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From: microscopy.listserver-at-gmail.com
Date: Fri, 1 Jan 2016 12:58:49 -0600
Subject: [Microscopy] Administrivia - Happy New Year 2016

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Happy New Year Colleagues;

Welcome to the another year of operation of the Microscopy ListServer
a free service to the world wide microscopy community, sponsored
jointly by your Friendly Neighborhood SysOp and the Microscopy Society
of America.

During 2015, the ListServer delivered 800+ messages to more than 4000 subscribers
around the world, with minimal hassels (that I know about). For those of you that are
statistics junkies this year you generated ~ 165+ Gbits of Email traffic and ~ 3.2 Million
Email messages were sent out this year by my tired and old little server.
Some time this year I will be migrating the Listserver to a new system, so
you might see a short outage.

As usual you don't want to know how much Junk Mail and spam has been filtered out.


The complete Microscopy ListServer Archives for 2015-1994 (~ are on-line at

http://www.microscopy.com.

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From: microscopy.listserver-at-gmail.com
Date: Mon, 4 Jan 2016 13:36:41 -0600
Subject: [Microscopy] viaWWW:Job Opening at Bruker Nano Analytics

Contents Retrieved from Microscopy Listserver Archives
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X-from: don.becker-at-bruker.com

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Email: don.becker-at-bruker.com
Name: Don Becker

Organization: Bruker Nano

Title-Subject: [Filtered] Job Opening at Bruker Nano Analytics

Message: Bruker Nano Analytics has an immediate opening for a Senior
Sales Representative for Microanalysis in the Southeastern United
States. The successful candidate will have a minimum of 3 -n 5 years
experience in the sale of scientific equipment, and will sell, market,
and support our microanalysis tools with new and existing customers, as
well as support our OEM sales channel partners. The sales territory
includes CO, NM, TX, OK, AL, NC, SC, FL, GA, LA, and MS.

A complete job description and application can be found at:

https://englishcareers-bruker.icims.com/jobs/3176/sr.-sales-representative---microanalysis/job?mobile=false&width=940&height=500&bga=true&needsRedirect=false&jan1offset=-300&jun1offset=-240


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From: wsalmon-at-wi.mit.edu
Date: Wed, 6 Jan 2016 15:42:09 -0600
Subject: [Microscopy] LM - 2016 Analytical and Quantitative Light Microscopy Course @ MBL

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues,

We are excited to announce this year's run of Analytical and Quantitative Light Microscopy, held at the Marine Biological Laboratory, Woods Hole, MA, USA. The course will run May 4 - May 13 , 2016.

AQLM is a comprehensive and intensive course in light microscopy for researchers in biology, medicine, and material sciences. This course provides a systematic and in-depth examination of the theory of image formation and application of digital methods for exploring subtle interactions between light and the specimen. This course emphasizes the quantitative issues that are critical to the proper interpretation of images obtained with modern wide-field and confocal microscopes.

Laboratory exercises, demonstrations, and discussions include:
* geometrical and physical optics of microscope image formation including Abbe's theory of the microscope and Fourier optics;
* phase contrast, polarization and interference microscopy;
* fluorescence microscopy, quantification of fluorescence, and fluorescent proteins;
* principles and application of digital imaging and quantitative digital image deconvolution;
* digital image processing and object identification and tracking;
* live cell and ratiometric imaging for FRET and ion concentration imaging;
* confocal microscopy and specialized methods such as TIRF and FLIM; and
* new advances in light microscopy such as FCS, PALM, STORM, SIM and SPIM.

The course web site is at http://www.mbl.edu/aqlm .

Within the course, we often refer to AQLM as "Microscopy Boot Camp". We cover every topic with a lecture and an in-depth hands-on lab. It's intense, it's hardcore, and it's really fun.

Why not join us for AQLM (or recommend one of your lab members or core facility users)? You'll never forget it.

Applications due January 25 , 2016.

D irectors: Jagesh Shah (Harvard Medical School), Justin Taraska (National Institutes of Health)
Course Laboratory Director: Wendy Salmon (Whitehead Institute/MIT)

Cheers,
Jagesh, Justin and Wendy

Posters available at http://www.mbl.edu/education/files/2014/04/AQLM_POSTER_2016v1.pdf

~~~~~~~~~~~~~~~~~~~~~~~
Wendy Salmon
Light Microscopy Specialist
Whitehead Institute for Biomedical Research
W.M. Keck Imaging Facility
9 Cambridge Center, Rm 447
Cambridge, MA 02142
c: 617-429-0158
e: wsalmon-at-wi.mit.edu
w: http://staffa.wi.mit.edu/microscopy/

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From: benada-at-biomed.cas.cz
Date: Thu, 7 Jan 2016 08:45:06 -0600
Subject: [Microscopy] MegaViewII Trouble

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello All,
We have a trouble with our old MegaViewII TEM camera. Just before the
end of the last year, the power source of a PC we are using with
MegaViewII camera has gone. Just now we replaced the power source with
a new one. All seemed to be OK but when we tried to record the image
with MegaViewII camera we got a strange result. The image can be seen
on this link:

http://www2.biomed.cas.cz/~benada/MegaViewII_trouble.html


Please does anybody had to solve such problem?

Thanking for any response in advance.
Oldrich

--
Oldrich Benada
Institute of Microbiology CAS, v.v.i.
Videnska 1083
142 20 Prague 4
Czech Republic


Upozorneni:
Neni-li v teto zprave vyslovne uvedeno jinak, ma tato E-mailova zprava nebo jeji prilohy pouze informativni charakter. Tato zprava ani jeji prilohy v zadnem ohledu ustavy AV CR, v.v.i. k nicemu nezavazuji. Text teto zpravy nebo jejich priloh neni navrhem na uzavreni smlouvy, ani prijetim pripadneho navrhu na uzavreni smlouvy, ani jinym pravnim jednanim smerujicim k uzavreni jakekoliv smlouvy a nezaklada predsmluvni odpovednost ustavu AV CR, v.v.i.

Disclaimer:
If not expressly stated otherwise, this e-mail message (including any attached files) is intended purely for informational purposes and does not represent a binding agreement on the part of Institutes of AS CR. The text of this message and its attachments cannot be considered as a proposal to conclude a contract, neither the acceptance of a proposal to conclude a contract, nor any other legal act leading to concluding any contract; nor it does not create any pre-contractual liability on the part of Institutes of AS CR.


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10, 42 -- From: Oldrich Benada {benada-at-biomed.cas.cz}
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From: microscopy.listserver-at-gmail.com
Date: Thu, 7 Jan 2016 14:55:18 -0600
Subject: [Microscopy] viaWWW: TEM Glove box sample transfer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: mlibbee-at-lbl.gov

This Question/Comment was submitted to the Microscopy Listserver
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Email: mlibbee-at-lbl.gov
Name: Marissa

Organization: LBL

Title-Subject: [Filtered] Glove box

Message: To those of you who transfer samples to a TEM holder in a glove box, will you please
contact me offline with the name of the glove box supplier?

Thanks!

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From: benada-at-biomed.cas.cz
Date: Fri, 8 Jan 2016 02:13:04 -0600
Subject: [Microscopy] Re: MegaViewII Trouble

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Hello Warren, Ravi and Larry,
Thank you for your comments and suggestions. Yesterday I forgot one
important think, the pattern (image) that we are getting from
megaViewII does not depend on electron beam intensity. Even when the
beam is switched off, we get this pattern (image).

Best regards Oldrich

On Thu, 7 Jan 2016 15:58:40 +0000, "Straszheim, Warren E [BIOTC]"
{wesaia-at-iastate.edu} wrote :
} It looks like the synchronization has gone out. It is like what I see
} on our SEM when the raster on the sample doesn't match the raster on
} the screen.
}
} I presume the system is operating in TEM mode and that the MegaView
} is a CCD camera. If so, the only way that such a phenomenon might
} occur is if the number of pixels per line is not matching between the
} CCD and display. It seems the number of pixels across your image is
} slightly less than the number being read across the CCD. Therefore,
} each successive line is shifted some to the right.
}
} Is the sample a nice simple structure? I would wonder what a single
} vertical grid bar would look like.
}
} Warren
}
} -----Original Message-----
} From: benada-at-biomed.cas.cz [mailto:benada-at-biomed.cas.cz]
} Sent: Thursday, January 07, 2016 8:48 AM
} To: Straszheim, Warren E [BIOTC]
} Subject: [Microscopy] MegaViewII Trouble
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
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}
} Hello All,
} We have a trouble with our old MegaViewII TEM camera. Just before the
} end of the last year, the power source of a PC we are using with
} MegaViewII camera has gone. Just now we replaced the power source with
} a new one. All seemed to be OK but when we tried to record the image
} with MegaViewII camera we got a strange result. The image can be seen
} on this link:
}
} http://www2.biomed.cas.cz/~benada/MegaViewII_trouble.html
}
}
} Please does anybody had to solve such problem?
}
} Thanking for any response in advance.
} Oldrich
}
} --
} Oldrich Benada
} Institute of Microbiology CAS, v.v.i.
} Videnska 1083
} 142 20 Prague 4
} Czech Republic


Upozorneni:
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From: mdelann1-at-jhmi.edu
Date: Fri, 8 Jan 2016 10:38:14 -0600
Subject: [Microscopy] Stabilizing pili on bacteria for SEM

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Name: Gary Bloomer

Organization: Montana State University

Title-Subject: [Filtered] Chromophores characterized for 2-photon microscopy

Message: We are doing 2-photon microscopy but the available chromophores all seem to be
characterized only for 1 photon fluorescence. Setting parameters on the light source according to
this data may be creating an error in our results. Are others concerned with this and are there
solutions such as look-up tables available to properly set laser parameters for available dyes?

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From hanja07656515151viu-at-gmail.com Fri Jan 8 08:09:14 2016
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Message-ID: {C2B18D2B.24421114-at-gmail.com}

Dear Listers,

I'm currently fixing a strain of E. Coli with pili with 2%GA , 2%Paraform. In .1M Sorensen's phosphate buffer. Filtering them through a small Millipore filter and then processing this filter disc in the usual way up through CPD, mounting, sputter coating with gold/palladium for SEM. Most of the pili seem to have fallen off the bacteria, plenty of loose pili on the filter beside the bacteria.

Couple of questions for everybody. First, can fixation cause pili to shear off the bacteria? Second, anyone have any suggestions on how to keep the pili attached to the bacteria for SEM observation? Thanks in advance, all help is appreciated.

Tom Bargar
UNMC
Electron Microscopy Core Facility
986395 Nebraska Medical Center
Omaha, NE 68198-6395
402-559-7347
tbargar-at-unmc.edu


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8, 37 -- From tbargar-at-unmc.edu Fri Jan 8 08:26:56 2016
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From advertise.bz222uuv-at-gmail.com Fri Jan 8 09:32:23 2016
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Message-ID: {62EBFA9A.D09DB7CA-at-gmail.com}

Tom,
Are you following the primary fixative with osmium? Does this strain have
pili? You can run a quick negative stain prep to confirm pili.
Also maybe some tannic acid in the primary fixative along with PF and Ga. I
never heard of pili falling of during fixation.

Good luck,
Michael Delannoy

-----Original Message-----
X-from: tbargar-at-unmc.edu [mailto:tbargar-at-unmc.edu]
Sent: Friday, January 08, 2016 9:38 AM
To: delannoy-at-jhmi.edu

Dear Listers,

I'm currently fixing a strain of E. Coli with pili with 2%GA , 2%Paraform.
In .1M Sorensen's phosphate buffer. Filtering them through a small
Millipore filter and then processing this filter disc in the usual way up
through CPD, mounting, sputter coating with gold/palladium for SEM. Most of
the pili seem to have fallen off the bacteria, plenty of loose pili on the
filter beside the bacteria.

Couple of questions for everybody. First, can fixation cause pili to shear
off the bacteria? Second, anyone have any suggestions on how to keep the
pili attached to the bacteria for SEM observation? Thanks in advance, all
help is appreciated.

Tom Bargar
UNMC
Electron Microscopy Core Facility
986395 Nebraska Medical Center
Omaha, NE 68198-6395
402-559-7347
tbargar-at-unmc.edu


The information in this e-mail may be privileged and confidential, intended
only for the use of the addressee(s) above. Any unauthorized use or
disclosure of this information is prohibited. If you have received this
e-mail by mistake, please delete it and immediately contact the sender.



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From: benada-at-biomed.cas.cz
Date: Fri, 8 Jan 2016 11:05:37 -0600
Subject: [Microscopy] Re: Stabilizing pili on bacteria for SEM

Contents Retrieved from Microscopy Listserver Archives
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Hello Tom,
We are using E. coli in our practical courses of electron microscopy in
biology. We usually process E. coli samples for TEM and SEM in
parallel.

At first, do you really need a SEM for imaging E. coli pili?
TEM and negative staining is much more faster a you even do not need to
fix E. coli sample at all. In our hand 1% ammonium molybdate, pH6.8-7.0
plus 0.1% trehalose mixture works quite well for negative staining of
bacteria on glow-discharge treated formvar/carbon grids.

Our procedure for SEM:
1. Start with 0.5 ml of E. coli culture; optical density ranging from
0.4 to 0.6.
2. Fix it with 3% glutaraldehyde for half an hour at room
temperature, then continue the fixation at 4 oC overnight.
3. Wash the fixed cells well with phosphate or cacodylate buffer
(three times at least).
4. Make a moist chamber from Petri dish, wet filter paper and Parafilm.
5. Place some poly-l-lysine treated circular cover-slips on the
Parafilm in the moist chamber and put 100 ,Aei 200 uL of fixed cells in the
washing buffer onto the individual cover-slip. Whole cover-slip area
should be covered with liquid. Let sediment the cells onto the
cover-slips at 4 oC overnight.
6. Wash the cover-slips with ddH2O.
7. Dehydrate through alcohol series; CPD; ... .

With this procedure the pili should stay attached to bacteria.

Best regards
Oldrich

--
Oldrich Benada
Institute of Microbiology CAS, v.v.i.
Videnska 1083
142 20 Prague 4
Czech Republic


On Fri, 8 Jan 2016 08:31:14 -0600, tbargar-at-unmc.edu wrote :
}
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} Dear Listers,
}
} I'm currently fixing a strain of E. Coli with pili with 2%GA ,
} 2%Paraform. In .1M Sorensen's phosphate buffer. Filtering them
} through a small Millipore filter and then processing this filter disc
} in the usual way up through CPD, mounting, sputter coating with
} gold/palladium for SEM. Most of the pili seem to have fallen off the
} bacteria, plenty of loose pili on the filter beside the bacteria.
}
} Couple of questions for everybody. First, can fixation cause pili to
} shear off the bacteria? Second, anyone have any suggestions on how
} to keep the pili attached to the bacteria for SEM observation?
} Thanks in advance, all help is appreciated.
}
} Tom Bargar
} UNMC
} Electron Microscopy Core Facility
} 986395 Nebraska Medical Center
} Omaha, NE 68198-6395
} 402-559-7347
} tbargar-at-unmc.edu
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From: microwink-at-gmail.com
Date: Fri, 8 Jan 2016 12:22:17 -0600
Subject: [Microscopy] Job Opening: TEM Applications Specialist at Virginia Tech in

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Virginia Tech in Blacksburg, VA, USA has an opening for an
Applications Specialist in Scanning and Transmission Electron
Microscopy. This position will help support internal and external
users of the Virginia Tech National Center for Earth and Environmental
Nanotechnology Infrastructure (NCE- {=NI) under the direct supervision of
NCE- {=NI Associate Director for Instrumentation & Technical Development.
The successful candidate will have their office located within the
Nanoscale Characterization and Fabrication Laboratory (NCFL). The NCFL
houses advanced field emission scanning electron microscopes with EDS
and EBSD capabilities, several transmission electron microscopes with
EDS, tomography, HRTEM, cryoTEM, and other capabilities, and a suite
of powerful surface spectroscopy tools including magnetic sector SIMS,
XPS, and more. Multiple sample preparation laboratories facilitate the
preparation of samples for analysis using SEM, TEM, AFM, EBSD, and
other techniques.

The general duties associated with the TEM Applications Specialist position are:

(a) TEM instrument operation including imaging, diffraction, and spectroscopy.
(b) TEM data analysis support including image analysis, EDS
quantification, diffraction pattern indexing, and more.
(c) Conduct directed pilot projects and help co-author scientific publications.
(d) Sample preparation and management for both internal and external users.

Qualified candidates should have experience with electron microscopy,
x-ray spectroscopy, and electron diffraction techniques including nano
beam diffraction and convergent beam electron diffraction. Candidates
should be comfortable assisting users in the safe and effective
operation of the S/TEM instruments. Candidates should be able to
effectively communicate with faculty, staff and students from a
variety of technical and cultural backgrounds.

Scientist/engineers experienced in Earth systems science are
especially encouraged to apply. Note: the majority of samples are
expected to be poorly crystallized, polymorphous, naturally occurring
materials ranging from micro- to nanoscale.

To view a more detailed list of expected duties and the qualifications
required, visit:

https://listings.jobs.vt.edu/postings/62637

Applicants can apply to this position by visiting the above link and
clicking on the "Apply for this Job" link near the top of the page. If
the above link does not work, then you can apply by visiting
www.jobs.vt.edu and searching for posting number SR0150183.

Virginia Tech does not discriminate against employees, students, or
applicants on the basis of age, color, disability, gender, gender
identity, gender expression, national origin, political affiliation,
race, religion, sexual orientation, genetic information, veteran
status, or any other basis protected by law. For inquiries regarding
non-discrimination policies, contact the executive director for Equity
and Access at 540-231-8771 or Virginia Tech, North End Center, Suite
2300 (0318), 300 Turner St. NW, Blacksburg, VA 24061.


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From: dave-at-boeckeler.com
Date: Mon, 11 Jan 2016 13:55:41 -0600
Subject: [Microscopy] job opening at RMC-Boeckeler in Tucson, Arizona

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Title-Subject: [Filtered] Craic Microspectrophotometer Users

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From improve.alexa.rankslsuzn-at-gmail.com Sat Jan 9 13:10:08 2016
Return-Path: {improve.alexa.rankslsuzn-at-gmail.com}
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Message-ID: {F8A49867.6949C4DE-at-gmail.com}

Dear Microscopists,

We have an entry level opening in our Tucson
manufacturing facility suitable for an enthusiastic
energetic
recent graduate with good communication skills, to
join us as a full time EM tech in our applications
support lab.

Initial duties include:-

* Ultramicrotome QC - cut ultra thin sections &
evaluate in TEM + basic QC evaluation of sub
assemblies/components + submit reports &
documentation
* Glass Knife Maker test, calibration and QC reports
* Rotary microtome QC - cut semi-thin to thick
paraffin and materials science samples -
evaluate & report
* TEM maintenance

with opportunities for growth in the following areas:

* Applications support evaluating customer specimens
and advising on appropriate sample prep
techniques - includes
both life science and materials science disciplines
* Cryo Ultramicrotome test and evaluation
* Assist with workshops and product demonstrations
* Field support if applicant is willing and able to
travel
* Sales and Service

Please respond by email to info-at-boeckeler.com

--
Dave Roberts
Director-RMC Microscopy Products
RMC-Boeckeler
Boeckeler Instruments Inc
4650 S Butterfield Drive
Tucson, Arizona 85714
Tel: 520-745-0001
Fax: 520-745-0004
www.rmcproducts.com
Skype:dave.robertsrmc


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From: tina-at-pbrc.hawaii.edu
Date: Wed, 13 Jan 2016 12:31:52 -0600
Subject: [Microscopy] iPhone microscope

Contents Retrieved from Microscopy Listserver Archives
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My apologies for cross-posting...

Someone has asked me about microscope attachments for iPhone. I've seen
lots of ads, but I haven't looked closely at any of the products. Do any
of you have opinions about products that turn your iPhone or Android into
decent microscopes? I'm not sure if she wants to go with a stand and
slides, or just take nicely magnified images on the go. I think the
former...

Aloha,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************

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From: steven.spurgeon-at-pnnl.gov
Date: Wednesday, January 13, 2016 at 10:56 AM
Subject: [Microscopy] iPhone microscope

Contents Retrieved from Microscopy Listserver Archives
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Hello Tina,

Here at PNNL we,Aeove actually developed a low-cost (~$1) cellphone camera that magnifies up to 1000x, depending on the specification. Our lab has freely released the blueprints for 3D printing or you can order pre-manufactured lenses that clip onto pretty much any smartphone. We use them frequently in our demos to high school and middle school children and they work quite well once you get the hang of them.

Here,Aeos a link to more details: http://availabletechnologies.pnnl.gov/technology.asp?id=393

Feel free to contact me if you have any questions.

* The views and opinions expressed in this email are my own and do not necessarily reflect those of PNNL, the Battelle Memorial Institute, the United States Government, or any agency thereof.
______________________________________
Steven R. Spurgeon, Ph.D.
Postdoctoral Research Associate
Physical and Computational Sciences Directorate

Pacific Northwest National Laboratory
902 Battelle Boulevard
P.O. Box 999 MSIN:K8-87
Richland, WA 99352

Tel: +1-509-371-7123
steven.spurgeon-at-pnnl.gov
www.stevenspurgeon.com {http://www.stevenspurgeon.com/}
www.pnnl.gov {http://www.pnnl.gov/}




X-from: "tina-at-pbrc.hawaii.edu" {tina-at-pbrc.hawaii.edu}
Reply-To: "tina-at-pbrc.hawaii.edu" {tina-at-pbrc.hawaii.edu}

My apologies for cross-posting...

Someone has asked me about microscope attachments for iPhone. I've seen
lots of ads, but I haven't looked closely at any of the products. Do any
of you have opinions about products that turn your iPhone or Android into
decent microscopes? I'm not sure if she wants to go with a stand and
slides, or just take nicely magnified images on the go. I think the
former...

Aloha,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************

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22, 29 -- From prvs=813e0a7d3=steven.spurgeon-at-pnnl.gov Wed Jan 13 13:11:57 2016
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22, 29 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} ,
22, 29 -- "tina-at-pbrc.hawaii.edu" {tina-at-pbrc.hawaii.edu}
22, 29 -- Subject: Re: [Microscopy] iPhone microscope
22, 29 -- Thread-Topic: [Microscopy] iPhone microscope
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From: FB.Fans.Cheap-at-cdn.rongdexuan.cn
Date: 14 Jan 2016 07:16:19 +0200
Subject: re: Stable Fanpage FB Likes

Contents Retrieved from Microscopy Listserver Archives
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Hi Tina,
Though not as cheap as the PNNL design, some colleagues of mine have
used this inexpensive, hardware-store-materials-based home-built design
for outreach and liked it:
http://www.instructables.com/id/10-Smartphone-to-digital-microscope-conversion/
Tyler Harvey
University of Oregon

On 2016-01-13 11:29, steven.spurgeon-at-pnnl.gov wrote:
} ----------------------------------------------------------------------------
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} http://www.microscopy.com/MicroscopyListserver
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} ----------------------------------------------------------------------------
}
} Hello Tina,
}
} Here at PNNL we/c,C ,Ncve actually developed a low-cost (~$1) cellphone
} camera that magnifies up to 1000x, depending on the specification. Our
} lab has freely released the blueprints for 3D printing or you can
} order pre-manufactured lenses that clip onto pretty much any
} smartphone. We use them frequently in our demos to high school and
} middle school children and they work quite well once you get the hang
} of them.
}
} Here/c,C ,Ncs a link to more details:
} http://availabletechnologies.pnnl.gov/technology.asp?id=393
}
} Feel free to contact me if you have any questions.
}
} * The views and opinions expressed in this email are my own and do not
} necessarily reflect those of PNNL, the Battelle Memorial Institute,
} the United States Government, or any agency thereof.
} ______________________________________
} Steven R. Spurgeon, Ph.D.
} Postdoctoral Research Associate
} Physical and Computational Sciences Directorate
}
} Pacific Northwest National Laboratory
} 902 Battelle Boulevard
} P.O. Box 999 MSIN:K8-87
} Richland, WA 99352
}
} Tel: +1-509-371-7123
} steven.spurgeon-at-pnnl.gov
} www.stevenspurgeon.com {http://www.stevenspurgeon.com/}
} www.pnnl.gov {http://www.pnnl.gov/}
}
}
}
}
} X-from: "tina-at-pbrc.hawaii.edu" {tina-at-pbrc.hawaii.edu}
} Reply-To: "tina-at-pbrc.hawaii.edu" {tina-at-pbrc.hawaii.edu}
} Date: Wednesday, January 13, 2016 at 10:56 AM
} To: Steven Spurgeon {steven.spurgeon-at-pnnl.gov}
} Subject: [Microscopy] iPhone microscope
}
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
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} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} My apologies for cross-posting...
}
} Someone has asked me about microscope attachments for iPhone. I've seen
} lots of ads, but I haven't looked closely at any of the products. Do
} any
} of you have opinions about products that turn your iPhone or Android
} into
} decent microscopes? I'm not sure if she wants to go with a stand and
} slides, or just take nicely magnified images on the go. I think the
} former...
}
} Aloha,
} Tina
}
} ****************************************************************************
} * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu
} *
} * Biological Electron Microscope Facility * (808) 956-6251
} *
} * University of Hawaii at Manoa *
} http://www.pbrc.hawaii.edu/bemf*
} ****************************************************************************
}
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} 22, 29 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} ,
} 22, 29 -- "tina-at-pbrc.hawaii.edu" {tina-at-pbrc.hawaii.edu}
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From: microscopy.listserver-at-gmail.com
Date: Thu, 14 Jan 2016 18:59:32 -0600
Subject: [Microscopy] viaWWW:Help with Orius column defect

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Email: Phil.Ahrenkiel-at-sdsmt.edu
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Title-Subject: [Filtered] Orius column defect

Message: Does anybody know how to use the "Defects" box in the "Camera Configuration" dialog in DM
for an Orius? I have spent several hours getting remote help from Gatan, but nobody is able to make
that feature actually work. The format seems to be "c2098", for column 2098. We are using DM1.85.1535.

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From: microscopy.listserver-at-gmail.com
Date: Thu, 14 Jan 2016 19:00:18 -0600
Subject: [Microscopy] viaWWW:Liposome in skin tissue - Ultrastructure by TEM

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Name: Ravindra Thakkar

Title-Subject: [Filtered] Liposome in skin tissue - Ultrastructure by TEM

Message: Does anyone worked on visualizing liposomes in skin tissue by TEM.
Is there any special precautions/steps to consider for processing the tissue.?

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From: nizets2-at-yahoo.com
Date: Fri, 15 Jan 2016 02:22:13 -0600
Subject: [Microscopy] Stabilizing pili on bacteria for SEM

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Dear Tom,

Pili are extremely delicate, I would guess that the filtration step is the critical one in your protocol because it requires pressure.
In this regard, the protocol of Oldrich is much softer since the cells are sedimented.
We placed bacteria on a porous membrane (used for cell biology) and sucked the liquid from the bottom side with a filter.
The filters give charging in SEM and they tend to melt under the beam, so be careful with your electrons!
(some years ago we had porous membranes made of aluminium, they were perfect for the job but I think they are not produced anymore)

Regards
Stephane


--------------------------------------------
On Fri, 1/8/16, benada-at-biomed.cas.cz {benada-at-biomed.cas.cz} wrote:

Subject: [Microscopy] Re: Stabilizing pili on bacteria for SEM
To: nizets2-at-yahoo.com
Date: Friday, January 8, 2016, 6:13 PM




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Hello Tom,
We are using E. coli in our practical courses of electron
microscopy in
biology. We usually process E. coli samples for TEM and SEM
in
parallel.

At first, do you really need a SEM for imaging E. coli
pili?
TEM and negative staining is much more faster a you even do
not need to
fix E. coli sample at all. In our hand 1% ammonium
molybdate, pH6.8-7.0
plus 0.1% trehalose mixture works quite well for negative
staining of
bacteria on glow-discharge treated formvar/carbon grids.

Our procedure for SEM:
1. Start with 0.5 ml of E. coli culture; optical density
ranging from
0.4 to 0.6.
2. Fix it with 3% glutaraldehyde for half an hour at room
temperature, then continue the fixation at 4 oC overnight.
3. Wash the fixed cells well with phosphate or cacodylate
buffer
(three times at least).
4. Make a moist chamber from Petri dish, wet filter paper
and Parafilm.
5. Place some poly-l-lysine treated circular cover-slips on
the
Parafilm in the moist chamber and put 100 ,Aei 200 uL of
fixed cells in the
washing buffer onto the individual cover-slip. Whole
cover-slip area
should be covered with liquid. Let sediment the cells onto
the
cover-slips at 4 oC overnight.
6. Wash the cover-slips with ddH2O.
7. Dehydrate through alcohol series; CPD; ... .

With this procedure the pili should stay attached to
bacteria.

Best regards
Oldrich

--
Oldrich Benada
Institute of Microbiology CAS, v.v.i.
Videnska 1083
142 20 Prague 4
Czech Republic


On Fri, 8 Jan 2016 08:31:14 -0600, tbargar-at-unmc.edu
wrote :
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} Dear Listers,
}
} I'm currently fixing a strain of E. Coli with pili with
2%GA ,
} 2%Paraform. In .1M Sorensen's phosphate buffer.-*
Filtering them
} through a small Millipore filter and then processing
this filter disc
} in the usual way up through CPD, mounting, sputter
coating with
} gold/palladium for SEM.-* Most of the pili seem to
have fallen off the
} bacteria, plenty of loose pili on the filter beside the
bacteria.
}
} Couple of questions for everybody.-* First, can
fixation cause pili to
} shear off the bacteria?-*-*-*Second, anyone
have any suggestions on how
} to keep the pili attached to the bacteria for SEM
observation?
} Thanks in advance, all help is appreciated.
}
} Tom Bargar
} UNMC
} Electron Microscopy Core Facility
} 986395 Nebraska Medical Center
} Omaha, NE 68198-6395
} 402-559-7347
} tbargar-at-unmc.edu
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From: tina-at-pbrc.hawaii.edu
Date: Fri, 15 Jan 2016 18:20:47 -0600
Subject: [Microscopy] iPhone microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hahahaa, you guys are so funny. I got 17 replies to my query about turning
an iPhone into a microscope, and no two were the same. And most of them
were along the lines of "I saw this one on the Internet...".

If anyone is particularly interested, I can send a list of links or
forward some of the responses. Here is the one that the person who
requested feedback is looking at (and is not one of the ones any of you
mentioned):

http://thegadgetflow.com/portfolio/uhandy-microscope/

It holds a slide and I think has a light source.

Aloha,
Tina

} My apologies for cross-posting...
}
} Someone has asked me about microscope attachments for iPhone. I've seen
} lots of ads, but I haven't looked closely at any of the products. Do any
of
} you have opinions about products that turn your iPhone or Android into
} decent microscopes? I'm not sure if she wants to go with a stand and
} slides, or just take nicely magnified images on the go. I think the
} former...
}
} Aloha,
} Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************

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From: microscopy.listserver-at-gmail.com
Date: Sat, 16 Jan 2016 06:11:07 -0600
Subject: [Microscopy] viaWWW:EC Autoscan SEM - looking for service manual

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Email: Chrissi.Luede-at-gmail.com
Name: Christopher L,demann

Organization: RWTH Aachen, GERMANY

Title-Subject: [Filtered] ETEC Autoscan SEM - looking for service manual

Message: Hello everyone,

I rescently acquired a vintage ETEC Autoscan SEM (rebadged by Siemens) and intend to refurbish it as
a hobby project.

The instrument came with a basic user manual, but in order to put the electronics back into
operation, I am looking for a more detailed service manual (circuit diagrams, exploded drawings,
component names, etc.). I would be very grateful, if anyone could share a digitised version of their
documents.


And finally, two technical questions:
1. Is there a list of head sizes for the various hex sockets on the electron optical column? Are
they metric or imperial? I found a couple of small screws which seem to require something between
2.5...3mm or 1/8...3/32", nothing I would deem as standard...

2. Would you happen to have any documentation on the diffusion pump? I assume it is a Varian type
M2. Is is worthwhile to replace it with a turbo pump? I already have all the necessary equipment and
would only need to buy an ASA/CF adapter flange plus a vibration damper.

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From: microscopy.listserver-at-gmail.com
Date: Sat, 16 Jan 2016 06:12:03 -0600
Subject: [Microscopy] viaWWW:ehigh Microscopy School 2016

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Email: SLC6-at-Lehigh.edu
Name: Sharon Coe

Organization: Lehigh University

Title-Subject: [Filtered] Lehigh Microscopy School 2016

Message: Now accepting registrations for the 46th annual Lehigh Microscopy School which will be held
on the campus of Lehigh University, Bethlehem, PA, June 5-10, 2016. All courses, lecturers, and
instrument suppliers will be together for what promises to be a phenomenal week! Course offerings
include: SEM and X-ray Microanalysis -i Introduction to SEM and EDS for the New Operator -i Focused
Ion Beam Instrumentation and Applications -i Problem Solving: Interpretation and Analysis of
SEM/EDS/EBSD Data -i Quantitative X-ray Microanalysis: Problem Solving Using EDS and WDS Techniques -i
Scanning Transmission Electron Microscopy: From Fundamentals to Advanced Applications. Register
and pay in full by April 15 for an Early Bird Discount! Contact: Sharon Coe
(sharon.coe-at-Lehigh.edu or 610-758-5133). See www.lehigh.edu/microscopy for registration form,
prices, and details about courses, lecturers, and instrument suppliers.

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From: microscopy.listserver-at-gmail.com
Date: Mon, 18 Jan 2016 19:17:46 -0600
Subject: [Microscopy] viaWWW:Microanalysis Society Topical Conference,Electron-Probe

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Email: paul.carpenter.epma-at-gmail.com
Name: Paul Carpenter

Organization: MAS

Title-Subject: [Filtered] MAS Electron-Probe Microanalysis 2016 workshop

Message: Microanalysis Society Topical Conference
Electron-Probe Microanalysis 2016

May 16-19, 2016 at the University of Wisconsin-Madison, WI

http://www.microprobe.org/topical-conferences/epma-2016-1/epma-2016



Registration is now open
See website for full details
Abstract deadline February 15, 2016
MAS Early Career Scholar financial travel support

Four-day intensive MAS topical conference

User meetings with EPMA 2016 sponsors on Monday, May 16, 2016
Three-day intensive topical conference, Tues-Thurs May 17-19, 2016
- EPMA, SEM, WDS, EDS techniques

Tutorial on EPMA and SEM quantitative analysis

Topics with invited and contributed presentations

General electron-probe microanalysis: analysis, instrumentation, methods

Trace element EPMA: background methods, high-resolution EPMA

Advanced WDS and EDS techniques, Monte Carlo simulation, accuracy studies

Quantitative compositional mapping by EPMA

Cathodoluminescence

Presentations and product information from the microanalysis vendor community

Inexpensive housing options

Group meals and discussion

Problems identified by the microanalysis community
-- solutions provided by the vendor community


Contact Paul Carpenter for more information: paul.carpenter.epma-at-gmail.com


Paul Carpenter
Earth and Planetary Sciences CB1169
Washington University
1 Brookings Drive
Saint Louis, MO 63130
314-602-9697






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From: microscopy.listserver-at-gmail.com
Date: Tue, 19 Jan 2016 06:35:52 -0600
Subject: [Microscopy] viaWWW:Looking for EDS for RJ Lee PSEM

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Email: mk-at-seclab.tuwien.ac.at
Name: Markus Kammerstetter

Organization: Secure Systems Lab Vienna, Vienna University of Technology

Title-Subject: [Filtered] Looking for EDS for RJ Lee PSEM

Message: Hello,

in our lab we have an older model RJ Lee/Aspex PSEM Scanning Electron Microscope in use to image and
analyze microchips.
We deprocess those chips layer by layer but currently do not have the exact information of the
material compositions used in those layers. Since the deprocessing and material removal techniques
(i.e. wet etching) heavily depend on the materials, we have been looking for an EDS system for quite
a while but unfortunately do not really have a budget for it.

It would thus be great if anyone on this list could spare a surplus EDS that is no longer needed
(detector, control HW and possibly also the SW tool).
Ideally it would include light elements and be compatible with the Aspex/RJ Lee PSEM so that we
could use the PSEM's software. However, virtually any EDS would also be nice to have if we can make
it fit. Also just heavy elements are fine as this would still allow us to characterize Si, Cu, Al,
W, Au and other material compositions typically found in integrated circuits.

We have access to LN2 for cooling, we can typically do mechanical and electronics repairs on our own
and we could also mill a custom adapter to make it fit onto our PSEM port.


Thank you,
Markus

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From: microscopy.listserver-at-gmail.com
Date: Wed, 20 Jan 2016 06:09:46 -0600
Subject: [Microscopy] viaWWW: Inviting you to attend and participate in EBSD 2016

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Email: lnbrewer1-at-eng.ua.edu
Name: Luke Brewer

Organization: University of Alabama

Title-Subject: [Filtered] Inviting you to attend and participate in EBSD 2016

Message: Dear Colleagues,

I invite you to attend and participate in Electron Backscatter Diffraction 2016! EBSD 2016 will be
held at The University of Alabama from May 24-26, 2016.

EBSD 2016 will have a one-day tutorial session with both lectures and hands-on laboratory
demonstrations designed to introduce EBSD to students and professionals new to the technique. There
will also be more advanced tutorials for experienced users on topics such as precession electron
diffraction, high angular resolution EBSD, and transmission kikuchi diffraction.

Days 2-3 will have platform presentations and posters devoted to the latest in EBSD-technique
development and applications to materials science, geology and earth sciences, and materials
engineering.

Support will be available to encourage students to attend this meeting.

Registration is now open, and we are accepting abstracts on-line until March 1, 2016.

Full details on the meeting can be found at:
http://www.microbeamanalysis.org/topical-conferences/ebsd-2016/welcome

Please let me know if you have any questions, and we do hope to see you at EBSD 2016!

Luke Brewer

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From: john.robson-at-boehringer-ingelheim.com
Date: Wed, 20 Jan 2016 10:57:49 -0600
Subject: [Microscopy] Pre-cleaned Microscope Slide recommendation

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I am having problems finding pre-cleaned microscope slides that are actually clean. I've looked at recently purchased slides form 2 vendors but neither product is "clean" with easily visible particulates and splotchy thin films randomly covering slide surfaces. Is anyone else out there noticing that previously good sources are now "dirty". I am using Polarized and bright field illumination. I would appreciate a product recommendation if you currently have a high quality source.

Thanks In advance, John


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From: microscopy.listserver-at-gmail.com
Date: Thu, 21 Jan 2016 06:55:59 -0600
Subject: [Microscopy] viaWWW:Seeking a repairperson for a Sorvall MT7000

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Email: Katherine.Kocan-at-okstate.edu
Name: Katherine Kocan

Organization: OSU Center for Veterinary Health Sciences

Title-Subject: [Filtered] Seeking a repairperson for a Sorvall MT7000

Message: If anyone has the name and contact information for a person who could provide service for a
Sorvall MT-7000, please share this information with me.

Thanks very much.

Kathy Kocan

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From: microscopy.listserver-at-gmail.com
Date: Thu, 21 Jan 2016 18:44:16 -0600
Subject: [Microscopy] viaWWW:EDS detector window problem

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Email: wzhe-at-laurentian.ca
Name: William

Organization: Laurentian University

Title-Subject: [Filtered] EDS detector window problem

Message: Hello,

We currently had problem with EDS SDD detector , it couldn-it be cooling down and a -eFault-i message
for detector condition came out at the end. One recommendation is that is sign of detector window
blown or pin hole on it. Is somebody ever went through this type of issue and have ideas about

1. how to identify if it is a window problem?
2. is it a simple job to replace a defective window if you have parts, like people always do for WDS
detector on their own ? If not,
3. any service company offers repairing in north America you may recommend ?

Thanks so much in advance,

William


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From: wsalmon-at-wi.mit.edu
Date: Sat, 23 Jan 2016 12:33:49 -0600
Subject: [Microscopy] LM - 2016 Analytical and Quantitative Light Microscopy Course @ MBL

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Application Deadline Monday, January 25. Please spread the word.

Dear Colleagues,

We are excited to announce this year's run of Analytical and Quantitative Light Microscopy, held at the Marine Biological Laboratory, Woods Hole, MA, USA. The course will run May 4 - May 13 , 2016.

AQLM is a comprehensive and intensive course in light microscopy for researchers in biology, medicine, and material sciences. This course provides a systematic and in-depth examination of the theory of image formation and application of digital methods for exploring subtle interactions between light and the specimen. This course emphasizes the quantitative issues that are critical to the proper interpretation of images obtained with modern wide-field and confocal microscopes.

Laboratory exercises, demonstrations, and discussions include:
* geometrical and physical optics of microscope image formation including Abbe's theory of the microscope and Fourier optics;
* phase contrast, polarization and interference microscopy;
* fluorescence microscopy, quantification of fluorescence, and fluorescent proteins;
* principles and application of digital imaging and quantitative digital image deconvolution;
* digital image processing and object identification and tracking;
* live cell and ratiometric imaging for FRET and ion concentration imaging;
* confocal microscopy and specialized methods such as TIRF and FLIM; and
* new advances in light microscopy such as FCS, PALM, STORM, SIM and SPIM.

The course web site is at http://www.mbl.edu/aqlm .

Within the course, we often refer to AQLM as "Microscopy Boot Camp". We cover every topic with a lecture and an in-depth hands-on lab. It's intense, it's hardcore, and it's really fun.

Why not join us for AQLM (or recommend one of your lab members or core facility users)? You'll never forget it.

Applications due January 25 , 2016.

Directors: Jagesh Shah (Harvard Medical School), Justin Taraska (National Institutes of Health)
Course Laboratory Director: Wendy Salmon (Whitehead Institute/MIT)

Cheers,
Jagesh, Justin and Wendy

Posters available at http://www.mbl.edu/education/files/2014/04/AQLM_POSTER_2016v1.pdf

~~~~~~~~~~~~~~~~~~~~~~~
Wendy Salmon
Light Microscopy Specialist
Whitehead Institute for Biomedical Research
W.M. Keck Imaging Facility
9 Cambridge Center, Rm 447
Cambridge, MA 02142
c: 617-429-0158
e: wsalmon-at-wi.mit.edu
w: http://staffa.wi.mit.edu/microscopy/

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From: marksmsa-at-gmail.com
Date: Mon, 25 Jan 2016 07:56:49 -0600
Subject: [Microscopy]

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I would like to draw your attention to the call for nominations for
the Gjonnes Medal at
http://www.iucr.org/resources/commissions/electron-crystallography/gjonnes-medal

The Gj/PInnes Medal in Electron Crystallography is awarded every three
years and recognizes an outstanding contribution to the field of
electron crystallography. Nomination for the Gj/PInnes Medal is open to
scientists and engineers in all areas of electron crystallography
defined in the broadest context as the branch of science that uses
electron scattering and imaging to study the structure of matter.

Please forward as appropriate.


--
Professor Laurence Marks
Department of Materials Science and Engineering
Northwestern University
www.numis.northwestern.edu
Corrosion in 4D: MURI4D.numis.northwestern.edu
Co-Editor, Acta Cryst A
"Research is to see what everybody else has seen, and to think what
nobody else has thought"
Albert Szent-Gyorgi


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From: zackg-at-berkeley.edu
Date: Mon, 25 Jan 2016 20:53:59 -0600
Subject: [Microscopy] Stoichiometry Fitter app

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello all,

I wrote a software called stoichometry fitter for doing quick analyses of spectra acquired by EDS (or XRF).
Originally, this was just for my own use but it has evolved to the point where I think it may be useful for some of you. I have now made it open source and put it on GitHub for anyone who wants to use it or contribute to it.

As input it takes At%, Wt% or OxWt% values.

It has the ability to fit several phases, for example, if you have a pyroxene which you think is contaminated with a bit of FeS, you can fit against a pyroxene triangle + FeS, and it will give you the correct EnFsWo values after removing the appropriate amount of Fe for the FeS. It also has a freeform analysis capability for a few minerals I use, and I'm hoping others will contribute their own analyses as well so we can all use them. For example, if you tell it the spectrum is a pyroxene, then it will compute T, M1 and M2 sites for all the elements using the Morimoto 1988 reference. If you are handy with python and numpy then you can create your own analyses with a minimum of four lines of code.

Finally, it has a TEM k-factor + thickness correction capability if you input each element as counts instead of the usual At%/Wt%/OxWt%. This is very useful if you have a TEM, but it would also apply to some thin-film experiments in an SEM/EPMA.

I made a short overview video here:

https://berkeley.box.com/s/jxpfwh7gt8ixwfi7z6k1c67jslyd8c5r

And the project is here:

https://github.com/ZGainsforth/StoichiometryFitter

I hope this is helpful for some of you!

Cheers,

Zack

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From: microscopy.listserver-at-gmail.com
Date: Tue, 26 Jan 2016 06:51:29 -0600
Subject: [Microscopy] viaWWW: Gatan EELS & EFTEM Analysis Training School

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Email: jhyun-at-gatan.com
Name: John Hyun

Organization: Gatan, Inc.

Title-Subject: [Filtered] EELS & EFTEM Analysis Training School

Message:
April 12-15, 2016
Gatan R&D Headquarters
Pleasanton, CA, USA

This comprehensive hands-on training course reviews the basic theory and practice of EELS imaging
and analysis in the TEM, but its main emphasis is on practical techniques, optimum deployment of
EELS and EFTEM hardware and software systems, and advanced applications. Some prior experience with
EELS, EFTEM, and energy filter systems is recommended, as is a good familiarity with TEM/STEM
instrumentation and techniques. By the end of the course, participants can expect to know how best
to optimize the performance of their EELS hardware as well as their EELS and EFTEM experimental
setups in order to capture and extract the maximum amount of information from their TEM samples.
Most of the curriculum is devoted to live microscope and computer lab sessions.

Topics:

-iFundamentals of EELS and energy-filtered imaging in TEM
-iPrinciples of operation of EFTEM and EELS systems
-iOptimization of EFTEM and EELS data acquisition
-iQuantification of elemental composition
-iOther information provided by EFTEM/EELS and how best to extract it
-iUse of EELS signals to form maps of elemental and chemical composition
-iEFTEM and STEM EELS spectrum imaging techniques
-iIdentification of material phases via EELS fine structure mapping
-iApplications to biological and physical science specimens

Complete information and online registration is available at:
http://www.gatan.com/company/events/eels-eftem-analysis-training-school-april-2016



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From: microscopy.listserver-at-gmail.com
Date: Tue, 26 Jan 2016 06:52:24 -0600
Subject: [Microscopy] viaWWW: LEO440 CPU CMOS battery

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Email: david-at-electronmicro.com
Name: David Scharf

Organization: SCHARF MICROSCOPY

Title-Subject: [Filtered] LEO440 CPU CMOS battery

Message: Can anyone help? I am getting a message that my CMOS battery has failed upon booting up my
LEO440 SEM. I have looked everywhere I can think of and cannot find that battery to replace it. It
is NOT on the computer board for sure! Must be external somewhere. Anyone service this instrument
before and know where its located? No mention of its location in my service manuals either. Thanks
in advance for any help.

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From: microscopy.listserver-at-gmail.com
Date: Wed, 27 Jan 2016 07:26:51 -0600
Subject: [Microscopy] rviaWWW:TEM - Cryo-ultramicrotomy of rubber sections

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Email: badamianand-at-bfusa.com
Name: Anand Badami

Organization: Bridgestone Research

Title-Subject: [Filtered] TEM - Cryo-ultramicrotomy of rubber sections

Message: Can anyone recommend a technique for collecting rubber sections during cryo-ultramicrotomy
that enables the sections to lie flat (i.e. as wrinkle free as possible) on a TEM support grid? I
currently collect my sections off the back of my cryoknife using a sucrose droplet, but as the
sections warm coming out of the cryochamber on the droplet they contract and wrinkle before being
deposited on a TEM grid (with or without carbon coating). Chamber temperature is -120 C. Any ideas
are appreciated. Thank you.

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From: krassimir.bozhilov-at-ucr.edu
Date: Wed, 27 Jan 2016 10:16:53 -0600
Subject: [Microscopy] March 10th, 2016 symposium

Contents Retrieved from Microscopy Listserver Archives
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Symposium in Advanced Electron Microscopy in Nanostructure Research and Inauguration of the new Titan Themis 300 S/TEM.


We are glad to announce and invite you to a full-day symposium in iAdvanced Electron Microscopy in Nanostructure Researchi and the inauguration of the new Titan Themis 300 S/TEM and the Quanta 200 duo beam instrument at the Central Facility for Advanced Microscopy and Microanalysis (CFAMM).

The inauguration will take place on March 10th, 2016 starting at 9 a.m. in 355 HUB building at the University of California at Riverside. Plenary seminar talks will be presented by leading researchers demonstrating the application of S/TEM and duo-beam techniques in nanostructure research. The plenary session will be followed by demo sessions on the instruments in the afternoon.

Full program announcement to follow soon.

To register contact vcredadmin-at-ucr.edu.

//////////////////////////////////////////////////////////////////////////
Krassimir Bozhilov,

Central Facility for Advanced Microscopy and Microanalysis,
Research & Economic Development Office
Materials Science and Engineering - BCOE
University of California
Riverside, CA 92521

ph. 951 827 2998
fax 951 827 2489
bozhilov-at-ucr.edu
/////////////////////////////////////////////////////////////////////




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From: microscopy.listserver-at-gmail.com
Date: Wed, 27 Jan 2016 19:37:37 -0600
Subject: [Microscopy] viaWWW:TEM & SEM of intestinal cells

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Email: wtkiii-at-hotmail.com
Name: WILLIAM KING

Title-Subject: [Filtered] TEM & SEM of intestinal cells

Message: I would like to know why the antigen behind Ulcerative Colitis has not been identified.
If the affected tissue were available, how many micrographs would it take to provide some visual cues?

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From: microscopy.listserver-at-gmail.com
Date: Wed, 27 Jan 2016 19:38:44 -0600
Subject: [Microscopy] viaWWW:MSNO 2016 Winter meeting

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Email: nxa157-at-case.edu
Name: Nanthawan Avishai

Organization: Microscopy Society of Northeastern Ohio, MSNO

Title-Subject: [Filtered] MSNO 2016 Winter meeting

Message: Our MSNO Winter Meeting 2016 will go to a new and exciting place: Lorain County Community
College. The meeting will be on March 2nd, 2016 3-7:15 pm. Please see the full program from attached
below.

The event will combine professional networking, lectures on by leading expert, guided tours & demos
at the impressive LCCC Desich Center, student posters, exhibitions by leading vendors in the field,
a nice dinner, a fun raffle and much more. Here are some of the highlights:
- Two main talks on Applications for MEMS Sensors (Matthew Apanius, Managing Director, SMART
Microsystems, Ltd) and "Basic Digital Imaging and Image Form" (by Dr. W. Gray Jerome, Vanderbilt
University Medical Center, a National MSA tour speaker).

- Tour at the Desich Center

The registration and more detail of this meeting can be found at
http://www.msneo.org/2016-winter-meeting.html

Looking forward to seeing you at LCCC!
MSNO Board

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From: parishcm-at-ornl.gov
Date: Thu, 28 Jan 2016 06:52:27 -0600
Subject: [Microscopy] FEI SuperX / Bruker: Strange performance at very low count rates

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers,

One or two of my users have observed a strange behavior on our FEI Talos F200X (FEI SuperX detector, Bruker pulse processor, Bruker software).

I'm not going to call this an "error" or a "fault," just something strange. Hopefully the list's hive mind can reality check me.

Let's say that on a very thin, low-Z sample, 400 pA might give 400 counts / sec on each of the four detectors (as seen in the TIA SuperX tab). 300 pA might give 300 cps. 200 pA might give zero. (Numbers are approximate.)

I interpret this to mean that there is a low-end discriminator on the pulse processor or software somewhere, and below a certain count rate no counts are registering. Is this possible? Is there a setting I can drill down to in order to let my users push to lower beam currents on their beam-sensitive samples?

This isn't a common problem, most of my Talos users are running ~5 nA, ~200 kcps beautifully, but I want to help all my users, not just those who can use the STEM as an electron sledgehammer.

Thanks in advance
Chad Parish

---------------------
Chad M. Parish, Ph.D.
Research and Development Staff Member
Radiation Effects and Microstructural Analysis Group
Materials Science and Technology Division
Oak Ridge National Laboratory
Phone: 1 865 574 0092
Email: parishcm-at-ornl.gov



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10, 53 -- Subject: FEI SuperX / Bruker: Strange performance at very low count rates
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From: CGorman-at-hookecollege.com
Date: Thu, 28 Jan 2016 07:51:27 -0600
Subject: [Microscopy] Short Course Announcement: SEM

Contents Retrieved from Microscopy Listserver Archives
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Greetings Microscopists,

The Hooke College of Applied Sciences, located in Westmont, IL, is offering its scanning electron microscopy short course March 14-18, 2016.*

In addition to lectures, this course emphasizes hands-on training using state-of-the-art equipment.* For further SEM training details and registration information, please follow the link below:

https://www.mccrone.com/scanning-electron-microscopy-course


Best regards-
__________________________________________________
Chris Gorman
Admissions Specialist
Hooke College of Applied Sciences
850 Pasquinelli Drive
Westmont, IL 60559-5539 USA
630-887-7100 (tel)
630-887-7412(fax)
www.hookecollege.com




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From: rok210-at-lehigh.edu
Date: Thu, 28 Jan 2016 08:22:20 -0600
Subject: [Microscopy] Re: FEI SuperX / Bruker: Strange performance at very low

Contents Retrieved from Microscopy Listserver Archives
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Hi Chad,

we have a JEOL X-ray detector with a Thermo Pulse-processor and software
and we can go way down in beam current to still collect X-rays. On a
recent trip our engineer showed me a software switch to remove the
"noise" peak counts from the spectrum and the reported count rate.

But if you are literally not seeing any peaks with 200pA then there's a
fundamental problem - call Bruker at once.

Good luck.

Rob Keyse

On 1/28/2016 8:12 AM, parishcm-at-ornl.gov wrote:
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} Dear Listers,
}
} One or two of my users have observed a strange behavior on our FEI Talos F200X (FEI SuperX detector, Bruker pulse processor, Bruker software).
}
} I'm not going to call this an "error" or a "fault," just something strange. Hopefully the list's hive mind can reality check me.
}
} Let's say that on a very thin, low-Z sample, 400 pA might give 400 counts / sec on each of the four detectors (as seen in the TIA SuperX tab). 300 pA might give 300 cps. 200 pA might give zero. (Numbers are approximate.)
}
} I interpret this to mean that there is a low-end discriminator on the pulse processor or software somewhere, and below a certain count rate no counts are registering. Is this possible? Is there a setting I can drill down to in order to let my users push to lower beam currents on their beam-sensitive samples?
}
} This isn't a common problem, most of my Talos users are running ~5 nA, ~200 kcps beautifully, but I want to help all my users, not just those who can use the STEM as an electron sledgehammer.
}
} Thanks in advance
} Chad Parish
}
} ---------------------
} Chad M. Parish, Ph.D.
} Research and Development Staff Member
} Radiation Effects and Microstructural Analysis Group
} Materials Science and Technology Division
} Oak Ridge National Laboratory
} Phone: 1 865 574 0092
} Email: parishcm-at-ornl.gov
}
}
}
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} 10, 53 -- Subject: FEI SuperX / Bruker: Strange performance at very low count rates
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--
Robert Keyse, DPhil
Research Scientist
Lehigh University

5, East Packer Avenue,
Whitaker Laboratory
Bethlehem,
PA 18015-3194

Office (610)758-3465
Fax (610)758-4244


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From: zaluzec-at-microscopy.com
Date: Thu, 28 Jan 2016 22:13:25 -0600
Subject: [Microscopy] viaWWW:FIB Technician Opening

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Name: Ming Zhang

Organization: Applied Materials Company

Title-Subject: [Filtered] FIB Technician Opening

Message: The successful candidate will focus on TEM sample preparations
by hands-on operations of ex-situ and in-situ lift-out systems.

Term:
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Other Skills:
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customers.
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From: microscopy.listserver-at-gmail.com
Date: Fri, 29 Jan 2016 19:42:54 -0600
Subject: [Microscopy] viaWWW:TEM: agar embedding cells

Contents Retrieved from Microscopy Listserver Archives
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Long ago, when histology was in vogue, I had a problem during the summer producing paraffin sections of good repute when the windows were full open, the temp was above 80, humidity was high and Summer was the bane of my progress.

If you have a fancy microtome that has a means of changing the angle of the knife, then you can refine the method that I used with a self-constructed series of thin, plywood templates that would permit me to change the setting of my blade as the weather moved thru its cycle. My goal, when the change was in the wind, was to produce a section whose height was greater than all the others I could produce with angles above and below the one that worked the best.

Air conditioning had just arrived, but not in our lab, so, I was stuck with my templates. Since I was working in a building with large windows, no cooling but the breeze on the mountain, patience and changing angles and, oh yes, changing the hardness of the paraffin as the weather went thru its yearly cycle. If one moved South to Florida to cut sections, one would have to fabricate a Florida paraffin mixture as an embedment - much too hard for even Spring or Fall in Bethlehem, PA.

So, we come to the advice. The first problem is compression. The second is the sharpness of the blade. The third is the vibrations that emanate from the microtome that can, in the first approximation, be reduced with a few drops of lubricant. Then, there is the vibration of the floor, the building, and the planet, and the solidity of the blade in its holder, the geometry of the blade and the angles of the cutting wedge on the edge of the blade. Sorry, I digress.

The optimal angle lies within a small range of not-so-optimal angles that are dependent on the hardness of the paraffin or plastic, the temp of the environment, the geometry of the cutting edge, the humidity, and everything else.

As I recall, my instructor, who had a PhD from Harvard under Alfred Romer, instructed me on the microtome and its personalities by advising me to, "fiddle with the block, the knife, the angle of the knife, the rate of the cutting cycle, and everything else until you get good sections." He ended with, "That's how I always did it."

Now that I've said and reviewed all of this I conclude that I have not been very helpful. That is primarily due to the fact that I haven't cut a section from a paraffin block since 1992, when I taught my younger daughter to cut sections for my research. As I recall, I used the word "fiddle" more than once during the tutorial, but again that was then and we sharpened our knives ourselves. She, by the way, was the best, and happiest 'cranker' I ever trained, and I convinced a small army to be good at it.

I hope that at the very least you are happy that you are younger, and don't have to do your sectioning in a room with the windows open - even in Winter!

Seicerely,

Fred Monson (he works on electron microscopes, hardly ever has to cut sections. and is contentedly moving toward 77 with good health and a lively sense of humor.

P.S. The adjustment of the knife angle is similar to the problem a baseball player has with his bat. He fiddles with everything to reduce the frequency of his pop ups. Neither are like the violin on which you are guaranteed that you will be world famous if you practice for 10,000 hours - either for the best or the worst noises you can project from the instrument.

Frederick C Monson, PhD
Center for Microanalysis and Imaging, Research and Training (CMIRT)
West Chester University of Pannsylvania
West Chester, PA, 1938
610-738-0437
fmonson-at-wcupa.edu
________________________________________
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Email: sbarlow-at-mail.sdsu.edu
Name: Steve Barlow

Organization: SDSU EM Facility

Title-Subject: [Filtered] TEM: agar embedding cells

Message: Some advice please on colorizing agar for embedding cells. In
the past, my osmicated pellets in agar blocks were easily trackable, but
when the cells are in a thin layer the cells don't give much contrast,
especially if not osmicated. I am collecting naked eukaryotic cells in
suspension culture, but have problems bring down the smaller cells by
centrifugation. Cultures containing cells are fixed in aldehyde or
aldehyde/osmium cocktail, collected onto millipore Nucleopore filters,
then held in place by adding warm agar over them on the filter.

When cooled, I peel off the filter, leaving me with a thin layer of
cells embedded in the agar. I then cut the agar layer into small blocks
to enhance fluid exchange during subsequent treatments. My problem is,
the cells are light colored to faintly black, and the thin layer of
cells does not stand out well, particularly when in the various stages
of epon infiltration/polymerization. I tried adding some azure II blue
dye I had on hand to the agar so I could see the agar blocks more
easily, but by the time I got up to epon/acetone, the color was gone.
Any suggestions for a dye that will withstand both the water based and
acetone solutions, or an alternative way to mark the little agar blocks
so I can more easily monitor them throughout the process?

Thanks in advance

Steve

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From: microscopy.listserver-at-gmail.com
Date: Fri, 29 Jan 2016 19:43:53 -0600
Subject: [Microscopy] viaWWW:Senior Scientist, Microscopy & Microanalysis - position

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Email: vicenzie-at-si.edu
Name: Ed Vicenzi

Organization: Smithsonian Institution

Title-Subject: [Filtered] Senior Scientist, Microscopy & Microanalysis -
position available

Message: [16-LM-CR] Senior Scientist, Microscopy & Microanalysis. A Sr.
Microscopist will characterize widely diverse samples, including organic
and inorganic chemicals, household goods, pharmaceuticals, foods,
plastics, etc. Analyst must execute experimental studies and deliver
high quality data using a broad range of analytical methods including
SEM, EDX, FTIR microscopy, Polarized Light microscopy, Fluorescence,
Image analysis. Proficiency with sample preparation methods
(sectioning, mounting microtome, etc.) required. Familiarity with
GMP/GLP and excellent writing skills. PhD in Analytical Chemistry - 3+
years industrial experience, M.S. -n 5+ years industrial experience.
Background in Mineralogy a plus

Please direct questions regarding this position to:

========================
Jonathan Chun, Ph.D.
Alliance Technologies
9 Deer Park Dr. Suite B
Monmouth Jct., NJ 08852
732-355-1234 (ph)
jchun-at-alliancetechgroup.com
www.alliancetechgroup.com

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From: microscopy.listserver-at-gmail.com
Date: Mon, 1 Feb 2016 13:53:29 -0600
Subject: [Microscopy] viaWWW:TEM FEI low background double tilt holder tip repair

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Email: 13qw9-at-queensu.ca
Name: Jason

Organization: Queen's University

Title-Subject: [Filtered] TEM FEI low background double tilt holder tip
repair

Message: Hi there,

We are encountering an issue with our FEI low background double tilt
holder. Probably because of some deformation on the holder tip, now the
two small pins cannot hold the sample cradle in position. Does anyone
have any experiences in fixing similar holders or know any company that
I can contact?

Thanks,

One colleague (Fei) has tried to send the same message with no luck. Not
sure what's the reason. Apologizes for letting you receive it twice.



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From: baskin-at-bio.umass.edu
Date: Mon, 1 Feb 2016 16:40:07 -0600
Subject: [Microscopy] Re: viaWWW:TEM: agar embedding cells

Contents Retrieved from Microscopy Listserver Archives
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Hi,
I have had luck with fast green added at the 100% ethanol step. I
make a saturated fast green solution in ethanol and add a drop or two at
the 100% ethanol step. It kept the agar blocks rather colored through
subsquent infiltrations and polymerization. Sorry I don't have handy
what "saturated" means in terms of mg/mL but if you are interested I
could probably dig that up somewhere.
Good luck,
Tobias

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}
} Title-Subject: [Filtered] TEM: agar embedding cells
}
} Message: Some advice please on colorizing agar for embedding cells. In
} the past, my osmicated pellets in agar blocks were easily trackable, but
} when the cells are in a thin layer the cells don't give much contrast,
} especially if not osmicated. I am collecting naked eukaryotic cells in
} suspension culture, but have problems bring down the smaller cells by
} centrifugation. Cultures containing cells are fixed in aldehyde or
} aldehyde/osmium cocktail, collected onto millipore Nucleopore filters,
} then held in place by adding warm agar over them on the filter.
}
} When cooled, I peel off the filter, leaving me with a thin layer of
} cells embedded in the agar. I then cut the agar layer into small blocks
} to enhance fluid exchange during subsequent treatments. My problem is,
} the cells are light colored to faintly black, and the thin layer of
} cells does not stand out well, particularly when in the various stages
} of epon infiltration/polymerization. I tried adding some azure II blue
} dye I had on hand to the agar so I could see the agar blocks more
} easily, but by the time I got up to epon/acetone, the color was gone.
} Any suggestions for a dye that will withstand both the water based and
} acetone solutions, or an alternative way to mark the little agar blocks
} so I can more easily monitor them throughout the process?
}
} Thanks in advance
}
} Steve
}
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--
__ ___ ^ ___ ___ Tobias I. Baskin
/ \ / / \ / \ Professor
/ / / / \ \ \ Biology Department
/ __/ /__ /___ \ \ \__ University of Mass.
/ / / \ \ \ 611 N. Pleasant St.
/ / / \ \ \ Amherst, Massachusetts
/ /___ / \ \___/ \_____ USA 413-545-1533
www.bio.umass.edu/biology/baskin


==============================Original Headers==============================
4, 23 -- From baskin-at-bio.umass.edu Mon Feb 1 16:40:07 2016
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4, 23 -- From: Tobias Baskin {baskin-at-bio.umass.edu}
4, 23 -- Subject: Re: [Microscopy] viaWWW:TEM: agar embedding cells
4, 23 -- To: Microscopy-at-microscopy.com, sbarlow-at-mail.sdsu.edu
4, 23 -- References: {201601300144.u0U1irGV011027-at-ns.microscopy.com}
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From: nizets2-at-yahoo.com
Date: Tue, 2 Feb 2016 05:00:37 -0600
Subject: [Microscopy] viaWWW:TEM: agar embedding cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Steve!

Not directly answering your question but making a remark:

You are working with a cell monolayer, meaning approx. 50-um thin layer.
This is so thin that you don't have to care about fluid exchange, I don't think that cutting your agar in small blocks will add anything.
I would keep the whole agar block standing at the bottom of a jar, so you always know that the cells are on the upper side.

Regards
Stephane



--------------------------------------------
On Mon, 2/1/16, baskin-at-bio.umass.edu {baskin-at-bio.umass.edu} wrote:

Subject: [Microscopy] Re: viaWWW:TEM: agar embedding cells
To: nizets2-at-yahoo.com
Date: Monday, February 1, 2016, 11:48 PM




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Hi,
-* -*-*-*I have had luck with fast green
added at the 100% ethanol step. I
make a saturated fast green solution in ethanol and add a
drop or two at
the 100% ethanol step. It kept the agar blocks rather
colored through
subsquent infiltrations and polymerization. Sorry I don't
have handy
what "saturated" means in terms of mg/mL but if you are
interested I
could probably dig that up somewhere.
Good luck,
-* -* -* -* -* -* -* -*
-* -* -* -*-*-*Tobias

On 1/29/16 8:44 PM, microscopy.listserver-at-gmail.com
wrote:
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} Email: sbarlow-at-mail.sdsu.edu
} Name: Steve Barlow
}
} Organization: SDSU EM Facility
}
} Title-Subject: [Filtered] TEM: agar embedding cells
}
} Message: Some advice please on colorizing agar for
embedding cells.-* In
} the past, my osmicated pellets in agar blocks were
easily trackable, but
} when the cells are in a-* thin layer the cells
don't give much contrast,
} especially if not osmicated. I am collecting naked
eukaryotic cells in
} suspension culture, but have problems bring down the
smaller cells by
} centrifugation.-* Cultures containing cells are
fixed in aldehyde or
} aldehyde/osmium cocktail, collected onto millipore
Nucleopore filters,
} then held in place by adding warm agar over them on the
filter.
}
} When cooled, I peel off the filter, leaving me with a
thin layer of
} cells embedded in the agar.-* I then cut the agar
layer into small blocks
} to enhance fluid exchange during subsequent treatments.
My problem is,
} the cells are light colored to faintly black, and the
thin layer of
} cells does not stand out well, particularly when in the
various stages
} of epon infiltration/polymerization.-* I tried
adding some azure II blue
} dye I had on hand to the agar so I could see the agar
blocks more
} easily, but by the time I got up to epon/acetone, the
color was gone.
} Any suggestions for a dye that will withstand both the
water based and
} acetone solutions, or an alternative way to mark the
little agar blocks
} so I can more easily monitor them throughout the
process?
}
} Thanks in advance
}
} Steve
}
} -* -* Login Host: 146.244.234.237
} -* -* Listserver Email Form V - 20120416
}
---------------------------------------------------------------------------
}
}
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}

--
-* -* -*-*-*__-* -*
___-*-*-*^-* -*
___-*-*-*___-*-*-*Tobias I. Baskin
-* -* -* /-* \-* /-*
-*-*-*/ \-* /-* -* -* \-*
-* -* Professor
-* -*-*-*/-*-*-*/ /-*
-*-*-*/-*-*-*\ \-* -*
-*-*-*\-* -* -* Biology Department
-* -* / __/ /__-*-*-*/___-* \
\-* -* -*-*-*\__-* -* University
of Mass.
-*-*-*/-* -*-*-*/-*
-*-*-*/-* -* -*-*-*\ \-*
-* -*-*-*\-* -* -* 611 N.
Pleasant St.
-* /-* -*-*-*/-*
-*-*-*/-* -* -* -*-*-*\
\-* -* -*-*-*\-* -* -*
Amherst, Massachusetts
/-* -*-*-*/___-* /-* -* -*
-* -*-*-*\ \___/-*-*-*\_____
USA-* 413-545-1533
-*-*-*www.bio.umass.edu/biology/baskin


==============================Original
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4, 23 -- Subject: Re: [Microscopy] viaWWW:TEM: agar
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From: CGorman-at-hookecollege.com
Date: Tue, 2 Feb 2016 07:09:31 -0600
Subject: [Microscopy] Short Course Announcement: TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings Microscopists,

The Hooke College of Applied Sciences, located in Westmont, IL, is offering its transmission electron microscopy short course April 5-7, 2016.
In addition to lectures, this course emphasizes hands-on training using state-of-the-art equipment. For further TEM training details and registration information, please follow the link below:

https://www.mccrone.com/transmission-electron-microscopy-tem-course


Best regards-
__________________________________________________
Chris Gorman
Admissions Specialist
Hooke College of Applied Sciences
850 Pasquinelli Drive
Westmont, IL 60559-5539 USA
630-887-7100 (tel)
www.hookecollege.com


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From: microscopy.listserver-at-gmail.com
Date: Tue, 2 Feb 2016 14:25:19 -0600
Subject: [Microscopy] viaWWW:Job opening: Research Associate in Nuclear Graphite

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X-from: h.wu2-at-lboro.ac.uk
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Email: h.wu2-at-lboro.ac.uk
Name: Houzheng Wu

Organization: Loughborough University UK

Title-Subject: [Filtered] Job opening: Research Associate in Nuclear
Graphite

Message: Research Associate in Nuclear Graphite
http://www.jobs.ac.uk/job/AMX298/research-associate-in-nuclear-graphite/

Loughborough University - Department of Materials
Location: Loughborough, UK
Salary: #28,982 to #31,656 per annum
Hours: Full Time
Contract Type: Contract / Temporary
Placed on: 2nd February 2016
Closes: 7th February 2016
Job Ref: REQ15383

Fixed term for 33 months Understanding and Improving Graphite for
Advanced Nuclear Fission (UNIGRAF) Required to undertake experimental
research on structure from atomic- to micro-scale of iso-graphite for
advanced nuclear reactors. The project aims to improve the capability of
nuclear graphite through a better understanding of irradiation-induced
changes in structure and their impact on mechanical/physical behavior.
The post is part of an EPSRC funded project "UNIGRAF" in partnership
with Oxford and Bristol University, and multi supporters in USA, China
and Germany. Based at Loughborough the post holder will have strong
collaborations with scientists/engineers from all partners, and focus on
structure characterisation before and after irradiation using HRTEM,
EELS, FIB and other techniques. You will have opportunity to access
structural characterisation facilities in Oak Ridge National Laboratory,
Oxford, and EPSRC National Facility, and multi overseas travel is expected.

A good honours degree (2.1 minimum) and a PhD or equivalent in Materials
Science, Physics, or another relevant discipline and experience in
materials structural characterisation techniques is essential. Research
in graphite/graphene materials and knowledge in nuclear materials is
desirable. Application closing date: 7 February 2016


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From: microscopy.listserver-at-gmail.com
Date: Tue, 2 Feb 2016 14:26:16 -0600
Subject: [Microscopy] viaWWW:Electron Microscopy Position at Naval Postgraduate School,

Contents Retrieved from Microscopy Listserver Archives
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Email: skmeno1-at-nps.edu
Name: Sarath K Menon

Organization: NAVAL POSTGRADUATE SCHOOL

Title-Subject: [Filtered] Electron Microscopy Position at Naval
Postgraduate School, Monterey, CA

Message: Research Assistant Professor,
AD-1701-03
Department of Mechanical and Aerospace Engineering
Naval Postgraduate School, Monterey, CA

The Mechanical and Aerospace Engineering Department of the Naval
Postgraduate School is inviting applications from electron Microscopy
Specialists to fulfill a Research Faculty position to support the
research and development of a variety of materials including metals,
ceramics, carbon-based materials and composites. The position will be
focused on the operation of several advanced characterization
techniques, including fully equipped Scanning Electron Microscope and
Transmission Electron Microscope. Responsibilities of the position
include the maintenance and upkeep of these complex analytical
instruments. The applicant should possess operational expertise with
SEM, FIB, TEM lamellae preparation, TEM operation including STEM
methods, high resolution TEM and analytical techniques such as EDS,
EBSD, EELS and EFTEM. The candidate for the position must be able to
perform sample analysis with minimal direction and/or oversight from
technical principal investigators. The candidate must also be able to
collect, assimilate, and interpret data for technical principal
investigators and write technical reports to document results. The
position description includes also the coordination of all services
required to keep instruments operational, the procurement of all
consumables for the microscopy lab and to carry out all the routine
maintenance tasks. A PhD in Materials Science or a closely related field
is required. A few years of hands-on experience with current
state-of-the-art instrumentation and techniques in the area of electron
microscopy (SEM, TEM, STEM, EFTEM and FIB) is mandatory. The ability to
teach a course related to Advanced Materials Characterization techniques
will be highly valued. In addition, the candidate is expected to train
Masters level students to use instruments and help them with data
analysis. The ability to obtain a US security clearance is mandatory.

Applications should be received by 1 April 2015 and include a curriculum
vitae, writing sample, summary of available teaching evaluations, and
three letters of recommendation. Please address the applications to:

Garth V. Hobson
Professor and Chair
Department of Mechanical & Aerospace Engineering
700 Dyer Road, Bldg 245 -n Watkins Hall, WA-338
Naval Postgraduate School
Monterey, CA 93943
TEL: 831-656-2586/831-656-2888
www.nps.edu/mae
gvhobson-at-nps.edu



Salary is commensurate with qualifications and experience. Relocation
package, including recruitment/relocation incentive may be authorized.
The position will remain open until filled.
The Naval Postgraduate School is an equal opportunity employer. For
additional information about NPS, please refer to the website at
http://www.nps.edu.


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21, 52 -- Subject: viaWWW:Electron Microscopy Position at Naval Postgraduate School,
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From: microscopy.listserver-at-gmail.com
Date: Tue, 2 Feb 2016 16:10:15 -0600
Subject: [Microscopy] viaWWW:Calling all Graduates of Madison Area Technical College

Contents Retrieved from Microscopy Listserver Archives
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X-from: AThiessen-at-wisc.edu

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Email: AThiessen-at-wisc.edu
Name: Anna Thiessen

Organization: Electron Microscopy Program/ Madison Area Technical College

Title-Subject: [Filtered] Calling all Graduates

Message: Hey Listservers we need your help!
The Electron Microscopy Program at Madison Area Technical College in
Madison, Wisconsin is closing its door after 24 years. The present and
past program directors (Michael Kostrna and Glenn Boda) are trying to
organize a reunion/-iwake-i for the program at the end of May.
Unfortunately, after 24 years a lot of contact information has been
changed and not updated! If you or anyone you know graduated or
participated in the program please pass the word on. If you would like
to find out more information, please contact Jennifer Kostrna at
kostrna.emwakeparty-at-gmail.com.

Many Thanks,
Anna Thiessen (Recent Graduate)

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From: microscopy.listserver-at-gmail.com
Date: Wed, 3 Feb 2016 05:00:28 -0600
Subject: [Microscopy] viaWWW:Free Kevex Sigma EDX System for SEM

Contents Retrieved from Microscopy Listserver Archives
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Email: Hobie-at-technicalsalessolutions.com
Name: Hobie Richards

Organization: Technical Sales Solutions

Title-Subject: [Filtered] Free Kevex Sigma EDX System for SEM

Message: TSS has one complete Kevex Sigma EDX system, that needs a good
home. Detector (window looks good), collimator, Sigma Analyzer, 4855
Digital Beam Control Interface Module, and cable set. Was installed on
XL 30 W SEM.

Pictures may be seen here:
https://tss.exavault.com/share/view/a4yp-7l4eat7f

Will put in a box and with your shipping label send to you anywhere in
the world.

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From: microscopy.listserver-at-gmail.com
Date: Thu, 4 Feb 2016 13:37:18 -0600
Subject: [Microscopy] viaWWW:Speckled TEM Samples

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Name: Debra M Townley

Organization: Baylor College of Medicine

Title-Subject: [Filtered] Speckled TEM Samples

Message: This is awful - I am having a terrible time getting clean TEM
sections. I filter the primary fix mixture with a 0.1um vacuum
filtration unit; I've tried changing the water and am currently using
RICCA ultra-pure H2O; I have stopped en bloc staining altogether; I use
Spurr's Low Viscosity embedding resin. Most recently I have been
ethanol-washing all razor blades used during specimen collection; I use
a 0.04% FSG quench after fixation (didn't seem to make a difference).
The fine speckling is most noticeable in muscle tissue, although I see
it in other tissues as well. I just looked at some freshly cut,
unstained mouse foot pad muscle yesterday and there were the black
speckles all over the place! The specks are tiny, much smaller than
10nm. It almost looks like immuno-gold, except that I haven't done
immuno-gold staining in years, and the specks are not rounded as you
might expect with gold particles. They don't seem to be clustered at any
specific organelle, and they are definitely in the tissue, not on the
surface. Someone please help, this is driving me nuts!

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From: mdelann1-at-jhmi.edu
Date: Thu, 4 Feb 2016 14:08:56 -0600
Subject: [Microscopy] viaWWW:Speckled TEM Samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Debra,

This could be a couple of things. First did you use phosphate buffer? If
your concentration was a 100 mM or over, the Ga, osmium and PO4 can
precipitate in the tissue. Check unstained sections to confirm.
Also make sure all the Ga is rinsed out of tissue before you get to osmium.
Are the tissue pieces small? You can also microwave fixative to get better
penetration. Be careful with using reduced osmium and then staining muscle.
Lipid granules are affected with the post-section staining with UA and Pb.
Apparently the lipid material gets lost after staining and rinsing, I have
seen this phenomena and sometimes it will contaminate parts of the section.
If you are sure it isn't your staining protocol, then it must be the
fixation.

Good Luck,
Michael Delannoy

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Email: debrat-at-bcm.edu
Name: Debra M Townley

Organization: Baylor College of Medicine

Title-Subject: [Filtered] Speckled TEM Samples

Message: This is awful - I am having a terrible time getting clean TEM
sections. I filter the primary fix mixture with a 0.1um vacuum filtration
unit; I've tried changing the water and am currently using RICCA ultra-pure
H2O; I have stopped en bloc staining altogether; I use Spurr's Low Viscosity
embedding resin. Most recently I have been ethanol-washing all razor blades
used during specimen collection; I use a 0.04% FSG quench after fixation
(didn't seem to make a difference).
The fine speckling is most noticeable in muscle tissue, although I see it in
other tissues as well. I just looked at some freshly cut, unstained mouse
foot pad muscle yesterday and there were the black speckles all over the
place! The specks are tiny, much smaller than 10nm. It almost looks like
immuno-gold, except that I haven't done immuno-gold staining in years, and
the specks are not rounded as you might expect with gold particles. They
don't seem to be clustered at any specific organelle, and they are
definitely in the tissue, not on the surface. Someone please help, this is
driving me nuts!

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21, 23 -- From prvs=835ad12fc=mdelann1-at-jhmi.edu Thu Feb 4 14:08:56 2016
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From: mdelann1-at-jhmi.edu
Date: Thu, 4 Feb 2016 14:37:08 -0600
Subject: [Microscopy] viaWWW:Speckled TEM Samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Debra,

This could be a couple of things. First did you use phosphate buffer? If
your concentration was a 100 mM or over, the Ga, osmium and PO4 can
precipitate in the tissue. Check unstained sections to confirm.
Also make sure all the Ga is rinsed out of tissue before you get to osmium.
Are the tissue pieces small? You can also microwave fixative to get better
penetration. Be careful with using reduced osmium and then staining muscle.
Lipid granules are affected by the post-section staining with UA and Pb.
Apparently the lipid material gets lost after staining and rinsing, I have
seen this phenomena and sometimes it will contaminate parts of the section.
If you are sure it isn't your staining protocol, then it must be the
fixation.

Good Luck,
Michael Delannoy


-----Original Message-----
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To: mdelann1-at-jhmi.edu

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Email: debrat-at-bcm.edu
Name: Debra M Townley

Organization: Baylor College of Medicine

Title-Subject: [Filtered] Speckled TEM Samples

Message: This is awful - I am having a terrible time getting clean TEM
sections. I filter the primary fix mixture with a 0.1um vacuum filtration
unit; I've tried changing the water and am currently using RICCA ultra-pure
H2O; I have stopped en bloc staining altogether; I use Spurr's Low Viscosity
embedding resin. Most recently I have been ethanol-washing all razor blades
used during specimen collection; I use a 0.04% FSG quench after fixation
(didn't seem to make a difference).
The fine speckling is most noticeable in muscle tissue, although I see it in
other tissues as well. I just looked at some freshly cut, unstained mouse
foot pad muscle yesterday and there were the black speckles all over the
place! The specks are tiny, much smaller than 10nm. It almost looks like
immuno-gold, except that I haven't done immuno-gold staining in years, and
the specks are not rounded as you might expect with gold particles. They
don't seem to be clustered at any specific organelle, and they are
definitely in the tissue, not on the surface. Someone please help, this is
driving me nuts!

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Listserver Email Form V - 20120416
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From: wij.muss-at-aon.at
Date: Fri, 5 Feb 2016 04:40:28 -0600
Subject: [Microscopy] Re: Speckled TEM Samples

Contents Retrieved from Microscopy Listserver Archives
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Dear Debra,

thank you for your thorough listing of your processing step...
what you omit, what you changed, etc., etc...
Nevertheless you ought to tell us about the
== buffer vehicle in fixation as well as after fixation
== osmication data
== processing schedule right after osmium into ascending alcohols,
the latter most important to know which concentration you start.
Could it be that you face "salt & pepper" artifact?
It would be interesting to see an image of your speckles...

Best regards,
Wolfgang

Wolfgang MUSS, PhD
Member of MSA until 2015
Retired by Dec. 1st, 2015
SALZBURG, AUSTRIA
wij.muss-at-aon.at



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This is awful - I am having a terrible time getting clean TEM sections.
I filter the primary fix mixture with a 0.1um vacuum filtration unit;
I've tried changing the water and am currently using RICCA ultra-pure H2O;
I have stopped en bloc staining altogether;
I use Spurr's Low Viscosity embedding resin.
Most recently I have been ethanol-washing all razor blades used during
specimen collection;
I use a 0.04% FSG quench after fixation (didn't seem to make a difference).
The fine speckling is most noticeable in muscle tissue, although I see it in
other tissues as well. I just looked at some freshly cut, unstained mouse
foot pad muscle yesterday and there were the black speckles all over the
place! The specks are tiny, much smaller than 10nm. It almost looks like
immuno-gold, except that I haven't done immuno-gold staining in years, and
the specks are not rounded as you might expect with gold particles.
They don't seem to be clustered at any specific organelle, and they are
definitely in the tissue, not on the surface. Someone please help, this is
driving me nuts!

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From: microscopy.listserver-at-gmail.com
Date: Fri, 5 Feb 2016 06:04:14 -0600
Subject: [Microscopy] viaWWW:Image of speckled mouse muscle.

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Name: bandic00t

Organization: Baylor College of Medicine

Title-Subject: [Filtered] Speckled TEM sections

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Image of speckled mouse muscle.

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From: benada-at-biomed.cas.cz
Date: Fri, 5 Feb 2016 07:23:55 -0600
Subject: [Microscopy] RE: viaWWW:Image of speckled mouse muscle.

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Hello Debra,
Thank you for showing us the image with "speckles". I think that the problem might be in lead staining procedure. Please, look at Arvid B. Maunsbach & Bj/drn A. Afzelius "Biomedical Electron Microscopy (Illustrated Methods and Interpretations)" 1999, Pages 207,Aei228 {doi:10.1016/B978-012480610-8/50011-1} for explanation. We had also similar problems with lead carbonate precipitations in the past. Since our technician started to wear a face shield during sections staining with lead citrate the precipitates (contamination) in them almost vanished.

There is a old paper on "How to remove such precipitates from ultrathin sections":
Kuo, J. (1980), A simple method for removing stain precipitates from biological sections for transmission electron microscopy. Journal of Microscopy, 120: 221,Aei224. {doi:10.1111/j.1365-2818.1980.tb04140.x}

We tried this approach several times, but the results were not ideal in our hands.


Best regards

Oldrich

--
Oldrich Benada
Institute of Microbiology CAS, v.v.i.
Videnska 1083
142 20 Prague 4
Czech Republic

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From: nsa2-at-leicester.ac.uk
Date: Fri, 5 Feb 2016 10:37:09 -0600
Subject: [Microscopy] viaWWW:Speckled TEM Samples

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Hi Debra,

Difficult to say when we don't know your primary buffer / fixation protocol, but I would agree that it looks like it may be due to either precipitate from a phosphate buffer, possibly due to insufficient washing between Ga/OSO4 steps, or osmium 'peppering', usually produced with buffer reactions in the osmium steps, or prolonged fixation in osmium.

I used to have similar problems, and resolved them by changing my buffer to HEPES or cacodylate, extra wash steps between the glutaraldehyde and osmium fixes, and secondary fixing in aqueous osmium as opposed to buffered osmium.

Good luck resolving it - let us know how you get on!

Natalie


Miss Natalie Allcock
EM Facility Manager

Electron Microscopy Facility,
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University of Leicester, University Road, Leicester, LE1 7RH, UK
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Email: debrat-at-bcm.edu
Name: Debra M Townley

Organization: Baylor College of Medicine

Title-Subject: [Filtered] Speckled TEM Samples

Message: This is awful - I am having a terrible time getting clean TEM sections. I filter the primary fix mixture with a 0.1um vacuum filtration unit; I've tried changing the water and am currently using RICCA ultra-pure H2O; I have stopped en bloc staining altogether; I use Spurr's Low Viscosity embedding resin. Most recently I have been ethanol-washing all razor blades used during specimen collection; I use a 0.04% FSG quench after fixation (didn't seem to make a difference).
The fine speckling is most noticeable in muscle tissue, although I see it in other tissues as well. I just looked at some freshly cut, unstained mouse foot pad muscle yesterday and there were the black speckles all over the place! The specks are tiny, much smaller than 10nm. It almost looks like immuno-gold, except that I haven't done immuno-gold staining in years, and the specks are not rounded as you might expect with gold particles. They don't seem to be clustered at any specific organelle, and they are definitely in the tissue, not on the surface. Someone please help, this is driving me nuts!

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From: microscopy.listserver-at-gmail.com
Date: Fri, 5 Feb 2016 13:56:32 -0600
Subject: [Microscopy] viaWWW:Leitz Aristoplan microscope detailled service manual

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Email: jmhermel-at-inaf.cnrs-gif.fr
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Title-Subject: [Filtered] Leitz Aristoplan microscope detailled service
manual

Message: Hi,
I should appreciate any help or link to find a Leitz Aristoplan
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From: microscopy.listserver-at-gmail.com
Date: Fri, 5 Feb 2016 13:58:42 -0600
Subject: [Microscopy] viaWWW:Open EM Position at the Molecular Foundry

Contents Retrieved from Microscopy Listserver Archives
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Email: mlibbee-at-lbl.gov
Name: Marissa Libbee

Organization: LBL

Title-Subject: [Filtered] Open Position at the Molecular Foundry

Message: See description below. For more information, please follow the
link http://jobs.lbl.gov/open-positions.html


Molecular Foundry Research Scientist-81646
Organization:MS-Materials Sciences
Description

Berkeley Lab is Bringing Science Solutions to the World, and YOU can be
a part of it!

In the world of science, Lawrence Berkeley National Laboratory (Berkeley
Lab) is synonymous with "excellence." That's why we hire the best -
whether in research, finance or other operations. This is a great
opportunity to bring your top-notch skills to bear in support of
world-class scientific research that addresses national and global
challenges!

Position Summary:
The National Center for Electron Microscopy (NCEM) facility in the
Molecular Foundry at Lawrence Berkeley National Laboratory is seeking a
Research Scientist to establish an internationally recognized theory and
image simulation research program to advance the state of the art in
electron microscopy. The primary responsibilities are: 1) to develop
theory and methodology for quantitative comparison between theoretical
models and data from advanced electron microscopy instruments, and 2) to
lead the development of new techniques and instrumentation for electron
scattering. This position will require working on a wide range of
collaborative research projects in materials characterization and
establishment of an outstanding user program and individual research
program.

What You Will Do:
Develop image processing and simulation tools to enhance capabilities
for quantitative data analysis.
Develop an independent research program. Lead or participate in the
development of new research directions.
Offer computational support for ultrahigh resolution microscopy in
aberration-corrected instruments in TEM and STEM. Devise new approaches
to overcoming limitations of exit wave reconstruction routines and
implement these approaches in NCEM-is user program. Refine theory and
simulation for STEM imaging to quantify image analysis.
Contribute creatively to the development of techniques for atomic
resolution tomography.
Establish protocols and work flows for the effective management of large
data sets for in-house and remote analysis
Participate in the design and implementation of new techniques for
imaging of energy-related materials, including enhanced visibility and
analysis of light elements, imaging at low dose, analysis of defects,
and imaging of thick samples.
Provide theoretical insight and support as part of a team to develop new
detectors, holography, and other instrumentation and techniques.
Lead an effort to help close the gap between experimentally available
data and theoretically accessible systems.
Be familiar with materials modeling and computational procedures for
direct comparison with experimental observations. Modeling approaches
could range from macroscopic to atomistic methods, from elastic or
thermodynamic to kinetic or first principles techniques.
Serve as point of contact for the theory and simulation requirements of
users related to nanoscale phenomena accessible to electron microscopy
imaging. Oversee operation of image analysis and computer lab at NCEM.
Provide expertise in the application of existing commercial software and
the development of custom modules and scripts.
Serve as scientific contact for user proposals. Critically evaluate
scientific proposals for feasibility; design research plans and
appropriate microscopy experiments to accomplish specific research
objectives. Improve quality of the analysis component of user projects
by providing theory and simulation support and collaboration.
Work closely with other Molecular Foundry staff scientists.
Disseminate research results through publications and presentations at
national and international conferences.
What Is Required:
Proven ability to develop and apply computational methods for image
analysis, image simulation and advanced codes for modeling of materials
behavior.
Excellent scientific publications record in relevant areas
Well-developed oral and written communication skills. Ability to work
effectively within a team and with various levels of internal and
external users and staff.
Experience in the management and manipulation of large data sets
commensurate with high resolution electron microscopy
Ph.D. or equivalent experience in the Physical Sciences or Engineering
Strong organizational and time management skills. Ability to manage
competing priorities, provide quality work within tight time
constraints, and deliver projects on schedule.
Additional Desired Qualifications:
Experience with computational materials modeling such as density
functional theory, molecular dynamics, etc.
Strong background of work with experimental data and analysis of images
from in situ experiments.

Notes: This is a career appointment.

PLEASE NOTE: This job will close Feb 7, at midnight.

This position requires completion of a background check.

Berkeley Lab addresses the world-is most urgent scientific challenges by
advancing sustainable energy, protecting human health, creating new
materials, and revealing the origin and fate of the universe. Founded in
1931, Berkeley Lab-is scientific expertise has been recognized with 13
Nobel prizes. The University of California manages Berkeley Lab for the
U.S. Department of Energy-is Office of Science.

Equal Employment Opportunity: Berkeley Lab is an Equal
Opportunity/Affirmative Action Employer. All qualified applicants will
receive consideration for employment without regard to race, color,
religion, sex, sexual orientation, gender identity, national origin,
disability, age, or protected veteran status. Berkeley Lab is in
compliance with the Pay Transparency Nondiscrimination Provision under
41 CFR 60-1.4. Click here to view the poster and supplement: "Equal
Employment Opportunity is the Law."

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From: microscopy.listserver-at-gmail.com
Date: Fri, 5 Feb 2016 13:59:44 -0600
Subject: [Microscopy] viaWWW:Research and Development Scientist UK Job Opening

Contents Retrieved from Microscopy Listserver Archives
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Email: jhyun-at-gatan.com
Name: John Hyun

Organization: Gatan, Inc.

Title-Subject: [Filtered] Research and Development Scientist UK Job Opening

Message: Location: Abingdon, Oxfordshire UK

Gatan is an industry leader in the electron microscopy industry and the
Research & Development Scientist will work as part of multidisciplinary
teams of scientists and development engineers. The role will provide
technical input and deliver innovative technology to new product
introductions, meeting project schedules and budgets. There will be a
requirement for collaboration across all business functions working
closely with the Marketing, Manufacturing and Customer Service teams to
assure the success of all new product introductions. The building of
relationships and working closely with internal and external customers
will be a key requirement.

The prime responsibility will be to engage in product development tasks
according to Gatan-is product life cycle (PLC) model, a phased process
that includes concept and feasibility, design and development, alpha and
beta pilot phases leading to product release. Other responsibilities
will involve sustaining engineering for mature and legacy products along
with the provision of engineering support to Gatan-is production and
customer service teams. The product range and technical challenges are
diverse and so the ideal candidate will have a broad range of scientific
and engineering skills.

The successful candidate will be cable of working semi-autonomously on
multiple tasks and must be capable of providing innovative solutions to
technical challenges in high technology product design and development.
Equally important will be a keen eye for cost effective solutions that
are designed for test, manufacture and service.
The role will report directly to the Director of Engineering (UK) and
some UK and international travel will be required.

For a full description and application instructions, visit:
http://www.gatan.com/company/careers/research-and-development-scientist



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From: microscopy.listserver-at-gmail.com
Date: Mon, 8 Feb 2016 14:38:17 -0600
Subject: [Microscopy] viaWWW:Oxford INCA documentation

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Email: opmills-at-mtu.edu
Name: Owen Mills

Organization: Michigan Tech University

Title-Subject: [Filtered] Oxford INCA documentation

Message: Hello

We hope someone on the list has a copy (paper or electronic) of the
Oxford INCA User Manual. We were given an 80 page INCA Operator Manual
document that provides only a cursory explanation of topics like
managing the master standards database. For comparison the Aztec manual
is 500 pages. Maybe what we are seeking doesn't exist, but if you do
have a manual we'd be happy to pay copying or shipping costs.

Any help you can supply would be appreciated.

Cheers
Owen
--
Owen P Mills
Director, Applied Chemical and Morphological Analysis Laboratory
Michigan Technological University
1400 Townsend Dr
Houghton, MI 49931
906-369-1875
opmills-at-mtu.edu
http://mcff.mtu.edu/acmal/

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From: microscopy.listserver-at-gmail.com
Date: Mon, 8 Feb 2016 14:39:06 -0600
Subject: [Microscopy] viaWWW:TEM - Cryo-ultramicrotomy of rubber sections

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Email: badamianand-at-bfusa.com
Name: Anand Badami

Organization: Bridgestone Research

Title-Subject: [Filtered] TEM - Cryo-ultramicrotomy of rubber sections

Message: Can anyone recommend a technique for collecting rubber sections
during cryo-ultramicrotomy that enables the sections to lie flat (i.e.
as wrinkle free as possible) on a TEM support grid? I currently collect
my sections off the back of my cryoknife using a sucrose droplet, but as
the sections warm coming out of the cryochamber on the droplet they
contract and wrinkle before being deposited on a TEM grid (with or
without carbon coating). Chamber temperature is -120 C. Any ideas are
appreciated. Thank you.

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From: microscopy.listserver-at-gmail.com
Date: Mon, 8 Feb 2016 14:45:08 -0600
Subject: [Microscopy] viaWWW:Upcoming AFM/SPM courses in US, Europe

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Email: dalia.yablon-at-surfacechar.com
Name: Dalia Yablon

Organization: SurfaceChar

Title-Subject: [Filtered] Upcoming AFM/SPM courses in US, Europe

Message: Dear colleagues,

There are several scanning probe microscopy/atomic force microscopy
courses that are happening this spring in both the US and Europe:

April 5-7, 2016 Netherlands: Overview of Scanning Probe Microscopy: A
hands-on short course covering the technology, basics of operation, and
applications. This course has a March 5, 2016 registration deadline.
For course details and registration, go to
http://www.surfacechar.com/classesschedule.html

May 10-11, 2016 Boston MA: AFM for Characterization of Polymer
Materials. A 2-day hands on course focusing on AFM for polymer
applications to study polymer structure, morphology, and materials
properties. For course details and registration, go to
http://www.afmworkshop.com/characterization-of-polymer-materials-afm-training.html

May 22, 2016 Washington DC: Nanoscale Materials Characterization for
the Biomedical and Pharmaceutical Industry. For course details and
registration, go to http://techconnectworld.com/World2016/workshops/518.html

Sincerely,

Dalia Yablon, Ph.D.
SurfaceChar LLC
www.surfacechar.com
Dalia.yablon-at-surfacechar.com


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From: leunissen-at-aurion.nl
Date: Mon, 8 Feb 2016 16:06:47 -0600
Subject: [Microscopy] Re: viaWWW:TEM - Cryo-ultramicrotomy of rubber sections

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Good morning Anand,

if the wrinkling happens during warming of the droplet, could it be because of the section surface characteristics?
Adding salt to the sucrose should help if the surface is charged, multivalent ion salts would be more suited than monovalent.
However if the surface is not charged but hydrophobic adding salt would probably increase the wrinkling, and in that case adding a hydrophobic component might help, such as serum albumin. Some detergents can do both.

Good luck!


Jan Leunissen
Dunedin, NZ

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} Email: badamianand-at-bfusa.com
} Name: Anand Badami
}
} Organization: Bridgestone Research
}
} Title-Subject: [Filtered] TEM - Cryo-ultramicrotomy of rubber sections
}
} Message: Can anyone recommend a technique for collecting rubber sections
} during cryo-ultramicrotomy that enables the sections to lie flat (i.e.
} as wrinkle free as possible) on a TEM support grid? I currently collect
} my sections off the back of my cryoknife using a sucrose droplet, but as
} the sections warm coming out of the cryochamber on the droplet they
} contract and wrinkle before being deposited on a TEM grid (with or
} without carbon coating). Chamber temperature is -120 C. Any ideas are
} appreciated. Thank you.
}
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From: microscopy.listserver-at-gmail.com
Date: Tue, 9 Feb 2016 15:27:21 -0600
Subject: [Microscopy] viaWWW:ipj oxford eds file

Contents Retrieved from Microscopy Listserver Archives
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Title-Subject: [Filtered] ipj oxford eds file

Message: Does any body know a tool/library for opening the Oxford Inca
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From: smithj-at-winthrop.edu
Date: Wed, 10 Feb 2016 07:28:06 -0600
Subject: [Microscopy] Need contact information for Leica Microscopy rep, South Carolina,

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Sorry to spam your in-boxes folks.

My response to Harry.Clark-at-leica-microsystems.com has bounced twice with
"content rejected"

Need a quote on a basic upright biological microscope with
transmitted-light DIC (DM2500).

If you're the person mentioned in the subject line, please respond to my
address below.

Thanks,
Julian

--
Julian P.S. Smith III
Director, Winthrop Microscopy Facility
Dept. of Biology
Winthrop University
349 Columbia Ave
Rock Hill, SC 29733

803-323-2111 x6427 (vox)
803-323-3448 (fax)
803-524-2347 (cell)
Research Website www.birdnest.org/smithj
Personal Website www.rociada-east.net


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From: ALawrence-at-i2at.msstate.edu
Date: Wed, 10 Feb 2016 14:04:40 -0600
Subject: [Microscopy] M&M 2016 student and volunteer meeting assistance

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The hotel reservation link is open and the extended deadline for paper submission is less than a week away. As you (or your students) are making plans to attend the Columbus meetings (July 24-28), please don't forget about MSA's student bursary program. Its purpose is to encourage students to attend the meetings by helping to defray some of the costs while giving them an opportunity to meet and interact with the established microscopy community.

The students will be paid $10 an hour to work for ~20 hours during the meeting or pre-meeting events. The jobs involve such things as providing support in the different symposia (assisting with audio-visual needs, speaker set-up, maintaining an attendance count), staffing the volunteer office, and helping with vendor tutorial sign-up. Payment is given as a check at the end of the meetings or when the student leaves Columbus.

Once the program has been finalized, each registered bursary will be contacted and allowed to choose the times and activities they would like to work. Many times they end up "working" sessions they would attend anyway. There is an added bonus of $10 cash to help with meals for each morning and/or afternoon session worked and a meeting shirt.

If anyone would like to participate in the bursary program be sure to check the "I wish to apply for a student bursary" box in section 2 of the registration form (registration opens March 1) and send me an email of your intention. Bursary space is limited, so sign-up early. Applicants for the bursaries must be members of MSA or MAS, enrolled as students at a recognized educational institution, and have paid their registration fee.

For those 'non-students' volunteers are also needed to help with the above mentioned meeting activities. Although not paid on an hourly basis as the student bursaries, volunteers do receive a meeting shirt and the same cash allotment to help with meals. Volunteers also have the opportunity to interact more with the microscopy community as they assist with meeting tasks.

If anyone has any questions about the bursary/volunteer program, or would like to participate, please contact me.
Amanda

Amanda Lawrence
Institute for Imaging and Analytical Technologies
Mississippi State University
662-325-7998
alawrence-at-i2at.msstate.edu




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From: microscopy.listserver-at-gmail.com
Date: Wed, 10 Feb 2016 22:40:09 -0600
Subject: [Microscopy] viaWWW:Stanford Imaging Workshop: Advanced applications of high speed

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Email: alkoh-at-stanford.edu
Name: Ai Leen Koh

Organization: Stanford University

Title-Subject: [Filtered] Stanford Imaging Workshop: Advanced
applications of high speed imaging in S/TEM

Message: Friday, March 11, 2016
8:30 am - 5:00 pm
Stanford University
Stanford Nano Shared Facilities
94305-4088 Stanford , CA

Stanford Nano Shared Facilities (SNSF) invites you to attend this unique
S/TEM workshop demonstrating the latest high speed imaging technology
and applications specifically for in-situ TEM and 4D STEM diffraction.

This comprehensive, full-day workshop will feature lectures by leading
experts, open Q&A sessions, and live microscope demonstrations all in
the state-of-the-art SNSF research complex. The microscope sessions will
be performed on an aberration (image) corrected, monochromated FEI Titan
environmental (S)TEM with a OneView in-situ camera.

This workshop is complimentary to all registered and confirmed
participants and includes breakfast, lunch and refreshments. Seating is
limited.

Online registration:
http://www.gatan.com/company/events/stanford-imaging-workshop-advanced-applications-high-speed-imaging-stem


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From: microscopy.listserver-at-gmail.com
Date: Wed, 10 Feb 2016 22:43:01 -0600
Subject: [Microscopy] viaWWW:Microscopy Sales Opportunity- Bay Area

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Name: Mary Southgate Dickson

Organization: Personify

Title-Subject: [Filtered] Microscopy Sales Opportunity- Bay Area

Message: Good Afternoon!

We have recently had a world leading microscopy client open up a few
microscopy sales opportunities in the greater Bay Area. The ideal
candidate would have an expertise in microscopy and some sales experience.

Please contact me directly to learn more at - md-at-personifysearch.com

Thanks!

Mary Southgate Dickson
Talent Management Executive
Personify
401 Harrison Oaks Boulevard
Suite 350
Cary, North Carolina 27513
www.personifysearch.com
800.875.6188 x 130
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From: microscopy.listserver-at-gmail.com
Date: Wed, 10 Feb 2016 22:42:22 -0600
Subject: [Microscopy] viaWWW:Leitz Wetzlar Orthoplan largefield microscope to give away

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Email: Linda.Davis-at-Vesuvius.com
Name: Linda L Davis

Organization: Vesuvius Refractories R&D USA

Title-Subject: [Filtered] Leitz Wetzlar Orthoplan largefield microscope
to give away

Message: Greetings!!

We have an old but excellent research microscope that has been used
almost daily here since 1969, by the same microscopist who just retired
after 49 years. It is a fantastic scope with a very large footprint.
It is a Leitz Wetzlar Orthoplan, large field microscope. It has been
serviced by professional companies almost yearly since purchased new in
1969.

We are replacing it, but I hate to just throw this away. The oculars,
objectives, accessories may well be something others can use, if not the
entire microscope. It is a museum piece. Again, it has not been
sitting idle. Please let me know if you want the entire microscope
(VERY heavy for shipping, and likely fragile) or any of the parts. It-is
located at our R&D site, halfway between Toledo and Cleveland.


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From: microscopy.listserver-at-gmail.com
Date: Wed, 10 Feb 2016 22:49:16 -0600
Subject: [Microscopy] viaWWW:ESEM live image to external computer

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Email: wambrose-at-unc.edu
Name: Wallace Ambrose

Organization: UNC

Title-Subject: [Filtered] ESEM live image to external computer

Message: Hello Listers
There is a video out BNC on the back of the FEI Quanta 200
ESEM. In Low Vac mode I can see the signal when connecting to an
external view monitor. The signal is horizontal lines that change in
size and number when I change image resolution or scan speed. Also B/C
seems to adjust normally. What can I do to make this into a usable image
signal to use for internet teaching outreach project? Is there a better
way to get the SEM signal to the internet?

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From: vray-at-partbeamsystech.com
Date: Thu, 11 Feb 2016 08:23:25 -0600
Subject: [Microscopy] Re: viaWWW:ESEM live image to external computer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Wallace,

You can use Team Viewer or VNC to directly share desktop of your SEM
with remote computer(s).

If you want to grab video from BNC then decent video grabber card and
some coding/configuring may be needed. I've used Epiphan PCIe in the
past, though not with the FEI E-SEM that you have:

http://www.epiphan.com/products/dvi2pcie-duo/


Valery Ray - also with AIM Lab, UMDCP
=======================================
PBS&T, MEO Engineering Co., Inc.
290 Broadway, Suite 298
Methuen, MA 01844, USA
Phone: +1-978-296-5063
E-Mail: vray-at-partbeamsystech.com
Web: www.partbeamsystech.com
Web: www.freudlabs.com
UMD E-Mail: vray-at-umd.edu

On 2/11/2016 12:05 AM, microscopy.listserver-at-gmail.com wrote:
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} Title-Subject: [Filtered] ESEM live image to external computer
}
} Message: Hello Listers
} There is a video out BNC on the back of the FEI Quanta 200
} ESEM. In Low Vac mode I can see the signal when connecting to an
} external view monitor. The signal is horizontal lines that change in
} size and number when I change image resolution or scan speed. Also B/C
} seems to adjust normally. What can I do to make this into a usable image
} signal to use for internet teaching outreach project? Is there a better
} way to get the SEM signal to the internet?
}
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From: les-at-zsgenetics.com
Date: Thu, 11 Feb 2016 10:48:47 -0600
Subject: [Microscopy] graphene TEM grids

Contents Retrieved from Microscopy Listserver Archives
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Hello,
I am looking for some advice regarding making graphene TEM grids. I've had
some success using a resist-free transfer of graphene from copper foil onto
a grid. It is the very last step that is most vexing: lifting the graphene
covered grid out of the etching solution. The grid is face-down in the
solution, floating on a graphene "raft". Picking it up proves problematic.
The filter paper pick-up method does not work because the surface tension of
the etching solution is such that the grid "runs away" from the paper. I did
grab the grids with tweezers after several tries (same issue), but by then
damage had been done to the monolayer. One paper suggested draining the
etchant away, but then the I am concerned the grid will be face-down on the
bottom of the petri dish.
Any helpful ideas?

Regards,
Larry Scipioni


Regards,
Larry




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From: conversion.seo-at-host2015.lw-china-cdn.com
Date: 13 Feb 2016 03:53:37 +0200
Subject: re: Boost Sales with Social Media Marketing

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The BNC is definitely the video signal coming out of the Quanta. The question for you is how to get the corresponding X and Y coordinate for the line. You should be able to calculate the line time from the number of pixels and dwell time. You know the number of lines from the pixel count. However, I don't think you will be able to determine where the image starts to get it all in sync.

The D connector next to the BNC is for external control as by an EDS system. I don't know the pin-outs offhand but somebody probably knows them. If you don't have such a system, I don't think I would pursue that route.

I would follow Valery's suggestion of using VNC (or Team Viewer) to monitor the existing screen. You will have to deal with port forwarding since FEI tucks their microscope behind their support computer as a firewall. But they basically do what you need as part of their RAPID support system.

Also, remember that F5 will switch between quadrant and full screen mode. Ctrl-F5 will bring the active quadrant up in full screen on the secondary monitor. I recall there is a way to specify to VNC that that screen should be shared with the rest of the world. It may be that the secondary monitor is what you want to share over the net.

Having said all that, be careful. There is a reason that FEI hid the microscope computer behind a firewall. Make sure your VNC viewers are trustworthy and that they are locked down in view-only mode.

Warren Straszheim

-----Original Message-----

Hi Wallace,

You can use Team Viewer or VNC to directly share desktop of your SEM with remote computer(s).

If you want to grab video from BNC then decent video grabber card and some coding/configuring may be needed. I've used Epiphan PCIe in the past, though not with the FEI E-SEM that you have:

http://www.epiphan.com/products/dvi2pcie-duo/

Valery Ray - also with AIM Lab, UMDCP
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} Title-Subject: [Filtered] ESEM live image to external computer
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} There is a video out BNC on the back of the FEI Quanta 200 ESEM. In
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Name: Andrea Kaszas

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Title-Subject: [Filtered] Protocol for embedding rubber in plastic resin

Message: Anybody has a protocol for embedding rubber samples in plastic
resin? Not for cryosectioning.
How to prepare the rubber for it, for example hardening the rubber somehow?
Any suggestion would be greatly appreciated.

Andrea Kaszas
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From: henning.stahlberg-at-unibas.ch
Date: Sat, 13 Feb 2016 04:22:13 -0600
Subject: [Microscopy] ICON 2016 - International Conference on Nanoscopy. Basel, June

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Dear Colleagues,

We would like to draw your attention to the upcoming conference on super-resolution light microscopy, hosted at the University of Basel in Switzerland on June 7-11, 2016.

Join world-changing thinkers and innovators from the Biophysical and Life Science communities:
Super-resolution imaging methods now can provide spatial resolution that is well below the diffraction limit, approaching virtually molecular resolution. They can be applied to biological samples and provide new and exciting views on the structural organization of cells and the dynamics of biomolecular assemblies on wide timescales. These revolutionary developments come with novel requirements for fluorescent probes, labeling techniques, and data interpretation strategies. Researchers from across the scientific spectrum are involved in designing improved tools and solving previously intractable problems using super-resolution techniques.

We invite you to attend the ,AeoInternational Conference On Nanoscopy 2016,Aeo, which will uniquely and exclusively focus on this topic. Held in Basel, Switzerland 07-10 June 2016, it will bring together an international group of experts to discuss the latest advances and future directions in the field.

Markus Sauer, Conference Chair, University of W/orzburg, Germany

Details and registration are on http://www.icon-europe.org

Speakers include
Eric Betzig (Janelia, USA)
Lothar Schermelleh (Oxford University, UK)
Rainer Heintzmann (University Jena, Germany)
Ingo Gregor (University of G/dttingen, Germany)
Thomas Huser (University Bielefeld, Germany)
Markus Sauer (University W/orzburg, Germany)
Adriaan Houtsmuller (Erasmus MC, Netherlands)
Jonas Ries (EMBL, Germany)
Sjoerd Stallinga (TU Delft, Netherlands)
Melike Lakadamyali (ICFO, Spain)
Suliana Manley (EPFL, Switzerland)
Joerg Bewersdorf (Yale, USA)
Katrin Willig (MPI EM, Germany)
Christian Eggeling (Oxford University, UK)
Ilaria Testa (KTH, Sweden)
Martin Booth (Oxford University, UK)
Erik Manders (UvA, Netherlands)
Edoardo Charbon (TU Delft, Netherlands)
Oliver Biehlmaier (University Basel, Switzerland)
and others.

Another 12 presentations will be selected from submitted participant abstracts.

On behalf of the organizing committee,
Gregor Drummen, Oliver Biehlmaier, Henning Stahlberg, and Manuela Holzer

with best greetings,
Henning.

Henning Stahlberg, PhD
Prof. for Structural Biology, C-CINA, Biozentrum, University Basel
Mattenstrasse 26 | D-BSSE | WRO-1058 | CH-4058 Basel | Switzerland
http://c-cina.org | Tel. +41-61-387 32 62



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From: johndmcmaster-at-gmail.com
Date: Sun, 14 Feb 2016 23:58:53 -0600
Subject: [Microscopy] Wanted: Technics Hummer II manual

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Trying to fix one up for personal use. Would be very interested if
someone has a manual or can direct me where I might find one. I'm
currently following a lead with Pumping Station 1 but looking at other
options in case I don't hear back. Thanks!

John


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From: microscopy.listserver-at-gmail.com
Date: Tue, 16 Feb 2016 09:00:28 -0600
Subject: [Microscopy] viaWWW:11th Annual Confocal Microscopy Workshop

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X-from: bob.price-at-uscmed.sc.edu

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Email: bob.price-at-uscmed.sc.edu
Name: Bob Price

Organization: Univ South Carolina

Title-Subject: [Filtered] 11th Annual Confocal Microscopy Workshop

Message: The South Carolina INBRE Program and the USC School of Medicine Instrumentation Resource
Facility are pleased to announce the 11th annual workshop on Basic Confocal Microscopy. The Workshop
will be held June 13-17, 2016 at the USC School of Medicine in Columbia, SC.

Workshop material is directed towards beginning and intermediate users of confocal microscopes and
involves a series of lectures (specimen preparation, labeling strategies, proper set-up of
instrument operating parameters, proper handling of 2D and 3D confocal images in programs such as
Adobe Photoshop and AMIRA), hands on specimen preparation, and time on a number of different point
scanning and spinning disk confocal microscopes.

Companies that have provided equipment and applications experts for past workshops include Leica,
Nikon, Olympus, Perkin Elmer, Zeiss, Intelligent Imaging Innovations (3i) and Photometrix.
Participants will have ample time for hands on use of the instruments and processing of images in
Photoshop and AMIRA during the workshop.

Faculty will include Dr. Jay Jerome of Vanderbilt University, Dr. Ralph Albrecht of the University
of Wisconsin Madison, Dr. John Mackenzie of North Carolina State University, Dr. Tom Trusk of the
Medical University of South Carolina, and Dr. Bob Price of the University of South Carolina.

The $350 tuition covers supplies for processing of specimens, breakfast and lunch for the week, 2
dinners and a reception on Lake Murray.

For registration information please see: http://irf.med.sc.edu/ or contact Anna Harper
(Anna.Harper-at-uscmed.sc.edu) or Bob Price (bob.price-at-uscmed.sc.edu)


Bob Price
Tel: 803-216-3824
Admin Asst Tel: 803-216-3825
Dept Cell Biology and Anatomy
School of Medicine
Univ South Carolina
6439 Garner's Ferry Road
Columbia, SC 29208


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From: bicarbaj-at-mtholyoke.edu
Date: Tue, 16 Feb 2016 14:18:28 -0600
Subject: [Microscopy] LM-Experiences with AmScope?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello everyone,

I am in the process of preparing a grant and I am considering adding
about a dozen compound microscopes with phase contrast capabilities
4x-100x objectives. These microscopes would be used in undergraduate
teaching labs, so high resolution is unnecessary.

I am unfamiliar with the quality of AmScope microscopes but they have
what looks like a decent scope (Model B690B-PL-PCT200INF). We
currently have an army of Olympus BH-2s but the number of biology
majors has increased significantly, so we are looking for some
affordable options.

Does anyone have experience with these microscopes? Any insight would
be greatly appreciated!

Thank you,

-Blanca

-----------------------------------------
Blanca Carbajal Gonzalez, M.S.
Director of Microscopy
Science Center
50 College St
Mount Holyoke College
Clapp Laboratory 123
Office: 413-538-3118
Cell: 559-905-7138
bicarbaj-at-mtholyoke.edu
MHC Microscopy

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7, 38 -- Subject: LM-Experiences with AmScope?
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From: stefan.diller-at-t-online.de
Date: Wed, 17 Feb 2016 06:58:55 -0600
Subject: [Microscopy] Jeol JSM 5900LV manual and remotable functions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,

anybody ou there who can help me out with an operation manual in PDF on the 5900?
I am also looking for a manual concerning the functions which can be remoted on the 5900. Does anybody have experience doing this?
What SEM parameters can be read / written...?


Thanks,
Stefan

--


-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.nanoflight.info
www.stefan-diller.com
www.electronmicroscopy.info
www.elektronenmikroskopie.info
www.zwillingsprojekt.de
www.assisi.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------


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9, 22 -- Subject: Jeol JSM 5900LV manual and remotable functions
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From: tkremer-at-ipstesting.com
Date: Wed, 17 Feb 2016 09:39:15 -0600
Subject: [Microscopy] SEM with EDS Comparing the new benchtop desktop instruments

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello All,

Do any of you have experience with the newer benchtop or desktop SEM's? They seem quite capable and their cost to purchase and maintain, compared to the full scale SEM's, looks attractive. Their advertised performance is appealing. Do they maintain that performance? Do they require much service? Should I have a service contract? Do the accessories function well (e.g. rotating and tilting stage/holder, charge reduction holder)? Is the provided EDS system comprehensive and reliable? Your replies would be greatly appreciated.

Tom Kremer
tkremer-at-ipstesting.com


==============================Original Headers==============================
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From: nizets2-at-yahoo.com
Date: Thu, 18 Feb 2016 04:08:19 -0600
Subject: [Microscopy] bias and gain correction on TEM cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,

we have a SIS Megaview III camera (SIS, then Olympus and now I see it is EMSIS "again") for over 10 years, a real workhorse, always performing well without complaining.
Because it works so well I tend to forget about it and now I am just wondering if I need to update the gain and bias corrections (the correction pictures)?
I can't remember the last time I did it but it is a long time ago.
I know that it is not a lot of work to do it, I just wondered if there is any reason to do this regularly and if yes, what are the time intervals.
Many thanks in advance.

Regards
Stephane

==============================Original Headers==============================
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From: stefan.diller-at-t-online.de
Date: Thu, 18 Feb 2016 04:40:43 -0600
Subject: [Microscopy] Re: bias and gain correction on TEM cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Stephane,
since the blacklevel ("noise") and gain images are a necessary step for getting an artefact-free and evenly background I think you
should do this short procedure (depends what version of Analysis or ITEM software you are using) often.
When I am installing or updating cameras at the customerYens site I ask for the most used magnfication / spotsize / apertures
setting they use and do the correction images with this setting.
But: I also know a customer who is doing these image sets prior to each high-res image he uses for publication (makes sense ;-) ).

Best wishes,
Stefan

-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.nanoflight.info
www.stefan-diller.com
www.electronmicroscopy.info
www.elektronenmikroskopie.info
www.zwillingsprojekt.de
www.assisi.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------

Am 18.02.16 um 11:27 schrieb nizets2-at-yahoo.com:
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} Dear Listers,
}
} we have a SIS Megaview III camera (SIS, then Olympus and now I see it is EMSIS "again") for over 10 years, a real workhorse, always performing well without complaining.
} Because it works so well I tend to forget about it and now I am just wondering if I need to update the gain and bias corrections (the correction pictures)?
} I can't remember the last time I did it but it is a long time ago.
} I know that it is not a lot of work to do it, I just wondered if there is any reason to do this regularly and if yes, what are the time intervals.
} Many thanks in advance.
}
} Regards
} Stephane
}
} ==============================Original Headers==============================
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From: benada-at-biomed.cas.cz
Date: Thu, 18 Feb 2016 05:39:26 -0600
Subject: [Microscopy] Re: bias and gain correction on TEM cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Stephane,
We have SIS MegaView II 16 years old, even older then yours Megaview III.
Well, we used to record the correction images quite frequently, approximately once per month or two. As the ccd chip and scintillator were getting older, the interval for "shading correction" adjustment had to be shorter. After 15 years, we had to record every week a new set of gain and bias images.

You can quite easily check whether you need to perform new adjustment of "shading correction" or not.
1. Take-off the sample holder out of the column
2. Adjust the illumination on the screen
3. Insert camera
4. Adjust beam intensity to ~50%
5. Take an image.
Now measure the histogram of "Gray value distribution" in the recorded image (Main manu: Measure -} Histogram). Look at the course of the histogram line. If it is smooth and the distribution is narrow (~100 for MegaVieew II is OK) then you do not need to adjust "shading correction". Otherwise you should take a new set of gain and bias images.

My best regards

Oldrich


--
Old=oich Benada
Institute of Microbiology CAS, v.v.i.
Laboratory of Molecular Structure Characterization
V/ {} de=ask/* 1083
142 20 Prague 4
Czech Republic

On Thu, 18 Feb 2016 04:19:53 -0600, nizets2-at-yahoo.com wrote :
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} Dear Listers,
}
} we have a SIS Megaview III camera (SIS, then Olympus and now I see it
} is EMSIS "again") for over 10 years, a real workhorse, always
} performing well without complaining. Because it works so well I tend
} to forget about it and now I am just wondering if I need to update
} the gain and bias corrections (the correction pictures)? I can't
} remember the last time I did it but it is a long time ago. I know
} that it is not a lot of work to do it, I just wondered if there is
} any reason to do this regularly and if yes, what are the time
} intervals. Many thanks in advance.
}
} Regards
} Stephane
}
} ==============================Original
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Upozorneni:
Neni-li v teto zprave vyslovne uvedeno jinak, ma tato E-mailova zprava nebo jeji prilohy pouze informativni charakter. Tato zprava ani jeji prilohy v zadnem ohledu ustavy AV CR, v.v.i. k nicemu nezavazuji. Text teto zpravy nebo jejich priloh neni navrhem na uzavreni smlouvy, ani prijetim pripadneho navrhu na uzavreni smlouvy, ani jinym pravnim jednanim smerujicim k uzavreni jakekoliv smlouvy a nezaklada predsmluvni odpovednost ustavu AV CR, v.v.i.

Disclaimer:
If not expressly stated otherwise, this e-mail message (including any attached files) is intended purely for informational purposes and does not represent a binding agreement on the part of Institutes of AS CR. The text of this message and its attachments cannot be considered as a proposal to conclude a contract, neither the acceptance of a proposal to conclude a contract, nor any other legal act leading to concluding any contract; nor it does not create any pre-contractual liability on the part of Institutes of AS CR.



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12, 48 -- From benada-at-biomed.cas.cz Thu Feb 18 05:39:25 2016
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From: WHITTAKS-at-si.edu
Date: Thu, 18 Feb 2016 13:47:18 -0600
Subject: [Microscopy] SEM- spring cleaning- filaments and Amray wehnelt up for grabs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

If anyone is interested I have a spare Wehnelt that was part of an Amray 1810 unit as well as spare tungsten and LaB6 filaments that fit it.

The filaments also fit a Philips XL30 ESEM if an unmodified gun from prior to 2003.

Free but for the cost of shipping....


Scott Whittaker
Manager SEM Lab
Smithsonian Institution
National Museum of Natural History
PO Box 37012** MRC104
Washington DC 20013-7012
202-633-0891



==============================Original Headers==============================
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From: microscopy.listserver-at-gmail.com
Date: Thu, 18 Feb 2016 18:34:58 -0600
Subject: [Microscopy] viaWWW:How batch convert emi to other format

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Email: 12hy1-at-queensu.ca
Name: Hongbing Yu

Organization: Queen's University at Kingston

Title-Subject: [Filtered] How batch convert emi to other format

Message: Dear all,
We have an FEI TEM microscope. The STEM images Acquired in TIA software are saved in emi format. I
can open them in TIA software, and can only export them one by one. That is quite annoying. Is there
anybody who has any idea how to batch convert the images?

Thanks
Hongbing Yu


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From: microscopy.listserver-at-gmail.com
Date: Thu, 18 Feb 2016 18:35:55 -0600
Subject: [Microscopy] viaWWW:Attn: LaB6 cathode/e-gun mfrs and vendors

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Email: mlibbee-at-lbl.gov
Name: Marissa Libbee

Organization: LBL

Title-Subject: [Filtered] Attn: LaB6 cathode/e-gun mfrs and vendors

Message: I'm interested in tracking down mfrs and vendors of anything LaB6 related. Please contact
me offline with company names and preferably, a contact name within the company. Thanks!

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From: microscopy.listserver-at-gmail.com
Date: Thu, 18 Feb 2016 18:37:18 -0600
Subject: [Microscopy] viaWWW:EM Connectome Annotation Team Manager (362-908 )

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Email: murphyv-at-janelia.hhmi.org
Name: Veronica Murphy

Organization: HHMI Janelia Research Campus

Title-Subject: [Filtered] EM Connectome Annotation Team Manager (362-908 )

Message: The Janelia Research Campus of the Howard Hughes Medical Institute (Janelia) is using
high-throughput electron microscopy (EM) to completely image multiple brains and nervous systems of
the fruit fly, Drosophila melanogaster. Janelia scientists will utilize these massive data sets to
reconstruct neuronal circuits, ultimately culminating in the complete connectome-oor wiring
diagram-oof the fly brain. The connectome will provide the most complete anatomical description of a
complex nervous system to date in an organism uniquely suited to combine this anatomical information
with functional imaging, electrophysiology, quantitative behavioral assays, precise genetic
manipulation of identified cell types and computational modeling. Thus we expect these circuit-level
reconstructions to inform specific biological experiments, enabling dramatic leaps in our
understanding of brain function.

Janelia is seeking a talented individual to assemble, train, and lead a group of approximately ten
neuron tracing staff, with potential for a significantly larger effort based on the established
success of the initial team. In order to be successful, the manager must have research experience in
neuroanatomy, preferably in the application of electron microscopy to elucidate neurobiological
function. Knowledge of Drosophila biology and/or software for reconstructing objects from serial
section images is strongly desired. The manager will collaborate with scientists (at Janelia and
elsewhere) whose research is targeted toward understanding of specific circuits and functions in the
fly brain, guiding their team of highly trained specialists to identify, trace, and annotate
specific collections of neurons from the vast data being produced. The manager will also collaborate
closely with the project teams producing the EM data and developing the algorithms and tools that
enable the work of their team. Thus, the ideal candidate for this position must have a neuroscience
background, the ability to teach scientific concepts, and experience in supervising and leading the
work of others in a dynamic setting. The candidate must further possess an innate sense of pace and
urgency, and excel at working in a team science environment to achieve challenging goals,
prioritizing multiple assignments, and communicating clearly to a broad group of collaborators.
Experience in supervising and leading the work of others is desired but not strictly required.

HHMI is an Equal Opportunity Employer.

Please apply online at
https://hhmi-openhire.silkroad.com/epostings/index.cfm?fuseaction=app.jobinfo&jobid=362&company_id=16908&version=1&source=ONLINE&jobOwner=992274&aid=1.


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From: wim.hagen-at-me.com
Date: Thu, 18 Feb 2016 20:53:36 -0600
Subject: [Microscopy] Re: viaWWW:How batch convert emi to other format

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Hongbing,

TIA folder export:
- Open menu Tools\Components.
- Click ,AeuInstall,Aeu.
- Type ,Aeufolderexport.wsc,Aeu.
- ,Aeuok".
Now that this component is installed, there will be an additional menu in the processing tasks.
Check settings and hit Export.

Best,

Wim Hagen
EMBL Heidelberg

} On Feb 19, 2016, at 1:58 AM, microscopy.listserver-at-gmail.com wrote:
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} Email: 12hy1-at-queensu.ca
} Name: Hongbing Yu
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} Organization: Queen's University at Kingston
}
} Title-Subject: [Filtered] How batch convert emi to other format
}
} Message: Dear all,
} We have an FEI TEM microscope. The STEM images Acquired in TIA software are saved in emi format. I
} can open them in TIA software, and can only export them one by one. That is quite annoying. Is there
} anybody who has any idea how to batch convert the images?
}
} Thanks
} Hongbing Yu
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From: john.mitchels-at-gmail.com
Date: Fri, 19 Feb 2016 08:15:12 -0600
Subject: [Microscopy] Consensus on UA replacement stains

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers

I am need of some experience here. Has anyone played with UA
replacements such samarium and gadolinium triacetate for en bloc
staining? How does this compare in performance, permeation,
precipitation etc? Is it an acceptable alternative for all tissues or
just selected ones. I am aware of many papers on the mater, and my own
dabbling but I tend to find the microscopy list opinion a little
easier to swallow.

Thanks in advance
J

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From: hlowers-at-usgs.gov
Date: Fri, 19 Feb 2016 14:33:38 -0600
Subject: [Microscopy] EPMA 2016 Topical Conference and Student Support

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Announcing a MAS Topical Conference, Electron Probe Microanalysis 2016
May 16-19, 2016 at the University of Wisconsin-Madison


TOPICAL CONFERENCE PROGRAM
-Practical microanalysis for beginners to professionals
-Plenary Conference including user meetings, tutorials, group
discussion, problem solving, and instrument demos
-Quantitative analysis using the electron microprobe and SEM with EDS and WDS
-Improvements in microanalysis from measurement to accuracy
-Trace element analysis
-Compositional x-ray mapping and cathodoluminescence
-Applications in earth science, materials science, and industry

STUDENT FINANCIAL SUPPORT AVAILABLE
-Early career scholars: undergraduate, graduate, postdoc, and early
career professionals may apply
-Priority will be given to those with co-sponsorship from a
university, advisor, or employer
-ECS awardees may submit a 2-page abstract for platform or poster presentation.
-Awardees will receive a maximum of $750 reimbursement for travel,
lodging, and registration.


Please visit for more information
http://www.microprobe.org/topical-conferences/epma-2016-1/epma-2016


Heather Lowers, EPMA 2016 ECS Coordinator
Paul Carpenter, EPMA 2016 Planning Chair

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From: dblackburn2000-at-yahoo.com
Date: Sat, 20 Feb 2016 10:55:20 -0600
Subject: [Microscopy] question - Allergy to histological solvents?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello

After decades of using standard organic solvents for paraffin- section histology, I find that I've become highly allergic to the fumes.-* These include commercial preps like Citrosolv and Hemo-De, as well as xylene and toluene, which cause dizziness and pounding headaches.-* This has made staining and coverslipping very difficult, even with-* hood.-*-*-*Can anyone recommend alternative solutions for (a) dewaxing and (b) coverslipping with Permount?-* thanks very much!

Daniel Blackburn
Biology, Trinity College (Hartford CT)
-*


==============================Original Headers==============================
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From: oshel1pe-at-cmich.edu
Date: Sun, 21 Feb 2016 09:20:14 -0600
Subject: [Microscopy] question - Allergy to histological solvents?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Daniel,

I assume you're doing these steps in a fume hood. One thing we've found for people who are sensitive to solvent fumes are charcoal-lined dust masks. Search amazon.com for "charcoal filter masks". We use the 3M type (but NOT for the prices here - we got ours through Fisher Scientific).
So far, they've worked fine.

Looks like you've already tried the various xylene replacements.

Phil

Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

________________________________________
X-from: dblackburn2000-at-yahoo.com [dblackburn2000-at-yahoo.com]
Sent: Saturday, February 20, 2016 12:16 PM
To: Oshel, Philip Eugene

Hello

After decades of using standard organic solvents for paraffin- section histology, I find that I've become highly allergic to the fumes. These include commercial preps like Citrosolv and Hemo-De, as well as xylene and toluene, which cause dizziness and pounding headaches. This has made staining and coverslipping very difficult, even with hood. Can anyone recommend alternative solutions for (a) dewaxing and (b) coverslipping with Permount? thanks very much!

Daniel Blackburn
Biology, Trinity College (Hartford CT)



==============================Original Headers==============================
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From: microscopy.listserver-at-gmail.com
Date: Sun, 21 Feb 2016 14:53:15 -0600
Subject: [Microscopy] viaWWW:Job Opportunity: Senior Analytical Technologist

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Email: q98-at-daycommunications.ca
Name: Human Resources

Organization: Ontario Ministry of the Environment and Climate Change

Title-Subject: [Filtered] Job Opportunity: Senior Analytical Technologist

Message: Erase Senior Analytical Technologist
Are you a flexible and versatile chemist with passion for investigating a wide range of inorganic,
particulate and organic substances?
If so, bring your knowledge and skills to the Ministry of the Environment and Climate Change, to
support environmental programs through monitoring, emergency response and litigation.
What can I expect to do in this role?
In this role, you will:
-i carry out moderate to highly complex analysis and identification of inorganic and organic material
in a wide array of environmental matrices
-i coordinate workload within the group and provide leadership to junior technologists to ensure
turn-around time is met
-i implement new and improved methods for the preparation, analysis and reporting of sample analysis
-i provide joint problem solution with customers and colleagues in the response to non-routine
environmental investigations
Location: Etobicoke
How do I qualify?
Knowledge of Laboratory Equipment and Systems:
-i You have current knowledge of and practical experience with the development, commissioning,
maintenance, and repair of advanced automated analytical laboratory instrumentation, including
optical and electron microscopy, x-ray fluorescence, x-ray diffraction, infra-red and UV-VIS
spectroscopy
-i You have practical experience with information management systems and their relationship to
laboratory data processing to ensure that data are correctly stored in the database and transmitted
to clients
-i You can operate, maintain and troubleshoot automated and semi-automated equipment
Knowledge of Sample Preparation:
-i You have knowledge of and practical experience with inorganic and organic analytical chemistry,
particulate analysis chemistry and identification methods related to a variety of environmental
sample matrices, including water, sewage, sediments, soil and air filters
-i You can use your knowledge to utilize laboratory methods that provide complementing types of data
-i You have knowledge of sampling methods and sample preservation procedures to provide advice
regarding proper sampling and sample preservation
Communication and Interpersonal Skills:
-i You have good oral and written communication and interpersonal skills
-i You are able to provide guidance to technologists and clients and prepare client reports and
document methods
-i You can communicate and confer with customers, including establishing data quality objectives,
interpreting data, joint problem solution and education of customers
-i You can provide factual testimony as to accuracy and precision of results, technology functions,
limitations and other qualities in court during legal proceedings
-i You are able to provide guidance, oversee work of junior staff and train others
-i You are capable of establishing effective working relationships with scientific, technical,
administrative, management co-workers and a diverse customer community
Analytical and Evaluative Skills:
-i You can select standard or alternative testing procedures, including both quantitative or
semi-quantitative methods and qualitative identification
-i You can troubleshoot problems related to sampling, testing and analysis of unusual samples
-i You can assess data quality against established criteria or in the absence of established criteria
against accepted scientific principles
-i You confer with publications, internet as well as external and external experts when confronted
with unusual samples
-i You know the principles of quality control and assurance, including ISO/IEC 17025
-i You know and can apply statistics to evaluate viability of new analytical methods
Salary Range: $1,216.18 - $1,443.88 per week
Additional information:
-i 1 Permanent, 125 Resources Rd, Etobicoke, Toronto Region
Please apply online, only, at www.ontario.ca/careers, quoting Job ID 89210, by Friday, March 4,
2016. Please follow the instructions to submit your application. Faxes are not being accepted at
this time.
If you require accommodation in order to participate in the recruitment process, please contact us
at www.gojobs.gov.on.ca/ContactUs.aspx to provide your contact information. Recruitment Services
staff will contact you within 48 hours. Only those applicants selected for an interview will be
contacted.
The Ontario Public Service is an inclusive employer. Accommodation will be provided in accordance
with Ontario-is Human Rights Code.
www.ontario.ca/careers
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From: microscopy.listserver-at-gmail.com
Date: Mon, 22 Feb 2016 06:04:06 -0600
Subject: [Microscopy] viaWWW:Cambridge S360 SEM Computer Error

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Hi Daniel,

A serious problem.
By listing, I have come with suggestions.
I have long used a Gum Damar formulation that I cannot offer, because I have prepared it in xylene.

NOTE: for those who might be interested - or users of Damar - I have a liter that I will NEVER use more than a few ml's over my reasonably extended age - now 76. Further, I don't do paraffin any more.

The suggestions:

If you can arrive at a solvent for Damar that is miscible with xylene/toluene, then it would be possible that you could prepare your own Damar. NOTE: Damar applied in the 1960's still have no sign of drying/cracking/crystalizing.
Source: http://www.eco-house.com/shop/950-damar-resin-crystals-graded/

You might test a hydrophilic mountant.
Source: https://www.emsdiasum.com/microscopy/products/histology/mounting_media.aspx

If your allergy is broad-band, then you may have to resort to the latter. If it is more specific - just for those you mentioned - then your options might include Canada Balsam that is very natural, but also often found with benzene or xylene or toulene. I have some that will never be used as it requires some solvent that might not raise a sneeze.

Email me directly if you wish to discuss further,

Frederick C Monson, PhD
Center for Microanalysis and Imaging, Research and Training (CMIRT)
West Chester University of Pannsylvania
West Chester, PA, 1938
610-738-0437
fmonson-at-wcupa.edu

________________________________________
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Email: mp76ers-at-gmail.com
Name: Mojahed

Organization: MERC

Title-Subject: [Filtered] Cambridge S360 SEM Computer Error

Message: Hi to all,
I have a problem with the microcomputer (SBC) of an old s360 SEM. It's model is PME 68-2 (or PME
68-12). It has s1-s8 LEDs on its front panel for error checking. Now s5 LED indicate en error. Does
anyone have its manual or know what does it mean?

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From: tbargar-at-unmc.edu
Date: Mon, 22 Feb 2016 08:30:10 -0600
Subject: [Microscopy] Users of FEI TeneoVS SEM

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers:

I would like to hear from anyone out there who is using FEI's TeneoVS SEM. I want to know your experiences with it and I would like to get a sample run as a demo, we can cover any associated costs.

Tom Bargar
UNMC
Electron Microscopy Core Facility
986395 Nebraska Medical Center
Omaha, NE 68198-6395
402-559-7347
tbargar-at-unmc.edu


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From: Winston.Wiggins-at-cshs.org
Date: Mon, 22 Feb 2016 10:09:23 -0600
Subject: [Microscopy] RE: question - Allergy to histological solvents?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Unfortunately, you,Aeove probably lost your sense of smell and taste if you,Aeove become that sensitized. I would suggest the quickest remedy may be using a half-mask respirator with organic vapor filter cartridges, even though you may be using a fume hood. I think paint respirators may work since they filter organics/solvents/paints. Make sure it fits correctly too. It won't do to have a proper filtering assembly if it doesn't fit correctly!
Good luck.
~Winston

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Winston W. Wiggins, E.M. Pathology Specialist
CEDARS-SINAI MEDICAL CENTER, Pathology & Lab Medicine
Electron Microscopy, LLSPT, A828
8700 Beverly Blvd.
Los Angeles, CA 90048-1865
OAess310-423-1363 (off); 310-423-5323 (lab); 310-423-0685 (fax)
Winston.Wiggins-at-CSHS.org
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~


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From: frank_karl-at-ardl.com
Date: Wed, 24 Feb 2016 07:07:25 -0600
Subject: [Microscopy] Fiber and Fabric

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Mississippi State University's College of Engineering will be hosting a Research Experience for Undergraduates May 31-Aug. 6.

Research will be in Materials Science and Engineering and will include topics such as biomaterials, nanoparticles, composites & polymers, and nanocrystalline materials. The program will also include professional and development seminars, industry site visits, and GRE preparation workshops.

The REU includes a weekly stipend, on-campus housing, and a meal allowance. For more information and/or to apply on-line please visit the college of engineering website: www.bagley.msstate.edu/REU/.

Amanda Lawrence
Outreach Coordinator/Research Associate
Institute for Imaging and Analytical Technologies
Mississippi State University




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From advertise.bz222dcedu-at-gmail.com Wed Feb 24 00:57:18 2016
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Message-ID: {2EC237BE.15C3727C-at-gmail.com}

In 1921, Ms.Valencia Arton took for her second term, summer quarter a course called Textiles at the University of Chicago. She constructed a lab book consisting of experiments, typed and long hand notes and samples of fabric before and after testing.

I've found her notebook over 30 years ago in a used bookstore thinking, "What a source of old fabric samples and fibers" but I never did anything with it.

I'm sure Ms. Arton is long past caring, but I can't help but think some microscopist could find a publication in these pages, if they look hard enough. Valencia would probably enjoy that.

The book is free to anyone who would want it, no strings attached. She does mention the microscope and it is an interesting look at the analytical fiber and fabric procedures of 1921.

Contact me if you have question or need more detail.........

Frank
ARDL


This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc.



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From: apamatos-at-gmail.com
Date: Wed, 24 Feb 2016 08:08:12 -0600
Subject: [Microscopy] Extension of the deadline for abstract submission to Ultrapath XVIII

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues,

The Organizing Committee of Ultrapath XVIII
(http://congress.ultrapathxviii.org) has extended the deadline for
abstract submission to March 24, 2016.
Your work is very important for the Society and your contribution to the
Congress will help all of us to keep improving and spread the word about
the continued importance of Ultrastructural Pathology.

With kind regards,

From the Organizing Committee,

A.P. Alves de Matos
Chair of UltrapathXVIII


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From: ph2-at-sprynet.com
Date: Wed, 24 Feb 2016 09:21:30 -0600
Subject: [Microscopy] Call for Papers - Inter/Micro 2016

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Call for Papers - Register Online Today

Inter/Micro 2016

http://www.mcri.org/v/101/intermicro

An international microscopy conference.
June 6 - 10 at McCrone Research Institute, Chicago


Join some of the world's leading amateur and professional microscopists for
the 68th annual Inter/Micro conference, featuring:
. Research presentations on techniques/instrumentation,
industrial/environmental microscopy, and chemical/
forensic microscopy
. Two-day workshop on microscopy of sand, taught by Thomas J. Hopen of the
ATF
. Evening with Brian Ford presentation: "Magical Mystery Tour of the Cell"
. State Microscopical Society of Illinois Awards Dinner
. ... and more! View the schedule of events.

Research presentations will be held June 6 - 8. Speakers will receive a $50
registration discount. Read abstract submission guidelines.

Use our secure online registration form at www.mcri.org to reserve your seat
today.

We look forward to seeing you in Chicago!


McCrone Research Institute
A Not-for-Profit Corporation
2820 South Michigan Avenue, Chicago, IL 60616-3230
Phone: 312-842-7100 Fax: 312-842-1078
www.mcri.org
intermicro-at-mcri.org

.........................................
Andrew Anthony "Tony" Havics, CIH, PE
Environmental, Health & Safety, Microscopy, Materials Science & Forensic
Engineering
pH2, LLC
5250 E US Highway 36, Suite 830
Avon, IN 46123
(317) 718-7020 Office
(317) 718-7038 Fax
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From: tbargar-at-unmc.edu
Date: Wed, 24 Feb 2016 11:15:11 -0600
Subject: [Microscopy] Free Denton Vacuum Evaporator DV-502

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We are getting rid of our Denton Vacuum Evaporator DV-502. If anyone out there would like a free vacuum evaporator you are welcome to come and get it. Our lab is located at UNMC in Omaha, Nebraska.

Tom Bargar
UNMC
Electron Microscopy Core Facility
986395 Nebraska Medical Center
Omaha, NE 68198-6395
402-559-7347
tbargar-at-unmc.edu


The information in this e-mail may be privileged and confidential, intended only for the use of the addressee(s) above. Any unauthorized use or disclosure of this information is prohibited. If you have received this e-mail by mistake, please delete it and immediately contact the sender.



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From: tbargar-at-unmc.edu
Date: Thu, 25 Feb 2016 10:27:11 -0600
Subject: [Microscopy] would like to hear from Tescan customers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear listers,

I would like to hear privately from anyone using Tescan SEM and especially anyone who is using a Tescan with the integrated Gatan ultramicrotome for serial block face imaging. I'm not familiar with this company, so all feedback would be appreciated.

Tom Bargar
UNMC
Electron Microscopy Core Facility
986395 Nebraska Medical Center
Omaha, NE 68198-6395
402-559-7347
tbargar-at-unmc.edu


The information in this e-mail may be privileged and confidential, intended only for the use of the addressee(s) above. Any unauthorized use or disclosure of this information is prohibited. If you have received this e-mail by mistake, please delete it and immediately contact the sender.



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From: Z.Zhou-at-lboro.ac.uk
Date: Fri, 26 Feb 2016 08:23:05 -0600
Subject: [Microscopy] Microscopy facility online booking systems

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Dear Fellow Microscopists,

What are the typical software systems you use to manage on-line booking to use scopes or sample prep facilities? Either commercially available or free systems are of my interest. I would be extremely grateful if you would please share with me the experiences and expectations you have had.
The system we use now allows on-line booking of a dozen resources from users, notifying users in advance, managing the cost of the usage automatically, etc. However, it's not embedded within individual equipment. The booking and the usage is not electronically linked. Ideally when a booking is made to use a scope, and this user account will implement the booking by logging in the facility on the session. Therefore the booking and the real usage will be directly linked with a true picture. Anybody is using such a system now?

Best regards,
Dr Z Zhou
Loughborough University, UK


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From: henning.stahlberg-at-unibas.ch
Date: Sat, 27 Feb 2016 03:26:19 -0600
Subject: [Microscopy] PostDoc in cryo-EM and image processing in Basel, Switzerland

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A PostDoc position is available in the BioEM Lab of the University of Basel, Switzerland.

We are looking for a person with experience in single particle cryo-EM and image processing, using RELION, FREALIGN, EMAN2, and other packages. The position is available immediately, and funded initially for two years, with the possibility for multi-year extension and possible transition into a permanent position. The role of this person should be to perform cryo-EM sample preparation and cryo-EM data collection, and perform advanced image processing and 3D reconstructions. Expertise in single particle image analysis is required.
Several high-impact biological projects are waiting, including studying larger membrane protein complexes by cryo-EM as single particles, as well as helical assemblies.

The person should serve as liaison between the BioEM Lab, which is a newly established high-resolution cryo-EM service facility at the the Center for Cellular Imaging and NanoAnalytics of the Univeristy of Basel, and the laboratory of biomolecular research at the Paul-Scherrer Institute (PSI) in Villigen. An interaction with collaborators and clients from the pharmaceutical industry interested in membrane protein structure determination is also foreseen.

This position involves high-resolution cryo-EM structural work on membrane proteins, for which our FEI Titan Krios (Quantum-LS GIF / K2 Summit) and the FEI Polara (K2 Summit) are outstanding instruments. We also operate a number of additional instruments for sample screening and cryo-EM grid analysis, including a FEI Talos, CM200FEG, T12, CM100, CM10, Versa3D, and others. Large computer clusters are available. The lab atmosphere is especially nice, and the city of Basel in Switzerland at the border to Germany and France has a beautiful historic center and a rich culture, and provides a high standard of living. This is a good place to live and to do science.

If you are interested, please contact Henning.Stahlberg-at-unibas.ch for further infos.


Henning Stahlberg, PhD
Prof. for Structural Biology, C-CINA, Biozentrum, University Basel
Mattenstrasse 26 | D-BSSE | WRO-1058 | CH-4058 Basel | Switzerland
http://c-cina.org | Tel. +41-61-387 32 62




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From: microscopy.listserver-at-gmail.com
Date: Sat, 27 Feb 2016 17:36:16 -0600
Subject: [Microscopy] viaWWW:Biological X-ray Analysis with SEM EDS

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Email: htowbin-at-amnh.org
Name: Henry Towbin

Organization: American Museum of Natural History

Title-Subject: [Filtered] Biological X-ray Analysis with SEM EDS

Message: Hi All,
Can anyone provide some referenced for good biological thin film x-ray
analysis? I am particularly interested in how to prepare good standards
for using the Hall-Marshal or similar method to analyze C, N and P.
I am trying to help some colleague with analyzing the compositions of
singles celled organisms with x-ray analysis in an SEM.
They are attempting to dry the cells on a formvar support and plan to
measure counts from the cell and the film and then subtract the counts
of the film to calculate composition. I am highly skeptical of the
methods they want to use from Norland et al. 1995 (Light Element
Analysis of Individual Bacteria by X-Ray Microanalysis), so any advice
would be much appreciated.
Thank you,
Henry

Norland et al. 1995, Light Element Analysis of Individual Bacteria by
X-Ray Microanalysis, http://aem.asm.org/content/61/4/1357.full.pdf
______________________________
Henry Towbin
Laboratory Co-manager
Microscopy and Imaging Facility
American Museum of Natural History
79th St & Central Park West
NY, NY 10024

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From: hlowers-at-usgs.gov
Date: Mon, 29 Feb 2016 15:59:48 -0600
Subject: [Microscopy] EPMA2016 Topical Conference and Student Support

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Announcing a MAS Topical Conference, Electron Probe Microanalysis 2016
May 16-19, 2016 at the University of Wisconsin-Madison

STUDENT FINANCIAL SUPPORT AVAILABLE!

TOPICAL CONFERENCE PROGRAM
-Practical microanalysis for beginners to professionals
-Plenary Conference including user meetings, tutorials, group
discussion, problem solving, and instrument demos
-Quantitative analysis using the electron microprobe and SEM with EDS and WDS
-Improvements in microanalysis from measurement to accuracy
-Trace element analysis
-Compositional x-ray mapping and cathodoluminescence
-Applications in earth science, materials science, and industry

STUDENT FINANCIAL SUPPORT AVAILABLE
-Early career scholars: undergraduate, graduate, postdoc, and early
career professionals may apply
-Priority will be given to those with co-sponsorship from a
university, advisor, or employer
-ECS awardees may submit a 2-page abstract for platform or poster presentation.
-Awardees will receive a maximum of $750 reimbursement for travel,
lodging, and registration.

DEADLINE MARCH 15, 2016 FOR ABSTRACTS AND ECS APPLICATIONS

Please visit for more information
http://www.microprobe.org/topical-conferences/epma-2016-1/epma-2016

Heather Lowers, EPMA 2016 ECS Coordinator
Paul Carpenter, EPMA 2016 Planning Chair

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From: microscopy.listserver-at-gmail.com
Date: Thu, 12 May 2016 07:00:52 -0500
Subject: [Microscopy] Administrivia: Nestor is doing testing Ignore this message

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Sorry All

Must do a bit of testing to restore functionality which
has broken.

Nestor



--
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From: yaroslav.tsybovsky-at-nih.gov
Date: Thu, 12 May 2016 12:04:23 -0500
Subject: [Microscopy] TEM: Research Associate position at Leidos Biomedical Research,

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues,

The Electron Microscopy Laboratory at the Frederick National Laboratory for Cancer Research, located in Frederick, Maryland, has an open position of Research Associate I. Main duties will include: 1) biological sample preparation for electron microscopy (negative staining, plastic embedding, vitrification for cryo-EM, other approaches as necessary), 2) operation of electron microscopes for imaging and data collection, and 3) analysis and presentation of data. If interested, please apply directly using the following link:

http://jobs.leidos.com/ShowJob/Id/832108/Research-Associate-I-628272-%28NCI%29/

Best regards,

Yaroslav Tsybovsky (Contractor)
Scientist
Frederick National Laboratory for Cancer Research
Leidos Biomedical Research, Inc.
Post Office Box B
Frederick, Maryland 21702



==============================Original Headers==============================
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7, 47 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
7, 47 -- Subject: TEM: Research Associate position at Leidos Biomedical Research,
7, 47 -- Inc./Frederick National Laboratory for Cancer Research
7, 47 -- Thread-Topic: TEM: Research Associate position at Leidos Biomedical
7, 47 -- Research, Inc./Frederick National Laboratory for Cancer Research
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From: yaroslav.tsybovsky-at-nih.gov
Date: Thu, 12 May 2016 12:04:23 -0500
Subject: [Microscopy] TEM: Research Associate position at Leidos Biomedical Research,

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues,

The Electron Microscopy Laboratory at the Frederick National Laboratory for Cancer Research, located in Frederick, Maryland, has an open position of Research Associate I. Main duties will include: 1) biological sample preparation for electron microscopy (negative staining, plastic embedding, vitrification for cryo-EM, other approaches as necessary), 2) operation of electron microscopes for imaging and data collection, and 3) analysis and presentation of data. If interested, please apply directly using the following link:

http://jobs.leidos.com/ShowJob/Id/832108/Research-Associate-I-628272-%28NCI%29/

Best regards,

Yaroslav Tsybovsky (Contractor)
Scientist
Frederick National Laboratory for Cancer Research
Leidos Biomedical Research, Inc.
Post Office Box B
Frederick, Maryland 21702



==============================Original Headers==============================
7, 47 -- From yaroslav.tsybovsky-at-nih.gov Thu May 12 12:04:23 2016
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7, 47 -- From: "Tsybovsky, Yaroslav (NIH/NCI) [C]" {yaroslav.tsybovsky-at-nih.gov}
7, 47 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
7, 47 -- Subject: TEM: Research Associate position at Leidos Biomedical Research,
7, 47 -- Inc./Frederick National Laboratory for Cancer Research
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7, 47 -- Research, Inc./Frederick National Laboratory for Cancer Research
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From: microscopy.listserver-at-gmail.com
Date: May 12, 2016 at 7:00:52 AM CDT
Subject: [Microscopy] Administrivia: Nestor is doing testing Ignore this message

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Sorry All

Must do a bit of testing to restore functionality which
has broken.

Nestor



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From: microscopy.listserver-at-gmail.com
Date: May 12, 2016 at 5:58:02 AM CDT
Subject: [Microscopy] Last announcement - Abstract submission deadline extended to 18th May 2016 43rd SCUR-The SKIN IMAGING SOCIETY-Annual Meeting, LYON,29-30th August 2016

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Note: SCUR Annual meeting will take place during the first 2 days of
EMC16 (the latter will take place Aug, 28 - Sept, 2 in Lyon, France)


Interested in Skin Research - Ultrastructure -Morphology - Imaging techniques and other modern
techniques in Dermatological / Dermatopathological Research & Science?


Dear Colleague(s),

It is still NOT TOO LATE to submit your abstract for presentation at the forthcoming
43rd Annual meeting of SCUR- the Skin Imaging Society, 29th-30th August 2016 in Lyon, France [cf. www.scur.org]

New deadline: May 18th 2016

NOTE: 4 Travel Grants of 200[euro] for junior scientists (students and researchers under 35 y. o.)
and technicians, members of SCUR
(either active members or prospective new members submitting SCUR membership application simultaneously).

THREE Awards
*Best young scientist presentation (200 [euro])
*Best oral presentation (200 [euro])
*Best poster presentation (200 [euro])

Please, send your abstract to { { scur.lyon2016-at-gmail.com } }
(find RTF form on website www.scur.org - and link then to
'Next SCUR Meeting' and find
General information at: https://www.scur.org/content/e1174/e1181/e1182 ,

at the bottom of that page also: all forms
[Announcement = Invitation to Lyon - Society for Cutaneous Ultrastructure Research,
{abstract submission form} , {Social Events Registration Form} ,
{Membership Application Form} as well as the { TRAVEL GRANT APPLICATION FORM} ]

You can visit / attend SCUR sessions if you register for whole EMC-16 - or you register only for SCUR program.
(BUT: 'Registering' also for {SCUR only} must be done via the EMC-16-Website
= http://www.emc2016.fr/en/register/fees - Early Bird Registering until 1 June 2016 -
FIND there (if you only want to attend the SCUR-Meeting) also the rate for:

"2 Days SCUR session only (29th & 30th August) [at a special reduced rate of ] [euro] 250 " )


End your summer vacation with an exciting stop at the { Gaules capital of Lugdunum} :
so many things to see and to do, apart from the scientific sessions, at the heart of the
{Beaujolais region} and the { famous French kitchen} !
(visit: http://www.onlylyon.com/en/ )


SCUR 2016 ALL INFORMATION:


Thanking you for support and further distribution of this message to other interested colleagues/persons.

Looking forward to meeting YOU and many other interested Colleagues there,


With best regards,

Wolfgang MUSS (also Member of MSA)
Secretary of SCUR - The Skin Imaging Society
SALZBURG-AUSTRIA





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From: microscopy.listserver-at-gmail.com
Date: May 11, 2016 at 7:39:03 PM CDT
Subject: [Microscopy] viaWWW:Job Opening at UCSB: TEM Engineer

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Email: ucsb.materials-at-gmail.com Name: Dawn McTague

Organization: University of California, Santa Barbara

Title-Subject: [Filtered] Job Opening at UCSB: TEM Engineer

Message: The University of California Santa Barbara Materials Department has an immediate opening
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to maintenance and upkeep of advanced equipment. Incumbent must have a strong background in
research microscopy with a high level of expertise in TEM microscopy. Experience working in a
fast-paced research environment with a diverse population combined with an ability to develop
thorough training programs is required. Ph.D. preferred.

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From: microscopy.listserver-at-gmail.com
Date: May 11, 2016 at 6:30:26 AM CDT
Subject: [Microscopy] Workshop announcement: Electron crystallography of 2D crystals of

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

Sorry, wrong dates: August 22-27, 2016. (Lectures August 23-26).

Henning.

Henning Stahlberg, PhD
Prof. for Structural Biology, C-CINA, Biozentrum, University Basel
Mattenstrasse 26 | D-BSSE | WRO-1058 | CH-4058 Basel | Switzerland
http://c-cina.org | Tel. +41-61-387 32 62



On 11 May 2016, at 10:49, Henning Stahlberg {henning.stahlberg-at-unibas.ch} wrote:

Dear colleagues,

We are happy to announce the fifth 2DX Workshop on Electron Crystallography of Membrane Proteins, which will take place at the University of Basel (Switzerland), on August 22-27, 2016. The workshop will provide hands-on training on practical aspects of 2D crystal data collection on a Titan Krios, and cover in depth the theoretical foundations and the practical image processing of 2D crystal cryo-EM images with the 2DX software package. No previous experience is required.

Topics include:
Basics of 2D electron crystallography
* Introduction to 2D electron crystallography
* Cryo-electron microscopy sample preparation for 2D crystals of membrane proteins
* Cryo-EM data collection of 2D crystals
* Introduction to 2DX
* Drift correction using ZORRO
Basic image processing with 2DX
* Determination of the defocus, crystal tilt geometry, crystal lattice
* Crystal Unbending - including movie-mode drift correction of individual unit cells in dose-fractionated image series
* 2D merging and 3D merging with reconstruction via weighted back projection
* Automation of the processing pipeline
Advanced topics in image processing with 2DX
* Approaches for a partial reconstruction of amplitudes and phases inside of the missing cone
* Processing of 2D crystal unit cells with RELION and FREALIGN, under exploitation of neighborhood correlation of certain image parameters
* Advanced usage of the 2DX backend library
* Preparation of final results with quantitative description of statistical values (e.g., resolution) for export to databases (EMDB).

Computing infrastructure will be provided in place and participants are welcome to bring their own data sets and/or computer (OSX or Linux). More information can be found at
http://www.2dx.unibas.ch/workshop/2016

For registration, please include information about your affiliation, and a short motivation statement (max 200 words).
The registration fee is 250 CHF (travel and accommodation is not included), registration deadline is June 30th. As we can only provide a limited number of workstations, we typically accept participants on a "first come - first serve" basis. Please do not hesitate to contact us for any questions. Looking forward to welcoming you in Basel,

Nikhil Biyani,
Ricardo Righetto,
Robert McLeod,
Henning Stahlberg.

Henning Stahlberg, PhD
Prof. for Structural Biology, C-CINA, Biozentrum, University Basel
Mattenstrasse 26 | D-BSSE | WRO-1058 | CH-4058 Basel | Switzerland
http://c-cina.org | Tel. +41-61-387 32 62




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9, 41 -- 14.03.0279.002; Wed, 11 May 2016 13:30:30 +0200
9, 41 -- From: Henning Stahlberg {henning.stahlberg-at-unibas.ch}
9, 41 -- To: Microscopy {Microscopy-at-microscopy.com}
9, 41 -- CC: Ricardo Diogo Righetto {ricardo.righetto-at-unibas.ch} ,
9, 41 -- Robert McLeod
9, 41 -- {robert.mcleod-at-unibas.ch} ,
9, 41 -- Nikhil Biyani {nikhil.biyani-at-unibas.ch}
9, 41 -- Subject: Workshop announcement: Electron crystallography of 2D crystals of
9, 41 -- membrane proteins with 2DX, August 22-27, 2016
9, 41 -- Thread-Topic: Workshop announcement: Electron crystallography of 2D crystals
9, 41 -- of membrane proteins with 2DX, August 22-27, 2016
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From: microscopy.listserver-at-gmail.com
Date: May 11, 2016 at 3:49:29 AM CDT
Subject: [Microscopy] Workshop announcement: Electron crystallography of 2D crystals of

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues,

We are happy to announce the fifth 2DX Workshop on Electron Crystallography of Membrane Proteins, which will take place at the University of Basel (Switzerland), on August 22-27, 2016. The workshop will provide hands-on training on practical aspects of 2D crystal data collection on a Titan Krios, and cover in depth the theoretical foundations and the practical image processing of 2D crystal cryo-EM images with the 2DX software package. No previous experience is required.

Topics include:
Basics of 2D electron crystallography
* Introduction to 2D electron crystallography
* Cryo-electron microscopy sample preparation for 2D crystals of membrane proteins
* Cryo-EM data collection of 2D crystals
* Introduction to 2DX
* Drift correction using ZORRO
Basic image processing with 2DX
* Determination of the defocus, crystal tilt geometry, crystal lattice
* Crystal Unbending - including movie-mode drift correction of individual unit cells in dose-fractionated image series
* 2D merging and 3D merging with reconstruction via weighted back projection
* Automation of the processing pipeline
Advanced topics in image processing with 2DX
* Approaches for a partial reconstruction of amplitudes and phases inside of the missing cone
* Processing of 2D crystal unit cells with RELION and FREALIGN, under exploitation of neighborhood correlation of certain image parameters
* Advanced usage of the 2DX backend library
* Preparation of final results with quantitative description of statistical values (e.g., resolution) for export to databases (EMDB).

Computing infrastructure will be provided in place and participants are welcome to bring their own data sets and/or computer (OSX or Linux). More information can be found at
http://www.2dx.unibas.ch/workshop/2016

For registration, please include information about your affiliation, and a short motivation statement (max 200 words).
The registration fee is 250 CHF (travel and accommodation is not included), registration deadline is June 30th. As we can only provide a limited number of workstations, we typically accept participants on a "first come - first serve" basis. Please do not hesitate to contact us for any questions. Looking forward to welcoming you in Basel,

Nikhil Biyani,
Ricardo Righetto,
Robert McLeod,
Henning Stahlberg.

Henning Stahlberg, PhD
Prof. for Structural Biology, C-CINA, Biozentrum, University Basel
Mattenstrasse 26 | D-BSSE | WRO-1058 | CH-4058 Basel | Switzerland
http://c-cina.org | Tel. +41-61-387 32 62



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From: microscopy.listserver-at-gmail.com
Date: May 10, 2016 at 12:46:34 PM CDT
Subject: [Microscopy] Re: justification of industrial user fee in a university facility

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

There is a very good summary of many billing scenarios in the NIH FAQ issued in 2013. Well worth a read!

https://grants.nih.gov/grants/guide/notice-files/NOT-OD-13-053.html



Chris

Christopher J. Gilpin Ph.D.
Director, Life Science Microscopy Facility
Purdue University
Whistler Hall of Agriculture Research, Room S052
170 S. University St
West Lafayette, IN 47907
765-494-7750
gilpin-at-purdue.edu
lsmf-at-purdue.edu reaches everyone in the facility


-----Original Message-----
X-from: ajhall-at-prairienanotech.com [mailto:ajhall-at-prairienanotech.com]
Sent: Tuesday, May 10, 2016 1:11 PM
To: Gilpin, Christopher J {gilpin-at-purdue.edu}

Hi Microscopy Listerve and Shouliang Zhang,

This issue has been around for a long time. In general, university equipment is there to support the research efforts of their faculty and students. Commercial analytical labs exist which can serve industrial needs. University labs, though, are often tempted to sell their services to industry to make more money. A valid argument is often raised that this is unfair competition to those commercial labs because the (very
expensive) university equipment most likely has been purchased with federal grants to support academic research. Some feel this means universities should not do industrial work at all, and others feel it's OK so long as they don't undercut the commercial labs, which could open you up to a lawsuit. Some of the big commercial labs are VERY aggressive about this!

Note (for full disclosure): I am with a small commercial service lab but also work in an academic setting.

Allen J. Hall
Prairie Nanotechnology
www.prairienanotech.com

==============================Original Headers==============================
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4, 45 -- Date: Tue, 10 May 2016 11:50:44 -0500 4, 45 -- From: Allen Hall {ajhall-at-prairienanotech.com} 4, 45 -- User-Agent: Postbox 4.0.8 (Macintosh/20151105) 4, 45 -- MIME-Version: 1.0 4, 45 -- To: Microscopy-at-microscopy.com 4, 45 -- Subject: Re: justification of industrial user fee in a university facility 4, 45 -- Content-Type: text/plain; charset=windows-1252 4, 45 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: May 10, 2016 at 11:50:42 AM CDT
Subject: [Microscopy] Re: justification of industrial user fee in a university facility

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Microscopy Listerve and Shouliang Zhang,

This issue has been around for a long time. In general, university
equipment is there to support the research efforts of their faculty and
students. Commercial analytical labs exist which can serve industrial
needs. University labs, though, are often tempted to sell their services
to industry to make more money. A valid argument is often raised that
this is unfair competition to those commercial labs because the (very
expensive) university equipment most likely has been purchased with
federal grants to support academic research. Some feel this means
universities should not do industrial work at all, and others feel its
OK so long as they dont undercut the commercial labs, which could open
you up to a lawsuit. Some of the big commercial labs are VERY aggressive
about this!

Note (for full disclosure): I am with a small commercial service lab but
also work in an academic setting.

Allen J. Hall
Prairie Nanotechnology
www.prairienanotech.com

==============================Original Headers==============================
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From: microscopy.listserver-at-gmail.com
Date: May 10, 2016 at 9:45:43 AM CDT
Subject: [Microscopy] Re: Cryomicrotomy using glass knives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,

Many thanks for all your (so many) answers! The outcome is unambigous
-- nail polish. Now up to me to test.

Kind regards,
Ondrej



On 4 May 2016 1:01 pm, "Ondej Koteck" {ondrej.kotecky-at-gmail.com} wrote:

Dear Listers,

Since years we use diamond knives to cut ultrathin sections of
polymers at low temperature (around -150 *C). When cut the sections
are floating boat/trough integrated on the diamond knife, from where
they are collected.

To cut larger sections we started to make and use glass knives on
which a plastic boat/trough is glued using a "Cavex Set Up Wax", a
dentistry modelling wax, as suggested by the vendor. Unfortunately the
boats fall off at low temperature.

Can anyone suggest a solution or a "glue" that would resist to low temperatures?

Many thanks,
Ondrej


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From: microscopy.listserver-at-gmail.com
Date: May 10, 2016 at 6:32:52 AM CDT
Subject: [Microscopy] viaWWW: JEOL 2010F Available

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Title-Subject: [Filtered] JEOL 2010F

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From: microscopy.listserver-at-gmail.com
Date: May 10, 2016 at 6:31:23 AM CDT
Subject: [Microscopy] viaWWW:Used 3mm TEM disc puncher, and Tenupol-5 jet

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Email: fei.long-at-queensu.ca Name: Fei Long

Organization: Queen's University

Title-Subject: [Filtered] Used 3mm TEM disc puncher, and Tenupol-5 jet

Message: Hi all,
I am looking to buy a used TEM 3mm disc puncher. Also a set of the jets with 1mm bore for Tenupol-5
electro-polisher, since the ones I have is broken now.

If anyone has these spare or none used parts, I am interested to purchase them.
Thanks,

Fei

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From: microscopy.listserver-at-gmail.com
Date: May 9, 2016 at 4:11:00 PM CDT
Subject: [Microscopy] 35 mm camera and controller for Zeiss Axioplan to give away

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Colleagues,
For any film aficianados out there, I have a
cameraback and mounting bits, as well as the electronic controller, for
a 35 mm camera, that coupled to a Zeiss Axioplan. I was using it up
until around 2002 or so, when it was working fine. It has been kept cool
and out of dust since then. I think it could probably be adapted to any
brand of scope with a bit of hardware tinkering. Free to anyone who
wants it (I am willing to pack it carefully for shipment but not pay
shipping costs). Thanks,
Tobias
University of Massachusetts, Biology Department, 611 N Pleasant St,
Amherst, MA, 01003, USA

==============================Original Headers==============================
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From: microscopy.listserver-at-gmail.com
Date: May 9, 2016 at 1:23:05 PM CDT
Subject: [Microscopy] viaWWW:TEM: Unknown structures after HPF and AFS in

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Hi Elizabeth

I agree with Michael's comments, these holes are typical for uninfiltrated
dense material. Try letting your specimens sit in uncatalyzed resin at room
temperature a bit longer. For Epon I usually do 3h 25%, over night 50% and
2x2h 100% before going into catalyzed resin at 60C 24h. Leaving out the
catalyst will also improve the viscosity. You can even heat up the
uncatalyzed resin to 37C if you want to push it.
You do have quite some crystal damage from freezing, that together with a
dry acetone FS will extract a considerable amount of material (see my follow
up paper to Walther & Ziegler for more details,
http://www.ncbi.nlm.nih.gov/pubmed/18445157 ). Poorly frozen material
extracts a lot easier. Here are a few suggestions:

1. If you have adherent cells on sapphire discs, lift them out of the
culture dish, touch a filter paper to remove excess medium, dip in
hexedecene, then mount for HPF. This ensures you're only freezing the
minimal layer of medium with your cells. Freezing adherent cells in pure
medium and 200 um deep carriers wil almost certainly fail without
cryoprotection as you're essentially tryin to freeze 200 um of pure water.
(safety tip: repeated skin exposure to hexadecene will give you contact
dermatitis, wear nitrile gloves).
2. If you're working with suspensions, 100 um deep carriers and 20% BSA for
cryoprotection works well with most cells and doesn't mess up your FS (in
contrast to sugars that won't come out easily during FS).
3. FS in acetone, about 1% osmium tetroxide, about 0.1% uranyl acetate,
about 5% water for best contrast. To get there, dissolve OsO4 in acetone,
add 1:20 of your 2% aqueous UA and that's it. Some precipitation is normal
and doesn't influence the end result, it's a saturated solution particularly
at cold temperatures. If it's completely snowed up, try half the amount of
2% UA.
4. I'd advise against prefixation in aldehydes unless your cells are very
sensitive (e.g. primary cells) and you can't get them to the HPF within
30ish minutes.

Good luck!
Chris


-----Original Message-----
X-from: mdelann1-at-jhmi.edu [mailto:mdelann1-at-jhmi.edu]
Sent: Monday, May 9, 2016 10:24 AM
To: cbuser125-at-gmail.com

Hello Elizabeth and Welcome to the Forum, These "holes" look like
un-infiltrated granules. I would see this sometimes with Drosophila eyes
and the pigment granules. Some would be infiltrated and others not. The
fix was to do longer and more infiltration steps
(resin:solvent) and/or switch to a less viscous resin. Were these cells
grown on a sapphire disc or is this a suspension that was HPF/AFS? I know
mast cells have a large number of secretory granules, but not outside the
cells plasma memebrane. Also what is you AFS cocktail? I would also
introduce 5% water to your freezing cocktail for better membrane
preservation, (Walther, P. & Ziegler, A. Freeze substitution of
high-pressure frozen samples: the visibility of biological membranes is
improved when the substitution medium contains water. J. Microsc. 208,
3-10). Also what was your cryo-protectant? (what did you HPF the cells in).
I believe the HPF images should look a lot better, it looks like there is
some freezing artifacts in your cells (look for spider-web like profiles)
and your mitochondria are extracted. Were these cells frozen live? If they
are clinical you can use the HPF-chemical hybrid technique where you lightly
fix the sample in paraformaldehyde only until you can get to the HPF. If
you need a good reference for the HPF/AFS techniques, Mary Morphew at
Colorado (formerly Kent McDonald's lab) has a very detailed manual online:
https://mcdb.colorado.edu/facilities/ems/pdf/mmanual.pdf. Finally all HPF
experiments should be accompanied by the standard fixation counterpart for
comparison. Perhaps this will give you a clue. Please keep us posted as to
you results.

Sincerely,
Michael Delannoy

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Email: elisabeth.pritz-at-medunigraz.at Name: Elisabeth Pritz

Organization: Medical Univeristiy Graz

Title-Subject: [Filtered] TEM: Unknown structures after HPF and AFS in RBL
cell line

Message: Hello,

it's my first time using this forum - maybe someone knows these structures
and it would be a great help for me to learn more about ultrastructure of
biological specimen.
We got some unknown structures after high pressure freezing and automated
freeze substitution of mast cell line RBL-2H3 (it is not fully
representative for mast cell line).

There is a ring of "bubbles" around each cell. However, it seems that the
bubbles were filled (please use link below for downloading the pictures).
As mentioned before we did high pressure freezing and freeze substitution in
a mixture of acetone
(waterfree) with osmium tetroxide and uranylacetate. The samples were
embedded in TAAB epoxy resin.
Unfortunately the membrane contrast is very poor in these samples, though we
already fixed that problem.
These "bubbles" appear only close to the cells and in nearly every single
bubble one could see electron dense remnants.

Is it possible, that the structures are a kind of degranulated vesicles?

Thanks for your support!

BR
Elisabeth



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From: microscopy.listserver-at-gmail.com
Date: May 9, 2016 at 12:47:47 PM CDT
Subject: [Microscopy] viaWWW:TEM: Unknown structures after HPF and AFS in

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi
I have very limited experience in TEM so please don't laugh: could they be
air associated or originated from the cell, that formed bubbles during the
procedure?
yorgos

Dr Yorgos Nikas
Athens Innovative Microscopy
Skra 36 Voula 16673 GREECE

Tel/fax +30 210 8957677
mobile +30 6945 107477
www.eikonika.net www.aim.cat
*************************************




-----Original Message-----
X-from: mdelann1-at-jhmi.edu [mailto:mdelann1-at-jhmi.edu]
Sent: Monday, May 9, 2016 8:12 PM
To: eikonika-at-otenet.gr

Hello Elizabeth and Welcome to the Forum, These "holes" look like
un-infiltrated granules. I would see this sometimes with Drosophila eyes
and the pigment granules. Some would be infiltrated and others not. The
fix was to do longer and more infiltration steps
(resin:solvent) and/or switch to a less viscous resin. Were these cells
grown on a sapphire disc or is this a suspension that was HPF/AFS? I know
mast cells have a large number of secretory granules, but not outside the
cells plasma memebrane. Also what is you AFS cocktail? I would also
introduce 5% water to your freezing cocktail for better membrane
preservation, (Walther, P. & Ziegler, A. Freeze substitution of
high-pressure frozen samples: the visibility of biological membranes is
improved when the substitution medium contains water. J. Microsc. 208,
3-10). Also what was your cryo-protectant? (what did you HPF the cells in).
I believe the HPF images should look a lot better, it looks like there is
some freezing artifacts in your cells (look for spider-web like profiles)
and your mitochondria are extracted. Were these cells frozen live? If they
are clinical you can use the HPF-chemical hybrid technique where you lightly
fix the sample in paraformaldehyde only until you can get to the HPF. If
you need a good reference for the HPF/AFS techniques, Mary Morphew at
Colorado (formerly Kent McDonald's lab) has a very detailed manual online:
https://mcdb.colorado.edu/facilities/ems/pdf/mmanual.pdf. Finally all HPF
experiments should be accompanied by the standard fixation counterpart for
comparison. Perhaps this will give you a clue. Please keep us posted as to
you results.

Sincerely,
Michael Delannoy

-----Original Message-----
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[mailto:microscopy.listserver-at-gmail.com]
Sent: Monday, May 09, 2016 9:05 AM
To: mdelann1-at-jhmi.edu

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Email: elisabeth.pritz-at-medunigraz.at Name: Elisabeth Pritz

Organization: Medical Univeristiy Graz

Title-Subject: [Filtered] TEM: Unknown structures after HPF and AFS in RBL
cell line

Message: Hello,

it's my first time using this forum - maybe someone knows these structures
and it would be a great help for me to learn more about ultrastructure of
biological specimen.
We got some unknown structures after high pressure freezing and automated
freeze substitution of mast cell line RBL-2H3 (it is not fully
representative for mast cell line).

There is a ring of "bubbles" around each cell. However, it seems that the
bubbles were filled (please use link below for downloading the pictures).
As mentioned before we did high pressure freezing and freeze substitution in
a mixture of acetone
(waterfree) with osmium tetroxide and uranylacetate. The samples were
embedded in TAAB epoxy resin.
Unfortunately the membrane contrast is very poor in these samples, though we
already fixed that problem.
These "bubbles" appear only close to the cells and in nearly every single
bubble one could see electron dense remnants.

Is it possible, that the structures are a kind of degranulated vesicles?

Thanks for your support!

BR
Elisabeth



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and AFS in RBL cell line
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From: microscopy.listserver-at-gmail.com
Date: May 9, 2016 at 11:59:31 AM CDT
Subject: [Microscopy] viaWWW:TEM: Unknown structures after HPF and AFS in

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Elizabeth and Welcome to the Forum,
These "holes" look like un-infiltrated granules. I would see this sometimes
with Drosophila eyes and the pigment granules. Some would be infiltrated
and others not. The fix was to do longer and more infiltration steps
(resin:solvent) and/or switch to a less viscous resin. Were these cells
grown on a sapphire disc or is this a suspension that was HPF/AFS? I know
mast cells have a large number of secretory granules, but not outside the
cells plasma memebrane. Also what is you AFS cocktail? I would also
introduce 5% water to your freezing cocktail for better membrane
preservation, (Walther, P. & Ziegler, A. Freeze substitution of
high-pressure frozen samples: the visibility of biological membranes is
improved when the substitution medium contains water. J. Microsc. 208,
3-10). Also what was your cryo-protectant? (what did you HPF the cells in).
I believe the HPF images should look a lot better, it looks like there is
some freezing artifacts in your cells (look for spider-web like profiles)
and your mitochondria are extracted. Were these cells frozen live? If they
are clinical you can use the HPF-chemical hybrid technique where you lightly
fix the sample in paraformaldehyde only until you can get to the HPF. If
you need a good reference for the HPF/AFS techniques, Mary Morphew at
Colorado (formerly Kent McDonald's lab) has a very detailed manual online:
https://mcdb.colorado.edu/facilities/ems/pdf/mmanual.pdf. Finally all HPF
experiments should be accompanied by the standard fixation counterpart for
comparison. Perhaps this will give you a clue. Please keep us posted as to
you results.

Sincerely,
Michael Delannoy

-----Original Message-----
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Sent: Monday, May 09, 2016 9:05 AM
To: mdelann1-at-jhmi.edu

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Email: elisabeth.pritz-at-medunigraz.at Name: Elisabeth Pritz

Organization: Medical Univeristiy Graz

Title-Subject: [Filtered] TEM: Unknown structures after HPF and AFS in RBL
cell line

Message: Hello,

it's my first time using this forum - maybe someone knows these structures
and it would be a great help for me to learn more about ultrastructure of
biological specimen.
We got some unknown structures after high pressure freezing and automated
freeze substitution of mast cell line RBL-2H3 (it is not fully
representative for mast cell line).

There is a ring of "bubbles" around each cell. However, it seems that the
bubbles were filled (please use link below for downloading the pictures).
As mentioned before we did high pressure freezing and freeze substitution in
a mixture of acetone
(waterfree) with osmium tetroxide and uranylacetate. The samples were
embedded in TAAB epoxy resin.
Unfortunately the membrane contrast is very poor in these samples, though we
already fixed that problem.
These "bubbles" appear only close to the cells and in nearly every single
bubble one could see electron dense remnants.

Is it possible, that the structures are a kind of degranulated vesicles?

Thanks for your support!

BR
Elisabeth



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From: microscopy.listserver-at-gmail.com
Date: May 9, 2016 at 8:32:01 AM CDT
Subject: [Microscopy] New photo printer for micrographs, for those interested

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

To recap: I placed a request for opinions on photo printers for
micrographs, now that HP seems to be out of the game.
I got a few responses (surprisingly few, given past interest in this
topic), but those that expressed a preference all liked the Canon P600.
Which does look good on paper (it's Monday morning, so that's the best
pun I've got).

Thanks to all who responded.

Phil
--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

==============================Original Headers==============================
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From: microscopy.listserver-at-gmail.com
Date: May 9, 2016 at 7:43:53 AM CDT
Subject: [Microscopy] viaWWW:TEM: Unknown structures after HPF and AFS in RBL cell line

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Email: elisabeth.pritz-at-medunigraz.at Name: Elisabeth Pritz

Organization: Medical Univeristiy Graz

Title-Subject: [Filtered] TEM: Unknown structures after HPF and AFS in RBL cell line

Message: Hello,

it's my first time using this forum - maybe someone knows these structures and it would be a great
help for me to learn more about ultrastructure of biological specimen.
We got some unknown structures after high pressure freezing and automated freeze substitution of
mast cell line RBL-2H3 (it is not fully representative for mast cell line).

There is a ring of "bubbles" around each cell. However, it seems that the bubbles were filled
(please use link below for downloading the pictures).
As mentioned before we did high pressure freezing and freeze substitution in a mixture of acetone
(waterfree) with osmium tetroxide and uranylacetate. The samples were embedded in TAAB epoxy resin.
Unfortunately the membrane contrast is very poor in these samples, though we already fixed that problem.
These "bubbles" appear only close to the cells and in nearly every single bubble one could see
electron dense remnants.

Is it possible, that the structures are a kind of degranulated vesicles?

Thanks for your support!

BR
Elisabeth



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From: microscopy.listserver-at-gmail.com
Date: May 8, 2016 at 2:19:42 PM CDT
Subject: [Microscopy] viaWWW:detailed instructions for LEO (Zeiss) 1450VP SEM

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Email: scottserata-at-gmail.com Name: Scott Serata

Organization: California Academy of Sciences/JEOL

Title-Subject: [Filtered] detailed instructions for LEO (Zeiss) 1450VP SEM

Message: Several people have asked me to post this on the listserve. I have written detailed
instructions for the LEO/Zeiss 1450VP SEM. I was the engineer at the California Academy of Sciences
responsible for training users and fixing this machine for 15 years, until I finally got rid of it
last year. (I now work for JEOL) Our machine did not have any analytical equipment (X-ray EDS/WDS)
on it, just the SE, VPSE and BSD detectors. If you have a LEO 1450 VP SEM this maybe useful. It is
in PDF format and about 13 MB.

Scott Serata

click on this link to get it from my Google drive
https://drive.google.com/open?id=0B2-Pe5FzqYv2TWdUNXNLQTZLQmM

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From: microscopy.listserver-at-gmail.com
Date: May 8, 2016 at 8:40:51 AM CDT
Subject: [Microscopy] viaWWW:Free Megaview II CCD System for TEM

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Email: Hobie-at-technicalsalessolutions.com
Name: Hobie Richards

Organization: Technical Sales Solutions

Title-Subject: [Filtered] Free Megaview II CCD System for TEM

Message: TSS has one complete Megaview II CCD Side-mount Camera for Philips/FEI Series TEMs that
needs to find a home. Functional and complete side-mount CCD camera solution with computer and
cable set.
Pictures may be seen of the camera/flange here:

https://tss.exavault.com/share/view/bemn-fgdqg1k7

Will carefully prepare for shipment and with your shipping label and send to you anywhere in the world.


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From: microscopy.listserver-at-gmail.com
Date: May 6, 2016 at 9:23:11 AM CDT
Subject: [Microscopy] RE: viaWWW: justification of industrial user fee in a

Contents Retrieved from Microscopy Listserver Archives
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Like you say, we take a look at local rates for similar services and price = higher so as to not undercut the local business.

If anyone gives me a hard time about rates I compare it to what they would = pay a professional photographer to take high school senior pictures or wedd= ing photos. EM Lab rates start to look entirely reasonable, especially give= n our "camera" is on the order of hundreds of thousands of dollars instead = of hundreds of dollars.

Kind regards,

William Stonewall Monroe
University of Alabama -at- Birmingham SEM Lab
http://www.uab.edu/scanningelectronmicroscopy


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From: microscopy.listserver-at-gmail.com
Date: May 5, 2016 at 8:16:26 PM CDT
Subject: [Microscopy] viaWWW:justification of industrial user fee in a uiversity facility

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Email: shouliang.zhang-at-gmail.com Name: Shouliang Zhang

Title-Subject: [Filtered] justification of industrial user fee in a uiversity facility
Message: Dear listers,
How do your facilities, especially for the electron microscopes, justify the industrial user rate?
Do you consider the local analytical companies rate so that yours won't undercut theirs? I looked up
the SEM/TEM rate across the universties nationwide and found it varies a lot, ranging from less
$100/h to $300/h for industrial users. Thanks.

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From: microscopy.listserver-at-gmail.com
Date: May 5, 2016 at 1:47:38 PM CDT
Subject: [Microscopy] Electrical feedthroughs

Contents Retrieved from Microscopy Listserver Archives
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If you are not fussy or fancy just drive some sharpened 1/8" diameter
stainless steel rods through a clean rubber stopper. Will work OK
through the HV range (10^-6 Torr) , but probably not into the UHV range.

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From: microscopy.listserver-at-gmail.com
Date: May 5, 2016 at 12:42:03 PM CDT
Subject: [Microscopy] Re: viaWWW:electrical pass through for FEI ESEM

Contents Retrieved from Microscopy Listserver Archives
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My friend recently made a feedthrough using stiff piano wire, a rubber
stopper from home depot, and super glue (I think, or maybe it was
torr-seal type epoxy). He used a DB9 port to mark the pin locations,
then drilled pilot holes (which I don't think were fully drilled
through the rubber) and I believe then he pounded the wire through the
remainder of the rubber. This stopper was jammed into a tapered hole
in a blank/unused blocking plate that came with his machine. It holds
working pressure as good as before the feedthrough was installed. If
you want more info, I can probably pass you his direct email to ask
for more details.

On Thu, May 5, 2016 at 5:39 AM, {microscopy.listserver-at-gmail.com} wrote:



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Email: wambrose-at-unc.edu Name: Wallace Ambrose

Organization: CHANL UNC

Title-Subject: [Filtered] electrical pass through for FEI ESEM
Message: Hello, I would like to do voltage contrast and active EBIC on our FEI Quanta ESEM and
Helios FIB. I am interested in knowing what folks have done to make up pass through plates (and what
connectors (BNC, DB9 or DB25) would be the most useful for this for the 4 or 2 3/8 inch ports.
thanks
Wallace
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From: microscopy.listserver-at-gmail.com
Date: May 5, 2016 at 6:43:39 AM CDT
Subject: [Microscopy] viaWWW:Cryomicrotomy using glass knives

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Email: duraine-at-bcm.edu Name: Lita Duraine

Organization: Howard Hughes Medical Institute

Title-Subject: [Microscopy] RE: Cryomicrotomy using glass knives

Message: Hi Ondrej,

At Delta microscopy school some of us used 3M Silver Polyester Tape, Nonconductive, 850 from Ted
Pella to make boats for glass knives. I think the tape is rated for -150 degrees C. Ted Pella also
carries other type tapes that may be used for making boats with lower temp ranges. You might check.

Regards,

Lita Duraine
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From: microscopy.listserver-at-gmail.com
Date: May 5, 2016 at 6:42:44 AM CDT
Subject: [Microscopy] viaWWW:Importing .txt files into Digital Micrograph for EELS

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Email: peter.eschbach-at-oregonstate.edu Name: Peter Eschbach

Organization: Oregon State University

Title-Subject: [Filtered] Importing .txt files into Digital Micrograph for EELS

Message: Hi:

We do not have a digiscan box to control our Titan from Digital Micrograph (DM). So, for now we are
collecting simultaneous EELS and EDX linescans in FEI's TIA software. I can do rudimentary analysis
in TIA on all the EELS data but would like to import several EELS spectra from my linescan into DM.
I am able to save the TIA acquired EELS data into .txt files with two nice columns, Energy and
Intensity. But, does anybody know of a script to convert the .txt files into DM3? We looked on
David Mitchell's site, but nothing jumped out.
Thanks,
Pete Eschbach
Oregon State University EM Facility
541 737 5645

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From: microscopy.listserver-at-gmail.com
Date: May 5, 2016 at 6:40:33 AM CDT
Subject: [Microscopy] viaWWW:electrical pass through for FEI ESEM

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Email: wambrose-at-unc.edu Name: Wallace Ambrose

Organization: CHANL UNC

Title-Subject: [Filtered] electrical pass through for FEI ESEM
Message: Hello, I would like to do voltage contrast and active EBIC on our FEI Quanta ESEM and
Helios FIB. I am interested in knowing what folks have done to make up pass through plates (and what
connectors (BNC, DB9 or DB25) would be the most useful for this for the 4 or 2 3/8 inch ports.
thanks
Wallace
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From: microscopy.listserver-at-gmail.com
Date: May 4, 2016 at 7:24:33 PM CDT
Subject: [Microscopy] Pump speed for wafer holder

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Don:
A pumping speed of 1 cfm is a pretty high rate of flow (28 liters/min)
and typical of values for small rotary vane pumps. For this type of pump
the speed is usually rated as the volume of gas moved through the pump
when both the inlet and outlet ports are at atmospheric presure. I
wonder if you would really need a pumping speed of 1 cfm for a device
that merely holds a wafer in place. The question is, what is the actual
volume that needs to be evacuated, and how much of a hurry are you in to
get the wafer held firmly in place. If you just need to evacuate a tube
of modest diameter and modest length leading to a small chamber under
the wafer you certainly don't need a speed of 1 cfm to do the job, even
if the system leaks pretty badly.

Wil

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From: microscopy.listserver-at-gmail.com
Date: May 4, 2016 at 3:13:57 PM CDT
Subject: [Microscopy] Re: how are vacuum pumps rated?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Fred,
Thanks very much for this explanation.
Don

----- Original Message -----
From: Frederick Schamber
To: Don Chernoff at ASM
Sent: Wednesday, May 04, 2016 5:31 PM
Subject: [a] Re: how are vacuum pumps rated?


Three things: (1) Vacuum pump ratings (pumping speed) always apply to the
inlet (evacuated).

(2) If they say cfm (or lps) it is 'pumping speed' and that is independent
of either internal or external pressure. Think of having a 'demon'
collecting one cubic foot of gas in a (previously evacuated) canister while
inside the chamber. The demon then seals the canister and removes it from
the chamber. To repeat the cycle he shrinks the canister to zero volume (
thereby expelling the collected gas) and then goes back into the chamber
where he expands it to 1 cubic foot again. If he does this once a minute,
that's a 1 cfm pumping speed. It's easiest to see that's what a piston pump
does but it's the same principle for rotary vane (and can be generalized for
a turbo). If you think of the 'demon' model it's clear that the volume per
second is a function only of the canister size and repetition rate, not the
pressure (either internal nor external. That's an idealization for an
actual pump of course due to things like leakage, turbulent flow, and lots
of other messy things. But in principle, volume pumping speed is constant.

(3) Mass flow rate is density times pumping speed and would be something
like grams per second. Since the density of the gas decreases, the mass
flow rate also decreases with improving vacuum. A pump's 'throughout' is
defined as inlet pressure x pumping speed and is proportional to mass flow
rate.

Actually, mass flow rate (and throughout) can be defined and is invariant
anywhere in the system, so long as volume and density are defined at the
same place. So you can use the constancy of throughput to calculate the
volume of gas exhausted for a given pumping speed and inlet pressure.

Any help?

Fred

Sent from my iPhone

On May 4, 2016, at 4:14 PM, Don Chernoff at ASM {donc-at-asmicro.com}
wrote:

Dear Fred,
Thanks for your reply. I you're pointing in the right direction. My
question is a practical one: if someone says "you need a flow rate of 1
cfm"
does that mean 1 cfm measured at P = 1 atmosphere?
Don
----- Original Message ----- From: Frederick Schamber
To: donc-at-asmicro.com
Sent: Wednesday, May 04, 2016 3:13 PM

Dear Fred,
Thanks for your reply. I you're pointing in the right direction. My
question is a practical one: if someone says "you need a flow rate of 1 cfm"
does that mean 1 cfm measured at P = 1 atmosphere?
Don
----- Original Message -----
From: Frederick Schamber
To: donc-at-asmicro.com
Sent: Wednesday, May 04, 2016 3:13 PM
Subject: [a] Re: [Microscopy] how are vacuum pumps rated?


Don,


Maybe I misunderstand your question, but I was long puzzled by how
mechanical vacuum pumps are rated. Finally I realized that the mechanism of
a vacuum pump will capture and remove a constant volume of gas regardless of
internal pressure. That is, regardless of that internal pressure, the
volume swept out remains the same. Clearly, that means that the volume of
gas exhausted decreases proportionately as the internal pressure declines.
This is why we use 'mass flow' when we want to quantify the mass of material
pumped.


So if you are looking at volume of gas removed from the chamber the
pumping speed is (essentially) constant. But if you are looking at volume
or mass of gas exhausted, the pumping speed varies proportionately to
internal pressure.


Does this help?


Fred Schamber (retired, but still interested)


On Wed, May 4, 2016 at 2:27 PM, {donc-at-asmicro.com} wrote:




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Dear All,
I want to thank the many people who responded to my initial post about
low
vacuum pump.

In reviewing vacuum pump specifications, I note that the flow rate chart
shows a high rate at atmospheric pressure with a rapid decrease in rate
as
the input pressure decreases. When this type of pump is specified, is
there
general agreement that the flow rate is specified for free air (at 1
atmosphere pressure)?
regards,
Don
=============================================
Don Chernoff, Ph.D., President
Advanced Surface Microscopy, Inc.; 3250 N. Post Rd., Suite 120;
Indianapolis IN 46226 USA
E-Mail: donc-at-asmicro.com www.asmicro.com
Voice: +1-317-895-5630 Toll free: +1-800-374-8557 (in USA & Canada)
Fax: +1-317-895-5652
[business activities since 1990: analytical services in AFM, AFM
probes,
consulting, training, calibration and test specimens,
calibration and measurement software, used NanoScope equipment.]
=============================================
----- Original Message -----
From: Don Chernoff at ASM
To: Microscopy List
Sent: Sunday, May 01, 2016 7:01 PM
Subject: low vacuum pump


I'm working with a wafer inspection system that uses vacuum to clamp
the
wafer. The manufacturer's specifications call for a modest vacuum:

low vacuum: 150 torr (-24" Hg)

flow rate: { 1 cfm (28 l/m)

Actual flow will be intermittent.



What type of pump would be suitable for this application?

I welcome suggestions for specific models and suggestions for how to
configure the pump.

regards,
Don
=============================================
Don Chernoff, Ph.D., President
Advanced Surface Microscopy, Inc.; 3250 N. Post Rd., Suite 120;
Indianapolis IN 46226 USA
E-Mail: donc-at-asmicro.com www.asmicro.com
Voice: +1-317-895-5630 Toll free: +1-800-374-8557 (in USA & Canada)
Fax: +1-317-895-5652
[business activities since 1990: analytical services in AFM, AFM
probes,
consulting, training, calibration and test specimens,
calibration and measurement software, used NanoScope equipment.]
=============================================


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From: microscopy.listserver-at-gmail.com
Date: May 4, 2016 at 12:57:53 PM CDT
Subject: [Microscopy] how are vacuum pumps rated?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,
I want to thank the many people who responded to my initial post about low
vacuum pump.

In reviewing vacuum pump specifications, I note that the flow rate chart
shows a high rate at atmospheric pressure with a rapid decrease in rate as
the input pressure decreases. When this type of pump is specified, is there
general agreement that the flow rate is specified for free air (at 1
atmosphere pressure)?
regards,
Don
=============================================
Don Chernoff, Ph.D., President
Advanced Surface Microscopy, Inc.; 3250 N. Post Rd., Suite 120;
Indianapolis IN 46226 USA
E-Mail: donc-at-asmicro.com www.asmicro.com
Voice: +1-317-895-5630 Toll free: +1-800-374-8557 (in USA & Canada)
Fax: +1-317-895-5652
[business activities since 1990: analytical services in AFM, AFM probes,
consulting, training, calibration and test specimens,
calibration and measurement software, used NanoScope equipment.]
=============================================
----- Original Message -----
From: Don Chernoff at ASM
To: Microscopy List
Sent: Sunday, May 01, 2016 7:01 PM
Subject: low vacuum pump


I'm working with a wafer inspection system that uses vacuum to clamp the
wafer. The manufacturer's specifications call for a modest vacuum:

low vacuum: 150 torr (-24" Hg)

flow rate: { 1 cfm (28 l/m)

Actual flow will be intermittent.



What type of pump would be suitable for this application?

I welcome suggestions for specific models and suggestions for how to
configure the pump.

regards,
Don
=============================================
Don Chernoff, Ph.D., President
Advanced Surface Microscopy, Inc.; 3250 N. Post Rd., Suite 120;
Indianapolis IN 46226 USA
E-Mail: donc-at-asmicro.com www.asmicro.com
Voice: +1-317-895-5630 Toll free: +1-800-374-8557 (in USA & Canada)
Fax: +1-317-895-5652
[business activities since 1990: analytical services in AFM, AFM probes,
consulting, training, calibration and test specimens,
calibration and measurement software, used NanoScope equipment.]
=============================================


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From: microscopy.listserver-at-gmail.com
Date: May 4, 2016 at 5:59:01 AM CDT
Subject: [Microscopy] Cryomicrotomy using glass knives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,

Since years we use diamond knives to cut ultrathin sections of
polymers at low temperature (around -150 *C). When cut the sections
are floating boat/trough integrated on the diamond knife, from where
they are collected.

To cut larger sections we started to make and use glass knives on
which a plastic boat/trough is glued using a "Cavex Set Up Wax", a
dentistry modelling wax, as suggested by the vendor. Unfortunately the
boats fall off at low temperature.

Can anyone suggest a solution or a "glue" that would resist to low temperatures?

Many thanks,
Ondrej


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From: microscopy.listserver-at-gmail.com
Date: May 3, 2016 at 2:10:39 PM CDT
Subject: [Microscopy] Lab Technician Job at the America Museum of Natural History

Contents Retrieved from Microscopy Listserver Archives
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The Laboratory Technician will be responsible for assisting and/or training scientific staff and students with Scanning Electron Microscopy, X-ray microanalysis, Laser Scanning Confocal Microscopy, Cathodoluminescence Spectroscopy, X-ray Computed Tomography, specimen preparation and image processing and printing; will maintain functionality and capability of lab equipment; will undergo periodic training on new technologies; will order supplies and track usage and expenditures; will interact with curatorial staff and students on diverse projects using microscopy and digital imaging in the fields of zoology, paleontology, anthropology, archeology, and earth and planetary sciences.

SEM (EDS+EBSD+CL)
Micro-computed tomography
Laser scanning confocal microscopy + Raman spectroscopy
TEM

careers.amnh.org/applicants/Central?quickFind=52033

Henry Towbin
Laboratory Co-manager
Microscopy and Imaging Facility
American Museum of Natural History
79th St & Central Park West
NY, NY 10024



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From: microscopy.listserver-at-gmail.com
Date: May 3, 2016 at 9:37:21 AM CDT
Subject: [Microscopy] [Microscopy}Correction, Low Vac pump

Contents Retrieved from Microscopy Listserver Archives
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Don:
I made a typo in my previous message. The correct model number for the
KNF Mini Diaphragm Pump is N 84.3 ANI.

If you decide to use this pump you will need a base plate to mount it on
and a housing to shield the cooling fan. I can supply drawings for
versions of these items that I'v used previously that are easy and cheap
to make.

You will probably also want some vacuum valves, and for this purpose I
can recommend the mini ball valves (such as Model 4114T11, T13. T64)
sold by McMaster Carr (mcmastercarr.com, 562-692-5911). They also sell a
full line of tubing fittings for polyethylene tubing.

Wil Bigelow

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From: microscopy.listserver-at-gmail.com
Date: May 2, 2016 at 9:22:16 PM CDT
Subject: [Microscopy] Low Vacuum Pump

Contents Retrieved from Microscopy Listserver Archives
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I think perhaps the Model N 64.3 ANI Mini Diaphragm Pump manufactured
by KNF Neuberger (www.knf.com/usa.htm) would fill the bill. It will
produce a vacuum below 10 Torr, has a speed of about 4 l//min and costs
less than $600. I've used one of these on a couple of systems I've
designed in the past with very satisfactory results.
Wil Bigelow

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From: microscopy.listserver-at-gmail.com
Date: May 2, 2016 at 2:31:46 AM CDT
Subject: [Microscopy] Re: Photo printers

Contents Retrieved from Microscopy Listserver Archives
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Listers,

I haven't had to buy a photo printer for micrographs for some years.
What's the current best? HP doesn't make the Photosmart series anymore,
and the Epson Sure color series specs look good, but ... ?
I need both color and greyscale.
Maybe the Epson P600?
Thanks.

Hi Phil, and colleagues on the list,
we have bought an Officejet Pro 8100 two weeks ago, and we have an Officejet Pro 8000 in use since a few years, directly at a TEM with 2k x 2k camera - to the delight of the users.
no interest in the manufacturing company, of course ...
kind regards,
Reinhard

--
Prof. Dr. Reinhard Rachel
University of Regensburg
Centre for EM / Anatomy
Faculty of Biology & Preclin. Med.
Universitaetsstrasse 31
D-93053 Regensburg - Germany
tel +49 941 943 -2837, -1720
mail reinhard.rachel-at-biologie.uni-regensburg.de
office: VKL 3.1.29

Next microscopy conferences:
- 16th Europ Microsc Congress EMC
http://www.emc2016.fr/en/
28 Aug - 2 Sept 2016 in Lyon, FR
- Microscopy Conference 2017
Dreilaendertagung Lausanne, CH
20-25 August 2017
- next Microbiol. conferences:
VAAM/DGHM - Annual Conf
March 5-8, 2017, Wuerzburg




==============================Original Headers==============================
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From: microscopy.listserver-at-gmail.com
Date: May 1, 2016 at 6:05:53 PM CDT
Subject: [Microscopy] Clean dry air

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I'm working with a wafer inspection system that uses clean dry air to float
a vibration isolation table and to release parts from vacuum clamps.

The manufacturer's specifications call for:

Pressure: 80-100 psi

Flow rate: { 1 cfm (28 l/m)

Cleanliness: Filtered to Class I



The actual flow will be intermittent.

I'm looking a quiet system that is easy to maintain. I would like to
receive suggestions of specific equipment configurations.



regards,
Don
=============================================
Don Chernoff, Ph.D., President
Advanced Surface Microscopy, Inc.; 3250 N. Post Rd., Suite 120;
Indianapolis IN 46226 USA
E-Mail: donc-at-asmicro.com www.asmicro.com
Voice: +1-317-895-5630 Toll free: +1-800-374-8557 (in USA & Canada)
Fax: +1-317-895-5652
[business activities since 1990: analytical services in AFM, AFM probes,
consulting, training, calibration and test specimens,
calibration and measurement software, used NanoScope equipment.]
=============================================


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From: microscopy.listserver-at-gmail.com
Date: May 1, 2016 at 5:58:19 PM CDT
Subject: [Microscopy] low vacuum pump

Contents Retrieved from Microscopy Listserver Archives
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I'm working with a wafer inspection system that uses vacuum to clamp the
wafer. The manufacturer's specifications call for a modest vacuum:

low vacuum: 150 torr (-24" Hg)

flow rate: { 1 cfm (28 l/m)

Actual flow will be intermittent.



What type of pump would be suitable for this application?

I welcome suggestions for specific models and suggestions for how to
configure the pump.

regards,
Don
=============================================
Don Chernoff, Ph.D., President
Advanced Surface Microscopy, Inc.; 3250 N. Post Rd., Suite 120;
Indianapolis IN 46226 USA
E-Mail: donc-at-asmicro.com www.asmicro.com
Voice: +1-317-895-5630 Toll free: +1-800-374-8557 (in USA & Canada)
Fax: +1-317-895-5652
[business activities since 1990: analytical services in AFM, AFM probes,
consulting, training, calibration and test specimens,
calibration and measurement software, used NanoScope equipment.]
=============================================


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From: microscopy.listserver-at-gmail.com
Date: April 29, 2016 at 7:05:23 PM CDT
Subject: [Microscopy] viaWWW:Fume hood extension arm

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X-from: rebecca.jackson-at-utsouthwestern.edu

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Email: rebecca.jackson-at-utsouthwestern.edu
Name: Rebecca Jackson

Organization: UT Southwestern

Title-Subject: [Filtered] Fume hood extension arm

Message: Hey all- We are looking into getting a fume hood extension/extraction arm in our EM lab,
hopefully making it easier and safer for cutting tissues in fix using the dissecting scope. We hope
to do this on the bench with the arm right next to the dish containing the tissue and fix while
using the dissecting scope. Does anyone have experience with these? Could I get some info on what
you have or if these even work well for harsh chemicals? Or do you do/use something totally
different....? Thanks all!!

-Bec.

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From: microscopy.listserver-at-gmail.com
Date: April 29, 2016 at 1:56:12 PM CDT
Subject: [Microscopy] Question about soft polymers, toxins, beam sublimation and what

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Hello,


We have a student, that would like to work with a polymer that if 100% polymerized would be ok, but seldom are polymer's 100% polymerized. In other physical states this polymer is toxic.


They hope to make this into a thin film, and use it in x-ray, SEM and TEM.


The toxin/ sensitizer is ethylviologen (ethyl iodide), a bio based substrate known to be a sensitizer, the polymer has the base of Polyvinylbenzylchloride, 4-4-bipyridine, and could include ethylviologen monomers and dipyridyl monomers.




SUGGESTED PRECAUTIONS ALREADY:
Vacuum for a week, mild heat to help gas off, buying own sample stubs and bringing already prepped.
Test just plain polybenzylchloride to watch for sublimation.




Desired instruments of use: SEM, TEM, X-ray




QUESTIONS:


* Even if vacuum is pre-applied for as long as a week in their own lab, will such a product gas out into the TEM causing all the insides to be contaminated?

* Even if vacuum is not a problem, what about sublimation of the polymer by the beam?

* What are your standard precautions for soft polymers, especially those that are toxic? What are the limits of materials you allow in your EM scopes?


Thanks for any help,

Lou Ann







{ { { { { { { { { { { { { { { { {} } } } } } } } } } } } } } } }
Lou Ann Miller
Biological Electron Microscopy
Frederick Seitz Materials Research Laboratory
Lab Room 125 Office 374 MRL
104 South Goodwin Ave
Urbana, IL 61801
Campus mail code: MC-230
Hours: 7am-3:30 pm

217-244-1567
lamiller-at-illinois.edu
http://mrl.illinois.edu



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From: microscopy.listserver-at-gmail.com
Date: April 29, 2016 at 1:18:00 PM CDT
Subject: [Microscopy] Photo printers

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Listers,

I haven't had to buy a photo printer for micrographs for some years.
What's the current best? HP doesn't make the Photosmart series anymore,
and the Epson Sure color series specs look good, but ... ?
I need both color and greyscale.
Maybe the Epson P600?
Thanks.

Phil
--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

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From: microscopy.listserver-at-gmail.com
Date: April 29, 2016 at 11:24:15 AM CDT
Subject: [Microscopy] Fwd: LaB6 Update: Could use some suggestions

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Note! Please do *not* use "Reply"! Send any response to the list or to
John L. Grazul {jlg98-at-cornell.edu} . I'm sending this for a colleague
locked behind a Cornell Curtain. Phil

LaB6 enthusiasts! This afternoon I am looking at 4 Kimball filaments in
the FIB. Some had a dignified normal death, others an untimely and less
dignified death. What we are going to look at is the elemental
differences between them, from the La right to the base. We are going to
do slice and views and look for defects. Is there anything else you guys
want us to investigate? Let me know and your dreams will come true, we
are the Disneyland of microscopy.

I have been contacted by one of the manufacturers, they too are
interested in the data we get. So things are moving and hopefully we
will be getting good product in the near future.

Next on my agenda will be the quality and performance of FEG tips. How
have your FEG tips been lately?? We at Cornell have tales to tell. Send
me your tales of woe or post them, but lets just say our experience
lately has been similar to our issues with LaB6.

Thanks for interacting, and if we dont keep the suppliers on their
toes, our down time because of poor product will just be expected rather
than a surprise.

jg


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From: microscopy.listserver-at-gmail.com
Date: April 29, 2016 at 7:33:04 AM CDT
Subject: [Microscopy] viaWWW:Project Scientist Series Electron Microscopy Scientific Staff

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Email: jzheng-at-uci.edu
Name: Jian-Guo Zheng

Organization: IMRI UCI

Title-Subject: [Filtered] Project Scientist Series Electron Microscopy Scientific Staff -positions
open at UC Irvine

Message: For details, please visit https://recruit.ap.uci.edu/apply/JPF03381

RECRUITMENT PERIOD
Open April 20th, 2016 through June 30th, 2016
DESCRIPTION

Project Scientist Series -Electron Microscopy Scientific Staff

Salary-Commensurate with experience

POSTING DATE: APRIL 20, 2016 CLOSING DATE: OPEN UNTIL FILLED

DESCRIPTION
Manage the day-to-day operations and help guide the strategic build-out of the new transmission
electron microscopy (TEM) facility at the UC Irvine Materials Research Institute (IRMI). IMRI has a
large and growing user community of over 300 academic and industry users, with advanced materials
characterization instruments that include state-of-the-art TEMs, scanning electron microscopes
(SEMs), focused ion beam (FIB) systems, and a Kratos AXIS Supra surface science instrument (for
details see http://lexi.eng.uci.edu). IMRI has recently built a new TEM facility housing five major
instruments, including four high-end TEMs (Nion UltraSTEM 200 HERMES, JEOL JEM-ARM300CF Grand ARM,
JEM-2800, and JEM-2100F Cryo-TEM) and a dual-beam FIB (Tescan GAIA-3 XMH FIB-SEM). IMRI will be the
first lab in the Americas with the newly introduced JEOL Grand ARM and the Nions high-performance
UltraSTEM 200 HERMES, which provides an energy resolution for electron energy loss spectroscopy
(EELS) of 7 meV or better. Both the Grand ARM and the 2100F Cryo-TEM will be equipped with Gatans
K2 cameras. IMRI is also equipped with a variety of TEM in situ holders and specimen preparation tools.
Two TEM scientific staff positions are available: (1) TEM specialist for materials science
applications and (2) Cryo-TEM specialist for biological and soft materials.
The successful applicant must hold PhD or equivalent degrees in the areas of Materials Science,
Physics, Chemistry or biology, and be TEM experts with at least five years of in-depth, hands-on
experience with TEM/STEM imaging, spectroscopy, TEM sample preparation, and maintenance of TEM
equipment. Applicants who are merely users of TEM will not be considered. The applicant must have
mastery of modern TEM/STEM instrumentation and methods, data collection, analysis, and
interpretation. Candidates with experience operating user facilities and expertise with advanced
TEM/STEM imaging, spectroscopy, and in-situ microscopy techniques are of particular interest. Duties
include: operate, maintain, and improve the TEMs; work with the IMRI leadership to identify and
obtain accessories and additional equipment for a phased build-out of the TEM facility; train,
supervise, and work directly with users to carry out research; co-manage all aspects of day-to-day
IMRI operations; participate in teaching and outreach activities; promote IMRI on and off campus;
secure grants, perform original research, attend scientific conferences, and publish scientific
research in peer-reviewed journals.
The University of California, Irvine is an Equal Opportunity/Affirmative Action Employer advancing
inclusive excellence. All qualified applicants will receive consideration for employment without
regard to race, color, religion, sex, sexual orientation, gender identity, national origin,
disability, age, protected veteran status, or other protected categories covered by the UC
nondiscrimination policy.
TO LEARN MORE AND APPLY
Apply by submitting your application to our online RECRUIT system at:
https://recruit.ap.uci.edu/apply/JPF03381
More information about this recruitment: www.calit2.uci.edu
JOB LOCATION
Irvine, CA
REQUIREMENTS
DOCUMENTS
Curriculum Vitae - Your most recently updated C.V.
Cover Letter
Statement of Research
Statement of Teaching (Optional)
Statement of Contributions to Diversity - Statement addressing how past and/or potential
contributions to diversity will advance UCI's Commitment to Inclusive Excellence.
Misc / Additional (Optional)
REFERENCES
3-5 letters of reference required
HOW TO APPLY
1. Create an ApplicantID
2. Provide required information and documents
3. If any, provide required reference information

To apply these positions, please visit https://recruit.ap.uci.edu/apply/JPF03381
Thanks!

Jian-Guo Zheng, PhD, FRMS
IMRI Director of Facilities
Irvine Materials Research Institute
University Of California, Irvine
3421 Calit2 Building
Irvine, CA 92697-2800
USA
Telephone: 949-824-0441 (office), 949-468-9980 (cell)
Fax: 949-824-8195
Email: jzheng-at-uci.edu


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From: microscopy.listserver-at-gmail.com
Date: April 29, 2016 at 5:25:00 AM CDT
Subject: [Microscopy] EMS850 CPD use

Contents Retrieved from Microscopy Listserver Archives
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Gary:

I've already sent you a manual for the EMS 850 CPD but can give you some pointers that are not included in the manual.

What CO2 tank size do you use? Typically your gas supplier will deliver a J-size tank but it MUST have a "Dip tube or Siphon tube" for any CPD to function. The siphon tube draws liquid Co2 from the bottom of the tank, without this you would only draw gas.

What support items do you use? Plastic waste containers to contain Alcohol waste. Small plastic flowmeter to measure the flow of Co2 out of the pressure vessel during the final stage of drying. There are several types of sample holders depending on application, Everything from bulk samples to coverslip holders are in the EMS 850 section of our catalog.

This unit comes with a SST reinforced, 1500psi certified transfer hose. If you didn't get one with the used 850, contact me directly for a replacement.

How automatic is the system? The EMS 850 is semi-automatic. See the details of operation in the manual.

For putting bio specimens into the SEM, how do you factor-in this CPD before going into the chamber? Not sure I understand what the question is. All biological samples must be dried* before coating or insertion into the SEM


Al Coritz (acoritz-at-emsdiasum.com)
Applications & Service Manager
EMS Technical Service

www.emsdiasum.com
800-523-5874
1560 Industry Road
Hatfield, PA 19440


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From: microscopy.listserver-at-gmail.com
Date: April 28, 2016 at 8:45:51 AM CDT
Subject: [Microscopy] Fwd: LaB6 update and some suggestions

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Note! Please do *not* use "Reply"! Send any response to the list or to
John L. Grazul {jlg98-at-cornell.edu} . I'm sending this for a colleague
locked behind a Cornell Curtain. Phil

Message:
Fellow microscopists I have a follow up on our LaB6 issue and a
suggestion that should work from one of our microscopy luminaries.

There is correspondence going out to Denka and Kimball regarding our
issues, with our concerns and observations attached, from one of the
major suppliers of microscopy consumables. Hopefully there will be some
movement or at least an acknowledgement that Oh yeah, we did change our
La supplier and welding technique and who we are outsourcing
manufacturing to. Dont think you are the only one having issues guys!
Throw something up on the list and at the least you will get that
reality check that you are not losing your mojo, or the reality check
that indeed you ARE losing your mojo.

Regarding the manual alignment phenomena; if you recall, we align the
Lab6 on the bench as usual, put the gun in the scope, heat it up and the
deflectors cant center the tip, we max out. Take the gun out and the
manual alignment looks great. Henk Colijn theorizes that the resistance
is not the same on the posts so as they heat up the tip gets pulled to
one side or the other. Earl Kirkland suggested that we anneal the
filament before we manually align, which is what we do for our cold
FEGs. I gave it a go with my latest Lab6: ran the HT to 120kV and ran a
dribble current through the crystal over the weekend, getting it good
and hot, manually aligned and so far so good. BUT we should not have to
do this because in the recent past we never had to do this. In our case
it is more downtime which ultimately adds to the cost of the filament.

So guys, I will keep you posted on what happens, any other observations
or suggestions please pass on to our community. Some issues may seem
trivial but we all have a common bond, and our collective mojo is a
pretty awesome force.

jg


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From: microscopy.listserver-at-gmail.com
Date: April 28, 2016 at 7:25:04 AM CDT
Subject: [Microscopy] viaWWW:Current EM Techniques Workshop on May 24 & 25

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Organization: University of Maryland, Baltimore

Title-Subject: [Filtered] Current EM Techniques Workshop on May 24 & 25

Message: Dear Colleagues,

We would like to remind you that the registration of the UMB Current EM Techniques Workshop
(05/24-05/25) is OPEN. The purpose of this annual workshop is to introduce participants to new
electron microscopy techniques and instruments via live demonstration and open discussion. The
focus of this years workshop is Correlative LM & EM. The workshop will include oral presentations,
Tips-and-Tricks Technology forums on the first day and live instrument demonstration on the second
day. Dr. Lucy Collison of Francis Crick Institute and Richard Schalek of Harvard University will be
the keynote speakers. Demo instruments will feature ATUMtome of RMC-Boeckeler, ASP-1000-IGL of
Microscopy Innovations, Pelco Biowave Pro Microwave System of TedPella, Cryo EM sample preparation
of Leica Microsystems, Correlative Array Tomography of Zeiss, Polyo III, Lynx Tissue Processor of
EMS, Aerosurf Atmospheric SEM of Hitachi/Angstrom.

A joint dinner event with the Chesapeake Microscopy and Microanalysis society (CMMS) on May 24th
provides opportunities for social and scientific exchange among workshop participants and CMMS
members.

More information: http://www.dental.umaryland.edu/2016currentemtechniquesworkshop/

Registrations: https://umbcurrentemtechniqueworkshop.eventbrite.com

Inquiry: mailto:coreimaging-at-umaryland.edu?subject=CMMS%20dinner

We look forward to seeing you in May.


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From: microscopy.listserver-at-gmail.com
Date: April 28, 2016 at 2:56:20 AM CDT
Subject: [Microscopy] preparation of fat in mouse liver specimen

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Dear colleagues biologists,

I am preparing mouse livers for TEM the classical way: fixation glutar+Formaldehyde, Osmium post-fixation, embedding in Epon. Some liver samples are loaded with fat, mainly due to a treatment the mice received. The sample were left several weeks in fixative at 4*C before processing so extraction of at least some fat would not be a surprise.

Now on semithin sections stained with methylene blue/Azur II the fat globules have a greenish blue appearance. I thought it was due to the presence of osmium. However, when I observe ultrathin sections of the same samples in TEM, the fat globule are empty, I see nothing, it is only resin inside so they are probably extracted.
My question is: how can the fat be colored in semi-thin sections and empty/extracted in ultrathin section?
How do you explain de greenish blue color with methylene blue/azur II?

Regards,
Stephane


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From: microscopy.listserver-at-gmail.com
Date: April 27, 2016 at 8:31:09 PM CDT
Subject: [Microscopy] EMS850 CPD use

Contents Retrieved from Microscopy Listserver Archives
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Greetings to all Listers.

I'm tring to get a used EMS850 CPD up and running and wonder what
others' experiences have been with this unit. This unit is before the
version with a single dial gauge and digital readout.

What CO2 tank size do you use?
What support items do you use?
What hose works for you?
How automatic is the system?

For putting bio specimens into the SEM, how do you factor-in this CPD
before going into the chamber?

All input is appreciated.

TIA,
gary g.

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From: microscopy.listserver-at-gmail.com
Date: April 27, 2016 at 4:03:43 PM CDT
Subject: [Microscopy] Field canceling systems in a Talos enclosure

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Everyone,
We are planning a field canceling system for the room where we will house an FEI Talos. There is the possibility of building the coils into the microscope enclosure - has anyone done that? Looking to hear experiences.

Thanks



Chris

Christopher J. Gilpin Ph.D.
Director, Life Science Microscopy Facility
Purdue University
Whistler Hall of Agriculture Research, Room S052
170 S. University St
West Lafayette, IN 47907
765-494-7750
gilpin-at-purdue.edu
lsmf-at-purdue.edu reaches everyone in the facility



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From: microscopy.listserver-at-gmail.com
Date: April 27, 2016 at 7:03:35 AM CDT
Subject: [Microscopy] viaWWW: Electron microscopy position open New York University Abu

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X-from: james.weston-at-nyu.edu

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Email: james.weston-at-nyu.edu
Name: James Weston

Organization: New York University Abu Dhabi

Title-Subject: [Filtered] Electron microscopy position open

Message: Hello,
We have recently posted a position for a research instrumentation specialist in electron microscopy.

This is a permanent staff position. The individual would be responsible for three electron
microscopes (SEM, TEM, Dual beam).

http://nyuad.nyu.edu/en/about/careers/administration-staff/2016/04/research-instrumentation-specialist---electron-microscopy.html

Please pass on the announcement to colleagues who are not subscribers to this list service

Thanks!
James

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From: microscopy.listserver-at-gmail.com
Date: April 26, 2016 at 9:30:27 PM CDT
Subject: [Microscopy] viaWWW:EELS and EFTEM Workshop at Harvard University

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Email: jhyun-at-gatan.com
Name: John Hyun

Organization: Gatan, Inc.

Title-Subject: [Filtered] EELS and EFTEM Workshop at Harvard University

Message: 10:00 am - 6:00 pm, May 24, 2016
Center for Nanoscale Systems (CNS), Room 303, Laboratory for Integrated Science and Engineering
(LISE) Building, Harvard University, MA

This workshop combines presentations and extensive hands-on training on advanced EELS and EFTEM
techniques and applications in the (S)TEM microscope. Practical experience with EELS is highly
recommended. Participants will learn best practices to set up and optimize their EELS hardware and
experimental protocols so they can capture and extract the maximum amount of compositional and
chemical information from their TEM samples.

Topics include:

-Advanced EELS acquisition techniques extended dynamic range and DualEELS
-Advanced EELS processing techniques, MLLS and NLLS fitting
-Aberration correction
-Quantification using EELS
-Ultrafast compositional mapping
-High energy resolution chemical mapping and identification of different chemical phases
-High energy resolution EELS STEM analysis
-Atomic resolved level analysis using EELS
-How to best optimize the spectrometer and increase the sensitivity
-How to set up the microscope to get the best EELS results

This event is free to registered participants. Register online at:
http://www.gatan.com/company/events/advanced-eels-and-eftem-workshop-harvard-university



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From: microscopy.listserver-at-gmail.com
Date: April 26, 2016 at 9:28:43 PM CDT
Subject: [Microscopy] viaWWW: NIH staff scientist position

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Email: kacharb-at-nidcd.nih.gov
Name: Bechara Kachar

Organization: NIH

Title-Subject: [Filtered] staff scientist position

Message: Staff Scientist Position

The Laboratory of Cell Structure and Dynamics (LCSD) in the Division of Intramural Research (DIR),
National Institute on Deafness and Other Communication Disorders (NIDCD), National Institutes of
Health (NIH), seeks a Staff Scientist.

The LCSD seeks an integrated molecular understanding of the architecture, dynamics, function, and
renewal of specialized cellular structures underlying the mechanosensory function of auditory and
vestibular sensory hair cells. Current projects at the LCSD involve the use of a combination of cell
biology approaches with super-resolution fluorescence microscopy and cryo-electron microscopy to
examine the molecular architecture of the actin-based sensory stereocilia and their
mechanotransduction molecular complex.

The NIDCD intramural program is comprised of a highly interactive and dynamic group of scientists at
the forefront of research on communication disorders. The LCSD is located in the newly constructed,
multi-disciplinary Porter Neuroscience Research Center on the main NIH campus in Bethesda, Maryland.

The LCSD Staff Scientist serves as a laboratory leader who contributes to mentoring of postdoctoral
fellows and other laboratory staff and trainees. The position requires outstanding interpersonal
skills, teamwork, and excellent oral and written communication abilities. The successful candidate
should have a doctoral degree, postdoctoral research experience, and an outstanding publication
record. Requisite expertise includes molecular and cellular biology, and either advanced
fluorescence microscopy imaging or CryoEM. Salary is commensurate with experience.

Applicants should submit their curriculum vitae with bibliography, statement of interest, and names
and contact information for three references to Bechara Kachar at kacharb-at-nidcd.nih.gov.
Applications will be reviewed starting May 15, 2016, and accepted until the position is filled. HHS,
NIH, and NIDCD are Equal Opportunity Employers.
For more information about the LCSD and NIDCD, please visit our website:
https://www.nidcd.nih.gov/about/staff/bechara-kachar-md
https://www.nidcd.nih.gov/research/labs/section-structural-cell-biology

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From: microscopy.listserver-at-gmail.com
Date: April 23, 2016 at 6:41:51 AM CDT
Subject: [Microscopy] viaWWW:SEM Imaging & Processing

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Email: akgadde-at-mix.wvu.edu
Name: akshitha

Organization: West Virginia University

Title-Subject: [Filtered] Image Processing

Message: Can someone help me understand in detail how the image processing works in background in a
scanning electron microscope.
How an image is produced from the current signal input to the image processing input in a scanning
electron microscope.

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From: microscopy.listserver-at-gmail.com
Date: April 21, 2016 at 2:44:57 PM CDT
Subject: [Microscopy] Attendees, Competitors and Vendors - University of Illinois

Contents Retrieved from Microscopy Listserver Archives
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Announcing the Fall Biological Conference at the University of Illinois Materials Research Lab.



DATE: November 2-3, 2016

Conference Title: Diverse Fields of Study... Steps toward Medicine.
________________________________________
An apropos title, as UIUC is starting up its own medical school, MD/PHD with the PhD in Bioengineering.




Attendee registration to open in June and is open to scientists from all over.
=========================================================

Vender registration is open NOW.
==========================


Student and Post Doc Presentation Divisions Competition:
=============================================
up to 4 post docs, up to 9 students
Register from June to October 1st, and upload 1/2 page abstract that will be judged by a panel for acceptance. Winners receive monetary awards, registration waved, and their image on our website for several months.
Students as well as post docs from other institutions are welcome.



Invited Speakers:
==============


Our student competition: up to 4 post docs, up to 9 students.

Dr. Catherine Murphy, Chemistry, MRL associate director
Gold Nanoparticles: Physics, Chemistry, Biology and Ecology

Adele Akhtar of Psionic, The Future of Upper Limb Prosthetic Devices,
Doctoral Candidate Adele Akhtar owns a company that works with prosthetics that can sense touch pressure.

Dr. Ralph Nuzzo - Chemistry/ MRL about Hydrogel and cells

Dr Amy Wagoner - Johnson MechSci Biomaterials, bone implants

Dr Rashid Bashir - Bioengineering.

Dr. Mauro Sardela our new MRL director of the MRL Central Research Facilities, covering what our facility is all about.

Also a representative from our Safety department, topic to be determined.






VENDORS:
===========

This year we have 2 full days of conference,and we have some treats for our vendors. the second day. The vendor area will be open to the public, so this will give you a chance to set up demos and schedule tutorials at your table. Also, you can invite your contacts to come and visit you, regardless of if they have registered. So the second day is public and yours to add visitors, tutorials etc, as you may wish.




The second thing we would like to do for you is give you a chance to speak at the conference. When registering, you can upload a one page abstract to submit, images welcome. This should be in the form of a teaching tutorial. We were able to carve out some time, the 4 best panel judged abstracts will be able to give a 30 minute talk on the second day.

Abstract Deadline for the abstract submission is July 4th, 2016, and folks will know one way or the other by July 10th.

Registration deadline is closer to the meeting, but will end if all tables are full.

There is no fee for the chance to speak, and for 2 full days of tables, the price is $250/ table.

Registration is now open at: "MRL Biological Conference 2016 Exhibitor Registration"
https://my.mrl.illinois.edu/eventreg/register.asp



Thanks! More information at the start of registration for attendees is to come.

Lou Ann


{ { { { { { { { { { { { { { { { {} } } } } } } } } } } } } } } }
Lou Ann Miller
Biological Electron Microscopy
Frederick Seitz Materials Research Laboratory
Lab Room 125 Office 374 MRL
104 South Goodwin Ave
Urbana, IL 61801
Campus mail code: MC-230
Hours: 7am-3:30 pm

217-244-1567
lamiller-at-illinois.edu
http://mrl.illinois.edu


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From: microscopy.listserver-at-gmail.com
Date: April 21, 2016 at 1:09:48 PM CDT
Subject: [Microscopy] Follow up to LaB6 Issues

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

LaB6 users, I am not losing my touch and there is no LaB6 death vortex
hanging over Ithaca! I have a whole slew of responses that concur with
the issues I have been having. If any of you who are having the same
issue please get in touch with me because if we dont tell the vendors
and the suppliers that their product has changed for the worse they will
still keep selling us garbage because either they dont know or just
dont care. BTW, not one retort from Kimball or Denka
yet.

I think all we want is a LaB6 that shows us a Maltese Cross, doesnt
drift X-Y & Z to the point that the deflectors cant compensate (this is
a common issue, you take the gun out and the tip still looks perfectly
centered, you apply a current and it tilts toward the Emerald City), and
a long life; just the way they used to be ten years ago.

I cant make it to M&M this year or next, thats when the ankle bracelet
comes off, but this may be a good topic for a round table because we the
users are not imagining things.

Send me some more horror stories, I will compile them and post them or
you guys just throw them up on the Microscopy list, do not respond to
Phil, I may owe him a keg if you reply to him.

jg

John L. Grazul {jlg98-at-cornell.edu}


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From: microscopy.listserver-at-gmail.com
Date: April 21, 2016 at 10:48:08 AM CDT
Subject: [Microscopy] LaB6 Issues anyone?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We too have had problems with Denka LaB6 on our FEI T20. Short life and bad drift. We have to take the wehnelt out several times to re-center the LaB6 as it runs out of gun tilt. We are experimenting with whether to just turn down the filament current for overnight or if it is best to turn it off completely. After a long spell of just turning it lower we are now turning it on in the morning and off at the end of the day. Its early days but I think we have better lifetime. Next time we change filament we have plans to try a different cathode assembly.


Chris

Christopher J. Gilpin Ph.D.
Director, Life Science Microscopy Facility
Purdue University
Whistler Hall of Agriculture Research, Room S052
170 S. University St
West Lafayette, IN 47907
765-494-7750
gilpin-at-purdue.edu
lsmf-at-purdue.edu reaches everyone in the facility


-----Original Message-----
X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu]
Sent: Wednesday, April 20, 2016 4:19 PM
To: Gilpin, Christopher J {gilpin-at-purdue.edu}

Note! Please do *not* use "Reply"! Send any response to the list or to John L. Grazul {jlg98-at-cornell.edu} . I'm sending this for a colleague locked behind a Cornell Curtain. Phil

Message:
Fellow microscopists it has been a long time since I have posted mainly because anything that comes out of Cornell is un-postable for really good reasons I assume. The huge issue I have been having for the past couple of years is the quality, reliability and life span of my LaB6 sources. Now this is not an issue with just one scope or just one vendor. Tungsten filaments work fantastic and hit all the numbers perfectly in both of our 120kV Tecnais. When we put a LaB6 in really weird things happen. Before you get suspicious and think we just started up with LaB6, this is all that we have ever used on our tools, I just had to test the scopes with W to make sure I wasn't imagining things. We need LaB6 because of the brightness and hours of use; each scope is used
40-50 hr/week. So much for the introduction, now on to the issues:

1. I have not seen a Maltese cross on either a Kimball or Denka in 5 years or so, or about 5- 8 filaments, on either of my Tecnais. The engineers and I just undersaturate and go for max brightness.
2. Filament life used to be 2500-3000 hours, the last five tips have flamed out around 500 hours. BTW the vacuum has been rock steady in both scopes.
3. I have had two tips fail due to micro cracks in the carbon crucible.
4. The last five tips drift for the first 100-200 hours, and we are talking X-Y and Z. This is to the point that the deflectors can't compensate and I have to break vacuum and re-center manually.

5. My 24 Volt power supply for the filament is fine, swapped them around my scopes with the same result.

If the darn things didn't cost nearly $900.00 I might tolerate this. I have to give high marks to EMS, they have been listening to my complaints and have made good on the tips that have failed out of the box. But the quality of the product I really have questions about. Has Kimball changed their carbon supplier, is it softer? Why are there cracks? And the last question is have any of you guys had the same or similar issues? And the absolutely final question is am I missing something? After 30+ years of ripping scopes apart I might just have lost the magic touch or there is a vortex of Lab6 instability issues in Ithaca.

Please contact me on or off line:
John L. Grazul {jlg98-at-cornell.edu}
--

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From: microscopy.listserver-at-gmail.com
Date: April 21, 2016 at 10:08:03 AM CDT
Subject: [Microscopy] Re: LaB6 Issues anyone?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

John

We to have been having the same problem with Denka LaB6 filaments on a
JEM-3010. We have taken the last two out after aligning them because
they appear to be tilted only to find that they are perfectly aligned!

Over time the last one got better and we got closer to the Maltese cross
. We have not seen a sharp decline in lifetime however.

Regards

Alan

On 4/20/2016 3:00 PM, oshel1pe-at-cmich.edu wrote:
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Note! Please do *not* use "Reply"! Send any response to the list or to
John L. Grazul {jlg98-at-cornell.edu} . I'm sending this for a colleague
locked behind a Cornell Curtain. Phil

Message:
Fellow microscopists it has been a long time since I have posted mainly
because anything that comes out of Cornell is un-postable for really
good reasons I assume. The huge issue I have been having for the past
couple of years is the quality, reliability and life span of my LaB6
sources. Now this is not an issue with just one scope or just one
vendor. Tungsten filaments work fantastic and hit all the numbers
perfectly in both of our 120kV Tecnais. When we put a LaB6 in really
weird things happen. Before you get suspicious and think we just started
up with LaB6, this is all that we have ever used on our tools, I just
had to test the scopes with W to make sure I wasnt imagining things. We
need LaB6 because of the brightness and hours of use; each scope is used
40-50 hr/week. So much for the introduction, now on to the issues:

1. I have not seen a Maltese cross on either a Kimball or Denka in 5
years or so, or about 5- 8 filaments, on either of my Tecnais. The
engineers and I just undersaturate and go for max brightness.
2. Filament life used to be 2500-3000 hours, the last five tips have
flamed out around 500 hours. BTW the vacuum has been rock steady in both
scopes.
3. I have had two tips fail due to micro cracks in the carbon crucible.
4. The last five tips drift for the first 100-200 hours, and we are
talking X-Y and Z. This is to the point that the deflectors cant
compensate and I have to break vacuum and re-center manually.

5. My 24 Volt power supply for the filament is fine, swapped them around
my scopes with the same result.

If the darn things didnt cost nearly $900.00 I might tolerate this. I
have to give high marks to EMS, they have been listening to my
complaints and have made good on the tips that have failed out of the
box. But the quality of the product I really have questions about. Has
Kimball changed their carbon supplier, is it softer? Why are there
cracks? And the last question is have any of you guys had the same or
similar issues? And the absolutely final question is am I missing
something? After 30+ years of ripping scopes apart I might just have
lost the magic touch or there is a vortex of Lab6 instability issues in
Ithaca.

Please contact me on or off line:
John L. Grazul {jlg98-at-cornell.edu}


--
Alan W Nicholls, PhD
Associate Director, RRC
Director of Research Service Facility (Electron Microscopy)
Research Resources Center - East (M/C 337)
Room 110, Science and Engineering South Building
The University of Illinois at Chicago
845 West Taylor St
Chicago, IL 60607-7058

Tel: 312 996 1227

Web site http://www.rrc.uic.edu/ems
Wiki site https://wiki.rrc.uic.edu/wiki/EMS


==============================Original Headers==============================
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From: microscopy.listserver-at-gmail.com
Date: April 21, 2016 at 6:13:26 AM CDT
Subject: [Microscopy] viaWWW: Research Fellow (Microscopy of thin film materials) @ Monash

Contents Retrieved from Microscopy Listserver Archives
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X-from: matthew.weyland-at-monash.edu

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Email: matthew.weyland-at-monash.edu
Name: Matthew Weyland

Organization: Monash University

Title-Subject: [Filtered] Research Fellow (Microscopy of thin film materials)

Message: The following position is currently available:

Research Fellow (Microscopy of thin film materials);

http://www.jobs-monash.jxt.net.au/academic-jobs/research-fellow-microscopy-of-thin-film-materials-/627753

This position is based in both the Monash Centre for Electron Microscopy (MCEM) and The Department
of Materials Science and Engineering at Monash University in Melbourne, Australia. The research is
part of a close collaboration with the School of Materials Science and Engineering, The University
of New South Wales (UNSW), Sydney.

https://platforms.monash.edu/mcem/
http://www.eng.monash.edu.au/materials/
http://www.materials.unsw.edu.au/

Application Closing Date - Thursday 12 May 2016, 11:55pm AEST

For details of the research project, please contact directly.

A/Prof. Matthew Weyland
Monash Centre for Electron Microscopy and Department of Materials Science and Engineering
10 Innovation Walk, Clayton Campus
Monash University
Clayton VIC 3800 Australia
T: +61 3 9905 9026
E: matthew.weyland-at-monash.edu
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From: microscopy.listserver-at-gmail.com
Date: April 20, 2016 at 10:36:18 PM CDT
Subject: [Microscopy] LaB6 Issues anyone?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Henk
Sorry, my experience is only with Tungsten but what about if you position
the cathode a little bit shifted to the opposite direction you suspect it
shifts after warming. So you may correct the problem and verify your theory
at the same time..
regards
yorgos


Dr Yorgos Nikas
Athens Innovative Microscopy
Skra 36 Voula 16673 GREECE

Tel/fax +30 210 8957677
mobile +30 6945 107477
www.eikonika.net www.aim.cat
*************************************



-----Original Message-----
X-from: colijn.1-at-osu.edu [mailto:colijn.1-at-osu.edu]
Sent: Thursday, April 21, 2016 3:56 AM
To: eikonika-at-otenet.gr

Hi John, et al,

I've had some issues over the past several years with the Kimball Physics
LaB6 cathodes. (I'm not sure which brand you are using.) After
meticulously setting the depth and centering the cathode, I run out of gun
tilt when warming it up. After pulling it back out of the scope, I find
that the cathode is still perfectly centered in the wehnelt. I often go
through 3 or 4 iterations before I can get a usable setting of the cathode,
each time finding that it looks perfectly centered outside of the scope.

My suspicion is that the legs have slightly different resistances and that
one leg warms up more than the other pushing the cathode to the side. When
the cathode cools back down, the tip recenters itself. This theory is
pretty hard to verify since I can't directly observe the tip while it is
warm. If I can get the tip centered enough I do see the "maltese cross"
figure though sometimes it's a bit off center.

One thing that may affect your lifetime is the filament limit for
saturation. I have noticed that if I initially set the cathode to be just
saturated, then check it 20-30 minutes later I find I can back the filament
drive off by 1 or 2 clicks and it is still saturated. I assume that as the
components warm up, the saturation point changes slightly. I do have to tell
the students that this is the correct way to operate so that they don't
reset the limit themselves. So, I now set my filament limit to be at least
one click undersaturated. This way, as it warms up, it will bring itself to
the proper saturation point. Note that the Kimball Physics documents
indicate that the evaporation rate will triple in going from 1800K to 1850K.
This will bring the lifetime from ~2000 hours down to a 600-700 hour range.

If the tip isn't oxidized by one of the students, I can still get close to
2000 hours from a cathode. I did have one student destroy one in 20 minutes
by letting the cold trap warm up while it was hot. (I was NOT
happy.)
Kimball Physics has some useful info in the technical documents on their web
site.

Cheers,
Henk

--------------------

Hendrik O. Colijn
Center for Electron Microscopy and AnalysiS The Ohio State University
1305 Kinnear Rd, Suite 100, Columbus, OH 43212

colijn.1-at-osu.edu 614/643-3458
cemas.osu.edu {about:cemas.osu.edu}

"Time is that quality of nature which keeps things from happening all at
once." (Ray Cummings - 1922)
Lately it doesn't seem to be working.



------ Original Message ------
X-from: oshel1pe-at-cmich.edu
To: colijn.1-at-osu.edu
Sent: 4/20/2016 4:01:46 PM

Note! Please do *not* use "Reply"! Send any response to the list or to
John L. Grazul {jlg98-at-cornell.edu} . I'm sending this for a colleague
locked behind a Cornell Curtain. Phil

Message:
Fellow microscopists it has been a long time since I have posted mainly
because anything that comes out of Cornell is un-postable for really
good reasons I assume. The huge issue I have been having for the past
couple of years is the quality, reliability and life span of my LaB6
sources. Now this is not an issue with just one scope or just one
vendor. Tungsten filaments work fantastic and hit all the numbers
perfectly in both of our 120kV Tecnais. When we put a LaB6 in really
weird things happen. Before you get suspicious and think we just
started
up with LaB6, this is all that we have ever used on our tools, I just
had to test the scopes with W to make sure I wasn?t imagining things.
We
need LaB6 because of the brightness and hours of use; each scope is
used
40-50 hr/week. So much for the introduction, now on to the issues:

1. I have not seen a Maltese cross on either a Kimball or Denka in 5
years or so, or about 5- 8 filaments, on either of my Tecnais. The
engineers and I just undersaturate and go for max brightness.
2. Filament life used to be 2500-3000 hours, the last five tips have
flamed out around 500 hours. BTW the vacuum has been rock steady in
both
scopes.
3. I have had two tips fail due to micro cracks in the carbon crucible.
4. The last five tips drift for the first 100-200 hours, and we are
talking X-Y and Z. This is to the point that the deflectors can?t
compensate and I have to break vacuum and re-center manually.

5. My 24 Volt power supply for the filament is fine, swapped them
around
my scopes with the same result.

If the darn things didn?t cost nearly $900.00 I might tolerate this. I
have to give high marks to EMS, they have been listening to my
complaints and have made good on the tips that have failed out of the
box. But the quality of the product I really have questions about. Has
Kimball changed their carbon supplier, is it softer? Why are there
cracks? And the last question is have any of you guys had the same or
similar issues? And the absolutely final question is am I missing
something? After 30+ years of ripping scopes apart I might just have
lost the magic touch or there is a vortex of Lab6 instability issues in
Ithaca.

Please contact me on or off line:
John L. Grazul {jlg98-at-cornell.edu}
--

==============================Original
Headers==============================
6, 33 -- From oshel1pe-at-cmich.edu Wed Apr 20 14:58:44 2016
6, 33 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116])
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From: microscopy.listserver-at-gmail.com
Date: April 20, 2016 at 10:30:31 PM CDT
Subject: [Microscopy] LaB6 Issues anyone?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Henk
Sorry, my experience is only with Tungsten but what about if you position
the cathode a little bit shifted to the opposite direction you suspect it
shifts after warming. So you may correct the problem and verify your theory
at the same time..
regards
yorgos


Dr Yorgos Nikas
Athens Innovative Microscopy
Skra 36 Voula 16673 GREECE

Tel/fax +30 210 8957677
mobile +30 6945 107477
www.eikonika.net www.aim.cat
*************************************



-----Original Message-----
X-from: colijn.1-at-osu.edu [mailto:colijn.1-at-osu.edu]
Sent: Thursday, April 21, 2016 3:56 AM
To: eikonika-at-otenet.gr

Hi John, et al,

I've had some issues over the past several years with the Kimball Physics
LaB6 cathodes. (I'm not sure which brand you are using.) After
meticulously setting the depth and centering the cathode, I run out of gun
tilt when warming it up. After pulling it back out of the scope, I find
that the cathode is still perfectly centered in the wehnelt. I often go
through 3 or 4 iterations before I can get a usable setting of the cathode,
each time finding that it looks perfectly centered outside of the scope.

My suspicion is that the legs have slightly different resistances and that
one leg warms up more than the other pushing the cathode to the side. When
the cathode cools back down, the tip recenters itself. This theory is
pretty hard to verify since I can't directly observe the tip while it is
warm. If I can get the tip centered enough I do see the "maltese cross"
figure though sometimes it's a bit off center.

One thing that may affect your lifetime is the filament limit for
saturation. I have noticed that if I initially set the cathode to be just
saturated, then check it 20-30 minutes later I find I can back the filament
drive off by 1 or 2 clicks and it is still saturated. I assume that as the
components warm up, the saturation point changes slightly. I do have to tell
the students that this is the correct way to operate so that they don't
reset the limit themselves. So, I now set my filament limit to be at least
one click undersaturated. This way, as it warms up, it will bring itself to
the proper saturation point. Note that the Kimball Physics documents
indicate that the evaporation rate will triple in going from 1800K to 1850K.
This will bring the lifetime from ~2000 hours down to a 600-700 hour range.

If the tip isn't oxidized by one of the students, I can still get close to
2000 hours from a cathode. I did have one student destroy one in 20 minutes
by letting the cold trap warm up while it was hot. (I was NOT
happy.)
Kimball Physics has some useful info in the technical documents on their web
site.

Cheers,
Henk

--------------------

Hendrik O. Colijn
Center for Electron Microscopy and AnalysiS The Ohio State University
1305 Kinnear Rd, Suite 100, Columbus, OH 43212

colijn.1-at-osu.edu 614/643-3458
cemas.osu.edu {about:cemas.osu.edu}

"Time is that quality of nature which keeps things from happening all at
once." (Ray Cummings - 1922)
Lately it doesn't seem to be working.



------ Original Message ------
X-from: oshel1pe-at-cmich.edu
To: colijn.1-at-osu.edu
Sent: 4/20/2016 4:01:46 PM

Note! Please do *not* use "Reply"! Send any response to the list or to
John L. Grazul {jlg98-at-cornell.edu} . I'm sending this for a colleague
locked behind a Cornell Curtain. Phil

Message:
Fellow microscopists it has been a long time since I have posted mainly
because anything that comes out of Cornell is un-postable for really
good reasons I assume. The huge issue I have been having for the past
couple of years is the quality, reliability and life span of my LaB6
sources. Now this is not an issue with just one scope or just one
vendor. Tungsten filaments work fantastic and hit all the numbers
perfectly in both of our 120kV Tecnais. When we put a LaB6 in really
weird things happen. Before you get suspicious and think we just
started
up with LaB6, this is all that we have ever used on our tools, I just
had to test the scopes with W to make sure I wasn?t imagining things.
We
need LaB6 because of the brightness and hours of use; each scope is
used
40-50 hr/week. So much for the introduction, now on to the issues:

1. I have not seen a Maltese cross on either a Kimball or Denka in 5
years or so, or about 5- 8 filaments, on either of my Tecnais. The
engineers and I just undersaturate and go for max brightness.
2. Filament life used to be 2500-3000 hours, the last five tips have
flamed out around 500 hours. BTW the vacuum has been rock steady in
both
scopes.
3. I have had two tips fail due to micro cracks in the carbon crucible.
4. The last five tips drift for the first 100-200 hours, and we are
talking X-Y and Z. This is to the point that the deflectors can?t
compensate and I have to break vacuum and re-center manually.

5. My 24 Volt power supply for the filament is fine, swapped them
around
my scopes with the same result.

If the darn things didn?t cost nearly $900.00 I might tolerate this. I
have to give high marks to EMS, they have been listening to my
complaints and have made good on the tips that have failed out of the
box. But the quality of the product I really have questions about. Has
Kimball changed their carbon supplier, is it softer? Why are there
cracks? And the last question is have any of you guys had the same or
similar issues? And the absolutely final question is am I missing
something? After 30+ years of ripping scopes apart I might just have
lost the magic touch or there is a vortex of Lab6 instability issues in
Ithaca.

Please contact me on or off line:
John L. Grazul {jlg98-at-cornell.edu}
--

==============================Original
Headers==============================
6, 33 -- From oshel1pe-at-cmich.edu Wed Apr 20 14:58:44 2016
6, 33 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116])
6, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
u3KJwiex003213
6, 33 -- for {microscopy-at-microscopy.com} ; Wed, 20 Apr 2016 14:58:44
-0500
6, 33 -- Received: from cas.cmich.edu ([141.209.15.90])
6, 33 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id
u3KK0dwT018896
6, 33 -- for {microscopy-at-microscopy.com} ; Wed, 20 Apr 2016 16:00:39
-0400
6, 33 -- Received: from cas4.central.cmich.local
(2002:8dd1:f03::8dd1:f03) by
6, 33 -- CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) with



From: microscopy.listserver-at-gmail.com
Date: April 20, 2016 at 7:43:13 PM CDT
Subject: [Microscopy] LaB6 Issues anyone?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi John, et al,

I've had some issues over the past several years with the Kimball
Physics LaB6 cathodes. (I'm not sure which brand you are using.) After
meticulously setting the depth and centering the cathode, I run out of
gun tilt when warming it up. After pulling it back out of the scope, I
find that the cathode is still perfectly centered in the wehnelt. I
often go through 3 or 4 iterations before I can get a usable setting of
the cathode, each time finding that it looks perfectly centered outside
of the scope.

My suspicion is that the legs have slightly different resistances and
that one leg warms up more than the other pushing the cathode to the
side. When the cathode cools back down, the tip recenters itself. This
theory is pretty hard to verify since I can't directly observe the tip
while it is warm. If I can get the tip centered enough I do see the
"maltese cross" figure though sometimes it's a bit off center.

One thing that may affect your lifetime is the filament limit for
saturation. I have noticed that if I initially set the cathode to be
just saturated, then check it 20-30 minutes later I find I can back the
filament drive off by 1 or 2 clicks and it is still saturated. I assume
that as the components warm up, the saturation point changes slightly. I
do have to tell the students that this is the correct way to operate so
that they don't reset the limit themselves. So, I now set my filament
limit to be at least one click undersaturated. This way, as it warms
up, it will bring itself to the proper saturation point. Note that the
Kimball Physics documents indicate that the evaporation rate will triple
in going from 1800K to 1850K. This will bring the lifetime from ~2000
hours down to a 600-700 hour range.

If the tip isn't oxidized by one of the students, I can still get close
to 2000 hours from a cathode. I did have one student destroy one in 20
minutes by letting the cold trap warm up while it was hot. (I was NOT
happy.)
Kimball Physics has some useful info in the technical documents on their
web site.

Cheers,
Henk

--------------------

Hendrik O. Colijn
Center for Electron Microscopy and AnalysiS
The Ohio State University
1305 Kinnear Rd, Suite 100, Columbus, OH 43212

colijn.1-at-osu.edu 614/643-3458
cemas.osu.edu {about:cemas.osu.edu}

"Time is that quality of nature which keeps things from happening all at
once." (Ray Cummings - 1922)
Lately it doesn't seem to be working.



------ Original Message ------
X-from: oshel1pe-at-cmich.edu
To: colijn.1-at-osu.edu
Sent: 4/20/2016 4:01:46 PM

Note! Please do *not* use "Reply"! Send any response to the list or to
John L. Grazul {jlg98-at-cornell.edu} . I'm sending this for a colleague
locked behind a Cornell Curtain. Phil

Message:
Fellow microscopists it has been a long time since I have posted mainly
because anything that comes out of Cornell is un-postable for really
good reasons I assume. The huge issue I have been having for the past
couple of years is the quality, reliability and life span of my LaB6
sources. Now this is not an issue with just one scope or just one
vendor. Tungsten filaments work fantastic and hit all the numbers
perfectly in both of our 120kV Tecnais. When we put a LaB6 in really
weird things happen. Before you get suspicious and think we just
started
up with LaB6, this is all that we have ever used on our tools, I just
had to test the scopes with W to make sure I wasn?t imagining things.
We
need LaB6 because of the brightness and hours of use; each scope is
used
40-50 hr/week. So much for the introduction, now on to the issues:

1. I have not seen a Maltese cross on either a Kimball or Denka in 5
years or so, or about 5- 8 filaments, on either of my Tecnais. The
engineers and I just undersaturate and go for max brightness.
2. Filament life used to be 2500-3000 hours, the last five tips have
flamed out around 500 hours. BTW the vacuum has been rock steady in
both
scopes.
3. I have had two tips fail due to micro cracks in the carbon crucible.
4. The last five tips drift for the first 100-200 hours, and we are
talking X-Y and Z. This is to the point that the deflectors can?t
compensate and I have to break vacuum and re-center manually.

5. My 24 Volt power supply for the filament is fine, swapped them
around
my scopes with the same result.

If the darn things didn?t cost nearly $900.00 I might tolerate this. I
have to give high marks to EMS, they have been listening to my
complaints and have made good on the tips that have failed out of the
box. But the quality of the product I really have questions about. Has
Kimball changed their carbon supplier, is it softer? Why are there
cracks? And the last question is have any of you guys had the same or
similar issues? And the absolutely final question is am I missing
something? After 30+ years of ripping scopes apart I might just have
lost the magic touch or there is a vortex of Lab6 instability issues in
Ithaca.

Please contact me on or off line:
John L. Grazul {jlg98-at-cornell.edu}
--

==============================Original
Headers==============================
6, 33 -- From oshel1pe-at-cmich.edu Wed Apr 20 14:58:44 2016
6, 33 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116])
6, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
u3KJwiex003213
6, 33 -- for {microscopy-at-microscopy.com} ; Wed, 20 Apr 2016 14:58:44
-0500
6, 33 -- Received: from cas.cmich.edu ([141.209.15.90])
6, 33 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id
u3KK0dwT018896
6, 33 -- for {microscopy-at-microscopy.com} ; Wed, 20 Apr 2016 16:00:39
-0400
6, 33 -- Received: from cas4.central.cmich.local
(2002:8dd1:f03::8dd1:f03) by
6, 33 -- CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) with



From: microscopy.listserver-at-gmail.com
Date: April 20, 2016 at 3:37:21 PM CDT
Subject: [Microscopy] Re: LaB6 Issues anyone?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi John,

we've had similar issues with the LaB6 filament image (odd shape) and
used the gun tilt to max out the screen current as an alignment
technique. We also have the filament image moving across the screen
when saturation is being approached. But the latter is what I've been
told is due to some dirt on the final anode or some nearby electrode
perhaps in the accelerator. This wandering is happening with W
filaments too and is less critical at lower mags.

I went to put a new LaB6 filament in the other day and it just broke
off, much to my embarrassment and I'm putting off buying a new one for a
while. FYI our microscope is a JEOL 2000FX and vacuum isn't a problem
so long as we replace the ion pump every five years. The new one that
broke while I was putting it in was a KP (the Denka are welded and robust).

The thing that has my blood boiling is the so-called long-life FEI
gallium sources that don't last more than three months. And as you may
know they come in at over $2000 each, as do the expendable extractors
similarly priced. The one we got with the new install lasted very
nicely, but since then not so much.

Best of luck with your search here,
Rob Keyse



On 4/20/2016 4:16 PM, oshel1pe-at-cmich.edu wrote:
----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Note! Please do *not* use "Reply"! Send any response to the list or to
John L. Grazul {jlg98-at-cornell.edu} . I'm sending this for a colleague
locked behind a Cornell Curtain. Phil

Message:
Fellow microscopists it has been a long time since I have posted mainly
because anything that comes out of Cornell is un-postable for really
good reasons I assume. The huge issue I have been having for the past
couple of years is the quality, reliability and life span of my LaB6
sources. Now this is not an issue with just one scope or just one
vendor. Tungsten filaments work fantastic and hit all the numbers
perfectly in both of our 120kV Tecnais. When we put a LaB6 in really
weird things happen. Before you get suspicious and think we just started
up with LaB6, this is all that we have ever used on our tools, I just
had to test the scopes with W to make sure I wasnt imagining things. We
need LaB6 because of the brightness and hours of use; each scope is used
40-50 hr/week. So much for the introduction, now on to the issues:

1. I have not seen a Maltese cross on either a Kimball or Denka in 5
years or so, or about 5- 8 filaments, on either of my Tecnais. The
engineers and I just undersaturate and go for max brightness.
2. Filament life used to be 2500-3000 hours, the last five tips have
flamed out around 500 hours. BTW the vacuum has been rock steady in both
scopes.
3. I have had two tips fail due to micro cracks in the carbon crucible.
4. The last five tips drift for the first 100-200 hours, and we are
talking X-Y and Z. This is to the point that the deflectors cant
compensate and I have to break vacuum and re-center manually.

5. My 24 Volt power supply for the filament is fine, swapped them around
my scopes with the same result.

If the darn things didnt cost nearly $900.00 I might tolerate this. I
have to give high marks to EMS, they have been listening to my
complaints and have made good on the tips that have failed out of the
box. But the quality of the product I really have questions about. Has
Kimball changed their carbon supplier, is it softer? Why are there
cracks? And the last question is have any of you guys had the same or
similar issues? And the absolutely final question is am I missing
something? After 30+ years of ripping scopes apart I might just have
lost the magic touch or there is a vortex of Lab6 instability issues in
Ithaca.

Please contact me on or off line:
John L. Grazul {jlg98-at-cornell.edu}

--
Robert Keyse, DPhil
Research Scientist
Lehigh University

5, East Packer Avenue,
Whitaker Laboratory
Bethlehem,
PA 18015-3194

Office (610)758-3465
Fax (610)758-4244


==============================Original Headers==============================
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From: microscopy.listserver-at-gmail.com
Date: April 20, 2016 at 2:58:45 PM CDT
Subject: [Microscopy] LaB6 Issues anyone?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Note! Please do *not* use "Reply"! Send any response to the list or to
John L. Grazul {jlg98-at-cornell.edu} . I'm sending this for a colleague
locked behind a Cornell Curtain. Phil

Message:
Fellow microscopists it has been a long time since I have posted mainly
because anything that comes out of Cornell is un-postable for really
good reasons I assume. The huge issue I have been having for the past
couple of years is the quality, reliability and life span of my LaB6
sources. Now this is not an issue with just one scope or just one
vendor. Tungsten filaments work fantastic and hit all the numbers
perfectly in both of our 120kV Tecnais. When we put a LaB6 in really
weird things happen. Before you get suspicious and think we just started
up with LaB6, this is all that we have ever used on our tools, I just
had to test the scopes with W to make sure I wasnt imagining things. We
need LaB6 because of the brightness and hours of use; each scope is used
40-50 hr/week. So much for the introduction, now on to the issues:

1. I have not seen a Maltese cross on either a Kimball or Denka in 5
years or so, or about 5- 8 filaments, on either of my Tecnais. The
engineers and I just undersaturate and go for max brightness.
2. Filament life used to be 2500-3000 hours, the last five tips have
flamed out around 500 hours. BTW the vacuum has been rock steady in both
scopes.
3. I have had two tips fail due to micro cracks in the carbon crucible.
4. The last five tips drift for the first 100-200 hours, and we are
talking X-Y and Z. This is to the point that the deflectors cant
compensate and I have to break vacuum and re-center manually.

5. My 24 Volt power supply for the filament is fine, swapped them around
my scopes with the same result.

If the darn things didnt cost nearly $900.00 I might tolerate this. I
have to give high marks to EMS, they have been listening to my
complaints and have made good on the tips that have failed out of the
box. But the quality of the product I really have questions about. Has
Kimball changed their carbon supplier, is it softer? Why are there
cracks? And the last question is have any of you guys had the same or
similar issues? And the absolutely final question is am I missing
something? After 30+ years of ripping scopes apart I might just have
lost the magic touch or there is a vortex of Lab6 instability issues in
Ithaca.

Please contact me on or off line:
John L. Grazul {jlg98-at-cornell.edu}
--

==============================Original Headers==============================
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From: microscopy.listserver-at-gmail.com
Date: April 20, 2016 at 8:27:26 AM CDT
Subject: [Microscopy] Fwd: viaWWW:automatic tissue processor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

JoAnn,
In my previous job, our processing workhorse was the Lynx EM tissue
processor (the version with the white body and smoke plastic lid). We
were quite happy with it, with no problems beyond the occasional user
error. We would start with buffer, and proceed through osmium, rinses,
graded ETOH, propylene oxide, and then into 3 dilutions of resin. All
that was left for us the next day was embedding.

Good luck,
~Gregg

Gregg Sobocinski
Microscope Imaging Specialist
University of Michigan, MCDB Dept.
Ann Arbor, Michigan, USA

On Tue, Apr 19, 2016 at 6:56 PM, {microscopy.listserver-at-gmail.com} wrote:




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Email: buchsmith-at-gmail.com
Name: JoAnn Buchanan

Organization: Allen Institute for Brain Science

Title-Subject: [Filtered] automatic tissue processor

Message: Hello listers. I am interested in finding whether there is a processor out there that can
take EM samples through dehydration steps.

We will be preparing large tissue blocks (1mm cube) and they need long dehydration steps(30 min
each). The current protocol means running up tissue late into the night. A robot would be helpful.

thanks in advance.
JoAnn

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From: microscopy.listserver-at-gmail.com
Date: April 19, 2016 at 5:40:36 PM CDT
Subject: [Microscopy] viaWWW:automatic tissue processor

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Email: buchsmith-at-gmail.com
Name: JoAnn Buchanan

Organization: Allen Institute for Brain Science

Title-Subject: [Filtered] automatic tissue processor

Message: Hello listers. I am interested in finding whether there is a processor out there that can
take EM samples through dehydration steps.

We will be preparing large tissue blocks (1mm cube) and they need long dehydration steps(30 min
each). The current protocol means running up tissue late into the night. A robot would be helpful.

thanks in advance.
JoAnn

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From: microscopy.listserver-at-gmail.com
Date: April 19, 2016 at 5:39:50 PM CDT
Subject: [Microscopy] viaWWW:(Microscopy) observing a diamond knife in the SEM

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Name: Lita Duraine

Organization: Howard Hughes Medical Institute

Title-Subject: [Filtered] Re: (Microscopy) observing a diamond knife in the SEM

Message: Hi Stephane,
I'm not sure about putting the diamond knife in the SEM. The epoxy that holds the diamond in place
might be compromised. For sure you will have charging from the epoxy.

It might be better to look at the diamond edge under a higher mag in a light microscope. For sure
you have either a nick or some hard debris causing your problem. It happens to me a lot because we
section drosophila tissue. Try cleaning again with soapy water, rinsing, followed by ethanol. If
that doesn't work it might be time to move to the next area or get it resharpened.

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From: microscopy.listserver-at-gmail.com
Date: April 18, 2016 at 8:41:53 PM CDT
Subject: [Microscopy] Type of sputter coating needed for Field Emission SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

You may be interested in checking out the prices on this web site. I remember Pt far outpacing Au back some years. I suppose Ir was even pricier.
Now, Ir comes in at $525/troy ounce, Pt comes in at $981, and Au comes in at $1242 - the most expensive metal in the table.

I suppose that is a supply and demand thing and that many individuals have turned to investing in gold in recent years. Ir and Pt apparently don't rate.

https://apps.catalysts.basf.com/apps/eibprices/mp/

FWIW,
Warren Straszheim

-----Original Message-----
X-from: mike_toalson-at-tedpella.com [mailto:mike_toalson-at-tedpella.com]
Sent: Monday, April 18, 2016 6:02 PM
To: Straszheim, Warren E [BIOTC]

I would agree that a coater capable of laying down a fine layer of Iridium is the least complicated solution, albeit not the cheapest due to target cost. We install a lot of coaters with Iridium targets for this very purpose (Cressington 208HR).

Anyone that would like a copy of an internal study on coating resolutions, please email me directly. You can also have a look at this comparative
study:

A comparative study of thin coatings of Au/Pd, Pt and Cr produced by magnetron sputtering for FE-SEM
Journal of Microscopy, Vol 189, Pt 1, January 1998, pp 79-89.

Mike Toalson
Ted Pella, Inc.
PO Box 492477 . Redding, CA 96049
Tel: 530-243-2200 x205 . Cell: 530-356-5921 mike_toalson-at-tedpella.com . www.tedpella.com




-----Original Message-----
X-from: wesaia-at-iastate.edu [mailto:wesaia-at-iastate.edu]
Sent: Tuesday, April 12, 2016 1:50 PM
To: Mike Toalson

Yes, that is probably right Au/Pd is probably too coarse. We had used gold
and the grains were very evident at 50kx.
We bought an iridium sputter coater and the grains are small enough that I
have never seen them. We often use less than 5 nm of coating thickness.

Warren Straszheim
Materials Analysis and Research Lab
Iowa State University

-----Original Message-----
X-from: tbargar-at-unmc.edu [mailto:tbargar-at-unmc.edu]
Sent: Tuesday, April 12, 2016 2:44 PM
To: Straszheim, Warren E [BIOTC]

Dear Listers,

What type or quality of sputter coating is needed for samples that will be
examined in a Field Emission SEM? I vaguely recall that your typical
gold/palladium is too coarse, is this correct?

Tom Bargar
UNMC
Electron Microscopy Core Facility
986395 Nebraska Medical Center
Omaha, NE 68198-6395
402-559-7347
tbargar-at-unmc.edu


The information in this e-mail may be privileged and confidential, intended
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From: microscopy.listserver-at-gmail.com
Date: April 18, 2016 at 6:00:31 PM CDT
Subject: [Microscopy] Type of sputter coating needed for Field Emission SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I would agree that a coater capable of laying down a fine layer of Iridium
is the least complicated solution, albeit not the cheapest due to target
cost. We install a lot of coaters with Iridium targets for this very
purpose (Cressington 208HR).

Anyone that would like a copy of an internal study on coating resolutions,
please email me directly. You can also have a look at this comparative
study:

A comparative study of thin coatings of Au/Pd, Pt and Cr produced by
magnetron sputtering for FE-SEM
Journal of Microscopy, Vol 189, Pt 1, January 1998, pp 79-89.

Mike Toalson
Ted Pella, Inc.
PO Box 492477 Redding, CA 96049
Tel: 530-243-2200 x205 Cell: 530-356-5921
mike_toalson-at-tedpella.com www.tedpella.com




-----Original Message-----
X-from: wesaia-at-iastate.edu [mailto:wesaia-at-iastate.edu]
Sent: Tuesday, April 12, 2016 1:50 PM
To: Mike Toalson

Yes, that is probably right Au/Pd is probably too coarse. We had used gold
and the grains were very evident at 50kx.
We bought an iridium sputter coater and the grains are small enough that I
have never seen them. We often use less than 5 nm of coating thickness.

Warren Straszheim
Materials Analysis and Research Lab
Iowa State University

-----Original Message-----
X-from: tbargar-at-unmc.edu [mailto:tbargar-at-unmc.edu]
Sent: Tuesday, April 12, 2016 2:44 PM
To: Straszheim, Warren E [BIOTC]

Dear Listers,

What type or quality of sputter coating is needed for samples that will be
examined in a Field Emission SEM? I vaguely recall that your typical
gold/palladium is too coarse, is this correct?

Tom Bargar
UNMC
Electron Microscopy Core Facility
986395 Nebraska Medical Center
Omaha, NE 68198-6395
402-559-7347
tbargar-at-unmc.edu


The information in this e-mail may be privileged and confidential, intended
only for the use of the addressee(s) above. Any unauthorized use or
disclosure of this information is prohibited. If you have received this
e-mail by mistake, please delete it and immediately contact the sender.



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From: microscopy.listserver-at-gmail.com
Date: April 18, 2016 at 11:58:36 AM CDT
Subject: [Microscopy] observing a diamond knife in SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Stephane,

I have a 3view, which is basically an ultra-microtome inside an SEM. I don't have conclusive answers, but I can give you my best opinion based on my experience.
The short answer is to image the resin rather than the knife in order to see the damage being caused. If you can cut a relatively wide bit of resin then you can coat the block and mount this in the SEM and image the block face. You may see lots of chatter there if you make the face very wide (chatter will look a bit like waves on the block face), but knife marks will come out like perfect straight lines. You'll also see if any seem particularly bad and changes in the amount and type of chatter will give you some information on whether the knife has the same sharpness across the blade. I'd use some empty resin for this sort of test so that you don't have sample density confusing the cutting properties.

To answer the other questions. I do believe that you would crack carbon deposits (particles of resin and, more likely, volatile resin components evaporated during imaging) and stick these onto the knife surface. Whether there would be enough to ruin the knife is another matter as they're likely to be tiny, but its probably safer to avoid imaging the blade directly.
Also diamond isn't electrically conductive as far as I know, so you would need to image in a low vacuum mode, or to coat the knife, but I'm not sure if the re-sharpener would like this.

Hope that this helps.

TobyS

Dr Tobias Starborg
Senior Experimental Officer: 3DEM
Wellcome Centre for Cell Matrix Research
Michael Smith Building
Manchester
M13 9PT
Tel: +44(0)1612755170
http://www.polara.manchester.ac.uk

________________________________________
X-from: nizets2-at-yahoo.com [nizets2-at-yahoo.com]
Sent: 18 April 2016 16:13
To: Tobias Starborg

Hello community!

I have a question about a microtome diamond knife and would be grateful if you would care to share your thoughts with me.
The diamond knife (Ultraknife) seems to be damaged or dirty in the middle so that one section is cut in half in the middle. Usually when the blade is damaged I can see some indentation under the binocular at highest mag but in this case I can see nothing, so I tried to clean the blade but it changes nothing.
Since we have a SEM, I planned to take a look of the blade in SEM but I wondered if, for any reason, I should avoid that.
- Is there a chance that I burn any dirt onto the blade with the electron beam? (I only cut epoxy so it would be some epoxy rests)
- Is there a chance that I burn some carbohydrates onto the blade in the same way it occurs in the TEM?
- What about charging? I am quite sure that there should be some contact between the diamond blade and the metal boat, so this does not worry me much.
- Any other thought? (Apart from the fact that I should avoid to hit the column with the blade :-))
Thanks in advance.

Regards,
Stephane


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From: microscopy.listserver-at-gmail.com
Date: April 18, 2016 at 9:55:00 AM CDT
Subject: [Microscopy] observing a diamond knife in SEM

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Hello community!

I have a question about a microtome diamond knife and would be grateful if you would care to share your thoughts with me.
The diamond knife (Ultraknife) seems to be damaged or dirty in the middle so that one section is cut in half in the middle. Usually when the blade is damaged I can see some "indentation" under the binocular at highest mag but in this case I can see nothing, so I tried to clean the blade but it changes nothing.
Since we have a SEM, I planned to take a look of the blade in SEM but I wondered if, for any reason, I should avoid that.
- Is there a chance that I "burn" any dirt onto the blade with the electron beam? (I only cut epoxy so it would be some epoxy rests)
- Is there a chance that I "burn" some carbohydrates onto the blade in the same way it occurs in the TEM?
- What about charging? I am quite sure that there should be some contact between the diamond blade and the metal boat, so this does not worry me much.
- Any other thought? (Apart from the fact that I should avoid to hit the column with the blade :-))
Thanks in advance.

Regards,
Stephane


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From: microscopy.listserver-at-gmail.com
Date: April 15, 2016 at 9:15:49 PM CDT
Subject: [Microscopy] viaWWW: SEM Advice for EDS of SS

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Normally, stainless steel should be a fairly straightforward analysis. I would recommend 15-20 kV so you could use the K lines for analysis. Current depends on your EDS settings. You would like to have a decent count rate to shorten the time it takes to get a good spectrum. I like to keep the dead time near but below 30%. What count rate that corresponds to depends on your x-ray detector and your pulse processor settings. On our Oxford X-Max detector, that is about 20 kcps at process time 4. About 0.5 nA gives us that.

I would cautiously let the INCA do auto ID and then confirm the elements. I would expect Fe, Cr, Ni, and maybe some Mn and Si. It depends on the alloy.

If it is possible to remove the piece from the non-conductive tape and transfer it to some carbon tape, I would do that. Hopefully there is not a lot of goo stuck to the chip. If so, you might have to work around it.

EDS in variable pressure mode opens you up to stray signals from the surroundings. The incoming electrons will get scattered and give you a noticeable signal. You would like to be sure that doesn't include any elements that you expect to be present in your sample. You shouldn't be attempting quant on C, so maybe the background holder can be painted with carbon paint. You might try analyses closer to the surroundings and further away to see how the composition changes. This means you will also have trouble trying to get good analyses from adjoining phases if multiple phases are present. You will be able to see which ones are higher and lower in elements, but the absolute levels will be hard to determine.

Also watch out for topography/geometry. EDS normally assumes a flat surface perpendicular to the beam. Tilted surfaces will enhance or detract from the light(er) elements. Watch the background shape and see how it compares to a flat sample known to be stainless steel. The good news for you is that the elements Cr-Ni are of most concern and they are relatively close to each other in energy and of moderate energy that is less subject to changes in absorption. Si is much lighter and more subject to changes.

Warren Straszheim
Materials Analysis and Research Lab
Iowa State University

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Name: Cynthia Ross Friedman

Organization: Thompson Rivers University

Title-Subject: [Filtered] Advice for EDS

Message: Hello -- I admit I am not very skilled with my Oxford Inca EDS yet. Someone has brought me
a metal artifact, and would like me to determine if it is stainless steel, and its purity.

Could someone please suggest to me the best parameters to use? Spot size? (in amps) Voltage? Other
considertations?

As an added complication, the sample was provided to me on a non-conductive tape, and to get around
the charging, I have to use my SEM (a Zeiss LS Evo) at about 55 Pa. I cannot see this being an
issue, but do let me know your thoughts.

Thanks in advance!
Cindy

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From: microscopy.listserver-at-gmail.com
Date: April 15, 2016 at 6:02:23 PM CDT
Subject: [Microscopy] viaWWW: SEM Advice for EDS of SS

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Title-Subject: [Filtered] Advice for EDS

Message: Hello -- I admit I am not very skilled with my Oxford Inca EDS yet. Someone has brought me
a metal artifact, and would like me to determine if it is stainless steel, and its purity.

Could someone please suggest to me the best parameters to use? Spot size? (in amps) Voltage? Other
considertations?

As an added complication, the sample was provided to me on a non-conductive tape, and to get around
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Thanks in advance!
Cindy

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From: microscopy.listserver-at-gmail.com
Date: April 15, 2016 at 6:01:11 PM CDT
Subject: [Microscopy] viaWWW: LM: autofocus systems

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Title-Subject: [Filtered] autofocus systems

Message: Hello everyone,

We are looking to upgrade one of our spinning disk systems with a new microscope and autofocus
system and are looking for comments/reviews of the latest versions from Leica, Zeiss, Nikon and Olympus.

We are familiar with how they function but are looking for comments on how well each works for long
term imaging.

Any advice would be greatly appreciated.

Garnet


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From: microscopy.listserver-at-gmail.com
Date: April 15, 2016 at 6:00:02 PM CDT
Subject: [Microscopy] viaWWW:Life sciences TEM sample prep short courses?

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Email: james.weston-at-nyu.edu
Name: James Weston

Organization: NYU Abu Dhabi

Title-Subject: [Filtered] Life sciences TEM sample prep short courses?

Message: Hello,
I am a materials engineer and am soon going to be in charge of a TEM for
both materials and biological work. I have no experience in life sciences
EM. The TEM manufacturer is providing off-site life science training,
however this will not cover sample preparation.

Is there a resource that has listings of sample prep short courses?

Any help/suggestions greatly appreciated.
James


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From: :      John.Mardinly-at-asu.edu
Date: 14 Apr 2016 06:46
Subject: [Microscopy] Re: Type of sputter coating needed for Field Emission

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Jisheng,
The systems we used at Intel were the VCR IBS, and later, the Gatan PECS (still available). These systems use an argon ion beam striking a target of your choice, and the material sputtered from the target lands on the specimen. The resulting film has an extremely fine grain size. I believe there was a description in one publication of Ron Anderson at IBM using a Gatan duo-mill to do ion beam deposition by putting the target where the TEM specimen should be, and putting the actual specimen ~1 cm away with a custom made fixture.

John Mardinly, Retired from ASU






On Apr 13, 2016, at 10:49 PM, Ji Sheng Ma {jisheng.ma-at-monash.edu} wrote:

Dear John,

I've got your email from the emal-list. I am very interested what you mentioned that "to get extremely fine grain size is to use ion-beam deposition".

I am wondering the ion-beam deposition is referring focused ion beam? If yes, it could be extremely useful. I thought (and I also tried) it was very hard to achieve very thin coating (usually the Pt coating with a lot of carbon) in a FIB microscope even use electron beam coating at a very low coating rate.

Maybe I misunderstood you. Otherwise please let me know if you think it is also possible to achieve good coating using FIB in a FIB microscope and your suggestion on coating procedures to do this.

Thanks for your time.

Kind Regards,

Jisheng


On 14 April 2016 at 15:22, Jisheng Ma {jisheng.ma-at-gmail.com} wrote:
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My experience with chromium is that it seems to oxidize during deposition, such that freshly deposited films that are covered with a cap that blocks exposure to air are up to half oxygen when analyzed. Nevertheless, under HRTEM, they appear to have grain size so much smaller than any TEM specimen thickness that the grains are not visible. Another way to get extremely fine grain size is to use ion-beam deposition rather than sputtering or evaporation.
Osmium, whatever it's merits from a grain size consideration, is hazardous, so you would need to implement a safety program around it, which could make it more trouble than it is worth.

John Mardinly, Retired from ASU

Hi Tom

I would guess that you are going to be working at a resolution that will exceed the grain size of Au/Pd. While there are certainly exceptions, I typically suggest the following as a very rough guideline:

Gold up to ~10kx mag
Gold/Palladium up to ~50kx mag
Platinum up to ~100-150kx mag
Above 150kx mag, either Chromium, Iridium, or Osmium. Each has its own advantages and drawbacks:

Chromium is probably the cheapest option, but oxidizes very quickly into a non-conductive film.
Iridium targets can be very expensive, but are available for most coaters.
Osmium coating is done in a dedicated coater that will not sputter other materials, but the film is inert and amorphous. The source material is relatively cheap, but the coaters are an investment.

A really good source of information is "Handbook of Sample Preparation for Scanning Electron Microscopy and X-Ray Microanalysis" by Patrick Echlin, who used to teach at the Lehigh Microscopy School.

I'd be happy to continue this offline if you have more questions, or are looking for more detailed information.

Best regards,

Jeff

Jeffrey A. Hall | Microscopist
SPI Supplies | 206 Garfield Ave. | West Chester, PA 19380, USA
1-610-436-5400 x106 | jhall-at-2spi.com | http://www.2spi.com
Twitter: http://2spi.com/twitter | Facebook: http://2spi.com/facebook


--
Dr. Jisheng Ma
FIB/SEM Microscopist and Ultramicrotomist

Monash Centre for Electron Microscopy
Monash University
Room G29, Building 81, Clayton Campus
10 Innovation Walk
Clayton VIC 3800
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From: microscopy.listserver-at-gmail.com
Date: April 14, 2016 at 6:33:05 AM CDT
Subject: [Microscopy] viaWWW:Removing exudates from insect wings

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Email: blayjorge-at-gmail.com
Name: Jorge A Santiago-Blay

Organization: NMNH

Title-Subject: [Filtered] Removing exudates from insect wings

Message: We have imaged beautiful structures on the surface of insect wings. Yet, we want to try to
eliminate the possibility some of the structures" are *not* some sort of exudate (e.g. cuticular
hydrocarbon, etc.) from the wings. I am aware that pentane, hexane can get the job done if the
materials are suspect to be thin layers but what is the stuff is thinker? Would hexamethyldisilane
(HMDS, in a hood) work?

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From: microscopy.listserver-at-gmail.com
Date: April 14, 2016 at 3:25:56 AM CDT
Subject: [Microscopy] Re: LEICA / Reichert Ultra-Trim manual - solved

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Dear All,
thanks, I got a copy.

Best,
Stefan

-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
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++49-175-7177051 Mobile

Websites:
www.nanoflight.info
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www.assisi.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
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Am 13.04.16 um 15:32 schrieb stefan.diller-at-t-online.de:
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Dear All,
anybody out there who can send me a PDF copy of the user manual of the Ultra-Trim?

Thanks,
Stefan



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From: microscopy.listserver-at-gmail.com
Date: April 14, 2016 at 2:44:17 AM CDT
Subject: [Microscopy] Re: Type of sputter coating needed for Field Emission

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Hi

Do you really need a coater ?

As we bought our first FE-SEM, we asked ourself the same question, and
finally bought... nothing. Our FE-SEM is now more then 15 years old, and
in 95% of the cases, playing with accellerating voltage, beam current,
detectors and scan speed, it's not necessary to coat. Our new FE-SEM is
now not far from the door, but without any coater again !

In the mean time I build a multi purpouse evaporator fitted among others
with a magnetron source. I bought then a Ir target, to be able to try
and in case it's necessary...
I use it from time to time for fabric samples, because I'm too lazy to
follow the drifting of the low energy condtions for these samples. But
they need only low mag and could be observed on a good W type SEM with
the classic 20 nm grain size gold coating from our 30 years old Balzers.

The only coating which remains necessary on FE-SEM is carbon for EDX.

Of coarse, as we work in material science (but often on oxydes) and not
in biology, our samples are probably different from yours.

2 cts !

Jacques

Le 12/04/2016 22:02, tbargar-at-unmc.edu a ecrit :
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Dear Listers,

What type or quality of sputter coating is needed for samples that will be examined in a Field Emission SEM? I vaguely recall that your typical gold/palladium is too coarse, is this correct?

Tom Bargar
UNMC
Electron Microscopy Core Facility
986395 Nebraska Medical Center
Omaha, NE 68198-6395
402-559-7347
tbargar-at-unmc.edu


The information in this e-mail may be privileged and confidential, intended only for the use of the addressee(s) above. Any unauthorized use or disclosure of this information is prohibited. If you have received this e-mail by mistake, please delete it and immediately contact the sender.



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From: microscopy.listserver-at-gmail.com
Date: April 13, 2016 at 8:11:02 PM CDT
Subject: [Microscopy] viaWWW:Electron Microscopy (EM) Workshop and Maryland NanoDay 2016

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Organization: TESCAN USA

Title-Subject: [Filtered] Electron Microscopy (EM) Workshop and Maryland NanoDay 2016 Conference

Message: Dear EM and FIB Friends:

I would like to take this opportunity to invite you to the Electron Microscopy (EM) Workshop and
Maryland NanoDay 2016 Conference that will be held at the University of Maryland, College Park,
Maryland on May 17 and 18, 2016.

Below are some of the highlights for this meeting:

* The theme of EM Workshop: Recent Advances in Electron and Ion Microscopy (EM Workshop Day, May
17). The EM Workshop covers a broad spectrum of newest development in electron microscopy and focus
ion beam and related technology.

* Plenary talks on nanoscience and nanotechnology (NanoDay, May 18).

* Platform presentations and poster session.

* Demos (including live demos both with lecture and also in the laboratory)

* Vendor exhibits (10~12 major companies)

* Free registration and meals.

Additional information and registration can be found at http://www.nanocenter.umd.edu

Best regards,

The AIM Lab
(Advamced Imaging and Microscopy Laboratory)
NanoCenter
University of Maryland
(301)-405-0541


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From: microscopy.listserver-at-gmail.com
Date: April 13, 2016 at 3:21:36 PM CDT
Subject: [Microscopy] Type of sputter coating needed for Field Emission SEM

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My experience with chromium is that it seems to oxidize during deposition, such that freshly deposited films that are covered with a cap that blocks exposure to air are up to half oxygen when analyzed. Nevertheless, under HRTEM, they appear to have grain size so much smaller than any TEM specimen thickness that the grains are not visible. Another way to get extremely fine grain size is to use ion-beam deposition rather than sputtering or evaporation.
Osmium, whatever it's merits from a grain size consideration, is hazardous, so you would need to implement a safety program around it, which could make it more trouble than it is worth.

John Mardinly, Retired from ASU

Hi Tom

I would guess that you are going to be working at a resolution that will exceed the grain size of Au/Pd. While there are certainly exceptions, I typically suggest the following as a very rough guideline:

Gold up to ~10kx mag
Gold/Palladium up to ~50kx mag
Platinum up to ~100-150kx mag
Above 150kx mag, either Chromium, Iridium, or Osmium. Each has its own advantages and drawbacks:

Chromium is probably the cheapest option, but oxidizes very quickly into a non-conductive film.
Iridium targets can be very expensive, but are available for most coaters.
Osmium coating is done in a dedicated coater that will not sputter other materials, but the film is inert and amorphous. The source material is relatively cheap, but the coaters are an investment.

A really good source of information is "Handbook of Sample Preparation for Scanning Electron Microscopy and X-Ray Microanalysis" by Patrick Echlin, who used to teach at the Lehigh Microscopy School.

I'd be happy to continue this offline if you have more questions, or are looking for more detailed information.

Best regards,

Jeff

Jeffrey A. Hall | Microscopist
SPI Supplies | 206 Garfield Ave. | West Chester, PA 19380, USA
1-610-436-5400 x106 | jhall-at-2spi.com | http://www.2spi.com
Twitter: http://2spi.com/twitter | Facebook: http://2spi.com/facebook



-----Original Message-----
X-from: tbargar-at-unmc.edu [mailto:tbargar-at-unmc.edu]
Sent: Tuesday, April 12, 2016 4:01 PM
To: Jeff Hall {jhall-at-2spi.com}

Dear Listers,

What type or quality of sputter coating is needed for samples that will be examined in a Field Emission SEM? I vaguely recall that your typical gold/palladium is too coarse, is this correct?

Tom Bargar
UNMC
Electron Microscopy Core Facility
986395 Nebraska Medical Center
Omaha, NE 68198-6395
402-559-7347
tbargar-at-unmc.edu


The information in this e-mail may be privileged and confidential, intended only for the use of the addressee(s) above. Any unauthorized use or disclosure of this information is prohibited. If you have received this e-mail by mistake, please delete it and immediately contact the sender.



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From: microscopy.listserver-at-gmail.com
Date: April 13, 2016 at 1:25:51 PM CDT
Subject: [Microscopy] LM cryomicrotome: need new thick section unit that cuts at -150

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Hello,

We need a new cryomicrotome for 'thick' sectioning for LM transmission imaging.
We cut rubber and plastic "thick" sections (c.1 micron thick).
We now use a Leica RM2165 cryomicrotome with the LN21 cryo unit to do this.
The LN21 cools the specimen to -150 degrees C.
Our equipment is getting old and we need a replacement very soon.
The RM2265 can still be purchased from Leica.
However, apparently, the LN22 cryo unit that fits the RM2265 has been
discontinued.
As for cryostats: the minimum temperatures are too high (typically -50
degrees C).
Therefore, we cannot use a typical cryostat (such as for biological tissue etc.)
We have searched the internet and been unsuccessful in finding information.

Can anyone direct us to a solution 'similar' to the RM2265/LN22 from
any USA supplier?
We prefer new equipment as opposed to used.
Thanks in advance for your advice.

Tyler Gruber
tylerdotgruberatadityabirladotcom

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From: microscopy.listserver-at-gmail.com
Date: April 13, 2016 at 8:25:31 AM CDT
Subject: [Microscopy] LEICA / Reichert Ultra-Trim manual

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Dear All,
anybody out there who can send me a PDF copy of the user manual of the Ultra-Trim?

Thanks,
Stefan

--


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Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
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www.assisi.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
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From: microscopy.listserver-at-gmail.com
Date: April 13, 2016 at 7:59:31 AM CDT
Subject: [Microscopy] viaWWW:RMS Medal Series Nominations

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Email: mel-at-rms.org.uk
Name: Mel Reedman

Organization: Royal Microscopical Society

Title-Subject: [Filtered] RMS Medal Series Nominations

Message: Royal Microscopical Society Medal Series - Nominations Invited
www.rms.org.uk/medal-series

The RMS Medal Series, awards scientific achievements in each of the Science Sections as well as
awarding the unsung heroes and those who volunteer a huge amount of their time and energy to the
RMS, helping the next generation of microscopists.

The RMS are inviting nominations for the following Medals until 30 April 2016.

- President's Medal for Services to the Society for an exceptional voluntary contribution to the
work of the RMS.
- Vice-Presidents' Medal for Microscopy Research and Laboratory Support recognises the unsung
heroes of microscopy by making an award to an engineer, technician or laboratory research support
scientist.
- Alan Agar Medal for Electron Microscopy outstanding scientific achievements applying electron
microscopy in the field of physical or life sciences.
- Medal for Light Microscopy outstanding scientific achievements applying or developing new forms
of light microscopy.
- Medal for Life Sciences outstanding scientific achievements applying microscopy in the field of
cell biology.
- Medal for Innovation in Applied Microscopy for Materials Science outstanding scientific
achievements in applying microscopy in the field of materials science.
- Medal for Scanning Probe Microscopy outstanding progress made in the field of scanning probe
microscopy (SPM.)
- The Pearse Prize a scientist who has made a valuable contribution either to the development of a
new technique or through the application of existing methods.

Find out more and make your nomination at www.rms.org.uk/medal-series


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From: microscopy.listserver-at-gmail.com
Date: April 12, 2016 at 3:45:29 PM CDT
Subject: [Microscopy] Type of sputter coating needed for Field Emission SEM

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Hello Tom,
It depends mainly on your samples and resolution you want to get. However, for FEG you should use a high resolution sputter coater. I have good experiences with platinum coating (~ 3 nm) for biological samples.
For x-ray microanalysis we use 10 nm of silver coating (biological samples). In some cases we do not coat the samples at all.
But all depends on detectors you have on your FEG and the mode of FEG SEM you use.

Best regards

Oldrich

--
Oldich Benada
Institute of Microbiology CAS, v.v.i.
Laboratory of Molecular Structure Characterization
Videska 1083
142 20 Prague 4
Czech Republic

On Tue, 12 Apr 2016 14:47:04 -0500, tbargar-at-unmc.edu wrote :



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Dear Listers,

What type or quality of sputter coating is needed for samples that
will be examined in a Field Emission SEM? I vaguely recall that your
typical gold/palladium is too coarse, is this correct?

Tom Bargar
UNMC
Electron Microscopy Core Facility
986395 Nebraska Medical Center
Omaha, NE 68198-6395
402-559-7347
tbargar-at-unmc.edu


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contact the sender.



==============================Original
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14.03.0235.001; Tue, 12 Apr 2016 14:43:38 -0500 7, 37 -- From:
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Hi Tom

I would guess that you are going to be working at a resolution that will exceed the grain size of Au/Pd. While there are certainly exceptions, I typically suggest the following as a very rough guideline:

Gold up to ~10kx mag
Gold/Palladium up to ~50kx mag
Platinum up to ~100-150kx mag
Above 150kx mag, either Chromium, Iridium, or Osmium. Each has its own advantages and drawbacks:

Chromium is probably the cheapest option, but oxidizes very quickly into a non-conductive film.
Iridium targets can be very expensive, but are available for most coaters.
Osmium coating is done in a dedicated coater that will not sputter other materials, but the film is inert and amorphous. The source material is relatively cheap, but the coaters are an investment.

A really good source of information is "Handbook of Sample Preparation for Scanning Electron Microscopy and X-Ray Microanalysis" by Patrick Echlin, who used to teach at the Lehigh Microscopy School.

I'd be happy to continue this offline if you have more questions, or are looking for more detailed information.

Best regards,

Jeff

Jeffrey A. Hall | Microscopist
SPI Supplies | 206 Garfield Ave. | West Chester, PA 19380, USA
1-610-436-5400 x106 | jhall-at-2spi.com | http://www.2spi.com
Twitter: http://2spi.com/twitter | Facebook: http://2spi.com/facebook



-----Original Message-----
X-from: tbargar-at-unmc.edu [mailto:tbargar-at-unmc.edu]
Sent: Tuesday, April 12, 2016 4:01 PM
To: Jeff Hall {jhall-at-2spi.com}

Dear Listers,

What type or quality of sputter coating is needed for samples that will be examined in a Field Emission SEM? I vaguely recall that your typical gold/palladium is too coarse, is this correct?

Tom Bargar
UNMC
Electron Microscopy Core Facility
986395 Nebraska Medical Center
Omaha, NE 68198-6395
402-559-7347
tbargar-at-unmc.edu


The information in this e-mail may be privileged and confidential, intended only for the use of the addressee(s) above. Any unauthorized use or disclosure of this information is prohibited. If you have received this e-mail by mistake, please delete it and immediately contact the sender.



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From: microscopy.listserver-at-gmail.com
Date: April 12, 2016 at 3:29:42 PM CDT
Subject: [Microscopy] Type of sputter coating needed for Field Emission SEM

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Yes, that is probably right Au/Pd is probably too coarse. We had used gold and the grains were very evident at 50kx.
We bought an iridium sputter coater and the grains are small enough that I have never seen them. We often use less than 5 nm of coating thickness.

Warren Straszheim
Materials Analysis and Research Lab
Iowa State University

-----Original Message-----
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Sent: Tuesday, April 12, 2016 2:44 PM
To: Straszheim, Warren E [BIOTC]

Dear Listers,

What type or quality of sputter coating is needed for samples that will be examined in a Field Emission SEM? I vaguely recall that your typical gold/palladium is too coarse, is this correct?

Tom Bargar
UNMC
Electron Microscopy Core Facility
986395 Nebraska Medical Center
Omaha, NE 68198-6395
402-559-7347
tbargar-at-unmc.edu


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From: microscopy.listserver-at-gmail.com
Date: April 12, 2016 at 3:17:04 PM CDT
Subject: [Microscopy] Microwave processor trouble

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This is a shot in the dark.

I have a microwave oven tissue processor. I can't make it work.

Symptoms:

Key pad for entering times is dead most of the time. Sometimes it works, mostly not. It's one of those flat, membrane type pads.

Can't get anything to heat up in the chamber. No action with neon light array either.

I know about temp restriction, that's not it.

Don't know much about these things, like how to fix them or if they are fixable.

Any microwave gurus available for consultation?

Jon

Jonathan Krupp
ASB&T
San Joaquin Delta College
5151Pacific Ave.
Stockton, CA 95207
(209) 954-5284
jkrupp-at-deltacollege.edu



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From: microscopy.listserver-at-gmail.com
Date: April 12, 2016 at 2:42:13 PM CDT
Subject: [Microscopy] Type of sputter coating needed for Field Emission SEM

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Dear Listers,

What type or quality of sputter coating is needed for samples that will be examined in a Field Emission SEM? I vaguely recall that your typical gold/palladium is too coarse, is this correct?

Tom Bargar
UNMC
Electron Microscopy Core Facility
986395 Nebraska Medical Center
Omaha, NE 68198-6395
402-559-7347
tbargar-at-unmc.edu


The information in this e-mail may be privileged and confidential, intended only for the use of the addressee(s) above. Any unauthorized use or disclosure of this information is prohibited. If you have received this e-mail by mistake, please delete it and immediately contact the sender.



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From: microscopy.listserver-at-gmail.com
Date: April 11, 2016 at 10:38:48 AM CDT
Subject: [Microscopy] Z6040 with HM20

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Hello All,


I have a need to add the Z6040 to HM20 resin to be cured with UV -at- -45C.
Ive been told that Z6040 can be used with HM20 however, I did a test
block and the resin never cured.

Has anyone used Z6040 with HM20? Did you have to change the amount of
accelerator?


I use Z6040 Primer (EMS-50440-10) with Epoxy resins and LRW heat and cold
cured. The primer always works with these resins and I havent needed to
change the recipe.

Karen



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From: microscopy.listserver-at-gmail.com
Date: April 8, 2016 at 6:37:17 AM CDT
Subject: [Microscopy] viaWWW:CPD system suggestions

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Name: Dr. Gary Gaugler

Organization: Microtechnics

Title-Subject: [Filtered] CPD system suggestions

Message: Greetings listers. I'm seeking feedback about CPD units for dehydration of bio specimens
for SEM. I'd like an automated system that does more push button GO than manual knob manipulation.
Any suggestions for this type of unit? For CO2, what general size cylinders are useful for
occasional drying?

All inputs are appreciated, TIA.
gary g.


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From: microscopy.listserver-at-gmail.com
Date: April 7, 2016 at 10:10:07 PM CDT
Subject: [Microscopy] Re: viaWWW:TEM Florescent Screen Cleaning

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Usually the screens get covered, more toward the center, with carbon contamination, which makes them darker. Occasionally they will get tiny burns by someone doing microbeam analysis or diffraction. There a few companies that re-coat them (for a MUCH lower price than the OEM manufacturer) that can be found by searching "fluorescent screen re-coating" in Google. Even Amazon.com.uk lists a service!

John Mardinly, Retired from ASU





On Apr 7, 2016, at 5:18 PM, microscopy.listserver-at-gmail.com wrote:




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Email: mj_iqbal-at-yahoo.com
Name: M javed Iqbal

Organization: National Institute for Biotechnology and Genetic Engineering NIBGE Pakistan

Title-Subject: [Filtered] TEM Florescent Screen Cleaning

Message: I would like to hear from microscopy listserver regarding the transmission electron
microscope florescent screen service which has some dirt particles accumulated over a period of
years likely to be the traces of carbon film /sample charging effect of nano materials and etc.
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From: microscopy.listserver-at-gmail.com
Date: April 7, 2016 at 7:04:16 PM CDT
Subject: [Microscopy] viaWWW:KMS310 COMPLETE KIT

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


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Email: jeyoucor-at-chol.com
Name: Mr. Michael Kim

Organization: B&F CORPORATION

Title-Subject: [Filtered] KMS310 COMPLETE KIT

Message: I would like to buy the used KMS310 or KMS400 from ZYGO.
But now, there are possible tools to buy WITHOUT the ELECTRONIC CONTROL TOWER UNIT which contains
the O/S,software and some modules inside only.

If anybody has solution for this pending matter, pls advise.

Thanks,

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From: microscopy.listserver-at-gmail.com
Date: April 7, 2016 at 6:59:57 PM CDT
Subject: [Microscopy] viaWWW:TEM Florescent Screen Cleaning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

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Email: mj_iqbal-at-yahoo.com
Name: M javed Iqbal

Organization: National Institute for Biotechnology and Genetic Engineering NIBGE Pakistan

Title-Subject: [Filtered] TEM Florescent Screen Cleaning

Message: I would like to hear from microscopy listserver regarding the transmission electron
microscope florescent screen service which has some dirt particles accumulated over a period of
years likely to be the traces of carbon film /sample charging effect of nano materials and etc.
Thanks in advanec

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From: microscopy.listserver-at-gmail.com
Date: April 7, 2016 at 12:56:32 PM CDT
Subject: [Microscopy] Philadelphia Society For Microscopy Meeting - Thursday April 21st Villanova University

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

PSM's April meeting will be held on Thursday the 21st of April at Villanova
University at 6pm. There will be a short business meeting (with pizza)
followed by a talk by Linden Gledhill.

Here is some information on Linden and the talk. The presentation "Creating
Commercial Art from Microscopy" will focus on the creative process and
provide insight through discussion of specific projects for commercial
marketing. Linden Gledhill is an artist who explores the physical world at
different image scales and fragments of time. His education in science has
led to the use of advanced microscopy and high speed equipment to create
unexpected imagery revealing the physical beauty which surrounds us.

A passion for photography has led to numerous collaborations with other
artists and designers resulting in promotional art work and effective
advertisement campaigns. His work can be found in a wide range of products
including books, snow boards, record sleeves, promotional video and apparel.
Corporate clients included ExxonMobil, GMC, Canon,British Telecom and
musicians such as Ryan Teague and Jon Hopkins. In addition, an extensive
selection of archive images are available for licensing and printing and can
be viewed at Flickr.com

commercial web site http://www.lindengledhill.com/

Linden has a Ph.D in biochemistry from the University of Nottingham (UK) and
is the VP of Medicine and Process Delivery (MPD) at Glaxosmithkline based
in Philadelphia. MPD team members lead multidisciplinary projects teams for
the development of biopharmaceutical products for a wide variety of diseases
such as cancer, diabetes and rheumatoid arthritis.

Please see our Facebook page for further meeting details
https://www.facebook.com/PhiladelphiaSocietyforMicroscopy/




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From: microscopy.listserver-at-gmail.com
Date: Wednesday, April 6, 2016, 4:56 PM
Subject: [Microscopy] Lubricating instruments

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

On the same order:

Our Tecnai G20 has past the 10 years flag and I am wondering which moving piece we need to care of/service.
In the microscope itself there are only few moving pieces, some of which we almost never move (apertures) but an important one is the goniometer and it is moving all the time.
I still have no problem with it but I would like to prevent them instead of having to cure them later so I would like to know what is advised, what is your feedback with goniometers?
Do they need any service at all or are they good to go for the lifetime of the system?
Of less concern is the trackball to move the goniometer, but still I wonder if I need to clean any part inside before it stops working? It is a matter of fact that the air is filtered and very clean so the material does not take a lot of dust even after 10 years but still I would like to know.

Many thanks in advance for the answers and no thank you at all for the flow of automatic replies.

Regards
Stephane





--------------------------------------------
On Wed, 4/6/16, jkrupp-at-deltacollege.edu {jkrupp-at-deltacollege.edu} wrote:


Does anyone have advice about lubricating lab instruments?

We often have controls, gears, sliding blocks, shafts that
seem to need a little help. Nothing like a big overhaul or
major service, just a little tightness.

I know about the need for special lubricants in specific
places, but do you think it would be OK to try to loosen up
some things by applying some lubricant to specific places?

Have you found anything that seems to work well and is
compatible with scientific applications?

Thanks

Jon

Jonathan Krupp
ASB&T
San Joaquin Delta College
5151Pacific Ave.
Stockton, CA 95207
(209) 954-5284
jkrupp-at-deltacollege.edu



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From: microscopy.listserver-at-gmail.com
Date: April 6, 2016 at 11:59:58 AM CDT
Subject: [Microscopy] Hitachi S-415A schematics

Contents Retrieved from Microscopy Listserver Archives
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Hi,

I am starting the adventure of restoring an old and dilapidated
Hitachi S-415A SEM (for hobbyist use).

Amongst major work, E-T detector coatings have been practically
removed, power supply is damaged (someone apparently decided to use
an angle grinder on it) and lots of mechanical misc work all over.

Does anybody have a set of schematic diagrams for the S-415A
electronics that could send to me ?

Any help will be greatly appreciated!

--
Sebastian Diaz
Houston, TX
mobile: 832-470-0386

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From: microscopy.listserver-at-gmail.com
Date: April 6, 2016 at 11:31:55 AM CDT
Subject: [Microscopy] Re: Lubricating instruments

Contents Retrieved from Microscopy Listserver Archives
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Jon,

I've found a small amount of white lithium grease works for sliding
surfaces of focusing racks, moving blocks in microscope heads for
picking camera/eye, things like that. Even used a bit of Braycote (a
true high-vacuum grease) to stop the EDS on our STEM from screaming as
it inserted/retracted. A *tiny* bit - stuff is hideously expensive.

Phil

Does anyone have advice about lubricating lab instruments?

We often have controls, gears, sliding blocks, shafts that seem to need a little help. Nothing like a big overhaul or major service, just a little tightness.

I know about the need for special lubricants in specific places, but do you think it would be OK to try to loosen up some things by applying some lubricant to specific places?

Have you found anything that seems to work well and is compatible with scientific applications?

Thanks

Jon

Jonathan Krupp
ASB&T
San Joaquin Delta College
5151Pacific Ave.
Stockton, CA 95207
(209) 954-5284
jkrupp-at-deltacollege.edu

--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

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From: microscopy.listserver-at-gmail.com
Date: April 6, 2016 at 11:25:58 AM CDT
Subject: [Microscopy] Lubricating instruments

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Jon,
We used molybdenum disulfide paste for such jobs in our surface science lab.
No vapors and no spreading, like oils do. Also works for lubricating nuts
and bolts that might seize after equipment baking. Comes in little tubes.

Regards,
Larry Scipioni

-----Original Message-----
X-from: jkrupp-at-deltacollege.edu [mailto:jkrupp-at-deltacollege.edu]
Sent: Wednesday, April 06, 2016 12:08 PM
To: LES-at-ZSGENETICS.COM

Does anyone have advice about lubricating lab instruments?

We often have controls, gears, sliding blocks, shafts that seem to need a
little help. Nothing like a big overhaul or major service, just a little
tightness.

I know about the need for special lubricants in specific places, but do you
think it would be OK to try to loosen up some things by applying some
lubricant to specific places?

Have you found anything that seems to work well and is compatible with
scientific applications?

Thanks

Jon

Jonathan Krupp
ASB&T
San Joaquin Delta College
5151Pacific Ave.
Stockton, CA 95207
(209) 954-5284
jkrupp-at-deltacollege.edu



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From: microscopy.listserver-at-gmail.com
Date: April 6, 2016 at 10:47:50 AM CDT
Subject: [Microscopy] Lubricating instruments

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Does anyone have advice about lubricating lab instruments?

We often have controls, gears, sliding blocks, shafts that seem to need a little help. Nothing like a big overhaul or major service, just a little tightness.

I know about the need for special lubricants in specific places, but do you think it would be OK to try to loosen up some things by applying some lubricant to specific places?

Have you found anything that seems to work well and is compatible with scientific applications?

Thanks

Jon

Jonathan Krupp
ASB&T
San Joaquin Delta College
5151Pacific Ave.
Stockton, CA 95207
(209) 954-5284
jkrupp-at-deltacollege.edu



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From: microscopy.listserver-at-gmail.com
Date: April 6, 2016 at 8:18:39 AM CDT
Subject: [Microscopy] Transmission Electron Microscopy short course May 17-19, 2016

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Greetings,

Hooke College of Applied Sciences, located in Westmont, IL, is offering a Transmission Electron Microscopy short course May 17-19, 2016.

In addition to lectures, this course emphasizes hands-on training using state-of-the-art equipment.

For further TEM training details and registration information, please follow the link below:
https://www.mccrone.com/transmission-electron-microscopy-tem-course


Chris Gorman
P. 630.887.7100



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From: microscopy.listserver-at-gmail.com
Date: April 4, 2016 at 7:00:14 PM CDT
Subject: [Microscopy] viaWWW:Shadow casting using Denton Desk II sputter coater.

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Title-Subject: [Filtered] Shadow casting using Denton Desk II sputter coater.

Message: We have Denton Desk II Sputter coater. It is old and basic model for sputter coating to
prepare sample for SEM. I have one user, who is interested in study of extracellular matrix protein
by shadow casting TEM.
So, I wanted to know that what is the minimum thickness can be achieved with Denton Desk II sputter
coater using what parameters. Here, we can have control on current and sputtering time only and we
can get max vacuum of 100 mTorr.

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From: microscopy.listserver-at-gmail.com
Date: April 2, 2016 at 2:41:21 PM CDT
Subject: [Microscopy] RE: viaWWW:Fixing Insects for TEM

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Jorge,

Your problem is most likely one of 2 things:
1) The wing surface is setose or highly setose and trapping air or water, thus preventing the resin from penetrating the wing cuticle.
or
2) The lipids covering the external surface of the exoskeleton are also coating the wing surface and are not being removed during the alcohol dehydration steps.

For either case, I would first try adding a drop or two of non-ionic detergent (like Triton X-100 or Tween 80) to the fixative. This will act as a surfactant and help get the alcohol to the wing surface. It may also help solubilize the lipids coating the wing, helping with their removal.
This may take some experimentation - a "drop or two" may not be enough. If the wings sink in the fixative, that is usually a good indication of "enough". But, you may need to use a more non-polar (organic) solvent to remove any lipid coating. With luck, a longer chain alcohol like tert-butyl will work.

Phil

Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576


Email: blayjorge-at-gmail.com
Name: Jorge Santiago-Blay

Organization: NMNH

Title-Subject: [Filtered] Fixing nsects for TEM

Message: We are trying to fix insect wings for TEM. It appears that something in the exoskeleton is
making it harder wing to remain attached to the resin (of the block).

If you have any constructive suggestions, please send them to blayjorge-at-gmail.com

Gratefully,

Jorge


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From: microscopy.listserver-at-gmail.com
Date: Tuesday 7 June
Subject: [Microscopy] viaWWW:Workshop microscopy data analysis with Python & HyperSpy

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Email: a.helvoort-at-ntnu.no
Name: Ton van Helvoort

Organization: NTNU/TEM Gemini Centre

Title-Subject: [Filtered] Workshop microscopy data analysis with Python & HyperSpy

Message: The hands-on workshop, in connection with SCANDEM 2016, will address the basics of
multi-dimensional data analysis using HyperSpy (http://hyperspy.org/). Most examples will be taken
from the field of electron microscopy and will include EDX, EELS, image and diffraction analysis
using conventional and machine learning methods. The same methodology should be directly applicable
to other domains. No previous knowledge of the Python programming language is required.


Registration closes 1st May or when the places are filled. (Note: Early-bird registration and
abstract submission deadline for SCANDEM: 8 April)

HyperSpy is a Python library developed by active microscopists allowing basic and advanced data
analysis. The Python programming language is rapidly establishing itself as the lingua franca in
most areas of scientific computing, including microscopy.

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From: microscopy.listserver-at-gmail.com
Date: April 1, 2016 at 6:02:44 PM CDT
Subject: [Microscopy] viaWWW:Fixing Insects for TEM

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Email: blayjorge-at-gmail.com
Name: Jorge Santiago-Blay

Organization: NMNH

Title-Subject: [Filtered] Fixing nsects for TEM

Message: We are trying to fix insect wings for TEM. It appears that something in the exoskeleton is
making it harder wing to remain attached to the resin (of the block).

If you have any constructive suggestions, please send them to blayjorge-at-gmail.com

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Jorge

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From: microscopy.listserver-at-gmail.com
Date: April 1, 2016 at 2:50:20 PM CDT
Subject: [Microscopy] Re: Pricce of early EMs

Contents Retrieved from Microscopy Listserver Archives
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The ANL AAEM (VG HB603Z) the first 300 kV CFEG DSTEM/CTEM/AEM was
purchased in 1984, it's price tag was ~ $1.5M. The instrument
included: STEM, TEM (yes TEM in a VG!), SCEM, SEM, XEDS, EELS, AUGER,
SIMS, LEED, Prep Chamber, TF Evaporators, High Pressure Gas Reaction
Cell, CCD imaging/diffraction camera, Hot/Cold/RT/Be Dble tilt holders,
......

After 20+ years, the instrument's "last light" was on Nov 19, 2008 and
at decommissioning it was still operating at 300 kV. It's parts were
redistributed and still populate several research instruments (some of
which are not electron microscopes).

Nestor


--
===========================================

Dr. Nestor J. Zaluzec
Argonne National Laboratory
Electron Microscopy Center
Center for Nanoscale Materials
NanoScience and Technology Division
Bldg 212 / A-143
9700 S. Cass Ave
Argonne, Illinois 60439 USA

Tel: 530-NES-TORZ (530-637-8679) has Voice Mail
Lab: 630-252-7901


iChat: Zaluzec-at-AIM.com {mailto:Zaluzec-at-aim.com}
Skype: Zaluzec
Polycom: 146.139.72.119
TPM: http://tpm.amc.anl.gov

Email: Zaluzec-at-aaem.amc.anl.gov {mailto:Zaluzec-at-aaem.amc.anl.gov}

Senior Scientist - Argonne National Laboratory
Fellow of the Microscopy Society of America
Senior Fellow the Computational Institute - University of Chicago
E.P. Wigner Fellow - Oak Ridge National Laboratory
Past President Microscopy Society of America
Adjunct Professor of Physics - Northern Illinois University &
the University of Illinois at Chicago
Visiting Professor of Microscopy - Manchester University

===========================================
TPMLab: http://tpm.amc.anl.gov
MMSite: http://www.amc.anl.gov
===========================================

The box said ...
"This program requires Win 95/98/NT or better..."
So I bought a Mac !

===========================================

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--
===========================================

Dr. Nestor J. Zaluzec
Argonne National Laboratory
Electron Microscopy Center
Center for Nanoscale Materials
NanoScience and Technology Division
Bldg 212 / A-143
9700 S. Cass Ave
Argonne, Illinois 60439 USA

Tel: 530-NES-TORZ (530-637-8679) has Voice Mail
Lab: 630-252-7901


iChat: Zaluzec-at-AIM.com
Skype: Zaluzec
Polycom: 146.139.72.119
TPM: http://tpm.amc.anl.gov

Email: Zaluzec-at-aaem.amc.anl.gov

Senior Scientist - Argonne National Laboratory
Fellow of the Microscopy Society of America
Senior Fellow the Computational Institute - University of Chicago
E.P. Wigner Fellow - Oak Ridge National Laboratory
Past President Microscopy Society of America
Adjunct Professor of Physics - Northern Illinois University &
the University of Illinois at Chicago
Visiting Professor of Microscopy - Manchester University

===========================================
TPMLab: http://tpm.amc.anl.gov
MMSite: http://www.amc.anl.gov
===========================================

The box said ...
"This program requires Win 95/98/NT or better..."
So I bought a Mac !

===========================================

From: {John.Mardinly-at-asu.edu}

Yes, they were a lot cheaper when half the price of a microscope was not add-ons from Gatan. Of course, they did a lot less.

John Mardinly, Retired from ASU





On Mar 31, 2016, at 7:16 PM, bigelow-at-umich.edu wrote:




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In the early 1960s I purchased a JEOL JEM6A TEM that operated at 80 and
100 KV and was fully equipped (rotating-tilting stage, heating stage,
tensile deformation stage, and even a Cine camera) for $35K. It had a
guaranteed resolution of 20A, with a stated potential of reaching 10A
under ideal external conditions. This microscope served the teaching
and research needs of the graduate sstudents and faculty of our
deartment for thirty years. This was one of the first TEMS to compete
with the Siemans TEMs. Hitachi made a similar instrument that sold for a
similar price. At about that time RCA sold a Model EML TEM that
operated at 50KV, and was specifically designed for biological
applications, at a much lower price.

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From: microscopy.listserver-at-gmail.com
Date: April 1, 2016 at 2:12:08 PM CDT
Subject: Re: [Microscopy] Historical: Price of Microscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The ANL AAEM (VG HB603Z) the first 300 kV CFEG DSTEM/CTEM/AEM was
purchased in 1984, it's price tag was ~ $1.5M. The instrument
included: STEM, TEM (yes TEM in a VG!), SCEM, SEM, XEDS, EELS, AUGER,
SIMS, LEED, Prep Chamber, TF Evaporators, High Pressure Gas Reaction
Cell, CCD imaging/diffraction camera, Hot/Cold/RT/Be Dble tilt holders,
......

After 20+ years, the instrument's "last light" was on Nov 19, 2008 and
at decommissioning it was still operating at 300 kV. It's parts were
redistributed and still populate several research instruments (some of
which are not electron microscopes).

Nestor


--
===========================================

Dr. Nestor J. Zaluzec
Argonne National Laboratory
Electron Microscopy Center
Center for Nanoscale Materials
NanoScience and Technology Division
Bldg 212 / A-143
9700 S. Cass Ave
Argonne, Illinois 60439 USA

Tel: 530-NES-TORZ (530-637-8679) has Voice Mail
Lab: 630-252-7901


iChat: Zaluzec-at-AIM.com
Skype: Zaluzec
Polycom: 146.139.72.119
TPM: http://tpm.amc.anl.gov

Email: Zaluzec-at-aaem.amc.anl.gov

Senior Scientist - Argonne National Laboratory
Fellow of the Microscopy Society of America
Senior Fellow the Computational Institute - University of Chicago
E.P. Wigner Fellow - Oak Ridge National Laboratory
Past President Microscopy Society of America
Adjunct Professor of Physics - Northern Illinois University &
the University of Illinois at Chicago
Visiting Professor of Microscopy - Manchester University

===========================================
TPMLab: http://tpm.amc.anl.gov
MMSite: http://www.amc.anl.gov
===========================================

The box said ...
"This program requires Win 95/98/NT or better..."
So I bought a Mac !

===========================================

==============================Original Headers==============================
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14, 48 -- To: microscopy-at-microscopy.com
14, 48 -- From: "Nestor J. Zaluzec - ANL" {zaluzec-at-aaem.amc.anl.gov}
14, 48 -- Subject: Historical: Price of Microscopes
14, 48 -- Message-ID: {56FEAF8B.1000305-at-aaem.amc.anl.gov}
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==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: April 1, 2016 at 1:19:45 PM CDT
Subject: Re: [Microscopy] Historical: Price of Microscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The ANL AAEM (VG HB603Z) the first 300 kV CFEG DSTEM/CTEM/AEM was
purchased in 1984, it's price tag was ~ $1.5M. The instrument
included: STEM, TEM (yes TEM in a VG!), SCEM, SEM, XEDS, EELS, AUGER,
SIMS, LEED, Prep Chamber, TF Evaporators, High Pressure Gas Reaction
Cell, CCD imaging/diffraction camera, Hot/Cold/RT/Be Dble tilt holders,
......

After 20+ years, the instrument's "last light" was on Nov 19, 2008 and
at decommissioning it was still operating at 300 kV. It's parts were
redistributed and still populate several research instruments (some of
which are not electron microscopes).

Nestor


--
===========================================

Dr. Nestor J. Zaluzec
Argonne National Laboratory
Electron Microscopy Center
Center for Nanoscale Materials
NanoScience and Technology Division
Bldg 212 / A-143
9700 S. Cass Ave
Argonne, Illinois 60439 USA

Tel: 530-NES-TORZ (530-637-8679) has Voice Mail
Lab: 630-252-7901


iChat: Zaluzec-at-AIM.com
Skype: Zaluzec
Polycom: 146.139.72.119
TPM: http://tpm.amc.anl.gov

Email: Zaluzec-at-aaem.amc.anl.gov

Senior Scientist - Argonne National Laboratory
Fellow of the Microscopy Society of America
Senior Fellow the Computational Institute - University of Chicago
E.P. Wigner Fellow - Oak Ridge National Laboratory
Past President Microscopy Society of America
Adjunct Professor of Physics - Northern Illinois University &
the University of Illinois at Chicago
Visiting Professor of Microscopy - Manchester University

===========================================
TPMLab: http://tpm.amc.anl.gov
MMSite: http://www.amc.anl.gov
===========================================

The box said ...
"This program requires Win 95/98/NT or better..."
So I bought a Mac !

===========================================

==============================Original Headers==============================
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14, 48 -- From: "Nestor J. Zaluzec - ANL" {zaluzec-at-aaem.amc.anl.gov}
14, 48 -- Subject: Historical: Price of Microscopes
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==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: April 1, 2016 at 12:27:02 PM CDT
Subject: [Microscopy] Historical: Price of Microscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The ANL AAEM (VG HB603Z) the first 300 kV CFEG DSTEM/CTEM/AEM was
purchased in 1984, it's price tag was ~ $1.5M. The instrument
included: STEM, TEM (yes TEM in a VG!), SCEM, SEM, XEDS, EELS, AUGER,
SIMS, LEED, Prep Chamber, TF Evaporators, High Pressure Gas Reaction
Cell, CCD imaging/diffraction camera, Hot/Cold/RT/Be Dble tilt holders,
......

After 20+ years, the instrument's "last light" was on Nov 19, 2008 and
at decommissioning it was still operating at 300 kV. It's parts were
redistributed and still populate several research instruments (some of
which are not electron microscopes).

Nestor


--
===========================================

Dr. Nestor J. Zaluzec
Argonne National Laboratory
Electron Microscopy Center
Center for Nanoscale Materials
NanoScience and Technology Division
Bldg 212 / A-143
9700 S. Cass Ave
Argonne, Illinois 60439 USA

Tel: 530-NES-TORZ (530-637-8679) has Voice Mail
Lab: 630-252-7901


iChat: Zaluzec-at-AIM.com
Skype: Zaluzec
Polycom: 146.139.72.119
TPM: http://tpm.amc.anl.gov

Email: Zaluzec-at-aaem.amc.anl.gov

Senior Scientist - Argonne National Laboratory
Fellow of the Microscopy Society of America
Senior Fellow the Computational Institute - University of Chicago
E.P. Wigner Fellow - Oak Ridge National Laboratory
Past President Microscopy Society of America
Adjunct Professor of Physics - Northern Illinois University &
the University of Illinois at Chicago
Visiting Professor of Microscopy - Manchester University

===========================================
TPMLab: http://tpm.amc.anl.gov
MMSite: http://www.amc.anl.gov
===========================================

The box said ...
"This program requires Win 95/98/NT or better..."
So I bought a Mac !

===========================================

==============================Original Headers==============================
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14, 48 -- From: "Nestor J. Zaluzec - ANL" {zaluzec-at-aaem.amc.anl.gov}
14, 48 -- Subject: Historical: Price of Microscopes
14, 48 -- Message-ID: {56FEAF8B.1000305-at-aaem.amc.anl.gov}
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From: microscopy.listserver-at-gmail.com
Date: April 1, 2016 at 10:13:37 AM CDT
Subject: [Microscopy] RE: Pricce of early EMs

Contents Retrieved from Microscopy Listserver Archives
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The JEM-6A was pretty old TEM. I used JEM-6C until 1985 for observation on biology samples. It used many vacuum tubes and big resistors and we have to put those things in separated room. On 1986, we bought JEM-1200EX with ASID10 (SEM/STEM attachment). I paid $450K.

Vincent Wang
Schafer Vallecitos Laboratory

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In the early 1960s I purchased a JEOL JEM6A TEM that operated at 80 and
100 KV and was fully equipped (rotating-tilting stage, heating stage, tensile deformation stage, and even a Cine camera) for $35K. It had a guaranteed resolution of 20A, with a stated potential of reaching 10A under ideal external conditions. This microscope served the teaching and research needs of the graduate sstudents and faculty of our deartment for thirty years. This was one of the first TEMS to compete with the Siemans TEMs. Hitachi made a similar instrument that sold for a similar price. At about that time RCA sold a Model EML TEM that operated at 50KV, and was specifically designed for biological applications, at a much lower price.

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From: microscopy.listserver-at-gmail.com
Date: April 1, 2016 at 8:06:49 AM CDT
Subject: [Microscopy] viaWWW:Application Engineer / Research Scientist Position in Optical

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Email: prevedel-at-embl.de
Name: Robert Prevedel

Organization: EMBL Heidelberg

Title-Subject: [Filtered] Application Engineer / Research Scientist Position in Optical Microscopy

Message: Job Description

The European Molecular Biology Laboratory (EMBL) is one of the highest ranked scientific research
organisations in the world. The Headquarters Laboratory is located in Heidelberg (Germany), with
additional sites in Grenoble (France), Hamburg (Germany), Hinxton (UK) and Monterotondo (Italy).

The Application Engineer / Research Scientist will join the Prevedel group. The goal of the lab is
to develop next generation, innovative optical imaging techniques to push the frontier of deep
tissue microscopy. The research is geared towards direct applications in biology and neuroscience.
The major task of the Application Engineer / Research Scientist will be to support the group by
contributing to its research projects and laboratory management. This involves:
Design of mechanical, optical as well as electronic components of cutting-edge, novel light
microscopes to collect three-dimensional datasets inside scattering tissue.

Construction and adaptation of custom microscopy setups for specific life science applications and
imaging conditions.
Programming and customizing computational data analysis packages including three-dimensional
visualization of imaging data and analysis methods.

Improving image acquisition workflows (e.g. hardware automation, image post-processing and analysis)
and ensuring a technology watch to keep the microscopes at a cutting edge level (at the hardware and
software levels).
Working in close collaboration with biologists and neuroscientists to ensure practicality of the
microscopes (e.g. custom sample mounts, sample environment control)

The Application Engineer / Research Scientist will directly participate in the groups research
projects and will be responsible for training and providing technical assistance for students and
postdoctoral fellows in the group. In addition, the Application Engineer / Research Scientist will
be in charge of the general lab organization that includes ordering of supplies and equipment as
well as the maintenance of databases.

Qualifications and Experience

The ideal candidate should be an optical engineer or physicist (master degree or equivalent, a PhD
would be an advantage) possessing advanced computer skills. The candidate should ideally have an
expertise in advanced optics, imaging/spectroscopy or light microscopy, must be comfortable with
complex technical equipment (including pulsed lasers) and preferably possess programming (Matlab,
Python, LabView, or similar) and/or simulation skills (CAD, ZEMAX, or similar). A keen interest in
biological applications of new optical technologies as well as experience in supervising students
and laboratory organization would be an advantage.

The successful candidate should be able to work independently, be very well organized, reliable and
enjoy working in an international team within a highly collaborative atmosphere. Excellent
communication and presentation skills and fluency in English are required.

Application Instructions

Please apply online through www.embl.org/jobs

Additional Information

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appropriate to an international research organisation.

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From: microscopy.listserver-at-gmail.com
Date: April 1, 2016 at 7:36:07 AM CDT
Subject: [Microscopy] Re:Detecting limit by EDS elemental analysis.

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Hi guys!

Nice answers... but nobody asked Ravi what is precisely is sample and
this is an essential information for a sound answer!

First the optimum X-Ray line and beam energy will not be the same if the
sample is
- a bulk homogeneous material or
- a thin (how thin) film on a bulk substrate or (micro/nano-) particles
on a bulk substrate or even -
- a thin foil like for TEM
To be sure that you analyze the volume containing the elements you are
interested in and only that volume you should run a Monte-Carlo simulation.

Second: The nature of the matrix may also influence the choice for X-ray
line once you take the absorption consideration and possible overlap
with adjacent lines from other elements.

Kind regards

Philippe Buffat

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From: microscopy.listserver-at-gmail.com
Date: March 31, 2016 at 9:57:45 PM CDT
Subject: [Microscopy] RE: Pricce of early EMs

Contents Retrieved from Microscopy Listserver Archives
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When I started at Wool Research Organisation New Zealand in 2003 (now AgResearch), we had a gorgeous Philips EM300 which had been bought new in 1968. It kept working (with help in the form of some organ transplants from friends at The University of Otago) until 2006 when we decommissioned.

I have the brochure replete with photographs of an attractive young (female) scientist peering devotedly into the instrument and changing the wehnalt assembly etc. It was a top range 80-100 kV TEM of its day. The handwritten notes on the brochure margins indicate $41,000, presumably New Zealand Dollars (newly introduced the year before in a change from Pounds). When introduced a NZ$ was apparently valued at an NZ pound (which had been pegged to the British pound). Educated guess is therefore about GPB20,000 for the instrument back in the day.

Hopefully that stacks up.

Kind regards
Duane

AgResearch Limited | Lincoln Research Centre | New Zealand
Dr Duane P Harland, Senior Scientist
T +64 3 321 8710 E duane.harland-at-agresearch.co.nz




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From: microscopy.listserver-at-gmail.com
Date: March 31, 2016 at 8:59:39 PM CDT
Subject: [Microscopy] Pricce of early EMs

Contents Retrieved from Microscopy Listserver Archives
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In the early 1960s I purchased a JEOL JEM6A TEM that operated at 80 and
100 KV and was fully equipped (rotating-tilting stage, heating stage,
tensile deformation stage, and even a Cine camera) for $35K. It had a
guaranteed resolution of 20A, with a stated potential of reaching 10A
under ideal external conditions. This microscope served the teaching
and research needs of the graduate sstudents and faculty of our
deartment for thirty years. This was one of the first TEMS to compete
with the Siemans TEMs. Hitachi made a similar instrument that sold for a
similar price. At about that time RCA sold a Model EML TEM that
operated at 50KV, and was specifically designed for biological
applications, at a much lower price.

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From: microscopy.listserver-at-gmail.com
Date: March 31, 2016 at 8:34:42 PM CDT
Subject: [Microscopy] viaWWW:Looking to purchase a JEOL 2010F gun/tank/microscope

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Email: nigel.browning-at-pnnl.gov
Name: Nigel Browning

Organization: Pacific Northwest National Laboratory

Title-Subject: [Filtered] Looking to purchase a JEOL 2010F gun/tank/microscope

Message: I am looking to find spare parts for a JEOL 200kV field emission TEM. In particular I am
interested in the gun and the tank but I can also purchase the whole system if someone is interested
in selling it. I would be willing to pay for all stages of the dismantling and shipping of the
instrument to PNNL as well as a purchase price for the microscope. Any current condition for the
microscope will be considered as we will be looking to rip it apart anyway.

Nigel

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From: microscopy.listserver-at-gmail.com
Date: March 31, 2016 at 8:33:30 PM CDT
Subject: [Microscopy] viaWWW:UMB Current Electron Microscopy Techniques Workshop May 24 -

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Email: rhsia-at-umaryland.edu
Name: Ru-ching Hsia

Organization: University of Maryland, Baltimore

Title-Subject: [Filtered] UMB Current Electron Microscopy Techniques Workshop May 24 - 25, 2016

Message: Dear Colleagues,

We are pleased to announce that the University of Maryland Baltimore will be hosting the Third
Annual Current EM Techniques Workshop on May 24 and 25, 2016. The purpose of this annual workshop is
to introduce participants to new electron microscopy techniques and instruments via live
demonstration and open discussion. The focus of this years workshop is Correlative LM & EM. The
workshop will include oral presentations and Tips-and-Tricks Technology forums on the first day and
live instrument demonstration on the second day. Dr. Lucy Collison of Francis Crick Institute and
Richard Schalek of Harvard University will be the keynote speakers. Demo instruments will feature
ATUMtome of RMC-Boeckeler, ASP-1000-IGL of Microscopy Innovations, Pelco Biowave Pro Microwave
System of TedPella, CryoEM sample preparation of Leica Microsystems, Polyo III, Lynx Tissue
Processor of EMS, cryo-TEM, cryo-SEM. Live Serial Block Face sectioning will be viewed via weblink.

A dinner event will be co-sponsored by the Chesapeake Microscopy and Microanalysis society (CMMS) on
May 24th providing opportunities for social and scientific exchange among workshop participants and
CMMS members.

More information: http://www.dental.umaryland.edu/2016currentemtechniquesworkshop/

Registrations: https://umbcurrentemtechniqueworkshop.eventbrite.com

Inquiry: coreimaging-at-umaryland.edu

We look forward to seeing you in May.


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From: microscopy.listserver-at-gmail.com
Date: March 31, 2016 at 12:42:49 PM CDT
Subject: [Microscopy] Detecting limit by EDS elemental analysis.

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I partially agree with Nicolas.

XRF will be easier for detection since it won't have the same prominent background (bremstrallung) that electron beam excitation has. Therefore, peaks will be more detectable.

You should be using a lower voltage. The factor of 2.5 is a good rule of thumb. You should be able to see the Zn L line well enough with a thin window detector. I can hardly envision using a Be window detector anymore. I really like seeing the elements down to Be. I routinely depend on seeing the C and O lines. I spend most of my time at 10 kV anymore and sometimes drop it further.

However, I think you should be able to see peaks corresponding to a tenth percent or so by EDS. The limit will be how well the background is defined. I routinely collect at 15-20 kcps for a minute. I will count longer in critical situations where I need to improve the detection limit.

Warren
----------------------------------------------------------------------------

Hi Ravi,
EDS is not the best to detect element in trace (if there is less than 1%). It's easier by fluorescence when the SEM has an Xray source or by WDS.
Anyway I think you can do better to optimize the conditions because 30KV is not suitable (except to see the L alpha of Pb).
An empiric rule says that the best accelerating voltage is 2.5 times the energy of the peak you expect to analyse. This is because the ionization efficiency of an atom by an electron is nearly maximum when the energy of the electron is between 2 or 3 times the energy of ionization.
Applying this rule you may get more photons on :
Pb M alpha near 5KV
Pb L alpha near 30KV
Cd L alpha near 9KV
Zn K alpha near 24KV
Zn L alpha near 3KV (but probably to low voltage to get a good count rate. So you can try 5KV) Once the accelerating voltage adjusted, increase the beam current to increase the count rate and acquire the spectrum during long time (may be 300 secondes).

Nicolas STEPHANT

Universite de Nantes
Institut Jean Rouxel
Service de microscopie electronique a balayage et microanalyse
2 rue de la Houssiniere
BP 92208
44322 Nantes cedex 3

Tel : 02 40 37 64 26
Mel : nicolas.stephant-at-univ-nantes.fr
Web : http://cnrs-imn.fr

"Le monde n'existe que pour autant que nous sommes capables d'en produire une image"
C.G Jung

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Email: ravi.thakkar369-at-gmail.com
Name: Ravi

Title-Subject: [Filtered] Detecting limit by EDS elemental analysis.

Message: I did EDS analysis by SEM and I was interested to find Pb,Cd
and Zn elements but unfortunately my sample did not show peaks of any
of these element. I used optimum parameters like
30 KV acceleration voltage 10 mm working distance on S3500N Hitachi
SEM Oxford EDS detector 10 mm2 window.

What is the detecting limit of SEM-EDS for Pb,CD and Zn. (in
concentration)? May I have a chart or any reference document where I
can study the general detecting limit of EDS by TEM and SEM for all the elements.

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From: microscopy.listserver-at-gmail.com
Date: March 31, 2016 at 12:09:37 PM CDT
Subject: [Microscopy] Re: viaWWW: Historical Cost of electron microscopes

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According to my old advisor, Will Bigelow, the JEOL 100CX TEM/STEM that I used for my thesis cost $300K back in 1977. Seemed like a lot of money back then.

John Mardinly, Retired from ASU




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Email: paulwebsterphd-at-gmail.com
Name: Paul Webster

Organization: Oak Crest

Title-Subject: [Filtered] Cost of electron microscopes

Message: Hello listers,

I have a question posed by a colleague, which I think is better answered by the experts who read
this. Here is the question:

"What do you know about the pricing of electron microscopes in the early days? NMR started to get
really expensive in the late 1970's with the development of bigger and bigger superconducting
magnets. I am guessing that EM reached the same price points earlier. Could you venture a guess
about when it was that a commercial EM was first on the market for more than a million dollars?"

As a bonus question (from me), does anyone know when the first high-voltage microscopes came on to
the market, and what their cost was?

I'm writing from the MSA submission form because I am unable to get through using the regular
connection - I am subscribed so will see any messages posted to the list.

Thank you,

Paul Webster
Oak Crest Institute of Science
California



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From: microscopy.listserver-at-gmail.com
Date: March 31, 2016 at 12:05:34 PM CDT
Subject: [Microscopy] Re: viaWWW:Thermal evaporation with Emitech Carbon

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Tommi;
You can make a basket for metal by wrapping sone 600-700 micron tungsten wire around the end of a sheet metal screw, and just place the metal you want to evaporate in the basket. Use the same power supply used for carbon evaporation to heat the basket.

John Mardinly, Retired from ASU




On Mar 30, 2016, at 4:33 PM, microscopy.listserver-at-gmail.com wrote:




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Email: whiteto-at-missouri.edu
Name: Tommi A. White

Organization: University of Missouri Electron Microscopy Core

Title-Subject: [Filtered] Thermal evaporation with Emitech Carbon Evaporator

Message: Dear Microscopy List-serve,

In the absence of funding, we would like to try to modify our turbo pumped Emitech equipment (from
1999) into a thermal evaporator allowing better adherence of metal coating for our electron beam
lithography patterns.

We have both the sputtering system (K575X) and carbon evaporator (K950). In the carbon evaporator
manual, it says we can use for thermal evaporation but there are little instructions on how to
perform this conversion.

Does anyone have experience with converting this system for metal evaporation? Maybe a detailed
description of how this conversion could take place? Would anyone be willing to guide us on what we
need to do? We have an instrument repair shop and a machine shop on campus, so we can utilize their
expertise if we know what we need.

Thanks in advance for any input.
Tommi


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8, 43 -- Subject: Re: [Microscopy] viaWWW:Thermal evaporation with Emitech Carbon
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From: microscopy.listserver-at-gmail.com
Date: March 31, 2016 at 10:33:42 AM CDT
Subject: [Microscopy] Re:Detecting limit by EDS elemental analysis.

Contents Retrieved from Microscopy Listserver Archives
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Hi Ravi,
EDS is not the best to detect element in trace (if there is less than
1%). It's easier by fluorescence when the SEM has an Xray source or by WDS.
Anyway I think you can do better to optimize the conditions because 30KV
is not suitable (except to see the L alpha of Pb).
An empiric rule says that the best accelerating voltage is 2.5 times the
energy of the peak you expect to analyse. This is because the
ionization efficiency of an atom by an electron is nearly maximum when
the energy of the electron is between 2 or 3 times the energy of
ionization.
Applying this rule you may get more photons on :
Pb M alpha near 5KV
Pb L alpha near 30KV
Cd L alpha near 9KV
Zn K alpha near 24KV
Zn L alpha near 3KV (but probably to low voltage to get a good count
rate. So you can try 5KV)
Once the accelerating voltage adjusted, increase the beam current to
increase the count rate and acquire the spectrum during long time (may
be 300 secondes).

Nicolas STEPHANT

Universite de Nantes
Institut Jean Rouxel
Service de microscopie electronique a balayage et microanalyse
2 rue de la Houssiniere
BP 92208
44322 Nantes cedex 3

Tel : 02 40 37 64 26
Mel : nicolas.stephant-at-univ-nantes.fr
Web : http://cnrs-imn.fr

"Le monde n'existe que pour autant que nous sommes capables d'en produire une image"
C.G Jung

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Email: ravi.thakkar369-at-gmail.com
Name: Ravi

Title-Subject: [Filtered] Detecting limit by EDS elemental analysis.

Message: I did EDS analysis by SEM and I was interested to find Pb,Cd and Zn elements but
unfortunately my sample did not show peaks of any of these element. I used optimum parameters like
30 KV acceleration voltage 10 mm working distance on S3500N Hitachi SEM Oxford EDS detector 10 mm2
window.

What is the detecting limit of SEM-EDS for Pb,CD and Zn. (in concentration)? May I have a chart or
any reference document where I can study the general detecting limit of EDS by TEM and SEM for all
the elements.

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7, 26 -- Subject: Re:Detecting limit by EDS elemental analysis.
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From: microscopy.listserver-at-gmail.com
Date: March 31, 2016 at 9:52:48 AM CDT
Subject: [Microscopy] Re: Historical prices of EMs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Besides the hardware upgrades over the decades, let's remember the software to run it all. Software is a big part of the package--who does not expect a good UI?

My 2 cents.

Roseann

Roseann Csencsits
Group Leader-Microbeam Analyses
Schafer Vallecitos Laboratories, Inc.
6705 Vallecitos Rd.
Sunol, CA 94586
925-862-4345
roseann.csencsits-at-schafercorp.com

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EDS systems seem to me to be going up as well. I won't name companies, but I paid just over $65k in 2011 for the EDS system on my SEM, but was quoted $93k (after discount) for the EDS on my new S/TEM, and was recently quoted $147k+ for an EDS system on a FE-SEM.
Some of this increase must be due to the change to SDD detectors from
Si(Li) detectors, but not all of it.

Phil

On 03/30/2016 23:25 , joetherob-at-gmail.com wrote:



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Paul,

I have been in the commercial end of EM since the late 70's. I have worked for 4 different column companies and three EDS companies.

The price of a scanning electron microscope in the late seventies ranged from $15k for an ISI Mini SEM to $ 125,000 for a full featured SEM. TEMs were about $75,000 for a biological unit and $175,000 for a Philips 420 type System.

In the 80's the entry level SEMs were about $35 to 50,000. High end SEMs were still about $125,000. The onset of Technology changes (Field Emission SEMs and intermediate voltage TEMs) in about 1985 correlated migration of semiconductor companies to routine EM use. This saw the price of FE SEMs and IM TEMs to rise steeply. I was in sales for Philips in 1989 and a CM20 was about $400K while a CM30 was $500K. With the addition of STEM, EDS, and EELS, they could approach $900,000. On the SEM front, Hitachi took advantage of their early lead in field emission SEM by offering them at about $250,000.

Prices rose slowly through the 90s. I believe the first $ 1 million EMs were full 200 mm wafer inspection and CD SEMs. TEMs broke the barrier in the mid nineties with the introduction of Field Emission TEMs.

By the end of the nineties dualbeam systems were over $1,000,000. FE SEMs were up to $400K and TEMs started to hit the $1.5 million mark with the introduction of the Tecnai F30 and JEOL 2010F.

Now we see prices jump again in the early 2000s with the introduction of aberration corrected TEMs at 2 to $ 4 million. FE SEMs were up to $450K and a new generation of mini SEMs took hold at about $50,000. Prices have risen for newer technologies in the 2100s, while the cost of a full featured floor standing SEM has remained at about& 150,000.

Interestingly, the price of EDS has stayed at about the same price for 30 years: $45K.

Regards,
Joe Robinson

--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576




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From: microscopy.listserver-at-gmail.com
Date: March 31, 2016 at 7:31:32 AM CDT
Subject: [Microscopy] Re: Historical prices of EMs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

EDS systems seem to me to be going up as well. I won't name companies,
but I paid just over $65k in 2011 for the EDS system on my SEM, but was
quoted $93k (after discount) for the EDS on my new S/TEM, and was
recently quoted $147k+ for an EDS system on a FE-SEM.
Some of this increase must be due to the change to SDD detectors from
Si(Li) detectors, but not all of it.

Phil

On 03/30/2016 23:25 , joetherob-at-gmail.com wrote:



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Paul,

I have been in the commercial end of EM since the late 70's. I have worked for 4 different column companies and three EDS companies.

The price of a scanning electron microscope in the late seventies ranged from $15k for an ISI Mini SEM to $ 125,000 for a full featured SEM. TEMs were about $75,000 for a biological unit and $175,000 for a Philips 420 type System.

In the 80's the entry level SEMs were about $35 to 50,000. High end SEMs were still about $125,000. The onset of Technology changes (Field Emission SEMs and intermediate voltage TEMs) in about 1985 correlated migration of semiconductor companies to routine EM use. This saw the price of FE SEMs and IM TEMs to rise steeply. I was in sales for Philips in 1989 and a CM20 was about $400K while a CM30 was $500K. With the addition of STEM, EDS, and EELS, they could approach $900,000. On the SEM front, Hitachi took advantage of their early lead in field emission SEM by offering them at about $250,000.

Prices rose slowly through the 90s. I believe the first $ 1 million EMs were full 200 mm wafer inspection and CD SEMs. TEMs broke the barrier in the mid nineties with the introduction of Field Emission TEMs.

By the end of the nineties dualbeam systems were over $1,000,000. FE SEMs were up to $400K and TEMs started to hit the $1.5 million mark with the introduction of the Tecnai F30 and JEOL 2010F.

Now we see prices jump again in the early 2000s with the introduction of aberration corrected TEMs at 2 to $ 4 million. FE SEMs were up to $450K and a new generation of mini SEMs took hold at about $50,000. Prices have risen for newer technologies in the 2100s, while the cost of a full featured floor standing SEM has remained at about& 150,000.

Interestingly, the price of EDS has stayed at about the same price for 30 years: $45K.

Regards,
Joe Robinson

--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

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From: microscopy.listserver-at-gmail.com
Date: March 30, 2016 at 10:03:35 PM CDT
Subject: [Microscopy] Historical prices of EMs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Paul,

I have been in the commercial end of EM since the late 70's. I have worked for 4 different column companies and three EDS companies.

The price of a scanning electron microscope in the late seventies ranged from $15k for an ISI Mini SEM to $ 125,000 for a full featured SEM. TEMs were about $75,000 for a biological unit and $175,000 for a Philips 420 type System.

In the 80's the entry level SEMs were about $35 to 50,000. High end SEMs were still about $125,000. The onset of Technology changes (Field Emission SEMs and intermediate voltage TEMs) in about 1985 correlated migration of semiconductor companies to routine EM use. This saw the price of FE SEMs and IM TEMs to rise steeply. I was in sales for Philips in 1989 and a CM20 was about $400K while a CM30 was $500K. With the addition of STEM, EDS, and EELS, they could approach $900,000. On the SEM front, Hitachi took advantage of their early lead in field emission SEM by offering them at about $250,000.

Prices rose slowly through the 90s. I believe the first $ 1 million EMs were full 200 mm wafer inspection and CD SEMs. TEMs broke the barrier in the mid nineties with the introduction of Field Emission TEMs.

By the end of the nineties dualbeam systems were over $1,000,000. FE SEMs were up to $400K and TEMs started to hit the $1.5 million mark with the introduction of the Tecnai F30 and JEOL 2010F.

Now we see prices jump again in the early 2000s with the introduction of aberration corrected TEMs at 2 to $ 4 million. FE SEMs were up to $450K and a new generation of mini SEMs took hold at about $50,000. Prices have risen for newer technologies in the 2100s, while the cost of a full featured floor standing SEM has remained at about & 150,000.

Interestingly, the price of EDS has stayed at about the same price for 30 years: $45K.

Regards,
Joe Robinson




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From: microscopy.listserver-at-gmail.com
Date: March 30, 2016 at 8:08:56 PM CDT
Subject: [Microscopy] viaWWW:Detecting limit by EDS elemental analysis.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Ravi,

Perhaps the easiest way to look at detection limits is to use one of the
Monte Carlo spectrum simulation programs. There are a number of
programs available but you might want to try...

Nicholas Ritchie (NIST)
DTSA-II - http://www.cstl.nist.gov/div837/837.02/epq/dtsa2/

Raynaud Gauvin, Hendrix Demers - (McGill Univ.)
Win X-ray, MC X-ray - http://montecarlomodeling.mcgill.ca/

One caution about this software. They use the same models and databases
as the standardless quantification routines, so the same uncertainties
apply to the simulation as to standardless quant. That said, they can
be useful to get an idea of detection limits and experimental
feasibility.

Cheers,
Henk


--------------------

Hendrik O. Colijn
Center for Electron Microscopy and AnalysiS
The Ohio State University
1305 Kinnear Rd, Suite 100, Columbus, OH 43212

colijn.1-at-osu.edu 614/643-3458
cemas.osu.edu {about:cemas.osu.edu}

"Time is that quality of nature which keeps things from happening all at
once." (Ray Cummings - 1922)
Lately it doesn't seem to be working.



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Email: ravi.thakkar369-at-gmail.com
Name: Ravi

Title-Subject: [Filtered] Detecting limit by EDS elemental analysis.

Message: I did EDS analysis by SEM and I was interested to find Pb,Cd
and Zn elements but
unfortunately my sample did not show peaks of any of these element. I
used optimum parameters like
30 KV acceleration voltage 10 mm working distance on S3500N Hitachi SEM
Oxford EDS detector 10 mm2
window.

What is the detecting limit of SEM-EDS for Pb,CD and Zn. (in
concentration)? May I have a chart or
any reference document where I can study the general detecting limit of
EDS by TEM and SEM for all
the elements.

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17, 112 -- From: Henk Colijn {colijn.1-at-osu.edu}
17, 112 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} ,
17, 112 -- Ravi Thakkar
17, 112 -- {ravi.thakkar369-at-gmail.com}
17, 112 -- Subject: Re: [Microscopy] viaWWW:Detecting limit by EDS elemental analysis.
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From: microscopy.listserver-at-gmail.com
Date: March 30, 2016 at 6:11:56 PM CDT
Subject: [Microscopy] viaWWW:Detecting limit by EDS elemental analysis.

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Email: ravi.thakkar369-at-gmail.com
Name: Ravi

Title-Subject: [Filtered] Detecting limit by EDS elemental analysis.

Message: I did EDS analysis by SEM and I was interested to find Pb,Cd and Zn elements but
unfortunately my sample did not show peaks of any of these element. I used optimum parameters like
30 KV acceleration voltage 10 mm working distance on S3500N Hitachi SEM Oxford EDS detector 10 mm2
window.

What is the detecting limit of SEM-EDS for Pb,CD and Zn. (in concentration)? May I have a chart or
any reference document where I can study the general detecting limit of EDS by TEM and SEM for all
the elements.

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From: microscopy.listserver-at-gmail.com
Date: March 30, 2016 at 6:10:38 PM CDT
Subject: [Microscopy] viaWWW: Historical Cost of electron microscopes

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Email: paulwebsterphd-at-gmail.com
Name: Paul Webster

Organization: Oak Crest

Title-Subject: [Filtered] Cost of electron microscopes

Message: Hello listers,

I have a question posed by a colleague, which I think is better answered by the experts who read
this. Here is the question:

"What do you know about the pricing of electron microscopes in the early days? NMR started to get
really expensive in the late 1970's with the development of bigger and bigger superconducting
magnets. I am guessing that EM reached the same price points earlier. Could you venture a guess
about when it was that a commercial EM was first on the market for more than a million dollars?"

As a bonus question (from me), does anyone know when the first high-voltage microscopes came on to
the market, and what their cost was?

I'm writing from the MSA submission form because I am unable to get through using the regular
connection - I am subscribed so will see any messages posted to the list.

Thank you,

Paul Webster
Oak Crest Institute of Science
California



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From: microscopy.listserver-at-gmail.com
Date: March 30, 2016 at 6:09:27 PM CDT
Subject: [Microscopy] viaWWW:Thermal evaporation with Emitech Carbon Evaporator

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Email: whiteto-at-missouri.edu
Name: Tommi A. White

Organization: University of Missouri Electron Microscopy Core

Title-Subject: [Filtered] Thermal evaporation with Emitech Carbon Evaporator

Message: Dear Microscopy List-serve,

In the absence of funding, we would like to try to modify our turbo pumped Emitech equipment (from
1999) into a thermal evaporator allowing better adherence of metal coating for our electron beam
lithography patterns.

We have both the sputtering system (K575X) and carbon evaporator (K950). In the carbon evaporator
manual, it says we can use for thermal evaporation but there are little instructions on how to
perform this conversion.

Does anyone have experience with converting this system for metal evaporation? Maybe a detailed
description of how this conversion could take place? Would anyone be willing to guide us on what we
need to do? We have an instrument repair shop and a machine shop on campus, so we can utilize their
expertise if we know what we need.

Thanks in advance for any input.
Tommi


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From: microscopy.listserver-at-gmail.com
Date: March 28, 2016 at 9:59:14 PM CDT
Subject: [Microscopy] viaWWW:Postdoctoral Position in Electron Microscopy

Contents Retrieved from Microscopy Listserver Archives
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Dear Early Career Scholars

The EPMA 2016 Topical Conference held at the University of Wisconsin,
Madison May 16 - 19 can provide up to $750 in reimbursement for
travel, lodging, and registration through a NSF grant. The NSF funds
available for ECS financial support can only be used for awardees in
the USA. ECS includes undergraduates, graduates, post docs, and
professionals within 2 years of their terminal degree. No abstract
is required to receive the funds. However, a letter of support from an
advisor/employer/supervisor is needed and an example is provided
below. Awards will be granted on a weekly basis until the funds have
been fully committed.

http://www.microanalysissociety.org/topical-conferences/epma-2016-1/epma-2016

Conference content preview:
- EPMA, SEM, WDS, EDS techniques
- Tutorial on EPMA and SEM quantitative analysis
- Topics with invited and contributed presentations.

Confirmed invited speakers:
* C. MacRae (Australia - CL and low energy line)
* K. Goemann (Tasmania - shared background by WDS)
* P. Statham (England - state-of-the-art quantitative analysis by EDS)
* X. Llovet (Spain - Monte Carlo simulation, low voltage analysis)
* J. Donovan (OR - Overview of analytical techniques)
* A. Moy (France - Virtual standards)
* E. Vicenzi (MD - Standards and reference materials)
* M.J. Jercinovic (MA - trace element analysis by EPMA)
...and more!
- Discussion and group meeting
- Problems identified by the microanalysis community vs. solutions
provided by the vendor community
- Presentations and product information from the microanalysis vendor community
- Inexpensive housing options
- Group meals

Regards
Heather Lowers, EPMA 2016 ECS Coordinator
Paul Carpenter, EPMA 2016 Chair


Example email which can be used to propose an ECS candidate to EPMA 2016

Send to:
Paul.carpenter.epma-at-gmail.com
hlowers-at-usgs.gov


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Email: m.aindow-at-uconn.edu
Name: Mark Aindow

Organization: University of Connecticut

Title-Subject: [Filtered] Postdoctoral Position in Electron Microscopy

Message: The Institute of Materials Science at UConn is an interdisciplinary center with the
threefold mission of fostering education, research and outreach in all areas of the materials
sciences. The Institute is serving as the temporary home for the UConn-FEI Center for Advanced
Microscopy and Materials Analysis (CAMMA), which includes facilities for scanning and transmission
electron microscopy and focused ion beam instruments (see:
http://www.ims.uconn.edu/uconn-fei-center-of-excellence-in-microscopy-a-tech-park-strategic-partnership/).
CAMMA will move into its permanent home in mid-2017 upon completion of the new Innovation
Partnership Building in the UConn Technology Park.

There is an opening in the Institute for an electron microscopy specialist. The appointee will be
involved in a range of academic and industrial projects, and will assist in the operation of the
Laboratory including: offering short courses, performing routine maintenance, and training/assisting
users of the instruments.
Candidates must hold a PhD in Materials Science or a related discipline and must have extensive
hands-on experience in SEM, FIB and TEM. Experience with electron backscatter diffraction,
aberration-corrected S/TEM, serial-section FIB tomography, and maintenance of instrumentation would
also be beneficial. This is a fixed-term appointment (one-year University Postdoctoral Fellow in the
first instance) and is available from May 1st. Screening of the applications will begin immediately
and will continue until the post is filled. Applications from under-represented groups, including
minorities, women and persons with disabilities are encouraged.

Interested candidates should send a curriculum vitae and the names of at least three referees with
postal addresses, telephone numbers and Email addresses to: Prof. Mark Aindow, Institute of
Materials Science, University of Connecticut, 97 North Eagleville Road, Storrs, CT 06269-3136 USA.
Email: m.aindow-at-uconn.edu


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From: microscopy.listserver-at-gmail.com
Date: March 25, 2016 at 6:16:36 AM CDT
Subject: [Microscopy] Extension of the deadline for abstract submission to Ultrapath XVIII

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Coleagues

The deadline for submitting communications for the Congress Ultrapath
XVIII in Lisbon has been extended one last time to April 23. Do not lose
this opportunity to join your colleagues in an updated overview of
Ultrastructural Pathology.

Your work is very important for the Society and and your contribution to
the Congress will help all of us to keep improving and spread the word
about the continued importance of Ultrastructural Pathology.

With kind regards,

From the Organizing Committee,
A.P. Alves de Matos
Chair of UltrapathXVIII

http://congress.ultrapathXVIII.org


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From: microscopy.listserver-at-gmail.com
Date: March 24, 2016 at 8:14:43 AM CDT
Subject: [Microscopy] viaWWW:high-pressure purifier for carbon dioxide/CPD unit

Contents Retrieved from Microscopy Listserver Archives
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X-from: dlowry-at-asu.edu

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Email: dlowry-at-asu.edu
Name: David Lowry

Organization: Arizona State University

Title-Subject: [Filtered] high-pressure purifier for carbon dioxide/CPD unit

Message: Our critical-point dryer currently uses a Union Carbide model SG-6140 high-pressure
purifier for the carbon dioxide. Our stock of the replacement filters is nearly depleted and UC/Dow
apparently doesnt produce these parts any longer.

Does anyone know of an available cross-reference filter that will fit this UC purifier? Or can
anyone recommend an alternative high-pressure purifier that has readily available parts?

thank you-

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From: microscopy.listserver-at-gmail.com
Date: March 23, 2016 at 10:08:39 PM CDT
Subject: [Microscopy] Conference Proceedings from 1978

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

I am the Publications Officer for the Australian Microscopy and Microanalysis Society and am asking for a little help. I am trying to complete the collection of the Society's publications and am one volume short of a complete collection. The volume I am missing is Book 2 of the Proceedings of the 5th Australian Conference on Electron Microscopy (ACEM) held in Adelaide in 1978.

If you have this volume I am more than willing to pay for postage, scan the volume and send it back to you or if you would prefer to scan it and email it to me that would be wonderful.

The collection at present is a combination of physical and electronic volumes but I am in the process of producing electronic versions of all the publications.

Thank you very much for your assistance with this matter.


Colin Veitch

Manager, Microscopy - Waurn Ponds Laboratory
CSIRO Manufacturing

E colin.veitch-at-csiro.au T +61 3 5246 4891 M 0438 538 475 F +61 3 5246 4057
Address CSIRO Manufacturing, 75 Pigdons Road, Waurn Ponds, Vic 3216, Australia.
www.csiro.au | http://www.csiro.au/Organisation-Structure/Flagships/Manufacturing.aspx


PLEASE NOTE
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From: microscopy.listserver-at-gmail.com
Date: March 23, 2016 at 4:44:13 PM CDT
Subject: [Microscopy] viaWWW:anti-GFP antibody for immunocytochemistry

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Email: jarnikm-at-mail.nih.gov
Name: Michael

Organization: NIH

Title-Subject: [Filtered] anti-GFP antibody for immunocytochemistry

Message: Hi Listers,

I would like to hear tips for a good anti-GFP antibody that would work for
immunocytochemistry (Tokuyasu). I have been using a poyclonal Invitrogene,
but although specific, the sensitivity is rather low. Please feel free to contact me off-list, if
you prefer so.

Thanks, Michael


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From: microscopy.listserver-at-gmail.com
Date: March 23, 2016 at 7:59:42 AM CDT
Subject: [Microscopy] viaWWW: Data logger for FEI Quanta 450

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Did he explain what his goal was in doing this? That would certainly help to know. Indeed, I do not go far in supporting unusual requests unless and until the user is willing to explain themselves. I have had too many occasions where what they wanted to do was actually quite available with another technique that they did not know about.

The Quanta affords a movie option that can be programmed at whatever capture rate they want. Stage X&Y is one of the options for the databar so it could be used to track moves.

I suppose there are some screen capture utilities that could render a movie of the whole screen showing the changes to other parameters. I hope it has a good level of compression. Most of the screen would not be changing from frame to frame and should not take up much of the data stream.

Warren Straszheim
Materials Analysis and Research Lab
Iowa State University

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Email: sbarlow-at-mail.sdsu.edu
Name: Steve Barlow

Organization: SDSU EM Facility

Title-Subject: [Filtered] Data logger for FEI Quanta 450

Message: I have a user who wants to document everything he does while using our FEI Quanta 450 FEGSEM. A call to FEI Tech Support said they can log error messages, but they can't log other actions such as moving sample etc.

My question is, does anyone have an app, or know a company that might sell such an app, that will enable my user to document all his actions on the scope?

Thanks in advance

Steve Barlow
SDSU EM Facility

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From: microscopy.listserver-at-gmail.com
Date: March 22, 2016 at 10:38:14 PM CDT
Subject: [Microscopy] Re: viaWWW: Data logger for FEI Quanta 450

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Hi Steve,

What you are asking for is called "FIB Assist" and was/is made by Fibics
in Ottawa, but I doubt it would be commercially available for Quanta.

For documenting FIB process development work or circuit edits I use
stand-alone video recorder, configured to capture FIB screen at
about three frames per second. Separate PC with Epiphan PCIe is my
favorite solution, but you can get USB-pluggable version from the same
guys or elsewhere:

http://www.epiphan.com/products/compare-pcie-capture-cards/

No any affiliation with either Fibics or Epiphan.

Valery

Valery Ray - also with AIM Lab, UMDCP
=======================================
PBS&T, MEO Engineering Co., Inc.
290 Broadway, Suite 298
Methuen, MA 01844, USA
Phone: +1-978-296-5063
E-Mail: vray-at-partbeamsystech.com
Web: www.partbeamsystech.com
Web: www.freudlabs.com
UMD E-Mail: vray-at-umd.edu

On 3/21/2016 7:46 PM, microscopy.listserver-at-gmail.com wrote:
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Email: sbarlow-at-mail.sdsu.edu
Name: Steve Barlow

Organization: SDSU EM Facility

Title-Subject: [Filtered] Data logger for FEI Quanta 450

Message: I have a user who wants to document everything he does while using our FEI Quanta 450
FEGSEM. A call to FEI Tech Support said they can log error messages, but they can't log other
actions such as moving sample etc.

My question is, does anyone have an app, or know a company that might sell such an app, that will
enable my user to document all his actions on the scope?

Thanks in advance

Steve Barlow
SDSU EM Facility

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From: microscopy.listserver-at-gmail.com
Date: March 21, 2016 at 6:44:30 PM CDT
Subject: [Microscopy] viaWWW: Data logger for FEI Quanta 450

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Hi Charles,

If your alcohol concentrations are correct - as you use them, then I would suspect that the jump from 6.95% to 100% is hydrating the 100% far more than would occur if you used:

1. HCOH 1hr
2. HOH 1hr
3. 70% EtOH 30min
4. 70% EtOH 30min
5. 95% EtOH 30min
6. 95% EtOH 30min
7. 100% EtOH 30min
8. 100% EtOH 30min

My routine - assuming that the number of steps is fixed - dehydrates the tissues far better than the one you're are using, again, if your numbers are correct. Water is almost always the culprit in mechanical processors.

I have NO experience with vacuum processing for light microscopy.

I have stored tissues in 100% EtOH for months in -20C freezer and for years at -80C with no perceptible increase in hardening. 200 Proof EtOH (sorry, ethanol!) becomes 95% EtOH rapidly when dispensed from gallon containers. I used to pour my once-used first and second 100% EtOHs into the first and second 95% containers for the next run. Fresh 100% for every run.

If your Xylene shows any white mist, water is doing it, and miscibility with paraffin will be degraded.

Hope this helps,

Cheers,

Fred Monson

Frederick C. Monson, PhD
Technical Director
Center for Microanalysis and Imaging, Research and Training (CMIRT)
West Chester University of PA
Geology-Astronomy
750 South Church St.
West Chester, PA, 19383
fmonson-at-wcupa.edu
610-738-0437(Work)
Fc.monson-at-gmail.com
610-331-8322 (Mobile-Text)



-----Original Message-----
X-from: Charles Riley via Histonet [mailto:histonet-at-lists.utsouthwestern.edu]
Sent: Tuesday, March 22, 2016 11:27 AM
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Email: sbarlow-at-mail.sdsu.edu
Name: Steve Barlow

Organization: SDSU EM Facility

Title-Subject: [Filtered] Data logger for FEI Quanta 450

Message: I have a user who wants to document everything he does while using our FEI Quanta 450
FEGSEM. A call to FEI Tech Support said they can log error messages, but they can't log other
actions such as moving sample etc.

My question is, does anyone have an app, or know a company that might sell such an app, that will
enable my user to document all his actions on the scope?

Thanks in advance

Steve Barlow
SDSU EM Facility

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From: microscopy.listserver-at-gmail.com
Date: March 21, 2016 at 6:43:36 PM CDT
Subject: [Microscopy] viaWWW: Proteasome staining

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Email: marcela.redigolo-at-mail.wvu.edu
Name: Marcela Redigolo

Organization: WVU

Title-Subject: [Filtered] Proteasome staining

Message: Dear all,

Did anyone work with proteasome samples in TEM? Currently I have a user that brought these samples
in, and we prepared and stained with uranyl acetate. The contrast isn't that great. Before I go
trying other options around, I decided to check if any of you have a better experience with the
sample and a different chemical for staining.

Thanks in advance for all help.

Regards,

Marcela.


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From: microscopy.listserver-at-gmail.com
Date: March 18, 2016 at 2:25:03 PM CDT
Subject: [Microscopy] drying of 3A molecular sieve ( Peter Heimann )

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I keep the alcohols dry with dialysis tubing filled with sieve, and separate tubes of cupric sulfate as an indicator. It will turn blue when water is present. Roughly annually I have to recharge the sieve in the oven at 275C overnight and recharge the CS by heating in a crucible. I usually use a plumbers torch with spread flame- or whenever I run the self-cleaning on the oven I pop last years in. When it turns white it is ready to go. Been using the same material 15 years now.

Scott Whittaker
Manager SEM Lab
Smithsonian Institution
National Museum of Natural History
PO Box 37012 MRC104
Washington DC 20013-7012
202-633-0891

-----Original Message-----
X-from: peter.heimann-at-uni-bielefeld.de [mailto:peter.heimann-at-uni-bielefeld.de]
Sent: Friday, March 18, 2016 8:11 AM
To: Whittaker, Scott {WHITTAKS-at-si.edu}

Dear colleagues,

for drying ("removal of bound water") of 3A-molecular sieve the "web"
recommends at least 300* Celsius ( 572* Fahrenheit resp.).

Would 250* C (482* F) for several hours be sufficient ?

has anybody tried to dry molecular sieve in high vacuum? in microwave oven ?

and has anybody a suggestion for a simple test / indicator if there are traces of water in ethanol or acetone ?

thanks for a short informal reply,

Yours,

Peter

--
====================================================

Dr. Peter Heimann Raum: W7-107 / Tel.: +49-(0)521-106-5628
Universitaet Bielefeld Fax: +49-(0)521-106-5654
"Zellbiologie / Cell Biology" W7-107
33501 Bielefeld, Germanywww.uni-bielefeld.de/biologie/cellbio



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From: microscopy.listserver-at-gmail.com
Date: March 18, 2016 at 10:46:31 AM CDT
Subject: [Microscopy] Looking for technical information for EDAX Apollo X detector

Contents Retrieved from Microscopy Listserver Archives
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Hello,

I would be interested in any technical information/system documentation
for the EDAX Apollo X SDD detector and the EDAX TEAM software.
The detector has a "SIGNAL" output which I suppose outputs the pulses.

How is this signal acquired and processed on the PC ? Is there some kind
of EDAX signal acquisition card ?

Thank you,
Stefan

==============================Original Headers==============================
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From: oshel1pe-at-cmich.edu
Date: March 18, 2016 at 8:33:51 AM CDT
Subject: [Microscopy] Re: drying of 3A molecular sieve ( Peter Heimann )

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Molecular sieve. Indicating sieve can be bought, but it's pricey, so I
mix it 1:4 or 1:5 with non-indicating. I'm using mSorb.

Phil

On 03/18/2016 09:30 , Henk Colijn wrote:
Hi Phil,

Are you thinking of "Drierite" (calcium sulfate) or molecular sieve? I
haven't seen colored molecular sieves.

Cheers,
Henk


--------------------

Hendrik O. Colijn
Center for Electron Microscopy and AnalysiS
The Ohio State University
1305 Kinnear Rd, Suite 100, Columbus, OH 43212

colijn.1-at-osu.edu 614/643-3458
cemas.osu.edu {about:cemas.osu.edu}

"Time is that quality of nature which keeps things from happening all at
once." (Ray Cummings - 1922)
Lately it doesn't seem to be working.



------ Original Message ------


Peter,

I've been using 200 - 250 deg C for "several hours" to dry molecular
sieve for years. Works a treat.
I do make sure to have some molecular sieve that has an indicator dye
mixed in with the regular. It will revert to the "blue = dry" state in
the oven, but it does wear out over time and needs to be refreshed.

No handy test for traces of water, sorry. But, I do keep molecular sieve
in my absolute ethanol. Either lose and handle with great care to not
stir up dust, or in dialysis tubing.

Phil

Dear colleagues,

for drying ("removal of bound water") of 3A-molecular sieve the "web"
recommends at least 300* Celsius ( 572* Fahrenheit resp.).

Would 250* C (482* F) for several hours be sufficient ?

has anybody tried to dry molecular sieve in high vacuum? in microwave
oven ?

and has anybody a suggestion for a simple test / indicator if there are
traces of water in ethanol or acetone ?

thanks for a short informal reply,

Yours,

Peter


--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

==============================Original
Headers==============================
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From: oshel1pe-at-cmich.edu
Date: March 18, 2016 at 8:30:28 AM CDT
Subject: [Microscopy] Re: drying of 3A molecular sieve ( Peter Heimann )

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Phil,

Are you thinking of "Drierite" (calcium sulfate) or molecular sieve? I
haven't seen colored molecular sieves.

Cheers,
Henk


--------------------

Hendrik O. Colijn
Center for Electron Microscopy and AnalysiS
The Ohio State University
1305 Kinnear Rd, Suite 100, Columbus, OH 43212

colijn.1-at-osu.edu 614/643-3458
cemas.osu.edu {about:cemas.osu.edu}

"Time is that quality of nature which keeps things from happening all at
once." (Ray Cummings - 1922)
Lately it doesn't seem to be working.



------ Original Message ------
X-from: oshel1pe-at-cmich.edu
To: colijn.1-at-osu.edu
Sent: 3/18/2016 8:41:30 AM

Peter,

I've been using 200 - 250 deg C for "several hours" to dry molecular
sieve for years. Works a treat.
I do make sure to have some molecular sieve that has an indicator dye
mixed in with the regular. It will revert to the "blue = dry" state in
the oven, but it does wear out over time and needs to be refreshed.

No handy test for traces of water, sorry. But, I do keep molecular
sieve
in my absolute ethanol. Either lose and handle with great care to not
stir up dust, or in dialysis tubing.

Phil

Dear colleagues,

for drying ("removal of bound water") of 3A-molecular sieve the "web"
recommends at least 300* Celsius ( 572* Fahrenheit resp.).

Would 250* C (482* F) for several hours be sufficient ?

has anybody tried to dry molecular sieve in high vacuum? in
microwave
oven ?

and has anybody a suggestion for a simple test / indicator if there
are
traces of water in ethanol or acetone ?

thanks for a short informal reply,

Yours,

Peter


--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

==============================Original
Headers==============================
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From: oshel1pe-at-cmich.edu
Date: March 18, 2016 at 7:39:02 AM CDT
Subject: [Microscopy] Re: drying of 3A molecular sieve ( Peter Heimann )

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Peter,

I've been using 200 - 250 deg C for "several hours" to dry molecular
sieve for years. Works a treat.
I do make sure to have some molecular sieve that has an indicator dye
mixed in with the regular. It will revert to the "blue = dry" state in
the oven, but it does wear out over time and needs to be refreshed.

No handy test for traces of water, sorry. But, I do keep molecular sieve
in my absolute ethanol. Either lose and handle with great care to not
stir up dust, or in dialysis tubing.

Phil

Dear colleagues,

for drying ("removal of bound water") of 3A-molecular sieve the "web"
recommends at least 300* Celsius ( 572* Fahrenheit resp.).

Would 250* C (482* F) for several hours be sufficient ?

has anybody tried to dry molecular sieve in high vacuum? in microwave
oven ?

and has anybody a suggestion for a simple test / indicator if there are
traces of water in ethanol or acetone ?

thanks for a short informal reply,

Yours,

Peter


--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

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From: oshel1pe-at-cmich.edu
Date: March 18, 2016 at 7:07:57 AM CDT
Subject: [Microscopy] drying of 3A molecular sieve ( Peter Heimann )

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Dear colleagues,

for drying ("removal of bound water") of 3A-molecular sieve the "web"
recommends at least 300* Celsius ( 572* Fahrenheit resp.).

Would 250* C (482* F) for several hours be sufficient ?

has anybody tried to dry molecular sieve in high vacuum? in microwave
oven ?

and has anybody a suggestion for a simple test / indicator if there are
traces of water in ethanol or acetone ?

thanks for a short informal reply,

Yours,

Peter

--
====================================================

Dr. Peter Heimann Raum: W7-107 / Tel.: +49-(0)521-106-5628
Universitaet Bielefeld Fax: +49-(0)521-106-5654
"Zellbiologie / Cell Biology" W7-107
33501 Bielefeld, Germanywww.uni-bielefeld.de/biologie/cellbio



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From: microscopy.listserver-at-gmail.com
Date: March 17, 2016 at 9:27:53 PM CDT
Subject: [Microscopy] viaWWW:Overlaying EDS Spectra

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From: microscopy.listserver-at-gmail.com
Date: March 17, 2016 at 9:27:02 PM CDT
Subject: [Microscopy] viaWWW:Re live image to external monitor

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Title-Subject: [Filtered] Re live image to external monitor

Message: Thanks everyone (especially John M) who chimed in with ideas on
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We started out getting the image to the external monitor using VCN
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From: microscopy.listserver-at-gmail.com
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Subject: [Microscopy] viaWWW:Northern California Society for Microscopy Spring Meeting

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From: microscopy.listserver-at-gmail.com
Date: March 17, 2016 at 9:24:58 PM CDT
Subject: [Microscopy] viaWWW:Clinical EM Labs case loads?

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Email: Desertrat99-at-verizon.net
Name: Eric

Organization: UCLA

Title-Subject: [Filtered] Clinical EM Labs case loads?

Message: Hi to the group,

I work in a clinical EM Lab doing mostly renal biopsies. I was
curious to know what other labs do for a workload in term of cases per
year? How many are scoped by the Pathologist and how many are scoped by
the tech?

In our currently there are just me and another tech. Our workload is
approximately 1075 cases. We scope the cases for the pathologists.

Now they want to add 800 cases to our workload to process, cut and scope.

Thanks for your help.

"You only get one sunrise and one sunset a day, and you only get so many
days on the planet. A good photographer does the math and doesnt waste
either." Galen Rowell

"Now this is not the end. It is not even the beginning of the end. But
it is, perhaps, the end of the beginning." Winston Churchill
Eric
The Desert Rat..
This Message made with Recycled Electrons!


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From: microscopy.listserver-at-gmail.com
Date: March 17, 2016 at 7:54:10 PM CDT
Subject: [Microscopy] Postdoc position in STEM, Monash University

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Dear colleagues,

We are advertising a 2-year postdoc position in mapping electromagnetic
fields using electron microscopy (experiment, theory or both) at School
of Physics and Astronomy, Monash University, Australia. Further details
below.

Please bring this position to the attention of anyone who might be
interested.

Many thanks,
Scott Findlay

___________________

Position overview: The Postdoctoral Research Fellow will work on high
resolution transmission electron microscopy imaging of electromagnetic
fields in materials on a project funded by the Australian Research
Council ("Nanoscale field mapping in functional materials").

Duration: Two-year fixed-term appointment

Remuneration package: $68,186 - $92,541 pa Level A / $97,411 - $115,678
pa Level B
(includes 9.50% employer superannuation)

Closing date: Friday 29th April, 2016

Position Descriptions and application details at:
http://www.jobs-monash.jxt.net.au/academic-jobs/postdoctoral-research-fellow-in-electron-microscopy/600759

--
*SCOTT FINDLAY*
QEII Fellow

*School of Physics and Astronomy*
Monash University
G08, New Horizons Centre, Clayton Campus
20 Research Way
Clayton VIC 3800
Australia

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From: microscopy.listserver-at-gmail.com
Date: March 17, 2016 at 7:51:56 AM CDT
Subject: [Microscopy] viaWWW:Biological TEM Workshop at UGA

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Email: jpshield-at-uga.edu
Name: John Shields

Organization: University of Georgia

Title-Subject: [Filtered] Biological TEM Workshop at UGA

Message: Biological TEM Workshop
July 6-8, 2016

This intensive, three-day workshop will provide a practical and basic theoretical introduction to
the Transmission Electron Microscope and biological sample preparation techniques. Each day will
consist of lecture, discussion and hands-on training led by GEM staff.

What: Anyone requiring training on TEM and biological sample preparation. The workshop will be
limited to 6 participants based on the availability of equipment.

When: Wednesday, July 6 through Friday, July 8, 2016, 8am-5pm each day

Where: 154 Barrow Hall, University of Georgia, Athens, GA 30602

Registration: Contact John Shields (jpshield-at-uga.edu) for more information and to sign up.
Registration requires iLab account through the GEM website.
gem.uga.edu


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From: microscopy.listserver-at-gmail.com
Date: March 16, 2016 at 10:05:15 AM CDT
Subject: [Microscopy] viaWWW:Help to measure Area, phase fraction and size

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Email: Prnktmr786-at-gmail.com
Name: Priyanka Tomar

Organization: Student

Title-Subject: [Filtered] Help to measure Area, phase fraction and size

Message: I have a SEM image of four phases nickel, carbon,pores & YSZ. I want to determine
microstructural properties of each phase

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From: microscopy.listserver-at-gmail.com
Date: March 16, 2016 at 10:12:40 AM CDT
Subject: [Microscopy] viaWWW:Blocks of deep-sea fishes

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Name: John McNulty

Organization: Loyola University Chicago

Title-Subject: [Filtered] Blocks of deep-sea fishes

Message: I am retiring and am faced with throwing out epon-embedded tissues from a variety of deep
sea fish species. If anyone would like them, let me know before April 1.
John

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From: microscopy.listserver-at-gmail.com
Date: Thursday, March 10, 2016, 9:03 PM
Subject: [Microscopy] SEM: cleaning used specimen

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Tobias and colleagues,

The only thing I can think of is this one:
glue solubilized in solution, coated the stubs. Insufficient washing (or forgotten because of super bowl this evening?) so enough glue remained on the stubs and insulated them.

As for the economics of cleaning:
Hard to believing that throwing 100 stubs in acetone and wating for the shaker to do its job is worth more than 2 $.
Just don't throw each stub at a time in 100 solutions on 100 shakers and clean them sequentially :-)

Regards
Stephane


--------------------------------------------
On Thu, 3/10/16, baskin-at-bio.umass.edu {baskin-at-bio.umass.edu} wrote:


Colleagues,

A year or two ago, we managed to
make a set of
insulating stubs by cleaning them. Our cleaning procedure
was much as
described in the last few days (razor blade scrabe, followed
by solvent
shaking, followed by grinding). I cannot really fathom how
the stubs
lost conductivity (and batches we cleaned previously were
fine) but lose
it they did. Set us back months troubleshooting that one.
Perhaps a
cautionary tale in addition to the economic argument brought
up below?

As ever,

Tobias
Biology Department, UMass Amherst, MA, 01003, USA

On 3/10/16 2:14 PM, mike_toalson-at-tedpella.com
wrote:

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This is an interesting discussion and a topic we have
discussed a few times
here at Ted Pella, Inc. given that we are manufacturers
and suppliers of
aluminum SEM stubs and Carbon Tabs. So far, our
back of the envelope
calculations say it is not economical to go through
this cleaning given the
cost of labor, chemicals and materials to get them
ready for use again.

Aluminum stubs range in price from around $0.23 EACH
for the small 12.7mm
size --- to $1.60 for the 25mm size --- to as much as
$3-7.00 for more
complex mounts with 45 deg angles or adjustable
surfaces. So spending more
than a few minutes and minimal chemicals/wipes, and it
becomes a losing
battle cost wise. However, that doesn't factor in
your cost to place an
order or shipping. If you don't have a simple
purchasing ability for
supplies and must deal with an onerous system, that
cost in time and labor
could perhaps justify cleaning the carbon adhesive
off.

One also has to factor in the environmental cost of
chemicals and disposal.
Likewise, what is the cost recovery of recycling the
aluminum which is the
more valuable 5000 and 6000 series materials.

We at Ted Pella have considered offering a system of
specialized
solvent/sonicator, grinder or bake-off but the costs
don't seem to justify
cleaning and buying replacements is. But of
course we like selling more
stubs so take all that with a grain of salt. :-)

So, question to you with grad students --- This would
seem like an
interesting paper for the EM community to determine the
economics and best
practice. Ted Pella, Inc. would be willing to
consider funding this
research and publication of results. Please
contact me privately to
discuss.

Mike Toalson
VP Sales & Marketing
Ted Pella, Inc.
PO Box 492477 * Redding, CA 96049
Tel: 530-243-2200 x205 * Cell:
530-356-5921
mike_toalson-at-tedpella.com * www.tedpella.com





-----Original Message-----
X-from: LettJ-at-ent.wustl.edu
[mailto:LettJ-at-ent.wustl.edu]
Sent: Thursday, March 10, 2016 7:15 AM
To: Mike Toalson

----------------------------------------------------------------------------

Does anyone have a good method for cleaning aluminum
specimen mounts so they
can be re-used? Samples were mounted with various
adhesives directly to the
stub surface, as well as with conductive tape.

Thank you,

Jaci

Jaclynn Lett
Senior Research Technician, EM Facility
Research Center for Auditory and Vestibular Studies
Department of Otolaryngology
Washington University School of Medicine
4523 Clayton Avenue, Campus Box 8115
St. Louis, MO 63110
Office: 314-747-7257
Fax: 314-747-7230
Email: lettj-at-ent.wustl.edu
Website: http://otocore.wustl.edu

The Research Center for Auditory and Vestibular Studies
is supported by the
National Institutes of Health NIDCD Grant
P30DC04665. We kindly ask that
all publications and presentations utilizing data
obtained through our
facilities (provided by, prepared by, or imaged using
our equipment) include
a statement acknowledging such.


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From: microscopy.listserver-at-gmail.com
Date: March 12, 2016 at 11:09:17 AM CST
Subject: [Microscopy] viaWWW:Research Assistant Professor at Naval Postgraduate School,

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Email: skmeno1-at-nps.edu
Name: SARATH MENON

Organization: NAVAL POSTGRADUATE SCHOOL

Title-Subject: [Filtered] Research Assistant Professor at Naval Postgraduate School, Monterey, CA

Message:

Research Assistant Professor,
AD-1701-03
Department of Mechanical and Aerospace Engineering
Naval Postgraduate School, Monterey, CA

The Mechanical and Aerospace Engineering Department of the Naval Postgraduate School is inviting
applications from electron Microscopy Specialists to fulfill a Research Faculty position to support
the research and development of a variety of materials including metals, ceramics, carbon-based
materials and composites. The position will be focused on the operation of several advanced
characterization techniques, including fully equipped Scanning Electron Microscope and Transmission
Electron Microscope. Responsibilities of the position include the maintenance and upkeep of these
complex analytical instruments. The applicant should possess operational expertise with SEM, FIB,
TEM lamellae preparation, TEM operation including STEM methods, high resolution TEM and analytical
techniques such as EDS, EBSD, EELS and EFTEM. The candidate for the position must be able to
perform sample analysis with minimal direction and/or oversight from technical principal
investigators. The candidate must also be able to collect, assimilate, and interpret data for
technical principal investigators and write technical reports to document results. The position
description includes also the coordination of all services required to keep instruments operational,
the procurement of all consumables for the microscopy lab and to carry out all the routine
maintenance tasks. A PhD in Materials Science or a closely related field is required. A few years of
hands-on experience with current state-of-the-art instrumentation and techniques in the area of
electron microscopy (SEM, TEM, STEM, EFTEM and FIB) is mandatory. The ability to teach a course
related to Advanced Materials Characterization techniques will be highly valued. In addition, the
candidate is expected to train Masters level students to use instruments and help them with data
analysis. The ability to obtain a US security clearance is mandatory.

Applications should be received by 1 April 2015 and include a curriculum vitae, writing sample,
summary of available teaching evaluations, and three letters of recommendation. Please address the
applications to:

Garth V. Hobson
Professor and Chair
Department of Mechanical & Aerospace Engineering
700 Dyer Road, Bldg 245 Watkins Hall, WA-338
Naval Postgraduate School
Monterey, CA 93943
TEL: 831-656-2586/831-656-2888
www.nps.edu/mae
gvhobson-at-nps.edu



Salary is commensurate with qualifications and experience. Relocation package, including
recruitment/relocation incentive may be authorized. The position will remain open until filled.
The Naval Postgraduate School is an equal opportunity employer. For additional information about
NPS, please refer to the website at http://www.nps.edu.


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From: microscopy.listserver-at-gmail.com
Date: March 12, 2016 at 11:07:52 AM CST
Subject: [Microscopy] viaWWW:PhD scholarship in Electron Microscopic Quantification of

Contents Retrieved from Microscopy Listserver Archives
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X-from: kristian.molhave-at-nanotech.dtu.dk


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Email: kristian.molhave-at-nanotech.dtu.dk
Name: Kristian Molhave

Organization: DTU Nanotech

Title-Subject: [Filtered] PhD scholarship in Electron Microscopic Quantification of Nanoparticle
Aerosols and Condensation behavior

Message:
This project will develop electron microscopy and optical microscopy imaging-based and spectroscopic
methods to establish new ways for aerosol characterization to provide information in high
resolution. Aerosol samples and their changes with time and environmental conditions will also be
observed during in-situ condensation experiments. Such methods will lead to a better understanding
of aerosol sources, their composition and influence of environmental conditions, health effects and
influence on climate.

The position is for three years at the Technical University of Denmark

info on:
http://www.nanotech.dtu.dk/english/About-NTCH/Jobs/job?id=3facbea3-4ecc-43c0-8414-490d9c092b3c

deadline 17th march 2016

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From: microscopy.listserver-at-gmail.com
Date: March 12, 2016 at 11:07:01 AM CST
Subject: [Microscopy] viaWWW:TEM- Gatan Enfinium EELS spectrum energy shift

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Email: fei.long-at-queensu.ca
Name: Fei Long

Title-Subject: [Filtered] TEM- Gatan Enfinium EELS spectrum energy shift

Message: Hello all,

We are encountering an problem with our Gatan Enfinium spectrometer recently. The Autopeels panel
can only capture EELS zero loss peak in the DM software. When we want to shift to higher energy
levels, it simply shift the ZLP to the that energy. Id like to ask if anyone have an idea how I can
solve this problem?
Thanks,


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From: microscopy.listserver-at-gmail.com
Date: March 12, 2016 at 11:06:17 AM CST
Subject: [Microscopy] viaWWW:SEM: cleaning used specimen mounts

Contents Retrieved from Microscopy Listserver Archives
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Email: g.h.ten.brink-at-rug.nl
Name: Gert ten Brink

Organization: RUG/ZIAM

Title-Subject: [Filtered] SEM: cleaning used specimen mounts

Message: Dear Jaci,
I do not even bother to clean them.
They are consumables.
Mind you, if you don't clean the old ones properly
you make your SEM/vacuum dirty.
If you buy them via the internet for a 100 pieces they cost 23.- euro's

The time spent cleaning them cost more.

My 2 cents, Gert

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From: microscopy.listserver-at-gmail.com
Date: March 11, 2016 at 7:37:39 AM CST
Subject: [Microscopy] SEM: cleaning used specimen mounts

Contents Retrieved from Microscopy Listserver Archives
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A great thread!

Working essentially as the only full time person in an SEM lab, long ago I came to the conclusion that unless the cleaning was very quick and easy, it was more practical to simply recycle the small stubs. I could not justify much time to clean them. I do think the larger items are a different story, however. My metallurgical mount holders, vices, multi-stub holders, etc., are pricey and well worth the effort to keep them looking good, both for myself and as well as my clients. For these items, what I can't remove by hand, I ultrasonically clean first with an organic solvent. Once that is done and the parts are dry, I then ultrasonically clean them in a solution of warm water with Alconox powder. I've had surprisingly good success with this. It removes sputtered materials and other residues nicely. The holders come out looking great again with no signs of etching or attack on the aluminum. I've never tried any of the other Alconox products, however.

I have no stake in Alconox.

Chris Holp
Sr. Nuclear Specialist
BETA Labs
Mayfield Village OH 44143
440-604-9704 holpc-at-firstenergycorp.com





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From: microscopy.listserver-at-gmail.com
Date: March 11, 2016 at 5:58:25 AM CST
Subject: [Microscopy] SEM: cleaning used specimen mounts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Working essentially as a one person SEM lab, long ago I came to the conclusion that unless the cleaning was very quick and easy, it was more practical to simply recycle the small stubs. I could not justify much time to clean them. I do think the larger items are a different consideration, however. My metallurgical mount holders, vices, multi-stub holders, etc., are pricey and well worth an effort to keep them looking good, both for myself and clients. For these, I remove paints and tape residues ultrasonically with an organic solvent. Once that is done, I've had decent success by ultrasonically cleaning them in a warm water solution with Alconox powder. This removes sputtered materials and other residuals nicely. The holders come out looking great again with no signs of etching or attack on the aluminum. I've never tried any of the other Alconox products for this, however.

(I have no stake in Alconox.)

Chris Holp
Sr. Nuclear Specialist
BETA Labs
Mayfield Village OH 44143
440-604-9704, holpc-at-firstenergycorp.com




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From: microscopy.listserver-at-gmail.com
Date: March 10, 2016 at 6:36:12 PM CST
Subject: [Microscopy] SEM: cleaning used specimen mounts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,

Just a thought, but for a multiuser facility, it might not make economic
sense for a microscopist to clean them, but it might be a good
facility-level fix to put all the used stubs in a box and make it
available to users with an SOP. That way, anybody needing and lacking
one can grab one from the box and clean it themselves. It takes care of
the labour aspect at least, and is essentially immediate recycling.

-Jake Jokisaari


On 03/10/2016 03:14 PM, oshel1pe-at-cmich.edu wrote:
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We routinely clean off old stubs. Partly because they're easily cleaned
and reused, partly because we have an nice source of free undergrad
labor. Waiting for your next dehydration step? Clean stubs!

The chemical costs aren't great, and the cost-recovery of recycling the
aluminum isn't great either, so cleaning seems best.
Plus, there's no shipping and handling costs for cleaned stubs vs. new ones.

But so far we haven't managed to make insulating stubs ... you sure you
didn't offend Mho, the Spirit of Conductivity, Tobias?

Phil


Colleagues,
A year or two ago, we managed to make a set of
insulating stubs by cleaning them. Our cleaning procedure was much as
described in the last few days (razor blade scrabe, followed by solvent
shaking, followed by grinding). I cannot really fathom how the stubs
lost conductivity (and batches we cleaned previously were fine) but lose
it they did. Set us back months troubleshooting that one. Perhaps a
cautionary tale in addition to the economic argument brought up below?

As ever,
Tobias
Biology Department, UMass Amherst, MA, 01003, USA

On 3/10/16 2:14 PM, mike_toalson-at-tedpella.com wrote:
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This is an interesting discussion and a topic we have discussed a few times
here at Ted Pella, Inc. given that we are manufacturers and suppliers of
aluminum SEM stubs and Carbon Tabs. So far, our back of the envelope
calculations say it is not economical to go through this cleaning given the
cost of labor, chemicals and materials to get them ready for use again.

Aluminum stubs range in price from around $0.23 EACH for the small 12.7mm
size --- to $1.60 for the 25mm size --- to as much as $3-7.00 for more
complex mounts with 45 deg angles or adjustable surfaces. So spending more
than a few minutes and minimal chemicals/wipes, and it becomes a losing
battle cost wise. However, that doesn't factor in your cost to place an
order or shipping. If you don't have a simple purchasing ability for
supplies and must deal with an onerous system, that cost in time and labor
could perhaps justify cleaning the carbon adhesive off.

One also has to factor in the environmental cost of chemicals and disposal.
Likewise, what is the cost recovery of recycling the aluminum which is the
more valuable 5000 and 6000 series materials.

We at Ted Pella have considered offering a system of specialized
solvent/sonicator, grinder or bake-off but the costs don't seem to justify
cleaning and buying replacements is. But of course we like selling more
stubs so take all that with a grain of salt. :-)

So, question to you with grad students --- This would seem like an
interesting paper for the EM community to determine the economics and best
practice. Ted Pella, Inc. would be willing to consider funding this
research and publication of results. Please contact me privately to
discuss.

Mike Toalson
VP Sales& Marketing
Ted Pella, Inc.
PO Box 492477 Redding, CA 96049
Tel: 530-243-2200 x205 Cell: 530-356-5921
mike_toalson-at-tedpella.com www.tedpella.com




-----Original Message-----
X-from: LettJ-at-ent.wustl.edu [mailto:LettJ-at-ent.wustl.edu]
Sent: Thursday, March 10, 2016 7:15 AM
To: Mike Toalson

Does anyone have a good method for cleaning aluminum specimen mounts so they
can be re-used? Samples were mounted with various adhesives directly to the
stub surface, as well as with conductive tape.

Thank you,

Jaci

Jaclynn Lett
Senior Research Technician, EM Facility
Research Center for Auditory and Vestibular Studies
Department of Otolaryngology
Washington University School of Medicine
4523 Clayton Avenue, Campus Box 8115
St. Louis, MO 63110
Office: 314-747-7257
Fax: 314-747-7230
Email: lettj-at-ent.wustl.edu
Website: http://otocore.wustl.edu

The Research Center for Auditory and Vestibular Studies is supported by the
National Institutes of Health NIDCD Grant P30DC04665. We kindly ask that
all publications and presentations utilizing data obtained through our
facilities (provided by, prepared by, or imaged using our equipment) include
a statement acknowledging such.


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notify the sender via telephone or return email.


==============================Original Headers==============================
3, 23 -- From baskin-at-bio.umass.edu Thu Mar 10 13:55:23 2016
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3, 23 -- Thu, 10 Mar 2016 14:55:07 -0500
3, 23 -- Subject: Re: [Microscopy] RE: SEM: cleaning used specimen mounts
3, 23 -- To: mike_toalson-at-tedpella.com, microscnet {microscopy-at-microscopy.com}
3, 23 -- References: {201603101914.u2AJE2hj013333-at-ns.microscopy.com}
3, 23 -- From: Tobias Baskin {baskin-at-bio.umass.edu}
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From: microscopy.listserver-at-gmail.com
Date: March 10, 2016 at 2:55:07 PM CST
Subject: [Microscopy] SEM: cleaning used specimen mounts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We routinely clean off old stubs. Partly because they're easily cleaned
and reused, partly because we have an nice source of free undergrad
labor. Waiting for your next dehydration step? Clean stubs!

The chemical costs aren't great, and the cost-recovery of recycling the
aluminum isn't great either, so cleaning seems best.
Plus, there's no shipping and handling costs for cleaned stubs vs. new ones.

But so far we haven't managed to make insulating stubs ... you sure you
didn't offend Mho, the Spirit of Conductivity, Tobias?

Phil


Colleagues,
A year or two ago, we managed to make a set of
insulating stubs by cleaning them. Our cleaning procedure was much as
described in the last few days (razor blade scrabe, followed by solvent
shaking, followed by grinding). I cannot really fathom how the stubs
lost conductivity (and batches we cleaned previously were fine) but lose
it they did. Set us back months troubleshooting that one. Perhaps a
cautionary tale in addition to the economic argument brought up below?

As ever,
Tobias
Biology Department, UMass Amherst, MA, 01003, USA

On 3/10/16 2:14 PM, mike_toalson-at-tedpella.com wrote:
----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

This is an interesting discussion and a topic we have discussed a few times
here at Ted Pella, Inc. given that we are manufacturers and suppliers of
aluminum SEM stubs and Carbon Tabs. So far, our back of the envelope
calculations say it is not economical to go through this cleaning given the
cost of labor, chemicals and materials to get them ready for use again.

Aluminum stubs range in price from around $0.23 EACH for the small 12.7mm
size --- to $1.60 for the 25mm size --- to as much as $3-7.00 for more
complex mounts with 45 deg angles or adjustable surfaces. So spending more
than a few minutes and minimal chemicals/wipes, and it becomes a losing
battle cost wise. However, that doesn't factor in your cost to place an
order or shipping. If you don't have a simple purchasing ability for
supplies and must deal with an onerous system, that cost in time and labor
could perhaps justify cleaning the carbon adhesive off.

One also has to factor in the environmental cost of chemicals and disposal.
Likewise, what is the cost recovery of recycling the aluminum which is the
more valuable 5000 and 6000 series materials.

We at Ted Pella have considered offering a system of specialized
solvent/sonicator, grinder or bake-off but the costs don't seem to justify
cleaning and buying replacements is. But of course we like selling more
stubs so take all that with a grain of salt. :-)

So, question to you with grad students --- This would seem like an
interesting paper for the EM community to determine the economics and best
practice. Ted Pella, Inc. would be willing to consider funding this
research and publication of results. Please contact me privately to
discuss.

Mike Toalson
VP Sales& Marketing
Ted Pella, Inc.
PO Box 492477 Redding, CA 96049
Tel: 530-243-2200 x205 Cell: 530-356-5921
mike_toalson-at-tedpella.com www.tedpella.com




-----Original Message-----
X-from: LettJ-at-ent.wustl.edu [mailto:LettJ-at-ent.wustl.edu]
Sent: Thursday, March 10, 2016 7:15 AM
To: Mike Toalson

Does anyone have a good method for cleaning aluminum specimen mounts so they
can be re-used? Samples were mounted with various adhesives directly to the
stub surface, as well as with conductive tape.

Thank you,

Jaci

Jaclynn Lett
Senior Research Technician, EM Facility
Research Center for Auditory and Vestibular Studies
Department of Otolaryngology
Washington University School of Medicine
4523 Clayton Avenue, Campus Box 8115
St. Louis, MO 63110
Office: 314-747-7257
Fax: 314-747-7230
Email: lettj-at-ent.wustl.edu
Website: http://otocore.wustl.edu

The Research Center for Auditory and Vestibular Studies is supported by the
National Institutes of Health NIDCD Grant P30DC04665. We kindly ask that
all publications and presentations utilizing data obtained through our
facilities (provided by, prepared by, or imaged using our equipment) include
a statement acknowledging such.


The materials in this email are private and may contain Protected Health
Information. If you are not the intended recipient, be advised that any
unauthorized use, disclosure, copying, distribution or the taking of any
action in reliance on the contents of this information is strictly
prohibited. If you have received this email in error, please immediately
notify the sender via telephone or return email.



==============================Original Headers==============================
3, 23 -- From baskin-at-bio.umass.edu Thu Mar 10 13:55:23 2016
3, 23 -- Received: from marlin.bio.umass.edu (bio.umass.edu [128.119.55.19])
3, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u2AJtNJ0002168
3, 23 -- for {microscopy-at-microscopy.com} ; Thu, 10 Mar 2016 13:55:23 -0600
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3, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES128-SHA bits=128 verify=NO);
3, 23 -- Thu, 10 Mar 2016 14:55:07 -0500
3, 23 -- Subject: Re: [Microscopy] RE: SEM: cleaning used specimen mounts
3, 23 -- To: mike_toalson-at-tedpella.com, microscnet {microscopy-at-microscopy.com}
3, 23 -- References: {201603101914.u2AJE2hj013333-at-ns.microscopy.com}
3, 23 -- From: Tobias Baskin {baskin-at-bio.umass.edu}
3, 23 -- Message-ID: {56E1D11B.1040804-at-bio.umass.edu}
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==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: March 10, 2016 at 2:12:45 PM CST
Subject: [Microscopy] SEM: cleaning used specimen mounts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Long ago, I came to the same conclusion for the stubs. Unless the cleaning was very quick and easy, I recycled the small stubs. I could not justify much time to clean them. I do think the larger items are a different consideration, however. My metallurgical mount holders, vices, multi-stub holders, etc. are well worth it. For these, I remove paints and tape residues ultrasonically with an organic solvent. Once that is done, I've had decent success by ultrasonically cleaning them in a warm water solution with Alconox powder. This removes sputtered materials well enough and seems to be inhibited enough to not attack the aluminum. I've never tried any of the other Alconox products for this, however.

(I have no stake in Alconox.)

Chris Holp
Sr. Nuclear Specialist
BETA Labs
Mayfield Village OH 44143
440-604-9704, holpc-at-firstenergycorp.com

-----Original Message-----
X-from: mike_toalson-at-tedpella.com [mailto:mike_toalson-at-tedpella.com]
Sent: Thursday, March 10, 2016 2:34 PM
To: Holp, Christopher R. {holpc-at-firstenergycorp.com}

This is an interesting discussion and a topic we have discussed a few times here at Ted Pella, Inc. given that we are manufacturers and suppliers of aluminum SEM stubs and Carbon Tabs. So far, our back of the envelope calculations say it is not economical to go through this cleaning given the cost of labor, chemicals and materials to get them ready for use again.

Aluminum stubs range in price from around $0.23 EACH for the small 12.7mm size --- to $1.60 for the 25mm size --- to as much as $3-7.00 for more complex mounts with 45 deg angles or adjustable surfaces. So spending more than a few minutes and minimal chemicals/wipes, and it becomes a losing battle cost wise. However, that doesn't factor in your cost to place an order or shipping. If you don't have a simple purchasing ability for supplies and must deal with an onerous system, that cost in time and labor could perhaps justify cleaning the carbon adhesive off.

One also has to factor in the environmental cost of chemicals and disposal.
Likewise, what is the cost recovery of recycling the aluminum which is the more valuable 5000 and 6000 series materials.

We at Ted Pella have considered offering a system of specialized solvent/sonicator, grinder or bake-off but the costs don't seem to justify cleaning and buying replacements is. But of course we like selling more stubs so take all that with a grain of salt. :-)

So, question to you with grad students --- This would seem like an interesting paper for the EM community to determine the economics and best practice. Ted Pella, Inc. would be willing to consider funding this research and publication of results. Please contact me privately to discuss.

Mike Toalson
VP Sales & Marketing
Ted Pella, Inc.
PO Box 492477 . Redding, CA 96049
Tel: 530-243-2200 x205 . Cell: 530-356-5921 mike_toalson-at-tedpella.com . www.tedpella.com




-----Original Message-----
X-from: LettJ-at-ent.wustl.edu [mailto:LettJ-at-ent.wustl.edu]
Sent: Thursday, March 10, 2016 7:15 AM
To: Mike Toalson

Does anyone have a good method for cleaning aluminum specimen mounts so they
can be re-used? Samples were mounted with various adhesives directly to the
stub surface, as well as with conductive tape.

Thank you,

Jaci

Jaclynn Lett
Senior Research Technician, EM Facility
Research Center for Auditory and Vestibular Studies
Department of Otolaryngology
Washington University School of Medicine
4523 Clayton Avenue, Campus Box 8115
St. Louis, MO 63110
Office: 314-747-7257
Fax: 314-747-7230
Email: lettj-at-ent.wustl.edu
Website: http://otocore.wustl.edu

The Research Center for Auditory and Vestibular Studies is supported by the
National Institutes of Health NIDCD Grant P30DC04665. We kindly ask that
all publications and presentations utilizing data obtained through our
facilities (provided by, prepared by, or imaged using our equipment) include
a statement acknowledging such.


The materials in this email are private and may contain Protected Health
Information. If you are not the intended recipient, be advised that any
unauthorized use, disclosure, copying, distribution or the taking of any
action in reliance on the contents of this information is strictly
prohibited. If you have received this email in error, please immediately
notify the sender via telephone or return email.


==============================Original Headers==============================
8, 35 -- From LettJ-at-ent.wustl.edu Thu Mar 10 08:49:06 2016
8, 35 -- Received: from PCFPRFAGENT02.wusm-pcf.wustl.edu
(pcfprfagent02.wusm-pcf.wustl.edu [128.252.17.171])
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From: microscopy.listserver-at-gmail.com
Date: March 10, 2016 at 1:55:23 PM CST
Subject: [Microscopy] SEM: cleaning used specimen mounts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues,
A year or two ago, we managed to make a set of
insulating stubs by cleaning them. Our cleaning procedure was much as
described in the last few days (razor blade scrabe, followed by solvent
shaking, followed by grinding). I cannot really fathom how the stubs
lost conductivity (and batches we cleaned previously were fine) but lose
it they did. Set us back months troubleshooting that one. Perhaps a
cautionary tale in addition to the economic argument brought up below?

As ever,
Tobias
Biology Department, UMass Amherst, MA, 01003, USA

On 3/10/16 2:14 PM, mike_toalson-at-tedpella.com wrote:
----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

This is an interesting discussion and a topic we have discussed a few times
here at Ted Pella, Inc. given that we are manufacturers and suppliers of
aluminum SEM stubs and Carbon Tabs. So far, our back of the envelope
calculations say it is not economical to go through this cleaning given the
cost of labor, chemicals and materials to get them ready for use again.

Aluminum stubs range in price from around $0.23 EACH for the small 12.7mm
size --- to $1.60 for the 25mm size --- to as much as $3-7.00 for more
complex mounts with 45 deg angles or adjustable surfaces. So spending more
than a few minutes and minimal chemicals/wipes, and it becomes a losing
battle cost wise. However, that doesn't factor in your cost to place an
order or shipping. If you don't have a simple purchasing ability for
supplies and must deal with an onerous system, that cost in time and labor
could perhaps justify cleaning the carbon adhesive off.

One also has to factor in the environmental cost of chemicals and disposal.
Likewise, what is the cost recovery of recycling the aluminum which is the
more valuable 5000 and 6000 series materials.

We at Ted Pella have considered offering a system of specialized
solvent/sonicator, grinder or bake-off but the costs don't seem to justify
cleaning and buying replacements is. But of course we like selling more
stubs so take all that with a grain of salt. :-)

So, question to you with grad students --- This would seem like an
interesting paper for the EM community to determine the economics and best
practice. Ted Pella, Inc. would be willing to consider funding this
research and publication of results. Please contact me privately to
discuss.

Mike Toalson
VP Sales & Marketing
Ted Pella, Inc.
PO Box 492477 Redding, CA 96049
Tel: 530-243-2200 x205 Cell: 530-356-5921
mike_toalson-at-tedpella.com www.tedpella.com




-----Original Message-----
X-from: LettJ-at-ent.wustl.edu [mailto:LettJ-at-ent.wustl.edu]
Sent: Thursday, March 10, 2016 7:15 AM
To: Mike Toalson

Does anyone have a good method for cleaning aluminum specimen mounts so they
can be re-used? Samples were mounted with various adhesives directly to the
stub surface, as well as with conductive tape.

Thank you,

Jaci

Jaclynn Lett
Senior Research Technician, EM Facility
Research Center for Auditory and Vestibular Studies
Department of Otolaryngology
Washington University School of Medicine
4523 Clayton Avenue, Campus Box 8115
St. Louis, MO 63110
Office: 314-747-7257
Fax: 314-747-7230
Email: lettj-at-ent.wustl.edu
Website: http://otocore.wustl.edu

The Research Center for Auditory and Vestibular Studies is supported by the
National Institutes of Health NIDCD Grant P30DC04665. We kindly ask that
all publications and presentations utilizing data obtained through our
facilities (provided by, prepared by, or imaged using our equipment) include
a statement acknowledging such.


The materials in this email are private and may contain Protected Health
Information. If you are not the intended recipient, be advised that any
unauthorized use, disclosure, copying, distribution or the taking of any
action in reliance on the contents of this information is strictly
prohibited. If you have received this email in error, please immediately
notify the sender via telephone or return email.



==============================Original Headers==============================
3, 23 -- From baskin-at-bio.umass.edu Thu Mar 10 13:55:23 2016
3, 23 -- Received: from marlin.bio.umass.edu (bio.umass.edu [128.119.55.19])
3, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u2AJtNJ0002168
3, 23 -- for {microscopy-at-microscopy.com} ; Thu, 10 Mar 2016 13:55:23 -0600
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3, 23 -- (authenticated bits=0)
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3, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES128-SHA bits=128 verify=NO);
3, 23 -- Thu, 10 Mar 2016 14:55:07 -0500
3, 23 -- Subject: Re: [Microscopy] RE: SEM: cleaning used specimen mounts
3, 23 -- To: mike_toalson-at-tedpella.com, microscnet {microscopy-at-microscopy.com}
3, 23 -- References: {201603101914.u2AJE2hj013333-at-ns.microscopy.com}
3, 23 -- From: Tobias Baskin {baskin-at-bio.umass.edu}
3, 23 -- Message-ID: {56E1D11B.1040804-at-bio.umass.edu}
3, 23 -- Date: Thu, 10 Mar 2016 14:55:07 -0500
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3, 23 -- X-Scanned-By: MIMEDefang 2.73
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: March 10, 2016 at 1:13:08 PM CST
Subject: [Microscopy] SEM: cleaning used specimen mounts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This is an interesting discussion and a topic we have discussed a few times
here at Ted Pella, Inc. given that we are manufacturers and suppliers of
aluminum SEM stubs and Carbon Tabs. So far, our back of the envelope
calculations say it is not economical to go through this cleaning given the
cost of labor, chemicals and materials to get them ready for use again.

Aluminum stubs range in price from around $0.23 EACH for the small 12.7mm
size --- to $1.60 for the 25mm size --- to as much as $3-7.00 for more
complex mounts with 45 deg angles or adjustable surfaces. So spending more
than a few minutes and minimal chemicals/wipes, and it becomes a losing
battle cost wise. However, that doesn't factor in your cost to place an
order or shipping. If you don't have a simple purchasing ability for
supplies and must deal with an onerous system, that cost in time and labor
could perhaps justify cleaning the carbon adhesive off.

One also has to factor in the environmental cost of chemicals and disposal.
Likewise, what is the cost recovery of recycling the aluminum which is the
more valuable 5000 and 6000 series materials.

We at Ted Pella have considered offering a system of specialized
solvent/sonicator, grinder or bake-off but the costs don't seem to justify
cleaning and buying replacements is. But of course we like selling more
stubs so take all that with a grain of salt. :-)

So, question to you with grad students --- This would seem like an
interesting paper for the EM community to determine the economics and best
practice. Ted Pella, Inc. would be willing to consider funding this
research and publication of results. Please contact me privately to
discuss.

Mike Toalson
VP Sales & Marketing
Ted Pella, Inc.
PO Box 492477 Redding, CA 96049
Tel: 530-243-2200 x205 Cell: 530-356-5921
mike_toalson-at-tedpella.com www.tedpella.com




-----Original Message-----
X-from: LettJ-at-ent.wustl.edu [mailto:LettJ-at-ent.wustl.edu]
Sent: Thursday, March 10, 2016 7:15 AM
To: Mike Toalson

Does anyone have a good method for cleaning aluminum specimen mounts so they
can be re-used? Samples were mounted with various adhesives directly to the
stub surface, as well as with conductive tape.

Thank you,

Jaci

Jaclynn Lett
Senior Research Technician, EM Facility
Research Center for Auditory and Vestibular Studies
Department of Otolaryngology
Washington University School of Medicine
4523 Clayton Avenue, Campus Box 8115
St. Louis, MO 63110
Office: 314-747-7257
Fax: 314-747-7230
Email: lettj-at-ent.wustl.edu
Website: http://otocore.wustl.edu

The Research Center for Auditory and Vestibular Studies is supported by the
National Institutes of Health NIDCD Grant P30DC04665. We kindly ask that
all publications and presentations utilizing data obtained through our
facilities (provided by, prepared by, or imaged using our equipment) include
a statement acknowledging such.


The materials in this email are private and may contain Protected Health
Information. If you are not the intended recipient, be advised that any
unauthorized use, disclosure, copying, distribution or the taking of any
action in reliance on the contents of this information is strictly
prohibited. If you have received this email in error, please immediately
notify the sender via telephone or return email.


==============================Original Headers==============================
8, 35 -- From LettJ-at-ent.wustl.edu Thu Mar 10 08:49:06 2016
8, 35 -- Received: from PCFPRFAGENT02.wusm-pcf.wustl.edu
(pcfprfagent02.wusm-pcf.wustl.edu [128.252.17.171])
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-0600
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From: microscopy.listserver-at-gmail.com
Date: March 10, 2016 at 12:31:07 PM CST
Subject: [Microscopy] Re: JEOL SEM PC failed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,
I just had the same problem today at a Jeol 5900LV at Munich.
For the communication problem: I suppose your SEM also connects to the
PC via SCSI cable?
If yes, check at the Adaptec SCSI card at Bootup (CTRL+A) if the SCSI ID
is the same like at the back of the SEM console. Adaptec card should
list connect like "JeolSEM ID1" during bootup.
If you get an error during launch of the SEM control program, check for
the SCSI card to be bound to the appropriate "JEOL PCSEM SCSI Processor
Device" driver to be loaded. Otherwise you won`t get a connect to the
SEM. Driver should have come with your installation software by Jeol.
Don`t forget to first switch the SEM on and then checking for the SCSI
problems. ;-)

Best,
Stefan




Am 10.03.2016 um 19:15 schrieb yqin-at-buffalo.edu:
----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Dear Listers,

Recently our JEOL SEM PC motherboard failed, the PC is almost 20 years
old, running Windows 2000. However, the PC still has communication
problems with SEM after replacing the Mother Board. we are not sure
whether the PC has other problems due to the failure of the Mother
Board.

We were wondering whether anybody has a similar situation, and how you
solve this problem? Can we just replace a new PC, and install the
current SEM software to the new PC?

If anybody can help, please contact me.
Thank you!

--



-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.nanoflight.info
www.electronmicroscopy.info
www.elektronenmikroskopie.info
www.assisi.de
www.zwillingsprojekt.de
www.stefan-diller.com
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------


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From: microscopy.listserver-at-gmail.com
Date: March 10, 2016 at 12:06:48 PM CST
Subject: [Microscopy] JEOL SEM PC failed

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers,

Recently our JEOL SEM PC motherboard failed, the PC is almost 20 years
old, running Windows 2000. However, the PC still has communication
problems with SEM after replacing the Mother Board. we are not sure
whether the PC has other problems due to the failure of the Mother
Board.

We were wondering whether anybody has a similar situation, and how you
solve this problem? Can we just replace a new PC, and install the
current SEM software to the new PC?

If anybody can help, please contact me.
Thank you!
--
Dr. Yueling Qin
Senior Research Support Specialist
UB2020 Integrated Nanostructured Systems Initiative,
University at Buffalo, SUNY, Buffalo, NY 14260,
Phone (716) 645-8698

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From: microscopy.listserver-at-gmail.com
Date: March 10, 2016 at 11:39:40 AM CST
Subject: [Microscopy] Re: SEM: cleaning used specimen mounts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

For cleaning large quantities of mounts, shaker is very useful to
minimize, if not eliminate the scrubbing.

Either "real" lab shaker if you have one, or pneumatic type used for
paint mixing. Put used mounts into container (steel, SST, Al, PTFE,
etc...), fill with acetone, put in the shaker, and leave running in fume
hood either overnight or even over the weekend. Soaking for a day prior
to putting in shaker may work better. Or run during the day and switch
off overnight for safety. Can change acetone and shake little more if
lots of carbon tape was used. Rinse with alcohol, dry, and re-use with
virtually no scrubbing.

Valery Ray - also with AIM Lab, UMDCP
=======================================
PBS&T, MEO Engineering Co., Inc.
290 Broadway, Suite 298
Methuen, MA 01844, USA
Phone: +1-978-296-5063
E-Mail: vray-at-partbeamsystech.com
Web: www.partbeamsystech.com
Web: www.freudlabs.com
UMD E-Mail: vray-at-umd.edu

On 3/10/2016 9:52 AM, LettJ-at-ent.wustl.edu wrote:
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Does anyone have a good method for cleaning aluminum specimen mounts so they can be re-used? Samples were mounted with various adhesives directly to the stub surface, as well as with conductive tape.

Thank you,

Jaci

Jaclynn Lett
Senior Research Technician, EM Facility
Research Center for Auditory and Vestibular Studies
Department of Otolaryngology
Washington University School of Medicine
4523 Clayton Avenue, Campus Box 8115
St. Louis, MO 63110
Office: 314-747-7257
Fax: 314-747-7230
Email: lettj-at-ent.wustl.edu
Website: http://otocore.wustl.edu

The Research Center for Auditory and Vestibular Studies is supported by the National Institutes of Health NIDCD Grant P30DC04665. We kindly ask that all publications and presentations utilizing data obtained through our facilities (provided by, prepared by, or imaged using our equipment) include a statement acknowledging such.


The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email.


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From: microscopy.listserver-at-gmail.com
Date: March 10, 2016 at 10:19:11 AM CST
Subject: [Microscopy] Re: SEM: cleaning used specimen mounts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,

Most of the adhesives and paints are soluble in acetone or methanol,
including tape gums, carbon paint, silver paint, and cyanoacrylate
glues. Isopropanol and ethanol can also work, but require more
scrubbing, as it just softens the tape adhesive. I have had success in
cleaning batches of SEM stubs by soaking them in acetone, wiping any
tape off, then sonicating them in acetone or alcohol. Any harder
residues can then be removed with a quick brush over fine sandpaper.

It is a bit time consuming, but it has worked pretty well for me.

Best,

Jake Jokisaari
PhD Graduate
University of Michigan



On 03/10/2016 09:11 AM, LettJ-at-ent.wustl.edu wrote:
----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Does anyone have a good method for cleaning aluminum specimen mounts so they can be re-used? Samples were mounted with various adhesives directly to the stub surface, as well as with conductive tape.

Thank you,

Jaci

Jaclynn Lett
Senior Research Technician, EM Facility
Research Center for Auditory and Vestibular Studies
Department of Otolaryngology
Washington University School of Medicine
4523 Clayton Avenue, Campus Box 8115
St. Louis, MO 63110
Office: 314-747-7257
Fax: 314-747-7230
Email: lettj-at-ent.wustl.edu
Website: http://otocore.wustl.edu

The Research Center for Auditory and Vestibular Studies is supported by the National Institutes of Health NIDCD Grant P30DC04665. We kindly ask that all publications and presentations utilizing data obtained through our facilities (provided by, prepared by, or imaged using our equipment) include a statement acknowledging such.


The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email.


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From: microscopy.listserver-at-gmail.com
Date: March 10, 2016 at 10:17:56 AM CST
Subject: [Microscopy] SEM: cleaning used specimen mounts

Contents Retrieved from Microscopy Listserver Archives
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Hello Everyone,
I scalp the stub with a single edge razor blade to remove as much as possible of the tape and adhesive and then polish the remaining adhesive with on a paper towel with a couple drops of CHCl3. I just place the paper on a table top, drop chloroform on it and holding the stub by the back end and polish the adhesive off. Change spots and replace solvent as needed.

Doing this as soon as possible after removing from the SEM is best. The adhesive and tape seems to harden in air after beam exposure.

-----Original Message-----
X-from: LettJ-at-ent.wustl.edu [mailto:LettJ-at-ent.wustl.edu]
Sent: Thursday, March 10, 2016 10:09 AM
To: Frank Karl

Does anyone have a good method for cleaning aluminum specimen mounts so they can be re-used? Samples were mounted with various adhesives directly to the stub surface, as well as with conductive tape.

Thank you,

Jaci

Jaclynn Lett
Senior Research Technician, EM Facility
Research Center for Auditory and Vestibular Studies
Department of Otolaryngology
Washington University School of Medicine
4523 Clayton Avenue, Campus Box 8115
St. Louis, MO 63110
Office: 314-747-7257
Fax: 314-747-7230
Email: lettj-at-ent.wustl.edu
Website: http://otocore.wustl.edu

The Research Center for Auditory and Vestibular Studies is supported by the National Institutes of Health NIDCD Grant P30DC04665. We kindly ask that all publications and presentations utilizing data obtained through our facilities (provided by, prepared by, or imaged using our equipment) include a statement acknowledging such.


The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email.


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From: microscopy.listserver-at-gmail.com
Date: March 10, 2016 at 9:27:08 AM CST
Subject: [Microscopy] SEM: cleaning used specimen mounts

Contents Retrieved from Microscopy Listserver Archives
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Hi Jaci,

Depending on what the mounting medium was, you may be able to remove it with a solvent, although it will probably still require some elbow grease. Sonicating the mounts may help speed up the process. Conductive tape is removable if you take it off after the SEM examination is complete, but the longer it stays on the mount, the more difficult it becomes to remove.

If you have access to metallography prep equipment, the easiest thing to do is just grind the top of the mount down on an abrasive disc, exposing fresh aluminum. Then, depending on the surface you want, you can leave it with a rough finish, or use finer grits to get a more polished surface.

Hope that helps some!

Jeff
Jeffrey A. Hall | Microscopist
SPI Supplies | 206 Garfield Ave. | West Chester, PA 19380, USA
1-610-436-5400 x106 | jhall-at-2spi.com | http://www.2spi.com
Twitter: http://2spi.com/twitter | Facebook: http://2spi.com/facebook


-----Original Message-----
X-from: LettJ-at-ent.wustl.edu [mailto:LettJ-at-ent.wustl.edu]
Sent: Thursday, March 10, 2016 10:09 AM
To: Jeff Hall {jhall-at-2spi.com}

Does anyone have a good method for cleaning aluminum specimen mounts so they can be re-used? Samples were mounted with various adhesives directly to the stub surface, as well as with conductive tape.

Thank you,

Jaci

Jaclynn Lett
Senior Research Technician, EM Facility
Research Center for Auditory and Vestibular Studies Department of Otolaryngology Washington University School of Medicine
4523 Clayton Avenue, Campus Box 8115
St. Louis, MO 63110
Office: 314-747-7257
Fax: 314-747-7230
Email: lettj-at-ent.wustl.edu
Website: http://otocore.wustl.edu

The Research Center for Auditory and Vestibular Studies is supported by the National Institutes of Health NIDCD Grant P30DC04665. We kindly ask that all publications and presentations utilizing data obtained through our facilities (provided by, prepared by, or imaged using our equipment) include a statement acknowledging such.


The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email.


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From: microscopy.listserver-at-gmail.com
Date: March 10, 2016 at 8:49:07 AM CST
Subject: [Microscopy] SEM: cleaning used specimen mounts

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Does anyone have a good method for cleaning aluminum specimen mounts so they can be re-used? Samples were mounted with various adhesives directly to the stub surface, as well as with conductive tape.

Thank you,

Jaci

Jaclynn Lett
Senior Research Technician, EM Facility
Research Center for Auditory and Vestibular Studies
Department of Otolaryngology
Washington University School of Medicine
4523 Clayton Avenue, Campus Box 8115
St. Louis, MO 63110
Office: 314-747-7257
Fax: 314-747-7230
Email: lettj-at-ent.wustl.edu
Website: http://otocore.wustl.edu

The Research Center for Auditory and Vestibular Studies is supported by the National Institutes of Health NIDCD Grant P30DC04665. We kindly ask that all publications and presentations utilizing data obtained through our facilities (provided by, prepared by, or imaged using our equipment) include a statement acknowledging such.


The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email.


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From: microscopy.listserver-at-gmail.com
Date: March 8, 2016 at 7:17:24 PM CST
Subject: [Microscopy] viaWWW:Free TEM, ultramicrotomes,chiller, freezer, cryostat

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Email: andykloiber-at-yahoo.com
Name: Andrew Kloiber

Organization: Charles River Laboratories - Pathology Associates

Title-Subject: [Filtered] Free TEM, ultramicrotomes,chiller, freezer, cryostat

Message: Hello Listers,
We are in need of office space and have some unused equipment that needs to go.

- Zeiss EM 900 in good working condition except for the trackball mechanism is stuck and the stage
doesnt move in the Y axis.
- Haskris Chiller Model NO. R075S working status unknown.
- Revco Ultima II -80 freezer needs compressors.
-2 Reichert Austria 0m U2 ultramicrotomes.
-Microm HM 505 E Cryostat cutting wheel does not stop.

All of this equipment is available for free pickup at our lab in Durham, NC or you must pay for the
shipping and handling.
Contact me for more information.
andrew.kloiber-at-crl.com

Thanks,
Andy

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From: microscopy.listserver-at-gmail.com
Date: March 8, 2016 at 7:14:11 PM CST
Subject: [Microscopy] viaWWW:Technical Product Support Specialist Position

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Email: rms-at-angstrom.us
Name: Bob Sommerville

Organization: Angstrom Scientific Inc

Title-Subject: [Filtered] Technical Product Support Specialist Position

Message: Technical Product Support Specialist Eastern Region, US

Angstrom Scientific seeks a Technical Product Support Specialist to provide service and hands-on
technical support across our broad portfolio of sophisticated products. This will involve in-house,
field, and remote support via both phone and email.

Angstrom Scientific is a sales, service and distribution company for manufacturers of electron
microscopes, nano-manipulators, advanced specimen holders, and other scientific (capital) equipment
related to nanotech science. We are looking to expand our service support capacity in the eastern US.

Essential Functions / Major Responsibilities:
Install, service, maintain, and train customers at their locations on use of our instruments.
Provide warranty service and post warranty support (i.e. service contracts) at the customer site,
and (via phone and email when appropriate).
Ensure all service calls are documented (start to completion) and Service Reports are properly
completed and submitted in accordance with established procedures.
Maintain own schedule with priorities driven by Service Manager.
Provide tradeshow support and ensure demonstration equipment is prepared and fully functional.
Assist in organizing, maintaining and tracking inventory (spares, consumables, demo equipment) and
take all necessary precautions to safeguard company parts inventory, tools, and equipment.

Specific Job Requirements:
Excellent written and verbal communication skills.
Strong organizational, problem solving, and analytical skills.
Must be detail oriented.
Be flexible to needs of a small office environment.
Ability to understand and organize technical documentation.
Very strong PC and Mac computer skills
Clean DMV record
Drug testing and background check may be required
Approximately 50% travel.

Preferred but not required:
Technical degree (at least associates degree or equivalent)
Hands-on Technical Field service experience
Prior customer interface experience
Experience with troubleshooting and diagnostic methods of scientific equipment.

In compliance with federal law, all persons hired will be required to verify identity and
eligibility to work in the United States and to complete the required employment eligibility
verification document form upon hire

Angstrom Scientific is an equal opportunity employer that does not discriminate on the basis of
race, color, national origin, sex, disability, age, religion, sexual orientation, gender identity,
gender expression, creed, disabled veteran status, marital status, or Vietnam-era veteran status.
Interested Candidates should reply via e-mail to Bob Sommerville at rms -at-angstrom.us

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From: microscopy.listserver-at-gmail.com
Date: March 4, 2016 at 8:23:04 AM CST
Subject: [Microscopy] viaWWW:Research and Development (R&D) Internships in UK

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Email: jhyun-at-gatan.com
Name: John Hyun

Organization: Gatan, Inc.

Title-Subject: [Filtered] Research and Development (R&D) Internships in UK

Message: Role: Two Research and Development (R&D) Internships
Location: Abingdon, Oxfordshire, UK
Duration: 6 months
Internship Description:
Gatan is an industry leader in the electron microscopy industry and the R&D Interns will work as
part of a multidisciplinary team of scientists, engineers and production staff located in the UK.
The roles will provide assistance to technical experts in the delivery of new technology and new
product introductions projects. There will be a requirement for collaboration across all business
functions working closely with the Manufacturing and Customer Service teams to assure the success of
all new product introductions.

The main objective of these internships is learning, personal development, practical application of
their scientific/engineering background as well as familiarizing the individuals with the day to day
challenges of working in an industrial/commercial environment. The prime responsibility of the R&D
Interns will be to engage and take ownership of small projects and product development tasks.
Projects may involve sustaining engineering for mature and legacy products.

The product range and technical challenges are diverse and so the ideal candidates will need broad
scientific knowledge and must be willing to take on new challenges.
The successful candidates will be cable of working semi-autonomously on technical tasks, and will be
comfortable working with existing and proven solutions.
Training and valuable experience will be provided in the following areas:
New product development/introduction (NPD/NPI) procedures and processes
The preparation and release of engineering and other technical documentation within the confines of
a quality management system (QMS)
Engineering change control procedures and product data management (PDM)
The roles will report to the Director of Engineering (UK)
Some UK and international travel may be required.
The following are essential requirements for the internships;
At least a bachelor degree in an applied physical science; a higher postgraduate qualification is
preferred
Fluency in written and oral technical English combined with excellent presentation and general
communication skills
The ability to work collaboratively and cross-culturally
The following skills and experience would be advantageous to the internship;
Experience in the construction, operation and application of electron microscopy technology
Knowledge of software/firmware programming or a high-level language such MATLAB(R)
Knowledge of vacuum technology, cryogenics and thermometry.
Optics and light detection
Electron optics and electron detection
Knowledge of electronics
Familiarity with physics modelling software such as COMSOL(R)
Familiarity with project management tools and techniques

Interested candidates should submit their CV & covering letter to hrus-at-gatan.com


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From: microscopy.listserver-at-gmail.com
Date: March 3, 2016 at 8:39:00 AM CST
Subject: [Microscopy] Philadelphia Society for Microscopy March 10th General Meeting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The Philadelphia Society for Microscopy will hold its first general meeting
of 2016 on Thursday March 10th from 6:30 to 8:30 at Villanova University,
Mendel Hall. There will be a short organization meeting followed by a talk
by Dr. Robert Carlton (retired) on Pharmaceutical Microscopy. For further
information, please visit our Facebook page.



https://www.facebook.com/PhiladelphiaSocietyforMicroscopy/


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From: microscopy.listserver-at-gmail.com
Date: March 2, 2016 at 9:30:57 AM CST
Subject: [Microscopy] SEM with EDS Comparing the new benchtop desktop instruments

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Morning Tom,

I have a 100x100mm stage in a FEI Quanta ESEM. EDS works - with some problems from Oxygen at 15 Torr with water vapor.

If I did not need to serve a large group of users, I could likely do just fine with powders, particles on 12mm circular stubs or even 7-8mm lengths of curtain rod (Older small SEM's from a very reputable manufacturer) if the specimen was constant in dimensions - as in Quality Control .

Service for us is justified by the patterns of use and the criticality of the work that is done. We have service contracts on both the ESEM and the EDS (this is unusual for an EDS). The reason is that faculty in biology, chemistry and geology with laboratories have included individual student contact with the ESEM/EDS tool as part of laboratory schedule. For the EDS the service contract reduces a loss of detector from an 8-week turnaround to 1.

Considering a SEM to be a tool, a careful list of requirements will likely lead to an easy answer to your question.

Everything about a tool on a bench is about compromise. We have a 250 lb. 'Benchtop' X-Ray Diffractometer with a strip-CCD detector that can provide 60K peaks in a 30min scan over 20-125 degrees 2Theta - perfect for student laboratories. (~$100-150k) There is no service contract even though we use it for student laboratories. In the XRD everything inside is modular: computer, controller and goniometer, source and detector.

We also have a 250 lb. benchtop vacuum coater that can't be put on a bench against a wall, because 75% of its maintenance is done thru the rear of the unit. For our uses - no contract. Cleaning and servicing the TMP is relatively easy. The control system is modular and can easily be diagnosed over the phone and countered by exchange by overnight mail and screw driver and cable changes.

What I have seen of the small SEM with EDS is that data are the same - % by weight and atomic % + a couple others estimates. Currently, my only REAL consideration would be whether the benchtop I purchased had a SDD detector instead of the Si(Li) that all of us users want to replace with one.

Admittedly long, though I hope it helps,

Fred Monson

Frederick C. Monson, PhD
Technical Director
Center for Microanalysis and Imaging, Research and Training (CMIRT)
West Chester University of PA
Geology-Astronomy
750 South Church St.
West Chester, PA, 19383
fmonson-at-wcupa.edu
610-738-0437(Work)



-----Original Message-----
X-from: tkremer-at-ipstesting.com [mailto:tkremer-at-ipstesting.com]
Sent: Wednesday, February 17, 2016 10:56 AM
To: Monson, Frederick {FMonson-at-wcupa.edu}

Hello All,

Do any of you have experience with the newer benchtop or desktop SEM's? They seem quite capable and their cost to purchase and maintain, compared to the full scale SEM's, looks attractive. Their advertised performance is appealing. Do they maintain that performance? Do they require much service? Should I have a service contract? Do the accessories function well (e.g. rotating and tilting stage/holder, charge reduction holder)? Is the provided EDS system comprehensive and reliable? Your replies would be greatly appreciated.

Tom Kremer
tkremer-at-ipstesting.com


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From: microscopy.listserver-at-gmail.com
Date: February 29, 2016 at 8:15:42 PM CST
Subject: [Microscopy] Re: Retirement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear John

You been one of the pillars of the community. We are sorry to see
you go and hope to still see you occasionally post a wise word or two.

Enjoy retirement!

Barbara Foster, President & Chief Consultant
Microscopy/Microscopy Education ... "Education, not Training"
7101 Royal Glen Trail, Suite A - McKinney, TX 75070 - P: 972-924-5310
www.MicroscopyEducation.com

Microscopy/Microscopy Education is a division of The Microscopy &
Imaging Place, Inc.


NEW! Getting involved in Raman or FTIR?
MME is now offering courses in these areas specifically for microscopists!
Now scheduling courses through the mid 2016. We can customize a
course on nearly any topic, from fluorescence to confocal to image
analysis to SEM/TEM.
Call today for a free training evaluation.



At 05:44 PM 2/29/2016, you wrote:



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Tomorrow is my last day at Arizona State University. I am retiring
after 44 years of microscopy. Well, we all know that electron
microscopes are pretty cool, so my work years have been fun, and all
the people around the microscopes are of a similar mind-set, so that
has made the work cool also. Thanks to Professor Will Bigelow and
Dr. Larry Allard at the University of Michigan for introducing me to
this world and training me to do well in it, thanks to Professor Ray
Carpenter for recruiting me to ASU, and thanks to Nestor Zaluzec for
maintaining the Listserver for all these years. Well, I must have
been fated to work in the field, because in the year I was born:

. JEOL was founded as the Japan Electron Optics Laboratory Company,
Limited, Tokyo.
. Philips First production Transmission Electron Microscope (TEM)
system was released
. Raimond Castaing showed the first functioning microprobe.
. C.W. Oatley built an SEM based on Zworykin's microscope.
. First microscopic analysis of thin metal foils and viewing
dislocations was accomplished by Robert Heidenreich.

For anyone else who I did not mention explicitly (and there are too
many to list), thanks also.

Nestor, do not disconnect me, because retirees from this institution
retain their email accounts, so even though I won't be battling
traffic to get to the lab, I will still read postings.

A. John Mardinly, Ph.D., P.E.
Principal Materials Nanoanalysis Engineer
EMail: John.Mardinly-at-ASU.edu
John Cowley Center for HREM, LE-CSSS
B134B Bateman Physical Sciences Building
Arizona State University
PO Box 871704
Tempe, AZ 85287-1704



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From: microscopy.listserver-at-gmail.com
Date: February 29, 2016 at 5:24:16 PM CST
Subject: [Microscopy] Retirement

Contents Retrieved from Microscopy Listserver Archives
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Tomorrow is my last day at Arizona State University. I am retiring after 44 years of microscopy. Well, we all know that electron microscopes are pretty cool, so my work years have been fun, and all the people around the microscopes are of a similar mind-set, so that has made the work cool also. Thanks to Professor Will Bigelow and Dr. Larry Allard at the University of Michigan for introducing me to this world and training me to do well in it, thanks to Professor Ray Carpenter for recruiting me to ASU, and thanks to Nestor Zaluzec for maintaining the Listserver for all these years. Well, I must have been fated to work in the field, because in the year I was born:

. JEOL was founded as the Japan Electron Optics Laboratory Company, Limited, Tokyo.
. Philips First production Transmission Electron Microscope (TEM) system was released
. Raimond Castaing showed the first functioning microprobe.
. C.W. Oatley built an SEM based on Zworykin's microscope.
. First microscopic analysis of thin metal foils and viewing dislocations was accomplished by Robert Heidenreich.

For anyone else who I did not mention explicitly (and there are too many to list), thanks also.

Nestor, do not disconnect me, because retirees from this institution retain their email accounts, so even though I won't be battling traffic to get to the lab, I will still read postings.

A. John Mardinly, Ph.D., P.E.
Principal Materials Nanoanalysis Engineer
EMail: John.Mardinly-at-ASU.edu
John Cowley Center for HREM, LE-CSSS
B134B Bateman Physical Sciences Building
Arizona State University
PO Box 871704
Tempe, AZ 85287-1704



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From: microscopy.listserver-at-gmail.com
Date: Thu, 12 May 2016 20:15:54 -0500
Subject: [Microscopy] Administrivia: Listserver Archives back working

Contents Retrieved from Microscopy Listserver Archives
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Colleagues....

Thanks to the heads up from Lucille Giannuzzi
a problem with the Listserver archives which
was inadvertently created is now fixed.

Basically the problem was that messages were not being
stored due to a mistake on my part which affected
all of March, April and the the first 2 weeks of May.

The archives are now restored/fixed
and the missing messages have been reinserted into
the archive file. The only glitch is that when I inserted
the missing messages into the archive, the temporal order is backwards
for the restored messages.

This means if you happen to scan/dump the archives you will
see messages in the temporal sequential order of:
Jan, Feb, May(1-12), April, March, then back to May (13-} ).

Fixing the exact order is alot of work as I would
have to manually cut and paste messages into the
correct order. I'll put that off for now.

Nestor
Your Friendly Neighborhood SysOp


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From: gary-at-gaugler.com
Date: Thu, 12 May 2016 23:10:31 -0500
Subject: [Microscopy] EDS detector resolution checking

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings Listers:

Have any of you checked the resolution of your EDS detector to compare
it's resolution with what you bought versus what you have?

I discovered that the Mn FWHM is a known standard method, but the EDS
makers don't necessarily actually use this. It seems that they use a
radioactive Fe specimen and then correlate that to Mn. I'm wondering if
the correlation/translation from Fe to Mn is valid. My current system is
not.

The situation could be that you bought a 123eV detector but wound up
with one that is greater than 123eV. The delivered spec might say
{=123eV yet the resolution is actually worse than this.

Any feedback or comments?

gary g.

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From: jrminter-at-gmail.com
Date: Fri, 13 May 2016 17:44:23 -0500
Subject: [Microscopy] Re: EDS Detector Resolution

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I bought a small standard block from Ted Pella that has Mn as one of the standards. It also has a BN standard useful for checking low end performance. I record a spectrum from the standard using my standard conditions, save it in MSA format, and then use the NIST-DTSA-II to process the spectrum. One first needs to configure a DTSA detector and then run the detector calibration “alien". It will calibrate from the Mn. The routine automatically computes the resolution.

It turns out that one can do a pretty good job with a piece of Cu tape or a Cu TEM grid mounted on an SEM stub. If one measures the Cu spectrum at 25 kV the intensity of the Cu K and L lines are pretty close. One can then run the detector calibration from the Cu spectrum. The results on our system are within experimental error (which DTSA automatically computes!) of the results from Mn. What else would you expect from Nicholas Ritchie and his colleagues at NIST!

I have no commercial interest in Ted Pella; just a long time satisfied customer. Also a grateful user of DTSA-II.

Best regards,
John Minter
Kodak Analytical Sciences

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From: rmott-at-pulsetor.com
Date: Sat, 14 May 2016 11:19:38 -0500
Subject: [Microscopy] Re: EDS detector resolution checking

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Gary Gaugler wrote:

} Have any of you checked the resolution of your EDS detector
} to compare it's resolution with what you bought versus what
} you have?
}
} I discovered that the Mn FWHM is a known standard method, but
} the EDS makers don't necessarily actually use this. It seems
} that they use a radioactive Fe specimen and then correlate
} that to Mn. I'm wondering if the correlation/translation from
} Fe to Mn is valid. My current system is not.
}
} The situation could be that you bought a 123eV detector but
} wound up with one that is greater than 123eV. The delivered
} spec might say {=123eV yet the resolution is actually worse
} than this.
}

Dear Gary --

I'm one of the guilty parties, going back about 4 decades.

First, the emission of an Fe55 radioactive source really is the Mn K
lines.
It is identical to the K emission of Mn under the beam, except there is
no
Brehmsstrahlung background. Instead of ionization by an electron beam,
the excited Mn is created by electron capture from an Fe55 nucleus
(nuclear
proton grabs an electron and becomes a neutron, reducing the atomic
number
by 1). It is convenient and very safe, because no other radiation is
emitted (*).
Sources up to 100 uCuries can be sent by regular mail with no special
packaging.

Detectors are normally tested in a controlled environment "on the bench"
with a source. There are many reasons a detector might not meet spec on
a column in the real world besides the detector itself. For example, CE
marking
be d*mned, we've discovered that one of our Tektronix digital
oscilloscopes
costs about 1.5 eV if it's anywhere near the BNC cables from the
detector to
the pulse processor. X-ray detectors are exquisitely sensitive EMI
detectors
too. You might find for that resolution varies slightly with the time of
day,
depending on what other equipment in the area is operating. I've spent
many happy (?) hours at various installations trying to figure out what
was
causing resolution degradation. 123 eV is close to the absolute limit
for
a silicon-based detector, and it only takes microvolts of noise to kick
that
up an eV or so.

Anybody else have enough gray in their hair to remember when anything
better than 150 eV was a pretty decent detector?

I second the kudos to Nicholas Ritchie! DTSA-II is an extraordinarily
useful tool for all aspects of EDS analysis. Good luck figuring out
what's
going on.

Rick Mott, PulseTor LLC (formerly with PGT back when
woolly mammoths still walked the hills of New Jersey)


(*) Ok, all you picky nuclear physicists out there. There are extremely
low probability gammas up to 231 KeV, 5 to 7 orders of magnitude lower
in intensity than the Mn K lines. Not worth losing sleep over.



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From: dcristofori-at-unive.it
Date: Mon, 16 May 2016 12:14:22 -0500
Subject: [Microscopy] TEM-EDS: looking for Oxford ISIS Series 300 spare parts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listservers
it seems we're in big troubles with the EDS of our TEM. We have an old
Oxford ISIS Series 300, and it isn't collecting signal any longer. It
seems it's the XAC card, which has been discontinued by Oxford,
unfortunately.
I fear that we won't be able to gather the money for a new system, so it
would be great if some of you had an old ISIS - or just the XAC card -
no longer in use, to give us.
Thanks

Davide


~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~
Davide Cristofori

direct phone = 041.234.67.26
e-mail = dcristofori[at]unive[dot]it

Universit Ca' Foscari Venezia
Dipartimento di Sc. Molecolari e Nanosistemi
Centro di Microscopia Elettronica "Giovanni Stevanato"

Campus Scientifico, Edificio ETA
Via Torino 155 I-30172 Mestre (VE) Italy
~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~

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From: microscopy.listserver-at-gmail.com
Date: Tue, 17 May 2016 07:16:56 -0500
Subject: [Microscopy] viaWWW:Scientist position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: baxau-at-mail.nih.gov

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
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Email: baxau-at-mail.nih.gov Name: Ulrich Baxa

Organization: Frederick National Laboratory for Cancer Research

Title-Subject: [Filtered] Scientist position

Message: Dear Colleagues,

The Electron Microscopy Laboratory at the Frederick National Laboratory for Cancer Research, located
in Frederick, Maryland, has an open position of Scientist I. Main duties will include: 1) management
of a small user facility with sample processing lab and FEI T12 microscope, 2) biological sample
preparation for electron microscopy (traditional plastic embedding, high pressure freezing/freeze
substitution, CLEM, other approaches as necessary), 3) help users with routine imaging and training
of new users. If interested, please apply directly using the following link:

http://jobs.leidos.com/ShowJob/Id/831561/Scientist-I-%28Electron-Microscopy%29-628259-%28NCI%29/

Best regards,

Ulrich Baxa


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From: xinran.liu-at-yale.edu
Date: Tue, 17 May 2016 07:18:59 -0500
Subject: [Microscopy] Yale 2016 Microscopy Workshop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues,

We would like to draw your attention to the upcoming Yale Microscopy Workshop, a 2-day event that offers open access to a wide variety of microscope.

Yale Microscopy Workshop
June 14-15th, 2016

This year's symposia theme is “Novel Fluorescent Probes and Labeling Techniques". We will also offer a small group practical micro-course on “Spectral detection and spectra unmixing". As usual, there are a very broad range of technical lectures and demonstrations that are too numerous to mention here, but include a practical lecture on sample preparation for Correlative Light and EM. There will also be vendor presentations on commercially available probes to highlight cell structures or function.

Because this year’s symposia topic is focused on probes and labeling, there will not be any additional microscopes installed by vendors. Instead we will continue to take advantage of some of the excellent microscopes in facilities on campus, including two Leica confocals with spectral detectors in the CCMI facility, one of them with STED superresolution capabilities.

Something for everyone, for beginners as well as the advanced microscopists! Please see the website for a complete listing of events: http://medicine.yale.edu/lab/microscopy/schedule.

Organizers:
Ann Haberman
Derek Toomre
Joerg Bewersdorf
Xinran Liu





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From: jehrman-at-mta.ca
Date: Wed, 18 May 2016 12:30:56 -0500
Subject: [Microscopy] objective swap?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Listers,

With this year's spring cleaning and microscope consolidation, I've
ended up with a what I believe to be a brand new (or nearly so; I can't
see any flaws) 63X/1.25 Zeiss Plan-Neofluar oil immersion objective. I
more or less have these coming out of my ears, but am short a 20X
objective. If anybody has a spare and gently used 20X/0.50 Zeiss
Plan-Neofluar I'd happily lose magnification, numerical aperture and
capital value in a straight swap.

Contact me off-list if interested.

Thanks,
Jim

--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
63B York St.
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/dmf

By the time they had diminished
from 50 to eight, the other dwarves
began to suspect "Hungry".


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From: microscopy.listserver-at-gmail.com
Date: Wed, 18 May 2016 18:52:33 -0500
Subject: [Microscopy] viaWWW:TEM service rep

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Title-Subject: [Filtered] TEM service

Message: Does any know of an independent TEM service rep or tech that offers service contracts?

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From: microscopy.listserver-at-gmail.com
Date: Wed, 18 May 2016 18:53:20 -0500
Subject: [Microscopy] viaWWW:Tenure Track Position at Naval Postgraduate School

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Email: skmeno1-at-nps.edu Name: SARATH MENON

Organization: NAVAL POSTGRADUATE SCHOOL

Title-Subject: [Filtered] Tenure Track Position at Naval Postgraduate School

Message: TENURE-TRACK ASSISTANT PROFESSOR – Material Science
The department of Mechanical and Aerospace Engineering (MAE) at the Naval
Postgraduate School, in Monterey California, is seeking outstanding candidates for a
tenure-track Assistant Professor position starting September 2016. The candidate should
have a Ph.D. in Mechanical Engineering or in Material Science/Engineering.
Demonstrated expertise in structural materials and advance materials characterization is
required, preferentially with an emphasis on metal joining and corrosion. New faculty
are expected to develop high quality externally-funded research programs and teach at the
graduate levels. The candidate will have an exciting opportunity to collaborate with
faculty in the Center for Materials Research that includes the Departments of MAE,
Physics and Electrical and Computer Engineering. Facilities within MAE include a new
Transmission Electron Microscope, a Scanning Electron Microscope, as well as a new
Metallic Cold Spray facility, among others.
Review of applicants will begin April 1, 2016. The Naval Postgraduate School is
committed to enhancing the diversity of its faculty and staff and encourages applications
from women, minorities, people with disabilities and veterans. As a tenure-track faculty
member the applicant must be able to obtain a security clearance. The Naval Postgraduate
School offers a competitive salary, start-up package, and benefits. Please visit our
department website at http://www.nps.edu/Academics/GSEAS/MAE/

Garth V. Hobson
Professor and Chair
Department of Mechanical & Aerospace Engineering
700 Dyer Road, Bldg 245 – Watkins Hall, WA-338
Naval Postgraduate School
Monterey, CA 93943
TEL: 831-656-2586/831-656-2888
FAX: 831-656-2238
www.nps.edu/mae
gvhobson-at-nps.edu

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From: mdelann1-at-jhmi.edu
Date: Thu, 19 May 2016 09:08:52 -0500
Subject: [Microscopy] viaWWW:TEM service rep

Contents Retrieved from Microscopy Listserver Archives
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Michael,
I have info on an independent contratctor (Remi) that will service
microscopes. We have not used him, but here is the contact email:

X-from: Tim Johnson [mailto:Tim.Johnson-at-theremigroup.com]
Sent: Wednesday, April 06, 2016 9:26 AM
To: 'mdelann1-at-jhmi.edu'

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Email: michael.pidgeon-at-uhsystem.com Name: Michael Pidgeon

Organization: University Health / Louisiana State University

Title-Subject: [Filtered] TEM service

Message: Does any know of an independent TEM service rep or tech that offers
service contracts?

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From: microscopy.listserver-at-gmail.com
Date: Thu, 19 May 2016 20:17:46 -0500
Subject: [Microscopy] viaWWW:Light microscope recommendations

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Email: bethrichardson-at-uga.edu Name: Beth Richardson

Organization: University of Georgia

Title-Subject: [Filtered] Light microscope recommendations

Message: Hi all,
I need recommendations for a basic light microscope with a camera system. Let me know if you have a
system in your lab that you love (Leica, Zeiss, Olympus or another company). thanks,
Beth Richardson
Georgia Electron Microscopy Lab
University of Georgia
Athens, GA 30602 USA


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From: microscopy.listserver-at-gmail.com
Date: Thu, 19 May 2016 20:18:55 -0500
Subject: [Microscopy] viaWWW:SEM free to good home

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Email: rgmorse-at-morse-associates.com Name: Roger G Morse

Organization: Morse Associates

Title-Subject: [Filtered] SEM free to good home

Message: I have an old (1970's vintage) Cambridge Stereoscan 600 that I would like to give to
somebody who could use it. It last worked about 12 years ago, and is complete. I expect it could
be very useful for parts for anybody that is trying to keep an old Stereoscan working.

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From: stefan.diller-at-t-online.de
Date: Fri, 20 May 2016 09:17:19 -0500
Subject: [Microscopy] Jeol 6500 FESEM manuals

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Dear All,
can anybody help me with an operation manual and a manual documenting the API of the remote control on the Jeol 6500 FESEM in PDF?

Thanks,
Stefan

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From: John.Mardinly-at-asu.edu
Date: Fri, 20 May 2016 13:04:31 -0500
Subject: [Microscopy] Attention American Post-Docs: New Federal Overtime Rules!

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Starting December 1st, slave labor will be illegal:

www.nytimes.com/2016/05/18/business/white-house-increases-overtime-eligibility-by-millions.html


A. John Mardinly, Ph.D., P.E.
Retired from ASU







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From: microscopy.listserver-at-gmail.com
Date: Wed, 25 May 2016 07:54:42 -0500
Subject: [Microscopy] viaWWW:

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Email: kunihiro.uryu-at-rockefeller.edu Name: Kunihiro Uryu

Organization: Rockefller University, EMRC

Title-Subject: [Filtered] Tannic acid staining for microtubule in brain

Message: Dear list,

I am experiencing sub-optimal microtubule staining in ependymal cells in mouse brain tissue by
tannic acid, while the same protocol works fine on microtubules in cultured cells. I use 0.1% of
tannic acid in 2.5% glutaraldehyde for 1 hour. I extended the incubation time to overnight for the
brain experiments but it was still selective and weak. Any advice is appreciated.

Regards,
Hiro
-----------
Kunihiro Uryu, Ph.D., Research Assistant Professor
Director of Electron Microscopy Resource Center (EMRC)

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From: microscopy.listserver-at-gmail.com
Date: Thu, 26 May 2016 07:51:01 -0500
Subject: [Microscopy] viaWWW:M&M 2016 Pre-Meeting Congress - TEM and SEM Diffraction

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Email: ypicard-at-cmu.edu Name: Yoosuf Picard

Organization: Carnegie Mellon University

Title-Subject: [Filtered] M&M 2016 Pre-Meeting Congress - TEM and SEM Diffraction Workshop

Message: EVENT: Pre-Meeting Congress on ““Exploiting the Diffractive Properties of Electrons for
Solving Materials Problems”

http://www.microscopy.org/MandM/2016/program/congress.cfm
DATE: Sunday, July 24, 2016 preceding the Microscopy and Microanalysis 2016 meeting

LOCATION: Columbus, Ohio, USA at M&M 2016

1 Day Workshop: 8 In-Depth Tutorials featuring Practical Advice and Surveying Various Case Studies
/ Latest Research Highlights on SEM and TEM Diffraction-Based Methods

REGISTRATION: $149 for MAS/MSA/IMS members; $50 for students
Link to register for M&M 2016 and this event:
https://ww2.eventrebels.com/er/Registration/StepRegInfo.jsp?ActivityID=15715&StepNumber=1


Link if you’ve already registered for M&M 2016:
https://ww2.eventrebels.com/er/Registration/UpdateInfo.jsp?ActivityID=15715

PROGRAM: Marc De Graef, Carnegie Mellon University - Introduction to crystallography
and electron diffraction

John Mansfield, University of Michigan - Conventional TEM Diffraction Methods for Materials Analysis
and Crystallography

Joe Michael, Sandia National Laboratories - Introduction and Survey of Electron Backscattered
Diffraction

Ute Kolb, University of Mainz - Precession Diffraction for TEM-based Orientation Mapping

Ben Britton, Imperial College - Strain Mapping by HR-EBSD

Jiong Zhang, Intel - TEM-based Strain Mapping Techniques

Michael Mills, Ohio State University - Imaging Defects by Diffraction Contrast in the TEM

Yoosuf Picard, Carnegie Mellon University - Non-destructive Defect Imaging by in the SEM

SPONSORS: FEI / NanoMEGAS / Hitachi / Zeiss / Gatan E.A. Fischione Instruments /
JEOL / Tescan / EDAX / Oxford Instruments

PURPOSE OF EVENT:
Diffraction is a fundamental mechanism for structural characterization of materials by both
transmission electron microscopes (TEMs) and scanning electron microscopes (SEMs). For new users,
diffraction methods in TEM and SEM are often taught in separate courses or workshops even though the
underlying physics and materials applications overlap substantially. Also, there rarely exist
educational opportunities that singularly cover the myriad options for TEM/SEM based
characterization of crystal structure, orientation, strain and defects where electron diffraction is
central.

To address this concern, a Pre-Meeting Congress entitled “Exploiting the Diffractive Properties of
Electrons for Solving Materials Problems” will be held Sunday, July 24, 2016 in Columbus, OH, USA.
This one-day meeting is being organized by us, the Electron Crystallography & Automated Mapping
Techniques (ECAMT) Focused Interest Group (FIG), within the purview of the Microscopy Society of
America (MSA). This one day event precedes the Microscopy and Microanalysis 2016 meeting held this
coming July 25-28 in Columbus, OH.

This Pre-Meeting Congress reviews basic methodologies in the analysis of crystalline materials using
electron diffraction (specific speakers and topics can be found listed above and in the attached
flyer). Both SEM and TEM methods are featured. This Congress will feature eight invited speakers,
each an internationally renowned expert in the utilization of electron diffraction methods for
analyzing one or more of the following structural properties of crystalline materials:
phase/symmetry, orientation, defects, and strain. Each speaker will describe the fundamental
physics, explain the basic methodologies and approaches, and highlight key recent research findings
and/or important technique developments. This session provides an excellent opportunity for electron
microscopists to review SEM and TEM methodologies based on electron diffraction, and gain new
insights on the latest advances and applications of state-of-the-art diffraction methods. This
workshop is also extremely beneficial to students and researchers new to SEM/TEM.

Registration coincides with registration for the Microscopy and Microanalysis 2016 meeting at the
link provided above. We look forward to your presence at this event and we greatly acknowledge and
appreciate financial support from our sponsors.

ORGANIZERS:
Microscopy Society of America (MSA) Focused Interest Group (FIG) on Electron Crystallography and
Automated Mapping Techniques (ECAMT)

Jörg Wiezorek, University of Pittsburgh Yoosuf Picard, Carnegie Mellon University
Sergei Rouvimov, University of Notre Dame
Robert Stroud, nanoMegas




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From: microscopy.listserver-at-gmail.com
Date: Fri, 27 May 2016 07:02:36 -0500
Subject: [Microscopy] viaWWW:TEM- Looking for best particle counting software?

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Email: skeller-at-tem-analysis.com
Name: Sandra Keller
Organization: TA-SICCO

Title-Subject: [Filtered] TEM- Looking for best particle counting software?

Message: Hi:
I am looking for the best software option(s) for counting and sizing particles in a TEM micrograph.
In my previous experiences, I found most software that I had experimented with to be cumbersome and
unreliable at distinguishing particles, especially ones with low contrast. Any recommendations of
software that users have found to be reliable and easy to use would be most welcome.
SK

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From: eikonika-at-otenet.gr
Date: Fri, 27 May 2016 13:40:14 -0500
Subject: [Microscopy] Emitech K550X sputter coater issue

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Hello Dear List
We have a problem with our Emitech K550X sputter coater (version2.1 -fully
digitized): The argon inlet valve remains constantly open and vacuum cannot
establish. We can still coat if we keep the argon regulator closed from the
cylinder, so that no argon enters the chamber.
Can the problem be something simple or there is an electronic fault behind?
If anybody has any suggestions please advice.
Best regards
yorgos

Dr Yorgos Nikas
Athens Innovative Microscopy
Skra 36 Voula 16673 GREECE

Tel/fax +30 210 8957677
mobile +30 6945 107477
www.eikonika.net www.aim.cat
*************************************







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7, 20 -- From: "Yorgos Nikas" {eikonika-at-otenet.gr}
7, 20 -- To: {microscopy-at-microscopy.com}
7, 20 -- Subject: Emitech K550X sputter coater issue
7, 20 -- Date: Fri, 27 May 2016 21:40:16 +0300
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From: jerry.biehler-at-gmail.com
Date: Fri, 27 May 2016 14:27:30 -0500
Subject: [Microscopy] Manual for Commonwealth IBS-250

Contents Retrieved from Microscopy Listserver Archives
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I know this is a long shot, but does anyone out there have a manual for a Commonwealth Scientific IBS-250 or 600 Ion Beam Power supply? My usual sources have drawn a blank and Veeco has not gotten back to me.

-Jerry

==============================Original Headers==============================
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From: eikonika-at-otenet.gr
Date: Fri, 27 May 2016 15:52:48 -0500
Subject: [Microscopy] Emitech K550X sputter coater issue

Contents Retrieved from Microscopy Listserver Archives
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We managed to fix the problem by hammering (gently) the metal valve that was
probably stuck
Thanks to everybody who replied
yorgos

-----Original Message-----
X-from: eikonika-at-otenet.gr [mailto:eikonika-at-otenet.gr]
Sent: Friday, May 27, 2016 9:53 PM
To: eikonika-at-otenet.gr

Hello Dear List
We have a problem with our Emitech K550X sputter coater (version2.1 -fully
digitized): The argon inlet valve remains constantly open and vacuum cannot
establish. We can still coat if we keep the argon regulator closed from the
cylinder, so that no argon enters the chamber.
Can the problem be something simple or there is an electronic fault behind?
If anybody has any suggestions please advice.
Best regards
yorgos

Dr Yorgos Nikas
Athens Innovative Microscopy
Skra 36 Voula 16673 GREECE

Tel/fax +30 210 8957677
mobile +30 6945 107477
www.eikonika.net www.aim.cat
*************************************







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15, 20 -- To: {microscopy-at-microscopy.com}
15, 20 -- Subject: Emitech K550X sputter coater issue -FIXED
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From: microscopy.listserver-at-gmail.com
Date: Sun, 29 May 2016 15:17:50 -0500
Subject: [Microscopy] viaWWW:EVO SEM STAGE IS NOT INITIALISED

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Email: dsmrc.bulletin-at-gmail.com
Name: Sithu Tun

Organization: myanmar medical research

Title-Subject: [Filtered] SEM STAGE IS NOT INITIALISED

Message: My evo ls 10 zeiss sem has a problem of stage is not initialised.
please kindly answer me how to solve this.
With respect


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From: microscopy.listserver-at-gmail.com
Date: Mon, 30 May 2016 14:07:03 -0500
Subject: [Microscopy] viaWWW:FIB micropillar methods

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Email: 13qw9-at-queensu.ca Name: Qiang

Organization: Queen's University

Title-Subject: [Filtered] FIB micropillar methods

Message: Dear all, Are there anybody can provide a good recipe for FIB
micropillars? My circular pillar ends up with a ditch surrounding the
bottom, which makes it difficult to measure the exact height of the
pillar. Thank you!

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From: Nicolas.Stephant-at-univ-nantes.fr
Date: Tue, 31 May 2016 03:59:21 -0500
Subject: [Microscopy] Plasma cleaner XEI

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Dear listers

I would like to know if the plasma of XEI Evactron system is generated
by induction or not.

Thanks

--
Nicolas STEPHANT

Université de Nantes
Institut Jean Rouxel
Service de microscopie électronique à balayage et microanalyse
2 rue de la Houssinière
BP 92208
44322 Nantes cédex 3

Tél : 02 40 37 64 26
Mél : nicolas.stephant-at-univ-nantes.fr
Web : http://cnrs-imn.fr

"Le monde n'existe que pour autant que nous sommes capables d'en produire une image"
C.G Jung


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8, 24 -- From: Nicolas Stephant {Nicolas.Stephant-at-univ-nantes.fr}
8, 24 -- Subject: Plasma cleaner XEI
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==============================End of - Headers==============================




From: microscopy.listserver-at-gmail.com
Date: Tue, 31 May 2016 20:06:14 -0500
Subject: [Microscopy] viaWWW:

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Email: rms-at-angstrom.us Name: Bob Sommerville

Organization: Angstrom Scientific Inc

Title-Subject: [Filtered] Technical Sales Position Available
Message: Position: Technical Sales Representative – Mid-Western US
Position Description: This is an exciting and established territory with primary emphasis on selling
and supporting the Hitachi TM series of table-top scanning electron microscopes. In addition,
Angstrom Scientific Inc. represents a number of leading companies in the electron microscopy and
related markets. The ideal candidate will preferably have a demonstrated track record of success (
2-3 years) selling technical products into the academic, research and industrial markets. Optical
microscopy or SEM/TEM related experience is a definite plus. Academic background should be at min an
associate’s degree in science (BS or masters preferred) The successful candidate will live in OH MI
or IL. Angstrom Scientific Inc is a growing distributor of products targeted to the nanotechnology,
research and semiconductor markets with exciting new and novel technology Angstrom Scientific Inc
offers competitive salary and benefits, commission, car allowance and expenses. We are an equal
opportunity employer.
Interested candidates should submit their resume together with salary history to: Bob Sommerville,
General Sales Manager, Angstrom Scientific Inc.
e-mail: rms-at-angstrom.us

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From: microscopy.listserver-at-gmail.com
Date: Wed, 1 Jun 2016 06:53:49 -0500
Subject: [Microscopy] viaWWW:Research associate position at Univ. of Delaware microscopy

Contents Retrieved from Microscopy Listserver Archives
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X-from: cni-at-udel.edu

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Email: cni-at-udel.edu Name: Chaoying Ni

Organization: University of Delaware

Title-Subject: [Filtered] Research associate position at microscopy center

Message: Research Associate - Materials Science and Engineering (W.M. Keck Center for Advanced
Microscopy and Microanalysis, http://www.camm.udel.edu)

Pay Grade: 28E

Deadline: June 18, 2016

Under limited supervision, performs a variety of complex technical laboratory duties including the
design and construction of test equipment. Supervises and trains personnel. The principal emphasis
is on working independently and exercising personal initiative in conducting experiments using
complex electron and light microscopes, scanning probe microscopes and associated sample preparation
equipment and experiment devices.

MAJOR RESPONSIBILITIES:

* Performs a variety of technical duties involved in conducting electron microscopic
characterization and analysis to obtain data in support of research activities.
* Performs contract work or experimental consultation to support industrial collaborations.
* Sets up instrumentation; calibrates equipment and repairs equipment problems.
* Procures and maintains material stock.
* Helps to set and maintain proper laboratory safety standards.
* Develops and modifies experimental procedures.
* Trains (written and oral) graduate students and post-doctors for using electron microscopes and
related equipment.
* Prepares tables, graphs and report.
* Performs other related duties as assigned.

QUALIFICATIONS:

Requires a minimum of a Bachelor's degree and one year of experience in laboratory work in a
relevant field as well as experience with the necessary physical, electronic and chemical systems.
Supervisory experience preferred.
Related progressive experience beyond a high school diploma or GED may be substituted for required
education or additional related education may be substituted for required experience. Requires
knowledge of laboratory techniques, procedures and instrumentation;

Requires abilities to read and interpret complex operations manuals, drawings and design, interpret
problems and design equipment for the particular tasks, assume responsibility and take initiative to
perform tasks, work independently, train and instruct others, and communicate effectively and
interact well with people of all ages and diverse backgrounds.

OCCUPATIONAL EXPOSURES:

May be required to use personal protective equipment to prevent exposure to hazardous materials.

How To Apply


https://udjobs.nss.udel.edu:4450/psp/RESUME/EMPLOYEE/HRMS/c/HRS_HRAM.HRS_CE.GBL?Page=HRS_CE_JOB_DTL&Action=A&SiteId=1&JobOpeningId=103665&PostingSeq=1


When applying please submit a one-page cover letter and your resume as one document. Also, please
remember to provide names, addresses and telephone number of at least three references in the online
application.

Equal Employment Opportunity

Employment offers will be conditioned upon successful completion of a criminal background check. A
conviction will not necessarily exclude you from employment.

The University of Delaware is an Equal Opportunity Employer which encourages applications from
Minority Group Members, Women, Individuals with Disabilities and Veterans. The University's Notice
of Non-Discrimination can be found at http://www.udel.edu/aboutus/legalnotices.html



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From: klivi-at-jhu.edu
Date: Wed, 1 Jun 2016 12:06:40 -0500
Subject: [Microscopy] Job Opportunity

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am listing this Job Opportunity for a friend.

Please Respond to mzhu6-at-uwyo.edu NOT to me.
-------------------------------------------------------------------------------------------------

Postdoctoral Research Associate in Environmental Mineralogy

The Soil and Environmental Biogeochemistry Group in the Department of Ecosystem Science and Management at the University of Wyoming, Laramie, WY, USA is seeking candidates for a postdoctoral position in Environmental Mineralogy to work on mineral formation using in situ liquid cell transmission electron microscopy (LC-TEM). The 3-year project is funded by the U.S. Department of Energy (FY2016 – FY2019), which will start on August 1, 2016. The starting annual salary is $40,000 with full benefits with possible raises each year.

Manganese i(Mn) oxides are versatile minerals and have both economic and environmental significance, but little is known regarding their formation mechanisms. This project will determine nucleation and particle growth mechanisms of layered and tunneled manganese (Mn) oxides using cutting-edge in situ liquid cell transmission electron microscopy (LC‑TEM). Synchrotron X-ray techniques, such as X-ray atomic pair distribution function (PDF) analysis, will also be used.

Requirement for consideration of applications includes:
1) A Ph.D. degree in appropriate areas of Mineralogy, Materials Science, Geochemistry, Chemistry, or a closely related field.
2) Proficient in operating high-resolution TEM (HR-TEM) instruments and have extensive experience in HR-TEM characterization of inorganic materials.
3) LC-TEM experience is not required, but preferred.
4) Work independently.
5) Excellent written and verbal communication skills in English
6) Candidates must be legally able to work in the United States

This is a collaborative project with a staff scientist with expertise on LC-TEM analysis at the Pacific Northwest National Laboratory, Richland, WA. The major portion of the research will be conducted at the University of Wyoming. The post‑doc will be trained at PNNL for LC-TEM analysis. The post-doc will have opportunities to attend academic conferences, teach or give guest lectures for a Mineralogy course, and mentor graduate students in the group.

Applicants should submit a cover letter, curriculum vita, and names and contact information for at least 3 referees who can provide a letter of recommendation upon request, to Dr. Mengqiang (Mike) Zhu at mzhu6-at-uwyo.edu. In the cover letter, applicants need to state research interests and experience addressing the criteria listed above and give a date of availability. All documents must be submitted as a single pdf file. The application review will start immediately and continues until the position is filled. Anticipated project start date is August 1, 2016, but later start dates for the successful candidate can be considered. For more information regarding the position, please contact Dr. Mengqiang Zhu (307-766-5523, mzhu6-at-uwyo.edu).



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From: tina-at-pbrc.hawaii.edu
Date: Wed, 1 Jun 2016 22:19:40 -0500
Subject: [Microscopy] Instruction manual for Sorvall Glass Knife Maker

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have inherited an old Sorvall glass knife maker that we would like to
use (modify, i fnecessary) for scoring and breaking 2mm thick glass into
one inch squares. No one remembers how to use it... Do any of you have a
manual for the thing?

Aloha,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************

==============================Original Headers==============================
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From: wij.muss-at-aon.at
Date: Thu, 2 Jun 2016 10:21:55 -0500
Subject: [Microscopy] Re: Instruction manual for Sorvall Glass Knife Maker

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

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Forwarded from "Ask a Microscopist"
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I have already suggested ImageJ, but perhaps the list knows of other good programs for doing this.

Name:
Piragash Kumar R M
School:
Manipal Institute of Technology
Grade/Education Level:
Graduate
Location:
Manipal,
IN
Email:
prakashkumar03-at-gmail.com

Dear Tina,

hopefully you got a lot of answers (??) .

Would it be possible to tell which one of all those found by Google (cf.
https://www.google.com/search?q=sorvall+glass+knife+maker) you really mean?
(since - if I remember correctly - also the former LKB - or later on - the
Leica Knife Maker sometimes were called { Sorvall....} )...

For the LEICA EM KMR2, the REICHERT,LKB-2nd Generation as well as for the
LKB knife breaker_7800B_, 7800, 7801, 2178 I do have manuals in my files....

But if ist really the "SORVALL Glass Knife Maker" as found at:
http://microtomeserviceco.com/index.php?route=product/product&product_id=57
you might get info or perhaps also a copy of the original Manual via:

4072 Rusty Pine Lane Liverpool NY 13090 U.S.A. 315-451-1404
Mitch-at-MicrotomeServiceCo.com
(Disclaimer: no affiliation with, nor any financial interest)

Unfortunately I can't help with an original manual but perhaps -at-
http://facpub.stjohns.edu/~trombetl/lab%20manual.htm you can find sufficing
information at least for a try....*)
Best wishes, good luck ,
cordially yours,
Wolfgang

Wolfgang MUSS
Retired (still member of MSA?)
SALZBURG, AUSTRIA

* PS:
SORVALL GLASS KNIFE MAKER - INSTRUCTIONS

I - Wash glass rectangles with alconox and scrub brush.

II - Reducing glass rectangles to 2.54cm (1 in) squares.

1 - Remove plastic cover.

2 - Center glass using a ruler on the top plate against the back stop plate.
Support ends of glass rectangle if the rectangle is very long otherwise
excessive pressure will be placed on the score line.

3 - Check glass to ensure that the manufacturer's score is down (This side
is identified by minute ridges along the edge)

4 - Turn the handwheel clockwise while holding the glass in place. After the
bumpers have made contact with the glass, complete on more full turn of the
handwheel.

The glass should be held securely. Try to rock the anvil to
see if the glass is secure. If the anvil can be moved, continue turning the
hand wheel RAPIDLY until the anvil is tight.

5 - Pull the plunger adjustment shaft out to prepare for the short stroke.

6 - Pull the scoring shaft all the way out. Depress the button and push the
scoring shaft back with one even stroke to score the glass.

7 - SLOWLY, GENTLY, and CAREFULLY turn the breaking control knob clockwise
until the glass breaks.

8 - Turn the handwheel counterclockwise to raise the clamp and anvil
NEVER TURN THE BREAK CONTROL KNOB
COUNTERCLOCKWISE IT WILL RESET AUTOMATICALLY
NEVER RAISE THE CLAMP ARM TO ITS HIGHEST
POSITION

9 - Remove glass by placing your fingers on top of the glass and sliding
the pieces toward you.

Repeat all steps until glass is broken into 2.54 cm squares.

_______________________________________________________
III - Preparation of Knives

1 - Inspect the corners and sides of the square carefully. Select the
corner that is the smoothest ( with the fewest score marks) and the most
nearly perpendicular to the 2,54cm square.

2 - Place the glass square on the top plate with the selected corner seated
in the back stop plate registration notch. The scored edges of the glass
square should now be up

3 - Push the positioner slide lightly against the corner of the glass
facing the operator.

4 - Hold the positioner slide lightly in place while turning the
handwheel clockwise to clamp the glass to the top plate.

5 - Push the plunger adjustment shaft IN for a long stroke.

6 - Pull the positioner back so it is flush against the top plate surface.

7 - Pull the scoring shaft all the way out. Depress the button and push the
scoring shaft back in one even stroke.

8 - Place plastic cover over the instrument.

9 - SLOWLY AND CAREFULLY turn the breaking control knob CLOCKWISE until the
glass breaks.

10 - Remove the plastic cover.

11 - Turn the handwheel counterclockwise to raise the clamp arm and anvil.
NEVER TURN THE BREAK CONTROL KNOB COUNTERCLOCKWISEIT WILL
RESET AUTOMATICALLY
NEVER RAISE THE CLAMP ARM TO ITS HIGHEST POSITION

Inspect each glass knife

The outer one-third of the knife edge that parallels the stress line will
be the sharpest section.

The final test of the acceptability of the glass knife is to examine the
knife edge for "whiskers". This is done under a binocular stereo microscope
with bright field illumination from above against a dark background.


-----Ursprngliche Nachricht-----
Von: tina-at-pbrc.hawaii.edu [mailto:tina-at-pbrc.hawaii.edu]
Gesendet: Donnerstag, 02. Juni 2016 05:44
An: wij.muss-at-aon.at
Betreff: [Microscopy] Instruction manual for Sorvall Glass Knife Maker




----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

I have inherited an old Sorvall glass knife maker that we would like to use
(modify, i fnecessary) for scoring and breaking 2mm thick glass into one
inch squares. No one remembers how to use it... Do any of you have a manual
for the thing?

Aloha,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************

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From: joki-at-umich.edu
Date: Thu, 2 Jun 2016 12:05:39 -0500
Subject: [Microscopy] Re: Ask a Microscopist: Tools for "dot" counting in

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,

This is actually an interesting question, as I am curious what people
generally use for image analysis. It seems like there is a bewildering
array of free software, manufacturer supplied software, and third-party
software out there. The methods used by a lot of these software
packages seem pretty black-box.

In terms of free software, I second the suggestion of ImageJ; it works
well with regular optical micrographs. Another option is to look to the
maker of the microscope you are using, I think that most of the makers
provide at least rudimentary particle analysis software with the
equipment. Nikon provides a package called 'NIS-Elements'
(http://www.nikon.com/products/microscope-solutions/lineup/img_soft/),
though it is not free.

Apart from that, there are other programs that are optimized for EM or
AFM that might work, such as gwyddion (free), SPIP (not free), or with
an EM focus, Digital Micrograph (from Gatan, for EM but takes any
greyscale image and I believe there is a free-as-in-beer demo version
available)

Other than those options, a quick google search turned up several third
party commercial image analysis packages that claim to be able to do
particle analysis. I have not looked at any of these in depth, so I
cannot make any recommendations for these.

-Jake Jokisaari



On 06/02/2016 08:15 AM, oshel1pe-at-cmich.edu wrote:
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} to the person asking the question.
} Please copy their email address from their question.
} *************************************************************************************
}
} I have already suggested ImageJ, but perhaps the list knows of other good programs for doing this.
}
} Name:
} Piragash Kumar R M
} School:
} Manipal Institute of Technology
} Grade/Education Level:
} Graduate
} Location:
} Manipal,
} IN
} Email:
} prakashkumar03-at-gmail.com
} Subject:
} Counting dots in an image; microscopy
} Your Question:
} I would like to know about the available methods and tools to count the number of dots in an optical microscope image and thus find the percentage area occupied by the dots in the image. I work with Nikon Eclipse optical microscope and the images are captured at 1000x magnification using Clemex Captiva image acquisition platform (Clemex CCD camera). Please help me and thanks in advance
}
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From: tina-at-pbrc.hawaii.edu
Date: Thu, 2 Jun 2016 13:17:18 -0500
Subject: [Microscopy] Found - Re: Instruction manual for Sorvall Glass Knife Maker

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi, Everyone-

Thanks for all the replies which, as usual, asked even more questions. Wai
Pang Chan sent a link to the full manual for the Sorvall/DuPont glass
knife maker:

http://depts.washington.edu/if/files/sorvall_gkm_manual.pdf

On the third page down there's a picture of the thing, for those who
asked.

Now to see if we can make it break 2mm glass, because I've never been able
to mod the LKB to do it.

Aloha,
Tina

} I have inherited an old Sorvall glass knife maker that we would like to use
} (modify, i fnecessary) for scoring and breaking 2mm thick glass into one
} inch squares. No one remembers how to use it... Do any of you have a manual
} for the thing?
}
} Aloha,
} Tina
}
} ****************************************************************************
} * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu
} *
} * Biological Electron Microscope Facility * (808) 956-6251
} *
} * University of Hawaii at Manoa *
} http://www.pbrc.hawaii.edu/bemf*
} ****************************************************************************
}

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************

==============================Original Headers==============================
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From: LettJ-at-ent.wustl.edu
Date: Thu, 2 Jun 2016 13:52:23 -0500
Subject: [Microscopy] RE: Sorvall Glass Knife Maker

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We have one of these, but the sueded leather disc inside the handwheel assembly slips such that the glass is not held tightly enough against the anvil. Does anyone have a do-it-yourself solution?

Thank you,

Jaci

Jaclynn Lett
Senior Research Technician, EM Facility
Research Center for Auditory and Vestibular Studies Department of Otolaryngology Washington University School of Medicine
4523 Clayton Avenue, Campus Box 8115
St. Louis, MO 63110
Office: 314-747-7257
Fax: 314-747-7230
Email: lettj-at-ent.wustl.edu
Website: http://otocore.wustl.edu

The Research Center for Auditory and Vestibular Studies is supported by the National Institutes of Health NIDCD Grant P30DC04665. We kindly ask that all publications and presentations utilizing data obtained through our facilities (provided by, prepared by, or imaged using our equipment) include a statement acknowledging such.

----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver

Hi, Everyone-

Thanks for all the replies which, as usual, asked even more questions. Wai Pang Chan sent a link to the full manual for the Sorvall/DuPont glass knife maker:

http://depts.washington.edu/if/files/sorvall_gkm_manual.pdf

On the third page down there's a picture of the thing, for those who asked.

Now to see if we can make it break 2mm glass, because I've never been able to mod the LKB to do it.

Aloha,
Tina

} I have inherited an old Sorvall glass knife maker that we would like
} to use (modify, i fnecessary) for scoring and breaking 2mm thick glass
} into one inch squares. No one remembers how to use it... Do any of you
} have a manual for the thing?
}
} Aloha,
} Tina
}
} ****************************************************************************
} * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu
} *
} * Biological Electron Microscope Facility * (808) 956-6251
} *
} * University of Hawaii at Manoa *
} http://www.pbrc.hawaii.edu/bemf*
} **********************************************************************
} ******
}

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************

==============================Original Headers==============================
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==============================Original Headers==============================
18, 35 -- From LettJ-at-ent.wustl.edu Thu Jun 2 13:52:22 2016
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From: thoward-at-unm.edu
Date: Thu, 2 Jun 2016 15:51:31 -0500
Subject: [Microscopy] RE: Sorvall Glass Knife Maker

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Jaci -

You can buy little suede-ish, stick-on sewing thimbles:

https://www.missouriquiltco.com/shop/detail/27551/colonial-needle-company/-/thimblepad-leather-adhesive-thimble

Would one of those (cut down) work?

No financial interest blah blah blah - but the Missouri shop seemed appropriate for Jaci.

Tamara

...................................................
Tamara Howard
Dept. of Cell Biology & Physiology
University of New Mexico
Albuquerque, NM


________________________________________
X-from: LettJ-at-ent.wustl.edu {LettJ-at-ent.wustl.edu}
Sent: Thursday, June 02, 2016 12:54 PM
To: Tamara Howard

We have one of these, but the sueded leather disc inside the handwheel assembly slips such that the glass is not held tightly enough against the anvil. Does anyone have a do-it-yourself solution?

Thank you,

Jaci

Jaclynn Lett
Senior Research Technician, EM Facility
Research Center for Auditory and Vestibular Studies Department of Otolaryngology Washington University School of Medicine
4523 Clayton Avenue, Campus Box 8115
St. Louis, MO 63110
Office: 314-747-7257
Fax: 314-747-7230
Email: lettj-at-ent.wustl.edu
Website: http://otocore.wustl.edu

The Research Center for Auditory and Vestibular Studies is supported by the National Institutes of Health NIDCD Grant P30DC04665. We kindly ask that all publications and presentations utilizing data obtained through our facilities (provided by, prepared by, or imaged using our equipment) include a statement acknowledging such.

----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver

Hi, Everyone-

Thanks for all the replies which, as usual, asked even more questions. Wai Pang Chan sent a link to the full manual for the Sorvall/DuPont glass knife maker:

http://depts.washington.edu/if/files/sorvall_gkm_manual.pdf

On the third page down there's a picture of the thing, for those who asked.

Now to see if we can make it break 2mm glass, because I've never been able to mod the LKB to do it.

Aloha,
Tina

} I have inherited an old Sorvall glass knife maker that we would like
} to use (modify, i fnecessary) for scoring and breaking 2mm thick glass
} into one inch squares. No one remembers how to use it... Do any of you
} have a manual for the thing?
}
} Aloha,
} Tina
}
} ****************************************************************************
} * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu
} *
} * Biological Electron Microscope Facility * (808) 956-6251
} *
} * University of Hawaii at Manoa *
} http://www.pbrc.hawaii.edu/bemf*
} **********************************************************************
} ******
}

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************

==============================Original Headers==============================
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From: Rosemary.White-at-csiro.au
Date: Thu, 2 Jun 2016 17:16:43 -0500
Subject: [Microscopy] Ask a Microscopist: Tools for "dot" counting in

Contents Retrieved from Microscopy Listserver Archives
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Another place to look, though it’s aimed more at plant biologists, is http://www.plant-image-analysis.org/, although it’s assumed that most users are familiar with ImageJ, to some extent. The point-detection plugins are pretty easy in ImageJ.

Is there a similar directory of image analysis packages for other applications? My feeling would be, probably not, as it’d be enormous…

cheers,
Rosemary

Dr Rosemary White
CSIRO Black Mountain
GPO Box 1600
Canberra, ACT 2601
Australia
Adjunct Prof, EH Graham Centre, CSU & Research School of Biology, ANU

T 61 2 6246 5475
E rosemary.white-at-csiro.au









On 3/06/2016 3:17 am, "joki-at-umich.edu" {joki-at-umich.edu} wrote:

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From: dev.c0debabe-at-gmail.com
Date: Tue, 7 Jun 2016 07:00:02 -0500
Subject: [Microscopy] Looking for manual for IDE Active Vibration Isolation System

Contents Retrieved from Microscopy Listserver Archives
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Hi,

we recently obtained a second-hand Active Vibration Isolation system
made by Integrated Dynamics Engineering (IDE).
The system comes with a "MAXCON-1000" controller which is connected to
the plate with 4 motor and 4 isolator cables.
We think that the system also has transport protection clamps that need
to be removed before use, but unfortunately we don't have a manual for
the system.

If you have a manual, software or any other information for this system
it would be great if you could send it to me.

Thanks you,
Stefan

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From: microscopy.listserver-at-gmail.com
Date: Tue, 7 Jun 2016 07:53:36 -0500
Subject: [Microscopy] viaWWW:Scanning transmission electron microscope (STEM)

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Email: hasan.ali-at-angstrom.uu.se Name: Hasan Ali

Organization: Uppsala University

Title-Subject: [Filtered] Scanning transmission electron microscope (STEM)

Message: Dear Colleagues,

I am a relatively new user of TEM, getting started with STEM now. As in STEM mode, the resolution
depends on probe size, so I am really curious to calculate the probe size for my STEM images. I
found a formula to calculate probe diameter using CBED discs in William's book but it requires bragg
angle (thetaB) in calculations. Is there any simple way to calculate the probe size?

Thanks

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From: microscopy.listserver-at-gmail.com
Date: Tue, 7 Jun 2016 07:54:45 -0500
Subject: [Microscopy] viaWWW:New Career Opportunities - Retained openings with leader in

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Email: sk-at-personifysearch.com Name: Sarah Kelly

Organization: Personify

Title-Subject: [Filtered] New Career Opportunities - Retained openings with leader in microscopy

Message: Hello!

We have recently had a world leading microscopy client open up several retained opportunities
throughout the US. The ideal candidate would have an expertise in microscopy/imaging, and a comfort
working directly with customers to promote the sales of the companies’ microscopy product line
(material science, forensic, and image analysis).
Please contact me directly to learn more at - sk-at-personifysearch.com or (800) 875-6188, ext.153.
Thanks!
Sarah


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From: microscopy.listserver-at-gmail.com
Date: Wed, 8 Jun 2016 18:40:03 -0500
Subject: [Microscopy] viaWWW:exploding Nalgene bottles?

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Email: dblackburn2000-at-yahoo.com Name: D Blackburn

Organization: Trinity College

Title-Subject: [Filtered] exploding Nalgene bottles?

Message: We have always stored fixed specimens of various sizes in plastic Nalgene bottles, because
they are so resistant to chemicals. However, we've recently realized that the bottles grow very
brittle with age, and can "explode" into numbers shards when squeezed slightly. I think it is a
result of age, not the stored fluids, since even bottles with 70% ethanol are brittle. Have others
experienced this? Is it a common, well-known problem?


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From: klivi-at-jhu.edu
Date: Thu, 9 Jun 2016 11:10:52 -0500
Subject: [Microscopy] EM rates and overhead

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear University Lab Managers and such,

My University is trying to decide whether it is appropriate to charge overhead on users of our facilities from both government agencies and other universities. I would like to know what the policy is for other universities, thanks,
Ken

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2, 34 -- Subject: EM rates and overhead
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From: oshel1pe-at-cmich.edu
Date: Thu, 9 Jun 2016 12:54:27 -0500
Subject: [Microscopy] EM rates and overhead

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Ken,

I think, yes, it is entirely appropriate to charge external entities overhead to for use of your instruments. I'm surprised your university is just now trying to decided if this is proper policy. In many places it is required (such as at Central Michigan, where I am). The overhead is just recovering operating costs that the university covers for its in-house people.
So it charges them to the out-house people.

Phil
________________________________________
X-from: klivi-at-jhu.edu {klivi-at-jhu.edu}
Sent: Thursday, June 09, 2016 12:33
To: Oshel, Philip Eugene

Dear University Lab Managers and such,

My University is trying to decide whether it is appropriate to charge overhead on users of our facilities from both government agencies and other universities. I would like to know what the policy is for other universities, thanks,
Ken



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From: Luc.Lajaunie-at-cnrs-imn.fr
Date: Fri, 10 Jun 2016 05:10:42 -0500
Subject: [Microscopy] New version of the EELS and XAS Database

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues,

I would like to inform you that the EELS and XAS database website has been completely rewritten and is now accessible at https://eelsdb.eu/ and can be used without registration.

We have performed many changes, in particular an improved design, a streamlined submission process and an online plotting function. An application-programming interface (API) has also been created, allowing external tools and software (such as hyperspy) to easily access the information held within the database.

I hope you will like this new version of the website and I encourage everyone to submit their spectra to the website!

Best,

Luc
--

Postdoctoral researcher
STEM-EELS specialist


Advanced Microscopy Laboratory (LMA)
Instituto de Nanocienca de Aragon (INA)
Zaragoza, España
--- The EELS database --- https://eelsdb.eu



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From: greggps-at-umich.edu
Date: Fri, 10 Jun 2016 09:54:32 -0500
Subject: [Microscopy] Fwd: viaWWW:exploding Nalgene bottles?

Contents Retrieved from Microscopy Listserver Archives
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D Blackburn,
You'll receive more accurate information from a Nalgene specialist,
but yes, plastic bottles do age out as the plasticizers come out. If
any of your bottles start to discolor or craze, I'd start to distrust
them. I believe light accelerates the degradation, and the internet
reports "denatured alchohol" as another possible accelerator. I'd also
wonder if the platicizers are affecting your samples.

They don't 'explode' as much as they simply 'shatter'. It is startling, though.

Good luck,
Gregg

Gregg Sobocinski
Microscope Imaging Specialist
University of Michigan, MCDB Dept.


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} Title-Subject: [Filtered] exploding Nalgene bottles?
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} Message: We have always stored fixed specimens of various sizes in plastic Nalgene bottles, because
} they are so resistant to chemicals. However, we've recently realized that the bottles grow very
} brittle with age, and can "explode" into numbers shards when squeezed slightly. I think it is a
} result of age, not the stored fluids, since even bottles with 70% ethanol are brittle. Have others
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From: klivi-at-jhu.edu
Date: Fri, 10 Jun 2016 15:23:59 -0500
Subject: [Microscopy] Re: EM rates and overhead

Contents Retrieved from Microscopy Listserver Archives
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Dear All,
Here is a compilation of the responses to my question (minus the out-of-office responses)…

Original message:

Dear University Lab Managers and such,

My University is trying to decide whether it is appropriate to charge overhead on users of our facilities from both government agencies and other universities. I would like to know what the policy is for other universities, thanks,
Ken
____________________________________________________
Responses (identification of places and names have been edited out)...

Here at *** we only charge overhead on industrial users, and Government users (if they should arise). Most of the time we extend the lower “in-house” rate to users from other Universities. We hope that this good will be reflected back on our users when they seek out use of other local university facilities that we may not have on campus. We do how ever not extend them a yearly maximum cap of usage fees as we would do for our internal users. If a user of our lab uses a certain number of hours per semester their billing is clipped once they’ve reached the cap. I believe this number is around 200 hours. This is easy since our microscopes are integrated into the clean room, and they may also use other systems in the lab. I hope this helps, and If you’d like more exact numbers let me know and I will search them out for you.
____________________________________________________
Our university facility has a fee structure for different user. Departmental users pay one rate, other departments and faculties another rate, and off campus users pay another higher rate. I suspect this type fee structure is similar to many other institutional facilities around the globe.

In the last round of university funding cuts (for the last 3 years) the University administration incorporated a surcharge to the fee for "Off Campus Users" of our facility. As a result, off campus users pay significantly more to use the facility and sadly the facility doesn't profit from the surcharge.
____________________________________________________
Yes to both. Every core on this campus is required to add overheads to anyone outside our institution unless there is a prior arrangement - e.g. our state *** partners.

You may want to get with the *** core. I ran the cell center there in the late 00s, and we had a schedule of charges based on where folks came from. Most were internal customers, but a couple were external, and we had to deal with the *** at ***.
Another avenue is to follow a crada-like model. This allows you to set the charges for the user specifically based on a project.
____________________________________________________
*** charges overhead on all non-commercial users from outside *** except where there is a collaborative agreement to charge internal rates at each others campuses for investigators from the other campus.
____________________________________________________
Our administration is in discussion with our immediate neighbors ( *** and *** University) about this. The 3 schools share a graduate program and so arrangements about inter-campus Cores use has always been murky.
That aside, we do charge overhead to other academic users an we double our rates to corporate users. I’ve not had to deal with government labs, so I can’t address that.
____________________________________________________
I think, yes, it is entirely appropriate to charge external entities overhead to for use of your instruments. I'm surprised your university is just now trying to decided if this is proper policy. In many places it is required (such as at ***, where I am). The overhead is just recovering operating costs that the university covers for its in-house people.
So it charges them to the out-house people.
____________________________________________________
I work in a Core and The Boss actually hired someone to come and calculate overhead. We do include overhead in work with companies or non-educational facilities; we do not charge overhead to other universities or our internal users, although the pricing structure is different. We charge a base rate for *** Internal users, a 20% discounted rate for *** Center Members, 2x rate for outside universities and (approximately) 4x rate for corporate users. Companies are expecting overhead to be built-in. Most in academics ask “What’s overhead?” ?
____________________________________________________
We have no charges for outside academic users. We do charge non-academic users.
____________________________________________________
Just in case you (or the folks calculating your rates) were not aware of this resource:

http://grants.nih.gov/grants/guide/notice-files/NOT-OD-13-053.html

Good luck. Accountants and Scientists certainly speak very different languages...
____________________________________________________
One governing rule that I thought was to guide the decision regarding this is that everyone is supposed to be charged the same rate. That means, at least for commercial entities, overhead has to be added to the hourly rate because research dollars have overhead charged on them when the grant comes into the University. Thus, it has to be added to those who don’t pay overhead up front.

However, other Universities have charged overhead on their research monies already. Following that logic, adding overhead to outside Universities would amount to charging double overhead on the research $ that a granting agency has given to any University. In effect, the granting agency and researchers would not be charged the same as others. I think this has the effect of discouraging cooperative research or forces researchers to find other ways to make rates more affordable. It also winds up hurting the core facilities since they don't often directly get the benefit of the overhead $ and use of the machines could be lessened.

However, from the viewpoint of a University’s administration, they would say that they are doing extra work without compensation. I can see validity of both sides.
____________________________________________________
We do charge hourly rates for both internal university use and outside university work, however you should look into your local regulations for these and other possible conditions:
1 We are required by state law to charge rates for outside university entities at a rate similar to a commercial analysis lab so that we are not unfairly competing.
2 Instruments acquired outside the US and imported 'duty-free' may not be used for commercial gain for a period of 5 years.
3 Instruments funded by federal grants may also have restrictions or limitations on who may be charged and what the charge may be.
____________________________________________________
At the university where I am at:

Inhouse users are charged for maintaining the instrument based on percent of usage and each user is responsible for there own preparation and supplies of materials.

Outsiders are charged extra for preparing samples (as some prep work is quick and easy while others are long and tedious) and for the time spent looking at there samples.

We conducted a study of cost and usage for In house and outside house EM work and found that universities who charge inhouse fees typically show a decrease in the amount of time the instrument is used while outside house is typically unaffected as a charge is being applied for the use of the instrument.

Many professors simply don't have the support or means to justify an EM study if there is a cheaper way to answer address the problem as EM work is often used as supporting evidence for the study that was already conducted.
____________________________________________________
We recharge off-campus users atleast 15% over the on-campus
rate, and up to the market rate. If the funding source is not on-campus,
then it is considered off-campus. Hence, having collaborators on-campus
does not impact upon the rate.

-Ken


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From: microscopy.listserver-at-gmail.com
Date: Sat, 11 Jun 2016 09:22:03 -0500
Subject: [Microscopy] viaWWW:reliability of EDS detectors

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Organization: Colorado State University

Title-Subject: [Filtered] reliability of EDS detectors

Message: Would anybody have experience or care to comment on the reliability of SDD EDS detectors v.
Si(Li) detectors? Feel free to respond off line. Thank you in advance.

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From: matthew.weyland-at-monash.edu
Date: Mon, 13 Jun 2016 00:42:18 -0500
Subject: [Microscopy] Research Fellowship available - Monash University, Australia

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Research Fellowship available - Monash University, Australia

A research fellowship is available in the development and application
of methods to characterise the atomic and electronic structure of
nanoparticles using quantitative, aberration-corrected S/TEM and
related techniques.

Full details and on-line application form can be found at:

http://www.jobs-monash.jxt.net.au/academic-jobs/research-fellow-engineering-/672529

*Deadline: Thursday 30 June 2016*

Position enquiries to:

Prof. Joanne Etheridge: joanne.etheridge-at-monash.edu
Director, Monash Centre for Electron Microscopy and
Professor, Department of Materials Science and Engineering

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From: hsia627-at-hotmail.com
Date: Tue, 14 Jun 2016 08:37:00 -0500
Subject: [Microscopy] Beginner Ultramicrotomy course at U Maryland Baltimore

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues,

We are pleased to announce that the Electron Microscopy Core Imaging Facility (EMCIF) at the University of Maryland Baltimore will be offering a Basic Ultramicrotomy mini-course on July 18th and 19th, 2016. The course is designed to teach novice users of ultramicrotome operation, trimming and sectioning of biological specimen embedded in epoxy or acrylic resin. No prior experience is required.

The course will include lectures, demonstration and (lots of) hands on practice. Registration will be limited to a maximum of six participants. More information regarding to the course and registration can be found in our website

http://www.dental.umaryland.edu/2016basicultramicrotomycourse/

An advanced cryo-ultramcrotomy course will be offered in October 17 and 18. For more information, please email coreimaging-at-umaryland.edu or visit our website http://www.dental.umaryland.edu/core-imaging/workshops-and-courses/ .

Thanks.

Best regards,

Ru-ching

Ru-ching Hsia, Ph.D.
rhsia-at-umaryland.edu
Associate Professor and Director
Core Imaging Facility
University of Maryland, Baltimore
Tel: 410-706 7992
http://www.dental.umaryland.edu/Core-imaging
Rm 696B, Howard Hall, 660 W. Redwood St. Baltimore, MD 21201

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From: lamiller-at-illinois.edu
Date: Tue, 14 Jun 2016 08:39:09 -0500
Subject: [Microscopy] UIUC's MRL Fall Bio Conference, November 2-3, Registration open to

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Greetings! Our Summer Conference went very well, and now we are geared up to start our Fall Conference.

The registration for attendees is now open as well as for exhibitors.


The “Diverse Fields of Study…. Steps Toward Medicine” themed conference will also include a student competition, as well as a whole roster of top notch speakers.


Attendees: Welcome from all educational institutions, including the student and post doc presentation competition.

$99 for first places and $50 for second places.


Information and registration can be found at:
http://mrl.illinois.edu/mrl-biological-conference-2016




Exhibitors:


_ Table in our vendor fair for 2 days, 2nd day open to any contact you wish to have come to your exhibit: $250

_ A chance to give a 30 minute presentation, Upload abstracts at registration.

__ I will see with our boss if we can broadcast slides and audio to the vendor hall during the talks.



Information and registration can be found at:
http://mrl.illinois.edu/mrl-biological-conference-2016


Please feel free to email me with any questions,

Lou Ann



{ { { { { { { { { { { { { { { { {} } } } } } } } } } } } } } } }
Lou Ann Miller
Biological Electron Microscopy
Frederick Seitz Materials Research Laboratory
Lab Room 125 Office 374 MRL
104 South Goodwin Ave
Urbana, IL 61801
Campus mail code: MC-230
Hours: 7am-3:30 pm

217-244-1567
lamiller-at-illinois.edu
http://mrl.illinois.edu


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From: microscopy.listserver-at-gmail.com
Date: Tue, 14 Jun 2016 15:42:35 -0500
Subject: [Microscopy] viaWWW:Beginner ultramicrotomy course on July 18 and 19, 2016

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X-from: rhsia-at-umaryland.edu

This Question/Comment was submitted to the Microscopy Listserver
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Email: rhsia-at-umaryland.edu Name: Ru-ching Hsia

Organization: University of Maryland, Baltimore

Title-Subject: [Filtered] Beginner ultramicrotomy course on July 18 and
19, 2016

Message: Dear Colleagues,
We are pleased to announce that the Electron Microscopy Core Imaging
Facility (EMCIF) at the University of Maryland Baltimore will be
offering a Basic Ultramicrotomy mini-course on July 18th and 19th, 2016.
The course is designed to teach novice users of ultramicrotome
operation, trimming and sectioning of biological specimen embedded in
epoxy or acrylic resin. No prior experience is required. The course
will include lectures, demonstration and (lots of) hands on practice.
Registration will be limited to a maximum of six participants. More
information regarding to the course and registration can be found in our
website
http://www.dental.umaryland.edu/2016basicultramicrotomycourse/

An advanced cryo-ultramcrotomy course will be offered in October 17 and 18.

Please email coreimaging-at-umaryland.edu for any inquiries.


Best regards,

Ru-ching

Ru-ching Hsia, Ph.D.
Associate Professor and Director
Core Imaging Facility
University of Maryland, Baltimore
Tel: 410-706 7992
http://www.dental.umaryland.edu/Core-imaging
Rm 696B, Howard Hall, 660 W. Redwood St. Baltimore, MD 21201




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From: microscopy.listserver-at-gmail.com
Date: Thu, 16 Jun 2016 00:22:04 -0500
Subject: [Microscopy] viaWWW:GSA session T6 on microanalysis and ore mineralogy

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Email: julien.allaz-at-colorado.edu Name: Julien Allaz

Organization: University of Colorado Boulder

Title-Subject: [Filtered] GSA session T6 on microanalysis and ore mineralogy

Message: Dear all,

*** apologize for cross-posting ***

We would like to direct your attention to the upcoming Annual GSA
meeting in Denver, and especially the session “T6. Micro-Analytical
Techniques in Ore Deposit Research”. Please, consider submitting an
abstract before July 12th!

http://www.geosociety.org/meetings/2016/sessions/topical.asp
This session addresses recent developments and applications of
micro­analytical techniques (e.g., SEM, EPMA, QEMSCAN, SIMS, TEM,
LA-­ICP-­MS, atom probe...) to refine our understanding of the
geochemical and temporal evolution of mineral deposits, as well as to
apply these tools to mineral exploration, mineral resource appraisal,
and mining and ore extraction. Submissions are encouraged on the
analytical techniques and methodologies themselves, as well as the
results of their use in experimental, micro­analytical, and field studies.

Invited speakers:
- Dr. Alan Koenig (USGS, Denver CO)
- Dr. Alexander Gysi (Colorado School of Mines)
- Dr. Sean Regan (USGS, Montpelier VT)

This session is co-sponsored by the Society of Economic Geologists, the
Microanalysis Society (MAS), and the GSA Mineralogy, Geochemistry,
Petrology, and Volcanology Division.

Looking forward to see you in Denver!

Katharina Pfaff (Colorado School of Mines)
Julien M. Allaz (University of Colorado Boulder)


=============================
Dr. Julien Allaz
Electron microprobe manager
University of Colorado Boulder
Dept. of Geological Sciences
UCB 399
2200 Colorado Av.
Boulder, CO 80309-0399

Phone: (303) 735 2413
Cell: (413) 210 0917
Lab: (303) 492 5251
Fax: (303) 492 2606

Personal website: http://geoloweb.ch
Lab website: http://geode.colorado.edu/~jallaz/
=============================

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From: microscopy.listserver-at-gmail.com
Date: Thu, 16 Jun 2016 00:22:50 -0500
Subject: [Microscopy] viaWWW:bar coding/sample tracking

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X-from: edward.calomeni-at-osumc.edu

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Email: edward.calomeni-at-osumc.edu Name: Ed Calomeni

Organization: Ohio State University Wexner Medical Center

Title-Subject: [Filtered] bar coding/sample tracking

Message: Hi Folks,

Our institution is in the process of implementing a bar coding/sample
tracking system. Are there any clinical EM labs out there that use such
a system? What types of steps do you track? Have you found the system
to be useful? (for what?). What systems do you have?
Any information will be greatly appreciated.
Currently we use a paper log book to keep track of samples. (Guess I am
still old school)

Thanks,

Ed
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From: microscopy.listserver-at-gmail.com
Date: Thu, 16 Jun 2016 15:03:54 -0500
Subject: [Microscopy] viaWWW:Amray 1200 C

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Email: dkuehn-at-pittstate.edu Name: David Kuehn

Organization: Pittsburg State University

Title-Subject: [Filtered] Amray 1200 C

Message: Does anyone have or know where I can get manuals for the
operation and maintenance of an Amray 1200 C SEM?


Thanks,

David Kuehn


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From: WHITTAKS-at-si.edu
Date: Fri, 17 Jun 2016 14:56:21 -0500
Subject: [Microscopy] Sputter Coater recommendations non-FESEM

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All,

Christmas in June this year-time to replace the sputter coater! As we will have this for another 20 years, and it will be in the hands of a thousand or more inexperienced users coating tens of thousands of primarily biologic samples I am soliciting feedback on potential units. Anyone enamored with a recent unit? Find it particularly easy to use or to teach others to use? Or particularly dislike some aspect of their unit? As an example- my current instrument has been a very solid performer for a loooong time- but I just can't stand the overhanging thickness monitor.

A primary consideration- if I can't get 1 page or less ikea style instructions that 90% of the researchers can understand then it is too complicated!

Anyone using the Leica E200 with experiences to share?

I have been intrigued by the osmium plasma coaters- but they seem not to have taken off outside Japan. Assuming there is a reason why? Sure seems less wasteful that the metal foil magnetron heads even though I don't run FESEM- yet.

Thanks


Scott Whittaker
Manager SEM Lab
Smithsonian Institution
National Museum of Natural History
PO Box 37012 MRC104 Room W150
Washington DC 20013-7012
202-633-0891




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From: microscopy.listserver-at-gmail.com
Date: Fri, 24 Jun 2016 07:51:32 -0500
Subject: [Microscopy] viaWWW: wrinkles/folds in LR White sections

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Title-Subject: [Filtered] wrinkles/folds in LR White sections

Message: this subject may have been discussed in this forum before, but I cannot locate anything in
the archives. The issue is sometimes when working with LR White sections, there are thin dark black
wrinkles or folds over parts of the specimen. The blanks areas of resin do not have them, only over
the specimen and mainly in organelles. I have also seen them in epoxy sections over large lipid
bodies in specimens. I'd like to know if the cause of this problem has been resolved. Thank you-
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From: jkchun-at-alliancetechgroup.com
Date: Fri, 24 Jun 2016 10:44:46 -0500
Subject: [Microscopy] SEM - Sr Microscopist Opening at Alliance Technologies (NJ)

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Alliance Technologies, a contact laboratory in NJ, has an immediate opening for a Sr. Microscopist who will characterize widely diverse samples, including organic and inorganic chemicals, household goods, pharmaceuticals, foods, plastics, etc. Analyst must execute experimental studies and deliver high quality data using a broad range of analytical methods including SEM, EDX, FTIR microscopy, Polarized Light microscopy, Fluorescence, Image analysis. Proficiency with sample preparation methods (sectioning, mounting microtome, etc.) required. Familiarity with GMP/GLP and excellent writing skills. PhD in Analytical Chemistry - 3+ years industrial experience, M.S. - 5+ years industrial experience. Background in Mineralogy a plus. Please email resume, job code, and salary history to jobs-at-alliancetechgroup.com for consideration. [16-LM-EM] Senior Scientist, Microscopy & Microanalysis at Alliance Technologies (NJ). Alliance Technologies is a DEA licensed and FDA registered contract testing laboratory in NJ which offers a wide range of chemical analysis and material testing services to a diverse, international client base. www.alliancetechgroup.com

==================
Jonathan Chun, Ph.D.
Alliance Technologies
9 Deer Park Dr. Suite B
Monmouth Jct., NJ 08852
732-355-1234
www.alliancetechgroup.com




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From: michal.jarnik-at-nih.gov
Date: Fri, 24 Jun 2016 12:40:13 -0500
Subject: [Microscopy] Anti-mCherry antibody

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Dear listers,

Could somebody suggest an anti-mCherry antibody that would work in
immunocytochemistry on,frozen sections (Tokuyasu)? Rabbit polyclonal
preferred, but not critical.

Thanks,

Michal




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From: microscopy.listserver-at-gmail.com
Date: Fri, 24 Jun 2016 18:48:05 -0500
Subject: [Microscopy] viaWWW:Hysitron picoindenter

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Email: lavoie-at-uw.edu Name: ellen lavoie

Organization: University of Washington

Title-Subject: [Filtered] Hysitron picoindenter

Message: I'm interested in getting any feedback from either current owners/users or prospective
owners/users of the Hysitron Picoindenter TEM holder. I've had an inquiry from a faculty member and
honestly I've never heard of the company. Am not necessarily exploring owning one but would like to
gather info. Please message me individually at lavoie-at-uw.edu.

Cheers,
Ellen Lavoie
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From: microscopy.listserver-at-gmail.com
Date: Fri, 24 Jun 2016 18:48:55 -0500
Subject: [Microscopy] viaWWW:Gatan PIPS 691 Argon gun upgrade

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Email: hexi-at-missouri.edu Name: Xiaoqing He

Organization: Electron microscopy core at University of Missouri

Title-Subject: [Filtered] Gatan PIPS 691 Argon gun upgrade

Message: Dear listeners,

We have an old Gatan PIPS 691 bought 24 years ago that we are trying to get back to work as normal.
It looks pretty much like the modern PIPS 691 except the knobs that are used to control the incident
angles of the guns have no readings indicating the exact angles and the gun being in the top or
bottom position. We are told that the guns are fixed and the angles relative to the sample is about
5 degrees. We are wondering if anyone in this community has some experience with upgrading the
fixed gun configurations to the adjustable ones.
Any feedback is greatly appreciated.

Xiaoqing He
Senior Research Specialist at EMC core at University of Missouri


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From: microscopy.listserver-at-gmail.com
Date: Sat, 25 Jun 2016 07:57:13 -0500
Subject: [Microscopy] viaWWW: ferromagnetic nanoparticles in TEM

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I have a user who is interested in looking at iron nanoparticles in the TEM.
I would like to know what people have done to secure the particles so that
they do not end up on my pole pieces.

I've suggested three things, but I wanted to know if there might be better
ways or which of these might be the best.

1) A long time ago, Tom Nuhfer told me that they used low mag mode and their
GIF to preserve magnetic domains in thin films, but I'm not sure that will
be high enough magnification for our researcher.

2) Use two grids of thin SiN films above and below the holey carbon films
containing the nanoparticles.

3) Embed the nanoparticles and microtome them.



-Scott

Scott D. Walck, Ph.D.
100 Matte Ln
Havre de Grace, MD 21078

S.Walck-at-comcast.net
SWalck65-at-gmail.com

(760) 685-2815 (Cell)
(443) 502-5723 (Home)



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From: r.huebner-at-hzdr.de
Date: Sat, 25 Jun 2016 07:58:59 -0500
Subject: [Microscopy] Re: viaWWW: wrinkles/folds in LR White sections

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Dear Sender,

I am not in the office until June 27th.

Best regards,
Rene Huebner.

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From: Rosemary.White-at-csiro.au
Date: Sat, 25 Jun 2016 07:58:59 -0500
Subject: [Microscopy] Re: viaWWW:EVO SEM STAGE IS NOT INITIALISED

Contents Retrieved from Microscopy Listserver Archives
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You can just go into the SmartSEM menu, open the stage tab, and initialise it manually.
cheers,
Rosemary

Dr Rosemary White
CSIRO Agriculture
GPO Box 1600
Canberra, ACT 2601
Australia
Adjunct Professor, EH Graham Centre, CSU & Research School of Biology, ANU
T 61 2 6246 5475
E rosemary.white-at-csiro.au








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From: r.huebner-at-hzdr.de
Date: Sat, 25 Jun 2016 07:58:59 -0500
Subject: [Microscopy] Re: viaWWW:bar coding/sample tracking

Contents Retrieved from Microscopy Listserver Archives
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Dear Sender,

I am not in the office until June 20th.

Best regards,
Rene Huebner.

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From: hasan.ali-at-angstrom.uu.se
Date: Sat, 25 Jun 2016 07:59:02 -0500
Subject: [Microscopy] viaWWW:Scanning transmission electron microscope

Contents Retrieved from Microscopy Listserver Archives
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Hello D. Blackburn,

I have not experienced this exact problem, but I do know that some plastic containers, such as tissue culture water bottles, do indeed deteriorate and can crack, split or break into small shards. I'm pretty sure this happens with age regardless of the fluids stored in the bottles; nor does it seem temperature-dependent. Whether or not this is a common problem, I am not sure.

I used 20ml borosilicate glass vials to store tissues. Through VWR, they are relatively inexpensive and more reliable than plastics.

Best regards,
Debra M. Townley
One Baylor Plaza, Rm. 125A
Houston Tx 77030
713-798-4679

-----Original Message-----
X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com]
Sent: Wednesday, June 08, 2016 7:07 PM
To: Townley, Debra

Dear Larry,

Thanks for your answer. I agree that the image resolution can be measured using other ways as mentioned by you, but actually I want to calculate the rpobe size in this case. I want to take some EELS spectra for a range of different probe sizes, say from 50nm-1nm. It would be very interesting for me to exactly know which probe size I have for my current image or spectra. Can I calculate the probe size from CBED discs in a simpler way?
Thanks

Regards

Hasan Ali
________________________________________
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Sent: Tuesday, June 07, 2016 5:55 PM
To: microscopy.listserver-at-gmail.com
Cc: Hasan Ali

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Email: hasan.ali-at-angstrom.uu.se Name: Hasan Ali

Organization: Uppsala University

Title-Subject: [Filtered] Scanning transmission electron microscope (STEM)

Message: Dear Colleagues,

I am a relatively new user of TEM, getting started with STEM now. As in STEM
mode, the resolution depends on probe size, so I am really curious to
calculate the probe size for my STEM images. I found a formula to calculate
probe diameter using CBED discs in William's book but it requires bragg
angle (thetaB) in calculations. Is there any simple way to calculate the
probe size?

Thanks

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From: joki-at-umich.edu
Date: Sat, 25 Jun 2016 07:59:02 -0500
Subject: [Microscopy] Re: viaWWW:exploding Nalgene bottles?

Contents Retrieved from Microscopy Listserver Archives
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Hello,

A quick look at Thermo Fisher indicates that Nalgene is not a specific
type of plastic, but a brand name.

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=14&ved=0ahUKEwiZyYP32ZnNAhULLlIKHWXOByQQFghnMA0&url=https%3A%2F%2Fwww.thermofisher.com%2Fcontent%2Fdam%2FLifeTech%2FDocuments%2FTSPromotionsfolder%2FNalgene%2520Bottles%2520and%2520Carboys%2520Technical%2520Brochure.pdf&usg=AFQjCNGmGkk7ew1C40O0xZV9Fw3jOoEong

Some bottles marketed as Nalgene are flouropolymers (FEP) and very
resistant to chemicals. Some are LDPE, which can be less so. I would
suggest checking into exactly which type of bottles you have. A
different polymer might work better. Alternatively, are the bottles
exposed to strong solvents or acids? Perhaps within the same cabinet or
fume hood? Acids in particular can do severe damage to plastic bottles.

-Jake Jokisaari


On 06/08/2016 07:02 PM, microscopy.listserver-at-gmail.com wrote:
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} Title-Subject: [Filtered] exploding Nalgene bottles?
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} Message: We have always stored fixed specimens of various sizes in plastic Nalgene bottles, because
} they are so resistant to chemicals. However, we've recently realized that the bottles grow very
} brittle with age, and can "explode" into numbers shards when squeezed slightly. I think it is a
} result of age, not the stored fluids, since even bottles with 70% ethanol are brittle. Have others
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8, 74 -- Subject: Re: [Microscopy] viaWWW:exploding Nalgene bottles?
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From: p.veys-at-cra.wallonie.be
Date: Sat, 25 Jun 2016 07:59:02 -0500
Subject: [Microscopy] viaWWW:Scanning transmission electron microscope (STEM)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I will be out of the office from 7th of June until 9th of June 2016 included.
For urgent issues regarding the EURL-AP, please contact my colleagues by email at secretary-at-eurl.craw.eu.
I will come back to you as soon as possible.


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From: MurowchickJ-at-umkc.edu
Date: Sat, 25 Jun 2016 07:59:02 -0500
Subject: [Microscopy] Re: viaWWW:exploding Nalgene bottles?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have had several low density polyethylene bottles (I dont know if they
are Nalgene brand or not) that have become brittle and will crack when
squeezed. One had had dilute nitric acid in it for a while, one had held
acetone, and others were empty on the shelf, but had been previously used.
I now check the flexibility of any LDPE bottles that I know are not new.

Jim

James B. Murowchick, Ph.D.
Associate ProfessorPrincipal Graduate Advisor and IPhD Coordinator
Department of Geosciences
University of Missouri-Kansas City
420 Flarsheim Hall
5110 Rockhill Rd
Kansas City, MO 64110 816 235-2979





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From: les-at-zsgenetics.com
Date: Sat, 25 Jun 2016 07:59:05 -0500
Subject: [Microscopy] viaWWW:Scanning transmission electron microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Ali,
Do you want to know the probe size or the image resolution? The resolution
can be measured, such as by lattice imaging or from a power spectrum. The
resolution is related to - but not identical to - the probe size. The probe
shape can be modeled in a program like QSTEM.

Regards,
Larry Scipioni

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Email: hasan.ali-at-angstrom.uu.se Name: Hasan Ali

Organization: Uppsala University

Title-Subject: [Filtered] Scanning transmission electron microscope (STEM)

Message: Dear Colleagues,

I am a relatively new user of TEM, getting started with STEM now. As in STEM
mode, the resolution depends on probe size, so I am really curious to
calculate the probe size for my STEM images. I found a formula to calculate
probe diameter using CBED discs in William's book but it requires bragg
angle (thetaB) in calculations. Is there any simple way to calculate the
probe size?

Thanks

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From: microscopy.listserver-at-gmail.com
Date: Sat, 25 Jun 2016 08:06:47 -0500
Subject: [Microscopy] Administrivia: Incorrect Email addresses for reply to:

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues

You will all notice a sudden batch of new messages today from the listserver.

I discovered all these messages had been replies to microscopy.listserver-at-gmail.com
which is a backup site and is not monitored. I'm going to have to
figure out a solution to this problem and it in park explains the quiet time
on the listserver over the last few weeks. New versions of commerical
Email programs do not always reply as had been standard in the past.

It is most likely due to something in the Email headers which the listserver embedds
and is no longer processed by Email clients as the reply to address.

Please remember if you want a reply to be on the listserver you should
send it to :

microscopy-at-microscopy.com


Sigh.....

Nestor
Your Friendly Neighborhood SysOp

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From: protrain-at-emcourses.com
Date: Sun, 26 Jun 2016 04:41:37 -0500
Subject: [Microscopy] viaWWW:Scanning transmission electron microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

Just a word of caution when trying to calculate spot size on a scanning
probe system! The resolution should not be taken as a spot size
measurement. In order to resolve a structure a number of "spots" are
required; a typical figure banded around is 4 spots.

Steve

Steve Chapman FRMS
Protrain for Consultancy and Training in Electron Microscopy
+44 (0)7711 606967
web www.emcourses.com


-----Original Message-----
X-from: hasan.ali-at-angstrom.uu.se [mailto:hasan.ali-at-angstrom.uu.se]
Sent: 25 June 2016 14:03
To: protrain-at-emcourses.com

Dear Larry,

Thanks for your answer. I agree that the image resolution can be measured
using other ways as mentioned by you, but actually I want to calculate the
rpobe size in this case. I want to take some EELS spectra for a range of
different probe sizes, say from 50nm-1nm. It would be very interesting for
me to exactly know which probe size I have for my current image or spectra.
Can I calculate the probe size from CBED discs in a simpler way?
Thanks

Regards

Hasan Ali
________________________________________
X-from: les-at-zsgenetics.com [les-at-zsgenetics.com]
Sent: Tuesday, June 07, 2016 5:55 PM
To: microscopy.listserver-at-gmail.com
Cc: Hasan Ali

X-from: hasan.ali-at-angstrom.uu.se

This Question/Comment was submitted to the Microscopy Listserver using the
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Email: hasan.ali-at-angstrom.uu.se Name: Hasan Ali

Organization: Uppsala University

Title-Subject: [Filtered] Scanning transmission electron microscope (STEM)

Message: Dear Colleagues,

I am a relatively new user of TEM, getting started with STEM now. As in STEM
mode, the resolution depends on probe size, so I am really curious to
calculate the probe size for my STEM images. I found a formula to calculate
probe diameter using CBED discs in William's book but it requires bragg
angle (thetaB) in calculations. Is there any simple way to calculate the
probe size?

Thanks

Login Host: 130.238.23.145
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From: matthew.weyland-at-monash.edu
Date: Mon, 27 Jun 2016 07:43:20 -0500
Subject: [Microscopy] RE: viaWWW:Scanning transmission electron microscope (STEM)

Contents Retrieved from Microscopy Listserver Archives
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Hi Hasan,

The 'probe size' in your instrument will depend on a number of factors.
At the nm level (not valid for aberration corrected, or even
particularly good for high end FEGTEM's) these can be summarised by the
effect of diffraction (convergence angle), spherical aberration (from
your lens) and the source limit (gun). For a simple look at all these
approximations I suggest checking out Michael and Williams, Journal of
Microscopy 147 (3), p289 (1987).

As other responders have suggested, this takes no account of your sample
and the broadening of the probe. So this is NOT the resolution. In
addition once you get into crystals and aberration corrected instruments
this is not any use.

If the concept of measuring your convergence angle via diffraction
causes you trouble (it is VERY simple, just use a standard gold
specimen..) I would suggest you approach STEM on any quantitative level
with caution!

Regards

Matthew

} X-from: hasan.ali-at-angstrom.uu.se
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} Email: hasan.ali-at-angstrom.uu.se Name: Hasan Ali
}
} Organization: Uppsala University
}
} Title-Subject: [Filtered] Scanning transmission electron microscope (STEM)
}
} Message: Dear Colleagues,
}
} I am a relatively new user of TEM, getting started with STEM now. As in STEM
} mode, the resolution depends on probe size, so I am really curious to
} calculate the probe size for my STEM images. I found a formula to calculate
} probe diameter using CBED discs in William's book but it requires bragg
} angle (thetaB) in calculations. Is there any simple way to calculate the
} probe size?
}
} Thanks

--


*Dr Matthew Weyland *
Associate Professor

*Monash Centre for Electron Microscopy and Department of Materials
Science and Engineering
*
10 Innovation Walk, Clayton Campus
Monash University
Clayton VIC 3800 Australia

T: +61 3 9905 9026
E: matthew.weyland-at-monash.edu {mailto:name.surname-at-monash.edu}
mcem.monash.edu {http://mcem.monash.edu/}

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From: rongchigram79-at-yahoo.com.sg
Date: Mon, 27 Jun 2016 10:12:16 -0500
Subject: [Microscopy] Need Advices on the Type of Anti-Static Flooring

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All, we are in process to building a low humidity (~20% RH) room to conduct cryo-tem. Due to low humidity, we are advised by others to include anti-static flooring. I would like to ask if there is any specification we need to look for anti-static flooring? Some propose one with a static electrical propensity of {2.0kV (EN 1815) and electrical resistance {10^10 ohm,may i know if is this good enough protect both human and instrument?


Best Regards
Yee Yan, Tay
FACTS, NTU
Singapore
p/s: Earlier i wrote this email in 3DEM but didn't seem to get a reply. Hope no one find it irritating for me to repost this in the microscopy channel.

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From: steven.spurgeon-at-pnnl.gov
Date: Mon, 27 Jun 2016 16:17:05 -0500
Subject: [Microscopy] Ionization Cross Sections for EDS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello everyone,

I’m looking for a table of ionization cross sections for different elements to use in some EDS calculations. Can anyone recommend a good book or paper that I might refer to?

Thanks for your help!

______________________________________
Steven R. Spurgeon, Ph.D.
Postdoctoral Research Associate
Physical and Computational Sciences Directorate

Pacific Northwest National Laboratory
902 Battelle Boulevard
P.O. Box 999 MSIN:K8-87
Richland, WA 99352

Tel: +1-509-371-7123
steven.spurgeon-at-pnnl.gov
www.stevenspurgeon.com {http://www.stevenspurgeon.com/}
www.pnnl.gov {http://www.pnnl.gov/}



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From: zaluzec-at-aaem.amc.anl.gov
Date: Mon, 27 Jun 2016 17:06:21 -0500
Subject: [Microscopy] Re: Ionization Cross Sections for EDS

Contents Retrieved from Microscopy Listserver Archives
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Steven

You did not mention the electron beam energy. However,
Cedric Powell’s most recent review paper is very good as are
most of his papers on the topic..

Use of the Bethe equation for inner-shell ionization by electron impact (2016)
Cedric J. Powell, Xavier Llovet and Francesc Salvat

American Institute of Physics (AIP)
Journal of Applied Physics
Show Details Fulltext Permalink
Publication Date: 2016-05-14

Description: We analyzed calculated cross sections for K-, L-, and M-shell ionization by electron impact to determine the energy ranges over which these cross sections are consistent with the Bethe equation for inner-shell ionization. Our analysis was performed with K-shell ionization cross sections for 26 elements, with L-shell ionization cross sections for seven elements, L3-subshell ionization cross sections for Xe, and M-shell ionization cross sections for three elements. The validity (or otherwise) of the Bethe equation could be checked with Fano plots based on a linearized form of the Bethe equation. Our Fano plots, which display theoretical cross sections and available measured cross sections, reveal two linear regions as predicted by de Heer and Inokuti [in Electron Impact Ionization, edited by T. D. Märk and G. H. Dunn, (Springer-Verlag, Vienna, 1985), Chap. 7, pp. 232–276]. For each region, we made linear fits and determined values of the two element-specific Bethe parameters. We found systematic variations of these parameters with atomic number for both the low- and the high-energy linear regions of the Fano plots. We also determined the energy ranges over which the Bethe equation can be used.

Print ISSN: 0021-8979
Electronic ISSN: 1089-7550
Topics: Physics
Published by American Institute of Physics (AIP)

Nestor
Your Friendly Neighborhood SysOp






} On Jun 27, 2016, at 4:17 PM CDT, steven.spurgeon-at-pnnl.gov wrote:
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} Hello everyone,
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} I’m looking for a table of ionization cross sections for different elements to use in some EDS calculations. Can anyone recommend a good book or paper that I might refer to?
}
} Thanks for your help!
}
} ______________________________________
} Steven R. Spurgeon, Ph.D.
} Postdoctoral Research Associate
} Physical and Computational Sciences Directorate
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} Pacific Northwest National Laboratory
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Dr. Nestor J. Zaluzec
Argonne National Laboratory
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Senior Scientist - Argonne National Laboratory
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E.P. Wigner Fellow - Oak Ridge National Laboratory
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Adjunct Professor of Physics - Northern Illinois University &
the University of Illinois at Chicago
Visiting Professor of Microscopy - Manchester University

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The box said ...
"This program requires Win 95/98/NT or better..."
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From: microscopy.listserver-at-gmail.com
Date: Mon, 27 Jun 2016 17:23:31 -0500
Subject: [Microscopy] viaWWW:Need FEI Morgagni or EM 208 specimen holders

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Email: ehaller-at-health.usf.edu Name: Edward Haller

Organization: University of South Florida

Title-Subject: [Filtered] Need FEI Morgagni or EM 208 specimen holders

Message: We had a student destroy our last good specimen holder (tip) for our FEI Morgagni TEM in
our column last week. We're using some old tips from older TEM's that we had in the lab, but they
don't work as well for sample exchange. We're looking for (hopefully a donation) of some usable FEI
Morgagni or FEI EM 208 specimen tips, if you have any sitting around and have replaced your TEM.

Edward Haller, Lab Manager
University of South Florida
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Electron Microscopy Core
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From: microscopy.listserver-at-gmail.com
Date: Mon, 27 Jun 2016 17:33:54 -0500
Subject: [Microscopy] via WWW: ferromagnetic nanoparticles in TEM

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Scott

I have had no problems containing small magnetic NP's inbetween
2 amorphous SiN windows. You can get very thin SiN now (10 nm)
from various vendors, however, if you are going to double them up
make sure you verify the thickness of the Si support frame.

I like 100 micron frames, abit fragile but fit every holders.
You can also run into 200 and 300 micron thick
frames. Those may cause problems depending upon the depth
of the "cup" in your specimen holder.

Nestor
You Friendly Neighborhood SysOp




On 6/15/16 7:27 AM CDT, Scott Walck wrote:
} I have a user who is interested in looking at iron nanoparticles in the TEM.
} I would like to know what people have done to secure the particles so that
} they do not end up on my pole pieces.
}
} I've suggested three things, but I wanted to know if there might be better
} ways or which of these might be the best.
}
} 1) A long time ago, Tom Nuhfer told me that they used low mag mode and their
} GIF to preserve magnetic domains in thin films, but I'm not sure that will
} be high enough magnification for our researcher.
}
} 2) Use two grids of thin SiN films above and below the holey carbon films
} containing the nanoparticles.
}
} 3) Embed the nanoparticles and microtome them.
}
}
}
} -Scott
}
} Scott D. Walck, Ph.D.
} 100 Matte Ln
} Havre de Grace, MD 21078
}
} S.Walck-at-comcast.net
} SWalck65-at-gmail.com
}
} (760) 685-2815 (Cell)
} (443) 502-5723 (Home)
}
}
}

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12, 51 -- Subject: via WWW: ferromagnetic nanoparticles in TEM
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From: microscopy.listserver-at-gmail.com
Date: Tue, 28 Jun 2016 19:12:47 -0500
Subject: [Microscopy] viaWWW:

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Hello Scott,

I have looked at iron nano-particles introduced into cells in culture. I processed the culture in my normal way and embedded in Spurr's Low Viscosity resin, with extra-long in filtration times. I had no trouble with the FE2++ particles staying in place during the cutting of 60nm sections and got good images on the TEM. I will say that cutting iron particles on a diamond knife will leave you with some new scratch marks on your knife :(

Debra M. Townley
One Baylor Plaza, Rm. 125A
Houston Tx 77030
713-798-4679

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Email: adam.fries-at-ucsf.edu Name: Adam Fries

Organization: UCSF

Title-Subject: [Filtered] SEM mouse skin images appear melted

Message: Hello All,

I've been working with a researcher that follows the following generic protocol to prep their mouse
skin sample:

Fixation with 2% glutaraldehyde, 4% formaldehyde in 0.1 M PO4 (ph 7.3)

1% OsO4 in water 60min
Rinse in water 5 min

ethanol 35% - 100% in steps

CPD with LCO2

and sputter coating

Problem: when imaging, some of their samples turn out great, a little less than half look melted and
'blobby'. using the same magnification, probe current, etc.

They have noticed that once out the sputter coater, all specimens appear the same. However, after
sitting for 24 hours pre-imaging, the samples that appear abnormal have turned black. I'm fairly new
to SEM, has anyone ever experienced a similar issue or can anyone offer a cause. We feel like it's
happening somewhere in the prep, just can't track it down.

Thanks!
Adam
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From: microscopy.listserver-at-gmail.com
Date: Tue, 28 Jun 2016 19:13:46 -0500
Subject: [Microscopy] viaWWW:SEM mouse skin images appear melted

Contents Retrieved from Microscopy Listserver Archives
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Email: adam.fries-at-ucsf.edu Name: Adam Fries

Organization: UCSF

Title-Subject: [Filtered] SEM mouse skin images appear melted

Message: Hello All,

I've been working with a researcher that follows the following generic protocol to prep their mouse
skin sample:

Fixation with 2% glutaraldehyde, 4% formaldehyde in 0.1 M PO4 (ph 7.3)

1% OsO4 in water 60min
Rinse in water 5 min

ethanol 35% - 100% in steps

CPD with LCO2

and sputter coating

Problem: when imaging, some of their samples turn out great, a little less than half look melted and
'blobby'. using the same magnification, probe current, etc.

They have noticed that once out the sputter coater, all specimens appear the same. However, after
sitting for 24 hours pre-imaging, the samples that appear abnormal have turned black. I'm fairly new
to SEM, has anyone ever experienced a similar issue or can anyone offer a cause. We feel like it's
happening somewhere in the prep, just can't track it down.

Thanks!
Adam
Login Host: 128.218.238.131
Listserver Email Form V - 20120416
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From: mdelann1-at-jhmi.edu
Date: Wed, 29 Jun 2016 12:55:58 -0500
Subject: [Microscopy] viaWWW:SEM mouse skin images appear melted

Contents Retrieved from Microscopy Listserver Archives
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Adam,
Do you have an image you can share? How are you mounting the specimens?
Too much adhesive (cyanoacrylate) can ride up the sides and top surface of
small tissue pieces covering them to give a "melted" appearance. The only
other thing I am leery about is unbuffered osmium (after primary fixation
buffer rinses).
Sincerely,
Michael Delannoy



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Email: adam.fries-at-ucsf.edu Name: Adam Fries

Organization: UCSF

Title-Subject: [Filtered] SEM mouse skin images appear melted

Message: Hello All,

I've been working with a researcher that follows the following generic
protocol to prep their mouse skin sample:

Fixation with 2% glutaraldehyde, 4% formaldehyde in 0.1 M PO4 (ph 7.3)

1% OsO4 in water 60min
Rinse in water 5 min

ethanol 35% - 100% in steps

CPD with LCO2

and sputter coating

Problem: when imaging, some of their samples turn out great, a little less
than half look melted and 'blobby'. using the same magnification, probe
current, etc.

They have noticed that once out the sputter coater, all specimens appear the
same. However, after sitting for 24 hours pre-imaging, the samples that
appear abnormal have turned black. I'm fairly new to SEM, has anyone ever
experienced a similar issue or can anyone offer a cause. We feel like it's
happening somewhere in the prep, just can't track it down.

Thanks!
Adam
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30, 23 -- From prvs=9812df57f=mdelann1-at-jhmi.edu Wed Jun 29 12:55:58 2016
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30, 23 -- Subject: RE: [Microscopy] viaWWW:SEM mouse skin images appear melted
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From: oshel1pe-at-cmich.edu
Date: Wed, 29 Jun 2016 13:23:19 -0500
Subject: [Microscopy] Mouse skin SEM

Contents Retrieved from Microscopy Listserver Archives
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Adam,

First, how thick and consistent are the skin preps? How much of the dermis is included?
Skin is mostly protein, keratin in particular in the outer layers, which does not take up much osmium. Dermis will contain more or less fat, which will take up osmium (especially if the fat has double-bonded carbon) and turn black. But, they'll be black coming out of the osmium. Maybe wash longer.
Also: what PO4 buffer? Na/Na2 or Na/K2? Or ... ? If you're going to use osmium, avoid K and use Sorenson's phosphate (Na/Na2), you'll have less trouble with precipitate formation. Or use Na-cacodylate buffer.

Also, try some samples without the osmium post-fix. It imparts some conductivity, but probably isn't needed for this study otherwise.

Further, the fat in the samples may be more or less extracted by the dehydration procedure. How long in each ethanol step? What steps? Don't skip the 95% step (i.e. don't go 90 =} 100%).

Variations in fat content is the most likely reason for the differences in how blackened the samples are.

But. Samples turning color in storage ... that's different. How exactly are the samples stored?
When samples turn color, do all the samples in that container turn color, or just a few?

I assume you're looking at the surface of the skin samples. Try taking some "good" ones and some "abnormal" ones (hey, right now, you don't really know which are the real ones and which are artifactual) - the dried samples, post storage, post "huh? they've turned black", and throw them in liquid nitrogen. Fracture them and VERY QUICKLY put them in a desiccator (maybe prefilled with dry N2 from a tank). You *don't* want water condensing on them. Be magical - in liquid nitrogen to in desiccator without seeing the air.
Look at the cross-section of the samples - what are the differences? This may help diagnose the problem. If nothing else, they'll be nifty to look at.

Phil

Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

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Email: adam.fries-at-ucsf.edu Name: Adam Fries

Organization: UCSF

Title-Subject: [Filtered] SEM mouse skin images appear melted

Message: Hello All,

I've been working with a researcher that follows the following generic protocol to prep their mouse
skin sample:

Fixation with 2% glutaraldehyde, 4% formaldehyde in 0.1 M PO4 (ph 7.3)

1% OsO4 in water 60min
Rinse in water 5 min

ethanol 35% - 100% in steps

CPD with LCO2

and sputter coating

Problem: when imaging, some of their samples turn out great, a little less than half look melted and
'blobby'. using the same magnification, probe current, etc.

They have noticed that once out the sputter coater, all specimens appear the same. However, after
sitting for 24 hours pre-imaging, the samples that appear abnormal have turned black. I'm fairly new
to SEM, has anyone ever experienced a similar issue or can anyone offer a cause. We feel like it's
happening somewhere in the prep, just can't track it down.

Thanks!
Adam




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From: mdelann1-at-jhmi.edu
Date: Thu, 30 Jun 2016 09:19:14 -0500
Subject: [Microscopy] Position

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Hello everyone,
We have a positon open here at the Johns Hopkins School of Medicine
Microscopy Facility for a Microscopy Facility Assistant (Requisition #:
309189).
We seek a person talented in both electron microscopy, particularly sample
preparation and ultrathin sectioning, and fluorescence confocal microscopy.
All interested candidates should apply on-line at :

https://jobs.jhu.edu/jhujobs/jobview.cfm?reqId=309189&postId=9811

Sincerely,
Michael Delannoy




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From: microscopy.listserver-at-gmail.com
Date: Thu, 30 Jun 2016 09:31:00 -0500
Subject: [Microscopy] viaWWW:Post in Transmission Electron Microscopy

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Title-Subject: [Filtered] Post in Transmission Electron Microscopy

Message: Applications are invited for the following position in the Faculty of Science +
Engineering, Department of Physics & Energy, University of Limerick, Ireland:

TITLE OF POST: Lecturer below the bar in Microscopy CONTRACT TYPE: Tenure Track (five year fixed
term). During the term of the contract the successful applicant will have the opportunity to apply
for tenure in accordance with the University's Policy and Procedures for Granting Multi-annual
Status to Entry-level Academic Staff
SALARY SCALE: €37,348 - €51,722 p.a.
QUALIFICATIONS: A doctoral degree (level 10NFQ) in physics, chemistry, materials science or a
related area.
OVERALL PURPOSE OF THE JOB: To develop new and complement existing research expertise in electron
microscopy, and undertake lecturing and administrative duties at the direction of the Head of
Department.
DESCRIPTION: The Department of Physics and Energy offers an MSc programme in Applied Physics and
undergraduate courses in Applied Physics, Mathematics & Physics, Energy, and Physics education with
either Chemistry or Biology. The Department is involved in wide-ranging research and development
programmes funded by national and European agencies. In addition to its own modern laboratories, it
has direct access to the state-of-the-art facilities of the Bernal Institute. The Bernal Institute
represents a multi-million euro investment at the University of Limerick (www.ul.ie) in Science and
Engineering (www.scieng.ul.ie), which houses world leading researchers in an appropriately equipped
experimental facility. The Department of Physics & Energy is host to the Bernal Chairs in Microscopy
and Imaging, and Energy. The successful candidate will be expected to complement and support
existing and new, emerging research directions in transmission electron microscopy and must be
experienced in the application of state-of-the-art TEM analysis to the characterisation of novel
materials for example nano-, bio-, pharma-, and medical materials. ESSENTIAL:
• A doctoral degree (level 10NFQ) in physics, chemistry, materials science or a related area.
• Have significant research experience since obtaining their PhD (or equivalent degree) and
published in first class journals. • The appointee will be capable of lecturing on first and second
year general physics and/or chemistry or materials science and in delivering advanced courses in
specialized areas of modern measurement.
• Candidates must have experience in the field of TEM electron tomography and/or holography. DESIRABLE:
• Experience in X-ray crystallography and X-ray coherent diffractive imaging would be beneficial.
The successful candidate will be expected to secure national and European funding and continue to
publish in first class journals. The appointee will undertake administrative and promotional roles
within the Department and Faculty as and when required. Applicants must demonstrate how their
research and teaching would complement existing strengths at the University of Limerick.

Further information for applicants and application material is available online from:
http://www.ul.ie/hrvacancies/
The closing date for receipt of applications via online completion is Friday, 22nd July 2016, by 12
noon, Irish Standard Time.

Please email erecruitment-at-ul.ie if you experience any difficulties; queries can also be directed to
Ursel Bangert,
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From: steven.spurgeon-at-pnnl.gov
Date: Thu, 30 Jun 2016 12:11:42 -0500
Subject: [Microscopy] Color scale in Digital Micrograph

Contents Retrieved from Microscopy Listserver Archives
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Hi everyone,

Is there an easy way to add a color scale to images in Digital Micrograph (GMS 3 in particular)? I have some results from a GPA plugin represented as a color map, but no way to produce a scale bar for that color map.

Thank you!
______________________________________
Steven R. Spurgeon, Ph.D.
Postdoctoral Research Associate
Physical and Computational Sciences Directorate

Pacific Northwest National Laboratory
902 Battelle Boulevard
P.O. Box 999 MSIN:K8-87
Richland, WA 99352

Tel: +1-509-371-7123
steven.spurgeon-at-pnnl.gov
www.stevenspurgeon.com {http://www.stevenspurgeon.com/}
www.pnnl.gov {http://www.pnnl.gov/}



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From: glenmac-at-u.washington.edu
Date: Thu, 30 Jun 2016 13:02:02 -0500
Subject: [Microscopy] specs for Nikon HFX camera controller

Contents Retrieved from Microscopy Listserver Archives
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Dear All,
I’m helping a lab convert an old Nikon microscope to DSLR. It has the tube and shutter from a Nikon UFX/HFX film camera system but the controller was apparently surplused years ago.
Does anyone know the pins and voltage for the HFX shutter so it can be opened? Then I can lock it open and a DSLR attached to the F-mount used by the old film camera.

Thanks,
Glen MacDonald
Digital Microscopy Center
Box 357923
University of Washington
Seattle, WA 98195-7923 USA
(206) 616-4156
glenmac-at-uw.edu










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From: microscopy.listserver-at-gmail.com
Date: Thu, 30 Jun 2016 17:08:44 -0500
Subject: [Microscopy] viaWWW:Long term storage of biological SEM samples

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Title-Subject: [Filtered] Long term storage of biological SEM samples

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From: stefano-at-soquelec.com
Date: Thu, 30 Jun 2016 17:58:32 -0500
Subject: [Microscopy] Color scale in Digital Micrograph

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Steven,

It is a feature that has been requested a few times and Gatan is working on it.
You can create a color scale bar with the following procedure:
1) Go to menu} file} new
2) create an image of the type "ramp Y"
3) In the image option, select the same color scheme as your image
4) Copy past the new image into your original image and use the text tool to add min, max or other values.

Regards,


Dr. Stefano Rubino,
Soquelec Limited,
Canada


-----Original Message-----
X-from: steven.spurgeon-at-pnnl.gov [mailto:steven.spurgeon-at-pnnl.gov]
Sent: June 30, 2016 10:35 AM
To: Stefano Rubino {stefano-at-soquelec.com}

Hi everyone,

Is there an easy way to add a color scale to images in Digital Micrograph (GMS 3 in particular)? I have some results from a GPA plugin represented as a color map, but no way to produce a scale bar for that color map.

Thank you!
______________________________________
Steven R. Spurgeon, Ph.D.
Postdoctoral Research Associate
Physical and Computational Sciences Directorate

Pacific Northwest National Laboratory
902 Battelle Boulevard
P.O. Box 999 MSIN:K8-87
Richland, WA 99352

Tel: +1-509-371-7123
steven.spurgeon-at-pnnl.gov
www.stevenspurgeon.com {http://www.stevenspurgeon.com/} www.pnnl.gov {http://www.pnnl.gov/}



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From: ajhall-at-prairienanotech.com
Date: Fri, 1 Jul 2016 13:55:48 -0500
Subject: [Microscopy] Re: viaWWW: Hysitron Picoindenter

Contents Retrieved from Microscopy Listserver Archives
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Dr. Lavoie,

I didn't have the opportunity to use the Hysitron Picoindenter for my
graduate work, but the University of Illinois Materials Research
Laboratory does own one that is open to all academic researchers. The
scientist in charge of that instrument is Dr. CQ Chen. I'm sure you
could contact him for more information about his experience with the
stage: cqchen-at-illinois.edu.

Wishing you continued success in your research work,
-Allen Hall
Prairie Nanotechnology

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From: microscopy.listserver-at-gmail.com
Date: Sat, 2 Jul 2016 22:54:09 -0500
Subject: [Microscopy] viaWWW:Glycocalyx Protocol for TEM

Contents Retrieved from Microscopy Listserver Archives
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Email: dyel-at-mail.nih.gov Name: Chip Dye

Organization: NIH

Title-Subject: [Filtered] Glycocalyx Protocol for TEM

Message: Hello,

Does anyone have a good protocol, for imaging using TEM, the
glycocalyx in lysosomes in mouse brain tissue (example-Purkinje cells)?
Thank you very much!
Chip Dye
Microscopy & Imaging Core
NIH/NICHD
35A Convent Drive
Bldg 35A, Rm GD931
Bethesda, MD 20892-3751
(301) 496-3627 voice


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From: nizets2-at-yahoo.com
Date: Tue, 5 Jul 2016 02:49:24 -0500
Subject: [Microscopy] viaWWW:exploding Nalgene bottles?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi!

We used plastic bottles, some were culture medium bottles, to store heavy metal containing fluids and other trash from EM for years, almost 10 years to be exact.
Some solutions were agressive, containing formaldehyde, glutaraldehyde, osmium tetroxyde or diluted acetone/ethanol. We had no Problem at all.
(the bottles were placed on trough to avoid spilling of the content in case of cracks, explosion or accident).

Regards,
Stephane


--------------------------------------------
On Sat, 6/25/16, debrat-at-bcm.edu {debrat-at-bcm.edu} wrote:

Subject: [Microscopy] RE: viaWWW:exploding Nalgene bottles?
To: nizets2-at-yahoo.com
Date: Saturday, June 25, 2016, 3:10 PM




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Hello D. Blackburn,

I have not experienced this exact problem, but I do know
that some plastic containers, such as tissue culture water
bottles, do indeed deteriorate and can crack, split or break
into small shards. I'm pretty sure this happens with age
regardless of the fluids stored in the bottles; nor does it
seem temperature-dependent. Whether or not this is a common
problem, I am not sure.

I used 20ml borosilicate glass vials to store tissues.
Through VWR, they are relatively inexpensive and more
reliable than plastics.

Best regards,
Debra M. Townley
One Baylor Plaza, Rm. 125A
Houston Tx  77030
713-798-4679

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Subject: [Microscopy] viaWWW:exploding Nalgene bottles?




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Email: dblackburn2000-at-yahoo.com
Name: D Blackburn

Organization: Trinity College

Title-Subject: [Filtered] exploding Nalgene bottles?

Message: We have always stored fixed specimens of various
sizes in plastic Nalgene bottles, because they are so
resistant to chemicals.  However, we've recently
realized that the bottles grow very brittle with age, and
can "explode" into numbers shards when squeezed
slightly.  I think it is a result of age, not the
stored fluids, since even bottles with 70% ethanol are
brittle.  Have others experienced this?  Is it a
common, well-known problem?


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From: microscopy.listserver-at-gmail.com
Date: Tue, 5 Jul 2016 06:56:11 -0500
Subject: [Microscopy] viaWWW:Tin Balls

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

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Email: dennis-at-ridesoft.com Name: Dennis Tillman

Organization: Aris Silzars Display Technology Consulting

Title-Subject: [Filtered] Tin Balls
Message: Does anyone have a "recipe" for making Tin Balls on carbon?
These are useful as test specimens. We wanted to try our hand at making
our own Tin Balls. For examples of Tin Ball test specimens I mean see
https://www.tedpella.com/calibration_html/SEM_Resolution_Test_Specimens_Tin_on_Carbon.htm
or
http://www.canemco.com/product-catalog/calibration-standards/18-products/210-sem-calibration-standards#Tin_on_Carbon_Resolution_Test_Specimen.

Thank you, Dennis Tillman (dennis-at-ridesoft.com)

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From: mdelann1-at-jhmi.edu
Date: Tue, 5 Jul 2016 08:02:47 -0500
Subject: [Microscopy] viaWWW:Glycocalyx Protocol for TEM

Contents Retrieved from Microscopy Listserver Archives
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Chip,
Look at this reference pertaining to reduced osmium and the glycocalyx:

http://www.ncbi.nlm.nih.gov/pubmed/6202662

good luck,
Michael Delannoy
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Email: dyel-at-mail.nih.gov Name: Chip Dye

Organization: NIH

Title-Subject: [Filtered] Glycocalyx Protocol for TEM

Message: Hello,

Does anyone have a good protocol, for imaging using TEM, the
glycocalyx in lysosomes in mouse brain tissue (example-Purkinje cells)?
Thank you very much!
Chip Dye
Microscopy & Imaging Core
NIH/NICHD
35A Convent Drive
Bldg 35A, Rm GD931
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From: protrain-at-emcourses.com
Date: Tue, 5 Jul 2016 10:09:53 -0500
Subject: [Microscopy] RE: viaWWW:Tin Balls

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Dennis

Have your thought of polystyrene latex, lots of sizes and as a fluid cheap
compared with tin balls? Gives you lots of options for making your own test
specimens.

Regards

Steve

Steve Chapman FRMS
Protrain for Consultancy and Training in Electron Microscopy
+44 (0)7711 606967
web www.emcourses.com

----------------------------------------------------------------------------
-----
Email: dennis-at-ridesoft.com Name: Dennis Tillman

Organization: Aris Silzars Display Technology Consulting

Title-Subject: [Filtered] Tin Balls
Message: Does anyone have a "recipe" for making Tin Balls on carbon?
These are useful as test specimens. We wanted to try our hand at making our
own Tin Balls. For examples of Tin Ball test specimens I mean see
https://www.tedpella.com/calibration_html/SEM_Resolution_Test_Specimens_Tin_
on_Carbon.htm
or
http://www.canemco.com/product-catalog/calibration-standards/18-products/210
-sem-calibration-standards#Tin_on_Carbon_Resolution_Test_Specimen.

Thank you, Dennis Tillman (dennis-at-ridesoft.com)

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17, 22 -- From protrain-at-emcourses.com Tue Jul 5 10:09:53 2016
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From: nsa2-at-leicester.ac.uk
Date: Tue, 5 Jul 2016 12:25:36 -0500
Subject: [Microscopy] EM of trichomonas protists

Contents Retrieved from Microscopy Listserver Archives
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Dear All,

A long while back (about a year) I got some very useful advise from you guys regarding the mounting of Trichomonas samples for SEM. Thank you. I found that applying glut fixed cells to a poly-L-lysine (or superfrost+) coated glass slide for 10 minutes, followed by standard SEM processing through osmium, ethanol and HMDS was fine for visualisation of the cells for SEM.

However, while the control strain (T.tenax) processed through perfectly, the test sample (unknown species of dog saliva trichomonas protists) has been a nightmare. The samples can be cultured in variety of media types, protozoan specific media and DMEM based growth medium. pH 8.5 or pH 7.2 seems to have little difference in growth rates, and although they apparently prefer a pH of 8.5, they survive adequately at a standard 7.2.

Initially, we processed the samples through 2.5%GA in DPBS PH7.2, washed 1% aqueous osmium, embedded in agar gel, then dehydrated through an ethanol series before embedding in Spurr's resin, or processed for SEM as described above. The samples weren't great - poor ultrastructure and crenelated cell membrane for the TEM, perforated membranes and collapsed appearance for the SEM, but at least they were intact.

We have since tried:

- the same process, but with a buffer pH of 8.4 (samples looked worse)
- initial fix in 0.5%GA followed by the same TEM embedding/SEM dehydration procedure but using buffered osmium (worse still)
- initial fix in Bouins fixative followed by the same procedures with buffered osmium (even worse again, as you'd expect)
- 2.5% or 5%GA in Millonigs buffer, buffered osmium at 4oC throughout (even worse)
- 2%GA/2%osmium mixture in phosphate buffer, followed by 1% osmium and usual processing all at 4oC (really really bad - not a single intact cell to be found)
- We've also tried critically point drying the cells for SEM with no discernible difference - the problem clearly lies before this step

It's quite depressing. I feel I'm probably missing something really obvious. The cells that we have observed seem to be collapsed (or sometimes inflated like a balloon), with very poorly intact membranes, and strange lamellar bodies inside the cell with no recognisable organelles (I'm assuming the lamellar bodies are what's remaining of the ultrastructural membranes). I thought it must be the glut affecting the lipid membranes, or maybe some osmotic or ion pump failure after the initial glut fix, but before the osmium stabilised the membranes ( hence the Millonigs buffer and the glut/osmium mixture in the final attempt), but every attempt seems to generate results worse than the last. Sometimes I have received the sample with no apparent cells at all, where I think that maybe the cells have disintegrated completely before reaching the EM lab. I might consider that it's the osmium causing the problems, apart from the fact that the cells have sometimes been clearly damaged before osmium exposure.

Does anyone have any suggestions of an alternative fixation protocol that may stabilise the cells? Is tannic acid our next option. Higher concentrations of sucrose?

Any suggestions are welcome!

TIA, and sorry for the long email.

Natalie

Miss Natalie Allcock
EM Facility Manager
Electron Microscopy Facility, Centre for Core Biotechnology Services,
University of Leicester, University Road, Leicester, LE1 7RH, UK


==============================Original Headers==============================
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13, 30 -- Subject: EM of trichomonas protists
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From: ZZhang-at-uwyo.edu
Date: Tue, 5 Jul 2016 14:37:01 -0500
Subject: [Microscopy] EM of trichomonas protists

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Natalie:

The collapsed appearance for SEM and the crenelated cell membrane for TEM suggest that osmolarity of the fixative might be the problem, or at least, part of the problem. The unknown species might just be more sensitive to osmotic pressure.

Here is something you can try:

Place cells in fixative, then check cells with a light microscope right away - IF you see cytoplasm "leaking" out of cells, the osmolarity is terribly wrong. GA tends to have very high osmolarity, so increase the GA concentration does not help. IF that is the case, you might want to start with paraformaldehyde. Once cells are dead and not sensitive to osmotic pressure, then switch to GA. The secondary fixative (osmium) should be less a problem.

Have you tried to use the culture medium as the "buffer" for fixative?

Hope this helps and best luck for your adventure.

Zhaojie

Zhaojie Zhang, Ph.D.
Director, Jenkins Microscopy Facility
University of Wyoming
PHONE: 307-766-3038



-----Original Message-----
X-from: nsa2-at-leicester.ac.uk [mailto:nsa2-at-leicester.ac.uk]
Sent: Tuesday, July 05, 2016 11:35 AM
To: Z.J. Zhang {ZZhang-at-uwyo.edu}

Dear All,

A long while back (about a year) I got some very useful advise from you guys regarding the mounting of Trichomonas samples for SEM. Thank you. I found that applying glut fixed cells to a poly-L-lysine (or superfrost+) coated glass slide for 10 minutes, followed by standard SEM processing through osmium, ethanol and HMDS was fine for visualisation of the cells for SEM.

However, while the control strain (T.tenax) processed through perfectly, the test sample (unknown species of dog saliva trichomonas protists) has been a nightmare. The samples can be cultured in variety of media types, protozoan specific media and DMEM based growth medium. pH 8.5 or pH 7.2 seems to have little difference in growth rates, and although they apparently prefer a pH of 8.5, they survive adequately at a standard 7.2.

Initially, we processed the samples through 2.5%GA in DPBS PH7.2, washed 1% aqueous osmium, embedded in agar gel, then dehydrated through an ethanol series before embedding in Spurr's resin, or processed for SEM as described above. The samples weren't great - poor ultrastructure and crenelated cell membrane for the TEM, perforated membranes and collapsed appearance for the SEM, but at least they were intact.

We have since tried:

- the same process, but with a buffer pH of 8.4 (samples looked worse)
- initial fix in 0.5%GA followed by the same TEM embedding/SEM dehydration procedure but using buffered osmium (worse still)
- initial fix in Bouins fixative followed by the same procedures with buffered osmium (even worse again, as you'd expect)
- 2.5% or 5%GA in Millonigs buffer, buffered osmium at 4oC throughout (even worse)
- 2%GA/2%osmium mixture in phosphate buffer, followed by 1% osmium and usual processing all at 4oC (really really bad - not a single intact cell to be found)
- We've also tried critically point drying the cells for SEM with no discernible difference - the problem clearly lies before this step

It's quite depressing. I feel I'm probably missing something really obvious. The cells that we have observed seem to be collapsed (or sometimes inflated like a balloon), with very poorly intact membranes, and strange lamellar bodies inside the cell with no recognisable organelles (I'm assuming the lamellar bodies are what's remaining of the ultrastructural membranes). I thought it must be the glut affecting the lipid membranes, or maybe some osmotic or ion pump failure after the initial glut fix, but before the osmium stabilised the membranes ( hence the Millonigs buffer and the glut/osmium mixture in the final attempt), but every attempt seems to generate results worse than the last. Sometimes I have received the sample with no apparent cells at all, where I think that maybe the cells have disintegrated completely before reaching the EM lab. I might consider that it's the osmium causing the problems, apart from the fact that the cells have sometimes been clearly damaged be!
fore osmium exposure.

Does anyone have any suggestions of an alternative fixation protocol that may stabilise the cells? Is tannic acid our next option. Higher concentrations of sucrose?

Any suggestions are welcome!

TIA, and sorry for the long email.

Natalie

Miss Natalie Allcock
EM Facility Manager
Electron Microscopy Facility, Centre for Core Biotechnology Services, University of Leicester, University Road, Leicester, LE1 7RH, UK


==============================Original Headers==============================
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From: contact-at-integrityscientific.com
Date: Tue, 5 Jul 2016 17:25:02 -0500
Subject: [Microscopy] Diffraction contrast TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear listers,
It looks like our venerable 30-year old JEOL 2000FX has come to the end of its useful life and we need to replace it.
One thing this old machine could do, which I have not been able to do in any modern microscope, is take beautiful dark field images at low magnifications ( {2000x), with perfectly parallel illumination and a large field of view.
It seems that most modern microscopes don't even go down to such low magnifications and allow dark field imaging, they just turn off the objective lens for 'low mag' mode. It is impossible to obtain low mag, diffraction contrast images on our 5-year old JEOL 2100 TEM; either the beam is too convergent, turning the diffraction condition into a stripe across the image, or the field of view is too small. (The only solution I have found to date is take lots of images and stick them together with photoshop. Time consuming for 30+ images though!)

So my question is - does anyone make a TEM that can do this any more? If so, which one? Can anyone send me a dark field image taken at 1500x on a modern TEM?

I am hoping I'm just ignorant and there's an easy answer...

Thanks

Richard



Richard Beanland
Director, Electron Microscopy
University of Warwick, UK




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From: S.Walck-at-comcast.net
Date: Tue, 5 Jul 2016 23:57:14 -0500
Subject: [Microscopy] Slow down in communication between TEM and Digital Micrograph

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers,

We have a JEOL 2100F TEM connected to a Gatan system running Digital
Micrograph (GMS 1.86). The computers are all 32 bit running Windows XP and
we are not connected to a network. We converted the system to run at 80 kV
for a few weeks and then brought it back up to 200 kV. When we did, the
power supply on our GIF was dead and it needs repair. We currently have a
loaner. I don't know when it blew and don't know whether it was related to
the voltage change. The other thing that we noticed was that the microscope
status was slow to update on DM. If we rebooted all of the computers, the
status of mag would initially change in about a second. As you use the
microscope, changing mag and modes, the update would get slower and slower
until it takes several minutes for the parameters to be updated to the
correct ones. All modes are slow, TEM, STEM, Diff, GIF, etc. This was not
happening when we were at 80 kV, only after we were back up to 200 kV.

The Gatan service engineer was not able to fix it, but he seems to remember
something like this happening before. He's checking on it. I have a
service call in to JEOL, and they seem to remember something like this
happening on another system with an Omega filter. We don't have an Omega
filter. Something is hogging the communication line between the two
systems.

Does anyone have a clue as to what might be happening?

-Scott Walck




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8, 33 -- From: "Scott Walck" {S.Walck-at-comcast.net}
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8, 33 -- Subject: Slow down in communication between TEM and Digital Micrograph
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From: oshel1pe-at-cmich.edu
Date: Wed, 6 Jul 2016 07:36:31 -0500
Subject: [Microscopy] Slow down in communication between TEM and Digital Micrograph

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Scott,

I had a similar issue once on a (non-JEOL) SEM running Gatan DM, and it turned out to be a corrupted driver in the Gatan computer. Deleting and reinstalling the driver fixed the problem.

Phil

Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

________________________________________
X-from: S.Walck-at-comcast.net {S.Walck-at-comcast.net}
Sent: Wednesday, July 6, 2016 01:17
To: Oshel, Philip Eugene

Dear Listers,

We have a JEOL 2100F TEM connected to a Gatan system running Digital
Micrograph (GMS 1.86). The computers are all 32 bit running Windows XP and
we are not connected to a network. We converted the system to run at 80 kV
for a few weeks and then brought it back up to 200 kV. When we did, the
power supply on our GIF was dead and it needs repair. We currently have a
loaner. I don't know when it blew and don't know whether it was related to
the voltage change. The other thing that we noticed was that the microscope
status was slow to update on DM. If we rebooted all of the computers, the
status of mag would initially change in about a second. As you use the
microscope, changing mag and modes, the update would get slower and slower
until it takes several minutes for the parameters to be updated to the
correct ones. All modes are slow, TEM, STEM, Diff, GIF, etc. This was not
happening when we were at 80 kV, only after we were back up to 200 kV.

The Gatan service engineer was not able to fix it, but he seems to remember
something like this happening before. He's checking on it. I have a
service call in to JEOL, and they seem to remember something like this
happening on another system with an Omega filter. We don't have an Omega
filter. Something is hogging the communication line between the two
systems.

Does anyone have a clue as to what might be happening?

-Scott Walck



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From: John.Mardinly-at-asu.edu
Date: Wed, 6 Jul 2016 16:28:24 -0500
Subject: [Microscopy] Re: Diffraction contrast TEM

Contents Retrieved from Microscopy Listserver Archives
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Richard;
It’s resolution! That’s the spec that sells, and that is what they design and build for. Pole piece designs for higher resolution tends to have smaller gaps, and then to make up for field of view loss due the smaller gaps, they have added “mini-lenses” before and after the OL. On top of that, the OL aperture is not in the back focal plane, but in a hole drilled in the lower half of the OL pole piece. These new microscopes can’t do decent dark field at any magnification, in my experience, but that is not what sells, so say adios to that capability.

John Mardinly,
Retired


} On Jul 5, 2016, at 3:42 PM, contact-at-integrityscientific.com wrote:
}
}
}
}
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} Dear listers,
} It looks like our venerable 30-year old JEOL 2000FX has come to the end of its useful life and we need to replace it.
} One thing this old machine could do, which I have not been able to do in any modern microscope, is take beautiful dark field images at low magnifications ( {2000x), with perfectly parallel illumination and a large field of view.
} It seems that most modern microscopes don't even go down to such low magnifications and allow dark field imaging, they just turn off the objective lens for 'low mag' mode. It is impossible to obtain low mag, diffraction contrast images on our 5-year old JEOL 2100 TEM; either the beam is too convergent, turning the diffraction condition into a stripe across the image, or the field of view is too small. (The only solution I have found to date is take lots of images and stick them together with photoshop. Time consuming for 30+ images though!)
}
} So my question is - does anyone make a TEM that can do this any more? If so, which one? Can anyone send me a dark field image taken at 1500x on a modern TEM?
}
} I am hoping I'm just ignorant and there's an easy answer...
}
} Thanks
}
} Richard
}
}
}
} Richard Beanland
} Director, Electron Microscopy
} University of Warwick, UK
}
}
}
}
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From: microscopy.listserver-at-gmail.com
Date: Wed, 6 Jul 2016 18:31:34 -0500
Subject: [Microscopy] viaWWW:Phosphor screen for EBSD

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Title-Subject: [Filtered] Phosphor screen for EBSD

Message: Any information on vendors who supply Phosphor screen for the EBSD camera is appreciated
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==============================End of - Headers==============================




From: colijn.1-at-osu.edu
Date: Wed, 6 Jul 2016 19:26:25 -0500
Subject: [Microscopy] viaWWW:Phosphor screen for EBSD

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



--------------------

Hendrik O. Colijn
Center for Electron Microscopy and AnalysiS
The Ohio State University
1305 Kinnear Rd, Suite 100, Columbus, OH 43212

colijn.1-at-osu.edu 614/643-3458
cemas.osu.edu {about:cemas.osu.edu}

"Time is that quality of nature which keeps things from happening all at
once." (Ray Cummings - 1922)
Lately it doesn't seem to be working.



------ Original Message ------
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From: xinran.liu-at-yale.edu
Date: Thu, 7 Jul 2016 20:46:26 -0500
Subject: [Microscopy] Director position of Cryo EM resource at Yale

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues,

Yale University is looking for a candidate to direct the new CryoEM resource. Please see below for detail.

Thank you for your attention.

Xinran Nick Liu, M.D. & Ph.D.
Director of Center for Cellular and Molecular Imaging
Bio & Cryo Electron Microscopy Core Facility
Yale University School of Medicine
Office: 203.785.4050
Lab: 203.785.5390
http://medicine.yale.edu/ccmi/em


-----------------------------------

Director, Yale University Cryo-EM Resource


Continuing a distinguished history of excellence in structural biology, Yale University now seeks a Research Scientist to direct its new University Electron Cryomicroscopy Resource. The Resource will house a Titan Krios microscope with energy filter, direct electron detectors and phase plate; the addition of a Talos Arctica is anticipated in the near future.


The Director will provide scientific leadership, management, and day-to-day direction of the Resource. The incumbent will collaborate with and support researchers from across the campus, many of whom already employ cryo-EM, by providing support and guidance in experimental design, data collection, data quality, and computational analysis as well as assisting in detailed structural analyses of biological macromolecules and complexes.


The Director will report to the offices of the Deputy Provost for Research and the YSM Deputy Dean for Academic and Scientific Affairs, with oversight by an Advisory Committee composed of senior faculty.


The Director should hold a PhD or equivalent degree in biology, chemistry, physics, biophysics, structural biology or a closely related field at the time of hire. The successful candidate should have previous experience in high-resolution structural analysis of macromolecules using cryo-EM including sample preparation and computational analysis with current and customized software; demonstrated competence in setting up, maintaining, and coordinating computational support for cryo-EM data acquisition and analysis; and experience in the optimization and use of current generation electron microscopes such as the Titan Krios.


Interested applicants should submit a CV, cover letter and three professional references to: Cryo-EM Committee, cryo.em-at-yale.edu. Review of applications will begin on August 1, 2016 and continue until the position is filled.


Yale University is an Affirmative Action/Equal Opportunity employer. Yale values diversity among its students, staff, and faculty and strongly welcomes applications from women, persons with disabilities, protected veterans, and underrepresented minorities.





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17, 68 -- From: "Liu, Xinran" {xinran.liu-at-yale.edu}
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17, 68 -- Subject: Director position of Cryo EM resource at Yale
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From: gilpin-at-purdue.edu
Date: Fri, 8 Jul 2016 10:14:28 -0500
Subject: [Microscopy] SDD EDX bellows replacement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi everyone,
I am looking for a third party solution to replace the bellows on a SDD EDX detector. Suggestions, experiences or contact from interested vendors welcome

​​​​​
Chris

Christopher J. Gilpin Ph.D.
Director, Life Science Microscopy Facility
Purdue University
Whistler Hall of Agriculture Research, Room S052
170 S. University St
West Lafayette, IN 47907
765-494-7750
gilpin-at-purdue.edu
lsmf-at-purdue.edu reaches everyone in the facility



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From: morri028-at-crimson.ua.edu
Date: Fri, 8 Jul 2016 14:49:26 -0500
Subject: [Microscopy] Fwd: Atom Probe Scientist Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

BMPC's Physical Chemistry team is seeking an experienced Atom Probe
Scientist. The selected candidate will perform atom probe research
with an emphasis on data collection, data evaluation, and overall
experimental interpretation as part of a collaborative team to
identify material characteristics, improve fundamental understanding
of material behavior, and to support development of improved
materials.

Follow link for Job description and information on how to apply:

https://bmpc.mua.hrdepartment.com/hrdepartment/ats/Posting/view/17395

All candidates must be U.S. citizens. Applicants selected will be
subject to a Federal background investigation and must meet
eligibility requirements.

Robb Morris, Ph.D.
robb.morris-at-gmail.com
Cell# 770-841-9893

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From: microscopy.listserver-at-gmail.com
Date: Sat, 9 Jul 2016 11:14:18 -0500
Subject: [Microscopy] viaWWW:Electron Microscopy Technician - Job Opening

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Email: biorlb-at-hofstra.edu. Name: Russ Burke

Organization: Hofstra University

Title-Subject: [Filtered] Electron Microscopy Technician - Job Opening

Message: School/Division: College of Liberal Arts and Sciences

Office: Biology

Full-Time or Part-Time: Part-Time

Description: The Hofstra University Department of Biology is seeks to
fill an anticipated Electron Microscopy Technician. The duties of the
Electron Microscopy Technician include but are not limited to
maintaining and using Hofstra University’s electron microscope and other
advanced scientific equipment, such as Macroscopic Solutions camera
system, GPS, particle size analyzer, elemental analyzer (XRF) and field
equipment used for teaching and research. He/she will assist faculty
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(primarily Biology, Chemistry, Geology and Bioengineering) that need
require electron micrographs. Preparation of manuals may be required
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will occasionally be required.

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times will vary as needed.

Qualifications: Experience with an ultramicrotome and a variety of other
scientific equipment, and with safe handling of chemicals is essential.

Application Instructions: Interested applicants should send their C.V.
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From: vray-at-partbeamsystech.com
Date: Sun, 10 Jul 2016 15:25:06 -0500
Subject: [Microscopy] Re: viaWWW:FIB micropillar methods

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Qiang,

Conceptually getting rid of the ditch next to the pillar edge is
straight-forward. Orient your pillar toward the SEM, and come with FIB
from the side at a glancing angle and rotate sample while shaping the
pillar.. You would not have any ditch, but instead there will be a
pyramid under the pillar. Angle of the pyramid would depend on the angle
of pillar relatively to FIB - you can even tilt the sample to a negative
angle, if you want the pyramid to be really obtuse. I could dig up a
reference on the next week - don't have access to my work computer right
now.

Practical implementation will require use of drift correction to
maintain relative position of milling box to the center of the pillar,
while sample with the pillar is rotated.


Valery Ray
www.linkedin.com/in/valeryray
==============================
PBS&T, MEO Engineering Company
290 Broadway, Suite 298
Methuen, MA 01844, USA
Phone: +1-978-305-0479 - leave a message
Mobie: +1-978-305-0479 - leave a message
E-mail: vray-at-partbeamsystech.com
Web: www.partbeamsystech.com
Web: www.freudlabs.com

On 7/10/2016 4:04 PM, Jason Wang wrote:
} Yes, I was using this way. Always having a ditch around the pillar. It's also common in some others pillars but I am thinking to remove it.
}
} On Jul 10, 2016 3:59 PM, Valery Ray {vray-at-partbeamsystech.com} wrote:
} }
} } You probably using top-down approach for shaping the pillars?
} }
} } Valery Ray
} } ==============================
} } PBS&T, MEO Engineering Company
} } 290 Broadway, Suite 298
} } Methuen, MA 01844, USA
} } Phone: +1-978-305-0479 - leave a message
} } Mobie: +1-978-305-0479 - leave a message
} } E-mail: vray-at-partbeamsystech.com
} } Web: www.partbeamsystech.com
} } Web: www.freudlabs.com
} }
} } On 7/10/2016 3:49 PM, Jason Wang wrote:
} } } Actually not yet, any suggestions?
} } }
} } } On Jul 10, 2016 3:48 PM, Valery Ray {vray-at-partbeamsystech.com} wrote:
} } }
} } } Hi Qiang,
} } }
} } } I am catching up with my mailbox - did you get explanation/reference on
} } } micropillars?
} } } --
} } } Valery Ray
} } } ==============================
} } } www.linkedin.com/in/valeryray {http://www.linkedin.com/in/valeryray}
} } } PBS&T, MEO Engineering Company
} } } 290 Broadway, Suite 298
} } } Methuen, MA 01844, USA
} } } Phone: +1-978-305-0479 - leave a message
} } } Mobie: +1-978-305-0479 - leave a message
} } } E-mail: vray-at-partbeamsystech.com
} } } Web: www.partbeamsystech.com {http://www.partbeamsystech.com}
} } } Web: www.freudlabs.com {http://www.freudlabs.com}
} } }

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From: rongchigram79-at-yahoo.com.sg
Date: Mon, 11 Jul 2016 04:32:13 -0500
Subject: [Microscopy] Slow down in communication between TEM and Digital Micrograph

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Hi Scott, we face similar problem where the magnification update becomes very slow (etc takes about 10-50s to update) but we are using DM 2.xx version. What we did is to restart the VME from our TEM side. The VME button is at the back our JEOL 2100F TEM. So just a routine is to shutdown the JEOL PC, Gatan PC, restart VME and start the JEOL PC and subsequently the Gatan PC.

Hope it helps.

Best Regards,
YY
FACTS
NTU





On Wednesday, 6 July 2016, 21:05, "oshel1pe-at-cmich.edu" {oshel1pe-at-cmich.edu} wrote:



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Scott,

I had a similar issue once on a (non-JEOL) SEM running Gatan DM, and it turned out to be a corrupted driver in the Gatan computer. Deleting and reinstalling the driver fixed the problem.

Phil

Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

________________________________________
X-from: S.Walck-at-comcast.net {S.Walck-at-comcast.net}
Sent: Wednesday, July 6, 2016 01:17
To: Oshel, Philip Eugene

Dear Listers,

We have a JEOL 2100F TEM connected to a Gatan system running Digital
Micrograph (GMS 1.86). The computers are all 32 bit running Windows XP and
we are not connected to a network. We converted the system to run at 80 kV
for a few weeks and then brought it back up to 200 kV. When we did, the
power supply on our GIF was dead and it needs repair. We currently have a
loaner. I don't know when it blew and don't know whether it was related to
the voltage change. The other thing that we noticed was that the microscope
status was slow to update on DM. If we rebooted all of the computers, the
status of mag would initially change in about a second. As you use the
microscope, changing mag and modes, the update would get slower and slower
until it takes several minutes for the parameters to be updated to the
correct ones. All modes are slow, TEM, STEM, Diff, GIF, etc. This was not
happening when we were at 80 kV, only after we were back up to 200 kV.

The Gatan service engineer was not able to fix it, but he seems to remember
something like this happening before. He's checking on it. I have a
service call in to JEOL, and they seem to remember something like this
happening on another system with an Omega filter. We don't have an Omega
filter. Something is hogging the communication line between the two
systems.

Does anyone have a clue as to what might be happening?

-Scott Walck



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13, 71 -- Subject: Re: [Microscopy] Slow down in communication between TEM and Digital
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From: klivi-at-jhu.edu
Date: Mon, 11 Jul 2016 11:48:57 -0500
Subject: [Microscopy] GIF Controller Box

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We are in need of a used Gatan 795IF CCD controller box for our GIF 200 EELS. If anyone has an old box lying around or you are replacing your old GIF with a new one, please contact me.
Thanks,
Ken

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From: steven.spurgeon-at-pnnl.gov
Date: Mon, 11 Jul 2016 13:55:05 -0500
Subject: [Microscopy] Cropping EEL spectrum in DM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi everyone,

This might be a simple question, but is there a way to crop part of an EEL spectrum in Digital Micrograph / GMS 3? It would be great if I could select a region and delete all other parts of the spectrum. Any thoughts?

Thanks!

______________________________________
Steven R. Spurgeon, Ph.D.
Postdoctoral Research Associate
Physical and Computational Sciences Directorate

Pacific Northwest National Laboratory
902 Battelle Boulevard
P.O. Box 999 MSIN:K8-87
Richland, WA 99352

Tel: +1-509-371-7123
steven.spurgeon-at-pnnl.gov
www.stevenspurgeon.com {http://www.stevenspurgeon.com/}
www.pnnl.gov {http://www.pnnl.gov/}



==============================Original Headers==============================
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8, 26 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
8, 26 -- Subject: Cropping EEL spectrum in DM
8, 26 -- Thread-Topic: Cropping EEL spectrum in DM
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From: stefano-at-soquelec.com
Date: Mon, 11 Jul 2016 16:08:28 -0500
Subject: [Microscopy] Cropping EEL spectrum in DM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

You can use a ROI to select part of your spectrum and copy/paste it back into the same window. The regions outside the ROI will be cropped (use "show legend" to select and remove the original spectrum).

Regards,


Dr. Stefano Rubino, Sales and Services
604-828-9731 /stefano-at-soquelec.com

Office:
5524 rue Saint Patrick, Suite 405, Montreal, Quebec H4E 1A8, Canada
514-482-6427ext 230/ Fax:514-482-1929/https://www.soquelec.com
https://en.wikipedia.org/wiki/Soquelec / https://stefanorubinophd.wordpress.com/





-----Original Message-----
X-from: steven.spurgeon-at-pnnl.gov [mailto:steven.spurgeon-at-pnnl.gov]
Sent: July 11, 2016 12:20 PM
To: Stefano Rubino {stefano-at-soquelec.com}

Hi everyone,

This might be a simple question, but is there a way to crop part of an EEL spectrum in Digital Micrograph / GMS 3? It would be great if I could select a region and delete all other parts of the spectrum. Any thoughts?

Thanks!

______________________________________
Steven R. Spurgeon, Ph.D.
Postdoctoral Research Associate
Physical and Computational Sciences Directorate

Pacific Northwest National Laboratory
902 Battelle Boulevard
P.O. Box 999 MSIN:K8-87
Richland, WA 99352

Tel: +1-509-371-7123
steven.spurgeon-at-pnnl.gov
www.stevenspurgeon.com {http://www.stevenspurgeon.com/} www.pnnl.gov {http://www.pnnl.gov/}



==============================Original Headers==============================
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From: microscopy.listserver-at-gmail.com
Date: Mon, 11 Jul 2016 17:17:40 -0500
Subject: [Microscopy] viaWWW:Position open for Materials Characterization Technician

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: lthompson-at-us.ibm.com

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Email: lthompson-at-us.ibm.com Name: Leslie Thompson

Organization: IBM Research-Almaden

Title-Subject: [Filtered] Position open for Materials Characterization Technician

Message: We have an opening for a FIB/SEM technician, including EDX and 3D reconstruction, at IBM
Research-Almaden in San Jose, CA. Applications accepted only through the website.

https://krb-sjobs.brassring.com/TGnewUI/Search/home/HomeWithPreLoad?PageType=JobDetails&partnerid=26059&siteid=5016&jobid=61234&AReq=52775BR&Codes=SmartRecruiters
Materials Characterization Technician
52775BR
Job Description
The Materials characterization team at IBM Almaden Research Center in San Jose, CA has an
immediate opening for an experienced SEM/FIB engineer or technician. The work will include SEM
imaging, EDX analysis, FIB/TEM preparation, and 3D reconstruction of images using serial acquisition
techniques on an FEI Helios 400S Nanolab Dualbeam system. The position is a long-term supplemental
(LTS) , and partially off-shift (includes evening hours) with some schedule flexibility as to full
or part time. Mechanical sample preparation skills for SEM or TEM is desirable, as well as
additional characterization experience (Auger, XPS, SIMS, AFM). US Citizenship is required for this
position.

This position requires the analyst to work independently, developing creative characterization
solutions to a wide variety of sample types. Successful candidates will perform meticulous data
generation, communicate the findings effectively to experts and non-experts, and work well with
other team members to answer challenging analysis problems.

The World is Our Laboratory No matter where discovery takes place, IBM researchers push the
boundaries of science, technology and business to make the world work better. IBM Research is a
global community of forward-thinkers working towards a common goal: progress.


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From: hsia627-at-hotmail.com
Date: Tue, 12 Jul 2016 07:53:06 -0500
Subject: [Microscopy] EM Facility research assistant position open

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues,

I would like to draw your attention that a research assistant position at the Electron Microscopy Core Imaging Facility (EMCIF) of University of Maryland Baltimore is currently open for application. If you are interested, please go to the link below for the job description and apply online.

https://umb.taleo.net/careersection/umb_external_staff/jobdetail.ftl?job=160000OM&lang=en

Thanks.

Best regards,

Ru-ching

Ru-ching Hsia, Ph.D.
rhsia-at-umaryland.edu
Associate Professor and Director
Electron Microscopy Core Imaging Facility
University of Maryland, Baltimore
Tel: 410-706 7992
http://www.dental.umaryland.edu/Core-imaging
Rm 696B, Howard Hall, 660 W. Redwood St. Baltimore, MD 21201

==============================Original Headers==============================
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7, 19 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
7, 19 -- Subject: EM Facility research assistant position open
7, 19 -- Date: Tue, 12 Jul 2016 08:55:59 -0400
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From: jhayden-at-Wistar.org
Date: Tue, 12 Jul 2016 11:02:58 -0500
Subject: [Microscopy] Research Assistant Position available at The Wistar Institute

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues,

The Wistar Institute in Philadelphia currently has an opening for a Research Assistant in the Imaging Core Facility:
The Imaging Shared Resource at The Wistar Institute is a growing core facility in a dynamic research institution that provides access to standard and advanced optical imaging systems, custom image analysis solutions, individualized training and technical support for a wide range of advanced imaging equipment. The Facility offers a complete soup to nuts approach, offering recommendations on experimental design, best practices for image acquisition, image analysis and data processing, as well as final presentation options. We are currently searching for a dedicated and enthusiastic Research Assistant to join our imaging team to help support cutting-edge cancer research and vaccine development and get the most out of modern imaging methods.

The successful candidate will be primarily responsible for training and assistance with acquisition and software analysis using our PerkinElmer IVIS small animal imaging system as well as assistance with a wide range of microscopy and photographic systems.

We are looking for a bachelors or masters level applicant in biological or physical sciences with at least 1 year of experience in a research environment, a passion for learning, helping, and running cutting-edge technology. Experience with fluorescence and luminescence systems is highly desirable, with a multidisciplinary skill set beneficial (cell biology, image analysis, biomedical engineering, biochemistry, life sciences, biophysics and programming). Confidence and experience assisting with small animals (primarily mice) in a barrier facility environment, experience with imaging systems and image analysis, strong computing skills are preferred, experience in fluorescence imaging techniques, and in confocal microscopy are a plus.

Major roles include, but are not limited to: advising, training and assisting researchers in small animal imaging techniques, assistance with widefield and confocal microscopy systems, brightfield and fluorescence microscopy, traditional macro and photographic documentation, assistance with quantitative image analysis, collaboration with researchers to troubleshoot and optimize experimental design, maintaining/calibrating/optimizing imaging equipment, tracking equipment usage data.

The Wistar Institute is located in the University City area of Philadelphia, in the heart of the University of Pennsylvania Campus. Wistar provides resources to its faculty and staff that enable them to conduct cutting edge collaborative research and provides for outstanding intellectual environments and state-of-the-art facilities. Research discoveries conducted at Wistar have led to the development of vaccines; the identification of genes associated with cancers; and the development of many other significant research technologies and tools.

For more information and to apply, please visit our website at www.wistar.org and check in the employment link.

*********************************
James Hayden, RBP, FBCA
Managing Director, Imaging Shared Resource
The Wistar Institute
3601 Spruce St.
Philadelphia, PA 19104
(215)898-3887
jhayden-at-wistar.org










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15, 54 -- From: James Hayden {jhayden-at-Wistar.org}
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15, 54 -- Subject: Research Assistant Position available at The Wistar Institute
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From: Reinhard.Rachel-at-biologie.uni-regensburg.de
Date: Tue, 12 Jul 2016 12:15:13 -0500
Subject: [Microscopy] Slow down in communication between TEM and

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Colleagues,

this is just to add a comment about our microscope ... also a JEOL JEM2100F (2012), running the JEOL TEMCON software for controlling, with TVIPS camera and Software, and also SerialEM installed. No Gatan Software / hardware involved (except the Gatan 626 cryo-transfer holder).

Occasionally, - once in a month or two - we face similar problems that on the JEOL PC in TEMCON, execution of "loading the alignment file" can be very very slow (} } 30 sec or much longer), instead of 2 to 5 sec, as it should be.
What we do is to restart the PC (not the VME) controlling the JEOL - usually, this is sufficient.
Rarely, we also have to re-boot the VME of the JEOL.
Then, the routine is to shutdown the TVIPS PC, then the JEOL PC, restart VME and start the JEOL PC and subsequently the TVIPS PC.
kind regards,
Reinhard

--
Prof. Dr. Reinhard Rachel
University of Regensburg
Centre for EM / Anatomy
Faculty of Biology & Preclin. Med.
Universitaetsstrasse 31
D-93053 Regensburg - Germany
tel +49 941 943 -2837, -1720
mail reinhard.rachel-at-biologie.uni-regensburg.de
office: VKL 3.1.29

Next microscopy conferences:
- 16th Europ Microsc Congress EMC
http://www.emc2016.fr/en/
28 Aug - 2 Sept 2016 in Lyon, FR
- Microscopy Conference 2017
Dreilaendertagung Lausanne, CH
20-25 August 2017
- next Microbiol. conferences:
VAAM/DGHM - Annual Conf
March 5-8, 2017, Wuerzburg




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From: microscopy.listserver-at-gmail.com
Date: Tue, 12 Jul 2016 21:53:08 -0500
Subject: [Microscopy] viaWWW:FIB Technician Opening

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Email: ming_j_zhang-at-amat.com Name: Ming Zhang

Organization: Applied Materials Company

Title-Subject: [Filtered] FIB Technician Opening

Message: Applied Materials Company has an immediate opening for an FIB technician in Portland, Oregon.

Applied Materials Company is the world's leading semiconductor equipment manufacturing company and
one of top 500 U.S. Companies.

The successful candidate will cover weekend and early night shifts and mainly focus on TEM sample
preparations.
A minimum of associate degree in Engineering, Materials Science, Chemistry, or Physics, etc. is
required.
US citizen or green card holder preferred.

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From: greggps-at-umich.edu
Date: Wed, 13 Jul 2016 07:50:44 -0500
Subject: [Microscopy] Re: Slow down in communication between TEM and

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Reinhard,
Is your computer networked? Is it a Windows system? I experience
interruptions with my camera and other hardware drivers when certain
automatic updates try to load on my confocal and other microscope
computers. For a few years, I had regular "camera not found" messages
on one of my research microscopes, and our computer people believe it
was certain updates, which disconnect hardware while it installs. I
blame Microsoft the most, but never tracked which vendor updates
caused the problem. The problem occurs when the update is downloaded,
but installation is not complete.

Look for the yellow triangle at the lower right corner of your monitor
on the start bar, and reboot whenever you see it. I've had scanning
confocal imaging systems stop in the middle of a scan because the
update just happened to come at that time.

Your problem may be completely different, since it's an alignment file
issue. My problems mostly went away when those equipment computers
were taken off the network.

Good luck,
~Gregg

Gregg Sobocinski
Microscope Imaging Specialist
University of Michigan, MCDB Dept.
Ann Arbor, Michigan, USA


On Tue, Jul 12, 2016 at 1:28 PM,
{Reinhard.Rachel-at-biologie.uni-regensburg.de} wrote:
}
}
}
}
} ----------------------------------------------------------------------------
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}
} Hi Colleagues,
}
} this is just to add a comment about our microscope ... also a JEOL JEM2100F (2012), running the JEOL TEMCON software for controlling, with TVIPS camera and Software, and also SerialEM installed. No Gatan Software / hardware involved (except the Gatan 626 cryo-transfer holder).
}
} Occasionally, - once in a month or two - we face similar problems that on the JEOL PC in TEMCON, execution of "loading the alignment file" can be very very slow (} } 30 sec or much longer), instead of 2 to 5 sec, as it should be.
} What we do is to restart the PC (not the VME) controlling the JEOL - usually, this is sufficient.
} Rarely, we also have to re-boot the VME of the JEOL.
} Then, the routine is to shutdown the TVIPS PC, then the JEOL PC, restart VME and start the JEOL PC and subsequently the TVIPS PC.
} kind regards,
} Reinhard
}
} --
} Prof. Dr. Reinhard Rachel
} University of Regensburg
} Centre for EM / Anatomy
} Faculty of Biology & Preclin. Med.
} Universitaetsstrasse 31
} D-93053 Regensburg - Germany
} tel +49 941 943 -2837, -1720
} mail reinhard.rachel-at-biologie.uni-regensburg.de
} office: VKL 3.1.29
}
} Next microscopy conferences:
} - 16th Europ Microsc Congress EMC
} http://www.emc2016.fr/en/
} 28 Aug - 2 Sept 2016 in Lyon, FR
} - Microscopy Conference 2017
} Dreilaendertagung Lausanne, CH
} 20-25 August 2017
} - next Microbiol. conferences:
} VAAM/DGHM - Annual Conf
} March 5-8, 2017, Wuerzburg
}
}
}
}
} ==============================Original Headers==============================
} 7, 25 -- From Reinhard.Rachel-at-biologie.uni-regensburg.de Tue Jul 12 12:15:13 2016
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} 7, 25 -- Date: Tue, 12 Jul 2016 19:17:59 +0200
} 7, 25 -- From: "Reinhard Rachel" {Reinhard.Rachel-at-biologie.uni-regensburg.de}
} 7, 25 -- To: "microscopy server" {Microscopy-at-microscopy.com}
} 7, 25 -- Subject: Slow down in communication between TEM and
} 7, 25 -- References: {201607110932.u6B9WpO9030382-at-ns.microscopy.com}
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From: microscopy.listserver-at-gmail.com
Date: Fri, 15 Jul 2016 08:09:18 -0500
Subject: [Microscopy] viaWWW: Is two-photon microscopy maintenance costs high?

Contents Retrieved from Microscopy Listserver Archives
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Email: stlervin-at-yeah.net Name: Ervin

Title-Subject: [Filtered] Is two-photon microscopy maintenance costs high?

Message: Hi!
Is anybody knows the mantennace costs of the two-photon microscopy? How about it compared with point
scanning confocal? And last one, how long is the lifetime of the laser line generator.
Thanks!

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From: colijn.1-at-osu.edu
Date: Fri, 15 Jul 2016 14:27:15 -0500
Subject: [Microscopy] M&M Airport transportation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,

FYI...

COTA recently instituted a dedicated bus route to/from the airport which
goes to the Convention Center and some hotels. Cost is $2.75. You can
find details at: http://www.cota.com/Riding-COTA/AirConnect.aspx

Note that they also have a free bus loop that goes along High Street
past the Convention Center. It's an easy way to get to nearby
restaurants, etc. Again details are at:
http://www.cota.com/Riding-COTA/CBUS.aspx

Cheers,
Henk



--------------------

Hendrik O. Colijn
Center for Electron Microscopy and AnalysiS
The Ohio State University
1305 Kinnear Rd, Suite 100, Columbus, OH 43212

colijn.1-at-osu.edu 614/643-3458
cemas.osu.edu

"Time is that quality of nature which keeps things from happening all at
once." (Ray Cummings - 1922)
Lately it doesn't seem to be working.



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From: microscopy.listserver-at-gmail.com
Date: Sun, 17 Jul 2016 14:36:55 -0500
Subject: [Microscopy] viaWWW: Scientist II position available

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Email: danb-at-alleninstitute.org Name: Dan Bumbarger

Organization: Allen Institute for Brain Science

Title-Subject: [Filtered] Scientist II position available

Message: Our group has a scientist II position available immediately. We are scaling up high
throughput transmission electron microscopy techniques to produce petabyte scale datasets of
vertebrate cortex synaptic wiring. The advertised position could fill several different roles in our
team. We would strongly prefer someone that would be passionate about helping to maximize uptime on
our highly customized JEOL microscopes. We strongly prefer experience diagnosing problems and a lack
of fear of taking a wrench to the microscopes. Also highly desireable is someone with skills in
dealing with the computational and software side of our pipeline. Another type of role that could be
filled would be to manage contractor work geared towards modifying the electron optics and other
aspects of the system. We are gearing up to do some very big things, it would be a great time to
join our team! Please check the Allen Institute website to apply, or contact me directly.
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From: oshel1pe-at-cmich.edu
Date: Wed, 20 Jul 2016 12:09:18 -0500
Subject: [Microscopy] Anyone need film carriers and cartridges for Philips TEMs?

Contents Retrieved from Microscopy Listserver Archives
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I will be driving to M&M this weekend, and if anyone needs or has a use for negative holders and cartridges for Philips 300/400 and CM series TEMs, I have lots of them. Free to good homes.
But ... I have to know by Friday afternoon (the 22nd).

Also, if anyone is still developing negatives, I have a pile of negative hangers.

Phil

Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576


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From: microscopy.listserver-at-gmail.com
Date: Thu, 21 Jul 2016 16:41:14 -0500
Subject: [Microscopy] viaWWW:SAP pole piece JEOL 1200

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Email: danb-at-alleninstitute.org Name: Dan Bumbarger

Organization: Allen Institute for Brain Science

Title-Subject: [Filtered] SAP pole piece JEOL 1200

Message: Does anyone have a SAP pole piece that fits a JEOL 1200 TEM?
We'd like to do some experiments where we widen the apertures for
specimen insertion to further our development of high capacity/high
throughput specimen holders. Difficult to find one without purchasing an
entire column..
Thanks in advance,

-Dan

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From: microscopy.listserver-at-gmail.com
Date: Thu, 21 Jul 2016 16:42:13 -0500
Subject: [Microscopy] viaWWW:SEM need help to identify weird things on pollen surface

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Email: xuanhao.sun-at-uconn.edu Name: Xuanhao Sun

Organization: University of Connecticut

Title-Subject: [Filtered] SEM need help to identify weird things on
pollen surface

Message: Hi all,

We need some help from all you veteran microscopists on this listserver
to identify some weird structures we saw on the pollen surface, which
kinda like twist wrapping candies… The pollen grains were dissected out
from a closed compartment of the plant before mounting, so these
structure should come from inside the plant along with the pollen.
Please see the links below. Any suggestion will be appreciated!

http://emlab.uconn.edu/wp-content/uploads/sites/370/2016/07/Ryssopterys-15_52.jpg

http://emlab.uconn.edu/wp-content/uploads/sites/370/2016/07/Ryssopterys-15_53.jpg

http://emlab.uconn.edu/wp-content/uploads/sites/370/2016/07/Ryssopterys-15_54.jpg


Xuanhao Sun, Ph.D.
Bioscience Electron Microscopy Laboratory
University of Connecticut
BPB G06, Unit 3242
Storrs, CT 06269-3242
Office Tel: 860-486-6368
Lab Tel: 860-486-2914
Fax: 860-486-6369
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From: Rosemary.White-at-csiro.au
Date: Thu, 21 Jul 2016 18:11:03 -0500
Subject: [Microscopy] Re: viaWWW:SEM need help to identify weird things on

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Xuanhao,

In morphology, they look like tiny pollen, which does come in that sort of shape. Forget-me-not pollen is this small. But then the flower was unopened. A mystery.

cheers,
Rosemary

Dr Rosemary White
CSIRO Black Mountain
GPO Box 1600
Canberra, ACT 2601
Australia

T 61 2 6246 5475
E rosemary.white-at-csiro.au






On 22/07/2016 7:56 am, "microscopy.listserver-at-gmail.com" {microscopy.listserver-at-gmail.com} wrote:

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13, 71 -- Subject: Re: [Microscopy] viaWWW:SEM need help to identify weird things on
13, 71 -- pollen surface
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From: ph2-at-sprynet.com
Date: Thu, 21 Jul 2016 18:28:17 -0500
Subject: [Microscopy] viaWWW:SEM need help to identify weird things on

Contents Retrieved from Microscopy Listserver Archives
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Looks like spores of the fungus Pithomyces.



..
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Sent: Thursday, July 21, 2016 5:55 PM
To: ph2-at-sprynet.com

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Email: xuanhao.sun-at-uconn.edu Name: Xuanhao Sun

Organization: University of Connecticut

Title-Subject: [Filtered] SEM need help to identify weird things on pollen
surface

Message: Hi all,

We need some help from all you veteran microscopists on this listserver to
identify some weird structures we saw on the pollen surface, which kinda
like twist wrapping candies… The pollen grains were dissected out from a
closed compartment of the plant before mounting, so these structure should
come from inside the plant along with the pollen.
Please see the links below. Any suggestion will be appreciated!

http://emlab.uconn.edu/wp-content/uploads/sites/370/2016/07/Ryssopterys-15_5
2.jpg

http://emlab.uconn.edu/wp-content/uploads/sites/370/2016/07/Ryssopterys-15_5
3.jpg

http://emlab.uconn.edu/wp-content/uploads/sites/370/2016/07/Ryssopterys-15_5
4.jpg


Xuanhao Sun, Ph.D.
Bioscience Electron Microscopy Laboratory University of Connecticut BPB
G06, Unit 3242 Storrs, CT 06269-3242
Office Tel: 860-486-6368
Lab Tel: 860-486-2914
Fax: 860-486-6369
website: emlab.uconn.edu

Login Host: 137.99.47.82
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From: eikonika-at-otenet.gr
Date: Fri, 22 Jul 2016 03:04:05 -0500
Subject: [Microscopy] RE: viaWWW:SEM need help to identify weird things on pollen surface

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Hi Xuanhao
Did you mount the pollen straight from the plant or you did before some
preparation for SEM?
yorgos


Dr Yorgos Nikas
Athens Innovative Microscopy
Skra 36 Voula 16673 GREECE

Tel/fax +30 210 8957677
mobile +30 6945 107477
www.eikonika.net www.aim.cat
*************************************






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From: henning.stahlberg-at-unibas.ch
Date: Mon, 25 Jul 2016 03:20:29 -0500
Subject: [Microscopy] Postdoc / Engineer's position in high-end cryo-EM data collection

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We have an opening for a PostDoc / Engineer to support the high-resolution data collection on a Titan Krios and a Polara, using manual and automated operation. Various membrane protein systems are to be imaged in single particle and electron tomography approaches.

Our lab is part of the Biozentrum of the University of Basel in Switzerland, at the border towards Germany and France. The Biozentrum of the University of Basel is one of the leading institutes worldwide for molecular and biomedical basic research and teaching. It is home to more than 30 research groups with scientists from over 40 countries. Research at the Biozentrum focuses on the areas of Cell Growth & Development, Infection Biology, Neurobiology, Structural Biology & Biophysics and Computational & Systems Biology. With its more than 550 employees, the Biozentrum is the largest department at the University of Basel's Faculty of Science.

The Stahlberg group at the Center for Cellular Imaging and NanoAnalytics (C-CINA) of the Biozentrum studies Parkinson's disease and the structure of membrane protein systems by cryo-transmission electron microscopy (EM). Our methods include high-resolution cryo-EM imaging and image processing, to determine the structure of neuronal tissue by electron tomography, or to determine the atomic structure of the investigated ion channels and transporter membrane protein systems. For this purpose, we operate a FEI Titan Krios, equipped with a Gatan GIF-LS and K2-Summit detector, and also a FEI Polara, equipped with another K2 Summit.

To support operation of these two instruments, and to perform manual datacollection and to setup and run automated datacollection, we are looking to recruit a highly motivated PostDoc or Engineer.
Your responsibilities are
- Vitrification of cryo-EM samples (FEI Vitrobot, Leica GP).
- Operation of the Titan Krios in alignment and manual data collection.
- Setup and run SerialEM to collect electron tomography dataset and single particle cryo-EM datasets in an automated manner.
- Training of students in the above methods.
- Optionally: Data processing: Drift correction, particle picking, 3D reconstruction. These steps are partly implemented in an automated pipeline, based on Zorro (Robb McLeod et al.).

Your profile
Applicants should have experience in cryo-EM data collection. Knowledge of computer automation of workflows (e.g., SerialEM) is also required. The primary language in the laboratory is English.

The positions are funded for 2 years, an extension is possible. For further details, please contact me offline.

Henning Stahlberg
Center for Cellular Imaging and NanoAnalytics (C-CINA)
Biozentrum, University of Basel
Switzerland
Henning.Stahlberg-at-unibas.ch
http://c-cina.org




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From: microscopy.listserver-at-gmail.com
Date: Mon, 1 Aug 2016 18:47:23 -0500
Subject: [Microscopy] viaWWW: Advice Needed Transmission electron microscope cm-10

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Email: researchers4u-at-gmail.com
Name: Sanaz shobeikeh

Organization: Shiraz university, iran

Title-Subject: [Filtered] Transmission electron microscope cm-10 alignment, beam disappear in M
magnification range with object aperture in
Message: Hi:
Sorry to bother you i just have replaced the cm-10 tungsten filament and now i'my doing its
alignment, but now I have found out that the student that was working with the microscope when it's
filament got burnt got panicked and pressed the hard reset small push button with its pen tip. For
now i found the beam with gun shift on filament heating of 19 and 20,i have done the gun procedure
and got the beam even after i entered C2 aperture. but in higher mags (M range and higher) with obj.
aperture in the beam way, i am facing two obstacles :first i can't move the beam with shift x/y and
INT volume , and second when i try to move the beam to the center of big screen with mechanical obj.
x/y controls, it's like something is in its way (something round) something dark that covers center
of phosphorus big screen. i was thinking to correct the C2 centration so i become sure this has
nothing to do with obj. aperture problem. for now i have no beam intensity in high magnifications (M
range and higher) but in low mag range there is a round and green beam. I would appreciate any
advice. Best regards
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From: CReplogle-at-asbestostemlabs.com
Date: Tue, 2 Aug 2016 13:31:26 -0500
Subject: [Microscopy] viaWWW: Advice Needed Transmission electron microscope cm-10

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With our CM-12 filaments, I sometimes have to take out and readjust new filaments after they have initially been installed. The filament alignment can change too much with initial heating to adjust it with just the shift x,y knobs. Also, I would try manually adjusting the filament a little further back from the aperture, and then using the emission settings to bring it closer as you do alignment. Sometimes wI find our filaments are set a little too close to the aperture.


Crystal Replogle
TEM Technical Manager
Asbestos TEM Laboratories

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Email: researchers4u-at-gmail.com
Name: Sanaz shobeikeh

Organization: Shiraz university, iran

Title-Subject: [Filtered] Transmission electron microscope cm-10 alignment, beam disappear in M magnification range with object aperture in
Message: Hi:
Sorry to bother you i just have replaced the cm-10 tungsten filament and now i'my doing its alignment, but now I have found out that the student that was working with the microscope when it's filament got burnt got panicked and pressed the hard reset small push button with its pen tip. For now i found the beam with gun shift on filament heating of 19 and 20,i have done the gun procedure and got the beam even after i entered C2 aperture. but in higher mags (M range and higher) with obj.
aperture in the beam way, i am facing two obstacles :first i can't move the beam with shift x/y and INT volume , and second when i try to move the beam to the center of big screen with mechanical obj.
x/y controls, it's like something is in its way (something round) something dark that covers center of phosphorus big screen. i was thinking to correct the C2 centration so i become sure this has nothing to do with obj. aperture problem. for now i have no beam intensity in high magnifications (M range and higher) but in low mag range there is a round and green beam. I would appreciate any advice. Best regards
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10, 53 -- Subject: viaWWW: Advice Needed Transmission electron microscope cm-10 10, 53 -- alignment, beam disappear in M magnification range with object aperture in 10, 53 -- References: {201608011529.u71FTD7o025749-at-ns.microscopy.com}
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19, 33 -- From CReplogle-at-asbestostemlabs.com Tue Aug 2 13:31:23 2016
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19, 33 -- Subject: RE: [Microscopy] viaWWW: Advice Needed Transmission electron
19, 33 -- microscope cm-10
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19, 33 -- microscope cm-10
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From: cni-at-udel.edu
Date: Tue, 2 Aug 2016 16:39:45 -0500
Subject: [Microscopy] viaWWW: Advice Needed Transmission electron microscope cm-10

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Also, if you've exhausted all the tricks, the following should definitely work provided there are no failed parts:

1. All the apertures out (they should surely be out);
2. Bottom up, turn off major lenses one by one till you see the beam or a weak flickering spot (needle size);
3. Top down, turn on the lenses one by one, and maximize the brightness at each step.

*******************************************************
Chaoying Ni, PhD
Director, W. M. Keck Center for Advanced Microscopy and Microanalysis
Professor, Department of Materials Science and Engineering
http://www.camm.udel.edu; http://www.mseg.udel.edu
(302) 831-6359(Phone); (302) 831-4545 (Fax)

Facility location:
154-174 Harker Laboratory
221 Academic Street, Newark, DE 19716

Mailing address:
University of Delaware
201 duPont Hall, Newark, DE 19716
*******************************************************

-----Original Message-----
X-from: CReplogle-at-asbestostemlabs.com [mailto:CReplogle-at-asbestostemlabs.com]
Sent: Tuesday, August 2, 2016 2:57 PM
To: Ni, Chaoying {cni-at-udel.edu}

With our CM-12 filaments, I sometimes have to take out and readjust new filaments after they have initially been installed. The filament alignment can change too much with initial heating to adjust it with just the shift x,y knobs. Also, I would try manually adjusting the filament a little further back from the aperture, and then using the emission settings to bring it closer as you do alignment. Sometimes wI find our filaments are set a little too close to the aperture.


Crystal Replogle
TEM Technical Manager
Asbestos TEM Laboratories

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Email: researchers4u-at-gmail.com
Name: Sanaz shobeikeh

Organization: Shiraz university, iran

Title-Subject: [Filtered] Transmission electron microscope cm-10 alignment, beam disappear in M magnification range with object aperture in
Message: Hi:
Sorry to bother you i just have replaced the cm-10 tungsten filament and now i'my doing its alignment, but now I have found out that the student that was working with the microscope when it's filament got burnt got panicked and pressed the hard reset small push button with its pen tip. For now i found the beam with gun shift on filament heating of 19 and 20,i have done the gun procedure and got the beam even after i entered C2 aperture. but in higher mags (M range and higher) with obj.
aperture in the beam way, i am facing two obstacles :first i can't move the beam with shift x/y and INT volume , and second when i try to move the beam to the center of big screen with mechanical obj.
x/y controls, it's like something is in its way (something round) something dark that covers center of phosphorus big screen. i was thinking to correct the C2 centration so i become sure this has nothing to do with obj. aperture problem. for now i have no beam intensity in high magnifications (M range and higher) but in low mag range there is a round and green beam. I would appreciate any advice. Best regards
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19, 33 -- Subject: RE: [Microscopy] viaWWW: Advice Needed Transmission electron
19, 33 -- microscope cm-10
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From: microscopy.listserver-at-gmail.com
Date: Tue, 2 Aug 2016 18:59:05 -0500
Subject: [Microscopy] viaWWW:how to prevent section folds

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Email: marie.cantino-at-uconn.edu Name: Marie Cantino

Organization: Dr.

Title-Subject: [Filtered] section folds

Message: Does anyone know how to prevent section folds in 1 or 2 micron thick sections of plant stem
material embedded in Spurrs resin? The sections are fairly large, about 4 x 7 mm, cut on a histo
diamond, then placed on water drops on Fisher Superfrost Plus slides and dried on a slide warmer at
about 70 degrees C. We get multiple section folds parallel to the short axis of the rectangle, that
start at the resin surrounding the tissue and project into the tissue at the center of the block.
Thanks! -Marie

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From: microscopy.listserver-at-gmail.com
Date: Wed, 3 Aug 2016 07:12:06 -0500
Subject: [Microscopy] viaWWW:

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Email: tomw-at-uidaho.edu Name: Tom Williams

Organization: Univ of Idaho

Title-Subject: [Filtered] Simulation of TEM EDS w/DTSA

Message: Greetings,

I have a Faculty member using DTSA to simulate EDS spectra collected with TEM EDS. [using Sim
Alien]. Sim samples are silicate minerals as "grain mounts" on a Cu grid [assumed config]. We are
working our way through the Preferences set up for instrument, detector, sample. Using the general
parameters of my TEM SDD detector [Thermo SDD] and using 100 & 200 kV acc voltages.
Question: [ignoring the somewhat steep DTSA learning curve] are there specific issues or problems
with using DTSA to simulate TEM EDS spectra? Assuming anyone out there does this kind of thing!

Any sage advice would be appreciated.

Thanks,
Tom


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From: microscopy.listserver-at-gmail.com
Date: Wed, 3 Aug 2016 18:35:11 -0500
Subject: [Microscopy] viaWWW:Embedding gram positive bacteria for sectioning

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Email: joseph.uknalis-at-ars.usda.gov Name: Joe Uknalis

Title-Subject: [Filtered] Embedding gram positive bacteria for sectioning

Message: I had a batch of gram positive bacteria that I embedded in Medium hardness EM Sciences
Spurr's resin. I find most bacteria have holes despite long infiltration under vacuum.

Would a softer mix infiltrate better? Or is there a better resin for gram positive bacteria?

thanks

Joe


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From: microscopy.listserver-at-gmail.com
Date: Wed, 3 Aug 2016 18:36:00 -0500
Subject: [Microscopy] viaWWW:Olympus Fluoview 500 controller card

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Email: gncherr-at-ucdavis.edu Name: Gary Cherr

Organization: UC Davis Bodega Marine Lab

Title-Subject: [Filtered] need Olympus Fluoview 500 controller card

Message: We have an Olympus Fluoview 500 scanning laser confocal microscope running on WinXP. The
controller card (2 PCI cards linked together) has failed. Does anybody know where I can purchase
one? Olympus is looking but not supported anymore.

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From: protrain-at-emcourses.com
Date: Thu, 4 Aug 2016 04:52:35 -0500
Subject: [Microscopy] FW: viaWWW: Advice Needed Transmission electron microscope cm-10

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

Sorry to see your problems but maybe I can help. After 50 years with these
instruments the problem you have I have seen very many times, I hope we can
fix it for you?
Set out below are the very basic principles for alignment of a TEM.

1. All lenses OFF, all apertures out and specimen out
2. High voltage on, filament heated to a position just short of the
position that you would expect to be normal (de saturated source is bigger
than a saturated source so often easier to find)
3. With gun alignment shift controls search for the beam. (if this is
difficult use a raster motion of stepping one control in small steps and
each step take the other control through its full range.
4. Once the beam is found adjust the gun tilt to obtain a maximum
signal.
5. Switch on the first condenser lens, set the lens to a large spot
size and adjust the gun tilt and shift for a maximum signal.
6. Switch on the second condenser lens and adjust Condenser 2 (often
called brightness) to obtain the brightest spot and centre this with you
illumination shift controls.
7. Switch on the objective lens and adjust Condenser 2 and the
illumination shift controls again.
8. Set the magnification around 2,000X and switch on each of the other
imaging lenses. Adjust Condenser 2 and the illumination shift controls to
obtain the maximum intensity.
9. Switch to diffraction and obtain the diffraction point, shift this
with the last lens in the column to the centre of the screen. This may be a
mechanical or deflection coil alignment for image centre or equivalent name.
10. Insert a condenser aperture and with the Condenser 2 at focus and
centred, expand the Condenser (clockwise) and adjust the aperture shadow to
fit the screen with the aperture controls. Take the Condenser back to
crossover and centre with the illumination shift if required, then repeat
this section for a constant centre. Always expand the beam by turning the
Condenser clockwise.
11. Adjust Condenser 2 to crossover and you will visualise the virtual
source. Use the gun alignment tilt to set the "spot and halo" to be even on
opposite sides, centre with the gun alignment shift;. Heat the filament
until it is ALMOST a solid spot. (The folk law which says you must over
saturate the filament slightly became out of date about 30 years ago!)
12. Insert a specimen and see that everything is operating near normal.
As you focus you may find the image and the illumination alignment move,
this is to be expected so correct the illumination with the illumination
shift controls. Check section 10 again.
13. Switch on the objective alignment pulse system (I do not remember
what it is called on an EM10), it will make the image pulse to help you find
the objective axis centre. Use the illumination alignment tilt to place the
centre of the pulse on the centre of the screen, correcting illumination
movement with the illumination shift controls.
13. Switch to diffraction and reset the diffraction centre, insert an
objective aperture.

I hope I have thought of everything, if not please come back to be.

Steve

Steve Chapman FRMS
Protrain for Consultancy and Training in Electron Microscopy
+44 (0)7711 606967
web www.emcourses.com




----------------------------------------------------------------------------
----------------
Email: researchers4u-at-gmail.com
Name: Sanaz shobeikeh

Organization: Shiraz university, iran

Title-Subject: [Filtered] Transmission electron microscope cm-10 alignment,
beam disappear in M magnification range with object aperture in
Message: Hi:
Sorry to bother you i just have replaced the cm-10 tungsten filament and now
i'my doing its alignment, but now I have found out that the student that was
working with the microscope when it's filament got burnt got panicked and
pressed the hard reset small push button with its pen tip. For now i found
the beam with gun shift on filament heating of 19 and 20,i have done the gun
procedure and got the beam even after i entered C2 aperture. but in higher
mags (M range and higher) with obj.
aperture in the beam way, i am facing two obstacles :first i can't move the
beam with shift x/y and INT volume , and second when i try to move the beam
to the center of big screen with mechanical obj.
x/y controls, it's like something is in its way (something round) something
dark that covers center of phosphorus big screen. i was thinking to correct
the C2 centration so i become sure this has nothing to do with obj. aperture
problem. for now i have no beam intensity in high magnifications (M range
and higher) but in low mag range there is a round and green beam. I would
appreciate any advice. Best regards
Login Host: 5.216.254.127
Listserver Email Form V - 20120416
---------------------------------------------------------------------------



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{microscopylistserver-noreply-at-microscopy.com}
10, 53 -- Subject: viaWWW: Advice Needed Transmission electron microscope
cm-10 10, 53 -- alignment, beam disappear in M magnification range with
object aperture in 10, 53 -- References:
{201608011529.u71FTD7o025749-at-ns.microscopy.com}
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17, 20 -- From protrain-at-emcourses.com Thu Aug 4 04:52:29 2016
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17, 20 -- Subject: FW: [Microscopy] viaWWW: Advice Needed Transmission electron microscope cm-10
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From: protrain-at-emcourses.com
Date: Thu, 4 Aug 2016 04:54:10 -0500
Subject: [Microscopy] viaWWW: Advice Needed Transmission electron microscope cm-10

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

Sorry to see your problems but maybe I can help. After 50 years with these
instruments the problem you have I have seen very many times, I hope we can
fix it for you?
Set out below are the very basic principles for alignment of a TEM.

1. All lenses OFF, all apertures out and specimen out
2. High voltage on, filament heated to a position just short of the
position that you would expect to be normal (de saturated source is bigger
than a saturated source so often easier to find)
3. With gun alignment shift controls search for the beam. (if this is
difficult use a raster motion of stepping one control in small steps and
each step take the other control through its full range.
4. Once the beam is found adjust the gun tilt to obtain a maximum
signal.
5. Switch on the first condenser lens, set the lens to a large spot
size and adjust the gun tilt and shift for a maximum signal.
6. Switch on the second condenser lens and adjust Condenser 2 (often
called brightness) to obtain the brightest spot and centre this with you
illumination shift controls.
7. Switch on the objective lens and adjust Condenser 2 and the
illumination shift controls again.
8. Set the magnification around 2,000X and switch on each of the other
imaging lenses. Adjust Condenser 2 and the illumination shift controls to
obtain the maximum intensity.
9. Switch to diffraction and obtain the diffraction point, shift this
with the last lens in the column to the centre of the screen. This may be a
mechanical or deflection coil alignment for image centre or equivalent name.
10. Insert a condenser aperture and with the Condenser 2 at focus and
centred, expand the Condenser (clockwise) and adjust the aperture shadow to
fit the screen with the aperture controls. Take the Condenser back to
crossover and centre with the illumination shift if required, then repeat
this section for a constant centre. Always expand the beam by turning the
Condenser clockwise.
11. Adjust Condenser 2 to crossover and you will visualise the virtual
source. Use the gun alignment tilt to set the "spot and halo" to be even on
opposite sides, centre with the gun alignment shift;. Heat the filament
until it is ALMOST a solid spot. (The folk law which says you must over
saturate the filament slightly became out of date about 30 years ago!)
12. Insert a specimen and see that everything is operating near normal.
As you focus you may find the image and the illumination alignment move,
this is to be expected so correct the illumination with the illumination
shift controls. Check section 10 again.
13. Switch on the objective alignment pulse system (I do not remember
what it is called on an EM10), it will make the image pulse to help you find
the objective axis centre. Use the illumination alignment tilt to place the
centre of the pulse on the centre of the screen, correcting illumination
movement with the illumination shift controls.
13. Switch to diffraction and reset the diffraction centre, insert an
objective aperture.

I hope I have thought of everything, if not please come back to be.

Steve

Steve Chapman FRMS
Protrain for Consultancy and Training in Electron Microscopy
+44 (0)7711 606967
web www.emcourses.com




----------------------------------------------------------------------------
----------------
Email: researchers4u-at-gmail.com
Name: Sanaz shobeikeh

Organization: Shiraz university, iran

Title-Subject: [Filtered] Transmission electron microscope cm-10 alignment,
beam disappear in M magnification range with object aperture in
Message: Hi:
Sorry to bother you i just have replaced the cm-10 tungsten filament and now
i'my doing its alignment, but now I have found out that the student that was
working with the microscope when it's filament got burnt got panicked and
pressed the hard reset small push button with its pen tip. For now i found
the beam with gun shift on filament heating of 19 and 20,i have done the gun
procedure and got the beam even after i entered C2 aperture. but in higher
mags (M range and higher) with obj.
aperture in the beam way, i am facing two obstacles :first i can't move the
beam with shift x/y and INT volume , and second when i try to move the beam
to the center of big screen with mechanical obj.
x/y controls, it's like something is in its way (something round) something
dark that covers center of phosphorus big screen. i was thinking to correct
the C2 centration so i become sure this has nothing to do with obj. aperture
problem. for now i have no beam intensity in high magnifications (M range
and higher) but in low mag range there is a round and green beam. I would
appreciate any advice. Best regards
Login Host: 5.216.254.127
Listserver Email Form V - 20120416
---------------------------------------------------------------------------



--
===========================================
Do not reply to this message it is from the Microscopy Listserver NO-REPLY
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============================================

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10, 53 -- Subject: viaWWW: Advice Needed Transmission electron microscope
cm-10 10, 53 -- alignment, beam disappear in M magnification range with
object aperture in 10, 53 -- References:
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17, 20 -- Subject: [Microscopy] viaWWW: Advice Needed Transmission electron microscope cm-10
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From: microscopy.listserver-at-gmail.com
Date: Thu, 4 Aug 2016 09:02:49 -0500
Subject: [Microscopy] viaWWW:October Biological TEM Workshop

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Email: jpshield-at-uga.edu Name: John P Shields

Organization: University of Georgia

Title-Subject: [Filtered] October Biological TEM Workshop

Message: This intensive, three-day workshop will provide a practical and basic theoretical
introduction to the Transmission Electron Microscope and biological sample preparation techniques.
Each day will consist of lecture, discussion and hands-on training led by GEM staff.
What: Anyone requiring training on TEM and biological sample preparation. The workshop will be
limited to 6 participants based on the availability of equipment.
When: Monday, October 24 through Wednesday, October 26, 2016, 8am-5pm each day

Where: Georgia Electron Microscopy Labs, 154 Barrow Hall, University of Georgia, Athens, GA 30602

Registration: Contact John Shields (jpshield-at-uga.edu) for more information and to sign up.
Registration requires iLab account through the GEM website. gem.uga.edu

Deadline: October 15, 2016


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From: WHITTAKS-at-si.edu
Date: Thu, 4 Aug 2016 11:08:51 -0500
Subject: [Microscopy] Bal-Tec/Balzers coater and cpd service

Contents Retrieved from Microscopy Listserver Archives
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Anyone know who is service or providing parts for Bal-Tec equipment? Both my CPD and Med -20 evaporator are shot and require some TLC to remain operational.

Thanks,


Scott Whittaker
Lab Manager
Imaging
P.O. Box 37012 MRC-104 Room W150
Washington, DC 20013-7012
w 202.633.0891 whittaks-at-si.edu

SMITHSONIAN INSTITUTION
NATIONAL MUSEUM OF NATURAL HISTORY





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From: tbargar-at-unmc.edu
Date: Mon, 8 Aug 2016 09:18:25 -0500
Subject: [Microscopy] Glow discharge systems

Contents Retrieved from Microscopy Listserver Archives
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I would like to hear privately from anyone who has a Pelco easiGlow Dual Chamber Glow Discharge system or has a EMS GLOQUBE-D Dual Camber Glow Discharge System. I would like to know your experiences with either one of these systems.

Tom Bargar
UNMC
Electron Microscopy Core Facility
986395 Nebraska Medical Center
Omaha, NE 68198-6395
402-559-7347
tbargar-at-unmc.edu


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From: nicholas.conoan-at-unmc.edu
Date: Mon, 8 Aug 2016 11:39:56 -0500
Subject: [Microscopy] Need advice to optimize MiniSOG assay

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Hello all:

Our lab is trying to optimize the reaction conditions for the photooxidation of DAB using the MiniSOG construct, but we are encountering difficulties getting a good electron-dense reaction product to form. Does anyone have first-hand experience working with the MiniSOG construct, or even analyzing samples for others who have done this reaction?

Currently, we are transfecting HEK 293-T cells or HeLa cells with 200ng of plasmid using the FuGENE transfection reagent on Thermanox coverslips in 6-well cell culture dishes. We have been examining the fluorescent signal before fixation, and the plasmid that gives us the most promising signal is a peroxisome-localized MiniSOG/mCherry fusion. Therefore, we are focusing on optimizing peroxisome labeling. We are doing a 30 minute fix in 2% glutaraldehyde in 0.1M cacodylate buffer, 3 x 5min buffer washes, then a 1 hour blocking in 50mM glycine, 10mM KCN, 5mM aminotriazole (buffered) followed by 3 x 5 min buffer washes. The light exposure is being performed on a Leica wide-field inverted microscope using the EL6000 light source, which has a 120W metal halide bulb from what I can find online. We are going through the 20x long working distance objective (I don't have the NA handy), and exposure is 2-5 minutes/FOV. Oxygen is being supplied by a portable tank and is both bubbled into the DAB solution beforehand and passed over the well during exposure.

Right now the fluorescent signal looks good, but when we are finished processing and embedding, there is little to no electron-dense product in the thin sections. Any suggestions are welcome.

Thank you,
Nick

Nicholas Conoan
Electron Microscopy Specialist
University of Nebraska Medical Center
Wittson Hall 2013
402-559-7347


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From: microscopy.listserver-at-gmail.com
Date: Mon, 8 Aug 2016 19:19:36 -0500
Subject: [Microscopy] viaWWW: Gatan EELS Training in October 2016

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Email: jhyun-at-gatan.com Name: John Hyun

Organization: Gatan

Title-Subject: [Filtered] EELS Training in October 2016

Message: EELS & EFTEM Analysis Training School October 2016
October 11-14, 2016
Gatan R&D Headquarters
Pleasanton, CA USA

Electron energy loss spectroscopy (EELS) is a powerful technique that provides both compositional
and chemical information from sub-nanometer areas in the sample. As a course attendee, you will
learn best practices to set up and optimize your EELS hardware and experimental protocols so you can
capture and extract the maximum amount of compositional and chemical information from your TEM
samples. Topics include:

-Fundamentals of EELS and energy-filtered imaging in TEM
-Principles of operation of EFTEM and EELS systems
-Optimization of EFTEM and EELS data acquisition
-Quantification of elemental composition
-Other information provided by EFTEM/EELS and how best to extract it
-Use of EELS signals to form maps of elemental and chemical composition
-EFTEM and STEM EELS spectrum imaging techniques
-Identification of material phases via EELS fine structure mapping
-Applications to biological and physical science specimens

Register online: http://www.gatan.com/company/events/eels-eftem-analysis-training-school-october-2016

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From: microscopy.listserver-at-gmail.com
Date: Mon, 8 Aug 2016 19:20:18 -0500
Subject: [Microscopy] viaWWW: Immunogold Negative staining of Exosomes

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Email: yankova-at-uchc.edu Name: Maya Yankova

Organization: University of Connecticut Health Center

Title-Subject: [Filtered] Immunogold Negative staining of Exosomes

Message: Dear All,
We have a researcher who is interested in immunogold labeleing and negative staining of exosomes
isolated from Mouse macrophages.So far we have done immunogold and negative staining with CD63
rabbit from SBI and didn't get any labeling. I would like to ask you, if you can recommend other
companies we can get exosomal markers : CD63,CD9 etc. and do immunogold negative staining on whole
mount exosomes.
Thank you in advance for your help!

Maya Yankova,
Research Associate
Central Electron Microscopy Facility
Department of Molecular, Microbial & Structural Biology
University of Connecticut Health Center
263 Farmington Avenue
Farmington, CT 06030-3305
ph: 860.679.2395
yankova-at-neuron.uchc.edu


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From: microscopy.listserver-at-gmail.com
Date: Mon, 8 Aug 2016 19:20:55 -0500
Subject: [Microscopy] viaWWW:Job opening: TEM electron microscopy

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Email: chiyen-at-uw.edu Name: Chiyen Miller

Organization: University of Washington

Title-Subject: [Filtered] Job opening: TEM electron microscopy

Message: University of Washington Medical Center Anatomic Pathology has a job opening for TEM
electron microscopy. For inquiries please go to the University of Washington employment
opportunities website and under find a job, search the req #124675. Thank you!

Chiyen Miller
Lead, EM Lab
UWMC

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From: microscopy.listserver-at-gmail.com
Date: Mon, 8 Aug 2016 19:21:32 -0500
Subject: [Microscopy] viaWWW:Correlative Light and Electron Microscopy

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Email: ricardo.vilela-at-leica-microsystems.com Name: Ricardo Vilela

Organization: Leica Microsystems

Title-Subject: [Filtered] Correlative Light and Electron Microscopy

Message: Hi everyone,
Have you ever tried the CLEM software of TESCAN, named AURORA?

I would like to hear your comments about this system, functionality & benefits.

Thanks a Lot.

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From: microscopy.listserver-at-gmail.com
Date: Mon, 8 Aug 2016 19:22:09 -0500
Subject: [Microscopy] viaWWW:EM/XRM Specialist position with Carl Zeiss

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Email: john.wailes-at-zeiss.com Name: John Wailes

Organization: Carl Zeiss

Title-Subject: [Filtered] EM/XRM Specialist position with Carl Zeiss

Message: Carl Zeiss Microscopy is looking for a Life Sciences, EM/XRM systems specialist, to cover a
territory in the eastern half of the United States. If you are interested, please contact John
Wailes using the details below.

John Wailes
Carl Zeiss Microscopy, LLC
Systems Sales Manager, Academia EM/XRM
Mobile: (845) 553-5339
john.wailes-at-zeiss.com


YOUR JOB

* Selling and support of ZEISS instrumentation and related EM/XRM components.

* Support customers with products and applications for EM/XRM microscopes in the defined territory
for all markets.

* Sample prepping and presentation of EM/XRM microscopy imaging applications.

* Generation of EM/XRM sales opportunities.

* Perform presentations of ZEISS products at demonstrations, meetings, seminars, and workshops.

* Completion and submission of the required forms for each qualifying order.

* Drive EM/XRM sales opportunities to close.

OUR REQUIREMENTS

* Bachelor degree in life sciences, engineering, or physical sciences preferred from an accredited
college of university.

* Positive can do attitude coupled with a willingness to acquire product knowledge independently.

* Valid driver's license and regular local, national, and international travel required.

* Must be able to lift up to 50lbs of equipment.

With just under 25,000 employees, ZEISS is one of the global leaders in the optical and
optoelectronic industries and has been contributing to technological progress for over 165 years.
ZEISS develops and distributes lithography optics, measuring technology, microscopes, medical
technology, eyeglass lenses, camera and cine lenses, binoculars and planetarium technology.

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From: microscopy.listserver-at-gmail.com
Date: Mon, 8 Aug 2016 19:22:44 -0500
Subject: [Microscopy] viaWWW:Lego Ideas: SEM, TEM, XRD

Contents Retrieved from Microscopy Listserver Archives
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Email: see.wee.chee-at-gmail.com Name: See Wee

Title-Subject: [Filtered] Lego Ideas: SEM, TEM, XRD

Message: Let's all vote for this guy and make these into real Lego products. :)

https://ideas.lego.com/projects/102281

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From: microscopy.listserver-at-gmail.com
Date: Wed, 3 Aug 2016 11:58:38 +0000
Subject: [Microscopy] viaWWW:how to prevent section folds

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Email: marie.cantino-at-uconn.edu Name: Marie Cantino

Organization: Dr.

Title-Subject: [Filtered] section folds

Message: Does anyone know how to prevent section folds in 1 or 2 micron thick sections of plant stem material embedded in Spurrs resin? The sections are fairly large, about 4 x 7 mm, cut on a histo diamond, then placed on water drops on Fisher Superfrost Plus slides and dried on a slide warmer at about 70 degrees C. We get multiple section folds parallel to the short axis of the rectangle, that start at the resin surrounding the tissue and project into the tissue at the center of the block.
Thanks! -Marie

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From: jehrman-at-mta.ca
Date: Tue, 9 Aug 2016 06:27:58 -0500
Subject: [Microscopy] Re: viaWWW:Lego Ideas: SEM, TEM, XRD

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




-------- Forwarded Message --------




-------- Forwarded Message --------

Well, they don't have the correct graphics on some of the controls, but
years ago based on a challenge to my son, he made me these out of his
own Lego stash:

http://www.mta.ca/dmf/lego.html

Somewhat sadly, many visitors to the lab find these more interesting
than the actual instruments! Sigh.

Jim


On 08/08/2016 9:23 PM, microscopy.listserver-at-gmail.com wrote:
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--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
63B York St.
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/dmf

I before E except after C?
protein
counterfeit
albeit
kaleidoscope
reveille
obeisance
Rottweiler
caffeine


==============================Original Headers==============================
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From: colijn.1-at-osu.edu
Date: Tue, 9 Aug 2016 09:38:36 -0500
Subject: [Microscopy] viaWWW:

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Tom,

I have used DTSA for modeling S/TEM spectra using the MC software. I'm
not sure I would use the software for high precision work, but it should
be OK for things like detection limits, etc.

There are 2 main differences between TEM and SEM modeling: beam voltage
and the thin film sample. DTSA allows for a thin film sample where you
can set the substrate to nothing. The kV should affect only the
ionization cross-section. Once the overvoltage gets above 4-5, there is
a relatively slow variation in the cross-section. (see for example,
Williams & Carter 2d ed fig 4.4). Raynald Gauvin's work (M&M 2012
#1000) suggests that there should be little difference in the models at
the higher kVs. The agreement of the models gives me a bit more
confidence that the cross-sections are meaningful.

You can always collect spectra from known samples and compare them to
the MC models and see how close they come.

If anyone has more detailed info about the modeling validity, I'd like
to know too!

Cheers,
Henk



--------------------

Hendrik O. Colijn
Center for Electron Microscopy and AnalysiS
The Ohio State University
1305 Kinnear Rd, Suite 100, Columbus, OH 43212

colijn.1-at-osu.edu 614/643-3458
cemas.osu.edu {about:cemas.osu.edu}

"Time is that quality of nature which keeps things from happening all at
once." (Ray Cummings - 1922)
Lately it doesn't seem to be working.



------ Original Message ------
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To: colijn.1-at-osu.edu
Sent: 8/3/2016 8:42:15 AM



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From: microscopy.listserver-at-gmail.com
Date: Wed, 10 Aug 2016 07:07:35 -0500
Subject: [Microscopy] viaWWW: Immunogold Negative staining of Exosomes

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X-from: Lee Cohen-Gould {lcgould-at-med.cornell.edu}
To: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} , yankova-at-uchc.edu
{yankova-at-uchc.edu}



Hi Maya
The gold labeling will only work if the epitope is on the external side of the exosome membrane.
Remember, they are like tiny balloons and only the external surface is exposed to the antibody.
Not a solution, just something to consider.
Lee

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Email: yankova-at-uchc.edu Name: Maya Yankova

Organization: University of Connecticut Health Center

Title-Subject: [Filtered] Immunogold Negative staining of Exosomes

Message: Dear All,
We have a researcher who is interested in immunogold labeleing and negative staining of exosomes
isolated from Mouse macrophages.So far we have done immunogold and negative staining with CD63
rabbit from SBI and didn't get any labeling. I would like to ask you, if you can recommend other
companies we can get exosomal markers : CD63,CD9 etc. and do immunogold negative staining on whole
mount exosomes.
Thank you in advance for your help!

Maya Yankova,
Research Associate
Central Electron Microscopy Facility
Department of Molecular, Microbial & Structural Biology
University of Connecticut Health Center
263 Farmington Avenue
Farmington, CT 06030-3305
ph: 860.679.2395
yankova-at-neuron.uchc.edu


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From: microscopy.listserver-at-gmail.com
Date: Wed, 10 Aug 2016 07:08:02 -0500
Subject: [Microscopy] viaWWW:how to prevent section folds

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X-from: Cantino, Marie {marie.cantino-at-uconn.edu}
To: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com}



We have tried this technique with dichloroethylene without success. I think the sections are really too big, but we have gotten some other suggestions that have reduced (but not eliminated) the folds. Thanks for your suggestion!

Sent from my iPad

} On Aug 8, 2016, at 10:43 PM, "microscopy.listserver-at-gmail.com" {microscopy.listserver-at-gmail.com} wrote:
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} Subject: RE: [Microscopy] viaWWW:how to prevent section folds
} Date: Wed, 3 Aug 2016 11:58:38 +0000
} X-from: Joachim Siegmund {jsiegmund-at-7thwavelabs.com}
} To: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com}
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}
} Did you try Ether on a Q-Tip, hovering over the section on the water drop?
}
} Regards,
}
} Joachim Siegmund
} Image Analyst
} Seventh Wave Laboratories LLC
} 19 Worthington Access Drive
} Maryland Heights, MO 63043
} jsiegmund-at-7thwavelabs.com
} www.7thwavelabs.com
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} X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com]
} Sent: Tuesday, August 02, 2016 10:49 PM
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} Subject: [Microscopy] viaWWW:how to prevent section folds
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} Email: marie.cantino-at-uconn.edu Name: Marie Cantino
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} Organization: Dr.
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} Title-Subject: [Filtered] section folds
}
} Message: Does anyone know how to prevent section folds in 1 or 2 micron thick sections of plant stem material embedded in Spurrs resin? The sections are fairly large, about 4 x 7 mm, cut on a histo diamond, then placed on water drops on Fisher Superfrost Plus slides and dried on a slide warmer at about 70 degrees C. We get multiple section folds parallel to the short axis of the rectangle, that start at the resin surrounding the tissue and project into the tissue at the center of the block.
} Thanks! -Marie
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From: microscopy.listserver-at-gmail.com
Date: Wed, 10 Aug 2016 07:10:52 -0500
Subject: [Microscopy] viaWWW:Job Opening: Scanning Electron Microscopist

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From: nizets2-at-yahoo.com
Date: Thu, 11 Aug 2016 05:41:12 -0500
Subject: [Microscopy] Re: viaWWW: Immunogold Negative staining of Exosomes

Contents Retrieved from Microscopy Listserver Archives
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Joseph,
You can also use a 1:1 mixture of the Epon and Spurr's resin. Use
only half the concentration of each catalyst (0.75% of DMP-30 and DMAE).
Use extended parts of your transition solvent to resin (30-50-90% in
resin-2-3 hrs./overnight rocking ), then when you get to pure changes use a
15 psi vacuum for at least one of these pure resin changes. I then add an
extra day of pure resin changes. I agree with Lou Ann that you must mix and
centrifuge the pellet for each change. I have found that some bacteria will
Infiltrate well while others (KO) may not. Good luck.

Sincerely,
Michael Delannoy

-----Original Message-----
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Although the remark is generally true, when it comes to CD it only makes sense if it labels external epitopes.
For this very reason I would suggest to do pre-embeddings labeling.
Also you may consider a positive control to control if you are able to label the marker when you are sure that one is present.

regards
Stephane
--------------------------------------------
On Wed, 8/10/16, microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} wrote:

Subject: [Microscopy] viaWWW: Immunogold Negative staining of Exosomes
To: nizets2-at-yahoo.com
Date: Wednesday, August 10, 2016, 2:18 PM




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X-from:     Lee Cohen-Gould {lcgould-at-med.cornell.edu}
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yankova-at-uchc.edu
{yankova-at-uchc.edu}



Hi Maya
The gold labeling will only work if the epitope is on the
external side of the exosome membrane. 
Remember, they are like tiny balloons and only the external
surface is exposed to the antibody.
Not a solution, just something to consider.
Lee

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Subject: [Microscopy] viaWWW: Immunogold Negative staining
of Exosomes




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Email: yankova-at-uchc.edu
Name: Maya Yankova

Organization: University of Connecticut Health Center

Title-Subject: [Filtered] Immunogold Negative staining of
Exosomes

Message: Dear All,
We have a researcher who is interested in immunogold
labeleing and negative staining of exosomes
isolated from Mouse macrophages.So far we have done
immunogold and negative staining with CD63
rabbit from SBI and didn't get any labeling.  I would
like to ask you, if you can recommend other
companies we can get  exosomal markers : CD63,CD9
etc.  and do immunogold negative staining on whole
mount exosomes.
Thank you in advance for your help!

Maya Yankova,
Research Associate
Central Electron Microscopy Facility
Department of Molecular, Microbial & Structural Biology
University of Connecticut Health Center
263 Farmington Avenue
Farmington, CT 06030-3305
ph: 860.679.2395
yankova-at-neuron.uchc.edu


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From: tbargar-at-unmc.edu
Date: Mon, 15 Aug 2016 14:34:58 -0500
Subject: [Microscopy] TEM of Cells grown in hydrogel

Contents Retrieved from Microscopy Listserver Archives
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Name: Alexandra Khudoleeva
School: Texas A&M University
Grade/Education Level: Graduate
Location: Albuquerque, NM
US
Email: alexa134-at-gmail.com

Does anyone have a protocol for processing Cells grown in hydrogel for TEM, that will not shrink the hydrogel?

Tom Bargar
UNMC
Electron Microscopy Core Facility
986395 Nebraska Medical Center
Omaha, NE 68198-6395
402-559-7347
tbargar-at-unmc.edu


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6, 36 -- Subject: TEM of Cells grown in hydrogel
6, 36 -- Thread-Topic: TEM of Cells grown in hydrogel
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From: microscopy.listserver-at-gmail.com
Date: Mon, 15 Aug 2016 20:46:10 -0500
Subject: [Microscopy] viaWWW:Thanks - How to prevent section folds

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Email: Marie.cantino-at-uconn.edu Name: Marie Cantino

Organization: University of Connecticut, Biosciences Electron Microscopy Laboratory

Title-Subject: [Filtered] how to prevent section folds

Message: Thanks to the MANY people who took time to respond to my question about section folds.
Unfortunately the person who is actually testing these tips is on vacation, so I can't comment yet
on which ones worked best for us, but since several people requested a summary of responses, I have
organized them below.
FIXATION AND EMBEDDING
1. Increase fixation time. If folds are at the centers of the sections there may be a change in
tissue fixation (fixed at the periphery but not at center). 2. Use long infiltration times,
especially if cell walls are thick. For resin-solvent steps and first 100% step, use Spurrs WITHOUT
catalyst (overnight) followed by two changes of 100% Spurrs WITH catalyst for a full day.
3. Try matching the resin hardness to the tissue hardness by using a different resin mixture.

TRIMMING
4. Make razor blade cuts in the embedding media around the sample then mount sections in the
embedding medium to make the cuts disappear (not clear whether the respondent had tried this)
5. Make the block face smaller!! [the block face seemed very large to me, but I’m an electron
microscopist so anything larger than 1 mm seems large. However, it was helpful to get conformation
that this is large even for semithin sections].
6. Trim a trapezoid, not a rectangle.
7. Trim the tissue at the bottom edge or on all sides to eliminate empty resin

MICROTOMY
8. Try cutting sections thinner, 0.4-0.5 microns, and stain with 1% toluidine blue stain.
9. Try cutting sections thicker.
10. Apply solvent vapors to sections still in the knife boat by waving a Q-tip or sliver of filter
paper soaked with solvent over the sections. Solvents recommended were chloroform or ether (we have
also used trichloroethylene). Note toxicity of these solvents by inhalation!
11. Use a heat pen to achieve the same effect as above.
12. Wet a plastic bottle cap with chloroform and hold the cap over the boat as the vapors
evaporated. Chloroform vapor is heavier than air, and creates a downward force on the surface of
water in your boat.
PICK-UP
13. Pick sections up and float them on a container of hot water to stretch them before transferring
them to the slide to dry them. 14. Pick sections up with a loop (e.g., Perfect Loop) and immerse
them in the water droplet rather than trying to roll the loop over to get the sections onto the
slide. Come straight down on top of the water droplet with the loop (section side up) and let the
sections float off of the loop and onto the water surface.
15. Add ethylene glycol (maybe 1%?) or acetone ( { 10%?) to the water drop on the slide (not in the
trough). However, be aware that section dimensions may change. One respondent also suggested
adding ethanol at this step, but had not tried this with plastics.
16. Switch to untreated glass slides. Superfrost Plus slides have a surface treatment that will make
sections adhere more readily, not allowing them to spread out as they dry down and causing section
puckering.

DRYING
17. Put a small open glass Petrie dish on the hot plate. Heat it. Put the slide, drip side down over
the dish to evaporate. If necessary, flip dry slide over and heat to a higher temp to promote adhesion.
18. Apply solvent vapors (chloroform, xylene) while drying the sections on the slide warmer.
Several suggestions of how to do this were to place a drop of solvent in the lid of a petri dish
over the sections while drying or to hover a chloroform soaked Q-tip over slide during drying
19. Lower the temperature on the slide warmer so that sections will dry VERY slowly, then reheat
after all water is gone to improve adhesion
20. Raise the temperature on the slide warmer slightly then gently agitate the slide while it is on
the hotplate to evenly dry the water.
21. Place a few drops of acetone on a Q-tip on the frosted end of your slide, cover your slide with
a Petri dish and allow the water to dry down on your hotplate. The increased pressure in the dome
produced by the Petri dish from the evaporated acetone helps your sections to dry flat. Have the
hotplate set hot enough to boil water. 22. Heat a small open glass Petri dish on the hot plate. Put
the slide, drip side down over the Petri dish during drying.
23. Look at
https://www.researchgate.net/publication/19412292_Flat_Adherent_Well-Contrasted_Semithin_Plastic_Sections_for_Light_Microscopy


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From: microscopy.listserver-at-gmail.com
Date: Tue, 16 Aug 2016 07:33:46 -0500
Subject: [Microscopy] viaWWW: Training an EM tech from zero experience

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Email: Desertrat99-at-verizon.net Name: Eric Rosen

Organization: Ucla

Title-Subject: [Filtered] Training an EM tech from zero experience.
Message: Howdy all,
I was curious to know how difficult is it to train someone to be an TEM tech when starting at zero
experience?
Thanks



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From: microscopy.listserver-at-gmail.com
Date: Thu, 18 Aug 2016 20:10:09 -0500
Subject: [Microscopy] viaWWW:TEM Open Position Technical Staff NCEM, Berkeley, CA

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Email: kbustillo-at-lbl.gov Name: Karen Bustillo

Organization: NCEM, Molecular Foundry, LBNL

Title-Subject: [Filtered] TEM Open Position Technical Staff NCEM,
Berkeley, CA

Message: The National Center for Electron Microscopy (NCEM), at the
Molecular Foundry, Lawrence Berkeley National Lab is seeking candidates
to fill an open position as a member of the technical staff. The
Molecular Foundry is a DOE funded User Facility.

The technical staff position is in the area of user support for TEM
sample preparation and in situ TEM operation. We are looking for an
outstanding candidate to support and contribute to the user and science
programs at the Molecular Foundry. We are specifically encouraging all
qualified women and candidates from underrepresented groups to apply.
The link to the job posting is below:

https://lbl.taleo.net/careersection/2/jobdetail.ftl?lang=en&job=82803

Questions can be addressed to Andy Minor aminor-at-lbl.gov or Karen
Bustillo kbustillo-at-lbl.gov. Applications will first be reviewed on Aug. 23.

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From: microscopy.listserver-at-gmail.com
Date: Sat, 20 Aug 2016 07:10:16 -0500
Subject: [Microscopy] viaWWW:SEM instrument tech job, CWU

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Email: mattinson-at-Geology.cwu.EDU Name: Chris Mattinson

Organization: Central Washington University

Title-Subject: [Filtered] SEM instrument tech job, CWU

Message: Hi All,

We're currently searching for an instrument tech whose primary charge will be our new SEM, and would
appreciate your help to forward this ad to qualified potential applicants. Screening begins Sept. 1.

Chris

Engineering Technician 3 - Geological Sciences Department at Central Washington University (CWU)

The Engineering Technician 3 oversees the Geological Sciences Department’s major instrumentation and
associated laboratory facilities in the new science building at Central Washington University. This
oversight includes: design and modification of systems and peripherals to achieve desired geological
applications; maintenance, operation and repair of instruments; training users; and overseeing
safety protocol. This position oversees the operation and maintenance of a FEI Quanta 250FEG
scanning electron microscope (SEM) with users from multiple departments at CWU. The position will
also oversee other instruments in the Geological Sciences instrumentation laboratory. For more
information and to apply, see https://careers.cwu.edu (Job ID 729). CWU is an AA/EEO/Title
IX/Veteran/Disability Employer.

__
Chris Mattinson
Associate Professor & Graduate Program Coordinator
Department of Geological Sciences
Central Washington University
400 E. University Way, Ellensburg, WA 98926-7418
Tel: 509.963.1628


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From: microscopy.listserver-at-gmail.com
Date: Tue, 23 Aug 2016 20:06:26 -0500
Subject: [Microscopy] viaWWW: TEM fluorescent screen metal plate stuck at 90 degrees

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Email: the88gtu-at-yahoo.com Name: Joel Leyva

Organization: SJDC Graduate

Title-Subject: [Filtered] TEM fluorescent screen metal plate stuck at 90 degrees

Message: Hello fellow microscopists,
I have a problem with a JEOL JEM-CX II. I replaced the binoculars and attempted to view the sample
on the fluorescent screen but when the plate was moved to the 45 degree it wouldn't go back down. I
then pushed the 90 (vertical) to see if it will go back that way but it got stuck. I could hear a
motor running for 20 seconds before it died off. I can personally send images so you can see what is
meant by fluorescent plate. Thank you.

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From: microscopy.listserver-at-gmail.com
Date: Wed, 24 Aug 2016 07:22:47 -0500
Subject: [Microscopy] viaWWW:Wanted: microtome

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Email: johanna.hoog-at-gu.se Name: Johanna Hoog

Organization: University of Gothenburg

Title-Subject: [Filtered] Wanted: microtome

Message: Dear all,
I am starting a EM lab from scratch here in Gothenburg and would like to ask you if anyone has a
used microtome that functions but is no longer used in your labs? I would pay for transport and if
it is a newer version I could also pay something for the machine itself. Please contact me with info.
Many thanks in advance!

Johanna Hoog
Assistant professor Department of Chemistry and Molecular Biology
University of Gothenburg


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From: wim.hagen-at-me.com
Date: Fri, 26 Aug 2016 06:18:04 -0500
Subject: [Microscopy] position for experienced electron microscopist at MRC-LMB

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http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Exciting opportunity for an experienced electron microscopist to join MRC-LMB Cambridge, UK.

This position provides a rare opportunity for an experienced researcher or engineer to combine laboratory/technical management with research or technical development. You will undertake independent scientific or technical research in the area of cryo-electron tomography and subtomogram averaging, and if appropriate, FIB milling. You will also implement methods and instrumentation for cryo-electron tomography, helping to maintain, develop and support cryo-electron tomography capabilities at the LMB.

The EM facility at LMB currently includes two Titan Krios microscopes, Polara, Scios dual-beam, two 200kV FEG microscopes and 3 T12/Spirit microscopes. In addition, the EM facility has a suite of associated equipment for sample preparation. You will be a member of the lab of John Briggs, which is currently based at the EMBL in Heidelberg, but will be based at the LMB from Jan 2017. Information about the Briggs group is available here: http://www.embl.de/research/units/scb/briggs/index.html

More information about the job is available here: mrc.io/2bzQOR1
The closing date for applications is 18th September.

Feel free to get in touch with John Briggs if you have questions. john.briggs-at-embl.de

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From: Duane.Harland-at-agresearch.co.nz
Date: Fri, 26 Aug 2016 18:54:30 -0500
Subject: [Microscopy] Early career fibre structure scientist position in New Zealand

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Dear list,

We currently have a permanent position available for a structure-oriented early career scientist at AgResearch Lincoln in the South Island of New Zealand.

Full details here
https://careers.sciencenewzealand.org/jobdetails/ajid/r7WV7/Fibre-Structural-Biology-Scientist,18510.html

Our research is often uses a variety of techniques, and the substrates involve both soft tissue (e.g., skin and hair follicles) as well as keratin fibres (which are effectively a complex nano-composite biomaterial). While we don't expect applicants to be experts on keratin fibres or follicle biology, we are looking for someone who can fit into this role easily and brings good experimental design capability and penchant for technical/instrumentation/analysis innovation.

Queries should be directed via the web page above.

Kind regards
Duane

____________________________
Dr Duane P Harland
Senior Scientist
T +64 3 321 8710
E duane.harland-at-agresearch.co.nz
AgResearch Limited
Lincoln Research Centre
Cnr Springs Rd & Gerald Street, Lincoln
Private Bag 4749 Christchurch 8140, New Zealand
T +64 3 325 9900 F +64 3 325 9946 www.agresearch.co.nz
Farming Food and Health. First
Te Ahuwhenua Te Kai me te Whai Ora. Tuatahi

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Attention: The information contained in this message and/or attachments from AgResearch Limited is intended only for the persons or entities to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipients is prohibited by AgResearch Limited. If you have received this message in error, please notify the sender immediately.
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From: microscopy.listserver-at-gmail.com
Date: Sat, 27 Aug 2016 13:41:54 -0500
Subject: [Microscopy] viaWWW:Auxiliary Gas for FEI Quanta 200 (older version)

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Email: kpseverin-at-alaska.edu Name: Ken Severin

Organization: U Alaska Fairbanks

Title-Subject: [Filtered] Auxiliary Gas for FEI Quanta 200 (older version)

Message: I'd like to run with nitrogen at in the low vac (1 Torr) range but am having a heck of a
time getting a stable pressure, with the result being constant changes is the image brightness. If
anyone has a solution or a regulator setup that works I'd like to know about it so I can "copy exact."

The system is perfectly stable when using water vapor so I don't think it is a problem with the
instrument.

Thanks all!

Ken Severin
University of Alaska Fairbanks

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From: wesaia-at-iastate.edu
Date: Sat, 27 Aug 2016 15:43:05 -0500
Subject: [Microscopy] viaWWW:Auxiliary Gas for FEI Quanta 200 (older version)

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Hello, Ken.

We have a Quanta FEG 250 from about 6 years ago. We have the nitrogen vent line teed into the auxiliary gas feed. We have the two-stage regulator set to 1 psi or less. We don't have troubles as you describe. You didn't mention what pressure you have set. I would wonder if the regulator itself has gotten flaky. I would try changing it out for another. I also trust that your nitrogen tank is not near empty.

I can certainly relate to what you mention about the brightness being very sensitive to the pressure level. We've done some work with the Peltier stage on ours and found that we are better off controlling the temperature to change the humidity and get condensation than trying to control pressure. The temperature is better controlled and does not effect brightness.

I can't say I know how FEI runs their control system. It seems they could get by with a single control valve and just choose between the gas source. If they do, then I suppose the variability using N2 could be due to a leak in the tubing somewhere or a leaky solenoid valve. I would probably leave that up to an engineer to troubleshoot.

We had a different brand of VP-SEM that used to have trouble settling in on the set pressure. We could watch the control servo keep cranking the needle valve back and forth. It never settled down. To me, that suggested a control circuit issue - that the system was overcontrolling. Indeed, an intrepid service engineer was able to change out a component in the control board to get more ideal control. He no longer had to come back yearly to replace the valve when it seized up from the constant turning.

Hope it helps some,
Warren Straszheim
Materials Analysis and Research Lab
Iowa State Univeristy

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Email: kpseverin-at-alaska.edu Name: Ken Severin

Organization: U Alaska Fairbanks

Title-Subject: [Filtered] Auxiliary Gas for FEI Quanta 200 (older version)

Message: I'd like to run with nitrogen at in the low vac (1 Torr) range but am having a heck of a time getting a stable pressure, with the result being constant changes is the image brightness. If anyone has a solution or a regulator setup that works I'd like to know about it so I can "copy exact."

The system is perfectly stable when using water vapor so I don't think it is a problem with the instrument.

Thanks all!

Ken Severin
University of Alaska Fairbanks

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From: eikonika-at-otenet.gr
Date: Sat, 27 Aug 2016 15:52:35 -0500
Subject: [Microscopy] free SEM filament timer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Friends

My Angel who repairs the electronic failures of my SEM for free, has created
a stopwatch for counting the SEM filament time (we couldn't find anything
similar on the web). The timer is simple and aesthetic and whoever is
interested can download it at:

https://github.com/proteusx/SEM-Timer

Happy New School Year!

yorgos

Dr Yorgos Nikas
Athens Innovative Microscopy
Skra 36 Voula 16673 GREECE

Tel/fax +30 210 8957677
mobile +30 6945 107477
www.eikonika.net www.aim.cat
*************************************





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10, 22 -- References:
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10, 22 -- Subject: free SEM filament timer
10, 22 -- Date: Sat, 27 Aug 2016 23:53:35 +0300
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From: microscopy.listserver-at-gmail.com
Date: Mon, 29 Aug 2016 18:22:45 -0500
Subject: [Microscopy] viaWWW:Project Scientist Series -Electron Microscopy Scientific Staff

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Email: jzheng-at-calit2.uci.edu Name: Jian-Guo Zheng

Organization: UC Irvine

Title-Subject: [Filtered] Project Scientist Series -Electron Microscopy Scientific Staff

Message: Dear Microscopists in Cryo-TEM:
You might see the similar announcement a few months ago. Last announcement that looked for two TEM
specialists resulted in the hiring of one staff member in materials science. Because of policy
reason, the another position for cryo-TEM specialist for biological and soft materials was closed
before the formal review of the candidates. If you are still interested in the TEM scientific staff
position for cryo-TEM specialist for biological and soft materials, please resubmit your
application. Surely, new submission is warmly welcome. Thanks,

Jian-Guo Zheng
IMRI, UCI

Project Scientist Series -Electron Microscopy Scientific Staff

Salary-Commensurate with experience
POSTING DATE: August 22, 2016 closing date: open until filled
DESCRIPTION
Manage the day-to-day operations and help guide the strategic build-out of the new transmission
electron microscopy (TEM) facility at the UC Irvine Materials Research Institute (IRMI). IMRI has a
large and growing user community of over 300 academic and industry users, with advanced materials
characterization instruments that include state-of-the-art TEMs, scanning electron microscopes
(SEMs), focused ion beam (FIB) systems, and a Kratos AXIS Supra surface science instrument (for
details see http://lexi.eng.uci.edu). IMRI has recently built a new TEM facility housing five major
instruments, including four high-end TEMs (Nion UltraSTEM 200 HERMES, JEOL JEM-ARM300CF “Grand ARM,”
JEM-2800, and JEM-2100F Cryo-TEM) and a dual-beam FIB (Tescan GAIA-3 XMH FIB-SEM). Both the Grand
ARM and the 2100F Cryo-TEM will be equipped with Gatan’s K2 cameras. IMRI is also equipped with a
variety of TEM holders and specimen preparation tools.
A TEM scientific staff position for cryo-TEM specialist for biological and soft materials is
available. The successful applicants must hold PhD or equivalent degrees in the areas of Biology,
Medicine, Materials Science, Physics, or Chemistry, and be TEM experts with at least five years of
in-depth, hands-on experience with cryo-TEM imaging, data analysis, biological sample preparation,
and maintenance of TEM equipment. The applicant must have mastery of modern TEM instrumentation and
methods, data collection, analysis, and interpretation. Candidates with experience operating user
facilities and expertise with advanced TEM techniques are of particular interest. Duties include:
operate, maintain, and improve the TEMs; work with the IMRI leadership to identify and obtain
accessories and additional equipment for a phased build-out of the TEM facility; train, supervise,
and work directly with users to carry out research; co-manage all aspects of day-to-day IMRI
operations; participate in teaching and outreach activities; promote IMRI on and off campus; secure
grants, perform original research, attend scientific conferences, and publish scientific research in
peer-reviewed journals.


The University of California, Irvine is an Equal Opportunity/Affirmative Action Employer advancing
inclusive excellence. All qualified applicants will receive consideration for employment without
regard to race, color, religion, sex, sexual orientation, gender identity, national origin,
disability, age, protected veteran status, or other protected categories covered by the UC
nondiscrimination policy.
TO LEARN MORE AND APPLY
Apply by submitting your application to our online RECRUIT system at:
https://recruit.ap.uci.edu/apply/JPF03682




REQUIREMENTS:

DOCUMENTS
Cover Letter
Curriculum Vitae - Your most recently updated C.V.
Statement of Research
Statement of Teaching (Optional)
Statement of Contributions to Diversity - Statement addressing how past and/or potential
contributions to diversity will advance UCI's Commitment to Inclusive Excellence. (Optional)
Misc / Additional (Optional)

REFERENCES
3-5 letters of reference required

HOW TO APPLY
1. Create an ApplicantID
2. Provide required information and documents

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From: microscopy.listserver-at-gmail.com
Date: Wed, 31 Aug 2016 07:46:21 -0500
Subject: [Microscopy] viaWWW:Dehydration/infiltration issues

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Email: buchsmith-at-gmail.com Name: JoAnn Buchanan

Organization: Allen Institute for Brain Science

Title-Subject: [Filtered] Dehydration/infiltration issues

Message: We recently started using a processor for dehydration and initial infiltration steps. We
are processing very large samples-1 to 1.5 mm.
Since trying the processor, we have gotten poorly infiltrated samples, mainly in the center of the
block. This happened even after we doubled the dehydration times.
We use ethanol series followed by acetonitrile as an intermediate and Hard Plus resin for embedding.
We only do a 2p solvent to 1 Part resin in the processor. We don't have this problem with bench
processing.

Since our protocol is 9 days long, a processor for dehydration really helps. I'd really like to be
able to use the processor for this. and infiltration.

Any troubleshooting ideas or suggestions are welcome.
Thanks in advance,

JoAnn

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From: microscopy.listserver-at-gmail.com
Date: Wed, 31 Aug 2016 18:05:45 -0500
Subject: [Microscopy] viaWWW:Strange Pattern on SEM Images

Contents Retrieved from Microscopy Listserver Archives
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X-from: arezoo.zare-at-okstate.edu

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Email: arezoo.zare-at-okstate.edu Name: Arezoo Zare

Organization: Oklahoma State University

Title-Subject: [Filtered] Strange Pattern on SEM Images

Message: Hello everyone,

I have a question about a strange pattern that shows up on some of my SEM images. I have a SiOC
specimen which does not have a good conductivity but since I need the specimen for other tests I do
not want to coat it. As a result the specimen charges up under SEM and sometimes I can clearly see
the zoom window being brighter than other areas. Until now everything seem reasonable but as soon as
I click on auto brightness/contrast button, a strange pattern appears on the image. The observed
pattern is similar to a few intermittent infinity symbols or something that you draw with a
harmonograph. The pattern only shows up on this sample and not on the other ones. I was wondering if
anyone has any idea about the origin of the observed pattern. I was not able to attach the image
here but I am whiling to share it via e-mail. Thank you for your attention.

My best,
Arezoo

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Listserver Email Form V - 20120416
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From: jacob.kabel-at-ubc.ca
Date: Wed, 31 Aug 2016 18:40:52 -0500
Subject: [Microscopy] Re: viaWWW:Strange Pattern on SEM Images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Arezoo,

You are seeing residual charging from the beam as it is drawn during the
auto brightness and contrast routine.

When you press the auto brightness and contrast button, your SEM likely
does the following:

1) Draw a figure 8 and sample the signal from it within the field of view
2) Adjust contrast and brightness
3) Repeat from step 1 until acceptable contrast and brightness is
achieved (output waveform min/max within parameters)

This allows the SEM to get a representative sample of the entire imaging
area at an acceptable per pixel dwell time without having to collect a
whole image.

-Jacob


On 16-08-31 04:26 PM, microscopy.listserver-at-gmail.com wrote:
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
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}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at http://www.microscopy.com/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when replying please copy both
} arezoo.zare-at-okstate.edu as well as the Microscopy Listserver
} ---------------------------------------------------------------------------
}
} Email: arezoo.zare-at-okstate.edu Name: Arezoo Zare
}
} Organization: Oklahoma State University
}
} Title-Subject: [Filtered] Strange Pattern on SEM Images
}
} Message: Hello everyone,
}
} I have a question about a strange pattern that shows up on some of my SEM images. I have a SiOC
} specimen which does not have a good conductivity but since I need the specimen for other tests I do
} not want to coat it. As a result the specimen charges up under SEM and sometimes I can clearly see
} the zoom window being brighter than other areas. Until now everything seem reasonable but as soon as
} I click on auto brightness/contrast button, a strange pattern appears on the image. The observed
} pattern is similar to a few intermittent infinity symbols or something that you draw with a
} harmonograph. The pattern only shows up on this sample and not on the other ones. I was wondering if
} anyone has any idea about the origin of the observed pattern. I was not able to attach the image
} here but I am whiling to share it via e-mail. Thank you for your attention.
}
} My best,
} Arezoo
}
} Login Host: 139.78.252.128
} Listserver Email Form V - 20120416
} ---------------------------------------------------------------------------
}
}
}

--
Jacob Kabel
Electron Microscopist
Department of Materials Engineering
The University of British Columbia
6350 Stores Road, Room 419
Vancouver, British Columbia
Canada
V6T 1Z4
Phone: (604) 822-5648
Fax: (604) 822-3619
http://emlab.mtrl.ubc.ca/


==============================Original Headers==============================
10, 33 -- From jacob.kabel-at-ubc.ca Wed Aug 31 18:40:52 2016
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10, 33 -- Subject: Re: [Microscopy] viaWWW:Strange Pattern on SEM Images
10, 33 -- To: {arezoo.zare-at-okstate.edu} , {Microscopy-at-microscopy.com}
10, 33 -- References: {201608312326.u7VNQMvT008725-at-ns.microscopy.com}
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From: microscopy.listserver-at-gmail.com
Date: Wed, 31 Aug 2016 20:04:46 -0500
Subject: [Microscopy] viaWWW:Strange Pattern on SEM Images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html






X-from: jacob.kabel-at-ubc.ca
Reply-To: jacob.kabel-at-ubc.ca
To: microscopy.listserver-at-gmail.com




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Hi Arezoo,

You are seeing residual charging from the beam as it is drawn during the
auto brightness and contrast routine.

When you press the auto brightness and contrast button, your SEM likely
does the following:

1) Draw a figure 8 and sample the signal from it within the field of view
2) Adjust contrast and brightness
3) Repeat from step 1 until acceptable contrast and brightness is
achieved (output waveform min/max within parameters)

This allows the SEM to get a representative sample of the entire imaging
area at an acceptable per pixel dwell time without having to collect a
whole image.

-Jacob


On 16-08-31 04:26 PM, microscopy.listserver-at-gmail.com wrote:
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} X-from: arezoo.zare-at-okstate.edu
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at http://www.microscopy.com/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when replying please copy both
} arezoo.zare-at-okstate.edu as well as the Microscopy Listserver
} ---------------------------------------------------------------------------
}
} Email: arezoo.zare-at-okstate.edu Name: Arezoo Zare
}
} Organization: Oklahoma State University
}
} Title-Subject: [Filtered] Strange Pattern on SEM Images
}
} Message: Hello everyone,
}
} I have a question about a strange pattern that shows up on some of my SEM images. I have a SiOC
} specimen which does not have a good conductivity but since I need the specimen for other tests I do
} not want to coat it. As a result the specimen charges up under SEM and sometimes I can clearly see
} the zoom window being brighter than other areas. Until now everything seem reasonable but as soon as
} I click on auto brightness/contrast button, a strange pattern appears on the image. The observed
} pattern is similar to a few intermittent infinity symbols or something that you draw with a
} harmonograph. The pattern only shows up on this sample and not on the other ones. I was wondering if
} anyone has any idea about the origin of the observed pattern. I was not able to attach the image
} here but I am whiling to share it via e-mail. Thank you for your attention.
}
} My best,
} Arezoo
}
} Login Host: 139.78.252.128
} Listserver Email Form V - 20120416
} ---------------------------------------------------------------------------
}
}
}

--
Jacob Kabel
Electron Microscopist
Department of Materials Engineering
The University of British Columbia
6350 Stores Road, Room 419
Vancouver, British Columbia
Canada
V6T 1Z4
Phone: (604) 822-5648
Fax: (604) 822-3619
http://emlab.mtrl.ubc.ca/


==============================Original Headers==============================
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10, 33 -- Subject: Re: [Microscopy] viaWWW:Strange Pattern on SEM Images
10, 33 -- To: {arezoo.zare-at-okstate.edu} , {Microscopy-at-microscopy.com}
10, 33 -- References: {201608312326.u7VNQMvT008725-at-ns.microscopy.com}
10, 33 -- From: Jacob Kabel {jacob.kabel-at-ubc.ca}
10, 33 -- Message-ID: {0c77a3b7-5feb-ab5d-b792-29165720cfb3-at-ubc.ca}
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From: microscopy.listserver-at-gmail.com
Date: Wed, 31 Aug 2016 15:30:33 +0000
Subject: Re: [Microscopy] viaWWW:Dehydration/infiltration issues

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Email: buchsmith-at-gmail.com Name: JoAnn Buchanan

Organization: Allen Institute for Brain Science

Title-Subject: [Filtered] Dehydration/infiltration issues

Message: We recently started using a processor for dehydration and initial infiltration steps. We
are processing very large samples-1 to 1.5 mm.
Since trying the processor, we have gotten poorly infiltrated samples, mainly in the center of the
block. This happened even after we doubled the dehydration times.
We use ethanol series followed by acetonitrile as an intermediate and Hard Plus resin for embedding.
We only do a 2p solvent to 1 Part resin in the processor. We don't have this problem with bench
processing.

Since our protocol is 9 days long, a processor for dehydration really helps. I'd really like to be
able to use the processor for this. and infiltration.

Any troubleshooting ideas or suggestions are welcome.
Thanks in advance,

JoAnn

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From: microscopy.listserver-at-gmail.com
Date: Thu, 1 Sep 2016 06:52:35 -0500
Subject: [Microscopy] viaWWW:SSOM 3D Symposium 2016

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Email: marco.cantoni-at-epfl.ch Name: Marco Cantoni

Organization: EPFL-CIME

Title-Subject: [Filtered] SSOM 3D Symposium 2016

Message: Abstract and registration deadline is approaching!
Check the updated program.
Dont miss this event.
SSOM Interdisciplinary Symposium on 3D Microscopy 2016 in Les Diablerets.
http://cime.epfl.ch/3D-Symposium2016

Best regards

Marco Cantoni

______________________________
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From: microscopy.listserver-at-gmail.com
Date: Sat, 3 Sep 2016 08:20:53 -0500
Subject: [Microscopy] viaWWW:X-Ray Shielding in SEM

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Email: studentstef-at-googlemail.com Name: Stefan

Organization: University

Title-Subject: [Filtered] X-Ray Shielding in SEM

Message: Hallo everyone,

I have a question about X-Ray shielding in a scanning electron microscope and I hope you can help
me! The topic is that when electrons hit the specimen, characteristic radiation up to 10keV is
generated. This radiation is blocked/decelerated my the surrounding tower and chamber that are
shielded with mu-metal (how thick is this coating). If I would just use pure Aluminium the X-Ray
shielding wouldn't be enough. So far, is this correct?
Because Aluminium has a half-value thickness of about 112mm for 10keV x-radiation. I am a bit
concerned that some new-built parts of our SEM-chamber-cover aren't enough to shield the generated
x-radiation. They are made out of alluminium with a thin mu-metal coating. It may be enough for
magnetic field but I don't know if it is enough for the x-rays.. Can anyone tell me if my concerns
are legitimate or is the X-radiation easily shielded with such layers?

Thank you for your help!



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From: microscopy.listserver-at-gmail.com
Date: Sat, 3 Sep 2016 08:21:31 -0500
Subject: [Microscopy] viaWWW: Thank you for responses infiltration/dehydration issues

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Email: buchsmith-at-gmail.com Name: JoAnn Buchanan

Organization: Allen Institute for Brain Science

Title-Subject: [Filtered] Thank you for responses infiltration/dehydration issues

Message: Dear listers. i appreciate all the responses I got to my question. After I posted, I took
a closer look at the instrument and saw that the caps were not aligned properly with the vials,
causing a gap when the lid was lowered.

I sent an image to the company and it was quickly ascertained that it is likely a defect in the
equipment. Hopefully, our problems will be solved by having the lid repaired or the unit replaced.
JoAnn


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From: microscopy.listserver-at-gmail.com
Date: Sat, 3 Sep 2016 08:22:43 -0500
Subject: [Microscopy] viaWWW:Call for abstracts: 2017 Quantitative BioImaging Conference

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Email: sripad.ram-at-gmail.com Name: Sripad Ram

Organization: Quantitative BioImaging Conference

Title-Subject: [Filtered] Call for abstracts: 2017 Quantitative BioImaging Conference

Message: Can you please post the following conference announcement in the listerv?

Dear colleague,
After a successful meeting at Delft, The Netherlands in 2016, we are pleased to announce that the
5th international quantitative bioimaging conference will be held on Jan 5- 7, 2017 at Texas A&M
University, College Station, TX. The upcoming conference will continue to focus on the quantitative
analysis of bioimaging data in an interdisciplinary manner.
Abstract submission: We seek contributions in any area of quantitative microscopy. Presentations
that demonstrate in detail new approaches are particularly welcome, including but not limited to
algorithmic and software developments, physical modeling approaches etc. The use of quantitative
imaging techniques in biological applications is also of great interest. For submission details
please see the conference website (www.QuantitativeBioImaging.com).

ABSTRACTS ARE DUE BY SEPTEMBER 15, 2016

Key note speakers:
• Ernst Stelzer, Buchmann Institute for Molecular Life Sciences, Frankfurt, Germany
• Marino Zerial, Max Planck Institute Dresden, Germany
• Robert Singer, Albert Einstein College of Medicine, New York, USA

Invited Speakers (confirmed): C. Calderon (Denver, USA); C. Cogswell (Boulder, USA); S. Cox (London,
UK); Y. Kalaidzidis (Dresden, Germany); P. Kner (Athens, Georgia, USA); U. Kubitscheck (Bonn,
Germany); E. Lemke (Heidelberg, Germany); K. Lidke (Albuquerque, NM, USA); J. Liphardt (Stanford,
USA); J-C. Olivo-Marin (Paris, France); B. Rieger (Delft, The Netherlands); J. Rittscher (Oxford,
UK); N. Stuurman (San Francisco, USA); G. Rohde (Charlottesville, VA, USA); K. Rohr (Heidelberg,
Germany); Y. Shechtman (Stanford, USA); S. Stallinga (Delft, The Netherlands); N. Thompson (Chapel
Hill, HC, USA); W. Wiegraebe (Seattle, USA).

Registration fees: In line with prior years, there will be no registration fees for academic attendees.
We look forward to seeing you in College Station in January.
Sincerely,
The organizers of QBI2017



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From: larry.stoter-at-gmail.com
Date: Sat, 3 Sep 2016 09:10:15 -0500
Subject: [Microscopy] Re: viaWWW:X-Ray Shielding in SEM

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X-rays up to the primary electron beam energy are generated inside the SEM - if that is 30 kev, then some of the X-rays generated will be at 30 keV. If it’s 10 keV, then X-rays to 10 keV will be generated.

Mu-metal is for stopping stray external electromagnetic fields influencing the SEM incident electron beam - it has nothing directly to do with X-ray shielding, although it will obviously absorb some X-rays but that isn’t its primary purpose. For optimum shielding, the mu-metal needs to be on the outside of the chamber/column in which position it really isn’t going to be of any consequence for X-ray shielding - if the X-rays have got that far, you have a real problem.

Aluminium is used to stop electrons - 30 keV electrons hitting Al generate Al X-rays, predominatly at around 1.48 keV, which are easily absorbed. 30 keV electrons hitting steel are going to generate mainly Fe X-rays at around 7 kev - not so easy to absorb. Hitting lead, they generate Pb L X-rays at up to 12 keV, more difficult still to absorb.

I would suggest that an Al-only port cover for an SEM is not acceptable from a radiation safety viewpoint. Have a look at the other port covers on the SEM chamber and replicate them in design, materials and thickness and even if you are only using the SEM at up to 10 keV, consider that it is probably capable of 30 keV? A straightforward 15 mm thick steel plate is probably going to do the job. For something more sophisticated, 2 mm of Al on the inside and some mu-metal on the outside.

Larry Stoter

} On 3 Sep 2016, at 14:42, microscopy.listserver-at-gmail.com wrote:
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} Email: studentstef-at-googlemail.com Name: Stefan
}
} Organization: University
}
} Title-Subject: [Filtered] X-Ray Shielding in SEM
}
} Message: Hallo everyone,
}
} I have a question about X-Ray shielding in a scanning electron microscope and I hope you can help
} me! The topic is that when electrons hit the specimen, characteristic radiation up to 10keV is
} generated. This radiation is blocked/decelerated my the surrounding tower and chamber that are
} shielded with mu-metal (how thick is this coating). If I would just use pure Aluminium the X-Ray
} shielding wouldn't be enough. So far, is this correct?
} Because Aluminium has a half-value thickness of about 112mm for 10keV x-radiation. I am a bit
} concerned that some new-built parts of our SEM-chamber-cover aren't enough to shield the generated
} x-radiation. They are made out of alluminium with a thin mu-metal coating. It may be enough for
} magnetic field but I don't know if it is enough for the x-rays.. Can anyone tell me if my concerns
} are legitimate or is the X-radiation easily shielded with such layers?
}
} Thank you for your help!



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From: lists-at-nexperion.net
Date: Sat, 3 Sep 2016 09:39:15 -0500
Subject: [Microscopy] EM Core Facility Position in Gothenburg, Sweden

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues,

I was asked to spread the word about an EM Researcher position at the National Centre for Cellular Imaging at the University of Gothenburg, Sweden. Details about the institution, the job profile, and the required qualification can be found at:

http://www.gu.se/english/about_the_university/announcements-in-the-job-application-portal/?languageId=0&disableRedirect=true&id=19144&Dnr=782834&Type=E

Best,

Guenter

--
Dr. Guenter Resch
Nexperion e.U. - Solutions for Electron Microscopy - www.nexperion.net
Mattiellistrasse 3/17, 1040 Vienna, Austria - Phone +43 664 94 17 210
Registered at Commercial Court Vienna, FN 397677w - VAT Reg. ATU67962234

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From: brachfelds-at-mail.montclair.edu
Date: Sun, 4 Sep 2016 13:24:56 -0500
Subject: [Microscopy] Tenure Track Position in Sustainability Science at Montclair State

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http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues,
This is slightly off-topic for this microscopy list, but I would appreciate your help in sharing this job announcement with your faculty, post-docs, and graduate students who might be interested.
Thank you,
Stefanie

FACULTY POSITION IN SUSTAINABILITY SCIENCE
The Department of Earth and Environmental Studies invites applications for a full-time (10-month) tenure-track Assistant Professor position in Sustainability Science. Applicants with expertise in areas including but not limited to urban sustainability, urban design and regional planning, climate change mitigation and adaptation, industrial ecology, renewable energy, carbon auditing, and life cycle assessment, are encouraged to apply. The successful candidate will develop a vigorous externally funded research program and have a strong commitment to excellence in teaching. The candidate will be expected to play a critical role in our bachelors, masters, and professional science masters programs in Sustainability Science, and contribute to teaching and mentoring in our Geography, Earth and Environmental Sciences, and Environmental Management bachelors, masters, and doctoral programs. Teaching responsibilities will include introductory courses as well as upper-level undergraduate and graduate courses within the applicants areas of expertise. Service to the department, university, and larger professional community is also expected.

QUALIFICATIONS:Applicants are expected to have a Ph.D. in sustainability science, environmental science, urban planning, geosciences, geography, resource management, or other appropriate field, a record of peer-reviewed scholarship in sustainability, and evidence of potential for success in grant activity. Post-doctoral experience is desirable.

SALARY RANGE: Salary and range is dependent upon qualifications
STARTING DATE: September 1, 2017

APPLICATIONS:Electronic applications assembled into a single combined .pdf or .doc file and containing a cover letter, CV, statements of teaching and research interests, and the names and contact information for three professional references should be sent to EAESsearch-at-mail.montclair.edu. Applications are due byOctober 15, 2016.

*********************
Dr. Stefanie A. Brachfeld
Professor and Chair,Department of Earth & Environmental Studies
Director, PhD Program in Environmental Management
Montclair State University
Montclair, NJ 07043
phone: (973) 655-5129
fax: 973-655-4072
brachfelds-at-mail.montclair.edu
*********************


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From: tbargar-at-unmc.edu
Date: Tue, 6 Sep 2016 15:31:06 -0500
Subject: [Microscopy] a H & E protocol for 1 micron thick araldite sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,

Does anyone out there have a good protocol for staining 1 micron thick resin sections (in my case it is Araldite), using Hematoxylin and Eosin?

Tom Bargar
UNMC
Electron Microscopy Core Facility
986395 Nebraska Medical Center
Omaha, NE 68198-6395
402-559-7347
tbargar-at-unmc.edu


The information in this e-mail may be privileged and confidential, intended only for the use of the addressee(s) above. Any unauthorized use or disclosure of this information is prohibited. If you have received this e-mail by mistake, please delete it and immediately contact the sender.



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From: wij.muss-at-aon.at
Date: Wed, 7 Sep 2016 04:46:25 -0500
Subject: [Microscopy] Re: a H & E protocol for 1 micron thick araldite sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Tom,

just as a quick shot....therefore hopefully as short as acceptable in
Listserver communication:

as to my knowledge H&E on (hydrophobic) Araldite (or also Epon/epoxy
semithin) resin sections can be done only after harsh chemical treatment
(like either etching, or - sometimes also removal of resin)...i. e. use of
oxidants (like H2O2, or epoxy "solvents" like sodium (or
potassium-)methylate

Google.com Search for: {Araldite AND resin AND section AND staining AND H&E
OR hematoxylin OR haematoxylin AND eosin} reveals many references....
Google.com Search for: {Araldite AND resin AND section AND staining AND H&E
OR hematoxylin OR haematoxylin AND eosin AND ETCHING} approx. 925 results
Search https://scholar.google.com: for: {Araldite AND resin AND section AND
staining AND H&E OR hematoxylin OR haematoxylin AND eosin} approx. 2950
results
Search https://scholar.google.com: for: { Araldite AND resin AND section AND
staining AND H&E OR hematoxylin OR haematoxylin AND eosin AND ETCHING}
approx.117 results


Perhaps as a {standard} recipe (which might not be very successful for
several reasons...:
Application of standard micro-anatomical staining methods to epoxy
resin-embedded sections
S. R. Aparicio and P. Marsden ( PMCID: PMC474254)
J Clin Pathol. 1969 Sep; 22(5): 589592.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC474254/
or pdf:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC474254/pdf/jclinpath00382-0079.p
df

and/or
WARMKE H.E. & LEE S-L.J. : Improved staining procedures for semithin epoxy
sections of plant tissues
Stain Technol 51 No3, 179-185, 1976

Knowing that there has been described (by references) H&E-staining applied
to resin sections which on serious insight turned out to be wrong
descriptions or false supposition (e. g. naming of procedure)...
Ending for now - but not at the End of knowledge...
Best wishes-good luck- regards,

Wolfgang Muss PhD
Retired, Member of MSA
SALZBURG, AUSTRIA

============================================================================
===================




-----Ursprngliche Nachricht-----
Von: tbargar-at-unmc.edu [mailto:tbargar-at-unmc.edu]
Gesendet: Dienstag, 6. September 2016 23:04
An: wij.muss-at-aon.at
Betreff: [Microscopy] a H & E protocol for 1 micron thick araldite sections

----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Dear Listers,

Does anyone out there have a good protocol for staining 1 micron thick resin
sections (in my case it is Araldite), using Hematoxylin and Eosin?

Tom Bargar
UNMC
Electron Microscopy Core Facility
986395 Nebraska Medical Center
Omaha, NE 68198-6395
402-559-7347
tbargar-at-unmc.edu


The information in this e-mail may be privileged and confidential, intended
only for the use of the addressee(s) above. Any unauthorized use or
disclosure of this information is prohibited. If you have received this
e-mail by mistake, please delete it and immediately contact the sender.



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From: hsia627-at-hotmail.com
Date: Wed, 7 Sep 2016 10:47:48 -0500
Subject: [Microscopy] Cryo-ultramicrotomy course announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues,

We are pleased to announce that the Electron Microscopy Core Imaging Facility (EMCIF) at the University of Maryland Baltimore will be offering a cryo-ultramicrotomy mini-course on October 17th and 18th, 2016. The course is designed to teach advanced users of cryo-ultramicrotomy techniques. The format includes lectures, demonstrations and hands on practice. Participants must be familiar with room temperature ultramicrotome operation and techniques. Registration will be limited to a maximum of six participants. More information regarding to the course and registration can be found in our website

http://www.dental.umaryland.edu/2016cryoultramicrotomycourse/

A companion Immuno Gold Silver Staining (IGSS) workshop sponsored by EMS-Diatome and Aurion will be held at EMCIF on October 19th to 21st after the cryo ultramicrotomy minicourse. New to this years program, the workshop will also include Tokuyasu immunolabeling method. Individuals who are interested to learn Tokuyasu immunogold labeling techniques are strongly encouraged to participate both workshops. A $50 discount will be offered for registration of both courses.

For more information about the IGSS workshop, visit:

http://www.emsdiasum.com/microscopy/products/immunogold/workshop.aspx

Please email coreimaging-at-umaryland.edu or visit our website
http://www.dental.umaryland.edu/core-imaging/workshops-and-courses/ for more information.

Thanks.

Ru-ching


Ru-ching Hsia, Ph.D.
rhsia-at-umaryland.edu
Associate Professor and Director
Electron Microscopy Core Imaging Facility
University of Maryland, Baltimore
Tel: 410-706 7992
http://www.dental.umaryland.edu/Core-imaging
Rm 696B, Howard Hall, 660 W. Redwood St. Baltimore, MD 21201

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From: steven.spurgeon-at-pnnl.gov
Date: Wed, 7 Sep 2016 11:40:37 -0500
Subject: [Microscopy] Importing Noran System 7 EDS SI into Digital Micrograph

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi everyone,

Does anyone know of a good way to export EDS spectrum images from the Noran System 7 software and then import them into Digital Micrograph? I’ve found a way to export the EDS data in raw format, but I don’t know how to load it into DM properly.

Any thoughts? Thanks!

______________________________________
Steven R. Spurgeon, Ph.D.
Postdoctoral Research Associate
Physical and Computational Sciences Directorate

Pacific Northwest National Laboratory
902 Battelle Boulevard
P.O. Box 999 MSIN:K8-87
Richland, WA 99352

Tel: +1-509-371-7123
steven.spurgeon-at-pnnl.gov
www.stevenspurgeon.com {http://www.stevenspurgeon.com/}
www.pnnl.gov {http://www.pnnl.gov/}



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8, 26 -- Subject: Importing Noran System 7 EDS SI into Digital Micrograph
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From: dev.c0debabe-at-gmail.com
Date: Wed, 7 Sep 2016 12:07:25 -0500
Subject: [Microscopy] Staining of Cu for SEM imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

we are using a older model SEM to image samples with copper structures
on the surface and just a few nm below the surface at the same time
(typically buried under a thin layer of glass).
Since the copper layer below the surface is just covered by this very
thin glass layer, even with lower acceleration voltages it is very hard
to distinguish on the images whether the imaged copper structures are
located on the surface or below it.
As our goal would be that the copper on the surface clearly looks
different from the copper buried under the thin glass layer, we were
wondering whether we could use a staining procedure.

For instance, we are aware of a wet chemical procedure allowing to
tin-plate copper. However, without trying it we don't know if it would
result in a considerable different contrast in the SEM as the atomic
mass of Sn is very close to the one of Cu.
Wet chemical gold plating on the other hand involves not only expensive
but also highly toxic chemicals.

Are you aware of (wet chemical) staining procedures for copper that
produce a considerable contrast and are preferably inexpensive as well
as non highly toxic ?

Is there maybe another technique we could use ?

Kind regards,
Stefan

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From: steven.spurgeon-at-pnnl.gov
Date: Thu, 8 Sep 2016 18:51:25 -0500
Subject: [Microscopy] Importing Noran System 7 EDS SI into Digital Micrograph

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi everyone,

Does anyone know of a good way to export EDS spectrum images from the Noran System 7 software and then import them into Digital Micrograph? I’ve found a way to export the EDS data in raw format, but I don’t know how to load it into DM properly.

Any thoughts? Thanks!

______________________________________
Steven R. Spurgeon, Ph.D.
Postdoctoral Research Associate
Physical and Computational Sciences Directorate

Pacific Northwest National Laboratory
902 Battelle Boulevard
P.O. Box 999 MSIN:K8-87
Richland, WA 99352

Tel: +1-509-371-7123
steven.spurgeon-at-pnnl.gov
www.stevenspurgeon.com {http://www.stevenspurgeon.com/}
www.pnnl.gov {http://www.pnnl.gov/}



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8, 26 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
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From: microscopy.listserver-at-gmail.com
Date: Thu, 8 Sep 2016 19:08:38 -0500
Subject: [Microscopy] viaWWW:Fungi

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Title-Subject: [Filtered] Fungi

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From: microscopy.listserver-at-gmail.com
Date: Thu, 8 Sep 2016 19:09:42 -0500
Subject: [Microscopy] via WWW: Immunogold spatial resolution

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Email: n.i.clarke-at-warwick.ac.uk Name: Nick Clarke

Organization: University of Warwick

Title-Subject: [Filtered] Immunogold spatial resolution.

Message: Hello, I was wondering if you could provide me with a good reference for immunogold spatial
resolution i.e. distance from antigen to centre of gold particle. Any help would be really
appreciated with this. Kind regards, Nick.

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From: microscopy.listserver-at-gmail.com
Date: Thu, 8 Sep 2016 19:11:09 -0500
Subject: [Microscopy] viaWWW:Hitachi S-2700 SEM user manuals

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Email: brad-at-quant-corp.com Name: Brad Lindner

Organization: Quant Corporation

Title-Subject: [Filtered] Hitachi S-2700 SEM

Message: I recently purchased a used Hitachi S-2700 SEM from a local university but do not have any
of the user guides / manuals. Does anyone have these that would be willing to send in PDF form?

Additionally, I would like to verify that I've set up all of the components correctly before I turn
the system on - are there any independent SEM/EDS service companies in the Milwaukee, WI area that I
could hire for this and regular maintenance?

Thanks for your time,
Brad

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From: microscopy.listserver-at-gmail.com
Date: Thu, 8 Sep 2016 19:11:54 -0500
Subject: [Microscopy] viaWWW:TEM of mouse neuromuscular junction

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Email: marie.cantino-at-gmail.com Name: Marie Cantino

Organization: University of Connecticut

Title-Subject: [Filtered] TEM of mouse neuromuscular junction

Message: I have been asked to help a student who would like to do TEM of mouse neuromuscular
junctions (NMJ). I’ve done plenty of TEM on muscle, but never looking specifically for NMJ. This
strikes me as a bit of a needle in a haystack type of effort, since there is generally only one, or
possibly a few per fiber, depending on the fiber type. Does anyone have experience with this? I
see some studies use cholinesterase staining to find the NMJ first at the light level. Is this
technique tricky? Are there other tricks i should know about? Will this require hundreds of
hours of microtomy? Thanks for any help or advice you can offer.


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From: dev.c0debabe-at-gmail.com
Date: Thu, 8 Sep 2016 19:26:46 -0500
Subject: [Microscopy] Staining of Cu for SEM imaging

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{ { { No Message Collected } } }

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1, 20 -- From: Stefan Schoenleitner {dev.c0debabe-at-gmail.com}
1, 20 -- Subject: Staining of Cu for SEM imaging
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From: kraftpiano-at-gmail.com
Date: Thu, 8 Sep 2016 19:34:26 -0500
Subject: [Microscopy] Moran stage schematic

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Does anybody have schematics or a service guide available for an older Noran ULGS-48 stage? I really just need the pinouts for the connections to the motors. I would like to replace the dying electronics with something a little more modern. It’s a 5-axis replacement stage in a JEOL 848.

Thank you in advance!

—Justin A. Kraft

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From: microscopy.listserver-at-gmail.com
Date: Thu, 8 Sep 2016 19:43:26 -0500
Subject: [Microscopy] viaWWW:

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X-from: lucy.avati-at-sydney.edu.au

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Email: lucy.avati-at-sydney.edu.au Name: Lucy Avati

Organization: University of Sydney

Title-Subject: [Filtered] Job opportunity: Microscopy Officer at the University of Sydney

Message: Microscopy Officer
School of Medical Sciences - Bosch Institute
Sydney Medical School
Reference No. 1519/0916


• Located at Camperdown Campus • Part-time 0.5 FTE, fixed term for 12 months • Remuneration package:
$95K-$104K p.a. pro rata which includes leave loading and up to 17% super


The University of Sydney is Australia's first university and has an outstanding global reputation
for academic and research excellence. It employs over 7600 permanent staff, supporting over 60,000
students.

The Bosch Institute is the research arm of the School of Medical Sciences, Sydney Medical School.
Its role is to add value to the research activities of the SMS through the provision of well-managed
core facilities that include new and emerging technologies and provide excellent training for junior
scientists.

We are seeking a Microscopy Officer to provide direct support to the Advanced Microscopy Facility
(AMF) Core Facilities Manager at the Bosch Institute.

You will:
• be a point of contact for the ZEISS AxioScan high-throughput digital slide-scanning microscope and
provide a slide-scanning service for the Institute
• train students and researchers on the operation of the transmitted light, fluorescence and
confocal microscopes in the Facility through individual and group teaching sessions
• carry out administrative duties including ordering, processing AMF registration and access and
submission of grant applications.

You will need:
• PhD or equivalent – “soon to be awarded” or “thesis submitted” may be acceptable, depending on
other knowledge, skills, experience
• demonstrated experience having worked in a biomedical research environment
• experience with light, fluorescence and confocal microscopy
• experience with general administrative duties
• experience in teaching/demonstrating at tertiary level
• experience with publication of scientific papers in peer-reviewed journals
• knowledge of basic and advanced microscope methods
• experience with the operation of basic and advanced fluorescence microscopes
• ability to interact positively with members of the university community, including academic staff,
research students, general staff
• understanding of computer operation, including software applications.

Highly desirable:
• experience with digital slide slide-scanning microscopy
• experience with laser microdissection and/or optical tweezers
• experience with multi-photon microscopy
• experience with preparation of applications to funding bodies
• experience with University of Sydney structure, environment, operations
• knowledge of image analysis and processing software

All applications must be submitted via the University of Sydney careers website. Visit
sydney.edu.au/recruitment and search by the reference number for more information and to apply.

CLOSING DATE: 5pm 23 September 2016

The University is an equal opportunity employer committed to equity, diversity and social inclusion.
Applications from equity target groups, including women and people with disabilities are encouraged.
As the University of Sydney has established a scheme to increase the number of Aboriginal and Torres
Strait Islander staff employed across the institution, applications from people of Aboriginal and
Torres Strait Islander descent are also encouraged.
© The University of Sydney

The University reserves the right not to proceed with any appointment.


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From: wim.hagen-at-me.com
Date: Thu, 8 Sep 2016 21:31:29 -0500
Subject: [Microscopy] Wind turbines & TEM systems

Contents Retrieved from Microscopy Listserver Archives
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Dear all,

There are plans for several very large wind turbines close to our Tecnai and Titan TEM systems. There are reports about low frequency (1-10 Hz) ground vibrations generated by wind turbines. The typical passive dampening systems on TEM systems have a resonance frequency of around 7 Hz and then dampening starts.

I would be interested to hear about any experiences with wind turbines close to (~800 meters) high-end TEM systems.

Thanks in advance,

Wim

==============================Original Headers==============================
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From: hsia627-at-hotmail.com
Date: Thu, 8 Sep 2016 22:00:11 -0500
Subject: [Microscopy] Cryo-ultramicrotomy course announcement

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{ { { No Message Collected } } }

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From: leunissen-at-aurion.nl
Date: Thu, 8 Sep 2016 22:00:06 -0500
Subject: [Microscopy] Re: via WWW: Immunogold spatial resolution

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Good morning,

Prof Rob Parton from Brisbane is one of the people who looked into this. I have no papers at hand at the moment, but I am sure a search will easily reveal his work in this area,

Good luck

Jan Leunissen
Aurion



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} Title-Subject: [Filtered] Immunogold spatial resolution.
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} Message: Hello, I was wondering if you could provide me with a good reference for immunogold spatial
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From: wij.muss-at-aon.at
Date: Thu, 8 Sep 2016 22:48:50 -0500
Subject: [Microscopy] Re: a H & E protocol for 1 micron thick araldite sections

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From: peter.heimann-at-uni-bielefeld.de
Date: Fri, 9 Sep 2016 08:22:43 -0500
Subject: [Microscopy] Re: TEM of mouse neuromuscular junction ( Peter Heimann )

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Hello Marie,

I investigated the fine structure of NMJ's in the mouse and would propose:
perfuse the animal by standard procedure and dissect _before
_osmification step. Try either:

(a) m. sternocostalis (transversus thoracis muscle) on the inner side of
the rib cage, not easy to dissect since only 2-4 layers of muscle fibers
thick and extremely delicate, however all nmj's are positioned in one
"line" and once you know where to look, you have a high number of NMJ's
in one section. Pin down the flattened muscle in a Sylgard dish with
fine insect needles and osmicate. Flat-embed the specimen and cut out
target region as a rectangular strip under binocular and polymerize on
an empty resin block.

(b) dissect the thin strip of intercostal muscles ("rib muscles")
between two ribs, NMJ's are typically located in the "mid-line" between
the two ribs

(c) dissect the muscle part of the diaphragm ( in this case don't hurt
the diaphragm during perfusion), wrap it up into a "roll", most nmj's
are more or less in the mid of the length of the muscle fibers, and osmicate

(d) if a-c fails, dissect the tongue, osmicate, embed, cut and look in
the EM for nerves resp axons (you can spot them beforehand on a semithin
section). if you see a small nerve or individual (myelinated) axons,
then usually a NMJ is not far away, it takes however some time to search
....

- in general: on a good Richadson-blue-stained semi-thin section you can
identify the NMJ's easily with a x63 objective
- always first check on a parallel specimen location of NMJ's by
acetylcholine-esterase (ACHE) staining, takes 20 minutes at most (ACHE
works good on formaldehyde fixed tissue too). ACHE staining is robust,
stable and uncritical and MUST work, just take care that you store the
acetylthiocholinejodide dry and at -20 Celsius ....

good luck !

Peter

+++++++++++++++++++++++++++++++++


Email: marie.cantino-at-gmail.com Name: Marie Cantino
} Organization: University of Connecticut
}
} Title-Subject: [Filtered] TEM of mouse neuromuscular junction
}
} Message: I have been asked to help a student who would like to do TEM of mouse neuromuscular
} junctions (NMJ). I’ve done plenty of TEM on muscle, but never looking specifically for NMJ. This
} strikes me as a bit of a needle in a haystack type of effort, since there is generally only one, or
} possibly a few per fiber, depending on the fiber type. Does anyone have experience with this? I
} see some studies use cholinesterase staining to find the NMJ first at the light level. Is this
} technique tricky? Are there other tricks i should know about? Will this require hundreds of
} hours of microtomy? Thanks for any help or advice you can offer.
}
}
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--
====================================================

Dr. Peter Heimann Raum: W7-107 / Tel.: +49-(0)521-106-5628
Universitaet Bielefeld Fax: +49-(0)521-106-5654
"Zellbiologie / Cell Biology" W7-107
33501 Bielefeld, Germanywww.uni-bielefeld.de/biologie/cellbio


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From: microscopy.listserver-at-gmail.com
Date: Fri, 9 Sep 2016 08:44:02 -0500
Subject: [Microscopy] Administrivia: Mail server headaches

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Colleagues

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From: vray-at-partbeamsystech.com
Date: Sun, 11 Sep 2016 00:45:15 -0500
Subject: [Microscopy] Re: Staining of Cu for SEM imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Stefan,

There are few things you can try to distinguish between exposed and
slightly-buried Cu conductors:

1. Based on your mentioning of "atomic mass of Sn is very close to the
one of Cu" I am suspecting you may be using backscattered electrons for
imaging - try secondary electron detector imaging at lowest
acceleration you can afford without loosing too much resolution.

2. Apply negative bias to the sample (retarding field) to reduce landing
energy of primary electrons. This could work or not, depending on
geometry of your instrument, stage, and your instrumentation
skills/capabilities.

3. Electroless nickel plating chemicals cost less then Au plating kits,
though either one is safe enough to do in ordinary fume hood with gloves
and common sense.

4. Exposure to iodine vapor in humid atmosphere (room air) would corrode
exposed Cu fairly quickly, making it look "fluffy" or "rough," while
buried conductors will remain intact for a while. The tricky part would
be to image/scan the sample fairly quickly - once started the corrosion
would eventually consume all the Cu layers. You want to image exposed
layer once it has been just so slightly corroded on the surface, and
immediately polish it away to prevent damage for the underlying layers.

5. Either sulfuric or nitric acid with H2O2 should etch Cu
crystallographically, making exposed surface look differently then metal
protected by dielectric. You would want to experiment with "disposable"
devices and develop reliable procedure prior to immersing "real" one.
Same is true for plating, by the way.

6. Image the chip, etch away Cu with FeCl3, wash-rinse, and the image
same area again. Buried under dielectric Cu would be on both images,
while exposed would disappear after etching. You would need to develop
procedure and control time/temperature/concentration of etchant to get
repeatable results.

7. Do forward-scattered imaging, or even regular Se imaging at glancing
angle - it would be much more sensitive to presence of thin dielectric
layer. Foreshortening correction would be required....

Happy Imaging :)

Valery


Valery Ray
www.linkedin.com/in/valeryray
==============================
PBS&T, MEO Engineering Company
290 Broadway, Suite 298
Methuen, MA 01844, USA
Phone: +1-978-305-0479 - leave a message
Mobie: +1-978-305-0479 - leave a message
E-mail: vray-at-partbeamsystech.com
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Web: www.freudlabs.com

On 9/7/2016 2:24 PM, dev.c0debabe-at-gmail.com wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
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} Hi,
}
} we are using a older model SEM to image samples with copper structures
} on the surface and just a few nm below the surface at the same time
} (typically buried under a thin layer of glass).
} Since the copper layer below the surface is just covered by this very
} thin glass layer, even with lower acceleration voltages it is very hard
} to distinguish on the images whether the imaged copper structures are
} located on the surface or below it.
} As our goal would be that the copper on the surface clearly looks
} different from the copper buried under the thin glass layer, we were
} wondering whether we could use a staining procedure.
}
} For instance, we are aware of a wet chemical procedure allowing to
} tin-plate copper. However, without trying it we don't know if it would
} result in a considerable different contrast in the SEM as the atomic
} mass of Sn is very close to the one of Cu.
} Wet chemical gold plating on the other hand involves not only expensive
} but also highly toxic chemicals.
}
} Are you aware of (wet chemical) staining procedures for copper that
} produce a considerable contrast and are preferably inexpensive as well
} as non highly toxic ?
}
} Is there maybe another technique we could use ?
}
} Kind regards,
} Stefan
}
} ==============================Original Headers==============================
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From: CGorman-at-hookecollege.com
Date: Mon, 12 Sep 2016 09:15:44 -0500
Subject: [Microscopy] Transmission Electron Microscopy Short Course

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings,

Hooke College of Applied Sciences, located in Westmont, IL, is offering a Transmission Electron Microscopy short course October 11-13, 2016. In addition to lectures, this course emphasizes hands-on training using state-of-the-art equipment.

For further TEM training details and registration information, please follow the link below:

https://www.mccrone.com/transmission-electron-microscopy-tem-course

Chris Gorman
Hooke College of Applied Sciences, LLC .850 Pasquinelli Drive.Westmont, IL 60559



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From: drk-at-shcc.org
Date: Mon, 12 Sep 2016 14:39:22 -0500
Subject: [Microscopy] Pacific Northwest Microscopy Society Symposium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings Fellow Microscopists,

The Pacific Northwest Microscopy Society will hold its 2016 Fall symposium on Thursday November 3rd, continuing Friday November 4th. The program will showcase innovative area scientists pioneering the latest technologies in microscopy. Both invited and contributed talks will be presented at the Portland Shriners Hospital for Children. Workshops will be conducted at the nearby Center for Spatial Systems Biology at the new Oregon Health Sciences University waterfront campus. Here is an opportunity to interact with those at the forefront of emerging technologies in a friendly atmosphere with many opportunities for conversation and collaboration. The preliminary program and registration can be found on the Pacific Northwest Microscopy Society webpage at:

http://pacificnwmicroscopy.org

Please plan to attend this informative program and meet with your local coleagues and vendors.

Thank you,

Doug



Douglas R. Keene
Assistant Investigator
Micro-Imaging Center
Shriners Hospital for Children
3101 SW Sam Jackson Park Road
Portland, Oregon 97239
Office: 503-221-3434
Mobile: 503-819-3600



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From: vray-at-partbeamsystech.com
Date: Tue, 13 Sep 2016 11:18:57 -0500
Subject: [Microscopy] Re: Staining of Cu for SEM imaging

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Hi Stefan,

You are correct that negative biasing of the sample would decelerate
primary electrons thus reducing landing energy and penetration depth,
but it would take much higher then the 10V - more like a few kV of
negative bias.

But here is another bias idea which may work with potentials in 10V
range. When you collecting images from areas of Cu covered by
dielectric, not only the primary electron beam penetrates the glass, but
also secondary electrons must penetrate the same layer of glass to
escape. There is electron beam induced conductivity that helps them,
but... if you apply small *positive* bias to your sample, it would hold
down secondary electrons. Dielectric layer, however thin, would act as
additional filter for the secondaries coming from Cu covered by the
glass. It is very likely that you would find "sweet spot" where
combination of positive bias and attenuation from thin layer of glass
would made covered areas of Cu visibly darker then the open areas.....

Valery

Valery Ray
www.linkedin.com/in/valeryray
==============================
PBS&T, MEO Engineering Company
290 Broadway, Suite 298
Methuen, MA 01844, USA
Phone: +1-978-305-0479 - leave a message
Mobie: +1-978-305-0479 - leave a message
E-mail: vray-at-partbeamsystech.com
Web: www.partbeamsystech.com
Web: www.freudlabs.com

On 9/13/2016 5:00 AM, Stefan Schoenleitner wrote:
} Dear Valery,
}
} first of all I would like to thank you for your extensive list of tips.
} This is definitely encouraging and provides me with a bunch of
} techniques I could try and evaluate.
}
} On 09/11/2016 07:45 AM, Valery Ray wrote:
} } Hi Stefan,
} }
} } There are few things you can try to distinguish between exposed and
} } slightly-buried Cu conductors:
} }
} } 1. Based on your mentioning of "atomic mass of Sn is very close to the
} } one of Cu" I am suspecting you may be using backscattered electrons for
} } imaging - try secondary electron detector imaging at lowest
} } acceleration you can afford without loosing too much resolution.
}
} That was my error. I looked at the periodic table and looked up "Zn"
} instead of "Sn" ;)
} Usually I like to use the BSE since it allows me to differentiate
} between different materials. However, as our SEM is rather old, the BSE
} detector is rather insensitive so that I can't go below 10kV.
} Yet, even at 5kV the electron beam penetration depth for SiO2 is roughly
} 300nm while my glass layers are probably around 100nm.
} As a result, even with the SE detector, I can see through.
} In fact, this is not a problem as long as I can see the difference of
} exposed copper on the surface and buried copper below the surface.
}
} } 2. Apply negative bias to the sample (retarding field) to reduce landing
} } energy of primary electrons. This could work or not, depending on
} } geometry of your instrument, stage, and your instrumentation
} } skills/capabilities.
}
} I have an electrical feed through. So the idea would be to bias the
} sample with a negative voltage in relation to the GND of the SEM so that
} the electrons from the beam are effectively decelerated, right ?
} What kind of voltages should I try for that ? Is a couple of volts
} ( {10V) sufficient or does it typically have to be more ?
}
} } 3. Electroless nickel plating chemicals cost less then Au plating kits,
} } though either one is safe enough to do in ordinary fume hood with gloves
} } and common sense.
}
} What are the advantages and disadvantages of different plating metals ?
} Apparently the options are at least tin-plating, nickel-plating and
} Au-plating ?
} So my naive approach would be to choose a metal with an atomic mass as
} far away from Cu as possible.
} I suppose the difference is the thickness of the plating which might
} indirectly have an influence on the resolution meaning that copper
} structures with a small diameter (i.e. 100nm) would probably either be
} not coated or coated with excessive amounts of material ?
}
}
} } 4. Exposure to iodine vapor in humid atmosphere (room air) would corrode
} } exposed Cu fairly quickly, making it look "fluffy" or "rough," while
} } buried conductors will remain intact for a while. The tricky part would
} } be to image/scan the sample fairly quickly - once started the corrosion
} } would eventually consume all the Cu layers. You want to image exposed
} } layer once it has been just so slightly corroded on the surface, and
} } immediately polish it away to prevent damage for the underlying layers.
}
} Do you have an example SEM image how that would look like ?
} Wouldn't the etching process stop rather quickly as soon as the sample
} is no longer exposed to the vapor ? Could it be rinsed ?
} I think I would prefer a wet chemical approach if possible.
}
} } 5. Either sulfuric or nitric acid with H2O2 should etch Cu
} } crystallographically, making exposed surface look differently then metal
} } protected by dielectric. You would want to experiment with "disposable"
} } devices and develop reliable procedure prior to immersing "real" one.
} } Same is true for plating, by the way.
}
} Sure.
}
} } 6. Image the chip, etch away Cu with FeCl3, wash-rinse, and the image
} } same area again.
}
} Is there a reason for using FeCl3 ? So far I performed copper etches
} either with HCl+H2O2 or with low HNO3 concentrations.
}
} } Buried under dielectric Cu would be on both images,
} } while exposed would disappear after etching. You would need to develop
} } procedure and control time/temperature/concentration of etchant to get
} } repeatable results.
}
} OK, that would be a differential approach where we take image two times,
} align the images and create a difference image. Could work ;)
}
}
} } 7. Do forward-scattered imaging, or even regular Se imaging at glancing
} } angle - it would be much more sensitive to presence of thin dielectric
} } layer. Foreshortening correction would be required....
}
} I will try imaging at an angle. Sounds interesting !
}
} } Happy Imaging :)
}
} Thank you so much !
}
} Stefan
}
} }
} } Valery
} }
} }
} } Valery Ray
} } www.linkedin.com/in/valeryray
} } ==============================
} } PBS&T, MEO Engineering Company
} } 290 Broadway, Suite 298
} } Methuen, MA 01844, USA
} } Phone: +1-978-305-0479 - leave a message
} } Mobie: +1-978-305-0479 - leave a message
} } E-mail: vray-at-partbeamsystech.com
} } Web: www.partbeamsystech.com
} } Web: www.freudlabs.com
}
}

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6, 39 -- From: Valery Ray {vray-at-partbeamsystech.com}
6, 39 -- Subject: Re: [Microscopy] Staining of Cu for SEM imaging
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From: microscopy.listserver-at-gmail.com
Date: Tue, 13 Sep 2016 17:23:44 -0500
Subject: [Microscopy] viaWWW:Microscopy Technologist Positions at Sandia Livermore

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Email: jdsugar-at-sandia.gov Name: Josh Sugar

Organization: Sandia National Labs

Title-Subject: [Filtered] Microscopy Technologist Positions at Sandia Livermore

Message: (Apologies to the microscopy community, previous post had bad links, these should be better.)
Sandia National Labs is looking for experienced and entry level microscopy technologists. Please see
the job using the links below or searching for the Job ID in the Sandia careers website. Please do
not email me directly, applications must be submitted through the website to be considered.

Job Opening ID: 654869
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From: microscopy.listserver-at-gmail.com
Date: Tue, 13 Sep 2016 17:24:26 -0500
Subject: [Microscopy] viaWWW:Electron Microscopy Sciences sponsors Fall 2016 Workshop

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Email: stacie-at-ems-secure.com Name: Stacie Kirsch

Organization: Electron Microscopy Sciences

Title-Subject: [Filtered] Electron Microscopy Sciences sponsors Fall 2016 Workshop

Message: Aurion and Electron Microscopy Sciences are pleased to announce they will hold another of
their immensely popular workshops on ImmunoGold Silver Staining at the University of Maryland,
Baltimore, Core Imaging Facility, from October 19-21, 2016.
The course objectives are to provide researchers the opportunity to learn the theory and practice of
immunogold labeling and to promote technology exchange and research collaboration by allowing
participants to process their own samples under expert guidance.
The ImmunoGold Silver Staining Workshop is designed and taught by Mr. Peter van de Plas who has been
with Aurion since 1991. Mr. van de Plas worked closely together with Dr. Leunissen in founding a
firm basis for Aurion including development of product applications. He has been invited to many
international microscopy conferences and workshops and is especially experienced in providing
hands-on training. The Electron Microscopy Core Imaging Facility (EMCIF) provides electron
microscopy related research and imaging services to all faculty and staff of the University of
Maryland campus and the academic and industrial community in the Washington D.C. and Baltimore areas.
The three day course will cover the properties of gold particles and their protein conjugates,
theories underlying immunogold labeling protocols, and silver enhancement of gold particles. Also
covered in the course will be immunogold labeling on a variety of sample preparations for LM,
immunogold labeling for EM, pre/post-embedding double immunogold labeling, and background
minimization in immunogold labeling.
Please visit the course webpage for additional information, as well as to register and pay online,
at www.emsdiasum.com/microscopy/products/immunogold/workshop.aspx. Or download our brochure at
www.emsdiasum.com/workshop. Registration is limited to 16 participants.
Event contact: Stacie Kirsch, stacie-at-ems-secure.com, 800-523-5874.


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From: microscopy.listserver-at-gmail.com
Date: Wed, 14 Sep 2016 07:42:56 -0500
Subject: [Microscopy] viaWWW:Cryo TEM - Blotting with filter paper

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Email: lpq023-at-mail.com Name: PQ

Organization: -

Title-Subject: [Filtered] Cryo TEM - Blotting with filter paper

Message: Dear all,

I have a question with regards to cryo TEM sample preparation.

I was wondering, what will be the effect of different filter papers used for blotting the samples?
From what I know, the biggest difference between filter papers is their particle retention. Is
this a critical criteria when it comes to blotting the samples?

Also, most papers report I come across usually report using Whatman Grade #1 filter papers to do the
blotting. Currently, I only have access to Advantech Grade #1 filter papers. Will this affect the
result of my blotting and sample preparation?

I am a beginner in terms of the cryo TEM method and would really like to work on my technique to
better my cryo TEM sample preparation skills. Any feedback/comments/suggestions/advice would be
greatly appreciated.

Thank you in advance!

Sincerely,
PQ

P.S. I am not sure if it could be relevant, but just in case, I am using a Gatan cryoplunge for my
sample preparation.

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From: aheiss-at-amnh.org
Date: Wed, 14 Sep 2016 08:59:47 -0500
Subject: [Microscopy] Help with Focussing a Zeiss EM109

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Hello all! Our facility has a secondhand Zeiss EM109 TEM, which we've recently had serviced. Part of this servicing included getting the coils adjusted mechanically, and it should be in peak condition. However, I have a lot of difficulty getting things into clear focus. I've gotten (I think) quite experienced with aligning and stigmating it, but that doesn't seem to fix things -- indeed, after capturing a blurry image and realigning, the next image of the same sample often looks worse. I suspect that the stigmation is off, but I'm not sure; often I can get better results by systematically capturing images at different focus settings (though of course even with a digital camera this takes a long time, and doesn't do the specimen any favours). Are there any tricks to this?

One question that I have in particular concerns the projector stigmation. I set this using the caustic, which I try to get to look like a Mercedes-Benz symbol (a perfectly symmetrical three-pointed star in a circle). However, changing the low-mag focus (the taller inner knob that doesn't click) changes the appearance of the caustic, such that at one focus setting (say, with the caustic spread out) the caustic is symmetrical, and at another (in this case when it's smaller) it isn't. Is there a "sweet spot" that I should have that focus set to (i.e., should I make the caustic as small as possible while still visible, or as large)?

I should note that I have experimented a little with the stigmation while viewing specimens, and haven't noticed any effect (unlike my experience with a Tecnai-12, on which I could correct the astigmatism just looking at the image). But I do suspect there is a stigmation problem somewhere in the scope, and just need to know how to fix it.

Any advice on the care and feeding of this 'scope would be much appreciated.

Thanks!
Aaron


Aaron A. Heiss, Ph.D.
Research Scientist and Simons Foundation Fellow
Eunsoo Kim Laboratory
Department of Invertebrate Zoology
American Museum of Natural History
Central Park West at 79th Street
New York, NY 10024 USA

212-769-5838
aheiss-at-amnh.org


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From: Z.Zhou-at-lboro.ac.uk
Date: Wed, 14 Sep 2016 09:04:10 -0500
Subject: [Microscopy] TEM or SEM---solutions to record movies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Fellow Microscopist,

What are the solutions you have to record videos on the microscope? I'm using a Tecnai F20 (S)TEM with Gatan DM1.83.842, slow scan CCD 1kx1k 794. I'm open to all sorts of ways to capture videos during the observation, either by software to capture the screen display, or by DM scripts.
All the best,
Zhou

Dr Z Zhou
Research Fellow
Loughborough Materials Characterisation Centre (LMCC)
Department of Materials
Loughborough University
Leicestershire
LE11 3TU

Tel: 01509 223163
Fax: 01509 234225





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8, 63 -- Subject: TEM or SEM---solutions to record movies
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From: protrain-at-emcourses.com
Date: Thu, 15 Sep 2016 03:15:54 -0500
Subject: [Microscopy] Help with Focussing a Zeiss EM109

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Aaron

It is very many years since I used a Zeiss 109, so my memory of its layout
is long gone, but I may be able to help you?
The image in any TEM is created "in focus" by interaction between the
objective lens and the lens which immediately follows it. Any problem with
the clarity of this image will be related to the objective lens setting and
the setting of the objective lens stigmators. Problems in any other lens
(other than instabilities) will not have any influence on the image
sharpness.
You say that the instrument has been serviced by a person who knows the 109,
so we must expect it to be free of high voltage or lens problems. That
brings us back to the objective lens, but most of all in your case, the
objective astigmatism. You note that with a through focal series the image
improves, this goes hand in hand with astigmatism. At true focus the
astigmatism will be minimised but as you move out of focus it will destroy
the image quality.
Please look into the objective stigmator, it may be a double strength system
like all modern instruments, or it may be in the style of early instruments.
Early instruments used a stigmator which provided a device to change the
stigmator field strength, combined with the ability to change the direction
of that field. This system was called strength and rotate and it may be the
style on the 109. From the point of best focus change the strength control
until you see it change the image. Then change the rotate control until you
find the point that improves the image quality. Then back off the strength
slightly, check the rotation, check the focus. Repeat this procedure until
the image is satisfactory.

Best of luck with your problem.

Steve

Steve Chapman FRMS
Protrain for Consultancy and Training in Electron Microscopy
+44 (0)7711 606967
web www.emcourses.com


-----Original Message-----
X-from: aheiss-at-amnh.org [mailto:aheiss-at-amnh.org]
Sent: 14 September 2016 15:01
To: protrain-at-emcourses.com

Hello all! Our facility has a secondhand Zeiss EM109 TEM, which we've
recently had serviced. Part of this servicing included getting the coils
adjusted mechanically, and it should be in peak condition. However, I have
a lot of difficulty getting things into clear focus. I've gotten (I think)
quite experienced with aligning and stigmating it, but that doesn't seem to
fix things -- indeed, after capturing a blurry image and realigning, the
next image of the same sample often looks worse. I suspect that the
stigmation is off, but I'm not sure; often I can get better results by
systematically capturing images at different focus settings (though of
course even with a digital camera this takes a long time, and doesn't do the
specimen any favours). Are there any tricks to this?

One question that I have in particular concerns the projector stigmation. I
set this using the caustic, which I try to get to look like a Mercedes-Benz
symbol (a perfectly symmetrical three-pointed star in a circle). However,
changing the low-mag focus (the taller inner knob that doesn't click)
changes the appearance of the caustic, such that at one focus setting (say,
with the caustic spread out) the caustic is symmetrical, and at another (in
this case when it's smaller) it isn't. Is there a "sweet spot" that I
should have that focus set to (i.e., should I make the caustic as small as
possible while still visible, or as large)?

I should note that I have experimented a little with the stigmation while
viewing specimens, and haven't noticed any effect (unlike my experience with
a Tecnai-12, on which I could correct the astigmatism just looking at the
image). But I do suspect there is a stigmation problem somewhere in the
scope, and just need to know how to fix it.

Any advice on the care and feeding of this 'scope would be much appreciated.

Thanks!
Aaron


Aaron A. Heiss, Ph.D.
Research Scientist and Simons Foundation Fellow Eunsoo Kim Laboratory
Department of Invertebrate Zoology American Museum of Natural History
Central Park West at 79th Street New York, NY 10024 USA

212-769-5838
aheiss-at-amnh.org


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21, 22 -- Subject: RE: [Microscopy] Help with Focussing a Zeiss EM109
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From: wij.muss-at-aon.at
Date: Thu, 15 Sep 2016 07:23:02 -0500
Subject: [Microscopy] Help with Focussing a Zeiss EM109

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Dr. Aaron A. Heiss.

To answer your question more precisely it would be interesting to know at
{which magnification level [ZEISS TEM109 ....the old analog= I,II,III+) or
still a recently digitalized version? } you have the focusing problems.
This might be combined also with your problem concerning the "caustic
figure" :
["However, changing the low-mag focus (the taller inner knob that doesn't
click) changes the appearance of the caustic ++), such that at one focus
setting (say, with the caustic spread out) the caustic is symmetrical, and
at another (in this case when it's smaller) it isn't.]

+) I ="low-mag level": Range x 150 - x 1100; II = "middle-mag level" = x
3,000 - x 20,000; III = "High mag level"= x 30,000 - x250,000 and (HM) =
x 400,000
++) that's right.... but focusing at the middle-, and high- mag-level as
compared to focusing at the low-mag level is another { { procedure/another
"knob" } } if I remember correctly....


Normally, if "my" TEM would have been serviced (as yours) and I can't get
easily {micrographs in focus} at any mag-level - I am sure I would have {
seriously invited again } the service man coming in again to have a look
into the machine and poti's (potentiometers) again
(not knowing whether your 109 is in classical (analog) or a modernized
fashion. It would also be interesting IF you own a - and if yes, WHICH -
Operating Manual you have to reread the focusing procedure which for the
low-mag-level is different from the middle-mag-level).
If you could upload some images of the "knobs" etc. it would be of benefit
in order to help you...
(admitting that I have worked with a ZEISS-TEM109 vintage 1978 until 2015
without any focusing problem you described).


Regards and best wishes,
Wolfgang MUSS PhD.
Emeritus member of MSA
SALZBURG, Austria



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-----Ursprngliche Nachricht-----
Von: protrain-at-emcourses.com [mailto:protrain-at-emcourses.com]
Gesendet: Donnerstag, 15. September 2016 11:08
An: wij.muss-at-aon.at
Betreff: [Microscopy] RE: Help with Focussing a Zeiss EM109
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Hi Aaron

It is very many years since I used a Zeiss 109, so my memory of its layout
is long gone, but I may be able to help you?
The image in any TEM is created "in focus" by interaction between the
objective lens and the lens which immediately follows it.
Any problem with the clarity of this image will be related to the objective
lens setting and the setting of the objective lens stigmators. Problems in
any other lens (other than instabilities) will not have any influence on the
image sharpness.

You say that the instrument has been serviced by a person who knows the 109,
so we must expect it to be free of high voltage or lens problems.
That brings us back to the objective lens, but most of all in your case, the
objective astigmatism. You note that with a through focal series the image
improves, this goes hand in hand with astigmatism.
At true focus the astigmatism will be minimised but as you move out of focus
it will destroy the image quality.

Please look into the objective stigmator, it may be a double strength system
like all modern instruments, or it may be in the style of early instruments.
Early instruments used a stigmator which provided a device to change the
stigmator field strength, combined with the ability to change the direction
of that field.
This system was called strength and rotate and it may be the style on the
109. From the point of best focus change the strength control until you see
it change the image.
Then change the rotate control until you find the point that improves the
image quality.
Then back off the strength slightly, check the rotation, check the focus.
Repeat this procedure until the image is satisfactory.

Best of luck with your problem.

Steve

Steve Chapman FRMS
Protrain for Consultancy and Training in Electron Microscopy
+44 (0)7711 606967
web www.emcourses.com


-----Original Message-----
X-from: aheiss-at-amnh.org [mailto:aheiss-at-amnh.org]
Sent: 14 September 2016 15:01
To: protrain-at-emcourses.com

One question that I have in particular concerns the projector stigmation. I
set this using the caustic, which I try to get to look like a Mercedes-Benz
symbol (a perfectly symmetrical three-pointed star in a circle). However,
changing the low-mag focus (the taller inner knob that doesn't click)
changes the appearance of the caustic, such that at one focus setting (say,
with the caustic spread out) the caustic is symmetrical, and at another (in
this case when it's smaller) it isn't. Is there a "sweet spot" that I
should have that focus set to (i.e., should I make the caustic as small as
possible while still visible, or as large)?

I should note that I have experimented a little with the stigmation while
viewing specimens, and haven't noticed any effect (unlike my experience with
a Tecnai-12, on which I could correct the astigmatism just looking at the
image). But I do suspect there is a stigmation problem somewhere in the
scope, and just need to know how to fix it.

Any advice on the care and feeding of this 'scope would be much appreciated.

Thanks!
Aaron


Aaron A. Heiss, Ph.D.
Research Scientist and Simons Foundation Fellow Eunsoo Kim Laboratory
Department of Invertebrate Zoology American Museum of Natural History
Central Park West at 79th Street New York, NY 10024 USA

212-769-5838
aheiss-at-amnh.org


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From: microscopy.listserver-at-gmail.com
Date: Thu, 15 Sep 2016 07:38:20 -0500
Subject: [Microscopy] viaWWW:Spring clamp ring - TEM FEI single tilt holder

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Email: ravi.thakkar369-at-gmail.com Name: Thakkar

Title-Subject: [Filtered] Spring clamp ring - TEM single tilt holder.
Message: Hi All,
A ring of spring clamp in FEI single tilt holder is coming out very frequently due to one of the arm
of spring clamp is loosen. I am afraid, it may fall apart in near future. Unfortunately our TEM is
not cover with contract currently.
Is there any home remedy to fix this problem ?

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From: microscopy.listserver-at-gmail.com
Date: Thu, 15 Sep 2016 18:51:03 -0500
Subject: [Microscopy] viaWWW: eliminating section folds

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Email: marie.cantino-at-uconn.edu Name: Marie Cantino

Organization: University of Connecticut, Biosciences Electron Microscopy Laboratory

Title-Subject: [Filtered] section folds

Message: Several people asked me to report on what methods worked best for us, after I posted a
question about eliminating section folds. I posted a summary of the responses we got, but had to
wait for our microtomy expert to return and answer this question. Here is the response from Dr.
Maritza Abril in our lab:
"I tested the tips generously provided for the trimming, microtomy, picking up sections from the
knife boat and for drying the sections on the glass slide as our samples were already fixed and
embedded. I tried individual suggestions as well as several combinations including: cutting thinner
(0.5 m) sections; applying solvent vapors (trichloroethylene and chloroform) to sections still in
the knife boat by waving a Q-tip over the sections; using untreated slides, trimming away the excess
resin; placing a few drops of acetone on a Q-tip on the frosted end of the slide and covering the
slide with a Petri dish until the water drop containing the section dried; adding 1% ethylene glycol
to the water drop on the slide; decreasing/increasing the temperature (70C/ 90C) or drying at room
temperature; and staining at 70C and 90C. These are the techniques that minimized wrinkles. A
word of caution, when drying untreated slides at room temperature make sure to reheat after all
water and/or solvent is gone to ensure adhesion of the sections.
The optimal minimization of wrinkles was obtained by cutting 1 m thick sections, trimming away the
empty resin on all sides of the block (as we could not make the block smaller since we were looking
at a tobacco graft), and picking up the sections on 10% acetone (instead of distilled water) on
Superfrost Plus slides. Sections were dried by putting the slide, section side down, over a small
open Petri dish (previously heated) to evaporate on the hotplate. Once dry they were placed
directly on the hotplate, section side up, and stained at 70C."


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From: microscopy.listserver-at-gmail.com
Date: Sat, 17 Sep 2016 08:07:41 -0500
Subject: [Microscopy] viaWWW:] MPFI EM workshop 2016 - CLEM workflows

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Email: naomi.kamasawa-at-mpfi.org Name: Naomi Kamasawa

Organization: Max Planck Florida Institute for Neuroscience

Title-Subject: [Filtered] MPFI EM workshop 2016 - CLEM workflows -

Message: The Electron Microscopy Core Facility from Max Planck Florida Institute for Neuroscience
will host this exciting 2-day workshop, providing you with educational lectures by experts in the
field and hands-on training that you can adapt to your own projects.
Dates: November 2-3, 2016

Workshop Highlights 1. Presentations by specialists reviewing CLEM technologies and applications
2. Casual reception with specialists for Q & A
3. Interactive tour of EM Core facilities and brief demo of the latest equipment
4. Hands-On Session (2nd Day) for ATUMtome (RMC/Boeckeler), Atlas5 AT with Merlin VP Compact &
Gemini SEM300 (Carl Zeiss), and others.
Registration Deadline; October 13, 2016

Registration fees; First day lectures only, Free
Lectures and hands-on demonstration (Limited to 12 seats), $30

Please see details;
https://www.maxplanckflorida.org/education/courses/EM-course/EMC-2016/

Contact; Naomi Kamasawa (Head, Electron Microscopy Facility)
naomi.kamasawa-at-mpfi.org

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From: lamiller-at-illinois.edu
Date: Mon, 19 Sep 2016 07:28:04 -0500
Subject: [Microscopy] Invitation to Fall Biological Conference and Student Competition at

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Greetings!

The University of Illinois at Urbana-Champaign, Materials Research Laboratory is putting on it’s annual Biological Fall Conference on November 2-3rd. The title this year is “Diverse Fields of Study.. Steps Toward Medicine"

Everyone is invited to come, the registration is only $25 (for food) and it includes a Vendor Fair, Student Competition, Tours and a whole line up of speakers.


We have speakers on hydrogel printing, nano gold research, prosthetic hands that move and can feel pressure, bone implants etc.


Registration, directions to be up shortly, competition rules and more information can be found at:
http://mrl.illinois.edu/mrl-biological-conference-2016


The student competition is open to all academia! We also have a division in the competition for post docs.

Simply have a topic that involves something biological, can use some of the same instruments as are found in our core facilities, talk for 15 minutes and present a poster. Those competing will get their registration fee refunded.
Winners take home $99 first place, $50 second place and have their image on our website for several months.

Registration for the competition is part of the regular registration for the conference. Upload a half page abstract during the registration. DEADLINE FOR REGISTRATION to compete is OCTOBER 1st.



Hope you can come!


Lou Ann






{ { { { { { { { { { { { { { { { {} } } } } } } } } } } } } } } }
Lou Ann Miller
Biological Electron Microscopy
Frederick Seitz Materials Research Laboratory
Lab Room 125 Office 374 MRL
104 South Goodwin Ave
Urbana, IL 61801
Campus mail code: MC-230
Hours: 7am-3:30 pm

217-244-1567
lamiller-at-illinois.edu
http://mrl.illinois.edu



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25, 43 -- From: "Miller, Lou A" {lamiller-at-illinois.edu}
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From: microscopy.listserver-at-gmail.com
Date: Mon, 19 Sep 2016 07:46:31 -0500
Subject: [Microscopy] viaWWW:Transition from Biological to Material science EM?

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Email: Desertrat99-at-verizon.net Name: Eric Rosen

Organization: UCLA

Title-Subject: [Filtered] Transition from Biological to Material science EM?
Message: Hi all,
Curious to know how difficult it would be to transition from Bioogical Em to Material Science?
I have want d to learn Material science EM for a while but there are no courses offered down here in
the LA area I know of.

Thanks.
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From: microscopy.listserver-at-gmail.com
Date: Mon, 19 Sep 2016 15:01:10 -0500
Subject: [Microscopy] viaWWW:JEOL 840 with JEOL Orion software

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Email: gary-at-cermetmaterials.com Name: Gary Castelow

Organization: CERMET MATERIALS INC.

Title-Subject: [Filtered] JEOL 840 with JEOL Orion software

Message: We acquired an upgrade (from our T300) that was working when we bought it. We cannot get
the software to work though. From what we can tell all connections seem correct. When we click to
load the Orion program the main loading screen pops up "Loading imaging system" then the computer
completely and only a hard reset gets it going again. Even the keyboard locks up (caps/numbers lock
does nothing). We are running Windows XP and version 1.72.08 Any help is greatly appreciated!!

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From: microscopy.listserver-at-gmail.com
Date: Wed, 21 Sep 2016 08:02:29 -0500
Subject: [Microscopy] viaWWW:Job opening at UC Riverside

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Email: bozhilov-at-ucr.edu Name: Krassimir Bozhilov

Organization: University of California

Title-Subject: [Filtered] Job opening at UC Riverside

Message: The Central Facility for Advanced Microscopy and Microanalysis (CFAMM) at the University of
California at Riveside has an open position for electron microscopy laboratory technician. Position
responsibilities include operation and routine maintenance of electron microscopes, FIB, ancillary
equipment, and all phases of sample preparation and analysis of various types of materials and
tissue. This position includes assisting and training facility users in specimen preparation,
instrument operation, and related techniques.

To apply for the job visit:
https://irecruitportal.ucr.edu/irecruit/!Controller?action=jobs_webui.show_page&page=jobs_browser&public=true&module=jobs.

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From: microscopy.listserver-at-gmail.com
Date: Wed, 21 Sep 2016 08:03:30 -0500
Subject: [Microscopy] viaWWW:Job opening Confocal Sales Specialist

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X-from: ml-at-personifysearch.com

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Email: ml-at-personifysearch.com Name: Mack Lloyd
Organization: Personify

Title-Subject: [Filtered] Confocal Sales Specialist
Message: Hello,
I am working on a new job Opportunity with a World Leading Microscopy company. The primary function
of this position is to execute a sales plan and recommend strategies to improve the sale of Confocal
Products in a territory that includes: Pittsburgh, Michigan, West Virginia, Virginia and Ohio.
Please see the link to the job below and respond to ml-at-personifysearch.com if you have any questions.

Thanks!!

Mack Lloyd
https://danaher.taleo.net/careersection/jobdetail.ftl?job=LEI002808&lang=en#.V-KBPIVBGF4.google


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From: bozhilov-at-ucr.edu
Date: Wed, 21 Sep 2016 10:28:23 -0500
Subject: [Microscopy] Staff microscopy technician position at UC Riverside

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The Central Facility for Advanced Microscopy and Microanalysis (CFAMM) at the University of
California at Riveside has an open position for electron microscopy laboratory technician. Position
responsibilities include operation and routine maintenance of electron microscopes, FIB, ancillary
equipment, and all phases of sample preparation and analysis of various types of materials and
tissue. This position includes assisting and training facility users in specimen preparation,
instrument operation, and related techniques.

To apply for the job visit:
https://irecruitportal.ucr.edu/irecruit/!Controller?action=jobs_webui.show_page&page=jobs_detail&requisition_id=201609161802&profile_id=&module=jobs

The job number in the listing is : 201609161802.


////////////////////////////////////////////////////////////////////////////////
Krassimir N. Bozhilov

Central Facility for Advanced Microscopy and Microanalysis - Research and Econ. Development Office
Materials Science & Engineering - BCOE
University of California
Riverside, CA 92521

bozhilov-at-ucr.edu
tel. 951 827 2998
fax 951 827 2489
////////////////////////////////////////////////////////////////////////////////

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From: jkrupp-at-deltacollege.edu
Date: Wed, 21 Sep 2016 15:28:39 -0500
Subject: [Microscopy] Best kind of bottles?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

No laughing, I am curious to know what is the best kind of bottle/container for various EM stuff.

I have always been a glass kind of guy. Thought plastic might have issues with some chemicals and I have had very old plastic bottles get brittle and go to pieces on the shelf.

But, I am willing to reconsider. Plastic is lighter, maybe cheaper, and less breakable while young.

Is plastic OK for most things. I’m thinking about storing stock solutions of buffers, acetone, alcohol, fixatives etc.

I need to buy some new supplies and am wondering which way to go. Not exactly the paper or plastic question at the grocery store, but what are your thoughts?

Jon

Jonathan Krupp
ASB&T
San Joaquin Delta College
5151Pacific Ave.
Stockton, CA 95207
(209) 954-5284
jkrupp-at-deltacollege.edu



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From: microscopy.listserver-at-gmail.com
Date: Thu, 22 Sep 2016 07:21:53 -0500
Subject: [Microscopy] viaWWW:NZ Microscopy Conference - 31 Jan - 3 Feb 2017

Contents Retrieved from Microscopy Listserver Archives
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Email: LindaG-at-adhb.govt.nz Name: Linda Graham

Organization: LabPlus

Title-Subject: [Filtered] NZ Microscopy Conference - 31 Jan - 3 Feb 2017

Message: Notice of Conference on behalf of the Conference Committee.

Registration and abstract submission are now open for this upcoming 2017 conference with more
details on the conference website www.microscopy2017.co.nz

There are a number of interesting workshops being held on the day before the conference proper.
These workshops have limited numbers so please register early if you are interested in coming to a
workshop.

We have pleasure in confirming our invited international speakers listed below;

Professor Julie Cairney, University of Sydney, Materials at the atomic level

Scientia Professor Katharina Gaus, University of New South Wales, Super-resolution and single
molecule science

Dr Arun Sampathkumar, Max Planck Institute of Molecular Plant Physiology, Plant cell biology

Dr Lucia Burgio, Department of Conservation Science, Victoria and Albert Museum, Analysis and
technical examination of museum objects, with Raman microscopy, X-ray fluorescence and optical
microscopy

Dates: Workshops 31st January 2017. Conference: 1-3 February 2017. Venue AUT, Auckland, NZ.


Dr Ian Hallett
Conference Convener - Microscopy 2017

Senior Scientist
Team Leader - Microscopy and Cell Walls


T: +64 9 925 7027
F: +64 9 925 7001
ian.hallett-at-plantandfood.co.nz
www.plantandfood.co.nz
The New Zealand Institute for Plant & Food Research Limited


Regards
Linda M Graham
Electron Microscopy Technical Specialist
Anatomical Pathology
LabPlus
Phone 09 3737 599 (or 9090 from A+) extension 86474 or 86052
Mobile: 021 915025
email: lindag-at-adhb.govt.nz

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From: microscopy.listserver-at-gmail.com
Date: Thu, 22 Sep 2016 07:22:37 -0500
Subject: [Microscopy] viaWWW:B field strength in JEOL 2100FX

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X-from: hasan.ali-at-angstrom.uu.se

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Email: hasan.ali-at-angstrom.uu.se Name: Hasan Ali

Organization: Uppsala University

Title-Subject: [Filtered] B field strength in JEOL 2100FX

Message: We are looking for the B field strength in a JEOL 2100.

Do you know someone who might have measured it or have a value that is at least by 10% close to the
real value?
Thanks

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From: wim.hagen-at-me.com
Date: Thu, 22 Sep 2016 10:47:15 -0500
Subject: [Microscopy] Re: viaWWW:B field strength in JEOL 2100FX

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Dear Hasan,

See CHRISTENSON, K., & EADES, J. (1986). On “parallel” illumination in the transmission electron microscope. Ultramicroscopy, 19(2), 191–194. http://doi.org/10.1016/j.ultramic.2006.04.029

You could do the experiment described in this paper to measure the Larmor frequency. With near-parallel illumination, take a series of images over a large Z range, then align the images in e.g. MIDAS which is part of IMOD software (http://bio3d.colorado.edu/imod/), this will give the image rotation per focus step used, the Larmor frequency.. Once you have the Larmor frequency, you can calculate the field strength in the immersion lens.

This paper from the same authors has a bit more detail: CHRISTENSON, K., & EADES, J. (1988). Skew Thoughts on Parallelism. Ultramicroscopy, 26, 113–132.

Best,

Wim Hagen
EMBL Heidelberg

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} Title-Subject: [Filtered] B field strength in JEOL 2100FX
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From: microscopy.listserver-at-gmail.com
Date: Fri, 23 Sep 2016 18:42:50 -0500
Subject: [Microscopy] viaWWW:CryoJane Slides - how to attach the sections to slides?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Wim,

Thanks for the reply and nice information.

Best Regards
Hasan Ali
________________________________________
X-from: Wim Hagen [wim.hagen-at-me.com]
Sent: Thursday, September 22, 2016 5:48 PM
To: Microscopy-at-microscopy.com; Hasan Ali

Oh Pamela,

Your question raised the hair on the back of my irony bone.
I have never chipped a Coplin jar, because I always clean them by hand.
Thus, if one shows up with a chip, I assume it is feeling suicidal.
I did, once, try to repair a chipped Coplin, but it was insistent and just chipped elsewhere.
I can clearly recall that day, oh so long ago, when I finally gave up and ordered a case of the things.
There are few items in the armamentum of the histologists lab that can be as ornery as chippy Coplin jars.
I can still remember the days when I gave up and went home early.

On the practical side, I can only offer advices that I will not put in print, other than to say,
"Don't bump them on anything anymore! They must feel pain or aggravation about the way they are treated in the workplace. Or, it might be that they are upset by failing to have authority over only 10 slides, and often only ONE at a time. It's a wonder that they haven't unionized."

Wishing you luck,

Fred Monson

Frederick C. Monson, PhD
Technical Director
Center for Microanalysis and Imaging, Research and Training (CMIRT)
West Chester University of PA
Geology-Astronomy
750 South Church St.
West Chester, PA, 19383
fmonson-at-wcupa.edu
610-738-0437(Work)



-----Original Message-----
X-from: Romundstad, Pamela K via Histonet [mailto:histonet-at-lists.utsouthwestern.edu]
Sent: Friday, September 23, 2016 2:32 PM
To: histonet-at-lists.utsouthwestern.edu

X-from: joaoanatomia-at-gmail.com

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Email: joaoanatomia-at-gmail.com Name: Joao Paulo Rodrigues Marques

Organization: University of Sao Paulo

Title-Subject: [Filtered] CryoJane Slides - how to attach the sections to slides?

Message: I am trying to attach my sections from Leica Cryostat to the slides using CryoJane Tape
Transfer System (1x slides, tapes and UV light). My sections are good but when I stained the
slides my sections detaches and this is causing some problems because I need to wash my slides
several times (In situ hybridization and immunohistochemistry). What can I do to solve this
problem? How keep the sections attached to the slides? Does someone has experience with CryoJane
slides can give me more information?

Thanks and regards,


Joao Paulo R. Marques

Louisiana State University University of Sao Paulo

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From: wtivol-at-sbcglobal.net
Date: Sun, 25 Sep 2016 18:14:14 -0500
Subject: [Microscopy] Re: Best kind of bottles?

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} On Sep 21, 2016, at 1:43 PM, jkrupp-at-deltacollege.edu wrote:
}
} No laughing, I am curious to know what is the best kind of bottle/container for various EM stuff.
}
} I have always been a glass kind of guy. Thought plastic might have issues with some chemicals and I have had very old plastic bottles get brittle and go to pieces on the shelf.
}
} But, I am willing to reconsider. Plastic is lighter, maybe cheaper, and less breakable while young.
}
} Is plastic OK for most things. I’m thinking about storing stock solutions of buffers, acetone, alcohol, fixatives etc.
}
} I need to buy some new supplies and am wondering which way to go. Not exactly the paper or plastic question at the grocery store, but what are your thoughts?
}
} Jon
}
} Jonathan Krupp
} ASB&T
} San Joaquin Delta College
} 5151Pacific Ave.
} Stockton, CA 95207
} (209) 954-5284
} jkrupp-at-deltacollege.edu

Hi Jon,
It is not enough to specify “plastic”. Some plastics are softened or dissolved by acetone, for example. On the other hand, NaOH dissolves glass (over a long period of time), so the container should be selected for each specific reagent. Polyethylene and polypropylene are among the most stable plastics and should hold compatible reagents safely for a very long time, and they are unlikely to be damaged with even the roughest handling students will dish out. OsO4 can be problematic, but I think that polyethylene is OK for it—perhaps someone with more experience than I can correct me if I’m wrong. There are charts that list materials-reagents compatibility, and I’m sure they are available on the web. It’s these simple things that can play a role in maintaining lab safety, and you are to be commended for taking it seriously.
Yours,
Bill





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From: wim.hagen-at-me.com
Date: Mon, 26 Sep 2016 04:35:08 -0500
Subject: [Microscopy] Cryo-TEM specialist position at EMBL Heidelberg

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We have a fantastic job opportunity for a cryo-TEM specialist to help internal and external users with our Tecnai Polara, Talos Arctica and two Titan Krios microscopes.

http://www.embl.de/jobs/searchjobs/index.php?ref=HD_00969

Best regards,

Wim Hagen
EMBL Heidelberg

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From: microscopy.listserver-at-gmail.com
Date: Mon, 26 Sep 2016 17:40:49 -0500
Subject: [Microscopy] viaWWW: cryo-EM Postdoc Openings

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Title-Subject: [Filtered] 1 cryo-EM Postdoc Openings

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From: microscopy.listserver-at-gmail.com
Date: Mon, 26 Sep 2016 17:42:05 -0500
Subject: [Microscopy] viaWWW:MSNO 2016 Fall meeting

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Email: nxa157-at-case.edu Name: Nanthawan Avishai

Organization: Case Western Reserve University

Title-Subject: [Filtered] MSNO 2016 Fall meeting

Message: The Fall meeting of the Microscopy Society of NE Ohio (MSNO) will be held in the afternoon
on October 5 (Wednesday) from 3 pm at the modern ASM International Headquarters in Ohio, only 40 min
drive from here. You will have the opportunity to listen to two very interesting talks on:

1. SPR imaging analysis by Prof. Quan Jason Cheng from UC Riverside, CA
2. Microstructure-Property Relationships in Advanced Materials by Prof. John J. Lewandowski, CWRU

There will be a nice dinner between the lectures and the opportunity to connect with researchers and
students from other colleges and major US and international companies. The ASM International
Headquarters has an unique architectural design that is also worth seeing. All details for the
meeting can be found here:

http://www.msneo.org/2016-fall-meeting.html

If you are interested in microscopy and microanalysis but you are not a member yet, you are very
welcome to join now to enjoy the registration discount and more of our diverse programs (3
traditional meetings in winter, May, and fall, summer school, short courses, and more). The student
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Please register online at http://www.msneo.org/2016-fall-meeting-registration.html by Friday,
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From: microscopy.listserver-at-gmail.com
Date: Mon, 26 Sep 2016 17:42:59 -0500
Subject: [Microscopy] viaWWW:SCSAM Fall break 1-Day Short Course -Structural &

Contents Retrieved from Microscopy Listserver Archives
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Email: nxa157-at-case.edu Name: Nanthawan Avishai

Organization: Case Western Reserve University

Title-Subject: [Filtered] SCSAM Fall break 1-Day Short Course -Structural & Compositional Analysis
Solutions

Message: The Swagelok Center for Surface Analysis of Materials (SCSAM) is arranging a Fall Break
1-Day Short Course -Structural & Compositional Analysis Solutions on Monday - October 24 - White 411

Who should attend this course?
Graduate students, post-doctoral fellows, faculty, and anyone interested in microscopy, X-Ray
diffraction, and surface analysis.

Monday - October 24 - White 411
Morning Session 9:00-12:00PM

Dr. A. Avishai – Introduction

Dr. W. Jennings – Auger Electron Spectroscopy

Dr. K. Abbasi – X-Ray Photoelectron Spectroscopy/ESCA,
– Time of Flight Secondary Ion Mass Spectroscopy

Dr. J. Cowen – X-Ray Diffraction

Refreshments will be served at lunch break.

Afternoon Session 1:00-5:00PM

Ms. N. Avishai – Scanning Electron Microscopy

Dr. A. Avishai – Energy Dispersive Spectroscopy,

– Electron BackScattered Diffraction,

– Focused Ion Beam

Dr. D. Wang – Transmission Electron Microscopy and Electron Energy Loss Spectroscopy

Mr. R. Tomazin – Atomic Force Microscopy and Nanoindentation


The Swagelok Center for Surface Analysis of Materials provides:

* Access to high-end analytical equipment.

* Expert staff support. Access to vendor support.

* Training – equipment operation and data analysis.

* Tailoring solutions to be applied in research and industrial settings.

* Methods and expertise development.

The Swagelok Center for Surface Analysis of Materials is part of the larger CWRU Service Center
Group, serving as an extension of research and industrial lab infrastructures. Contact us to learn
how we can meet your needs or to discuss partnership opportunities.


Please register for the course by emailing Sharon Dingess at skd4-at-case.edu.

Use “2016 registration for SCSAM 1-Day Short Course” in the subject line.

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From: microscopy.listserver-at-gmail.com
Date: Thu, 29 Sep 2016 07:51:20 -0500
Subject: [Microscopy] viaWWW: EDS and 21 CFR part 11 Compliance

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Email: gtorraca-at-amgen.com Name: Gianpiero Torraca

Organization: Amgen

Title-Subject: [Filtered] Reaching out to ask about EDS and 21 CFR part 11 Compliance

Message: I’m writing you to figure out the industry landscape on SEM/EDS and part 11 compliance.
Please share your experience with getting the EDS in compliance and/or lack of success. It seems
slim pickings when it comes to EDS manufactures and compliance.
Gianni Torraca
Sr. Scientist, Amgen

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From: microscopy.listserver-at-gmail.com
Date: Thu, 29 Sep 2016 07:52:18 -0500
Subject: [Microscopy] viaWWW: Faculty/EM facility faculty director position available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: marie.cantino-at-uconn.edu

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Email: marie.cantino-at-uconn.edu Name: Marie Cantino

Organization: University of Connecticut, Biosciences Electron Microscopy Laboratory

Title-Subject: [Filtered] EM, Faculty/EM facility faculty director position available

Message: Job Posting Title: Faculty Position in Physiology and Neurobiology

The Department of Physiology and Neurobiology seeks applications from outstanding scientists for a
tenure-track position at the assistant, associate, or full professor level. The successful candidate
will be expected to maintain an independent research program addressing fundamentally important
questions in any area of Physiology and Neurobiology and serve as Faculty Director of the Bioscience
Electron Microscopy Laboratory (BEML; emlab.uconn.edu). Applicants must have a Ph.D. or equivalent
degree in Physiology, Neurobiology, Biophysics, Cell Biology, Structural Biology or related area;
significant post-degree research experience using Electron Microscopy; an outstanding scholarly
record including publications in leading journals; deep commitment to effective instruction at the
undergraduate and graduate levels; strong communication skills; and the ability to promote diversity
through their teaching and research programs.
The University of Connecticut (UConn) is in the midst of a transformational period of growth
supported by the $1.7B Next Generation Connecticut (http://nextgenct.uconn.edu/), the $1B Bioscience
Connecticut (http://health.uconn.edu/bioscience-ct/) investments, and a bold new Academic Plan: Path
to Excellence (https://issuu.com/uconnprovost/docs/academic-plan-single-hi-optimized_1).
Cutting-edge STEM research is further supported by the Center for Open Research Resources and
Equipment (http://core.uconn.edu/) , which oversees an exceptional collection of research support
facilities. We are pleased to continue these investments by inviting applications for this
tenure-track faculty position at the rank of Assistant Professor, one of two positions currently
open in the Department of Physiology and Neurobiology (http://www.pnb.uconn.edu/).

To Apply: Interested applicants must apply electronically (http://goo.gl/oEVzaT). Select “Apply” for
Search 2017070, Faculty Position in Physiology and Neurobiology. Please submit the following: a
cover letter; curriculum vitae; teaching statement; research and scholarship statement; commitment
to diversity statement; and contact information for five professional references. To ensure full
consideration, applications should be received no later than November 15, 2016. (Search 2017070).

For questions regarding this position, prospective applicants should contact
akiko.nishiyama-at-uconn.edu or joseph.loturco-at-uconn.edu, University of Connecticut, Department of
Physiology and Neurobiology, Unit 3156, 75 N. Eagleville Road, Storrs, CT 06269-3149.

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From: microscopy.listserver-at-gmail.com
Date: Thu, 29 Sep 2016 19:05:49 -0500
Subject: [Microscopy] viaWWW:Cupromeronic blue

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Email: gayle_schneider-at-urmc.rochester.edu Name: Gayle Schneider

Organization: University of Rochester

Title-Subject: [Filtered] Cupromeronic blue

Message: Does anyone have a supply of Cupromeronic blue? I have a researcher that wants to use it
to enhance proteoglycans in cornea for examination under TEM. I have been unsuccessful in locating
a company that supplies it. I will need 0.5g

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From: microscopy.listserver-at-gmail.com
Date: Thu, 29 Sep 2016 19:06:26 -0500
Subject: [Microscopy] viaWWW:Desktop carbon coater

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Email: roger.ristau-at-uconn.edu Name: Roger Ristau

Organization: University of Connecticut

Title-Subject: [Filtered] Desktop carbon coater

Message: We are upgrading from ancient bell-jar coaters to a new desktop carbon coater with TEM
sample prep in mind. Anyone who can advise on their experience with any brand coater is invited to
respond off line.

Cheers,

Roger Ristau
CAMMA
Univ of Connecticut


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From: microscopy.listserver-at-gmail.com
Date: Sat, 1 Oct 2016 07:40:48 -0500
Subject: [Microscopy] viaWWW: SEM Upgrades

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Email: mcolberg-at-mcl-inc.com Name: Mark Colberg

Title-Subject: [Filtered] SEM Upgrades

Message: I am looking for feedback from anyone who has an older model SEM upgraded with the Point
Electronic system. Any feedback, good or bad, would be helpful.
Thanks
-MRC

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From: wtivol-at-sbcglobal.net
Date: Sat, 1 Oct 2016 21:28:45 -0500
Subject: [Microscopy] Re: Re personal Re: Best kind of bottles?

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} On Sep 26, 2016, at 3:59 AM, MUSS Wolfgang (SCUR Board Member) {wij.muss-at-aon.at} wrote:
}
} Dear Bill:
} (Dear Jon),
}
} Thanks for your posting on "Best kind of bottles" which -as you stated -naturally always depends on "what" you are intending to "fill and store into.....".
} Most of your proposals I can second (e.g. NaOH as base in Polyethylene/Polypropylene bottles , vs. Acids in (Lab)Glass bottles.... but:
}
} I would like to comment first to you personally regarding OsO4 storage in polypropylene (generally in "Plastic") bottles:
}
} I have information ( as it also was my personal experience ) that any kind of plastic containers/bottles (polyethylene, polypropylene, polycarbonate, polystyrene/polystyrole, etc.) with regard to OsO4 (even recycled and therefore highly "pure" OsO2-precipitate) should be avoided.
}
} OsO4 solution/its vapors (also true for the OsO2 powder one might store after Reprocessing/recycling from used solutions) is highly reactive with "plastic" (which you and others for sure have seen as "black precipitate" inside a refrigerator if storage was not properly done.
}
} Proper storage of OsO4-solution IMHO only can be provided in a "Double-capped acid-flask/bottle (with grinded stopper/cap fits/seats)" made at least from (brown lab)glass as one can find in some glassware companies' catalogues.
}
} Even if you use such a flask you have to expect outgassing of the OsO4-vapor , so you might protect users as well as yourself in
} i) always using sufficient Parafilm- wrapping of both the stopper/sealing plug as well as the grinded outer glass cap,
} ii) placing the "Double-capped acid-flask/bottle" in a suitable fume cupboard / exhauster (if you store at room temperature for rapid use or short-dated applications), or better,
} ii) placing and freezing the "Double-capped acid-flask/bottle", wrapped by Parafilm in a separate refrigerator (at least at - 25°C - you can store in frozen state much more longer for later use or long-dated applications and just have to warm up to room temperature in a suitable fume cupboard / exhauster, which might take some 20-40 minutes without further stirring/vortexing)
}
} I am sorry and therefore apologize for lengthiness and Germanisms in my English writing style....If you would like to supplement your comment on OsO4-storage with what I brought to your attention, please feel free to use any of my words.
}
} Best wishes and regards,
} Wolfgang
}
} PS: any catalogue of plastic producing company/-ies at least in the last 10 years I found to contain valuable data on properties of diverse 'plastic brands' , for example:
} Google search for: " resistance of plastics to solvents " (more than 10.000,000 results), the first address being: http://www.plasticsintl.com/plastics_chemical_resistence_chart.html
}
} Wolfgang MUSS PhD/Dr. phil.
} Former Head of EM-Lab Pathology Inst. SALK-LKH and PMU (Paracelsus Medical Univ.) Salzburg
} PRIVATIER / private gentleman / Retiree
} since 1st of Dec. 2015
} PRIVATE ADRESS:
} Dr. phil. (PhD) Wolfgang (and Ingrid) Muß
} Ignaz-Rieder-Kai 19/6
} 5020 SALZBURG AUSTRIA/AUTRICHE/Österreich
} Mobile Phone: +43(0)676 5 369 456
} e-mail: wij.muss-at-aon.at
} MSA-Member (retired)
} Board Member of SCUR (until June 2017)[www.scur.org]
} Cf.: http://www.researchgate.net/profile/Wolfgang_MUSS
}
} The Society for Cutaneous Ultrastructure Research SCUR,
} The Skin Imaging Society at: www.scur.org
}
} Member of
} FB-ARMY of Electron Microscopists
} https://www.facebook.com/groups/2339731127/
} and
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}
}
}
} -----Ursprüngliche Nachricht-----
} Von: wtivol-at-sbcglobal.net [mailto:wtivol-at-sbcglobal.net]
} Gesendet: Montag, 26. September 2016 01:35
} An: wij.muss-at-aon.at
} Betreff: [Microscopy] Re: Best kind of bottles?
}
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}
} Hi Jon,
} It is not enough to specify "plastic". Some plastics are softened or dissolved by acetone, for example.
} On the other hand, NaOH dissolves glass (over a long period of time), so the container should be selected for each specific reagent.
} Polyethylene and polypropylene are among the most stable plastics and should hold compatible reagents safely for a very long time, and they are unlikely to be damaged with even the roughest handling students will dish out.
} OsO4 can be problematic, but I think that polyethylene is OK for it—perhaps someone with more experience than I can correct me if I’m wrong.
} There are charts that list materials-reagents compatibility, and I’m sure they are available on the web. It’s these simple things that can play a role in maintaining lab safety, and you are to be commended for taking it seriously.
} Yours,
} Bill
}
}
}
} } On Sep 21, 2016, at 1:43 PM, jkrupp-at-deltacollege.edu wrote:
} } No laughing, I am curious to know what is the best kind of bottle/container for various EM stuff.
} } I have always been a glass kind of guy. Thought plastic might have issues with some chemicals and I have had very old plastic bottles get brittle and go to pieces on the shelf.
} } But, I am willing to reconsider. Plastic is lighter, maybe cheaper, and less breakable while young.
} } Is plastic OK for most things. I’m thinking about storing stock solutions of buffers, acetone, alcohol, fixatives etc.
} } I need to buy some new supplies and am wondering which way to go. Not exactly the paper or plastic question at the grocery store, but what are your thoughts?
} } Jon
}
} } Jonathan Krupp
} } ASB&T
} } San Joaquin Delta College
} } 5151Pacific Ave.
} } Stockton, CA 95207
} } (209) 954-5284
} } jkrupp-at-deltacollege.edu

Dear Wolfgang,
I second your advice about OsO4. As I think about it, even PE or PP could have plasticizer with double bonds, and Os black ppt could end up in micro-cracks that develop as a result of reaction with OsO4.
Yours,
Bill





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From: les-at-zsgenetics.com
Date: Sun, 2 Oct 2016 07:14:40 -0500
Subject: [Microscopy] Short-term TEM operator need in Boston area

Contents Retrieved from Microscopy Listserver Archives
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Greetings,
Our Boston- area company is in immediate need of a Tecnai TEM
operator to help cover demand on a current project. If you are in
the area and interested, feel free to contact me via the email below.

-Larry Scipioni
les-at-zsgenetics-dot-com
ZS Genetics


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From: dcristofori-at-unive.it
Date: Mon, 3 Oct 2016 12:41:00 -0500
Subject: [Microscopy] Rotary pump on Jeol TEM

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers,
we have a small problem with the rotary pump serving our Jeol JEM-3010.
Given the age of the pump, we are evaluating whether try to fix the
problem or buy a new pump. In the latter case, we would prefer to buy a
different brand than the original one provided with the TEM. But we're
not sure about the compatibility of the pumps when dealing with the
control signal used by the TEM electronics to operate the rotary pump.
Does anyone have experience with such an issue?
Any contribution will be greatly appreciated.

Davide


~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~
Davide Cristofori

direct phone = 041.234.67.26
e-mail = dcristofori[at]unive[dot]it

Centro di Microscopia Elettronica "Giovanni Stevanato" e
Dipartimento di Sc. Molecolari e Nanosistemi
Universit Ca' Foscari Venezia

Campus Scientifico, Edificio ETA
Via Torino 155 I-30172 Mestre (VE) Italy
~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~

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From: drk-at-shcc.org
Date: Mon, 3 Oct 2016 16:00:16 -0500
Subject: [Microscopy] Register for the Pacific NW Microscopy Symposium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings Fellow Microscopists,

The Pacific Northwest Microscopy Society will hold its 2016 Fall symposium on Thursday November 3rd, continuing Friday November 4th. The program will showcase innovative area scientists pioneering the latest technologies in microscopy. Both invited and contributed talks will be presented at the Portland Shriners Hospital for Children. Workshops will be conducted at the nearby Center for Spatial Systems Biology at the new Oregon Health Sciences University waterfront campus. Here is an opportunity to interact with those at the forefront of emerging technologies in a friendly atmosphere with many opportunities for conversation and collaboration. We encourage you to present your work in the Friday afternoon session! The preliminary program and registration can be found on the Pacific Northwest Microscopy Society webpage at:

http://pacificnwmicroscopy.org

Please plan to attend this informative program and meet with your friends, colleagues and vendors.

Thank you,

Doug

Douglas R. Keene
President, Pacific NW Microscopy Society
Micro-Imaging Center
Shriners Hospital for Children
3101 SW Sam Jackson Park Road
Portland, Oregon 97239
Office: 503-221-3434
Mobile: 503-819-3600



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From: amit.welcomes.u-at-gmail.com
Date: Tue, 4 Oct 2016 00:02:43 -0500
Subject: [Microscopy] Sudden flashes seen on Fluorescent screen of TEM

Contents Retrieved from Microscopy Listserver Archives
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Hi,
we are using JEOL 2100F electron microscope. From past 2-3 days we can
see a sudden "flash" of light on flourescent screen along with a faint
"click" sound. Rest of tem works as normal. This id more prevalent if
I meddle too much with brightness knob.
Can anyone suggest a reason for this?

==============================Original Headers==============================
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1, 37 -- Subject: Sudden flashes seen on Fluorescent screen of TEM
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From: Nicolas.Stephant-at-univ-nantes.fr
Date: Tue, 4 Oct 2016 03:20:35 -0500
Subject: [Microscopy] Re: Sudden flashes seen on Fluorescent screen of TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,
I believe you have seen an electric discharge. May be the connection
between the screen and the ground is pretty bad. This connection is done
through a spring under the screen and the circuit for measuring the
current received by the screen.

Nicolas STEPHANT

Universit de Nantes
Institut Jean Rouxel
Service de microscopie lectronique balayage et microanalyse
2 rue de la Houssinire
BP 92208
44322 Nantes cdex 3

Tl : 02 40 37 64 26
Ml : nicolas.stephant-at-univ-nantes.fr
Web : http://cnrs-imn.fr

"Le monde n'existe que pour autant que nous sommes capables d'en produire une image"
C.G Jung

Le 04/10/2016 07:16, amit.welcomes.u-at-gmail.com a crit :
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} Hi,
} we are using JEOL 2100F electron microscope. From past 2-3 days we can
} see a sudden "flash" of light on flourescent screen along with a faint
} "click" sound. Rest of tem works as normal. This id more prevalent if
} I meddle too much with brightness knob.
} Can anyone suggest a reason for this?
}
} ==============================Original Headers==============================
} 1, 37 -- From amit.welcomes.u-at-gmail.com Tue Oct 4 00:02:43 2016
} 1, 37 -- Received: from mail-yb0-f194.google.com (mail-yb0-f194.google.com [209.85.213.194])
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} 1, 37 -- Message-ID: {CA+bx8_wx4DnW7P-d4AEAuty0r191PHd12-yH=8JcZcdNwGfU-w-at-mail.gmail.com}
} 1, 37 -- Subject: Sudden flashes seen on Fluorescent screen of TEM
} 1, 37 -- To: microscopy {microscopy-at-microscopy.com}
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==============================Original Headers==============================
7, 26 -- From Nicolas.Stephant-at-univ-nantes.fr Tue Oct 4 03:20:34 2016
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7, 26 -- Reply-To: Nicolas.Stephant-at-univ-nantes.fr
7, 26 -- Subject: Re: [Microscopy] Sudden flashes seen on Fluorescent screen of TEM
7, 26 -- References: {201610040516.u945Gb8X017979-at-ns.microscopy.com}
7, 26 -- To: amit.welcomes.u-at-gmail.com, microscopy-at-microscopy.com
7, 26 -- From: Nicolas Stephant {Nicolas.Stephant-at-univ-nantes.fr}
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From: Nicolas.Stephant-at-univ-nantes.fr
Date: Tue, 4 Oct 2016 03:54:14 -0500
Subject: [Microscopy] Re: Rotary pump on Jeol TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Davide,
This pump operates with 200 V or 100 V (see instructions on the plate).
It's easy now to find pump that accept large range of voltage. You will
have to connect this new pump through the original JEOL connector by
recovering the plug from the old pump and reconnecting to the new one
according to the JEOL diagrams. That way the TEM will command the pump
as before.
What is more difficult is to disabled the belt breaking security. The
vacuum circuit of the microscope received permanently a signal modulated
by the rotation of the pulley of the pump to ensure that the pump is
working. Simply disconnecting the cable towards the sensor is not
enought. One pin of one of the integrated circuit of the vacuum PB must
be removed (lift up), however, with a potential danger for the
microscope if the pump stops. I can not answer more precisely without
diagrams.

Nicolas STEPHANT

Universit de Nantes
Institut Jean Rouxel
Service de microscopie lectronique balayage et microanalyse
2 rue de la Houssinire
BP 92208
44322 Nantes cdex 3

Tl : 02 40 37 64 26
Ml : nicolas.stephant-at-univ-nantes.fr
Web : http://cnrs-imn.fr

"Le monde n'existe que pour autant que nous sommes capables d'en produire une image"
C.G Jung

Le 03/10/2016 19:57, dcristofori-at-unive.it a crit :
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} Dear Listers,
} we have a small problem with the rotary pump serving our Jeol JEM-3010.
} Given the age of the pump, we are evaluating whether try to fix the
} problem or buy a new pump. In the latter case, we would prefer to buy a
} different brand than the original one provided with the TEM. But we're
} not sure about the compatibility of the pumps when dealing with the
} control signal used by the TEM electronics to operate the rotary pump.
} Does anyone have experience with such an issue?
} Any contribution will be greatly appreciated.
}
} Davide
}
}
} ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~
} Davide Cristofori
}
} direct phone = 041.234.67.26
} e-mail = dcristofori[at]unive[dot]it
}
} Centro di Microscopia Elettronica "Giovanni Stevanato" e
} Dipartimento di Sc. Molecolari e Nanosistemi
} Universit Ca' Foscari Venezia
}
} Campus Scientifico, Edificio ETA
} Via Torino 155 I-30172 Mestre (VE) Italy
} ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~
}
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} 7, 46 -- From: Davide Cristofori {dcristofori-at-unive.it}
} 7, 46 -- Subject: Rotary pump on Jeol TEM
} 7, 46 -- To: microscopy-at-microscopy.com
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7, 26 -- Subject: Re: [Microscopy] Rotary pump on Jeol TEM
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From: amit.welcomes.u-at-gmail.com
Date: Tue, 4 Oct 2016 04:33:04 -0500
Subject: [Microscopy] Re: Sudden flashes seen on Fluorescent screen of TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

On Tue, Oct 4, 2016 at 1:52 PM, Nicolas Stephant
{Nicolas.Stephant-at-univ-nantes.fr} wrote:
} Hi,
} I believe you have seen an electric discharge. May be the connection between
} the screen and the ground is pretty bad. This connection is done through a
} spring under the screen and the circuit for measuring the current received
} by the screen.
}
} Nicolas STEPHANT
}

} Hello
}
} it might be a contamination in the Wehnelt cylinder or equivalent other electron soure
}
} Jan


Is there any outlet where say I can measure and demonstrate the above?
i.e say any output like say ground wire, where i can safely connect a
multimeter and check?
Also If it's wehnelt contamination etc, can it be remidified without
calling engineers? As maintanence contract is not there I would like
to provide rough estimate to administration before calling jeol for
help

==============================Original Headers==============================
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4, 40 -- Date: Tue, 4 Oct 2016 15:04:36 +0530
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4, 40 -- Subject: Re: [Microscopy] Sudden flashes seen on Fluorescent screen of TEM
4, 40 -- To: Nicolas.Stephant-at-univ-nantes.fr, microscopy {microscopy-at-microscopy.com}
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From: FMonson-at-wcupa.edu
Date: Tue, 4 Oct 2016 14:13:43 -0500
Subject: [Microscopy] Re: Sudden flashes seen on Fluorescent screen of TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

If you have arcing in the gun, you should get a Geiger counter and check for radiation. If your image shakes, rattles or rolls, then I might go with the gun.

I agree, however, with the screen posibility.
You may see arcs, but do you see the beam move? Or 'flutter' on the screen. Even worse, at high mag, is your focus unstable. If just flashes of light, but not the other phenomena, You might want to vent the camera - viewing area - and check under the screen.

NOTE: Radio Shack has a glass fiber 'pen' that is designed to clean contact surfaces. If the spring is attached to ground, you might save your bosses a visit by cleaning the contact area on the bottom of the screen.

NOTE: I have a rule about electron beams. When they misbehave, I always change the filament first. If there is no change, then, depending on age, I might save the filament.

NOTE: Sometime, the gun housing just needs a good cleaning, but with my FEI/Philips Tecnai, there follows a lot of outgasing even after using the Sun lamp to start the drying out.

Hope some of this helps,

Fred Monson

Frederick C Monson, PhD
Center for Microanalysis and Imaging, Research and Training (CMIRT)
West Chester University of Pannsylvania
West Chester, PA, 1938
610-738-0437
fmonson-at-wcupa.edu
________________________________________
X-from: amit.welcomes.u-at-gmail.com {amit.welcomes.u-at-gmail.com}
Sent: Tuesday, October 4, 2016 5:44 AM
To: Monson, Frederick

On Tue, Oct 4, 2016 at 1:52 PM, Nicolas Stephant
{Nicolas.Stephant-at-univ-nantes.fr} wrote:
} Hi,
} I believe you have seen an electric discharge. May be the connection between
} the screen and the ground is pretty bad. This connection is done through a
} spring under the screen and the circuit for measuring the current received
} by the screen.
}
} Nicolas STEPHANT
}

} Hello
}
} it might be a contamination in the Wehnelt cylinder or equivalent other electron soure
}
} Jan


Is there any outlet where say I can measure and demonstrate the above?
i.e say any output like say ground wire, where i can safely connect a
multimeter and check?
Also If it's wehnelt contamination etc, can it be remidified without
calling engineers? As maintanence contract is not there I would like
to provide rough estimate to administration before calling jeol for
help

==============================Original Headers==============================
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From: microwink-at-gmail.com
Date: Tue, 4 Oct 2016 20:55:52 -0500
Subject: [Microscopy] Sudden flashes seen on Fluorescent screen of TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Amit,

Since the 2100F is a FEG system, the gun components differ than those
described by some of the responses. I would avoid opening the FEG
unless directed by service, or if you decide to replace the FEG tip.
Replacement of FEG is not trivial (nor cheap), unlike W or LaB6, so
this is not where I would start...

The click you are hearing is worrisome. Is it coming from the gun area
(top of microscope)? Or perhaps from the HT tank behind the
microscope? Try to determine whether the FEG emission current is
fluctuating shortly before or after you hear this click. You may need
to set up a camera or screen capture software to monitor the emission
current (and vacuum levels!).

If the click is coming from the gun area, this is usually indicative
of arcing. Bad! Usually caused by lint/dust/particulate contamination
in the gun region. This would be unusual for a FEG. Has the FEG been
opened in the recent past? If the noise is coming from the HT tank,
then you may be experiencing a problem with the tank, which would
necessitate a service call.

Since this is the 2100 series, it must be relatively young, correct?
Probably not an electronics issue, so I'm not sure why changing C2/C3
(brightness) knob would affect operation.

As other have indicated, it could be related to charging of the InP
screens (don't forget little screen). But click noise means, in my
opinion, more sinister problem that you need to find. I hope not!

Good luck,
Chris

On Tue, Oct 4, 2016 at 5:43 AM, {amit.welcomes.u-at-gmail.com} wrote:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
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}
} On Tue, Oct 4, 2016 at 1:52 PM, Nicolas Stephant
} {Nicolas.Stephant-at-univ-nantes.fr} wrote:
} } Hi,
} } I believe you have seen an electric discharge. May be the connection between
} } the screen and the ground is pretty bad. This connection is done through a
} } spring under the screen and the circuit for measuring the current received
} } by the screen.
} }
} } Nicolas STEPHANT
} }
}
} } Hello
} }
} } it might be a contamination in the Wehnelt cylinder or equivalent other electron soure
} }
} } Jan
}
}
} Is there any outlet where say I can measure and demonstrate the above?
} i.e say any output like say ground wire, where i can safely connect a
} multimeter and check?
} Also If it's wehnelt contamination etc, can it be remidified without
} calling engineers? As maintanence contract is not there I would like
} to provide rough estimate to administration before calling jeol for
} help
}
} ==============================Original Headers==============================
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8, 38 -- Subject: Re: [Microscopy] Re: Sudden flashes seen on Fluorescent screen of TEM
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From: amit.welcomes.u-at-gmail.com
Date: Wed, 5 Oct 2016 01:03:33 -0500
Subject: [Microscopy] Re: Sudden flashes seen on Fluorescent screen of TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Sorry for the delayed reply,

It is a field emission gun as many noted hence no question of filaments etc.

} } If you have arcing in the gun, you should get a Geiger counter and check for radiation. If your image } } shakes, rattles or rolls, then I might go with the gun.


This looks simple enough! Though just curious, almost all enclosures
in TEM are heavily shielded with lead right? If there are any flashes
in gun, how can Geiger counter detect it? I mean will any stray
radiation make its way out?

Also I called one of engineer today they said in case of any arcing
etc in gun, emission is most likely to go off (I desperately want it
to be not gun fault!)


} } I agree, however, with the screen posibility.
} } You may see arcs, but do you see the beam move? Or 'flutter' on the screen. Even worse, at high mag, is } } your focus unstable. If just flashes of light, but not the other phenomena, You might want to vent the camera } } ?} } viewing area - and check under the screen.

As such there is no impact on image, but I will carry out more
thorough observation today to see.


} } One more idea. Maybe the click you hear is an electronics relay
} } opening and closing because of electronics malfunction. This is the
} } best case. Should be easy to find with a set of good ears,
} } stethoscope, or helpful review of the wiring diagrams showing control
} } panel (subsystem) circuits.

Malfunctioning relay does not occur to me. I will try it.


} } The click you are hearing is worrisome. Is it coming from the gun area
} } (top of microscope)? Or perhaps from the HT tank behind the
} } microscope? Try to determine whether the FEG emission current is
} } fluctuating shortly before or after you hear this click. You may need
} } to set up a camera or screen capture software to monitor the emission
} } current (and vacuum levels!).

JEOL software does provide an on screen reading of current of
electrons falling on screen, as such I could not detect any
fluctuations in them. If arcing is in gun, then JEOL engineers told
that it will shut down the emission.


} } If the click is coming from the gun area, this is usually indicative
} } of arcing. Bad! Usually caused by lint/dust/particulate contamination
} } n the gun region. This would be unusual for a FEG. Has the FEG been
} } opened in the recent past? If the noise is coming from the HT tank,
} } then you may be experiencing a problem with the tank, which would
} } necessitate a service call.

No. FEG was not opened any time in past few years.


} } Since this is the 2100 series, it must be relatively young, correct?
} } Probably not an electronics issue, so I'm not sure why changing C2/C3
} } (brightness) knob would affect operation.

Commissioned in 2011. I am not entirely sure of correlation with C2/C3
as well, but if it is charging of screen in such case increasing
brightness will increase chanses of discharge. Would it not?


} } As other have indicated, it could be related to charging of the InP
} } screens (don't forget little screen). But click noise means, in my
} } opinion, more sinister problem that you need to find. I hope not!

} } Good luck,
} } Chris



} } Since it changes with screen brightness it is most likely charging of the
} } viewing glass. Has someone had the viewing glass out recently for some
} } reason?

No. operating column, including screen chamber has not been opened in
past 3-4 years as per my knowledge/


} } The viewing glass has a conductive coating on the vacuum side to dissipate
} } any charge that may build up from the beam. A small spring touches the glass
} } in the lower left corner providing a path to ground for any charge.
} } If the glass is reversed or the spring is missing you will experience the
} } phenomena you describe.
} } To check the glass - use an ohm meter to check the continuity of the glass
} } on the atmosphere side. Just touch both leads anywhere on the glass. If you
} } have continuity then the glass is backwards and needs to be reversed. If it
} } shows open (no continuity) then look to see if the spring is visible in the
} } lower left corner of the glass.

I will check for the spring. Also like chris suggested above for
checking discharges with Geiger counter, would discharges in screen
chamber will also show up on Geiger counter?


} } Regards,
} } Joe



Thank you everyone for help, I will keep posted


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From: oshel1pe-at-cmich.edu
Date: Wed, 5 Oct 2016 07:22:14 -0500
Subject: [Microscopy] Re: Sudden flashes seen on Fluorescent screen of TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Besides the suggestions you have already received, when was the last time the gun and column were baked? The clicking and fluctuating condensers make me wonder if there is a bit of contamination on the condenser aperture. Baking might take care of that.

Did this problem start after a particular sample was imaged? One that might cause contamination?

Phil

Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576
________________________________________
X-from: amit.welcomes.u-at-gmail.com {amit.welcomes.u-at-gmail.com}
Sent: Wednesday, October 5, 2016 02:19
To: Oshel, Philip Eugene

Sorry for the delayed reply,

It is a field emission gun as many noted hence no question of filaments etc.

} } If you have arcing in the gun, you should get a Geiger counter and check for radiation. If your image } } shakes, rattles or rolls, then I might go with the gun.


This looks simple enough! Though just curious, almost all enclosures
in TEM are heavily shielded with lead right? If there are any flashes
in gun, how can Geiger counter detect it? I mean will any stray
radiation make its way out?

Also I called one of engineer today they said in case of any arcing
etc in gun, emission is most likely to go off (I desperately want it
to be not gun fault!)


} } I agree, however, with the screen posibility.
} } You may see arcs, but do you see the beam move? Or 'flutter' on the screen. Even worse, at high mag, is } } your focus unstable. If just flashes of light, but not the other phenomena, You might want to vent the camera } } ?} } viewing area - and check under the screen.

As such there is no impact on image, but I will carry out more
thorough observation today to see.


} } One more idea. Maybe the click you hear is an electronics relay
} } opening and closing because of electronics malfunction. This is the
} } best case. Should be easy to find with a set of good ears,
} } stethoscope, or helpful review of the wiring diagrams showing control
} } panel (subsystem) circuits.

Malfunctioning relay does not occur to me. I will try it.


} } The click you are hearing is worrisome. Is it coming from the gun area
} } (top of microscope)? Or perhaps from the HT tank behind the
} } microscope? Try to determine whether the FEG emission current is
} } fluctuating shortly before or after you hear this click. You may need
} } to set up a camera or screen capture software to monitor the emission
} } current (and vacuum levels!).

JEOL software does provide an on screen reading of current of
electrons falling on screen, as such I could not detect any
fluctuations in them. If arcing is in gun, then JEOL engineers told
that it will shut down the emission.


} } If the click is coming from the gun area, this is usually indicative
} } of arcing. Bad! Usually caused by lint/dust/particulate contamination
} } n the gun region. This would be unusual for a FEG. Has the FEG been
} } opened in the recent past? If the noise is coming from the HT tank,
} } then you may be experiencing a problem with the tank, which would
} } necessitate a service call.

No. FEG was not opened any time in past few years.


} } Since this is the 2100 series, it must be relatively young, correct?
} } Probably not an electronics issue, so I'm not sure why changing C2/C3
} } (brightness) knob would affect operation.

Commissioned in 2011. I am not entirely sure of correlation with C2/C3
as well, but if it is charging of screen in such case increasing
brightness will increase chanses of discharge. Would it not?


} } As other have indicated, it could be related to charging of the InP
} } screens (don't forget little screen). But click noise means, in my
} } opinion, more sinister problem that you need to find. I hope not!

} } Good luck,
} } Chris



} } Since it changes with screen brightness it is most likely charging of the
} } viewing glass. Has someone had the viewing glass out recently for some
} } reason?

No. operating column, including screen chamber has not been opened in
past 3-4 years as per my knowledge/


} } The viewing glass has a conductive coating on the vacuum side to dissipate
} } any charge that may build up from the beam. A small spring touches the glass
} } in the lower left corner providing a path to ground for any charge.
} } If the glass is reversed or the spring is missing you will experience the
} } phenomena you describe.
} } To check the glass - use an ohm meter to check the continuity of the glass
} } on the atmosphere side. Just touch both leads anywhere on the glass. If you
} } have continuity then the glass is backwards and needs to be reversed. If it
} } shows open (no continuity) then look to see if the spring is visible in the
} } lower left corner of the glass.

I will check for the spring. Also like chris suggested above for
checking discharges with Geiger counter, would discharges in screen
chamber will also show up on Geiger counter?


} } Regards,
} } Joe



Thank you everyone for help, I will keep posted


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From: microscopy.listserver-at-gmail.com
Date: Thu, 6 Oct 2016 18:17:49 -0500
Subject: [Microscopy] viaWWW:cost of replacing a FEG emitter

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Email: alfredotolley-at-hotmail.com Name: Alfredo Tolley

Organization: Metals Physics Division Atomic Energy Commission of Argentina

Title-Subject: [Filtered] cost of replacing a FEG emitter

Message: Hello,

I am trying to find out what is the labor cost for replacing a FEG emitter for a 200 keV TEM.

We have the spare part, but would like to know what is the labor cost for replacing the used emitter.
Thanks!

Alfredo




Alfredo Tolley

Physics of Metales Divisin

Centro Atmico Bariloche

Bariloche - Argentina

Ph: 54 294 444 5268

alfredotolley-at-hotmail.com


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From: microscopy.listserver-at-gmail.com
Date: Thu, 6 Oct 2016 18:18:29 -0500
Subject: [Microscopy] viaWWW:FIB Technician Opening

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Email: ming_j_zhang-at-amat.com Name: Ming Zhang

Organization: Applied Materials Company

Title-Subject: [Filtered] FIB Technician Opening

Message: Applied Materials Company has an immediate opening for an FIB technician in Portland, Oregon.

Applied Materials Company is the world's leading semiconductor equipment manufacturing company and
one of top 500 U.S. Companies.

The successful candidate will cover night shift and mainly focus on TEM sample preparation.

An associate degree or equivalent in Engineering, Materials Science, Chemistry, or Physics, etc. is
required.
US citizen or green card holder preferred.

Interested individuals should send their resumes to Drew_Goettler-at-amat.com

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From: rjpalmer-at-dir.nidcr.nih.gov
Date: Fri, 7 Oct 2016 08:12:49 -0500
Subject: [Microscopy] Technovit vs MMA

Contents Retrieved from Microscopy Listserver Archives
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Hello all -

I am having a discussion with a colleague about approaches to
thin-sectioning teeth (i.e., enamel). Historically, this has been done
with decalcification, embedding in MMA resins, then sectioning - meaning
months from start to finish. Recently, people have used a
cold-polymerizing GMA resin with the commercial name Technovit 8100.
Teeth are embedded, cut into preparatory sections (1 mm) with a saw,
decalcified, then re-embedded and thin-sectioned on an ultramicrotome
using tungsten (and even glass) blades. That process start-to-finish
takes a month (simply because of the EDTA decalcification). My colleague,
who is a very a talented bone histologist, just says flat out that
Technovit is a waste of time because it is GMA and just too soft. Im
wondering whether anyone here has first-hand experience with Technovit and
would care to comment on its advantages/disadvantages compared to MMA.
Specifically, Id love to hear something about relative hardness. In
the end, we want to do in-situ hybridization and fluorescence microscopy.

Thanks in advance for your perspectives!

Robert J. Palmer Jr., PhD
National Institute of Dental and Craniofacial Research
National Institutes of Health
Building 30, Room 331
Bethesda MD 20892
301-594-0025




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From: microscopy.listserver-at-gmail.com
Date: Mon, 10 Oct 2016 07:31:04 -0500
Subject: [Microscopy] viaWWW: Postdoctoral Opportunity: Environmental RSoXS

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Email: brian.collins-at-wsu.edu Name: Brian Collins

Organization: Washington State University

Title-Subject: [Filtered] Postdoctoral Opportunity: Environmental RSoXS

Message: A Postdoctoral Research Fellow position is available with professor Brian A. Collins, in
the Department of Physics at Washington State University (WSU) in partnership with Lawrence Berkeley
National Laboratory (LBNL).

Nano-to-mesoscale interactions and structures within organic systems are of critical importance for
our understanding of their macroscopic properties and functions, many of which only occur in
specific operational environments. Resonant soft X-ray scattering (RSoXS) is uniquely sensitive to
bond-type, bond-orientation, and is capable of probing these materials with high sensitivity using
their intrinsic chemical structure rather than laborious or disruptive labeling. Currently, however,
RSoXS can only be performed on samples suspended in vacuum. The development of environmental control
(gases, liquids, temperatures, electric fields, and charges) will allow leaps in our knowledge of
interactions and structures of organic materials that are uniquely relevant to the very applications
in which they form, exist and function.
DUTIES: The successful candidate is expected to develop a new instrument at beamline 11.0.1.2 that
incorporates environmental control and stimuli during RSoXS measurements on soft matter systems
enabling in-situ and in-operando measurements on materials applicable to organic devices or
biological systems. This will involve a new chamber, detector, and a commercial flow apparatus
commonly used for transmission electron microscopes (TEMs). The work is expected to involve
incorporation of systems, design and lithographic fabrication of environmental ‘cells’, and
demonstration of the final instrument by applying its capabilities in a scientific project that
combines TEM and RSoXS. With RSoXS beamlines being developed at synchrotrons around the world, this
work will demonstrate leadership and expertise in a widely expanding field with excellent career
opportunities.

REQUIREMENTS: Candidates must have significant experience in either synchrotron X-ray
scattering/spectroscopy techniques or in-situ flow-cell experiments in a TEM. Computer program
experience is highly encouraged. Candidates must also have a Ph.D. in Physics, Chemistry,
Chemical/Materials Engineering, or a related field.

APPOINTMENT: This is a joint appointment between WSU and the LBNL Postdoctoral Fellowship Program
with benefits associated with WSU. The salary is $61,200/yr with appropriate experience. The term is
for one year, with the possibility of renewal based on satisfactory performance. A few month’s prep
work locally at WSU is preferred with the remainder of the appointment to occur at LBNL. Preferred
start date is as soon as available.

HOW TO APPLY: Interested applicants should submit a cover letter, CV (including list of
publications), and arrange to have three letters of recommendation sent to Brian A. Collins at
brian.collins-at-wsu.edu. Review of applications will begin immediately. The position will remain open
until filled. WSU is an EO/AA Educator and Employer.


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From: microscopy.listserver-at-gmail.com
Date: Mon, 10 Oct 2016 17:21:39 -0500
Subject: [Microscopy] viaWWW:Research Associate Position in DC

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Email: chrisbrantner-at-gwu.edu Name: Chris Brantner

Organization: George Wasahington University

Title-Subject: [Filtered] Research Associate Position in DC

Message: https://www.gwu.jobs/postings/38256

Greetings Microscopists,

I have a position open in my lab for a research associate at the George Washington University in the
new Science and Engineering Hall in the Nanofabrication and Imaging Center. If you can prepare
samples for EM and want to work in an exciting new lab, then check out the link to the description.

Chris Brantner
Senior Research Scientist in Electron Microscopy
George Washington University
Washington DC

chrisbrantner-at-gwu.edu

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From: microscopy.listserver-at-gmail.com
Date: Wed, 12 Oct 2016 20:39:22 -0500
Subject: [Microscopy] viaWWW:remote imaging on Hitachi S4700

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Email: wambrose-at-unc.edu Name: Wallace Ambrose

Organization: UNC

Title-Subject: [Filtered] remote imaging on Hitachi S4700

Message: We have previously instituted remote imaging on our FEI Quanta 200 for broadcasting live
demos to our local school area. We also wanted to do the same with our Hitachi S4700. Although we
can load the server/client software (TightVNC) and get a connection, the image does not port to the
client pc. We get a "purple screen". Has anyone been able to get the live (or captured) image from
the Hitachi onto a network client?
Thanks
Wallace

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From: nmz787-at-gmail.com
Date: Thu, 13 Oct 2016 00:10:53 -0500
Subject: [Microscopy] Re: viaWWW:remote imaging on Hitachi S4700

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Wallace,
I believe you're seeing the effects of hardware-acceleration with the
video. What I am guessing is happening is the microscope image is
transferred via DMA (direct memory access) to the video card directly,
bypassing the operating system. VNC however grabs the video frame
buffer from the operating system, which doesn't have any information
(because after that image is transported to the video card, the DMA
transaction fills in the purple section before being sent out to the
monitor).

What I did for an older 1990s FEI FIB 200, was to use a VGA video
cable splitter:
http://www.siig.com/av-products/splitters-distribution-amplifiers/vga/1x2-compact-vga-splitter.html
and then a VGA2USB frame grabber:
https://www.epiphan.com/products/vga2usb/

Then get another computer, an old desktop or laptop or even a new
mini-PC like an Intel NUC or compute-stick or RasperryPi ( {=$40),
which can stream/save the video however you like.

You'll just need to get a splitter and grabber for your specific video
cable (I'm guessing VGA or DVI):
https://www.epiphan.com/products/#usb-video-grabbers

-Nathan



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} Title-Subject: [Filtered] remote imaging on Hitachi S4700
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} demos to our local school area. We also wanted to do the same with our Hitachi S4700. Although we
} can load the server/client software (TightVNC) and get a connection, the image does not port to the
} client pc. We get a "purple screen". Has anyone been able to get the live (or captured) image from
} the Hitachi onto a network client?
} Thanks
} Wallace
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--
-Nathan

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From: nicholls-at-uic.edu
Date: Thu, 13 Oct 2016 08:26:05 -0500
Subject: [Microscopy] XEDS: Problem with peak splitting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues,

We have a problem with an older Noran Si(Li) detector running on a Voyager
platform (it is about 19 years old!) that I have not come across before.

Suddenly this week instead of getting a single peak, all of the peaks in
the spectrum are split into doublets. For instance for Al K at 1.486keV we
get two peaks separated by ~100eV and centered at 1.486keV.

Has anybody seen this and is there a cure?

Alan


--
Alan W Nicholls, PhD
Associate Director, RRC
Director Research Resource Facility - Electron Microscopy
Research Resources Center, University of Illinois at Chicago
Rm 110, 845 West Taylor St
Chicago, IL 60607
312 996 1227
nicholls-at-uic.edu


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From: tbargar-at-unmc.edu
Date: Thu, 13 Oct 2016 10:12:59 -0500
Subject: [Microscopy] like to hear from users of the K-Kit

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We've recently bought a K-Kit starter set from EMS. The K-Kit allows the viewing of wet samples in the TEM. I would like to hear from anyone else out there who has used it to get your thoughts and experiences from using it. Any and all information would be welcomed, as usual please respond privately.

Tom Bargar
UNMC
Electron Microscopy Core Facility
986395 Nebraska Medical Center
Omaha, NE 68198-6395
402-559-7347
tbargar-at-unmc.edu


The information in this e-mail may be privileged and confidential, intended only for the use of the addressee(s) above. Any unauthorized use or disclosure of this information is prohibited. If you have received this e-mail by mistake, please delete it and immediately contact the sender.



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From: amenex-at-amenex.com
Date: Thu, 13 Oct 2016 11:52:13 -0500
Subject: [Microscopy] Re: XEDS: Problem with peak splitting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Dr.Nicholls -

Sixteen years out from any contact with Amenex's ETEC Autoprobe, I
recall
seeing line doubling when the detector was getting overloaded,
especially
in my hands. The context is that I was trying to write code in TrueBasic
to automate the process of setting the wavelength-dispersive
spectrometers
at the correct angle to detect the X-rays coming from specific elements,
whose wavelengths are ever so slightly dependent on their chemical
surroundings. The software was to scan across the selected wavelength
and
then select the angle at which the signal was at its maximum. Whenever
the detector gain was excessive, the peak would get folded over onto
itself, producing the false doublet.

Just back off the gain setting.

Hope this helps.

George Langford, Sc.D.
Amenex Associates, Inc.
http://www.amenex.com/
amenex-at-amenex.com
You wrote:
} ----------------------------------------------------------------------------
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} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Colleagues,
}
} We have a problem with an older Noran Si(Li) detector running on a
} Voyager
} platform (it is about 19 years old!) that I have not come across
} before.
}
} Suddenly this week instead of getting a single peak, all of the peaks
} in
} the spectrum are split into doublets. For instance for Al K at 1.486keV
} we
} get two peaks separated by ~100eV and centered at 1.486keV.
}
} Has anybody seen this and is there a cure?
}
} Alan
}
}
} --
} Alan W Nicholls, PhD
} Associate Director, RRC
} Director Research Resource Facility - Electron Microscopy
} Research Resources Center, University of Illinois at Chicago
} Rm 110, 845 West Taylor St
} Chicago, IL 60607
} 312 996 1227
} nicholls-at-uic.edu
}
}
} ==============================Original
} Headers==============================
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} 8, 25 -- Date: Thu, 13 Oct 2016 08:28:31 -0500
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==============================Original Headers==============================
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From: steven.spurgeon-at-pnnl.gov
Date: Thu, 13 Oct 2016 12:15:20 -0500
Subject: [Microscopy] LaTeX template for Microscopy and Microanalysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello everyone,

I’m preparing a submission for Microscopy and Microanalysis and I’m looking for a LaTeX template. Does anyone have one they could share or suggestions for another package to use?

Thanks!

______________________________________
Steven R. Spurgeon, Ph.D.
Postdoctoral Research Associate
Physical and Computational Sciences Directorate

Pacific Northwest National Laboratory
902 Battelle Boulevard
P.O. Box 999 MSIN:K8-87
Richland, WA 99352

Tel: +1-509-371-7123
steven.spurgeon-at-pnnl.gov
www.stevenspurgeon.com {http://www.stevenspurgeon.com/}
www.pnnl.gov {http://www.pnnl.gov/}



==============================Original Headers==============================
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From: wesaia-at-iastate.edu
Date: Thu, 13 Oct 2016 17:49:18 -0500
Subject: [Microscopy] Re: XEDS: Problem with peak splitting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Are you talking about overflow in the MCA channels? I know we had an issue with that with a Tracor Northern 2000 system from back around 1980. We had a choice of configuration between 24 bits per channel at 1024 channels or 16 bits per channel at 1536 channels. We opted for the second case and could get rollover in extreme cases. However, I think it looked different that what Nicholl's group has run into.

Warren

________________________________________
X-from: amenex-at-amenex.com {amenex-at-amenex.com}
Sent: Thursday, October 13, 2016 11:53 AM
To: Straszheim, Warren E [BIOTC]

Dear Dr.Nicholls -

Sixteen years out from any contact with Amenex's ETEC Autoprobe, I
recall
seeing line doubling when the detector was getting overloaded,
especially
in my hands. The context is that I was trying to write code in TrueBasic
to automate the process of setting the wavelength-dispersive
spectrometers
at the correct angle to detect the X-rays coming from specific elements,
whose wavelengths are ever so slightly dependent on their chemical
surroundings. The software was to scan across the selected wavelength
and
then select the angle at which the signal was at its maximum. Whenever
the detector gain was excessive, the peak would get folded over onto
itself, producing the false doublet.

Just back off the gain setting.

Hope this helps.

George Langford, Sc.D.
Amenex Associates, Inc.
http://www.amenex.com/
amenex-at-amenex.com
You wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Colleagues,
}
} We have a problem with an older Noran Si(Li) detector running on a
} Voyager
} platform (it is about 19 years old!) that I have not come across
} before.
}
} Suddenly this week instead of getting a single peak, all of the peaks
} in
} the spectrum are split into doublets. For instance for Al K at 1.486keV
} we
} get two peaks separated by ~100eV and centered at 1.486keV.
}
} Has anybody seen this and is there a cure?
}
} Alan
}
}
} --
} Alan W Nicholls, PhD
} Associate Director, RRC
} Director Research Resource Facility - Electron Microscopy
} Research Resources Center, University of Illinois at Chicago
} Rm 110, 845 West Taylor St
} Chicago, IL 60607
} 312 996 1227
} nicholls-at-uic.edu
}
}
} ==============================Original
} Headers==============================
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==============================Original Headers==============================
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From: microscopy.listserver-at-gmail.com
Date: Fri, 14 Oct 2016 06:43:38 -0500
Subject: [Microscopy] viaWWW: CIASEM 2017 Meeting

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Email: ciasem2017-at-imre.uh.cu
Name: Carlos Alberto Lariot

Organization: Cuban Society of Microscopy

Title-Subject: [Filtered] CIASEM 2017

Message: As president of the Cuban Society for Microscopy I have the pleasure to invite you to
participate in the XIV Congress of the Interamerican Committee of Societies for Microscopy CIASEM
2017, to be held in Varadero beach, Cuba, from September 25 to 29, 2017.
Answering to our proposal, Professor Alwyn Eades has wrote the next note:
CIASEM 2017

CIASEM is the Inter-American Committee of Societies for Microscopy. It organizes a Congress every
two years. The next CIASEM Congress will be in Cuba from September 25 to 29 next year. The
organizers are hoping that, now that it is easier for people to travel to Cuba from the USA,
microscopists working in the United States will think to attend.

The Inter-American Congresses on Microscopy bring together scientists from many countries in the
Americas, from Canada to Argentina. They are always good fun. It is my experience that the
discussions at the poster sessions, for example, are far more lively and productive than at other
meetings I have attended. As an added incentive, let me mention that the Congress will be held at
Varadero, a beautiful Caribbean beach resort.


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From: rdpierce-at-pobox.com
Date: Sat, 15 Oct 2016 13:15:52 -0500
Subject: [Microscopy] Info on Leica LEO S430 and Oxford Link ISIS EDX

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

It's been a while since I've posted here. I'd previously been
maintaining a Leica LEO S430 SEM at a makerspace in Chicago. Recently,
we moved the SEM to Analytics Lounge, a new 501(c)(3) non-profit
makerspace in Chicago focused on science education and creating a
laboratory for citizen science. We also have an ICP-MS that we're trying
to bring online, a GFAAS that we're trying to repair, optical
microscopes, and gamma and alpha spectroscopy equipment.

I'd heard that the Oxford Link ISIS was capable of controlling the SEM
and capturing secondary electron video images, and that the quality of
these images was better than what the S430 itself can do, e.g.
1024x768x8 bit.

I don't think we have the necessary hardware for that. Our card cage has
a PULSE PROCESSOR, an X-RAY ADC, and an X-ACQ board. But it seems our
X-ACQ doesn't have a daughterboard that has a "Microscope Digital
Mapping Signal" connector. I'm guessing we'd need the board, and a cable
that would connect to the Video Out, Ext Scan Enable, Ext Line, and Ext
Frame BNC outputs on the S430. And we'd need a serial connection between
the S430 and the Link ISIS. Beyond that, what else would be needed? Is
this something that requires a separate license within the Link ISIS
software? Would there need to be some configuration files specific to
the S430? Assuming we could do this, how much better is the image
resolution and quality?

I've also seen a different configuration of cards when I look for info
on Oxford Link ISIS: a DXP50, XAC-II, and SCANRAM-II. What's the
difference between these and what we have?

Is it possible to obtain Oxford Link ISIS software license files,
considering how old this equipment is? In particular, the installation
we have won't let me run SEMQuant except in demo mode. I don't know if
that was all the original owner purchased, or if we didn't get all the
original licenses installed.

I'm likewise interested in whether it is possible to obtain licenses for
certain features on the LEO S430. E.g. I think beam rotation is an
optional feature that we don't have.

I'm pretty sure we do have the license for RS-232 control of the S430.
But I haven't been able to find any documentation on the API. Is this
available? We've been considering the possibility of interfacing a MIDI
control pad to the SEM via a microcontroller to make it more intuitive
to use than its native Windows 3.1 interface.

I know this is seriously old (ca. 1993) hardware and I'm not sure how
many people are still using it, but any available information would be
much appreciated.

Thanks,

Ryan


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11, 17 -- From: Ryan Pierce {rdpierce-at-pobox.com}
11, 17 -- Subject: Info on Leica LEO S430 and Oxford Link ISIS EDX
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From: drk-at-shcc.org
Date: Sun, 16 Oct 2016 13:32:42 -0500
Subject: [Microscopy] Registration Extended Pacific NW Microscopy Symposium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Fellow Microscopists:

The registration deadline to join the Pacific NW Microscopy Symposium has been extended to 5PM October 27th. This is a two-day symposium to be held November 3rd and 4th in Portland, Oregon. Registration for the Symposium is included with a one-year membership in the Pacific NW Microscopy Society ($25 for professionals; $15 for students) and includes the symposia, workshops, reception and dinner, a farewell wine and cheese social, and all meals during the meeting. The program is absolutely superb. Please go to the PacificNWMicroscopy Website to view the Program and register for the event.

Here is the link: www.pacificnwmicroscopy.org

We will look forward to seeing you in Portland!

Doug


Douglas R. Keene
Assistant Investigator
Micro-Imaging Center
Shriners Hospital for Children
3101 SW Sam Jackson Park Road
Portland, Oregon 97239
Office: 503-221-3434
Mobile: 503-819-3600




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From: dcristofori-at-unive.it
Date: Mon, 17 Oct 2016 04:15:47 -0500
Subject: [Microscopy] Re: Rotary pump on Jeol TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,
Sorry for not replying till now. Quite busy days!
I'd like to thank everybody contributed to the rotary pump substitution
discussion.
We finally decided how to manage the issue, thanks to your comments: as
pointed out by some of you, the control wires doesn't just give
consensus to the alimentation of the pump, but are connected to a sensor
for the rotation of the pulley of the pump. I was able to find the
sensor in the pump. We don't feel comfortable about both modifying the
electronics of the instrument, and introducing a potential danger for
the TEM, so decided to (try to) repair the pump.
Thanks again.

Davide


~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~
Davide Cristofori

direct phone = 041.234.67.26
e-mail = dcristofori[at]unive[dot]it

Centre for Electron Microscopy "Giovanni Stevanato" and
Department of Molecular Sciences and Nanosystems
Ca' Foscari University of Venice

Campus Scientifico, Edificio ETA
Via Torino 155 I-30172 Mestre (VE) Italy
~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~

Il 03/10/2016 19:51, dcristofori-at-unive.it ha scritto:
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} Dear Listers,
} we have a small problem with the rotary pump serving our Jeol JEM-3010.
} Given the age of the pump, we are evaluating whether try to fix the
} problem or buy a new pump. In the latter case, we would prefer to buy a
} different brand than the original one provided with the TEM. But we're
} not sure about the compatibility of the pumps when dealing with the
} control signal used by the TEM electronics to operate the rotary pump.
} Does anyone have experience with such an issue?
} Any contribution will be greatly appreciated.
}
} Davide
}
}
} ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~
} Davide Cristofori
}
} direct phone = 041.234.67.26
} e-mail = dcristofori[at]unive[dot]it
}
} Centro di Microscopia Elettronica "Giovanni Stevanato" e
} Dipartimento di Sc. Molecolari e Nanosistemi
} Universit Ca' Foscari Venezia
}
} Campus Scientifico, Edificio ETA
} Via Torino 155 I-30172 Mestre (VE) Italy
} ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~
}
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} 7, 46 -- From: Davide Cristofori {dcristofori-at-unive.it}
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From: microscopy.listserver-at-gmail.com
Date: Mon, 17 Oct 2016 07:27:22 -0500
Subject: [Microscopy] viaWWW:EDS detector shutter

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Email: massimo.tonelliunimore.it
Name: Massimo Tonelli

Organization: CIGS-University of Modena
Title-Subject: [Filtered] EDS detector shutter

Message: Hello, I need technical help about an old Oxford EDS detector. The detector is installed on
a Jeol 2010 TEM; the microscope has been under maintenance for a long period but now it's fine. The
detector problem is: when I switch ON the PC/interface unit, the software starts and the shutter
seems to be OPEN (?) but when I try to CLOSE it the error message "shutter failure" appears and a
buzzer-alarm sounds. It's necessary to switch off the PC/interface unit to interrupt the alarm.
Anyone ever faced this problem? The shutter system seems a pneumatic system driven through a gray
box mounted on the detector metallic bellow. Anybody knows if is a risky job (for the detector) to
open this box for checking?

Thanks so much in advance,

Massimo


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From: microscopy.listserver-at-gmail.com
Date: Mon, 17 Oct 2016 07:28:12 -0500
Subject: [Microscopy] viaWWW:CIASEM 2017, to be held in Varadero beach, Cuba

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Email: ciasem2017-at-imre.uh.cu Name: Carlos Alberto Lariot

Organization: Organization Committee of the CIASEM 2017

Title-Subject: [Filtered] Announcement
Message: I have the pleasure to invite you to participate in the XIV Congress of the Interamerican
Committee of Societies for Microscopy CIASEM 2017, to be held in Varadero beach, Cuba,
from September 25 to 29, 2017.
Answering to CIASEM 2017 Organization Committee,
Professor Alwyn Eades has wrote the next note:

CIASEM is the Inter-American Committee of Societies for Microscopy. It organizes a Congress every
two years. The next CIASEM Congress will be in Cuba from September 25 to 29 next year. The
organizers are hoping that, now that it is easier for people to travel to Cuba from the USA,
microscopists working in the United States will think to attend.

The Inter-American Congresses on Microscopy bring together scientists from many countries in the
Americas, from Canada to Argentina. They are always good fun. It is my experience that the
discussions at the poster sessions,
for example, are far more lively and productive than at other meetings I have attended. As an
added incentive, let me mention that the Congress will be held at Varadero, a beautiful Caribbean
beach resort.


http://www.ciasem2017.sld.cu/index.php/CIASEM/2017


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From: microscopy.listserver-at-gmail.com
Date: Mon, 17 Oct 2016 07:29:09 -0500
Subject: [Microscopy] viaWWW: Post-doc position available

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Email: andy.stewart-at-ul.ie Name: Andy Stewart

Organization: University of Limerick

Title-Subject: [Filtered] Post-doc position available
Message: Post Doctoral Researcher – Electron Microscopist
LOCATION: University of Limerick
REPORTS TO: Director of Bernal Institute/Nominee of the Director
SALARY: €37,750 - €46,255 p.a. (maximum starting salary €42,394 p.a.)

QUALIFICATIONS: A doctoral degree (level 10 NFQ) in materials science/electron
microscopy/chemistry/physics/ engineering or a related discipline or demonstrably equivalent
experience in a research environment with at least 2 years’ experience in the area of electron
microscopy applied to materials characterisation.

OVERALL PURPOSE OF THE JOB: To manage and develop the electron microscopy suite at the University of
Limerick, to support research activity in the Institute in the area of materials characterisation
and to carry out original and significant research in this area.
Essential Criteria
Applicants must have:
•A doctoral degree (level 10 NFQ) in materials science/electron microscopy/chemistry/physics/
engineering or a related discipline or demonstrably equivalent experience in a research environment
with at least 2 years’ experience in the area of electron microscopy applied to materials
characterisation.
•Hands-on experience in electron microscopy and be able to train new users in this technique.
•Demonstrable knowledge of materials science, particularly related to the areas of materials for
energy and environment, nanomaterials, pharmaceutical materials, biomedical engineering and
biomaterials, and composite materials.
•Evidence of ability to work individually and as part of a team.
•Good oral and written communication skills.
•Potential to write research reports and develop research proposals in the field of materials, in
collaboration with other researchers.
•A strong track record of recent publications in peer reviewed journals, with at least three journal
papers indexed on Web of Science.

Desirable Criteria
•Experience in Raman spectroscopy. •Experience of working with industry partners to solve
industrially relevant materials-science orientated problems.
Instrumentation -- Double aberration corrected, monochromatic FEI Titan Cubed Thermis 30 TEM with
analytical facilities (SuperEDX and QuantumEELS), which will provide a unique capability in Ireland
for ultra-high resolution imaging and spectroscopic analysis of materials.
-- JEOL JEM-2100F with a field emission source, including bright-/dark field imaging, a STEM
attachment, which simultaneously obtains bright field (BF) and high angle annular dark field (HAADF)
images, an SEI/BSE detector, an energy dispersive X-ray analyser and computer controlled 3D
reconstruction high angle tomography. -- JEOL JEM-2011 with a LaB6 source, STEM including
bright-/dark field imaging, an energy dispersive X-ray analyser.
--Hitachi SU-70 scanning electron microscope with a thermal field emission source, back scattered
electron (BSE) detector, energy dispersive X-ray spectroscopy (EDS), wavelength dispersive X-ray
spectroscopy (WDS) and an electron back scattered diffraction detector (EBSD); a coupled SEM-Raman
system for simultaneous chemical characterisation coupled with high resolution imaging
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From: microscopy.listserver-at-gmail.com
Date: Mon, 17 Oct 2016 07:29:52 -0500
Subject: [Microscopy] viaWWW:Site specific protein labeling

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Email: csuparna1-at-gmail.com Name: Suparna

Organization: UTHealth Medical School-Houston

Title-Subject: [Filtered] Site specific protein labeling

Message: Hi All,

Could anyone suggest me what would the best feasible way to label my membrane bound protein of
interest in cell (facing towards the cytosol) to study its orientation and/or interaction with
membrane using single molecule techniques like FRET/ FCCS/FLIM-FRET, etc. I am particularly
interested to study the orientation of a specific domain of the protein with respect to the membrane
in live cell.

} From literatures, I have learnt of applying specific ligand (bound to fluorophore) that would bind to the active site; but that might not work for my case as the protein domain is surface exposed and we don't know of any such ligands binding to that surface. Also, is there any hope of using a small peptide directed to the protein domain (or, the amino acid stretch) bound to fluorophore for this purpose?

Any help will be highly appreciated.

Thanks so much!

Suparna

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From: microscopy.listserver-at-gmail.com
Date: Mon, 17 Oct 2016 07:30:39 -0500
Subject: [Microscopy] viaWWW:EMAS-15/IUMAS-7 2017 meeting

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Email: matthm-at-hotmail.com Name: Mike Mattews

Organization: EMAS/IUMAS

Title-Subject: [Filtered] EMAS-15/IUMAS-7 2017 meeting

Message: The EMAS 2017 15th European Workshop and IUMAS-7 Meeting in Konstanz, Germany. [
07.05.2017 until 11.05.2017]
The primary aim of this combined EMAS Workshop / IUMAS Meeting is to assess the state of the art and
reliability of microbeam analysis techniques. EMAS was founded in 1987 to meet the growing demands
of microanalysis users and scientists across Europe for further education, communication and
professional advice. IUMAS was founded in 1994 to promote world-wide cooperation in all aspects of
microbeam analysis through the organisation of an international meeting on microbeam analysis every
three or four years, and by participating in joint committees with other scientific organisations in
matters relevant to microbeam analysis which are better discussed on a world scale.

The format of the meeting is aimed at maximising transfer of knowledge among the participants and at
providing a comprehensive exhibition of the latest analytical equipment. The programme allows for
adequate time and opportunities for participants to visit the technical exhibitions and hold
discussions with the manufacturers.

The main topics of this, the 15th EMAS European Workshop, are: Pushing the Limits - EPMA; Modelling;
Detector Technologies; Surface Characterisation; Cathodoluminescence; Pushing the Limits - General.
Time will also be devoted to problem orientated applications in material science, geological
science, environmental studies, astrophysics, microelectronics, forensics, cultural heritage and
archaeology, nanomaterials, surfaces and interfaces, catalysts, sensors, ...

For details please go to the WWW site.
https://www.microbeamanalysis.eu/events/event/24-emas-2017-15th-european-workshop-on-modern-developments-and-applications-in-microbeam-analysis-and-iumas-7-meeting


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From: nizets2-at-yahoo.com
Date: Mon, 17 Oct 2016 08:53:43 -0500
Subject: [Microscopy] Re: viaWWW:Site specific protein labeling

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Hi Suparna,

you are putting the bar very high. Considering cell membranes are dynamic and EM techniques require cells to be killed, don't expect it to be an easy task.
I would say the first consideration is the distance between your protein domain of interest and the cell membrane. There is a chance that this distance is too short to be resolvable by classical fluorescence microscopy techniques. Thanks God Stefan Hell came on earth to save fluorescence microscopists and now we have fluorescence microscopes with very high resolution, however there is not always one nearby.
You are talking about FRET, meaning you probably expect distances of several nm. In this case, I don't see which technique would allow you to resolve this distance in live cells. Sure you'll see a fluorescence due to FRET but you won't be able to locate it with respect to the membrane, I am afraid. Please also consider the optical thickness of the plane observed in LM. I am not really up-to-date in confocal microscopy but I think you can at best get optical sections of 100nm, so you are limited in resolution in the Z axis as well.
If distance is not such a big challenge than you can perhaps consider expressing your protein/domain with a fluorescent tag, like GFP or RFP?

Regards,
Stephane


--------------------------------------------
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Subject: [Microscopy] viaWWW:Site specific protein labeling
To: nizets2-at-yahoo.com
Date: Monday, October 17, 2016, 2:37 PM




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Email: csuparna1-at-gmail.com
Name: Suparna

Organization: UTHealth Medical School-Houston

Title-Subject: [Filtered] Site specific protein labeling

Message: Hi All,

Could anyone suggest me what would the best feasible way to
label my membrane bound protein of
interest in cell (facing towards the cytosol) to study its
orientation and/or interaction with
membrane using single molecule techniques like FRET/
FCCS/FLIM-FRET, etc. I am particularly
interested to study the orientation of a specific domain of
the protein with respect to the membrane
in live cell.

} From literatures, I have learnt of applying specific
ligand (bound to fluorophore) that would bind to the active
site; but that might not work for my case as the protein
domain is surface exposed and we don't know of any such
ligands binding to that surface. Also, is there any hope of
using a small peptide directed to the protein domain (or,
the amino acid stretch) bound to fluorophore for this
purpose?

Any help will be highly appreciated.

Thanks so much!

Suparna

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From: microscopy.listserver-at-gmail.com
Date: Wed, 19 Oct 2016 07:33:55 -0500
Subject: [Microscopy] viaWWW:Two faculty positions in Physics/Materials Science available

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Organization: Fayetteville State University

Title-Subject: [Filtered] Two faculty positions in Physics/Materials Science available at
Fayetteville State University

Message: There are two faculty position openings in Physics/Materials Science at Fayetteville State
University:

Assistant/Associate Professor of Physics
https://jobs.uncfsu.edu/postings/14519

Assistant/Associate Professor of Physical Science
https://jobs.uncfsu.edu/postings/14513
FSU houses a field-emission EPMA. Candidates with expertise in EPMA are encouraged to apply.


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From: frank_karl-at-ardl.com
Date: Wed, 19 Oct 2016 09:37:57 -0500
Subject: [Microscopy] change in Link-in Microscopy Group

Contents Retrieved from Microscopy Listserver Archives
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Hello Everyone,
Please forward this note on other listservers that focus on microscopy or aspects of microscopy. I want to reach every member of Linked-In's Microscopy Group (over 5000!) so they have an opportunity to participate in the direction the group is moving. Not everyone checks their Linked-In daily.

The Microscopy Group was formed to connect anyone associated with a microscope with the job market. The primary function of the Microscopy Group is to help find jobs. Transfer of techniques or other information is a desirable, but accidental occurrence. This is your chance to change that.
To accomplish this I allowed headhunters and recruiters to join, as well as anyone with a microscopy connection no matter how minor.

My only requirement is they type a sentence mentioning microscopy, microscope or any of the many techniques that enrich our field. Clicking a box claiming a skill doesn't make the grade.

I started the group, and it's my rules, but I'm retiring, so it could be your rules. Want to be group manager? Contact me and I'll set it up.
What do you think?

Frank Karl
Microscopy Group Manager
Searcher12-at-gmail.com
Or Frank_karl-at-ardl.com


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==============================Original Headers==============================
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From: wtivol-at-sbcglobal.net
Date: Wed, 19 Oct 2016 19:06:42 -0500
Subject: [Microscopy] Re: change in Link-in Microscopy Group

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} On Oct 19, 2016, at 7:51 AM, frank_karl-at-ardl.com wrote:
}
}
} Hello Everyone,
} Please forward this note on other listservers that focus on microscopy or aspects of microscopy. I want to reach every member of Linked-In's Microscopy Group (over 5000!) so they have an opportunity to participate in the direction the group is moving. Not everyone checks their Linked-In daily.
}
} The Microscopy Group was formed to connect anyone associated with a microscope with the job market. The primary function of the Microscopy Group is to help find jobs. Transfer of techniques or other information is a desirable, but accidental occurrence. This is your chance to change that.
} To accomplish this I allowed headhunters and recruiters to join, as well as anyone with a microscopy connection no matter how minor.
}
} My only requirement is they type a sentence mentioning microscopy, microscope or any of the many techniques that enrich our field. Clicking a box claiming a skill doesn't make the grade.
}
} I started the group, and it's my rules, but I'm retiring, so it could be your rules. Want to be group manager? Contact me and I'll set it up.
} What do you think?
}
} Frank Karl
} Microscopy Group Manager

Dear Frank,
I think this makes sense. The listservers for microscopy, 3dem, mod, etc. are good places for technique-related posts, so using LinkedIns group for job-related posts makes a good division of effort.
Yours,
Bill





==============================Original Headers==============================
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7, 34 -- Subject: Re: [Microscopy] change in Link-in Microscopy Group
7, 34 -- From: Bill & Sue Tivol {wtivol-at-sbcglobal.net}
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From: wij.muss-at-aon.at
Date: Thu, 20 Oct 2016 04:46:19 -0500
Subject: [Microscopy] Announcement for June 2017 - SAVE the DATE 44th Ann. Meeting of SCUR - The Skin Imaging Society (Society for Cutaneous Ultrastructure Research) AND AVISO for SSSR-SCUR Joint Meeting Oct. 2018

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Good Morning/Good Day/Good afternoon
Dear 'Listers'
Dear Colleagues,

allow me to send this message for your kind information
(knowing not all MSA-Listserver members might have interest in
dermatological / dermatopathological matters / structural and molecular Skin
research - so -please- be patient]:


SAVE THE DATE
*1st Announcement*
Society for Cutaneous Ultrastructure Research
NEXT MEETING of SCUR - The SKIN IMAGING SOCIETY -

44th ANNUAL MEETING: 8-10th June, 2017 MILANO, ITALY
General Theme:
" INSIDE OUT - OUTSIDE IN
THE SKIN "

You are kindly invited to think about sending your abstract,
or - most welcome - plan to attend the meeting.
[Travel grants - Awards for best Oral - best Poster Presentation]

Further (preliminary) information at: www.scur.org
NB: UPDATE & RELAUNCHING SCUR Website to be expected in November 2016

If you have further request for information - please -
contact the Local Organizer via e-mail: scurmilan2017-at-gmail.com

Best regards and with my compliments


Wolfgang Muss, Ph.D., FRMS
SCUR-BOARD MEMBER
Emeritus member of the MSA
----------------------------------------------------------------------------
---

AVISO for OCTOBER 2018 - SAVE THE DATEs - :
7th Joint Meeting of the Society for Skin Structure Research (SSSR, Japan)
with
SCUR - The Skin Imaging Society

The (Japanese) SOCIETY for SKIN STRUCTURE RESEARCH (SSSR)
AND
The Society for Cutaneous Ultrastructure Research (SCUR) - The SKIN IMAGING
SOCIETY
proudly announce their 7th Joint Meeting (= also 45th Annual Meeting of
SCUR)

to be held October 3rd (half day), 4th (full day), 5th (half day), 2018

VENUE: Asahikawa Grand Hotel, Asahikawa, Japan.

This Joint Meeting takes place prior to
The 82nd Annual Meeting of the Eastern Division of Japanese Dermatology
Association
(October 6th to 7th, 2018, same location).



==============================Original Headers==============================
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18, 20 -- Subject: Announcement for June 2017 - SAVE the DATE 44th Ann. Meeting of SCUR - The Skin Imaging Society (Society for Cutaneous Ultrastructure Research) AND AVISO for SSSR-SCUR Joint Meeting Oct. 2018
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From: oshel1pe-at-cmich.edu
Date: Thu, 20 Oct 2016 07:32:15 -0500
Subject: [Microscopy] Re: change in Link-in Microscopy Group

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

It's a good idea to post jobs on Linked-In, but I strongly urge people to continue posting job openings on the MSA web site - under "Resources" - and here on the list server.

Linked-In is a social media member site that requires registering and providing personal information which is then used for marketing by Linked-In. Basically, another kind of Facebook (if Facebook doesn't already own Linked-In - do they?). With all the privacy caveats one has with Facebook and the like.

Why I don't belong. That, and getting numerous requests from other people I don't know "inviting" me to join Linked-In, and who frequently have nothing to do with microscopy, based on the information provided in the invitation.
I wonder if the people from whom I get these invites even know they're being sent to me?

One should think harder about joining some social site without full knowledge of what that site does with your information and how they make their money. It can only be by selling your information - to corporations and groups you don't know who will use your information in ways you don't know and can't control.

Linked-In may be useful, but let's not require people to go there for their job searches.



Phil

Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576
________________________________________
X-from: wtivol-at-sbcglobal.net {wtivol-at-sbcglobal.net}
Sent: Wednesday, October 19, 2016 20:22
To: Oshel, Philip Eugene


} On Oct 19, 2016, at 7:51 AM, frank_karl-at-ardl.com wrote:
}
}
} Hello Everyone,
} Please forward this note on other listservers that focus on microscopy or aspects of microscopy. I want to reach every member of Linked-In's Microscopy Group (over 5000!) so they have an opportunity to participate in the direction the group is moving. Not everyone checks their Linked-In daily.
}
} The Microscopy Group was formed to connect anyone associated with a microscope with the job market. The primary function of the Microscopy Group is to help find jobs. Transfer of techniques or other information is a desirable, but accidental occurrence. This is your chance to change that.
} To accomplish this I allowed headhunters and recruiters to join, as well as anyone with a microscopy connection no matter how minor.
}
} My only requirement is they type a sentence mentioning microscopy, microscope or any of the many techniques that enrich our field. Clicking a box claiming a skill doesn't make the grade.
}
} I started the group, and it's my rules, but I'm retiring, so it could be your rules. Want to be group manager? Contact me and I'll set it up.
} What do you think?
}
} Frank Karl
} Microscopy Group Manager

Dear Frank,
I think this makes sense. The listservers for microscopy, 3dem, mod, etc. are good places for technique-related posts, so using LinkedIns group for job-related posts makes a good division of effort.
Yours,
Bill


==============================Original Headers==============================
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From: John.Mardinly-at-asu.edu
Date: Thu, 20 Oct 2016 18:13:32 -0500
Subject: [Microscopy] Fwd: change in Link-in Microscopy Group

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


}
} Phil;
} Microsoft bought LinkedIn for $26Bn to make money through the devious methods you describe.
}
} A. John Mardinly, Ph.D., P.E.
} Retired
}
}
}
}
}
}
}


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From: jkrupp-at-deltacollege.edu
Date: Fri, 21 Oct 2016 11:00:41 -0500
Subject: [Microscopy] LR White

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Can someone unconfuse me about how LR White is supplied and used?

I am getting confused by the labels that say ‘catalyzed’ and when to use the separate ‘accelerator’

Is the benzoyl peroxide accelerator only added for doing cold cures?

What is the catalyst that is already mixed in? Does this affect the shelf life or heat cure?

I thought I had this figured out but this morning a student brought me a vial of LR White that had polymerized over night in the refrigerator. No accelerator added, straight from the stock bottle. Can’t figure that one out.

I am now reconfused about this stuff and need some remedial education.

Jon

Jonathan Krupp
ASB&T
San Joaquin Delta College
5151Pacific Ave.
Stockton, CA 95207
(209) 954-5284
jkrupp-at-deltacollege.edu



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From: frank.macaluso-at-einstein.yu.edu
Date: Fri, 21 Oct 2016 14:58:06 -0500
Subject: [Microscopy] EM research technician position available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The Analytical Imaging Facility of Albert Einstein College of Medicine in New York City has an opening for an experienced electron microscopy technician.

The Analytical Imaging Facility (AIF) is a core resource that provides comprehensive, state-of-the-art light and electron microscope imaging technologies and services to scientists at all levels of expertise. The AIF staff supports visual analysis by techniques ranging from fluorescence microscope imaging in 3D to high resolution electron microscopy. A significant effort is devoted to training investigators who require microscopy techniques to advance their research projects.

The successful candidate must be well versed in a variety of standard electron microscopic methods, with two to four years (or equivalent) of experience in electron microscopy. The technician must be proficient in sample preparation techniques for electron microscopy. The ability to operate transmission electron microscopes and scanning electron microscopes is essential. Have working ability in digital imaging and quantitative image analysis. An understanding of scientific literature and moderate understanding of research goals and rationale is required. It is essential that the technician have excellent communication skills. Finally, the technician must be able to work well with many people in a multi-user environment.

For more information and to apply for this position go to:
https://careers-einstein.icims.com/jobs/9808/technician%2c-research-c/job

Visit the lab website:
http://www.einstein.yu.edu/research/shared-facilities/analytical-imaging-facility/


Frank Macaluso
Senior Associate in Anatomy and Structural Biology
Administrative Director and Director of Electron Microscopy
Analytical Imaging Facility
Albert Einstein College of Medicine
1300 Morris Park Avenue
Bronx, NY 10461

(718) 430-3547
frank.macaluso-at-einstein.yu.edu
http://www.einstein.yu.edu/aif

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From: amit.welcomes.u-at-gmail.com
Date: Mon, 24 Oct 2016 00:19:47 -0500
Subject: [Microscopy] [Sum up] Sudden flashes seen on Fluorescent screen of TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Thank you everyone for help. The flashes we observed were indeed due
to fluorescent screen charging, further action awaits administrative
approval!
For completion sake here is the summary:
Problem:
Bright flashes of light were observed with a click sound on
flourescent screen. however on suggestion from listerver and further
analysis:

1. No sound were heard when screen was "up"
2. No change in intensity seen on CCD camera
3. FE electron gun showed no change in intensity (looking through the
window on electron gun)
4. No accelerating voltage fluctuation
5. No vacuum pressure level fluctuation of any kind
6. Flashed were more prominent during alignment (when image intensity
will change a lot and screen will be thoroughly radiated due to
various intensity wobbling etc.)
7. proper corona discharge kind of flash was also seen once on twice
(at the edge of screen)
8. Issue disappeared on own for few weeks, now surfaced again

Current reasoning: In JEOL JEM screen is held by 3 screws (as told by
JEOL engineer) one of which is grounded, looks like ground screw is
bit dodgy as the moment.

Will let everyone know in case any development occurs.

Thank you so much again everyone for help.

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From: PhillipsT-at-missouri.edu
Date: Mon, 24 Oct 2016 08:45:05 -0500
Subject: [Microscopy] today's doodle

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Be sure to check out today's doodle at Google - it features van Leeuwenhoek

Thomas E. Phillips, Ph.D
Curator's Distinguished Teaching Professor of Biological Sciences Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400
573-882-4712 (office)
573-882-0123 (fax)



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4, 36 -- Subject: today's doodle
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From: mdelann1-at-jhmi.edu
Date: Mon, 24 Oct 2016 10:37:49 -0500
Subject: [Microscopy] LR White

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,
LR White, (London resin white) is used mainly for post-embed immuno-
electron microscopy. The cut surface allows for epitopes to be exposed and
labeled with antibodies without the need to etch or antigen retrieval
techniques.
Most times it contains the catalyst so polymerization can be done at 50 C.
Check with the manufacturer but it can also be UV polymerized (you may need
to add a different catalyst). Most samples are lightly fixed ( no high
concentrations of glutaraldehyde)
not osmicated and dehydrated up to 70% ETOH (I usually go to 90% as the 70%
is sometimes difficult to completely mix with the resin). Uranyl acetate
and small amounts of tannic acid may be used in lieu of osmium. Sections
are collected on formvar grids as the resin is beam unstable. Semi thin
sections can also be generated to try immunofluorescence experiments.

Good luck,
Michael


-----Original Message-----
X-from: jkrupp-at-deltacollege.edu [mailto:jkrupp-at-deltacollege.edu]
Sent: Friday, October 21, 2016 12:18 PM
To: mdelann1-at-jhmi.edu

Can someone unconfuse me about how LR White is supplied and used?

I am getting confused by the labels that say b


==============================Original Headers==============================
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From: bfoster-at-the-mip.com
Date: Mon, 24 Oct 2016 13:44:35 -0500
Subject: [Microscopy] Happy Birthday, van Leeuwenhoek!

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Today is Antoni van Leeuwenhoeks 384th Birthday and Google is celebrating it with today's "Google Doodle". VIsit Google and enjoy is view of "the tiny world in a drop of water."

Best regards,
Barbara Foster, President & Chief Consultant

Microscopy/Microscopy Education ... "Education, not Training"
7101 Royal Glen Trail, Suite A - McKinney, TX 75070 - P: 972-924-5310
www.MicroscopyEducation.com

Microscopy/Microscopy Education is a division of The MiP

What are YOUR thoughts on the Lab of the Future?
Give us your input: http://microscopyeducation.com/current-study-lab-of-the-future/


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From: microscopy.listserver-at-gmail.com
Date: Tue, 25 Oct 2016 08:07:06 -0500
Subject: [Microscopy] viaWWW:EM Specialist position at CUNY ASRC

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Email: Tong.Wang-at-asrc.cuny.edu Name: Tong Wang

Organization: Advanced Science Research Center, The City University of New York

Title-Subject: [Filtered] EM Specialist position at CUNY ASRC

Message: Dear Colleagues,

The Imaging Facility of Advanced Science Research Center at The City Univer=
sity of New York is looking for an EM Specialist interested in managing the=
Helios dualbeam FIB-SEM and Titan Themis (S)TEM. The facility houses a Tit=
an Themis 200kV (S)TEM with a Cs image corrector and a superX EDX detector =
and a Helios Nanolab 660 dualbeam FIB-SEM for characterization, sample prep=
aration, and 3D visualization.

More information about this opening can be found here:
https://home.cunyfirst.cuny.edu/psp/cnyepprd/GUEST/HRMS/c/HRS_HRAM.HRS_CE.G=
BL?Page=3DHRS_CE_JOB_DTL&Action=3DA&JobOpeningId=3D15137&SiteId=3D1&Posting=
Seq=3D1#15137
or
http://cuny.jobs/new-york-ny/electron-microscopy-specialist/66DB9772EDAA4A7=
1A188C6167B6DBE20/job/

More information about the CUNY ASRC Imaging Facility can be found here:
http://nanoscience.asrc.cuny.edu/facilities/imaging-suite/

Sincerely,

Tong Wang
Imaging Facility Manager
CUNY Advanced Science Research Center
85 Saint Nicholas Terrace
New York, NY 10031
212-413-3352
Tong.Wang-at-asrc.cuny.edu



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From: les-at-zsgenetics.com
Date: Tue, 25 Oct 2016 16:22:34 -0500
Subject: [Microscopy] STEM Microscopy scientist position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,
ZS Genetics has an opening for a full-time electron microscopy scientist.

This position is in support of technology development for EM-based DNA
sequencing and analysis. The microscopist will be responsible for imaging
activities in aberration corrected Scanning Transmission Electron Microscopy
(AC-STEM) for research and development activities surrounding heavy atom
labeled DNA imaging. The microscopist will participate in design,
implementation, and proving of novel capabilities on the instruments. The
microscopist will participate in automation of imaging and data management
on the instrument. This represents an opportunity to do applications
development in microscopy in a commercial setting.
Please see the full job description at
http://www.zsgenetics.com/open-positions/. Qualified candidates can email
their resume to the address listed on the web page or to myself.

Regards,
Larry Scipioni
ZS Genetics



==============================Original Headers==============================
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From: microscopy.listserver-at-gmail.com
Date: Wed, 26 Oct 2016 17:56:51 -0500
Subject: [Microscopy] viaWWW:Service Engineer for FEI Tecnai

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Email: steve.sem-at-icloud.com Name: Steve Perry

Title-Subject: [Filtered] Service Engineer for FEI Tecnai
Message: I would like to hear from a service engineer who is capable and available to perform
service work on our Tecnai F30.

Located on the West coast USA. Travel and accommodations can be provided if required.

Reply in confidence,



Steve Perry

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From: microscopy.listserver-at-gmail.com
Date: Wed, 26 Oct 2016 17:57:37 -0500
Subject: [Microscopy] viaWWW: updates on "EM Specialist position at CUNY ASRC"

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Email: Tong.Wang-at-asrc.cuny.edu Name: Tong Wang

Organization: Advanced Science Research Center, The City University of New York

Title-Subject: [Filtered] updates on "EM Specialist position at CUNY ASRC"

Message: Dear Colleagues,

The job links in previous message are too long to be displayed as one clickable link. Please use the
following link and search for "Electron Microscopy Specialist":
http://www.asrc.cuny.edu/faculty-opportunities/jobs/

sincerely,

Tong Wang

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From: microscopy.listserver-at-gmail.com
Date: Wed, 26 Oct 2016 17:58:27 -0500
Subject: [Microscopy] viaWWW:Opening for Electron Microscopy staff or postdoc at AIF NCSU

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Email: yliu78-at-ncsu.edu Name: Yang Liu

Organization: Analytical Instrumentation Facility (AIF), North Carolina State Univ

Title-Subject: [Filtered] Opening for Electron Microscopy staff or postdoc at AIF NCSU

Message: The Analytical Instrumentation Facility (AIF) seeks a talented and industrious Electron
Microscopist to join our team as either a postdoc or a full-time staff member (as appropriate for
their experience). Please encourage talented applicants to apply!:
Full-time staff position: https://jobs.ncsu.edu/postings/76529
Postdoc position: https://jobs.ncsu.edu/postings/76522

The AIF is NC State’s primary shared facility for materials characterization with a mission to
enable and lead state-of-the-art research through acquisition, development, maintenance, training,
and access to major analytical and materials characterization instrumentation. Through the support
of engaged faculty and experienced staff, the AIF supports state-of-the-art scanning and
transmission electron microscopes, X-ray scattering and spectroscopy instruments, mass spectrometry,
scanning probe microscopy, nanoindentation, and extensive sample preparation facilities. The AIF is
a core nanotechnology user facility in the new Research Triangle Nanotechnology Network (RTNN), a
site in the National Nanotechnology Coordinated Infrastructure (NNCI).

Primary responsibilities of the new position include training new users (both internal users from NC
State and those external to NC State) as well as performing service for external clients. The ideal
candidate will be customer-focused and exhibit a commitment to excellence in all technical and
organizational aspects of their role. The new position will work closely with the AIF and RTNN teams
in serving the needs of university, industrial, and government researchers from across NC State, the
North Carolina Research Triangle, and the nation.

Questions about the position can be directed to aif-contact-at-ncsu.edu.

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From: microscopy.listserver-at-gmail.com
Date: Wed, 26 Oct 2016 17:59:43 -0500
Subject: [Microscopy] viaWWW:Staff Scientist Position University of Arizona

Contents Retrieved from Microscopy Listserver Archives
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Email: tzega-at-lpl.arizona.edu Name: Tom Zega

Organization: University of Arizona, Kuiper Electron Microscopy Facility

Title-Subject: [Filtered] Staff Scientist Position

Message: Dear Colleagues,
The office of Research, Discovery, and Innovation (RDI) at the University of Arizona invites
applications for an appointed staff scientist position at the Assistant, Associate or (full) Staff
level, dependent on experience, for a soon-to-be installed state-of-the-art transmission electron
microscope (TEM) laboratory. The TEM laboratory is part of RDI Imaging Cores at the University of
Arizona that are dedicated to research, instructional, industrial, and clinical applications.
Services in the electron microscopy facility include Scanning Electron Microscopy (SEM),
Focused-Ion-Beam (FIB) microscopy, and TEM. The scientist will be responsible for user training,
sample preparation, imaging, spectroscopic characterization, instrument maintenance, calibration,
troubleshooting and repair, experimental design, and advanced image/data analysis and archiving for
the TEM laboratory.

Information about this position and how to apply can be found here: https://uacareers.com/postings/14485

Sincerely,

Tom

------------------------------------------------------------
Thomas J. Zega, Ph.D.
Associate Professor
Lunar and Planetary Laboratory
Dept. of Materials Science and Engineering
University of Arizona
1629 E. University Blvd.
Tucson AZ 85721-0092
(v) 520-626-1356
(f) 520-621-4933
https://www.lpl.arizona.edu/PMRG/
------------------------------------------------------------






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From: microscopy.listserver-at-gmail.com
Date: Wed, 26 Oct 2016 18:00:56 -0500
Subject: [Microscopy] viaWWW:POSTDOCTORAL OPENING: SEM in liquids and gases

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Email: andrei.kolmakov-at-nist.gov Name: Andrei Kolmakov

Organization: CNST NIST

Title-Subject: [Filtered] postdoctoral position in EM
Message: Dear Colleagues, please expose the potential candidates to the announcement below. Thank
you in advance.
Andrei Kolmakov
Project Leader CNST NIST Energy Group
NIST 100 Bureau Drive
Gaithersburg, MD 20899-6204
Phone: (301) 975-4724
Fax: (301) 975-2303
E-mail: andrei.kolmakov-at-nist.gov

POSTDOCTORAL OPENING: SEM in liquids and gases

NIST CNST (Center for Nanoscale Science and Technology) has an active research program on
development of in situ scanning electron microscopy and spectroscopy of objects and devices in
liquids and dense gaseous environments using environmental cells equipped with electron transparent
windows (including graphene). We have excellent experimental opportunities for this research line
including clean room micro-fabrication facilities, array of dedicated SEM microscopes (VP JEOL 7800,
UHV Zeiss column coupled with AES capabilities, Zeiss EVO coupled with micro-Raman spectrometer) and
design/engineering support. Currently, a postdoctoral position for highly motivated and
experienced experimentalist is available. The preferable set of skills includes but not limited to:
liquid /atmospheric pressure SEM, electrochemistry, EBIC, XPS, AES, AFM, UHV surface science
protocols, clean room experience,excellent writing and teamwork skills. The letter of interest, CV
with a publication record and contact names of 3-4 references should be sent to Dr. Andrei Kolmakov,
andrei.kolmakov-at-nist.gov Thank you for your interest

Andrei Kolmakov
Project Leader CNST NIST Energy Group
NIST 100 Bureau Drive
Gaithersburg, MD 20899-6204
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From: microwink-at-gmail.com
Date: Wed, 26 Oct 2016 18:43:40 -0500
Subject: [Microscopy] Seeking single tilt holder for EM400 series/CM10/CM12/CM20/CM30 TEM

Contents Retrieved from Microscopy Listserver Archives
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Hello microscopists,

Our 30+ year old single tilt holder, which is the spring-loaded
"donut" clamp, failed after many decades of duty. One of the wires
holder the donut clamp fractured on its own, and thus no longer clamps
samples into the holder. We've had multiple issues across multiple
different microscopes this month; Halloween is indeed a spooky time in
a microscopy lab.

Anyway, does anyone have a donut type clamping single tilt holder
compatible with the EM400, CM10, CM12, CM20, and/or CM30 goniometers
for sale or for donation? We prefer the donut clamp over the circlip
style holder, though we will consider anything on offer/for sale.

Thank you,
Chris

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From: microscopy.listserver-at-gmail.com
Date: Sat, 29 Oct 2016 09:28:46 -0500
Subject: [Microscopy] viaWWW:Post Doctoral Researcher – Electron Microscopist

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-------- Forwarded Message --------

Dear Susan,

not knowing the size of your vein or artery you previously processed (and
how):
Generally, I guess the treatment may have been to harsh with regard to
perhaps the dissection procedure (how was the vessel razor-blade trimmed
prior to fixation? , "full" fixation and perhaps conservative / mild
dehydration etc....
I would not believe 'your' blood clot to disappear simply....Long ago I have
done TEM and SEM studies / specimen preparations on (small and big) vessels
(Arteria thoracica interna) which had to be evaluated for blood clot /
thrombus formation after use of an ultrasonic aspirator/dissector device
(cf:
https://www.researchgate.net/publication/200468940_Effects_of_ultrasound_tre
atment_of_the_arteria_thoracica_Amammaria_interna_ATI_during_preparation_for
_coronary_artery_bypass_surgery_CABG_a_correlative_light_microscopic_scannin
g_and_transmission_elec)
Don't know if you are processing in a tissue processor... where you will
have problems to find smallest pieces of tissue or substrate which are ....
if you process manually in small snap-cap vials made from glass you perhaps
will see such disrupted tissue material more easily.

Blood clots aren't easily/rapidly fixed by the usual procedere, so you might
prolong your primary fixation (use buffered FA-GA fixative) at least 2x as
usual or longer (use of a specimen rotator recommended!) also can use (low
molecular weight) [e.g. 4%, 8%] tannic acid (or sometimes reported: gallic
acid] in combination either with the primary fixative or also use Acrolein
(if that is available for you [CAVE: Caution: Hazard] in combination with
the primary fixative.
Cf. also perhaps to https://www.ncbi.nlm.nih.gov/pubmed/7013112 (just as
only ONE special literature reference)

Best regards and wishes,
Happy Halloween....
Wolfgang

Wolfgang MUSS PhD
Retired from EM-LAb
Emeritus member of MSA
SALZBURG, AUSTRIA




-----Ursprngliche Nachricht-----
Von: microscopy.listserver-at-gmail.com
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Gesendet: Freitag, 28. Oktober 2016 04:45
An: wij.muss-at-aon.at
Betreff: [Microscopy] Embedding blood clots

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Email: andy.stewart-at-ul.ie Name: Andy Stewart

Organization: University of Limerick

Title-Subject: [Filtered] Post Doctoral Researcher – Electron Microscopist (Deadline 2nd November)

Message: TITLE OF POST: Post Doctoral Researcher – Electron Microscopist
LOCATION: University of Limerick
REPORTS TO: Director of Bernal Institute/Nominee of the Director
SALARY: €37,750 - €46,255 p.a. (maximum starting salary €42,394 p.a.)

QUALIFICATIONS: A doctoral degree (level 10 NFQ) in materials science/electron
microscopy/chemistry/physics/ engineering or a related discipline or demonstrably equivalent
experience in a research environment with at least 2 years’ experience in the area of electron
microscopy applied to materials characterisation.

OVERALL PURPOSE OF THE JOB: To manage and develop the electron microscopy suite at the University of
Limerick, to support research activity in the Institute in the area of materials characterisation
and to carry out original and significant research in this area.

Essential Criteria
Applicants must have: •A doctoral degree (level 10 NFQ) in materials science/electron
microscopy/chemistry/physics/ engineering or a related discipline or demonstrably equivalent
experience in a research environment with at least 2 years’ experience in the area of electron
microscopy applied to materials characterisation.•Hands-on experience in electron microscopy
and be able to train new users in this technique.•Demonstrable knowledge of materials science,
particularly related to the areas of materials for energy and environment, nanomaterials,
pharmaceutical materials, biomedical engineering and biomaterials, and composite materials.
•Evidence of ability to work individually and as part of a team.
•Good oral and written communication skills.
•Potential to write research reports and develop research proposals in the field of materials,
in collaboration with other researchers.
•A strong track record of recent publications in peer reviewed journals, with at least three
journal papers indexed on Web of Science.

Desirable Criteria
•Experience in Raman spectroscopy. •Experience of working with industry partners to solve
industrially relevant materials-science orientated problems.

Instrumentation available -- Double aberration corrected, monochromatic FEI Titan Cubed Thermis 30
TEM with analytical facilities (SuperEDX and QuantumEELS), which will provide a unique capability in
Ireland for ultra-high resolution imaging and spectroscopic analysis of materials.

-- JEOL JEM-2100F with a field emission source, including bright-/dark field imaging, a STEM
attachment, which simultaneously obtains bright field (BF) and high angle annular dark field (HAADF)
images, a SEI/BSE detector, an energy dispersive X-ray analyser and computer controlled 3D
reconstruction high angle tomography.
-- JEOL JEM-2011 with a LaB6 source, STEM including bright-/dark field imaging, an energy dispersive
X-ray analyser.

--Hitachi SU-70 scanning electron microscope with a thermal field emission source, back scattered
electron (BSE) detector, energy dispersive X-ray spectroscopy (EDS), wavelength dispersive X-ray
spectroscopy (WDS) and an electron back scattered diffraction detector (EBSD); a coupled SEM-Raman
system for simultaneous chemical characterisation coupled with high resolution imaging

Further information for applicants and application material is available online from:
http://www.ul.ie/hrvacancies/
The closing date for receipt of applications via online completion is, 2nd of November 2016, by 12
noon, Irish Standard Time.

Please email erecruitment-at-ul.ie if you experience any difficulties; queries can also be directed to
Noel.ODowd-at-ul.ie or Ursel.Bangert-at-ul.ie

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From: bicarbaj-at-mtholyoke.edu
Date: Mon, 31 Oct 2016 11:39:53 -0500
Subject: [Microscopy] SEM: career info for undergrads

Contents Retrieved from Microscopy Listserver Archives
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Hello everyone,

This semester I have the pleasure of teaching a hands-on SEM course
for undergraduates. In my last lecture in a couple of weeks, I'd like
to talk a bit about the types of careers in the SEM field. I have a
good idea of the types of jobs in academia and some idea of the more
industry-type jobs, but I think it would be really great if people
could chime in with their specific jobs and what types of samples you
look at. Real world examples are always easier to grasp, I think.

Also, if you know of any certified training courses (besides Lehigh),
please share that information.

Any information is greatly appreciated!

Thank you,
-Blanca

-----------------------------------------
Blanca Carbajal Gonzalez, M.S.
Director of Microscopy
Science Center
50 College St
Mount Holyoke College
Clapp Laboratory 123
Office: 413-538-3118
Cell: 559-905-7138
bicarbaj-at-mtholyoke.edu
MHC Microscopy

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From: microscopy.listserver-at-gmail.com
Date: Tue, 1 Nov 2016 08:44:50 -0500
Subject: [Microscopy] viaWWW: Stanford Imaging Workshop: Advanced Transmission Electron

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Email: alkoh-at-stanford.edu Name: Ai Leen Koh

Organization: Stanford University

Title-Subject: [Filtered] Stanford Imaging Workshop: Advanced Transmission Electron Microscopy for
In-situ and Environmental Studies

Message: Thursday, Dec 8, 2016
8:30 am - 5:15 pm
Stanford University
Stanford Nano Shared Facilities
Stanford, CA 94305-4088
Stanford Nano Shared Facilities (SNSF) invites you to attend this unique workshop demonstrating the
applications of high-speed imaging, electron energy loss spectroscopy, in-situ, and environmental
(scanning) transmission electron microscopy ((S)TEM).
This comprehensive, full-day workshop will feature lectures by leading experts, open Q&A sessions,
and live microscope demonstrations on gas-solid reactions at the state-of-the-art SNSF research
complex. The microscope sessions will be performed on an aberration (image) corrected, monochromated
FEI Titan environmental (S)TEM equipped with a Gatan OneView in-situ camera, Gatan GIF Quantum ERS
imaging filter, plus a Wildfire heating holder and Climate 3+ flow gas system by DENSsolutions.
Registration is free. All confirmed participants will receive a complimentary breakfast, lunch, and
refreshments.
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From: mdelann1-at-jhmi.edu
Date: Tue, 1 Nov 2016 16:37:05 -0500
Subject: [Microscopy] microscopy position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,
We have an opening for a Microsopy Assistant to work at the Johns
Hopkins SOM Microsocpe Facility (est.1989). We are seeking a talented
individual to preform both electron microscopy and some
confocal/fluorescence procedures. We have several electron and confocal
microscopes. This is an excellent opportunity for new graduates to learn
new skills and fine tune your techniques in microscopy.
Please apply through the Johns Hopkins SOM HR dept:

https://jobs.jhu.edu/jhujobs/jobview.cfm?reqId=309189&postId=9811

We look forward to speaking with qualified candidates.

Sincerely,
Michael Delannoy, Assoc. Director
Johns Hopkins SOM Microscope Facility
Baltimore, MD 21205
(410) 955-1365


==============================Original Headers==============================
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5, 20 -- To: {Microscopy-at-microscopy.com} , {microscopy-at-msa.microscopy.com}
5, 20 -- Subject: microscopy position
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From: microscopy.listserver-at-gmail.com
Date: Fri, 4 Nov 2016 05:50:24 -0500
Subject: [Microscopy] viaWWW: European EELS & EFTEM School at Graz University

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Email: jhyun-at-gatan.com Name: John Hyun

Organization: Gatan

Title-Subject: [Filtered] European EELS & EFTEM School at Graz University

Message: The joint FELMI/European EELS & EFTEM School is an intense
4-day hands-on laboratory workshop that will take you step-by-step
through the acquisition and analysis of Energy Filtered TEM (EFTEM) and
STEM-EELS data. The workshop will utilize the state of the art
facilities at FELMI-ZFE including a monochromated probe-corrected Titan
(S)TEM with a DualEELS GIF Quantum EELS system.

Our qualified staff will familiarize you with the latest EELS & EFTEM
equipment and will teach you fundamental principles and methods which
are crucial to take top quality EELS spectra, STEM-EELS spectrum images
and energy-filtered images or elemental maps. While not a focus of the
workshop, optimization of the source monochromator for high-resolution
EELS and the Cs probe corrector for STEM-EELS is included in the program.

For complete details and register, please visit the Graz University
courses website:
http://lampy.tugraz.at/~felmi-zfe/portal/page/portal/felmi/Teaching/LLL-Courses1/GIF%20School2016.html

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From: PhillipsT-at-missouri.edu
Date: Fri, 4 Nov 2016 09:16:29 -0500
Subject: [Microscopy] first color images from TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Check out the exciting TEM advance in the current issue of Cell Chemical Biology. "Detailed view of biological structures is created by overlaying conventional electron micrographs with pseudocolor lanthanide elemental maps derived from distinctive electron energy-loss spectra of each lanthanide deposit via energy-filtered transmission electron microscopy." http://www.cell.com/cell-chemical-biology/fulltext/S2451-9456(16)30357-9



Thomas E. Phillips, Ph.D
Curator's Distinguished Teaching Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400
573-882-4712 (office)
573-882-0123 (fax)
phillipst-at-missouri.edu

http://www.biology.missouri.edu/faculty/phillips.html
http://microscopy.missouri.edu




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From: andrei.kolmakov-at-nist.gov
Date: Fri, 4 Nov 2016 16:51:33 -0500
Subject: [Microscopy] postdoctoral position (liquid SEM) at NIST

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues,
Could you please pass the following information to potential candidates in your group and/or beyond?
Thank you in advance
Andrei Kolmakov

NIST Center for Nanoscale Science and Technology is running an exciting program on electron microscopy (SEM) and spectroscopy (AES, XPS) of objects, interfaces, and devices in liquids and dense gaseous environments using graphene-based and traditional SiN membranes coupled with closed or fluidic cells. In addition to excellent microfabrication facilities, we have an array of dedicated new experimental systems which include: variable pressure SEM, UHV Scanning Auger System, XPS system, micro-Raman /optical spectrometer coupled with SEM as well as setups for in situ electrical measurements. Currently, a postdoctoral position for highly motivated and experienced experimentalist is available. The preferable set of skills includes but not limited: hands-on experience with SEM, XPS, AES, UHV surface science protocols, clean room experience, electrochemistry, excellent writing and teamwork skills and etc.
The letter of interest, CV with publication record and contact information of 3-4 references should be e-mailed to

Dr. Andrei Kolmakov

Thank you for your interest!

Andrei Kolmakov, PhD

Project Leader
Center for Nanoscale Science and Technology
National Institute of Standards and Technology

NIST 100 Bureau Drive
Bldg. 216/Rm.B117
Gaithersburg, MD 20899-6204
Phone: (301) 975-4724
Fax: (301) 975-2303


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From: stefan.diller-at-t-online.de
Date: Sat, 5 Nov 2016 06:11:36 -0500
Subject: [Microscopy] Leica UCT manual

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,

can anybody help me out with a user-manual / and / or service manual in PDF of the Leica UCT ultramicrotome?

Thanks,

Stefan


--


-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.nanoflight.info
www.stefan-diller.com
www.electronmicroscopy.info
www.elektronenmikroskopie.info
www.zwillingsprojekt.de
www.assisi.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------


==============================Original Headers==============================
10, 22 -- From stefan.diller-at-t-online.de Sat Nov 5 06:11:35 2016
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From: vitalylazar-at-att.net
Date: Sun, 6 Nov 2016 16:04:33 -0600
Subject: [Microscopy] specimen drift

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,

Please suggest (a) type of specimen grid / support film and (b)
preparation methods for minimizing specimen drift.
Conditions:
- 120 to 200 kV;
- medium spot size;
- 180kx to 1000kx TEM mag.
- non-organic specimens
I am reasonably sure drift comes from specimen support film, not from
TEM column/stage or specimen holder. Drift will slow down to acceptable
level after sample irradiated by concentrated beam for 10+ seconds or by
wide-spread beam for some 10 minutes - not desirable either way.

--
Vitaly Feingold
SIA
2773 Heath Lane
Duluth GA 30096
Ph. 770-232-7785
Fax 770-232-1791
www.sia-cam.com
vitaly-at-sia-cam.com


==============================Original Headers==============================
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From: mdelann1-at-jhmi.edu
Date: Mon, 7 Nov 2016 08:21:51 -0600
Subject: [Microscopy] specimen drift

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Vitaly,
Have you tried carbon coating your support film (carbon, parlodion or
formvar)? If I get drift on the TEM I will adjust the spot size and/or
flament current to minimize at high magnifications.
Good Luck,
Michael Delannoy

-----Original Message-----
X-from: vitalylazar-at-att.net [mailto:vitalylazar-at-att.net]
Sent: Sunday, November 06, 2016 5:14 PM
To: delannoy-at-jhmi.edu

Dear Listers,

Please suggest (a) type of specimen grid / support film and (b) preparation
methods for minimizing specimen drift.
Conditions:
- 120 to 200 kV;
- medium spot size;
- 180kx to 1000kx TEM mag.
- non-organic specimens
I am reasonably sure drift comes from specimen support film, not from TEM
column/stage or specimen holder. Drift will slow down to acceptable level
after sample irradiated by concentrated beam for 10+ seconds or by
wide-spread beam for some 10 minutes - not desirable either way.

--
Vitaly Feingold
SIA
2773 Heath Lane
Duluth GA 30096
Ph. 770-232-7785
Fax 770-232-1791
www.sia-cam.com
vitaly-at-sia-cam.com


==============================Original Headers==============================
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11, 24 -- From prvs=11236521f=mdelann1-at-jhmi.edu Mon Nov 7 08:21:51 2016
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From: xuanhao.sun-at-uconn.edu
Date: Mon, 7 Nov 2016 08:42:16 -0600
Subject: [Microscopy] Leica UCT manual

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

I can have one of my colleagues scan and send you the pdf tomorrow if you havent received that from other source by that time. Let me know and thanks!

Xuanhao Sun, Ph.D.

Director of Operations
Bioscience Electron Microscopy Laboratory
University of Connecticut
BPB G06, Unit 3242
Storrs, CT 06269-3242

Office Tel: 860-486-6368
Lab Tel: 860-486-2914
Fax: 860-486-6369
website: emlab.uconn.edu


________________________________________
X-from: stefan.diller-at-t-online.de {stefan.diller-at-t-online.de}
Sent: Saturday, November 5, 2016 7:30 AM
To: Sun, Xuanhao

Dear All,

can anybody help me out with a user-manual / and / or service manual in PDF of the Leica UCT ultramicrotome?

Thanks,

Stefan


--


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Arndtstrasse 22
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From: colijn.1-at-osu.edu
Date: Mon, 7 Nov 2016 08:45:07 -0600
Subject: [Microscopy] specimen drift

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Vitaly,

Specimen drift in the TEM generally has 2 causes: thermal or charging.

Thermal drift can arise when the room temperature is different from the
internal temperature of the pole-piece area. When you insert the sample
rod, it can take quite a while to thermally equilibrate. The 2nd cause
of thermal drift can be the local warming of the sample by the e-beam.
There are many aspects of the sample that can affect this: thermal
conductivity of the sample (support film, etc), electron flux, mean free
path of the electrons in the sample, etc.

Sample charging can also lead to drift but it may also lead to more of
an instability as the sample charges up and discharges.

X-from your description, it sounds like local sample heating is the most
likely source of your drift especially if you don't notice drift on
other samples. Using a carbon support film rather than a polymer support
film may improve things.

Cheers,
Henk

--------------------

Hendrik O. Colijn
Center for Electron Microscopy and AnalysiS
The Ohio State University
1305 Kinnear Rd, Suite 100, Columbus, OH 43212

colijn.1-at-osu.edu 614/643-3458
cemas.osu.edu {about:cemas.osu.edu}

"Time is that quality of nature which keeps things from happening all at
once." (Ray Cummings - 1922)
Lately it doesn't seem to be working.



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To: colijn.1-at-osu.edu
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From: stefan.diller-at-t-online.de
Date: Mon, 7 Nov 2016 10:07:31 -0600
Subject: [Microscopy] Re: Leica UCT manual

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,

thanks for getting the manual. No more help needed.

This group is one-of-a-kind ;-)


Best,

Stefan

-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.nanoflight.info
www.stefan-diller.com
www.electronmicroscopy.info
www.elektronenmikroskopie.info
www.zwillingsprojekt.de
www.assisi.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------

Am 07.11.16 um 15:41 schrieb Sun, Xuanhao:
} Hi,
}
} I can have one of my colleague scan and send you the pdf tomorrow if you havent received that from other source by that time.
} Let me know and thanks!
}
} Xuanhao Sun, Ph.D.
}
} Director of Operations
} Bioscience Electron Microscopy Laboratory
} University of Connecticut
} BPB G06, Unit 3242
} Storrs, CT 06269-3242
}
} Office Tel: 860-486-6368
} Lab Tel: 860-486-2914
} Fax: 860-486-6369
} website: emlab.uconn.edu {http://emlab.uconn.edu}
}
} } On Nov 5, 2016, at 7:30 AM, stefan.diller-at-t-online.de {mailto:stefan.diller-at-t-online.de} wrote:
} }
} }
} }
} }
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
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} } ----------------------------------------------------------------------------
} }
} } Dear All,
} }
} } can anybody help me out with a user-manual / and / or service manual in PDF of the Leica UCT ultramicrotome?
} }
} } Thanks,
} }
} } Stefan
} }
} }
} } --
} }
} }
} } -----------------------------------------------------
} } Stefan Diller - Scientific Photography
} } Arndtstrasse 22
} } D - 97072 Wuerzburg Germany
} } ++49-931-7848700 Phone
} } ++49-931-7848701 Fax
} } ++49-175-7177051 Mobile
} }
} } Websites:
} } www.nanoflight.info {http://www.nanoflight.info}
} } www.stefan-diller.com
} } www.electronmicroscopy.info
} } www.elektronenmikroskopie.info
} } www.zwillingsprojekt.de
} } www.assisi.de
} } Anfahrt: http://Mail.map24.com/Stefan.Diller
} } -----------------------------------------------------
} }
} }
} } ==============================Original Headers==============================
} } 10, 22 -- From stefan.diller-at-t-online.de Sat Nov 5 06:11:35 2016
} } 10, 22 -- Received: from mailout01.t-online.de (mailout01.t-online.de [194.25.134.80])
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} } 10, 22 -- To: microscopy-at-microscopy.com
} } 10, 22 -- From: Stefan Diller {stefan.diller-at-t-online.de}
} } 10, 22 -- Subject: Leica UCT manual
} } 10, 22 -- Message-ID: {ef5a39af-14d3-1f13-eac4-8657fee7485d-at-t-online.de}
} } 10, 22 -- Date: Sat, 5 Nov 2016 12:12:17 +0100
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} } ==============================End of - Headers==============================
}




==============================Original Headers==============================
12, 27 -- From stefan.diller-at-t-online.de Mon Nov 7 10:07:30 2016
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12, 27 -- esmtp id 1c3mT7-0rqYme0; Mon, 7 Nov 2016 17:08:21 +0100
12, 27 -- Subject: Re: [Microscopy] Leica UCT manual
12, 27 -- To: "Sun, Xuanhao" {xuanhao.sun-at-uconn.edu}
12, 27 -- References: {201611051130.uA5BUPel012822-at-ns.microscopy.com}
12, 27 -- {4A49E31A-D06C-442D-8D8C-5D6BED10DD16-at-uconn.edu}
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12, 27 -- From: Stefan Diller {stefan.diller-at-t-online.de}
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From: FMonson-at-wcupa.edu
Date: Mon, 7 Nov 2016 22:53:45 -0600
Subject: [Microscopy] Leica UCT manual

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I did it today and will send after voting tomorrow.

Fred

Frederick C Monson, PhD
Center for Microanalysis and Imaging, Research and Training (CMIRT)
West Chester University of Pannsylvania
West Chester, PA, 1938
610-738-0437
fmonson-at-wcupa.edu
________________________________________
X-from: xuanhao.sun-at-uconn.edu [xuanhao.sun-at-uconn.edu]
Sent: Monday, November 7, 2016 9:53 AM
To: Monson, Frederick

Hi,

I can have one of my colleagues scan and send you the pdf tomorrow if you havent received that from other source by that time. Let me know and thanks!

Xuanhao Sun, Ph.D.

Director of Operations
Bioscience Electron Microscopy Laboratory
University of Connecticut
BPB G06, Unit 3242
Storrs, CT 06269-3242

Office Tel: 860-486-6368
Lab Tel: 860-486-2914
Fax: 860-486-6369
website: emlab.uconn.edu


________________________________________
X-from: stefan.diller-at-t-online.de {stefan.diller-at-t-online.de}
Sent: Saturday, November 5, 2016 7:30 AM
To: Sun, Xuanhao

Dear All,

can anybody help me out with a user-manual / and / or service manual in PDF of the Leica UCT ultramicrotome?

Thanks,

Stefan


--


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Arndtstrasse 22
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Websites:
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From: lemaster-at-ppg.com
Date: Wed, 9 Nov 2016 11:40:30 -0600
Subject: [Microscopy] SEM - Safety of chromium sputter coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Is it safer to have the sputter coater in a fume hood when sputter coating with chromium?
(As opposed to just sitting on the lab bench)
This comes up because of a request to sputter coat a large number of test strips with chromium.
I couldn't find any safety warnings in this regard, and I don't think there is much risk.
Gloves will be worn when handling the strips.
Any advice would be much appreciated.

J. Scott LeMaster
Senior Research Chemist II
Analytical
PPG


________________________________

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From: microscopy.listserver-at-gmail.com
Date: Thu, 10 Nov 2016 03:22:04 -0600
Subject: [Microscopy] viaWWW:MPFI Neuroimaging Course - EM module

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Email: naomi.kamasawa-at-mpfi.org Name: Naomi Kamasawa

Organization: Max Planck Florida Institute for Neuroscience

Title-Subject: [Filtered] MPFI Neuroimaging Course - EM module

Message: Max Planck Florida Institute for Neuroscience will host an
intensive and comprehensive laboratory-oriented course focusing on
applying imaging techniques to neuroscience research. The objective of
this imaging course is to gain exposure to modern imaging tools from
principle optics to applications in modern neuroscience.

Please see details;
https://www.maxplanckflorida.org/education/courses/LM-course/NIT-2017

Electron Microscopy Facility will host a "correlative LM-EM" module in
the course. EM module attendees have full access to all talks offered in
the course and will focus on CLEM projects. Attendees are encouraged to
bring own their samples upon discussion and approval.
Registration Deadline; November 28, 2016

Contact: Jessica Herbst for general questions (jessica.herbst-at-mpfi.org)
Contact: Naomi Kamasawa (naomi.kamasawa-at-mpfi.org) for EM module questions


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From: microscopy.listserver-at-gmail.com
Date: Thu, 10 Nov 2016 03:25:14 -0600
Subject: [Microscopy] viaWWW:infiltrating large samples

Contents Retrieved from Microscopy Listserver Archives
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Email: buchsmith-at-gmail.com Name: JoAnn Buchanan

Organization: Allen Institute for Brain Science

Title-Subject: [Filtered] infiltrating large samples

Message: Hello listers. We have very large brain samples that are
loaded with heavy metals, 1.5 mm in thickness. Good infiltration is
imperative. I am using Hard Plus resin because of its consistency I
cannot work with Spurr as I am allergic to it. The intermediate fluid is
acetonitrile.

Infiltrating in resin without accelerator seems best, my question is,
which step of the infiltration process should be the most effective -
2:1, 1;1. 1:2, 100%. Right now each step is 24-48 hours except fresh
100% w accelerator is 4-6 hr. Overall protocol is 9 days with 96 hours
of block hardening.

Right now our blocks have small ripped areas in the semi thins. Overall
infiltration is OK, but I need to get rid of these chipped/ripped areas.

I am thinking longer in 100% resin with accelerator before embedding.
Any thoughts, ideas?

thanks in advance. JoAnn

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From: nizets2-at-yahoo.com
Date: Fri, 11 Nov 2016 02:35:20 -0600
Subject: [Microscopy] Re: SEM - Safety of chromium sputter coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi!

As far as I know chromium in its metallic form is not considered as toxic otherwise everyone should handle stainless steel with latex gloves:-).
But then your sputter coater has a vacuum pump right? So you should care about the exhaust in this case.
Just the 2 cents of a cell biologist who was once an electron microscopist.

regards
Stephane


--------------------------------------------
On Wed, 11/9/16, lemaster-at-ppg.com {lemaster-at-ppg.com} wrote:

Subject: [Microscopy] SEM - Safety of chromium sputter coater
To: nizets2-at-yahoo.com
Date: Wednesday, November 9, 2016, 6:48 PM




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Is it safer to have the sputter coater in a fume hood when
sputter coating with chromium?
(As opposed to just sitting on the lab bench)
This comes up because of a request to sputter coat a large
number of test strips with chromium.
I couldn't find any safety warnings in this regard, and I
don't think there is much risk.
Gloves will be worn when handling the strips.
Any advice would be much appreciated.

J. Scott LeMaster
Senior Research Chemist II
Analytical
PPG


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From: WHITTAKS-at-si.edu
Date: Mon, 14 Nov 2016 12:41:46 -0600
Subject: [Microscopy] LM/SEM- Microscopy Educator Position- Wash. DC

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Please see the below posting if you are interested in putting together informal educational programs for youth centered around the use of advanced microscopy. We have some of the newest and most advanced instrumentation available and want to put it in the hands of young learners.

Help Us Create Amazing Microscopy Programs for Museum Audiences!
The Smithsonian National Museum of Natural History is looking for a Microscopy Educator. This is a two year term limited full time staff position at the National Museum of Natural History in Washington DC. We are looking for the right person to produce a set of experiential activities, programs and training workshops about microscope technology and natural history science for a diversity of non-specialist audiences, specifically youth ages 14-18 and their families and teachers. We are looking for a unique individual who has a mix of skills and experiences pertaining to microscopy, informal science learning environments, and teen audiences to be a part of the Education and Outreach team. If you or someone you know has the following skills and experiences, please be in touch. We are looking for individuals with the following:

Microscopy
$B!|(B Extensive knowledge of light, digital and electron microscopy, including troubleshooting problems with microscope hardware and software.
$B!|(B Experience training different audiences to use various types of microscopes.
$B!|(B Experience in the development and implementation of educational programming that communicates microscopy$B!G(Bs history, structure, function and techniques in innovative and engaging ways to highly diverse audiences, including integrating art, design and multiple types of media and educational techniques.
$B!|(B Experience using various imaging editing software programs.

Natural Science
$B!|(B Familiarity with natural history science, including experience working with scientists, research collections, or with in-depth science content.
$B!|(B Interest in the content and collections of the National Museum of Natural History and in connecting microscopy program ideas to exhibits, current science research, specimens and cultural artifacts.

Informal Science Education
$B!|(B Experience in developing activities, programs, and training workshops in an informal learning environment
$B!|(B Experience in communicating science concepts and scientific processes to general audiences and teen audiences effectively.
$B!|(B Experience working with digital media and with teachers and curricula for schools is a plus.

Working with Teens
$B!|(B Experience teaching/facilitating programs for youth audiences (age range for these programs are 13-19).
$B!|(B Excellent at facilitating fun, engaging experiences for teens that invite them to fully and creatively participate
$B!|(B Ability to connect with underserved youth
$B!|(B Experience developing curriculum that encompasses an interdisciplinary and user-led approach.


Interested? Please send a note that outlines your experience in relation to the list above, and CV to Gale Robertson at robertsong-at-si.edu, by Monday November 28, 2016. We may not be able to respond to all inquiries.




Scott Whittaker
Lab Manager
Imaging
P.O. Box 37012 MRC-104 Room W150
Washington, DC 20013-7012
w 202.633.0891 whittaks-at-si.edu

SMITHSONIAN INSTITUTION
NATIONAL MUSEUM OF NATURAL HISTORY
Facebook | Twitter | Instagram




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From: Reinhard.Rachel-at-biologie.uni-regensburg.de
Date: Tue, 15 Nov 2016 04:39:13 -0600
Subject: [Microscopy] Pirani

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues,
on our Edwards Scancoat Six (now about 16 yrs old), the Pirani gauge is faulty and needs to be replaced (or does anybody know how to repair? we would need the W wire - can it be replaced? easy? is it W?) .
It is an Edwards PRE10K (D02428000) - and we asked Edwards and they told us that this Pirani is not available - any more. Hmm.
Does anybody have one for sale which is not used any more?
Does anybody know another type which would fit into the Scancoat Six?
Any help is much appreciated.
kind regards,
Reinhard Rachel

--
Prof. Dr. Reinhard Rachel
University of Regensburg
Centre for EM / Anatomy
Faculty of Biology & Preclin. Med.
Universitaetsstrasse 31
D-93053 Regensburg - Germany
tel +49 941 943 -2837, -1720
mail reinhard.rachel-at-biologie.uni-regensburg.de
office: VKL 3.1.29

Next microscopy conferences:
- Microscopy Conference 2017 (D-A-CH) Lausanne, CH
20-25 August 2017
http://www.mc2017.ch/
- 19th International Microscopy Congress, Sydney; 9-14 Sept 2018
http://imc19.com/
- next Microbiol. conferences:
VAAM/DGHM - Annual Conf
March 5-8, 2017, Wuerzburg





==============================Original Headers==============================
6, 23 -- From Reinhard.Rachel-at-biologie.uni-regensburg.de Tue Nov 15 04:39:13 2016
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6, 23 -- From: "Reinhard Rachel" {Reinhard.Rachel-at-biologie.uni-regensburg.de}
6, 23 -- To: "microscopy server" {Microscopy-at-microscopy.com}
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From: mmoller-at-cicbiomagune.es
Date: Tue, 15 Nov 2016 09:06:26 -0600
Subject: [Microscopy] RE: Pirani

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello!
To my knowledge, after Edwards dropped this kind of equipment from their portfolio, the Indian company HHV continues to market these instruments, and to sell spare parts for it. I once heard rumors that HHV already before would have been the place where this Edwards equipment was built. If this would be true, then contacting HHV would be the way to contact the manufacturer directly ;-) . There webpage states: "We hold an exclusive licence from Edwards to manufacture and supply the well-known ScanCoat Six, Auto306, Auto500 and forensic deposition systems under the HHV brand and have added our own range of systems to our product line-up." (http://www.hhvltd.com/).
Good Luck to find your spare parts there!
Best greetings from the EM-Labs of CIC biomaGUNE,
Marco Mller


----------------------------------------------------------------
Marco Mller
Platform Manager - Electron Microscopy

CIC biomaGUNE
Parque Tecnolgico de San Sebastin
Paseo Miramn, 182. Ed. Empresarial C
20009 San Sebastin (Guipzcoa)
SPAIN

Tel: +34 943 00 53 25
mmoller-at-cicbiomagune.es


} -----Original Message-----
} From: Reinhard.Rachel-at-biologie.uni-regensburg.de
} [mailto:Reinhard.Rachel-at-biologie.uni-regensburg.de]
} Sent: Tuesday, November 15, 2016 11:54 AM
} To: Marco Moller
} Subject: [Microscopy] Pirani
}
}
}
}
} -----------------------------------------------------------------------
} -----
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
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} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
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} -----
}
} Dear Colleagues,
} on our Edwards Scancoat Six (now about 16 yrs old), the Pirani gauge is
} faulty and needs to be replaced (or does anybody know how to repair? we
} would need the W wire - can it be replaced? easy? is it W?) .
} It is an Edwards PRE10K (D02428000) - and we asked Edwards and they
} told us that this Pirani is not available - any more. Hmm.
} Does anybody have one for sale which is not used any more?
} Does anybody know another type which would fit into the Scancoat Six?
} Any help is much appreciated.
} kind regards,
} Reinhard Rachel
}
} --
} Prof. Dr. Reinhard Rachel
} University of Regensburg
} Centre for EM / Anatomy
} Faculty of Biology & Preclin. Med.
} Universitaetsstrasse 31
} D-93053 Regensburg - Germany
} tel +49 941 943 -2837, -1720
} mail reinhard.rachel-at-biologie.uni-regensburg.de
} office: VKL 3.1.29
}
} Next microscopy conferences:
} - Microscopy Conference 2017 (D-A-CH) Lausanne, CH
} 20-25 August 2017
} http://www.mc2017.ch/
} - 19th International Microscopy Congress, Sydney; 9-14 Sept 2018
} http://imc19.com/
} - next Microbiol. conferences:
} VAAM/DGHM - Annual Conf
} March 5-8, 2017, Wuerzburg
}
}
}
}
}
} ==============================Original
} Headers==============================
} 6, 23 -- From Reinhard.Rachel-at-biologie.uni-regensburg.de Tue Nov 15
} 04:39:13 2016
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} 6, 23 -- From: "Reinhard Rachel" {Reinhard.Rachel-at-biologie.uni-
} regensburg.de}
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==============================Original Headers==============================
8, 41 -- From mmoller-at-cicbiomagune.es Tue Nov 15 09:06:21 2016
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8, 41 -- From: Marco Moller {mmoller-at-cicbiomagune.es}
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8, 41 -- CC: "Reinhard.Rachel-at-biologie.uni-regensburg.de"
8, 41 -- {Reinhard.Rachel-at-biologie.uni-regensburg.de}
8, 41 -- Subject: RE: Pirani
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From: mmoller-at-cicbiomagune.es
Date: Tue, 15 Nov 2016 09:11:58 -0600
Subject: [Microscopy] RE: Pirani

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello!
To my knowledge, after Edwards dropped this kind of equipment from their portfolio, the Indian company HHV continues to market these instruments, and to sell spare parts for it. I once heard rumors that HHV already before would have been the place where this Edwards equipment was built. If this would be true, then contacting HHV would be the way to contact the manufacturer directly ;-) . There webpage states: "We hold an exclusive licence from Edwards to manufacture and supply the well-known ScanCoat Six, Auto306, Auto500 and forensic deposition systems under the HHV brand and have added our own range of systems to our product line-up." (http://www.hhvltd.com/).
Good Luck to find your spare parts there!
Best greetings from the EM-Labs of CIC biomaGUNE, Marco Mller.

----------------------------------------------------------------
Marco Mller
Platform Manager - Electron Microscopy
CIC biomaGUNE
Parque Tecnolgico de San Sebastin
Paseo Miramn, 182. Ed. Empresarial C
20009 San Sebastin (Guipzcoa)
SPAIN
Tel: +34 943 00 53 25
mmoller-at-cicbiomagune.es



}
} Dear Colleagues,
} on our Edwards Scancoat Six (now about 16 yrs old), the Pirani gauge
} is faulty and needs to be replaced (or does anybody know how to
} repair? we would need the W wire - can it be replaced? easy? is it W?) .
} It is an Edwards PRE10K (D02428000) - and we asked Edwards and they
} told us that this Pirani is not available - any more. Hmm.
} Does anybody have one for sale which is not used any more?
} Does anybody know another type which would fit into the Scancoat Six?
} Any help is much appreciated.
} kind regards,
} Reinhard Rachel
}


==============================Original Headers==============================
6, 37 -- From mmoller-at-cicbiomagune.es Tue Nov 15 09:11:57 2016
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6, 37 -- Subject: RE: Pirani
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From: mdelann1-at-jhmi.edu
Date: Tue, 15 Nov 2016 09:42:20 -0600
Subject: [Microscopy] RE: Pirani

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Reinhard,
If you can dismantle the gauge and get to the filament you can try
sonicating in mild HCL solution. I do this routinely with my penning gauge
on my FESEM when it gets coated with carbon.
Sincerely,

Michael Delannoy

-----Original Message-----
X-from: mmoller-at-cicbiomagune.es [mailto:mmoller-at-cicbiomagune.es]
Sent: Tuesday, November 15, 2016 10:16 AM
To: delannoy-at-jhmi.edu

Hello!
To my knowledge, after Edwards dropped this kind of equipment from their
portfolio, the Indian company HHV continues to market these instruments, and
to sell spare parts for it. I once heard rumors that HHV already before
would have been the place where this Edwards equipment was built. If this
would be true, then contacting HHV would be the way to contact the
manufacturer directly ;-) . There webpage states: "We hold an exclusive
licence from Edwards to manufacture and supply the well-known ScanCoat Six,
Auto306, Auto500 and forensic deposition systems under the HHV brand and
have added our own range of systems to our product line-up."
(http://www.hhvltd.com/).
Good Luck to find your spare parts there!
Best greetings from the EM-Labs of CIC biomaGUNE, Marco Mvller


----------------------------------------------------------------
Marco Mvller
Platform Manager - Electron Microscopy

CIC biomaGUNE
Parque Tecnolsgico de San Sebastian
Paseo Miramsn, 182. Ed. Empresarial C
20009 San Sebastian (Guipzzcoa)
SPAIN

Tel: +34 943 00 53 25
mmoller-at-cicbiomagune.es


} -----Original Message-----
} From: Reinhard.Rachel-at-biologie.uni-regensburg.de
} [mailto:Reinhard.Rachel-at-biologie.uni-regensburg.de]
} Sent: Tuesday, November 15, 2016 11:54 AM
} To: Marco Moller
} Subject: [Microscopy] Pirani
}
}
}
}
} ----------------------------------------------------------------------
} -
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}
} Dear Colleagues,
} on our Edwards Scancoat Six (now about 16 yrs old), the Pirani gauge
} is faulty and needs to be replaced (or does anybody know how to
} repair? we would need the W wire - can it be replaced? easy? is it W?) .
} It is an Edwards PRE10K (D02428000) - and we asked Edwards and they
} told us that this Pirani is not available - any more. Hmm.
} Does anybody have one for sale which is not used any more?
} Does anybody know another type which would fit into the Scancoat Six?
} Any help is much appreciated.
} kind regards,
} Reinhard Rachel
}
} --
} Prof. Dr. Reinhard Rachel
} University of Regensburg
} Centre for EM / Anatomy
} Faculty of Biology & Preclin. Med.
} Universitaetsstrasse 31
} D-93053 Regensburg - Germany
} tel +49 941 943 -2837, -1720
} mail reinhard.rachel-at-biologie.uni-regensburg.de
} office: VKL 3.1.29
}
} Next microscopy conferences:
} - Microscopy Conference 2017 (D-A-CH) Lausanne, CH
} 20-25 August 2017
} http://www.mc2017.ch/
} - 19th International Microscopy Congress, Sydney; 9-14 Sept 2018
} http://imc19.com/
} - next Microbiol. conferences:
} VAAM/DGHM - Annual Conf
} March 5-8, 2017, Wuerzburg
}
}
}
}
}
} ==============================Original
} Headers==============================
} 6, 23 -- From Reinhard.Rachel-at-biologie.uni-regensburg.de Tue Nov 15
} 04:39:13 2016
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} Date: Tue, 15 Nov 2016 11:40:30 +0100 6, 23 -- From: "Reinhard Rachel"
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} server" {Microscopy-at-microscopy.com} 6, 23 -- Subject: Pirani 6, 23 --
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From: microscopy.listserver-at-gmail.com
Date: Tue, 15 Nov 2016 11:09:03 -0600
Subject: [Microscopy] viaWWW:How to do SEM EDS on embedded teeth

Contents Retrieved from Microscopy Listserver Archives
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X-from: chrisbrantner-at-gwu.edu

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Email: chrisbrantner-at-gwu.edu Name: Chris Brantner

Organization: George Washington University

Title-Subject: [Filtered] How to do SEM EDS on embedded teeth

Message: Hi all,

We would like to look at some special teeth samples and to determine the
elements in a cross-section sawed out of these samples. The teeth have
been embedded in methyl salicylate. The cross sections will be 100
microns thick. Some historical samples have been placed on glass slides
and cover slipped or plastic slides and overslipped. I could use some
hints about how to obtain an elemental map from this material.

Thanks
Chris Brantner George Washington University
Nanofabrication and Imaging Center

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From: vray-at-partbeamsystech.com
Date: Tue, 15 Nov 2016 12:05:56 -0600
Subject: [Microscopy] Re: Pirani

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Reinhard,

Pirani can be repaired if you really wanted to do it, you can DIY after
reading some of books and papers on vacuum instrumentation and
techniques, or contact some of the places that routinely repair vacuum
instrumentation. Examples would be Duniway Stockrom (duniway.com), or
VGM Inc. (vgminc.net), or Scientific Instrument Services (sisweb.com) -
I am sure there are also plenty of places capable of repairing Pirani in
Europe as well, Google is your friend.

Replacement PRE10K also available elsewhere: there are four of these
gauges listed on E*Bay, also check Labsource (labsoruce.come) and PLC
Center (plccenter.com).

Good luck!
Valery

Valery Ray
www.linkedin.com/in/valeryray
==============================
PBS&T, MEO Engineering Company
290 Broadway, Suite 298
Methuen, MA 01844, USA
Phone: +1-978-305-0479 - leave a message
Mobie: +1-978-305-0479 - leave a message
E-mail: vray-at-partbeamsystech.com
Web: www.partbeamsystech.com
Web: www.freudlabs.com

On 11/15/2016 5:40 AM, Reinhard.Rachel-at-biologie.uni-regensburg.de wrote:
} ----------------------------------------------------------------------------
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} Dear Colleagues,
} on our Edwards Scancoat Six (now about 16 yrs old), the Pirani gauge is faulty and needs to be replaced (or does anybody know how to repair? we would need the W wire - can it be replaced? easy? is it W?) .
} It is an Edwards PRE10K (D02428000) - and we asked Edwards and they told us that this Pirani is not available - any more. Hmm.
} Does anybody have one for sale which is not used any more?
} Does anybody know another type which would fit into the Scancoat Six?
} Any help is much appreciated.
} kind regards,
} Reinhard Rachel
}

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From: bigelow-at-umich.edu
Date: Tue, 15 Nov 2016 14:03:15 -0600
Subject: [Microscopy] [Mocrpscp[u]RE: Pirani Gauge

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I might suggest checking with Duniway Stockroom Co. They sell and
service all kinds of vacuum gauges, and may be able to help you.
(www.duniway.com)

--
Wilbur C. Bigelow, PhD, LCDR USNR (Ret)
Professor Emeritus of Materials Engineering
The University of Michigan
2911 Whittier Court, Ann Arbor MI 48104


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From: John.Mardinly-at-asu.edu
Date: Tue, 15 Nov 2016 15:01:08 -0600
Subject: [Microscopy] Re: Pirani

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Maybe trump can make it great again. Mail to:

1600 Pennsylvania Ave NW, Washington, DC 20500



} On Nov 15, 2016, at 8:55 AM, mdelann1-at-jhmi.edu wrote:
}
}
}
}
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} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
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} ----------------------------------------------------------------------------
}
} Reinhard,
} If you can dismantle the gauge and get to the filament you can try
} sonicating in mild HCL solution. I do this routinely with my penning gauge
} on my FESEM when it gets coated with carbon.
} Sincerely,
}
} Michael Delannoy
}
} -----Original Message-----
} X-from: mmoller-at-cicbiomagune.es [mailto:mmoller-at-cicbiomagune.es]
} Sent: Tuesday, November 15, 2016 10:16 AM
} To: delannoy-at-jhmi.edu
} Subject: [Microscopy] RE: Pirani
}
}
}
}
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} ----------------------------------------------------------------------------
}
} Hello!
} To my knowledge, after Edwards dropped this kind of equipment from their
} portfolio, the Indian company HHV continues to market these instruments, and
} to sell spare parts for it. I once heard rumors that HHV already before
} would have been the place where this Edwards equipment was built. If this
} would be true, then contacting HHV would be the way to contact the
} manufacturer directly ;-) . There webpage states: "We hold an exclusive
} licence from Edwards to manufacture and supply the well-known ScanCoat Six,
} Auto306, Auto500 and forensic deposition systems under the HHV brand and
} have added our own range of systems to our product line-up."
} (https://urldefense.proofpoint.com/v2/url?u=http-3A__www.hhvltd.com_&d=CwIBAg&c=AGbYxfJbXK67KfXyGqyv2Ejiz41FqQuZFk4A-1IxfAU&r=MAuGvnWTcVQkxORgQD0QS50ZicPM3Nw-61ygSK-LNEQ&m=FA-wvmbqt9CTVfjWXjsi2QEA_w65rF7UvcWrmrhTFks&s=DIWX4B-uub8VIb6xs6hwYO60NH82ro2kq554fy3aXlw&e= ).
} Good Luck to find your spare parts there!
} Best greetings from the EM-Labs of CIC biomaGUNE, Marco Mvller
}
}
} ----------------------------------------------------------------
} Marco Mvller
} Platform Manager - Electron Microscopy
}
} CIC biomaGUNE
} Parque Tecnolsgico de San Sebastian
} Paseo Miramsn, 182. Ed. Empresarial C
} 20009 San Sebastian (Guipzzcoa)
} SPAIN
}
} Tel: +34 943 00 53 25
} mmoller-at-cicbiomagune.es
}
}
} } -----Original Message-----
} } From: Reinhard.Rachel-at-biologie.uni-regensburg.de
} } [mailto:Reinhard.Rachel-at-biologie.uni-regensburg.de]
} } Sent: Tuesday, November 15, 2016 11:54 AM
} } To: Marco Moller
} } Subject: [Microscopy] Pirani
} }
} }
} }
} }
} } ----------------------------------------------------------------------
} } -
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} } -
} } -----
} }
} } Dear Colleagues,
} } on our Edwards Scancoat Six (now about 16 yrs old), the Pirani gauge
} } is faulty and needs to be replaced (or does anybody know how to
} } repair? we would need the W wire - can it be replaced? easy? is it W?) .
} } It is an Edwards PRE10K (D02428000) - and we asked Edwards and they
} } told us that this Pirani is not available - any more. Hmm.
} } Does anybody have one for sale which is not used any more?
} } Does anybody know another type which would fit into the Scancoat Six?
} } Any help is much appreciated.
} } kind regards,
} } Reinhard Rachel
} }
} } --
} } Prof. Dr. Reinhard Rachel
} } University of Regensburg
} } Centre for EM / Anatomy
} } Faculty of Biology & Preclin. Med.
} } Universitaetsstrasse 31
} } D-93053 Regensburg - Germany
} } tel +49 941 943 -2837, -1720
} } mail reinhard.rachel-at-biologie.uni-regensburg.de
} } office: VKL 3.1.29
} }
} } Next microscopy conferences:
} } - Microscopy Conference 2017 (D-A-CH) Lausanne, CH
} } 20-25 August 2017
} } https://urldefense.proofpoint.com/v2/url?u=http-3A__www.mc2017.ch_&d=CwIBAg&c=AGbYxfJbXK67KfXyGqyv2Ejiz41FqQuZFk4A-1IxfAU&r=MAuGvnWTcVQkxORgQD0QS50ZicPM3Nw-61ygSK-LNEQ&m=FA-wvmbqt9CTVfjWXjsi2QEA_w65rF7UvcWrmrhTFks&s=AaD5S11qNvAb-C-mDBQtYtDROq3sFEZ32NS_vLlu0U4&e=
} } - 19th International Microscopy Congress, Sydney; 9-14 Sept 2018
} } https://urldefense.proofpoint.com/v2/url?u=http-3A__imc19.com_&d=CwIBAg&c=AGbYxfJbXK67KfXyGqyv2Ejiz41FqQuZFk4A-1IxfAU&r=MAuGvnWTcVQkxORgQD0QS50ZicPM3Nw-61ygSK-LNEQ&m=FA-wvmbqt9CTVfjWXjsi2QEA_w65rF7UvcWrmrhTFks&s=-jZqy72OCAQpB_QZNwyBrgbkp-5vuH5i6hCtvqvi-oM&e=
} } - next Microbiol. conferences:
} } VAAM/DGHM - Annual Conf
} } March 5-8, 2017, Wuerzburg
} }
} }
} }
} }
} }
} } ==============================Original
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From: microscopy.listserver-at-gmail.com
Date: Tue, 15 Nov 2016 15:14:58 -0600
Subject: [Microscopy] viaWWW:Director of Electron Microscopy Laboratory Position

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Email: liljequiste-at-umkc.edu Name: Erin Liljequist

Organization: University of Missouri-Kansas City School of Dentistry

Title-Subject: [Filtered] Director of Electron Microscopy Laboratory
Position

Message: Job Description
The University of Missouri-Kansas City, School of Dentistry is seeking a
Research Assistant to fill a position as Director of the Electron
Microscopy Core Laboratory. The position is full time, 100% benefit
eligible.

Minimum Qualifications
The successful candidate will have at least 3 years training in electron
microscopy (SEM, TEM). A Ph.D. or advanced training is preferred. The
candidate should show proficiency in energy dispersive spectroscopy
(EDS), have experience in biological specimen preparation for TEM and
SEM and familiarity with corresponding equipment (ultramicrotome,
knifemaker, CPD, sputter coater) and chemical and immuno-EM procedures.
Some experience with materials specimen preparation is a plus.
Additionally, the candidate should be able to demonstrate computer
literacy and an understanding of digital image processing. Excellent
skills in collaborating with multiple faculty and time management are
essential.

Application Instructions
To apply go to the following link:
http://info.umkc.edu/hr/careers/academic-positions/
Search opening ID 21649

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interest with accompanying curriculum vitae and a list of references)
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From: p.j.f.harris-at-reading.ac.uk
Date: Wed, 16 Nov 2016 02:42:41 -0600
Subject: [Microscopy] fume cupboard

Contents Retrieved from Microscopy Listserver Archives
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Dear Colleagues,

We require a recirculating (ductless) fume cupboard for preparation of EM s= pecimens using chemicals such as osmium tetroxide, glutaraldehyde, paraform= aldehyde and epoxy resins. In the past we have used a ducted cupboard, but = this is being decommissioned. Does anyone know what face velocity is needed , or can anyone recommend a suitable cupboard?

Thanks very much

Peter Harris


Dr Peter J F Harris
Manager, Electron Microscopy Laboratory
JJ Thomson Building | University of Reading | Whiteknights | Reading = | RG6 6AF | UK


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From: oshel1pe-at-cmich.edu
Date: Wed, 16 Nov 2016 12:32:40 -0600
Subject: [Microscopy] Sad news about E. Ann Ellis

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A good friend and an excellent microscopist. She will be missed.

Phil

Philip Oshel
Microscopy Facility Supervisor
Biology Department
1304 Biosciences
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576



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From: microscopy.listserver-at-gmail.com
Date: Thu, 17 Nov 2016 04:18:15 -0600
Subject: [Microscopy] viaWWW:In Memoriam of Dr. Elizabeth Ann Ellis

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Email: trc-at-tousimis.com Name: TOUSIMIS

Organization: TOUSIMIS
Title-Subject: [Filtered] In Memoriam of Dr. Elizabeth Ann Ellis

Message: June 5, 1948 - October 29, 2016

Life Legacy

Dr. Elizabeth Ann Ellis (Ann) passed away peacefully at home Saturday
evening, October 29, under the care of Gentiva Hospice. The family is
grateful for the care that Gentiva and her “wonderful angels” from Cairo
(Mary, Rutha, and Shirley) provided in her final weeks. Ann was the
daughter of Horace W. Ellis and Vivian G. Ellis. Ann was a member of the
1966 graduating class of Thomas County Central High School. Her higher
education degrees include a Bachelor’s degree from the University of
Georgia, a Master’s degree from the University of North Carolina at
Chapel Hill, and a Doctorate from the University of Florida. While
studying for her Ph.D., she did research and publication with many
ophthalmologists and endocrinologists. As a member of the Southeastern
Microscopy Society and Microscopy Society of America, she received
numerous awards as a distinguished electron microscopist and outstanding
technologist. Dr. Ellis taught electron microscopy research techniques,
and did research and published for Texas A&M for 10 years before
retiring in 2013. While residing in College Station, Ann was a devoted
choirmember and also active as a lay leader at the University of Texas
A&M United Methodist Church. Upon returning to Thomasville, Ann became a
member of Morningside United Methodist, and also the United Daughters of
the Confederacy. Survivors include her mother, Vivian Ellis, her sister,
Pamela Ellis, and several cousins.

You will be missed by all that were fortunate enough to get to know you.
RIP Ann.

tousimis®




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From: stefan.diller-at-t-online.de
Date: Fri, 18 Nov 2016 10:12:22 -0600
Subject: [Microscopy] IDE MK3 Magnetic Field Cancellation System manual needed

Contents Retrieved from Microscopy Listserver Archives
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Dear All,

anybody out there who can send me a PDF copy of the user / installation manual of the IDE MK3 Magnetic Field Cancellation System?


Best,

Stefan


--


-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.nanoflight.info
www.stefan-diller.com
www.electronmicroscopy.info
www.elektronenmikroskopie.info
www.zwillingsprojekt.de
www.assisi.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------


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11, 22 -- From: Stefan Diller {stefan.diller-at-t-online.de}
11, 22 -- Subject: IDE MK3 Magnetic Field Cancellation System manual needed
11, 22 -- Message-ID: {1aec2f2e-4537-32f4-01de-6df8e046c43a-at-t-online.de}
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From: stefan.diller-at-t-online.de
Date: Fri, 18 Nov 2016 10:16:29 -0600
Subject: [Microscopy] FOUND: no more need.... IDE MK3 Magnetic Field Cancellation System

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,

I had been too fast and used the wrong search phrase. Finally I found a PDF in the WWW.

http://www.kotikone.fi/Jukka.Harju/Jukka/HTML/IDE%20feedforward%20users%20manual%20MK3%20MSR%20V317.pdf

No more need for the manual.

Sorry for the posting.


Stefan

-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.nanoflight.info
www.stefan-diller.com
www.electronmicroscopy.info
www.elektronenmikroskopie.info
www.zwillingsprojekt.de
www.assisi.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------

Am 18.11.16 um 17:13 schrieb Stefan Diller:
} Dear All,
}
} anybody out there who can send me a PDF copy of the user / installation manual of the IDE MK3 Magnetic Field Cancellation System?
}
}
} Best,
}
} Stefan
}
}


==============================Original Headers==============================
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From: wsalmon-at-wi.mit.edu
Date: Mon, 21 Nov 2016 13:20:08 -0600
Subject: [Microscopy] Analytical and Quantitative Light Microscopy 2017

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues,

We are excited to announce this year's run of Analytical and Quantitative Light Microscopy, held at the Marine Biological Laboratory, Woods Hole, MA, USA. The course will run May 3 - May 12, 2017.

AQLM is a comprehensive and intensive course in light microscopy for researchers in biology, medicine, and material sciences. This course provides a systematic and in-depth examination of the theory of image formation and application of digital methods for exploring subtle interactions between light and the specimen. This course emphasizes the quantitative issues that are critical to the proper interpretation of images obtained with modern wide-field, confocal microscopes and new emerging technologies.

Laboratory exercises, demonstrations, and discussions include:
- geometrical and physical optics of microscope image formation including Abbe's theory of the microscope and Fourier optics;
- phase contrast, polarization and interference microscopy;
- fluorescence microscopy, quantification of fluorescence, and fluorescent proteins;
- principles and application of digital imaging and quantitative digital image deconvolution;
- digital image processing and object identification and tracking;
- live cell and ratiometric imaging for FRET and ion concentration imaging;
- confocal microscopy and specialized methods such as TIRF and FLIM; and
- new advances in light microscopy such as FCS, PALM, STORM, SIM and Light Sheet.

The course web site is at http://www.mbl.edu/aqlm.

Within the course, we often refer to AQLM as "Microscopy Boot Camp". We cover every topic with a lecture and an in-depth hands-on lab. It's intense, it's hardcore, and it's really fun.

Why not join us for AQLM (or recommend one of your lab members or core facility users)? You'll never forget it.

Applications due January 27, 2017.

Directors: Justin Taraska (National Institutes of Health), Jagesh Shah (Harvard Medical School)
Course Laboratory Director: Wendy Salmon (Whitehead Institute/MIT)

Cheers,
Jagesh, Justin and Wendy

Visit our Facebook page: http://www.facebook.com/aqlmcourse
Follow us on Twitter: -at-BeadsinMay



~~~~~~~~~~~~~~~~~~~~~~~
Wendy Salmon
Light Microscopy Specialist
Whitehead Institute for Biomedical Research
W.M. Keck Imaging Facility
9 Cambridge Center, Rm 447
Cambridge, MA 02142
c: 617-429-0158
e: wsalmon-at-wi.mit.edu
w: http://staffa.wi.mit.edu/microscopy/


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From: microscopy.listserver-at-gmail.com
Date: Tue, 22 Nov 2016 14:10:43 -0600
Subject: [Microscopy] viaWWW:JEOL TEM 1011 Manual Needed

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Title-Subject: [Filtered] JEOL TEM 1011

Message: I have to use the above and would like to see a manual.

Can't find one online so can someone help?

Regards, Andrew

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From: andrew.johnson-at-uwa.edu.au
Date: Tue, 22 Nov 2016 18:46:08 -0600
Subject: [Microscopy] JEOL TEM 1011 instruction

Contents Retrieved from Microscopy Listserver Archives
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Organization: University of Western Australia

Title-Subject: [Filtered] JEOL TEM 1011

Message: I have to use the above and would like to see a manual.

Can't find one online so can someone help?

Regards, Andrew


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From: wij.muss-at-aon.at
Date: Tue, 22 Nov 2016 19:42:52 -0600
Subject: [Microscopy] Re: JEOL TEM 1011 Manual Needed

Contents Retrieved from Microscopy Listserver Archives
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Hi, Andrew,
unfortunately I don't have an official JEOL Manual for the/ their TEM 1011
[personally I have only parts of the JEOL TEM 1010 manuals] but - in case
nobody else will offer you a pdf,
at least I would like to point you to:

GEORGIA ELECTRON MICROSCOPY
JEOL JEM1011 [jeol_jem1011]

The JEOL JEM1011 (JEOL, Inc., Peabody, MA), a 100 kV TEM, is configured for
high contrast imaging. It is equipped with an AMT side-mounted digital
camera with 2kx2k resolution.
Click here* for a basic JEOL 1011 TEM instruction sheet.
Advanced Microscopy Techniques, Corp. Woburn, MA
*
http://web8.ovpr.uga.edu/gem/wp-content/uploads/sites/4/2016/02/JEOL-1011-Gu
ide.pdf


Cf. also:
http://www.jeolusa.com/RESOURCES/Electron-Optics/Documents-Downloads?EntryId
=560

But I am sure that at http://www.jeol.co.jp/au/ you'll find relevant
Contact Data to perhaps apply for a manual you're in need:
JEOL (AUSTRALASIA) PTY. LTD.
SALES & SERVICE

Suite 1, Level 2, 18 Aquatic Drive
Frenchs Forest NSW 2086
Australia
TEL: 02-9451-3855
FAX: 02-9451-3822
Email: info-at-jeol.com.au

Disclaimer: no affiliation, no financial interest.
============================================================================
=======


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I have to use the above and would like to see a manual.
Can't find one online so can someone help?

Regards, Andrew

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From: petereaton-at-hotmail.com
Date: Mon, 28 Nov 2016 05:39:28 -0600
Subject: [Microscopy] AFM Training 2017

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Dear All,
Our AFM training course is taking place again in beautiful Porto, Portugal between 10th and the 13th April 2017. This is the fifth edition of the course, which contains a mixture, of background, tips and tricks, sample prep., hands-on instrument training, and data analysis. The focus of the course is always on the practical aspects of AFM. As usual, we will also have some external experts come in to talk about advanced topics in materials and biological areas.
For more details of the course, visit: afmhelp.com/course
Best Regards,
Peter Eaton

____________________________________________________________________________
Atomic Force Microscopy
Dr Peter Eaton and Dr Paul West
Available through all good bookshops, or direct from Oxford University Press at:
http://ukcatalogue.oup.com/product/9780199570454.do http://afmhelp.com



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From: microscopy.listserver-at-gmail.com
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Subject: [Microscopy] viaWWW:Grid Placement in Negatively Stained Formvar-Carbon Mounted

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Email: vakimler-at-oakland.edu Name: Vickie Anna Kimler

Organization: Ocular Structure and Imaging Facility - Eye Research Institute

Title-Subject: [Filtered] Grid Placement in Negatively Stained Formvar-Carbon Mounted Biological Samples

Message: When the grid with a protein polymer is in the scope, should it be placed filament side up,
or filament side down for the sharpest digital pictures? The samples are small biopolymers in
different conformations. They are negatively stained with ethanolic 2% UA2. Thanks, Vickie

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From: jkrupp-at-deltacollege.edu
Date: Mon, 28 Nov 2016 18:10:06 -0600
Subject: [Microscopy] Electronic Lab Notebooks?

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Any one using electronic lab notebooks?

Any comments/remarks on pros/cons.

Jon

Jonathan Krupp
ASB&T
San Joaquin Delta College
5151Pacific Ave.
Stockton, CA 95207
(209) 954-5284
jkrupp-at-deltacollege.edu


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From: benada-at-biomed.cas.cz
Date: Tue, 29 Nov 2016 01:53:58 -0600
Subject: [Microscopy] Re: viaWWW:Grid Placement in Negatively Stained

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Hello Vickie,
I was taught, a long time ago, that the sample should be "placed filament side up".
Did you consider to use carbon film grids for your samples? Negative staining on thin carbon support film should result in better signal-to-noise ratio ("sharpest digital picture").

Best regards

Oldrich

--
Oldřich Benada
Institute of Microbiology CAS, v.v.i.
Laboratory of Molecular Structure Characterization
Vídeňská 1083
142 20 Prague 4
Czech Republic

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} Title-Subject: [Filtered] Grid Placement in Negatively Stained
} Formvar-Carbon Mounted Biological Samples
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} Message: When the grid with a protein polymer is in the scope, should
} it be placed filament side up, or filament side down for the sharpest
} digital pictures? The samples are small biopolymers in different
} conformations. They are negatively stained with ethanolic 2% UA2.
} Thanks, Vickie
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From: microscopy.listserver-at-gmail.com
Date: Tue, 29 Nov 2016 06:33:21 -0600
Subject: [Microscopy] viaWWW:TEM Ion Mills

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Email: wentao0108-at-gmail.com Name: Wentao Qin

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Title-Subject: [Filtered] TEM Ion Mills

Message: Which brand of ion mill, in addition to Gatan's PIPS, for TEM sample preparation is good -
I would appreciate your input! How about "Technoorg Linda"?

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From: zaluzec-at-aaem.amc.anl.gov
Date: Tue, 29 Nov 2016 06:41:17 -0600
Subject: [Microscopy] Re: Electronic Lab Notebooks?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Jonathan

After 40+ years of paper notebooks I converted to a full electronic notebook about 4 years
ago.

I use an iPad Pro with a program called NotesPlus (Apple Store). You can type via keyboard, draw or
write using a stylus, as well as capture photos and store them all in the notebook. It can import
PDFs, and for me importantly it can export the notebook or pages to a PDF file on a server
which you than then archive and/or share with colleagues. Fits my operation mode beautiflully
as I can store the experimental note with the data all in an archive.

The program is extremely cheap { $20, but it only runs on an iPad.

Disclaimer: I have no commercial connections with either companies, but
wish I did.

Nestor
Your Friendly Neighborhood SysOp


} On Nov 28, 2016, at 6:10 PM CST, jkrupp-at-deltacollege.edu wrote:
}
}
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}
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} Any one using electronic lab notebooks?
}
} Any comments/remarks on pros/cons.
}
} Jon
}
} Jonathan Krupp
} ASB&T
} San Joaquin Delta College
} 5151Pacific Ave.
} Stockton, CA 95207
} (209) 954-5284
} jkrupp-at-deltacollege.edu
}
}

===========================================
Dr. Nestor J. Zaluzec
Argonne National Laboratory
Photon Sciences Division
9700 S. Cass Ave
Bldg 212 / A-143
Argonne, Illinois 60439 USA
Email: Zaluzec-at-aaem.amc.anl.gov


Tel: 530-NES-TORZ (530-637-8679) has Voice Mail
Lab: 630-252-7901
Fax: 630-252-4798

iChat: Zaluzec-at-AIM.com
Skype: Zaluzec
Polycom: 146.139.72.119
TPM: http://tpm.amc.anl.gov


Senior Scientist - Argonne National Laboratory
Fellow of the Microscopy Society of America
Senior Fellow the Computational Institute - University of Chicago
E.P. Wigner Fellow - Oak Ridge National Laboratory
Past President Microscopy Society of America
Adjunct Professor of Physics - Northern Illinois University &
the University of Illinois at Chicago
Visiting Professor of Microscopy - Manchester University

===========================================
TPMLab: http://tpm.amc.anl.gov
MMSite: http://www.amc.anl.gov
===========================================

The box said ...
"This program requires Win 95/98/NT or better..."
So I bought a Mac !

===========================================
LLAP
=========================================+=====



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From: nicholls-at-uic.edu
Date: Tue, 29 Nov 2016 08:41:50 -0600
Subject: [Microscopy] TEM Life Science EM Tech position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The Electron Microscopy Service in the Research Resources Center at UIC is
looking for an EM Technologist. Under general supervision from the
Director of Research Service Facility (EMS), he/she will perform duties
involved in the preparation of life science specimens for observation on
scanning and transmission electron microscopes. He/She will also handle
record keeping and maintenance, with an emphasis on processing samples for
researchers, and also in the day to day running of the Electron
Microscopes.

For more information or to apply (by Monday 5th December) go to:-

https://jobs.uic.edu/job-board/job-details?jobID=72780

Regrads

--
Alan W Nicholls, PhD
Associate Director, RRC
Director Research Resource Facility - Electron Microscopy
Research Resources Center, University of Illinois at Chicago
Rm 110, 845 West Taylor St
Chicago, IL 60607
312 996 1227
nicholls-at-uic.edu


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From: Z.Zhou-at-lboro.ac.uk
Date: Wed, 30 Nov 2016 05:15:57 -0600
Subject: [Microscopy] TEM imaging Fe3O4 Magnetite particles, is it safe for the scope?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Fellow Microscopists,
Is it safe for a TEM microscope to image a holey C film dispersed with thousands of Fe3O4 magnetite particles (100nm)? The specimen is prepared in the usual way as many other nanoparticles specimens. Will the particles fly to the pole pieces? The user said the particles are iron oxide and are superparamagnetic (and have no permanent magnetisation). I don't quite understand these terms, but by diffraction I know it fits magnetite Fe3O4. If it's not safe what solutions are there to get the job done?
I would be very grateful if you can share with me your experience and comments.
Zhou

Dr Z Zhou
Research Fellow
Loughborough Materials Characterisation Centre (LMCC)
Department of Materials
Loughborough University
Leicestershire
LE11 3TU

Tel: 01509 223163
Fax: 01509 234225




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From: DusevichV-at-umkc.edu
Date: Wed, 30 Nov 2016 10:41:21 -0600
Subject: [Microscopy] Thank you

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,

Thank you very much for all the help I got in 20+ years that I am a member of the Listserver! For first 20+ years of my microscopy work I was in materials/metals field, but then found myself more and more drifting toward biomedical research. Surprisingly my transition was rather smooth. I believe it was mostly because of the great help I was getting from you, dear Listers.

Now I am retiring, but hope my experience could be helpful for somebody. I do not unsubscribe.

Thank you again,

Vladimir

P.S. In the last few years I mostly stopped replaying on questions posted on Listserver. I got tired (or I did not have time) to clean my mailbox from dozens of "out of office" messages. Now I will have more time, of course. But please, do not make this place usable only for retirees, who do have spare time. Please, find a way not to send these horrible "out of office" messages to Listserver.

P.P.S. And special thanks to Nestor.

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Director
(816) 235-2072
371 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784



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From: Peter.Eschbach-at-oregonstate.edu
Date: Wed, 30 Nov 2016 11:44:55 -0600
Subject: [Microscopy] Re: TEM imaging Fe3O4 Magnetite particles, is it safe

Contents Retrieved from Microscopy Listserver Archives
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Zhou:


I know its alarming, but yes, it is in general safe. I have looked at magnetite nanoparticles at both HP and at OSU in a JEOL 2500 and Titan TEM respectively without serious issues. They key is they need to be nanoparticles. I believe Steve Chapman and Protrain wrote a nice reply to a similar question 4 or 5 years ago. In that reply Steve mentioned that the Van der Waals force is so large on a nanoparticle they really stick well to the carbon film! I agree, I have seldom seen one fly up. However, as a precaution, I blow a duster can or dry nitrogen gas over my grids before putting any nanoparticles in the TEM (suggestion from Debby Sherman of Purdue). This works very well to dislodge any nanoparticles that are not well adhered. Then as you magnify up on the nanoparticles, halt the analysis if they begin to move!

Pete Eschbach
Oregon State University EM Facility
541 737 5645

On 11/30/16, 3:38 AM, "Z.Zhou-at-lboro.ac.uk" {Z.Zhou-at-lboro.ac.uk} wrote:




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Dear Fellow Microscopists,
Is it safe for a TEM microscope to image a holey C film dispersed with thousands of Fe3O4 magnetite particles (100nm)? The specimen is prepared in the usual way as many other nanoparticles specimens. Will the particles fly to the pole pieces? The user said the particles are iron oxide and are superparamagnetic (and have no permanent magnetisation). I don't quite understand these terms, but by diffraction I know it fits magnetite Fe3O4. If it's not safe what solutions are there to get the job done?
I would be very grateful if you can share with me your experience and comments.
Zhou

Dr Z Zhou
Research Fellow
Loughborough Materials Characterisation Centre (LMCC)
Department of Materials
Loughborough University
Leicestershire
LE11 3TU

Tel: 01509 223163
Fax: 01509 234225




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8, 42 -- Subject: Re: [Microscopy] TEM imaging Fe3O4 Magnetite particles, is it safe
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From: lamiller-at-illinois.edu
Date: Wed, 30 Nov 2016 14:15:30 -0600
Subject: [Microscopy] Winners of the MRL Student and Post Doc Presentation Competition

Contents Retrieved from Microscopy Listserver Archives
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MRL is proud to announce the winners of our Fall Conference Competition!

http://mrl.illinois.edu/2016-biological-conference-presentation-winners


Post Doc First Place: Dr Bhushan Mahadik - "A Predictive Computational Model of Hematopoietic Stem Cell Differentiation Kinetics in Culture”

Post Doc Second Place: Dr Pilgyu Kang - "Flexible and Wearable Optoelectronic Sensors for BiomedicalTechnologies: Crumpled Graphene Stretchable Photodetector”



Student First Place: Fatemeh Ostadhossein - "Antimicrobial and Diagnostic Feasibility of Inherently Therapeutic Nano-Hafnium for Periodontal Disease”

Student Second Place: Aaro Schwartz-Duval - "Towards a Cell-level Personalization of Nanomedicine: Pathology Dependent In Vitro Reduction of Gold Nanoparticles by Action of Mammalian Cells."



The MRL Fall Student/Post Doc Presentation is open to all in Academia, not just Illinois. Plans for a possible 2 Category Competition next year, one day biological, the second Material.

Stay tuned in the spring for announcements of this opportunity!


Lou Ann




{ { { { { { { { { { { { { { { { {} } } } } } } } } } } } } } } }
Lou Ann Miller
Biological Electron Microscopy
Frederick Seitz Materials Research Laboratory
Lab Room 125 Office 374 MRL
104 South Goodwin Ave
Urbana, IL 61801
Campus mail code: MC-230
Hours: 7am-3:30 pm

217-244-1567
lamiller-at-illinois.edu
http://mrl.illinois.edu



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From: colijn.1-at-osu.edu
Date: Wed, 30 Nov 2016 14:46:40 -0600
Subject: [Microscopy] carbon film replica trouble

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

HI all,

I am trying to make carbon film replicas of particulates on a surface.
After lifting the particles off the surface with standard cellulose
acetate replicating tape, I carbon coat the tape and use a condensation
washer to wash away the tape.

So far, I haven't been able to maintain an intact carbon film through
the process even after going to 80nm thick films. The only major
difference between my current procedure and what I did many years ago is
that I'm using a turbo-pumped thread coater rather than a
diffusion-pumped carbon arc deposition unit. Both systems are pulling
down to about the same pressure before the deposition.

Is there a difference in strength between a carbon film from thread and
one from a carbon arc? There is obviously a significant amount of
stress generated since the cellulose acetate swells as it begins to
dissolve.

Any suggestions?

Thanks in advance,
Henk


--------------------

Hendrik O. Colijn
Center for Electron Microscopy and AnalysiS
The Ohio State University
1305 Kinnear Rd, Suite 100, Columbus, OH 43212

colijn.1-at-osu.edu 614/643-3458
cemas.osu.edu

"Time is that quality of nature which keeps things from happening all at
once." (Ray Cummings - 1922)
Lately it doesn't seem to be working.



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13, 121 -- Subject: carbon film replica trouble
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From: dcristofori-at-unive.it
Date: Thu, 1 Dec 2016 05:52:55 -0600
Subject: [Microscopy] diamond wire saw with shorter wire

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,
has anyone experience with replacing the diamond wire in a wire saw?
Yesterday my wire broke, but not far from one end. Is it possibile to
use a shorter wire than the replacement provided by sellers? My idea was
to re-use the longest part of the broken wire, but I wonder if the wire
motor needs a the full lenght wire to work correctly.
Any comment will be appreciated.
Thanks

Davide

~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~
Davide Cristofori

direct phone = 041.234.67.26
e-mail = dcristofori[at]unive[dot]it

Centro di Microscopia Elettronica "Giovanni Stevanato" e
Dipartimento di Sc. Molecolari e Nanosistemi
Universit Ca' Foscari Venezia

Campus Scientifico, Edificio ETA
Via Torino 155 I-30172 Mestre (VE) Italy
~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~

==============================Original Headers==============================
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From: microscopy.listserver-at-gmail.com
Date: Thu, 1 Dec 2016 06:00:48 -0600
Subject: [Microscopy] viaWWW:JEOL 2100F Panel subsystem error

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Email: abdulkhaliq.khan-at-umanitoba.ca Name: Abdul Khan

Organization: University of Manitoba

Title-Subject: [Filtered] JEOL 2100F Panel subsystem error

Message: Dear all,

I have a strange issue with my JEOL 2100F. The panel subsystem and gonio subsystem on status Monitor
show error message intermittently. During error message both Left and Right panels do not work.The
error message goes away itself after about 10 minutes and then comes back again after about 10-12
minutes. This keeps on happening all the time. The system is not on service contract. Any
suggestions from the microscopy community or from Ex service guys are welcome.

Thank You

Abdul Khan
Electron Microscopy Facility Manager
Department of Mechanical Engineering
University of Manitoba, Canada
Ph; 2043337881

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From: microscopy.listserver-at-gmail.com
Date: Thu, 1 Dec 2016 06:51:58 -0600
Subject: [Microscopy] viaWWW: Biological TEM workshop March 8-10, 2017

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Email: jpshield-at-uga.edu Name: John Shields

Organization: University of Georgia

Title-Subject: [Filtered] Biological TEM workshop

Message: March 8-10, 2017, 8am-5pm each day.

This intensive, three-day workshop will provide a practical and basic theoretical introduction to
Transmission Electron Microscopy and biological sample preparation techniques. Each day will consist
of lecture, discussion and hands-on training led by GEM staff.

What: Anyone requiring training on TEM and biological sample preparation. The workshop will be
limited to 6 participants based on the availability of equipment.

Cost: UGA: $600 per participant, Non-Profit Institutions: $800 per participant, For-Profit
participants: $1200. This fee includes supplies, chemicals, hands-on beam time, personalized
training by GEM staff, and completion certificate. Lunch is provided each day.

Where: GEM laboratories, Barrow Hall, UGA
Registration: Contact John Shields (jpshield-at-uga.edu) for more information and to sign up.
Registration requires iLab account.

Instructors:

John Shields, Ph.D. – Dr. Shields is the Managing Director of GEM. He has over 25 years experience
with electron microscopy, analysis, and confocal microscopy in various disciplines. While his main
work is with biological samples, he has extensive experience with geology, food science, textiles
and archaeology.

Mary Ard – Ms Ard has over 30 years experience in electron microscopy. She is also a board-certified
Histotechnician. Ms Ard works primarily with animal pathologens for Veterinary Pathology, but has a
wide range of experience with cell biology and microbiological specimens.

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From: protrain-at-emcourses.com
Date: Thu, 1 Dec 2016 10:01:52 -0600
Subject: [Microscopy] TEM imaging Fe3O4 Magnetite particles, is it safe

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Hi

Adding to the nice comments that Peter made, you are able to reduce the
level of magnetic interference with any "uncomfortable" material, by simply
extending the objective lens focal length through adjusting your eucentric
stage to its lowest position. Cranking the z' so that you are turning your
focus controls anticlockwise will reduce the active objective lens current.
The magnification will drop, as will the level of resolution achievable.
For those who want a little more resolution the trick is to take the z' the
other way, shortening the focal length, with the increase in the operating
lens current serving to reduce the aberrations and increase resolving power.

Enjoy

Steve

Steve Chapman FRMS
Protrain for Consultancy and Training in Electron Microscopy
+44 (0)7711 606967
web www.emcourses.com

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Zhou:


I know its alarming, but yes, it is in general safe. I have looked at
magnetite nanoparticles at both HP and at OSU in a JEOL 2500 and Titan TEM
respectively without serious issues. They key is they need to be
nanoparticles. I believe Steve Chapman and Protrain wrote a nice reply to a
similar question 4 or 5 years ago. In that reply Steve mentioned that the
Van der Waals force is so large on a nanoparticle they really stick well to
the carbon film! I agree, I have seldom seen one fly up. However, as a
precaution, I blow a duster can or dry nitrogen gas over my grids before
putting any nanoparticles in the TEM (suggestion from Debby Sherman of
Purdue). This works very well to dislodge any nanoparticles that are not
well adhered. Then as you magnify up on the nanoparticles, halt the
analysis if they begin to move!

Pete Eschbach
Oregon State University EM Facility
541 737 5645

On 11/30/16, 3:38 AM, "Z.Zhou-at-lboro.ac.uk" {Z.Zhou-at-lboro.ac.uk} wrote:





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Dear Fellow Microscopists,
Is it safe for a TEM microscope to image a holey C film dispersed with
thousands of Fe3O4 magnetite particles (100nm)? The specimen is prepared in
the usual way as many other nanoparticles specimens. Will the particles fly
to the pole pieces? The user said the particles are iron oxide and are
superparamagnetic (and have no permanent magnetisation). I don't quite
understand these terms, but by diffraction I know it fits magnetite Fe3O4.
If it's not safe what solutions are there to get the job done?
I would be very grateful if you can share with me your experience and
comments.
Zhou

Dr Z Zhou
Research Fellow
Loughborough Materials Characterisation Centre (LMCC)
Department of Materials
Loughborough University
Leicestershire
LE11 3TU

Tel: 01509 223163
Fax: 01509 234225




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From: microscopy.listserver-at-gmail.com
Date: Sat, 3 Dec 2016 11:50:27 -0600
Subject: [Microscopy] viaWWW:Gas filled storage container

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Email: gary-at-microtechnics.com Name: Dr Gary Gaugler

Organization: Microtechnics

Title-Subject: [Filtered] Gas filled storage container

Message: Hi All:

I'm looking for a smallish storage container for SEM tubes and mounted specimens that I can
fill/purge with gas. This is opposite of a vacuum container. Something less than a cubic foot would
be a good size.

Any ideas or sources?

gary g.


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From: vray-at-partbeamsystech.com
Date: Sat, 3 Dec 2016 14:45:23 -0600
Subject: [Microscopy] Re: viaWWW:Gas filled storage container

Contents Retrieved from Microscopy Listserver Archives
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Hi Gary,

Small gas-purged box is very easy and quick to make.

Pick "airtight" or "weather resistant" storage box of your liking in
your local hardware store or online. Make sure box has a breather valve
to discharge excess pressure. Pick a fitting or quick-disconnect and
some 1/4" or 6mm tubing at the same place. Drill a hole in the box,
attach tube through fitting or quick-disconnect, and connect to the
purge gas supply.

Most of airtight boxes would come with a breather valve already
installed, but if not then you can get a valve on E*Bay or order here:
http://www.agmcontainer.com/breather_valves

Happy purging!
Valery :)

Valery Ray
www.linkedin.com/in/valeryray
==============================
PBS&T, MEO Engineering Company
290 Broadway, Suite 298
Methuen, MA 01844, USA
Phone: +1-978-305-0479 - leave a message
Mobie: +1-978-305-0479 - leave a message
E-mail: vray-at-partbeamsystech.com
Web: www.partbeamsystech.com
Web: www.freudlabs.com

On 12/3/2016 12:52 PM, microscopy.listserver-at-gmail.com wrote:
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} Title-Subject: [Filtered] Gas filled storage container
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}
} I'm looking for a smallish storage container for SEM tubes and mounted specimens that I can
} fill/purge with gas. This is opposite of a vacuum container. Something less than a cubic foot would
} be a good size.
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From: colijn.1-at-osu.edu
Date: Sat, 3 Dec 2016 18:50:43 -0600
Subject: [Microscopy] viaWWW:Gas filled storage container

Contents Retrieved from Microscopy Listserver Archives
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Hi Gary,

I repurposed an old glass desiccator that had a pump-out port in the lid
as a low-O2 storage unit. Basically, I stuck a styrofoam coffee cup
inside and filled it with LN2 and left the pumping port open to vent the
boil-off. I figure that the N2 is generally going to be colder than the
residual atmosphere and force the warmer air out the port. When the LN2
has evaporated, I just close the valve.

We had some people put SEM mounts that were in the air-tight storage
tubes in the desiccator. I'm not sure how much good that did!

Cheers,
Henk

--------------------

Hendrik O. Colijn
Center for Electron Microscopy and AnalysiS
The Ohio State University
1305 Kinnear Rd, Suite 100, Columbus, OH 43212

colijn.1-at-osu.edu 614/643-3458
cemas.osu.edu {about:cemas.osu.edu}

"Time is that quality of nature which keeps things from happening all at
once." (Ray Cummings - 1922)
Lately it doesn't seem to be working.



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From: lists-at-nexperion.net
Date: Sun, 4 Dec 2016 15:32:22 -0600
Subject: [Microscopy] Advanced Workshop on Cryo-Electron Tomography, Vienna, Austria, May

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues,

The Electron Microscopy Facility of the Vienna Biocenter Core Facilities (Vienna, Austria) and Nex­pe­rion - Solutions for Electron Microscopy (Vienna, Austria) are pleased to announce a jointly organised international

Advanced Workshop on Cryo-Electron Tomography
Vienna, Austria, Europe
May 6/7, 2017: Optional Pre-Course
May 8–12, 2017: Workshop

for students, post-doctoral staff, scientists, and group leaders from academia and industry.

While this workshop targets an advanced audience wit pre-existing knowledge in electron tomography, data processing and/or Cryo-TEM, a weekend pre-course will be offered for less experienced participants to catch-up.

The main part of the workshop will to cover

Immersion freezing for cryo-electron tomography
High pressure freezing and CEMOVIS
Cryo-CLEM
Low-dose data collection with SerialEM
Direct electron detectors
Cryo-STEM tomography
Processing of low-dose tilt series with IMOD
Modelling and interpretation of cryo-electron tomography data
Subtomogram averaging with PEET

Prospective Instructors include

Dr. Mikhail Eltsov, Buchmann Institute for Molecular Life Sciences, Goethe University Frankfurt, Germany
Dr. Thomas Heuser, Vienna Biocenter Core Facilities, Vienna, Austria
Dr. Johanna Höög, University of Gothenburg, Sweden
Dr. David Mastronarde, University of Colorado, Boulder, United States
Dr. Reinhard Rachel, University of Regensburg, Germany
Dr. Guenter Resch, Nexperion - Solutions for Electron Microscopy, Vienna, Austria
Dr. Sharon Grayer Wolf, Weizmann Institute of Science, Rehovot, Israel

For further details about venue and on-site infrastructure, the registration procedure (deadline: January 31, 2017), and fees, please visit

https://www.nexperion.net/cryotomo2017

We are looking forward to meeting you in Vienna,

Guenter Resch
Thomas Heuser

--
Dr. Guenter Resch
Nexperion e.U. - Solutions for Electron Microscopy - www.nexperion.net
Mattiellistrasse 3/17, 1040 Vienna, Austria - Phone +43 664 94 17 210
Registered at Commercial Court Vienna, FN 397677w - VAT Reg. ATU67962234


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8, 22 -- From: Guenter Resch {lists-at-nexperion.net}
8, 22 -- To: Microscopy-at-microscopy.com
8, 22 -- Subject: Advanced Workshop on Cryo-Electron Tomography, Vienna, Austria, May
8, 22 -- 2017
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From: richard.ross-at-allisontransmission.com
Date: Mon, 5 Dec 2016 09:55:01 -0600
Subject: [Microscopy] viaWWW:Gas filled storage container

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Take a look at a gas-ported desiccator. I have no affiliation with any supplier.

https://www.belart.com/suggested-search/product/desiccators/gas-ported.html



-----Original Message-----
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Sent: Saturday, December 03, 2016 1:12 PM
To: Richard A. Ross

X-from: gary-at-microtechnics.com

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Email: gary-at-microtechnics.com Name: Dr Gary Gaugler

Organization: Microtechnics

Title-Subject: [Filtered] Gas filled storage container

Message: Hi All:

I'm looking for a smallish storage container for SEM tubes and mounted specimens that I can fill/purge with gas. This is opposite of a vacuum container. Something less than a cubic foot would be a good size.

Any ideas or sources?

gary g.


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From: Philipp.POEML-at-ec.europa.eu
Date: Wed, 7 Dec 2016 08:16:25 -0600
Subject: [Microscopy] EMAS 2017 and IUMAS-7 in Konstanz, Germany, May 7-11 2017

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues,

(apologies for cross-posting)

If you haven't already done so, please consider joining us for EMAS 2017 - 15th European Workshop on Modern Developments and Applications in Microbeam Analysis and IUMAS-7 Meeting at the Bodensee in Konstanz, Germany from May 7th to 11th 2017.

Early bird registration for this event ends on March 15th 2017. The deadline for abstract submissions is February 28th 2017.

All details and on-line registration/abstract submission are available at:

http://www.microbeamanalysis.eu/

Best wishes
Philipp





==============================Original Headers==============================
11, 29 -- From Philipp.POEML-at-ec.europa.eu Wed Dec 7 08:16:16 2016
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11, 29 -- From: {Philipp.POEML-at-ec.europa.eu}
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11, 29 -- Subject: EMAS 2017 and IUMAS-7 in Konstanz, Germany, May 7-11 2017
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From: Angel.Paredes-at-fda.hhs.gov
Date: Wed, 7 Dec 2016 12:52:44 -0600
Subject: [Microscopy] Position available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

DEPARTMENT OF HEALTH & HUMAN SERVICES
Food and Drug Administration

Electron Microscopist
Nanotechnology Core Facility
National Center for Toxicological Research

The U.S. Department of Health and Human Services, Food and Drug Administration (FDA), National Center for Toxicological Research (NCTR), Nanotechnology Core Facility (NanoCore) is seeking to hire an experienced and motivated electron microscopist. The NanoCore has several advanced Transmission Electron Microscopes (TEM) and Scanning Electron Microscopes (SEM) to characterize nanomaterial and detect nanomaterials in cells and tissues, and analyze the structure of cells/tissues at the electron microscopic level. The equipment include 120 and 200 kV TEM, low voltage (25 kV) TEM, high resolution FE-SEM, and FE-SEM equipped with ultramicrotome for serial block face scanning electron microscopy (SBF-SEM). Applicants must have experience and proficiency in classical electron microscopy specimen preparation techniques including tissue fixation, dehydration, infiltration, embedding and ultramicrotomy. Experience with nanomaterial imaging, image analysis, electron diffraction, tomography, and energy-dispersive X-ray spectroscopy is desirable. Candidates are expected to have an interest and ability to learn and apply new techniques in order to become proficient in the cutting edge technologies employed in our facility. Additional duties will include organizing and maintaining the electron microscopy laboratory, ordering supplies, maintaining records and possess excellent written and oral communicating skills.



Job Qualifications:
. Must have and demonstrate experience and proficiency in electron microscopy
. Must possess a Bachelor or Master's degree in either material science, chemistry, or any biological field.
. Experience with nanotechnology and or image analysis is desirable.

Salary for this position will be commensurate with experience. Interested individuals should send their resume to Dr. Anil K. Patri at the following address:

Anil K. Patri, Ph.D.,
Director, NCTR-ORA Nanocore HFT-30, 3900 NCTR Road, Jefferson, AR 72079 anil.patri-at-fda.hhs.gov

Applications will be accepted until the position is filled. FDA is an Equal
Opportunity Employer. FDA/NCTR is a smoke free environment.



The NCTR, located approximately 30 miles southeast of Little Rock, Arkansas, conducts FDA mission-related research that is of critical importance to the Agency in developing a scientifically sound basis for regulatory decisions. Over 150 Ph.D. scientists and 400 support scientists, on-site-contractors and administrative staff make up a dynamic group of professionals in the NCTR organization. Undergraduate and graduate students, post-doctoral fellows and visiting scientists also pursue education and research opportunities in a multi- disciplinary team atmosphere. For more information on NCTR research and training activities, visit http://www.fda.gov/NCTR.



==============================Original Headers==============================
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From: microscopy.listserver-at-gmail.com
Date: Thu, 8 Dec 2016 07:24:21 -0600
Subject: [Microscopy] viaWWW:Foundations of Electron Microscopy Short Course

Contents Retrieved from Microscopy Listserver Archives
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X-from: degraef-at-cmu.edu

This Question/Comment was submitted to the Microscopy Listserver
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Email: degraef-at-cmu.edu Name: Marc De Graef

Organization: Carnegie Mellon University

Title-Subject: [Filtered] Foundations of Electron Microscopy Short Course

Message: I would like to draw your attention to a Short Course offered early January (10-11 2017) at
Johns Hopkins University in Baltimore. The course will cover theory and simulations for imaging and
diffraction in both SEM and TEM and will be at the graduate level. Course and registration
information can be found at the following URL:
http://hemi.jhu.edu/academic-programs/short-courses/foundations-of-electron-microscopy/

Please pass this on to any graduate students or post-doctoral researchers who may be interested in
this course.

Regards,
Marc.

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From: andybarlow100-at-hotmail.com
Date: Thu, 8 Dec 2016 08:15:42 -0600
Subject: [Microscopy] Resolution calculator for Android devices

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Microscopy List,


I'd like to draw your attention to a new tool for microscopists I've developed for Android devices, "Resolution".


After entering magnification, immersion medium, lambda, and NA the App will calculate resolution (actual and theoretical), axial resolution and fluorescence brightness of your objective.

Each objective entered can be easily saved and restored.

You can select your camera, binning and additional magnification to determine if you're sampling at Nyquist frequency.

All information generated get be easily shared via e-mail or MMS with the share button.

The App is free to download and compatible with phones or tablets running Android 4.0 or above.

I hope you find it useful!

Andrew L. Barlow

Please use the link below on your Android device to install:

https://play.google.com/store/apps/details?id=com.Barlowax.resolutionfragments








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From: microscopy.listserver-at-gmail.com
Date: Fri, 9 Dec 2016 07:46:37 -0600
Subject: [Microscopy] viaWWW:Announcement of CIASEM 2017

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Email: ciasem2017-at-imre.uh.cu Name: Carlos Alberto Lariot

Organization: Cuban Society of Microscopy

Title-Subject: [Filtered] Announcement of CIASEM 2017

Message: XIV Interamerican Congress on Microscopy SECOND ANNOUNCEMENT
The Cuban Society of Microscopy and the Organizing Committee of the XIV Interamerican Congress on
Microscopy (CIASEM 2017) announces some important aspects of this conference, which will be held
next year, from September 25 to 29 in Varadero, the most beautiful beach of Cuba. CIASEM 2017 will
include eight symposia in Material Science, seven in Life Sciences, one in Advances in
Instrumentation and one in Teaching of Morphological Sciences. Five Precongress courses updating on
various topics of microscopy and a micrograph contest are also scheduled. There will be a
Commercial Exhibition of companies that supply microscopes, as well as other equipment and reagents
for microscopy. A profitable interchange with their skilled specialists may represent a valuable
update for all the delegates regarding new equipment and analytical techniques. Students and early
career researchers can apply for the scholarships that the International Federation of Microscopy
(IFSM) and the Microscopy Society of America (MSA) will provide to attend CIASEM 2017. A special
package which includes a 5-night, all-inclusive accommodation at the Melia Marina Varadero Hotel 5*
(venue of this conference) with a customized assistance after arrival to Cuba at Havana or Varadero
Airport, is provided for the CIASEM 2017 delegates, and those flying by COPA Airlines will have a
20% discount. Besides the charm of the beautiful Varadero beach, and the comfortable, welcoming
Melia hotel, visitors will have the opportunity to go on excursions to several tourist attractions
of our country. In our website, www.ciasem2017.sld.cu, you can log in and find updated information
during all the organization stages of CIASEM 2017.
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From: microscopy.listserver-at-gmail.com
Date: Sat, 10 Dec 2016 09:45:06 -0600
Subject: [Microscopy] viaWWW:Postdoc in AC-STEM Immediately at ASU

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Email: jingyue.liu-at-asu.edu Name: Jingyue (Jimmy) Liu

Organization: Arizona State University

Title-Subject: [Filtered] Postdoc in AC-STEM Immediately at ASU

Message: Immediately Available
A Postdoctoral Research Associate position in aberration-corrected STEM of supported metal catalysts
is immediately available in Professor Jingyue (Jimmy) Liu's group at Arizona State University (ASU).
Responsibilities: The Postdoctoral Research Associate is responsible for characterizing a variety of
supported metal/alloy nanoparticle catalysts, analyzing and evaluating the results, correlating the
structure-performance relationships, discussing data with multi-site project team members, and
writing technical reports and manuscripts for peer reviewed journal publications.
Qualifications: A Ph.D. in chemistry, materials science and engineering, physics or a related field;
outstanding knowledge and skills in AC-STEM and associated techniques (HAADF, EDS and EELS);
extensive experience in STEM characterization of supported metal/alloy catalysts; knowledge and
experience in using modern SEM, microprobe and XRD.

To apply, please submit a single PDF document containing (1) a cover letter which includes the names
and email addresses of three references and (2) a curriculum vitae with a list of publications to
Jingyue.liu-at-asu.edu.

Note: This position is funded by an industry partner and is initially planned for two years
depending on performance and available funding. ASU will provide H-1B visa for all Postdoctoral
Research Associates.


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From: microscopy.listserver-at-gmail.com
Date: Sun, 11 Dec 2016 10:57:11 -0600
Subject: [Microscopy] viaWWW: SEM - Position changes when change sample height

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Email: 13qw9-at-queensu.ca Name: Jason

Title-Subject: [Filtered] Position changes when change sample height

Message: Hello SEM users,
I have a problem about using an FEI SEM. After I focused and linked Z, the sample position will
always change a lot when I raise or drop the sample. Basically the area in the image will go to the
opposite direction with the sample.why would this happen? Seems like this only happens in my acciu.
I don't have much knowledge about SEM theory so, any advice is appreciated.

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From: microscopy.listserver-at-gmail.com
Date: Mon, 12 Dec 2016 08:47:20 -0600
Subject: [Microscopy] viaWWW:Embedding of nerves for TEM

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Email: lenech-at-nmbu.no Name: Lene Cecilie Hermansen

Organization: Imaging Centre, NMBU

Title-Subject: [Filtered] Embedding of nerves for TEM

Message: I have tried to embed nerves in LRWhite 3 times now, but it is not successful. The last
time I washed the samples more than usual, change the diluted and 100 % embedding media more than
twice as often, and spend 5 days on the procedure. The nerves are all soft, I get an empty space
under the sample, and they are impossible to section. I thought the problem was getting rid of all
the osmium, because I embedded some without osmium, and they turned out fine.
Does anyone know what the problem could be, and how I should solve it?
Best regards Lene


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From: wtivol-at-sbcglobal.net
Date: Mon, 12 Dec 2016 18:20:38 -0600
Subject: [Microscopy] Re: viaWWW: SEM - Position changes when change sample height

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} On Dec 11, 2016, at 9:17 AM, microscopy.listserver-at-gmail.com wrote:
}
}
} X-from: 13qw9-at-queensu.ca
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at http://www.microscopy.com/MLFormMail.html
} Email: 13qw9-at-queensu.ca Name: Jason
}
} Title-Subject: [Filtered] Position changes when change sample height
}
} Message: Hello SEM users,
} I have a problem about using an FEI SEM. After I focused and linked Z, the sample position will
} always change a lot when I raise or drop the sample. Basically the area in the image will go to the
} opposite direction with the sample.why would this happen? Seems like this only happens in my acciu.
} I don't have much knowledge about SEM theory so, any advice is appreciated.
}
Dear Jason,
It seems like the beam is tilted. Have you done that alignment? I am not an expert on SEM, so maybe someone who is will also reply.
Yours,
Bill





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From: colijn.1-at-osu.edu
Date: Mon, 12 Dec 2016 18:45:30 -0600
Subject: [Microscopy] Re: viaWWW: SEM - Position changes when change

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Hi Jason, Bill, et al.,

It it sounds like there is quite a bit of movement in the image. If so,
it may be that the stage Z axis isn't parallel to the beam axis.

First determine in which direction the image is shifting. On most of
the FEI scopes I've worked with, the stage Y axis is vertical and X axis
is horizontal on the screen. If the image shift is along the vertical
(Y) axis, adjust your sample tilt several degrees + & -. One direction
should increase the shift and the other decrease it. If the shift is
along the X axis, visually examine the stage from the side and see if
you can see anything that is cocked.

I would recommend discretion when fiddling with the stage since the
bearings and other components are matched for submicron precision. This
may be worth a service call.

Good luck,
Henk

--------------------

Hendrik O. Colijn
Center for Electron Microscopy and AnalysiS
The Ohio State University
1305 Kinnear Rd, Suite 100, Columbus, OH 43212

colijn.1-at-osu.edu 614/643-3458
cemas.osu.edu {about:cemas.osu.edu}

"Time is that quality of nature which keeps things from happening all at
once." (Ray Cummings - 1922)
Lately it doesn't seem to be working.



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15, 128 -- Subject: Re: [Microscopy] Re: viaWWW: SEM - Position changes when change sample height
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From: protrain-at-emcourses.com
Date: Tue, 13 Dec 2016 04:45:36 -0600
Subject: [Microscopy] SEM - Position changes when change sample height

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Jason

When you say change or drop the specimen I assume you mean a Z position
adjustment? The "problem" that you see with a change in Z is quite normal.
The vertical motion of the stage is through a thread and the take up of any
slack in that thread results in specimen movement in X and Y directions.
With an electrically driven stage manufacturers have the option of providing
anti backlash adjustments to make X and Y movements more positive; I do not
know a manufacturer that has anti backlash compensation in the Z direction.

Steve

Steve Chapman FRMS
Protrain for Consultancy and Training in Electron Microscopy
+44 (0)7711 606967
web www.emcourses.com

----------------------------------------------------------------------------

Email: 13qw9-at-queensu.ca Name: Jason

Title-Subject: [Filtered] Position changes when change sample height

Message: Hello SEM users,
I have a problem about using an FEI SEM. After I focused and linked Z, the
sample position will always change a lot when I raise or drop the sample.
Basically the area in the image will go to the opposite direction with the
sample.why would this happen? Seems like this only happens in my acciu.
I don't have much knowledge about SEM theory so, any advice is appreciated.



==============================Original Headers==============================
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From: microscopy.listserver-at-gmail.com
Date: Mon, 19 Dec 2016 07:11:25 -0600
Subject: [Microscopy] viaWWW:particle separation on microscope slide

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Email: joseph.uknalis-at-ars.usda.gov Name: Joe Uknalis

Organization: USDA-ARS

Title-Subject: [Filtered] particle separation on microscope slide

Message: Hello folks-
I have an upcoming project to measure the particle sizes of powders/grindings.
Do folks have a ‘best practice’ on getting good separation of particles on a microscope slide?
Samples will be wet.

If anyone has a clever idea on how to do this it would be much appreciated.

Thanks

Joe


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From: microscopy.listserver-at-gmail.com
Date: Mon, 19 Dec 2016 07:12:26 -0600
Subject: [Microscopy] viaWWW: EDGE EELS Meeting in Japan

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Email: crozier-at-asu.edu Name: Peter Crozier

Organization: Arizona State University

Title-Subject: [Filtered] EDGE EELS Meeting in Japan

Message: The next International Electron Energy Loss Spectroscopy Meeting (EDGE 2017), will be held
at Okuma, Okinawa, Japan, May 14 – 19, 2017. The purpose of the meeting is to cover the latest
developments in electron energy loss spectroscopy and related techniques in the transmission
electron microscope. The event is attended by approximately 100-130 participants from around the
world in an informal setting and is aimed at promoting scientific discussions and
cross-fertilization of ideas from complementary techniques. Abstracts will be due January 22, 2017.
More information can be found in the attached flyer and at the conference website at:
http://www.nims.go.jp/EDGE2017/.

Best Regards,
Scientific and Local Host Committee Chairs Odile Stéphan, Peter Crozier and Koji Kimoto,


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From: microscopy.listserver-at-gmail.com
Date: Wed, 21 Dec 2016 17:41:08 -0600
Subject: [Microscopy] viaWWW:Job Opening - Electron Microscopy Specialist Washington

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Email: fitzp-at-wustl.edu Name: James Fitzpatrick

Organization: Washington University School of Medicine, St. Louis, MO

Title-Subject: [Filtered] Job Opening - Electron Microscopy Specialist

Message: I am hiring an Electron Microscopy Specialist for my lab, the Center for Cellular Imaging
at Washington University School of Medicine - http://wucci.wustl.edu.

Ideal candidate will have a background in Cell Biology, with thin section and freeze fracture
expertise along with high-resolution TEM analysis.
Apply to Job ID: 35418 - Research Specialist (Electron Microscopy) - Center for Cellular Imaging

https://jobs.wustl.edu

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From: microscopy.listserver-at-gmail.com
Date: Wed, 21 Dec 2016 17:42:03 -0600
Subject: [Microscopy] viaWWW: Carbon Grains on Formvar Grids

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Email: vakimler-at-oakland.edu Name: Vickie Kimler

Organization: Oakland University Eye Research Institute

Title-Subject: [Filtered] Carbon Grains on Formvar Grids

Message: What is the approximate size of the carbon grains on Formvar grids? I hava also noted that
if my objective aperture on the TEM is out, the grains seem to disappear (if that is what they are)
when I image at 60-90kx. I am guessing that the "rough", salt-and-pepper appearance on the grids is
carbon. I am using a TEM designed for biological samples. Thanks, Vickie

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From: wtivol-at-sbcglobal.net
Date: Wed, 21 Dec 2016 18:46:21 -0600
Subject: [Microscopy] Carbon Grains on Formvar Grids

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} Email: vakimler-at-oakland.edu Name: Vickie Kimler
}
} Organization: Oakland University Eye Research Institute
}
} Title-Subject: [Filtered] Carbon Grains on Formvar Grids
}
} Message: What is the approximate size of the carbon grains on Formvar grids? I hava also noted that
} if my objective aperture on the TEM is out, the grains seem to disappear (if that is what they are)
} when I image at 60-90kx. I am guessing that the "rough", salt-and-pepper appearance on the grids is
} carbon. I am using a TEM designed for biological samples. Thanks, Vickie

Dear Vickie,
Amorphous carbon has no long-range order, but as the carbon atoms are deposited on the formvar, they form graphite-like bonds. There are probably patches of these bonded carbon atoms about 1 nm across—this is just a guess. The image loses contrast when you remove the objective aperture, because all the scattered electrons contribute to the image, which is mostly phase contrast. With the aperture in place, some of the scattered electrons are removed from the image, and these are the ones scattered by smaller objects, such as carbon grains. Furthermore, the closer you are to exact focus, the lower the contrast. If you see rough grains, they are due to defocus fringes that overlap, and if you look at the Fourier transform, you will see Thon rings. You can use these to correct astigmatism, determine the resolution of the instrument, or determine the value of the defocus. It should not matter whether your scope is for biology or materials.
Yours,
Bill





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From: microscopy.listserver-at-gmail.com
Date: Thu, 22 Dec 2016 07:52:44 -0600
Subject: [Microscopy] viaWWW:CM10 vacuum problem

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Email: pveril-at-uth.gr Name: P. Berillis

Organization: University of Thessaly, Hellas

Title-Subject: [Filtered] CM10 vacuum problem

Message: Hi everybody.
I have a vacuum problem with a CM10 TEM. 4 day ago the microscope worked fine and in high
magnification (we examined some virus particles). When I finished I shut it down. Yesterday when I
switch it on the microscope stacked to the "vacuum status: start up" for hours. I can hear the PVP
working, but the pre-vacuum led is of and the ODP is cold. The monitor shows these pressure values:
P1: 38, P2: 38, P3: 0 and IGP: 0. Have anyone have some answers?
With my best regards
Panos

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From: microscopy.listserver-at-gmail.com
Date: Thu, 22 Dec 2016 07:59:00 -0600
Subject: [Microscopy] viaWWW:CM10 vacuum problem

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Pveril

If your PVP is running with out shutting down you most likely have a vacuum leak
in the backing lines.

Check the hoses from the roughing pump to the DP. Are they old and cracked?
The other place to check is the o-ring connections on the DP backing line. They
also get old (hot) and cracked. I had to replace them on my CM200 just a month
ago.

Cheers,

Nestor
Your Friendly Neighborhood SysOp


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Email: pveril-at-uth.gr Name: P. Berillis

Organization: University of Thessaly, Hellas

Title-Subject: [Filtered] CM10 vacuum problem

Message: Hi everybody.
I have a vacuum problem with a CM10 TEM. 4 day ago the microscope worked fine and in high
magnification (we examined some virus particles). When I finished I shut it down. Yesterday when I
switch it on the microscope stacked to the "vacuum status: start up" for hours. I can hear the PVP
working, but the pre-vacuum led is of and the ODP is cold. The monitor shows these pressure values:
P1: 38, P2: 38, P3: 0 and IGP: 0. Have anyone have some answers?
With my best regards
Panos

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From: dev.c0debabe-at-gmail.com
Date: Thu, 22 Dec 2016 08:03:51 -0600
Subject: [Microscopy] RJ Lee / Aspex PSEM - Low Emission Current

Contents Retrieved from Microscopy Listserver Archives
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Hi,

we have an older model (1995 or so) Aspex PSEM and are in general
familiar with the electronics in the SEM.
A few weeks ago, we noticed that the emission current started to jump
around. This continued after replacing the filament, but stopped lateron
and we are now stuck with a low emission current.

After filament alignment using the source imaging mode, we are now stuck
with no more than 23uA of emission current at 20kV, the highest bias
voltage setting and the standard 200um aperture in the liner tube.
By turning up the "filament drive" software control, the emission
current steadily increases until the 23uA at about "65%" filament drive.
Turning up the filament drive more than that does no longer increase the
emission current.

As I mentioned, we are familiar with the SEM electronics and we are
willing to take things apart and have a look for faulty components
including measurements on the PCBs.
Since the emission current started to jump around before we got the low
emission current, I would suspect that something in the high voltage
power supply failed (maybe a faulty cap or something).

Before taking apart things, do you have any suggestions of what we could
try to ramp up the emission current ?
The filament should be aligned and the bias voltage is already at its
maximum.

Are there any specific electronic components in the HVPS that are known
to cause this behavior if they fail (e.g. failing capacitors due to
their age, voltage regulators) ?

Is there any information available on the HVPS test points and the
voltages we should get there so that we can compare them to the voltages
within our system ?


Thanks,
Stefan

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From: evoptics-at-iafrica.com
Date: Thu, 22 Dec 2016 08:23:35 -0600
Subject: [Microscopy] viaWWW:CM10 vacuum problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Panos,
Edwards ODP has Oil Fill and Oil Drain connections on base of pump. Each
item has O ring seal. Area subject to very high temperature results in
deform and cracking of seals. Replace with Kalrez type O rings. Confirmation
is with Prevac Pump running, readout of P1 and P2 does not fall below 38 as
indicated.

Regards, Dave.
eV Optics cc
South Africa.

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Email: pveril-at-uth.gr Name: P. Berillis

Organization: University of Thessaly, Hellas

Title-Subject: [Filtered] CM10 vacuum problem

Message: Hi everybody.
I have a vacuum problem with a CM10 TEM. 4 day ago the microscope worked
fine and in high magnification (we examined some virus particles). When I
finished I shut it down. Yesterday when I switch it on the microscope
stacked to the "vacuum status: start up" for hours. I can hear the PVP
working, but the pre-vacuum led is of and the ODP is cold. The monitor shows
these pressure values:
P1: 38, P2: 38, P3: 0 and IGP: 0. Have anyone have some answers?
With my best regards
Panos

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From: vitalylazar-at-att.net
Date: Thu, 22 Dec 2016 11:35:31 -0600
Subject: [Microscopy] Re: viaWWW:CM10 vacuum problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

possibly vacuum leak anywhere between PVP and ODP Especially if PVP
stays ON continuously. Likely places PVP inlet hose and ODP dipstick
O-rings. The doubt is in P1/P2 = 38. Anything below 39 should not
prevent ODP from heating up.

possibly PVP is not performing, possibly just needs oil change.

possibly ODP heater is bad or has no power. The doubt is - this
shouldn't prevent P1/P2 from reaching values much lower than 38.

assuming P1/P2 readings correct (they are unlikely to be equally incorrect).

I need more details for remote diagnosis, you welcome to contact me off
list.

Vitaly Feingold
SIA
2773 Heath Lane
Duluth GA 30096
Ph. 770-232-7785
Fax 770-232-1791
www.sia-cam.com
vitaly-at-sia-cam.com

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} Title-Subject: [Filtered] CM10 vacuum problem
}
} Message: Hi everybody.
} I have a vacuum problem with a CM10 TEM. 4 day ago the microscope worked fine and in high
} magnification (we examined some virus particles). When I finished I shut it down. Yesterday when I
} switch it on the microscope stacked to the "vacuum status: start up" for hours. I can hear the PVP
} working, but the pre-vacuum led is of and the ODP is cold. The monitor shows these pressure values:
} P1: 38, P2: 38, P3: 0 and IGP: 0. Have anyone have some answers?
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From: John.Mardinly-at-asu.edu
Date: Thu, 22 Dec 2016 13:31:23 -0600
Subject: [Microscopy] Re: Carbon Grains on Formvar Grids

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Vickie, Bill;
The apparent carbon “grains” you see are purely an artifact of imaging in a microscope with a lot of spherical aberration! The Thon Rings you see in your FFT are a rotational display of the square of the modulation transfer function of the objective lens, which changes as focus changes. If all this is a mystery to you, you should attend the ASU Winter School on High-Resolution Electron Microscopy, which starts January 3rd. If you cannot do that, read the classic (and superb) text ’Experimental High-Resolution Electron Microscopy” by John Spence.

A. John Mardinly, Ph.D., P.E.
Retired Principal Materials Nanoanalysis Engineer
ASU



} On Dec 21, 2016, at 6:05 PM, wtivol-at-sbcglobal.net wrote:
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} } Email: vakimler-at-oakland.edu Name: Vickie Kimler
} }
} } Organization: Oakland University Eye Research Institute
} }
} } Title-Subject: [Filtered] Carbon Grains on Formvar Grids
} }
} } Message: What is the approximate size of the carbon grains on Formvar grids? I hava also noted that
} } if my objective aperture on the TEM is out, the grains seem to disappear (if that is what they are)
} } when I image at 60-90kx. I am guessing that the "rough", salt-and-pepper appearance on the grids is
} } carbon. I am using a TEM designed for biological samples. Thanks, Vickie
}
} Dear Vickie,
} Amorphous carbon has no long-range order, but as the carbon atoms are deposited on the formvar, they form graphite-like bonds. There are probably patches of these bonded carbon atoms about 1 nm across—this is just a guess. The image loses contrast when you remove the objective aperture, because all the scattered electrons contribute to the image, which is mostly phase contrast. With the aperture in place, some of the scattered electrons are removed from the image, and these are the ones scattered by smaller objects, such as carbon grains. Furthermore, the closer you are to exact focus, the lower the contrast. If you see rough grains, they are due to defocus fringes that overlap, and if you look at the Fourier transform, you will see Thon rings. You can use these to correct astigmatism, determine the resolution of the instrument, or determine the value of the defocus. It should not matter whether your scope is for biology or materials.
} Yours,
} Bill
}
}
}
}
}
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From: microscopy.listserver-at-gmail.com
Date: Fri, 23 Dec 2016 06:29:15 -0600
Subject: [Microscopy] viaWWW: SEM Peltier cooling stage

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Email: researchers4u-at-gmail.com Name: Sun

Organization: Shiraz university, iran

Title-Subject: [Filtered] SEM Peltier cooling stage

Message: Dear colleagues:
recently we are trying to purchase a peltier cooling stage for the SEM samples that needs to be
frozen like: milk,ice cream, fruits and generally food industry. And this stage that has been
offered to us by a middle company gets to -50 centigrade to +70 centigrade. I was wondering for our
applications is this temperature range is enough? Or should we go for other cooling stages with
-180 centigrade?

Thank you for your information and time

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From: microscopy.listserver-at-gmail.com
Date: Fri, 23 Dec 2016 06:30:40 -0600
Subject: [Microscopy] viaWWW: TEM Drierite drying columns

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Email: susan.wert-at-cchmc.org Name: Susan E. Wert, PhD

Organization: Cincinnati Children's Hospital Medical Center/Research Foundation

Title-Subject: [Filtered] TEM Drierite drying columns

Message: I have 2 unused Drierite drying columns – 2 5/8 x 11 3/8 inches, molded acrylic plastic,
with anodized aluminum caps fitted with O-ring gaskets, for working pressures up to 90 psig.
Connections for rubber tubing at top and bottom of column, at right angles to the tube. Flow rate
200 liters per hour. Still in original boxes – purchased for a Balzers freeze-fracture machine. Age
unknown, but Drierite is pink and would need to be regenerated.
Free except for cost of shipping. Otherwise would appreciate ideas for “recycling”.

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From: colijn.1-at-osu.edu
Date: Fri, 23 Dec 2016 08:40:23 -0600
Subject: [Microscopy] viaWWW: SEM Peltier cooling stage

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Hi Sun,

As you consider the cooling stage, you should think about the vapor
pressure of what you are inserting into your chamber. I assume that you
have a high-vacuum SEM and that the primary vapor you are concerned with
is water. The tables of water vapor pressure as a function of
temperature should be readily available online. You want to ensure that
the partial pressure of water is *much* below (orders of magnitude) the
chamber pressure of your scope.

Also, you need to take into account the thermal conductivity of the
sample you are examining. Remember that the temperature of the exposed
surface can be well above the cooling temperature of the stage itself.
Remember that the beam will also cause local heating of the sample. In
my TEM, I have seen ice evaporate under the beam when the stage
temperature was reading {-100C.

Cheers,
Henk

--------------------

Hendrik O. Colijn
Center for Electron Microscopy and AnalysiS
The Ohio State University
1305 Kinnear Rd, Suite 100, Columbus, OH 43212

colijn.1-at-osu.edu 614/643-3458
cemas.osu.edu

"Time is that quality of nature which keeps things from happening all at
once." (Ray Cummings - 1922)
Lately it doesn't seem to be working.



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14, 123 -- Subject: Re: [Microscopy] viaWWW: SEM Peltier cooling stage
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From: wesaia-at-iastate.edu
Date: Fri, 23 Dec 2016 13:26:01 -0600
Subject: [Microscopy] viaWWW: SEM Peltier cooling stage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

First, to all of you vendors and labs that I have no dealings with except for being on this list, and who are still dutifully going to tell me that you are out of the office for the holidays, I wish you a Merry Christmas and Happy New Year anyway. I do still wish that you would find a way to spare list-posters from your notices.

I will second Henk's comments that the beam will lead to localized increased temperatures. You will have to play around with the conditions to get what you want.

We have a Peltier stage on our Quanta. I'm not sure that ours has as much as a -50C to 70C range. I think our cooler allows 25C deviation up or down from ambient and that is relative to a temperature conditioned water bath. I am not sure you would need the -180C model.

I would ask you to consider the full process of your experiments. How will you introduce your samples? Do you have a cryo transfer system? How will you prepare your samples for examination? That could be in-situ but probably elsewhere. Do you have an SEM capable of low-vacuum or environmental mode?

Henk, you spoke of keeping the vapor pressure of the water in the sample far below the pressure of the chamber. Are you dealing with a high-vacuum-only instrument? Couldn't that still result in problems? If the partial pressure of water in your vacuum is below the vapor pressure of water at the sample temperature, the water in the sample would still sublime. Granted, if the temperature is very low, that should be a slow process. Conversely, if the partial pressure of water in your chamber is higher that the vapor pressure of water at sample temperature, then you would have frost build up on the sample through deposition. It will be a tricky business.

Warren

-----Original Message-----
X-from: colijn.1-at-osu.edu [mailto:colijn.1-at-osu.edu]
Sent: Friday, December 23, 2016 8:42 AM
To: Straszheim, Warren E [BIOTC]

Hi Sun,

As you consider the cooling stage, you should think about the vapor pressure of what you are inserting into your chamber. I assume that you have a high-vacuum SEM and that the primary vapor you are concerned with is water. The tables of water vapor pressure as a function of temperature should be readily available online. You want to ensure that the partial pressure of water is *much* below (orders of magnitude) the chamber pressure of your scope.

Also, you need to take into account the thermal conductivity of the sample you are examining. Remember that the temperature of the exposed surface can be well above the cooling temperature of the stage itself.
Remember that the beam will also cause local heating of the sample. In my TEM, I have seen ice evaporate under the beam when the stage temperature was reading {-100C.

Cheers,
Henk

--------------------

Hendrik O. Colijn
Center for Electron Microscopy and AnalysiS The Ohio State University
1305 Kinnear Rd, Suite 100, Columbus, OH 43212

colijn.1-at-osu.edu 614/643-3458
cemas.osu.edu

"Time is that quality of nature which keeps things from happening all at once." (Ray Cummings - 1922)
Lately it doesn't seem to be working.



------ Original Message ------
X-from: microscopy.listserver-at-gmail.com
To: colijn.1-at-osu.edu
Sent: 12/23/2016 7:31:48 AM


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21, 54 -- Subject: RE: [Microscopy] Re: viaWWW: SEM Peltier cooling stage
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From: FMonson-at-wcupa.edu
Date: Mon, 26 Dec 2016 13:37:28 -0600
Subject: Re: [Histonet] Coverslipping

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

This response will be obscure, because I learned my skills from instructors who learned in the early 20th century.

1. When I covered a section on a 1x3" slide, I normally used a 7/8 x 7/8" #1 cover-slip.
2. The mountant was prepared "in house" from "Dewaxed Gum Damar"
3. When I used up what I was given with my PhD, I purchased a 1 lb jar of dewaxed chunks of Gum Damar
a. De-waxed was NOT de-junked, so I dissolved the lb in a gallon of xylene so I could filter the solution to remove all of the 'other' undissolved stuff. As I recall, I performed this operation, in a hood, and did it at least twice.
b. Then, I placed a cotton plug in the gallon container that was covered with a 250ml beaker and left with an appropriate sign - "to evaporate-to-appropriate consistency." [This was determined by the rates at which drops fell from the 1/8" glass applicator.) This took at least two years until I had a liter that has now sat for almost 20 years with only one aliquot removed.
4. Gum Damar was used, (anecdotally and empirically) because it did NOT dry out if applied at a proper concentration. I have slides that I prepared in the mid-1960's, and the appearance and clarity beneath the cover-slip has not changed.
5. How was I challenged to administer this wonderful material?
a. Each applicant was advixsed to make a list of the number(s) of drops from the glass-rod applicator when the drops fell at a 'certain' rate, for each of the cover-slips that we had available in the lab - little circles to the cover-slips for 2x3" slides.
6. All the above now given as memory permits, I will give the routine when applying the cover-slip - my way. [I do not know how a modern applicator does this job, so I am unaware how it might be incorrectly done in the case of the ?!?"!?!?]
a. Taking an individually cleaned - to biochemical standards! - cover-slip, place it on a clean 90mm circle of Whatman #2 - no ash , thus, absent of nano junk - and apply the required drops for the cover-slip being used in a personalized array of droplets.
b. Bring the slide with a section out of its last 'zylene' rinse and wipe carefully on the back of the slide and around the section. When the breeze from the nearby open window has evaporated a sufficient amount of residual zylene, invert the slide and carefully - holding it at an appropriate angle - bring the slide to the mountant on the cover-slip and carefully bring the angle to zero trapping no bubbles in the process.
c. Turning the slide right-side-up , set it aside in an appropriate tray, protected from the dust in the breeze.
d. When all slide are prepared, having used 22x22mm #1 glass to cover the section on the 1x3" slide, place on each cover-slip a block of lead whose dimensions were 2-3mm less around its perimeter so as to avoid getting stuck to the cover-slip in the cases of new cover-slippers.
i. This peculiar dimensional confusion was explained as an attempt to overcome the the slide we biologists had taken into the metric system while preparing for our careers in biology in chemistry departments across the planet.]
ii. The purpose of the Pb was to minimize the mountant; for, the thickness of the section varies more than anyone knows - or, perhaps, cares.
iii. Lots of new findings have found their way into journals when the explanations of the 'data' were the thickness of the sections rather than what was claimed. Two classes of Sertoli Cells in one case based on two classes of nuclei - 'dark' and 'light.'
7. So, as you may have discerned, I have a 'Monkish' manner about things scientific.
a. Example 1: I do not ever want the mountant to dry. Rather, I would choose a mountant that on concentrating at its perimeter of the cover-slip such that it forms a seal that prevents any further drying.
b. Example 2: I have NEVER taught 'di-section.' Rather, I have made use of my knowledge of proper prefixes and dutifully taught 'dis-section.' I once did a di-section before a class - with a machete - on a short shark in order to demonstrate the difference between what the students all said they were going to do and what I was going to grade them on!
8. AN UNIMPORTANT POSTLUDE. For those of you who chuckle with me about various things, I will leave you with a mystery that I will take to my grave. Background aside, there is no way that I should have received money from NIH (in the mid 1990's) to answer a simple histological question concerning the existence of a very rich capillary bed lying beneath the so-called impermeable epithelium that lines the mammalian urinary bladder. Not only for a proposal that was primarily anatomic but, to add to the question, a proposal including an experiment that was very simple to be nice. Yet, I/we received a lot of money based on a recommend of full acceptance from the peer review! Once we were found out by the folks from upstairs at NIH, however, the word came down that in order to get even consideration for an extension, I would have to apply the new methods of "Molecular Biology" to our problem. Thus, I outfitted an appropriate lab, and began to educate myself in a methodology that was based on an instrument called a "PCR." In the process, I discovered the truth that the primers were the key to success, and no one would share her/his method with anyone else. Time and pure luck paid off to my best skill - that I have always called, "LIBRARY!" Once in a satisfactory place, and with new-found confidence in the primary generator I had found, I received a visit from a colleague who had been recommended to me for help with his primer problem. Always one to share my knowledge, I spent about 90 minutes rustling up a set of primers that were perfect for his target using the program "BLAST" from NIH. The outcome of my contribution was ultimately a surprise report of a new human gene called, "TERE1" then, and "UBIAD1", now. In context, I would have appeared last in my class - at any level - as one likely to do research at the University of Pennsylvania, and more recently that I, an anatomist/histologist, would EVER have found himself associated with a gene on the human genome. Thus, like the axiom -"you can'!
t win if
you fail to play" - 'if you are not given the opportunity to discover, there is little chance that you will discover - and even then, you likely must be a team player.'

Finally, there is little magic in science that doesn't rise from experience, but in life, the magic may be common, plentiful, and wonderful.

God bless, and Happy New Year - which ever you follow,

Fred Monson

Frederick C Monson, PhD
Center for Microanalysis and Imaging, Research and Training (CMIRT)
West Chester University of Pannsylvania
West Chester, PA, 1938
610-738-0437
fmonson-at-wcupa.edu
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