born in Baltimore. You can make some plans related
to his medals!

A number of metallic medals with surface patterns can be designed, cut, and painted in yellow or
silver or bronze colours, similar to those awarded in sports competitions.

The question can be "which medal belongs to Michael Phelps (if any) and which one is fake?"

Details can be designed in a way that participants need to do microscopy work and chemical analysis
to find out the answer!


Best regards,


Nima,
Queen's University







-------- Original Message --------

X-from: Elaine Humphrey {ech-at-uvic.ca}
To: annie.muske-dukes-driggs-at-lonza.com {annie.muske-dukes-driggs-at-lonza.com}

Hi Annie and Henk
Thank you so much for this question. I am always in need of good ideas.

We usually have a Solve the Mystery in the Family Affair, which is changing its name this year to
Microscopic Explorations, for the delegates families and friends at the M&M conference.

The pattern has developed whereby in a session on the Wednesday afternoon, it typically starts with
an introduction and then a lot of hands on activities using loupes and light microscopes adapted
from the GEMS Microscopical Explorations activities. At least three of the stations have a solve
the mystery. At the next meeting in Baltimore we are developing one step further and we hope to
have Fold scopes and micro meteorites.

Then the participants go to the vendor hall where we usually have access to four sems to Solve the
Mystery.

I have tried to correlate the mystery with the place and change it each year. For instance the CSI
Albuquerque story was: The Governor of New Mexico is coming to town. He is a baseball fan and his
favorite team is the Albuquerque Isotopes.
He was going to have his photograph taken with the cup the Isotopes just won! But disaster struck!
Someone stole the cup! It is very important we get the cup back. We have to find out who did it.
The thief left several clues at the scene of the crime:
Some pollen
Some hair
Some sand
Some fibres

He/she wasnt a very good thief!



There were the six suspects. We decided we could not use real people as suspects so we chose to go
with Harry Potter villains
Delores Umbridge
When she was interviewed Delores Umbridge was wearing a red flower. She has brown straight hair; She
had sandy shoes because she played golf and her golf ball went into a sandy bunker; She was dressed
in pink clothes.
Bellatrix Lestrange

When she was interviewed: Bellatrix Lestrange was wearing a pink flower; she has brown straight
hair; she had sandy shoes because she was in the garden; she was wearing blue clothes.
Draco Malfoy
When he was interviewed Draco Malfoy was wearing a lily flower; he has blond straight hair; he had
sandy shoes because he took a short cut through the sand box in the garden; he was wearing grey clothes.
Voldemort

When he was interviewed: Voldemort was wearing a red flower; no hair; he had sandy shoes because he
went hiking along a trail in California; he was wearing grey clothes.
Lucius Malfoy

When he was interviewed: Lucius Malfoy was wearing a pink flower; he has blond straight hair; he had
sandy shoes because he went hiking along a trail in California; he was wearing black clothes
Severus Snape

When he was interviewed: Severus Snape was wearing a lily flower; he has straight grey hair; he
had sandy shoes because he played golf and his golf ball went into a sandy bunker; he was wearing
black clothes



Mostly the clues fit whatever materials we had, such as what three flowers with lots of pollen were
available to us that day. Two suspects for each clue.


We didnt want one clue to give the suspect away. It had to be two clues. Each group took on one
type of material. On the stub there were three possibles and the actual to compare. So we had a
flipchart at headquarters (the Outreach Booth) with the suspects down one side .and clues along the
top. As each of the four groups got their answer they put a tick in the right box. It worked just fine.

In Portland we had access to an X-ray analysis sem. The story was:

Someone came to the desk in the Museum and handed an envelope over to the cashier. Written on the
envelope was Put all the cash in the envelope and dont say anything. Ive got a gun!
She complied but followed him to see where he went. He took a bicycle and went through a
construction site (sand), shaped the bike along a wall (metal for X-ray analysis), over a flower bed
(pollen) which had thorny rose bushes (fibres). By that evening the police had four suspects each
with sandy shoes and bike wheels, different materials making up the bikes etc etc.

In Nashville: Someone stole a very valuable Elvis guitar and put it up for sale clandestinely. One
of the potential buyers wanted it authenticated by identifying the strings by X-ray analysis. I went
to our local music store and asked if they had any strings from 1960s. They gave me one and I bought
a new one. The composition is very different and easy to identify by X-ray analysis.

Now I need a story for Baltimore. Ive never been to Baltimore. Is there something special we can
use in a story?
Best wishes

Elaine

Dr. Elaine C. Humphrey
Advanced Microscopy Facility
Bob Wright Science Centre A015
University of Victoria, Canada
Lab: 250-853-3968
cell: 250-886-2068
website:
https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.stehm.uvic.ca&data=02%7C01%7C14nnb1%40queensu.ca%7Cac6f78509dae425740e608d538640940%7Cd61ecb3b38b142d582c4efb2838b925c%7C1%7C0%7C636476924664745889&sdata=UWIs%2Fvo0E9DK4svQBPfEGop0Hshl1QbVrv9qy9usxoE%3D&reserved=0









} Hi Annie, et al.
}
} I did a CSI type demo using light bulb filaments. The scenario was that
} there was a traffic accident in the dark and each driver claimed the
} others headlights were not on. The headlamps in both cars were broken.
} The 2nd driver claims that the 1st driver turned on his headlights after
} the bulbs were broken in order to cover his fault. The officer collected
} the light bulb filaments from the cars and you are comparing them in the
} SEM. (You can spin out the story as much as you want!)
}
} I took 2 small incandescent bulbs (15 watt if I remember), broke the
} glass on one and then applied power so that it burned out. I took the
} 2nd and broke the glass while it was running. I then mounted the 2
} filaments on an SEM sticky tab and put them in the scope.
}
} Note: be careful about breaking the glass. Do it safely! I wore
} safety glasses and leather gloves and used a pair of channel pliers to
} hold the bulb inside a mostly closed cardboard box to contain the glass
} fragments.
}
} On examination, the student will find small spherical globs on one
} filament and none on the other. Doing EDX shows that the spherical
} globs are Si and O (i.e. glass that melted). The inference is that the
} filament was hot when the glass was broken, hence the light bulb was on.
} The other bulb is just oxidized and doesn't show any melted glass.
} Hence it was not running when the glass broke and was burned out
} afterwards.
}
} They seemed to enjoy the "real life" problem solving.
}
} Cheers,
} Henk
}
}
}
}
} --------------------
}
} Hendrik O. Colijn
} Center for Electron Microscopy and AnalysiS
} The Ohio State University
} 1305 Kinnear Rd, Suite 100, Columbus, OH 43212
}
} colijn.1-at-osu.edu 614/643-3458
} cemas.osu.edu
}
} "Time is that quality of nature which keeps things from happening all at
} once." (Ray Cummings - 1922)
} Lately it doesn't seem to be working.
}
} ------ Original Message ------
} X-from: microscopy.listserver-at-gmail.com
} To: colijn.1-at-osu.edu
} Sent: 11/20/2017 8:38:57 PM
} Subject: [Microscopy] viaWWW:EDS and SEM projector for Outreach
}
} }
} }
} }
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
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From: tina-at-pbrc.hawaii.edu
Date: Fri, 1 Dec 2017 14:44:27 -0600
Subject: [Microscopy] Need help from patho/histo types

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi, Everybody-

I've been helping a wildlife disease specialist at the USGS try to figure
out what's wrong with a population of endangered crows that are dying off
at an alarming rate. If you are interested and think you can offer an
opinion, please look at the images on this server (the link is only good
for one week):

https://www.hawaii.edu/filedrop/dl/HJIGm-dWRkO-nvQxw-aiXcJ/

These photomicrographs are from the lung of a critically endangered crow
whose populations are declining probably because of pneumonia and
hepatitis of unknown origin. On histology, crows manifest mild-to-marked
non-suppurative interstitial pneumonia with varying degrees of vasculitis.
No known organisms (protozoa, bacteria, fungi, viruses) are seen either on
light or ultrastructural microscopy, and all laboratory tests for
infectious agents are so far negative. On TEM, we are consistently
seeing a similar pattern in lungs with filamentous tubular structures ca.
10-20 um diameter surrounding blood vessels. Might anyone have ideas of
what these structure could be??

Aloha from rainy and gloomy Honolulu,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************

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From: tina-at-pbrc.hawaii.edu
Date: Fri, 1 Dec 2017 15:15:04 -0600
Subject: [Microscopy] Crow Lung link

Contents Retrieved from Microscopy Listserver Archives
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Hi, not sure why the link to the images expired immediately. I'm going to
try again:

https://www.hawaii.edu/filedrop/dl/LUyGN-wWQkV-rJxza-NjhLp/

Aloha,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************

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From: tina-at-pbrc.hawaii.edu
Date: Fri, 1 Dec 2017 17:18:04 -0600
Subject: [Microscopy] Another crow link

Contents Retrieved from Microscopy Listserver Archives
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My apologies to everyone for spamming their email. More problems with
technology than usual today. Here's another try to link to the crow lung
images:

https://drive.google.com/drive/folders/1fVP73UXRPap6yospEVMNX0xk-esqUqYz?usp=sharing

Again, any opinions, musings, or speculations appreciated!

Aloha,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************

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From: microscopy.listserver-at-gmail.com
Date: Fri, 1 Dec 2017 18:44:40 -0600
Subject: [Microscopy] viaWWW:EDS and SEM projector for Outreach

Contents Retrieved from Microscopy Listserver Archives
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X-from: KEN Livi {klivi-at-jhu.edu}

Unfortunately the McCormick warehouses moved up to the north side of the city many years ago. I miss
the smells that were there by the Inner Harbor.
There is also a railroad theme in that the first railroad station (B&O) is situated just west of
Baltimore. There could be steel, coal, and slag involved.
There is the Johns Hopkins Hospital with a plethora of medical samples (blood?).
The Chesapeake Bay has lots of biota.
There is the infamous Chromium smelting talus that the Inner Harbor is partially built on (maybe you
don’t want to have samples of Hexavalent Chromium around).
Also, the Babe Ruth Museum for a baseball theme (including the Orioles).
The Poe theme can have both raven’s feather and Ravens leather. To add a twist to that John Astin
(Gomez Addams from the Addam Family) was born here and teaches at Johns Hopkins.

Then there is history of Frederick Douglas and the Underground Railroad.

Fort McHenry where the Star Spangled Banner was penned by Francis Scott Key and the flag sewn by
Mary Pickersgill in 1813 that resides in the Smithsonian. There are cloth fibers (red and blue wool
and white cotton) and gun powder themes here.

Enoch Pratt Free Library — one of the oldest libraries in the US, Peabody Library focused on music
(paper, vinyl, tape).

There’s much more but I have to do some work today.

Ken

Kenneth JT Livi, PhD
Director, Materials Characterization and Processing Center
Materials Science and Engineering
3400 N Charles Street
Johns Hopkins University
Baltimore, Maryland 21218 USA


} On Dec 1, 2017, at 7:55 AM, microscopy.listserver-at-gmail.com wrote:
}
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
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}
} X-from: Colijn, Hendrik {colijn.1-at-osu.edu}
}
}
}
} Edgar Allan Poe theme?
} The Murders in the Rue Morgue?
} Probably not "the pit and the pendulum" or "the cask of amontillado"
}
} Not sure what you could do with crab cakes! I believe that McCormick's has a large spice facility
} near the harbor front. We could smell it the last time we were there.
}
} Cheers,
} Henk
}
} Sent from my Verizon 4G LTE Smartphone
}
} ------ Original message------
} *From: *microscopy.listserver-at-gmail.com
} *Date: *Thu, Nov 30, 2017 9:12 PM
} *To: *Colijn, Hendrik;
} *Cc: *
} *Subject:*[Microscopy] Re: viaWWW:EDS and SEM projector for Outreach
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
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} X-from: Elaine Humphrey {ech-at-uvic.ca}
} To: annie.muske-dukes-driggs-at-lonza.com {annie.muske-dukes-driggs-at-lonza.com}
}
} Hi Annie and Henk
} Thank you so much for this question. I am always in need of good ideas.
}
} We usually have a Solve the Mystery in the Family Affair, which is changing its name this year to
} Microscopic Explorations, for the delegates families and friends at the M&M conference.
}
} The pattern has developed whereby in a session on the Wednesday afternoon, it typically starts with
} an introduction and then a lot of hands on activities using loupes and light microscopes adapted
} from the GEMS Microscopical Explorations activities. At least three of the stations have a “solve
} the mystery.” At the next meeting in Baltimore we are developing one step further and we hope to
} have Fold scopes and micro meteorites.
}
} Then the participants go to the vendor hall where we usually have access to four sems to “Solve the
} Mystery”.
}
} I have tried to correlate the mystery with the place and change it each year. For instance the CSI
} Albuquerque story was: The Governor of New Mexico is coming to town. He is a baseball fan and his
} favorite team is the Albuquerque Isotopes.
} He was going to have his photograph taken with the cup the Isotopes just won! But disaster struck!
} Someone stole the cup! It is very important we get the cup back. We have to find out who did it.
} The thief left several clues at the scene of the crime:
} Some pollen
} Some hair
} Some sand
} Some fibres
}
} He/she wasn’t a very good thief!
}
}
}
} There were the six suspects. We decided we could not use real people as suspects so we chose to go
} with Harry Potter villains
} Delores Umbridge
} When she was interviewed Delores Umbridge was wearing a red flower. She has brown straight hair; She
} had sandy shoes because she played golf and her golf ball went into a sandy bunker; She was dressed
} in pink clothes.
} Bellatrix Lestrange
}
} When she was interviewed: Bellatrix Lestrange was wearing a pink flower; she has brown straight
} hair; she had sandy shoes because she was in the garden; she was wearing blue clothes.
} Draco Malfoy
} When he was interviewed Draco Malfoy was wearing a lily flower; he has blond straight hair; he had
} sandy shoes because he took a short cut through the sand box in the garden; he was wearing grey clothes.
} Voldemort
}
} When he was interviewed: Voldemort was wearing a red flower; no hair; he had sandy shoes because he
} went hiking along a trail in California; he was wearing grey clothes.
} Lucius Malfoy
}
} When he was interviewed: Lucius Malfoy was wearing a pink flower; he has blond straight hair; he had
} sandy shoes because he went hiking along a trail in California; he was wearing black clothes
} Severus Snape
}
} When he was interviewed: Severus Snape was wearing a lily flower; he has straight grey hair; he
} had sandy shoes because he played golf and his golf ball went into a sandy bunker; he was wearing
} black clothes
}
}
}
} Mostly the clues fit whatever materials we had, such as what three flowers with lots of pollen were
} available to us that day. Two suspects for each clue.
}
}
} We didn’t want one clue to give the suspect away. It had to be two clues. Each group took on one
} type of material. On the stub there were three possibles and the actual to compare. So we had a
} flipchart at headquarters (the Outreach Booth) with the suspects down one side .and clues along the
} top. As each of the four groups got their answer they put a tick in the right box. It worked just fine.
}
} In Portland we had access to an X-ray analysis sem. The story was:
}
} Someone came to the desk in the Museum and handed an envelope over to the cashier. Written on the
} envelope was “ Put all the cash in the envelope and don’t say anything. I’ve got a gun!”
} She complied but followed him to see where he went. He took a bicycle and went through a
} construction site (sand), shaped the bike along a wall (metal for X-ray analysis), over a flower bed
} (pollen) which had thorny rose bushes (fibres). By that evening the police had four suspects each
} with sandy shoes and bike wheels, different materials making up the bikes etc etc.
}
} In Nashville: Someone stole a very valuable Elvis guitar and put it up for sale clandestinely. One
} of the potential buyers wanted it authenticated by identifying the strings by X-ray analysis. I went
} to our local music store and asked if they had any strings from 1960s. They gave me one and I bought
} a new one. The composition is very different and easy to identify by X-ray analysis.
}
} Now I need a story for Baltimore. I’ve never been to Baltimore. Is there something special we can
} use in a story?
} Best wishes
}
} Elaine
}
} Dr. Elaine C. Humphrey
} Advanced Microscopy Facility
} Bob Wright Science Centre A015
} University of Victoria, Canada
} Lab: 250-853-3968
} cell: 250-886-2068
} website: http://www.stehm.uvic.ca
}
}
}
}
}
}
}
}
}
} } Hi Annie, et al.
} }
} } I did a CSI type demo using light bulb filaments. The scenario was that
} } there was a traffic accident in the dark and each driver claimed the
} } others headlights were not on. The headlamps in both cars were broken.
} } The 2nd driver claims that the 1st driver turned on his headlights after
} } the bulbs were broken in order to cover his fault. The officer collected
} } the light bulb filaments from the cars and you are comparing them in the
} } SEM. (You can spin out the story as much as you want!)
} }
} } I took 2 small incandescent bulbs (15 watt if I remember), broke the
} } glass on one and then applied power so that it burned out. I took the
} } 2nd and broke the glass while it was running. I then mounted the 2
} } filaments on an SEM sticky tab and put them in the scope.
} }
} } Note: be careful about breaking the glass. Do it safely! I wore
} } safety glasses and leather gloves and used a pair of channel pliers to
} } hold the bulb inside a mostly closed cardboard box to contain the glass
} } fragments.
} }
} } On examination, the student will find small spherical globs on one
} } filament and none on the other. Doing EDX shows that the spherical
} } globs are Si and O (i.e. glass that melted). The inference is that the
} } filament was hot when the glass was broken, hence the light bulb was on.
} } The other bulb is just oxidized and doesn't show any melted glass.
} } Hence it was not running when the glass broke and was burned out
} } afterwards.
} }
} } They seemed to enjoy the "real life" problem solving.
} }
} } Cheers,
} } Henk
} }
} }
} }
} }
} } --------------------
} }
} } Hendrik O. Colijn
} } Center for Electron Microscopy and AnalysiS
} } The Ohio State University
} } 1305 Kinnear Rd, Suite 100, Columbus, OH 43212
} }
} } colijn.1-at-osu.edu 614/643-3458
} } cemas.osu.edu
} }
} } "Time is that quality of nature which keeps things from happening all at
} } once." (Ray Cummings - 1922)
} } Lately it doesn't seem to be working.
} }
} } ------ Original Message ------
} } X-from: microscopy.listserver-at-gmail.com
} } To: colijn.1-at-osu.edu
} } Sent: 11/20/2017 8:38:57 PM
} } Subject: [Microscopy] viaWWW:EDS and SEM projector for Outreach
} }
} } }
} } }
} } }
} } } ----------------------------------------------------------------------------
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} } } X-from: Greg Baty {gbaty-at-pdx.edu}
} } }
} } }
} } } Annie,
} } }
} } } I usually deal with kids in the 12 - 18 YO range for outreach.ย In the
} } } past we offered a week long
} } } half day experience in the lab.ย In my experience it is critical to
} } } find samples that kids can
} } } relate to.
} } }
} } } Insects can be a good for imaging.ย My favorites are honey bees, house
} } } fly's and fruit fly's.ย Kids
} } } really like the structure of the eye.
} } }
} } } Epsom salt has a nice structure and is useful for EDX; however, it can
} } } take a long time to get
} } } vacuum since it is usually hydrated.ย Best to only use a few crystals
} } } or prepump.
} } }
} } } Geological samples.ย I prefer a thin section to reduce pump time, but
} } } bulk sections work too.ย If
} } } you need a thin section I suspect Spectrum Petrographics could help. I
} } } have no financial connection
} } } to them, but I know several of our geology Professors have Spectrum do
} } } their mounts.
} } }
} } } I had one student bring hair and feathers from the farm she lives on.ย
} } } There is a significant
} } } difference between animals.
} } }
} } } House hold dust can be a good sample.ย Just pick a spot like a window
} } } sill and don't clean it for a
} } } week or two.ย Collect the sample with C tape on a stub.ย I have seen
} } } pollen, insect parts and
} } } silicate minerals.
} } }
} } } Greg
} } }
} } } On Fri, Nov 17, 2017 at 3:49 PM, {microscopy.listserver-at-gmail.com
} } } {mailto:microscopy.listserver-at-gmail.com} } wrote:
} } }
} } }
} } }
} } }
} } }
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} } } X-from: annie.muske-dukes-driggs-at-lonza.com
} } } {mailto:annie.muske-dukes-driggs-at-lonza.com}
} } }
} } } This Question/Comment was submitted to the Microscopy Listserver
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} } }
} } } Email: annie.muske-dukes-driggs-at-lonza.com
} } } {mailto:annie.muske-dukes-driggs-at-lonza.com} Name:
} } } Annie Muske-Dukes
} } }
} } } Organization: Bend Research
} } }
} } } Title-Subject: [Filtered] EDS and SEM projector for Outreach
} } }
} } } Message: Hi everyone,
} } }
} } } My company runs a community outreach program for local elementary
} } } school
} } } students (8-10 years old). They tour our facility and see quick
} } } demonstrations/lectures on a variety of topics.
} } } We have had an SEM demo for years and I would like to incorporate
} } } our
} } } EDS but I'm having trouble coming up with samples that kids that
} } } age
} } } understand, especially since I only have 15 minutes per group and
} } } we try
} } } to go through at least four samples.
} } }
} } } I have generated a map of different elements in a vitamin tablet
} } } which
} } } went over well and gives them a good understanding of what the
} } } system is
} } } doing. Any recommendations for samples that generate interesting
} } } maps or
} } } have unexpected elements? I'll be doing the prep/high quality maps
} } } ahead
} } } of time so that doesn't need to be done in the 15 minutes.
} } }
} } } I'd also like to get a wireless projector to use with our Hitachi
} } } SU3500
} } } to project a live image of the screen onto the wall. Our service
} } } rep
} } } said some models can cause driver issues so if anyone here has a
} } } model
} } } that works please let me know. We need to stay under US$500, but
} } } under
} } } $300 would be ideal.
} } }
} } } Thanks,
} } } Annie (I am a list subscriber but our required corporate
} } } attachments
} } } prevent me from sending the usual way)
} } }
} } } ย Login Host: 64.47.109.63
} } } ย Listserver Email Form V - 20120416
} } }
} } } ---------------------------------------------------------------------------
} } }
} } }
} } }
} } } ==============================Original
} } } Headers==============================
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} } }
} } }
} } } --
} } } Greg Baty
} } } Center for Electron Microscopy and Nanofabrication
} } } Portland State University
} } } 503-725-2867
} } } www.pdx.edu/cemn {http://www.pdx.edu/cemn} {http://www.pdx.edu/cemn}
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} } } ==============================Original
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From: microscopy.listserver-at-gmail.com
Date: Fri, 1 Dec 2017 18:45:35 -0600
Subject: [Microscopy] viaWWW: LEO 1450 VP SEM users

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Email: kevin-at-semionco.com Name: Kevin Filter

Organization: Semion llc

Title-Subject: [Filtered] LEO 1450 VP SEM users

Message: Hello Listserver community. I would like to connect with other users of LEO 1450 VP SEMs
and similar LEOs and Tungsten Zeiss instruments. I need some help learning the quirks of this
machine and maybe getting some hints to optimize performance. If you use a LEO 1450 VP or similar
instrument and are willing to take some questions please contact me. Thanks, Kevin

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From: microscopy.listserver-at-gmail.com
Date: Sun, 3 Dec 2017 09:54:17 -0600
Subject: [Microscopy] Fwd: viaWWW:coating for Carbon evaporator bell jar

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Email: duleyml-at-miamioh.edu Name: matthew duley

Organization: Center for Advanced Microscopy & Imaging / Miami University

Title-Subject: [Filtered] coating for Carbon evaporator bell jar

Message: Afternoon all,

In a previous post I asked what everyone was doing for coating the bell jar on your carbon
evaporator, now that Bell Bright wasn’t going to be available. It seemed the general consensus was
to use dish soap as a replacement. Well, I’ve exhausted my hoard and like a dummy I didn’t ask for
how you did that. So the obvious questions are what are you using instead of Bell Bright and how do
you apply it?

TIA

Matthew L. Duley

Microscopy Specialist
Center for Advanced Microscopy & Imaging
9B Upham Hall
Miami University
Oxford, Ohio 45056
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X-from: John Nailon {jvnailon-at-gmail.com}


G'day Matthew,

It is very simple and inexpensive, clean your bell jar, electrodes, and insulators as best you can
then wipe the inside with a piece of paper towel that has had 5-10ml of dishwashing detergent
applied to it. Wipe the whole of the glass, metal and insulator surfaces so that there is a very
thin smear of detergent applied. Pump down the bell jar to dry the system out. The system is ready
to use!
Next time you need to clean the system all you need do is wash all surfaces with warm water, dry
them off with paper towel, then apply the detergent again.

Regards
John V Nailon
Retired Electron Microscopist

John V Nailon
Mob: 0423 020 680
Email: jvnailon-at-gmail.com {mailto:jvnailon-at-gmail.com}


On Sat, Dec 2, 2017 at 12:11 PM, {microscopy.listserver-at-gmail.com
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Email: duleyml-at-miamioh.edu {mailto:duleyml-at-miamioh.edu} Name: matthew duley

Organization: Center for Advanced Microscopy & Imaging / Miami University

Title-Subject: [Filtered] coating for Carbon evaporator bell jar

Message: Afternoon all,

In a previous post I asked what everyone was doing for coating the bell jar on your carbon
evaporator, now that Bell Bright wasn’t going to be available.  It seemed the general consensus was
to use dish soap as a replacement. Well, I’ve exhausted my hoard and like a dummy I didn’t ask for
how you did that.  So the obvious questions are what are you using instead of Bell Bright and
how do
you apply it?

TIA

Matthew L. Duley

Microscopy Specialist
Center for Advanced Microscopy & Imaging
9B Upham Hall
Miami University
Oxford, Ohio  45056
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From: microscopy.listserver-at-gmail.com
Date: Mon, 4 Dec 2017 09:54:26 -0600
Subject: [Microscopy] viaWWW:EDS and SEM projector for Outreach

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Ambrose, Wallace W {Wallace_Ambrose-at-unc.edu}

Listers, Baltimore is a great city, some faults notwithstanding. It has a wealth of old buildings
and structures that have built up many layers of paint. I collected a nice thick paint sample (many
layers) from a lamp post near Fell's Point to compare with some samples I collected on our campus.
This was used in the SEM/EDS section of a graduate instrumentation class. I called it " The Doorway,
The Downspout and the Lamp Post". It was a great introduction to examining various colors and
constituents in paint layers, as well as to restoration processes. Lead paints could be dated as
pre late 1950s. Wallace Ambrose
Applied Physical Sciences
University or North Carolina

-----Original Message-----
X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Friday,
December 01, 2017 8:16 PM
To: Ambrose, Wallace W {Wallace_Ambrose-at-unc.edu}

X-from: KEN Livi {klivi-at-jhu.edu}

Unfortunately the McCormick warehouses moved up to the north side of the city many years ago. I miss
the smells that were there by the Inner Harbor.
There is also a railroad theme in that the first railroad station (B&O) is situated just west of
Baltimore. There could be steel, coal, and slag involved.
There is the Johns Hopkins Hospital with a plethora of medical samples (blood?).
The Chesapeake Bay has lots of biota.
There is the infamous Chromium smelting talus that the Inner Harbor is partially built on (maybe you
don’t want to have samples of Hexavalent Chromium around).
Also, the Babe Ruth Museum for a baseball theme (including the Orioles).
The Poe theme can have both raven’s feather and Ravens leather. To add a twist to that John Astin
(Gomez Addams from the Addam Family) was born here and teaches at Johns Hopkins.

Then there is history of Frederick Douglas and the Underground Railroad.

Fort McHenry where the Star Spangled Banner was penned by Francis Scott Key and the flag sewn by
Mary Pickersgill in 1813 that resides in the Smithsonian. There are cloth fibers (red and blue wool
and white cotton) and gun powder themes here.

Enoch Pratt Free Library — one of the oldest libraries in the US, Peabody Library focused on music
(paper, vinyl, tape).

There’s much more but I have to do some work today.

Ken

Kenneth JT Livi, PhD
Director, Materials Characterization and Processing Center
Materials Science and Engineering
3400 N Charles Street
Johns Hopkins University
Baltimore, Maryland 21218 USA


} On Dec 1, 2017, at 7:55 AM, microscopy.listserver-at-gmail.com wrote:
}
}
}
}
}
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} X-from: Colijn, Hendrik {colijn.1-at-osu.edu}
}
}
}
} Edgar Allan Poe theme?
} The Murders in the Rue Morgue?
} Probably not "the pit and the pendulum" or "the cask of amontillado"
}
} Not sure what you could do with crab cakes! I believe that McCormick's has a large spice facility
} near the harbor front. We could smell it the last time we were there.
}
} Cheers,
} Henk
}
} Sent from my Verizon 4G LTE Smartphone
}
} ------ Original message------
} *From: *microscopy.listserver-at-gmail.com
} *Date: *Thu, Nov 30, 2017 9:12 PM
} *To: *Colijn, Hendrik;
} *Cc: *
} *Subject:*[Microscopy] Re: viaWWW:EDS and SEM projector for Outreach
}
}
}
}
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} X-from: Elaine Humphrey {ech-at-uvic.ca}
} To: annie.muske-dukes-driggs-at-lonza.com {annie.muske-dukes-driggs-at-lonza.com}
}
} Hi Annie and Henk
} Thank you so much for this question. I am always in need of good ideas.
}
} We usually have a Solve the Mystery in the Family Affair, which is changing its name this year to
} Microscopic Explorations, for the delegates families and friends at the M&M conference.
}
} The pattern has developed whereby in a session on the Wednesday afternoon, it typically starts with
} an introduction and then a lot of hands on activities using loupes and light microscopes adapted
} from the GEMS Microscopical Explorations activities. At least three of the stations have a “solve
} the mystery.” At the next meeting in Baltimore we are developing one step further and we hope to
} have Fold scopes and micro meteorites.
}
} Then the participants go to the vendor hall where we usually have access to four sems to “Solve the
} Mystery”.
}
} I have tried to correlate the mystery with the place and change it each year. For instance the CSI
} Albuquerque story was: The Governor of New Mexico is coming to town. He is a baseball fan and his
} favorite team is the Albuquerque Isotopes.
} He was going to have his photograph taken with the cup the Isotopes just won! But disaster struck!
} Someone stole the cup! It is very important we get the cup back. We have to find out who did it.
} The thief left several clues at the scene of the crime:
} Some pollen
} Some hair
} Some sand
} Some fibres
}
} He/she wasn’t a very good thief!
}
}
}
} There were the six suspects. We decided we could not use real people as suspects so we chose to go
} with Harry Potter villains
} Delores Umbridge
} When she was interviewed Delores Umbridge was wearing a red flower. She has brown straight hair; She
} had sandy shoes because she played golf and her golf ball went into a sandy bunker; She was dressed
} in pink clothes.
} Bellatrix Lestrange
}
} When she was interviewed: Bellatrix Lestrange was wearing a pink flower; she has brown straight
} hair; she had sandy shoes because she was in the garden; she was wearing blue clothes.
} Draco Malfoy
} When he was interviewed Draco Malfoy was wearing a lily flower; he has blond straight hair; he had
} sandy shoes because he took a short cut through the sand box in the garden; he was wearing grey clothes.
} Voldemort
}
} When he was interviewed: Voldemort was wearing a red flower; no hair; he had sandy shoes because he
} went hiking along a trail in California; he was wearing grey clothes.
} Lucius Malfoy
}
} When he was interviewed: Lucius Malfoy was wearing a pink flower; he has blond straight hair; he had
} sandy shoes because he went hiking along a trail in California; he was wearing black clothes
} Severus Snape
}
} When he was interviewed: Severus Snape was wearing a lily flower; he has straight grey hair; he
} had sandy shoes because he played golf and his golf ball went into a sandy bunker; he was wearing
} black clothes
}
}
}
} Mostly the clues fit whatever materials we had, such as what three flowers with lots of pollen were
} available to us that day. Two suspects for each clue.
}
}
} We didn’t want one clue to give the suspect away. It had to be two clues. Each group took on one
} type of material. On the stub there were three possibles and the actual to compare. So we had a
} flipchart at headquarters (the Outreach Booth) with the suspects down one side .and clues along the
} top. As each of the four groups got their answer they put a tick in the right box. It worked just fine.
}
} In Portland we had access to an X-ray analysis sem. The story was:
}
} Someone came to the desk in the Museum and handed an envelope over to the cashier. Written on the
} envelope was “ Put all the cash in the envelope and don’t say anything. I’ve got a gun!”
} She complied but followed him to see where he went. He took a bicycle and went through a
} construction site (sand), shaped the bike along a wall (metal for X-ray analysis), over a flower bed
} (pollen) which had thorny rose bushes (fibres). By that evening the police had four suspects each
} with sandy shoes and bike wheels, different materials making up the bikes etc etc.
}
} In Nashville: Someone stole a very valuable Elvis guitar and put it up for sale clandestinely. One
} of the potential buyers wanted it authenticated by identifying the strings by X-ray analysis. I went
} to our local music store and asked if they had any strings from 1960s. They gave me one and I bought
} a new one. The composition is very different and easy to identify by X-ray analysis.
}
} Now I need a story for Baltimore. I’ve never been to Baltimore. Is there something special we can
} use in a story?
} Best wishes
}
} Elaine
}
} Dr. Elaine C. Humphrey
} Advanced Microscopy Facility
} Bob Wright Science Centre A015
} University of Victoria, Canada
} Lab: 250-853-3968
} cell: 250-886-2068
} website: http://www.stehm.uvic.ca
}
}
}
}
}
}
}
}
}
} } Hi Annie, et al.
} }
} } I did a CSI type demo using light bulb filaments. The scenario was that
} } there was a traffic accident in the dark and each driver claimed the
} } others headlights were not on. The headlamps in both cars were broken.
} } The 2nd driver claims that the 1st driver turned on his headlights after
} } the bulbs were broken in order to cover his fault. The officer collected
} } the light bulb filaments from the cars and you are comparing them in the
} } SEM. (You can spin out the story as much as you want!)
} }
} } I took 2 small incandescent bulbs (15 watt if I remember), broke the
} } glass on one and then applied power so that it burned out. I took the
} } 2nd and broke the glass while it was running. I then mounted the 2
} } filaments on an SEM sticky tab and put them in the scope.
} }
} } Note: be careful about breaking the glass. Do it safely! I wore
} } safety glasses and leather gloves and used a pair of channel pliers to
} } hold the bulb inside a mostly closed cardboard box to contain the glass
} } fragments.
} }
} } On examination, the student will find small spherical globs on one
} } filament and none on the other. Doing EDX shows that the spherical
} } globs are Si and O (i.e. glass that melted). The inference is that the
} } filament was hot when the glass was broken, hence the light bulb was on.
} } The other bulb is just oxidized and doesn't show any melted glass.
} } Hence it was not running when the glass broke and was burned out
} } afterwards.
} }
} } They seemed to enjoy the "real life" problem solving.
} }
} } Cheers,
} } Henk
} }
} }
} }
} }
} } --------------------
} }
} } Hendrik O. Colijn
} } Center for Electron Microscopy and AnalysiS
} } The Ohio State University
} } 1305 Kinnear Rd, Suite 100, Columbus, OH 43212
} }
} } colijn.1-at-osu.edu 614/643-3458
} } cemas.osu.edu
} }
} } "Time is that quality of nature which keeps things from happening all at
} } once." (Ray Cummings - 1922)
} } Lately it doesn't seem to be working.
} }
} } ------ Original Message ------
} } X-from: microscopy.listserver-at-gmail.com
} } To: colijn.1-at-osu.edu
} } Sent: 11/20/2017 8:38:57 PM
} } Subject: [Microscopy] viaWWW:EDS and SEM projector for Outreach
} }
} } }
} } }
} } }
} } } ----------------------------------------------------------------------------
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} } }
} } } X-from: Greg Baty {gbaty-at-pdx.edu}
} } }
} } }
} } } Annie,
} } }
} } } I usually deal with kids in the 12 - 18 YO range for outreach.ย In the
} } } past we offered a week long
} } } half day experience in the lab.ย In my experience it is critical to
} } } find samples that kids can
} } } relate to.
} } }
} } } Insects can be a good for imaging.ย My favorites are honey bees, house
} } } fly's and fruit fly's.ย Kids
} } } really like the structure of the eye.
} } }
} } } Epsom salt has a nice structure and is useful for EDX; however, it can
} } } take a long time to get
} } } vacuum since it is usually hydrated.ย Best to only use a few crystals
} } } or prepump.
} } }
} } } Geological samples.ย I prefer a thin section to reduce pump time, but
} } } bulk sections work too.ย If
} } } you need a thin section I suspect Spectrum Petrographics could help. I
} } } have no financial connection
} } } to them, but I know several of our geology Professors have Spectrum do
} } } their mounts.
} } }
} } } I had one student bring hair and feathers from the farm she lives on.ย
} } } There is a significant
} } } difference between animals.
} } }
} } } House hold dust can be a good sample.ย Just pick a spot like a window
} } } sill and don't clean it for a
} } } week or two.ย Collect the sample with C tape on a stub.ย I have seen
} } } pollen, insect parts and
} } } silicate minerals.
} } }
} } } Greg
} } }
} } } On Fri, Nov 17, 2017 at 3:49 PM, {microscopy.listserver-at-gmail.com
} } } {mailto:microscopy.listserver-at-gmail.com} } wrote:
} } }
} } }
} } }
} } }
} } }
} } } ----------------------------------------------------------------------------
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} } }
} } } X-from: annie.muske-dukes-driggs-at-lonza.com
} } } {mailto:annie.muske-dukes-driggs-at-lonza.com}
} } }
} } } This Question/Comment was submitted to the Microscopy Listserver
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} } } replying pleaseย copyย both annie.muske-dukes-driggs-at-lonza.com
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} } } ---------------------------------------------------------------------------
} } }
} } } Email: annie.muske-dukes-driggs-at-lonza.com
} } } {mailto:annie.muske-dukes-driggs-at-lonza.com} Name:
} } } Annie Muske-Dukes
} } }
} } } Organization: Bend Research
} } }
} } } Title-Subject: [Filtered] EDS and SEM projector for Outreach
} } }
} } } Message: Hi everyone,
} } }
} } } My company runs a community outreach program for local elementary
} } } school
} } } students (8-10 years old). They tour our facility and see quick
} } } demonstrations/lectures on a variety of topics.
} } } We have had an SEM demo for years and I would like to incorporate
} } } our
} } } EDS but I'm having trouble coming up with samples that kids that
} } } age
} } } understand, especially since I only have 15 minutes per group and
} } } we try
} } } to go through at least four samples.
} } }
} } } I have generated a map of different elements in a vitamin tablet
} } } which
} } } went over well and gives them a good understanding of what the
} } } system is
} } } doing. Any recommendations for samples that generate interesting
} } } maps or
} } } have unexpected elements? I'll be doing the prep/high quality maps
} } } ahead
} } } of time so that doesn't need to be done in the 15 minutes.
} } }
} } } I'd also like to get a wireless projector to use with our Hitachi
} } } SU3500
} } } to project a live image of the screen onto the wall. Our service
} } } rep
} } } said some models can cause driver issues so if anyone here has a
} } } model
} } } that works please let me know. We need to stay under US$500, but
} } } under
} } } $300 would be ideal.
} } }
} } } Thanks,
} } } Annie (I am a list subscriber but our required corporate
} } } attachments
} } } prevent me from sending the usual way)
} } }
} } } ย Login Host: 64.47.109.63
} } } ย Listserver Email Form V - 20120416
} } }
} } } ---------------------------------------------------------------------------
} } }
} } }
} } }
} } } ==============================Original
} } } Headers==============================
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} } } 13, 53 -- Subject: viaWWW:EDS and SEM projector for Outreach
} } } 13, 53 -- References: {201711162229.vAGMTWp6026431-at-microscopy.com
} } } {mailto:201711162229.vAGMTWp6026431-at-microscopy.com} }
} } } 13, 53 -- To: MicroscopyListServer-Forward
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} } } {201711162229.vAGMTWp6026431-at-microscopy.com
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} } } 13, 53 -- Message-ID:
} } } {4230175d-0ecf-bad3-c4f0-4f12f99a8f95-at-gmail.com
} } } {mailto:4230175d-0ecf-bad3-c4f0-4f12f99a8f95-at-gmail.com} }
} } } 13, 53 -- Date: Sat, 18 Nov 2017 07:15:22 +0800
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} } } ==============================End of -
} } } Headers==============================
} } }
} } }
} } }
} } }
} } } --
} } } Greg Baty
} } } Center for Electron Microscopy and Nanofabrication
} } } Portland State University
} } } 503-725-2867
} } } www.pdx.edu/cemn {http://www.pdx.edu/cemn} {http://www.pdx.edu/cemn}
} } }
} } } ==============================Original
} } } Headers==============================
} } } 33, 53 -- From microscopy.listserver-at-gmail.com Mon Nov 20 19:36:44 2017
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} 34, 53 -- From microscopy.listserver-at-gmail.com Thu Nov 30 20:12:45 2017
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From: microscopy.listserver-at-gmail.com
Date: Mon, 4 Dec 2017 09:55:36 -0600
Subject: [Microscopy] viaWWW:coating for Carbon evaporator bell jar

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: JD Arnott {jd-at-laddresearch.com}

Well, we offer BellShine at

https://www.laddresearch.com/vacuum-equipment/bell-shine

that should work for you.
Call and ask for Mike Bouchard if you want to discuss it.

Thanks,


JD Arnott

President

Disclaimer: Ladd research sells this product and other products for use in
Microscopy Labs.


Join us on Facebook at https://www.facebook.com/pages/Ladd-Research

And Twitter at https://twitter.com/laddresearch

Ladd Research
83 Holly Court
Williston, VT 05495

T- 802-658-4961
F- 802-660-8859

www.laddresearch.com



-----Original Message-----
X-from: microscopy.listserver-at-gmail.com
[mailto:microscopy.listserver-at-gmail.com] Sent: Friday, December 01, 2017 7:58 PM
To: jd-at-laddresearch.com

X-from: duleyml-at-miamioh.edu

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Email: duleyml-at-miamioh.edu Name: matthew duley

Organization: Center for Advanced Microscopy & Imaging / Miami University

Title-Subject: [Filtered] coating for Carbon evaporator bell jar

Message: Afternoon all,

In a previous post I asked what everyone was doing for coating the bell jar
on your carbon evaporator, now that Bell Bright wasn’t going to be
available. It seemed the general consensus was to use dish soap as a
replacement. Well, I’ve exhausted my hoard and like a dummy I didn’t ask
for how you did that. So the obvious questions are what are you using
instead of Bell Bright and how do you apply it?

TIA

Matthew L. Duley

Microscopy Specialist
Center for Advanced Microscopy & Imaging
9B Upham Hall
Miami University
Oxford, Ohio 45056
513.529.4164
FAX 513.529.4243
Duleyml-at-muohio.edu

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From: microscopy.listserver-at-gmail.com
Date: Mon, 4 Dec 2017 09:56:21 -0600
Subject: [Microscopy] Fwd: Re: Fwd: viaWWW:coating for Carbon evaporator bell

Contents Retrieved from Microscopy Listserver Archives
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X-from: Sylvain Poudrette {spoudrette-at-videotron.ca}


Hi

I have a coater i'm refurbishing and wondering what this detergent coating is for ?

Sylvain Poudrette

Le 2017-12-03 11:45 AM, microscopy.listserver-at-gmail.com a crit :

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} X-from: John Nailon {jvnailon-at-gmail.com}
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}
} G'day Matthew,
}
} It is very simple and inexpensive, clean your bell jar, electrodes, and insulators as best you can
} then wipe the inside with a piece of paper towel that has had 5-10ml of dishwashing detergent
} applied to it. Wipe the whole of the glass, metal and insulator surfaces so that there is a very
} thin smear of detergent applied. Pump down the bell jar to dry the system out. The system is ready
} to use!
} Next time you need to clean the system all you need do is wash all surfaces with warm water, dry
} them off with paper towel, then apply the detergent again.
}
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} John V Nailon
} Retired Electron Microscopist
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} Email: duleyml-at-miamioh.edu {mailto:duleyml-at-miamioh.edu} Name: matthew duley
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} Organization: Center for Advanced Microscopy & Imaging / Miami University
}
} Title-Subject: [Filtered] coating for Carbon evaporator bell jar
}
} Message: Afternoon all,
}
} In a previous post I asked what everyone was doing for coating the bell jar on your carbon
} evaporator, now that Bell Bright wasnt going to be available. It seemed the general consensus was
} to use dish soap as a replacement. Well, Ive exhausted my hoard and like a dummy I didnt ask for
} how you did that. So the obvious questions are what are you using instead of Bell Bright and
} how do
} you apply it?
}
} TIA
}
} Matthew L. Duley
}
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From: microscopy.listserver-at-gmail.com
Date: Mon, 4 Dec 2017 10:05:20 -0600
Subject: [Microscopy] viaWWW: Electron Microscopist Position Available at The Rockefeller

Contents Retrieved from Microscopy Listserver Archives
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Email: kuryu-at-rockefeller.edu Name: Kunihiro Uryu

Organization: The Rockefeller University

Title-Subject: [Filtered] Electron Microscopist Position Available at The Rockefeller University

Message: Dear list,

The Rockefeller University, a premier biomedical research institution, seeks a Research
Support-at-Associate or Specialist to join our Electron Microscopy Resource Center (EMRC).

The EMRC provides state-of-the-art electron microscopy support for analysis of a wide variety of
biological samples, including viruses, bacteria, insects, animal tissue as well as cultured cells
and isolated cellular components for structural analyses or immuno-electron microscopy. The EMRC is
equipped with three transmission electron microscopes, a conventional and a serial block-face
imaging scanning electron microscope, and a high-pressure freezing and a freeze-substitution unit.
(http://www.rockefeller.edu/emrc/)
The Research Support Associate/Specialist will participate in all of the EMRCfs daily operations,
including maintenance, upkeep and use of the electron microscopes and associated equipment, ordering
supplies, interacting with vendors, and administrative support for office duties, including center
billing. The position also entails specimen preparation, including negative staining, ultrathin
sectioning, and immunolabeling, operation of the microscopes and associated equipment, training
users, as well as consulting scientists on the design of experiments, data processing/analysis,
interpretation of results, and informing users on the latest methodology through familiarity with
relevant literature.

The successful candidate will have an M.S./Ph.D. degree or equivalent background in biology,
bioengineering or a related field and must have a minimum of 5 years of hands-on experience in
electron microscopy. A strong background in computation would be a plus. Must have strong
communication skills, and the ability to work collaboratively in a team as well as independently on
a wide variety of research projects. Must be detail-oriented, focused, and highly motivated.

We offer a competitive salary, comprehensive benefits, and a collegial work environment. To apply to
this job, click the following URL, click on 'staff opportunitiesf and enter keyword eIRC20449f or
eElectron Microscopistf: http://www.rockefeller.edu/hr/career.php

The Rockefeller University is an Affirmative Action/Equal Employment Opportunity/VEVRAA employer.


Regards,

Hiro
------

Kunihiro Uryu, Ph.D.,
Research Assistant Professor
Director of Electron Microscopy Resource Center (EMRC)
RRB Rm120
The Rockefeller University
1230 York Ave., Box 230
New York, NY 10065

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From: microscopy.listserver-at-gmail.com
Date: Tue, 5 Dec 2017 12:13:22 -0600
Subject: [Microscopy] viaWWW:

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Michael Delannoy {mdelann1-at-jhmi.edu}

Matthew,

I have the denton floor model carbon evaporator and I use a very small dab
of metal polish on a paper towel, within a small area of the jar. I
immediately chase with 100% ethanolthen move to another small region o f
the jar. I have never had vac issues cleaning the bell jar like this.

Good luck,
Michael Delannoy

-----Original Message-----
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[mailto:microscopy.listserver-at-gmail.com] Sent: Friday, December 01, 2017 7:58 PM
To: delannoy-at-jhmi.edu

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Email: duleyml-at-miamioh.edu Name: matthew duley

Organization: Center for Advanced Microscopy & Imaging / Miami University

Title-Subject: [Filtered] coating for Carbon evaporator bell jar

Message: Afternoon all,

In a previous post I asked what everyone was doing for coating the bell jar
on your carbon evaporator, now that Bell Bright wasnBt going to be
available. It seemed the general consensus was to use dish soap as a
replacement. Well, IBve exhausted my hoard and like a dummy I didnBt ask
for how you did that. So the obvious questions are what are you using
instead of Bell Bright and how do you apply it?

TIA

Matthew L. Duley

Microscopy Specialist
Center for Advanced Microscopy & Imaging
9B Upham Hall
Miami University
Oxford, Ohio 45056
513.529.4164
FAX 513.529.4243
Duleyml-at-muohio.edu

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Email: pveril-at-uth.gr Name: Panagiotis Berillis

Organization: University of Thessaly

Title-Subject: [Filtered] CM10 ODP water message

Message: Hi all
Today I tried to switch on our Philips CM10 TEM. After an hour the message "ODP water" appeared on
the monitor. Reading the manual I figured out that the message was about high water temperature (} 60
oC)on the ODP cooling system. There is the S30 switch that checks that temperature. Our water
cooling system seems to work fine (temperature about 15oC). Any ideas how to fix the problem?

Panagiotis

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From: microscopy.listserver-at-gmail.com
Date: Tue, 5 Dec 2017 12:14:05 -0600
Subject: [Microscopy] viaWWW:Embedding Chick Brain (E7-E10) for Vibratome Sectioning

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Email: driscollg2-at-winthrop.edu Name: Garrett Driscoll

Organization: Winthrop University

Title-Subject: [Filtered] Embedding Chick Brain (E7-E10) for Vibratome Sectioning

Message: I am doing brain electroporations with an RCASB plasmid and I wanted to visualize how
efficient my electroporations were. Currently, I am embedding my heads in 3% agarose at 37 degrees
between 15-20 minutes, cutting the block fairly close to the brain, and a final embedding in 6%
agarose at 4 degrees for at least 20 minutes. The smallest sections I have been able to slice is 300
microns; however, I would like to get down to 200 microns. When I have attempted to go below 300
microns, the tissue gets destroyed, which I can clearly see that it's lacking structural support
from how I am embedding.
My question: What would be a more efficient way to embed my samples so I can section between 100-200
microns?



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From: zaluzec-at-aaem.amc.anl.gov
Date: Tue, 5 Dec 2017 12:16:36 -0600
Subject: [Microscopy] Re: viaWWW: CM10 ODP water message

Contents Retrieved from Microscopy Listserver Archives
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Panagiotis

Check the thermal sensor on the ODP, it may need replacing. This happened on my CM200.

Nestor



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} Email: pveril-at-uth.gr Name: Panagiotis Berillis
}
} Organization: University of Thessaly
}
} Title-Subject: [Filtered] CM10 ODP water message
}
} Message: Hi all
} Today I tried to switch on our Philips CM10 TEM. After an hour the message "ODP water" appeared on
} the monitor. Reading the manual I figured out that the message was about high water temperature (} 60
} oC)on the ODP cooling system. There is the S30 switch that checks that temperature. Our water
} cooling system seems to work fine (temperature about 15oC). Any ideas how to fix the problem?
}
} Panagiotis
}
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===========================================
Dr. Nestor J. Zaluzec
Argonne National Laboratory
Photon Sciences Division
9700 S. Cass Ave
Bldg 212 / A-143
Argonne, Illinois 60439 USA
Email: Zaluzec-at-aaem.amc.anl.gov


Tel: 530-NES-TORZ (530-637-8679) has Voice Mail
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Fax: 630-252-4798

iChat: Zaluzec-at-AIM.com
Skype: Zaluzec
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Senior Scientist - Argonne National Laboratory
Fellow of the Microscopy Society of America
Senior Fellow the Computational Institute - University of Chicago
E.P. Wigner Fellow - Oak Ridge National Laboratory
Past President Microscopy Society of America
Adjunct Professor of Physics - Northern Illinois University &
the University of Illinois at Chicago
Visiting Professor of Microscopy - Manchester University

===========================================
TPMLab: http://tpm.amc.anl.gov
MMSite: http://www.amc.anl.gov
===========================================

The box said ...
"This program requires Win 95/98/NT or better..."
So I bought a Mac !

===========================================
LLAP
=========================================+=====



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From: oshel1pe-at-cmich.edu
Date: Tuesday, 05December, 2017 at 13:39
Subject: [Microscopy] viaWWW:

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I had this issue with our old CM-10 for either of two reasons: either somehow the temperature sensor on the side on the ODP was out of position, or the cooling water line was clogged, and didn’t have sufficient flow.
The sensor got jogged out of position once during maintenance, so that it wasn’t making proper contact with the pump and read wrong. This shut down the diffusion pump.

The clogged cooling line was commonplace, and easily fixed by running a bottle of CLR (Calcium Lime Rust), a US brand of scale remover, through the lines. The line could also be cleaned with a liter of 3% H2O2 or 100mL of 30% H2O2.
In our case, the TEM was on a Haskris chiller, so I just added the solution to the chiller’s water tank and let the cooling water circulate for 4 hours - overnight (depending on how bad things were), then several changes of water until crud quit coming out of the cooling line.

If you’re on city water and not a cooler … hm … try removing the in-line filter from the housing and adding the H2O2 to the filter housing. This worked on an old ISI EM — except at that lab, we had a problem with biofilm, not corrosion, and used bleach.

Note: the cooling water coming in gets split into 2 lines in the CM-10, one to the ODP and one to the electronics. If there is a line clog at or after the split, the pump can overheat without affecting the electronics, and vice-versa.

Phil
-------------
Philip Oshel
Imaging Facility Director
Biology Department
1304 Biosciences
1455 Calumet Ct.
Central Michigan University
Mt. Pleasant, MI 48859
989 774-3576 office
989 774-7567 lab








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Email: pveril-at-uth.gr Name: Panagiotis Berillis

Organization: University of Thessaly

Title-Subject: [Filtered] CM10 ODP water message

Message: Hi all
Today I tried to switch on our Philips CM10 TEM. After an hour the message "ODP water" appeared on
the monitor. Reading the manual I figured out that the message was about high water temperature (} 60
oC)on the ODP cooling system. There is the S30 switch that checks that temperature. Our water
cooling system seems to work fine (temperature about 15oC). Any ideas how to fix the problem?

Panagiotis




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From: microscopy.listserver-at-gmail.com
Date: Thu, 7 Dec 2017 07:00:46 -0600
Subject: [Microscopy] viaWWW:Job Opening Univ of CA, Merced

Contents Retrieved from Microscopy Listserver Archives
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Garett,

First, are you removing the meninges before embedding the brains? They can interfere with the agarose.

Second, this may/may not help — I embedded mormyrid brains, not vertebrate. But. Try 18% (wt:vol) 100 — 175 bloom gelatin instead of agarose. Liquify the gelatin and place tissue in the gelatin in a 55-60 deg C oven for 1.5 — 2 hours. Remove from oven, blot, place in fresh gelatin & put in oven for 15 — 30 minutes, remove from oven, blot, embed in fresh gelatin but do not put back in oven. Let gelatin set completely.

Mormyrids have a ginormous cerebellum that sits over the cerebrum and causes issues with the embedding medium getting to the rest of the brain. This gelatin method worked for them, so it should work for chick brains. I hope.

Phil
-------------
Philip Oshel
Imaging Facility Director
Biology Department
1304 Biosciences
1455 Calumet Ct.
Central Michigan University
Mt. Pleasant, MI 48859
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Email: driscollg2-at-winthrop.edu Name: Garrett Driscoll

Organization: Winthrop University

Title-Subject: [Filtered] Embedding Chick Brain (E7-E10) for Vibratome Sectioning

Message: I am doing brain electroporations with an RCASB plasmid and I wanted to visualize how
efficient my electroporations were. Currently, I am embedding my heads in 3% agarose at 37 degrees
between 15-20 minutes, cutting the block fairly close to the brain, and a final embedding in 6%
agarose at 4 degrees for at least 20 minutes. The smallest sections I have been able to slice is 300
microns; however, I would like to get down to 200 microns. When I have attempted to go below 300
microns, the tissue gets destroyed, which I can clearly see that it's lacking structural support
from how I am embedding.
My question: What would be a more efficient way to embed my samples so I can section between 100-200
microns?




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20, 63 -- Subject: Re: [Microscopy] viaWWW:Embedding Chick Brain (E7-E10) for Vibratome
20, 63 -- Sectioning
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20, 63 -- Sectioning
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From shkrsugi29udma-at-gmail.com Wed Dec 6 22:56:32 2017
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Email: mtatham-at-ucmerced.edu Name: Melissa Tatham

Organization: University of CA, Merced

Title-Subject: [Filtered] Job Opening

Message: Are you able to list this open position for us?

Image and Microscopy Facility Lab Manager University of CA, Merced

https://jobs.ucmerced.edu/n/staff/position.jsf?positionId=7844

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From: microscopy.listserver-at-gmail.com
Date: Sun, 10 Dec 2017 10:06:25 -0600
Subject: [Microscopy] viaWWW:Cryo-EM Position Open at Duke

Contents Retrieved from Microscopy Listserver Archives
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Email: jhyun-at-gatan.com Name: John Hyun

Organization: Gatan

Title-Subject: [Filtered] EELS Training School April 2018, Pleasanton, CA USA

Message: EELS & EFTEM Analysis Training School - April 2018

Electron energy loss spectroscopy (EELS) is a powerful technique that provides both compositional
and chemical information from sub-nanometer areas in the sample. As a course attendee, you will
learn best practices to set up and optimize your EELS hardware and experimental protocols so you can
capture and extract the maximum amount of compositional and chemical information from your TEM
samples. Topics include:

•Fundamentals of EELS and energy-filtered imaging in TEM
•Principles of operation of EFTEM and EELS systems
•Optimization of EFTEM and EELS data acquisition
•Quantification of elemental composition
•Other information provided by EFTEM/EELS and how best to extract it
•Use of EELS signals to form maps of elemental and chemical composition
•EFTEM and STEM EELS spectrum imaging techniques
•Identification of material phases via EELS fine structure mapping
•Applications to biological and physical science specimens

Register online: http://www.gatan.com/company/events/eels-eftem-analysis-training-school-april-2018-0



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From: microscopy.listserver-at-gmail.com
Date: Sun, 10 Dec 2017 10:18:16 -0600
Subject: [Microscopy] viaWWW:Embedding Chick Brain (E7-E10) for Vibratome Sectioning

Contents Retrieved from Microscopy Listserver Archives
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X-from: Glen MacDonald {glenmac-at-u.washington.edu}

Dear Garrett,
There are some missing details. What is your fixative or is this live? Are these expressing a
fluorescent protein or are you planning on immunolabeling?
what instrument are you using to section and how are you attaching the sample to the sample holder.

I’ve found that embryonic avian brain requires slow advance speed and high amplitude, whether live
or fixed. It is also very sensitive to knife angle. How are you attaching to the specimen holder?
Not all cyanoacryates work with wet samples. Iv’e generally had the the best results orienting the
tissue with the ventral surface facing the knife. there is a tendency for the sectioning shear force
to push the brain apart when cut from the dorsal surface. As Phil mentions, surrounding membranes
can cause shear forces that displace the sample. Or they drape around the razor’s edge and get
dragged through the tissue. I’ve cut live embryonic brain down to 200 um by gluing it to the holder
and just using an agarose block to “backstop” the brain. Such a backstop can also help with any
fragile tissue that does not offer much internal strength. In another project, embryonic avian
brain was sectioned after blocking the tissue to give a flat base and gluing to the stub. Then low
melting point agarose was ladled over it and allowed to harden. Another approach for small floppy
tissues, like early embryonic brain or with spinal cord, is to cut the rounded end from a gelatin
capsule or BEEM capsule and use the capsule as a mold for embedding with agarose. If embedded with
the cap in place, remove the cap to expose the tissue. Or trim down the upper end to expose the
tissue for slicing.
Regards
Glen MacDonald
depts.washington.edu/digmicro
glenmac-at-uw.edu


} On Dec 5, 2017, at 10:16 AM, microscopy.listserver-at-gmail.com wrote:
}
} Email: driscollg2-at-winthrop.edu Name: Garrett Driscoll
}
} Organization: Winthrop University
}
} Title-Subject: [Filtered] Embedding Chick Brain (E7-E10) for Vibratome Sectioning
}
} Message: I am doing brain electroporations with an RCASB plasmid and I wanted to visualize how
} efficient my electroporations were. Currently, I am embedding my heads in 3% agarose at 37 degrees
} between 15-20 minutes, cutting the block fairly close to the brain, and a final embedding in 6%
} agarose at 4 degrees for at least 20 minutes. The smallest sections I have been able to slice is 300
} microns; however, I would like to get down to 200 microns. When I have attempted to go below 300
} microns, the tissue gets destroyed, which I can clearly see that it's lacking structural support
} from how I am embedding.
} My question: What would be a more efficient way to embed my samples so I can section between 100-200
} microns?
}


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From: microscopy.listserver-at-gmail.com
Date: Sun, 10 Dec 2017 10:19:04 -0600
Subject: [Microscopy] viaWWW:Embedding Chick Brain (E7-E10) for Vibratome Sectioning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: arvind-at-nbrc.ac.in

Hi,
you can try Celloidin embedding media, with that you can section as thin
as 15 micron I had used it for embedding Golgi stained human and mouse brain tissue.

here is the link for detailed description hope it helps you

http://stainsfile.info/StainsFile/prepare/process/celloidin.htm



best regards,

Arvind Singh Pundir
National Brain Research Centre,
Manesar, Gurgaon
Haryana- INDIA

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Email: driscollg2-at-winthrop.edu Name: Garrett Driscoll

Organization: Winthrop University

Title-Subject: [Filtered] Embedding Chick Brain (E7-E10) for Vibratome
Sectioning

Message: I am doing brain electroporations with an RCASB plasmid and I
wanted to visualize how
efficient my electroporations were. Currently, I am embedding my heads in
3% agarose at 37 degrees
between 15-20 minutes, cutting the block fairly close to the brain, and a
final embedding in 6%
agarose at 4 degrees for at least 20 minutes. The smallest sections I have
been able to slice is 300
microns; however, I would like to get down to 200 microns. When I have
attempted to go below 300
microns, the tissue gets destroyed, which I can clearly see that it's
lacking structural support
from how I am embedding.
My question: What would be a more efficient way to embed my samples so I
can section between 100-200
microns?



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From: microscopy.listserver-at-gmail.com
Date: Sun, 10 Dec 2017 10:19:48 -0600
Subject: [Microscopy] Inert Sample Transfer for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Reuschle, David (D) {DReuschle-at-dow.com}

Email: dreuschle-at-dow.com {mailto:dreuschle-at-dow.com} Name: David Reuschle

Organization: Dow Chemical

Title-Subject: Inert Sample Transfer for SEM

Message: I would like to get some advice/input from folks that have worked with air sensitive and
moisture sensitive samples for SEM-EDS. What are some of the methods and best practices to get the
sample from the glovebox into the SEM without exposing, and thus modifying, the sample? Thanks in
advance!


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From: microscopy.listserver-at-gmail.com
Date: Sun, 10 Dec 2017 10:20:32 -0600
Subject: [Microscopy] viaWWW:

Contents Retrieved from Microscopy Listserver Archives
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X-from: Calabrese, Michael {Michael.Calabrese-at-Teledyne.com}
Is there adequate water flow in the DP site glass? Are the top of the cooling coils on the DP cool
to the touch? Occasionally the reducer in the water line gets clogged with debris.


Mike

-----Original Message-----
X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Tuesday,
December 05, 2017 10:48 AM
To: Calabrese, Michael

X-from: pveril-at-uth.gr

This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at
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Email: pveril-at-uth.gr Name: Panagiotis Berillis

Organization: University of Thessaly

Title-Subject: [Filtered] CM10 ODP water message

Message: Hi all
Today I tried to switch on our Philips CM10 TEM. After an hour the message "ODP water" appeared on
the monitor. Reading the manual I figured out that the message was about high water temperature (} 60
oC)on the ODP cooling system. There is the S30 switch that checks that temperature. Our water
cooling system seems to work fine (temperature about 15oC). Any ideas how to fix the problem?

Panagiotis

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From: microscopy.listserver-at-gmail.com
Date: Sun, 10 Dec 2017 10:21:10 -0600
Subject: [Microscopy] Re: EDS issue high intensity low energy peak

Contents Retrieved from Microscopy Listserver Archives
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X-from: Erico Freitas {freitas.erico-at-gmail.com}


Dear all


We got our EDAX system fixed. At the end we needed to replace the data processing board. Fortunately
we had one available in out lab from another EDAX system.

Thanks for the suggestions anyway


Regards,

Erico Freitas
Físico/Physicist
Centro de Microscopia
Universidade Federal de Minas Gerais

Coordenador do Lab. de Microscopia Eletrônica dr Transmissão

On Nov 21, 2017 12:45 AM, {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} }
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X-from:         Ferenc Molnar {ferenc.l.molnar-at-googlemail.com
{mailto:ferenc.l.molnar-at-googlemail.com} }


Dear Erico,

this sounds to me as there is an issue with additional light in the chamber. Make sure that all
light sources - especially chamber scope - are turned off. A little bit tricky might be a touch
alarm: on some SEM (do we talk about SEM?) the stage alarm triggers some safety light barrier in
order to prevent another collision. To turn these lights off, you have to initialize the stage.

If you can exclude additional light in the chamber, there is maybe an issue with the detector: it
can be the cooling system or some issue with the preamp of the SDD/SiLi.

I hope that helps. In general, EDS detectors are very sensible to all kind of light, not only the
Xray you want to see.


With best regards,
Ferenc

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2017 um 16:38 Uhr:




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{mailto:freitas.erico-at-gmail.com {mailto:freitas.erico-at-gmail.com} } }


     Dear,


     Our EDS is showing a high intensify peak at about 0.2 keV even with beam on vacuum. We're not
     getting C signal anymore and the energy resolution is getting worse. There'is been an energy
     shift also.
     The issue remains after run an automatic callibraion.
     Does anybody know what more could be done? Or is it a sign that our detector is almost dead?

     Regards,

     Erico Freitas
     Físico/Physicist
     Centro de Microscopia
     Universidade Federal de Minas Gerais

     Coordenador do Lab. de Microscopia Eletrônica dr Transmissão

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--
Ferenc Molnar
Physiker / physicist
Schwetzingen (Germany)

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From: microscopy.listserver-at-gmail.com
Date: Sun, 10 Dec 2017 10:23:27 -0600
Subject: [Microscopy] Fwd: viaWWW:coating for Carbon evaporator bell

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Bentley, Karen {Karen_Bentley-at-URMC.Rochester.edu}

Have you fixed the tissue in paraformaldehyde?
Karen Bentley

Karen Bentley, M.S.
Director
Electron Microscope Shared Resource Laboratory
Pathology & Laboratory Medicine
University of Rochester Medical Center
575 Elmwood Avenue
Rochester, NY 14642
585-275-1954



-----Original Message-----
X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Tuesday,
December 05, 2017 1:23 PM
To: Bentley, Karen {Karen_Bentley-at-URMC.Rochester.edu}

X-from: John Nailon {jvnailon-at-gmail.com}


Hi Sylvian,

Every time you use the evaporator to coat a sample with carbon or metal everything gets coated i
eluding the inside of the glass bell jar, all of the electrodes and all of the insulated
connections. If you do not clean the system periodically then you will start to have slow pump down
times, inability to see inside the bell jar, and potential short circuits across the insulated
connections within the bell jar.

Regards
John V Nailon
Retired Electron Microscopist

John V Nailon
Mob: 0423 020 680
Email: jvnailon-at-gmail.com {mailto:jvnailon-at-gmail.com}


On Tue, Dec 5, 2017 at 3:22 AM, {microscopy.listserver-at-gmail.com
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X-from: Sylvain Poudrette {spoudrette-at-videotron.ca {mailto:spoudrette-at-videotron.ca} }


Hi

I have a coater i'm refurbishing and wondering what this detergent coating is for ?

Sylvain Poudrette

Le 2017-12-03 角 11:45 AM, microscopy.listserver-at-gmail.com
{mailto:microscopy.listserver-at-gmail.com} a 谷crit :

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} X-from:    John Nailon {jvnailon-at-gmail.com {mailto:jvnailon-at-gmail.com} }
}
}
} G'day Matthew,
}
} It is very simple and inexpensive, clean your bell jar, electrodes, and insulators as best
you can
} then wipe the inside with a piece of paper towel that has had 5-10ml of dishwashing detergent
} applied to it. Wipe the whole of the glass, metal and insulator surfaces so that there is a very
} thin smear of detergent applied. Pump down the bell jar to dry the system out. The system is
ready
} to use!
} Next time you need to clean the system all you need do is wash all surfaces with warm water, dry
} them off with paper towel, then apply the detergent again.
}
} Regards
} John V Nailon
} Retired Electron Microscopist
}
} John V Nailon
} Mob: 0423 020 680
} Email: jvnailon-at-gmail.com {mailto:jvnailon-at-gmail.com} {mailto:jvnailon-at-gmail.com
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}
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}      Organization: Center for Advanced Microscopy & Imaging / Miami University
}
}      Title-Subject: [Filtered] coating for Carbon evaporator bell jar
}
}      Message: Afternoon all,
}
}      In a previous post I asked what everyone was doing for coating the bell jar on your carbon
}      evaporator, now that Bell Bright wasn*t going to be available.  It seemed the general
consensus was
}      to use dish soap as a replacement. Well, I*ve exhausted my hoard and like a dummy I
didn*t ask for
}      how you did that.  So the obvious questions are what are you using instead of Bell
Bright and
} how do
}      you apply it?
}
}      TIA
}
}      Matthew L. Duley
}
}      Microscopy Specialist
}      Center for Advanced Microscopy & Imaging
}      9B Upham Hall
}      Miami University
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From: microscopy.listserver-at-gmail.com
Date: Sun, 10 Dec 2017 10:29:11 -0600
Subject: [Microscopy] viaWWW: CM10 ODP water message

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Ribardire Michel {m.ribardiere-at-jeol.fr}

Panagiotis,

It could .be the sensor itself problem, but mostly Can come from the water Flow , or pressure too
low To get the necessary Flow rate
Regards
Michel





Envoy depuis mon appareil mobile Samsung.


-------- Message d'origine --------
De : microscopy.listserver-at-gmail.com
Date : 05/12/2017 19:11 (GMT+01:00)
: Ribardire Michel {m.ribardiere-at-jeol.fr}
Objet : [Microscopy] viaWWW:




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Email: pveril-at-uth.gr Name: Panagiotis Berillis

Organization: University of Thessaly

Title-Subject: [Filtered] CM10 ODP water message

Message: Hi all
Today I tried to switch on our Philips CM10 TEM. After an hour the message "ODP water" appeared on
the monitor. Reading the manual I figured out that the message was about high water temperature (} 60
oC)on the ODP cooling system. There is the S30 switch that checks that temperature. Our water
cooling system seems to work fine (temperature about 15oC). Any ideas how to fix the problem?

Panagiotis

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From: bigelow-at-umich.edu
Date: Sun, 10 Dec 2017 22:07:30 -0600
Subject: [Microscopy] Re: Specimen transfer rod

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have designed specimen rods that protect specimens from air during
transfer between a glove box and the microscope. If I can be of help let
me know.

Wil Bigelow

--
Wilbur C. Bigelow, PhD, LCDR USNR (Ret)
Professor Emeritus of Materials Engineering
The University of Michigan
2911 Whittier Court, Ann Arbor MI 48104


==============================Original Headers==============================
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From: microscopy.listserver-at-gmail.com
Date: Mon, 11 Dec 2017 06:37:16 -0600
Subject: [Microscopy] Specimen transfer rod

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: bigelow-at-umich.edu
----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

I have designed specimen rods that protect specimens from air during transfer between a glove box
and the microscope. If I can be of help let me know.

Wil Bigelow

--
Wilbur C. Bigelow, PhD, LCDR USNR (Ret)
Professor Emeritus of Materials Engineering
The University of Michigan
2911 Whittier Court, Ann Arbor MI 48104

==============================Original Headers==============================
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From: microscopy.listserver-at-gmail.com
Date: Mon, 11 Dec 2017 06:38:09 -0600
Subject: [Microscopy] Fwd: Re: Inert Sample Transfer for SEM

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-------- Forwarded Message --------
X-from: Johnson, Bradley R {Bradley.Johnson-at-pnnl.gov}

Hello All,
We do some work with Li-ion batteries. I built an airlock chamber for our SEM that I would purge
with N2 or Ar; and then we used the South Bay Technologies Sample Savers to transfer the specimen
from the glove box to the airlock chamber on the SEM. It was adequate.

-Brad




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From: microscopy.listserver-at-gmail.com
Date: Mon, 11 Dec 2017 06:38:37 -0600
Subject: [Microscopy] Fwd: Re: Inert Sample Transfer for SEM

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-------- Forwarded Message --------
X-from: Rich Hailstone {hailstone-at-cis.rit.edu}


Quantomix capsules from the EM suppliers might work. You can seal them in the glovebox and easily
transport them to the SEM.

Rich


On 12/10/2017 11:41 AM, microscopy.listserver-at-gmail.com wrote:
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} X-from: Reuschle, David (D) {DReuschle-at-dow.com}
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} Email: dreuschle-at-dow.com {mailto:dreuschle-at-dow.com}   Name: David Reuschle
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} Organization: Dow Chemical
}
} Title-Subject: Inert Sample Transfer for SEM
}
} Message: I would like to get some advice/input from folks that have worked with air sensitive and
} moisture sensitive samples for SEM-EDS.  What are some of the methods and best practices to get the
} sample from the glovebox into the SEM without exposing, and thus modifying, the sample?  Thanks in
} advance!
}
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--
Richard Hailstone
Associate Professor
Center for Imaging Science
College of Science
Rochester Institute of Technology
Carlson Hall, Rm 2239
54 Lomb Memorial Dr
Rochester, NY
Office: 585-475-6306
Email: hailstone-at-cis.rit.edu
Web: https://www.rit.edu/cis/nanoimaging/


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From: klivi-at-jhu.edu
Date: Wed, 13 Dec 2017 22:30:42 -0600
Subject: [Microscopy] Job Opportunity at Johns Hopkins University, Baltimore

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I would be interested in hearing or seeing more about some of these designs.

We had a chamber built for our JEOL 840A that mated with its load-lock. There was always some question about the atmosphere in the load-lock that we were exposing the sample to during pump-down. It certainly limited exposure to air.

There are at least two commercial products that might be suitable. I have no vested interest in them.
https://www.quorumtech.com/quorum-product/pp3004-quicklok-ambient-temperature-airlock
https://www.kammrath-weiss.com/en/products/materials/transfer-module.html

Currently we are running a Quant 250. We do not have a means to positively control the opening or a door or hatch. We made a small chamber with a swing-away door on the top. We load the chamber and close the door in a glove box and pump down the chamber. We mount the chamber in the SEM and a torsion spring swings the door open when chamber pressure gets low enough in the SEM. It does pretty well and would do even better if we could guarantee that the SEM was pumping down from an inert atmosphere.

Warren Straszheim
Materials Analysis and Research Lab
Iowa State University

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I have designed specimen rods that protect specimens from air during transfer between a glove box and the microscope. If I can be of help let me know.

Wil Bigelow

--
Wilbur C. Bigelow, PhD, LCDR USNR (Ret)
Professor Emeritus of Materials Engineering The University of Michigan
2911 Whittier Court, Ann Arbor MI 48104

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The Department of Materials Science and Engineering (DMSE) and the Materials Characterization and Processing (MCP) Facility of Johns Hopkins University have an opening for an Associate Staff Engineer who will be responsible for operation, training (including safety), support, purchasing and maintenance of equipment within the Department and the MCP. The successful candidate will have proficiency in Scanning Electron Microscopy (required), X-ray Diffractometry (required), X-ray Photoelectron and Auger Spectrometry (recommended). There is a significant educational component to this position an ample opportunity to engage with students’ and faculty’s research. We are looking for a creative problem solver who enjoys working with people and delving into a wide spectrum of interesting research.

Qualificiations:

Requires a Bachelor’s degree in engineering. Master’s degree preferred. Experience operating and maintaining a significant subset of the apparatus within the MCP, especially scanning electron microscopes and scientific analysis software. Experience in training in the use of scientific equipment.

For a more complete description of the job and its requirements, please visit:

https://career4.successfactors.com/sfcareer/jobreqcareer?jobId=5689&company=jhu&username

The position is open immediately and will remain online until filled.

Johns Hopkins University is an Equal Opportunity Employer

Kenneth JT Livi, PhD
Director, Materials Characterization and Processing Center
Materials Science and Engineering
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From: microscopy.listserver-at-gmail.com
Date: Fri, 15 Dec 2017 20:45:27 -0600
Subject: [Microscopy] viaWWW:beam current instability on JEOL 2010

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Email: tomw-at-uidaho.edu Name: Tom Williams

Organization: Univ of Idaho

Title-Subject: [Filtered] beam current instability on JEOL 2010

Message: Greetings,

My JEOL 2010 TEM [running a LaB6] is experiencing beam current instability in "dark current" mode
[filament heater -at- 0/no beam]. Under nominal conditions the dark current should run at 1/2 the kV
setting [e.g. 100 microamps -at- 200 kV]. We lost the beam unexpected due to a sample issue and when I
was running the voltage back up to 200 kV the dark current began to increase above the nominal value
at 180 kV. Dark current was stable at 90 microamps [180 kV]. At 188 kV current jumped to 108
microamps. Running the kV back to 180 reverses the problem and the current stabilizes over the
course of 1-2 hours.

Note: LaB6 is at the end of its service life and is scheduled for replacement.

This is an issue we experienced with this scope long ago when I had a service contract and the
engineer would handle it during a service visit. We no longer have a contract [familiar theme] and
I cannot recall ways to address this issue other than "resting" the HT system. The SF6 gas
pressures seem normal in the HT tank and the gun.

Any suggestions, procedures or comments would be appreciated.

Thanks,
Tom


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From: microscopy.listserver-at-gmail.com
Date: Fri, 15 Dec 2017 20:46:31 -0600
Subject: [Microscopy] viaWWW:Two large TEMs looking for a new home

Contents Retrieved from Microscopy Listserver Archives
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Email: alexandergreene-at-sbcglobal.net Name: Alex Greene

Organization: Scientific Instrumentation Services, Inc.

Title-Subject: [Filtered] Two large TEMs looking for a new home

Message: As a few of you may know, my company deals in older electron microscopes. We service them
and restore them and have been in the instrument field for over 45 years. We have two heavy TEMs
that are looking for a new home and you may have either one for the cost of freight. The really
large one is an impressive JEOL 4000-EX, 400kV Transmission Electron Microscope and the other one is
a Philips EM-430, 300 kV Transmission Electron Microscope. We had a 430 at the University of
Illinois and I actually imaged 2.04, 1.4 and .07 Angstrom Gold just after it was taken out of the
crate and set up. Philips typically underrated their wonderful instruments. Anyway, these two
microscopes are free, with the exception of any shipping costs. Also, the deal is predicated on us
actually getting possession of the microscopes and that is a 90% sure deal.

Thank you and Merry Christmas, Happy Holidays and Happy New Year.

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From: microscopy.listserver-at-gmail.com
Date: Fri, 15 Dec 2017 20:47:16 -0600
Subject: [Microscopy] viaWWW:Metal evaporation deposition units

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Email: gary-at-microtechnics.com Name: Gary Gaugler

Organization: Microtechnics

Title-Subject: [Filtered] Metal evaporation deposition units

Message: Are there some suggestions about high vacuum evaporation deposition systems? I'm looking
for something that would do high resolution deposition of Pt and Ir but does not have a large foot
print like the Denton 502 units.

The Denton Desk IV TSC using Ir does not produce a fine enough coating for imaging at 25KX+. I've
tried 15mT, 35% current and 60 seconds time but get clumps in my images using FEGSEM.

Any ideas or suggestions please?


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From: microscopy.listserver-at-gmail.com
Date: Fri, 15 Dec 2017 20:47:58 -0600
Subject: [Microscopy] viaWWW:Reducing autofluorescence in embryonic chick brain

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Email: driscollg2-at-winthrop.edu Name: Garrett Driscoll

Organization: Winthrop University

Title-Subject: [Filtered] Reducing autofluorescence in embryonic chick brain
Message: I am trying to reduce the amount of autofluorescence from my embryonic chick brains (E10
and E12), which are being imaged by light-sheet microscopy. Currently, I am drop fixing my heads in
4% PFA. The embryos hearts are too delicate for us to perfuse them, so I was hoping to have another
method to remove hemoglobin from the brain and that should reduce my autofluorescence. I have both
GFP and CTB alexa fluor 555 that I am looking at with my imaging. Additionally, I am doing a BABB
clearing method.

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From: microscopy.listserver-at-gmail.com
Date: Sun, 17 Dec 2017 13:53:25 -0600
Subject: [Microscopy] viaWWW:Ziess Leo Supra 25 - L REM fault

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Email: mplattsc-at-gmail.com Name: Michael Platt

Organization: University of South Carolina

Title-Subject: [Filtered] Ziess Leo Supra 25 - L REM fault

Message: The L REM fault is probably caused by power supply failures. Not being familiar with this
SEM and without schematics and layout drawings, I need some guidance as to where these supplies are
located and typically what goes wrong with them.


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From: microscopy.listserver-at-gmail.com
Date: Sun, 17 Dec 2017 13:57:06 -0600
Subject: [Microscopy] Fwd: viaWWW:Reducing autofluorescence in embryonic chick brain

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X-from: Glen MacDonald {glenmac-at-u.washington.edu}

Try 1% Sodium Borohydride in PBS for 10-15 min.
Clancy and Cauller, 1998
Won’t kill the fluorescent proteins like some of the other treatments.
Regards,
Glen MacDonald
Digital Microscopy Center
Box 357923
University of Washington
Seattle, WA 98195-7923 USA
(206) 616-4156
depts.washington.edu/digmicro
glenmac-at-uw.edu









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} Title-Subject: [Filtered] Reducing autofluorescence in embryonic chick brain
} Message: I am trying to reduce the amount of autofluorescence from my embryonic chick brains (E10
} and E12), which are being imaged by light-sheet microscopy. Currently, I am drop fixing my heads in
} 4% PFA. The embryos hearts are too delicate for us to perfuse them, so I was hoping to have another
} method to remove hemoglobin from the brain and that should reduce my autofluorescence. I have both
} GFP and CTB alexa fluor 555 that I am looking at with my imaging. Additionally, I am doing a BABB
} clearing method.
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From: microscopy.listserver-at-gmail.com
Date: Mon, 18 Dec 2017 06:41:34 -0600
Subject: Re: Sodiumborohydride and aldehydefixed cells

Contents Retrieved from Microscopy Listserver Archives
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X-from: Cammer, Michael {Michael.Cammer-at-med.nyu.edu}

found here a few years ago:


X-from: "James F. Sanzo" sanzo-at-AVIGENICS.COM {mailto:sanzo-at-AVIGENICS.COM}

There are several other methods you can try that quench the remaining free aldehyde groups left
after glutaraldehyde fixation (below). However, if you are not necessarily interested in
ultrastructure, why not use paraformaldehyde? Aldehyde induced fluorescence is usually much less of
a problem with the PFA. You may also want to think about whether the fluorescence you are seeing is
indeed due to aldehydes - or if it could be due to some other cellular constituent like lysolipids.
As an alternative, you may also want to consider using the longest wavelength your imaging and
fluorophore systems will allow. Try looking at the background fluorescence above around 590nm. It
may be all you need to do.

For blocking free aldehydes remaining after fixation:

1. 1% (or 150mM) glycine with 0.1% Tween in pbs. Wash well. (glycine binds to free aldehydes)

2. 50 mM NH4Cl in PBS. Incubate for 15 min at RT. Wash well. (see Bendyan)

3. 0.1% Na borohydride in PBS. Apply while fizzing and incubate 3 x 10 min on ice. Wash well.
(reduces carbonyls)

4. 0.15 M ethanolamine, pH 7.5. Incubate 30 min on ice. Wash well.

*Also...*

X-from: Scott Snyder SSnyder-at-NIAID.NIH.GOV {mailto:SSnyder-at-NIAID.NIH.GOV}

X-from: Glen MacDonald {glenmac-at-u.washington.edu}

Try 1% Sodium Borohydride in PBS for 10-15 min.
Clancy and Cauller, 1998
Won’t kill the fluorescent proteins like some of the other treatments.
Regards,
Glen MacDonald
Digital Microscopy Center
Box 357923
University of Washington
Seattle, WA 98195-7923 USA
(206) 616-4156
depts.washington.edu/digmicro
glenmac-at-uw.edu









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} Email: driscollg2-at-winthrop.edu Name: Garrett Driscoll
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} Organization: Winthrop University
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} Title-Subject: [Filtered] Reducing autofluorescence in embryonic chick brain
} Message: I am trying to reduce the amount of autofluorescence from my embryonic chick brains (E10
} and E12), which are being imaged by light-sheet microscopy. Currently, I am drop fixing my heads in
} 4% PFA. The embryos hearts are too delicate for us to perfuse them, so I was hoping to have another
} method to remove hemoglobin from the brain and that should reduce my autofluorescence. I have both
} GFP and CTB alexa fluor 555 that I am looking at with my imaging. Additionally, I am doing a BABB
} clearing method.
}
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From: microscopy.listserver-at-gmail.com
Date: Wed, 20 Dec 2017 06:44:02 -0600
Subject: [Microscopy] viaWWW:Join our Team! Ted Pella, Inc.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello:



We have a Zeiss Libra TEM 120. We use it for biological samples. Thin sections and negative stain. We are currently having problems getting service on our current camera. It may be time for a new camera. Anyone out there have recommendations for a side mounted TEM camera with good reliable service?



Best wishes,

Carol


--
Carol Cooke
Electron Microscopy Lab Manager
Johns Hopkins University
Neurology-Peripheral Nerve Division
Neurology Microscopy Core Facility
Pathology-510
600 N. Wolfe Street
Baltimore, MD 21287
Lab Phone 410-955-1424

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From: microscopy.listserver-at-gmail.com
Date: Wed, 20 Dec 2017 06:44:47 -0600
Subject: [Microscopy] viaWWW:Open Cryo TEM Position

Contents Retrieved from Microscopy Listserver Archives
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X-from: michelle.plue-at-duke.edu

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Email: michelle.plue-at-duke.edu Name: Michelle Plue

Organization: Duke University

Title-Subject: [Filtered] Open Cryo TEM Position

Message: Hello All.

There is an opening for a Cryo TEM microscopist in the Shared Materials Instrumentation Facility
(SMIF- https://smif.pratt.duke.edu/ )at Duke University. This person will manage the daily
operation and maintenance of the Cryo-TEM and associated sample preparation equipment. They will
ensure exceptional service to the research community in a timely and efficient manner and provide
training, guidance and consultation to users of the Cryo-TEM and associated sample preparation
equipment.

The last link I sent did not work very well. The position # is 401357060 and the website is
https://hr.duke.edu/careers/apply

Cheers,
Michelle

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From: microscopy.listserver-at-gmail.com
Date: Wed, 20 Dec 2017 06:45:24 -0600
Subject: [Microscopy] viaWWW:Scanning Electron Microscopist opening

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: rcsencsits-at-belcan.com

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Email: rcsencsits-at-belcan.com Name: Roseann Csencsits

Organization: Belcan Government Services

Title-Subject: [Filtered] Scanning Electron Microscopist opening

Message: Belcan Government Services has an immediate opportunity as a Scanning Electron Microscopist
located in Sunol, CA.

Geologist or physicist preferred.

For position description and to apply:

https://belcanjobs.smartsearchonline.com/jobs_govservices/jobdetails.asp?current_page=1&location=CA&apply=yes&job_number=1219757


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From: microscopy.listserver-at-gmail.com
Date: Wed, 20 Dec 2017 06:47:03 -0600
Subject: [Microscopy] Fwd: Re: viaWWW:Metal evaporation deposition units

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Bill & Sue Tivol {wtivol-at-sbcglobal.net}

Hi Gary,
I used the Cressington 208, with the metal evaporation power supply, to evaporate Ti, which has a
very high vaporization temperature, and it performed quite well. As usual, I’m not affiliated
except as a customer.
Yours,
Bill


} On Dec 15, 2017, at 7:11 PM, microscopy.listserver-at-gmail.com wrote:
}
} X-from: gary-at-microtechnics.com
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at http://www.microscopy.com/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when replying please copy both
} gary-at-microtechnics.com as well as the Microscopy Listserver
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}
} Email: gary-at-microtechnics.com Name: Gary Gaugler
}
} Organization: Microtechnics
}
} Title-Subject: [Filtered] Metal evaporation deposition units
}
} Message: Are there some suggestions about high vacuum evaporation deposition systems? I'm looking
} for something that would do high resolution deposition of Pt and Ir but does not have a large foot
} print like the Denton 502 units.
}
} The Denton Desk IV TSC using Ir does not produce a fine enough coating for imaging at 25KX+. I've
} tried 15mT, 35% current and 60 seconds time but get clumps in my images using FEGSEM.
}
} Any ideas or suggestions please?
}
}




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From: sergei2-at-ornl.gov
Date: Thu, 21 Dec 2017 08:42:45 -0600
Subject: [Microscopy] 2 Postdoctoral positions: atom by atom fabrication by STEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues

Two postdoctoral position in the field of direct atom-by-atom
fabrication by sub-atomically focused beam of Scanning Transmission
Electron Microscope and artificial intelligence applications in STEM and
scanning probe microscopy are now posted. Please look for the position
descriptions and application forms on ORNL Job search website using :

*Position Title:* Postdoctoral Research Associate in Microscopy Control
for Atomic Scale Manipulation / NB50648778
*Position Title:* Postdoctoral Research Associate, Aberration-corrected
STEM Under Artificial Intelligence Control / NB50648194

The recent examples of the work the candidates will be involved in are
available in recent publications (arXiv:1711.05810
{https://arxiv.org/abs/1711.05810} , arXiv:1710.10338
{https://arxiv.org/abs/1710.10338} , arXiv:1710.09416
{https://arxiv.org/abs/1710.09416} , arXiv:1708.01523
{https://arxiv.org/abs/1708.01523} , arXiv:1709.00470
{https://arxiv.org/abs/1709.00470} ). The ideal candidate will have
expertise in atomically resolved STEM, image simulation, and Python
programming (for NB50648194) and STEM/SPM and machine learning (for
NB50648778).

Please contact Sergei Kalinin and Stephen Jesse directly
(sergei2-at-ornl.gov and sjz-at-ornl.gov) for additional details.

Sergei

--
Sergei V. Kalinin

Director,
Institute for Functional Imaging of Materials

Fellow MRS, IEEE, APS, AVS

Oak Ridge National Laboratory

Phone: (865) 241-0236


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From: sergei2-at-ornl.gov
Date: Thu, 21 Dec 2017 08:51:58 -0600
Subject: [Microscopy] PyCroscopy - imaging file formats, image analytics, and hyperspectral

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear colleagues - we would like to bring to your attention the GitHub
repository for the community-wide development of codes for image
analytics (cleaning, feature extraction, analysis of atomic positions),
hyperspectral unmixing of high-dimensional data (STEM-EELS,
ptychography, SPM spectroscopies), and supporting universal file format.

The codes are realized via Jupyter notebooks that are straightforward to
use. The site is:

https://pycroscopy.github.io/pycroscopy/about.html

And additional description is provided below. Join the open-code movement!

Sergei

*About*:

· Pycroscopy is a free and community-driven python
{http://www.python.org/} package for scientific analysis of imaging
modalities such as scanning transmission electron microscopy, scanning
probe microscopy, scanning tunneling spectroscopy, and x-ray diffraction
microscopy.

· Pycroscopy uses a /data-centric model/ wherein the raw data collected
from the microscope, results from analysis and processing routines are
all written to standardized /hierarchical data format (HDF5)/ files for
traceability, reproducibility, and provenance.

· Pycroscopy uses an instrument-independent /universal data format/ that
facilitates the generalized representation of data from any instrument

· The instrument-independent data format facilitates the development of a
/single and generalized version of analysis code/ to be reused for data
from a variety of sources.

· The universal data format and generalized codebase enables Pycroscopy
to serve as the hub for community-driven imaging and microscopy research.

*Motivation*:

1.Growing data sizes

* Cannot use desktop computers for analysis
* /Need: High performance computing, storage resources and
compatible, scalable file structures/

2.Increasing data complexity

* Sophisticated imaging and spectroscopy modes resulting in 5,6,7…
dimensional data
* /Need: Robust software and generalized data formatting/

3.Multiple file formats

* Different formats from each instrument. Proprietary in most cases
* Incompatible for correlation
* /Need: Open, instrument independent data format/

4.Disjoint communities

* Similar analysis routines written by each community (SPM, STEM,
TOF SIMs, XRD…) /independently/!
* /Need: Centralized repository, instrument agnostic analysis
routines that bring communities together/

5.Expensive analysis software

* Software supplied with instruments often insufficient /
incapable of custom analysis routines
* Commercial software (Eg: Matlab, Origin..) are often
prohibitively expensive.
* /Need: Free, powerful, open source, user-friendly software/

*Available tools in pycroscopy:*

·Analysis:

o Finding and refining atomic positions in atomically resolved images
o Reconstruction of current in ultra-fast current-voltage spectroscopy
via Bayesian inference
o Fitting peaks to models in hyperspectral data
o Fitting piezoresponse hysteresis loops to models

·Data processing:

o Statistical denoising of images
o Utilities to simplify clustering and decomposition of data
o Feature extraction from images
o Image transformations
o FFT signal filtering

·File and data handling:

oUtilities for advanced and file-safe writing
oAbility to read, write, manipulate multi-dimensional / hyperspectral
datasets (1D – 8D and beyond)
oTranslating from proprietary data formats:

§Band excitation imaging and spectroscopy
§Time resolved Kelvin Probe force microscopy
§I-V + First Order Reversal Curve spectroscopy
§General mode imaging and spectroscopy
§Asylum Research imaging and spectroscopy
§Nanonis imaging and spectroscopy
§Nion Co. scanning transmission electron microscopy imaging
§Omicron scanning tunneling spectroscopy
§FEI Titan ptychography / scanning transmission electron microscopy imaging
§Standard images – PNG, TIFF, etc.

·Visualization:

oTools for interactive visualization of complex multi-dimensional datasets
oUtilities for generating publication-ready plots

*Journal papers published using pycroscopy:*

1. Big Data Analytics for Scanning Transmission Electron Microscopy
Ptychography {https://www.nature.com/articles/srep26348} by S. Jesse
et al., /Scientific Reports/ (2015);
2. Rapid mapping of polarization switching through complete information
acquisition {http://www.nature.com/articles/ncomms13290} by S.
Somnath et al., /Nature Communications/ (2016);
3. Improving superconductivity in BaFe2As2-based crystals by cobalt
clustering and electronic uniformity
{http://www.nature.com/articles/s41598-017-00984-1} by L. Li et al.,
/Scientific Reports/ (2017);
4. Direct Imaging of the Relaxation of Individual Ferroelectric
Interfaces in a Tensile-Strained Film
{http://onlinelibrary.wiley.com/doi/10.1002/aelm.201600508/full} by
L. Li et al.; /Advanced Electronic Materials/ (2017),
5. Ultrafast Current Imaging via Bayesian Inference by S. Somnath et
al., accepted at /Nature Communications/ (2017).
6. Decoding apparent ferroelectricity in perovskite nanofibers by R.
Ganeshkumar et al., accepted at ACS Applied Materials & Interfaces
(2017).
7. Feature extraction via similarity search: application to atom
finding and denosing in electon and scanning probe microscopy
imaging by S. Somnath et al.; under review at /Advanced Structural
and Chemical Imaging/ (2017).

--
Sergei V. Kalinin

Director,
Institute for Functional Imaging of Materials

Fellow IEEE, MRS, APS, AVS

Oak Ridge National Laboratory

Phone: (865) 241-0236






--
Sergei V. Kalinin

Director,
Institute for Functional Imaging of Materials

Fellow MRS, APS, AVS

Oak Ridge National Laboratory

Phone: (865) 241-0236




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From: microscopy.listserver-at-gmail.com
Date: Tue, 26 Dec 2017 20:34:03 -0600
Subject: [Microscopy] viaWWW: Availability of Used TEM in Europe ?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We recently purchased a Leica ACE 600 system equipped with sputter coater,
carbon wire coater, and glow discharge options. We did not get a Pt target,
but have Ir, Cr, Au, and AuPd. I think that it has a small foot print. It
is easy to use. I use Ir. I found that the rate of deposition for a 1 nm
coating was too fast, so I created a new recipe by decreasing the current by
half to 40 mA. It takes about 30 sec and I get good coatings.

If you are getting clumps in your deposition, you should investigate more
before investing in a new unit. Check your target for wear. Check that
there is good thermal connection. Check your gas supply for purity. You
can also play around a bit with the pressure and power settings. Also, use
as long a working distance as possible.

-Scott Walck



-----Original Message-----
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Sent: Friday, December 15, 2017 10:13 PM

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Email: gary-at-microtechnics.com Name: Gary Gaugler

Organization: Microtechnics

Title-Subject: [Filtered] Metal evaporation deposition units

Message: Are there some suggestions about high vacuum evaporation deposition
systems? I'm looking for something that would do high resolution deposition
of Pt and Ir but does not have a large foot print like the Denton 502 units.

The Denton Desk IV TSC using Ir does not produce a fine enough coating for
imaging at 25KX+. I've tried 15mT, 35% current and 60 seconds time but get
clumps in my images using FEGSEM.

Any ideas or suggestions please?


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Email: hallopartha-at-gmail.com
Name: Dr. Partha Pratim Das

Organization: Electron Crystallography Solutions SL

Title-Subject: [Filtered] Availability of Used TEM

Message: Dear All,
I am working as a collaborator of Univ. of Peloponese, Greece and they
(Archaeology department of Univ. of Peloponese, Greece) are looking for
a used TEM (like older CM 100 or CM 120 with SA diffraction
capability). If any one of you know someone (institution) will be ready
to donate an used TEM, please let me know.

Best Regards

Partha

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From: microscopy.listserver-at-gmail.com
Date: Fri, 29 Dec 2017 17:05:57 -0600
Subject: [Microscopy] viaWWW:Immunogold Calretinin

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Email: Peter.Steele-at-jhmi.edu Name: P. Steele

Organization: JHACH

Title-Subject: [Filtered] Immunogold Calretinin

Message: I have a researcher looking for a lab to do immunogold for calretinin on existing clinical
epon812 blocks (Karnovsky’s, cacodylate buffer, osmium fixation, blocks cured at 70C).

Please reply to me directly (Peter.Steele-at-jhmi.edu).

Thank you.



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From: MILDRED :      westelle638-at-gmail.com
Date: Sat, 30 Dec 2017 10:21:28 -0800
Subject: re: Top 10 Ranks for microscopy.com

Contents Retrieved from Microscopy Listserver Archives
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X-from: Jan Leunissen {leunissen-at-aurion.nl}

Hello Peter,

Never say it won’t work before trying, but chances that this labeling would work would be slim in my
opinion. I would suggest the researcher has a chat with people versed in this technique (there are
quite a few that contribute to the list, may I recommend Hong Yi at Emory?). It is better to start
off with specimens that might have a better chance of success. I would be happy to help as well.

And to all of you, have a great new year!

Jan Leunissen
Aurion



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} Title-Subject: [Filtered] Immunogold Calretinin
}
} Message: I have a researcher looking for a lab to do immunogold for calretinin on existing clinical
} epon812 blocks (Karnovsky’s, cacodylate buffer, osmium fixation, blocks cured at 70C).
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