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Email: don.becker-at-bruker.com Name: Don Becker
Organization: Bruker Nano
Title-Subject: [Filtered] Job Opening at Bruker Nano Analytics
Message: Bruker Nano Analytics has an immediate opening for a Senior Sales Representative for Microanalysis in the Southeastern United States. The successful candidate will have a minimum of 3 -n 5 years experience in the sale of scientific equipment, and will sell, market, and support our microanalysis tools with new and existing customers, as well as support our OEM sales channel partners. The sales territory includes CO, NM, TX, OK, AL, NC, SC, FL, GA, LA, and MS.
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We are excited to announce this year's run of Analytical and Quantitative Light Microscopy, held at the Marine Biological Laboratory, Woods Hole, MA, USA. The course will run May 4 - May 13 , 2016.
AQLM is a comprehensive and intensive course in light microscopy for researchers in biology, medicine, and material sciences. This course provides a systematic and in-depth examination of the theory of image formation and application of digital methods for exploring subtle interactions between light and the specimen. This course emphasizes the quantitative issues that are critical to the proper interpretation of images obtained with modern wide-field and confocal microscopes.
Laboratory exercises, demonstrations, and discussions include: * geometrical and physical optics of microscope image formation including Abbe's theory of the microscope and Fourier optics; * phase contrast, polarization and interference microscopy; * fluorescence microscopy, quantification of fluorescence, and fluorescent proteins; * principles and application of digital imaging and quantitative digital image deconvolution; * digital image processing and object identification and tracking; * live cell and ratiometric imaging for FRET and ion concentration imaging; * confocal microscopy and specialized methods such as TIRF and FLIM; and * new advances in light microscopy such as FCS, PALM, STORM, SIM and SPIM.
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Posters available at http://www.mbl.edu/education/files/2014/04/AQLM_POSTER_2016v1.pdf
~~~~~~~~~~~~~~~~~~~~~~~ Wendy Salmon Light Microscopy Specialist Whitehead Institute for Biomedical Research W.M. Keck Imaging Facility 9 Cambridge Center, Rm 447 Cambridge, MA 02142 c: 617-429-0158 e: wsalmon-at-wi.mit.edu w: http://staffa.wi.mit.edu/microscopy/
==============================Original Headers============================== 12, 39 -- From wsalmon-at-wi.mit.edu Wed Jan 6 15:42:09 2016 12, 39 -- Received: from ptolemy.wi.mit.edu (ptolemy.wi.mit.edu [18.4.1.120]) 12, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u06Lg8aa011850 12, 39 -- for {microscopy-at-microscopy.com} ; Wed, 6 Jan 2016 15:42:08 -0600 12, 39 -- X-IronPort-AV: E=Sophos;i="5.20,530,1444708800"; 12, 39 -- d="scan'208";a="3335172" 12, 39 -- Received: from unknown (HELO mars.wi.mit.edu) ([10.9.4.20]) 12, 39 -- by ptolemy.wi.mit.edu with ESMTP; 06 Jan 2016 16:41:59 -0500 12, 39 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 12, 39 -- by mars.wi.mit.edu (Postfix) with ESMTP id 2518E31EB48 12, 39 -- for {microscopy-at-microscopy.com} ; Wed, 6 Jan 2016 16:41:59 -0500 (EST) 12, 39 -- Received: from mars.wi.mit.edu ([127.0.0.1]) 12, 39 -- by localhost (mars.wi.mit.edu [127.0.0.1]) (amavisd-new, port 10032) 12, 39 -- with ESMTP id 88pSmo5FAWfT for {microscopy-at-microscopy.com} ; 12, 39 -- Wed, 6 Jan 2016 16:41:58 -0500 (EST) 12, 39 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 12, 39 -- by mars.wi.mit.edu (Postfix) with ESMTP id 9E23231EB62 12, 39 -- for {microscopy-at-microscopy.com} ; Wed, 6 Jan 2016 16:41:58 -0500 (EST) 12, 39 -- X-Virus-Scanned: amavisd-new at mars.wi.mit.edu 12, 39 -- Received: from mars.wi.mit.edu ([127.0.0.1]) 12, 39 -- by localhost (mars.wi.mit.edu [127.0.0.1]) (amavisd-new, port 10026) 12, 39 -- with ESMTP id vkLbfRjMwVqd for {microscopy-at-microscopy.com} ; 12, 39 -- Wed, 6 Jan 2016 16:41:58 -0500 (EST) 12, 39 -- Received: from io.wi.mit.edu (io.wi.mit.edu [10.9.4.21]) 12, 39 -- by mars.wi.mit.edu (Postfix) with ESMTP id 83F4331EAFE 12, 39 -- for {microscopy-at-microscopy.com} ; Wed, 6 Jan 2016 16:41:58 -0500 (EST) 12, 39 -- Date: Wed, 6 Jan 2016 16:41:58 -0500 (EST) 12, 39 -- From: Wendy Salmon {wsalmon-at-wi.mit.edu} 12, 39 -- To: microscopy-at-microscopy.com 12, 39 -- Message-ID: {816105877.10462683.1452116518458.JavaMail.zimbra-at-wi.mit.edu} 12, 39 -- In-Reply-To: {1538907122.10462611.1452116467905.JavaMail.zimbra-at-wi.mit.edu} 12, 39 -- Subject: LM - 2016 Analytical and Quantitative Light Microscopy Course -at- MBL 12, 39 -- MIME-Version: 1.0 12, 39 -- Content-Type: text/plain; charset=utf-8 12, 39 -- Content-Transfer-Encoding: 7bit 12, 39 -- X-Originating-IP: [10.9.4.20] 12, 39 -- X-Mailer: Zimbra 8.0.7_GA_6021 (ZimbraWebClient - GC47 (Mac)/8.0.7_GA_6021) 12, 39 -- Thread-Topic: LM - 2016 Analytical and Quantitative Light Microscopy Course -at- MBL 12, 39 -- Thread-Index: 3I4RFXAc6NuHKgHiTFr8kP+ljEXmnA== ==============================End of - Headers==============================
Hello All, We have a trouble with our old MegaViewII TEM camera. Just before the end of the last year, the power source of a PC we are using with MegaViewII camera has gone. Just now we replaced the power source with a new one. All seemed to be OK but when we tried to record the image with MegaViewII camera we got a strange result. The image can be seen on this link:
-- Oldrich Benada Institute of Microbiology CAS, v.v.i. Videnska 1083 142 20 Prague 4 Czech Republic
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==============================Original Headers============================== 10, 42 -- From benada-at-biomed.cas.cz Thu Jan 7 08:45:03 2016 10, 42 -- Received: from barracuda.biomed.cas.cz (barracuda.biomed.cas.cz [147.231.40.11]) 10, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u07Ej2X8016084 10, 42 -- for {microscopy-at-microscopy.com} ; Thu, 7 Jan 2016 08:45:03 -0600 10, 42 -- X-ASG-Debug-ID: 1452177892-05011e5425340270001-4CH8be 10, 42 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) by barracuda.biomed.cas.cz with ESMTP id cndEuIYa6CHL3S7G (version=TLSv1 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) for {microscopy-at-microscopy.com} ; Thu, 07 Jan 2016 15:44:52 +0100 (CET) 10, 42 -- X-Barracuda-Envelope-From: benada-at-biomed.cas.cz 10, 42 -- X-Barracuda-Effective-Source-IP: mail2.biomed.cas.cz[147.231.40.32] 10, 42 -- X-Barracuda-Apparent-Source-IP: 147.231.40.32 10, 42 -- Received: from u117ob02 (c111jn.mbu.cas.cz [147.231.44.12]) 10, 42 -- (using TLSv1 with cipher AES128-SHA (128/128 bits)) 10, 42 -- (No client certificate requested) 10, 42 -- by mail2.biomed.cas.cz (Postfix) with ESMTPSA id 4D04B2C8070 10, 42 -- for {microscopy-at-microscopy.com} ; Thu, 7 Jan 2016 15:44:48 +0100 (CET) 10, 42 -- Date: Thu, 7 Jan 2016 15:44:47 +0100 10, 42 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 10, 42 -- To: {microscopy-at-microscopy.com} 10, 42 -- Subject: MegaViewII Trouble 10, 42 -- Message-ID: {20160107154447.26f75438-at-u117ob02} 10, 42 -- X-ASG-Orig-Subj: MegaViewII Trouble 10, 42 -- Organization: =?UTF-8?B?TWlrcm9iaW9sb2dpY2vDvSDDunN0YXY=?= AV 10, 42 -- =?UTF-8?B?xIxS?= 10, 42 -- X-Mailer: Claws Mail 3.11.1 (GTK+ 2.24.25; i586-pc-linux-gnu) 10, 42 -- MIME-Version: 1.0 10, 42 -- Content-Type: text/plain; charset=US-ASCII 10, 42 -- Content-Transfer-Encoding: 7bit 10, 42 -- X-IoP-CAS-MailScanner-ID: 4D04B2C8070.90340 10, 42 -- X-IoP-CAS-MailScanner: Processed 10, 42 -- X-Spam-Status: No 10, 42 -- X-Barracuda-Connect: mail2.biomed.cas.cz[147.231.40.32] 10, 42 -- X-Barracuda-Start-Time: 1452177892 10, 42 -- X-Barracuda-Encrypted: DHE-RSA-AES256-SHA 10, 42 -- X-Barracuda-URL: https://barracuda.biomed.cas.cz:443/cgi-mod/mark.cgi 10, 42 -- X-Barracuda-Scan-Msg-Size: 1664 10, 42 -- X-Virus-Scanned: by bsmtpd at biomed.cas.cz 10, 42 -- X-Barracuda-BRTS-Status: 1 10, 42 -- X-Barracuda-Spam-Score: 0.00 10, 42 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using per-user scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=9.0 tests= 10, 42 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.25917 10, 42 -- Rule breakdown below 10, 42 -- pts rule name description 10, 42 -- ---- ---------------------- -------------------------------------------------- ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mlibbee-at-lbl.gov as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: mlibbee-at-lbl.gov Name: Marissa
Organization: LBL
Title-Subject: [Filtered] Glove box
Message: To those of you who transfer samples to a TEM holder in a glove box, will you please contact me offline with the name of the glove box supplier?
Thanks!
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==============================Original Headers============================== 15, 40 -- From microscopy.listserver-at-gmail.com Thu Jan 7 14:55:16 2016 15, 40 -- Received: from mail-qg0-f48.google.com (mail-qg0-f48.google.com [209.85.192.48]) 15, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u07KtEBH010432 15, 40 -- for {microscopy-at-microscopy.com} ; Thu, 7 Jan 2016 14:55:15 -0600 15, 40 -- Received: by mail-qg0-f48.google.com with SMTP id e32so247793076qgf.3 15, 40 -- for {microscopy-at-microscopy.com} ; Thu, 07 Jan 2016 12:55:05 -0800 (PST) 15, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 15, 40 -- d=gmail.com; s=20120113; 15, 40 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 15, 40 -- :mime-version:in-reply-to:content-type:content-transfer-encoding; 15, 40 -- bh=wzdhJD5Y9ce3fflsfduzcRkZDzpQXc6DhbYciIWEdRk=; 15, 40 -- b=XigH4yoXVV5cMAHme/ZHgnkavbdBQwya05fLDWZkF17f4o27MF80LMPK4uf/9aPcci 15, 40 -- zWzKhPfTcWVpUciLjVyMH9TDRTkzkcxwZ1ROKtZ64kUz1Si9Fc6ZA4hiACcFKNvYjvvF 15, 40 -- 0swWFJi7jZ3Q5CUqurYB1DkVIamxlGos14AxNMq806c4CoLIULHxzpXavMXiACVcUm32 15, 40 -- crVMJJtGv/rFR6kADa3lwLFcjZvwSmOXuzcC567WTl+cO0RclC8hLrPGk3BEb/yYwOO6 15, 40 -- rVoTRp9sDO8cfUZf0hX8jqQotozwfhP6TPM8qulm/MKZUHPf3unGiKTCYq/c5YP/XETe 15, 40 -- H06g== 15, 40 -- X-Received: by 10.140.174.2 with SMTP id u2mr80610201qhu.62.1452200105375; 15, 40 -- Thu, 07 Jan 2016 12:55:05 -0800 (PST) 15, 40 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24]) 15, 40 -- by smtp.googlemail.com with ESMTPSA id y104sm18387621qgd.33.2016.01.07.12.55.04 15, 40 -- for {microscopy-at-microscopy.com} 15, 40 -- (version=TLSv1/SSLv3 cipher=OTHER); 15, 40 -- Thu, 07 Jan 2016 12:55:04 -0800 (PST) 15, 40 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 15, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply 15, 40 -- {microscopylistserver-noreply-at-microscopy.com} 15, 40 -- Subject: viaWWW: TEM Glove box sample transfer 15, 40 -- References: {201601071953.u07JrlLx009792-at-ns.microscopy.com} 15, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 15, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 15, 40 -- X-Forwarded-Message-Id: {201601071953.u07JrlLx009792-at-ns.microscopy.com} 15, 40 -- Message-ID: {568ED0A7.4080400-at-microscopy.com} 15, 40 -- Date: Thu, 7 Jan 2016 14:55:03 -0600 15, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 15, 40 -- Gecko/20100101 Thunderbird/38.5.0 15, 40 -- MIME-Version: 1.0 15, 40 -- In-Reply-To: {201601071953.u07JrlLx009792-at-ns.microscopy.com} 15, 40 -- Content-Type: text/plain; charset=windows-1252; format=flowed 15, 40 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Hello Warren, Ravi and Larry, Thank you for your comments and suggestions. Yesterday I forgot one important think, the pattern (image) that we are getting from megaViewII does not depend on electron beam intensity. Even when the beam is switched off, we get this pattern (image).
Best regards Oldrich
On Thu, 7 Jan 2016 15:58:40 +0000, "Straszheim, Warren E [BIOTC]" {wesaia-at-iastate.edu} wrote : } It looks like the synchronization has gone out. It is like what I see } on our SEM when the raster on the sample doesn't match the raster on } the screen. } } I presume the system is operating in TEM mode and that the MegaView } is a CCD camera. If so, the only way that such a phenomenon might } occur is if the number of pixels per line is not matching between the } CCD and display. It seems the number of pixels across your image is } slightly less than the number being read across the CCD. Therefore, } each successive line is shifted some to the right. } } Is the sample a nice simple structure? I would wonder what a single } vertical grid bar would look like. } } Warren } } -----Original Message----- } From: benada-at-biomed.cas.cz [mailto:benada-at-biomed.cas.cz] } Sent: Thursday, January 07, 2016 8:48 AM } To: Straszheim, Warren E [BIOTC] } Subject: [Microscopy] MegaViewII Trouble } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello All, } We have a trouble with our old MegaViewII TEM camera. Just before the } end of the last year, the power source of a PC we are using with } MegaViewII camera has gone. Just now we replaced the power source with } a new one. All seemed to be OK but when we tried to record the image } with MegaViewII camera we got a strange result. The image can be seen } on this link: } } http://www2.biomed.cas.cz/~benada/MegaViewII_trouble.html } } } Please does anybody had to solve such problem? } } Thanking for any response in advance. } Oldrich } } -- } Oldrich Benada } Institute of Microbiology CAS, v.v.i. } Videnska 1083 } 142 20 Prague 4 } Czech Republic
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==============================Original Headers============================== 7, 49 -- From benada-at-biomed.cas.cz Fri Jan 8 02:13:04 2016 7, 49 -- Received: from barracuda.biomed.cas.cz (barracuda.biomed.cas.cz [147.231.40.11]) 7, 49 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u088D3op013161 7, 49 -- for {microscopy-at-microscopy.com} ; Fri, 8 Jan 2016 02:13:04 -0600 7, 49 -- X-ASG-Debug-ID: 1452240773-05011e5425351cb0001-4CH8be 7, 49 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) by barracuda.biomed.cas.cz with ESMTP id oykzTSJlFOKTZZTf (version=TLSv1 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); Fri, 08 Jan 2016 09:12:53 +0100 (CET) 7, 49 -- X-Barracuda-Envelope-From: benada-at-biomed.cas.cz 7, 49 -- X-Barracuda-Effective-Source-IP: mail2.biomed.cas.cz[147.231.40.32] 7, 49 -- X-Barracuda-Apparent-Source-IP: 147.231.40.32 7, 49 -- Received: from u117ob02 (c111jn.mbu.cas.cz [147.231.44.12]) 7, 49 -- (using TLSv1 with cipher AES128-SHA (128/128 bits)) 7, 49 -- (No client certificate requested) 7, 49 -- by mail2.biomed.cas.cz (Postfix) with ESMTPSA id 92E1E2C80FA; 7, 49 -- Fri, 8 Jan 2016 09:12:49 +0100 (CET) 7, 49 -- Date: Fri, 8 Jan 2016 09:12:49 +0100 7, 49 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 7, 49 -- To: "Straszheim, Warren E [BIOTC]" {wesaia-at-iastate.edu} , 7, 49 -- {microscopy-at-microscopy.com} , {les-at-zsgenetics.com} , 7, 49 -- Ravi Thakkar 7, 49 -- {ravi.thakkar369-at-gmail.com} 7, 49 -- Subject: Re: [Microscopy] MegaViewII Trouble 7, 49 -- Message-ID: {20160108091249.40d565b0-at-u117ob02} 7, 49 -- X-ASG-Orig-Subj: Re: [Microscopy] MegaViewII Trouble 7, 49 -- In-Reply-To: {CY1PR04MB1813866E43A724B10C93B3B1D7F50-at-CY1PR04MB1813.namprd04.prod.outlook.com} 7, 49 -- References: {201601071447.u07Elg9u016882-at-ns.microscopy.com} 7, 49 -- {CY1PR04MB1813866E43A724B10C93B3B1D7F50-at-CY1PR04MB1813.namprd04.prod.outlook.com} 7, 49 -- Organization: =?UTF-8?B?TWlrcm9iaW9sb2dpY2vDvSDDunN0YXY=?= AV 7, 49 -- =?UTF-8?B?xIxS?= 7, 49 -- X-Mailer: Claws Mail 3.11.1 (GTK+ 2.24.25; i586-pc-linux-gnu) 7, 49 -- MIME-Version: 1.0 7, 49 -- Content-Type: text/plain; charset=US-ASCII 7, 49 -- Content-Transfer-Encoding: 7bit 7, 49 -- X-IoP-CAS-MailScanner-ID: 92E1E2C80FA.98AF0 7, 49 -- X-IoP-CAS-MailScanner: Processed 7, 49 -- X-Spam-Status: No 7, 49 -- X-Barracuda-Connect: mail2.biomed.cas.cz[147.231.40.32] 7, 49 -- X-Barracuda-Start-Time: 1452240773 7, 49 -- X-Barracuda-Encrypted: DHE-RSA-AES256-SHA 7, 49 -- X-Barracuda-URL: https://barracuda.biomed.cas.cz:443/cgi-mod/mark.cgi 7, 49 -- X-Barracuda-Scan-Msg-Size: 3452 7, 49 -- X-Virus-Scanned: by bsmtpd at biomed.cas.cz 7, 49 -- X-Barracuda-BRTS-Status: 1 7, 49 -- X-Barracuda-Spam-Score: 0.00 7, 49 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using per-user scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=9.0 tests=BSF_SC0_MISMATCH_TO 7, 49 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.25946 7, 49 -- Rule breakdown below 7, 49 -- pts rule name description 7, 49 -- ---- ---------------------- -------------------------------------------------- 7, 49 -- 0.00 BSF_SC0_MISMATCH_TO Envelope rcpt doesn't match header ==============================End of - Headers==============================
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Email: gary.bloomer-at-montana.edu Name: Gary Bloomer
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Title-Subject: [Filtered] Chromophores characterized for 2-photon microscopy
Message: We are doing 2-photon microscopy but the available chromophores all seem to be characterized only for 1 photon fluorescence. Setting parameters on the light source according to this data may be creating an error in our results. Are others concerned with this and are there solutions such as look-up tables available to properly set laser parameters for available dyes?
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==============================Original Headers============================== 12, 40 -- From microscopy.listserver-at-gmail.com Fri Jan 8 07:44:15 2016 12, 40 -- Received: from mail-io0-f176.google.com (mail-io0-f176.google.com [209.85.223.176]) 12, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u08DiDcm012228 12, 40 -- for {microscopy-at-microscopy.com} ; Fri, 8 Jan 2016 07:44:14 -0600 12, 40 -- Received: by mail-io0-f176.google.com with SMTP id 77so255214254ioc.2 12, 40 -- for {microscopy-at-microscopy.com} ; Fri, 08 Jan 2016 05:44:04 -0800 (PST) 12, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 12, 40 -- d=gmail.com; s=20120113; 12, 40 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 12, 40 -- :mime-version:in-reply-to:content-type:content-transfer-encoding; 12, 40 -- bh=m38CZ0tYTKgidhCo3hLR/qY8vTCi1OBYk0yJJ/0HiBM=; 12, 40 -- b=DztjP7xQD90BIdzGMR5c3w2b2nq61+L2m9OHkC2SnoX9RtmgHQtW0QjunDQhR2E6X/ 12, 40 -- nSCEPDH1lcce7uA2sgDt1XrFHCpKSCh5q89dluFOGf3D1l0GAxstfPjtMRSraGBn1xik 12, 40 -- WooLx9ou6aEIIFAzH9gq2VtV77ZzRxbP5pq9FJ6Zi9VDiL6oTq8BsW3HNghOq8Bcbtja 12, 40 -- au1EvO6ra0j35HTwo56DXdGcesCOr1PGmEgCpjVQM/6fSnOAc4zIdLVbuPUPJtOLcKEo 12, 40 -- vSjz9n1uAtttqHUZCJ6Nuev52eOhKfpSf/kuI76RDZGM8DcW6nTShh+ixBFWs2DrIryJ 12, 40 -- HwYQ== 12, 40 -- X-Received: by 10.107.9.224 with SMTP id 93mr97073491ioj.112.1452260644386; 12, 40 -- Fri, 08 Jan 2016 05:44:04 -0800 (PST) 12, 40 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24]) 12, 40 -- by smtp.googlemail.com with ESMTPSA id n6sm6735362ige.12.2016.01.08.05.44.03 12, 40 -- for {microscopy-at-microscopy.com} 12, 40 -- (version=TLSv1/SSLv3 cipher=OTHER); 12, 40 -- Fri, 08 Jan 2016 05:44:03 -0800 (PST) 12, 40 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 12, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply 12, 40 -- {microscopylistserver-noreply-at-microscopy.com} 12, 40 -- Subject: viaWWW:Chromophores characterized for 2-photon microscopy 12, 40 -- References: {201601072301.u07N1pve032564-at-ns.microscopy.com} 12, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 12, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 12, 40 -- X-Forwarded-Message-Id: {201601072301.u07N1pve032564-at-ns.microscopy.com} 12, 40 -- Message-ID: {568FBD23.1090507-at-microscopy.com} 12, 40 -- Date: Fri, 8 Jan 2016 07:44:03 -0600 12, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0) 12, 40 -- Gecko/20100101 Thunderbird/38.5.0 12, 40 -- MIME-Version: 1.0 12, 40 -- In-Reply-To: {201601072301.u07N1pve032564-at-ns.microscopy.com} 12, 40 -- Content-Type: text/plain; charset=windows-1252; format=flowed 12, 40 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
From hanja07656515151viu-at-gmail.com Fri Jan 8 08:09:14 2016 Return-Path: {hanja07656515151viu-at-gmail.com} Received: from gmail.com (220-134-188-200.HINET-IP.hinet.net [220.134.188.200]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id u08E9BX8030857 for {microscopylistserverarchive5-at-microscopy.com} ; Fri, 8 Jan 2016 08:09:13 -0600 Message-ID: {C2B18D2B.24421114-at-gmail.com}
Dear Listers,
I'm currently fixing a strain of E. Coli with pili with 2%GA , 2%Paraform. In .1M Sorensen's phosphate buffer. Filtering them through a small Millipore filter and then processing this filter disc in the usual way up through CPD, mounting, sputter coating with gold/palladium for SEM. Most of the pili seem to have fallen off the bacteria, plenty of loose pili on the filter beside the bacteria.
Couple of questions for everybody. First, can fixation cause pili to shear off the bacteria? Second, anyone have any suggestions on how to keep the pili attached to the bacteria for SEM observation? Thanks in advance, all help is appreciated.
Tom Bargar UNMC Electron Microscopy Core Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
The information in this e-mail may be privileged and confidential, intended only for the use of the addressee(s) above. Any unauthorized use or disclosure of this information is prohibited. If you have received this e-mail by mistake, please delete it and immediately contact the sender.
==============================Original Headers============================== 8, 37 -- From tbargar-at-unmc.edu Fri Jan 8 08:26:56 2016 8, 37 -- Received: from zixvpm01.unmc.edu (zixvpm01.unmc.edu [192.198.54.126]) 8, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u08EQuO4003928 8, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 8 Jan 2016 08:26:56 -0600 8, 37 -- Received: from 127.0.0.1 (ZixVPM [127.0.0.1]) 8, 37 -- by Outbound.unmc.edu (Proprietary) with SMTP id B5B455C3BF6 8, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 8 Jan 2016 08:07:20 -0600 (CST) 8, 37 -- Received: from UNMCEX1.unmcresforest.org (unknown [10.8.3.1]) 8, 37 -- (using TLSv1 with cipher AES128-SHA (128/128 bits)) 8, 37 -- (No client certificate requested) 8, 37 -- by zixvpm01.unmc.edu (Proprietary) with ESMTPS id 070435C3B2D 8, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 8 Jan 2016 08:07:20 -0600 (CST) 8, 37 -- Received: from UNMCEX2.unmcresforest.org ([fe80::151d:89d:49b6:a0ab]) by 8, 37 -- UNMCEX1.unmcresforest.org ([fe80::2023:ee90:3fae:f0d6%12]) with mapi id 8, 37 -- 14.03.0235.001; Fri, 8 Jan 2016 08:26:45 -0600 8, 37 -- From: "Bargar, Tom W" {tbargar-at-unmc.edu} 8, 37 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 8, 37 -- Subject: Stabilizing pili on bacteria for SEM 8, 37 -- Thread-Topic: Stabilizing pili on bacteria for SEM 8, 37 -- Thread-Index: AdFKH6WOLCX89yPzR5e9+rDjaEY6nA== 8, 37 -- Date: Fri, 8 Jan 2016 14:26:44 +0000 8, 37 -- Message-ID: {E5858BD0583CA8499131AC34AD6766B0AE84D685-at-UNMCEX2.unmcresforest.org} 8, 37 -- Accept-Language: en-US 8, 37 -- Content-Language: en-US 8, 37 -- X-MS-Has-Attach: 8, 37 -- X-MS-TNEF-Correlator: 8, 37 -- x-originating-ip: [10.8.64.15] 8, 37 -- Content-Type: text/plain; charset="us-ascii" 8, 37 -- MIME-Version: 1.0 8, 37 -- X-VPM-MSG-ID: 86ea5b32-28f1-4bca-b83d-6df6163209ce 8, 37 -- X-VPM-HOST: zixvpm01.unmc.edu 8, 37 -- X-VPM-GROUP-ID: f91a6e30-52f8-4523-846a-55fd5677fb4d 8, 37 -- X-VPM-ENC-REGIME: ZixVPM,Plaintext 8, 37 -- X-VPM-CERT-FLAG: 0 8, 37 -- X-VPM-IS-HYBRID: 0 8, 37 -- Content-Transfer-Encoding: 8bit 8, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u08EQuO4003928 ==============================End of - Headers==============================
From advertise.bz222uuv-at-gmail.com Fri Jan 8 09:32:23 2016 Return-Path: {advertise.bz222uuv-at-gmail.com} Received: from gmail.com ([1.236.84.191]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id u08FWKsx027065 for {microscopylistserverarchive5-at-microscopy.com} ; Fri, 8 Jan 2016 09:32:21 -0600 Message-ID: {62EBFA9A.D09DB7CA-at-gmail.com}
Tom, Are you following the primary fixative with osmium? Does this strain have pili? You can run a quick negative stain prep to confirm pili. Also maybe some tannic acid in the primary fixative along with PF and Ga. I never heard of pili falling of during fixation.
Good luck, Michael Delannoy
-----Original Message----- X-from: tbargar-at-unmc.edu [mailto:tbargar-at-unmc.edu] Sent: Friday, January 08, 2016 9:38 AM To: delannoy-at-jhmi.edu
Dear Listers,
I'm currently fixing a strain of E. Coli with pili with 2%GA , 2%Paraform. In .1M Sorensen's phosphate buffer. Filtering them through a small Millipore filter and then processing this filter disc in the usual way up through CPD, mounting, sputter coating with gold/palladium for SEM. Most of the pili seem to have fallen off the bacteria, plenty of loose pili on the filter beside the bacteria.
Couple of questions for everybody. First, can fixation cause pili to shear off the bacteria? Second, anyone have any suggestions on how to keep the pili attached to the bacteria for SEM observation? Thanks in advance, all help is appreciated.
Tom Bargar UNMC Electron Microscopy Core Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
The information in this e-mail may be privileged and confidential, intended only for the use of the addressee(s) above. Any unauthorized use or disclosure of this information is prohibited. If you have received this e-mail by mistake, please delete it and immediately contact the sender.
==============================Original Headers============================== 8, 37 -- From tbargar-at-unmc.edu Fri Jan 8 08:26:56 2016 8, 37 -- Received: from zixvpm01.unmc.edu (zixvpm01.unmc.edu [192.198.54.126]) 8, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u08EQuO4003928 8, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 8 Jan 2016 08:26:56 -0600 8, 37 -- Received: from 127.0.0.1 (ZixVPM [127.0.0.1]) 8, 37 -- by Outbound.unmc.edu (Proprietary) with SMTP id B5B455C3BF6 8, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 8 Jan 2016 08:07:20 -0600 (CST) 8, 37 -- Received: from UNMCEX1.unmcresforest.org (unknown [10.8.3.1]) 8, 37 -- (using TLSv1 with cipher AES128-SHA (128/128 bits)) 8, 37 -- (No client certificate requested) 8, 37 -- by zixvpm01.unmc.edu (Proprietary) with ESMTPS id 070435C3B2D 8, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 8 Jan 2016 08:07:20 -0600 (CST) 8, 37 -- Received: from UNMCEX2.unmcresforest.org ([fe80::151d:89d:49b6:a0ab]) by 8, 37 -- UNMCEX1.unmcresforest.org ([fe80::2023:ee90:3fae:f0d6%12]) with mapi id 8, 37 -- 14.03.0235.001; Fri, 8 Jan 2016 08:26:45 -0600 8, 37 -- From: "Bargar, Tom W" {tbargar-at-unmc.edu} 8, 37 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 8, 37 -- Subject: Stabilizing pili on bacteria for SEM 8, 37 -- Thread-Topic: Stabilizing pili on bacteria for SEM 8, 37 -- Thread-Index: AdFKH6WOLCX89yPzR5e9+rDjaEY6nA== 8, 37 -- Date: Fri, 8 Jan 2016 14:26:44 +0000 8, 37 -- Message-ID: {E5858BD0583CA8499131AC34AD6766B0AE84D685-at-UNMCEX2.unmcresforest.org} 8, 37 -- Accept-Language: en-US 8, 37 -- Content-Language: en-US 8, 37 -- X-MS-Has-Attach: 8, 37 -- X-MS-TNEF-Correlator: 8, 37 -- x-originating-ip: [10.8.64.15] 8, 37 -- Content-Type: text/plain; charset="us-ascii" 8, 37 -- MIME-Version: 1.0 8, 37 -- X-VPM-MSG-ID: 86ea5b32-28f1-4bca-b83d-6df6163209ce 8, 37 -- X-VPM-HOST: zixvpm01.unmc.edu 8, 37 -- X-VPM-GROUP-ID: f91a6e30-52f8-4523-846a-55fd5677fb4d 8, 37 -- X-VPM-ENC-REGIME: ZixVPM,Plaintext 8, 37 -- X-VPM-CERT-FLAG: 0 8, 37 -- X-VPM-IS-HYBRID: 0 8, 37 -- Content-Transfer-Encoding: 8bit 8, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u08EQuO4003928 ==============================End of - Headers==============================
==============================Original Headers============================== 16, 24 -- From prvs=808477f31=mdelann1-at-jhmi.edu Fri Jan 8 10:38:14 2016 16, 24 -- Received: from smtpauth.johnshopkins.edu (smtpauth.johnshopkins.edu [162.129.8.130]) 16, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u08GcE5W027977 16, 24 -- for {Microscopy-at-microscopy.com} ; Fri, 8 Jan 2016 10:38:14 -0600 16, 24 -- X-IronPort-AV: E=Sophos;i="5.20,539,1444708800"; 16, 24 -- d="scan'208";a="204135271" 16, 24 -- Received: from unknown (HELO BSNO8925) ([10.16.66.96]) 16, 24 -- by IPEB1.johnshopkins.edu with ESMTP/TLS/AES256-SHA; 08 Jan 2016 11:38:03 -0500 16, 24 -- From: "Michael Delannoy" {mdelann1-at-jhmi.edu} 16, 24 -- To: {tbargar-at-unmc.edu} 16, 24 -- Cc: {Microscopy-at-microscopy.com} , {microscopy.listserver-at-gmail.com} , 16, 24 -- {microscopy-at-msa.microscopy.com} 16, 24 -- References: {201601081437.u08EbZvM010463-at-ns.microscopy.com} 16, 24 -- In-Reply-To: {201601081437.u08EbZvM010463-at-ns.microscopy.com} 16, 24 -- Subject: RE: [Microscopy] Stabilizing pili on bacteria for SEM 16, 24 -- Date: Fri, 8 Jan 2016 11:38:06 -0500 16, 24 -- Message-ID: {007b01d14a32$f3daad70$db900850$-at-jhmi.edu} 16, 24 -- MIME-Version: 1.0 16, 24 -- Content-Type: text/plain; 16, 24 -- charset="us-ascii" 16, 24 -- Content-Transfer-Encoding: 7bit 16, 24 -- X-Mailer: Microsoft Outlook 14.0 16, 24 -- Thread-Index: AQKcnZohv6HMCmg8szDXNpP2BC84M51bKVGg 16, 24 -- Content-Language: en-us ==============================End of - Headers==============================
Hello Tom, We are using E. coli in our practical courses of electron microscopy in biology. We usually process E. coli samples for TEM and SEM in parallel.
At first, do you really need a SEM for imaging E. coli pili? TEM and negative staining is much more faster a you even do not need to fix E. coli sample at all. In our hand 1% ammonium molybdate, pH6.8-7.0 plus 0.1% trehalose mixture works quite well for negative staining of bacteria on glow-discharge treated formvar/carbon grids.
Our procedure for SEM: 1. Start with 0.5 ml of E. coli culture; optical density ranging from 0.4 to 0.6. 2. Fix it with 3% glutaraldehyde for half an hour at room temperature, then continue the fixation at 4 oC overnight. 3. Wash the fixed cells well with phosphate or cacodylate buffer (three times at least). 4. Make a moist chamber from Petri dish, wet filter paper and Parafilm. 5. Place some poly-l-lysine treated circular cover-slips on the Parafilm in the moist chamber and put 100 ,Aei 200 uL of fixed cells in the washing buffer onto the individual cover-slip. Whole cover-slip area should be covered with liquid. Let sediment the cells onto the cover-slips at 4 oC overnight. 6. Wash the cover-slips with ddH2O. 7. Dehydrate through alcohol series; CPD; ... .
With this procedure the pili should stay attached to bacteria.
Best regards Oldrich
-- Oldrich Benada Institute of Microbiology CAS, v.v.i. Videnska 1083 142 20 Prague 4 Czech Republic
On Fri, 8 Jan 2016 08:31:14 -0600, tbargar-at-unmc.edu wrote : } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Listers, } } I'm currently fixing a strain of E. Coli with pili with 2%GA , } 2%Paraform. In .1M Sorensen's phosphate buffer. Filtering them } through a small Millipore filter and then processing this filter disc } in the usual way up through CPD, mounting, sputter coating with } gold/palladium for SEM. Most of the pili seem to have fallen off the } bacteria, plenty of loose pili on the filter beside the bacteria. } } Couple of questions for everybody. First, can fixation cause pili to } shear off the bacteria? Second, anyone have any suggestions on how } to keep the pili attached to the bacteria for SEM observation? } Thanks in advance, all help is appreciated. } } Tom Bargar } UNMC } Electron Microscopy Core Facility } 986395 Nebraska Medical Center } Omaha, NE 68198-6395 } 402-559-7347 } tbargar-at-unmc.edu } } } The information in this e-mail may be privileged and confidential, } intended only for the use of the addressee(s) above. Any unauthorized } use or disclosure of this information is prohibited. If you have } received this e-mail by mistake, please delete it and immediately } contact the sender. } } } } ==============================Original } Headers============================== 8, 37 -- From tbargar-at-unmc.edu } Fri Jan 8 08:26:56 2016 8, 37 -- Received: from zixvpm01.unmc.edu } (zixvpm01.unmc.edu [192.198.54.126]) 8, 37 -- by } ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } u08EQuO4003928 8, 37 -- for {Microscopy-at-microscopy.com} ; Fri, } 8 Jan 2016 08:26:56 -0600 8, 37 -- Received: from 127.0.0.1 (ZixVPM } [127.0.0.1]) 8, 37 -- by Outbound.unmc.edu (Proprietary) with
Virginia Tech in Blacksburg, VA, USA has an opening for an Applications Specialist in Scanning and Transmission Electron Microscopy. This position will help support internal and external users of the Virginia Tech National Center for Earth and Environmental Nanotechnology Infrastructure (NCE- {=NI) under the direct supervision of NCE- {=NI Associate Director for Instrumentation & Technical Development. The successful candidate will have their office located within the Nanoscale Characterization and Fabrication Laboratory (NCFL). The NCFL houses advanced field emission scanning electron microscopes with EDS and EBSD capabilities, several transmission electron microscopes with EDS, tomography, HRTEM, cryoTEM, and other capabilities, and a suite of powerful surface spectroscopy tools including magnetic sector SIMS, XPS, and more. Multiple sample preparation laboratories facilitate the preparation of samples for analysis using SEM, TEM, AFM, EBSD, and other techniques.
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To view a more detailed list of expected duties and the qualifications required, visit:
https://listings.jobs.vt.edu/postings/62637
Applicants can apply to this position by visiting the above link and clicking on the "Apply for this Job" link near the top of the page. If the above link does not work, then you can apply by visiting www.jobs.vt.edu and searching for posting number SR0150183.
Virginia Tech does not discriminate against employees, students, or applicants on the basis of age, color, disability, gender, gender identity, gender expression, national origin, political affiliation, race, religion, sexual orientation, genetic information, veteran status, or any other basis protected by law. For inquiries regarding non-discrimination policies, contact the executive director for Equity and Access at 540-231-8771 or Virginia Tech, North End Center, Suite 2300 (0318), 300 Turner St. NW, Blacksburg, VA 24061.
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From improve.alexa.rankslsuzn-at-gmail.com Sat Jan 9 13:10:08 2016 Return-Path: {improve.alexa.rankslsuzn-at-gmail.com} Received: from gmail.com ([211.63.33.142]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id u09JA2g5002263 for {microscopylistserverarchive5-at-microscopy.com} ; Sat, 9 Jan 2016 13:10:05 -0600 Message-ID: {F8A49867.6949C4DE-at-gmail.com}
Dear Microscopists,
We have an entry level opening in our Tucson manufacturing facility suitable for an enthusiastic energetic recent graduate with good communication skills, to join us as a full time EM tech in our applications support lab.
Initial duties include:-
* Ultramicrotome QC - cut ultra thin sections & evaluate in TEM + basic QC evaluation of sub assemblies/components + submit reports & documentation * Glass Knife Maker test, calibration and QC reports * Rotary microtome QC - cut semi-thin to thick paraffin and materials science samples - evaluate & report * TEM maintenance
with opportunities for growth in the following areas:
* Applications support evaluating customer specimens and advising on appropriate sample prep techniques - includes both life science and materials science disciplines * Cryo Ultramicrotome test and evaluation * Assist with workshops and product demonstrations * Field support if applicant is willing and able to travel * Sales and Service
Please respond by email to info-at-boeckeler.com
-- Dave Roberts Director-RMC Microscopy Products RMC-Boeckeler Boeckeler Instruments Inc 4650 S Butterfield Drive Tucson, Arizona 85714 Tel: 520-745-0001 Fax: 520-745-0004 www.rmcproducts.com Skype:dave.robertsrmc
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Someone has asked me about microscope attachments for iPhone. I've seen lots of ads, but I haven't looked closely at any of the products. Do any of you have opinions about products that turn your iPhone or Android into decent microscopes? I'm not sure if she wants to go with a stand and slides, or just take nicely magnified images on the go. I think the former...
Aloha, Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 4, 21 -- From tina-at-pbrc.hawaii.edu Wed Jan 13 12:31:52 2016 4, 21 -- Received: from b1000.pbrc.hawaii.edu (b1000.pbrc.hawaii.edu [128.171.22.30]) 4, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0DIVppj017621 4, 21 -- for {Microscopy-at-microscopy.com} ; Wed, 13 Jan 2016 12:31:52 -0600 4, 21 -- Received: from b1000.pbrc.hawaii.edu (localhost [127.0.0.1]) 4, 21 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id u0DIVVV9025775 4, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 4, 21 -- Wed, 13 Jan 2016 08:31:31 -1000 (HST) 4, 21 -- Received: from localhost (tina-at-localhost) 4, 21 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP id u0DIVUQ2025771; 4, 21 -- Wed, 13 Jan 2016 08:31:30 -1000 (HST) 4, 21 -- X-Authentication-Warning: b1000.pbrc.hawaii.edu: tina owned process doing -bs 4, 21 -- Date: Wed, 13 Jan 2016 08:31:30 -1000 (HST) 4, 21 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 4, 21 -- X-X-Sender: tina-at-b1000 4, 21 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} , 4, 21 -- confocalmicroscopy-at-LISTS.UMN.EDU 4, 21 -- Subject: iPhone microscope 4, 21 -- Message-ID: {Pine.GSO.4.64.1601130826590.25665-at-b1000} 4, 21 -- MIME-Version: 1.0 4, 21 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
Here at PNNL we,Aeove actually developed a low-cost (~$1) cellphone camera that magnifies up to 1000x, depending on the specification. Our lab has freely released the blueprints for 3D printing or you can order pre-manufactured lenses that clip onto pretty much any smartphone. We use them frequently in our demos to high school and middle school children and they work quite well once you get the hang of them.
Here,Aeos a link to more details: http://availabletechnologies.pnnl.gov/technology.asp?id=393
Feel free to contact me if you have any questions.
* The views and opinions expressed in this email are my own and do not necessarily reflect those of PNNL, the Battelle Memorial Institute, the United States Government, or any agency thereof. ______________________________________ Steven R. Spurgeon, Ph.D. Postdoctoral Research Associate Physical and Computational Sciences Directorate
Pacific Northwest National Laboratory 902 Battelle Boulevard P.O. Box 999 MSIN:K8-87 Richland, WA 99352
Someone has asked me about microscope attachments for iPhone. I've seen lots of ads, but I haven't looked closely at any of the products. Do any of you have opinions about products that turn your iPhone or Android into decent microscopes? I'm not sure if she wants to go with a stand and slides, or just take nicely magnified images on the go. I think the former...
Aloha, Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 4, 21 -- From tina-at-pbrc.hawaii.edu Wed Jan 13 12:31:52 2016 4, 21 -- Received: from b1000.pbrc.hawaii.edu (b1000.pbrc.hawaii.edu [128.171.22.30]) 4, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0DIVppj017621 4, 21 -- for {Microscopy-at-microscopy.com} ; Wed, 13 Jan 2016 12:31:52 -0600 4, 21 -- Received: from b1000.pbrc.hawaii.edu (localhost [127.0.0.1]) 4, 21 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id u0DIVVV9025775 4, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 4, 21 -- Wed, 13 Jan 2016 08:31:31 -1000 (HST) 4, 21 -- Received: from localhost (tina-at-localhost) 4, 21 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP id u0DIVUQ2025771; 4, 21 -- Wed, 13 Jan 2016 08:31:30 -1000 (HST) 4, 21 -- X-Authentication-Warning: b1000.pbrc.hawaii.edu: tina owned process doing -bs 4, 21 -- Date: Wed, 13 Jan 2016 08:31:30 -1000 (HST) 4, 21 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 4, 21 -- X-X-Sender: tina-at-b1000 4, 21 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} , 4, 21 -- confocalmicroscopy-at-LISTS.UMN.EDU 4, 21 -- Subject: iPhone microscope 4, 21 -- Message-ID: {Pine.GSO.4.64.1601130826590.25665-at-b1000} 4, 21 -- MIME-Version: 1.0 4, 21 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
==============================Original Headers============================== 22, 29 -- From prvs=813e0a7d3=steven.spurgeon-at-pnnl.gov Wed Jan 13 13:11:57 2016 22, 29 -- Received: from emailgw02.pnnl.gov (emailgw02.pnnl.gov [192.101.109.63]) 22, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0DJBuxL006200 22, 29 -- for {Microscopy-at-microscopy.com} ; Wed, 13 Jan 2016 13:11:56 -0600 22, 29 -- Received: from ex10cashub06.pnnl.gov ([130.20.248.212]) 22, 29 -- by emailgw02.pnnl.gov with ESMTP/TLS/AES256-SHA; 13 Jan 2016 11:11:45 -0800 22, 29 -- Received: from EX10MBOX03.pnnl.gov ([169.254.3.82]) by EX10CASHUB06.pnnl.gov 22, 29 -- ([130.20.248.212]) with mapi id 14.03.0248.002; Wed, 13 Jan 2016 11:11:45 22, 29 -- -0800 22, 29 -- From: "Spurgeon, Steven R" {steven.spurgeon-at-pnnl.gov} 22, 29 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} , 22, 29 -- "tina-at-pbrc.hawaii.edu" {tina-at-pbrc.hawaii.edu} 22, 29 -- Subject: Re: [Microscopy] iPhone microscope 22, 29 -- Thread-Topic: [Microscopy] iPhone microscope 22, 29 -- Thread-Index: AQHRTjQyIKPRV14ru0iCfvZUlj7Hu5750BIA 22, 29 -- Date: Wed, 13 Jan 2016 19:11:44 +0000 22, 29 -- Message-ID: {062CD028-BFB5-47EE-A028-A5F9044AB27E-at-pnnl.gov} 22, 29 -- References: {201601131856.u0DIudAw004154-at-ns.microscopy.com} 22, 29 -- In-Reply-To: {201601131856.u0DIudAw004154-at-ns.microscopy.com} 22, 29 -- Accept-Language: en-US 22, 29 -- Content-Language: en-US 22, 29 -- X-MS-Has-Attach: 22, 29 -- X-MS-TNEF-Correlator: 22, 29 -- x-originating-ip: [130.20.128.10] 22, 29 -- Content-Type: text/plain; charset="utf-8" 22, 29 -- Content-ID: {D94556B5D077EF4595F2908D97115031-at-pnnl.gov} 22, 29 -- MIME-Version: 1.0 22, 29 -- Content-Transfer-Encoding: 8bit 22, 29 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id u0DJBuxL006200 ==============================End of - Headers==============================
Hi Tina, Though not as cheap as the PNNL design, some colleagues of mine have used this inexpensive, hardware-store-materials-based home-built design for outreach and liked it: http://www.instructables.com/id/10-Smartphone-to-digital-microscope-conversion/ Tyler Harvey University of Oregon
On 2016-01-13 11:29, steven.spurgeon-at-pnnl.gov wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello Tina, } } Here at PNNL we/c,C ,Ncve actually developed a low-cost (~$1) cellphone } camera that magnifies up to 1000x, depending on the specification. Our } lab has freely released the blueprints for 3D printing or you can } order pre-manufactured lenses that clip onto pretty much any } smartphone. We use them frequently in our demos to high school and } middle school children and they work quite well once you get the hang } of them. } } Here/c,C ,Ncs a link to more details: } http://availabletechnologies.pnnl.gov/technology.asp?id=393 } } Feel free to contact me if you have any questions. } } * The views and opinions expressed in this email are my own and do not } necessarily reflect those of PNNL, the Battelle Memorial Institute, } the United States Government, or any agency thereof. } ______________________________________ } Steven R. Spurgeon, Ph.D. } Postdoctoral Research Associate } Physical and Computational Sciences Directorate } } Pacific Northwest National Laboratory } 902 Battelle Boulevard } P.O. Box 999 MSIN:K8-87 } Richland, WA 99352 } } Tel: +1-509-371-7123 } steven.spurgeon-at-pnnl.gov } www.stevenspurgeon.com {http://www.stevenspurgeon.com/} } www.pnnl.gov {http://www.pnnl.gov/} } } } } } X-from: "tina-at-pbrc.hawaii.edu" {tina-at-pbrc.hawaii.edu} } Reply-To: "tina-at-pbrc.hawaii.edu" {tina-at-pbrc.hawaii.edu} } Date: Wednesday, January 13, 2016 at 10:56 AM } To: Steven Spurgeon {steven.spurgeon-at-pnnl.gov} } Subject: [Microscopy] iPhone microscope } } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } My apologies for cross-posting... } } Someone has asked me about microscope attachments for iPhone. I've seen } lots of ads, but I haven't looked closely at any of the products. Do } any } of you have opinions about products that turn your iPhone or Android } into } decent microscopes? I'm not sure if she wants to go with a stand and } slides, or just take nicely magnified images on the go. I think the } former... } } Aloha, } Tina } } **************************************************************************** } * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu } * } * Biological Electron Microscope Facility * (808) 956-6251 } * } * University of Hawaii at Manoa * } http://www.pbrc.hawaii.edu/bemf* } **************************************************************************** } } ==============================Original } Headers============================== } 4, 21 -- From tina-at-pbrc.hawaii.edu Wed Jan 13 12:31:52 2016 } 4, 21 -- Received: from b1000.pbrc.hawaii.edu (b1000.pbrc.hawaii.edu } [128.171.22.30]) } 4, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } u0DIVppj017621 } 4, 21 -- for {Microscopy-at-microscopy.com} ; Wed, 13 Jan 2016 12:31:52 } -0600 } 4, 21 -- Received: from b1000.pbrc.hawaii.edu (localhost [127.0.0.1]) } 4, 21 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id } u0DIVVV9025775 } 4, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 } verify=NO); } 4, 21 -- Wed, 13 Jan 2016 08:31:31 -1000 (HST) } 4, 21 -- Received: from localhost (tina-at-localhost) } 4, 21 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP } id u0DIVUQ2025771; } 4, 21 -- Wed, 13 Jan 2016 08:31:30 -1000 (HST) } 4, 21 -- X-Authentication-Warning: b1000.pbrc.hawaii.edu: tina owned } process doing -bs } 4, 21 -- Date: Wed, 13 Jan 2016 08:31:30 -1000 (HST) } 4, 21 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} } 4, 21 -- X-X-Sender: tina-at-b1000 } 4, 21 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} , } 4, 21 -- confocalmicroscopy-at-LISTS.UMN.EDU } 4, 21 -- Subject: iPhone microscope } 4, 21 -- Message-ID: {Pine.GSO.4.64.1601130826590.25665-at-b1000} } 4, 21 -- MIME-Version: 1.0 } 4, 21 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed } ==============================End of - } Headers============================== } } } } ==============================Original } Headers============================== } 22, 29 -- From prvs=813e0a7d3=steven.spurgeon-at-pnnl.gov Wed Jan 13 } 13:11:57 2016 } 22, 29 -- Received: from emailgw02.pnnl.gov (emailgw02.pnnl.gov } [192.101.109.63]) } 22, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id u0DJBuxL006200 } 22, 29 -- for {Microscopy-at-microscopy.com} ; Wed, 13 Jan 2016 13:11:56 } -0600 } 22, 29 -- Received: from ex10cashub06.pnnl.gov ([130.20.248.212]) } 22, 29 -- by emailgw02.pnnl.gov with ESMTP/TLS/AES256-SHA; 13 Jan } 2016 11:11:45 -0800 } 22, 29 -- Received: from EX10MBOX03.pnnl.gov ([169.254.3.82]) by } EX10CASHUB06.pnnl.gov } 22, 29 -- ([130.20.248.212]) with mapi id 14.03.0248.002; Wed, 13 Jan } 2016 11:11:45 } 22, 29 -- -0800 } 22, 29 -- From: "Spurgeon, Steven R" {steven.spurgeon-at-pnnl.gov} } 22, 29 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} , } 22, 29 -- "tina-at-pbrc.hawaii.edu" {tina-at-pbrc.hawaii.edu} } 22, 29 -- Subject: Re: [Microscopy] iPhone microscope } 22, 29 -- Thread-Topic: [Microscopy] iPhone microscope } 22, 29 -- Thread-Index: AQHRTjQyIKPRV14ru0iCfvZUlj7Hu5750BIA } 22, 29 -- Date: Wed, 13 Jan 2016 19:11:44 +0000 } 22, 29 -- Message-ID: {062CD028-BFB5-47EE-A028-A5F9044AB27E-at-pnnl.gov} } 22, 29 -- References: {201601131856.u0DIudAw004154-at-ns.microscopy.com} } 22, 29 -- In-Reply-To: {201601131856.u0DIudAw004154-at-ns.microscopy.com} } 22, 29 -- Accept-Language: en-US } 22, 29 -- Content-Language: en-US } 22, 29 -- X-MS-Has-Attach: } 22, 29 -- X-MS-TNEF-Correlator: } 22, 29 -- x-originating-ip: [130.20.128.10] } 22, 29 -- Content-Type: text/plain; charset="utf-8" } 22, 29 -- Content-ID: {D94556B5D077EF4595F2908D97115031-at-pnnl.gov} } 22, 29 -- MIME-Version: 1.0 } 22, 29 -- Content-Transfer-Encoding: 8bit } 22, 29 -- X-MIME-Autoconverted: from base64 to 8bit by } ns.microscopy.com id u0DJBuxL006200 } ==============================End of - } Headers==============================
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Email: Phil.Ahrenkiel-at-sdsmt.edu Name: Phil Ahrenkiel
Organization: SDSMT
Title-Subject: [Filtered] Orius column defect
Message: Does anybody know how to use the "Defects" box in the "Camera Configuration" dialog in DM for an Orius? I have spent several hours getting remote help from Gatan, but nobody is able to make that feature actually work. The format seems to be "c2098", for column 2098. We are using DM1.85.1535.
The details of our problem are shown here: http://ahrenkiel.sdsmt.edu/JEM2100_TEM/troubleshooting/default.htm
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Title-Subject: [Filtered] Liposome in skin tissue - Ultrastructure by TEM
Message: Does anyone worked on visualizing liposomes in skin tissue by TEM. Is there any special precautions/steps to consider for processing the tissue.?
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Pili are extremely delicate, I would guess that the filtration step is the critical one in your protocol because it requires pressure. In this regard, the protocol of Oldrich is much softer since the cells are sedimented. We placed bacteria on a porous membrane (used for cell biology) and sucked the liquid from the bottom side with a filter. The filters give charging in SEM and they tend to melt under the beam, so be careful with your electrons! (some years ago we had porous membranes made of aluminium, they were perfect for the job but I think they are not produced anymore)
Regards Stephane
-------------------------------------------- On Fri, 1/8/16, benada-at-biomed.cas.cz {benada-at-biomed.cas.cz} wrote:
Subject: [Microscopy] Re: Stabilizing pili on bacteria for SEM To: nizets2-at-yahoo.com Date: Friday, January 8, 2016, 6:13 PM
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Hello Tom, We are using E. coli in our practical courses of electron microscopy in biology. We usually process E. coli samples for TEM and SEM in parallel.
At first, do you really need a SEM for imaging E. coli pili? TEM and negative staining is much more faster a you even do not need to fix E. coli sample at all. In our hand 1% ammonium molybdate, pH6.8-7.0 plus 0.1% trehalose mixture works quite well for negative staining of bacteria on glow-discharge treated formvar/carbon grids.
Our procedure for SEM: 1. Start with 0.5 ml of E. coli culture; optical density ranging from 0.4 to 0.6. 2. Fix it with 3% glutaraldehyde for half an hour at room temperature, then continue the fixation at 4 oC overnight. 3. Wash the fixed cells well with phosphate or cacodylate buffer (three times at least). 4. Make a moist chamber from Petri dish, wet filter paper and Parafilm. 5. Place some poly-l-lysine treated circular cover-slips on the Parafilm in the moist chamber and put 100 ,Aei 200 uL of fixed cells in the washing buffer onto the individual cover-slip. Whole cover-slip area should be covered with liquid. Let sediment the cells onto the cover-slips at 4 oC overnight. 6. Wash the cover-slips with ddH2O. 7. Dehydrate through alcohol series; CPD; ... .
With this procedure the pili should stay attached to bacteria.
Best regards Oldrich
-- Oldrich Benada Institute of Microbiology CAS, v.v.i. Videnska 1083 142 20 Prague 4 Czech Republic
On Fri, 8 Jan 2016 08:31:14 -0600, tbargar-at-unmc.edu wrote : } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor:-* The Microscopy Society of } America To-* Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Listers, } } I'm currently fixing a strain of E. Coli with pili with 2%GA , } 2%Paraform. In .1M Sorensen's phosphate buffer.-* Filtering them } through a small Millipore filter and then processing this filter disc } in the usual way up through CPD, mounting, sputter coating with } gold/palladium for SEM.-* Most of the pili seem to have fallen off the } bacteria, plenty of loose pili on the filter beside the bacteria. } } Couple of questions for everybody.-* First, can fixation cause pili to } shear off the bacteria?-*-*-*Second, anyone have any suggestions on how } to keep the pili attached to the bacteria for SEM observation? } Thanks in advance, all help is appreciated. } } Tom Bargar } UNMC } Electron Microscopy Core Facility } 986395 Nebraska Medical Center } Omaha, NE 68198-6395 } 402-559-7347 } tbargar-at-unmc.edu } } } The information in this e-mail may be privileged and confidential, } intended only for the use of the addressee(s) above. Any unauthorized } use or disclosure of this information is prohibited. If you have } received this e-mail by mistake, please delete it and immediately } contact the sender. } } } -* } ==============================Original } Headers============================== 8, 37 -- From tbargar-at-unmc.edu } Fri Jan-* 8 08:26:56 2016 8, 37 -- Received: from zixvpm01.unmc.edu } (zixvpm01.unmc.edu [192.198.54.126]) 8, 37 -- -*-*-* by } ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } u08EQuO4003928 8, 37 -- -*-*-* for {Microscopy-at-microscopy.com} ; Fri, } 8 Jan 2016 08:26:56 -0600 8, 37 -- Received: from 127.0.0.1 (ZixVPM } [127.0.0.1]) 8, 37 -- -*-*-* by Outbound.unmc.edu (Proprietary) with } SMTP id B5B455C3BF6 8, 37 -- -*-*-* for {Microscopy-at-microscopy.com} ; } Fri,-* 8 Jan 2016 08:07:20 -0600 (CST) 8, 37 -- Received: from } UNMCEX1.unmcresforest.org (unknown [10.8.3.1]) 8, 37 -- } (using TLSv1 with cipher AES128-SHA (128/128 bits)) 8, 37 -- } (No client certificate requested) 8, 37 -- -*-*-* by } zixvpm01.unmc.edu (Proprietary) with ESMTPS id 070435C3B2D 8, 37 -- } -*-*-* for {Microscopy-at-microscopy.com} ; Fri,-* 8 Jan 2016 08:07:20 } -0600 (CST) 8, 37 -- Received: from UNMCEX2.unmcresforest.org } ([fe80::151d:89d:49b6:a0ab]) by 8, 37 ---* UNMCEX1.unmcresforest.org } ([fe80::2023:ee90:3fae:f0d6%12]) with mapi id 8, 37 -- } 14.03.0235.001; Fri, 8 Jan 2016 08:26:45 -0600 8, 37 -- From: } "Bargar, Tom W" {tbargar-at-unmc.edu} 8, 37 -- To: } "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 8, 37 -- } Subject: Stabilizing pili on bacteria for SEM 8, 37 -- Thread-Topic: } Stabilizing pili on bacteria for SEM 8, 37 -- Thread-Index: } AdFKH6WOLCX89yPzR5e9+rDjaEY6nA== 8, 37 -- Date: Fri, 8 Jan 2016 } 14:26:44 +0000 8, 37 -- Message-ID: } {E5858BD0583CA8499131AC34AD6766B0AE84D685-at-UNMCEX2.unmcresforest.org} } 8, 37 -- Accept-Language: en-US 8, 37 -- Content-Language: en-US 8, } 37 -- X-MS-Has-Attach: 8, 37 -- X-MS-TNEF-Correlator: 8, 37 -- } x-originating-ip: [10.8.64.15] 8, 37 -- Content-Type: text/plain; } charset="us-ascii" 8, 37 -- MIME-Version: 1.0 } 8, 37 -- X-VPM-MSG-ID: 86ea5b32-28f1-4bca-b83d-6df6163209ce } 8, 37 -- X-VPM-HOST: zixvpm01.unmc.edu } 8, 37 -- X-VPM-GROUP-ID: f91a6e30-52f8-4523-846a-55fd5677fb4d } 8, 37 -- X-VPM-ENC-REGIME: ZixVPM,Plaintext } 8, 37 -- X-VPM-CERT-FLAG: 0 } 8, 37 -- X-VPM-IS-HYBRID: 0 } 8, 37 -- Content-Transfer-Encoding: 8bit } 8, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id u08EQuO4003928 ==============================End } of - Headers==============================
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==============================Original Headers============================== 13, 46 -- From benada-at-biomed.cas.cz Fri Jan-* 8 11:05:37 2016 13, 46 -- Received: from barracuda.biomed.cas.cz (barracuda.biomed.cas.cz [147.231.40.11]) 13, 46 -- -*-*-* by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u08H5asd016283 13, 46 -- -*-*-* for {microscopy-at-microscopy.com} ; Fri, 8 Jan 2016 11:05:36 -0600 13, 46 -- X-ASG-Debug-ID: 1452272724-05011e54253651e0001-4CH8be 13, 46 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) by barracuda.biomed.cas.cz with ESMTP id gMTvxbAFFmBOVAmD (version=TLSv1 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); Fri, 08 Jan 2016 18:05:25 +0100 (CET) 13, 46 -- X-Barracuda-Envelope-From: benada-at-biomed.cas.cz 13, 46 -- X-Barracuda-Effective-Source-IP: mail2.biomed.cas.cz[147.231.40.32] 13, 46 -- X-Barracuda-Apparent-Source-IP: 147.231.40.32 13, 46 -- Received: from u117ob02 (c111jn.mbu.cas.cz [147.231.44.12]) 13, 46 -- -*-*-* (using TLSv1 with cipher AES128-SHA (128/128 bits)) 13, 46 -- -*-*-* (No client certificate requested) 13, 46 -- -*-*-* by mail2.biomed.cas.cz (Postfix) with ESMTPSA id 2E2172C8070; 13, 46 -- -*-*-* Fri,-* 8 Jan 2016 18:05:20 +0100 (CET) 13, 46 -- Date: Fri, 8 Jan 2016 18:05:19 +0100 13, 46 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 13, 46 -- To: tbargar-at-unmc.edu, {microscopy-at-microscopy.com} 13, 46 -- Subject: Re: [Microscopy] Stabilizing pili on bacteria for SEM 13, 46 -- Message-ID: {20160108180519.01b05d54-at-u117ob02} 13, 46 -- X-ASG-Orig-Subj: Re: [Microscopy] Stabilizing pili on bacteria for SEM 13, 46 -- In-Reply-To: {201601081431.u08EVEY2006755-at-ns.microscopy.com} 13, 46 -- References: {201601081431.u08EVEY2006755-at-ns.microscopy.com} 13, 46 -- Organization: =?UTF-8?B?TWlrcm9iaW9sb2dpY2vDvSDDunN0YXY=?= AV 13, 46 ---* =?UTF-8?B?xIxS?= 13, 46 -- X-Mailer: Claws Mail 3.11.1 (GTK+ 2.24.25; i586-pc-linux-gnu) 13, 46 -- MIME-Version: 1.0 13, 46 -- Content-Type: text/plain; charset=UTF-8 13, 46 -- X-IoP-CAS-MailScanner-ID: 2E2172C8070.17926 13, 46 -- X-IoP-CAS-MailScanner: Processed 13, 46 -- X-Spam-Status: No 13, 46 -- X-Barracuda-Connect: mail2.biomed.cas.cz[147.231.40.32] 13, 46 -- X-Barracuda-Start-Time: 1452272725 13, 46 -- X-Barracuda-Encrypted: DHE-RSA-AES256-SHA 13, 46 -- X-Barracuda-URL: https://barracuda.biomed.cas.cz:443/cgi-mod/mark.cgi 13, 46 -- X-Barracuda-Scan-Msg-Size: 6554 13, 46 -- X-Virus-Scanned: by bsmtpd at biomed.cas.cz 13, 46 -- X-Barracuda-BRTS-Status: 1 13, 46 -- X-Barracuda-Spam-Score: 0.50 13, 46 -- X-Barracuda-Spam-Status: No, SCORE=0.50 using per-user scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=9.0 tests=BSF_RULE7568M 13, 46 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.25954 13, 46 -- -*-*-* Rule breakdown below 13, 46 -- -*-*-*-*-*pts rule name-* -* -* -* -* -* -* description 13, 46 -- -*-*-* ---- ---------------------- -------------------------------------------------- 13, 46 -- -*-*-* 0.50 BSF_RULE7568M-* -* -* -* -* Custom Rule 7568M 13, 46 -- Content-Transfer-Encoding: 8bit 13, 46 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u08H5asd016283 ==============================End of - Headers==============================
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Hahahaa, you guys are so funny. I got 17 replies to my query about turning an iPhone into a microscope, and no two were the same. And most of them were along the lines of "I saw this one on the Internet...".
If anyone is particularly interested, I can send a list of links or forward some of the responses. Here is the one that the person who requested feedback is looking at (and is not one of the ones any of you mentioned):
} My apologies for cross-posting... } } Someone has asked me about microscope attachments for iPhone. I've seen } lots of ads, but I haven't looked closely at any of the products. Do any of } you have opinions about products that turn your iPhone or Android into } decent microscopes? I'm not sure if she wants to go with a stand and } slides, or just take nicely magnified images on the go. I think the } former... } } Aloha, } Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 7, 21 -- From tina-at-pbrc.hawaii.edu Fri Jan 15 18:20:47 2016 7, 21 -- Received: from b1000.pbrc.hawaii.edu (b1000.pbrc.hawaii.edu [128.171.22.30]) 7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0G0KkRe002113 7, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 15 Jan 2016 18:20:47 -0600 7, 21 -- Received: from b1000.pbrc.hawaii.edu (localhost [127.0.0.1]) 7, 21 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id u0G0KTRt000895 7, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 7, 21 -- Fri, 15 Jan 2016 14:20:29 -1000 (HST) 7, 21 -- Received: from localhost (tina-at-localhost) 7, 21 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP id u0G0KSnV000891; 7, 21 -- Fri, 15 Jan 2016 14:20:28 -1000 (HST) 7, 21 -- X-Authentication-Warning: b1000.pbrc.hawaii.edu: tina owned process doing -bs 7, 21 -- Date: Fri, 15 Jan 2016 14:20:28 -1000 (HST) 7, 21 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 7, 21 -- X-X-Sender: tina-at-b1000 7, 21 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} , 7, 21 -- confocalmicroscopy-at-LISTS.UMN.EDU 7, 21 -- Subject: iPhone microscope 7, 21 -- Message-ID: {Pine.GSO.4.64.1601151415220.26754-at-b1000} 7, 21 -- MIME-Version: 1.0 7, 21 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
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Email: Chrissi.Luede-at-gmail.com Name: Christopher L,demann
Organization: RWTH Aachen, GERMANY
Title-Subject: [Filtered] ETEC Autoscan SEM - looking for service manual
Message: Hello everyone,
I rescently acquired a vintage ETEC Autoscan SEM (rebadged by Siemens) and intend to refurbish it as a hobby project.
The instrument came with a basic user manual, but in order to put the electronics back into operation, I am looking for a more detailed service manual (circuit diagrams, exploded drawings, component names, etc.). I would be very grateful, if anyone could share a digitised version of their documents.
And finally, two technical questions: 1. Is there a list of head sizes for the various hex sockets on the electron optical column? Are they metric or imperial? I found a couple of small screws which seem to require something between 2.5...3mm or 1/8...3/32", nothing I would deem as standard...
2. Would you happen to have any documentation on the diffusion pump? I assume it is a Varian type M2. Is is worthwhile to replace it with a turbo pump? I already have all the necessary equipment and would only need to buy an ASA/CF adapter flange plus a vibration damper.
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Email: SLC6-at-Lehigh.edu Name: Sharon Coe
Organization: Lehigh University
Title-Subject: [Filtered] Lehigh Microscopy School 2016
Message: Now accepting registrations for the 46th annual Lehigh Microscopy School which will be held on the campus of Lehigh University, Bethlehem, PA, June 5-10, 2016. All courses, lecturers, and instrument suppliers will be together for what promises to be a phenomenal week! Course offerings include: SEM and X-ray Microanalysis -i Introduction to SEM and EDS for the New Operator -i Focused Ion Beam Instrumentation and Applications -i Problem Solving: Interpretation and Analysis of SEM/EDS/EBSD Data -i Quantitative X-ray Microanalysis: Problem Solving Using EDS and WDS Techniques -i Scanning Transmission Electron Microscopy: From Fundamentals to Advanced Applications. Register and pay in full by April 15 for an Early Bird Discount! Contact: Sharon Coe (sharon.coe-at-Lehigh.edu or 610-758-5133). See www.lehigh.edu/microscopy for registration form, prices, and details about courses, lecturers, and instrument suppliers.
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Email: paul.carpenter.epma-at-gmail.com Name: Paul Carpenter
Organization: MAS
Title-Subject: [Filtered] MAS Electron-Probe Microanalysis 2016 workshop
Message: Microanalysis Society Topical Conference Electron-Probe Microanalysis 2016
May 16-19, 2016 at the University of Wisconsin-Madison, WI
Registration is now open See website for full details Abstract deadline February 15, 2016 MAS Early Career Scholar financial travel support
Four-day intensive MAS topical conference
User meetings with EPMA 2016 sponsors on Monday, May 16, 2016 Three-day intensive topical conference, Tues-Thurs May 17-19, 2016 - EPMA, SEM, WDS, EDS techniques
Tutorial on EPMA and SEM quantitative analysis
Topics with invited and contributed presentations
General electron-probe microanalysis: analysis, instrumentation, methods
Trace element EPMA: background methods, high-resolution EPMA
Advanced WDS and EDS techniques, Monte Carlo simulation, accuracy studies
Quantitative compositional mapping by EPMA
Cathodoluminescence
Presentations and product information from the microanalysis vendor community
Inexpensive housing options
Group meals and discussion
Problems identified by the microanalysis community -- solutions provided by the vendor community
Contact Paul Carpenter for more information: paul.carpenter.epma-at-gmail.com
Paul Carpenter Earth and Planetary Sciences CB1169 Washington University 1 Brookings Drive Saint Louis, MO 63130 314-602-9697
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Email: mk-at-seclab.tuwien.ac.at Name: Markus Kammerstetter
Organization: Secure Systems Lab Vienna, Vienna University of Technology
Title-Subject: [Filtered] Looking for EDS for RJ Lee PSEM
Message: Hello,
in our lab we have an older model RJ Lee/Aspex PSEM Scanning Electron Microscope in use to image and analyze microchips. We deprocess those chips layer by layer but currently do not have the exact information of the material compositions used in those layers. Since the deprocessing and material removal techniques (i.e. wet etching) heavily depend on the materials, we have been looking for an EDS system for quite a while but unfortunately do not really have a budget for it.
It would thus be great if anyone on this list could spare a surplus EDS that is no longer needed (detector, control HW and possibly also the SW tool). Ideally it would include light elements and be compatible with the Aspex/RJ Lee PSEM so that we could use the PSEM's software. However, virtually any EDS would also be nice to have if we can make it fit. Also just heavy elements are fine as this would still allow us to characterize Si, Cu, Al, W, Au and other material compositions typically found in integrated circuits.
We have access to LN2 for cooling, we can typically do mechanical and electronics repairs on our own and we could also mill a custom adapter to make it fit onto our PSEM port.
Thank you, Markus
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Email: lnbrewer1-at-eng.ua.edu Name: Luke Brewer
Organization: University of Alabama
Title-Subject: [Filtered] Inviting you to attend and participate in EBSD 2016
Message: Dear Colleagues,
I invite you to attend and participate in Electron Backscatter Diffraction 2016! EBSD 2016 will be held at The University of Alabama from May 24-26, 2016.
EBSD 2016 will have a one-day tutorial session with both lectures and hands-on laboratory demonstrations designed to introduce EBSD to students and professionals new to the technique. There will also be more advanced tutorials for experienced users on topics such as precession electron diffraction, high angular resolution EBSD, and transmission kikuchi diffraction.
Days 2-3 will have platform presentations and posters devoted to the latest in EBSD-technique development and applications to materials science, geology and earth sciences, and materials engineering.
Support will be available to encourage students to attend this meeting.
Registration is now open, and we are accepting abstracts on-line until March 1, 2016.
Full details on the meeting can be found at: http://www.microbeamanalysis.org/topical-conferences/ebsd-2016/welcome
Please let me know if you have any questions, and we do hope to see you at EBSD 2016!
Luke Brewer
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I am having problems finding pre-cleaned microscope slides that are actually clean. I've looked at recently purchased slides form 2 vendors but neither product is "clean" with easily visible particulates and splotchy thin films randomly covering slide surfaces. Is anyone else out there noticing that previously good sources are now "dirty". I am using Polarized and bright field illumination. I would appreciate a product recommendation if you currently have a high quality source.
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Organization: OSU Center for Veterinary Health Sciences
Title-Subject: [Filtered] Seeking a repairperson for a Sorvall MT7000
Message: If anyone has the name and contact information for a person who could provide service for a Sorvall MT-7000, please share this information with me.
Thanks very much.
Kathy Kocan
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Email: wzhe-at-laurentian.ca Name: William
Organization: Laurentian University
Title-Subject: [Filtered] EDS detector window problem
Message: Hello,
We currently had problem with EDS SDD detector , it couldn-it be cooling down and a -eFault-i message for detector condition came out at the end. One recommendation is that is sign of detector window blown or pin hole on it. Is somebody ever went through this type of issue and have ideas about
1. how to identify if it is a window problem? 2. is it a simple job to replace a defective window if you have parts, like people always do for WDS detector on their own ? If not, 3. any service company offers repairing in north America you may recommend ?
Thanks so much in advance,
William
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Application Deadline Monday, January 25. Please spread the word.
Dear Colleagues,
We are excited to announce this year's run of Analytical and Quantitative Light Microscopy, held at the Marine Biological Laboratory, Woods Hole, MA, USA. The course will run May 4 - May 13 , 2016.
AQLM is a comprehensive and intensive course in light microscopy for researchers in biology, medicine, and material sciences. This course provides a systematic and in-depth examination of the theory of image formation and application of digital methods for exploring subtle interactions between light and the specimen. This course emphasizes the quantitative issues that are critical to the proper interpretation of images obtained with modern wide-field and confocal microscopes.
Laboratory exercises, demonstrations, and discussions include: * geometrical and physical optics of microscope image formation including Abbe's theory of the microscope and Fourier optics; * phase contrast, polarization and interference microscopy; * fluorescence microscopy, quantification of fluorescence, and fluorescent proteins; * principles and application of digital imaging and quantitative digital image deconvolution; * digital image processing and object identification and tracking; * live cell and ratiometric imaging for FRET and ion concentration imaging; * confocal microscopy and specialized methods such as TIRF and FLIM; and * new advances in light microscopy such as FCS, PALM, STORM, SIM and SPIM.
The course web site is at http://www.mbl.edu/aqlm .
Within the course, we often refer to AQLM as "Microscopy Boot Camp". We cover every topic with a lecture and an in-depth hands-on lab. It's intense, it's hardcore, and it's really fun.
Why not join us for AQLM (or recommend one of your lab members or core facility users)? You'll never forget it.
Applications due January 25 , 2016.
Directors: Jagesh Shah (Harvard Medical School), Justin Taraska (National Institutes of Health) Course Laboratory Director: Wendy Salmon (Whitehead Institute/MIT)
Cheers, Jagesh, Justin and Wendy
Posters available at http://www.mbl.edu/education/files/2014/04/AQLM_POSTER_2016v1.pdf
~~~~~~~~~~~~~~~~~~~~~~~ Wendy Salmon Light Microscopy Specialist Whitehead Institute for Biomedical Research W.M. Keck Imaging Facility 9 Cambridge Center, Rm 447 Cambridge, MA 02142 c: 617-429-0158 e: wsalmon-at-wi.mit.edu w: http://staffa.wi.mit.edu/microscopy/
==============================Original Headers============================== 13, 40 -- From wsalmon-at-wi.mit.edu Sat Jan 23 12:33:24 2016 13, 40 -- Received: from ptolemy.wi.mit.edu (ptolemy.wi.mit.edu [18.4.1.120]) 13, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0NIXIGM016281 13, 40 -- for {microscopy-at-microscopy.com} ; Sat, 23 Jan 2016 12:33:19 -0600 13, 40 -- X-IronPort-AV: E=Sophos;i="5.22,333,1449550800"; 13, 40 -- d="scan'208";a="3640678" 13, 40 -- Received: from unknown (HELO mars.wi.mit.edu) ([10.9.4.20]) 13, 40 -- by ptolemy.wi.mit.edu with ESMTP; 22 Jan 2016 16:11:38 -0500 13, 40 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 13, 40 -- by mars.wi.mit.edu (Postfix) with ESMTP id B7D2631E138 13, 40 -- for {microscopy-at-microscopy.com} ; Fri, 22 Jan 2016 16:11:38 -0500 (EST) 13, 40 -- Received: from mars.wi.mit.edu ([127.0.0.1]) 13, 40 -- by localhost (mars.wi.mit.edu [127.0.0.1]) (amavisd-new, port 10032) 13, 40 -- with ESMTP id TZ2FtvMjteGg for {microscopy-at-microscopy.com} ; 13, 40 -- Fri, 22 Jan 2016 16:11:36 -0500 (EST) 13, 40 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 13, 40 -- by mars.wi.mit.edu (Postfix) with ESMTP id 931EE31E13E 13, 40 -- for {microscopy-at-microscopy.com} ; Fri, 22 Jan 2016 16:11:36 -0500 (EST) 13, 40 -- X-Virus-Scanned: amavisd-new at mars.wi.mit.edu 13, 40 -- Received: from mars.wi.mit.edu ([127.0.0.1]) 13, 40 -- by localhost (mars.wi.mit.edu [127.0.0.1]) (amavisd-new, port 10026) 13, 40 -- with ESMTP id MJ_iGgBykbZT for {microscopy-at-microscopy.com} ; 13, 40 -- Fri, 22 Jan 2016 16:11:36 -0500 (EST) 13, 40 -- Received: from io.wi.mit.edu (io.wi.mit.edu [10.9.4.21]) 13, 40 -- by mars.wi.mit.edu (Postfix) with ESMTP id 77FD131E138 13, 40 -- for {microscopy-at-microscopy.com} ; Fri, 22 Jan 2016 16:11:36 -0500 (EST) 13, 40 -- Date: Fri, 22 Jan 2016 16:11:36 -0500 (EST) 13, 40 -- From: Wendy Salmon {wsalmon-at-wi.mit.edu} 13, 40 -- To: microscopy {microscopy-at-microscopy.com} 13, 40 -- Message-ID: {344470042.11777.1453497096429.JavaMail.zimbra-at-wi.mit.edu} 13, 40 -- In-Reply-To: {816105877.10462683.1452116518458.JavaMail.zimbra-at-wi.mit.edu} 13, 40 -- References: {816105877.10462683.1452116518458.JavaMail.zimbra-at-wi.mit.edu} 13, 40 -- Subject: LM - 2016 Analytical and Quantitative Light Microscopy Course -at- MBL 13, 40 -- MIME-Version: 1.0 13, 40 -- Content-Type: text/plain; charset=utf-8 13, 40 -- Content-Transfer-Encoding: 7bit 13, 40 -- X-Originating-IP: [134.174.140.59] 13, 40 -- X-Mailer: Zimbra 8.6.0_GA_1191 (ZimbraWebClient - GC47 (Mac)/8.6.0_GA_1191) 13, 40 -- Thread-Topic: LM - 2016 Analytical and Quantitative Light Microscopy Course -at- MBL 13, 40 -- Thread-Index: 3I4RFXAc6NuHKgHiTFr8kP+ljEXmnAHyDNBp ==============================End of - Headers==============================
I would like to draw your attention to the call for nominations for the Gjonnes Medal at http://www.iucr.org/resources/commissions/electron-crystallography/gjonnes-medal
The Gj/PInnes Medal in Electron Crystallography is awarded every three years and recognizes an outstanding contribution to the field of electron crystallography. Nomination for the Gj/PInnes Medal is open to scientists and engineers in all areas of electron crystallography defined in the broadest context as the branch of science that uses electron scattering and imaging to study the structure of matter.
Please forward as appropriate.
-- Professor Laurence Marks Department of Materials Science and Engineering Northwestern University www.numis.northwestern.edu Corrosion in 4D: MURI4D.numis.northwestern.edu Co-Editor, Acta Cryst A "Research is to see what everybody else has seen, and to think what nobody else has thought" Albert Szent-Gyorgi
==============================Original Headers============================== 6, 41 -- From marksmsa-at-gmail.com Mon Jan 25 07:56:48 2016 6, 41 -- Received: from mail-lb0-f169.google.com (mail-lb0-f169.google.com [209.85.217.169]) 6, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0PDul4Z011783 6, 41 -- for {Microscopy-at-microscopy.com} ; Mon, 25 Jan 2016 07:56:48 -0600 6, 41 -- Received: by mail-lb0-f169.google.com with SMTP id oh2so74306465lbb.3 6, 41 -- for {Microscopy-at-microscopy.com} ; Mon, 25 Jan 2016 05:56:36 -0800 (PST) 6, 41 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 41 -- d=gmail.com; s=20120113; 6, 41 -- h=mime-version:date:message-id:subject:from:to:content-type 6, 41 -- :content-transfer-encoding; 6, 41 -- bh=eJTRCxW18amnX96ZxQah12BYXFKTQa7Ha1jEYoMZ138=; 6, 41 -- b=gyVIcug9pncp9WWAZoo5ERd9HCF60vuBClLojdqissl/LR3abuvNmGRVjv8Wcaxi7A 6, 41 -- 76pH4mAU/g90jRjjvrzNsjzY/leEx2i3J0F+X6eVtOB8m8KB+C6dYiPAM6tiXsZ8QnjC 6, 41 -- r1osfEtmJQCnQwvA9YcUTaLO5LObo+YfY1Sl5LcVz4PNTNtgZuyk0WbIHeJbfMjFkswH 6, 41 -- LxzO3k1mRiCTJS/iUpo4tEjMuiVgg8FtQr9IK0UhN02DMi3mMZlE5dCQ3PoKOu20hRu+ 6, 41 -- 6uzh3R6QyOFSQ/T7C9b0dtm13kmegdsqGkuhYJLmM2xqcFO+avrEdP1LgbxrkiRu7boI 6, 41 -- Jfuw== 6, 41 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 41 -- d=1e100.net; s=20130820; 6, 41 -- h=x-gm-message-state:mime-version:date:message-id:subject:from:to 6, 41 -- :content-type:content-transfer-encoding; 6, 41 -- bh=eJTRCxW18amnX96ZxQah12BYXFKTQa7Ha1jEYoMZ138=; 6, 41 -- b=Ny+1nrnvTJkt0t8NPQ2a7jTY8NAD1+5S9RbTT5RsNaGw6DPJIvbr2xo/58TVCiUlqW 6, 41 -- waI+VhDdyTu9jQRSZKm/ialtDWCddEW3TCg04OnkR0+mMeCHc3pXUvPlJRl0rYYiyllU 6, 41 -- IKQa/4FMJeJWJG0MWa3RLweZ/e3cyfgsaZ8Um33HTJROA3050DWq/2Al6itos3yc/9+U 6, 41 -- 6Kvgk6KOScryunGvpvBKI8DZqcGD/ZcCwUFKcNDPWhisEWME6+Rmr7cMsLqgzO9NkH/f 6, 41 -- 6kelqa9XxpaRRW+TtfOSS53cg0r5QTQ/beW+kvwm4ajTIhimGn/F2unmsBpDoQvFFa8z 6, 41 -- QhiA== 6, 41 -- X-Gm-Message-State: AG10YORXrJCnmVDOH8bqtrsumFzWaXn8w4NLkuUSDZUgHLcuxmnHmtHhmfMVDtZnivIvUy9DL8JiVTKL6TOHxA== 6, 41 -- MIME-Version: 1.0 6, 41 -- X-Received: by 10.112.73.41 with SMTP id i9mr1453083lbv.128.1453475883194; 6, 41 -- Fri, 22 Jan 2016 07:18:03 -0800 (PST) 6, 41 -- Received: by 10.112.126.228 with HTTP; Fri, 22 Jan 2016 07:18:03 -0800 (PST) 6, 41 -- Date: Fri, 22 Jan 2016 09:18:03 -0600 6, 41 -- Message-ID: {CAHuu12pZ6bd3S_XxFKtc6sBCFB3bT439A0zhhED7K+PV5cX7Fg-at-mail.gmail.com} 6, 41 -- Subject: 6, 41 -- From: L Marks {marksmsa-at-gmail.com} 6, 41 -- To: microscopy {Microscopy-at-microscopy.com} 6, 41 -- Content-Type: text/plain; charset=UTF-8 6, 41 -- Content-Transfer-Encoding: 8bit 6, 41 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u0PDul4Z011783 ==============================End of - Headers==============================
I wrote a software called stoichometry fitter for doing quick analyses of spectra acquired by EDS (or XRF). Originally, this was just for my own use but it has evolved to the point where I think it may be useful for some of you. I have now made it open source and put it on GitHub for anyone who wants to use it or contribute to it.
As input it takes At%, Wt% or OxWt% values.
It has the ability to fit several phases, for example, if you have a pyroxene which you think is contaminated with a bit of FeS, you can fit against a pyroxene triangle + FeS, and it will give you the correct EnFsWo values after removing the appropriate amount of Fe for the FeS. It also has a freeform analysis capability for a few minerals I use, and I'm hoping others will contribute their own analyses as well so we can all use them. For example, if you tell it the spectrum is a pyroxene, then it will compute T, M1 and M2 sites for all the elements using the Morimoto 1988 reference. If you are handy with python and numpy then you can create your own analyses with a minimum of four lines of code.
Finally, it has a TEM k-factor + thickness correction capability if you input each element as counts instead of the usual At%/Wt%/OxWt%. This is very useful if you have a TEM, but it would also apply to some thin-film experiments in an SEM/EPMA.
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Email: jhyun-at-gatan.com Name: John Hyun
Organization: Gatan, Inc.
Title-Subject: [Filtered] EELS & EFTEM Analysis Training School
Message: April 12-15, 2016 Gatan R&D Headquarters Pleasanton, CA, USA
This comprehensive hands-on training course reviews the basic theory and practice of EELS imaging and analysis in the TEM, but its main emphasis is on practical techniques, optimum deployment of EELS and EFTEM hardware and software systems, and advanced applications. Some prior experience with EELS, EFTEM, and energy filter systems is recommended, as is a good familiarity with TEM/STEM instrumentation and techniques. By the end of the course, participants can expect to know how best to optimize the performance of their EELS hardware as well as their EELS and EFTEM experimental setups in order to capture and extract the maximum amount of information from their TEM samples. Most of the curriculum is devoted to live microscope and computer lab sessions.
Topics:
-iFundamentals of EELS and energy-filtered imaging in TEM -iPrinciples of operation of EFTEM and EELS systems -iOptimization of EFTEM and EELS data acquisition -iQuantification of elemental composition -iOther information provided by EFTEM/EELS and how best to extract it -iUse of EELS signals to form maps of elemental and chemical composition -iEFTEM and STEM EELS spectrum imaging techniques -iIdentification of material phases via EELS fine structure mapping -iApplications to biological and physical science specimens
Complete information and online registration is available at: http://www.gatan.com/company/events/eels-eftem-analysis-training-school-april-2016
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Email: david-at-electronmicro.com Name: David Scharf
Organization: SCHARF MICROSCOPY
Title-Subject: [Filtered] LEO440 CPU CMOS battery
Message: Can anyone help? I am getting a message that my CMOS battery has failed upon booting up my LEO440 SEM. I have looked everywhere I can think of and cannot find that battery to replace it. It is NOT on the computer board for sure! Must be external somewhere. Anyone service this instrument before and know where its located? No mention of its location in my service manuals either. Thanks in advance for any help.
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Title-Subject: [Filtered] TEM - Cryo-ultramicrotomy of rubber sections
Message: Can anyone recommend a technique for collecting rubber sections during cryo-ultramicrotomy that enables the sections to lie flat (i.e. as wrinkle free as possible) on a TEM support grid? I currently collect my sections off the back of my cryoknife using a sucrose droplet, but as the sections warm coming out of the cryochamber on the droplet they contract and wrinkle before being deposited on a TEM grid (with or without carbon coating). Chamber temperature is -120 C. Any ideas are appreciated. Thank you.
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Symposium in Advanced Electron Microscopy in Nanostructure Research and Inauguration of the new Titan Themis 300 S/TEM.
We are glad to announce and invite you to a full-day symposium in iAdvanced Electron Microscopy in Nanostructure Researchi and the inauguration of the new Titan Themis 300 S/TEM and the Quanta 200 duo beam instrument at the Central Facility for Advanced Microscopy and Microanalysis (CFAMM).
The inauguration will take place on March 10th, 2016 starting at 9 a.m. in 355 HUB building at the University of California at Riverside. Plenary seminar talks will be presented by leading researchers demonstrating the application of S/TEM and duo-beam techniques in nanostructure research. The plenary session will be followed by demo sessions on the instruments in the afternoon.
Central Facility for Advanced Microscopy and Microanalysis, Research & Economic Development Office Materials Science and Engineering - BCOE University of California Riverside, CA 92521
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Email: wtkiii-at-hotmail.com Name: WILLIAM KING
Title-Subject: [Filtered] TEM & SEM of intestinal cells
Message: I would like to know why the antigen behind Ulcerative Colitis has not been identified. If the affected tissue were available, how many micrographs would it take to provide some visual cues?
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Email: nxa157-at-case.edu Name: Nanthawan Avishai
Organization: Microscopy Society of Northeastern Ohio, MSNO
Message: Our MSNO Winter Meeting 2016 will go to a new and exciting place: Lorain County Community College. The meeting will be on March 2nd, 2016 3-7:15 pm. Please see the full program from attached below.
The event will combine professional networking, lectures on by leading expert, guided tours & demos at the impressive LCCC Desich Center, student posters, exhibitions by leading vendors in the field, a nice dinner, a fun raffle and much more. Here are some of the highlights: - Two main talks on Applications for MEMS Sensors (Matthew Apanius, Managing Director, SMART Microsystems, Ltd) and "Basic Digital Imaging and Image Form" (by Dr. W. Gray Jerome, Vanderbilt University Medical Center, a National MSA tour speaker).
- Tour at the Desich Center
The registration and more detail of this meeting can be found at http://www.msneo.org/2016-winter-meeting.html
Looking forward to seeing you at LCCC! MSNO Board
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One or two of my users have observed a strange behavior on our FEI Talos F200X (FEI SuperX detector, Bruker pulse processor, Bruker software).
I'm not going to call this an "error" or a "fault," just something strange. Hopefully the list's hive mind can reality check me.
Let's say that on a very thin, low-Z sample, 400 pA might give 400 counts / sec on each of the four detectors (as seen in the TIA SuperX tab). 300 pA might give 300 cps. 200 pA might give zero. (Numbers are approximate.)
I interpret this to mean that there is a low-end discriminator on the pulse processor or software somewhere, and below a certain count rate no counts are registering. Is this possible? Is there a setting I can drill down to in order to let my users push to lower beam currents on their beam-sensitive samples?
This isn't a common problem, most of my Talos users are running ~5 nA, ~200 kcps beautifully, but I want to help all my users, not just those who can use the STEM as an electron sledgehammer.
Thanks in advance Chad Parish
--------------------- Chad M. Parish, Ph.D. Research and Development Staff Member Radiation Effects and Microstructural Analysis Group Materials Science and Technology Division Oak Ridge National Laboratory Phone: 1 865 574 0092 Email: parishcm-at-ornl.gov
==============================Original Headers============================== 10, 53 -- From parishcm-at-ornl.gov Thu Jan 28 06:52:26 2016 10, 53 -- Received: from mta02.ornl.gov (mta02.ornl.gov [128.219.177.12]) 10, 53 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0SCqQkd021923 10, 53 -- for {Microscopy-at-microscopy.com} ; Thu, 28 Jan 2016 06:52:26 -0600 10, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 10, 53 -- d=ornl.gov; i=-at-ornl.gov; q=dns/txt; s=p20151116; 10, 53 -- t=1453985534; x=1485521534; 10, 53 -- h=from:to:subject:date:message-id: 10, 53 -- content-transfer-encoding:mime-version; 10, 53 -- bh=0K5YMDyPwYQooheRgxj/FTgKf0AHz08TVaqOkaSqnjk=; 10, 53 -- b=JQHfmgS1J7qCem6b4i9bUlY4nbNpNCehgsPzwhe5Ww+0fgUAvLHIzX2F 10, 53 -- HXJaUjP8fWBzrbD13bzEFwAE9DsHX320NirvzY78txNEbfxvWswwFMLz9 10, 53 -- j87523vFotYQsRFDnKxi2afbOHAkx5g4UkuIpYOXjuOKQG+SzzAdhjq+b 10, 53 -- 3pWaGGmnm9pGLVUkl893mvPox9XMfryEjuwEqrxo2b9KqKsXreT1HzNco 10, 53 -- yHl3l0oB3DLTLWU5JfeR4RcV4LyJs8g8YmQ2JWeSLl4ugPSrnbAkJwzgz 10, 53 -- AI/6xFhSZnwnR3fLyJx/Udz8vTLqC6DAa9wK8ncJSmFRD+kTEuMO90/n7 10, 53 -- w==; 10, 53 -- X-SG: RELAYLIST 10, 53 -- X-IronPort-AV: E=Sophos;i="5.22,358,1449550800"; 10, 53 -- d="scan'208";a="96755483" 10, 53 -- Received: from emgwy1.ornl.gov ([160.91.254.9]) 10, 53 -- by iron2.ornl.gov with ESMTP/TLS/DHE-RSA-AES256-GCM-SHA384; 28 Jan 2016 07:52:13 -0500 10, 53 -- Received: from EXCHOS31.ornl.gov (exchos31.ornl.gov [128.219.12.151]) 10, 53 -- (using TLSv1 with cipher ECDHE-RSA-AES256-SHA (256/256 bits)) 10, 53 -- (No client certificate requested) 10, 53 -- by emgwy1.ornl.gov (Postfix) with ESMTPS id 3prhXF6W9Zz7tK9 10, 53 -- for {Microscopy-at-Microscopy.Com} ; Thu, 28 Jan 2016 07:52:13 -0500 (EST) 10, 53 -- Received: from EXCHCS34.ornl.gov (128.219.12.148) by EXCHOS31.ornl.gov 10, 53 -- (128.219.12.151) with Microsoft SMTP Server (TLS) id 15.0.1104.5; Thu, 28 Jan 10, 53 -- 2016 07:52:13 -0500 10, 53 -- Received: from EXCHCS32.ornl.gov (128.219.12.146) by EXCHCS34.ornl.gov 10, 53 -- (128.219.12.148) with Microsoft SMTP Server (TLS) id 15.0.1104.5; Thu, 28 Jan 10, 53 -- 2016 07:52:13 -0500 10, 53 -- Received: from EXCHCS32.ornl.gov ([fe80::2408:8b8c:e63d:2a33]) by 10, 53 -- EXCHCS32.ornl.gov ([fe80::2408:8b8c:e63d:2a33%17]) with mapi id 10, 53 -- 15.00.1104.000; Thu, 28 Jan 2016 07:52:13 -0500 10, 53 -- From: "Parish, Chad M." {parishcm-at-ornl.gov} 10, 53 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-microscopy.com} 10, 53 -- Subject: FEI SuperX / Bruker: Strange performance at very low count rates 10, 53 -- Thread-Topic: FEI SuperX / Bruker: Strange performance at very low count rates 10, 53 -- Thread-Index: AdFZyrKEswZ9BvjyTQmAzHMpryukEA== 10, 53 -- Date: Thu, 28 Jan 2016 12:52:12 +0000 10, 53 -- Message-ID: {16c8fb3650e14a9981accfc0506e11b5-at-EXCHCS32.ornl.gov} 10, 53 -- Accept-Language: en-US 10, 53 -- Content-Language: en-US 10, 53 -- X-MS-Has-Attach: 10, 53 -- X-MS-TNEF-Correlator: 10, 53 -- x-ms-exchange-transport-fromentityheader: Hosted 10, 53 -- x-originating-ip: [128.219.12.132] 10, 53 -- Content-Type: text/plain; charset="us-ascii" 10, 53 -- MIME-Version: 1.0 10, 53 -- Content-Transfer-Encoding: 8bit 10, 53 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u0SCqQkd021923 ==============================End of - Headers==============================
The Hooke College of Applied Sciences, located in Westmont, IL, is offering its scanning electron microscopy short course March 14-18, 2016.*
In addition to lectures, this course emphasizes hands-on training using state-of-the-art equipment.* For further SEM training details and registration information, please follow the link below:
Best regards- __________________________________________________ Chris Gorman Admissions Specialist Hooke College of Applied Sciences 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7412(fax) www.hookecollege.com
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we have a JEOL X-ray detector with a Thermo Pulse-processor and software and we can go way down in beam current to still collect X-rays. On a recent trip our engineer showed me a software switch to remove the "noise" peak counts from the spectrum and the reported count rate.
But if you are literally not seeing any peaks with 200pA then there's a fundamental problem - call Bruker at once.
Good luck.
Rob Keyse
On 1/28/2016 8:12 AM, parishcm-at-ornl.gov wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Listers, } } One or two of my users have observed a strange behavior on our FEI Talos F200X (FEI SuperX detector, Bruker pulse processor, Bruker software). } } I'm not going to call this an "error" or a "fault," just something strange. Hopefully the list's hive mind can reality check me. } } Let's say that on a very thin, low-Z sample, 400 pA might give 400 counts / sec on each of the four detectors (as seen in the TIA SuperX tab). 300 pA might give 300 cps. 200 pA might give zero. (Numbers are approximate.) } } I interpret this to mean that there is a low-end discriminator on the pulse processor or software somewhere, and below a certain count rate no counts are registering. Is this possible? Is there a setting I can drill down to in order to let my users push to lower beam currents on their beam-sensitive samples? } } This isn't a common problem, most of my Talos users are running ~5 nA, ~200 kcps beautifully, but I want to help all my users, not just those who can use the STEM as an electron sledgehammer. } } Thanks in advance } Chad Parish } } --------------------- } Chad M. Parish, Ph.D. } Research and Development Staff Member } Radiation Effects and Microstructural Analysis Group } Materials Science and Technology Division } Oak Ridge National Laboratory } Phone: 1 865 574 0092 } Email: parishcm-at-ornl.gov } } } } ==============================Original Headers============================== } 10, 53 -- From parishcm-at-ornl.gov Thu Jan 28 06:52:26 2016 } 10, 53 -- Received: from mta02.ornl.gov (mta02.ornl.gov [128.219.177.12]) } 10, 53 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0SCqQkd021923 } 10, 53 -- for {Microscopy-at-microscopy.com} ; Thu, 28 Jan 2016 06:52:26 -0600 } 10, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 10, 53 -- d=ornl.gov; i=-at-ornl.gov; q=dns/txt; s=p20151116; } 10, 53 -- t=1453985534; x=1485521534; } 10, 53 -- h=from:to:subject:date:message-id: } 10, 53 -- content-transfer-encoding:mime-version; } 10, 53 -- bh=0K5YMDyPwYQooheRgxj/FTgKf0AHz08TVaqOkaSqnjk=; } 10, 53 -- b=JQHfmgS1J7qCem6b4i9bUlY4nbNpNCehgsPzwhe5Ww+0fgUAvLHIzX2F } 10, 53 -- HXJaUjP8fWBzrbD13bzEFwAE9DsHX320NirvzY78txNEbfxvWswwFMLz9 } 10, 53 -- j87523vFotYQsRFDnKxi2afbOHAkx5g4UkuIpYOXjuOKQG+SzzAdhjq+b } 10, 53 -- 3pWaGGmnm9pGLVUkl893mvPox9XMfryEjuwEqrxo2b9KqKsXreT1HzNco } 10, 53 -- yHl3l0oB3DLTLWU5JfeR4RcV4LyJs8g8YmQ2JWeSLl4ugPSrnbAkJwzgz } 10, 53 -- AI/6xFhSZnwnR3fLyJx/Udz8vTLqC6DAa9wK8ncJSmFRD+kTEuMO90/n7 } 10, 53 -- w==; } 10, 53 -- X-SG: RELAYLIST } 10, 53 -- X-IronPort-AV: E=Sophos;i="5.22,358,1449550800"; } 10, 53 -- d="scan'208";a="96755483" } 10, 53 -- Received: from emgwy1.ornl.gov ([160.91.254.9]) } 10, 53 -- by iron2.ornl.gov with ESMTP/TLS/DHE-RSA-AES256-GCM-SHA384; 28 Jan 2016 07:52:13 -0500 } 10, 53 -- Received: from EXCHOS31.ornl.gov (exchos31.ornl.gov [128.219.12.151]) } 10, 53 -- (using TLSv1 with cipher ECDHE-RSA-AES256-SHA (256/256 bits)) } 10, 53 -- (No client certificate requested) } 10, 53 -- by emgwy1.ornl.gov (Postfix) with ESMTPS id 3prhXF6W9Zz7tK9 } 10, 53 -- for {Microscopy-at-Microscopy.Com} ; Thu, 28 Jan 2016 07:52:13 -0500 (EST) } 10, 53 -- Received: from EXCHCS34.ornl.gov (128.219.12.148) by EXCHOS31.ornl.gov } 10, 53 -- (128.219.12.151) with Microsoft SMTP Server (TLS) id 15.0.1104.5; Thu, 28 Jan } 10, 53 -- 2016 07:52:13 -0500 } 10, 53 -- Received: from EXCHCS32.ornl.gov (128.219.12.146) by EXCHCS34.ornl.gov } 10, 53 -- (128.219.12.148) with Microsoft SMTP Server (TLS) id 15.0.1104.5; Thu, 28 Jan } 10, 53 -- 2016 07:52:13 -0500 } 10, 53 -- Received: from EXCHCS32.ornl.gov ([fe80::2408:8b8c:e63d:2a33]) by } 10, 53 -- EXCHCS32.ornl.gov ([fe80::2408:8b8c:e63d:2a33%17]) with mapi id } 10, 53 -- 15.00.1104.000; Thu, 28 Jan 2016 07:52:13 -0500 } 10, 53 -- From: "Parish, Chad M." {parishcm-at-ornl.gov} } 10, 53 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-microscopy.com} } 10, 53 -- Subject: FEI SuperX / Bruker: Strange performance at very low count rates } 10, 53 -- Thread-Topic: FEI SuperX / Bruker: Strange performance at very low count rates } 10, 53 -- Thread-Index: AdFZyrKEswZ9BvjyTQmAzHMpryukEA== } 10, 53 -- Date: Thu, 28 Jan 2016 12:52:12 +0000 } 10, 53 -- Message-ID: {16c8fb3650e14a9981accfc0506e11b5-at-EXCHCS32.ornl.gov} } 10, 53 -- Accept-Language: en-US } 10, 53 -- Content-Language: en-US } 10, 53 -- X-MS-Has-Attach: } 10, 53 -- X-MS-TNEF-Correlator: } 10, 53 -- x-ms-exchange-transport-fromentityheader: Hosted } 10, 53 -- x-originating-ip: [128.219.12.132] } 10, 53 -- Content-Type: text/plain; charset="us-ascii" } 10, 53 -- MIME-Version: 1.0 } 10, 53 -- Content-Transfer-Encoding: 8bit } 10, 53 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u0SCqQkd021923 } ==============================End of - Headers==============================
-- Robert Keyse, DPhil Research Scientist Lehigh University
5, East Packer Avenue, Whitaker Laboratory Bethlehem, PA 18015-3194
Office (610)758-3465 Fax (610)758-4244
==============================Original Headers============================== 10, 33 -- From rok210-at-lehigh.edu Thu Jan 28 08:22:19 2016 10, 33 -- Received: from rain4.cc.lehigh.edu (rain4.cc.lehigh.edu [128.180.3.218]) 10, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0SEMJmr000752 10, 33 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jan 2016 08:22:19 -0600 10, 33 -- Received: from [127.0.0.1] (rok210pc.mat.lehigh.edu [128.180.54.183]) 10, 33 -- (Authenticated sender: rok210) 10, 33 -- by rain4.cc.lehigh.edu (Postfix) with ESMTPSA id 0CEAA503AF87; 10, 33 -- Thu, 28 Jan 2016 09:22:07 -0500 (EST) 10, 33 -- Subject: Re: [Microscopy] FEI SuperX / Bruker: Strange performance at very low 10, 33 -- count rates 10, 33 -- To: parishcm-at-ornl.gov, microscopy-at-microscopy.com 10, 33 -- References: {201601281312.u0SDCisw001826-at-ns.microscopy.com} 10, 33 -- From: Robert Keyse {rok210-at-lehigh.edu} 10, 33 -- Message-ID: {56AA240E.7040000-at-lehigh.edu} 10, 33 -- Date: Thu, 28 Jan 2016 09:22:06 -0500 10, 33 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:38.0) Gecko/20100101 10, 33 -- Thunderbird/38.5.1 10, 33 -- MIME-Version: 1.0 10, 33 -- In-Reply-To: {201601281312.u0SDCisw001826-at-ns.microscopy.com} 10, 33 -- Content-Type: text/plain; charset=windows-1252; format=flowed 10, 33 -- Content-Transfer-Encoding: 7bit 10, 33 -- X-Antivirus: avast! (VPS 160128-0, 01/28/2016), Outbound message 10, 33 -- X-Antivirus-Status: Clean 10, 33 -- X-Virus-Scanned: clamav-milter 0.98.7 at rain4.CC.lehigh.EDU 10, 33 -- X-Virus-Status: Clean 10, 33 -- X-Spam-Status: No, score=-4.0 required=5.0 tests=ALL_TRUSTED,LU_FORGED_SENDER, 10, 33 -- RP_MATCHES_RCVD autolearn=disabled version=3.4.1 10, 33 -- X-Spam-Report: 10, 33 -- * -5.0 ALL_TRUSTED Passed through trusted hosts only via SMTP 10, 33 -- * -0.0 RP_MATCHES_RCVD Envelope sender domain matches handover relay domain 10, 33 -- * 1.0 LU_FORGED_SENDER No description available. 10, 33 -- X-Spam-Checker-Version: SpamAssassin 3.4.1 (2015-04-28) on 10, 33 -- freddie.cc.lehigh.edu ==============================End of - Headers==============================
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Email: ming_j_zhang-at-amat.com Name: Ming Zhang
Organization: Applied Materials Company
Title-Subject: [Filtered] FIB Technician Opening
Message: The successful candidate will focus on TEM sample preparations by hands-on operations of ex-situ and in-situ lift-out systems.
Term: Must be available for a minimum of 6 months. Term could be extended to 18 months. Highly possible to turn into a regular full time position in 6-12 months.
Work Schedule: Weekend and night shifts.
Education: Associate degree or equivalent combination of education and experience in Engineering, Materials Science, Chemistry, or Physics, etc.
Experience: Hands-on experience on FIBs or dual beams for TEM sample preparations. Manual TEM sample prep a plus.
Other Skills: Must be a team player and interact in a positive fashion with peers and customers. Must be able to work in a fast paced, multi-tasking environment where priorities may change often.
Location: Portland, Oregon.
US citizen or green card holder preferred.
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Long ago, when histology was in vogue, I had a problem during the summer producing paraffin sections of good repute when the windows were full open, the temp was above 80, humidity was high and Summer was the bane of my progress.
If you have a fancy microtome that has a means of changing the angle of the knife, then you can refine the method that I used with a self-constructed series of thin, plywood templates that would permit me to change the setting of my blade as the weather moved thru its cycle. My goal, when the change was in the wind, was to produce a section whose height was greater than all the others I could produce with angles above and below the one that worked the best.
Air conditioning had just arrived, but not in our lab, so, I was stuck with my templates. Since I was working in a building with large windows, no cooling but the breeze on the mountain, patience and changing angles and, oh yes, changing the hardness of the paraffin as the weather went thru its yearly cycle. If one moved South to Florida to cut sections, one would have to fabricate a Florida paraffin mixture as an embedment - much too hard for even Spring or Fall in Bethlehem, PA.
So, we come to the advice. The first problem is compression. The second is the sharpness of the blade. The third is the vibrations that emanate from the microtome that can, in the first approximation, be reduced with a few drops of lubricant. Then, there is the vibration of the floor, the building, and the planet, and the solidity of the blade in its holder, the geometry of the blade and the angles of the cutting wedge on the edge of the blade. Sorry, I digress.
The optimal angle lies within a small range of not-so-optimal angles that are dependent on the hardness of the paraffin or plastic, the temp of the environment, the geometry of the cutting edge, the humidity, and everything else.
As I recall, my instructor, who had a PhD from Harvard under Alfred Romer, instructed me on the microtome and its personalities by advising me to, "fiddle with the block, the knife, the angle of the knife, the rate of the cutting cycle, and everything else until you get good sections." He ended with, "That's how I always did it."
Now that I've said and reviewed all of this I conclude that I have not been very helpful. That is primarily due to the fact that I haven't cut a section from a paraffin block since 1992, when I taught my younger daughter to cut sections for my research. As I recall, I used the word "fiddle" more than once during the tutorial, but again that was then and we sharpened our knives ourselves. She, by the way, was the best, and happiest 'cranker' I ever trained, and I convinced a small army to be good at it.
I hope that at the very least you are happy that you are younger, and don't have to do your sectioning in a room with the windows open - even in Winter!
Seicerely,
Fred Monson (he works on electron microscopes, hardly ever has to cut sections. and is contentedly moving toward 77 with good health and a lively sense of humor.
P.S. The adjustment of the knife angle is similar to the problem a baseball player has with his bat. He fiddles with everything to reduce the frequency of his pop ups. Neither are like the violin on which you are guaranteed that you will be world famous if you practice for 10,000 hours - either for the best or the worst noises you can project from the instrument.
Frederick C Monson, PhD Center for Microanalysis and Imaging, Research and Training (CMIRT) West Chester University of Pannsylvania West Chester, PA, 1938 610-738-0437 fmonson-at-wcupa.edu ________________________________________ X-from: Kelli Goodkowsky via Histonet {histonet-at-lists.utsouthwestern.edu} Sent: Friday, January 29, 2016 3:15 PM To: histonet-at-lists.utsouthwestern.edu
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Email: sbarlow-at-mail.sdsu.edu Name: Steve Barlow
Organization: SDSU EM Facility
Title-Subject: [Filtered] TEM: agar embedding cells
Message: Some advice please on colorizing agar for embedding cells. In the past, my osmicated pellets in agar blocks were easily trackable, but when the cells are in a thin layer the cells don't give much contrast, especially if not osmicated. I am collecting naked eukaryotic cells in suspension culture, but have problems bring down the smaller cells by centrifugation. Cultures containing cells are fixed in aldehyde or aldehyde/osmium cocktail, collected onto millipore Nucleopore filters, then held in place by adding warm agar over them on the filter.
When cooled, I peel off the filter, leaving me with a thin layer of cells embedded in the agar. I then cut the agar layer into small blocks to enhance fluid exchange during subsequent treatments. My problem is, the cells are light colored to faintly black, and the thin layer of cells does not stand out well, particularly when in the various stages of epon infiltration/polymerization. I tried adding some azure II blue dye I had on hand to the agar so I could see the agar blocks more easily, but by the time I got up to epon/acetone, the color was gone. Any suggestions for a dye that will withstand both the water based and acetone solutions, or an alternative way to mark the little agar blocks so I can more easily monitor them throughout the process?
Thanks in advance
Steve
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Email: vicenzie-at-si.edu Name: Ed Vicenzi
Organization: Smithsonian Institution
Title-Subject: [Filtered] Senior Scientist, Microscopy & Microanalysis - position available
Message: [16-LM-CR] Senior Scientist, Microscopy & Microanalysis. A Sr. Microscopist will characterize widely diverse samples, including organic and inorganic chemicals, household goods, pharmaceuticals, foods, plastics, etc. Analyst must execute experimental studies and deliver high quality data using a broad range of analytical methods including SEM, EDX, FTIR microscopy, Polarized Light microscopy, Fluorescence, Image analysis. Proficiency with sample preparation methods (sectioning, mounting microtome, etc.) required. Familiarity with GMP/GLP and excellent writing skills. PhD in Analytical Chemistry - 3+ years industrial experience, M.S. -n 5+ years industrial experience. Background in Mineralogy a plus
Please direct questions regarding this position to:
======================== Jonathan Chun, Ph.D. Alliance Technologies 9 Deer Park Dr. Suite B Monmouth Jct., NJ 08852 732-355-1234 (ph) jchun-at-alliancetechgroup.com www.alliancetechgroup.com
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Email: 13qw9-at-queensu.ca Name: Jason
Organization: Queen's University
Title-Subject: [Filtered] TEM FEI low background double tilt holder tip repair
Message: Hi there,
We are encountering an issue with our FEI low background double tilt holder. Probably because of some deformation on the holder tip, now the two small pins cannot hold the sample cradle in position. Does anyone have any experiences in fixing similar holders or know any company that I can contact?
Thanks,
One colleague (Fei) has tried to send the same message with no luck. Not sure what's the reason. Apologizes for letting you receive it twice.
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Hi, I have had luck with fast green added at the 100% ethanol step. I make a saturated fast green solution in ethanol and add a drop or two at the 100% ethanol step. It kept the agar blocks rather colored through subsquent infiltrations and polymerization. Sorry I don't have handy what "saturated" means in terms of mg/mL but if you are interested I could probably dig that up somewhere. Good luck, Tobias
On 1/29/16 8:44 PM, microscopy.listserver-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: sbarlow-at-mail.sdsu.edu } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://www.microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying } please copy both sbarlow-at-mail.sdsu.edu as well as the Microscopy } Listserver } --------------------------------------------------------------------------- } } Email: sbarlow-at-mail.sdsu.edu } Name: Steve Barlow } } Organization: SDSU EM Facility } } Title-Subject: [Filtered] TEM: agar embedding cells } } Message: Some advice please on colorizing agar for embedding cells. In } the past, my osmicated pellets in agar blocks were easily trackable, but } when the cells are in a thin layer the cells don't give much contrast, } especially if not osmicated. I am collecting naked eukaryotic cells in } suspension culture, but have problems bring down the smaller cells by } centrifugation. Cultures containing cells are fixed in aldehyde or } aldehyde/osmium cocktail, collected onto millipore Nucleopore filters, } then held in place by adding warm agar over them on the filter. } } When cooled, I peel off the filter, leaving me with a thin layer of } cells embedded in the agar. I then cut the agar layer into small blocks } to enhance fluid exchange during subsequent treatments. My problem is, } the cells are light colored to faintly black, and the thin layer of } cells does not stand out well, particularly when in the various stages } of epon infiltration/polymerization. I tried adding some azure II blue } dye I had on hand to the agar so I could see the agar blocks more } easily, but by the time I got up to epon/acetone, the color was gone. } Any suggestions for a dye that will withstand both the water based and } acetone solutions, or an alternative way to mark the little agar blocks } so I can more easily monitor them throughout the process? } } Thanks in advance } } Steve } } Login Host: 146.244.234.237 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } }
-- __ ___ ^ ___ ___ Tobias I. Baskin / \ / / \ / \ Professor / / / / \ \ \ Biology Department / __/ /__ /___ \ \ \__ University of Mass. / / / \ \ \ 611 N. Pleasant St. / / / \ \ \ Amherst, Massachusetts / /___ / \ \___/ \_____ USA 413-545-1533 www.bio.umass.edu/biology/baskin
==============================Original Headers============================== 4, 23 -- From baskin-at-bio.umass.edu Mon Feb 1 16:40:07 2016 4, 23 -- Received: from marlin.bio.umass.edu (bio.umass.edu [128.119.55.19]) 4, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u11Me6ng007888 4, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 1 Feb 2016 16:40:07 -0600 4, 23 -- Received: from gouzibyte.bio.mor.nsm (beutopia.bio.umass.edu [128.119.55.10]) 4, 23 -- (authenticated bits=0) 4, 23 -- by marlin.bio.umass.edu (8.14.4/8.14.4/Debian-4.1ubuntu1) with ESMTP id u11MdrUG010762 4, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES128-SHA bits=128 verify=NO); 4, 23 -- Mon, 1 Feb 2016 17:39:53 -0500 4, 23 -- From: Tobias Baskin {baskin-at-bio.umass.edu} 4, 23 -- Subject: Re: [Microscopy] viaWWW:TEM: agar embedding cells 4, 23 -- To: Microscopy-at-microscopy.com, sbarlow-at-mail.sdsu.edu 4, 23 -- References: {201601300144.u0U1irGV011027-at-ns.microscopy.com} 4, 23 -- Message-ID: {56AFDEB9.8020907-at-bio.umass.edu} 4, 23 -- Date: Mon, 1 Feb 2016 17:39:53 -0500 4, 23 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0) 4, 23 -- Gecko/20100101 Thunderbird/38.5.1 4, 23 -- MIME-Version: 1.0 4, 23 -- In-Reply-To: {201601300144.u0U1irGV011027-at-ns.microscopy.com} 4, 23 -- Content-Type: text/plain; charset=windows-1252; format=flowed 4, 23 -- Content-Transfer-Encoding: 7bit 4, 23 -- X-Greylist: Sender succeeded SMTP AUTH, not delayed by milter-greylist-4.3.9 (marlin.bio.umass.edu [128.119.55.19]); Mon, 01 Feb 2016 17:39:53 -0500 (EST) 4, 23 -- X-Scanned-By: MIMEDefang 2.73 ==============================End of - Headers==============================
Not directly answering your question but making a remark:
You are working with a cell monolayer, meaning approx. 50-um thin layer. This is so thin that you don't have to care about fluid exchange, I don't think that cutting your agar in small blocks will add anything. I would keep the whole agar block standing at the bottom of a jar, so you always know that the cells are on the upper side.
Regards Stephane
-------------------------------------------- On Mon, 2/1/16, baskin-at-bio.umass.edu {baskin-at-bio.umass.edu} wrote:
Subject: [Microscopy] Re: viaWWW:TEM: agar embedding cells To: nizets2-at-yahoo.com Date: Monday, February 1, 2016, 11:48 PM
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Hi, -* -*-*-*I have had luck with fast green added at the 100% ethanol step. I make a saturated fast green solution in ethanol and add a drop or two at the 100% ethanol step. It kept the agar blocks rather colored through subsquent infiltrations and polymerization. Sorry I don't have handy what "saturated" means in terms of mg/mL but if you are interested I could probably dig that up somewhere. Good luck, -* -* -* -* -* -* -* -* -* -* -* -*-*-*Tobias
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The Hooke College of Applied Sciences, located in Westmont, IL, is offering its transmission electron microscopy short course April 5-7, 2016. In addition to lectures, this course emphasizes hands-on training using state-of-the-art equipment. For further TEM training details and registration information, please follow the link below:
Best regards- __________________________________________________ Chris Gorman Admissions Specialist Hooke College of Applied Sciences 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) www.hookecollege.com
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Email: h.wu2-at-lboro.ac.uk Name: Houzheng Wu
Organization: Loughborough University UK
Title-Subject: [Filtered] Job opening: Research Associate in Nuclear Graphite
Message: Research Associate in Nuclear Graphite http://www.jobs.ac.uk/job/AMX298/research-associate-in-nuclear-graphite/
Loughborough University - Department of Materials Location: Loughborough, UK Salary: #28,982 to #31,656 per annum Hours: Full Time Contract Type: Contract / Temporary Placed on: 2nd February 2016 Closes: 7th February 2016 Job Ref: REQ15383
Fixed term for 33 months Understanding and Improving Graphite for Advanced Nuclear Fission (UNIGRAF) Required to undertake experimental research on structure from atomic- to micro-scale of iso-graphite for advanced nuclear reactors. The project aims to improve the capability of nuclear graphite through a better understanding of irradiation-induced changes in structure and their impact on mechanical/physical behavior. The post is part of an EPSRC funded project "UNIGRAF" in partnership with Oxford and Bristol University, and multi supporters in USA, China and Germany. Based at Loughborough the post holder will have strong collaborations with scientists/engineers from all partners, and focus on structure characterisation before and after irradiation using HRTEM, EELS, FIB and other techniques. You will have opportunity to access structural characterisation facilities in Oak Ridge National Laboratory, Oxford, and EPSRC National Facility, and multi overseas travel is expected.
A good honours degree (2.1 minimum) and a PhD or equivalent in Materials Science, Physics, or another relevant discipline and experience in materials structural characterisation techniques is essential. Research in graphite/graphene materials and knowledge in nuclear materials is desirable. Application closing date: 7 February 2016
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Email: skmeno1-at-nps.edu Name: Sarath K Menon
Organization: NAVAL POSTGRADUATE SCHOOL
Title-Subject: [Filtered] Electron Microscopy Position at Naval Postgraduate School, Monterey, CA
Message: Research Assistant Professor, AD-1701-03 Department of Mechanical and Aerospace Engineering Naval Postgraduate School, Monterey, CA
The Mechanical and Aerospace Engineering Department of the Naval Postgraduate School is inviting applications from electron Microscopy Specialists to fulfill a Research Faculty position to support the research and development of a variety of materials including metals, ceramics, carbon-based materials and composites. The position will be focused on the operation of several advanced characterization techniques, including fully equipped Scanning Electron Microscope and Transmission Electron Microscope. Responsibilities of the position include the maintenance and upkeep of these complex analytical instruments. The applicant should possess operational expertise with SEM, FIB, TEM lamellae preparation, TEM operation including STEM methods, high resolution TEM and analytical techniques such as EDS, EBSD, EELS and EFTEM. The candidate for the position must be able to perform sample analysis with minimal direction and/or oversight from technical principal investigators. The candidate must also be able to collect, assimilate, and interpret data for technical principal investigators and write technical reports to document results. The position description includes also the coordination of all services required to keep instruments operational, the procurement of all consumables for the microscopy lab and to carry out all the routine maintenance tasks. A PhD in Materials Science or a closely related field is required. A few years of hands-on experience with current state-of-the-art instrumentation and techniques in the area of electron microscopy (SEM, TEM, STEM, EFTEM and FIB) is mandatory. The ability to teach a course related to Advanced Materials Characterization techniques will be highly valued. In addition, the candidate is expected to train Masters level students to use instruments and help them with data analysis. The ability to obtain a US security clearance is mandatory.
Applications should be received by 1 April 2015 and include a curriculum vitae, writing sample, summary of available teaching evaluations, and three letters of recommendation. Please address the applications to:
Garth V. Hobson Professor and Chair Department of Mechanical & Aerospace Engineering 700 Dyer Road, Bldg 245 -n Watkins Hall, WA-338 Naval Postgraduate School Monterey, CA 93943 TEL: 831-656-2586/831-656-2888 www.nps.edu/mae gvhobson-at-nps.edu
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Email: AThiessen-at-wisc.edu Name: Anna Thiessen
Organization: Electron Microscopy Program/ Madison Area Technical College
Title-Subject: [Filtered] Calling all Graduates
Message: Hey Listservers we need your help! The Electron Microscopy Program at Madison Area Technical College in Madison, Wisconsin is closing its door after 24 years. The present and past program directors (Michael Kostrna and Glenn Boda) are trying to organize a reunion/-iwake-i for the program at the end of May. Unfortunately, after 24 years a lot of contact information has been changed and not updated! If you or anyone you know graduated or participated in the program please pass the word on. If you would like to find out more information, please contact Jennifer Kostrna at kostrna.emwakeparty-at-gmail.com.
Many Thanks, Anna Thiessen (Recent Graduate)
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Title-Subject: [Filtered] Free Kevex Sigma EDX System for SEM
Message: TSS has one complete Kevex Sigma EDX system, that needs a good home. Detector (window looks good), collimator, Sigma Analyzer, 4855 Digital Beam Control Interface Module, and cable set. Was installed on XL 30 W SEM.
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Email: debrat-at-bcm.edu Name: Debra M Townley
Organization: Baylor College of Medicine
Title-Subject: [Filtered] Speckled TEM Samples
Message: This is awful - I am having a terrible time getting clean TEM sections. I filter the primary fix mixture with a 0.1um vacuum filtration unit; I've tried changing the water and am currently using RICCA ultra-pure H2O; I have stopped en bloc staining altogether; I use Spurr's Low Viscosity embedding resin. Most recently I have been ethanol-washing all razor blades used during specimen collection; I use a 0.04% FSG quench after fixation (didn't seem to make a difference). The fine speckling is most noticeable in muscle tissue, although I see it in other tissues as well. I just looked at some freshly cut, unstained mouse foot pad muscle yesterday and there were the black speckles all over the place! The specks are tiny, much smaller than 10nm. It almost looks like immuno-gold, except that I haven't done immuno-gold staining in years, and the specks are not rounded as you might expect with gold particles. They don't seem to be clustered at any specific organelle, and they are definitely in the tissue, not on the surface. Someone please help, this is driving me nuts!
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This could be a couple of things. First did you use phosphate buffer? If your concentration was a 100 mM or over, the Ga, osmium and PO4 can precipitate in the tissue. Check unstained sections to confirm. Also make sure all the Ga is rinsed out of tissue before you get to osmium. Are the tissue pieces small? You can also microwave fixative to get better penetration. Be careful with using reduced osmium and then staining muscle. Lipid granules are affected with the post-section staining with UA and Pb. Apparently the lipid material gets lost after staining and rinsing, I have seen this phenomena and sometimes it will contaminate parts of the section. If you are sure it isn't your staining protocol, then it must be the fixation.
Good Luck, Michael Delannoy
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Email: debrat-at-bcm.edu Name: Debra M Townley
Organization: Baylor College of Medicine
Title-Subject: [Filtered] Speckled TEM Samples
Message: This is awful - I am having a terrible time getting clean TEM sections. I filter the primary fix mixture with a 0.1um vacuum filtration unit; I've tried changing the water and am currently using RICCA ultra-pure H2O; I have stopped en bloc staining altogether; I use Spurr's Low Viscosity embedding resin. Most recently I have been ethanol-washing all razor blades used during specimen collection; I use a 0.04% FSG quench after fixation (didn't seem to make a difference). The fine speckling is most noticeable in muscle tissue, although I see it in other tissues as well. I just looked at some freshly cut, unstained mouse foot pad muscle yesterday and there were the black speckles all over the place! The specks are tiny, much smaller than 10nm. It almost looks like immuno-gold, except that I haven't done immuno-gold staining in years, and the specks are not rounded as you might expect with gold particles. They don't seem to be clustered at any specific organelle, and they are definitely in the tissue, not on the surface. Someone please help, this is driving me nuts!
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This could be a couple of things. First did you use phosphate buffer? If your concentration was a 100 mM or over, the Ga, osmium and PO4 can precipitate in the tissue. Check unstained sections to confirm. Also make sure all the Ga is rinsed out of tissue before you get to osmium. Are the tissue pieces small? You can also microwave fixative to get better penetration. Be careful with using reduced osmium and then staining muscle. Lipid granules are affected by the post-section staining with UA and Pb. Apparently the lipid material gets lost after staining and rinsing, I have seen this phenomena and sometimes it will contaminate parts of the section. If you are sure it isn't your staining protocol, then it must be the fixation.
Good Luck, Michael Delannoy
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Email: debrat-at-bcm.edu Name: Debra M Townley
Organization: Baylor College of Medicine
Title-Subject: [Filtered] Speckled TEM Samples
Message: This is awful - I am having a terrible time getting clean TEM sections. I filter the primary fix mixture with a 0.1um vacuum filtration unit; I've tried changing the water and am currently using RICCA ultra-pure H2O; I have stopped en bloc staining altogether; I use Spurr's Low Viscosity embedding resin. Most recently I have been ethanol-washing all razor blades used during specimen collection; I use a 0.04% FSG quench after fixation (didn't seem to make a difference). The fine speckling is most noticeable in muscle tissue, although I see it in other tissues as well. I just looked at some freshly cut, unstained mouse foot pad muscle yesterday and there were the black speckles all over the place! The specks are tiny, much smaller than 10nm. It almost looks like immuno-gold, except that I haven't done immuno-gold staining in years, and the specks are not rounded as you might expect with gold particles. They don't seem to be clustered at any specific organelle, and they are definitely in the tissue, not on the surface. Someone please help, this is driving me nuts!
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thank you for your thorough listing of your processing step... what you omit, what you changed, etc., etc... Nevertheless you ought to tell us about the == buffer vehicle in fixation as well as after fixation == osmication data == processing schedule right after osmium into ascending alcohols, the latter most important to know which concentration you start. Could it be that you face "salt & pepper" artifact? It would be interesting to see an image of your speckles...
Best regards, Wolfgang
Wolfgang MUSS, PhD Member of MSA until 2015 Retired by Dec. 1st, 2015 SALZBURG, AUSTRIA wij.muss-at-aon.at
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This is awful - I am having a terrible time getting clean TEM sections. I filter the primary fix mixture with a 0.1um vacuum filtration unit; I've tried changing the water and am currently using RICCA ultra-pure H2O; I have stopped en bloc staining altogether; I use Spurr's Low Viscosity embedding resin. Most recently I have been ethanol-washing all razor blades used during specimen collection; I use a 0.04% FSG quench after fixation (didn't seem to make a difference). The fine speckling is most noticeable in muscle tissue, although I see it in other tissues as well. I just looked at some freshly cut, unstained mouse foot pad muscle yesterday and there were the black speckles all over the place! The specks are tiny, much smaller than 10nm. It almost looks like immuno-gold, except that I haven't done immuno-gold staining in years, and the specks are not rounded as you might expect with gold particles. They don't seem to be clustered at any specific organelle, and they are definitely in the tissue, not on the surface. Someone please help, this is driving me nuts!
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==============================Original Headers============================== 13, 23 -- From wij.muss-at-aon.at Fri Feb 5 04:40:26 2016 13, 23 -- Received: from bsmtp8.bon.at (bsmtp8.bon.at [213.33.87.20]) 13, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u15AeL6M022046 13, 23 -- for {microscopy-at-microscopy.com} ; Fri, 5 Feb 2016 04:40:21 -0600 13, 23 -- Received: from anwender1976b3 (unknown [93.83.25.98]) 13, 23 -- by bsmtp8.bon.at (Postfix) with ESMTPA id 3pxYD82BkDz5tlR; 13, 23 -- Fri, 5 Feb 2016 11:40:08 +0100 (CET) 13, 23 -- From: "Wolfgang Muss" {wij.muss-at-aon.at} 13, 23 -- To: {debrat-at-bcm.edu} 13, 23 -- Cc: {microscopy-at-microscopy.com} 13, 23 -- References: {201602042005.u14K5SeB022620-at-ns.microscopy.com} 13, 23 -- In-Reply-To: {201602042005.u14K5SeB022620-at-ns.microscopy.com} 13, 23 -- Subject: [Microscopy] Re: Speckled TEM Samples 13, 23 -- Date: Fri, 5 Feb 2016 11:40:59 +0100 13, 23 -- Message-ID: {004201d16001$b3cd8740$1b6895c0$-at-muss-at-aon.at} 13, 23 -- MIME-Version: 1.0 13, 23 -- Content-Type: text/plain; 13, 23 -- charset="iso-8859-1" 13, 23 -- X-Mailer: Microsoft Office Outlook 12.0 13, 23 -- Thread-Index: AdFfqKW5Na3c1P5rRhWKa2WMh1An2wAV/XRw 13, 23 -- Content-Language: de 13, 23 -- Content-Transfer-Encoding: 8bit 13, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u15AeL6M022046 ==============================End of - Headers==============================
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Hello Debra, Thank you for showing us the image with "speckles". I think that the problem might be in lead staining procedure. Please, look at Arvid B. Maunsbach & Bj/drn A. Afzelius "Biomedical Electron Microscopy (Illustrated Methods and Interpretations)" 1999, Pages 207,Aei228 {doi:10.1016/B978-012480610-8/50011-1} for explanation. We had also similar problems with lead carbonate precipitations in the past. Since our technician started to wear a face shield during sections staining with lead citrate the precipitates (contamination) in them almost vanished.
There is a old paper on "How to remove such precipitates from ultrathin sections": Kuo, J. (1980), A simple method for removing stain precipitates from biological sections for transmission electron microscopy. Journal of Microscopy, 120: 221,Aei224. {doi:10.1111/j.1365-2818.1980.tb04140.x}
We tried this approach several times, but the results were not ideal in our hands.
Best regards
Oldrich
-- Oldrich Benada Institute of Microbiology CAS, v.v.i. Videnska 1083 142 20 Prague 4 Czech Republic
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==============================Original Headers============================== 13, 45 -- From benada-at-biomed.cas.cz Fri Feb 5 07:23:53 2016 13, 45 -- Received: from barracuda.biomed.cas.cz (barracuda.biomed.cas.cz [147.231.40.11]) 13, 45 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u15DNptA021087 13, 45 -- for {microscopy-at-microscopy.com} ; Fri, 5 Feb 2016 07:23:52 -0600 13, 45 -- X-ASG-Debug-ID: 1454678618-05011e54256712a0001-4CH8be 13, 45 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) by barracuda.biomed.cas.cz with ESMTP id inb1JH2j2MV5h0Ez (version=TLSv1 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) for {microscopy-at-microscopy.com} ; Fri, 05 Feb 2016 14:23:38 +0100 (CET) 13, 45 -- X-Barracuda-Envelope-From: benada-at-biomed.cas.cz 13, 45 -- X-Barracuda-Effective-Source-IP: mail2.biomed.cas.cz[147.231.40.32] 13, 45 -- X-Barracuda-Apparent-Source-IP: 147.231.40.32 13, 45 -- Received: from u117ob02 (c111jn.mbu.cas.cz [147.231.44.12]) 13, 45 -- (using TLSv1 with cipher AES128-SHA (128/128 bits)) 13, 45 -- (No client certificate requested) 13, 45 -- by mail2.biomed.cas.cz (Postfix) with ESMTPSA id 388CF2C8070 13, 45 -- for {microscopy-at-microscopy.com} ; Fri, 5 Feb 2016 14:23:33 +0100 (CET) 13, 45 -- Date: Fri, 5 Feb 2016 14:23:32 +0100 13, 45 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 13, 45 -- To: {microscopy-at-microscopy.com} 13, 45 -- Subject: RE: [Microscopy] viaWWW:Image of speckled mouse muscle. 13, 45 -- Message-ID: {20160205142332.74306056-at-u117ob02} 13, 45 -- X-ASG-Orig-Subj: RE: [Microscopy] viaWWW:Image of speckled mouse muscle. 13, 45 -- Organization: =?UTF-8?B?TWlrcm9iaW9sb2dpY2vDvSDDunN0YXY=?= AV 13, 45 -- =?UTF-8?B?xIxS?= 13, 45 -- X-Mailer: Claws Mail 3.11.1 (GTK+ 2.24.25; i586-pc-linux-gnu) 13, 45 -- MIME-Version: 1.0 13, 45 -- Content-Type: text/plain; charset=UTF-8 13, 45 -- X-IoP-CAS-MailScanner-ID: 388CF2C8070.773A9 13, 45 -- X-IoP-CAS-MailScanner: Processed 13, 45 -- X-Spam-Status: No 13, 45 -- X-Barracuda-Connect: mail2.biomed.cas.cz[147.231.40.32] 13, 45 -- X-Barracuda-Start-Time: 1454678618 13, 45 -- X-Barracuda-Encrypted: DHE-RSA-AES256-SHA 13, 45 -- X-Barracuda-URL: https://barracuda.biomed.cas.cz:443/cgi-mod/mark.cgi 13, 45 -- X-Barracuda-Scan-Msg-Size: 7668 13, 45 -- X-Virus-Scanned: by bsmtpd at biomed.cas.cz 13, 45 -- X-Barracuda-BRTS-Status: 1 13, 45 -- X-Barracuda-Spam-Score: 1.00 13, 45 -- X-Barracuda-Spam-Status: No, SCORE=1.00 using per-user scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=9.0 tests=BSF_RULE7568M, BSF_RULE_7582B 13, 45 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.26769 13, 45 -- Rule breakdown below 13, 45 -- pts rule name description 13, 45 -- ---- ---------------------- -------------------------------------------------- 13, 45 -- 0.50 BSF_RULE7568M Custom Rule 7568M 13, 45 -- 0.50 BSF_RULE_7582B Custom Rule 7582B 13, 45 -- Content-Transfer-Encoding: 8bit 13, 45 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u15DNptA021087 ==============================End of - Headers==============================
Difficult to say when we don't know your primary buffer / fixation protocol, but I would agree that it looks like it may be due to either precipitate from a phosphate buffer, possibly due to insufficient washing between Ga/OSO4 steps, or osmium 'peppering', usually produced with buffer reactions in the osmium steps, or prolonged fixation in osmium.
I used to have similar problems, and resolved them by changing my buffer to HEPES or cacodylate, extra wash steps between the glutaraldehyde and osmium fixes, and secondary fixing in aqueous osmium as opposed to buffered osmium.
Good luck resolving it - let us know how you get on!
Natalie
Miss Natalie Allcock EM Facility Manager
Electron Microscopy Facility, Centre for Core Biotechnology Services, University of Leicester, University Road, Leicester, LE1 7RH, UK * t: +44 (0)116 2523370 e:* nsa2-at-leicester.ac.uk w: http://www2.le.ac.uk/colleges/medbiopsych/research/cbs/eml/electron-microscopy
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-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: 04 February 2016 20:05 To: Allcock, Natalie S.
X-from: debrat-at-bcm.edu
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Email: debrat-at-bcm.edu Name: Debra M Townley
Organization: Baylor College of Medicine
Title-Subject: [Filtered] Speckled TEM Samples
Message: This is awful - I am having a terrible time getting clean TEM sections. I filter the primary fix mixture with a 0.1um vacuum filtration unit; I've tried changing the water and am currently using RICCA ultra-pure H2O; I have stopped en bloc staining altogether; I use Spurr's Low Viscosity embedding resin. Most recently I have been ethanol-washing all razor blades used during specimen collection; I use a 0.04% FSG quench after fixation (didn't seem to make a difference). The fine speckling is most noticeable in muscle tissue, although I see it in other tissues as well. I just looked at some freshly cut, unstained mouse foot pad muscle yesterday and there were the black speckles all over the place! The specks are tiny, much smaller than 10nm. It almost looks like immuno-gold, except that I haven't done immuno-gold staining in years, and the specks are not rounded as you might expect with gold particles. They don't seem to be clustered at any specific organelle, and they are definitely in the tissue, not on the surface. Someone please help, this is driving me nuts!
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Email: jmhermel-at-inaf.cnrs-gif.fr Name: Hermel
Organization: CNRS
Title-Subject: [Filtered] Leitz Aristoplan microscope detailled service manual
Message: Hi, I should appreciate any help or link to find a Leitz Aristoplan microscope detailled service manual. This microscope has a problem with de centering of lenses in the microscope stand. Thank you very much.
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Email: mlibbee-at-lbl.gov Name: Marissa Libbee
Organization: LBL
Title-Subject: [Filtered] Open Position at the Molecular Foundry
Message: See description below. For more information, please follow the link http://jobs.lbl.gov/open-positions.html
Molecular Foundry Research Scientist-81646 Organization:MS-Materials Sciences Description
Berkeley Lab is Bringing Science Solutions to the World, and YOU can be a part of it!
In the world of science, Lawrence Berkeley National Laboratory (Berkeley Lab) is synonymous with "excellence." That's why we hire the best - whether in research, finance or other operations. This is a great opportunity to bring your top-notch skills to bear in support of world-class scientific research that addresses national and global challenges!
Position Summary: The National Center for Electron Microscopy (NCEM) facility in the Molecular Foundry at Lawrence Berkeley National Laboratory is seeking a Research Scientist to establish an internationally recognized theory and image simulation research program to advance the state of the art in electron microscopy. The primary responsibilities are: 1) to develop theory and methodology for quantitative comparison between theoretical models and data from advanced electron microscopy instruments, and 2) to lead the development of new techniques and instrumentation for electron scattering. This position will require working on a wide range of collaborative research projects in materials characterization and establishment of an outstanding user program and individual research program.
What You Will Do: Develop image processing and simulation tools to enhance capabilities for quantitative data analysis. Develop an independent research program. Lead or participate in the development of new research directions. Offer computational support for ultrahigh resolution microscopy in aberration-corrected instruments in TEM and STEM. Devise new approaches to overcoming limitations of exit wave reconstruction routines and implement these approaches in NCEM-is user program. Refine theory and simulation for STEM imaging to quantify image analysis. Contribute creatively to the development of techniques for atomic resolution tomography. Establish protocols and work flows for the effective management of large data sets for in-house and remote analysis Participate in the design and implementation of new techniques for imaging of energy-related materials, including enhanced visibility and analysis of light elements, imaging at low dose, analysis of defects, and imaging of thick samples. Provide theoretical insight and support as part of a team to develop new detectors, holography, and other instrumentation and techniques. Lead an effort to help close the gap between experimentally available data and theoretically accessible systems. Be familiar with materials modeling and computational procedures for direct comparison with experimental observations. Modeling approaches could range from macroscopic to atomistic methods, from elastic or thermodynamic to kinetic or first principles techniques. Serve as point of contact for the theory and simulation requirements of users related to nanoscale phenomena accessible to electron microscopy imaging. Oversee operation of image analysis and computer lab at NCEM. Provide expertise in the application of existing commercial software and the development of custom modules and scripts. Serve as scientific contact for user proposals. Critically evaluate scientific proposals for feasibility; design research plans and appropriate microscopy experiments to accomplish specific research objectives. Improve quality of the analysis component of user projects by providing theory and simulation support and collaboration. Work closely with other Molecular Foundry staff scientists. Disseminate research results through publications and presentations at national and international conferences. What Is Required: Proven ability to develop and apply computational methods for image analysis, image simulation and advanced codes for modeling of materials behavior. Excellent scientific publications record in relevant areas Well-developed oral and written communication skills. Ability to work effectively within a team and with various levels of internal and external users and staff. Experience in the management and manipulation of large data sets commensurate with high resolution electron microscopy Ph.D. or equivalent experience in the Physical Sciences or Engineering Strong organizational and time management skills. Ability to manage competing priorities, provide quality work within tight time constraints, and deliver projects on schedule. Additional Desired Qualifications: Experience with computational materials modeling such as density functional theory, molecular dynamics, etc. Strong background of work with experimental data and analysis of images from in situ experiments.
Notes: This is a career appointment.
PLEASE NOTE: This job will close Feb 7, at midnight.
This position requires completion of a background check.
Berkeley Lab addresses the world-is most urgent scientific challenges by advancing sustainable energy, protecting human health, creating new materials, and revealing the origin and fate of the universe. Founded in 1931, Berkeley Lab-is scientific expertise has been recognized with 13 Nobel prizes. The University of California manages Berkeley Lab for the U.S. Department of Energy-is Office of Science.
Equal Employment Opportunity: Berkeley Lab is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age, or protected veteran status. Berkeley Lab is in compliance with the Pay Transparency Nondiscrimination Provision under 41 CFR 60-1.4. Click here to view the poster and supplement: "Equal Employment Opportunity is the Law."
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Email: jhyun-at-gatan.com Name: John Hyun
Organization: Gatan, Inc.
Title-Subject: [Filtered] Research and Development Scientist UK Job Opening
Message: Location: Abingdon, Oxfordshire UK
Gatan is an industry leader in the electron microscopy industry and the Research & Development Scientist will work as part of multidisciplinary teams of scientists and development engineers. The role will provide technical input and deliver innovative technology to new product introductions, meeting project schedules and budgets. There will be a requirement for collaboration across all business functions working closely with the Marketing, Manufacturing and Customer Service teams to assure the success of all new product introductions. The building of relationships and working closely with internal and external customers will be a key requirement.
The prime responsibility will be to engage in product development tasks according to Gatan-is product life cycle (PLC) model, a phased process that includes concept and feasibility, design and development, alpha and beta pilot phases leading to product release. Other responsibilities will involve sustaining engineering for mature and legacy products along with the provision of engineering support to Gatan-is production and customer service teams. The product range and technical challenges are diverse and so the ideal candidate will have a broad range of scientific and engineering skills.
The successful candidate will be cable of working semi-autonomously on multiple tasks and must be capable of providing innovative solutions to technical challenges in high technology product design and development. Equally important will be a keen eye for cost effective solutions that are designed for test, manufacture and service. The role will report directly to the Director of Engineering (UK) and some UK and international travel will be required.
For a full description and application instructions, visit: http://www.gatan.com/company/careers/research-and-development-scientist
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We hope someone on the list has a copy (paper or electronic) of the Oxford INCA User Manual. We were given an 80 page INCA Operator Manual document that provides only a cursory explanation of topics like managing the master standards database. For comparison the Aztec manual is 500 pages. Maybe what we are seeking doesn't exist, but if you do have a manual we'd be happy to pay copying or shipping costs.
Any help you can supply would be appreciated.
Cheers Owen -- Owen P Mills Director, Applied Chemical and Morphological Analysis Laboratory Michigan Technological University 1400 Townsend Dr Houghton, MI 49931 906-369-1875 opmills-at-mtu.edu http://mcff.mtu.edu/acmal/
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Title-Subject: [Filtered] TEM - Cryo-ultramicrotomy of rubber sections
Message: Can anyone recommend a technique for collecting rubber sections during cryo-ultramicrotomy that enables the sections to lie flat (i.e. as wrinkle free as possible) on a TEM support grid? I currently collect my sections off the back of my cryoknife using a sucrose droplet, but as the sections warm coming out of the cryochamber on the droplet they contract and wrinkle before being deposited on a TEM grid (with or without carbon coating). Chamber temperature is -120 C. Any ideas are appreciated. Thank you.
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Email: dalia.yablon-at-surfacechar.com Name: Dalia Yablon
Organization: SurfaceChar
Title-Subject: [Filtered] Upcoming AFM/SPM courses in US, Europe
Message: Dear colleagues,
There are several scanning probe microscopy/atomic force microscopy courses that are happening this spring in both the US and Europe:
April 5-7, 2016 Netherlands: Overview of Scanning Probe Microscopy: A hands-on short course covering the technology, basics of operation, and applications. This course has a March 5, 2016 registration deadline. For course details and registration, go to http://www.surfacechar.com/classesschedule.html
May 10-11, 2016 Boston MA: AFM for Characterization of Polymer Materials. A 2-day hands on course focusing on AFM for polymer applications to study polymer structure, morphology, and materials properties. For course details and registration, go to http://www.afmworkshop.com/characterization-of-polymer-materials-afm-training.html
May 22, 2016 Washington DC: Nanoscale Materials Characterization for the Biomedical and Pharmaceutical Industry. For course details and registration, go to http://techconnectworld.com/World2016/workshops/518.html
Sincerely,
Dalia Yablon, Ph.D. SurfaceChar LLC www.surfacechar.com Dalia.yablon-at-surfacechar.com
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if the wrinkling happens during warming of the droplet, could it be because of the section surface characteristics? Adding salt to the sucrose should help if the surface is charged, multivalent ion salts would be more suited than monovalent. However if the surface is not charged but hydrophobic adding salt would probably increase the wrinkling, and in that case adding a hydrophobic component might help, such as serum albumin. Some detergents can do both.
Good luck!
Jan Leunissen Dunedin, NZ
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I currently collect } my sections off the back of my cryoknife using a sucrose droplet, but as } the sections warm coming out of the cryochamber on the droplet they } contract and wrinkle before being deposited on a TEM grid (with or } without carbon coating). Chamber temperature is -120 C. Any ideas are } appreciated. Thank you. } } Login Host: 199.48.24.10 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } -- } =============================================== } } Do not reply to this messages it is from } the Microscopy Listserver NO-REPLY forwarding } system. 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==============================Original Headers============================== 8, 38 -- From leunissen-at-aurion.nl Mon Feb 8 16:06:44 2016 8, 38 -- Received: from nm6-vm7.access.bullet.mail.bf1.yahoo.com (nm6-vm7.access.bullet.mail.bf1.yahoo.com [216.109.114.150]) 8, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u18M6gFP012755 8, 38 -- for {microscopy-at-microscopy.com} ; Mon, 8 Feb 2016 16:06:43 -0600 8, 38 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1454969189; bh=5CcktKX92OvC/tfr4Zhvb2o/3hryqQPIIo0UZ5ZKDDk=; h=Subject:From:In-Reply-To:Date:Cc:References:To:From:Subject; b=IM1I0QyuVljo+ccZqg+K37rcVdQl29WJ9vmFsRgw5hVvDM0iWJ6+SWNm1TTSND+CaT8KgB76B2ZFxSL0R0bl0A2G81ROlWQyuAfVpE4oE/E3vH5i/zAlfDS73UX5E/UcmdI6QDOtG2sj9vfyINRMOgdNpwD8c0sguyFTVuvpmzjcO/pDs9rza9dr18JnKmoWp7HqBcaaxpNSfNCIGGy2rKy4A5UErCcsTO2YAPJayvaM5NQeBlWZ2N3/ulKryIVjqPBg+KNBDOh3o9uB8Rj0iuB9/rYmXsCTVPLb+m4o5DYgiEOAs6wOT6TcnEg3kl3BqlXui/vJAaMHP6/KV5E5Xw== 8, 38 -- Received: from [66.196.81.164] by nm6.access.bullet.mail.bf1.yahoo.com with NNFMP; 08 Feb 2016 22:06:29 -0000 8, 38 -- Received: from [124.108.96.24] by tm10.access.bullet.mail.bf1.yahoo.com with NNFMP; 08 Feb 2016 22:06:29 -0000 8, 38 -- Received: from [127.0.0.1] by omp1001.tnz.mail.aue.yahoo.com with NNFMP; 08 Feb 2016 22:06:28 -0000 8, 38 -- X-Yahoo-Newman-Id: 723320.45878.bm-at-omp1001.tnz.mail.aue.yahoo.com 8, 38 -- Received: (qmail 33870 invoked by uid 1000); 8 Feb 2016 22:06:28 -0000 8, 38 -- Received: from 124.108.96.119 by rel109.mail.aue.yahoo.com with SMTP; Mon, 08 Feb 2016 22:06:28 +0000 8, 38 -- Received: (qmail 30454 invoked from network); 8 Feb 2016 22:06:28 -0000 8, 38 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1454969188; bh=5CcktKX92OvC/tfr4Zhvb2o/3hryqQPIIo0UZ5ZKDDk=; h=Content-Type:Mime-Version:Subject:From:In-Reply-To:Date:Cc:Content-Transfer-Encoding:Message-Id:References:To; b=fiFcNGAtFzcvC1sdBMedTzxosX8EG6cY+3YI964f0JT2geDTyXR3GSixv1w3LGYTF3prV+k4mRW+k8C3SCQgf8eCSaUdy0UkcJX2iZh2wYI4Y3G0PnpHBLURZHVJ1b8etcENiF1d99HI2A2JJRPt/PcRgfMEz/a3qvqb9MnlZ3M= 8, 38 -- X-Yahoo-Newman-Property: ymail-3 8, 38 -- X-YMail-OSG: rq1ahn0VM1kgoVAZKyfIvXbQ_Aqvmwcu8iyyJlT_aTn1_7r 8, 38 -- 7dkY7JiG8Wtvdgs939FPO7d0XoAK1tH.kl2UK64i4axQL4zEX7U_dJgBuL1z 8, 38 -- 4ToOKqlT4iSphgNf5nPpC8idg.PklZliIANd8qPbB_Whhh.O6OAfnz8a9xbC 8, 38 -- tF007IgVq9j95lv2nBEaumiqjIGdaWeH0wBM1hTsFfpY_ko8EIyRA.jMl2nS 8, 38 -- 6mM5MUlu.IcILgop1vS7s9929Cl9jWNNJW0N1xxGvkYCMRvXpTo.6Zie1UWy 8, 38 -- T0QXRv85PUjvVfx6Dz3g89nFtzmMKFqR9BABRiBBUeZX_R6KmcYQrcIa.z0O 8, 38 -- 8UYhreHKLuRS41nY20_BguS0Du.whts1M0.iCOe89Zv0.sRqeWTRTIoVezpA 8, 38 -- eR5e5wCyW_mPuRfCyqkeNDkI1JJNhJqQ5KyMG4z7DQQcU.BHNQN_N07zbBKn 8, 38 -- W6YjLINMnp9r1RkG__CabCeDfvu1zjwDh7ss8KoO1ZqOWzskMjIOsjExv0_w 8, 38 -- b4ok6GhEXr0.hdWn6RrTfD1Jp9yTR7dKYR8Zw5LlP_1_m.ujA 8, 38 -- X-Yahoo-SMTP: E1TINkOswBCOniPaNnp23qBGQpLN4KsU.cr0KdlJlWhF 8, 38 -- Content-Type: text/plain; charset=us-ascii 8, 38 -- Mime-Version: 1.0 (Mac OS X Mail 8.2 \(2104\)) 8, 38 -- Subject: Re: [Microscopy] viaWWW:TEM - Cryo-ultramicrotomy of rubber sections 8, 38 -- From: Jan Leunissen {leunissen-at-aurion.nl} 8, 38 -- In-Reply-To: {201602082041.u18KfFGe021301-at-ns.microscopy.com} 8, 38 -- Date: Tue, 9 Feb 2016 11:06:26 +1300 8, 38 -- Cc: badamianand-at-bfusa.com 8, 38 -- Message-Id: {D8629196-A927-4DF2-BD24-5C1E3F243E1F-at-aurion.nl} 8, 38 -- References: {201602082041.u18KfFGe021301-at-ns.microscopy.com} 8, 38 -- To: microscopy-at-microscopy.com 8, 38 -- X-Mailer: Apple Mail (2.2104) 8, 38 -- Content-Transfer-Encoding: 8bit 8, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u18M6gFP012755 ==============================End of - Headers==============================
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The hotel reservation link is open and the extended deadline for paper submission is less than a week away. As you (or your students) are making plans to attend the Columbus meetings (July 24-28), please don't forget about MSA's student bursary program. Its purpose is to encourage students to attend the meetings by helping to defray some of the costs while giving them an opportunity to meet and interact with the established microscopy community.
The students will be paid $10 an hour to work for ~20 hours during the meeting or pre-meeting events. The jobs involve such things as providing support in the different symposia (assisting with audio-visual needs, speaker set-up, maintaining an attendance count), staffing the volunteer office, and helping with vendor tutorial sign-up. Payment is given as a check at the end of the meetings or when the student leaves Columbus.
Once the program has been finalized, each registered bursary will be contacted and allowed to choose the times and activities they would like to work. Many times they end up "working" sessions they would attend anyway. There is an added bonus of $10 cash to help with meals for each morning and/or afternoon session worked and a meeting shirt.
If anyone would like to participate in the bursary program be sure to check the "I wish to apply for a student bursary" box in section 2 of the registration form (registration opens March 1) and send me an email of your intention. Bursary space is limited, so sign-up early. Applicants for the bursaries must be members of MSA or MAS, enrolled as students at a recognized educational institution, and have paid their registration fee.
For those 'non-students' volunteers are also needed to help with the above mentioned meeting activities. Although not paid on an hourly basis as the student bursaries, volunteers do receive a meeting shirt and the same cash allotment to help with meals. Volunteers also have the opportunity to interact more with the microscopy community as they assist with meeting tasks.
If anyone has any questions about the bursary/volunteer program, or would like to participate, please contact me. Amanda
Amanda Lawrence Institute for Imaging and Analytical Technologies Mississippi State University 662-325-7998 alawrence-at-i2at.msstate.edu
==============================Original Headers============================== 10, 31 -- From ALawrence-at-i2at.msstate.edu Wed Feb 10 14:04:39 2016 10, 31 -- Received: from chokecherry.its.msstate.edu (chokecherry.its.msstate.edu [130.18.2.120]) 10, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1AK4cDQ026751 10, 31 -- for {Microscopy-at-microscopy.com} ; Wed, 10 Feb 2016 14:04:38 -0600 10, 31 -- Received: from mail06.ad.msstate.edu (mail06.ad.msstate.edu [130.18.230.65]) 10, 31 -- by chokecherry.its.msstate.edu (8.13.8/8.13.8) with ESMTP id u1AK4OMZ008327 10, 31 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=FAIL) 10, 31 -- for {Microscopy-at-microscopy.com} ; Wed, 10 Feb 2016 14:04:25 -0600 10, 31 -- X-Sender: {} 10, 31 -- Received: from MAIL02.ad.msstate.edu (2002:8212:e63d::8212:e63d) by 10, 31 -- mail06.ad.msstate.edu (2002:8212:e641::8212:e641) with Microsoft SMTP Server 10, 31 -- (TLS) id 15.0.913.22; Wed, 10 Feb 2016 14:04:21 -0600 10, 31 -- Received: from MAIL02.ad.msstate.edu ([fe80::7846:3039:9492:24b0]) by 10, 31 -- mail02.ad.msstate.edu ([fe80::7846:3039:9492:24b0%13]) with mapi id 10, 31 -- 15.00.0913.011; Wed, 10 Feb 2016 14:04:21 -0600 10, 31 -- From: "Lawrence, Amanda" {ALawrence-at-i2at.msstate.edu} 10, 31 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 10, 31 -- Subject: M&M 2016 student and volunteer meeting assistance 10, 31 -- Thread-Topic: M&M 2016 student and volunteer meeting assistance 10, 31 -- Thread-Index: AdFkPWfcCSeNajF7SCqWm3W6g3+d4g== 10, 31 -- Date: Wed, 10 Feb 2016 20:04:20 +0000 10, 31 -- Message-ID: {4ad3a9b7cfc94ddbb127cf560d67af83-at-mail02.ad.msstate.edu} 10, 31 -- Accept-Language: en-US 10, 31 -- Content-Language: en-US 10, 31 -- X-MS-Has-Attach: 10, 31 -- X-MS-TNEF-Correlator: 10, 31 -- x-originating-ip: [130.18.230.93] 10, 31 -- Content-Type: text/plain; charset="us-ascii" 10, 31 -- MIME-Version: 1.0 10, 31 -- Content-Transfer-Encoding: 8bit 10, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u1AK4cDQ026751 ==============================End of - Headers==============================
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Email: alkoh-at-stanford.edu Name: Ai Leen Koh
Organization: Stanford University
Title-Subject: [Filtered] Stanford Imaging Workshop: Advanced applications of high speed imaging in S/TEM
Message: Friday, March 11, 2016 8:30 am - 5:00 pm Stanford University Stanford Nano Shared Facilities 94305-4088 Stanford , CA
Stanford Nano Shared Facilities (SNSF) invites you to attend this unique S/TEM workshop demonstrating the latest high speed imaging technology and applications specifically for in-situ TEM and 4D STEM diffraction.
This comprehensive, full-day workshop will feature lectures by leading experts, open Q&A sessions, and live microscope demonstrations all in the state-of-the-art SNSF research complex. The microscope sessions will be performed on an aberration (image) corrected, monochromated FEI Titan environmental (S)TEM with a OneView in-situ camera.
This workshop is complimentary to all registered and confirmed participants and includes breakfast, lunch and refreshments. Seating is limited.
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Title-Subject: [Filtered] Microscopy Sales Opportunity- Bay Area
Message: Good Afternoon!
We have recently had a world leading microscopy client open up a few microscopy sales opportunities in the greater Bay Area. The ideal candidate would have an expertise in microscopy and some sales experience.
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Email: Linda.Davis-at-Vesuvius.com Name: Linda L Davis
Organization: Vesuvius Refractories R&D USA
Title-Subject: [Filtered] Leitz Wetzlar Orthoplan largefield microscope to give away
Message: Greetings!!
We have an old but excellent research microscope that has been used almost daily here since 1969, by the same microscopist who just retired after 49 years. It is a fantastic scope with a very large footprint. It is a Leitz Wetzlar Orthoplan, large field microscope. It has been serviced by professional companies almost yearly since purchased new in 1969.
We are replacing it, but I hate to just throw this away. The oculars, objectives, accessories may well be something others can use, if not the entire microscope. It is a museum piece. Again, it has not been sitting idle. Please let me know if you want the entire microscope (VERY heavy for shipping, and likely fragile) or any of the parts. It-is located at our R&D site, halfway between Toledo and Cleveland.
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Email: wambrose-at-unc.edu Name: Wallace Ambrose
Organization: UNC
Title-Subject: [Filtered] ESEM live image to external computer
Message: Hello Listers There is a video out BNC on the back of the FEI Quanta 200 ESEM. In Low Vac mode I can see the signal when connecting to an external view monitor. The signal is horizontal lines that change in size and number when I change image resolution or scan speed. Also B/C seems to adjust normally. What can I do to make this into a usable image signal to use for internet teaching outreach project? Is there a better way to get the SEM signal to the internet?
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You can use Team Viewer or VNC to directly share desktop of your SEM with remote computer(s).
If you want to grab video from BNC then decent video grabber card and some coding/configuring may be needed. I've used Epiphan PCIe in the past, though not with the FEI E-SEM that you have:
http://www.epiphan.com/products/dvi2pcie-duo/
Valery Ray - also with AIM Lab, UMDCP ======================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844, USA Phone: +1-978-296-5063 E-Mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com UMD E-Mail: vray-at-umd.edu
On 2/11/2016 12:05 AM, microscopy.listserver-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: wambrose-at-unc.edu } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying } please copy both wambrose-at-unc.edu as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: wambrose-at-unc.edu } Name: Wallace Ambrose } } Organization: UNC } } Title-Subject: [Filtered] ESEM live image to external computer } } Message: Hello Listers } There is a video out BNC on the back of the FEI Quanta 200 } ESEM. In Low Vac mode I can see the signal when connecting to an } external view monitor. The signal is horizontal lines that change in } size and number when I change image resolution or scan speed. Also B/C } seems to adjust normally. What can I do to make this into a usable image } signal to use for internet teaching outreach project? Is there a better } way to get the SEM signal to the internet? } } Login Host: 152.19.204.249 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- }
==============================Original Headers============================== 7, 33 -- From vray-at-partbeamsystech.com Thu Feb 11 08:23:24 2016 7, 33 -- Received: from nm31-vm2.bullet.mail.bf1.yahoo.com (nm31-vm2.bullet.mail.bf1.yahoo.com [72.30.239.10]) 7, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1BENO4K008372 7, 33 -- for {microscopy-at-microscopy.com} ; Thu, 11 Feb 2016 08:23:24 -0600 7, 33 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1455200591; bh=a0hht1gkvP2BXihI3+fAlT3Serg9gsbcb8sb6jnlRW0=; h=Subject:To:References:From:Date:In-Reply-To:From:Subject; b=spIVg3Pz6s23ESkgkU/CfkhYIzhTxyuyuhmFDdnzO3/cwsPXIQlQwJ7TX0Jnm7cP76hC0+jwu1VEA6suTi1NyNLy1WrSX/phTmdeCjrfMKXjId+qfZ8YnGRDH7NRElBH0O4Zd1glRPTLYKrYF48Gz5HoDNTpk5wq5FOrckQ7EuDgBFjsBirUXgJtVN5iGcktapp9xHbXLHWT/ELLuErxM7tFLSyoGhlkz2VyUJ/4mLFSc4XHX952t8GaAkC8X8UaQK/60miVprKBaXVG8LgmSzKfJrfwaxsw84p+s7raasBjL/EpNMdX1VNkSMaROvNHWm1cBMOy/CWUZ5jm9DabPg== 7, 33 -- Received: from [98.139.170.182] by nm31.bullet.mail.bf1.yahoo.com with NNFMP; 11 Feb 2016 14:23:11 -0000 7, 33 -- Received: from [98.139.211.204] by tm25.bullet.mail.bf1.yahoo.com with NNFMP; 11 Feb 2016 14:23:11 -0000 7, 33 -- Received: from [127.0.0.1] by smtp213.mail.bf1.yahoo.com with NNFMP; 11 Feb 2016 14:23:10 -0000 7, 33 -- X-Yahoo-Newman-Id: 991022.87580.bm-at-smtp213.mail.bf1.yahoo.com 7, 33 -- X-Yahoo-Newman-Property: ymail-3 7, 33 -- X-YMail-OSG: 9E0eSKoVM1miSumfmEVXNofqt9k2.K51w3WRG_lRjyz3Gym 7, 33 -- R.89fGhBDXHaM22vAiSZHei53AggDUWcKAII8wZG19d3nrPfchxTPXGKhvlm 7, 33 -- OWvLVYSCNW20IZKi9SiQ17q1KMG33niWPqG7xzHoN_t5TNqjVc8ktWnURZ.F 7, 33 -- vYRs9pXJMjOnivgJNtcTwqndKqkxM7Ox2t.YzoOfipcUHMHe66H0Ke8vK4Nw 7, 33 -- VLSaxNAbamrMfHHBqSPICtvyMFU5h3lnpzPz35xGcAIjnC8cuG96pQwy_AP9 7, 33 -- iQcYKqnPKc543BhQ1HP63ZCkeoEKjzPHHkO7_ZVDIz.oPt._GU9JOsHX6w7q 7, 33 -- 5h0sszRs1L3tk1J.UArBU7vblwyrqyi5VB5FBRVIFBdAdk08xPxqxgVfm82s 7, 33 -- Egos3j5jZv7JsulTiTZz8bTlQC8Nd.G1PYiwltdOGjpDBwakw9ms7aeb4_Cf 7, 33 -- 4PltEe0pKxRUZekbEa3btwfvlgjPP7D4wnGKZrEa2naMfqOg.wgE5EUyxR_w 7, 33 -- V0E0udJnYM5NN_WPeEI7hAIv21LKGFcl_tKTgbwdooZmyyu6X7r0DYQ-- 7, 33 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 7, 33 -- Subject: Re: [Microscopy] viaWWW:ESEM live image to external computer 7, 33 -- To: microscopy-at-microscopy.com, wambrose-at-unc.edu 7, 33 -- References: {201602110505.u1B55X86010356-at-ns.microscopy.com} 7, 33 -- From: Valery Ray {vray-at-partbeamsystech.com} 7, 33 -- Message-ID: {56BC9950.8020005-at-partbeamsystech.com} 7, 33 -- Date: Thu, 11 Feb 2016 09:23:12 -0500 7, 33 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:38.0) Gecko/20100101 7, 33 -- Thunderbird/38.5.1 7, 33 -- MIME-Version: 1.0 7, 33 -- In-Reply-To: {201602110505.u1B55X86010356-at-ns.microscopy.com} 7, 33 -- Content-Type: text/plain; charset=windows-1252; format=flowed 7, 33 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Hello, I am looking for some advice regarding making graphene TEM grids. I've had some success using a resist-free transfer of graphene from copper foil onto a grid. It is the very last step that is most vexing: lifting the graphene covered grid out of the etching solution. The grid is face-down in the solution, floating on a graphene "raft". Picking it up proves problematic. The filter paper pick-up method does not work because the surface tension of the etching solution is such that the grid "runs away" from the paper. I did grab the grids with tweezers after several tries (same issue), but by then damage had been done to the monolayer. One paper suggested draining the etchant away, but then the I am concerned the grid will be face-down on the bottom of the petri dish. Any helpful ideas?
Regards, Larry Scipioni
Regards, Larry
==============================Original Headers============================== 7, 49 -- From les-at-zsgenetics.com Thu Feb 11 10:48:45 2016 7, 49 -- Received: from gateway24.websitewelcome.com (gateway24.websitewelcome.com [192.185.51.162]) 7, 49 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1BGmjws000462 7, 49 -- for {microscopy-at-microscopy.com} ; Thu, 11 Feb 2016 10:48:45 -0600 7, 49 -- Received: from cm2.websitewelcome.com (cm2.websitewelcome.com [192.185.178.13]) 7, 49 -- by gateway24.websitewelcome.com (Postfix) with ESMTP id 267E0C0CB16BE 7, 49 -- for {microscopy-at-microscopy.com} ; Thu, 11 Feb 2016 10:48:32 -0600 (CST) 7, 49 -- Received: from gator2013.hostgator.com ([50.87.144.13]) 7, 49 -- by cm2.websitewelcome.com with 7, 49 -- id H4oW1s00N0HZY1u014oXb3; Thu, 11 Feb 2016 10:48:32 -0600 7, 49 -- DKIM-Signature: v=1; a=rsa-sha256; q=dns/txt; c=relaxed/relaxed; 7, 49 -- d=zsgenetics.com; s=default; h=Content-Transfer-Encoding:Content-Type: 7, 49 -- MIME-Version:Message-ID:Date:Subject:To:From; 7, 49 -- bh=EB0CfrX1XTC7UT3zDSP7gRDPIuRoS/GS8xium3+Msu0=; b=tI4TWfTrlxGC1CT/40b+Pjp9WG 7, 49 -- qKZdKwRgfU1pt4xgX6L+6vvCVV5ZBtEWd9ETsJvzNAuIkIigWm/o1GJ8LsMrz2l5f77I6yU2udKEn 7, 49 -- 0+9BSldYfuKVDVRxVNlvu4kGUXwttp79HrY9P0gks1/AC491xQnlVki7nu014r6JH84w=; 7, 49 -- Received: from zsgenetics03.z.subnet.rcn.com ([146.115.5.114]:50256 helo=LarryPC) 7, 49 -- by gator2013.hostgator.com with esmtpsa (TLSv1.2:DHE-RSA-AES256-GCM-SHA384:256) 7, 49 -- (Exim 4.86) 7, 49 -- (envelope-from {les-at-zsgenetics.com} ) 7, 49 -- id 1aTuPt-000Deu-OP 7, 49 -- for microscopy-at-microscopy.com; Thu, 11 Feb 2016 10:48:29 -0600 7, 49 -- From: {les-at-zsgenetics.com} 7, 49 -- To: {microscopy-at-microscopy.com} 7, 49 -- Subject: graphene TEM grids 7, 49 -- Date: Thu, 11 Feb 2016 11:48:28 -0500 7, 49 -- Message-ID: {008a01d164ec$08d521a0$1a7f64e0$-at-zsgenetics.com} 7, 49 -- MIME-Version: 1.0 7, 49 -- Content-Type: text/plain; 7, 49 -- charset="us-ascii" 7, 49 -- Content-Transfer-Encoding: 7bit 7, 49 -- X-Mailer: Microsoft Outlook 16.0 7, 49 -- Thread-Index: AdFk7AaR4/9UxD7LRIiWEneUlfWzlQ== 7, 49 -- Content-Language: en-us 7, 49 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report 7, 49 -- X-AntiAbuse: Primary Hostname - gator2013.hostgator.com 7, 49 -- X-AntiAbuse: Original Domain - microscopy.com 7, 49 -- X-AntiAbuse: Originator/Caller UID/GID - [47 12] / [47 12] 7, 49 -- X-AntiAbuse: Sender Address Domain - zsgenetics.com 7, 49 -- X-BWhitelist: no 7, 49 -- X-Source-IP: 146.115.5.114 7, 49 -- X-Exim-ID: 1aTuPt-000Deu-OP 7, 49 -- X-Source: 7, 49 -- X-Source-Args: 7, 49 -- X-Source-Dir: 7, 49 -- X-Source-Sender: zsgenetics03.z.subnet.rcn.com (LarryPC) [146.115.5.114]:50256 7, 49 -- X-Source-Auth: les-at-zsgenetics.com 7, 49 -- X-Email-Count: 22 7, 49 -- X-Source-Cap: enNnYWRtMDE7enNnYWRtMDE7Z2F0b3IyMDEzLmhvc3RnYXRvci5jb20= ==============================End of - Headers==============================
The BNC is definitely the video signal coming out of the Quanta. The question for you is how to get the corresponding X and Y coordinate for the line. You should be able to calculate the line time from the number of pixels and dwell time. You know the number of lines from the pixel count. However, I don't think you will be able to determine where the image starts to get it all in sync.
The D connector next to the BNC is for external control as by an EDS system. I don't know the pin-outs offhand but somebody probably knows them. If you don't have such a system, I don't think I would pursue that route.
I would follow Valery's suggestion of using VNC (or Team Viewer) to monitor the existing screen. You will have to deal with port forwarding since FEI tucks their microscope behind their support computer as a firewall. But they basically do what you need as part of their RAPID support system.
Also, remember that F5 will switch between quadrant and full screen mode. Ctrl-F5 will bring the active quadrant up in full screen on the secondary monitor. I recall there is a way to specify to VNC that that screen should be shared with the rest of the world. It may be that the secondary monitor is what you want to share over the net.
Having said all that, be careful. There is a reason that FEI hid the microscope computer behind a firewall. Make sure your VNC viewers are trustworthy and that they are locked down in view-only mode.
Warren Straszheim
-----Original Message-----
Hi Wallace,
You can use Team Viewer or VNC to directly share desktop of your SEM with remote computer(s).
If you want to grab video from BNC then decent video grabber card and some coding/configuring may be needed. I've used Epiphan PCIe in the past, though not with the FEI E-SEM that you have:
http://www.epiphan.com/products/dvi2pcie-duo/
Valery Ray - also with AIM Lab, UMDCP } X-from: wambrose-at-unc.edu } } This Question/Comment was submitted to the Microscopy Listserver using } the WWW based Form at http://www.microscopy.com/MLFormMail.html } ---------------------------------------------------------------------- } ----- Remember this posting is most likely not from a Subscriber, so } when replying } please copy both wambrose-at-unc.edu as well as the Microscopy Listserver } ---------------------------------------------------------------------- } ----- } } Email: wambrose-at-unc.edu } Name: Wallace Ambrose } } Organization: UNC } } Title-Subject: [Filtered] ESEM live image to external computer } } Message: Hello Listers } There is a video out BNC on the back of the FEI Quanta 200 ESEM. In } Low Vac mode I can see the signal when connecting to an external view } monitor. The signal is horizontal lines that change in size and number } when I change image resolution or scan speed. Also B/C seems to adjust } normally. What can I do to make this into a usable image signal to use } for internet teaching outreach project? Is there a better way to get } the SEM signal to the internet? } } Login Host: 152.19.204.249 } Listserver Email Form V - 20120416 } ----------------------------------------------------------------------
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From donnedua0-at-gmail.com Thu Feb 11 18:10:19 2016 Return-Path: {donnedua0-at-gmail.com} Received: from gmail.com ([210.126.22.123]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id u1C0AFJ2027034 for {microscopylistserverarchive5-at-microscopy.com} ; Thu, 11 Feb 2016 18:10:17 -0600 Message-ID: {64499D9D.F71C185F-at-gmail.com}
X-from: kaszas.1-at-osu.edu
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Email: kaszas.1-at-osu.edu Name: Andrea Kaszas
Organization: Ohio State University-OARDC
Title-Subject: [Filtered] Protocol for embedding rubber in plastic resin
Message: Anybody has a protocol for embedding rubber samples in plastic resin? Not for cryosectioning. How to prepare the rubber for it, for example hardening the rubber somehow? Any suggestion would be greatly appreciated.
Andrea Kaszas Microscopy technician OSU-OARDC
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Dear Colleagues,
We would like to draw your attention to the upcoming conference on super-resolution light microscopy, hosted at the University of Basel in Switzerland on June 7-11, 2016.
Join world-changing thinkers and innovators from the Biophysical and Life Science communities: Super-resolution imaging methods now can provide spatial resolution that is well below the diffraction limit, approaching virtually molecular resolution. They can be applied to biological samples and provide new and exciting views on the structural organization of cells and the dynamics of biomolecular assemblies on wide timescales. These revolutionary developments come with novel requirements for fluorescent probes, labeling techniques, and data interpretation strategies. Researchers from across the scientific spectrum are involved in designing improved tools and solving previously intractable problems using super-resolution techniques.
We invite you to attend the ,AeoInternational Conference On Nanoscopy 2016,Aeo, which will uniquely and exclusively focus on this topic. Held in Basel, Switzerland 07-10 June 2016, it will bring together an international group of experts to discuss the latest advances and future directions in the field.
Markus Sauer, Conference Chair, University of W/orzburg, Germany
Details and registration are on http://www.icon-europe.org
Speakers include Eric Betzig (Janelia, USA) Lothar Schermelleh (Oxford University, UK) Rainer Heintzmann (University Jena, Germany) Ingo Gregor (University of G/dttingen, Germany) Thomas Huser (University Bielefeld, Germany) Markus Sauer (University W/orzburg, Germany) Adriaan Houtsmuller (Erasmus MC, Netherlands) Jonas Ries (EMBL, Germany) Sjoerd Stallinga (TU Delft, Netherlands) Melike Lakadamyali (ICFO, Spain) Suliana Manley (EPFL, Switzerland) Joerg Bewersdorf (Yale, USA) Katrin Willig (MPI EM, Germany) Christian Eggeling (Oxford University, UK) Ilaria Testa (KTH, Sweden) Martin Booth (Oxford University, UK) Erik Manders (UvA, Netherlands) Edoardo Charbon (TU Delft, Netherlands) Oliver Biehlmaier (University Basel, Switzerland) and others.
Another 12 presentations will be selected from submitted participant abstracts.
On behalf of the organizing committee, Gregor Drummen, Oliver Biehlmaier, Henning Stahlberg, and Manuela Holzer
with best greetings, Henning.
Henning Stahlberg, PhD Prof. for Structural Biology, C-CINA, Biozentrum, University Basel Mattenstrasse 26 | D-BSSE | WRO-1058 | CH-4058 Basel | Switzerland http://c-cina.org | Tel. +41-61-387 32 62
==============================Original Headers============================== 12, 40 -- From henning.stahlberg-at-unibas.ch Sat Feb 13 04:22:08 2016 12, 40 -- Received: from mx2-priv.urz.unibas.ch (mx2-priv.urz.unibas.ch [131.152.226.165]) 12, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1DALurG024691 12, 40 -- for {Microscopy-at-microscopy.com} ; Sat, 13 Feb 2016 04:21:59 -0600 12, 40 -- Received: from localhost (localhost [127.0.0.1]) 12, 40 -- by mx2-priv.urz.unibas.ch (Postfix) with ESMTP id 19D5E220164; 12, 40 -- Sat, 13 Feb 2016 11:21:39 +0100 (CET) 12, 40 -- X-Virus-Scanned: amavisd-new at unibas.ch 12, 40 -- Received: from mx2-priv.urz.unibas.ch ([131.152.226.165]) 12, 40 -- by localhost (mx2-mgnt.urz.unibas.ch [127.0.0.1]) (amavisd-new, port 10024) 12, 40 -- with LMTP id KA5rUja9CUoh; Sat, 13 Feb 2016 11:21:39 +0100 (CET) 12, 40 -- Received: from URZ-HT-CAS-1.urz.unibas.ch (urz-ht-cas-1.urz.unibas.ch [131.152.8.131]) 12, 40 -- by mx2-priv.urz.unibas.ch (Postfix) with ESMTPS id 097BE22015E; 12, 40 -- Sat, 13 Feb 2016 11:21:39 +0100 (CET) 12, 40 -- Received: from URZ-MBX-1.urz.unibas.ch ([131.152.8.141]) by 12, 40 -- URZ-HT-CAS-1.urz.unibas.ch ([fe80::f8bd:7aba:d322:ad34%12]) with mapi id 12, 40 -- 14.03.0266.001; Sat, 13 Feb 2016 11:21:38 +0100 12, 40 -- From: Henning Stahlberg {henning.stahlberg-at-unibas.ch} 12, 40 -- To: Microscopy {Microscopy-at-microscopy.com} 12, 40 -- CC: Gregor Drummen {gpcdrummen-at-bionano-solutions.de} , 12, 40 -- Oliver Biehlmaier 12, 40 -- {oliver.biehlmaier-at-unibas.ch} , 12, 40 -- Manuela Holzer {manuela.holzer-at-unibas.ch} 12, 40 -- Subject: ICON 2016 - International Conference on Nanoscopy. Basel, June 12, 40 -- 7-11, 2016. Registration now open. 12, 40 -- Thread-Topic: ICON 2016 - International Conference on Nanoscopy. Basel, June 12, 40 -- 7-11, 2016. Registration now open. 12, 40 -- Thread-Index: AQHRZkhS+E7QZ+G7aE25OZYrqmR1Rw== 12, 40 -- Date: Sat, 13 Feb 2016 10:21:38 +0000 12, 40 -- Message-ID: {23434275-3A85-46D6-8712-52E4A27A09B6-at-unibas.ch} 12, 40 -- Accept-Language: en-US, de-CH 12, 40 -- Content-Language: en-US 12, 40 -- X-MS-Has-Attach: 12, 40 -- X-MS-TNEF-Correlator: 12, 40 -- x-originating-ip: [131.152.226.242] 12, 40 -- Content-Type: text/plain; charset="utf-8" 12, 40 -- Content-ID: {31B4E6794420F14FA05CBC4CD0A31CC4-at-zuv.ads.unibas.ch} 12, 40 -- MIME-Version: 1.0 12, 40 -- Content-Transfer-Encoding: 8bit 12, 40 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id u1DALurG024691 ==============================End of - Headers==============================
Trying to fix one up for personal use. Would be very interested if someone has a manual or can direct me where I might find one. I'm currently following a lead with Pumping Station 1 but looking at other options in case I don't hear back. Thanks!
John
==============================Original Headers============================== 3, 46 -- From johndmcmaster-at-gmail.com Sun Feb 14 23:58:53 2016 3, 46 -- Received: from mail-pa0-f42.google.com (mail-pa0-f42.google.com [209.85.220.42]) 3, 46 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1F5wqvm018810 3, 46 -- for {Microscopy-at-microscopy.com} ; Sun, 14 Feb 2016 23:58:53 -0600 3, 46 -- Received: by mail-pa0-f42.google.com with SMTP id fy10so40839592pac.1 3, 46 -- for {Microscopy-at-microscopy.com} ; Sun, 14 Feb 2016 21:58:39 -0800 (PST) 3, 46 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 46 -- d=gmail.com; s=20120113; 3, 46 -- h=to:from:subject:message-id:date:user-agent:mime-version 3, 46 -- :content-type:content-transfer-encoding; 3, 46 -- bh=YbyWQO2HBy9fF4OCLd6NtFtmMVdarZaqOrQofgfF8yk=; 3, 46 -- b=y2DEAnwRQUR1Ay10UX18b7d4moUB2PGi+CYaqjanIjcpqm6OltC7XYJEVda4AFCuK1 3, 46 -- n3VlbKcncu+Mjp7tQoTroTyrt2O3MsAgCjrapZM6gORHd0W2m87GHcpEA8uhkP+J2i4b 3, 46 -- jpfTra8XrYpGONpsm26HR3cBRb7yfo9pThzRDDxvxo2F1YM8pvAYws/ezgF4IvbwnAk3 3, 46 -- TZiP/R00Yaixrn4Mbj/dqJ/rmFld/+ArvBf8AMiOHqr4BBsJeXH2IldEcuj1uXS16Mku 3, 46 -- IimMk9Ck+rG+oJTson0DY7pp/KQMz1XpK4aeYf2gwqCbws0SVcNxJwUagL0ov1lFkE54 3, 46 -- kpiQ== 3, 46 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 46 -- d=1e100.net; s=20130820; 3, 46 -- h=x-gm-message-state:to:from:subject:message-id:date:user-agent 3, 46 -- :mime-version:content-type:content-transfer-encoding; 3, 46 -- bh=YbyWQO2HBy9fF4OCLd6NtFtmMVdarZaqOrQofgfF8yk=; 3, 46 -- b=AZ7MHceWb7ouFp2CqIRby8GWD6ZQGsYy6bDiqtSWgB4pEtTYEdG+z757YhmL1wLu8f 3, 46 -- 6rnJ6qmG+0ho6ar880oaYShAhdlYQ8fJRXK2JmdU1gD48S8HfVfHJvNXOFWw2iFbocvF 3, 46 -- oMZc2Kcb0v7Usop68w27Ggw9Q2ZPnUbU4UXLg3mWxvoGwxIQxMV7ffu+r0WF/8Bsl4XT 3, 46 -- w7xhXCgdHk8pItzLpI9MxNiOh67tpHzY1vpzSzm+ZfbnVQ7W/2m4FpJS7xhQ6leVErd0 3, 46 -- HsTSt4B8A3KCTD5eE0t2sswbJFJqP8ayH+Z+vBCJud0LtddXJ9Qvhmg0aGvGK3wVs/au 3, 46 -- PE0w== 3, 46 -- X-Gm-Message-State: AG10YOQliphydnnz9UHSs0/Kt3LS4KorgkuOJFJ2+exiWH5H5tD/XdbgIc5n0ip4zr9tWw== 3, 46 -- X-Received: by 10.66.219.71 with SMTP id pm7mr20818929pac.137.1455515919016; 3, 46 -- Sun, 14 Feb 2016 21:58:39 -0800 (PST) 3, 46 -- Received: from [192.168.4.218] (c-24-6-49-99.hsd1.ca.comcast.net. [24.6.49.99]) 3, 46 -- by smtp.googlemail.com with ESMTPSA id k14sm35302959pfj.0.2016.02.14.21.58.38 3, 46 -- for {Microscopy-at-microscopy.com} 3, 46 -- (version=TLSv1/SSLv3 cipher=OTHER); 3, 46 -- Sun, 14 Feb 2016 21:58:38 -0800 (PST) 3, 46 -- To: Microscopy-at-microscopy.com 3, 46 -- From: John McMaster {johndmcmaster-at-gmail.com} 3, 46 -- Subject: Wanted: Technics Hummer II manual 3, 46 -- Message-ID: {56C1690D.7050304-at-gmail.com} 3, 46 -- Date: Sun, 14 Feb 2016 21:58:37 -0800 3, 46 -- User-Agent: Mozilla/5.0 (X11; Linux x86_64; rv:38.0) Gecko/20100101 3, 46 -- Thunderbird/38.5.1 3, 46 -- MIME-Version: 1.0 3, 46 -- Content-Type: text/plain; charset=utf-8; format=flowed 3, 46 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Message: The South Carolina INBRE Program and the USC School of Medicine Instrumentation Resource Facility are pleased to announce the 11th annual workshop on Basic Confocal Microscopy. The Workshop will be held June 13-17, 2016 at the USC School of Medicine in Columbia, SC.
Workshop material is directed towards beginning and intermediate users of confocal microscopes and involves a series of lectures (specimen preparation, labeling strategies, proper set-up of instrument operating parameters, proper handling of 2D and 3D confocal images in programs such as Adobe Photoshop and AMIRA), hands on specimen preparation, and time on a number of different point scanning and spinning disk confocal microscopes.
Companies that have provided equipment and applications experts for past workshops include Leica, Nikon, Olympus, Perkin Elmer, Zeiss, Intelligent Imaging Innovations (3i) and Photometrix. Participants will have ample time for hands on use of the instruments and processing of images in Photoshop and AMIRA during the workshop.
Faculty will include Dr. Jay Jerome of Vanderbilt University, Dr. Ralph Albrecht of the University of Wisconsin Madison, Dr. John Mackenzie of North Carolina State University, Dr. Tom Trusk of the Medical University of South Carolina, and Dr. Bob Price of the University of South Carolina.
The $350 tuition covers supplies for processing of specimens, breakfast and lunch for the week, 2 dinners and a reception on Lake Murray.
For registration information please see: http://irf.med.sc.edu/ or contact Anna Harper (Anna.Harper-at-uscmed.sc.edu) or Bob Price (bob.price-at-uscmed.sc.edu)
Bob Price Tel: 803-216-3824 Admin Asst Tel: 803-216-3825 Dept Cell Biology and Anatomy School of Medicine Univ South Carolina 6439 Garner's Ferry Road Columbia, SC 29208
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I am in the process of preparing a grant and I am considering adding about a dozen compound microscopes with phase contrast capabilities 4x-100x objectives. These microscopes would be used in undergraduate teaching labs, so high resolution is unnecessary.
I am unfamiliar with the quality of AmScope microscopes but they have what looks like a decent scope (Model B690B-PL-PCT200INF). We currently have an army of Olympus BH-2s but the number of biology majors has increased significantly, so we are looking for some affordable options.
Does anyone have experience with these microscopes? Any insight would be greatly appreciated!
Thank you,
-Blanca
----------------------------------------- Blanca Carbajal Gonzalez, M.S. Director of Microscopy Science Center 50 College St Mount Holyoke College Clapp Laboratory 123 Office: 413-538-3118 Cell: 559-905-7138 bicarbaj-at-mtholyoke.edu MHC Microscopy
anybody ou there who can help me out with an operation manual in PDF on the 5900? I am also looking for a manual concerning the functions which can be remoted on the 5900. Does anybody have experience doing this? What SEM parameters can be read / written...?
Thanks, Stefan
--
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Do any of you have experience with the newer benchtop or desktop SEM's? They seem quite capable and their cost to purchase and maintain, compared to the full scale SEM's, looks attractive. Their advertised performance is appealing. Do they maintain that performance? Do they require much service? Should I have a service contract? Do the accessories function well (e.g. rotating and tilting stage/holder, charge reduction holder)? Is the provided EDS system comprehensive and reliable? Your replies would be greatly appreciated.
Tom Kremer tkremer-at-ipstesting.com
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we have a SIS Megaview III camera (SIS, then Olympus and now I see it is EMSIS "again") for over 10 years, a real workhorse, always performing well without complaining. Because it works so well I tend to forget about it and now I am just wondering if I need to update the gain and bias corrections (the correction pictures)? I can't remember the last time I did it but it is a long time ago. I know that it is not a lot of work to do it, I just wondered if there is any reason to do this regularly and if yes, what are the time intervals. Many thanks in advance.
Regards Stephane
==============================Original Headers============================== 3, 29 -- From nizets2-at-yahoo.com Thu Feb 18 04:08:18 2016 3, 29 -- Received: from nm14-vm0.bullet.mail.bf1.yahoo.com (nm14-vm0.bullet.mail.bf1.yahoo.com [98.139.213.164]) 3, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1IA8HGp022220 3, 29 -- for {microscopy-at-microscopy.com} ; Thu, 18 Feb 2016 04:08:17 -0600 3, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1455790083; bh=x7ccEl93SQjy6RmTa7YGFTI9AoSGhaKtVxThqGCF8uI=; h=Date:From:Reply-To:To:Subject:References:From:Subject; b=mxmCwIJ35ltTrPD3kub/zVxqaafRcOAxfgm6ezzoJrp1pUCYmcfPSQRcz2UZCYB7E2VDYutG4Acwoc6fb+dgYjGb/qn0SYRRjmHssI4OgoU5KzEsVT7z/hXuvkawJQrdxcMBZFjaSBsgwQW/NqldgKsdDF7QxPC3nogGqNgnXcC8R6tuyu5iftY8Mv4hEEhxZBqjkVbYVTJD4II/3DuBYpGu2M8zgAudq87whg7+Fz8BK1DwDQbxqX6RVf7LeWJ6wCuaLj+MlbtnyWJgVCLjFByywNR82D973IPAXq8MANbIjz9vlXDO0aK/VOoD3HTLmooRkrHVEPifDqHg+3oWrw== 3, 29 -- Received: from [98.139.215.143] by nm14.bullet.mail.bf1.yahoo.com with NNFMP; 18 Feb 2016 10:08:03 -0000 3, 29 -- Received: from [98.139.212.220] by tm14.bullet.mail.bf1.yahoo.com with NNFMP; 18 Feb 2016 10:08:03 -0000 3, 29 -- Received: from [127.0.0.1] by omp1029.mail.bf1.yahoo.com with NNFMP; 18 Feb 2016 10:08:03 -0000 3, 29 -- X-Yahoo-Newman-Property: ymail-3 3, 29 -- X-Yahoo-Newman-Id: 741770.20773.bm-at-omp1029.mail.bf1.yahoo.com 3, 29 -- X-YMail-OSG: 9nq693UVM1nU.BaDUcqJJgdhtWG7s9wmJYCi2MeGsNslachC50ErOqvae9Gx8Ka 3, 29 -- 9zbrt5rq.gYAwbe3oLBiRmOMyG.BYkEej7Am1ukFXfhrPI_nmsIA4VEGAF51eLmld4zOtlbFAkFv 3, 29 -- U0L7c8ugwikXIHKGOHBVEauDmO7yx579GW9GdgZMgsvNawS1.8XXpys4zbpaG3N0hvBLDNqQ8cyj 3, 29 -- 1TEsA7jPiu6pxVkQ0AaY.w_9g8PB9XnyXBH.mz3Jp5TR9rQoxU7sHPwPjy_JqJd3m83fyNFhvPuQ 3, 29 -- m3dVQ1abp.DifVvCA3Yb4FRYqIDZCOPX0xg8XKxpyzpYnmBp69hrSrCfj_6Snc8sD6UU9NOxJ93f 3, 29 -- dJeWO1GznuG5eY4hKCAH0vmlMa8agqeM.M4tSZaIXP09yXCgaEMSAj.aDIpN4kUvtoJYN9MczS3D 3, 29 -- GJoW6ohYzYMk05lp.BH7g8D8g_6wqQxWiS08xJAMwxRaDjlVrDb41LV9KtUsHqodh4TQT3Z2DqFT 3, 29 -- XNnKGXA-- 3, 29 -- Received: by 66.196.80.114; Thu, 18 Feb 2016 10:08:03 +0000 3, 29 -- Date: Thu, 18 Feb 2016 10:08:02 +0000 (UTC) 3, 29 -- From: Stephane Nizet {nizets2-at-yahoo.com} 3, 29 -- Reply-To: Stephane Nizet {nizets2-at-yahoo.com} 3, 29 -- To: {microscopy-at-microscopy.com} 3, 29 -- Message-ID: {198161144.6166998.1455790082942.JavaMail.yahoo-at-mail.yahoo.com} 3, 29 -- Subject: bias and gain correction on TEM cameras 3, 29 -- MIME-Version: 1.0 3, 29 -- Content-Type: text/plain; charset=UTF-8 3, 29 -- Content-Transfer-Encoding: 7bit 3, 29 -- References: {198161144.6166998.1455790082942.JavaMail.yahoo.ref-at-mail.yahoo.com} ==============================End of - Headers==============================
Hello Stephane, since the blacklevel ("noise") and gain images are a necessary step for getting an artefact-free and evenly background I think you should do this short procedure (depends what version of Analysis or ITEM software you are using) often. When I am installing or updating cameras at the customerYens site I ask for the most used magnfication / spotsize / apertures setting they use and do the correction images with this setting. But: I also know a customer who is doing these image sets prior to each high-res image he uses for publication (makes sense ;-) ).
Best wishes, Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Am 18.02.16 um 11:27 schrieb nizets2-at-yahoo.com: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Listers, } } we have a SIS Megaview III camera (SIS, then Olympus and now I see it is EMSIS "again") for over 10 years, a real workhorse, always performing well without complaining. } Because it works so well I tend to forget about it and now I am just wondering if I need to update the gain and bias corrections (the correction pictures)? } I can't remember the last time I did it but it is a long time ago. } I know that it is not a lot of work to do it, I just wondered if there is any reason to do this regularly and if yes, what are the time intervals. } Many thanks in advance. } } Regards } Stephane } } ==============================Original Headers============================== } 3, 29 -- From nizets2-at-yahoo.com Thu Feb 18 04:08:18 2016 } 3, 29 -- Received: from nm14-vm0.bullet.mail.bf1.yahoo.com (nm14-vm0.bullet.mail.bf1.yahoo.com [98.139.213.164]) } 3, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1IA8HGp022220 } 3, 29 -- for {microscopy-at-microscopy.com} ; Thu, 18 Feb 2016 04:08:17 -0600 } 3, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1455790083; bh=x7ccEl93SQjy6RmTa7YGFTI9AoSGhaKtVxThqGCF8uI=; h=Date:From:Reply-To:To:Subject:References:From:Subject; b=mxmCwIJ35ltTrPD3kub/zVxqaafRcOAxfgm6ezzoJrp1pUCYmcfPSQRcz2UZCYB7E2VDYutG4Acwoc6fb+dgYjGb/qn0SYRRjmHssI4OgoU5KzEsVT7z/hXuvkawJQrdxcMBZFjaSBsgwQW/NqldgKsdDF7QxPC3nogGqNgnXcC8R6tuyu5iftY8Mv4hEEhxZBqjkVbYVTJD4II/3DuBYpGu2M8zgAudq87whg7+Fz8BK1DwDQbxqX6RVf7LeWJ6wCuaLj+MlbtnyWJgVCLjFByywNR82D973IPAXq8MANbIjz9vlXDO0aK/VOoD3HTLmooRkrHVEPifDqHg+3oWrw== } 3, 29 -- Received: from [98.139.215.143] by nm14.bullet.mail.bf1.yahoo.com with NNFMP; 18 Feb 2016 10:08:03 -0000 } 3, 29 -- Received: from [98.139.212.220] by tm14.bullet.mail.bf1.yahoo.com with NNFMP; 18 Feb 2016 10:08:03 -0000 } 3, 29 -- Received: from [127.0.0.1] by omp1029.mail.bf1.yahoo.com with NNFMP; 18 Feb 2016 10:08:03 -0000 } 3, 29 -- X-Yahoo-Newman-Property: ymail-3 } 3, 29 -- X-Yahoo-Newman-Id: 741770.20773.bm-at-omp1029.mail.bf1.yahoo.com } 3, 29 -- X-YMail-OSG: 9nq693UVM1nU.BaDUcqJJgdhtWG7s9wmJYCi2MeGsNslachC50ErOqvae9Gx8Ka } 3, 29 -- 9zbrt5rq.gYAwbe3oLBiRmOMyG.BYkEej7Am1ukFXfhrPI_nmsIA4VEGAF51eLmld4zOtlbFAkFv } 3, 29 -- U0L7c8ugwikXIHKGOHBVEauDmO7yx579GW9GdgZMgsvNawS1.8XXpys4zbpaG3N0hvBLDNqQ8cyj } 3, 29 -- 1TEsA7jPiu6pxVkQ0AaY.w_9g8PB9XnyXBH.mz3Jp5TR9rQoxU7sHPwPjy_JqJd3m83fyNFhvPuQ } 3, 29 -- m3dVQ1abp.DifVvCA3Yb4FRYqIDZCOPX0xg8XKxpyzpYnmBp69hrSrCfj_6Snc8sD6UU9NOxJ93f } 3, 29 -- dJeWO1GznuG5eY4hKCAH0vmlMa8agqeM.M4tSZaIXP09yXCgaEMSAj.aDIpN4kUvtoJYN9MczS3D } 3, 29 -- GJoW6ohYzYMk05lp.BH7g8D8g_6wqQxWiS08xJAMwxRaDjlVrDb41LV9KtUsHqodh4TQT3Z2DqFT } 3, 29 -- XNnKGXA-- } 3, 29 -- Received: by 66.196.80.114; Thu, 18 Feb 2016 10:08:03 +0000 } 3, 29 -- Date: Thu, 18 Feb 2016 10:08:02 +0000 (UTC) } 3, 29 -- From: Stephane Nizet {nizets2-at-yahoo.com} } 3, 29 -- Reply-To: Stephane Nizet {nizets2-at-yahoo.com} } 3, 29 -- To: {microscopy-at-microscopy.com} } 3, 29 -- Message-ID: {198161144.6166998.1455790082942.JavaMail.yahoo-at-mail.yahoo.com} } 3, 29 -- Subject: bias and gain correction on TEM cameras } 3, 29 -- MIME-Version: 1.0 } 3, 29 -- Content-Type: text/plain; charset=UTF-8 } 3, 29 -- Content-Transfer-Encoding: 7bit } 3, 29 -- References: {198161144.6166998.1455790082942.JavaMail.yahoo.ref-at-mail.yahoo.com} } ==============================End of - Headers============================== }
==============================Original Headers============================== 8, 25 -- From stefan.diller-at-t-online.de Thu Feb 18 04:40:43 2016 8, 25 -- Received: from mailout01.t-online.de (mailout01.t-online.de [194.25.134.80]) 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1IAefMr004533 8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 18 Feb 2016 04:40:42 -0600 8, 25 -- Received: from fwd11.aul.t-online.de (fwd11.aul.t-online.de [172.20.27.152]) 8, 25 -- by mailout01.t-online.de (Postfix) with SMTP id 3A8F9427EE 8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 18 Feb 2016 11:40:27 +0100 (CET) 8, 25 -- Received: from mac-pro.fritz.box (ESUE6aZ-whYwAfSldbPQXTZ55PEafbWxBPK4eG+X+EOX9+4smi90B-sXFMUQ+k6wwJ-at-[91.58.167.160]) by fwd11.t-online.de 8, 25 -- with (TLSv1.2:ECDHE-RSA-AES256-SHA encrypted) 8, 25 -- esmtp id 1aWM0T-1m0f680; Thu, 18 Feb 2016 11:40:21 +0100 8, 25 -- Subject: Re: [Microscopy] bias and gain correction on TEM cameras 8, 25 -- To: microscopy-at-microscopy.com 8, 25 -- References: {201602181027.u1IARO2S027682-at-ns.microscopy.com} 8, 25 -- From: Stefan Diller {stefan.diller-at-t-online.de} 8, 25 -- Message-ID: {56C59F94.3010105-at-t-online.de} 8, 25 -- Date: Thu, 18 Feb 2016 11:40:20 +0100 8, 25 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0) 8, 25 -- Gecko/20100101 Thunderbird/38.6.0 8, 25 -- MIME-Version: 1.0 8, 25 -- In-Reply-To: {201602181027.u1IARO2S027682-at-ns.microscopy.com} 8, 25 -- Content-Type: text/plain; charset=windows-1252; format=flowed 8, 25 -- X-ID: ESUE6aZ-whYwAfSldbPQXTZ55PEafbWxBPK4eG+X+EOX9+4smi90B-sXFMUQ+k6wwJ 8, 25 -- X-TOI-MSGID: a7f85412-8967-40de-94d5-f69dc661289f 8, 25 -- Content-Transfer-Encoding: 8bit 8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u1IAefMr004533 ==============================End of - Headers==============================
Hi Stephane, We have SIS MegaView II 16 years old, even older then yours Megaview III. Well, we used to record the correction images quite frequently, approximately once per month or two. As the ccd chip and scintillator were getting older, the interval for "shading correction" adjustment had to be shorter. After 15 years, we had to record every week a new set of gain and bias images.
You can quite easily check whether you need to perform new adjustment of "shading correction" or not. 1. Take-off the sample holder out of the column 2. Adjust the illumination on the screen 3. Insert camera 4. Adjust beam intensity to ~50% 5. Take an image. Now measure the histogram of "Gray value distribution" in the recorded image (Main manu: Measure -} Histogram). Look at the course of the histogram line. If it is smooth and the distribution is narrow (~100 for MegaVieew II is OK) then you do not need to adjust "shading correction". Otherwise you should take a new set of gain and bias images.
My best regards
Oldrich
-- Old=oich Benada Institute of Microbiology CAS, v.v.i. Laboratory of Molecular Structure Characterization V/ {} de=ask/* 1083 142 20 Prague 4 Czech Republic
On Thu, 18 Feb 2016 04:19:53 -0600, nizets2-at-yahoo.com wrote : } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Listers, } } we have a SIS Megaview III camera (SIS, then Olympus and now I see it } is EMSIS "again") for over 10 years, a real workhorse, always } performing well without complaining. Because it works so well I tend } to forget about it and now I am just wondering if I need to update } the gain and bias corrections (the correction pictures)? I can't } remember the last time I did it but it is a long time ago. I know } that it is not a lot of work to do it, I just wondered if there is } any reason to do this regularly and if yes, what are the time } intervals. Many thanks in advance. } } Regards } Stephane } } ==============================Original } Headers============================== 3, 29 -- From nizets2-at-yahoo.com } Thu Feb 18 04:08:18 2016 3, 29 -- Received: from } nm14-vm0.bullet.mail.bf1.yahoo.com } (nm14-vm0.bullet.mail.bf1.yahoo.com [98.139.213.164]) 3, 29 -- } by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } u1IA8HGp022220 3, 29 -- for {microscopy-at-microscopy.com} ; Thu, } 18 Feb 2016 04:08:17 -0600 3, 29 -- DKIM-Signature: v=1; } a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1455790083; } bh=x7ccEl93SQjy6RmTa7YGFTI9AoSGhaKtVxThqGCF8uI=; } h=Date:From:Reply-To:To:Subject:References:From:Subject; } b=mxmCwIJ35ltTrPD3kub/zVxqaafRcOAxfgm6ezzoJrp1pUCYmcfPSQRcz2UZCYB7E2VDYutG4Acwoc6fb+dgYjGb/qn0SYRRjmHssI4OgoU5KzEsVT7z/hXuvkawJQrdxcMBZFjaSBsgwQW/NqldgKsdDF7QxPC3nogGqNgnXcC8R6tuyu5iftY8Mv4hEEhxZBqjkVbYVTJD4II/3DuBYpGu2M8zgAudq87whg7+Fz8BK1DwDQbxqX6RVf7LeWJ6wCuaLj+MlbtnyWJgVCLjFByywNR82D973IPAXq8MANbIjz9vlXDO0aK/VOoD3HTLmooRkrHVEPifDqHg+3oWrw== } 3, 29 -- Received: from [98.139.215.143] by } nm14.bullet.mail.bf1.yahoo.com with NNFMP; 18 Feb 2016 10:08:03 -0000 } 3, 29 -- Received: from [98.139.212.220] by } tm14.bullet.mail.bf1.yahoo.com with NNFMP; 18 Feb 2016 10:08:03 -0000 } 3, 29 -- Received: from [127.0.0.1] by omp1029.mail.bf1.yahoo.com } with NNFMP; 18 Feb 2016 10:08:03 -0000 3, 29 -- } X-Yahoo-Newman-Property: ymail-3 3, 29 -- X-Yahoo-Newman-Id: } 741770.20773.bm-at-omp1029.mail.bf1.yahoo.com 3, 29 -- X-YMail-OSG: } 9nq693UVM1nU.BaDUcqJJgdhtWG7s9wmJYCi2MeGsNslachC50ErOqvae9Gx8Ka 3, 29 } -- } 9zbrt5rq.gYAwbe3oLBiRmOMyG.BYkEej7Am1ukFXfhrPI_nmsIA4VEGAF51eLmld4zOtlbFAkFv } 3, 29 -- } U0L7c8ugwikXIHKGOHBVEauDmO7yx579GW9GdgZMgsvNawS1.8XXpys4zbpaG3N0hvBLDNqQ8cyj } 3, 29 -- } 1TEsA7jPiu6pxVkQ0AaY.w_9g8PB9XnyXBH.mz3Jp5TR9rQoxU7sHPwPjy_JqJd3m83fyNFhvPuQ } 3, 29 -- } m3dVQ1abp.DifVvCA3Yb4FRYqIDZCOPX0xg8XKxpyzpYnmBp69hrSrCfj_6Snc8sD6UU9NOxJ93f } 3, 29 -- } dJeWO1GznuG5eY4hKCAH0vmlMa8agqeM.M4tSZaIXP09yXCgaEMSAj.aDIpN4kUvtoJYN9MczS3D } 3, 29 -- } GJoW6ohYzYMk05lp.BH7g8D8g_6wqQxWiS08xJAMwxRaDjlVrDb41LV9KtUsHqodh4TQT3Z2DqFT } 3, 29 -- XNnKGXA-- 3, 29 -- Received: by 66.196.80.114; Thu, 18 Feb } 2016 10:08:03 +0000 3, 29 -- Date: Thu, 18 Feb 2016 10:08:02 +0000 } (UTC) 3, 29 -- From: Stephane Nizet {nizets2-at-yahoo.com} 3, 29 -- } Reply-To: Stephane Nizet {nizets2-at-yahoo.com} 3, 29 -- To: } {microscopy-at-microscopy.com} 3, 29 -- Message-ID: } {198161144.6166998.1455790082942.JavaMail.yahoo-at-mail.yahoo.com} 3, 29 } -- Subject: bias and gain correction on TEM cameras 3, 29 -- } MIME-Version: 1.0 3, 29 -- Content-Type: text/plain; charset=UTF-8 3, } 29 -- Content-Transfer-Encoding: 7bit 3, 29 -- References: } {198161144.6166998.1455790082942.JavaMail.yahoo.ref-at-mail.yahoo.com} } ==============================End of - } Headers==============================
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==============================Original Headers============================== 12, 48 -- From benada-at-biomed.cas.cz Thu Feb 18 05:39:25 2016 12, 48 -- Received: from barracuda.biomed.cas.cz (barracuda.biomed.cas.cz [147.231.40.11]) 12, 48 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1IBdOm4001310 12, 48 -- for {microscopy-at-microscopy.com} ; Thu, 18 Feb 2016 05:39:25 -0600 12, 48 -- X-ASG-Debug-ID: 1455795548-05011e54257f2db0001-4CH8be 12, 48 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) by barracuda.biomed.cas.cz with ESMTP id tXxpXOM8wjJ82mks (version=TLSv1 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); Thu, 18 Feb 2016 12:39:08 +0100 (CET) 12, 48 -- X-Barracuda-Envelope-From: benada-at-biomed.cas.cz 12, 48 -- X-Barracuda-Effective-Source-IP: mail2.biomed.cas.cz[147.231.40.32] 12, 48 -- X-Barracuda-Apparent-Source-IP: 147.231.40.32 12, 48 -- Received: from u117ob02 (c111jn.mbu.cas.cz [147.231.44.12]) 12, 48 -- (using TLSv1 with cipher AES128-SHA (128/128 bits)) 12, 48 -- (No client certificate requested) 12, 48 -- by mail2.biomed.cas.cz (Postfix) with ESMTPSA id DB7002C80E8; 12, 48 -- Thu, 18 Feb 2016 12:39:04 +0100 (CET) 12, 48 -- Date: Thu, 18 Feb 2016 12:39:04 +0100 12, 48 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 12, 48 -- To: nizets2-at-yahoo.com 12, 48 -- Cc: {microscopy-at-microscopy.com} 12, 48 -- Subject: Re: [Microscopy] bias and gain correction on TEM cameras 12, 48 -- Message-ID: {20160218123904.70cccbb3-at-u117ob02} 12, 48 -- X-ASG-Orig-Subj: Re: [Microscopy] bias and gain correction on TEM cameras 12, 48 -- In-Reply-To: {201602181019.u1IAJrKi025081-at-ns.microscopy.com} 12, 48 -- References: {201602181019.u1IAJrKi025081-at-ns.microscopy.com} 12, 48 -- Organization: =?UTF-8?B?TWlrcm9iaW9sb2dpY2vDvSDDunN0YXY=?= AV 12, 48 -- =?UTF-8?B?xIxS?= 12, 48 -- X-Mailer: Claws Mail 3.11.1 (GTK+ 2.24.25; i586-pc-linux-gnu) 12, 48 -- MIME-Version: 1.0 12, 48 -- Content-Type: text/plain; charset=UTF-8 12, 48 -- X-IoP-CAS-MailScanner-ID: DB7002C80E8.86B0F 12, 48 -- X-IoP-CAS-MailScanner: Processed 12, 48 -- X-Spam-Status: No 12, 48 -- X-Barracuda-Connect: mail2.biomed.cas.cz[147.231.40.32] 12, 48 -- X-Barracuda-Start-Time: 1455795548 12, 48 -- X-Barracuda-Encrypted: DHE-RSA-AES256-SHA 12, 48 -- X-Barracuda-URL: https://barracuda.biomed.cas.cz:443/cgi-mod/mark.cgi 12, 48 -- X-Barracuda-Scan-Msg-Size: 6193 12, 48 -- X-Virus-Scanned: by bsmtpd at biomed.cas.cz 12, 48 -- X-Barracuda-BRTS-Status: 1 12, 48 -- X-Barracuda-Spam-Score: 0.50 12, 48 -- X-Barracuda-Spam-Status: No, SCORE=0.50 using per-user scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=9.0 tests=BSF_RULE7568M, BSF_SC0_MISMATCH_TO 12, 48 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.27131 12, 48 -- Rule breakdown below 12, 48 -- pts rule name description 12, 48 -- ---- ---------------------- -------------------------------------------------- 12, 48 -- 0.00 BSF_SC0_MISMATCH_TO Envelope rcpt doesn't match header 12, 48 -- 0.50 BSF_RULE7568M Custom Rule 7568M 12, 48 -- Content-Transfer-Encoding: 8bit 12, 48 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u1IBdOm4001310 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both 12hy1-at-queensu.ca as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: 12hy1-at-queensu.ca Name: Hongbing Yu
Organization: Queen's University at Kingston
Title-Subject: [Filtered] How batch convert emi to other format
Message: Dear all, We have an FEI TEM microscope. The STEM images Acquired in TIA software are saved in emi format. I can open them in TIA software, and can only export them one by one. That is quite annoying. Is there anybody who has any idea how to batch convert the images?
Thanks Hongbing Yu
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Email: mlibbee-at-lbl.gov Name: Marissa Libbee
Organization: LBL
Title-Subject: [Filtered] Attn: LaB6 cathode/e-gun mfrs and vendors
Message: I'm interested in tracking down mfrs and vendors of anything LaB6 related. Please contact me offline with company names and preferably, a contact name within the company. Thanks!
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Title-Subject: [Filtered] EM Connectome Annotation Team Manager (362-908 )
Message: The Janelia Research Campus of the Howard Hughes Medical Institute (Janelia) is using high-throughput electron microscopy (EM) to completely image multiple brains and nervous systems of the fruit fly, Drosophila melanogaster. Janelia scientists will utilize these massive data sets to reconstruct neuronal circuits, ultimately culminating in the complete connectome-oor wiring diagram-oof the fly brain. The connectome will provide the most complete anatomical description of a complex nervous system to date in an organism uniquely suited to combine this anatomical information with functional imaging, electrophysiology, quantitative behavioral assays, precise genetic manipulation of identified cell types and computational modeling. Thus we expect these circuit-level reconstructions to inform specific biological experiments, enabling dramatic leaps in our understanding of brain function.
Janelia is seeking a talented individual to assemble, train, and lead a group of approximately ten neuron tracing staff, with potential for a significantly larger effort based on the established success of the initial team. In order to be successful, the manager must have research experience in neuroanatomy, preferably in the application of electron microscopy to elucidate neurobiological function. Knowledge of Drosophila biology and/or software for reconstructing objects from serial section images is strongly desired. The manager will collaborate with scientists (at Janelia and elsewhere) whose research is targeted toward understanding of specific circuits and functions in the fly brain, guiding their team of highly trained specialists to identify, trace, and annotate specific collections of neurons from the vast data being produced. The manager will also collaborate closely with the project teams producing the EM data and developing the algorithms and tools that enable the work of their team. Thus, the ideal candidate for this position must have a neuroscience background, the ability to teach scientific concepts, and experience in supervising and leading the work of others in a dynamic setting. The candidate must further possess an innate sense of pace and urgency, and excel at working in a team science environment to achieve challenging goals, prioritizing multiple assignments, and communicating clearly to a broad group of collaborators. Experience in supervising and leading the work of others is desired but not strictly required.
HHMI is an Equal Opportunity Employer.
Please apply online at https://hhmi-openhire.silkroad.com/epostings/index.cfm?fuseaction=app.jobinfo&jobid=362&company_id=16908&version=1&source=ONLINE&jobOwner=992274&aid=1.
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TIA folder export: - Open menu Tools\Components. - Click ,AeuInstall,Aeu. - Type ,Aeufolderexport.wsc,Aeu. - ,Aeuok". Now that this component is installed, there will be an additional menu in the processing tasks. Check settings and hit Export.
Best,
Wim Hagen EMBL Heidelberg
} On Feb 19, 2016, at 1:58 AM, microscopy.listserver-at-gmail.com wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: 12hy1-at-queensu.ca } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both 12hy1-at-queensu.ca as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: 12hy1-at-queensu.ca } Name: Hongbing Yu } } Organization: Queen's University at Kingston } } Title-Subject: [Filtered] How batch convert emi to other format } } Message: Dear all, } We have an FEI TEM microscope. The STEM images Acquired in TIA software are saved in emi format. I } can open them in TIA software, and can only export them one by one. That is quite annoying. Is there } anybody who has any idea how to batch convert the images? } } Thanks } Hongbing Yu } } } Login Host: 130.15.34.113 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } } -- } =========================================== } Do not reply to this message it is from } the Microscopy Listserver NO-REPLY forwarding } system. 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==============================Original Headers============================== 7, 27 -- From wim.hagen-at-me.com Thu Feb 18 20:53:36 2016 7, 27 -- Received: from pv33p04im-asmtp001.me.com (pv33p04im-asmtp001.me.com [17.143.181.10]) 7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1J2raff023321 7, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 18 Feb 2016 20:53:36 -0600 7, 27 -- Received: from wim-hagens-macbook-pro.local.pool.embl.de 7, 27 -- (unknown [194.94.44.220]) by pv33p04im-asmtp001.me.com 7, 27 -- (Oracle Communications Messaging Server 7.0.5.36.0 64bit (built Sep 8 2015)) 7, 27 -- with ESMTPSA id {0O2R0085PXCMH640-at-pv33p04im-asmtp001.me.com} for 7, 27 -- Microscopy-at-microscopy.com; Fri, 19 Feb 2016 02:53:14 +0000 (GMT) 7, 27 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:,, 7, 27 -- definitions=2016-02-19_02:,, signatures=0 7, 27 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 7, 27 -- clxscore=1011 suspectscore=2 malwarescore=0 phishscore=0 adultscore=0 7, 27 -- bulkscore=0 classifier=spam adjust=0 reason=mlx scancount=1 7, 27 -- engine=8.0.1-1510270003 definitions=main-1602190039 7, 27 -- Content-type: text/plain; charset=utf-8 7, 27 -- MIME-version: 1.0 (Mac OS X Mail 8.2 \(2104\)) 7, 27 -- Subject: Re: [Microscopy] viaWWW:How batch convert emi to other format 7, 27 -- From: Wim Hagen {wim.hagen-at-me.com} 7, 27 -- In-reply-to: {201602190058.u1J0wuXt027832-at-ns.microscopy.com} 7, 27 -- Date: Fri, 19 Feb 2016 03:53:09 +0100 7, 27 -- Message-id: {EA4C4200-6FBC-4DD6-9DC2-B4F92F0BF494-at-me.com} 7, 27 -- References: {201602190058.u1J0wuXt027832-at-ns.microscopy.com} 7, 27 -- To: 12hy1-at-queensu.ca, Microscopy-at-microscopy.com 7, 27 -- X-Mailer: Apple Mail (2.2104) 7, 27 -- Content-Transfer-Encoding: 8bit 7, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u1J2raff023321 ==============================End of - Headers==============================
I am need of some experience here. Has anyone played with UA replacements such samarium and gadolinium triacetate for en bloc staining? How does this compare in performance, permeation, precipitation etc? Is it an acceptable alternative for all tissues or just selected ones. I am aware of many papers on the mater, and my own dabbling but I tend to find the microscopy list opinion a little easier to swallow.
Thanks in advance J
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After decades of using standard organic solvents for paraffin- section histology, I find that I've become highly allergic to the fumes.-* These include commercial preps like Citrosolv and Hemo-De, as well as xylene and toluene, which cause dizziness and pounding headaches.-* This has made staining and coverslipping very difficult, even with-* hood.-*-*-*Can anyone recommend alternative solutions for (a) dewaxing and (b) coverslipping with Permount?-* thanks very much!
Daniel Blackburn Biology, Trinity College (Hartford CT) -*
==============================Original Headers============================== 4, 29 -- From dblackburn2000-at-yahoo.com Sat Feb 20 10:55:19 2016 4, 29 -- Received: from nm13-vm0.bullet.mail.bf1.yahoo.com (nm13-vm0.bullet.mail.bf1.yahoo.com [98.139.213.79]) 4, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1KGtJqN017594 4, 29 -- for {Microscopy-at-microscopy.com} ; Sat, 20 Feb 2016 10:55:19 -0600 4, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1455987305; bh=GYAjj76GzomM7vop6UUnJyXkz0u+wZUkwa3SDd0g15A=; h=Date:From:Reply-To:To:Subject:References:From:Subject; b=TPxqPNC/tvQBHsTKLGuQ51Jxg7PrRk1Z77IjDYAtNrZPBfyLnjxprl57gqSAmcE6keQ080eKlTjoR/clH40sa3IPkQqVdl3HUZaugXs9TzpNOF+PJyPx7Ey2G68vm51CkrE0pfnoYku9ZaNs+8dasZxwEoJOt5N4tyekwNeyVH4GCpXRv4EFgmxNTtJtzt5Z6fJ9cGdjkoEooZibBz86jBWN70VTdga00AoibIBFXh/0g4qvxNfJKsxYB/XhmCgB/ZG4fK8She7vD2xu2k/W1HqNDBSK77t/7eLBoeuKid0158KLd6UXCr9j7zSErsJ0xwuMBw9LmRrmi9iC10fEHg== 4, 29 -- Received: from [66.196.81.170] by nm13.bullet.mail.bf1.yahoo.com with NNFMP; 20 Feb 2016 16:55:05 -0000 4, 29 -- Received: from [98.139.212.222] by tm16.bullet.mail.bf1.yahoo.com with NNFMP; 20 Feb 2016 16:55:05 -0000 4, 29 -- Received: from [127.0.0.1] by omp1031.mail.bf1.yahoo.com with NNFMP; 20 Feb 2016 16:55:05 -0000 4, 29 -- X-Yahoo-Newman-Property: ymail-3 4, 29 -- X-Yahoo-Newman-Id: 532123.73121.bm-at-omp1031.mail.bf1.yahoo.com 4, 29 -- X-YMail-OSG: pQ6H50EVM1mOAEYBONpigG3IZYomqCLazwII_MkU0fjcMa3_mrHLGMVY9za3Cb5 4, 29 -- rtfU9TxN46HHIjZZmoXVODUSN2PFdj3kF9Ae_nSuAc71MZ2azIzwmB4U4FJaEynMUv_j1mX4wReD 4, 29 -- mREMmAAZnMyIZTZuaQekhHvng_BLwBMnJ5vL9WPtVQxYrY2f1mGvDO4yzQZ31iy_AKLJtY9aZ2hV 4, 29 -- CeSPnr1ZrqqTnIR1XTfmWUi4OObTKxkXdcRKYUc0J7cKTEjtonO5nAK5yoXFFcZrxWTjUHSaQl8q 4, 29 -- 5E2S3qBWMCAyAOkd8im.uNmteAlj7usrcZXcQvG4cRrGBi8zNdGCptRXt2.2knijRUVID_.fcHWl 4, 29 -- 4XVdFdmAJirUe0hNywHM6LYT87WdteIDhiNyWHloS5g3DIr8jFKjILiRsuMQqSAiR.ERWgXqlZvj 4, 29 -- rvi0eS7x7hsidXf0j7fHi6aGnkElT8D8cxLc0Ivs2n9rouVgLONbz 4, 29 -- Received: by 76.13.27.50; Sat, 20 Feb 2016 16:55:05 +0000 4, 29 -- Date: Sat, 20 Feb 2016 16:55:04 +0000 (UTC) 4, 29 -- From: daniel blackburn {dblackburn2000-at-yahoo.com} 4, 29 -- Reply-To: daniel blackburn {dblackburn2000-at-yahoo.com} 4, 29 -- To: {Microscopy-at-microscopy.com} 4, 29 -- Message-ID: {370209020.7409633.1455987304853.JavaMail.yahoo-at-mail.yahoo.com} 4, 29 -- Subject: question - Allergy to histological solvents? 4, 29 -- MIME-Version: 1.0 4, 29 -- Content-Type: text/plain; charset=UTF-8 4, 29 -- References: {370209020.7409633.1455987304853.JavaMail.yahoo.ref-at-mail.yahoo.com} 4, 29 -- Content-Transfer-Encoding: 8bit 4, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u1KGtJqN017594 ==============================End of - Headers==============================
I assume you're doing these steps in a fume hood. One thing we've found for people who are sensitive to solvent fumes are charcoal-lined dust masks. Search amazon.com for "charcoal filter masks". We use the 3M type (but NOT for the prices here - we got ours through Fisher Scientific). So far, they've worked fine.
Looks like you've already tried the various xylene replacements.
Phil
Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
________________________________________ X-from: dblackburn2000-at-yahoo.com [dblackburn2000-at-yahoo.com] Sent: Saturday, February 20, 2016 12:16 PM To: Oshel, Philip Eugene
Hello
After decades of using standard organic solvents for paraffin- section histology, I find that I've become highly allergic to the fumes. These include commercial preps like Citrosolv and Hemo-De, as well as xylene and toluene, which cause dizziness and pounding headaches. This has made staining and coverslipping very difficult, even with hood. Can anyone recommend alternative solutions for (a) dewaxing and (b) coverslipping with Permount? thanks very much!
Daniel Blackburn Biology, Trinity College (Hartford CT)
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==============================Original Headers============================== 14, 35 -- From oshel1pe-at-cmich.edu Sun Feb 21 09:20:13 2016 14, 35 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116]) 14, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1LFKCwi014223 14, 35 -- for {microscopy-at-microscopy.com} ; Sun, 21 Feb 2016 09:20:13 -0600 14, 35 -- Received: from cas.cmich.edu (async2.cmich.edu [141.209.15.141] (may be forged)) 14, 35 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id u1LFJvtI005485; 14, 35 -- Sun, 21 Feb 2016 10:19:57 -0500 14, 35 -- Received: from cmail106.central.cmich.local ([fe80::a8:3e31:c722:be0a]) by 14, 35 -- cas2.central.cmich.local ([fe80::c44b:39ae:767:cab7%10]) with mapi id 14, 35 -- 14.03.0248.002; Sun, 21 Feb 2016 10:19:57 -0500 14, 35 -- From: "Oshel, Philip Eugene" {oshel1pe-at-cmich.edu} 14, 35 -- To: "dblackburn2000-at-yahoo.com" {dblackburn2000-at-yahoo.com} 14, 35 -- CC: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 14, 35 -- Subject: RE: [Microscopy] question - Allergy to histological solvents? 14, 35 -- Thread-Topic: [Microscopy] question - Allergy to histological solvents? 14, 35 -- Thread-Index: AQHRbAJzvIVVjB+UVkiX60gI5zJcWJ82nTv9 14, 35 -- Date: Sun, 21 Feb 2016 15:19:57 +0000 14, 35 -- Message-ID: {FB5ED57CF3415D4F8C5F93F1DE5468732FCE720F-at-cmail106.central.cmich.local} 14, 35 -- References: {201602201716.u1KHGmXC002359-at-ns.microscopy.com} 14, 35 -- In-Reply-To: {201602201716.u1KHGmXC002359-at-ns.microscopy.com} 14, 35 -- Accept-Language: en-US 14, 35 -- Content-Language: en-US 14, 35 -- X-MS-Has-Attach: 14, 35 -- X-MS-TNEF-Correlator: 14, 35 -- x-originating-ip: [141.209.2.100] 14, 35 -- Content-Type: text/plain; charset="us-ascii" 14, 35 -- MIME-Version: 1.0 14, 35 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 14, 35 -- X-Spam-Score: -6.00 () [Hold at 6.00] L_CTCMUM,L_EXCH_MF,RDNS_NONE,_L_LEXNRL,_L_LLEXCH,SPF(softfail:0),RBL(rp-good:-0.1),Bayes(0.0001:-0.5) 14, 35 -- X-CanIt-Geo: ip=141.209.15.141; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6 14, 35 -- X-CanItPRO-Stream: default 14, 35 -- X-Canit-Stats-ID: 05Ql3jVMv - 2337180cc265 - 20160221 14, 35 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.15.116 14, 35 -- Content-Transfer-Encoding: 8bit 14, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u1LFKCwi014223 ==============================End of - Headers==============================
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A serious problem. By listing, I have come with suggestions. I have long used a Gum Damar formulation that I cannot offer, because I have prepared it in xylene.
NOTE: for those who might be interested - or users of Damar - I have a liter that I will NEVER use more than a few ml's over my reasonably extended age - now 76. Further, I don't do paraffin any more.
The suggestions:
If you can arrive at a solvent for Damar that is miscible with xylene/toluene, then it would be possible that you could prepare your own Damar. NOTE: Damar applied in the 1960's still have no sign of drying/cracking/crystalizing. Source: http://www.eco-house.com/shop/950-damar-resin-crystals-graded/
You might test a hydrophilic mountant. Source: https://www.emsdiasum.com/microscopy/products/histology/mounting_media.aspx
If your allergy is broad-band, then you may have to resort to the latter. If it is more specific - just for those you mentioned - then your options might include Canada Balsam that is very natural, but also often found with benzene or xylene or toulene. I have some that will never be used as it requires some solvent that might not raise a sneeze.
Email me directly if you wish to discuss further,
Frederick C Monson, PhD Center for Microanalysis and Imaging, Research and Training (CMIRT) West Chester University of Pannsylvania West Chester, PA, 1938 610-738-0437 fmonson-at-wcupa.edu
________________________________________ X-from: daniel blackburn via Histonet {histonet-at-lists.utsouthwestern.edu} Sent: Saturday, February 20, 2016 2:44 PM To: Histonet-at-lists.utsouthwestern.edu
X-from: mp76ers-at-gmail.com
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Email: mp76ers-at-gmail.com Name: Mojahed
Organization: MERC
Title-Subject: [Filtered] Cambridge S360 SEM Computer Error
Message: Hi to all, I have a problem with the microcomputer (SBC) of an old s360 SEM. It's model is PME 68-2 (or PME 68-12). It has s1-s8 LEDs on its front panel for error checking. Now s5 LED indicate en error. Does anyone have its manual or know what does it mean?
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I would like to hear from anyone out there who is using FEI's TeneoVS SEM. I want to know your experiences with it and I would like to get a sample run as a demo, we can cover any associated costs.
Tom Bargar UNMC Electron Microscopy Core Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
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==============================Original Headers============================== 7, 37 -- From tbargar-at-unmc.edu Mon Feb 22 08:30:10 2016 7, 37 -- Received: from zixvpm01.unmc.edu (zixvpm01.unmc.edu [192.198.54.126]) 7, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1MEUA1r001242 7, 37 -- for {Microscopy-at-microscopy.com} ; Mon, 22 Feb 2016 08:30:10 -0600 7, 37 -- Received: from 127.0.0.1 (ZixVPM [127.0.0.1]) 7, 37 -- by Outbound.unmc.edu (Proprietary) with SMTP id EAA6F5C5E75 7, 37 -- for {Microscopy-at-microscopy.com} ; Mon, 22 Feb 2016 08:07:31 -0600 (CST) 7, 37 -- Received: from UNMCEX1.unmcresforest.org (unknown [10.8.3.1]) 7, 37 -- (using TLSv1 with cipher AES128-SHA (128/128 bits)) 7, 37 -- (No client certificate requested) 7, 37 -- by zixvpm01.unmc.edu (Proprietary) with ESMTPS id 02E195C5E6A 7, 37 -- for {Microscopy-at-microscopy.com} ; Mon, 22 Feb 2016 08:07:31 -0600 (CST) 7, 37 -- Received: from UNMCEX2.unmcresforest.org ([fe80::151d:89d:49b6:a0ab]) by 7, 37 -- UNMCEX1.unmcresforest.org ([fe80::2023:ee90:3fae:f0d6%12]) with mapi id 7, 37 -- 14.03.0235.001; Mon, 22 Feb 2016 08:29:55 -0600 7, 37 -- From: "Bargar, Tom W" {tbargar-at-unmc.edu} 7, 37 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 7, 37 -- Subject: Users of FEI TeneoVS SEM 7, 37 -- Thread-Topic: Users of FEI TeneoVS SEM 7, 37 -- Thread-Index: AdFtfSnkLKYP9x9wRmufl/4nk4jZUQ== 7, 37 -- Date: Mon, 22 Feb 2016 14:29:53 +0000 7, 37 -- Message-ID: {E5858BD0583CA8499131AC34AD6766B0AE854050-at-UNMCEX2.unmcresforest.org} 7, 37 -- Accept-Language: en-US 7, 37 -- Content-Language: en-US 7, 37 -- X-MS-Has-Attach: 7, 37 -- X-MS-TNEF-Correlator: 7, 37 -- x-originating-ip: [10.8.64.15] 7, 37 -- Content-Type: text/plain; charset="us-ascii" 7, 37 -- MIME-Version: 1.0 7, 37 -- X-VPM-MSG-ID: 22c5e17d-fb11-48c5-a02c-43161552b8cd 7, 37 -- X-VPM-HOST: zixvpm01.unmc.edu 7, 37 -- X-VPM-GROUP-ID: 668ad697-ab40-4dca-8a37-267e6a56aa92 7, 37 -- X-VPM-ENC-REGIME: ZixVPM,Plaintext 7, 37 -- X-VPM-CERT-FLAG: 0 7, 37 -- X-VPM-IS-HYBRID: 0 7, 37 -- Content-Transfer-Encoding: 8bit 7, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u1MEUA1r001242 ==============================End of - Headers==============================
Unfortunately, you,Aeove probably lost your sense of smell and taste if you,Aeove become that sensitized. I would suggest the quickest remedy may be using a half-mask respirator with organic vapor filter cartridges, even though you may be using a fume hood. I think paint respirators may work since they filter organics/solvents/paints. Make sure it fits correctly too. It won't do to have a proper filtering assembly if it doesn't fit correctly! Good luck. ~Winston
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Winston W. Wiggins, E.M. Pathology Specialist CEDARS-SINAI MEDICAL CENTER, Pathology & Lab Medicine Electron Microscopy, LLSPT, A828 8700 Beverly Blvd. Los Angeles, CA 90048-1865 OAess310-423-1363 (off); 310-423-5323 (lab); 310-423-0685 (fax) Winston.Wiggins-at-CSHS.org ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
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==============================Original Headers============================== 5, 50 -- From winston.wiggins-at-cshs.org Mon Feb 22 10:09:23 2016 5, 50 -- Received: from espxlsmg04.csmc.edu (mx4.csmc.edu [192.231.86.201]) 5, 50 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1MG9Mof024718 5, 50 -- for {Microscopy-at-microscopy.com} ; Mon, 22 Feb 2016 10:09:23 -0600 5, 50 -- X-AuditID: 0a200141-f790b6d00000272b-9e-56cb32a01a48 5, 50 -- Received: from CSHSMSGZIX04.CSMC.EDU (cshsmsgzix04.csmc.edu [10.17.158.163]) 5, 50 -- by espxlsmg04.csmc.edu (CS) with SMTP id 7C.A0.10027.0A23BC65; Mon, 22 Feb 2016 08:09:04 -0800 (PST) 5, 50 -- Received: from 127.0.0.1 (ZixVPM [127.0.0.1]) 5, 50 -- by Outbound.cshs.org (Proprietary) with SMTP id 0B0DC7203FD 5, 50 -- for {Microscopy-at-microscopy.com} ; Mon, 22 Feb 2016 08:04:56 -0800 (PST) 5, 50 -- Received: from ESPWMSGHUB05.CSMC.EDU (espwmsghub05.csmc.edu [10.17.239.90]) 5, 50 -- by CSHSMSGZIX04.CSMC.EDU (Proprietary) with ESMTP id D42CD7203FB 5, 50 -- for {Microscopy-at-microscopy.com} ; Mon, 22 Feb 2016 08:04:55 -0800 (PST) 5, 50 -- Received: from CSHSMSGMBX01.CSMC.EDU ([169.254.3.125]) by 5, 50 -- ESPWMSGHUB05.CSMC.EDU ([10.17.239.90]) with mapi id 14.03.0235.001; Mon, 22 5, 50 -- Feb 2016 08:09:08 -0800 5, 50 -- From: "Wiggins, Winston" {Winston.Wiggins-at-cshs.org} 5, 50 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 5, 50 -- Subject: RE: question - Allergy to histological solvents? 5, 50 -- Thread-Topic: RE: question - Allergy to histological solvents? 5, 50 -- Thread-Index: AdFtiEeekRFJ5XMDSxmYMvDNpL2XNw== 5, 50 -- Date: Mon, 22 Feb 2016 16:09:07 +0000 5, 50 -- Message-ID: {77186365EA63114782772AB6D4940EEE7A76049A-at-cshsmsgmbx01.CSMC.EDU} 5, 50 -- Accept-Language: en-US 5, 50 -- Content-Language: en-US 5, 50 -- X-MS-Has-Attach: 5, 50 -- X-MS-TNEF-Correlator: 5, 50 -- x-originating-ip: [166.124.28.190] 5, 50 -- Content-Type: text/plain; charset="utf-8" 5, 50 -- MIME-Version: 1.0 5, 50 -- X-VPM-MSG-ID: ecee1c8c-3893-4e58-a917-854ca910f97f 5, 50 -- X-VPM-HOST: CSHSMSGZIX04.CSMC.EDU 5, 50 -- X-VPM-GROUP-ID: f17f7004-8acd-4c98-922c-6736a276b9ca 5, 50 -- X-VPM-ENC-REGIME: Plaintext 5, 50 -- X-VPM-CERT-FLAG: 0 5, 50 -- X-VPM-IS-HYBRID: 0 5, 50 -- X-Brightmail-Tracker: H4sIAAAAAAAAA+NgFtrKIsWRmVeSWpSXmKPExsXCJThvse4Co9NhBu+mC1j8OdzA7sDo8bH7 5, 50 -- NVMAYxSXTUpqTmZZapG+XQJXxr5nU9gKNvFVfJvv18DYwNfFyMEhIWAi8XRSaBcjJ5ApJnHh 5, 50 -- 3nq2LkYuDiGBzYwSKw//ZoRwtjBKnHm2HMq5zijRe2obE4SzkFHiRd9qJpBRbALGEu/W5IKM 5, 50 -- EhGwlfh24Q4TiC0sYCkx/+kmRoi4ncS5viUsELaexKbJp8DiLAKqEidfHmEFsXkFfCT6bnYx 5, 50 -- g9iMQCd9P7UGbA6zgLjErSfzmSBOFZBYsuc8M4QtKvHy8T9WCFtJYt36i2wg5zALaEqs36UP 5, 50 -- 0aooMaX7ITvEeEGJkzOfsECUG0nseHmRHcJWlNhz7h8bhG0i8X7jSai4tMSqF3NZIIElJHH7 5, 50 -- lBZEWEji7Il7rBMYpWchOW4WwuJZSBbPQrJ4ASPLKkbh1OKCipzi3HQDE73k4txkvdSU0k2M 5, 50 -- oMhUYHTcwTjhku0hRgEORiUeXgPOU2FCrIllxZW5hxglOJiVRHjrpE6HCfGmJFZWpRblxxeV 5, 50 -- 5qQWH2KU5mBREuft3nw8TEggPbEkNTs1tSC1CCbLxMEp1cDYXx7+Vfbnh9k++qw24QziSvn/ 5, 50 -- Ju3defxFk9rOP50n6wqV5kvVeUZYF5VccLZY7nGmZdIWrmVRWc6xpSJMl19tXiKu/N09JDrx 5, 50 -- +j8RqwAvo3tznX7xC3zQ4pQ39Xgj+f3C9nY3uWnnSlWXK6gdkVdhtPW68HtNk1XohFU5vRNk 5, 50 -- KjS3TY1/+VmJpTgj0VCLuag4EQAiRscByAIAAA== 5, 50 -- Content-Transfer-Encoding: 8bit 5, 50 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id u1MG9Mof024718 ==============================End of - Headers==============================
Mississippi State University's College of Engineering will be hosting a Research Experience for Undergraduates May 31-Aug. 6.
Research will be in Materials Science and Engineering and will include topics such as biomaterials, nanoparticles, composites & polymers, and nanocrystalline materials. The program will also include professional and development seminars, industry site visits, and GRE preparation workshops.
The REU includes a weekly stipend, on-campus housing, and a meal allowance. For more information and/or to apply on-line please visit the college of engineering website: www.bagley.msstate.edu/REU/.
Amanda Lawrence Outreach Coordinator/Research Associate Institute for Imaging and Analytical Technologies Mississippi State University
==============================Original Headers============================== 7, 33 -- From ALawrence-at-i2at.msstate.edu Mon Feb 22 16:04:05 2016 7, 33 -- Received: from chokecherry.its.msstate.edu (chokecherry.its.msstate.edu [130.18.2.120]) 7, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1MM45OT025147 7, 33 -- for {Microscopy-at-microscopy.com} ; Mon, 22 Feb 2016 16:04:05 -0600 7, 33 -- Received: from mail06.ad.msstate.edu (mail06.ad.msstate.edu [130.18.230.65]) 7, 33 -- by chokecherry.its.msstate.edu (8.13.8/8.13.8) with ESMTP id u1MM3pgk011042 7, 33 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=FAIL) 7, 33 -- for {Microscopy-at-microscopy.com} ; Mon, 22 Feb 2016 16:03:51 -0600 7, 33 -- X-Sender: {} 7, 33 -- Received: from MAIL02.ad.msstate.edu (2002:8212:e63d::8212:e63d) by 7, 33 -- mail06.ad.msstate.edu (2002:8212:e641::8212:e641) with Microsoft SMTP Server 7, 33 -- (TLS) id 15.0.913.22; Mon, 22 Feb 2016 16:03:50 -0600 7, 33 -- Received: from MAIL02.ad.msstate.edu ([fe80::7846:3039:9492:24b0]) by 7, 33 -- mail02.ad.msstate.edu ([fe80::7846:3039:9492:24b0%13]) with mapi id 7, 33 -- 15.00.0913.011; Mon, 22 Feb 2016 16:03:50 -0600 7, 33 -- From: "Lawrence, Amanda" {ALawrence-at-i2at.msstate.edu} 7, 33 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 7, 33 -- Subject: summer research experience for engineering and materials science 7, 33 -- undergraduates 7, 33 -- Thread-Topic: summer research experience for engineering and materials 7, 33 -- science undergraduates 7, 33 -- Thread-Index: AdFtvC1M/yUDJOBSSVSKwPnrpzqTTg== 7, 33 -- Date: Mon, 22 Feb 2016 22:03:50 +0000 7, 33 -- Message-ID: {485c84d1773740c2856dbc8514520eb2-at-mail02.ad.msstate.edu} 7, 33 -- Accept-Language: en-US 7, 33 -- Content-Language: en-US 7, 33 -- X-MS-Has-Attach: 7, 33 -- X-MS-TNEF-Correlator: 7, 33 -- x-originating-ip: [130.18.230.93] 7, 33 -- Content-Type: text/plain; charset="us-ascii" 7, 33 -- MIME-Version: 1.0 7, 33 -- Content-Transfer-Encoding: 8bit 7, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u1MM45OT025147 ==============================End of - Headers==============================
From advertise.bz222dcedu-at-gmail.com Wed Feb 24 00:57:18 2016 Return-Path: {advertise.bz222dcedu-at-gmail.com} Received: from gmail.com ([121.78.112.171]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id u1O6vFHM030289 for {microscopylistserverarchive5-at-microscopy.com} ; Wed, 24 Feb 2016 00:57:16 -0600 Message-ID: {2EC237BE.15C3727C-at-gmail.com}
In 1921, Ms.Valencia Arton took for her second term, summer quarter a course called Textiles at the University of Chicago. She constructed a lab book consisting of experiments, typed and long hand notes and samples of fabric before and after testing.
I've found her notebook over 30 years ago in a used bookstore thinking, "What a source of old fabric samples and fibers" but I never did anything with it.
I'm sure Ms. Arton is long past caring, but I can't help but think some microscopist could find a publication in these pages, if they look hard enough. Valencia would probably enjoy that.
The book is free to anyone who would want it, no strings attached. She does mention the microscope and it is an interesting look at the analytical fiber and fabric procedures of 1921.
Contact me if you have question or need more detail.........
Frank ARDL
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==============================Original Headers============================== 10, 27 -- From frank_karl-at-ardl.com Wed Feb 24 07:07:25 2016 10, 27 -- Received: from cal1-mh746b.smtproutes.com (cal1-mh746b.smtproutes.com [208.70.89.157]) 10, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1OD7OdY006599 10, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 24 Feb 2016 07:07:24 -0600 10, 27 -- X-Katharion-ID: 1456319214.50048.cal1-mh746 10, 27 -- Received: from mail.ardl.com ([98.100.51.26]) by 10, 27 -- cal1-mh746.smtproutes.com [(192.69.16.68)] with ESMTP via TCP 10, 27 -- (TLSv1/TLS_RSA_WITH_AES_128_CBC_SHA); 24 Feb 2016 13:06:54 +0000 10, 27 -- Received: from exchange2k7.ad.ardl.com ([fe80::596e:af10:621c:b9f8]) by 10, 27 -- exchange2k7.ad.ardl.com ([fe80::596e:af10:621c:b9f8%14]) with mapi; Wed, 24 10, 27 -- Feb 2016 08:06:54 -0500 10, 27 -- From: Frank Karl {frank_karl-at-ardl.com} 10, 27 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 10, 27 -- Date: Wed, 24 Feb 2016 08:06:53 -0500 10, 27 -- Subject: Fiber and Fabric 10, 27 -- Thread-Topic: Fiber and Fabric 10, 27 -- Thread-Index: AdFvBDt1tIQveEdOS+ORmMZWmyaU5A== 10, 27 -- Message-ID: {0445DA8FFC235F4CBDAF9BACCF79E12108C792C0-at-exchange2k7.ad.ardl.com} 10, 27 -- Accept-Language: en-US 10, 27 -- Content-Language: en-US 10, 27 -- X-MS-Has-Attach: 10, 27 -- X-MS-TNEF-Correlator: 10, 27 -- acceptlanguage: en-US 10, 27 -- Content-Type: text/plain; charset="us-ascii" 10, 27 -- MIME-Version: 1.0 10, 27 -- Content-Transfer-Encoding: 8bit 10, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u1OD7OdY006599 ==============================End of - Headers==============================
The Organizing Committee of Ultrapath XVIII (http://congress.ultrapathxviii.org) has extended the deadline for abstract submission to March 24, 2016. Your work is very important for the Society and your contribution to the Congress will help all of us to keep improving and spread the word about the continued importance of Ultrastructural Pathology.
With kind regards,
From the Organizing Committee,
A.P. Alves de Matos Chair of UltrapathXVIII
==============================Original Headers============================== 6, 46 -- From apamatos-at-gmail.com Wed Feb 24 08:08:11 2016 6, 46 -- Received: from mail-wm0-f52.google.com (mail-wm0-f52.google.com [74.125.82.52]) 6, 46 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1OE8BfF028573 6, 46 -- for {microscopy-at-microscopy.com} ; Wed, 24 Feb 2016 08:08:11 -0600 6, 46 -- Received: by mail-wm0-f52.google.com with SMTP id g62so6243159wme.0 6, 46 -- for {microscopy-at-microscopy.com} ; Wed, 24 Feb 2016 06:07:57 -0800 (PST) 6, 46 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 46 -- d=gmail.com; s=20120113; 6, 46 -- h=to:from:subject:message-id:date:user-agent:mime-version 6, 46 -- :content-transfer-encoding; 6, 46 -- bh=jGwYeYU2P9NVDSHXIC/ikbUi66LcT+w0CW3CNixU668=; 6, 46 -- b=AyWnZTkk64GX2E3ViGo4aeetpSqKRZBYdShbpzeUvbrrLEnLqFCZ8CyiDB2wmIZIzl 6, 46 -- 5VLSMTW2ztw8zgVsCZAjXDC+q2Uh6+j1qOseUMeO19zhZKAIWM19oqLR5Bnlvym07Fzs 6, 46 -- XUj4E46nj4nHpWiVWpVvmASogYRUGtLjyppPVZ1FufqulY9odLuIagYASTAwHDHuKHZd 6, 46 -- tglqfmWGzHUcB5Oqq6uB2GruiClY9DfkhG8tM43YJ0C9Zgi0L3nMpm0rLH7CGrATXndm 6, 46 -- qkld+/dCBHFU3J+paFA6OcZtAeVj80Ot+fPWqKF5dqGz/vBXIYG8q7y8NUmzbGCpCjB2 6, 46 -- LZoQ== 6, 46 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 46 -- d=1e100.net; s=20130820; 6, 46 -- h=x-gm-message-state:to:from:subject:message-id:date:user-agent 6, 46 -- :mime-version:content-transfer-encoding; 6, 46 -- bh=jGwYeYU2P9NVDSHXIC/ikbUi66LcT+w0CW3CNixU668=; 6, 46 -- b=RwpqrBKKMRzYx+dbOnsaxl+pkpC57UpteKuRJSo/ikl//4ZdxV9TWcdRPX5+qqitm+ 6, 46 -- In+biXygyuua0qWor3+IInJgcTobaGhjDVoE04iKr/cEtxxBbCO2ZGctbqM5f2KXwo1r 6, 46 -- digaDiPDAw9493jqxOMvXR6ZAIKSav5DVZZ+XH83+6RSejSeYkX0t38rH6VE3KhdN2sL 6, 46 -- hL/K5R1zCaY+/Et3oEBzUSYyIhf/reJXhz1az5E/4rj+C+IMrpCvuKTX30eOhnh2HNYA 6, 46 -- SGmX/ELYZp5NBY3cLqYNkAKNVCc/NTT/1C57e6UBmJEj8Wuyi6rIxP3a1KwubHcmEARJ 6, 46 -- 1K0Q== 6, 46 -- X-Gm-Message-State: AG10YOS50eWzhmbrpT34bvFzMofZ1s8ECgiP2LfRp851Ipps+eHzjsHHK2gBtLW91GMe3A== 6, 46 -- X-Received: by 10.28.14.140 with SMTP id 134mr23384508wmo.39.1456322876619; 6, 46 -- Wed, 24 Feb 2016 06:07:56 -0800 (PST) 6, 46 -- Received: from ?IPv6:2001:8a0:f946:ec01:4261:86ff:fe2b:8e36? ([2001:8a0:f946:ec01:4261:86ff:fe2b:8e36]) 6, 46 -- by smtp.gmail.com with ESMTPSA id gb9sm3204146wjb.26.2016.02.24.06.07.53 6, 46 -- for {microscopy-at-microscopy.com} 6, 46 -- (version=TLSv1/SSLv3 cipher=OTHER); 6, 46 -- Wed, 24 Feb 2016 06:07:54 -0800 (PST) 6, 46 -- To: microscopy-at-microscopy.com 6, 46 -- From: AP Alves de Matos {apamatos-at-gmail.com} 6, 46 -- Subject: Extension of the deadline for abstract submission to Ultrapath XVIII 6, 46 -- Message-ID: {56CDB939.6020308-at-gmail.com} 6, 46 -- Date: Wed, 24 Feb 2016 14:07:53 +0000 6, 46 -- User-Agent: Mozilla/5.0 (X11; Linux x86_64; rv:38.0) Gecko/20100101 6, 46 -- Thunderbird/38.6.0 6, 46 -- MIME-Version: 1.0 6, 46 -- Content-Type: text/plain; charset=utf-8; format=flowed 6, 46 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
An international microscopy conference. June 6 - 10 at McCrone Research Institute, Chicago
Join some of the world's leading amateur and professional microscopists for the 68th annual Inter/Micro conference, featuring: . Research presentations on techniques/instrumentation, industrial/environmental microscopy, and chemical/ forensic microscopy . Two-day workshop on microscopy of sand, taught by Thomas J. Hopen of the ATF . Evening with Brian Ford presentation: "Magical Mystery Tour of the Cell" . State Microscopical Society of Illinois Awards Dinner . ... and more! View the schedule of events.
Research presentations will be held June 6 - 8. Speakers will receive a $50 registration discount. Read abstract submission guidelines.
Use our secure online registration form at www.mcri.org to reserve your seat today.
We look forward to seeing you in Chicago!
McCrone Research Institute A Not-for-Profit Corporation 2820 South Michigan Avenue, Chicago, IL 60616-3230 Phone: 312-842-7100 Fax: 312-842-1078 www.mcri.org intermicro-at-mcri.org
......................................... Andrew Anthony "Tony" Havics, CIH, PE Environmental, Health & Safety, Microscopy, Materials Science & Forensic Engineering pH2, LLC 5250 E US Highway 36, Suite 830 Avon, IN 46123 (317) 718-7020 Office (317) 718-7038 Fax (317) 409-3238 Cell aahavics-at-pH2LLC.com www.ph2LLC.com
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==============================Original Headers============================== 13, 26 -- From ph2-at-sprynet.com Wed Feb 24 09:21:30 2016 13, 26 -- Received: from elasmtp-masked.atl.sa.earthlink.net (elasmtp-masked.atl.sa.earthlink.net [209.86.89.68]) 13, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1OFLUcR018529 13, 26 -- for {microscopy-at-microscopy.com} ; Wed, 24 Feb 2016 09:21:30 -0600 13, 26 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 13, 26 -- s=dk20050327; d=sprynet.com; 13, 26 -- b=Pt9RaMKcujcxSM58oP5jH3zXPJNwCIPl9wJnr5H2Xka2ymRIbFSVZGHyLwmaINyv; 13, 26 -- h=Received:From:To:Subject:Date:Message-ID:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Mailer:Thread-Index:Content-Language:X-ELNK-Trace:X-Originating-IP; 13, 26 -- Received: from [68.58.112.163] (helo=AAHHPdv7) 13, 26 -- by elasmtp-masked.atl.sa.earthlink.net with esmtpa (Exim 4.67) 13, 26 -- (envelope-from {ph2-at-sprynet.com} ) 13, 26 -- id 1aYbFR-0001Mw-68; Wed, 24 Feb 2016 10:21:05 -0500 13, 26 -- From: "Tony Havics, CHMM, CIH, PE" {ph2-at-sprynet.com} 13, 26 -- To: "Microscopy Listserve" {microscopy-at-microscopy.com} 13, 26 -- Subject: Call for Papers - Inter/Micro 2016 13, 26 -- Date: Wed, 24 Feb 2016 10:21:04 -0500 13, 26 -- Message-ID: {002301d16f16$fa107040$ee3150c0$-at-sprynet.com} 13, 26 -- MIME-Version: 1.0 13, 26 -- Content-Type: text/plain; 13, 26 -- charset="us-ascii" 13, 26 -- Content-Transfer-Encoding: 7bit 13, 26 -- X-Mailer: Microsoft Outlook 14.0 13, 26 -- Thread-Index: AdFvFobPmzC92okxRByiyEo5xFxtfA== 13, 26 -- Content-Language: en-us 13, 26 -- X-ELNK-Trace: 6888e50b2be9b4fee5331016acda17f99cb45db0fb6b68c30031faad74a9ee41350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c 13, 26 -- X-Originating-IP: 68.58.112.163 ==============================End of - Headers==============================
We are getting rid of our Denton Vacuum Evaporator DV-502. If anyone out there would like a free vacuum evaporator you are welcome to come and get it. Our lab is located at UNMC in Omaha, Nebraska.
Tom Bargar UNMC Electron Microscopy Core Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
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==============================Original Headers============================== 6, 37 -- From tbargar-at-unmc.edu Wed Feb 24 11:15:11 2016 6, 37 -- Received: from zixvpm02.unmc.edu (mysecuremail.unmc.edu [192.198.54.127]) 6, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1OHFBjx009521 6, 37 -- for {Microscopy-at-microscopy.com} ; Wed, 24 Feb 2016 11:15:11 -0600 6, 37 -- Received: from 127.0.0.1 (ZixVPM [127.0.0.1]) 6, 37 -- by Outbound.unmc.edu (Proprietary) with SMTP id C5E4638520B 6, 37 -- for {Microscopy-at-microscopy.com} ; Wed, 24 Feb 2016 10:55:53 -0600 (CST) 6, 37 -- Received: from UNMCEX1.unmcresforest.org (unknown [10.8.3.1]) 6, 37 -- (using TLSv1 with cipher AES128-SHA (128/128 bits)) 6, 37 -- (No client certificate requested) 6, 37 -- by zixvpm02.unmc.edu (Proprietary) with ESMTPS id BE04B38520A 6, 37 -- for {Microscopy-at-microscopy.com} ; Wed, 24 Feb 2016 10:55:52 -0600 (CST) 6, 37 -- Received: from UNMCEX2.unmcresforest.org ([fe80::151d:89d:49b6:a0ab]) by 6, 37 -- UNMCEX1.unmcresforest.org ([fe80::2023:ee90:3fae:f0d6%12]) with mapi id 6, 37 -- 14.03.0235.001; Wed, 24 Feb 2016 11:14:53 -0600 6, 37 -- From: "Bargar, Tom W" {tbargar-at-unmc.edu} 6, 37 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 6, 37 -- Subject: Free Denton Vacuum Evaporator DV-502 6, 37 -- Thread-Topic: Free Denton Vacuum Evaporator DV-502 6, 37 -- Thread-Index: AdFvJn+TTJy4rbS3ToC6BhV9WE0Qtw== 6, 37 -- Date: Wed, 24 Feb 2016 17:14:53 +0000 6, 37 -- Message-ID: {E5858BD0583CA8499131AC34AD6766B0AE854567-at-UNMCEX2.unmcresforest.org} 6, 37 -- Accept-Language: en-US 6, 37 -- Content-Language: en-US 6, 37 -- X-MS-Has-Attach: 6, 37 -- X-MS-TNEF-Correlator: 6, 37 -- x-originating-ip: [10.8.64.15] 6, 37 -- Content-Type: text/plain; charset="us-ascii" 6, 37 -- MIME-Version: 1.0 6, 37 -- X-VPM-MSG-ID: e26c73e3-4d1f-4bba-8481-375263525094 6, 37 -- X-VPM-HOST: zixvpm02.unmc.edu 6, 37 -- X-VPM-GROUP-ID: 9a1af4c2-901d-4ff8-951f-23599a9bbf45 6, 37 -- X-VPM-ENC-REGIME: ZixVPM,Plaintext 6, 37 -- X-VPM-CERT-FLAG: 0 6, 37 -- X-VPM-IS-HYBRID: 0 6, 37 -- Content-Transfer-Encoding: 8bit 6, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u1OHFBjx009521 ==============================End of - Headers==============================
I would like to hear privately from anyone using Tescan SEM and especially anyone who is using a Tescan with the integrated Gatan ultramicrotome for serial block face imaging. I'm not familiar with this company, so all feedback would be appreciated.
Tom Bargar UNMC Electron Microscopy Core Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
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==============================Original Headers============================== 7, 37 -- From tbargar-at-unmc.edu Thu Feb 25 10:27:10 2016 7, 37 -- Received: from zixvpm01.unmc.edu (zixvpm01.unmc.edu [192.198.54.126]) 7, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1PGRAaF005835 7, 37 -- for {Microscopy-at-microscopy.com} ; Thu, 25 Feb 2016 10:27:10 -0600 7, 37 -- Received: from 127.0.0.1 (ZixVPM [127.0.0.1]) 7, 37 -- by Outbound.unmc.edu (Proprietary) with SMTP id C11455C6181 7, 37 -- for {Microscopy-at-microscopy.com} ; Thu, 25 Feb 2016 10:04:19 -0600 (CST) 7, 37 -- Received: from UNMCEX3.unmcresforest.org (unknown [10.8.3.3]) 7, 37 -- (using TLSv1 with cipher AES128-SHA (128/128 bits)) 7, 37 -- (No client certificate requested) 7, 37 -- by zixvpm01.unmc.edu (Proprietary) with ESMTPS id D36265C6173 7, 37 -- for {Microscopy-at-microscopy.com} ; Thu, 25 Feb 2016 10:04:18 -0600 (CST) 7, 37 -- Received: from UNMCEX2.unmcresforest.org ([fe80::151d:89d:49b6:a0ab]) by 7, 37 -- UNMCEX3.unmcresforest.org ([fe80::9840:65e3:a657:ff93%13]) with mapi id 7, 37 -- 14.03.0235.001; Thu, 25 Feb 2016 10:26:55 -0600 7, 37 -- From: "Bargar, Tom W" {tbargar-at-unmc.edu} 7, 37 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 7, 37 -- Subject: would like to hear from Tescan customers 7, 37 -- Thread-Topic: would like to hear from Tescan customers 7, 37 -- Thread-Index: AdFv6Owu3Y0nkb+lTEWu6gBu4tHd9g== 7, 37 -- Date: Thu, 25 Feb 2016 16:26:54 +0000 7, 37 -- Message-ID: {E5858BD0583CA8499131AC34AD6766B0AE8549F1-at-UNMCEX2.unmcresforest.org} 7, 37 -- Accept-Language: en-US 7, 37 -- Content-Language: en-US 7, 37 -- X-MS-Has-Attach: 7, 37 -- X-MS-TNEF-Correlator: 7, 37 -- x-originating-ip: [10.8.64.15] 7, 37 -- Content-Type: text/plain; charset="us-ascii" 7, 37 -- MIME-Version: 1.0 7, 37 -- X-VPM-MSG-ID: e96d80d7-3a70-4386-a1c0-04b659029cc0 7, 37 -- X-VPM-HOST: zixvpm01.unmc.edu 7, 37 -- X-VPM-GROUP-ID: 0d5882fa-6463-4dbf-b262-a87cdbd7a309 7, 37 -- X-VPM-ENC-REGIME: ZixVPM,Plaintext 7, 37 -- X-VPM-CERT-FLAG: 0 7, 37 -- X-VPM-IS-HYBRID: 0 7, 37 -- Content-Transfer-Encoding: 8bit 7, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u1PGRAaF005835 ==============================End of - Headers==============================
What are the typical software systems you use to manage on-line booking to use scopes or sample prep facilities? Either commercially available or free systems are of my interest. I would be extremely grateful if you would please share with me the experiences and expectations you have had. The system we use now allows on-line booking of a dozen resources from users, notifying users in advance, managing the cost of the usage automatically, etc. However, it's not embedded within individual equipment. The booking and the usage is not electronically linked. Ideally when a booking is made to use a scope, and this user account will implement the booking by logging in the facility on the session. Therefore the booking and the real usage will be directly linked with a true picture. Anybody is using such a system now?
Best regards, Dr Z Zhou Loughborough University, UK
==============================Original Headers============================== 4, 62 -- From Z.Zhou-at-lboro.ac.uk Fri Feb 26 08:23:05 2016 4, 62 -- Received: from mta-1.lboro.ac.uk (mta-1.lut.ac.uk [158.125.160.47]) 4, 62 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1QEN4xS031468 4, 62 -- for {Microscopy-at-microscopy.com} ; Fri, 26 Feb 2016 08:23:05 -0600 4, 62 -- Received: from [158.125.167.11] (helo=ITSCASH-4.lunet.lboro.ac.uk) 4, 62 -- by mta-1.lboro.ac.uk with esmtps (TLSv1.2:ECDHE-RSA-AES256-SHA384:256) 4, 62 -- (Exim 4.86) 4, 62 -- id 1aZJI2-0003KK-IE 4, 62 -- for Microscopy-at-microscopy.com; Fri, 26 Feb 2016 14:22:42 +0000 4, 62 -- Received: from EUR01-HE1-obe.outbound.protection.outlook.com (213.199.154.207) 4, 62 -- by email.lboro.ac.uk (158.125.167.4) with Microsoft SMTP Server (TLS) id 4, 62 -- 14.3.266.1; Fri, 26 Feb 2016 14:23:01 +0000 4, 62 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=lunet.onmicrosoft.com; 4, 62 -- s=selector1-lboro-ac-uk; 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A PostDoc position is available in the BioEM Lab of the University of Basel, Switzerland.
We are looking for a person with experience in single particle cryo-EM and image processing, using RELION, FREALIGN, EMAN2, and other packages. The position is available immediately, and funded initially for two years, with the possibility for multi-year extension and possible transition into a permanent position. The role of this person should be to perform cryo-EM sample preparation and cryo-EM data collection, and perform advanced image processing and 3D reconstructions. Expertise in single particle image analysis is required. Several high-impact biological projects are waiting, including studying larger membrane protein complexes by cryo-EM as single particles, as well as helical assemblies.
The person should serve as liaison between the BioEM Lab, which is a newly established high-resolution cryo-EM service facility at the the Center for Cellular Imaging and NanoAnalytics of the Univeristy of Basel, and the laboratory of biomolecular research at the Paul-Scherrer Institute (PSI) in Villigen. An interaction with collaborators and clients from the pharmaceutical industry interested in membrane protein structure determination is also foreseen.
This position involves high-resolution cryo-EM structural work on membrane proteins, for which our FEI Titan Krios (Quantum-LS GIF / K2 Summit) and the FEI Polara (K2 Summit) are outstanding instruments. We also operate a number of additional instruments for sample screening and cryo-EM grid analysis, including a FEI Talos, CM200FEG, T12, CM100, CM10, Versa3D, and others. Large computer clusters are available. The lab atmosphere is especially nice, and the city of Basel in Switzerland at the border to Germany and France has a beautiful historic center and a rich culture, and provides a high standard of living. This is a good place to live and to do science.
If you are interested, please contact Henning.Stahlberg-at-unibas.ch for further infos.
Henning Stahlberg, PhD Prof. for Structural Biology, C-CINA, Biozentrum, University Basel Mattenstrasse 26 | D-BSSE | WRO-1058 | CH-4058 Basel | Switzerland http://c-cina.org | Tel. +41-61-387 32 62
==============================Original Headers============================== 11, 35 -- From henning.stahlberg-at-unibas.ch Sat Feb 27 03:26:18 2016 11, 35 -- Received: from mx1-priv.urz.unibas.ch (mx1-priv.urz.unibas.ch [131.152.226.164]) 11, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1R9QHmM021105 11, 35 -- for {Microscopy-at-microscopy.com} ; Sat, 27 Feb 2016 03:26:18 -0600 11, 35 -- Received: from localhost (localhost [127.0.0.1]) 11, 35 -- by mx1-priv.urz.unibas.ch (Postfix) with ESMTP id B15F23E0171 11, 35 -- for {Microscopy-at-microscopy.com} ; Sat, 27 Feb 2016 10:26:02 +0100 (CET) 11, 35 -- X-Virus-Scanned: amavisd-new at unibas.ch 11, 35 -- Received: from mx1-priv.urz.unibas.ch ([131.152.226.164]) 11, 35 -- by localhost (mx1-mgnt.urz.unibas.ch [127.0.0.1]) (amavisd-new, port 10024) 11, 35 -- with LMTP id psnNyuW6_AVh for {Microscopy-at-microscopy.com} ; 11, 35 -- Sat, 27 Feb 2016 10:26:02 +0100 (CET) 11, 35 -- Received: from URZ-HT-CAS-1.urz.unibas.ch (urz-ht-cas-1.urz.unibas.ch [131.152.8.131]) 11, 35 -- by mx1-priv.urz.unibas.ch (Postfix) with ESMTPS id A1F133E0185 11, 35 -- for {Microscopy-at-microscopy.com} ; Sat, 27 Feb 2016 10:26:02 +0100 (CET) 11, 35 -- Received: from URZ-MBX-1.urz.unibas.ch ([131.152.8.141]) by 11, 35 -- URZ-HT-CAS-1.urz.unibas.ch ([fe80::f8bd:7aba:d322:ad34%12]) with mapi id 11, 35 -- 14.03.0266.001; Sat, 27 Feb 2016 10:26:02 +0100 11, 35 -- From: Henning Stahlberg {henning.stahlberg-at-unibas.ch} 11, 35 -- To: Microscopy {Microscopy-at-microscopy.com} 11, 35 -- Subject: PostDoc in cryo-EM and image processing in Basel, Switzerland 11, 35 -- Thread-Topic: PostDoc in cryo-EM and image processing in Basel, Switzerland 11, 35 -- Thread-Index: AQHRcUDfmNVu3pRwuU6BdfawFhkB5Q== 11, 35 -- Date: Sat, 27 Feb 2016 09:26:01 +0000 11, 35 -- Message-ID: {5C666C53-43FE-408E-A676-74146D262FF7-at-unibas.ch} 11, 35 -- Accept-Language: en-US, de-CH 11, 35 -- Content-Language: en-US 11, 35 -- X-MS-Has-Attach: 11, 35 -- X-MS-TNEF-Correlator: 11, 35 -- x-originating-ip: [131.152.226.242] 11, 35 -- Content-Type: text/plain; charset="us-ascii" 11, 35 -- Content-ID: {EBFB11FC3A4BAF469B9A3584707F0FAC-at-zuv.ads.unibas.ch} 11, 35 -- MIME-Version: 1.0 11, 35 -- Content-Transfer-Encoding: 8bit 11, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u1R9QHmM021105 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both htowbin-at-amnh.org as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: htowbin-at-amnh.org Name: Henry Towbin
Organization: American Museum of Natural History
Title-Subject: [Filtered] Biological X-ray Analysis with SEM EDS
Message: Hi All, Can anyone provide some referenced for good biological thin film x-ray analysis? I am particularly interested in how to prepare good standards for using the Hall-Marshal or similar method to analyze C, N and P. I am trying to help some colleague with analyzing the compositions of singles celled organisms with x-ray analysis in an SEM. They are attempting to dry the cells on a formvar support and plan to measure counts from the cell and the film and then subtract the counts of the film to calculate composition. I am highly skeptical of the methods they want to use from Norland et al. 1995 (Light Element Analysis of Individual Bacteria by X-Ray Microanalysis), so any advice would be much appreciated. Thank you, Henry
Norland et al. 1995, Light Element Analysis of Individual Bacteria by X-Ray Microanalysis, http://aem.asm.org/content/61/4/1357.full.pdf ______________________________ Henry Towbin Laboratory Co-manager Microscopy and Imaging Facility American Museum of Natural History 79th St & Central Park West NY, NY 10024
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Announcing a MAS Topical Conference, Electron Probe Microanalysis 2016 May 16-19, 2016 at the University of Wisconsin-Madison
STUDENT FINANCIAL SUPPORT AVAILABLE!
TOPICAL CONFERENCE PROGRAM -Practical microanalysis for beginners to professionals -Plenary Conference including user meetings, tutorials, group discussion, problem solving, and instrument demos -Quantitative analysis using the electron microprobe and SEM with EDS and WDS -Improvements in microanalysis from measurement to accuracy -Trace element analysis -Compositional x-ray mapping and cathodoluminescence -Applications in earth science, materials science, and industry
STUDENT FINANCIAL SUPPORT AVAILABLE -Early career scholars: undergraduate, graduate, postdoc, and early career professionals may apply -Priority will be given to those with co-sponsorship from a university, advisor, or employer -ECS awardees may submit a 2-page abstract for platform or poster presentation. -Awardees will receive a maximum of $750 reimbursement for travel, lodging, and registration.
DEADLINE MARCH 15, 2016 FOR ABSTRACTS AND ECS APPLICATIONS
Please visit for more information http://www.microprobe.org/topical-conferences/epma-2016-1/epma-2016
The Electron Microscopy Laboratory at the Frederick National Laboratory for Cancer Research, located in Frederick, Maryland, has an open position of Research Associate I. Main duties will include: 1) biological sample preparation for electron microscopy (negative staining, plastic embedding, vitrification for cryo-EM, other approaches as necessary), 2) operation of electron microscopes for imaging and data collection, and 3) analysis and presentation of data. If interested, please apply directly using the following link:
Yaroslav Tsybovsky (Contractor) Scientist Frederick National Laboratory for Cancer Research Leidos Biomedical Research, Inc. Post Office Box B Frederick, Maryland 21702
==============================Original Headers============================== 7, 47 -- From yaroslav.tsybovsky-at-nih.gov Thu May 12 12:04:23 2016 7, 47 -- Received: from nihxway3out.hub.nih.gov (nihxway3out.hub.nih.gov [128.231.90.111]) 7, 47 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u4CH4NKn001027 7, 47 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2016 12:04:23 -0500 7, 47 -- X-SBRS-Extended: Low 7, 47 -- X-IronPortListener: Outbound_SMTP 7, 47 -- IronPort-PHdr: =?us-ascii?q?9a23=3AGVHDpBbZhvUgcMpc3gRB9OH/LSx+4OfEezUN459i?= 7, 47 -- =?us-ascii?q?sYplN5qZpM66bnLW6fgltlLVR4KTs6sC0LqH9fmxEjVYvN6oizMrTt9lb1c9k8?= 7, 47 -- =?us-ascii?q?IYnggtUoauKHbQC7rUVRE8B9lIT1R//nu2YgB/Ecf6YEDO8DXptWZBUiv2OQc9?= 7, 47 -- =?us-ascii?q?HOnpAIma153xjLDjvcSCKFwQ2XKUWvBbElaflU3prM4YgI9veO4a6yDihT92Qd?= 7, 47 -- =?us-ascii?q?lQ3n5iPlmJnhzxtY+a9Z9n9DlM6bp6r5YTGZjge+wEaZAQTHF/ayFmrPHs4FPm?= 7, 47 -- =?us-ascii?q?TACV4WAXVX0Huh9JCBLC9xr9Roa3uSz//KIp/SiRJ8rtRrcsSByn7qxxTwTjjz?= 7, 47 -- =?us-ascii?q?8WcTU+9TeEpNZ3ifcRnAmwrRth2I3FJMmkPeB5ZafUY5taY0thGeB6dm0JMaiJ?= 7, 47 -- =?us-ascii?q?JcNbHuMbOv1cppe7u0AfpxygHgq9LOXuynlHgWGgjv5y6PgoDQyThF9oJNkJqn?= 7, 47 -- =?us-ascii?q?mB6YytOQ=3D=3D?= 7, 47 -- X-IronPort-Anti-Spam-Filtered: true 7, 47 -- X-IronPort-Anti-Spam-Result: =?us-ascii?q?A2BmAgAetzRX/4JHKJxegzhVfwW5WQENg?= 7, 47 -- =?us-ascii?q?XYkhXACgTY4FAEBAQEBAQEBZBwLgi2CHBIoUQEVFRQFPSYBBBsaiA0FCZwOhTq?= 7, 47 -- =?us-ascii?q?bZIYmiQyDKYIuBZgnAYV9iCCBaU6EAYMehUECj0EeAQFCgTYMdIE1iCABfgEBA?= 7, 47 -- =?us-ascii?q?Q?= 7, 47 -- X-IPAS-Result: =?us-ascii?q?A2BmAgAetzRX/4JHKJxegzhVfwW5WQENgXYkhXACgTY4FAE?= 7, 47 -- =?us-ascii?q?BAQEBAQEBZBwLgi2CHBIoUQEVFRQFPSYBBBsaiA0FCZwOhTqbZIYmiQyDKYIuB?= 7, 47 -- =?us-ascii?q?ZgnAYV9iCCBaU6EAYMehUECj0EeAQFCgTYMdIE1iCABfgEBAQ?= 7, 47 -- X-IronPort-AV: E=Sophos;i="5.24,610,1454994000"; 7, 47 -- d="scan'208";a="459727113" 7, 47 -- Received: from unknown (HELO msgb10.nih.gov) ([156.40.71.130]) 7, 47 -- by nihxway3out.hub.nih.gov with ESMTP/TLS/AES256-SHA; 12 May 2016 13:04:02 -0400 7, 47 -- Received: from MSGB01.nih.gov ([169.254.1.154]) by msgb10.nih.gov 7, 47 -- ([169.254.10.4]) with mapi id 14.03.0279.002; Thu, 12 May 2016 13:04:02 -0400 7, 47 -- From: "Tsybovsky, Yaroslav (NIH/NCI) [C]" {yaroslav.tsybovsky-at-nih.gov} 7, 47 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 7, 47 -- Subject: TEM: Research Associate position at Leidos Biomedical Research, 7, 47 -- Inc./Frederick National Laboratory for Cancer Research 7, 47 -- Thread-Topic: TEM: Research Associate position at Leidos Biomedical 7, 47 -- Research, Inc./Frederick National Laboratory for Cancer Research 7, 47 -- Thread-Index: AdGscBobBV8ZFKKkTvmemwETdQ92zg== 7, 47 -- Date: Thu, 12 May 2016 17:04:01 +0000 7, 47 -- Message-ID: {AEB1E864C83965459AE2D8A2139E1B2DD13FE7-at-msgb01.nih.gov} 7, 47 -- Accept-Language: en-US 7, 47 -- Content-Language: en-US 7, 47 -- X-MS-Has-Attach: 7, 47 -- X-MS-TNEF-Correlator: 7, 47 -- x-originating-ip: [156.40.70.6] 7, 47 -- Content-Type: text/plain; charset="us-ascii" 7, 47 -- MIME-Version: 1.0 7, 47 -- Content-Transfer-Encoding: 8bit 7, 47 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u4CH4NKn001027 ==============================End of - Headers==============================
The Electron Microscopy Laboratory at the Frederick National Laboratory for Cancer Research, located in Frederick, Maryland, has an open position of Research Associate I. Main duties will include: 1) biological sample preparation for electron microscopy (negative staining, plastic embedding, vitrification for cryo-EM, other approaches as necessary), 2) operation of electron microscopes for imaging and data collection, and 3) analysis and presentation of data. If interested, please apply directly using the following link:
Yaroslav Tsybovsky (Contractor) Scientist Frederick National Laboratory for Cancer Research Leidos Biomedical Research, Inc. Post Office Box B Frederick, Maryland 21702
==============================Original Headers============================== 7, 47 -- From yaroslav.tsybovsky-at-nih.gov Thu May 12 12:04:23 2016 7, 47 -- Received: from nihxway3out.hub.nih.gov (nihxway3out.hub.nih.gov [128.231.90.111]) 7, 47 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u4CH4NKn001027 7, 47 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2016 12:04:23 -0500 7, 47 -- X-SBRS-Extended: Low 7, 47 -- X-IronPortListener: Outbound_SMTP 7, 47 -- IronPort-PHdr: =?us-ascii?q?9a23=3AGVHDpBbZhvUgcMpc3gRB9OH/LSx+4OfEezUN459i?= 7, 47 -- =?us-ascii?q?sYplN5qZpM66bnLW6fgltlLVR4KTs6sC0LqH9fmxEjVYvN6oizMrTt9lb1c9k8?= 7, 47 -- =?us-ascii?q?IYnggtUoauKHbQC7rUVRE8B9lIT1R//nu2YgB/Ecf6YEDO8DXptWZBUiv2OQc9?= 7, 47 -- =?us-ascii?q?HOnpAIma153xjLDjvcSCKFwQ2XKUWvBbElaflU3prM4YgI9veO4a6yDihT92Qd?= 7, 47 -- =?us-ascii?q?lQ3n5iPlmJnhzxtY+a9Z9n9DlM6bp6r5YTGZjge+wEaZAQTHF/ayFmrPHs4FPm?= 7, 47 -- =?us-ascii?q?TACV4WAXVX0Huh9JCBLC9xr9Roa3uSz//KIp/SiRJ8rtRrcsSByn7qxxTwTjjz?= 7, 47 -- =?us-ascii?q?8WcTU+9TeEpNZ3ifcRnAmwrRth2I3FJMmkPeB5ZafUY5taY0thGeB6dm0JMaiJ?= 7, 47 -- =?us-ascii?q?JcNbHuMbOv1cppe7u0AfpxygHgq9LOXuynlHgWGgjv5y6PgoDQyThF9oJNkJqn?= 7, 47 -- =?us-ascii?q?mB6YytOQ=3D=3D?= 7, 47 -- X-IronPort-Anti-Spam-Filtered: true 7, 47 -- X-IronPort-Anti-Spam-Result: =?us-ascii?q?A2BmAgAetzRX/4JHKJxegzhVfwW5WQENg?= 7, 47 -- =?us-ascii?q?XYkhXACgTY4FAEBAQEBAQEBZBwLgi2CHBIoUQEVFRQFPSYBBBsaiA0FCZwOhTq?= 7, 47 -- =?us-ascii?q?bZIYmiQyDKYIuBZgnAYV9iCCBaU6EAYMehUECj0EeAQFCgTYMdIE1iCABfgEBA?= 7, 47 -- =?us-ascii?q?Q?= 7, 47 -- X-IPAS-Result: =?us-ascii?q?A2BmAgAetzRX/4JHKJxegzhVfwW5WQENgXYkhXACgTY4FAE?= 7, 47 -- =?us-ascii?q?BAQEBAQEBZBwLgi2CHBIoUQEVFRQFPSYBBBsaiA0FCZwOhTqbZIYmiQyDKYIuB?= 7, 47 -- =?us-ascii?q?ZgnAYV9iCCBaU6EAYMehUECj0EeAQFCgTYMdIE1iCABfgEBAQ?= 7, 47 -- X-IronPort-AV: E=Sophos;i="5.24,610,1454994000"; 7, 47 -- d="scan'208";a="459727113" 7, 47 -- Received: from unknown (HELO msgb10.nih.gov) ([156.40.71.130]) 7, 47 -- by nihxway3out.hub.nih.gov with ESMTP/TLS/AES256-SHA; 12 May 2016 13:04:02 -0400 7, 47 -- Received: from MSGB01.nih.gov ([169.254.1.154]) by msgb10.nih.gov 7, 47 -- ([169.254.10.4]) with mapi id 14.03.0279.002; Thu, 12 May 2016 13:04:02 -0400 7, 47 -- From: "Tsybovsky, Yaroslav (NIH/NCI) [C]" {yaroslav.tsybovsky-at-nih.gov} 7, 47 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 7, 47 -- Subject: TEM: Research Associate position at Leidos Biomedical Research, 7, 47 -- Inc./Frederick National Laboratory for Cancer Research 7, 47 -- Thread-Topic: TEM: Research Associate position at Leidos Biomedical 7, 47 -- Research, Inc./Frederick National Laboratory for Cancer Research 7, 47 -- Thread-Index: AdGscBobBV8ZFKKkTvmemwETdQ92zg== 7, 47 -- Date: Thu, 12 May 2016 17:04:01 +0000 7, 47 -- Message-ID: {AEB1E864C83965459AE2D8A2139E1B2DD13FE7-at-msgb01.nih.gov} 7, 47 -- Accept-Language: en-US 7, 47 -- Content-Language: en-US 7, 47 -- X-MS-Has-Attach: 7, 47 -- X-MS-TNEF-Correlator: 7, 47 -- x-originating-ip: [156.40.70.6] 7, 47 -- Content-Type: text/plain; charset="us-ascii" 7, 47 -- MIME-Version: 1.0 7, 47 -- Content-Transfer-Encoding: 8bit 7, 47 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u4CH4NKn001027 ==============================End of - Headers==============================
Note: SCUR Annual meeting will take place during the first 2 days of EMC16 (the latter will take place Aug, 28 - Sept, 2 in Lyon, France)
Interested in Skin Research - Ultrastructure -Morphology - Imaging techniques and other modern techniques in Dermatological / Dermatopathological Research & Science?
Dear Colleague(s),
It is still NOT TOO LATE to submit your abstract for presentation at the forthcoming 43rd Annual meeting of SCUR- the Skin Imaging Society, 29th-30th August 2016 in Lyon, France [cf. www.scur.org]
New deadline: May 18th 2016
NOTE: 4 Travel Grants of 200[euro] for junior scientists (students and researchers under 35 y. o.) and technicians, members of SCUR (either active members or prospective new members submitting SCUR membership application simultaneously).
THREE Awards *Best young scientist presentation (200 [euro]) *Best oral presentation (200 [euro]) *Best poster presentation (200 [euro])
Please, send your abstract to { { scur.lyon2016-at-gmail.com } } (find RTF form on website www.scur.org - and link then to 'Next SCUR Meeting' and find General information at: https://www.scur.org/content/e1174/e1181/e1182 ,
at the bottom of that page also: all forms [Announcement = Invitation to Lyon - Society for Cutaneous Ultrastructure Research, {abstract submission form} , {Social Events Registration Form} , {Membership Application Form} as well as the { TRAVEL GRANT APPLICATION FORM} ]
You can visit / attend SCUR sessions if you register for whole EMC-16 - or you register only for SCUR program. (BUT: 'Registering' also for {SCUR only} must be done via the EMC-16-Website = http://www.emc2016.fr/en/register/fees - Early Bird Registering until 1 June 2016 - FIND there (if you only want to attend the SCUR-Meeting) also the rate for:
"2 Days SCUR session only (29th & 30th August) [at a special reduced rate of ] [euro] 250 " )
End your summer vacation with an exciting stop at the { Gaules capital of Lugdunum} : so many things to see and to do, apart from the scientific sessions, at the heart of the {Beaujolais region} and the { famous French kitchen} ! (visit: http://www.onlylyon.com/en/ )
SCUR 2016 ALL INFORMATION:
Thanking you for support and further distribution of this message to other interested colleagues/persons.
Looking forward to meeting YOU and many other interested Colleagues there,
With best regards,
Wolfgang MUSS (also Member of MSA) Secretary of SCUR - The Skin Imaging Society SALZBURG-AUSTRIA
==============================Original Headers============================== 27, 20 -- From wij.muss-at-aon.at Thu May 12 05:58:01 2016 27, 20 -- Received: from bsmtp2.bon.at (bsmtp2.bon.at [213.33.87.16]) 27, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u4CAw0SJ015148 27, 20 -- for {microscopy-at-microscopy.com} ; Thu, 12 May 2016 05:58:01 -0500 27, 20 -- Received: from MussTHINK (unknown [93.83.25.98]) 27, 20 -- by bsmtp2.bon.at (Postfix) with ESMTPSA id 3r592C6kH6z5tlP 27, 20 -- for {microscopy-at-microscopy.com} ; Thu, 12 May 2016 12:58:11 +0200 (CEST) 27, 20 -- From: "MUSS Wolfgang SCUR Secretary" {wij.muss-at-aon.at} 27, 20 -- To: {microscopy-at-microscopy.com} 27, 20 -- Subject: Last announcement - Abstract submission deadline extended to 18th May 2016 43rd SCUR-The SKIN IMAGING SOCIETY-Annual Meeting, LYON,29-30th August 2016 27, 20 -- Date: Thu, 12 May 2016 12:58:14 +0200 27, 20 -- Message-ID: {009101d1ac3d$2f090d80$8d1b2880$-at-aon.at} 27, 20 -- MIME-Version: 1.0 27, 20 -- Content-Type: text/plain; 27, 20 -- charset="UTF-8" 27, 20 -- X-Mailer: Microsoft Outlook 15.0 27, 20 -- Thread-Index: AdGsOXYGBS1uhIMnQHavjW1y8kY0Xw== 27, 20 -- Content-Language: de-at 27, 20 -- Content-Transfer-Encoding: 8bit 27, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u4CAw0SJ015148 ==============================End of - Headers==============================
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Email: ucsb.materials-at-gmail.com Name: Dawn McTague
Organization: University of California, Santa Barbara
Title-Subject: [Filtered] Job Opening at UCSB: TEM Engineer
Message: The University of California Santa Barbara Materials Department has an immediate opening for a TEM Microscopy Engineer. This position is responsible for autonomously managing the training, maintenance, operations, and research in transmission electron microscopy (TEM), atom probe microscopy, and focused ion beam (FIB) technology. This position involves high-level collaborative research with faculty, students, and research personnel in Materials and other campus units utilizing the facility. The position also provides training and instruction to students, research personnel, and outside users in advanced techniques of transmission electron microscopy in addition to maintenance and upkeep of advanced equipment. Incumbent must have a strong background in research microscopy with a high level of expertise in TEM microscopy. Experience working in a fast-paced research environment with a diverse population combined with an ability to develop thorough training programs is required. Ph.D. preferred.
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Sorry, wrong dates: August 22-27, 2016. (Lectures August 23-26).
Henning.
Henning Stahlberg, PhD Prof. for Structural Biology, C-CINA, Biozentrum, University Basel Mattenstrasse 26 | D-BSSE | WRO-1058 | CH-4058 Basel | Switzerland http://c-cina.org | Tel. +41-61-387 32 62
On 11 May 2016, at 10:49, Henning Stahlberg {henning.stahlberg-at-unibas.ch} wrote:
Dear colleagues,
We are happy to announce the fifth 2DX Workshop on Electron Crystallography of Membrane Proteins, which will take place at the University of Basel (Switzerland), on August 22-27, 2016. The workshop will provide hands-on training on practical aspects of 2D crystal data collection on a Titan Krios, and cover in depth the theoretical foundations and the practical image processing of 2D crystal cryo-EM images with the 2DX software package. No previous experience is required.
Topics include: Basics of 2D electron crystallography * Introduction to 2D electron crystallography * Cryo-electron microscopy sample preparation for 2D crystals of membrane proteins * Cryo-EM data collection of 2D crystals * Introduction to 2DX * Drift correction using ZORRO Basic image processing with 2DX * Determination of the defocus, crystal tilt geometry, crystal lattice * Crystal Unbending - including movie-mode drift correction of individual unit cells in dose-fractionated image series * 2D merging and 3D merging with reconstruction via weighted back projection * Automation of the processing pipeline Advanced topics in image processing with 2DX * Approaches for a partial reconstruction of amplitudes and phases inside of the missing cone * Processing of 2D crystal unit cells with RELION and FREALIGN, under exploitation of neighborhood correlation of certain image parameters * Advanced usage of the 2DX backend library * Preparation of final results with quantitative description of statistical values (e.g., resolution) for export to databases (EMDB).
Computing infrastructure will be provided in place and participants are welcome to bring their own data sets and/or computer (OSX or Linux). More information can be found at http://www.2dx.unibas.ch/workshop/2016
For registration, please include information about your affiliation, and a short motivation statement (max 200 words). The registration fee is 250 CHF (travel and accommodation is not included), registration deadline is June 30th. As we can only provide a limited number of workstations, we typically accept participants on a "first come - first serve" basis. Please do not hesitate to contact us for any questions. Looking forward to welcoming you in Basel,
Nikhil Biyani, Ricardo Righetto, Robert McLeod, Henning Stahlberg.
Henning Stahlberg, PhD Prof. for Structural Biology, C-CINA, Biozentrum, University Basel Mattenstrasse 26 | D-BSSE | WRO-1058 | CH-4058 Basel | Switzerland http://c-cina.org | Tel. +41-61-387 32 62
==============================Original Headers============================== 9, 41 -- From henning.stahlberg-at-unibas.ch Wed May 11 06:30:24 2016 9, 41 -- Received: from mx2-priv.urz.unibas.ch (mx2-priv.urz.unibas.ch [131.152.226.165]) 9, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u4BBUNAK005765 9, 41 -- for {Microscopy-at-microscopy.com} ; Wed, 11 May 2016 06:30:23 -0500 9, 41 -- Received: from localhost (localhost [127.0.0.1]) 9, 41 -- by mx2-priv.urz.unibas.ch (Postfix) with ESMTP id 156422201A8 9, 41 -- for {Microscopy-at-microscopy.com} ; Wed, 11 May 2016 13:30:31 +0200 (CEST) 9, 41 -- X-Virus-Scanned: amavisd-new at unibas.ch 9, 41 -- Received: from mx2-priv.urz.unibas.ch ([131.152.226.165]) 9, 41 -- by localhost (mx2-mgnt.urz.unibas.ch [127.0.0.1]) (amavisd-new, port 10024) 9, 41 -- with LMTP id koKeezvvHJfO for {Microscopy-at-microscopy.com} ; 9, 41 -- Wed, 11 May 2016 13:30:31 +0200 (CEST) 9, 41 -- Received: from URZ-HT-CAS-1.urz.unibas.ch (urz-ht-cas-1.urz.unibas.ch [131.152.8.131]) 9, 41 -- by mx2-priv.urz.unibas.ch (Postfix) with ESMTPS id 03F652201A2 9, 41 -- for {Microscopy-at-microscopy.com} ; Wed, 11 May 2016 13:30:31 +0200 (CEST) 9, 41 -- Received: from URZ-MBX-1.urz.unibas.ch ([131.152.8.141]) by 9, 41 -- URZ-HT-CAS-1.urz.unibas.ch ([fe80::f8bd:7aba:d322:ad34%12]) with mapi id 9, 41 -- 14.03.0279.002; Wed, 11 May 2016 13:30:30 +0200 9, 41 -- From: Henning Stahlberg {henning.stahlberg-at-unibas.ch} 9, 41 -- To: Microscopy {Microscopy-at-microscopy.com} 9, 41 -- CC: Ricardo Diogo Righetto {ricardo.righetto-at-unibas.ch} , 9, 41 -- Robert McLeod 9, 41 -- {robert.mcleod-at-unibas.ch} , 9, 41 -- Nikhil Biyani {nikhil.biyani-at-unibas.ch} 9, 41 -- Subject: Workshop announcement: Electron crystallography of 2D crystals of 9, 41 -- membrane proteins with 2DX, August 22-27, 2016 9, 41 -- Thread-Topic: Workshop announcement: Electron crystallography of 2D crystals 9, 41 -- of membrane proteins with 2DX, August 22-27, 2016 9, 41 -- Thread-Index: AQHRq3iDqHuL+mRb9Uuzz6r21q4Q2Q== 9, 41 -- Date: Wed, 11 May 2016 11:30:25 +0000 9, 41 -- Message-ID: {AD4DFA52-B645-4E6E-A86B-39E8E9A85A36-at-unibas.ch} 9, 41 -- Accept-Language: en-US, de-CH 9, 41 -- Content-Language: en-US 9, 41 -- X-MS-Has-Attach: 9, 41 -- X-MS-TNEF-Correlator: 9, 41 -- x-originating-ip: [131.152.226.242] 9, 41 -- Content-Type: text/plain; charset="utf-8" 9, 41 -- Content-ID: {4AFC68C688518C448406D6EFD0DEB3B6-at-zuv.ads.unibas.ch} 9, 41 -- MIME-Version: 1.0 9, 41 -- Content-Transfer-Encoding: 8bit 9, 41 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id u4BBUNAK005765 ==============================End of - Headers==============================
We are happy to announce the fifth 2DX Workshop on Electron Crystallography of Membrane Proteins, which will take place at the University of Basel (Switzerland), on August 22-27, 2016. The workshop will provide hands-on training on practical aspects of 2D crystal data collection on a Titan Krios, and cover in depth the theoretical foundations and the practical image processing of 2D crystal cryo-EM images with the 2DX software package. No previous experience is required.
Topics include: Basics of 2D electron crystallography * Introduction to 2D electron crystallography * Cryo-electron microscopy sample preparation for 2D crystals of membrane proteins * Cryo-EM data collection of 2D crystals * Introduction to 2DX * Drift correction using ZORRO Basic image processing with 2DX * Determination of the defocus, crystal tilt geometry, crystal lattice * Crystal Unbending - including movie-mode drift correction of individual unit cells in dose-fractionated image series * 2D merging and 3D merging with reconstruction via weighted back projection * Automation of the processing pipeline Advanced topics in image processing with 2DX * Approaches for a partial reconstruction of amplitudes and phases inside of the missing cone * Processing of 2D crystal unit cells with RELION and FREALIGN, under exploitation of neighborhood correlation of certain image parameters * Advanced usage of the 2DX backend library * Preparation of final results with quantitative description of statistical values (e.g., resolution) for export to databases (EMDB).
Computing infrastructure will be provided in place and participants are welcome to bring their own data sets and/or computer (OSX or Linux). More information can be found at http://www.2dx.unibas.ch/workshop/2016
For registration, please include information about your affiliation, and a short motivation statement (max 200 words). The registration fee is 250 CHF (travel and accommodation is not included), registration deadline is June 30th. As we can only provide a limited number of workstations, we typically accept participants on a "first come - first serve" basis. Please do not hesitate to contact us for any questions. Looking forward to welcoming you in Basel,
Nikhil Biyani, Ricardo Righetto, Robert McLeod, Henning Stahlberg.
Henning Stahlberg, PhD Prof. for Structural Biology, C-CINA, Biozentrum, University Basel Mattenstrasse 26 | D-BSSE | WRO-1058 | CH-4058 Basel | Switzerland http://c-cina.org | Tel. +41-61-387 32 62
==============================Original Headers============================== 9, 41 -- From henning.stahlberg-at-unibas.ch Wed May 11 03:49:28 2016 9, 41 -- Received: from mx1-priv.urz.unibas.ch (mx1-priv.urz.unibas.ch [131.152.226.164]) 9, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u4B8nR35005006 9, 41 -- for {Microscopy-at-microscopy.com} ; Wed, 11 May 2016 03:49:28 -0500 9, 41 -- Received: from localhost (localhost [127.0.0.1]) 9, 41 -- by mx1-priv.urz.unibas.ch (Postfix) with ESMTP id 19E063E01F9 9, 41 -- for {Microscopy-at-microscopy.com} ; Wed, 11 May 2016 10:49:35 +0200 (CEST) 9, 41 -- X-Virus-Scanned: amavisd-new at unibas.ch 9, 41 -- Received: from mx1-priv.urz.unibas.ch ([131.152.226.164]) 9, 41 -- by localhost (mx1-mgnt.urz.unibas.ch [127.0.0.1]) (amavisd-new, port 10024) 9, 41 -- with LMTP id U7Dv0uAVLwUf for {Microscopy-at-microscopy.com} ; 9, 41 -- Wed, 11 May 2016 10:49:34 +0200 (CEST) 9, 41 -- Received: from URZ-HT-CAS-4.urz.unibas.ch (urz-ht-cas-4.urz.unibas.ch [131.152.8.134]) 9, 41 -- by mx1-priv.urz.unibas.ch (Postfix) with ESMTPS id ABBFC3E0296 9, 41 -- for {Microscopy-at-microscopy.com} ; Wed, 11 May 2016 10:49:25 +0200 (CEST) 9, 41 -- Received: from URZ-MBX-1.urz.unibas.ch ([131.152.8.141]) by 9, 41 -- URZ-HT-CAS-4.urz.unibas.ch ([fe80::5431:4219:b242:4cca%12]) with mapi id 9, 41 -- 14.03.0279.002; Wed, 11 May 2016 10:49:25 +0200 9, 41 -- From: Henning Stahlberg {henning.stahlberg-at-unibas.ch} 9, 41 -- To: Microscopy {Microscopy-at-microscopy.com} 9, 41 -- CC: Nikhil Biyani {nikhil.biyani-at-unibas.ch} , 9, 41 -- Ricardo Diogo Righetto 9, 41 -- {ricardo.righetto-at-unibas.ch} , 9, 41 -- Robert McLeod {robert.mcleod-at-unibas.ch} 9, 41 -- Subject: Workshop announcement: Electron crystallography of 2D crystals of 9, 41 -- membrane proteins with 2DX, August 17-19. 9, 41 -- Thread-Topic: Workshop announcement: Electron crystallography of 2D crystals 9, 41 -- of membrane proteins with 2DX, August 17-19. 9, 41 -- Thread-Index: AQHRq2IFeqSWzMcRw0iz5Z6nMWDHlw== 9, 41 -- Date: Wed, 11 May 2016 08:49:25 +0000 9, 41 -- Message-ID: {D2C664AF-4F24-4E0D-BF2D-98895C061C72-at-unibas.ch} 9, 41 -- Accept-Language: en-US, de-CH 9, 41 -- Content-Language: en-US 9, 41 -- X-MS-Has-Attach: 9, 41 -- X-MS-TNEF-Correlator: 9, 41 -- x-originating-ip: [131.152.226.242] 9, 41 -- Content-Type: text/plain; charset="utf-8" 9, 41 -- Content-ID: {D08F1F509F7DAA49B3D4132654F1E341-at-zuv.ads.unibas.ch} 9, 41 -- MIME-Version: 1.0 9, 41 -- Content-Transfer-Encoding: 8bit 9, 41 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id u4B8nR35005006 ==============================End of - Headers==============================
Christopher J. Gilpin Ph.D. Director, Life Science Microscopy Facility Purdue University Whistler Hall of Agriculture Research, Room S052 170 S. University St West Lafayette, IN 47907 765-494-7750 gilpin-at-purdue.edu lsmf-at-purdue.edu reaches everyone in the facility
-----Original Message----- X-from: ajhall-at-prairienanotech.com [mailto:ajhall-at-prairienanotech.com] Sent: Tuesday, May 10, 2016 1:11 PM To: Gilpin, Christopher J {gilpin-at-purdue.edu}
Hi Microscopy Listerve and Shouliang Zhang,
This issue has been around for a long time. In general, university equipment is there to support the research efforts of their faculty and students. Commercial analytical labs exist which can serve industrial needs. University labs, though, are often tempted to sell their services to industry to make more money. A valid argument is often raised that this is unfair competition to those commercial labs because the (very expensive) university equipment most likely has been purchased with federal grants to support academic research. Some feel this means universities should not do industrial work at all, and others feel it's OK so long as they don't undercut the commercial labs, which could open you up to a lawsuit. Some of the big commercial labs are VERY aggressive about this!
Note (for full disclosure): I am with a small commercial service lab but also work in an academic setting.
Allen J. Hall Prairie Nanotechnology www.prairienanotech.com
==============================Original Headers============================== 4, 45 -- From ajhall-at-prairienanotech.com Tue May 10 11:50:41 2016 4, 45 -- Received: from mail-io0-f178.google.com (mail-io0-f178.google.com [209.85.223.178]) 4, 45 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u4AGofMY014855 4, 45 -- for {Microscopy-at-microscopy.com} ; Tue, 10 May 2016 11:50:41 -0500 4, 45 -- Received: by mail-io0-f178.google.com with SMTP id d62so23241089iof.2 4, 45 -- for {Microscopy-at-microscopy.com} ; Tue, 10 May 2016 09:50:47 -0700 (PDT) 4, 45 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 45 -- d=prairienanotech-com.20150623.gappssmtp.com; s=20150623; 4, 45 -- h=message-id:date:from:user-agent:mime-version:to:subject 4, 45 -- :content-transfer-encoding; 4, 45 -- bh=Plgywb2O1+ImPmxWFmcid2u5uNXP8Sirx3J2XesJPCs=; 4, 45 -- b=t2VgeSn9eeNcFDD2LLgMyflPSDj8b8Xq67qC+mzUypN33zLwJmoTXuHN0x+SjFXYAW 4, 45 -- v4X89t2Jd3S35wojKfAdAAhJI8hiVP46ZjhbCVPLOAzTa4wYOtQGwH23UYRxx/TfALN0 4, 45 -- zTrWblxCxB+O9wlMnlGMp6pLXgVzvMwQfopuvVvHWrDPrfve984uxJ07JYv1EhFO/4XT 4, 45 -- 2MpHY5RRS1SsHmtbF4soLrMBk84IS2xbnRPUvgp/ZmXcRQfCm3w6M0bPTuXEv4S+M6eS 4, 45 -- ACAZzAtMOq2YsAmMCTcDvRyx/+OUOwC9BGA02I0on0USdiPya3uHYZcTB+goErwLxQDZ 4, 45 -- 5ySw== 4, 45 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 45 -- d=1e100.net; s=20130820; 4, 45 -- h=x-gm-message-state:message-id:date:from:user-agent:mime-version:to 4, 45 -- :subject:content-transfer-encoding; 4, 45 -- bh=Plgywb2O1+ImPmxWFmcid2u5uNXP8Sirx3J2XesJPCs=; 4, 45 -- b=QFk4kWfMxbDcQtyn1C+gqxGkLC5+SjI0OA/LMHGxfEPxsP3x4V7K4CYXLufcFUVVLM 4, 45 -- c8akf4kBo8MsjTfzOGAgiFZkDsZBSJvogX2nUSazbTA7WyHvx1wqyeGothvxx0SozVg1 4, 45 -- jL3xVRlXGgGtiopZ649700HJWIg4fj2hU9+dXKRjL3ox8x9uJ3nbfOtXJ15rCrNzOfXs 4, 45 -- 1aJ7jZBrMPbIu3hq2RjvzF71eVnFf7xr/F6DJZH8TFn7bhOLCtuZ1Kkp9jVnQqGr9TMH 4, 45 -- 72aIVum6M85oHgNk9CoQGc3TkDYcqnzq+7LGykHyvwy7cTA9K/PSE/Z59z8fPwcwpTDC 4, 45 -- kCpA== 4, 45 -- X-Gm-Message-State: AOPr4FUkQov8olV3AjXGQ3Oz1CyaYwlE6ETR3r6lvbMv7pLDitpTN4n+LhJS933ONrVvDw== 4, 45 -- X-Received: by 10.107.147.138 with SMTP id v132mr41355964iod.38.1462899046675; 4, 45 -- Tue, 10 May 2016 09:50:46 -0700 (PDT) 4, 45 -- Received: from AllenH.local (c-98-212-86-40.hsd1.il.comcast.net. [98.212.86.40]) 4, 45 -- by smtp.googlemail.com with ESMTPSA id uy5sm1406989igc.4.2016.05.10.09.50.45 4, 45 -- for {Microscopy-at-microscopy.com} 4, 45 -- (version=TLSv1/SSLv3 cipher=OTHER); 4, 45 -- Tue, 10 May 2016 09:50:46 -0700 (PDT) 4, 45 -- Message-ID: {57321164.6080006-at-prairienanotech.com} 4, 45 -- Date: Tue, 10 May 2016 11:50:44 -0500 4, 45 -- From: Allen Hall {ajhall-at-prairienanotech.com} 4, 45 -- User-Agent: Postbox 4.0.8 (Macintosh/20151105) 4, 45 -- MIME-Version: 1.0 4, 45 -- To: Microscopy-at-microscopy.com 4, 45 -- Subject: Re: justification of industrial user fee in a university facility 4, 45 -- Content-Type: text/plain; charset=windows-1252 4, 45 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
This issue has been around for a long time. In general, university equipment is there to support the research efforts of their faculty and students. Commercial analytical labs exist which can serve industrial needs. University labs, though, are often tempted to sell their services to industry to make more money. A valid argument is often raised that this is unfair competition to those commercial labs because the (very expensive) university equipment most likely has been purchased with federal grants to support academic research. Some feel this means universities should not do industrial work at all, and others feel it s OK so long as they don t undercut the commercial labs, which could open you up to a lawsuit. Some of the big commercial labs are VERY aggressive about this!
Note (for full disclosure): I am with a small commercial service lab but also work in an academic setting.
Allen J. Hall Prairie Nanotechnology www.prairienanotech.com
==============================Original Headers============================== 4, 45 -- From ajhall-at-prairienanotech.com Tue May 10 11:50:41 2016 4, 45 -- Received: from mail-io0-f178.google.com (mail-io0-f178.google.com [209.85.223.178]) 4, 45 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u4AGofMY014855 4, 45 -- for {Microscopy-at-microscopy.com} ; Tue, 10 May 2016 11:50:41 -0500 4, 45 -- Received: by mail-io0-f178.google.com with SMTP id d62so23241089iof.2 4, 45 -- for {Microscopy-at-microscopy.com} ; Tue, 10 May 2016 09:50:47 -0700 (PDT) 4, 45 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 45 -- d=prairienanotech-com.20150623.gappssmtp.com; s=20150623; 4, 45 -- h=message-id:date:from:user-agent:mime-version:to:subject 4, 45 -- :content-transfer-encoding; 4, 45 -- bh=Plgywb2O1+ImPmxWFmcid2u5uNXP8Sirx3J2XesJPCs=; 4, 45 -- b=t2VgeSn9eeNcFDD2LLgMyflPSDj8b8Xq67qC+mzUypN33zLwJmoTXuHN0x+SjFXYAW 4, 45 -- v4X89t2Jd3S35wojKfAdAAhJI8hiVP46ZjhbCVPLOAzTa4wYOtQGwH23UYRxx/TfALN0 4, 45 -- zTrWblxCxB+O9wlMnlGMp6pLXgVzvMwQfopuvVvHWrDPrfve984uxJ07JYv1EhFO/4XT 4, 45 -- 2MpHY5RRS1SsHmtbF4soLrMBk84IS2xbnRPUvgp/ZmXcRQfCm3w6M0bPTuXEv4S+M6eS 4, 45 -- ACAZzAtMOq2YsAmMCTcDvRyx/+OUOwC9BGA02I0on0USdiPya3uHYZcTB+goErwLxQDZ 4, 45 -- 5ySw== 4, 45 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 45 -- d=1e100.net; s=20130820; 4, 45 -- h=x-gm-message-state:message-id:date:from:user-agent:mime-version:to 4, 45 -- :subject:content-transfer-encoding; 4, 45 -- bh=Plgywb2O1+ImPmxWFmcid2u5uNXP8Sirx3J2XesJPCs=; 4, 45 -- b=QFk4kWfMxbDcQtyn1C+gqxGkLC5+SjI0OA/LMHGxfEPxsP3x4V7K4CYXLufcFUVVLM 4, 45 -- c8akf4kBo8MsjTfzOGAgiFZkDsZBSJvogX2nUSazbTA7WyHvx1wqyeGothvxx0SozVg1 4, 45 -- jL3xVRlXGgGtiopZ649700HJWIg4fj2hU9+dXKRjL3ox8x9uJ3nbfOtXJ15rCrNzOfXs 4, 45 -- 1aJ7jZBrMPbIu3hq2RjvzF71eVnFf7xr/F6DJZH8TFn7bhOLCtuZ1Kkp9jVnQqGr9TMH 4, 45 -- 72aIVum6M85oHgNk9CoQGc3TkDYcqnzq+7LGykHyvwy7cTA9K/PSE/Z59z8fPwcwpTDC 4, 45 -- kCpA== 4, 45 -- X-Gm-Message-State: AOPr4FUkQov8olV3AjXGQ3Oz1CyaYwlE6ETR3r6lvbMv7pLDitpTN4n+LhJS933ONrVvDw== 4, 45 -- X-Received: by 10.107.147.138 with SMTP id v132mr41355964iod.38.1462899046675; 4, 45 -- Tue, 10 May 2016 09:50:46 -0700 (PDT) 4, 45 -- Received: from AllenH.local (c-98-212-86-40.hsd1.il.comcast.net. [98.212.86.40]) 4, 45 -- by smtp.googlemail.com with ESMTPSA id uy5sm1406989igc.4.2016.05.10.09.50.45 4, 45 -- for {Microscopy-at-microscopy.com} 4, 45 -- (version=TLSv1/SSLv3 cipher=OTHER); 4, 45 -- Tue, 10 May 2016 09:50:46 -0700 (PDT) 4, 45 -- Message-ID: {57321164.6080006-at-prairienanotech.com} 4, 45 -- Date: Tue, 10 May 2016 11:50:44 -0500 4, 45 -- From: Allen Hall {ajhall-at-prairienanotech.com} 4, 45 -- User-Agent: Postbox 4.0.8 (Macintosh/20151105) 4, 45 -- MIME-Version: 1.0 4, 45 -- To: Microscopy-at-microscopy.com 4, 45 -- Subject: Re: justification of industrial user fee in a university facility 4, 45 -- Content-Type: text/plain; charset=windows-1252 4, 45 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
Many thanks for all your (so many) answers! The outcome is unambigous -- nail polish. Now up to me to test.
Kind regards, Ondrej
On 4 May 2016 1:01 pm, "Ond ej Koteck " {ondrej.kotecky-at-gmail.com} wrote:
Dear Listers,
Since years we use diamond knives to cut ultrathin sections of polymers at low temperature (around -150 *C). When cut the sections are floating boat/trough integrated on the diamond knife, from where they are collected.
To cut larger sections we started to make and use glass knives on which a plastic boat/trough is glued using a "Cavex Set Up Wax", a dentistry modelling wax, as suggested by the vendor. Unfortunately the boats fall off at low temperature.
Can anyone suggest a solution or a "glue" that would resist to low temperatures?
Many thanks, Ondrej
==============================Original Headers============================== 7, 43 -- From ondrej.kotecky-at-gmail.com Tue May 10 09:45:43 2016 7, 43 -- Received: from mail-io0-f182.google.com (mail-io0-f182.google.com [209.85.223.182]) 7, 43 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u4AEjhPB012444 7, 43 -- for {microscopy-at-microscopy.com} ; Tue, 10 May 2016 09:45:43 -0500 7, 43 -- Received: by mail-io0-f182.google.com with SMTP id d62so18575467iof.2 7, 43 -- for {microscopy-at-microscopy.com} ; Tue, 10 May 2016 07:45:48 -0700 (PDT) 7, 43 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 7, 43 -- d=gmail.com; s=20120113; 7, 43 -- h=mime-version:in-reply-to:references:from:date:message-id:subject:to 7, 43 -- :content-transfer-encoding; 7, 43 -- bh=juGi6TFIPSER3WDp1qp0Oi/1SJybMqR1uPUPBQ95RpA=; 7, 43 -- b=NuhwnPcDJx8SuCFQ79A6qP98FEo1b0fr20ciZtWKnmot3xUS8KR1gjGsAl8KjxH2hS 7, 43 -- FNTOyULlsjNktoBCL9oWgOBcpz69WYXolkKC8TRwLBlE3fzl5aPJCQLB7rrWPvDY4uj5 7, 43 -- dzZCpKLFK79fYObe8lrEz3ZESrHxe6TL7jC2RACL3eRwanw+e4y+6e6W4LsiA+1cVrc0 7, 43 -- zAV25AifBQpMvVFGnalzzBZ1RGtVs9cNrRWMB3A6qnwzVUbz6C/NwchNFNfzTZTJInpP 7, 43 -- X4LRWO7c1+2Pw5wDP1it8hvrLs1dT4dlNP7SuIKx8uoV82U/4GLUjyUv/4XZo/rIQmoO 7, 43 -- q7Aw== 7, 43 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 7, 43 -- d=1e100.net; s=20130820; 7, 43 -- h=x-gm-message-state:mime-version:in-reply-to:references:from:date 7, 43 -- :message-id:subject:to:content-transfer-encoding; 7, 43 -- bh=juGi6TFIPSER3WDp1qp0Oi/1SJybMqR1uPUPBQ95RpA=; 7, 43 -- b=PWISH5GIMOOqvOEBksuPZ5ybnXbsykEShkZDlgusIIBzPUj7GuEzEeHDWkecPafEGe 7, 43 -- ef1rSSjg+VP2APlUAHQ478F3X+1IeUlO1wEG04ywYDdEm795GlfSmlcrE59zAPEo6x2D 7, 43 -- Ud4Dq3zyfdwfC+zI/g625g5dPSTjCVPyE+xIuqxgsdMM82Cw1IflW+OwJb3BOL3tjRzW 7, 43 -- hJgVF/aZ/Gk56h8Jm4oroZ6Ez5FcRJ7wj2VHNELHRnKdhbyImrmF57Qr1GkSVC4VV2Ng 7, 43 -- JS4YYEtlO6OLPR89mZzQc1fKyEsBCoTsKGJ48cRGpUbqdeIV6b30kLUG2+IZ9JwCExMs 7, 43 -- G02w== 7, 43 -- X-Gm-Message-State: AOPr4FWdqDkr2V2jSjFsXg13Sw4hc/iAyHYTiXtu5CglslkrMZmeOuZPruRxIRKG0fpLPdyu0Qh9v/g1mQ2cZg== 7, 43 -- X-Received: by 10.107.163.75 with SMTP id m72mr40810450ioe.79.1462891548277; 7, 43 -- Tue, 10 May 2016 07:45:48 -0700 (PDT) 7, 43 -- MIME-Version: 1.0 7, 43 -- Received: by 10.107.26.68 with HTTP; Tue, 10 May 2016 07:45:29 -0700 (PDT) 7, 43 -- In-Reply-To: {CAJRapMBCgjeRwZ2Vm0WRMcGrEaL83kiF+wh4O-yg_rFhHnL2CQ-at-mail.gmail.com} 7, 43 -- References: {CAJRapMBCgjeRwZ2Vm0WRMcGrEaL83kiF+wh4O-yg_rFhHnL2CQ-at-mail.gmail.com} 7, 43 -- From: =?UTF-8?B?T25kxZllaiBLb3RlY2vDvQ==?= {ondrej.kotecky-at-gmail.com} 7, 43 -- Date: Tue, 10 May 2016 16:45:29 +0200 7, 43 -- Message-ID: {CAJRapMB8sj1Yu1_QHzaAUvBe4xNMujrAudqEim=S6hppyV1_RA-at-mail.gmail.com} 7, 43 -- Subject: Re: Cryomicrotomy using glass knives 7, 43 -- To: microscopy-at-microscopy.com 7, 43 -- Content-Type: text/plain; charset=UTF-8 7, 43 -- Content-Transfer-Encoding: 8bit 7, 43 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u4AEjhPB012444 ==============================End of - Headers==============================
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Email: mjbehr-at-dow.com Name: Michael Behr
Organization: The Dow Chemical Company
Title-Subject: [Filtered] JEOL 2010F
Message: Free JEOL 2010F in working condition, with Gatan Enfina, and Bruker XEDS. You pay for removal from facility in Midland, MI, and shipping to your location. Available June 2016.
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Email: fei.long-at-queensu.ca Name: Fei Long
Organization: Queen's University
Title-Subject: [Filtered] Used 3mm TEM disc puncher, and Tenupol-5 jet
Message: Hi all, I am looking to buy a used TEM 3mm disc puncher. Also a set of the jets with 1mm bore for Tenupol-5 electro-polisher, since the ones I have is broken now.
If anyone has these spare or none used parts, I am interested to purchase them. Thanks,
Fei
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Colleagues, For any film aficianados out there, I have a cameraback and mounting bits, as well as the electronic controller, for a 35 mm camera, that coupled to a Zeiss Axioplan. I was using it up until around 2002 or so, when it was working fine. It has been kept cool and out of dust since then. I think it could probably be adapted to any brand of scope with a bit of hardware tinkering. Free to anyone who wants it (I am willing to pack it carefully for shipment but not pay shipping costs). Thanks, Tobias University of Massachusetts, Biology Department, 611 N Pleasant St, Amherst, MA, 01003, USA
==============================Original Headers============================== 1, 21 -- From baskin-at-bio.umass.edu Mon May 9 16:11:00 2016 1, 21 -- Received: from marlin.bio.umass.edu (marlin.bio.umass.edu [128.119.55.19]) 1, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u49LB0q8023456 1, 21 -- for {microscopy-at-microscopy.com} ; Mon, 9 May 2016 16:11:00 -0500 1, 21 -- Received: from gouzibyte.bio.mor.nsm (beutopia.bio.umass.edu [128.119.55.10]) 1, 21 -- (authenticated bits=0) 1, 21 -- by marlin.bio.umass.edu (8.14.4/8.14.4/Debian-4.1ubuntu1) with ESMTP id u49LB2gc013828 1, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES128-SHA bits=128 verify=NO) 1, 21 -- for {microscopy-at-microscopy.com} ; Mon, 9 May 2016 17:11:02 -0400 1, 21 -- From: Tobias Baskin {baskin-at-bio.umass.edu} 1, 21 -- Subject: 35 mm camera and controller for Zeiss Axioplan to give away 1, 21 -- To: microscnet {microscopy-at-microscopy.com} 1, 21 -- Message-ID: {0ff1830d-6227-1e06-a78e-6a1fbc205134-at-bio.umass.edu} 1, 21 -- Date: Mon, 9 May 2016 17:11:02 -0400 1, 21 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:45.0) 1, 21 -- Gecko/20100101 Thunderbird/45.0 1, 21 -- MIME-Version: 1.0 1, 21 -- Content-Type: text/plain; charset=utf-8; format=flowed 1, 21 -- Content-Transfer-Encoding: 7bit 1, 21 -- X-Greylist: Sender succeeded SMTP AUTH, not delayed by milter-greylist-4.3.9 (marlin.bio.umass.edu [128.119.55.19]); Mon, 09 May 2016 17:11:02 -0400 (EDT) 1, 21 -- X-Scanned-By: MIMEDefang 2.73 ==============================End of - Headers==============================
I agree with Michael's comments, these holes are typical for uninfiltrated dense material. Try letting your specimens sit in uncatalyzed resin at room temperature a bit longer. For Epon I usually do 3h 25%, over night 50% and 2x2h 100% before going into catalyzed resin at 60C 24h. Leaving out the catalyst will also improve the viscosity. You can even heat up the uncatalyzed resin to 37C if you want to push it. You do have quite some crystal damage from freezing, that together with a dry acetone FS will extract a considerable amount of material (see my follow up paper to Walther & Ziegler for more details, http://www.ncbi.nlm.nih.gov/pubmed/18445157 ). Poorly frozen material extracts a lot easier. Here are a few suggestions:
1. If you have adherent cells on sapphire discs, lift them out of the culture dish, touch a filter paper to remove excess medium, dip in hexedecene, then mount for HPF. This ensures you're only freezing the minimal layer of medium with your cells. Freezing adherent cells in pure medium and 200 um deep carriers wil almost certainly fail without cryoprotection as you're essentially tryin to freeze 200 um of pure water. (safety tip: repeated skin exposure to hexadecene will give you contact dermatitis, wear nitrile gloves). 2. If you're working with suspensions, 100 um deep carriers and 20% BSA for cryoprotection works well with most cells and doesn't mess up your FS (in contrast to sugars that won't come out easily during FS). 3. FS in acetone, about 1% osmium tetroxide, about 0.1% uranyl acetate, about 5% water for best contrast. To get there, dissolve OsO4 in acetone, add 1:20 of your 2% aqueous UA and that's it. Some precipitation is normal and doesn't influence the end result, it's a saturated solution particularly at cold temperatures. If it's completely snowed up, try half the amount of 2% UA. 4. I'd advise against prefixation in aldehydes unless your cells are very sensitive (e.g. primary cells) and you can't get them to the HPF within 30ish minutes.
Good luck! Chris
-----Original Message----- X-from: mdelann1-at-jhmi.edu [mailto:mdelann1-at-jhmi.edu] Sent: Monday, May 9, 2016 10:24 AM To: cbuser125-at-gmail.com
Hello Elizabeth and Welcome to the Forum, These "holes" look like un-infiltrated granules. I would see this sometimes with Drosophila eyes and the pigment granules. Some would be infiltrated and others not. The fix was to do longer and more infiltration steps (resin:solvent) and/or switch to a less viscous resin. Were these cells grown on a sapphire disc or is this a suspension that was HPF/AFS? I know mast cells have a large number of secretory granules, but not outside the cells plasma memebrane. Also what is you AFS cocktail? I would also introduce 5% water to your freezing cocktail for better membrane preservation, (Walther, P. & Ziegler, A. Freeze substitution of high-pressure frozen samples: the visibility of biological membranes is improved when the substitution medium contains water. J. Microsc. 208, 3-10). Also what was your cryo-protectant? (what did you HPF the cells in). I believe the HPF images should look a lot better, it looks like there is some freezing artifacts in your cells (look for spider-web like profiles) and your mitochondria are extracted. Were these cells frozen live? If they are clinical you can use the HPF-chemical hybrid technique where you lightly fix the sample in paraformaldehyde only until you can get to the HPF. If you need a good reference for the HPF/AFS techniques, Mary Morphew at Colorado (formerly Kent McDonald's lab) has a very detailed manual online: https://mcdb.colorado.edu/facilities/ems/pdf/mmanual.pdf. Finally all HPF experiments should be accompanied by the standard fixation counterpart for comparison. Perhaps this will give you a clue. Please keep us posted as to you results.
Sincerely, Michael Delannoy
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Monday, May 09, 2016 9:05 AM To: mdelann1-at-jhmi.edu
X-from: elisabeth.pritz-at-medunigraz.at
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Email: elisabeth.pritz-at-medunigraz.at Name: Elisabeth Pritz
Organization: Medical Univeristiy Graz
Title-Subject: [Filtered] TEM: Unknown structures after HPF and AFS in RBL cell line
Message: Hello,
it's my first time using this forum - maybe someone knows these structures and it would be a great help for me to learn more about ultrastructure of biological specimen. We got some unknown structures after high pressure freezing and automated freeze substitution of mast cell line RBL-2H3 (it is not fully representative for mast cell line).
There is a ring of "bubbles" around each cell. However, it seems that the bubbles were filled (please use link below for downloading the pictures). As mentioned before we did high pressure freezing and freeze substitution in a mixture of acetone (waterfree) with osmium tetroxide and uranylacetate. The samples were embedded in TAAB epoxy resin. Unfortunately the membrane contrast is very poor in these samples, though we already fixed that problem. These "bubbles" appear only close to the cells and in nearly every single bubble one could see electron dense remnants.
Is it possible, that the structures are a kind of degranulated vesicles?
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Hi I have very limited experience in TEM so please don't laugh: could they be air associated or originated from the cell, that formed bubbles during the procedure? yorgos
-----Original Message----- X-from: mdelann1-at-jhmi.edu [mailto:mdelann1-at-jhmi.edu] Sent: Monday, May 9, 2016 8:12 PM To: eikonika-at-otenet.gr
Hello Elizabeth and Welcome to the Forum, These "holes" look like un-infiltrated granules. I would see this sometimes with Drosophila eyes and the pigment granules. Some would be infiltrated and others not. The fix was to do longer and more infiltration steps (resin:solvent) and/or switch to a less viscous resin. Were these cells grown on a sapphire disc or is this a suspension that was HPF/AFS? I know mast cells have a large number of secretory granules, but not outside the cells plasma memebrane. Also what is you AFS cocktail? I would also introduce 5% water to your freezing cocktail for better membrane preservation, (Walther, P. & Ziegler, A. Freeze substitution of high-pressure frozen samples: the visibility of biological membranes is improved when the substitution medium contains water. J. Microsc. 208, 3-10). Also what was your cryo-protectant? (what did you HPF the cells in). I believe the HPF images should look a lot better, it looks like there is some freezing artifacts in your cells (look for spider-web like profiles) and your mitochondria are extracted. Were these cells frozen live? If they are clinical you can use the HPF-chemical hybrid technique where you lightly fix the sample in paraformaldehyde only until you can get to the HPF. If you need a good reference for the HPF/AFS techniques, Mary Morphew at Colorado (formerly Kent McDonald's lab) has a very detailed manual online: https://mcdb.colorado.edu/facilities/ems/pdf/mmanual.pdf. Finally all HPF experiments should be accompanied by the standard fixation counterpart for comparison. Perhaps this will give you a clue. Please keep us posted as to you results.
Sincerely, Michael Delannoy
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Monday, May 09, 2016 9:05 AM To: mdelann1-at-jhmi.edu
X-from: elisabeth.pritz-at-medunigraz.at
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Email: elisabeth.pritz-at-medunigraz.at Name: Elisabeth Pritz
Organization: Medical Univeristiy Graz
Title-Subject: [Filtered] TEM: Unknown structures after HPF and AFS in RBL cell line
Message: Hello,
it's my first time using this forum - maybe someone knows these structures and it would be a great help for me to learn more about ultrastructure of biological specimen. We got some unknown structures after high pressure freezing and automated freeze substitution of mast cell line RBL-2H3 (it is not fully representative for mast cell line).
There is a ring of "bubbles" around each cell. However, it seems that the bubbles were filled (please use link below for downloading the pictures). As mentioned before we did high pressure freezing and freeze substitution in a mixture of acetone (waterfree) with osmium tetroxide and uranylacetate. The samples were embedded in TAAB epoxy resin. Unfortunately the membrane contrast is very poor in these samples, though we already fixed that problem. These "bubbles" appear only close to the cells and in nearly every single bubble one could see electron dense remnants.
Is it possible, that the structures are a kind of degranulated vesicles?
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Hello Elizabeth and Welcome to the Forum, These "holes" look like un-infiltrated granules. I would see this sometimes with Drosophila eyes and the pigment granules. Some would be infiltrated and others not. The fix was to do longer and more infiltration steps (resin:solvent) and/or switch to a less viscous resin. Were these cells grown on a sapphire disc or is this a suspension that was HPF/AFS? I know mast cells have a large number of secretory granules, but not outside the cells plasma memebrane. Also what is you AFS cocktail? I would also introduce 5% water to your freezing cocktail for better membrane preservation, (Walther, P. & Ziegler, A. Freeze substitution of high-pressure frozen samples: the visibility of biological membranes is improved when the substitution medium contains water. J. Microsc. 208, 3-10). Also what was your cryo-protectant? (what did you HPF the cells in). I believe the HPF images should look a lot better, it looks like there is some freezing artifacts in your cells (look for spider-web like profiles) and your mitochondria are extracted. Were these cells frozen live? If they are clinical you can use the HPF-chemical hybrid technique where you lightly fix the sample in paraformaldehyde only until you can get to the HPF. If you need a good reference for the HPF/AFS techniques, Mary Morphew at Colorado (formerly Kent McDonald's lab) has a very detailed manual online: https://mcdb.colorado.edu/facilities/ems/pdf/mmanual.pdf. Finally all HPF experiments should be accompanied by the standard fixation counterpart for comparison. Perhaps this will give you a clue. Please keep us posted as to you results.
Sincerely, Michael Delannoy
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Monday, May 09, 2016 9:05 AM To: mdelann1-at-jhmi.edu
X-from: elisabeth.pritz-at-medunigraz.at
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Email: elisabeth.pritz-at-medunigraz.at Name: Elisabeth Pritz
Organization: Medical Univeristiy Graz
Title-Subject: [Filtered] TEM: Unknown structures after HPF and AFS in RBL cell line
Message: Hello,
it's my first time using this forum - maybe someone knows these structures and it would be a great help for me to learn more about ultrastructure of biological specimen. We got some unknown structures after high pressure freezing and automated freeze substitution of mast cell line RBL-2H3 (it is not fully representative for mast cell line).
There is a ring of "bubbles" around each cell. However, it seems that the bubbles were filled (please use link below for downloading the pictures). As mentioned before we did high pressure freezing and freeze substitution in a mixture of acetone (waterfree) with osmium tetroxide and uranylacetate. The samples were embedded in TAAB epoxy resin. Unfortunately the membrane contrast is very poor in these samples, though we already fixed that problem. These "bubbles" appear only close to the cells and in nearly every single bubble one could see electron dense remnants.
Is it possible, that the structures are a kind of degranulated vesicles?
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To recap: I placed a request for opinions on photo printers for micrographs, now that HP seems to be out of the game. I got a few responses (surprisingly few, given past interest in this topic), but those that expressed a preference all liked the Canon P600. Which does look good on paper (it's Monday morning, so that's the best pun I've got).
Thanks to all who responded.
Phil -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 3, 30 -- From oshel1pe-at-cmich.edu Mon May 9 08:32:00 2016 3, 30 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 3, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u49DW0KG025044 3, 30 -- for {microscopy-at-microscopy.com} ; Mon, 9 May 2016 08:32:00 -0500 3, 30 -- Received: from cas.cmich.edu ([141.209.15.90]) 3, 30 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id u49DVxZv025935 3, 30 -- for {microscopy-at-microscopy.com} ; Mon, 9 May 2016 09:32:03 -0400 3, 30 -- Received: from cas1.central.cmich.local (2002:8dd1:f28::8dd1:f28) by 3, 30 -- CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) with Microsoft SMTP Server 3, 30 -- (TLS) id 14.3.248.2; Mon, 9 May 2016 09:32:00 -0400 3, 30 -- Received: from bio-br024c-mac01.local (141.209.2.100) by mail.cmich.edu 3, 30 -- (141.209.15.40) with Microsoft SMTP Server (TLS) id 14.3.248.2; Mon, 9 May 3, 30 -- 2016 09:32:00 -0400 3, 30 -- Message-ID: {57309150.4030202-at-cmich.edu} 3, 30 -- Date: Mon, 9 May 2016 09:32:00 -0400 3, 30 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 3, 30 -- Reply-To: {oshel1pe-at-cmich.edu} 3, 30 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 3, 30 -- MIME-Version: 1.0 3, 30 -- To: micro {microscopy-at-microscopy.com} 3, 30 -- Subject: New photo printer for micrographs, for those interested 3, 30 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 3, 30 -- Content-Transfer-Encoding: 7bit 3, 30 -- X-Originating-IP: [141.209.2.100] 3, 30 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 3, 30 -- X-Spam-Score: -0.30 () [Hold at 6.00] L_CTCMUM,L_TMAILSRV,RDNS_NONE,SPF(softfail:0),Bayes(0.0001:-0.5) 3, 30 -- X-CanIt-Geo: ip=141.209.15.90; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6 3, 30 -- X-CanItPRO-Stream: default 3, 30 -- X-Canit-Stats-ID: 02QQdw3IV - 77565d9be353 - 20160509 3, 30 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
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Email: elisabeth.pritz-at-medunigraz.at Name: Elisabeth Pritz
Organization: Medical Univeristiy Graz
Title-Subject: [Filtered] TEM: Unknown structures after HPF and AFS in RBL cell line
Message: Hello,
it's my first time using this forum - maybe someone knows these structures and it would be a great help for me to learn more about ultrastructure of biological specimen. We got some unknown structures after high pressure freezing and automated freeze substitution of mast cell line RBL-2H3 (it is not fully representative for mast cell line).
There is a ring of "bubbles" around each cell. However, it seems that the bubbles were filled (please use link below for downloading the pictures). As mentioned before we did high pressure freezing and freeze substitution in a mixture of acetone (waterfree) with osmium tetroxide and uranylacetate. The samples were embedded in TAAB epoxy resin. Unfortunately the membrane contrast is very poor in these samples, though we already fixed that problem. These "bubbles" appear only close to the cells and in nearly every single bubble one could see electron dense remnants.
Is it possible, that the structures are a kind of degranulated vesicles?
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Email: scottserata-at-gmail.com Name: Scott Serata
Organization: California Academy of Sciences/JEOL
Title-Subject: [Filtered] detailed instructions for LEO (Zeiss) 1450VP SEM
Message: Several people have asked me to post this on the listserve. I have written detailed instructions for the LEO/Zeiss 1450VP SEM. I was the engineer at the California Academy of Sciences responsible for training users and fixing this machine for 15 years, until I finally got rid of it last year. (I now work for JEOL) Our machine did not have any analytical equipment (X-ray EDS/WDS) on it, just the SE, VPSE and BSD detectors. If you have a LEO 1450 VP SEM this maybe useful. It is in PDF format and about 13 MB.
Scott Serata
click on this link to get it from my Google drive https://drive.google.com/open?id=0B2-Pe5FzqYv2TWdUNXNLQTZLQmM
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Title-Subject: [Filtered] Free Megaview II CCD System for TEM
Message: TSS has one complete Megaview II CCD Side-mount Camera for Philips/FEI Series TEM s that needs to find a home. Functional and complete side-mount CCD camera solution with computer and cable set. Pictures may be seen of the camera/flange here:
https://tss.exavault.com/share/view/bemn-fgdqg1k7
Will carefully prepare for shipment and with your shipping label and send to you anywhere in the world.
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Like you say, we take a look at local rates for similar services and price = higher so as to not undercut the local business.
If anyone gives me a hard time about rates I compare it to what they would = pay a professional photographer to take high school senior pictures or wedd= ing photos. EM Lab rates start to look entirely reasonable, especially give= n our "camera" is on the order of hundreds of thousands of dollars instead = of hundreds of dollars.
Kind regards,
William Stonewall Monroe University of Alabama -at- Birmingham SEM Lab http://www.uab.edu/scanningelectronmicroscopy
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Title-Subject: [Filtered] justification of industrial user fee in a uiversity facility Message: Dear listers, How do your facilities, especially for the electron microscopes, justify the industrial user rate? Do you consider the local analytical companies rate so that yours won't undercut theirs? I looked up the SEM/TEM rate across the universties nationwide and found it varies a lot, ranging from less $100/h to $300/h for industrial users. Thanks.
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==============================Original Headers============================== 10, 52 -- From microscopy.listserver-at-gmail.com Thu May 5 20:16:25 2016 10, 52 -- Received: from mail-qg0-f44.google.com (mail-qg0-f44.google.com [209.85.192.44]) 10, 52 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u461GPbK024302 10, 52 -- for {microscopy-at-microscopy.com} ; Thu, 5 May 2016 20:16:25 -0500 10, 52 -- Received: by mail-qg0-f44.google.com with SMTP id f92so49409392qgf.0 10, 52 -- for {microscopy-at-microscopy.com} ; Thu, 05 May 2016 18:19:18 -0700 (PDT) 10, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 10, 52 -- d=gmail.com; s=20120113; 10, 52 -- h=from:subject:references:to:reply-to:message-id:date:user-agent 10, 52 -- :mime-version:in-reply-to:content-transfer-encoding; 10, 52 -- bh=SDeVEu/IJUmDBKJGbWPt3Vw9+AGmU17Cu4hF+DptxCE=; 10, 52 -- b=fQ8BJ3dKsgXkISNj1F7oymt9I/vYRclrSDoCezWviCZ6tk/UDlURrZEGyvMtvqibX8 10, 52 -- QMAL+Hor5mIbbwwnYyNEGFWl35xXCUO6tFujxoZm2ixEUpDR1ekxnyBrVYK+tAJYKdWi 10, 52 -- oVsnu1yHtpXCoY7P8GWlV9wCDpm32h0zyAYMDti6vh3Cd8/InnxH+haX1ZiLZr0OPH9d 10, 52 -- Z70xUjUGk7TaXuQUWvXEUidTGIUkoZirinx0kbRZnhKozBQ7GxEgaKZatm68H+s6iXTt 10, 52 -- 2RrcoKOmvormf1RQ4Lza3wKH5APsH+eZ0XMgmQH9fL8AEckZkGlua2RDY6oxZy0pFSC2 10, 52 -- mrng== 10, 52 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 10, 52 -- d=1e100.net; s=20130820; 10, 52 -- h=x-gm-message-state:from:subject:references:to:reply-to:message-id 10, 52 -- :date:user-agent:mime-version:in-reply-to:content-transfer-encoding; 10, 52 -- bh=SDeVEu/IJUmDBKJGbWPt3Vw9+AGmU17Cu4hF+DptxCE=; 10, 52 -- b=BLJLq3IuTYAtaqeYF6A2mLk81ZByE6eFW4Qe3LjPqPu5VqWL53LHy2LCtuJwhQY1Ja 10, 52 -- VvC5WUdG4eysRt5VJSIW+EkoBvpD3lrtn9hd09TPdN1FshILQyAFsuoaDTWr0A+a/CNO 10, 52 -- 5FG0mGIjaliyBr/8cHhVBjOQJlSyEYlN8jAJCGvbSykdYawcy+Yrtn4LDuYofenkU6b9 10, 52 -- GiatPekKT9AwWkBAc57eennrQRkh5oJDwgyW+k9OLs6HQTkZ/jFFmh3Px7WHP6PhMwZe 10, 52 -- lOoR1N3FIXG8AXUBs0V0nOpEqxuERu+p3QU6XKggQZjziqTa4GHKpJ362C5CqjTc6/fd 10, 52 -- QE2g== 10, 52 -- X-Gm-Message-State: AOPr4FUnms36E2y6Op368tFk6PCdzGcWmv7QzVQlHlvu5dwyafpGmlUn5tb+3V+M5xb+zQ== 10, 52 -- X-Received: by 10.140.22.203 with SMTP id 69mr2840140qgn.62.1462497557768; 10, 52 -- Thu, 05 May 2016 18:19:17 -0700 (PDT) 10, 52 -- Received: from anlvpn001.nst.anl.gov ([130.202.235.1]) 10, 52 -- by smtp.googlemail.com with ESMTPSA id c33sm4694733qga.39.2016.05.05.18.19.16 10, 52 -- for {microscopy-at-microscopy.com} 10, 52 -- (version=TLSv1/SSLv3 cipher=OTHER); 10, 52 -- Thu, 05 May 2016 18:19:17 -0700 (PDT) 10, 52 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 10, 52 -- X-Google-Original-From: MicroscopyListserver-NoReply 10, 52 -- {microscopylistserver-noreply-at-microscopy.com} 10, 52 -- Subject: viaWWW:justification of industrial user fee in a uiversity facility 10, 52 -- References: {201605052145.u45Ljrio019548-at-ns.microscopy.com} 10, 52 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 10, 52 -- Reply-To: microscopylistserver-noreply-at-microscopy.com 10, 52 -- X-Forwarded-Message-Id: {201605052145.u45Ljrio019548-at-ns.microscopy.com} 10, 52 -- Message-ID: {4bb26c2f-1c69-d2f5-6069-af4d0aefb8dd-at-microscopy.com} 10, 52 -- Date: Thu, 5 May 2016 20:19:16 -0500 10, 52 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:45.0) 10, 52 -- Gecko/20100101 Thunderbird/45.0 10, 52 -- MIME-Version: 1.0 10, 52 -- In-Reply-To: {201605052145.u45Ljrio019548-at-ns.microscopy.com} 10, 52 -- Content-Type: text/plain; charset=windows-1252; format=flowed 10, 52 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
If you are not fussy or fancy just drive some sharpened 1/8" diameter stainless steel rods through a clean rubber stopper. Will work OK through the HV range (10^-6 Torr) , but probably not into the UHV range.
==============================Original Headers============================== 1, 42 -- From bigelow-at-umich.edu Thu May 5 13:47:37 2016 1, 42 -- Received: from mail-wm0-f46.google.com (mail-wm0-f46.google.com [74.125.82.46]) 1, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u45Ilbls023188 1, 42 -- for {microscopy-at-microscopy.com} ; Thu, 5 May 2016 13:47:37 -0500 1, 42 -- Received: by mail-wm0-f46.google.com with SMTP id a17so42508274wme.0 1, 42 -- for {microscopy-at-microscopy.com} ; Thu, 05 May 2016 11:50:28 -0700 (PDT) 1, 42 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 1, 42 -- d=umich.edu; s=google; 1, 42 -- h=message-id:date:from:user-agent:mime-version:to:subject 1, 42 -- :content-transfer-encoding; 1, 42 -- bh=3bbbWfX1sDpnKVTCO89Hjj6HEN6J/n3Vy9oX4WnYMco=; 1, 42 -- b=PN2s3z03LUl1lZCMHVkAk6dMHP+JXoAvKck86xJqHqeaeCYek/hpDUFtu4flG8dyoh 1, 42 -- BwRgQkEtLZPAF9TTyNwVxbjI15hWNxMuE8/YnFQ7McADcY34/OsFLzYcLT3StJTlUE6U 1, 42 -- V6k4NJoCHQ1wxN87xkNFHw9FHJcyI3Lju0TJc= 1, 42 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 1, 42 -- d=1e100.net; s=20130820; 1, 42 -- h=x-gm-message-state:message-id:date:from:user-agent:mime-version:to 1, 42 -- :subject:content-transfer-encoding; 1, 42 -- bh=3bbbWfX1sDpnKVTCO89Hjj6HEN6J/n3Vy9oX4WnYMco=; 1, 42 -- b=JqeRpKe/fqzx8vZr+8gHGkt49U/a/Eo/oFeKCujXMVUUI+A97c5wTFBGt8/1ib4Bkt 1, 42 -- wlIYewSTHCTzivwrzGhSbwcqO2P52qREVVx1rqEzE4ytHh3UKE/RKQqoFa1HlOqo/ofJ 1, 42 -- zLllgfb3z38RieqYFUPVTInWguY9gjusF2NIxNxP6ADgkJ3Frr4fKSCNiOWxjdznnRgM 1, 42 -- x41jLxNcbfQFq0F6TBQg4KTS6d96Czotn7Lu9Llw6VwWXqHIF4b+JCYPrSGteiMtUCKx 1, 42 -- jbFCBjICyucgqPeAgm9sbTCIBJ69Q7t8WXBQJE75A97toiqercgV4qlr5Lqqlz1idhyb 1, 42 -- pB4w== 1, 42 -- X-Gm-Message-State: AOPr4FUd9PlZ9IePDj8akeu3j5TZchle2s1JpKnDShGEsCOLRjQ503pse6ojUlPCeoI6I0JL 1, 42 -- X-Received: by 10.194.139.104 with SMTP id qx8mr15321433wjb.14.1462474228130; 1, 42 -- Thu, 05 May 2016 11:50:28 -0700 (PDT) 1, 42 -- Received: from Wilbur-Bigelows-G4.local ([2602:306:3b93:aba0:230:65ff:fe6b:155c]) 1, 42 -- by smtp.gmail.com with ESMTPSA id i194sm4492750wmf.6.2016.05.05.11.50.27 1, 42 -- for {microscopy-at-microscopy.com} 1, 42 -- (version=TLS1 cipher=ECDHE-RSA-AES128-SHA bits=128/128); 1, 42 -- Thu, 05 May 2016 11:50:27 -0700 (PDT) 1, 42 -- Message-ID: {572B95F1.1060904-at-umich.edu} 1, 42 -- Date: Thu, 05 May 2016 14:50:25 -0400 1, 42 -- From: Wil Bigelow {bigelow-at-umich.edu} 1, 42 -- User-Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X 10.4; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 1, 42 -- MIME-Version: 1.0 1, 42 -- To: microscopy-at-microscopy.com 1, 42 -- Subject: [Microscopy]Electrical feedthroughs 1, 42 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 1, 42 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
My friend recently made a feedthrough using stiff piano wire, a rubber stopper from home depot, and super glue (I think, or maybe it was torr-seal type epoxy). He used a DB9 port to mark the pin locations, then drilled pilot holes (which I don't think were fully drilled through the rubber) and I believe then he pounded the wire through the remainder of the rubber. This stopper was jammed into a tapered hole in a blank/unused blocking plate that came with his machine. It holds working pressure as good as before the feedthrough was installed. If you want more info, I can probably pass you his direct email to ask for more details.
On Thu, May 5, 2016 at 5:39 AM, {microscopy.listserver-at-gmail.com} wrote:
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Email: wambrose-at-unc.edu Name: Wallace Ambrose
Organization: CHANL UNC
Title-Subject: [Filtered] electrical pass through for FEI ESEM Message: Hello, I would like to do voltage contrast and active EBIC on our FEI Quanta ESEM and Helios FIB. I am interested in knowing what folks have done to make up pass through plates (and what connectors (BNC, DB9 or DB25) would be the most useful for this for the 4 or 2 3/8 inch ports. thanks Wallace Login Host: 152.19.204.249 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
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Email: duraine-at-bcm.edu Name: Lita Duraine
Organization: Howard Hughes Medical Institute
Title-Subject: [Microscopy] RE: Cryomicrotomy using glass knives
Message: Hi Ondrej,
At Delta microscopy school some of us used 3M Silver Polyester Tape, Nonconductive, 850 from Ted Pella to make boats for glass knives. I think the tape is rated for -150 degrees C. Ted Pella also carries other type tapes that may be used for making boats with lower temp ranges. You might check.
Regards,
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Email: peter.eschbach-at-oregonstate.edu Name: Peter Eschbach
Organization: Oregon State University
Title-Subject: [Filtered] Importing .txt files into Digital Micrograph for EELS
Message: Hi:
We do not have a digiscan box to control our Titan from Digital Micrograph (DM). So, for now we are collecting simultaneous EELS and EDX linescans in FEI's TIA software. I can do rudimentary analysis in TIA on all the EELS data but would like to import several EELS spectra from my linescan into DM. I am able to save the TIA acquired EELS data into .txt files with two nice columns, Energy and Intensity. But, does anybody know of a script to convert the .txt files into DM3? We looked on David Mitchell's site, but nothing jumped out. Thanks, Pete Eschbach Oregon State University EM Facility 541 737 5645
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Email: wambrose-at-unc.edu Name: Wallace Ambrose
Organization: CHANL UNC
Title-Subject: [Filtered] electrical pass through for FEI ESEM Message: Hello, I would like to do voltage contrast and active EBIC on our FEI Quanta ESEM and Helios FIB. I am interested in knowing what folks have done to make up pass through plates (and what connectors (BNC, DB9 or DB25) would be the most useful for this for the 4 or 2 3/8 inch ports. thanks Wallace Login Host: 152.19.204.249 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
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Don: A pumping speed of 1 cfm is a pretty high rate of flow (28 liters/min) and typical of values for small rotary vane pumps. For this type of pump the speed is usually rated as the volume of gas moved through the pump when both the inlet and outlet ports are at atmospheric presure. I wonder if you would really need a pumping speed of 1 cfm for a device that merely holds a wafer in place. The question is, what is the actual volume that needs to be evacuated, and how much of a hurry are you in to get the wafer held firmly in place. If you just need to evacuate a tube of modest diameter and modest length leading to a small chamber under the wafer you certainly don't need a speed of 1 cfm to do the job, even if the system leaks pretty badly.
Wil
==============================Original Headers============================== 2, 42 -- From bigelow-at-umich.edu Wed May 4 19:24:32 2016 2, 42 -- Received: from mail-wm0-f44.google.com (mail-wm0-f44.google.com [74.125.82.44]) 2, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u450OVBO002754 2, 42 -- for {microscopy-at-microscopy.com} ; Wed, 4 May 2016 19:24:32 -0500 2, 42 -- Received: by mail-wm0-f44.google.com with SMTP id a17so2635129wme.0 2, 42 -- for {microscopy-at-microscopy.com} ; Wed, 04 May 2016 17:27:20 -0700 (PDT) 2, 42 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 2, 42 -- d=umich.edu; s=google; 2, 42 -- h=message-id:date:from:user-agent:mime-version:to:subject 2, 42 -- :content-transfer-encoding; 2, 42 -- bh=hq58s9xkmj+ZbM9fQ2wwIYVZX1qv8u2pIM2DIldDGuc=; 2, 42 -- b=IcuIgLDQ5CQ/kyLNfMDtVoNXfzUT37OybdkPqO2V5iYhAYD7FYCiYV7v68vKxClQ9H 2, 42 -- OQ3G61zMM9K6Z+IwnIDM0/5rHL0vM9Yp9Nd8n8Ht5VWfAN99YrsgMKeZ4eLHpBk5OBHr 2, 42 -- V2KYYK9NiN9VK6AeIhTbKYk/WxED89i2fHy8I= 2, 42 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 2, 42 -- d=1e100.net; s=20130820; 2, 42 -- h=x-gm-message-state:message-id:date:from:user-agent:mime-version:to 2, 42 -- :subject:content-transfer-encoding; 2, 42 -- bh=hq58s9xkmj+ZbM9fQ2wwIYVZX1qv8u2pIM2DIldDGuc=; 2, 42 -- b=NKBQMOmf+dccqKPbu/tmIUz3H/ZLU+qKGpmzDtVD43h9LQfCiHKC2a1NzN0z2zU2T9 2, 42 -- LejstTbCX51qWliRLGYVsXyyKRIVvfmQrHwEOn97pDfzd67e+iEZJS9o8j3j2wiQkHh/ 2, 42 -- +Lw4WSeQAb3XrkZzvngaGjABibh9LBUbMT92dXzw1IwC5Qog3hK7A/sEPyAUGuwgu3g5 2, 42 -- U3L2Uj7MlUuB+/pFB1TEUDCbh5Ka3QW7qYSwLpseMdUmQ2nTysDjQW6VYMPH283V81IZ 2, 42 -- /knMFuE6SqYNyuFTS9AcV3Ej2tEhcQoIz3Ja1cLjGHQcs6zzV8SjJh5fkt85iWzYtgtD 2, 42 -- b3Ng== 2, 42 -- X-Gm-Message-State: AOPr4FU8hFs4U2inG+8+tUz9dN4gs81y+zjx7L9vV6Jdxkl82deva3Eo3VjgTH5dm+69d1KA 2, 42 -- X-Received: by 10.194.187.236 with SMTP id fv12mr11238503wjc.96.1462408039588; 2, 42 -- Wed, 04 May 2016 17:27:19 -0700 (PDT) 2, 42 -- Received: from Wilbur-Bigelows-G4.local ([2602:306:3b93:aba0:230:65ff:fe6b:155c]) 2, 42 -- by smtp.gmail.com with ESMTPSA id on2sm6872092wjc.32.2016.05.04.17.27.18 2, 42 -- for {microscopy-at-microscopy.com} 2, 42 -- (version=TLS1 cipher=ECDHE-RSA-AES128-SHA bits=128/128); 2, 42 -- Wed, 04 May 2016 17:27:19 -0700 (PDT) 2, 42 -- Message-ID: {572A9365.600-at-umich.edu} 2, 42 -- Date: Wed, 04 May 2016 20:27:17 -0400 2, 42 -- From: Wil Bigelow {bigelow-at-umich.edu} 2, 42 -- User-Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X 10.4; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 2, 42 -- MIME-Version: 1.0 2, 42 -- To: microscopy-at-microscopy.com 2, 42 -- Subject: [Microscopy]Pump speed for wafer holder 2, 42 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 2, 42 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Dear Fred, Thanks very much for this explanation. Don
----- Original Message ----- From: Frederick Schamber To: Don Chernoff at ASM Sent: Wednesday, May 04, 2016 5:31 PM Subject: [a] Re: how are vacuum pumps rated?
Three things: (1) Vacuum pump ratings (pumping speed) always apply to the inlet (evacuated).
(2) If they say cfm (or lps) it is 'pumping speed' and that is independent of either internal or external pressure. Think of having a 'demon' collecting one cubic foot of gas in a (previously evacuated) canister while inside the chamber. The demon then seals the canister and removes it from the chamber. To repeat the cycle he shrinks the canister to zero volume ( thereby expelling the collected gas) and then goes back into the chamber where he expands it to 1 cubic foot again. If he does this once a minute, that's a 1 cfm pumping speed. It's easiest to see that's what a piston pump does but it's the same principle for rotary vane (and can be generalized for a turbo). If you think of the 'demon' model it's clear that the volume per second is a function only of the canister size and repetition rate, not the pressure (either internal nor external. That's an idealization for an actual pump of course due to things like leakage, turbulent flow, and lots of other messy things. But in principle, volume pumping speed is constant.
(3) Mass flow rate is density times pumping speed and would be something like grams per second. Since the density of the gas decreases, the mass flow rate also decreases with improving vacuum. A pump's 'throughout' is defined as inlet pressure x pumping speed and is proportional to mass flow rate.
Actually, mass flow rate (and throughout) can be defined and is invariant anywhere in the system, so long as volume and density are defined at the same place. So you can use the constancy of throughput to calculate the volume of gas exhausted for a given pumping speed and inlet pressure.
Any help?
Fred
Sent from my iPhone
On May 4, 2016, at 4:14 PM, Don Chernoff at ASM {donc-at-asmicro.com} wrote:
Dear Fred, Thanks for your reply. I you're pointing in the right direction. My question is a practical one: if someone says "you need a flow rate of 1 cfm" does that mean 1 cfm measured at P = 1 atmosphere? Don ----- Original Message ----- From: Frederick Schamber To: donc-at-asmicro.com Sent: Wednesday, May 04, 2016 3:13 PM
Dear Fred, Thanks for your reply. I you're pointing in the right direction. My question is a practical one: if someone says "you need a flow rate of 1 cfm" does that mean 1 cfm measured at P = 1 atmosphere? Don ----- Original Message ----- From: Frederick Schamber To: donc-at-asmicro.com Sent: Wednesday, May 04, 2016 3:13 PM Subject: [a] Re: [Microscopy] how are vacuum pumps rated?
Don,
Maybe I misunderstand your question, but I was long puzzled by how mechanical vacuum pumps are rated. Finally I realized that the mechanism of a vacuum pump will capture and remove a constant volume of gas regardless of internal pressure. That is, regardless of that internal pressure, the volume swept out remains the same. Clearly, that means that the volume of gas exhausted decreases proportionately as the internal pressure declines. This is why we use 'mass flow' when we want to quantify the mass of material pumped.
So if you are looking at volume of gas removed from the chamber the pumping speed is (essentially) constant. But if you are looking at volume or mass of gas exhausted, the pumping speed varies proportionately to internal pressure.
Does this help?
Fred Schamber (retired, but still interested)
On Wed, May 4, 2016 at 2:27 PM, {donc-at-asmicro.com} wrote:
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Dear All, I want to thank the many people who responded to my initial post about low vacuum pump.
In reviewing vacuum pump specifications, I note that the flow rate chart shows a high rate at atmospheric pressure with a rapid decrease in rate as the input pressure decreases. When this type of pump is specified, is there general agreement that the flow rate is specified for free air (at 1 atmosphere pressure)? regards, Don ============================================= Don Chernoff, Ph.D., President Advanced Surface Microscopy, Inc.; 3250 N. Post Rd., Suite 120; Indianapolis IN 46226 USA E-Mail: donc-at-asmicro.com www.asmicro.com Voice: +1-317-895-5630 Toll free: +1-800-374-8557 (in USA & Canada) Fax: +1-317-895-5652 [business activities since 1990: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.] ============================================= ----- Original Message ----- From: Don Chernoff at ASM To: Microscopy List Sent: Sunday, May 01, 2016 7:01 PM Subject: low vacuum pump
I'm working with a wafer inspection system that uses vacuum to clamp the wafer. The manufacturer's specifications call for a modest vacuum:
low vacuum: 150 torr (-24" Hg)
flow rate: { 1 cfm (28 l/m)
Actual flow will be intermittent.
What type of pump would be suitable for this application?
I welcome suggestions for specific models and suggestions for how to configure the pump.
regards, Don ============================================= Don Chernoff, Ph.D., President Advanced Surface Microscopy, Inc.; 3250 N. Post Rd., Suite 120; Indianapolis IN 46226 USA E-Mail: donc-at-asmicro.com www.asmicro.com Voice: +1-317-895-5630 Toll free: +1-800-374-8557 (in USA & Canada) Fax: +1-317-895-5652 [business activities since 1990: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.] =============================================
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Dear All, I want to thank the many people who responded to my initial post about low vacuum pump.
In reviewing vacuum pump specifications, I note that the flow rate chart shows a high rate at atmospheric pressure with a rapid decrease in rate as the input pressure decreases. When this type of pump is specified, is there general agreement that the flow rate is specified for free air (at 1 atmosphere pressure)? regards, Don ============================================= Don Chernoff, Ph.D., President Advanced Surface Microscopy, Inc.; 3250 N. Post Rd., Suite 120; Indianapolis IN 46226 USA E-Mail: donc-at-asmicro.com www.asmicro.com Voice: +1-317-895-5630 Toll free: +1-800-374-8557 (in USA & Canada) Fax: +1-317-895-5652 [business activities since 1990: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.] ============================================= ----- Original Message ----- From: Don Chernoff at ASM To: Microscopy List Sent: Sunday, May 01, 2016 7:01 PM Subject: low vacuum pump
I'm working with a wafer inspection system that uses vacuum to clamp the wafer. The manufacturer's specifications call for a modest vacuum:
low vacuum: 150 torr (-24" Hg)
flow rate: { 1 cfm (28 l/m)
Actual flow will be intermittent.
What type of pump would be suitable for this application?
I welcome suggestions for specific models and suggestions for how to configure the pump.
regards, Don ============================================= Don Chernoff, Ph.D., President Advanced Surface Microscopy, Inc.; 3250 N. Post Rd., Suite 120; Indianapolis IN 46226 USA E-Mail: donc-at-asmicro.com www.asmicro.com Voice: +1-317-895-5630 Toll free: +1-800-374-8557 (in USA & Canada) Fax: +1-317-895-5652 [business activities since 1990: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.] =============================================
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Since years we use diamond knives to cut ultrathin sections of polymers at low temperature (around -150 *C). When cut the sections are floating boat/trough integrated on the diamond knife, from where they are collected.
To cut larger sections we started to make and use glass knives on which a plastic boat/trough is glued using a "Cavex Set Up Wax", a dentistry modelling wax, as suggested by the vendor. Unfortunately the boats fall off at low temperature.
Can anyone suggest a solution or a "glue" that would resist to low temperatures?
Many thanks, Ondrej
==============================Original Headers============================== 6, 41 -- From ondrej.kotecky-at-gmail.com Wed May 4 05:59:00 2016 6, 41 -- Received: from mail-ig0-f180.google.com (mail-ig0-f180.google.com [209.85.213.180]) 6, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u44Ax0kR020028 6, 41 -- for {microscopy-at-microscopy.com} ; Wed, 4 May 2016 05:59:00 -0500 6, 41 -- Received: by mail-ig0-f180.google.com with SMTP id bi2so143580313igb.0 6, 41 -- for {microscopy-at-microscopy.com} ; Wed, 04 May 2016 04:01:47 -0700 (PDT) 6, 41 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 41 -- d=gmail.com; s=20120113; 6, 41 -- h=mime-version:from:date:message-id:subject:to 6, 41 -- :content-transfer-encoding; 6, 41 -- bh=yHiwbV2Nl3yhgTFlabuEWXh+sc1xNAqG4DWxo1ckEHM=; 6, 41 -- b=fR31egbWeNkAcnAQx2R/TkMkG5bPvJ8keX1dwCVPtJ5rpp240JFRXMy4xaSdgJYtYI 6, 41 -- /JQ9PHNJiwGhi7/UR0jxgz0dAl08EWogwWdDtXnmfTfFWh6lxvYdK/TS0OdlEXlR6g91 6, 41 -- MjYphFDqaDPs9d2NIyitBlcgJlNxhGa/i7U9vEfHCArHCEQDubkZTNFij8uc+hkpPQ9C 6, 41 -- Id9tq+BAvvYpxYCaXovETpq1RvqvxEQd9VFHZLJ3CilDn3IQ6x/wigSFR9DgAtwTu66T 6, 41 -- Vo5+q+iWcDllCAZwSfx7QiM3BH2w6sGEbNxdoBymXx7Q0mZGPtUkWTggzyXFpPgmXDGU 6, 41 -- qw3w== 6, 41 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 41 -- d=1e100.net; s=20130820; 6, 41 -- h=x-gm-message-state:mime-version:from:date:message-id:subject:to 6, 41 -- :content-transfer-encoding; 6, 41 -- bh=yHiwbV2Nl3yhgTFlabuEWXh+sc1xNAqG4DWxo1ckEHM=; 6, 41 -- b=K09QTjkxG60A/LJd7Ol014juUHvA102QM1V+5tlBvF7d9z/O83HcYFk12K/UE67iZA 6, 41 -- e+9i+W21EnWxm9mWIGCVsQcgk45kKEP3rnZH8EIBIIuCajkqPIYoIrc4RmP7WoXZX36i 6, 41 -- Kkjz4G4E4MBBEAHM6huqv652y5jk6fRZtA1ogHiu22D+b8FX9AXyQ/eQ/IhOHu0oH3Mt 6, 41 -- 4QCaHHlPNv1/6ZDoAS5n6G+dyBQd0YQNTxArN72MjqY2vRyJCsqdAKqfWeWX6dSHUtKp 6, 41 -- +vOC+Uopx8iSs73L/OJf7M3Tda9srxycYjHr6w5mAno2JjN6CxCvgUUU3SmjHXtVpq5E 6, 41 -- +LRg== 6, 41 -- X-Gm-Message-State: AOPr4FWTFo8zfwP9Pf+8cOGs+ZZobNKpeKOc+pGqU+pxwc1poIQgjKBeWH6blCe1OA+o/H9ZV0pVSkTdXj8bDQ== 6, 41 -- X-Received: by 10.50.10.165 with SMTP id j5mr9842212igb.29.1462359706837; Wed, 6, 41 -- 04 May 2016 04:01:46 -0700 (PDT) 6, 41 -- MIME-Version: 1.0 6, 41 -- Received: by 10.107.26.68 with HTTP; Wed, 4 May 2016 04:01:27 -0700 (PDT) 6, 41 -- From: =?UTF-8?B?T25kxZllaiBLb3RlY2vDvQ==?= {ondrej.kotecky-at-gmail.com} 6, 41 -- Date: Wed, 4 May 2016 13:01:27 +0200 6, 41 -- Message-ID: {CAJRapMBCgjeRwZ2Vm0WRMcGrEaL83kiF+wh4O-yg_rFhHnL2CQ-at-mail.gmail.com} 6, 41 -- Subject: Cryomicrotomy using glass knives 6, 41 -- To: microscopy-at-microscopy.com 6, 41 -- Content-Type: text/plain; charset=UTF-8 6, 41 -- Content-Transfer-Encoding: 8bit 6, 41 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u44Ax0kR020028 ==============================End of - Headers==============================
The Laboratory Technician will be responsible for assisting and/or training scientific staff and students with Scanning Electron Microscopy, X-ray microanalysis, Laser Scanning Confocal Microscopy, Cathodoluminescence Spectroscopy, X-ray Computed Tomography, specimen preparation and image processing and printing; will maintain functionality and capability of lab equipment; will undergo periodic training on new technologies; will order supplies and track usage and expenditures; will interact with curatorial staff and students on diverse projects using microscopy and digital imaging in the fields of zoology, paleontology, anthropology, archeology, and earth and planetary sciences.
SEM (EDS+EBSD+CL) Micro-computed tomography Laser scanning confocal microscopy + Raman spectroscopy TEM
Don: I made a typo in my previous message. The correct model number for the KNF Mini Diaphragm Pump is N 84.3 ANI.
If you decide to use this pump you will need a base plate to mount it on and a housing to shield the cooling fan. I can supply drawings for versions of these items that I'v used previously that are easy and cheap to make.
You will probably also want some vacuum valves, and for this purpose I can recommend the mini ball valves (such as Model 4114T11, T13. T64) sold by McMaster Carr (mcmastercarr.com, 562-692-5911). They also sell a full line of tubing fittings for polyethylene tubing.
Wil Bigelow
==============================Original Headers============================== 4, 42 -- From bigelow-at-umich.edu Tue May 3 09:37:20 2016 4, 42 -- Received: from mail-wm0-f43.google.com (mail-wm0-f43.google.com [74.125.82.43]) 4, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u43EbKuj016160 4, 42 -- for {microscopy-at-microscopy.com} ; Tue, 3 May 2016 09:37:20 -0500 4, 42 -- Received: by mail-wm0-f43.google.com with SMTP id a17so43346876wme.0 4, 42 -- for {microscopy-at-microscopy.com} ; Tue, 03 May 2016 07:40:04 -0700 (PDT) 4, 42 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 42 -- d=umich.edu; s=google; 4, 42 -- h=message-id:date:from:user-agent:mime-version:to:subject 4, 42 -- :content-transfer-encoding; 4, 42 -- bh=s3P39u7sUzezq41+0Cdd4RSU3y7FLKHAYnE1Y2eOeD8=; 4, 42 -- b=YniaPX0DqdyExLahLTBKz+wCF3BtbtqKbFU/bTB0thhQHLnuT2f18hGNr3e6II2jwx 4, 42 -- uBOyPJlyzCNrmoZAr4p6xdyhoUt+kWaP1bZJigYzwdMT1hCxnyuuKj330tcLRalcGp2p 4, 42 -- s/qfgLBfiY0KqUfIkxVAECfZPJibkEEaR5VHM= 4, 42 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 42 -- d=1e100.net; s=20130820; 4, 42 -- h=x-gm-message-state:message-id:date:from:user-agent:mime-version:to 4, 42 -- :subject:content-transfer-encoding; 4, 42 -- bh=s3P39u7sUzezq41+0Cdd4RSU3y7FLKHAYnE1Y2eOeD8=; 4, 42 -- b=HSjAPtW0PLQI043x5FQ2xj3LidBNifOMteuyJww2ONQd/y9wJZwQBEaZLcTPZW4SXn 4, 42 -- PncnTEvVxGn+vcwasdemqNbhWNsY0pnlsyT/rp9COqNoC5elLBdyD2ewEelhe1NkbLnc 4, 42 -- JTMUamJMT+0Lbchtypn9ayG29zVY+q8z1qivgHawZZKcRaK6DjpEtjIcE31xSFTP/dhQ 4, 42 -- Xa8aofLh0ZNXGbgmW+xIy5+ydc7oV8bI6KYJA67qOjKmL+GmSs7LRhDanfy/HVEDAkCl 4, 42 -- MEOLxg5WHHkCUj83qZwSvQoAqcb/8pyX5De+or7SaNM3p/gX9utlRIFnMu7G3klaOe1C 4, 42 -- IOHA== 4, 42 -- X-Gm-Message-State: AOPr4FW3MkNjS+nHdbosHY0XMtuLJMgpD8FAPj5P+1ycG8qrmDVRni9dSsAG6kmT5+wuQib9 4, 42 -- X-Received: by 10.28.181.148 with SMTP id e142mr3694442wmf.38.1462286403026; 4, 42 -- Tue, 03 May 2016 07:40:03 -0700 (PDT) 4, 42 -- Received: from Wilbur-Bigelows-G4.local ([2602:306:3b93:aba0:230:65ff:fe6b:155c]) 4, 42 -- by smtp.gmail.com with ESMTPSA id lh1sm4279952wjb.20.2016.05.03.07.40.01 4, 42 -- for {microscopy-at-microscopy.com} 4, 42 -- (version=TLS1 cipher=ECDHE-RSA-AES128-SHA bits=128/128); 4, 42 -- Tue, 03 May 2016 07:40:02 -0700 (PDT) 4, 42 -- Message-ID: {5728B83F.5090107-at-umich.edu} 4, 42 -- Date: Tue, 03 May 2016 10:39:59 -0400 4, 42 -- From: Wil Bigelow {bigelow-at-umich.edu} 4, 42 -- User-Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X 10.4; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 4, 42 -- MIME-Version: 1.0 4, 42 -- To: microscopy-at-microscopy.com 4, 42 -- Subject: [Microscopy}Correction, Low Vac pump 4, 42 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 42 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I think perhaps the Model N 64.3 ANI Mini Diaphragm Pump manufactured by KNF Neuberger (www.knf.com/usa.htm) would fill the bill. It will produce a vacuum below 10 Torr, has a speed of about 4 l//min and costs less than $600. I've used one of these on a couple of systems I've designed in the past with very satisfactory results. Wil Bigelow
==============================Original Headers============================== 1, 42 -- From bigelow-at-umich.edu Mon May 2 21:22:15 2016 1, 42 -- Received: from mail-wm0-f51.google.com (mail-wm0-f51.google.com [74.125.82.51]) 1, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u432MECA015382 1, 42 -- for {microscopy-at-microscopy.com} ; Mon, 2 May 2016 21:22:15 -0500 1, 42 -- Received: by mail-wm0-f51.google.com with SMTP id g17so25750067wme.0 1, 42 -- for {microscopy-at-microscopy.com} ; Mon, 02 May 2016 19:24:56 -0700 (PDT) 1, 42 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 1, 42 -- d=umich.edu; s=google; 1, 42 -- h=message-id:date:from:user-agent:mime-version:to:subject 1, 42 -- :content-transfer-encoding; 1, 42 -- bh=JVKGanHbZYxc/0P8cM9WHSxyzk+oEpq4CQXApTw9uPs=; 1, 42 -- b=VFnx0Ac/WjRFyShe2yqugmgSZ1rF2qTWz/rJYbsqL4mei5cdH3Z+JCgq8efelChnh+ 1, 42 -- bufGI9XGA5XDn2TDkSk5XqdkdR3wQY5MPLePMUIppS3dnspu3VFRvMa4f0lGvN/wFf6m 1, 42 -- Hw+qjMHQR7rj8I9WCouembRA2tYIx5mjsRv+4= 1, 42 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 1, 42 -- d=1e100.net; s=20130820; 1, 42 -- h=x-gm-message-state:message-id:date:from:user-agent:mime-version:to 1, 42 -- :subject:content-transfer-encoding; 1, 42 -- bh=JVKGanHbZYxc/0P8cM9WHSxyzk+oEpq4CQXApTw9uPs=; 1, 42 -- b=bGFapPthLcKnRh+E3NVxmGaitSj6eNqcDJDo7tiqae9tgzBFJL0/u971gXWILU4+bp 1, 42 -- a0YEkEeqbJw/YiYbIJTJIkNSrRowQeUyKpGNtb79b2kaTLLqW7xtv7LI2EUZzXfnx0f6 1, 42 -- nNn/BiUpRB1rE+1Dl8zYwLdf2IaUoueBfKDuKnlkmEDL+iBQ8pwy2WhAQFiLqNcYd6wV 1, 42 -- +GARn3vUfYsiGGRnkxclQ0JSP7gTWkSAHpVLDSHxQtn3TZCwMcljL/sUx5GwMb41M+Qd 1, 42 -- aWAn9V/XN3a5+tbnDWqGlxNe9XtRkLiy60boDWKQfxLUuUvqE7SMiHsDVejsbOfQ4L6k 1, 42 -- dnQg== 1, 42 -- X-Gm-Message-State: AOPr4FVWpv+K5nkEUb3U+BDQohdidkgMMse7YZI98fylyIszZoQT5QepjQu/Euh3m91JXAju 1, 42 -- X-Received: by 10.194.77.140 with SMTP id s12mr3705105wjw.24.1462242295769; 1, 42 -- Mon, 02 May 2016 19:24:55 -0700 (PDT) 1, 42 -- Received: from Wilbur-Bigelows-G4.local ([2602:306:3b93:aba0:230:65ff:fe6b:155c]) 1, 42 -- by smtp.gmail.com with ESMTPSA id d1sm1059056wjb.47.2016.05.02.19.24.54 1, 42 -- for {microscopy-at-microscopy.com} 1, 42 -- (version=TLS1 cipher=ECDHE-RSA-AES128-SHA bits=128/128); 1, 42 -- Mon, 02 May 2016 19:24:55 -0700 (PDT) 1, 42 -- Message-ID: {57280BF4.3010008-at-umich.edu} 1, 42 -- Date: Mon, 02 May 2016 22:24:52 -0400 1, 42 -- From: Wil Bigelow {bigelow-at-umich.edu} 1, 42 -- User-Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X 10.4; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 1, 42 -- MIME-Version: 1.0 1, 42 -- To: microscopy-at-microscopy.com 1, 42 -- Subject: [Microscopy]Low Vacuum Pump 1, 42 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 1, 42 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I haven't had to buy a photo printer for micrographs for some years. What's the current best? HP doesn't make the Photosmart series anymore, and the Epson Sure color series specs look good, but ... ? I need both color and greyscale. Maybe the Epson P600? Thanks.
Hi Phil, and colleagues on the list, we have bought an Officejet Pro 8100 two weeks ago, and we have an Officejet Pro 8000 in use since a few years, directly at a TEM with 2k x 2k camera - to the delight of the users. no interest in the manufacturing company, of course ... kind regards, Reinhard
-- Prof. Dr. Reinhard Rachel University of Regensburg Centre for EM / Anatomy Faculty of Biology & Preclin. Med. Universitaetsstrasse 31 D-93053 Regensburg - Germany tel +49 941 943 -2837, -1720 mail reinhard.rachel-at-biologie.uni-regensburg.de office: VKL 3.1.29
Next microscopy conferences: - 16th Europ Microsc Congress EMC http://www.emc2016.fr/en/ 28 Aug - 2 Sept 2016 in Lyon, FR - Microscopy Conference 2017 Dreilaendertagung Lausanne, CH 20-25 August 2017 - next Microbiol. conferences: VAAM/DGHM - Annual Conf March 5-8, 2017, Wuerzburg
==============================Original Headers============================== 5, 25 -- From Reinhard.Rachel-at-biologie.uni-regensburg.de Mon May 2 02:31:45 2016 5, 25 -- Received: from rrzmta2.uni-regensburg.de (rrzmta2.uni-regensburg.de [194.94.155.52]) 5, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u427Vjvg031648 5, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 2 May 2016 02:31:45 -0500 5, 25 -- Received: from rrzmta2.uni-regensburg.de (localhost [127.0.0.1]) 5, 25 -- by localhost (Postfix) with SMTP id 2358A45F18 5, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 2 May 2016 09:34:19 +0200 (CEST) 5, 25 -- Received: from gwsmtp1.uni-regensburg.de (gwsmtp1.uni-regensburg.de [132.199.5.51]) 5, 25 -- by rrzmta2.uni-regensburg.de (Postfix) with ESMTP id 0B95F64F18 5, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 2 May 2016 09:34:18 +0200 (CEST) 5, 25 -- Received: from uni-regensburg-smtp1-MTA by gwsmtp1.uni-regensburg.de 5, 25 -- with Novell_GroupWise; Mon, 02 May 2016 09:34:21 +0200 5, 25 -- Message-Id: {57271F18020000540005E385-at-gwsmtp1.uni-regensburg.de} 5, 25 -- X-Mailer: Novell GroupWise Internet Agent 14.2.0 5, 25 -- Date: Mon, 02 May 2016 09:34:16 +0200 5, 25 -- From: "Reinhard Rachel" {Reinhard.Rachel-at-biologie.uni-regensburg.de} 5, 25 -- To: {oshel1pe-at-cmich.edu} , "microscopy listserver" {Microscopy-at-microscopy.com} 5, 25 -- Subject: Re: [Microscopy] Photo printers 5, 25 -- References: {201604291818.u3TIIUhN013081-at-ns.microscopy.com} 5, 25 -- In-Reply-To: {201604291818.u3TIIUhN013081-at-ns.microscopy.com} 5, 25 -- Mime-Version: 1.0 5, 25 -- Content-Type: text/plain; charset=US-ASCII 5, 25 -- Content-Disposition: inline 5, 25 -- Content-Transfer-Encoding: 8bit 5, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u427Vjvg031648 ==============================End of - Headers==============================
I'm working with a wafer inspection system that uses clean dry air to float a vibration isolation table and to release parts from vacuum clamps.
The manufacturer's specifications call for:
Pressure: 80-100 psi
Flow rate: { 1 cfm (28 l/m)
Cleanliness: Filtered to Class I
The actual flow will be intermittent.
I'm looking a quiet system that is easy to maintain. I would like to receive suggestions of specific equipment configurations.
regards, Don ============================================= Don Chernoff, Ph.D., President Advanced Surface Microscopy, Inc.; 3250 N. Post Rd., Suite 120; Indianapolis IN 46226 USA E-Mail: donc-at-asmicro.com www.asmicro.com Voice: +1-317-895-5630 Toll free: +1-800-374-8557 (in USA & Canada) Fax: +1-317-895-5652 [business activities since 1990: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.] =============================================
==============================Original Headers============================== 13, 36 -- From donc-at-asmicro.com Sun May 1 18:05:52 2016 13, 36 -- Received: from resqmta-ch2-05v.sys.comcast.net (resqmta-ch2-05v.sys.comcast.net [69.252.207.37]) 13, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u41N5q9g006642 13, 36 -- for {microscopy-at-microscopy.com} ; Sun, 1 May 2016 18:05:52 -0500 13, 36 -- Received: from resomta-ch2-18v.sys.comcast.net ([69.252.207.114]) 13, 36 -- by comcast with SMTP 13, 36 -- id x0TCaMHkGBE6Ox0TVauH7k; Sun, 01 May 2016 23:08:29 +0000 13, 36 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=comcast.net; 13, 36 -- s=q20140121; t=1462144109; 13, 36 -- bh=j0dmGOk5NI5qJ+b0IFtRaN3EYlxluzJp3a1o8e70ji0=; 13, 36 -- h=Received:Received:Message-ID:From:To:Subject:Date:MIME-Version: 13, 36 -- Content-Type; 13, 36 -- b=I8Ar48oxVMDq1oWmHrD21VlZygSVkr8vLAz8SR1AQb4uuI6/OwgzkAR4NzIY9dt9p 13, 36 -- 5Ozzr6L91kRnt0nzyesLNK5XeQ6A7u3lRV4xzJz0hhMsyFxLETe5MlEyKC7NPWnrcQ 13, 36 -- 6uDpkJQw1nx3q+S4SSgGWQBjG51y+F0I2dmbfhb4sFwC5BK9GB7kP3vNfygW8trENm 13, 36 -- x3Epyc0NSbH8IptlPlj0OY1lz0XbuT/tH5cueg7/1HlbQaQDpBF+1Qrm/0J680Q/rP 13, 36 -- bP9CX+yfforOHy5Eit4vqWwaOlaBc4zOABUHWnb1aUvdyfwqEf6Bk3kKW+q2+BR430 13, 36 -- hz3salvOa83Vg== 13, 36 -- Received: from asm20 ([68.45.155.184]) 13, 36 -- by resomta-ch2-18v.sys.comcast.net with comcast 13, 36 -- id pB8V1s0033yyn3Y01B8VdQ; Sun, 01 May 2016 23:08:29 +0000 13, 36 -- Message-ID: {4B176DE84D984487A7C58922082919BD-at-asm20} 13, 36 -- From: "Don Chernoff at ASM" {donc-at-asmicro.com} 13, 36 -- To: "Microscopy List" {microscopy-at-microscopy.com} 13, 36 -- Subject: Clean dry air 13, 36 -- Date: Sun, 1 May 2016 19:09:08 -0400 13, 36 -- MIME-Version: 1.0 13, 36 -- Content-Type: text/plain; 13, 36 -- format=flowed; 13, 36 -- charset="iso-8859-1"; 13, 36 -- reply-type=original 13, 36 -- Content-Transfer-Encoding: 7bit 13, 36 -- X-Priority: 3 13, 36 -- X-MSMail-Priority: Normal 13, 36 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5931 13, 36 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6157 ==============================End of - Headers==============================
I'm working with a wafer inspection system that uses vacuum to clamp the wafer. The manufacturer's specifications call for a modest vacuum:
low vacuum: 150 torr (-24" Hg)
flow rate: { 1 cfm (28 l/m)
Actual flow will be intermittent.
What type of pump would be suitable for this application?
I welcome suggestions for specific models and suggestions for how to configure the pump.
regards, Don ============================================= Don Chernoff, Ph.D., President Advanced Surface Microscopy, Inc.; 3250 N. Post Rd., Suite 120; Indianapolis IN 46226 USA E-Mail: donc-at-asmicro.com www.asmicro.com Voice: +1-317-895-5630 Toll free: +1-800-374-8557 (in USA & Canada) Fax: +1-317-895-5652 [business activities since 1990: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.] =============================================
==============================Original Headers============================== 10, 36 -- From donc-at-asmicro.com Sun May 1 17:58:18 2016 10, 36 -- Received: from resqmta-ch2-07v.sys.comcast.net (resqmta-ch2-07v.sys.comcast.net [69.252.207.39]) 10, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u41MwITE003272 10, 36 -- for {microscopy-at-microscopy.com} ; Sun, 1 May 2016 17:58:18 -0500 10, 36 -- Received: from resomta-ch2-16v.sys.comcast.net ([69.252.207.112]) 10, 36 -- by comcast with SMTP 10, 36 -- id x0M1axB8sp1Bex0MBa2Ax0; Sun, 01 May 2016 23:00:55 +0000 10, 36 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=comcast.net; 10, 36 -- s=q20140121; t=1462143655; 10, 36 -- bh=5YKlv29KfB2X47BOX6fSUaVPqjafQm4cRuCFouKT2o4=; 10, 36 -- h=Received:Received:Message-ID:From:To:Subject:Date:MIME-Version: 10, 36 -- Content-Type; 10, 36 -- b=F4GJAlSZN4QYJMr7WmlCx4WGIS4d5rRo1dsO7Rue/eg6FwvjZ5ciVYBc6uP7IKK2/ 10, 36 -- h/M9ZkU/lqd2QcEJmkfeZhVynFNMY6XHSPTFt3Hg1y8+yc/HVnxtpl+XtqA65SNCxe 10, 36 -- E67BUiY1IvBpRbkwKiiZkaJ9kn6wwf+qLyhD1HuJLAwHS9GCzgbgw9lJMzGteOw0/8 10, 36 -- 43kSF1CKYZep+yn5vN5iCquMZJhPlEOOwFptPRHVhrcbAL7SSoLjDHPFG1MkfBkGcJ 10, 36 -- B4tE+4bxaqdp//lBg331TGmlngEZM31der8NUO0pd9RXJ6tzLLY0oKG8bzMOo4UdbO 10, 36 -- 8glqJjYMhmV0g== 10, 36 -- Received: from asm20 ([68.45.155.184]) 10, 36 -- by resomta-ch2-16v.sys.comcast.net with comcast 10, 36 -- id pB0u1s00C3yyn3Y01B0u4y; Sun, 01 May 2016 23:00:55 +0000 10, 36 -- Message-ID: {EE952F34508646FA93AD17ECFA9405A1-at-asm20} 10, 36 -- From: "Don Chernoff at ASM" {donc-at-asmicro.com} 10, 36 -- To: "Microscopy List" {microscopy-at-microscopy.com} 10, 36 -- Subject: low vacuum pump 10, 36 -- Date: Sun, 1 May 2016 19:01:31 -0400 10, 36 -- MIME-Version: 1.0 10, 36 -- Content-Type: text/plain; 10, 36 -- format=flowed; 10, 36 -- charset="iso-8859-1"; 10, 36 -- reply-type=original 10, 36 -- Content-Transfer-Encoding: 7bit 10, 36 -- X-Priority: 3 10, 36 -- X-MSMail-Priority: Normal 10, 36 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5931 10, 36 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6157 ==============================End of - Headers==============================
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Email: rebecca.jackson-at-utsouthwestern.edu Name: Rebecca Jackson
Organization: UT Southwestern
Title-Subject: [Filtered] Fume hood extension arm
Message: Hey all- We are looking into getting a fume hood extension/extraction arm in our EM lab, hopefully making it easier and safer for cutting tissues in fix using the dissecting scope. We hope to do this on the bench with the arm right next to the dish containing the tissue and fix while using the dissecting scope. Does anyone have experience with these? Could I get some info on what you have or if these even work well for harsh chemicals? Or do you do/use something totally different....? Thanks all!!
-Bec.
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We have a student, that would like to work with a polymer that if 100% polymerized would be ok, but seldom are polymer's 100% polymerized. In other physical states this polymer is toxic.
They hope to make this into a thin film, and use it in x-ray, SEM and TEM.
The toxin/ sensitizer is ethylviologen (ethyl iodide), a bio based substrate known to be a sensitizer, the polymer has the base of Polyvinylbenzylchloride, 4-4-bipyridine, and could include ethylviologen monomers and dipyridyl monomers.
SUGGESTED PRECAUTIONS ALREADY: Vacuum for a week, mild heat to help gas off, buying own sample stubs and bringing already prepped. Test just plain polybenzylchloride to watch for sublimation.
Desired instruments of use: SEM, TEM, X-ray
QUESTIONS:
* Even if vacuum is pre-applied for as long as a week in their own lab, will such a product gas out into the TEM causing all the insides to be contaminated?
* Even if vacuum is not a problem, what about sublimation of the polymer by the beam?
* What are your standard precautions for soft polymers, especially those that are toxic? What are the limits of materials you allow in your EM scopes?
Thanks for any help,
Lou Ann
{ { { { { { { { { { { { { { { { {} } } } } } } } } } } } } } } } Lou Ann Miller Biological Electron Microscopy Frederick Seitz Materials Research Laboratory Lab Room 125 Office 374 MRL 104 South Goodwin Ave Urbana, IL 61801 Campus mail code: MC-230 Hours: 7am-3:30 pm
I haven't had to buy a photo printer for micrographs for some years. What's the current best? HP doesn't make the Photosmart series anymore, and the Epson Sure color series specs look good, but ... ? I need both color and greyscale. Maybe the Epson P600? Thanks.
Phil -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 3, 33 -- From oshel1pe-at-cmich.edu Fri Apr 29 13:17:59 2016 3, 33 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 3, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u3TIHx48012606 3, 33 -- for {microscopy-at-microscopy.com} ; Fri, 29 Apr 2016 13:17:59 -0500 3, 33 -- Received: from cas.cmich.edu (async2.cmich.edu [141.209.15.141] (may be forged)) 3, 33 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id u3TIKRCS001795 3, 33 -- for {microscopy-at-microscopy.com} ; Fri, 29 Apr 2016 14:20:27 -0400 3, 33 -- Received: from cas4.central.cmich.local (2002:8dd1:f03::8dd1:f03) by 3, 33 -- cas2.central.cmich.local (2002:8dd1:f8d::8dd1:f8d) with Microsoft SMTP Server 3, 33 -- (TLS) id 14.3.248.2; Fri, 29 Apr 2016 14:20:28 -0400 3, 33 -- Received: from CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) by 3, 33 -- CAS4.central.cmich.local (2002:8dd1:f03::8dd1:f03) with Microsoft SMTP Server 3, 33 -- (TLS) id 14.3.248.2; Fri, 29 Apr 2016 14:20:27 -0400 3, 33 -- Received: from bio-br024c-mac01.local (141.209.2.100) by mail.cmich.edu 3, 33 -- (141.209.15.90) with Microsoft SMTP Server (TLS) id 14.3.248.2; Fri, 29 Apr 3, 33 -- 2016 14:20:27 -0400 3, 33 -- Message-ID: {5723A5EB.8070708-at-cmich.edu} 3, 33 -- Date: Fri, 29 Apr 2016 14:20:27 -0400 3, 33 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 3, 33 -- Reply-To: {oshel1pe-at-cmich.edu} 3, 33 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 3, 33 -- MIME-Version: 1.0 3, 33 -- To: micro {microscopy-at-microscopy.com} 3, 33 -- Subject: Photo printers 3, 33 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 3, 33 -- Content-Transfer-Encoding: 7bit 3, 33 -- X-Originating-IP: [141.209.2.100] 3, 33 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 3, 33 -- X-Spam-Score: -1.70 () [Hold at 6.00] RDNS_NONE,_L_LEXNRL,_L_LLEXCH,SPF(softfail:0),RBL(rp-good:-0.1),Bayes(0.0001:-0.5) 3, 33 -- X-CanIt-Geo: ip=141.209.15.141; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6 3, 33 -- X-CanItPRO-Stream: default 3, 33 -- X-Canit-Stats-ID: 02QMikr4G - e511e4dc73f0 - 20160429 3, 33 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
Note! Please do *not* use "Reply"! Send any response to the list or to John L. Grazul {jlg98-at-cornell.edu} . I'm sending this for a colleague locked behind a Cornell Curtain. Phil
LaB6 enthusiasts! This afternoon I am looking at 4 Kimball filaments in the FIB. Some had a dignified normal death, others an untimely and less dignified death. What we are going to look at is the elemental differences between them, from the La right to the base. We are going to do slice and views and look for defects. Is there anything else you guys want us to investigate? Let me know and your dreams will come true, we are the Disneyland of microscopy.
I have been contacted by one of the manufacturers, they too are interested in the data we get. So things are moving and hopefully we will be getting good product in the near future.
Next on my agenda will be the quality and performance of FEG tips. How have your FEG tips been lately?? We at Cornell have tales to tell. Send me your tales of woe or post them, but let s just say our experience lately has been similar to our issues with LaB6.
Thanks for interacting, and if we don t keep the suppliers on their toes, our down time because of poor product will just be expected rather than a surprise.
jg
==============================Original Headers============================== 7, 30 -- From oshel1pe-at-cmich.edu Fri Apr 29 11:24:14 2016 7, 30 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 7, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u3TGOELT012018 7, 30 -- for {microscopy-at-microscopy.com} ; Fri, 29 Apr 2016 11:24:14 -0500 7, 30 -- Received: from cas.cmich.edu (mail.cmich.edu [141.209.15.40] (may be forged)) 7, 30 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id u3TGQgkq020469 7, 30 -- for {microscopy-at-microscopy.com} ; Fri, 29 Apr 2016 12:26:42 -0400 7, 30 -- Received: from CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) by 7, 30 -- cas1.central.cmich.local (2002:8dd1:f28::8dd1:f28) with Microsoft SMTP Server 7, 30 -- (TLS) id 14.3.248.2; Fri, 29 Apr 2016 12:26:43 -0400 7, 30 -- Received: from bio-br024c-mac01.local (141.209.2.100) by mail.cmich.edu 7, 30 -- (141.209.15.90) with Microsoft SMTP Server (TLS) id 14.3.248.2; Fri, 29 Apr 7, 30 -- 2016 12:26:42 -0400 7, 30 -- Message-ID: {57238B42.7080700-at-cmich.edu} 7, 30 -- Date: Fri, 29 Apr 2016 12:26:42 -0400 7, 30 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 7, 30 -- Reply-To: {oshel1pe-at-cmich.edu} 7, 30 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 7, 30 -- MIME-Version: 1.0 7, 30 -- To: micro {microscopy-at-microscopy.com} 7, 30 -- Subject: Fwd: LaB6 Update: Could use some suggestions 7, 30 -- Content-Type: text/plain; charset="windows-1252"; format=flowed 7, 30 -- Content-Transfer-Encoding: 8bit 7, 30 -- X-Originating-IP: [141.209.2.100] 7, 30 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 7, 30 -- X-Spam-Score: -0.30 () [Hold at 6.00] RDNS_NONE,_L_LLEXCH,SPF(softfail:0),Bayes(0.0001:-0.5) 7, 30 -- X-CanIt-Geo: ip=141.209.15.40; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6 7, 30 -- X-CanItPRO-Stream: default 7, 30 -- X-Canit-Stats-ID: 02QMgqGeS - 8b3b80445ea6 - 20160429 7, 30 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
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Email: jzheng-at-uci.edu Name: Jian-Guo Zheng
Organization: IMRI UCI
Title-Subject: [Filtered] Project Scientist Series Electron Microscopy Scientific Staff -positions open at UC Irvine
Message: For details, please visit https://recruit.ap.uci.edu/apply/JPF03381
RECRUITMENT PERIOD Open April 20th, 2016 through June 30th, 2016 DESCRIPTION
Project Scientist Series -Electron Microscopy Scientific Staff
Salary-Commensurate with experience
POSTING DATE: APRIL 20, 2016 CLOSING DATE: OPEN UNTIL FILLED
DESCRIPTION Manage the day-to-day operations and help guide the strategic build-out of the new transmission electron microscopy (TEM) facility at the UC Irvine Materials Research Institute (IRMI). IMRI has a large and growing user community of over 300 academic and industry users, with advanced materials characterization instruments that include state-of-the-art TEMs, scanning electron microscopes (SEMs), focused ion beam (FIB) systems, and a Kratos AXIS Supra surface science instrument (for details see http://lexi.eng.uci.edu). IMRI has recently built a new TEM facility housing five major instruments, including four high-end TEMs (Nion UltraSTEM 200 HERMES, JEOL JEM-ARM300CF Grand ARM, JEM-2800, and JEM-2100F Cryo-TEM) and a dual-beam FIB (Tescan GAIA-3 XMH FIB-SEM). IMRI will be the first lab in the Americas with the newly introduced JEOL Grand ARM and the Nion s high-performance UltraSTEM 200 HERMES, which provides an energy resolution for electron energy loss spectroscopy (EELS) of 7 meV or better. Both the Grand ARM and the 2100F Cryo-TEM will be equipped with Gatan s K2 cameras. IMRI is also equipped with a variety of TEM in situ holders and specimen preparation tools. Two TEM scientific staff positions are available: (1) TEM specialist for materials science applications and (2) Cryo-TEM specialist for biological and soft materials. The successful applicant must hold PhD or equivalent degrees in the areas of Materials Science, Physics, Chemistry or biology, and be TEM experts with at least five years of in-depth, hands-on experience with TEM/STEM imaging, spectroscopy, TEM sample preparation, and maintenance of TEM equipment. Applicants who are merely users of TEM will not be considered. The applicant must have mastery of modern TEM/STEM instrumentation and methods, data collection, analysis, and interpretation. Candidates with experience operating user facilities and expertise with advanced TEM/STEM imaging, spectroscopy, and in-situ microscopy techniques are of particular interest. Duties include: operate, maintain, and improve the TEMs; work with the IMRI leadership to identify and obtain accessories and additional equipment for a phased build-out of the TEM facility; train, supervise, and work directly with users to carry out research; co-manage all aspects of day-to-day IMRI operations; participate in teaching and outreach activities; promote IMRI on and off campus; secure grants, perform original research, attend scientific conferences, and publish scientific research in peer-reviewed journals. The University of California, Irvine is an Equal Opportunity/Affirmative Action Employer advancing inclusive excellence. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age, protected veteran status, or other protected categories covered by the UC nondiscrimination policy. TO LEARN MORE AND APPLY Apply by submitting your application to our online RECRUIT system at: https://recruit.ap.uci.edu/apply/JPF03381 More information about this recruitment: www.calit2.uci.edu JOB LOCATION Irvine, CA REQUIREMENTS DOCUMENTS Curriculum Vitae - Your most recently updated C.V. Cover Letter Statement of Research Statement of Teaching (Optional) Statement of Contributions to Diversity - Statement addressing how past and/or potential contributions to diversity will advance UCI's Commitment to Inclusive Excellence. Misc / Additional (Optional) REFERENCES 3-5 letters of reference required HOW TO APPLY 1. Create an ApplicantID 2. Provide required information and documents 3. If any, provide required reference information
To apply these positions, please visit https://recruit.ap.uci.edu/apply/JPF03381 Thanks!
Jian-Guo Zheng, PhD, FRMS IMRI Director of Facilities Irvine Materials Research Institute University Of California, Irvine 3421 Calit2 Building Irvine, CA 92697-2800 USA Telephone: 949-824-0441 (office), 949-468-9980 (cell) Fax: 949-824-8195 Email: jzheng-at-uci.edu
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I've already sent you a manual for the EMS 850 CPD but can give you some pointers that are not included in the manual.
What CO2 tank size do you use? Typically your gas supplier will deliver a J-size tank but it MUST have a "Dip tube or Siphon tube" for any CPD to function. The siphon tube draws liquid Co2 from the bottom of the tank, without this you would only draw gas.
What support items do you use? Plastic waste containers to contain Alcohol waste. Small plastic flowmeter to measure the flow of Co2 out of the pressure vessel during the final stage of drying. There are several types of sample holders depending on application, Everything from bulk samples to coverslip holders are in the EMS 850 section of our catalog.
This unit comes with a SST reinforced, 1500psi certified transfer hose. If you didn't get one with the used 850, contact me directly for a replacement.
How automatic is the system? The EMS 850 is semi-automatic. See the details of operation in the manual.
For putting bio specimens into the SEM, how do you factor-in this CPD before going into the chamber? Not sure I understand what the question is. All biological samples must be dried* before coating or insertion into the SEM
Al Coritz (acoritz-at-emsdiasum.com) Applications & Service Manager EMS Technical Service
www.emsdiasum.com 800-523-5874 1560 Industry Road Hatfield, PA 19440
Note! Please do *not* use "Reply"! Send any response to the list or to John L. Grazul {jlg98-at-cornell.edu} . I'm sending this for a colleague locked behind a Cornell Curtain. Phil
Message: Fellow microscopists I have a follow up on our LaB6 issue and a suggestion that should work from one of our microscopy luminaries.
There is correspondence going out to Denka and Kimball regarding our issues, with our concerns and observations attached, from one of the major suppliers of microscopy consumables. Hopefully there will be some movement or at least an acknowledgement that Oh yeah, we did change our La supplier and welding technique and who we are outsourcing manufacturing to . Don t think you are the only one having issues guys! Throw something up on the list and at the least you will get that reality check that you are not losing your mojo, or the reality check that indeed you ARE losing your mojo.
Regarding the manual alignment phenomena; if you recall, we align the Lab6 on the bench as usual, put the gun in the scope, heat it up and the deflectors can t center the tip, we max out. Take the gun out and the manual alignment looks great. Henk Colijn theorizes that the resistance is not the same on the posts so as they heat up the tip gets pulled to one side or the other. Earl Kirkland suggested that we anneal the filament before we manually align, which is what we do for our cold FEGs. I gave it a go with my latest Lab6: ran the HT to 120kV and ran a dribble current through the crystal over the weekend, getting it good and hot, manually aligned and so far so good. BUT we should not have to do this because in the recent past we never had to do this. In our case it is more downtime which ultimately adds to the cost of the filament.
So guys, I will keep you posted on what happens, any other observations or suggestions please pass on to our community. Some issues may seem trivial but we all have a common bond, and our collective mojo is a pretty awesome force.
jg
==============================Original Headers============================== 7, 30 -- From oshel1pe-at-cmich.edu Thu Apr 28 08:45:50 2016 7, 30 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116]) 7, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u3SDjoFx009525 7, 30 -- for {microscopy-at-microscopy.com} ; Thu, 28 Apr 2016 08:45:50 -0500 7, 30 -- Received: from cas.cmich.edu (async2.cmich.edu [141.209.15.141] (may be forged)) 7, 30 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id u3SDmDod000479 7, 30 -- for {microscopy-at-microscopy.com} ; Thu, 28 Apr 2016 09:48:14 -0400 7, 30 -- Received: from cas1.central.cmich.local (2002:8dd1:f28::8dd1:f28) by 7, 30 -- cas2.central.cmich.local (2002:8dd1:f8d::8dd1:f8d) with Microsoft SMTP Server 7, 30 -- (TLS) id 14.3.248.2; Thu, 28 Apr 2016 09:48:15 -0400 7, 30 -- Received: from bio-br024c-mac01.local (141.209.2.100) by mail.cmich.edu 7, 30 -- (141.209.15.40) with Microsoft SMTP Server (TLS) id 14.3.248.2; Thu, 28 Apr 7, 30 -- 2016 09:48:14 -0400 7, 30 -- Message-ID: {5722149E.7020302-at-cmich.edu} 7, 30 -- Date: Thu, 28 Apr 2016 09:48:14 -0400 7, 30 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 7, 30 -- Reply-To: {oshel1pe-at-cmich.edu} 7, 30 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 7, 30 -- MIME-Version: 1.0 7, 30 -- To: micro {microscopy-at-microscopy.com} 7, 30 -- Subject: Fwd: LaB6 update and some suggestions 7, 30 -- Content-Type: text/plain; charset="windows-1252"; format=flowed 7, 30 -- Content-Transfer-Encoding: 8bit 7, 30 -- X-Originating-IP: [141.209.2.100] 7, 30 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 7, 30 -- X-Spam-Score: -1.70 () [Hold at 6.00] L_CTCMUM,L_TMAILSRV,RDNS_NONE,_L_LEXNRL,_L_LLEXCH,SPF(softfail:0),RBL(rp-good:-0.1),Bayes(0.0001:-0.5) 7, 30 -- X-CanIt-Geo: ip=141.209.15.141; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6 7, 30 -- X-CanItPRO-Stream: default 7, 30 -- X-Canit-Stats-ID: 05QLNMdBw - f6f14fd4f1be - 20160428 7, 30 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.15.116 ==============================End of - Headers==============================
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Title-Subject: [Filtered] Current EM Techniques Workshop on May 24 & 25
Message: Dear Colleagues,
We would like to remind you that the registration of the UMB Current EM Techniques Workshop (05/24-05/25) is OPEN. The purpose of this annual workshop is to introduce participants to new electron microscopy techniques and instruments via live demonstration and open discussion. The focus of this year s workshop is Correlative LM & EM. The workshop will include oral presentations, Tips-and-Tricks Technology forums on the first day and live instrument demonstration on the second day. Dr. Lucy Collison of Francis Crick Institute and Richard Schalek of Harvard University will be the keynote speakers. Demo instruments will feature ATUMtome of RMC-Boeckeler, ASP-1000-IGL of Microscopy Innovations, Pelco Biowave Pro Microwave System of TedPella, Cryo EM sample preparation of Leica Microsystems, Correlative Array Tomography of Zeiss, Polyo III, Lynx Tissue Processor of EMS, Aerosurf Atmospheric SEM of Hitachi/Angstrom.
A joint dinner event with the Chesapeake Microscopy and Microanalysis society (CMMS) on May 24th provides opportunities for social and scientific exchange among workshop participants and CMMS members.
More information: http://www.dental.umaryland.edu/2016currentemtechniquesworkshop/
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I am preparing mouse livers for TEM the classical way: fixation glutar+Formaldehyde, Osmium post-fixation, embedding in Epon. Some liver samples are loaded with fat, mainly due to a treatment the mice received. The sample were left several weeks in fixative at 4*C before processing so extraction of at least some fat would not be a surprise.
Now on semithin sections stained with methylene blue/Azur II the fat globules have a greenish blue appearance. I thought it was due to the presence of osmium. However, when I observe ultrathin sections of the same samples in TEM, the fat globule are empty, I see nothing, it is only resin inside so they are probably extracted. My question is: how can the fat be colored in semi-thin sections and empty/extracted in ultrathin section? How do you explain de greenish blue color with methylene blue/azur II?
Regards, Stephane
==============================Original Headers============================== 5, 30 -- From nizets2-at-yahoo.com Thu Apr 28 02:56:20 2016 5, 30 -- Received: from nm32-vm7.bullet.mail.bf1.yahoo.com (nm32-vm7.bullet.mail.bf1.yahoo.com [72.30.239.143]) 5, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u3S7uJFJ019541 5, 30 -- for {microscopy-at-microscopy.com} ; Thu, 28 Apr 2016 02:56:20 -0500 5, 30 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1461830324; bh=JKYEh/Nb7z1jDUPskNBJleAOwbR37AR7SCKdl0LaPa0=; h=Date:From:Reply-To:To:Subject:References:From:Subject; b=c+Tr9DwF6k6g0MSWRJs0oTTwD9VminoLotDyXjl8x8VyOjB60zpdSbGv4HbnnCbuv/FjR1ntzKbpB6yIYL5hlWHSkMCBN0TIhOg48TtKTw5C2vTc6evUUNN1A3P3IVRikTjFhZU019eIMZZBHz+yCc5+tJGbAxwRj5B86NMDX9YoL0Av5blnrLaAfmgujCHIa4GHbBoFiLz/fEXsyBkDV4tCWf8E1UTNUJTdvPWsZ6ryrq2LyM8R7z7lDwYWSQlaNq1aDkMqD6rO6S59sNUH6YPbrPGwYaWKXPQtQUngCi/3dTvjXzEwyYJjXb3byTCmwy/6EOUTsfaGTgiVDuUD7Q== 5, 30 -- Received: from [98.139.214.32] by nm32.bullet.mail.bf1.yahoo.com with NNFMP; 28 Apr 2016 07:58:44 -0000 5, 30 -- Received: from [98.139.212.211] by tm15.bullet.mail.bf1.yahoo.com with NNFMP; 28 Apr 2016 07:58:44 -0000 5, 30 -- Received: from [127.0.0.1] by omp1020.mail.bf1.yahoo.com with NNFMP; 28 Apr 2016 07:58:44 -0000 5, 30 -- X-Yahoo-Newman-Property: ymail-3 5, 30 -- X-Yahoo-Newman-Id: 33956.30784.bm-at-omp1020.mail.bf1.yahoo.com 5, 30 -- X-YMail-OSG: qXIR1BcVM1m2zt7pjvnoeFCGSPKW_3KeP3rfp5ylNjkSbyAxs3InvvHhp7u7ONR 5, 30 -- 8RsKBerL6w74Ih5C740SC273MEAEOfv4LP_e01qu5WwdnGR8ogSZ6oHFQAJgJkFbXTha_eH8Rs7m 5, 30 -- AxHtg4EI7Pk6kzu5kIg24Ou7op6Es213edNdZOIA54H.Ew8VVwdzkExhbh1t18lKfaxWeUkNdLZI 5, 30 -- Y9mZRezrl8SzpYX1DGeJ_zC3wVz0MdHSHbpaG5U5U5EkRh5lxmpuNjXwJLSUus_Se5IbAw7exYrK 5, 30 -- zFZfI8OYGJAMDXS1oDhA47zwCMwtQXiUv282VJ7tJeKoWTBaGDvMDtAIJjb.fqIt6uEHHK2z0T1R 5, 30 -- X.j2VKFBbaJvQS.OqoPmItEFJCPRk8zocQMtLXV7gVRC_iXeoZOdz6PcgS6Ywf6ARcWCT3zP1bVh 5, 30 -- 93e8RVO7m34gvhnW4LThGZ6vj92gJYvurGyh.Q32ErY9yiVTNVufv.3IdebZ4M.B5FQocnuirzZO 5, 30 -- PD83R 5, 30 -- Received: by 66.196.80.122; Thu, 28 Apr 2016 07:58:43 +0000 5, 30 -- Date: Thu, 28 Apr 2016 07:58:43 +0000 (UTC) 5, 30 -- From: Stephane Nizet {nizets2-at-yahoo.com} 5, 30 -- Reply-To: Stephane Nizet {nizets2-at-yahoo.com} 5, 30 -- To: {microscopy-at-microscopy.com} 5, 30 -- Message-ID: {1594685055.3925265.1461830323263.JavaMail.yahoo-at-mail.yahoo.com} 5, 30 -- Subject: preparation of fat in mouse liver specimen 5, 30 -- MIME-Version: 1.0 5, 30 -- Content-Type: text/plain; charset=UTF-8 5, 30 -- References: {1594685055.3925265.1461830323263.JavaMail.yahoo.ref-at-mail.yahoo.com} 5, 30 -- Content-Transfer-Encoding: 8bit 5, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u3S7uJFJ019541 ==============================End of - Headers==============================
I'm tring to get a used EMS850 CPD up and running and wonder what others' experiences have been with this unit. This unit is before the version with a single dial gauge and digital readout.
What CO2 tank size do you use? What support items do you use? What hose works for you? How automatic is the system?
For putting bio specimens into the SEM, how do you factor-in this CPD before going into the chamber?
All input is appreciated.
TIA, gary g.
==============================Original Headers============================== 6, 26 -- From gary-at-gaugler.com Wed Apr 27 20:31:08 2016 6, 26 -- Received: from wavecable.net (cgfe-34-tuk.wavecable.net [66.235.59.183]) 6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u3S1V8IJ017836 6, 26 -- for {microscopy-at-microscopy.com} ; Wed, 27 Apr 2016 20:31:08 -0500 6, 26 -- Received: from [172.16.141.11] (HELO wavecable.net) 6, 26 -- by cgfe-3-tuk.wavecable.net (CommuniGate Pro SMTP 6.1.8) 6, 26 -- with ESMTP id 4213441 for microscopy-at-microscopy.com; Wed, 27 Apr 2016 18:26:38 -0700 6, 26 -- X-Junk-Score: 0 [] 6, 26 -- X-Cloudmark-Score: 0 [] 6, 26 -- X-Cloudmark-Analysis: v=2.1 cv=UdusM/mN c=1 sm=1 tr=0 a=2VnlTcbn8LW/DKys2mm+zQ==:117 a=IkcTkHD0fZMA:10 a=SEOrLOue2igA:10 a=kziv93cY1bsA:10 a=3T1MYC1FAxPeSyEvPIUA:9 a=QEXdDO2ut3YA:10 6, 26 -- Received: from [192.92.0.19] (HELO [127.0.0.1]) 6, 26 -- by wavecable.com (CommuniGate Pro SMTP 6.1.8) 6, 26 -- with ESMTP id 76122850 for microscopy-at-microscopy.com; Wed, 27 Apr 2016 18:26:38 -0700 6, 26 -- From: Gary Gaugler {gary-at-gaugler.com} 6, 26 -- Subject: EMS850 CPD use 6, 26 -- Reply-To: gary-at-gaugler.com 6, 26 -- To: microscopy-at-microscopy.com 6, 26 -- Message-ID: {57216861.8000008-at-gaugler.com} 6, 26 -- Date: Wed, 27 Apr 2016 18:33:21 -0700 6, 26 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:38.0) Gecko/20100101 6, 26 -- Thunderbird/38.3.0 6, 26 -- MIME-Version: 1.0 6, 26 -- Content-Type: text/plain; charset=utf-8; format=flowed 6, 26 -- Content-Transfer-Encoding: 7bit 6, 26 -- X-Antivirus: avast! (VPS 160427-0, 04/26/2016), Outbound message 6, 26 -- X-Antivirus-Status: Clean ==============================End of - Headers==============================
Hi Everyone, We are planning a field canceling system for the room where we will house an FEI Talos. There is the possibility of building the coils into the microscope enclosure - has anyone done that? Looking to hear experiences.
Thanks
Chris
Christopher J. Gilpin Ph.D. Director, Life Science Microscopy Facility Purdue University Whistler Hall of Agriculture Research, Room S052 170 S. University St West Lafayette, IN 47907 765-494-7750 gilpin-at-purdue.edu lsmf-at-purdue.edu reaches everyone in the facility
==============================Original Headers============================== 7, 33 -- From gilpin-at-purdue.edu Wed Apr 27 16:03:43 2016 7, 33 -- Received: from mailhub129.itcs.purdue.edu (mailhub129.itcs.purdue.edu [128.210.5.129]) 7, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u3RL3hZg025450 7, 33 -- for {microscopy-at-microscopy.com} ; Wed, 27 Apr 2016 16:03:43 -0500 7, 33 -- Received: from exchange.purdue.edu (exchange.purdue.edu [128.210.1.29]) 7, 33 -- by mailhub129.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id u3RL65n9028670 7, 33 -- for {microscopy-at-microscopy.com} ; Wed, 27 Apr 2016 17:06:05 -0400 7, 33 -- Received: from wppexc03.purdue.lcl (172.30.136.176) by wppexc03.purdue.lcl 7, 33 -- (172.30.136.176) with Microsoft SMTP Server (TLS) id 15.0.1156.6; Wed, 27 Apr 7, 33 -- 2016 17:06:05 -0400 7, 33 -- Received: from wppexc03.purdue.lcl ([fe80::3c86:dcb8:fd99:f174]) by 7, 33 -- wppexc03.purdue.lcl ([fe80::3c86:dcb8:fd99:f174%15]) with mapi id 7, 33 -- 15.00.1156.000; Wed, 27 Apr 2016 17:06:05 -0400 7, 33 -- From: "Gilpin, Christopher J" {gilpin-at-purdue.edu} 7, 33 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 7, 33 -- Subject: Field canceling systems in a Talos enclosure 7, 33 -- Thread-Topic: Field canceling systems in a Talos enclosure 7, 33 -- Thread-Index: AdGgyF5K4LgsYozqReCcJVLTt1j3RQ== 7, 33 -- Date: Wed, 27 Apr 2016 21:06:04 +0000 7, 33 -- Message-ID: {0d66719e56744cf3bcefdb215588672d-at-wppexc03.purdue.lcl} 7, 33 -- Accept-Language: en-US 7, 33 -- Content-Language: en-US 7, 33 -- X-MS-Has-Attach: 7, 33 -- X-MS-TNEF-Correlator: 7, 33 -- x-ms-exchange-transport-fromentityheader: Hosted 7, 33 -- x-originating-ip: [10.163.23.208] 7, 33 -- Content-Type: text/plain; charset="utf-8" 7, 33 -- MIME-Version: 1.0 7, 33 -- X-PMX-Version: 6.0.2.2308539 7, 33 -- X-PerlMx-URL-Scanned: Yes 7, 33 -- X-PerlMx-Virus-Scanned: Yes 7, 33 -- Content-Transfer-Encoding: 8bit 7, 33 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id u3RL3hZg025450 ==============================End of - Headers==============================
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Email: james.weston-at-nyu.edu Name: James Weston
Organization: New York University Abu Dhabi
Title-Subject: [Filtered] Electron microscopy position open
Message: Hello, We have recently posted a position for a research instrumentation specialist in electron microscopy.
This is a permanent staff position. The individual would be responsible for three electron microscopes (SEM, TEM, Dual beam).
Please pass on the announcement to colleagues who are not subscribers to this list service
Thanks! James
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Email: jhyun-at-gatan.com Name: John Hyun
Organization: Gatan, Inc.
Title-Subject: [Filtered] EELS and EFTEM Workshop at Harvard University
Message: 10:00 am - 6:00 pm, May 24, 2016 Center for Nanoscale Systems (CNS), Room 303, Laboratory for Integrated Science and Engineering (LISE) Building, Harvard University, MA
This workshop combines presentations and extensive hands-on training on advanced EELS and EFTEM techniques and applications in the (S)TEM microscope. Practical experience with EELS is highly recommended. Participants will learn best practices to set up and optimize their EELS hardware and experimental protocols so they can capture and extract the maximum amount of compositional and chemical information from their TEM samples.
Topics include:
-Advanced EELS acquisition techniques extended dynamic range and DualEELS -Advanced EELS processing techniques, MLLS and NLLS fitting -Aberration correction -Quantification using EELS -Ultrafast compositional mapping -High energy resolution chemical mapping and identification of different chemical phases -High energy resolution EELS STEM analysis -Atomic resolved level analysis using EELS -How to best optimize the spectrometer and increase the sensitivity -How to set up the microscope to get the best EELS results
This event is free to registered participants. Register online at: http://www.gatan.com/company/events/advanced-eels-and-eftem-workshop-harvard-university
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Title-Subject: [Filtered] staff scientist position
Message: Staff Scientist Position
The Laboratory of Cell Structure and Dynamics (LCSD) in the Division of Intramural Research (DIR), National Institute on Deafness and Other Communication Disorders (NIDCD), National Institutes of Health (NIH), seeks a Staff Scientist.
The LCSD seeks an integrated molecular understanding of the architecture, dynamics, function, and renewal of specialized cellular structures underlying the mechanosensory function of auditory and vestibular sensory hair cells. Current projects at the LCSD involve the use of a combination of cell biology approaches with super-resolution fluorescence microscopy and cryo-electron microscopy to examine the molecular architecture of the actin-based sensory stereocilia and their mechanotransduction molecular complex.
The NIDCD intramural program is comprised of a highly interactive and dynamic group of scientists at the forefront of research on communication disorders. The LCSD is located in the newly constructed, multi-disciplinary Porter Neuroscience Research Center on the main NIH campus in Bethesda, Maryland.
The LCSD Staff Scientist serves as a laboratory leader who contributes to mentoring of postdoctoral fellows and other laboratory staff and trainees. The position requires outstanding interpersonal skills, teamwork, and excellent oral and written communication abilities. The successful candidate should have a doctoral degree, postdoctoral research experience, and an outstanding publication record. Requisite expertise includes molecular and cellular biology, and either advanced fluorescence microscopy imaging or CryoEM. Salary is commensurate with experience.
Applicants should submit their curriculum vitae with bibliography, statement of interest, and names and contact information for three references to Bechara Kachar at kacharb-at-nidcd.nih.gov. Applications will be reviewed starting May 15, 2016, and accepted until the position is filled. HHS, NIH, and NIDCD are Equal Opportunity Employers. For more information about the LCSD and NIDCD, please visit our website: https://www.nidcd.nih.gov/about/staff/bechara-kachar-md https://www.nidcd.nih.gov/research/labs/section-structural-cell-biology
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Email: akgadde-at-mix.wvu.edu Name: akshitha
Organization: West Virginia University
Title-Subject: [Filtered] Image Processing
Message: Can someone help me understand in detail how the image processing works in background in a scanning electron microscope. How an image is produced from the current signal input to the image processing input in a scanning electron microscope.
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Announcing the Fall Biological Conference at the University of Illinois Materials Research Lab.
DATE: November 2-3, 2016
Conference Title: Diverse Fields of Study... Steps toward Medicine. ________________________________________ An apropos title, as UIUC is starting up its own medical school, MD/PHD with the PhD in Bioengineering.
Attendee registration to open in June and is open to scientists from all over. =========================================================
Vender registration is open NOW. ==========================
Student and Post Doc Presentation Divisions Competition: ============================================= up to 4 post docs, up to 9 students Register from June to October 1st, and upload 1/2 page abstract that will be judged by a panel for acceptance. Winners receive monetary awards, registration waved, and their image on our website for several months. Students as well as post docs from other institutions are welcome.
Invited Speakers: ==============
Our student competition: up to 4 post docs, up to 9 students.
Dr. Catherine Murphy, Chemistry, MRL associate director Gold Nanoparticles: Physics, Chemistry, Biology and Ecology
Adele Akhtar of Psionic, The Future of Upper Limb Prosthetic Devices, Doctoral Candidate Adele Akhtar owns a company that works with prosthetics that can sense touch pressure.
Dr. Ralph Nuzzo - Chemistry/ MRL about Hydrogel and cells
Dr Amy Wagoner - Johnson MechSci Biomaterials, bone implants
Dr Rashid Bashir - Bioengineering.
Dr. Mauro Sardela our new MRL director of the MRL Central Research Facilities, covering what our facility is all about.
Also a representative from our Safety department, topic to be determined.
VENDORS: ===========
This year we have 2 full days of conference,and we have some treats for our vendors. the second day. The vendor area will be open to the public, so this will give you a chance to set up demos and schedule tutorials at your table. Also, you can invite your contacts to come and visit you, regardless of if they have registered. So the second day is public and yours to add visitors, tutorials etc, as you may wish.
The second thing we would like to do for you is give you a chance to speak at the conference. When registering, you can upload a one page abstract to submit, images welcome. This should be in the form of a teaching tutorial. We were able to carve out some time, the 4 best panel judged abstracts will be able to give a 30 minute talk on the second day.
Abstract Deadline for the abstract submission is July 4th, 2016, and folks will know one way or the other by July 10th.
Registration deadline is closer to the meeting, but will end if all tables are full.
There is no fee for the chance to speak, and for 2 full days of tables, the price is $250/ table.
Registration is now open at: "MRL Biological Conference 2016 Exhibitor Registration" https://my.mrl.illinois.edu/eventreg/register.asp
Thanks! More information at the start of registration for attendees is to come.
Lou Ann
{ { { { { { { { { { { { { { { { {} } } } } } } } } } } } } } } } Lou Ann Miller Biological Electron Microscopy Frederick Seitz Materials Research Laboratory Lab Room 125 Office 374 MRL 104 South Goodwin Ave Urbana, IL 61801 Campus mail code: MC-230 Hours: 7am-3:30 pm
LaB6 users, I am not losing my touch and there is no LaB6 death vortex hanging over Ithaca! I have a whole slew of responses that concur with the issues I have been having. If any of you who are having the same issue please get in touch with me because if we don t tell the vendors and the suppliers that their product has changed for the worse they will still keep selling us garbage because either they don t know or just don t care. BTW, not one retort from Kimball or Denka yet.
I think all we want is a LaB6 that shows us a Maltese Cross, doesn t drift X-Y & Z to the point that the deflectors can t compensate (this is a common issue, you take the gun out and the tip still looks perfectly centered, you apply a current and it tilts toward the Emerald City), and a long life; just the way they used to be ten years ago.
I can t make it to M&M this year or next, that s when the ankle bracelet comes off, but this may be a good topic for a round table because we the users are not imagining things.
Send me some more horror stories, I will compile them and post them or you guys just throw them up on the Microscopy list, do not respond to Phil, I may owe him a keg if you reply to him.
jg
John L. Grazul {jlg98-at-cornell.edu}
==============================Original Headers============================== 7, 30 -- From oshel1pe-at-cmich.edu Thu Apr 21 13:09:47 2016 7, 30 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116]) 7, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u3LI9lt5003974 7, 30 -- for {microscopy-at-microscopy.com} ; Thu, 21 Apr 2016 13:09:47 -0500 7, 30 -- Received: from cas.cmich.edu (async2.cmich.edu [141.209.15.141] (may be forged)) 7, 30 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id u3LIBkX7008383 7, 30 -- for {microscopy-at-microscopy.com} ; Thu, 21 Apr 2016 14:11:47 -0400 7, 30 -- Received: from CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) by 7, 30 -- cas2.central.cmich.local (2002:8dd1:f8d::8dd1:f8d) with Microsoft SMTP Server 7, 30 -- (TLS) id 14.3.248.2; Thu, 21 Apr 2016 14:11:47 -0400 7, 30 -- Received: from bio-br024c-mac01.local (141.209.2.100) by mail.cmich.edu 7, 30 -- (141.209.15.90) with Microsoft SMTP Server (TLS) id 14.3.248.2; Thu, 21 Apr 7, 30 -- 2016 14:11:46 -0400 7, 30 -- Message-ID: {571917E2.1040407-at-cmich.edu} 7, 30 -- Date: Thu, 21 Apr 2016 14:11:46 -0400 7, 30 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 7, 30 -- Reply-To: {oshel1pe-at-cmich.edu} 7, 30 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 7, 30 -- MIME-Version: 1.0 7, 30 -- To: micro {microscopy-at-microscopy.com} 7, 30 -- Subject: Follow up to LaB6 Issues 7, 30 -- Content-Type: text/plain; charset="windows-1252"; format=flowed 7, 30 -- Content-Transfer-Encoding: 8bit 7, 30 -- X-Originating-IP: [141.209.2.100] 7, 30 -- X-Bayes-Prob: 0.9792 (Score 4, tokens from: -at--at-RPTN, default) 7, 30 -- X-Spam-Score: 2.80 (**) [Hold at 6.00] RDNS_NONE,_L_LEXNRL,_L_LLEXCH,SPF(softfail:0),RBL(rp-good:-0.1),Bayes(0.9792:4.0) 7, 30 -- X-CanIt-Geo: ip=141.209.15.141; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6 7, 30 -- X-CanItPRO-Stream: default 7, 30 -- X-Canit-Stats-ID: 05QJ6bLCS - d89b668dac04 - 20160421 7, 30 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.15.116 ==============================End of - Headers==============================
We too have had problems with Denka LaB6 on our FEI T20. Short life and bad drift. We have to take the wehnelt out several times to re-center the LaB6 as it runs out of gun tilt. We are experimenting with whether to just turn down the filament current for overnight or if it is best to turn it off completely. After a long spell of just turning it lower we are now turning it on in the morning and off at the end of the day. Its early days but I think we have better lifetime. Next time we change filament we have plans to try a different cathode assembly.
Chris
Christopher J. Gilpin Ph.D. Director, Life Science Microscopy Facility Purdue University Whistler Hall of Agriculture Research, Room S052 170 S. University St West Lafayette, IN 47907 765-494-7750 gilpin-at-purdue.edu lsmf-at-purdue.edu reaches everyone in the facility
-----Original Message----- X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu] Sent: Wednesday, April 20, 2016 4:19 PM To: Gilpin, Christopher J {gilpin-at-purdue.edu}
Note! Please do *not* use "Reply"! Send any response to the list or to John L. Grazul {jlg98-at-cornell.edu} . I'm sending this for a colleague locked behind a Cornell Curtain. Phil
Message: Fellow microscopists it has been a long time since I have posted mainly because anything that comes out of Cornell is un-postable for really good reasons I assume. The huge issue I have been having for the past couple of years is the quality, reliability and life span of my LaB6 sources. Now this is not an issue with just one scope or just one vendor. Tungsten filaments work fantastic and hit all the numbers perfectly in both of our 120kV Tecnais. When we put a LaB6 in really weird things happen. Before you get suspicious and think we just started up with LaB6, this is all that we have ever used on our tools, I just had to test the scopes with W to make sure I wasn't imagining things. We need LaB6 because of the brightness and hours of use; each scope is used 40-50 hr/week. So much for the introduction, now on to the issues:
1. I have not seen a Maltese cross on either a Kimball or Denka in 5 years or so, or about 5- 8 filaments, on either of my Tecnais. The engineers and I just undersaturate and go for max brightness. 2. Filament life used to be 2500-3000 hours, the last five tips have flamed out around 500 hours. BTW the vacuum has been rock steady in both scopes. 3. I have had two tips fail due to micro cracks in the carbon crucible. 4. The last five tips drift for the first 100-200 hours, and we are talking X-Y and Z. This is to the point that the deflectors can't compensate and I have to break vacuum and re-center manually.
5. My 24 Volt power supply for the filament is fine, swapped them around my scopes with the same result.
If the darn things didn't cost nearly $900.00 I might tolerate this. I have to give high marks to EMS, they have been listening to my complaints and have made good on the tips that have failed out of the box. But the quality of the product I really have questions about. Has Kimball changed their carbon supplier, is it softer? Why are there cracks? And the last question is have any of you guys had the same or similar issues? And the absolutely final question is am I missing something? After 30+ years of ripping scopes apart I might just have lost the magic touch or there is a vortex of Lab6 instability issues in Ithaca.
Please contact me on or off line: John L. Grazul {jlg98-at-cornell.edu} --
==============================Original Headers============================== 6, 33 -- From oshel1pe-at-cmich.edu Wed Apr 20 14:58:44 2016 6, 33 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116]) 6, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u3KJwiex003213 6, 33 -- for {microscopy-at-microscopy.com} ; Wed, 20 Apr 2016 14:58:44 -0500 6, 33 -- Received: from cas.cmich.edu ([141.209.15.90]) 6, 33 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id u3KK0dwT018896 6, 33 -- for {microscopy-at-microscopy.com} ; Wed, 20 Apr 2016 16:00:39 -0400 6, 33 -- Received: from cas4.central.cmich.local (2002:8dd1:f03::8dd1:f03) by 6, 33 -- CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) with Microsoft SMTP Server 6, 33 -- (TLS) id 14.3.248.2; Wed, 20 Apr 2016 16:00:39 -0400 6, 33 -- Received: from CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) by 6, 33 -- CAS4.central.cmich.local (2002:8dd1:f03::8dd1:f03) with Microsoft SMTP Server 6, 33 -- (TLS) id 14.3.248.2; Wed, 20 Apr 2016 16:00:39 -0400 6, 33 -- Received: from bio-br024c-mac01.local (141.209.2.100) by mail.cmich.edu 6, 33 -- (141.209.15.90) with Microsoft SMTP Server (TLS) id 14.3.248.2; Wed, 20 Apr 6, 33 -- 2016 16:00:39 -0400 6, 33 -- Message-ID: {5717DFE6.2060309-at-cmich.edu} 6, 33 -- Date: Wed, 20 Apr 2016 16:00:38 -0400 6, 33 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 6, 33 -- Reply-To: {oshel1pe-at-cmich.edu} 6, 33 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 6, 33 -- MIME-Version: 1.0 6, 33!
We to have been having the same problem with Denka LaB6 filaments on a JEM-3010. We have taken the last two out after aligning them because they appear to be tilted only to find that they are perfectly aligned!
Over time the last one got better and we got closer to the Maltese cross . We have not seen a sharp decline in lifetime however.
Regards
Alan
On 4/20/2016 3:00 PM, oshel1pe-at-cmich.edu wrote: ---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Note! Please do *not* use "Reply"! Send any response to the list or to John L. Grazul {jlg98-at-cornell.edu} . I'm sending this for a colleague locked behind a Cornell Curtain. Phil
Message: Fellow microscopists it has been a long time since I have posted mainly because anything that comes out of Cornell is un-postable for really good reasons I assume. The huge issue I have been having for the past couple of years is the quality, reliability and life span of my LaB6 sources. Now this is not an issue with just one scope or just one vendor. Tungsten filaments work fantastic and hit all the numbers perfectly in both of our 120kV Tecnais. When we put a LaB6 in really weird things happen. Before you get suspicious and think we just started up with LaB6, this is all that we have ever used on our tools, I just had to test the scopes with W to make sure I wasn t imagining things. We need LaB6 because of the brightness and hours of use; each scope is used 40-50 hr/week. So much for the introduction, now on to the issues:
1. I have not seen a Maltese cross on either a Kimball or Denka in 5 years or so, or about 5- 8 filaments, on either of my Tecnais. The engineers and I just undersaturate and go for max brightness. 2. Filament life used to be 2500-3000 hours, the last five tips have flamed out around 500 hours. BTW the vacuum has been rock steady in both scopes. 3. I have had two tips fail due to micro cracks in the carbon crucible. 4. The last five tips drift for the first 100-200 hours, and we are talking X-Y and Z. This is to the point that the deflectors can t compensate and I have to break vacuum and re-center manually.
5. My 24 Volt power supply for the filament is fine, swapped them around my scopes with the same result.
If the darn things didn t cost nearly $900.00 I might tolerate this. I have to give high marks to EMS, they have been listening to my complaints and have made good on the tips that have failed out of the box. But the quality of the product I really have questions about. Has Kimball changed their carbon supplier, is it softer? Why are there cracks? And the last question is have any of you guys had the same or similar issues? And the absolutely final question is am I missing something? After 30+ years of ripping scopes apart I might just have lost the magic touch or there is a vortex of Lab6 instability issues in Ithaca.
Please contact me on or off line: John L. Grazul {jlg98-at-cornell.edu}
-- Alan W Nicholls, PhD Associate Director, RRC Director of Research Service Facility (Electron Microscopy) Research Resources Center - East (M/C 337) Room 110, Science and Engineering South Building The University of Illinois at Chicago 845 West Taylor St Chicago, IL 60607-7058
Tel: 312 996 1227
Web site http://www.rrc.uic.edu/ems Wiki site https://wiki.rrc.uic.edu/wiki/EMS
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